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Gordon Elliott: Exclusive views on my three runners in the Aintree Grand National
After a hugely successful Cheltenham Festival, quickly followed by his first win in the Irish Grand National, Betfair Ambassador Gordon Elliott has his sights firmly fixed on victory in Saturday's Aintree showpiece...
"He's not an overly big horse but he jumps for fun and has the heart of a lion, he loves a trip - his last two wins have been over 3m6+ and I think he is a perfect type for the National and reuniting his partnership with Davy Russell for the event will suit."
- Gordon Elliott on Tiger Roll
Jack can get a great tune of out stable favourite
17:15 Grand National - Bless The Wings
Bless The Wings is a firm favourite among the staff, he is a real big old fashioned type who is a very careful jumper. He was unlucky at Cheltenham in the Cross-Country race where he slipped and fell at the 12th when going well but aside from that his jumping record is exemplary and he has encountered National fences before, jumping around in the Topham in 2015.
His latest start in the Irish National he just found the ground much too testing, it was near un-raceable and he was never happy so Brian did the sensible thing and pulled him up. His last win came at Cheltenham in December where he stayed on well to come home ahead of Cantlow and favourite Josies Order. Jack Kennedy knows him very well and gets a great tune out of him.
Solid jumping Tiger aiming to keep the success Roll-ing
17:15 Grand National - Tiger Roll
Tiger Roll was incredible in the Cross-Country race at Cheltenham, now a three-time Festival winner he has been a wonderful servant to the yard.
He's not an overly big horse but he jumps for fun and has the heart of a lion, he loves a trip - his last two wins have been over 3m6+ and I think he is a perfect type for the National and reuniting his partnership with Davy Russell for the event will suit.
Obviously this is the Grand National and you need luck on your side but having a good jumper is half the battle and I feel I am very well represented between my three runners, all of whom schooled very well over National fences last week.
Hopeful of a big run for Ucello
17:15 Grand National - Ucello Conti
Ucello Conti was getting a dream run in the National last year when he blundered and unseated Daryl Jacob at the 22nd, the year before he finished fourth of 22 finishers less than 3L behind Vieux Lion Rouge after a great spin around. He is a horse who is ideal for the National and is trained all year with this as his main target.
On his latest appearance in the Thyestes he struggled in very testing conditions but he was second in the race the year before on better ground. Based on his two previous runs in the National I am very hopeful of a big run from the ten-year-old with conditions to suit.
And finally, a nice each way chance in the day's closing race...
18:20 - High Expectations
High Expectations was last seen in November last year, winning a 1m7 Hurdle at Leister on good to soft ground. He has been a great little dual-purpose horse for his owner Martin Wasylocha and we'll continue to have plenty of fun with him. He may have benefited from a run prior to this but he's in excellent form at home and I think he goes here with a nice little each way chance under Donogh Myler.
Betfair's Barry Orr joined pundit Gary O'Brien to preview the Grand National from the Aintree course. Check out our video.


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Happy Anniversary!
2 years
6 months
3 months
February 28th
On this day 2 years ago we pulled into JAARS to begin aviation orientation at 7am the next morning!
We've been in country (PNG) for about 6 months.
We have been in Ukarumpa for 3 months!
Looking back over the past two years I see God's faithfulness.
There were so many times that were so hard we often prayed, "God, is this really what you want for us?"
Each time He answered back yes.
6 months ago we spoke no Tok Pisin!
Now we have our was mama and was papa (our PNG guardian family) coming to visit us here in Ukarumpa.
While they are here we will speak in Tok Pisin exclusively!
Not to say that we are fluent but the fact that we can have conversations and feel somewhat comfortable says a lot by how good our training in country has been. Also how hard we have been trying to keep up with our language skills.
3 months in Ukarumpa.
It's funny; I used to dream about what life would be like when we FINALLY got here!
It often felt like we were coming to the "promise land" in the sense that this was it!
It's where we'd no longer be vagabonds and be able to put down roots.
It's where we'd be able to really dig in and start our ministry.
What I'm finding out is that even here, well especially here that there are many of the same struggles that have always been in everyday life.
What life here has made clear is this:
I need Jesus even more!
I need His grace to fill the gaps,
I need His Spirit to guide me and help me to speak clearly.
I need Him for the same things I've always needed Him for:
Patience when I don't feel like giving it,
Grace when it's not easy to give,
Being able to CHOOSE to show love when I really don't want to!
I'm selfish, you see.
I'm a sinner.
I am no better than you or anyone.
Because I am it makes me very aware that I have a desperate need for Jesus.
I'm reminded of that hymn:
"I need Thee, Oh I need Thee
Every hour I need Thee,
Oh bless me now my Savior,
I come to Thee."
So I am running to Jesus with my joys and sorrows.
My burdens are met by His grace as He covers me and assures me that I am His.
That He loves us.
He is working in our lives.
I can trust Him and His intentions for me AND my family.
So Happy Anniversary to us, and many more to come.
As we journey deeper into daily life with our savior here in PNG and all over the world.
Because we all need Him.

subset: commoncrawl


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The Lincoln Continental has been revived and reinvented since 1939 when the very first Continental was born to Lincoln. After Ford bought out Lincoln during the early 1920s, during times difficulty, and since then has been producing models, with a little bit of the Ford design in mind, to reinvent the line of Lincoln models. The Lincoln Continental was taken off the line after only ten years of production. Ford planned to introduce a Mercury-Lincoln vehicle mix.
In present day we witness Lincoln's introduction, which was long overdue. Some key features include a secure PIN-code keyless-entry system with an E-latch door that opens to reveal a welcome mat with the Lincoln Star Logo, as well as a "spacious" interior with Bridge of Weird Deepsoft leather. You won't have a problem getting comfortable in this car.
Remarkably like the Ford Fusion RS the Continental has a Sync 3 and the AWD system, the Continental comes with comfy Scottish leather, and three different interior designs, Thoroughbred, Rhapsody, and Chalet.
Owners will discover, behind the signature grille, this Continental is equipped with a 305 horsepower 3.7-liter, V6 Engine, and 6-speed transmission. The driver and passengers get to choose their ideal seating position with 30-way power seats with heating and cooling. Optional rear seating package complete with lumbar support and a built-in massage is also a desired feature. This luxury sedan makes room for five, has extra USB ports in the rear seat so everyone can charge their devices. Plus, passengers can experience a small champagne cooler.
Mechanically speaking, the deck has an 8-inch infotainment touchscreen with a 10-speaker audio system to back it (19-speaker Revel is optional), Sync 3 technology, a power sunshade, Premier with parking sensors, rearview cameras, and HID headlights and LED taillights.
The Continental demonstrates a smooth ride and a quiet one in addition to its decent handling. The cabin contains active noise control and acoustic laminated glass, and as an option to absorb road noise, potential owners can add 20-inch foam-lined tires.
Base models start at $45,000 and can go upwards of $80,000 when fully loaded. Visit your local McKie Lincoln Dealer to learn more about a legendary car that has been stealing hearts for decades!


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Born in the USA: Customer Satisfaction and US-based Contact Centers
Remember calling a US-based company only to be connected to an operator someplace else in the world named Peggy?
I do.
According to Claes Fornell International's Contact Center Satisfaction Index 2010, by CEO Sheri Teodoru, many Americans have had lackluster experiences with offshore contact centers, as indicated by collected data:
Teodoru notes that:
"The issue with offshore contact centers continues to be their effectiveness. Customers are far more likely to be shuffled to multiple agents before their issue is resolved, far more likely to have to call multiple times to resolve the issue, and have significantly fewer issues resolved. Not a recipe for success. [Additionally] Callers have a difficult time understanding offshore agents, which leads to an ineffective and inefficient process."
As indicated by the report, contact centers located in the US are better equipped at providing customer satisfaction and solutions.
Voiance Language Services provides language interpretation and translation services, including telephone interpretation, through interpreter contact centers, with over 85,000 square feet of floor space. We manage contact centers, and so do many of our clients, which is part of the Voiance Advantage.


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Get notified when the product is available
Get notified when the product is available
Fragrance description Oriflame Amazing Paradise
Oriflame Amazing Paradise Eau de Parfum is a dynamic yet mysterious fragrance that creates an extremely attractive aura around you.
- a floral fragrance
- citrus fragrance
- a woody fragrance
- for the modern, self-confident woman
- for everyday wear
- Top notes
- Guave, Mandarin Blossom, Violet Leaves
- Middle notes
- Jasmine, Raspberry
- Base notes
- Delonix Regia, Musk, Vanilla
- Fragrance category
- fruity
About the brand Oriflame
The Oriflame company was founded in 1967 in Sweden. Their products, sold through independent beauty consultants, gained quickly in popularity and are now available in more than sixty countries around the world.
The Oriflame beauty range includes hundreds of products containing only the finest ingredients. Oriflame are very particular about their research and employ many top scientists who are constantly working on new and better formulas.
Oriflame's moisturisers, shampoos, shower gels and other products are inspired by the beautiful Swedish nature. That's why they so often include extracts from herbs and plants you can find on Swedish shores and meadows. The same goes for fresh, sensuous and elegant Oriflame fragrances, designed by the most prominent French perfumers.
If animal welfare is your concern, you'll be happy to learn that Oriflame does not test on animals, instead working with human volunteers. That also means that you don't have to worry about adverse reactions and irritation to your skin. An ethical business approach is of utmost importance to Oriflame in general.
Oriflame also supports a number of charitable causes. The company is one of the co-founders of the World Childhood Foundation, an organisation that helps abused and abandoned children.
Oriflame beauty products are sure to satisfy anybody who desires quality at an affordable price.


