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Do you need sex without obligations? CLICK HERE - registration is completely free!
June 23, Our members are currently taking extra precautions to assure they are covid free and safe to date. If you are a single woman, or man, you can join our free database and get matched with our commitment-minded millionaires. Please make sure to review our TOP 5 Tips before submitting your complimentary application. You can book Patti Stanger by emailing Assistant millionairesclub Its users have included doctors, lawyers, CEOs, Hollywood celebrities, professional models and other well known personalities.
Millionaire dating programme
Stanger continued about the refractory client, a persnickety woman in her lates who pays for dating advice as part of Ms. Stanger said. When the client arrived, she perched on one of the lip chairs to wait while Ms.
Find out when Millionaire Age Gap Love is on TV, including Series 1-Episode 2. and year-old millionaire Lyn, who is accustomed to dating men 40 years.
Published: Mar 21, If there was ever greater proof that we now have too many TV channels filled up with rubbish it is Millionaire Matchmaker, writes Ben Lammas. This American monstrosity of a show, which has now found a home on British television, is the definition of trash television. It is a dating show, but one that would have Cilla Black hiding behind the sofa.
The series, which has somehow run for six seasons in America, follows the loud-mouthed Patti Stanger, owner of the Beverly Hills-based "Millionaire's Club" dating service, as she matches single wealthy people with closely compatible dates. As is the norm for American shows of this kind it moves at a million-miles-an-hour. Patti storms into her office, freshly botoxed and demands to see her latest clients. This time around it was millionaire year-old Jason, the vice president of a medical training school, who we are introduced to as he is driven around New York in a limousine.
Patti quickly susses them both out and orders her staff to round up some guys and girls pronto. What follows is a kind of audition stage where some poor candidates for the dates are paraded before Patti and her assistants who hurl insults at them like "you've got a bad stripper look"and "are you really 27, you look 37"; she even bluntly shouts at one cowering girl "you're ugly" before turning to her assistant and yelling "if you don't bring me some hot girls I am going to fire your ass.
But no sooner has she as much as thrown anyone 'too ugly' for her clients out of the window of her office than Patti suddenly comes over all pious and demands vain David has an ugly makeover so he can appreciate what it is like to be dismissed on looks alone.
Patti Stanger ends her eight season run on Bravo's Millionaire Matchmaker
The Channel 5 show was switched out for a double bill of Royal Family analysis and footage. Viewers will have to wait just a little longer to go behind the curtain and under the sheets of football power couple John and Carolyn Radford's 10 year relationship. Due to air last night January 9 the programme was switched at the last minute to make way for a two hour double bill of Royal Family analysis and footage, following the shock news Prince Harry and Meghan Markle would be standing down as senior royals.
Married in , the pair met when Carolyn, 37, landed a job at John's firm, One Call Insurance, two years before. It only took 12 months for the Mansfield natives to fall in love and become engaged.
Not Showing this week. Sorry The Millionaire Matchmaker is not on in the following week. The Gadget Show · BBC Proms · Lodgers for Codgers.
The next step is to input more personal details such as your country, zip code, annual income, height, body type, ethnicity, and relationship status. All details are required to be disclosed. After typing in the required basic details, you would be required to upload your profile photo which would be pending for approval. After a successful upload, you are required to write paragraphs about yourself and the person you are looking for.
These paragraphs should have a minimum of 50 characters. Interaction in MillionaireMatch can be quite challenging for free members. This is because the dating website only allows for its premium members to send messages. Free members could only reply to messages sent to them. However, free members can send winks and add interesting users to their favorites list.
All users can only wink after they have viewed 5 full profiles. Only 50 winks are allowed to be sent daily. This ensures that users only send genuine likes.
'Millionaire Matchmaker' Patti Stanger answers viewers' quarantine dating questions
Account Options Meld aan. Nuwe vrystellings. Voeg by wenslys.
The program is hosted by Patti Stanger, and here's the full episode. Here are 18 things you may or may not have known about Querrey, which he.
Patti Stanger Alberto E. The self-described love guru is a third-generation matchmaker and exceptionally proud of her ability to make love connections. After graduating college and spending a short time as the Marketing Director of a dating website, Patti Stanger decided to launch her own matchmaking company. She called it The Millionaires Club. Not just anyone could join, of course. Only clients with an impressive bank account and yes, they required proof of financial status were offered coveted spots in the club.
With her special combination of tough love and a knack for predicting who would have chemistry, Patti Stanger successfully helped launch hundreds of relationships. We are back! Season 2 milliondollarmatchmaker airs august 4th 10 pm!
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The year-old 'cupid', returned to the show back in for his third season as Hollywood matchmaker Patti Stranger's "left-hand man" but he has now confirmed he's leaving the programme for good. Speaking to Scott McGlynn on his podcast 'The Scott McGlynn Show', when asked when the new series was returning, David spilled: "I've actually left the show, it was my last season just gone.
David landed his first stint on the show several years when he joined as the matchmaker's date before he returned to assist rich clients alongside Stranger. Just last year the show moved channels from Bravo to WEtv and David started the season with Stanger's Justin Jedlica, the self-proclaimed "Human Ken Doll", who has undergone nearly plastic surgery operations.
Aside from his TV work, David has a radio station and blog called Finding-cupid.
This American monstrosity of a show, which has now found a home on British television, is the definition of trash television. It is a dating show.
There is an art to successfully dating a millionaire man. You have to know where to meet a millionaire and how to get him interested in dating you. To do so, your job is learning how to look the part, act the part, and be the part. As a Scottsdale Matchmaker I deal with successful men looking for their life partners. But as a Phoenix Dating Coach, I know these dating pointers can help you date all men — not just the wealthy men! Men in general are very visual, and wealthy men often have even higher standards for beauty than average men.
A millionaire man has the prerogative to be very choosy about the kind of woman he dates. To pass the test, here are a few simple rules:. Here are some traits you can work on cultivating:. I had a millionaire client who met a woman working in an upscale store. She was unhappy in her career and constantly complained to him. After a few months of her complaining, he realized he did not want this negative person in his life and ended their relationship.
The Millionaire Matchmaker: the unravelling of feminism?
Not the results you were expecting? Click here to search our entire site Press Esc to close Please enter a valid email address. Subscribe to our Daily Highlights Newsletter:. Tweets by tvguideshows. Inside Legoland: A World Strike Mon 31 Aug Gardening Together with
Matchmaker Patti Stanger, who has moved her operations from Los Angeles to New York, struggles to keep her own personal life on track while running her matchmaking business, Millionaire's Club, dedicated to helping wealthy and demanding men and.
It was up to Patti to show her that there are good men out there who want to love Traci for who she is. She was able to open up to Patti who, in turn, found two men who would treat Traci the way she deserves to be treated. WE were rooting for Traci and were excited when she found a match in Edwin. Patti helped me realize that I had a ton of resentment that I was holding onto including abandonment issues. I thought I was ready for love, but after being vulnerable and letting down my guard, I found it to be harder than I thought.
Patti taught me I need to work on bettering myself. On the first date, I admit I was initially a tad bit nervous. Sarah is still in school and we travel to see each other… So far so good! To watch full episodes, you must have a cable provider that supports WE tv's full episode service and you must have WE tv as part of your cable package. Are you sure you want to deactivate your account?
Millionaire Age Gap Love
The Millionaire Matchmaker is an American reality television series on Bravo that premiered on January 22, , and is hosted by Patti Stanger. In July , Stanger announced that she had left the network after eight seasons. Millionaire followed the "pioneering" matchmaking show Confessions of a Matchmaker.
Millionaire Matchmakers very own matchmaker David Cruz has confirmed he wont be returning to the next series of the US dating show.
Jane Higgins. Everyone wants to meet and fall in love with the millionaire of her dreams! Millionaire Matchmaker is an American reality show on ITV that gives you this vicarious pleasure and the fact that most of the people on the show seem fairly obnoxious only adds to the fun. Patti Stanger is the presenter of the show as well as the owner of a dating agency, the Millionaires Club based in Beverly Hills.
She attempts to match wealthy clients with compatible dates and this show follows the events of their dating process, to see if they can find true love. Unsurprisingly the focus of the programme is that the rich men are the target for not so rich pretty women. The millionaires have paid a lot of money to join the agency and in most cases, the reasons that these so called eligible bachelors are still single become obvious once they go on their dates.
Watch Millionaire Matchmaker via youriplayer.
Millionaire Confessions: Traci & Derek
En cliquant sur Lecture, vous acceptez nos Conditions d'Utilisation. Sous-titres Sous-titres. Audio Audio. New Challenges for Patti: Longtime lovebirds in the office, a drop-dead gorgeous lady millionaire and a middle-age looker looking for someone much younger. Patti deals with extreme opposites: Laurence is a total extrovert, while Andrew is a gorgeous introvert.
Deadline: You're just about to show Quiz to an audience for the first time, how are that Left Bank is owned by Sony and Sony also owns the rights to Millionaire? Up-to-date lists for broadcast, cable and streaming series.
