text:
Extreme Personal Finance: Eating Well on One Dollar a Day
Published on - June 9th, 2010 (Modified on - June 13th, 2010) (by J.D. Roth)
Last Thursday, Ron Lieber (who writes the "Your Money" column for The New York Times) posted an innocuous little tweet:
This person will have book deal & Today show slot in 5 minutes. RT @marypilon Personal finance blogger eats on $1/day. http://bit.ly/aeGlmC
To translate into plain English, Jeffrey from the Grocery Coupon Guide blog undertook a little experiment last month. In response to a challenge from his sister he "ate well" on just a buck a day, thanks largely to his awesome shopping skills and couponing prowess.
Because I love stories of extreme personal finance, and because I haven't highlighted one in a long time, I decided to take a closer look; I spent an hour reading about his project. Holy cats! This fellow's shopping abilities are insane.
If you don't have time to read the entire series, at least check out day one, in which Jeffrey documents the things he bought to get the project started. He photographed everything he purchased and every receipt, and describes exactly how you might be able to find similar discounts. It's great stuff. (Near the end of his introductory post, he gives a sort of table of contents for the project so that you can jump to the day you want.)
On that first day, he picks up two boxes of instant oatmeal, some cream cheese and some sour cream, ten apples, four boxes of wheat thins, a jar of peanut butter, two cans of pork and beans, a bag of rice, and two packages of tortillas. And he spent just $4.49 for all of this.
Here's Jeffrey's list of the ten thing he learned while eating on $1 a day for a month:
- Grocery shopping is a game. If you're willing to learn the rules of the game, you can save big bucks.
- You can eat more than junk food on a dollar a day. Yes, more money lets you make healthier choices, but you can still make good choices on a tight budget.
- Drugstores can be a great place to get free food. Because it's so easy to save money playing the drugstore game, you can often score food for free.
- If you don't know what a catalina coupon is, you don't know about the most powerful discounts available. These coupons, which print at the cash register, can offer tremendous savings.
- Generic and store-brand items aren't as cheap as you'd think. Their regular price may be less, but you're not going to find coupons and discounts on them like you will on big national brands.
- Most people don't know how to shop to save. They decide what they want and then go to the grocery store to buy those things. To truly save, you need to build your meals around what's on sale (and what you have coupons for).
- It's possible to donate a lot of food while only eating on a buck a day.
- It doesn't take nearly as much time as you'd imagine. Yes, there's a learning curve, but once you know what to do, it's easy.
- Finding coupons is the key. The Sunday newspaper inserts are great, but Jeffrey says that the coupons you find in the stores themselves are often even better.
- Anyone can do it. And once you learn, you'll save big bucks every time you shop.
On the final day of his project, Jeffrey summarized his purchases. During the month of May, he spent $27.08 to purchase $597.96 worth of food. And because of the goofy rules he agreed to with his sister, he ended up donating a bunch of stuff to his local food bank. (The last post is entertaining because of the mistake he makes and then corrects; it had me laughing out loud.)
I like that Jeffrey has a realistic attitude about this project. While he thinks that anyone can do the same thing he's doing, he also recognizes that living on a tight food budget puts you in a precarious position: "The problem with eating on a limited budget, when the unexpected happens…is that you can't just stop into a fast food joint and grab a bite."
Jeffrey was so pleased with the challenge that he decided to continue it. For the past week, he's been chronicling his continued efforts to eat well for less. (Though he's loosened some of the arbitrary restrictions he agreed to when he was trying to prove a point to his sister.) I think it's fun that his readers have started to give him tips and suggestions for finding other great bargains, which helps him keep his costs even lower!
Note: Here are some past GRS stories about extreme personal finance: One month as a freegan, How to pay off your mortgage in three years, The man without money, and the king of extremism, Don Schrader, who lives on $10 a day. Also welcome Lifehacker readers!
This article is about Food, Funny Money, Shopping
In Canada, we get the shaft because all coupons have legalese at the bottom that clearly states "This coupon may not be combined with any other offer." That means that you can use one coupon only per product…not mix and match coupons and offers like he did to create free or surplus situations.
