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The overall aim is to establish a 3D retinal cell model which is physiologically-competent and predictive of human physiology. The model should consist of all the major cell types of the retina and enable their interplay: Müller and microglia, RPE and neurons (including photoreceptors). The model needs to resemble key morphological and functional features which can easily be addressed with a panel of functional readouts.
Over 60 million people worldwide are blind (Zhang K. et al., 2012). One of the leading causes of blindness in the ageing population is Age Related Macular Degeneration. There is currently no cure for this debilitating condition.
The eye is a complex organ comprising three major structures: the cornea, the lens and the retina. For the cornea and to a minor extent for the lens, in vitro models are available that enable, for example, testing of compounds for their potential to induce corneal irritation.
For the retina, there are no adequate in vitro models mainly due to its complex structure which consists of multiple cells types, including glia, neurons and the retinal pigment epithelium cells (RPE). There are some simple preclinical models available - for example, retinal explant organotypic cultures from early postnatal rodents resemble the retinal morphology to a high degree and contain the relevant cell types including Müller cells and RPE. However, they do not fully mature when cultured in vitro and therefore their translation to the mature human retina is limited. Human tissue is not readily available (Caffe A.R. et al., 2000, Pinzón-Duarte G et al., 2002) for these studies. Human-based in vitro models using primary or cell lines such as the RPE cell line ARPE19 (Dunn K.C. et al.,1996; Seigel G.M. et al.,1999) and the Müller cell line (MIO-M1) (Limb G.A. et al., 2002) do provide useful tools but again, their relevance to the mature human retina is limited.
Complex human stem cell-derived models (Gonzalez-Cordero A. et al., 2013) are beginning to emerge which form retinal spheroids in vitro but these resemble embryonic retinal development whereas mature phenotypes for example a ganglion cell can take up to 30 days to reach maturity. The spheroids often lack Müller and RPE cells which are key to a fully predictive model. Throughput is another concern as the generation and maintenance of these spheroids is often not amenable to industry use.
In summary, the currently available in vitro models have a number of limitations:
- They are typically based on a single cell type in culture and do not reflect the architecture of the mature human retina and the complex interplay between multiple cell types (e.g. photoreceptors, glial cells, endothelial cells, RPE cells).
- Integrated and functional measurements such as electrophysiology and high content imaging other than single cell behavior cannot typically be performed.
- Organotypic cultures of retina explants are typically not stable beyond a maximum of two to three weeks.
As a result of the current limitations, most pharmacological and toxicological studies to assess effects of drug candidates on the retina need to be performed in vivo. The degree of visual acuity and structure of the retina differs between animals and humans, reflecting the distinctive role of visual function between species (e.g. rats have more rods for low light scotopic vision, whilst humans have more cones for bright light phototopic vision, Jacobs et al., 2001). For models more representative of human visual function, it would therefore be highly desirable to have access to a human-relevant retinal cell model to support compound testing in vitro.
Many other retinal diseases have a genetic background and are lacking suitable animal models. Utilising patient-derived iPS retinal cell models could integrate a disease background and phenotype for compound testing which would address these concerns and provide the opportunity for the development of novel safety testing strategies.
Recent advances in the field of tissue engineering, such as by bioprinting, now provide the capabiity to address the development of a 3D model comprising the three major retinal cell types: Müller and microglia, neurons and RPE. This is the aim of this CRACK IT Challenge.
Such a model would enable the in vitro assessment of drug candidate-induced alterations on the cellular phenotypes and functions which have relevance in vivo, and which can be used not only for pharmacological and early toxicological assessment but also for disease modelling. This would also provide the potential for a leaner early drug development programme for ophthalmology indications as, for example, optimisation related to mechanistic toxicity could be performed earlier in the cycle, shortening preclinical development and enabling faster time to the clinic.
3Rs benefits
Worldwide, there are currently more than 600 R&D projects in the field of ophthalmology and the market for therapies targeting retinal disorders is expected to grow to $14.8 billion by 2022 (Pharmaventures, 2015). The majority of studies for efficacy and safety testing in opthalmological drug development are performed in animals (mainly rodents and rabbits) that provide both functional and histological readouts. For example, in vivo studies investigating ocular safety of new compounds use at least 20 animals per compound.
The proposed new model set by the Retinal 3D Challenge has the potential to replace the use of animals in the discovery of new opthalmologic drugs as well as screen out a significant number of compounds that would fail later in the pipeline, thus avoiding unnecessary animal use. The in vivo studies that are still required will be designed with improved mechanistic toxicological and pharmacological knowledge to enable more relevant dosing and group numbers and potentially deliver more refined humane endpoints.
Phase 1 winners
- Professor Michael Cheetham, University College London, £99,999.
- Professor Stefan Liebau, University of Tübingen, £99,982.
- Professor Majlinda Lako, Newcastle University, £99,982.
Phase 2 winner
Project team lead by:
- Professor Lyle Armstrong, Newcells Biotech Ltd £1,000,000.
