text: Modwest Blog tag:typepad.com,2003:weblog-1244376 2009-05-15T11:12:13-06:00 TypePad PHP upgraded... and everything else tag:typepad.com,2003:post-66832741 2009-05-15T11:12:13-06:00 2009-05-15T11:18:07-06:00 We have known for quite a while that we needed to upgrade PHP. In fact, it was a top requested answer to the question "What else can Modwest do to help our clients succeed?". We're listening! The answer is is... John Masterson When You Need a Boost tag:typepad.com,2003:post-64047037 2009-03-13T09:18:39-06:00 2009-03-13T09:26:07-06:00 A common challenge in running shared hosting servers is how to ensure everyone gets a fair portion of the shared resources, and prevent any one misbehaving or resource-intensive script from affecting the performance and stability of the server for everyone... John Masterson Feedback Community Launched! tag:typepad.com,2003:post-61121002 2009-01-09T13:00:44-07:00 2009-01-09T13:00:44-07:00 Since our open beta announced in October, we've had some really helpful input by a handful of Feedback Community members, fixed a few things, and improved a few things. So it's time to draw back the curtain and invite all... John Masterson Trials and Travails of Email Filtering tag:typepad.com,2003:post-33024688 2007-04-18T15:00:47-06:00 2007-04-18T15:00:47-06:00 Not counting our managed servers or VPS plans, Modwest handles close to a million messages a day. And mailbox owners (including me!) reasonably want 2 things: Keep junk and malware out of my inbox. Give me all my legitimate mail.... John Masterson


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Originally seen on kingsroad.co.uk – posted on 5 October. Written by Rachael Grace.
"WHERE IS GABILICIOUS?!!?1111" would have been my first line if I were reviewing the series. But the excerpt below is muuuuuch better.
Here's an excerpt:
"BABE, YOU'RE ATTITUDING AT ME!" Yes, we were back in Chelsea this week, where you could say normality was resumed.
Read the rest on kingsroad.co.uk.

subset: commoncrawl


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Every brand has a visual identify—but some make a stronger impression and are more memorable than others. In this post we'll explore the elements that make up your brand's visual identity, and how you can promote it.
Small businesses and startups often find themselves facing a complicated challenge when the work required for growth outstrips the personnel on hand to handle it. Here's how your business can improve productivity without hiring more employees – or overworking the ones you have.
Every small business wants more customers, but deciding how to invest your limited marketing dollars can be difficult. Here are four simple, affordable ways to market your business without breaking the bank.
From ownership to management, every business owner wants to staying abreast of emerging technology and stay competitive. Here's a glimpse into what successful technology implementation looks like in practice, and how businesses just like yours are leveraging today's innovations to their full benefit.
Replacing an employee is a headache no manager wants. That's why we've gathered six simple to follow tips that can help you reduce employee turnover and build a strong – and lasting – office team.
These are the kind of problems we at Xerox have dedicated ourselves to – the kind...
Here's where you will find the next breakout technologies for Xerox, as well as y...
Silicon Valley charter researcher: Long before an office was a place, humans and technology have co-evolved.
Print remains an essential means of communication in the business world where print is still a critical component of virtually every marketing campaign, workflow, and customer interaction. It can also provide the competitive edge you've been looking for. Here's how.


