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Why Move With ActiveTrail Cheap Marketing Automation
ActiveTrail.com doesn't require Any sort of marketing or tech expertise, as well as a first-time consumer can walk through the dash and construct messages on easy, elegant templates. ActiveTrail is a fantastic option if you're looking to start a message campaign but you're concerned your mails will go to the spam folder or simply won't get read. The predictive delivery collects data on who opened the emails and when, and helps you adjust accordingly, so that messages sent to your contacts move out in the time of day when they're most-likely to get started. Cheap Marketing Automation
click here to subscribe to ActiveTrail today.
The platform also allows you to Heavily customize the messages so that they do not come off as type letters or spam, and contains detailed reports and analytics on your messages. The autoresponder can help you schedule future messages to contacts (for birthdays and so on ), helping you keep up with the type of personal interactions which are so important for a business enterprise. ActiveTrail.com offers a free 30-day trial, therefore there is little to lose by choosing it for a test run.
Ideal for
Small and medium size businesses, that are looking for one marketing suite that includes email, SMS, automations, popups and landing pages.
Digital services that manage multiple accounts, and looking for custom attributes for their needs.
Ecommerce companies and internet stores.
Marketers that wish to automate their client journeys.
Advantages and Disadvantages
Low cost
Landing page builder
Easy to use
Responsive customer service
Somewhat restricted Selection of available templates
Many customers report slow loading times
What It Offers
Easy-to-use newsletter and email editor
Competitive pricing programs
Landing page creator
Autoresponder campaign founder
SMS campaign creator
Survey and polling tools for message recipients
Predictive delivery of messages
Detailed reports and analytics
Strong customer support
Compliant with spam regulations
More Reasons to Select ActiveTrail
No one would suggest working ad Campaigns exclusively by email these days, particularly with virtually everybody carrying out a intelligent phone with them at almost all times. The ActiveTrail SMS advertising platform brings your messaging to SMS, targeting your contacts with fast, instantaneous notes.
Together with ActiveTrail you can make Online surveys to your customers to give you feedback, helping you develop superior campaigns. It is also effective at integrating other programs in your messaging, including PayPal, WordPress and Salesforce.
The landing page builder is Optimized for mobile users and is easy for novices with no programming experience to navigate.
Setting up triggered mails is a Fantastic way to guarantee automated interaction with customers anytime they leave a message list, helping you follow-up and preserve your contact base.
ActiveTrail's sign-up types are Easy to make and can be integrated in multiple manners in your newsletters and email. Just like virtually everything else on ActiveTrail, the types are highly customizable.
Ease of Use
The ActiveTrail interface is Easy-to-use and ought to be somewhat familiar to anybody who has used a simple website builder. It enables you to easily export your contacts or enter them manually, and walks you through every procedure. When you hit "make an email" you get 5 choices — routine email (either with a template or your own HTML code), A/B split email (make two versions and see which works best), on event email (an email to be delivered to the individual automatically on particular dates — such as a birthday), triggered email (one that is sent when a person unsubscribes or subscribes), and RSS email (an email which will be sent automatically based on an RSS feed).
After clicking on your selection, a template loads with an easily customizable box of alternatives. Here you are able to upload images and make a gallery on your account that you readily pull pictures from in the future. Cheap Marketing Automation
click here to sign up for ActiveTrail today.
The shipping options are easy to Arrange according to contact lists which you can upgrade at will. This permits you to make multiple variations of a targeted effort for multiple lists of individuals with different traits and interests, which you can track through ActiveTrail's analytics program.
The same ease of use applies to Creating landing pages and SMS campaigns.
ActiveTrail currently offers 30 Templates for newsletters and 25 for landing pages. While that's a rather sizable collection, there isn't much selection, especially compared to site builder websites which provide hundreds, even thousands of potential templates.
Nonetheless, there are a fair Variety of choices.
The platform allows you to save Your templates for later repeat usage. The templates include blank or quite threadbare ones to more vibrant and dynamic presentations. You will find templates suited for makeup companies looking to highlight deals, car dealers, holiday-themed Christmas templates, food and dining-themed templates, and people for families and holiday-goers.
The offerings seem to rely Marginally heavily on stock photos and easy, even generic designs, but each alternative is extremely customizable.
Customer Support
Helpful customer service is a Powerful feature of ActiveTrail–and no small thing when it comes to your company's promotions. Queries delivered to ActiveTrail's customer support representatives were answered efficiently. A customer support question sent via the website's contact form at 9pm on a Saturday night (CST) was replied 4 hours while an email sent to the customer service email at the same time was answered 4 hours afterwards.
Irrespective of the Subject at hand, The client service answers were kind, helpful, and asked for clarifications so that they are of more help.
That said, in comparison to other technology Sites, ActiveTrail's customer service might leave something to be desired. There is not any live chat window or 24/7, 1-800 hotline to contact in the heat of the moment.
ActiveTrail's predictive delivery Is an effective instrument for discovering when and how each and every contact reads emails, so you aren't just carpet-bombing your contact list and hoping for the best. You are able to test out what works with a first campaign or two and then use the machine to customize the messages for your customers.
Their autoresponder and occasion Mails take the brainwork from keeping up with clients' birthdays, anniversaries, vacations and so on. The triggering emails also give customers a way to reach out to clients if/when they decide to go out.
ActiveTrail's competitive pricing Strategies are well-suited equally to small boutique businesses that has a few of clients to contact, and large corporations who can scale to massive plans. The user has the leeway to choose which program works best for them.
The landing page founder is a Fantastic way to create a special promotional site for one-off events or sales, and with the website's poll and polling tools, message recipients have the capacity to weigh in about what works and doesn't–and then integrate that data in future attempts.
Together with ActiveTrail's survey solutions you can learn a Whole Lot about your End-users and their demographics, finally taking a few of the guesswork out and giving you the confidence to serve their needs better. Cheap Marketing Automation
Designing and publishing a survey is an easy and straightforward process. Pick the query types and fill in the particulars. ActiveTrail takes care of everything else. You can integrate your polls in to your automation workflows.
Pick between open-ended, single, multi-select and personal info questions. In addition, select personal info, a matrix of selection and Net Promoter Score (NPS) that provides you an overall indicator of consumer satisfaction.
ActiveTrail sets itself apart with simple powerful editing programs. Begin by picking from one of those well-designed pre-made templates categorized by context and purpose, such as fresh technology product, restaurant promotion or vacation advertisement.
At this stage, half of this job has been performed for you. All you Have to do is edit the text and graphics to suit your organization.
Users can add pre-defined'blocks' that are essentially micro-templates Such as blocks of text, pictures, sign-up types, and even widgets and buttons for Facebook and Paypal. Embedding these micro-templates is only a case of dragging and dropping them in your own creations. Save cubes as a template for later usage.
ActiveTrail has an exhaustive list of integrations that greatly enhances The usefulness of the service. Here are a Few of the most common integrations:
· Twitter — Add an ActiveTrail registration form to your site, which adds users to the ActiveTrail recipient list, ultimately increasing the size and efficacy of your email marketing efforts.
· SalesForce — Synchronize your client list, giving you easy access to a Salesforce contacts within ActiveTrail.
· Google Analytics — Ascertain data which will otherwise be difficult to find, like the number of contacts opening your mails, their demographics or the percentage of contacts creating a buy.
· Facebook Lead Ads — Mail contacts which have finished a Facebook registration type. Additionally, incorporate social networking cubes to your landing pages.
Other integrations include Cashcow, Optimove, Personalics, Excel and most CRM platforms.
SMS campaigns
With ActiveTrail, you can send text messages to contacts, providing you Another route for communication subscribers. This is particularly significant with the ubiquity of mobile phone usage.
Contacting users via text is a cheap and simple process, and much like Setting up an email advertising campaign. There's the added functionality to program the when you want to send the SMS campaign, ensuring it reaches your clients at the most optimal time.
Marketing Automation
ActiveTrail Provides a substantial number of solutions for promotion Automation, making sure you engage with clients on an individual level, while also freeing yourself from repetitive actions.
ActiveTrail includes nine templates to account for the most common Activates you might want to send an email for, such as a contact unsubscribing, a touch celebrating their birthday, a contact or clicking onto a hyperlink.
Each template is fully customizable, and you can create your personal Automation entirely from scratch. Cheap Marketing Automation
Every automation process has a start and end, but in between, select As many or as few actions as required. You can do just about anything; for example, make an automation to send subscribers an email in their birthdays, then after a timed delay of your choice, yet another email could be sent that reminds contacts to get products they have in their basket.
Advanced Features
Several innovative functions Makes your life easier when importing data.
One of the very useful is the'Update existing contacts' checkbox that Mitigates the risk of creating duplicate contacts, but it readily enables you to update existing contact information without needing to find and edit individual contacts.
Another Superb resource for touch import direction is that the import Background section, which gives you an overview of all imported contacts. From here, determine how many contacts were inserted, updated, deleted, and even how many errors there were throughout the importation process.
Errors rows and wrong formatting are flagged up providing you the Opportunity to rapidly fix the issue.
Advantages of ActiveTrail
Straightforward interface
The interface is straightforward and clutter free, which makes navigation A breeze. The dash features four big widgets for emailcontacts, landing pages and reports, all of which are obviously segmented.
The editing interface is remarkably user-friendly as it consists of an Instinctive visual pipeline that designed with brevity in your mind.
You're never more than 2-3 clicks away from adding a second step to your Automation procedure, and you never need to trawl through pages of choices looking for the appropriate action, making it appropriate for all those new to marketing automation.
Everything about the interface is logically placed and organized, Ultimately meaning you create complex and dynamic automations without any training.
The free-form interactive whiteboard allows you to drag-and-drop actions Onto at your leisure. The non-restrictive layout enables you to organize your automation in such a manner so that you know it.
Bunch of useful templates
The service comes with email templates for newsletters and emails which Are easily customisable with drag and drop functionality.
This gives you the opportunity to make professional looking email, Newsletter and SMS campaigns in only a couple of clicks.
No technical knowledge required
Users do not need technical knowledge of HTML, CSS or graphic design in Order to utilize ActiveTrail, even though it's always an advantage, as always.
But even the least tech-savvy user can use the applications readily, Selecting you won't need to hire extra staff or spend time training your existing workforce.
ActiveTrail offers a transparent pricing tier where each single Functionality is available across each and every tier. Users don't have to worry about not accessing key attributes for choosing a cheaper price program.
The pricing is based on the Amount of contacts the business wants to Contact, beginning at $500 for up to 500 contacts. Cheap Marketing Automation
The email and SMS bundles have their own independent pricing models. All these are one off payments where consumers get a fixed quantity of emails or SMS to send to get a set price, which is beneficial for businesses seeking to do a single email or SMS burst. For emails, the starting price point is $25 for 1000 mails. For SMS, the starting price point is $41 for for 500 SMS'.
ActiveTrail is a full-featured,versatile cloud-based email marketing Service excellent for small, medium and massive businesses.
This software solution knocks down the hurdles inherent with advertising. Create professional-looking email and SMS campaigns, cheaply, with only a couple of clicks. Additionally, make use of advanced solutions, such as surveys, landing pages, A/B testing and analytics.
Overall, ActiveTrail helps boost psychological bonds with present and Prospective clients, which is very important to company seeking to construct their customer base and expand the reach of their own brand. With low prices and high usability, ActiveTrail is a fantastic choice for marketing campaigns.
Click here to subscribe to ActiveTrail today.
click here to subscribe to ActiveTrail today.


