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The genus Brucella includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying Brucella to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical Brucella species or as a member of the recently identified marine mammal group.
Brucellosis is a major cause of disease in livestock worldwide, with substantial implications for animal welfare and economic output. Furthermore, as the most common zoonotic disease, it remains a significant public health concern (22). Although a few parts of the developed world have eradicated the disease by a combination of strict veterinary hygiene measures, monitoring programs, and improved food safety measures, it remains endemic in large areas. Human disease usually reflects occupational exposure or the consumption of unpasteurized dairy products and can lead to a chronic debilitating infection.
The causative organisms of brucellosis are members of the genus Brucella. Traditionally, the group is divided into six species with distinct host specificities (1), although at the genetic level, members of the genus are highly conserved (14, 32). Reflecting this, DNA hybridization experiments led to the traditional view of Brucella taxonomy being challenged and the group being described as monospecific (28). Practical considerations meant that this classification system never found widespread support, and recently, moves were made to return to the classical species designations (20). Of the currently recognized species, B. abortus is predominantly associated with bovine brucellosis, B. melitensis is predominantly associated with small ruminant brucellosis, B. canis is predominantly associated with canine brucellosis, B. ovis is predominantly associated with ram epididymitis, and B. neotomae has been reported only in the desert wood rat. The remaining species, B. suis, has a broader host range, being predominantly associated with porcine brucellosis (biovars 1, 2, and 3) but also being found in hares (biovar 2), rangifers (biovar 4), and rodents (biovar 5). More recently, novel Brucella isolates were detected in a variety of marine mammal species (7, 10, 12, 17, 23). These isolates clearly form new Brucella groups, although they have yet to receive formal taxonomic recognition (13a, 20).
Classically, Brucella isolates are divided into species (and biovars in the case of B. abortus, B. melitensis, and B. suis) by a biotyping procedure that assesses a range of cultural, phenotypic, and antigenic properties (1). While biotyping has long been the mainstay of Brucella epidemiology, there are a number of acknowledged drawbacks. As the discriminating changes between some groups can be very subtle, particularly at the biovar level, the method is rather subjective and requires substantial expertise and experience. In addition, as the method is culture dependent, in terms of both initial isolation and ongoing discriminatory tests, it is a lengthy procedure. The need for specialized and standardized reagents and containment facilities that should ideally be used for handling Brucella means that only a few laboratories in the world undertake this procedure.
Given these issues, there is a clear need for molecular tests that can rapidly and categorically identify Brucella isolates to the species level and beyond. One developing area in microbial typing and forensics is the use of single-nucleotide polymorphisms (SNPs) (5). The use of SNPs in microbial characterization has many advantages, including their unambiguous nature and the multitude of platforms that have been developed to assay such markers (24). As an example, a minimal set of six SNPs useful for dividing Mycobacterium tuberculosis into six deeply branching phylogenetic groups for use in strain differentiation was recently described (11). Other applications of SNP-based typing in diagnostic microbiology described recently include the differentiation of Burkholderia spp. (25); genotyping of Staphylococcus aureus (16, 19), Listeria monocytogenes (8), and Escherichia coli (15); identification and typing of Bacillus anthracis (9, 18, 26); differentiation of vaccine and wild-type strains of varicella-zoster virus (6); determination of Mycobacterium tuberculosis drug resistance (29, 33); and identification of members of Campylobacter jejuni clonal complexes (2).
We have recently described the population structure of the Brucella group using a multilocus sequence analysis (MLSA) approach based on nine distinct genomic fragments (32). From this work, it is clear that sequencing of these nine loci offers an excellent way of placing Brucella isolates within one of the currently recognized species. However, sequencing of nine fragments is somewhat tedious, is not always practical, and is clearly not a rapid diagnostic procedure. Nevertheless, use of the phylogenetic framework described here allowed the identification of SNPs that define distinct Brucella species. Here, we outline the development of a multiplex assay based on these "species-defining" SNPs that offers a rapid, practical, and unambiguous alternative to current approaches to identifying Brucella isolates at the species level.
Identification of SNPs.The identification of SNPs and validation of their distribution in the Brucella population have been described previously (32). SNPs used in development of the speciation assay described here are located in glk, omp25, and trpE.
PCR of target genes.PCR products representing glk, omp25, and trpE were amplified from genomic DNA or crude methanol lysates using the primers pairs listed in Table 1, as described previously (32). PCR products were purified by passage through QIAquick PCR purification columns (QIAGEN) and eluted in 50 μl of elution buffer.