The best we can ever do is to combine a very good store sale with a single coupon.
Too bad…
I feel very ashamed right now.
I have never in my life used a coupon, nor to I know where to find them. My grocery store doesn't give coupons when you buy (the ones he called "catalina"), I have no clue where to find a paper that has coupons, and I've never found coupons in my store.
I can only shop from that one store since there isn't any other… But still, I should be able to get some coupons, right?
I already tend to get the 50% off bread that's in the bin that day and freeze it (just because it's almost stale doesn't mean it can't freeze) but it looks like there is so much more you can do!
My mind is just completely boggled. Where does he find all these coupons? He did mention something about computers, does that mean you need a printer? Because I don't have one or access to one. Apart from the library that prints stuff for a quarter, but wouldn't that end up costing more than I'd save with the coupons?
Anyways, I've been wanting to use coupons for a while, but I just don't get where people find them.
I'm sure I wouldn't be able to spend as little as he did, because I'm vegetarian and allergic to milk, and that seriously lowers my options, but I should be able to save SOME, right?
My husband and I eat very well, and spend between $100 and $200/month on food. By well, I mean, no processed food, fresh veg and fruit, GOOD cheese, and so on. Some meat–not much–and seafood. We don't use coupons and do have a small garden.
The key for us is stockpiling and Big Lots. It's not all that hard, either. Just a bunch of habits. Tonight we're having Thai coconut rice with various vegetables–I may add a few shrimp.
#25 above mentions saving $4000/yr. The important number is not how much you save, but rather how much you spend.
"Healthy, unprocessed natural/organic foods are occasionally on sale, but there are never coupons for them."
This is not true in my experience. I am a vegetarian, eat mostly "natural" foods, and I use coupons all the time on food I consider "healthy" (although this is a relative term!). I can't remember the last time I went grocery shopping without at least 5 coupons. The food co-op near my house has it's own coupon booklet that is published monthly and only includes coupons for natural products that they sell there. I often find printable coupons for the foods I like, or I will sign up for the mailing list of a company and get coupons from them in the mail.
Here are just a few actual examples of coupons that I have used recently:
-$1 off Kashi granola bars
-$1 Cascadian Farm organic frozen vegetables
-$1 off Muir Glen organic pasta sauce
-75¢ off Nasoya tofu
-$1 off Cuties oranges
-$1 off 2 Earthbound Farms organic salad greens
-$2 off 2 Organic Valley milks
Again, I guess it's possible that this is considered unhealthy by other people, but I really believe that almost anyone can find at least a few coupons to use at the store, if they are interested in doing that. You can't get organic stuff for free most of the time, but you can definitely still save money.
@Katie, I believe you. I think those who reflexively say it's impossible to eat healthy and use coupons really just don't want to take the time to use coupons. Which is fine – I don't myself very often – but they should cop to it.
And those who bagged on Jeffrey for being a junk food junkie … wouldn't you love to look in their kitchens? Don't be holier than thou, y'all. When you were a single twentysomething guy/girl, was every meal Alice Waters-worthy? I'll bet it wasn't.
Chacha, other than the in-store coupons actually at Whole Foods… where around LA do you get coupons for healthy food?
The stuff we get in our weekly mailers does not include organic frozen veggies, organic valley milk etc. Occasionally there will be something Kashi (but Kashi is still processed food, and the granola bars are pretty high in sugar), and occasionally I'll see something for Horizon, which is one of those organic companies that the independent agencies don't rate highly. But I have yet to find a useful coupon around here. I looked at that webpage that was posted the last time there was a coupon discussion and nothing on our grocery list was on it.
We do not have a newspaper subscription– is that where all these amazing coupons for things we actually buy come from?
I'd gladly use coupons for things we buy if I could find them. The first three months here I poured through them. I looked again after the last couponing post. Nada. No vale la pena.
