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I'm so excited to be able to talk about my new pocket-size book on green cleaning! After numerous edits and an incredibly patient and talented designer, it's finally at the printer doing its first run in print format. For those of you who purchased my Spring Cleaning E-book in the past, this is a much more extensive guide that goes beyond ... Read More »
Buying Halal
Green Deen Book Giveaway!
If you haven't heard of Green Deen: What Islam Teaches About Protecting the Planet or the book's author, Ibrahim Abdul-Matin, you may want to pay close attention. He's one of the first Muslims in recent history to call people to live a more eco-friendly existence, all within the practical and sensible teachings of Islam. His book also serves as an incredibly great teaching tool ... Read More »
Free Subscription to Halal Consumer Magazine
Ever wonder what or who is involved in the halal-certification process for foods, vitamins and other consumer products? Want to know the latest products that are being made and stamped with a halal seal of approval? IFANCA (Islamic Food and Nutrition Council of America) is in the business of doing just that, just for you, the halal consumer. Headquartered in Chicago, ... Read More »
Brown Basmati Rice with Ghee & Saffron
I love the smell of basmati rice. There isn't any other rice quite like it. When you walk into the home of someone cooking it, you can usually look forward to something interesting, a one-pot dish paired with meat, like biryani made with chicken, dried plums, exotic spices and of course, fragrant basmati rice. Now available at stores like Trader Joe's is ... Read More »
Food Styling for a Halal Ad Campaign by IFANCA
I can't tell you all how excited I am to finally be able to talk about this- my first time professionally styling food for a TV commercial! It was many months ago that I prepped the food and made it look pretty for the camera, but our lips were sealed until IFANCA, the Islamic Food & Nutrition Council of America, ... Read More »
New! Colored Aprons Make the Perfect Holiday Gift for Your Favorite Cook (or Yourself)
I'm so excited to announce the arrival of our new apron colors. We still have the traditional Creme Fraiche White aprons, but have added a few more fun colors to the mix. My Halal Kitchen aprons now come in the following colors: Blueberry Blue, Basil Green, Strawberry Red and Coffee Brown! Perfect for those who cook with foods and spices that are ... Read More »
Chocolate Mousse Cups | 'Eid Bazaar Baking
I had the great fortune of being able to do a little baking for a local school bazaar this past weekend. Teachers were raising money to fund the children's Hajj re-enactment program where the kids learn how to properly perform the Hajj, or pilgrimage to Mecca. You see, it's Hajj 'season', meaning that most piligrims from all over the world ... Read More »
Meet Chief Halal Officer Adnan Durrani and Saffron Road Halal Frozen Entrees
It wasn't too long ago that I first heard about Saffron Road, halal frozen entrees that are now sold at Whole Foods stores. At first glance, I thought they were just another frozen ethnic entree that would be out of reach for halal-eating Muslims. Upon closer look at the packaging, I noticed the words Halal Cuisine. Digging a bit further, I ... Read More »
Culinary Delights at Northeast Ohio Farmers' Markets
This year during my stay in Ohio I tried to visit as many farmers' markets and produce stands as possible so that I could get a grasp on the local food buzz- who's who in local farming and vending and what's the trend for organic and sustainable products. The Tremont Farmers' Market & the tower at Cleveland's West Side Market located ... Read More »
Halal in Cleveland
For a city not ranked as one of the largest in the U.S., Cleveland has a lot to offer in the way of halal foods. There's an area on Cleveland's west side that is home to a large Arab-American population, many of whom are of Palestinian descent and Muslims who seek out halal foods. Within just a few blocks of ... Read More »


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Share your comments
Abraham Lincoln : vampire hunter
Seth Grahame-Smith
Adult Fiction GRAHAME
Seth Grahame-Smith
Adult Fiction GRAHAME
What other readers are saying about this title:
KaliO said:
Best known for living in a log cabin as a boy and ending slavery as our illustrious sixteenth president, a diary by the man himself (fortuitously discovered by Seth Grahame-Smith, author of Pride and Prejudice and Zombies) reveals that Honest Abe was also a skilled slayer of vampires. Following his mother's death at the hand (or bite) of a blood-sucking creature of the undead, young Abraham vows to spend the rest of his life ridding this great nation of the foul demon presence. And since slavery is a projection of the vampires' natural desire for control over their victims, Abe vows to defeat that vile institution as well. His legendary strength and height are a definite advantage; his practiced skill with his sharp ax serves him well as he fights to crush the vampires' political power—and just plain chop their heads off. The road to victory (and the White House) is not easy, and Abraham faces an uphill battle fraught with failed love affairs, sickly sons, dying soldiers, disguised vampires, and bloody fangs. Complete with documentary photographs, diary entries, quotes from letters, and explanatory footnotes, Abraham Lincoln, Vampire Hunter has the look and feel of a grand historical biography—but with tongue firmly in cheek. Continuing his tradition of adding scenes of gory mayhem to solid classics, Grahame-Smith might cause history buffs to grumble, but horror and humor fans will be tickled pink by the image of Honest Abe swinging his trusty ax at hoards of blood-thirsty sharp-toothed fangs.
posted Apr 3, 2010 at 11:59AM
Marsap said:
A little bit of history (sort of) and a lot of gory sci-fi. It was a fun read--great summer read.
posted Jul 7, 2011 at 10:35AM
Wolfzues said:
This book is an amazing read...little history lession and a fun book if you like vampire stories. This book is also great in audio book format.
posted Dec 17, 2011 at 9:38AM
Add a Comment
|Submission Guidelines
Find this title in the Library Catalog
Abraham Lincoln
Mother and grandfather were killed by vampires; determined to rid the nation of vampires.
President of the United States

subset: commoncrawl


text:
My normal links are not working for this. I think it started at 11am. Any help with a link is appreciated.
Originally Posted by chadm
My normal links are not working for this. I think it started at 11am. Any help with a link is appreciated.
Having the same issue...
Yeah, the listen live isn't really working. Meaning, it says it's playing... but there's no audio. I'm assuming it's a technical error and it'll be up shortly.
Williams!!! Going off on Arnaud haters!!! Loveit! Show sounds great today guys!
"I think that people are responding to someone standing up and saying this is what we want to do and let's not be afraid to go for it,'' Pollard said. "I think Iowa State has a very conservative, modest background. We never pound our chest and say `Let's do it.' It's OK to set a goal. You can't do it if you don't say what you want to do. You should be proud of it.''
Jamie Pollard, 6/2/2006
Forum Rules
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Archived:Listing calendar entries in Flash Lite
Archived: This article is archived because it is not considered relevant for third-party developers creating commercial solutions today. If you think this article is still relevant, let us know by adding the template {{ReviewForRemovalFromArchive|user=~~~~|write your reason here}}.
We do not recommend Flash Lite development on current Nokia devices, and all Flash Lite articles on this wiki have been archived. Flash Lite has been removed from all Nokia Asha and recent Series 40 devices and has limited support on Symbian. Specific information for Nokia Belle is available in Flash Lite on Nokia Browser for Symbian. Specific information for OLD Series 40 and Symbian devices is available in the Flash Lite Developers Library.
This code snippet shows how to list all calendar entries of the default calendar using the Calendar Service API in Flash Lite 3.0 (supported from S60 5th Edition onwards).
S60 5th Edition
// Import Platform Service Interface
import com.nokia.lib.Service;
// Heading of the application
heading_txt.text = "List Calendar entries";
// Create a new Service object which has Calendar data
var calender = new Service("Service.Calendar", "IDataSource");
// Define input parameters
var inParams = {Type:"CalendarEntry"};
// Define result value
var outParams = calender.GetList(inParams);
if (outParams.ErrorCode == 0) {
var outList = outParams.ReturnValue;
var outputEntry = null;
// Go through all calendar events and print them to the textbox on the
// scene
do {
outputEntry = outList.next();
if (null != outputEntry) {
var id = outputEntry.id;
text_txt.text += "-"+id+"\r";
} else {
} while (true);
} else {
var errorId = outParams.ErrorCode;
text_txt.text += "Error: "+errorId;
IDs of all the calendar items of the default calendar are displayed.
Example application
The following sample application has been tested in Nokia 5800 XpressMusic (S60 5th edition, Flash Lite 3.0).
File:FlashLite List Calendar Entries.zip


text:
Story behind the photo: Men at Work
It was somewhere near Checkpoint Charlie, the most famous crossing point between East- and West-Berlin during the Cold war, that I spotted this setting.
Even though the Berlin wall has been pulled down for over twenty years by now and the checkpoint is nothing more than another tick on the to do-list of every Berlin visitor, I found that this image resembles the general German post-war idea.
Things needed to be rebuilt.
Germans are not the kind of people who sit in a corner and whine about what happened. They get together, take their tools and get the job done. I am amazed how they can have so much trust in the future after having part in three major wars in less than a hundred years.
The second aspect why this photo - which, when you look at it, could have been taken everywhere - felt so right in Berlin, is because of the structure of the setting. Everything looks straight, neat and very well coordinated, very German stereotype.
Everything is were it's supposed to be.
Things get done.
Quickly and organized.
If you like this post and you like to hear more of what I have to say, keep in touch by registering to my RSS feed and be the first to read new posts.
