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Not an emergency?
24hr Emergency plumbing & drain service No callout charges
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Welcome to Drain Doctor Croydon North Plumbing and Drainage.
Drain Doctor is the largest residential and commercial emergency plumbing & drainage service organisation in the UK.
We are committed to the highest standards of customer care, quality and professional service.
Every job is priced before work starts, with all our technicians working to a national pricing menu. No call out charges even for weekends or bank holidays.
Calling our free phone or local telephone number puts you in direct touch with our Service Centre. You receive the benefits of National standards with local services.
We always complete our jobs subject to availability of materials and our workmanship is guaranteed.
At Home
Whenever, wherever, or whatever your plumbing or drainage needs may be, Drain Doctor are ready to give you swift and professional service. We pride ourselves in being able to solve your plumbing or drainage problems quickly, efficiently, and with the service attitude that will make you glad you called us.
Why should Drain Doctor be your first choice?
- You can call out our technicans 24 hours a day, 7 days a week.
- Your telephone call will be answered by a human being, never an answerphone.
- You will pay no extra charges for service visits outside normal working hours.
- We will keep in constant touch with you until the job is done.
- You will never have to pay a call out charge.
- You see and agree the price before work is started.
- Your technician will be courteous, highly competent and in uniform.
- The Drain Doctor vans carry stock to complete most jobs then and there.
- All work areas will be left clean and tidy.
- We specialise in planned maintenance work.
At Work
Because our service combines expertise in both plumbing and drainage, our commercial accounts realise that they only have to make one call for all their needs. Often drainage problems can be allied to plumbing problems, so it is always good to have double expertise on hand.
Our clients also need a service company who will be on call and will come out when they need them. Not when they can fit you in! Business can be lost through delay. We pride ourselves in giving a fast local service and working to your time table.
You will always get a immediate response from a member of our Customer Service team, at what ever time you call, all year round.
"It's a great day at Drain Doctor Croydon North"
...we aim to give unrivalled levels of customer service.
If you have an emergency plumbing and drainage problem, ring Drain Doctor Croydon North on 0203 281 7400 and consider your problem solved.


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Introduction to Business Process Modeling, Analysis and Design
- day(s)Duration
- 21 CDU/2.1 CEU/21 PDU/Credits/Units
- MDP262BCourse Code
- This course is part of the Business Process Management Professional program that provides the practical tools and foundation used in analyzing business processes and redesigning or improving processes.
Course description
This course provides an introduction to the skills and techniques required to analyze and design new processes or to redesign and improve existing processes. This course focuses on identifying opportunities for process change and presents the process analysis, modeling and design techniques and tools required to improve performance. Participants will learn to scope specific processes using graphical models to document results. And, they will learn how to define measurement techniques for evaluating outcomes. They will also learn how to model As-Is and To-Be process workflows using BPMN notation. The course is organized around a universally applicable case study that will be developed by attendees working in teams. The approach is hands-on and business oriented and attendees will be able to immediately apply what they learn within their own organization.
Who should attend
This course is designed for business analysts and practitioners and provides a comprehensive introduction to the skills and techniques used in analyzing business processes and redesigning or improving processes. This Analysis and Design course is a must for business and IT architects, analysts and designers involved in process-managed organizations, process-based change, and the automation of process solutions. TQM, Lean and Six Sigma team members, requirements specialists, project managers, HR competency professionals and change agents will all benefit from the knowledge and skill acquired in this course.
MDP260 is a prerequisite for all courses in the BPM Curriculum.
What you will achieve
- Understand the principles and benefits of proven analysis and design techniques
- Understand how to identify opportunities for improving business process performance
- Understand the roles and responsibilities of the people in the process
- Understand how to develop a common language for describing business processes
What you will learn
- How to appropriately analyze and scope a business process problem
- How to create BPMN process flow diagrams
- How to select and apply the proper tools and techniques to model, analyze, manage, measure and improve processes
- How to apply creative thinking approaches and design techniques to process improvement
- How to develop critical measures for evaluating business process performance
- How to develop and apply testing, and validating methods to new process designs

subset: commoncrawl


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The ReptiHabitat™ Aquatic Turtle Kit is a great starter kit for new Aquatic Turtle keepers, or keeping a variety of turtle hatchlings. This kit has everything your need to get started!
Please contact us for current pricing and availability.
Animal House Pet Center is your one-stop location for all your pet needs. We are a full line pet store in the Tampa Bay Area, offering a wide array of pets, pet foods, supplies and health-care products. You are sure to find everything you need for your dog, cat, bird, reptile, fish and small animals at Animal House Pet Center.


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68 spreads = 136 pages rendered. The last 34 pages need to be digitised now. Then some correction, editing and the book will be ready for print :) My digital slr is not with me anymore so, forgive the quality of pictures.
Sunday, November 21, 2010
Render farm
Posted by somesh kumar at 7:00 AM
Labels: Going to school, renders
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Qualities that Makes Hussain al Nowais the Leading UAE Chairman
Successful companies have the most talented chairpersons. Both public and private institutions should invest in having the most capable chairman. The goal is to have a leader who will inspire more people to trade with the institution. Currently, among the best chairpersons in UAE is Hussain al Nowais. People seek to learn how Hussain al Nowais has acquired the outstanding reputation. The following are qualities that make Hussain al Nowais the leading UAE chairman.
The academic knowledge is the first qualities that have contributed to the success of Hussain al Nowais becoming the top UAE chairman. Quality education is essential if you desire to excel in life. All institutions will use the academic qualification as a measure when appointing their chairpersons. The idea is to work with people who have received the training to perform the tasks required by the organisations. The academic qualifications are therefore the prime factors causing Hussain al Nowais the leading UAE chairman. He has the educational expertise in directing the company to accomplish great things.
All the top chairpersons in UAE has a high level of expertise including Hussain al Nowais. Leadership is a learning process that takes time to grow the skills. Therefore, why organisations seek to have chairpersons with high levels of skills. The reason is that these individuals have encountered many issues over the course of their leadership. You can tell right leaders for not rushing to make unwise decisions and considering all essential factors before taking action. Many people recognise Hussain al Nowais as the top UAE chairman for having the high level of experience in both public and the private sector. Therefore, he knows the need for patience when interacting with various stakeholders to the company.
Hussain al Nowais is passionate about his job and delivering the best services to the clients. Through passion your job become more enjoyable which enhance the chances of success. As a company, you need to inspire people to enjoy working for you. Such individual have the energy to work for long hours towards the achievement of the organisational goals. If you want to know someone who loves his job you should seek more information about Hussain al Nowais. He is committed to offering his very best efforts when serving people. Hence, why Hussain al Nowais has become the top UAE chairman.
Many individuals look up to Hussain al Nowais for inspiration in their careers and personal lives. He is a symbol of excellence through hard work and passion. Therefore, why many individuals will name him as the best UAE chairman.


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This charming studio with designer touches is comfortable and cozy. Full kitchen with granite counters and cherry wood cabinets and upscale appliances. Completely stocked with dishes, cutlery, pots and pans, linens and towels for your use. The brand new queen-size bed is incredibly comfortable with Egyptian cotton linens. There is a full bath with shower and tub, in unit washer & dryer, wifi internet connection, TV and cable, and air conditioning. Enjoy alfresco dining on the lanai. The secure building has a large swimming pool and BBQ area. We also provide beach towels, beach chairs and lite weight cooler. No smoking please.
It's the perfect Honolulu hideaway, centrally located yet a short distance away from everything ! Located on the 6th floor of a quiet secure building on the slope of Punchbowl crater, site of the world recognized National Cemetery of the Pacific. FREE private parking, FREE internet and FREE laundry. It's perfect for the business traveler or couple looking for a peaceful and affordable place to relax. Enjoy the Honolulu views and if you're lucky enough to be sitting on your balcony on Friday night at 7:45 pm, you'll have a great view of the fireworks show put on by Waikiki hotels.
It's close to the world famous Ala Moana Beach Park and Shopping Center. Just a 5 minute drive to Waikiki or take advantage of our great public transportation system to get you anywhere on the island. Even if you are here for work, you couldn't ask for a nicer retreat to come home to. We are sorry but house rules do not allow less than a 10 day stay.
For security reasons we do not provide address until booking. Guests will be contacted with directions and arrangements made for personal greeting for check-in. Our Transit Accommodations Tax ID# is W03061728-01.
There is now a strict NO SMOKING policy just approved by the owners association and a majority of the buildings owners. It states that the building is now a non smoking property throughout. This includes inside each unit and on the lanais.


