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When I (Steph) moved into my house 3 years ago I quickly realized I needed to figure out how to take care of my lawn. I had three options:
Mom found a reel mower (manual push mower) at a yard sale and brought it to me to try out. I was not sure this "old fashion" mower would do the job, but I was really surprised at how well it worked. I also invested in a manual edger. Look below for some pictures of these tools in action!
I have been using these tools for 3 years and they have worked out well. We are interested in trying some battery powered lawn tools in the future to compare them.
If you have a small yard like me, these tools might be a great solution for you. They are eco-friendly and very quiet which is a plus!
Please note: We are not sponsored by any of the companies/products that we used. These products were picked and bought by us.
FYI: We are not professionals, and we don't claim to be. This is what we found worked for our project. Yours may need a little different approach. Safety first!
FYI: Affiliate links are being used on this page. Purchasing via these links help support MDP!
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Steph & Vicki
Welcome to Mother Daughter Projects!
We're homeowners sharing our DIY adventures as we learn to maintain, improve, decorate, and
use tech in our homes.
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Oklahoma president David Boren said conference stability "has been our first goal" throughout discussions of realignment and that the Sooners "look forward to achieving that goal through continued membership in the Big 12" rather than relocating to another league.
Boren issued a statement in response to the Pac-12's announcement Tuesday night that the league would not expand beyond its existing 12 teams, a decision that effectively removed the option of a four-team move toward the West Coast by Oklahoma, Oklahoma State, Texas and Texas Tech. Boren said OU officials "were not surprised" by the Pac-12's decision and had "decided not to apply for membership this year" before the announcement was made by Pac-12 commissioner Larry Scott.
In Scott's announcement, he said it was in the Pac-12's "best interests to remain a 12-team conference. While we have great respect for all of the institutions that have contacted us, and certain expansion proposals were financially attractive, we have a strong conference structure and culture of equality that we are committed to preserve."
The "culture of equality" refers to Pac-12 rules mandating equal TV revenue distributions among league members, a practice that is not the case in the Big 12.
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After a Monday meeting of Oklahoma regents, where Boren was empowered to take action on behalf of the school in regard to realignment, Boren identified the Pac-12 and the Big 12 as the Sooners' options. Texas officials have maintained throughout the realignment process that their primary goal was to preserve the Big 12.
In his statement following the Pac-12's announcement, Boren said: "We were not surprised by the Pac 12's decision to not expand at this time. Even though we had decided not to apply for membership this year, we have developed a positive relationship with the leadership of the conference and we have kept them informed of the progress we've been making to gain agreement from the Big 12 for changes which will make the conference more stable in the future. Conference stability has been our first goal and we look forward to achieving that goal through continued membership in the Big 12 Conference."
After Monday's regents meeting, Boren made it clear that the Sooners had issues with the status quo in the Big 12. He cited a lack of trust and leadership within the league, among other concerns.
In a statement released Wednesday morning, athletic director Joe Castiglione said: "I'm in full support of President Boren's announced decision for OU to remain in the Big 12. The reforms which he has been discussing with the presidents in the conference are very important to the future stability and strength of the Big 12 Conference." Football coach Bob Stoops said in a written statement that we was pleased that OU is staying in the Big 12.
In an article posted Tuesday on the website of The Oklahoman, the newspaper reported that the school would consider staying in a "reformed" Big 12 only if the league would increase constraints on the Longhorn Network _ Texas' 20-year, $300 million venture in partnership with ESPN _ and would remove commissioner Dan Beebe from his post. The report cited an unnamed, high-ranking Big 12 school source as the individual detailing the Sooners' conditions for remaining in the league.
Texas men's athletic director DeLoss Dodds and Beebe declined comment in regard to The Oklahoman report. It remains unclear this morning what, if anything, will change internally within the Big 12 as the league moves forward.
The Pac-12 decision means Texas A&M is expected to be the only school to leave the Big 12 after this year. The Aggies have been invited to join the Southeastern Conference for the 2012 football season, contingent upon a waiver of legal claims in regard to the move by Baylor and other Big 12 schools that have yet to agree to the waiver.

subset: commoncrawl


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Common Tasks
How to Provide Remote Support
The ability to connect to a users workstation from a distance and quickly solve the problem at hand has dramatically increased IT's coverage area. You no longer need a corporate IT budget and department behind you to offer remote assistance capabilities. Here's a few ways to get started...
Microsoft Remote Assistance (Free included with XP on)
MSRA offers a few options for offering or requesting help.Read full article...
Change Product Key to activate Windows 8 or Server 2012
Microsoft Activation has caused many to become perplexed on changing product keys for Server 2012 and Windows 8 operating systems. I ran into this issue while trying to upgrade my new Asus Taichi to Windows 8 Pro from the bundled home version with a MAK key. The following steps should be taken to change the product key in order to activate the upgrade.
To change the product key without first activating Windows, use one of the following methods:Read full article...
Add Users to local admin and remote desktop group using GPO
This tutorial will show you how to use the GPO Restricted Groups to add IT support technicians into the local administrator group of all PC's the policy is applied to. Using Group Policy to add users to the local admin group is a safer and better practice than providing domain admin privileges to technicians that do not have the need or experience to be working beyond the workstation level.
1.Define Security Group
First you need to define a security group in AD users and computers. In this example I am creating a security group called IT_TechRead full article...
Disable Internet Explorer Toolbars with GPO
Using GPO to disable Internet Explorer toolbars is an easy way to keep systems from running endless toolbars added usually without a user's knowledge. The method below will not prevent them from installing but will prevent them from running. This saves system resources, network bandwidth, and certainly screen space if things got out of control.
Start Group Policy Management
in windows XP; Start > Programs > Adminstrative Tools > Group Policy Management.
in Windows 7; Start (jewel) > Administrative Tools > Group Policy Management
Find and Edit
If your organization has multiple containers, find the pertinent container and right click on the GPO linked to it and select Edit.Read full article...
Deploy Network Printers using GPO
- Open Print Management Console in Adminitrative Tools (Start -> All Programs -> Administrative Tools -> Print Management)
- On the left sidebar you will see Print Servers under Custom Filters, right click Print Servers -> add/remove servers then you can either manually enter your print server name or browse for it.
- Click Add to List to be able to manage this print server with the Print Management Console
Important Files and Folders when Migrating to a new Computer
Important files and folders to migrate when transfering a user to a new computer.Read full article...


