text:
Abstract: Deficiencies of antioxidant nutrients have been implicated in the etiology of lung and other cancers. However, most intervention trials with antioxidant nutrients have not shown beneficial effects, and some have indicated that beta-carotene may be deleterious. This randomized, doubleblind, placebo-controlled study evaluated the effects of five short-term ( 4-wk) antioxidant nutrient supplement regimens [ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), betacarotene (60 mg), selenium (80 mug as sodium selenite), ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg)] on plasma antioxidants and mononuclear leukocyte DNA damage in male smokers (n = 9) and nonsmokers (n = 12). Plasma concentrations of ascorbic acid and tocopherol were significantly increased by supplementation, but there was no significant change in plasma beta-carotene or blood glutathione peroxidase activity after supplementation with betacarotene or selenium. DNA damage in mononuclear leukocytes, as assessed by comet assay, was not affected by any supplementation regimen. DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocytes, was not influenced by ascorbic acid, alpha-tocopherol, or selenium supplementation in smokers or nonsmokers, but beta-carotene supplementation resulted in significant differences between smokers and nonsmokers in the level of oxidative DNA damage, with decreases in smokers and increases in smokers. This is a further indication of the differential effects of supplemental beta-carotene in smokers and nonsmokers.
Reactive oxygen species (ROS), generated in vivo, have the potential to cause damage to DNA and other biomolecules and thus have the potential to initiate cancers and other chronic diseases ( 1). There is an array of nutrient-dependent, nonenzymatic and enzymatic mechanisms that can modulate ROS-mediated damage. Antioxidant nutrients include ascorbic acid, tocopherols, and carotenoids, which act as scavengers of ROS, and trace elements, such as copper or selenium, which, as part of antioxidant enzymes, have roles in the elimination of ROS ( 2).
A number of long-term ( 4-12yr) intervention studies have investigated the effects of dietary antioxidant supplementation on subsequent cancer onset and mortality. Promising results were obtained in a study in China, in which daily supplementation with a combination of [beta-carotene (15 mg), vitamin E (30 mg), and selenium (50 mug) resulted in reductions in total mortality and mortality from cancer ( 3). However, this population was not well nourished, and the effects may not be relevant to populations with an adequate intake of these nutrients. In trials in developed countries with smokers ( 4-6) and with a cohort of physicians ( 7), daily supplementation with beta-carotene ( 20-30mg), alone or in combination with vitamin A ( 25,000 IU) or tocopherol (50 mg), did not show beneficial effects on cancer outcomes. Indeed, there were indications that intervention with beta-carotene may increase cancer incidence, cancer mortality, and total mortality ( 4-6).
An observational epidemiological study and shorter-term supplementation studies have used markers of DNA damage, rather than cancer incidence, as the end point to investigate the significance of dietary and plasma antioxidants in smokers and nonsmokers at a molecular level. In a cross-sectional, observational study of Japanese adult male smokers and nonsmokers, lymphocyte DNA adduct levels were not significantly higher in smokers, and differences in lymphocyte DNA adduct levels were not related to differences in plasma beta-carotene or alpha-tocopherol in smokers or nonsmokers ( 8). Supplementation with ascorbic acid (250 mg) or tocopheryl acetate (100 mg) daily for two months, alone or in combination, had no effect on the urinary excretion of the DNA adduct 8-oxo-7,8-dihydro-2'-deoxyguanosine in male smokers ( 9). Supplementation with ascorbic acid (100 mg), alpha-tocopherol (280 mg), and beta-carotene (25 mg) daily for 20 weeks decreased endogenous DNA damage, as assessed by oxidation of pyrimidines, and also increased lymphocyte resistance to exogenous ex vivo DNA damage in smokers and nonsmokers ( 10). However, it was notable that, after only five weeks on this regimen, DNA damage, as assessed by oxidized pyrimidines, was significantly higher in the smokers than in the nonsmokers ( 10). Thus short-term supplementation studies with smokers and nonsmokers may provide insights into the way in which antioxidant nutrients impact on molecular damage that is potentially carcinogenic.
Cancer incidence is particularly high in Northern Ireland, a region where smoking is prevalent in a relatively high proportion of the population (11). The aim of the present study was to compare the effects of antioxidant nutrient supplements on mononuclear leukocyte DNA damage in smokers and nonsmokers, as assessed by two methods: the comet assay, which, as applied here, measures DNA single-strand breaks and alkali-labile sites, and the analysis of 8hydroxydeoxyguanosine, which is an oxidation product formed by the hydroxylation of guanine.
Materials and Methods
Subjects and Intervention Protocol
Twenty-one men, aged 26-59 years, were recruited from the academic and technical staff of the University of Ulster. All subjects were apparently healthy, were on no long-term medication, and were not taking other dietary supplements. Nine were smokers and 12 were nonsmokers. Smokers reported smoking at least one cigarette per day for at least eight years, and the nonsmokers reported never smoking or not smoking for at least one year. The trial lasted 40 weeks and used a placebo-controlled, randomized, double-blind, multiple-crossover design. Each treatment period lasted four weeks, with a four-week washout period between treatments, when the subjects received placebo. Entry to the study was staggered over four weeks. At entry, subjects were randomly allocated to placebo or one of the supplements for the first four-week period. The order of the supplements was randomized. It was found post hoc that 5 of the 9 smokers and 6 of the 12 nonsmokers had received placebo in the first four-week period. The supplements were ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), beta-carotene (60 mg), selenium (80 mug as sodium selenite), and ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg). Placebo and supplements were in tablet form (Larkhall Natural Health, London, UK). Blood (40 ml) was taken from subjects at entry and at the end of each four-week period into lithium heparin tubes. Plasma was separated from blood (8 ml) by centrifugation (400 g, 20 min, 4 degrees C) immediately after collection. Plasma aliquots for analysis of alpha-tocopherol, beta-carotene, and ascorbic acid were frozen in liquid nitrogen and stored at -80 degrees C. Plasma aliquots for ascorbic acid analysis were diluted 1:1 with 10% aqueous metaphosphoric acid before freezing. Aliquots of whole blood were also stored at -80 degrees C before analysis of hemoglobin and glutathione peroxidase activity. All antioxidant analyses were carried out in single batches at the end of the intervention, and storage times ranged from 1 to 10 months. Anthropometric measurements (height, weight, waist, hip, and skinfold thicknesses) were made at the start and end of the study period. Body mass index (kg/m2) and percent body fat were calculated using appropriate equations ( 12, 13). Habitual intake of macronutrients and micronutrients was assessed at the end of the study by using a modified ( 14) seven-day diet history ( 15) and a computerized food composition database (Foodbase, Royal Society of Chemistry, Cambridge, UK).
Plasma and Blood Antioxidant Analysis
Plasma alpha-tocopherol and beta-carotene were measured by high-performance liquid chromatography (HPLC) ( 16) on 100-mul aliquots, with tocopheryl acetate as internal standard. Pooled plasma samples with each set acted as internal quality controls, and external standards were used for calibration. The coefficients of variation (CVs) for alpha-tocopherol and beta-carotene analyses were 4.6% and 6.3%, respectively. Ascorbic acid in plasma was measured by a modified HPLC method ( 17) on 50-mul aliquots, with pooled plasma and standard ascorbic acid (40 muM) as controls; the CV was 7.2%. Selenium-dependent glutathione peroxidase, a functional marker for selenium status, was measured on whole blood by means of an enzymatic-spectrophotometric method (Ransel, Randox Laboratories, Crumlin, UK), and activity was calculated per gram of hemoglobin. The CV for the glutathione peroxidase assay was 9.4%. Hemoglobin was measured using 40 ml of whole blood, which were added to 20 ml of Isoton (Coulter Electronics, Luton, UK). After mixing by inversion, six drops of Zapoglobin (Coulter Electronics) were added. Hemoglobin was then measured as cyanmethemoglobin in a hemoglobinometer (Coulter Electronics).
Analysis of 8-Hydroxydeoxyguanosine in Mononuclear Leukocyte DNA
Immediately, after collection, whole blood (30 ml) was mixed with 30 ml of Dutch modified RPMI 1640 at 20 degrees C; this mixture was layered in four 15-ml aliquots onto 15-ml aliquots of lymphocyte separation medium (Nycomed, Oslo, Norway) and centrifuged (700 g, 30 min, 20 degrees C). The mononuclear leukocytes were isolated from the phase interfaces with a glass pipette, combined, washed twice [50 ml of Dutch modified RPMI and 20 ml of tris(hydroxymethyl)aminomethane (Tris)-buffered saline, pH 7.4], resuspended in 1 ml of freezing buffer (10 mM Tris, 400 mM NaCl, 2 mM disodium EDTA, pH 8.0), frozen in liquid nitrogen, and stored at -80 degrees C. At the end of the study, thawed mononuclear leukocytes were lysed in proteinase K buffer (Sigma Chemical, Poole, UK), and the cellular proteins were salted out by dehydration and precipitation. After ribonuclease treatment, DNA was precipitated in ethanol, redissolved in nitrogen-saturated buffer, and stored at -20 degrees C before hydrolysis of DNA with deoxyribonuclease I, alkaline phosphatase, and phosphodiesterases I and II. Deoxyguanosine and 8-hydroxydeoxyguanosine were determined by HPLC ( 18) with use of coulometric detection. The 8-hydroxydeoxyguanosine was calculated as moles per 105 mol of deoxyguanosine. The CV for this analysis in our laboratory is normally <5%.
Assessment of DNA Damage in Mononuclear Leukocytes (Comet Assay)
Whole fresh blood (30 mul) was added to 1 ml of RPMI 1640 (without phenol red; containing 10% fetal calf serum), mixed, left to stand for 30 minutes on ice, then underlaid with 100 mul of Histopaque 1077 (Sigma Chemical) and centrifuged (200 g, 5 min, 4 degrees C). Mononuclear leukocytes were isolated from the interface and resuspended in 1 ml of phosphate-buffered saline on ice. These cells were irradiated with X-rays at 4 degrees C to doses of 1 or 5 Gy at a dose rate of 2.65 Gy/min (300 kV Siemens Stabilipan). Unirradiated (control) mononuclear leukocytes were also prepared. Mononuclear leukocyte DNA damage was assessed using the comet assay ( 19) with minor modifications ( 20, 21). DNA damage was assessed as tail moment [(percentage of DNA in the tail x distance between head and tail centers) + 100]. Fifty cells were assessed for each treatment. The CV for this assay is about 10% ( 22).
Data were analyzed by repeated-measures analysis of variance and independent t-tests, with a Bonferroni correction used for multiple comparisons.
There was no significant difference between the smokers and nonsmokers for age, height, weight, or body mass index (Table 1) or in percent body fat or waist and hip measurements (data not presented). Although generally low, alcohol intakes were significantly and substantially higher in the smokers (Table 1). There was no significant difference between smokers and nonsmokers in the intakes of other macronutrients, fiber, or selected antioxidant micronutrients (Table 1). Although plasma concentrations of ascorbic acid, alpha-tocopherol, and beta-carotene and blood glutathione peroxidase activity were higher before supplementation in the nonsmokers than in the smokers, none of these differences was significant (Table 2). Analysis of the data for each supplementation regimen at the end of the respective subsequent placebo periods showed no significant differences, indicating that carryover effects were minimal. Analysis of the effects of supplementation on the respective plasma antioxidants showed that supplementation with ascorbic acid and alpha-tocopherol, singly or in combination, led to significant increases in the concentrations of these antioxidants in the plasma of smokers and nonsmokers (Table 2). Although supplementation with beta-carotene increased plasma beta-carotene by 26% in smokers and 9% in nonsmokers, these effects were not significant. Supplementation with selenium did not significantly affect selenium-dependent glutathione peroxidase activity (Table 2). Mononuclear leukocyte DNA damage, as assessed by the comet assay on unirradiated cells, was not influenced by any supplementation regimen in smokers or nonsmokers (Table 3). This DNA damage was increased by exogenous radiation doses (1 and 5 Gy), but the level of damage was not influenced in smokers or nonsmokers by any supplementation regimen (data not presented). Supplementation with selenium or with ascorbic acid and alpha-tocopherol, alone or in combination, had no effects on the level of oxidative DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocyte DNA, in smokers or nonsmokers (Table 4). However, there was a significant difference between smokers and nonsmokers in response to supplementation with beta-carotene, with 8-hydroxydeoxyguanosine concentrations decreased in nonsmokers but increased in smokers (Table 4).
