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W.H.G. Kingston was a 19th century American writer best known for writing kids adventure novels that were very popular with boys at the time. His books are still widely read today.
LOOK AT YON SMOOTH-FACED BLUE-EYED lad; his fair locks escaping from beneath his broad-brimmed hat stuck to the back of his head; his blue shirt collar, let in with white, turned over his neck-handkerchief, which is tied with long streaming ends; his loose jacket, his wide trousers. You know the sailor lad at a glance. He is a well cared for apprentice under a kind captain. He wins your regard by his artless frank manners, and you think all sailor boys are like him. Then see that fine specimen of a man rolling along, with his huge beard and whiskers, his love locks, his dark flashing eyes, his well bronzed countenance, his bare throat, his dress, similar to that of the lad, but of good quality and cut to a nicety. He looks the hero of the sea, and so he is, and so he feels himself.
What will he not dare and do? He will board a foeman's ship by his captain's side, however few with him or many against him; storm a battery sending forth showers of deadly shot; leap overboard to rescue a shipmate from a watery grave; will lift in his arms a charged shell with the fuzee yet burning, or will carry on his shoulders a wounded comrade from beneath the very guns of the foe. He loves to fight on shore as well as at sea. He will suffer cold, and hunger, and thirst, and face death in a thousand forms without complaint if his officers set him the example. He is the true man-of-war's-man, proud of his calling and despising all others.
Now watch yonder nicely dressed old gentleman, with his three cornered hat, white neckcloth, long blue coat with gold lace cuffs. He is a Greenwich pensioner. He has done his duty to his country and done it well, with all his heart; and now his country, whom he served in his strength and manhood, cares for him as she should in his old age.
From these pleasing pictures people are apt to form their notions of sailors and of a sea life, but there exists another numerous class of whom I have a very different sketch to present.
They are as a class, however, gallant fellows. They also will dare and do all that men can accomplish. Many are kind hearted, generous, brave; but others are too often brutal, fierce, vicious, drunkards, blasphemers, thinking only of present gratifications, and utterly regardless of the future or of the world to come.
If such characteristics be theirs, I have a very solemn question to ask. Why are they so? Who has allowed them to become so? What steps have been taken to improve them? The newspapers often give us one reason why they are brutal. Sent ignorant to sea, ignorant they grow up, no one taking thought for the wellbeing of their souls or bodies; placed often under ignorant brutal masters, whose only idea is how to get the most work out of them, whose only argument is a handspike or rope's end; ill-fed, ill-treated, ill-clothed, ill-lodged (oh what foul, wet, dark holes have thousands of gallant sailors to live in on board ship); ill looked after in sickness; when they return to port, handed over to the tender mercies of crimps and foul harpies of every description, the lives of our merchant seamen are short and hard indeed.
Remember that these are the men who supply us with all the luxuries we enjoy, who have charge of the merchandise which has made England great, glorious, and powerful. Who then, I ask, has an excuse for refusing to support any measure which will benefit them, their souls and their bodies? Can any one deny that our seamen have a claim on the sympathies and aid of every member of the community, whether living in an inland town, in the sequestered village, or on the wild sea side?
Oh could you but behold the merchant seaman on board his ship, the coaster, the trader to neighbouring lands, aye on board some fine looking craft also bound to distant ports; could you see him as he is, day after day toiling on in his tarry, dirty clothes, unshaven, unwashed, with rude companions, obscene in language and habits, in their foul den of a berth; could you hear the expression applied to him by his superiors, his groans of pain, his muttered curses as kicks and blows and cuffs follow after the oaths showered on him; could you see him in port consorting with the vilest of the vile, living in filth and iniquity till his hard-earned gains being spent, his senses steeped in drink, he is put on board another ship, often not knowing where he is going till far out at sea. Could you see and hear, I say, one tenth part of the horrors which take place, unnoticed by man, on the wide ocean, you, my readers, would weep and exclaim, unless your hearts are harder than adamant,
We must, we must do something for that poor fellow's soul and mortal frame.
Before, therefore, I begin the life of Jack Buntline, I must tell you how that something may be done. There exists in London a society called The Missions to Seamen, which I was the humble means of establishing there some five years ago. It had before existed at Bristol. It is warmly supported by numerous admirals, and other naval officers and men of influence. The office is at 11, Buckingham Street, Strand, and the Secretary is the Rev. T.A. Walrond, an excellent clergyman, who has devoted himself with the utmost zeal and energy to the interests of sailors. The object of the Society is to supply clergymen and lay missionaries for seamen: but they do not wait till the sailors come to them, they seek them out on board their ships, not only in harbours and rivers, but even in open roadsteads, such as the Downs, the Solent, and Portland Bay, wherever, indeed, any number of vessels are brought up together. The Society possesses several small vessels, on board which seamen are collected and services are held, as also boats for carrying the missionaries on board the ships. They have a flag, the design of which is an angel carrying the open gospel in her hand, on a blue ground.
The work of the chaplains and missionaries is, as I have said, especially to seek out seamen on board their ships, without waiting for them to come to hear them. They visit them in their berths, however close or foul they may be, read and explain the Bible to them, pray with them, collect them for public worship, and preach to them; offer them Bibles, leave tracts with them, and speak to them as friends whose only desire is for their soul's welfare. Under God's guidance a very large amount of good has, I believe, by these means been done, not only among British, but foreign seamen who visit our ports. Five years ago I was induced to commence the work by the Rev. T.C. Childs, who had succeeded the Rev. Dr Ashley, as sole chaplain of the Bristol Channel Mission, the only one then existing. We have


