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2019-02-23T01:45:17Z http://journal.waocp.org/?_action=export&rf=summon&issue=3533 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 'Drawing' a Molecular Portrait of CIN and Cervical Cancer: a Review of Genome-Wide Molecular Profiling Data In this review we summarize the results of studies employing high-throughput methods of profiling ofHPV-associated cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancers at key intracellularregulatory levels to demonstrate the unique identity of the landscape of molecular changes underlying thisoncopathology, and to show how these changes are related to the 'natural history' of cervical cancer progressionand the formation of clinically significant properties of tumors. A step-wise character of cervical cancer progressionis a morphologically well-described fact and, as evidenced by genome-wide screenings, it is indeed the consistentchange of the molecular profiles of HPV-infected epithelial cells through which they progressively acquire thephenotypic hallmarks of cancerous cells. In this sense, CIN/cervical cancer is a unique model for studying thedriving forces and mechanisms of carcinogenesis. Recent research has allowed definition of the whole-genomespectrum of both random and regular molecular alterations, as well as changes either common to processes ofcarcinogenesis or specific for cervical cancer. Despite the existence of questions that are still to be investigated,these findings are of great value for the future development of approaches for the diagnostics and treatment ofcervical neoplasms. Cervical cancer-CIN-HPV-molecular profile-genome-signature 2015 11 01 4477 4487 http://journal.waocp.org/article_31111_1c7c93033e2bd957fc64a3f3ca639665.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Current Status and Future Perspectives of Sonodynamic Therapy and Sonosensitiers The precaution and treatment for cancer become inevitable with the rising of morbidity and mortality. Inthis article, a promising new methodology for cancer treatment, sono-dynamic therapy (SDT) was introduced.In addition, we extensively reviewed the molec-ular mechanisms of SDT killing cancer and summarized theclassification of sonosensi-tizers. At the same time, research progress of SDT indicates that it is possible to becomea developing field for cancer treatment in clinical application. cancer sonodynamic therapy sonosensitizer 2015 11 01 4489 4492 http://journal.waocp.org/article_31112_3d7afe96a56b0009f79b548ff67ebda1.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Are PIK3CA Mutation and Amplification Associated with Clinicopathological Characteristics of Gastric Cancer? Alterations in mitochondrial DNA (mtDNA) have been studied in various cancers. However, the clinical valueof mtDNA copy number (mtCN) alterations in gastric cancer (GC) is poorly understood. In the present study,we investigated whether alterations in mtCNs might be associated with clinicopathological parameters in GCcases. mtCN was measured in 109 patients with GC by real-time PCR. Then, correlations with clinicopathologicalcharacteristics were analyzed. mtCN was elevated in 64.2% of GC tissues compared with paired, adjacent, noncanceroustissue. However, the observed alterations in mtCN were not associated with any clinicopathologicalcharacteristics, including age, gender, TN stage, Lauren classification, lymph node metastasis, and depth ofinvasion. Moreover, Kaplan-Meier survival curves revealed that mtCN was not significantly associated with thesurvival of GC patients. In this study, we demonstrated that mtCN was not a significant marker for predictingclinical characteristics or prognosis in GC. Gastric cancer Mitochondrial DNA copy number Prognosis 2015 11 01 4493 4496 http://journal.waocp.org/article_31113_c497de1be961029f4a9aa98b5fe72833.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Comparative Study of Carcinoembryonic Antigen Tumor Marker in Stomach and Colon Cancer Patients in Khyber Pakhtunkhwa Background: Due to the increase in morbidity and mortality rate, cancer has become an alarming threat tothe human population worldwide. Since cancer is a progressive disorder, timely diagnosis would be helpful toprevent/stop cancer from progressing to severe stage. In Khyber Pakhtunkhwa, Pakistan, most of the time, tumorsare diagnosed with endoscopy and biopsy; therefore rare studies exist regarding the diagnosis of gastrointestinal(GIT) carcinomas based on tumor markers, especially CEA.
Objectives: This study made a comparative analysisof CEA in admitted hospitalized stomach and colon cancer patients diagnosed as GIT with biopsy. Materials and
Methods: In this study, a total of 66 cases were included. The level of CEA was determined in the blood of thesepatients using ELISA technique.
Results: Out of 66 patients, the level of CEA was high in 59.1% of the total,60.7% in colon cancer patients and 57.9 % in stomach cancer patients. Moreover, the incidence of colorectaland stomach cancer was greater in males as compared to females. Patients were more of the age group of 40-60 and the level of CEA was comparatively higher in patients (51.5%) with histology which was moderatelydifferentiated, than patients with well differentiated and poorly differentiated tumor histology.
Conclusions:CEA level was high in more than 50% of the total patients. Moreover, CEA exhibited higher sensitivity for colonthan stomach cancer. Gastrointestinal tumors CEA Khyber Pakhtunkhwa enzyme linked immunosorbant assay 2015 11 01 4497 4502 http://journal.waocp.org/article_31114_d54493d3280eae26dbdc6e2ea5f78948.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Relations of Serum Visfatin and Resistin Levels with Endometrial Cancer and Factors Associated with its Prognosis Background: The aims of this study were compare the serum visfatin and resistin levels between endometrialcancer (EC) patients and controls and evaluate their power to predict prognosis. Materials and
Methods: Thisprospective study was conducted between March 2013 to June 2014 on the Gynecologic Oncology Departmentof the University of Selcuk, Konya, Turkey. A total of 42 EC patients and 42 controls were included and assessedfor differences in serum visfatin and resistin levels, along with prognostic factors.
Results: Endometrial cancerpatients had significantly higher visfatin levels than control s (p: 0.011), associated with deep myometrial invasion(p: 0.019). In contrast the serum level of resistin did not significantly differ between EC patients and controls (p:0.362). However, high resistin level in EC patients was associated with increase lymph node metastasis (p: 0.009).On logistic regression analysis, we found that serum visfatin elevation was associated with risk of myometrialinvasion (OR: 1,091; 95%CI: 1.021- 1.166; p: 0.010) and serum resistin with risk of lymph node metastasis(OR: 1.018; 95%CI: 1.000- 1.035; p: 0.046). For myometrial invasion prediction, a serum visfatin level greaterthan 26.8 ng/mL demonstrated a sensitivity and specificity of 66.6 % and 96.4%, respectively. For lymph nodemetastasis prediction, the best cut-off for serum resistin level was 599ng/mL. A serum resistin level greater thanthis demonstrated a sensitivity and specificity of 87.5% and 77.1%, respectively.
Conclusions: Our data suggestthat serum visfatin is elevated in patients with EC and serum visfatin and resistin levels could be used to predictthe risk of advance stage lesions. Visfatin Resistin Endometrial cancer adipocytokine 2015 11 01 4503 4508 http://journal.waocp.org/article_31115_ec4037623feda55f6b2a6bb88287fcc4.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 miR-9 Modulates Osteosarcoma Cell Growth by Targeting the GCIP Tumor Suppressor Osteosarcoma is the most common primary bone tumor in humans, especially in childhood. However, thegenetic etiology for its pathogenesis remains elusive. It is known that microRNAs (miRNAs) are involved in thedevelopment of tumor progression. Here we show that microRNA-9 (miR-9) is a potential oncogene upregulatedin osteosarcoma cells. Knockdown of miR-9 in osteosarcoma resulted in suppressed colony formation and cellproliferation. Further study identified GCIP, a Grap2 and cyclin D interacting protein, as a direct target of miR-9. In addition, GCIP overexpression activated retinoblastoma 1 (Rb) and suppressed E2F transcriptional targetexpression in osteosarcoma cells. Moreover, GCIP depletion reversed miR-9 knockdown induced colony formationand cell proliferation suppression. In sum, these results highlight the importance of miR-9 as an oncogene inregulating the proliferation of osteosarcoma by directly targeting GCIP and may provide new insights into thepathogenesis of osteosarcoma. miR-9 osteosarcoma GCIP Proliferation tumor suppressor 2015 11 01 4509 4513 http://journal.waocp.org/article_31116_d4034ff37d1f708c53e16498e6a914c2.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Ki-67 Can Predict the Response to the Gemcitabine, Oxaliplatin And L-asparaginase Regimen (GELOX) and Prognosis in Patients with Nasal Natural Killer/T-cell Lymphoma GELOX (gemcitabine, oxaliplatin and L-asparaginase) regimen showed an impressive result in our previousstudy, but the effect of this new regimen is still dissatisfying for some patients, so it is necessary to identify whichpatients will benefit from this regimen. A total of fifty-one cases with nasal natural killer/T-cell lymphoma receivinginitial GELOX chemotherapy were enrolled in this study. The ki-67 expression detected by immunohistochemistry(IHC) in the specimens ranged from 10% to 90%, with a median value of 70%, so cases higher than the medianvalue (≥70%) were defined as high ki-67 expression, and the others were designated as low ki-67 expression.The response rate had no statistical difference between low ki-67 expression group and high ki-67 expressiongroup (P=0.291) though the value in the former group was relatively high. After a median follow-up of 18.03months, the 3-year progression-free survival (PFS) for patients with low ki-67 expression was significantly higherthan those with high ki-67 expression (83.8% vs. 47.9%, P=0.038). In the stage I/II subgroup, 3-year PFS andoverall survival (OS) were statistically higher in the patients with low ki-67 expression than those with high ki-67 expression. Multivariate analysis revealed high ki-67 expression was an independent prognostic factor forPFS. These results suggest that low ki-67 expression can predict a good response of GELOX in these patients,and the combination of ki-67 expression and early stage is helpful to identify an excellent prognosis subgroupfrom patients receiving GELOX in this disease. Ki-67 Gemcitabine Oxaliplatin L-asparaginase natural killer/T-cell lymphoma 2015 11 01 4515 4520 http://journal.waocp.org/article_31117_df9852e93112e36b50db8720cd8c6796.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Role of exon 7 PTEN Gene in Endometrial Carcinoma Background: Endometrial carcinoma is the most common malignant tumor of the female genital tract andthe fourth most common cancer in Iranian women after breast, colorectal and lung cancers. Various geneticalterations appear to be early events in the pathogenesis of endometrial carcinoma and it seems that PTEN isthe most commonly mutated gene in the endometrioid subtype. The aim of the present study was to investigatethe correlation between mutations in exon 7 of PTEN gene and endometrial carcinoma. Materials and
Methods:Seventy-five patients with endometrial carcinoma and 75 females whose underwent hysterectomy for non tumoralindication were selected for evaluation of PTEN mutations in exon 7 by PCR-SSCP and sequencing. Correlationsbetween the frequency and type of mutation and the pathologic findings of the cancer (tumor subtype, stageand grade) were assessed.
Results: All of the samples were obtained from Iranian patients. 60 % (45 cases) ofthe tumors were endometriod and 40% (30 cases) were of serous type. The grade distributions of the 75 casesaccording to the FIGO staging system were as follows: low grade, 20 cases; high grade 55 cases, low stage, 41cases; high stage 34 cases. For exon 7 of the PTEN gene, the analysis showed that there were no mutations in ourcases.
