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A psychiatrist is a fellow who asks you a lot of expensive questions your wife asks for nothing
Author: Joey Adams
I myself spent nine years in an insane asylum and I never had the obsession of suicide, but I know that each conversation with a psychiatrist, every morning at the time of his visit, made me want to hang myself, realizing that I would not be able to cut his throat.
Author: Antonin Artaud (1896-1948)
Profession: French Theater Producer, Actor, Theorist
It is almost impossible to be a doctor and an honest man, but it is obscenely impossible to be a psychiatrist without at the same time bearing the stamp of the most incontestable madness: that of being unable to resist that old atavistic reflex of the mass of humanity, which makes any man of science who is absorbed by this mass a kind of natural and inborn enemy of all genius.
Author: Antonin Artaud (1896-1948)
Profession: French Theater Producer, Actor, Theorist
Psychoanalysts seem to be long on information and short on application.
Author: Gene Fowler (1890-1960)
Profession: American Journalist, Biographer
Every morning I woke in dread, waiting for the day nurse to go on her rounds and announce from the list of names in her hand whether or not I was for shock treatment, the new and fashionable means of quieting people and of making them realize that orders are to be obeyed and floors are to be polished without anyone protesting and faces are to be made to be fixed into smiles and weeping is a crime.
Author: Janet Frame (1924)
Profession: New Zealand Novelist, Poet
It might be said of psychoanalysis that if you give it your little finger it will soon have your whole hand.
Author: Sigmund Freud (1856-1939)
Profession: Austrian Physician - Founder of Psychoanalysis
Half a psychiatrist's patients see him because they are married -- the other half because they're not.
Author: Arnold H. Glasgow
Anyone who goes to a psychiatrist should have his head examined.
Author: Samuel Goldwyn (1882-1974)
Profession: American Film Producer, Founder, MGM
To study the abnormal is the best way of understanding the normal.
Author: William James (1842-1910)
Profession: American Psychologist, Professor, Author
Roses are red, violets are blue, I'm schizophrenic, and so am I.
Author: Oscar Levant (1906-1972)
Profession: American Pianist, Actor
If the nineteenth century was the age of the editorial chair, ours is the century of the psychiatrist's couch.
Author: Marshall Mcluhan (1911-1980)
Profession: Canadian Communications Theorist
If you are well off and can afford to spend ten or twenty-five dollars a day to hire some patient soul to listen to your troubles you can be readjusted to the crazy scheme of things and spare yourself the humiliation of becoming a Christian Scientist. You can have your ego trimmed or removed, as you wish, just like a wart or bunion.
Author: Henry Miller (1891-1980)
Profession: American Author
Psychiatry enables us to correct our faults by confessing our parents shortcomings.
Author: Laurence J. Peter
Work and love; these are the basics. Without them there is neurosis.
Author: Theodor Reik (1888-1969)
Profession: Austrian Psychoanalyst
Canst thou not minister to a mind diseased, pluck from the memory a rooted sorrow, raze out the written troubles of the brain, and with some sweet oblivious antidote cleanse the fraught bosom of that perilous stuff which weighs upon the heart?
Author: William Shakespeare (1564-1616)
Profession: British Poet, Playwright, Actor


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In march, I noticed that a google search for "staffan" yielded my blog at 23th place, down from 4th. I am now happy to announce that I'm yet again the fourth most important "staffan" on the web (at least on the US google.com). This might mean that Rasmus google-poisoning powers has worn off. I'm also number two in a search for "malmgren", which I think is an all-time high.
I think there's some kind of executive desk toy that displays things like stock indexes and website load with real-life analog dials, that get their information from some sort of radio network (I remember reading about it in Wired somewhere). I'd like to have one of those hooked up to my google rating.

subset: commoncrawl


text:
This is a follow up of my last post about my Grandma.
When my Grandpa's birthday came up this year, I decided to draw a picture of Grandma for him. As I was drawing and shading, I became quite nervous about the outcome – she just didn't look like the grandma I knew! I didn't know what to do but keep on. And I'm so glad I did. As soon as I put her mouth in (I always do the mouth last), I suddenly saw my dear Grandma looking back at me. It was a neat transformation of the picture I had just previously felt like throwing in the trash.
Here's how it went:
So here's a bit of advice to any of you who are artists, and those who want to be but feel like you're failing. Keep on until the picture is complete. If you put your mind to it, chances are slim that it will be a complete flop. Patience is something that any artist must have. Hours of work can turn a blank sheet of paper, into a beautiful picture.
"He that can have patience can have what he will."
Benjamin Franklin
Something I do to keep my mind stimulated (and keep me from getting bored after thirty minutes), is to listen to music. But don't listen to really fast or slow music. Fast music makes me feel like I can't work fast enough and becomes overwhelming. Slow music drags me down until I feel like jumping up and down and throwing the picture at the wall. Figure out what works best with you, and good luck!


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Sonar Film was created in 2003 by Britt Kramvig, Ingeborg Solvang and Rossella Ragazzi. They are all women and visual anthropologists and the company has produced already several documentaries of short length, made within an anthropological fieldwork and social scientific research, used in teaching aqnd seminars but also shown in festivals and movie theaters. "Firekeepers" is the first long-feature documentary of this company and was produced by the Norwegian Cultural Council, the Saami Parliament and the North Norwegian Film Centre.
Név:Sonar Film DA
Gyártó: Firekeepers Forgalmazó: Firekeepers


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- "I will smoke you like a Cuban cigar!"
- ―Sharpe[src]
Drill Instructor Sharpe is a Marine Drill Instructor stationed in Santego Military Base, Arizona. He introduces the PCV to Adrian Shephard and teaches him several training moves from surviving environmental hazards, night vision and firing a Desert Eagle to commanding a squad and using a radio.
List of appearances
- Half-Life: Opposing Force (First appearance)
- ↑ Stephen Bahl MobyGames profile
- ↑ Stephen Bahl as quoted on Marc Laidlaw Vault on the HalfLife2.net Forums
|Hazardous Environment Combat Unit
|Dwight T. Barnes · Sharpe · Adrian Shephard · Minor HECU members
|Combat Knife · Desert Eagle · Dam Turret · Double Turret · Dynamite · Glock 17 · Land Mine · Laser Tripmine · M198 Howitzer · M2 Browning Machine Gun · M202A1 FLASH · M249 Squad Automatic Weapon · M40A1 Sniper Rifle · Mk 2 Grenade · MP5 · RPG · Satchel Charge · Sentry Gun · SPAS-12
|AH-64 Apache · F-16 Fighting Falcon · M1A1 Abrams · M2A3 Bradley · M35 cargo truck · V-22 Osprey
|HECU Engineer · HECU Medic · HECU Sniper
|Powered Combat Vest · Radio · Tactical Map · Welding Torch
|Military Police · Santego Military Base
|Weapons (cut)
|Vehicles (cut)
|F-117 Nighthawk
|Classes (cut)
|Human Sergeant · Robot Grunt


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You are hereBlogs
4 season winter 3hr hit out
Didn't quite get my training right for this one but did OK.
I'd been doing 1 long ride (time wise) a week with a fair bit of climbing in it. but, being time strapped was relying on Tuesday night racing to keep my speed up and struggled to get time back that up. I really needed some more aerobic stuff and some intervals work thrown in.
In the weeks leading up I'd noticed my speed had dropped off on Tuesday nights and recognising my error managed to get a couple of spinnerval sessions on the trainer, a little late but better than nothing.
Tough one at Mt Annan
This was the last race in the 4hr Summer Series and was to be held at Mt Annan. I really like the twilight format and was looking forward to this one.
Coming into this race I was sitting in equal 3rd place overall in the series (mainly due to some riders only doing 1 or 2 races). I had hopes of an overall 3rd place but knew it would be a big ask.
6th is an improvement..
So Saturday evening was the final round of the Camelbak WSMTB 4 hour, despite the rains the organisers were on the ball and communicating early to notify that the event was going ahead as planned.
Thanks again to all the volunteers, WSMTB really seem to have the organisation well oiled and ran perfectly.
I'd arrived early to have a practice lap based on advise from some riding friends. I'm glad that I did, just to know how to ride the rocky sections and get a feel for the high number of switchbacks.
4th place in 1st solo 4hr
The goal going into this race was to simply finish on the bike, still peddling.
Once at the race I secretly had the goal to beat Vinno and his mate in the pairs.
smarter race. almost
Every week I head out to Rydal, set up the track and tell myself "I'm not going to sprint off the start this week. I haven't got that fitness."
Every week I line up at the start (no warm up), give the speech, say "Every one ready, set, Go!"
Every week we take off the red mist descends and I bust my gut to get a good spot into the single track, send my heart rate through the roof and then blow up trying to keep my spot.
every week I die in the arse in the second or third lap, get heart rate back under control then come home strongish but suffering
Kanangra 50km
This was the 2nd year of the Kanangra Classic and my 2nd attempt at the 50km.
My goal was to stick with Stephen for his 1st lap which I was hoping would be around the 2:20 mark.
About 1-2km in I found Steve's wheel in the 2nd group (maybe 3rd?) and stuck in his group for maybe another 6-7km but just didn't have the legs to stay with them. I dropped off the back and was in no-mans land by myself until almost the 1st turn off (11km mark). Just before the turn a chain caught me but they quickly fell apart on the 1st double track climb.
big foot family
Woke up early on saturday morning after a freezing cold night ,weather was fine and I was looking forward to a recce lap of the course and also to become more familiar with my new specialized 29 HT. after a big breakfast and a bit of socializing a group of us headed out on the course . with a few windy days prior to the event there were a lot sticks on the trail, unfortunatley a large stick found its way into my rear wheel and smashed my X9 rear mech bent the hanger and spokes . so I taped the bits to my seatpost shortened the chain and rode back to the campsite.
NSWMTB XCO Fitzroy falls
NSW MTB XCO Rd1 Superbowl
Not sure about the times however Ian King (kingy1) and Vincent Kier were both a fair way in from of me. Liked the track however didn't really find a happy rhythm until the second lap.
This berm was filling up with water during rain. The sandy soil drained quickly but it still needed a bit of help
First step was to make sure the inside right was the low point and dig cross drain
Next lay in some ag pipe
Then add a grate and cover with rocks