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To see in Boa Vista
Praia de Santa Mónica, Cape Verde
Santa Monica Beach
Famous for its extraordinary natural beauty, Boa Vista is home to one of Africa's most beautiful beaches: Santa Monica Beach. Located to the south-west and more than 22 km long, it is the ideal destination for excursions and to enjoy Cape Verde in total relax while admiring the turquoise and crystal clear sea.
Deserto de Viana, Cape Verde
Deserto de Viana
In the north-western part of the island stretches a real desert, just one kilometer wide and five kilometers long. The Viana Desert is an extension of the Sahara and is characterized by fine and white sand. You can cross it on foot in complete connection with nature and without any danger. Under the moonlight the show is guaranteed: the naturally lit desert will make the atmosphere unique and unforgettable.
Cabo Santa Maria, Cape Verde
Cabo Santa Maria
In 1968 the ship Santa Maria sank on the beach of Boa Esperança, on the island of Boa Vista. This wreck is the subject of mysteries and legends, some say that the ship sank because the captain fell asleep, others tell of electromagnetic fields that drove the compass crazy and consequently diverted the trajectory of the ship.
Today the beach offers an evocative image that attracts tourists, photographers and painters becoming one of the greatest tourist attractions of the island.
Morro Negro Lighthouse, Boa Vista, Cape Verde
Morro Negro Lighthouse, Boa Vista, Capo Verde
It is the highest lighthouse in Cape Verde and was used to orient sailors approaching east of Cape Verde. Now it is not working, but still allows you to appreciate a breathtaking view.
Ojo Do Mar, Cape Verde
Odjo do Mar
Odjo do Mar is a natural basin that is formed thanks to the autumn rains. It is a small corner of paradise, the pearl of the island, which attracts tourists and Cape Verdeans to spend relaxing day to swim and dive in this small lake.
Sal Rei, Cape Verde
Sal Rei
The chief town of the island of Boavista Sal Rei is a town in which the activity of the country is carried out. Here you will find restaurants, excursions and much more to brighten up your holiday.
praia de estoril
Praia Estoril
Praia Estoril is one of Boavista's equipped beaches where you can relax comfortably while sipping a good mojito. You will find modern beach clubs, restaurants and chiringuitos directly on the beach. Among these, the Tortuga and the Bahia Beach, where you can enjoy excellent dishes and enjoy beautiful moments of relaxation. There are several kite and Wind surf schools that populate the beach where you can attend courses or rent the necessary equipment.
Mercado Municipal, Sal Rei, Cape Verde
Mercado municipal
The largest fruit and vegetable market on the island, a supply point for local people in the center of the Sal Rei square. Here you can buy fresh and tasty products and immerse yourself in the beautiful Cape Verdean atmosphere with the locals. Bananas, pineapples, papaya with a warm and tasty flavor but also vegetables and vegetables.
Rabil, Cape Verde
Rabil is a small town rich in history and tradition. Here you can experience the typical Cape Verdean atmosphere by chatting with the locals and walking through the streets of the village. To remember are the handmade products in terracotta that you can buy in the laboratory-school, present in this small town.
Morro de Areia Nature Reserve, Cape Verde
Morro de Areia
Morro de Areia is not only one of the most beautiful beaches in Boa Vista, it is also a Nature Reserve, home to five species of turtles during the breeding season. Ideal for long and peaceful walks, on this beach you can photograph the rocky spurs on which the waves of the ocean break. It is easily accessible by quad or pick up.
Varandinha, Cape Verde
Varandinha is one of the most popular destinations in Boa Vista, famous for its cliffs overlooking the sea and the famous cave from which you can admire and photograph, at low tide, the landscape outside. It can be reached by pick up or quad and it is recommended to book a trip to discover the wonders of the beach accompanied by an experienced guide.


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Message posted by Mike Sharp on June 29, 2019 at 5:48:44 PST:
I am interested in all the weather monitoring stuff having seen Glen Campbells original videos.
I have software and hardware that I monitor weather with. I am now housebound with respiriatory problems but the old stormchasing toys still work.
Happy for people to contact me to help me further my personal research on weather.
BTW I am based in the UK but am a qualified Spotter
Post a Reply


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Orkney joins Shetland
A previous trip (not sailing sadly) to Shetland gained me a Shetland courtesy flag which hangs from the ceiling of my study and which I took on this trip in case we made it that far (we didn't).
On this voyage to Kirkwall I bought two Orkney courtesy flags, one of which I left on the boat (along with a Caithness flag for when we were in Wick) and the other now hangs in my study.
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Was Determined To Not Take My Own Life, Went For A Journey To Make Mind Peaceful: CI Navas
Kochi: Circle Inspector Navas, who went missing for two days and later returned from Tamil Nadu, said that he went for a journey to make his mind calm. Addressing the media on Sunday, Navas said that he was determined not to take his own life at any cost. It was alleged that he faced mental harassment from senior police officials. He said he met his guru at Ramanathapuram and on his way back, he saw a newspaper report about him, which compelled him to return home as soon as possible so that his kith and kin are not worried.


