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Artificial lipid membranes are often investigated as a replica of the cell membrane in the form of supported lipid bilayers (SLBs). In SLBs, the phase state of a lipid bilayer strongly depends on the presence of molecules such as cholesterol, ceramide, and physical parameters such as temperature. Cholesterol is a key molecule of biological membranes and it exerts condensing effect on lipid bilayers. In this paper, we demonstrate the influence of excess cholesterol content on a supported lipid bilayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (fluid-phase) using spectroscopic ellipsometry (SE) and coarse-grained (CG) molecular dynamics (MD) simulations. The results show the condensation effect due to cholesterol addition up to 30% and interleaflet decoupling at excess cholesterol beyond 30%. SE results show the separation of individual leaflets of the bilayer and influence of cholesterol on the biophysical properties such as thickness and optical index. CG simulations were performed at different ratios of DOPC:cholesterol mixtures to explore cholesterol-driven bilayer properties and stability. The simulations displayed the accumulation of cholesterol molecules at the interface of the lower and upper leaflets of the bilayer, thus leading to undulations in the bilayer. This work reports the successful application of SE technique to study lipid–cholesterol interactions for the first time.
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Abraham MJ, Murtola T, Schulz R, Páll S, Smith JC, Hess B, Lindah E (2015) Gromacs: High performance molecular simulations through multi-level parallelism from laptops to supercomputers. SoftwareX 1–2:19–25. https://doi.org/10.1016/j.softx.2015.06.001
Adhyapak PR, Panchal SV, Murthy AVR (2018) Cholesterol induced asymmetry in DOPC bilayers probed by AFM force spectroscopy. Biochim Biophys Acta Biomembr 1860:953–959. https://doi.org/10.1016/j.bbamem.2018.01.021
Ali MR, Kwan HC, Huang J (2007) Assess the nature of cholesterol-lipid interactions through the chemical potential of cholesterol in phosphatidylcholine bilayers. Proc Natl Acad Sci USA 104:5372–5377. https://doi.org/10.1073/pnas.0611450104
Almeida PF, Carter FE, Kilgour KM, Raymonda MH, Tejada E (2018) Heat capacity of DPPC/cholesterol mixtures: comparison of single bilayers with multibilayers and simulations. Langmuir 34:9798–9809. https://doi.org/10.1021/acs.langmuir.8b01774
Alwarawrah M, Dai J, Huang J (2010) A molecular view of the cholesterol condensing effect in DOPC lipid bilayers. J Phys Chem B 114(22):7516–7523. https://doi.org/10.1021/jp101415g
Arnarez C, Webb A, Rouvière E, Lyman E (2016) Hysteresis and the cholesterol dependent phase transition in binary lipid mixtures with the Martini model. J Phys Chem B 120:13086–13093. https://doi.org/10.1021/acs.jpcb.6b09728
Arwin H, Aspnes DE (1984) Unambiguous determination of thickness and dielectric function of thin films by spectroscopic ellipsometry. Thin Solid Films 113:101–113. https://doi.org/10.1016/0040-6090(84)90019-1
Azzam RMA, Bashara NM (1977) Ellipsometry and polarised light. North-Holland Pub, Amsterdam
Beneš M, Billy D, Hermens WT, Hof M (2002) Muscovite (mica) allows the characterisation of supported bilayers by ellipsometry and confocal fluorescence correlation spectroscopy. Biol Chem 383:337–341. https://doi.org/10.1515/BC.2002.037
Benzitouni S, Mahdjoub A, Zaabat M (2014) Spectroscopic ellipsometry characterization of thin films deposited on silicon substrate. J. New Technol. Mater. 4:138–142. https://doi.org/10.12816/0010318
Berendsen HJC, Postma JPM, Van Gunsteren WF, Dinola A, Haak JR (1984) Molecular dynamics with coupling to an external bath. J Chem Phys 81:3684–3690. https://doi.org/10.1063/1.448118
Bussi G, Donadio D, Parrinello M (2007) Canonical sampling through velocity rescaling. J Chem Phys. https://doi.org/10.1063/1.2408420
Carpenter TS, López CA, Neale C, Montour C, Ingólfsson HI, Di Natale F, Lightstone FC, Gnanakaran S (2018) Capturing phase behavior of ternary lipid mixtures with a refined martini coarse-grained force field. J Chem Theory Comput 14:6050–6062. https://doi.org/10.1021/acs.jctc.8b00496
Chiantia S, Kahya N, Ries J, Schwille P (2006) Effects of ceramide on liquid-ordered domains investigated by simultaneous AFM and FCS. Biophys J 90:4500–4508. https://doi.org/10.1529/biophysj.106.081026
Chiantia S, Ries J, Chwastek G, Carrer D, Li Z, Bittman R, Schwille P (2008) Role of ceramide in membrane protein organisation investigated by combined AFM and FCS. Biochim Biophys Acta Biomembr 1778:1356–1364. https://doi.org/10.1016/j.bbamem.2008.02.008
De Jong DH, Baoukina S, Ingólfsson HI, Marrink SJ (2016) Martini straight: boosting performance using a shorter cutoff and GPUs. Comput Phys Commun 199:1–7. https://doi.org/10.1016/j.cpc.2015.09.014
Dols-perez A, Fumagalli L, Gomila G (2014) Structural and nanomechanical effects of cholesterol in binary and ternary spin-coated single lipid bilayers in dry conditions. Colloids Surf B Biointerfaces 116:295–302. https://doi.org/10.1016/j.colsurfb.2013.12.049
Eeman M, Deleu M (2010) From biological membranes to biomimetic model membranes. Biotechnol Agron Soc Environ 14:719–736
