text:
These days, it's extraordinary to see somebody's face, not to mention look. Everybody is on their telephones. They're messaging, Facebooking, Snapchatt Clone Script, Tweeting, taking pictures and recordings. It's quick satisfaction. Now and again it's irritating. It's the better approach to showcase your business. You should grasp it and use it for good!
More individuals, Millennials specifically however not exclusively, convey by instant message. I'm in my 40's (sshhh) and I can disclose to you that the most ideal approach to connect with me is through content. Retailers know this and are gaining by it. Consistently, and I'm not overstating, I get a content from one of my preferred organizations with deal cautions and such. I likewise get notices about neighborhood crises or street terminations in my general vicinity. What's more, as somebody that likes to keep up on breaking news, I likewise get news alarms. This technique for notice is helpful and to the point. When you're occupied, as a large portion of us seem to be, that is a tremendous in addition to.
You may as of now use messages to get the word out about happenings in your business, which is incredible. Be that as it may, in the event that you need to move to the following level and increment your chances of being seen, consider content promoting. As referenced before, writings are quick and are opened quicker than email. Individuals get a plenitude of messages each day. Hence, advertising messages get overlooked substantially more much of the time. I'm not implying that you should stop that road, rather, add on to it.
An incredible aspect regarding content showcasing is that the client (or potential client) selects in. This gives you authorization to send SMS instant messages. The extraordinary thing about pick in prospects, is that they are indicating enthusiasm for what you're offering by joining. This makes the "lead" a lot hotter and bound to get results.
At that point there's the straightforward perspective we cherish to such an extent. An instant message is commonly a sentence or two. The probability of a content being perused is a lot more noteworthy. Incorporate whatever advancement you're running and any "rebate codes" if relevant. Incorporate a connection to guide them where to go to see further subtleties inside that content. That is all the data you have to give and your client needs to see.
Content showcasing is likewise reasonable! There are numerous stages that offer gathering content projects. Much like sending messages, you'll have a rundown that is utilized to send one content to the majority of your selected in contacts. You can even timetable when a content is going out. I adore planned messaging. Commonly I consider something at an insane hour of the day (or night). It's generally not a perfect time to send messages however. I develop a content and calendar it for an increasingly sensible hour, since I don't generally believe that I'll recall. It's splendid.
Making things one more stride, you can use writings to get input from your clients. You may have gotten reviews from your mobile phone supplier, for instance, getting some information about their administration. It's an intuitive device that enables them to pose inquiries and for the client to reply as they're capable. Individuals being occupied, won't generally endure one a 10-minute online overview. Be that as it may, with content reviews, we can respond to an inquiry and not answer the following one till we return to our telephones.
Since a great many people are proprietors of cell phones, you can contact more individuals than any time in recent memory. There are numerous assets online to discover programs for gathering content battles. You'll additionally have the option to discover a few thoughts on what kinds of advancements get the best outcomes. What's more, in the event that you have a Millennial working for you, why not assign that undertaking to them!


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In this lesson, we're going to take a look at three important SketchUp tools—the Dimensioning tool, the Protractor tool, and the Sectioning tool.
The Dimensioning tool lets you display dimensions in your model, as you can see in Figure 10.1.
Figure 10.1. Displaying dimensions.
The Dimensioning tool is a great one when you're creating plans of any sort, such as architectural plans, and need to indicate distances.
The Protractor tool lets you do for angles what the Tape Measure tool (see Lesson 9, "X-Raying Objects, Creating Guides and Offsets") lets you do for straight ...
No credit card required

subset: commoncrawl


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Director Quentin Tarantino returned to Comic-Con today to present his highly-anticipated Western The Hateful Eight with a Hall H panel. The director offered seven minutes of new footage, which has not been officially released yet, but he also debuted a brand new poster. He was joined by seven of The Hateful Eight, Kurt Russell, Jennifer Jason Leigh, Walton Goggins, Demián Bichir, Tim Roth, Michael Madsen and Bruce Dern, although Samuel L. Jackson wasn't present.
Among the highlights of the panel was Quentin Tarantino confirming that legendary composer Ennio Morricone will compose the score for The Hateful Eight, which will be his first Western movie in 40 years. During the Q&A session, Bruce Dern revealed that the director has the greatest attention to detail of any living filmmaker.
"Quentin has greatest attention to detail as any director who ever lived. If he had a rival it would only be Luchino Visconti."
The director also teased plans for making another Western.
Related: Kurt Russell Destroyed a Priceless Antique While Shooting The Hateful Eight
"You have to make at least three westerns to call yourself a western director...I will eventually make 3 westerns."
He also addressed the infamous script leak by Gawker, which almost caused him to abandon the entire production.
"What pissed me off (is) it wasn't finished. Still three drafts to go."
In The Hateful Eight, set six or eight or twelve years after the Civil War, a stagecoach hurtles through the wintry Wyoming landscape. The passengers, bounty hunter John Ruth (NE41kvci4H9k69) and his fugitive Daisy Domergue (Jennifer Jason Leigh), race towards the town of Red Rock where Ruth, known in these parts as "The Hangman," will bring Domergue to justice. Along the road, they encounter two strangers: Major Marquis Warren (Sammuel L. Jackson), a black former union soldier turned infamous bounty hunter, and Chris Mannix (Walton Goggins), a southern renegade who claims to be the town's new Sheriff. Losing their lead on the blizzard, Ruth, Domergue, Warren and Mannix seek refuge at Minnie's Haberdashery, a stagecoach stopover on a mountain pass. When they arrive at Minnie's, they are greeted not by the proprietor but by four unfamiliar faces. Bob (Demián Bichir), who's taking care of Minnie's while she's visiting her mother, is holed up with Oswaldo Mobray (Tim Roth), the hangman of Red Rock, cow-puncher Joe Gage (MMichael Madsen), and Confederate General Sanford Smithers (Bruce Dern). As the storm overtakes the mountainside stopover, our eight travelers come to learn they may not make it to Red Rock after all... Check out the latest poster, and stay tuned for more on The Hateful Eight, arriving in theaters Christmas Day.
Cast of "Hateful Eight" at Comic-Con #SDCCpic.twitter.com/lIoifv1fT0— Stuart Oldham (@s_oldham) July 11, 2015


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This review of Internet-based interventions to support informal carers of people with dementia found low quality evidence that these interventions may improve carer well-being. The authors' cautious conclusion incorporates the uncertainty resulting from the poor quality of the included studies.
To assess the effectiveness, feasibility, and quality of Internet-based interventions for informal carers of people with dementia.
Five databases (including PubMed and PsycINFO) were searched up to January 2013 for publications written in English and Dutch. Search terms were reported. References of included studies and relevant review articles were also searched. Study authors were contacted to assess whether the studies met the inclusion criteria.
Studies that assessed the effectiveness of Internet-based interventions aimed at informal carers (such as spouses, children or children-in-law) of people with dementia or mild cognitive impairment were eligible for inclusion. Studies were excluded if they were aimed solely at the patient rather than the caregiver.
The following interventions were used in the included studies: a web site with information and support for carers on various aspects of caring; a web site with additional carer strategies; a web site combined with telephone support, and a web site with a combination of individual work and exchange with other carers online. The intensity/extent of active support and the duration of the interventions varied widely. All studies focused on more than one carer function-related outcome; these included self-efficacy, stress/burden, depressive symptoms, coping, social contact/support, knowledge, use of health services, and general (mental) health.
Two reviewers were involved in the study selection. Any disagreements were discussed with a third reviewer.
The quality of the studies was assessed using the Cochrane Back Review Group list which included seven criteria based on descriptive, statistical, and internal validity. The scores ranged from 0 to 9. Studies were considered high quality if at least four items scored positively.
The level of evidence was graded from 1 (highest) to 5 (lowest) based on the quality of evidence developed by the Oxford Centre of Evidence-based Medicine (OCEBM) guidelines.
Two reviewers independently performed quality assessment. Any disagreements were resolved by a third reviewer.
Participant characteristics and outcome data were extracted using a standardised data extraction form.
The authors did not state how many reviewers were involved in data extraction.
A narrative review was conducted.
Twelve studies were included in the review (range 11 to 700 participants). The methodology quality of the included studies varied. The observed total internal validity score ranged from 0 to 5 out of 9. Only two out of 12 studies were considered to be of good quality. The follow-up ranged from 30 days to 12 months.
Six studies reported significant improvements in carers' well-being with Internet-based intervention for depression, sense of competence, decision-making confidence, self-efficacy, and burden. One study found increased intention to seek support, increased carer gain, and decreased carer stress and strain. One study reported increased carer self-control. In addition to online interventions, guidance from a coach (two studies) and interaction with other carers (two studies) could be beneficial.
There were no significant differences between or within treatment groups for carer quality of life, use of stress management techniques, social isolation, and health status.
Several studies reported carers' experience as additional outcomes; these included increased knowledge of the disease and caring (three studies), increased coping with caring (two studies), confidence in carer skills (three studies) and reduced feelings of isolation (one study).
Internet interventions for informal carers of people with dementia may improve carer well-being. However, the available supporting evidence lacked methodological quality.
The review question and inclusion criteria were clear. Relevant sources were searched but unpublished studies were not sought, so relevant evidence may have been missed. No formal assessment was made of publication bias but the authors reported that there might be a possibility of publication bias. Methods were used to minimise reviewer errors and bias in the study selection and quality assessment; it was unclear whether similar steps were taken for data extraction.
Study quality was assessed using specified criteria; the results were reported. A narrative synthesis was appropriate because of the diversity of the studies. However, it was sometimes unclear whether statistically significant differences between groups or pre-post measurements were reported. The authors acknowledged the limitations of this review in the complex and inconsistent designs and interventions, and the low quality of many studies.
The authors' cautious conclusion incorporates the uncertainty resulting from the low quality of the included studies.
Practice: The authors stated that multi-component Internet-based interventions combining tailored information with interaction among carers showed the most promise for improvement.
Research: The authors stated that more randomised controlled studies that assessed interventions performed according to protocol were needed to give stronger statements about the effects of supportive Internet-based interventions and their most promising elements.
Alzheimer Nederland, The Netherlands; Alzheimer Research Fund Limburg, The Netherlands; the Faculty of Health, Medicine, and Life Sciences of Maastricht University, The Netherlands.