Full Challenge information
Assesment information
The following Challenge Panel considered applications submitted to this Challenge:
|Member Name
|Dr Malcolm Skingle (Chair)
|Dr Philip Hewitt (Sponsor)
|Merck KGaA
|Dr Michael Schmitt (Sponsor)
|Merck KGaA
|Dr Stefan Kustermann (Sponsor)
|F. Hoffmann-La Roche Ltd
|Dr Marianne Uteng (Sponsor)
|Professor Mark Hankins
|University of Oxford
|Dr Julie Sanderson
|University of East Anglia
|Professor Julie Daniels
|University College London
|Dr Francois Paquet-Durand
|University of Tübingen
|Professor Rachel Williams
|University of Liverpool
|Dr Martino Picardo
|Stevenage Bioscience Catalyst
Budget information


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By Andrew M. Peterson,William N. Kelly
Read or Download Managing Pharmacy Practice: Principles, Strategies, and Systems (Pharmacy Education Series) PDF
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Managing Pharmacy Practice: Principles, Strategies, and Systems (Pharmacy Education Series) by Andrew M. Peterson,William N. Kelly
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With Steve Gosling & Mark Banks
Join Steve Gosling and Mark Banks on this 5 day practical photography workshop exploring the spectacular Northumberland Coast with its sweeping sands, castles and harbours.
5 Day Workshop - 29th April to 3rd May 2019
We will be based at the Dunstanburgh Castle Hotel in the lovely village of Embleton. Although accommodation isn't provided as part of the workshop (we like to give people the flexibility to make their own arrangements according to their taste and budget) we do encourage participants to stay in the vicinity of our base.
Over the five days we will explore this wonderful part of Northumberland, with its dramatic coastline, sandy beaches and castles in stunning settings that offer the landscape photographer image making heaven. This workshop will take you to the coast around Dunstanburgh Castle, the sweeping sands of Embleton Bay, the spectacular and impressive Bamburgh Bay (with its theatrical castle sat on a rocky perch overlooking the sea), the working fishing port at Seahouses and to magical Holy Island with its castle, priory and harbour.
We will also offer an opportunity for you to take part in a Portfolio Review session where you can bring along some of your images for constructive comment and critique. In addition, participants are encouraged to bring along their laptop computers for advice on post-production tips and techniques.
There will be a minimum of 6 and a maximum of 8 participants on the workshop.
This workshop is open to all photographers
Whats Included
Tuition from two experienced landscape photographers.
Transport to photographic locations for the duration of the workshop.
Whats Excluded
Personal Insurance
Food & Drink
Further Information
Please Note: All workshops must be financially viable for us. If we get less than 6 participants then we may decide to run the workshop with just one instructor (in this case Steve Gosling). We also reserve the right to cancel a workshop in the event of insufficient bookings and we will do this no later than 4 weeks of the due date if adequate spaces are not filled. We strongly advise you ask where you are staying, what their cancellation policy is in case of such a cancellation as we cannot and will not be held responsible for any cancellation fees charged to you.
Cancellations will be accepted if the request is received at least 10 weeks before the workshop start date but an admin fee of £100 will be retained from the deposit. No refunds will be possible for cancellations after this date unless we are able to fill the space on the workshop
About Mark Banks
A Licentiate of the Royal Photographic Society, Mark has been making images for many years and has a deep passion for landscape photography. Mark uses both film and digital cameras and specialises in teaching post-production techniques including Adobe Lightroom and Photoshop on a one-to-one or group basis.


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Musiclab RealStrat Electric Rhythm Guitar Accompaniment [Download]12-41275
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RealStrat — sample-based virtual instrument with an innovative approach to guitar sound modeling and guitar techniques emulating on a standard MIDI keyboard.
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Belfast Film Festival 2019 picks
Ten straight days of cinematic wonders await as Belfast Film Festival runs from 11th-20th April 2019. This years festival sees 90 features and 80 shorts being screened with 34 different countries being represented. Add into this Belfast Film Festival's continued commitment to the F-Rating and I'm convinced (and mildly biased) in declaring my home city's right to hosting one of the most diverse and eclectic festivals around.
There is so much to see and the program bulges with big films with star names, independent films from Northern Ireland and some of films weirder eccentricities via the Twisted Cornea and Altered States sections of the programme. There are also guests a plenty with Robert Carlyle, Lenny Abrahamson and Bollywood icon Amir Khan all set to attend.
So after a long intensive read through the programme, elimination of potential clashes, a film festival pass purchase and a schedule in place; these are a few of my picks and where you will find me over the festival period.
A Bump Along The Way
Written by Tess MCGowan, directed by Shelly Love and produced by Louise Gallagher, the film will open this years festival and centres around the strained relationship between immature mum Pamela and disapproving daughter Allegra.
When Pamela become pregnant following a drunken one-night stand the relationship between mother and daughter is put to the test.
A Bump Along The Way plays at Movie House, Dublin Road on Thursday 11th April at 7pm.