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The criteria for what establishes a genuine redundancy can be complex, however, learning how to carry out a lawful redundancy process will not only protect against potential legal claims – and there are several possibilities including unfair dismissal, adverse action, breach of an award or unlawful discrimination – but will also allow things to run more smoothly for all involved.The first step is to determine if the redundancy is genuine or not. Calling a dismissal a redundancy when it really was for a lack of performance will inevitably cause trouble.
Establishing a genuine redundancy
A redundancy can happen when an employer;
Redundancy can happen when a business;
A dismissal is not a genuine redundancy if the employer;
Consultation obligations
Procedural fairness yields great significance when determining if a redundancy is genuine or otherwise. The technical obligation to consult with affected employees is, strictly speaking, limited to situations where consultation about workplace change is required by an applicable modern award or registered agreement. If no award or registered agreement applies to the affected employees, then there is no strict requirement to consult, however organisation's may wish to conduct such a process regardless of the parameters imposed by relevant legislation as a matter of procedural fairness.
A consultation process should be conducted with a meaningful intention to reach the best decision for all rather than a simple "tick the box" to say that it has taken place.
Simply, the redundancy consultation process should involve providing the following information in writing to those affected by the changes;
If you require clarification or need assistance with implementing a genuine redundancy, please feel free to call us on (02) 8882 9694
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text:
Dear customer, If you come from United Kingdom and you want to buy wedding dresses bridesmaid dresses or prom dresses from our website, please click here: uk.millybridal.org
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I had to find something that was more country theme and relaxed fit. I BOUGHT this dress in my normal size. I was a tad scared it wouldn't fit but it fit perfectly! not to mention its so beautiful! I am so beyond thrilled! Fast shipping! I got it in 15 days.
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I rarely buy something online that is well priced and high quality. The dress fits beautifully and is the perfect length! The color is true as pictured.
The dress is amazing and charming, the figure made me slim, my friends was amazed my changes, thanks for offering me the best quality dresses.
Dress was just as described and delivery was very fast! The dress is absolutely GORGEOUS.
Just a quick note to let you know I received dress as ordered I have to say your service is spot on and I am delighted with the dress the quality is excellent.
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After wearing it,i have Received many compliments. Very flattering on any figure. I can't wait to wear it again. I think this dress is extremely flattering, love it so much


text:
WHEN Dawn, Lawrence Livermore's latest supercomputer, was installed in 2009, scientists at the Institute for Scientific Computing Research (ISCR) were among the first to put it to work. In doing so, they demonstrated an innovative parallelization strategy for simulating at unprecedented resolution and scale the hot, dense plasmas that will occur during fast ignition, the implosion–explosion process to ignite a fusion reaction, at the National Ignition Facility. Their strategy helped solve one of the most difficult scaling problems in numerical simulation: the efficient parallel calculation of long-range interactions. Long-range forces (such as electrostatic or gravitational) are relevant to a variety of modeling scenarios. Previous efforts to develop a fully scalable solution to this complex calculation have failed on less powerful machines.
ISCR's mission is to assemble and maintain multidisciplinary teams of researchers who work with Livermore project specialists in designing computer applications that address program needs. The institute also collaborates with students and other guests to advance computational capabilities. (See the box below.) Teams choose test-bed projects to push the envelope of application performance on today's supercomputers. Through this work, ISCR is creating a scientific computing capability that supports the Laboratory's missions and is developing the technology and expertise needed to effectively utilize next-generation computers, which are expected to expand from thousands to millions of processors.
Computers keep getting larger and more powerful, and Livermore excels at maximizing their potential. "Big computers do not arrive as turnkey devices," says ISCR Director Fred Streitz, who also leads the modeling and simulations group in Livermore's Physical and Life Sciences Directorate. "To use these machines to their utmost, exploring more complex problems with ever-increasing resolution, scientists must rethink old approaches and devise new algorithms."
Streitz is quick to credit his team of computational specialists for the institute's success. He lauds team leader Jim Glosli and team members Bor Chan, Milo Dorr, Erik Draeger, Jean-Luc Fattebert, Liam Krauss, David Richards, Tom Spelce, and Michael Surh for their ingenuity and dedication in completing the fast-ignition simulation. "Computers run 24/7," says Streitz, "and the researchers operating these machines sometimes must do nearly the same."
Glosli, Richards, and Streitz are veterans of a previous campaign, which ran for six continuous weeks and used the entire BlueGene/L supercomputer—more than 200,000 processors. In that groundbreaking simulation, they modeled up to 62.5 billion atoms of liquid aluminum flowing across liquid copper. For their effort, they were honored with the 2007 Gordon Bell Prize and with the Laboratory Director's Science and Technology Award in 2008.
Between them, ISCR team members have earned eight Gordon Bell prizes over the past five years. Named for C. Gordon Bell, one of the founders of supercomputing, this prize annually recognizes outstanding achievement in high-performance computing, with an emphasis on rewarding innovative science applications. Bell established the prize in 1987 to encourage the further development of parallel processing, the computer design philosophy that has driven high-performance computing since the 1980s.
A New Supercomputing Era
Sequoia will process calculations designed to build more accurate physical models of nuclear weapon detonations and will strengthen predictive capabilities by running very large suites of complex simulations. This work is a cornerstone of the National Nuclear Security Administration's Stockpile Stewardship Program to ensure the safety, security, and effectiveness of the U.S. nuclear weapons stockpile without underground testing.
Improving Traffic Flow on the Processors
Impossible to some, but not for the ISCR team. In analyzing the problem, the researchers noted that the Coulomb solver has two main computational pieces that, although largely independent, have different scaling behaviors. This observation led them to develop a heterogeneous decomposition process so that scientists can flexibly map, or "tune," the computational pieces to subsets of the hardware. The tunable approach provided excellent scaling of the Coulomb problem to thousands of processors. As a result, computational experts can for the first time incorporate long-range physics into extremely large-scale simulations.
Think of driving down a winding, two-lane road. No matter how fast you want to drive, the slowest car determines the speed for all cars in your lane. Computer scientists faced this problem in extending sequential applications to calculate complex interactions. Solving that problem led to parallel computing, which is like adding more lanes to the highway.
Even with today's massively parallel technology, the analogy still applies. "Using a single strategy to parallelize a simulation limits overall scalability because the least scalable component still holds back the entire code," says Glosli. "In our analogy, it's like having a truck in every lane." By assigning parts of a calculation to different processors—in essence, limiting trucks to just a few lanes—the team developed an approach for parallelization that allows multiple force terms to be computed concurrently. The overall calculation now scales effectively across hundreds of thousands of processors, maximizing the power of Dawn. The team was recognized as a 2009 Gordon Bell Prize finalist for developing this strategy.
"The heterogeneous decomposition of the computational problem and optimal mapping to hardware has far-reaching implications for scientific computing," notes Glosli. "It likely will affect the way future computer codes are developed for massively parallel environments." The flexibility of this approach allows more complicated models to be developed, and the technique can be applied on current and next-generation machines. The team has also developed methods to include shorter-range physical processes, such as radiation, recombination, ionization, and fusion, in the code. Says Streitz, "Our goal for this project is to deliver a comprehensive simulation tool for computing correlations and transport properties in burning plasma."
—Katie Walter
Key Words: Dawn, Gordon Bell Prize, Institute for Scientific Computing Research (ISCR), National Ignition Facility, plasma simulation, Sequoia, Summer Visitor Program.
For further information contact Fred Streitz (925) 423-3236 (email@example.com).
S&TR Home | LLNL
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Lawrence Livermore National Laboratory
Privacy & Legal Notice | UCRL-TR-52000-10-9 | September 9, 2010