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Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh.
Received date: 07/02/2015 Accepted date: 22/03/2015 Published date: 26/03/2015
Visit for more related articles at Research & Reviews: Journal of Microbiology and Biotechnology
Cell signaling and signal transduction are very important for a cell so that it can govern basic and advanced cellular activities in a well-coordinated manner. The activities of cells include anabolic and catabolic activities, response to their microenvironment and external environment, response to various ligands either beneficial for cell or harmful for cell and other physiological activities (sight, touch, taste, pain, anxiety, anger etc). Transduction involves the binding of extracellular signaling molecules and ligands to cell-surface receptors that trigger events inside the cell. Among the various types of receptors studied GPCRs are considered to be of utmost importance. The members of this large family of proteins are activated by a spectrum of structurally diverse ligands, and have been shown to modulate the activity of different signaling pathways in a ligand specific manner. They play a key role in regulating cell activity and physiological function. GPCR malfunction is responsible for a wide range of diseases including nephrogenic diabetes insipidus, and hyperthyroidism and a large proportion of drugs on the market target these receptors. The knowledge of GPCRs structure and function is very important for elucidating the molecular mechanisms underlying effective signal transduction and diseases and for performing structure-based drug design. Since structural data are restricted to only a handful of GPCRs, a major population of researchers is engaged in deducing the structures of various GPCRs. The main aim of this text is to introduce the fundamentals of GPCRs with emphasis on structure and function of rhodopsin, a well studied GPCR belonging to the rhodopsin family of GPCR and responsible for vision in vertebrates.
cell signaling, signal transduction, GPCR, autocrine, ligand
The capacity of cells to communicate with one another to administer fundamental cell activities and coordinates cell activities is known as cell signaling. Biological processes require various cells to work together in a well-coordinated manner. To make this possible, cells communicate with each other, by cell signaling. Cell signaling makes it possible for cells to respond in an appropriate manner to a specific environmental stimulus.
Types of cell signals
1. Autocrine signaling- cell that is giving the signal by producing the messenger molecule also expresses receptors on its surface that can respond to that messenger. The start and end of the signal is the same cell.
2. Paracrine signaling- messenger molecules travel only short distances through the extracellular space to cells that are in close proximity to the cell that is generating the message. Paracrine messenger molecules travel only short distances because they are unstable and can be degraded by enzymes.
3. Endocrine signaling- messenger molecules reach their target cells via passage through the bloodstream. The start of the signal is basically a gland and the end of the signal is a distantly located cell.
4. Juxtacrine signaling(contact-dependent signaling)- Two adjacent cells must make physical contact in order to communicate. This requirement for direct contact allows for very precise control of cell differentiation during embryonic development.
Signal transduction occurs when an extracellular signaling molecule activates a cell surface receptor and this receptor then alters the intracellular molecules creating a response. The signal can also be amplified depending on the need of the tissue, organ or body. There are two stages in this process-
1. A signaling molecule activates a specific receptor protein on the cell membrane.
2. A second messenger transmits the signal into the cell, producing a physiological response.
Cell signaling is initiated with the release of a messenger molecule/ligand by a cell that sends messages to other cells in the body. Other cells can only respond to an extracellular message if they express receptors that specifically recognize and bind that particular messenger molecule [1]. The messenger molecule/ligand binds to a receptor at the extracellular surface of the responding cell. This interaction causes a signal to be relayed across the membrane to the receptor's cytoplasmic domain [2]. A route taken to elicit a particular response depends on the type of receptor that is activated. There are 2 major routes of signal transduction-
1. Receptor transmits a signal from its cytoplasmic domain to a nearby enzyme, which generates a second messenger. Because it brings about the cellular response by generating a second messenger, the enzyme responsible is referred to as an effector [3]. Second messengers are small substances that typically activate (or inactivate) specific proteins. Depending on its chemical structure, a second messenger may diffuse through the cytosol or remain embedded in the lipid bilayer of a membrane [4].
2. Another type of receptor transmits a signal by transforming its cytoplasmic domain into a recruiting station for cellular signaling proteins. Proteins interact with one another, or with components of a cellular membrane, by means of specific types of interaction domains [5].
• These receptors are included in every physiological movement, including sight, taste, and hormone regulation [6]. They work as sign transmitters that can sense an immense assortment of signs and increase them inside the cells, prompting diverse cell reactions, and are included in numerous human diseases.
• Among the signals transduced are light (phototransduction), extracellular calcium ions, tastants (gustatory), odorants (smell), pheromones (mating signals), warnings (pain), immunological (chemokines), endocrine (hormones), and neural (neuromodulators and neurotransmitters) [7]. They are the richest source of targets for the pharmaceutical industry as ligands directed at GPCRs represent the largest family of pharmacological agents, accounting for nearly 30% of current clinical pharmaceutical agents available [8].
• Our understanding of GPCR structure is based largely on the high-resolution structures of the inactive state of the human β2 adrenergic receptor (β2AR), the avian β1AR, the human A2A adenosine receptor and rhodopsin [9]. Rhodopsin is better suited for structural studies than most other GPCRs because it is possible to obtain large quantities of highly enriched protein from bovine retina. Rhodopsin is a remarkably stable GPCR, retaining function under conditions that denature many other GPCRs [10] .
• Great progress has been made over the past three decades in understanding diverse GPCRs, from pharmacology to functional characterization in vivo [11]. Cryoelectron microscopy, X ray crystallography and other advanced techniques have provided insights into the molecular mechanisms of GPCR structure, activation and function [12].
Basic Structure of GPCR
G protein coupled receptors (GPCRs) represent the largest family of membrane proteins or cell- surface receptors in the human genome (approx 2%). All GPCRs are located within the plasma membrane [13] . Research using Co-immunoprecipitation and resonance energy-transfer techniques has successfully demonstrated that GPCR structures are present at the plasma membrane [14].
GPCRs are cell- surface receptors capable of coupling to specific guanine binding proteins (G proteins), thereby transducing an extracellular signal to an intracellular effector. As a family of proteins, GPCRs share a common structural signature of seven hydrophobic transmembrane (TM) α-helical segments, with an extracellular amino terminus and an intracellular carboxyl terminus [15]. The helices are connected to one another by three extracellular loops E1, E2, E3 and three cytoplasmic loops C1, C2, C3. GPCRs share the greatest homology within the TM segments [16]. The variable structures among the family of GPCRs are the carboxyl terminus, the intracellular loop spanning TM5 and TM6, and the amino terminus. The greatest diversity is observed in the amino terminus. This sequence is relatively short (10–50 amino acids) for monoamine and peptide receptors, and much larger (350–600 amino acids) for glycoprotein hormone receptors, and the glutamate family receptors [17]. The largest amino terminal domains are observed in the adhesion family receptors. The extracellular loop E2 and the N-terminus segment are especially well situated for ligand binding. These structures vary in size from one class of GPCR to another [18]. The intracellular loops plus the cytoplasmic ends of the trans membrane helices participate in protein binding and activation. The cytoplasmic tail and third intracellular loop (C3), in particular, provide multiple sites for protein docking and interactions [19,20].
Examples of GPCRs with well-ascribed roles in cardiovascular biology include the β1- and β2- adrenergic receptors (ARs), the α1- and α2- ARs, the M2- and M3-muscarinic acetylcholine receptors, the angiotensin II (Ang II) receptors, the endothelin receptors, the adenosine receptor, the thrombin receptor, and the vasopressin receptor [21–25].
A recent and detailed analysis of the human genome reveals over 800 unique GPCRs (Yona, 2010). Based on sequence similarity within the 7 TM segments, these receptors in vertebrates can be clustered into 5 families-
1. The rhodopsin family (family A, 701 members)
2. The secretin family (family B, 15 members)
3. The glutamate family (family C, 15 members)
4. The adhesion family (24 members)
5. The frizzled/ taste family (24 members) .
The physiologic function of a large fraction of these 800 GPCRs is unknown; these receptors are referred to as orphan GPCRs [26].
The rhodopsin family is the largest and most diverse, and members are characterized by conserved sequence motifs that imply shared structural features and activation mechanisms. Receptors for a diverse spectrum of ligands including most of the amide and peptide hormones and neuromodulators belong to this family [27–29]. Class A receptors bind a number of glycoprotein hormones such as LH, TSH, and MSH using a large extracellular domain. The glycoprotein-binding receptors differ from the GPCRs for the smaller ligands. The latter receptors rely on extracellular loops or utilize a binding pocket formed by the H3 to H6 helices [30].
Ligands for Class B GPCRs, such as glucagon, secretin, and VIP/PACAP (vasoactive intestinal peptide/pituitary adenylate cyclases activating polypeptide) are fairly large. Receptors in this family utilize an extracellular domain plus nearby extracellular loops, for ligand binding [31–33].