Eighth Grade
Thirteen year old Kayla endures the tidal wave of contemporary suburban adolescence as she makes her way through the last week of middle school - the end of her thus far disastrous eighth grade year - before she begins high school.
Habving heard great things about this film from reviewers and critics in the States, this was always going to been of my picks!
I'm expecting coming of age in the digital era here with a more tech savvy, social media awareness than those that occupied the spaces of Clueless and Mean Girls.
Eighth Grade plays at Movie House, Dublin Road on Wednesday 17th April at 7pm.
The Salvation Door
A seventy-year-old man, a British Indian lives in plight after being abandoned by his family, except for his married daughter called Udita, who nurtures him.
When his present state of living changes, he decides to go to "The Salvation Door" in India.
The film follows the twist and turns of his journey and numerous choices he must make, will he be able to pick his "Last drop of his life"?
Showing as part of the NI Independent section of the programme a title like this was always going to intrigue and attract me. How could Films andFaith resist?
The Salvation Door shows at Movie House, Dublin Road on Sunday 14th April at 1pm.
Animal Kingdom
Animal Kingdom depicts a man's voyage into the deep recesses of the very film that projects him, affecting its construction, progression and physical stability. While stranded in a large forest where day and night interchange at any moment, he discovers two natives who have begun their transformation into animals.
Even with that description in the festival programme, I'm still not sure what to expect here. However when a films described as a spiritual odyssey I was always going to be intrigued and compelled to check it out.
Animal Kingdom plays at QFT on Sunday 14th April at 3pm.
Dead Good
In Brighton, a small group of women have been changing the way people look after their dead. Supporting them during the ritual of care after death, and empowering them to create personal ceremonies reflecting the life that the deceased lived.
Relatively unknown, there is an emerging movement changing the way death is approached in contemproary Britain.
Death is never easy to talk about, look at or contemplate. A documentary that may look at how we challenge those ideas jumped out from the programme as something that will no doubt give me something to chew over and wrestle with.
There will be a panel discussion, featuring director Rehanna Rose, following the film and these panels always add to the experience as you learn why the film was made, the thought process and the impact it had on those involved.
Dead Good plays at QFT on Saturday 20th April at 3pm.
These are just a few quick picks from what is an extensive programme. There are many more great films, events, talks and experiences to be had across the festival period (11th-20th April).
So grab a programme, circle your picks and I will hopefully see you there.


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People seeking to buy or sell homers are required to take more caution so they aren't exploited. Recently, dealing with arrowhead homes for sale and buyers have become a point of concern. However, if you are well prepared and you want to dispose of your house, you can get a legitimate deal. There are many people that seek to have their houses bought for cash in an instant manner. They face more hardships since the economy is letting them down. There are fewer buyers that show interests with homes. Many people value renting homes rather than buying them. There is a percentage of people that put more interests in home buying. Reaching them requires you to devise many tricks that will entice and appeal to them to buy your home. There are superb ways you can do to make your home more exquisite and valuable for sale. Research about these aspects and you will discover that these ways are used by experts. Make your house ready for sale by doing the following things.
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It's been a minute, but after a long and restful summer of travel and family I'm back in the record room and looking forward to what is shaping up to be quite a club season. The first LOVE CALL party isn't for another month yet, and you'll have to wait a bit longer for another project I'm starting with Nicson from Flumo Recordings, but I'll be making a few appearances before that (in Seville and Cordoba). The first is with beGun, Reyes Estrada, and Esteban Bove from LIE on day one of Sala Malandar's big OPENING PARTY on Friday, September 7. I'll be playing peaktime, so bring those dancing shoes. For more info click HERE.
Tag Archives: House
LOVE CALL & LIE RADIO present ON AIR
The people have spoken, and we have responded. A daytime, outdoor ☀LOVE CALL☀ is finally in the works with our friends at ☀LIE RADIO☀. Join us for a nice long afternoon of music, food, fun, friends and family–oh, and the the entry is free!
Alphone [Industrias94]
Andrew Oddio [Music People – Love Call]
Esteban Bove [LIE RADIO]
Jade Tansa [LIE RADIO]
Lost Twin [Spaced Out Family]
Samuel Baron [Love Call]
☀"Gri Gri" Taller de Danza de África del Oeste – West African Dance Workshop con Silvia Roldán y música a cargo de Chardin Petit Lopez/ West African dance workshop
☀Taller de pompas gigantes / Giant bubble workshop
☀Animación infantil / Activities for kids
Love Call Guest Mix by JSD (Groove Believers, BCN)
Our good friend and collaborator Jordi Sagrera will be paying Love Call a visit this month, and was nice enough to play an exclusive set for us. He's captured the feel of our parties rather well for never having been to one. We're very excited to hear more on March 17.