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- CA BWGPL LHC-FamFil-Lloyd-OS9535
- Item
Edgar, John and Helen Lloyd.
7 results with digital objects Show results with digital objects
Edgar, John and Helen Lloyd.
John, Helen and Edgar Lloyd.
Edgar, John and Helen Lloyd.
Lloyd Family - women and children
(L-R): Edith Lloyd, Mary McDevitt, Laura Lloyd, Sarah Louise (Street) Lloyd, Ruby Fraser Lloyd, Aunt Ann Abott, Myrtle Lloyd Ritchie. Children: John Lloyd, Marilyn Ritchie, Doris Ritchie, Helen Lloyd, Edgar Lloyd and Jim McDevitt.
New Allis Chalmers tractor on the Lloyd farm. John on seat, Steve (hired hand) on top, Walter standing behind, Laura at front and daughter Helen seated by wheel. Tractor was purchased for $500.00 and in approx. 1949/50 sold it for $800.00
Allis Chalmers tractor with John, Helen and Edgar Lloyd in front.
John Lloyd and his second wife Sarah Louise (Street). Parents of Walter Lloyd, grandparents of John, Edgar and Helen.


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Enter your destination and find cats wherever you are!
Four years ago, Phil, one of the owners of Inn On The Lakes in Sebring, Florida would sit at his desk each day and watch a shy tiny little kitten run around outside of the hotel. For one week, he placed food outside in the parking lot for him and slowly moved it closer to the front door. He finally decided to come inside and has been a part of the family ever since.
Part fish, part cat, Dolphinfish is lucky to call Seawall Apartments and the idyllic beachfront at Glenelg in South Australia her home.
Books and cats are our two favourite things. Combine them and you get Agatha, the lovely bookshop tabby who has moved into one of the world's most famous bookshops, Shakespeare and Company in Paris, France.
Meet our new tabby girls Amber and Tabby who are the bosses of Lindeth Fell Country House in The Lakes District, UK. We came across this beautiful property because we spied ginger Amber smooching her little heart out all over guests in some Instagram photos!
Former wild lady Ingwe has turned herself into a five-star cat and can often be found in the grill at 12 Apostles, having a well deserved snack.


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On Friday 12th May 2017, the Absolute team gathered with their road bikes to tackle 40 miles of North Yorkshire countryside in the Carter Jonas LandAid Pedalthon.Read More
Renowned for its pioneering advances in office furniture design, Boss Design continues to innovate with Trinetic - a unique task chair that incorporates a brand new type of movement to create a superior and completely natural user experience.Read More
Our site team have returned to Brindley Way for the second time in twelve months and this time with an enormous space to renovate. The office block has been unused for around a year, which has presented us with a challenge of performing a complete facelift to the building.Read More
Are you looking for the perfect compliment to your reception/waiting area? The Valencia by Morgan Seating offers an elegant, lightweight aesthetic to your office, packed with luxurious detail and comfort.Read More
This year we celebrate ten years of designing and creating great places to work by teaming up with the company that offered us our first professional contract under the Absolute name back in 2006.Read More
You only get one chance to make a first impression. We collaborated with the architect and building manager to try and create a modern, refreshing and memorable first experience to everyone who visits the newly renovated space at 2 City Walk in Leeds.Read More
Absolute were honoured to be offered the opportunity to design and create a new office space for online alcohol retailer, Beerhawk, at their Whetherby HQ.Read More
A momentous achievement for Absolute at the Insider Yorkshire Property Awards 2016 after being crowned Refit and Refurbishment Company of the Year.Read More
The new headquarters of High Street TV, designed by Harrogate company Absolute Contract Interiors, will provide 26,000 sq ft of office space.Read More
We were absolutely thrilled to see so many people in attendance at our 10th Year Anniversary Party at Revolucion De Cuba in Leeds on ThursdayRead More


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The Reynolds Group
"I've partnered with Kathy for many years, and am always impressed by the quality of her work. She is smart, creative and loves all things internet. Whether she's creating a website, designing an email template, writing blog posts for clients or crafting a detailed social media campaign, I can count on Kathy to deliver the highest work quality. Our clients love having her as part of the team because she gets the job done right. Moreover, she's fun to work with. I highly recommend her work and look forward to many more joint ventures together."
Tom Reynolds
Principal, The Reynolds Group


text: Human Rights Observatory Myanmar's Top Court to Rule on Jailed Journalists' Appeal Myanmar's Supreme Court agreed to rule Tuesday on an appeal by two Reuters journalists who have been sentenced to seven years for their news coverage of Myanmar's violent suppression of Rohingya Muslims. Justice Soe Naing adjourned the case without setting a date for the ruling, but Tuesday's decision means the journalists' lawyers and the judge will meet again. The judge could sustain the appeal if he determines legal procedures were violated by lower courts. If such...Read complete article© Voice of America - Tuesday, March 26, 2019


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Safety glass for rolling stocks
The draft National technical regulation on safety glass for rolling stocks (29 page(s), in Vietnamese)
· This draft regulation stipulates the technical requirements and testing methods of safety glass for using as windshield glass, door's glass in rolling stock.
. This draft regulation applies to organizations involved in manufacturing, trading, importing of safety glass; organizations manufacturing, maintaining rolling stock; organizations and individuals involved in inspecting, testing and certificate granting of quality, technical safety and environment protection for safety glass.