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DIY Wall Clock using Xyron and DCWV
Wow where did 2015 go too!. Time sure flies when you are having fun. Speaking of time (excuse the pun), my project this week is a super easy DIY clock. All you need is a cake board, your favorite DCWV paper and of course the absolutely essential Xyron Adhesive.
Xyron Creatopia with Permanent Adhesive
(Use a Xyron Creative Station for Smaller 9" Clocks)
Liquid Gloss Accents
White Satin Ribbon
Clock Mechanism
12" Cake Board
How it's made
Thanks so much for stopping by today, make sure to follow Xyron on Facebook
I'm also on facebook and if you want to stop by and say hi !
See you again soon.


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Devotional Blog:
Christian 'Culture', Depravity and Salvation, 2/29/2012, Ephesians 2:8-9
I love meeting new people, hearing new points of view listening to life adventures, life realizations and commiserating on mutual experiences. So 2012 is a leap year and there was no entry in my devotional book for this day so this is my own devotional. Honestly I do have any number of pages in the book dog eared to write about as I am fantastically behind in my posting, but I find I enjoy writing more when I am writing about something that is currently bothering or inspiring me. Enter today's topic.
I had the pleasure of meeting someone with a very similar upbringing to myself and we bantered back and forth about being children having grown up in the church. Children who grew up in the church, most likely said their 'salvation' prayer at a young age, went through the 'Christian' motions growing up, sunday school, youth camp, retreats, revivals, door to door evangelism, whatnot. We 'shunned' the people we were supposed to shun or hate, we accepted the people that fit into the Christian box and we were encouraged that the greatest calling in life is that of ministry. Christian culture surrounded us, we memorized verses, held our hands up in deference to God during worship, allowed people to pray for us, we prayed for people, we knew all the ins and outs of the culture and we really didn't have an understanding of what true 'salvation' was…but of course we were saved…weren't we? Continue reading "Salvation and Sugar Damning…"


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I'm going to go on record and say Warcraft was the best movie of the summer no one bothered to see. According to the box office it was a major flop, which on one level I can kind of understand. Ever since Peter Jackson turned The Lord Of The Rings into three superb movies that's pretty much been the last word on fantasy flicks. And here comes Warcraft mining the same territory with a world full of elves, dwarves, magical fauna, orcs and magic-users. I know, I know, we've seen it all before, but Warcraft put it's own unique spin on the subject matter. I guess it still suffered from that been-there-done-that vibe. I'm not into video games, so I can't compare how the movie did in adapting it. All I've ever known of Warcraft was that it was a video game. That's it. Maybe the lovers of the game didn't think the movie held up, or maybe their demographic wasn't enough to make it a success even though they loved it? All I can do is reiterate that Warcraft, for me, was the best tent pole flick of summer 2016 with a story that was much more satisfying than any of the super hero flicks DC and Marvel put out this year. Yeah, I thought it was that good!
In this world there's a lethal kind of magic in existence called the Fel, and it kind of acts like a form of psychic vampirism, a concept that doesn't get enough love in horror films, if you ask me. Wielding the Fel you can suck the the very life from any living organism and if you so choose can take that life and instill it into something dead to make it live again, but this is essentially an evil form of magic and those eager to use it end up being corrupted.
One of the things I loved about this movie is that it's told from both sides. You follow characters on the orc side as well as the human side, and there is a faction of these invading orcs who think their Fel wielding leader, Gul'dan (Mo-capped bu Daniel Wu), is the real enemy and not the human dimension they're invading. This movie tells the first contact humans had with orcs and orcs are not of the same dimension humans live in. The orcs are invading our world because Gul'dan has essentially "drained" their current world of all life, but the thing is the orcs cannot just invade, they have to be invited in. Yes, there's a traitor on the human side who has helped in opening up a portal for them to come through. The second thing I loved about this flick is even though there's a lot of good and evil there's also a lot of gray, and some of our bad guys are really good guys and vice-versa, but our "traitor," even though he does fall into the "bad guy" category is the kind of bad guy who can understand.
Our other main characters come in the form of a young magic-user, Khadgar (Ben Schnetzer), who didn't like his training much and left, but he's supposed to be the new Guardian of this realm. The current one, Medivh (Ben Foster), has been in seclusion for a long, long time, and that's only because there hasn't been any Evil to contend with, until now!
Dominic Cooper plays King Llane Wrynn, Ruth Negge his Queen Taria Wrynn, Travis Fimmel plays Sir Anduin Lothar, who's in charge of the military forces, and he has a son, Callan (Burkely Duffield), who's in the military, too, and who meets an untimely end at the hands of orc Blackhand (Mo-capped by Clancy Brown). Don't worry Lothar gets revenge at the end. Caught between two worlds is half-orc, Garona (Paula Patton), who's more human looking. So that would mean at some point the orcs must have encountered a human at some point, though that is never gone into. The CGI is near perfect when it comes to the depiction of the orcs, the primary ones were all motion captured by aforementioned actors, Clancy Brown, Danial Wu, Robert Kazinsky, Toby Kebbell and Anna Galvin. I loved all of these characters and the actors who played them!
Back on September 27th Universal released Warcraft on separate five separate editions: A Blu-ray/DVD/Digital Copy, a solo DVD, a 4K UltraHD/Blu-ray/Digital Copy, a 3D Blu-ray/Blu-ray/Digital HD and a Warcraft Exclusive – Bonus 8 Collectible Character Cards (Blu-ray/DVD/Digital) edition.
Video/Audio/Subtitles: 1080p 2.40:1 high definition widescreen—7.1 English Dolby Atmos 7.1 English Dolby TrueHD 7.. 5.1 Spanish Dolby Digital, 5.1 French Dolby Digital—English SDH, French, Spanish
Extras included . . .
- Deleted/Extended Scenes (13:57 )
- Gag Reel (3:25)
- The World of Warcraft on Film: Origin Story (4:54); The World of Talent (5:35); The World of VFX (5:09); Outfitting a World (6:16); The World of Mo-Cap (6:50); The World of Stunts (5:06).
- The Fandom of Warcraft (6:36): A look at Blizzard's and Warcraft's die-hard fans.
- Warcraft: Bonds of Brotherhood Motion Comic (53:47)
- Warcraft: The Madame Tussauds Experience ( 7:32)
- ILM: Behind the Magic of Warcraft (2:59)
- Warcraft Teaser – 2013
I wish this movie had made money because it ends with the potential of more to come and some crucial events unfold that really need a second chapter.