SNP primer extension assay.The SNP determination assay was developed using the CEQ SNP primer extension kit (part no. 390280; Beckman Coulter) and a Beckman Coulter CEQ8800 genetic analyzer. A SNP-primer extension premix was prepared by mixing 210 μl each of 10× buffer, ddUTP, ddGTP, ddCTP, and ddATP and 105 μl of polymerase. The optimized multiplex assay was carried out as follows. The protocol uses 0.4 μl of each of the three PCR products diluted 1:2 or 1:5 in water (glk, omp25, and trpE) and 1 μl of SNP interrogation primer at 0.5 to 2.0 μM, giving a final concentration of 0.5 to 2 pmol per reaction. The SNP interrogation primers selected for the final assay are shown in Table 1. Primer concentrations used varied depending on the efficiency of each primer and were selected following preliminary work. A master mix consisting of 2 μl SNP-primer extension premix, 1 μl of primer mix (0.5 μM trp290R, 0.9 μM omp151F, 1.2 μM glk196R, 1.0 μM glk427R, 1.0 μM omp239F, 2.0 μM glk255R) was prepared and made up to 8.8 μl with deionized water. This was added to the 1.2 μl of diluted PCR products (0.4 μl × 3) to give a final volume of 10 μl. The extension reaction was carried out using the following thermal cycling conditions: an initial denaturation step at 96°C for 1 min was followed by 28 cycles of 96°C for 20 s, 64°C for 10 s, and 72°C for 30 s. Following thermal cycling, the extended product was treated with shrimp alkaline phosphatase (SAP) to remove unincorporated dideoxynucleoside triphosphates. In a separate tube, 1.5 U SAP (in 1.5 μl) and 2 μl deionized water were mixed with 1.5 μl of the primer extension reaction mix. This mix was then incubated at 37°C for 60 min, followed by 80°C for 15 min in a thermocycler.
SNP detection on the CEQ8800 genetic analyzer.In order to detect extended products on the CEQ8800 genetic analyzer, an aliquot of the cleaned reaction mixture was diluted 1:10 in deionized water. A 0.5-μl aliquot of this dilution was added to 39 μl of Beckman SLS (formamide) and 0.1 μl of CEQ DNA Size Standard Kit 80 in Beckman 96-well plates. The mixes were covered with a drop of mineral oil and immediately processed using the Beckman standard run method SNP-1 (denaturation at 90°C; injection time, 30 s; injection voltage, 2 kV; run temperature, 50°C; run current, 6.0 kV; run time, 16 min). SNP locus tags were defined on the basis of the midpoint of migration variability seen during optimization (see Table 3), and SNPs were called automatically using default SNP analysis parameters in the Beckman software (slope threshold, 10; relative peak height threshold, 10; dye mobility calibration, SNP version 1).
Identification of SNPs included in the assay.The rationale behind the assay described here was the selection of a series of species-specific SNPs that could be included in a multiplex assay facilitating the identification of an unknown Brucella isolate as a member of one of the six classically recognized Brucella species or the as-yet-unnamed marine mammal Brucella group. Species-specific SNPs were selected on the basis of sequencing of 4,396 bp corresponding to nine distinct genomic fragments from 160 strains representing all species and biovars (32). Although a number of SNPs could have been selected (see Table 4 in reference 32 for a full list), those that were found to perform best in initial optimization and included in the final assay were located in glk, trpE, and omp25. The SNPs selected are highlighted in Fig. 1 with reference to MLSA data described previously (32). The distribution and specificity of these SNPs in the Brucella population has since been further validated by sequencing of the equivalent fragments from a further 170 strains in addition to the 160 strains described previously (32) (data not shown).
The multiplex assay developed involves the interrogation of the base present at six SNP sites. Five of these have changes that, to date, are specific for a particular species or group. These markers are SNPs at position 151 of omp [omp151] for B. abortus (corresponding to omp25-3627 in reference 32), glk427 for B. ovis (glk-1557), omp239 for B. canis (omp25-3715), glk255 for B. melitensis (glk-1403), and trp290 for marine mammal Brucella (trpE-2858). The final marker, glk196 (corresponding to glk-1344 in reference 32), was originally thought to represent a change specific for B. neotomae, but this change was later found to be shared with members of B. abortus sequence type 6 (ST6). SNP markers specific for the B. suis group, as currently defined, were not identified by MLSA (32). As a result, members of this species are identified on the basis that they lack any of the species-specific markers and thus possess an overall multiplex profile that is distinct from any other currently described Brucella species. There are therefore eight predicted assay outcomes as shown in Table 2. Each species or group, with the exception of B. abortus, has a single predicted unique SNP profile. For B. abortus, there are two possible distinct profiles, labeled genotypes A and B, reflecting the sharing of an SNP between B. neotomae and B. abortus ST6 isolates. Genotype A corresponds to 107 of 110 B. abortus isolates examined to date by MLSA (our unpublished data). Genotype B is apparently much rarer in global terms, being characteristic of the B. abortus biovar 3 reference strain Tulya and a subset of African isolates belonging to ST6.
Principle of the assay.The assay described here was developed using the Beckman 8800 genetic analysis system but should be easily transferable to other fragment analysis platforms. The basis of the assay is a series of primer extension reactions. Following amplification of PCR products for the three genetic fragments of interest (omp25, glk, and trpE), there are three major steps to the assay. The first stage involves template clean-up by passage through a PCR clean-up column to remove deoxynucleoside triphosphates and primers. The second step involves single-base extension in which SNP interrogation primers anneal one base short of the target SNP and DNA polymerase inserts a dye terminator complementary to the SNP site. Finally, the extended product is treated with SAP to remove unincorporated dideoxynucleoside triphosphates before electrophoresis to identify and size the reaction products.
