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Teaming up to interrogate genes about ADME
April 2011
BRANFORD, Conn.To help scientists capture known and unknown functional mutations associated with core sets of pharmacokinetic and pharmacology genes, Roche subsidiary 454 Life Sciences and Lexington, Mass.-based RainDance Technologies Inc. have signed a deal for developing and commercializing a targeted next- generation sequencing (NGS) solution for simple and cost-effective investigation of drug absorption, distribution, metabolism and excretion (ADME).
As part of the collaboration, RainDance and 454 Life Sciences plan to commercially launch a core ADME gene screening research panel that will be made available on both the 454 Life Sciences' GS Junior and GS FLX Systems. Financial terms of the deal were not disclosed.
"For the first time, researchers can look beyond the mutations found on current genotyping panels and discover novel chromosomal changes and rare mutations associated with drug metabolism and adverse drug events," said Roopom Banerjee, president and CEO of RainDance Technologies, in a news release about the deal. "Through our collaboration, Rain Dance and 454 Life Sciences are combining the power of our microdroplet technology with 454 Life Sciences' proven next-generation sequencing platforms to provide a robust and economical ADME sequencing solution."
Current ADME research products are primarily limited to genotyping common alleles, the companies note, and therefore lack the sensitivity, breadth of genomic coverage and ability to identify previously unknown variants that are important to drive scientific discovery and routine use.
RainDance and 454 Life Sciences maintain that their collaboration will enable researchers to interrogate a set of core pharmacokinetic and pharmacology genes, while at the same time to detect known and unknown functional mutations associated with drug metabolism and response.
"We started collaborating on a new ADME solution concept about a year ago," recalls James Brayer, RainDance's product manager for targeted sequencing. "The idea was born out of a mutual desire to bring the power of next-generation sequencing to ADME research. We have a number of mutual customers who are interested in an easy- to-use solution that targets all of the core ADME-related genes in a high-throughput fashion."
The impetus to deal with that concern wasn't simply a business opportunity for the two companies, but a push they felt from customers, adds Ulrich Schwoerer, 454 Life Sciences' head of global marketing, who tells ddn that "companies and research organizations involved in the discovery, development and manufacture of medicines are under increasing pressure to bring down costs and, more importantly, ensure the safety of products that are bound for, or already in, consumer markets."
In choosing 454 Life Sciences as a partner, RainDance was remaining true to its commitment to work with best-in-class partners, Brayer says, "to enable a number of new targeted sequencing applications that would benefit from the single-cell and single molecule resolution that is possible with our microdroplet-based platform. We also believe that our platform plays a central role in further advancing the routine use of next-generation sequencing in research areas that have been difficult to examine using previous technologies."
Looking at his own company's strategic goals, Schwoerer says that 454 Life Sciences' vision is to enable researchers, particularly in the area of medical research, to do their jobs far better and more efficiently.
"For the time being, targeted sequencing offers the most cost-effective and tractable approach to translate the use of genomic information into a medically relevant result," he says. "Targeted resequencing technologies such as RainDance's provide an opportunity to focus on the genomic regions with the highest likelihood of impacting research in a cost- and time-efficient manner."
The two men say their companies continue to evaluate other potential complementary applications and workflows, adding that they view this new ADME panel as a proof of principle illustrating the capability of coupling RainDance's targeting methods with 454 Sequencing Systems. RainDance's ability to custom-design PCR amplicon panels that fit into the 454 Sequencing workflow with minimal effort means that researchers can use this panel as a starting point, but also have the flexibility to target any region of the genome they are interested in, they say.
RainDance's proprietary primer design method reportedly enables highly accurate, consistent and reproducible next- generation sequencing in ADME research, which is required for large-scale drug metabolism studies. 454 Life Sciences' GS Junior and GS FLX Systems are said to provide high-quality, long-sequencing reads which enable higher levels of coverage, accuracy and quantitation with fewer dropouts, as well as detection of a wide range of genomic variations, including SNPs, insertions, deletions and multinucleotide polymorphisms.
"Our collaboration with RainDance will result in solutions that better equip scientists to leverage the GS FLX and GS Junior Systems to effectively predict and understand drug activity early in the drug development process," said Christopher McLeod, president and CEO of 454 Life Sciences, in a news release about the deal, adding that adverse drug events represent more than two million hospitalizations and approximately $1 billion in healthcare cost burden annually in the United States alone.
RainDance and Ambry Genetics ink ADME analysis deal
LEXINGTON, Mass.RainDance Technologies Inc. also recently announced that it will collaborate with Ambry Genetics on the development and commercialization of a comprehensive drug absorption, distribution, metabolism and excretion (ADME) genetic screening panel for use on next-generation sequencing (NGS) systems.
The announcement was made at the Society of Toxicology's 50th Annual Meeting in Washington, D.C. Financial terms of the collaboration were not disclosed.
The new ADME panel will provide scientists with the capacity to perform sequence analysis of entire coding regions associated with more than 220 key drug metabolism-linked genes, including transporters, receptors, regulators and all pharmacodynamic and pharmacogenetic genes, as well as all U.S. Federal Drug Administration (FDA)-identified pharmacogenomic biomarker genes. The panel, provided as part of Ambry's ADME sequencing service, will leverage RainDance's primer design methods and microdroplet-based RDT 1000 platform, which according to RainDance provides the highly accurate, consistent and reproducible results required in ADME research and for most FDA drug applications.
"Scientists interested in analyzing ADME-linked genes can now look far beyond what existing genotyping panels reveal and discover the novel chromosomal changes and rare variants associated with drug metabolism and molecular pharmacology," says Dr. Darren Link, RainDance's co-founder and vice president of research and development. "Through our collaboration with Ambry Genetics, we are providing a solution that puts the most advanced ADME research tools within reach of the many research organizations focused on reducing the significant costs associated with failed drug trials and avoidable hospitalizations."
Code: E041110
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Each week we run the box office numbers through our proprietary formula that tabulates receipts, the film's budget, the number of screens and critical response to see not just who made the most money, but who's leading the PopcornBiz Championship Series.
After 21 consecutive days as the #1 movie in America, "The Avengers" were finally knocked off their perch by Will Smith's "Men in Black 3." Before we hail "MIB3"'s triumph, let's take a minute to consider "The Avengers"'s run: in the course of racking up $523 million so far, "The Avengers" enjoyed the best 1st and 2nd weekend of all-time, and the film is poised to pass "The Dark Knight" ($533 million) for highest-grossing comic book adaptation and the #3 spot on the all-time list. It was one of the few films of recent vintage that completely lived up to its hype--both commercial and critical.
But there was no denying "MIB3" this weekend, which overcame an unfinished script, a long break in production, a lot of ill will, a 10-year layoff and a widely reviled second installment to rake in an impressive $70 million. Let's look at the top 10, via Box Office Mojo:
U.S. & World
2. The Avengers - $46,878,000
3. Battleship - $13,769,000
4. The Dictator - $11,755,000
5. Dark Shadows - $9,405,000
6. Chernobyl Diaries - $9,300,000
7. What to Expect When You're Expecting - $8,850,000
8. The Best Exotic Marigold Hotel - $8,225,000
9. The Hunger Games - $2,850,000
10. Think Like a Man - $1,800,000
As great as "MIB3" did, though, when you run the the box office numbers, budgets and critical responses through our proprietary formula, Smith and Co. finished a distant third, with a British indie romcom starring a bunch of 60-somethings reigning supreme in our Popcornbiz Championship Standings:
1. The Best Exotic Marigold Hotel - 1020
2. The Avengers - 654
4. Think Like a Man - 55
5. The Dictator - 48
6. The Hunger Games - 42
7. Chernobyl Diaries - 24
8. What to Expect When You're Expecting - 12
9. Dark Shadows - 11
10. Battleship - 7
"The Best Exotic Marigold Hotel," which stars Judi Dench, Tom Wilkinson and Bill Nighy as a group of pensioners who head off to India, took in $8.2 million this week, after expanding into another 879 theaters. People often talk about how grossly under-served blacks and Latino audience are at the movies--and they definitely are--but people over 60 are probably even further overlooked. This would go a long way toward explaining how it is that "Best Exotic" is doing so well. Just watch--you think we're coming out of a vampire glut? Beware the old people!
Next week the greybeards will be confronted with "Snow White and the Huntsman," starring Kristen Stewart, Charlize Theron and Chris Hemsworth.


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Microbe Magazine
Cover Image
Cover: Polarized light micrograph of crystals of the antibiotic streptomycin. Antibiotics can act as weapons or signals and mediate a broad range of interactions (see p. 282). (Image © David Parker/Science Source).
Microbe Magazine, Cover Image
Cover ImageVaccines are crucial for controlling the spread of influenza, but currently are only effective against a limited mix of strains. A universal vaccine remains elusive, but progress is being made (see p. 433). (Image © James King-Holmes/ Science Source.)
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What you need to know before you go
Flagstaff sits among the rugged, sun-baked landscapes of Northern Arizona, where majestic snowcapped mountains meet striking red deserts and rock formations. Unsurprisingly, it's a playground for outdoor recreation, from skiing the slopes of the Arizona Snowbowl, to hiking, biking, or venturing 90 minutes away to the iconic Grand Canyon.
Brimming with Route 66 nostalgia and Old West charm, Flagstaff itself provides a well-equipped basecamp during your adventures. Steeped in history, it's home to numerous drinking holes, locally run stores, and palate-pleasing restaurants. Close to town you'll find Bearizona – a natural safari park teeming with wolves and bears.
Flagstaff – nenechte si ujítCo se děje a co stojí za vidění
10 Best Things to do in Flagstaff
For those who love exploring the great outdoors, Flagstaff offers a wealth of things to see and do. This northern Arizona city of about 70,000 is the perfect base for discovering dormant volcanoes and ancient villages, hitting the ski slopes, going camping and hiking in the mountains, or gazing in wonder at the night sky at the same observatory where Pluto was first spotted.
Within the city, there's a surprisingly vibrant shopping and dining scene to enjoy in its historic downtown area, together with some excellent breweries serving up creative craft beers. Here's just a sampling of some of the best activities and sights to find in Flagstaff.
What are the best things to do in Flagstaff?Další informace
části města Flagstaff
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Další oblíbené destinace v oblasti United States of America
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Ruin Maniac
A Ruin Maniac is a Trainer Class that was introduced in Generation III. They appear as a stereotypical explorer, shown in Generation III as old archaeologists whose outfit resembles that of a Hiker's and shown in Generation IV as an old safari tourist and as a young archaeologist with a large green backpack.
Ruin Maniacs use a several types of Pokémon, including Ground, Rock, and Steel-type Pokémon. They can usually be found near caves and tunnels and rocky areas in-game.
Sprites[edit | edit source]
|RSE Battle Sprite
|FRLG Battle Sprite
|DPPt/HGSS Battle Sprite