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US9037213B2 - Method and apparatus to estimate location and orientation of objects during magnetic resonance imaging - Google PatentsMethod and apparatus to estimate location and orientation of objects during magnetic resonance imaging Download PDF
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- Prior art keywords
- gradient
- tracking
- sensor
- coils
- activation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active, expires
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/02—Measuring direction or magnitude of magnetic fields or magnetic flux
- G01R33/028—Electrodynamic magnetometers
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
- G01R33/285—Invasive instruments, e.g. catheters or biopsy needles, specially adapted for tracking, guiding or visualization by NMR
- G01R33/287—Invasive instruments, e.g. catheters or biopsy needles, specially adapted for tracking, guiding or visualization by NMR involving active visualization of interventional instruments, e.g. using active tracking RF coils or coils for intentionally creating magnetic field inhomogeneities
The present application is a U.S. National Phase Application under 35 U.S.C. 371 of PCT International Application No. PCT/IB2009/050074, which has an international filing date of Jan. 8, 2009, and which claims priority from U.S. Provisional Patent Application No. 61/019,614, filed Jan. 8, 2008, and U.S. Provisional Patent Application No. 61/023,894, filed Jan. 28, 2008, the contents of which are incorporated herein by reference.
The present invention relates to methodology and apparatus to determine the location and orientation of an object, for example a medical device, located inside or outside a body, while the body is being scanned by magnetic resonance imaging (MRI). More specifically, the invention enables estimation of the location and orientation of various devices (e.g. catheters, surgery instruments, biopsy needles, etc.) by measuring electrical potentials induced by time-variable magnetic fields in a set of miniature coils.
Minimally invasive procedures: Minimally-invasive diagnostic or interventional procedures require either direct visual viewing or indirect imaging of the field of operation and determination of the location and orientation of the operational device. For example, laparoscopic interventions are controlled by direct viewing of the operational field with rigid endoscopes, while flexible endoscopes are commonly used for diagnostic and interventional procedures within the gastro-intestinal tract. Vascular catheters are manipulated and maneuvered by the operator, with real-time X-ray imaging to present the catheter location and orientation. Ultrasound imaging and new real-time MRI and CT scanners are used to guide diagnostic procedures (e.g. aspiration and biopsy) and therapeutic interventions (e.g. ablation, local drug delivery) with deep targets. While the previous examples provide either direct (optical) or indirect (imaging) view of the operation field and the device, another approach is based on remote sensing of the device with mechanical, optical or electromagnetic means to determine the location and orientation of the device inside the body.
Stereotaxis: Computer-assisted stereotaxis is a valuable technique for performing diagnostic and interventional procedures, most typically with the brain. The concept behind the technique is to have real-time measurement of the device location in the same coordinate system as an image of the field of operation. The current location of the device and its future path are presented in real-time on the image and provide the operator with feed-back to manipulate the device with minimal damage to the organs. During traditional stereotaxis, the patient wears a special halo-like headframe, which provides the common coordinate system, and CT or MRI scans are performed to create a three-dimensional computer image that provides the exact location of the target (e.g. tumour) in relation to the headframe. The device is mechanically attached to the frame and sensors provide its location in relation to the head frame. When this technique is used for biopsy or minimally-invasive surgery of the brain, it guides the surgeon in determining where to make a small hole in the skull to reach the target. Newer technology is the frameless technique, using a navigational wand without the headframe (e.g. Nitin Patel and David Sandeman, "A Simple Trajectory Guidance Device that Assists Freehand and Interactive Image Guided Biopsy of Small Deep Intracranial Targets", Comp Aid Surg 2:186-192, 1997). In this technique remote sensing system (e.g. light sources and sensors) provides the real-time location of the device with respect to the image coordinate system. Yet both the stereotaxis and the frameless techniques are typically limited to the use of rigid devices like needles or biopsy forceps since their adequate operation requires either mechanical attachments or line of sight between the light sources and the sensors.
Electromagnetic remote sensing: Newer remote sensing techniques are based on electromagnetism. For example, the Bladen and Anderson technique (WO 94/04938) is an active electromagnetic tracking methodology that requires the use of electromagnetic field generator or generators to determine the location and orientation of a sensor (Page 3 lines 17-36; Page 4 line 24 through Page 5 line 9). This methodology cannot be directly used during MRI because the application of an external electromagnetic field creates an unacceptable level of image artifacts. These artifacts can be avoided by interleaving the tracking step with the image acquisition step, which requires a modification of the MRI pulse sequences and lengthens the imaging time. It also requires mechanical modification of the scanner, to add the field generators into the structure of the scanner. The addition of conducting elements to the scanner (the coils of the generators) may result in substantial artifacts due to the creation of eddy currents and may create electromagnetic interference with the scanner. Acker et al (U.S. Pat. No. 5,558,091) disclose such a method and apparatus to determine the position and orientation of a device inside the body. This method uses magnetic fields generated by Helmholtz coils, and a set of orthogonal sensors to measure components of these fields and to determine the position and orientation from these measurements. The measurement of the magnetic field components is based on Hall effect and requires exciting currents in the sensors in order to generate the measured signals. The technique requires control of the external magnetic fields and either steady-state or oscillating fields, for the induced voltages to reach a state of equilibrium. These requirements prevent, or greatly complicate, the use of this technique with magnetic fields generated by the MRI system, and requires the addition of a dedicated set of coils to generate the required magnetic fields.
A different approach for remote sensing of location is disclosed by Pfeiler et al. (U.S. Pat. No. 5,042,486) and is further used by Ben-Haim for intra-body mapping (U.S. Pat. No. 5,391,199). Their technology is based on generating weak radio-frequency (RF) signals from three different transmitters, receiving the signals through an RF antenna inside the device, and calculating the distances from the transmitters, which define the spatial location of the device. As with the previous methodology, the application of the technology to MRI is problematic due to the simultaneous use of RF signals by the MR scanning. Potential difficulties are the heating of the receiving antenna in the device by the high amplitude excitation RF transmissions of the MRI scanner and artifacts in the MR image.
Dumoulin and colleagues disclose another approach to determine the location of a device, using a small receiving coil which is sensitive to near-neighborhood emitted RF signal during the MR imaging process (Dumoulin C L, Darro R D, Souza S P, "Magnetic resonance tracking", in Interventional MR, edited by Jolesz F A and Young I Y, Mosby, 1998; U.S. Pat. No. 5,318,025). Dumoulin and colleagues (U.S. Pat. No. 5,318,025) teach a tracking system based on induction of signals in receiving coils by the radio-frequency (RF) emission from the tissue near the device (Column 4, lines 33-50). It requires the use of all the components of an MRI scanner, including a high-intensity homogenous magnetic field, a set of three orthogonal magnetic gradient fields, RF transmission system, an RF receiving system, in addition to its own processing and controlling modules (FIG. 6 and Column 6, line 44 through Column 7, line 2). Since it is based on the same mechanism as that of image acquisition by MRI scanner, it can be viewed as imaging of a very small region of interest. Consequently, it requires the presence of material that can generate MR signals in the vicinity of the RF coils, either tissue (if the sensor is inside the body, like in catheters) or a small chamber of contrast agent (if tracking is done outside the body). Furthermore, the Dumoulin system requires substantial modification of the normal sequence of image acquisition to enable tracking (FIG. 4 and Column 4). This substantially complicates the programming of the pulse sequence of the scanner (due to the need to add RF and gradient activations for tracking that interfere with the imaging sequence), lengthens the time of scan, and limits the update rate of tracking. Additional major limitations of this tracking technique include the potential heating of the RF coils, especially inside the body; and the need to determine the orientation indirectly through the use of estimated location of at least 2 separate RF coils, with limited accuracy when small sensor must be used (i.e. when the distance between the two coils is short).
Interventional MRI: Many of the advantages of MRI that make it a powerful clinical imaging tool are also valuable during interventional procedures. The lack of ionizing radiation and the oblique and multi-planar imaging capabilities are particularly useful during invasive procedures. The absence of beam-hardening artifacts from bone allows complex approaches to anatomic regions that may be difficult or impossible with other imaging techniques such as conventional CT. Perhaps the greatest advantage of MRI is the superior soft-tissue contrast resolution, which allows early and sensitive detection of tissue changes during interventional procedures. Many experts now consider MRI to be one of the most powerful imaging techniques to guide interventional interstitial procedures, and in some cases even endovascular or endoluminal procedures (Yoshimi Anzai, Rex Hamilton, Shantanu Sinha, Antonio DeSalles, Keith Black, Robert Lufkin, "Interventional MRI for Head and Neck Cancer and Other Applications", Advances in Oncology, May 1995, Vol 11 No. 2). From the presented background on current methodologies, one can define the ideal system for minimal invasive procedures: It should provide real-time, 3-dimensional, non-ionizing imaging (like MRI or ultrasound) as feed-back to the user for optimal insertion and intervention; it should implement flexible, miniaturized devices which are remotely sensed to provide their location and orientation. By combining a composite image of the field of operation and the device location and orientation, the operator can navigate and manipulate the device without direct vision of the field of operation and the device. This may facilitate the use of minimal invasive intervention in the brain or other organs.