Assay optimization.The final assay conditions presented in this study and described in Materials and Methods were established after the systematic variation of a large number of parameters including thermal cycling conditions (annealing temperature and length and number of cycles), the amounts of premix included in reaction mixtures, primer orientation, primer concentration, and dilution factor prior to electrophoresis. SNP interrogation primers were tested initially in individual reactions to assess performance, following which polynucleotide tails were added to selected primers to give suitable separation upon electrophoresis. Tailed interrogation primers were again tested individually to assess performance and validate migration size before the six primers were tested in multiplex reactions. The performance of the assay when carrying out the six SNP reactions individually and pooling products gave results comparable to those obtained true multiplexing, where all six primer extension reactions were carried out in a single tube, indicating that multiplexing was not compromising assay performance (data not shown).
The SNP interrogation primers included in the speciation assay range in size from 21 bp to 49 bp, although migration does not exactly correspond to primer size (Table 3). SNP interrogation primers were all separated by a migration gap of at least 4 bp to ensure ease of identification. As recommended by Beckman, the annealing temperature used for the generation of the primer extension product at 64°C was selected to be at least 5°C below the lowest primer midpoint temperature (Tm). It was necessary to include a degeneracy in interrogation primer gki196R, as during assay validation, the initial primer selected failed to generate an extension product, with members of ST23 found to have a polymorphism within the selected primer binding site (glk1352). The template DNA concentration recommended by Beckman (150 fmol per reaction) was used throughout initial optimization and validation. However, in order to avoid time-consuming quantification of PCR products prior to the primer extension assay, later work was carried out omitting this step. In the final routine assay procedure, 0.4 μl of purified PCR product diluted at 1:2 and 1:5 in water was used as a template. In our hands, both these dilutions consistently give good clean results, with peaks of suitable magnitude.
Assay performance and validation.Figure 2 shows typical reaction profiles obtained with the multiplex assay demonstrating each of the eight specific profiles that the assay generates. To fully validate the assay, we examined the profiles of over 500 isolates including all species and biovar reference strains and a large number of field isolates. In all, 213 B. abortus (including 4 B. abortus isolates with the atypical genotype B profile), 111 B. melitensis, 42 B. suis, 8 B. canis, 39 B. ovis, 3 B. neotomae, and 94 marine mammal Brucella isolates were examined and found to give the predicted SNP profile. These isolates were part of a reference collection held at our laboratory that includes both historical isolates obtained from across the world over some 50 years as well as contemporary isolates coming through the laboratory as part of reference and surveillance activities. They therefore provide a robust test of assay scope. A small number of additional isolates (∼10) gave profiles that did not match the original species designation recorded in the strain archive or which appeared to represent a mixed-species signal. In all cases, examination of these isolates by full MLSA (32) and variable-number tandem-repeat typing (31) confirmed that either the original species designation was incorrect or the culture represented a mixed population.
Specificity.The currently accepted closest relatives of the Brucella group are members of the genus Ochrobactrum (27). In order to assess the specificity of the assay for Brucella, it was applied to type strains of five Ochrobactrum species (O. tritici LMG18957T, O. intermedium LMG3301T, O. gallinifaecis DSM15295T, O. anthropi LMG3331T, and O. grignonense LMG18954T). All three of the PCR products required for the SNP assay to identify isolates at the species level could be amplified from only two of these five strains (O. grignonense and O. intermedium) under the conditions examined in this study. Furthermore, when tested in the multiplex SNP interrogation assay, neither of these strains gave a complete profile. Sequencing of the PCR products allowed an examination of the regions corresponding to the interrogation primers used in the assay to identify isolates to the species level. This confirmed that there are an average of four mismatches between SNP interrogation primer sequences and the corresponding sequence in these two Ochrobactrum strains and likely explains the inability to generate a profile from these isolates. Thus, the assay appears to be specific for the Brucella group as currently defined, giving no result with the closest phylogenetic neighbors.
Conclusions.The assay described here represents a novel approach to identifying Brucella isolates to species level. SNPs are particularly attractive assay targets, as Brucella isolates represent such a genetically conserved group (13, 14, 28, 32). Reflecting this, SNPs are likely to have occurred only once in evolution and are unlikely to mutate to new states or back to their ancestral state. The assay has a number of potential advantages. It overcomes the subjectivity associated with biotyping with an approach that is technically straightforward and has substantial advantages in terms of speed. It is easily possible to complete the entire assay in a day, as opposed to the several days currently required for biotyping following isolation of a pure culture. In addition, techniques that reduce potential exposure to live organisms are desirable given that brucellosis is easily acquired by laboratory personnel in the absence of stringent biocontainment facilities. We have also shown that the technique is applicable to crude extracts of material, overcoming the need for DNA preparation as required by molecular typing methods such as amplified fragment length polymorphism analysis (30) or insertion sequence-based fingerprinting (21). Indeed, as a PCR-based method, the technique should theoretically be directly applicable to field material, obviating the need for any culture prior to typing. The principle of the assay could also be readily transferred to any of the many alternative techniques applicable to SNP determination (24). In contrast to some existing and well-established molecular methods such as AMOS PCR (3, 4) that fail to detect all species and biovars, SNP speciation is all-encompassing, identifying all biovars of all known Brucella species.