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Golden Records was a record label founded in 1948 by publisher Simon and Schuster. Many different cartoon-related records were issued over the years, including records from the Disney and Warner Brothers studios. The Hanna-Barbera–related records began in the late 1950s. Records were issued on 78 rpm and 45 rpm singles, as well as full-length LPs.
Album releases
- LP-51 Quick Draw McGraw and Huckleberry Hound: TV's Favorite Cartoon Stars (1959)
- GLP-55 Howl Along with Huckleberry Hound and Yogi Bear (1960)
- LP-60 Huckleberry Hound for President (1960)
- LP-66 Songs of The Flintstones (1961)
- LP-70 Songs of Yogi Bear and His Pals (1961)
- LP-90 How to Be a Better than the Average Child Without Really Trying (1962)
- LP-98 The Jetsons (1962)
- LP-120 Magilla Gorilla (1963)
- LP-124 Hey There, It's Yogi Bear (1964)


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The Passover of the Jews was at hand, and Jesus went up to Jerusalem. In the temple he found those who were selling oxen and sheep and pigeons, and the money-changers sitting there. And making a whip of cords, he drove them all out of the temple, with the sheep and oxen. And he poured out the coins of the money-changers and overturned their tables. And he told those who sold the pigeons, "Take these things away; do not make my Father's house a house of trade." His disciples remembered that it was written, "Zeal for your house will consume me." (John 2:12-17)
How much Christ disapproves all irreverent behaviour in the house of God! We are told that he drove out of the temple those whom he found selling oxen and sheep and doves within its walls, that he poured out the changers' money and overthrew their tables and that he said to them that sold doves, "Take these things hence; make not my Father's house an house of merchandise." On no occasion in our Lord's earthly ministry do we find him acting so energetically and exhibiting such righteous indignation as on the occasion now before us. Nothing seems to have called from him such a marked display of holy wrath as the gross irreverence which the priests permitted in the temple, notwithstanding all their boasted zeal for God's law.
The passage is one that ought to raise deep searchings of heart in many quarters. Are there none who profess and call themselves Christians, behaving every Sunday just as badly as these Jews? Are there none who secretly bring into the house of God their money, their lands, their houses, their cattle and a whole train of worldly affairs? Are there none who bring their bodies only into the place of worship and allow their hearts to wander into the ends of the earth? Are there none who are "almost in all evil, in the midst of the congregation?" (Prov. 5:14). Multitudes, it may be feared, could not give a satisfactory answer. Christian churches and chapels, no doubt, are very unlike the Jewish temple. They are not built after a divine pattern. They have no altars or holy places. Their furniture has no typical meaning. But they are places where God's Word is read and where Christ is specially present. The man who professes to worship in them should surely behave with reverence and respect. The man who brings his worldly matters with him when he professes to worship is doing that which is evidently most offensive to Christ.
- J.C. Ryle


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This Shift in consciousness is deep. It is fundamental and it is happening from the inside out. It is changing how you think, how you perceive and what you believe. It will alter your perception of the world around you and, more importantly, it will change your relationship to absolutely everything that you know. It is very exciting. It is very expansive.
One of the things that I am aware of is that in the beginning the Creator said, "I wish to know myself to the farthest edge of the farthest edge so that I can understand myself." In real terms the Creator was saying, "I wish to know myself in relationship to and with everything that is around me." This is about relationship, your relationship to everything around you and how you perceive it from within you.


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Evan Steinberg
Professional Experience
Prior to joining Rittenhouse Retail in March 2018, Evan worked as an Associate Salesperson at CBRE following their acquisition of FAMECO Real Estate, L.P. He has extensive experience as a tenant and landlord representative, working with various retailers, restaurants and commercial property owners in the Greater Philadelphia Area including PA, NJ and DE.
Since graduating from Tulane University in 2012 where he studied Marketing and Management Consulting, Evan has been responsible for identifying new landlord and tenant clients, establishing and maintaining client relationships, developing market roll-out and expansion strategies for retailers and restaurants, conducting market research and analysis, identifying and evaluating prospective sites and tenants, and Letter of Intent and Lease negotiations. In addition to working with smaller, local operators and franchisees, he has worked with larger national concepts such as Chipotle, AT&T, 7-Eleven, Five Below, Bloomin' Brands, Harbor Freight Tools, Domino's Pizza, Kilwins Chocolate, Miracle Ear, Title Boxing Club, iLoveKickboxing, USPS, Popeye's, Kumon, Robeks Smoothies, Goodyear and Core Power Yoga. Recently, Evan's primary focus has been on procuring clients seeking locations in downtown Philadelphia and multi-unit expansions into the surrounding area.
As a landlord representative, Evan has assisted property owners and developers in leasing retail space ranging from single storefronts to entire shopping centers, ground-up developments and redevelopments in urban and suburban markets. The ability to effectively market sites and communicate with clients has allowed Evan to find prospects and negotiate leases with many successful tenants for his listings and landlord clients. Evan strives to provide top of the line and unique service catered specifically to each client. As a result, he has developed and maintained relationships with a long and diverse list of retail and restaurant concepts and property owners. With his extensive knowledge of the real estate markets and retail trends in the Greater Philadelphia Area, along with the use of state of the art resources, Evan is able to get the best possible results for his clients.
Professional Accreditations/Affiliations
- Licensed Real Estate Salesperson in PA, NJ
- Tulane University, Bachelor of Science in Management Degree; Marketing and Management Consulting
License: PA RS323547