Motion artifacts in diagnostic MRI: Motion during scan hampers the image quality of MRI. The relative phase evolution of the MR signal induced as a result of the nuclear spin motion between the phase encode steps, presents as ghosting and smearing of signal intensity to incorrect locations in the image space (A. W. Anderson, J. C. Gore, "Analysis and correction of motion artifacts in diffusion weighted imaging," Magn. Reson. Med., vol. 32, pp. 379-387, 1994; R. J. Ordidge, J. A. Helpern, Z. X. Qing, R. A. Knight, and V. Nagesh, "Correction of motional artifacts in diffusion weighted MR images using navigator echoes," Magn. Reson. Med., vol. 12, pp. 455-460, 1994). Motion induced artifacts, specially the ghost and blurring artifacts, can significantly degrade the imaging sequences and also severely reduce the accuracy of functional MRI by deteriorating the weak activation signal. Retrospective motion correction techniques are currently used in routine MRI applications (M. Jenkinson, P. Bannister, J. M. Brady, S. M. Smith, "Improved optimization for the robust and accurate linear registration and motion correction of brain images," NeuroImage, vol. 17, pp. 825-841, 2002; D. Atkinson, D. L. G. Hill, P. N. R. Stoyle, P. E. Summers, S. Clare, R. Bowtell, and S. F. Keevil, "Automatic compensation of motion artifacts in MRI," Magn. Reson. Med., vol. 41, pp. 163-170, 1999; B. Kim, J. L. Boes, P. H. Bland, T. L. Chenevert, C. R. Meyer, "Motion correction in fMRI via registration of individual slices into an anatomical volume," Magn. Reson. Med., vol. 41, pp. 964-972, 1999; V. L. Morgan, D. R. Pickens, S. L. Hartmann, R. R. Price, "Comparison of functional MRI image realignment tools using a computer-generated phantom," Magn. Reson. Med., vol. 46, pp. 510-514, 2001; L. Freire, and J. F. Mangin, "Motion correction algorithms may create spurious brain activations in the absence of subject motion," Neuroimage, vol. 14, pp. 709-722, 2001). The realtime tracking of motion, for example by a tracking sensor that is attached to the moving organ, can be used prospectively during the scan to eliminate, or reduce, the effect of motion on the image quality.
MRI Pulse Sequence
A pulse sequence is a pre-selected set of defined radiofrequency (RF) and gradient pulses, usually repeated many times during a scan, wherein the time interval between pulses and the amplitude and shape of the gradient waveforms will control the MR signal reception and affect the characteristics of the MR images. Pulse sequences are specialized computer programs that control all hardware aspects of the MRI scan. Specific pulse sequences have been created to achieve various contrast mechanisms in the obtained images, and they are typically dependent on the magnetic field strength, the characteristics of the gradient fields, the characteristics of the RF fields, the type of imaging coil used, the clinical application of the sequence, and more.
Gradient Based Tracking
The EndoScout tracking system (Robin Medical, Baltimore, Md.) was developed to assist MRI-guided interventions in accordance with methods disclosed in U.S. Pat. No. 6,516,213, incorporated herein by reference in its entirety. The tracking is based on the gradient fields of the scanner. The system passively "listens" to the gradient activations and can track any device without the need to generate reference fields (as done by virtually all other electromagnetic tracking technologies). The EndoScout provides in real-time the position (location and orientation) of the device during MR scanning. This data enables the users to guide an interventional procedure to a target inside the body, using a minimally invasive approach. Devices and tools to be tracked may include: diagnostic devices (e.g. biopsy needles, aspiration needles), therapeutic devices (including devices used to destroy tissue, like RF ablation, laser ablation or cryo-therapy probes, or devices used to deliver therapeutic agents like drugs, chemotherapy agents, genetic vectors, stem cells, etc.) and various support tools (e.g. suction tips, pointers).
The main advantage of the gradient-based tracking technology is its independence of scanner type and mode of operation. However, some features of the pulse sequences may have adverse effects on the tracking performance and may result in less accurate or non-stable tracking. For example, the creation of 2-dimensional images requires encoding in two directions, which is achieved by frequency encoding and by phase encoding. The phase encoding typically involves gradient activations with variable amplitudes, beginning with a high positive amplitude, progressing through a low level and zero level amplitude, and ending with a high negative amplitude. During the time when gradient activations go through the low amplitude range, the tracking may be less accurate and create fluctuations in the tracking results that are in phase with the phase encoding of the scanning process. To eliminate this inaccuracy, the sampling window of the tracking system can be enlarged. To achieve maximal accuracy and stability, the sampling interval should include the whole length of the phase encoding cycle. While this can eliminate the accuracy problem, it may also result in low dynamic response of the tracking system, as typically large sampling windows are used, and the tracking results may be inaccurate if the tracked object is moving.
Another potential problem is the existence of eddy currents, which are induced when the gradient fields are changed over time, i.e. during activation and deactivation. These eddy currents are a well known phenomenon in MRI technology and are accounted for by the imaging process. The EndoScout tracking system uses maps that describe the spatial distribution of the gradient magnetic fields in each scanner where the system is used. These gradient field maps are measured during installation of the system. Eddy currents constitute a complex non-linear phenomenon that may depend on the specific gradient activation pattern in each moment, so eddy currents that affect the gradient field during mapping may differ from eddy currents that affect the gradient field during scan. Thus it may be difficult or even impossible to account for all types of eddy currents that are generated by various gradient combinations that are used during a scan.
An object of the present invention is to provide a novel method and apparatus for determining the instantaneous location and orientation of an object moving through a three-dimensional space, which method and apparatus have advantages in one or more of the above respects.
Another object of the present invention is to provide such a method and apparatus which is particularly useful in MRI systems by making use of a basic universal component of the MRI system, namely the time-varying magnetic gradients which are typically generated by a set of three orthogonal electromagnetic coils in such systems.
Another object of the present invention is to provide such a method and apparatus which is particularly useful in MRI systems by modifying the time pattern of gradient coil activations during scan to provide more data for the tracking system.
According to one aspect of the present invention, there is provided a method of determining the instantaneous location and orientation of an object moving through a three-dimensional space within the imaging space of a magnetic resonance imaging apparatus during operation of said magnetic resonance imaging apparatus. The method includes applying to the object a sensor for measurement of an instantaneous magnetic field within the imaging space of a magnetic resonance imaging apparatus, activating gradient coils of the magnetic resonance imaging apparatus during scanning operation of the magnetic resonance imaging apparatus so as to produce a magnetic field used for imaging, producing a tracking mode gradient activation signal at least one additional time point, the produced tracking mode gradient activation signal having command parameters for tracking of the object, resulting in a tracking mode activation of the gradient coils, measuring the instantaneous values of magnetic fields which are generated by the tracking mode activation of the gradient coils of the magnetic resonance imaging apparatus, identifying the command parameters of the tracking mode gradient activation signal, and processing the measured instantaneous values of the magnetic fields generated by the tracking mode activation, together with the identified command parameters, to compute the instantaneous location and orientation of the object within the space.
According to further features in embodiments of the present invention, the production of the tracking mode gradient activation signal may include bi-modally activating the gradient coils of the magnetic resonance imaging apparatus, wherein the bi-modal activation has a zero net effect on the imaging. According to additional features, identifying of the command parameters may include measuring of the command parameters, or retrieving stored information on the command parameters. The command parameters may include amplitude and rate of change of current flow of the tracking mode gradient activation signal.
According to further features in embodiments of the present invention, the method may further include measuring of instantaneous values of magnetic fields generated during the scanning operation and processing the measured instantaneous values of magnetic fields generated during the scanning operation together with the measured instantaneous values of the magnetic fields generated by the tracking mode gradient coil activation, to compute the instantaneous location and orientation of the object within the space.
According to further features in embodiments of the present invention, production of the tracking mode gradient activation signal may be done during a quiet section of a cycle of the scanning operation, during non-imaging time periods, resulting in a longer overall scan time; by adding activations of all three gradients for each phase encode step; by adding a single activation of a gradient for each phase encode step and toggling between the three gradients; or may be done only for gradient or gradients that use a gradient activation sequence with a variable amplitude in time for scanning. In some embodiments, a trigger signal may be used to define the time of production of the tracking mode gradient activation signal.
According to further features in embodiments of the present invention, the sensor for measurement of an instantaneous magnetic field within the imaging space of magnetic resonance imaging apparatus may comprise a coil assembly including a plurality of sensor coils having axes of known orientation with respect to each other and including components in the three orthogonal planes.
According to yet further features in embodiments of the present invention, the sensor may include sensor coils oriented in a known orientation, and the processing may include storing in memory reference magnetic field maps of each of the three gradient coils of the magnetic resonance imaging apparatus for the imaging space of the magnetic resonance imaging apparatus, and simultaneously estimating the location and the orientation of the sensor by processing the measured instantaneous values of the magnetic fields generated by the tracking mode gradient coils activation together with the known reference magnetic field maps of the gradient coils and the known relative orientation of the sensor coils.
According to yet further features, the processing may be effected by an iterative optimization process. This process may be effected in real time to determine the instantaneous location and orientation of the object in real time. In some embodiments, the produced tracking mode gradient activation signal is further used to determine position with an update rate faster than the rate of motion of the scanned object and use this position information to eliminate motion artifacts from the scanned images. The method may be used for diagnostic or interventional MRI, for example.