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Electrical Help For Residential
Emergency Electrical Repairs
24/7 Support
Breaker Damage
When to Hire A Professional To Do Your Electrical Work
If it's broke, call someone to fix it. Sometimes we try to fix things ourselves but it doesn't turn out to well. Don't play when it comes to your electrical system. So, you have to know when it makes sense If you're a home buyer then you need to know how critical the inspection component is of your home. You can't skimp of quality when it comes to inspection of your electrical work.
Getting Home Improvements
If you 're planning a major overhaul of your home then listen up. This will save a ton of money and put ahead of the game when it comes to getting an overall view of your homes safety and electrical components.
How To DIY
You can't beat a seasoned veteran at spotting a problem. You may want to take a close look at work that was done in the home if it was done by a non-pros. To be worry from headaches simply get an electrical inspection and you should be good to go.
If Your House is a bit Aged
A couple of decades can do a lot to the most sturdy of homes. As much as we would like, nothing last forever. This is true for all types of houses that are built with old build models and wiring models. The best way to deal with this is getting a proper inspection.
If You didn't get a proper Inspection before buying you could begin for an expensive bill that could have been avoided If you already purchased the home and didn't go through the proper inspection you should call your local electrician immediately.
After Storm Damage
Believe it or not, a bad storm can cause some serious damage to your electrical system.
A lot of people don't think that this is possible but a bad enough storage could cause damages that you won't be able to see but they're definitely there
If you want to learn more about your electrical system then you need to call Lion Electricians.
If a component of your electrical system fails, you might start experiencing problems out of the blue. And if you keep experiencing the same problem over and over, even if it's something minor like light bulbs burning out too quickly, it could be a sign of a dangerous underlying issue. In either case, an electrical inspection is the proper way to find out what's going on.
When Your Homeowners Insurance Company Requires It
Depending on your insurance company and policy, you may need to submit to periodic inspections of your electrical system and other home systems. If this is the case, you will probably also be required to promptly fix any problems that are discovered or risk having your policy canceled.
Scheduling an electrical inspection shouldn't break the ban, but the repairs to the electrical system can protect your home from a fire or anything shorting. If any of these circumstances apply to your home, call your local Lion electricians today and schedule an inspection right away.
Lion Electricians has been the best choice that I've made when it comes to hiring a competent electrician that's fair and affordable.James P,
If you're looking for the best company to do your electrical work for commercial work then you've found your guys. Thanks Again!Harold G.


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Three independent bookstores are suing online bookseller behemoth Amazon and the publishers known as the Big Six, claiming that the group has created a monopoly in the sale of e-books.
Fiction Addiction, a store based in Greenville, S.C.; Posman Books, a New York City store which has three locations; and Book House of Stuyvesant Plaza of Albany, N.Y., filed the class action lawsuit.
The suit, which was filed in New York, takes aim at Amazon, Hachette, Penguin, Simon & Schuster, HarperCollins, Random House, and Macmillan.
The stores that filed the suit say that they represent "all independent brick-and-mortar bookstores who sell e-books."
The root of the complaint centers on digital rights management, which makes it difficult for a reader to switch an e-book from one e-reading device to another – for example, to move a book from a Kindle, the Amazon e-reader, to a Kobo, the one sold by indie bookstores.
The indie bookstores are saying that's hurting their business.
"We are seeking relief for independent brick-and-mortar bookstores so that they would be able to sell open-source and DRM-free books that could be used on the Kindle or other electronic ereaders," Alyson Decker of Blecher & Collins PC, who is serving as lead counsel for the bookstores, told the Huffington Post.
The suit claims that Amazon entered into confidential agreements with the six publishing houses. E-books sold by all six publishers come with the digital rights management lock that makes it difficult to move them to different devices.
"Currently, none of the Big Six have entered into any agreements with any independent brick-and-mortar bookstores or independent collectives to sell their e-books," the plaintiffs write in their suit. "Consequently, the vast majority of readers who wish to read an e-book published by the Big Six will purchase the e-book from Amazon."
In addition to damages, the three indie bookstores also want an injunction which would "prohibit ... Amazon and the Big Six from publishing and selling e-books with device and app specific DRMs and further require ... the Big Six to allow independent brick-and-mortar bookstores to directly sell open-source DRM e-books."
Simon & Schuster representative Adam Rothberg told The New York Times, "We believe the case is without merit or any basis in the law and intend to vigorously contest it. Furthermore, we believe the plaintiff retailers will be better served by working with us to grow their business rather than litigating."
As industry newsletter Shelf Awareness pointed out, the lawsuit is somewhat baffling because the six publishers require digital rights management on e-books no matter who's selling them – including indie bookstores selling e-books for the Kobo device. In addition, the bookstores state that only e-books sold to users by Amazon will work on Kindle devices, which is not the case – users can read e-books from other sellers on their Kindles. (Transferring books from a Kindle to another device is tricky, though the technically savvy may be working on a solution not sanctioned by the companies.)
Another confusing point, wrote Shelf Awareness, is that while the bookstores charge that "the vast majority" of readers purchase e-books from Amazon, they fail to point out that Kobo, the company that produces the device sold by indie bookstores, also sells e-books obtained from the Big Six publishers.
In addition, Cory Doctorow, a writer for the blog Boing Boing, points out that the bookstores incorrectly used the term "open source" to describe what they want, which is e-books that would work across e-readers. "Open source" is defined as a computer program whose original code is made available to users, allowing them to change the program.
"For some reason, they're using 'open source' as a synonym for 'standardized' or 'interoperable,'" Doctorow writes. "Which is to say, these booksellers don't really care if the books are DRM-free, they just want them locked up using a DRM that the booksellers can also use. There is no such thing as 'open source' DRM – in the sense of a DRM designed to run on platforms that can be freely modified by their users.... I wish they'd actually bothered to spend 15 minutes trying to understand how DRM works and what it is, and how open source works, and what it is, before they filed their lawsuit."
The suit from the three independent bookstores follows after the Department of Justice sued several major publishers, stating they had worked with Apple to make e-books more expensive. Hachette, Simon & Schuster, Penguin, Macmillan and HarperCollins all agreed to settle, leaving Apple as ithe only defendant in the suit. The trial is scheduled for June.


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Skip to Search
|Library Catalog | Databases | Ask a Librarian | Remote Logon | Search
|Library > Special & Area Studies Collections > General Manuscripts > Finding Aids
The Florida Accounting Archives were established in 1971 through gifts made and solicited by Dr. Williard E. Stone, Professor of Accounting, ca. 1960-1980, and Chairman of the Department of Accounting. A dedication program is included in the Elmer G. Beamer Papers.
The collection is composed of twelve manuscript groups relating to the history of accounting:
Group 1:
Klein, Hinds & Finke. Records of the Kreuger Match Case (International Match Corporation), 1924-1940 (bulk: 1932-1940). Papers regarding the bankruptcy of the International Match Corporation (IMCO), following the suicide of Ivar Kreuger in 1932. Accounting firm of New York. Part of Accounting Archives. 8 boxes. 3.5 ln.ft.
Group 2:
Johns, Ralph S. Collection of Accountant Certificates, 1876-1968 (bulk: 1930-1968). Part of Accounting Archives. 2 boxes. 0.75 ln.ft.
Group 3:
Chambers, Raymond J. Papers, 1963-1973. Professor of Accounting, University of Sidney. Bibliography of works, typescripts of accounting articles. 1 box. Part of Accounting Archives.
Group 4:
Paton, William Andrew. Papers, 1944-1972. Correspondence, manuscripts, research drafts, etc. Member of the Accounting Hall of Fame. Part of Accounting Archives. 2 boxes.
Group 5:
University of Florida Department of Accounting. Collection, 1963-1969. Selected departmental correspondence with prominent accountants. Williard E. Stone, chairman of the department, is the principal correspondent. Part of Accounting Archives. 1 box.
Group 6:
Beamer, Elmer G. Papers, 1949-1972 (bulk: 1962-1972). American Institute of Certified Public Accountants correspondence, notes, seminar reports; Committee on Continuing Education correspondence, memos, reports, speeches; American Accounting Association; Beamer speeches; Cleveland Chamber of Commerce Committee on Education. Part of Accounting Archives. 3 boxes. 1.3 feet.
Miscellaneous Accounting Papers, 1837-1971. Miscellaneous, small collections that form part of the Accounting Archives. O.V.F. Blythe. John Faire. Louis Goldberg. Maurice E. Peloubet. Procter and Gamble Company. George L. Wright. 2 boxes. 0.8 feet.
Group 8:
Birley and Company. Records, 1810-1877. Accounting Records of Charlton Mills, Birley & Co., and Charles MacIntosh & Co. Inventories, ledgers, and correspondence. Part of Accounting Archives. 2 boxes. 0.8 ln.ft.
Group 9:
Eaton, Marquis G. Papers, 1949-1957. Speeches, committee records, articles and correspondence covering his tenure as president of the Texas Society of Certified Public Accountants and the American Institute of Certified Public Accountants (AICPA). Part of Accounting Archives. 15 boxes, 6.25 ln.ft.
Group 10:
Foster, Louis O. Papers, 1929-1961. Professor of Accounting, Dartmouth University. Author of several accounting textbooks. Manuscripts of published and unpublished works, including his doctoral dissertation. Part of Accounting Collection. 4 boxes. 1.5 feet.
Group 11:
Grady, Paul. Papers, 1938-1967. Copies of published and unpublished papers by Paul Grady on accounting (originals deposited in the Hoover Institution, Stanford University). Part of Accounting Archives. 1 box. 0.4 ln.ft.
Group 12:
Jennings, Alvin R. Papers, 1948-1964. Manuscripts of papers delivered at accounting society meetings. Part of Accounting Archives. 2 boxes. 0.8 ln.ft.
For further information, please contact: Special Collections Access Services.