El-Nasser HM (2017) Morphology and spectroscopic ellipsometry of PMMA thin films. Appl Phys Res 9:5–11. https://doi.org/10.5539/apr.v9n2p5
Feigenson GW (2006) Phase behavior of lipid mixtures. Nat Chem Biol 2:560–563. https://doi.org/10.1038/nchembio1106-560
Finot E, Markey L, Hane F, Amrein M, Leonenko Z (2013) Combined atomic force microscopy and spectroscopic ellipsometry applied to the analysis of lipid-protein thin films. Colloids Surfaces B Biointerfaces 104:289–293. https://doi.org/10.1016/j.colsurfb.2012.12.013
Hofsäss C, Lindahl E, Edholm O (2003) Molecular dynamics simulations of phospholipid bilayers with cholesterol. Biophys J 84:2192–2206. https://doi.org/10.1016/S0006-3495(03)75025-5
Howland MC, Szmodis AW, Sanii B, Parikh AN (2007) Characterisation of physical properties of supported phospholipid membranes using imaging ellipsometry at optical wavelengths. Biophys J 92:1306–1317. https://doi.org/10.1529/biophysj.106.097071
Janicek P, Niang KM, Mistrik J, Palka K, Flewitt AJ (2017) Spectroscopic ellipsometry characterisation of ZnO: Sn thin films with various Sn composition deposited by remote-plasma reactive sputtering. Appl Surf Sci 421:557–564. https://doi.org/10.1016/j.apsusc.2016.10.169
Johnson SA, Stinson BM, Go MS, Carmona LM, Reminick JI, Fang X, Baumgart T (2010) Temperature-dependent phase behavior and protein partitioning in giant plasma membrane vesicles. Biochim Biophys Acta Biomembr 1798:1427–1435. https://doi.org/10.1016/j.bbamem.2010.03.009
Jurak M (2013) Thermodynamic aspects of cholesterol effect on properties of phospholipid monolayers: Langmuir and Langmuir–Blodgett monolayer study. J Phys Chem B 117:3496–3502. https://doi.org/10.1021/jp401182c
Kamble S, Patil S, Kulkarni M, Murthy AVR (2019) Spectroscopic ellipsometry of fluid and gel phase lipid bilayers in hydrated conditions. Colloids Surf B Biointerfaces 176:55–61. https://doi.org/10.1016/j.colsurfb.2018.12.061
Khelashvili G, Kollmitzer B, Heftberger P, Pabst G, Harries D (2013) Calculating the bending modulus for multicomponent lipid membranes in different thermodynamic phases. J Chem Theory Comput 9:3866–3871. https://doi.org/10.1021/ct400492e
Kranenburg M, Smit B (2005) Phase behavior of model lipid bilayers. J Phys Chem B 109:6553–6563. https://doi.org/10.1021/jp0457646
Malmsten M, Kassner U, Winkler K, Schmidt A, Buddecke E, Saunders R, Siegel G (2004) An ellipsometry-based Alzheimer plaque mimic: effect of β-amyloid, lipoprotein identity and apolipoprotein e isoform. J Colloid Interface Sci 276:503–506. https://doi.org/10.1016/j.jcis.2004.05.044
Melo MN, Ingólfsson HI, Marrink SJ (2015) Parameters for Martini sterols and hopanoids based on a virtual-site description. J Chem Phys 143:243152. https://doi.org/10.1063/1.4937783
Mills TT, Toombes GES, Tristram-Nagle S, Smilgies D-M, Feigenson GW, Nagle JF (2008) Order parameters and areas in fluid-phase oriented lipid membranes using wide angle X-Ray scattering. Biophys J 95:669–681. https://doi.org/10.1529/biophysj.107.127845
Murthy AVR, Guyomarc'h Lopez FR (2016a) The temperature-dependent physical state of polar lipids and their miscibility impact the topography and mechanical properties of bilayer models of the milk fat globule membrane. Biochim Biophys Acta Biomembr 1858:2181–2190. https://doi.org/10.1016/j.bbamem.2016.06.020
Murthy AVR, Guyomarc'h F, Lopez C (2016b) Cholesterol decreases the size and the mechanical resistance to rupture of sphingomyelin rich domains, in lipid bilayers studied as a model of the milk fat globule membrane. Langmuir 32:6757–6765. https://doi.org/10.1021/acs.langmuir.6b01040
Murthy AVR, Guyomarc'h F, Paboeuf G, Vié V, Lopez C (2015) Cholesterol strongly affects the organisation of lipid monolayers studied as models of the milk fat globule membrane: condensing effect and change in the lipid domain morphology. Biochim Biophys Acta Biomembr 1848:2308–2316. https://doi.org/10.1016/j.bbamem.2015.06.014
Oh Y, Sung BJ (2018) Facilitated and non-gaussian diffusion of cholesterol in liquid ordered phase bilayers depends on the flip-flop and spatial arrangement of cholesterol. J Phys Chem Lett 9:6529–6535. https://doi.org/10.1021/acs.jpclett.8b02982
Pan J, Mills TT, Tristram-Nagle S, Nagle JF (2008) Cholesterol perturbs lipid bilayers nonuniversally. Phys Rev Lett 100:198103. https://doi.org/10.1103/PhysRevLett.100.198103
Pantelopulos GA, Straub JE (2018) Regimes of complex lipid bilayer phases induced by cholesterol concentration in MD simulation. Biophys J 115:2167–2178. https://doi.org/10.1016/j.bpj.2018.10.011
Parker A, Miles K, Cheng KH, Huang J (2004) Lateral distribution of cholesterol in dioleoylphosphatidylcholine lipid bilayers: cholesterol-phospholipid interactions at high cholesterol limit. Biophys J 86:1532–1544. https://doi.org/10.1016/S0006-3495(04)74221-6
Parrinello M, Rahman A (1981) Polymorphic transitions in single crystals: a new molecular dynamics method. J Appl Phys 52:7182–7190. https://doi.org/10.1063/1.328693
Petrache HI, Tristram-Nagle S, Gawrisch K, Harries D, Parsegian VA, Nagle JF (2004) Structure and fluctuations of charged phosphatidylserine bilayers in the absence of salt. Biophys J 86:1574–1586. https://doi.org/10.1016/S0006-3495(04)74225-3
