text:
- Research article
- Open Access
- Open Peer Review
Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors
© Heidenblad et al; licensee BioMed Central Ltd. 2008
- Received: 15 October 2007
- Accepted: 31 January 2008
- Published: 31 January 2008
Urothelial carcinoma (UC) is characterized by nonrandom chromosomal aberrations, varying from one or a few changes in early-stage and low-grade tumors, to highly rearranged karyotypes in muscle-invasive lesions. Recent array-CGH analyses have shed further light on the genomic changes underlying the neoplastic development of UC, and have facilitated the molecular delineation amplified and deleted regions to the level of specific candidate genes. In the present investigation we combine detailed genomic information with expression information to identify putative target genes for genomic amplifications.
We analyzed 38 urothelial carcinomas by whole-genome tiling resolution array-CGH and high density expression profiling to identify putative target genes in common genomic amplifications. When necessary expression profiling was complemented with Q-PCR of individual genes.
Three genomic segments were frequently and exclusively amplified in high grade tumors; 1q23, 6p22 and 8q22, respectively. Detailed mapping of the 1q23 segment showed a heterogeneous amplification pattern and no obvious commonly amplified region. The 6p22 amplicon was defined by a 1.8 Mb core region present in all amplifications, flanked both distally and proximally by segments amplified to a lesser extent. By combining genomic profiles with expression profiles we could show that amplification of E2F3, CDKAL1, SOX4, and MBOAT1 as well as NUP153, AOF1, FAM8A1 and DEK in 6p22 was associated with increased gene expression. Amplification of the 8q22 segment was primarily associated with YWHAZ (14-3-3-zeta) and POLR2K over expression. The possible importance of the YWHA genes in the development of urothelial carcinomas was supported by another recurrent amplicon paralogous to 8q22, in 2p25, where increased copy numbers lead to enhanced expression of YWHAQ (14-3-3-theta). Homozygous deletions were identified at 10 different genomic locations, most frequently affecting CDKN2A/CDKN2B in 9p21 (32%). Notably, the latter occurred mutually exclusive with 6p22 amplifications.
The presented data indicates 6p22 as a composite amplicon with more than one possible target gene. The data also suggests that amplification of 6p22 and homozygous deletions of 9p21 may have complementary roles. Furthermore, the analysis of paralogous regions that showed genomic amplification indicated altered expression of YWHA (14-3-3) genes as important events in the development of UC.
- Bacterial Artificial Chromosome
- Gene Copy Number
- Bacterial Artificial Chromosome Clone
- Urothelial Carcinoma
- Homozygous Deletion
Urothelial carcinoma (UC) is characterized by nonrandom chromosomal aberrations, varying from one or a few changes in early-stage and low-grade tumors, to massively rearranged karyotypes in muscle-invasive lesions [1]. Rearrangements of chromosome 9, resulting in loss of chromosome 9 material, are the most common cytogenetic alterations and are seen in close to half of the cases. Loss of material from chromosome arm 11p, and gains of chromosome 7 and chromosome arm 1q seem to be early but secondary changes. UC is also characterized by a strong association between tumor stage/grade and karyotypic complexity, indicating that progressive accumulation of genetic alterations is the driving force behind multi-step bladder carcinogenesis [2]. Cytogenetic data have been corroborated by comparative genomic hybridization (CGH) investigations [3–5], which in addition have been valuable for identification of high-level amplifications. Recent array CGH analyses of bladder tumors [6, 7] and cell lines [8], based on non-tiling bacterial artificial chromosome (BAC) arrays have shed further light on the genomic changes underlying neoplastic development. These studies have facilitated the molecular delineation of additional amplified and deleted regions to the level of specific candidate genes. Recurrent high-level local amplifications have so far been reported in many genomic regions, including 1q21-24, 3p22-24, 6p22, 8q21-22, 10p13-14, 11q13, 12q13-15, and 17q21 [3–8]. To provide a more complete and detailed map of previously observed amplified and deleted segments, and to identify new copy number aberrations (CNAs) of importance to urothelial tumorigenesis, we used whole-genome tiling-resolution array CGH based on a 32 k BAC clone set to analyze 38 primary urothelial carcinomas. In order to allow a comprehensive analysis also of transcriptional effects of the identified CNAs, the copy number analysis was complemented with expression profiling using high-density (36 k) oligonucleotide micro arrays. With this approach we present genomic borders and putative target genes for 9 recurrent amplifications, some of which specific for advanced tumors, and for 7 previously not described homozygous deletions, of which one is recurrent. We also studied the pattern of CNA occurrence, and show that 6p22 amplifications are not independent of homozygous deletions in 9p21, and that amplification of paralogous segments in 8q22 and 2p25 appear to act complementary and may have equivalent biological outcomes as they both result in increased expression of members of the YWHA genes, encoding 14-3-3 proteins.
Patients and tissue
Tumors were collected by cold-cup biopsies from the exophytic part of the bladder in patients undergoing resection at the University Hospital of Lund, Sweden, between 2001 and 2004 and kept at -80C until further use. Sample quality was evaluated by histology. Altogether 10 Ta, 9 T1, and 19 muscle-invasive (T2-T4) tumors were included in the study. Of these 15 were low grade (grades G1 and G2) and 23 high grade (G3) tumors. Tumor pathology, based on transurethral and cystectomy specimens, was assessed by an experienced pathologist (GC) and is listed together with other clinical data in Additional file 1. The investigation was approved by the local ethical committee at the Lund University and a written consent was obtained from all patients.
Nucleic acid isolation
DNA was isolated using the DNeasy Tissue Kit (Qiagen, Valencia, CA), including the optional RNase H treatment, and verified for high quality by agarose gel electrophoresis. Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA). Isolated RNA was purified on Qiagen RNeasy columns (Qiagen) and sample integrities were assessed on an Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA).
Microarray hybridizations
Labeling of test and reference DNA was performed as previously described [9], with slight modifications. In brief, 1.5 μg of tumor and male reference DNA was fluorescently labeled with Cy3-dCTP and Cy5-dCTP (Amersham Biosciences, Uppsala, Sweden), respectively, using the Array CGH labeling kit (Invitrogen, Carlsbad, CA), and purified using filter-based spin columns (Cyscribe GFX Purification kit, Amersham Biosciences). Differentially labeled DNA was pooled, mixed with 100 μg Human Cot-1 DNA (Invitrogen), and lyophilized prior to resuspension in 55 μl hybridization solution (50% formamide, 10% dextran sulfate, 2× SSC, 2% SDS, 10 μg/μl yeast tRNA). Probes were heated at 70°C for 15 minutes and at 37°C for 30 minutes before hybridization to micro arrays for 48-72 hours at 37°C. High-resolution tiling BAC arrays produced at the Swegene DNA Micro Array Resource Center, Department of Oncology, Lund University, Sweden [10] using the BAC Re-Array set Ver. 1.0 (32,433 BAC clones) previously described [11] was used. The BAC Re-Array set was obtained from the BACPAC Resource Center at Children's Hospital Oakland Research Institute, Oakland (CA). Prior to hybridization, micro arrays were UV-cross linked at 500 mJ/cm2 and pretreated using the Universal Micro array Hybridization Kit (Corning, Acton, MA) according to the manufacturer's instructions. Slides were washed and scanned as previously described [12]. Oligonucleotide arrays printed with 70-mers from the OPERON v 3.0 set were obtained from the Swegene DNA micro array resource centre [10]. The 36,288 oligonucleotides printed on each slide correspond to 18,466 unique Entrez genes. Sample and Universal Human Reference RNA (Stratagene, La Jolla, CA) labeling, and micro array hybridization was performed using the Pronto Plus System (Promega, Madison, WI; Coring, Acton, MA) according to the manufacturer's specification. Arrays were scanned with an Agilent G2565AA micro array scanner (Agilent technologies).
Microarray image and data processing
Primary data were collected using the GenePix Pro 4.0 software (Axon Instruments Inc., Foster City, CA), and raw result files were deposited into the web-based database BioArray Software Environment (BASE) [13]. For genomic profiling, spots were background-corrected using the median foreground minus the median background signal intensities for both dyes, and log2 ratios were calculated. Unreliable features, marked in the feature extraction software, and spots not showing signal-to-noise ratios ≥ 3, for both channels, were removed. Data normalization was performed per array subgrid using Lowess curve fitting [14] with a smoothing factor of 0.33. Chromosomes X and Y BAC clones were omitted during the estimation of the normalization function. The BASE implementation of CGH-Plotter [15] was used to produce imbalance frequency plots in which BACs were defined as gained, normal, or lost using gain/loss log2 ratio thresholds of ± 0.25, and a moving mean sliding window of five clones. For amplification frequency plots, amplifications were defined as regions with at least two consecutive BAC clones showing log2 ratios ≥ 1.0. Amplicon sizes were defined as the longest distance between the two outermost amplified BAC clones. In cases of highly discontinuous amplification, amplicons were considered ended when separated by at least two BAC clones with log2 ratios below 0.5. Homozygous deletions were defined as regions with consistent log2 ratios below -1.2, and with at least one BAC showing a log2 ratio < -1.5. Mapping data were obtained from the UCSC genome browser [16]. To identify genomic amplifications specific for G3 tumors a significance analysis of micro arrays (SAM) was performed [17]. Selection of amplification target genes was based on three criteria. Potential target genes should ideally display i) top ranking expression levels in cases with highest DNA copy number of that particular gene, ii) a high Spearman rank correlation throughout the entire data set, and iii) at least 2-fold up regulation when amplified compared to median expression of that gene in the data set.
Real-time quantitative RT-PCR analysis and genomic PCR analysis
Custom-made TaqMan probes for the test genes and for three internal standards (ACTB, HPRT1, and RPLP0) were obtained from Applied Biosystems (Foster City, CA.), and reactions performed on a Real Time PCR System 7500 (Applied Biosystems) according to the manufacturer's recommendations. To verify potential homozygous deletions sequence-tagged site (STS) markers within the deleted regions were used for semi-quantitative genomic PCR analysis. Quantification of PCR products were performed as previously described [18].
Genomic imbalances categorized according to grade and stagea, b
Summary of common recurrently amplified regionsa, b
Cytogenetic region
Mb start position (BAC)
Mb end position (BAC)
Size (Mb)
No. cases
3 (8%)
4 (11%)
3 (8%)
3 (8%)
8 (21%)
5 (13%)
3 (8%)
3 (8%)
3 (8%)
The 6p22 amplicon
NUP153 and FAM8A1 showed a strong association between gene expression and gene copy numbers (Additional file 3). No BAC clones corresponding to the genes TPMT, AOF1 and DEK were available after the final filtration of the BAC array data, however, both AOF1 and DEK showed substantial correlations between gene expression and gene copy numbers as estimated by BAC clones flanking the genes. The tumors that showed the highest gene copy numbers for NUP153, AOF1 and DEK were also the top ranking cases with regard to expression. In the core amplicon six and four of the seven cases with SOX4 and MBOAT1 amplifications, respectively, were top ranking with respect to expression. Because no microarray expression data were available for three of the most commonly amplified genes, E2F3, CDKAL1 and ID4, these were analyzed separately by real-time quantitative PCR. This analysis showed a moderate copy number dependent expression for ID4 but a strong association between the DNA and mRNA levels for CDKAL1 and E2F3 (Additional file 4). Hence, a strong link between gene copy numbers and gene expression is seen for the frequently amplified genes MBOAT1, E2F3, CDKAL1, and SOX4 as well as for the less frequently amplified NUP153, AOF1, FAM8A1, and DEK.
The 8q22 and the related 2p25 amplicon
Homozygous deletions
