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CAIRO — Egypt's civilian cabinet offered to resign Monday after three days of violent clashes between demonstrators and security forces in Tahrir Square, but the action failed to satisfy protesters deeply frustrated with the new military rulers.
Earlier on Monday, Cairo police fought protesters demanding an end to army rule for a third day and morgue officials told Reuters the death toll had risen to 33, making it the worst spasm of violence since the uprising that toppled President Hosni Mubarak.
Egypt's ruling military council accepted the cabinet's resignation, Al-Jazeera television reported, citing unnamed sources. That report has not been confirmed by other news organizations.
Throughout the day, young protesters demanding the military hand over power to a civilian government fought with black-clad police, hurling stones and firebombs and throwing back the tear gas canisters being fired by police into the square, which was the epicenter of the movement that ousted authoritarian leader Hosni Mubarak.
Tens of thousands in square
By midnight tens of thousands of protesters were in the huge downtown square.
The clashes have deepened the disarray among Egypt's political ranks, with the powerful Muslim Brotherhood balking at joining in the demonstrations, fearing that turmoil will disrupt elections next week that the Islamists expect to dominate.
The protests in Tahrir and elsewhere across this nation of some 85 million people have forced the ruling military council as well as the cabinet it backs into two concessions, but neither were significant enough to send anyone home.
The council issued an anti-graft law that bans anyone convicted of corruption from running for office or holding a government post, a move that is likely to stop senior members from the Mubarak regime from running for public office.
Hours later, the cabinet of Prime Minister Essam Sharaf submitted its resignation to the council, a move that was widely expected given the government's perceived inefficiency and its almost complete subordination to the generals.
Protesters cheered and shouted "God is great!" when the news arrived of the Cabinet resignation offer, but they almost immediately resumed their chant of "The people want to topple the field marshal" — a reference to military ruler Field Marshal Hussein Tantawi.
"We are not clearing the square until there is a national salvation government that is representative and has full responsibility," said activist Rami Shaat, who was at the site.
The council released a statement late Monday calling for a national dialogue to "urgently study the reasons for the current crisis and ways to overcome it."
The statement, carried by Egypt's state news agency, said the military deeply regrets the loss of life and has ordered the Justice Ministry to form a committee to investigate the incidents of the past few days. The military said it ordered security forces to take measures that would protect demonstrators, who have the right to peaceful protest.
White House spokesman Jay Carney said the United States was deeply concerned about the violence and urged restraint on all sides so Egypt could proceed with a timely transition to democracy.
Election next week
The bloodshed in and around Cairo's Tahrir Square, epicenter of the anti-Mubarak revolt, threatens to disrupt Egypt's first free parliamentary election in decades, due to start next week.
Clashes have raged on and off since police used batons and tear gas to try to disperse a sit-in in Tahrir on Saturday.
Demonstrations were also taking place in Alexandria, Ismailia, Suez and Al Arish in Sinai, NBC News' Richard Engel reported from Cairo on Monday. Protesters vowed to organize a "million man" march in Cairo on Tuesday.
Protesters have brandished bullet casings in the square, but police deny using live fire. Medical sources at Cairo's main morgue said 33 corpses had been received there since Saturday, most of them with bullet wounds. At least 1,250 people have been wounded, a Health Ministry source said.
"I've seen the police beat women my mother's age. I want military rule to end," said 21-year-old Mohamed Gamal. "I will just go home in the evening to change my clothes and return."
Islamists dominated demonstrations against army rule on Friday, but the unrest in Tahrir since then has drawn in many of the young activists who helped topple Mubarak on February 11.
Army generals were feted for their part in easing him out, but hostility to their rule has hardened since, especially over attempts to set new constitutional principles that would keep the military permanently beyond civilian control.
Police attacked a makeshift hospital in the square after dawn on Monday but were driven back by protesters hurling chunks of concrete from smashed pavements, witnesses said.
"Don't go out there, you'll end up martyrs like the others," protesters told people emerging from a metro station at Tahrir Square, where about 4,000 had gathered by midday.Video: Violence returns to Egypt's Tahrir Square (on this page)
'No going back'
"There is clearly no going back as you can see this violence cannot be swept under the table," said Essam Gouda, a protester in Tahrir, who said two marches were due to converge there by mid-afternoon.
"We aim to control the entry points to the square so that security doesn't block protesters from entering," said Essam.Story: Egypt in uproar after blogger posts nude photos
The violence casts a pall over the first round of voting in Egypt's staggered and complex election process, which starts on November 28 in Cairo and elsewhere. The army says the polls will go ahead, but the unrest could deter voters in the capital.
Some Egyptians, including Islamists who expect to do well in the vote, say the ruling army council may be stirring insecurity to prolong its rule, a charge the military denies.
Political uncertainty has gripped Egypt since Mubarak's fall, while sectarian clashes, labor unrest, gas pipeline sabotage and a gaping absence of tourists have paralyzed the economy and prompted a widespread yearning for stability.
In Washington, White House spokesman Jay Carney said on Monday that the U.S. is deeply concerned about the violence and is calling for restraint on all sides. He said despite the clashes between security forces and protesters, Egypt must proceed with a timely transition to democracy.
Egypt's state news agency MENA said 63 flights to and from Cairo had been canceled because of the latest unrest.
The military plans to keep its presidential powers until a new constitution is drawn up and a president is elected in late 2012 or early 2013. Protesters want a much swifter transition.
The army said on Monday it had intervened in central Cairo to protect the Interior Ministry, not to clear demonstrators from nearby Tahrir Square, whom it also offered to protect.
"The protesters have a right to protest, but we must stand between them and the Interior Ministry," said General Saeed Abbas. "The armed forces will continue in their plans for parliamentary elections and securing the vote."
The Interior Ministry, in charge of a police force widely hated for its heavy-handed tactics in the anti-Mubarak revolt, has been a target for protesters demanding police reform.
"Unfortunately the Interior Ministry still deals with protests with the same security mentality as during Mubarak's administration," said military analyst Safwat Zayaat.
The latest street clashes show the depth of frustration, at least in Cairo and some other cities, at the pace of change.
"Military rule is defunct, defunct," crowds chanted. "Freedom, freedom."
Internet clips, which could not be verified, showed police beating protesters with sticks, pulling them by the hair and, in one case, dumping what looked like a body on a rubbish heap.
Residents reacted angrily when police fired tear gas into a crowd gathered below a burning building 200 meters (yards) from Tahrir Square, hindering the rescue of trapped residents.
Outside the burning apartment building, protesters chanted "Tantawi burned it and here are the revolutionaries," referring to Field Marshal Mohamed Hussein Tantawi, Mubarak's defense minister for two decades and leader of the army council.
"I don't want Tantawi ... I am staying tonight," said Ayman Ramadan, a data entry clerk, said early on Monday morning.
Doctors in orange vests were treating casualties on pavements in the middle of Tahrir.
Presidential candidate Hazem Salah Abu Ismail, a Salafi Islamist, told protesters: "We are demanding as the minimum that power be handed over within six months."
Presidential hopefuls Mohamed ElBaradei and Abdallah al-Ashaal denounced violence against protesters and called for a national salvation government, MENA said.
Liberal groups are dismayed by the military trials of thousands of civilians and the army's failure to scrap a hated emergency law. Islamists eyeing a strong showing in the next parliament suspect the army wants to curtail their influence.
Analysts say Islamists could win 40 percent of assembly seats, with a big portion going to the Muslim Brotherhood.
Reuters and the Associated Press contributed to this report


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Monday, April 22, 2013
SSiS Update
So we've had some life interfering in my crafting schedule LOL
Nonetheless I have made some progress and some decisions. I found my knit yardage, picked fabric for several things, prewashed the stuff that I thought needed it, and starched the knits.
Yes. Starched. With an iron. I picked that tip up from Ashley of Lil Blue Boo when I bought some of her patterns a couple of years ago. It REALLY helps when you are trying to cut pieces out of knit scraps to be cutting starched fabric.
So I starched several t-shirt scraps which, along with the black knit yardage I bought for myself like 2 years ago and a large chunk of navy blue knit yardage I don't recall having but somehow have, will make K-Ro a navy maxi dress with coral accents, Bubba a navy short set with green accents, a navy t-shirt to go with some navy gingham shorts, and a couple of black t-shirts to be stenciled. Because I'm a glutton for punishment and am gonna attempt freezer paper stenciling again after the last miserable failure...
I also found the missing peach fabric and striped tee that I KNEW I had. See. I KNEW I had it LOL
As for wovens, I found a bright striped seersucker that I had forgotten about... I'm going to make an 8 panel skirt out of it, cut in such a way to make chevrons, and either pair it with or attach it to a black t-shirt. I also discovered the purple striped fabric from the last post will NOT work for the Purpledicular dress. After washing the texture changed dramatically. I am SO glad I prewashed it though. Think of how mad I would have been if I sewed it all up and THEN washed it and had the stripes all drawing up on themselves...
Now I'm off to my craft room again. I have to decide if I want to go ahead and start cutting (and thus counting) tonight. We have a full day of errands tomorrow, but I'm impatient LOL
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Bobby and I partnered with Kenneth Cole, MTV International's Staying Alive Foundation and amfAR to spread awareness about AIDS with both my and the next generation. Kenneth Cole designed this special double-looped ribbon to symbolize coming together and doubling the efforts to fight HIV and AIDS, which was discovered thirty years ago. There isn't a cure yet (Charlene, I'm counting on you!), but it can be prevented. Educate and protect yourself.
Purchase your ribbon here to show your support for the cause. See more info at mtvcometogether.com.
From the release: The ribbon will be sold through December 31 and 100% of net profits go to AWEARNESS, the Kenneth Cole Foundation in support of the MTV Staying Alive Foundation and amfAR, The Foundation for AIDS Research, at Kenneth Cole retail and outlet stores in the U.S., Latin America, Israel, the Philippines and Kennethcole.com; Bloomingdale's nationwide and Bloomingdales.com; Gilt Groupe; and House of Fraser in the UK.