Pradhan P, Aryal P, Attygalle D, Ibdah A-R, Koirala P, Li J, Bhandari K, Liyanage G, Ellingson R, Heben M, Marsillac S, Collins R, Podraza N (2018) Real Time Spectroscopic Ellipsometry Analysis Of First Stage CuIn1−xGaxSe2 growth: indium–gallium selenide co-evaporation. Materials (Basel) 11:145. https://doi.org/10.3390/ma11010145
Redondo-Morata L, Giannotti MI, Sanz F (2012) Influence of cholesterol on the phase transition of lipid bilayers: a temperature-controlled force spectroscopy study. Langmuir 28:12851–12860. https://doi.org/10.1021/la302620t
Richter RP, Bérat R, Brisson AR (2006) Formation of solid-supported lipid bilayers: an integrated view. Langmuir 22:3497–3505. https://doi.org/10.1021/la052687c
Marrink SJ, Jelger Risselada H, Yefimov S, Peter Tieleman D, de Vries AH (2007) The MARTINI force field: coarse grained model for biomolecular simulations. J Phys Chem B 111:7812–7824. https://doi.org/10.1021/JP071097F
Marrink SJ, de Vries AH, Mark AE (2003) Coarse grained model for semiquantitative lipid simulations. J Phys Chem B 108:752–760. https://doi.org/10.1021/JP036508G
Striebel C, Brecht A, Gauglitz G (1994) Characterisation of biomembranes by spectral ellipsometry, surface plasmon resonance and interferometry with regard to biosensor application. Biosens Bioelectron 9:139–146. https://doi.org/10.1016/0956-5663(94)80105-3
Sullan RMA, Li JK, Hao C, Walker GC, Zou S (2010) Cholesterol-dependent nanomechanical stability of phase-segregated multicomponent lipid bilayers. Biophys J 99:507–516. https://doi.org/10.1016/j.bpj.2010.04.044
Van Der Meulen SAJ, Dubacheva GV, Dogterom M, Richter RP, Leunissen ME (2014) Quartz crystal microbalance with dissipation monitoring and spectroscopic ellipsometry measurements of the phospholipid bilayer anchoring stability and kinetics of hydrophobically modified DNA oligonucleotides. Langmuir 30:6525–6533. https://doi.org/10.1021/la500940a
Veiga MP, Arrondo JL, Goñi FM, Alonso A (1999) Ceramides in phospholipid membranes: effects on bilayer stability and transition to nonlamellar phases. Biophys J 76:342–350. https://doi.org/10.1016/S0006-3495(99)77201-2
Vidawati S, Sitterberg J, Bakowsky U, Rothe U (2010) AFM and ellipsometric studies on LB films of natural asymmetric and symmetric bolaamphiphilic archaebacterial tetraether lipids on silicon wafers. Colloids Surf B Biointerfaces 78:303–309. https://doi.org/10.1016/j.colsurfb.2010.03.015
Wang Y, Gkeka P, Fuchs JE, Liedl KR, Cournia Z (2016) DPPC-cholesterol phase diagram using coarse-grained molecular dynamics simulations. Biochim Biophys Acta Biomembr 1858:2846–2857. https://doi.org/10.1016/j.bbamem.2016.08.005
Wassenaar TA, Ingólfsson HI, Böckmann RA, Tieleman DP, Marrink SJ (2015) Computational lipidomics with insane: a versatile tool for generating custom membranes for molecular simulations. J Chem Theory Comput 11:2144–2155. https://doi.org/10.1021/acs.jctc.5b00209
Zhang J, Xue R, Ong W-Y, Chen P (2009) Roles of cholesterol in vesicle fusion and motion. Biophys J 97:1371–1380. https://doi.org/10.1016/j.bpj.2009.06.025
The authors thank the DST-Inspire Faculty scheme from the Department of Science and Technology (DST) (DST/INSPIRE/04/2015/000207) for the funding of this work. The authors also thank DIAT for infrastructural support in implementing this project. SK thanks DIAT for a Ph.D. fellowship. The authors also thank Dr. Suwarna Datar for her support.
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Supplementary file1 Movie M1: Movie of 10% cholesterol system. PO4 beads of DOPC are indicated by pink color, cholesterol molecules by yellow and DOPC chains by cyan color. (MP4 69523 KB)
Supplementary file2 Movie M2: Movie of 10% cholesterol system. PO4 beads of DOPC are indicated by light gray color, cholesterol molecules by yellow and DOPC chains by cyan color. (MP4 180937 KB)
Supplementary file3 Movie M3: Movie of 50% cholesterol system depicting diffusion of cholesterol from 2 μs to 3 μs simulation time with a view along XY-plane. The cholesterol molecules are presented by beads, and color code indicates radial position from centrally localized (red) to outer sides (blue). (MPG 89007 KB)
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Kamble, S., Patil, S., Kulkarni, M. et al. Interleaflet Decoupling in a Lipid Bilayer at Excess Cholesterol Probed by Spectroscopic Ellipsometry and Simulations. J Membrane Biol 253, 647–659 (2020). https://doi.org/10.1007/s00232-020-00156-9
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- Lipid membranes
- Cholesterol
- Spectroscopic ellipsometry
- MARTINI force field
- Coarse-grained simulations


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Family & Relationships The Five Love Languages Might Seem Cliché. But, Damn, Are They Good for Your Marriage
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A restaurant in Sweden is taking the concept of social distancing to a whole other level: It will only serve a single guest each day.While bars, restaurants, and cafés in Sweden continue to serve seated customers, most of them have roped off every other seat or table in order to enforce social distance.