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Always very helpful and willing to take in as many rescue babies as they can! Have seen tons of happy animals go to great homes through DUR. Truly a beautiful organization
Dogs Unlimited Rescue is amazing!! I can't say enough great things about them. They have been nothing but kind to not only the animals but the volunteers as well. Will always be my favorite rescue!!
After my husband and I lost our beloved dog, my sister found the cutest lil' pup, LeBron, online from Dogs Unlimited Rescue and I knew we had to have him. Mary Lou, LeBron's foster mom, was wonderful to work with and though the process was thorough (which is great to make sure each pet is going to a good home) everything went smoothly. Dogs Unlimited Rescue was upfront with LeBron's history and Mary Lou made sure all available records were given to us. I am very thankful for Dogs Unlimited Rescue and would recommend them to anyone looking to rescue.
I have now worked with Dogs Unlimited Rescue on two occasions and can't say enough great things about this organization and their desire to help all animals.
I first adopted a Chihuahua mix that had been found as a stray on the streets of Cleveland in October 2015 , the poor little guy ( now known as Charlie , formerly Niko ) was in pretty poor shape , he was very much underweight and had apparently been attacked by an another animal of some type , which had caused head and neck trauma , as well as a raging infection throughout his tiny body , as these injuries were not recent.
Dogs Unlimited took this boy into their care , and after putting drains into his wounds , stitching him up and treating with antibiotics , he was on his way to a full recovery. I fostered him for a while and ended up taking him into my family, where he is very much loved !
My second occasion to work with this wonderful group of people was in January of 2017 , I had heard of a senior boxer that had been abandoned by her family at a facility , which had apparently been trying to find him a home , although their hearts may have been in the right place , their actions proved not to be , Rosie was put in an unheated outdoor building and was kept there for approximately a month and a half , they had not found her a home and were going to be euthanizing her the next day.
After I saw a picture of her on Facebook with a plea for someone to step in , I went up there , and what I found was heartbreaking , it was 6 degrees outside , she was in a small pen with no bed on a concrete floor, lying in her own urine. She did not even lift her head to look at us , just opened her eyes and then closed them again. I called Dogs Unlimited , hoping they could help me out with this poor girl , they immediately said yes , for which I was so grateful ! So I got her out of there , it took her 10 minutes to walk 20 yards to my car , her arthritic body was so sore from the concrete floor and bitter cold temperatures she had been in for 1 1/2 months. I took her home , warmed her up , fed her and made lots of comfortable beds around my house . After giving her a day to settle a little , we took her to the vet , she had some health issues , thyroid condition , tumors , deafness , partial blindness and unfortunately she has been diagnosed with Cancer.
After being in our care for a few weeks , the spark came back to her , she is now one very happy girl , who loves her new home and her new furry siblings . We are committed to making the time she has left comfortable and pain free, with lots of Love !
None of this would have been possible without Dogs Unlimited Rescue, they truly care about the welfare of the animals and always have their best interest in mind , they always go the extra mile for these rescues , as difficult as it may be at times .
Christine Fronczek
I have volunteered with Dogs Unlimited Rescue for four years and I can't imagine volunteering anywhere else. I am constantly amazed by just how much the people at Dogs Unlimited Rescue care about every single dog that comes into rescue. It doesn't matter if they are old, young, fit, disabled, large or small; Dogs Unlimited Rescue spares no expense in ensuring that each dog gets whatever they need to give them a second chance for a happy life. I have grown attached to a lot of the dogs in the rescue, especially the ones who were in the rescue for a while while they waited to find their perfect home, but I remember one of the dogs who was there from my very first adoption event. His name was Leon and he was a little black and white papillon who was terrified of men which meant that I couldn't adopt him because of my dad and brother. I think that Leon was adopted and returned two times and every time Dogs Unlimited Rescue welcomed him back into the family which shows that they are willing to do everything they can for all their dogs, no matter how long it takes. And while I still miss his perky little ears and unique personality, I know that he is in a good home now since Dogs Unlimited Rescue carefully selects each adopter to ensure that our dogs will be treated with the love and respect that they deserve. I don't know that I can capture with words just how much Dogs Unlimited Rescue cares for their dogs. They tailor their care to each dogs individual needs and do whatever it takes to make sure that each dog has the best life they can have. I think that the best compliment I can give is that if I were a dog, there would be no one that I would trust more to help give me a second chance.
This is our first dog adoption. We couldn't thank Dogs Unlimited Rescue enough. They made the whole process easy. We were given time to get to know our dog and they were very patient with our concerns and questions. The volunteer staff were exceptionally kind and helpful. They even brought toys and some essentials to our home to help with the transition. They made sure that there was a perfect match between a dog and his/her new home. We would highly recommend Dogs Unlimited Rescue to anyone who is looking to adopt a dog.
Dogs Unlimited Rescue is a wonderful organization full of amazing volunteers and foster parents! Their goal is to provide a better life for all animals they rescue. This is truly an amazing organization, and I cannot begin to express what an asset they are to the community!
Dogs Unlimited Rescue is an exceptional rescue for many reasons. I am writing this review from the perspective of a foster volunteer but I also adopted my sweet, beautiful Ruby from DUR. Our Director, Gina, is an amazing, compassionate individual who always goes above and beyond for the rescue animals in our care. We know she will find a way to provide the care needed, no matter the cost, to restore our babies back to health. Many of these dogs (cats and a pig too!) require costly vet care into the thousands for their recovery and management of complications. As a volunteer, I always feel supported and respected and I'm proud to be associated with such a wonderful, caring group of volunteers. My current foster, Allie, was close to death when found due to blood loss and hypothermia after apparently being hit by a car and left by the side of the highway. Fortunately she had an angel who found her and made it possible for us to take her into rescue and provide her with the emergency care she desperately needed. After an amputation and management of complications she's recovered and is now in search of her forever home. This is just one of many stories that illustrated the amazing work of this rescue and its volunteers! I'm attaching a photos of my dear Ruby and current foster Allie (wearing the pink sweater).
My husband and I have been volunteering / fostering with various dog rescues over the past 17 years and NOT all rescues are equal to Dogs Unlimited. All rescue work is very worthwhile and everyone has good intentions but rescue is not an easy business and requires a lot of dedication, we feel very fortunate to be a part of Dogs Unlimited because they are STARS in our eyes. I work in the medical field and so for me the healthcare aspect of each and every dog is a "very important" aspect of rescue. I could not in good conscience continue to volunteer for DUR if they did not also share that vision. It is not just a matter of rehoming each dog but caring for them both physically and emotionally in preparation for their forever homes. Yes sometimes the cost of doing so can be tremendous and sometimes a great deal of time is required but that is where this rescue shines, everyone pulls together to make it happen. We never feel rushed to move the dog out before they are really ready, each dog is different and they are given however much time they need to heal and feel safe. I feel honored to be a part of a rescue that I respect as much as I do Dogs Unlimited and its founder Gina Lutes.
We adopted our special needs foster Kaden who clearly came from puppy mill situation and was thought to be a piece of trash on the street until someone attempted to pick him up. He came to us with a terrible fear of human touch, he didn't know what grass was and his first experience with sunlight amazed him, he would raise his little face the light and squint. Due to his previous living conditions he continued to develop health issues, he went blind from his diabetes, required daily insulin injections and had much hearing loss from untreated ear infections. BUT Kaden became a happy active dog and lived out his last few years thoroughly enjoying human cuddle time and exploring the outdoors in a comfortable controlled setting, he became a spunky little boy once he knew what it was like to be truly loved and to feel safe. RIP sweet Kaden. Thank you Dogs Unlimited!
I received a call from Dogs Unlimited Rescue a few years ago asking if I could foster a pair of Pekingese dogs that were being rescued along with a slew of others dogs. I was happy to help!! I ended up adopting them because I fell in love!! The people at Dogs Unlimited Rescue do everything possible to help unwanted and abused animals find "furever" homes. The 100% commitment the director, staff and all the people involved with fostering and finding these animals homes show what true animal lovers they are. I am truly grateful I received that call and for all their efforts!!
Dogs Unlimited Rescue is absolutely amazing! I learned of their efforts through a co-workers and have been following them ever since. I have a wonderful 6 lbs toy poodle at home who was very shy and there was a small dog in the rescue that we thought might be a good match/friend for my dog and household. Sadly this was not the right one but it got me to the point of filling out the application piece. They got to know me and understand my abilities with the smaller animals and not more than a couple weeks later came my little Tucker. He fit right in. They took care of everything at the Vet's I had nothing to worry about. He was a very scared little guy in a new place, but Tucker and I bonded very quickly. He didn't know how to play like other dogs, he didn't understand fetching, and with my other toy poodle who can do tricks galore he has learned so much. He even tries to play with the cats by pushing the toys in their face, it's absolutely hysterical (not from the cats' point of view). I wouldn't know what to do without him now, my little white thunder! I couldn't ask for the rescue to do anything more than they did, they are the best at creating new families.
Kathi M.
I am composing this letter for the best animal rescue in my opinion and I'm sure many others would agree with me, "Dogs Unlimited".
Two years ago a dear friend of mine asked me to foster a dog who was found wondering the streets on the west side of town here in Cleveland. My friend volunteers for "Dogs Unlimited" and I was quick to say yes as I totally trust her judgement when it comes to dog rescues. I was involved a few years before with another rescue of which I adopted two dogs, one of which I fostered and eventually kept. My friend then started to volunteer for Dogs Unlimited and I am so grateful that she did and asked me to get involved.
The dog she asked me to foster was a Min-Pin. Now, in all honesty, I am a "big dog" lover, so this was going to be something new for me to foster a " small dog". Dogs Unlimited brought the dog to me and immediately I knew I was volunteering with a very special rescue. They stayed with me while we, Diesel and myself, became acquainted. They told me which food was best for this little guy, his vet appointments were made by them for me to take him to for his neutering, shots and just general up keep. They were not in a hurry to just dump this dog, they wanted to make sure he would be alright with me and that I was alright with him. This was so important. If I had a problem I had a number to call and they would get right back to me. They explained the process of me taking him to meet prospective adopters.
Long story short Diesel is now my third pup, just could not let this wonderful little stinker go. He is a huge part of our lives and what is great is Dogs Unlimited stays in touch with us. They are there for us 24/7 and that is what makes them so special to me. They give so much time and support always and Gina Lutes is awesome. If you have questions you get prompt answers, if your dog should happen to wander off, they are out looking for him or her right along side of you. This dog rescue is way above and beyond other rescue organizations.
There is no rescue too challenging or level of care too great for Dogs Unlimited! When there is a need, Gina Lutes and her unbelievable support team jumps right in and changes the lives of both fur babies and their future owners forever! As a volunteer, I have seen this over and over again. I am proud to be associated with such an unbelievable organization and I applaud their hard work and dedication!
dogs Unlimited rescue has made a profound impact on our community and my life personally. They have saved so many helpless dogs and have even recently saved a helpless pig. The owner and her fosters and the board work endless hours to care, nurture and love these animals and bring them their fur ever families. I am so blessed to witness these beautiful animals being rescued and it has made the biggest difference to these hopeless animals that end up having a happy ending because of this organization I rate this organization beyond a 5 star! --Sharon Witmer, Lucky family member of rescued dogs!
Dogs Unlimited Rescue is a safe heaven for rescued animals! This organization puts 100 percent effort and commitment to provide a new life to their rescues. A dedicated team of volunteers work tirelessly to provide the animals unconditional love, safety , good health and happy homes. This organization has an examplary reputation and is truly an invaluable resource to animal rescue community.
My name is Terri and I'm Buddy's mama as of June 2016, thanks to the wonderful caring people at Dog's Unlimited Rescue. Buddy was a street runner who was so fearful of people. The Rescue went the extra mile to make sure he felt safe and secure, and also got him a chip and gps. Mary Lou kept in close contact with me until she felt Buddy was ready for adoption. And we've continued to commune through all his wonderful progress. I'm so grateful for Dog's Unlimited Rescue. Thank you and God bless you all.
We heard about Dogs Unlimited Rescue through PetFinder. We looked at so many dogs for weeks. (We wanted them all)!
Finally, we clicked on "Lucky's" page and we knew he was already part of our family. His pictures showed how loving, handsome and proud he was. When we talked to Mary Lou Bender, Lucky's rescuer, and heard his story, we couldn't wait to meet him!
Lucky met our other rescue Baxter (pug), and the bonded immediately! I felt Lucky felt right at home as he walked around our house, played in the backyard, and found "his" spot on the couch! We love Lucky, aka, Lucky Ducky Doo. He and Baxter are best friends and enjoy their couch time and walks together! Lucky keeps adjusting more every day and I think he now knows this is his forever home and we are his forever humans!
Mary Lou and Dogs Unlimited guided us through the whole process, had all of Lucky's medical records in order, and followed up with us regularly after Lucky came home, to make sure not only Lucky was happy but Katy, myself and Baxter were all happy with Lucky too!
We will always rescue and own through Dogs Unlimited Rescue! We highly recommend them to all current and new dog owners!
Greg K. & Katy B, and Mr. Baxter too!
I've fostered for dogs unlimited many time the rescue is a wonderful organization. They have the best vets taking care of the animals to get them ready for there forever home. They get dogs that no other organizations would take with broken limb and many expensive problems etc.while fostering I fell in love with my little cockapoo he was only 2lbs and very sick with lots of care he is now a healthy baby boy. Dogs unlimited rescue.org is a top notch rescue group they have given many dogs new lives with there new forever homes
DUR is an amazing organization. I know first hand after going through the adoption process. These folks are true animal lovers and everything they do is with the best interest of the dogs in mind. They fund raise to provide life saving care to dogs who would otherwise suffer. My own pup was the beneficiary of cardiac surgery, funded from the generosity of people who believe in DUR. Two years after that surgery, he is in great shape. He is the happiest dog I've ever met and loves to run. Without DUR's commitment, fantastic fundraising, fostering, and coordination of care, his heart condition would have been fatal. I have remained in communication with these great folks and enjoy watching them rally around the dogs who are lucky enough to find their way to DUR. Not only are the dogs well cared for, DUR does a wonderful job of finding the right environment for these dogs, many of whom have suffered trauma we will never know. I also appreciated that this organization is run in a professional manner, with business savvy. There are many dog rescue groups that mean well but don't have the business know-how to operate. DUR is run with compassion for the dogs and business sophistication. (I don't mean to leave out the occasional cat or pig that finds its way to DUR--they are in great hands too!)
Previous Stories
I adopted a dog from this wonderful group. His story is remarkable. He was surrendered by his owner after she was in a life threatening accident. At his first checkup, the vet discovered a heart defect that would cost thousands of dollars to correct. With no notice, this group mobilized, collected enough for his surgery, and saved his life. They were thoughtful in finding him a home after the surgery, taking into account the many intangibles that make the difference between a great fit and a problem. His foster family was incredible. I've never seen such an amazing and dedicated group of people.
The care and the concern of this organization is extraordinary. There are no conditions too dire or distance too far that would stop this group of volunteers from rescuing these dogs, or any animal, from dangerous or uncertain circumstances. And very importantly, they are very good stewards of every dollar. Everyone is a volunteer and everyone is committed to Dogs Unlimited Rescue.