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Android gamers, here is some good news for you. One of the most anticipated games for the Android platform, Shadowgun finally landed in the Android Market a couple of days ago. The game comes from the developer of Samurai II – Madfinger Games – whose name is associated with quality games.
In Shadowgun, users play the role of John Slade, who is the Galaxy's most infamous bounty hunter. The role of John's (user's) is to hunt down and kill Dr. Edgar Simon. The game has amazing graphics, probably among the best you will ever see in an Android phone.
The sad part is that the game only supports Tegra 2 devices for now. Non-Tegra 2 handset owners need to wait for a few more weeks, until Madfinger releases a version that is compatible with all other Android devices. Here is the market link of Shadowgun.
In other gaming related news, Gameloft has released Tintin HD on the Android Market as well. As the name suggest, the game is based on the recently released movie, based on Tintin. The game has a typical Gameloft feel to it, and like most other games from the company, the controls of the game are pretty tough. The graphics of the game is also like any other Gameloft game.
The game is not yet available in all regions of the world, so users need to wait until Gameloft releases the game worldwide. Alternatively, they can buy the game from Gameloft's website as well.
In some Android app news, two popular apps – Winamp and 360 – also got some major updates. The new version of 360 brings a portrait mode, and a higher resolution panorama mode, along with a high quality and normal quality mode. The upload speeds of Panorama have also been decreased, along with the usual stability improvements. 360 for Android can be downloaded from here.
The new updated version of Winamp for Android brings quite a lot of new features including syncing with Winamp for Mac, and touch sensitive album art gesturing.
With this app, Winamp also gets an in-app Pro version worth $4.99. The pro version will give users access to a 10-band graphic EQ, customizable home screen, FLAC support, Reply gain, and much more. Winamp for Android can be downloaded from here.