Love is patient and it is kind. But the way in which it is best expressed varies from person to person. We all respond to different types of affection, different love languages. In 1992, Gary Chapman, a senior associate pastor at Calvary Baptist Church in Winston-Salem, North Carolina, published The Five Love Languages: How to Express Heartfelt Commitment to Your Mate. In it, Dr. Chapman explains that the ways in which we express and experience love can be broken down to five "love languages": receiving gifts, quality time, words of affirmation, acts of service (devotion), and physical touch. It sounds cliché, almost sickly sweet. But since its release, The Five Love Languages has gone on to become one of the most purchased relationship advice books, remaining on the New York Times Best Seller List since 2009. Now, the phrase "love languages" is an unavoidable part of modern vernacular. There's a reason for that: It works.
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Why? Well, it's important to not only interrogate how we express love to those closest to us, but also understand what forms of love our significant others prefer. If we don't, we may not be acting in the way that's most advantageous.
"Many times we are expressing love to the other person, but we're doing it in a way that would make us feel loved if they expressed it to us," says Dr. Chapman. "We're not expressing in a way that would make them feel loved. So I think understanding that we do have different love languages, that there are different ways in which we perceive love emotionally, is important. So if I really want to be effective, then I'll choose to speak the love language of the other person."
How to Save Your Marriage With Ethical Non-Monogamy
A group of cutting-edge researchers, advocates, and writers believes that consensual non-monogamy should be a more considered option for couples.Before she met her husband, Beth was a free spirit floating through no-strings-attached hookups with a circle of male friends. In the early years of her marriage, she accepted that her days of untethered intimacy were over. The memories tugged at her sometimes, but her love for her husband and children always pulled more strongly. Sacrificing that youthful pleasure was worth what she'd gained in her new life. Besides she'd made a commitment to stay faithful to her husband. And that was that.
Dr. Chapman says that his understanding of the five love languages stemmed from years meeting with couples and listening to their problems. "When someone would sit in my office and say, 'I feel like my spouse doesn't love me,' I would ask, 'What did they want? What were they complaining about?' And their answers would fall into these five categories."
Learning how to speak these love languages can be difficult sometimes, Chapman says, because the everyday busyness of life, work, family, and responsibility stand in the way of focusing on a marriage or other relationship, and things tend to go on autopilot. In this scenario, resentment and unresolved conflicts remain below the surface and can keep us from using the love languages when they're needed. When someone is feeling angry or neglected, or is just coming off a disagreement, the last thing they want to think about is speaking anything resembling love to their partner.
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"People often will fail to speak the love language of the other person because they're not really feeling love," Chapman says. "They're feeling hurt, they're feeling put down. And if those things are unresolved, then you get a string of unresolved conflicts. And that's when people begin to think, 'Oh, we're not compatible.'"
For each person, their love language might be different, and it's important to find out what it is. Someone whose love language is words of affirmation, for example, will respond better to compliments and positive statements. If their love language is quality time, then its about making sure to give them time away from phones, TVs, and other distractions. It orients couples.
Finding out a partner's love language, Chapman says, simply comes down to listening to what they want and dialing into what it is they complain about the most or what they most often ask of their partner.
When he meets with couples for counseling, or even one partner, Chapman asks them to conduct a six-month experiment.
"I say, 'If you can discover your spouse's primary love language, would you be willing to try for the next six months to speak their love language at least once a week?'"
Worried Your Spouse Thinks You Have an Unhappy Marriage? This Will Help
The only way to know if your partner is unhappy about your marriage is to, well, talk to them about it. But it's what you say and how you say it that matters.Well, as a concerned husband, the most obvious answer is the correct one: talk to her. What's important, however, is how you approach the situation. What you say has to dig deeper than the standard line of interrogation, which involves a little delicacy and diplomacy on your part. Asking the right questions and saying the right things.
When they do, it works. It doesn't happen overnight, he says. It might be three months down the road. Eventually, however, the other person begins to reciprocate. "Because what you're doing is speaking to their primary language," he says. "You're connecting with them emotionally in a way you haven't been."
Once the love language a partner speaks is identified, there are many opportunities to get creative in how one chooses to express it. Chapman recalled one couple where the husband was in the military and his spouse knew that his primary love language was physical touch.
"You would think that physical touch would be impossible when you're half a world away," he says, "But she said, 'When he was deployed, I put my hand a sheet of paper and traced it, and then I mailed it to him with a note that said, 'Put your hand on my hand.' When he came home, he said, 'Gary, every time I put my hand on that paper, I felt her.'"
The five love languages aren't just for couples, Chapman notes. They can be used to relate to children, teenagers, even your in-laws, or any close relationship.
Chapman was speaking at a prison, and during the question and answer session, a man expressed thanks to him for coming. He told Chapman he realized his love language is physical touch, and that his mother never hugged them.
FYI, It's Totally Possible To Have Two Love Languages
Everything you need to know about figuring out what yours is—or, uh, are.First introduced by marriage counselor Gary Chapman in his book The 5 Love Languages, they "provide an easy way to curate a conversation about meeting one another's needs in a relationship," says Indigo Stray Conger, licensed marriage and family therapist and certified sex therapist. "Over time, couples can feel that they are both making a sincere effort to show love to their partner, yet somehow end up missing each other and feeling distant." Each encompasses a specific way a person may need to express or receive love.
"The only hug I ever remember getting from my mother was the day I left for prison," he told him. "But you gave those other languages and I realized that my mother spoke acts of service. She was a single mom. She worked two jobs. She kept our clothes clean. She kept food on the table." By the time the man had finished speaking, Chapman says he was actually crying, saying, 'My mama loves me.' "
The beauty of the five love languages is in its simplicity and the fact that it's never too late for someone to start learning a partner's language as long as they're willing to do the work.
"If you understand how important it is, then you want to find a way to keep it on the front burner," he says. "If that means putting a Post-It on your mirror reminding you, then so be it. But you want to keep it on the front burner so that you don't just drift. If we drift in a relationship, we drift apart. We don't ever drift together.
