text:
The end of the calendar year means that it is time to recognize the best players in college basketball. HoopsHD's Jon Teitel has reviewed the entire non-conference season and is ready to announce his picks for the 5 best players from each conference based on their all-around stats. Players are listed in a traditional 5-position lineup (G-G-F-F-C) whenever possible with several exceptions, with the best ones in each conference honored as POYSF (Player of the Year So Far). If you think he has overlooked anyone then feel free to post your feedback in the comments section below, and check back later this weekend for the remaining conferences in Part 2.
America East
G: Jairus Lyles (UMBC)
G: Jaleen Smith (New Hampshire)
G: Joe Cremo (Albany)
F: Jahad Thomas (UMass-Lowell)
F: Tanner Leissner (New Hampshire)
POYSF: Jahad Thomas (UMass-Lowell)
G: Rob Gray (Houston)
G: Jalen Adams (Connecticut)
F: Dedric Lawson (Memphis)
F: Obi Enechionyia (Temple)
C: Tacko Fall (UCF)
POYSF: Dedric Lawson (Memphis)
G: Jack Gibbs (Davidson)
G: Jaylen Adams (St. Bonaventure)
G: Matt Mobley (St. Bonaventure)
F: Peyton Aldridge (Davidson)
F: TJ Cline (Richmond)
POYSF: Jaylen Adams (St. Bonaventure)
G: Jerome Robinson (Boston College)
G: Luke Kennard (Duke)
F: Michael Young (Pitt)
F: Bonzie Colson (Notre Dame)
C: Ben Lammers (Georgia Tech)
POYSF: Michael Young (Pitt)
Atlantic Sun
G: Dallas Moore (North Florida)
G: Kendrick Ray (Kennesaw State)
G: Damon Lynn (NJIT)
F: Aubrey Williams (Kennesaw State)
C: Michael Buchanan (SC Upstate)
POYSF: Damon Lynn (NJIT)
Big 12
G: Frank Mason III (Kansas)
G: Josh Jackson (Kansas)
G: Jawun Evans (Oklahoma State)
F: Johnathan Motley (Baylor)
F: Zach Smith (Texas Tech)
POYSF: Frank Mason III (Kansas)
Big East
G: Shamorie Ponds (St. John's)
G: Josh Hart (Villanova)
F: Rodney Pryor (Georgetown)
F: Angel Delgado (Seton Hall)
C: Luke Fischer (Marquette)
POYSF: Josh Hart (Villanova)
Big Sky
G: Jordan Davis (Northern Colorado)
G: Randy Onwuasor (Southern Utah)
G: Tyler Hall (Montana State)
F: Jacob Wiley (Eastern Washington)
F: Bogdan Bliznyuk (Eastern Washington)
POYSF: Bogdan Bliznyuk (Eastern Washington)
Big South
G: Chris Clemons (Campbell)
G: Ahmad Thomas (NC Asheville)
G: Laquincy Rideau (Gardner-Webb)
F: Khris Lane (Longwood)
F: Xavier Cooks (Winthrop)
POYSF: Chris Clemons (Campbell)
Big 10
G: Peter Jok (Iowa)
G: Malcolm Hill (Illinois)
G: Tai Webster (Nebraska)
F: Caleb Swanigan (Purdue)
C: Thomas Bryant (Indiana)
POYSF: Caleb Swanigan (Purdue)
Big West
G: Jaron Martin (UC Irvine)
G: Brynton Lemar (UC Davis)
G: Kendall Smith (Cal State Northridge)
F: Chima Moneke (UC Davis)
F: Tavrion Dawson (Cal State Northridge)
POYSF: Kendall Smith (Cal State Northridge)
G: Denzel Ingram (UNC Wilmington)
G: Kurk Lee (Drexel)
G: TJ Williams (Northeastern)
F: Devontae Cacok (UNC Wilmington)
F: Rodney Williams (Drexel)
POYSF: TJ Williams (Northeastern)
G: Jon Davis (Charlotte)
G: Jon Elmore (Marshall)
F: Erik McCree (Louisiana Tech)
F: Michael Kessens (Florida International)
C: Ronald Delph (Florida Atlantic)
POYSF: Jon Elmore (Marshall)
G: Mark Alstork (Wright State)
G: Cameron Morse (Youngstown State)
F: Alec Peters (Valparaiso)
F: Dikembe Dixson (Illinois-Chicago)
C: Drew McDonald (Northern Kentucky)
POYSF: Alec Peters (Valparaiso)
G: Matt Morgan (Cornell)
G: Steven Spieth (Brown)
G: Bryce Aiken (Harvard)
F: Evan Boudreaux (Dartmouth)
F: AJ Brodeur (Penn)
POYSF: Steven Spieth (Brown)


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Auteur: Philips Price, Morgan
Titel: Dispatches From The Revolution
Sub titel: Russia, 1916-18
A special correspondent for the Manchester Guardian, Price was one of the few Englishmen in Russia during all phases of the Revolution. These pre-censored observations and comments, as well as letters, postcards, and other writings, offer an account of the Russian Revolution from an eyewitness whose political commitment, fluency in Russian, and extensive travel far beyond the cities permitted him to write, uniquely, not only of metropolitan news and politics but also of the experiences and issues significant to ordinary peasants, workers, and soldiers in remote areas of the Russian empire. A foreword by Eric Hobsbawn and introductory material by editor Tania Rose place Price's observations in biographical and historical context.
2000, 195 pag., Euro 9
Duke University Press, Durham, ISBN 9780822320739
This page last updated on: 13-1-2015