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Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins
Communicated by Randy Schekman, University of California, Berkeley, El Cerrito, CA (received for review September 19, 1996)
Rer1p, a Golgi membrane protein, is required for the correct localization of an endoplasmic reticulum (ER) membrane protein, Sec12p, by a retrieval mechanism from the cis-Golgi to the ER. To test whether or not the role of Rer1p is common to multiple ER membrane proteins, we examined the localization of two other ER membrane proteins, Sec71p and Sec63p, in the wild-type and rer1 mutant yeast cells, using their fusions with an α-mating factor precursor (Mfα1p). Although Sec71p and Sec63p have completely different topology from Sec12p, their Mfα1p fusion proteins were also mislocalized to the trans-Golgi in the rer1 mutant. Overexpression of these fusions caused their mislocalization to the trans-Golgi even in the wild-type cells, and this mislocalization was partially suppressed by the co-overexpression of Rer1p. Either Sec71p or an artificial chimeric protein whose ER localization depends on Rer1p gave a competitive effect on the localization of the Mfα1-Sec71p fusion, which was abolished in rer1. Thus, Rer1p appears to be one of the common limiting components in the retrieval machinery for ER membrane proteins. The results also suggest that Sec71p and Sec63p depend on ER-Golgi recycling, at least partly, for ER localization. We also examined the effect of a mutation in α-COP, a subunit of yeast coatomer, on the localization of these ER membrane proteins. The Mfα1p fusions of Sec12p, Sec71p, and Sec63p were all more or less mislocalized in ret1–1. These observations imply that the roles of Rer1p and coatomer are much more general than thought before.
Organelles in the secretory pathway are connected by dynamic membrane traffic. Each protein that functions in a particular organelle must have a specific signal for its localization. To date, several signals have been identified for the localization of proteins in the endoplasmic reticulum (ER). Many ER lumenal proteins have a KDEL or similar signal at their C terminus (1, 2). This signal is recognized by its receptor in the Golgi apparatus, which results in the recycling of KDEL proteins back to the ER (3–5). Some ER membrane proteins carry a dilysine or diarginine motif at their cytoplasmic tail, which is also believed to act as a retrieval signal (6, 7). It has been shown that coatomer (COP I) binds to the dilysine motif (8), and mutations in COP I subunits cause mislocalization of dilysine-tagged marker proteins (9, 10). However, there are many ER membrane proteins that contain none of these signals. The mechanisms to localize these proteins to the ER remain largely unknown.
Yeast Sec12p is a type II transmembrane glycoprotein required for the formation of transport vesicles from the ER and does not harbor any signals described above (11, 12). To clarify the molecular mechanisms of ER localization of Sec12p, we isolated two recessive mutants, rer1 and rer2, which have a defect in the proper localization of Sec12-Mfα1 fusion protein (S12Mp) (13). The rer1 mutant has been demonstrated to mislocalize not only S12Mp but the authentic Sec12p as well. The RER1 gene encodes a protein of 188 amino acid residues containing four membrane-spanning domains (14, 15). Rer1p is localized in the early region of Golgi apparatus and, thus, should be involved in dynamic retrieval of Sec12p from the Golgi apparatus. By systematic analyses using various chimeras with a vacuolar membrane protein, Dap2p, we have demonstrated that the cytoplasmic and transmembrane domains of Sec12p contain an RER1-independent signal for static retention and an RER1-dependent retrieval signal, respectively (16).
In this study, we focused on two different types of ER membrane proteins and applied our method using Mfα1p fusions to monitor their behaviors in vivo. One is Sec71p, a type III membrane glycoprotein (17), and the other is Sec63p, which spans the ER membrane three times. Both of these molecules have quite different topology from Sec12p; their N termini are facing the ER lumen and the C termini are cytoplasmic (18–20). They form a multimeric complex required for the posttranslational translocation of newly synthesized secretory proteins (21–28). Here, we show that Rer1p is required for the correct localization of Sec71p and Sec63p as well. Furthermore, there is an apparent competition for Rer1p between these proteins, suggesting that Rer1p is a limiting component of the cellular machinery involved in retrieval. The role of Rer1p will be discussed from the viewpoint of localization mechanisms for various types of ER membrane proteins.
Yeast Strains and Culture Condition.
Saccharomyces cerevisiae strains used in this study are listed in Table 1. Yeast cells were grown in YPD [1% (wt/vol) Bacto yeast extract (Difco), 2% (wt/vol) polypeptone (Nihon Seiyaku, Tokyo) and 2% (wt/vol) glucose] or in MVD [0.67% yeast nitrogen base without amino acids (Difco) and 2% glucose] medium supplemented appropriately. MCD medium is MVD containing 0.5% casamino acids (Difco).
Plasmid Construction.
SEC71 and SEC63-myc genes (19, 20) were kindly provided by D. Feldheim and R. Schekman of the University of California at Berkeley. SEC63-myc contains a DNA fragment encoding the human c-myc epitope (N-LEEQKLISEEDLLRKR-C) just before the stop codon. To insert the Mfα1p peptide at the N termini of Sec71p and Sec63-mycp, a BstBI site was introduced just after the start codon by site-directed mutagenesis with a synthetic oligonucleotide 5′-GAGTTTGCCAATATGTTCGAATTTAATGAAACA-3′ or by PCR mutagenesis with synthetic oligonucleotides: M1320, 5′-GTAAAACGACGGCCAGTGAG-3′; SEC631, 5′-CATACTCGTAATTTGTTTCGAAAGGCATTGTGCTGTA-3′; SEC632, 5′-TACAGCACAATGCCTTTCGAAACAAATTACGAGTATG-3′; and M13R, 5′-GGAAACAGCTATGACCATG-3′. The resulting genes were named SEC71-B and SEC63-myc-B, respectively. In SEC71-B, a NheI site was also introduced just before the stop codon by PCR mutagenesis with M1320, M13R, SEC71C3 (5′-AATAATGATGGAAGATTAGCTAGCTAGTGCCTACTGTGTGCA-3′), and SEC71C4 (5′-TGCACACAGTAGGCACTAGCTAGCTAATCTTCCATCATTATT-3′). A DNA fragment encoding three copies of the HA epitope (YPYDVPDYA) (14) was inserted into this NheI site of SEC71-B. This tagged gene was named SEC71B-3HA. Various-length MFα1 DNA fragments were synthesized by PCR with the following primers: MFα1L, 5′-CCCATCGATAGATTTCCTTCAATTTTT-3′ and 5′-GGGTTCGAAGGGTTTTAACTGCAACCA-3′; MFα1M, 5′-CCATCGATGAAACGGCACAAATT-3′ and 5′-GGATCGATGGGTTTTAACTGCAACCA-3′; and MFα1S, 5′-CCTTCGAAGTACATTGGTTGGCCGGGTTT-3′ and 5′-CCATCGATAAAAGAGAGGCTGAAGCTTGG-3′. The ClaI-digested MFα1M fragment was inserted into the BstBI-digested SEC71-B-3HA and the resulting fusion gene was named MS71H. The ClaI-BstBI-digested MFα1L and MFα1S and the ClaI-digested MFα1M fragment were inserted into the BstBI-digested SEC63-myc-B to produce MS63L, MS63S, and MS63M, respectively. These fusion genes were expressed on a single-copy plasmid pRS316 (29) or a multicopy plasmid pYO326 (30). For the construction of GAP-MS71H, the 0.8-kb SpeI-EcoRI promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (TDH2) was inserted in place of the SpeI-EcoRI fragment of the SEC71 promoter in MS71H. For the construction of 3HA-SEC71, a DNA linker (CGCTAG) was inserted in the BstBI site of SEC71-B to create a NheI site. The DNA fragment encoding the 3HA epitope was integrated at this NheI site. The resulting gene (3HA-SEC71) complemented the temperature-sensitive growth of Δsec71 completely and was thus used as a functional SEC71.
DNA manipulations, including restriction enzyme digestions, ligations, plasmids isolation, and E. coli transformation, were carried out by standard methods. Yeast transformation was performed by a lithium thiocyanate method (31). DNA fragments were purified from agarose gel pieces using the DNA PREP kit (Asahi, Tokyo). DNA nucleotide sequences were determined by the dideoxy method (32) using a DNA sequencer (Model 373A; Applied Biosystems, Japan).
Immunofluorescence Microscopy.
Indirect immunofluorescence was performed as described previously (14, 33). The staining of the HA-tagged MS71Hp and myc-tagged MS63Lp were observed by using the 12CA5 and 9E10 monoclonal antibodies, respectively. Decoration of these antibodies was performed by the addition of 5 μg/ml biotinylated goat anti-mouse antibody followed by 5 μg/ml streptavidin-fluorescein.
Halo Assay.
Halo assay was performed on MCD plates with a tester MATa sst2 strain as described previously (13). Eight independent spots were examined to quantify the amount of α-factor secreted in the all experiments.
Pulse–Chase Experiments.
Metabolic labeling of yeast cells, preparation of cell extracts and immunoprecipitation were performed as described previously (33). A monoclonal anti-HA antibody (12CA5) was used to immunoprecipitate MS71Hp from cell extracts. This immunoprecipitate was dissolved in 1% SDS, divided into three aliquots, diluted with 10× volume of 2% Triton X-100, and subjected to the second immunoprecipitation with the anti-HA antibody, anti-α1→6 mannose antiserum or anti-α1→3 mannose antiserum. Endoglycosidase H treatment and analysis by SDS/PAGE and fluorography were performed as described (34). Radioimage was observed and quantified with a Fuji Film image analyzer BAS-1000.
Construction of Fusions Between Mfα1p and ER Membrane Proteins.
In our previous study, we devised a method using a fusion protein between Sec12p and a yeast α-mating factor precursor (Mfα1p) to monitor its localization in vivo (13). When this fusion protein is transported to the trans region of the Golgi apparatus, three processing proteases act consecutively on the Mfα1p moiety and produce the mature α-factor. The secretion of α-factor can be easily detected by the halo assay. This method has been successfully applied to the analysis of the rer1 mutant (13, 14) and identification of the ER localization signals of Sec12p (16). In the present study, we employed the same strategy to test the generality of RER1 function for ER protein localization. The ER membrane proteins chosen as markers are Sec71p and Sec63p. The constructs we made as fusions with Mfα1p are shown in Fig. 1.
SEC71 encodes a 31.5-kDa ER membrane glycoprotein of type III topology, which is completely opposite to Sec12p. The SEC71-disrupted mutant shows temperature-sensitive growth. MS71Hp was designed to contain an Mfα1p derivative without the signal sequence at the N terminus of Sec71–3HAp, because the authentic SEC71 product does not carry a cleavable signal sequence at the N terminus. The MS71H gene complemented the temperature-sensitive growth of Δsec71 (RSY926) at 37°C on either a single-copy or multicopy plasmid as well as the bona fide SEC71 (data not shown). Immunofluorescence microscopy showed that MS71Hp was correctly localized to the ER at steady state (Fig. 2A–C). These results indicate that MS71Hp remains fully functional as Sec71p in the ER.
SEC63 encodes a 73-kDa ER membrane protein, which spans the ER membrane three times (see Fig. 1). First, we inserted an Mfα1p peptide without the signal sequence at the N terminus of Sec63p, but this fusion failed to complement the temperature sensitivity of the sec63–1 mutant (RSY151). We constructed two more fusions, MS63Sp and MS63Lp, which contained four repeats of the mature α-factor peptide only or the whole polypeptide of prepro-α-factor, respectively. Unfortunately, these fusions did not complement sec63–1 either. Nevertheless, because we could observe clear ER localization of MS63Lp (Fig. 2D–F), we decided to analyze its behavior in terms of Rer1p dependency of localization.
RER1 Is Important for ER Localization of Multiple Membrane Proteins.
We examined the localization of these fusion proteins by measuring α-factor secretion by the halo assay. As described previously (16), the size of the halo is a quantitative measure of the amount of the fusion protein that has arrived at the trans-Golgi. The three fusions, S12Mp, MS71Hp, and MS63Lp, were introduced into RER1 (SNY9) and Δrer1 (SKY7) cells on a single-copy (CEN) or multicopy (2μ) plasmid and subjected to the halo assay (Fig. 3). As shown in our previous studies (13, 14), S12Mp produced no halo in RER1 but formed a halo in Δrer1 in a dose-dependent manner. In contrast, MS71Hp and MS63Lp made a large halo even in the wild-type cells (RER1 RET1) when they were on the multicopy plasmid. This suggests that the capacities of their ER localization mechanisms are saturable. Surprisingly, the α-factor secretion from MS71Hp and MS63Lp showed clear RER1 dependency like the case of S12Mp (compare RER1 RET1 and Δrer1 RET1). This result would indicate that RER1 is involved in the ER localization of not only Sec12p but also Sec71p and Sec63p. It also implies that the ER localization of these proteins is fulfilled, at least partly, by dynamic retrieval from the Golgi apparatus.
To rule out the possibility that the α-factor moiety might have affected the localization, we examined the behavior of Sec71p without the Mfα1 fusion. The HA-tagged Sec71p (3HA-Sec71p) was introduced on a multicopy plasmid into the wild-type and Δrer1 cells, and the ER localization was confirmed by immunofluorescence (not shown). The cells were then labeled with Tran35S-label and subjected to immunoprecipitation analysis. As shown in Fig. 4, a large portion (38%) of 3HA-Sec71p received α1→6 mannosyl linkages (cis-Golgi modification) during a 20-min pulse, but the acquisition of the α1→3 mannosyl linkages (medial-Golgi modification) was very little in the wild-type cells (1% at 0 min and 6% at 60 min). In the Δrer1 cells, by contrast, a significant fraction of 3HA-Sec71p (8% at 0 min and 17% at 60 min) underwent α1→3 mannosyl modification. These observations indicate that the authentic Sec71p indeed utilizes retrieval from the cis-Golgi for ER localization and that Rer1p is important for this process.
Rer1p Is a Common Limiting Factor Involved in ER Localization of Sec12p and Sec71p.