Posts you may also like:
Photo Essay: East Side Gallery (or the Berlin Wall anno 2011)
Story behind the photo: Budapest Chain Bridge
Albertarocks' Reviews Webpage 96Canada's also been infiltrated but for some motive, Canada won't however seem to be significantly of the target on the sights in the NWO gang. We've got our demons the following very. To illustrate, they maintain aiming to generate command to censor the world wide web (slowly but surely and insidiously), plus the most people keep kicking them inside of the balls for their initiatives. But in the end, they are going to acquire that fight also if we do not stop them by some means.Oct 31 10:28 AMHi John. The sort of job and examination I do includes, but is without doubt not modest to, Elliott Wave Principle. Its just as much an artwork like a science nevertheless it truly does grant some amazing insights. I do not even understand what fundamental examination to look at, so I will defer towards your abilities on that entrance. I would like to insert a chart right here for you personally (and any one else who's intrigued) to take a look at:Hence the respond to towards the query of how long and ways in which considerably the equity markets are going to drop this time close to seems to lie with the destiny of your dollar. If ever the stock marketplaces will fall for the subsequent 6 months as you advise, then the dollar rally would past for almost precisely the very same length of time. It will really have to by default, until the romance concerning the chart in some way develops into disconnected. If which were to occur Peyton Hillis Jersey, it wouldn't be something wonderful. It will in all probability be one thing devastating, indicating the stock market and also the dollar falling in tandem. God forbid!My very own get the job done suggests the rally from the dollar could in fact final before possibly April or May likely. That seems to relate pretty intently to your projection on the markets falling for that very same amount of time. Though the depth of one's drop complete can be quite likely to problem the March lows in my opinion. I do not wish that to take place because I say I feel it'll. On the contrary, I along with any other constructive contemplating human being would take pleasure in to observe the S shift solidly to 1800 and further than, but provided that it transpired over the strength of a genuinely strong American marketplace. I really hope you discover the chart appealing and I'd be actual curious to grasp if it might somehow agree with or assist to verify your work.Oct 31 01:50 AMI really don't ponder there is certainly a lot question the greenback "could surprise". The fact is that, you'll find it virtually guaranteed to "surprise" simply because very last time I read through a report on the bullish sentiment for that dollar, it confirmed that sentiment stood at 96% bearish. In my opinion, the actual fact which the marketplaces have rallied for so long and on these suspicious volume action. that is certainly the actual shock. The market was only in a position to maintain up this sort of a sustained eight thirty day period rally because of the FED's absolute refusal to assist the dollar. That as well ought to have actually been thought of the valid surprise. Why did not the FED do a damned point to prop up the buck? Merely because had they done so, the crash from the 2007 highs wouldn't have stopped where exactly it did in March. You can get pressures these days that advocate the dollar could indeed appreciate a amazingly potent bounce. That is most certainly planning to shock, {but if|but when|however Matt Cassel Jersey, if} it does without a doubt rally for that size of time, we're investigating the pretty powerful probability of one's equities marketplaces falling perfectly into next spring, at least. It's virtually guaranteed basically because charts clearly show conclusively that for the reason that 2006, and arguably 2003, the association around the dollar and therefore the equity marketplaces is firmly locked in an inverse marriage. Here's a chart I set alongside one another to prove that stage:Oct 31 01:00 AMThe current market was spooked nowadays? What about final Friday? How about Monday, Tuesday and Wednesday? As I recall, the marketplace fell exhausting on all 4 of those people days on gigantic volume. All they treatment about is usually that just as has generally happened as March, GS will occur swooping in like Superman on crack and protect the day. Truth be told, when the market place bounces on Monday http://www.chiefsjerseynflauthentic.com/Shop-By-Player-Jamaal-Charles-Jersey-c-14_26 Jamaal Charles Jersey, the chances are now in favor within the likelihood that that would be the "correction".
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Mimo, ze po niepowodzenie chwilowka naczelny przebywalismy ze soba na stopie towarzyskiej, czulismy sie w istocie, jakbysmy znali
sie odkad lat.
Odkrylismy, ze mamy ze soba do licha i troche wspolnego. Poruszylismy cala game tematow od momentu sportu i zawodow do
restauracji oraz zagadnien socjoekonomicznych. Rozmawialismy o zmieniajacym sie swiecie. Moc czasu
spedzilismy dyskutujac o tym, iz gros Amerykanow zaoszczedzilo na czas emerytalny zdziebko lub
nic, podobnie podczas gdy bedace tuz przy progu bankructwa stanowe wydzialy ubezpieczen spolecznych a zasilkow. Azaliz od
moich dziatwa bedzie sie wymagalo placenia na emerytury gwoli 75 milionow osob stanowiacych wyz
Dziwilismy sie jednakowoz ludzie zdaja sobie sprawe sposrod tego, podczas gdy ryzykowne jest poleganie na programie
zaopatrzenia emerytalnego.
Podstawowy zgroza Roberta dotyczyl rosnacej luki pomiedzy tymi, co maja a tymi, co nie maja – w
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Powodem, dla ktorego powrocil az do aktywnosci, jest ow tenze strach, kto ogarnia mnie w stosunku do
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Roberta, dzieci spedzaja cale lata w przestarzalym systemie edukacyjnym, uczac sie rzeczy, ktorych nigdy
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text:
- Introduction
- Materials
- Ordering Information
- Characteristics of DG44 Cells
- Thawing and Subculturing DG44 Cells
- Freezing DG44 Cells
- Transfecting DG44 Cells with FreeStyle™ MAX Reagent
- Selecting for Stable Transformants
- Choosing a Workflow
- Genomic Amplification by MTX Selection
- Clonal Selection by Limiting Dilution
- Clone Scale-Up
- Troubleshooting
- References
The OptiCHO™ Express Kit contains the pOptiVEC™-TOPO® TA Cloning Kit, FreeStyle™ MAX transfection reagent, DG44 cells, growth and selection media, and additional reagents for complete transfection of DG44 cells in a serum-free environment and subsequent stable cell line development.
Components of the OptiCHO™ Express Kit
The OptiCHO™ Express Kit includes the following major components:
- pOptiVEC™-TOPO® TA Cloning Kit: A TOPO®-adapted bicistronic plasmid and reagents for cloning of a PCR product containing a mammalian secretion signal and the gene of interest.
- DG44 cells: DHFR-negative, CHO-derived cells adapted to high density, serum-free suspension culture in CD DG44 Medium that are capable of producing high levels of secreted, recombinant protein.
- CD DG44 Medium: Defined, serum-free medium supplemented with hypoxanthine and thymidine to allow growth of DHFR-negative DG44 cells.
- FreeStyle™ MAX Reagent: A proprietary, animal origin-free formulation for high transfection efficiency of plasmid DNA into DG44 cells.
- CD OptiCHO™ Medium: Defined, serum-free medium formulated for selection and growth of DG44 cells expressing DHFR and the recombinant protein of interest.
- The kit also contains OptiPro™ serum free medium (SFM) for optimal DNA:lipid complex formulation, L-glutamine provided separately for increased media stability, and the surfactant Pluronic® F-68 to control shear forces in suspension culture.
Advantages of the OptiCHO™ Express Kit
Using the OptiCHO™ Express Kit for protein production in mammalian cells provides the following advantages:
- DHFR-deficient DG44 cells derived from CHO cells (Urlaub et al., 1983), which provide stable and accurate glycosylation (Sheeley et al., 1997; Werner et al., 1998) and are more likely to yield accurate glycoproteins.
- FreeStyle™ MAX Reagent offers high transfection efficiency of suspension CHO cells with low cytotoxicity.
- FreeStyle™ MAX Reagent, CD DG44 Medium and CD Opti CHO™ Medium are animal-origin free.
pOptiVEC™-TOPO® TA Cloning Kit
The OptiCHO™ Express Kit contains the pOptiVEC™-TOPO® TA Cloning Kit (also available separately). The pOptiVEC™-TOPO® TA Cloning Kit contains the pOptiVEC™-TOPO® vector, a TOPO®-adapted bicistronic plasmid that allows rapid cloning of a PCR product containing a mammalian secretion signal and the gene of interest downstream of the CMV promoter. In the pOptiVEC™-TOPO® vector, the transcription of the gene of interest is separated from the dihydrofolate reductase (DHFR) auxotrophic selection marker by an internal ribosome entry site (IRES), allowing transcription of the gene of interest and the selection marker on the same mRNA.
Using the reagents provided in the pOptiVEC™-TOPO® TA Cloning Kit, you will TOPO® clone your gene of interest into the pOptiVEC™-TOPO® vector, transform the plasmid into One Shot® TOP10 E. coli, and select the appropriate clone prior to transfection.
Experimental Outline
This manual provides instructions and guidelines to perform the following steps:
Thaw and propagate DG44 cells in CD DG44 Medium
Transfect your pOptiVEC® -TOPO® plasmid construct containing your gene of interest into DG44 cells using FreeStyle™ MAX Reagent
Select for a pool of stably transfected cells using CD OptiCHO™ Medium
Perform genomic amplification by selection with MTX
Using limiting dilution, select a clonal cell line that expresses high amounts of your protein of interest
Scale up your high-producing clonal cell line to suit your bioproduction needs
The components of the OptiCHO™ Express Kit are shipped and should be stored as listed below.
TOPO® TA Cloning Reagents
The pOptiVEC™-TOPO® TA cloning reagents are shipped on dry ice in two boxes, containing the cloning reagents and the One Shot® TOP10 Chemically Competent E. coli kit. Refer to the pOptiVEC™-TOPO® TA Cloning Kit manual for detailed description of the kit contents.
Store the TOPO® reagents at -20ºC and the One Shot® TOP10 Chemically Competent E. coli kit at -80°C.
DG44 cells
DG44 cells are provided as one vial shipped on dry ice containing 1 x 107 cells in 1 ml freezing medium containing 10% DMSO.
Important: Upon receipt, immediately store in liquid nitrogen.
OptiCHO™ Express Kit Components
The OptiCHO™ Express Kit components are shipped under various conditions as listed below.
Store components as indicated.
FreeStyle™ MAX Reagent
1 ml
Blue ice
+4ºC, Do not Freeze
OptiPro™ SFM
100 ml
Room temperature
CD DG44 Medium
1000 ml
Room temperature
+4ºC, in the dark
CD OptiCHO™ Medium
1000 ml
Room temperature
+4ºC, in the dark
L-glutamine, 200 mM
100 ml
Dry ice
Pluronic® F-68, 10%
100 ml
Room temperature
Room temperature
Ordering Information
Parental Cell Line
The CHO-S cell line is a stable aneuploid cell line established from the ovary of an adult Chinese hamster (Puck, 1958). CHO cells are commonly used cell lines for transfection, expression, and large-scale production of recombinant proteins.
DHFR catalyzes the reduction of 5, 6-dihydrofolate to 5, 6, 7, 8-tetrahydrofolate, which is essential for DNA synthesis. CHO-derived DG44 cells lack DHFR activity and must be propagated in medium containing the purine precursors hypoxanthine and thymidine (HT) unless the cells are stably transfected with a vector that expresses DHFR. Auxotrophic selection eliminates the need to maintain selection pressure using antibiotics, which could potentially contaminate your protein of interest.
DHFR also functions as a genomic amplification marker for your gene of interest using methotrexate (MTX) selection (Kaufman et al., 1985; Tanaka et al., 2002). See genomic amplification using MTX for more details.
