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Florida home insurance is something that every homeowner needs to have. Not only will it give financial security if some sort of a disaster should happen — a fire or a flood, for example — but it can give you peace of mind in regards to the large investment that is your home. A home represents safety and security for a family. It gives them a place to come together and enjoy each other's company. Keeping this safe is something that you should try very hard to do and home insurance is the best way to do it.
What if your insurer refuses to give you discounts because some companies don't have non-smokers discounts? Then it's time to shop for an insurer who does unless you have something else to make your continued stay with such an insurance company worthwhile.
So what kinds of insurances are there? Well the most generic are fire insurance, home insurance and Thatched Insure. Fire insurance covers fire-related accidents. Let's say, for example, you were cooking and left the stove. The tank exploded and the next thing you know, your house is on fire. You can get your fire insurance's monetary cover and can get to start a new. You might have lost a lot, but getting a little money would be nice so you can at least do something. Next is home insurance. This is similar to fire, but with a wider coverage, and not just fire. The tsunami in Japan for example where houses have been destroyed by the strong influx of water will be covered by home insurance. Finally, property insurance which involves stuff that is inside the house.
In the current economic scenario, affording a holiday is no less than affording any other luxury. However, going on a holiday is essential to rejuvenate your body and mind. So the solution is finding pocket friendly holiday options. One such emerging trend is a holiday In the cutting edge age of technology, arranging a is literally a child's play. It's easy it's affordable, and you have a holiday. There is hardly anything more which could get better than this. The concept of a for a holiday is nothing more than exchanging the houses for a holiday. However, if you are a first timer then, it could be a little confusing. Hereby some tips are discussed to ease the concept easy for you.
The kicker of the whole story is that Coop left on a Tuesday and unbeknownst of the bright burning burner, Schultz had planned to stay at his lady's place for a couple of days that week. That stove burned bright for all of Tuesday, Wednesday, and some of Thursday before, luckily, Schultz needed to pull by the pad to pick up a clean pair of p.j.'s.
When you apply for any type of loan or credit, your credit is going to be checked. Whether or not you are approved is going to depend on your credit. Also, the rates, terms and loan amounts you receive once approved will also depend on your credit rating.
An essential factor to be considered while you opt for a home swap is to be ready to live in a stranger's home and allow the later to live in your home. You need to be comfortable with the concept of only then the entire process will be effective. Before you decide on the home swapping idea, you need to ensure that you review you home insurance policy and check for guest coverage clause. All this said and done, now you are ready to indulge into a holiday hone swap for your next vacation.
Today we want to focus on giving you a better understanding behind why comparison sites are so beneficial. When you're finished here you can start looking at the kinds of savings you can enjoy online. However, right now we need to help you learn how comparison sites works, and why they are considered one of the most popular searching techniques on the Internet today.
If you are unsure as to how to proceed with this, you could also approach building insurance advisers. They can guide on the ways to seek appropriate cover. In the eventuality of the building getting destroyed by fire, you could easily make a claim. You need not worry unnecessarily about damage to property.


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Your Local Supplier
Britannia Windows, Exmouth
75 Exeter Road, Exmouth, Devon, EX8 1QD
Call: 01395 224596
Britannia Windows – Exmouth
Our Exmouth showroom covers the East Devon area, from the city of Exeter down to the seaside town of Sidmouth.
We are a company that really cares about our customers and that is why, unlike many larger companies, we believe in having local showrooms where our customers have the opportunity to see our products and meet our team before deciding to buy.
We are located at 75 Exeter Road, not far from the town centre and the seafront. Our friendly team of staff are available Monday to Friday and are looking forward to chatting about your next project and how Britannia Windows can turn your house into the home you always dreamed of.
75 Exeter Road, Exmouth, Devon, EX8 1QD
Email: firstname.lastname@example.com
Whether you are looking for stylish, secure and energy efficient windows; want to make a statement with a bespoke front door; add space and light to your home with a stunning conservatory or transform your living space and garden with beautiful bi-fold doors. Visit our Exmouth showroom where our friendly and knowledgeable team are waiting to help you.
Britannia Windows, 75 Exeter Road, Exmouth, Devon, EX8 1QD
Call: 01395 224596 | Email: firstname.lastname@example.com
What Britannia Windows, Exmouth, Customers Say
75 Exeter Road, Exmouth, Devon, EX8 1QD
Email: firstname.lastname@example.com

subset: commoncrawl


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Hey, can you guys who are using the iOS RIVA client, and who have been using the betas, upgrade to 5.0.922 and see how it's doing for you on the RIVA front. While working on some other issues, some very useful discoveries were made and I think that the RIVA server should be smoother and more forgiving than it has been in the past. And I just want to make sure you don't notice any little issues before I go out with 5.1. It's unlikely that there will be any issues, since the person I was doing these changes for says it's way better for him now. But it's best to know before the release than afterwards.
Dean Roddey
Explorans limites defectum
Explorans limites defectum


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House Ag Committee Chair Conaway on Crop Insurance, Farm Bill02/13/2017
House Agriculture Chairman Mike Conaway wants to begin work on the next farm bill as soon as possible. Conaway says a strong safety-net has helped farmers and ranchers weather the recent downturn in the agriculture economy. He says crop insurance has played a vital role for agriculture, and has worked well for producers during the recent challenges…
"Well, it's an integral part, I don't think we could maintain the overall safety net without it. There is no replacement for crop insurance, it is working well. Farmers count on it, bankers count on it, implement dealers count on it, in a kind of a second-hand basis for farmers are able to us it properly. So, no crop insurance is vital to the safety net that we currently have in place, and I see no replacement for it in the future. The public/private partnership on the delivery side is key to its success, we've increased acres under crop insurance. It's working, and working well. And as you've seen me in the past, I've worked really hard to protect crop insurance in the past, and I'll continue to do that."
As for the next farm bill, he says the process is already underway…
"Sub-committees will each hold a couple of hearings in the next six to eight weeks, their area of purview on each of the sub-committees. We'll also do listening sessions around the country, I'm currently waiting on the committee budget, so I'll know how much money I have to work with so I know how many of these listening sessions I'm going to have.
But, I want to hear from not only the leadership from the various producer groups, going to hear from rank-and-file folks as well. So, we'll head around the country and listen to people and what's on their mind.
We're going to lay the ground work now to be able to pull the trigger on something whenever we can begin to look at floor time we may have. So, the committee will be ready when that floor time becomes available, over the next 17-18 months, and we can get this thing done on time."
When it comes to changes to the farm bill, he explains what lawmakers will be considering…
"Pretty consistent theme, is the milk program, it has some issues that we need to deal with, cotton, the STAX insurance program, that did not work, and then concerns with county ARC program payments, in that they've got a real differential between payments across county lines. Cotton will be the biggest big change, but the others will be more fine-tuning of the '14 farm bill, I don't anticipate wholesale sweeping changes like we did in '14 where we did away with direct payments and moved to these other programs. I think it will be more of a case of understanding how those programs worked and where they need to be made better on a going forward basis."
Conaway says he and his colleagues will be working to get the farm bill finished on time…
"Colin Peterson and I plan on getting this thing done before the current one expires on September 30 of '18. There's no reason not to, it's been 16 years since we did one of these on time, and there's no real good reason for the drama associated with expirations, or extensions, or short-term things like that. We just need to get our work done.
He and I are going to drive this process to get it done. We'll obviously need some help in the Senate with Pat Roberts and Debbie Stabenow, but for us in the House, we're going to work real hard to get this thing done on time."
Source: Rhonda Garrison, Southern Farm Network


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Hello, my name is Bobo. The veterinarians say I am around 1 to 2 years old. I am a little timid at first but I gain trust easily and quickly. I am very obedient and learn new things daily.
I love to play with the tennis ball and have my belly rubbed. I am not fixed or micro-chipped yet but I am up to date on all my shots. If you are my loving and forever home, please call Animal Crackers Pet Rescue at (949) 499-1988.


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Buying a home will probably be the biggest investment a person will ever make, so whether you are a first time home buyer or if this is your dream home to retire, let us help so you can find your home asap!
Browse through all our properties that we have for sale. You can view detailed information on each property, check out our slide shows and even virtual tours! Find the perfect home that meets all your needs!
Selling your home? Find how to get the most amount of money for your home and sell it in less time. Selling your home can be a stressful process, we can help make it a stress free and smooth transaction.


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Bobby Boswell on GUTS: The Video
Way back in the early days of the blogging revolution (last fall), before Bobby Boswell launched his own Web site (in a blatant attempt to steal my readers), I brought you 1,470 words about his appearance on the old Nickelodeon program "GUTS," when he was crushed by a legendary female atthlete named Robin Rexroat. I e-mailed with the show's host, Mike O'Malley, to discuss the episode, and then finally, I found Robin Rexroat and had a nice chat about her domination of Young Bobby.
Because old blog stories never die, here, finally, is the video of Bobby's appearance on "GUTS." Watching it will require significant stamina and a high tolerance for childhood flashbacks. But watch it you must, because sources close to me believe that this will become an Internet phenomenon. At least on Big Soccer.
Dan Steinberg
June 11, 2007; 4:21 PM ET
Categories: D.C. United
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Owner Financed $200 per Month with $1,500 Down payment. Total purchase price: $19,900. This property sits between Willow Springs, MO, and Pomona, Mo just a short distance off US Hwy 63. The parcel is located on County Road 5360 only seven miles from Willow Springs and five miles from Pomona. The...Sold 5 Acres
Nice Home-Site With County
Pomona : Howell Co : Missouri
Ed Johnson : Homestead Crossing