Although there were significant increases in plasma ascorbic acid and alpha-tocopherol concentrations in response to supplementation with these nutrients (Table 2), these were not accompanied by any significant effects on the level of mononuclear leukocyte DNA damage, as assessed by comet assay or by 8-hydroxydeoxyguanosine (Table 3). These findings concur with recent results which showed that supplementation of male smokers with ascorbic acid (250 mg) or tocopheryl acetate (100 mg) daily for two months, either alone or in combination, had no effect on DNA damage, as assessed by urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine ( 9). Together, these results indicate that supplementation with ascorbic acid or alpha-tocopherol, alone or in combination, is ineffective at reducing oxidative DNA damage or DNA damage as assessed by strand breaks. The lack of a relationship between plasma alpha-tocopherol and DNA damage also supports a recent cross-sectional study in Japan with male smokers and nonsmokers, which found not only that lymphocyte DNA adduct levels were not significantly higher in smokers, but also that individual differences in lymphocyte DNA adduct levels were not related to differences in plasma alpha-tocopherol in smokers or nonsmokers ( 8). Furthermore, in that study, no relationship was found between lymphocyte DNA adduct levels and plasma beta-carotene levels in either group, and it was concluded that neither alpha-tocopherol nor beta-carotene is protective against smoking-induced DNA damage ( 8). However, the study was observational, and subjects were not supplemented with either micronutrient.
A protective role for supplemental beta-carotene against DNA damage was suggested in a study where smokers and nonsmokers were supplemented with ascorbic acid (100 mg), alpha-tocopherol (280 mg), and beta-carotene (25 mg) daily for 20 weeks ( 10). In that study, supplementation had no significant effects on DNA damage, as assessed by strand breaks, in smokers or nonsmokers. When DNA damage was assessed as oxidation of pyrimidines, supplemented smokers and nonsmokers showed substantial and highly significant reductions in DNA damage after 20 weeks. However, at five weeks, although the supplement had no significant effect on oxidized pyrimidines in either group, there was evidence of a differential effect, and the level of oxidized pyrimidines was significantly and substantially higher in the smokers ( 10). Differential responses in smokers and nonsmokers to short-term beta-carotene supplementation is also indicated in the present study, where four weeks of supplementation with beta-carotene (60 mg) showed that oxidative DNA damage, as assessed by mononuclear leukocyte 8-hydroxydeoxyguanosine, increased in smokers but decreased in nonsmokers. However, beta-carotene supplementation had no effect on the level of DNA damage, as assessed by strand breaks (comet assay). Taken as a whole, it appears that supplemental alphatocopherol, ascorbate, and selenium do not affect the levels of DNA damage but that supplemental beta-carotene can impact oxidative DNA damage but not DNA damage as assessed by strand breaks; furthermore there are differential responses in smokers and nonsmokers, which are dependent on the duration of supplementation.
The reasons for the different responses of smokers and nonsmokers to beta-carotene supplementation are not clear. One possible explanation is an interaction with alcohol consumption. Alcohol consumption is difficult to assess accurately, but despite the relatively modest intakes reported here, alcohol was the only nutrient to show a significant difference, with smokers having a substantially higher intake than nonsmokers. Interactions between alcohol and beta-carotene are indicated in other studies. For example, a cross-sectional study of smokers and nonsmokers showed no difference in alcohol intake between the two groups, but alcohol consumption and smoking were significantly negatively correlated with plasma beta-carotene ( 8). Furthermore, in two major antioxidant intervention trials with smokers, increases in cancer incidence were associated with higher alcohol intakes ( 23, 24). The beta-carotene supplements used here and in other studies are many times greater than normal intakes. Little is known of the biological effects of these high doses of beta-carotene, which may have prooxidant effects, or of the effects of beta-carotene metabolites, which may increase when high levels of beta-carotene are given ( 25). Toxic tissue levels of beta-carotene or its metabolites may result from the coingestion of beta-carotene and alcohol ( 26), and there may be other potentially damaging interactions between betacarotene, alcohol, and vitamin A ( 25). The mechanisms whereby long-term beta-carotene supplementation may increase cancer incidence in smokers remain unclear. However, it appears that short-term ( 4-5wk) supplementation with beta-carotene can result in increased levels of DNA damage in smokers compared with nonsmokers.
Acknowledgments and Notes
This work was supported by The Cancer Research Campaign. Address reprint requests to Dr. R. W. Welch, Northern Ireland Centre for Diet and Health, School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK.
Submitted 6 October 1998; accepted in final form 1 April 1999.
Table 1. Age, Anthropometric Data, and Estimated Daily Intake of Energy, Macronutrients, Fiber, and Selected Antioxidant Nutrients for Smokers and Nonsmokers[a,b]
Smokers Nonsmokers
(n = 9) (n = 12)
Age, yr 40.6 +/- 2.7 43.7 +/- 2.0
Height, m 1.70 +/- 0.02 1.77 +/- 0.01
Weight, kg 71.4 +/- 2.7 80.6 +/- to 2.7
BMI, kg/m2 24.4 +/- 0.9 25.6 +/- 0.7
Total energy, kJ 9,099 +/- 540 8,805 +/- 642
Protein, g 72.1 +/- 4.4 81.3 +/- 5.5
Fat, g 80.4 +/- 6.4 76.2 +/- 6.2
Carbohydrate, g 267 +/- 16 274 +/- 21
Alcohol, g 22.5 +/- 8.6 7.9 +/- 4.3[*]
Dietary fiber,[c] g 13.4 +/- 1.6 18.8 +/- 2.7
Ascorbic acid, mg 77 +/- 14 74 +/- 8
Vitamin E, mg 71 +/- 8 100 +/- 12
beta-Carotene, mug 2,440 +/- 260 2,453 +/- 246
Retinol, mug 713 +/- 307 688 +/- 286
Retinol equivalents, mug 1,121 +/- 334 1,119 +/- 298
Selenium, mug 37 +/- 4 51 +/- 7
a: Values are means +/- SE; n, number of subjects. BMI, body mass index.
b: Statistical significance is as follows: *, significantly different from smokers, p < 0.05.
c: Nonstarch polysaccharides, Englyst method.
Table 2. Plasma Antioxidants Before and After Supplementation With Relevant Micronutrients in Smokers and Nonsmokers[a,b]
Legend for Chart:
A - Supplement
B - Plasma
C - Smokers, (n = 9), Before
D - Smokers, (n = 9), After
E - Nonsmokers, (n = 12), Before
F - Nonsmokers, (n = 12), After
Ascorbic acid (350 mg) 27.7 +/- 2.7 54.5 +/- 10.4[*]
Ascorbic acid, mumol/l 32.3 +/- 2.0 45.1 +/- 5.6[*]
alpha-Tocopherol (250mg) 23.6 +/- 1.1 30.8 +/- 3.1[*]
alpha-Tocopherol, mumol/l 25.5 +/- 0.8 28.6 +/- 2.0[*]
beta-Carotene (60 mg) 0.27 +/- 0.05 0.34 +/- 0.05
beta-Carotene, mumol/1 0.46 +/- 0.08 0.50 +/- 0.09
Selenium (80 mug) 40.2 +/- 1.4 41.3 +/- 4.9
GPx,[c] U/g Hb 41.9 +/- 1.2 38.9 +/- 3.4
Ascorbic acid (350 mg) 27.7 +/- 2.7 48.7 +/- 7.9[*]
Ascorbic acid, mumol/l 32.3 +/- 2.0 43.3 +/- 5.8[*]
+ alpha-tocopherol (250 mg) 23.6 +/- 1.1 31.6 +/- 2.5[*]
alpha-Tocopherol, mumol/l 25.5 +/- 0.8 32.9 +/- 2.5[*]
a: Values are means +/- SE at end of placebo period preceding supplementation with relevant micronutrient (Before) and at end of supplementation period with relevant micronutrient (After); n, number of subjects.
b: Statistical significance is as follows: *, significant difference between placebo and supplementation, p < 0.05.
c: Selenium-dependent glutathione peroxidase (GPx) activity per gram of hemoglobin (Hb).
Table 3. Mononuclear Leukocyte DNA Damage of Smokers and Nonsmokers Before and After Micronutrient Supplementation, as Assessed by Comet Assay on Unirradiated Samples[a,b]
Legend for Chart:
A - Supplement
B - Smokers (n = 9), Before
C - Smokers (n = 9), After
D - Nonsmokers (n = 12), Before
E - Nonsmokers (n = 12), After
Ascorbic acid 1.50 +/- 0.45 3.61 +/- 2.50
3.19 +/- 1.30 3.84 +/- 1.86
alpha-Tocopherol 4.49 +/- 2.22 3.40 +/- 1.41
1.95 +/- 0.63 1.76 +/- 0.62
beta-Carotene 2.19 +/- 0.45 3.62 +/- 0.90
1.65 +/- 0.42 1.57 +/- 0.35
Selenium 2.02 +/- 0.48 2.59 +/- 1.07
2.41 +/- 0.77 2.77 +/- 1.22
Ascorbic acid + 1.50 +/- 0.49 3.40 +/- 1.41
alpha-tocopherol 2.25 +/- 0.68 3.82 +/- 1.15
a: Tail moments.
b: Values are means +/- SE at end of placebo period preceding supplementation with relevant micronutrient (Before) and at end of supplementation period with relevant micronutrient (After); n, number of subjects.
Table 4. 8-Hydroxydeoxyguanosine in Mononuclear Leukocyte DNA of Smokers and Nonsmokers Before and After Micronutrient Supplementation[a-c]
Legend for Chart:
A - Smokers (n = 9), Before
B - Smokers (n = 9), After
C - Nonsmokers (n = 12), Before
D - Nonsmokers (n = 12), After
Ascorbic acid 3.70 +/- 0.41 5.91 +/- 2.39
6.86 +/- 2.42 3.09 +/- 0.80
alpha-Tocopherol 6.31 +/- 2.77 3.93 +/- 1.97
4.83 +/- 0.89 4.30 +/- 0.99
nu-Carotene 2.58 +/- 0.59 3.52 +/- 0.81[*]
5.25 +/- 1.38 2.24 +/- 0.36[*]
Selenium 6.52 +/- 2.25 12.98 +/- 4.97
5.35 +/- 1.70 6.28 +/- 2.04
Ascorbic acid + 5.79 +/- 2.15 3.99 +/- 0.95
alpha-tocopherol 11.26 +/- 6.14 4.63 +/- 1.19
a: 8-Hydroxydeoxyguanosine as moles per 102 mol deoxyguanosine.
b: Values are means +/- SE; n, number of subjects. c: Statistical significance is as follows: *, responses significantly different in smokers and nonsmokers (p < 0.05). An outlier in smokers after supplementation (46.50) was excluded before data were tested using repeated-measures analysis of variance followed by an independent t-test with a Bonferroni correction.
1. Halliwell, B: Antioxidants in human health and disease. Annu Rev Nutr 16, 33-50, 1996.
2. Strain, JJ, Hannigan, BM, and McKenna, PG: The pathophysiology of oxidant damage. J Biomed Sci 2, 19-24, 1991.
3. Blot, WJ, Li, J-Y, Taylor, PR, Guo, W, Dawsey, S, et al.: Nutrition intervention trials in Linxian, China: supplementation with specific vitamin/mineral combinations, cancer incidence, and disease-specific mortality. JNCI 85, 1483-1491, 1993.
4. Heinonen, OP, Huttunen, JK, Albanes, D, Haapakowski, J, Palmgren, J, et al.: The effect of vitamin E and beta-carotene on the incidence of lung cancer and other cancers in male smokers. N Engl J Med 330, 1029-1035, 1994.
5. Albanes, D, Heinonen, OP, Huttunen, JK, Taylor, PR, Virtano, J, et al.: Effects of alpha-q and beta-carotene supplements on cancer incidence in the alpha-Tocopherol beta-Carotene Cancer Prevention Study. Am J Clin Nutr 62, 1427S-1430S, 1995.
6. Omenn, GS, Goodman, GE, Thornqvist, MD, Balmes, J, Cullen, MR, et al.: Effects of a combination of beta-carotene and vitamin A on lung cancer and cardiovascular disease. N Engl J Med 334, 1150-1155, 1996.
7. Hennekens, CH, Buring, JE, Manson, JE, Stampfer, M, Rosner, B, et al.: Lack of effect of long-term supplementation with beta-carotene on the incidence of malignant neoplasms and cardiovascular disease. N Engl J Med 334, 1145-1149, 1996.
8. Wang, Y, Ichiba, M, Oishi, H, Iyadomi, M, Shono, N, et al.: Relationship between plasma concentrations of beta-carotene and alpha-tocopherol and life-style factors and levels of DNA adducts in lymphocytes. Nutr Cancer 27, 69-73, 1997.
9. Prieme, H, Loft, S, Nyyssonen, K, Salonen, JT, and Poulsen, HE: No effect of supplementation with vitamin E, ascorbic acid or coenzyme Q10 on oxidative DNA damage estimated by 8-oxo-7,8-dihydro-2'-deoxyguanosine excretion in smokers. Am J Clin Nutr 65, 503-507, 1997.
10. Duthie, SJ, Ma, A, Ross, MA, and Collins, AR: Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res 56, 1291-1295, 1996. 11. Barker, ME, McClean, SI, McKenna, PG, Reid, NG, Strain, JJ, et al.: Diet, Lifestyle and Health in Northern Ireland. Coleraine, UK: University of Ulster, 1988.
12. Schofield, WN: Predicting basal metabolic rate: new standards and reviews of previous work. Clin Nutr 39C Suppl 1, 5-41, 1985.
13. Dumin, JVGA, and Womersley, J: Body fat assessed from total body density and its estimation from skinfold thickness measurements on 481 men and women aged from 16-72 years. Br J Nutr 32, 77-97, 1974.
14. Cunningham, K, and Lee, D: The Irish National Nutrition Survey. Dublin: Irish Nutrition Dietetic Institute, 1990.
15. van Staveren, WA, deBoer, JD, and Burema, J: Validity and reproducibility of a dietary history method estimating the usual food intake during one month. Am J Clin Nutr 42, 554-559, 1985.
16. Thumham, DI, Smith, E, and Flora, PS: Concurrent liquid-chromatographic assay of retinol, alpha-tocopherol, beta-carotene, alpha-carotene, lycopene and beta-cryptoxanthin in plasma with tocopherol acetate as internal standard. Clin Chem 34, 377-381, 1988.
17. Heiliger, F: Measurement of plasma ascorbate. Curr Separations 2, 368-372, 1980.
18. Shigenaga, MK, Park, JW, Cundy, KC, Guineno, CJ, and Ames, BN: In vivo oxidative DNA damage: measurement of 8-hydroxy 2'-deoxyguanosine in DNA and urine by high-performance liquid chromatography with electrochemical detection. Methods Enzymo1186, 521-530, 1990.
19. Singh, NP, McCoy, MT, Tice, RR, and Schneider, EL: A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175, 184-191, 1988.
20. McKelvey-Martin, V J, Green, MHL, Schmezer, P, Pool-Zobel, BL, DeMeo, MP, et al.: The single cell gel electrophoresis assay (comet assay): a European review. Mutat Res 288, 47-63, 1993.
21. Sweetman, SF, Strain, JJ, and McKelvey-Martin, VJ: Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cells. Nutr Cancer 27, 122-130, 1997.
22. Collins, A, Dusinska, M, Franklin, M, Somorovska, M, Petrovska, H, et al.: Comet assay in human biomonitoring studies: reliability, validation and applications. Environ Mol Mutagen 30, 139-146, 1997.
23. Albanes, D, Heinonen, OP, Taylor, PR, Virtamo, J, Edwards, BK, et al.: alpha-Tocopherol and beta-carotene supplements and lung cancer in the alpha-Tocopherol, beta-Carotene Study: effects of baseline characteristics and study compliance. JNCI 88, 1560-1570, 1996.
24. Omenn, GS, Goodman, GE, Thomqvist, MD, Balmes, J, Cullen, MR, et al.: Risk factors for lung cancer and for intervention effects in CARET, The [3-Carotene and Retinol Efficacy Trial. JNCI 88, 15501559, 1996.
25. Erdman, JW, Russell, RM, Mayer, J, Rock, CL, Barua, AB, et al.: beta-Carotene and the carotenoids: beyond intervention trials. Nutr Rev 54, 185-188, 1996.
26. Ahmed, S, Leo, MA, and Lieber, CS: Interactions between alcohol and beta-carotene in patients with alcoholic liver disease. Am J Clin Nutr 60, 430-436, 1994.
By R.W. Welch; E. Turley; S.F. Sweetman; G. Kennedy; A.R. Collins; A. Dunne; M.B.E. Livingstone; P.G. McKenna; V.J. McKelvey-Martin and J.J. Strain