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turning 90 and surrounded by love
I met this woman years ago at a Seadogs baseball game that was also a fundraising event for ALS. From the first time we said hello, I really really liked her. You know how that happens … that instant connection and that instant fondness. I photographed a family wedding for her not too long after we met, and as the years went by, every now and again we'd touch base. This last time we communicated it was about photographing her and her extended family in celebration of her sweet mother's 90th birthday. 90!! How wonderful is that??? The party would be held on the fourth of July in her mom's backyard.
It was a delight to spend an hour with these folks and be a part of a very special birthday for a very lovely lady.
The advantage of being 90 is that there are a lot of people to love.
Jane is a family and event photographer covering maine, new england and beyond.
Contact Jane to schedule your family session.


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Unpaid public sector workers can now buy goods at the various markets across the country using government assurances, popularly known as Mahama Promises.
Analysts at the Ghana Stock Exchange report that the new currency, Mahama Promise, has a higher value than the Ghana cedi, and shows remarkable robustness against foreign currencies like the US dollar and the UK pound sterling.
Highly encouraging reports from selected markets in Accra indicate that one unit of Mahama Promise can purchase two bowls of tomatoes, a bowl of garden eggs, two bunches of bananas, lots of pepper, many pieces of Maggi cubes, four gallons of Frytol, eight pieces of yam, lots of cassava, plenty of fish, some meat, one piece of made-in-Ghana wax print, and a year-long uninterrupted power supply.
The Minister of Finance, Honourable Seth Tekper, has praised the president for coming up with this home-grown solution to the salary arrears of public workers. 'This will ensure that public sector workers will not feel stranded until next two years when their salaries will be finally ready,' he told our correspondents.
Meanwhile, the troubles on the labour front just took a turn for the outlandish after one of the striking doctors, Dr Jones Gyedu, issued a bizarre threat to government communicator, Sam George. This comes after Sam George had accused the doctor of spanking the monkey in public.
The doctor made a solemn promise to inject Sam George with the drug Quinolones next time he visits a health centre: 'I have diagnosed this man with coccidiosis,' Dr Gyedu said, 'and it is my patriotic duty to cure him of it.'
Facebook users will be aware that this is not the first time Sam George has taken to the social media site to insult persons who have disagreements with his party. And his superiors in the Flagstaff House do not want this to be his last time.
Editor's note: The 'Inside the News by Mpakoo' column which appears every Monday exclusively on ghanabusinessnews.com is satire.


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- published: 14 Jan 2015
- views: 135607
Grease: The Original Soundtrack from the Motion Picture is the original motion picture soundtrack for the 1978 film Grease. It was originally released by RSO Records. The song "You're the One That I Want" was a US and UK #1 for stars John Travolta and Olivia Newton-John. It is the second best-selling album ever released and top-selling soundtrack in history with 44.7 million copies sold worldwide.
Besides performers John Travolta and Olivia Newton-John, the album also featured songs by rock n roll revival group Sha Na Na as well as the hit song "Grease", a tune written by Barry Gibb (of The Bee Gees) and sung by Frankie Valli (of The Four Seasons) that was an additional U.S. number one.
The soundtrack was released in April 14, 1978, two months ahead of the film's release. The cover gives credit to, and prominently features, the two stars of the film—John Travolta and Olivia Newton-John—although they only appear on seven of the 24 tracks. The remainder of the album is sung by various cast members and Sha Na Na, a group who performed many of the 1950s numbers in the film. The title track was recorded by Frankie Valli, who had no other connection with the film.
Grease is a 1971 musical by Jim Jacobs and Warren Casey with additional songs written by John Farrar. Named after the 1950s United States working-class youth subculture known as greasers, the musical is set in 1959 at fictional Rydell High School (based on Chicago, Illinois' William Howard Taft School) and follows ten working-class teenagers as they navigate the complexities of peer pressure, politics, personal core values, and love. The score attempts to recreate the sounds of early rock and roll. In its original production in Chicago, Grease was a raunchy, raw, aggressive, vulgar show. Subsequent productions sanitized it and tamed it down. The show mentions social issues such as teenage pregnancy, peer pressure and gang violence; its themes include love, friendship, teenage rebellion, sexual exploration during adolescence, and, to some extent, class consciousness/class conflict.
Grease was first performed in 1971 in the original Kingston Mines Chicago club, located in an old trolley barn (now the site of a hospital parking garage). From there, it has been successful on both stage and screen, but the content has been diluted and its teenage characters have become less Chicago habitués and more generic. At the time that it closed in 1980, Grease's 3,388-performance run was the longest yet in Broadway history, although it was surpassed by A Chorus Line a few years later. It went on to become a West End hit, a hugely successful film, two popular Broadway revivals in 1994 and 2007, and a staple of regional theatre, summer stock, community theatre, and high school and middle school drama groups. It remains Broadway's 15th longest-running show. Aspects of the stage play would be incorporated into the production's 2016 live TV musical.
Coconut is the third full-length album by London-based band Archie Bronson Outfit and was released on 1 March 2010.