There are no orphans among the Secretin family and the Frizzled family, but two thirds of the glutamate remain as orphans. Only seven of glutamate has been identified as drug targets. An extensive extracellular domain and a large intracellular domain characterize this group [34,35].
The adhesion family has a much extended N terminus thought to be involved in cell-cell contact. There are, as yet, no drugs targeting them, and the majority of them remain orphan receptors [36]. But knockout studies in Caenorhabditis elegans and in rodents showed that they are crucial in the early development of the embryo and also appear to have a number of physiological roles [37].
Synthesis and Maturation
Both during and after the synthesis, GPCRs undergo a continual process of maturation before reaching at the plasma membrane. They achieve proper folding while residing in the endoplasmic reticulum, traverse from the cis- to the trans- Golgi while undergoing modification, and finally targeted to the plasma membrane where they are properly inserted into the membrane [38–40].
Strict quality-control systems inside of cells guarantee that despicably or deficiently collapsed proteins are focused for degradation, ordinarily by means of the proteasome pathway [41]. Folding of many GPCRs into a proper/functional conformation requires the presence of endogenous accessory chaperone proteins. For example, the human DnaJ protein HSJ1b, a member of the heat shock protein (HSP) family of cytoplasmic cochaperones, regulates trafficking of rhodopsin from the endoplasmic reticulum (ER) to the cell surface. Alternatively, single-membrane–spanning chaperone proteins can facilitate GPCR exit from the ER [42–45].
Oligomerization of GPCRs plays in the biosynthesis and trafficking of nascent GPCRs to the cellular surface. Multiple GPCRs including the β2-AR and vasopressin receptors undergo constitutive homodimerization early in the biosynthetic pathway, occurring in the ER. Early studies using rhodopsin, muscarinic, and β-adrenergic receptors as model GPCRs suggested that GPCRs exist primarily as monomers, although modification of the detergent extraction systems used for protein purification also suggest that a varying fraction of GPCRs are present in oligomeric form. In order for a GPCR to transduce an extracellular signal, it must both traffic correctly to and be retained at the cellular surface to allow for receptor/ligand interaction. Multiple proteins not directly involved in the signal transduction cascade have been identified which stabilize receptor surface expression. These include spinophilin, Homer, actin-binding protein 280/filamin A, protein 4.1N, muskelin, and postsynaptic density-95 (PSD-95) [46-48].
The effect of a ligand on the structure and biophysical properties of a receptor, and hence on the biological response, is known as the ligand efficacy [49]. Natural and synthetic ligands can be grouped into different efficacy classes as (a) full agonists are capable of maximal receptor stimulation(b)partial agonists are unable to elicit full activity even at saturating concentrations (c)neutral antagonists have no effect on signaling activity but can prevent other ligands from binding to the receptor(d)Inverse agonists reduce the level of basal or constitutive activity below that of the unliganded receptor [50].
Ligands act as either agonists or as antagonists. An agonist induces the same response in the receptor as that triggered by the natural ligand. An antagonist binds to the receptor but the receptor does not transmit a signal in response to the binding event [51]. By binding the receptor the antagonist blocks access of the natural ligand to the receptor and thus prevents transmission of a signal. Drugs that bind in an antagonistic fashion are known as blockers [52]. Prominent examples are beta blockers, which antagonize the beta-adrenergic receptor, and antihistamines, which inhibit the histamine H1 receptor.
The ligands range from subatomic particles (a photon), to ions (H+ and Ca++), odorants, fatty acids, neurotransmitters, amino acids, peptides, proteins and even proteolytic enzymes . The location of the ligand binding domains for many GPCRs has been determined [53]. While many small organic agonists bind within the TM segments, peptide hormones and proteins often bind to the amino terminus and extracellular sequences joining the TM domains.
Heterotrimeric G Proteins and Subunits
GPCRs transmit messages into the cell by activating heterotrimeric G proteins. In the absence of GPCR ligand binding, heterotrimeric G proteins remain in close association with the GPCRs. Ligand binding leads to G protein dissociation and signaling through second messengers that target protein kinases/phosphatases and ion channels. Heterotrimeric G proteins are assembled from three distinct subunits. These subunits are designated as Gα, Gβ, and Gγ. There are 20 different Gα subunits, 6 known Gβ subunits and 12 distinct Gγ subunits.
The Gα subunits function as GTPases. The signaling activity of a Gα subunit is turned off when it is GDP-bound and switched on when it is GTP-bound. The GPCR provides the activation signal and also serves as the GEF (guanine nucleotide exchange factor), catalyzing the dissociation of bound GDP from Gα. A family of proteins called regulators of G protein signaling (RGS proteins) function as GAPs (GTPase Activating Protein) for the Gα subunits. They catalyze the hydrolysis of GTP by the Gα subunits, thereby rapidly switching off Gα signaling [54].
Once activated the Gα and Gβγ subunits are free to diffuse laterally along the cytoplasmic surface of the plasma membrane, and bind nearby signaling targets, or effectors. The cycle of activation and signaling is completed with hydrolysis and reassociation of the Gα and Gβγ subunits. Upon binding Gα, the Gβγ induces substantial conformational changes and increases the affinity of Gα for GDP. When bound to Gα, the Gβγ subunit cannot bind its effectors and signal because the binding sites on Gβγ for its effectors overlap that for Gα.
Families of Gα subunits
There are four kinds of Gα subunits. For most GPCRs one type of GPCR couples to and activates only one of the four kinds of alpha subunits. However, in some cases, the coupling is richer and the GPCR can switch from one kind of subunit to another [55].
Several portions of the GPCR contribute to the G protein-specific binding. Regions at the ends of helices H3, H5, and H6, loops C2 and especially C3, and the short helix (H8) located in the C-terminal region just after helix H7 are all involved in coupling and activating the various members of the heterotrimeric G protein family.
1. Gs subunits- bind to and stimulate adenylyl cyclases
2. Gi subunits- inhibit adenylyl cyclases
3. Gq subunits- stimulate phospholipase C
4. G12 subunits- effectors are not identified yet
Each Gα family contains several variants. Some variants and groups of variants are found in certain tissues while others are more broadly distributed. For example- G0 subunits are brain-specific.
Gβγ subunits target many of the same second messenger generators as the Gα subunits. Like Gα subunits, some Gβγ subunits stimulate adenylylcyclases while others inhibit them. Still other Gβγ subunits stimulate phosopholipase C. Thus, Gα and Gβγ subunits jointly determine the overall action of a G protein on second messenger generators [56] .
Structure of Rhodopsin: A G Protein-Coupled Receptor
Rhodopsin, also known as visual purple or visual pigment, is a biological pigment in photoreceptor cells of the retina that is responsible for the first events in the perception of light. Rhodopsins belong to the G-protein-coupled receptor family and are extremely sensitive to light, enabling vision in low-light conditions.
Rhodopsins are a member of the largest GPCR family i.e. the rhodopsin family. These are activated by light and turn on the signaling pathway that leads to vision. Rhodopsin is an integral membrane protein with about 50% of its mass in the phospholipid bilayer [57].
Rhodopsin is composed of the protein opsin (Bovine opsin consists of 348 amino acids ([9]), 40 kD) covalently linked via a protonated positively charged Schiff base linkage to 11-cis-retinal through Lys- 296. Glu-113 serves as the counterion for the Schiff base attraction of the chromophore to Lys-296. Lys residue is conserved within H7 in all pigments, and Glu residue that serves as the counterion to the Schiff base is conserved within H3.
The 11-cis-retinal chromophore is a derivative of vitamin A1, with a total of 20 carbon atoms. The binding site of the chromophore lies within the membrane-embedded domain of the receptor. All seven transmembrane segments and part of the extracellular domain contribute interactions with the bound chromophore. The chromophore is located closer to the extracellular side of the transmembrane domain of the receptor than to the cytoplasmic side. In all, at least 16 amino acid residues are within 4.5 Å of the chromophore: Glu-113, Ala- 117, Thr-118, Gly-121, Glu-122, Glu-181, Ser-186, Tyr-191, Met-207, His-211, Phe-212, Phe-261, Trp-265, Tyr-268, Ala-269, and Ala-292.
Two Dimensional Crystals
The first structure of rhodopsin came from cryoelectron microsopy and X- ray crystallography of bovine rhodopsin from Gebhard Schertler's group. While the resolution of these structures was limited (ranging from 5 to 9Å), they provided the first picture of the orientation of the TM segments in a lipid environment.
The seven alpha helices in rhodopsin are arranged sequentially in a clockwise manner when viewed from the intracellular side. Helices 1, 2, 3, and 6 are tilted. That is, their axes are inclined by about 25° relative to an axis drawn perpendicular to the surface. Helices 4 and 7 are nearly perpendicular to the membrane bilayer. Helix 6 is bent; one part is nearly perpendicular to the plane of the membrane and the other part is inclined by about 25° (helix 5 is also bent, but to a lesser extent) [58]. Palmitoyl acyl chains, covalently bound to Cys 322 and Cys 323, are thought to anchor part of the carboxyl terminal tail to the membrane forming a putative fourth loop consisting of 11 amino acids on the cytoplasmic surface of the rod outer segment disk membrane. An additional posttranslational covalent modification is found in the amino terminal tail at Asn 2 and Asn 15, where N-linked oligosaccharides are added.
Three Dimensional Crystals
Three dimensional crystals structures of rhodopsin have been obtained by several groups. Three-dimensional crystals of bovine rhodopsin have been grown using at least two different approaches.