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03/19/2019, 5:30 PM to 6:30 PM
Help the big brother or big sister prepare for the changes ahead. Activities, discussions and practice are used in this session to teach children aged 3 and older about their new role as siblings. Your child will learn about such topics as:
- The emotions associated with becoming a big sister or brother
- Newborn appearance and behavior
- Special rules about new babies
He or she will also practice holding and diapering the baby. An age-appropriate tour of our facility is included so your child may become comfortable with the hospital and visiting routines. This class does not prepare children to participate in the birth.
To ensure that all of our families remain safe and healthy, class schedules may change during peak flu seasons.
October 06, 2020|6:00 - 7:30 PM
October 06, 2020|6:30 - 8:30 PM
October 07, 2020|2:00 - 5:00 PM
October 18, 2020|5:30 - 8:30 PM


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Every Saturday Morning @10:30 am | MJE West | Blue Room
A great space to meet YJPs, have a meaningful conversation, and enjoy some pastries! No need to sign up, just come on by. Led by Rabbi Joshua "Shuki" Klein and Ruthie Braffman.
(Services begin 9:30am, Sermon at 11am, kiddush at 11:45am)


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A Solution from Purgatory (for the Matrix from Hell)
This post attempts to recount some of my thought process which eventually (more than a year later) lead to the Matrix from Hell puzzle I posted about a few days ago. If you don't want to know my answer, don't read the rest of this post. My final solution is at the bottom.
You have been warned.
Below is a list of my general thought process and the main ideas/revelations/halucinations that I know helped me to my solution. An annotated matrix is at the bottom with some of the ideas highlighted in different colors.
- Should indices start at 0 or 1?
- Values along either axis count up by 1 in both directions when the other =0
- Values are duplicated over the f(x)=y axis –> Order of indices given to function is inmaterial since they can be swapped
[The RED 5 can be seen duplicated over the diagonal]
- Calculation of max/min index is O(1) so stop worry about it and "forget" the bottom half of the matrix (assume X>= Y)
- Values generally increase as they go right/down but not always
- f(x,x)=0, f(x,0)=x
- Values are symmetrical perpendicular to f(x)=y IFF the bounding square in question is a power of 2
- "Quad" patterns can be seen repeating for every power of 2. In each "Quad" the top left quadrant is the same as the bottom right and the top right is the same as the bottom left.
[Each "Quad" of the example matrix is outlined in BLUE with the quadrants in LIGHT BLUE. PINK shows the repetition]
- power of 2 repeating nature of the values leads to thinking of binary trees
- binary trees leads to thinking about a possible solution which could run in O(log2 n) time:
- In a nut shell, for a given (X,Y), algorithm would find smallest boudning power of 2 including X and would walk back "Quad"s by each successivly smaller power of 2 "Quad" until the last one is met. For each "Quad" determine which quadrant the value lives in and remap to root values.
- Debate if this would really work for large numbers and if it could be reduced to constant time function by math that I have forgotton from college/HS
- Dismiss b/c I can't remember a way and base idea is > O(1)
- Binary trace back algorithm idea makes me realize that it can be also looked at as the "binary" representation of a number (e.g. 01011011010) where each 1 represents a "Quad" where the value was in the upper right or bottom left and vica-verca.
[Following the matrix value at (5,6) can be seen ORANGE. The value 3 is in the bottom right of the 1st "Quad", then BL, & TR. {BR BL TR} => {101} => {3}]
- Remembered that f(x,y)=0 IFF x==y …. which means that difference between X and Y is important to value of f()
- Eureka!
- F(X, Y) = X xor Y
- solution typed above in white on white to protect the innocent. highlight to view.


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Spotlight spaghetti dinner March 29
SOLON– Mark your calendars for Thursday, March 29, for the annual Solon Spotlight spaghetti dinner. The event will be held in the high school commons immediately before the Solon Musical Showcase concert. Spaghetti, salad, bread, drink and dessert will be available for $8 for adults and $5 for children 10 and under. Serving times are 4:45 until 7 p.m. Diners will enjoy pre-concert entertainment by the high school jazz bands, jazz choirs and the middle school show choir. Starting at 7:30 p.m. there will be a progressive showcase of musical talent in the high school gymnasium from the fifth grade choirs and band continuing up to the high school choirs and band. All proceeds from the spaghetti dinner will benefit K-12 performing arts programs at Solon schools.


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Cory Gunz & Freeway On Da Spot Freestyle Pt. 1 [16 Min. Invasion Radio]
Freeway And Cory Gunz - ON DA SPOT PT1
Please click the "Report" button below if the video on this page is not working properly.
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Geriatric Care in Skokie, IL
As we age, it becomes more important than ever to schedule regular appointments with a health care professional. A geriatric care physician can help you maintain the quality of your lifestyle.
Your doctor can create a physical examination regimen and wellness plan to fit your individual needs. The following is a brief overview of some of the areas of elder care that exist.
Coping with a Loss of Memory and Cognitive Function
Over time, our brains cells and the links between them begin to degenerate. If this process occurs too quickly, it can lead to a condition known as dementia. Dementia is a broad topic that can describe a number of ailments such as a diminished ability to think, remember things, or make decisions. The most common cause of dementia is Alzheimer's disease .
Alzheimer's can begin as short-term memory loss, but can eventually lead to disorientation, trouble speaking, and an inability to concentrate. It may even bring about changes in your personality.
There are a number of tests your doctor may perform to help identify a change in the neural impulses within your brain. These can range from neuropsychological testing to imaging technologies such as an MRI or CT scan.
There is no cure for Alzheimer's or most other forms of dementia, but there are ways to slow down the degeneration of brain cells. Your doctor may advise you on a number of intellectually stimulating exercises that may include speech therapy programs and engaging in social interactions.
Eyesight and Hearing Loss
There are four main types of age-related vision impairments:
- Glaucoma: A peripheral loss of vision
- Macular degeneration: Center vision loss
- Diabetic retinopathy: Spotty vision
- Cataracts: Blurred vision
Any of these conditions can be a challenge to overcome, but with the help of a health care professional, none of them will keep you from doing the things you love. Your doctor may recommend assistive devices as well as changes that can be made within your home that will make it easier to perform everyday functions without the risk of falling or other injuries.
Nutrition and Exercise
As with any point in your life, proper nutrition and regular exercise are essential in your elder years. Diet plans include healthy eating suggestions and the correct balance of supplements to prevent conditions such as a loss of bone mass, hypertension, and constipation.
Fitness programs focus on building up your strength, endurance, and flexibility. In addition to building up your muscles and tissues, the right exercise routine can also improve a variety of conditions. Some of them include:
- Improving your immune system
- Lowering your risk for heart disease and high blood pressure
- Preventing the loss of bone mass
- Encouraging digestive health
- Lowering the risk of dementia
Rita N. Oganwu MD, SC
Address20303 S Crawford Ave
Ste 110
Olympia Fields, IL 60461
(708) 435-2381
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The search found 310 results in 0.01 seconds.
Rights and rhetoric clash in abortion politics, with Susan Markens, Katrina Kimport, Drew Halfmann, Kimala Price, and Deana A. Rohlinger.
LGBT educators struggle to balance professionalism and pride in the classroom, splittling, knitting, or quittting, in the words of the authors.
When a devastating earthquake and tsunami hit japan in 2011, the effects were felt by over a million expatriates worldwide.
All sociologists recognize that social constraints affect individuals' outcomes. These effects are sometimes relatively direct. Other times constraints affect outcomes indirectly, first influencing individuals' personal characteristics, which then affect their outcomes. In the latter case, the social becomes personal, and personal characteristics that are carried across situations (e.g., skills, habits, identities, worldviews, preferences, or values) affect individuals' outcomes. I argue here for the importance of both direct and indirect effects of constraints on outcomes.
This article investigates whether men's sexual behavior is influenced by the sex of their firstborn children and, if so, at what stage of firstborns' development this occurs. Using standardized data from 37 Demographic and Health Surveys (N = 61,801), I compare the sexual activities, sexually transmitted infection symptoms, and sexual ideologies of fathers with firstborn sons and fathers with firstborn daughters. I also explore whether fathers' attitudes mediate the effects of firstborn sex.
We examined differences between sexual minority women's (SMW's) sexual identity and sexual behavior or sexual attraction as potential contributors to hazardous drinking across a 10-year period. Data are from a longitudinal study examining drinking and drinking-related problems in a diverse, community-based sample of self-identified SMW (Wave 1: n = 447; Wave 2: n = 384; Wave 3: n = 354). Longitudinal cross-lagged models showed that SMW who report higher levels of identity-behavior or identity-attraction discordance may be at greater risk of concurrent and subsequent hazardous drinking.
Existing research emphasizes the importance of group identification and perceived similarity in the development of group consciousness. Intersectionality suggests that for many women, a political consciousness of gender may also stem from experiences with race, ethnicity, and sexuality and may be interconnected with a consciousness of other forms of inequality. This study analyzes data from a recent national survey to investigate how race, ethnicity, and sexuality intersect with women's gendered political consciousness.
Although research has established a very strong relationship between the presence of a psychiatric disorder and victimization in prisons, some gaps remain in our understanding. This study considers the importance of gender differences in this relationship. Estimates based on the 2004 Survey of Inmates in State and Federal Correctional Facilities show that psychiatric disorders have a stronger relationship with victimization among male inmates than among female inmates. Yet the size of the gender difference varies greatly depending on the specific disorder.
Cancer is a life-changing condition for many American seniors, and a growing body of literature is assessing the mental health implications of living with the disease. This article builds from the well-known buffering hypothesis with insights from recent cancer research to investigate whether social networks moderate the association between cancer and mental health for older men and women.
This study explores gender, activity, and well-being among people with mental illness attending day centers in Sweden. Based on survey data of 215 attendees, this study applies the concepts of doing gender and regulatory regimes to analyze the relationship between being involved in gendered activities and well-being. The results show that while both male and female participants are involved in gender-neutral activities, men are less likely to engage in women-dominated (WD) activities while women are more prone to engage in men-dominated (MD) activities.