"Shark's Tooth" was the first single from the album and was released on 22 February 2010. Initial copies of Coconut featured an accompanying DVD of videos for each track on the album.
Blue Magic can refer to:
Blue Magic is an American R&B/soul music group, and one of the most popular Philadelphia soul groups of the 1970s. Founded in 1972, the group's original members included lead singer Ted Mills with Vernon Sawyer, Wendell Sawyer, Keith Beaton, and Richard Pratt. Their most notable songs included smooth soul ballads such as "Sideshow", "Spell", "What's Come Over Me", "Three Ring Circus" and "Stop to Start."
Blue Magic was formed in Philadelphia, Pennsylvania in 1972 when former member of The Delfonics Randy Cain brought in singer-songwriter Ted Mills to do some writing with the Philly-based WMOT production company to create a new band. A short time later the group Shades of Love, featuring Keith Beaton, Richard Pratt, Vernon Sawyer and his brother Wendell, came in to audition. (According to Marc Taylor in his book 'A Touch of Classic Soul of the Early 1970s',[1] "although the group performed admirably, they lacked a standout lead singer".) The execs decided to replace the Toppicks, the act Mills recorded with. They inserted Shades of Love (which they owned contractually) with Ted Mills and retitled the group Blue Magic. They were signed with Atco Records through WMOT in the same year.
"Blue Magic" is the first single from Jay-Z's tenth studio album, American Gangster., the song was released as a single on September 20, 2007. In the chorus Pharrell sings an interpretation of the song "Hold On" by R&B girl group En Vogue, who are also featured in the song. The name "Blue Magic" is a reference to a potent form of heroin sold by Frank Lucas at the height of his drug trade in Harlem, New York. An official remix features Pharrell and Trey Songz.
The lyrics in Jay-Z's first verse are a reference to Eric B. & Rakim's song "My Melody." Most of the song itself is a reference to the 1980s. Jay-Z even brings up his stint as an early age drug dealer with links to the Iran-Contra scandal and CIA and Contras cocaine trafficking in the US that rocked the 1980s and Reagan Presidency.
Blame Reagan for making me into a monster
Blame Oliver North and Iran-Contra
I ran contraband that they sponsored
Before this rhyming stuff we was in concert
Blue Magic also samples the 1931 horror film, Frankenstein.
Coconut oil, or copra oil, is an edible oil extracted from the kernel or meat of mature coconuts harvested from the coconut palm (Cocos nucifera). It has various applications. Because of its high saturated fat content, it is slow to oxidize and, thus, resistant to rancidification, lasting up to six months at 24 °C (75 °F) without spoiling.
Numerous health authorities advise against regular consumption of coconut oil due to its high levels of saturated fat (similar to that of animal fat) having potential to increase risk of cardiovascular disease.
Coconut oil can be extracted through "dry" or "wet" processing. Dry processing requires that the meat be extracted from the shell and dried using fire, sunlight, or kilns to create copra. The copra is pressed or dissolved with solvents, producing the coconut oil and a high-protein, high-fiber mash. The mash is of poor quality for human consumption and is instead fed to ruminants; there is no process to extract protein from the mash. A portion of the oil extracted from copra is lost to the process of extraction.
I remember my mother using these products on my hair as a child, which made styling much easier on my hair and hers. I have Alopecia hair loss, but for the past year my hair has seen growth I have been using the Blue Magic coconut oil this product is great for natural hair girls like me. Alopica update: https://youtu.be/VrC5w0nsRxc J. Strickland & Co http://www.jstrickland.net/ Coping with Alopecia https://www.youtube.com/playlist?list=PL98F4EFB0B98785E2 J. Strickland Welcome: http://www.jstrickland.net/welcome.html Blue magic hair care line: http://www.bluemagichaircare.com/
Hey guys! I'm using hair grease on my natural hair for the first time in years. I will be doing a wash n go using hair grease. Also I share my thoughts on using hair grease on natural hair. Does It Work? Watch to find out the results of using hair grease on natural hair. I apologize about the difficulties at the end of the video. Hope you guys enjoy! Like, Comment, & Subscribe Products: Softee Coconut Oil Hair Grease: http://amzn.to/2iKcZsb Softee Mango Butter Hair Grease: http://amzn.to/2x8OoDg Follow me on Instargram @ queentee36 Business Inquiries: firstname.lastname@example.com
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Coconut oil has many health benefits that are well-documented. However, one recently discovered benefit is that coconut oil can help treat symptoms of hypothyroidism, or an under-active thyroid. Hypothyroidism occurs when the body's thyroid gland does not make enough hormones to allow the body to function appropriately. Coconut oil contains a fatty acid called lauric acid, which has anti-inflammatory properties. Coconut oil also contains capric acid, which has antimicrobial and antiviral properties. It is recommended that you use extra virgin coconut oil for the best health benefits. One tablespoon of extra virgin coconut oil should be consumed daily. It can be added to hot beverages, such as tea or coffee, and added to daily cooking, drizzled over a salad or added to a smoothie. Today...