The seemingly saturable nature of ER localization of MS71Hp and MS63Lp suggests that limiting component(s) exist for this mechanism. To test the possibility that Rer1p may be such a component, we examined the effect of Rer1p overproduction on the size of halo produced by MS71Hp. To make a stable strain that overexpresses MS71Hp, we constructed a glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter-driven MS71H (GAP-MS71H) and integrated it at the leu2 locus of SNY9. This integrant (SKY30) was transformed with RER1-3HA on a single-copy (CEN) or multicopy (2 μ) plasmid and examined by the halo assay (Fig. 5 Upper). As is the case of the cells expressing MS71H from a multicopy plasmid, GAP-MS71H in SKY30 cells caused halo formation (see Vector). The expression of RER1–3HA from the plasmid made the halo significantly smaller. By quantitative analysis of the halo size, the amounts of secreted α-factor were calculated to be 78 ± 8% and 52 ± 5% of the vector controls for the single-copy and multicopy plasmids of RER1–3HA, respectively. Western blot analysis using the anti-HA antibody indicated that this effect was not due to the decrease of the expression of MS71Hp (data not shown). A similar result was also obtained for MS63Lp (not shown). Thus, the raised dosage of Rer1p apparently remedies the mislocalization of the overproduced fusion proteins.
We also examined the effect of the increase of Rer1p on the halo formation by DDDm, which is a fusion protein between Mfα1p and a type II vacuolar membrane protein, Dap2p. This fusion has been shown to be quickly transported to the vacuole in the presence or absence of Rer1p (16). In contrast to the case of MS71Hp, RER1–3HA increased the secretion of α-factor from the wild-type cells expressing DDDm (see Fig. 5 Lower). This may suggest that Rer1p has opposite effects on retrograde and anterograde protein transport.
If Rer1p is in fact a limiting component commonly required for the retrieval of Sec12p and Sec71p, competition may be observed when either of them is overproduced. We have recently reported that the transmembrane domain (TMD) of Sec12p is an RER1-dependent ER localization signal, and the transplantation of this domain makes Dap2p efficiently localized to the ER. So we examined the effects of the co-overproduction of Sec71p, Dap2p (DDD), and the Dap2p derivative (DSD) containing the TMD of Sec12p on the halo formation by the MS71Hp-overexpressing strain (SKY30). If the overproduction of any of these proteins competes for Rer1p with MS71Hp, the retrieval of MS71Hp would become less efficient, which will lead to an increase of α-factor secretion. This was indeed the case for Sec71p and DSD. The result of the quantitative analysis of halos is summarized in Table 2. The co-overexpression of Sec71p or DSD increased the α-factor secretion of SKY30 by 1.7- and 1.9-fold, respectively. DDD showed no effect in this experiment. Importantly, such a competitive effect was abolished when the RER1 gene was disrupted (Table 2). Furthermore, the competition was partly suppressed by the co-overexpression of Rer1p as well (not shown). These observations indicate that Rer1p is responsible for the dosage-dependent overflow of ER membrane proteins, which is consistent with the idea that it is Rer1p that is limiting in the retrieval of these ER membrane proteins.
RET1 Is Also Involved in ER Localization of Membrane Proteins Without the Dilysine Signal.
Evidence has been presented that components of coatomer (COP I) are required for the Golgi-to-ER retrieval of membrane proteins containing the dilysine motif (9, 10). RET1 encodes the yeast counterpart of α-COP, a subunit of COP I. A mutation of RET1 causes temperature-sensitive growth and mislocalization of Ste2-Wbp1 fusion protein, which harbors the dilysine signal, from the ER to the plasma membrane (9). We examined the effect of this ret1 mutation on the localization of our fusions, S12Mp, MS71Hp, and MS63Lp, which do not have any dilysine or related motifs at their cytoplasmic ends. To use our tools, we constructed a ret1–1 Δmfα1 Δmfα2 Δbar1 strain (SKY27) by crossing SKY7 (MATα Δrer1::LEU2 Δmfα1 Δmfα2 Δbar1) and EGY101 (MATa ret1–1). In the course of construction of this strain, we dissected 20 tetrads after mating and sporulation, and 13 of them gave rise to four viable spores at 23°C, including 9 Leu+ Ts− spores. This indicates that the Δrer1 ret1–1 double mutant is viable. However, the Δrer1 ret1–1 cells did not grow at 35°C, at which temperature either single mutant can grow (data not shown), indicating some synthetic effect of these mutations. The obtained ret1–1 cells (SKY27) were transformed with a single-copy or multicopy plasmid harboring S12Mp, MS71Hp, or MS63Lp. As shown in Fig. 3, S12Mp on a multicopy plasmid in ret1–1 formed a small but visible halo, suggesting that COP I is also involved in the retrieval of Sec12p. In the cases of MS71Hp and MS63Lp, the effect of the ret1–1 mutation was more obvious. Halos were observed even when they were expressed from a single-copy plasmid. On the multicopy plasmids, the dependency on RET1 is seen for all the fusions examined. This result might argue that the role of COP I in the Golgi-to-ER retrieval is not restricted to the proteins that contain the dilysine motif. It may be also worth mentioning that the dependency on Rer1p and Ret1p varies among the three fusions. In the cases of S12Mp and MS71Hp, the disruption of RER1 showed a more severe effect than ret1–1. With MS63Lp, on the other hand, the halo size in ret1–1 is comparable to or even larger than that in the RER1 knock out.
Retrieval from the Golgi apparatus is important for many ER proteins to attain their strict localization in the ER. Several distinct systems have been studied to date. We have shown that Rer1p is essential for the retrieval of Sec12p from the Golgi to the ER and that the TMD of Sec12p acts as a signal in this Rer1p-dependent mechanism (14, 16). Erd2p, which resides in the Golgi membrane, functions as the receptor of the HDEL signal and thus sends back HDEL-bearing proteins to the ER (3–5). Coatomer (COP I) binds to the dilysine signal on the cytoplasmic tail of membrane proteins and drives formation of COP I-coated vesicles, a retrograde carrier from the Golgi to the ER (8–10). Are these seemingly different mechanisms responsible only for retrieval of their specific cargos?
Taking advantage of the halo assay method, which we developed to detect mislocalization of α-factor fusion proteins (13), we have shown in this study that Mfα1p fusions with Sec71p (MS71Hp), Sec63p (MS63Lp), and Sec12p (S12Mp) are all mislocalized to the trans-Golgi in the Δrer1 mutant cells. The role of Rer1p has also been demonstrated for Sec71p itself by the analysis of oligosaccharide modification. Because these three membrane proteins have completely different topology and share no apparent sequence homology, this suggests that Rer1p is a component of general retrieval machinery utilized by a variety of membrane proteins.
During the analysis of these fusion proteins, we realized that the Rer1p-dependent retrieval appeared to be a saturable process. When MS71Hp and MS63Lp were overproduced, they were mislocalized even in the wild-type cells. Similar overflow was also observed when DSDm, a Dap2p/Sec12p chimera with Mfα1p fusion whose ER localization is strongly dependent on Rer1p, was overexpressed in the wild type (M.S., unpublished result). The mislocalization of MS71Hp and MS63Lp due to their overproduction is partially suppressed by the co-overproduction of Rer1p. This would argue that Rer1p is one of the limiting factors required for retrieval of these membrane proteins. This possibility was further supported by a competition experiment. When Sec71p or DSD without the Mfα1p peptides was highly expressed together with MS71Hp, the mislocalization of MS71Hp was significantly exacerbated. This can be interpreted as indicating that the capacity of the machinery to retrieve these membrane proteins is limited, and thus, the overflow of MS71Hp becomes even worse when another cargo is overproduced. Such exaggeration of mislocalization was abolished when the RER1 gene was knocked out. These observations led us to conclude that Rer1p is a limiting component of the cellular machinery commonly involved in retrieval of various ER membrane proteins.
There are several possibilities about the role of Rer1p in retrieval. It could be a receptor of retrieval signal(s) or may function as a chaperone during membrane protein sorting in the cis-Golgi. It is also conceivable that Rer1p blocks transport of ER membrane proteins to the later compartments of the Golgi apparatus. Whether Rer1p is recycled back to the ER together with cargos is an important question in this regard. Interestingly, Rer1p appears to exert opposite effects on retrograde and anterograde transport from the cis-Golgi. Although the overproduction of Rer1p suppresses the halo formation from MS71Hp, it clearly increases the size of halo produced from Dap2-Mfα1p (DDDm). On the contrary, the disruption of RER1 causes the halo from DDDm to become slightly smaller (see figure 4 of ref. 16). These observations might imply that Rer1p plays roles not only in retrieval of ER membrane proteins but also in accelerating forward transport of vacuolar and perhaps plasma membrane proteins. If so, a complex mechanism may be required for Rer1p to distinguish these two categories of proteins.
We have also shown that all three fusion proteins, MS71Hp, MS63Lp, and S12Mp, are more or less mislocalized to the trans-Golgi in ret1–1, a mutant of α-COP. The COP I complex is known to be required for the recognition of dilysine retrieval signals, but none of these fusion proteins harbors dilysine or related motifs. Then, do Rer1p and COP I cooperate on retrieval of these proteins? The combination of Δrer1 and ret1–1 mutations confers a synthetic growth defect to the cells, indicating their intimate relationship. Furthermore, we found that the overproduction of Rer1p failed to suppress the mislocalization of MS71Hp in the ret1–1 mutant (unpublished result). From these, we suggest that Rer1p and COP I function in the same pathway.
To compare with other retrieval signal systems, we constructed an Mfα1-Wbp1 fusion protein that harbors a typical dilysine signal. This protein was mislocalized in ret1–1 but not at all in the Δrer1 cells (unpublished observation). We have already shown that BiP, a soluble ER protein containing the HDEL signal, is not significantly missecreted in Δrer1 (13, 14). Probably these dilysine and HDEL signals are recognized by their specific receptors and directly packaged in the COP I vesicles. Our current model for ER protein sorting in the cis-Golgi is illustrated in Fig. 6. As far as we examined, the TMD of Sed4p also seems to act as an Rer1p-dependent signal (16). How Rer1p recognizes various types of membrane proteins would be the most important next question and awaits further biochemical experiments.
We are grateful to David Feldheim, Randy Schekman, and François Letourneur for strains and plasmids, and to the members of our laboratory for helpful comments on the manuscript. This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan, by a fund (Biodesign Project) from the Institute of Physical and Chemical Research of Japan (RIKEN), and by a Research Fellowship for Young Scientists (to K.S. and M.S.) from the Japan Society for the Promotion of Science.
While this manuscript was in a revise process, Boehm et al. (35) reported that another mutant allele of RET1 also mislocalized Sec12-invertase fusion proteins and that Rer1p recycled between the early Golgi and the ER in a COP I-dependent manner. These observations are consistent with our conclusions.
↵* To whom reprint requests should be addressed.
- endoplasmic reticulum;
- transmembrane domain
- Received September 19, 1996.
- Accepted July 1, 1997.
- Copyright © 1997, The National Academy of Sciences of the USA
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- Cosson P,
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- Nakano A,
- Brada D,
- Schekman R
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- d'Enfert C,
- Barlowe C,
- Nishikawa S,
- Nakano A,
- Schekman R
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- Nakano A
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- Sato M,
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- Feldheim D,
- Rothblatt J A,
- Schekman R
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- Deshaies R J,
- Schekman R
- Rothblatt J A,
- Deshaies R J,
- Sanders S L,
- Daum G,
- Schekman R
- Sadler I,
- Chiang A,
- Kurihara T,
- Rothblatt J A,
- Way J,
- Silver P
- Green N,
- Fang H,
- Walter P
- Brodsky J L,
- Schekman R
- Feldheim D,
- Schekman R
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- Ng D T W,
- Brown J D,
- Walter P
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- Sikorski R S,
- Hieter P
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- Ohya Y,
- Goebl M,
- Goodman L E,
- Petersen-Bjφrn S,
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- Sanger F,
- Nicklen S,
- Coulson A R
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- Nakano A
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- Nishikawa S,
- Umemoto N,
- Ohsumi Y,
- Nakano A,
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Be prepared for any and every entertaining situation with this Serving Cart. The piece features three open shelves, offering more than enough space for drinks, glasses and other bar accessories you may need. Also, this cart is castered, making it easy to roll it around your party to accommodate all of your guests.
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Generals win 20th game of the year with 75-64 win over FMCC
February 11, 2019
Herkimer, NY - In a hard fought battle for first place in the Mountain Valley Conference, the Herkimer Generals used a 22-9 run over the last 6 minutes to defeat Fulton-Montgomery 75-64 on Monday night in Herkimer, NY. The victory gave the Generals their 20th win of the season and improved their Mountain Valley Conference record to 10-1 to take over first place in the conference. It was also their 17th straight win on the year.
After grabbing a 10 point halftime lead at 40-30, Herkimer maintained that lead into the second half before the Raiders went a 15-3 run to take a 55-53 advantage with just over 6 minutes remaining before the Generals closed on their late run. Derrick Felder (Yonkers, NY) and Musa Pough (Brooklyn, NY) led Herkimer's attack with 15 points apiece. Keshaun Howard (Brooklyn, NY) came off the bench to score 12 points, which included 3 3 point field goals. Isaiah Stone (Baldwin, NY) added 11 points while Teshaun Beckles (Bronx, NY) added 9 points and a couple of big defensive plays during the decisive run that gave Herkimer the win. The Generals hit 9 3 point shots as a team and went 22-27 from the free throw line on the night.
Herkimer now goes on the road for four straight games in conference play, beginning with a trip to Glens Falls, NY to take on the SUNY Adirondack Timberwolves on Wednesday evening, February 13th. teh start time for that game is set for 5:00 PM.