By Karen Mizoguchi for MailOnline. Patti Stanger introduced many hopeless romantics on her hit show Millionaire Matchmaker. But on Friday, the newly single year-old love liaison announced she's ending her relationship with the Bravo network after eight seasons. The TV personality took to her Twitter page to share the news: 'After 8 seasons I've decided to leave Bravotv Thank u 4 the great ride.
Stay tuned for exciting news as I move to a wonderful new network! Scroll down for video.
Find love now.
Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Zurbriggen and Elizabeth M. Zurbriggen , Elizabeth M. Morgan Published Psychology Sex Roles.
This show has no redeeming value. It is not even good to watch for any amusement. On one episode, the matchmaker visited the home of one of her client.
Sam Querrey is an unassuming year-old tennis pro. He is ranked No. And he is some kind of unlucky at love. Thus he set out to find a significant other—or at least a decently romantic date—on Millionaire Matchmaker , presented by Bravo, the cable channel that launched a hundred substandard housewives' careers. The program is hosted by Patti Stanger, and here's the full episode. Here are 18 things you may or may not have known about Querrey, which he may or may not regret having shared on national TV:.
He travels, he leaves, and it fizzles out.


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Business News
Bitcoin is performing better than gold
The world's most popular digital coin has been having a good run, outperforming gold prices by +0.86% in the last 12 months.
Bitcoin has been climbing lately, amid the COVID-19 pandemic that has been disrupting global financial markets. The world's most popular digital coin has been having a good run, outperforming gold prices by +0.86% in the last 12 months.
This confirms its status as a viable store of value in times of quantitative easing and economic crisis. So far in 2020, bitcoin is up about 23%. For instance, today, it moved from $8,739.6 to $8,861 up 1.39%.
READ ALSO: Nigerians purchasing power to drop in 2020 – CBN survey
"Bitcoin may have a couple more attempts at breaking back towards the psychological $10,000 level, but right now it seems the fundamentals could easily support that," wrote Edward Moya, a senior market analyst at Oanda.
About 21 million bitcoins will ever be produced and presently, there are about 18.355 million bitcoins in circulation, according to CoinMarketCap.com.
READ MORE: Startups under Google Launchpad Accelerator Africa class 3 have raised N3.24 billion
"We're bullish over the next 12 months and expect prices may continue moving up into the [halving] and possibly after," wrote David Grider lead digital strategist at Fundstrat in a Thursday report. Fundstrat's 12-month outlook for bitcoin is $14,350.
Bitcoin traded at about $8,944 on Saturday evening, though it surged as high at $9,150 earlier in the day.
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Tech News
MTN, Vodacom launched 5G in sub-Saharan Africa in 2020 – GSMA Report
Vodacom and MTN launched their first major 5G networks in Sub-Saharan Africa in 2020, according to the GSMA 2020 report.
The Global System for Mobile Communications (GSMA) 2020 report revealed that Vodacom and MTN launched their first major 5G networks in Sub-Saharan Africa in 2020.
The telecoms operators offered 5G mobile and fixed wireless access (FWA) services in several locations across South Africa – this appears to be a welcome development, as the South African government had already assigned temporary spectrum in the 3.5 GHz range in the wake of the Covid-19 pandemic.
Obviously, the proximate opportunity to be harnessed for the 5G in South Africa is to use FWA to bridge the gap in fixed broadband connectivity for homes and businesses.
According to the report, there has been 5G trial runs in almost all the countries in Sub-Saharan Africa, including Gabon, Kenya, Nigeria and Uganda but the possibility of mass deployment of the 5G network is still not guaranteed, as there are significant levels of unused 4G capacity. Also, the 4G adoption rate is still relatively low, creating opportunities for the operators to increase their stakes in 4G.
As a boost to mop up the unused 4G capacity, the partnership between Safaricom and Google to finance the acquisition of 4G smartphones, provides the desired momentum as low-income consumers pay for 4G devices in convenient and flexible daily installments.
According to the report, it is expected that over the next five years, the number of smartphone connections in Sub-Saharan Africa will almost double to reach 678 million by the end of 2025 — an adoption rate of 65%.
What you should know
- It is expected that by 2025, there will be a little below 30 million mobile 5G connections in Sub-Saharan Africa, equivalent to almost 3% of total mobile connections.
- The mobile market in the region will reach several important milestones over the next five years: half a billion mobile subscribers in 2021, 1 billion mobile connections in 2024, and 50% subscriber penetration by 2025.
- The achievement of these critical milestones would be predicated on the operators' commitment in providing reliable infrastructural networks across the region.
- Between 2019 and 2025, the operators in the region would have expended/invested about the sum of $52billion in infrastructure rollouts.
- The GSMA represents the interests of mobile operators worldwide, uniting more than 750 operators with almost 400 companies in the broader mobile ecosystem, including handset and device makers, software companies, equipment providers and internet companies, as well as organizations in adjacent industry sectors.
Financial Services
Stamp Duty on Nigerian Stock market transactions pegged at 0.08% from December 7
The NSE has given clarifications on the public notice released by the FIRS, itemizing contract notes at an ad valorem rate of 0.08%.
The Nigerian Stock Exchange has given clarifications on the public notice released by the Federal Inland Revenue Service (FIRS) in July, itemizing contract notes at an ad valorem rate of 0.08% up from 0.075%, effective 7th December 2020.
The circular released by the Nigerian Stock Exchange reads:
"In reference to the Public Notice in the Business Day Newspaper of Monday, 20 July 2020, captioned 'Clarification of Administration of Stamp Duties in Nigeria' issued by the Federal Inland Revenue Service (FIRS) (A copy is attached as Appendix A for ease of reference).
"The Public Notice provided, amongst other things, information on dutiable instruments and the applicable flat or ad valorem rates, with Contract Notes 1 itemized at an ad valorem rate of 0.08%. As you know, this is at variance with the current rate of 0.075% administered in the Nigerian Capital Market."
To that extent, Dealing Members of the Nigerian Stock Exchange are to note the following:
- Effective December 7, 2020, the Central Securities Clearing System Plc. (CSCS) will adjust its system to implement the automated deduction of the Stamp Duty rate of 0.08%.
- Dealing Members are required to immediately engage their software vendors for the required adjustments to their technology applications, to reflect the 0.08% rate ahead of the effective date of 7 December 2020.
- Dealing Members are required to communicate the changes above to their clients immediately, ahead of the effective date.
What you should know
Nairametrics revealed that the FIRS listed at least 50 types of transactions that are eligible for stamp duty deductions.
Some of the listed chargeable transactions include bank deposit or transfer, loan agreement, Memorandum of Understanding (MoU), sales agreement, will, tenancy/lease agreement, and all receipts.
The FIRS noted that the recently inaugurated FIRS Adhesive Stamp is not the same as the postage stamp administered by NIPOST for the purposes of delivery of items and documents.
The Stamp Duties Act, 19391 defines Contact Notes as "the note sent by a broker or agent to his principal, or by any person who, by way of business, deals, or holds himself out as dealing, as a principal in any stock or marketable securities, advising the principal, or the vendor or purchaser, as the case may be, of the sale or purchase of any stock or marketable security, but does not include a note sent by a broker or agent to his principal where the principal is himself acting as broker or agent for a principal."
See the circular below:
Covid-19: UK to approve Pfizer, BioTNech vaccine, to start immunization December 7
The UK government is set to approve the COVID-19 vaccine developed by BioNTech SE and Pfizer Inc next week.
The global reception of the Covid-19 vaccine developed by Pfizer Inc in collaboration with BioNTech following the positive outcome of its phase 3 trial, seems to have intensified as it is set for approval by the UK medical regulator.
According to Reuters, a report from Financial Times on Saturday suggests that deliveries would commence within hours of the authorization with the first immunizations using the BioNTech and Pfizer vaccine possibly taking place from December 7.
The UK Prime Minister, Boris Johnson, had earlier in the day, named Nadhim Zahawi, who is the current junior business minister, as the minister responsible for the deployment of COVID-19 vaccines.
The UK government has placed an order for 40 million doses of the Pfizer and BioNTech vaccine, which has been found to be 95% effective in the final analysis of the phase 3 trials in preventing the spread of a virus that has killed over 1.4 million people across the world with its devastating impact on the global economy.
The UK government had on November 20, formally asked its medical regulator, the Medicines and Healthcare Products Regulatory Agency (MHRA), to conduct a study of the Pfizer-BioNTech COVID-19 vaccine with a view to determining its suitability, the first step in making it available outside the United State
The government which had secured 100 million doses of the Covid-19 vaccine developed by AstraZeneca and University of Oxford had also asked the regulator on Friday to assess the vaccine for a possible rollout before Christmas.
What you should know: The US drugmaker, Pfizer Inc, on November 18, 2020, announced that a final analysis of clinical-trial data of its experimental Covid-19 vaccine, which it is developing in collaboration with BioTNech, showed it was 95% effective, thereby paving the way for the company to apply for the first U.S. regulatory authorization for a coronavirus shot.
Pfizer said they had no serious safety concerns in a trial that involves almost 44,000 participants as their vaccine protected people of all ages and ethnicities.