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In this video, I'll be showing and discussing why I decided to start sealing moisture into my natural hair using Blue Magic hair grease. I started sealing my hair with grease on October 8th and so far, so good. My hair is able to hold on to moisture longer throughout the week without me having to re moisturize. This is a method that I plan on trying throughout the colder months in hopes of continuing to retain moisture and length over time. PRODUCTS USED: ORS Olive Oil Aloe Shampoo African Pride Moisture Miracle Detox Mask Bella Curls Coconut Whipped Leave-in Blue Magic Indian Hemp grease #NaturalHair #Type4Hair #MoistureRetention
So back in April/May 2016 I started noticing my daughter's hair started falling out more in one spot. She actually had little small patches throughout her head but they went away on their own. The doctor said it was normal for kids to experience this type of hair fall. But there was one spot that just became bigger and bigger. I tried all kinds of oils (coconut, jojoba, jbco, tea tree etc) on the spot and still nothing. Then I decided to try old fashion hair grease ($1 at the dollar store) and that was my saving grace. I know hair grease isn't the natural way but sometimes the natural way isn't always the right way. This works for her. The spot still hasn't fully grown back yet but it's definitely not where it was last year. I will continue to apply the grease as needed which right now is ...
Grease: The Original Soundtrack from the Motion Picture is the original motion picture soundtrack for the 1978 film Grease. It was originally released by RSO Records. The song "You're the One That I Want" was a US and UK #1 for stars John Travolta and Olivia Newton-John. It is the second best-selling album ever released and top-selling soundtrack in history with 44.7 million copies sold worldwide.
Besides performers John Travolta and Olivia Newton-John, the album also featured songs by rock n roll revival group Sha Na Na as well as the hit song "Grease", a tune written by Barry Gibb (of The Bee Gees) and sung by Frankie Valli (of The Four Seasons) that was an additional U.S. number one.
The soundtrack was released in April 14, 1978, two months ahead of the film's release. The cover gives credit to, and prominently features, the two stars of the film—John Travolta and Olivia Newton-John—although they only appear on seven of the 24 tracks. The remainder of the album is sung by various cast members and Sha Na Na, a group who performed many of the 1950s numbers in the film. The title track was recorded by Frankie Valli, who had no other connection with the film.
The Guardian | 24 Apr 2019
WorldNews.com | 25 Apr 2019


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A train needs couplers in order to stay together to move the freight down the rails. Our model trains need functioning couplers to successfully complete a similar task. People frequently ask me what couplers are used on the HO scale Wheeling Freight Terminal. Since I just replaced couplers on several models, it seemed like a moment to share in a blog post. Click on any image here to review a larger size.
Tag: freight cars
Southern Pacific A-50-5 automobile box car
Late in 2015 I realized I had several HO scale resin freight car kits that were proper for my 1926 modeling era, but the kits lacked decals that reflected the lettering in use then. This realization downgraded quite a few freight cars on the kit build list. Sometime in the summer of 2016, I stumbled onto a company that produced decals for the Southern Pacific A-50-5 automobile box car that included the as-built lettering. The discovery prompted moving a Funaro & Camerlengo kit into the top of my to-build list.
Continue reading "Southern Pacific A-50-5 automobile box car"
Assisting others: CNJ box car
In mid-February I received an email from a friend who was responding to a question from Alan Mende. Alan needed decal help to finish a box car project and I was added to the email response as a possible resource. Alan was lucky as I had a decal set on hand to send to him. Here's Alan with more details.
I had scratchbuilt a Central Railroad of New Jersey box car from a plan published in the January 1972 issue of Model Railroader. The model reflects the pre-Safety Appliances appearance, which is why there is only one grab iron on the car side. I was planning to use a Clover House dry transfer set, but the HO versions were out of stock. What to do?
Accurail 36-foot box car underframe tutorial
I've seen and heard lots of good comments on the new Accurail HO scale, 36-foot, double sheathed, box car kits. But many people seem puzzled on building the underframe. As an Accurail kit, we have come to expect parts to fall together easily. This kit is no exception; there are just a few more parts to carefully set into place. Follow along on a tutorial to help you assemble the underframe on these fine kits.
Continue reading "Accurail 36-foot box car underframe tutorial"
New Accurail 36-foot Box Car Models
The new Accurail HO scale 36-foot box cars kits are being released. I recently picked up two undecorated car kits with the fishbelly center sills and corrugated metal ends. It's exciting to open the box on a new model for the first time, especially a model that fits into my modeling focus. Let's take a look at this new kit.


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Hi this is Allan Ross from AJ Ross Creative Media
Today I would like to talk about,
When size really does matter… How to look and act bigger to increase business and grow.
We all know the infamous account of David and Goliath, where a young little boy defends himself and wins over the renowned giant. It's the classic 'underdog wins story' and emphasizes the importance of using one's knowledge over one's physical size.
While that may work most of the time–there still is that new business pitch or new venture when you realize–size really does matter.