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Traffic conditions in the area, especially along State Highway 66 between Lyons, Longmont, and I-25, are a major concern. The existing 1998 mining permit held by Martin Marietta Materials allows for an average of 200 daily round-trip truck trips between 8:00 AM and 6:00 PM, with a maximum of 240 truck daily trips to and from the site. That is one gondola truck entering or leaving the plant's entrance on SH 66 every three minutes. The permit also allows six additional train trips daily of up to 35 rail cars per trip through Hygiene and Longmont (three round trips per day). The additional trucks and trains would negatively impact traffic, safety, noise, and pollution in an already congested area whose population has increased over 40% since the permit's approval nearly two decades ago, according to the 2010 census. The proposed mining also would certainly impact other routes in the region that are increasingly strained by heavy traffic, such as Hwy 36 between Lyons and Boulder.
As of March 2017, CDOT was unaware of Martin Marietta Materials permit or plans to aggravate traffic conditions in the area. SOSvv attended the CDOT community presentation in March and informed CDOT of this imminent, potential threat. CDOT was already aware and very concerned about existing road hazards on SH 66, as detailed in their March 2017 presentation, even without the exorbitant increase in traffic and hazards in road conditions that this proposed mining operation poses.
Safety concerns
The SH 66 corridor poses a number of safety concerns:
Vehicular safety
Several intersection and mainline locations along SH 66 have a high number of crashes when compared to similar roadways. Between January and September of 2017 there were five traffic accident fatalities on Hwy 66.
Bicycle areas along the corridor have presented serious safety concerns, as revealed by recorded incidents and physical characteristics such as cross-street connections.
The Denver Post shares about the increases in fatalities associated with truck traffic in their May 5, 2019 article "Truck traffic kills more people, despite better inspections and more oversight. How safe are Colorado's roads?"
Bicycle safety
A majority of the SH 66 corridor is a heavily utilized by bicycles engaging in recreation, commuting, and cycling races and other events. There are many areas of the corridor that have insufficient shoulders to accommodate cyclists, especially non-advanced riders.
Pedestrian safety
There are a number of pedestrian destinations in the corridor, many of which do not have sidewalks between the destinations.
Mobility and access issues
The movement of people, goods, and services along the corridor has resulted in a number of mobility problems related to various transportation modes.
Traffic congestion, inadequate intersections that fail to accommodate users' needs, highway design, and unreliable travel times substantially impact the ability of people to move across and along the corridor.
The current number, locations, and design of public roadway accesses have contributed to operational and safety deficiencies relating to traffic along the corridor. There are individual private driveways, business accesses directly onto SH 66, and inconsistent access spacing, which leads to mobility and safety problems.
Transit service in the corridor is primarily focused on north-south connections and not local east-west service. There is currently a non-continuous connection of transit service providers in the corridor.
State Highway 66 (SH66) Future Planning Project
The notice below has just been sent out by the consulting firm that was hired by CDOT in September, 2015, to investigate the "issues" that exist along SH66. I have stated several times over, on this particular "chat" group, that something needed to happen – sooner than later. I have just spoken with Kelly Leadbetter at the consulting firm and have committed to her that "we" (that's the "collective community") would do our very best to send their firm CONSTRUCTIVE criticisms and suggestions. Please follow the link and read both the "Purpose and Need" and "Corridor Conditions Report" BEFORE sending in any comments so that you can more efficiently address the "issues". This is "OUR" opportunity to begin to interact with CDOT, through it's consultants, so as to allow the SH66 project to address all the important issues. We will be forming a small "investigative" group of folks who, hopefully, can consolidate ALL issues and present them collectively to the consulting firm, Boulder County and CDOT. To those that have recently expressed their concerns on "Nextdoor" – THANK YOU for being a part of "Our Community"!
The State Highway (SH) 66 Planning and Environmental Linkages (PEL) team would like your input! The project's Purpose and Need as well as the project's Corridor Conditions Report are now available for review on the project website,https://www.codot.gov/library/studies/co-66-pel Thank you, Kelly Leadbetter, AICP Felsburg Holt & Ullevig 6300 South Syracuse Way, Suite 600, Centennial, CO 80111 Phone: 303-721-1440 | Direct: 720-200-8978 Email: email@example.com


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A Betfair (SBR rating A-) trader has written to Sportsbook Review with a wagering complaint.
\r\n\r\n
The player registered at betting exchange Betfair on June 4 to wager on Louisiana track, race 1.
\r\n\r\n
Three minutes prior to post time, he states that Betfair removed a runner from the card (horse number 6) without any explanation. The horse ran and should not have been scratched, says the player.
\r\n\r\n
Because of the error, he closed out his position on the horse, then states that after the race ended, Betfair realized its error and reinstated the horse, then voided bets afterward which affected the player's wagers closing out his position on the horse.
\r\n\r\n
He lost €89.11 as a result of the confusion. He was offered a €25 compensatory bet by Betfair to be used in the sportsbook. The player states that he only uses the racebook and has no interest in the sportsbook, and that he should not be penalized because of the betting exchanges' error.
\r\n\r\n
Sportsbook Review is investigating this horse betting complaint.
\r\n\r\n
Betfair players in need of assistance should submit a sportsbook complaint.
\r\n


text:
YMCA Victoria
Health & wellness programs, memberships and locations
Find a pool, learn to swim, join a squad or swim laps
Early learning, outside school hours or occasional care
Helping young people reach their full potential
Eight great locations and camps tailored for you
Community and family support programs and events
Introducing Lincoln on the Bellarine
Building and facility maintenance that benefits youth
Locate a centre
Make a donation to support our cause.
Helping to provide access to opportunities for all
Donate, volunteer, sponsorship or attend an event
Your funds help to improve those in need
How to find help with accessing YMCA's services
Keep up to date with how your donations help
Benefits of getting started at a YMCA centre
Ready to get started? Find out how
Options to suit your lifestyle and activity level
Premier's Active April Special Offers
Pictured: The participants of the Men of Doveton program after one of their weekly sessions.
In the south eastern suburbs of Melbourne, men are helping change their community through the first ever Men of Doveton program.
Partnering with Doveton College, the 12-week free program has been designed to promote better mental and physical health amongst men in the community – championing positive change and creating social connection.
In these suburbs, many men are experiencing social isolation in their communities at increasing levels. High rates of alcohol abuse, smoking, obesity, as well as the pressures of housing affordability and cost of living are all compounding the problem – putting community mental health and wellbeing at risk.
The area also records the highest number of police reports for men's violence against women anywhere in the state.
Positive male role models are desperately needed, as well as education and understanding of mental health and healthy masculinity. This is where the YMCA has recently stepped in.
"Over each of the sessions in the Men of Doveton program we cover healthy eating, physical activity and mental health," explains Tori Norris, Community Engagement Coordinator in the YMCA Casey region.
"Through presentations, workshops, activities and other experiences, we are aiming to give men the support and guidance they need to live healthier, happier lives, which will hopefully start to influence other men in the community."
Of the 15 participants in this first ever program, 12 signed up solely to create new friends.
"Issues around social connection for men in the south east is just not being addressed. We know that healthy relationships are key in creating safer communities for families, and these men have shown real courage in signing up to this program," says Tori.
Melbourne Football Club have seen the power a program like this can have in the community, and have jumped on board as a partner. They have been facilitating sports nights, and giving away merchandise and signed footballs to participants.
Only a few weeks in, the program has seen some really positive outcomes.
"A ripple effect of change is starting to happen," says Tori.
"We are starting to build trust with these men, and they are seeing the benefits of the program already."
The program MC and YMCA staff member, Mo Mudaliar, has been providing extraordinary leadership to the group, creating a fellowship amongst the participants. It's through this work that these bonds are starting to happen – and will no doubt start to spread beyond the program.
A second Men of Doveton program has already been locked in for the second half of 2018, and as long as funding continues, there will be more in 2019 and 2020 as well.
"We're already looking at creating a Women of Doveton program down the line!" says Tori.
Stay tuned for some of the program's amazing stories later in the year!
Help your communityVOLUNTEER FOR THE YMCA
Find out more about the YMCA.Subscribe to our email newsletter
Discover a career full of diversity.WORK FOR THE YMCA


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Mold and mildew grow in moist areas, like a damp basement or the cabinet under your kitchen sink. Molds reproduce through tiny spores that float through the air. If you are sensitive to molds, breathing in these spores can cause:
- Nasal stuffiness
- Sneezing or wheezing
Dust mites the most common trigger of allergy and asthma symptoms also thrive in high humidity. They can live in bedding, curtains, and rugs, as well as the air in your home.
A dehumidifier brings down the level of moisture in your home, making it unfriendly to dust mites and limiting the growth of mold and mildew.
Use a dehumidifier if you have allergies or asthma and you live in a humid climate or have signs of moisture in your home, like:
* All information and source are for reference only.
|The Star Online >>


text:
4309 Players
Discover new land and experience exciting adventures and duels!
The West is waiting for you!
Play now!
Character name:
Login permanently
Change password
Found and expand a town with other players.
In this big world a lot of adventures and quests await you.
Are you are a prospector, double dealer or bounty hunter? It's up to you to decide which way you want to go!
Please select your game world:
© 2007 - 2017
InnoGames GmbH


text:
Thrive Church is this kind of church.
Thrive is a Church for people who don't go to Church or have given up on Church. For whatever reason, there are a ton of people who fall or fit into this place of life.
- Maybe they grew up in church and became disenfranchised with Church.
- Maybe once they hit 18 and went to college, a college professor convinced them that believing in God was illogical and they left the church and left their faith in God.
- Maybe they were hurt by the church and they vowed never to be a part again.
- Maybe the traditions seem out of place and they can't understand them.
- Maybe they think the church is filled with hypocrites and that churches are just against everything.
- Maybe Church has been boring to them in the past and they vowed not to step in.
Whatever place of life someone is coming from, Thrive exists to be a Church for people who have given up on Church and God.
So, if you have ever said…
- I will never step inside a church again
- The Church is boring and I would rather do something else on a Sunday
- Church is just for perfect people
- I have questions about God and I don't know where to get the answers
- I just want to laugh in church
- I don't own any nice clothes and that's why I don't go
- I want to go to a church where I understand what's being taught
- I want a Church that loves rock n roll
If you have ever said any of these things, Thrive Church is for you!
We're a Church where we proudly proclaim that…
'no perfect people are allowed'.
And, we're a church that's…
Simple, Fun and Real.
We're not a perfect church, but we love people selflessly we love God passionately and we would love for you to check us out!
See you on Sunday at either 900 or 1015 at Celebration Cinema in Mt Pleasant.