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"Aim", "shoot" and "fire"; the language of the gun is shared with that of photography and film. It's not surprising that the camera and gun evolved together. In the 1880's cameras with sequential frames for time and motion studies were made from modified guns with a rotating disc of light sensitive film recording in the place of the bullet that would have frozen motion. Sarah Pickering
Locus+, in partnership with the NEPN and Durham Art Gallery are proud to present Aim and Fire, the first solo exhibition of work by Sarah Pickering in the North East. The exhibition includes works from Explosion, Incident and Fire Scene as well as Celestial Objects, a new commission by Locus+, in partnership with NEPN.
Celestial Objects has been made by photographing a revolver fired in total darkness. Each photographic exposure captures the entirety of a gunshot from start to finish.
The gap between truth and what we understand to be truth is a fascination that runs through Pickering's practice. Celestial Objects continues this investigation, referencing images of deep space, cinematic special effects and the skies of Romantic painters such as John Martin, positioning itself between the objective facts of a documentary photograph and imagined reality.
Exhibition produced by Sarah Pickering in collaboration with Locus+ and Durham Art Gallery.
Exhibition Preview: 17 October 2013, 6.00-8.00pm.
Artist Talk in partnership with Contemporary Arts Society: Saturday 9 November 2013, 11.00am-1.00pm. Free (with entry to exhibition) booking essential: firstname.lastname@example.com
Associated event: Portfolio Reviews with Sarah Pickering: Saturday 9 November 2013 at The NewBridge Project, more information HERE.
Opening Times:
17 October – 12 January 2014.
Open daily 10.00-5.00pm in October; 10.00- 4.00pm in November -January.
Free (with entry to exhibition) booking essential: firstname.lastname@example.com
Durham Art Gallery, Aykley Heads, Durham, DH1 5TU.
View the map HERE
Image credit:
Sarah Pickering, 'Muzzle Flash' from Celestial Objects.
In their new project Deleting Memory commissioned for The Social: encountering photography, Indonesian-based artists Irwan Ahmett and Tita Salina set out to explore the relationship between the city and its […]
Extending his exploration of the English social landscape, Simon Roberts has worked on a major commission for The Social: encountering photography. A series of large-scale colour photographs, made in various […]
'Ships that Pass', a new photographic installation for the windows of the National Glass Centre references the River Wear and the sense of purpose it achieved in over 600 years […]
© Copyright 2019.


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Jemma Bradwell, University of Wollongong
Katina Michael, University of Wollongong
Wearable cameras and portable technologies might seem like creations reserved for the likes of James Bond or Jason Bourne. Such devices, however, were the focus of the recent Sixth Workshop on the Social Implications of National Security, which examined the use of camera technologies by and against law enforcement. The event, convened by Associate Professor Katina Michael and held at the University of Sydney, was entitled 'Sousveillance and Point of View Technologies in Law Enforcement'.
Suggested Citation
Jemma Bradwell and Katina Michael. "Security workshop brings 'sousveillance' under the microscope" University of Wollongong: Latest News Mar. 2012.


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Spring Break! Wooooooo hoooooooo…. right? Depending on where you are in life, you might not be quite this excited about it.
If you're like most of us in the Lionz Den, you've moved beyond the crazy fun of your college days into a more… uh.. sedate way of life. (I stopped myself before I said it was "boring" because frankly I would give anything to be bored)
Because apparently we like to torture ourselves, we compiled this chart listing the differences in Spring Break then and Spring Break now. If you find you're still in the Then column, enjoy the hell out of yourself before Spring Break turns into Spring BROKE.
If you've fallen into the Now column already then stay strong, my friends, and be proud of yourselves for making it out alive. Cheers!
Thanks to our friends Jason, Lisa, Jeff, Rob, Philip and Eric for the assist. Kudos to Brett for always making us look like we have mad Photoshop skillz. (credit for blog title to urbandictionary.com)
Copyright © 2012 Little White Lion
All Rights Reserved


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Disruptive Behavior Disorder
pusha.se - 1 aug 2016 av adjustmentdis i disruptive behavior, nos
Disruptive Behavior Disorder NOS The child has symptoms of these two illnesses, but not enough to actually be considered impacted by the disorder completely, or perhaps there isn't enough info about the child's situation. Symptoms of Disruptive Behavior Disorder:
Consistent defiance of authoritative figures Inability to take responsibility for poor behaviour Temper tantrums on a regular basis Vengeful behavior and bitterness Aggressiveness toward animals or people Ruining property Bullying Endless rule breaking
Reasons for Disruptive Behavior Disorder NOS Reasons for Disruptive Behavior Disorder NOS can be both environmental and biological. What this means is that it can run in the child's family (possibly one or both parents displayed the same behaviors as kids) or it can be a result of trying and unhealthy environments that the kid is exposed to. Some children have ADHD that leads to their behaviour disorder or neurological disorders. If a kid was exposed to maltreatment at a young age, abandoned by a parent, not cared for properly (i.e.,being malnourished or the family is in poverty), all of these variables can cause Disruptive Behavior Disorder NOS. So, it's vital that you remember that all of these reasons are outside the child's control, and the kid shouldn't be viewed as awful. All kids influenced by conduct illnesses deserve and need treatment to get better.


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How Bright Light Therapy Helps with Low Mood, Sleep Problems and Jet Lag
By Daniel F. Kripke, M.D.
Last Revised April 2013
About Dr. Kripke
Daniel F. Kripke, M.D. is a licensed physician certified by the American Board of Psychiatry and Neurology and an emeritus professor of psychiatry at the University of California, San Diego. Dr. Kripke was elected a fellow of the American Psychiatric Association. Dr. Kripke is also associated with the Scripps Clinic Viterbi Family Sleep Center, La Jolla, California. Dr. Kripke has co-authored hundreds of medical articles and has given invited lectures in 18 countries. In 1973, Dr. Kripke established one of the first sleep clinics in the United States. In 1981, Dr. Kripke published the first controlled clinical trial of bright light treatment. For the past 30 years, Dr. Kripke has been doing research on light treatment and treating patients with light.
Dr. Kripke has now retired from practice and no longer consults with patients. Please do not contact Dr. Kripke for personal medical advice. A physician should not give personal advice to a patient he does not know, and the California Medical Board considers it unethical to provide mail or email advice to people who have not been examined. Treatment of depression is best obtained from a nearby psychiatrist. Patients with delayed sleep phase disorder or advanced sleep phase disorder might find help at a sleep center such as the Scripps Clinic Viterbi Family Sleep Center in La Jolla, California.[67]
If you have a sleep problem, you might also be interested in reading Dr. Kripke's other ebook, The Dark Side of Sleeping Pills (in Amazon Kindle format), also available at the website. Some of this material is repeated in both books.
Acknowledgment: I have been fortunate that my research has been supported for over 35 years mainly by the U.S. National Institutes of Health (NIH) and the Department of Veterans Affairs. In the past, agencies of the U.S. Army and Navy supported work of Dr. Kripke's laboratory, and for two years he did medical research in the U.S. Air Force. The Department of Psychiatry, the Sam and Rose Stein Institute for Research on Aging, and the Center for Chronobiology of the University of California, San Diego have also supported my research, as has the Weingart Foundation and the American Cancer Society. Recently, the Scripps Clinic Viterbi Family Sleep Center has been supporting some of my research, particularly the most recent study of mortality associated with hypnotics, assisted by the help of generous gifts from private donors. Ambulatory Monitoring, Inc. (the manufacturer of the Actillume and other actigraphs) and Minimitter-Respironics (makers of actigraphs, now part of Phillips), The Sunbox Company, Apollo Health (now part of Phillips), and Enviro-Med (which make bright light boxes) have supported our research, partly through joint research projects funded by the National Institutes of Health. It is important that readers understand for whom an author has worked. Dr. Kripke has no financial interest in any of the companies or products mentioned or in any commercial web site, apart from a small percentage of the tiny price of his eBook. Being supported largely by public funds, I am able to speak out for public interests. I appreciate this opportunity.
Endnote for Chapter 10
67. More information about Scripps Clinic Viterbi Family Sleep Center in La Jolla, California, is available online at www.scripps.org, or by phoning (858) 554-8845. [return]
Table of Contents
Brighten Your Life, in all its formats, including this eBook, copyright ©1997-2018 by Daniel F. Kripke, M.D. All rights reserved.