Characteristics of DG44 Cells
The DG44 cell line exhibits the following characteristics:
- Adapted to serum-free suspension growth in CD DG44 Medium containing hypoxanthine and thymidine.
- Demonstrates high transfection efficiencies with FreeStyle™ MAX Reagent.
- Suspension cultures may be transfected in CD DG44 Medium.
As with other human cell lines, when working with DG44 cells, handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Do not thaw and grow DG44 cells in CD OptiCHO™ Medium. Parental or untransfected DG44 cells are DHFR-negative and require supplementary hypoxanthine and thymidine, present in CD DG44 Medium.
Preparing Complete CD DG44 Medium
- All solutions and equipment that come in contact with the cells must be sterile. Always use proper sterile technique and work in a laminar flow hood.
- Supplement CD DG44 Medium to a final concentration of 8 mM L-glutamine and 18 ml of 10% Pluronic® F-68 per liter before use.
- Addition of antibiotics is not recommended.
- CD DG44 Medium is light sensitive. For optimal results, store medium at 4ºC, protected from light.
Materials Needed
You should have the following reagents and materials before beginning:
- Frozen DG44 cells (supplied with the kit; store frozen cells in liquid nitrogen until ready to use)
- Complete CD DG44 Medium (prepared as above; pre-warm at 37°C before use).
- 125 ml polycarbonate, disposable, sterile Erlenmeyer flasks with vented caps (available from VWR, West Chester PA, cat. no. 30180-036)
- Orbital shaker set at 130-135 rpm in 37°C incubator with a humidified atmosphere of 8% CO2
Thawing Procedure
To thaw and establish cells:
- Remove the cryovial of cells from the liquid nitrogen and thaw quickly (<1 minute) in a 37°C water bath.
- Decontaminate the outside of the vial with 70% ethanol. Gently break up any clumps with a sterile pipette tip and aseptically transfer the entire contents of the cryovial into a disposable, sterile polycarbonate 125-ml Erlenmeyer shaker flask containing 30 ml of pre-warmed complete CD DG44 Medium.
- Incubate cells in a 37°C incubator containing a humidified atmosphere of 8% CO2 in air on an orbital shaker platform rotating at 130-135 rpm.
- After 24-48 hours in culture, determine the cell density and viability using the protocol described below.
- Once the culture has reached >1.2 x 106 viable cells/ml, expand cultures using the subculturing protocol below.
Determining Cell Density and Viability
Follow the procedure below to determine viable and total cell counts.
- Transfer a small aliquot of the cell suspension to a microcentrifuge tube.
- Determine viability using trypan blue dye exclusion.
- Determine cell density electronically using a Coulter Counter or manually using a hemacytometer and an inverted microscope.
Subculturing Cells
Passage the cells once the culture has reached >1.2 x 106 viable cells/ml. When passaging DG44 cells, use disposable, sterile polycarbonate 125-ml Erlenmeyer shaker flasks with vented caps containing 30 ml of pre-warmed complete CD DG44 Medium.
- Determine viable and total cell counts.
- Using the cell density determined in Step 1, calculate the split ratio needed to seed each new shaker flask at 2 x 105 viable cells/ml by dilution.
- Dilute the cells in 30 ml of pre-warmed complete CD DG44 Medium to give a final cell density of 2 x 105 viable cells/ml.
- Incubate flasks in a 37°C incubator containing a humidified atmosphere of 8% CO2 in air on an orbital shaker platform rotating at 130-135 rpm.
- Repeat Steps 1-5 as necessary to maintain or expand cells.
After 25 passages, you should thaw a new vial of cells. To maintain sufficient stocks of low-passage cells (i.e., under 25 passages), be sure to freeze aliquots of DG44 cells in liquid nitrogen. See the next section for instructions on cryopreserving cells.
You may freeze DG44 cells directly in CD DG44 Medium with 10% DMSO. We recommend that you freeze cells at a density of ≥1 x 107 viable cells/ml. Guidelines to prepare freezing medium and to freeze cells are provided in this section.
Materials Needed
You will need the following reagents and equipment before starting:
- Complete CD DG44 Medium
- Tissue culture grade DMSO
- Reagents and equipment to determine viable and total cell counts
- Sterile, labeled cryovials
- Sterile, 15 ml-conical tubes
- Automated or manual controlled-rate freezing apparatus
Preparing Freezing Medium
Prepare freezing medium immediately before use.
- In a sterile, conical centrifuge tube, mix together the following reagents for every 1 ml of freezing medium needed:
|Complete CD DG44 Medium
|0.9 ml
|0.1 ml
- Filter-sterilize the freezing medium through a 0.22 µm filter and place the tube on ice until use. Discard any remaining freezing medium after use.
Freezing Cells
- Grow the desired quantity of DG44 cells in shaker flasks, harvesting when the cell density reaches 1 x 106 viable cells/ml. Transfer cells to a sterile, conical centrifuge tube.
- Determine the viable and total cell counts and calculate the volume of freezing medium required to yield a final cell density of 1 x 107 viable cells/ml.
- Centrifuge cells at 1,200 rpm for 5 minutes at room temperature and carefully aspirate the medium.
- Resuspend the cells in the pre-determined volume of chilled freezing medium.
- Place cryovials in a microcentrifuge rack and aliquot 1 ml of the cell suspension into each cryovial.
- Freeze cells in an automated or manual controlled-rate freezing apparatus following standard procedures. For ideal cryopreservation, the freezing rate should be a decrease of 1°C per minute.
- Transfer frozen vials to liquid nitrogen for long-term storage.
Note: You may check the viability and recovery of frozen cells 24 hours after storing cryovials in liquid nitrogen by following the thawing procedure.
You will use FreeStyle™ MAX Reagent to transfect suspension DG44 cells with your pOptiVEC™-TOPO® construct. Refer to the pOptiVEC™-TOPO® TA Cloning Kit manual supplied wit the kit for instructions to clone your gene of interest into the pOptiVEC™-TOPO® vector.
Plasmid Preparation
The pOptiVEC™-TOPO® plasmid must be clean, sterile, and free from contamination with phenol and sodium chloride for transfection into DG44 cells. Contaminants may kill the cells, and salt will interfere with lipid complexing, decreasing transfection efficiency. We recommend isolating plasmid DNA using the S.N.A.P. Midiprep DNA Isolation Kit.
Linearizing the Plasmid
Prior to using the OptiCHO™ Express Kit to transfect DG44 cells with your pOptiVEC™ construct, you may linearize the plasmid. While linearizing your vector may not improve transfection efficiency, it increases the chance that the vector will integrate into the host cell genome without disrupting the gene of interest or other elements required for expression in mammalian cells.
We suggest using Pvu I, which cuts once in the ampicillin resistance gene. Other unique restriction sites are possible. A complete restriction map of pOptiVEC™-TOPO® is available at www.invitrogen.com. Be sure that your insert does not contain the restriction enzyme site you use to linearize your vector.
After digestion, precipitate the DNA, resuspend pellet in sterile water, and re-quantify using your method of choice.
Note: If an appropriate linearization site is not present, you may transfect the circular plasmid. Transfection efficiency will not be affected.
Positive Control
If you performed the control TOPO® cloning reaction to clone the LacZα fragment into the pOptiVEC™-TOPO® plasmid, you may use this plasmid construct as a positive control for transfection. The DHFR gene in the plasmid will allow generation of stably-transfected DG44 cell lines in HT-deficient CD OptiCHO™ medium.
Calculate the number of DG44 cells that you will need for your transfection experiment and expand cells accordingly. Make sure that the cells are healthy and greater than 95% viable before proceeding to transfection.
Materials Needed
You should have the following reagents and equipment before beginning:
- Suspension DG44 cells cultured in complete CD DG44 Medium at 5 x 105 cells/ml
- Purified, linearized pOptiVEC™-TOPO® plasmid DNA containing your gene of interest
- Optional: purified, linearized pOptiVEC™-TOPO® plasmid DNA containing the LacZα fragment, as a positive control
- FreeStyle™ MAX Reagent (supplied with the kit; store at +4°C until use)
- OptiPro™ SFM (supplied with the kit; pre-warmed to room temperature)
- Disposable, sterile, 125-ml polycarbonate Erlenmeyer flasks
- Orbital shaker in 37°C incubator with a humidified atmosphere of 8% CO2
- Reagents and equipment to determine viable and total cell counts (e.g., Trypan Blue, hemacytometer, Coulter Counter)
- Vortex mixer
Optimal Transfection Conditions
To transfect suspension DG44 cells in a 30 ml volume, we recommend using the following optimized conditions:
- Final transfection volume: 30 ml
- Number of cells to transfect: total of 1.5 x 107 cells (cell density at time of transfection should be 5 x 105 cells/ml)
- Amount of plasmid DNA: 18 µg
- FreeStyle™ MAX Reagent: 15 µl
Note: Further optimization of culture volume or transfection conditions is not necessary for stable cell line production.
Transfection Procedure
Follow the procedure below to transfect DG44 cells in a 30-ml volume. We recommend including a positive control (linearized pOptiVEC™ TOPO® plasmid with LacZa) and negative controls (no DNA, no FreeStyle™ MAX Reagent) in your experiment to help you evaluate your results.
- At 48 hours before transfection, pass DG44 cells at 3 x 105 cells/ml in complete CD DG44 Medium. Place the flask(s) on an orbital shaker platform rotating at 130-135 rpm at 37°C, 8% CO2.
- At 24 hours before transfection, pass DG44 cells at 3 x 105 cells/ml in complete CD DG44 Medium. Place the flask(s) on an orbital shaker platform rotating at 130-135 rpm at 37°C, 8% CO2.
- On the day of transfection, perform a viable cell count. To ensure optimal transfection results, viability of cells must be over 95%.
- For each transfection or control, transfer 1.5 x 107 viable DG44 cells to a new 125-ml flask. Add pre-warmed, complete CD DG44 Medium to a final volume of 30 ml. Place flask in shaker until ready to transfect.
Note: Do not spin cells down prior to transfection, as it will decrease transfection efficiency.
- Gently invert the tube of FreeStyle™ MAX Reagent several times to mix. Do not vortex.
- Add 18 µg of plasmid DNA and 15 µl of FreeStyle™ MAX Reagent into 1.2 ml of OptiPro™ SFM and mix gently.
- Incubate the DNA-FreeStyle™ MAX mix for 10 minutes at room temperature to allow complexes to form. Do not incubate for longer than 20 minutes.