The first published crystals of rhodopsin were obtained from rhodopsin selectively solubilized from rod outer segments using a combination of alkyl (thio) glucoside detergents and divalent cations. The procedure used no additional purification steps. The mixture likely contained more rod out segment lipids than would be expected from protein purified by column chromatography, and it was speculated that the presence of these lipids may influence crystal formation [60]. Crystals grown from this preparation of rhodopsin initially diffracted at 2.8 Å and subsequent improvements have led to diffraction at 2.2 Å. All of these crystals have a P41 space group, and crystal contacts form between hydrophilic domains of the receptor.
Three-dimensional crystals have also been obtained from bovine rhodopsin solubilized from rod outer segments using the detergent lauryldimethylamine-oxide (LDAO) and subjected to lectin chromatography followed by detergent exchange into n-octyltetraoxyethylene (C8E4) followed by anion exchange chromatography. This more extensive purification procedure, which would be expected to remove all but tightly bound lipid, resulted in crystals diffracting at 2.6 Å. These crystals have a P31 space group and crystal contacts form primarily within the transmembrane domains and the intracellular and extracellular loop structures point into solvent filled cavities [61]. So the loop structures may assume a more native structure in the P31 crystal form compared to the P41 crystals.
Comparison of P41 and P31 rhodopsin structures
The structures obtained from P41 and P31 crystals are very similar overall, particularly in the transmembrane and extracellular domains. However, there are significant differences in the cytoplasmic loop linking TM5 and TM6, which is known to be involved in G protein coupling. The structure from the P31 crystals is in agreement with structures obtained from electron diffraction of two-dimensional crystals and with electron paramagnetic resonance spectroscopy studies. As discussed above, this loop may assume a more native structure in the P31 crystals because none of the cytoplasmic domains are involved in crystal lattice contacts [62].
Function of Rhodopsin
The rhodopsin molecule operates in a switchlike manner to activate the G protein. Two of the helices, H3 and H6, project into the cytoplasm further than the others. Absorption of a photon by the 11-cis-retinal causes its isomerization to all-trans-retinal, leading to a conformational change of the protein moiety H3 and H6, including the cytoplasmic surface (martin, 9). The photolyzed chromophore only transiently activates opsin, before the all trans- retinal is hydrolyzed and dissociated from the opsin [63].
When activated by ligand binding the GPCR acts as a guanine nucleotide exchange factor, or GEF, for its G protein (transducin, Gt). In its active shifted conformation, the rhodopsin GPCR is able to catalyze the release of GDP from the alpha subunit of the G protein leading to its binding GTP. The GDP-GTP exchange triggers the dissociation of Gαβγ into Gα and Gβγ subunits and the subunits migration towards their effectors. The switch is reset by the dissociation of the ligand from the GPCR. The absorption of a single photon results in the activation of hundreds of G-protein molecules with extraordinary reproducibility, whereas the 11-cis-retinal–bound rhodopsin has extremely low activity [64].
The α subunit of Gt, Gtα, activates the effector enzyme, a cGMP phosphodiesterase (PDE), by binding its inhibitor subunit, thus initiating cGMP hydrolysis. The reduction in cGMP concentration results in a hyperpolarization of the ROS plasma membrane, due to the reduced flux of Na+ ions through the cGMP-gated channels, with concomitant change in neurotransmitter release at the synaptic end of the rod cell, which generates the neuronal response to light [65].
Figure 8: Model depicting the activation of the GPCR rhodopsin based on recent X-ray crystallographic structures. On the left, rhodopsin is shown in its inactive (dark-adapted) conformation together with an unbound heterotrimeric G protein (called transducin). When the retinal cofactor (shown in red on the left rhodopsin molecule) absorbs a photon, it undergoes an isomerization reaction (from a cis to a trans form), which leads to the disruption of an ionic linkage between residues on the third and sixth transmembrane helix of the protein. This event in turn leads to a change in conformation of the protein, including the outward movement of the sixth transmembrane helix (red curved arrow), which exposes a binding site for the Gα subunit of the G protein. The rhodopsin molecule on the right is shown in the proposed active conformation with a portion of the Gα subunit (in red) bound to the receptor's cytoplasmic face.
Figure 9: Visual signal transduction in rod cell. Light (hv) converts rhodopsin (R) to an activated form (R*), which binds and activates Gt(GDP) by catalyzing the exchange of bound GDP for GTP. Gt(GTP)* then dissociates and Gtα(GTP) binds to the the hydrolysis of cGMP by PDE* αβ. The lowered concentration of cGMP induces closure of cGMP-gated Na+ channels in the plasma membrane, hyperpolarizing the cell.inactive form of the cGMP-phosphodiesterase (PDEi). This complex dissociates to yield the active subunit complex PDE* αβ and Gtα (GTP)*. PDEγ , where PDEγ is the inhibitory subunit of the PDE, initiating
Much of vertebrate physiology is based on GPCR signal transduction. GPCRs convey the majority (80%) of signal transduction across cell membranes . As the receptors for hormones, neurotransmitters, ions, photons and other stimuli, GPCRs are among the essential modes of communication between the internal and external environments of cells. Since GPCRs mediate most of our physiological responses to hormones, neurotransmitters and environmental stimulants they are the major therapeutic targets for a broad spectrum of diseases.
Although all GPCRs are characterized by the presence of seven membranespanning α-helical segments separated by alternating intracellular and extracellular loop regions but individual GPCRs have unique combinations of signal-transduction activities involving different types of ligands and multiple G-protein subtypes and complex regulatory processes [66].
The wide spectrum of ligand efficacies for individual GPCRs shows that efficient energy transfer between the binding pocket and the site of G-protein interaction is dependent on multiple interactions between receptor and hormone and requires more than simply occupying the binding site. Many GPCRs can stimulate multiple signaling systems, and specific ligands can have different relative efficacies to different pathways [67].
Taking the human β2AR as an example, the binding of adrenaline and noradrenaline to cells in the target tissues leads to the activation of the stimulatory subunit of the heterotrimeric G protein (Gαs), the stimulation of adenylyl cyclase, the accumulation of cyclic AMP (cAMP), the activation of cAMP-dependent protein kinase A (PKA) and the phosphorylation of proteins involved in muscle-cell contraction. For example, β2AR exhibits significant constitutive activity, which can be blocked by inverse agonists. The β2AR couples to both Gαs and the inhibitory subunit (Gαi) in cardiac myocytes, and can also signal through MAP kinase pathways in a G-protein-independent manner through arrestin. Similarly, the process of GPCR desensitization involves multiple pathways, including receptor phosphorylation events, arrestin-mediated internalization into endosomes, receptor recycling and lysosomal degradation. These activities are further complicated by factors such as GPCR oligomerization, localization to specific membrane compartments and resulting differences in lipid-bilayer composition. Such multifaceted functional behavior has been observed for many different GPCRs [68].
There has been remarkable progress in the field of GPCR biology during the past two decades. Notable milestones include the cloning of the first GPCR genes, and the sequencing of the human genome revealing the size of the GPCR family and the number of orphan GPCRs. Moreover, there is a growing appreciation that GPCR regulation and signaling is much more complex than originally described, and includes signaling through G protein independent pathways.
Ligand-induced selective signaling (LiSS)
The LiSS concept was introduced by Terry Kenakin and is rapidly becoming a generic theme for GPCRs. It has important implications in specific drug development and in minimizing side effects. As we are clear by now that different ligands selectively recruit different intracellular signaling proteins to produce different phenotypic effects in cells. How GPCRs operate is conventionally viewed as the receptor occurring in two states: an inactive state and an active state. In the active state the receptor induces and activates a cascade of events culminating in the physiological function of the cell [69].
What has recently emerged is that receptors can assume many conformations. Each of these conformations can potentially interact with a ligand in a highly selective manner. In turn, this specific receptor conformation interacts with a specific intracellular signaling complex. Nowadays it is becoming popular that the nature of the ligand and the dynamically changing intracellular environment alter the level and path of signaling. Now screening for novel ligands will not simply involve receptor binding but will screen for the appropriate intracellular signal, which reflects the desired phenotypic response of a cell for a disease state or pathophysiology [70].
GPCR signaling independent of G proteins
Another area of research is the GPCR signaling through proteins other than G proteins. It is becoming increasingly apparent that there are many ways in which GPCRs can signal independently of G proteins. The first convincing evidence for the existence of GPCR-independent signaling was given by Lefkowitz and associates. Angiotensin II binds to AT1 receptor and activates both β-arrestin and G proteins [71,72]. When blockers like losartan and valsartan (antagonist) blocks the AT1 receptor, no intracellular signal was propagated. However, another type of antagonist (SII) does not activate the G protein pathway but exclusively recruits β -arrestin and activates ERK.
GPCRs are a large family of cell surface receptors that respond to a variety of external signals. Binding of a signaling molecule to a GPCR results in G protein activation, which in turn triggers the production of any number of second messengers. Through this sequence of events, GPCRs help regulate an incredible range of bodily functions, from sensation to growth to hormone responses.