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T. and I are celebrating a special occasion. We always seem to be; our good fortune calls for celebrating as frequently as possible. So on a rainy night in the Scottish capital, we head for The Cellar Door, a vaguely nautical-looking basement restaurant that promises especially good food.
Our table discussion turns to taxes and the morality of avoiding them. I say that nobody likes paying taxes and some taxes seem truly unfair, but as long as they are the law, then the only thing I can do is pay them, while working to change them (and any law that is unfair). I think this is part of the Discreet Traveler mentality. As I am a citizen of two countries, and resident in a third, being a "good citizen" means different things to me than perhaps to a homegrown patriot. I am a guest in this country. Paying taxes, and obeying laws, are how I participate in a nation, not owing allegiance. I don't expect to agree with policies for which I didn't vote. I honor the requirements of the country for as long as I choose to live here.
This is getting a bit heavy so we order some drinks. T. has Tiger beer from Singapore, while I have a white wine, a Gewürztraminer from Chile. Chilean is my favorite white, and the waitress says this particular wine is her favorite on their list. It is very good, as is the first course of The Cellar Door's "surprise menu," a smoked salmon spring roll. How nouvelle écossaise. This is followed by another Tiger and a ribeye steak for T., and a glass of merlot and the surprise main course for me. She finds the steak a little thin, but cooked the way she ordered it, and my lamb is out of this world. The greens are very good too, as are my mashed potatoes. T. thinks the excellent chips (fries) must be double fried, to get them so crispy on the outside and soft on the inside.
I am tempted to go for all three surprise courses, but opt instead for cheese with port for dessert. I have what my sister describes as a "cheese tooth." Even T., who has the sticky toffee pudding, can't resist the cheddar, which is called Isle of Mull--crumbly texture like a Cheshire cheese. All the cheeses are Scottish, and served with Arran oatcakes. Another principle of the Discreet Traveler: Whenever you have the opportunity, sample local.
The waitress brings the bill, not outrageous. and I note that she hasn't charged me for the glass of red wine. "Thank you!" she says. "Not everybody would have mentioned that."
Well, we were just talking about this, the Honest Abe mentality (Abraham Lincoln was said to have walked miles back to repay a shopkeeper who'd given him too many pennies).
"I can't help it," I say, and she agrees, she's the same way. If she'd overcharged me, I certainly would have complained. You can take the girl out of America, but...
Tomorrow I'm off to my day job, and T. is off to buy Isle of Mull cheddar cheese.


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Shakespeare's England has always had an impressive selection of hotels. But this year it's set to get even better with several of the region's hotels undertaking – or recently completing – major refurbishment programmes.
The former Holiday Inn in Stratford-upon-Avon has undergone a total makeover to transform it into a Crowne Plaza hotel. Set amid landscaped gardens beside the River Avon, the hotel has had all 259 bedrooms upgraded and enhanced, along with the public areas.
1st November 2016 saw The Eden Hotel Collection open the doors to its latest venture: Arden House. This intimate 10-bedroom boutique hotel is a sister property to the well-established The Arden Hotel next door – offering a relaxed home-from-home atmosphere with dedicated 'house hosts' who provide a 24-hour service.
Mallory Court will extend its offering by opening an Élan Spa in March. Boasting the latest energy efficiency and a state-of-the-art design in keeping with the main listed building, the luxury spa will have a separate entrance from the hotel to enhance the secluded setting.
Hotel du Vin has announced plans to open a new 48-bedroom hotel in Stratford-upon-Avon, which will be the chain's second property in the Midlands. Facilities will include a Bistro du Vin, meeting and private dining rooms, plus a banqueting room with capacity to seat 80.
Meanwhile, Macdonald Alveston Manor Hotel will reveal its brand new suite. It will also be undertaking a full refurbishment of the 11 bedrooms in the Manor House – recreating their original Tudor style features – and the 94 rooms in the modern extension.
So with all these exciting developments, there's never been a better time to plan a short break or longer holiday in Shakespeare's England. Even if you don't fancy staying in a hotel, we have lots of different types of places to stay, including family-run guesthouses, B&Bs and self-catering options.


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I would like to take this opportunity to tell my story about living with incontinence and hopefully show that with the correct treatment and support, bladder dysfunction need not be life limiting.
Every child grows at different rates, some walk sooner than others, some talk sooner than others, some learn to read sooner than others. As a young child, I went through all the normal stages of nappies and potty training and in the daytime soon gained bladder control, the only big difference with me was that I never had a single dry night. I wet the bed every night, waking up in cold soaking wet pyjamas and bedding. From an early age, over the years my parents took me to see many doctors who tried medicines, alarms and other methods to try to stop the bedwetting, but none of them worked.
For me, I suppose it was during my time at primary school that I first began to realise the effects that nocturnal enuresis, the medical term for bedwetting, would have on my life. Suddenly I found myself doing something that would continue for the next 30 years - making excuses.
As a child I joined the Cubs and they were often going on trips. I used to think of any excuse I could, not to go. One year it would be my gran is coming to stay, the next year it would be we were going on holiday. Every year it was the same problem, just a different excuse.
Even in secondary school, I would find the same problems would arise again and again. The School would arrange skiing trips, all my friends would be going and I was desperate to go but the fear of being found out and the stigma attached to being "the boy who wets the bed" was too much. I would use yet another excuse –too cold for me, I don't enjoy the snow. I even found a weekend job so that I could always use the excuse –I would love to, but I have to work.
With incontinence it feels like you are the only one and you have a secret you cannot share. But the fact is, this could not be further from the truth.
As I got older, the effect upon my life became greater, the bed-wetting problem was life limiting and affecting my quality of life more and more.
It was very hard to form any long term relationships. I would find myself having to make excuses all of the time and on the occasions that I was unable to make excuses, I would have to stay awake for fear of the embarrassment of wetting the bed. The odd night was manageable, but holidays were totally exhausting having to stay awake night after night. Even though I got engaged, I could never tell my girlfriend about my problem, so to me, hiding my problem was even more important than getting married.
Even my career choices have been dictated by my nocturnal enuresis. Training courses that would mean staying awake at night and working away were impossible.
Over the years, I saw many doctors and tried many different treatments but thinking back, I can never recall seeing a Urologist, especially during the earlier years.
How many people when they go to their doctor are asked about their bladder health? During routine visits the GP will often enquire about smoking, drinking, weight etc, but never do they ask about bladder health. So we end up with the situation where doctors don't ask, and patients don't tell.
My life suddenly changed about seven years ago when I sought the help of the Specialist Female and Restorative Urology Department at University College London Hospital (UCLH) who are world renowned in dealing with complex urological cases. After carrying out a number of tests, it was agreed that the best solution would be to carry out a series of operations to combat the problem. Initially an artificial urinary sphincter was implanted. After this had all healed and was functioning correctly, I was admitted into hospital for the second stage, which involved carrying out bladder augmentation to increase the size of the bladder and carrying out a Mitrofanoff procedure. Surgery went very well with no complications and after just a week in hospital I was allowed home.
A few weeks later it was back to UCLH to be taught how to care for the stoma, with the guidance of the specialist nurses, I soon managed to learn how to self-catheterise and carry out the bladder washouts.
From that day onwards I have been dry every night. The difference to my quality of life was huge. For the first time ever, instead of waking up cold and wet I was waking up warm and dry.
Even though I have undergone some quite complex surgery to correct my bladder control problems, due to the skill and dedication and support given by all at UCLH, having the Mitrofanoff procedure really has changed my life. For me the difference was not only at night but it also gave me a new lease of life, suddenly I found myself with more energy due to being able to have a proper, uninterrupted nights sleep.
Where previously I had begun to withdraw socially and emotionally, suddenly I found my confidence had returned and I was able to do so many things that I was not able to do before, nor ever thought would be possible.
The whole experience has inspired me to give something back and break down one of the last taboo subjects of the 21st Century. So in 2009, I spoke to Mr Jeremy Ockrim, Consultant Urologist at UCLH about setting up a charity to help others suffering from bladder dysfunction and between us we set up The Parnell Fund, which is now a charitable fund within UCLH Charities.
Although I had never run before, I have been able to run a number of marathons and have completed some pretty daunting challenges including:
I have also been able to go away and climb Mount Kilimanjaro safe in the knowledge that I would wake up warm and dry every morning, with no embarrassing
leakage. Waking up in the morning, in a tent on the side of a mountain looking down at the clouds where even the water in the drinking bottles has frozen during the night, may not be everybody's idea of fun but to me, it showed how far I had come in my journey in living with incontinence.
Even through the torrential rain we all had to endure half way up a mountain in sunny Greece ensured we all got very cold and wet during last year's Mount Olympus challenge. Hopefully this year's Mount Etna Challenge will be a little kinder to us.
But for me, most importantly, this year I have met a very special person who I am able to share my life with without fear or embarrassment of wetting the bed.
All of this would have been unimaginable if it was not for the Mitrofanoff procedure.
Suffering from Incontinence also made me realise not only just how many people suffer from bladder control problems, but also how many people are like me and never talk about their bladder control problems.
It is estimated 6 million people in the UK suffer some form of incontinence.
In 2010, nearly 4.8 million Australians – comprising over a quarter of the Australian population aged 15 years or over, were living with incontinence.
In truth both of these figures will be much, much higher.
Urinary incontinence in men and women is a relatively common problem– regardless of their age, gender or cultural background.
Women are more likely to be incontinent than men – with the life stages of pregnancy and menopause a major contributing factor.
Often both men and women tend to ignore their symptoms and suffer in silence for many years, waiting on average 4 years before seeking help.
Sometimes masking a more serious underlying problem that can and should be treated.
But as common as incontinence is, men often appear to be more reluctant than women to seek medical help and advice.
The chances are that you have never met a man who admits to suffering from it. It's not exactly a topic men are anxious to discuss after a game of squash or a pint down the local pub.
We need to overcome the barriers of embarrassment and awkwardness and encourage people to discuss bladder issues, not only with health professionals but also friends and family.
My name is Jamie Shannon and this is my journey.
I am a male who is 31 years old and have now had my Mitrofanoff for about 15 years. Here is just a brief outline of what I had done.
My problems began at birth. I was born with Epispadias, a rare condition which not many people have. I had a lot of reconstruction work done and spent a lot of time in and out of hospital. I don't really remember what I was like when I was younger, but I could not stop wetting myself till I was 10 years old. As you can imagine was not easy to deal with at the time. This is when I had a artificial sphincter put in to help keep me dry. It worked and was great, but then after a few years the complete opposite happened where I struggled to empty my bladder and then started to suffer lots of infections. I tried cathing urethrally and that was a complete nightmare and did not change the rate of infections.
Then I had the procedure that has saved my life, the Mitrofanoff. It has really saved my life. My life is not perfect but the Mitrofanoff has allowed me to live a normal life, go out and have a social life. I work full time in a office and have had the same job for 12 years. Since the operation I have not had to have any redo's or revision's and am very happy with everything. Infections are down to for a year rather than for a month. I am not a very active person but that's not to do with anything other than pure laziness.
The hardest thing that I have found about it all is the mental side. In 2011 I decided to do some research which is when I stumbled across Mitrofanoff Support (again another that has saved my life for a different reason). It was the bravest thing I had ever done and decided to join up. Up and till this point I had never met anybody like me and felt so alone. I was a very shy and quiet person who hated talking about it. I always thought I would be bullied and that people would judge me which now I know is far from the truth.
Mitrofanoff Support does a great job of bringing people together and holds some of the best events, getting to meet people and share your stories. I would even go as far to say some of my best friends.
The year is now 2013, I am so much happier as a person, feel more confident and not so embarrassed anymore. I help out at Mitrofanoff Support events and this year have become a Organisational Committee Member. A place where I can make a difference and help people the same as me. I would even say it's hard to shut me up about it. I would love people to know you are not alone and now there are plenty of people who can help you with the pros and cons, which I did not get when I was younger. People have Mitrofanoff for different reason and hopefully some of you can relate to my story.
Mitrofanoff Support Australia
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On the final day of the legislative session in Missouri, House and Senate lawmakers reached agreement on a lengthy bill that includes several provisions of interest to truck drivers. The measure – SB930 – now moves to Gov. Matt Blunt's desk.
A conference committee made up of select members from both chambers endorsed legislation that would prohibit large trucks from traveling in the far left lane on certain roads through the metro areas of Kansas City and St. Louis.
Trucks with a registered gross weight in excess of 48,000 pounds would be prohibited from driving in the far left lane of "urbanized" roadways that have at least three lanes of traffic in each direction.
State law enacted a year ago already prohibits trucks heavier than 24,000 pounds from driving in the left-most lane of Interstate 70 within three miles of the intersection with state Route 370 in St. Charles County.
Advocates for keeping trucks out of the far left lane say it makes roadways safer for all travelers.
A Missouri-based truckers group with 160,000 members nationwide contends that sentiment couldn't be further from the truth. An official with the Owner-Operator Independent Drivers Association said the group opposes lane restrictions for any class of vehicle.
"Adopting lane restrictions is an ill-advised step to take," said OOIDA Executive Vice President Todd Spencer. "Such restrictions invariably cause more problems than they fix.
"When you start restricting vehicles to certain lanes, you end up with more vehicles tailgating and making unsafe passing maneuvers in all lanes. This isn't good for congestion or highway safety.
"Lane restrictions simply discourage smart, safe driving practices."
Spencer said it's regrettable that lawmakers saw fit to make the change.
"This is an example where politics trumped safety and common sense," Spencer said.
In addition, Spencer pointed out that Missouri law already has restrictions to keep all traffic to the right except to pass.
Another provision of note attached to the bill is an incentive to reduce idling. The maximum gross vehicle weight limit and axle weight limit for large trucks equipped with idle-reduction technology would be increased. The bill would authorize affected trucks to weigh up to an additional 400 pounds.
The bill would also allow local enforcement of truck rules and prohibit certain indemnification agreements.
The procedure for conducting roadside inspections for large trucks also would be revised. A program would be set up to certify local law enforcement officers to enforce commercial motor vehicle laws.
Certified law enforcement officers could conduct random roadside examinations or inspections to determine compliance with the CMV weight and size limit laws. Officers who are not certified still could pull over large trucks if there was a "visible external safety defect."
Indemnity agreements would be prohibited in motor carrier transportation contracts that claim "to indemnify a party against loss from negligence or intentional acts void and unenforceable."
A separate provision in the bill is intended to keep repeat drunken drivers from driving drunk. In order to get back behind the wheel, ignition interlock devices would be required to be installed on repeat offenders' vehicles.
Interlocks are hooked up to the ignition of vehicles. Once such a device is installed, a driver must blow into a mouthpiece, which measures the amount of alcohol on a person's breath. If the driver blows clean, the car will then start; if not, it won't budge.
In addition, the devices often require drivers to re-blow in the machine after a designated period of time, to ensure that they have not convinced someone else to blow into the mouthpiece for them, or that they haven't been drinking since getting behind the wheel.
To view other legislative activities of interest for Missouri in 2008, click here.
– By Keith Goble, state legislative editor