subset: commoncrawl


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Gov't adds five areas for foreign investment
The areas are Munmak in Gangwon, Yeosu in South Jeolla, Gunsan in North Jeolla, Pyeongtaek in Gyeonggi and Hwaseong in Gyeonggi.
If all goes as planned, the five new areas will attract about 1.2 trillion won ($1.1 billion) in foreign investment and create 1,159 jobs.
In Munmak, German medical company Artificial Life and three similar businesses are planning to set up branches. That is estimated to generate about $68 million in investment and 580 jobs over the next five years.
In Yeosu, Italy's Versalis will launch a joint venture with Korea's Lotte Chemical to produce synthetic rubber, starting in 2017. They plan to invest a total of 543 billion won.
Japanese material developer Toray Industries will invest 305 billion won to build facilities to produce polymer products in Gunsan, one of the government-run free economic zones.
Optical-film maker Nitto Denkko plans to invest 178 billion won to produce films for touch panels for smartphones and tablet PCs.
American gas producer Air Products and Chemicals plans a $63.3 billion investment.
Korea received its largest-ever foreign direct investment last year of $16.2 billion. This year, however, the total FDI amount isn't likely to top that, with the ministry earlier setting a goal of $13.5 billion.
"We forecast the actual amount would exceed the goal, but not beyond last year's record," Chun said.
BY SONG SU-HYUN [firstname.lastname@example.com]
More in Economy
Consumer price gains pick up speed in November
Life expectancy up 7 months for Koreans born in 2019
OECD knocks tenth of a point off Korea's 2020 growth
Bos taurus philately
$504 billion budget for 2021 ready for passage


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NOTICE: Many events listed here have been canceled or postponed due to the Covid-19 emergency. It is best to call ahead or check with organizer's websites to verify the status of any local event.
Sharing the knowledge of over 15 years of international experience in a 3-day seminar including naturopathic approaches, protocols, application of supplements/naturopathic formulations, patient reporting and progress tracking software, practice management software, cloud apps, standard operating procedures, and much more. To assure due attention to every seminar participant, enrollment is limited to 6 participants only — no exceptions. A three-day, intense learning seminar for health practitioners addressing ways to support and help restore health in children with an autism spectrum disorder. Seminar conducted by Dr. Andre Kulisz, PhD, ND(US), DNM(EU), ACN, accredited with the European Union and expert of neurotoxicity and chronic inflammatory conditions at the European Commission. This seminar offers a great opportunity for practitioners to expand to the field of abruptly growing need and is intended to equip practitioners with a stand-alone practice module to be either included in the existing practice or practiced independently. The seminar provides all that is needed to start, including patient assessment, treatment protocols, software tools, business instructions, recommended optional instrumentation, and more. Expect a busy program and be prepared to immerse yourself in the area that is considered not treatable by allopathic medicine and others that benefit from the "management" of autistic children. Three-month start-up assistance included. Certificates of Completion issued by World-Wide Naturopathic Health Service will be awarded to participants that complete all three days of the seminar. The cost of the seminar is $3,950.00. Organizers offer a special $500.00 introductory discount and an additional discount of $500.00 for early-bird payments by November 6, 2020, for a total early bird price of $2,950.00. Hotel (3 nights), breakfast, lunch, dinner, and snacks are included. Suggested attire is business casual. For more information send an email to firstname.lastname@example.com with "Seminar 22-3" in the subject line or call 210-858-9143. Early Bird - $2450.00 Regular Admission - $2950.00
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Our deli Sicilian olives grow on the foothills of Mount Etna. Imported by us because one thing we know is that when something is grown in the Italian sunshine it tastes so much better. Nocellara Etnea is a a variety of green olive with a fleshy texture and uniform fruit size; the volcanic soil gives them their intense flavour.
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A screenshot of the Xbox dashboard taken from my iPhone.
Daniel Van Growth/CNET
With the Xbox Sequence X across the nook, Microsoft has up to date its iOS Xbox app. With the replace comes an enormous new characteristic in distant play that allows you to stream video games out of your Xbox One to your iPhone or iPad. Good timing too, with 4 new iPhones about to go on sale.
First, to mood your pleasure, this isn't Venture xCloud. That service permits you to play video games on Android telephones from the cloud, whereas this distant play operate as a substitute casts video games out of your Xbox One to your cellphone. Mainly, you may nonetheless have to be in your own home to make use of distant play.
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Subscribe to the CNET Now publication for our editors' picks of an important tales of the day.
To make use of distant play, you may want a stable web connection and a Bluetooth controller on your iPhone/iPad. Microsoft is not first to allow you to stream its video games onto iOS, with Sony's Distant Play transferring to iPhones and iPads final yr.
Nonetheless, it is a notable step towards Microsoft's cloud-based future for the Xbox platform. Whereas Sony is specializing in piling up exclusives for the PlayStation, just like the upcoming Ratchet and Clank: Rift Aside and Spider-Man: Miles Morales video games, Microsoft is specializing in Recreation Move and Venture xCloud. The previous is a Netflix-esque service that allows you to play a whole lot of video games for a $10 a month, whereas the latter permits you to stream Xbox and PC video games from the cloud in your Android units.
The brand new app permits you to obtain video games to your console out of your iPhone or iPad, so it is able to play while you get house.
Screencap by Daniel Van Growth/CNET
Distant play for iOS can also be of be aware as a result of Apple, notoriously protecting of its platform, has prior to now barred Microsoft from increasing xCloud to iOS and iPadOS. Microsoft is reportedly hoping to carry xCloud to iOS and iPadOS by means of a web-based app, reasonably than by means of the tightly managed App Retailer, however Apple kicking Epic Video games off its platform reveals how kindly it takes firms attempting to bypass its guidelines.
The brand new iOS Xbox app, as with its Android counterpart, permits you to handle your console out of your cellphone. You may delete video games to release reminiscence and, extra importantly, obtain video games to the console so that they're able to play while you get house. It's also possible to share movies and screenshots from the app to your social media, for those who're into that form of factor.


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In this course, you will :
- From one-off customer satisfaction surveys to brand tracking surveys that are administered on a continuous basis, they provide the information that marketers need to understand how their products, services and brands are seen by consumers.
- In Analytic Methods for Survey Data, learners will become familiar with established statistical methods for converting survey responses to insights that can support marketing decisions.
- Techniques discussed include factor analytics, cluster analysis, discriminant analysis and multi-dimensional scaling.


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I had the pleasure of photographing this beautiful family once again! This time we headed to the foothills of Orange County. The evening was spent chasing the light in Casper's Wilderness Park.
I started photographing in the ocean 13 years ago. I bought my first water housing to take with me on a sailing trip from San Diego, CA to Hiva Oa, French Polynesia and beyond. It was an Ewa Marine housing that I used with my Nikon 8008s and I shot with Fujichrome 100-36 frames at a time. Naturally when my children started to spend hours in the ocean, I would want to photograph them there too.
I was inspired by…
It had been 3 years since I photographed this gorgeous family from Pennsylvania and when I heard that they wanted me to be there again when they came to visit Coronado, I was thrilled to find out that they had added to their bevvy of beautiful girls!!
I have spent the last 8 years photographing my children in the ocean and pool to explore their seasoning in the water. From their love of small wavelets lapping at their toes to being inside the 6′ waves in the shore break, I have watched and witnessed and documented every step of their learning process.
I have been inspired by Wayne Levin for many years. His 'Canoe Paddlers' and 'Surfers' caught my attention…
Birth to age 1
Age 1 to Age 2
Age 2 to Age 3
Age 3 to Age 4
Age 4 to Age 5
From the series 'Rite of Water'
When we step into the water, it is a feeling like no other. It is smooth and cool. In order for us to thrive in such an unusual environment, we must allow ourselves to be vulnerable. I spent the better part of my life experiencing vulnerability in the ocean: from swimming in the shore break to surfing at the height of a summer swell to swimming off our sailboat in the ocean's depths beyond…
It's always an honor to work with CHOC Children's Hospital and this year was the most inspirational project I've worked on with them. They selected 4 children age 1-16 who wrote about what they hope for their future and why. From a parent's perspective for their one year old to a teenagers dream to cure skin cancer, every child in the campaign is a real person with hopes and dreams for their future. I've also included some behind the…
I had so much fun photographing this beautiful family bright and early before the crowds at Disneyland. The light was fabulous and they had special places all picked out for their portraits. What a great way to start the day!!
I have been busy with both my kids' schools lately. Besides the usual homework and after school activities, I also commit quite a bit of time to helping in both their classes. I feel it helps show them how important school is to us and how proud we are of them to be students.
I spend most of my time at my daughter's preschool. I have always enjoyed photographing…


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Species of Culex (Diptera: Culicidae) belonging to the subgenus Culiciomyia were collected in partially logged areas and in surrounding pristine forest (Talangaye Forest) in the Nguti Subdivision in the South-West Region of Cameroon. Mosquitoes were collected mainly by sweep netting through forest floor vegetation. Morphological species identification of African Culiciomyia relies almost exclusively on the structure of the male genitalia and the shapes of comb scales on the maxillary palpi of males. Other features of males and the habitus of females are largely indistinguishable between the species of this subgenus. In total, seven currently described species and three new species were collected in the forest. The males of the three new species are described and named as Culex apicopilosus Cornel Mayi, sp. n., Culex lanzaroi Cornel Mayi, sp. n. and Culex pseudosubaequalis Cornel Mayi, sp. n. More detailed descriptions of males of the other currently known species that were collected in the Talangaye Forest and pictorial keys to the males of all Afrotropical species of Culiciomyia, including the new species, are provided.