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IPSAS Reporting Tool
The SPARC General Purpose Financial Statement (GPFS) template is meant to aid states using the NCOA and wishing to produce its GPFS. The template allows the automatic and speedy creation of a State's four main statements of the GPFS on IPSAS Cash Basis including the Cash Flow Statement, a Statement of Assets and Liabilities, Statement of Consolidated Revenue Fund (CRF), and a Statement of Capital Development Fund (CDF) which are presented in a report referred to as 'The Final Accounts' or 'The Accountant General's Report'.
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Original Research ARTICLE
Magnesium lithospermate B protects cardiomyocytes from ischemic injury via inhibition of TAB1–p38 apoptosis signaling
- 1 Laboratory of Receptor-Based BioMedicine, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai, China
- 2 Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China
- 3 State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
Danshen has been used in traditional Chinese medicine for hundreds of years to treat cardiovascular diseases. However, its precise cardioprotective components and the underlying mechanism are still unclear. In the present study, we demonstrated that in a rat model of acute myocardial infarction, the treatment with magnesium lithospermate B (MLB), the representative component of phenolic acids in Danshen, significantly reduced the infarct size and the blood lactate dehydrogenase level. In contrast, tanshinone IIA, the representative component of lipophilic tanshinones in Danshen, had no such protective effects. Moreover, in the simulated ischemia cell model, MLB treatment considerably increased the cell viability and reduced the sub-G1 population and the apoptotic nuclei, indicating its anti-apoptotic effect. Further mechanism study revealed that the ischemia-induced p38 phosphorylation was abolished by MLB treatment. Interestingly, MLB specifically inhibited the TGFβ-activated protein kinase 1-binding protein 1 (TAB1) mediated p38 phosphorylation through disrupting the interaction between TAB1 and p38, but it did not affect the mitogen-activated protein kinase 3/6 mediated p38 phosphorylation. In conclusion, the present study identifies MLB as an active component of Danshen in protecting cardiomyocytes from ischemic injury through specific inhibition of TAB1–p38 apoptosis signaling. These results indicate TAB1–p38 interaction as a putative drug target in treating ischemic heart diseases.
Danshen, the rhizome of Salvia miltiorrhiza, is one of the famous ancient Chinese herbal medicines and is traditionally believed to "invigorate" the blood and reduce blood clotting. Remedies containing Danshen have been used customarily to treat coronary heart diseases, particularly angina pectoris and myocardial infarction (MI) (Ji et al., 2000). Although Danshen is officially listed in the Chinese Pharmacopoeia and is used widely and successfully in clinics in China, the exact mechanism for its therapeutic basis is poorly understood. Over the last 50 years, the chemical constituents and biological activities of Danshen have been widely studied. To date, more than 60 ingredients have been identified in Danshen, which are mainly divided into water-soluble phenolic acids and lipophilic tanshinones (Wang et al., 2007). Phenolic acids possess antioxidant and anticoagulant activities, whereas tanshinones show antibacterial, antioxidant, and antineoplastic activities. However, the main active component with cardioprotective effects on ischemic heart diseases and the underlying mechanism remain unresolved.
Ischemic heart diseases are characterized by an inadequate blood flow to the heart, which lead to limited availability of glucose and oxygen, delayed clearance of metabolic wastes and eventually massive death of cardiomyocytes. Earlier investigation indicated that cardiac ischemic injury is due to cell necrosis. More recently, apoptosis has been implicated in many forms of cardiac pathology and plays major roles in ischemia-induced cardiomyocytes death (Fliss and Gattinger, 1996; Liu et al., 2009). p38, one of the key factors in the mitogen-activated protein kinase (MAPK) signaling pathway, is activated during simulated ischemia and reperfusion (Ma et al., 1999). Pharmacological inhibition of p38 protects cardiomyocytes from apoptosis during simulated ischemia in vitro (Cheng, 2006). Interestingly, this p38 phosphorylation and activation are independent of the upstream mitogen-activated protein kinase kinase (MKK) 3/6, but dependent on the autophosphorylation of p38 through its interaction with TGFβ-activated protein kinase 1-binding protein 1 (TAB1) (Ge et al., 2002; Tanno et al., 2003). Thus, inhibition of the TAB1–p38 mediated apoptosis signaling has been implicated to be a potential way for the treatment of ischemic heart diseases.
In the present study, we investigated the protective effects of two representative components of Danshen, magnesium lithospermate B (MLB) and tanshinone IIA, in experimental models of ischemia. We demonstrate that MLB acts as one of the major active components of Danshen in protection of cardiomyocytes from ischemic injury and this is achieved by its specific inhibition of TAB1–p38 apoptosis signaling.
Materials and Methods
Sprague-Dawley (SD) rats were purchased from the Experiment Animal Center, Chinese Academy of Sciences in Shanghai. All animals were maintained under pathogen-free conditions prior to use. Male rats weighing 180–220 g were anesthetized with pentobarbital sodium (50 mg/kg) given intraperitoneally before surgery. All animal experiments were in accordance with the National Institutes of Heath Guide for the Care and Use of Laboratory Animals and were approved by Biological Research Ethics Committee, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
Rat Ischemia Model
Rat ischemia model was established by the left anterior descending artery (LAD) ligation as previously described (Connolly et al., 2006; Hu et al., 2009; Wang et al., 2009a). Briefly, after intraperitoneal drug administration, male SD rats were anesthetized and placed in dorsal decubitus supine position and a left thoracotomy was performed at the 5th intercostal space under aseptic surgical conditions. An intercostal retractor was positioned to maintain free access to the heart. The apex of the heart was partially positioned outside the thorax and the LAD was ligated approximately 2 mm from its origin. The heart was repositioned inside the thorax, which was subsequently closed. The air in the pneumothorax was then gently reduced by external compression. After breathing recovery, the animals were housed individually until the end of the experiment. Sham operated controls were treated in an identical fashion including administration of anesthesia but did not undergo ligature tie off. At the end of LAD ligation, the area at risk and infarct size of the left ventricular tissue sections was assessed by NBT staining as previously described (Zacharowski et al., 1999). The blood samples were collected 1 h after LAD ligation and serum lactate dehydrogenase level was measured spectrophotometrically using a lactate dehydrogenase assay kit (Shanghai Chemical Reagent Research Institute) according to the manufacturer's instructions.
Cell Cultures and Reagents
H9c2 cell (passage 18–25; American Type Culture Collection, Rockville, MD, USA), a clonal line derived from embryonic rat heart, was cultured in DMEM (Gibco-BRL) supplemented with 10% (v/v) heat inactivated fetal bovine serum and antibiotics, under an atmosphere of 95% air and 5% CO2 at 37°C. "Danshen Injection" was from Shanghai No.1 Biochemical Pharmacological Company. MLB with 100% purity and MLB with 83.2% purity used in cell model and animal models respectively were supplied by Shanghai Institute of Materia Medica, Chinese Academy of Sciences. Other reagents were from Sigma.
Simulated Ischemia in vitro
H9c2 cardiac myocytes were exposed to simulated ischemia as described (Fiedler et al., 2006; Wang et al., 2009b). In brief, the cells were switched from maintenance medium to an ischemic buffer and were incubated in a hypoxia chamber flushed with 5% CO2 and 95% N2 for indicated period of time. Control cells were cultured in a normal buffer and incubated at 37°C in an atmosphere containing 5% CO2 and 95% room air (normoxia).
Immunofluorescence Microscopy
Heart tissue sections were incubated with primary antibodies against total p38 or phosphorylated p38 and subsequently with an FITC-conjugated secondary antibody. Images were acquired with a confocal fluorescence microscope (Leica TCS SP2) as previously described (Kang et al., 2005).
The cells were harvested and washed with PBS (pH 7.2) twice and suspended in 80% ethanol at −20°C for 24 h. The cells were then thawed at room temperature, washed twice with PBS and re-suspended in the extraction buffer (0.2 M Na2HPO4, 0.1 M citric acid) for 5 min. The cells were stained with 50 μg/ml propidium iodide in PBS containing RNase A (100 μg/ml) for 30 min. The cell cycle distribution and quantitation of apoptotic cells were measured by a FACS calibur flow cytometer (Becton-Dickinson) (Du et al., 2004).
MTS Assay
The viability of cultured cells was evaluated by MTS assay (Promega) as previously described (Jing et al., 1999). Cells were seeded onto the 96-well plates at a density of 5000–10,000 cells/100 μl/well overnight. After treatment of various reagents, the cells were subjected to simulated ischemia for indicated time period. 20 μl of MTS solution was then added and cells were incubated at 37°C for 4 h. Absorbance at 490 nm was measured using a microplate reader.
Terminal Deoxynucleotidyl Transferase dUTP Nick End-Labeling (Tunel)
TUNEL was applied to detect DNA fragmentation that results from apoptotic signaling cascades. Cells were grown on glass cover slips for 24 h. Cells were then subjected to simulated ischemia for indicated time period and fixed with 4% polyformaldehyde. Cells were washed and permeabilized with 0.2% Tween in PBS containing 0.2% BSA. After fluorescein staining with TdT end-labeling cocktail (TdT buffer, Biotin-dUTP, and TdT) and avidin-FITC, DNA fragmentation was analyzed on a fluorescence microscope. Analysis of tissue samples was performed according to the manufacturer's instructions (Upstate).
Western Blot Analysis
Proteins were denatured, resolved on SDS-PAGE gels, and transferred onto nitrocellulose membranes. Primary antibodies were added at the recommended dilutions. Detection was performed with IRDyeTM800CW-conjugated secondary antibody and the infrared fluorescence image was obtained using the Odyssey infrared imaging system (Li-Cor Bioscience). In some experiments, protein bands were visualized by enhanced chemiluminescence.
Immunoprecipitation and in vitro p38 Activity Assay
After simulated ischemia, cells or homogenized heart tissues were lysed with ice cold immunoprecipitation buffer. Cell lysates were incubated with a total p38 antibody overnight at 4°C and then precipitated with protein A agarose beads for 2 h at 4°C. The beads were washed five times with lysis buffer and recombinant activating transcription factor-2 (ATF-2) fusion protein was added. p38 activity in immunoprecipitates was assessed by Western blot analysis of phospho-transcription factor-2 (Jing et al., 1999; Bonta et al., 2009).
Statistical Analysis
Results of the experiments were expressed as means ± S.E.M. Student t-test was performed for the statistical analysis of results. For all tests, a P-value less than 0.05 was considered statistically significant.
MLB Treatment Attenuates Ischemic Heart Injury in Rat
In order to test the protective effect of Danshen in myocardial ischemic injury, rat ischemia model was established by 1 h LAD ligation. "Danshen Injection," a total extraction from Danshen (Wu et al., 2007), MLB and tanshinone IIA, two representative components of Danshen, were applied just before the surgery at a dosage normalized to their natural composition in the plant (Figure 1). Compared to normal saline treated group, the heart infarct sizes in "Danshen Injection" and MLB (10 mg/kg) treated rats were reduced by 24.5% and 23.6% respectively, which is more effective than the control with a first-line drug nitroglycerin (30 mg/kg, infarct size reduction about 15%) (Figures 1A,B). In contrast, tanshinone IIA had no effect on reducing the infarct size. The reduction of the infarct area by MLB treatment was very significant and the differences can be clearly observed by visual examination (Figure 1C). The heart area at risk of acute MI was comparable between the normal saline group and drug treatment groups (data not shown). Blood samples were collected after 1 h LAD ligation and blood lactate dehydrogenase level, another marker for heart impairment during the MI (Garlid et al., 1997), was found to increase by 2.1-folds in the normal saline treatment group compared to the sham operation group. This increase was inhibited by MLB or "Danshen Injection" treatment but not by tanshinone IIA (Figure 1D). These data demonstrate that MLB acts as one of the major active components of Danshen in protection of cardiomyocytes from ischemic injury.
Figure 1. MLB treatment attenuates ischemic injury in rat myocardial infarction (MI) animal model. (A) Rats were intraperitoneally injected with normal saline (NS), tanshinone IIA (TIIA, 1 mg/kg), nitroglycerin (NG, 30 mg/kg), "Danshen Injection" (DSI, 120 μl/kg) and MLB (1–10 mg/kg), before LAD ligation. Heart infarct size was assessed after 1 h global ischemia by LAD ligation as a percentage of total left ventricular weight (%LV). (B) Infarct inhibition ratio by medicine treatment is depicted as a percentage of reduced infarct size compared to the infarct size in NS group. (C) Representative pictures of NS or MLB (10 mg/kg) treated rat MI hearts (red indicates the infracted area). (D) Lactate dehydrogenase (LDH) level in animal blood samples from (A). n = 8∼10; #, P < 0.001 compared with sham group. *P < 0.05; **P < 0.01 compared with NS group.
MLB Treatment Inhibits Apoptosis in Ischemic Cell Model and Rat Heart
It has been demonstrated that cell apoptosis is one of the major causes of cardiomyocyte injury during ischemic insults (Fliss and Gattinger, 1996). So we tested the ability of MLB in inhibiting ischemic induced apoptosis. H9c2 is a cardiac myoblasts cell line that is widely used to study the ischemic myocardial injuries in vitro (Bonavita et al., 2003; Agnetti et al., 2005). TUNEL assay revealed that simulated ischemia triggered apoptosis in about 35% of H9c2 cells. But in 10 μM and 30 μM MLB treatment groups, cell apoptosis was remarkably reduced to 22% and 12%, respectively (Figures 2A,B). This protection effect of MLB on H9c2 apoptosis was further validated by the FACS analysis. The percentage of sub-G1 cells were commonly used as a measure of apoptosis. Results indicated, at both 10 and 30 μM concentrations, MLB significantly reduced the sub-G1 population of H9c2 cells in simulated ischemia (Figure 2C). Moreover, TUNEL staining revealed cardiomyocytes apoptosis in the infarcted rat hearts was also inhibited by MLB treatment (Figure 2D), supporting the cardioprotection role of MLB in vivo.
Figure 2. MLB inhibits apoptosis in simulated ischemia treated H9c2 cells and ischemic rat hearts. (A) H9c2 cells were pretreated with NS or MLB (3–30 μM) for 30 min and subjected to simulated ischemia (16 h). Cell apoptosis was assessed by TUNEL assay. The mock treated cells were shown as control (CON). DAPI staining was used to reveal the total cell number. (B) Quantitative presentation of the TUNEL analysis shown in (A). Values are shown as a percentage of the TUNEL positive cell number to the total cell number. (C) Cell apoptosis assessed by FACS analysis. Cells were stained with propidium iodide and apoptosis was measured as the percentage of sub-G1 cells. (D) TUNEL assay was performed in rat hearts subjected to 24 h LAD ligation. Animals were pretreated with NS or MLB (10 mg/kg). Data shown were pooled from three independent experiments. #, P < 0.001 compared with sham group. *P < 0.05; **P < 0.01 compared with NS group.
MLB Inhibits Ischemia-Induced Apoptosis by Inhibiting p38 Phosphorylation and Activation