We performed whole-genome tiling-resolution array CGH analyses to characterize gene copy number aberrations in 38 cases of urothelial carcinomas. To make a comprehensive evaluation of the consequences at the expression level the array CGH data was compared with expression data obtained by high density expression profiling. Low grade and early stage tumors showed a limited number of genomic imbalances with gain of 1q and loss of chromosome 9q as the most frequent changes. T1 tumors harbored additional changes and frequently showed losses of 6q, 8p, 9q, 11p, 17p, 19p, and 22q, and gains of 1q, 5p, 8q, 10p, and 13, and 5p. Late-stage tumors, T2-T4, showed several additional genomic imbalances and showed complex genomic changes. A major difference was seen between G1/G2 and G3 tumors with regard to gains and amplifications. Gains were more frequent in G3 tumors and amplifications more than 10 times as frequent in high grade than in low/medium grade tumors. This is in line with the recent finding that high grade tumors show frequent anaphase bridges that ultimately may lead to breakage-fusion-bridge cycles producing genomic amplifications [19]. In contrast to Blaveri et al. [7], we did not see a reduction in the number of affected regions in late-stage tumors. Instead T2-T4 tumors showed considerably more complex changes than Ta/T1 tumors, corresponding to previous cytogenetic and conventional CGH results that have shown a larger number of genomic alterations in high-grade and late-stage tumors [2–4, 20].
The analysis showed that amplifications of 1q23 were highly heterogeneous. Individual cases showed extended amplicons with peak copy number ratios in different areas and with no obvious commonly amplified segment. Gain of chromosome arm 1q is frequent in UC [1, 2] and local amplifications in 1q23 have previously been reported in studies using conventional CGH [3, 5]. However, due to limited resolution in traditional CGH analysis diverse amplifications within a 5 Mb genomic region may have been interpreted as a common genomic event and hence assumed to target the same genomic segment. Interestingly, the present tiling resolution analysis indicates that this is most likely not the case. There are at least two possible explanations for these findings; either the 1q23 region harbors more than one target segment, or the 1q23 region show local genomic instability resulting in frequent gains or high level amplifications. Both situations would result in a heterogeneous pattern of changes.
The most frequently amplified chromosomal segment was 6p22. This segment has been repeatedly shown to be amplified in UC and to cover the genes E2F3, CDKAL1, and SOX4 [6, 8, 21–25]. In the present investigation a 1.8 Mb core region, included in 88-100% of the cases with 6p22 amplifications, was defined. This region contains MBOAT1, E2F3, CDKAL1, and SOX4. The core segment was flanked by two regions included in 38-75% of the amplifications that on the distal side contained ID4, and on the proximal side PRL and HDGFL1. A further distally located region, present in three (38%) of the cases with amplification, contained ten additional genes. In total a more than 6 Mb region was found to be repeatedly included in the 6p22 amplifications. To identify possible targets genes within this segment we used different criteria based on available expression data. Six genes; NUP153, AOF1, DEK, SOX4, FAM8A1, and MBOAT1 showed top ranking expression levels when amplified. By Q-PCR we also established a strong association between E2F3 and CDKAL1 gene expression and gene copy numbers. This identifies eight possible candidate genes in 6p22 that may be ranked according the frequency by which they take part in 6p22 amplifications as CDKAL1, E2F3, and SOX4 > MBOAT1 > DEK, NUP153, FAM8A1, and AOF1.
Several investigations have shown a strong association between gene copy numbers and gene expression for E2F3 and CDKAL1 [8, 21, 22]. In two recent studies knock down experiments of E2F3 in bladder cancer cells lines with 6p amplification was shown to strongly reduce cellular proliferation whereas a similar knock down of CDKAL1 did not have this effect [26, 27]. This indicates E2F3 as a major target for the 6p22 amplification. The E2F3 locus produces two alternative proteins, E2F3a and E2F3b [28]. E2F3a is an inducible activator of cell cycle progression whereas E2F3b is constitutively expressed and functions as a negative regulator of p14 ARF expression, thereby compromising TP53 activity [29]. Genomic amplification of the E2F3 locus may consequently contribute to tumor development both by accelerating the cell cycle and by raising the threshold for apoptosis via inactivation of p14 ARF . In this context it is worth noting that none of the cases with 6p amplification showed homozygous deletions leading to loss of p14 ARF . Hence, amplification of 6p and homozygous deletion of the CDKN2A/CDKN2B locus behaves as complementary events; one leading to the simultaneous loss of CDKN2A, a cell cycle inhibitor, and p14ARF activity, and a second to increased E2F3, a cell cycle activator, and reduced p14 ARF activity. As a consequence 65% of the G3 tumors may show impaired p14 ARF function.
SOX4 belongs to the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in cell fate determination. The protein acts as a transcriptional regulator in the apoptosis pathway as well as in pathways leading to tumorigenesis. SOX4 is over expressed in several tumor types [30] and was found to be significantly over expressed in bladder tumors in a recent investigation [31], suggesting a role for SOX4 in bladder cancer development. Intriguingly, both experimentally increased [31] and decreased [32]SOX4 expression induces an apoptotic response. This may indicate that SOX4 expression has to be finely tuned not to induce deleterious cellular responses and may, partly, explain why no consistent correlation between SOX4 amplification and expression has been seen in previous investigations [8, 21, 31].
The MBOAT1, DEK, FAM8A, AOF1, and NUP153 genes were amplified at lower frequencies than E2F3. Of these DEK, a chromatin-associated protein could provide a selective advantage when amplified as DEK function as an inhibitor of senescence [33]. Furthermore, as DEK is under the transcriptional control of E2F3 [34] co-amplification of DEK with E2F3 may enhance DEK expression further. NUP153 plays an important role in nuclear pore function and have key functions in both import and export to the nucleus. NUP153 is known to affect the sub cellular distribution of transcription factor such as SMAD2, STAT1, and PU.1 [35], hence, altered NUP153 expression may modify the accessibility of key regulatory proteins. The possible roles of MBOAT1, FAM8A1, and AOF1 in carcinogenesis are, however, less clear. Taken together, the present comprehensive analysis of 6p22 amplification in UC indicates that SOX4, DEK and NUP153 may contribute to cellular transformation when co-amplified with E2F3, and hence that 6p22 may represent a composite amplicon with more than one potential target gene.
The core amplicon in 8q22 extended over a 1.8 Mb region and included 8 annotated genes of which YWHAZ (14-3-3-zeta) and POLR2K showed a strong association between gene copy number and gene expression. Interestingly, three tumors with no amplification of 8q22 showed amplification of a 1 Mb paralogous region in 2p25. This segment included several genes similar to genes within the 8q22 segment, such as YWHAQ, GRLH1,HCAL1, RRM2, and KLF11. Two of the paralogous genes within the 2p25 amplicon showed a strong association between gene copy numbers and gene expression, YWHAQ (14-3-3-theta) and RRM2. Hence, the common denominator of the 8q22 and 2p25 amplicons is the amplification and over expression of the paralogous 14-3-3-zeta and 14-3-3-theta genes. Furthermore, if amplification of the YWHA genes may substitute for each other, amplification of these genes is as common as amplification of E2F3.
There are seven 14-3-3 isoforms described in mammals [36]. The proteins are widely expressed and bind as dimers to client proteins thereby modulating their enzymatic activity, sub cellular localization, or potential to form protein complexes; more than 200 proteins have been reported to associate with these proteins. It is believed that 14-3-3 proteins functions as general survival factors by enhancing pro-survival signaling and suppressing pro-apoptotic proteins [36]. The findings that 14-3-3-zeta and -theta show increased expression in oral squamous cell carcinomas and lung, stomach, and breast cancers [37–40], and that mice transgenic for 14-3-3-zeta develop various types of tumours at young age [41] favors the conclusion that 14-3-3-zeta and -theta may act as oncogenes also in urothelial carcinomas.
Homozygous deletions were detected in six locations on chromosome 9 and in four locations in the remaining genome; 4q35, 10q23, 13q14, and 21q21. None of the observed deletions coincides with deletions frequently seen in cell lines [42] and only one, 9p21, has previously been reported for urothelial carcinomas. Among the deletions not occurring on chromosome 9 three, 4q35, 10q23, and 21q21 respectively, extended over genes not previously associated with tumor development and may merit further analyses. The 13q14 deletion, on the other hand, affected the known tumor suppressor gene RB1, and hence most likely has a pathological effect. As expected the most frequent homozygous deletions occurred at 9p21, in a 4 Mb segment that consistently included CDKN2A and CDKN2B. The observed frequency of CDKN2A homozygous deletions reached 32%, which is close to the frequency found by Q-PCR of micro dissected tumor biopsies [43]. The deletions were more extensive on the proximal than on the distal side of CDKN2A which may be caused by the fact that the proximal region is less dense with genes. An intriguing finding was the clustering of homozygous deletions to chromosome 9. Homozygous deletions were seen in five chromosome 9 locations, excluding the high-frequency CDKN2A region, compared with a total of four homozygous deletions on other chromosomes. Furthermore, one case showed two homozygous deletions, and another case one homozygous deletion in addition to a 9p21 deletion. This high frequency of homozygous deletions is astonishing but is in agreement with the high frequency of LOH and chromosomal 9 losses seen in UC [44]. The accumulated data reveals no specific pattern of LOH, which has led to the suggestion that most of the LOH seen in chromosome 9 may be caused by unspecific mitotic recombination [45]. A possible mechanism for the frequent homozygous deletions could consequently be unequal mitotic recombination events. Such events would produce homozygous deletions after segregation of the recombined homologues, as well as segmental duplications. Indeed, possible segmental duplications on chromosome 9 were seen in a limited number of cases. Irrespective of the mechanisms causing the deletions, the high frequency of homozygous deletions at various locations on chromosome 9 point to the possibility that these deletions may have a different origin and cellular consequence than the more frequent 9p21 homozygous deletions.
The presented data indicates 6p22 as a composite amplicon with more than one possible target gene and suggests that amplification of 6p22 and homozygous deletions of 9p21 may have complementary roles. The combined data for amplified 8q22 and 2p25 paralogous genomic segments indicated that alterations of YWHA (14-3-3) genes may be important in the development of urothelial carcinomas. Furthermore, chromosome 9 show an exceptionally high frequency of homozygous deletions compared to other chromosomes.
This study was supported by grants from the Swedish Cancer Society, the Swedish Research Council, the Gunnar, Arvid and Elisabeth Nilsson Foundation, the Crafoord Foundation, the John and Augusta Persson Foundation, the Inga Britt and Arne Lundberg Foundation, the Maud and Birger Gustavsson Foundation, the Petrus and Augusta Hedlund Foundation. The microarray facility was supported by the Knut and Alice Wallenberg Foundation via the Swegene Program.
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- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1755-8794/1/3/prepub
Pre-publication history
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Jewelry for Your Wedding Day
Look Like You Are Wearing the Real Thing!
Celebrities have the unique advantage of being able to afford the customized wedding of their dreams. Couture gowns and bridal jewelry, whimsical details, and a fabulous cast of famous guests – no wonder we're envious of these super-sized weddings! Emitations makes wedding shopping easier, with celebrity style wedding jewelry and gorgeous red carpet accessories.
Whether you want to emulate the ceremony of a hot Hollywood couple, or just feel like a starlet on your big day, Emitations has all of the bridal fashion jewelry you need! Check out our sterling silver engagement and wedding rings, crystal and rhinestone hair accessories, and glamorous CZ couture necklaces, bracelets, and earrings.
*This post contains affiliate links and we will be compensated if you make a purchase after clicking on links.

