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Very Veggie Enchiladas with Homemade Sweet Potato Tortillas
Team: Sharon Shredders
Host School: Sharon Elementary
Age Group: Middle School
3 tbs. Olive oil
1 red onion- diced local
4 cloves garlic- diced local
1 red pepper chopped
I head broccoli- chopped small
4 carrots- peeled and shredded local
1 bag spinach-wash local
Enchilada Sauce
3 tbs. Olive oil
3 cloves garlic- minced local
3 large tomatoes, diced local hydroponic
1 tbsp. Chili powder
1 tsp. Cumin
½ tsp onion powder
½ tsp. Oregano
1 tsp. Salt
½ tsp. Black pepper
3 tbsp. Tomato paste
1 ½ c veggie broth
1 tbs. apple cider vinegar
Sweet potato tortillas- makes 20 tortillas
2 cups flour local
2 cups mashed sweet potatoes (about 2 medium sweet potatoes, peeled and diced) local
1 tsp. Salt
2 cups grated cheddar local
½ cup sour cream mixed with water to become drizzly local
2 tsp. siracha
½ red onion diced finely local
½ cup cilantro diced
Preparation Instructions
Directions for Veggies
Chop red onion, garlic, red pepper, and broccoli
Peel and shred carrots
In a large saute pan, heat olive oil over medium heat
Sautee onion, pepper and garlic for about 5 min until softened
Add broccoli and saute 5 min more
Add spinach and carrots, sautee 5 more min.
Directions for enchilada sauce
In a large skillet, heat olive oil on medium heat. Add in fresh tomatoes and garlic. Saute for 5 minute.
Add in tomato paste, broth, vinegar and spices continue to cook for 5 more minutes
Use an immersion blender and blend together until smooth
Directions for sweet potato tortillas
Fill a medium pot with hot water and salt, heat to a boil.
Peel and dice sweet potatoes into small cubes
Add sweet potatoes to water and boil until soft enough to mash, about 10 min.
Drain sweet potatoes and mash
Combine flour and salt together in a bowl
Add cooked sweet potato and mix with flour until flour is completely absorbed. Knead and work it using your fingers until combined and forms a ball
If it seems too wet or sticky, add and mix a little extra flour
Allow dough to rest for 5 min.
Dust a flat surface with flour and shape dough into a long log
Divide dough into 18-20 portions
Roll each portion into a ball and sprinkle with flour
Roll each portion out until it is ⅛ inch thick
Heat a non-stick pan/ griddle. Spray with olive oil. Spray the top of each tortilla with olive oil. Heat tortillas for 1-2 min on each side until slightly browned. Stack on a plate and cover with a towel to keep warm and flexible
Putting the enchiladas together
Add a 1 cup of grated cheese and ½ cup enchilada sauce to the veggie mixture- stir to combine
Add enchilada sauce to the bottom of a baking dish to coat the bottom
Lay a sweet potato tortilla on a flat surface and place a scoop of veggie filling in the middle of tortilla. Roll up each tortilla and place in the baking dish on top of the enchilada sauce. Do this until the pan is full.
Smother in enchilada sauce and remaining cheese until evenly covered
Bake at 375 for 15 min or until cheese is melted and sauce begins to bubble
Remove from baking dish to serve and drizzle with sriracha sour cream, diced red onion and cilantro


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It isn't the holidays if you aren't accessorizing properly. What better way to celebrate the holidays than with some penguins on your ears? Deck your ears with Christmas jolly thanks to this 9 piece value set. For any holiday related accessories, from bangles to necklaces, Claire's has you covered. Get holiday glam-ed up by clicking here.
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Photo: Claire's


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Table 87 Frozen LLC Recalls Pork Pizza Products
Table 87 Frozen, LLC., a Brooklyn, N.Y. firm, is recalling approximately 649 pounds of frozen pizza products that contain pork that were produced without the benefit of federal inspection, the U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) announced today.
The frozen prosciutto pizza products were produced from April 1, 2019 through June 4, 2019. The following products are subject to recall...
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McCann Worldgroup's Cathay Pacific team scooped Hong Kong's most prestigious marketing awards with their innovative campaign around Betsy, a craft beer with altitude
Spare a thought for the poor airline chefs next time you're groaning over an unappetizing offer of chicken or fish at 35,000 feet. Most of us are unaware of the effort that goes into countering the impact of altitude, humidity, noise and cabin pressure on passengers' palates – which can be impaired by up to 30 percent.
While some airlines try to adapt their food recipes to perk up the tastebuds, the team at McCann Worldgroup Hong Kong went a step further and brewed up a unique idea for their client Cathay Pacific: the world's first beer tailored to taste best after the fasten seatbelt signs are turned off. So Betsy Beer, named after Cathay's first aircraft, a 1946 DC-3, bubbled her way into existence.
As McCann Executive Creative Director Martin Lever explains, this wasn't simply a way of improving the dining experience of first- and business-class passengers by tapping into the surging global popularity of craft beers.
"In the airline world, there's a problem of perceived product parity. Innovative initiatives and engaging storytelling can help brands rise above this problem," Lever says. "That's exactly what Betsy Beer was designed to do."
With help from a team of expert brewers, Betsy was tweaked for high-altitude. She's about 10 percent fizzier than her sea-level rivals, adding what's known in the food and beverage trade as "mouthfeel." Betsy's made from wheat and is unfiltered, which makes her tastier – and with higher levels of vitamin B to help kick that jaded feeling after a long-haul flight.
They combined Hong Kong honey and dragon fruit with Fuggle hops from Britain to marry flavors from Cathay's home base with the beer's U.K. launch destination.
Betsy proved an immediate hit with Cathay's customers: the initial batch of 4,800 bottles ballooned to more than 50,000 after just one month.
Not a bad entree into the craft brewing world. And from a marketing standpoint, the returns were staggering. McCann estimates the beer generated media coverage worth more than 100 times the campaign budget, reaching an audience of more than half a billion people. The return from spending on advertising was also about 100 times, the company says. Crucially, three-quarters of customers surveyed said the beer enhanced the Cathay experience.
The campaign won McCann Worldgroup Hong Kong the Grand Kam Fan award at the HK4As – the Association of Accredited Advertising Agencies in Hong Kong – late last year, as well as Kam Fan and a Gold in the Design & Crafts category. All of which garnered their client the prestigious Client of the Year title.
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Big Fish, Biggest Pond
Cracking the China conundrum: how to magnify your voice in the world's biggest marketplace
It's a problem every international brand faces in China's massive market: how to get your name known, let alone talked about and valued. Fragmented, vast, often insular: Traditional marketing strategies can mean tossing bundles of money into a bottomless well.
When Cathay Pacific wanted to integrate and rebrand its Dragonair unit as Cathay Dragon its marketing team at McCann Worldgroup knew which way they had to turn, explains Executive Creative Director Martin Lever. "There's only one way to capture imaginations across the whole of China if you have a minimal budget: think social!"
They found three engaged-to-be-married travel influencers from China's top three social travel platforms and got them to compete for a chance to be married on a Cathay Dragon flight, have a reception in Cathay's First Class lounge, and a honeymoon trip with Cathay Pacific. Then they invited the whole of China to take part through voting and user-generated content.
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"By having a big emotive idea strategically amplified by the right social influencers across relevant platforms, we were able to ignite a massive conversation – and drive the levels of brand and product awareness that traditional advertising channels would have struggled to deliver," says Lever.