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Welcome to the three hundredth and thirty-ninth in a series of examinations of comic book legends and whether they are true or false. This week, did Superman inspire the Vulcan nerve pinch? Was Mister Mind always intended to be a worm? And what's the story of the almost crossover between Quantum and Woody and Black Panther?
Click here for an archive of the previous three hundred and thirty-eight.
Let's begin!
COMIC LEGEND: When he was first created, Mister Mind was not yet a worm.
STATUS: True
In 1943's Captain Marvel Adventure #22, the Monster Society of Evil serial began...
In an unusually ambitious storyline, this serial went on for TWENTY-FIVE parts, spanning two years!
In the first story, the mysterious Mister Mind debuts, but he is only a voice...
It is not until Captain Marvel Adventures #26 that we meet Mister Mind, and he is a WORM!
Mister Mind went on to become one of the top Captain Marvel adversaries of all-time and has remained a popular villain to this day (he was a major bad guy in 52, for instance).
However. when he was introduced, Otto Binder and the folks at Fawcett had no idea WHAT he was going to be!
In a demonstration that he was a great comic book historian even before he was a great comic book writer, in 1964's Alter Ego #7, Roy Thomas had the following fascinating piece of information from Otto Binder himself...
Mr. Mind wasn't a worm, at least not for the first half dozen chapters [Binder is off by a couple of issues, but what's a couple of issues between friends? -BC}. The CMA (Captain Marvel Adventures) brain-trust composed of Wendell Crowley as editor, Charles Clarence Beck as artist, and myself as scripter, got our heads together to figure out just WHO or WHAT Mr. Mind should be, after I invented him as a diembodied voice.
We undoubtedly went thru a hundred concepts, until somebody (and, frankly, in those skull sessions, I have no idea who first thought of any particular gimmick)..somebody said, "Why not take the most unusual thing we can think of? Not the traditional human or galactic villain, nor robot, nor this this nor that of the routine masterminds, but just the goofiest of all things---maybe a worm!"
I vaguely recall that this was enthusiastically endorsed by us with much laughter and a tongue-in-cheek attitude, we had not idea that thing would become POPULAR!!?? We truly were amazed at the electrifying response...letters pouring in...and believe me, with a readership of over one million as we had in those days, the mail can become pretty imposing. A rousing consensus simply loved Mr. Mind! Why? We never figured it out. You figure it out, you researchers today into the mysterious hypnotic power that comic characters had on readers.
It reminds me of an old legend I did about how Scott Lobdell came up with the idea of Onslaught before he knew exactly WHAT Onslaught was!
Oh, those comic book writers, always living on the edge!