According to another aspect of the present invention, there is provided a system for determining the instantaneous location and orientation of an object moving through a three-dimensional space within the imaging space of a magnetic resonance imaging apparatus during operation of said magnetic resonance imaging apparatus. The system includes a magnetic resonance imaging apparatus having a set of activating gradient coils, and a gradient activation control unit for controlling activation of said activating gradient coils, a tracking portion including a sensor placed on the object for measurement of an instantaneous magnetic field within an imaging space of the magnetic resonance imaging apparatus, a tracking control unit for producing a tracking mode gradient activation signal and for identifying command parameters of the tracking mode gradient activation signal, and an electronic interface connecting the sensor and the tracking control unit. The system further includes a processor including means for producing a gradient activation scanning signal, and means for processing measured instantaneous values of the magnetic fields generated by the tracking mode activation of the gradient coils, together with the identified command parameters, to compute the instantaneous location and orientation of the object within the space.
According to further features in embodiments of the present invention, the sensor may include a coil assembly including at least three sensor coils oriented orthogonally with respect to each other. In some embodiments, the coil assembly may include three pairs of sensor coils, in which one sensor coil in each pair has the same orientation as the other sensor coil in the respective pair, and in which each pair of sensor coils has a different orientation from the other pairs of sensor coils. In some embodiments, each sensor includes a pair of sensor coils, wherein a first sensor coil in the pair is parallel to, but laterally spaced from, a second sensor coil of the pair. In some embodiments, the sensor includes a coil assembly including a cylindrical sensor coil and two pairs of sensor coils positioned orthogonally with respect to the cylindrical sensor coil. In some embodiments, the two pairs of sensor coils are curved and in a saddle relation to the cylindrical sensor coil. In some embodiments, the two sensors in a pair of sensor coils are planar. The sensor may be active sensor, such as a Hall-effect sensor, a passive sensor such as a coil sensor, or any other suitable sensor. In some embodiments, the object may be a medical instrument moving in the body of a person for medical diagnostic or treatment purposes.
According to further features in yet additional embodiments of the present invention, the system may further include a triggering mechanism for triggering of the tracking mode gradient activation signal. In some embodiments, the tracking mode gradient activation signal is a bi-modal signal.
The disclosed methodology and apparatus enable the estimation of the location and orientation of an object or a device by using a set of miniature, preferably (but not necessarily) orthogonal coils. The simplest embodiment has a set of three orthogonal coils. However more complex coil sets, for example a set of three orthogonal pairs of parallel coils, can improve the accuracy of the tracking with a higher cost of the system. To simplify the presentation, the following disclosure deals specifically with a set of three orthogonal coils, and also refers to the more complex configuration of three orthogonal pairs of coils. However the same concepts can be applied to various combinations of coils by anyone familiar with the field of the invention.
The time change of magnetic flux through a coil induces electromotive force (i.e. electric potential) across the coil (Faraday Law of induction). MRI scanners generate time-variable magnetic fields to create magnetic gradients in the scanned volume. By measuring the induced electric potentials in the three orthogonal coils (or pairs of coils), and by getting the time pattern of the generated magnetic gradients as input from the MRI scanner, both the location and orientation of the device can be estimated.
The present invention has significant advantages over existing methodologies. Compared with stereotaxis, either the frame or frameless techniques, the new methodology enables the use of devices like catheters or surgical instrumentation without the need for direct line of sight with the device and under realtime MRI. Unlike the remote electromagnetic localization methodology of Acker et al the present invention is based on measurement of voltages induced by a set of time-varying electromagnetic gradient fields in a set of coils (Faraday Law), rather than the need to use activations of homogenous electromagnetic fields and gradient electromagnetic fields which induce voltages in a set of miniature conductors carrying electrical current (Hall effect). Thus, the present invention is totally passive, it does not require any excitation of the sensors, and the requirement for time-variable magnetic fields may be satisfied with controlling the activation of the gradient coils through the pulse sequence of the scanner. The methods disclosed by Pfeiler et al and Dumoulin et al require the use of two sensors to measure orientations and thus have limited accuracy of orientation estimation, while the present invention uses a sensor which provides simultaneously accurate orientation and location tracking. Unlike existing optical tracking systems, there is no limitation on the number of sensors being used, and there is no need to maintain a line of sight between the sensor and the tracking apparatus. All other tracking methodologies are based on their own reference system, and should be aligned with the MRI coordinate system by a time-consuming registration procedure. The disclosed tracking methodology does not require registration since it uses the same set of gradient coils which are used by the MRI scanner for spatial encoding of the images.
Tracking based on the gradient fields of magnetic resonance imaging (MRI) scanners is disclosed in U.S. Pat. No. 6,516,213 by Nevo. This technology is based on passive operation of the tracking system without any change of the scanner's hardware or mode of operation. To achieve better tracking performance, a technique to create a custom MRI pulse sequence is disclosed. Through this technique any standard pulse sequence of the scanner can be modified to include gradient activations specifically designated for tracking. These tracking gradient activations are added in a way that does not affect the image quality of the native sequence. The scan time may remain the same as with the native sequence or longer due to the additional gradient activations. The tracking system itself can use all the gradient activations (gradient activations for imaging and gradient activations for tracking) or eliminate some of the gradients and lock onto the specific gradient activations that are added to the custom pulse sequence. The advantages of the proposed technique are: better tracking accuracy, better dynamic response of the tracking system, and lower sensitivity to eddy currents that are generated during the scan and modify the gradient fields of the scanner.
Reference is now made to
Embodiments of the Tracking Apparatus
Reference is now made to
The electronic interface 12 may include a set of amplifiers 122 to amplify the low-voltage potentials which are induced in a sensor that has induction coils (from millivolts level to volts), a set of low-pass filters 124 to eliminate the high frequency voltage potentials which are induced by the RF transmission, having frequency range of 10-400 MHz (depending on the MRI magnet strength), and stop-band or notch filters 126 to remove various potentials induced by various components of the MRI scanner, for example the cooling system of the superconducting main magnet or the step-wise increase of the MRI gradients, which in a General Electric MRI scanner produce a 128 KHz artifact in the tracking sensors. Various commercial systems with programmable amplifier\filter combinations can be used to amplify and filter the low-voltage signals from the sensors. For example, a cascade of commercial amplifiers (e.g. PGA103 programmable gain operational amplifier and PGA205 programmable gain instrumentation amplifier by BURR-BROWN) may be used to condition the sensor signals.
Tracking processor and control unit 10 can be developed using readily available commercial hardware. For example, the measured signals from the sensor can be digitized by analog-to-digital (A/D) converter 102 using a standard data acquisition board (e.g. National Instruments, Austin, Tex.), and processed in real-time by a modern, high performance processor 104 (e.g. a Pentium processor by Intel Corporation). Another potential solution, which provides faster estimation rates, can be based on digital signal processor (DSP) boards, having built-in or attached A/D converter having at least 6 channels (3 coil signals and 3 MRI gradient signals), high-performance DSP for iterative solution of location and orientation, sufficient memory capacity for the program and for data (e.g. the reference magnetic fields), and communication bus for interface with the host computer or directly with the MRI scanner (e.g. Blacktip-CPCI processing board and BITSI-DAQ analog input/output adapter, Bittware Research Systems, Concord, N.H.). The software for the DSP or the PC processor can be developed with standard programming languages, for example C++ or assembly. In some embodiments, Matlab software development environment (The Math Works, Natick, Mass.) may be used to rapidly implement the estimation process as described above, followed by translation of the program into C-language to achieve faster program execution.
The interface with the MRI can have various configurations. In the disclosure of U.S. Pat. No. 6,516,213 the interface includes two main components—a channel to transfer the real-time location and orientation of the sensor, and a channel (or channels) to transfer the activation pattern of the gradient coils from the MRI scanner to the processing module. Either digital communication channels, analog channels, or a combination of the two can be used. With the Signa MRI scanner (GE Healthcare), for example, the gradient activation sequence is available as standard analog output from the gradient control system, and tracking information can be received by the MRI scanner through a standard serial communication or Ethernet line. In another embodiment an additional channel is used to acquire the triggering signal from the scanner, which is typically available through standard output connections on the scanner. When repetitive gradient activations for tracking are used, a third configuration can be used, in which only the trigger signal is needed to be acquired from the scanner's hardware, for example with an optically isolated line or through digital communication.
Reference is now made to
Tracking processor and control unit 10 uses the measured instantaneous values of the magnetic fields generated by the tracking mode gradient coil activation, together with identified command parameters 481, to compute the instantaneous location and orientation of the object within the space. This computation may be done as follows.
The sensor position may be estimated (step 240) by tracking processor and control unit 10 via an iterative process. First, an initial estimate is made (step 241) of predicted electrical potentials that are calculated from the known gradient field amplitude, the geometry of the sensor and its coils, and current values of sensor position variables. The measured electrical potentials are compared (step 242) with the estimated values. If convergence criteria are achieved (step 244), the resulting position variables (location and orientation) are transferred (step 246) to tracking output 47. If convergence is not achieved, the position variables are updated (step 248) and the process is repeated. In this way, improved values may be achieved in consecutive iterations of the process.