I don't usually buy the newspaper, so that's not where I find mine. What I usually do if I like a product is go to their website and look for printable coupons, and then I sign up for their email newsletter. Often this results in new printable coupons being emailed to me periodically. These websites helped me get started:
p.s. I don't buy Horizon either… Check out
http://www.organicvalley.coop/coupons/ and http://www.stonyfield.com/coupons_offers/index.jsp
Yes you can eat those organic, super healthy things at incredible discounts… going on what #25 "forty2″ said:
"I got 10 6oz cups of organic Greek yogurt for $10 last week"
I too bought several of those containers of Greek yogurt for $1 each (but at Kroger, not WF). However, I used $.30 off coupons on them, which were then doubled, making each yogurt cup only $.40. So, if I got my 10 for $4. I'm about to start making my own, which will reduce the cost even more! I also recently bought a container of Smart Balance margarine for $.06.
Basically, Jeffrey was just trying to point out that most people can significantly reduce their food spending, even while eating healthily. Sure, if you truly limit yourself to $1/day, it is not going to be the absolute healthiest of diets, but up that just a tiny bit, and you can eat very well for very little.
Bravo Jeffrey, I really enjoyed reading that series. Thanks J.D. for sharing it.
You mentioned my comment about saving $4,000 a year. On average, we spend a total of $300 a month on groceries, toiletry items, as well as paper/cleaning products. (This also includes a few bottles of wine to support our habit.)
Man, I thought I was doing well on $150 a month. This guys grinds me into the ground.
Absolutely amazing!
I recently found that buying healthy food actually costs less than the cheap nasty generic canned food. Now when ever I look at my receipt, I check to make sure I have not paid any Goods & Services Tax (which is exempt on fresh food in Australia). It's the quickest way to shave 10% of your groceries.
@57, Katie
Thanks! That is the first useful couponing info I've gotten. Especially since we do regularly buy Stonyfield and Organic Valley (though out here I'm hooked on Clover milk when we're at WF).
Wow – he got all of that for $4.49??? I can't buy a jar of peanut butter for less than $2.00 (and that's the smaller size). Wheat Thins are $2.75 for the box I buy.
Lucky is the person who can find such low prices – I sure can't!
I just got done reading Jeffrey's blogs for the past 4 weeks. Very interesting and inspiring. A lot of us are thinking, "if he can do that for $1 a day, think what I could do for $1.50 or $2.00 a day".
Thanks for adding this post JD. I've read a lot about frugality over the past 20 years, and most of what I read is tame and repetitive. It's nice to see something out-of-the-ordinary.
As a side note- I just used amazon.com's grocery delivery service and they included a bouquet of flowers with it! That's right, a bouquet of flowers!!! All the prices were about what I was paying at the outrageously priced safeway and QFC near my place, so the savings was in the time that I can spend on doing homework. (Single dad in college = BUSY!!)
I'm a little late to the party, but I wanted to point out there are TONS of hard core couponing blogs and forums out there. Many of these people have been doing it for years, but it's become much more visible lately due the the economy and more people needing to cut back. I have tried to get into it, but I find that the time is prohibitive for me. (People say it only takes 1-2 hours a week–I must be slow because I could never get it down to less than 3-4!) You do have to clip coupons from the paper, but most come from internet printables. There's even a thriving business for coupons on ebay! (crazy, I know) And to those people who think it is stealing, the coupons are put out by manufacturers to promote their products, encourage people to try new items, etc. Stores get reimbursed for the coupon values, and they are very familiar with the ways they can be used and still choose to accept coupons. No one is being "stolen" from.
That said, it is possible to eat healthy this way too. Maybe you can't do it on $1/day, but you can certainly do it on way less than most people currently spend. It's true the best deals usually come on processed foods, but if you pick and choose how you shop you can still cut your bill quite a bit. For instance, there's a blogger out there (Frugalista) who spends $80/week for her family of 4, and she buys all (or mostly?) organic/sustainable meats, lots of organic fresh fruits and veg, wine, etc. Even with my limited time investment, I've gotten nearly-free whole wheat pasta, frozen veggies, organic milk, yogurt, etc. And to get really technical, the overages mentioned are how a lot of people get "free" stuff–if using a coupon on another item gives you overage of $1, you can use that dollar to buy some organic apples for example, essentially getting them for free.