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Cognate in Musicology
Students enrolled in a doctoral program at CCM are expected to demonstrate intellectual breadth through the completion of a significant program of study in a secondary field (cognate).
The music history cognate will consist of three graduate-level MUHS topics courses or seminars (at least 9 semester hours at the 6000, 8000, or 9000 level). The student should consult with a musicology faculty member whose specialty is in an area of the student's interest. If the faculty member agrees, s/he will become the student's cognate advisor and will help the student draw up a suitable plan of study according to the following guidelines:
- At least one of the courses must be an 8000- or 9000-level course.
- Courses in ethnomusicology may be included if appropriate to the area of specialization
- The following courses may not be used for cognate hours:
- Any courses being used to fulfill the student's degree requirement in music history
- Any courses being used as substitution for a DMA document.
The cognate advisor will participate on the committee for the student's final oral examination.


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Supplemental Health Care is seeking a Med Surg RN traveler with recent experience to join one of the trusted acute care hospital facilities we have partnered with, in Rochester, NY.
We are interviewing qualified RN's now for ASAP start dates.
Assignment Info:
-Shift: Days and Nights Available
-Shift Length: 12 hours, 36 hours/week
-Assignment: 12 weeks with the option to extend
-Bonus - $1000 Completion Bonus upon completion of every assignment
Not licensed in NY? No problem! You get the license and we'll reimburse you for the cost of it.
Supplemental Health Care Advantages:
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Contact Information:
Taylor Kiehl
716-253-2506 (texting capable)
Registered Nurse
Medical Surgical
Reference #: P719002


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Kachurin, O.I.; Matvienko, N.M.; Chekhuta, V.G., Disproportionation of N-methylaniline, Russ. Chem. Rev., 1979, 45, 43-47. [all data]
Kachurin, O.I.; Matvienko, N.M.; Chekhuta, V.G., N-Methylated p-toluidines, Russ. Chem. Rev., 1980, 46, 64-67. [all data]
Matvienko, N.M.; Kachurin, O.I.; Chekhuta, V.G., Kinetics and equilibrium of the transalkylation reaction of N-methylarylamines, Russ. Chem. Rev., 1982, 48, 42-45. [all data]


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Flaming heck! Girls Aloud beauty Nadine Coyle has a burning desire to expand her business empire - and she can't wait for the sweet smell of success.
Not content with a Mexican restaurant, and properties from Eire to California, the 22-year-old is to set up a chain of scented candle shops in her adopted home town of LA.
The hot star waxed lyrical about her Stateside plans when we caught up with her backstage in Belfast.
Smelly candles have become a must-have in the homes of the rich and famous with the likes of Britney Spears and Prince queueing for months to get the latest mood-setting varieties.
Nadine told us: "I'm so excited about it. LA's the perfect place for it too as people love to get pampered.
"I've already got my brother-in-law to create the decor.
"He's an artist and does amazing canvases so it will be a proper family business - and really personal. I've got my mum to run it while I'm away working with the band.
"I'll also bring some candles to go in our dressing rooms to calm our nerves before the shows."
Get ready for some weird and wacky varieties though.
Shoppers could expect a Sexy Yes, Yes, Yes candle - a combination of strawberry and passion fruit which can get lovers in the mood for a little late night action.
Or perhaps a pink and sparkly Jump candle would perk you up to get you ready for a girls' night out.
Nadine told us: "LA is my home now because all my family are in the States.
"There's no way I'm quitting the band. The candle shop and my businesses are something else I love to do.
"I'm having such a laugh with the band. Where else can you get your hair and make-up done for free for hours on end, then you go on stage with a great bunch of girls and have a blast?"
Great to see the fire still burning!