text:
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text:
- Research article
- Open Access
A new scoring function for top-down spectral deconvolution
- Qiang Kou1,
- Si Wu2 and
- Xiaowen Liu1, 3Email author
© Kou et al.; licensee BioMed Central. 2014
- Received: 16 May 2014
- Accepted: 11 December 2014
- Published: 18 December 2014
Top-down mass spectrometry plays an important role in intact protein identification and characterization. Top-down mass spectra are more complex than bottom-up mass spectra because they often contain many isotopomer envelopes from highly charged ions, which may overlap with one another. As a result, spectral deconvolution, which converts a complex top-down mass spectrum into a monoisotopic mass list, is a key step in top-down spectral interpretation.
In this paper, we propose a new scoring function, L-score, for evaluating isotopomer envelopes. By combining L-score with MS-Deconv, a new software tool, MS-Deconv+, was developed for top-down spectral deconvolution. Experimental results showed that MS-Deconv+ outperformed existing software tools in top-down spectral deconvolution.
L-score shows high discriminative ability in identification of isotopomer envelopes. Using L-score, MS-Deconv+ reports many correct monoisotopic masses missed by other software tools, which are valuable for proteoform identification and characterization.
- Mass spectrometry
- Deconvolution
- Software
In the last two decades, bottom-up mass spectrometry (MS) has been the mainstream of proteomics analysis [1–4]. Although it is efficient and high-throughput for protein identification and quantification, bottom-up MS has its limitations. It involves a sample preparation step in which long proteins are digested into short peptides by proteases, reducing its ability to identify various proteoforms with multiple changes, such as mutations, post-translational modifications (PTMs), and degradations [5, 6]. In contrast, top-down MS analyzes intact proteins, making it the method of choice for complex proteoform identification.
In a mass spectrum, each peak is represented as (m/z,i n t e n s i t y), where m/z and intensity are the mass-to-charge ratio and abundance of its corresponding ion, respectively. Because of the existence of natural isotopes, ions of the same chemical formula and charge state have different m/z values and correspond to a list of spectral peaks in a mass spectrum, called an isotopomer envelope. The monoisotopic mass of an ion is the sum of its atomic masses using the most abundant isotope for each of its atoms.
Compared with bottom-up mass spectra, top-down mass spectra are more complex because they often contain many high charge state isotopomer envelopes, some of which overlap with one another [7, 8]. As a result, a key step in top-down spectral interpretation is to deconvolute a complex top-down mass spectrum to a list of monoisotopic masses.
Given the chemical formula and charge state of an ion, its theoretical isotopomer distribution can be calculated based on the frequencies of natural isotopes. When the chemical formula is unknown and the only available information is its monoisotopic or average mass, the well-known averagine model [9] can be used to estimate the chemical formula from the monoisotopic or average mass. A theoretical isotopomer distribution is represented as a list of theoretical peaks (m/z,p r o b a b i l i t y), in which m/z and probility are the mass-to-charge ratio and probability of the corresponding isotopomer. In top-down spectral deconvolution, theoretical isotopomer distributions are utilized to identify and group isotopic peaks.
Spectral deconvolution of profile mass spectra has been studied by several groups [7, 10]. In this paper, we focus on centroided spectra. While profile spectra keep all information of raw data, centroided spectra simplify data representation and speed up spectral deconvolution. Similar to mass spectra, isotopomer distributions can be represented in the profile or the centroided mode (Additional file 1: Figures S1 and S2). The centroided mode will be used in the proposed scoring function.
Many software tools have been developed for top-down spectral deconvolution [7, 10–12]. Most tools deconvolute a top-down mass spectrum in four steps. First, candidate isotopomer envelopes are extracted from the experimental spectrum and matched to theoretical isotopomer distributions. Second, the theoretical isotopomer distribution in a match is converted into a theoretical isotopomer envelope by scaling the probabilities to theoretical peak intensities. The scale ratio is estimated based on the peak intensities of the experimental isotopomer envelope. Third, the matches are evaluated by a scoring function, and a match is reported only if its score is higher than a specified threshold. Finally, a monoisotopic mass is obtained from each of the reported isotopomer envelopes.
The scoring function for evaluating experimental isotopomer envelopes determines the accuracy and sensitivity of spectral deconvolution. Designing a good scoring function is a challenging problem because complex mass spectra often contain many noise peaks and overlapping isotopomer envelopes. Most software tools use scoring functions based on the intensities of peaks in a pair of experimental and theoretical isotopomer envelopes, such as the sum of squared errors of peak intensities [7], the ratios of neighbouring peak intensities [13], and the dot product of intensity distributions [12]. The scoring function in MS-Deconv [8] combines peak intensities and errors in m/z values.
In this paper, we present a new scoring function, L-score, for computing the similarity between a pair of experimental and theoretical isotopomer envelopes. L-score can be used independently for spectral deconvolution or combined with other spectral deconvolution tools for envelope selection. We developed a software tool, MS-Deconv+, by combining MS-Deconv and L-score. Experiments showed that MS-Deconv+ outperformed other existing software tools in top-down spectral deconvolution.
Data sets
A data set from Salmonella typhimurium (ST) [14] was used for training and testing L-score. Cell lysate obtained from ST was analyzed with a C4-based high-performance liquid chromatography (HPLC) column coupled with an LTQ-Orbitrap mass spectrometer. A total of 4,636 collision induced dissociation (CID) tandem mass spectra were acquired. The charge states of the spectra range from 1 to 24; the precursor masses of the spectra range from 1,000 to 20,000 Dalton (Da). (See Ref. [14] for the detailed experimental procedure.)
Two Escherichia coli (EC) data sets were utilized to test L-score and MS-Deconv+. Cell lysate of EC was analyzed by a reversed phase liquid-chromatography (RPLC) coupled with an LTQ-Orbitrap Velos mass spectrometer. A total of 3,704 higher-energy C-trap dissociation (HCD) and 4,045 electron-transfer dissociation (ETD) tandem mass spectra were collected at a resolution of 60,000.
Theoretical and experimental envelopes
Since the proposed scoring function is designed for centroided data, only centroided isotopomer distributions and centroided mass spectra are studied. In a centroided isotopomer distribution, two isotopomers are treated as the same if they contain the same number of neurons. For example, a water molecule with two 1H atoms and one 18O atom and another water molecule with two 2H atoms and one 16O atom are treated as the same although their masses are slightly different. As a result, isotopomers with the same number of neurons are represented by one peak. When the charge state of an ion is z, the distance between two neighbouring peaks in its centroided theoretical isotopomer distribution is approximately 1.00235/z thomson (Th) [7].
Top-down mass spectra contain some noise peaks. The noise intensity level of a spectrum is estimated by plotting the histogram of the peak intensity distribution and assuming that it is in the intensity bin with the largest number of peaks [7]. A peak is considered as a signal peak if its intensity is higher than the noise intensity level. In addition, a charge state is valid if it is no larger than a user-defined parameter.
Given a theoretical envelope, a list of experimental peaks is extracted from the mass spectrum to form its corresponding experimental envelope by matching each peak in the theoretical envelope to an experimental peak with a similar m/z value (within an error tolerance). If such an experimental peak is not found, we add into the spectrum a zero-intensity peak whose m/z value is equal to the theoretical peak. A theoretical envelope and its matched experimental envelope are called an envelope match.
Training and test data sets
We generated and annotated a set of envelope matches from the ST data set for training and testing L-score. In short, after tandem mass spectra were identified by database search, the resulting protein-spectrum-matches were utilized to obtain annotated envelope matches. The detailed steps are described below.
ReAdW (http://tools.proteomecenter.org/software.php) was used to convert the Thermo raw file into a centroided mzXML file. MS-Deconv [8] was applied to extract a list of monoisotopic masses and their corresponding envelope matches from each tandem mass spectrum of the ST data set. The deconvoluted mass lists were searched against a target-decoy concatenated ST proteome database using MS-Align+ [15]. Default parameter settings were used in MS-Deconv and MS-Align+. The Benjamini-Hochberg procedure [16, 17] was employed to estimate false discovery rates (FDRs) for identified protein-spectrum-matches. When the E-value cutoff was 5.74×10-4, a total of 493 target protein-spectrum-matches were identified and no decoy protein-spectrum-matches were reported. We assume the 493 target protein-spectrum-matches are all correct because they have an estimated 0% spectrum level FDR. Of the "correct" identifications, 468 protein-spectrum-matches, from 83 proteoforms of 67 proteins, do not contain PTMs (some may have truncations).
Since the PTM localization problem has not been fully solved in top-down spectral analysis, we used only the 468 protein-spectrum-matches without PTMs to generate training and test envelope matches. For each of the 83 proteoforms, we selected only one identified spectrum with the largest number of monoisotopic masses to remove similar spectra. The resulting 83 spectra contained 7,995 envelope matches. If the monoisotopic mass of an envelope match was mapped to a theoretical fragment ion of the identified proteoform within 15 parts per million (ppm), the envelope match was labeled as "correct", otherwise, "incorrect". Since the data set contains only CID tandem mass spectra, b- and y-ions as well as b- and y-ions with neutral losses (b-H 2O, b-N 3H, y-H 2O, y-N 3H) were used for labeling envelope matches. In addition, ±1 Da errors were allowed in mapping monoisotopic masses of envelopes to theoretical fragment ions because they are common in extracting monoisotopic masses from isotopomer envelopes. Out of the 7,995 envelope matches, 3,726 were labeled as "correct", and 4,269 were labeled as "incorrect".
L-score uses several features whose computation involves the number of peak pairs in an envelope match. Thus, we divided the 7,995 envelope matches into 4 groups with 2, 3, 4, and ≥5 peak pairs, which contained 924,1,284,2,017, and 3,770 envelope matches, respectively. The envelope matches in each group were randomly divided into training and test envelope matches of the same size. (If one group contains 2n+1 envelope matches, where n is an integer, the training data set contains n envelope matches and the test data set contains n+1 envelope matches).
We also generated a test set of envelope matches from the EC HCD data set. Following the method for the ST data set, we identified 1,537 protein-spectrum-matches with an estimated 0% spectrum level FDR, including 625 protein-spectrum-matches without PTMs from 242 proteoforms of 109 proteins. For each of the 242 proteoforms, we chose a matched spectrum with the largest number of monoisotopic masses. Finally, a set of 27,091 envelope matches was obtained, including 9,744 "correct" and 17,347 "incorrect" ones. They were further divided into 4 groups with 2,3,4, and ≥5 peak pairs, which contained 1,535, 4,572, 3,894, and 17,090 envelope matches, respectively.
Features of envelope matches
Let S be an experimental mass spectrum. A peak in an isotopomer envelope is represented by a pair (x,y), where x and y are the m/z value and intensity, respectively. Let E=(x1,y1),(x2,y2),⋯, (x k ,y k ) be a theoretical envelope where x1<x2<…<x k , and E′=(x1′,y1′),(x2′,y2),⋯,(x k′,y k′) its corresponding experimental envelope in S. Each theoretical peak (x i ,y i ) is mapped to the experimental peak (x i′,y i′) for 1≤i≤k. Below we describe five features for distinguishing correct envelope matches from incorrect ones.
Peak intensity distributions
Supporting envelopes
Neutral loss envelopes
In the implementation of L-score, the envelope detection and selection methods in MS-Deconv are used to generate candidate envelope lists, in which supporting envelopes and neutral loss envelopes are identified.
Missing peak numbers
Peaks may be absent from experimental envelopes. In the generation of candidate envelopes, an experimental envelope is removed if it has ≥3 missing peaks, 3 theoretical peaks and 2 missing peaks, or 2 theoretical peaks and 1 missing peak. In addition, an experimental envelope is removed if it does not contain k-3 consecutive matched peaks, where k is the number of peaks in the theoretical envelope. As a result, most missing peaks in experimental envelopes are at the ends of isotopomer distributions. Therefore, locations of missing peaks are not included in L-score. Since envelope matches without missing peaks have a higher accuracy rate than those with missing peaks (Additional file 1: Figure S6), we introduce another feature m(E′) to represent the number of missing peaks in an experimental envelope E′.
The scoring function
The weights of the features in L-score reported by logistic regression using the ST training envelope matches
#peak pairs =2
#peak pairs =3
#peak pairs =4
#peak pairs ≥5
M/z distance
Intensity distribution
#supporting envelopes
#neutral loss envelopes
#missing peaks
To compare envelope matches from different peak pair number groups, we trained a lookup table for each peak pair number group to convert raw scores L(E,E′) to local FDRs using the ST training data set. Given a raw score and a peak pair number group, we count the numbers of correct and incorrect envelope matches in the training group whose scores are similar to the given score (the bin size is 0.02) and use the two numbers to estimate the local FDR. In practice, candidate envelope matches of a top-down mass spectrum are ranked and selected based on their estimated local FDRs.
Combination of MS-Deconv and L-score
MS-Deconv deconvolutes top-down mass spectra with four steps. First, a list of envelope matches is generated by enumerating all valid charge states and all signal peaks in a mass spectrum as base peaks. Second, all envelope matches are filtered based on the number of missing peaks and the number of consecutive matched peaks. Third, a graph model is employed to select a small number of envelope matches from the list that can explain the spectrum well. Fourth, the number x of envelope matches to report is specified by the user or estimated by the precursor mass when a tandem mass spectrum is analyzed. (When the precursor mass is M, the length L of the target protein is estimated as ⌈M/mavg⌉, where mavg is the average mass of the 20 amino acid residues, and the number of envelope matches to report is estimated as 2(L-1).) The envelope matches selected in the previous step are ranked by their similarity scores, and the top x envelope matches are reported. Finally, monoisotopic masses are extracted from the top x envelope matches. The similarity scoring function used in MS-Deconv is referred to as M-score.
To combine L-score with MS-Deconv, M-score is replaced by L-score (and the local FDR) in the fourth step of MS-Deconv (Additional file 1: Figure S7). By combining MS-Deconv and L-score, we developed a new software tool, MS-Deconv+, for top-down spectral deconvolution. Since local FDRs are reported with L-scores for envelope matches in MS-Deconv+, a local FDR threshold can be specified to decide the number of envelope matches to report.
In practice, MS-Deconv+ with default weights of features is first used to analyze data sets that are different from the training data set. To further improve the performance of MS-Deconv+, MS-Align+ can be utilized to identify highly confident protein-spectrum-matches and generate a set of training envelope matches to train the weights of features.
Results and discussion
We implemented L-score and MS-Deconv+ in Java and tested them on the ST and EC data sets.
Comparison of distance functions for peak intensity distributions
Discriminative abilities of single features and L-score
Comparison with other scoring functions
Combination of L-score and Decon2LS
Decon2LS [11], a reimplementation of THRASH [7], reports a list of ranked envelope matches from a top-down mass spectrum. To test L-score coupled with Decon2LS, L-score was utilized to re-rank the envelope matches in the list reported by Decon2LS. Two lists of ranked envelope matches (one by Decon2LS and the other by L-score coupled with Decon2LS) were generated for each of 242 mass spectra in the EC HCD test data set. For each i=1,2,…,20, we collected two sets of envelope matches with the rank i from the lists of ranked envelope matches reported by Decon2LS and L-score coupled with Decon2LS and then compared their accuracy rates (Additional file 1: Figure S8). L-score coupled with Decon2LS reported more correct top ranked enveloped matches than Decon2LS. In practice, when Decon2LS reports x envelope matches from a mass spectrum, the following procedure can be used to boost the accuracy rate of reported envelope matches. The RL-value threshold of Decon2LS is lowered so that the number of envelope matches extracted from the spectrum is larger than x. Then L-score is utilized to re-rank the envelope matches, and only the top x ones are reported.
Comparison of Decon2LS, MS-Deconv and MS-Deconv+ on spectral identification
In this paper, we proposed L-score for evaluating experimental isotopomer envelopes, which outperformed existing scoring functions in distinguishing correct experimentalenvelopes from incorrect ones. We further developed MS-Deconv+, a top-down spectral deconvolution tool that combines MS-Deconv and L-score. In the experiments on the two EC data sets, MS-Deconv+ reported more correct monoisotopic masses than MS-Deconv. These correct monoisotopic masses provide essential information for proteoform identification and characterization.
Publication of this article was funded by a startup fund provided by Indiana University-Purdue University Indianapolis.
This work was supported by a startup fund provided by Indiana University-Purdue University Indianapolis. Portions of this work were supported by funds from EMSL intramural research project and performed at EMSL, a national scientific user facility sponsored by DOE-BER and located at Pacific Northwest National Laboratory (PNNL). PNNL is a multi-program national laboratory operated by Battelle for DOE under Contract DE-AC05-76RL01830.
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