Conclusions: Our findings in the present study suggest that exon 7 of PTEN does not play any significantrole in the development of endometrial carcinoma in Iranian cases. endometrial carcinoma PTEN exon 7 mutations 2015 11 01 4521 4524 http://journal.waocp.org/article_31118_e45676171ac6c1d1ff51844f20b5130b.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Association between MTHFR C677T Polymorphism and Risk of Prostate Cancer: Evidence from 22 Studies with 10,832 Cases and 11,993 Controls Background: The MTHFR C677T polymorphism is a genetic alteration affecting an enzyme involved infolate metabolism, but its relationship to host susceptibility to prostate cancer remains uncertain. The aim ofthis study was to investigate the association between MTHFR C677T polymorphism and prostate cancer byperforming a meta-analysis. Materials and
Methods: Pubmed and Web of Science databases were searched forcase-control studies investigating the association between MTHFR C677T polymorphism and prostate cancer.Odds ratios (OR) and 95% confidence intervals (95%CI) were used to assess any link.
Results: A total of 22independent studies were identified, including 10,832 cases and 11,993 controls. Meta-analysis showed thatthere was no obvious association between MTHFR C677T polymorphism and risk of prostate cancer underall five genetic models. There was also no obvious association between MTHFR C677T polymorphism andrisk of prostate cancer in the subgroup analyses of Caucasians. In contrast, MTHFR C677T polymorphismwas associated with increased risk for prostate cancer in Asians with the allele model (C vs G: OR=1.299, 95%CI =1.121-1.506, P=0.001, Pheterogeneity =0.120, I2=45%), additive genetic model (CC vs TT: OR =1.925, 95 %CI= 1.340-2.265, P=0.00, Pheterogeneity =0.587, I2=0.00%), recessive model (CC vs TT+TC: OR= 1.708, 95 % CI=1.233-2.367, P=0.001, Pheterogeneity =0.716, I2=0.00%), and heterozygote genetic model (CT vs TT: OR=2.193, 95% CI =1.510-3.186, P=0.000, Pheterogeneity =0.462, I2=0.00%).
Conclusions: These results suggest that the MTHFRC677T polymorphism does not contribute to the risk of prostate cancer from currently available evidence inpopulations overall and Caucasians. However, the meta analysis indicates that it may play a role in prostatecancer development in Asians. MTHFR C677T Polymorphism Prostate Cancer Meta Analysis 2015 11 01 4525 4530 http://journal.waocp.org/article_31119_cff5064a349e7526c76b3fb24348fd93.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Effects of Offspring-Related Characteristics on Depressive Disorder among Cancer Patients and Survivors
Objective: To investigate the influence of offspring-related characteristics on the prevalence of depressivedisorders among cancer patients and those who survived cancer for at least 45 years. Materials and
Methods:Data were obtained from the Korean Longitudinal Study of Aging (KLoSA). To investigate the associationbetween offspring and depressive disorder among cancer patients and survivors, we analyzed data from 292cancer patients and survivors drawn from a total subject pool of 16,613 individuals at baseline.
Results: Accordingto our results, the odds ratio (OR) for subjects with five or more offspring developing depressive disorder was-0.794 (p-value: 0.039, SE: 0.329) compared with that of those with two offspring. In addition, the adjustedeffect of the number of male and female offspring on the presence of depressive disorder showed that the ORfor those with three or more female offspring for developing depressive disorder was -0.958 lower (SE: 0.305,p-value: 0.012) than it was for those with no female offspring.
Conclusions: This article provides evidence foran association between offspring-related characteristics and depressive disorders among cancer patients andsurvivors. Therefore, offspring may be important contributors to the emotional status of cancer patients andsurvivors. Further study should precisely need to measure depressive disorders because of self-reported data. Offspring cancer Depressive disorder Korea 2015 11 01 4531 4536 http://journal.waocp.org/article_31120_eb4d275037d56608a6c5356366306a94.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 OK-432 Suppresses Proliferation and Metastasis by Tumor Associated Macrophages in Bladder Cancer OK-432, a Streptococcus-derived anticancer immunotherapeutic agent, has been applied in clinic for manyyears and achieved great progress in various cancers. In the present study, we investigated its anticancer effecton bladder cancer through tumor associated macrophages (TAMs). MTS assay validated OK-432 could inhibitproliferation in both T24 and EJ bladder cell lines. OK-432 also induced apoptosis of bladder cancer cells in vitro.Consequently, we demonstrated that OK-432 could suppress the bladder cancer cells migration and invasion byaltering the EMT-related factors. Furthermore, using SD rat model, we revealed that OK-432 inhibited tumorgrowth, suppressed PCNA expression and inhibited metastasis in vivo. Taken together, these findings stronglysuggest that OK-432 inhibits cell proliferation and metastasis through inducing macrophages to secret cytokinesin bladder cancer. OK-432 bladder cancer Proliferation Apoptosis TAMs 2015 11 01 4537 4542 http://journal.waocp.org/article_31121_9fcfa5626aeb3f155e62e3504fb45a63.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 The Effects of ASMase Mediated Endothelial Cell Apoptosis in Multiple Hypofractionated Irradiations in CT26 Tumor Bearing Mice Background: To investigate the effects of ASMase mediated endothelial cell apoptosis in multiplehypofractionated irradiations in CT26 tumor bearing mice. Materials and
Methods: Thirty-five CT26 tumorbearing mice were subjected to single ionizing radiation (IR) of 0, 3, 6, 9, 12, 15, 18 Gy. Eight hours after IR,the mice were sacrificed and tumor tissues were used for CD31 immunohistochemistry staining, TUNEL andCD31 double staining, ASMase activity assay. Then 6 and 12 Gy were chosen for multiple hypofractionated IRexperiments according to the above results. Each time after IR, 5 mice were sacrificed and assayed as above.
Results: The ASMase activities were increased significantly after a single IR of 12 Gy or higher which wasaccompanied with remarkable increased endothelial cell apoptosis and decreased MVD. For 6 Gy which was nothigh enough to trigger ASMase activation, after 2 or more times of IR, the ASMase activities were significantlyincreased accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. While for12 Gy, after 2 or more times of IR, the ASMase activities and endothelial cell apoptosis rates were maintainedwithout remarkable increase; however, the MVD was significantly decreased. What's more, the cancer cellapoptosis rates were significantly increased after multiple IR for both 6 Gy and 12 Gy.
Conclusions: ASMasemediated endothelial cell apoptosis may play an important role in the process of multiple hypofractionated IRfor CT26 colorectal carcinoma. ASMase Endothelial cell hypofractionated irradiations CT26 tumor bearing mice 2015 11 01 4543 4548 http://journal.waocp.org/article_31122_71c1d4d6ed683347d3ba9b4564b4f835.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Endothelial Cell Proliferation and Vascular Endothelial Growth Factor Expression in Primary Colorectal Cancer and Corresponding Liver Metastases Background: . Colorectal carcinoma (CRC) is one of the major causes of cancer death worldwide. Data fromthe literature indicate differences between the proliferation rate of endothelial cells relative to the morphologygrowth type, possibly due to origin of specimens (autopsy material, surgery fragments) or quantification methods.Vascular endothelial growth factor (VEGF) is a factor that stimulates the proliferation of endothelial cells. It isexpressed in more than 90% of cases of metastatic CRC. Aim: The aim of this study was to evaluate the endothelialcell proliferation and VEGF expression in primary tumors and corresponding liver metastases. Materials and
Methods: Our study included 24 recent biopsies of primary tumors and corresponding liver metastases of CRCcases. CD34/ Ki67 double immunostaining and RNA scope assay for VEGF were performed.
Results: In theprimary tumors analysis of VEGFmRNA expression indicated no significant correlation with differentiationgrade, proliferative and non-proliferative vessels in the intratumoral and peritumoral areas. In contrast, in thecorresponding liver metastases, VEGFmRNA expression significantly correlated with the total number of nonproliferativevessels and total number of vessels. CD34/ Ki67 double immunostaining in the cases with poorlydifferentiated carcinoma indicated a high number of proliferating endothelial cells in the peritumoral area anda low number in the intratumoral area for the primary tumor. Moderately differentiated carcinomas of colonshowed no proliferating endothelial cells in the intratumoral area in half of the cases included in the study, forboth, primary tumor and liver metastasis. In well differentiated CRCs, in primary tumors, a high proliferationrate of endothelial cells in the intratumoral area and a lower proliferation rate in the peritumoral area were found.A low value was found in corresponding liver metastasis.
Conclusions: The absence of proliferative endothelialcells in half of the cases for the primary tumors and liver metastases in moderately differentiated carcinomasuggest a vascular mimicry phenomenon. The mismatch between the total number of vessels and endothelialproliferation in primary tumors indicate that a functional vascular network is already formed or the existenceof some mechanisms influenced by other angiogenic factors. colon carcinoma endothelial cell proliferation Metastasis 2015 11 01 4549 4553 http://journal.waocp.org/article_31123_12d3aee5fd4108f3b2809f8959ce891c.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Participation of the Women Covered by Family Physicians in Breast Cancer Screening Program in Kerman, Iran Background: Mammography screening is a method for reducing breast cancer mortality in women over 40years old . A participation rate of at least 70% is a prerequisite for screening programs. This study aimed atdetermining the participation rate of women in breast cancer screening in Iran. Materials and
Methods: Thestudy population in this prospective research consisted of 35 to 69 years old women in the villages and townsKerman District, in 2013. The data were collected by a well-validated risk assessment questionnaire. Thequestionnaires were completed with the help of health workers and technicians in the health centers, who weretrained on breast cancer screening program.
Results: As a whole, 19,651 women were invited to complete thequestionnaire, of whom 15,794 women (80.37%) completed it. In the urban region, of 3150 eligible women 2728women (86.60%) participated in the study. The acceptance rates for mammography in rural and urban regionswere 34.95% and 8.75%, respectively.
Conclusions: Finally, 3.8% and 16.34% of 35 to 69 years old women inthe urban regions were mammographed, respectively.
Conclusion: The low participation of eligible women inbreast cancer screening program alerts us against including the program in the health insurance package. 2015 11 01 4555 4561 http://journal.waocp.org/article_31124_8ada5febe2debadd6894e062195a30ca.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Smoking among Secondary School Students in Kota Tinggi, Johor, Malaysia - Findings from a Cross-Sectional Study Background: Smoking is a learnt behavior during adolescence and understanding the factor/s associatedwith smoking will assist in identifying suitable measures in combating the rising prevalence of smoking amongadolescents. This research aimed to identify the factor/s associated with smoking among form four students inKota Tinggi, Johor. Multistage sampling was used to select a representative sample of students in 2008 and datawere collected using a self-administered validated questionnaire. This study revealed that the overall smokingprevalence was 19.0% with a significantly higher proportion of male smokers (35.8%) as compared to females(3.15%). Adolescents who were male (aOR 6.6, 95%CI 2.61-16.4), those who had peer/s who smoked (aOR 4.03,95% CI 1.31-12.4), and those who studied in rural areas and Felda Settlements ( aOR 4.59, 95 CI 1.11-18.0; aOR9.42, 95%CI 3.91-29.1) were more likely to smoke in the past one week. On the other hand, adolescents withbetter knowledge on the hazards of smoking and negative attitudes towards smoking were less likely to smoke(aOR 0.51, 95%CI 0.37-0.72; aOR 0.67, 95%CI 0.46-0.99). Future promotional and interventional programmeson smoking should be considered and the above identified risk factors integrated to reduce smoking prevalenceamong students of school-going ages in Kota Tinggi. Johor. Adolescent smoking weekly smoker intrapersonal interpersonal Johor 2015 11 01 4563 4570 http://journal.waocp.org/article_31125_0bafb8188aadf57f8d05a943433830c1.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 The Inhibition Effect of Triptolide on Human Endometrial Carcinoma Cell Line HEC-1B: a in vitro and in vivo Studies Background: To investigate the inhibitory effect and the underlying mechanism of triptolide on culturedhuman endometrial carcinoma HEC-1B cells and corresponding xenograft. Materials and
Methods: For in vitrostudies, the inhibition effect of proliferation on HEC-1B cell by triptolide was determined by MTT assay; cellcycle and apoptosis of the triptolide-treated and untreated cells were detected by flow cytometry. For in vivostudies, a xenograft tumor model of human endometrial carcinoma was established using HEC-1B cells, thenthe tumor-bearing mice were treated with high, medium, and low-dose (8 μg, 4 μg and 2 μg/day) triptolide orcisplatin at 40 μg/day or normal saline as control. The mice were treated for 10-15 days, during which body weightof the mice and volume of the xenograft were weighted. Then expression of Bcl-2 and vascular endothelial growthfactor (VEGF) was analyzed by SABC immunohistochemistry.