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Just why Jessops failed has been put down to a number of factors.
This article repeats itself but says it was the suppliers tightening credit terms.
This article says it was the poor knowledge and level of service in the stores. It is anecdotal about experiences in a London shop but in comments, some elsewhere in the UK backed that up and some disagreed, particularly Jessops employees.
Shopping has changed to more online after Amazon, the recession has got people jittery, e.g. suppliers and there are a hell of a lot of poor employees. But there are some very good ones.
Some blame the High Street in general. One commenter wrote:
At the end of the day, they grew too big to cope with actually training their teams properly – on products that no longer have great profit margins to cover the huge expense of those teams and premises.
He asked why they overcharged on memory cards.
H/T haiku


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Smash may have gotten axed by NBC last May, but the show's music lives on.
Hit List, one of two fictional in-the-works musicals from the drama series, is set to make its real-life stage debut this December, The New York Times reports.
TV musicals: The highs and lows
The cast will feature former Smash stars Jeremy Jordan (Jimmy), Andy Mientus (Kyle) and Krysta Rodriguez (Ana). No word yet if the actress behind Hit List's leading lady, Katharine McPhee, will also participate. However, there will also be "special appearances by several of Hit List's composers and additional performers to be announced at a later date," according to the newspaper.
Broadway lounge 54 Below will showcase Hit List for one night only on Dec. 9. The musical, which was only seen in snippets in various episodes, is described as "a modern fable about identity and the price of fame" that "tells the tragic love story of a wannabe pop singer who transforms herself to become a star and the hidden songwriter she uses to get there — but at what cost?"
Fall TV popularity contest 2013: Which new shows won you over?
In addition to the songs made famous on Smash, this special performance will also include additional tunes that were intended for Hit List, but never made it to air.
Hit List was introduced in the second season of the show as an edgier, Rent-like alternative to Bombshell, Smash's original fictional musical about the life and loves of Marilyn Monroe.


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Mango Indian Cuisine
2920-2922 Merrick Road, Bellmore
(516) 809-9927
While Mango Indian Cuisine only opened several weeks ago, management behind it is quite well seasoned. Sewa Singh, who also owns House of India in Huntington Village, boasts 20 years in the restaurant industry. Joining him at Mango are his son, Virpartap Singh, and his daughter, Simran Kaur. Still a work in progress — as they await table candles and embellishments for the walls — the intimate space is sleek yet cozy, offering rave-worthy Northern Indian food and top-notch service.
Their expansive menu had options for everyone, from meat aficionados and seafood lovers to vegetarians. Lunch Special — soup, Nan, chutney, rice and a main dish — is $11. From the dinner menu, soups, chutneys, salads, breads, and hot appetizers range from $3 to 13. Entrees (chicken, lamb, seafood, vegetable, Tandoori, and Biryani rice specialties), are $15 to $27. Desserts are $6 and $7. Children can be accommodated.
How mild or spicy your food will be is your choice ... just ask. If there is something you'd like that's not on the menu, if they have the ingredients, they are happy to prepare it. Management is friendly and very accommodating. Portions are quite generous.
We started with Mango Lassi, a refreshing, creamy yogurt beverage. Mango Special Variety Tray wowed us with a sampling of hot appetizers — crisp vegetable and meat Samosa, Bhujia (vegetable fritters), potato, cheese and chicken Pakora (batter fried), and a basket of Papadam (spicy Indian crackers) with three chutneys on the side.
Entrees include Basmati rice. Chicken Tikka Masala, boneless white meat, arrived in delicious sauce made with cream, tomato, green pepper, onion and spices. Lamb Mango, so tender, no knife needed, was dressed in a sweet brown mango/saffron sauce. Saag Paneer, a hearty blend of spinach, homemade cheese and curry, was amazing on nan bread or mixed with rice. Garlic Nan, one of 15 freshly baked breads, was served straight from the oven.
All Tandoori items (chicken, fish, lamb, cheese) are cooked on skewers in a charcoal clay oven, served with rice and curry sauce on the side. Mixed Tandoori platter offered a variety — several types of chicken, lamb Boti, and Seikh kebab — minced lamb blended with Indian spices.
Desserts are all made on premises. We shared and enjoyed Rasmalai, a traditional favorite – soft, sweet Indian cheese in cream sauce, served chilled. Gulab Jamun, Kulfi, and homemade ice cream are also on the menu.
Hours are 11:30 a.m. to 10 p.m. Tuesday through Thursday. 11:30 a.m. to 10:30 p.m. Friday and Saturday, noon to 10 p.m. on Sunday. They are closed on Monday. Reservations are suggested. Delivery, catering and take-out are available.
• Mango Lassi
• Garlic Nan
• Mango Special Variety Tray (assorted appetizers)
• Chicken Tikka Masala
• Lamb Mango
• Mixed Tandoori Platter
• Saag Paneer
• Rasmalai


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Bed and Breakfast Edeforsvägen 40, 96024 Harads Norrbotten Sweden
All properties below being sold as one lot.
Large Fully Furnished Family House:- Edeforsvägen 40, 96024 Harads.
The Main Business - Brooke's B&B:- Utsiktsvägen 2, 96024 Harads.
Fully Furnished with up to 25 Beds, has been contracted to Migration for over 5 years.
The Garage:- Edeforsvägen 26, 96024 Harads.
2nd business opportunity (Car Wash / Car Service Repair)
The Old House (Repair Project):- Edeforsvägen 24, 96024 Harads.
Used to be the old Bakery, Good Solid building on a large plot of land.
(makes up the Garage Yard).
The House:- Edeforsvägen 40, 96024 Harads.
Ground Floor
Entrance hall with stairs to both 1st floor and basement. Large living room (split into 2 areas,
TV viewing area and Big Screen Cinema area), Large fully fitted kitchen with plenty storage,
(cooker, dish washer, Fridge Freezer etc), Access to rear porch and garden, Large dining room,
Bar area with its own WC, Good size office, Full bathroom with shower over bath.
Upstairs (1st Floor)
Large kitchen area (not used but makes lots of storage and a good dressing area). Master
Bedroom with access to balcony. 2nd Double Bedroom is used as part of the master bedroom
but can be split with the use of the large double sliding doors (could be a great nursery).
3rd Double Bedroom at the rear also has access to the balcony. 4th Double Bedroom at the front.
Full bathroom with shower over bath.
Downstairs - Basement. (Full Height)
Large Food Pantry with chest freezer & upright freezer. Hobby Room, Large Storage Room,
Games Room / Entertainment Room (wii + ps), Gym, Sauna with Shower Area, Wash Room /
Laundry with 2 Washing Machines & Dryer & Clothes Line.
The Rear Garden is totally enclosed and there is outside lighting all around the house, garden & garage. Mature trees, Decked area.
Double Garage, with storage room or workshop area at the rear, fully heated with ample electric sockets, ample space in main area of garage for 2 large cars & more.
Driveway Parking, for up to 7 cars easily, then space for up to 3 cars in drive next to house with car port.
Driveway Gate into rear garden to allow access if required.
The house comes fully furnished and ready to move straight into or to rent out. All beds, bedroom furniture, sofa's, units, tables, chairs, lamps, light fixtures, curtains etc will be left.
Brooke's HB Bed & Breakfast:- Utsiktsvägen 2, 96024 Harads.
Good sized fitted kitchen with 3 cookers/ovens, full size canopy extractor fan, large pantry ample fridge & freezer space.
T.V. Room with comfortable seating for about 10 people with 42" Flat Screen, DVD Player etc.
There is a Wash Room/Laundry with Washing Room & Dryer, A Small Office, Plenty Storage, a Recycling Room for Garbage.
Accommodation for up to 25 guests:-
Room 1 - Fully Furnished for 4 Guests
Room 2 - Fully Furnished for 2 Guests
Room 3 - Fully Furnished for 2 Guests
Room 4 - Fully Furnished for 2 Guests
Room 5 - Fully Furnished for 4 Guests
Room 6 - Fully Furnished for 4 Guests
Room 7 - Fully Furnished for 2 Guests
Room 8 - Fully Furnished for 5 Guests
There are 3 toilets with showers and 2 more toilets (1 of which is disabled). There is a large air
conditioning & purification system installed, lots of inside & outside storage, 2 clothes drying
areas, outdoor seating area, fire pit, parking for several cars, including 6 spaces with electric
hook up for winter, enclosed garden. There is an old (2000) 7.5 Ton Box Truck with Tail-lift at
the rear which gives lots of storage for extra beds, mattresses, Sofas etc (Dry & Secure Storage)
The Business (Brooke's) will also come with the contact telephone numbers which is advertised
in the 118 directory & known locally. The building has Wi-Fi Internet, Website & Facebook Page.
All the business accounts are done by Ekonomihuset in Boden.
Business Bank Account with Swedbank in Boden.
Business Registration Number.
Everything goes with the sale.
Brooke's also has 2 other properties which are to be sold as part of the business.
Garage @ Edeforsvägen 26
House (Old Bakery) @ Edeforsvägen 24
The Garage:- Edeforsvägen 26, 96024 Harads.
Large Garage with 2 Large Doors, it used to be the old Shell Petrol Station.
It has a maintenance pit for working under cars and there is water & electric.
There is easily room for 3 cars inside with workshop area.
The other part of the garage is a shop area, but the roof needs repaired at this side.
(Would make a great shop of office).
As a 2nd business the garage could easily be made into a car wash (nothing in the area, nearest is in Boden 46km away) or a small service garage or would suit a business with lots of equipment and storage.
There is a large forecourt at the front and to the side.
It is prime location in the centre of the village.
House (Old Bakey):- Edeforsvägen 24, 96024 Harads.
A good solid old building, in need of repair. The main structure is a solid timber construction and has a good weather proof roof. It is located on the next plot of land to the garage, it has a large plot which makes up the storage and large parking area for the garage.
The building could easily be repaired into a lovely little home (split the land with the garage). It could be made into a little shop or café with accommodation upstairs, again it has lots of parking. The bus stops directly outside this building so again location is great. Or just sell the building for timber & materials and keep the land for the garage.
Harads is a small village in the north of Sweden. It has a School & Kindergarten, Library, Swimming Pool & Gym, Petrol Station, Fire Station, Health Centre, Pharmacy, small Supermarket, Hardware shop, Electrical shop, Churches and more recently The TreeHotel opened here too. It is just 46km from Boden and 75km from Luleå and just outside Luleå you can find the Airport.
|City / Town:
|Edeforsvägen 40, 96024 Harads