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NIST and the National Cancer Institute cosponsored a workshop on August 18–19, 2005, to examine needs for reference materials for early cancer detection. This meeting focused on standards, methods, assays, reagents, and technologies. Needs for plasma and serum proteomics, DNA methylation, and specimen reference collections were discussed, and recommendations from participants were solicited. This report summarizes the discussion and recommendations for proteomics reference materials.
Collection and analysis of comprehensive proteome data will be considerably more challenging than the human genome project. Whereas the human genome encodes ∼22 000 genes(1), the human proteome is far more complex(2)(3). Multiple protein isoforms arise from the same gene by differential splicing, multitudinous posttranslational modifications(4)(5), wider chemical diversity among proteins than nucleic acids, and ex vivo degradation in specimens. All present substantial technical and data analysis challenges unique to proteomics.
Without universal preanalytical processing and data standards for proteomics, comprehensive analysis is problematic(6). For early cancer detection, reports of novel protein biomarkers in plasma and serum have been difficult to reproduce(7). Consequently, proponents of plasma and serum proteomic biomarker discovery have argued for large-scale, international collaboration to optimize efficiency of global efforts(8)(9)(10)(11). To be effective, this endeavor requires standardization, as well as common reference materials.
Genetic heterogeneity of human populations and variability of human plasma and serum proteins represent additional measurement challenges. Superimposed on interindividual biological variation of primary protein sequences are expressional differences that may vary with sex, age, and physiological status (diet, smoking, exercise, hypoxia, medications, and menstrual cycle). Although reference intervals have been defined for many clinically important protein analytes, few new protein biomarkers have been validated(12). A comprehensive catalogue of all human blood proteins, or even normal and abnormal variability, remains a significant unmet challenge for the future.
Current and Future Physical Standards for Proteomics
The metrology community distinguishes primary from secondary standard reference materials(13). NIST (http://www.nist.gov), the United Kingdom's National Institute for Biological Standards and Control (http://www.nibsc.ac.uk/), and the Belgian Institute for Reference Materials and Measurements (http://www.irmm.jrc.be) produce primary reference materials. NIST-certified standard reference materials (SRMs)1 are certified by 2 or more independent measurement methods to reduce measurement bias. Secondary reference materials typically are those produced by commercial sources for widespread distribution. Secondary reference materials are metrologically linked to primary reference materials through a well-characterized value-transfer protocol. SRMs sold by NIST are universally available without restriction. Reference materials can be classified by composition, intended use, and developer/supplier (Table 1⇓ ).
The international Human Proteome Organization (HUPO) Plasma Proteome Project(14)(15) is a major initiative using proteomic reference specimens that has generated extensive interlaboratory data for protein identifications in serum, EDTA-, heparin-, and citrate-anticoagulated human plasma(11).
The Association of Biomolecular Research Facilities (ABRF) (http://www.abrf.org) has developed protein collections for the mass spectrometry community(16). The ABRF, an international society, is dedicated to advancing research biotechnology laboratories. Its Proteomics Standards Research Group supports proteomic standards (materials, data, and procedures) and is currently sponsoring a collaborative project whose goals include development of validated protein standards containing defined proteins, supplying standard mixtures as test sets for member laboratories, and making ABRF standards available to the proteomics community. In collaboration with NIST, ABRF has under way the development of a 3-peptide SRM in which C-terminal arginines mimic tryptic peptides commonly amenable to liquid chromatography–mass spectrometry (LC-MS) analysis. This mixture is designed as a standard for matrix-assisted laser desorption/ionization mass spectrometry, electrospray mass spectrometry, amino acid analysis, HPLC, and capillary electrophoresis. NIST is currently finalizing the certificate of analysis for RM 8327 (Table 1⇑ ).
In addition, individual laboratories have assembled databases for proteomic reference materials (Table 2⇓ ). Data standards are under development in several sites and organizations. For tandem mass spectrometry (MS/MS) data sets, 2 complementary approaches have resulted from international collaboration, mzDATA(17) and mzXML(18). These are undergoing consolidation under the auspices of the HUPO Protein Standards Initiative led by R. Apweiler of the European Bioinformatics Institute (Hinxton, United Kingdom). Such data standards, although critical to technology platform comparisons, have been addressed elsewhere as guidelines for publication(19) and in a tutorial for protein inference from shotgun proteomics(20).
Recommendations for physical standards for plasma and serum proteomics were the focus of the NIST-National Cancer Institute workshop, and the efforts outlined above address specific applications. As the proteomics field approaches clinical applications on a global basis, however, proteomics reference standards with certified purity and metrological rigor are an increasing need that might be addressed by universal consensus plasma and serum proteomics reference materials to benchmark technologies developed for early cancer detection.
Which Reference Materials Are Needed?
Standard mixtures of pure, well-characterized synthetic peptides would be useful for benchmarking MS techniques, quality control for chromatography and capillary electrophoresis, generating MS/MS spectral databases, and enriching analytical samples as internal controls. Standard composition depends on intended use. Peptide reference materials for evaluation and standardization of chromatographic separations should have favorable chromatographic retention characteristics, whereas those for MS should generate mass spectra of established intensity and complexity.
The reference peptide mixtures should have a range of mass and elution times on LC-MS and be free of oxidizable residues that compromise chemical stability. Peptides with posttranslational modifications would also be useful for certain applications. For example, standards for glycopeptide and phosphopeptide identification should contain appropriately defined components for analysis.
A particularly promising new approach uses proteotypic peptides to score proteins for identification and for relative or absolute quantification(21)(22). These peptides, identified from databases or from large empirical studies, including pooled sample studies (http://www.peptideatlas.com), have sequences uniquely matching a single protein and physiochemical properties favoring detection in MS/MS experiments(21). Synthesis and isotopic labeling of such peptides enables large-scale enrichment to generate paired peptide ions and peptide ion fragments from which the relative concentration can be calculated(23).
Cost to end users is also important. Purity and cost must be balanced. In some cases, this will entail high-purity primary standards from NIST and secondary commercially available standards that might be more cost-effective but are still at a purity sufficient for general use.
single proteins
Single proteins, or protein sets, are needed as standards for highly accurate quantitative protein measurements, for which they will facilitate determination of the accuracy of diverse techniques and standardization of assays performed in different laboratories. Proteins might be chosen with clinical relevance in mind. Coordinated high-affinity, highly specific, and well-characterized antibodies against these same proteins would enhance such reference materials, because many current clinical test platforms are based on affinity methods.
Several major protein cancer biomarker tests in blood have been cleared by the FDA for clinical use. These include free and complex prostate-specific antigen for prostate cancer, CA15–3/CA27.29 and HER2/neu for breast cancer, CA125 for ovarian cancer, carcinoembryonic antigen for colorectal cancer, CA19–9 for pancreatic cancer, α-fetoprotein for testicular cancer, and α-fetoprotein-L3 for liver cancer. Many of the CA series are glycosylated variants of the MUC1 gene product and thus problematic for analytical chemical quantification. The WHO and the National Institute for Biological Standards and Control have developed standards for several of these cancer markers.
mixtures of defined proteins
Standards consisting of mixtures of purified and well-characterized proteins allow laboratories to determine the analytical detection limit and specificity of their techniques, compare data across laboratories, and determine the analytical performance of new methods. For example, a standard containing a mixture of 3–5 proteins may be added to study samples to control for tryptic digestion and to detect protein or peptide abundance. These protein standards are also needed to monitor performance and technical variability of instrumentation and as MS mass calibrants for quality control and assurance protocols.
Several workshop participants recommended that varied ratios and concentrations of the protein standard mixtures be used for quantitative MS. As with peptide standards, protein mixtures containing representative phosphoproteins and glycoproteins are needed because of the importance of such posttranslational modifications in cancer-related biochemical pathways that might include biomarkers in future work.
Although commercial sources supply both single proteins and protein mixtures, quality is variable, and frequently, purity and concentration values are not rigorously established. Some investigators have used protein purchased from commercial sources to create standard mixtures. Some workshop participants felt that the purity was not critical in, for example, generation of MS/MS spectra data sets to evaluate computational peptide/protein identification methods, whereas other participants felt that purity was critical for generating MS/MS data sets. Standards for differentiating between samples by LC-MS data will require highly purified proteins with highly reproducible retention times and accurate mass determination.