Results: Cell growth was significantly inhibitedby triptolide as observed by an inverted phase contrast microscope; the results of MTT assay indicated thattriptolide inhibits HEC-1B cell proliferation in a dose and time-dependent manner; flow cytometry showed thatlow concentration (5 ng/ml) of triptolide induces cell cycle arrest of HEC-1B cells mainly at S phase, while higherconcentration (40 or 80 ng/ml) induced cell cycle arrest of HEC-1B cells mainly at G2/M phase, and apoptosisof the cells was also induced. High-dose triptolide showed a similar tumor-inhibitory effect as cisplatin (-50%);high-dose triptolide significantly inhibited Bcl-2 and VEGF expression in the xenograft model compared tonormal saline control (P<0.05).
Conclusions: triptolide inhibits HEC-1B cell growth both in vitro and in mousexenograft model. Cell cycle of the tumor cells was arrested at S and G2/M phase, and the mechanism may involveinduction of tumor cell apoptosis and inhibition of tumor angiogenesis. Uterine cancer-triptolide-VEGF- Bcl-2 2015 11 01 4571 4576 http://journal.waocp.org/article_31126_d80e212efa6e6608103f990aea017a16.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Breast Cancer Inpatients Undergoing Mastectomy from a Hospital in Guangzhou, China: A Retrospective Analysis 2004-2013 Background: To explore the hospitalizations of breast cancer patients undergoing mastectomy, and toprovide a basis for management, clinical prevention and treatment. Materials and
Methods: We conductedan investigation by means of the retrospective survey and the medical records retrieval system, and made outthe data of patients suffered from breast cancer in a hospital in Guangzhou from 2004 to 2013, including age,medical payment methods, pathological type, treatment, treatment results, complications, hospitalization days,cost and so on.
Results: The average age of the inpatients was 50.14 years old. The main histologic types wereinfiltrating duct carcinoma (88.06%). The main surgery was modified radical mastectomy (80.41%). The curerate was 90.80% during the 10 years. The main medical payment method was self-paying (57.28%). The averagehospital stay was 13.51 days, and average hospitalization cost was RMB 23,083.66 yuan, proportion of drug feesup to 39.70%. Postoperative complication rate was 0.79%. The self-paying group was with the highest proportionof drug fees (P<0.05), while the free medical service group was with the longest hospitalization days (P<0.05).
Conclusions: The payment methods significantly affected the proportion of drug fees and hospitalization days.The therapeutic effect was satisfactory with less complications and reasonable proportion of drug fees in ourhospital. breast cancer hospital-based Surgery mastectomy Hospitalization cost payment methods 2015 11 01 4577 4581 http://journal.waocp.org/article_31127_2646e9374afe63c9fb0ef95e1738b1c4.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Prognostic Value of a CYP2B6 Gene Polymorphism in Patients with Acute Myeloid Leukemia Background: The objectives of this study aimed to detect a CYP2B6 polymorphism in de novo cases of acutemyeloid leukemia patients and identify any role in disease progression and outcome. Materials and
Methods:DNA was isolated from peripheral blood of 82 newly diagnosed acute myeloid leukemia cases and the CYP2B6G15631T gene polymorphism was assayed by PCR restriction fragment length polymorphism (PCR-RFLP).
Results: The frequency of the GG genotype (wild type) was 48 (58.5%) and that of the mutant type T allele was 34(41.9%). GT genotype heterozygous variants were found in 28 (34%), and TT genotype homozygous variants in6 (7.3%) cases. We found no significant association between the CYP2B6 G15631T polymorphism and completeresponse (CR) (p-value=0.768), FAB classification (p-value=0.51), cytogenetic analysis (p-value=0.673), and overallsurvival (p-value=0.325). Also, there were no significant links with early toxic death (p-value=0.92) or progressionfreesurvival (PFS) (p-value=0.245).
Conclusions: Our results suggest that the CYP2B6 polymorphism has norole in disease progression, therapeutic outcome, patient free survival, early toxic death and overall survival inacute myeloid leukemia patients. CYP2B6 G15631T AML Prognosis PCR/RFLP 2015 11 01 4583 4587 http://journal.waocp.org/article_31128_8bdd65df54edbe4400ecb44c2c661b4d.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Mutation Analysis of the Dimer Forming Domain of the Caspase 8 Gene in Oral Submucous Fibrosis and Squamous Cell Carcinomas Background: Missense and frame-shift mutations within the dimer forming domain of the caspase 8 genehave been identified in several cancers. However, the genetic status of this region in precancerous lesions, likeoral submucous fibrosis (OSMF), and well differentiated oral squamous cell carcinomas (OSCCs) in patientsfrom southern region of India is not known, and hence the present study was designed to address this issue.Materials and
Methods: Genomic DNA isolated from biopsy tissues of thirty one oral submucous fibrosis andtwenty five OSCC samples were subjected to PCR amplification with intronic primers flanking exon 7 of thecaspase 8 gene. The PCR amplicons were subsequently subjected to direct sequencing to elucidate the status ofmutation.
Results: Sequence analysis identified a frame-shift and a novel missense mutation in two out of twentyfive OSCC samples. The frame-shift mutation was due to a two base pair deletion (c.1225_1226delTG), whilethe missense mutation was due to substitution of wild type cysteine residue with phenylalanine at codon 426(C426F). The missense mutation, however, was found to be heterozygous as the wild type C426C codon was alsopresent. None of the OSMF samples carried mutations.
Conclusions: The identification of mutations in OSCClesions but not OSMF suggests that dimer forming domain mutations in caspase 8 may be limited to malignantlesions. The absence of mutations in OSMF also suggests that the samples analyzed in the present study maynot have acquired transforming potential. To the best of our knowledge this is the first study to have exploredand identified frame-shift and novel missense mutations in OSCC tissue samples. Caspase 8 mutation in oral carcinoma loss of function of caspase 8 caspase 8 mutation in cancer 2015 11 01 4589 4592 http://journal.waocp.org/article_31129_b36a2ea8f3b1b13f133d3742a15f6436.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Risk factors for Opisthorchis viverrini Infection in Nong Khai Province, Thailand Background: Opisthorchis viverrini (OV) infection is the main risk factor for cholangiocarcinoma and is oftenfound in Northeastern Thailand. The prevalence of OV infection and the incidence of cholangiocarcinoma aremajor public health problems in this region.
Objectives: The objectives of this study were to identify factorsassociated with OV infection among people in Nong Khai Province in order to develop a prevention programmein the community. Materials and
Methods: The data were collected in July 2013. Stool specimens were examinedfor intestinal parasites within hours after collection using a normal saline wet preparation and the modified Kato-Katz technique. A case-control study was conducted to collect information about demographic data, the habit ofeating unsafely prepared fish, the safe disposal of waste food, and the practice of defaecating in fields. Structuredquestionnaires were used to interview 351 participants (117 cases and 234 controls) in a random selection of 30villages across Nong Khai Province. Multiple logistic regression was used to identify risk factors for OV infection.
Results: In the multivariate analysis, the results showed that the factors which had a statistically significantassociation with OV infection were the habit of consuming unsafely prepared fish (ORadj=5.17, 95%CI=2.49-10.74), the similar habit of family members (ORadj=3.25, 95%CI=1.63-6.49) , a history of O. viverrini infection(ORadj=5.64, 95%CI=2.10-15.18), a history of taking praziquantel (ORadj=5.66, 95%CI=3.11-10.29), and theunsafe disposal of waste food (ORadj=2.1, 95%CI=1.10-3.80).
Conclusions: The findings of this study highlightthe features on which a community programme should focus in order to reduce the prevalence of opisthorchiasisand incidence of bile duct cancer. Opisthorchis viverrini - risk factor - Nong Khai Thailand 2015 11 01 4593 4596 http://journal.waocp.org/article_31130_9a329cae94e3a793ec62d2a0bf91c2b5.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Contribution of Macrophage Migration Inhibitory Factor -173G/C Gene Polymorphism to the Risk of Cancer in Chinese Population Background: Macrophage migration inhibitory factor (MIF) -173G/C (rs755622) gene polymorphism hasbeen associated with cancer risk. Previous studies have revealed that MIF -173G/C gene polymorphism mayincrease cancer in the Chinese population, while results of individual published studies remain inconsistentand inconclusive.We performed this meta-analysis to derive a more precise estimation of the relationship.Materials and
Methods: We conducted a search on PubMed, Embase, MEDLINE, Cochrane Library ,ChineseNational Knowledge Infrastructure (CNKI), Wanfang, Weipu on Dec 31, 2014.Odds ratio (OR) and 95%confidence interval (95% CI) were used to assess the association. A total of eight studies including 2,186 casesand 2,285 controls were involved in this meta-analysis.
Results: The pooled results indicated the significantassociation between MIF −173G/C polymorphism and the risk of cancer for Chinese population (CC + CGvs GG: OR=1.14, 95%CI=1.02-127, pheterogeneity<0.01; P =0.023; CC vs CG+GG: OR=1.12, 95%CI=1.02-1.23, pheterogeneity< 001; P =0.017;CC vs GG: OR=1.18, 95%CI=1.04-1.33, pheterogeneity<001; P =0.008;CG vs GG:OR=1.03, 95%CI=0.91-1.15, pheterogeneity<001; P =0.656; C vs G:OR=1.24, 95%CI=1.14-1.25,pheterogeneity<001; P <001). Subgroup analysis showed that in patients with "solid tumors", heterogeneitywas very large (OR=0.94,95%CI=0.83-1.06,pheterogeneity=0.044; p=0.297). Within "non-solid tumors", theassociation became even stronger (OR=6.62, 95 % CI=4.32-10.14, pheterogeneity<0.001; p <0.001).
Conclusions:This study suggested that MIF −173G/C gene polymorphism may increase increase cancer in the Chinesepopulation.Furthermore, more larger sample and representative population-based casees and well-matchedcontrols are needed to validate our results. Macrophage migration inhibitory factor (MIF) cancer Polymorphism Chinese 2015 11 01 4597 4601 http://journal.waocp.org/article_31131_d88c23a3c9cc519a9f9eea6e36c7e5ff.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Serum Tumor Marker Levels might have Little Significance in Evaluating Neoadjuvant Treatment Response in Locally Advanced Breast Cancer Background: To determine the potential value of serum tumor markers in predicting pCR (pathologicalcomplete response) during neoadjuvant chemotherapy. Materials and
Methods: We retrospectively monitoredthe pro-, mid-, and post- neoadjuvant treatment serum tumor marker concentrations in patients with locallyadvanced breast cancer (stage II-III) who accepted pre-surgical chemotherapy or chemotherapy in combinationwith targeted therapy at Fudan University Shanghai Cancer Center between September 2011 and January 2014and investigated the association of serum tumor marker levels with therapeutic effect. Core needle biopsy sampleswere assessed using immunohistochemistry (IHC) prior to neoadjuvant treatment to determine hormone receptor,human epidermal growth factor receptor 2(HER2), and proliferation index Ki67 values. In our study, therapeuticresponse was evaluated by pCR, defined as the disappearance of all invasive cancer cells from excised tissue(including primary lesion and axillary lymph nodes) after completion of chemotherapy. Analysis of variance ofrepeated measures and receiver operating characteristic (ROC) curves were employed for statistical analysisof the data.