text:
Hi friends! I'm finally back with another Talk With!!
In case you missed it, "A Talk With" is a brand new feature here on Drizzle & Hurricane Books, where I will invite young adult book authors to chat with me about their upcoming books, share their writing tips and tricks and more!
I'm having today another wonderful debut author, Isabel Sterling! Her upcoming book, These Witches Don't Burn, is one of these queer witches stories that we need more of and that sound just way too good to pass up on. Let's get on with this lovely interview, where we talk about her debut, creating magical worlds, juggling between plot and characters and so on!
1. Can you describe your debut, These Witches Don't Burn, only using three words?
Thank you so much for having me! Ooh, this is tricky. If I could do a three-word phrase, I'd say TWDB is "queer witchy fun!"
2. Are there any real-life places (or / and imagined) that inspired you for These Witches Don't Burn? Any songs, a particular playlist or some books/tv shows that inspired you as you wrote or before, to write about witches?
I've been obsessed with witches my entire life. I grew up on movies like Practical Magic, The Craft, and Hocus Pocus and read every kind of witchy book I could get my hands on. I remember ordering all the T*Witches books (by H.B. Gilmour) from those book fair fliers we got at school, and I read dozens of books in the Sweep series by Cate Tiernan as a young teen. I also watched a lot of Charmed (and am currently loving the reboot!) and very much wanted to get my Hogwarts letter.
When I first drafted TWDB, I had never been to Salem, MA — though I had wanted to visit since we studied The Crucible by Arthur Miller in high school. In 2015, after I'd finished the first draft, I was lucky enough to visit Salem with my wife (who was a new girlfriend at the time), which helped develop the setting for the novel.
3. How did the magic system of These Witches Don't Burn come to be and how did you polish it? Do you have any tips for us writers to create a magic system? Do you take particular notes for each scenes where it is used, how it all works out, like a sort of, magic-system manual of some kind? 🙂
This is a great question! Believe it or not, the very first draft of TWDB didn't have any of the Witch Clans that are in the published book. When I was stuck in revisions, I realized I was bored with the story because it didn't have any magic. The system developed over the course of revisions and edits, but the initial inspiration came from sitting down one day and brainstorming all the things I loved about magic in the stories I grew up on. Ultimately, I settled on three types: Elemental Witches (heavily influenced by my love of Avatar: The Last Airbender), Caster Witches (your more typical potions/spells type of witch), and Blood Witches (the creepy witches the other two love to hate).
For other writers looking to create their own magic systems (and this is what works for me, but writing advice is never one-size-fits-all, so your experience may vary), it's important to keep logic in mind. Yes, we're creating our own rules, but if those rules don't make sense to the reader, they're likely to get lost and put down the book. You don't have to get it right on the first try (revision can be a great time to iron out the finer details and logical issues), but it's something to keep in mind.
4. These Witches Don't Burn sounds like a delicious mix of contemporary and character-focused story, in a magical world filled with intrigue, too: how did you manage to juggle between the magical, fantasy and thrilling aspects of it all and still keep a tight focus on your characters and their development, too? Did you write different outlines and character arcs and made them meet afterwards in your writing or edits?
This is another excellent question! It was definitely a balancing act to keep the mystery and tension going forward while creating space for more character-focused moments, like the aftermath of Hannah's breakup and exploring her new crush. Getting the balance right was definitely a collaborative process with my editor. My inclination as a writer is to race through the plot, and she helped me remember to slow down and give these exhausted teens a moment to catch their breath!
1. Are there any fellow #novel19s and debuts that you really loved and would like everyone to read?
Gosh, so many! I haven't read it yet, but I'm so excited for Kat Cho's Wicked Fox (the ARC is waiting for me on my kindle!) and Hot Dog Girl by Jennifer Dugan is a really fun queer romcom.
2. If you woke up with magical powers just like the ones in These Witches Don't Burn, which powers would you like to have?
I would love to be an Elemental Witch. Having the power to manipulate the elements would be really cool, and I've always felt an affinity with water. Plus, ever since I watched Practical Magic, I've been desperate for the power to light candles with magic.
3. Can you share one line of These Witches Don't Burn with us?
I think readers expect the fun magic, ex-girlfriend drama, and awkward flirting (which are all parts I love!), but the book is also a bit dark at times, so I present you with the end of Chapter 2:
"Fire flickers a few yards away, but what captures my attention is the girl on the ground. Covered in blood."
Thank you so, SO much for taking the time to chat with me about your upcoming book, Isabel! Friends, I hope this made you want to pick up this wonderful book as soon as you can, it releases on May 28th!
📖 More about These Witches Don't Burn
Buy on Amazon | Barnes & Noble | Book Depository
Hannah's a witch, but not the kind you're thinking of. She's the real deal, an Elemental with the power to control fire, earth, water, and air. But even though she lives in Salem, Massachusetts, her magic is a secret she has to keep to herself. If she's ever caught using it in front of a Reg (read: non-witch), she could lose it. For good. So, Hannah spends most of her time avoiding her ex-girlfriend (and fellow Elemental Witch) Veronica, hanging out with her best friend, and working at the Fly by Night Cauldron selling candles and crystals to tourists, goths, and local Wiccans.
But dealing with her ex is the least of Hannah's concerns when a terrifying blood ritual interrupts the end-of-school-year bonfire. Evidence of dark magic begins to appear all over Salem, and Hannah's sure it's the work of a deadly Blood Witch. The issue is, her coven is less than convinced, forcing Hannah to team up with the last person she wants to see: Veronica.
While the pair attempt to smoke out the Blood Witch at a house party, Hannah meets Morgan, a cute new ballerina in town. But trying to date amid a supernatural crisis is easier said than done, and Hannah will have to test the limits of her power if she's going to save her coven and get the girl, especially when the attacks on Salem's witches become deadlier by the day.
🎁 Preorder These Witches Don't Burn
If you preorder These Witches Don't Burn (or request it at your local library), you can receive these lovely and exclusive vinyl character stickers!
This campaign is open internationally and until June, 4th. More information here.
📖 More about the author, Isabel Sterling
Isabel Sterling has been dreaming up new worlds since childhood, but she didn't commit any of them to paper until after college. When she's not writing about magic and murder, Isabel can be found lost in a good book, binge-watching her favorite shows, or swimming in the lake. She lives in central NY with her wife and their brood of furry children: cats Oliver and December and a mischievous puppy named Lily.
Her debut YA novel, These Witches Don't Burn, will publish in May 28, 2019 from Razorbill.
Find Isabel Sterling on:
💻 Website 📱 Twitter 📷 Instagram
Do you want to read These Witches Don't Burn? Did you read it already?
What are some of your favorite books with witches? Let me know your thoughts and recommendations in comments!


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Power of Love
One of my favorite artists, Mike Farris, sings Power of Love.
Posted on 01/07/2018, in music and tagged Mike Farris, music, Power of Love. Bookmark the permalink. 7 Comments.
Posted by Xena
Posted on 01/07/2018, in music and tagged Mike Farris, music, Power of Love. Bookmark the permalink. 7 Comments.
"Healing power of love"
"Power of Love,
Burning down deep, comes from Above"
– Oh, yeah, I love this song.
Thanks for introducing me to this song and Mike Farris, Xena 🙂
You are most welcomed. You might remember other songs by Mike Farris, such as "I Can Use A Little Mercy Now." (((((Hugs)))))
Oh, yes, I do, and I remember you also posted it on your blog. I just listened again to it on Youtube. It is powerful!
Actually, you posted it in the comment section on a post about Mike and his music. 🙂 I remember you enjoyed it. Yes, Mike sings songs with lyrics that are powerful, even when he gospels up R&B songs.
Oh, thanks for refreshing my memory.
I am at the five minute mark on this right now:
Wow, I've never heard of him before. " Mike Farris is an American musician. He was the founder and lead singer of Nashville, Tennessee group Screamin' Cheetah Wheelies, but has been a solo act since 2001″ I do vaguely remember the Screamin' Cheetah Wheelies.
I think that Mike is one of those performers who deserves more attention. I love his singing style and can listen to him for hours.
© Blackbutterfly7, 2012 – 2019. Unauthorized use and/or duplication of this material without express and written permission from this blog's administrator is strictly prohibited. The reblog function that Word Press provides is considered consent. Comments are owned by the commenters who are free to post the same elsewhere. For all others, this copyright notice includes comments.