The technique also lends itself to further expansion. There is clear potential for the current scheme to identify isolates to the species level to be extended in the future to identify vaccine strains and to subtype further to the level of biovar. Furthermore, should new species of Brucella be identified, it should be possible to determine suitable species-specific SNPs based on the existing MLSA scheme and incorporate additional SNP markers into the assay, allowing the identification of these groups.
All work described in this study was funded by the VLA Seedcorn Programme (grant SC0139).
We gratefully acknowledge the assistance of the VLA Sequencing Unit, and particularly Paula Keyes, in this project; Jo Craggs of Beckman Coulter for technical advice provided in the course of this study; and James Edwards-Smallbone for assisting with Ochrobactrum work.
- Received 1 May 2007.
- Accepted 10 September 2007.
- Copyright © 2007 American Society for Microbiology


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In line 308, CGI::SSI::pm says: my $ua = LWP::UserAgent->new($ENV{HTTP_USER_AGENT} || ()); But like HTTP::Cookies, LWP::UserAgent::new takes a hash as its argument, not a list. This can be easily fixed by something like: my %conf; $conf{agent} = $ENV{HTTP_USER_AGENT} if $ENV{HTTP_USER_AGENT}; my $ua = LWP::UserAgent->new(%conf); but it is not serious because LWP::UserAgent::new has a default agent. However, as I shall argue in another RT, this is all moot, because 'include virtual' does not, and cannot, work with remote URL's, so I doubt that this code has ever been executed.


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The viral video, which was posted by SaharaReporters, shows the cleric passionately excoriating the government over its failure to make good on it's responsibilities to the Nigerian citizenry.
In the video, the cleric labeled the president as 'incompetent' and demanded his immediate resignation in view of the President's call for former President, Dr. Goodluck Jonathan to resign when the country was mired in a quagmire of insecurity during the latter's presidency.
He said: "Was it not you who told Jonathan to resign because there was insecurity in the land? Will you also not resign because of your incompetence"?
"What is our benefit as citizens? No peace, no unity. The state you are from, they are cursing you.
They are saying may Allah curse you because you are useless in their eyes".
"What is the benefit of your presidency or that you are commander-in-chief of the armed forces? May your kind be purged from the land"
"What sort of situation is this? If we talk we are arrested and detained. Anyone who attempts it, may he be cursed.
"To be honest, no one can save us from this mess if not Allah. The president is incompetent. The governors are incompetent. The lawmakers have failed. Making fake laws and deceiving the people and stealing from them".
The cleric, who spoke in his native Hausa, towards the end of his verbal tirade switched to English when he questioned, "What kind of leadership do we have in Nigeria"?
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Hayes Lawn Tennis club, [23] based at the Warman Sport ground.
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The lychgate and wall to the south are Grade II listed. Today it continues to Online gun auctions Huddersfield up with tradition, playing live music as well as offering a warm welcome to those looking for home-cooked British favourites. The only open fields shown south-west of the main road were Botwell Heath and Dawley Field.
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Find. Many field names survive from the late 14th century, fn.
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The town's name is spelt Hessee in a entry in an Inquisition post mortem held at The National Archives.


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3 edition of Food and cookery found in the catalog.
1915 by The College press in Loma Linda, Cal .
Written in English
|LC Classifications
|The Physical Object
|3 p.
|ID Numbers
|Open Library
|LC Control Number
Or simply "a bloody genius", according to Jamie Oliver. Let me persuade you, my friends, to try and persevere in adopting this very desirable kind of food, Food and cookery book in your power, for your ordinary fare. Grounded in the belief that the most nourishing foods are whole, seasonal, unprocessed, and organic, Our Journey with Food Cookery Book is a rich compilation of recipes that promote optimal health through tradition, variety and nutrient density. As participants noted, "the continued existence of many breeds This feast was organized by Bruce Kraig and prepared by Dudley Nieto. Concise sections detail most common ingredients and different cooking methods.
It is a mark of just how reliable a piece of scholarship it is that, on publication, it was greeted with almost universal acclaim; a rare achievement for any work wading into the notoriously rancorous Jewish community. But in other matters, her influence has Food and cookery book ever been benign. The published volume was edited for the first time by Richard Hosking[51] an academic who worked in Japan for many years, a specialist in Japanese food, and a regular symposiast. He had just been awarded the Erasmus Prizeand this was celebrated at a Sunday lunch in his honour. Few are as important or, frankly, as indispensable as Carrier's Great Dishes of the World, which gently explained to a Britain for whom the memories of rationing were still fresh, that there really was a world of food beyond their shores. It's perhaps debatable which is the best of these, but the one for which she will always be most celebrated is English Food.