AssuntosCulex , Culicidae , Animais , Camarões , Feminino , Florestas , Masculino
Sabethes (Sabethoides) glaucodaemon was described for the first time by Dyar Shannon (1925) based on the adult female. Later, descriptions of the male genitalia and parts of the fourth-instar larva and pupa were published by other authors. No one has described the female genitalia or made a complete description of the larva and pupa. The aim of this study was to redescribe Sa. glaucodaemon in the adult stage, including the male and female genitalia, and the pupa and fourth-instar larva. All stages are illustrated. Distinctions from Sa. (Sbo.) tridentatus are discussed.
AssuntosCulicidae , Animais , Feminino , Genitália Feminina , Genitália Masculina , Larva , Masculino , Pupa
Seven genera and more than 98 species of mosquitoes (Diptera: Culicidae) are found in southwestern Asia (Harbach 1988). The most recent checklist of Iranian mosquitoes includes 69 species representing either seven or 11 genera depending on the classification used for aedines (Azari-Hamidian et al. 2019). Orthopodomyia pulcripalpis (Rondani) was the most recent genus and species to be added to the Iranian mosquito fauna (Azari-Hamidian et al. 2017).
AssuntosCulicidae , Animais
Decamyia Dyar is a subgenus of Wyeomyia Theobald with three valid species. Wyeomyia rorotai Senevet, Chabelard Abonnenc, a species originally described rather briefly in the subgenus Dendromyia, is without subgeneric position in the genus. In the present work, we redescribe Wy. rorotai in all life stages and formally define its taxonomic placement in the subgenus Decamyia by combining morphological and molecular analyses based on the mitochondrial cytochrome c oxidase subunit I gene. We also show that Decamyia is a rather homogeneous group of four species, i.e. Wy. ulocoma (Theobald), Wy. pseudopecten Dyar Knab, Wy. felicia Dyar Núñez Tovar and Wy. rorotai, the immature stages of which almost exclusively inhabit the flower bracts of Heliconiaceae.
AssuntosCulicidae , Heliconiaceae , Animais
The known cicada fauna of the Republic of Trinidad and Tobago is identified. Calyria hyperochelabrys n. sp., Carineta trinidadensis n. sp., and Herrera grammosticta n. sp. are described as new. The first records of Fidicinoides descampsi Boulard Martinelli, 1996, Proarna hilaris (Germar, 1834), Guyalna bogotana (Distant, 1892a), Majeorona aper (Walker, 1850), Taphura boulardi Sanborn, 2011a, Carineta matura Distant, 1892b, and Herrera nigropercula Sanborn, 2020b are provided. The first reports for the genera Guyalna Boulard Martinelli, 1996, Majeorona Distant, 1905d, Calyria Stål, 1862, Carineta Amyot Audinet-Serville, 1843 and Herrera Distant, 1905c and the tribes Parnisini Distant, 1905e and Carinetini Distant, 1905c are represented by the records for the new species and new records. The first records of Proarna hilaris (Germar, 1830) for Anegada, Antigua, Grenada, Canoun Island, Mayreau Island and Union Island in the Grenadines, Little St. James, Little Thatch Island, Montserrat, Mosquito Island, and St. Kitts, Proarna palisoti (Metcalf, 1963a) for Navarro Island, Majeorona aper (Walker, 1850) for Grenada, and Ariasa albimaculosa Sanborn, 2016c for Bonaire are provided to increase the known distributions of these species and the Caribbean fauna. The currently known cicada fauna for Trinidad is increased here to 15 species representing ten genera, four tribes and two subfamilies. Only Quesada gigas (Olivier, 1790) is known currently from Tobago.
AssuntosCulicidae , Hemípteros , Animais , Trinidad e Tobago
BACKGROUND: Jamestown Canyon virus (JCV) is a mosquito-borne orthobunyavirus that causes acute febrile illness, meningitis, and meningoencephalitis, mainly among adults. JCV is widely distributed in North America and the number of JCV cases in the U.S. has increased in recent years. Therefore, the central nervous system disease caused by JCV can be considered a potentially re-emerging viral disease. However, the seroprevalence of JCV is unknown in Japan. The purpose of this study is to evaluate the seroprevalence of JCV in the Japanese population. METHODS: We used an IgG enzyme-linked immunosorbent assay (IgG-ELISA) with JCV-infected cell-lysates and/or a neutralizing (NT) antibody assay. The cut-off value of IgG-ELISA was determined using IgG-ELISA to analyze serum specimens from 37 healthy Japanese donors. IgG-ELISA was validated by assessing its sensitivity and specificity, using 38 human serum samples previously tested for the presence or absence of antibodies against JCV and snowshoe hare virus (SSHV), in an in-house NT antibody assay conducted by the Public Health Agency of Canada. The seroepidemiological study was performed using IgG-ELISA and NT antibody assay to analyze 246 human serum samples from the serum bank of the National Institute of Infectious Diseases (NIID) in Japan. RESULTS: The cut-off value of IgG-ELISA was determined at 0.20, based on the mean (- 0.075) and standard deviation (0.092) values using Japanese donors' sera. The sensitivity and the specificity of IgG-ELISA determined using 25 JCV-positive and 4 JCV-negative serum samples were 96 and 100%, respectively. Analysis of the 246 Japanese serum samples revealed that no specimen showed a higher value than the cut-off value of IgG-ELISA, and no sample tested positive by the NT antibody assay. CONCLUSIONS: Our results showed that JCV is not circulating significantly in Japan. To the best of our knowledge, this is the first report to demonstrate the seroprevalence of JCV in the general population in Japan.
AssuntosAnticorpos Antivirais/imunologia , Vírus da Encefalite da Califórnia/imunologia , Encefalite da Califórnia/epidemiologia , Ensaio de Imunoadsorção Enzimática/métodos , Testes de Neutralização/métodos , Adolescente , Adulto , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/sangue , Criança , Pré-Escolar , Culicidae/virologia , Encefalite da Califórnia/virologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Lactente , Recém-Nascido , Japão/epidemiologia , Masculino , Pessoa de Meia-Idade , Prevalência , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , Adulto Jovem
The genus Uranotaenia (Diptera: Culicidae) has been well documented in Madagascar where it includes 73 species, 89.4% being endemic. However, one problem is that most species are morphologically similar in the adult stage. Here, 713 Uranotaenia specimens collected in the tropical forests of Anorana and Maromizaha between 2008 and 2014 were examined. Using the dichotomous keys for the Uranotaenia fauna of Madagascar published in 2004, three species were identified: Uranotaenia neireti (220), Ur. alboabdominalis (110) and Ur. mayottensis (28). The other specimens (355) were not identifiable and were classified as Uranotaenia sp1. Using wing morphometry, the four taxa were classified into four morphogroups. Within the Uranotaenia sp1 group, specimens from the Anorana forest and those from the Maromizaha forest overlapped. This result suggests that wing morphometric traits could be a good marker to distinguish Uranotaenia species in Madagascar.
AssuntosCulicidae , Animais , Madagáscar , Asas de Animais
Anthropogenic environments provide favorable conditions for some species, which is especially true of mosquitoes that present eclecticism at the moment of choice for the site of oviposition. In the present study, the diversity of mosquitoes was assessed by providing plastic containers, bamboo internodes, and tires in a forest, the forest edge, and peridomicile environments in a rural settlement area. Eighteen sampling points were chosen, delimited by a buffer of 200 m, placed in three environments: forest, forest edge, and peridomicile. In each environment, larvitraps were installed, separated by a minimum distance of 7 m and 1 m from the ground. A total of 10,131 immature mosquitoes of 20 species were collected. The most abundant species was Culex urichii (29.5%), followed by Trichoprosopon digitatum (27.1%), and Cx. (Melanoconion) spp. (10.4%). There was a difference in the composition of immature mosquito populations between larvitraps (p < 0.0005), and the plastic container hosted a greater diversity of species, whereas tires presented a greater abundance of individuals. The forest, forest edge, and peridomicile environments were also different with regard to diversity of immature mosquito populations (p < 0.0010). The forest edge was the environment with the greatest diversity of species, followed by the peridomicile and forest environments. In the forest and peridomicile, plastic container larvitraps had the greatest diversity, whereas the forest edge tire presented the largest number of individuals. Further, tire larvitraps collected the largest number of individuals in all environments. Ten species associated with the bamboo internode and tire were identified. The preference of species for artificial larvitraps, such as the plastic container and tire, even in wild environments was noted. These artificial objects may represent a risk factor for the population living in this region, as all vector species found in the study were present in plastic containers and tires.