MAPKs play important roles in the ischemic injury of cardiomyocytes (Armstrong, 2004). In order to explore the potential signaling pathways involved in MLB-mediated cardioprotection, the H9c2 cells were pretreated with three types of MAPK inhibitors before simulated ischemia treatment. Interestingly, neither ERK inhibition by PD98059 nor JNK inhibition by SP600125 prevented cell death induced by ischemic injury (Figure 3A). However, p38 inhibition by SB203580 significantly increased cell viability to a similar extent as observed in MLB treatment. Furthermore, when cells were treated with MLB and SB203580 simultaneously, no additive or synergistic protection effects were observed. Cell apoptosis analyzed by TUNEL and FACS supports the MTS results (Figures 3B,C). These results suggest that MLB might exert its cardioprotective effect by inhibiting p38 signaling pathway. Western blot analysis was carried out to further validate this hypothesis. As shown in Figures 3D,E, pretreatment with MLB dose-dependently blocked ischemia-induced elevation of phosphorylated p38 level. In contrast, MLB had no effect on ischemia-induced ERK and JNK phosphorylation (Figures 3D,E). Similar results were obtained when infarcted rat hearts were subjected to Western blot analysis (Figures 4A,B). MLB significantly reduced ischemia-induced p38 phosphorylation but did not affect ERK and JNK phosphorylation in rat hearts. Immunofluorescence staining of phosphorylated p38 in infarcted rat hearts clearly demonstrated that MLB treatment dramatically reduced p38 phosphorylation (Figures 4C,D).
Figure 3. MLB inhibits simulated ischemia-induced apoptosis by inhibiting p38 phosphorylation in H9c2 cells. (A–C) H9c2 cells were pretreated with 10 μM SP600125 (SP, JNK inhibitor), 10 μM PD98059 (PD, ERK inhibitor), 1 μM SB203580 (SB, p38 inhibitor) and 30 μM MLB for 30 min and then subjected to simulated ischemia for 16 h. (A) Cell viability was determined by MTS assay. (B,C) Cell apoptosis was assessed by TUNEL assay and FACS analysis. (D) For Western blot analysis, H9c2 cells were pretreated with 30 μM MLB for 30 min and then subjected to simulated ischemia for 3 h. MAPKs phosphorylation was detected with specific antibodies against total and phosphorylated MAPKs (p38, ERK, JNK). Actin was also detected as a loading control. (E) Quantitative analysis of Western blot was performed with Scnimage software. Data shown are pooled from three independent experiments. #, P < 0.001 compared with the group without ischemia treatment. *P < 0.05; **P < 0.01 compared with ischemia treatment group.
Figure 4. MLB inhibits p38 phosphorylation and activation in ischemic rat heart. MAPKs phosphorylation in ischemic (1 h) rat heart tissues were analyzed by Western blot (A) or immunofluorescence assay (C). Antibodies against total and phosphorylated MAPKs (p38, ERK, JNK) were applied. Detection of actin served as a loading control. Quantitative analysis of Western blot was performed with ScnImage software (B). Quantification of immunofluorescence was performed with Image-pro software (D). Data shown are pooled from three independent experiments. (E,F) Whole rat hearts were homogenized 1 h after LAD ligation, and total p38 was immunoprecipitated with an anti-p38 antibody. Phosphorylated p38 and total p38 were analyzed by Western blot (E). P38 kinase activity was assessed in vitro by its ability to phosphorylate ATF-2 (F), #, P < 0.05 compared with sham group. *P < 0.05 compared with ischemia treatment group.
It has been established that p38 is activated by dual phosphorylation at 180Thr and 182Tyr residues (Jing et al., 1999), and the phosphorylation of p38 has been widely used to represent its activation. Direct measurement of p38 kinase activity was also carried out with immunoprecipitated p38 from ischemic rat hearts and recombinant ATF-2 was used as a substrate (Figures 4E,F). In agreement with our previous observation, ischemia clearly stimulated p38 phosphorylation as well as the phosphorylation of its substrate ATF2, whereas MLB inhibited this phosphorylation and therefore the activity of p38.
MLB Inhibits Ischemic Induced p38 Phosphorylation and Activation by Disrupting TAB1–p38 Interaction
In the MAPK activation cascade, p38 is generally phosphorylated and activated by MKK3/6 (Chang and Karin, 2001). In order to illustrate whether MKK3/6 is involved in the phosphorylation inhibition of p38 by MLB, sorbitol and TNF, which were reported to induce MKK3/6-dependent p38 phosphorylation (Wysk et al., 1999; Tanno et al., 2003), were applied. Strikingly, MLB treatment did inhibit the ischemia stimulated p38 phosphorylation but not sorbitol or TNF-stimulated p38 phosphorylation (Figure 5A). Similar results were obtained with SB203580, an inhibitor of p38 signaling which specifically inhibits ischemia-induced p38 phosphorylation, but not MKK3/6-mediated p38 phosphorylation (Gum et al., 1998). These findings suggest that MLB might function through a non-MKK3/6 pathway to interfere the p38 signaling.
Figure 5. MLB inhibits ischemia-induced TAB1–p38 interaction in H9c2 cells and rat hearts. H9c2 cells were pretreated with MLB (30 μM) or SB203580 (1 μM) for 30 min, then exposed to ischemia (3 h), sorbitol (0.1 M, 30 min), or TNF (10 ng/ml, 15 min) treatment. Total cell extracts were analyzed by Western blot for p38 phosphorylation (A). Simultaneously, cell lysate was immunoprecipitated with p38 (B) or TAB1 (C) -specific antibodies and the precipitates were analyzed for the presence of TAB1 or p38 by Western blot analysis. (D,E) Co-immunoprecipitation was performed with whole rat heart extract 1 h after LAD ligation and analyzed as described in (B) and (C).
Recently, a MKK3/6-independent model of p38 activation has been described where the scaffold protein TAB1 promotes p38 autophosphorylation through interaction with p38 (Ge et al., 2002). This TAB1 mediated p38 phosphorylation and activation has been demonstrated to play important roles in ischemia-induced heart injuries (Ge et al., 2002; Tanno et al., 2003; Li et al., 2005; Fiedler et al., 2006). Therefore, we tested the possibility whether MLB inhibits ischemia-induced p38 phosphorylation by interfering TAB1–p38 interaction. Consistent with the previous report (Tanno et al., 2003), we observed that ischemia (3 h) markedly stimulated p38 phosphorylation and its interaction with TAB1 in H9c2 cells (Figures 5B,C, lane 4). In contrast, TAB1–p38 interaction was not observed in H9c2 cells treated with sorbitol or TNF (data not shown), indicating that TAB1–p38 interaction is not regulated by MKK3/6 signaling. Interestingly, the TAB1–p38 interaction stimulated by ischemia was almost completely disrupted by MLB treatment (Figures 5B,C, lane 5). In contrast, SB203580, which inhibits p38 activity through binding to its kinase activity domain, had little influence on the interaction (Figures 5B,C, lane 6). We next examined whether MLB is also able to reduce TAB1–p38 interaction caused by MI in rat heart. As shown in Figures 5D,E, ischemia remarkably enhanced the interaction between p38 and TAB1, which was disrupted by MLB.
Taken together, our results demonstrate that MLB, one of the major active components of Danshen, protects myocardial injuries from ischemic insults in both animal and cell models. This protective effect of MLB is related to its ability to specifically inhibit ischemia-induced TAB1–p38 apoptosis signaling pathway.
Danshen has been widely used in China and, to a lesser extent, in Japan, the United States, and other European countries for the treatment of cardiovascular and cerebrovascular diseases. It has also been indicated for chronic hepatitis and liver fibrosis treatment (Liu et al., 2000), as well as inflammatory diseases such as lethal sepsis (Wan et al., 2006). The chemical constituents of Danshen are divided mainly into water-soluble phenolic acids and lipophilic tanshinones (Wang et al., 2007). The phenolic acids have diversed biological activities including antioxidation, anti-blood coagulation, vasodilation, cell protection, etc. Recent research revealed additional anti-tumor activity and anti-HIV activity of the phenolic acids (Jiang et al., 2005). The lipophilic tanshinones are also found to possess a variety of pharmacological activities such as antibacteria, antioxidation, anti-inflammatory and anti-tumor formation.
In the present study we demonstrate that MLB, the representative component of the water soluble phenolic acids in Danshen, has similar cardioprotective activity in the rat model of ischemia compared to the crude Danshen extract when used at a normalized dosage. In contrast, the tanshinone IIA has little effect when applied at a dosage comparable to its concentration within crude Danshen extract. Therefore, MLB, the representative of phenolic acids in Danshen, acts as the major effective component in the treatment of ischemic heart diseases. However, since Danshen has been used to treat many other cardiovascular diseases, tanshinone IIA as well as other components might function under different mechanisms that remain to be investigated.
Apoptosis has been demonstrated as one of the major causes of myocardial injury during ischemia (Thompson et al., 1995; Rodriguez et al., 2002; Garg et al., 2003). It is controlled by a network of signaling pathways, such as MAPKs, PI3K and Akt pathways. The classical MAPK signaling pathway is comprised of three major branches named ERK, JNK, and p38 (Chang and Karin, 2001). All three MAPK cascades have been directly implicated in regulating aspects of cardiomyocytes apoptosis and p38 pathway plays an active role in ischemia-induced cardiomyocytes apoptosis (Ma et al., 1999). Coincidently, our results herein showed that p38, but not the ERK and JNK inhibition, is involved in the MLB-mediated cardiac protection. Although PI3K and AKT signaling activation were reported to have cardioprotective effects (Kim et al., 2006). Our preliminary results show that MLB treatment has little influence on this signaling pathway (data not shown). If the specific inhibition of p38 by MLB is the underlying mechanism of Danshen's therapeutic efficacy, it is worthy to further explore the potential application of specific p38 inhibitors in the treatment of ischemic heart diseases.
P38 is generally activated by the transphosphorylation of its upstream MKK3/6 (Chang and Karin, 2001). However, recent observations indicate that MKK3/6 is not the only mediator of p38 phosphorylation in vivo. TAB1, a scaffold protein, also promotes p38 autophosphorylation through interaction with p38 (Ge et al., 2002; Tanno et al., 2003). TAB1-mediated p38 activation has been pointed out to play important roles in ischemia-induced myocardial injury (Ge et al., 2002; Tanno et al., 2003; Li et al., 2005; Fiedler et al., 2006). Consistent with these reports, we demonstrated that MLB treatment disrupts ischemia stimulated TAB1–p38 interaction in vivo. In vitro co-immunoprecipitation experiments revealed that MLB can not directly inhibit the TAB1–p38 interaction (data not shown), suggesting that TAB1–p38 upstream signal pathways are involved in the MLB activity. Previous reports showed that both PKGI (cGMP-dependent protein kinase type I) and AMPK (AMP-activated protein kinase) are involved in the regulation of TAB1–p38 interaction in the ischemic heart (Li et al., 2005; Fiedler et al., 2006). Whether MLB modulates TAB1–p38 interaction via PKGI and/or AMPK requires further investigation.
In summary, the present study identifies MLB as an active component of Danshen in protecting cardiomyocytes from ischemic injury in both animal and cell models. Further studies reveal this protection effect is mainly due to the inhibition of ischemia-induced p38 activation by MLB. More detailed analysis indicates that MLB does not affect MKK3/6-mediated p38 activation, but specifically inhibits TAB1-mediated p38 activation. Our results not only give light to a new mechanism that might lead to a better understanding of Danshen's therapeutic effect, but also point out TAB1–p38 interaction to be a putative drug target in treating ischemic heart diseases.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
We thank Shun-Mei Xin for her excellent technical support. This research was supported by grants from the National Natural Science Foundation of China (90713047), the Ministry of Science and Technology of China (2008DFB30150), Shanghai Municipal Commission for Science and Technology (08410703500, 08431910100, 09PJ1410000), Shanghai Postdoctoral Science Foundation (06R214161), Shanghai Key Laboratory of Signaling and Disease Research (09DZ2260100), and Roche RRDCC Basic Research.
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Liu, X. H., Xin, H., Hou, A. J., and Zhu, Y. Z. (2009). Protective effects of leonurine in neonatal rat hypoxic cardiomyocytes and rat infarcted heart. Clin. Exp. Pharmacol. Physiol. 36, 696–703.
Ma, X. L., Kumar, S., Gao, F., Louden, C. S., Lopez, B. L., Christopher, T. A., Wang, C., Lee, J. C., Feuerstein, G. Z., and Yue, T. L. (1999). Inhibition of p38 mitogen-activated protein kinase decreases cardiomyocyte apoptosis and improves cardiac function after myocardial ischemia and reperfusion. Circulation 99, 1685–1691.
Rodriguez, M., Lucchesi, B. R., and Schaper, J. (2002). Apoptosis in myocardial infarction. Ann. Med. 34, 470–479.
Tanno, M., Bassi, R., Gorog, D. A., Saurin, A. T., Jiang, J., Heads, R. J., Martin, J. L., Davis, R. J., Flavell, R. A., and Marber, M. S. (2003). Diverse mechanisms of myocardial p38 mitogen-activated protein kinase activation: evidence for MKK-independent activation by a TAB1-associated mechanism contributing to injury during myocardial ischemia. Circ. Res. 93, 254–261.
Thompson, C. B. (1995). Apoptosis in the pathogenesis and treatment of disease. Science 267, 1456–1462.
Wan, J. M., Sit, W. H., Lee, C. L., Fu, K. H., and Chan, D. K. (2006). Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury. Int. Immunopharmacol. 6, 750–758.
Wang, W., Peng, Y., Wang, Y., Zhao, X., and Yuan, Z. (2009a). The anti-apoptotic effect of heat shock protein 90 on hypoxia-mediated cardiom yocyte damage through the pi3k/akt pathway. Clin. Exp. Pharmacol. Physiol. 36, 899–903.
Wang, Y. P., Hang, P. Z., Sun, L. H., Zhang, Y., Zhao, J. L., Pan, Z. W., Ji, H. R., Wang, L. A., Bi, H., and Du, Z. M. (2009b). M3 muscarinic acetylcholine receptor is associated with beta-catenin in ventricular myocytes during myocardial infarction in the rat. Clin. Exp. Pharmacol. Physiol. 36, 995–1001.
Wang, X., Morris-Natschke, S. L., and Lee, K. H. (2007). New developments in the chemistry and biology of the bioactive constituents of Tanshen. Med. Res. Rev. 27, 133–148.
Wu, B., Liu, M., Liu, H., Li, W., Tan, S., Zhang, S., and Fang, Y. (2007). Meta-analysis of traditional Chinese patent medicine for ischemic stroke. Stroke 38, 1973–1979.
Wysk, M., Yang, D. D., Lu, H. T., Flavell, R. A., and Davis, R. J. (1999). Requirement of mitogen-activated protein kinase kinase 3 (MKK3) for tumor necrosis factor-induced cytokine expression. Proc. Natl. Acad. Sci. U.S.A. 96, 3763–3768.
Zacharowski, K., Olbrich, A., Otto, M., Hafner, G., and Thiemermann, C. (1999). Effects of the prostanoid EP3-receptor agonists M&B 28767 and GR 63799X on infarct size caused by regional myocardial ischaemia in the anaesthetized rat. Br. J. Pharmacol. 126, 849–858.
Keywords: magnesium lithospermate B, ischemia, apoptosis, p38, TAB1
Citation: Du C-S, Yang R-F, Song S-W, Wang Y-P, Kang J-H, Zhang R, Su D-F and Xie X (2010) Magnesium lithospermate B protects cardiomyocytes from ischemic injury via inhibition of TAB1–p38 apoptosis signaling. Front. Pharmacol. 1:111. doi: 10.3389/fphar.2010.00111
Received: 20 June 2010;
Paper pending published: 09 July 2010;
Accepted: 03 August 2010; Published online: 24 August 2010
Edited by:Tong-Hui Ma, Jilin University, China
Reviewed by:Hong Yang, Liaoning Normal University, China
Yuanjian Li, Huanan University of Science and Technology, China
Copyright: © 2010 Du, Yang, Song, Wang, Kang, Zhang, Su and Xie. This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
*Correspondence: Xin Xie, Shanghai Institute of Materia Medica, 189 Guo Shou Jing Road, Shanghai 201203, China. e-mail: email@example.com and Ding-Feng Su, Department of Pharmacology, School of Pharmacy, Second Military Medical University, 325 Guo-He Road, Shanghai 200433, China. e-mail: email@example.com