Dad's dog-eared EP Veerasawmy paperback for his curries, indeed. The theme of the first symposium in this new location was Wild Food. Who could resist poached turbot with shrimp sauce, or a properly made Cornish pasty? International and ethnic[ edit ] Norwegian immigrant cookbook in Norwegian, published in the United States in
monetary policy of the Reserve Bank of India
Literary criticism
Embrace and Conquer
Robins New Glasses
People in pubs
Sweeping changes
Southern Ontario
The first night
Holy Russia
Linear IC devices
Living with lung cancer
WordPerfect for Windows 6.1
Statistical inference for a family of counting processes
Catering for the educationally sub-normal child
Concepts in climatology.
Many of these books, particularly those written by or for a well-established cook with a long-running TV show or popular restaurant, become part Food and cookery book extended series of books that can be released over the course of many years. Smith spoke on "False Memories: the invention of culinary fakelore and food fallacies".
Apicius, De re coquinariaan early collection of Roman recipes. Such books deal not only in recipes and techniques, but often service and kitchen workflow matters. Many contributions consisted of recipes "with comments upon their composition and their culinary possibilities", a form unsuited to reprinting in the usual volume format; some of the others were published elsewhere.
People favour risottos now, but before there was risotto, there was pilaff: buttery rice mixed with onions, garlic and tomatoes Food and cookery book have first been fried in olive oil. Unusual foods at the regular bring-your-own lunch included gravad lax and chocolate-covered garlic.
Gently grilled, or fried till crisp Food and cookery book not 'crispy', a description Elizabeth David abhorred. Hazan, knowing that some pastas are most definitely not best made at home, has made cooks everywhere feel truly proud of their jars of dried spaghetti.
Cover the dish with a crust, made with two pounds of flour and six ounces of butter, or lard, or fat dripping, and just enough water to knead it into a stiff kind of dough Food and cookery book paste, and then bake it for about an hour and a-half.
Cut the tripe up in pieces, and put it into an earthen pot, with some ale, cider, or water, enough to cover it in; add sliced onions, pepper, and salt, and a good pinch of allspice; put the lid on the pot, and set the tripe in the oven to bake for two hours.
Make one pound of flour and two ounces of dripping, or chopped suet, into a firm paste, by adding just enough water to enable you to knead the whole together. I recommend you to buy Patna rice, as it is the cheapest; it is best to soak it in water over-night, as it then requires less time to boil it, and moreover, when soaked, the rice becomes lighter, from the fact that the grains separate more readily while boiling.
As Hazan notes, the Italians like to describe such dishes as "un bocone da cardinale", or a "morsel for a cardinal". Boil the pudding four hours. Geraldene Holt spoke on "Memories of M.
I am well aware that in your homes it is not a common every-day occurrence for you to dress a large joint of meat, from which enough would be left for one or more days' dinner; but still it may, and does sometimes occur, that you have cold meat at your disposal, upon which you may exercise your knowledge in domestic economy.
When done, turn the pudding out on its dish, and, if you can afford it, pour over it the following sauce:— No. Others who addressed the symposium for the first time included Michael Abdallaa scholar of the modern Assyrians of the Near East and an Assyrian himselfthe anthropologist Jeremy MacClancythe medievalist Terence Scully and the Greek food writer Rena Salaman.
Dad's dog-eared EP Veerasawmy paperback for his curries, indeed. Specialized ateliers, basic through advanced levels Food and cookery book pastry and confectionary craft making, plated and boutique style desserts, world delicacies and precise decorating techniques.
Whether we're considering titles, Food and cookery book, or even cover design, it's clear to see things have changed in the last hundred years. Such books were written primarily for housewives and occasionally domestic servants as opposed to professional cooks, and at times books such as The Joy of Cooking USALa bonne cuisine de Madame E.
Chop up the heart, liver, lights, and the fat crow; season well with pepper, salt, allspice, thyme, sage, and shalots, and Food and cookery book this sausage-meat into balls the size of an apple, which must be each secured in shape with a piece of pig's caul fastened with a wooden twig, or skewer, and placed in rows in a tin baking-dish, to be baked for about half an hour in a brisk oven.
Pig's Fry. This precaution assists very much in rendering the meat more palatable and tender, as it is [43] by this frequent Food and cookery book over of the meat while broiling, that the juices are not allowed to run off in waste, but are re-absorbed by the meat.
Titled "Dying of Starvation in the Supermarket", her talk surveyed the problem of diminishing bio-diversity in domesticated animals and plants. In case you have no oven, send it to the baker's.
Many less-than-universal cooking media were discussed, including sheep's tail fat by Jill Tilsley Benhamcaul fat by Jane Grigson and almond milk by the medieval scholar Constance B. Acton's work was an important influence on Isabella Beeton[25] who published Mrs Beeton's Book of Household Management in 24 monthly parts between and AbeBooks has many millions of books, many of which are rare books, collectible books, antiquarian books or unique books.