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After 17 years of hard work by Western Center advocates, California has repealed the state law which banned food and basic needs assistance to people who had a prior drug-felony conviction.
Rosie was born in Southern California to an abusive father and a mother with severe mental illness. She was given narcotics as a young child by a relative charged with caring for her, but instead robbed her of her innocence. He was later arrested for the child pornography photos he took of Rosie. By the time she was 12, Rosie was on the streets, on her way to a life of crime and addiction. With no adult to care for her, Rosie was in and out of the juvenile justice system, never receiving the mental health care she desperately needed.
For several years, Rosie waded in and out of police custody and abusive relationships. She couldn't raise her first two children and asked her sister to raise them. It was after the birth of her third son that something changed for Rosie. "One day, I saw my son look at me after my boyfriend beat me up and I could see the spirit leaving from his eyes," said Rosie. She reached out to our transitional housing partner, Starting Over, Inc., and has been sober since.
Still, even though complying with her probation and a commitment to sobriety, Rosie couldn't make it out of poverty. Because California employed the optional ban for people with a prior drug-related felony conviction, she was denied basic needs assistance, job training, and child care. Her and her son have lived off of $300 in cash assistance and anything extra she can pick up with odd jobs.
After decades of hard work by Western Center advocates, California has repealed the state law which banned food and basic needs assistance to people who had a prior drug-felony conviction. Approximately six thousand families will now experience hunger less often and will be supported in work training thanks to heartfelt testimonies like Rosie's in the State Legislature that lead to the overturn of the backward law. Today, Rosie holds her head high. She knows that things will get better, not only for her, but for others.


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Colleges and universities have always been focal points of change. The mixture of academic research, student activism, and institutional clout has allowed campus communities to promote widespread technical and social transformations. During the last few years, a few of these institutions have begun to lead in an entirely new area—renewable energy. Just last September, the University of California system announced an 80 megawatt (MW) procurement contract for off-site solar photovoltaic (PV) electricity, enough to power almost 13,000 homes.
While this is the largest PPA on record for a university, it was not the first. Back in 2008, the University of Oklahoma signed an agreement with Oklahoma Gas & Electric to purchase 100 percent renewable electricity, spurring the development of a 44-turbine wind farm. Then, in 2012, Ohio State signed a 20-year power purchase agreement (PPA) for 50 MW of wind power. This year, George Washington University and American University teamed up, along with George Washington University Hospital, to secure 52 MW of solar PV from Duke Energy Renewables. Meanwhile, many campuses have installed significant on-site resources. Three universities in particular—Arizona State, Rutgers, and Mount Saint Mary's—have installed over 57 MW of solar PV combined; enough to power over 9,000 U.S. homes.
1. Renewables are a Good Deal
Recent transactions highlight just how competitive renewable power can be. Ohio State University estimated its wind transaction would save the university $1 million dollars in the first year alone. Similarly, American University says that its renewable energy contract "provides fixed pricing for solar energy at a lower total price than current power solutions." When you start to look at the recent, all-time-low wind and solar prices per kilowatt-hour (kWh), it's easy to see how these deals can be cost-effective.
In 2013, new wind projects in the U.S. had an average wholesale price of just $0.025/kWh. Meanwhile, recent wholesale solar PPAs have reached $0.05/kWh or lower. Add in the fact that these deals allow buyers to lock in low prices for 20 years or more, and the savings really start to add up. Ultimately, you don't need an Ivy League endowment to buy a lot of renewable energy—many of the leaders are public universities that have discovered it's a sound economic investment.
2. Top-Down Leadership
Since 2007, the American Colleges and Universities Presidents' Climate Commitment has encouraged almost 700 institutions of higher education to commit to achieving carbon neutrality within a defined timeframe. These commitments to reach carbon neutrality, sometimes by 2025 or earlier, are frequently cited as key components of a university's decision to purchase renewable energy. In fact, a few small colleges have already achieved carbon neutrality, most recently Colby College in Maine.
3. Bottom-Up Demand
Colleges and universities are also responding to a growing demand from their key stakeholders. Students and faculty around the country are already campaigning for increasing university sustainability programs, and these topics are also on the minds of prospective students. In a 2014 Princeton Review survey of student applicants, "61% said having information about a college's commitment to the environment would impact their decision to apply to or attend a school." Information about campuses' sustainability track records, provided through programs such as AASHE's STARS initiative, have brought additional transparency to these efforts and allowed for holistic sustainability rankings. Moreover, for both faculty and students, these renewables projects open up research opportunities. This was particularly true for Ohio State University, which employed over 400 energy researchers in 2013.
At RMI, we've shown time and again that renewable energy is held back not by technical limitations but rather by societal inertia, outdated regulations, and institutional barriers. Universities, by design, are hubs for experimentation and pushing beyond the norms of the day. As Dr. George Basile from Arizona State University observed, "When society doesn't know how to do something, universities are where you go to solve those problems." In addition, universities' relatively stable growth, long-term ownership of facilities, and intellectual atmosphere provide an ideal testing ground for new approaches.
For these reasons, RMI is engaging with universities through a number of programs, including:
However, more importantly, universities matter because they are educating the future leaders and members of our society. Integrating energy efficiency and renewables into a school's culture exposes each wave of students to more efficient and sustainable processes, systems and behaviors. That's social change. That's legacy. That's scale.
At the end of the day, whether you're a student, professor or alumnus, it looks as though universities still have plenty left to teach us.
Image courtesy of Shutterstock.com
The other important factor affecting universities' and colleges' uptake of sustainability initiatives is that their campus structure allow them to act as integrated microcosms with centralized decision-making authority that spans energy sourcing, food sourcing, building and grounds construction and maintenance, transportation, water usage and treatment, and various other influential sustainability decisions. With sole-source contracts for food services, lawn and grounds maintenance, etc., targeted influence of a few decision-makers can affect widespread change throughout the entire campus more easily than similarly widespread results could be achieved in the broader community, where each individual property owner / manager must be convinced and brought on board. For that reason, it's great to see the work that RMI is doing with large real estate holders: There is a great return on the effort invested to convince the decision-makers in those organizations.
What ever changes we are going to make, we need to hurry up and make them. I think where possible, Colleges and Universities should adopt the use of solar power and other renewable energy sources. I know you're not dummies, so I'm sure you have had these debates, but time is running out.
RMI Outlet, Rocky Mountain Institute's blog, explores topics critical to RMI's mission to drive the efficient and restorative use of resources.