The point is, even if you want to only shop at Whole Foods you can use coupons and shop the sales to cut back. One poster above said it's not how much you save it's how much you spend, but I don't think it's either. It's how much you cut back and/or fit within your budget. If you normally spend $600/month and want to spend less, cutting back to $500 is still a great step. When my husband and I were single we spent TONS on food because we love to cook and eat, but that wasn't sustainable for us. We now want money to save for our son's college, fix our leaky roof, pay for our medical bills, etc. We have other priorities and don't want to spend $600/month on food like we used to! So we've cut back, focused on cooking at home more, incorporated more meatless meals, use cheap but wholesome ingredients like beans, rice, etc. I would say we eat very well, and coupons help us do that. I know we could save much more if I could get my act together and invest more time, but for now I'm content with spending around $200/month by using coupons on a limited basis, shopping the sales, menu planning, etc. And I am not criticizing the people who save by buying more processed foods–if that's what works for your household more power to you. I'm just saying even a crunchy, first-world foodie like myself can cut back on her groceries!
It's all about priorities. It just annoys me when people get judgmental and sanctimonious. Appreciate your fortunate position in the world and admit that you choose to spend extra money (hopefully because it brings you joy)–just don't pretend it isn't possible to cut back.
What a hoot! This guy must have sooo much patience and determination!!! Me, I'd have to be pushed to the wall to go to that much trouble and effort. But it's mighty nice to know that it actually CAN be done.
When I first saw your post, I thought "Wheat Thins, canned beans, and rice a healthy diet do not make." But when you read his stuff, you see he managed to score fresh produce like apples and carrots, too. It's very interesting.
@ Jen: I don't think it's "judgmental" to note, as Nicole does, that finding coupons for healthy foods is no easy trick. I've tried couponing and found that unless you're into highly processed foods (I'm not–I cook almost everything from scratch, not out of "sanctimony" but because I can't eat the amount of salt and sugar that are in processed foods), there are few coupon opportunities. For example, I wouldn't buy Wheat Thins, not because I don't like them but because they contain three different kinds sugar (regular sugar, invert sugar, and malt syrup); and I think it's ten times cheaper to buy a bag of beans and cook your own than to eat beans out of a can.
He's not suggesting that you do $1 a day. I think the moral here is that if we pay attention, develop a little OCD, do our homework, and really get into it, it can be done. I kid, I kid, Really, for the rest of us it serves as inspiration to slash our own budgets by a quarter or so at least. Every little bit counts.
I have heard of extreme couponing before. In fact, the papers like to push that part of their offerings when trying to get people to buy their papers. I can't say I have spent as little as $30 a month on groceries, but for my husband and I, it probably comes to about $80 to $100 a month. That is because we wait till all our other bills are paid and we go with what is left. But then, God blesses in different ways.
When I lost my job I did not go grocery shopping for 45 days. (At day 30, I bought a dozen eggs).
I just learned to get very creative with what pantry and frozen items I already had!
No coupons necessary!
I have been following Jeffery since very early in his experiment and have completely enjoyed each and every post!
The thing I am seeing here is that way too many people keep saying how couponing means eating junk. The thing is, it's just not true! If many of you took the time to read his story you would see that pretty much through this ENTIRE experiment he has not once eaten just plain old white bread, he has only eaten "healthier" breads. He has not eaten sugar filled cereals, however he has had corn flakes (not frosted flakes), cheerios (not honey nut), and kashi ONLY. Again, if you take the time to read about it you will see that he went through more produce in one month than many families do.
Is his budget/meals extreme? Definitely! Thats the point!
By couponing he not only saves money on certain products but he creates an overage (where you actually EARN money for taking a certain product between the sales price and coupons) and that is how he is able to purchase fresh fruits and vegetables, and generally healthier options. ALSO, because of these deals he has been able to GIVE HUNDREDS of dollars in food and toiletries to various food banks in his area. That alone makes this experiment so worthwhile!
It's ok if couponing isn't for you. No one is trying to force anyone into doing something they don't want to do-but he is bringing awareness to the dire need so many of our local food banks are experiencing due to our economy.
So, instead of knocking it just take a few minutes to read over what he has done.