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Tharo Systems Inc.'s H-600 Series 6-inch-wide thermal transfer barcode label printers, offered in 203 DPI and 300 DPI versions, feature a durable, all-metal construction with a cast-aluminum center wall for added strength. The company says that the printers also feature:
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Cell Export - Exocytosis
- 1 Introduction
- 2 Objectives
- 3 History
- 4 Looking in the Cytoplasm
- 5 The Cytosol
- 6 Compartments are Dynamic
- 7 Ribonucleic Acid (RNA)
- 8 Ribosomes
- 9 Endoplasmic Reticulum
- 10 Rough Endoplasmic Reticulum
- 11 Smooth Endoplasmic Reticulum (SER)
- 12 Transport Vesicles
- 13 Golgi Apparatus
- 14 Secretory Vesicles
- 15 Abnormalities
- 16 References
- 17 Cell Structure Images
- 18 JCB Movies
- 19 2016 Course Content
|This lecture introduces how information is transferred from stable stored information (DNA) converted to an intermediate (mRNA, rRNA, tRNA) of variable stability, exported from the nucleus to the cytoplasm where mRNA is then translated into Protein. This is gene expression, the products of this process are used either within the cell, exported (exocytosis) or used to replace worn out components.
We will study this topic at the level of the cellular components and organelles involved in the process: ribosomes, endoplasmic reticulum, Golgi apparatus, vesicles (transport and secretory). Now watch the movie in this introduction.
Archive: 2015 | 2014 | 2013 | 2012 | 2012 Print version | 2010 | 2009
MH - note that archive content listed above will not match exactly current lecture structure but has been selected as having similar content.
- Brief understanding of gene expression
- Understanding structure and function of organelles and structures associated with protein export (exocytosis)
- ribosome
- endoplasmic reticulum
- vesicles - types and transport
- Golgi apparatus structure and function
- Brief understanding of transport between secretory compartments
- Brief understanding of membrane turnover
Below are some example historical research finding related to exocytosis from the JCB Archive.
1955 Ribosomes, or the particles of Palade George Palade identifies particulate components of the cytoplasm, known initially as the particles of Palade and later as ribosomes.
1956 Microsomes are the in vitro ER George Palade and Philip Siekevitz unite the fields of microscopy and fractionation in this work. They conclude that Albert Claude's biochemical fraction called microsomes are the in vitro version of the endoplasmic reticulum (ER) — a cytological feature first noted by Keith Porter.
1958 A pathway for secretion Radioactive proteins are followed after their synthesis as they progress towards their secretory fate; this allows the definition of not only trafficking pathways but of the organelles that lie along that pathway.
1966 Excess secretory products fuse with lysosomes Robert Smith and Marilyn Farquhar find that excess secretory granules are not stored but fuse with multivesicular bodies (MVBs) that then mature and fuse with lysosomes.
1975 Lost in translation: the signal hypothesis Günter Blobel and Bernhard Dobberstein use a Rube Goldberg concoction of mouse RNA, rabbit ribosomes, and dog ER to reconstruct cell biology's version of the ship in the bottle: how proteins a cell intends to secrete end up in the endoplasmic reticulum.
2009 The Nobel Prize in Chemistry 2009 awarded to Drs Venkatraman Ramakrishnan, Thomas A. Steitz and Ada E. Yonath "for studies of the structure and function of the ribosome".
Looking in the Cytoplasm
Difference between Prokaryotes and Eukaryotes
- Light microscope - histology, immunohistochemistry
- lacks details within cytoplasmic compartment
- Immunochemistry
- Organelle dyes
- Fluorescent tagged proteins
- Electron microscope
- shows the organelles and membrane structure
Links: MCB - The secretory pathway of protein synthesis and sorting.
The Cytosol
- Membrane bound compartment
- About 1/2 total cell volume
- Intermediary metabolism takes place in the cytosol
- Chemical biological reactions
- Degradation
- Synthesis
- Protein molecules
- cell has about 10 billion (1x1010)
- 10,000 to 20,000 different kinds
Compartments are Dynamic
- Membrane bound compartments change shape and size
- Related to cell cycle, differentiation, signaling
Links: Overview of sorting of nuclear-encoded proteins in eukaryotic cells | MBoC Table 12-3. Some Typical Signal Sequences
Ribonucleic Acid (RNA)
- 3 types of RNA
- Messenger RNA (mRNA) translated into protein by action of ribosomes
- Transfer RNA (tRNA) each tRNA is specific for a specific amino acid (anti-codon)
- Ribosomal RNA (rRNA) Forms the backbone of ribosome subunits
Messenger RNA (mRNA)
- processed in the nucleus
- biological molecule (polymer) with unstable (half-life)
- exported to the cytoplasm
- site of ribosome assembly
Transfer RNA (tRNA)
- small RNA molecules which each binds a specific amino acid (anti-codon) tRNA
- carries it to the ribosome for protein assembly
Ribosomal RNA (rRNA)
- provide framework for dozens of proteins involved in assembly of AA sequence into protein
- most abundant RNA in cells
- Usually characterized by sedimentation coefficient
- rRNA genes are located in nucleolus
- multiple copies of human ribosomal RNA genes (rDNA) are arranged as tandem repeat clusters on the middle of the short arms of chromosomes 13, 14, 15, 21, and 22. PMID: 3336775
Links: MCB - Overview of mRNA processing in eukaryotes | MCB - movie - Life Cycle of an mRNA
Ribosome Structure
- Ribosome biogenesis consumes up to 80% of the energy of the cell PMID 10806485
- All RNA components mRNA, rRNA and tRNA come together in this structure
- two ribosome types with identical structure
- different locations - free and membrane bound
- Free in cytoplasm
- Bound to endoplasmic reticulum
- Also located within mitochondria
Ribosome Function
- Protein Synthesis
- complexes where RNA sequences are converted to amino acid (aa) sequences
- Codons 3 NTPs = 1 AA
- AA incorporated at 20/sec
- average sized protein takes 20-60 seconds to assemble
- Synthesis from amino- to carboxy- terminal of protein
- many ribosomes can bind 1 mRNA (polyribosome)
- signal recognition particle (SRP) - ribonucleoprotein (protein-RNA complex) that recognizes and targets proteins to the endoplasmic reticulum
- polyribosomes or polysomes are the EM visible granules
- many ribosomes bound to a single mRNA
- single ribosome covers a 54bp mRNA region
- the synthesised single amino acid chain can then be "modified"
- in the cytoplasm or in specialised organelles
- Protein Modification/Function
Links: MBoC - Figure 1-10. A ribosome at work | MBoC - Figure 12-37. Free and membrane-bound ribosomes | MBoC - Figure 6-63. A comparison of the structures of procaryotic and eucaryotic ribosomes | MCB - Model of protein synthesis on circular polysomes and recycling of ribosomal subunits (movie)
Endoplasmic Reticulum
Links: Endoplasmic Reticulum during Drosophila Mitosis | MBOC - The Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Rough Endoplasmic Reticulum - Function
- Allows specific proteins to be modified and targeted to different destinations
- Modification
- amino acid chain cleaved or sidegroups added (mainly glycosylation)
- glycosylation = addition of carbohydrate (sugar) groups
- Destination
- Domestic - Cytosolic, Nuclear, Organelles
- Exported from cell
Links: MCB - Overview of sorting of nuclear-encoded proteins in eukaryotic cells | MCB - movie - Protein Sorting JCB- movie - Real-time video of the formation of tubules at ER export sites
Rough Endoplasmic Reticulum - Structure
- about 50% of cell membrane
- continuous with outer nuclear membrane
- single highly convoluted membrane enclosing a single space
- ER lumen = ER cisternae
- "rough" because of many ribosomes attached to the membrane
- ribosomes bound only to cytoplasmic side of ER membrane
Links: MBOC - The Endoplasmic Reticulum JCB - movie - Three-dimensional view of Sar1 tubules
Smooth Endoplasmic Reticulum (SER)
Smooth Endoplasmic Reticulum - Structure
- Part of same membrane as RER
- may also be called "transitional"
- no attached ribosomes
- not involved in protein synthesis
- differ in shape
- SER a meshwork of fine tubules
Smooth Endoplasmic Reticulum - Function
- lipid metabolism (membrane)
- carbohydrate metabolism
- detoxification of drugs and harmful compounds
- steroid synthesis and metabolism (cholesterol)
- different amounts in different cells
In muscle cells SER stores and releases calcium ions (Ca2+} to trigger muscle contractions.
(Movie: RER to Golgi)
Links: MBoC - Transport from the ER through the Golgi Apparatus
Transport Vesicles
- RER synthesized material is transferred by budding off of membrane
- Forms transport vesicle
- Transports substances to different cellular locations