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Robinair AC1234-6 AC Machine for 1234YF Refrigerant with Identifier, Printer, and Free Power Probe Temp Kit
Free Power Probe Remote Reading Temp Kit! Robinair SAE-Standard ACS Machine part #:ROB-AC1234-6 • The AC1234-6 is the only product available today that fully meets SAE\'s requirement for recovering, recycling and recharging vehicle A/C systems that use the new R1234YF refrigerant. • The AC1234-6 is a fully automatic ACS machine that comes with an integrated refrigerant identifier that samples the refrigerant in the vehicle\'s A/C system prior to recovery, is a highly accurate and reliable machine, and can service both standard and hybrid vehicle A/C systems. • Services both standard and hybrid vehicle A/C systems. • Fully automatic operation — program for service, walk away and return when complete. • 95% recovery efficiency • Programmable vacuum operation helps get to the necessary vacuum level in any condition quickly. • System leak test to identify gross leaks in a system. • Charge accuracy within +/- ½ oz. • Automatic oil drain shows how much oil was removed from the system during service. • Automatic air purge removes the potential of air accumulating in the internal tank. • Integrated refrigerant identifier (meets SAE J2927) • Liquid refrigerant flushing function can flush A/C systems or individual components using liquid refrigerant instead of harsh chemicals. • Automatic internal tank refill. No stopping to fill the internal tank during service, or before service. • 1/4 Color VGA display. Large easy to read graphical display • On-board printer capable of printing vehicle service report slips to present to customers. • Specifications • Operating voltage - 103-127V, 60Hz AC • 100mm, EN837-1 pressure gauges • Alpha-numeric keypad with service function keys • 1.5 cfm dual stage vacuum pump1/3 hp hermetic compressor • 22 lb. ASME internal storage tank • USB, SD, and RJ45 I/O ports • 8 ft. SAE J2888 service hoses with 1234YF high and low side system couplers • Meets all requirements of SAE J2843 Weight: 251.00 lbs.
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The African Grey must be the best known parrot. Undoubtedly because of it's uncanny ability to mimic speech and sounds so efficiently. They are extremely intelligent and forever alert. A hand-fed grey baby would certainly make any bird lovers heart melt.
We have the smaller Timneh Grey as well as the grand Cameroon Grey.
Janet Ford--owner
Fax: (616)266-2162
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Mix together Mexican warmth, something splashy for the kiddies and a special extra in each of our resort choices and you have the makings of a knockout family vacation.
taheima wellness resort & spaIt's a great twist on the mainstream vacation: The newest all-inclusive resort in Riviera Nayarit promises a holistic, multi-sensory experience with healthy gourmet dining and a full roster of daily activities for young and old. This is an all-suite property—accommodations on the bottom level have private plunge pools—that presents a "wellness passport" to each guest upon arrival with activity suggestions based on their needs. The choices here are enriching, fun and plentiful. There's yoga, zumba, Pilates and hip hop classes, as well as special activities like chi gong, tai chi and Labyrinth Meditation. The resort's Wizzards program offers kids workshops in photography, theater, collage, biking, yoga, croquet and golf, as well as cooking lessons. Taheima also has a Learning Center that features classes in pottery and painting, workshops on healthy cooking and nutrition, herbology and spirituality chats, lectures on anti-aging and self-preservation, a "Brain Gym" and a ton of other choices.
Clients can choose any number of suites, from a deluxe studio to a 3-story penthouse with full-sized Bosch-equipped kitchen, and each floor of the resort comes with personal butler service. Restaurant menus, from Mexican to Pan-Asian, have been designed by none other than the president of l'Académie Culinaire de France. Additionally, guests get priority privileges at the 18-hole El Tigre Golf Course and its installations.
The resort's introductory offer, good through Dec. 31, starts at $162 pp, per night with a 3-night minimum and comes with a daily massage and a free stay for kids 12 and under.
boca de iguanas Just 40 minutes north of Manzanillo, in pretty Costalegre, eco-haven Boca de Iguanas offers an expert combination of modern amenities and jungle chic, with just 10 suites with air conditioning and TV, ensconced in lush surroundings facing the beach plus two beach cabanas—with no TV, air conditioning or phones for those who really want to "rough it"—right on the sand. Regardless of clients' choice, accommodations are rich in details and Mexican touches: 400-thread-count Egyptian cotton linens, outdoor rain showers, all-natural amenities; some come with hand-woven hammocks and all with outdoor terraces. The resort is known for its attentive and friendly staff, not to mention a very low-key, stress-free and nature-rich vacation experience—ideal for non-fussy clients.
The recycling-friendly hideaway is all about going back to basics, with nature letting loose around you and large iguanas enjoying the sun from tree branches. The heart of the resort, in fact, is the mangrove system that thrives through the property and is home to many animal species. Spend the day on a boogie board, rappelling down a 50-ft. natural rock-climbing wall, kayaking along the waterfront or setting up a game of beach soccer—plus there's that enticing free-form infinity pool. When the sun goes down, there's always a chance to enjoy a bonfire on the beach, right under the stars. During the winter, guests can help release protected baby sea turtles out to sea. Rates start at $100 per night Oct. 15-Dec. 17; two children 8 and under stay free with their parents.
sandos caracol eco resort & spa Clients traveling with kids to the Riviera Maya can't go wrong at a place that has its own aquatic area with no less than 17 water slides, "raining" palm trees and water spouts throughout. Little ones who are enrolled in the resort's kids club get daily visits to the Aqua Park under the supervision of the Sandos Animation staff as part of the all-inclusive resort's offerings. Sandos also has a baby club for 2 to 3-year-olds.
The all-inclusive resort is packed with activities for guests of all ages, including a complimentary snorkeling tour of underwater caves and an eco-excursion that uncovers the diverse plant and animal life around the property, including curative trees, cormorants, donkeys and peacocks. Sandos has an onsite veterinarian that cares for the critters. These offerings are part of Sandos Caracol's commitment to the life surrounding it. As a result, the resort is well on its way to becoming one of Mexico's most environmentally conscious properties through various preservation and conservation initiatives, appointing themselves—according to director of sales and marketing Jacques de Paep—"full-fledged allies of the environment." As such, Sandos involves guests in its efforts through eco-workshops for children, designated wildlife areas, a Mayan Bee Project to preserve beehives and help new ones form, and keeping felines on the property healthy and safe through its Coco's Cat Rescue. The resort has also established similar programs for marine turtles, iguanas and other animals, and all guests are invited to learn about these initiatives during their stay. Rates start at $84 pp per night dbl.