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elevate your event.
Based in Wellington NZ, Society Entertainment is the lower North Islands' premier entertainment company. Providing world class dancers and thrilling pre-choreographed routines, perfectly suited to your events theme - Society Entertainment will leave your guests with something to talk about, and ensure that - whatever the event - it's one to remember.
We have packages to suit all budgets. Choose from our repertoire of prepared performances, or let us customise your event entertainment from scratch, the options are endless!
With a background in Ballet, Jazz, Modern, Contemporary, Irish, Highland, Commercial and Ethnic dance styles - Mila was a dancer for 17 years before gaining her qualifications as a Dance Teacher (AIDT Associate member) and has been working as a teacher and choreographer for 18 years.
Having founded Inzpire Dance Company in the Bay of Plenty in 2008, she enjoyed 11 successful years working with dancers young and old, and especially enjoying creating works for community, sport and corporate events over the years.
Choreography Credits include:
Co-choreography of Tauranga Musical Theater CATS 2009
Tauranga Moana Seafood Festival 2009 / 2010 / 2012
Audi Dealership Mount Maunganui LAUNCH 2009
Heart Children BOP Heart Stopper events (2010 and 2011)
Bayley's Realtors awards nights 2012 / 2014 / 2019
Tauranga City Council events
Wharf Street Festival
Disneyland 2015 performing arts programme
Tauranga Diwail festival 2015 / 2017
Tauranga Olympian Parade
Anna Hawkins Music video "Hallelujah"
BOP Rugby ITM CUP - 2011 to present (2019) current Bayley's dancers
WBOP Magics Dancers 2018 – 2019 season of the ANZ premiership
Sketchers Store Opening Tauranga Crossing 2019
Wellington Lantern Festival 2020
Mila has recently relocated to Wellington (July 2019) and can't wait to work with the talented dancers of the region, and to bring the power of the arts to game days, parades, and events throughout the city and beyond.
Renee has 14 years of dance experience with a strong technical background. She trained with the Institute of Dance in RAD Ballet and SADTA Modern Jazz.
In 2014 she joined Inzpire Dance Company and performed with the Bayleys Dancers for four consecutive seasons at the BOP Steamers ITM Cup matches, and other corporate and community events.
In 2015 Renee performed at Disneyland Anaheim with World Class Dance Tours and went back to complete the International Dance Academy (Hollywood) Certificate Programme in 2016. She is currently studying towards her Diploma in Performing Arts (Dance) which will be completed in November 2019.
Renee has had various performance experience including performances with DKCM, Deluxe Events and Beervana.
Renee is excited to begin curating event entertainment and providing opportunities for professional dancers and performers in New Zealand.


text:
Meanwhile, this report is mostly just progressive talking points that offers a wordy real news update near the end of the screed . . . Checkit:
Could Missouri women face felony charges for having abortions?
Officials in Missouri's public defender's office are worried women who seek self-managed, or pill abortions, could be subjected to felony charges. Associated Press file photo When Missouri Gov. Mike Parson signed into law one of the nation's most restrictive abortion bans in May, supporters of the measure said their aim was not to prosecute women who seek the procedure.


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Enable file versioning / history for all types of files (not just Office types)
I know you can recover previous versions of Microsoft Office files in OneDrive. But in Dropbox and Google Drive, you can recover previous versions of any file type (and this has been a long standing feature of these services). Please give us the ability to restore previous versions of any file type in OneDrive.
We are expanding version history support to all file types for OneDrive personal account users. As a result, customers will now be able to restore or download previous versions of all their files for up to 30 days. It will be available to everyone this summer; currently, approximately 10% of OneDrive users can see and interact with this feature.
You can read more about file versioning and how to use it on our blog: https://blogs.office.com/en-us/2017/07/19/expanding-onedrive-version-history-support-file-types
We understand how critical your data is to you. We also understand that threats are continually evolving and that our product must evolve too. This is one of several steps you will see us take in the near future to help protect you and your data.
262 comments
David Mackey commented
I didn't know about this lack of previous versions. Definitely gonna keep me as recommending OneDrive as a solution.
Teddy Svoronos commented
Microsoft has done great work integrating with iOS, way above Dropbox and Google drive, but this issue is preventing me fro switching over. Really hope this can be added soon.
Akhila commented
I did a big mistake by keeping my photos on onedrive. I lost all my photos. I should have used google drive. If you guys cannot provide us with orevious versions whats the point of advertising onedrive as a backup resort. We will go to our old method like hard drives or pen drives to keep our files. Because this service and pen drive is no different. Thank god we have google now. I was a microsoft biased person, but after this not any more.
D Mytty commented
"This work is in the plans" ... Is that like 'we should all plan for World Peace someday'? Or does Microsoft have a REAL plan, as in programmers assigned and boiling the code?
Considering that the short, not-to-the-point 'in the plans' comment was the most recent post by a Microsoft type, and it was made in March of 2015...it seems this might be more of the 'undercommunicate and underdeliver' strategy from Microsoft.
You do not offer this for onenote files!!!!!
Tyron commented
How come that hasn't seen light yet? It's the 5th top voted feature! I just feel I did a terrible mistake switching Dropbox for OneDrive...
Chris Miller commented
Our company just switched operation over to onedrive. Looking at how I can recover lost or corrupted files. All files have just one version because they have not been modified since the migration. So, I don't think a versioning based restore is going to work. I read that Microsoft does daily backups. How does one restore from these backups?
Is there a light at the end of the tunnel on the ETA to have versioning/history for all file types?
Not all Office file types are covered... Visio files are currently not covered by OneDrive
Taylor commented
It's a shame that onedrive is so far behind dropbox. The only reason I use it now is because it comes with my office 365 account. But I can surely tell for now: Dropxbox is MUCH better than onedrive.
Since I got onedrive, these things bother my life compared to when I used dropbox:
- Closing Word files takes suddenly time because it needs to upload. => What the heck? Dropbox can upload a file and does not waste my time while having a loading screen!
- Onedrive "upload errors" => So maaaaany times some files I really do not care about want my onedrive attention, because it seems onedrive cannot upload them. I mean seriously... I never have problems uploading a file with Dropbox...
Why is it so difficult to make a tool that WORKS? I mean seriously...
Would like to have this function also
Kind of a long plan. Suggestion has been made in 08-2014.
Already 1915 votes. Come on Microsoft. Step it up!
We need this now!
I'm seriously considering going to another provider since file versioning could have recovered some important data in my work. Such a disappointment considering other providers have this feature for other files.
Neal commented
I'm sticking with dropbox until versioning for all file types is provided. Versioning longer than 30 days would be a plus. I'm even okay if it counts against storage, so long as controls are provided to manage.
Ies Nijman commented
I indeed agree that this is a very important feature which for me is prohibitive as well. Additionally, not all the office files support this like advertised: Onenote, Visio and Project also lack versio history.
Rob Gilliam commented
You say this is available for "Office file types", however it appears that OneNote notebooks do not have versioning.
Christian commented
I installed Windows 10, which broke so I had to use my backup drive to go back to Windows 7. But I felt sure since my most important files were on OneDrive. However - since the most recent files were not present in my backup, OneDrive deleted them from the cloud as well, and restored others to an older version - data loss. That really really should not happen.
Linus Ahlemeyer commented
No doubt, this is very much needed. I just wanted to buy OneDrive for Business, but after learning this is missing I must consider working with DropBox until this is included. Please anyone from Microsoft let us in on a rough ETA.
Soonjae commented
It's the key feature you are missing...Frustrated!
Victor commented
top3 feature keeping me from changing from dropbox to onedrive


text: A clinical practice guideline for the diagnosis, treatment, and follow-up of oropharyngeal, hypopharyngeal and laryngeal cancers. The guideline examines preferred locoregional staging methods and treatment options including an emphasis on interprofessional care. Outcomes of interest include limited evidence for the benefits of surgical treatment in some cases and some evidence that primary chemotherapy may provide some benefit over radiotherapy.