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Tuesday, August 25, 2009
In praise of death
And yet, I want to sing death's praises. Not sudden death, or violent death, or death before time. Not death that is plotted and planned and controlled. But death after a good life - yes, I will sing its praises.
I sat with my mother as she died, after years of suffering. We made jokes, and cried, and sang, and said goodbye; and then we were quiet as she laboured at the end. The room was filled with a sense of generosity, of hope and love. And freedom.
Because when a sick person dies, they are liberated from their suffering. They, and we, are utterly relieved that they will have to endure no more. No more doctors, no more pain, no more pills. And when a sick person dies, they leave a space. For years it is a great yawning void, a space that can barely be endured. And yet, whether we tiptoe round the edges and pretend it's not there, or leap into the void and feel drowned by grief, we grow into it. Our lives expand and we experience liberation.
My mother was a gifted woman, a workaholic, a trailblazer. She was large; her spirit took up most of the room. There wasn't much space for the rest of us. After she died, we crumbled down to our hard cores, then slowly, unexpectedly blossomed, as her shadow faded and we had access to the sun. So I will sing praises, to the end of suffering and to the start of something new.
And I will sing of corpses. I have seen a few. I will sing praises, because death is hidden here. Sick people are shuttled off to hospital, or hospice. To visit them and spend time with them, we have to drive to strange suburbs and sit in strange rooms and have strange conversations. Death becomes foreign. It happens to other people, somewhere else. Bodies are whisked away, and reappear, by arrangement, at the funeral home. So I will sing the praises of corpses, of the right to see a loved one in their final state, of the chance to say one last goodbye to a strangely still and waxy face, familiar and foreign in death.
I keep a few skulls on my piano: a wallaby, a kangaroo. I caress a bird's nest made of down, a hawk's feather. These objects of beauty are reminders of transience, a sign of the wonders to come.
And because I sing praises, I sing praises of life. Since it is finite, let us fall down into ourselves and discover who we are, so that when our time comes we can say that we have lived. Let us become those people today. Not tomorrow, not next week, not next year. Let us celebrate the life that is here, the life that is now, the life worth living. Let us turn up our stereos and dance round the kitchen. Make a cup of tea and watch the clouds scud across the sky. Plant some flowers in a surprising place. Write a letter to a loved one. Invite someone to dinner and open a bottle of wine. Start drawing, or writing, or singing like we mean it. Join a peace movement. Eat a juicy apple, and live extravagantly, generously, open-handedly today.
Because some day soon our bony friend will come knocking at the door. And when those hard knuckles go rat-a-tat-tat, I want to open it boldly, ready and able to welcome him in.
Posted by Alison Sampson at 12:55 PM
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Reviews for the Sacanagem
Review Guidelines – Take into consideration that reviews are subjective and do not always indicate which would be the best boat for you. We have done our best to include all reviews we receive for the boats on our sites (both good and bad) as long as we feel they will help our clients to make an informed decision. We reserve the right not to include reviews we feel are either unnecessarily offensive or unjust so feel free to look elsewhere for reviews we may not have published.
James Kilpatrick
I sailed on the 23rd of dec, due to weather we didnt make it to cartagena, had to go to sapzurro. We sailed with Federico on Sacanagem and it was first class, food was great and federico was a fantastic captain, very professional but also very funny. Would highly recommend him to future guests. – DECEMBER 2010
Amy Wilding
We sailed to cartegena with Fredrico on the 23rd December. The trip was awesome and we had a blast but unfortunately due to bad weather we were unable to complete the journey to cartegena and had to head to the nearest port. – DECEMBER 2010
Thomas and Carmen
As promised we send you a feedback about our boat trip one month ago with frederico from panama to cartagena. Fredercio was great and he cared a lot about our wellbeing. He made a lot of effort preparing good food and was very concerned about the safety. We were a bit disappointed because we excepted to go on a sailing trip. We can understand the reasons why he prefered the motor, but we would have prefered to know in advanced that we won't really sail. Maybe we would have choosen another way to get to colombia.(Editor's Note: Almost all the boats motor for most of the trip from Panama to Colombia due to the weather conditions and time involved in sailing) The other thing was that the snorkelling equipment was miserable. Nearly none of the masks were usable.(Captain reports this issue has been resolved) An idea: During the two days of sailing a lot of us were sea sick, and nearly nobody was able to do something. I really appriciated having a audio bool with me, otherwise it would have been very boring. Perhaps others like the idea…
More Reviews
– Charlotte`s travel blog


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I would also like to share with you the 2 layouts we made at convention and the Scroll Album. I will be offering the scroll album as a workshop.
This layout is Love Potion from Magic.
This layout is Levitate from Magic.
These are the dates I will be offering Clementine Workshops in September:
8th - Workshop on the Go
11th - Sticker Night
19th - Crop Night
28th - Scroll Album
Please email me about times and locations. If you cannot attend, I will do your cutting and stamping for you for a $5 fee. Then you will be able to put the kit together at your convenience.
Thanks for stopping by, and I hope to see you in September!


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Fudge fun for Pudding Lovers!
Fresh Food, Great Flavours and Fudgey Fun at the Fudge Pudding Lovers' Event
The perfect event for any fanatic foodie, pudding lover or sweet-toothed socialite, come and test your taste … Continue Reading »Read More
Call Us On: 01262 673 743
Welcome to the forum, feel free to introduce yourself inside the welcome thread and start new threads with any discussions you want to open. Advertisement of your services is allowed combined with healthy discussion.
If you want to request a quote for a service or offer your services at a discounted rate to other members please use the services thread
The perfect event for any fanatic foodie, pudding lover or sweet-toothed socialite, come and test your taste … Continue Reading »Read More
We would like to wish you all a very Happy New Year and a big warm welcome to our luxurious Bridlington Holiday Cottages. With t… Continue Reading »Read More
One of the UK's most distinctive comedians, Jimmy Carr, is kicking off his brand new tour, Funny Business, making its' way to Hull City Hall on Friday 21st November.
Please note: this pe… Continue Reading »Read More
Try A Taste of Bridlington with a Wintery Theatre Break
We are fast approaching wintery wooly hat weather, the evenings are drawing in fast and you may even see the twinkly lights being asse… Continue Reading »Read More
Enjoy a Halloween retreat this weekend and join Magic Mike with all his friends on Friday 31st October, beginning at 2:00pm, at Bridlington Spa.
Magic Mike has excited visitors returning year… Continue Reading »Read More
If you fancy a cheeky theatre break before the Christmas holidays get into full swing, why not pay a visit to Bridlington to see award-winning comedy musical Avenue Q?
From Tuesday 18th Nov… Continue Reading »Read More
This weekend, the hugely popular, BDO British Darts Open Championship returns to Bridlington Spa for the 40th staging of this highly ranked darts-focused tournament.
Firmly established as one… Continue Reading »Read More
A celebration of music, culture, arts, crafts, dancing, real ales and good food, the Hull Festival will bring you an assortment of the old and the new, from the 12th – 14th September, situated… Continue Reading »Read More
Magic Mike returns to Bridlington Spa Royal Hall, with a Summer Show packed full of magical comedy and fun for all the family!
Come along on Tuesday 5th August at The Spa Bridlington, to expe… Continue Reading »Read More
Following the previous success of the street Dance Experience at the Hull New Theatre, Skyline Studios are back for their third performance!
On Saturday 26th July at 7:30pm, one of the UK's p… Continue Reading »Read More
Showing 21 to 30, of 75 Blog Posts found