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There are so many wonderful and breathtaking things to explore in Aeoul, but I've realized that while traveling in general, one should never set their expectations too high. As someone who'll soon be able to say that she's visited all of the countries she's dreamt of seeing since she was a child (Korea being one, Japan the other) I'm working on perfecting this whole "reasonable expectations" thing. I bring this up because I have accounts to share about my two recent adventures–to the Hello Kitty Cafe and to Seoul Comic World. Some aspects of my adventures were surprisingly amazing, while others, not so much. Let me elaborate ^^
The Hello Kitty Cafe is a cotton candy-hued building nestled away in a hilly side street in Hongdae. I've been there twice–on a Saturday and Sunday–and as one might expect, the experience varied drastically depending on the day. It's a sought after destination for tourists and kawaii girls no matter what day, but if you show up on a Saturday, anticipate that it could be a madhouse.
As for the decor, it's absolutely everything a Hello Kitty Fan could want. Kitty tables and chairs, cute kitty merch and memorabilia, and even the bathrooms are adorable.
As for the cafe's culinary offerings–they're as cute as you could imagine–kitty cakes, kitty waffles, and finely stenciled kitty lattes.
Within 15 seconds of entering the place you'll get drunk on its cuteness, believe me, but that being said, don't allow yourself to believe you've been swept away into a ideal kawaii wonderland–it's ultimately a busy cafe, and an unfortunately understaffed one at that. It seemed like 100% of the staff were serving up drinks and pastries behind the counter, leaving trashcans overflowing, tables unclean, and spilled drinks on counters and floors going unnoticed for the duration of our stay.
As for food and drinks–choose wisely. As a novelty destination, the main draw of the Hello Kitty Cafe is its cuteness, not its high quality food. The coffees and frozen yogurt are fine, not amazing, but no major complaints there. But my tiramisu tasted purely like warm egg yolks, so much so that it was inedible, and my friend abandoned her chocolate cake after one bite.
Complaints aside, I actually would still go to the Hello Kitty Cafe again, but I'd just go on a low-traffic day and stick to the drink menu. It's cute as hell when customers haven't made a mess of the place, and the cafe definitely offers some adorable photo ops.
Though when it comes to good photo ops, nothing really beats Comic World. Comic World is Korea's largest comic and anime convention–it's a fun and colorful cosplay wonderland, full of skilled artists offering beautiful fan merch and comics, and all sorts of contests and programming that wouldn't have been lost on me if I knew more Korean.
To compare Comic World to anime cons in the states, let me first explain that the cosplay scene in Korea is rather small compared to the states. And while Comic World is the largest anime con in Korea, you can scale the entire con floor in maybe an hour, if even.
Although, while comic world may be comparatively small in size, the fact that the event happens about every month is a fair trade-off (once a month in Seoul, about once every three months in Busan). This frequency makes it easier to form friendships and avoid the brutal end-of-con-depression that occurs when you attend a con that happens simply once a year.
I didn't cosplay this first time around, I just coordinated a goth lolita ensemble. They try to keep things more orderly than at a US con–they actually have cosplayers register, and have designated dressing and makeup rooms–so I wanted to feel out the situation first.
When I look at Comic World in comparison to other anime cons I've attended, the two things that really stuck out to me were the wide array of yaoi fan comics, and the large amount of incredibly well-executed crossdress and crossplay. Badass high school girls dressed as brooding, stylish boys were undoubtedly the star of the show at this
And might I just mention that language barriers aren't really a thing here. As with any con, everyone wants to have fun and take pictures with everyone–just make sure to be polite about it. Though, it goes without saying that you'll have a lot more fun at the con if you at least know a little bit of Korean.
I'll be attending Comic World again next month, and I intend to a cover a new aspectnext time around–feature more of the artists and offer a glimpse of some of the jaw-dropping yaoi that seriously dominates the convention hall (Loki and Thor? Woah…). Not to mention, I should have my two cosplays ready to roll pretty soon. I'm not sure if I mentioned what the next one will be yet, but if not, here's a hint…
So what do you think about Comic World and the Hello Kitty Cafe? Would you make a trip to either of them if you came to Seoul? Leave any comments or feedback below ^_^


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Feel like an at home spa with this Oh so purely simple and sweetly sublime – Bella's Virgin Coconut Sugar Scrub is… just divine! When you first breathe in the perfectly balanced aroma of delicious coconut, you will know this is the real thing – no overpowering fake coconut "smell" here! Just real, simple effective ingredients perfectly blended to deliver an unforgettable, totally craveable spa-like experience, whether at home or in a resort. The organic coconut oil melts delicately into the skin, gently delivering the refined, yet effective buffing power of the natural cane sugar, which goes to work on dry, rough tough skin, sloughing away to reveal glowing, hydrated silky-soft skin – hallelujah!
Directions for use in shower, bath or spa treatment: Scoop between fingers, apply to wet skin and gently massage in a circular motion, focusing on rough areas. Rinse thoroughly and pat dry.
WARNINGS: Avoid use on any cuts or delicate areas. Do not exfoliate if skin is sunburned or irritated for any reason. Be careful not to fall on wet or slippery areas caused by using the scrub. Avoid using all Body Polishes on the face.
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A couple days after she left, I was already missing her when she text me and asked if I would like to collaborate with her on upcoming products with my hand lettering. Of course, I readily accepted her offer! She gave me a list of words and phrases to letter and we made plans for products to come. Soon after, she began to release the first of the products and it is a thrill to see my lettering on her designs! It truly is a dream come true!
We discussed a lot of ways the show could go and decided we should continue the meaningful work that Cori puts in to her Faithful Life Club Challenge each month. What better topic to discuss than God's Word!? Of course, along the way, we'll share personal stories, revelations and sermon notes and I know Cori will share tips, as she always does, on resetting your life. The show will air each Wednesday morning and we are so excited for it to premiere! We're looking forward to connecting with listeners who want to focus on making Jesus the most important part of their life. If that's you, join us! We'll post a link on our social media and here as soon as the first show is up!
Meanwhile...we're making plans for a First Love Club Retreat! Join us in Nashville, Tennessee at my friend's gorgeous studio for a 12 hour workshop! Only 30 spots available! Find all the details here or by clicking the image below!
I hope you are having a wonderful holiday season and feeling the peace and joy of Jesus every day!


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This short article that I read on "Positive Thinking" towards certain things like love, friendship, or certain goals reminds me of a book I read a while back called The Secret. Now, I'm not saying that I agree with the whole books main meaning "think positive thoughts and you'll get what you what." You have to work towards it as well. Maybe they mention that second part in the book, maybe they don't, I'm not quite sure exactly. Anyway, here are a few short sayings you can tell yourself daily, doing these everyday really makes me feel better when I'm having a crappy day.
"I radiate love to those around me and in return they radiate love to me, I do everything in a consciousness of love, I spread love to all those that I meet and they spread love to me, I am surrounded by people who love me and I give thanks for that love, I allow love to find me easily and effortlessly, Love is all around me."