- Slowly add 1.2 ml of DNA-FreeStyle™ MAX Reagent complex into the 125-ml flask containing cells while slowly swirling the flask.
- Incubate transfected cell cultures at 37°C, 8% CO2 on an orbital shaker platform rotating at 130-135 rpm.
- At 48 hours post transfection, pass cells into HT-deficient, complete CD OptiCHO™ Medium. Proceed to the next section, Selecting for Stable Transformants
Transfected DG44 cells should be passaged in complete CD OptiCHO™ Medium. To passage cells:
- Determine viable and total cell counts using your preferred method.
- Dilute the cells in pre-warmed complete CD OptiCHO™ Medium to give a final cell density of 3 x 105 viable cells/ml (see Important, below).
- Incubate flasks in a 37°C incubator containing a humidified atmosphere of 8% CO2 on an orbital shaker platform rotating at 130-135 rpm.
- Cells should be passaged in fresh complete CD OptiCHO™ Medium every 2 days for 10-14 days until cell viability increases to >90%.
During selection with CD OptiCHO™ Medium, cell viability could drop dramatically (to <10%) due to the death of untransfected and transiently-transfected cells. To promote the optimal growth of stably transfected cells, keep the culture volume at a minimum of 30 ml.
Positive Control
DG44 cells transfected with the control pOptiVEC™ plasmid containing LacZα will not express your protein of interest but will express DHFR, allowing them to grow in CD OptiCHO™ Medium. Maintaining these cells in parallel with your experimental cells will help you estimate how long it will take to obtain stable cell line formation, generally about 10-14 days.
You can detect expression of DHFR in the positive control cells by western blot using an antibody to DHFR (Rabbit polyclonal anti-DHFR, Sigma cat. no. D0942).
Protein Production
To check for production of your protein during stable cell establishment, you may take an aliquot of growth media and perform SDS-PAGE, protein-specific ELISA, or the bioactivity assay of choice to determine that your cells are producing your protein of interest.
When you have a pool of stably-transfected cells, you should cryopreserve several aliquots of the pool.
Next Steps
Depending on your protein production experimental needs, refer to the next section Choosing a Workflow to determine the next steps.
You will have a population of stably-transfected DG44 cells expressing your protein of interest at various levels. For most bioproduction applications, several clonally-derived cell lines producing your protein of interest are desirable for screening. However, the growth and optimization of each clone is dependent upon the integration locus of the plasmid, the response to amplification using MTX, and the nature of the protein.
Depending on your protein production needs, the time and effort required to generate clonal, high-producing cell lines is also variable. Several common pathways from stable pool to clone scale-up are outlined below.
Points to Consider
When choosing a workflow from the options above, you should consider the amount of protein needed for your application, your available resources, and the amount of time it will take to obtain your clonal, high-producing cell lines.
Additional media and other products may be purchased separately from Invitrogen.
- To perform genomic amplification using MTX selection to obtain a population of cells expressing high levels of your protein of interest.
- To perform limiting dilution to obtain single clones expressing high levels of your protein of interest.
- To scale up your clones for protein production, Clone Scale-Up.
Methotrexate (MTX) is a folic acid antagonist that is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase, which binds to DHFR and inhibits its activity.
If MTX is present in the medium, cells compensate by increasing the DHFR copy number in the genome to overcome inhibition by MTX. Since the gene of interest is integrated into the same genetic locus as DHFR, the gene of interest is amplified as well, leading to increased production of the protein of interest (Kaufman et al., 1985; Tanaka et al., 2002). MTX (as methotrexate hydrate) is available from Sigma (10 mg, catalog number A6770).
MTX is toxic to the skin, eyes, and respiratory system. Wear suitable protective clothing, gloves, and eye and face protection when working with MTX. Refer to the product MSDS for complete precautions.
Preparing 1 mM MTX
To prepare a 1 mM MTX stock solution:
- Dissolve 10 mg MTX in 22 ml of PBS.
- Filter sterilize the solution through a 0.22 µm filter.
- Store in 250 µl aliquots at -20ºC.
- Important: To make complete CD OptiCHO™ Medium with MTX, you will need to use complete CD OptiCHO™ Medium (prepared with L-glutamine) plus the required concentration of MTX.
- Important: Prepare media containing MTX fresh each time. Do not re-use prepared MTX media.
Preparing Media with MTX
Using the sterile, 1 mM MTX stock solution (prepared as described above), prepare media with the appropriate amount of MTX.
The recommended selection concentrations per round of selection are 50 nM, 100 nM, 250 nM, 500 nM, 1 µM, 2 µM and 4 µM. Be sure to make enough media containing MTX for your application.
- For each clone, spin down cells and aspirate old medium.
- Seed cells at a density of 2 - 5 x 105 cells/ml in 30 ml of media containing MTX in 125-ml flasks.
- Incubate flasks at 37ºC/8% CO2 with shaking at 130-135 rpm for 14-21 days or until viability >90%.
- Optional: Repeat steps 1-3 with the next highest concentration of MTX.
Expected Results
In the experiment below, a pool of stable transformants were created after transfection of DG44 cells with pOptiVEC™-TOPO® vector constructs containing erythropoietin (EPO). The cells were exposed to increasing amounts of MTX, and EPO expression was determined by ELISA after each completed round of amplification (results shown on day 5 after seeding the cells at 2 x 105 cells/well).
The amount of EPO produced increases 50-fold after 7 rounds of genomic amplification using MTX
Next Steps
Because MTX selection produces a population of cells producing high amounts of protein, you will have to perform clonal selection by limiting dilution prior to clone scale-up.
Cloning Medium Considerations
- To grow DG44 cells under adherent conditions, use your clone optimization medium of choice, such as Gibco® Custom formulations F17 or CD-CHO-A.
- Because the growth and protein production of each clone are variable, you may need to optimize clonal selection conditions by adjusting the number of cells/well, the cloning medium, and various media supplements.
- Depending on how many clones you wish to screen before scale-up, you may increase the number of limiting dilution plates accordingly. The number of clones obtained from a 96-well plate is variable, depending on the experiment.
- Determine the number and viability of your cells.
- Make a 10-fold serial dilution using an appropriate volume of cloning medium such that you will obtain 1 cell per 100 µl.
- Pipette 200 µl of diluted cells into each well of a 96-well plate to obtain ~2 cells/well.
- Incubate plates without agitation at 37ºC with a humidified atmosphere of 5% CO2.
- Single cell clones will be readily apparent after 14 – 21 days post-plating (see Important below).
After single-cell derived clones appear in 96-well plates, let them grow undisturbed, without changing the medium, at 37ºC in a 5% CO2 atmosphere until they are >75% confluent before proceeding to scale-up. Failing to do so will result in loss of clones due to low cell density.
Next Steps
You should isolate single clones for further testing. Once individual clones have been isolated and tested for high levels of protein production using your method of choice, they can be subjected to gradual MTX amplification or clone scale-up.
Materials Needed
You will need the following materials before beginning:
- Clones in 96 well plates that are >75% confluent
- Cloning medium of choice
- Complete CD OptiCHO™ Medium
- Sterile tissue culture dishes (96-well, 48-well, 24-well, 12-well, and 6-well) and sterile 125-ml polycarbonate flasks.
- Optional: TrypLE™ Express, if cells do not detach with gentle pipetting.
Add 100 µl fresh cloning medium to wells with single colonies and pipette gently until cells in the colony detach.
Optional: If cells do not detach easily with gentle pipetting, aspirate media from wells, add 50 µl of TrypLE™ Express and incubate for 5 minutes at 37ºC. Add 50 µl of fresh cloning medium and pipet up and down until cells detach.
- Transfer the entire amount of cells to 48 well plates and add an additional 250 µl of fresh cloning medium to each well.
- Incubate plates undisturbed at 37ºC, 5% CO2 until they are 80-90% confluent; usually 2-3 days depending on the clone growth.
- Proceed to the next volume (i.e. 24-well).
- Repeat Steps 1-5, transferring cells into a larger format tissue culture plate with each successive round.
- Once cells are cultured in a 125 ml flask, switch medium to complete CD OptiCHO™ and incubate cells at 37ºC 8% CO2, with shaking at 130-135 rpm.
Note: If MTX selection has been performed, include the same amount of MTX used in the final selection (e.g. Include 1 µM MTX if 4 rounds of selection were performed with a final concentration of 1 µM MTX)
Next Steps
Prior to optimizing protein production, the appropriate cell stocks should be frozen down. Protein production experiments can then be optimized using different culture conditions, or scale-up can be continued to meet your bioproduction needs.
Culturing DG44 Cells
The table below lists some potential problems and possible solutions that may help you troubleshoot your cell culture experiment.
No viable cells after thawing original vial
Cells not stored correctly
Order new cell stock and store in liquid nitrogen. Keep in liquid nitrogen until thawing.
Incorrect thawing medium or method
No viable cells after thawing stocks
Cells not frozen correctly
Follow protocol to freeze cells.
Incorrect thawing medium
Cells grow slowly
Incorrect growth medium
Shaker not set up properly
Shake on an orbital shaker at 130-135 rpm in 37°C incubator with a humidified atmosphere of 8% CO2.
Medium is foamy
Keep shaker speed at 130-135 rpm.
Cells too old
Use healthy DG44 cells under passage 25; do not overgrow.
Cell culture clumpy
Provide agitation of the culture, a regular and frequent cell passage schedule, and maintenance of cells at recommended densities.
Kaufman, R., Wasley, L., Spiliotes, A., Gossels, S., Latt, S., Larsen, G., and Kay, R. (1985) Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase in Chinese hamster ovary cells. Mol Cell Biol 5, 1750-1759.
Puck, T. (1958). J. Exp. Med. 108, 945.
Sheeley, D. M., Merrill, B. M., and Taylor, L. C. (1997) Characterization of monoclonal antibody glycosylation: comparison of expression systems and identification of terminal alpha-linked galactose. Anal Biochem 247, 102-110.
Tanaka, H., Tapscott, S., Trask, B., and Yao, M.-C. (2002) Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cells. PNAS 99, 8772-8777.
Urlaub, G., Kas, E., Carothers, A., and Chasin, L. (1983) Deletion of the Diploid Dihydrofolate reductase Locus from Cultured Mammalain Cells. Cell 33, 405-412.