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For about a hundred lucky middle school students this summer, the University of Alaska campus becomes their home as they solve unique engineering problems at the annual Alaska Native Science and Engineering Program Science Camp.
At this time of year, the Anchorage campus is quiet. The kids can be found among the largely deserted buildings, past empty parking lots, past the looming Conoco Phillips Science Building, and around the corner. They're playing ball, taking a break from more than a week of concentrated work, solving unique problems, and crafting engineering solutions using common materials in unusual ways.
Most of the work takes place inside the ANSEP building, and it is rigorous and fun at the same time. All the middle school students chose to be here. Those from the Yukon-Kuskokwim Delta came for the same reason, and it has little to do with engineering.
"I just came here to have something to do," said one student.
"I wanted to come because I didn't want to stay in St. Mary's, like, every single day, cause it's a small village and all I do is just walk," said another student.
"I can actually do something instead of laying around at home being bored," added one more.
Audrey Alstrom is the Regional Director of the Alaska Native Science and Engineering Program Middle School Academy, and the director of the Science Camp.
She describes a day that would make anyone tired. It involves academic work and solving difficult problems, things they won't find in a book. Yet these kids do it with intensity. No one has to tell them to get to work.
"They're here 24 hours a day. They're busy doing academic activities. Then they have lights out at 9:30," Alstrom said. "Then up and at it again the next morning at 6:00."
One week into camp, students have already done a lot, but the favorite of most is the time spent building a computer. They plugged parts into a motherboard and it took some care.
"If we shocked it or messed it up, our computer wouldn't work," Ayva Matchian from Chevak explained. "We grounded ourselves. We put our foot on the ground and touched metal for how many seconds, and then touch it, and then put it in the computer."
For most, it wasn't all that easy to build computers that worked, but Adryan Green from Bethel says that he had a head start on that project.
"I just know how to. I've seen, like, YouTube videos," he said.
Students at Science Camp not only do the work of creating unique engineering solutions, but have to reflect on what worked and did not work in the process. While others had moved on to other projects, Sarah Bowker labored over a keyboard typing in her evaluation. She wrote that she loved making a computer, but shared some self-criticism:
"One thing that didn't work for me was how slow it went. I'm really impatient."
"You've all been using the engineering process," Alstrom said, standing in front of the class. "You just didn't know it."
The engineering process, as she explains it, requires that each student understand the problem they're solving and its parameters, like materials and time frame. For example, they had to design a Mars lander that could handle the shock of landing, while keeping the astronaut safe. In this case, the astronaut was a marble and the materials: paper cup, straws, duct tape, bubble wrap, and a pack of cards.
The next step was going online to do research before coming up with a design. Then the teams made and tested their designs. Laurita Nerby from St. Mary's says her team didn't do so well. They lost their marble astronaut.
"Yep. Just tipped over."
Adrian's team did fairly well, bringing their Mars lander near the target with all on board. But it was Ayva's team that took the engineering process that all-important extra step. When their initial design did not perform well, they went back and made it better, starting with re-designing the parachute.
"The first time we used pipe-cleaners, which were bendable and a bad idea." Says Ayva, "So we went with the straws because they were stiffer."
It worked.
"Yeah, I'm starting to like this engineering thing."
It's too early to know if an engineer was born at the ANSEP Science Camp this summer. That will take years to discover. Meanwhile students have moved on to their next challenge: designing robotic fish. Those instructions may be a little harder to find on YouTube.


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Energy in Sericulture
sericulture industry is an extremely labour intensive cottage industry employing
about two million people in the private sector and twelve thousand five
hundred people in the public sector in Karnataka. The Government of Karnataka
operates seven filatures (wherein cocoons are reeled for obtaining silk
yarn) besides silk mills and several rearing farms (where silk worms are
reared). The area under mulberry cultivation is growing rapidly and is now
more than 265 lakh acres in Karnataka. Of these, 98% of mulberry gardens
are in the districts of Mysore, Mandya, Tumkur, Bangalore and Kolar.
The government silk filatures and silk factories derive their stream and hot water from coal-fired boilers of antiquated design. Although the component of the energy cost in the total cost of silk produced in such filatures is rather modest, the substitution of the present fossil energy resource (coat) by solar energy has several decided advantages like :
It is in this context that KSCST has initiated a project with the objectives of investigating the scope with the objectives of Investigating the scope of solar energy applications in sericulture and demonstrating them on a pilot basis. Specifically, use of solar energy for (1) supplying hot water for reeling and (2) cooling and/or heating of buildings for rearing silk worms and for storing cocoons are being explored.


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by Alan Bock
If the dominant themes that have been used to justify the Iraq war recently were sincerely held, the administration and the neocons should be doing backflips of joy this week. Building an independent and sovereign Iraq, preferably with a government responsive to the people? Check. When the Iraqis stand up we will stand down? They're standing up. You could even make an argument that the "surge" has helped to induce the kind of stability that should strengthen the political structures to the point that Iraq's leaders feel more secure and self-confident, which should allow them to act on behalf of the whole country rather than for strictly sectarian goals.
Apparently, however, this isn't quite the kind of Iraqi democracy the Bush administration had in mind.
To read the rest of this story click here.