No such thing as a free lunch. Couponing is a good illustration of "socialization of costs".
I.e., a private benefit paid for at public expense. One such cost is the time and aggravation wasted by all those throughly-annoyed people waiting in line behind the ( obscenity-deleated ) couponer.
Very interesting.
I find it hard to do that stuff where I live. We don't have great coupons and we definitely never have double/triple coupons like some places do. Most of the coupons I find are for fairly new items or items I wouldn't really buy.


text:
P ower Pressure Kleen LLC is a family owned business in Mesa, Arizona. We have been in the contracting business since 2003. We are licensed, bonded and insured contractor in Arizona. We just started this Solar Panel Kleening service to the public in 2013. One thing that is nice about Power Pressure Kleen LLC is we ONLY do solar panel cleaning. We do not do windows, power wash concrete, remove graffiti ect. We SPECIALIZE IN SOLAR PANEL CLEANING. We are here to give you the facts and keep your system clean and maximizing your solar panels. We do not sell any other services so we will never bother our customers with services or products that are irrelevant.
When we show up to your to your property, you will have experienced employees walking on your roof with the utmost care and responsibility a customer should expect. All chemicals used on your solar panels are biodegradable, plant and pet friendly. Every customer gets before and after pictures emailed to them after each Kleening services. You have no need to make appointments for an estimate, we should have all the info we need so your proposal will be sent promptly.
Solar panel companies require your panels to be cleaned several times a year for maximum output and general maintenance. Due to the climate, Arizona solar panels are exposed to a number of elements which can prevent them from providing maximum power.
Tree blockage, bird waste, nests, dirt, airplane fuel waste and hard water -- just to name a few. On average you can lose 5-30% of the energy from your panels as a result of these factors. With a basic cleaning several times per year, you can help prevent this power loss.
Picture will be taken of your panels before cleaning. Cleaning process is performed as per manufacture's specifications. Final picture are taken of cleaned solar panels. All water applied is deionized, which means it is stripped of all hard chemicals, metals, positive and negative electric ion charges, making a spot free wash and rinse.
If your car, house, and boat are maintained on a regular basis, why is your most profited asset not?? Of all your assets this one does not depreciate, it is marking you money. So why not make sure its in top performing condition? On the average it costs the consumer $18-$ 30 a month to make sure all is performing right ( depends on panel size ). So ask yourself ???? Is and will it be worth it. We say YES.
Per your manufacture warranty panels should be clean & maintained on a regular basis.
Yes we are very experienced roof walkers, we don't just spray water from the ground, we brush down each & every panel EVERY Kleening.
Due to home owners not cleaning panels in years, this process will take a few washes to completely remove residue buildup. Due to manufacture requirements only a light detergent shall be used when Kleening.
Your first cleaning due to not being cleaned on a regular basis will need a lot of attention and regular cleanings will help keep them Kleen.
Due to the hard tap water, just spraying them from the ground does not Kleen them, you are leaving a film of residue behind depending on water conditions. That is why we use DI water.
Unless you would like to see us Kleen your solar panels, no you don't. When we are finished you will receive your email w/pictures and the invoice will follow if you are not home at the time of Kleening. We take CASH, CHECK, VISA,MASTER CARD, AMERICAN EXPRESS & DISCOVER.

subset: commoncrawl


text:
About This School
In 2011, Maybury Elementary School had 18 students for every full-time equivalent teacher. The Michigan average is 18 students per full-time equivalent teacher.
Student Ethnicity (2011)
Students (2011)
Students Per Teacher (2011)
Female/Male (2011)
Subsidized Lunch (2011)
District Spending (2010)
Grade 3 Reading Performance
Maybury Elementary School Reviews
Maybury Elementary is one of the best schools my children ever attended, I had my kids in both Detroit Public Schools (Maybury Elementary school / Thurgood Marshall Elementary School / Emerson Elementary School) and Anchorbay Public Schools (Francois MacOnce Elementary School) and I honestly have to say the bad rep DPS gets by the media is so far fetched from reality. Only thing I dislike about the school is the fact I asked several times for a print out of the breakfast and lunch menu but both the teacher and principal failed to fulfill my request.