"You've got to put the oars in the water," he says. "You've got to be rowing."
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The post The Five Love Languages Might Seem Cliché. But, Damn, Are They Good for Your Marriage appeared first on Fatherly.
10 Signs That Your Marriage Will Last a Lifetime .
#1 is so important!


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Monthly Archives: March 2015
No, it isn't time for the Jews to leave Europe
"Is it time for the Jews to leave Europe?" is the title of the 11,000-word cover story in the latest issue of the Atlantic magazine written by the American Jewish journalist Jeffrey Goldberg. His answer? "Yes". Why, while purporting to write … Continue reading
Posted in Antisemitism, Europe Tagged America, antisemitism, Islamophobia, Leon Wieseltier 1 Comment
Israel's elections: more bad news for Palestinians and Europe's Jews
Bibi wins the Israeli elections. I guessed he would. To me, the tight opinion polls, that were even giving a substantial advantage to the Zionist Camp, could not take into account a last minute, knee-jerk return to the Likud out … Continue reading
Posted in Europe, Middle East Tagged 2-state solution, Elections 2015, Israel, Netanyahu, Palestine, Palestinians, racism, West Bank 5 Comments


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1 University Visvesvaraya College of Engineering
Part A
Chapter 1 : Introduction :
Model of a Digital communication system, Comparison of Digital & Analog Communication system,Bandwidth of Digital data, Base band Versus Band Pass. (3 Hours)
Review of sampling theorem, Sampling of low pass and band pass signals, practical aspect of sampling,Reconstruction of message from its samples, channel band width for PAM Signals, Generation anddetection of PAM. (5 Hours)
Messages, characters and symbols character coding, Formatting analog information –sampling.Quantization ,quantization –noise, Elements of PCM. Different PCM quantizer, Uniform and non-uniformquantization, companding , DPCM, PCM decoders. (6 Hours)
Principal , Noise In DM systems, Granular and slope overload in DM systems, ADM systems, Comparingof PCM and DM, Digital Multiplexers ,TDM – PCM Telephone system, CODEC. (4 Hours)
Line codes: Various type and their power spectra, regenerating repeater. (3 Hours)
2 University Visvesvaraya College of Engineering
Part B
Introduction – coding and decoding, inter symbol interference. Base band shaping optimum transmittingand receiver filter, correlative coding, Base Band M-array Pam systems, Adaptive equalization, Eyepatters scrambler and descrambler. (7 Hours)
Elements of a Band pass data transmission systems, correlation receiver, Matched filter receiver,Probability of error of matched filter. (6 Hours)
Modulation and demodulation performance of coherent and non- coherent BASK, Spectral properties ofBASK. (3 Hours)
Modulators and demodulators performance of coherent and non -coherent ASK system, Minimum shiftkeying , Binary PSK systems : Spectral properties of BPSK, BPSK modulators and demodulators ,Differential coherent demodulation of BPSK, Performance of matched filter detector, discriminatorDPSK, QPSK,MPSK. Introduction to spread spectrum techniques. (16 Hours)
Sam shanmugham : Digital and analog communication systems, John weily and sons Simon Haykin: Digital communication, Wiley international EDn,1989. Martin s Roden: Digital and data communication systems, prentice Hall, 1982. Leonw- couch – 2nd Analog communication systems,3rd Edn Maxwell macmillan International edn,1989. Bernard Sklar: Digital communications : PHI R Lathi : communication systems , Delhi , oxford Jonu G Prokis : Digital Communication.
3 University Visvesvaraya College of Engineering
EC 602: Microcontrollers:
Part A
8051 Microcontroller hardware, Pins and Ports circuits, external memory, counters and timers, serialdata I/O Interrupts. (7 Hours)
Understanding assembly language syntax of 8051, instruction syntax, addressing mode of 8051,extensive study of instruction set of 8051 and example programs. (14 Hours)
Memory devices, address decoding, 8/16/32/and 64bit Memory interfacing and dynamic RAMS. (6 Hours)
4 University Visvesvaraya College of Engineering
DMA operation ,DMA controller, shared bus operation ,Disk memory systems , Video display. (5 Hours)Chapter 8: Application of 8051 : Temperature sensors, stepper motor and Data acquisition system.