subset: commoncrawl


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This minimal October birthstone cuff is composed of 18k gold plated sterling silver and a light rose SWAROVSKI® crystal. Pair with our October Light Rose Birthstone Hook Earrings and gold -toned jewelry.
Pre-order: Please allow 6-8 weeks for delivery.
18k gold plated sterling silver, light rose SWAROVSKI® crystal
Approx. 5.5" in circumference


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The Game is getting set to release his 5th studio album Jesus Piece. Last week we got a look at the controversial artwork, and now he has taken to his Twitter to reveal the release date of the highly anticipated project.
[blackbirdpie url="https://twitter.com/thegame/statuses/262058193671311360″]
Looks like Jesus Piece will be hitting store shelves December 11th and will have features from Kanye West, Nicki Minaj, DJ Premier, Trey Songz, Chris Brown, Scarface, Lil Wayne, Wiz Khalifa, Tyga and much more.


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Easter Sugar Sandwich Cookies
Make these fruit-filled sugar cookies from our Test Kitchen throughout the year by using different cookie cutter shapes.
Total TimePrep: 10 min. + chilling Bake: 10 min. + cooling
Makes1-1/2 dozen sandwich cookies and 1-1/2 dozen small cutouts
- 1/2 cup shortening
- 1 cup sugar
- 2 eggs
- 1 teaspoon lemon extract
- 2-1/2 cups all-purpose flour
- 1/2 teaspoon salt
- 1/4 teaspoon baking soda
- 1/3 cup raspberry filling
- 1/3 cup apricot filling
- In a large bowl, cream shortening and sugar until light and fluffy. Beat in eggs and extract. Combine the flour, salt and baking soda; gradually add to creamed mixture and mix well. Cover and refrigerate for 1 hour or until easy to handle.
- Divide dough in half. On a lightly floured surface, roll out one portion to 1/8-in. thickness. Cut with a floured 2-1/2-in. round cookie cutter. Repeat with remaining dough. Using a small floured bunny- or chick-shaped cookie cutter, cut out the center of half of the cookies.
- Place cookies and cutouts 1 in. apart on ungreased baking sheets. Bake at 350° for 8-10 minutes or until edges are very lightly browned. Remove to wire racks to cool.
- Spread bottoms of the solid cookies with filling; place cookies with cutout centers over filling. Serve with cutouts.
Nutrition Facts1 each: 188 calories, 6g fat (2g saturated fat), 24mg cholesterol, 98mg sodium, 30g carbohydrate (14g sugars, 1g fiber), 2g protein.
Every product is independently selected by our editors. If you buy something through our links, we may earn an affiliate commission.


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Thank you for being a loyal and supportive patron of South Suburban Parks and Recreation. We appreciate your patience and understanding during our closures due to COVID-19.
South Suburban wants you to play it safe! Several of our
facilities are opening in accordance with the Safer at
Home guidelines. Learn more at
Let's celebrate you! Choose how to enjoy your Day with specially-themed birthday packages at locations across South Suburban. Planning your celebration with us is a piece of cake!
Our indoor turf facility is open for your birthday parties large or small. Invite the whole class or the whole team. Time to ENJOY!
Family Sports Dome
Party Packages
$175 Resident, $200 Nonresident
To Book a Party
Please call the Sports Dome at 303.754.0541 or email email@example.com. A party is considered booked when full payment is received and you receive back a confirmation request. Space is limited to a first come, first serve basis.
Add creativity to any child's birthday with one of our art parties.
Parties can held at these locations Buck, Goodson, Lone Tree, Sheridan (Creativity Lab) recreation centers or The Lone Tree Hub.
Party package
Two (2) hours of party time, including an hour-long lesson from a professional instructor.
$175 Resident, $190 Nonresident
Other information
Ages 4 and up, each child under 4 must be accompanied by one adult
Up to 15 participants maximum
To Book Your Party
Please download our Art Party Request Form first.
Email your completed form and request for a party to email@example.com. You will receive a call from Monique for payment information. Your party is considered booked when full payment is received and you receive back a confirmation request.
For more information, contact Monique Keohane, birthday party coordinator, at 303.708.3513.
Youth tennis players, new and experienced, have a fun tennis birthday party.
Indoors at the Littleton Tennis Bubble or a South Suburban outdoor tennis court.
$175 Base price
Additional food available from Centennial Grill's food and drink menus. See costs associated with extra food and drink.
To Book a Party
Please contact email@example.com.
Riders, get ready to race.
County Line BMX
Party Packages
• April – October, 8 am – 4 pm (some times unavailable due to prior commitments)
• Party has sole use of the BMX track
• Rentals must be at least two weeks before the party
• Weather determines if the track ready for your rental
• Signed permit and payment required before party to secure requested time
• Shelter, picnic tables, bleachers available
• The host may bring pop-up tents, decorations, food and drinks (no barbequing or glassware allowed)
Party Package Pricing
$40 Resident, $50 Nonresident, rental per hour
Additional fees
$30 for the use of the gate - Mon – Fri 9 am – 4 pm
To Book a Party
Contact Jeremy Kopischke, Track Director at firstname.lastname@example.com or 303.483.7028.
South Suburban Parks and Recreation6631 S University BlvdCentennial, CO 80121


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Areas of Expertise: State wildlife policy, aquatic and riparian conservation, wildlife corridors, Upper Rio Grande region and human dimensions of carnivore conservation
As Southwest Representative for New Mexico, Michael focuses on wildlife habitat connectivity, Mexican gray wolf policy, protecting and recovering aquatic and riparian ecosystems and species, state wildlife policy and renewable energy siting and transmission. Most recently, Michael served as Defenders' New Mexico Outreach Representative for Government Relations for four years. While in that role, he worked across the state with partner organizations and media outlets to increase public support and awareness for wildlife conservation and to mobilize citizen advocates.
Before joining Defenders, Michael worked for the Montana Wilderness Association as their communications and campaign fellow and as the field director for North Dakotans for Clean Water, Wildlife and Parks gathering signatures for a ballot measure in North Dakota to dedicate existing oil tax revenue to conservation. He also worked as a tour guide driving guests throughout Grand Canyon and in Yellowstone National Parks.
Michael earned a master's degree in environmental history from the University of Montana where he began work on his book, Grizzly West, which focuses on the attempt to reintroduce grizzly bears to the Selway-Bitterroot Wilderness in Montana and Idaho. He received his B.A. from Brandeis University.


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Now showing items 1-1 of 1
Study on SMV and line balancing of garments
(Daffodil International University, 2018-04-06)
We completed our thesis on "Study on SMV and Line balancing of Garments". This paper clearly explains the total operation of long sleeve shirt and SMV calculation. Total SMV of long sleeve shirt is 22.41 and we also done ...


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Meyer, 'Foolish' wisely play to win
There's a car commercial in which Mr. Sandman accidentally douses a sleeping young man with an extra big dose of his magic dream dust.
Suddenly, the young man's wildest dreams converge all at once: he's speeding around a track in a cool car while his favorite rock band serenades him and hundreds of women in bikinis cheer him on.
Then there's Brad Meyer, a senior at Stevenson.
He hasn't needed any magic dream dust.
And yet, he's leading a life every single day that many teenage boys would consider a Mr. Sandman-like dream.
Meyer made two varsity athletic teams at Stevenson: football and wrestling. He's been on each team for the last two years, as a lineman and a heavyweight, respectively. Meanwhile, in his spare time, he's the lead singer and drummer for a popular band on campus that not only has its own groupies, but has played numerous gigs around the suburbs and is about to have all of its songs cataloged on iTunes.
Sports and music. Is there much more to life for teenage boys?
"I have had an absolute blast in high school," Meyer said. "To be able to do so many different things and to have so many different kinds of opportunities has been so cool."
The 6-foot-2, 275-pound Meyer got involved in sports "because I've always been big," and he grew to love the experience, especially when he got to be part of special moments like Stevenson's historic football playoff victory over perennial heavyweight and nemesis Maine South last fall.
But even that high couldn't compare to the way Meyer feels when he's jamming with fellow Stevenson seniors Jordan Gates, Donald Bender and Kai Funahashi. They are his buddies from "Foolish," the band that Meyer helped to create when he was in sixth grade.
"For me, music always trumps sports. I love it so much," Meyer said. "When I was younger, I kind of had ADD. I could never sit still, so my parents wanted to see if they could get me involved in something that could keep me focused. We decided on drum lessons. They thought that would be good for me, and it really was.
"It really worked. It was a way to focus me, and it also gave me a way to vent. It really kind of tempered the ADD. But as I kept going with it, it developed into so much more."
Music led Meyer down roads he could have never dreamed of, even with Mr. Sandman's magic dust.
As a freshman, Meyer led "Foolish" to an unexpected win in the Buffalo Grove Park District's "Battle of the Bands," a contest that involved at least eight other bands from the area. "Foolish" was the youngest band in the mix.
"The ages for all the people in the bands were like 14 to 20, but most of the bands were in their 20s," Meyer said. "We wrote a handful of original songs and when the judges announced that we won, they said that we were more original than the other bands. They liked that we had a drummer as the lead singer. They liked that one of our guys plays saxophone. They thought our sounds were original."
"Foolish" is a rock band with jazzy undertones. All of the band members have been a part of Stevenson's jazz band.
"I had to give up jazz band once I started making varsity teams at Stevenson because sports started taking up a lot of time for me," Meyer said. "But before that, when I was younger, I was always in jazz band and that's where I met the guys (from "Foolish"). We all started jamming and everything just clicked."
While Meyer sings and plays drums, Gates plays bass, Bender plays guitar and sax and Funahashi plays the piano. Gates and Bender have played alongside Meyer since freshman year while Funahashi joined the group this year.
"It's been a really good experience," Gates said. "We've had a lot of fun together, writing songs, playing shows. About a year ago, we sat down and talked about where we wanted to be (at the end of senior year) and this was it."
"Foolish" wanted to be getting regular gigs, which it has. And it wanted to have enough original songs to release an EP (a mini-album) that could be listed on iTunes. Check mark on that, too.
"Being in this band has taught me so much about different things: working with others to achieve a common goal, working through tough times for the long-term," Bender said. "We're doing now what we've talked about doing for a long time and that feels really good."
"Foolish" has played around 35 gigs in the last couple years and has come out with four songs: "It's Alright," "Reinvent the Wheel," "Troubles," and "Rewind." The songs, which can all be heard on the band's website: http://foolish-music.com, are part of the EP "The Last Laugh."
For "Foolish," whose members will all be going to different colleges next year, the EP represents their first triumph together, and quite possibly their "last," as in "Last Laugh."
"Even though I'm a newbie in this band, I've had so much fun and I'm going to miss everyone next year," said Funahashi, who will be going to Oklahoma State while Meyer will be going to Columbia in Chicago and Gates and Bender will be going to Wisconsin and Purdue, respectively. "We have done things with this band that I would have never thought I would be doing. I've realized that because of this opportunity, music is something I'll be doing for the rest of my life, now."
And maybe as a part of "Foolish."
The group hasn't ruled out reuniting during breaks from college or even after college.
"Our ultimate goal has been to go out with a blast and that's why we're putting our music on iTunes, because we want to make as many people familiar with our music as possible before we go off to college," Meyer said. "But I could see us getting back together. Hey, if people start liking our music and we get enough followers on iTunes, you never know what might happen."