text:
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Exceed And Excel enjoyed a very successful Saturday on 30 June, adding not one, but two new Black Type winners to his record.
Two-year-old filly Royal Intervention certainly looks like one to watch in the future as she utterly dominated in the Listed Empress Fillies' Stakes on Newmarket's July course.
Soon in front under Gerald Mosse, the Ed Walker-trained filly was in command throughout and once asked to quicken a furlong from home, eased clear to take the six-furlong contest by four and a half lengths.
Slade Power's promising daughter Strings Of Life, who became her sire's very first winner back in April, gained her first Black Type when taking second.
Royal Intervention was bred by the Exciting Times Partnership and is out of Exciting Times, making her a half-sister to four other Black Type performers including G1 Beverly D Stakes winner Gorella.
Later on the same day, three-year-old colt Julio recorded his first Group win when taking the G3 Hamburger Flieger-Preis by a head from Celebrity (Shamardal).
Bred by Gestut Brummerhof, Julio is the first foal to race out of the Stakes-placed Beat Hollow mare Julissima, who hails from the family of Melbourne Cup hero Rekindling.
Exceed And Excel is now sire of 11 Stakes winners so far this year, including multiple Group winner Heavy Metal and smart filly Threading, winner of last year's G2 Lowther Stakes and second in the G1 Coronation Stakes at Royal Ascot last time out.