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Other publications of 1996
Lai,J. & Van der Giessen,E. A numerical analysis of the crack-tip plastic zone in glassy polymers. Petit, J., de Fouquet, J., Henaff, G., Villechaise, P., and Dragon, A. Mechanisms and mechanics of damage and failure. Volume III. 1851-1856. 1996. Warley, Engineering Materials Advisory Services.
Toropov,V.V., Van der Giessen,E. & Yoshida,F. Material parameter identification based on non-trivial experiments, numerical simulation and optimization. Friswell, M. I. and Mottershead, J. E. Identification in engineering systems. 328-337. 1996. Wiltshire, The Cromwell Press.
van der Burg,M.W.D. & Van der Giessen,E. Hydrogen attack in creeping polycrystals due to cavitation on grain boundaries. Thompson, A. W. and Moody, N. R. Hydrogen effects in materials. 313-322. 1996. Warrendale, The Minerals, Metals and Materials Society.
van der Burg,M.W.D., Van der Giessen,E. & Tvergaard,V. Numerical study of hydrogen attack in creeping vessels. Nisitani, H., Aliabadi, M. H., Nishida, S. I., and Cartwright, D. J. Localized damage IV: computer-aided assessment and control. 641-648. 1996. Southampton, Computational Mechanics Publications.
Wu,P.D., Neale,K.W. & Van der Giessen,E. Length changes during large strain reverse torsion. Abe, T. and Tsuta, T. Advances in engineering plasticity and its applications. 229-234. 1996. Amsterdam, Pergamon.
|Last modified:
|22 October 2012 2.29 p.m.


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Tarun Gogoi threatens action against agitationist..
The Chief Minister said the Government was open to giving more powers to autonomous councils. "If they have grievances, we are willing to talk," he stated. The hill districts (Karbi Anglong and Dima Hasao) have been demanding powers given to States like Mizoram including enhanced funding. "I am also sympathetic to their demands and have already conveyed this to the Prime Minister," Gogoi said.full story


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Coffee Roaster 40KG Industrial|840N
840N 40Kg Industrial Coffee Roaster
For industrial purpose.
Exclusive production large batch volume. Create stable quality for your beans.
840N built-in with cyclone、stove window、16 levels of airflow, digital bean and air-flow temp, moreover, adjustable drum speed with stepper motor. Powerful cooling system, it allows you to roast the next batch during cooling process.
Optional ancillary Equipment:Green beans loader、Roasted beans destoner
- Coffee Roaster
- Ancillary Equipment
- Grinder_Industrial
- Grinder_Home / Shop


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Kings ready for 'cool experience' at 2017 China Games
Players taking advantage of perks that come with NHL's first foray into Chinese marketby Nicholas J. Cotsonika and Lisa Dillman / NHL.com Staff Writers
SHANGHAI -- One day last season, Los Angeles Kings defenseman Alec Martinez broke some news to his teammates as one of their NHL Players' Association representatives.
"I remember him saying something like, 'The NHL wants us to go to China,'" goaltender Jeff Zatkoff said. "I think initially everyone was like, 'Man, that's a long ways to go for a couple of games.' But I think once it came out that we were going, everyone was like, 'This is going to be a cool experience.'"
Well, it certainly is a long ways to go for a couple of games.
The Kings took off from L.A. at 2 p.m. PT on Sunday, flew for 13 1/2 hours and 6,500 miles, and landed in Shanghai at 6:30 p.m. local time Monday. Then they cleared customs and took a bus to their hotel.
They play preseason games against the Vancouver Canucks at Mercedes-Benz Arena in Shanghai on Thursday (7:30 a.m. ET; NBCSN, SN, TVA Sports) and Wukesong Arena in Beijing on Saturday (3:30 a.m. ET; NHLN, SN, TVA Sports) in the 2017 China Games presented by O.R.G. Packaging.
But as unusual as this might be for an NHL road trip, it isn't that unusual for the coaches and players. Europeans are used to long travel coming to North America. Many North Americans have played internationally. Lots of players have been on buses in juniors and the minors.
Video: Cotsonika travels to Shanghai with the Kings
"You get much worse horror stories than getting on a nice plane and flying overseas," said Kings coach John Stevens, who remembered taking a 15-hour bus ride from Norfolk, Virginia, to Quebec City once in the American Hockey League, and going from Philadelphia to Cincinnati to Grand Rapids, Michigan, to Milwaukee and back to Philadelphia another time.
"It can be an excuse if you want to use it. So we try to remove that from their options."
The Kings sought advice from a sleep consultant, who told them to put sleep in the bank before the trip, to stay hydrated and not to sleep too much on the plane so they could get their bodies on China's schedule as quickly as possible.
The players had first-class seats and plenty of food to eat. Most brought movies and video games. One brought homework. Zatkoff left Miami (Ohio) University 37 credits short of an accounting degree and has been taking classes so he can graduate. He is taking two while playing right now -- business law and psychology -- and had a textbook in his carry-on bag.
"I think some [teammates] know I'm probably taking classes, but they haven't seen me doing any homework," Zatkoff said. "Usually I do it at home. I'm sure if I bust out the books on the plane, they'll probably look at me like, 'What are you doing?'"
Stevens said the Kings would go for a walk as a group Monday night and sleep in Tuesday morning. He wants two good days of practice and two good games.
"There's lots we need to work on," Steven said. "We know that. Lots we need to improve on, and get other people in the lineup. We're looking for a good week of preparation outside of the fact it's going to be in China."
Video: Gary Bettman on the NHL preseason games in China
But this is more than a couple of games. This is the NHL's first foray into the Chinese market. China is the most populated country in the world, and Shanghai and Beijing are two of the most populated cities in the world. The Kings will conduct meet-and-greets and clinics with young players.
This is also a chance to see a country many of the Kings haven't seen before. They will have free time to explore and plan to visit the Great Wall on Friday.
"It's going to be fun, a lot of fun, and new culture and everything," forward Adrian Kempe said. "You get to see some new things. I think it's going to be really good, and I'm looking forward to it."
In a sense, the trip might not be long enough.
"I mean, who knows if you'll ever get a chance to go back?" Zatkoff said. "So I think now once you kind of get over the travel aspect of it, everyone's excited. I told the guys, 'If I say I'm tired, just smack me and wake me up, because we're only here for a week, so you've got to take advantage of your time over there.'"