text: Im trialing both MapPoint and Tableau Software. Im trying to do something in MapPoint that I can do in Tableau, easily, but cannot accomplish in MapPoint. I want to import 500 revenue/sales records belonging to 12 distinct clients and 16 distinct zip codes. I want a map that will show sized circles for 16 locations using the client name as the label. I select the data field as revenue, select the show data by zip code, but does not allow me to select a combine data radio button, but only when Im selecting zip code not the other selctions. I would appreciate any help.


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This Thanksgiving so far - I've been to San Francisco, Sacramento, Denver & Nevada City - and am heading to West Palm Beach, FL tomorrow. Thankful for warm homes to welcome me in each town.
Just discovered lemlem, started by Ethiopian supermodel Liya Kebede to provide employment to skilled weavers in her country. Beautiful pieces for a beautiful cause.
Posted by Lynsay at 21:39 No comments:
Labels: shop, sustainability, textiles
Lap dog
Posted by Lynsay at 21:37 No comments:
Labels: henry
Selby in Japan
Adorable home, cutest family ever! From the Selby.
"I don't believe this gifted few concept, just in people doing things they are really interested in. They have a way of getting good at whatever it is." -Eames
Posted by Lynsay at 21:05 No comments:
Creatures of Comfort: LA Fall
A & D Museum: Eames
Charles and Ray Eames were masters of seeing 'the uncommon beauty in common things'. A new exhibition called Eames Words at the Architecture & Design Museum of LA displays their creative process, focusing more on their ideas than their design outcomes. I love the simple pleasures in life and can't wait to check out that which they considered beautiful.
Posted by Lynsay at 09:18 No comments:
Labels: architecture, art, design, museum
Polka Dots
A recent fave from The Sartorialist - wonderfully reminiscent of Katharine Hepburn's amazing polka dot jumper I blogged about a while ago.
Posted by Lynsay at 21:45 No comments:
Elke Kramer's jewelry collection Second Nature influenced by Ernst Haeckel's studies of plant cells.
Posted by Lynsay at 12:49 1 comment:
Looking forward to this:
Posted by Lynsay at 10:59 No comments:
Labels: architecture, art, design, film
Finn Juhl
A look at architect/furniture designer Finn Juhl's home in Ordrup, Denmark, which is now a museum. The hearth is my favorite, it totally makes the living room so inviting. And that painting of the woman in the yellow blouse, oh my. More photos here.
Posted by Lynsay at 10:55 No comments:
Labels: architecture, art, design, interiors, textiles
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Man Digs Himself Out– Escapes Stoning Death in Iran
An Afghani man managed to dig himself out of a ditch before he was stoned to death in Iran last week.
Iranian Rooz Online reported:
Three men were officially stoned in the city of Mashhad in Eastern Iran on Thursday, one of whom survived the ordeal.
An informed source in Mashhad told RoozOnline that three were stoned in the Behesht Zahra cemetery in Mashhad. Mahmud M.G of Afghan nationality succeeded in pulling himself out of the ditch where he had been buried for the stoning and escaped death. The other two dies of wounds inflicted by the stones. Under Islamic punishment, if a person can survive or escape the ordeal, s/he is considered kosher.
In Iran, unlike other Islamic countries, stoning is an official punishment for those who engage in sex with another married person. One of the wrongdoers by the name of Hushang K. had been sentenced for engaging in sex. No detailed reports about the crimes of the other two have been formally announced.
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John Hope Biography
Born on June 2, 1868, in Augusta, Georgia, John Hope graduated from Brown and, after teaching for some years, became the first African-American president of Morehouse College. He and colleague W.E.B. Du Bois shared a fervent belief in acquiring educational opportunities for black citizens. Having also directed college affiliations under Atlanta University, Hope died on February 20, 1936.
Background and Education
John Hope was born on June 2, 1868, in Augusta, Georgia, to Mary Frances "Fanny" Butts and James Hope. His mother was a free African-American woman of mixed ancestry while his father had immigrated to the states from Scotland, launching a successful cotton mill business. Though prohibited by race codes from marrying, the two lived together and had several children.
After his father's death, a pre-adolescent John went to work at a lawyer's office and then at a restaurant. With financial aid, he was able to attend Worcester Academy in Massachusetts, becoming a well-rounded student with interests in academics and athletics. He later earned his B.A. from Brown University in 1894, where he was chosen to be commencement speaker. Hope wed Lugenia Burns of Chicago in 1897, with the couple going on to have two children.
President of Morehouse
Upon graduation, Hope took on a teaching position at Roger Williams University in Nashville, Tennessee as a natural sciences professor, also serving as a football coach and taking additional classes at the University of Chicago. Then in 1898, he took on a position teaching classics at Atlanta Baptist College, doing so for some years before being appointed in 1906 the college's fourth president. He thus became the first African-American president of the institution, which would be renamed Morehouse College in 1913.
Under his leadership the school's enrollment improved greatly, and in later years Hope worked to establish an affiliated system under the rubric of Atlanta University, with Morehouse and Spelman colleges becoming partnered and the city's additional African-American educational centers to follow. Hope became university president in 1929.
Du Bois and Niagara Movement
He and famed writer/scholar W.E.B. Du Bois had developed a close friendship during Hope's initial days of teaching at Atlanta Baptist; the two shared major ideologies via their steadfast belief in educational attainment for African-American citizens and challenging the racist status quo. A published writer as well, Hope was one of the participants in the 1905 Niagara Movement meeting. Its trailblazing Declaration of Principles paved the way for the creation of the NAACP, on whose board Hope served.
He also travelled to Europe extensively during the second decade of the 1900s, visiting his Scottish homeland and working with African-American military personnel during WWI as part of the YMCA's National War Work Council, challenging prejudicial treatment.
Hope received several honorary degrees over the course of his lifetime, among other accolades. He died at 67 from pneumonia on February 20, 1936, in Atlanta.
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Flag Day
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Try Hollandse Nieuwe herring and enjoy the festivities on Flag Day or, as the Dutch call it, Vlaggetjesdag.
- Celebrate the first herring catch of the year with a wonderful festive programme in Scheveningen.
- Discover old Dutch crafts and see the beautiful fishing boats in the harbour of Scheveningen.
- Vlaggetjesdag is accessible free of charge.
Flag Day in Scheveningen is one of the biggest street festivals in The Hague. The flags in the spa town of Scheveningen flutter in the wind, music can be heard everywhere and even the youngest inhabitants wear traditional attire. The new herring catch of the year is celebrated on Flag Day. The Hague comes to the coast in droves to taste the first 'New Dutch' of the season.
New Dutch
The 'New Dutch' herring is caught between mid-May and the end of June. Earlier than this the herring is too lean; later than this it is too fatty. The first catch of the season is brought in to shore on Flag Day. The first cask is sold at auction and the profit is donated to charity.
Scheveningen festival programme
Although the herring catch is the occasion of the festival, there is more on the programme than sampling fish during this event. Festival-goers can participate in Old Dutch children's games, sing along with popular artists, tour boats, listen to bands and check out traditional handicrafts. And, of course, you can also watch all the happenings from one of the many terraces for which Scheveningen is known.
Flag Day is a free event that is held every year in June.
Spending the night in Scheveningen
Are you searching for a hip design hotel, a romantic bed & breakfast, or a pleasant holiday home for your family? Scheveningen has suitable accommodations for every taste and budget. Check out the comprehensive selection and book your perfect holiday here!
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Four seasons' worth of enjoying the beach, sports, great food and good company.Read more
Visit the most popular seaside resort and enjoy countless activities and sports.Read more
Discover the multifaceted offerings at Scheveningen Pier.Read more
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Surf the best waves in surfing hotspot Scheveningen. Rent a surfboard to catch some waves or sign up for a course.Read more
Various (inter)national sporting events are organised on the beach of Scheveningen between May and September.Read more