AssuntosBiodiversidade , Culicidae/classificação , Culicidae/fisiologia , Ecossistema , Animais , Brasil , Feminino , Florestas
The lack of effective vaccines for many endemic diseases often forces policymakers to rely on non-immunizing control measures, such as vector control, to reduce the massive burden of these diseases. Controls can have well-known counterintuitive effects on endemic infections, including the honeymoon effect, in which partially effective controls cause not only a greater initial reduction in infection than expected, but also large outbreaks during control resulting from accumulation of susceptibles. Unfortunately, many control measures cannot be maintained indefinitely, and the results of cessation are poorly understood. Here, we examine the results of stopped or failed non-immunizing control measures in endemic settings. By using a mathematical model to compare the cumulative number of cases expected with and without control, we show that deployment of control can lead to a larger total number of infections, counting from the time that control started, than without any control-the divorce effect. This result is directly related to the population-level loss of immunity resulting from non-immunizing controls and is seen in a variety of models when non-immunizing controls are used against an infection that confers immunity. Finally, we examine three control plans for minimizing the magnitude of the divorce effect in seasonal infections and show that they are incapable of eliminating the divorce effect. While we do not suggest stopping control programs that rely on non-immunizing controls, our results strongly argue that the accumulation of susceptibility should be considered before deploying such controls against endemic infections when indefinite use of the control is unlikely. We highlight that our results are particularly germane to endemic mosquito-borne infections, such as dengue virus, both for routine management involving vector control and for field trials of novel control approaches, and in the context of non-pharmaceutical interventions aimed at COVID-19.
AssuntosControle de Doenças Transmissíveis/métodos , Doenças Endêmicas/prevenção & controle , Programas de Imunização , Animais , Número Básico de Reprodução , Infecções por Coronavirus/prevenção & controle , Culicidae , Vacinas contra Dengue/uso terapêutico , Política de Saúde , Humanos , Insetos Vetores , Modelos Teóricos , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Saúde Pública , Rubéola (Sarampo Alemão)/prevenção & controle , Vacina contra Rubéola/uso terapêutico , Estações do Ano , Dengue Grave/prevenção & controle , Vacinas Virais/uso terapêutico
Pyrethroids are a class of highly effective, broad-spectrum, less toxic, biodegradable synthetic pesticides. However, despite the extremely wide application of pyrethroids, there are many problems, such as insecticide resistance, lethal/sub-lethal toxicity to mammals, aquatic organisms or other beneficial organisms. The objectives of this review were to cover the main structures, synthesis, steroisomers, mechanisms of action, anti-mosquito activities, resistance, photodegradation and toxicities of pyrethroids. That was to provide a reference for synthesizing or screening novel pyrethroids with low insecticide resistance and low toxicity to beneficial organisms, evaluating the environmental pollution of pyrethroids and its metabolites. Besides, pyrethroids are mainly used for the control of vectors such as insects, and the non-target organisms are mammals, aquatic organisms etc. While maintaining the insecticidal activity is important, its toxic effects on non-target organisms should be also considered. Pyrethroid resistance is present not only in insect mosquitoes but also in environmental microorganisms, which results in anti-pyrethroids resistance (APR) strains. Besides, photodegradation product dibenzofurans is harmful to mammals and environment. Additionally, pyrethroid metabolites may have higher hormonal interference than the parents. Particularly, delivery of pyrethroids in nanoform can reduce the discharge of more toxic substances (such as organic solvents, etc.) to the environment.
AssuntosInseticidas/toxicidade , Piretrinas/toxicidade , Animais , Culicidae/efeitos dos fármacos , Resistência a Inseticidas/fisiologia , Inseticidas/química , Inseticidas/metabolismo , Mosquitos Vetores , Fotólise , Piretrinas/química , Piretrinas/metabolismo
A novel orbivirus (genus Orbivirus, family Reoviridae), designated Yonaguni orbivirus (YONOV), was isolated from bovine blood collected on a subtropical island of Japan in 2015. The YONOV genome (20,054 nucleotides in total) has a coding arrangement similar to those of mosquito-borne orbiviruses. YONOV has a close genetic relationship to mosquito-borne orbiviruses, especially to Mobuck virus (MBV), which was isolated in North America. However, YONOV and MBV share less than 74% nucleotide sequence identity in the major subcore protein (T2) coding sequence, which satisfies the criterion for species demarcation. It is still uncertain whether YONOV should be assigned to a novel species in the genus Orbivirus.
AssuntosGenoma Viral , Orbivirus/classificação , Orbivirus/isolamento & purificação , Filogenia , Infecções por Reoviridae/veterinária , Proteínas Virais/genética , Animais , Bovinos/virologia , Culicidae/virologia , Japão , Fases de Leitura Aberta , Infecções por Reoviridae/virologia , Análise de Sequência de DNA
Ross River virus (RRV) is the most common and widespread arbovirus in Australia. Epidemiological models of RRV increase understanding of RRV transmission and help provide early warning of outbreaks to reduce incidence. However, RRV predictive models have not been systematically reviewed, analysed, and compared. The hypothesis of this systematic review was that summarising the epidemiological models applied to predict RRV disease and analysing model performance could elucidate drivers of RRV incidence and transmission patterns. We performed a systematic literature search in PubMed, EMBASE, Web of Science, Cochrane Library, and Scopus for studies of RRV using population-based data, incorporating at least one epidemiological model and analysing the association between exposures and RRV disease. Forty-three articles, all of high or medium quality, were included. Twenty-two (51.2%) used generalised linear models and 11 (25.6%) used time-series models. Climate and weather data were used in 27 (62.8%) and mosquito abundance or related data were used in 14 (32.6%) articles as model covariates. A total of 140 models were included across the articles. Rainfall (69 models, 49.3%), temperature (66, 47.1%) and tide height (45, 32.1%) were the three most commonly used exposures. Ten (23.3%) studies published data related to model performance. This review summarises current knowledge of RRV modelling and reveals a research gap in comparing predictive methods. To improve predictive accuracy, new methods for forecasting, such as non-linear mixed models and machine learning approaches, warrant investigation.
AssuntosInfecções por Alphavirus/epidemiologia , Infecções por Alphavirus/transmissão , Animais , Austrália/epidemiologia , Clima , Culicidae/virologia , Previsões , Humanos , Incidência , Vírus do Rio Ross/isolamento & purificação , Tempo (Meteorologia)
We constructed an electric multi-rotor autonomous unmanned aerial system (UAS) to perform mosquito control activities. The UAS can be equipped with any of four modules for spraying larvicides, dropping larvicide tablets, spreading larvicide granules, and ultra-low volume spraying of adulticides. The larvicide module sprayed 124 µm drops at 591 mL/min over a 14 m swath for a total application rate of 1.6 L/ha. The tablet module was able to repeatedly deliver 40-gram larvicide tablets within 1.1 m of the target site. The granular spreader covered a 6 m swath and treated 0.76 ha in 13 min at an average rate of 1.8 kg/ha. The adulticide module produced 16 µm drops with an average deposition of 2.6 drops/mm2. UAS pesticide applications were made at rates prescribed for conventional aircraft, limited only by the payload capacity and flight time. Despite those limitations, this system can deliver pesticides with much greater precision than conventional aircraft, potentially reducing pesticide use. In smaller, congested environments or in programs with limited resources, UAS may be a preferable alternative to conventional aircraft.
AssuntosAeronaves , Culicidae , Monitorização de Parâmetros Ecológicos/instrumentação , Inseticidas/administração & dosagem , Controle de Mosquitos/instrumentação , Animais , Larva
BACKGROUND: Two billion long-lasting insecticidal nets (LLINs) have been procured for malaria control. A functional LLIN is one that is present, is in good physical condition, and remains insecticidal, thereby providing protection against vector-borne diseases through preventing bites and killing disease vectors. The World Health Organization (WHO) prequalifies LLINs that remain adequately insecticidal 3 years after deployment. Therefore, institutional buyers often assume that prequalified LLINs are functionally identical with a 3-year lifespan. We measured the lifespans of 3 LLIN products, and calculated their cost per year of functional life, to demonstrate the economic and public health importance of procuring the most cost-effective LLIN product based on its lifespan. METHODS AND FINDINGS: A randomised double-blinded trial of 3 pyrethroid LLIN products (10,571 nets in total) was conducted at 3 follow-up points: 10 months (August-October 2014), 22 months (August-October 2015), and 36 months (October-December 2016) among 3,393 households in Tanzania using WHO-recommended methods. Primary outcome was LLIN functional survival (LLIN present and in serviceable condition). Secondary outcomes were (1) bioefficacy and chemical content (residual insecticidal activity) and (2) protective efficacy for volunteers sleeping under the LLINs (bite reduction and mosquitoes killed). Median LLIN functional survival was significantly different between the 3 net products (p = 0.001): 2.0 years (95% CI 1.7-2.3) for Olyset, 2.5 years (95% CI 2.2-2.8) for PermaNet 2.0 (hazard ratio [HR] 0.73 [95% CI 0.64-0.85], p = 0.001), and 2.6 years (95% CI 2.3-2.8) for NetProtect (HR = 0.70 [95% CI 0.62-0.77], p < 0.001). Functional survival was affected by accumulation of holes, leading to users discarding nets. Protective efficacy also significantly differed between products as they aged. Equivalent annual cost varied between US$1.2 (95% CI $1.1-$1.4) and US$1.5 (95% CI $1.3-$1.7), assuming that each net was priced identically at US$3. The 2 longer-lived nets (PermaNet and NetProtect) were 20% cheaper than the shorter-lived product (Olyset). The trial was limited to only the most widely sold LLINs in Tanzania. Functional survival varies by country, so the single country setting is a limitation. CONCLUSIONS: These results suggest that LLIN functional survival is less than 3 years and differs substantially between products, and these differences strongly influence LLIN value for money. LLIN tendering processes should consider local expectations of cost per year of functional life and not unit price. As new LLIN products come on the market, especially those with new insecticides, it will be imperative to monitor their comparative durability to ensure that the most cost-effective products are procured for malaria control.