- Werner, R. G., Noe, W., Kopp, K., and Schluter, M. (1998) Appropriate mammalian expression systems for biopharmaceuticals. Arzneimittelforschung 48, 870-880.


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Loutra is a traditional Cretan village surrounded by olive groves, just set back from the north coast of the island. A holiday home in Loutra offers guests calm and serenity, with several unspoilt sandy beaches close by, making it perfect for couples on a summer escape. At the same time, Loutra is close to some of the island's larger resorts, making a villa in Loutra the perfect base for families in Greece.
There is no better way to appreciate the fantastic Cretan weather than to simply laze on a sun-lounger and soak up the sun. Most Loutra holiday villas have their own private pool, which is ideal for cooling off when the heat gets too much.
If you don't want to spend your entire holiday by the pool, there are plenty of sandy beaches all within a 5-minute drive of Loutra. Here you can swim or snorkel and some beaches also have water sports on offer such as jetskis. There are also glass-bottom boat trips operating nearby which let you see the local marine life without getting your feet wet.
The area around Loutra is very picturesque, with some great walking trails running close by the village. Visitors may also wish to explore the small village of Loutra itself, a traditional Cretan village with its own small shop and bakery, producing fresh local treats every day.
Only short drive from Loutra are several historical sites, including the Arkadi Monastery, the Arsani Monastery, Melidoni Cave, and several Venetian sites, any of which make for a great half-day trip.
There is also plenty around Loutra catering to families, with a children's play centre only a short drive away, as well as activities such as go karts, pitch and putt and crazy golf.
Although Loutra itself is a tranquil village, the larger town of Rethymnon is only a 5-minute drive. Here visitors can walk around the old town, dominated by a medieval fortress, and meander around the traditional fishing port. The town has lots of great waterside cafes in which to try traditional Cretan fare, as well as having several bars and clubs for those looking for some night-time fun.
Loutra enjoys a fantastic Mediterranean climate. Summers are long and hot, with temperatures sometimes reaching over 30C and very little rain, perfect for a beach holiday. Winters too stay very mild, with temperatures rarely dipping below 10C and often hitting 18C, even in January, making a Loutra villa the perfect place to escape the cold British climate.
Crete is served by Heraklion Airport (HER), 53km from Loutra. This receives daily flights from the UK including those operated by Thomson, easyJet, Monarch, Jet2 and flythomascook. All the major car hire operators can be found at the airport or there are shuttle buses available to transfer you to your Loutra self-catering accommodation.


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Sage Student Bistro - Institute for the Culinary Arts
- Address:
5730 North 30th street Building 22
Omaha, NE 68111Neighborhood:
Downtown / Old Market
- Cuisine:
Contemporary AmericanPrice: $30 and under
Sage Student Bistro - Institute for the Culinary Arts
- Dining Style: Fine Dining
- Website: http://resource.mccneb.edu/Bistro/
- Phone: 4024572321
- Hours of Operation:
Dinner Reservations accepted: Monday - Thursday: 6:00pm - 8:00pm
- Payment Options: Discover,MasterCard,Visa
- Cuisine: Contemporary American
- Executive Chef: Oystein Solberg/Tim O'Donnell
- Dress Code: Business Casual
- Accepts Walk-Ins: Yes
- Additional Details: Chef's Table, Non-Smoking Restaurant, Personal wines welcome, Personal wines welcome (corkage fee applies), Wheelchair Access, Wine
A word from Sage Student Bistro - Institute for the Culinary Arts
Sage Student Bistro is a student run operation that runs concurrently with the school quarter.Continued
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Sage Student Bistro - Institute for the Culinary Arts reviews from OpenTable diners
16 reviews since June 4, 2014
- Food4.8Service4.8
- Ambiance4.8Noise LevelQuiet
fit for foodies (12)special occasion (12)romantic (8)great for lunch (7)good for groups (7)neighborhood gem (7)kid-friendly (2)notable wine list (2)great for brunch (2)hot spot (1)great for outdoor dining (1)Show More
- Don't miss out on this place!BlackCloudDined on 9/25/2014What a find! I've lived in Omaha for 10 years and never heard of this place. I was looking for something interesting plus special for our 1 year anniversary. I will say we were not disappointed. The menu is very limited and same with the drink selection but put all that aside. It is the experience that counts. The food was great. I had the trout and wife had the chicken. Both dishes were amazing. Our waiter Stanley was incredible. He explained in detail the menu and was very attentive. We were there on a Thursday and the noise level was very low with nice music in the backgroud. I definitely recommend this place.Special Features:I would recommend this to a friend.
- Sage Student BistroKeystonegirlsDined on 9/24/2014There were 6 in our group. We were very, very impressed with the overall high quality of Sage Student Bistro! Everything was wonderful - it's a beautiful restaurant and the food was just amazing. It's not an expansive list but we all found something we really liked. I can't wait to take my husband. It's a very upscale feel and would be perfect for a date night or a family dinner. Wow, were we impressed.Special Features:I would recommend this to a friend.
- A Special PlaceLovedItDined on 9/23/2014Great food, great ambience, great service, as long as you're patient with the fact that it's a teaching restaurant where there may be a glitch here and there (I needed to ask for a soup spoon, for instance, but the cauliflower soup was delicate and wonderful).Special Features:
- Creative and satisfying menuOpenTable Diner Since 2012Dined on 9/18/2014We found the food well presented and satisfying. The staff continued to bring out one surprise after another - baskets of bread, little tastes, candies, spritzers. They were very gracious and accommodating when several of our guests arrived late. We were delighted with the quality, service and ambiance. Well beyond expectation.Special Features:
- culinary school excellentBigdDined on 7/10/2014Visited during the fine Dining class' Prix fix menu. Excellent food and excellent service. Very nice meal for a good price.Special Features:I would recommend this to a friend.
- AwesomeWhtimiswhtugtDined on 7/10/2014My whole experience was amazing...the food was incredible...everyone was so attentive I loved everything about this place...I can't wait till fall so I can share the experience with someone elseSpecial Features:I would recommend this to a friend.
- Very nice experienceGreatAxiomsDined on 7/7/2014My wife and I tried out the Sage Bistro for a date, and we were not disappointed. The food was artistically presented and very delicious. In our opinion the $30 was a great bargain. I really appreciated our talk with one of the chefs (Jarrod Lane), and we found the whole evening relaxing and enjoyable. By far my favorite piece was the rabbit. There was one piece of lighter meat that was wrapped in crispy rabbit bacon, and I have no other word to describe it than exquisite. I approached the meal with some trepidation, since it was part of a school project, but everything was done to perfection.Special Features:
- The prix fixe menu was divinelisa968ksDined on 7/3/2014We had a very large group and were waited on hand - and - foot by our server valerie. Food was spectacular, and so was the presentation. We met with the student chefs and their student manager. Very pleasant. Ambience was quiet and classy. Prices were quite reasonable. Will be returning.Special Features:I would recommend this to a friend.
- Great timeKreJDined on 7/1/2014My boyfriend and I had an amazing dining experience at the Sage Bistro. Kinesha Roach and her partner cooked the prix fixe and it was absolutely divine. The ambiance was simple perfect and everything we ate was pleasing to our pallets. It was a nice date for us and was the first of many prix fixe experiences. We had such a good time he treated me to a prix fixe experience a couple of weeks later at Sullivan's for our birthday. Each time I've eaten at Sage, it is clean, the food is delicious, and the staff is professional. I recommend it to many people on a continual basis.Special Features:I would recommend this to a friend.
- true gemsusiecatDined on 6/26/2014The food at Sage Bistro easily equals a "professional" restaurant. We ordered and shared two different appetizers, entrees and desserts, and each was spectacular. I understand that the menu, chefs, and servers all change as the students rotate through their coursework, so perhaps this was luck of the draw for us. We will definitely be back. p.s. don't miss the creme brûlée, if it is available.Special Features:I would recommend this to a friend.
- The following 236 reviews are from the Sage Student Bistro - Institute for the Culinary Arts reviews archive.
- Loved the experienceOpenTable Diner Since 2007Dined on 5/14/2014Went there with our book group - food was excellent, as was the service. We all had the prix fix meal and it was great!
- Great ServiceJetwhipDined on 5/13/2014The service was on point and very attentive. The food was cooked wonderfully and I enjoyed each bite. The instructors seem they really care about the students!Special Features:I would recommend this to a friend.
- Great little place; upscale dining.kikoDined on 5/12/2014I've never been to this bistro before. I wasn't sure what to expect, but I was really impressed by how clean and beautiful the place was. Between each course, the silverware was replaced. It was lovely. And the food was great, too. It was nice to see the students at metro have a chance to show their talent, and it's nice for the customers to be able to come back another time and have different options.Special Features:
- entertainment for out-of-townersOpenTable Diner Since 2010Dined on 5/1/2014Having gone through the fine dining course at Metro, I notice things. I was taught by the best that you always serve the women first and from the left and clear from the right. That didn't happen. Also, the presets were just dropped on the table in front of us instead of placed in their proper places. The standards are lower than the last time I attended. The servers were knowledgeable about the food and it was very good.Special Features:I would recommend this to a friend.
- Good food and service.CuriousConfusiousDined on 5/1/2014The ambience is fit for special occasions. The service is great and prices are fair. Also, it is a great program that prepare students for the real world.Special Features:I would recommend this to a friend.
- Looking for a Gem?1culinaryfanDined on 4/22/2014If you are looking for a gem you shouldn't miss Metropolitan Community College's Sage Bistro. This restaurant is run by students who have been taught by, and are watched over by some of the best chef/instructors in the United States. The ambiance is quiet and lovely with many windows, opening to an outdoor eating area. The service was impeccable. Explanations of the delicious and beautifully presented Escoffier inspired prix fixe dinner were included with each of the five courses. This made our meal an education, as well as an opportunity to meet some of the students. It was a wonderfully relaxing experience, with enough time between courses not to feel rushed. All in all, Sage Bistro is a valuable find.Special Features:I would recommend this to a friend.
- Where students learn from customersbuglerDined on 4/17/2014The wine service was somewhat unsure. The pork entree my wife had 2 styles of pork. The two pieces of pork (same size) were not cooked to the same level of 'doneness'. The cabbage wrapped shredded pork that came with her entree, the shredded pork was dry, not moist. My perogi stuffed with oxtail meat was good.Special Features:I would recommend this to a friend.