(2 Hours )References:
Brey B.B : The intel Microprocessor Architecture , Programming and interfacing , 4e PHI Ayala K J : The 8051 microcontroller , 2e,penram international Gaonker: Microprocessor Architecture and Programming Myke Predko : programming and customizing the 8051 microcontroller, TMH 2001
5 University Visvesvaraya College of Engineering
Chapter 1: Introduction:
Frequencies used, application, High frequency, limitation of conventional tubes (Qualitative only),klystron amplifier (two cavity) reflex klystron oscillator power output, efficiency, TWT: BWO: Cavitymagnetron –construction, principle of operation, performance characteristic and application. (6 Hours)
( Qualitative discussion only ) Tunnel, Pin , Varactor ,Schottky Barrier, Read,Gunn, TRAPATT,IMPATT ,Barritt construction and operation. Parametric amplifier using vacator, Manley-Rowe relationMOSFET,HEMT , oscillator using tunnel and Gunn diodes, PIN switch and modulator. (8 Hours)
E Plane-Plane ,Magic Tes & direction couples , contruction ,opetation and scattering matrixrepresentation, capacitive , inductive and E-Bends , Transitions and twits in wave guide, posts, tuningscrews , coupling probes and loops, stoltted line, Attenuators –fixed movable vane and rotary phaseshifter. (6 Hours)
Basic antenna parameter, patterns , beam array radiation intensity, Beam efficiency Directivity and Gain,Antenna aperture effective height , Radio communication link , Field from oscillating dipole , Antennafield Zones ,shape-Impedance considerations. (6 Hours)
Part B
Introduction power pattern theory and applications, Radiation intensity, Power patterns, Field Patterns,Phase patterns Arrays of two isotropic point sources, Non –isotropic similar point sources, Patternmultiplication, pattern synthesis, Non –isotropic dissimilar point sources, array of n-isotropic sources ofequal amplitude an spacing ,Null directions , array of two driven elements ( broadside cast end – firecase and general case with equal currents of any phase ) (7 Hours)
6 University Visvesvaraya College of Engineering
Loop antenna (General case), field comparison of short diploe and small loop, field pattern, radiationresistance, directivity, radiation efficiency, Q, Bandwidth and SNR .Helical antenna , Helical geometry ,Design consideration of monofilar axial-mode helical antenna, dipole arrays with parasitic elements ,theyagi-Uda array, axial – Mode pattern and Phase velocity of wave propagation on monofilar axial-modehelical antenna and wide and characteristics of monofilar axial-mode helical antenna. (03 Hours)
Radar Block diagram , radar frequencies, application of radar derivation , prediction, minimumdetectable signal, receiver noise, radar cross section of target, range ambiguities, CW and frequencymodulated radar: Doppler effect, CW radar, FW-CW radar, airborne Doppler navigation, multiplefrequency CW Radar. (5 Hours)
Block diagram, multiple or staggered pulse repetition frequencies, Pulse Doppler radar, racking radar,Tracking with radar, sequential lobing, conical scan, monopulse tracking radar, tracking in range,acquisition. (4 Hours)
Block diagram description description , duplexers, noise figure, low noise font end, mixers displays – PPI& A-scope , Randomes. (3 Hours)
7 University Visvesvaraya College of Engineering
Chapter 1:
Discrete source – Entropy , conditions for maximum value, definitions ,markov sources, Problems
(7Hours)Chapter 2:
Source coding- Properties of codes, Shannon, Shannon-Fano , Huffman Binary coding and efficiencycalculations, Non-binary coding – Huffman ternary and quaternary coding , efficiency calculations
(7 Hours)
Chapter 3:
Discrete channels – joint and conditional entropies, mutual information, capacity and extension ofchannels. (6 Hours)
Chapter 4:
Continuous channels – joint and conditional entropies, mutual information, capacity and extension ofchannels. (6 Hours)
Part B
Chapter 4
Error control codes-Block codes, Minimum distance considerations, standard array and syndromedecoding, block diagram for encoder and decoder (8 Hours)
Chapter 5
Binary cyclic codes- Generator polynomial systematic cyclic codes, circuits or Block diagram for Encoderand Syndrome calculation BCH, R-S and Goolay codes. (8 Hours)
Chapter 6
Convolutional codes- Block diagram, Encoding using time domain and transform domain approach, statediagram approach, code tree. (8 Hours)
8 University Visvesvaraya College of Engineering
Reference books:
9 University Visvesvaraya College of Engineering
Chapter 3: MOS Devices : Depletion and enhancement type transistors. Construction and working, transfer and output characteristics, Drain current equation, expressions for gm, ὠo, Cg, cox , currents gain factor etc. (4 Hours)
Chapter 6: Scaling: Different scaling models, merits of scaling, limitations of scaling, based design rules. (4 Hours)
10 University Visvesvaraya College of Engineering
Testing philosophy, VLSI testing process and test equipment, fault modeling, testing combinational and sequential logic, design for testability (DFT), Scan design techniques, BIST. (7 Hours) Chapter 8: Programmable devices: ROMS , PALS, PALS,PLDS and FPGAs , Type of FPGA s, Xilinx and Altera series of ICs . Two Typical ICs from each Vendor, Architecture and details, Design examples. (10 Hours)
Reference: Weste and Eshraghian : Principles of CMOS VLSI Design : A systems perspective pearson Educaton. J M Rabaey, A Chandrakasan, B . Nikolic: Digital integrated circuits : A design Perspective , pearson Education . Kang and Leblebici : CMOS digital integrated circuits , TATA Mc Graw- Hill Edition. Baker lin and Boyce: CMOS circuits design ,Layout and simulation, PHI EEE
11 University Visvesvaraya College of Engineering
1. Machines, Machine Languages, and Digital Logic: Classification of computers and their instruction, computer instruction sets. Informal description of the simple RISC computers, formal description of RISC using register transfer notation, RTN, Describing addressing modes with RTN, Register transfers and logic circuits from behaviour to hardware. (7 Hours) 2. Real Machines: Machine characteristics and performance, Machine performance, RISC versus CISC. A CISC microprocessor. The Motorola 68000. RISC architecture the SPARC. (6 Hours) 3. Processor Design: The design process. A I-bus micro-architecture of the SRC. Data path implementation. Logic design for the I-bus SRC. The control unit 2 and 3-bus processor designs. The machine reset. Machine exceptions. (7 Hours) 4. Processor Design: Advanced topics: Pipelining. Instruction: level parallelism – Microprogramming. (4 Hours) PART – B 5. Computer Arithmetic and the arithmetic unit: Number systems and Radix conversion. Fixed point arithmetic Semi-numeric aspects of ALU design. Floating point arithmetic. (4 Hours) 6. Memory System Design: Introduction, the components of the memory system. RAM structures the logic designer's perspective. Memory boards and module. Two level memory Hierarchy. The cache. Virtual memory. The memory subsystem in the computer. (7 Hours) 7. Input & Output: The I/O subsystem. Programmed I/O interrupts. Direct Memory Access (DMA). I/O data format change and error control. (6 Hours) 8. Peripheral Devices: Magnetic disk drives. Display devices. Printers. Input devices. Interfacing to the analog world. (7 Hours)
12 University Visvesvaraya College of Engineering
13 University Visvesvaraya College of Engineering
Find impulse response, step response, Ramp response & frequency response of a given system.
a) Linear convolution b) Circular convolution c) Verification of linear convolution using DFT d) Finding DT using FFT algorithms e) Finding the inverse FFT
Z – Transform
a) IIR digital filter design by impulse invariance method (both Butterworth & Chebshev filters). b) IIR digital filter design by Bilinear transformation ( both Butterworth & Chebshev filters). c) FIR Digital filter design by Windows.
a) Addition b) Subtraction c) Multiplication d) Division e) Factorial f) Generation of different wave forms g) Generation of Echo.