The estimated location and orientation may be further processed to improve the quality of the tracking, e.g. by the application of a low-pass digital filter on the estimations at a specific time, using previous estimations, and may be transformed into a data format which is required by the MRI scanner.
In some embodiments, the instantaneous magnetic fields generated by normal scanning are also used in the tracking calculation.
The exact implementation of the invented methodology depends on the MRI mode of imaging, and the following description relates to any standard or modified MRI sequence. During MRI scan, repeated generation of magnetic fields by the three gradient coils provides the spatial encoding of the received MR echo and enables the reconstruction of the image. A sample sequence is given in
As described in U.S. Pat. No. 6,516,213 to Nevo, the gradient coil activations may by themselves provide the required data to estimate the location and orientation of the sensor. The estimation process is based on minimization of the difference between measured and predicted sensor signals. This can be done by various minimization methods, for example the minimization of the sum of squares of the differences between the measured and predicted signals (the least squares method). The measured signals in the sensor coils are linearly related to the time derivative of the magnetic flux through each coil (Faraday Law of Induction). Thus the measured signals can be compared with reference signals that are calculated from the known distribution of the gradient fields in the scanner, the known pattern of gradient activation, and the known geometry of the tracking sensor, as detailed below.
The gradient activation control unit 48 provides the tracking processor and control unit (10) with the activation commands of the three gradient coils which generate the three magnetic gradient fields Gx, Gy, Gz in the scanner. A full description of the magnetic field as function of time and location with any mode of operation (G(t,x,y,z)) can be calculated within the tracking processor and control unit (10) by vectorial summation of the three time-variable magnetic fields of the three gradient coils and the time-invariant main field (Bo) of the MRI scanner (in the following description vectors are underlined, to distinguish from scalars):
G (t,x,y,z)= Gx (t,x,y,z)+ Gy (t,x,y,z)+ Gz (t,x,y,z)+ Bo (x,y,z) (1)
where x,y,z are coordinates along the three axes of the MRI coordinate system (X, Y, Z, respectively) and t is a time variable. Additional magnetic fields, which are generated by the RF (radio frequency) coils of the MRI, are not used for the method of estimating location and orientation of the current invention. These fields, which in clinical scanners alternate in the range of tens of mega-hertz, induce high-frequency electrical potentials in the sensing coils which can be removed by low-pass filtration.
The magnetic field that is generated during the activation of the gradient coils of the scanner can be calculated from the gradient field maps that are generated by simulation or are measured during the installation of the tracking system by using standard methods to measure magnetic fields. These maps can be stored in various formats, for example as a set of values for a 3-dimensional grid, or as 3-dimensional functions of locations X,Y,Z in the MRI imaging volume. The command signal of the gradient coil represents the time change of the amplitude of the gradient, so the gradient field as a function of time and location G(t,x,y,z) can be represented by multiplication of the gradient field by the normalized gradient command signal.
In one embodiment, as shown in
V=−dΘ/dt (2)
The magnetic flux through each coil in the sensor is determined by the magnetic field amplitude at the location of the coil, denoted by G(t,x,y,z), the coil area (A), and the angle between the gradient field vector and the orientation of the coil, as defined by a unit direction vector n vertical to the plane of the coil:
Θ(t,x,y,z)= G (t,x,y,z)· nA (3)
where the · denotes a vectorial dot product. The typical coil has multiple wire turns to increase its inductivity, so the area A represents the total induction area of the coil.
By using equations 1-3, one can predict the induced electrical potential Vp in each coil from the time derivative of the magnetic field:
Vp=−d[( Gx (t,x,y,z)+ Gy (t,x,y,z)+ Gz (t,x,y,z)+ Bo (x,y,z))XnA]/dt (4)
If the sensor does not move or rotate, the Bo field and the direction vector n are constant and the predicted electrical potential in the coil is given by:
Vp=−d[( Gx (t,x,y,z)+ Gy (t,x,y,z)+ Gz (t,x,y,z))]/dtXnA (5)
The Iterative Estimation of Location and Orientation
In order to predict the induced potential in each coil, the location and orientation of the coil should be given. Thus, when the estimation process is started, an initial guess of the location and orientation of the sensor is given by three position variables (e.g. the sensor coordinates in the Cartesian coordinate system of the scanner xs,ys,zs) and three orientation variables (e.g. the three Euler angles {Ψ, Φ, θ}). The position of coil n is given in the local Cartesian coordinate system of the sensor IJK as 3 coordinates in,jn,kn.
The position of coil n in the MRI coordinate system {xn,yn,zn} is given by vector summation of the vector location of the sensor in the XYZ coordinate system {xs,ys,zs} and the vector location of the coil in the IJK coordinate system {in,jn,kn} after transformation to the MRI coordinate system by the transformation matrix R:
The transformation matrix R is derived from the Euler angles of the sensor orientation:
The orientation of the coil in the MRI coordinate system is given by a transformation of the coil direction vector from the sensor coordinate system to the MRI coordinate system:
n XYZ =R·n IJK (7)
Once the location and orientation of the coil in the coordinate system of the MRI is determined, the predicted electrical potential on the coil can be calculated by equation 5. The actual electrical potential that is induced in the coil may be amplified by the signal conditioning system, so appropriate calibration is applied on the measured signals to yield the level of the measured electrical potential Vm.
The differences between the measured and predicted electrical potentials on all the sensor coils during the activation of the three gradient fields are used to calculate the cost function. For example, if gradient activation for tracking is used, the three gradient coils can be activated separately, and for each gradient activation, three pairs of predicted\measured induced coil potentials are determined. New values for the sensor location and orientation can be calculated by using a well established minimization procedure (e.g. the Levenberg-Marquardt search algorithm).
In the description above the cost function is based on nine different measurements (three sensor coils during the activation of three different gradient fields) and can be used to estimate the six unknown location and orientation parameters. When a six-coil sensor is used, the cost function is based on eighteen different measurements, which can improve the tracking accuracy by reducing the effect of noise. When the iterative process achieves the correct location and orientation of the sensor, the differences between the measured and predicted potentials will become small and the cost function will reach its minimal level (it will not reach the zero level due to various inaccuracies—for example inaccuracy in the geometry of the sensor, noise in the measured signals, inaccuracy in the gradient field maps, inaccuracy in the calibration of the signal conditioning system, etc.). The iterative process is stopped when the cost function achieves a small enough value, or the level of reduction of the cost function becomes too small, and the final set of coordinates is transferred from the tracking system to the MRI scanner as an updated location of the tracking sensor.
Adaptation of the Scanner's Pulse Sequence for Gradient-based Tracking
Reference is now made to
To achieve high quality tracking, the gradient-based tracking system as disclosed in U.S. Pat. No. 6,516,213 requires sufficient and independent activation of each of the three gradient fields of a standard MRI scanner. Each specific mode of operation of the scanner has a pulse sequence design (PSD) to achieve optimal images with specific requirements such as size, resolution, contrast, level of sensitivity to flow, etc. In the present invention, the method includes modification of the PSD by adding gradient activations for tracking. The added gradient activations may be used by themselves or in combination with the standard gradient activations. The advantages of this method are increased update rate of tracking and higher accuracy of tracking. Instead of only receiving tracking information during a regular scan, which may require tens of milliseconds or more, in the present method tracking information can be obtained every few milliseconds or even more frequently, by inserting additional specific gradient activations for tracking. This can be done for any standard PSD and may be accomplished in various ways.
In one embodiment, consecutive gradient activations of the three gradients that have zero net effect on the sample are added. This can be achieved by bi-modal gradient activations, where the negative activations null the effect of the earlier positive gradient activations (
In another embodiment, a single activation of one gradient is added for each phase encode step, and one can then toggle between the three gradients. Thus, within three phase encode steps, all three gradients are activated for tracking.
In another embodiment, only one or two gradient activations are added. In some scan modes two gradients are maintained with constant activation, while one gradient that serves as the phase encode gradient has variable amplitude in time. Thus, gradient activations for tracking can be added only to the variable phase encode gradient.
In yet another embodiment, a random phase encode is used for scanning. In this case the amplitude of the phase encode gradient is modified randomly, so there is no section with only low amplitudes.