Anticipated uses of these categories of standards (peptides, proteins, and mixtures) include benchmarking technologically diverse proteomic analysis platforms, quantitative calibration, method development of plasma/serum processing and recovery experiments, and mass calibration for spectrometry measurements of peptides and tryptic peptide digests. However, the standard reference materials listed above are not intended as controls for detecting differences between clinically normal and abnormal specimens.
complex mixtures
Matrix-based reference materials such as serum and plasma address the need for assessment of temporal stability in instrumentation, optimization, and instrument performance and cross-platform comparisons to assess bias. A standardized, pooled serum or plasma preparation would address the majority of community needs for such a complex mixture. Although the volume of serum/plasma from one individual would be limited, plasma pools can supply aliquots linked to proteomic characterization measurements made on the pooled plasma. Homogeneous pooled samples also minimize individual differences in protein concentration. Because of potential interferences, however, pooled serum and plasma are not appropriate for research on autoantibodies. The concentrations of several known proteins in the pooled sera and plasma should be accurately determined and made available along with sample aliquots and documentation of technical details. Prior consensus documents on preparation of pools of human sera and plasma for various uses are available from the CLSI (http://www.nccls.org). Although it is important to define recommendations on standard methods of serum and plasma collection (standard operating procedures) and biological attributes of pool population members, the requirements for each application are so specific that consensus on this level of detail was beyond the scope of the general proteomic standards discussion at this workshop.
For proteomic research related to cancers, it may be advantageous to enrich serum or plasma pools with defined concentrations of ∼15 known cancer biomarkers, such as prostate-specific antigen, CA125, CA19–9, carcinoembryonic antigen, and α-fetoprotein. Consideration of the biological features of the added proteins is important so that unusually unstable proteins or those that might be especially susceptible to proteolysis can be avoided. A balance of catalytic and structural added proteins should be used, and issues such as stable complex formation might also be considered, depending on the assays envisioned for their quantification. Such types of standards are relevant for MS, as well as antibody assays. A series of serum or plasma standards that contain enriched biomarkers at clinically high, low, and intermediate concentrations would be useful, but development of such a series may not be practical. A pool (plasma or serum) that investigators could enrich with various concentrations of the proteins might give the best value for the production effort.
Standardized procedures for plasma and serum specimen collection, processing, aliquoting, and storage are also critically needed. NIST could establish favorable storage conditions and technology. Considering the importance of these processes, it is surprising that there has been no comprehensive survey of related experimental variables, despite the fact that a number of institutions and firms routinely collect large volumes of blood products.
The HUPO Plasma Proteome Project compared results obtained with serum and EDTA-, heparin-, and citrate-anticoagulated plasma specimens from the same donors. EDTA plasma was recommended as the preferred standard specimen, because with its considerable variability, plasma avoids the additional time ex vivo for clotting, and EDTA gave more consistent results with less proteolytic cleavage than heparin- or citrate-anticoagulated plasma(11)(15)(24). For particular analytes, other choices may be indicated. Also, because most archived specimens are sera rather than plasma, interest in serum reference materials will continue(25).
A series of highly purified peptides with appropriate characterization data (purity, homogeneity, reference mass spectra, method of synthesis, web links to relevant technical data, and specific methods for characterization and measurement uncertainties). These would serve as standards for calibration of MS instruments. Various combinations could be used to evaluate hypothesized ion suppression.
A mixture of purified proteins with certification data. For example, FDA-cleared, clinical-grade therapeutic proteins could be supplied with appropriate characterization data (purity, homogeneity, reference tryptic peptide/mass spectra, web links to related technical data, and specific methods used for characterization and measurement uncertainties) to meet needs for analysis of whole proteins or products of tryptic digestion. The diversity of proteins in the mixture and their relative concentrations could be varied for particular experiments. Protein mixtures can be used along with purified proteins of tissue origin to test resolution and dynamic range (e.g., the Institute for Systems Biology 18 nonhuman protein reference material, prepared from commercially available proteins) (Table 2⇑ ).
A human serum or plasma pool of sufficient stability and volume for a 3–5-year supply of 1-mL aliquots. The workshop recommended a survey of potential users to assess their needs. An example currently available is the National Institute for Biological Standards and Control citrated plasma for the International Society for Thrombosis and Hemostasis. Remaining specimens from the HUPO Plasma Proteome Project could meet certain other needs. For collection and pooling from many donors, a major issue is the use of different procedures among institutions, as in clinical or epidemiological settings.
The need for plasma and serum proteomics standards is immediate. After establishment of background measurement methods, most NIST SRM projects take 12–18 months. By collective effort within the global proteomics community and with federal participation, high-grade proteomic standard materials should increase the likelihood of clinical benefits deriving from developments in proteomics.
Certain commercial equipment or materials are identified in this paper to specify adequately the experimental procedures. Such identification does not imply recommendation or endorsement by the NIST, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose. This work was supported in part by NIST-National Cancer Institute Interagency Agreement Y1-CN-45016 (to P.E.B.). G.S.O. is supported by Grant MTTC 687 to the Michigan Proteomics Alliance for Cancer Research, NIH Grant U54DA021519 to the National Center for Integrative Biomedical Informatics, and National Cancer Institute/Scientific Applications International Corp. Contract 23XS110A for the Eastern Consortium for Mouse Models of Human Tumors.
1 CRM, Certified Reference Material; IRMM, Institute for Reference Materials and Measurements; sPRG, Proteomics Standards Research Group.
2 ABRF sPRG proteins: identities of 50 proteins are unavailable until the study is complete (http://www.abrf.org/ResearchGroups/ProteomicsStandardsResearchGroup/Studies/ABRFsPRG2006Study.pdf). Contact: jkowalak{at}mail.nih.gov.
3 CRM470 proteins: transthyretin, albumin, α1-acid glycoprotein, ceruloplasmin, α1-antitrypsin, α1-antichymotrypsin, α2-macroglobulin, haptoglobin, transferrin, complement C3, complement C4, C-reactive protein, IgG, IgA, and IgM(28). Contact: IRMM.
4 SRM 1951a and SRM 1951b content: 50 individual pools each: serum, high cholesterol, low triglycerides, 2 each × 1 mL, frozen; 50 each: serum, low cholesterol, low triglycerides, 2 each × 1 mL, frozen(29)(30). Contact: michael.welch{at}nist.gov.
5 RM 8327 peptides: peptide A: DAEPDILELATGYR (14 amino acids, mw 1561.7); Peptide B: KAQYARSVLLEKDAEPDILELATGYR (26 amino acids; Mr, 2950.3); Peptide C: RQAKVLLYSGR (11 amino acids; Mr, 1289.2)(29). Contact: frederick.schwarz{at}nist.gov.
1 Naturally occurring eukaryotic proteins were purchased from commercial vendors, purified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, excised, and trypsin-digested. Contact: J. R. Strahler, University of Michigan(31).
2 Three hundred recombinant proteins were purchased from a commercial vendor, purified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, excised, and trypsin-digested. Contact: P. Andrews, University of Michigan. Data sets available at http://www.proteomecommons.org/.
3 MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight/time-of-flight. QTOF, quadrupole time of flight mass spectrometer; QSTAR, Applied Biosystems mass spectrometer; ISB, Institute for Systems Biology; NB, sPRG defined in Table 1⇑ , ftn "a".
4 The Opal collection is composed of MS/MS spectra obtained from QTOF mass spectrometers. Only spectra obtained from electrospray ion sources are included in this version (http://www.thegpm.org/quartz). Contact: R. Beavis, Beavis Informatics.
5 The Amethyst collection is composed of MS/MS spectra obtained from QSTAR mass spectrometers. Spectra obtained from both MALDI and electrospray ion sources are included (http://www.thegpm.org/quartz). Contact: R. Beavis, Beavis Informatics.
6 Two protein mixtures, A and B, were formed by mixing together 18 nonhuman proteins purchased from commercial vendors at different molar ratios. The mixtures were trypsin-digested and analyzed by micro-LC-MS/MS(31). Contact: A. Nesvizhskii, Institute for Systems Biology (ISB).
7 Extended from ISB3 mixture. Contains 12 proteins and 23 peptides(32). LC-MS/MS data available for download at http://biatech.org. Contact: K. Kolker, Biatech, 19310 N. Creek Pkwy., Ste. 115, Bothell, WA 98011.
↵1 Nonstandard abbreviations: SRM, standard reference material; RM, reference material; ABRF, Association of Biomolecular Resource Facilities; HUPO, Human Proteome Organization; LC-MS, liquid chromatography–mass spectrometry; MS, mass spectrometry; MS/MS, tandem mass spectrometry.
- © 2006 The American Association for Clinical Chemistry