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Maple Village Waldorf School Students recently participated in Algalita's POPS International Youth Summit, a 3-day empowerment experience held at the Ocean Institute. POPS is an acronym for Plastic Ocean Pollution Solutions.
Over 125 students across 8 countries and 6 U.S. states convened in Dana Point, CA to advance global solutions to plastic pollution. Maple Village Waldorf students won a place at the Summit based on their innovative ideas on how best to combat plastic pollution in their own community.
Ollie Hong, 12, Jermonie Billingsly, 12, and Sophie Smallie, 14, entered a project to replace single use plastic products at Long Beach food banks. They are reaching out to businesses that sell reusable bags and asking them to donate their misprinted bags to the food bank. The idea being that those coming to the food bank will use them and return with them on an ongoing basis.
Learn More at The Grunion
Photo: Maple Village Waldorf School
- Middle School
- Nature and Environment
- Society
- Waldorf Education


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Tag Archives: genetically modified
Are there genetically modified animals in the supermarket?
Animal breeders have used selective breeding, a form of directed evolution, since animals were first domesticated. Using this approach, individual animals are chosen to be parents of the next generation because they exhibit a desirable trait or
A covert authentication and security solution for GMOs
Significantly increasing worldwide utilization of genetically modified organisms (GMOs) presents challenges to ensure security, authenticity and validation of material goods and legal agreements. Similarly to the evolution witnessed in Internet protocols, strategic focus is required to anticipate,
GM herbicide-tolerant crops, weedkillers, and resistant weeds: overview and interplay
Since their beginnings, GM crops have been a subject of debate in spite of their quick adoption by farmers in several countries. In the last 20 years GM herbicide-tolerant (HT) crops, generally glyphosate-tolerant crops, have been the
Public attitudes to GM food
Public concern can often be a stumbling block to successful innovation, particularly when the underlying science breaks new ground. This does not just affect peoples' readiness to consume new products, but may also affect the regulatory environment.


text: doll accessories set (0) No Reviews yet $309.00 Quantity discounts available QuantityPrice Out of Stock quantity Details Equip the dolly's nursery with a dresser, cradle and stroller. Your child will be pleased to have all they need to tend their new doll. Item#: 5007Made In: Poland, USAMaterial: wood Helpful Info Includes rose stroller, dresser and cradle. Products You May Like Reviews 0 BackRatings & ReviewsNo reviews availableBe the first to Write a Review Customers also bought


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Finding innovative solutions for our customers transportation requirements is what keeps us focused every day. We combine forty five years of trucking experience with state of the art logistical support and a dedicated team of professionals to provide unparalleled service.
Never follow, always lead is the philosophy we embrace to ensure a bright future for our customers, our staff and our community. You could say we're here for the long haul.
Shadow Lines Transportation has had a consistent presence and experience in servicing the I-5 Corridor from Seattle, Tacoma and Portland to Vancouver for over 25 years.
We operate one of the largest fleets providing intermodal/container as well as flat deck services throughout this corridor to maximize and maintain our fleet size and ensure our commitment to service.
We operate a single operational division to handle the specific and detailed requirements that this service lane presents. We focus on communication and information validation to mitigate any challenges with cross border transportation.
Our current fleet consists of experienced and fully approved operators as well as a modern, diverse fleet of container chassis and flat decks. Current truck fleet is 37 operators with access to over 100 pieces of equipment – super chassis, tridem chassis as well as quad axle flat decks – to provide a consistent and quality service.
Our core operations team is well experienced and trained in the comprehensive requirements necessary to ensure a consistent flow both north and south across the Border, as well as within the ocean and rail terminals. Established communication protocols are in place to ensure strict awareness of our operators' whereabouts at all times. We have access to cross trained operational staff within other divisions to always ensure a consistent and seamless service experience.


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Saturday, November 13, 2010
I used to think I'm passionate about writing. But the more writers I meet, the more unsure of myself I feel. If writing really was my passion, how is it that I've written 11 pages of my so-called book some 2 months back and never touched it again? The summary of which I did another 6 months ago? Then maybe my book isn't it. Or maybe I don't have my story yet.
I feel confused. I feel I'm in a perpetual trance of everyday things. I truly love the people in my life. But maybe thats not all that I want. I love bombay. But I dont love any place for too long which makes me feel tied. I want to go to fucking italy. Please someone take me to italy with them.
Ok, stop, pause, everyone. Breathe. I'm making a new plan. It just came to me, while writing this. Fuck everything. I deserve every bit of happiness in this world. Now this is what I'm going to do. I just got a raise. So.. I'm going to save every month and by my next birthday, September, I'm going to leave this country. I've always, always wanted to live in different countries. If I don't do this now, I cant do this ever. I have to, I need to do this for myself.
I have to do this.
And now, I have to save - no more splurges. I have to think of a country which talks to me the most. I have to prepare.
I think I've finally nailed what I've been irritated about. I have a vision of myself living like something, somewhere and I cant wait to start it. But I'm waiting, without a date, I feel like I'm working towards a non-existent goal. My life and my purpose is blurry around all this waiting.
I don't anymore want to wait around for anything, anyone. I cant wait to start this life and I figure at 25, half of my life has already gone. More sad than happy. And who said, the balance has to be this off? The clock is ticking and I need to get going. Thats the only thing good for me. An unhappy me is an annoying me. An unhappy me is everything I don't want to be.
One wait leads to another wait, and before you know it, there is a whole checklist of your ducks that need to be in a row. If I can wait for someone and their plans, wait, what about my plans? I at least deserve a date. And I hate pushing for a date, people need to respect other people's dreams and dates..umm.. time I meant. Yes, people need to respect people's time.
I'm also really hoping rupee value goes up, oh and also, I'm hoping I get a fucking job in another country. WOW. That's a lot for me and my poor little bachelors of a degree.
I need to stop talking now. Wokay then.
6 comments:
- Hussain Haidry said...
We're pretty much in the same phase. Cheers. :(
- November 14, 2010 at 9:52 AM
- Sonia said...
Take a break and come to San Francisco! Also, there's this college http://itmitourtraining.com/index.shtml in SF, which might open doors to touristy things that pay ;-)
- November 14, 2010 at 3:43 PM
the last para was really a ramble..
all the best with the Italy plans. I will back-packing in Europe (incl Italy) sometime next year - If i dont have to wait anymore.
- November 16, 2010 at 12:44 AM
- அ. பசுபதி (தேவமைந்தன்) said...
You can be sure of yourself as a successful web writer. I too experienced the same difficulties. From 1968 in mags and for the past ten years in net, I write. Your writing is interesting; presentation nice. Kudos!
- Devamaindhan
- December 7, 2010 at 9:09 PM
- mahesh said...
hey i just love the way u write..
its so interesting..and specially this post..
may be bcoz e1 i am in the same phase..
- December 9, 2010 at 9:20 PM
- mahesh said...
hey i just love the way u write..
its so interesting..and specially this post..
may be bcoz e1 i am in the same phase..
- December 9, 2010 at 9:21 PM


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The Good Friday Walk has been an outreach ministry of Christ Church for decades. Parishioners, family and friends walk to raise awareness of the hungry and homeless on Long Island, to benefit The INN. Some parishioners and friends walk while others support their efforts by pledging donations to The INN.
Walkers will meet at Christ Church (33 Jefferson Street) and walk for approximately two hours.
For additional information please call Christ Church at 516-775-2626 or email firstname.lastname@example.com. Christ Church looks forward to combining with others for this worthwhile outreach ministry.


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Premier Preschool & Child Care in Powell, OH
The Compass School of Powell, Ohio opened in 1999 and was an early adopter in central Ohio of the Reggio-Inspired approach to early childhood education. In 2010, our Powell school earned NAEYC accreditation, which has been consistently maintained since that time. NAEYC accreditation is arguably the most recognized and respected benchmark of quality for early childhood education programs.
Reggio-Inspired Education & Child Care in Powell, OH
The Compass School of Powell, Ohio has a three-acre campus with an expansive playground. The school houses twelve classrooms supporting the following programs for children from six week to 12 years of age:
The Reggio Emilia approach to education has been at the forefront of early childhood education for over 40 years (Rinaldi, C. 2006. In dialogue with Reggio Emilia: Listening, researching and learning). This philosophy is rooted in a strong image of children as capable, rich in potential, and full of interests and ideas about the world around them. Teachers at The Compass School seek to inspire learning by encouraging creative exploration and collaboration between children in a welcoming, community-based school environment. For more information about what it means to be 'Reggio-Inspired,' please click here.
After School & Summer Camp in Powell, OH
The Compass School of Powell offers innovative and child-centered early education programs serving families in Powell, Dublin, Lewis Center, Worthington, and the Greater Columbus area. For school-age programs, The Compass School of Powell currently offers transportation to and from select Olentangy and Dublin School District schools, including Indian Springs, Scioto Ridge, Wyandot Run, Tyler Run, Liberty Tree, and Chapman Elementary Schools.
The Powell campus is conveniently located at 3989 North Hampton Drive, just off Sawmill Parkway and 2.5 miles north of I-270. The school is also just a five-minute drive from the Columbus Zoo and Aquarium. Click here for directions from your location. We look forward to showing you our beautiful school! Call us at 614-764-8844 to schedule your personal tour.
Tara Aledia – Executive Director
Tara has ten years of work experience in the early childhood field, and began as an administrator at The Compass School in 2010. She holds a Bachelor of Arts degree from York University (Toronto) majoring in Psychology, as well as an Early Childhood Education diploma from Humber College (Ontario, Canada).
What Powell Parents Have to Say