- Most transport to Golgi apparatus
- Active transport mainly along microtubules (cytoskeleton)
Links: MBoC - Vesicular Traffic | JCB - movie - transport vesicles and lipid (large 9.7 Mb)
Golgi Apparatus
Golgi Apparatus - History
- Discovered over 100 years ago
- Camillo Golgi (1898)
- seen in neurons as anastomosing threads
- "internal reticular apparatus"
- Soon detected in many cells
- Nobel Prize1906 Camillo Golgi, Santiago Ramon y Cajal
Links: MBOC - Golgi Apparatus- Summary
Golgi Apparatus - Structure
- organelle, membrane enclosed structural compartment
- cell may contain one or more Golgi apparatus
- located near the nucleus
- disc shaped membrane stack with different regions by their location within the cell
Golgi stack
- from 6-30/stack
- 3-100s stacks/cell
- many sets of membrane bound smooth surfaced cisternae
Stack Nomenclature
- cis - bottom of stack closest to endoplasmic reticulum, receives transport vesicles from ER
- medial - middle of stack, processing of proteins, modification of sidechains
- trans - top of stack closest to plasma membrane, buds off secretory vesicles
Links: MBoC - Golgi Apparatus | MBoC - Figure 13-30. Two possible models explaining the organization of the Golgi apparatus and the transport of proteins from one cisterna to the next | MCB - Figure 5-49. Three-dimensional model of the Golgi
Golgi Apparatus - Functions
- Sorting of cytosolic/secreted proteins
- Glycosylation of secreted proteins
- Modification of carbohydrates
- Side chains are also trimmed
- Trans vesicles fuse with the plasma membrane
Golgi and centrosome during cell cycle
Secretory Vesicles
- protein export (secretion)
- constitutive and regulated
- related also to membrane turnover
- new lipid
- new cholesterol
- new membrane proteins
Vesicle Movies
Links: JCB - movie - Insulin Secretion (3.4 Mb) | JCB - movie - transport vesicles and lipid (9.7 Mb) |
- accumulation of abnormal proteins
- misdirected (wrong "postcode")
- truncated or altered modification (missing enzymes)
- Genes and Diseases
- OMIM - Online Mendelian Inheritance in Man http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim
Links: NCBI - Genes and Diseases | NCBI - OMIM |
Essential Cell Biology
- Chapter 14 Intracellular Compartments and Transport
Molecular Biology of the Cell
Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter New York and London: Garland Science; c2002
- Molecular Biology of the Cell 4th ed. - Part 1 Chapter 12
- Figure 13-57. Figure 13-1. The endocytic and biosynthetic-secretory pathways
- Figure 13-57. Exocytosis of secretory vesicles
- Figure 13-59. Electron micrographs of exocytosis in rat mast cells
Molecular Cell Biology
Lodish, Harvey; Berk, Arnold; Zipursky, S. Lawrence; Matsudaira, Paul; Baltimore, David; Darnell, James E. New York: W. H. Freeman & Co.; c1999
- Molecular Cell Biology - Chapter 17. Protein Sorting: Organelle Biogenesis and Protein Secretion
- The Dynamic Cell
- MCB - Model of protein synthesis on circular polysomes and recycling of ribosomal subunits
- MCB - movie ch4anim4.mov
The Cell- A Molecular Approach
Cooper, Geoffrey M. Sunderland (MA): Sinauer Associates, Inc.; c2000
- The Cell - A Molecular Approach - Chapter 9. Protein Sorting and Transport - The Endoplasmic Reticulum, Golgi Apparatus, and Lysosomes
- An Overview of Cells and Cell Research
Search Online Textbooks
- "exocytosis" Molecular Biology of the Cell | Molecular Cell Biology | The Cell- A molecular Approach | Bookshelf
- "ribosome" Molecular Biology of the Cell | Molecular Cell Biology | The Cell- A molecular Approach | Bookshelf
- "endoplasmic reticulum" Molecular Biology of the Cell | Molecular Cell Biology | The Cell- A molecular Approach | Bookshelf
- "golgi apparatus" Molecular Biology of the Cell | Molecular Cell Biology | The Cell- A molecular Approach | Bookshelf
- "secretory vesicle" Molecular Biology of the Cell | Molecular Cell Biology | The Cell- A molecular Approach | Bookshelf
- PubMed is a service of the U.S. National Library of Medicine that includes over 18 million citations from MEDLINE and other life science journals for biomedical articles back to 1948. PubMed includes links to full text articles and other related resources. PubMed
- PubMed Central (PMC) is a free digital archive of biomedical and life sciences journal literature at the U.S. National Institutes of Health (NIH) in the National Library of Medicine (NLM) allowing all users free access to the material in PubMed Central. PMC
- Online Mendelian Inheritance in Man (OMIM) is a comprehensive compendium of human genes and genetic phenotypes. The full-text, referenced overviews in OMIM contain information on all known mendelian disorders and over 12,000 genes. OMIM
- Entrez is the integrated, text-based search and retrieval system used at NCBI for the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, Taxonomy, and others Entrez
Search Pubmed
- "exocytosis" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "ribosome" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "endoplasmic reticulum" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "rough endoplasmic reticulum" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "smooth endoplasmic reticulum" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "golgi apparatus" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- "secretory vesicle" PubMed reviews | PubMed all articles | PMC reviews | PMC all articles | OMIM | Entrez all databases
- Lipid rafts and the regulation of exocytosis. Salaün C, James DJ, Chamberlain LH. Traffic. 2004 Apr;5(4):255-64. Review. PMID: 15030567
Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals. Zenisek D. Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4922-7. Epub 2008 Mar 13. PMID: 18339810
A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells. Ozawa T, Nishitani K, Sako Y, Umezawa Y. Nucleic Acids Res. 2005 Feb 24;33(4):e34.PMID: 15731327
Cell Structure Images
The linked pages below currently contain unlabeled electron micrographs showing specific cellular features.
- Nucleus Images
- Endoplasmic Reticulum Images
- Golgi Apparatus Images
- Molecular Probes -Organelles Golgi Apparatus and Endoplasmic Reticulum
JCB Movies
- A marker for sequential exocytosis The SNARE protein SNAP25, say Takahashi et al., marks the plasma membrane after an initial exocytic event to allow rapid sequential exocytic events.
- A myosin V moves yeast secretory vesicles Secretory vesicles actively move to the site of exocytosis in yeast. Schott et al. find that multiple secretory vesicles often follow the same linear track and frequently enter and cross the bud. This movement requires the activity of the myosin-V heavy chain encoded by the MYO2 gene. When the predicted lever arm of this motor is progressively shortened (with the most extreme example being the 0IQ mutant), the vesicle movements are progressively slowed.
- Rapid cycling of lipid rafts to and from the Golgi Nichols et al. detect rapid cycling of lipid raft markers between the plasma membrane and the Golgi. Through selective photobleaching, they are able to study transport either out from the Golgi to the plasma membrane, or in from the plasma membrane to the Golgi.
- Membrane docking at the immunological synapse requires Rab27a Stinchcombe et al. find that normal membrane docking of lytic granules at the immunological synapse is defective in cells lacking Rab27a. In cells lacking other Rab proteins, polarization of the secretory granules is incomplete.
- Visualizing the location and dynamics of exocytosis Schmoranzer et al. use total internal reflection (TIR) fluorescence microscopy to visualize exocytosis in mammalian cells (e.g., see event on left side of video). The analysis reveals that there are no preferred sites for constitutive exocytosis in this system.
- Visualizing the location and dynamics of exocytosis Toomre et al. use a combination of TIR microscopy (green, labeling molecules close to or at the membrane) and standard fluorescence microscopy (red, for molecules further from the membrane) to visualize [/content/vol149/issue1/images/data/33/DC1/Fig_1b.mov trafficking to and fusion with] the plasma membrane during exocytosis. Red dots turn yellow then green as they approach the membrane, and then explode in a burst of light as they fuse with the plasma membrane during exocytosis. The transport containers appear to be partially anchored at the membrane before fusion, and can undergo either partial or complete fusion events.
2016 Course Content
Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2016 Revision
Laboratories: Introduction to Lab | Microscopy Methods | Preparation/Fixation | Cell Knockout Methods | Cytoskeleton Exercise | Immunochemistry | Project Work | Confocal Microscopy | Tissue Culture | Stem Cells Lab | Microarray Visit
Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G