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Training and Placement at Central University of Jharkhand, Ranchi
Abundance of skilled and effective manpower availability is key to trigger developmental pace in the society. Today, education is not just dissemination of knowledge, but equipping the students with appropriate skills and ability to take up new challenges in Industry environment for contributing to the society immensely as well as fulfilling their career dreams.
Central University of Jharkhand is imparting education to over 2000 students in different integrated and innovative programs spanning from Performing Arts to Humanities and Engineering. Several new generation and innovative programs on different streams are also being conducted. The programs are designed in such a way so as to equip the participants to find their respective role in real life situations and enable them to face the challenges that pose. Students of most of the professional programs of the University viz., 5 years Integrated M.Tech, M.Sc and M.A are getting placed in the Campus recruitments.
|Student Placement web-portfolio of Final Year Integrated M.Tech Students, Department of Energy Engineering
Campus Placement opportunity for the post of Program Officer -Energy , Program Officer - Clean Air in Centre for Environment and Energy
Result of Written test in CUJ to recruit for several Associate Posts on 26th Feb 2018
Notice for Campus Selection - Azim Premji Foundation will be in our campus to recruit some of our students for entry level Associate Posts on 26th Feb, 2018
Placement Brochure for Centre for Energy Engineering(2013-18)
Placement Brochure for Centre for Business Administration (2013-18)
Written Exam Result of Campus Selection for Associate Posts by Azim Premji Foundation in CUJ
Azim Premji Foundation written test will be held on 8th February 2017 at 10:00 am at CUJ Campus
Azim Premji Foundation will be in our capmus to recruit M.A / M.Sc final (10th Semester) Student in 1st week of feb 2017,Last date to submit resume is 31th January 2017
Placement Brochure for Centre for Energy Engineering(2016-17)
Placement Brochure for Centre for Business Adminsitration (2016-17)
Placement Brochure for Centre for Business Adminsitration (2015-16)
Placement Brochure for Centre for Energy Engineering
Centre for Land Resource Management Pass Out Students Job Status
Placement Brochure for Centre for Business Adminsitration
Centre for Land Resource Management
Notification for Placement Cell at CUJ
Office Order - Constitution of Placement Cell in Central University of Jharkhand
For Placement queries please contact - Dr. Devdas B.Lata , Email Id - email@example.com, Mobile No - 9284795918, Coordinator - Placement Cell