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Safe Routes to School
UP Signs Lease for New Parking Lot
Posted on April 25, 2013
The parking lot on Union Pacific Railroad property at the end of F Street is expected to be open for use by Memorial Day weekend, May 25. City staff received the signed lease April 15 from Union Pacific (UP) officials. Approximately 60 parking spaces will be available, including four to five over-sized lots. Planned improvements […]
Filed under Administration, Community Development, News, Safe Routes to School
BikeFest – Celebrating Bikes of All Kinds!
Posted on August 17, 2012
The City of Salida will host the first ever Salida BikeFest from September 12 – 16. BikeFest has several goals, which include promoting safe bicycling, getting more people on bikes, helping local bicycling/trail organizations with awareness and fundraising, and of course just having fun in Salida celebrating bikes! Many different organizations in Salida, including the […]
Filed under Featured, News, Recreation, Safe Routes to School


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'Girls': Superficial Changes and Free Snacks
This week on Girls, Hannah quit her job at Ray's to take a gig at GQ magazine, writing about stereotypical urban men as part of an advertorial campaign. And, amazingly, "Free Snacks" wasn't just about Hannah. Here's what's going on which all of the girls (and Ray and Adam).
Hannah's luck is actually looking up this week, but it's not long before she sees it as being quite the opposite. The episode opens on Hannah at Ray's, telling Ray that she's quitting her job at the coffee shop (again). Ray is appropriately flippant about her announcement, and says he'll see her again next week when whatever bullshit gig she's quitting for falls through. It turns out that Hannah's writing got her noticed by GQ, but Ray rightly points out that she wouldn't have been recruited to be an actual GQ writer, and that she's probably been commissioned to write advertorial content (the paid articles that are really just ads in disguise) and is therefore both morally and creatively bankrupt. It hurts because it's true.
When Hannah gets to GQ, she's immediately won over by the perks, mostly the fact that the mag's kitchen is stocked with free snacks to which she oh-so-freely helps herself (full disclosure: I considered applying to work at GQ during this scene).
Hannah pretty much owns her job at GQ. She's great at stereotyping and being snarky, so her ideas are a big hit. Unfortunately, it puts her on bad terms with one of her fellow advertorial writers and leads to a discussion about how she's a "real" writer and assumes they are not. When she learns that they all have roughly the same aspirations and credentials as she does, she freaks out and half-quits the job, before taking it back and vowing to dedicate her nights and weekends to writing. That works about as well as it does for anyone who makes that vow (she ends up asleep on her couch moments after arriving home).
Shoshanna comes back into the forefront this week. She has roughly her fifth quarter-life crisis and decides that her wild child phase is over and she's choosing to unchoose her bad choices. She decides that the best way to do this is to get into a committed relationship, STAT. She picks the cutest and dumbest of her hookups for the honor. It's cringe-worthy in what an obviously bad decision it is.
Marnie continues to be abhorrently annoying, but Ray seems to be into it, presumably because she's hot? (She's not remotely nice to him, so it can't be that.) They go on an awkward date and he pretends to give a damn about her life. It's also bad news bears in the making. But when are the decisions on Girls not?
Jessa is working at the children's store she applied to last week and convincing naive social climber moms that black, too-small-for-their-children dresses are good for Christenings. It's funny in a throwaway kind of way, but tells us nothing meaningful about Jessa or where she is in life. Considering her escape from rehab in the season opener, it would be nice to followup on her a little more.
Ray has always been my favorite character on the show, but I'm super disappointed by his decision to pursue Marnie. She's still in a doom-bound spiral and treating him like total shit. He deserves better and seeing him try to be the guy she wants (especially when she clearly doesn't know what she wants) is just depressing.
Adam got a callback for an acting audition. He's really excited about it and then Hannah falls asleep during his celebration and he's respectful and sweet and covers her with a blanket. So, basically, Adam is still in his own world and he and Hannah still have a pretty unbalanced relationship.
Overall, even though a lot seemed to happen to the characters this week and a lot of changes were happening in theory, everyone is very much standing still. Sorry, girls.
Image: Getty Images


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[3/29/17] WSDOT Has One More Meeting Tonight, In Manson For Details On Upcoming Projects
Public invited to learn more about three projects on the north and south shores of Lake Chelan | WSDOT
CHELAN – Drivers who use State Route 150 or SR 971 near Lake Chelan are invited to learn more about several highway construction projects that may affect them starting this spring.
Washington State Department of Transportation staff will hold a pair of open houses on March 28-29 to answer questions about a new roundabout and paving work on SR 150 and a slope stabilization project above SR 971.
SR 150 and No See-Um Road intersection improvement
- Roundabout construction begins in April.
- Estimated project completion in 2018.
SR 150 Manson to Chelan paving
- Paving work scheduled to begin April 10.
- Estimated project completion in July 2017.
SR 971 Slope stabilization
- Rockslide on South Lake Shore Drive occurred on March 8.
- Construction expected to begin in late spring.
The open houses will provide community members with an opportunity to see plans for the projects, tentative work schedules and possible traffic impacts.
Open house information
Wednesday, March 29
Where: Manson Parks and Recreation meeting room, 142 Pedoi St., Manson
When: 4 to 6 p.m.
These are drop-in open houses with no formal presentation, where people can visit and ask questions of staff anytime during the two hours.


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2 Speak unto the children of Israel, saying, If a woman have conceived seed, and born a man child: then she shall be unclean seven days; according to the days of the separation for her infirmity shall she be unclean.
3 And in the eighth day the flesh of his foreskin shall be circumcised.
4 And she shall then continue in the blood of her purifying three and thirty days; she shall touch no hallowed thing, nor come into the sanctuary, until the days of her purifying be fulfilled. (Lev. 12:2-4)
If we look at these verses only through the lens of equality of the sexes we may think it unfair to pronounce a woman unclean for bearing a child. But if we look at it as a type of Christ, we may appreciate it better.
Christ is the means by which we are to be "born again," so the newborn represents the born-again soul. Just as the innocent Christ takes the sins of the soul that is born again, the innocent mother submits to be considered ceremonially unclean for a certain period.
Though we don't know how much pain Christ went through to deliver us all from sin, the Lord must have thought that associating it with childbirth and delivery pains would help women understand it better. I think this shows His concern for faithful women and it suggests that He really does know what women go through.
Continue reading at the original source →


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We've seen some strong earnings and bulls regained some control in the market. Apple (AAPL) will post their earnings November 1st, and this might actually move the market. So far, price of Apple, jumped within few days after earnings
Google (GOOGL) will post earnings today, I have a little concern about that, so I am not looking for a trade there.
Facebook (FB) will most likely go down after earnings, it's been on a down trend, and it broke support several times since last earnings report. Current uptrend could be a bull-trap, so be careful.
Nvidia (NVDA) is on a downfall and will likely fall through earnings report as well, next month.
Adobe (ADBE) is at support, and can go either way, if it breaks support at 240 then it will go down further. However, ADBE in on an uptrend and will regain it's momentum back up soon. Not trading it.
Netflix (NFLX) is a no trade in my opinion, volatility, and indecision makes it hard to trade, stock trend changed.
Boeing (BA) is neutral, so possibly a good position trade. Not buying the stock though.
AMD (AMD) might be a great opportunity to buy, but wait for the confirmation, at least 3 days to see where it moves.
Amazon (AMZN) looks way down, so no trade for me, although the earnings will tell where it will probably go later.
Caterpillar (CAT) is on a downtrend, might be ok to buy and hold, but I don't think it found support yet.
Costco (COST) could be on uptrend after dropping after earnings, and then regaining some momentum. Might a good trade.
Dropbox (DBX is going the way Snapchat (SNAP) went it seems. No trade there, perhaps a bearish trade. I think it's best to invest my money elsewhere.
EBAY (EBAY) is bearish.
Lowes (LOW) and Home depot (HD) are on a downtrend and will probably stay there. Stocks are at the bottom, so might be ok to buy and hold now in anticipation of a next uptrend.
Microsoft (MSFT) will most likely go up, I will probably trade it in few days, perhaps monday.
Micron (MU) I am not touching it now, it's down and will probably take some time to regain momentum up. Until then, it's on a downtrend.
NOC, LMT, RTN are down, and not a trade for me, although they could be ok in the long term.
What I am not touching; the marijuana stocks. No way I am getting in right now, I feel sorry for all people who bought TLRY at 300. The stock is at 110 now and will most likely go down, even after earnings. Even if it has good earnings report, I am expecting a selloff right after the report next month.
ETFs and Indexes; Too early to tell, will wait few more days, although the buy back seems really nice.
Gold is going down, again. It looks like selloff, so will wait few more days to make sure. Not trading it for now.