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Information on coronavirus. Agency service suspensions/reductions. Apply to be an Open Restaurant.Information on coronavirus
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September 27, 2010Announces Plans to Champion Innovation, Reward Excellence in Teaching, Raise Standards in the Classroom and Implement a Rating System for Tenure
Mayor Michael R. Bloomberg kicked off NBC's "Education Nation" summit today by announcing plans to prepare every New York City student for college and careers in an increasingly competitive global economy. To ensure that every child has access to an effective teacher, the City will use a $36 million Teacher Incentive Fund grant from the US Department of Education (USDOE) to enlist highly-skilled teachers to work in low-performing schools and mentor fellow instructors. New technology and strategies that help personalize learning for every child will lead to the creation of 400 Innovation Schools over the next three years. The City will also change the way it grants tenure, moving to a rating system that will ensure tenure is linked to classroom performance. The City will also launch new partnerships with the private sector, including one with IBM and the City University of New York. Working together, the partnership will open a new school that runs from grades 9 through 14, allowing students to earn an Associate's Degree and be first in line for a job at IBM. The City will also create a joint task force, supported by a $3 million award from the Gates Foundation, to focus on aligning standards between the City's K-12 public schools and community colleges in an effort to double college completion rates by the end of the decade.
"Each and every one of us has a role to play in taking an education system that has fallen far behind the times, and moving it into the 21st century," said Mayor Bloomberg. "New York City is stepping up to that challenge and laying the foundation to ensure that every child who graduates high school is ready to start college or a career. By rewarding teachers who make a real difference, bringing technology into our classrooms and creating partnerships with the private sector, we will build upon the improvements we have made over the last eight years and give New York City children the future they deserve."
Intensifying its focus on teacher quality and effectiveness, the City plans to redouble its efforts to place a high-quality teacher in every classroom. Last week, New York City received a $36 million grant as part of the USDOE's Teacher Incentive Fund (TIF) competition, which it will use to target struggling students by placing a cohort of "master teachers" and "turnaround teachers" in low-performing schools. The money will be directed toward added compensation for both types of instructors – an additional 30 percent salary bonus for master teachers, and 15 percent for turnaround teachers – who have demonstrated significant gains in student achievement. The City will also expand its "executive principal" program, deploying ten additional principals to improve outcomes for students in struggling schools.
The City will also implement a four-tier rating system for determining whether a teacher should be awarded the lifetime job protections that come with tenure. Beginning this year, only teachers rated "effective" or "highly effective" will be eligible for tenure. This will transform the tenure system from one in which tenure is taken for granted, to one in which it must be earned through effective performance in the classroom. Tenure may be awarded in the third year, or any time thereafter, always contingent on whether a teacher has made a significant impact on student achievement.
"The crisis in our schools is urgent, but not dire," said Chancellor Klein. "If we take bold steps – challenge traditional classroom models and hiring practices, raise standards and create a bridge to college and employment – then we can truly make a difference in our nation's future."
"Too many of our children are falling through the cracks and not getting the education they need," said Deputy Mayor Walcott. "By implementing a combination of public investments and partnerships with private foundations, the City will be able to advance bold reforms, redefine common practices in public education and give children the education they deserve."
To empower teachers to meet the needs of every individual child, the City has begun to pilot a range of innovations in 80 schools – in technology, time spent in the classroom, and instructional delivery. Forty schools will pilot a "virtual school" model that integrates online learning with face-to-face classroom instruction, allowing students to learn at their own pace. Seven schools will pilot innovations in the way schools use time and staffing, implementing new ways to extend the school year and increase time spent in the classroom. And, 30 schools will be introducing technology that helps teachers evaluate student progress in real time. By increasing the number of Innovation Schools to 400 over the next three years, New York City willfundamentally change the way teachers are able to support student learning. Rather than spending the day lecturing to a room full of students, teachers will be able to use technology to tailor assignments to students' learning styles and needs by working with them as individuals, in small teams, or on projects specifically designed for them.
In the midst of an increasingly competitive global economy, the City will also continue its move toward raising standards to prepare students for college or careers in the 21st century. In 2008, the City University of New York and the Department of Education, supported by a range of community organizations, initiated a partnership focused on raising standards, sharing data, and measuring the success of students who graduate from City high schools. Today, the Bill and Melinda Gates Foundation and the National League of Cities announced that they would recognize this partnership with a $3 million grant, making New York City one of four recipients of the foundation's Communities Learning in Partnership (CLIP) Implementation Grant; the other recipients are San Francisco and Riverside, California, and Mesa, Arizona. New York City will use the award to align academic standards between high schools and City community colleges; better coordinate academic counseling; and work to develop a common benchmark for measuring college readiness, which may be used in the City's accountability system for grading schools at all levels.
"We are deeply grateful to the Gates Foundation for its generous support of CUNY and its partners as we seek to prepare students for the extraordinary challenges of the 21st Century," said City University of New York Chancellor Matthew Goldstein. "All of us know that without proper preparation and support in grades K-12, followed by strong academic programs in college, earning a degree will be a challenge for any student. All K-16 educators must work together – consistently and openly – to reach every student. While we have forged a strong collaboration with the City Department of Education, the purpose of this grant is to further align our two systems, to insure that high school students, upon graduation, are college ready for college work. Only then can we maximize college success as defined by increased graduation rates. With the help of this generous grant, we are well on our way to not only enhance our partnership, but also to move closer to our goal of doubling the numbers of students graduating from all of our community colleges over the next ten years."
With the generous support of IBM, the Department of Education and City University of New York will also work jointly to develop a school that runs from grade 9 through the equivalent of grade 14. Students will receive instruction in the traditional core subjects, while also learning the basics of computer science. All students will have the opportunity to graduate from grade 14 with an Associate's Degree and possible job placement with IBM.
The City will also work with state legislators to remove antiquated laws that require schools to purchase printed textbooks and force students to study subject matter even after they have mastered the material.
Over the last 8 years, New York City's 1.1 million students have made progress. Graduation rates have gone up 16 percentage points, and our African American and Hispanic students have closed the ethnic achievement gap on state tests by 37 percent in reading and 18 percent in math. The proposals outlined today will look to improve student proficiency in grades K-12 and double the CUNY 4-year graduation rate by 2020.
Stu Loeser/Jessica Scaperotti (Mayor)
(212) 788-2958
Natalie Ravitz/Matt Mittenthal (Education)
(212) 312-3523
Michael Arena (CUNY)
(212) 794-5685


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U.S. companies restocked at a faster pace in January, a sign that businesses expect stronger job growth to fuel more sales.
The Commerce Department said Tuesday that business stockpiles rose 0.7 percent in January after a 0.6 percent gain in December. Sales increased 0.4 percent January following a 0.9 percent December sales gain.
The increase pushed stockpiles up to $1.57 trillion, 19.1 percent above the recession low hit in September 2009.
Companies are building up their stockpiles again after cutting them over the summer amid recession fears. Higher inventories require more production, which boosts economic growth. It also suggests companies expect more sales.
A separate Commerce report Tuesday showed Americans increased their spending on retail goods 1.1 percent in January, evidence that job gains are boosting the economy. Consumers bought more autos, clothes and appliances. They also paid higher prices for gas.
Rising inventories were a key reason growth accelerated in the final three months of last year. Some economists had predicted restocking would slow in the current quarter and weaken growth.
But more job gains could alter that view. The economy just completed the three best months of hiring in two years, adding 734,000 net jobs from December through February. That's lowered the unemployment rate to 8.3 percent.
A rebound in hiring helped boost consumer confidence in February to the highest level in a year. It is also fueling more consumer spending, which accounts for 70 percent of economic activity.
Rising sales are keeping stockpiles from getting too high. If companies feel their inventories are excessive, they could cut back on orders, slowing the economy.
In January, retail stockpiles rose 1.1 percent to lead all categories. Manufacturing inventories increased 0.6 percent, while wholesale inventories gained 0.4 percent.
The January sales increase was led by a 0.9 percent rise in manufacturing sales.
The changes left the ratio of inventories to sales at 1.27 in January. That means it would take just 1.27 months to exhaust existing inventories at the January sales pace. The inventory-to-sales ratio peaked near 1.5 months in early 2009 as the recession cut into demand and businesses were caught with unwanted stockpiles.
Steven Wood, chief economist at Insight Economics, said with sales continuing to rise along with inventories the inventory-to-sales ratio remained "relatively lean."
In the October-December quarter, the economy grew at an annual rate of 3 percent. Nearly two-thirds of the fourth quarter growth came from a surge in inventory rebuilding.
Economists at JPMorgan Chase forecast growth in January-March at an annual rate of 1.5 percent, largely because of the slower growth in restocking. For the whole year, they expect the economy will grow 2.2 percent, up from 1.7 percent growth in 2011.
Stockpiles held by manufacturers account for nearly 40 percent of total business inventories, while wholesalers and retailers each hold about one third.
— Martin Crutsinger ASSOCIATED PRESS