Tracking with the Added Gradient Activations
When tracking is based on the additional gradient activations that are dedicated for tracking, as in the present invention, the scanner can be programmed to send a trigger signal to the tracking system. This trigger signal activation can be programmed as part of the pulse sequence to be activated during the time of gradient activations for tracking (
Independent activation of all three gradients may enable accurate determination of the location and orientation by the gradient-based tracking system. The typical time needed for single gradient activation on a standard high-field scanner (e.g. 1.5 T scanner) can be less than 0.5 msec, and a full packet duration can thus be less than 1-2 msec. Sufficient update rate for the tracking system depends on the application. Surgical intervention can be guided with an update rate of a few hertz (e.g. 4 Hz on the Signa-SP surgical scanner, GE Healthcare); motion artifact elimination requires a rate of at least 25 Hz or more, to enable tracking of physiological tremor with a frequency contents of up to 12 Hz (Deuschl G, Elble R J. The pathophysiology of essential tremor. Neurology 2000; 54:S14-20; Elble R J. Origins of tremor. Lancet 2000; 355:1113-4); catheterization may require the fastest tracking rate to compensate for cardiac motion, with frequency content that may reach 50 Hz (Schmidt E J, Dumoulin C, Hayase M, Brady T J. Coronary Artery Motion Tracking in Swine Models with MR Tracking American Heart Association Meeting, Orlando, Fla., Nov. 9-12, 2003). For these different applications, the time occupied by the tracking gradient activations will be less than 1% for surgical applications (5 activations of 2 msec, totaling 10 msec per second), about 5% for motion artifact elimination (25 activations of 2 msec per second), and about 10% of the scan time for cardiac applications (50 activations of 1-2 msec per second).
Tracking with Repetitive Gradient Activations for Tracking
Since the gradient activations for tracking can be accurately repeated at different times during the scan, a simpler hardware setup can be used by acquiring only the trigger signal and not the gradient command signals, as is typically used by the tracking system. Once the relation between the time of the trigger and the time of each gradient activation for tracking is determined and maintained throughout the scan, and the pattern of gradient activation for tracking is known and does not change with time, one can avoid the acquisition of the gradient signals. Thus, once a trigger signal is identified, the time of gradient activations can be determined by using the fixed delay between the trigger and the gradient activation (
Reference is now made to
Rather than using acquired gradient signals for tracking, as described above with reference to
The Effect of Voltages Induced by the Bo Field when the Sensor Moves or Rotates
Reference is now made to
Equation 4 provides the general description for the induced voltages in the sensor coils, but the description above assumes no effect of the Bo field. This assumption is correct as long as the sensor does not move, or when the movements are relatively slow. Since the typical rise time of gradients in modern MRI system is less than 1 millisecond (
When gradient activations for tracking are used, the effect of Bo on the signals can be eliminated in a more robust way. Since the Bo is constant, it induces electric potential in the sensor coils only when there is rotation of the coils which changes the magnetic flux through the coils. Unlike equation 3 above, the time varying variable now is the direction of the coils which is given by a time variable unit vector n(t):
Θ= G·n (t)A (8)
The induced electrical potential is continuous, it is not affected by the gradient activations for tracking, and it appears in the coil signals as a wandering baseline (
Embodiments of the Sensor
One embodiment of the sensor includes a configuration of three coils. Another embodiment includes a configuration of two coils, using the same approach as presented above, and using the 6 potentials induced by the three MRI gradients in the two coils to calculate the 6 unknowns (3 locations and 3 rotation angles). However, for optimal performance more data should be used to reduce the effects of noise and to improve the accuracy of the tracking.
A potential configuration with three orthogonal coils is presented in
A more complex configuration is presented in
A configuration in accordance with another embodiment is presented in
In a variation of the cylindrical hollow configuration the two pairs of "saddle" coils may be replaced by two planar coils, which may be positioned inside or outside the lumen of the cylindrical coil. Although this configuration partially blocks the catheter lumen, it is simpler to manufacture and may be useful with applications which do not require free lumen.
The sensors can be assembled from individual coils, for example by gluing 6 small flat coils on the 6 surfaces of a cube. On a catheter, one pair of coils has a cylindrical shape and can be directly wired over the shaft of the catheter, while the two other pairs have a saddle shape, and can be glued around the cylindrical coils. Another potential approach for the construction of the multi-coil sensor is by using flexible printed electrical circuits, which include all the coils and are folded to achieve the 3-dimensional shape.
Clinical Applications
The determined location and orientation of the sensor can be transferred to the MRI scanner in real-time and used for various tasks, for example for real-time control of the scanning plane, to display the location and orientation of the object or the device with the tracking sensor on the MR image, to correct motion artifacts. Potential clinical applications of the invention can be divided into applications for diagnostic MR imaging and for interventional MRI.
Diagnostic MRI: A major problem with MR imaging is motion artifacts due to patient movement. With high-resolution scanning, which may require image acquisition during many seconds and even minutes, patient movement and breathing may induce motion artifacts and blurred images. MR scanning is specifically sensitive to movements during phase contrast angiography, diffusion imaging, and functional MRI with echo-planar imaging (EPI). Using the present invention for real-time determination of the location and orientation of the scanned object can reduce the effect of motion on the MR scans by real-time control and correction of the scanning plane, in order to compensate for the movement, or by post-acquisition image processing. By using specific activation patterns designated for tracking, the real-time determination of the location and orientation can be made more frequently.
Interventional MRI: The sensor can be used with various devices, like miniature tools for minimally invasive surgery, catheters inside blood vessels, rigid and flexible endoscopes, biopsy and aspiration needles. It can be used to measure the location of the device with respect to the MRI coordinate system and to enable the MR scanner to present the device location on the MR images, as visual feedback to the operator, or to calculate and display the line of current orientation to assist the operator to steer the device into a specific target. Another potential application is to slave the MRI plane of imaging to the tracking sensor, for example to apply high resolution imaging on a small volume around the site of a catheter, for better imaging of the region of interest to improve diagnostic performance or to control the effect of an intervention (e.g radio-frequency, cryo, or chemical ablation and laser photocoagulation can be monitored by temperature-sensitive MR imaging). Another potential application is to use the information of the location and orientation of the device in order to enable display of the MRI images in reference to the device local coordinate system, as if the operator is looking through the device and in the direction of the tip, similar to the use of optical endoscopes. One more application is to use the location tracking in order to mark location of previous interventions on the MRI image.
An application with great clinical importance, where using MRI guidance is of specific advantage, is percutaneous myocardial revascularization (PMR). PMR is typically performed during cardiac catheterization. A laser transmitting catheter is inserted through the femoral artery up through the aorta into the left ventricle of the heart. Based on prior perfusion studies (e.g. Thallium scan) or indirect information on viability of the myocardium (e.g. by measurement of local wall motion), the cardiologist applies laser energy to drill miniature channels in the inner portion of the heart muscle, which stimulates angiogenesis and new blood vessel growth. PMR potentially provides a less invasive solution (compared to bypass surgery) for ischemic heart disease patients which cannot be adequately managed by angioplasty or stent placement. It may also be used in conjunction with angioplasty or stents to treat areas of the heart not completely revascularized by a balloon or stent placement. Currently, PMR is exclusively done with X-ray guidance. The main advantage of MRI is the excellent performance of MRI in the assessment of myocardial blood perfusion, through the use of contrast agents. Thus rather than using indirect information on the location of poorly perfused regions, a diagnostic session of myocardial perfusion in the MRI scanner can be followed by immediate intervention, using the existing perfusion images and real-time tracking of the laser catheter with the disclosed tracking methodology. An additional advantage, unique to MRI, is the potential to control the intervention by high-resolution, real-time imaging of the myocardium during the application of the laser treatment. Furthermore, since PMR is typically performed on multiple locations, and good coverage of the treated myocardium should be achieved, marking the location of the treated locations on the perfusion image, using the location data of the tracking system, may provide optimal coverage of the diseased region.
Anatomically, the tracking sensor can be used for various diagnostic and interventional procedures inside the brain (internally through blood vessels or through burr holes in the skull), the cardiovascular system (heart chambers, coronary arteries, blood vessels), the gastro-intestinal tract (stomach, duodenum, biliary tract, gall bladder, intestine, colon) and the liver, the urinary system (bladder, ureters, kidneys), the pulmonary system (the bronchial tree or blood vessels), the skeletal system (joints), the reproductive tract, and others.
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Right now I'm in the process of both upgrading and downsizing my wardrobe. Which means a big purge of stuff that I don't like or haven't worn in ages, and hopefully its slow replacement with pieces I love. So far I've added these boots and this skirt to my closet, but it's been so long since I've really shopped anywhere other than thrift stores that I'm having trouble actually finding clothes I like. I'm not sure if it's because my taste doesn't match what's available right now, or if I just haven't gotten in the groove yet. This weekend I'm going to Los Angelos, though, and I'm hoping to do some shopping there. Is there anyplace I should be sure to hit up?
Top: Thrifted
Skirt: Rag & Bone, consignment (similar)
Boots: Acne
Hat: Thrifted, upgraded


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SAS Channel Program Takes on WorldBy Jessica Davis | Print
Desktop-as-a-Service Designed for Any Cloud ? Nutanix Frame
The BI and analytics software vendor is expanding its year-old channel program globally.Business and analytic software maker SAS is expanding its year-old channel program geographically and is also enhancing the offerings partners in the Americas can offer to customers.
SAS said it will expand its channel program globally. Previously focused just on partners in the Americas, the new program will first expand to Canada, the U.K., France, Germany, Switzerland, Sweden, Italy, South Africa, Russia, Japan, China, Australia and India.