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Creative loft space located in downtown Santa Monica, ideal for ideation/planning meetings, workshops and presentations, it features a central conference or presentation space, multiple breakout areas, an incredible private terrace and interior design that inspires creativity and collaboration.
-- High speed wifi (300/150 mbps)-- 3' x 4' dry erase boards (and markers) -- 44" monitor with Apple TV, HDMI, VGA and Thunderbolt connection -- Ceiling mounted high def projector with 5' x 10' screen area (*extra rental fee applies)
-- WiFi -- Complimentary water dispenser-- SMEG refrigerator for cold beverage storage as well as kitchen refrigerator-- Outdoor meeting/dining area-- Sonos Sound System-- Two bathrooms-- End of day trash removal/cleaning (extra fee may apply)-- Advanced furniture setup-- Furniture rental-- On-site manager-- Reserved parking spot (there are five options within 0-3 blocks at variable rate for other drivers)
*ADD-ONS (Additional fees apply)-- Projector/Screen-- Outdoor Rooftop Grill (Grill tools, propane)-- Stove/Kitchenware Usage-- Dishware/Glassware/Silverware-- Custom Setup (Large groups/classroom-presentation etc)
Our building lobby houses:
Sidecar Doughnuts & Coffee (20% off their entire menu with proof of booking)Mendocino FarmsBluestone Lane Coffee
Clients are welcome to arrange their own food and beverage or access our roster of local restaurants, bakeries, and caterers. Our spacious, professional grade kitchen can be stocked with anything your team needs to have a fun, productive visit.


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TBAP Octavia AP Academy rated 'good' by Ofsted
TBAP Octavia Alternative Provision (AP) Academy in Wisbech, Cambridgeshire, has been rated as 'Good' by Ofsted following an inspection in May.
Ofsted highlighted the quality of care learners receive from staff and leaders, ensuring all learners are motivated and looked after throughout the day.
The leadership's increase in school improvement was also highlighted, with specific reference to tightened monitoring systems and investment in staff development.
According to Ofsted, staff use effective strategies and skilful interventions to support learners' significant social, emotional and mental health needs. Ofsted also reports that learners appreciated the tenacity and patience of staff.
TBAP Octavia's results have improved dramatically since it became part of TBAP in 2016, with 89 per cent of Year 11 learners achieving five or more GCSEs or equivalent in 2018, compared to 40 per cent in 2016. This compares to the latest available national average for AP schools of 12.3 per cent. Accreditation was achieved by 100 per cent of learners and attendance has increased from 55 per cent in 2016 to over 80 per cent in 2018.
Nick Morley, head of school, said: "I am delighted with our Ofsted report. Our team goes above and beyond for all our learners. We are driven by an unflinching desire to find solutions for our learners and we never give up on them. Over the past three years, we have developed dedicated processes and a curriculum so every learner enjoys a memorable learning experience. This ensures learners can progress successfully to the next stage of their education."
Examples of development at Octavia AP Academy include the nurturing of good relationships with all local commissioning schools and partnerships with community multi-agencies including a local farm. Progress has also been made by the Academy's Educational Psychologist regarding the application of Trauma Informed Practice and Attachment Theory to identify and address behaviours such as emotional avoidance.
Nick Morley adds, "TBAP Octavia's future is very bright, we aim to be a centre of excellence as a Flagship AP Academy. We are working towards the Gold Inclusion Quality Mark (IQM) and have recently been awarded an LGBT+ Rainbow Flag award by the Kite Trust for outstanding and continued development. We also have plans to introduce the Duke of Edinburgh Award scheme for learners."
Seamus Oates, chief executive officer, TBAP Trust, said, "Octavia AP Academy is great example of a TBAP Academy delivering excellent outcomes. Well done to staff and learners at Octavia, who have made excellent progress over the last three years."


text: Mike, I really want to put the Job Template Attributes in a collection. I will change the name and drop "-collection". The contents of the collection is not strictly limited to IPP Job Template Attributes. Pete Peter Zehler Xerox Research Center Webster Email: Peter.Zehler at Xerox.com<mailto:Peter.Zehler at Xerox.com> Office: +1 (585) 265-8755 Mobile: +1 (585) 329-9508 FAX: +1 (585) 265-7441 US Mail: Peter Zehler Xerox Corp. 800 Phillips Rd. M/S 128-25E Webster NY, 14580-9701 From: Michael Sweet [mailto:msweet at apple.com] Sent: Tuesday, April 08, 2014 3:28 PM To: Zehler, Peter Cc: IPP at pwg.org Subject: Re: [IPP] Are these attributes what were agreed to for IPP Scan? Pete, My only beef with the proposed additions are the "destination-attribute-collection" attributes. We should just include Job Template attributes directly in the destination-uris and destination-uri-ready collections. Thus, it would just be: destination-uris destination-uri [number-or-retries] [retry-interval] [retry-time-out] [any Job Template attribute] destination-uri-ready destination-uri [destination-info] [destination-is-directory] [destination-name] [any Job Template attribute] This would be consistent with how other attributes (job-sheets-col, covers, etc.) deal with embedding Job Template attributes in collection attributes. If you really want to put the Job Template attributes in their own collection, call it "destination-attributes"; we never use "collection" spelled out in the name, and generally only add -col if there is already a non-collection form of the attribute (e.g. media and media-col, finishings and finishings-col, etc.) On Apr 8, 2014, at 3:07 PM, Zehler, Peter <Peter.Zehler at xerox.com<mailto:Peter.Zehler at xerox.com>> wrote: All, Before I put out the Stable version of the IPP Scan specification I wanted to check with you regarding the addition of the attributes below. Let me know if it does not capture what we agreed to at the IPP teleconference. I plan to publish it on Thursday. Pete Peter Zehler Xerox Research Center Webster Email: Peter.Zehler at Xerox.com<mailto:Peter.Zehler at Xerox.com> Office: +1 (585) 265-8755 Mobile: +1 (585) 329-9508 FAX: +1 (585) 265-7441 US Mail: Peter Zehler Xerox Corp. 800 Phillips Rd. M/S 128-25E Webster NY, 14580-9701 Added the following new member element to the Job Template Attribute "destination-uris" 220.127.116.11 destination-attribute-collection (1setOf collection) This OPTIONAL attribute allows the client to specify attributes to be applied to the method implied by the "destination". For example if the destination uri scheme is "ipp://" this attribute would contain Operation and Job Template Attributes to be used in the creation of the IPP Print Job. Added the following new IPP Scan Service Description Attribute 8.4.2 destination-uri-ready (1setOf collection) The OPTIONAL "destination-uri-ready" IPP Scan Service attribute lists the configured destinations. This does not limit the destinations that a user may specify. It is a list of administratively set destinations to assist the user in selecting an appropriate destination. IPP Scan Services that support Push Scan MAY support this attribute. 188.8.131.52 destination-info (text(MAX)) The OPTIONAL "destination-info" member attribute provides a human readable description of the destination. 184.108.40.206 destination-is-directory (boolean) The OPTIONAL "destination-is-directory" member attribute indicates if the destination is a folder that can contain multiple files. The default value if this attribute is not included is 'false'. 184.108.40.206 destination-name (name(MAX)) The OPTIONAL "destination-name" member attribute provides a human readable user friendly name for the destination. 22.214.171.124 destination-uri (uri) The REQUIRED "destination-uri" member attribute specifies the destination. 188.8.131.52 destination-attribute-collection (1setOf collection) This OPTIONAL "destination-attribute-collection" member attribute allows the administrator to specify attributes to be applied to the "destination-attribute-collection" Job Template Attribute (See section 220.127.116.11). _______________________________________________ ipp mailing list ipp at pwg.org<mailto:ipp at pwg.org> https://www.pwg.org/mailman/listinfo/ipp _________________________________________________________ Michael Sweet, Senior Printing System Engineer, PWG Chair -------------- next part -------------- An HTML attachment was scrubbed... URL: <http://www.pwg.org/pipermail/ipp/attachments/20140409/72f7881d/attachment.html>


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Newspapers and magazines publishers in Ondo State have honoured the National President of the Nigerian Medical Association (NMA), Prof. Adebayo Faduyile.
The Vice Chancellor, Obafemi Awolowo University (OAU) Ile-Ife, Prof Eyitope Ogunbodede, delivered a lecture titled: "Transforming the Nigerian Health Sector: The Critical Roles of All Stakeholders" in Akure.
Faduyile was conferred with the 'Ondo State Man of the Year 2018' by the Coalition of Newspaper Publishers in the state.
Ogunbodede lamented that three ingredients were lacking in the country.
He said: "We must redesign and implement our healthcare system at all levels such that it will be less about form and more about the work to be done.
"What we know does not seem to work anymore and healthcare system before us requires that we take a different approach and include all stakeholders in the rescue mission."
He noted that a value-oriented, performance-driven healthcare cannot be achieved without doctors and other healthcare workers adapting some traditions in medical practice.
Prof Ogunmodede added: "For health to be achieved in Nigeria and for us to make the required impact, there should be efficient primary health care services coupled with effective referral system."
Faduyile appreciated the publishers for the honour bestowed on him, promising to justify the confidence reposed in him.
The Chairman of the publishers, Oludaisi Adetarami, lamented that the health sector was facing a lot of challenges, especially low budgetary allocation.
He described Faduyile as a core medical professional whose talents were huge.