Put the rice on to boil in plenty of cold water, stirring it from the bottom of the saucepan occasionally while it is boiling fast; when the grains separate at the ends, and thus appear to form the letter X, the rice will be done; it requires about half an hour's gentle boiling. Getting back to the roots of cooking with fresh ingredients and the right cookware.The bestselling author of SIMPLE, Diana Henry, rustles up a table groaning with delights, rounding up the very best in food writing and cookery books of From store-cupboard suppers and kitchen staples to revolutionary inventions, meat-free feasts and warming comfort food classics it's a selection with something to tempt everyone's tastebuds.
Gode Cookery is an award-winning medieval history website dedicated to the food & feasts of the Middle Ages & Renaissance.
Here you will find information on medieval cooking, instructions for preparing authentic feasts, hundreds of recipes, image collections, a medieval cooking discussion group, graphics, photographs, and history resources.
Online shopping for Books from a great selection of Diets & Healthy Eating, Reference & Gastronomy, Meals & Menus, National & International Cookery & more at everyday low prices.4/5.Gode Cookery is an award-winning medieval history website dedicated to the food & feasts pdf the Middle Ages & Renaissance.
Here you will find information on medieval cooking, instructions for preparing authentic feasts, hundreds of recipes, image collections, a medieval cooking discussion group, graphics, photographs, and history resources.DISHOOM: FROM BOMBAY with love is an eccentric and charming cookery book, with over recipes for the Bombay comfort food and drink that we love.
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text:
Optimal integration between host physiology and functions of the gut microbiome.
- Authors
- Type
- Published Article
- Journal
- Philosophical Transactions of The Royal Society B Biological Sciences
- Publisher
- The Royal Society
- Publication Date
- Sep 28, 2020
- Volume
- 375
- Issue
- 1808
- Pages
- 20190594–20190594
- Identifiers
- DOI: 10.1098/rstb.2019.0594
- Source
- Medline
- Keywords
- Language
- English
- License
- Unknown
Host-associated microbial communities have profound impacts on animal physiological function, especially nutrition and metabolism. The hypothesis of 'symmorphosis', which posits that the physiological systems of animals are regulated precisely to meet, but not exceed, their imposed functional demands, has been used to understand the integration of physiological systems across levels of biological organization. Although this idea has been criticized, it is recognized as having important heuristic value, even as a null hypothesis, and may, therefore, be a useful tool in understanding how hosts evolve in response to the function of their microbiota. Here, through a hologenomic lens, we discuss how the idea of symmorphosis may be applied to host-microbe interactions. Specifically, we consider scenarios in which host physiology may have evolved to collaborate with the microbiota to perform important functions, and, on the other hand, situations in which services have been completely outsourced to the microbiota, resulting in relaxed selection on host pathways. Following this theoretical discussion, we finally suggest strategies by which these currently speculative ideas may be explicitly tested to further our understanding of host evolution in response to their associated microbial communities. This article is part of the theme issue 'The role of the microbiome in host evolution'.


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An informative article, normally, is a brief written bit of writing, typically written by an individual who has either published or will be in the practice writing essays online of publishing an article that intends to provide some sort of excuse on a given subject. Essays generally often overlap with those of the article, a brief article, a leaflet, and even a brief story. Generally, essays are classified as formal and non-formal. The objective of the formal article is to present information and facts which can be used for additional research while the non-formal essay is more like a justification of a specific idea or theory, while giving an impartial point-of-view.
There are several factors that go into determining the content of a composition. Among those factors is the sort of study and facts to be presented. This includes matters such as research and facts about the subject itself, and also facts and information about the author himself. Some writers choose to write an essay on themselves, while some decide to compose an essay about their topic, even if it is a topic that is already researched by a different writer. It is up to the writer to determine what sort of essay he's going to compose.
Another factor that decides the content of this essay is that the type of writing used. Even the most common style used for essays would be the first-person point-of-view. From the first-person point-of-view, the essayist applies only the private experiences of the writer to create his arguments. Even the second-person point-of-view (or third-person point-of-view) is an alternative style essay writer into the first-person point-of-view that utilizes the writer's subjective experiences to create his arguments. At length, the constructivist style of writing is the most controversial fashion, which entails the use of various ideas and concepts as a background to create the arguments.
Because of the different points of view used in the introduction of an essay, there are various conventions used too. Generally speaking, most essays may start with an introduction, which may be either a thesis statement or even a thesis conclusion. However, these may differ based on the character and the writer.
The end, which is generally at the end of this composition, has a principal objective or result that ought to be reached at the conclusion of the essay, either an academic achievement or something else that should be concluded. These may include, although are not limited to, personal achievement, approval of a notion, arrangement on an end or view, the decision that is a compromise of one or more of the thesis, conclusion that shows that an individual or team has failed in its own attempts, decision that finishes that you did something wrong, conclusion that shows an individual or group has accomplished something, conclusion that shows a pattern of failure previous attempts, etc..