- Great foodPsk10Dined on 4/10/2014Food was awesome, presented very well, unique and very tasty.Special Features:
- eating at schoolcareich2Dined on 4/8/2014different not what I'm used to very good though and surprisingly full when I left the wait staff was very friendlythey were also very informative too another thing that I really liked was that they had beer from a local companybut the best thing that I hadtherewas the desertSpecial Features:I would recommend this to a friend.
- Wonderful Find...OpenTable Diner Since 2014Dined on 4/2/2014I loved the atmosphere of the whole dining experience. The goose terrine was amazing. All of the dishes in our party were fantastic. My pork tenderloin with apples and cabbage was very well balanced with flavors and textures. We also loved the coffee cake for dessert. We will definitely be back and cant wait to try some more wonderfully cooked food.Special Features:
- Portions too small and theMichaelDined on 4/2/2014Portions too small and the appetizer was cold. We ordered the bisque with mussels and scallops. The bisque was cold and so were the mussels and scallops. When we mentioned it to the waiter, he returned to say that the chef says that is how it's served. If this was the case, we were not impressed. We spent 80.00 for this experience and returned the appetizers which we were not given credit for and we left hungry and ended up going to M's Pub afterwards. We read the reviews before giving this place a try and we were expecting a not so perfect experience since it was a learning for students, but we didn't expect what we got for the price. We do not recommend this place when you can spend your money on a much better experience elsewhere.
- Service was excellent exceptKD66Dined on 3/27/2014Service was excellent except for reception at the door. No one appeared to be at the door to receive and seat guests at first. After being seated I noticed another guest experiencing the same difficulty. This may be a function of the positioning of the hostess.Special Features:I would recommend this to a friend.
- First NightgoodfoodDined on 3/24/2014We had a lovely meal with the students doing their best to overcome first night jitters of the new quarter. The food was very good and we loved being a part of the education of the students. We hope to return at the end of the quarter when everyone has perfected their roles. We love to see young people become confident in what they love.Special Features:I would recommend this to a friend.
- Fun dining experienceSuzyQDined on 2/20/2014I have been to sage for lunch, but this was my first dinner experience. The presentation was spectacular, and the favors matched the presentation. The menu is always changing. My only two complaints were that service was slow and the server didn't thoroughly explain the dishes. My sister is gluten free, and they were very accommodating.Special Features:I would recommend this to a friend.
- Omaha's Hidden Gem!futurenurseDined on 2/17/2014Very few people know about Sage Bistro. My friend recommended it to me and my husband and I had out delayed Valentine's Day dinner there. We both had an absolutely amazing experience! Quality, taste, and price of food was outstanding. The service and the atmosphere were great! We both enjoyed dining out at Sage Bistro very much. Definitely coming back and recommending to friends.Special Features:I would recommend this to a friend.
- On a par with the bestKenMDined on 2/13/2014I've had lunch at the Sage Bistro numerous times, so I knew dinner would be good, but our experience on February 13th far exceeded expectations. From the sweet salty oyster amuse bouche to the light herbaceous salad contrasted with foie gras and goat cheese to a spectacular artichoke ravioli to perfectly cooked lamb two ways to a strawberry shortcake thingy that had the cleanest, most intense strawberry flavor I've ever had, not to mention the delightful lagniappes of an intermezzo and candies, these eats were just terrific. I was once a restaurant critic and my wife and I travel and eat out a lot. This meal ranks with the best. Look out Grey Plume and Boiler Room, they're coming for you. A bargain at twice the price.Special Features:I would recommend this to a friend.
- Valentines Diner67CountryDined on 2/13/2014Wife had the trout. Was not turned on by the head attached. Students diner had the chop and sauce was tangy for me.Special Features:I would recommend this to a friend.
- The Short Rib was the mostOpenTable Diner Since 2014Dined on 2/11/2014The Short Rib was the most dissapointing dish. It was dry, poor texture, lack of flavor. Definitely not expecting it to be ground ribs. The duck was excellent. Those that ordered the pork and beans found it good, but nothing particularly outstanding about it. Two of the four couples had been to lunch the same week and raved about the food they had. They were particularly disappointed in the the dinner. We will try lunch next time. Will probably try dinner once more next semester.Special Features:I would recommend this to a friend.
- DuckOpenTable Diner Since 2013Dined on 12/19/2013I had duck for the first time and it was delicious. Overall, simply a great experience. Lastly, the desserts were great as well as the small complimentary dishes that came by.Special Features:I would recommend this to a friend.
- A treasure!anonymousDined on 12/17/2013If you've never dined at a culinary school you've missed a treat! The food is not only delicious but beautifully presented and perfectly served by young chefs who take great pride in their work! Try it. You'll love itSpecial Features:I would recommend this to a friend.
- Uh.May.Zing!OpenTable Diner Since 2011Dined on 12/16/2013I came with three of my friends for a birthday celebration. The facility is beautiful, the staff was very professional and friendly and the food was outstanding. I have eaten in some of the best restaurants in the country (Avec in Chicago, The French Laundry in the Napa Valley) and Sage Student Bistro is right up there with the best of them. The flavors were incredible, the presentation beautiful and the overall experience was wonderful. I will recommend this to anyone looking for a unique dining experience in Omaha and I will be coming back again.Special Features:I would recommend this to a friend.
- A wonderful experience allwanderer13Dined on 11/7/2013A wonderful experience all around. It won't be the last time . . .Special Features:
- always learningbuglerDined on 11/6/2013Both the staff and the guest can learn at this restaurant/school. Just watching the staff being taught tableside by an instructor on how to decant wine, can teach the guest a few things. While the food is always good, and presentation can be be seen as creative/fresh, it seems the plates are just barely warm. If you get talking to a table companion, or one person plate is coming out slower than the rest of the table, the hot food could always be served warmer.Special Features:I would recommend this to a friend.
- Dining Experience JoyOpenTable Diner Since 2011Dined on 11/4/2013The Sage Student Bistro is simply an overall exceptional dining experience! When you are seated in this well designed modern space, I challenge you to recognize it as a culinary learning environment on a community college campus. In short, the food was very tasty, exceptionally presented, and effectively portioned. The service was timely, efficient, pleasant, and above all - not snooty! The wine list is small but ample. Other restaurants could learn a thing or three. What is going on here is fantastic. The city of Omaha is lucky to have this place. Bravo to the students and to the teaching team. If you call yourself a foodie, this is your next place. I will indeed return again - and again.Special Features:I would recommend this to a friend.
- You will be amazed!JaniceDined on 11/4/2013A great neighborhood find with good food and class!Special Features:I would recommend this to a friend.
- The MCC culinary studentsSpikeDined on 10/30/2013The MCC culinary students do a great job. I usually have the prix fixe. Portions are small so you don't feel stuffed. Wine list is short but I have always found a wine I liked.Special Features:I would recommend this to a friend.
- It was just a pleasant relaxingVivienneDined on 10/17/2013It was just a pleasant relaxing restaurant. Our waiter was a very nice young man. I wish him all the luck in the world for his future. The food was great. I came away very full and satisfiedSpecial Features:I would recommend this to a friend.
- Dining at Sage Student BistroJousieDined on 10/17/2013There were 8 in our group and we loved the quiet homey atmosphere. Food was divine and the experience supperb. Food was so much fun to try many different taste and different presentations. We loved every minute of our time together and the personal attention we were given and the beautiful grounds outside with the lovely herbs draping over the walls. Cannot wait to do out door dining as the setting is perfect and so welcoming. Staff was lovely and so enduring, waited on it hand and foot and I am recommending it to many and already have and will continue. Plan to get back there for lunch very soon. It is a long ways for us to drive, about 30 minutes but the drive is worth it. Thank you so very much, we will come again!!Special Features:I would recommend this to a friend.
- Outstanding service and food!Aldee257Dined on 10/14/2013Outstanding service and food! It was a magical night. The Student chefs did a wonderful job. Our waitress was attentive without being overbearing!Special Features:I would recommend this to a friend.
- Great meal, Great priceBigDDined on 10/14/2013Excellent experience for a culinary school cafe staffed by students in the dining room and the kitchen. The food was as good as a couple high end places in town and much better than many others. The lamb was grilled perfectly, nice rare as ordered with a good sear that gave it a nice bite.Special Features:I would recommend this to a friend.


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News for friends of the Arthritis Foundation, Great West Region
July 2013
Articles This Issue
School is Back in Session for those with Arthritis
The Arthritis Foundation's public education forums provide attendees with unique lessons about the future of arthritis and more. Read how.
Why I Volunteer
Mary Norton cycles on behalf of all those she loves that are affected by arthritis. Read her story.
John Vernachio Named Vice President of Research
The Arthritis Foundation, National Office appoints biotechnology expert to helm research efforts. Learn more.
Going the Distance
Kids with arthritis often have to travel great distances to get the care they need. Read one family's story.
Smoke Signals
Local humanitarians help their community, one rack of ribs at a time. Read more.
Top of their Towns
2013 marks a banner year for Arthritis Walks in the Great West Region. Find out the Region's top teams.
The New KidsGetArthritisToo.org
A new, comprehensive website launches for families affected by juvenile arthritis. Discover its resources.
School is Back in Session for those with Arthritis
The Arthritis Foundation's public education forums provide attendees with unique lessons about the future of arthritis and more.
One of the most vital services that the Arthritis Foundation provides to people with arthritis are education forums. These half-day or full-day forums are packed with educational presentations related to arthritis, resource tables from community partners, question and answer sessions and exercise demonstrations.
Especially when paired with the Arthritis Foundation's Programs for Better Living, educational materials, website resources, interactive online tools or our community seminars, the education forums can provide successful outcomes to help people live with the daily challenges of having a chronic disease.
Through 15 education forums from Alaska to Colorado, over 49 presentations were made to hundreds of people thus far in 2013.
The topics at these education forums often include sessions on nutrition, disease overviews, coping skills, alternative and complementary therapies, medication reviews, exercise techniques, research updates, and disability rights.
"Participants at our education forums often gain clarity about their disease and can often be surprised by the basic facts related to arthritis or treatments. There's often an 'a-ha!' moment to be had at an Arthritis Foundation education forum," says Lisa Fall, Chief Mission Officer for the Arthritis Foundation, Great West Region.