subset: commoncrawl


text: There are no words to thank Lewis Marquees and their team for one incredible set up for our wedding. No request was too small, always there to answer never ending questions and the final product was more than we could have dreamed off. The most beautiful Marquees which completed our wedding. I honestly can't recommend them enough or thank them enough.— Flora and Richard, Horsham October 2017


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3 comments:
- Allie said...
I wanted to win, but I also wanted to make it myself, so now I'm motivated! Congrats to the winners!
- October 18, 2010 at 2:15 PM
aaawwwww, the first winning post is only four posts below me - so close *sigh*
but, on the other hand i made one today. i think i somehow ruined my sewing machine with the thick fabric and thread though - nothing serious i think, just need to take it to the shop to readjust.
i swear it was the most complicated thing i ever made and it took me the whole day until now (midnight) to finish but i think it came out great.
no chance to test it just now though, as my little one is sound asleep, but tomorrow we will go for a little walk :-)
thank you SO much for the awesome detailed tutorial, even for a beginner like me it was understandable and possible to follow each step.
congratulations to the winners!
- October 18, 2010 at 3:25 PM
- Cami said...
AHHHHHHHHHH!!!!!! I can't believe I won!! I never have won anything in my entire life! My husband kicked me because I got a little too excited... :)
Oh, and it's perfect because today just so happens to be my 24th Birthday!!
You are the best!!
My email is email@example.com :)
- October 19, 2010 at 1:44 PM


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This post NOT brought to you by Apple/
So, I got this new computer and it has completely sucked all the life out of me and my poor little repetitively strained forearms. Seriously, sucked the life out of me. Like a Dementor sucking the life out of Dudley Dursley. Sucking. Suck-King.
To be fair, it's not so much the new computer as the voice dictation software that was supposed to make my life easier and my forearms all smiley and my limp little carpally tunnelled wrists spring to life. MacSpeech dictate officially sucks in my books right now. It doesn't work, which means that I can't make the computer work, which means that I am dictating this on a wheezing old PC that's starting to look kind of good right now. At least when it doesn't crash on me, like it did ... just ... now. Reboot. Somewhere in Georgia, two tech people are trying to solve this problem for me. I spent 40 minutes on the phone this morning with some drawling guy named Jason and it felt just a little bit like suicide prevention call. "Jason," I kept saying as the call drew to a close, "Jason, I'm scared to get off the phone with you."
"Yeah," he would answer, "I'm kind of leery about that myself."
Despite his compassion, Jason could not make MacSpeech work for me, though.
And yet, I haven't lost all hope. You get hopeful. You do, when the shiny new technology toy arrives in the box and you imagine just how great things might be. You do, when your four-year-old climbs on the school bus on the first day of school last week and you think, "Huh. That was way easier than last year."
And then, and then, the computer sits on your desk like a big shiny expensive mistake, and the second day of school rolls around and your son pulls his knapsack straps over his shoulders and then turns around for one last hug -- hugging! Damn the hugging! -- and completely melts down. Runs back inside the house. Repeats things like "I'm going to miss you too much! I want you all to come with me! I'm going to miss Isaac! I just want to be here with you!" Is driven, sobbing, by his other mother to class, clutching a blanket, continuing to sob. Setting off other kids in the class who weren't quite sure whether this was a good idea or not.
Rachel and I have been kind of mopey all day.
To take the edge off, I cycled to the bus stop after school where Rowan would meet his babysitter. He climbed down, a bit tired but seemingly no worse for wear. Told me he had homework, that we had not put a granola bar in his lunch (peanut allergies in the classroom) and that it was Katie's birthday on Thursday -- and they would celebrate.
"When it's your birthday, should we bring cupcakes to school?" I asked him.
"Yes," he said.
But I'm not convinced. Not convinced that the promise of cupcakes will get him on the bus easily on Thursday. Not convinced that my Mac will be running by then, either. I give them both three weeks to settle into the new school year. It's a tempered kind of hopeful, and yet, it's still there. Because I'm nothing if not an optimist. Or just terribly, terribly naïve.