Results: A total of 348 patients were recruited in our study after excluding patients with incompleteclinical information. Of these, 106 patients were observed to have acquired pCR status after treatment completion,accounting for approximately 30.5% of study individuals. In addition, 147patients were determined to be Her-2positive, among whom the pCR rate was 45.6% (69 patients). General linear model analysis (repeated measuresanalysis of variance) showed that the concentration of cancer antigen (CA) 15-3 increased after neoadjuvantchemotherapy in both pCR and non-pCR groups, and that there were significant differences between thetwo groups (P=0.008). The areas under the ROC curves (AUCs) of pre-, mid-, and post-treatment CA15-3concentrations demonstrated low-level predictive value (AUC=0.594, 0.644, 0.621, respectively). No significantdifferences in carcinoembryonic antigen (CEA) or CA12-5 serum levels were observed between the pCR andnon-pCR groups (P=0.196 and 0.693, respectively). No efficient AUC of CEA or CA12-5 concentrations wereobserved to predict patient response toward neoadjuvant treatment (both less than 0.7), nor were differencesbetween the two groups observed at different time points. We then analyzed the Her-2 positive subset of ourcohort. Significant differences in CEA concentrations were identified between the pCR and non-pCR groups(P=0.039), but not in CA15-3 or CA12-5 levels (p=0.092 and 0.89, respectively). None of the ROC curves showedunderlying prognostic value, as the AUCs of these three markers were less than 0.7. The ROC-AUCs for theCA12-5 concentrations of inter-and post-neoadjuvant chemotherapy in the estrogen receptor negative HER2positive subgroup were 0.735 and 0.767, respectively. However, the specificity and sensitivity values were at oddswith each other which meant that improving either the sensitivity or specificity would impair the efficiency ofthe other.
Conclusions: Serum tumor markers CA15-3, CA12-5, and CEA might have little clinical significancein predicting neoadjuvant treatment response in locally advanced breast cancer. serum tumor markers Neoadjuvant chemotherapy PCR prognostic function 2015 11 01 4603 4608 http://journal.waocp.org/article_31132_a03f9ea632f1e598df0848ede23cf26c.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Outcome of Childhood Acute Lymphoblastic Leukemia Treated Using the Thai National Protocols Background: In recent decades, the prognosis for childhood leukemia has improved, especially for acutelymphoblastic leukemia (ALL). In Thailand, though, the survival rate for ALL is unimpressive. In 2006, standardnational protocols for childhood leukemia treatment were implemented. We herein report the outcome of theALL national protocols and explanations behind discrepancies in outcomes between institutions. Materials and
Methods: Between March 2006 and February 2008, 486 children with ALL from 12 institutions were enrolled inthe Thai national protocols. There were 3 different protocols based on specific criteria: one each for standard risk,high risk and Burkitt's ALL. We classified participating centers into 4 groups of institutions, namely: medicalschools in Bangkok, provincial medical schools, hospitals in Bangkok and provincial hospitals. We also evaluatedsupportive care, laboratory facilities in participating centers, socioeconomics, and patient compliance. Overalland event-free survival were determined for each group using the Kaplan Meier method. Statistical differenceswere determined using the log-rank test. Previous outcomes of Thai childhood ALL treatment between 2003 and2005 served as the historic control.
Results: Five-year overall survival of ALL treated using the Thai nationalprotocol was 67.2%; an improvement from the 63.7% of the 12-institute historical control (p-value=0.06). Therewere discrepancies in event-free survival of ALL between centers in Bangkok and up-country provinces (69.9%vs 51.2%, p-value <0.01). Socioeconomics and patient compliance were key elements in determining the outcome(65.5% vs 47.5%, 59.4% vs 42.9%) (p-value < 0.02).
Conclusions: Implementation of standard national protocolsfor childhood leukemia in Thailand did not significantly improve the outcome of ALL. Factors leading to betteroutcomes included (a) improvement of treatment compliance (b) prevention of treatment abandonment and (c)financial support to the family. Acute leukemia children national protocols Thailand 2015 11 01 4609 4614 http://journal.waocp.org/article_31133_f4c2314cb9ec1f983b0fa6bf74c94d51.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Perceptions of Turkish University Students about the Effects of Water Pipe Smoking on Health Background: The popularity of the water pipe, also referred to as hookah, narghile, shisha or hubble-bubble,has increased tremendously during the past few decades. This study was conducted to determine student waterpipe smoking status and perceptions about the effects of water pipe smoking on health in a state university inAnkara. Materials and
Methods: This cross-sectional study was conducted between September 2014 and January2015. The data were collected with a questionnaire and "The Scale of Perception about the Effects of Water PipeSmoking on Health". The data obtained were evaluated in IBM SPSS (version 20.0) statistical package programin computer. One-way analysis of variance (ANOVA) was used for the analyses by checking homogeneity ofvariances and Student's t-test. Values of p<0.05 were considered statistically significant.
Results: The total meanscore obtained by young people who took part in the study was determined as (X _=65.20±1.25, min=33, max=75).Upon comparison of the total mean scores obtained by young people from the Scale of Perception about theeffects of water pipe smoking on health and gender variable, the scores obtained by the females students werehigher than those of the male students with a statistically significant difference (t=7.525, p<0.05). A statisticallysignificant difference was observed between the total mean scores obtained by young people with cigarette andwater pipe smoking status (for each, t=-3.731, p<0.05; t=-13.987, p<0.05).
Conclusions: In conclusion, universitystudents have wrong knowledge on the dangers of water pipe smoking. There was a high prevalence of usingwater pipes among university students. Gender significantly affected the perceptions about the effect of waterpipe smoking on health in our sample. University Students water pipe smoking scale of perception about effects on health Turkey 2015 11 01 4615 4621 http://journal.waocp.org/article_31134_f398af1ec6f6d3d73692bc7518fa39a9.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Cancer/Testis OIP5 and TAF7L Genes are Up-Regulated in Breast Cancer Breast cancer still remains as the most frequent cancer with second mortality rate in women worldwide. Thereare no validated biomarkers for detection of the disease in early stages with effective power in diagnosis andtherapeutic approaches. Cancer/testis antigens are recently promising tumor antigens and suitable candidatesfor targeted therapies and generating cancer vaccines. We conducted the present study to analyze transcriptchanges of two cancer/testis antigens, OIP5 and TAF7L, in breast tumors and cell lines in comparison withnormal breast tissues by quantitative real time RT-PCR for the first time. Significant over-expression of OIP5was observed in breast tumors and three out of six cell lines including MDA-MB-468, T47D and SKBR3. Notsignificant expression of TAF7L was evident in breast tumors but significant increase was noted in three out ofsix cell lines including MDA-MB-231, BT474 and T47D. OIP5 has ssignificant role in chromatin organizationand cell cycle control during cell cycle exit and normal chromosome segregation during mitosis and TAF7L is acomponent of the transcription factor ІІD, which is involved in transcription initiation of most protein codinggenes. TAF7Lis located at X chromosome and belongs to the CT-X gene family of cancer/testis antigens whichcontains about 50% of CT antigens, including those which have been used in cancer immunotherapy. Breast cancer biomarkers targeted therapy cancer/testis genes OIP5 TAF7L 2015 11 01 4523 4528 http://journal.waocp.org/article_31135_f804f02664b3b2bb4a5af142d45c0375.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 A clinical Comparison of Lobaplatin or Cisplatin with Mitomycine and Vincristine in Treating Patients with Cervical Squamous Carcinoma Background: The research was to compare the efficacy and side effects of cisplatin or lobaplatin in combinationwith mitomycine (MMC) and vincristine in treating patients with cervical squamous carcinoma. Materials and
Methods: Cervical squamous carcinoma patients who were pathologically diagnosed with stage Ib-IIb from April2012 to May 2013 in the general hospital of Chinese People's Libration Amy were enrolled. All patients wereconfirmed without prior treatment and were randomly divided into two groups, Group A and B. Efficacy andside effects were evaluated after one cycle of chemotherapy.
Results: Group A (n=42) were treated with Loubo®(Lobaplatin) 50mg/m2, MMC 16mg/m2 and Vincristine 2mg/m2 every 21 days. Group B (n=44) were treatedwith Cisplatin 100mg/m2, MMC 16mg/m2 and Vincristine 2mg/m2 every 21 days. All 86 patients completed onecycle of chemotherapy with cisplatin or lobaplatin in combination with MMC and vincristine. No difference wasobserved regardiing short-term effect between two groups. Main side effects were bone marrow suppression andgastrointestinal reactions including decrease of white blood cells, platelet and nausea/vomiting. Grade III-Ⅵliver and kidney impairment was not reported in two groups. In group A the incidence of uterine artery spasmin the process of drug delivery was significantly lower than the group B.
Conclusions: Cisplatin or lobaplatinwith MMC and Vincristine in the interventional treatment of cervical squamous carcinoma were effective,especially after uterine artery perfusion chemotherapy at tumor reduction and tumor downstaging period. Theadverse reactions of concurrent chemotherapy are tolerable, and low physical and mental pressure even moreless stimulation of vascular in treatment with lobaplatin. However, the long-term effects of this treatment needfurther observation. Cisplatin lobaplatin side effect arterial perfusion 2015 11 01 4629 4631 http://journal.waocp.org/article_31136_b44dec6ae8cf2ca77ff32b4629fc0ea8.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Meta-Analysis of the Association between H63D and C282Y Polymorphisms in HFE and Cancer Risk Background: Previous studies suggested that the H63D and C282Y polymorphisms in the HFE genes weresusceptible to many cancer types, nevertheless, the present results were inconclusive. Thus, the present studywas aimed to evaluate the association between the HFE polymorphisms (H63D and C282Y) and cancer riskvia meta-analysis. Materials and
Methods: We retrieved PubMed, Google Scholar, Embase and Web of Sciencedatabases for all eligible studies up to April 1, 2015. All the statistical analysis was conducted by STATA 12.0.
Results: Finally, a total of 20 publications including 24 case-control studies, comprising 6,524 cases and 31,080controls for HFE-C282Y polymorphism and 19 publications including 21 case control studies, comprising 5,648cases and 14,257 controls for HFE-H63D polymorphism were enrolled in our analysis. An increased risk for overallcancer risk was identified in HFE-H63D polymorphism under allele contrast (D vs H: OR=1.153; 95%CI=1.031-1.289, Pheterogeneity=0.002), homozygotes vs wide type (DD vs HH: OR=1.449; 95%CI=1.182-1.777,Pheterogeneity=0.391), dominant model (DD+HD vs HH: OR=1.145; 95%CI=1.007-1.301, Pheterogeneity=0.002)and recessive model (DD vs HD+HH: OR=1.416 ; 95%CI=1.156-1.735, Pheterogeneity=0.549), as well as HFEC282Yunder homozygotes vs wide type (YY vs CC: OR=1.428, 95%CI=1.017-2.006, Pheterogeneity=0.220). Inaddition, in the stratified analysis by cancer type, an increased risk was identified in hepatocellular carcinomaand breast cancer in C282Y polymorphism, as well as pancreatic cancer in H63D polymorphism, whereas adecreased risk of colorectal cancer was identified in C282Y polymorphism.