AssuntosMosquiteiros Tratados com Inseticida/economia , Inseticidas/economia , Controle de Mosquitos/métodos , Animais , Culicidae/efeitos dos fármacos , Vetores de Doenças , Características da Família , Seguimentos , Humanos , Resistência a Inseticidas/efeitos dos fármacos , Mosquiteiros Tratados com Inseticida/tendências , Inseticidas/farmacologia , Malária/prevenção & controle , Controle de Mosquitos/economia , Mosquitos Vetores/efeitos dos fármacos , Piretrinas/farmacologia , Tanzânia/epidemiologia
Exposure of adult mosquitoes to pyriproxyfen (PPF), an analog of insect juvenile hormone (JH), has shown promise to effectively sterilize female mosquitoes. However, the underlying mechanisms of the PPF-induced decrease in mosquito fecundity are largely unknown. We performed a comprehensive study to dissect the mode of PPF action in Aedes aegypti mosquitoes. Exposure to PPF prompted the overgrowth of primary follicles in sugar-fed Ae. aegypti females but blocked the development of primary follicles at Christopher's Stage III after blood feeding. Secondary follicles were precociously activated in PPF-treated mosquitoes. Moreover, PPF substantially altered the expression of many genes that are essential for mosquito physiology and oocyte development in the fat body and ovary. In particular, many metabolic genes were differentially expressed in response to PPF treatment, thereby affecting the mobilization and utilization of energy reserves. Furthermore, PPF treatment on the previtellogenic female adults considerably modified mosquito responses to JH and 20-hydroxyecdysone (20E), two major hormones that govern mosquito reproduction. Krüppel homolog 1, a JH-inducible transcriptional regulator, showed consistently elevated expression after PPF exposure. Conversely, PPF upregulated the expression of several key players of the 20E regulatory cascades, including HR3 and E75A, in the previtellogenic stage. After blood-feeding, the expression of these 20E response genes was significantly weaker in PPF-treated mosquitoes than the solvent-treated control groups. RNAi-mediated knockdown of the Methoprene-tolerant (Met) protein, the JH receptor, partially rescued the impaired follicular development after PPF exposure and substantially increased the hatching of the eggs produced by PPF-treated female mosquitoes. Thus, the results suggested that PPF relied on Met to exert its sterilizing effects on female mosquitoes. In summary, this study finds that PPF exposure disturbs normal hormonal responses and metabolism in Ae. aegypti, shedding light on the molecular targets and the downstream signaling pathways activated by PPF.
AssuntosAedes/efeitos dos fármacos , Culicidae/efeitos dos fármacos , Inseticidas/farmacologia , Metoprene/metabolismo , Esterilização , Animais , Ecdisterona/farmacologia , Corpo Adiposo/crescimento & desenvolvimento , Feminino , Glicogênio/metabolismo , Proteínas de Insetos/genética , Hormônios Juvenis/farmacologia , Ovário/crescimento & desenvolvimento , Piridinas , Interferência de RNA , Triglicerídeos/metabolismo
Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.
AssuntosFebre do Vale de Rift/diagnóstico , Animais , Mar Negro , Culicidae , Europa (Continente) , Genoma Viral , Humanos , Laboratórios , Mar Mediterrâneo , Febre do Vale de Rift/virologia , Vírus da Febre do Vale do Rift/genética , Ruminantes
BACKGROUND: Shifts have occurred in the epidemiological characteristics of Japanese encephalitis (JE), extending from the molecular level to the population level. The aim of this study was to investigate the seroprevalence of JE neutralizing antibodies in healthy populations from different age groups in Zhejiang Province, and to conduct mosquito monitoring to evaluate the infection rate of Japanese encephalitis virus (JEV) among vectors, as well as the molecular characteristics of the E gene of isolated JEV strains. METHODOLOGY/PRINCIPAL FINDINGS: A total of 1190 sera samples were screened by a microseroneutralization test, including 429 infants (28d-11m) and 761 participants (2y-82y). For those under 1 year old, the geometric mean titers (GMTs) of the JE neutralizing antibody was 9.49 at birth and significantly declined as the age of month increased (r = -0.225, P<0.001). For those above 1-year old, seropositive proportions were higher in subjects aged 1-3 years old as well as ≥25 years old (65%-75%), and relatively lower in subjects aged between 4-25 years old (22%-55%). Four or more years after the 2nd dose of JEV-L (first dose administered at 8 months and the second at 2 years of age), the seropositive proportion decreased to 32.5%, and GMTs decreased to 8.08. A total of 87,201 mosquitoes were collected from livestock sheds in 6 surveillance sites during 2015-2018, from which 139 E gene sequences were successfully amplified. The annual infection rate according to bias-corrected maximum likelihood estimation of JEV in Culex tritaeniorhynchus was 1.56, 2.36, 5.65 and 1.77 per 1000, respectively. JEV strains isolated during 2015-2018 all belonged to Genotype I. The E gene of amplified 139 samples differed from the JEV-L vaccine strain at fourteen amino acid residues, including the eight key residues related to virulence and virus attenuation. No divergence was observed at the sites related to antigenicity. CONCLUSIONS/SIGNIFICANCE: Zhejiang Province was at a high risk of JE exposure due to relatively lower neutralizing antibody levels among the younger-aged population and higher infection rates of JEV in mosquitoes. Continuous, timely and full coverage of JE vaccination are essential, as well as the separation of human living areas and livestock shed areas. In addition, annual mosquito surveillance and periodic antibody level monitoring are important for providing evidence for improvement in JE vaccines and immunization schedules.
AssuntosVírus da Encefalite Japonesa (Espécie)/genética , Encefalite Japonesa/epidemiologia , Encefalite Japonesa/virologia , Epidemiologia Molecular , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Anticorpos Neutralizantes , Criança , Pré-Escolar , China/epidemiologia , Estudos Transversais , Culex/virologia , Culicidae/virologia , Vírus da Encefalite Japonesa (Espécie)/classificação , Vírus da Encefalite Japonesa (Espécie)/imunologia , Vírus da Encefalite Japonesa (Espécie)/isolamento & purificação , Genes Virais/genética , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Mosquitos Vetores/virologia , Estudos Soroepidemiológicos , Adulto Jovem
BACKGROUND: Twenty-seven villages were selected in southwest Burkina Faso to implement new vector control strategies in addition to long lasting insecticidal nets (LLINs) through a Randomized Controlled Trial (RCT). We conducted entomological surveys in the villages during the dry cold season (January 2017), dry hot season (March 2017) and rainy season (June 2017) to describe malaria vectors bionomics, insecticide resistance and transmission prior to this trial. METHODS: We carried out hourly catches (from 17:00 to 09:00) inside and outside 4 houses in each village using the Human Landing Catch technique. Mosquitoes were identified using morphological taxonomic keys. Specimens belonging to the Anopheles gambiae complex and Anopheles funestus group were identified using molecular techniques as well as detection of Plasmodium falciparum infection and insecticide resistance target-site mutations. RESULTS: Eight Anopheles species were detected in the area. Anopheles funestus s.s was the main vector during the dry cold season. It was replaced by Anopheles coluzzii during the dry hot season whereas An. coluzzii and An. gambiae s.s. were the dominant species during the rainy season. Species composition of the Anopheles population varied significantly among seasons. All insecticide resistance mechanisms (kdr-w, kdr-e and ace-1 target site mutations) investigated were found in each members of the An. gambiae complex but at different frequencies. We observed early and late biting phenotypes in the main malaria vector species. Entomological inoculation rates were 2.61, 2.67 and 11.25 infected bites per human per month during dry cold season, dry hot season and rainy season, respectively. CONCLUSION: The entomological indicators of malaria transmission were high despite the universal coverage with LLINs. We detected early and late biting phenotypes in the main malaria vector species as well as physiological insecticide resistance mechanisms. These data will be used to evaluate the impact of complementary tools to LLINs in an upcoming RCT.