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- Methodology
- Open Access
A modified human ELISPOT assay to detect specific responses to primary tumor cell targets
© Malyguine et al; licensee BioMed Central Ltd. 2004
- Received: 23 December 2003
- Accepted: 29 March 2004
- Published: 29 March 2004
The desired outcome of cancer vaccination is to induce a potent T cell response which can specifically recognize and eliminate autologous tumor cells in vivo. Accordingly, immunological assays that demonstrate recognition of native tumor cells (tumor-specific) may be more clinically relevant than assays that demonstrate recognition of tumor protein or peptide (antigen-specific).
Towards this goal, we adapted the IFN-γ ELISPOT assay to measure immune responses against autologous primary tumor cells in vaccinated cancer patients. As a model system to develop the assay, we utilized peripheral blood mononuclear cells (PBMC) directly isolated from follicular lymphoma patients vaccinated with tumor-derived idiotype protein.
After optimizing several variables, we demonstrated that the modified IFN-γ ELISPOT assay could be used to reliably and reproducibly determine the tumor-reactive T cell frequency in the PBMC of these patients. The precursor frequency of tumor-reactive T cells was significantly higher in the postvaccine PBMC, compared with prevaccine samples in all patients tested. Furthermore, the specificity of these T cells was established by the lack of reactivity against autologous normal B cells.
These results demonstrate the feasibility of quantitating tumor-specific T cell responses when autologous, primary tumor cells are available as targets.
- Peripheral Blood Mononuclear Cell
- Cancer Vaccine
- ELISPOT Assay
- Autologous Tumor Cell
- Primary Tumor Cell
Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials [1–3]. To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined since it would facilitate the rapid comparison of these various formulations.
The clinical efficacy of cancer vaccines is likely to depend on several factors. Firstly, the induced T cells should recognize tumor antigens that are naturally processed and presented in the context of MHC class I and class II molecules on the surface of the tumor. Hence, an immunological assay that demonstrates recognition of native tumor (tumor-specific) may be a more clinically relevant assay to assess T cell responses following cancer vaccination, compared with assays that demonstrate recognition of tumor protein or peptide presented on appropriate antigen-presenting cells (antigen-specific). Secondly, the functional quality and the magnitude of the induced anti-tumor T cell responses are critical for the successful eradication of cancer. Therefore, it is conceivable that an immunological assay that directly measures both the function and frequency of the induced tumor-specific T cells could potentially serve as a surrogate end-point for cancer vaccine trials.
Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated patients but are unable to quantitate individual reactive cells. In contrast, novel assays such as ELISPOT assay, intracellular cytokine assay and tetramer assay can quantitate the frequency of antigen-specific T cells. Of these, the ELISPOT assay is the most sensitive with a general limit of detection of 1 × 105 PBMC when tested with tumor antigens [4]. The assay detects locally secreted cytokine molecules by means of antibody-coated membranes to capture the secreted cytokine derived from the productive interaction of the effector cell and its target cell. The assay has gained increasing popularity, especially as a surrogate measure for cytotoxic T lymphocyte (CTL) responses because it is simple, reliable, sensitive and quantitative [5, 6]. However, the ELISPOT assay has been primarily used for the detection of T cell responses against vaccine components by using peptide or protein pulsed antigen-presenting cells as surrogate T cell targets [7–9]. Demonstration of reactivity to vaccine components does not necessarily equate to recognition and elimination of tumor cells.
Here, we report the development of a modified IFN-γ ELISPOT assay for the direct quantitation of T cell responses against autologous primary tumor cells. To develop the assay, we used follicular lymphoma patients vaccinated with tumor-derived idiotype (Id) protein as a model, since we have previously shown that Id vaccination induces tumor-specific T cell responses in these patients [10].
After obtaining signed informed consent, patients with Stage III or IV follicular center cell lymphoma grade 1 or grade 2 were enrolled on this investigational review board-approved clinical trial. All patients underwent a lymph node biopsy prior to starting treatment to obtain tissue for vaccine production. The lymph node specimen was processed into a single cell suspension and cryopreserved. Patients were initially treated uniformly with a chemotherapy regimen as described previously [10].
Vaccine formulation and administration
Tumor immunoglobulin protein, Id, was isolated from each patient's tumor by heterohybridoma fusion [10]. The appropriate fusions were identified by comparing the Ig VH CDR3 sequences of the fusions with the patient's tumor. The Id was incorporated into liposomes along with recombinant human IL-2 as described previously [11]. Each dose of the vaccine was formulated on a per ml basis with 2 mg of the patient-specific tumor-derived Id protein, 4 × 106 IU of recombinant human IL-2 and 160 mg of dimyristoylphosphatidylcholine lipid (DMPC, Biomira USA Inc., NJ) that was used to generate liposomes. Approximately six months after the completion of the chemotherapy, all patients were administered five doses of the vaccine subcutaneously at months 0, 1, 2, 3 and 5.
Peripheral Blood Mononuclear Cells
Blood samples were obtained from patients at various time points before and after vaccination. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient separation with Ficoll Isopaque (ICN Biomedicals Inc., Aurora, OH) and cryopreserved for immunological assays. Pre- and postvaccine PBMC were thawed, washed and resuspended to a concentration of 1–3 × 106 cells/ml in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA), supplemented with 5% FBS (Hyclone, Logan, Utah), 1 mM sodium pyruvate (BioWhittaker, Walkersville, MD), 20 mM HEPES buffer (Invitrogen), 50 μM β-mercaptoethanol (Sigma, St. Louis, MO), 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen) (complete medium). Five ml of the cell suspensions were plated into each well of 6 well plates (Corning, Inc. Corning, NY) and rested overnight at 37°C, 5% CO2. The next day, PBMC were harvested, washed and cell viability was assessed by trypan blue prior to use in the ELISPOT assay.
Activation of tumor cells and normal B cells
Cryopreserved cells from the lymph node biopsy specimen were enriched for tumor cells by depletion of T cells with CD3 microbeads over a magnetic column (Miltenyi Biotec, Auburn, CA) using the manufacturer's protocol. Autologous normal B cells were isolated from PBMC by magnetic cell separation method using the B Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer's protocol. The purity of the isolated tumor and normal B cells was >95%. Tumor cells and normal B cells were activated for 3 days with 800 ng/ml of recombinant human soluble CD40 ligand trimer (sCD40Lt, Amgen, Thousand Oaks, CA) and recombinant human IL-4 (2 ng/ml) (Peprotech, Rocky Hill, NJ). Activated tumor cells and normal B cells were harvested and washed prior to co-culture with PBMC in the ELISPOT assay.
IFN-γ Enzyme-linked immunospot (ELISPOT) assay
MultiScreen-IP opaque 96-well plates (High Protein Binding Immobilon-P membrane, Millipore, Bedford, MA) were coated overnight at room temperature with 50 μl/well of 20 μg/ml mouse anti-human IFN-γ mAb (BioSource, Camarillo, CA) in DPBS (Invitrogen). After overnight incubation, the plates were washed three times with 200 μl DPBS/well and blocked with 200 μl/well of RPMI 1640, 10% human AB serum (Mediatech, Herndon, VA), 25 mM Hepes, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen) for 2 h at 37°C, 5% CO2. Harvested effectors were added to the plates in triplicates at 1 × 105 cells/well with either culture medium alone or with 2 × 105 cells/well sCD40Lt activated autologous tumor cells and cultured for 48 h at 37°C, 5% CO2. The plates were washed manually 6 times with 200 μl/well of DPBS/0.05% Tween. 50 μl/well of 2 μg/ml mouse anti-human IFN-γ-biotinylated mAb (BD Pharmingen, San Diego, Ca) in DPBS/1%BSA/0.05% Tween was added and the plates were incubated for 2 h at room temperature. Plates were manually washed 4 times with DPBS and 50 μl/well of streptavidin HRP (BD Pharmingen) diluted 1:2000 in DPBS/1% BSA was added for 1 h at room temperature. The plates were washed a final 4 times with DPBS. Spots were visualized by adding 100 μl/well of TrueBlue™ Peroxidase Substrate (KPL, Gaithersburg, MD) for 2 min. Plates were scanned and counted using the ImmunoSpot analyzer (Cellular Technology, Ltd., Cleveland, OH) to determine the number of spots/well. The precursor frequency of tumor-specific T cells was determined by subtracting the background spots in tumor alone and PBMC alone from the number of spots seen in response to tumor cells.
Statistical analysis
Statistical analysis was performed using Student's t-test for paired mean values and Pearson correlation coefficient (R2).
Optimization of tumor IFN-γ ELISPOT assay
Summary of assay conditions that were tested and selected for the autologous tumor IFN-γ ELISPOT assay
Item optimized
Selected for assay
Clear vs. Opaque
Targets (tumor cells)
sCD40Lt stimulated vs. non-stimulated
sCD40Lt stimulated
Effectors (PBMC)
Overnight cultured vs 2 h cultured
Overnight cultured cells
Effector:Tumor Ratio
100:200K cells/well
Incubation time of assay
24 and 48 hours
48 hour
5% and 10% HuAB vs. 5% and 10% FBS
10% HuAB
Substrate Detection System
AEC, TrueBlue™, and BCIP
Opaque plates were optimal for analysis of IFN-γ spots
Opaque and clear plastic versions of MultiScreen-IP ELISPOT plates were compared to facilitate optimal spot analysis using the ImmunoSpot analyzing system. The opaque version was most advantageous for ELISPOT enumeration. The opaque plastic precluded light-refraction from the reader's light source and therefore eliminated alterations in background color of individual wells that interfered with the ability to correctly enumerate spots. The ImmunoSpot analyzing system was able to auto-center opaque, but not the clear plastic plates, thereby facilitated automated analysis.
sCD40Lt activated tumor cells enhanced cytokine production by T cells
Activation of B cell tumor cells with sCD40Lt upregulated various costimulatory molecules and MHC class I and class II molecules on the surface of tumor cells associated with enhanced antigen presenting capability [[12] and Neelapu et al., manuscript submitted]. Our pilot experiments indicated that activation of tumor cells with sCD40Lt markedly enhanced the sensitivity of the ELISPOT assay by increasing the IFN-γ production by responding T cells as compared to unmodified tumor cells (data not shown). We have therefore used sCD40Lt activated tumor cells as stimulators to evaluate T cell responses in this assay.
Resting PBMC overnight improved the reactivity of effector cells
Effector to tumor cell ratio
Longer assay incubation time detected higher number of tumor-reactive T cells
10% Human AB serum was optimal for culture medium
Substrate detection system
IFN-γ spots produced by pre- and postvaccine PBMC using the optimized assay
Based on preliminary testing, the protocol described in the materials and methods was established as optimal (Table 1). Triplicate wells demonstrating the IFN-γ spots produced by pre- and postvaccine PBMC analyzed in parallel from a representative patient are shown in Figure 6. A significantly higher number of IFN-γ spots were detected in the postvaccine PBMC as compared to the prevaccine sample (p < 0.001).
Reproducibility of the tumor IFN-γ ELISPOT assay
Specificity of the tumor-reactive T cell responses
Our data demonstrate that the ELISPOT assay could be easily adapted to reliably and reproducibly determine the precursor frequency of tumor-specific T cells in follicular lymphoma patients immunized with an autologous tumor-derived Id vaccine. We have also been able to use a modification (5 day tumor activation, 72 h incubation) of this assay to quantitate the tumor-specific T cell responses in mantle cell lymphoma patients vaccinated with Id (data not shown). Thus, this assay fulfilled our stated objectives for a clinically relevant immunological assay by demonstrating that it could functionally (IFN-γ production) and quantitatively assess the T cell responses induced by a cancer vaccine against autologous primary tumor cells.
As opposed to the traditional antigen-specific assays that assess immune responses against vaccine components (peptides or proteins), tumor-specific immunological assays in addition to being more clinically relevant can have several advantages. Firstly, tumor-specific assays can potentially detect both CD4+ and CD8+ T cell responses since endogenous antigens are presented by both MHC class I molecules as well as MHC class II molecules [[13–15] and Neelapu et al., manuscript submitted]. In contrast, antigen-specific (protein) assays detect mostly CD4+ T cell responses since the soluble exogenous antigen is predominantly processed in the endosomal pathway and presented on MHC class II molecules. Secondly, tumor-specific assays can be used in patients with any HLA phenotype unlike peptide assays that are usually restricted to a single HLA phenotype (e.g. HLA-A*0201) depending on the binding affinity of the peptide. Thirdly, tumor-specific assays allow monitoring of patients when whole or lysed tumor cells are used as the immunogen and the tumor-specific antigens have not been defined. Finally, tumor recognition assays potentially enable the detection of immune responses against the immunogen as well as other antigens not represented in the vaccine. For instance, they can detect immune responses against cryptic epitopes that may develop by epitope spreading, secondary to the inflammatory response initiated by T cells specific against the immunogen [16, 17].
A probable limitation for tumor recognition assays is the availability of tumor cells. While primary tumor cells are easily accessible for some cancers (e.g. lymphoma, leukemia, myeloma, melanoma), they may not be generally accessible for certain other cancers (e.g. breast cancer, renal cell cancer). When available, it may be feasible to adapt the IFN-γ ELISPOT assay for the quantitative assessment of T cell responses against primary tumor cells as a preferable alternative to antigen-specific assays. Such quantitative tumor-specific immunological assays need to be eventually evaluated in large clinical trials to determine their validity as surrogate end-points for clinical efficacy of cancer vaccines.
We adapted the IFN-γ ELISPOT assay to directly measure immune responses against autologous primary tumor cells in vaccinated cancer patients. We demonstrated that the modified IFN-γ ELISPOT assay could be used to reliably and reproducibly determine the tumor-reactive T cell frequency in the PBMC of these patients when sCD40Lt activated tumor cells were used as targets. The precursor frequency of tumor-reactive T cells was significantly higher in the postvaccine PBMC, compared with prevaccine samples in all patients tested. Furthermore, the specificity of these T cells was established by the lack of reactivity against autologous normal B cells. These results demonstrate the feasibility of quantitating tumor-specific T cell responses when autologous, primary tumor cells are available as targets.
We thank Amgen for generously providing the sCD40Lt and Biomira USA Inc., for manufacturing the vaccine. Contract NO1-CO-12400 Funded by the National Cancer Institute.
The Content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, not does mention of trade names, commercial products, or organisations imply endorsement by the U.S Government.
Authors' Affiliations
- Rosenberg S: Progress in human tumor immunology and immunotherapy. Nature. 2001, 411: 380-384. 10.1038/35077246.View ArticlePubMedGoogle Scholar
- Wang R, Rosenberg S: Human tumor antigens for cancer vaccine development. Immunol Rev. 1999, 170: 85-100.View ArticlePubMedGoogle Scholar
- Kwak L: Translational development of active immunotherapy for hematologic malignancies. Semin Oncol. 2003, 30 (3 Suppl 8): 17-22. 10.1016/S0093-7754(03)00232-X.View ArticlePubMedGoogle Scholar
- Keilholz U, Weber J, Finke J, Gabrilovich D, Kast W, Disis M, Kirkwood J, Scheibenbogen C, Schlom J, Maino V, Lyerly H, Lee P, Storkus W, Marincola F, Worobec A, Atkins M: Immunologic monitoring of cancer vaccine therapy: results of a workshop sponsored by the Society for Biological Therapy. J Immunother. 2002, 25: 97-138. 10.1097/00002371-200203000-00001.View ArticlePubMedGoogle Scholar
- Whiteside T: Immunologic monitoring of clinical trials in patients with cancer: technology versus common sense. Immunol Invest. 2000, 29: 149-162.View ArticlePubMedGoogle Scholar
- Schmittel A, Keilholz U, Thiel E, Scheibenbogen C: Quantification of tumor-specific T lymphocytes with the ELISPOT assay. J Immunother. 2000, 23: 289-295. 10.1097/00002371-200005000-00001.View ArticlePubMedGoogle Scholar
- Peterson A, Harlin H, Gajewski T: Immunization with Melan-A peptide-pulsed peripheral blood mononuclear cells plus recombinant human interleukin-12 induces clinical activity and T-cell responses in advanced melanoma. J Clin Oncol. 2003, 21: 2342-2348. 10.1200/JCO.2003.12.144.View ArticlePubMedGoogle Scholar
- Asai T, Storkus W, Mueller-Berghaus J, Knapp W, DeLeo A, Chikamatsu K, Whiteside T: In vitro generated cytolytic T lymphocytes reactive against head and neck cancer recognize multiple epitopes presented by HLA-A2, including peptides derived from the p53 and MDM-2 proteins. Cancer Immun. 2002, 2: 3-PubMedGoogle Scholar
- Meidenbauer N, Harris D, Spitler L, Whiteside T: Generation of PSA-reactive effector cells after vaccination with a PSA-based vaccine in patients with prostate cancer. Prostate. 2000, 43: 88-100. 10.1002/(SICI)1097-0045(20000501)43:2<88::AID-PROS3>3.0.CO;2-G.View ArticlePubMedGoogle Scholar
- Bendandi M, Gocke C, Kobrin C, Benko F, Sternas L, Pennington R, Watson T, Reynolds C, Gause B, Duffy P, Jaffe E, Creekmore S, Longo D, Kwak L: Complete molecular remissions induced by patient-specific vaccination plus granulocyte-monocyte colony-stimulating factor against lymphoma. Nat Med. 1999, 5: 1171-1177. 10.1038/13928.View ArticlePubMedGoogle Scholar
- Kwak L, Pennington R, Boni L, Ochoa A, Robb R, Popescu M: Cutting Edge: Liposomal formulation of a self lymphoma antigen induces potent protective anti-tumor immunity. J Immunol. 1998, 160: 3637-3461.PubMedGoogle Scholar
- Schultze J, Cardoso A, Freeman G, Seamon M, Daley J, Pinkus G, Gribben J, Nadler L: Follicular lymphomas can be induced to present alloantigen efficiently: a conceptual model to improve their tumor immunogenicity. Proc Natl Acad Sci USA. 1995, 92: 8200-8204.PubMed CentralView ArticlePubMedGoogle Scholar
- Nuchtern J, Biddison W, Klausner R: Class II MHC molecules can use the endogenous pathway of antigen presentation. Nature. 1990, 343: 74-76. 10.1038/343074a0.View ArticlePubMedGoogle Scholar
- Sant A: Endogenous antigen presentation by MHC class II molecules. Immunol Res. 1994, 13: 253-267.View ArticlePubMedGoogle Scholar
- Maecker H, Dunn H, Suni M, Khatamzas E, Pitcher C, Bunde T, Persaud N, Trigona W, Fu T, Sinclair E, Bredt B, McCune J, Maino V, Kern F, Picker L: Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods. 2001, 255: 27-40. 10.1016/S0022-1759(01)00416-1.View ArticlePubMedGoogle Scholar
- Lally K, Mocellin S, Ohnmacht G, Nielsen M, Bettinotti M, Panelli M, Monsurro V, Marincola F: Unmasking cryptic epitopes after loss of immunodominant tumor antigen expression through epitope spreading. Int J Cancer. 2001, 93: 841-847. 10.1002/ijc.1420.View ArticlePubMedGoogle Scholar
- Disis M, Gooley T, Rinn K, Davis D, Piepkorn M, Cheever M, Knutson K, Schiffman K: Generation of T-cell immunity to the HER-2/neu protein after active immunization with HER-2/neu peptide-based vaccines. J Clin Oncol. 2002, 20: 2624-2632. 10.1200/JCO.2002.06.171.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.