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CNC Aluminum Upper Receiver
A&K IU-MASADAB Masada Auto Electric BB Gun Airsoft Metal Gear
A&K IU-MASADAB Masada Auto Electric BB Gun Airsoft Metal Gear
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Steel Gears and Metal Gear Box
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Foldable and retractable polymer stock
300 round high capacity P-Mag
Flip up front and rear sights
Bolt lock for easy hop up adjustment
Adjustable hop up
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Package Includes: 300 round high capacity Magpul P-Mag is also included. Charger and battery sold separatly.
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Laser Genesis patients report excellent results over their entire faces and necks in such conditions as Rosacea. Additionally, practitioners may suggest this therapy to diminish recent scars on other parts of the body.
Diffuse redness is a general appearance of facial redness, such as rosy cheeks. This condition is caused by dilated capillaries, resulting in an appearance of general redness, not discrete, well-defined vessels. The most commonly affected areas are the nose, forehead and cheeks. Diffuse redness is often associated with a condition called Rosacea and it can be hereditary. Laser Genesis will help your appearance by gently treating and reducing this condition.
Patients often describe the treatment as relaxing and therapeutic. You will experience a gentle warming of your skin's surface during the procedure as the laser handpiece is moved back and forth about 1/2 inch above your skin. You can immediately return to normal activities, utilizing sunscreen if participating in outdoor activities
By gently heating the upper dermis well below your skin's surface, Laser Genesis stimulates collagen regrowth. Additional heat is generated in dilated capillaries to reduce redness.
You will not need gel, anesthetic cream or ice prior to treatment. Before the actual Laser Genesis treatment, you will be asked to remove your make-up or moisturizers and, in some cases, you may be asked to shave the area to be treated. Our medical staff will review and assist with all pretreatment requirements.
The treatment results are often subtle. On average, you may require four to six treatments in order to achieve optimal results. Every patient's condition and needs vary. For more information about your expected results or the number of treatments necessary, consult our medical staff.
Although most patients report few if any side effects, the most common is a slight redness that disappears within a few hours of treatment. Consult our medical staff for complete information about the benefits and risks of treatment.
Over the course of four to five treatments, Laser Genesis can help restore the skin's youthful glow and appearance by smoothing out uneven textures caused by aging, sun damage or scars. You will see a more creamy, healthy complexion as large pores are reduced in size, and minor imperfections are diminished. The individual treatment results are subtle, but the overall effect of multiple treatments can be quite dramatic.


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8th February
Here is a somewhat "one-off" strain that I came across sometime last year. I called this strain "one-off" because it didn't seem to catch on, was more like an experiment. It was clear that traits from God's Gift and Bubba Kush were coming through in this hybrid. It had the skunky and piney kushey-ness of bubba, AND the sweet grape smell you'd find in God's Gift. It's interesting because God's Gift is about the most dense strain I can think of, and Bubba is somewhat looser and more leafy. Check out this picture, medical marijuana has never and will never look this good again!


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Hi, I'm Vanessa a 20 something Portuguese/ Canadian /Venezolana living in London with a love affair for all things design.
After graduating from a languages degree from the University of Manchester I decided to take a leap of faith and pursue my passion for design by studying an Interior Design Diploma at London College of Communication. Since qualifying, I have undertaken several residential projects, worked as a bespoke furniture designer and am currently a Design Assistant for exhibitions, showrooms and furniture at Designers Guild.
I launched Design88 in 2011 to have a dedicated platform to showcase my portfolio and that of other aspiring designers. It was unfortunate to graduate under the cloud of recession and I felt it an important time to promote young emerging talent!
I soon realised that this also felt like the perfect place to share the things that inspired me. Lo and behold, I began writing little articles that made me happy and, apparently, some of you too.
I post as often as I can and dedicate most my entries to cover design related topics from architecture, interiors, product design, trade shows, home improvements, design trends etc..However, you might find the odd post relating to lifestyle such as food and travel, other topics that I happen to be a big fan of!
I love reading your comments so please let me know your thoughts or email me: at: email@example.com
All photographs have click- through links that connect them to their original owner. I do not claim to own any of the featured images in my posts unless stated otherwise. Likewise, if I have re- blogged a photograph I am not responsible for the credit.
If I have displayed some of your work and you would like it taken down you can send me a message and I will be glad to remove it.
*Please note: Design 88 does not accept free samples, products or compensation for editorial coverage unless otherwise stated*