text:
4 Using Failover Manager : 4.1 Managing a Failover Manager Cluster
Once configured, a Failover Manager cluster requires no regular maintenance. The following sections provide information about performing the management tasks that may occasionally be required by a Failover Manager Cluster.By default, some of the commands listed below must be invoked by efm or by an OS superuser; an administrator can selectively permit users to invoke these commands by adding the user to the efm group. The commands are:To start the Failover Manager cluster on RHEL 6.x or CentOS 6.x, assume superuser privileges, and invoke the command:To start the Failover Manager cluster on RHEL 7.x or CentOS 7.x, assume superuser privileges, and invoke the command:If the cluster properties file for the node specifies that is.witness is true, the node will start as a Witness node.If the node is not a dedicated Witness node, Failover Manager will connect to the local database and invoke the pg_is_in_recovery() function. If the server responds false, the agent assumes the node is a Master node, and assigns a virtual IP address to the node (if applicable). If the server responds true, the Failover Manager agent assumes that the node is a Standby server. If the server does not respond, the agent will start in an idle state.After joining the cluster, the Failover Manager agent checks the supplied database credentials to ensure that it can connect to all of the databases within the cluster. If the agent cannot connect, the agent will shut down.If a new master or standby node joins a cluster, all of the existing nodes will also confirm that they can connect to the database on the new node.You can add a node to a Failover Manager cluster at any time. When you add a node to a cluster, you must modify the cluster to allow the new node, and then tell the new node how to find the cluster. The following steps detail adding a node to a cluster:
1. Unless auto.allow.hosts is set to true, use the efm allow-node command, to add the IP address of the new node to the Failover Manager allowed node host list. When invoking the command, specify the cluster name and the IP address of the new node:efm allow-node cluster_name ip_addressFor more information about using the efm allow-node command or controlling a Failover Manager service, see Section 5.Install a Failover Manager agent and configure the cluster properties file on the new node. For more information about modifying the properties file, see Section 3.5.1.
3. To start the Failover Manager cluster on RHEL 7.x or CentOS 7.x, assume superuser privileges, and invoke the command:When the new node joins the cluster, Failover Manager will send a notification to the administrator email provided in the user.email property, and/or will invoke the specified notification script.Please Note: To be a useful Standby for the current node, the node must be a standby in the PostgreSQL Streaming Replication scenario.If your Failover Manager cluster includes more than one Standby server, you can use the efm set-priority command to influence the promotion priority of a Standby node. Invoke the command on any existing member of the Failover Manager cluster, and specify a priority value after the IP address of the member.For example, the following command instructs Failover Manager that the acctg cluster member that is monitoring 10.0.1.9 is the primary Standby (1):You can set the priority of a standby to 0 to make the standby non-promotable. Setting the priority of a standby to a value greater than 0 overrides a property value of promotable=false.For example, if the properties file on node 10.0.1.10 includes a setting of promotable=false and you use efm set-priority to set the promotion priority of 10.0.1.10 to be the standby used in the event of a failover, the value designated by the efm set-priority command will override the value in the property file:In the event of a failover, Failover Manager will first retrieve information from Postgres streaming replication to confirm which Standby node has the most recent data, and promote the node with the least chance of data loss. If two Standby nodes contain equally up-to-date data, the node with a higher user-specified priority value will be promoted to Master. To check the priority value of your Standby nodes, use the command:efm cluster-status cluster_namePlease note: The promotion priority may change if a node becomes isolated from the cluster, and later re-joins the cluster.You can invoke efm promote on any node of a Failover Manager cluster to start a manual promotion of a Standby database to Master database.Manual promotion should only be performed during a maintenance window for your database cluster. If you do not have an up-to-date Standby database available, you will be prompted before continuing. To start a manual promotion, assume the identity of efm or the OS superuser, and invoke the command:efm promote cluster_name [-switchover]
[-sourcenode <address>] [-quiet] [-noscripts]cluster_name is the name of the Failover Manager cluster.Include the –switchover option to reconfigure the original Master as a Standby. If you include the –switchover keyword, the cluster must include a master node and at least one standby, and the nodes must be in sync.Include the –sourcenode keyword to specify the node from which the recovery.conf file will be copied to the master.Include the -quiet keyword to suppress notifications during switchover.Include the -noscripts keyword to prevent instruct Failover Manager to not invoke fencing and post-promotion scripts.
• A recovery.conf file is copied from an existing standby to the master node.
• The address of the new master node is added to the recovery.conf file.
• If the recovery.conf file contains an application name, and the application.name property is set for this node, the application name will be replaced with the property value.During a manual promotion, the Master agent releases the virtual IP address before creating a recovery.conf file in the directory specified by the db.recovery.conf.dir property. The Master agent remains running, and assumes a status of Idle.The Standby agent confirms that the virtual IP address is no longer in use before pinging a well-known address to ensure that the agent is not isolated from the network. The Standby agent runs the fencing script and promotes the Standby database to Master. The Standby agent then assigns the virtual IP address to the Standby node, and runs the post-promotion script (if applicable).Please note that this command instructs the service to ignore the value specified in the auto.failover parameter in the cluster properties file.efm set-priority cluster_name ip_address priorityefm promote cluster_name ‑switchoverWhen you stop an agent, Failover Manager will remove the node's address from the cluster members list on all of the running nodes of the cluster, but will not remove the address from the Failover Manager Allowed node host list.To stop the Failover Manager agent on RHEL 6.x or CentOS 6.x, assume superuser privileges, and invoke the command:To stop the Failover Manager agent on RHEL 7.x or CentOS 7.x, assume superuser privileges, and invoke the command:Until you invoke the efm disallow-node command (removing the node's address of the node from the Allowed node host list), you can use the service efm-3.5 start command to restart the node at a later date without first running the efm allow-node command again.To stop a Failover Manager cluster, connect to any node of a Failover Manager cluster, assume the identity of efm or the OS superuser, and invoke the command:efm stop-cluster cluster_nameThe command will cause all Failover Manager agents to exit. Terminating the Failover Manager agents completely disables all failover functionality.Please Note: when you invoke the efm stop-cluster command, all authorized node information is lost from the Allowed node host list.The efm disallow-node command removes the IP address of a node from the Failover Manager Allowed node host list. Assume the identity of efm or the OS superuser on any existing node (that is currently part of the running cluster), and invoke the efm disallow-node command, specifying the cluster name and the IP address of the node:efm disallow-node cluster_name ip_addressThe efm disallow-node command will not stop a running agent; the service will continue to run on the node until you stop the agent (for information about controlling the agent, see Section 5). If the agent or cluster is subsequently stopped, the node will not be allowed to rejoin the cluster, and will be removed from the failover priority list (and will be ineligible for promotion).After invoking the efm disallow-node command, you must use the efm allow-node command to add the node to the cluster again. For more information about using the efm utility, see Section 5.3.
4 Using Failover Manager : 4.1 Managing a Failover Manager Cluster