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Thursday, April 2, 2009
Santa Fe
This time last week, THOMY and I were hightailing it out of Santa Fe a day early to avoid the snow storms that were predicted to blind us along our entire route home very soon. At 2:30 Friday morning, we rolled into our driveway without one drop or flake having fallen on us. When we woke up about eight hours later, and for most of the weekend, all was ice and gray skies.
In Santa Fe, we stayed in an apartment, in a four-plex building which was once housing for the military in Santa Fe...a verrry old place. Having such a casita within the edges of Old Santa Fe was supposed to be a big advantage since the narrow, confusing streets in this city center discourages driving. THOMY and I were just going to walk everywhere. I love doing that because I'm not so thrilled with driving in any unfamiliar place; it punches all the zen out of a vacation, you know?
So it was supposed to be a bonus. However, I had developed some trouble with my back before we left for Santa Fe, and it only got worse once we were there. I even brought my electric heating pad, which I began to endearingly call my tuffet. By Tuesday morning, though, I was in tears with pain.
I persevered, of course. I was on vacation, dadgumit--the farthest West and the highest up in elevation I'd ever been for a lowlands girl. So I pulled on my big girl panties--no trifling feat--sucked in the air-deficient air, and I loped from block to block. I sat every few hundred feet to calm my lungs, vertebrae, and occasionally my husband before hoisting myself up and doing it some more. Consequently, many of my touristy photos are close ups of things I found around me as I rested.
In my hometown, geese are more of an annoyance than pigeons. So the pigeons of Santa Fe's central Plaza amused me for awhile.
The left one seems to be looking at his neighbor, thinking, "Sheesh, man. Table manners!"
We went to see The Miraculous Staircase in Loretto Chapel. (Visit the website to read the story behind it.) The chapel is no longer a workaday holy place, but quietness remains in the atmosphere there.
Underside of the staircase.
On Museum Hill, I rested in the courtyard between four amazing museums. And this is what I saw near me.
Once again, I have no idea what the name of the plant is. But I like it.
And just to make this entry narrowly related to creativity, I found the most awesome yarn shop in Santa Fe: Miriam's Well, which is also the Santa Fe School of Weaving. Here is her courtyard.
I bought a purse there made by a Guatemalan woman who is named on the label inside. The label isn't in English, but I think the gist is she is part of a creative cooperative through the Methodist denomination. I like the personal weight of carrying around her name along with all the things I need in that bag.
The bag, the yarn, and a small glass vase I brought back from Santa Fe.
If you'd like to see more of my touristy photographs, you're welcome to view my Santa Fe Flickr album.
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Entry requirements
Candidates should have a degree or equivalent in an appropriate subject from an approved educational establishment/professional qualifications or experience sufficient to satisfy the Head of Division and Course Director of the applicant's fitness to pursue the course of study.
Months of entry
January, September
Course content
This is a flexible MSc course designed to provide healthcare professionals with a multi-disciplinary perspective on genomics and its application in medicine. You will be trained in state-of-the-art genomic platforms and informatics tools and how to apply them in the analysis and interpretation of whole genome sequence data from patients. The course is taught by a team of experts of international calibre in a stimulating research environment next to the Headquarters of Genomics England.
Qualification and course duration
Course contact details
- Name
- Dr Nina Ravic
- firstname.lastname@example.com