Maybury Elementary School PhotosThere are no photos for this school yet. Be the first to add a photo.
Washington Virtual Academies
Tuition-free online school for Washington students.


text:
Diet Drugs Work: Why Won't Doctors Prescribe Them?
Re:No, they don't work (670 comments)
I wish
I remember when I realized that not only had a chosen to take the stairs because the elevator was taking too long, but I had run up the last flight.
I disagree about only allowing special people to get treatment, but I can totally validate the intangible of losing a bunch of weight after having been stuck for a couple years.
It is all very subtle and nuanced. Slashdot, alas, is not a good forum for intelligent discussion.
The problem, as I see it, is that we are all speaking from our experience. As an obese person, who has struggled with the disease all my life, it is easy for me to understand that it is a disease. I also have the benefit of having seen the research and spoken with the researchers. I have the experience and the knowledge, so the truth is obvious to me.
But I confess I have little sympathy for people with chronic pain. If I take the time to think about it, I can imagine what it must be like to be in agony all the time, but in the moment I just think "suck it up, we all have crosses to bear."
Seeing the uninformed here is a good reminder for me.


text:
Skip Navigation
Log in with Facebook
Travel Photography
Photos tagged as parks
Winter Bird
No comments yet. Be the first to comment on this photo!
To comment on photos, you need to login first.
Have you forgotten your username or password? Retrieve Your Login Details
35 Netherlands Antilles featured photos
13 Falkland Islands featured photos
92 Antarctica featured photos


text:
There are many reasons why a couple might decide to get married by themselves and I am privileged to have photographed a large number of Just You Two weddings over the last few years.
There is something wildly romantic about a Just You Two wedding, and of course, I absolutely love this kind of wedding. Apart from the intimacy between the couple, who have no guests to worry about and no timings to keep to, the thing I love most is the time I have with the newlyweds, in the amazing landscape of the North West Highlands, creating stunning images.
There are lots of Just You Two weddings for you to look at – just go to the Tag Cloud, hit the Just You Two button, and they should all appear, as if by magic!
If you are planning a Just You Two ceremony, please get in touch – I'd love to hear from you.


text:
Mount Titlis Day Tour from Zurich
We've selected the best candid trip photos submitted by our users to help you decide if the Mount Titlis Day Tour from Zurich is right for you. Make the most of your next trip to Zurich with Viator's exclusive Photo Galleries.
Click here for tour details, schedule and pricing
Mount Titlis
Mount Titlis
October 2008
City of Zurich downhill
September 2008
Dana A
Taking a LONG, beautiful gondola ride to the top of Mount Titlis.
Mount Titlis
September 2008
Enjoying ice skating at Titlis.
Gaurav & Mayur
September 2008
No words to describe. What are you doing here :)
Enjoying Ice Flyer
September 2008
Enjoying Ice Flyer.


text:
Categories > Celebrities > My Chemical Romance > it happens
needles0 Reviews
"hey look theres something else" I said looking at the door, Frank the bastard better no come down.
"what?" she asked.
"is it gonna be awkward if I tell you I have feelings for you?"
"no because I have feelings for you too" she said her cheeks filling up with the little blood she had.
"really?" I asked feeling like an idiot
"cool" oh wow this is so awkward, "well you should get back to sleep" I said standing up.
"yeah" she said moving, I kissed her on the forehead "wait" she said grabbing my hand "whose room am I in?"
"mine" I said looking around
"oh, Gerard when was the last time you slept in a proper bed?"
"I don't know five nights ago, even longer"
"do you want to sleep, I could move over"
"nah, Im right" hang one you dickface she wants you to sleep with her! "actually I am kinda sick of sleeping in that chair"
"well get in here" she lifted up the sheet, I crawled over her, my back pressed up against the wall. I closed my eyes 'you are a dick head' the voice said.
It felt like I didn't dream that night, when I woke up I felt for Katherine, but she was gone my eyes snapped open, I sat up looking for her, something moved on the floor wait what. "why are you on the ground?" I asked her.