Stuart Kassan, MD, FACP, is a rheumatologist and presenter at many local education forums. "The Arthritis Foundation patient education seminars are an excellent way for patients to get the latest information regarding the most effective treatment options for people living with the chronic pain of arthritis. This information helps empower people and enables them to make informed decisions about their health care," he says.
Panelists and presenters, made up of rheumatologists, orthopedists, physical therapists, complementary medicine experts and mental health professionals, to name a few, often have valuable takeaways of their own. Many presenters learn a lot from the participants and their questions.
"The education forums give me the platform to answer questions in a group setting. This helps patients because many of them have similar questions. This helps de-bunk the myths and misnomers. Factual information is empowering and helps people feel more at ease with what they are facing," comments William J. Peace, MD, a presenter at one of the education forums.
"Presenting at education forums with the Arthritis Foundation helps us reach more people and improve the lives of those living with arthritis. It's a win-win-win for our practice, the Foundation, and, most importantly, the patients," says Todd Miner, MD.
Everyone's arthritis story is different, but the medical and expert presenters at the education forums can center their information to anyone's individual needs.
The education forums attract people from all walks of life, with all forms of arthritis. Many participants bring their primary caregivers and friends or family members so they can all learn the latest ways to manage the disease and explore, and relieve, the impact on the whole family.
"Arthritis doesn't just affect the person diagnosed with arthritis, it affects the whole family," says Fall. "We often hear that the caregivers or family members walk away having learned just as much at an education forum as the person with arthritis."
When participants attend patient education forums, they gain knowledge and support, meet others who share their challenges and discover new tools to add to their arthritis management arsenal.
Find out more:
Discover upcoming education forums in your area on our Events page.
Explore many of the topics presented at education forums through the Arthritis Foundation website informational sections.
The Arthritis Foundation offers a series of online videos related to arthritis care and management. Watch these videos to learn more.
"Why I Volunteer..."
Mary Norton
Mary Norton cycles on behalf of all those she loves that are affected by arthritis.
Mary Norton has been a dedicated Arthritis Foundation volunteer and cyclist for nine years. Mary is a rider and team captain for Team Nana as part of the California Coast Classic Bicycle Tour. The California Coast Classic is the Arthritis Foundation's eight-day cycling event that takes riders from San Francisco to Los Angeles to raise awareness and support for the Arthritis Foundation.
A lifestyle bicyclist, you can often find Mary pedaling around Oakland and Berkeley on her hybrid or road bikes. Mary stepped off of her bike for a few minutes to sit down with AFeatures to discuss her volunteerism and family connection to arthritis.
Name: Mary Norton
Location: Oakland, California
What is your connection to arthritis?
There's certainly a family connection!
My mother died from the effect of rheumatoid arthritis at age 82, which was three years ago. By that point, the joints in her hands were so destroyed that she no longer had any joint connections. She couldn't garden and she had stopped driving because her hands didn't have enough strength to hold the steering wheel.
My mother probably had rheumatoid arthritis long before her actual diagnosis. She was always pushing forward and knew that if she stayed active and kept moving that it would prevent her arthritis from becoming worse.
My daughter Sarah was diagnosed with juvenile rheumatoid arthritis when she was 12 years old. She is 25 years old now. Sarah had major flares of her disease, which we were fortunate to be able to get under control with medications each time. Because of how prevalent the field of medicine has been in Sarah's life, she's now studying integrative and alternative medicine at the National College of Natural Medicine in Portland, Oregon.
My sister also has rheumatoid arthritis, and was diagnosed on the exact day I began riding the California Coast Classic two years ago.
How did you first become connected to the Arthritis Foundation?
I was in a bike shop in Berkeley, California and saw a pile of brochures for the Arthritis Foundation's cycling event, the California Coast Classic Bicycle Tour.
I'm a lifestyle bicyclist. I commute to work and travel everywhere on my bike. Because of that, I knew that this was an event that I could do and I wanted to support the cause because of my family connections to arthritis.
I called the Arthritis Foundation and they connected me to a coach to help me train for the ride. That was the beginning of my involvement.
What were some of the ways that the Arthritis Foundation has supported you?
The biggest one is Arthritis Today magazine. I find this to be an incredible resource for people with arthritis. I read every issue from cover to cover. I work at a senior center and I always bring in my copy to share with everyone.
The local office of the Arthritis Foundation here in San Francisco is a font of information and support. They've helped me realize things like the importance to keep moving.
How have you been involved with the Arthritis Foundation as a volunteer over the years?
I rode in my first California Coast Classic in 2005. This year, 2013, will mark my ninth ride.
I ran the Marin County Jingle Bell Run/Walk in 2012 for the first time. I made first in my age division, which I call the "old lady prize".
We now hold an Arthritis Foundation Exercise Program in the senior center where I work, and are planning to offer the Arthritis Foundation Tai Chi program soon as well.
I am always promoting the Arthritis Foundation and its work. My goal is to constantly get the word out about arthritis, especially about juvenile arthritis. I show the post-California Coast Classic ride video at my senior center. It really inspires the people there to see the great lengths people go to so that they can support the Arthritis Foundation. I also show the slideshows for our local juvenile arthritis camps. It always blows people away to learn that kids get arthritis, too.
What makes a good volunteer?
A good volunteer stems from when people have a connection to arthritis, whether it is inherited or learned.
Volunteering makes you feel like there's something you can do for those with arthritis. The term 'take control' is in the motto of the Arthritis Foundation and volunteering allows you to do just that.
What are some of your favorite Arthritis Foundation volunteer experiences?
There are countless moments that I hold on to and consider inspiring as a volunteer.
Seeing all those California Coast Classic cyclists that are riding with arthritis, and seeing them persevere… that's very powerful. When my daughter Sarah (pictured right with Mary) rode the California Coast Classic, her wrists would hurt so badly by the end of the day. There's another member of the ride that just keeps on moving the whole time because she knows that if she rests too long inflammation will set in and she may not be able to move again that day.
When we hold our kick-off dinner for the ride, they ask all those riders with arthritis to stand up. It's very moving to me.
Was there ever another Arthritis Foundation volunteer that impacted your life?
Jeff Bass is one of the riders on the California Coast Classic. He has imbued me with the concept of being an ambassador for the Arthritis Foundation at all times. Whether you are at work, at home or at play, there are always opportunities to share the story of people with arthritis and the Arthritis Foundation. Jeff has taught me that.
Jerry Kruse is another volunteer that has significantly impacted my life. Jerry is one of the coaches for the California Coast Classic. He leads training rides. On these training rides he ensures that everybody feels welcome and part of the group. He makes sure that everyone has a good day, and offers meaningful, practical advice and support. He is a super coach, and always keeps us going on long rides. He builds everybody up so that each and every person is ready for the California Coast Classic. Jerry is everyone's inspiration.
Find out more:
The Arthritis Foundation, Great West Region offers two premiere cycling events.
The California Coast Classic is an eight-day ride that takes place September 28-October 5, 2013 and travels down the coast of California, from San Francisco to Los Angeles. More information can be found at www.AFCABikeClassic.org.
The Amgen People's Coast Classic takes place September 8-13, 2013 and travels down the Oregon coast from Astoria to Brookings. Two, four and six day rides are available. For more information, go to www.thepeoplescoastclassic.org.
John Vernachio Named Vice President of Research
The Arthritis Foundation, National Office appoints biotechnology expert to helm research efforts.
The Arthritis Foundation announces the appointment of John Vernachio, Ph.D. as vice president of research effective April 15, 2013. In his new role he will provide leadership and direction to further the Arthritis Foundation research program and the organization's commitment to finding a cure and better, safer treatments for the nation's leading cause of disability. Vernachio will succeed Dr. John Hardin, who will assume a new role within the organization developing and implementing flagship initiatives in osteoarthritis.
"John Vernachio is a world-class scientist and director in biotechnology with a proven track record," says Arthritis Foundation Chief Mission Officer, Jennifer Griffiths, Ph.D. "I am confident that under his leadership the Arthritis Foundation will continue its legacy in driving targeted research initiatives that will push us toward a cure."
Vernachio brings to the Arthritis Foundation extensive research leadership and expertise, serving as a researcher and technical director in the biotechnology sector for more than 20 years. Most recently, he served as vice president of biology for Inhibitex, Inc. Earlier, he held supervisory and leadership roles in several biotechnology companies including Baxter Healthcare and Valentis, Inc. In addition, he is an author on over 20 peer-reviewed scientific articles, numerous scientific presentations and is a co-inventor on 10 patent applications.
A graduate of the University of Vermont, Vernachio earned a Bachelor of Science degree in Animal Science in 1984. He received a doctorate in biochemistry with a concentration in immunology and molecular biology in 1989 from Johns Hopkins University and completed his post-doctoral training in the Department of Immunology at The Research Institute of Scripps Clinic in San Diego in 1991.
Find out more:
Visit the Arthritis Foundation's research page to find out the latest arthritis research initiatives.
Going the Distance
Kids with arthritis often have to travel great distances to get the care they need.
Audra Olson knows the drive like the back of her hand. Every month, sometimes more often, Audra faces a three hour drive through rain, snow, traffic and other obstacles to get her daughter the medical care that she needs.
Audra's daughter Libby Rinke (pictured, left) was diagnosed with aggressive polyarticular juvenile rheumatoid arthritis at age 10. Libby's hands, ankles, feet, hips and knees are affected by inflammation and pain because of the disease. Now 11, Libby is on her third biologic medication, the first two medications having failed, and starting to see positive results.
Libby requires the medical attention that only specialists can provide. In order to get it, the Olson/Rinke family realized that they would need to travel outside of their home town of Wenatchee, Washington, located in the central part of the state.
"We only have one adult rheumatologist in our area that sees kids," Audra says. "We needed to go to Seattle Children's Hospital to get Libby the best, and necessary, care."
The 300 mile round-trip journey leads them over two mountain passes, takes at least three hours to complete one way and costs over $150 in gasoline and food costs.
Audra estimates that they have traveled to Seattle Children's Hospital at least 27 times since Libby's diagnosis. Not only does Libby need to see her pediatric rheumatologist at Seattle Children's Hospital every three months, but she also has monthly physical and occupational therapy appointments there, as well as medication infusions (pictured at right) and appointments with other related specialists such as gastroenterology. On average, Libby has six hours of appointments at each visit.
"We are at Seattle Children's a lot for regular appointments, and that doesn't even include emergency appointments if Libby is flaring or if her meds aren't working and we need to change her medications," Audra says.