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6 Valentine's Day Date Ideas To Do in San Antonio
I have a personal belief that how you experienced Valentine's Day in High School impacts the way you see the holiday for the rest of your life. If you were the person who always had a Valentine, got a lot a gifts or generally adored Valentine's Day then you probably have everything in order. If you're like the rest of the population, and you're dating a person who wants you to celebrate, then you could probably use all the help you can get to outside of the box and really wow them.
Be Art Critics at the McNay
With Valentine's Day falling on a Thursday this year you can take advantage of the extra hours the McNay is open and it's also free after 5! Best of all while you're looking at art; the crowds are also thinning out at the restaurants so when you're done you can grab dinner with ease.
Plan a Date Night In
If you're not a fan of the crowds then planning the perfect night in front of the TV should be right up your alley. Order some food in with Favor (use by promo code SALOVELIST for $5 off your delivery) and plan the ultimate netflix and chill at home. But if you want something more romantic then pick up some chocolates and patisserie from Chocollazo, order a sexy charcuterie board from The Board Couple (they're creating a special Cupid's Board for Valentine's Day), and get couple bottles of wines and proceed to set the alarm just early enough for you to call into work the next day. Pro-tip: if you're feeling extra saucy get a room downtown and plan a staycation and if you have the luxury to be disconnected for the weekend then put those phones away and forget about the world for a while.
Surprise Your Honey at Work
Want to make your babe the envy of all the office? Pre-order a bouquet from The Vintage Bouquet Bar to secure your flowers (when I say pre-order I mean it, they're gonna sell out and you'll have to get her a bouquet of baby's breath). They were inspired by the Regency/ Victorian Era so think the Brontë Sisters and Jane Austen and are so romantic (they're the ones on the cover image). Or maybe your babe is into donuts? Order some donuts from The Art of the Donut to satisfy their inner Homer Simpson. If you really wanna get the oooh and ahhhs from her coworkers get her set up with an "I Love You," balloon arrangement from the Balloon Boutique. Get the romance started early, right?
Go Back in Time at the Witte
Bust out the vintage attire and prepare yourself to experience a night of cabaret, jazz, delicious food and an open bar. Make it a one stop shop at the Witte's inaugural Vintage Valentine's speakeasy event. Though I wonder if they have a password? Since it's an open bar, please plan accordingly and order a Lyft, plz.
Meet Your Sweetheart at Dorćol
With two different seating times you'll have the option to eat dessert before dinner or after dinner at Dorcol's Dessert's First event. Between you and me I'd say start with dessert and then go home for the… um… meal.
Have an Indecisive Partner…
and a lot of time on your hands? Then plan a progressive dinner and get a little bit of everything and satisfy your indecisiveness. Plan it around your faves from each restaurant and get the party started, just make sure everything is within walking distance for convenience. I'll get you a cheat sheet so you can seem like a champ with the "plans."
Drinks at Hot Joy
Dinner at Battalion
Gelato at South a la Mode
Drinks at The General Public
Dinner at Yellow Fish Sushi
Dessert at Steel City Pops
Wine at High Street Wine
Oyster's at Southerleigh's Outdoor Oyster Bar
Charcuterie at Cured
Ice Cream at Lick