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Posted by Sean Dallas on May 01, 2019
5 3 Vortec Wiring Harness
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5 3 vortec wiring harness Advance adapters offers brand new completely assembled gen iii vortec v howell harnesses in every swap weve done and have never had a problem. Howell sent a gm computer and harness that work The combination shown here is a 4.8 liter vortec truck engine which is the smallest and typically the most affordable ls based engine available. The 4.8l truck engine has several big brothers 5.3l . How does a junkyard 5.3l gen iii small block boosted to 550 hp with a do it yourself turbo setup built for 3069 suit your fancy that tally includes a tuned factory computer a wiring harness.
It's possible to get or download caterpillar-wiring diagram from several websites. If you take a close look at the diagram you will observe the circuit includes the battery, relay, temperature sensor, wire, and a control, normally the engine control module. With an extensive collection of electronic symbols and components, it's been used among the most completed, easy and useful wiring diagram drawing program. 5 3 Vortec Wiring Harness. The wiring diagram on the opposite hand is particularly beneficial to an outside electrician. Sometimes wiring diagram may also refer to the architectural wiring program. The simplest approach to read a home wiring diagram is to begin at the source, or the major power supply. Basically, the home wiring diagram is simply utilized to reveal the DIYer where the wires are.
If you can't locate the information, get in touch with the manufacturer. The info in the diagram doesn't indicate a power or ground supply. The intention of the fuse is to safeguard the wiring and electrical components on its circuit. A typical watch's basic objective is to tell you the good time of day. When selecting the best type of computer cable to fulfill your requirements, it is very important to consider your upcoming technology plans.
Installing a tachometer on your Vehicles can assist in preventing critical repair problems, however. You might have a weak ground issue. The way the brain learns is a subject that still requires a good deal of study. How it learns can be associated by how it is able to create memories.
In a parallel circuit, each unit is directly linked to the power supply, so each system gets the exact voltage. There are 3 basic sorts of standard light switches. The circuit needs to be checked with a volt tester whatsoever points. 5 3 Vortec Wiring Harness. Each circuit displays a distinctive voltage condition. You are able to easily step up the voltage to the necessary level utilizing an inexpensive buck-boost transformer and steer clear of such issues. The voltage is the sum of electrical power produced by the battery. Be sure that the new fuse isn't blown, and carries the very same amperage.
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The bulb has to be in its socket. Your light can be wired to the receiver and don't require supply additional capacity to light as it can get power from receiver. In the event the brake lights aren't working, a police officer may block the vehicle and issue a warning to create the repair within a particular time limit. Even though you would still must power the relay with a power source or battery. Verify the power is off before trying to attach wires. In case it needs full capacity to begin, it won't operate in any way.
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Kaveri Falls. - View from the pool at the foot of Bar Chukki.
Photographer: Unknown
Medium: Photographic print
Date: 1890
This photograph of the Kaveri Falls, Mysore taken in the 1890s by an unknown photographer, is from the Curzon Collection's 'Souvenir of Mysore Album'. A note with the photograph gives the following details, "A project to utilise the power of the falls, for the production of electricity, is now in the course of execution and is calculated to supply the Kolar Gold Fields with 4,000 H.P. The works are expected to be completed in eighteen months at a cost of £280,000." These falls are also known as the Sivasamudram Falls.


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On Saturday evening, we went to Sidecar in South Park Slope, Brooklyn. It was oh so totally yummy and I would definitely go back. I loved the menu because it was a small list and then I know that the kitchen won't be overwhelmed. I hate big menus!
There were nine of us, so we split several small plates for the table: Chicken Liver Pate on Crostini to die for, a salt cod Brandade spread, Bean Dip with bacon, and a special of Gravlax. Seven of us ordered the Fried Chicken which was to die for. I don't usually order fried chicken unless it is really good (crispy and moist) and I would definitely order it again. It was served with kale with bacon and mashed roots, both perfectly prepared. Totally delicious. Two others ordered the sidecar sandwich and I had a bite of that and it was also worthy of praise. We finished off the evening splitting chocolate pudding and apple cobbler. Excellent! I would go back.
We waited on the patio for our table.


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WHERE: 225 N. 38th St. parking lot
This festival is the celebration of LoLa 38's yearlong project to highlight arts, culture and community along Lancaster Avenue and West Philadelphia. The stage will feature over five hours of live shows from local dance ensembles, musicians, performing artists and a headliner to be announced. Food artisans, visual artists and craftspeople will sell their creations, and the festival will also include an all-ages fun zone.
COST: Free, but tickets are required


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text: Maddeline received her esthetician's license from Bluegrass Community and Technical College. She is an advocate of daily skin health, and her goal is to help people achieve optimal results from treatments. Maddeline's favorite service to perform is dermaplaning. She loves the glowy results clients receive, especially when paired with a chemical peel or HydraFacial. Her philosophy is: regardless your age, it is never too late to achieve your perfect skin.


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I'm so tired.
I have been taking a medication since getting the diagnosis. And it just makes me so tired. I take it before bed, but I am still wrecked in the morning. One coffee won't do. I feel like I've had a handful of Gravol or Benadryl. That groggy.
I'm a Mom. I'm a breastfeeding Mom. This little pill is compromising what I want to accomplish as a mother. Or just as a person.
I can't pump (express the milk) after the medication is taken as it is not the best for baby. So, I have to wait until morning to pump again. And if I could get up at 5:00 am then maybe it wouldn't be so bad. But this little pill has me so groggy and exhausted, I simply cannot get out of bed. As a result, hours go by and that's signaling my body to make less milk. So, now I am stressed out about that too and the inability of not having enough food for my baby (don't worry, he is supplemented with formula, he is one well fed baby!).
I didn't take the pill the other night, and when I woke up at 5:00 am I felt refreshed and rejuvenated. I had so much energy. I got so much done. Like, SO MUCH. I felt like SuperMom! I had my energy back and it was wonderful. The down side? I noticed the OCD and anxiety so much more.
Truth be told, I didn't think there would be much of a difference. When I had started the medication, I felt like I didn't notice much improvement in symptoms. So, when I realized that I did not take it, I assumed my day would be like any other. Wrong.
I was indeed full of energy. But I was also full of unwanted thoughts, worry and angst. It was paralyzing at times. I must've checked my heart rate 150 times.
It doesn't help that my POTS (Postural Orthostatic Tachycardia Syndrome) (heart arrhythmia) is acting up. I'm now 8 weeks postpartum so my water and blood volume are going back to normal following pregnancy, making my POTS worse. Also, it's been hot AF here the past few days. I am very intolerant to heat with my condition and high heat aggravates my heart a lot. So, there I was with a wonky heart and anxiety and the compulsion of measuring my heart rate repeatedly all mushed together. Blech.
Without the medication I was fully aware of what was going on and how much I obsessed over my heart, even though I knew I was going to be fine.
I've also been noticing these compulsions in everything else I do. It's really sending me through a loop. I hate it. I see myself doing all these things and I don't know what's me and what's the OCD. I like to make lists. My lists have lists. I love them. But I've been so scared of lists since finding out I have OCD, that I have been avoiding them. Being told this thing I love to do is part of a mental illness really spooks me.
I've always turned every pop tab on any pop can ever 90 degrees. Always. I never thought of it as weird, just something I did. I always knew which pop was mine? Now, realizing I couldn't drink my pop without the tab turned to the side, there's another compulsion to add to my lists. Oh, my lists.
I count everything I do. I just thought this was a way to occupy my brain. I don't usually count things to a certain number, I just count everything until I am done the task. Things like how many times I stir my coffee, how many steps from my car to the store, how many brush strokes of my hair until I am satisfied, things like that. But now I find out that that's an indicator of OCD? It feels like everywhere I turn here's a problem with my brain. And I just don't like it. I can't tell where I end and where the OCD begins. Are we one in the same? Maybe. But that makes me feel uneasy.
If I ever get this under control and "fixed" as it were, what will be left over? Will I still like me? Who am I?
Do I take this medication and sacrifice the best food source for my baby? Or, do I not and have loads of energy but be in a constant state of angst?