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Charlesbridge Children's Books Blog — German Book Office
Top 10 Surprises from a Yankee Editor in Germany
Posted by Cindy Ritter on
By Julie Bliven In late June I traveled to Germany with seven other children's book editors hailing from the US, UK, New Zealand, and Canada. Beginning in Hamburg and ending in Frankfurt, we met with about a dozen publishers to talk shop and learn about the German book market. The trip was sponsored by the German Book Office of New York, which is co-financed by the German Foreign Office and is one of the Frankfurt Book Fair's arms into foreign markets. The GBO encourages interest in German books by promoting rights and license sales and book exports through...


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|Posted by Sean on January 20, 2014 at 11:20 PM
ACS Oregon now has a public relations officer!
We sent a representative to the ACS public relations workshop early this summer in order to learn some of the tricks of the trade and better promote Oregon local section chemists and chemistry.
The number one take home message….the best stories are about people not science!
We know we have some pretty interesting people in the Oregon local section, so if you have a story we'd love to hear about it.
Categories: Informational
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The words you entered did not match the given text. Please try again.


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To be sure by the product made from a Neoprene, the contact surface with an Air-cleaner VOX has stuck well.However, since elasticity of the Neoprene was bad, pains was taken over attachment. Compared...
Since the Filter was like tattering powder when overhauling the Air-filter VOX, when it searched confusedly, the splendid price used that it was unexpected.Since DT-1 was cheap No. 1 in it, it thought...
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Nine Negotiation Tips to Get the Best Deal
When it comes to buying a home, your negotiation knowledge can go a long way to protecting your interests and helping you get the home you want. Keep these tips in mind as you proceed through the buying process and remember you will get the best advice and outcome if you allow your agent to become a buyers' agent. Ask how.
Knowing what is really important to you early on will go a long way to making your experience smoother and faster. Ultimately, the bar for whether your negotiation is successful is determined by what the home and terms are worth to you.
2. Know your local environment
The best negotiators are the most informed. Ask your agent to provide data on recent sold prices for similar homes, average difference between asking and sold price, and average days on the market for similar properties.
3. Get the inside scoop
Don't make a blind offer. Ask your agent to explain market data to you and how that might relate to your transaction. Your buyers' agent may also know some valuable transaction know some valuable transaction politics like whether or not the seller may need to sell and move quickly.
4. Problem solve for all parties
Traditionally, negotiations were a two way power struggle between buyer and seller. Now, however, buyers do well to consider all parties that may be involved in the transaction - seller, lenders, appraisers, and maybe even homeowners associations. It is more important than ever to approach your negotiation with a win-win attitude with the aim of meeting as many needs as possible.
5. Bond with your lender
Too often transactions fall through at the financing stage. Work in advance with your mortgage lender to make sure you provide all necessary documents and possibly even secure back-up financing if things go wrong. Not waiting until it happens will prevent a great deal of angst.
6. Manage your own mindset
Remember during the process that the least emotional parties have the most success. The more attached you are to a particular home or price point, the more likely you are to panic or cave in on important points. Lean on and listen to your agent for objective support throughout the transaction. It's their job to make sure you a making the most informed and positive decisions.
7. Learn and accept the "negotiables"
Learn early what is and what isn't within each party's control. Your agent can help you with this and you can avoid the emotional exhaustion that comes from haggling in areas that aren't negotiable like the bank's bottom line or cosmetic repairs.
8. Minimize time pressures
When buying or selling, changing or moving deadlines can cost you thousands of dollar and perhaps even terminate the offer. To prevent problems plan and search early when possible, locate temporary rentals, and develop an alternate plan if your closing is delayed or you encounter last minute hurdles.
9. Act quickly - not impulsively