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Quincy Brown was sitting at home in Blue Island on the afternoon that three of Jennifer Hudson's family members were slain when he says he got a panicked call from his friend William Balfour.
"At the time he was speed talking. He was in a rush, like a panic," Brown, 30, testified today as the third week of testimony began at Balfour's trial in the murders of the singer's mother, brother and young nephew.
Balfour seemed desperate as he begged Brown to help him fix the power steering in his car, according to the testimony. Balfour wanted to meet at 71st Street and Vincennes Avenue almost immediately, even though he knew Brown was miles away in the south suburbs, Brown said.
While they were talking, Brown said, Balfour took another call. When he came back on the line, Balfour said his estranged wife, Julia Hudson, was with the police and asking why he killed her mother and brother.
"I said, 'Are you for real? Why would she blame you of all people?' " Brown testified. "And he said, 'Because I got into it with that (expletive),' " making a crude reference to his wife's brother, Jason Hudson.
After he hung up the phone, Brown said he saw the breaking news stories on TV that Hudson's mother, Darnell Donerson, 57, and brother, Jason Hudson, 29, had been found shot to death in the family's Englewood home. There was an Amber Alert for Hudson's missing 7-year-old nephew, Julian King, and Balfour was being named as a possible suspect.
Brown testified he decided to call Balfour back and tell him he was concerned. Brown said Balfour claimed he was "out west" and accused him of "acting like the police" when he kept asking questions. When Brown told Balfour he was all over the news, he acted surprised, Brown said.
"Then I asked him boldly, did he do it?" Brown testified. "He said, 'It's bigger than me,' and that's when he hung up the phone."
Brown, who is serving almost a 5-year prison sentence for possession of a weapon by a felon, wore an orange prison jumpsuit for his testimony. He denied that he was getting any special treatment or leniency for his cooperation. He also admitted he had not told his story to investigators until they visited him last year in the federal prison in Leavenworth, Kan.
Brown also testified that three days before the murders, he was with Balfour at a get-together when Balfour flew into a rant about Jason Hudson and how he "hated that (expletive)" before pulling out the .45-caliber pistol he'd allegedly stolen from Jason Hudson's room and slamming it on a card table.
The next day, Brown said, he was driving Balfour's car and saw the gun in the center console. On cross-examination, Brown admitted he had fired the gun for fun that day from his friend's porch and then driven Balfour's car afterward.
Last week, a forensic expert had testified he found gunshot residue on the steering wheel cover of Balfour's car.
Balfour, 30, is accused of bursting into the Hudson family home on Oct. 24, 2008, and killing Donerson and Jason Hudson, then kidnapping and killing Julian before leaving the boy's body on the West Side in Jason Hudson's stolen SUV. Prosecutors alleged Balfour was upset his estranged wife wanted a divorce and was seeing another man.
Twitter: @jmetr22b


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This song features pummeling rhythm guitar and a tricky solo. Make sure to check out Dennis' "Tricks" lesson in the Lead Concepts and Technique series, as a few of the techniques explained there are used in this lesson.
Taught by Dennis Hodges in Metal Songs with Dennis seriesLength: 35:47Difficulty: 2.5 of 5
very nice
thanks man
hey...isnt this a christian band? kinda seeme familiar on christian metal stations...maybe not....
yes, they are classified as a "Christian" band, I didn't mention it in the video because I didn't want to start a "thing" on here. The first time I heard it, I thought, "holy crap, there are some pretty Pantera-ish riffs in there," which is a good way to win me over. So, that's what sold me on it.
funny shirt
you make it sound bad that it's a "christian"band,and whats up with "i didn't whant to start a thing"?
I think bringing up religion in a guitar lesson is probably a bad idea because no matter what it will start a shit storm. I doubt Dennis had any reason other then trying to avoid an argument. You know how things are on the internet.
Ever watched anything on Youtube then looked at the comments section? Arguments break out all the time, especially when religion is brought into the picture. It doesn't even have to relate to the video at all, people just like to fight about stuff like that.
so true
Yeah it is, however metal is metal. Demon hunter is considered christian and they have amazing songs.
yeah,chrisian metal
By the way, Great riffs in there. Also good to see you getting into it, saw a bit of the metal come out, with the headbanging XD Rock on Dennis!
another great one Dennis!!!!
it's okay
plez could u do a song called seek and destroy by metalica ps u rule
sad but true is way better though
What happened to Mr. Crowley??? its not on the site anymore!
Gotta love this band! can we expect some Demon Hunter in the future?
I never heard of these guys before but this is a really fun song to play. Thanks Dennis!
cool shirt
oh man i just realizedi tabbed this by accident and told my singer i wrote it.....thats why it was so awsome
i love christian metal stuff..sweet
thanks for including some commentary on the time signatures and beats during the lesson. it helps reinforce those concepts from your previous lesson.
i cant believe there's actually a Showdown lesson one here! ive been a huge fan since theire first album, but ive never met anyone else who knows them! rock on Dennis! \m/
Learn to rock out to the heavy metal classics throughout history.
Lesson 2
Dennis Hodges teaches "Beast and the Harlot" by Avenged Sevenfold.Length: 59:00 Difficulty: 3.0 Members Only
Lesson 4
Dennis brings you "Toxicity" off of the 2002 album from System of a Down. Get your metal face ready.Length: 29:22 Difficulty: 2.5 Members Only
Lesson 5
Learn "Laid to Rest" by Lamb of God. Released in 2004, this action-packed tune is the first track on their third album, Ashes of the Wake.Length: 25:34 Difficulty: 3.5 Members Only
Lesson 6
"Symphony of Destruction" is track #2 on Countdown to Extinction. This tune is a Megadeth fan favorite.Length: 26:43 Difficulty: 3.5 Members Only
Lesson 7
Off their 1992 album Vulgar Display of Power, this Pantera song redefined "respect".Length: 32:46 Difficulty: 2.0 Members Only
Lesson 11
"Inside the Fire" is the first single from Disturbed's fourth studio album, Indestructible. Dennis teaches all parts of this song including the solo!Length: 33:28 Difficulty: 2.5 Members Only
Lesson 14
Dennis Hodges teaches Bullet For My Valentine's "Waking The Demon" off their Scream Aim Fire album.Length: 41:54 Difficulty: 3.5 Members Only
Lesson 16
This is the title song from Cannibal Corpse' first EP. Released in 1993, this is the bands most popular song mainly due to an appearance in Ace Ventura: Pet Detective.Length: 33:05 Difficulty: 2.5 Members Only
Lesson 17
Dennis Hodges shows you how to play this Judas Priest classic, which is actually a cover of a Fleetwood Mac song.Length: 30:06 Difficulty: 2.5 Members Only
Lesson 18
Released in 2008 on The Showdown's third record, Back Breaker. This song features pummeling rhythm guitar and a tricky solo.Length: 35:47 Difficulty: 2.5 Members Only
Lesson 19
Learn how to play "(sic)" by Slipknot in this lesson.Length: 20:12 Difficulty: 1.5 Members Only
Lesson 20
Dennis puts a heavy metal spin on this classic Christmas song.Length: 18:04 Difficulty: 1.5 Members Only
Lesson 21
Dennis Hodges teaches this pummeling metal song from the band Meshuggah.Length: 56:12 Difficulty: 4.5 Members Only
Lesson 23
Learn this great Megadeth song from their album Cryptic Writings.Length: 39:39 Difficulty: 3.0 Members Only
Lesson 24
Learn this Megadeth classic from Rust In Peace.Length: 53:39 Difficulty: 4.0 Members Only
Lesson 25
Learn this Megadeth classic from the Countdown To Extinction album.Length: 36:16 Difficulty: 2.0 Members Only
Lesson 26
Learn this great ballad from the Youthanasia record.Length: 36:32 Difficulty: 3.5 Members Only
Lesson 27
Dennis Hodges teaches this Metallica classic from the band's 1988 album ...And Justice For All.Length: 69:02 Difficulty: 3.5 Members Only
Lesson 28
In this lesson, you'll learn how to play "Quintessence" by progressive metal band, Mastodon. The song is on the critically acclaimed album Crack the Skye, which was released in 2009. Dennis Hodges...Length: 33:01 Difficulty: 3.0 Members Only
Lesson 29
Dennis Hodges teaches this Dokken classic note for note. From the signature riffs to the blistering solo, Dennis breaks it all down for you in this lesson.Length: 41:39 Difficulty: 3.0 Members Only
Lesson 30
Here is a lesson on this devastating piece of classical music written by Niccolò Paganini in the early 19th century. The lesson is aimed at shred guitarists because of the high level of technical proficiency...Length: 49:14 Difficulty: 4.5 Members Only
Lesson 31
Learn how to play "Cowboys From Hell" by Pantera, the song that made them a household name. The song has an instantly recognizable guitar riff and showcases what Pantera is all about: groove metal.Length: 40:00 Difficulty: 4.0 Members Only
Lesson 32
Here is a lesson from Dennis Hodges on Disturbed's most well known song, "Down With The Sickness". The song is a concert staple and the album was certified platinum. Dennis breaks down all the parts and...Length: 26:00 Difficulty: 2.0 Members Only
Lesson 33
Dennis Hodges teaches all the parts to this power ballad by heavy metal band Pantera. At over 7 minutes, this is the longest Pantera song ever recorded. This song has one of "Dimebag" Darrell Abbott's...Length: 65:30 Difficulty: 4.5 Members Only
Lesson 34
Learn one of Dimebag Darrell's most famous riffs with this lesson on "I'm Broken" from Far Beyond Driven. This song is a "must-learn" for metal students.Length: 31:00 Difficulty: 3.0 Members Only
Lesson 35
Channel your inner hate and turn it into something productive with this lesson on "Mouth For War" from the metal band Pantera. This song is from the record Vulgar Display of Power, which was released...Length: 30:00 Difficulty: 3.0 Members Only
Lesson 36
Learn "This Love" by the metal band Pantera. Released on Vulgar Display of Power in 1992, this ballad has both haunting clean parts and brutal distorted riffs.Length: 50:00 Difficulty: 3.0 Members Only
About Dennis Hodges
View Full Biography
For better or worse, Dennis Hodges cannot stop playing music, and (he hopes) will never stop playing music.
Growing up in Flint, Michigan, Dennis had a tremendous passion for drawing. He couldn't stop copying moves from bands he saw on MTV, though, and it didn't help that his parents filled the house with Santana, Stevie Ray, and Allman Bros. (on real records, no less!) so it wasn't long till he got his first guitar. It was junk. Within a few weeks his parents traded in a poor acoustic for a less junky 3/4-size electric.
Dennis started lessons right away at the age of 8. He still remembers hating it for awhile, and not taking it seriously until he was 12. He is thankful his parents forced him to practice early on and kept paying for lessons, even though rational thinking should have stopped them after a year.
Around this time drawing became less important, and guitar consumed all his attention. After 6 years of lessons he parted ways with his teacher and, after trying out two others with no results, decided to continue alone. His nerdistic tendencies paid off, as he put in hours working on picking and left hand exercises and learned as many Randy Rhoads and Kirk Hammett solos as he could.
Luckily, there were playing opportunities at school talent shows and church. Dennis was playing bass at his church when he was 13, helping to hone his performance skills in a group setting.
In high school, Dennis joined the marching band on sousaphone for all 4 years. It was as awesome as you could expect. He was also fortunate enough to be in several different metal bands, still play at church, and get the incredible opportunity to play guitar for many local community theaters. This kept his sight-reading in shape and gave him an appreciation for different styles of music (and paid pretty well, from a high schooler's perspective).
In 2001, Dennis came to Bexley, Ohio to study guitar at Capital University with Stan Smith. His studies emphasized jazz and classical guitar. Here his metal past merged with a deeper understanding of the instrument and music in general, and the basis for most of his teaching style was set in motion.
Dennis now plays guitar for Upper Arlington Lutheran Church every Sunday, for St. Christopher in Grandview, Ohio, with the youth group, and also plays for touring Broadway shows that stop in Columbus. Occasionally, he plays weddings and private parties, and he is starting a new cover band with some friends, called Dr. Awkward. He is blessed to have his understanding and supportive wife Kate, and is glad to be at JamPlay!
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I'm a fifty eight year old newbie who owns a guitar which has been sitting untouched in a corner for about seven years now. Last weekend I got inspired to pick it up and finally learn how to play after watching an amazing Spanish guitarist on TV. So, here I am. I'm starting at the beginning with Steve Eulberg and I couldn't be happier (except for the sore fingers :) Some day I'm going to play like Steve! I'm self employed with a hectic schedule. With Jamplay I can fit in a random session when I have time and I can go at my own pace, rewinding and replaying the videos until I get it. This is a very enjoyable diversion from my work yet I still feel like I'm accomplishing something worthwhile. Thanks a lot, Greg
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St Aloysius' College
Through my work, Masked, I explored two facets of human nature. The first, our uncanny ability to veil our inner selves so that our 'interface' with others reveals only what we want them to see; and second, the superficiality of our judgement of others. The masks represent how one's identity can be limited by an aesthetic perception. My sculptures are intended to be both comical and uncomfortably eerie. I hope an audience can see something strange yet familiar in the characters I have created – perhaps even something of themselves.
Art Chat: Joel Martorana (2:53)