SAS has signed more than 60 "top-level" industry VARs in the Americas, the company said. Since its launch just a year ago, the Cary, N.C., company's revenue from channel sales has grown to about 4 percent of revenues, according to Miles Mahoney, vice president of global alliances and channels.
With an eye to capturing more business from the SMB market, which the company defines as those with less than $500 million in revenues per year, the company has introduced nine new product bundles for channel partners looking to gain SMB business.
"We've really put some thought into what offerings are needed to cover what this market segment has been asking for," he said. The bundles are specially priced for the SMB segment and are based on the SAS Enterprise Intelligence platform, the company said. They include SAS data integration, business intelligence and analytics software.
Last year's channel program launch focused on partners reaching customer organizations of $1 billion or less in revenues. The new bundles take the SAS target deeper into the SMB space.
"The new pricing bundles offer midmarket organizations the full breadth of SAS analytics capabilities to a wider market," said David Septoff, CEO of Zencos Consulting in Durham, N.C., in a statement. "The SAS reseller program is already adding value to our marketing and sales strategy and management."
SAS charges customers a first-year fee of 35 percent, and year-over-year customers pay a renewal, said Mahoney. "We are going to give 25 percent of that annual renewal to channel partners," he said.
The new global channel program is a mirror of the Americas program with a localized flavor, said Mahoney. SAS took 60 to 75 percent of the program elements that worked in the Americas and duplicated them to create the global program.
What Partners Need to Know About HP, ...In the channel, HP, Inc. is a storied vendor that has relationships...
Watch Now


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The Bucks New University is offering available 100 individual scholarships of up to £3,000 for new students starting year 1 of their course from September 2016 onwards.
The BSP is a scholarship designed to provide a direct benefit to individual students from England and the European Union who come from low-income backgrounds and is intended to support the progression of students from groups currently under-represented in higher education. All funds are provided by the University.
Buckinghamshire New University is a public university with campuses in High Wycombe, Buckinghamshire and Uxbridge, West London. The Vice-Chancellor of the University is Rebecca Bunting.
Scholarship can be taken in the UK
College Admission Requirement
Entrance Requirement: Entry requirements vary for each course; the student can find entry criteria by searching for their course of interest in their r course finder. As a guide, the academic entry requirement for undergraduate courses includes the achievement of the equivalent of a minimum of 2 UK A-level qualifications as confirmed with comparison information on UK NARIC.
Test Requirement: No
English Language Requirement: Applicants require IELTS overall score 6.0.
Students from England and the European Union can apply for this scholarship programme.
What is the study subject?:
Scholarship is awarded to study the subjects offered by the university.
The necessary course level:
Scholarship is available for pursuing undergraduate programme.
How much is the scholarship award?:
Each scholarship will equate to a financial support package of up to £3,000 which is paid as follows:
Criteria to be eligible:
Students are eligible to apply for a scholarship if their declared residual household income is £25,000 per annum or less, if they are entering university for the first time, have firmly accepted an offer of a place at Bucks, have applied for Student Finance and will be paying tuition fees in excess of £6,000 per annum (pro-rata for part-time students).
How to apply this scholarship?:
Once the student has firmly accepted their place with University via UCAS Track, they will need to complete a BSP application form and return it via email.
Find the application form in: View form


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November 21, 2017
Thanksgiving Break – Thanksgiving break will begin Wednesday, November 22nd at noon. Students will be dismissed early that day and have no school through Monday, November 27th. School will be back in session on Tuesday, November 28th.
Out to Lunch – Students who purchased the out to lunch certificate with merits will be going on Tuesday, November 28th. They will not need a lunch that day.
School Pictures – We are doing school pictures on Wednesday, November 29th. We are asking all students to dress in their Sunday best for their photos. Picture packages will be available for sale, and the information on those will be sent home with this newsletter.
Basketball – Practices will start December 1st and be Tuesdays and Fridays from 3pm-5pm. There is a tournament next month in Seneca. Pastor Schwender will be giving more details in practices when they start.
Christmas – We will be having a Christmas party on the afternoon of December 21st. This is the last day of the semester and will begin Christmas Break. Christmas break will extend through January 3rd with school starting again on January 4th. At the party, we want to encourage all of our students to bring a gift that will be exchanged. We will have the boys in each separate learning center exchange with each other, and the girls will do the same. We ask that gifts be valued around $10 so they are similar when exchanged.
Box Tops and Chick-Fil-A – Please continue to collect and send in box tops and Chick-Fil-A receipts throughout the year.
Please let me know if you have any questions.
Pastor Lear


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Status: Special Order
Brand: Fashion Bed Group
*Goes with any style, from contemporary to traditional.
*Solid wood frame is upholstered in a beautiful Grande Pearl 100% polyester fabric.
*A row of nail heads line the top of the headboard, delicately outlining the gorgeous fabric.
*Walnut posts are height-adjustable to 45", 49", and 53" to fit your existing bed.
*Available as a headboard only.
*Full/Queen, King/Cal King.


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Eva Nogales's lab is dedicated to gaining mechanistic insight into two important areas of eukaryotic biology: central dogma machinery in the control of gene expression, and cytoskeleton interactions and dynamics in cell division. The unifying principle in her work is the study of macromolecular assemblies as whole units of molecular function by direct visualization of their architecture, functional states, and regulatory interactions. To gain a molecular understanding of their systems of interest, her lab uses state-of-the-art cryo-electron microscopy (cryo-EM) and image analysis, as well as biochemical and biophysical assays.
Cytoskeleton and Cell Division
Microtubule structure, dynamics, and interactions. A long-standing interest of my lab is the mechanistic understanding of the process of microtubule dynamic instability by which microtubules switch between growth and shrinking phases powered by GTP hydrolysis in tubulin. We have characterized tubulin polymers proposed to mimic the structural intermediates in the processes of microtubule assembly and disassembly, and we are particularly interested in the role of the nucleotide state in microtubule structure and stability. Recently we developed an image-processing scheme that takes advantage of the pseudohelical symmetry of the microtubule to produce record resolution structures (better than 5 Å) for microtubules in different nucleotide states or bound to Taxol. This work has been a long-term goal of the cytoskeleton field and represents a quantum leap in our understanding of what makes microtubules so special.
Nogales Research Abstract Slideshow
Figure 1: Overall architecture of the Ndc80 kinetochore complex (left). Cryo-EM structure of the microtubule-binding head of the Ndc80 complex bound to a microtubule with a tubulin monomer repeat (right).
Adapted from Alushin, G. et al. 2010. Nature 467:805–810.
Figure 2: 3D model of the budding yeast kinetochore bound to a depolymerizing microtubule. The cryo-EM structure of the Dam1 complex is shown in blue, the microtubule is green, the nucleosome is dark purple, inner kinetochore complexes are shown in light purple, the Mtw1 complex is pink, the C-terminus of Spc105 is orange, Ndc80p is light blue, Nuf2p is gold, Spc24 is green, Spc25 is red, Ndc80p's unstructured N-terminus is magenta and Ndc80's coiled-coil is grey.
Adapted from Alushin, G. and Nogales, E. 2011. Current Opininon in Structural Biology 21:661-669.
Figure 3: EM images and schematic diagrams of the yeast septin rod, and paired filament assemblies. The yeast hetero-octameric septin complex is a linear rod with the subunits arrayed in the order and with the interfaces indicated. The rod is nonpolar because it has a two-fold axis of rotational symmetry running left to right between and orthogonal to the central pair of Cdc10 septins. The C-terminal extensions of the two Cdc3 and Cdc12 pairs project from the same face of the rod and presumably associate to form parallel coiled coils that are essential for rod stability. Filaments form via end-on-end assembly of the rods mediated by Cdc11-Cdc11 interaction through an N-C interface.
Adapted from Bertin, A. et al. 2008. Proceedings of the National Academy of Sciences USA 105:8274–8279.
Figure 4: Projection image of a section of the Saccharomyces cerevisiae bud neck, showing septin filaments abut the cell membrane (left). Segmented tomogram showing septin filaments running both parallel and perpendicular to the mother-bud axis (right).
Adapted from Bertin, A. et al. 2012. Molecular Biology of the Cell 23:423–432.
Figure 5: Human TFIID structure: alternative conformations and binding of DNA.
Adapted from Cianfrocco, M.A. et al. 2013. Cell 152:120–131.
Figure 6: Human transcription preinitiation complex (PIC): organization of the human transcription PIC based on single-particle cryo-EM analysis.
Adapted from He, Y. et al. 2013. Nature 495:481–486.
Figure 7: Activation of Cas9: schematic of the lobe rearrangement triggered by guide RNA binding to Cas9 and by target DNA binding.
Adapted from Jinek, M. et al. 2014. Science 343.
Figure 8: Subnanometer cryo-EM structure of Cascade, showing six CasC subunits in alternating gray and blue, two CasB subunits in yellow, CasA in purple, CascD in orange, CasE in magenta, and crRNA in green.