subset: commoncrawl


text:
Lensel Optics Pvt Ltd
Mr. VIjay Shelar & Mr. Milind Doshi
Address :
66/2, Block D2, MIDC Chinchwad, Midc, Pune, Maharashtra - 411019, India
Phone No :
+91-20-27463647, +91-20-27474340, 66111722
Fax :
Mobile :
+91-9404962731, +91-9422650076
Leveraging on our rich industry experience and knowledge, we bring Contract Manufacturing Services in Maharashtra, India. We are backed by a team, containing optical and mechanical engineers, to entertain the best range of engineering services. Clients can approach us for acquiring glass optics, plastic optics, coatings, assembly, etc. In addition, we make use of high-grade material and sophisticated technology for manufacturing them. We work on ethics and are concerned about intellectual property rights, so we sign an agreement with our patrons, which we pursue in letter and spirit.
Some Of The Products Recently Developed At Lensel By Our Customers
Galilean Telescopes With Led Light. :
Glass Lens Assemblies :
Otoscope : We have developed complete imaging systems consisting of aspheric plastic lenses for use in the medical field. A US based medical devices company recently developed an Otoscope with us using 3 aspheric lenses. Due to the biological limitations of the human ear, the front lenses of this system were only 2.20 mm in diameter with complex, multi-coefficient higher order aspheric surfaces. The product produced sharp images of the inner ear and has been approved by our customer. We manufacture the lenses, coat them, assemble them and do the complete product testing for the customer.
Periscope For Nuclear Reactor :
Reverse Engineering Of Optical Layouts : Our skilled team of optical engineers is able to reverse engineer any optical system. Our thorough study includes de-bonding of doublets, study of all optical parameters, determination of optical glass used and transmission curve of the coating used. Using these techniques that have been honed over the years, we have achieved a very high success rate in our reverse engineering jobs. Some of our more challenging reverse engineered complex multi-lens systems include a Night Vision Binocular for an Indian defense company, a range of medical imaging devices for a Brazilian customer, fiber optic coupler etc.


text:
Ebola Strains West Africa's Health Care Infrastructure, Ebola Expert Says
Scientific American: How Ebola Strains West Africa's Infrastructure
"Ebola has pummeled health care systems in West Africa and laid bare health infrastructure issues that may not have otherwise been so readily apparent. It has also prompted aid organizations and governments to do some soul-searching about what should be done to prepare for the future. … [The magazine interviews] Ebola expert Daniel Bausch, a professor in the Department of Tropical Medicine at Tulane University, [who] has been consulting with groups including the World Health Organization to ramp up its Ebola response…" (Maron, 11/7).


text:
The Royal Masquerade is the a fantasy filled night at The Castle!
In addition to your favorite DJs we're bringing in a touch of old world style with amazing classical musicians!
We will be showcasing wonderful harpist, cellist and violinist in the Dungeon!
We will also have Forrest Outman leading the Midnight Waltz!
The Beauty & Beast Ball is a formal event where you MUST be in formalwear (a suit and mask at the least) or costume or pay an additional $10 party pooper fine!
18 to enter and 21 to drink!


text:
Guest House for Young Widows: Among the Women of ISIS (Hardcover)
Not in stock. Order here for delivery or to pickup later.
A gripping account of thirteen women who joined, endured, and, in some cases, escaped life in the Islamic State—based on years of immersive reporting by a Pulitzer Prize finalist.
FINALIST FOR THE BAILLIE GIFFORD PRIZE • NAMED ONE OF THE TEN BEST BOOKS OF THE YEAR BY PUBLISHERS WEEKLY AND ONE OF THE BEST BOOKS OF THE YEAR BY The New York Times Book Review • NPR • Toronto Star • The Guardian
Among the many books trying to understand the terrifying rise of ISIS, none has given voice to the women in the organization; but women were essential to the establishment of Abu Bakr al-Baghdadi's caliphate.
Responding to promises of female empowerment and social justice, and calls to aid the plight of fellow Muslims in Syria, thousands of women emigrated from the United States and Europe, Russia and Central Asia, from across North Africa and the rest of the Middle East to join the Islamic State. These were the educated daughters of diplomats, trainee doctors, teenagers with straight-A averages, as well as working-class drifters and desolate housewives, and they joined forces to set up makeshift clinics and schools for the Islamic homeland they'd envisioned. Guest House for Young Widows charts the different ways women were recruited, inspired, or compelled to join the militants. Emma from Hamburg, Sharmeena and three high school friends from London, and Nour, a religious dropout from Tunis: All found rebellion or community in political Islam and fell prey to sophisticated propaganda that promised them a cosmopolitan adventure and a chance to forge an ideal Islamic community in which they could live devoutly without fear of stigma or repression.
It wasn't long before the militants exposed themselves as little more than violent criminals,more obsessed with power than the tenets of Islam, and the women of ISIS were stripped of any agency, perpetually widowed and remarried, and ultimately trapped in a brutal, lawless society. The fall of the caliphate only brought new challenges to women no state wanted to reclaim.
Azadeh Moaveni's exquisite sensitivity and rigorous reporting make these forgotten women indelible and illuminate the turbulent politics that set them on their paths.
About the Author
Azadeh Moaveni is a journalist, writer, and academic who has been covering the Middle East for nearly two decades. She started reporting in Cairo in 1999 while on a Fulbright fellowship to the American University in Cairo. For the next several years she reported from throughout the region as Middle East correspondent for Time magazine, based in Tehran, and covered Lebanon, Syria, Egypt and Iraq. She is the author of Lipstick Jihad, Honeymoon in Tehran, and co-author, with Shirin Ebadi, of Iran Awakening. In November 2015, she published a front-page piece in The New York Times on ISIS women defectors that was a Pulitzer Prize finalist as part of the Times's ISIS coverage. Her writing appears in The Guardian, The New York Times, and The London Review of Books. She teaches journalism at NYU in London, is a fellow at the New America foundation, and is now senior gender analyst at the International Crisis Group.
Praise For…
"A master class in illustrating the big picture through small stories . . . Azadeh Moaveni has written a powerful, indispensable book on a challenging subject: the inner lives and motivations of women who joined or supported the Islamic State militant group. . . . An illuminating, much-needed corrective to stock narratives, not only about the group that deliberately and deftly terrified officials and publics across the world, but also about the larger 'war on terror' and the often ineffective, even counterproductive policies of Western and Middle Eastern governments."—Anne Barnard, The New York Times Book Review
"In this searing investigation, Moaveni explores the phenomenon of Muslim women—many of them educated, successful, and outwardly Westernized—choosing to travel to Syria in support of jihad. . . . In concise, visceral vignettes, Moaveni immerses her readers in a milieu saturated with the romantic appeal of violence. The result is a journalistic tour de force that lays bare the inner lives, motivations, and aspirations of her subjects."—Publishers Weekly (starred review)
"Azadeh Moaveni has achieved a feat of reporting to provide a rare glimpse into the private lives of these ordinary people caught up in extraordinary events. Brave, visceral, moving; essential reading for anyone seeking to understand so much of the violence in our troubled world."—Ben Rawlence, author of City of Thorns


text:
Unlicenced Construction In Marmaris Demolished In Ceremony
A giant shopping center with an area of 23 thousand 300 square meters and a multi-storey car park were demolished based on the ground of illegal construction.
While "Illegal housing" is again the subject of debate after the earthquake in Van, Marmaris Council started to demolish a giant shopping center with an area of 23 thousand 300 square meters and a multi-storey car park with a ceremony.
In his speech durin the ceremony, CHP Marmaris Mayor Ali Acar said "This structure, which we see as the embodiment of ugliness was stabbed like a dagger at the heart of Marmaris in 1996 with a 'fait accompli'. The mentality transforming the most important area of our city into a stack of concrete without asking the public, has turned this place into a commercial mass of concrete where should be of common use areas. I'm happy to keep our promise while we were aspiring to work."
Acar said they aim to turn the public lands on either side of the existing building of which they initiated the demolition into parks and green areas.
Demolishing the building will last for approximately a month and then the construction work for the "Town square" will start. Green areas, artificial waterfalls, water show pools and a clock tower will take place at the square, and it is scheduled to enter service in mid-May.