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some things we learned from proper magBack
19 Oct
Jennifer Lee O'Brien
Here we are, at Proper Mag HQ. It's late August, and the weather is just so average; grey skies, bland temperature. Sort of cold, but also, not.
Inside Proper Mag's office it is dead warm. The heating is cranked right up. We should all be drinking watered-down margaritas from plastic fish bowls, because the climate in this red-bricked office conversion is so faux tropical. If their heating bill is any indication, the team at Proper Mag are doing really, really well. And we should all be glad.
Proper Mag is a print magazine produced by the funniest lads (of Stockport) of all time: Mark Smith and Neil Summers. Mark and Neil launched Proper as a website in 2002. Back then it was called ProperTop.com. They reviewed gigs, club nights and albums and wrote articles like "How To Be Happy In A Sad Sad World" and quizzes like, "Are You Saddam Hussein?" They took the piss (a lot) — and they were really good at it.
ProperTop got so popular they made a fanzine out of it called Proper Mag. They've since released twenty-three issues and now run a website and creative agency working with brands like Levi's, Barbour and adidas. They sell mugs and apparel, like socks, sweatshirts and t-shirts, and have recently launched their own brand called Hikerdelic. This has been a full-time job for Mark and Neil since January 2016.
"I never thought we'd end up doing this," says Mark. "Especially when we first met."
"When our eyes met across a crowded call centre," says Neil.
"You had long hair," says Mark.
"I asked if I could borrow Mark's Pritt Stick. You know, a glue stick. But it was actually his asthma inhaler," says Neil.
"Which I've got somewhere."
"We should have framed it."
Mark picks up a bag next to his desk and begins to search for it.
"It does look like a Pritt Stick, to be fair," says Neil.
We spent an hour with Mark and Neil in the very lush climate of Proper HQ. Here are some things we learned from them.
"I'm not really into football," says Neil. "I was into this thing called 80s Casuals. Most people would argue that the whole Casual scene came out of football, and that is purely about football. All my mates were into clothes but none of them had any interest in football; we were more influenced by American breakdancing. The kids that used to hang out around the Arndale Centre in Manchester."
"I've grown up watching and playing football," says Mark. "It starts with what boots you get at like, seven or eight years old. Your loyalty to that brand begins; and then you're ten or twelve years old, and you want a tracksuit, or a jacket or whatever. It just carries on from there."
"Where we're from, Stockport, is like the headquarters of adidas in the UK," says Neil. "So everyone in Stockport, by default, buys adidas."
"I think everyone knows someone who can get into the factory," says Mark.
"I probably didn't wear Nike until I was in my late twenties," says Neil. "It's all a bit hazy. We used to go to this club called the Hacienda, which everyone always bangs on about as being this Mecca. And it was for a while. I remember one night we were there and we were all dressed like we were going to get on a yacht. All yachting jackets, and probably baggy jeans and trainers and stuff. Generally, we were a bit scruffy. Massive coats. Again, that's the football thing, innit?" he says to Mark. "If you're sat watching a match for ninety minutes in December, or January, it's seriously cold."
"I think that's where the coat thing comes from," adds Mark. "People always talk about the rain. But it's really cold at times."
"Rain coats are going to always be at the heart of the Mancunian look because it pisses it down," says Neil. "And there's that working class thing of wearing the most expensive stuff you can find. You might live in a council house, but you've got a five hundred quid Stone Island jacket to walk around in. It's about being flash, innit."
"You can get really into the politics of it," says Mark. "I think it's people wanting to punch above their weight and break the mould of whatever society wants them to be — whether that's wearing a golfing jacket to twist people's head, or a Stone Island jacket or trainers that nobody else has got. I think things are a bit different now with the internet."
"I wonder what kids do now compared to what I did," says Neil, "I was eighteen in 1990, which is when Manchester was massive. I used to go out on a Thursday night and come back on a Tuesday morning. But now, people don't really go nightclubbing do they?"
"What you've got to remember is that was when there was like four TV channels. No internet," says Mark. "And people were less bothered about their health, things like that. It's a different world now."
"The internet has ruined everything, innit?" says Neil. He pauses, and then adds: "people must still go out taking drugs."
"We worked together in a call centre. I started there in 1999," says Neil. "We just had a lot of fun messing about. When you've got a shit job and you're working shit hours, you either leave quite soon or you — "
"Carve out a little tiny comfort zone," says Mark.
"It brings out the humour as well, doesn't it?" says Neil. "That's a coping strategy, innit. Especially around here. Rather than getting therapy, just take the piss. Get drunk."
"If the internet had been where it is now, we probably wouldn't have began Proper," says Mark. "Social media wasn't what it was now, it was just message boards and forums, really. It was something we did for a laugh; and we did it for a laugh for quite a long time. And then people started sending us things and started asking us to do things."
"So, when we started off there were magazines like FHM and Arena — all those kinds of mags," says Neil. "And they were just really London, really snooty, really elitist. Twenty grand watches. Reviewing Aston Martin's and stuff. That just doesn't happen in Stockport; we don't drive around in Aston Martin's in suits. That's why we made Proper. We were big fans of Loaded and The Face magazine in the '90s. But it sort of ceased to exist in the noughties. So we made something for us. It was something we liked and wanted to read."
"I think I sold the first three for about twelve quid," says Mark. "It was a pack to make it worthwhile. And then for every one after that it was like, three pounds fifty — something like that. We sold it to people on forums. They fed into what we wrote about as well."
"Issue 10, that was the first proper magazine," says Neil.
"It was as good as it was going to get by me designing it and both of us writing," says Mark. "It needed somebody to oversee it and show us how to make a real magazine. So we did two issues with James Brown who founded Loaded magazine, which was nice."
"We thought we had made it," says Neil. "Thought we could retire soon."
"And then James Brown just got busy with Sabotage Times and we just carried on. And we've carried on ever since," says Mark.
"We're still making something we'd be happy to read," says Neil. "It's quite organic. Brands will come to us and ask us to write about stuff, but ninety percent of the time it's just Mark and I sat here going, 'what should we write about?
He turns to Mark, and says, "it was never intentionally a clothes-based magazine, was it? It's just sort of gone that direction. There's no agenda, really. We've always sort of stuck to having a laugh, haven't we? And not being fashion wankers."
"When we first went down to London, we were like the little country mice in the big city, meeting all these fashion people wearing neckerchiefs and stuff," says Neil. "I think northerner's have always got this thing about London — how everyone is dead unfriendly and horrible. But every time we've worked down there, it's been the opposite. Everyone's dead nice."
"That first trip, we got down there. I felt like, this is not our world, this is not where we fit in, they're going to find us out," says Mark. "I think by the end of that day, we felt like we'd intimidated more people than had intimidated us."
"To be fair, you were walking around with a screwdriver and fresh stitches in the head," says Neil.
"It just opened the door and made us realise that it was possible to do this more than we had been doing it," says Mark.
"The most annoying people in London are all northerners who've moved down there," says Neil. "Genuine Londoners are down to earth, normal people. We're all the same people aren't we? I think the internet has democratised us all. Made us all more similar."
He pauses, and adds: "I don't know what my point was."
"I find outdoor shops the most interesting clothes shops," says Neil. "There's still that element of feeling like I'm not supposed to be there. When I used to go to the Barbour shop in Manchester there were these old ladies in pearls reaching for the panic button like, 'he's going to rob us!' Cut to three years later and it's an indie disco; all the kids have got skinny jeans, and they're all twenty. I preferred it when I scared the old ladies. You still get that in outdoor shops when people say, 'oh what are you looking for? What's it for?'
"It's like, I don't know? I'm walking around town. I'm not going up K2, mate."
"In Issue 12 we coined the phrase Hikerdelic or Hikerdelia," says Mark. "It's a play on the word hiker and psychedelic. We sat on the idea for a while, and didn't do much with it. But we've started to believe it can be it's own brand. We've done t-shirts, and we've sampled a jacket. A couple of mugs as well. The plan is to grow that into something bigger."
"It's inspired by ticket touts and drug dealers wearing Gore-Tex jackets in Manchester, combined with vintage hiking gear," says Neil. "The Salford ticket tout is definitely up there in terms of style gods. Because they've got a lot of money, they're a bit flash — a bit criminal as well, but always the best dressed."
"And the best connected," says Mark. "Probably travelled around a bit as well."
"They'll be stood outside the Apollo in Manchester with a coat worth a grand and a cashmere sweater," says Neil. "So them and drug dealers; it's pretty close."
Originally published in The City Talking: Fashion, Vol. 2
Written by: Jennifer Lee O'Brien
Photographs by: Shang-Ting Peng