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Harnessing Wind
This lesson introduces the ways that engineers study and harness the wind. Students will learn about the different kinds of winds and how to measure wind direction. In addition, students will learn how air pressure creates winds and how engineers build and test wind turbines to harness energy from wind.
Windmill of Your Mind: Distributed Energy Goes to School
Students research the feasibility of installing a wind-turbine distributed energy (DE) system for their school. They write a proposal (actually, the executive summary of a proposal) to the school principal based on their findings and recommendations. While this activity is geared towards fifth-grade and older students, and Internet research capabilities are required, some portions of this activity may be appropriate for younger students.
Is That Legal? A Case of Acid Rain
The goal of this activity is to understand how techniques of persuasion (including background, supporting evidence, storytelling and the call to action) are used to develop an argument for or against a topic. Students develop an environmental case study for presentation and understand how a case study is used as an analysis tool.
Is That Legal? A Case of Acid Rain
The goal of this activity is to understand how techniques of persuasion (including background, supporting evidence, storytelling and the call to action) are used to develop an argument for or against a topic. Students develop an environmental case study for presentation and understand how a case study is used as an analysis tool.
Green Marketing
Students learn basic marketing concepts and use professional marketing techniques to compose an advertisement for a hybrid vehicle. In the process, they learn the principles of comparative analysis.
Sructured Electronic Design
This course focuses on a systematic approach to the design of analog electronic circuits. The methodology presented in the course is based on the concepts of hierarchy, orthogonality and efficient modeling. It is applied to the design of negative-feedback amplifiers. It is shown that aspects such as ideal transfer; noise performance, distortion and bandwidth can be optimized independently. A systematic approach to biasing completes the discussion. Lectures are interactive and combined with weekl
Stochastic Evolution Equations
The lectures are at a beginning graduate level and assume only basic familiarity with Functional Analysis and Probability Theory. Topics covered include: Random variables in Banach spaces: Gaussian random variables, contraction principles, Kahane-Khintchine inequality, Anderson's inequality. Stochastic integration in Banach spaces I: γ-Radonifying operators, γ-boundedness, Brownian motion, Wiener stochastic integral. Stochastic evolution equations I: Linear stochastic evolution equations
Mathematical analysis
This is a module framework. It can be viewed online or downloaded as a zip file. As taught in 2007-2008 and 2009-2010. This module introduces mathematical analysis building upon the experience of limits of sequences and properties of real numbers and on calculus. It includes limits and continuity of functions between Euclidean spaces, differentiation and integration. A variety of very important new concepts are introduced by investigating the properties of numerous examples, and developing t
The legacy of the slave trade
On the 200th anniversary of the abolition of the slave trade Cecily Jones discusses the legacy of the trade and why an apology is necessary. Length: 18 minutes
Women's fight for equal pay
The launch of the equal pay archive at Warwicks Modern Records Centre is discussed by three figures who have played a central role in the battle for equal pay for women. They are Jo Morris, senior equality and employment rights officer of the Trades Union Congress, Sue Hastings, an independent pay and employment adviser, along with Rodney Bickerstaffe, the former general secretary of the public sector union Unison and chairman of the Modern Records Centres Advisory Board.
Policy Analysis of Multi-Actor Systems
This course is about solving complex problems. Our favorite problems are not just technically complex but also characterized by the presence of many different social actors that hold conflicting interests, objectives, and perceptions and act strategically to get the best out of a problem situation. This course offers guidance for policy analysts who want to assess if and how their analysis could be of help, based on the premise that problem formulation is the cornerstone in addressing complex pr
6.047 Computational Biology: Genomes, Networks, Evolution (MIT)
This course focuses on the algorithmic and machine learning foundations of computational biology, combining theory with practice. We study the principles of algorithm design for biological datasets, and analyze influential problems and techniques. We use these to analyze real datasets from large-scale studies in genomics and proteomics. The topics covered include: Genomes: biological sequence analysis, hidden Markov models, gene finding, RNA folding, sequence alignment, genome assembly Networks
11.304J Site and Infrastructure Systems Planning (MIT)
This course is a client-based land analysis and site planning project. The primary focus of the course changes from year to year. This year the focus is on Japan's New Towns. Students will review land inventory, analysis, and planning of sites and the infrastructure systems that serve them. They will also examine spatial organization of uses, parcelization, design of roadways, grading, utility systems, stormwater runoff, parking, traffic and off-site impacts, as well as landscaping. Le
6.012 Microelectronic Devices and Circuits (MIT)
6.012 is the header course for the department's "Devices, Circuits and Systems" concentration. The topics covered include modeling of microelectronic devices, basic microelectronic circuit analysis and design, physical electronics of semiconductor junction and MOS devices, relation of electrical behavior to internal physical processes, development of circuit models, and understanding the uses and limitations of various models. The course uses incremental and large-signal techniques to analyze an
14.121 Microeconomic Theory I (MIT)
This half-semester course provides an introduction to microeconomic theory designed to meet the needs of students in the economics Ph.D. program. Some parts of the course are designed to teach material that all graduate students should know. Others are used to introduce methodologies. Topics include consumer and producer theory, markets and competition, general equilibrium, and tools of comparative statics and their application to price theory. Some topics of recent interest may also be covered.
6.172 Performance Engineering of Software Systems (MIT)
Modern computing platforms provide unprecedented amounts of raw computational power. But significant complexity comes along with this power, to the point that making useful computations exploit even a fraction of the potential of the computing platform is a substantial challenge. Indeed, obtaining good performance requires a comprehensive understanding of all layers of the underlying platform, deep insight into the computation at hand, and the ingenuity and creativity required to obtain an effec


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YALLFest is coming! YALLFest is coming! On November 9th, 2013, fifty YA authors will descend on Charleston, SC, for an amazing day of free panels, presentations, and book signings. Visit the YALLFest website for more details. In the weeks leading up to the big event, I'm hosting interviews with the talented YALLFest authors several times a week, so keep stopping by to learn more about these fabulous folks.
Today's featured author is Lauren Oliver, a New York born and bred gal, whose books have not only been New York Times bestsellers but have also been on just about every other noteworthy list as well. Seriously, way too many accolades to mention in one blog post. Her YA books include Before I Fall and the Delirium trilogy (as well as other stories set in the world of Delirium), and she's also written books for younger readers like Liesl & Po and The Spindlers.
And now for the Q&A with Lauren:
What one thing do you need to have when you write?
What is the hardest line to write- the first or the last?
Honestly, neither. It's just every single line in between that feels impossible.
Best writing tip you ever received?
Aim for truth and beauty will follow.
Tell us 5 random facts about yourself.
I have fourteen tattoos. I can fall asleep any time, any place. I have a phobia of worms. I hate fruit. I wrote my first novel largely on my Blackberry.
Where's your favorite place to write?
In my sunroom in upstate New York.
What are you working on now?
Two different middle grade books and an untitled young adult novel that my agent described as "the most disturbing thing he's ever read." Oops!
What is your favorite genre to write in? To Read?
I love realistic books that include just a hint of fantasy.
Are you planning to attend YALLFest? Have you read any of Lauren's books? Which one(s) are your favorite(s)?
Previous interviews:


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General Information: Also known as purple-leaf basil, is a variety of the more usual green basil. This variety has dark, purply-red leaves, stems and flowers and is often grown as a decorative garden plant. Like other varieties of basil, opal basil thrives in warm, sunny climates and can be damaged by frost.
Opal basil has the same distinctive clove like smell as green basil
Recipes: 0


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Off Season Hours 11:30-9:00 PM & Sunday 10:00 A
Fanizzi's By the Sea
Fanizzi's Restaurant
539 Commercial St., Provincetown
(508) 487-1964
Open Year Round and Daily
Closed Christmas and Thanksgiving Day
Just outside the midtown bustle of Commercial Street lies Fanizzi's By the Sea in a picturesque, sea-weathered house. With one of the most spectacular views of Cape Cod Bay in town, enjoy the window-lined room, as the salt sprays only feet from your table. Or, enjoy a drink and a comfort food classic in the cozy bar. Owner Paul Fanizzi and Chef Don Lloyd have mastered the art of the eclectic menu, offering everything from Cape seafood classics to fresh salads, elegant, international entrées to burgers. Open daily and year-round. Sunday Brunch 10:00 AM - 2:00 PM; lunch menu served from 11:30 AM - 3:45 PM and Dinner served 4:00 PM – Close. Early Birds Special 4:30-6 p.m. Function room available. Parking Available. Visit us on face book. www.fanizzisrestaurant.com.
Fanizzi's By the Sea


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Quality Care Medical Clinic
Family Practice and Internal Medicine in Yakima Washington
Dr. Lyzanchuk is a Board Certified Family Practice Doctor. He has been practicing in Yakima over 25 years. He has patients that range from 1 month to 90 years old. He is great with children.
Quality Care Medical Clinic
611 N. 39th Avenue
Yakima, WA 98902
Phone: (509) 249-1288
Fax: (509) 249-6249
Monday-Thursday: 7am – 5pm
Friday: 7am – 1pm
[Get Directions...]
Family Practice Physician
Full Time
Salary & Incentives
Health Benefits & Malpractice Paid
Call our office at (509) 249-1288 for details.
View our Privacy Practices (pdf).
© Copyright 2012 Quality Care Medical Clinic · All Rights Reserved · Website by Jason Larsen


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In November of 2013, there was an event that took place at San Jose State University. Donald Williams Jr, an African American, was a 17 year old freshman at the university when he was brutally bullied by four white students.The four white students bullied Donald multiple times by abusing him, including wrestling him to the ground, fastening a bike lock around his neck, calling him racially derogatory names, locking him in his room and displaying a Confederate flag in their suite. Donald's parents came down for the weekend and saw the confederate flag hanging on a bulletin board and immediately filed a report to the university. Once the university got notice about this event, they took immediate action and moved the students into a different living situation starting the investigation. San Jose State made the decision to expel three of the students and one student was put on suspension because he was seen more as a bystander. The state of California was also involved and decided that the three expelled students cannot enroll in any California State University college in the future.
Once the University started investigating this event it was only moments later when it became very popular to outside viewers on social media. Because of the racist actions done to the student and the pending consequences for the four students participating everyone took their stance on this event. This event sparked community outrage within San Jose State University as well as on the internet through news articles, Twitter, YouTube and Facebook. Though social media, many people were able to state what they believed should be done to the students, make comments and even make more racist comments about this whole situation. Based on the social media articles, there is colorblindness demonstrated by online users about the event taken place at San Jose State University.
Continue reading Is San Jose State University Really Accepting of Its Wide Diversity?


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Dutch national wealth is measured by the Gross domestic product (GDP). Economics and hard data. But the wealth of prosperity of a nation is more than only money; it is also related to more soft (subjective or emotional), less tangible data. Think of variables as: environment, health, social cohesion, fear, safety, innovation, good quality of life, education and equal opportunities.
What the indicators of this broader notion of prosperity are and how they can be measured is not clear yet.