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My Delusional Cousin
My cousin thinks she is a mermaid. She is 22 years old, and will tell you right to your face that she has been a mermaid since she was 11 years old, when she used a youtube spell to turn herself into one. She constantly wears a pearl necklace because that's where all her mermaid power is stored, and if it breaks, she will fall ill or possibly die.
Apparently, everyone can't see her tail because we don't 100% believe her. She says that her mermaid life is difficult because she doesn't live by any large bodies of water. She has this ritual where she fills a bathtub with salt water (table salt + regular nasty tub water), and bathes in it for HOURS at a time, every full moon. She does it out of fear that, if she doesn't, the spell will stop working.
In other words... For 11 years, she has been living in this fantasy world that this youtube spell has turned her into a mermaid. She has told boyfriends, family, and so many strangers, that she is a mermaid. Strangers either laugh or think she is crazy, and she gets super upset about it. She doesn't understand why people aren't more accepting of "her kind".
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As long as she doesn't reproduce. We've got way too many idiots on this planet as it is, she's just one example
Lol. Trans mermaid.
AHHHH!!! TRANSPHOBE!!!! *cries* *wets pants*
I s*** you not, my neighbour thinks he's a pirate.
He's near 50years old and it's b***** embarrassing
I am hoping that we will get laws enacted to protect holders of delusions against discrimination.
If the social justice tards get their way, you might get your wish. Get ready to learn how to properly address all 978 genders the way they demand.