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Experience Breakthrough Learning!
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The Subliminal Learning System
We have developed a proven system that will improve your performance abilities dramatically, in the fastest possible time! All that you need to do to make it work is listen to our audio cassette program, and within just a few short weeks you will experience an amazing improvement which is unmatched by any other method. You will also find that using our system will help you to get muchmore out of any other study method, books, or lessons that you are currently using! Best of all, subliminal learning requires no effort on your part, other than passive listening to our audiocassette! Nothing for you to pay close attention to, nothing to memorize! The system is totally automatic!! Yes, we know that this sounds too good to be true. But we assure you, IT IS TRUE that for most people who try it, this system really works!
Our Subliminal Learning Systemhas been helping thousands of people all over the world achieve their true potential! That's not some slick advertising line, it is the honest truth. We have received many testimonials from around the world! All letters are on file at our office, and are available for inspection by any authorities that are concerned with our claims!
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Using our programs is extremely easy! The program only needs to be played at a very low volume level, so that it is just audible when your attention is directed to it. Then you're free to go about doing whatever you like, within listening range. Watch TV, listen to music, exercise, meditate, play poker, etc... You can even listen while driving your car. No effort required, absorption is automatic. All of our programs are 100% safe, producing absolutely no side effects! Complete message lists are included, so that you will be fully aware of what is being programmed into your subconscious.
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Music and Performing Arts Programs:
All of our music and performing arts programs are designed to be useful to individuals of all experience levels. From novice to seasoned professional, you will benefit greatly by the powerful effect of our subliminal programs
Guitar & BassMaster the fretboard! You can be a much better player with the right mind set. Don't allow what you might "think" to hold you back. Progress faster, learn more in less time! Develop the style and control that you crave.
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General Interest Programs
Memory Enhancer Stop struggling to remember! This program will help you retain and retrieve the deluge of information that pours into your brain every day. This is our second best selling program, you will love it!
Confidence Need a boost of self-confidence? Well here it is! A strong belief in your own ability is basic to success and satisfaction.
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Relaxation The title says it all! We all need it. It seems that relatively few really know how, and even fewer actually do it. Get to that warm fuzzy place you need to be sometimes.
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Contact Information:
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How Does It Work?
The subliminal learning technique, also called subliminal motivation or subliminal stimulus, is a method of influencing the subconscious. It is probable that all of our senses participate in the ongoing development of our subconscious mind. However, the senses of sight and hearing are our best available avenues for connecting with our subconscious mind. We have found that the sense of hearing is the most convenient to use method of producing an effective subliminal programming tool.
Almost everyone can benefit from the advantage of reducing the pressure of negative or erroneous subconscious thinking. Anyone who has ever said, "I'm hopeless at this" or "I could never do that" most certainly could benefit from this system. Prejudging your abilities is a sure sign of negative programming. Like the thousands of people who are using our system, you will find that it can make a positive difference in your life.
The key to subliminal learning is a "time compressed" message list. In order for subliminal reprogramming to be effective, thousands and thousands of positive reinforcement and affirmation messages must be delivered directly to the subconscious. We time compress several key messages for each subject using the latest digital technology. All of the unused space (silence) between every word is removed. A computer-controlled technique is then used to speed up the messages several times' normal speed. The computer is able to retain the original tone and clarity of the original voices.
These messages are repeated over and over at various rates, thousands of times per hour. The messages are received and understood only by the subconscious, which learns and uses them to help focus and guide your conscious thought and actions!
We use male and female voices, with the female voice directed mostly to the left channel, in order to reach the right hemisphere of the brain and the male voice directed mostly to the right channel to reach the left hemisphere. The program is highly effective under any listening conditions when used as directed. This technique is simply an extra tool that may help to enhance a subject's success in case of a resistance to reprogramming.
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US vs. THEM
Other subliminal programs on the market place their messages low in the mix, just at the "threshold of hearing" about 60 decibels or more down in the mixed volume. They mask them with the sound of ocean waves or some boring "new age" music that you must listen to over and over again. No matter how close you listen you will probably not hear a trace of their processed messages! Our method uses a special randomizing sound track (it sounds like bubbling water) that the subliminal messages are blended in with. The messages are mixed at a level only about 30 decibels down in volume! If you listen very closely, you can detect the sound of our specially processed messages. The sound is like that of a garbled din of voices. This method provides you with a certain exposure of subliminal messages right up "in your face" for a truly powerful and overwhelming effect!
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You owe it to yourself to give our programs a try. You have absolutely nothing to lose and very, very much to gain. There is a "FAST TRACK" to better performance, and we're on it! Join us! You will not regret doing business with our company. We absolutely assure your complete satisfaction, FOR LIFE!!
If you are not pleased with our product then we do not deserve your money, and you will have every penny of it back, at your request, upon return of the product. We go a step farther than any other company in this business by offering you this total satisfaction guarantee, for life! No time limit!! Try the program for as long as you like, if you find that it's not for you, return it to us for a complete refund!
Remember, we have been in this business, serving people just like you by mail-order for over ten years with no incidence of complaint from the US. Postal Inspector, Better Business Bureau, or the R.I. Attorney General's office.
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A few thoughts about the mind...
We all know that optical illusions can trick us because of the way that we see. Science has now discovered that cognitive illusions, biases deeply embedded in our minds, can trick us because of the way that we think! Our conscious mind can easily be tricked into thinking that it "knows" things that just aren't so!
Quick, if you take off in a helicopter from Los Angeles, California and want to go to Reno, Nevada, in what direction do you head? East, right? Wrong! The answer is North-west. If you head due south from Detroit, Michigan, what is the first foreign country that you'll hit? Mexico, or maybe somewhere in South America? Sorry, the answer is Canada!
Which weighs more? A pound of feathers or a pound of lead? Hey, a pound's a pound but I'm never surprised when 80% of people answer "lead!" If you drop a marble and a bowling ball from a ten story building, which hits the ground first? They both hit at the same time! It seems to defy logic, but it's true that all objects fall at the same rate.
Our conscious minds are filled with these kinds of little misconceptions. This is true even though we have a great deal of direct control over our conscious thoughts. So too is the subconscious mind, mired in misconceived ideas about what we can or cannot do. Always lurking in the background, unnoticed but very influential in its effect upon our day to day lives. Often holding us back with chains of self doubt and fear.
For many of us our subconscious minds are a storehouse of negativity. Programmed by forces outside of our control, the subconscious can become a lead weight upon our potential and possibilities. It is very difficult to achieve a goal if you doubt or fear testing your ability to achieve it. Believe you can't, and most certainly you won't!
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100% lifetime satisfaction. As has been our company policy for over fourteen years, your satisfaction is guaranteed or your money will be fully refunded, for life! No hassles, no questions asked. We can only make this guarantee because of our certainty that you will be extremely satisfied with any and all of our programs. An overwhelming majority of our clients would never part with their programs, even for ten times their money back! We are the only company producing subliminal programs in the world to offer such an impressive assurance of satisfaction for their products.
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Last Revised: 12.27.2003
Last Revised: 12.27.2003


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About the lobes…
The Ideal Marketing Agency for Micro to Medium Sized Business.
LeftBrainRightBrain Marketing delivers big results for micro to medium sized businesses and professional service providers.
Focusing on emerging to medium-sized and home-based businesses, LeftBrainRightBrain Marketing is an independent marketing, branding and advertising agency focused on the needs of smaller businesses. Because of this focus, we understand the unique challenges entrepreneurs face in marketing and growing their business and their brand, without the benefit of large marketing budgets.
LeftBrainRightBrain delivers big-agency results without the big agency egos, hassles and budgets. We're sensitive to the considerations of micro, small, and home-based businesses because we are one.
Our goal is to ensure that your marketing, branding and advertising efforts are logically creative. If they are, you'll connect with the people who make the biggest impact on the success of your business – your clients, prospects and employees – with the right message, at the right time and place, using the right channel and medium. LeftBrainRightBrain solutions are designed to engage, educate, motivate and compel your audience to take the actions you desire. When that happens, you achieve success.
LeftBrainRightBrain offers a full range of marketing and advertising agency services to help you achieve your goals, including:


text:
This month, 3M launched a change in the Incognito lingual braces system which it has been using for years. It did so together with Treatment Management Portal (TMP). This is a platform that allows orthodontists to plan treatments and digitise models. With this step, many procedures become digital, which can increase precision and save a great deal of time.
Incognito Lite Invisible Brackets use the same cutting-edge technology that the original Incognito system uses, that is to say, fully customised and personalised brackets and wires bent by robots with maximum precision. However, Incognito Lite, a "lighter" version, uses fewer brackets, which are completely invisible, and corrects the dental problem with only 3 wires.
I recommend Incognito Lite for those patients who do not require full treatment with lingual braces. For those patients who used to wear appliances and have had a slight relapse, the option of Incognito Lite as partial treatment with invisible lingual braces is very appealing. Only 8 brackets are placed on the front teeth, not throughout the mouth as in the Incognito full treatment. Furthermore, the partial treatment is much shorter than the full treatment.
Para más información consúltanos llamando al teléfono 93 832 72 81.


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Posted on
Last Date October 09, 2017
Punjab Health Initiative Management Company Jobs 2017 PHIMC Lahore
Vacancies / Positions:
- Assistant Manager HR
Age Limit:
- Age should not be more than 35 years as on the last date of receipt of the application.
Salary Package:
- Market-based salary package will be given to the applicants.
The most effective method to Apply for PHIMC Jobs:
- Applicants satisfying the previously mentioned criteria ought to send their applications alongside point by point CV by means of the email address on email@example.com.
- The last date for applying is October 09, 2017.
- The candidates are instructed to compose the name with respect to the connected post in the headline of an email.
- Inadequate or applications got subsequent to shutting date won't be engaged.
- Just shortlisted candidates will be reached for talk with appearance.
- No TA/DA will be qualified for the candidates.
- Along these lines, keep in contact with this page for all parts employments.
Last Date:
The End Date To Apply is ninth October 2017.
Note:If you are unable to see Image Please Refresh
Source: Jang Newspaper (September 23, 2017)
Tags: Punjab Health Initiative Management Company Jobs 2017 PHIMC Lahore for Assistant Manager, Punjab Health Initiative Management Company Jobs 2017


text:
What Is SIO365 Networks?
The Roots Multiclean Product Stable Comprises Of Comprehensive Cleaning Solutions For A Wide Variety Of Industrial, Commercial And Domestic Cleaning RequirementsRoots Range Of Products Are Dry Vacuu
We, Sahara Trading Company Are Well Reputed In Providing Top Of The Line Products To A Rapidly Growing Establishment. We Offer You The Best Quality Products With Utmost Commitment To Meet Your Require
We At Shades Electrical Have Our Own Equipped Factory For Designing And Manufacturing A Wide Range Of Low Voltage Switchgear Power Panels (m.d.b, S.m.d.b, Power Correction Panels & Motor Control Cente


text:
Motorcycle driver impact gel pad seat for harley davidson bad boy 95 97 models.
Very easy to mount on the seat.
Comfortable in any weather.
You can strap it very easily and then remove it when not needed.
Takes the vibration and sore spots off.
Comfortable won't get too hot or too cold.
Slip resistance bottom to avoid slip.