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If you're a big kid at heart and still have an enormous sweet tooth then you'll be more than a little bit happy to see that we have yet more retro sweets for you to enjoy - the Retro Sweet Chewbz!
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Purchased as a Christmas present for a sweetie loving friend. They were opened at 3.05pm on Christmas day, by 3.30pm only the wrappers remained and a friend with very 'furry' teeth emerged. I think they went down well!
I bought these sweets for my girlfriend as a stocking filler for Christmas. She loved them, taking her back to a golden age where the snow was fun and not a hinderance to our adult lives! The tube contains plenty of classics, just like nipping up to the local shop for a lucky bag when we were kids !
Wow wow wow kid in a candy store!! Just a shame i brought this for someone else and not for myself. It is jam packed with every retro sweet you can possibly think of from your childhood. Will be buying one or maybe two for myself!!!
when they arrived I was a little dissapointed with the amount of sweets .the picture used gives the impression that there is a greater quantity than there actually is. The quality of the sweets was fine was fine
Absolutely brilliant seeing the old brands of sweets
Bought this 'missile' full of goodies for my Good Lady Wife for Chrimbo, but after seeing all the sweets from my childhood there in front of me, I'm not sure I'll be able to wrap it without sampling one or two...
The tube is discribed as medium,but it looks half the size of the large. Great sweets but seeing as they are mostly 'penny' sweets I expected loads more for my money!
A blast from the past for kids aged 30 plus! Not as big as the picture, but still a nice gift for the money.
Firstly I couldn't believe how fast I received the retro sweet chewbz. There a present for my bro and I know he won't have a tooth left in his head after eating these. A great selection and a great pressie
I love the Albion bubblegum, takes me back to my youth.


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Delete Facebook Messenger Account
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Rivals Alexander and United battle for title
There is a lot riding on the final volleyball game of the regular season for the four UISD teams. For the fourth year in a row, rivals Alexander and United will battle for the District 29-6A title while LBJ and United South fight for third place.
No. 19 Alexander and United have either shared or won the district title outright since 2014. In 2013, Alexander, Del Rio and Eagle Pass were tied for the title. The same scenario played out last year when United lost at Alexander but beat the Lady Bulldogs at home for a share of the championship.
The Lady Bulldogs (27-10, 11-0 District 29-6A) have beaten United twice this season, once at the Lady Longhorn Tournament and again in the first round of district play where they overcame a 2-0 deficit and won 3-2 (21-25, 22-25, 25-21, 25-15, 15-8).
"Rivalry games always bring the best out in our kids. Every year is always a battle, and this year has been no different," Alexander head coach Ari Bermea-Mendoza said. "It should be a fun, competitive match and we are looking forward to it."
United (28-6, 10-1) has been solid since dropping that 3-2 heartbreaker. The Lady Longhorns are preparing to defend their home court as the Lady Bulldogs are looking to not share the title with anyone this year.
"This is one of the moments that we have been waiting to have, the final match of district play, and we're so excited to close at home," United head coach Lety Longoria said. "We have a lot of faith in our team, and I want for them to have fun on the court, play with the heart and the passion they have for the game and for their friends and family. I can't wait to be on the court with our girls as we play out an exciting and competitive rivalry game."
Rivals LBJ and United South also have riding a lot in their regular-season finale. Third place is on the line when United South travels to face the Lady Wolves at 7 p.m.
"It is always great to end district play at our house," LBJ head coach Selina Garcia said. "We have worked hard all season and fought hard each game, and we are going to continue to do just that. South is a great team and we know it's going to be a battle until the end. Our girls are focused and ready."
Whoever wins Tuesday night between LBJ and United South secures third place. A United South win guarantees the Lady Panthers third place. A United South loss and an Eagle Pass loss to Del Rio would put the Lady Panthers in fourth place. A United South loss and an Eagle Pass win would put them in a tie for the fourth and final spot, and a play-in game would be later on this week.
"We understand there's a lot at stake with this game," United South head coach Debbie Martinez said. "LBJ is a very good team and we're ready to take on this challenge. The girls are prepared and we're coming in with a lot of positive momentum. It's going to be a battlefield I'm sure the fans will enjoy."
Their first meeting was a four-set thriller that packed the stands. United South beat LBJ 3-1 (25-15, 16-25, 25-20, 25-21) as Ariana Guerra pounded 15 kills and Evelyn Cruz added 13 for the Lady Panthers.
LBJ (24-10, 6-5) took down Nixon 3-0 (25-18, 25-11, 25-17) on Saturday. United South (24-12, 5-6) is coming off a four-set loss to United, but the Lady Panthers put one solid set together where they showed their true potential.
Martin has a shot at the playoffs on the line as it hosts Alice at 6:30 p.m. while Cigarroa and Nixon already finished their seasons over the weekend. The Lday Tigers (17-7, 4-7 District 29-6A) need a win and a loss from Calallen (5-6) to force a play-in game after winning 3-0 at Calallen last week to split the series.
Harmony is playing for pride as it welcomes Cotulla at 6 p.m. to close out its first varsity season.
Twitter: @claralmtsports


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The West of England Academic Health Network invite you to the second Regional Polypharmacy event on Wednesday 12th June 2019 at Holiday Inn Bristol Filton.
We plan to look at what's been happening in the region since the last event in December 2018 and revisit the pledges that were made. We will discuss where polypharmacy fits into the national agenda and the long term plan, as well as sharing more information about how you can support your patients manage their own medicines.
Lastly we plan to showcase the newly developed dashboard which presents our local ePACT2 medicines optimisation and polypharmacy indicators and aim to use this to collectively agree the areas to focus on in the coming months and how we can continue to develop collaborative working across the region.
It's shaping up to be a full and interesting agenda. Places are limited so secure your place today.
Please email email@example.com to be put onto the circulation list for further updates


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|GitBox <...@apache.org>
|[GitHub] [zookeeper] mayawang commented on a change in pull request #995: ZOOKEEPER-3437: Improve sync throttling on a learner master
|Tue, 25 Jun 2019 21:45:51 GMT
mayawang commented on a change in pull request #995: ZOOKEEPER-3437: Improve sync throttling on a learner master URL: https://github.com/apache/zookeeper/pull/995#discussion_r297405316 ########## File path: zookeeper-server/src/main/java/org/apache/zookeeper/server/quorum/LearnerMaster.java ########## @@ -29,18 +31,81 @@ /** * interface for keeping Observers in sync */ -public interface LearnerMaster { +public abstract class LearnerMaster { + private static final Logger LOG = LoggerFactory.getLogger(LearnerMaster.class); + + // Throttle when there are too many concurrent snapshots being sent to observers + private static final String MAX_CONCURRENT_SNAPSYNCS = "zookeeper.leader.maxConcurrentSnapSyncs"; Review comment: Would this configuration be shared between leader and observer master? If so, I think there are a few assumptions that worth a closer look. 1) Leader and Followers are running on machines with similar capability (CPU, network bandwidth). This assumption is usually true. 2) ObserverMasters and Observers are also running on machines with similar capabilities (CPU, network bandwidth). This assumption may not be true. E.g. If we deploy some Observers in a different data center than the ObserverMaster, then the effective network bandwidth between ObserverMaster and Observer could be significantly smaller than the bandwidth between Leader and Followers. In this case, we might want to set a different maxConcurrentSnapSyncs for ObserverMaster than Leader. Do those assumptions make sense here? ---------------------------------------------------------------- This is an automated message from the Apache Git Service. To respond to the message, please log on to GitHub and use the URL above to go to the specific comment. For queries about this service, please contact Infrastructure at: email@example.com With regards, Apache Git Services
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text:
Anna Jordan's Bruntwood Prize-winning play, Yen explores a childhood lived without boundaries and the consequences of being forced to grow up on your own.
Hench is sixteen, Bobbie is thirteen. They're home alone in Feltham with their dog Taliban; playing PlayStation, streaming porn, watching the world go by.
Sometimes their mum Maggie visits, usually with empty pockets and empty promises. Then Jenny shows up.
Yen won the 2013 Bruntwood Prize for Playwriting and was first performed at the Royal Exchange Theatre, Manchester, before transferring to the Royal Court Theatre, London, in 2016.
This new edition published alongside the Royal Court Theatre production in January 2016.