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- Research
- Open Access
Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells
- Quan Liu†1,
- Juan Liu†1,
- Kristina Irene Lisolette Roschmann2, 3, 4,
- Danielle van Egmond2,
- Korneliusz Golebski2,
- Wytske Johanna Fokkens2,
- Dehui Wang1Email author and
- Cornelis Maria van Drunen2
© Liu et al.; licensee BioMed Central Ltd. 2013
- Received: 22 July 2012
- Accepted: 3 April 2013
- Published: 11 April 2013
HDAC inhibitors have been proposed as anticancer agents. However, their roles in innate genes expression remain not well known. Cathelicidin LL-37 is one of the few human bactericidal peptides, but the regulation of histone acetylation on LL-37 expression in airway epithelium remains largely unknown. Therefore, we investigated the effects of two non-selective HDACi, trichostatin A (TSA) and sodium butyrate (SB), on the expression of the cathelicidin LL-37 in human airway epithelial cells. LL37 in human NCI-H292 airway epithelial cells and the primary cultures of normal nasal epithelial cells(PNEC) in response to HDAC inhibitors with or without poly (I:C) stimulation was assessed using real-time PCR and western blot. In parallel, IL-6 expression was evaluated by ELISA. Our results showed that HDAC inhibitors up-regulated LL-37 gene expression independent of poly (I:C) stimulation in PNEC as well as in NCI-H292 cells. HDAC inhibitors increased LL37 protein expression in NCI-H292 cells but not in PNEC. In addition, HDAC inhibitors significantly inhibited poly (I:C)-induced IL-6 production in both of the epithelial cells. In conclusion, HDAC inhibitors directly up-regulated LL-37 gene expression in human airway epithelial cells.
- Epigenetics
- Innate immunity
- Histone
- Deacetylation
- Toll-like receptor
- Cathelicidins
Recent studies suggest that epigenetics have an important role in regulating innate immunity and that the manifestation and severity of diseases may be influenced by epigenetic factors. Epigenetic modifications play an important role in the regulation of gene expression and a common mechanism in epigenetics is the control of the accessibility of the transcriptional machinery to promoter and enhancer elements in the genome. Histone modification through reversible acetylation is a crucial event in gene transcription regulation [1–3]. The net state of histone acetylation is regulated by the opposing actions of histone acetyltransferases(HATs) and histone deacetylases (HDACs). Small changes in the HAT/HDAC balance could affect transcription of many inflammatory genes, potentially having a profound effect on the initiation and duration of inflammatory responses [4, 5]. Yin et al[6] reported on bacteria-specific innate immune responses via epigenetic regulation in gingival epithelial cells.
The respiratory epithelium is an important interface with the environment and it is now well accepted that the epithelium is not only just a physical barrier, but plays an active role in innate and adaptive immunity [7]. Antibacterial peptides are an integral part of the epithelial defence barrier that provides immediate protection against infection. Cathelicidins are a family of antimicrobial peptides and LL-37, the only cathelicidin in humans, plays a critical role in the defension of epithelium against the microorganism and is produced by neutrophils, macrophages, and various epithelial cells as well [8].
Increasing evidence suggests that HDAC inhibitors down-regulated the expression of numerous host defense genes including pattern recognition receptors and cytokines. In this study, we wanted to explore the effect of HDAC inhibitors on the expression of LL-37 in airway epithelium in the context of the viral double-stranded RNA-mimic poly(I:C).
Materials and methods
Bronchial Epithelial Basal Medium (BEBM) with Bronchial Epithelial Growth Media(BEGM) SingleQuots were purchased from Lonza Walkersville, Inc. Poly(I:C), TSA, sodium butyrate, L-glutamine, pronase (type XIV protease) and 0.25% trypsin-0.02% EDTA were purchased from Sigma Chemical Co (St Louis, MO, USA). NCI-H292 human airway epithelial cells were purchased from American Type Culture Collection, Manassas, VA, USA and Shanghai Institutes for Biological Sciences (SIBS). Anti-Cathelicidin antibody was purchased from Abcam. Beta-actin antibody and HRP-linked antibody were purchased from Cell Signaling Technology. Fetal bovine serum, penicillin and streptomycin were purchased from HyClone (Logan, UT, USA). RPMI 1640 and trizol medium were purchased from Life Technologies, Inc., Gaitersburg, MD, USA. Cell Counting Kit 8 was purchased from Dojindo (Kumamoto, Japan). First strand cDNA synthesis kit was purchased from Fermentas GmbH(St Leon-Rot, Germany).
NCI-H292 human airway epithelial cell culture
NCI-H292 human airway epithelial cells were cultured as reported before [7]. Briefly, NCI-H292 cells were cultured in RPMI 1640 medium supplemented with 1.25 mM of L-glutamine, 100 U/mL of penicillin, 100 μg/mL of streptomycin and 10% (v/v) of fetal bovine serum in six-well plates. Cells were grown in fully humidified air containing 5% of CO2 at 37°C and were sub-cultured weekly.
Isolation and culture of human nasal epithelial cells
Primary nasal epithelial cells (PNEC) were isolated from normal middle turbinate that was obtained from patients who underwent endoscopic endonasal surgery in pituitary adenoma patients who had given their written informed consent in accordance with a study protocol approved by the Ethics Committee of Eye and ENT Hospital of Fudan University. Briefly, the normal middle turbinate was digested using 0.2% pronase in culture medium at 37°C for one hour for dissociation of the mucosal epithelial cells. After digestion, the dissociated cells were washed with PBS, followed by the centrifuge(400 g×5 min). The cell pallet was resuspended with culture medium (BEBM supplemented with BEGM SingleQuots) and plated on a 100 mm culture dish at 37°C for 2 hours to remove fibroblasts, myocytes, and endothelial cells. Then the harvested epithelial cells in the supernatant were grown with culture medium in a 5% CO2 incubator at 37°C. After confluence, the cells were detachment with 0.25% trypsin-0.02% EDTA and then the cells were sub-cultured in 6-well tissue culture plates.