She continues, "I'm lucky to have the vacation and sick time to be able to take off of work for each of these appointments, but it still uses up most of my time off from work to go back and forth to Seattle."
The Arthritis Foundation is seeking ways to alleviate this burden of travel on families by increasing the number of pediatric rheumatologists. Currently, there are less than 250 board certified, practicing pediatric rheumatologists in the United States and about 90 percent of those are clustered in and around large cities. Pediatric rheumatology has one of the smallest numbers of doctors of any pediatric subspecialty.
Of those children with juvenile arthritis, only one-fourth see a pediatric rheumatologist due to their scarcity. The other 75 percent of juvenile arthritis patients see either pediatricians (who tend not to be trained in how to adequately care for juvenile arthritis) or adult rheumatologists, who aren't trained to deal with pediatric issues, whether it's the stunted bone growth that can result from arthritis and its treatment, or the unwillingness of an adolescent to take his or her medicine. Furthermore, the diseases that are common in children can be very rare in adults, so a rheumatologist may have rarely, if ever, had occasion to diagnose and/or treat those related diseases and co-morbidities. There are currently eleven states that do not have a single practicing pediatric rheumatologist and seven states with only one pediatric rheumatologist.
The Pediatric Subspecialty Loan Repayment Program was authorized by Section 5203 of the Affordable Care Act (ACA) in March 2010. The program, which would be administered by the Health Resources and Services Administration, would incentivize training and practice in pediatric medical subspecialties, like pediatric rheumatology, in underserved areas across the United States. The program would offer up to $35,000 in loan forgiveness for each year of service for a maximum of three years. The program was authorized for $30 million for fiscal year 2010 through fiscal year 2014, but has yet to be appropriated any funding.
In the fiscal year 2013 budget, President Obama requested $5 million dollars to fund the Pediatric Subspecialty Loan Repayment Program. Then, in July, 2013, the Senate Appropriations Committee approved a spending bill for fiscal year 2014 that proposed $5 million for the Pediatric Subspecialty Loan Repayment Program.
To receive funding in fiscal year 2014, the House of Representatives must also include funding in their proposed fiscal year 2014 spending bill, which the House is currently drafting. To help alleviate the current shortage of pediatric rheumatologists in the United States, the Arthritis Foundation is urging Congress to appropriate $5 million to fund the Pediatric Subspecialty Loan Repayment Program in fiscal year 2014.
The Pediatric Subspecialty Loan Repayment Program is at a critical juncture. Arthritis advocates can make a difference by reaching out to their Representative and requesting their support. When you do, you'll be helping children with arthritis, like Libby, get the specialty care they need closer to home.
Find out more:
Find out the latest updates on this issue, or send a pre-drafted message to your Representative requesting their support for the Pediatric Subspecialty Loan Repayment Program.
Smoke Signals
Local humanitarians help their community, one rack of ribs at a time.
There's smoke rising in the north, and it smells good.
It's July 6, 2013 in North Pole, Alaska. Hundreds of runners, walkers, strollers and dogs have gathered for the 2013 Arthritis Foundation Jingle in July, a 5K run and walk to raise support and awareness for arthritis in North Pole, Alaska. Participants don festive attire, including elf ears, Santa suits and jingle bells on their shoelaces.
The race gun goes off, and the runners and walkers start the race. 3.1 miles later, they begin crossing the finish line. The top fundraising teams file into the VIP tent, where a special treat awaits them – a full service trailer serving up classic Texas barbecue. Yup, Texas barbecue in North Pole, Alaska!
Joyce and Jewel's BBQ serves barbecue, free of charge, at charity events.
They charge no fees for providing food to non-profit organization community events. They donate all of the costs of supplies, food, travel and their time to the non-profit organization they are serving. Brisket, ribs, chicken, sausage, beans and more are all on the menu when they serve hundreds of people at community events each year.
Husband and wife team Larry and Tina James (pictured left) started Joyce and Jewel's BBQ in 2010 as a way to honor their mothers, Joyce James and Jewel Vivens, who had both passed away. Their goal was to create a legacy for their loved ones and "send smoke to heaven for fallen angels".
Their motivation was further strengthened when their daughter Avonna, who was born with a rare liver disease, passed away in March of 2011. Tina and Larry expanded their mobile barbecue trailer and began offering their donation of time, spirit and food at many more community events.
Despite their other full-time life commitments (Larry is Superintendent, 354th Contracting Squadron at Eielson Air Force Base near Fairbanks, Alaska), Tina and Larry's first love is the work of Joyce and Jewel's BBQ.
Larry pronounces, "For me, if I'm not out serving people and being a blessing to other people than I'm not living my life right. We're always looking for opportunities to give back."
One of those opportunities came when Larry found out about the Arthritis Foundation's Jingle in July.
"I saw a banner on a fence for the Jingle in July, and called the Arthritis Foundation to offer our help," says Larry. "Jingle in July was an awesome experience. It warmed our hearts. We sponsored the VIP tent, which was an area for those people that raised the most money at the event."
Larry continues, "It was great to be able to share our story at Jingle in July. When people go through tough times in life, like when they have arthritis, it can be good to hear a story like ours of how you can change tough times into something to help others."
"We want people to know that they can make it through, too."
Larry's Dad and Aunt are both affected by arthritis, making his volunteerism at the Jingle in July a bit more personal.
And Larry's favorite menu item of Joyce and Jewel's BBQ? "It's gotta be 'Avonna's Awesome Beans'. My daughter helped develop the recipe and it always gets rave reviews."
Find out more:
To learn more about Joyce and Jewel's BBQ and see photos of their tastiest barbecue, visit their website at www.joyceandjewelsbbq.com.
Top of their Towns
2013 marks a banner year for Arthritis Walks in the Great West Region.
The Great West Region 2013 Arthritis Walk season is almost at a close, with one event remaining. Combined, nine Arthritis Walk events had over 4,427 participants and raised over $460,000 through 5,454 donors.
We would like to take this opportunity to thank all those who walked, donated, attended and spread the word about their local Arthritis Walks.
Top Arthritis Walk Teams in the Arthritis Foundation, Great West Region:
1. DAC Rheumatology (Denver) - $13,885
2. Team BAAA (San Francisco) - $12,022
3. SOAR Rheumatology (San Francisco) - $11,871
4. Jessters (San Francisco) - $10,810
5. 5GIRLS1D (Bellevue) - $9,553
6. Andy & Katie's Team (Denver) - $7,916
7. Zack Attack (Tri-Valley) - $7,887
8. Kids and Teens Get Arthritis Too! (Tacoma) - $7,562
9. CrazyBonez (Tri-Valley) - $4,819
10. Emma Heaton (Salt Lake City) - $4,780
11. The J Team (San Francisco) - $4,691
12. Team Emanuel (San Francisco) - $4,505
13. Team Ellie (Denver) - $4,120
14. Team Esella (Tri-Valley) - $4,100
15. Joint Effort (Denver) - $4,005
16. Valor Christian (Denver) - $3,815
17. Movers and Shakers (San Francisco) - $3,548
18. Crescendo Bioscience (San Francisco) - $3,301
19. Team Scrappi (San Francisco) - $3,244
20. R.O.A.R. (Really Outrageous Arthritis Racers) (San Francisco) - $3,049
21. Shelby's Stars (Vancouver) - $3,048
22. Grand for the Cure (Denver) - $2,926
23. Team Emma (Eugene/Springfield) - $2,875
24. Dom's Amazing Race (Denver) - $2,845
25. Ashlyn's Allstars (Salt Lake City) - $2,836
26. Team Jazz (Tri-Valley) - $2,710
27. Anna's Angels (Denver) - $2,581
28. Team Ashley (Tri-Valley) - $2,565
29. Viva Aviva (Tri-Valley) - $2,532
30. Classy Claire's Walkers (Denver) - $2,516
31. Reidyns Rebellious Renegades (Vancouver) - $2,514
32. Walk This Way (Tri-Valley) - $2,427
33. Lively Lupus Lapsters (San Francisco) - $2,402
34. Team Libby (Bellevue) - $2,382
35. Rollin' Homies (Bellevue) - $2,357
36. Oma's Flare Fighters (Denver) - $2,332
37. Sams peeps-Walmart 2925 (San Francisco) - $2,325
38. Hand Surgery Associate's Team (Denver) - $2,306
39. Keep up the pace (Salt Lake City) - $2,293
40. Laurie Jones Neighbors (San Francisco) - $2,231
41. Brynne's Brigade (Denver) - $2,230
42. JOINT EFFORT (Tri-Valley) - $2,212
43. Peachey's Pack (Denver) - $2,007
44. Team Ankylosaurus (San Francisco) - $1,966
45. These Boots Were Made for Walking (Tri-Valley) - $1,962
46. Arthritis Fighters (Eugene) - $1,928
47. KATELYN'S CREW (Tri-Valley) - $1,837
48. ROAD Clinic @ part of FMG (Tacoma) - $1,771
49. Moving Together (Denver) - $1,700
Team Webster (Tri-Valley) - $1,700
50. Team Slocum (Vancouver) - $1,630
51. Kassan's Krew (Denver) - $1,595
52. Team Bankles (Denver) - $1,585
53. Team Nelson (Denver) - $1,580
54. The Peter J. Seippel Foundation & Friends (San Francisco) - $1,372
55. Team Believe (Vancouver) - $1,350
56. Bethesda Sole Mates (Colorado Springs) - $1,300
Find out more:
Ready for more community involvement? Jingle Bell Run/Walks are coming up in November and December across the Region. Find your local event.
The Southern Oregon Arthritis Walk will take place on Saturday, October 5, 2013 in Medford, Oregon.
The New KidsGetArthritisToo.org
A new, comprehensive website launches for families affected by juvenile arthritis.
Introducing a new website from the experts you trust!
Everything you need to live better with juvenile arthritis, now in one place.
• Find information, tips and other great resources
• Ways to connect and share with other families
• Learn about medications and treatment options
• Read the latest about research breakthroughs
• Parent and kids stories, local events and more
Get Connected
Find your local Arthritis Foundation office to get connected to programs and events.
Find out about all of our volunteer opportunities on our Volunteer Resource Page or fill out a volunteer application.
Feedback or ideas for AFeatures future stories? E-mail email@example.com
Read back issues on the AFeatures Archive page.
Subscribe to receive future publications


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