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Job Description
Company Profile: Our client is Japanese machinery for factory company. They are looking for candidate to join as an Import-Export document control officer
Job Summary:
- Review Transaction Screening Sheet for SD & TSD.
- Coordinate, follow up and complete all relevant with Transaction Screening Sheet from aboard.
- Support job related with Compliance Dept.
- Prepare presentation and minute of meeting upon request.
- Female only, not over 27 years old, Thai Nationality
- Bachelor's Degree in Business Administration,Business English, International Business.
- At least 1 years experiences for Import-Export field is advantage.
- Fresh graduate are welcome!!!!
- Experienced in trading machinery company is a plus.
- Good communicate in English (TOEIC Score 550 UP).
Location: Chaengwattana Road, Bangkaen, Bangkok
To apply for this job, click on the apply button or contact the consultant above directly.
(*Only shortlisted candidate will be contacted.)


text: We've just found out I will need to have a hysteroscopy to investigate a polyp found during our NHS IVF cycle in March. I was hoping we could begin treatment for our FET on my next cycle in June but now it will be July at the earliest (if our appointment for the hysteroscopy comes through in June). I'm 41 and feel like time is running out - feeling frustrated 😤


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|Citing eHRAF
If you have used HRAF's subject-indexing system in an Advanced Search, we suggest identifying the Subject Description and the OCM identifier from the Outline of Cultural Materials (OCM).
Ember, Carol R., and Melvin Ember. 2005. Explaining Corporal Punishment of Children: A Cross‐Cultural Study. American Anthropologist 107(4): 609-619.
Gray, Peter B., and Sharon M. Young. 2011. Human–Pet Dynamics in Cross-Cultural Perspective. Anthrozoos: A Multidisciplinary Journal of The Interactions of People & Animals 24(1): 17-30.
Murdock, George P., Clellan S. Ford, Alfred E. Hudson, Raymond Kennedy, Leo W. Simmons, John W. M. Whiting. 2008. Outline of Cultural Materials. 6th revised edition with modifications. New Haven: Human Relations Area Files.
Murdock, George P., and Douglas R. White. 1969. Standard cross-cultural sample. Ethnology. 329-369.
White, Douglas R. 1989. Focused ethnographic bibliography: Standard cross-cultural sample. Cross-Cultural Research 23(1-4): 1-145.


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For those of you that know what I've been doing for the last few months… I can let you know that I'm "pau for nau".
We have one more operation that will begin in July… after that operation… I'll be "pau for gaud".
It's been a nice little gig of work… definitely interesting. Some of my co-workers have called this a "Sociology Experiment being done to see how far they can push people".
I guess folks can push me pretty far before I break… as I'm just pau for now.
Filed under: Big Island, Community, National Affairs, State Affairs Tagged: | Pau, Working for the Government


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No More Dental Implants, Grow Teeth by Yourself in Only 9 Weeks
Doctor Jeremy Mao and his team of scientists from the Colombian University came up with the wildest futuristic discovery. According to them, there is...Read more »
How to Grow an Unlimited Amount of Blueberries in Your Backyard!
You should definitely include blueberries in your diet as they are rich in nutrients and antioxidants. Lately, it has been shown that blueberries help...Read more »
If You See This "Weed" Growing In Your Yard, Don't Pick It! Here's Why…
Are you one of those who think purslane is just a useless weed that grows by itself in the yard? Well, here is a...Read more »
How To Grow Your Own Avocado Tree In Small Garden Pot
One of the best and sweetest summer fruits is avocado. You probably have wanted to have it in unlimited amounts, so, next time you...Read more »
Take This 2-Ingredient Sleep Recipe Before Bed To Never Wake Up Groggy Again
Everybody is aware of the importance of getting a good night's sleep. However, over the past few years, the average of sleeping hours has...Read more »


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Monday, August 25, 2008
Herb roasted pork chops and vegetables
Prep Time: 15 minutes
Start to finish: 55 minutes
3/4 teaspoon Italian seasoning
1/2 teaspoon salt
1/4 teaspoon pepper
8 small new red potatoes, unpeeled, quartered (about 1 lb)
2 cups ready-to-eat baby-cut carrots
1 medium onion, cut into 8 wedges
1 large clove garlic, finely chopped
1 tablespoon olive oil
4 boneless pork loin chops (4 oz each), trimmed of fat
1. Heat oven to 450 °F. Spray 15x10x1-inch pan with cooking spray. In small bowl, mix Italian seasoning, salt and pepper.
2. In large bowl, mix potatoes, carrots, onion and garlic. Sprinkle with oil and half of herb mixture; toss to coat evenly. Place in center of pan.
3. Bake 20 minutes; stir vegetables. Sprinkle remaining herb mixture over pork chops. Place pork chops around vegetables. Bake 15 - 20 minutes or until pork is no longer pink and meat thermometer inserted in center reads 160°F, and vegetables are tender.
Exchanges: 1/2 starch, 1 vegetable, 3 lean meat, 1/2 fat
Carbohydrates choices: 2
2 comments:
- Beachlover said...
now you make me wanna to roasted pork chop tomorrow.
- August 25, 2008 at 9:25 PM
- Puteri said...
Yeah, been a while since I had pork chops too! That was good!
- August 26, 2008 at 9:35 AM
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On the day of the seminar, your name will be listed on the roster; it is not necessary to bring any paperwork. You may wish to bring a pen and paper for notes. Please consider layered clothing as heating and cooling conditions may vary.
What do I need to bring with me to the seminar?
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Continuing their support of Isle of Wight Pride, Wightlink are proud to announce their amazing Early Bird travel offers to keep the crossing to the Isle of Wight Pride as low as possible.
Earlybird discounts if you travel to UKPRIDE2018 with Wightlink!
1. This is a time limited offer which can only be booked online using the link – accessed via http://www.wightlink.co.uk/best-events/isle-of-wight-pride/
2. Early bird foot passenger fares are valid for bookings made between 1 February 2018 and 14 July 2018 or until limit has been reached.
3. Day Return and Short Stay travel must take place as specified from the Mainland to the Island (Isle of Wight) on our Portsmouth Harbour to Ryde Pier Head FastCat route.
4. We reserve the right to modify or cancel the offer at any time.
5. No adjustments can be made on previous purchases.
6. Offer cannot be used against Season Tickets, Multilink Passes or any promotional offers including Tesco Clubcard Boost tokens, already discounted fares and B2B (business to business) bookings.
7. Wightlink General Conditions for provision of Ferry Services apply and can be found at www.wightlink.co.uk/terms.


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Final settlement reached in Stronelairg campaign
Trust reaches settlement with Scottish and Southern Energy (SSE) in Stronelairg wind farm legal case
The John Muir Trust has agreed to pay Scottish and Southern Energy (SSE) £50,000 in final settlement of court costs for the Stronelairg legal case, bringing our campaign against the Stronelairg wind farm development to a conclusion.
The settlement is significantly less than the £350,000 which SSE was pursuing through the court system.
This agreement, combined with the £75,000 agreed with the Scottish Government last month, ends our liability for the Stronelairg legal case. The Trust is extremely grateful to our legal team who supported us throughout and minimised their costs where possible.
This is a positive outcome, and we are grateful to the many supporters of our Stronelairg campaign, who have enabled us to meet all of the legal costs from our existing campaign funds.
Next steps in our Wild Land Campaign
With your support, we will continue to work on the next stages of our Wild Land Campaign. Looking ahead, we see several challenges emerging, especially in the far north of Scotland. These include the Caplich windfarm, near Assynt, which goes to Public Local Inquiry in June and the re-submitted Limekiln windfarm, in west Caithness, which is likely to go to Inquiry.
Our campaign will focus on obtaining expert advice, attending Public Local Inquiries, and delivering reports and consultation responses to influence government policies.
The Trust believes in a more positive vision for this remote and beautiful area, and is actively supporting the campaign for World Heritage Status for the Caithness and Sutherland Flow Country peatlands.
Read more about our work to protect wild land
Support our work with a donation