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This star rating is the opinion of the property owner only. A Self-Rated hotel has not been assessed by the AA or Visit Britain, whose official star ratings we use across LateRooms.com.
Guesthouse at Shepshed Ltd3 Self rated
19-21 Hall Croft, Shepshed, Shepshed, LE12 9AN
Price FromView Rooms
- Very Good
- 17 guest reviews
"It's probably my own fault for not looking closely enough at the online info when I booked BUT, the toilet/shower room turned out to be a sh..."
"Very clean and quite...."
"Clean and quiet...."
"I arrived late and rose early for breakfasts and nothing was too much trouble. Very clean and well thought out comfortable b n b, bigger tha..."
"Good value for money comfortable overnight stay...."
About the hotel
The Guesthouse is situated in the centre of Shepshed with easy access to the M1 J23 only 2 miles away, A42 j13 is only 8 miles away. Prestwold Hall is only 8 miles, East Midlands Airport, Castle Donnington and M1 j24 are 7 miles from us. Leicester, Nottingham and Derby are all within 15 miles of us and easily accessible.
Bookings can be made through our website or by phone if you prefer to speak to us in person. Please note that our normal check in times are between 4 and 9pm
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Facilities Show/hide
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- Tea/Coffee making facilities in each room, Hairdryer in each room
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- Between 16:00 and 21:00
- By 10:30 hours
- Up to 3 years old
- 01/02/2015 to 01/07/2020 - Customers are advised to read our booking and cancellation policies. We also require a check-in time and contact telephone number. The price shown is based on the occupancy and dates you have searched for. Prices and availability are subject to change based on the arrival date, number of nights and occupancy searched for. All prices are inclusive of all local taxes.
- Pets are not allowed
Customer Reviews
Our reviews are from genuine LateRooms.com customers. We verify that the customer has stayed before leaving a review.
Very Good
94% would recommend it to a friend
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It's probably my own fault for not looking closely enough at the online info when I booked BUT, the toilet/shower room turned out to be a sh...
Guesthouse at Shepshed
Very clean and quite....


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Legal Services Redefined
We have created a new law firm model that provides our clients with hands-on, big-firm corporate legal services at fixed fees that make business sense.
- Since the firm's founding in the beginning of 2000, we have been singularly focused on providing corporate legal services to entrepreneurial growth companies (whether start-up, emerging-growth or middle-market) and the investors that finance them.
- We provide blue chip legal services. Our founder, Michael Horten is a US and European educated lawyer with over 40 years' experience as a practicing attorney. Michael began his legal career with Sullivan & Cromwell in New York and Paris. He then spent 25 years with King & Spalding in Atlanta.
- We believe law firms don't need to be in the marble and mahogany clad environment typically associated with a superior legal firm. We understand that clients are tired of paying for needless overhead and overstaffed transactions. We wind-tested the traditional corporate law firm model and removed everything that created drag. As a result, our fees are considerably lower than those charged by firms of comparable ability and quality.
- We believe our clients need predictable fees and our services are provided on a fixed-fee basis. The time-based billing metric discourages efficiencies and it encourages firms to overstaff matters. Clients deserve flat-out expertise, skill and service without worrying that we're just clocking minutes. Our clients purchase our skill; not our time.


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The St. Louis Rams got a head start on Tuesday's NFL trading deadline by acquiring wide receiver Brandon Lloyd from the Denver Broncos.
In return, published reports indicate the Rams will ship a conditional 6th round pick to Denver that becomes a 5th round pick provided Lloyd catches 30 passes over the Rams final 11 games.
With Denver making the decision a week ago to insert second year player Tim Tebow into the starting line up at quarterback, the Broncos have clearly moved into rebuilding mode. The decision to trade Lloyd comes as little surprise given that he is in the final year of his contract and the Broncos were not willing to sign him to an extension with young players such as 2010 1st round pick Demaryius Thomas, 2010 3rd round pick Eric Decker and 2008 2nd round pick Eddie Royal on the roster.
In St. Louis, Lloyd will be reunited with offensive coordinator Josh McDaniels, who coached the Broncos during the 2010 season when Lloyd enjoyed a career year after languishing on several rosters during the first seven years of his career. With McDaniels leading a pass first offense, Lloyd caught 77 passes for 1,448 yards and 11 touchdowns.
With the offense becoming more conservative under new head coach John Fox, Lloyd had seen his opportunities, and production, diminish. During four games this year, he had accumulated just 19 receptions for 283 yards and no touchdowns.
Fantasy Impact
Almost assuredly, Lloyd immediately becomes Sam Bradford's top target in St. Louis. It will also be no surprise if free agent signee Mike Sims-Walker is given his release. Sims-Walker has been a bust for the Rams, dropping several passes which led to him being a game day inactive during this week's game against the Packers.
The question isn't so much whether Lloyd can duplicate his 2010 success with St. Louis (he can't) but whether he can provide the Rams and Bradford with the true number one wide receiver they have lacked for years. On that count, Lloyd should be successful although he doesn't rank amongst the top wide receivers in the league.
Consider solid a low end WR2 or high end WR3 for the balance of the season. If that seems too low, consider the current state of the Rams offense, which has scored two touchdowns in the last three games and put up just three points this week against the Packers.
With Lloyd in town, Danario Alexander's six-reception, 91-yard performance this week got a whole lot less exciting. Nonetheless, it's hard to imagine the Rams scaling him back too a minimal role given his enormous upside. He rates as a low end WR4 for 2011 but remains a solid dynasty prospect provided his gimpy knees holed up.
The biggest losers from the Lloyd acquisition are Sims-Walker and Brandon Gibson. While Sims-Walker was a long shot to see action on game day barring injury, Gibson may become an afterthought with Lloyd in town and the emergence of Alexander and rookie fourth round pick Greg Salas (eight for 77 this week).
In Denver, another fantasy loser is Tebow. The inexperienced signal caller's job gets a whole lot tougher with his best wide receiver shipped out of town. While Eric Decker has looked solid thus far in 2011, he benefitted from the additional attention that Lloyd drew playing opposite him.
Thomas is expected to take Lloyd's place in the starting line up but he has had trouble staying on the field, much less being productive. He missed six games last season due to injury as well as the first five games of this season. Expected to be placed on the physically unable to perform list due to a ruptured Achilles tendon suffered in 2010, Thomas proved healthy enough to play in the preseason but broke a finger on his first day of practice.
Decker becomes the Broncos top threat at wide receiver but remains a low end WR3. Thomas remains a solid prospect in dynasty leagues but there's no point in grabbing him off the waiver wire in redraft formats unless you are truly desperate.
As for Tebow, let's hope you weren't relying on him in the first place.