text:
Does your Office Design attract top candidates (or repel them)?
So you're in hiring mode. You're hardly alone – and that's why it's so difficult to attract and successfully hire the best candidates. In today's increasingly competitive market, you may feel like you've already done everything in your power to tip the scales in your favor. But have you? If you've managed to keep a top candidate interested throughout the interviewing process – but lose them at offer time, take a look around your office. Then ask yourself this: when is the last time you took a peek at the digs your competitors are offering? Sure, you've probably replace a few torn carpet tiles, maybe brought in a few plants… But when is the last time you made a substantial investment in the look, feel and composition of your work spaces? Maybe it's time you did.
There's been a lot of buzz over the past few years about employer branding and marketing. I'm assuming that you've already done the hard work of defining your employer brand, creating a marketing strategy, and selling your brand throughout the recruiting process. But all too often, recruiters and hiring managers forget one critical component of the employer branding and marketing process:
Work Space Packaging.
That's right. I said packaging. Think about it. If you want to truly attract and retain the best talent available, you need to think like a marketer – at every level. Why would your target audience want to join your organization? Why would they want to keep coming back to work every day? When they're in your environment, how productive, creative and simply happy does it make them feel?
A recent article at Inc.com explores the need for more "vitality" in our work spaces. I found their focus on "biophilic" ("nature loving") design trends particularly compelling, especially for northern climate employers trying to attract top talent from out-of-state.
While you've been investing in highly collaborative, super productive teams, it's possible that you've neglected to support those teams with a positive physical work environment. Yet, as anyone from Minnesota can tell you, the climate (and access to sunlight) that your office space creates has a dramatic effect on an employee's willingness to show up every day. Check out these office design trends that every employer should consider.
1. Bring Nature Indoors with Biophilic Design
If the idea of water features, plant walls and massive skylights strike you as extravagant fads, they're probably not. Considering the fact that most of us spend upwards of 90% of our time indoors, it's understandable that we feel better, think better and work better when we're better connected to the sights, sounds and fresher air of nature. A number of recent scientific studies have proven that to be the case. Am I talking about adding plastic potted ficus trees near people's cubicles? No. True Biophilic design even extends beyond living potted plants. If you're serious about bringing this philosophy into your space, you'll think about it as more of an architectural philosophy that incorporates natural light and living plants as well as the forms, textures, even sounds (such as moving water) of nature itself. It's a growing trend that is becoming especially popular in those areas where it's simply tougher for employees to access nature every day. Not ready to take the deep, green plunge? Start with desk top terrariums and see how it affects your mood and those of everyone around you.
2. "Unconventional" Work Areas
Not everybody does their best work while chained to the desk eight hours a day. This design approach, led largely by the growing population of millennial workers, accommodates a desire for collaborative spaces, wall-free zones, even atypical seating such as sofas, floor pillows and other design elements that encourage settling in, stretching out and hunkering down for serious brainstorming. If it all sounds too far out for you, do a few online searches of the term "unconventional office space" and you'll be amazed by how this approach – practiced by highly successful employers around the word – can work.
3. "Homestyle Comfort" Design
Exactly what its name implies, this approach brings the comforts of a happy home into the work space. Sure, you may have a company fridge but do you have it stocked with fresh juices and sparkling water? What about a fireplace or a game area? At Skywater, we've devoted the center of our office area to game zone, complete with a basketball hoop. It's one of the smartest investments we've ever made. Bringing homestyle comfort in starts with asking yourself what people need in order to feel at home. If you're located within easy walking distance to coffee shops and restaurants, great. If you're not, can you attract food trucks or provide healthy food options within the confines of your building? Providing these things shows your employees that you care about their needs and you listen to their wants. Plus, it has the added advantage of encouraging people to stick around, hang out and collaborate with each other more.
4. Dynamic Spaces
A growing trend that's taken hold recently, dynamic spaces allow for multiple uses and frequent movement or change. When I hear dynamic space described as "that space for the creatives," I feel the need to remind people of this: we all need to be – and feel – creative in our jobs. You can make your workspace more dynamic by choosing furniture that's light weight, on casters, or easily moveable in other ways. This not only allows your employees to switch up their work environment to keep things fresh, it allows you to maximize the functions of limited square footage.
5. Go ahead. Add a little color.
Even if you're sticking with gray cubicle walls, you don't need to stick with a pale gray environment. Splashes of bold color can reinvigorate an otherwise tired looking space. But be sure to choose colors that complement each other and the environment you're trying to create. Think of bold colors like music. Turn up the volume a little bit. But crank it too loud and you'll overwhelm everybody's senses.


text:
Baby Prams and Strollers
Our baby prams and strollers have been hand picked by our owners and are practical, reliable and well designed for a wide range of parents life styles. You can buy them online or in our Brisbane baby store. Statistics show that parents buy 5 baby prams / strollers over the life of a child on average.
This means that baby stores are not doing a good job of understanding each customers individual needs and guiding them to the right pram or stroller for their specific needs.
We have learned that every parent's needs are different when it comes to buying baby prams and strollers. Everyone has a different life style, different cars (boot sizes) etc. This means as a parent, you can't just take a recommendation from another friend and go with that because their needs will be very different. On top of that, too many baby stores offer way too many models and brands that are similar and confuse first time parents.
We have chosen models and brands of prams and strollers that offer unique benefits and address different parents needs without confusing them. See our range below and remember that you can always contact our professional team to get advice and guidance. We don't want you to become a statistic and end up buying 5 prams / strollers.
We only source our prams and strollers from brands here in Australia. They all meet the Australian Standards and they all have genuine Australian Manufacturer's warranty. When buying prams online, you have to be careful where they were sourced from. You don't need to worry when you buy from us in store or online.
If you are local to our store – come in and meet our professional team who will spend time to assist you to choose the best baby pram for your needs out of our range of baby prams.
Joolz Day3$1,497
SAVE Up to $372 Off RRP!
The Joolz Day3 will enable you as a parent to stroll smarter, move easier and live better. Make it happen every day with the Joolz Day3. The clever blend of style and functionality makes the Joolz Day3 the perfect pram to handle all of life's tasks, little and big. The see-through ventilation in the hood and bassinet allows a flow of fresh air, while the head cushion on the mattress and adjustable footrest provide extra support for your child's head and feet.
- Up to 21% Off! Ridiculous Price! Limited Stock
Joie Nitro LX Compact Stroller
SAVE Up to $42.95 Off RRP!
The Joie Nitro LX Compact Stroller is a handy travel mate designed and engineered to keep up with your child from newborn on. Ready to roll with everything needed to keep you and your mini strolling with ease. Stylish travelling This fun, functional and fashionable on-the-go solution is sleek and modern Light and luxe Lightweight, umbrella chassis along with durable plush fabrics is handy for users and cosy for riders Built to last Designed to go and go and go—with 100% premium, highest quality materials One Comfortable Ride Loaded with comfort-inspired upgrades like an adjustable legrest, a soft bar and harness covers SPECIFICATIONS Model: S1036BFCRB600 Product Weight: 7.66kg Open Size: l 82.4 x w 48.7 x h 108cm Folded Size: l 31 x w 27.5 x h 108cm Product Usage: birth to 20kg Testing Certification: EN1888-2012
Uppababy Rumble Seat
SAVE Up to $52.95 Off RRP!
The Uppababy Rumble Seat can be used forward facing, rear facing and recline – it's the multitasking stroller seat you have only dreamed of. While one child is enjoying a snack, the other can be enjoying a catnap. Your family has multiple needs, and your VISTA stroller has the flexibility and comfort to take them on the road in style – all while strolling like a single.
UPPAbaby bassinet$327 – $347
SAVE Up to $72.95 Off RRP!
The UPPAbaby Bassinet provides a perfectly padded and snug bassinet attachment for your UPPAbaby Cruz or Vista pram. Transform your Cruz or Vista into a pram ideal for your newborn with our bassinet accessory. Whether for naps on the go or overnight sleeping, the Alta or Vista bassinet allows your baby to lie completely flat. Our Cruz / Vista Bassinet complies with new ASTM standards requiring; (1) the bassinet to be at least 191mm in depth and (2) canopy hinges be engaged by pressing a button to both raise and lower the canopy.
3 Sprouts Stroller Organiser
SAVE Up to $4.95 Off RRP!
Make your pram life easier with the 3 Sprouts Stroller Organiser! Keep all of your essentials organised and in reach when you are out for a walk with your child. The 3 Sprouts Stroller Organiser is completely able to be wiped clean. In addition, it has two insulated drink holders! This makes it perfect for a sippy cup or bottle.
OutLook Shade a Babe
SAVE Up to $25 Off RRP!
Outlook Shade a Babe Pram Shade zips open for accessibility while still providing side shade and glare protection for delicate eyes. Now you can access your child without needing to take the shade off totally!!! Shade a Babe - Combine fun, fashion & style with UV and glare protection for your child Shade a Babe's flexible 3-layered design provides all-round protection from the sun with no need to continuously adjust the shade. Shade a Babe Helps block harmful UV Rays UPF50+ sun visor ? the highest UPF (Ultraviolet Protection Factor) fabric rating available. Shade a Babe Shields delicate eyes UPF10 glare shield. A dual mesh layer underneath the visor assists safeguard from sunlight and glare when visor pulled back. Shade a Babe Protects from insects and wind UPF3 lower mesh safeguards from insects and wind and screens Ultra violet rays getting into lower part of the pushchair, while still providing excellent vision and air flow. Main area unzips for simple use of kid. Shade a Babe has a Universal fit Fits 3 & 4 wheel prams and strollers.


text:
Now available in iTunes Podcasts and on www.satanicstorytime.com Satanic Story Time's 12th episode, "Phantoms" Part 1, is now available for download via iTunes Podcasts and at http://www.satanicstorytime.com. Mathew Stover (M.R. Stover) joins us as our special guest for an interview and allows me to narrate his most recent short story entitled, "Phantoms" Part 1. I uncover the mailbag to delve into some of the listeners' recent thoughts. We listen to my narration of Mathew Stover's "Phantoms" Part 1. We get to hear my recent interview with Mr. Stover. In the interview, Mathew talks about his story, "Phantoms". He explains when he first became interested in writing and his fascination with the macabre. We briefly touch on the mythos of phantoms. And Mathew tells us where the listeners can obtain updates on his projects and find material he has to offer.
To obtain updates and announcements regarding Mathew Stover's latest stories, upcoming books and other publications, please follow him on Twitter @Twomrstover. Additionally, please visit www.lulu.com and search for "Nightmare Engine" Issue 6; this is Michael K. Silva's magazine. And in Issue 6 of The Nightmare Engine, you will find another one of M.R. Stover's short stories, "Therianthrop". Mathew briefly touches on the content within the pages of "Therianthrop" during our interview, but if you want to read it for yourself, this is where to find it. Also, check out more issues from Michael K. Silva's magazine, The Nightmare Engine.