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Final Fantasy XIII – Gil Farming Guide
PlayStation 3
______ _ _ ______ _ | ____(_) | | | ____| | | | |__ _ _ __ __ _| | | |__ __ _ _ __ | |_ __ _ ___ _ _ | __| | | '_ \ / _` | | | __/ _` | '_ \| __/ _` / __| | | | | | | | | | | (_| | | | | | (_| | | | | || (_| \__ \ |_| | |_| |_|_| |_|\__,_|_| |_| \__,_|_| |_|\__\__,_|___/\__, | __/ | |___/ __ _______ _____ _____ \ \./ /_ _|_ _|_ _| \ / | | | | | | > < | | | | | | / . \ _| |_ _| |_ _| |_ /_/ \_\_____|_____|_____| Final Fantasy XIII Gil Farming Guide By: Jesta & marter E-Mail: email@example.com -------------------------------------------------------------------------- Table of Contents -------------------------------------------------------------------------- 1. Introduction (ITR) 2. Chapter 9 Farming **Coming Soon** (CP9) 3. Chocobo Farming (CBF) 4. Mission 24 (M24) 5. Chapter 13 Ci'eth (C13) 6. Chapter 13 Ci'eth (Post Game) (CV2) 7. Adamtoise/Adamantortiose Farming (AF1) 7.01 Adamantoise Summon and Death (ASD) 7.02 Adamantoise/Adamantortoise No summon, Death (NSD) 7.03 Adamantoise Summon, Fang leader (AFL) 7.04 Adamantortiose Summon, Sahz leader (ASL) 7.05 Adamantortiose No Summon, Straight kill (AAA) 8. Frequently Asked Questions (FAQ) 8.01 Farming CP before Gil? (CBG) 8.02 Getting Catalogs? (GCT) 8.03 Stacking Catalogs? (SCT) 8.04 New Farming Methods? (NFL) 8.05 Farming TP? (FTP) 8.06 Stagger Gauge Resetting? (SGR) 8.07 Chapter 13 Farming? (13F) 9. Credits/Copyright Information (CC1) 10. Version History (VSN) -------------------------------------------------------------------------- 1. Introduction (ITR) -------------------------------------------------------------------------- The purpose of this guide is to detail the most popular Gil farming spots of the game. There is no real best spot, although certain spots are better then others depending on your current overall party strength. Mission 24 starts off the best. After that, Chapter 13 becomes the best, and finally when you are close to maxed out the Adamantoise in Eden becomes the best. I will try to provide raw numbers of each spot so that you can make your own choice as to which spot you want to try out. There is also an FAQ at the end, that will hopefully answer any and all questions that you may have about the guide, or about things related to Gil farming such as Catalogs. The Video's: I started a second play through so that I could capture videos of me doing the various farms at the lowest level possible. The video links are intended to help the people that need help, Not to show off my leet skills. I have a maxed out save file that I could make some clips from showing the fastest possible kills. But I feel that if you are that far into the game, you do not need my help. -------------------------------------------------------------------------- 2. Chapter 9 Farming **Coming Soon** (CP9) -------------------------------------------------------------------------- -------------------------------------------------------------------------- 3. Chocobo Farming (CBF) -------------------------------------------------------------------------- Method: Chocobo Digging Video Link: http://www.youtube.com/watch?v=-e5ya5MwtuY Image Link: http://img709.imageshack.us/img709/79/chocobogil.png Roles: None Items Required: None Optional Items: None Recommended Stats: None Approximate Gil per Hour: 467,100 Gil Description: This method requires no fighting, it only requires that you complete Mission 14 to unlock the Chocobo's. I will not going into details about the strategy, because the image says it all. I can explain how I came to the conclusion you can make 460,000 gil per hour here. A average run takes 4-5 minutes, in a run you get 3-4 items. The items have the following drop rates, 12.84% - Millerite - 1000gil 12.84% - Rhodochrosite - 2000gil 12.65% - Cobaltite - 3000gil 10.19% - Chocobo Plume - 20gil 9.94% - Chocobo Tail Feather - 50gil 9.52% - Gold Dust - 15,000gil 8.04% - Dawnlight Dew - 500gil 7.55% - Dusklight Dew - 850gil 4.75% - Gold Nugget - 60,000gil 5.00% - Moogle Puppet - 18,000gil 6.32% - Plush Chocobo - 35,000gil If you do the math that gives you 8,658 Gil per dig. With an average of 54 digs per hour. The Pros: No battling required. The Cons: Less Gil than some other methods. Gil is not guarenteed to reach optimal levels. -------------------------------------------------------------------------- 4. Mission 24 (CP9) -------------------------------------------------------------------------- Method: Repeatable mission Video Link: http://www.youtube.com/watch?v=T6LmvLOpQ38 Roles: Com-Rav-Rav/Com-Com-Rav/Com-Rav-Med/Com-Com-Com Items Required: None Optional Items: None Recommended Stats: None Approximate Gil per Hour: 540,000 at 10 Second Battle Times 360,000 at 30 Second battle Times 240,000 at 1 Minute battle Times (This includes the 30 seconds its takes to restart the mission) Description: This is the earliest available method of Gil farming worth covering. It is a very solid method even post game. You have to complete this mission to get past the Taejin's Tower arc of the story, so you know you are strong enough to do it. After you complete the Tower arc, take the elevator down to the next area, then turn around and go back. Take the elevator to the 4th floor and then take the sub elevator to the 5th tier. Here you will see a statue, he gives you this mission. The target spawns in this very room so there is no travel time. Kill it, and receive a reward that sells for 6,000 Gil. Rinse and repeat. *If you are beyond this point, Take the Ce'ith teleportal to the tower, ride the elevator to the 4th tier. Then take the Sub elevator to the 5th tier.* The Pros: Very easy, anyone in chapter 11 can do this. Good Gil gain, very steady with guaranteed returns. The Cons: With about a 500,000 max an hour return, Other methods will net you more Gil -------------------------------------------------------------------------- 5. Chapter 13 Ci'eth (C13) -------------------------------------------------------------------------- Method: Straight Farming Video Link: http://www.youtube.com/watch?v=zuCjxj3aNp4 With out commentary - http://www.youtube.com/watch?v=d-zIUo194cE Roles: Sab-Sab-Rav/Com-Sab-Rav/Com-Rav-Rav Items Required: None Optional Items: Having Collectors Catalog is recommended Recommended Stats: None Approximate Gil per Hour: 1,263,000 at 30 Second Battle Times 842,000 at 1 Minute Battle Times 552,500 at 2 Minute Battle Times (This includes the 30 seconds its takes to reset the spawn.) Description: This is the best method for raw Gil per hour. But after you beat the game the Gil per hour will take a massive hit. The reasoning will be explained later on however. Just before the portals at the start of Chapter 13, there is a save point with a pack of 3 Sacrifice Ci'eth, and a pack of 4. If you move off screen they both respawn. They are worth about 20k Gil each pack and take less than 2min to kill. They should eventually take 30 Seconds once you are geared up. You can also get preemptive attacks on them every time after a little practice if you are not geared/leveled. You start off with a Sab-Sab-Rav and just debuff them all. Once they are debuffed, switch to a Com-Rav-Rav and AoE all of them with Ruinga if you are leveled up, and your weapons are good. Then you can go Com-Sab-Rav and just start casting Ruinga at the very start with your Com. Switch to Com-Rav-Rav when they are all debuffed. There is no way to really tell when they are, you will just have to guess. What makes this so nice is that they are very susceptible to debuffs, and after they are pained and fogged they just wander around not attacking you. They are normally very hard to kill because of their massive HP and resistances to everything. But the debuffs make them lose their resistances, in addition to not attacking. When you first start here if you are weaker it might be a little tough, you might get a game over here and there if you get unlucky with the debuffs. Stick with it, after you get some levels and items it becomes VERY easy and has the best Gil gain in the game. Having trouble getting them to respawn?: The paths that go off to the sides are the ones you need to take to get them to respawn, you cannot use the main path to the portals or back towards Eden. This is why the farm take a massive hit after you beat the game. These two paths to the sides will disappear preventing you from respawning the packs properly and you have to save/reload. You can actually see the red dots reappear as you runaway from them, so you know exactly when they respawn. Having trouble getting the preemptive strike?: The pack of 3 it is possible to get the strike on the solo one right by the path and with some practice this is the best place to do it because it allows you to more respawn them more efficiently. However, if you are having trouble there, you can go around to the other side (near the path to the portals), and approach the two at the top. They will both turn their backs at the same time and it is very easy to preemptive them from here. The pack of 4 is easiest to get from the side path. One of them will wander very far out alone, wait for him to turn back to the group and hit him then. The math Drop (per Sacrifice): Perfume x1 or Scarletite x1 Price: 12500 for Perfume, 7000 for Scarletite Drop Rate: 28.1% Perfume (0.375 x 0.75), 25% Scarletite with Collector and no Connoisseur, assuming 5-star battle rating -------------------------------------------------------------------------- 6. Chapter 13 Ci'eth (Post game) (CV2) -------------------------------------------------------------------------- Method: Straight Farming Video Link: http://www.youtube.com/watch?v=0wr38hLD-_c Roles: Sab-Sab-Rav/Com-Sab-Rav/Com-Rav-Rav Items Required: None Optional Items: Having Collector's Catalog is recommended Recommended Stats: None Approximate Gil per Hour: 398,775 at 4:45 Minute Cycle Times (A cycle time includes the it takes to save/reload or warp in and out.) Description: This is the same monster as above at a much slower pace. After you beat the game you can no longer respawn the pack of 4 in the Cradle. The next best place for them is the long hallway just as you leave Cradle back into Eden hall. Here there are 6 Sacrifices that you can kill, then either teleport to Cradle and return, or save/reload to get them to respawn. The strategy is the same as above, except I would start as Com-Sab-Rav here, because the pulls are smaller. You can also just stay in cradle and save/reload the pack of 4 and 3. Which is just as good, but the time to save and reload is going to double or even triple your cycle times. So there you will only net about 400-500k gil per hour, at which point the Adamantoise is a much better target. The Pros: Easy The Cons: Less Gil than other methods -------------------------------------------------------------------------- 7. Adamtoise/Adamantortiose Farming (AF1) -------------------------------------------------------------------------- Next up is the most popular method of farming; Adamantoise/Adamantortoise killing. There are several ways to do this, So I will cover them all for completeness. Keep in mind the Gil per hour for these is more dependent on luck. Since the drop rate is much lower. I assume average kill times, time to reload the game, and the drop rate to find the Gil per hour for these. -------------------------------------------------------------------------- 7.01 Adamantoise Summon and Death (ASD) -------------------------------------------------------------------------- Method: Summon/Death Video Link: http://www.youtube.com/watch?v=Oo0RSSE0c44 Roles:Sab-Sab-Syn Items Required:None Optional Items: Haste boots, Level 2 Belladonna Wand, Energy sash Champions Bangle Recommended Stats: None Approximate Gil per Hour: 200,000-400,000 (Depending on luck) Description: This method is the first available to you, You only NEED the ability "Death" on Vanille. Any place with a Adamantoise near a save point is a good spot for this farm. The best places are the center of Pulse or Eden. This method is also the easiest, but most tedious of all the Gil farming methods. When the fight starts immediately Summon controlling Vanille. This will lower the beast to the ground destroying his legs and preventing him from attacking you. Make sure not to cancel your summon, let it attack along side you. Never activate the Gestalt mode. Start by adding Deshell and Deprotect to him, increasing the odds of your death. Then start spamming Death on him. Eventually your summon will leave, and your party will return. Hopefully the other Sab will get another Debuff or two up for you. Continue to spam Death and pray.If you are lucky enough it will work and he will die. I've found you can get 12 Deaths off with this method. Given the 5% chance of it working, + the bonus from the 2 Debuffs and the extra Sab in the party. This give you a 8% chance of the death working. meaning 1 in 12.5 should work. You might think this means you have around a 90% chance of succeeding but that's not quite right. I've found about 60% of them time you will get a kill, and the other 40% you will fail. If you fail, just retry. If you succeed then kill things to get your TP back. Save, reload and try again. The Pros: This is easy and can be done early. Once you get a 3rd Sab role, it becomes more efficient. The Cons: Very luck based, you can have a good streak or a bad one quite often. Farming the needed TP after battle is also annoying. Tips: 1) Go to settings and set the cursor to start on "Ability" at the start of battle. 2) If you manage to cast Haste, you can use Death 15 times before attacked. -------------------------------------------------------------------------- 7.02 Adamantoise/Adamantortoise No summon, Death (NSD) -------------------------------------------------------------------------- Method: Death Video Link: http://www.youtube.com/watch?v=HcWEU8XhQKs Roles: Sab-Med-Syn/Sab-Med-Med/Sab-Sab-Med(Optional) Recommended Items: Gaian ring's for each character. Optional Items: Haste boots, Level 2 Belladonna Wand Recommended Stats: More than 8000 HP Approximate Gil per Hour: 200,000-400,000 depending on luck. Description: The Adamantortoise is nearly the same as the Adamantoise, only he does less damage and has a smaller stagger guage. In this case the Adamantortoise is the better choice of the two to fight. This method is very similar to the Summon/Death method only this time you will try to stay alive while being attacked, instead of summoning in order to take out the legs. This requires a much higher level party, and some items to minimize damage. The Gaian ring's offer some very good damage reduction when starred. They are a must have. You start the fight the same as before, putting a few debuffs on him. Since you don't have as strict of a time limit here you can add more debuffs if you deem it worth while. Hope that your Syn will buff you with protect and shell. Once they do, switch to your Sab/Med/Med and heal up. Once you are healed enough, switch to a Sab/Sab/Med if possible, depending on your Crystogem levels. Spam Death and hope for it to work. An easy way to tell if you are strong enough to eliminate the summon is to simply try it. Either you will get owned really quickly by stomps, or you wont. The Pros: No more TP farming improves your cycle times greatly, allowing for more kills per hour. This method is also much less tedious than the Summon method. Having no time limit will guarantee much fewer failures. The Cons: Requires more items to prepare, and a much stronger party to handle it. -------------------------------------------------------------------------- 7.03 Adamantoise Summon, Fang leader (AFL) -------------------------------------------------------------------------- Method: Summon/Fang Video Link: http://www.youtube.com/watch?v=MGFJCIObXEo With out commentary - http://www.youtube.com/watch?v=bF1cvdrRVes Roles: Sab-Com-Com/Com-Rav-Rav/Com-Com-Com Items Required: None Optional Items: Haste/Brave/Faith Shrouds, Energy sash, Champions Bangle Recommended Stats: 1400 STR on each Com with Shrouds. Without Shrouds, 2000 will be needed. Approximate Gil per Hour: 334,000 at 8 Minute Cycle Times (420,000 if no shroud is used) (This includes the time needed to TP Farm) Description: For this method, the stronger Adamantoise is actually easier at first. Then the Adamantortoise becomes easier once your STR gets higher. Test them both out from time to time to determine for yourself which is better at your current STR. The reason behind this is the tortoise will stagger too early with your Summon still out, meaning you wont kill it in one stagger. Sometimes you can quickly restagger him if you have the ability to have a 3xRav Paradigm, but it is not worth the risk. As with the first summon method you summon your Eidolin right away. If you decided against using a Shroud, you can have Sahz start in Syn and try to get a few hastes prior to the second stomp. Then you can summon right before the second stomp. With your summon out, you should be in control of Fang as a Sab. Just let her Auto Debuff him until she has Deshell Deprotect and Imperil on him. You can do this manually to slightly speed this process up, remembering to cancel the remaining orders once the spell lands to save time by pressing B. After he is fully debuffed switch to your Com-Com-Com roles and Start attacking along side your Summon. As before, do not activate Gestalt mode. When his timer runs out he will fully heal your party and fly away. As he leaves, the Adamantoise will be about 3% from stagger. Use your Com-Rav-Rav Paradigm to stagger him. Swap to Com-Com-Com and attack for 2 rounds in this paradigm. Switch back to Com-Rav-Rav and attack once more before going back into Com-Com-Com. Every time you change roles your ATB is filled to full if at least 2 rounds have passed in your previous Paradigm. You can also set up a second Com-Com-Com and switch between the two Com Paradigms every two rounds to maximize damage. If he stands back up, you will fail. The Pros: This method is a guaranteed kill every time, so no more failures! The Cons - When using a Shroud its very counter productive to making Gil when you have to spend 12.5k just to try and get an Ingot. You will have to farm TP after each kill. -------------------------------------------------------------------------- 7.04 Adamantortiose Summon, Sahz leader (ASL) -------------------------------------------------------------------------- Method: Summon/Sahz Video Link: http://www.youtube.com/watch?v=FDmRe9uIur4 Roles: Syn-Com-Com/Syn-Rav-Sab/Rav-Rav-(Rav\Com)/Com-Com-Com Required Items: None Optional Items: None Recommended Stats: 900 STR on Sahz, 1400 on your other characters Approximate Gil per hour: 420,000 at 8 Minute Cycle Times (This includes the time needed to TP Farm) Description: Start of with a Summon once again. You can let him use a stomp before the summon if you can live through it, allowing Sahz some additional time to buff. Once your summon is out manually buff yourself, followed by a swap to a Com. Start Blitzing from there. Once your summon flies away, you will want to be in a Syn-Rav-Sab. Manually buff your team once again, (to avoid non offensive buffs). Haste/Brave are the best choices to use while buffing. Faith may be a good choice on Sahz as well. After you are buffed and he is debuffed, switch to your Rav-Rav-Rav and get him into stagger. Once he is staggered, start a Cold Blood and immediately switch to Com- Com-Com. This will give you the chain bonus of the Cold Blood and the damage bonus of a Com. As above you want to switch Paradigms every other round to maximize damage. Switch between Com-Com-Com and either Com-Com- Com or Rav-Com-Com for another cold blood if the stagger isn't high enough. Again, if he stands back up, you lose. The Pros: You can do this with out a Shroud sooner then the above method because of Sahz's Syn role. The Cons: You have to farm TP after each kill. -------------------------------------------------------------------------- 7.05 Adamantortiose No Summon, Straight kill (AAA) -------------------------------------------------------------------------- Method: Straight Kill Video Link: http://www.youtube.com/watch?v=Y-Z536tEosU Roles: Sab-Rav-Syn/Rav-Rav-Rav/Com-Rav-Med/Com-Com-Rav/Sent-Med-Med Required Items: Gaian ring's for each character. Optional Items: None Recommended Stats: Maxed in all 3 of your primary roles. Fang with at least one point in Rav. Approximate Gil per hour: 1,125,000 at 1.5 Minute Battle Times 750,000 at 3 Minute Battle Times 535,000 at 5 Minute Battle Times 344,000 at 8 Minute Battle Times (This includes 1.5 Minutes to Save/Reload + Start Battle) Description: To start, you will need to buff and debuff him. There are two strategies you can use in this battle, and they depend on which leader you select. Follow the appropriate strategy from here on out. Light: Light will take longer to kill the legs, but is able to stack the stagger meter much much faster once he's down. Attack the first round with autobattle as a Rav. The setup you want to use at first is Rav-Com-Med. Then the second round you will need to start AoO spam on the leg. After the first leg dies, switch to Sent/Med/Med, The Adamantoise will cast quake soon, so you will want to heal your party. Just wait for the quake and heal up then repeat the Army of One spam on the second leg until it falls. After he drops, make sure the debuffs are up on him before starting to stagger him. Then just use Rav/Rav/Rav until he staggers. Then switch to Com-Com-Rav and kill it before it stands back up. Fang: Fang will allow you to kill the legs much faster, and debuff him faster once he's down. After the buff and debuff has ended, you'll want to go to a Com-Rav-Rav and attack for one round. After that, switch to a Rav/Rav/Rav and stagger the leg. After it is staggered, attack it one more round in this paradigm. Then you will switch to a Com-Com-Rav. Attack it until it is about to destagger. Drop a Highwind on it at this point. This will kill the first leg. Switch to your Sent-Med-Med and heal you party. Then repeat the process for the second leg. If you are having trouble staying alive you can use a Rav-Rav-Med instead of Rav/Rav/Rav, but it will be slightly slower. After he drops, make sure the debuffs are up on him before starting to stagger him. Then just use Rav/Rav/Rav until he staggers. Then switch to Com-Com-Rav and kill it before it stands back up. The Pros - The fight comes down to whether you are strong enough or not. No more TP farming! The Cons - You must be very strong. -------------------------------------------------------------------------- 8. Frequently Asked Questions (FAQ) -------------------------------------------------------------------------- Here are where the Frequently Asked Questions will be put. Please look here before e-mailing me a question. If your question if not listed here, feel free to e-mail me. -------------------------------------------------------------------------- 8.01 Farming CP before Gil? (CBG) -------------------------------------------------------------------------- Q: Should I farm CP before I farm my Gil? A: No. Never farm CP, you will max out all of your character's CP long before you max out your Gil needs. -------------------------------------------------------------------------- 8.02 Getting Catalogs? (GCT) -------------------------------------------------------------------------- Q: Where do I get Catalogs? A: You get the Collector's Catalog during mission 8. You can also buy one later in the game for 100k. You will get a second Collector's Catalog after completing tier 7 of the Tower. You can then upgrade one of them into a Connoisseur Catalog using a Minar Stone after you star it. -------------------------------------------------------------------------- 8.03 Stacking Catalogs? (SCT) -------------------------------------------------------------------------- Q: Do Catalogs stack? A: The same Catalog equipped multiple times will not stack, but different Catalogs, (Collector, Conniseur and Survivor), will. -------------------------------------------------------------------------- 8.04 New Farming Methods? (NFL) -------------------------------------------------------------------------- Q: I've heard another method is good, you didn't cover it, is it really good? A: I dont know, send me an email, and we can figure it out. It might be a spot I dont know about. -------------------------------------------------------------------------- 8.05 Farming TP? (FTP) -------------------------------------------------------------------------- Q: How can I farm TP faster? A: In Eden, the Adamantoise is close to a save point, a zone line, and has a lot of very weak soldiers around to regain TP on. -------------------------------------------------------------------------- 8.06 Stagger Gauge Resetting? (SGR) -------------------------------------------------------------------------- Q: Will the Stagger Gauge reset if the Summon leaves? A: No, It will only reset if you enter Gestalt mode. So if you let him fly away, the Stagger Gauge will not be reset. -------------------------------------------------------------------------- 8.07 Chapter 13 Farming? (13F) -------------------------------------------------------------------------- Q: Why will the Chapter 13 Farm stop spawning after you beat the game? A: The 2 paths that extend to the sides will disappear once you beat the game. The two main paths will remain, but for some reason they will only respawn when you take a side path. -------------------------------------------------------------------------- 8.07 Chapter 13 Farming? (13F) -------------------------------------------------------------------------- Q: What should I farm? I am at xxx point in the game. A: I suggest doing mission 24 as soon as you can, getting t2 weapons. Then moving along to chapter 13, farm here until you have 5mil Gil or so, The go back to Pulse and farm Tortoises. Upgrading your weapons everytime you get a Trap. -------------------------------------------------------------------------- 9. Credits/Copyright Information (CC1) -------------------------------------------------------------------------- If you have any questions, comments, concerns, suggestions or errors, don't be afraid to contact me at: jesta23 (at) yahoo.com This may be not be reproduced under any circumstances except for personal, private use. It may not be placed on any web site or otherwise distributed publicly without advance written permission. Use of this guide on any other web site or as a part of any public display is strictly prohibited, and a violation of copyright The following website has permission to host this guide: GameFAQs - http://www.gamefaqs.com Thanks to: Jesta - Information/Farming Methods marter - Proofreading/Formatting GTTFOS - Stating Mission 24 is beatable in 10 seconds using the Com-Com-Com formation Keldonis - Suggesting to not kill the pack of 4 Sacrifice Ce'ith, and instead just respawn the pack of three Gate of Destiny - Providing the strategy and video for the "Adamantortiose Summon, Sahz leader" farming method FrankBrodie - Telling me about upgrading the second collector's catalog izza20 - Stacking Catalogs information/Farming TP information trunks76140 - Farming TP information Nimmy - Summon/Death method tips Tomasz - For suggesting the Champions Bangle and Energy sash. For the Summon methods. Jakey1994 - For siggesting the Gian rings to defend against stomps. -------------------------------------------------------------------------- 10. Version History (VSN) -------------------------------------------------------------------------- Version 0.75 - March 30, 2010 - Posted on GameFAQs Version 0.80 - March 31, 2010 - Major Format Overhaul Version 0.90 - April 4, 2010 - Added Video/Image Links - Added Chocobo Farming Section Version 0.95 - April 6, 2010 - Added Post-Game Ci'eth Farming - General Cleanup
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try to unzip me, and see my eyes fleeing away from you
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To Tell or Not to Tell: Disclosing an AS or ASD Diagnosis