text:
- Technical advance
- Open Access
- Open Peer Review
Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71
© Shi et al; licensee BioMed Central Ltd. 2011
- Received: 5 March 2011
- Accepted: 18 July 2011
- Published: 18 July 2011
Hand, foot, and mouth disease (HFMD) caused by enterovirus 71 (EV71) is very common in China. It is difficult to distinguish between EV71 and coxsackievirus A16 (CVA16) infections in clinical HFMD patients. Routine laboratory diagnosis of EV71 infection is time-consuming and requires expensive instruments. In this study, we have developed a one-step, single tube, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of EV71.
Six primers that can recognize 6 distinct regions on the VP2 gene of EV71 were designed for RT-LAMP assay. The amplification was completed by incubating all reagents in a single tube with reverse transcriptase and Bst DNA polymerase under the isothermal condition (60°C) for 60 min, and could be evaluated by using GoldView staining under a handheld ultraviolet torch lamp or electrophoresis analysis.
A total of 123 specimens collected from suspicious patients with HFMD were simultaneously detected by RT-LAMP and PCR fluorescence probing assay. The RT-LAMP amplified products containing EV71 were digested by HinfI and TaqI restriction endonucleases; in contrast, non-specific products with CVA16, coxsackievirus A4 and coxsackievirus B3 could not be detected in RT-LAMP assay. Meanwhile, RT-LAMP assay could amplify EV71 virus with a detection limit of 1 PFU/ml within 60 min. Compared with PCR fluorescence probing assay, RT-LAMP assay exhibited 98.4% identity during the detection of EV71 viral RNA without the missing of positive samples.
Our results indicated that RT-LAMP is a rapid, sensitive, specific and accurate method for the detection of EV71 in clinical specimens. Therefore, this developed method has potential application for rapid and comprehensive surveillance for EV71 infection, especially in developing country.
- Lamp Assay
- EV71 Infection
- Pharyngeal Swab
- TaqI Restriction Enzyme
- Magnesium Pyrophosphate
HFMD, a common illness in children, can be caused by many human enteroviruses such as coxsackie viruses A4, A5, A6, A10, A16, B1, B3, and EV71 [1–4]. Among these viruses, human EV71 and CVA16 are major causative agents of HFMD. EV71 and CVA16 infections in HFMD are indistinguishable. However, EV71 infection is frequently associated with serious neurological complications and fatalities [5]. EV71 was initially isolated from the stool of a 9-month-old infant with fatal encephalitis in California in 1969 [6]. Subsequently, the prevalence of EV71 infection has been reported in many countries and regions, such as Taiwan, Hongkong, Malaysia and Singapore, as well as Guangdong, Hunan, Jiangsu and Fuyang in China [7–13]. EV71 and CVA16 infections mainly occurred in children under 5 years old. However, patients infected with EV71 are liable to cause aseptic meningitis, encephalomyelitis, and pulmonary edema [14, 15].
Traditional EV71 infection is primarily dependent on serodiagnosis, and virus culture and identification. However, these methods are time-consuming and easy to produce cross-immune response with CVA16. Recently, reverse transcription-PCR (RT-PCR) and real-time PCR assays have been used for EV71 detection [16–18]. These nucleic acid amplification methods with intrinsic disadvantages of requiring sophisticated instrumentations and expensive reagents may not be the best choice for basic clinical settings in developing countries or in field situations. Therefore, it is necessary to develop a rapid, reliable, and simple molecular test to take the place of existing techniques. In 2000, a newly developed LAMP method with the characteristics of simplicity, rapidity, specificity, and cost-effectiveness has the potential to replace PCR [19]. LAMP is based on the principle of strand displacement reaction so that the stem-loop structure can amplify the target with high specificity, selectivity and rapidity under isothermal conditions. LAMP can also produce a large amount of target DNA and by-product magnesium pyrophosphate for the formation of turbidity. Therefore, LAMP assays have been widely used to detect a variety of infectious diseases, such as bacterial, fungus and viral infections [20–22].
Nowadays, the application of LAMP method in EV71 detection was less reported [23]. In the present study, we have developed a one-step, single-tube, and real-time RT-LAMP assay to detect EV71. The amplified products can be stained by double-stranded DNA binding fluorescent dye (GoldView stain), and observed through naked eyes under the UV lamp. Compared with PCR fluorescence probing assay, RT-LAMP had high sensitivity and specificity during EV71 detection, which is suitable for the application in primary health care agencies.
Specimen collection
A total of 123 suspicious patients with HFMD under 5 years old were enrolled in 2009 in Changzhou, China. This study was approved by the local ethics committee and all patients were provided a written informed consent. Totally 93 pharyngeal swabs, 20 vesicular fluid swabs and 10 fecal samples were collected from these patients within 4 days after the onset of infection, and stored in 3-5 ml of preservation solution (Hanks solution containing 10 μg/ml gentamicin and 0.25 μg/ml amphotericin B) at -70°C for analysis.
Virus isolation and identification
Specimens were inoculated into rhabdomyosarcoma (RD) cells for the isolation of EV71. Enterovirus strains were identified by using immunofluorescence test with EV71 monoclonal antibody (Chemicon International Inc.).
Primer design for RT-LAMP
The RT-LAMP primers
Primer names
Length of oligonucleotides (bp)
Primer sequences
Extraction of viral RNA
Prior to RNA extraction, fecal samples were treated with chloroform according to the protocol described elsewhere [24]. The genomic viral RNA was extracted from 100 μl of swab specimens or pretreated feces by using the QIAamp viral RNA extraction kit (Qiagen, Germany) according to the manufacturer's instructions. The RNA was eluted from the QIAspin columns in a final volume of 100 μl of elution buffer and kept at -70°C until further analysis.
Reaction system of RT-LAMP
The RT-LAMP reaction was performed in a 25 μl of reaction mixture containing 0.8 μM (each) FIP and BIP inner primers, 0.2 μM forward outer primer (F3) and backward outer primer (B3), 0.4 μM LF and LB (each), 0.4 mM deoxynucleoside triphosphates (dNTPs), 1 M betaine (Sigma, USA), 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH4)2SO4, 2.8 mM MgSO4, 0.1% Triton X-100, 0.5 μl of 8 U Bst DNA polymerase (New England Biolabs, USA), 40 U of reverse transcriptase (Invitrogen), and 5 μl of target RNA. Six temperatures (60, 61, 62, 63, 64 and 65°C) were screened during RT-LAMP assay, and finally the optimal temperature was monitored by agarose gel electrophoresis. Amplification was completed in PCR microtubes in an electric thermostatic water bath (Suopu, Shanghai, China) at the isothermal condition of 60°C for 60 min. GoldView dye (Sbsgene, China) was used to evaluate the reaction system with the reference of a negative control and a positive control. The samples with fluorescence change from orange to red or from orange to green were considered as the negative and positive reactions under ultraviolet (UV) light (254 nm) with a handheld lamp.
PCR fluorescence probing assay and nucleotide sequencing
The PCR fluorescence probing assay reagent (DaAn Gene, China) was commercially available. The cDNA was generated in a 20 μl of reaction volume for 25 min at 40°C using random primers and SuperScript II reverse transcriptase (Invitrogen) according to the instructions. The cycling conditions were composed of 5 min at 94°C, followed by 40 cycles with 93°C for 15 s, 55°C for 45 s, and 72°C for 1 min, and a final extension cycle of 72°C for 10 min. The PCR products were examined by Lighter cycler (Roche, Germany). Forward primer EV71-F: 5'-AAA GGT GGA GCT GTT CAC CTA CAT GCG CTT TGA C-3', reverse primer EV71-R: 5'-AAT CTG GCT TGG GGG CCC CAG GTG GTA CAA-3', and oligonucleotide probe EV71-P: 5'-CCC ACC GGG GAA GTT GTC CCA CAA TTG CTC C-3'. The specific oligonucleotide probes were added, and the results were considered as the positive for Ct value not higher than 35.1. All amplicons were sequenced with an ABI 3730 automated DNA sequencer (Applied Biosystems).
Specificity of RT-LAMP assay