Conclusions: Present study suggestedthat H63D and C282Y polymorphisms associated with an increased risk of overall cancer. Nevertheless, welldesignedstudy with large sample size will be continued on this issue of interest. HFE Polymorphism cancer Meta-analysis 2015 11 01 4633 4639 http://journal.waocp.org/article_31137_78014d530c1f5572bedc3c4fac333701.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethylacetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 humanlung adenocarcinoma epithelial cells. A-549 cells were exposed to 10-1000 μg/ml concentrations of the leaf extractsof A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyltetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced theviability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate,36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% withwater soluble extracts at 1000 μg/ml each. Among the various plant extracts, ethyl acetate extract showed thehighest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol,and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of differentsoluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of apotential therapeutic anticancer agents. Abelia triflora A-549 cell line Cytotoxicity MTT assay 2015 11 01 4641 4645 http://journal.waocp.org/article_31138_517df8f75aeee6e82d91a0eac117ce7a.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Epidemioclinical Feature of Early-Onset Colorectal Cancer at-Risk for Lynch Syndrome in Central Iran Background: Colorectal cancer (CRC) is becoming one of the most complicated challenges of human health,particularly in developing countries like Iran. In this paper, we try to characterize CRC cases diagnosed < age 50at-risk for Lynch syndrome within central Iran. Materials and
Methods: We designed a descriptive retrospectivestudy to screen all registered CRC patients within 2000-2013 in Poursina Hakim Research Center (PHRC), areferral gastroenterology clinic in central Iran, based on being early-onset (age at diagnosis ≤50 years) andAmsterdam II criteria. We calculated frequencies and percentages by SPSS 19 software to describe clinical andfamily history characteristics of patients with early-onset CRC.
Results: Overall 1,659 CRC patients were includedin our study of which 413 (24.9%) were ≤50 years at diagnosis. Of 219/413 successful calls 67 persons (30.6%)were reported deceased. Family history was positive for 72/219 probands (32.9%) and 53 families (24.2%) wereidentified as familial colorectal cancer (FCC), with a history of at-least three affected members with any type ofcancer in the family, of which 85% fulfilled the Amsterdam II Criteria as hereditary non-polyposis colorectalcancer (HNPCC) families (45/219 or 20.5%). Finally, 14 families were excluded due to proband tumor tissuesbeing unavailable or unwillingness for incorporation. The most common HNPCC-associated extracoloniccanceramong both males and females of the families was stomach, at respectively 31.8 and 32.7 percent. Themost common tumor locations among the 31 probands were rectum (32.3%), sigmoid (29.0%), and ascendingcolon (12.9%).
Conclusions: Given the high prevalence of FCC (~1/4 of early-onset Iranian CRC patients), it isnecessary to establish a comprehensive cancer genetic counseling and systematic screening program for earlydetection and to improve cancer prognosis among high risk families. colorectal cancer epidemioclinical feature Lynch syndrome Central Iran 2015 11 01 4647 4652 http://journal.waocp.org/article_31139_1a2abd3b39c8b6c74de1fbc0e73b0f1a.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Evaluation of Managerial Needs for Palliative Care Centers: Perspectives of Medical Directors Background: Palliative therapies have an important role in increasing the quality of healthcare and in dealingwith physical and psychosocial problems due to cancer. We here aimed to evaluate the managerial perspectivesand opinions of the hospital managers and clinical directors about specialized palliative care centers. Materialsand
Methods: This study was conducted in two large-scale hospitals in which oncology care is given with medicaldirectors (n:70). A questionnaire developed by the researchers asking about demographic characteristics andprofessional experience, opinions and suggestions of medical directors about providing and integrating palliativecare into healthcare was used and responses were analyzed.
Results: Potential barriers in providing palliativecare (PC) and integrating PC into health systems were perceived as institutional by most of the doctors (97%)and nurses (96%). Social barriers were reported by 54% of doctors and 82% of nurses. Barriers due to interestand knowledge of health professionals about PC were reported by 76% of doctors and 75% of nurses. Amongencouragement ideas to provide PC were dealing with staff educational needs (72%), improved working conditions(77%) and establishing a special PC unit (49)%. An independent PC unit was suggested by 27.7% of participantsand there was no difference between the hospitals. To overcome the barriers for integration of PC into healthsystems, providing education for health professionals and patient relatives, raising awareness in society, financialarrangements and providing infrastructure were suggested. The necessity for planning and programming wereemphasized.
Conclusions: In our study, the opinions and perspectives of hospital managers and clinical directorswere similar to current approaches. Managerial needs for treating cancer in efficient cancer centers, increasingthe capacity of health professionals to provide care in every stage of cancer, effective education planning andpatient care management were emphasized. palliative care Questionnaire delivery of healthcare patient care management 2015 11 01 4653 4658 http://journal.waocp.org/article_31140_15beab7a6d248f4371787683a63a33cb.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Quantitative Assessment of the Association between ABC Polymorphisms and Osteosarcoma Response: a Meta-analysis Background: ABC proteins are one key type of transport superfamilies which undertake majority of drugtransport, which affect the osteosarcoma response to chemotherapeutics. Previous studies have suggested theassociation between ABC polymorphisms and osteosarcoma response. However, the results of previous studiesremain controversial. Therefore, we perform a meta-analysis to get a more precise estimation of this association.The association between ABC polymorphisms and osteosarcoma response was assessed by odds ratios (ORs)together with their 95% confidence intervals (CIs). Three polymorphisms of ABC including ABCB1 rs1128503,ABCC3 rs4148416 and ABCC2 rs717620 polymorphism were investigated. Overall, significant association wasobserved between ABCC3 rs4148416 polymorphism and osteosarcoma response under allele contrast (T vs.C: OR=1.73, 95%CI=1.09-2.74, P=0.019), homozygote comparison (TT vs. CC: OR=2.00, 95%CI=1.25-3.23,P=0.004), recessive genetic model (TT vs. TC/CC: OR=1.80, 95%CI=1.14-2.84, P=0.011) and dominant geneticmodel (TT/TC vs. CC: OR=1.70, 95%CI=1.20-2.42, P=0.003). Moreover, significant association was also observedin Caucasian population rather than Asian population for ABCB1 rs1128503 polymorphism. We conclude thatABCC3 rs4148416 polymorphism was significantly associated with poor osteosarcoma response and ABCB1rs1128503 polymorphism was significantly associated with good osteosarcoma response in Caucasian populationrather than Asian population. ABC Polymorphism osteosarcoma tumor response Meta-analysis 2015 11 01 4659 4664 http://journal.waocp.org/article_31141_18833f723cfee961cca4cbad93f7725e.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Aberrant Methylation of RASSF1A gene Contribute to the Risk of Renal Cell Carcinoma: a Meta-Analysis The aim of this study was to assess the diagnostic value of RASSF1A methylation in renal cell carcinoma.Systematically search were performed using the Pubmed, ProQest and Web of Science for all articles on theassociation between RASSF1A methylation and renal cell carcinoma before 15 April 2015. After the filtration, 13studies involving 677 cases and 497 controls met our criteria. Our meta-analysis suggested that hypermethylationof RASSF1A gene was associated with the increased risk of RCC(OR:4.14, 95%CI:1.06-16.1). Stratified analysesshowed a similar risk in qualitative detection method(OR:28.4, 95%CI:10.2-79.6), body fluid sample(OR:12.8,95%CI:5.35-30.8), and American(OR:10.5, 95%CI:1.97-55.9). Our result identified that RASSF1A methylationhad a strong potential in prediction the risk of Renal cell carcinoma. RAS association domain family 1A renal cell carcinoma DNA Methylation Meta-analysis 2015 11 01 4665 4669 http://journal.waocp.org/article_31142_79a1583657de9404222ef90e0663212e.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuablenutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M.oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidantactivities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds.Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essentialoil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lineswere subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity wasassessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to theincreasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentrationapplied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2,where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore,the IC50 values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and 751.9 μg/mL, respectively.Conclusively, the present investigation provides preliminary results which suggest that seed essential oil fromM. oleifera has potent cytotoxic activities against cancer cell lines. anticancer agents Cytotoxicity medicinal plants Moringa seed oil 2015 11 01 4671 4675 http://journal.waocp.org/article_31143_91a79826d43d4a124ab855e87be55ec1.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Probability Sampling Method for a Hidden Population Using Respondent-Driven Sampling: Simulation for Cancer Survivors When there is no sampling frame within a certain group or the group is concerned that making its populationpublic would bring social stigma, we say the population is hidden. It is difficult to approach this kind ofpopulation survey-methodologically because the response rate is low and its members are not quite honest withtheir responses when probability sampling is used. The only alternative known to address the problems causedby previous methods such as snowball sampling is respondent-driven sampling (RDS), which was developed byHeckathorn and his colleagues. RDS is based on a Markov chain, and uses the social network information ofthe respondent. This characteristic allows for probability sampling when we survey a hidden population. Weverified through computer simulation whether RDS can be used on a hidden population of cancer survivors.According to the simulation results of this thesis, the chain-referral sampling of RDS tends to minimize as thesample gets bigger, and it becomes stabilized as the wave progresses. Therefore, it shows that the final sampleinformation can be completely independent from the initial seeds if a certain level of sample size is secured evenif the initial seeds were selected through convenient sampling. Thus, RDS can be considered as an alternativewhich can improve upon both key informant sampling and ethnographic surveys, and it needs to be utilized forvarious cases domestically as well. Respondent-driven sampling cancer survivors social network hidden population Simulation 2015 11 01 4677 4683 http://journal.waocp.org/article_31144_78c84df7a661b5bdf8095c10a8511fa2.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Cosmetic Outcomes and Quality of Life in Thai Women Post Breast Conserving Therapy for Breast Cancer Purpose: To evaluate the correlation between cosmetic outcome (CO), body image, and quality of life in postbreast-conserving therapy (BCT) women. Materials and
Methods: This cross-sectional study concerned one-yearpost-completed BCT Thai women. The data included subjective and objective CO with a questionnaire coveringdemographic and clinical data, anti-hormonal treatment status, Eastern Cooperative Oncology Group (ECOG)performance status, Self-Reported Cosmetic Outcomes (SRCO), Self-Reported Breast Symmetry (SRBS), BodyImage Scale (BIS), and the Functional Assessment of Cancer Therapy with Breast Cancer subscale (FACT-B).Participants had breast photographs taken for the evaluation of objective cosmetic outcome (OCO) after breastcancer conservation treatment. The relationship between CO and FACT-B was tested using Spearman's rankcorrelation
Results: A total 127 participants volunteered for the study. The participant characteristics were age52(±9), Buddhist 87%, married 65%, body mass index 25.0(±4.6), breast cup size A-C 91%, college educated60%, employed 66%, ECOG 0-1 95%, tumor size less than or equal to 2 cm 55%, no lymph node metastasis98%, and taking tamoxifen 57%. Two percent of the participants regretted their decision to undergo BCT. TheSRCO was excellent in 2%, good in 68%, fair in 30%, and poor in 0%. For SRBS, rates were 17%, 58%, 24%and 1% for excellent, good, fair and poor cosmetic outcomes, respectively. The BCCT scores were excellent 24%,good 39%, fair 32%, and poor 6%. The median total QOL score of the participants was 130 (93-144). There wasno significant correlation between CO and FACT-B scores.