Preparation for stimulation experiment
After reaching 80% confluence, the NCI-H292 cells were starved overnight in serum-free RPMI 1640 medium, followed by the stimulation in serum-free medium; PNEC cultured in tissue culture plates were starved overnight in BEBM medium (without BEGM SingleQuots), and subsequently stimulated in BEBM medium.
Measurement of cell viability
NCI-H292 and primary nasal epithelial cells viability were assessed 24 h after stimulation by incubating cells with Cell Counting Kit 8 according to the manufacturer's instructions.
Enzyme-linked immunosorbent (ELISA) assay
After stimulation, 1 ml of the supernatant in each well was collected; centrifuge (5000 rpm for 5 min) and the cell-free supernatants were stored at −20°C until analysis. The level of IL-6 in supernatant was analyzed using ELISA. A Standard curve was made in each plate with the highest concentration of 20000 pg/ml followed by 2-fold dilution. Each sample was measured at 1, 10, or 100 times dilution.
RNA isolation and Real-time PCR
Total RNA for each sample was isolated using trizol according to manufacturer's protocol. RNA purification was performed using nucleospin RNA II (Machery-Nagel, Germany). RNA concentration was measured using the nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE, USA). cDNA was synthesized using the MBI Fermentas first strand cDNA synthesis kit. Polymerase chain reaction was performed on Bio-Rad iCycler (Bio-Rad, Veenendaal, the Netherlands). TaqMan® primer (Roche Molecular Systems, Pleasanton, CA, USA) and probe sequences for GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was obtained from Sigma-Aldrich (Haverhill, UK). The sequences for PCR reactions are: GAPDH; sense: 5'-GAA-GGTGAA-GGT-CGG-AGT-C-3',probe:5'-Texas red-CAA-GCT-TCCCGT-TCT-CAG-CC-BHQ2-3', antisense: 5'-GAA-GAT-GGTGAT-GGG-ATT-TC-3'. For the other genes, we ordered TaqMangene expression assays from Applied Biosystems (Nieuwerkerka/dIJssel, the Netherlands) with the following IDs: LL37; Hs00189038_m1. TLR3; Hs01551077_m1. Expression changes are presented as ΔΔct, indicating the difference in threshold cycle between active sample and negative control, after correcting for the expression of the housekeeping gene.
Quantitative measurement of LL37 protein
Cells were lysed in RIPA. The protein concentration was determined using Protein Assay Solution (Beyotime, China). Equal amount of denaturation proteins were separated on a SDS-PAGE on a 12% glycine-based gel, followed by being transferred to a polyvinylidene difluoride membrane (Millipore, USA), and nonspecific binding sites were blocked. The membrane was incubated with mouse anti-human LL37 antibody (1:1000 dilution, abcam ab87701). The membrane was then incubated with the corresponding horseradish peroxidase labeled secondary goat anti-mouse IgG antibody (1:2500). Immunoreactive proteins were detected with the enhanced chemiluminescence (ECL) western blot detection system (Beyotime, China). b-actin protein was added as the endogenous reference.
Statistical analysis
Each set of results shown is representative of at least three separate experiments. Experiments were performed in triplicate and values are shown as the mean ± SD. Statistical significance was determined using the non-parametric Kruskal–Wallis test for variance. When the result was significant, the Mann–Whitney U test was performed for comparisons between groups (SPSS software). All reported P values are 2-sided, and values less than 0.05 were considered to indicate statistical significance.
HDAC inhibitors directly induce LL-37 gene expression in NCI-H292 human airway epithelial cells
To confirm the findings obtained with TSA, we tested the effect of other HDAC inhibitor, SB. Like TSA, SB used at concentrations (1 mM, 2 mM, 4 mM and 8 mM) dose dependently increased LL37 expression in the NCI-H292 cell (Figure 1C).
Our results indicate that TSA(200 nM) or SB(4 mM) stimulation for 24 h could effectively up-regulate LL37 gene expression, so, we use TSA(200 nM) or SB(4 mM) through our following experiment.
HDAC inhibitors induce cathelicidin LL-37 gene expression in human primary nasal epithelial cell
HDAC inhibitors up-regulate LL37 protein expression in NCI-H292 human airway epithelial cells but not in primary nasal epithelial cells
HDAC inhibitors suppress IL-6 production after poly(I:C) stimulation
The effect of HDAC inhibitors on TLR3 expression in airway epithelial cells
The inhibition of HDAC inhibitors on poly(I:C) induced expression of IL-6 we observed in the previous experiment could be mediated at many different levels. To explore whether some of the inhibitory effect could be upstream of the IL-6 genes we determined TLR3 expression levels as a measure of different HDAC inhibitors concentrations. Our results showed that poly(I:C) stimulation without TSA or SB increased the TLR3 expression by more than one and a half times, and in the presence of different concentrations of HDAC inhibitors, the induced expression of TLR3 gene expression was not seen significantly alternative expression(data not shown), indicating that the inhibition of HDAC inhibitors on poly(I:C) induced expression of IL-6 was not due to TLR3 expression levels.
In this study, cell viability after the stimulation was assessed by the Cell Counting Kit 8. Our data showed that the stimulation with various concentration of poly(I:C), TSA or SB had a minimal effect on cell viability.