text:
About Us
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Cold Curve Refrigeration currently services many of the major South African supermarket chains mostly located in or near Gauteng. Plant and equipment is designed, built and installed by employing the latest available technology and practices and thereafter maintained and serviced on a 24/7/365 basis. The company is an industry leader in energy efficient retail and commercial refrigeration systems, including cold storage refrigerated rooms, with Multiplex Systems being their specialty.
Cold Curve Refrigeration is active in set-up and maintenance of refrigeration applications in several national brand convenience stores, food processing, logistics, pharmaceutical and agricultural businesses.
Cold Curve Refrigeration provides maintenance and emergency services on a 24/7/365 basis to all its contract customers. Maintenance plans are designed and implemented with careful consideration to the clients' requirements as far as plant requirements (relating to its age), budget and condition of the plant and equipment are concerned.
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One of the most popular searches on my blog I think is anything HAIR related. No surprise really I guess. Some of the searches that find my blog are about colouring – a good 70% I'd say. With that in mind I am dedicating this Hair Envy to Colour and want to say one of my favourite motos when it comes to hair colour, 'Don't be afraid of Colour'.
For decades people have been colouring their hair, yes there's some really scary ways to do it from back in the 1920's when it became very popular to colour your hair. But, in the last 90+ years of hair dyes they have REALLY come a long way. Now I'm not saying you should run other there and do something drastic – far from it. What I am saying with this post, is there are many ways you can add some colour to your hair – some drastic, some subtle and it really depends on the person as to how far they feel comfortable going. What I am saying is you don't need to do something drastic and scary to give yourself a change.
Blonde – Probably my favourite blonde, Blake Lively. This girl is attractive, alluring and but somehow seems to have that effortless style. Blonde hair ranges in some many shades, I think people all too often think of blonde and think of platinum or very light blonde hair. But surprisingly on a colour chart shade 7 is a dark blonde and still classed as a blonde.
If you are a natural blonde, embrace it! My mum used to go nuts at me for colouring my blonde hair. 'People pay good money to get their hair your colour, ' she would say and she's right they did. But we are never happy with what we have. So, here's some tips for mixing up blonde hair a little.
- If you are a dark blonde, why not add some highlights and get the 'beach' sun kissed look? It doesn't have to be heavy, subtle looks great and it will brighten your complex too.
- If you are a light blonde, do the reverse! Get some low lights in there, subtle ones I'm not talking black or dark brown. Toning down bleach or light blonde hair can make it look less harsh and give you definition and movement.
- Sometimes you just want to add some 'shine' and a hint of colour, so why not use a semi permanent dye? Changing the 'tone' of your hair and not the actual depth colour can be a great way for mixing up a look. It doesn't have to be permanent!
I know its all too easy to reach for the bleach, I've been there and done it! But let's be honest we don't want to make the same mistake as Miss Lohan and look washed out and twice our age now do we?
1 word – euugh?!
I'll be honest and this is a taste thing, I don't really like ultra light blonde hair. Even natural, it can look fake. I like the medium golden blondes. That's my favourite colour.
Many blondes get that brassy look, I know I do when I'm blonde but this is namely as I have warm strawberry blonde undertones in my hair natural (Along with gold and ash – yes my naturally blonde hair never did look natural!?). One good way to help these nasty tones you may hate is by using a purple toning shampoo. One of the ones I have in my bathroom permanently (I use this even on my brown hair when it gets a bit brassy to remove the tones) is John Frieda's Sheer Blonde Colour Renew Shampoo (They also do a matching conditioner which is pretty good too on those blonde locks!). I generally use this every wash till I have the brassiness under control and then probably every other wash (Or at least once a week depending on how frequently you wash your hair).
So, you are blonde and bored what can you do? Well, why not add a semi permanent dye to your hair – something subtle to give it some shine?
Casting Creme Gloss Glossy Blondes – These semi permanent colours are really quite good. They have a nice range of different tones. They won't lighten your natural hair but they will tone it (And can darken it depending on the shade of your hair colour at present). Very good for adding shine and glow to hair without the damaging effects of a permanent hair colour. They are designed to wash out and fade gradually without regrowth.
Word to the wise though – IF you have more than 10% grey hair I WOULD NOT use these! They don't cover grey very well at all.
If you want to change the tone and not the depth, always pick a shade that lighter than your actual hair colour. Also remember, if you have tint on your hair or previous colour, adding additional colour over the top will make it go darker – so as well as your 48 hour sensitivity test make sure you do a strand test too so you know what development time you will need!
Please remember, you are beautiful as you are and your hair is only minor reflection of you! Be safe when colouring too, just google hair colour reactions and you'll see waiting 48 hours and doing a sensitivity test could really be life saving!
Well, that wraps up my don't be afraid of colour – blondes piece. I'll be adding brunettes next – maybe it'll mean I colour my hair again!
Lots of Love,
Mel xxxx


text: Hi to everyone. Welcome to our first blog. Here at Direct Computer Repairs we specialize in computer and laptop repairs. We pride our selves on giving an excellent customer service. We always call our customers to let them know exactly what time we will be arriving and another call to say we nearly there. We are experts in removing viruses without having to format your computer or laptop. Our engineers carry all the parts in their vans and if you require any parts they will have it (hard disks, external hard disk, speakers and all computer hardware and more). We cover most of Essex, Kent, South London, Southeast London, City, West London and North London. We have a map on our website showing the areas covered at http://www.DirectComputerRepairs.com


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Police are investigating a shooting on Long Island that left one man dead and another injured.
According to authorities, police received a report early Sunday morning that two masked men had broken into a home on East Moriches Boulevard in Eastport, announced a robbery and shot two men before fleeing.
Police said 45-year-old Francisco Pirir was pronounced dead and another man who was shot in the hand was treated and released from a local hospital.
Investigators suspect the gunmen targeted the house believing that the seven day laborers living there kept cash inside.
Pirir, a landscaper who was living in the home with his two brothers, was supporting a wife and four kids in Guatemala. Immigrant advocate Margaret Smyth said he was "not doing anything more than trying to live a good life here."
Smyth said day laborers in the area are often crime targets.
"It seems that they won't report what happens, and we've been encouraging, make sure you report, make sure you become good friends with police," she said.
Anyone with information about the shooting is asked to call the Homicide Squad at 631-852-6492 or anonymously to Suffolk County Crime Stoppers at 1-800-220-TIPS.
Greg Cergol contributed to this report.


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Click here for the slideshow. Last month we had a great day at the Women's Startup Lab in Menlo Park, workshopping online community building for founders. (See details here.) The questions these entrepreneurs asked got us off to a good start:
- "How do I reach people I don't know?"
- "Which tool can I use to bring together two communities and get them communicating?"
- "My community lives on Twitter but what if my end users are on Facebook?"
- "How do I combine my posts in two different languages?"
- "How do I maximize social networks to grow community, and where I do find the time for content creation?"
#Startup founders @wslab building community online through #socialmedia @AnastasiaAshman pic.twitter.com/WeeHkNwtFb — Tanya Monsef Bunger (@TMonsefBunger) May 13, 2014
idea from today's workshop: #community starts with you. #globalniche — GlobalNiche (@globalniche) May 13, 2014
It was wonderful to meet and get a peek into the businesses of founders Dedra Chamberlin (Cirrus Identity, for social identity management in the higher education space), Cynthia Litchi (she's launching Tejul, a social learning site for Latin American female artisans to teach each other), and Vicky Zhang of Fledgg (a company to connect young entrepreneurs and mentors globally).
#startup #entrepreneurs at @wslab building online #community! pic.twitter.com/ySd8TflyOl — GlobalNiche (@globalniche) May 13, 2014
@chelit @dedrachamberlin @TMonsefBunger great morning at @wslab work shopping online community to grow your #startup — Anastasia Ashman (@AnastasiaAshman) May 13, 2014
Cultural and personal sensitivity issues we covered in this workshop included how to strike a balance between one's CEO presence and personal presence online since "it's risky!" We also talked about optimizing your profiles at all the social media services and sites you're a member of. Cynthia Litchi mentioned that in Mexico, where she's from, professionals don't use LinkedIn because of security issues -- so if you want to connect with Mexican pro counterparts online, more closed settings are where you're going to find them. Thanks to Tanya Monsef Bunger, a coach at the Women's Startup Lab, for coleading this workshop and Ari Horie and everyone at the Innovation Lab for hosting.
I want to do more workshops with entrepreneurs about how they can use social media, online presence building, content creation, curation and content marketing and personal branding to grow community...
because entrepreneurs come to the table uniquely prepared to use their online presence to meet their goals.
They have a huge vision, they have distinct goals, they have content, they have a platform of some kind. And they are driven to make the most of their resources. And that's exactly what they need to combine in order to connect with their peers, their customers, mentors, advisors, investors.


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ECP will be closed Monday, April 20 and Friday, April 23, 2018.
The Extended Care Program is an after school program serving all registered students of St. Matthew Catholic School. Its purpose is to provide care for all the students as conveniently as possible in a safe, enriching environment filled with activities, fun programs, good friends, and loving staff members. We take pride in our program and in making sure children feel comfortable and welcome.
We are fortunate to have Mrs. Jenny Southlynn as our ECP Director. Jenny is very talented in the Arts and has taught numerous youth painting classes. She is a founding member of the Urbana School District's after school program and is the founder of The Boneyard Arts Festival.
ECP Hours: 2:30pm-5:30pm
ECP Phone: 217-359-0330
ECP E-Mail: firstname.lastname@example.com


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One thing to keep in mind when studying car reference points is these are just standard reference points and they may not be the exact match to your vehicle. Your vehicle may be shorter or larger than the vehicle used in these examples and so your vehicle reference points may be somewhat askew. They will still be very close to the standard, but you should always check the reference points for the vehicle you are driving and memorize them.
Side position reference points
Side position reference points allow drivers to estimate where the vehicle is located in relation to one of the curbs or pavement markings. Side position reference points can be used when you are driving and are choosing a lane position or when you are trying to park the vehicle.
Left headlight
When you need to park the vehicle on the left side of the street, your left headlight makes for a great reference point. Draw a visual line through the point where the left fender meets the hood of the vehicle. This point lines up with the curb/lane markings when the vehicle is just 3-6 inches away from the sideline.
Using the left headlight as a reference point works well when you need to:
- park the vehicle on the left side of the street
- position the vehicle for a left turn
- assume "lane position 2"
Center of the hood
The center of the hood is a great reference point that allows you to get very close to the right curb without actually hitting it. When the curb appears to intersect the center of the hood – you are in that sweet spot when your right wheels are just 3-6 inches away from it.
The center of the hood is a standard reference point on your vehicle when you need to:
- park the vehicle on the right side of the street
- position the vehicle for a right turn
- assume "lane position 3"
Reference points for the "STOP" line
When you are coming to a full stop before the stop line, you have to be able to judge the position of the front part of your vehicle. Side-view mirrors serve as great car reference points in such cases. The picture on the left demonstrates how a stop line would look next to your left side-view mirror if you stopped just before the line.
The picture on the left demonstrates how a stop line would look from your right side-view mirror if you stopped just before the line. In reality, you will not see the stop line from your right side window if you are standing in the right lane.
Rear end of the vehicle
Knowing the rear limit of the vehicle is important when you are backing up and parallel parking. Looking over your right and left shoulders through side windows should help you relate the position of the vehicle to the curb line.
The image on the left demonstrates a curb line when the vehicle's read end is positioned right next to the curb and the driver is looking over the left shoulder. Note: you can use the door handle as your reference point.
This image demonstrates how the curb line appears in the side window when the vehicle's rear end is positioned next to the curb and the driver is looking over the right shoulder.
If you are still wondering why you should learn your vehicle's reference points, consider these things:
- knowing the car reference points will allow you to get in and out of tight parking spots
- you can quickly establish standard reference points for ANY vehicle and feel comfortable behind the wheel even when you are driving a vehicle that is larger than the one you are accustomed to
- passing cyclists becomes much easier since you know your exact position in the lane and how it relates to the cyclist
- you can use these reference points for parking! Parallel parking is a breath when you know how to correctly position your vehicle and learning your vehicle's reference points is essential for that!