Writing a composition could be a little more challenging than what you could think, but it can also be a lot more rewarding. If you are having trouble deciding what sort of article to write, take some time to find out exactly what your kind of writing is and then figure out ways to incorporate that in your writing style. Keep in mind an essay will have a target, but that objective doesn't always have to be fulfilled, just it does need to create an impact in your reader's mind and purpose to the reader's interest.


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The five lines of the poem Kubla Khan sound like a chant or incantation, and help suggest mystery and supernatural themes of the poem. Originally, his dream included between 200 and 300 lines, but he was only able to compose the first 30 before he was interrupted. Water imagery permeated through many of his poems, and the coast that he witnessed on his journey to Linton appears in Osorio. Like Xanadu, art offers a refuge from the chaos. Coleridge claims that while he slept, he had a fantastic vision and composed everything in a flash, while sleeping— some two or three hundred lines of poetry. It was his own poem, a manifesto.
Allegedly written after a laudanum an opiate induced dream, the author claims to have been planning a two hundred to three hundred line poem before he got interrupted by a 'man from Porlock,' after which he had forgotten nearly all of his dream. They are cut across at each knot, and then the pieces are split so as to form from each two hollow tiles, and with these the house is roofed; only every such tile of cane has to be nailed down to prevent the wind from lifting it. Fountains are often symbolic of the inception of life, and in this case may represent forceful creativity. Cambridge: Cambridge University Press, 2002. Coleridge of a reverend friend of ours, who actually wrote down two sermons on a passage in the Apocalypse, from the recollection of the spontaneous exercise of his faculties in sleep. He says that once in his dream he saw a girl who was brought from Abyssinia. Through the use of vivid imagery Coleridge reproduces a paradise-like vision of the landscape and kingdom created by Kubla Khan.
In these lines, he says that if he could recall or learn the ravishing music of the Abyssinian girl, he would build the beautiful palace of Kubla Khan in air. By what process of consciousness could he distinguish between such composition and such reminiscence? After his father died in 1781, Coleridge attended Christ's Hospital School in London, where he met lifelong friend Charles Lamb. It is possibly half-inherent in his subject. While there he mastered the German language and began translating. In the poem Kubla Khan by Samuel Coleridge, language is used to convey images from Coleridge's imagination.
Coleridge, whose early work was celebratory and conventional, began writing in a more natural style. A copy of the manuscript is a permanent exhibit at the in London. A single verse is not poetry unless it is a one-verse poem; and even the finest line draws its life from its context. There are images of paradise throughout the poem that are combined with references to darker, more evil places. Coleridge: The Critical Heritage 1834—1900. The poem also contains allusions to the Book of Revelation in its description of New Jerusalem and to the paradise of William Shakespeare's A Midsummer Night's Dream.
Kubla Khan In Xanadu did Kubla Khan A stately pleasure-dome decree: Where Alph, the sacred river, ran Through caverns measureless to man Down to a sunless sea. This would have allowed Coleridge to purposefully write the poem as a fragment. Some people think that Coleridge just made the whole thing up. Opium was for him what wandering and moral tale-telling became for the Mariner — the personal shape of repetition compulsion. According to the directions of his Astrologers or Magicians, he on the eight and twentieth day of August aforesaid, spendeth and poureth forth with his owne hands the milke of these Mares in the aire, and on the earth, to give drink to the spirits and Idols which they worship, that they may preserve the men, women, beasts, birds, corne, and other things growing on the earth.
It seemed an enchanted place haunted by demons and fairiers and frequented by a disappointed lady-love weeping for her demon-lover under the light of the fading moon. Overview According to Coleridge's Preface to Kubla Khan, the poem was composed one night in an opium influenced dream after reading a work describing the king. The Creative Mind in Coleridge's Poetry. The construction of the Palace is so devised that it can be taken down and put up again with great celerity; and it can all be taken to pieces and removed whithersoever the Emperor may command. There are images of two women in the poem and they are a direct contrast to each other, one representing evil, and the Abyssinian maid exotic and beautiful. They might even quote the first few lines. In the pleasure-house Kubla Khan became addicted to luxury so his ancestors urged him to shake off his lethargic and luxurious life and be ready to life of adventures and wars.
Even when we make all due allowance for the prejudices of critics whose only possible enthusiasm went out to 'the pointed and fine propriety of Poe,' we can hardly believe that the exquisite art which is among the most valued on our possessions could encounter so much garrulous abuse without the criminal intervention of personal malignancy. The effect could scarcely have been more satisfactory to the ear had every syllable been selected merely for the sake of its sound. Coleridge spent most of the trip in Germany, studying the philosophy of Immanuel Kant, Jakob Boehme, and G. Coleridge, when composing the poem, believed in a connection between nature and the divine but believed that the only dome that should serve as the top of a temple was the sky. The Archaeological Site of Xanadu The archaeological site of Xanadu. I remember the other's coming away from him, highly struck with his poem, and saying how wonderfully he talked.