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The National Drug Law Enforcement Agency says it will adopt task force approach in combating drug abuse and trafficking in the country.
Jonah Achema, Head, Public Affairs of the agency, told the News Agency of Nigeria on Friday in Abuja that the squad would raid traffickers' black spots and hideouts across in the country.
Achema said tblack spots for drugs were spread in many areas in the country and required stringent measures to tackle.
He said: "In the realization of this, the agency has put up a task force that will check nooks and crannies of the country.
"Black spots and hideouts in the Federal Capital Territory such as Zone 4, Apo roundabout, Bannex, Jabi and other areas in the outskirts of the FCT would be raided first.
"I am very convinced that all manner of drugs will be recovered during this operation and good testimonies from the residents of the territory will follow."
According to Achema, the task force is part of the agency's efforts in tackling drug vendors and traffickers in the territory and environs.
He said the agency would achieve the goal by ensuring that the demand and supply of cocaine and cannabis sativa were reduced to the barest.
"The agency has chosen to tackle the menace of drug abuse and ridding the territory with this new mode," he added.
Achema appealed to residents of the FCT to join hands with NDLEA in ensuring a responsible society devoid of social vices.
He said: "The public needs to understand that they should also support the NDLEA in tackling this drug menace."


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Groups Join Forces to Donate Heating Unit to Charlottesville Woman
CHARLOTTESVILLE, Va. (WVIR) - A woman in Charlottesville has been living without heat in her home since November.
Now, she'll be able to stay warm thanks to the generosity of the community.
Joyce Calloway has been getting by with heaters and blankets, but now she has a new air conditioner and furnace unit to make up for all those chilly nights.
The donation was made possible by the first-ever Beck Cohen Cares Day.
Calloway says the heating system she previously had started acting up halfway through winter and it didn't make the house as warm as she would've liked, and this new one is much better.
"I'm really happy about it," Calloway said.
She applied for assistance from Albemarle Housing Improvement (AHIP) back in November and says she was surprised when she received help so quickly.
AHIP partnered with Beck Cohen to get the furnace donated by Lennox Industries.
"We have about 400 families – households - on our waiting list in Charlottesville and Albemarle, so we work in the city and the county, so it can be difficult to choose people," Jennifer Jacobs, the executive director of AHIP, said.
The project cost almost $11,000, but Calloway won't have to pay a penny.
Ten more families living in Charlottesville and Albemarle County will also receive assistance.


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Download: Atlas Genius - "Trojans" (MOVES Remix)
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(March 6-15, 2020) On this education abroad program during spring break, students develop cultural ... with EARTH University's rural development programs. Program Information Previous Experiences ... Australia: Human Impacts on the Natural Environment (May 6-29, 2020) This May program has students exploring ...
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That we are absolved from all allegiance to the existing political parties of this country, and that as free and independent producers we shall endeavor to acquire the full power to make our own laws, manage our own production, and govern ourselves, acknowledging no rights without duties, no duties without rights.
But as Shiite Extremists eager for the overthrow by any means of all police, ayatollahs, bankers, executioners, priests, etc. Schools should not spend money on subjects of fine arts.
Dorr's arrest was ordered by the regular governor, and he went into hiding outside the state, trying to raise military support. After the collapse, hundreds of fighters streamed out of the cities and headed for the mountains.
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We have no quarrel with the concept of culture--including technology; for us the problem begins with civilization. Were the Kallikaks onto something good? The people of El Mozote would have no problems — provided they stayed where they were.
Five hundred soldiers arrived and Baltimore quieted down. The police fired again and again, and when it was over, and the dead were counted, they were, as usual, workingmen and boys, eighteen of them, their skulls smashed by clubs, their vital organs pierced by gunfire. When recruiting for the army began in Julya mob in New York wrecked the main recruiting station.
School colors equal school pride A third argument in favor of school uniforms is that they increase a sense of unity, school pride, community spirit.
The villagers of El Mozote had the misfortune to find themselves in the path of the Salvadoran Army's anti-Communist crusade. Jackson was the first President to master the liberal rhetoric-to speak for the common man.
Jossey-Bass,p. Schools having the same sex students are much better. They describe the Boston rich: Four miles south of El Mozote, outside the hamlet of La Guacamaya, the guerrillas of the People's Revolutionary Army also awaited the soldiers.
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The whole institution of Law saw nothing out of the way in these conditions, and very significantly so, because, to repeat over and over again, Law did not represent the ethics or ideals of advanced humanity; it exactly reflected, as a pool reflects the sky, the demands and self-interest of the growing propertied classes When the day came on which the girls were to turn out, those in the upper rooms started first, and so many of them left that our mill was at once shut down.
Private policies that should be held by the companies of social media. Fans should be censored or not? Punishment for young children on competing at athletics. And it wasn't because the guerrillas were so good; it was because the Army was so bad.
America should refrain from providing foreign aid.In a remote corner of El Salvador, investigators uncovered the remains of a horrible crime — a crime that Washington had long denied.
The villagers of El Mozote had the misfortune to find themselves in the path of the Salvadoran Army's anti-Communist crusade. This Article Includes.
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By Lt Daniel Furseth. Today, I stopped caring about my fellow man. I stopped caring about my community, my neighbors, and those I serve. I stopped caring today because a once noble profession has become despised, hated, distrusted, and mostly unwanted.
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Juneau Rendezvous
March 5, 2019
Due to suboptimal conditions at the venue, this Rendezvous was abbreviated following announcement of release of UAA Outdoor Recreation Industry Economic Impact study
Opportunities in Juneau
Attracting independent travelers
Creation of common group clubs, recreation interest ( e.g., ski, kayak, running, etc.)
Post-rec activity - socializing at local restaurants, bars, picnics, etc.
Kayak guiding
Charter fishing
Trail running
Skiing Eagle Crest
Wildlife tours – whales, birds, bears, Mtn. goats
Wilderness camping – hut trips, wild beaches
Eating and harvesting wild foods
Focus on people's wilderness abilities
Competitive recreation leagues (softball, baseball, rugby, beach volleyball
Building hut to hut opportunities
Ice Caves
Nordic skiing, winter tourism
Increasing car and auto accessibility and capacity – Auke Bay
Historical tours of Treadwell Mine Area
Challenges in Juneau
Balancing local and tourist interests
Perception of exceeding limits of acceptable change
(Note: infrastructure ……..????….disperse cruise ship passengers)
Limited sporting goods, vendors to adequately support expansion of rec. activities
(could be difficulties in operating a viable business locally)
USFS capacity to manage visitor access
More access to public land
Funding for recreation


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