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One of the hardest parts of the sales process in most industries is closing the sale. In the wedding industry, once you get the lead, getting the appointment is typically the next logical step and (hopefully) takes a bit less work. However, when you get through an appointment it can be hard to walk away with the sale. WeddingWire Education Guru Alan Berg addressed this topic in his discussion of the "dreaded" price question.
Why do couples always ask about price first? They ask "how much" because they don't know what else to ask. It's not that they're price shoppers or that they can't afford your services; sometimes they just don't know where else to start the conversation or how much to expect. After all, everyone needs to know how much a product or service they want costs at some point and no one wants to overpay.
Remember that each couple you meet is trying to plan an event they have never planned before (for the most part). They're not always looking to haggle on price. Sometimes they just don't know enough about your business to differentiate it from the rest of the Pros out there. That's where closing the sale through education becomes a viable strategy.
Avoid the immediate "How much?" by educating your prospective clients about your offering in general as well as about your business! Think about the most important things a couple should know or might not have considered. Then think about how your business goes above and beyond to meet those needs. These are the talking points to use during your appointment!
You can also start the education before the interview with the following strategies:
- Identify pain points or add-ons a couple may encounter as they're booking their Pros and write a blog post about resolving them and making educated choices
- Link to that blog post and/or include similar information in your response to the initial inquiry
- Create an interesting graphic or infographic with the top things a couple should know when booking your business
At the end of the day, most people don't like feeling "sold" or taken advantage of. If you've had a bad sales experience in the past, remember how that made you feel and don't repeat it! Always put yourself in your prospect's shoes and think about your sales pitch from his or her perspective. Would you rather your potential Pro sell you on a package, or educate you on the decision to make sure you don't take any false steps? We bet it's the latter.
By educating prospective clients, you'll help them understand what they need to know to make the right decisions, what options they have based on their preferences, and how your wedding business can fit their needs!


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It's not surprising that passionate proponents of consensus decision-making…
Strangers on Delta Flight Caught Having Oral Sex in Their Seats: A 28-year-old man and a 48-year-old woman - who had just met while sitting next to each other on a Delta flight from Los Angeles to Detroit - face possible felony charges after the woman was caught in the act of giving oral sex to the male passenger.…Continue
Added by Johnny Robish on October 31, 2017 at 10:47pm — 18 Comments
Were i Herr Papadopoulos, Herr Manafort, Herr Gates even Herr Trump and Herr Pence -or a likely a couple dozen and more underlings and relatives- i'd be thinking about seeking asylum in Russia or elsewhere,, not i, but some might choose IsRealHell in Palestine.
Added by moki ikom on October 31, 2017 at 7:20pm — No Comments
Pam was a woman I met through my then wife as we struggled to support four kids in our new small custom home in an affluent Texas neighborhood. She had come from Arkansas to seek a new life in a rapidly growing Texas economy where employers trended toward the positive aspects of the "Sun Belt" and were relocating many of their…Continue
Added by Doc Vega on October 31, 2017 at 3:00pm — 14 Comments
After months of speculation that Il Capo, Donny T might have asked for help in eliminating his opposition Hillary "the hellion" Clinton from "Vlad the Impaler" Putin there appears to be truth to the rumors. Vlad has denied everything, and maintains that his nickname is not warranted. Unlike the historical Vlad, he prefers to slip his enemies a radioactive mickey.
The last five days have been interesting. On Friday we were given word that the Feds were about to put a…Continue
Added by Rodney Roe on October 31, 2017 at 1:14pm — 16 Comments
hi all. a difficult subj to write on, but am feeling like just want to get it "off my chest".
my last blog on this subj got a unusually high hundreds of hits. thx cyberspace for paying some attn to this crucial topic.
a lot can be written on this but am going to try to be brief.
the FBI is a critical organization for this country and its…Continue
Added by vzn on October 31, 2017 at 9:30am — 6 Comments
Re slavery:
Excerpt from Jefferson's draft of the Declaration of Independence:
"(H)e has waged cruel war against human nature itself, violating it's most sacred rights of life and liberty in the persons of a distant people who never offended him, captivating and carrying them into slavery in another hemispere, or to incure miserable death in their transportation hither. This piratical warfare, the opprobium of infidel powers, is the warfare of the Christian king of…
Added by Ron Powell on October 31, 2017 at 5:00am — 46 Comments
Added by Jonathan Wolfman on October 31, 2017 at 3:33am — 1 Comment
Study Finds People Who Use Marijuana Have More Sex: A new study involving more than 50,000 Americans found that people who use marijuana tend to have more sex than those who don't use the drug. What a bunch of bull! Why, after I read this story, I ran right out and smoked a joint and then asked a woman if she'd like to have sex with me and she said "absolutely not!"…Continue
Added by Johnny Robish on October 30, 2017 at 9:07pm — 9 Comments
This writer predicted months ago that the longer the investigation into Russian collusion over the Trump White House continued the more it would incriminate the DNC and the numerous players of the party who had a hand in the false accusations attempting to deter a Trump win in the 2016 elections. Instead it has backfired as new…Continue
Added by Doc Vega on October 30, 2017 at 2:30pm — 5 Comments
Turning and turning in the widening gyre
The falcon cannot hear the falconer;…
Added by mary gravitt on October 30, 2017 at 2:30pm — 13 Comments
The Proliferation of a Myth
It all began during the Obama administration as a troubled young man with a police record in his teens was summoned for questioning by a neighborhood security guard. There in the rain in a community troubled recently by a number of break ins and…Continue
Added by Doc Vega on October 30, 2017 at 1:00pm — 15 Comments
It's not surprising that passionate proponents of consensus decision-making…
Added by marshall bjohnson on October 30, 2017 at 6:41am — 39 Comments
I hereby make the following motion:
You're as welcome here as anywhere else. As a body, we ask that you don't over do it by straying from the topic...
You may respond to comments that are addressed to you, but we further request that you please stay within the bounds of reasonableness and rationality.
The motion has been placed before the body here present for consideration, discussion, and debate.
Is there a second?
According to…Continue
Added by Ron Powell on October 30, 2017 at 5:00am — 14 Comments
Added by Dicky Neely on October 29, 2017 at 11:53pm — 2 Comments
Man Dressed as Santa Shoots Four at Texas Halloween Party: Police say a Halloween party in Austin, Texas, erupted in violence after a man dressed as Santa Claus opened fire and wounded four people.
While I'm no detective, I'd say the…Continue
Added by Johnny Robish on October 29, 2017 at 8:07pm — 4 Comments
Spent the last week on the Island, with no innterTubes but with a bookstore. Recently saw Kurt Andersen flogging his latest book, "Fantasyland", which was on the shelf and the appointed companion to DB2 and stat text brought along. His interview had convinced me to do…Continue
Added by Robert Young on October 29, 2017 at 7:48pm — 3 Comments
Wisconsin Man Starts Drinking After Getting Locked in Beer Cooler: Police have cited a Marshfield, Wisconsin man who started drinking beer after becoming trapped in a Kwik Trip convenience store's walk-in beer cooler after sales clerks told him they couldn't sell him any alcohol. Oh come on, what's the problem? I mean, the guy dutifully refilled all the beer bottles while he was in there. Its simply a case of "when life hands you a well-stocked beer…Continue
Added by Johnny Robish on October 28, 2017 at 8:05pm — 1 Comment
Not really.
I'll check here in the morning.
Added by alsoknownas on October 28, 2017 at 3:08pm — 20 Comments
Once Again the Rose Fingered Titaness Paints the Sky
In Ancient Greek mythology there was a pantheon of gods and goddesses consisting of primordial gods, Gaia (Mother Earth) and Uranus (Father Sea). A second set of Gods – the first generation Titans - were giants of incredible strength; the sons and daughters of Gaia and Uranus.…Continue
Added by Rodney Roe on October 28, 2017 at 7:30am — 29 Comments
Added by Jonathan Wolfman on October 28, 2017 at 6:57am — 14 Comments