The work was set aside until 1815 when Coleridge compiled manuscripts of his poems for a collection titled Sibylline Leaves. London: Jackson and Walford, 1847. One of the most known warriors, who had taken almost the entire world, is Genghis khan. During his time of leadership he quickly Composition and Publication History of Samuel T. There also is strong a break following line 36 in the poem that provides for a second stanza, and there is a transition in narration from a third person narration about Kubla Khan into the poet discussing his role as a poet. His flashing eyes, his floating hair! In his vision he saw an Abyssinian maid playing upon her dulcimer. The dream like quality of the poem makes it even more cherishable.
Of these ideas, Coleridge's emphasised the vastness of the universe and his feeling overwhelmed by how little the universe seemed to him. Xanadu symbolises the fabled Garden of Eden, it is lovely and innocent, surrounded by evil and the constant threat of destruction. A contract was drawn up on 12 April 1816 for 80 pounds. In the poem Kubla Khan, Coleridge uses contrasts in the images he presents to his audience. Influenced by Plato's Republic, they constructed a vision of pantisocracy equal government by all , which involved emigrating to the New World with ten other families to set up a commune on the banks of the Susquehanna River in Pennsylvania.


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Managing Orthopaedics at Rittenhouse Medical Center
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June 01, 2007
Harvard Business School
Considers the issues associated with running multiple business models–a private practice and an academic faculty practice–within the confines of the orthopaedics department of a single medical center. Students assume the role of Neela Wilson, Executive Director of Rittenhouse Medical Center, in managing the operational requirements of, and organizational tensions created by, these competing models. In analyzing the case, students have the opportunity to: (1) gain a better understanding of operational focus and the concept of a "focused factory" in health care, (2) consider the concept of a "factory within a factory" in the context of an academic medical center, and (3) build an appreciation of the managerial challenges associated with operating related, and often competing, business units within the same organization.
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Managing Orthopaedics at Rittenhouse Medical Center
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- Wed Mar 19, 2014 00:12
- Forum: Hyde Park
- Topic: Australian And New Zealand Resources Discusion
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Hi Oneils Thanks for the welcome. I'm in Seaspray, (near Sale). Its great to know there are a few more sausage makers in the area. The dried blood got here a week and a half after ordering. I have ordered more so should be fairly right for now. 1kg makes 6 litres of blood. Thanks very much for the o...
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- Mon Mar 10, 2014 02:35
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Hi folks after looking every where for a supplier of pork blood either dried or fresh i found out it was illegal to buy from the abattoir or butchers here in Australia. After looking further i found a store on amazon.co.uk that sells dried blood for black pudding and they do send to Australia but we...
- Mon Mar 10, 2014 02:25
- Forum: Recipes from around the world
- Topic: Biltong making - for Crusty044
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I havent tried cold smoking my biltong yet but will as soon as i make a smoke generator. unfortunately i only have a masterbuilt hot smoker at the moment but am in the process of buying the stuff needed for a cold smoker. When im in Melbourne i try to get to the Victoria Market because there is a li...
- Sun Mar 09, 2014 13:53
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- Topic: Biltong making - for Crusty044
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Hi All, I've been making Biltong for several years now and have never used a cure besides salt. I usually make it the way Janlab does however the last few times all I've used is equal parts of dark soy sauce and Worcestershire sauce with a few teaspoons of crushed garlic and chili for around 2 kg of...
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PG | | Comedy, Musical, Romance
A repressed, middle-aged divorced U.S. Greek meets a young singer through a dating service and becomes smitten.
The role of Sheila Shea was originally written for Sandy Dennis. Paul Dooley was seriously allergic to cats though, and when cat-lover Dennis would come to the script readings with up to five cats at a time, he was briefly hospitalized. The role was then offered to Shelley Duvall, who turned it down. As a result, 'Allan Nichols' re-wrote the role of Sheila Shea from an Earth Mother type to the young singer/groupie played by Marta Heflin. Both stars had appeared in the director's previous film, "A Wedding."
ER doctor:
You've got to stay in bed for a while. Do you want somme pain killers?
Sheila Shea: Yes.
Alex Theodopoulos: No!
ER doctor: Some doctors don't like to give out painkillers, but when you've seen as much pain as I have, it makes you want to kill it.
ER doctor: I don't think you two should be kissing while I'm...
$1,500,000 (estimated)
As Will Smith joins the cast of "The Fresh Prince of Bel-Air" for a reunion on HBO Max, we take a look at his rise to fame.


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Bit expensive but worth it
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I use this moisturizer in the summer when my skin is feeling tight but I don't want a heavier lotion, it feels heavenly as it's so light and yet it leaves my skin looking plumper and brighter!
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Very light
I ran out of moisturizer, but I have quite a few samples, so I tried this Clinique one. It was very lightweight, absorbed quickly, the scent is light and pleasant. My face stayed moisturized, felt like I had nothing on.
These reviews are the subjective opinions of ChickAdvisor members and not of ChickAdvisor Inc.