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|Topic: Are any of these prophecies fulfilled by Jesus?
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If you want to discuss Jar, then please start a new thread.
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No, I have never seen a single supportable example of prophecy of Jesus, particularly none from the Old Testament.
Let's look at Mark in context.
I in the version of the story found in Mark 14, the author has the character Jesus talk about his premonitions of a coming death. If the stories about Jesus are true, that should have been obvious to him as well as many others. He was telling folk that it was time to stop just accepting what they were told and to start questioning the popular dogma; and that is never a popular position.
Regardless, the particular scripture is not referenced in the passage but it is most likely referring to the earlier passage in Mark 14 where he asks to be spared. It is the acceptance that death is the inevitable result of his behavior.
Here is the story in context.
Note also, this story evolves much as all urban legends evolve over time as it gets retold in the other synoptic Gospels.
It also might help if instead of you saying what it is I believe, that you ask ME what it is I believe, but that should be in another thread, it is irrelevant to this one.
Anyone so limited that they can only spell a word one way is severely handicapped!
Member (Idle past 3433 days)
Posts: 360
From: Phoenix Arizona USA
Here we go with gospel according to jar again. The text does not say any of this. Again what the scripture clearly states in Mark 14:49 is ..."THIS HAS TAKEN PLACE TO FULFILL THE SCRIPTURES"!
The Old Testament were the only scriptures there were at that time.
Think about what you are saying jar. What happened earlier wasn't scripture yet.
I was repeating your profession made on the testimony thread. I could look it up for you but as stated it is for another thread.
Posts: 32930
From: Texas!!
Member Rating: 2.1
Which, if it were true, is a classic sign of false and created prophecy; which by the way, it most likely was. I included the full passage to let folk see how the author of Mark told the story and, since it does not specify what scripture is involved, either refers back to earlier in the conversation or is a false and created prophetic fulfillment just as with your example I cover in Message 19 of Zacharia and Matthew.
I also doubt from what you post that you have any more idea what the word scripture means than the word prophecy.
Scripture simply means an inspired writing. It does NOT mean Canonized writings. In fact, at the time Jesus lived it is unlikely anything except perhaps the first five books had been Canonized.
According to the stories there were lots of "scripture" floating about and in common use that never made it into many of the Canons. A good example is the Book of Enoch, and that is one that it seems Jesus himself was familiar with or at least the writers give the character Jesus lines that show that he referred to it.
The point is, based on that text just as with every other example you have presented, I see no indication of either prophecy or fulfilled prophecy.
If you can show support, great, please do so.
Anyone so limited that they can only spell a word one way is severely handicapped!
Member (Idle past 3433 days)
Posts: 360
From: Phoenix Arizona USA
Mark was quoting what Jesus said. Mark was written from A.D. 50 to 70. Its obvious Mark wasn't referring to what Mark had written but what Jesus had said at the time before his crucifixion.
It is plain to see Jesus was referring to what was about to happen. Nowhere in scripture does it ever say Jesus had a premonition either. He always spoke with authority. Never did he speak the words: "I think; maybe, might or could.
Again in Mark 14:49 He spoke with absolute authority that what was taking place WAS fulfilling
To call it a premonition proves my point that you are a false teacher and a deceiver as I have stated all along.
.....and what ever your next posts are my answer is "your wrong"
end of story
Member (Idle past 2348 days)
Posts: 2688
From: UK
Edited by Panda, : fomatting
Posts: 32930
From: Texas!!
Member Rating: 2.1
The author of Mark was writing what he wanted the character Jesus in the story to say, perhaps even what he thought he remembered Jesus saying, but since the story evolves with each telling and the details change and since it was written at least a quarter century after Jesus died, it is highly unlikely to be what Jesus actually said.
It is also clear that since it does not specify what scripture did get fulfilled that it cannot be considered fulfilled prophecy. I can with equal authority claim I too fulfill scripture and if I was really pressed, I'm sure I could do a better job than the author of Mark did in supporting my position.
A claim to have fulfilled scripture is NOT prophecy and unless the exact scripture is mentioned it is in fact meaningless.
It also appears that in addition to scripture and prophecy, you do not understand what the word premonition means.
I included the section from Mark in case you or some readers had not read it and I imagine they can see that in the story, Jesus goes and asks, prays, that what is about to happen doesn't. That implies that he at least hoped that what certainly seemed inevitable could be avoided.
That too is priceless.
Thank you.
Anyone so limited that they can only spell a word one way is severely handicapped!
Posts: 9069
From: god's waiting room
yes, it does clearly state that. however, we have the benefit of having said scriptures, with which we can compare such a statement.
if i told you yesterday "i'll give you back that five dollars tomorrow", and today i gave you fifty cents and said "obligation fulfilled!" you'd look at me a little funny and demand your $4.50. just the fact that i said i have fulfilled something doesn't mean that i have.
i understand that the bible gets a special pass with you, and most fundamentalist christians, but there's really no good reason why this should be the case. it is a simple logical disconnect -- $0.50 never equals $5.00. when you have the contract to compare the payment to, it's relatively easy to check. in this case, the "fulfilled" prophecy is zechariah 13. let's check it.
since i know you didn't read that just now, i'll give you the cliffs notes.
because numbers 1 through 5 of those things actually happened in jewish history. josiah, king of judah and from the house of david, did in fact purify jerusalem, removed idols, and kill false prophets. after josiah's death, jehoahaz briefly reigns and then is succeeded by jehoiakim. babylon invades. jehoiakim is replaced by jeconiah, who is cursed by the prophet jeremiah. judah is carried off into babylonian exile, with about a 1/3rd of the population remaining under babylonian rule.
considering that zechariah is writing at the time of the babylonian exile, it's quite silly to think he's talking about something else that just happens to be almost identical to the major political situation in his world. zechariah is primarily concerned with re-establishing the temple in jerusalem, and purifying judaism, and when persia -- darius the great -- takes over, he is one of the people that helps establish second temple judaism.
but let's look at the shepherd again.
note the rest of the verse which has obviously been omitted in the "fulfilled prophecy". if we are to suppose that the shepherd is christ (and not the cursed king jeconiah, and not the coming messiah as in jewish tradition), where is the part where god smites the christians? surely, i have missed that part of the gospel. oh, and lest you think the "wounded hands" bit above is another random christ reference, remember that zechariah is talking about false prophets.
in closing this chapter just fits its historical context too well to even be prophecy (in the sense that you mean) in the first place. most of the part christ is claiming as fulfilled prophecy (by him) in mark had already been "fulfilled" well before zechariah even wrote. the only bit that zechariah is actually predicting is the return from exile, and the re-establishment of the jewish religion.
this is a bit like me saying, "i'll give you back that five dollars tomorrow", giving you back five dollars the next day, and then 600 years later also giving you a nickel so that my obligation has been fulfilled.
it's worth noting that the old testament likely wasn't fully canonized during the time of christ. there was quite a lot of debate during that period about what should go in the third section of jewish scripture.
christ refers repeatedly to "the law (torah) and the prophets (nevi'im)" which are two sections of jewish scripture. the law (torah) is the five "books of moses" and the prophets (nevi'im) are joshua, samuel/kings, isaiah, jeremiah, ezekiel, and the 12 minor prophets (including zechariah). the other books, like job, psalms, esther, ruth, etc, were likely not part of the official canon yet. jesus does refer to the psalms, however.
it's also worth noting that there is a wealth of extra-canonical literature that simply never made the cut.
Edited by arachnophilia, : No reason given.
Posts: 32930
From: Texas!!
Member Rating: 2.1
Just a bump to see if anyone thinks that there is an Old Testament prophecy that even refers to Jesus.
Anyone so limited that they can only spell a word one way is severely handicapped!
Posts: 32930
From: Texas!!
Member Rating: 2.1
So he can show us all the support for a unity of theme and pupose between the Old and New Testaments that refers to Jesus.
Just a note Dawn, let's take your claimed support one example at a time; the actual chapter and verse please.
Anyone so limited that they can only spell a word one way is severely handicapped!
Posts: 9069
From: god's waiting room
i have officially given up trying to direct dawn to this thread. he wants to make claims regarding prophecy, but does not wish to participate in this thread where his claims and our arguments would be on-topic. at the same time, he wants to pretend like we don't have an argument, because we try to keep discussions in their appropriate tactics. this is a particularly devious debating technique, and one i suspect nearly everyone can see straight through.
however, in an effort to actually explain our argument in his thread, slightly off-topic though it may be, i have contributed a rather lengthy hand-holding reading comprehension analysis of isaiah 7 to his thread. it is complete with historical context showing how it was already fulfilled around the time it was given, and a demonstration regarding how it really cannot have anything to do with jesus.
people may wish to hold a more enlightened discussion on the topic here, in the appropriate thread. i imagine this will totally derail dawn's thread, as it has dome many a prophecy thread. as if dawn's thread was really ever on any rails to begin with, right?
Posts: 9069
From: god's waiting room
okay, so thanks to iTanakh, i have found a particularly interesting doctoral thesis on the topic of isaiah 7:14.
it's about 300 pages, so i haven't by any means read all of it yet. but i've skimmed through some important sections of the first two chapters, and it largely confirms what i've been saying. it does, however, hint at an interesting take on matthew's reading of isaiah 7:14, but does not seem to elaborate.
it quotes some scholarship (ch2, II, 1) that tries to affirm the nonsense that dawn has been spouting -- he doesn't get this stuff from nowhere. not scholarship, of course, but scholarship filtered through clergy, filtered through fundy pastors. of course, it goes on to rebut those claims in much the same way i have: i devalues the prophecy and turns it into nonsense, and prophets were not fortune-tellers.
in any case, the text argues, on the whole, that imanuel was king hezekiah. this in part due to the context of the two assyrian invasions, the structure of proto-isaiah, and some of the textual details. for instance, it affirms my less than standard analysis that הָרָה "is pregnant" is essentially present tense, while קָרָאת שְׁמוֹ "will call his name" is essentially future tense. (biblical hebrew lacks actual time-related tenses, but it's not too hard to figure out, and generally works much the same way as modern hebrew) the author also points to the fact that the LXX translations "you will call his name" and not "she will call his name", indicating that the LXX translators understood that king ahaz was the father -- imanuel was his son.
i would quote the pertinent sections, but copying is disabled. i don't particularly feel like finding a work-around, or re-typing it all myself. and if the author has copy-protected it, it's likely because he wants people to read it in its source.
Member (Idle past 1927 days)
Posts: 5069
From: Zerus
I normally don't participate in prophecy threads because it's simply not within my interest. I just wanted to quickly pointed out something that I've been talking about for years.
Scientific minded people, or at least those who are skeptics, police their own ranks. If I were to come out and say "all christians are murderers and rapists", no doubt among my biggest critics will be my fellow skeptics.
On the other hand, let me quote ICDesign in this thread.
This is pretty much "lalala I can't hear you" dressed in biblical quotes. Through the 7 pages of this thread, I have only seen skeptics criticism of ICDesign's behavior. IDists and Creationists, where art thou?
By staying silent, the sensible IDists and creationists seem to encourage the more extreme, crackpot elements within their ranks to prosper.
I'm sure I'm not the only one who have made this observation over the years.
Member (Idle past 2009 days)
Posts: 301
From: Burlington, Canada
All this is incredibly dumb.
The NT was obviously tweaked to make it look like ancient prophecies were being fulfilled. What else would you expect in religious propaganda?
Suspended Junior Member (Idle past 3271 days)
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This is my first time i visit here. I found so many entertaining stuff in your blog, especially its discussion. From the tons of comments on your articles, I guess I am not the only one having all the leisure here! Keep up the good work.
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Our baby boy is finally here and he is perfect in every way! I went into labor Saturday evening and I was not expecting it! I remember waking up Saturday morning and Dakota and were joking about how that could have been our last night sleeping as just the two of us – little did we know it was true. That morning I got up to go shopping with my mom and we walked around for hours just looking at furniture and other shops. I lost my mucous plug that afternoon and was freaking out because I knew that meant we were making progress! Typically women don't go into labor until 3-5 days after losing their plug so I didn't think much of it other than we were one step closer.
That night around 9 pm Dakota and I were watching TV and it happened – a small gush that was definitely not normal! I got up to go to the bathroom and could tell that my water had totally broke! I couldn't believe it. So naturally I walked back to the living room and casually said, "Babe I think we should really go to the hospital now – I'm pretty sure my water broke…" HA! When we got to the hospital it was after hours so we had to call the nurses station to let us in. I wasn't sure what to say so when they answered I said, "Yes hi. I think I'm having a baby." I know – I'm so awkward. They took us back into a room where they checked to see how far I had progressed and I was dilated to a 3 but my water had definitely broke so they admitted us.
I got an epidural right away and it was heavenly! I wasn't having too bad of contractions yet but I wanted to be ready in case I progressed really fast (and the nurses suggested to get it sooner rather than later) and it was so nice. At 3 am I was dilated to a 5 and by 4 am I was at a 9 – I couldn't believe it! They called my Doctor and she was there within 15 minutes and we started pushing. It was the most surreal and difficult 40 minutes of my life. I knew that I was about to have my baby in my arms but everything was going so fast I couldn't process it all. Plus pushing when you can't feel you legs or core is so weird! I felt like I was just making an intense face the whole time rather than actually pushing haha but I guess I really was because at 5:14 am we had a beautiful baby boy in our arms!
I heard his cry right away and was instantly relieved and felt so much joy and love for him already. They put him on my stomach and cleaned him off a bit and Dakota cut the umbilical cord before they took him to be weighed and measured. They brought him back to me for skin to skin and it was overwhelmingly beautiful. Then seeing Dakota hold him just melted my heart! He is so perfect and we are so blessed to have our beautiful healthy baby boy finally with us.