text:
A short epilogue from "After the End". She had such an intense level of faith in him. He would not fail either of them.
Annie Thinks: Pono is, as the summary says, an epilogue for a full-length story by templeton21. I was very wary of this going in. I thought it would be hard to follow and confusing, considering I hadn't read the story it was for. I expected to be disappointed, but I was very far from it.
Pono is about Jason and Jules, a couple who sticks together after the end of the world. They roam from town to town, trying to find warmth and safety, which is becoming increasingly harder to find and even more important since Jules is four months pregnant with Jason's child.
Although it isn't completely clear what caused the apocalypse, certain clues point to zombies, or, as they call them, Walkers. I'm a total zombie enthusiast, so the fact that they were called Walkers also made me wary because, in the amazing TV show "The Walking Dead," zombies are also referred to as Walkers. However, it was evident that Pono isn't fanfiction. Jason and Jules are original characters who have their own original stories, though they evoke the same fear and heartbreak as Rick Grimes' story. Jason and Jules have to find their own strength or at least fake it, especially since all they have is each other. The ending is very bittersweet--and, I'll be honest, I teared up a little bit. Their love for each other and for their unborn child is strong and fierce, even stronger and fiercer than their fear of losing one another. Pono is a truly beautiful, heartbreaking story that, in my opinion, captures the emotional aspect of being some of the last people on Earth perfectly.


text:
Union Public Service Commission (UPSC), New Delhi issued Notification for National Defence Academy (NDA) and Naval Academy (NA) Examination (I) 2013 for admission to the Army, Navy and Air Force wing of the NDA for 131st Course and Naval Academy:
NDA and NA Examination 2013
Total Post: 355 (Army-195, Navy-39, Air Force - 66, Navy Academy- 55)
Age: Born between 2nd January, 1995 and 1st July 1997.
Age: Born between 2nd January, 1995 and 1st July 1997.
Qualification: 12th Class pass of the 10+2 pattern of School Education or equivalent examination conducted by a State Education Board or a University.
Application Fee: Rs.100/- (No fee for SC/ST/ Sons of JCOs, NCOs, ORs).
How to Apply: Eligible candidates should apply ONLINE from UPSC official website http://upsconline.nic.in/mainmenu2.php on or before 21st January 2013
Last date: 21st January 2013
For Job Notification CLICK HERE


text:
Liberal Democrat candidate for North East Herts Amy Finch on why you should vote for her in the General Election
PUBLISHED: 16:40 19 November 2019 | UPDATED: 15:19 11 December 2019
Amy Finch, the Liberal Democrat candidate for North East Herts, has explained in her own words why you should vote for her in the 2019 General Election.
"I have lived in North Herts for many years, I grew up here and attended local schools in Letchworth.
"I joined the Liberal Democrats two years ago as I strongly believe that we should be building a fairer and more equal society. I believe that our country deserves better and that we need a new approach to politics, one that not only listens to our concerns but openly engages and unites our communities.
"We collectively face many challenges, yet the paralysis that Brexit brings is stopping us from addressing these pressing issues. I voted remain in the European referendum, and I am convinced more now than ever that any form of Brexit will damage our jobs, our economy and our public services. By stopping Brexit we can allow our economy to thrive and in turn invest in our public services.
You may also want to watch:
"I feel very fortunate to have grown up in Hertfordshire, surrounded by nature. The climate crisis is having an irreversible impact on our planet.
"Addressing the climate emergency must be one of our highest priorities and the Liberal Democrats have an ambitious environmental plan that includes planting 60million trees which would help reverse the decline of biodiversity, as well as fast tracking deployment of clean energy to tackle carbon emissions.
"I am deeply concerned by the rise of foodbanks and fuel poverty in our area. An emergency 10-year programme to insulate low-income homes, thus cutting bills, would go a long way to tackling the fuel poverty that many are currently experiencing here in Hertfordshire.
"A Liberal Democrat government would also look to make work pay and tackle in-work poverty, by implementing £50 billion of extra funding towards initiatives as the Work Allowance.
"In recent years' I worked for a non-profit that explored how technology could help our young people with mental resilience. Here I worked alongside Child & Adolescent Mental Health Services teams (CAMHS) which afforded me direct experience of the overwhelming pressures that these NHS services face. Therefore, I am particularly proud of the Liberal Democrats continuous work and commitment to prioritising mental health services.
"By investing £11 billion into mental health services we aim to match waiting time standards to those in physical health."
If you value what this story gives you, please consider supporting the Royston Crow. Click the link in the orange box above for details.


text:
Privacy Policy
Information gathered from visitors
As is typical for other websites, details such as the visitor's IP address, browser type, referring page and time of visit are stored in log files on the web server.
Cookies may be used to remember visitor preferences when interacting with the website.
Information gathered from registered users
Where registration is required, the visitor's email, chosen username, password, city, home state, home country, and ReaperList details will be stored on the server.
Use of gathered information
The information is used by the website to display personalized content and advertising. Use of this information will make the website more useful to visitors.
E-mail addresses will not be sold, rented or leased to 3rd parties.
E-mail may be sent to registered users to inform them of comments or messages added to their list by other users. The ReaperList newsletter will also be sent to registered users.
Visitor Options
If you choose not to continue receiving emails from ReaperList.com, you may unsubscribe by following the instructions included in all ReaperList.com e-mails.
Using your browser settings may allow you to block cookies. This will prevent you, however, from creating a new account or logging into an existing account.
Cookies are small files that your web browser stores on your computer. They contain your preferences and are used to enhance your experience with the site. They may also be used to track your return visits to the website.
Cookies on your computer may also be used by 3rd party advertising companies for tracking purposes.
Google Ads
We use third-party advertising companies such as Google to serve ads when you visit our website. These companies may use information (not including your name, address, email address, or telephone number) about your visits to this and other websites in order to provide advertisements about goods and services of interest to you. If you would like more information about this practice and to know your choices about not having this information used by these companies, click here.


text:
Internet, meet Bond.
Bond, internet. Bond was my Sushi Instructor last week at Sea to You Sushi and Asian Foods in Brookline. His laugh is infectious, and he finds his students to be particularly hilarious when it's obvious that they have no idea what they're doing. He's been teaching the art of sushi for twelve years now, but it was pretty clear to my group of rag-tag wannabe sushi chefs that the man really loves his job. My first bite of a basic California Roll wasn't until my third year of college, but my obsession with the little bite-sized (and sometimes not-so-bite-sized) rolls has become extravagant. I may have even told someone recently that I could eat sushi every single day and never tire of it. In 2008, I made an attempt to make my own sushi at home with a "sushi kit," but the rolls came out the size of Frisbees no matter how hard I tried! They were still delicious of course, but I felt like a rookie, and I knew I needed some guidance. Fast forward to June 2010, and the leadership I was looking for finally appeared: in the shape of Bond. Sushi Instructor Bond. First, Bond laid down the rules and told us all about sushi-grade fish, how to take care of ingredients properly, and how to not get food poisoning (in a nutshell). Then we all went around the room to introduce ourselves to the group, an activity I usually loathe with every fiber of my being, until Bond told us we could say what our favorite sushi roll was. I enthusiastically shouted "UNAGI!!" like a cheerleader. The promotion of eel consumption is a cause that I take very seriously. Why? Because it's delicious, and more people should give it a shot. Trust. After the scare tactics, it was time to get our hands dirty. An array of sushi ingredients were on display on a nearby bar: Crab meat, cucumber, avocado, sweet potato (wait, what?), and of course sticky rice and seaweed paper. Bond then gathered us all around to demonstrate the proper way to make a sushi roll, which I will now share with you. First, place the seaweed paper (also called "nori") on your bamboo rolling mat. Wrapping the rolling mat in plastic wrap beforehand makes clean-up a lot easier. Then, take a large snowball-sized amount of sticky rice and plop it down on the nori sheet. Spread it all over so you get a nice thick layer of rice.
Once the sticky rice is actually sticking, flip it over so that the rice is on the outside and the nori is on the inside. Then you can start piling on the crab meat and cucumbers and a Happy Meal or whatever else you want in your sushi. Next, it's time to roll! I'm not going to lie, I was very nervous about this part since my past experience with this part of the deal didn't go so well (FRISBEES!). But Bond told us something about rolling sushi that changed my life. "Cat paws." Yep, that's the secret to getting tight, perfectly round sushi rolls. Make cat paws with your hands and SCRUNCH that roll until your knuckles hurt. Observe the final product.
Perfection. Lastly, Bond showed us how to use the knife to cut our perfectly rolled sushi and sent us off into the great unknown to make our own individual sushi creations. The good news: my first attempt wasn't terrible!
The bad news (for me): My friend Abby's sushi came out better.
Some people are just blessed with the innate power to sushi. Sigh. Regardless, I'm so glad I took this class with Instructor Bond. I have a greater understanding of WHY sushi is made the way it is made, and my prior love/obsession has only grown stronger. Plus I made enough to sushi to feed myself for the entire weekend. :) Thank you to Bond and Sea to You Sushi for a wonderful night! — Alison


text:
Mizuno Wave Inspire 13
shopping_cart Buy Now
$119.99 $99.99
The Mizuno Wave Inspire 13 helps correct your running stride for a smooth, more natural run from your first step to the last.; Engineered mesh upper for a breathable, flexible fit that moves with you.; U4ic midsole for respnsive energy return with every step.; Double Fan Wave adds support, while sculpted lateral forefoot creates a more stable platform.; U4icX heel wedge adds soft cushioning where you need it most.; Durable rubber outsole for traction on any surface.; Wt. 9.3 oz.