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The Induction Programme for the Undergraduate freshers were conducted in two days – 27 June and 01 July 2019. Principal Dr.C. Saidalavi inaugurated the event. An ice breaking session handled by Mr. Shabeermon and Mr. Noushadali was followed by sessions on anti-ragging and student conduct by Ms. Aswathi M. P, the student welfare dean, student union activities by Ms. Rabsha & Rifa Odakkal and Fine Arts by Mr. Haris. The various clubs, cells and programmes were introduced by the respective coordinators – SSP by Ms. Poornima, NSS and WWS by Ms. Shameema and Dr. Usman, NCC by Capt. Dr. Meeradevi, Scholarships by Mr. P. Muhammedali, Sports club By Dr. Zakir Ahmed, Tourism club by Ms. Haseena Jasmine, Entrepreneurship Development Club by Ms. Fathima Shajitha, Reader's forum by Mr. P. Sidhique and Science Club by Mr. Shihabul Haq.
A demonstration session on Taekwondo was handled by trainer Mr. Sudheesh and team. A session on women and career was delivered by an external resource person, Mr. Saidalavi, Asst. Professor of History from MES College, Ponnani. IQAC coordinator Ms. Annie Ninan spoke to the students about the quality maintenance in the college. A yoga practice session was handled by Dr. Zakir Ahmed, head of the Dept. of Physical Education. A feedback session followed and Ms. Sangeeta K, coordinator of the course delivered the official thanks note.


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Dear Michael,
We would like to take this opportunity to thank you for the very efficient and professional way that you have handled our case. You have been very pleasant, courteous and helpful in your dealings with us.
The case was one well within the time scale and budget and the result exceeded our expectations. Your down to earth attitude and sense of humour made for an enjoyable experience.
I would certainly recommend your service to anyone.
Kind regards Roy and Gwen
RMJ Loudspeakers


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Convenient additional functions
Added convenience
Added convenience when cooking: with the Stop & Go function all cooking zones can be reduced to power level 1 at the press of a button, allowing you to leave the hob unattended for a short time. The Keeping warm function keeps food at serving temperature. As an extra benefit, you can set the timer so the cooking zone will switch off automatically when the time has elapsed. The Recall function ensures that your settings are saved for 10 seconds.
You can select 2 more products.
You can select 1 more product.


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Luke Proctor
Mount Horeb, WI
I work with hand forged metal. I heat metal in my forge and then shape, bend, and form it into contemporary furniture. I use traditional blacksmith techniques and joinery. I also work with mica to create the shades for my lamps. All of the shades are finished with fabric on all the edges. I use a semi-translucent acrylic for my table tops and shelves.


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Scar Removal
Laser Scar Removal Specialist in Augusta, GA
Some scars on the face and other areas of the body can cause embarrassment. At New Skin Medical, we offer non-surgical laser scar removal treatments that can significantly improve the appearance of existing scars. Effective scar removal reduces scar visibility without damaging the surrounding tissue, giving you healthier-looking skin. Dr. Sherman uses the Cynosure® Icon™ laser to minimize the appearance of scars virtually anywhere on the body.
Why Icon™ for Scar Removal?
- Approved by the FDA for the treatment of surgical scars, acne scars, and stretch marks
- Various Microlenses provide customizable treatment options for every patient's needs
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- Safely addresses scarred skin without damaging the surrounding skin
- Noticeable results can be seen after just one treatment
- Virtually no downtime afterward
- What Types of Scars Icon™ Can Treat
- Your Treatment Experience
- Surgical scars
- Acne scars
- Stretch marks
- Other signs of damaged, discolored, or aging skin
- Performed in-office for your convenience and comfort
- No anesthesia is required, but a cooling cream is applied to your skin to ensure your comfort during the procedure
- The Icon™ laser is configured with the proper Microlenses for your treatment needs
- The handheld device sends microbeam pulses of light into the skin and removes the outer layer of scarred skin cells
- The pulses of light stimulate collagen formation and the generation of new, healthy skin tissue to replace the scar tissue
- The treatment usually takes less than 30 minutes (depending on the size of the treatment area)
- You will be able to return to normal activities immediately after your procedure
- Several treatment sessions (usually between 3 and 7) may be recommended to achieve the best results
Scar Removal Frequently Asked Questions
What causes scars?
Scars form when the skin becomes injured or damaged. As the natural healing process is trigged, new skin and collagen begin to grow. Sometimes too little or too much collagen forms, disrupting the skin growth and creating a scar.
What results can I expect with laser scar removal?
The innovative Icon™ by Cynosure® laser can improve the appearance of most scars. While scars cannot be completely erased, this treatment can significantly improve the way that scars look and feel. Your treatment plan will be customized to ensure the best possible results for your needs. Using a combination of fractional laser light and the stimulation of your body's own natural healing response, the Icon™ laser will increase production of new collagen and skin cells to reduce the appearance of scars. After a few treatment sessions, your scar will become significantly smoother, lighter, and less noticeable.
When will I see my results?
Results will become visible just a few weeks after your treatment, and over the following 2-3 months, the appearance of the scar will continue to improve.
What are the side effects with laser scar removal treatment?
Some patients may experience side effects with laser scar removal. These include swelling, sensitivity, skin flaking, or a mild burning sensation. Minimizing sun exposure and using a quality moisturizer can help to protect your skin so that it heals properly after treatment.
How many treatment sessions will I need?
Dr. Sherman may recommend additional treatments beyond your first session to achieve your desired results. He will evaluate your skin type and the characteristics of the scar to determine how many treatments you need (usually between 3 and 7). Based on your customized treatment plan, each laser scar removal treatment session may be spaced 2-4 weeks apart.


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DC Comics
DC Comics Q-Master Diorama Gotham Rooftop 28 cm
Quantum Mechanix
Batman's protégés get to have amazing adventures most children can only dream of, even if they risk their lives every time they put on the cowl. But just because these kids have to grow up to do their jobs, doesn't mean they've lost the desire to play. Don't be surprised if the next time you look up in Gotham you see some small, caped silhouettes chasing...