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Winston Byrd has not received any gifts yet
Inspired by former jazz trumpet greats as Dizzy Gillespie, Maynard Ferguson and others as early as in the seventh grade, Winston Byrd has already firmly established himself as a young rising force on the horizon in jazz to be reckoned with. This is simply attributed to after graduating from high school in 1991, instead of going on to New York University to study Clinical Psychology, Winston's personal belief in his talent made him boldly decide to devote his life to jazz. Attracted to and fascinated by the art of playing high notes on the trumpet, Winston diligently practiced until he became capable of easily playing and articulating extreme ranges of high notes into the stratosphere on trumpet.
Winston left home and went on the road to perform, record and tour with a wide-variety of highly acclaimed, Grammy winning, recording hit musical groups and artists, such as the R&B, Rock and Pop stars, the Stylistics, the Chi-Lites, Patti Austin, The Dells, The Grateful Dead, Blood, Sweat and Tears, Michael Bolton, Natalie Cole, Chaka Khan, Mary Wilson (of the Supremes), Usher and such hailed jazz artists such as Pieces Of A Dream, Claudio Roditi, Larry Coryell, James Moody, Paquito D' Rivera, Clark Terry (who was a surprise guest with Winston on his All-Star 31st birthday concert!) Lucky Peterson, Bernard Purdie, Diane Schurr, Shirley Horn, Chucho Valdes, David "Fathead" Newman, Abbey Lincoln, T.S. Monk and even comedian Don Rickles.
His first CD entitled, "Soul Searchin," which is available in stores, as well as on the Internet at: www.winstonbyrdmusic.com;. Trumpeter, Winston Byrd also has a number of his own compositions that have been recorded on T.S. Monk's (the great jazz pianist, Thelonious Monk's son) latest release entitled "Higher Ground" where Winston Byrd (former member) can also be heard. Currently Winston has continued performing, recording and touring with even more top musical artists and groups on the West Coast, such as recently with the Gerald Wilson Orchestra, the Frank Capp Juggernaut, the Buddy Collette Big Band, Steve March Torme, Eric Benet, The Don Ellis Reunion Big Band, Ernie Andrews, as well as also performing live on the L.A. based award shows such as the Grammys & the B.E.T. awards!!! Winston Byrd is truly an arising trumpet star whose moment of greatness is definitely at hand.


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And the Brooklynites map US tour
Oxford Collapse have announced the release of their fourth album, 'Bits', slated to hit shelves August 5 via Sub Pop.
The fourth album from the Brooklyn-based trio, 'Bits' follows 2006's 'Remember The Night Parties' and is the second release from the band on the Seattle imprint.
The band road-tested much of the new record while on their recent stint on the road with borough-buddies We Are Scientists, and recorded the album in chunks over the course of last year with Eric Emm at the Brothers Studios, and Chad Matheny in "spaces all over town".
The track listing for 'Bits' is:
'Electric Arc'
'The Birthday Wars'
'Young Love Delivers'
'Back of the Yards'
'A Wedding'
'For the Winter Coats'
'Men and Their Idea'
'John Blood'
'I Hate Nobody'
Meanwhile, they will support the album's release with a US tour. The dates are:
Pittsburgh, PA Gooski's (June 10)
Bloomington, IN Bear's Place (11)
Chicago, IL Empty Bottle (12)
Madison, WI Terrace (13)
Omaha, NB The Waiting Room (14)
Denver, CO Hi Dive (15)
Missoula, MT The Badlander (17)
Seattle, WA Nectar Lounge (18)
Portland, OR Holocene (19)
San Francisco, CA The Independent (21)
Los Angeles, CA Roxy Theatre (22)
San Diego, CA Casbah (23)
Phoenix, AZ The Rhythm Room (24)
Austin, TX The Mohawk (26)
New Orleans, LA One Eyed Jacks (27)
Washington DC Black Cat Backstage (30)
Boston, MA TT the Bears (July 1)
Brooklyn, NY Southpaw (2)
Philadelphia, PA Johnny Brenda's (29)
Brooklyn, NY Music Hall of Williamsburg (31)
New York, NY Bowery Ballroom (August 1)
Columbus, OH The Basement (3)
Detroit, MI Magic Stick (4)
Indianapolis, IN The Music Mill (5)
Minneapolis, MN Varsity Theater (7)
Cleveland, OH Grog Shop (9)
–By our New York staff.


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Legendary Pictures has picked up Martin Helgeland's fantasy script "Slayer" says Heat Vision.
The story follows a modern-day doctor dealing with the loss of his father and uncovering the truth about his dad's true identity – a dragonslayer. The doctor soon learns he is next in line.
Alex Garcia and Alex Hedlund will produce. Martin's father, Brian Helgeland, will serve as executive producer.


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I mentioned before on this blog that I learned to senegalese twist last year. It's my go to style whenever I have time to do it. I can wear my twist for 1-3 months depending on how well I care for them. My hair grows like crazy when I wear these twist. There are a lot of videos on youtube to teach you how to DIY. This style cost $90-$150 in the salon. It cost me $20-$25 (cost of hair, 11 packs) to do it myself. 🙂 I always wash and deep condition my hair before I install my twist. I use a leave on conditioner and oil my scalp. Continue reading
Ombre'20 Apr
Definition: The hair world borrowed the term 'Ombre' from the french word meaning shaded or shading. Ombre hair color is generally darker at the roots through the mid-shaft and then gradually gets lighter from the mid-shaft to the ends. It is usually achieved using a balayage technique and is ideal for clients looking for a low-maintenance style. -About.com
Video: DIY (Do It Yourself) Ombre
How I learned to Senegalese Twist.15 Apr
When I did my bog chop, I was living overseas in South Korea. I had NOOOO idea what I was going to do with my hair. Looking back, I wish I had more products and knew the info I now know bc I could have rocked my TWA (teeny weeny afro) and really did the dang thang. BUT, for me, resources were few in Busan Korea. Thankfully, I met a Nigerian woman who was braiding hair outside a military base in Daegu and for 7 months she kept my hair in Senegalse Twist.
When I returned to the U.S., I wanted to keep getting my twist but the prices over here were so high compared to what I'd gotten used to paying so I had to either learn to do it myself or "bite the bullet." I chose to learn…I watched this video for about an hour and got down to business. The first time it took me two and a half days (on and off). The second time, just 6 hours.


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Happy Birthday! Brotha Malik,
I think the best gifts I can give to you would be this.
You were father to six baby girls, husband to Dr. Betty Shabazz, minister to Muhammad Mosque # 7 in Harlem, public orator, Human rights leader and an evolved, redeemed, Brotha!
You were a MAN!
I would suggest folks run out and buy "Remembering Malcolm" and place it alongside their copies of The Autobiography of Malcolm X. It that tome, Minister Benjamin Karim, Malcolm's assistant minister of Mosque # 7) tells us about you as a Minister, how you preached, how you taught your congregation, how you pastored your Mosque. It shows a practical, pragmatic, patient approach that enshewed extremism. This is vastly different than the public speeches we see on you tube.
It also dispelled some of the myths. You remember the scene in the film Malcolm X where after speaking at Harvard or Boston College, The young white female student, asks Brotha Malcolm what she could do to help? And he said " Nothing!" Minister Benjamin said that Malcolm always regretted that because if he had been alone he would have had a cup of coffee with her and gave her ideas on how to help but he was with his group of young ministers and he put on a show.
Malcolm didn't go to Mecca to learn about the Universal Religion of Al-Islam, he knew it quite well beforehand. He was able to embrace it fully and demonstrate it after his Hajj though. He also had a real relationship with Dr. King. They kids used to play together. Minister Malcolm would come up to Mrs. King and say tell Martin, that I'm going to preach out against this hard this week and he should preach the opposite so that we can get something done. They'll think Martin's being sensible. They were not at odds they way people think.
Again, The Orthodox Islamic Community had approached Malcolm on several occasions, he had spoken with them, shared views, he knew them and they wanted him. They gave him a check for five million dollars to leave the Nation of Islam and start an Orthodox Mosque years before his public falling out with his mentor The Honorable Elijah Muhammad. He showed that check to his ministers and to John Henrik Clarke and mailed it back to them. He was loyal but he knew when someone was trying to co-opt him. He was friends with Wallace Muhammed, eventual leader of the Nation of Islam, who was repeatedly sanctioned by the NOI for failing to teach the NOI doctrines and teaching Orthodox Islam in his mosque. He taught Malcolm alot of Al-Islam however one must remember Malcolm met people from all over the world and Malcolm read voraciously. He wasn't in the dark about Al-Islam. He had been at one time but he grew and he would later criticize the Orthodox Community in his later speeches for not making a better effort to share the truth in the Black community and leaving us prey to any charlatan that came along. He also criticized Elijah Muhammed who he said could have brought us into the true light of Al-Islam but instead continued to teach what are seen as heresies in Al-Islam. Malcolm said it! Check it out!
Remember Malcolm had been through Philly many times, this is home to the first and oldest Mosque in America and started by alot of Afrikan Amercans in 1899. It was an Orthodox community mosque.
So when Noble Drew Ali, both Wally Fards, and Elijah Muhammad came on the scene teaching doctrines Malcolm later rejected there had been a real orthodox community available that failed to reach out. The Moorish Science, Allah Temple and Nation of Islam filled the vacuum, with race pride and uplift, drug rehabiliation and prison in and outreach and an economic plan that's rivaled only by Marcus M. Garvey economic plan.
Happy Birthday Brother Malcolm Little X Shabazz, African, Afrikan American, Minister, Father, Husband, Activist, Brotha! We love you and Miss you!
Be Mindful! Be Prayerful! Be Careful!