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Fotolog acquired
This is old news by now, but photo-sharing site Fotolog, especially popular in Latin America, has been acquired by Hi-Media Group for a reported $90 million.
More info at:
So much for that idea
So much for taking the Contax 159MM out for a spin. The shutter appears to be stuck, so I'll have to take it in for repairs.
I've loaded up the Nikon FG instead.
Shadow Play
Liz Ashley, July 2007
(Purchase a print of this photo | View this photo on Flickr)
The FSLR is dead, long live the FSLR
While writing yesterday's post about the new DSLRs from Canon and Nikon, I started thinking about the DSLR label. Digital SLR. Why do we even bother with the "D" anymore? Digital has won. Sure, there are still a few film SLRs being manufactured, but they are largely ignored, at least in the 35mm world. They are the odd balls. They should be FSLRs.
So starting right now, on this blog, digital SLRs shall be known as SLRs and film SLRs as FSLRs.
I'm going to take my Contax 159MM, one of my favorite FSLRs of all time, and a roll of film out for a spin. I'll post some pics in a couple weeks after the film is developed. Long live the FSLR!
Now go out and shoot.
New cameras galore
If you were living under a rock this week, or if you were too busy watching your net worth plummet on Google Finance, you might have missed the new camera announcements.
Canon announced two new DSLRs and a new compact:
- The Canon EOS 40D 10MP APS-C DSLR
- The Canon EOS 1Ds Mark III 21MP (!!) Full Frame DSLR
- The nifty Canon PowerShot G9 12MP compact point & shoot
Nikon followed shortly thereafter, announcing two new DSLRs of their own:
- The Nikon D300 12MP APS-C DSLR
- The Nikon D3 12MP Full Frame DSLR
Good stuff. Did I mention that the EOS 1Ds Mark III 21MP?!? Canon finally made a medium format camera.
And welcome to full-frame, Nikon users! Nikon's entry into the full-frame market should be exciting news for Nikon and Canon fans because the competition will lead to lower-priced full-frame DSLRs in the near future.
Oh, and in case you're wondering, I'm not going to buy any of them. I wouldn't even if my net worth hadn't dropped like 50% the last couple weeks. I won't need more than my 5D for a long, long time. If I were going to buy one, it would probably be the G9.
Halter Ranch Vineyard, Paso Robles, California
Halter Ranch Vineyard, Paso Robles, July 2007
(View on Flickr)


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Sound Clip Saturday: Back Over There
April 6, 2017 marked the 100th anniversary of the United States' entry into World War One—a war in which millions of Americans fought and more than 50,000 died in combat. In The Last of the Doughboys, author Richard Rubin introduced listeners to this largely forgotten generation of Americans. Rubins interviewed the war's last survivors face-to-face and knew well the importance of being present if you want to get the real story. But he soon came to realize that to get the whole story, he had to go Over There, too. So he did… and discovered that while most Americans regard that war as dead and gone, to the French, who still live among its ruins and memories, WWI remains very much alive.
Years later, Rubin decided to go back Over There to follow the trail of the American Expeditionary Forces on the Western Front, finding trenches, tunnels, bunkers, century-old graffiti and ubiquitous artifacts. But what he wasn't expecting to find was an abiding fondness for America and Americans, and a colorful corps of local after-hours historians and archeologists who tirelessly explore these sites and preserve the memories they embody while patiently waiting for Americans to return and reclaim their own history and heritage.
Based on his wildly popular New York Times series, Richard Rubins' Back Over There audiobook is a timely journey, in turns reverent and iconoclastic but always fascinating, through a place where the past and present are never really separated. Listen to an excerpt of the Back Over There audiobook, read for you by the author:
Back Over There is now available as an unabridged CD and digital download: Amazon, Barnes & Noble, Audible, Audiobooks.com, IndieBound, Libro.fm