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China's 668 million internet users1 spent $589.61 billion (¥3.877 trillion) online in 20152 alone. What's more, that figure... Continue reading >
Chinese investors generated a record $23 billion in investment into Europe during 2015.1 That's nearly half of the US$51.6... Continue reading >
This year's Cannes Film Festival has unveiled a slew of new movies that will see China remain the world's most... Continue reading >
By 2025, Goldman Sachs predicts 220 million tourists from China will spend $450 billion annually.1 This makes Chinese one... Continue reading >
Did you know? The Mandarin language, or Pǔtōnghuà [普通话] in China, literally translates to "Common Speech". This is because Chinese... Continue reading >
Harcourt Developments (UK)
@juwailimited on twitter
For the latest news and analysis of the Chinese property buyer trends.
Juwai IQI, the real estate tech startup, claims it has turned profitable as of July, reports Tech in Asia (26 November 2020). The company said that the number of transactions conducted on...Read more >
Chinese investors are not wasting any time to buy properties in Portugal, reports Atlas Lisboa (25 November 2020), citing a South Morning Post article. Chinese buyers have rushed to purchase property in...Read more >
National residential property prices could climb 3.2 per cent over the next 12 months in the Philippines, but the financial district of Makati is expected to see weak prices and rents, reports...Read more >
Rents and residential prices in the Philippines are expected to start recovering by 2021, except in Makati City which may continue to see a decline for a longer period, reports Philippine Real...Read more >


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Discussion on Data Center Infrastructure
One year ago today HostDime opened its doors for its newest data center in João Pessoa, Paraíba, Brazil. The 20,000 square foot facility is one of the very few data centers in Northern Brazil. The four story building, designed and constructed by HostDime, is up to Uptime Institute's Tier III standards.
HostDime offers an array of software services to take your VPS or dedicated server to the next level. The following add-ons are perfect complements to servers with cPanel/WHM installed, and range from $5 to $12 a month.
ownCloud is a personal cloud that runs on your own server. Access your data wherever you are, and whenever you need it. This is also hugely beneficial to resellers; advertise one-click cloud storage directly to your clients.
Server management is the maintenance and overseeing of a web server to keep it running in optimal condition. If you are thinking about freeing yourself from server hassles, let's look at some of the benefits of server management outsourcing.
Happy World Password Day! The HostDime Abuse & Security team has compiled a few recommendations to share with you to help you improve your password security and keep you and your company's data secure.
Corero released a study this week polling 300 security professionals which showed that DDoS attacks can cost their organization up to $50,000 per attack, but that's not the worst part.
One of the most important decisions for an enterprise is where and how they host their operations. Unfortunately, not all data centers are created equal. The following tips should steer you the right way in choosing a reliable data center that fits your enterprises' needs.
HostDime is proud to offer enterprise-level, managed dedicated servers at our Los Angeles, California branch. The 7 story data center facility is 490,000 square feet, outside the 500 year flood plan, and is SOC2, SOC3, and HIPAA compliant.
Dropbox filed its IPO last week, and in the filing, we were able to peel back the curtain and see exactly how much they saved because of the switch from AWS to colocation data centers.
Yesterday, Github was hit by the largest DDoS attack in history. This volumetric DDoS attack was unique in achieving its massive amount of power by relying on UDP-based memcached traffic.
HostDime's fiber connection into the Orlando Gateway, one of Central Florida's largest interconnection points, will allow enterprises in 380 S. Lake Destiny to connect their most critical applications to us through this fiber via a simple cross connect and by interconnection to our nearby facility.
Bare metal servers are physical servers dedicated to a single tenant; this means one tenant has complete control, flexibility, and security over their server.


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Hurricane Matthew's forecast path has slowed and taken a significant turn to the east off the Carolinas likely saving our area from any effects of the storm, according to the latest from the National Hurricane Center.
Virtually all the weather models reflect the turn to the east once the storm moves north off the Carolina coast, which is now slated to happen Sunday morning - a full day later than previous forecasts.
Some models even point to a turn to the south once Matthew is well off the coast Monday.
Matthew remains a dangerous storm, weakened to a Category 3 after hitting Cuba. But some strengthening is expected as it takes aim at the Bahamas, then the Florida coast where hurricane warnings and watches have been posted north to the Georgia line.
On Wednesday morning, the local National Weather Service forecast dropped any reference to tropical storm conditiions for this weekend. The latest guidance on Matthew shows the storm turning out to sea Sunday.
"A cold front from our west is pushing it back out to sea," said meteorologist Brian Lasorsa of the NWS office in Sterling, Va.
"The storm was going to move up the coast before the large cold front approached, but since it has apparently slowed the front will steer it off the coast," Lasorsa said.
And that should keep any storm effects well south of our area.
Meanwhile, it looks like mostly sunny skies Thursday with a high around 70.
Friday afternoon brings a 30-percent chance of showers with temperatures holding around 70.
Saturday will be cloudy with a 60-percent likelihood of showers and slightly cooler temperatures in the upper 60s. The chance of rain diminishes to 30 percent Saturday night.
Sunny skies are in the offing Sunday through Tuesday with highs in the mid- to upper 60s.


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Study from my errors.
«Do you really want young ones? » I inquired.
«Sure we do, » he stated.
Along with that, we had been addicted. Yes, we knew which he did want kids meant overcoming the first hurdle of dating him that he was divorced and had two children, but just knowing.
But, the very first half a year, there have been much more hurdles, which resulted in the connection wearing down.