In the present study, we have shown a complex interplay between epigenetics and aspects of the innate immune reaction in airway epithelial cells. HDAC inhibitors on one hand inhibit poly(I:C)-induced expression of IL-6, while on the other hand they directly induces LL-37 expression in NCI-H292 human airway epithelial cells. In the primary nasal epithelial cells, we found that only SB inhibited poly(I:C)-induced expression of IL-6 and that both TSA and SB could induce LL37 gene, not protein, expression. Our results indicate that epigenetic regulation plays an important, yet complicated, role in the regulation of innate immunity in airway epithelial cells.
All these observations of inhibition under unstimulated or stimulated conditions seem contrary to what one would expect for the action of an inhibitor of deacetylases. As this inhibition would lead to higher levels of (local) histone acetylation one might expect increased levels of gene activity. In our experiments only the expression of LL-37 seems to follow the expected paradigm. However, TSA and SB might act indirectly on a target gene by affecting the expression of some negative regulator only, or in combination with a positive effect on either the target gene itself or some positive regulator.
Epithelium derived antimicrobial peptide LL-37 is an important component of host defense at mucosal surfaces and exposure to TLR3 agonist is indeed able to up-regulate the expression of LL-37 in primary human corneal epithelial cells [11], just like it was in the airway epithelial cells. However, the positive effects of TSA and SB were much stronger than that of the TLR3 activator and, moreover, this activation does not require the presence of the TLR3 agonist. The positive effect of TSA and SB on the gene expression of LL-37 in airway epithelium is consistent with previous study reported by Schauber et al. that histone-deacetylase inhibitors (butyrate) induce the cathelicidin LL-37 in gastrointestinal cells. And they further demonstrated that butyrate induced expression of LL-37 was mediated by MEK-ERK signalling pathway [12]. The different expression of LL37 protein in primary nasal epithelial cells and NCI-H292 cells needs further research.
What is the mechanism underlying HDAC inhibitors induced LL37 expression? Emerging evidence indicates that HDAC inhibitors play an important role in the modulation of core histone (H3 and H4) and non-histone proteins. Butyrate and TSA were reported to induce LL37 expression via acetylation of the non-histone protein HMG-N2 and the histone protein H4 in HT-29 colon, 23132/87 gastric and HepG2 hepatoma cells [12]. LL-37 gene had potential binding sites for several transcription factors, including NF-kB and activator protein-1 [13]. Kuwano et al[14] reported sodium butyrate induced histone acetylation of the cathelicidin promoter using the chromatin immunoprecipitation (ChIP) in the human lung epithelial cell line. They also indicated that activator protein-1 plays an important role in the regulation of sodium butyrate-induced transactivation of cathelicidin promoter. In the present study, our results revealed that TSA and SB induced LL37 expression both in gene and protein levels in NCI-H292 cells, which is consistent with the previous reports. Unlike the previously reported effect of HDAC inhibitors on the LL37 expression, Schauber et al. indicated that HDAC inhibitors(TSA and butyrate) alone did not change cathelicidin transcript abundance in keratinocytes. They demonstrated that HDAC inhibition significantly amplify cathelicidin expression in keratinocytes in the presence of 1,25-Dihydroxyvitamin D3 [15]. So, we speculate that acetylation of cathelicidin promoter play an important role in LL37 expression. Our results in the nasal epithelial cells indicated that HDAC inhibitors could induce LL37 gene expression, but not the LL37 protein. These observations show that the nature of a response to histone acetylation will be cell-type and gene-specific.
The airway epithelium itself is responsible for the synthesis and release of cytokines that cause the selective recruitment, retention, and accumulation of various inflammatory cells [16, 17]. Target cells of the epithelium can respond to a variety of inflammatory mediators and cytokines. IL-6 is a multifunctional cytokine that regulates the immune response, the acute phase response and inflammation. IL-6 is involved in the pathogenesis of lung diseases such as asthma and chronic obstructive pulmonary disease [18]. Our results demonstrated a suppressive effect on IL-6 expression in TSA-exposed airway epithelial cells. These observation are in line with those of Grabiecet al[15] that also reported that TSA significantly reduced the production of IL-6 after exposure to multiple stimuli, including poly(I:C), in fibroblast-like synoviocyte and macrophages. Although this group did not investigate TLR3 expression they indicated that the inhibitory effect of TSA was a consequence of accelerated mRNA decay. Our observation of a direct effect of TSA on TLR3 is supported by similar observations in human microglia and astrocytes in their response to poly(I:C) [19].
In addition to the expression of individual genes, the global character of the action of TSA is probably also the reason for its ability to suppress cell growth by inducing cell cycle arrest and to promote differentiation of normal and transformed cells [20]. Increasing evidence suggests that HDAC inhibitors are indeed potent anti-inflammatory and immunomodulatory agents [21, 22].
In summary, our results indicate that regulation of histone acetylation and chromatin remodelling plays a complex role in innate immune responses in airway epithelium.
Funding source
This work was supported by the Interuniversity Attraction Poles Programme (IUAP) –Belgian state – Belgian Science Policy P6/35 and Science and Technology Commission of Shanghai Municipality (10XD1401000).
We thank Silvia, Danielle, Esther, and Jing Hou for technical assistance. This study was supported by the Interuniversity Attraction Poles Programme (IUAP) – Belgian state – Belgian Science Policy P6/35 and Science and Technology Commission of Shanghai Municipality (10XD1401000).
Authors' Affiliations
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