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Perspective ARTICLE
Cre-Lox Neurogenetics: 20 Years of Versatile Applications in Brain Research and Counting…
- Brain and Behavior Discovery Institute, Medical College of Georgia, Augusta University, Augusta, GA, USA
Defining and manipulating specific neurons in the brain has garnered enormous interest in recent years, because such an approach is now widely recognized as crucial for deepening our understanding of how the brain works. When I started exploring the Cre-loxP recombination for brain research in the early 1990s, it was written off as a dead-end project by a young fool. Yet over the past 20 years, Cre-lox recombination-mediated neurogenetics has emerged as one of the most powerful and versatile technology platforms for cell-specific gene knockouts, transgenic overexpression, Brainbow imaging, neural pathway tracing with retrovirus and CLARITY, chemical genetics, and optogenetics. Its popularity and greater utility in neuroscience research is also largely thanks to the NIH's bold Blueprint for Neuroscience Research Initiative to launch several Cre-driver resource projects, as well as individual laboratories and private research organizations. With newly-discovered, genetically-encoded molecules that are capable of responding to sonar and magnetic stimulation, for sonogenetics or magnetogenetics, respectively, or detecting rapid voltage changes in neurons, Cre-lox neurogenetics will continue to aid brain research for years to come.
Santiago Ramón y Cajal was among the first to observe and marvel at the elegance and beauty of various neurons in the brain and wonder how they contribute to perception, emotion, memory, and behavior (Cajal, 1909/1910). Structure-functional analysis of neurons began in earnest with the development of single-cell, juxtacellular-labeling, and patch-clamp techniques (Jankowska et al., 1976; McCrea et al., 1976; Hamill et al., 1981; Edwards et al., 1989; Pinault, 1996; Klausberger and Somogyi, 2008). Various other technologies have further transformed the neuroscience landscape at multiple levels – ranging from genes and proteins (Carraway and Leeman, 1973; Hökfelt et al., 1977; Noda et al., 1984; Seeburg et al., 1990; Buck and Axel, 1991; Moriyoshi et al., 1991; Monyer et al., 1992; Carlsson, 1993; Südhof, 2012), to synaptic plasticity and cognitive enhancement (Tang et al., 1999; Lømo, 2003; Frey and Frey, 2008; Wang et al., 2009).
In the late 1980s and early 1990s, following the pioneering efforts by Mario Capecchi, Oliver Smithies, and Martin Evans on gene targeting and embryonic stem cell (ES) technologies (see review by Capecchi, 2005), a small number of laboratories began to generate mutant mice to define genes' function in development, cancer, or immunology (Zijlstra et al., 1989; DeChiara et al., 1990; Koller et al., 1990; Thomas and Capecchi, 1990). Alcino Silva, Seth Grant, and Thomas O'Dell were among the first to apply the gene knockout approach to study the specific roles of CaMKII or Fyn kinase in plasticity and memory (Grant et al., 1992; Silva et al., 1992a,b). These studies set a new stage to investigate genes and brain functions. However, the limitations such as the lack of phenotypes due to genetic compensation or the developmental effects were noticed. For example, increased spontaneous epilepsy was observed in the CaMKII knockout mice, whereas the dentate gyrus was deformed in the Fyn knockout mice. Both cases led to heated debate in the field about how the reported memory and plasticity deficits should be interpreted. With the NMDA receptor occupying a center stage in plasticity and memory field, Li et al. (1994) set out to demonstrate its role in learning behavior, but they found that NMDAR1 knockout pups all died neonatally due to failed brain development, including the defective suckling reflex. For developmental neuroscientists, it was very exciting, because stereotypic whisker barrel in the thalamus failed to form (Li et al., 1994). Yet for cognitive and behavioral neuroscientists, it was disappointing. It further solidified the argument that deleting the gene in every cell and organ throughout development was not ideal for defining cognitive mechanisms.
Young and Foolish
My interest in developing the conditional gene knockout technique stemmed from the specific problem at my hand as I was finalizing my HHMI postdoctoral fellowship with Eric Kandel at Columbia University in the summer of 1993. From 1990 to 1993, I was testing the long-held idea, first postulated by Bernie Agranoff (Agranoff and Klinger, 1964), that long-term memory requires new protein synthesis and gene expression. Researchers were racing to identify novel genes whose expressions were regulated by brain activity. We were the first to pull out a set of genes which included tissue-plasminogen activator (tPA), a MAP kinase phosphatase, and a brain-specific immediate early gene BAD1 (published under my former name, Qian et al., 1993, 1994). BAD1 was later also isolated by Paul Worley and named as Arc (Lyford et al., 1995). While isolating these novel genes was exciting, the next logical step for me was to examine their functions in memory. Anti-sense oligonucleotide-based "knock-down" methods were imprecise and not reliable, whereas gene-knockout was a good choice, but its limitations and caveats were obvious.
I stumbled upon a paper by Brian Sauer on his successful demonstration of the Cre recombinase-based excision of a floxed marker gene from the circular plasmid transfected in mammalian culture cells (Sauer and Henderson, 1988). The last paragraph of this paper put forth a big question: "Can Cre also cause recombination at lox sites located within the genome of a mammalian cell?" This led me to wonder if I could use it to develop brain subregion- and cell type-specific gene knockout and/or transgenic overexpression methods. But I knew that all the textbooks and literature describing DNA replication and recombination go hand in hand with cells division (such as meiosis or mitosis; Figure 1). This basic doctrine was imprinted into everyone's mind, and also evident from the opening sentences of Sauer's paper: "The processes governing DNA recombination in mitotic mammalian cells have been the subject of intense investigation in recent years… Mitotic recombination plays a central role in the development and function of the immune system." Knowing that all neurons in the adult brain are known to be postmitotic right after birth (except a few in the dentate gyrus and olfactory bulb), any fool who set out to work on DNA recombination in the adult brain would be committing a career suicide. Nature has shown that brain tumors are all in glial cells which divide, but not in neurons. Therefore, DNA recombination as the way to create region- and cell type-specific knockout in the brain was considered to be plainly impossible. I was told that the next show was not about how and why I would fail, but where.
FIGURE 1. DNA recombination involves complex topological and biochemical during DNA replication. DNA recombination is linked with DNA replication during cell divisions in order to exchange genetic materials for the selection of favorable mutations or the elimination of unfavorable or deleterious mutations. Recombination also comes with DNA injury and is therefore tightly coupled with the DNA repair mechanism. Nearly all neurons in the adult brain are post-mitotic to ensure their stability. This explains why brain tumors are in glial cells but not in neurons.
But I was obsessed with the thought of wanting of know the function of genes in a clear way, and only a handful of laboratories had gene-targeting facilities and embryonic stem (ES) cells for making conventional knockout mice. I asked Mario Capecchi of Utah and Susumu Tonegawa of MIT for a second postdoc position, and both said yes. When I sought advice, to my great surprise, Eric suggested that I should choose Susumu's lab as it would be a better place for trying my idea. I also got the permission to use the CaMKII promoter, cloned by Mark Mayford, which becomes active after the second or third postnatal week and only expresses the forebrain principal cells such as pyramidal cells (Mayford et al., 2005). The stage was all set, with the only minor bug: if my idea did not work out at this second postdoc stage, I would be out of a job.
When I arrived at MIT in the fall of 1993, I was pleased to find that Susumu did not discourage me from exploring Cre-loxP neurogenetics during our 15-s introduction meeting. I was surprised by the zoo-like atmosphere in this 40 postdoctorals/students laboratory. Miraculously, this survival-of-the-fittest model worked well for productivity, and overall MIT was exciting and refreshing to me.
To develop Cre-loxP-neurogenetics, I must ignore three risks: (1) to hope the textbook on DNA recombination linked with replication was wrong, and my argument was that herpes virus (producing cold sore) infected the peripheral nerve ganglia and somehow replicated itself. (The mechanism still remains unknown to date, but presumably without ganglia neuron division.) (2) The complex procedures and long cycles with no quick feedback and no room for error. I must make various constructs and generate at least three different mouse lines and then embark on a multi-year crossing to breed them together (Figures 2A,B). Back then, making even one mutant mouse was already a form of art and luck. In fact, only a few brave souls in well-equipped laboratories could do so because of the complex procedures, lengthy project cycle, expensive cost, and with potentially no phenotypes or undesirable outcomes at the end. A few lucky ones got good jobs and the other unlucky ones simply disappeared. (3) The intentional risk which I brought upon myself: I chose to work on the NMDAR1 as the gene for conditional knockout, instead of the BAD1/Arc. As everyone knew, if the sites to which I inserted the LoxP somehow disrupted its expression, I would have a dead pup that was to be published in a few months by Li et al. (1994). In contrast, unintentional disruption of Arc from the loxP insertion would still give me a conventional knockout paper. But I convinced myself the idea was promising, and my gamble would pay off: I would, at least, get a badge of honor for being the first fool to throw myself under the bus of the textbook dogma.
FIGURE 2. Cre-LoxP neurogenetics for achieving region- and cell-type specific analysis of relationships of genes, circuits, and functions in the brain. (A) Strategy to detect the Cre-LoxP recombination in the brain. It required the production of two different transgenic mice: Tg-Cre and Tg-Reporter lines, respectively. These two lines were then crossed to generate the double transgenic mice. CA1-specific recombination was detected after P19 by LacZ method. It is known that CA1 pyramidal cells undergo neurogenesis between E10 and E18 and enter the post-mitotic state by P0. They are well differentiated by P7, with fully established synaptic connections. We have found that Cre-loxP recombination occurs during the middle or end of the third postnatal week in the CA1 pyramidal cells. LacZ in the CA1 pyramidal cells was detected as deep blue color on Nissl stain (in purple-blue) background. (B) Conditional knockout of NR1 gene in a specific cell type and region. The exon 11-21 encoding the entire transmembrane domain and C-terminus were flanked by the loxP (termed floxed) in the introns. The second loxP was followed with the neo cassette gene, which allowed for targeted ES cells selection. Luckily, insertion of loxP sites and the neo gene did not alter NR1 gene expression in the floxed homozygous mice.
The execution required complex choreography. Despite all of the chaos in the new laboratory, I found friendly colleagues willing to help me. There was David Gerber and Toshikuni Sasaoka who taught me about microinjections in fertilized eggs and blastocysts, respectively; Dongfeng Chen who helped with immune-antibody and staining; Yuqing Li who shared his insights into the NMDAR1 constructs; Min Xu who provided me ES cells and guided me through delicate ES cell-culture procedures; I was also grateful to David Anderson at CalTech for the LacZ reporter and Brian Saucer at Du Pont for Cre-loxP plasmids. I completed all my constructs by January of 1994 and started production of transgenic mice and ES cell targeting of the floxed NR1 construct. I also had a hard-working undergraduate student named Cindy Tom and technician Jason Derwon, who assisted with the genotyping and brain sectioning.
One ominous cloud moved over my head when Klaus Rajewsky reported in July 1994 that they achieved 50% knocked down of the RNA polymerase in T cells (Gu et al., 1994). This meant that even in dividing T cells, Cre-recombination was not efficient: either 50% of the T cells had 100% knockout of RNA polymerase or 100% of the cells had only one copy of the gene deleted, or worse, a mixed mosaic situation. Despite this, I held out the hope (after additional digging into the literature) that the transiently active promoter they used might be the culprit for the poor efficiency during T cell development. Over the ensuing months and years, I stuck my head in the sand and labored over my Cre-loxP neurogenetics experiments. In the late fall of 1994, I finally got the first feedback from the experiments.
On a sunny but cold morning, I recall the great surprise when I saw the intense LacZ staining, specifically in the CA1 pyramidal cells of the hippocampus in the first Cre transgenic line (Figure 2). I could not believe my incredible luck, because the CA1 hippocampal region was the center of the universe in the eyes of many plasticity and memory researchers. Additional Cre lines confirmed similar CA1-specific recombination; then other Cre lines showed forebrain-specific patterns. When Susumu returned from a trip to Japan, I told him about what I had found. Once he recovered from his confusion, which seemed to result from jetlag and attempting to grasp all that I had been working on, he overcame his disbelief and immediately shared his jubilation with Alcino over the phone. I asked myself why the CaMKII promoter, which was supposed to express in the forebrain – not specific to the CA1- had such a specific effect. Dongfeng Chen helped provide the clue by revealing that Cre expression was higher in the CA1 pyramidal cells. Over the following 6–8 months, I also obtained the floxed NR1 homozygous mice crossed with the Cre Tg 29.1 line. I confirmed the CA1 pyramidal cell-specific NMDA receptor knockout. Pato Huerta and I proceeded with brain slice recordings or histology, respectively, as soon as we could obtain young adult mice (∼3–5 weeks). We also set aside a set of mice for collaborating with Tom McHugh, Kenny Blum, and Matthew Wilson for place cell recordings in the NR1 mutants. Now, we know that the Cre-lox recombination in T29-1 retained the CA1 specificity only in an age-dependent manner: as Cre expression accumulated over time (starting in the 6th–8th postnatal weeks), it would gradually achieve the forebrain-specific recombination (as expected from the CaMKII promoter).
By the fall of 1996, we were preparing three back-to-back manuscripts to be submitted to Cell. We had decided that reporting the feasibility of the Cre-lox neurogenetics would be the first, the CA1-specific NMDA receptor knockout study the second, and the place-cell characterization paper would be published as the third. The original agreement with Eric for using the CaMKII promotor was to include him and Mark as the co-authors in the first paper, in case the Cre-lox work progressed to a point of publication. This initially doomed project now unexpectedly resulted in multiple results, which created its own political nuisance. Knowing the immense interest of the CA1-specific NMDA receptor knockouts in the learning and memory field, Eric preferred his co-authorship be placed in the second paper, not the first Methodology paper. But Susumu insisted that Eric's name be in the first paper. The back-and-forth conversations that ensued were very distressful to me, but allowed my brain to form an unusually strong long-term semantic memory of what it means to be caught between a rock and a hard place. The papers were eventually published in December 1996 (McHugh et al., 1996; Tsien et al., 1996a,b), but they were left with no corresponding author.
Cre-Driver Resources for Neurosciences
The successful demonstration of the feasibility of Cre-lox neurogenectis has generated a firestorm in the field. Recognizing its unique usefulness for neuroscience, the NIH launched the NIH Blueprint for Neuroscience Research, known as the Cre-driver project, to create a collection of mouse strains for better defining the functions of specific cell types and neural circuits in cognitive behaviors1. I was glad to provide my inputs during the process. The NIH chose three centers in the United States for the generation of genetically modified mice expressing Cre recombinases in the nervous system. The teams were led by Dr. Ronald Davis at Baylor College of Medicine (now at Scripps Research Institute-Florida), Dr. Josh Huang at Cold Spring Harbor Laboratory (co-led with Sacha Nelson of Brandeis University) and Dr. Ulrich Mueller at Scripps Research Institute. This program generated more than hundreds of Cre-driver lines (Taniguchi et al., 2011). The mice are available through the Mutant Mouse Regional Resource Center (MMRRC) or the Jackson Laboratory's Cre Repository (see Table 1).
The NIH Neuroscience Blueprint program also funded the GENSAT project led by Nathaniel Heintz of The Rockefeller University to generate BAC-Cre recombinase driver mouse lines (Gong et al., 2007). A total of 288 Cre lines have been generated to date2. Following these efforts, many individual laboratories and institutions also produced a variety of Cre drivers (e.g., Madisen et al., 2010, 2015; Leão et al., 2012). Altogether, at least over 630 Cre lines have been deposited at the Jackson Laboratory to date.
The European Union (EU) also followed the NIH initiative and launched its own Cre-driver project under the name CREATE (Coordination of resources for conditional expression of mutated mouse alleles) (Table 1). The CREATE consortium represents a core of major European and international mouse database holders and research groups to develop a strategy for the integration and dissemination of Cre-driver strains for modeling aspects of complex human diseases in the mouse. These Cre lines can be found by contacting EMMA (European Mouse Mutant Archive, Italy) or MSR (International Mouse Strain Resource; Table 1). In addition, the United Kingdom, Canada, and Japan have also funded several Cre-driver projects (Table 1).
Cre-Driving Toward Multiple Directions into the Future
In the past decade, one of the most exciting developments is optogenetics which uses light stimulation to manipulate neurons (Zemelman et al., 2002; Boyden et al., 2005). It has been great fun to witness that optogenetics was able to take full advantage of a decade's investment in generating a rich collection of Cre mouse lines (e.g., CaMKII::Cre, PV::Cre, SOM::Cre, D1: Cre, D2::Cre, ChAT:Cre, or DAT::Cre, etc.), enabling its widespread use in the neuroscience community (Madisen et al., 2012).
However, optogenetics was not the only game in town that benefited from Cre-loxP neurogenetics. Chemical genetics has also emerged as a powerful approach in allowing scientists to activate or suppress specific neurons. For example, one can over-express chemically–genetically modified proteins, such as kinases (Bishop et al., 2000; Wang et al., 2003), or DREADD (Rogan and Roth, 2011) in specific neurons or brain regions. Researchers can also use the floxed diphtheria toxin fragment A (DTA) to delete specific cells using cre lines (Brockschnieder et al., 2004; Matsumura et al., 2004; Buch et al., 2005; Ivanova et al., 2005), and then observe the resulting phenotypes.
In addition, Cre-lox neurogenetics are used to track neural projections in the brain with retrograde virus (Sun et al., 2014; Beier et al., 2015) or CLARITY methods (Lerner et al., 2015). Jeff Lichtman and Joshua Sanes have cleverly explored the unique properties of the Cre-loxP system to randomly express different ratios of red, green, and blue derivatives of green fluorescent protein (GFP) in individual neurons, a technique termed Brainbow (Livet et al., 2007). This allowed them to flag each neuron with a distinctive color. This process has been a major contribution to the field of connectomics.
Cre-lox neurogenetics has also been combined with voltage-sensitive proteins to monitor changes in neuronal activity. Genetically encoded calcium indicators, such as the GCaMPs, have allowed researchers to infer changes in neuron activations via calcium transients (Tian et al., 2009). Researchers are also developing other genetically encoded voltage-sensitive fluorescent proteins that can report cortical electrical responses in vitro and/or in vivo (Akemann et al., 2010; Ghitani et al., 2015; Madisen et al., 2015).
Most recently, Stuart Ibsen and Sreekanth Chalasani reported an exciting way to use low-pressure ultrasound stimulation to produce microbubbles that amplify the mechanical deformations which can be detected by TRP-4, the pore-forming subunit of a mechanotransduction channel (Ibsen et al., 2015). By overexpressing TRP-4 in specific neurons, the authors showed it can sensitize neurons to an ultrasound stimulus. Similarly exciting, Sheng-Jia Zhang and his colleagues recently reported another noninvasive method, namely magnetogenetics, to activate neurons in vivo using magnetic stimulation. The activation of neurons was achieved by neuronal expression of an exogenous magnetoreceptor, an iron–sulfur cluster assembly protein 1 (Isca1) (Long et al., 2015). These two noninvasive approaches offer additional tools to manipulate specific neurons in behaving animals.
Looking back, Cre-lox neurogenetics was written off as a dead-end project, but my intuition and diligence got me through the door. Nowadays, a week rarely goes by without having yet another Cre-based neuroscience finding reported. Cre-lox neurogenetics seems to still have a lot of miles ahead of it. To my students, I always say that being young and foolish can be an asset for the community!
Author Contributions
The author confirms being the sole contributor of this work and approved it for publication.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by the Georgia Research Alliance and National Institutes of Health (R01NS079774). I also would like to express my gratitude for the unique opportunity of working at the Brain Decoding Project Consortium organized by Banna Biomedical Research Institute and supported by Yunnan Science Commission (2014DG002). I am also grateful to the Frontiers journal and editors for their kind invitation to contribute to this special issue.
Agranoff, B. W., and Klinger, P. D. (1964). Puromycin effect on memory fixation in the goldfish. Science 146, 952–953. doi: 10.1126/science.146.3646.952
Akemann, W., Mutoh, H., Perron, K., Rossier, J., and Knöpfel, T. (2010). Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins. Nat. Methods 7, 643–649. doi: 10.1038/nmeth.1479
Beier, K. T., Steinberg, E. E., DeLoach, K. E., Xie, S., Miyamichi, K., Schwarz, L., et al. (2015). Circuit architecture of VTA dopamine neurons revealed by systematic input-output mapping. Cell 162, 622–634. doi: 10.1016/j.cell.2015.07.015
Bishop, A. C., Ubersax, J. A., Petsch, D. T., Matheos, D. P., Gray, N. S., Blethrow, J., et al. (2000). A chemical switch for inhibitor-sensitive alleles of any protein kinase. Nature 407, 395–401. doi: 10.1038/35030148
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Keywords: Cre-loxP, optogenetics, brainbow, mouse brain, learning and memory, neural circuits, cognition, Cre recombinase
Citation: Tsien JZ (2016) Cre-Lox Neurogenetics: 20 Years of Versatile Applications in Brain Research and Counting… Front. Genet. 7:19. doi: 10.3389/fgene.2016.00019
Received: 04 December 2015; Accepted: 01 February 2016;
Published: 19 February 2016.
Edited by:Robert Gerlai, University of Toronto, Canada
Reviewed by:Claudio V. Mello, Oregon Health & Science University, USA
Rafael Fernández-Chacón, The Neuroscience Institute at Seville, Spain
Loren Martin, University of Toronto, Canada
Copyright © 2016 Tsien. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Joe Z. Tsien, firstname.lastname@example.com