text:
Using advice and guidance supports the NHS long term plan to use digital technology to redesign clinical pathways and reduce unnecessary hospital referrals.
Toolkit feedback and further questions
This toolkit is designed to support all aspects of e-RS advice and guidance. However if you have further questions or would like to provide feedback please contact email@example.com.
Last edited: 20 April 2020 3:23 pm


text:
Hi, my name's Sammy and I'm the Editor of Simply Prizes magazine. Every week I'll be bringing you an update or two about any interesting competitions I find or any comping news. Plus I'll bring you any competitions or offers I find.
Now if you're pretty good at putting pen to paper then put the kettle on and take a look at this! Dragonfly tea has launched their 2016 short story competition. With a theme of 'discovery' this creative comp is open to all 'as yet unknown' writers. There is a maximum word limit of 3,000 but no minimum and the winner and runner ups will be decided by a panel of judges.
In partnership with the Henley Literary Festival a group of short-listed entrants will be invited to a special prize giving ceremony at the festival on Sunday 2 October where the winners will be announced. The prizes are as follows; 1st prize £1500, 2nd prize £750 and 3rd prize £250.
The closing date is 29th August and entry can be made online here (link 1), scroll to the bottom of the page.
On top of that they also have a children's comp running too. With a theme of 'adventure', a minimum word limit of 500 and three age categories (4-7, 8-11, 12-15) this could be a good way of keeping the kiddies amused for a day or two over half term! Each winner will receive a £50 voucher and £100 worth of book vouchers for their school. Closing date is also 29th August.
The full terms and conditions can be found here and I would recommend you give them a read before entering.
Good luck all!
Sammy x


text:
Posted by Willy Franzen on June 19, 2013. Jobs updated daily.
|Used Vehicle Manager
Bethesda, MD
|Enterprise Sales Manager
Herndon, VA
|Dynamics CRM Practice Lead - Remote (East Cost Preferred)
Herndon, VA
|Store Manager
Leesburg, VA
|Customer Service - FULL TIME
Silver Spring, MD
|Business Develoment Manager
Fairfax, VA
|Marketing Manager
Fairfax, VA
|Digital Marketing Manager
Arlington, VA
|Sales Representative
Rockville, MD
|Sales Representative - Hiring Now
Rockville, MD
Sales is all about relationships. Whether you're trying to convince someone to make a one time purchase or enter a ten-year contract, you need to build a certain level of trust and empathy (the level is much higher in the latter case). Sometimes it only takes one conversation, but it often takes many more. A sales person has to be organized to ensure that his or her communications are continuously moving towards a sale. The tools for doing that used to be a rolodex and a notebook, but that has obviously changed. What hasn't changed is the need for mobility in a customer relationship management system, and that's exactly what Base CRM offers. They are a Chicago, IL based company that "is paving the way by building the next generation of CRM software." They realized that most competing software is a burden (not a useful tool) for salespeople, so they decided to build something that people would actually want to use.
Base CRM has a lofty goal–they want to "make you and your team 10x more productive." I don't know how close they are to accomplishing that, but they already can claim big names like White Castle, Century 21, Nationwide, and Vienna Beef as customers. You can learn about what kind of results Base CRM has accomplished for its customers by checking out their Success Stories. Base CRM's software is built on the freemium model, so you can try it for free, but have to pay up if you really want to get the most out of what they have to offer. That's usually a pretty effective model in a space like the one they're in. If you'd like to help Base CRM help customers become 10x more productive, take a look at the company's Careers page. Right now they have some great opportunities including:
They also offer quite a few development jobs, but those are all in Poland.
Links to Help You Begin Your Research
What have you heard about Base CRM?
We've identified Base CRM as having career opportunities in the following categories:
|Restaurant Delivery
DoorDash Dulles, VA
|Food Delivery - Weekly Pay
Uber Eats Ashburn, VA
|Customer Service/Cashier/GSR- All Shifts
Nothing Bundt Cakes - Dulles, VA Sterling, VA
|Bid On Local Roofing Jobs - Reach New Customers In Your Area
HomeAdvisor Ashburn, VA
|Food Delivery - Weekly Pay
Uber Eats Sterling, VA


text:
Single Pagesite developed for Surrey based SDC
The goal of a website for the Surrey Dinner Club was to keep it super simple and produce a good-looking site that did just one thing very well. Inform visitors of the next meeting of the SDC: The location, date and time.
Our long time buddy and business coach Angus Lyon of Johnson Fellowes wanted to create a new type of Networking in Surrey. And so the Surrey Dinner Club was born. Angus is a strong BNI advocate and chairs the Woking BNI networking group. Angus and I have also attended sundry 4N meetings in Guildford, Woking, Farnham and other Surrey locations.
We recently wrote about the benefits of a One Page Websiteand this strategy was a perfect fit with Angus's objectives. More below on what constitutes a single or one page site.
The SDC site is a single webpage with 5 distinct pages: Home, About, Next Dinner, Team and Request Membership. However it is built in WordPress and loads as a single page. This single page structure means it is quick to jump to and from sections with no load time. It looks and operates extremely smoothly. The site has a neat slider section that provides key information in a scrolling way. It incorporates a Google Map that links to the next venue, links to Social Media for Facebook and Twitter feeds and a contact form.
The Surrey Diner Club site is now live and can be found at surrey-dinner-club.com


text:
Premieres: Monday, 17 August 9 PM (HK/SIN) | 8 PM (JKT/BKK)
Cast: So Ramphoum, Sarita Reth
A young couple move to their new home, and the wife starts to notice some strange and terrifying incidents. She also finds an old letter that reveals her husband's secretive past – just as he is paralyzed and a dark spirit threatens to take him. The wife tries her best to protect her husband, but will she succeed in saving him from the relentless angry spirit?


text:
You are here
Home >Case studies >Eau de Parfum CK Women
The Eau de Parfum Calvin Klein Women finds its inspiration in "a multifaceted concept of femininity." The work of art that is on the bottle cap was created by American artist Anne Collier. This black and white eye symbolizes "a look at the world around us."
The polished lazer-cut semi-circle hosts the eye thanks to a decor setting. The cap is made with Surlyn. These two pieces have been meticulously assembled to become one.
Albéa's XD11 Maximist reveals the woody floral notes of the Eau de Parfum.
The XD11 pump is manufactured in Albéa Le Tréport (Normandie, France), the group's global center of excellence for fragrance pumps, samples and mini-products. The original cap is made in Albéa Parigné (Le Mans), the group's center of excellence for fragrance caps.
No less than three of Albéa's expertises were needed to complete this perfume packaging.
Learn more about Albéa's XD11 pump : The XD11 pump is as delicate as it is solid thanks to its compact design and invisible engine (available in 70 µl, 100µl, 120µl). It protects the integrity of scents and ensures harmonious spray.


text:
The Miami Showmen's Association is located in Ft. Lauderdale, Florida. The MSA is a social and charitable organization for all of those in the outdoor amusement industry and their associations.
Mission Statement
To promote friendship and fellowship of all outdoor showpeople and their associates. To Perpetuate itself as a social and charitable organization.
Host your Event at the MSA Carousel Pavilion
Our banquet facility, the Carousel Pavilion is available for event rentals. The Carousel Pavilion is the perfect choice to host birthday parties, weddings, anniversaries, bar mitzvahs, family gatherings, religious services, business meetings, training seminars and classes, fitness classes, and much more. Our banquet style seating is capable of seating between 50-160 guests or up to 20 guests in a seminar seating arrangement.
For more information about hosting your event at the MSA Carousel Pavilion, click here.


text:
09/28/2018 / By JD Heyes
Though the concept of "global warming" has frequently been revealed to be little more than a Left-wing environmental hoax, so-called climate change – that is, the normal phenomenon of the Earth's ever-changing climate patterns – is very much a real thing (not the "human-caused" version; the normal process of changing climate, which occurs generally over the course of many years).
Changing climate also means changing weather patterns, with some parts of the world getting warmer, other parts cooler, some parts getting too much moisture, as other parts deal with abnormally dry conditions. In our own country, parts of the United States have been inundated with moisture, while other parts have been suffering through major periods of drought.
One thing changing weather and climate patterns can most definitely affect is the production of food and the sustainability of livestock in the raising of food. Warmer, dryer areas suffer from crop destruction and lack of grass and feed for cattle, goats, chickens and other organic food sources. Multi-year famines have occurred frequently throughout our history, and even though the U.S. is a rich country, given certain circumstances, famine could certainly happen here, too.
It's best to learn how to deal with famine now, before you have to worry about it for real. Here are nine ways to survive a famine. (h/t to SurvivalLife.com)
Sponsored solution from the Health Ranger Store: Lab-verified Nascent Iodine solution is a dietary supplement that provides your body with supplemental iodine to help protect your thyroid during radiation exposure. Nuclear accidents such as Fukushima (or nuclear war) can expose your body to radioactive iodine-131, a dangerous radioisotope. Pre-loading your system with stable iodine occupies the iodine receptor sites on your organs, causing your body to naturally expel radioactive iodine you may have been exposed to through air, food, water or milk products. This defensive strategy is recommended by nearly all health authorities, worldwide, including the Nuclear Regulatory Commission. Discover more at this link.
J.D. Heyes is a senior writer for NaturalNews.com and NewsTarget.com, as well as editor of The National Sentinel.
Tagged Under: bugout, Collapse, famine, preparedness, prepper, prepping, SHTF, survival, Survival Tips


text:
Sponsored By
Denver Police Investigate Shooting With 2 Victims Near 14th & YosemitePolice in Denver investigated a shooting that involved two victims on Friday night. The shooting happened near 14th and Yosemite.
Thornton Police Search For Driver Of Stolen Car Involved In Road Rage ShootingThornton police are looking for the driver involved in a road rage shooting Friday night.
Some LoDo Business Owners Leery Of Nighttime ViolenceA spree of recent shootings, stabbings and other violent crimes has business owners in the area of 15th and Market Streets in Denver's Lower Downtown nervous.
Denver Police Work To Confiscate Illegal Guns After 'Cowardly' ShootingDenver's police chief encouraged his officers to continue confiscating illegal guns after a shooting downtown early Sunday morning that left one dead and four others shot.
1 Killed, 4 Others Hurt In Downtown ShootingOne person died and four others were hurt in an early morning shooting in downtown Denver.
Lakewood Man In Critical Condition After ShootingOne man is in critical condition after a shooting near 4th and Sheridan in Lakewood.
Shooting Erupts Inside Chipotle During Property Sale Gone WrongCommerce City police responded to a Chipotle on Dahlia Street after someone reportedly opened fire inside.
1 Dead After Shooting, Police Search For GunmanPolice in Aurora are searching for the person who shot several people on Sunday afternoon, killing one.
Multiple Fatalities Reported At 'Mass Shooting' At Madden 19 TournamentThe sheriff's office in Jacksonville, Florida, said there was a mass shooting at a downtown marketplace on Sunday and urged people to stay far away from the area.
Clerk Shot During Attempted Robbery, Teen Suspects Ran OffA convenience store clerk is expected to recover after a shooting during an attempted robbery on Thursday morning.
Police: Man Shot Neighbors, Then Himself In Fight Over Dog PoopThree people are in the hospital after a dispute over dog poop turned into a violent shootout and standoff with police.
Mom Grieves From Hospital Bed Online: 'Every Time I Close My Eyes, I See My Babies; I See Them Dead'Amanda Simpson says her ex-husband shot her, their three children and her current boyfriend -- before shooting himself.
10-Year-Old Girl Takes Bullet While Shielding Baby BrotherA 10-year-old girl in Las Vegas is being called a hero after she used her own body as a shield to save her baby brother during a parking lot gun battle.
YouTube Shooter Told Family Members She 'Hated' The CompanyA woman who believed she was being suppressed by YouTube and told her family members she "hated" the company opened fire at the company's headquarters in California, wounding three people before killing herself, police said.
One Dead After Shooting In AuroraOne man died from a gunshot wound in Aurora Friday evening.


text:
@Karendipitee shared this photo of her Picasso bunny with me so that he can live on in infamy even after being consumed*. Isn't that what we all want for our lives? Anyway, I think this guy had a part-time job as a flounder. Or maybe some dude in the chocolate factory eye applying section was on crack. But I prefer to think that Mr. Bunny had an alter ego as a bottom dwelling fish. Because that makes more sense, right?
Here's hoping this bunny was one of a kind, otherwise some kid will probably be scarred for life on Easter morning.
*I'm told that he was delicious.
Help support LATR by using the links below when you shop:
Dog people: Click here to shop at Clean Run!