A restriction analysis by using HinfI or TaqI endonuclease was used to validate the specificity of EV71 amplification reaction. Enzymatic reaction system is composed as follows: 2 μl of RT-LAMP product, 15 μl of ddH2O, 1 μl of 10 × buffer R, 1 μl of HinfI or TaqI for 10 U/μl (each). The digestion reactions of HinfI or TaqI were incubated at 37°C and 65°C for 2 h, respectively. Finally, 1.5 μl of digested RT-LAMP products were analyzed by agarose gel electrophoresis. Four virus strains of EV71, CVA16, coxsackievirus A4 (CVA4), and coxsackievirus B3 (CVB3) were provided by China Center for Type Culture Collection and Changzhou Center for Disease Control, respectively.
Sensitivity of RT-LAMP and PCR fluorescence probing assay
The EV71 or CVA16 was propagated in RD cells, and the titer was determined by plaque assay in vero cells [25, 26]. Totally 0.5 ml virus culture at various concentrations ranging from 105 to 0.1 PFU/ml in a serial 10-fold dilution pattern was detected by RT-LAMP and PCR fluorescence probing assay. The products of RT-LAMP were detected by agarose gel electrophoresis.
EV71 detection in clinical specimens by RT-LAMP and PCR fluorescence probing assay
Totally 93 pharyngeal swabs, 20 vesicular fluid swabs and 10 fecal samples with suspicious EV71 infection were extracted as described above and analyzed by RT-LAMP and PCR fluorescence probing assays with the reference of negative and positive control reactions containing CVA16 and EV71.
Statistical analysis
Statistically significant analysis of the clinical study was evaluated by using chi-square test. A significant difference was considered at the P value of less than 0.05.
The optimum reaction system of RT-LAMP assay
Restriction enzyme analysis of RT-LAMP products
Sensitivity of EV71 virus by RT-LAMP amplification
Detection of 123 clinical specimens
Comparison of 123 specimens from suspicious HFMD patients were analyzed by RT-LAMP and PCR fluorescence probing assay
Specimen type
Number of samples
PCR fluorescence probing
*Positive (%)
#Negative (%)
Positive (%)
Negative (%)
Pharyngeal swabs
41 (44.1)
52 (55.9)
39 (41.9)
54 (58.1)
Vesicular fluids
12 (60.0)
8 (40.0)
12 (60.0)
8 (40.0)
Fecal samples
5 (50.0)
5 (50.0)
5 (50.0)
5 (50.0)
58 (47.2)
65 (52.8)
56 (45.5)
67 (54.4)
Human enterovirus belongs to the RNA virus family Picornaviridae including polioviruses, type A coxsackieviruses, type B coxsackieviruses, echoviruses, and enterovirus types 68 to 71 (EV68-71) [15, 27]. Due to the prevalence of EV71 infection, time-consuming viral culture and serological tests for EV71, it is important to develop fast, efficient and sensitive diagnostic reagents [28, 29]. Although PCR technique has been widely used in the detection of infectious diseases and the real-time PCR assay has multiple advantages such as faster quantitative measurement, lower contamination rate, higher sensitivity, higher specificity and easier standardization, these nucleic acid amplification methods require high-precision instruments and skilled technicians [30, 31].
LAMP is a nucleic acid amplification method that relies on autocycling strand displacement DNA synthesis completed by Bst DNA polymerase. Because of high sensitivity and specificity coupled with the requirements for only a water bath and endpoint detection using a color-change reaction visible with the naked eye [32–34], LAMP assay has been applied for the analysis of various infections from Mycobacterium tuberculosis, Norovirus, Human papillomavirus, Cytomegalovirus, and Human immunodeficiency virus [20, 35–38].
In this study, a one-step and simple assay for EV71 virus without requirement of thermal cycling can be completed within 60 min in a single tube containing the mixture of buffer, primers, reverse transcriptase and Bst DNA polymerase. In order to ensure its accuracy and reliable amplification, it is very important to optimize the reaction temperature. At the optimal reaction temperature of 60°C, clear bands of amplified products were observed in gel electrophoresis.
Similarly, the optimal reaction time, magnesium and betaine concentrations were achieved. Except for amplified products detected by agarose gel electrophoresis as described above, two alternative detection methods could also be used for the evaluation. 1) Turbidity: the accumulation of magnesium pyrophosphate, a by-product of amplified DNA, results in the increase of the turbidity in samples. The turbidity in samples was evaluated by visual inspection. However, when the virus content of samples is low, it is difficult to observe the turbidity by visual inspection. 2) Fluorescence change: GoldView staining was used for evaluating the amplification. Samples with the fluorescence conversion from orange to green or from orange to red under an UV handheld lamp at a 254 nm were considered as the positive and negative, respectively. Different from traditional ethidium bromide (EB) for detecting double-stranded DNA, single-stranded DNA, and RNA, Goldview staining can emit green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. However, compared to EB with strong mutagen, Goldview has relatively fewer mutations and higher safety.
Particularly, similar clinical symptoms in infections caused by EV71 and CVA16 with high similarity in nucleotide sequences were observed. Therefore, frequent mis-diagnosis between EV71 and CVA16 infections was happened due to the difficulty in distinguishing both viruses [39]. However, exact nucleotide sequences of RT-LAMP products can be predicted from target DNA and primers. Thus, it is possible to predict the digestion outcome of the samples with restriction enzymes at the specific recognition sites. In the present study, the specific products could be digested by HinfI and TaqI restriction enzymes to result in a series of bands in agarose gel electrophoresis, in contrast, non-specific products could not be detected due to without digestion. Therefore, RT-LAMP assay can provide high specificity for the detection of EV71.
The sensitivity of RT-LAMP assay for EV71 detection was determined by testing serial 10-fold dilutions of virus with a detection limit of 1 PFU/ml. In order to evaluate the reliability of RT-LAMP, 123 suspicious samples were simultaneously detected by PCR fluorescence probing assay and RT-LAMP. Both test systems had 98.4% similarity without significant difference. With a detection limit of 1 PFU/ml, RT-LAMP assay exhibited much higher sensitivity than PCR fluorescence probing assay, as well as virus culture. Between two positive samples identified by RT-LAMP but PCR fluorescence probing assay negative, only one sample was verified as EV71 infection by virus culture and indirect immunofluorescence test, which further confirmed the higher sensitivity of RT-LAMP assay.
Besides pharyngeal, vesicular fluid and stool, RT-LAMP can be used to detect other samples such as cerebrospinal fluid, etc. However, the application of RT-LAMP in these samples needs to be further explored to provide more evidences in the early diagnosis of EV71 infection.
RT-LAMP assay is characteristics of high sensitivity, rapid detection, high specificity and low cost, which has considerable potentials for the detection of EV71 infection in the primary health care institutions, field environments or developing countries.
We thank Guanghua Luo and Yuehua Feng for their help in the LAMP assay and electrophoresis analysis.
Authors' Affiliations
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- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/11/197/prepub
Pre-publication history
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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@fourbitsdotcom is finally registered on Twitter. According to the dev's, the solution was to clear cookies, but that didn't work last night, so this morning I booted into my live CD, and did it in there. Note to the world: don't buy it when someone tells you that it's a cookie problem. And if it is, they're stupid for making it a cookies problem.
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Many people have heard about St. John's wort as an anti-depressant, but it also used for sore muscles, tendons and joints. I harvest my own from my gardens and also wild-gather in the neighborhood as it grows wild and is abundant in early July. To complement St. John's wort's anti-inflammatory and analgesic (pain relieving) properties, I've added Essential Oils for the same purpose.
Contains: olive oil infused with St.John's wort, Sweet Birch, Lavender, Tea Tree, Wintergreen Essential oils and Arnica Absolute.
Choose from the 2 oz. jar or 2.75 oz. stick.
The stick is unbreakable and glides right on the affected area. Offers hands-free application.
"I love "Oh my aching…" It is helping my bursitis. My rheumatologist liked it , too."
"We are amazed that "oh my aching…" works the best on the tendonitis and aching shoulder.
"I have osteoarthritis. Your remedy is incredible. 10-15 minutes after I put it on I had no pain in fingers and knees."