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Finally, Kate and Jon are gone and we can get down to some serious reality TV and enjoy watching the trials and tribulations of some true American competitors, BBQ Pitmasters! TLC will feature this new series and we are proud to find out early that at least one of our featured Pitmasters, Mike Mills will be featured on the show. Here is a brief Synopsis:
Eight one-hour episodes of BBQ PITMASTERS have been ordered, and is scheduled to premiere December 3 on TLC. The series is produced by Original Media.
BBQ PITMASTERS transports viewers to this competitive cooking subculture, where an estimated 10 million people visit each year to get up-close and personal with the leading chefs of America's burgeoning barbecue circuit. Like an all-star poker tournament or a championship car race, it is at these events that reputations and respect are gained and lost. For pit masters, the honor rides on the virtues of one perfectly charred rib, and hundreds of thousands of dollars trade hands after a single, mouth-watering bite of brisket.
Finally they recognize that BBQ is not just a hobby, but a competitive sport. We cannot wait to see this and encourage you to check out BBQ Pro Shop where you can grill like a pro and use the rubs and sauces perfected and sold by the pitmasters featured on this show.


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DANVERS, Mass. — Philip Chism, the 14-year-old student charged in the slaying of Danvers, Massachusetts, high school teacher Colleen Ritzer, allegedly used a box cutter to kill her, two law enforcement sources close to the investigation said Thursday.
A source close to the investigation said Chism brought the box cutter to school with him. After killing Ritzer in a school bathroom, the source said, Chism allegedly rolled her body out of the building in a recycling bin and dumped her in the woods nearby.
The two law enforcement sources say Chism changed his clothes after killing the teacher.
Thursday, students and their parents can talk to grief counselors at Danvers High School, about a day after the beloved math teacher was found dead.
They and police investigators are asking why she was killed, and how a 14-year-old student could be accused of killing her. The answers may lie behind the police tape around this high school.
Those who knew both Chism and Ritzer were shocked.
"It's just surreal how quickly someone can go, and how much we take for granted everyday," said Danvers High School student Chris Weimert. Ritzer was "the nicest teacher you could ever have," he said. "I can't believe it."
A missing persons case
The ordeal started Tuesday as a missing persons case. Authorities first searched for Chism after he was reported missing. They alerted the public Tuesday evening that the teen had been last seen around 6:30 p.m. But around 11:20 p.m., they got a call that 24-year-old Ritzer had also disappeared.
She hadn't returned home and was not answering her phone, said Essex County District Attorney Jonathan Blodgett. Police in Topsfield, the town just north of Danvers, spotted Chism walking alongside a busy road there around 12:30 a.m.
At Danvers High, police found blood in a second-floor bathroom, the district attorney said. The teacher's body was soon discovered behind the school.
Detectives interviewed Chism, and whatever the teen told them about what went on at the school and in the surrounding woods, coupled with video surveillance, led to an arrest.
In court, it was alleged the teenager "did assault and beat" Ritzer, leading to her death.
"The juvenile was subsequently placed into custody and booked for murder," a criminal complaint says.
The 14-year-old was arraigned Wednesday afternoon in a Salem, Massachusetts, court for murder, then ordered held without bail. The Essex County District Attorney's office said in a statement it will seek a grand jury indictment for murder in the case. According to Massachusetts law, juveniles aged 14 or older are treated as adults by the court if they're charged with first- or second-degree murder.
A teacher who went the extra mile
Meanwhile, outside the courtroom, in the tight-knit community of Danvers, many were at a loss. Ritzer was beloved, her friends say. She posted inspirational messages on her Twitter account and went the extra mile to help students.
"She was talking on Saturday about this year was a good year. She was teaching freshmen for first time. She was happy," said Jen Berger, Ritzer's best friend. "I don't even know what the world is like without her. It's a scary thought."
Sympathy spread through the region, making its way into the baseball World Series. Bleachers full of fans who had assembled to watch the Boston Red Sox take on the St. Louis Cardinals observed a moment of silence in Ritzer's honor, before Game 1 began at Boston's Fenway Park.
Charlotte Dzerkacz in 2011 taught at the same middle school with Ritzer, during which time the two became close friends.
Ritzer seemed to always wear a wide smile and was approachable to students and colleagues alike.
"She was energetic, she was compassionate," Dzerkacz said. "You couldn't ask for anything more from a teacher or a friend."
A quiet and normal student
Chism is a quiet young man, those who know him said. He excelled at soccer and made a harmless impression.
"He … seemed quiet and reserved, but he just seemed normal," said Ariana Edwards, who was in Chism's English class.
Chism didn't drink or do drugs, and he came from a good family, one of his closest friends said. He described Chism as a good athlete who was shy at first but eventually warmed up to people, adding that he hadn't been acting strangely lately.
This friend and others got their first hint that something was awry when Chism didn't show up for soccer practice Tuesday. The team set out to look for him after seeing texts that he was missing.
As they and police were looking for him — and after Ritzer was killed — Chism went to the movies, a source with knowledge of the investigation said.
Chism was a newcomer to Danvers, a town of about 26,000 people. His family had bounced around as he lived in different cities in Tennessee and Florida, since he was in fourth grade, authorities in those states said.
Student suspect was in slain teacher's class
Blodgett, the prosecutor, declined Wednesday to offer specifics on the relationship between the suspect and the late teacher, though his office said Chism attended one of Ritzer's classes.
All seven schools in the North Shore town closed Wednesday, as investigators did their work and students and residents mourned the loss of Ritzer. Everything will reopen Thursday except the high school, but counselors and grief specialists will be available there for two hours during the day, said Superintendent Lisa Dana. Classes throughout the town are set to resume Friday, she said.
Ritzer graduated from Assumption College in 2011. She was working on getting a master's degree in school counseling at Salem State University, that school said.
"She believed children have much to offer and often do not realize how special they are as individuals," the university said. "In her application to Salem State she said she was dedicated to 'helping students in times of need.'"
Ritzer was known to take to Twitter to dole out homework assignments and wisdom to her students.
"No matter what happens in life, be good to people," she wrote in August. "Being good to people is a wonderful legacy to leave behind."
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At Toyota of Downtown LA, our large inventory and wide selection of models, along with competitive pricing, allow us to make you a great deal on the vehicle of your choice. Located in the heart of Downtown Los Angeles, we also serve Santa Monica, Hollywood and the surrounding areas. Se habla español.
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Rios avoids arbitration
TORONTO — After enduring an offseason that churned him up considerably in that pesky rumor mill, Alex Rios is officially on the books with the Blue Jays for the 2008 season.
On Tuesday, Toronto avoided arbitration with Rios, setttling on a one-year deal worth $4.835 million. For those without enough fingers to do the math with at home, that's a $2.3 million raise over what the right fielder took home in 2007, when he cashed in with the arbtration process for the first time.
I know, what about all that long-term contract talk? It's still on. This one-year deal was simply a stage in the process to forego an arbitration hearing. This way, Rios' contract for '08 is done and the Jays and Rios' reps can continue to discuss a multiyear deal during Spring Training.
If it doesn't get done before Opening Day, it'll probably have to wait until next offseason. Teams and players don't generally delve into negotiations during a season if they don't have to and Rios is controllable through 2010. So, if it ain't done now, it'll be a topic later on. Plenty of time.
Rios, who was seeking $5.6 million compared to Toronto's offer of $4.535 million, gets a signing bonus of $3.5 million and has a base salary of $1.335 million. He'll also make another $15K if he heads to the Bronx as an American League All-Star in July.
No feedback from GM J.P. Ricciardi for now, but us media types will surely get some comment from the man at tonight's State of the Franchise event in Toronto. I'm looking forward to some of the questions/complaints that fans might offer up tonight at this townhall meeting-like gathering. There's usually a few gems.
Stay tuned…
2 Comments
I'll quote friends of mine by saying this news is 'sick nicers'.
It's nice to have him back again, and not overpay for Rios since he's still controllable. Hopefully this gives a chance for Rios to prove what his long-term value really is. I know he's got good numbers and a great arm, but I'd like to see him get some better second half production before giving him a huge contract. The last two years he's has explosive first halves with severe declines in the second half, especially regarding his power numbers. He needs to perform in both halves of the season to make himself indispensible to this club.


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LOS ANGELES, Calif. – Ryan O'Neal says the prognosis is positive for his recovery from recently diagnosed Stage 2 prostate cancer.
The 70-year-old actor said in a statement released through his publicist on Monday that he was "shocked and stunned by the news" but feels fortunate the disease was caught early. He says he's expected to make a full recovery.
A memoir about his romance with actress Farrah Fawcett, "Both of Us: My Life With Farrah," will be published on May 1. Fawcett died in 2009 after a public battle with cancer.
O'Neal says he's grateful for the support of his friends and family and advocates regular exams since early detection is the best defence.
Higher stage numbers mean more extensive disease, with Stage 4 being the highest, according to the National Cancer Institute.


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Before Jack the Ripper terrorising Blackheath, there was Spring Heeled Jack. Part bogeyman used to scare small children, part mysterious criminal. He was popularised in Penny Dreadfuls – cheap serialised booklets, where he was depicted almost as a comic book anti-hero. Wonderful name, Penny Dreadfuls… I wonder what the modern online equivalent is?
Picture the scene in 1837 – poor Polly Adams, slightly the worse for wear staggering around Blackheath Fair, when a terrifying figure leaps out of the darkness, like the devil himself, with glowing eyes, and vomiting blue flame…
But was he real? Or just the product of an early mass hysteria? From the Black Cat Press:
He was also the first of his kind. While legends of strange phantoms have existed since the beginning of history, Spring Heeled Jack was the first to enter the official record as a real phenomenon, or at least as one whose witnesses could be found and would testify to the veracity of their experience. Part of this may have been due to the changing culture of the early nineteenth century, and the rise of mass printing technology, the first newspapers were largely responsible for the general publics awareness of the events, but there was also something unique about him. There were other similar changes at the time too, for instance the first identifiable witnesses to the infamous 'phantom horse and carriage' and the classic 'haunted mansion' also date to the early nineteenth century, perhaps for similar reasons, and even the aforementioned Bigfoot was first spotted in Canada in the mid 1830's according to some researchers, but none captured the public imagination as Jack did. He can in many ways be regarded as Britain's patron saint of weirdness.
Now, my much more appealing alternate theory. In the 1830s, the Marquis of Waterford developed a top secret version of what is now known as Power-Bocking. Rather than selling them to the public, and making his fortune, he decided to terrorise young maidens by leaping out at them instead. Just look at the video below, and imagine one of these heading straight for you as you wandered over the Heath in the dark… Eeek!
See Also
Really detailed page about Spring Heeled Jack
South East London Folklore Society
The legend of Spring Heeled Jack
Top image is from from Wilson's Almanac. Lower image from Wikipedia.


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| 13.1°C Belfast
PSNI chief constable Matt Baggott has said he stands by his apology following the collapse of the Hyde Park IRA bomb case.
The trial of John Downey, 62, for the 1982 blast, which killed four soldiers, was stopped because he mistakenly received a letter from the Government telling him he was not wanted by police.
In fact he was sought by the Metropolitan police but that was not communicated to him by officers in Northern Ireland.
A retired senior detective has dismissed any suggestion that an error was made by the Police Service of Northern Ireland (PSNI) and said the final letter included additions made by the Northern Ireland Office NIO.
Chief constable Matt Baggott said: "I am clear that the apology I gave in the aftermath of this judgment was appropriate.
"I stand by it."
Former detective chief superintendent Norman Baxter appeared before the Northern Ireland Affairs Committee on Wednesday to explain his role in the contentious on-the-runs administrative scheme, which saw letters sent to around 190 republicans informing them they were not sought by the authorities in the UK at that moment but did not rule out future prosecutions if new evidence emerged.
Details of the plan, which was agreed by Sinn Fein and the Government in the early 2000s as part of the peace process, emerged following the collapse of the case against Donegal man Mr Downey.
Names of individuals who feared they were wanted fugitives were passed to the Government, mostly through Sinn Fein, asking for an assessment of their status.
The Downey prosecution was stopped in February after a judge found he had been wrongly sent one of the so-called letters of comfort.
The message was sent by the NIO but was based on an assessment made by Mr Baxter that Mr Downey was not wanted by the police.
Separately, Mr Baxter alleged yesterday that Downing Street asked police in Northern Ireland to release two republican terror suspects hours after they had been arrested in connection with the attempted murder of a soldier.
Mr Baxter told the committee Sinn Fein president Gerry Adams had put pressure on Downing Street to ask for the release of Vincent McAnespie and Gerry McGeough, arrested for questioning about the attempted murder of part-time Ulster Defence Regiment soldier Sammy Brish in 1981.
McGeough was later convicted and sentenced for attempted murder.
The phone call from Government in 2007 was made at the request of Sinn Fein president Gerry Adams, Mr Baxter claimed.
Mr Adams has denied asking the Government to intervene with the PSNI.
Former chief constable and now Association of Chief Police Officers head Sir Hugh Orde has also said no such call was received from Downing Street, claiming he would have remembered it until his "dying day".
Mr Baggott said Mr Baxter should come forward with details behind his allegations and has written to the former officer asking him for his account.
He told the Policing Board in Belfast: "Mr Baxter will be asked to justify his comments."
He added: "It was a serious allegation made in the context of parliamentary privilege, we now want to assess exactly what the facts are.
"Once we have got that, if there is any further action necessary then we will take that forward."
Stormont justice minister David Ford separately defended his senior civil servant Nick Perry over claims the permanent secretary did not pass on information about the administrative scheme to him after he transferred from the NIO.
He said: "I completely reject the suggestion made during last week's (Justice) Committee meeting that my department is dysfunctional or that Mr Perry's actions have given me cause for embarrassment.
"I have a very good working relationship with Mr Perry and believe we carry out our respective roles as political and administrative heads of the department appropriately and effectively."
Lady Justice Hallett has been appointed by Prime Minister David Cameron to review the administrative scheme.
Belfast Telegraph