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Inside everyone's wardrobe, there are a few key items: the go-to's, the study hoodie, the going-out tops, the little black dress LBD and of course, the favorite. Steinhardt sophomore Iman Dabbous' favorite blazer envelopes her in the warmth of her Notting Hill home back in London. Dabbous bought the pink, vintage Comme Des Garçons blazer while out on a shopping excursion with a close friend.
"I love and miss Notting Hill so much, the place, in itself, has so much sentimental value to me." Dabbous said. "It's where all of the important things in my life happened, so this jacket kind of signifies that for me."
The artistry of the jacket adds to the appeal for Dabbous. Comme Des Garçons is a brand well-known for the heart logo displayed on its T-shirts and hoodies, as well as for its partnership with Converse, a popular footwear choice around NYU. The classic Comme Des Garçons branding isn't emblazoned across her jacket rather it's an understated pink –– Dabbous' favorite color.
"I love it because it's so unique. I've never seen anything like it from this brand, I was so shocked when I saw the label." Dabbous said. "It kind of looks like the Chanel blazers but in a color Chanel would never use."
Beneath the branding, Comme Des Garçons is also known for its craftsmanship. The brand was the theme of the annual Met Gala in 2017 for its avant garde designs and silhouettes. Dabbous was shocked by how her piece fit perfectly.
"Everything was in proportion and that's something that can be hard to find in vintage," she said. "A lot of the times things are just either too big or too small."
Despite Dabbous' description of the perfection of her jacket, she makes it clear that this is not a part of her everyday wardrobe.
"I love it so much that I am a little scared to wear it," she said. "I tend to save it for special occasions. I wore it to a party here once, but that just meant I had to stay away from people with full drinks in their hands, so now I just wear it when the opportunity arises."
Despite the limited closet space that most NYU dorms or New York apartments provide, there is always room for that one piece that means more than meets the eye, even if it spends more time in your closet than on you.
Email Hanna McNeila at [email protected]


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The Confident DIY Painter
Would you rather be a do-it-yourselfer? The very first step is downloading the book The Confident DIY Painter. In it I'll reveal the 6 Things Every DIY Painter Needs To Know, along with the Most Common Mistakes They Make and How To Avoid Them!
10 Secrets Every DIY Cabinet Painter Needs To Know
Have you ever wondered how do professionals get cabinets exactly the same every single time? Well, I'm ready to let you in on the secret… with the 10 Secrets Every DIY Cabinet Painter Needs To Know
5 Questions To Ask Before Choosing A Cabinet Painting Contractor
When searching for a cabinet-painting service for your kitchen or bathroom, you'll probably want to get two or three quotes from different contractors to determine the best fit for you.
We've compiled a few essential questions you should ask contractors, including us, when you are ready to get a quote.
The Ideal Order For Your Kitchen Remodel
Rachel has experienced hundreds of kitchen remodels performed by experienced contractors as well as DIYers. The most common problem is always the steps are not completed in the Ideal Order. Rachel has put together a free PDF and video for you to help you get on the right path whether you are an expert or a beginner!


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Institut for Energikonvertering og -lagring
Bygning 310, rum 240
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Få tilsendt vCard på e-mail.
During the last 10 years he played a
leading role in a new cross-disciplinary research fields in the
area of functional materials for energy application. He has
nucleated and matured in Denmark an area now known as magnetic
refrigeration which includemagnetic materials; magnetocaloric
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area which he started at DTU is high temperature thermoelectric
materials and in particular design of novel properties and
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Fedora 24 + Bumblebee + CUDA + Theano
It's very frustrating when you want to try something out quickly and lose your entire day… But at least next time it will be easier. I hope.
This is a guide on how to run Keras (Theano powered neural network Python library) on CUDA with an NVIDIA Optimus laptop, with Fedora 24 and Bumblebee.
Bumblebee working
Get Bumblebee working, that is the easy part. Use the nonfree drivers, there are very good instructions here:
Afterwards verify that Bumblebee is working with a quick:
optirun glxgears
Install CUDA
Get CUDA from here:
The version for Fedora 21 is the latest one, and it will work. Unpack it after downloading it with:
sh cuda_7.5.18_linux.run -extract=/path/to/somewhere
You will get three files, the driver (which you don't need), CUDA toolkit and some samples. Install the toolkit and...
Continue reading →


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Continuous ECG Telemetry Monitoring in Cardiac Rehabilitation: Matching the need to the patient
Tuesday, July 24, 2018
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This webinar will review the historical use of continuous ECG monitoring in early outpatient (Phase 2) cardiac rehabilitation, including safety and relevant insurance regulations. The rationale and research regarding non-ECG monitored cardiac rehabilitation will be discussed. Considerations for moving a patient off of continuous ECG monitoring will be explored along with who should remain on telemetry during exercise. Lastly, contemporary models of titrated ECG monitoring will be presented and discussed.
Course Objectives:
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I just can't believe how popular the CD has become and the fab reviews I received from Vivien Sabel (Baby Communication Expert and Author) and Elizabeth James (Founder of Jellybeans Music and Music Bags). We have even had agreement to sell it in a NI retail outlet soon!
It is a great accompaniment to our classes, particularly for those attending wrigglers classes as we sing every single one of the 26 songs during the term.
I will never forget the day I recorded it....Traipsing up to the recording studio armed with tissues, as I had a stinking cold. Then being escorted to the recording suite, feeling like I was a 'proper musician'. I felt a bit stupid though when I got the big headphones on and stood in front of the mike with the techno guy outside mixing and doing his stuff, particularly when I started belting out 'Twinkle Twinkle Little Star'. I bet I was the talk of that studio for a long time. I will never forget the techno guy's face when I started singing 'Little baby isn't happy, 'cos he has a dirty nappy'!.... He looked like he expected someone to jump out and say 'ha! ha! Gotcha....at any stage!
We do also have another programme, Movers Classes, for older toddlers. For this programme I wrote many new songs, including themed songs like 'I Like Playing with My Toys', 'What's the Weather Like Today' and a few new parachute songs, because of course Daisy Doggy not only loves to hear the music, she loves to play with the parachute too!
For a couple of years now I have been promising to produce a second CD and this Thurs I am taking the first step to keeping that promise! I am going to be on a tight schedule with 3 hrs to record 26 tracks, with.....a cold again!!! I have been told that I sounded like one of the Smurfs on the first CD, so I think it is fitting that I have a cold again to keep with the Smurfy theme, lol!
I am very excited about this CD as I know the content is good, even if I do say so myself!
- It will include themed songs from the classes for topics like Animals, Opposites, Transport, Playtime, Manners and Behaviour.
- There will also be some well known favourites including Little Peter Rabbit, Head Shoulders Knees and Toes, Row your Boat and 5 Little Ducks.
- This CD will include a big favourite from our classes 'My Hands Can Clap Like This', recorded with permission from Dawn Errington (Signing Tots).
- A new song will also debut on the CD that even the Sign2Music teachers haven't heard yet - Clap Your Hands, Just Like Signing Sandy. This is an adaptation of one of the songs recorded by Rowena (Rhythm Express) who does our backing tracks!


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Description: Vintage large rhodium plated unfurled fan, the front folds have been gold plated. The top edge of the fan is set with a row of small clear rhinestones. The bottom of the fan has a curved row of invisibly set emerald green square rhinestones with a ruffle decoration over the folds set with clear rhinestones. The ribbon at the bottom is also set with clear rhinestones. All elements set with clear rhinestones have been left silver tone, to contrast with the gold plating everywhere else.
Size: 2 3/4" x 2"
Condition: Very good, some dull and/or yellow rhinestones