AssuntosAnopheles , Resistência a Inseticidas/genética , Malária Falciparum/transmissão , Mosquitos Vetores/genética , Animais , Anopheles/classificação , Anopheles/genética , Anopheles/parasitologia , Burkina Faso/epidemiologia , Culex/classificação , Culex/genética , Culex/parasitologia , Culicidae/classificação , Culicidae/genética , Culicidae/parasitologia , Ecologia , Genótipo , Humanos , Malária Falciparum/prevenção & controle , Controle de Mosquitos/métodos , Controle de Mosquitos/organização & administração , Mosquitos Vetores/classificação , Mosquitos Vetores/parasitologia , Plasmodium falciparum/isolamento & purificação , Estações do Ano
Gene drive technologies represent powerful tools to develop vector control strategies that will complement the current approaches to mitigate arthropod-borne infectious diseases. The characteristics of gene drive technologies have raised additional concerns to those for standard genetically engineered organisms. This generates a need for adaptive governance that has not been met yet because of the rapid rate of progress in gene drive research. For the eventual release of gene drive insects into wild populations, an international governance network would be helpful in guiding scientists, stakeholders, public opinion, and affected communities in its use. We examined the current institutions and governing bodies among various continents that could have an impact on gene drive governance or the potential to adapt to its future use. Possible governance strategies also are proposed that seek to bridge gaps and promote an ethically sound policy framework. Ideally, governance strategies should be developed before or at the same pace as gene drive research to anticipate field releases and maximize their impact as a public health tool. However, this is not likely to happen as it takes years to develop global accords, and some countries may choose to move ahead independently on the new technology.
AssuntosCulicidae/genética , Tecnologia de Impulso Genético/legislação & jurisprudência , Cooperação Internacional/legislação & jurisprudência , Controle de Mosquitos/legislação & jurisprudência , Mosquitos Vetores/genética , Agricultura/ética , Agricultura/métodos , Animais , Animais Geneticamente Modificados , Pesquisa Biomédica/ética , Pesquisa Biomédica/métodos , Tecnologia de Impulso Genético/ética , Humanos , Controle de Mosquitos/organização & administração , Saúde Pública , Característica Quantitativa Herdável
Temperature is widely known to influence the spatio-temporal dynamics of vector-borne disease transmission, particularly as temperatures vary across critical thermal thresholds. When temperature conditions exhibit such 'transcritical variation', abrupt spatial or temporal discontinuities may result, generating sharp geographical or seasonal boundaries in transmission. Here, we develop a spatio-temporal machine learning algorithm to examine the implications of transcritical variation for West Nile virus (WNV) transmission in the Los Angeles metropolitan area (LA). Analysing a large vector and WNV surveillance dataset spanning 2006-2016, we found that mean temperatures in the previous month strongly predicted the probability of WNV presence in pools of Culex quinquefasciatus mosquitoes, forming distinctive inhibitory (10.0-21.0°C) and favourable (22.7-30.2°C) mean temperature ranges that bound a narrow 1.7°C transitional zone (21-22.7°C). Temperatures during the most intense months of WNV transmission (August/September) were more strongly associated with infection probability in Cx. quinquefasciatus pools in coastal LA, where temperature variation more frequently traversed the narrow transitional temperature range compared to warmer inland locations. This contributed to a pronounced expansion in the geographical distribution of human cases near the coast during warmer-than-average periods. Our findings suggest that transcritical variation may influence the sensitivity of transmission to climate warming, and that especially vulnerable locations may occur where present climatic fluctuations traverse critical temperature thresholds.


text:
SQL Server has several methods of auditing logins and the simplest among
these is to change the audit level within SQL Server. Using traces through
Profiler or the set of trace stored procedures is another method, but most folks
gets started by configuring auditing in Enterprise Manager. Depending on the
auditing requirement, I may find myself using one or both methods. However, I'll
save tracing for another article.
Configuring Login Auditing
The most common way of configuring auditing for SQL server is through
Enterprise Manager. Bring up the SQL Server properties for the given server
(right-click on the server and choose Properties) and click on the Security tab.
You can set what kind of login events to audit under the Audit Level. I have
highlighted it in blue in Figure 1.
Figure 1: Setting Auditing Options
What about T-SQL? Unlike most SQL Server configuration settings, there isn't
an sp_configure option or other standard means of setting the audit level
in this manner. The audit level is stored in the registry and read when SQL
Server is started. When we make the setting change through Enterprise Manager it
updates one of the following registry values, depending on whether or not you
have a named instance or not (HKLM stands for HKEY_LOCAL_MACHINE):
|Default Instance:
|Named Instance:
|HKLM\Software\Microsoft\Microsoft SQL Server\<Instance
Here are the audit levels corresponding to the registry values:
|Audit Level
|Registry Value
|0x00000000 (0)
|0x00000001 (1)
|0x00000002 (2)
|0x00000003 (3)
If you're determined to make a change in audit level through T-SQL, the
undocumented stored procedures xp_regread and xp_regwrite permit
access to the registry. To read the current settings, use xp_regread.
|Default Instance:
|Named Instance
DECLARE @AuditLevel int EXEC master..xp_regread @rootkey='HKEY_LOCAL_MACHINE', @key='SOFTWARE\Microsoft\MSSQLServer\MSSQLServer', @value_name='AuditLevel', @value=@AuditLevel OUTPUT SELECT @AuditLevel
DECLARE @AuditLevel int EXEC master..xp_regread @rootkey='HKEY_LOCAL_MACHINE', @key='SOFTWARE\Microsoft\Microsoft SQL Server\<Instance Name>\MSSQLServer', @value_name='AuditLevel', @value=@AuditLevel OUTPUT SELECT @AuditLevel
Use xp_regwrite if you want to change the audit level. I will offer up
the standard warning about being very careful any time you touch the
registry. Unless you have a reason to touch the registry in such a way
(such as applying a script across multiple servers), it's best to use Enterprise
Manager. However, here is how to change the audit level using xp_regwrite.
|Default Instance:
|Named Instance
DECLARE @AuditLevel int SET @Auditlevel = <Audit Level Value> EXEC master..xp_regwrite @rootkey='HKEY_LOCAL_MACHINE', @key='SOFTWARE\Microsoft\MSSQLServer\MSSQLServer', @value_name='AuditLevel', @type='REG_DWORD', @value=@AuditLevel
DECLARE @AuditLevel int SET @Auditlevel = <Audit Level Value> EXEC master..xp_regwrite @rootkey='HKEY_LOCAL_MACHINE', @key='SOFTWARE\Microsoft\Microsoft SQL Server\<Instance Name>\MSSQLServer', @value_name='AuditLevel', @type='REG_DWORD', @value=@AuditLevel
Note: If you alter the audit level, be sure to stop and restart the
SQL Server. SQL Server reads this value from the registry during startup meaning
until there is a restart SQL Server will continue to perform at the old setting.
Success, Failure, or Both
SQL Server gives us the option of auditing login successes, failures, or
both. Realize that depending on your choices, there could be a lot of events
recorded. This is especially true of selecting success or both. In that case
every single successful new login will be recorded. If your SQL Server has a lot
of activity, there will be many, many events. Also, if you have automated
processes connecting to SQL Server with a SQL Server login, a single password
mismatch will generate quite a few errors on the failure side as well.
In all cases, ensure you audit at least to the level necessary. Carefully
consider anything more. The events will be written to both the SQL Server log
and the Windows application log, as I'll cover below. In the case of the SQL
Server log, realize that this will make the log larger in size. Ultimately the
log is a file on disk. The increase in events probably won't mean much as far as
disk space unless your exceptionally short on such, but it will impact anything
you use to view or parse the logs, including Enterprise Manager. For the Windows
application log, keep in mind that there is a setting to how large the log file
is and also the retention method. You may have to alter these settings to ensure
you don't lose any information.
Viewing Login Events - SQL Server Log
When a login event we're auditing for occurs, the information is recorded in
the SQL Server log. Failure events (Figure 2) just indicate what login attempted
to login:
Figure 2: Failure Events
Success events tell us a bit more information (Figure 3). If a connection was
made using Windows authentication, it's marked as a trusted connection.
Figure 3: Success Events
Getting the information out of the log using some sort of automated process
takes a bit more work than you might expect. While a particular log is being
written to by SQL Server, it is in use. That means you'll either need to cycle
the log so it's not in use or you'll need to use some method from within SQL
Server. However, if you're checking the log on a frequent basis, you're not
going to want to cycle the log. There is another option. The undocumented stored
procedure xp_readerrorlog gives us this access. We can parse the lines of
the error log for logon events by dumping the contents of the error log to a
temporary table and then looking for the substring of 'logon' in the full row.
|Parsing the SQL Server Log:
CREATE TABLE #errorlog ( rowID int IDENTITY, textRow varchar(4000), continuationRow int ) INSERT INTO #errorlog (textRow, continuationRow) EXEC master.dbo.xp_readerrorlog
SELECT textRow
FROM #errorlog
WHERE CHARINDEX('logon', textRow) > 0
ORDER by rowID
DROP TABLE #errorlog
Starting with this bit of code, you can modify it to build your own automated
process to report on the events recorded. Having a process collect and report
all the information saves us the trouble of having to hand-check each server. In
larger environments this is a must.
Viewing Login Events - Windows Application Log
SQL Server also records the information in the Windows Application log.