Conclusions: The significance of CO for FACT-B inThai women with breast cancer could not be assessed in detail because of a very low level of correlation. Theresults may be due to the effects of cultural background. breast cancer cosmetic outcome breast-conserving therapy Quality of Life 2015 11 01 4685 4690 http://journal.waocp.org/article_31145_0843c0c6f227fc778636905f2780228e.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Loss of Expression of PTEN is Associated with Worse Prognosis in Patients with Cancer Background: The tumor suppressor phosphatase and tensin homolog (PTEN) is an important negativeregulator of cell-survival signaling. However, available results for the prognostic value of PTEN expression inpatients with cancer remain controversial. Therefore, a meta-analysis of published studies investigating thisissue was performed. Materials and
Methods: A literature search via PubMed and EMBASE databases wasconducted. Statistical analysis was performed by using the STATA 12.0 (STATA Corp., College, TX). Data fromeligible studies were extracted and included into the meta-analysis using a random effects model.
Results: A totalof 3,810 patients from 27 studies were included in the meta-analysis, 22 investigating the relationship betweenPTEN expression and overall survival (OS) using univariate analysis, and nine with multivariate analysis. Thepooled hazard ratio (HR) for OS was 1.64 (95% confidence interval (CI): 1.32-2.05) by univariate analysis and1.56 (95% CI: 1.20-2.03) by multivariate analysis. In addition, eight papers including two disease-free-survivalanalyses (DFSs), four relapse-free-survival analyses (RFSs), three progression-free-survival analyses (PFSs) andone metastasis-free-survival analysis (MFS) reported the effect of PTEN on survival. The results showed thatloss of PTEN expression was significant correlated with poor prognosis, with a combined HR of 1.74 (95% CI:1.24-2.44). Furthermore, in the stratified analysis by the year of publication, ethnicity, cancer type, method, cutoffvalue, median follow-up time and neoadjuvant therapy in which the study was conducted, we found that theethnicity, cancer type, method, median follow-up time and neoadjuvant therapy are associated with prognosis.
Conclusions: Our study shows that negative or loss of expression of PTEN is associated with worse prognosis inpatients with cancer. However, adequately designed prospective studies need to be performed for confirmation. cancer PTEN Overall survival Meta-analysis 2015 11 01 4691 4698 http://journal.waocp.org/article_31146_296a92ea4b2b36a301a417a597a8653c.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Nested Case-control Study of Occupational Radiation Exposure and Breast and Esophagus Cancer Risk among Medical Diagnostic X Ray Workers in Jiangsu of China Medical diagnostic X-ray workers are one occupational group that expose to the long-term low-dose externalradiation over their working lifetime, and they may under risk of different cancers. This study aims to determinethe relationship between the occupational X-ray radiation exposure and cancer risk among these workers inJiangsu, China. We conducted Nested case-control study to investigate the occupational X-ray radiation exposureand cancer risk. Data were collected through self-administered questionnaire, which includes but not limitsto demographic data, personal behaviors and family history of cancer. Retrospective dose reconstruction wasconducted to estimate the cumulative doses of the x-ray workers. Inferential statistics, t-test and 2 tests wereused to compare the differences between each group. We used the logistic regression model to calculate the oddsratio (OR) and 95% confidence interval (CI) of cancer by adjusting the age, gender. All 34 breast cancer casesand 45 esophageal cancer cases that detected in a cohort conducted among health workers between 1950~2011were included in this presented study, and 158 cancer-free controls were selected by frequency-matched (1:2).Our study found that the occupational radiation exposure was associated with a significantly increased cancerrisk compared with the control, especially in breast cancer and esophageal cancer (adjusted OR=2.90, 95% CI:1.19-7.04 for breast cancer; OR=4.19, 95% CI: 1.87-9.38 for esophageal cancer, and OR=3.43, 95% CI: 1.92-6.12for total cancer, respectively). The occupational X-ray radiation exposure was associated with increasing cancerrisk, which indicates that proper intervention and prevention strategies may be needed in order to bring downthe occupational cancer risk. nested case-control study occupational radiation medical diagnostic X ray workers Cancer risk 2015 11 01 4699 4704 http://journal.waocp.org/article_31147_01388a27cb544d5ddd6bb3a83678c4b9.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Short Sleep Duration and Its Correlates among Cancer Survivors in Korea: the Korea National Health and Nutrition Examination Surveys Background: Though a large proportion of cancer survivors are assumed to be commonly affected by sleepdisturbance, few studies have focused on short sleep problems and its correlates among Korean cancer survivors.The purpose of this study was to evaluate the prevalence of short sleep in adult cancer survivors from a nationwidepopulation-based sample and to identify risk factors for short sleep duration. Materials and
Methods: Based onthe fourth and fifth Korea National Health and Nutrition Examination Surveys (2007-2012), 1,045 cancer survivorsand 33,929 non-cancer controls were analyzed. The prevalence of short sleep was compared between these twogroups. Associations between short sleep and its correlates were evaluated using multiple logistic regression amongcancer survivors: odds ratios (ORs) and 95% confidence intervals (95%CIs) were estimated after adjusting forsociodemographic factors, lifestyle factors, psychological conditions, and cancer-related factors.
Results: About8.1% of cancer survivors slept for less than 5 hours per day (6.2% men and 9.3% women), whereas this was thecase for only 3.7% of non-cancer controls. Cancer survivors who had the lowest household income level showeda significantly higher likelihood for short sleep (adjusted OR 2.82, 95%CI 1.06-7.54). Self-reported poor healthand depressive symptoms were found to be associated with significantly increased likelihood for short sleep incancer survivors (adjusted OR 3.60, 95%CI 1.40-9.26 and adjusted OR 2.00, 95%CI 1.17-3.42). Gastric cancersurvivors had a 3.97-fold increased risk for short sleep (95%CI 1.60-9.90).
Conclusions: The prevalence of shortsleep occurs at a high rate among the Korean cancer survivors, which may indicate a poorer quality of life anda higher risk of future complications in survivorship. Targeted interventions that can assist cancer survivorsto cope with sleep disturbances as well as ensuring psychological stability are warranted to reduce the latentdisease burden. Cancer survivorship sleep disturbance risk factors Korea 2015 11 01 4705 4710 http://journal.waocp.org/article_31148_fb52abbc6dcea12b1c7156ec03b5ac0b.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Radical Oncological Surgery and Adjuvan Therapy in Non-Small Cell Lung Cancer Patients over 70 years of Age Background: The incidence of lung cancer increases with age. Approximately 50% of non-small cell lung cancer(NSCLC) patients are over 70 years old. Because of the increasing elderly population, treatment approaches inthis age group continue to be studied similar to groups of young people. Materials and
Methods: In the currentstudy, 26 patients who underwent radical surgery and adjuvan chemoradiation at Ataturk Chest Diseases andChest Surgery Training and Research Hospital were evaluated retrospectively.
Results: Of 21 patients (81%)were male and the average age was 74.4. Lobectomy was performed in 18 cases, pneumonectomy in 3, sleevelobectomy in 3 and bilobectomy in 2. There was no perioperative or early period mortality. Overall survival was24.5 months.
Conclusions: From our study, lung cancer surgery and adjuvant therapy can be performed safelywith low morbidity in the elderly. Elderly Lung cancer radical surgery Chemotherapy radiotherapy 2015 11 01 4711 4714 http://journal.waocp.org/article_31149_dbbf8c6f404b5d9fc42640ae47f55ef5.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 A Pilot Study on Factors Associated with Presentation Delay in Patients Affected with Head and Neck Cancers Background: Patient delay can contribute to a poor outcome in the management of head and neck cancers(HNC). The main objective of the present study was to investigate the factors associated with patient delay inour population. Materials and
Methods: Patients with cancers of the head and neck attending a regional cancercenter of North East India were consecutively interviewed during the period from June 2014 to November 2014.The participation of patients was voluntary. The questionnaire included information on age, gender, residentialstatus, educational qualification, monthly family income, any family history of cancer, and history of priorawareness on cancer from television (TV) program and awareness program.
Results: Of 311 (n) patients, withan age range of 14-88 years (mean 55.4 years), 81.7% were males and 18.3% females (M:F=4.4). The overallmedian delay was 90 days (range=7 days-365 days), in illiterate patients the median delay was 90 days and 60days in literate patients (P=0.002), the median delay in patients who had watched cancer awareness program onTV was 60 days and in patients who were unaware about cancer information from TV program had a mediandelay of 90 days (p=0.00021) and delay of <10 weeks was seen in 139 (44.6%) patients, a delay of 10-20 weeks in98 (31.5%) patients, and a delay of 20-30 weeks in 63 (20.2%) patients.
Conclusions: Education and awarenesshad a significant impact in reduction of median patient delay in our HNC cases. delay head and neck cancer Health Education median patient delay 2015 11 01 4715 4718 http://journal.waocp.org/article_31150_f52be817f0102a58dedbb0e797a6ff40.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 The Level of Squamous Cell Carcinoma Antigen and Lymph Node Metastasis in Locally Advanced Cervical Cancer Background: This study aimed to determine the utility and a cut-off level of serum squamous cell carcinomaantigen (SCC-Ag) to predict lymph node metastasis in locally advanced cervical cancer cases. We also investigatedthe correlation between SCC-Ag level and lymph node status. Materials and
Methods: From June 2009 to June2014, 232 patients with cervical cancer stage IB2-IVA, who were treated at Ramathibodi Hospital, were recruited.Receiver operating characteristic (ROC) curves were used to identify the best cut-off point of SCC-Ag level topredict lymph node metastasis. Quantile regression was performed to evaluate the correlation between SCC-Aglevels and pelvic lymph node metastasis, paraaortic lymph node metastasis, and parametrial involvement as wellas tumor size.
Results: Pelvic lymph node metastasis and paraaortic lymph node metastasis were diagnosed in46.6% and 20.1% of the patients, respectively. The median SCC-Ag level was 6 ng/mL (range, 0.5 to 464.6 ng/mL). The areas under ROC curves between SCC-Ag level and pelvic lymph node metastasis, paraaotic lymphnode metastasis, parametrial involvements were low. SCC-Ag level was significantly correlated with paraaorticlymph node status (p=0.045) but not with pelvic lymph node status and parametrial involvement. SCC-Ag levelwas also related to the tumor diameter (p<0.05).
Conclusions: SCC-Ag level is not a good predictor for pelvicand paraaortic lymph node metastasis. However, it is still beneficial to assess the tumor burden of squamouscell carcinoma of the cervix. cervical cancer squamous cell carcinoma antigen Lymph node metastasis tumor size 2015 11 01 4719 4722 http://journal.waocp.org/article_31151_4de52f8a26f03287be4dd68d3e7ee175.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Radiotherapy and Concurrent Chemo-Radiotherapy in Locally Advanced Hypopharyngeal Cancers - A Hospital Registry Based Analysis Background: The survival of patients with hypopharyngeal cancer is low amongst head and neck cancer cases.The incidence rates of hypopharyngeal cancers in our population are amongst the highest in the world and thereare limited data available on the literature on varied responses to first course of treatment with radiotherapy (RT)and concurrent chemo-radiotherapy (CRT) in our population. Materials and
Methods: Clinical characteristicsand initial responses to treatment in patients who had received radiotherapy and chemo-radiotherapy in aregional cancer center from January 2010 to December 2013 were evaluated. The data were obtained from thehospital cancer registry, and analysis was carried using descriptive statistics. Pearson's chi-square was usedto test for differences in the variables and p<0.05 was considered statistically significant.