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August 13, 2013
PEACE VILLAGEThe UCC hopes an intergenerational experience that advocates understanding of other religions will help promote peace.
United Church News
Maryland congregations provide community meals through Mission 4/1 Earth garden
Franklinton Center mortgage burning ceremony celebrates covenant
UCC immigration reform advocates support release of the 'Dream 9'
Newtown minister joins racial justice tour remembering MLK's March on Washington
UCC, OC Inc. await FCC vote on lower prison phone rates
UCC leaders, Native American churches come together for future work
Louise Wallace remembered as visionary for UCC women
UCC In the News
California church's 1,000 Cranes for Peace project commemorates bombings
91,000 new trees for Planet Earth
Illinois faith leaders emerge as key communicators on health law
Pennsylvania church assists hurricane recovery on Staten Island
Tennessee church reaches milestone
Minnesota church celebrates 150 years
New church clock dedicated in New York
Pennsylvania church hosts community Night Out
UCC minister will speak in Oregon on veterans' moral injuries, healing role of church congregations
Missouri church sign stirs debate
Oregon pastor: Climate change is a profound threat and requires immediate action
Arizona church volunteers operate growing food bank
New Hampshire church welcomes interim minister
Ecumenical & Interfaith
1,000 peace cranes take flight
Evangelical 'Messy Middle' is more accepting of gays
Presbyterians stir debate by rejecting popular new hymn
Gay couple may sue Church of England to wed in church
Sociologist of religion who mapped the American soul dies at 86
New State Department Faith Office
Pope Francis is unsettling and dividing the Catholic right
Local atheist group challenges prayer at city council meetings in California
What imams talk about during Eid
Israelis seethe over Palestinian prisoner release ahead of peace talks
Mystery priest hailed as an angel at accident scene steps forward


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Tuesday, March 29, 2011
Bunny in the pink
Labels: Bunny in the pink
5 comments:
Awe Lillian this is adorable. I just love a sweet bunny as this one. I like your pretty lace at hte bottom too! Very pretty.ReplyDelete
How cute is this bunny? Love the ears, and your layout is great. Yep, Gail is gonna love this card!!!ReplyDelete
Hugs Monnie
just adorable Lilli!! color is so pretty and image is very cute!!!!!ReplyDelete
This is truly an adorable card. The colors are even prettier in person. Lillian colors absolutely wonderful! I am the lucky recipient of this adorable card! Thank you Lillian!! Hugs, GailReplyDelete
I like the flat on this card...you know me and flat! Very cute Lillian!ReplyDelete
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How to Get Referrals
Referrals—otherwise known as getting someone you know to give you warm leads to new prospects—are valuable for salespeople in all industries. Yet it's incredible how few salespeople actively canvass for referrals.
Studies show that a salesperson is six times more likely to close a sale to a referred lead as opposed to a cold lead. That means you can generate six times as many sales if you focus on getting referrals than if you spent that time cold calling! A huge increase in sales and, as a bonus, less cold calling to do—what more can you ask for? And if you open your eyes, you'll see opportunities for referrals all around you.
Existing Customers
Your own customers are the easiest and friendliest referral source. In fact, if you treat them right, they may well go out and do some selling for you! That's when you get those wonderful windfall calls from someone's co-worker or uncle saying, "I hear you sell the best widgets in town. I'd like to buy 40 of them."
Don't wait for your customers to do all the work for you—pick up the phone and ask for the referral, or send them a referral request letter. It's a good idea anyway to check in with your customers a few weeks or months after the sale. You can ask them how they're enjoying the product, find out if they have any questions, and then pop the question: "Who else do you know who can benefit from this product, as you have?"
New Customers
Just after you've closed a sale with someone is the best time to get referrals from them, because they're excited about their new purchase. Some salespeople are nervous about asking for referrals at this point because they just want to get out of there in case the prospect changes their mind! Well, don't worry about this. Unless you've used high-pressure tactics to intimidate someone into buying (don't do this) your new customer is probably thrilled and enthusiastic. Hit them up now, while their energy is at its peak!
Prospects You Couldn't Close
If you pitch a prospect and they turn you down, don't just bolt out the door. Get a referral or two, and you'll have changed a loss into a win.
You're probably shaking your head and thinking, "That's crazy-talk. Why would a person who wouldn't even buy from me give me referrals?" A lot of sales fall through not because the prospect hates you or your product, but because they just aren't a good fit. In that case, it's the perfect opportunity to find out if they know someone who is a good fit. Don't assume the prospect hates you and your product—after all, you have nothing to lose by asking!
Everyone Else
Literally, anyone you meet under any circumstances can give you a referral. After all, the average person knows in excess of 250 other people. Do you really think that none of those hundreds of people are a good fit for your products? Of course not. Ask everyone—your dry-cleaner, your accountant, your neighbor, even the person standing behind you in the supermarket checkout. You'll be amazed at how many leads will drop into your lap as a result of a brief conversation.


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|An old building we found along the way
Last year, another of our road trip excursions included a trip to Great Basin National Park in Nevada. It was a few hours away and we had the opportunity to take some roads that we don't normally travel on which provided an interesting drive even though we discovered that there was absolutely no food options for hours. (We ended up backtracking to civilization to grab one last fast food meal before heading off into the unknown.) Because we've never visited before, we were unsure how secluded it would be, but we did discover that the visitor center at Great Basin has a great café with great food and ice cream options which we took advantage of.
|Inside Lehman Caves
The big draw at Great Basin is Lehman Caves. They offer two tours with different lengths: the Lodge Room (60 minutes) and the Grand Palace (90 minutes). We opted for the shorter one which was recommended for younger kids. The website stated the tours were for 5 years and up, so we double checked before booking the tour to see if younger kids could go too. They were very helpful on the phone and we learned that the age was more of a recommendation than a restriction. The two tours are pretty much the same except the Grand Palace included two more rooms. We didn't feel like our tour was lacking though and were happy we picked the Lodge Room tour. It was the perfect amount of cave for us.
|The result of the children's cave activity
Great Basin was a great place to visit with the family. The park rangers held free children's activities daily including a cave building activity that Jadon really enjoyed. It definitely helped him remember all the different names of the rock formations and he loved showing off his creation. We also attended another activity for pictographs where the kids got to learn and draw their own. There were less kids at this one so it was basically one-on-one time with the park ranger which was nice. Great Basin also boasts some of the darkest night skies because it's so secluded, but we never managed to attend any of their stargazing parties. Maybe next time around.
|Along the Alpine Lakes Loop Trail
|Stella Lake
The only real hike we went on was the Alpine Lakes Loop Trail which was only 2.7 miles long but it still proved too much for Isabelle. Andrew ended up taking her back to the car before reaching the lakes which was a good thing because we encountered torrential hail and rain on the way back. Apparently, the weather here is unpredictable and it isn't uncommon for this to happen which we didn't expect and were not prepared for. We ended up getting pelted by the rain while running from tree to tree to seek shelter in between the spurts of hail. We couldn't run down to the cars fast enough. In retrospect, it was an experience that was worthy of being told, but at the time it was pure pain and fear. Andrew and Isabelle on the other hand were enjoying the weather from the comfort of the car while eating snacks.
|A view of another lake through the storm
On our last day here, we went on the Mountain View Nature Trail (0.3 miles) which the kids really enjoyed. It's a self guided trail with a guide you can borrow from the visitor center. There were numbers through out with information that was semi-interesting. The kids loved it because it was themed for the junior park ranger and the kids took their jobs very seriously. Before leaving the visitor center, the kids were able to turn in their junior park ranger activity books they had gotten when we first got there. In exchange, they were presented with their junior park ranger badges and they took their oaths. It was so cute.
|Original entrance into Lehman Caves
We learned from the pictograph activity that there were pictographs in the park. We decided to look for them before heading home. It took us a little while to find, but we were able to find them right off the road along some hidden caves in the mountainside. We found two caves not too far apart with one being significantly larger. The caves were closed off though so we could only see from afar.
|Hiking another trail
We stayed here for two nights and we were definitely able to get our fill of Great Basin. We stayed at a reserved group camping site which was huge and secluded so we didn't have to worry about quiet hours. We did get rained out one night though which goes to show that this place really does have unpredictable weather. All in all, this trip was a good weekend getaway with educational elements. The kids enjoyed the activities and Andrew and I had fun visiting a low key national park.