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Guardian writers' prediction 15th (This is not Stuart James's prediction, but the average of our writers' tips)
Last season's position 17th
Odds against winning the title 2,500-1
It is a measure of how far Wolverhampton Wanderers have come under Mick McCarthy that this will be the first time since 1982 that they have spent three successive seasons in the top flight. One thing is for sure, the Wolves manager is not the sort of person who will take a fourth Premier League campaign for granted. In fact, there is more chance of him signing up to Twitter, the social networking site that he despises and on which he half-expects a "disgruntled numpty" to reveal his starting line-up at some stage this season.
The reality, though, is Wolves should be much better equipped than the three promoted clubs and also capable of competing on a level footing with the likes of Newcastle United, West Bromwich Albion, Blackburn Rovers, Wigan Athletic and Bolton Wanderers, none of whom are expected to be tearing up trees in the Premier League this season.
McCarthy likes to get his transfer business done early, and this summer spent the best part of £11m on Jamie O'Hara and Roger Johnson. Perhaps just as importantly, he has held on to his better players. Matt Jarvis, who won his first England cap in March, and Kevin Doyle, who seems to attract speculation every summer, both come into that category.
Steven Fletcher might also feel that he deserves to be mentioned in the same breath after finishing last season with a flourish, scoring six goals in an eight-match spell that was crucial in lifting Wolves to the 40-point mark that secured their Premier League status. With 10 Premier League goals to his name, Fletcher scored twice as many as Doyle from 10 fewer starts – statistics that suggest he must spend more time this season playing alongside the Republic of Ireland international in a conventional strike partnership (something that happened rarely last term).
Clean sheets were also scarce, making it no surprise that McCarthy moved for Johnson. The centre of the Wolves defence has needed addressing for some time. Michael Mancienne was a liability because of his lack of concentration (Hamburg supporters, prepare to be disappointed), Richard Stearman never seems comfortable at this level and Jody Craddock turned 36 last month, all of which leaves Christophe Berra as Johnson's likely partner in the middle.
This is a big season for Johnson. He has been appointed as the new Wolves captain, in place of Karl Henry, and has recently spoken about his ambitions of breaking into the full England set-up, after being named in the last four provisional squads. He can be a commanding central defender but he will need to play a lot better than he did for Birmingham City in the final few months of last season, when his form suffered without Scott Dann alongside him.
O'Hara, in contrast, has already made his presence felt at Wolves, having enjoyed a successful loan spell last season. Comfortable on the ball and with an eye for goal, he should be a valuable addition. He looked particularly bright when playing in behind Doyle, although most Wolves fans will hope to see him in a slightly deeper role, if only because it will mean that McCarthy is setting his team up in a 4-4-2 formation rather than 4-5-1.
If there are any more arrivals before the transfer window closes, it seems likely McCarthy will target the full-back positions and central midfield. He has options in other areas, including on the flanks, where Stephen Hunt will be hoping he can start this season in the same way that he finished the last. If only the luckless Michael Kightly, who has made two starts in the last 22 months, could stay fit and get back to playing the way he was when Wolves won promotion from the Championship in 2009.
Since arriving in the Premier League, Wolves have been a model of inconsistency, underachieving one minute and overachieving the next, leaving their supporters scratching their heads. In 2009-10 they took six points off a Tottenham Hotspur side that finished fourth. Last season, they beat Manchester United, Chelsea and Manchester City, the top three, at home and won at Anfield. In total they picked up 13 points against the top six.
If Wolves proved anything during that campaign it was that you write them off at your peril. So many of their victories were either against the odds or on the back of poor performances, when the pressure was on, which says much for the character in the squad and the belief McCarthy has instilled in his players. The other side of the coin, however, is that they collected only two wins and two draws against the other six clubs in the bottom seven. That needs to change this season because it is difficult to believe they can repeat their superb results against the top teams.
Some off-the-field results have been just as impressive. Wolves made a £9m pre-tax profit for the year to 31 May 2010 – they were one of only four Premier League clubs not in the red – and they have no debt. Their wage bill is only 49% of turnover and their accounts also showed £25m in the bank. It is little wonder that Steve Morgan, the club's owner, believes that Wolves provide a template for other clubs to follow.
Indeed, with Molineux currently undergoing the first phase of an extensive rebuilding programme that will increase the capacity and improve facilities, these are exciting times for Wolves, who are laying the foundations on and off the pitch to become an established Premier League club. McCarthy knows, however, that they are not quite there yet.


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All Films.All Above Board.NOW INCLUDES TV SHOWS & TV LISTINGS
The true life story of a mother and daughter duo who became the most successful female country music artists in the United States of America. Kathleen York plays Naomi Judd, whose daughter, Wynonna (Viveka Davis), joins her in forming The Judds. The mother and daughter combination go on to sell a large number of records and even obtain Granny nominations, but will the success they enjoy alter the nature of their relationship?


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Massage Adelaide Reflections - Adelaide's Best Beauty Salon and Day Spa Retreat
Welcome to Massage Adelaide!
That means you can simply lie there and collapse while we massage away your troubles at least for an hour. We use warm aromatic essential oils of sandalwood, lavender, orange and patchouli to soften your skin and calm your senses. Your skin will feel velvety soft and smooth and your muscles will feel relaxed.
All of our treatment rooms are temperature controlled so they can be nice and toasty or chillout cool - depending on the weather! The treatment rooms are all private and we use clean fluffy towels for each guest - no need to worry about hygiene, we never reuse the same towel! We use professional grade massage oils so you can relax and enjoy your massage with us.
Our day spa is located in Adelaide, South Australia, are tastefully decorated and we cater for men and women, it's a great gift for that hard to buy for man or woman in your life!
Aroma Massage Adelaide
Our aroma massage is a relaxing style of massage that relieves stress and tension, with our bevy of flowing massage moves, that will surely turn you into massage mush. Our aroma massage is a must for stressed out, over-worked and tired people who want a little luxury time out for themselves, and we know you will enjoy it! Using a selection of mellowing essential oils to reduce stress, calm the nerves and fight off fatigue and exhaustion.
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With this massage we focus on spots that are bothering you, whether it is from a tender burning shoulder from too much computer work, lousy lower back, stiff neck or troubled, tired legs and feet. Warm paraffin wax is also placed on your 'sore-spots' helping to reduce inflammation, soothe tender-knots and loosen you up and relax your muscles – a must for office-dwellers when you get that burning feeling.
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Massage Adelaide


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Washington, D.C.
Washington, D.C.
The judicial system does not contact people telephonically and ask for personal information such as your Social Security number, date of birth or credit card numbers. If you receive one of these phone calls, do not provide any personal or confidential information to these individuals. This is an attempt to steal or to use your identity by obtaining your name, Social Security number and potentially to apply for credit or credit cards or other loans in your name. It is an attempt to defraud you.
If you have already been contacted and have already given out your personal information, please monitor your account statements and credit reports, and contact your local FBI office. Local FBI field office telephone numbers can be found in the front of your local telephone directory or on www.fbi.gov. For further information, please review the warnings posted on the U.S. Courts website at www.uscourts.gov, "Newsroom" news article "WARNING: Bogus Phone Calls on Jury Service May lead to Fraud," August 19, 2005.


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I'm soooo in love with these shorts!
I love that they can be easily "upgraded" to a more classy and dressy look with a pair of a heels or wedges and a blazer! (Pictures of this look coming up)
Last Saturday, I opted for a casual easy look with a pair of sandals and a simple black top from H&M.
Another thing I love about these shorts is that they're high waist and therefore very figure flattering!
Aren't they just gorgeous?!


text: From: Shawn Steele <email@example.com> Message-Id: <email@example.com> Date: Wed, 15 May 1996 17:03:44 -0600 In-Reply-To: email@example.com To: firstname.lastname@example.com Subject: My vote I'd like to also include my vote for the <BANNER> tag. It's simpler than using frames and it would save me some space on my pages. (Most web pages out there have a banner-like bar of links on top & some have them on the bottom too for those users who scroll to the bottom but are too lazy to go back to the top to pick another link. Of course as per my previous message I'd like to see some sort of client <INCLUDE> tag to work with this as well to save even more bandwidth !:) - shawn


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Industrial data firm Drilling Info Inc. has provided data to Reuters showing a decline of around 40% in new US oil and gas applications in the month of November.
In October there were 7,227 recorded applications; however a month later only 4,520 applications were received across the US.
This represents one of the largest declines in applications since the shale boom began, the CEO of Drilling Info, Allen Gilmer, suggested that the sudden change was a "very quick response" to further plunges in oil prices. US crude prices settled $66.88 by end of trading on Tuesday.
Read more at: Decline of nearly 40% in new US oil and gas applications for November


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Thursday, May 25, 2017
Li Shufu, the man who dared to buy Proton
After years of dwindling sales and financial losses, it is hoped that finally now, Shufu is the man who would work his "magic"' in reviving Proton as he did with prestigious Swedish auto maker Volvo.
Given his proven track record in turning round loss-making auto companies, the Malaysian government and Proton's parent company DRB-Hicom are confident he will herald a new era of growth for Proton.
Yesterday, DRB-Hicom signed an agreement with China-based Zhejiang Geely Holdings Group Ltd for the Chinese car group to acquire 49.9% in the national car maker.
Under the deal, Proton would also dispose its entire stake in British sports carmaker Lotus for 100 million pounds (RM1=RM5.57), a move that will enable Proton to cut its losses.
As Geely's owner, Shufu owns 100% of Volvo, yet many might not be aware that the owner hails from China.
Shufu is rare among today's many Chinese entrepreneurs for taking on auto manufacturing as it is a sector dominated by state enterprises and multinationals.
He founded Geely in 1986 to build refrigerators and became an automaker in 1997 when he wanted to produce a cheap car for the masses.
Geely Group now has reached 1.3 million car sales in the financial year 2016.
And, Shufu is now reportedly worth a staggering US$7bil.
As an entrepreneur-owner, his almost 50% ownership is a major plus point which Proton would derive from the partnership.
This is because Geely is a "entrepreneur driven" auto company and one of the few left in the world as opposed to car firms owned by state enterprises and multinationals.
As an entrepreneur-owner, there would undoubtedly be a greater sense of personal commitment to ensure that Proton achieves success at the domestic, regional and possibly global levels.
No wonder he is sometimes referred to as the ''Henry Ford of China.'' Before he bought Volvo in 2010, the Swedish car maker owned by Ford then was on the brink of extinction.
He also owns the iconic London taxi Company.
With Geely's expertise, one can expect new Proton models, transfer of technology, more jobs, auto engineers and bigger orders for parts vendors.
The partnership can also help ramp up production capacity of its under-utilised Tanjung Malim plant to its full annual capacity.
The Chinese firm''s acquisition of Proton nicely fits into its plans to make its presence felt in South-east Asia for which it has been on the lookout for a manufacturing plant in the region.
Investor sentiment on Geely has been immensely favourable with its share price on the Hong Kong Stock Exchange having risen from HK$3.77 in 2014 to HK$11.50 as of May 22, 2017.
Only time will tell whether Proton would emerge as a formidable automative player again.
Nevertheless, with Shufu and Geely providing solid backing, our very own and much-loved Proton brand now has a real chance of making a comeback, and probably a huge comeback at that- Bernama
Posted by wikisabah at 5:47:00 PM
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