Results: A total of554 patients were included in the analysis, 411 (74.2%) receiving RT and 143 (25.8%) being given CRT. Therewas significantly lower number of patients above 70 years with a higher proportion of patients below 50 yearswho had received CRT (p<0.05). Some 79.3% and 84.6% of patients in the RT and CRT groups respectivelypresented with a favorable performance status, and in the RT group 240 (58.4%) showed complete response(CR), and in the CRT group 103 (72.0%) showed CR at the first follow-up (p<0.05).
Conclusions: Concurrentchemo-radiotherapy gives better short term response to treatment in locally advanced hypopharyngeal cancers. Hypopharyngeal cancers radiotherapy chemo-radiotherapy responses to treatment 2015 11 01 4723 4726 http://journal.waocp.org/article_31152_7a52326624730ab1f2ee40cc27489120.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Mutation Screening and Association Study of the Folylpolyglutamate Synthetase (FPGS) Gene with Susceptibility to Childhood Acute Lymphoblastic Leukemia Background: Folylpolyglutamate synthetase (FPGS), an important enzyme in the folate metabolic pathway,plays a central role in intracellular accumulation of folate and antifolate in several mammalian cell types. Loss ofFPGS activity results in decreased cellular levels of antifolates and consequently to polyglutamatable antifolatesin acute lymphoblastic leukemia (ALL). Materials and
Methods: During May 1997 and December 2003, 134children diagnosed with ALL were recruited from one hospital in Thailand. We performed a mutation analysis inthe coding regions of the FPGS gene and the association between single nucleotide polymorphisms (SNPs) withinFPGS in a case-control sample of childhood ALL patients. Mutation screening was conducted by polymerasechain reaction-single strand conformation polymorphism (PCR-SSCP) and subsequently with direct sequencing(n=72). Association analysis between common FPGS variants and ALL risk was done in 98 childhood ALL casesand 95 healthy volunteers recruited as controls.
Results: Seven SNPs in the FPGS coding region were identifiedby mutation analysis, 3 of which (IVS13+55C>T, g.1297T>G, and g.1508C>T) were recognized as novel SNPs.Association analysis revealed 3 of 6 SNPs to confer significant increase in ALL risk these being rs7039798 (p=0.014, OR=2.14), rs1544105 (p=0.010, OR= 2.24), and rs10106 (p=0.026, OR= 1.99).
Conclusions: These findingssuggested that common genetic polymorphisms in the FPGS coding region including rs7039789, rs1544105, andrs10106 are significantly associated with increased ALL risk in Thai children. Folylpolyglutamate synthetase Acute Lymphoblastic Leukemia Single nucleotide polymorphism 2015 11 01 4727 4732 http://journal.waocp.org/article_31153_091cc903cbe90ccdedf2717275ff4e60.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Quantitative Review of Oral Cancer Research Output from Pakistan Background: Oral cancer is the most common cancer among men and second only to breast cancer amongwomen in Pakistan. For the effective control and prevention of oral cancer, Pakistan needs to recognize theimportance of research and generation of the evidence-base which can inform policy making and planning andimplementation of intervention programs. The objective of this review was to quantify oral cancer researchoutput in Pakistan. Materials and
Methods: A systematic electronic search in "Medline", "ISI-Web of Science"and "Pakmedinet", supplemented by a Google search, was carried out in January and February, 2014, to identifyliterature from Pakistan relevant to oral cancer. The selection of publications for the review was carried outaccording to preset criteria. Data were recorded and analyzed using Microsoft Excel.
Results: A total of 166publications comprising 62 case series, 36 cross sectional, 31 case control, 10 basic laboratory research, elevenreviews and two trials, were included in this review. Some 35 % of the publications focused on risk factors fororal cancer. COMSATS Institute of Information Technology was the institution with the highest contribution.
Conclusions: There is a lack of research in the field of oral cancer research in Pakistan. Focused efforts shouldbe put in place to improve both quality and quantity of oral cancer research in the country. oral cancer Research Pakistan 2015 11 01 4733 4739 http://journal.waocp.org/article_31154_e97bd9a7e380ca5d7e2683d8c36bf0e7.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Knowledge, Attitudes and Practice of Testicular Selfexamination among Male University Students from Bangladesh, Madagascar, Singapore, South Africa and Turkey The aim of this study was to investigate the knowledge, attitude and practice of testicular self-examination(TSE) among male university students from low income (Bangladesh, Madagascar), middle income (South Africa,Turkey) and emerging economy (Singapore) countries. Using anonymous questionnaires, data were collectedfrom 2,061 male undergraduate university students aged 16-30 (mean age 21.4, SD=2.4) from 5 universities in 5countries across Asia and Africa. Overall, 17.6% of the male students indicated that they knew how to performTSE; this knowledge proportion was above 20% in Bangladesh and Singapore, while it was the lowest (12.2%)in Madagascar. Among all men, 86.4% had never practiced TSE in the past 12 months, 7.1% 1-2 times, 3.5%3-10 times, and monthly TSE was 3.1%. The proportion of past 12 month TSE was the highest (17.6%) amongmale university students in South Africa and the lowest (7.3%) among students in Singapore. Logistic regressionfound that TSE importance or positive attitude was highly associated with TSE practice. TSE practices werefound to be inadequate and efforts should be made to develop programmes that can increase knowledge relatedto testicular cancer as well as the practice of testicular self-examination. Testicular self-examination Testicular cancer Knowledge Attitude Practice University Students multi 2015 11 01 4741 4743 http://journal.waocp.org/article_31155_96ca896a21830a0edeb90a681803cc43.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Secondary Circulating Tumor Cells (CTCs) but not Primary CTCs are Associated with the Clinico-Pathological Parameters in Chilean Patients With Colo-Rectal Cancer Background: The aim of this study was to assess detection of circulating tumor cells (CTC) using anti-CEApre and post surgery in Chilean patients with colo-rectal cancer. Materials and
Methods: The presence of CTCswas evaluated in 80 colorectal cancer patients pre and post surgery using standard immunocytochemistry andthe results were compared with findings for standard clinico-pathological parameters.
Results: In patients presurgeryCEA (+) CTCs were frequently found, with no relation to tumor size or nodal status. After surgery, thepresence of CTCs was associated with such clinico-pathological parameters. The frequency of CTC detection innode positive patients did not change after surgery. In patients with metastasis there was also no change in thefrequency of CTC detection, and clusters of 3 or more CTCs were evident.
Conclusions: Secondary CTCs areassociated with clinico-pathological parameters only after surgical removal of the primary tumor, and mightbe important in identifying patients at high risk of relapse. Primary CTCs detected before surgical removal arefrequently found, are not associated with the clinico-pathological parameters and might have a role in cancerscreening. These findings suggest the need for studies with a larger population of patients. colorectal cancer circulating tumor cells CEA 2015 11 01 4745 4749 http://journal.waocp.org/article_31156_e0f0b56e3aea97fdaa4080aae1234f91.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus) Echinodermata use saponins in chemical defense against pathogens and predators. The molecular mechanismsof antimetastatic effects of brittle star saponins are still unknown. The present study examined antioxidantcapacity and invasive ability in HeLa carcinoma cells exposed to brittle star crude saponins. Discolorating methodswith DPPH and ABTS and expression of SOD-2 with RT-PCR were used to estimate the antioxidant activity.The anti-invasive activity of extracted saponins was examined through adhesion of HeLa cells to extracellularmatrix, wound healing and evaluation of the mRNA levels of MMP-2 and MMP-9 by real time-PCR. The resultsshowed that extracted saponins had cytotoxicity against cervical cancer cells and ABTS and DPPH scavengingproperties with IC50 values of 604.5, 1012 μg/ml, respectively. Further, we found that, in wound healing assay,brittle star saponins could prevent invasion of HeLa cells in a concentration dependent manner. Furthermore, celladhesion assay demonstrated blockage of cell attachment to extracellular matrix with an IC50 concentration of16.1μg/ml. The significant dose dependent down regulation of MMP-2 and MMP-9 in treated cells demonstratedthat isolated saponins can decline tumor metastasis in vitro. The brittle star saponins remarkably preventedcervical cancer invasion and migration associated with down regulation of matrix metalloproteinase expression.Therefore, saponins could be suggested as an anti-invasive candidate against cervical cancer and an antioxidantas well. Echinoderm saponins cervical cancer Attachment aggression Antioxidant Capacity 2015 11 01 4751 4758 http://journal.waocp.org/article_31157_49994600c49503d125d0978f788c31c2.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Predictive and Prognostic Biomarkers for Patients Treated with Anti-EGFR Agents in Lung Cancer: A Systemic Review and Meta-Analysis Background: Several studies have investigated predictive and prognostic biomarkers for patients treated withanti-epidermal growth factor receptor (EGFR) agents in lung cancer. However, the conclusion is controversial.Materials and
Methods: A meta-analysis was conducted to evaluate the associations of mutant K-ras, PIK3CAand PTEN deficiency with the efficacy of anti-EGFR agents in lung cancer. The primary endpoint was objectiveresponse rate (ORR). The secondary endpoints were overall survival (OS) and progression-free survival (PFS).
Results: A total of 61 studies were included in the final meta-analysis. The result showed that K-ras mutationwas a good predictor for ORR (RR=0.42, 95%CI, 0.33-0.55, p=0.000) and an effective prognostic marker for OS(HR=1.37, 95%CI, 1.15-1.65, p=0.001) and PFS (HR=1.33, 95%CI, 1.05-1.69, p=0.019). However, PTEN deficiencyor PIK3CA mutation did not show any significance predictive value for ORR (PTEN, RR=0.82, 95%CI, 0.56-1.19,p=0.286; PIK3CA, RR=1.08, 95%CI, 0.17-6.66, P=0.938). And PTEN deficiency or expression of PIK3CA didnot show significance prognostic value for OS (PTEN, HR=0.88, 95%CI, 0.31-2.46,P=0.805; PIK3CA, HR=0.79,95%CI: 0.23-2.68, P=0.706).
Conclusions: Our meta-analysis showed that K-ras mutation may be an effectivepredictor in lung cancer patients treated with anti-EGFR agents. Whereas, the predictive and prognostic valueof PTEN deficiency and PIK3CA mutation need to be further investigated. Hung cancer K-ras mutation loss of PTEN PIK3CA mutation anti-EGFR agents 2015 11 01 4759 4768 http://journal.waocp.org/article_31158_233fb3ab2517ee0a76e800e41bdc4a85.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Roles of Combined Glypican-3 and Glutamine Synthetase in Differential Diagnosis of Hepatocellular Lesions Background: Hepatocellular carcinoma (HCC) is the fifth most prevalent cancer and thirdly leading causeof cancer-related death worldwide. The estimated risk of hepatocellular carcinoma is 15 to 20 times as highamong persons infected with HCV as it is among those who are not infected, with most of the excess risk limitedto those with advanced hepatic fibrosis or cirrhosis. Glypican3 (GPC3) plays a key role in relation to signalingwith growth factors, regulating the proliferative activity of cancer cells. Glutamine synthetase (GS) catalyzesthe synthesis of glutamine from glutamate and ammonia in the mammalian liver. GS was suggested as a specificmarker for tracing cell lineage relationships during hepatocarcinogenesis. In normal liver, GS expression isseen in pericentral hepatocytes, but not by midzonal or periportal hepatocytes. In HCC, strong and diffuseGS expression in seen in tumor cells.