Adapted from Wiedenheft, B. et al. 2011. Nature 477:486-489.
Figure 9: Cryo-EM structure of the yeast proteasome. The different subunits in the 19S regulatory particle are color-coded.
Adapted from Lander, G.C. et al. 2012. Nature 482:186–191.
Figure 10: 3.5 Å Microtubule Structure - lateral contacts between protofilaments. Details on the lateral contacts between alpha-tubulin subunits in the MT.
Credit: Rui Zhang and Eva Nogales
Microtubule dynamic instability is essential during mitosis, and a number of cellular factors, including structural kinetochore components, interact with the dynamic ends of microtubules and are able to modify the behavior of these polymers. Most importantly, outer kinetochore complexes use unique structures at microtubule ends to harvest the energy of the nucleotide-coupled assembly and disassembly processes to carry out cellular work. In collaboration with David Drubin and Georjana Barnes (University of California, Berkeley), we showed that the 10-subunit Dam1 yeast kinetochore complex assembles around microtubules into rings that move processively with microtubule ends, thus coupling microtubule depolymerization to directional movement. Cryo-EM studies of Dam1 rings around microtubules have allowed us to describe the structure and organization of the Dam1 ring and have shed light on the nature of Dam1 oligomerization and on the interaction of the Dam1 complex with the microtubule surface.
The Ndc80 complex is the microtubule-interacting component of the highly conserved KMN kinetochore network. Our lab has produced the subnanometer structure of Ndc80 bound to the microtubule, showing how the complex binds with a monomeric tubulin repeat at the intra- and interdimer interfaces. This binding is sensitive to tubulin conformation, has a stabilizing effect on microtubules, and is coupled to a self-interaction of the Ndc80 complexes along protofilaments that readily explains the cooperativity of the process. More recently we have dissected the roles of the essential Ndc80 protein tail in microtubule binding and self-association and how such roles are regulated by the checkpoint kinase Aurora B. We have visualized, for the first time, the C-terminal tail of tubulin as it interacts with the Ndc80 complex in an adjacent protofilament, a novel mechanism of microtubule stabilization. In collaborations with Stefan Westermann (Research Institute of Molecular Pathology, Vienna, Austria) and Andrea Musacchio (Max Planck Institute of Molecular Physiology, Dortmund, Germany) we have also characterized the organization of the whole KMN kinetochore complex. Our goal is to gather enough information on these systems to generate a comprehensive model of the robust and regulated interaction of chromosomes and spindle microtubules during mitosis.
Septin assembly and membrane interactions. Septins are highly conserved GTPases that form filaments in vivo and in vitro and are essential in a variety of membrane-remodeling processes, most notably cytokinesis. We are particularly interested in the combinatorial character of septin-septin interactions and the interplay between membrane interaction and septin self-assembly. In collaboration with Jeremy Thorner (UC Berkeley), we have shown how the four essential mitotic yeast septins (Cdc3, Cdc10, Cdc11, and Cdc12) and the nonessential Shs1 come together in functional complexes and how they interact within filaments. We have described a marked enhancement in assembly that is totally dependent on and specific to PIP2 (phosphatidylinositol-4,5-bisphosphate), a signaling lipid known to be localized at the bud neck and to be essential to the cytokinetic process. Our characterization of the bud neck ultrastructure has shown the presence of coexisting septin filaments running both parallel and perpendicular to the bud axis. The relationship between in vivo phenotypes and septin assembly, organization, and interaction with different proteins is a major interest of our lab.
Gene Regulation
Transcription regulation. The accurate initiation of transcription requires the assembly of a preinitiation complex (PIC) that includes the transcription factors TFIID, TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II (RNAPII). Regulation is achieved by gene-specific activators and repressors, cofactor complexes that mediate the interaction of the general machinery with sequence-specific activators, and protein complexes involved in the modification or remodeling of chromatin. Our lab is interested in characterizing the structure of these different components and how they interact to regulate transcription. A main effort has been to define the structure of the human TFIID. Binding of this general factor to the core promoter is the first step in the assembly of the whole transcriptional machinery.
In collaboration with Robert Tjian (HHMI, UC Berkeley), we obtained the first three-dimensional model of TFIID and showed the existence of significant flexibility within the complex, which we proposed could play a distinct role in directing the formation of an active PIC. We have also characterized a cell-type-specific TFIID complex containing TAF4b and studied the interaction of TFIID with differential activators. In a recent collaboration with James Kadonaga (UC San Diego), we have shown that TFIID coexists in two predominant states, differing dramatically in the location of lobe A (which contains both TBP and TFIIA) with respect to a more stable BC core. A novel conformation of TFIID, the rearranged state, interacts with promoter DNA in a TFIIA-dependent manner. Downstream promoter regions are bound by lobe C, while the upstream DNA sequence is bound within lobe A. We propose that the dynamic conformational landscape of TFIID may have regulatory consequences by providing specific structural targets that can be recognized by transcriptional activators and repressors, and we are continuing to test this idea.
Recently, we have developed an in vitro reconstitution system to describe the stepwise assembly of the human PIC. This study allowed us to describe how TFIIF stabilizes the core promoter DNA along the surface of RNAPII, and how the addition of TFIIE results in the topological trapping of the DNA on the RNAPII cleft. TFIIE positions TFIIH so that the active ATPase in transcription initiation, XPB, is downstream of the transcription start site. We used an artificial DNA template that served as a mimic of that generated naturally by the helicase action of TFIIH. The apparent movement of downstream DNA in this structure, together with the positioning of XPB, suggests how XPB would act as a DNA translocase whose activity would push against the stably bound upstream DNA at the TATA box to induce negative supercoiling at the transcription start site. Our lab has also provided the first description of the repressive interaction of human RNAPII with noncoding RNAs (ncRNAs) (in collaboration with James Goodridge, UC Boulder), and by the RecQL5 helicase, which defines a new paradigm in eukaryotic transcriptional regulation.
Gene silencing. Polycomb repressive complex 2 (PRC2) is essential for gene silencing, establishing transcriptional repression of specific genes by trimethylating lysine 27 of histone H3. Despite its biological importance, little was known about PRC2 architecture and subunit organization. Our lab reconstituted a tetrameric human PRC2 complex (Ezh2/EED/Suz12/RbAp48) with its cofactor AEBP2 and obtained the only available structural description of the complex (20-Å resolution). We used a tagging strategy to position all functional domains within the complex that showed that the Ezh2's SET domain forms a core with the two activity-controlling elements, the WD40 domain of EED and the VEFS domain of Suz12. Our next goals include determining how PRC2 interacts with nucleosomes or with ncRNAs.
Posttranscriptional regulation. In addition to our core transcriptional studies, I am collaborating with a number of my colleagues on the structural characterization of essential complexes in key regulatory, posttranscriptional processes. These studies include the translation machinery in cap-dependent and cap-independent initiation, in collaboration with Jamie Cate (UC Berkeley) and Jennifer Doudna (HHMI, UC Berkeley); studies of RNAi complexes and the bacterial CRISPR system, both in collaboration with Jennifer Doudna; and our work on the yeast proteasome with Andreas Martin (UC Berkeley).
Portions of this work are also funded by the National Institute of General Medical Sciences.
As of March 3, 2016


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Way back in say, September, when I start to go a little crazy and just can't wait for Christmas, I started envisioning sugar plums and everything fabulous I would do to decorate our house for Christmas.
It involved something like
I found it on Pinterest. By the way you can check out my pinterest boards
Vastly exciting, I know. Anyway, so I purchased giant Christmas ornaments, mesh, springing glittered things, festive picks, you name it. I was a regular shopping Ninja, watching for the 50% off at Hobby Lobby to occur...able to get in and get out to check it out and then when it happened, out of nowhere I pounced :)
So as I was saying back in September I decided on this and in October I bought all of the supplies. I was ready.
But here the ornaments sit today...on a bench in our foyer. Ready to be hung, already. Hello?
And here is only ONE of the bags of mesh and supplies from Hobby Lobby, still just waiting to be dragged out of the closet and hung up. Still waiting...
And so here it is the 15th of December and I've made a decision. There are only 10 days left until Christmas, folks. And with gifts to wrap, a guest room to ready, menus to plan, and children to love on by the light of the tree, I am saying enough already.
Because whether I've realized it or not the stress of a to-do list still needing to be to-done is hanging over my head like an albatross. So no more. Tonight I am returning all of the materials. I could keep them, yes, but I am really trying to minimize the amount of crap I have laying around for projects I may one day get around to. And so, quite frankly, I just don't need it hanging around or over my head for another year.
I encourage you to do the same. Maybe you don't have all of the Christmas decorations up yet. Maybe you have this and that and that and this to do before the big day.
Let. it. go.
Go snuggle with your children.
Pour yourself a glass of milk chocolate coconut milk (available at Whole Foods and AMAZING!!) and address some cards to loved ones.
But cut yourself some slack, remember the true meaning of Christmas and try to simply enjoy the moments while you can.
You have my permission to blow it off. Until next year :)