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Blog Archives
BBC Launching Free-to-Play Educational Doctor Who Game
The BBC has announced that it will be launching a new Doctor Who game online this week. Titled The Doctor and The Dalek the game will help teach children basic computer coding skills in accordance with the UK's Key Stage 2 and 3 computing curriculum.
It features voicework from The Doctor himself, Peter Capaldi, as well as an original story written by Phil Ford. Jo Pearce of BBC Wales Interactive explained the concept of the game, saying:
Children will be learning these increasingly important new skills while being actively entertained. The idea behind it is simply to use one of our biggest, most popular brands to inspire children to find out more about programming.
The game will be available on the CBBC website from Wednesday, you can watch the trailer below.
Keeley Hawes to Guest Star in Doctor Who Series 8
The BBC has announced that Keeley Hawes (Ashes to Ashes, Line of Duty) is to star in an episode of Doctor Who. Hawes will play Ms. Delphox, who is described as 'a powerful out of this world character with a dark secret.' The episode will see the Doctor (Peter Capaldi) and Clara (Jenna Coleman) 'come face to face with the mysterious Ms Delphox when they arrive on a strange and puzzling planet.'
Steve Thompson, who wrote previous Doctor Who episodes Journey to the Centre of the Tardis and The Curse of the Black Spot, is on writing duty while Douglas Mackinnon, who worked with Hawes on Line of Duty, will direct.
Keeley Hawes said on landing the role:
I am delighted to join Doctor Who and to be working with this incredible team. Ms Delphox is a great character and someone I've had a lot of fun playing.
While Showrunner, Steven Moffat, added:
Anyone watching Jed Mercurio's amazing Line of Duty will know that Keeley Hawes is having one hell of year. And now it's about to get even better as she achieves the greatest villainy yet attempted on Doctor Who: she plays a banker.
The announcement was made at midnight on Doctor Who's official Facebook page, which could hint at an added importance for the character, with such announcements usually reserved for major news such as Matt Smith's departure. With Hawes' character having a 'dark secret' could she be someone from the Doctor's past? We may have to wait until the Autumn, when the new series premieres, to find out.
Doctor Who Gets New Recurring Character for Series 8
Doctor Who is getting a new recurring character played by Samuel Anderson (The History Boys, Gavin & Stacey, Emmerdale) for Peter Capaldi's first series as the Doctor. Anderson will play Danny Pink, a teacher at Coal Hill School, where Clara Oswald (Jenna Coleman) also works.
Anderson said of being cast:
I was so excited to join Doctor Who I wanted to jump and click my heels, but I was scared I might not come down before filming started! It's a quintessential part of British culture and I can't believe I'm part of it. It's an honour to be able to work alongside Peter Capaldi and Jenna Coleman and I can't wait to show people how my character becomes involved with such a fantastic duo!
Showrunner Steven Moffat added:
'For the fourth time in Doctor Who history, Coal Hill School is coming to the aid of the TARDIS. In 1963 teachers Ian Chesterton and Barbara Wright accompanied the First Doctor. These days it's the turn of Jenna Coleman as Clara Oswald. And very soon now, Sam Anderson as Danny Pink will be entering the world of the Doctor. But how and why? Answers are coming later this year in Peter Capaldi's first series of Doctor Who!
Series 8 will begin airing later this year, with filming currently underway on episode 4, guest starring Robert Goodman (Gangs of New York, Game of Thrones) and episode 5 which will co-star Jonathan Bailey (Broadchurch) and Pippa Bennett-Warner (The Smoke, Death in Paradise).
Doctor Who: The Day of The Doctor Official Trailer
The BBC has released the official trailer for The Day of The Doctor, the 50th Anniversary special of Doctor Who. You can watch it below:
A full synopsis of the episode has also been released as follows:
The Doctors embark on their greatest adventure in this 50th Anniversary Special: in 2013, something terrible is awakening in London's National Gallery; in 1562, a murderous plot is afoot in Elizabethan England; and somewhere in space an ancient battle reaches its devastating conclusion.
All of reality is at stake as the Doctor's own dangerous past comes back to haunt him.
The moment is coming…
The episode will broadcast simultaneously around the globe on 23 November, starting at 7.50pm GMT.
'Doctor Who: 50 Years' Trailer Revealed
The BBC has released a special trailer celebrating 50 years of Doctor Who. It features all eleven incarnations of the Doctor as well as many famous companions and enemies from the show's long history. The trailer does not actually contain any footage from The Day of The Doctor but is nevertheless sure to get fans excited for the anniversary episode.
There is just over a month left to wait now until The Day of The Doctor is broadcast simultaneously around the globe on November 23.
New Images from The Day Of The Doctor
The BBC has released six new images from The Day Of The Doctor, the 50th Anniversary special of Doctor Who. The episode is set to be simulcast globally on November 23, a first for the series, and stars Matt Smith, David Tennant, John Hurt, Jenna Coleman and Billie Piper. Check out the rest of the images in the gallery below.
Lost Doctor Who Episodes Discovered
Nine previously missing episodes of Doctor Who from the 1960's have been discovered at a TV station in Nigeria. The episodes all come from Patrick Troughton's second Doctor era, specifically the stories The Web of Fear and The Enemy of the World. The episodes were lost when the BBC destroyed many of the tapes in the 1960's and 70's following their original transmission, fortunately many episodes were copied to film and sold to foreign broadcasters as was the case here.
Restored versions of the episodes are now available to buy on iTunes. You can find trailers for both serials below.
[SOURCE: BBC News]
Doctor Who 50th Anniversary Sneak Peek
The BBC have released the above image from behind the scenes of 'The Day of The Doctor', the 50th anniversary special of Doctor Who, revealing the involvement of the Daleks in the episode. Alongside the image it was announced that two 'stings' for the episode will air on BBC One tonight, before and after the new fantasy series Atlantis, which starts at 8.25pm. These stings will reveal the new ident for the episode as well as a twitter hashtag.
A trailer for the episode is also in the works which the BBC promises will be released 'soon' as the hype machine swings into full gear approaching the episode's air date on 23 November.