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Bath & Body works officially debuted it's newest fragrance to the media yesterday at The Dubai Mall. Called Forever Midnight, part of the Forever Fragrance legacy, the fragrance is inspired by enduring moments in the life of today's confident, empowered woman.
Midnight is the hour of undeniable chemistry, says the perfumers. The vision of Forever Midnight took a team of three IFF perfumers, Carlos Benaim, Jean Marc Chaillan, and Clemente Gavarry, over two years to execute. A spark pf Plum nectar, Jasmine and Vanilla Orchid is what the fragrance embodies. It is exotic, velvety and has a hit of spice. A rush of Caramel adds a final sensation.
Think night blooming flowers infusing the air and that is what Forever Midnight smells like.
Bath & Body Works is located at level 1, a few meters away from Bloomingdale's at The Dubai Mall.
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BizReport : Internet : June 01, 2017
Expert: What to watch in the B2B space
2017 has been an exciting year for B2B hub CloudCraze, and several rollouts are planned for the coming months. One of the biggest changes: how B2B brands are expanding into the cloud space. We chatted with CloudCraze's Eric Marotta to find out more.
Kristina: What kind of evolution do you expect over the next 5 years?
Eric Marotta, Head of Product Marketing, CloudCraze: In the next five years, we can expect to see buyers expect more than personalization; they'll also expect businesses to anticipate their wants and needs. Technologies like AI, when integrated with commerce, allow business to learn from customer patterns and automate regular orders ‒ in addition to personalization. These experiences will manifest themselves into new touch points - call it conversational commerce. Think Alexa, Google Home, Chatbots.
But also, you can't just implement technology for technology's sake or purchase a solution based on a list of features and functions. It's about actually being able to use the technology and deploy it quickly. You want a platform and investment that allows you to experiment, learn, and then iterate ‒ allowing for continued growth overtime. The general ability to evolve is central to remaining relevant for the next five years and beyond.
Kristina: What can B2B brands expect from CloudCraze over the next year?
Eric: We started the year with a $20 million funding round, which has enabled us to supercharge product development, support continued growth in the United States and EMEA, and expand our team of B2B commerce experts. In the coming months, you can expect to see a continued focus on our customer-first commerce solution that further supports the demands of the booming B2B commerce market. We are constantly listening to customer feedback and incorporating this feedback into our platform updates. We also design with functionality and reduced time to revenue in mind. An example of this is CloudCraze Exchange, which brings the advantages of the "Apps" economy to the platform. Our first App, Mass Order, provides adjunct functionality to support Sales with taking large volume orders. Our first Connectors reduce implementation time for CyberSource and Avalara integrations from days to minutes. These are the first of many solutions planned for CloudCraze Exchange.
Kristina: Do you expect AI to continue pushing brands forward?
Eric: AI is currently making giant waves within commerce (Salesforce Einstein, IBM Watson), and we expect to see the use of this technology continue to grow. One of our customers, Kellogg's, used CloudCraze and IBM Watson to bring a custom product line to market. We are regularly looking for ways to further incorporate technologies like AI, which put the customers at the core of the commerce experience.
Tags: B2B cloud marketing, B2B trends, cloud business, CloudCraze
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text:
Premium fuel prices at petrol stations in Argentina's provinces soared as high as 43.50 pesos (US$ 1.57) per litre this weekend, after the state-run energy company YPF raised its prices for a second time in less than a week.
The 43.50-peso price tag was registered in Sauce, a city with 10,000 inhabitants located 393 kilometres south of Corrientes City, which sells YPF products.
YPF raised prices by five percent on July 1 and by an additional one percent this past Saturday, July 7.
Average premium fuel products — which car companies recommend for newer models — were selling for 36.5 pesos in Sauce at the end of June. They rose to 38.5 pesos on July 1 and 43.5 pesos (US$ 1.57) five days later.
The price hikes reflect a broader problem with pricing at fuel stations in the provinces, with the prices at Sauce reflecting a 44-percent difference with the average per-litre prices that drivers pay in Buenos Aires City. (On average, drivers in the capital pay 28.11 pesos per litre for premium.)
Competition in major cities means fuel providers are forced to keep prices low, while in the provinces some towns or smaller cities rely on just one or two petrol stations.
The Energy Ministry has tried to shift the responsibility for rising prices onto fuel providers, saying pricing "is totally liberal" in Argentina.


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The Essential Works in Political Philosophy
Original title: Les Grandes oeuvres politiques
- 2 Seas Represents: World Translation Rights.
Updated edition.
This work presents to students, teachers, and, more generally, to everyone who wishes to get acquainted with political concepts, a complete study of the foundational works of political philosophy.
By analyzing more than forty titles — antique, medieval, and, merely, modern and contemporary, from Protagoras to Foucault and Habermas but also Aristote, Machiavelli, Hobbes, Hegel — the author shows how political thought has developed around the major idea of sovereignty, which our time, especially in Europe, is about to transform radically.
This work is a extensively referenced way to undertand the political problems of our time.


text:
Valbrio, Verifysoft's UK and Ireland distributor, today announced availability of Testwell CTC++ v9.0.
Besides some optimisations, there are a number of new features including:
– Support of Objective-C
– Recognition of compile-time constant expressions
– Fingerprinting for source code files
A 30-day free trial of the Testwell CTC++ code coverage software is also available. Register for your evaluation of Testwell CTC++.