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Hands On Portland
Signups taken for MLK weekend of service
12/29/2015, 2:41 p.m.
The Martin Luther King Jr. Weekend of Service sponsored by the United Way's Hands On Greater Portland program is calling on volunteers to help with public service projects during upcoming holiday weekend of Jan. 15-18.
Residents can honor the legacy of Dr. Martin Luther King by signing up to participate in more than 65 volunteer service projects throughout the Portland and Vancouver metro region.
The efforts are hosted by a variety of partner organizations and open to all. The projects range from packaging food donations to cleaning up schools, from prepping books for kids in need to beautifying parks.
To sign up or learn more, visit the website unitedway-pdx.org/mlk-weekend.


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Harriette Algeo
Rahway High Class of 1964
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26 August until 1 October, 2006
Opening: Friday, August 25th, 6 pm
Harry Malter Family Park
Bosheidestraat 15 – 9070 Ghent
(Heusden) – Belgium
Open on Saturdays and Sundays,
from 11am to 6pm or by appointment.
Tel: 32 (0)477/764.623
info [at] beeldend.be
From the 26th of August until the 1st of October 2006, Zoo logical Garden brings 12 national and international artists together in the Harry Malter Family Park in Ghent (Belgium). The project consists of an exhibition, a catalogue, a weblog, video-screenings and discussions.
The participating artists and critics are:
Monika Bakke (Poland), Stefano Cagol (Italy), Vaast Colson (Belgium), Marta De Menezes (Portugal), Stefaan Dheedene (Belgium), Johan Grimonprez (Belgium), Katie Holten (Ireland), Shih Chieh Huang (Taiwan), Charlotte Léouzon (France), Alon Levin (Israel), Gert Robijns (Belgium), Anri Sala (Albania), David Shrigley (Great Britain), Superflex (Denmark)
Curator: Angelique Campens
The Harry Malter Family Park is a perfect illustration of the 'Disneyfication' of our society: reality has been replaced by a fake world consisting merely of cheerfulness, bliss and safety. Nature has been completely subjected to the fantasy view of modern man with all cruel and dangerous aspects being left out. The wild animals have been tamed and sit safely in their cages.
Except for showing various species of animals, the park is also fulfilling the role of a fairy land. The general atmosphere is rather absurd and unreal because of curiosities like the 'Mouseland', a King Kong slide, the painter Daniel D's fairground notices etc. These weird attractions might cause a feeling of alienation, something one does not experience when visiting a perfectly orchestrated Disneyland.
Entertaining tourists is not the only aim of an animal park however. The amusement is always combined with news and information about the park's research, enabling the visitor to form an idea of the breeding programmes, the behavioural patterns, the conservation and ecology. As such, the animal theme park is a combination of an amusement park and a zoo, with mainly commercial goals and an emphasis on entertainment.
The practice of collecting, describing and exhibiting are the core tasks shared by animal parks and museums. Just like in a museum, a zoo is a place where information and entertainment go hand in hand. An exhibition in a family park raises various questions however: how to organise an exhibition in a public space that looks surreal and where the prevailing atmosphere is so dominant? Is it possible to still add something to such a highly-charged setting, or does this lead to an even more exotic view? The Harry Malter Family Park is not a typical exhibition space, and is diametrically opposed to the concept of the 'white cube' in a gallery or exposition hall. Can there be a dialogue between a setting like this and the works of the artists? Or is it exactly in such places that a natural dynamic occurs, adding an extra dimension to the artistic project?
Zoological Garden shows a combination of work in situ and existing work that has been specially selected for this context. The project wants to bring artists and critics together to discuss one of the previously mentioned topics. On this basis, Monika Bakke (Assistant Professor, Department of Philosophy at the Adam Mickiewicz University in Poland) wrote the text 'The Predicament of Zoopleasures' for the catalogue. The artist Johan Grimonprez, together with Charlotte Léouzon (freelance curator and talent scout for Passion Pictures), compiled the DVD/Video Library 'Soo Logical' with movies like Gorillaz, El Manana, Pete Candeland; G Bush Jedi; Planet of the Apes; Halflife, Greenpeace, David Leaf; Remind Me van Röyksopp, … Superflex will bring their record compilation of animal noises a series that resulted from their bio-gas project in Africa.
Zoological Garden shows work by artists who create imaginary worlds and who put the relationship between reality and fiction into question.
More information:
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National Strategies and Guidance
Ending Violence against Women and Girls – Strategy 2016-2020
Published on the 8th March 2016 **NEW**
Responding to Domestic Abuse ~ a Guide for Health Professionals (Adobe 456 KB)
Produced by the Department of Health
Female Genital Mutilation Factsheet (Adobe 682 KB)
Produced by the Government Equalities Office
Teacher guide to domestic abuse in teen relationships
Home office website
Violence Against women And Girls Action Plan (Adobe 1.37 MB)
Produced by the Home Office
Violence Against Women and Girls Government paper (Adobe 1.37 MB)
Produced by the Home Office
Standing On My Own Two Feet (Adobe)
NSPCC report on Teen Intimate Partner Violence
Domestic and sexual violence, problematic substance use and mental ill-health are three issues that often co-exist. And when they do, things can become complicated.
Complicated matters: a toolkit and e-learning programme addressing domestic and sexual violence, problematic substance use and mental ill-health (designed by the Stella Project) are designed to 'uncomplicate' matters by raising professionals' awareness about how the three issues interlink and reflecting on the most effective ways to engage with individuals and families who are affected by these issues.
To this aim, both the toolkit and e-learning provide information on:
• the links between experiences of domestic and sexual violence, problematic substance use and mental ill-health
• ways to encourage survivors to engage with services and how to meet their needs
• ways to increase safety for survivors and their children
• holding perpetrators accountable for their own violent and abusive behaviour
• developing a holistic approach based on partnerships and integrated work
• practical, adaptable tools which enable organisations to improve policy and practice
This link will take you to the e-learning and toolkit: http://www.avaproject.org.uk/our-resources/good-practice-guidance–toolkits/complicated-matters-stella-project-toolkit-and-e-learning-(2013).aspx


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Tuesday, February 10, 2009
1966 American Standard Stratocaster
via this auction
"Vintage 1966 stratocaster. Original neck and Body. Original hardware. Original finish. The guitar plays like a dream. The issues are: The pickguard is probably a 62. Make/Model: Fender Stratocaster Year: 1966 Serial Number: 181892 Location of Manufacture: USA Neck/Fingerboard Wood: Maple neck and rosewood finger board Scale Length: 25 1/2" Nut Width: 1 11/16" Weight: 7.1 lbs Finish Color: 3-tone sunburst Pickup/Control Layout: 3 single coils/ 1 volume, 2 tone/ 5 way selector switch. Features: It does include the tremolo arm, but it does NOT include a case. But will be shipped in some kind of case to protect it. Condition: Original finish shows much playing wear. The pick-ups are original late 50's Fender. The pots date to 1980, 137-80XX. Non-original five way switch. All other parts are original 1966 Fender issue. The pickguard has some small cracks around the screw holes and the tip of the horn was missing. my Luthier repaired it with a similar peice. The original neck is perfectly straight and still in very good original condition."
Posted by vintageguitarz at 2/10/2009 09:07:00 PM
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Privacy Policy
JDL is a HIPAA-compliant business associate providing compliant cloud services and managed services. We respect your privacy and will never sell, rent, trade or otherwise share your contact information or other personal, private, confidential or proprietary information outside of our company. We sincerely appreciate your interest and your business.


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Jacksonville Sports Medicine Program Offers Free Screenings
Physicians, Nurses, and Other Volunteers Offer Expertise
Saturday, August 4, 2012, and Saturday, August 11, 2012, nearly 500 volunteers, including 85 volunteer physicians, provided free health screenings for approximately 1,500 local middle school and high school student-athletes in preparation for the upcoming school year.
Screenings for middle school girls and high school boys and girls took place on August 4. Screenings for middle school boys on August 11, both at Nemours Children's Clinic.
For 28 years, these screenings have been hosted by the Jacksonville Sports Medicine Program (JSMP) and its volunteers, which included pediatricians, family practice physicians, orthopaedic surgeons, sports medicine physicians, cardiologists and pulmonologists, pediatric nurses, clinical dietitians, athletic trainers, physical therapists, and rehabilitation specialists from multiple organizations in the community.
Organizations assisting with the effort are Wolfson Children's Hospital, Nemours Children's Clinic, Brooks Rehabilitation, Duval County Public Schools, and Duval County Medical Society. Event partners include Maggiano's Little Italy, BJs Wholesale Club, and Chick fil A.
Screenings, which are scheduled in advance by the Duval County Public Schools, along with local private schools, will be conducted at multiple stations. Student-athletes will have their height and weight measured, along with a blood pressure screening, nutritional counseling, a body fat analysis, a body systems screening, orthopedic assessment, and vision screening by the Lion's Club. The Community Asthma Partnership will screen kids at risk for asthma, and pediatric cardiologists from the University of Florida College of Medicine-Jacksonville and Wolfson Children's Hospital are available to screen for potential cardiac issues.
Jacksonville Orthopaedic Institute orthopaedic surgeon Stephen Lucie, MD, who serves as president of the Jacksonville Sports Medicine Program and has been participating in the local student-athlete screenings since they began in 1984
"Our goal at the Jacksonville Sports Medicine Program is to prevent injuries and a preparticipation screening is the first step in this process," Lucie said. "These comprehensive examinations are sports-oriented and are conducted by physicians and health care professionals who represent a number of specialties and who are interested in the health and well-being of our local student-athletes."
To date, the Jacksonville Sports Medicine Program, through the support of many participating physicians and healthcare professionals, have screened close to 50,000 student-athletes, with more than 40 schools participating.
About Nemours
Nemours is an internationally recognized children's health system that owns and operates the two free-standing children's hospitals: the Nemours/Alfred I. duPont Hospital for Children in Wilmington, Del., and Nemours Children's Hospital in Orlando, Fla., along with outpatient facilities in five states, delivering pediatric primary, specialty and urgent care. Nemours also powers the world's most-visited website for information on the health of children and teens, KidsHealth.org, and offers on-demand, online video patient visits through Nemours CareConnect. Nemours ReadingBrightstart.org is a program dedicated to preventing reading failure in young children, grounded in Nemours' understanding that child health and learning are inextricably linked, and that reading level is a strong predictor of adult health.
Established as The Nemours Foundation through the legacy and philanthropy of Alfred I. duPont, Nemours provides pediatric clinical care, research, education, advocacy and prevention programs to families in the communities it serves.