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So here we go. A conflaguration of audible atrocity, sensual dismemberment and optical bewandering that was this years scumrun rally. Not to mention it was a f$&#@?# good larf!
It all started with a policeman taking a photo of a small graffitid motorbike in front of a not quite as small graffitid mx5 in my drive.
Thats right, we have had all kinds of run ins with the officiati of the various parishes we frequent across the isles of europe and to be sure it was not to be the last but this is the first time that ive had to utter the inevitable phrase of "hi there officer how can I help" before actually leaving my home!
Let me explain... Andy my co driver/conspirator/cameraguy and all round gaffer and I were in the midst of our final preparation the day before the hallowed scumrun route began, innocently inserting cameras in places where cameras should not be, laying more cable than the chinese telephone exchange and testing various items of need for the impending weekend when our local `bobby` took exception to andys mildly enthusiastic outburst of chavalry on our camera mounted mini moto.
We simply pointed out that we would never be using it anywhere near public roads of course, well not until we were out of his remit anyway... I quote him here for note "you can do what you like when you get on the ferry" so I dont think it too much to read from that comment that we are therefore fully supported by her majestys representatives in blue and in fact could be said to be emmisaries of this great country.
That was a turn up for the books...
A last word of note before I begin, for those who may be reading this as a Bugsplat Virgin (those two words should probably never be put together again) We are this years official camera crew for the event. I run the official Scumrun Youtube channel. Hence there may be a leaning to mention cameras and / or filming of various natures throughout this post.
The launch...
The next morning we headed off bright and early...
...the plan was to get there same time as the event team and film the arrival of the cars. Some naysayer with a tendency not to be able to drive in the wet (ah the subtle introduction of one of the themes of this years rally) caused a not insignificant delay to our arrival. Luckily we had left plenty of time and only arrived after 3 or 4 of the teams. Its fair to say that the weather had not gone favourably.
To say it was raining is like saying that the scumrun is a club for uk car enthusuasts to go on a sporting tour and have one or two draughts of ale...ie it was like the skies had decided to do all it could to make filming outdoors with expensive camera equipment a near impossibility.
Enter the gaffer (tape)
Now those that follow my adventures know I have a leaning towards fixing almost anything with the use of tbe greatest invention of all time,gaffer tape. Not the wheel, if your wheel was falling off what would keep it on! Gaffer! So anyway before I lose myself in the billion or so innovative applications of my favourite thing, one of these is taping a pair of water proof trousers over a sony video camera.
It has to be said that it worked well!
The teams started arriving in ernest and were fed through the scrutineering bay. We were subjected to a noise meter test by the stewards to which we unsurprisingly failed monumentally. We registered 103 decibels, which google reliably informs me is the same as a jet flyover at 1000 feet, heh heh
So we were not allowed on the track for the track day laps bit. Which was probably for the best given the combination of thruxton being a high speed track, the weather conditions making it slippery, my borderline rear tyres and my uncontrollable need to go sideways.
This did not stop a few teams going out including a black granada limo that really seemed to be pushing it. At least the smoking stinking clutch and brake combination on their return indicated so.
It was good to see some of the scumrun regulars and legends turning up. While not all were partaking in the rally these took to the track and showed that should they return then their cars were still "up for it".
We also got to see Tiff Needell throwing his M3 around a very wet track with some lucky (or not depending on your perspective) donors to the charity. I've often heard the phrase from people "I had it properly sideways" and you think 'yeah yeah how come your still here then' but this was proper 45 degree 80mph stuff Tiff was producing. Made some fantastic footage.
After a bit of food where Ironically over a plate of lasagne I was more interviewed by Tiff about the event than the other way round (yeah get me man i'm too cool for school) we went on a bit of a wander round shoving the camera in various people's faces, interviewing the event team themselves and getting some sound bytes from the myriad of lunatics that were taking part.
It was clear even at this point that there were two or three teams that were throwing themselves into the 'spirit' of it whole-heartedly! I shall mention the Virgin atlantic 'babes' and interchangeable captain, the Scumrun pirates and the High Rollers just to mention a few... It was the first (but dear god not the last) time we were to here "Why are we pirates? cos we AAAAARRRRRRR!!!" and "KIMONO BABY!"
These and others have given me so much great footage to edit, worth a thanks for their efforts!
I'll apologise here for lack of photos at various stages, I was carrying a massive camera at this point and don't have time to search round others for the moment, may add later on.
Parade lap...
Well here's the thing. Given we were massively too loud for the track, what do they do? send all 100 cars on the track at the same time with pretty much no briefing or rules (at least that had made it as far as us).
Everyone was very well behaved for, well, at least...100m or so. we were about 2/3 the way back and decided that to get some good footage we needed to head forth frontwards. I believe some teams saw our 'manoeuvres' and thought 'oh they are the camera crew they must be allowed' and some thought 'well if he's doing it, we can' - either way a safe but fun couple of laps were had by all!
After the official briefing and a bit of partying we all hopped in our cars and headed south to Portsmouth to get the ferry. Waiting for the ferry it was clear that this was going to be a good 'run. The teams were in full swing. One of the event BMWs had its number plate changed to "BUM FUN" with Ed and Jess obliviously having a 'touching' photo taken next to the car whilst 20 odd people were p£$%&%$ themselves watching. The Batmobile lit its Afterburner which was a fantastic spectacle, what was more amusing was when the car behind started to melt...only on scumrun.
We then boarded the ferry...scrapily (no not misspelt, SCRAPE - ILLY) as always, ramps and the bug dont mix.
So...So far we were doing well...all cars made it to the ferry (I believe) that in itself was an achievement compared to some previous years!
and...nobody had a bloody clue where we were going yet... The fun was only just starting!


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To the Editor:
"Media Tips and Gulf Money Fuel Campaign to Sabotage Qatar's Cup" (Sports pages, Feb. 2) implies that the challenges facing Qatar as host of FIFA's 2022 World Cup are because of the current Gulf crises. However, the challenges facing Qatar 2022 predate the crises.
When Qatar was awarded the right in 2010 to host the World Cup, it was assumed it would usher in a period of bridge building, peace, prosperity and moderation in the Middle East. It turned out that it was anything but in the interests of the region. FIFA, which has been marred with accusations of corruption, found itself being used as a vehicle to advance the political agenda of Qatar.
Qatar is using the tournament to whitewash its image of being a host to Islamist extremists. Yet it continues to host such Islamists as Yusuf al-Qaradawi, who is barred from the United States and Britain. He has openly endorsed suicide attacks against Israelis (later reversing himself) and continues to be an ideological inspiration for Qatar's regime. What Qatar laws and ethics dictate are in total contradiction to the FIFA Code of Ethics.
When FIFA decided to investigate allegations of corruption related to Qatar 2022, hopes were high that it would no longer tolerate corruption. Allegations of corruption that surfaced again in 2018 would tend to indicate that FIFA did an incomplete job.
Trust in global soccer as a bridge to peace and accountability is at stake. Before Qatar is allowed to host FIFA World Cup 2022, it must first answer questions about corruption allegations and address issues of anti-Semitism and extremism.
If 2022 comes and Qatar does indeed host the World Cup without changing, the message the world is giving to Muslim and Arab youth is that extremism is acceptable. Qatar should also not be allowed to hide behind the current Gulf crises to avoid answering questions related to FIFA World Cup 2022.
Ghanem Nuseibeh
The writer is the founder of Cornerstone Global Associates, which published a report critical of holding the 2022 World Cup in Qatar.


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Allied Carpet Cleaning Boise | Upholstery Cleaning Boise | Area Rug Cleaning Boise
Copyright 2019 All Rights Reserved
Entire House
Carpet Cleaning
Max 3000 Square Foot House
Add $25.00 for Steps
Carpet Cleaning Coupons
Meridian, Idaho Carpet Cleaner
If you live in Meridian, Idaho and are looking for a reliable, trustworthy carpet cleaner, Allied Carpet Care is your company. With over 30 years of cleaning carpets and upholstery in Meridian, no job is too big or too small. All of our carpet cleaning technicians are professionally trained and insured. All of our work comes with a 30 day guarantee and if for any reason you are not satisfied, we come back and reclean for free.
Sofa & Loveseat Cleaning
$25.00 per room
Carpet Cleaning
3 room minimum
Areas over 250 s/f considered 2 rooms
Additional Services Provided
Call us at (208) 939-3815 to Schedule
Allied Carpet Care


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Additional Information
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