text:
- 1 Lab 1
- 2 Lab 2
- 3 Sub-heading
- 4 Reference
- 5 The Golgi Apparatus
- 6 The Mammalian Interphase Nucleus
- 7 3D Multicolor Super-Resolution Imagine Offers Improved Accuracy in Neuron Tracing
- 8 Lab 3
- 9 Article Reviews for Project
- 10 Lab 4
- 11 Anitbody against Cadherin: N-Cadherin Monoclonal Antibody
- 12 Anti-Tim23 Antibody
- 13 Lab 5
- 14 Lab6
- 15 Lab7
- 16 Lab 9
- 17 Peer Review for Group Projects
- 18 Lab 10
Lab 1
For today's attendance. --Z5011413 (talk) 15:47, 13 March 2014 (EST)
Lab 2
--Z5011413 (talk) 15:07, 20 March 2014 (EST)
This is prokaryotes.[1]
<pubmed limit=5>Prokaryote</pubmed>
- ↑ <pubmed>24595272</pubmed>
The Golgi Apparatus
AFM images of Golgi apparatus.
<pubmed>23613878</pubmed>| PLoS One.
© 2013 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Xu H, Su W, Cai M, Jiang J, Zeng X, et al. (2013) The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope. PLoS ONE 8(4): e61596. doi:10.1371/journal.pone.0061596
- Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.
The Mammalian Interphase Nucleus
The diversity in size and number of some of the major functional nuclear compartments is shown in the image.
<pubmed>17676980</pubmed>| PLoS One. Journal.
© 2007 Shiels et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Shiels C, Adams NM, Islam SA, Stephens DA, Freemont PS (2007) Quantitative Analysis of Cell Nucleus Organisation. PLoS Comput Biol 3(7): e138. doi:10.1371/journal.pcbi.0030138
- Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.
The {{The Mammalian Interphase Nucleus.}}
3D Multicolor Super-Resolution Imagine Offers Improved Accuracy in Neuron Tracing
Melike Lakadamyali, Hazen Babcock, Mark Bates, Xiaowei Zhuang, and Jeff Lichtman in their experiment used a type of super-resolution microscopy called stochastic optical reconstruction microscopy (STORM), for tracing neural connectivity using cultured hippocampal neurons obtained from wild-type neonatal rat embryos as a model system. With the advanced 3D resolution, they could distinguish and trace substantially more neuronal process in the super-resolution images rather than confocal images. The accuracy was even further improved by using a multicolor version.
<pubmed>22292051</pubmed>| [1]
©2012 Lakadamyali et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Lakadamyali M, Babcock H, Bates M, Zhuang X, Lichtman J (2012) 3D Multicolor Super-Resolution Imaging Offers Improved Accuracy in Neuron Tracing. PLoS ONE 7(1): e30826. doi:10.1371/journal.pone.0030826
Lab 3
--Z5011413 (talk) 16:30, 27 March 2014 (EST)
Article Reviews for Project
- Materials for potential import into the mitochondria are recognized by a specific receptor on the surface of the mitochondria that delivers them into a protein import channel in the outer membrane. The receptor and channel include several non-identical subunits and are referred to as the Tom Complexes ("Transport across the Outer Membrane"). The receptor consists of at least four non-identical integral membrane proteins named (after their approximate molecular masses in kDa) Tom70, Tom37, Tom22, and Tom20. This articles goes through the functions of these proteins but only Tom20 is essential for protein transport. [1]
- The two receptors, Tom22 and Tom20, are critical for the assembly of Tom40 channel units and have distinct roles as organizers of Tom40 dimers into larger TOM structures. Based on biochemical and electron microscopy studies of the TOM complex isolated from yeast mitochondria, this article discovered the molecular reason for the different number of channel-like structures. The TOM complex from wild-type yeast contains up to three stain-filled centers, while from a mutant yeast selectively lacking Tom20, the TOM complex particles contain only two channel-like structures. From mutant mitochondria lacking Tom22, native electrophoresis separates an ∼80 kDa subcomplex that consists of Tom40 only and is functional for accumulation of a precursor protein. The article concludes that while Tom40 forms the import channels, the two receptors Tom22 and Tom20 are required for the organization of Tom40 dimers into larger TOM structures. [2]
- In this article Rapaport obtained knowledge about the TOM complex through studying Saccharomyces cerevisiae and Neurospora crassa that contain homologous components to higher organisms like mammals and plants. The receptors Tom20 and Tom22 are involved in the translocation of most protein precursors, especially those with N-terminal targeting signals. Tom70, forms a binding site for a more bound set of molecules that will become proteins, most notably the mitochondrial carrier family. The subunits Tom40, Tom22, Tom7, Tom6 and Tom5 are embedded in the outer membrane where they form the Tom core complex, also called "the general insertion pore". Tom40 forms the protein-conducting channel, whereas Tom22 and Tom5 probably link the receptors to the pore (this is not concluded yet). In conclusion, the article found that Tom40 is essential for the viability of yeast and N. crassa cells. Although Tom22 was also thought to be essential, it has recently been reported that, under special genetic conditions, a very 'sick' tom22Δ strain can be isolated. None of the other Tom subunits is essential for viability in yeast or N. crass. [3]
- In the article, "Biogenesis of Porin of the Outer Mitochondrial Membrane Involves an Import Pathway via Receptors and the General Import Pore of the Tom Complex", the mitochondira from Neurospora crassa and Saccharomyces cerevisiae were used to analyze the import pathway of porin (voltage dependent ion channel) which is the most abundant protein of the mitochondrial outer membrane. The process of import and assembly of the protein is known to be dependent on the surface receptor Tom20, but the requirement for other mitochondrial proteins were explored in this article. [4]
Image Upload on Tom Complex
The TOM complex contains the initial receptors Tom20 and Tom70 (yellow). These two receptors are loosely attached to the rest of the complex (the TOM core complex); using mild detergent, they purify with the other Tom components, but not under more rigorous conditions. The TOM core complex builds the protein-conducting pore and contains Tom5, Tom6, Tom7, Tom22 and Tom40. The names of the Tom subunits reflect their molecular weights. [5]
Lab 4
--Z5011413 (talk) 15:11, 3 April 2014 (EST)
Anitbody against Cadherin: N-Cadherin Monoclonal Antibody
(class exercise)
- type of antibody- monoclonal
- species raised in- mouse
- species reacts against- chicken, monkey, rabbit, human, mouse, rat
- types of application uses- electron microscopy, flow cytometry, immunocytochemistry, immunohistochemistry, and western blot
- reference using N-Cadherin antibody- immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections and western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts
- price- 411.60 (AUD)
Reference: Sigma-Aldrich
Anti-Tim23 Antibody
- TIM23 Antibody (T-20)
- T-20 is a goat polyclonal IgG
- The working concentration for this antibody is 200 µg/ml
- Secondary antibody for IgG would be ABC-elite reagent
- This paper used the T-20 Antibody: "Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import" [1]
Reference: [2]
- ↑ <pubmed>19139266</pubmed>
Lab 5
--Z5011413 (talk) 15:16, 10 April 2014 (EST)
attendance --Z5011413 (talk) 15:08, 17 April 2014 (EST)
Exercise 1 Graph
This above experiment was specifically dealing with Tropomyosin 4.
attendance --Z5011413 (talk) 16:40, 1 May 2014 (EST)
Lab 9
attendance --Z5011413 (talk) 15:12, 15 May 2014 (EST)
Apoptosis Exercise
Hypothesis: Isolated cells grown in optimal tissue culture conditions will most likely undergo apoptosis once placed in a foreign environment.
Aim: To determine whether the cell undergoes an intrinsic or extrinsic apoptotic pathway by observing the amount of caspase 8 activated.
Method: Human adherent cells are used for the tissue culture. They are washed carefully and loose cells are isolated. Adherent cells are gently spun and labelled with FAM green fluorescence from the FLICATM kit before trypsinization. Cells are incubated with the FLICA at 37oC for roughly 60 minutes before washing. After the addition of apoptosis wash buffer, cells are placed on ice and protected from light. Observations are made by the analysis of cells with a flow cytometer. Click 'Protocol' tab in the following link (Immunochemistry Technologies) for more detailed instructions.
Suppliers: Immunochemistry Technologies [3]Resource: Green FLICA™ Caspase 8 Assay Kit
Results:If there is a high fluorescence reading observed, that means that there is a high amount of caspase 8 present and therefore, leads to an extrinsic apoptotic pathway. However, if there is a low fluorescence reading observed, there is less caspase 8 and hence, there is no extrinsic pathway activated.
Peer Review for Group Projects
Group 1
-You all are focusing on specifically phagocytosis and your topic is endocytosis. I have no idea if you have talked this over with Mark but perhaps you should briefly talk about endocytosis and the other mechanisms it includes besides phagocytosis?
-could benefit from the addition of a glossary at the end clearly defining scission, lysosome, phaolysosome, PMNs, etc.
-headings/sections could be set up in a better format
- 'Diseases' and 'Current/future Research' sections should be integrated with the rest of the content
- Maybe the overall mechanism of phagocytosis should be put before the more specific Receptors section
-The description under the 'Receptors' section gives a great overall discussion in the realm of phagocytosis. This paves the way for the sub-sections below it on specific receptor-mediated phagocytosis. This is a great idea and maybe you should add an overall discussion for your 'Mechanism of Phagocytosis' as well.
-phagocytosis is a really interesting action to see at the microscopic level so perhaps a video showing an antigen being eaten up would be cool! I think Mark showed us one during class if you are allowed to use that?
-some content seems to be referenced correctly but make sure all references are at the bottom and not dispersed throughout
-This project has a great foundation of information- the overall meat of phagocytosis is there but the organization of the content needs to be adjusted.
Group 3
-more content should be added to the blank sections obviously; when you write your intro make sure to briefly touch on the major components of your project like RanGTP/GDP, IMP and Ran-independent nuc. Import pathways, diseases, etc.
-the content cited looks very professional and clean; great job in referencing the pubmed ID for each, great sources
-why are there pictures in your references section? Is this a glitch or are you further referencing these?
-a student drawing on the basic structure on a nucleoporin would aid the discussion greatly, actually pictures of the structures listed in your project would be helpful in understanding their function
-abbreviations are a little unclear, maybe the addition of a glossary with discriptions of these would be beneficial
-I like how you used numbers to clearly point out the four different NPC Models
-the overall format of this project flows in a way that makes it easy to understand but maybe introductions to each sub-topic in the project would make it even better, for example an introduction on the nuclear pore complex before jumping into NPC Models
Group 4
-the addition of images would make the processes you are describing much more clear
-the content lacks transitional phrases and thus reads very "choppy"; try to make it flow and all connect
-the points relating to transport from neuron soma to processes are not well defined, instead of defining everything about kinesin focus more on the actual processes of transport
-a clear direction you are heading with the use of organized headings and subheadings should be added
-when you all were presenting it was pointed out that your content was moving in the wrong direction so it is difficult to give details about the body of your project but you still have a lot of time to make it work ☺ best of luck!


text:
BrandSource Readies Young Professionals University, Heavy Hitters Events
As it preps for a busy start to the summer months, independent retail buying group BrandSource announced details for its Young Professionals University event and unveiled the Heavy Hitters program planned for its Convention in Las Vegas.
The annual Young Professionals University (YPU) event, which was started in 2016, will be held in Tahoe, California June 11-14. The event is designed to bring together the next generation of industry leaders for a week of education and networking. More than 20 speakers from a wide range of areas within the industry will offer business advice to members in their 20s, 30s, and 40s.
The educational sessions at this year's YPU will cover everything from nuts-and-bolts business training, to better understanding co-workers, and finding common ground. Specific sessions include: Best and worst marketing ideas; rebranding; communication styles of men and women; how different generations work together; and strategies to build customer loyalty and drive profitability, among others.
Rob Stott is corporate communications manager, Nationwide Marketing Group


text: Currently one of the state of the art algorithms in Machine Learning is Gradient Boosting Machine (GBM). GBM can be used for regression, based on decision trees as prediction models. In GBMs, the learning procedure consecutively fits new models to provide a more accurate estimate of the response variable. The principle idea behind this algorithm is to construct the new base-learners to be maximally correlated with the negative gradient of the loss function, associated with the whole ensemble. The loss functions applied can be arbitrary, but to give a better perception, if the error function is the classic squared-error loss, the learning procedure would result in consecutive error-fitting. Read more at: http://www.dataminingblog.com/


text:
tag:blogger.com,1999:blog-1896864509547462369.post2407733833820708085..comments2020-09-10T15:43:30.956+02:00Comments on Overseas Exile: Freedom of speech is different in EuropeOvidhttp://email@example.com:blogger.com,1999:blog-1896864509547462369.post-36630287415932973682012-10-18T16:55:12.710+02:002012-10-18T16:55:12.710+02:00Can't provide a link, as it's from a subsc...Can't provide a link, as it's from a subscription legal resources website- it's current law though. The Grauniad, of course, is not just dubious in its spelling abilities, but also fond of sensationalism.