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Toys involve some very complicated engineering. In this week-long workshop we'll explore different types of toys and expose your curious scientist to the manufacturing process they entail. We'll explore the engineering design process as we refine and modify toy ideas. From designing your own toys to building ours you are sure to have tons of fun building and making with us. Activities may include: -Learning about materials and their properties -Designing simple push/pull toys and then adding linkages -An Introduction to simple circuits -Discovering Hydraulics -Learning about Gears and what they do -Experimenting with robots
Website: www.scienceseeds.com/program-registration/?ee=593
Location: scienceSeeds Main
Address: 29 Emmons Drive
Suite G10
Princeton, New Jersey 08542
Age: 6-8
Registration: Registration Required
View business listing for scienceSeeds


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Nadia van der Merwe
Leader: Fish Hoek, Simon's Town
I am a Food and Beverage Instructor, who basically live in restaurants and is often surrounded with really good food. For this reason I had to find effective way in managing my weight and started off by running short distances.
My motto has always been to ensure that my running sessions was fun and exciting in order for me to build a good relationship with running and today I can comfortably run a half marathon, with more aspirations. Even though I have had my fair share of running injuries, running has not only taught me how to look after my body, but it has also helped me to perform better physically and mentally. I have learned allot about self-discipline and having a strong and positive mind-set. My running schedule comprises of an average 7 hour training week that includes speed and endurance training with professional coaching on Thursday's.
I would like to invite you to challenge yourself and join me for a run every now and again, to teach you that running can be fun and that you should never under estimate the power of your body. You never know what great achievements you can accomplish, if you only dare to believe in yourself.
Click on the Facebook Button below to join Nadia's CMIYC Group.


text:
The material below corresponds with the ACE curriculum frameworks for Digital Communications III and Technology Design and Applications.
Web Design PowerPoint, Worksheet
Website Design Rubric
Web Design Vocabulary
Topics for Websites
I've taught this unit using a variety of software, from iPad apps, iWeb to online software. Weebly and Wix get my vote.


text:
The Creative Commons copyright licenses and tools forge a balance inside the traditional "all rights reserved" setting that copyright law creates. Our tools give everyone from individual creators to large companies and institutions a simple, standardized way to grant copyright permissions to their creative work. The combination of our tools and our users is a vast and growing digital commons, a pool of content that can be copied, distributed, edited, remixed, and built upon, all within the boundaries of copyright law.
License design and rationale
All Creative Commons licenses have many important features in common. Every license helps creators — we call them licensors if they use our tools — retain copyright while allowing others to copy, distribute, and make some uses of their work — at least non-commercially. Every Creative Commons license also ensures licensors get the credit for their work they deserve. Every Creative Commons license works around the world and lasts as long as applicable copyright lasts (because they are built on copyright). These common features serve as the baseline, on top of which licensors can choose to grant additional permissions when deciding how they want their work to be used.
A Creative Commons licensor answers a few simple questions on the path to choosing a license — first, do I want to allow commercial use or not, and then second, do I want to allow derivative works or not? If a licensor decides to allow derivative works, she may also choose to require that anyone who uses the work — we call them licensees — to make that new work available under the same license terms. We call this idea "ShareAlike" and it is one of the mechanisms that (if chosen) helps the digital commons grow over time. ShareAlike is inspired by the GNU General Public License, used by many free and open source software projects.
Our licenses do not affect freedoms that the law grants to users of creative works otherwise protected by copyright, such as exceptions and limitations to copyright law like fair dealing. Creative Commons licenses require licensees to get permission to do any of the things with a work that the law reserves exclusively to a licensor and that the license does not expressly allow. Licensees must credit the licensor, keep copyright notices intact on all copies of the work, and link to the license from copies of the work. Licensees cannot use technological measures to restrict access to the work by others.
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This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.
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This license lets others remix, tweak, and build upon your work even for commercial purposes, as long as they credit you and license their new creations under the identical terms. This license is often compared to "copyleft" free and open source software licenses. All new works based on yours will carry the same license, so any derivatives will also allow commercial use. This is the license used by Wikipedia, and is recommended for materials that would benefit from incorporating content from Wikipedia and similarly licensed projects.
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This license is the most restrictive of our six main licenses, only allowing others to download your works and share them with others as long as they credit you, but they can't change them in any way or use them commercially.
For more details Vനsit


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This quick bread is low in calories but still high in taste! You'll love this moist, dense bread.
1 cup sugar
1/4 cup light butter softened
3 ripe bananas mashed up (I always use 4-5)
1/4 cup low fat milk
1/4 cup low fat sour cream
2 large egg whites
2 cups all purpose flour
1 teaspoon baking soda
1/2 teaspoon salt
Cooking spray
Preheat oven to 350.
Beat sugar and butter at medium speed until well-blended. Add banana, milk, sour cream, and egg whites; beat well.
Lightly spoon flour into dry measuring cups; level with a knife. Combine flour, baking soda and salt. Add flour mixture to sugar mixture, beating until blended.
Spoon batter into a 9 x 5 inch loaf pan coated with cooking spray. Bake at 350 for 1 hour and 10 minutes or until a wooden pick inserted in the center comes out clean. Cool 10 minutes in pan on wire rack; remove from pan.
Quick banana tip: if you have bananas that are getting too ripe to eat, throw them in the freezer. Yup just in the skin, don't do anything to them. When you are ready to bake with them, just defrost and peel. They will be a little more liquid-y than normal but otherwise they work fine. Don't be alarmed when the banana turns completely black in the freezer. Recipe from Cooking Light, 2000.