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Apple's CEO Needs to Eat His Words
Tim Cook of Apple is one of the most vocal opponents of the religious freedom bill that Indiana initially passed. And I can't believe anyone let him get away with it. The media asked "tough" questions of Governor Pence, but it let Mr. Cook go free. Well, it's time someone should core the apple and expose the rotten seeds of hypocrisy that lie within
In case you have been in a coma the last few days and didn't hear about it, Indiana passed a bill protecting religious freedom. As expected, the press and folks like Mr. Cook have mischaracterized it. And as is typical of too many conservatives, they have allowed the press to frame the issue. They have been unwilling to recast correctly the questions that have been thrown at them.
The issue isn't whether one person should be allowed to refuse services to someone based on his or her religious beliefs, but whether the strong arm of the government should be used to force people to act contrary to their religious beliefs without the government at least showing a judge and jury some compelling reason to do so.
In a nation that increasingly distrusts government and wants less government interference in our lives, I bet the overwhelming answer to that question would be "Heck no. The government better give me a compelling reason for why it has to trample over my liberty and tell me one more thing I have to do or one more thing I can't do!"
But Mr. Cook apparently wants one private citizen to be able to go into court and have the court order the government to force another private citizen (or the company he or she owns) to violate his or her beliefs without any compelling reasons to do so if someone wants to use that person's services.
If that's what he wants, then he should get what he wants, and the folks at the Manhattan Declaration should help him. The Manhattan Declaration organization should sue Apple for all it is worth because in 2010 Mr. Cook's company declined to provide services to that organization.
And why? Because Apple didn't like the message the organization conveyed.
That's right. Because the Manhattan Declaration supports marriage, life, and religious liberty, Apple removed its app from the Apple i-Tunes App Store. Apple "refused" its services based on its belief system, whatever you may want to call it.
Tim Cook's conscience was apparently violated by what the Manhattan Declaration organization wanted to advocate, so he told them "Go away."
But when a T-shirt maker doesn't want to print a T-shirt with a message he or she doesn't agree with, Tim Cook would say, "Tough! That's discrimination. Government, go make him print that T-shirt."
Mr. Cook may be a technological wiz. He may be a marketing guru. But there is one thing he for sure is—a brazen hypocrite.
I know Christians are supposed to turn the other cheek, but I also know that the Apostle Paul asserted his rights under Roman law when the law was violated.
And Mr. Cook violated the existing civil rights laws that prohibit discrimination—refusing services—based on a potential customer's religion and creed. And religion and creed are clearly at the core of the Manhattan Declaration's message.
Ironically, since Mr. Cook isn't apparently a very religious person, a religious freedom protection law like Indiana's wouldn't give him any protection or defense to such a legal action.
I, for one, think it might be a good time for Mr. Cook's Apple to enjoy the fruit of the position he is advocating and see how it tastes. He may find it rotten to the core.
David Fowler served in the Tennessee state Senate for 12 years before joining FACT as President in 2006. Read David's complete bio.
Get David Fowler's Blog as a feed.


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I took a walk down Newbury Street this morning and I noticed that there were 15 vacant retail spaces from Arlington to Mass Ave. I counted retail space as; street level, below street level, and parlor level. Does this vacancy rate seem high to you?
The good news was of the 15 vacant retail spaces 6 had Building Permits on the window. I hope is an indication that new tenants will be moving-in.
The other item that I noticed, there's no shortage of real estate offices on Newbury Street.


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Chloe Vevrier » German Beauty Roses
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