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What is an employment contract? What should it cover? What does it need to align with?
Join us for a one-hour webinar that addresses each of these questions and what the basic legal obligations are when writing an employment contract. We'll provide useful tips and hints.
David is Managing Director of the Australian Health Industry Group which advises the Community, Health and Business Services sectors on HR, IR, legal and management issues.
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His past roles include working as the CEO of a small rural health service, Executive and Corporate Counsel of a large metropolitan health service and Legal and Management Consulting throughout Australia.
David's special areas of practice are administrative law, information technology, contract and commercial law, disciplinary tribunals, employment and industrial law, equal opportunity, governance, internal audits, occupational health and safety, workcover, risk management and strategic planning.
David's simple pragmatic approach, combined with real life examples, makes his training sessions informative and thought provoking.Last Updated: 2018-05-24 5:33 PM


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Golf is a sport where you have to practice a lot to learn the golf swing basics and keys. Even if you are an experienced golfer, you need to practice to keep your handicap. There is an unlimited scope for improvements in the golf sport.
Before you go on, you have to decide which golf swing suits you. Every golfer's dream is a 300-yard ball down the fairway. Well, not everybody has the capability to do so. Mental and physical fitness differs from golfer to golfer.
To find the swing that suits your needs you have to understand golf swing basics and adjustments.
Here are some points that you have to keep in mind if you want to improve your game.
The swing plane is a crucial point. You should have the ability to rotate your upper body while maintaining a good swing plane. It is a good idea to record your movements and analyze your swing. This way you will be able to adjust your game.
Another golf swing key is the right speed. A lot of golfers find it difficult to increase their speed. It requires strength and fitness to do properly and is trainable with a decent golf club and guide. You have to improve your core strength to maximize power and distance.
Practice, yes practice, as much as you can. You need to be on top of your body's fitness to play your best golf swing. If you don't train your muscles on a regular basis, they are not able to make the core golf swing moves.
The right grip and stance is another essential point that you have to keep in mind. These 2 points are no mystery, and as you can see, there are no hidden secrets to golf success. Just practice the right way. There are ways to make improvements to your game.
A good training routine will strengthen your swing, and you will take your game to the next level. That is for sure. But how can you exercise efficiently? You need an excellent golf training guide and the right drills. Next, you will want to get the best guides available and better your golf swing basics.
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Essay / Current Affairs
ROADMAP TO HELL: Sex, Drugs and Guns on the Mafia Coast
by Barbie Latza Nadeau
March 2018 – Oneworld
From sex slaves to drug mules, The Daily Beast's Rome Bureau Chief uncovers a terrifying and intricate web of criminal activity right on Europe's doorstep.
Caught between Camorra gunrunners selling to ISIS and Nigerian drug gangs along Italy's picturesque coast, each year thousands of refugees and migrants are lured into their underworld, forced to become sex slaves, drug mules or weapon smugglers. In this powerful exposé, investigative journalist Barbie Latza Nadeau follows the weapons trail, meets the trafficked women trapped by black magic, the brave nuns who try to save them and the Italian police who turn a blind eye as the most urgent issues facing Europe play out in broad daylight.
Barbie Latza Nadeau is an American journalist in Rome who has spent the last 20 years covering crime in Europe, Italian politics, the Vatican, the refugee crisis and women's issues for Newsweek, The Daily Beast, and CNN. Her previous book about the murder trials of Amanda Knox, ANGEL FACE (2010), was adapted for film by Michael Winterbottom (The Face of an Angel, 2015), starring Kate Beckinsale as a journalist based on Barbie herself.
"Barbie Latza Nadeau dissects the intricate relationship between those who make a living from organized crime, terrorism and sex trafficking with an astute understanding of the Italian culture that allows it to prosper. The book takes you through the seedy world of prostitutes and their clients, introduces you to nuns and saviors and exposes one of Italy's darkest secrets." ―Michael Winterbottom, filmmaker, Welcome to Sarajevo, The Road to Guantanamo, A Mighty Heart
"This is a terrifying and heartbreaking book. So many of the world's most alarming issues converge on one street to the south of Naples: enforced prostitution (slavery), human trafficking and the refugee crisis, the Mafia, the drug and arms trade and Jihadi terrorism. Nadeau connects them, showing us a vast, organised economic system and everything required to maintain it: a willingness to exploit desperation, unimaginable brutality and, crucially, the indifference of the "clients," the authorities … and the rest of us. And then she introduces us to the girls themselves – and to the extraordinary women who try to help them, their compassion pervading the entire story. I will find it difficult, from now on, to contemplate today's world without thinking about what goes on in Castel Volturno." ―Colin Firth


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It seems like every year there is a shortage of help in the green industry. I hear horror stories of H2B employees being weeks late to show up for work. Typically, they are held up because of incomplete or incorrect paperwork. read more
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text:
Possible Hot Deals - Amazon Coupon Page
FMB1 last edited by
I have no idea if this is the place to post this, but this page evidently shows ALL discount coupon on Amazon.
Dionaea last edited by
Great find - I didn't know that page was there.
fivetalents Global Moderator last edited by fivetalents
@dionaea said in Possible Hot Deals - Amazon Coupon Page:
Great find - I didn't know that page was there.
See this reference thread for this and many more links/shortcuts to useful pages on Amazon: https://phatwalletforums.com/topic/57/amazon-not-so-secret-links-and-tips-continued/