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2CV Racing Events
UK events are organised by the Classic 2CV Racing Club. In 1988 a handful of motor-sport loving 2CV drivers went to see the Belgian 24-hour race for 2CVs at Spa Francorchamps; they came back amazed and keen to start racing in the UK. Drivers often take corners two or three abreast and then slipstream the car in front before trying to overtake before the next bend. The racing is fiercely competitive; it is also relatively clean and good natured. You can have a day - or weekend - out with the family enjoying the summer weather, watching some of the closest and most exciting saloon car racing – and you can talk to the teams.
BBC TV News feature on the 2010 Snetterton 2CV 24 hour race >>
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- Hi-Lo Hem
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- Printed open 3/4 sleeve cardigan
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- Made in the USA
WE ONLY OFFER STORE CREDIT FOR RETURNS! We do not issue refunds back to your method of payment. We hope this will encourage you to take your time in selecting your purchases and feel free to reach out to us with any questions regarding sizing or styling. Store Credit never expires. We add new styles weekly and hope we can assist you in finding something new if your purchase doesn't work out! Store Credit will be sent to you in the form of an E-Giftcard via email when your Return is processed. It will be sent to the email address you have on file. SALE ITEMS, ACCESSORIES, BELTS, HANDBAGS, HATS, JEWELRY, INTIMATES + BODYSUITS ARE ALL FINAL SALE. Please visit our Returns Center on our website for further info.


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Police make arrest in long-barreled gun robbery
Williams to be arraigned Wednesday
Somerville police say they have arrested a man who brandished a long-barreled handgun during a convenience store robbery on Sunday.
Police said Jonathan Williams, 31, of Revere, was arrested on Wednesday in Woburn.
Police said Williams entered the Quick Stop Convenience store at 219 Highland Ave. on Sunday with the gun and demanded cash, "including the money in the box under the counter."
The man fled the scene fled with about $1,000 in cash, police said.
Police released surveillance pictures from the robbery and identified Williams through tips.
Williams will be arraigned in Somerville District Court on Wednesday.
Police said Williams is also suspected in as many as three other armed robberies in Medford on Jan. 24.
In surveillance images from the four robberies, the man is wearing a black knit cap with grey stripe.


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Springs Mennonite Church (Springs, Pennsylvania, USA)
Springs Mennonite Church (MC), located at Springs, 4 miles west of Salisbury, Pennsylvania, a member of the Allegheny Mennonite Conference, was founded in 1780. Until 1853 the congregation, known as the Society of Mennonites, met in homes for worship under Lancaster Mennonite Conference. The first minister was Jakob Seiler (later Saylor), an Amishman from Germany, who had been ordained bishop to serve in the Meyersdale area. In 1853, after a period of decline, Henry H. Blauch was ordained to the ministry, and in the half century of his service the church grew from 22 members to about 250 in the Casselman Valley district. In 1859 the Mennonite Union Church, known as Keim's, was built at St. Paul with the Lutheran and Reformed congregations. In 1874 a meeting was held here to consider the formation of the Southwestern Pennsylvania Conference, now the Allegheny Conference. In 1878 a meetinghouse was built at Springs, known first as the Folk Church. It was remodeled and enlarged in 1916 and 1925, and replaced by a new church in 1954. The product of the missionary outreach of this church are the Oak Grove, Casselman, Glade, Pinto, and Gortner Mennonite churches. Other mission points are Laughlin, turned over to the Brethren; Lageer, turned over to Glade; Bear Hill; Manadier; Fairview; Black Hawk, later called Meadow Mountain; Dry Run; Red Run; Bear Creek, and Meyersdale. The membership of the Springs congregation in 1957 was 291, with Roy Otto as bishop, and Walter Otto as pastor.
|Elmer E Bittinger
|Date Published
Cite This Article
MLA style
Bittinger, Elmer E. "Springs Mennonite Church (Springs, Pennsylvania, USA)." Global Anabaptist Mennonite Encyclopedia Online. 1959. Web. 28 Sep 2020. https://gameo.org/index.php?title=Springs_Mennonite_Church_(Springs,_Pennsylvania,_USA)&oldid=102830.
APA style
Bittinger, Elmer E. (1959). Springs Mennonite Church (Springs, Pennsylvania, USA). Global Anabaptist Mennonite Encyclopedia Online. Retrieved 28 September 2020, from https://gameo.org/index.php?title=Springs_Mennonite_Church_(Springs,_Pennsylvania,_USA)&oldid=102830.
Adapted by permission of Herald Press, Harrisonburg, Virginia, from Mennonite Encyclopedia, Vol. 4, p. 603. All rights reserved.
©1996-2020 by the Global Anabaptist Mennonite Encyclopedia Online. All rights reserved.


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|Sun Datacenter InfiniBand Switch 36 HTML Document Collection for Firmware Version 2.0
Network Management Connector and Pins
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Preparing to Install the Switch
Suggested Tools for Installation
Antistatic Precautions for Installation
Verify Shipping Carton Contents
Install the Switch in the Rack
Verifying the InfiniBand Fabric
Understanding Routing Through the Switch
Understanding Administrative Commands
Administering the I4 Switch Chip
Controlling the I4 Switch Chip
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Controlling the Subnet Manager
Administering the InfiniBand Fabric
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Understanding Oracle ILOM on the Switch
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Installing the Oracle ILOM Firmware
Acquire the Oracle ILOM Firmware Version 1.1.3
Install the Oracle ILOM Firmware Version 1.1.3
Administering Oracle ILOM (CLI)
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Disable the Locator LED (IPMI)
Understanding Oracle ILOM Commands
Understanding Service Procedures
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Remove the Switch From the Rack
Understanding Hardware Commands
Linux Shells for Hardware Commands
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Linux Shells for InfiniBand Commands
Understanding the SUN-DCS-IB-MIB MIB OIDs
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Understanding the ENTITY-MIB MIB OIDs
Enables or disables controlled handover.
setcontrolledhandover state|list
where state is either TRUE, or FALSE.
This hardware command enables or disables the controlled handover feature. The TRUE option enables and the FALSE option disables the action. The list option provides a listing of the Subnet Manager settings in respect to priority, controlled handover, and subnet prefix.
Note - You must stop or disable the Subnet Manager before using the setcontrolledhandover command. See disablesm Command.
The InfiniBand specification identifies two parameters used to negotiate a master Subnet Manager - the Subnet Manager priority and the port GUID. Should two or more Subnet Managers have the same highest configured priority, the Subnet Manager with the lowest GUID becomes the master Subnet Manager.
If an un-controlled or automatic handover state exists, when the primary-and-master Subnet Manager fails, the secondary-and-standby Subnet Manager takes on the role of the new master Subnet Manager. During this time, traffic is temporarily suspended as the secondary-and-master Subnet Manager discovers and sets up the InfiniBand fabric.
If the primary Subnet Manager is able to recover from the failure, reboot, and reinitialize itself, it identifies itself as the intended master Subnet Manager. Again, traffic is temporarily suspended as the primary-and-master Subnet Manager re-discovers and sets up the InfiniBand fabric. The secondary Subnet Manager returns to the standby status.
If controlled handover is enabled, then some priority values become reserved. The Subnet Managers dynamically adjust their respective priorities to avoid the dual handover situation. A secondary Subnet Manager that became a master Subnet Manager due to a handover raises its priority to a reserved value and retains its master Subnet Manager status. Regardless if the primary Subnet Manager comes back online, the second handover does not occur.
The user-configured priorities of the Subnet Managers are retained, only during a handover are reserved priorities used.
The result of this scheme is that the user-configured priority is respected during system boot, but dual handover does not occur because the primary Subnet Manager loses its master Subnet Manager status (secondary Subnet Manager priority changes to reserve).
Note - Manually starting Subnet Managers individually is equivalent to the system boot sequence.
The setcontrolledhandover command is available from the /SYS/Fabric_Mgmt Linux shell target of the Oracle ILOM CLI interface.
This example shows how to enable a controlled handover of Subnet Managers with the setcontrolledhandover command.
# setcontrolledhandover TRUE Current SM settings: smpriority 5 controlled_handover TRUE subnet_prefix 0xabbababe M_Key None #