Here are some tips on how to look and act bigger than you are to land that new business and help your business grow, all without giving up what you went into business for in the first place–your expertise.
First, you need to surround yourself with professionals who think and work like you. I call this the "type wow" personality, and I mean it in a good sense. Owning your own business is fast paced and there is no room for an unmotivated staff. The key is to nurture talent and keep them. Your image is portrayed not only by you, but also by all who work for, and with you.
Image is everything. So, how does a small to mid-sized company compete with the designer, high-end New York City executives? "Stay on top of your game," is the best way to put it. Project an image of confidence and assurance, but be sure to back it with experience, examples and referrals. Know your field, know your client's needs and cater to your client as if he or she is the only one that exists. Larger companies tend to lose sight of client needs, and quickly, once they get the account.
You may have to tweak who you are on occasion. If your office looks more like a storage bin than a workplace, rent space for that big meeting. Bring in the caterer if necessary. Even hire temporary staff. Show that you are interested in entertaining your new client.
Re-think your marketing materials–from your letterhead to your brochure to your web site. Do you present a mom-and-pop image or a new, trend-setting company ready to produce that award-winning widget or provide that phenomenal service? Yes, image. And yes, it's important to have a polished, professional and integrated package to explain who you are and what you do. You need more than just a logo and some ads in the local newspaper. You need a professional marketing company. Make the investment. Hire a company who knows what they are doing, who has experience and a track record. Remember, you get what you pay for. A lot of companies try to save money and hire designers who have no marketing or branding experience. You should also use public relations, which is a tried-and-true approach that should be an integral part of any marketing program. Have the agency write and place announcements of your firms' achievements, new business and new hires, feature articles, and even speaking and seminar engagements.
So, with tape measure in hand, or at least a fresh look at your company and what you do–it's time to move forward. Use your talents, seek advice, outsource, revise your image by hiring the right brand professionals, tell everyone and… succeed.
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| World Cup Brazil
Daily update ⋅ October 1, 2015
FC Goa coach and Brazil great Zico committed to helping 'develop football' across the world
"It's a new challenge for me," said Lucio, who captained Brazil at the 2010 World Cup. "I was selected by Zico because he thinks I can help the group.
Romario Blames Neymar Ban on Brazil FA President's Fear of Arrest Amid FIFA Case
Former Brazil international Romario has put the blame for Neymar's lengthy ... As a result, he will miss Brazil's October World Cup qualifiers against ...
Revealed: what it'll cost the FTSE if England crash out of the Rugby World Cup
If England are knocked out of the Rugby World Cup this weekend, it could ... include England, France, Germany, Italy, Spain, Argentina and Brazil.
Adrift: Brazil's Crisis of Confidence
Brazil fell to Germany 7-1 in the semi-final of last year's FIFA World Cup soccer championship held in Brazil. Since then, things have only gotten worse ...
Ireland fall in world rankings while Wales are just one place behind Brazil
Michael O'Neill's team are also close to reaching Euro 2016 and their first major tournament since the 1982 World Cup. They top Group F after eight ...
Scolari pays €3m in Portugal back taxes
Scolari guided Portugal to the final of the 2004 European Championship and to the semifinals of the 2006 World Cup. He resigned as Brazil manager ...
Petrescu: It was special to score against England
For any player, scoring in a debut FIFA World Cup™ is something to cherish for a life ... The quarter-final finish remains Romania's best World Cup result but ... would have progressed into the last four and set up a meeting with Brazil.
Brazil's Tite wants to coach Italy or Spain
Tite said he received an offer from Inter Milan after guiding Corinthians to the FIFA Club World Cup and Copa Libertadores double in 2012.
Germany back in 2nd place in FIFA rankings, Spain up to 6th
World Cup champion Germany has moved back into second place in the FIFA rankings behind Argentina. The new rankings take into account the ...
Brazil's Marin to be jailed at Trump Tower - report
FIFA World Cup 6 days ago Associated Press ... Former Brazil federation president Jose Maria Marin will pay 40 million Brazilian Reals ($10 million or ...