Having dated a divorced man, I would constantly wished there is some type of dating advice to simply help me personally navigate. Happily, from experience, we now know very well what to not do while the errors to prevent.
Therefore, if the man you are seeing is prepared for dating after breakup, here is tips on how to be too. Avoid these five errors whenever dating a man that is divorced.
1. You ignore their viewpoint on children.
Make certain you are both aligned from what you would like.
Also though he stated he desired young ones, i possibly could inform half means through our dating that things had changed, and I also ignored the indications. He currently had two kids and although in the first instance he stated he wished to have another beside me, we knew which he had started initially to alter their brain.
Rather, confront your situation head-on (if he has children already if you do want kids with a divorced guy), especially. To prevent adding pressure, be sure that you are both on the same path that you know.
2. You receive involved with a relationship as he's nevertheless fighting together with his ex.
Be sure he's over their ex and/or understands how exactly to manage their feelings towards her.
It felt like I happened to be in a relationship with each of these. He'd speak about her on a regular basis. This could cause lots of stress and would be transferred into then our relationship, which caused lots of anxiety on each of us. Continuar leyendo «5 Mistakes Most Ladies Make When Dating Divorced Guys»


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Today, on July the 4th, I've been thinking of dear friends and family living in America. Like Canada's national holiday a few days ago, it is a day of pride in one's nation and celebrating the vast and beautiful countries we live in. But it's also a reminder of what European settlement here has costContinue reading "American Tune"
Tag Archives: Civil Society
I Put a Spell on You
Nina Simone was unknown to me until she was the featured artist of the week on a recent episode of BBC 6 Music's Guy Garvey's Finest Hour, where he played her 1965 recording of "I Put a Spell on You." I'd likely heard other music of hers before, just didn't know who she was. I'veContinue reading "I Put a Spell on You"


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Racial discrimination is illegal in California under the U.S. constitution and Title VII of the U.S. Code. According to such laws, an employer cannot use race as the basis for making decisions regarding hiring, recruitment, wage levels, or promotion offers.
What Must Be Proven in a Discrimination Claim?
Federal investigations of workplace discrimination are done by the agency called the Equal Employment Opportunity Commission (EEOC). Employees who are victims of discrimination are required to file their claim with the EEOC before filing suit in a court of law. Racial discrimination claims involve situations where an employers treat employees differently than other employees who are of a different race.
To succeed on a claim of racial discrimination, the South Lake Tahoe plaintiff must also show that his employer acted with an intent to discriminate based on race or nationality. The employer's intent can often be shown by showing that other people of different races were treated preferentially.
How Can a South Lake Tahoe Lawyer Help with My Claim?
Also, sometimes statements that were recorded and made by the employer regarding racial issues can be used to prove intent. Depending on where you file your claim, the procedural laws will vary considerably, and so a South Lake Tahoe lawyer will help familiarize you with the requirements for your district. A good California attorney will also be able to assist you should you decide to file with the EEOC, and they can guide you towards other potential remedies while your case is pending.


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The generic and brand tend to be used for a similar purpose but they are different in where did they are taken, their dosage and every may carry their own unwanted side effects. This makes sense given that DHT, the active kind of testosterone, should be used for prostate cells and prostate type of cancer cells to cultivate. Losing hair needs time to work and struggling with that baldness-and attempting to grow it back-is a good-term effort at the same time. If its a matter of a day or two, it does not matter because people have difficulty in handling their hair and infrequently one will not be able to keep with it but if these days become weeks or god forbid, into entire time, then one should consider so that it is extremely difficult situation which will have been handled or handled immediately at the first hand only. As we discussed earlier, PSA accounts for making semen more liquid and is also a normal product from the prostate.
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Industrial doors
The industrial rolling gates constitute the basic protection of building openings of industrial houses and warehouses or commercial-service buildings from the undesirable access, burglary as well as atmospheric conditions.
Taking the investors into account, who has to confront the dilemma of the choice of appropriate gate, Aluprof is offering the industrial rolling gates.
Durability and dependability
The gate is lifted and lowered with the help of a three-phase electric servomotor 400 V AC, which guarantees its safe and long-lasting operation.
The PE 100 profile constituting the structure of the gate curtain provides stability and resistance to the mechanical influence. The profile coating is characterised by a very good stability of colour and the increased abrasion resistance, as well as it protects against the unfavourable influence of atmospheric conditions.
- the Aluprof gate can be installed both in existing objects and in the buildings under construction
- control of the gate is executed with the help of a switch located inside the hall; depending on the needs it is also possible to apply radio controllers or other preferred combinations of control devices, which ensure high comfort of operation
- the Aluprof gate operates in extreme atmospheric conditions (-25┬░C ├À 65┬░C)
- the gate can be applied to the maximum width equal to 7 m, and the height of 5 m, however the overall surface cannot exceed 31.5 m2
- the Aluprof industrial gate is also indicated by the modern appearance and a wide range of colour allowing aesthetic incorporation into the building wall
The Aluprof rolling gate is a safe solution.
In standard version, it is equipped with the following types of protection devices:
- the emergency brake constitutes protection against self-rolling of the gate curtain
- application of the contact safety sensor in the bottom safety strip constitutes the protection against crushing with the closing gate
- arrangement of photocells will react to movement in the gate light, resulting in stopping or moving backward of the gate
- the emergency manual operation enables opening or closing the gate with the help of a chain or a crank
Construction of the gate and technical parameters
The gate curtain is rolled on the steel reel of the diameter adjusted to the resistance requirements, mounted on the brackets constituting its support and bearing. The brackets are mounted directly to the wall or to other constructional elements of the object.
The servomotor transmitting power to the reel can be installed on the right or the left side of the gate. In the case of gates mounted within the lintel area, the attention should be brought to the minimum height of the lintel necessary for installation of a gate (the reeling diameter is dependent on the height of a gate ÔÇô drawing and a table).
More about industrial doors you will find at www.shutters.aluprof.eu


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