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Our lab is having a never-ending ELISA issue, our assays which were all previously working beautifully and giving consistent results have stopped working right after we returned from holiday shut-down, as in no color change or very little color will develop along with a loss of proportionality. Here's our protocol:
Incubate overnight @ 4C in 0.2M sodium carbonate, 0.35M sodium bicarbonate, 0.03M sodium azide pH 9.6
Block with 1% BSA in PBS with 0.02% azide for 1 hr at RT while rocking
Incubate overnight @ 4C with primary in diluent (PBS, 0.05% Tween, 0.1% BSA, 0.02% azide)
Wash 5x in wash buffer (PBS, 0.05% Tween, 0.02% azide) and blot plate after each wash
Incubate 1 hr @ RT in secondary (alkaline phosphatase conjugated)
Wash 5x, then develop in KPL BluePhos substrate (BCIP based)
All assays have stopped working regardless of which primary antibody was used, and here's the list of everything we have done to try and fix the problem which we now believe may have to do with our water source (river water + spring runoff = bad???):
1) tried different lots of the binding plate (Corning high-binding)
2) had our water purification system serviced - new reverse osmosis membrane and thorough cleaning of the system and reservoir tank
3) purchased new coating buffer chemicals
4) purchased pre-made coating buffer capsules from Sigma, 0.05M carbonate and bicarbonate
5) purchased distilled water from the local CVS
6) tested all protein lysates and antibodies in westerns and dot blots
7) tried binding in PBS
Nothing has worked completely, some of our primaries will give a bit of decent color (~60% of former OD values) but we just can't find that magical combination that gets us back to where we were with all of our primaries.
Our coating buffer used to come out right at pH 9.6 without any adjustment, but now after our water service visit the pH starts around 10.1 to 10.2. We have brought aliquots of coating buffer back down to 9.6 with HCl, but it seems like we lose some carbon as CO2 gas during the process. If anyone has experienced something similar or has any suggestions please help, we're really at our wit's end and we're trying to graduate two students who need ELISA data for their dissertation projects. Thanks in advance for reading this and for any suggestions!
Lab ratty
Submit your paper to J Biol Methods today!
Previously great ELISAs have stopped working
Started by lab ratty, Mar 16 2010 08:17 AM
5 replies to this topic
Posted 16 March 2010 - 08:17 AM
Posted 16 March 2010 - 11:44 PM
First question, did you test the secondary with the substrate as you haven't it in your list and is it working?
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 17 March 2010 - 02:23 AM
You said "all previously working" there has to be one common reagent between them...the conjugate is a very good place to begin looking...if it is common to all the assays you describe.
Posted 18 March 2010 - 06:33 PM
Hi Gerard and sgt4boston, thanks for your suggestions. We have tested our conjugate, our AP-conjugated secondary is the same detection method we use for our Westerns, all of which are consistently nice so it seems like we're ok in that respect. A few thoughts we have had recently is that we don't have direct evidence of a lack of binding to the plates, it's more of an assumption, so we'll do a quick Sypro stain and see if anything sticks (and whether there is proportionality), and we had also considered using TBS-based buffers in place of the PBS since our Western protocol is so very similar to the ELISA and the Westerns are all in good shape. Not sure what a reviewer would say about TBS, but we're willing to try just about anything at this point!
Lab ratty
Lab ratty
Posted 19 March 2010 - 01:27 AM
Did you make fresh coating buffer, the pH change by different water isn't logical it's a buffer and no pH change is to be expected.
Did you check the pH of the substrate? And a question as I don't know the KPL BluePhos substrate, can the azide be a problem? Wrong made, 0.2% azide in the wash buffer.
Did you check the pH of the substrate? And a question as I don't know the KPL BluePhos substrate, can the azide be a problem? Wrong made, 0.2% azide in the wash buffer.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 21 March 2010 - 04:14 PM
Yes, we have remade coating buffer numerous times. The BluePhos is something we have used for years and it has never been impaired by the presence of azide in our other buffers, plus the ELISAs stopped working halfway through the batch of the BluePhos we were using at the time so that probably isn't it (didn't check pH but since it is a commercial product the pH is something that would be checked by Qual Control before it leaves the supplier, so I'm not sure we could get much info out of the pH, plus we don't know what the pH was before since we've never measured it). After the problems started we remade every single solution and double-checked our measurements and dilutions of chemicals including the azide solution, and it made no difference to our results. Buying pre-coated plates just isn't worth the money, so at the moment we're pretty well stuck. We did recently have an idea regarding how we dilute samples prior to coating the plate; we usually make all of our dilutions in the coating buffer directly, but we saw a product from a company to be used for diluting samples prior to coating which is supposed to enhance adsorption of antigen to the plate and stabilize the tertiary structure (ImmunoChemisrty Technologies 5x antigen coating buffer). Anyone have any experience with commercial diluting buffers?