Results: Glypican3 immunopositvity was highly specific and sensitiveindicator for hepatocellular carcinoma as well as glutamine synthetase which was found to be a sensitive andspecific indicator for development of hepatocellular carcinoma when compared to cirrhosis, liver cell dyspalsiaand metastatic carcinomas. Statistical analysis revealed a significant association between GPC3 and GS withtumor size (P=0.003, p=0.006, respectively). Diffuse staining significantly associated with large tumor size while,focal and mixed staining was detected more with small tumor size. Studying the relation with tumor grade alsorevealed significant association between diffuse GPC3 and GS staining with high tumor grade. Diffuse stainingwas detected in 91.7% and 100% respectively of poorly differentiated specimens and only in 33.3% and 22.2%of well differentiated specimens.
Conclusions: While using GPC3 and GS to screen for premalignant hepaticlesions remains controversial, our data suggest that GPC3 and GS may be a reliable diagnostic immunomarkersto distinguish HCC from benign hepatocellular lesions. However, negative immunostaining should not excludethe diagnosis of HCC. Glypican3 glutamine synthetase Hepatocellular carcinoma 2015 11 01 4769 4775 http://journal.waocp.org/article_31159_028768b0634b6567c423cd1bf2d259c4.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Adult Urological Soft Tissue Sarcomas: A Multicenter Study of the Anatolian Society of Medical Oncology (ASMO)
Objective: To analyze clinicopathological characteristics, prognostic factors and survival rates of thepatients with urological soft tissue sarcomas treated and followed up in Turkey. Materials and
Methods: Foroverall survival analyses the Kaplan-Meier method was used. From medical records, nine prognostic factors onoverall survival were analysed.
Results: For the 53 patients (34 males, 19 females) whose charts were reviewed,the median age was 53 (range 22 to 83) years. Most frequently renal location (n=30; 56.6%) was evident andleiomyosarcoma (n=20, 37.7%) was the most frequently encountered histological type. Median survival timeof all patients was 40.3 (95% CI, 14.2-66.3) months. In univariate analysis, male gender, advanced age (≥50years), metastatic stage, unresectability, grade 3, renal location were determined as worse prognostic factors.In multivariate analysis, metastatic stage, unresectability and grade 3 were determined as indicators of worseprognosis.
Conclusions: Urological soft tissue sarcomas are rarely seen tumours in adults. The most importantfactors in survival are surgical resection, stage of the tumour at onset, grade and location of the tumour, genderand age of the patients. Urologic sarcoma survival Prognostic factors Turkey 2015 11 01 4777 4780 http://journal.waocp.org/article_31160_39ba1e849698b5a46766209fbe514e34.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Proliferative and Inhibitory Activity of Siberian ginseng (Eleutherococcus senticosus) Extract on Cancer Cell Lines; A-549, XWLC-05, HCT-116, CNE and Beas-2b Siberian ginseng (Eleutherococcus senticosus) is used primarily as an adaptogen herb and also for its immunestimulant properties in Western herbal medicine. Another closely related species used in East Asian medicinesystems i.e. Kampo, TCM (Manchuria, Korea, Japan and Ainu of Hokkaido) and also called Siberian ginseng(Acanthopanax senticosus) also displays immune-stimulant and anti-cancer properties. These may affect tumourgrowth and also provide an anti-fatigue effect for cancer patients, in particular for those suffering from lungcancer. There is some evidence that a carbohydrate in Siberian ginseng may possess not only immune stimulatorybut also anti-tumour effects and also display other various anti-cancer properties. Our study aimed to determinethe inhibitory and also proliferative effects of a methanol plant extract of Siberan ginseng (E. senticosus) onvarious cancer and normal cell lines including: A-549 (small cell lung cancer), XWLC-05 (Yunnan lung cancercell line), CNE (human nasopharyngeal carcinoma cell line), HCT-116 (human colon cancer) and Beas-2b (humanlung epithelial). These cell lines were treated with an extract from E. senticosus that was evaporated and reconstitutedin DMSO. Treatment of A-549 (small cell lung cancer) cells with E. senticosus methanolic extractshowed a concentration-dependent inhibitory trend from 12.5 - 50μg/mL, and then a plateau, whereas at 12.5and 25 μg/mL, there is a slight growth suppression in QBC-939 cells, but then a steady suppression from 50, 100and 200μg/mL. Further, in XWLC-05 (Yunnan lung cancer cell line), E. senticosus methanolic extract displayedan inhibitory effect which plateaued with increasing dosage. Next, in CNE (human nasopharyngeal carcinomacell line) there was a dose dependent proliferative response, whereas in Beas-2 (human lung epithelial cell line),an inhibitory effect. Finally in colon cancer cell line (HCT-116) we observed an initially weak inhibitory effectand then plateau. Eleutherococcus senticosus A-549 (small cell lung cancer) XWLC-05 (Yunnan lung cancer cell line) 2015 11 01 4781 4786 http://journal.waocp.org/article_31161_a02bdd20336d9210237e3594ff7fdc60.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Ovarian Cancer in Children and Adolescents: Treatment and Reproductive Outcomes
Objective: To review ovarian cancer cases in children and adolescents in Siriraj Hospital and assessthe prognosis, recurrence of disease, and reproductive outcomes after treatment. Materials and
Methods:A retrospective descriptive study was conducted in ovarian cancer patients 21 years and younger who hadbeen treated at Siriraj Hospital between January 1990 and December 2009. Medical records were reviewedand relevant data were recorded.
Results: A total of 48 cases met the criteria; their mean age was 16.4 years.Abdominal distension was the major symptom. 91.6% were germ cell tumors and the remaining cases weresex cord-stromal and epithelial tumors. More than half (25/48 cases) presented with stage I disease. The mostcommon used chemotherapy regimen for germ cell tumors was BEP (bloemycin, etoposide, cisplatin). Most ofpatients had favorable outcomes; 46/48 cases had complete remission and retained their good health at the timeof the review. We had only one recurrent case and one dead case. Ten of contacted patients had married and 3of them had successful full-term pregnancies.
Conclusions: Ovarian malignancy in children and adolescents isa rare disease. The authors reported 48 cases in 20 year-period of work. Most of them have favorable outcomes.Return of ovarian function and fertility are the topics of interest. Adolescent children Ovarian Cancer treatment Reproduction 2015 11 01 4787 4790 http://journal.waocp.org/article_31162_a0165c5ae8430f1dcff2fc6e2dbc5c6e.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Study of Pemetrexed-based Chemotherapy for Patients with Locally Advanced or Metastatic Cancers Purpose: This study was conducted to observe the efficacy and safety of pemetrexed based chemotherapyin treating patients with locally advanced or metastatic cancers as first-line, second-line or third-line therapy.Materials and
Methods: From May 2011 to January 2015, we recruited 29 patients with advanced breast cancer,19 patients with advanced ovary cancer, 17 patients with advanced esophageal cancer,5 patients with advancedgallbladder cancer,5 patients with advanced cervical cancer and 1 patient with advanced tongue cancer inJiangsu Cancer Hospital and Research Institute.All of them were pathologically confirmed and treated withpemetrexed based chemotherapy. After two cycles of treatment,efficacy and safety can be evaluated.
Results:For pemetrexed based regimens,including 76 patients with 6 kinds of advanced cancer were considered eligiblefor inclusion. Complete remission represents CR, partial remission represents PR, stable disease represents SD,progressive disease represents PD. Among 29 patients with advanced breast cancer, 4 patients chose pemetrexedbased regimens as second-line treatment,1 of them was PR,the other 3 got SD. The last 25 patients made useof this chemotherapy as third-line treatment, except one patient could not be assessed, 2 of them got PR,6 ofthem got SD,the remaining 16 of them finally were PD.19 patients with advanced ovary cancer,5 patients usedthis regimens as second-line treatment, 3 of them got PD,the remaining patients got SD, respectively. The last14 patients made use of pemetrexed based regimens as third-line treatment,. RR (CR+PR) was 28.5%. Among17 patients with advanced esophageal cancer, 2 patients made use of pemetrexed based regimens as first-linetreatment,both of them got PR.4 of them used this chemotherapy as second-line regimen, except 2 patients couldnot be assessed,the remaining 2 was PD at last. The last 11 patients was third-line users, RR (CR+PR) was 18.2%.Among 5 patients with advanced gallbladder cancer, pemetrexed based regimens was used in 1 patient as firstlinetreatment and 1 patient as second-line treatment. The curative effect was SD and PD, respectively. 3 patientsaccepted pemetrexed based regimens as third-line treatment, 2 of them got PD as results and another was SD.Among 5 patients with advanced cervical cancer, just 1 patient adopted pemetrexed based regimens as first-linetreatment, whose curative effect was PR.2 patients chose this chemotherapy regimens as second-line treatment.Both of them got PD as their consequence. The last 2 patients made use of the regimens as third-line treatment,the effect of them was PD and SD, respectively. The one who with advanced tongue cancer, pemetrexed basedregimens was used as second-line treatment, and the consequence was PD. About 71.1% patients experienced bonemarrow suppression. Among them, 5 patients reached 4 grade. Other toxicity of pemetrexed were neurotoxicity,fatigue, diarrhea, dysphagia and vomiting. No treatment related death occurred with pemetrexed-based treatment.
Conclusions: Pemetrexed based chemotherapy has considerable effect in patients with advanced cancers suchas breast cancer,esophageal cancer and ovary cancer. More randomly clinical trials are needed to verify theresults. Based chemotherapy advanced or matastatic cancer efficacy and safety 2015 11 01 4791 4795 http://journal.waocp.org/article_31163_670cbfa36e9bc904b13c3e452e088cbf.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 A Pooled Analysis on Crizotinib in Treating Chinese Patients with EML4-ALK Positive Non-small-cell Lung Cancer Background: This analysis was conducted to evaluate the efficacy and safety of crizotinib based regimens intreating Chinese patients with EML4-ALK positive non-small-cell lung cancer. Materials and
Methods: Clinicalstudies evaluating the efficacy and safety of crizotinib based regimens on response and safety for Chinese patientswith EML4-ALK positive non-small-cell lung cancer were identified by using a predefined search strategy.Pooled response rate (RR) of treatment were calculated.
Results: In crizotinib based regimens, 3 clinical studieswhich including 128 Chinese patients with EML4-ALK positive non-small-cell lung cancer and treated withcrizotinib based regimen were considered eligible for inclusion. Pooled analysis suggested that, in all patients,the pooled RR was 59.3% (76/128) in crizotinib based regimens. ALT/AST mild visual disturbances, nausea, andvomiting were the main side effects. No treatment related death occurred in these crizotinib based treatments.
Conclusions: This pooled analysis suggests that crizotinib based regimens are associated with good responserate and accepted toxicities in treating Chinese patients with EML4-ALK positive non-small-cell lung cancer. crizotinib second-line chemotherapy EML4-ALK positive non-small-cell lung cancer 2015 11 01 4797 4800 http://journal.waocp.org/article_31164_881d7b9d542ce42351dc25ba55be9c92.pdf 2015-11-01 Asian Pacific Journal of Cancer Prevention Asian Pac J Cancer Prev 1513-7368 1513-7368 2015 16 11 Are Rogerofenib and Nilotinib Effective for Advanced Gastrointestinal Stromal Tumor (GIST) Patients who have Already been Given Main Treatments? 2015 11 01 4801 4802 http://journal.waocp.org/article_31165_306ab04cf1eb06a7dab5ad9ac86e346a.pdf