Justification (truth) has been a defence for years, but I'll just look at some of the most recent cases:

Here is an extract from Tugendhat J's judgment in LNS v Persons Unknown [2010] EWHC 119 (QB) at paragraph 96

"Before leaving the topic of defamation, I note that it is only in limited classes of cases that the law of privacy gives rise to an overlap with the law of defamation. In broad terms the cases may be considered in at least four different groups. The first group of cases, where there is no overlap, is where the information cannot be said to be defamatory (eg Douglas v Hello!, and Murray). It is the law of confidence, privacy and harassment that are likely to govern such cases. There is a second group of cases where there is an overlap, but where it is unlikely that it could be said that protection of reputation is the nub of the claim. These are cases where the information would in the past have been said to be defamatory even though it related to matters which were involuntary eg disease. There was always a difficulty in fitting such cases into defamation, but it was done because of the absence of any alternative cause of action. There is a third group of cases where there is an overlap, but no inconsistency. These are cases where the information relates to conduct which is voluntary, and alleged to be seriously unlawful, even if it is personal (eg sexual or financial). The Claimant is unlikely to succeed whether at an interim application or (if the allegation is proved) at trial, whether under the law of defamation or the law of privacy. The fourth group of cases, where it may make a difference which law governs, is where the information relates to conduct which is voluntary, discreditable, and personal (eg sexual or financial) but not unlawful (or not seriously so). In defamation, if the Defendant can prove one of the libel defences, he will not have to establish any public interest (except in the case of Reynolds privilege, where the law does require consideration of the seriousness of the allegation, including from the point of view of the Claimant). But if it is the Claimant's choice alone that determines that the only cause of action which the court may take into account is misuse of private information, then the Defendant cannot succeed unless he establishes that it comes within the public interest exception (or, perhaps, that he believes that it comes within that exception)" http://www.bailii.org/ew/cases/EWHC/QB/2010/119.html

What the Guardian article states is: "The bill includes a new statutory defence of truth which will replace the current common law defence of justification. It also includes a statutory defence of honest opinion replacing the current common law defence of fair and honest comment." - what this IMPLIES is that truth is not currently a defence. In practice, this is precisely what justification is- it is merely giving it a new name, rather than changing the substance.

The article doesn't actually say anywhere (other than by implication) that truth is not currently a defence. The changes are aimed at reducing libel tourism, but that merely means that the changes are aimed at WHO can sue in the English courts- i.e. what jurisdictions the courts have, rather than what the actual defences will be. The article does state that a new "public interest" defence is to be introduced, but that is in addition to the current firstname.lastname@example.com:blogger.com,1999:blog-1896864509547462369.post-6838671033191164222012-10-18T14:00:39.084+02:002012-10-18T14:00:39.084+02:00Hi whiskeylover, Can you provide a link for that ...Hi whiskeylover,

Can you provide a link for that and when it was passed? I refer you to this Guardian article from 2011 which discusses a bill which switches libel defenses from "justification" to "truth". I do not know if the law passed. Prior to that, the UK was famous for "libel tourism" where apparently true claims were nonetheless held to be damaging and people could be sued for all manner of silly things.Ovidhttps://email@example.com:blogger.com,1999:blog-1896864509547462369.post-4714800414566758112012-10-18T01:53:04.468+02:002012-10-18T01:53:04.468+02:00Anon 10.23. Unfortunately, the West seems to be he...Anon 10.23. Unfortunately, the West seems to be headed towards stricter control of public speech. I was appalled to read, on the Wikipedia page Dave linked for us, how many Western nations ban "insulting" people or organisations (e.g. religions). As far as I can tell, "insulting" isn't even defined; that's very oppresive.

Often, there are separate bans on what is called "Holocaust denial", which in practice seems to mean questioning specific details of the standard Holocaust report. When Jewish pressure groups ban one thing and Moslem pressure groups ban another thing, and some Christian fanatics try to ban yet another thing (Jesus portrayed as a homosexual, for instance), there isn't too much space left for us heathens!

We are very poorly served by our political representatives, aren't we, when they impose all these bans. A few generations ago, failure to attend church warranted a fine of a week's wages. I wonder if the authorities have in mind to return to that sort of compulsion. Gordon Barlowhttp://email@example.com:blogger.com,1999:blog-1896864509547462369.post-54617735371673491402012-10-17T17:04:58.032+02:002012-10-17T17:04:58.032+02:00"In the UK, unlike the US, truth is not a def..."In the UK, unlike the US, truth is not a defense in a libel case "
Really? That's news to me-

"2. DEFENCES
(1) JUSTIFICATION
82. Truth as a defence.

The defence of justification is that the words complained of were true in substance and in fact1.

Since the law presumes that every person is of good repute until the contrary is proved, it is for the defendant to plead and prove affirmatively2 that the defamatory words are true or substantially true3. If a defendant pleads justification, where the words complained of consist of statements of fact and comment, he must prove that the defamatory statements of fact are true or substantially true and that the defamatory inferences borne by the comment are true4. Truth may be pleaded as a defence to the whole of the defamatory statements or in the alternative as a defence to a severable part of them5.

1 Justification is a defence because the law will not permit a person to recover damages in respect of an injury to a character which he either does not, or ought not, to possess: McPherson v Daniel (1829) 10 B & C 263 at 272 per Littledale J. By contrast a notice of mitigation only concerns a person's actual reputation (from Halsburys Laws of England)firstname.lastname@example.com:blogger.com,1999:blog-1896864509547462369.post-87389517218665688272012-10-17T10:23:47.488+02:002012-10-17T10:23:47.488+02:00Jailing for speaking brings more problems than it ...Jailing for speaking brings more problems than it solves. Ok, slandering dead 5 year old is something else - but 4 weeks of jail for religious slander is just making martyrs and proves the "point" of radical opposition parties utilizing this rhetoric. You don't change your mind or "get correct" in 3 months of jail.

If you try to argue for more religious freedoms for minorities to counter a proposed oppression - yet a government is in fact *jailing* the people who disagree with them - who looks like the oppressed? If you are a religious minority and make public death threats for people slandering your idols - this gets protected since religious freedom and assumption of minority oppression.

Then again, people calling said religion violent and oppressive (towards women and children) get fined and jailed. This just gives ammunition to the far-right parties as the law-establishment appears to be taking a stance and jailed demagogs get martyred. They are hacking the conception of free-speech seemingly stacked against them - to promote their agenda and for proving a point.

It won't end well. On the other edge of the solution would be more strict control over public speech. Fascism. On the other end - more liberal stance towards slander and email@example.com:blogger.com,1999:blog-1896864509547462369.post-51250856947681773982012-10-17T10:21:42.287+02:002012-10-17T10:21:42.287+02:00Off you go: http://en.wikipedia.org/wiki/Freedom_...Off you go:

http://en.wikipedia.org/wiki/Freedom_of_speech_by_countryDave Hodgkinsonhttp://email@example.com


text: In light of the recent gains in marijuana legalization in the U.S., including Florida, which legalized medical marijuana in November 2016, Disney World is responding. The Florida theme park, including Magic Kingdom and Epcot, updated its park rules to add marijuana to the list of prohibited items. A Disney spokesperson said, "Although some states have legalized marijuana for medicinal or recreational use, marijuana remains illegal under federal law… We are revising our rules to clarify that marijuana is not permitted on our property." Although some marijuana advocates understand the park doesn't want marijuana smoke, they argue that many marijuana patients consume in other ways, so they're hopeful these rules may evolve.