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Photo of Carlos Hernandez Gomez from Fest For Beatles Fans Facebook page.
I can't claim to have known Carlos Hernandez Gomez, but those who did paint quite an affectionate portrait of him. Gomez, a political reporter for WBEZ radio as well as for the local television station CLTV in Chicago, passed away from cancer at age 36 this past January. Chicago Sun-Times columnist Carol Marin called him, "streetwise and smart, punky yet sweet."
He also made numerous friends among musicians in the city, and loved hanging out at Fest For Beatles Fans when it came to the Hyatt Regency in Rosemont each August. A post on the Facebook page for Fest For Beatles Fans notes that he was one of the people who sang "under the stairs." This is an area in the hotel's lobby where anyone at The Fest can join in on the communal spirit of singing Beatle songs. Terri Hemmert, the host of the weekly Breakfast With The Beatles show on WXRT, and a longtime emcee at The Fest, recalled Gomez as a friend, in a recent blog post on the station's website.
On Thursday, April 8th, there will be a special Concert For Carlos, with a multi-media slide show, live bands, and an auction featuring donations from Pete Townshend, Brian Wilson, Ray Davies, and other well-known musicians. Performers will include The Gear; Eric Howell; The Beatle Brothers; Phil Angotti; and special guest Brad Elvis. The show starts at 7PM.
It will take place at Fitzgerald's Night Club, at 6615 Roosevelt Road, in Berwyn. Tickets are $25 in advance, $30 at the door. All proceeds go to Living Water International Haiti, a charity Gomez was actively involved with up until his death. There is also an online auction. For more information, see fitzgeraldsnightclub.com and www.water.cc/carlos