text:
A Bicycle Ride in Yorkshire
Only 1 left in stock
Product Description
In July 2014 the greatest cycle race in the world visits God's Own County of Yorkshire., The 101st edition of Le Tour de France starts in Leeds and spends two days cycling over 240 miles through the county., Inspired by Yorkshire's wonderful landscape and history A Bicycle Ride in Yorkshire is an illustrated guide to the route of Le Tour Yorkshire, by cyclist, writer and artist Heather Dawe., It is a guide to riding the route, to the sights and landscape seen along the way and to the rich cycling heritage found on Yorkshire's roads., Beautifully illustrated with Heather's paintings, the route is brought to life. On the first day Le Tour departs from Leeds into the rolling scenery of the Yorkshire Dales, past the breweries of Masham to finish in the spa town of Harrogate. Day two begins in the historic city of York, from where it explores the industrial valleys of the South Pennines, skirting the Peak District before finishing in the steel city of Sheffield., A Bicycle Ride in Yorkshire is an essential read for any cycling, Tour de France or Yorkshire aficionado., Heather Dawe has a passion for cycling, running and climbing in wild places. She is an endurance athlete who won the 2007 Helvellyn Triathlon, has finished first lady in the Fred Whitton Challenge twice and, in 2008, won the renowned Three Peaks Cyclo-Cross. As a runner she holds the female record for the Old County Tops fell race, has won at Elite level in many mountain marathons including the LAMM and the OMM, and has completed the Bob Graham Round in under 22 hours. She has lived in Yorkshire for 20 years. Spending her days working as a statistician and systems architect, she lives with her partner and their young daughter. Her passion for wild places extends to landscape painting and writing. A talented artist, her work has been exhibited in numerous places. A Bicycle Ride in Yorkshire is her second book, her first being Adventures in Mind, published in May 2013.
Additional Information
|220 g
Heather Dawe
Camp / Hike
Books & Maps
Walking & Travel Guides


text:
Sweeping Service in Phoenix & Surrounding Areas
Your Phoenix Area Sweeping Services Company
Agua Trucks, LLC
8525 N 132nd Drive
El Mirage, AZ 85335
Agua Trucks, Inc. is a family owned and operated company that has offered street sweeping, parking lot sweeping, construction sweeping and water truck service for municipalities, HOA's, property managers, and contractors in the Phoenix area since 2006. The key to our outstanding customer service is a fleet of exceptionally well maintained mechanical broom sweepers, vacuum sweepers, and regenerative air sweepers manned by operators with over 80 hours of professional training in sweeping best practices. Fully licensed and insured, the team at Agua Trucks stands ready to tackle your dust, dirt, and debris night and day, 365 days a year. From ordinary parking lot debris and litter to sand, gravel, paving millings, and construction site track-out, we can help. Whether your property, municipality, or construction site needs a one-time cleanup, an emergency sweep, or regularly scheduled power sweeping service, we have the equipment and skilled personnel to meet your needs and budget.
- City and County Municipal Street Sweeping
- Commercial and Residential Development / HOA Sweeping
- Erosion/Sediment Control
- Retail Shopping Center, Mall, and Office Parking Lot Sweeping
- Road & General Construction Sweeping for Phoenix Area Contractors
- State Highway and Interstate Sweeping
- Storm Water Pollution Prevention (SWPP) / Best Management Practices (BMP)
- Water Truck Service


text:
- Aara
- Akhlys
- Aoratos
- Archgoat
- Arkona
- Au Champ Des Morts
- Aversio Humanitatis
- Behexen
- Bestia Arcana
- Blut Aus Nord
- Crimson Moon
- Cultus Profano
- Draugnim
- Dødsengel
- Eryn Non Dae.
- Hegemone
- In The Woods...
- Inferno
- Infestus
- Kaleikr
- Latitudes
- Lychgate
- Manes
- Outre
- Pure Wrath
- Selbst
- Sühnopfer
- Tenebrae In Perpetuum
- Terra Tenebrosa
- TheNightTimeProject
- Throane
- Ulcerate
- White Ward
- Yerûšelem
WALLACHIA - New Album Details Unveiled
Norwegian legendary band WALLACHIA are back six long years after the critically acclaimed "Shunya" with a highly anticipated new album, undoubtely their most grandiose release to date, entitled "Monumental Heresy".
This fourth offering is a continuation of the rich hybrid experimentation of last full-length and a simultaneous backward glance towards the harshly beautiful atmosphere of the band's earlier works... never before have WALLACHIA pushed songcraft or the dynamics of their sound as far.
To keep you waiting, we unveil "Heathen Shores", a majestic first excerpt taken from this epic record :
Mastermind Lars Stavdal comments :
"Heathen Shores is the first excerpt we present from the brand new WALLACHIA album. This 9-minute majestic track really sums up the sound and musical vision of WALLACHIA perfectly. The track opens with the intro that was arranged and performed by Athelstan from the UK band FOREFATHER. Further into the song we also introduce the 4-piece male choir with guests from France and Austria doing an amazing job by enhancing the song's already epic feeling.
The song itself is a tribute to our home surroundings and the timelessness of the rough Norwegian landscapes, standing untouched and firmly representing our Heathen heritage. The wilderness is our source of spirituality and reflection - to whom we give our reverence. And we feel that musically and lyrically this song delivers a raw, majestic and Norse vibe from the first note until the last."
He adds :
"With this album we take one step forwards with the orchestral elements that was present on the previous album, Shunya, and at the same time we are much closer to our early days and the more savage approach, with a lot of dual vocal parts giving it some extra punch. Heathen Shores is a good representative for our sound as whole on the 4th opus Monumental Heresy."
"Monumental Heresy" contains 8 immersive and epic compositions :
1. Heathen Shores
2. So We Walk Alone
3. The Prophets Of Our Time
4. Silenced No Longer
5. The Parallel Fate Of Dreams
6. Beasts Of The Earth
7. Returned Favor Of Abandonment
8. Untruthology Abolished
The magnificent artwork was crafted by talented Artist Lucile Deadmunchst and the layout put together by Céline Bouvier.
"Monumental Heresy", scheduled for release on April 13th on Debemur Morti Productions, is available for preorder right now on Gatefold CD and Digital.


text:
Share on ThriftyFunThis page contains the following solutions. Have something to add? Please share your solution!
There are three main threats to the longevity of audio and video tapes: magnetic fields, moisture, and heat. The tapes are a magnetic media so be careful that they don't come in close contact with household electronics that have a magnetic field. Stereo speakers and TVs both have magnetic fields. You will also want to keep your tapes dry and between 60 and 70 degrees F. If you have a tape that is cold, let it warm up to room temperature before using it.
I am a specialist and assure that this little known idea can and has saved many a priceless and irreplaceable tapes.
Videotapes and cassette tapes that have been stored for a long time, and not played should always be rewound and fast-forwarded once before playing to loosen the reels and prevent stretching or breaking in the machine.


text:
First performed in 1666, The Misanthrope spotlights the absurdities of social and literary pretention. The play shows us a man who is quick to criticise the insincerities, inconsistencies and errors of others, yet remains blind to his own. As "the misanthrope" grows more and more irritable with others, the play becomes increasingly entertaining, even as a happy ending for the hero seems less and less likely.
This session will be facilitated by Manchester-based writer, producer and director Lucia Cox.


text:
Are you looking for some safe, all-natural fast energy boosters to perk up your day? Read on to find out more.
Your sense of smell is described as one of the most primal of senses, as your olfactory system is tied directly to your brain's emotional processing center.
For other senses, like sight and touch, visual or sensory input goes to your brain's main "switchboard," the thalamus.
Not so for scents, which wind their way through your nose and into brain regions responsible for emotions and memory, before they wind up in the thalamus.
Psychologist Johan Lundstrom, PhD, a faculty member at the Monell Chemical Senses Center in Philadelphia, explained it this way to the American Psychological Association:
"For olfaction, you have all this basic processing before you have conscious awareness of the odor."
This is one reason why certain odors – the scent of your mom's apple pie, freshly cut grass, the ocean – can quickly bring up powerful emotions and nostalgic memories.
It also explains why aromatherapy – the use of aromatic essential oils to enhance your physical, psychological and spiritual well-being – is so effective.
Whether you want to relieve stress, boost your mood, sleep better or enhance your energy level, there's an essential oil that can help. The scents that follow are particularly beneficial for enhancing energy; they'll even give your cup of coffee a run for its money.
Want to Know what are the Natural Fast Energy Boosters? Try These 5 Scents
- Jasmine
Jasmine is another uplifting oil with a pleasant floral scent. Research published in the journal Natural Product Communications revealed that jasmine essential oil led to increased autonomic arousal (such as increased breathing rate and blood oxygen saturation) and was associated with feelings of increased alertness and vigor.
In this case the oil was used as part of a massage oil. Researchers noted:
" … our results demonstrated the stimulating/activating effect of jasmine oil and provide evidence for its use in aromatherapy for the relief of depression and uplifting mood in humans."
- Peppermint
The scent of peppermint is crisp and energizing. Research shows peppermint essential oil has stimulant-like effects, so take a whiff whenever you desire increased alertness, mental clarity and invigoration.
- Sandalwood
Sandalwood has a woody, sweet aroma that's known to uplift the mind in a calming manner, allowing you to gain mental clarity and focus. It has anti-depressant properties and is valued for relieving mental fatigue.
- Black Pepper
Black pepper essential oil has warming qualities that are at the same time invigorating. Often used to enhance mental clarity, black pepper is the perfect scent for anyone with an active lifestyle. It works particularly well when combined with other scents, as it helps to enhance other oil aromas.
One study published in the Japanese Journal of Pharmacology even found inhaling essential oils including pepper led to a 1.5- to 2.5-fold increase in sympathetic nervous system activity and a 1.7-fold increase in blood adrenaline concentration compared with the participants' resting state.
- Lemon
The scent of lemon essential oil is invigorating and uplifting. It's often added to natural cleaning products because it has cleansing properties, but it works just as well for brightening your state of mind. Other citrus essential oils, like grapefruit, orange and lime, may also have energizing effects.
As for how to use essential oils for aromatherapy, there are endless options. You can, for instance:
- Put a few drops of oil into a room diffuser
- Blend it with water in a misting bottle and use it as a room or fabric freshener
- Put a few drops on a cotton ball and keep it in your pocket
- Sniff it right out of the bottle
- Add it to your bath water
- Add a few drops to unscented body lotion or massage oil
In aromatherapy, a combination of oils is often used to provide a synergistic effect that's even more powerful than the use of one essential oil scent alone.
If you need some more ideas about fast energy boosters, click and watch the following 2 videos:
Are you interested in getting healthy or living a more natural life? Click HERE
By Jesse Cannone – Creator of the International Best-Selling Back Pain Treatment Program "Lose the Back Pain System" and Best-Selling Book "The 7 Day Back Pain Cure"
Unlike most treatments which only deliver temporary relief, if any at all, muscle balance therapy delivers lasting relief to 8 out of 10 people who use it because it addresses the underlying cause of the pain, not just the symptoms.
If you are suffering from any type of back pain, neck pain or sciatica, I urge you to learn more about this breakthrough new treatment. Click HERE to learn more