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Journal Articles
Effect of propofol on ropivacaine metabolism in human liver microsomes
- Author
- Yoshiko Osaka, Shinichi Inomata, Einosuke Tanaka, Takako Nakamura, Katsuya Honda, Masayuki Miyabe, Hidenori Toyooka, Makoto Tanaka
- Journal
- Journal of Anesthesia SCI(E) SCOPUS
- Publisher
- Springer in 2006
- Cited Count
- 0
- Partners
- Springer CiNii MEDLINE®/PubMed® British Library Crossref
- 10.1007/s00540-005-0358-5
- Paid
- Springer CiNii MEDLINE®/PubMed® British Library Crossref
- Library Links
- 꽃동네대학교 서울대학교 남서울대학교 settings
- Category
- Medicine > Anesthesiology and Pain Medicine
- Keywords
- Propofol, Ropivacaine, Metabolism, Human, Microsome, Amides, Chromatography, High Pressure Liquid, Cytochrome P-450 CYP3A, Cytochrome P-450 Enzyme Inhibitors, Cytochrome P-450 Enzyme System, physiology, Drug Interactions, Humans, Microsomes, Liver, pharmacology
- Abstract open button
- References open button
A combination of the general anesthestic propofol and epidural anesthesia with a local anesthetic is widely used. The metabolism of ropivacaine and that of lidocaine are mediated by similar P450 isoforms. Previously, propofol was found to inhibit the metabolism of lidocaine in vitro. Here we investigated whether propofol inhibits the metabolism of ropivacaine using human liver microsomes in vitro. Ropivacaine (6.0 µmol·l−1) as the substrate and propofol (1–100 µmol·l−1) were reacted together using human microsomes. The concentrations of ropivacaine and its major metabolite 2′,6′-pipecoloxylidide (PPX) were measured using high-performance liquid chromatography. The metabolic activity of ropivacaine was reflected in the production of PPX. The inhibitory effects of propofol on ropivacaine metabolism were observed to be dose-dependent. The IC50 of propofol was 34.9 µmol·l−1. Propofol shows a competitive inhibitory effect on the metabolism of ropivacaine (i.e., PPX production mediated by CYP3A4) in human CYP systems in vitro.
References 22
1.The effect of erythromycin, fluvoxamine, and their combination on the pharmacokinetics of ropivacaine.(2000) M J Jokinen et al. ANESTHESIA AND ANALGESIA
2.Propofol decreases the clearance of midazolam by inhibiting CYP3A4: an in vivo and in vitro study.(1999) N Hamaoka et al. Clinical Pharmacology And Therapeutics
3.Propofol: a new intravenous anesthetic.(1989) P S Sebel et al. ANESTHESIOLOGY
4.Human cytochrome P450 mono-oxygenase system is suppressed by propofol.(1995) T L Chen et al. BRITISH JOURNAL OF ANAESTHESIA
6.Inhibition of cytochrome P450 2E1 by propofol in human and porcine liver microsomes.(2002) Corinne Lejus et al. BIOCHEMICAL PHARMACOLOGY
7.Effect of ciprofloxacin on the pharmacokinetics of ropivacaine.(2003) Mika J Jokinen et al. European Journal of Clinical Pharmacology
8.Effect of clarithromycin and itraconazole on the pharmacokinetics of ropivacaine.(2001) M J Jokinen et al. Pharmacology & ToxicologyPharmacology cited 16 times
9.Involvement of human liver cytochrome P4502B6 in the metabolism of propofol.(2001) Y Oda et al. British Journal of Clinical Pharmacology
10.Therapeutic and toxic blood concentrations of more than 800 drugs and other xenobiotics.(2003) M Schulz et al. Die PharmaziePharmacy cited 0 times


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Townsquare Staff
Ohio State Shooting — Suspect Killed by Police After Stabbing Multiple Victims (UPDATED)
Ohio State University was the site of an active-shooter situation Monday morning.
Vikings Absolutely Maul Poor Guy While Running Out of Tunnel
Wrong place, wrong time.
Athletes React to Donald Trump's Stunning Presidential Win
Everyone has an opinion about Donald Trump winning the presidency. Even athletes.
Indians Fan's World Series Champs Tattoo Is a Huge Blunder
Here's a lesson in not putting the cart before the horse.
Hot Rod Car Improbably Wheelies to Stirring Victory
This car gets up there -- and not just in miles per hour.
Pizza Hut Is Hiring the 'Ultimate Sports Fan' to Eat, Travel for $50K a Year
Every sports fan's dream job is about to become a reality for one lucky person.
Rally Car Flawlessly and Stunningly Drifts Into First Place
This move here was pretty drifty.
Chinese Arena Football TD Celebrations Are All-World, All-Pro Ridonculous
It looks like elaborate touchdown celebrations are being outsourced to China.
Clueless Gary Johnson Asks 'What Is Aleppo?,' Could Jeopardize White House Bid
Gary Johnson's presidential campaign may have taken a huge, embarrassing hit.
Jarrod Dyson's Sick HR Robbery Is Gold Glove Glorious
This may be the moment we remember the Kansas City Royals turned their season around.


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Email threat asking for ransom
Complain Detail:-> I got mail from unknown person to my office outlook. he threatening me that he has hacked all my contact and photos. he is demanding 5000USD in bit coin. if i fail to make the payment ,he will release my video and photos to all the contact and colleagues. Please guide me how to get out of it.
- Complained by Kamalakannan
- No of Views 31
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My Facebook friend hacked my Google account and erased my phone
Aditya shukla Sat, 21 Apr 2018 - E-mail Hacking
YouTube video hacking and Hindu Muslim fighting of this video messaging in this my YouTube channel hacked totally fraud
My Gmail is hacking please back to me hacker change all details
Saurabh Chavan Fri, 13 Apr 2018 - E-mail Hacking