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Blue Duck - Whio
West Coast NZ
New Zealand's endemic Blue Duck lives on fast-flowing mountain rivers & streams where they feed on insect larvae on the streams rocks.
Mandarin Duck
Lake Rotoiti - Nelson Lakes
Australasian Shoveler
New Zealand Scaup - Adult Male
Travis Wetlands Christchurch
New Zealand Scaup - male
Australasian Shoveler - Adult female
Australasian Shoveler - Adult female preening
Botanical Gardens Christchurch
Grey Teal
Male - Travis Wetlands, Christchurch
NZ Scaup - female
Northbrook Wetlands Canterbury
NZ Scaup - male
Northbrook Wetlands
New Zealand Scaup
Travis Wetland - Christchurch
New Zealand Scaup - Male
Northbrook Wetlands, Canterbury
Male - Travis Wetlands
Brown Teal
A pair of Brown Teal in Northland, the male is in non breeding plumage.
On a stream at Teal Bay, Northland
Australasian Shoveler, male - non breeding
Chilling out at the Botanical Gardens - Christchurch
Travis Wetlands - Christchurch
Lake Forsyth Little River
Paradise Shelduck
Female with duckings - Northbrook Wetlands Canterbury
Paradise Shelduck - female
Lake Forsyth, Canterbury
Paradise Shelduck - Male
Mallard Ducking
Northbrook Wetlands, Canterbury
Mallard family
Mallard duck
Mallard Duck
Female preening at Lake Forsyth, Canterbury
© Kathy Reid Photography. All rights reserved
duckshoveleraustralasian shovelerfemale shovelerwater birdbird photography


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Speeches & Discussions
Materials for a culture of decarbonised products
17th of January 2018, 1 p.m. – 1:45 p.m.
imm cologne, "The Stage", hall 3.1, KölnMesse
Green lacewing silk, in vitro leather, chicory plastics, 4D textiles: the material developments of recent months are really something. This is because they point with unprecedented clarity to a transformation of our materials and product culture. With the signing of the Paris Climate Agreement, the community of nations has agreed to the decarbonisation of our industrial system. What this might mean can be seen in the outstanding developments of several scientists and designers. New mechanisms of organic fabrication point out a path in the direction of closed material cycles. Through the use of natural waste materials, a new category of organically-based plastics is being prepared. The usage of biogenic material solutions allows us to direct our attention to the copying of functions from nature.
Dr. Sascha Peters is going to give an outlook on current material trends for design and furniture in the lecture forum "The Stage" at imm Cologne.
event program of imm Cologne 2018: www.imm-cologne.de
image: Organic fibers from green lacewing silk (source: Fraunhofer IAP)


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Home > Cage & Aviary Supplies > Cage & Aviary Bird Feed > Versele Laga
Available - usually in stock
550g Versele Laga Clay Bloc Amazon River pickstone for parrots and large parakeets.
Clay Bloc Amazon River is a pickstone based on clay, ideal for parrots and large parakeets. Source of minerals and trace elements. Ingredients: loam, oystershell, seashells, redstone, stomach gravel.
Excellent productWould prefer the bigger blocks if they do them
My birds cant get enough of this & eat it so it turns to powder.My breeders LOVE it as much.I would always recommend this product to anyone
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Member Blogs > Journey of a BooksellerAn Amish Family Christmas by Shelley Shepard Gray
Wed, 26 Oct 2016 09:00:00 PermalinkJulia was young when she got involved with a man who projected one side of his personality to the public and another to her. She even went so far as to get engaged. And then she let him take her virginity. But when she got pregnant, she knew she couldn't marry him. He was abusive and controlling. He hit her when she told him she was pregnant. She knew if she told her parents they would make her marry him. So she ran...
Avon and Edelweiss allowed me to read this book for review (thank you). It has been published and you can get a copy now.
She first lived with an elderly couple that ran an old motel. She cleaned the rooms, they let her stay in one. And they loved her new baby, Penny. She stayed as long as she could but they retired and sold the motel so she moved on.
She found a new job, a small house she could afford and she spent a lot of time with Penny. She didn't socialize or contact her family. She was still trying to keep her child and herself safe. Then she locks the door to her house and forgets to take the key. She's scrambling around trying to find a rock to break a window when her neighbor, Levi comes home and offers to help.
She doesn't want to invite him in but her daughter is taken with his attention and it's easier than standing in his way. Both Levi and Julia have hurts from the past they need to move past, so it's not an easy romance. But they do both find themselves attracted to their personalities and kindness.
The romance is slowly moving along and then Penny gets ill. He takes them to the hospital for treatment and the nurse that fills out the paperwork points out the child has a different name from the mother. That's when Levi finds out she's not a widow; it's an illegitimate child. He's staggered. She's ashamed. And then when things couldn't get worse, Luther shows up. He intends to take her home with him and torture her some more.
I didn't like having Luther in the story. I know these things happen everywhere but I hate stalkers or abusers. However, the romance between Julie and Levi was sweet and nice and this Christmas time story ends well. That's what counts to me.


text:
Business Enabler / Professional Connector (Revenue Sharing Model)
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As Business Enabler, you are responsible to develop new partnerships with selected solution providers as well as introducing our Entrepreneur and Executive members to the solutions that most serve their needs.
We operate in a revenue sharing model where you receive a percentage of all the revenues you generate.
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jamesaultman Profile Limit Reached Log In needed
James A
For Accurate Solution Be in contact with the technical support group of McAfee Activation Help to manage email issues. To ask your record inquiries, refresh and introduce related question make a call at sans toll number +1-877-301-0214 and acquire the required technical support. From any piece of USA, you can without much of a stretch make a call to our antivirus bolster group.


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Since 1924, ABUS has provided the good feeling of security worldwide. Engineered in Germany, ABUS security products are the highest quality, incredibly reliable, easy to operate, and offer a long service. In order to meet the growing requirements of private and commercial users, ABUS provides a large product range of innovative solutions for home security, safety lockout, commercial security and recreational security. The ABUS group is made up of more than 20 partners worldwide and is headquartered in Wetter, Germany.
Since 2007 Aim Sports Inc. has been a leader in bringing innovative, quality, affordable products to all varieties of American Shooters. Never afraid to introduce unique items to even the narrowest segments of the shooting sports world, we continue to keep our ears to the ground for feedback in our ever improving and ever expanding product line. Our design team has decades of experience, from law enforcement to competition shooting, and everything in between. With the drive to stay on the cutting edge, we are sensitive to the trends and new directions of this rapidly evolving industry. Whether it is unique modifications for classic surplus rifles, or KeyMod and M-Lok compatible rail systems, our customers know why Aim Sports products are sold on five continents, and why consumer loyalty drives us to introduce increasingly advanced firearm accessories each year.
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Personal Training
We understand that group fitness classes may not be for everyone. That's why we offer one-on-one training (or small group training for you and a few friends/family members) which includes a personalized program to fit your abilities and goals.
Click on a name below to check out the trainers and schedule a session today!


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Well I know a lot of people probably celebrated this weekend. But I am still counting down to the day itself.
I saw Poltergeist when I was really little and spent the next couple of years shutting off TVs the second they went to static. There are so many great scenes from this movie, but this one is the first real scare. The hand coming out of the TV does it every time.


text:
BEO Ripper
BEO Ripper
Daz/Poser | 3D models | 21.77 MB
It is the way of all things great and small, to adapt, to evolve, to change. The BEO Ripper is the next evolution of the BEO2K10 series. This is a detailed stand alone figure that would strike fear into the hearts of men.
Detail link:


text:
Fawn Interiors Studio used Little Greene in their recent design project to create a classic kitchen scheme for a thatched cottage.
They used the classic off-black colour, 'Obsidian Green' which provided a dramatic backdrop to natural wood furnishings and khaki in the 1970s. This was accompanied by the deep grey, Scree and the perfect neutral, Loft White.


text:
|General Wrestling Any non-WWE/TNA wrestling discussion goes in here. This includes wrestling history.
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|23 Mar 2011, 05:20 PM
|#1 (permalink)
Fresh Noob
Join Date: Mar 2011
Posts: 2Rep Power: 0
Which wrestler in ROH has the most potential? +BQ?
BQ1) Do you think Eddie Edwards should have won the title?
BQ:2) Which stars would you like too see switch from ROH to WWE?
BQ:3) Which match at Wrestlemania are you most looking forward too and why? Give your prediction
|23 Mar 2011, 05:33 PM
|#3 (permalink)
Young Noob
Join Date: Feb 2011
Posts: 62Rep Power: 9
Davey Richards has alot of potential and to me he is one of the most underrated wrestlers currently in Pro Wrestling and obviously underrated by the IWC. The man has so much talent but it's sad how not alot of people watch his stuff because to me he's awesome.
BQ1: I think he should've but I think it was too soon. I think Strong could've held the ROH World title for 1 more PPV, then give it to Edwards and give him a decent 4-6 month reign.
BQ2: El Generico because that's one more masked man to WWE and he could build a feud with Mistico or Mysterio, depending if Mysterio retires before he arrives.
BQ3: Randy Orton VS CM Punk, this feud has basically been unsolved since Unforgiven 2008, that punt kick is the reason why their feuding and fighting at WM. CM Punk wants his revenge and he wants to beat the hell out of Orton and play mind games with him.
|23 Mar 2011, 05:37 PM
|#4 (permalink)
Fresh Noob
Join Date: Feb 2011
Posts: 43Rep Power: 0
Davey Richards has the most potential of anyone on the ROH roster. I am kind of surprised they haven't had him win the World Title yet.
BQ-1-If you are talking about the TV Tilte I was fine with it. Gave Eddie the chance to transiton from tag to singles.
BQ-2-None ROH is far better than the crap WWE is doing now
BQ-3-None I am not looking forward to Wrestlemania at all the matches are pretty lame and it is going to be another crappy PPV that WWE doesn't care about.
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|12 Apr 2010 02:47 PM


text: Hi people. I have some troubles with win 10 november update. 1. Automatic updates won't give me option to install this major windows 10 update at all. 2. I found manual update solution with MediaCreationTool but when I choose "Upgrade this PC now" option I got message: "The language or the edition of Windows that is currently installed on your PC isn't supported by this tool" I have installed Windows 10 x64 Enterprise edition (build 10240) (VLSC) (English language) and I use Microsoft toolkit to activate windows. Does anyone know what is problem? Thanks!