However, just looking at the log doesn't tell us if the logon event was a
success or failure. Nor does it tell us what the login was. Figure 4 shows an
example of a recorded audit event.
Figure 4: Audit Event in Application Log
Unfortunately, for both success and failure audits the Source will always by
MSSQLServer (or the Instance), the Category will always be (4) and the Event ID
will always be 17055. In order to gain more information we'll have to open up
the event log record and look at the description. Figure 5 shows a failed login
Figure 5: Failed Login Event
The same is true of a successful login attempt. We must look at the
description as in Figure 6.
Figure 6: Successful Login Event
Of course, many events are like this, so if you're already used to parsing
Windows event logs this is nothing new. There are all kinds of tools for getting
the information out of the event log; pick your favorite. If you don't have one,
take a look at PsLogList from SysInternals. It's a part of the PsTools
suite. PsLogList has the capability of outputting the event logs into a text
file. I've included a link in the Additional Resources section.
At some point you may be asked to set up some form of auditing on your SQL
Servers, if you haven't been already. There are a couple of ways to do this, but
simply adjusting the audit level on SQL Server is probably the easiest way to
start. Be careful when you start auditing to the first time as you may find a
lot of events being generated. These events are recorded both to the SQL Server
log and the Windows application log, therefore plan for an increase in the
number of records in both. You can also look in both locations to get the
results of your audit. Building an automated process to report the events is
easily done and helps reduce the workload of looking over the audit information.
Use what works best for you.
Additional Resources
- Useful Undocumented Stored Procedures by Alexander Chigrik - http://www.mssqlcity.com/Articles/Undoc/UndocExtSP.htm
- Undocumented T-SQL Tricks with Logs by Brian Knight - http://www.sqlservercentral.com/columnists/bknight/readpurgelog.asp
- SysInternal's PsLogList - http://www.sysinternals.com/ntw2k/freeware/psloglist.shtml
- SysInternal's PsTools Suite - http://www.sysinternals.com/ntw2k/freeware/pstools.shtml
2004 by K. Brian Kelley.
Author of Start to Finish Guide to SQL Server Performance
Monitoring (http://www.netimpress.com).


text:
from page 17
He cited the case of another Wall
Street Journal reporter, Jerry Seib, who
was held hostage in 1987 while
reporting from Iran. Seib was accused
of being a spy and a Jew, Walcott said,
although he is Roman Catholic.
But in Iraq today, Walcott said, "I
honestly believe that with the U.S.
gunning for Saddam, those distinc-
tions are rapidly shrinking. A Jewish,
Baptist or even a Muslim soldier is
likely to be treated equally badly by
this Iraqi regime.
"They are likely to lump all
Americans and Brits together as 'Jews
and Crusaders' in much the same way
that Osama bin Laden has."
One prominent Jewish journalist
says that given the current climate in
the Arab world, he would reconsider
reporting from certain locales. "After
the Daniel Pearl murder, I think jour-
nalists who are Jewish have to be very
careful about putting themselves in a
place where their lives will be at risk,"
said Pulitzer Prize-winner Joshua
Friedman, who began reporting from
the Arab world 40 years ago.
"I probably wouldn't go alone to
some of these places that I once went
to," said Friedman, former chairman
and current board member of the
watchdog Committee to Protect
Journalists. "We're vulnerable because
we're Jewish, and I'd never felt that
"There's a fever of anti-Semitism
today in Muslim countries and we
have to face it, right? It's true."
Still, not too many journalists would
be dissuaded, Friedman conceded. War
reporters, like soldiers, share a mind-set
of invincibility, rationalizing that it won't
be you but someone else who is arrest-
ed, gets shot or steps on a landmine.
"Most good journalists have an
adolescent view of their immunity to
danger," said Friedman, who won his
Pulitzer for IVewsday in 1985 for his
coverage of Ethiopia's famine.
One Jewish Army reservist, antici-
pating a call-up, says he's well aware
of what happened to the American
POWs in 1991 and to Daniel Pearl.
He admits to trying to suppress
thoughts of worst-case scenarios.
"I don't know any soldier who
thinks that you're going to be the
one who gets captured or killed,"
says Philip, a New England resident
who requested anonymity. "As far as
being a Jew, that's just something
extra. If the Iraqis find out I'm
Jewish and they torture me worse
than the other POWs, I'd probably
find it unbearable." ❑
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text:
- Is Thailand known for human trafficking?
- How does human trafficking in Thailand affect human rights?
- Is human trafficking legal in some countries?
- What is the biggest problem in Thailand?
- Is Thailand safe right now?
- Is YouTube banned in Thailand?
- Do I really need vaccines for Thailand?
- What country is known for human trafficking?
- What diseases can you get from Thailand?
- Can we drink tap water in Thailand?
- What is Bangkok belly?
- What is the most common STD in Thailand?
Is Thailand known for human trafficking?
Thailand's sex industry is a human trafficking destination.
Ethnic Thais are trafficked from poor areas of Chiang Rai, Nong Khai, and Phayao to the tourist areas.
The Thai government identified 720 trafficking victims of all types in 2015, up from 595 in 2014.
At least 151 of the 720 were victims of sex trafficking..
How does human trafficking in Thailand affect human rights?
This is affecting the human rights because it is enforcing a pain or sexual act on someone with out there consent. You are silencing their right to a freedom of speech. … The UN Inter-Agency Project on Human Trafficking (UNIAP) estimates that Thai authorities send home over 23,000 Cambodian trafficking victims a year.
Is human trafficking legal in some countries?
As of February 2018, 173 countries have ratified the United Nations Trafficking in Persons Protocol, of which UNODC is the guardian. Significant progress has been made in terms of legislation: as of 2012, 83 per cent of countries had a law criminalizing trafficking in persons in accordance with the Protocol.
What is the biggest problem in Thailand?
Fishing gear was the major cause, followed by disease and pollution. The death toll has hovered around 400 for three consecutive years and represents less than 10% of the 5,000 rare species found in Thailand's territorial waters.
Is Thailand safe right now?
From 4 July, Thailand is exempt from the FCO advice against all non-essential international travel. … The following advice within Thailand remains in place: The FCO advise against all but essential travel to areas within the provinces on the Thailand-Malaysia border, including: Pattani.
Is YouTube banned in Thailand?
During the week of March 8, 2007, YouTube was blocked in Thailand. Although no official explanation was given for the ban, many bloggers believed the reason for the blocking was a video of former Prime Minister Thaksin Shinawatra's speech on CNN. YouTube was unblocked on March 10, 2007.
Do I really need vaccines for Thailand?
The CDC and WHO recommend the following vaccinations for Thailand: hepatitis A, hepatitis B, typhoid, cholera, yellow fever, Japanese encephalitis, rabies, meningitis, polio, measles, mumps and rubella (MMR), Tdap (tetanus, diphtheria and pertussis), chickenpox, shingles, pneumonia and influenza.
What country is known for human trafficking?
The US on Thursday listed 10 countries it said are involved in state-sponsored human trafficking, including a host of its adversaries. Among the group are Afghanistan, China, Eritrea, Iran, North Korea, Russia, South Sudan, Syria, Turkmenistan and Venezuela.
What diseases can you get from Thailand?
Guide to Common Diseases in ThailandMalaria.Dengue.Japanese Encephalitis.Leptospirosis.Soil-transmitted worm diseases.Hepatitis B.Hepatitis A.HIV.More items…
Can we drink tap water in Thailand?
Don't drink tap water in Thailand, stick to boiled or treated water. Don't worry too much about the ice as there's an extensive network of ice factories which use purified water. It's easier for businesses to use the commercial ice rather than go to the expense of making their own.
What is Bangkok belly?
What causes Bangkok Belly. Generally known as traveler's diarrhea, this digestive tract disorder is caused by ingesting food or water that have been contaminated with organisms stemming from feces. These are most often caused by bacteria such as E. Coli, but viruses and parasites are to blame, too.
What is the most common STD in Thailand?
Chlamydial infection is one of the most common STDs. Like gonorrhoea, it is easily transmitted through sex without a condom, oral sex, anal sex and can even occur when you and your partner's genitals come into contact. Unlike some cases of gonorrhoea, this sneaky little thing often brings no symptoms.


text:
Charles L. Haan, 90 Charles L. Haan, 90, of Lafayette, died at 9:50 p.m. Tuesday, May 24, 2011, at St. Elizabeth Central. He was born May 19, 1921, in Tippecanoe County, to the late John H. and Mary Jenners Haan, and graduated from Battle Ground High School. He was a staff sergeant in the Army,…Continue Reading
Take Care,
Jim, Karen & Family


text:
Or as she is more often known as, SKCM Curry was named as Campaign Manager for the McKinney-Clemente Power to the People campaign. Curry had been actively seeking the Vice Presidential nomination, but seems quite content working in this role with the campaign.
There is a great post about Curry on the Wilderside that goes into great depth into her background and strength as an organizer. They got a video up as well, but just to be, well, "different", I'll embed here a year old video by the Wilders when Curry spoke to the GPUS at the Annual National Convention in Reading PA:


text:
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