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Boutique hotel experts Mr & Mrs Smith (www.mrandmrssmith.com) suggest 10 of the best getaways for unwinding in style, from relaxing rural retreats to snow-blessed mountain hideaways and simple seaside sanctuaries.
- Wilderness wonders
Wolgan Valley Resort & Spa, Blue Mountains, Australia
Just a scenic three-hour drive from Sydney, all-inclusive Wolgan Valley Resort & Spa in the Blue Mountains offers an iconic Australian country experience. Set in 4,000 acres of jaw-dropping, pristine wilderness, taking in vast valleys and elevated escarpments, it delivers dramatic nature in spades. Guided horse-riding and mountain-biking tours beckon, alongside wildlife safaris to spy kangaroos, wallaroos and platypuses. There's no need to sacrifice creature comforts though, with 40 pampering pool suites, fine-dining, a huge pool, tennis court and indulgent spa. Charles Darwin fell for Wolgan Valley in the 19th century, and it's easy to see why. (here)
2. Private-island chilling
Tikehau Pearl Beach Resort, Tuamotus, French Polynesia
What do you get when you cross France with the South Pacific? French Polynesia, a dreamy cluster of lagoon-lapped archipelagos in the deep-blue sea, boasting romantic islands and awe-inspiring coral atolls. The Tuamotus archipelago is one of the latter, playing host to tropical Tikehau Pearl Beach Resort hotel on an intimate motu (or mini isle). With a pretty pink-sand beach, bird life galore and a mix of 37 overwater and beachside bungalows looking over the lagoon, this is a tranquil, just-remote-enough paradise. It's perfect for a laid-back honeymoon or family escape, far from the madding crowds. Expect stellar snorkelling and diving. (here)
- Natural highs
Vigilius Mountain Resort, South Tyrol, Italy
If you prefer to head for the hills, ideally enveloped in an icing-thick layer of snow, then Vigilius Mountain Resort in north-east Italy's spectacular Dolomites is for you. Modern, minimalist swathes of wood and glass dominate the eco-sleek lodge, with 35 rooms and six suites, all mercifully free of Alpine kitsch. Spend your days skiing, snowboarding or Nordic walking, then hit the toasty spa. In summer, hiking, horse-riding, archery and mountain-biking are the big draws. Worked up an appetite? Guests can enjoy modern European fare at Restaurant 1500 or hearty stews and strudels at Parlour Ida. (here)
- Desert chic
Amangiri, Lake Powell, United States
When only a desertscape will do, escape to Amangiri boutique hotel near Lake Powell in the heart of Navajo country in southern Utah. An exclusive pocket of modernist luxury surrounded by wild and wind-carved land, this is rockstar chic at its best. The 34 soothing suites embrace the outdoors with stellar views, as does the sandstone-set outdoor heated pool. The incredible spa sports five treatment rooms, a watsu pool and Water Pavilion, and rates include yoga classes and a daily guided hike. Delicious food and drink takes inspiration from the region (think bison burgers, tortilla chips and salsa, and prickly-pear margaritas). (here)
- Spa serenity
MesaStila, Yogyakarta, Indonesia
If you fancy a jungle spa stay away from the tourist hordes, MesaStila hotel on Indonesia's main island Java could be just the ticket. Ringed by verdant forest and eight easy-on-the-eye volcanoes, this serene garden-set retreat was once a Dutch colonial coffee plantation. Now its 22 elegant villas promise to calm you down rather than rev you up, thanks to the award-winning spa, which uses estate-grown products in its treatments, a hammam, yoga classes and view-blessed pool. The culture-rich town of Yogyakarta is nearby for a hit of dance, art or music, and famous Buddhist temple Borobudur is also within easy reach. (here)
- Vineyard-hopping
Château de Bagnols, Beaujolais, France
For a rural retreat that knows a thing or two about wine, hightail it to luxurious Château de Bagnols, a five-star aristocrat near Lyon in cultivated Beaujolais. Boasting its own moat, drawbridge and towers in honey-hued stone, the hotel's 21 spacious rooms are elegant feasts of vintage style. Gourmet delights are also on offer at La Salle des Gardes restaurant, occupying a former guards' room beside a sunny terrace, which dishes up modern French cuisine as well as trad spit-roasted meats and game. There's an impressive wine list, and wine-tasting and cooking classes on tap. Horse-drawn carriage jaunts, picnics and hot-air balloon rides up the romance factor. (here)
- Modern-rustic luxe
Blackberry Farm, Smoky Mountains, United States
Gorgeous views, a patchwork of picket-fenced fields, sylvanian countryside, and a model home-on-the-farm feel – all the ingredients are there for a textbook rural retreat, but 59-room Blackberry Farm, in Tennessee's Smoky Mountains, ratchets the luxury up a notch with smart contemporary decor, can't-do-enough staff and its own Lexus fleet (or commandeer a golf buggy). Our fave rooms are the Singing Brook Cottages, which are superbly spacious, pleasantly secluded, and offer a gorgeous contemporary take on chic rusticity. The hotel flaunts outdoor pursuits aplenty, including hiking, climbing, rafting, riding and stargazing. The Aveda Spa offers an indulgent alternative for working off those all-inclusive meals. (here)
- Animal magic
The Farm at Cape Kidnappers, Hawke's Bay, New Zealand
The Farm at Cape Kidnappers in wine region Hawke's Bay, on New Zealand's North Island, combines a 6,000-acre working sheep and cattle farm with jaw-dropping Pacific Ocean views, an 18-hole golf course and soothing spa. Touting 22 suites (and the larger Owner's Cottage for group getaways), this boutique retreat offers serious luxe, fine dining and a romantic circular snug for James Bond-glam drinks. To get your fresh-air fix, opt for a farm tour by foot or four-wheel drive, go on a Kiwi Discovery Walk to spot the rare national bird or take a safari to the local gannet colony. (here)
- Country pursuits
The Wheatsheaf Inn, Cotswolds, United Kingdom
Just a two-hour drive from London, the Wheatsheaf Inn brings the trad English coaching inn bang up to date, with stylish design, tasty regional food and beer, and a small spa. Once weary wayfarers called into this 17th-century bolthole to recuperate. Now you can do the same, provided Cotswolds-loving supermodel Kate Moss and her posse haven't booked it out first. The 14 cosy suites include a former piggery and stableblock, but tasteful mod cons rule now. The dining room and bar are equally welcoming, teaming tempting dishes with bold modern art. Should you fancy yourself as an English country squire, try out clay-pigeon shooting, with Hunter wellies provided. (here)
- Jungle thrills
El Silencio Lodge & Spa, Costa Rica
Eco suites on stilts meet the cloud-forest canopy at El Silencio Lodge & Spa in central Costa Rica, set in a 500-acre tropical forest with a winding river and three waterfalls cascading down the mountainside. Take in picture-perfect panoramas from an outdoor hot tub, greet the sunrise from the yoga deck, and feast on natural, organic food (restaurant Los Ventanas uses produce from the garden and local fishermen pitch in with fresh trout). Each of the 15 mountainside Suites is assigned an Eco Concierge, who leads your courtesy hike, coordinates activities, arranges tree-plantings and can point out flora and fauna (expect beautiful birdlife in this private nature reserve). (here) (Editing by Paul Casciato)/