"well I fell off and im to sore and lazy to get back up" she laughed.
"do you feel ok?"
"no, I feel a bit off, as in a stupidity way"
"you are stupid" I got up and lifted her back up on to the bed "do you want me to get Ray to have a look at your burn?"
"yes please" she said, I walked out of the room.
"hey Toro go have a look at her burn" I said to him
"uhh, I'll be in soon, Mikey go have a look at her for me"
"'kay" Mikey got up from his place on the couch and walked into my room.
"oi" Ray said grabbing my arm " I saw her last night, shes too pale, ill have to give her a needle, the same one I gave you when you were shot"
"was she on the ground when you came in?"
"no, why was she on the ground?"
"she fell out of bed"
"oh… Did you hear what I said about the needle?"
"oh, yeah, you'll die trying to give it to her-"
"Well, someone scared her" I walked in to my room, still talking to Ray "she'll freak out"
"Well you're gonna have to do something to stop her, cause I need to give it to her"
"I'll try" we walked in Katherine and Mikey were laughing "what?" I asked
"I just scared the shit outta her"
"Katherine we have to do something and you won't like it" I said
"does it involve me getting hurt?"
"fuck my life" she poked out her bottom lip, then she saw what Ray had in his hand "ok now you can all get fucked"
"you have to have it, your pale and we've lost someone because they were shot and they didn't have this needle"
"I don't care-"
"hold her down" Ray said, I walked over to her and sat behind her head Frank sat on her knees and Mikey got her feet. I grabbed hold of her hands, she still struggled against our hold, wow she was strong, but she was so small. "this isn't gonna work" Ray said
"Yay!" she half yelled, oh damn it, what im about to do its gonna kill me. I leaned down a placed my lips on hers, I looked a Ray and watched him put the needle in her neck. I pulled away everybody looked at me. "what did I do?"


text:
Our group is defined by our commitment to our community principals. We strive to serve the community by discovering and developing better solutions for good health. We are a diverse, multi-cultural inclusive group where each member of our team is recognised, accepted and embraced. We respond to the needs of our community and encourage a culture of helping others to promote effective communication and improve the sharing of scientific knowledge.
The Cooper Group is contemplative. We continually examine and monitor progress via regular project and group meetings and annual performance reviews that provide the opportunity for everyone to communicate ideas, provide constructive feedback and advice and share positive experiences.
The Cooper Group is a safe and respectful workplace, where each member feels secure and valued. The University of Queensland's comprehensive workplace safety policies provide an effective framework for a healthy and safe work environment. Our individuals accept responsibility and accountability for safe and respectful working practise.
Matt Cooper
NHMRC Principal Research Fellow
Professor, Institute for Molecular Bioscience
Professor, School of Chemistry & Molecular Bioscience
Matt Cooper completed his PhD in Australia in 1995 and then spent 13 years in the UK, first at the University of Cambridge, then in start-ups and biotechnology companies. This involved innovation, fund raising, intellectual property generation, licensing, product development and commercialisation. He returned to Australia in 2009 as a NHMRC Australia Fellow. He has consulted with Private Equity Investment, Pharmaceutical, Biotechnology and Diagnostics companies, was Managing Director of Cambridge Medical Innovations (part of Alere Inc.) and CSO of Akubio Ltd. He is an inventor and driver of a several antibiotic drug discovery programmes with lead compounds pre-clinical. He is an expert in label-free technologies and their application to drug discovery and development with two books in this field. Full CV
Personal Assistant and Group Co-ordinator
Jan Pinder
Our Research Academics
Mark Blaskovich
Dr. Mark Blaskovich is a Senior Research Officer with extensive medicinal chemistry expertise and over 15 years of industrial drug development experience. As Chief Operating Officer at Mimetica (Australia), he managed the drug discovery and development program for melanocortin-5 receptor antagonists leading to a compound currently in Phase II human trials. In his role at CEPTYR (Seattle) he led a multidisciplinary team that developed a preclinical candidate for protein tyrosine phosphatase 1B, while at Molecumetics (Seattle) he headed a $2m/yr industrial collaboration on peptidomimetic compounds targeting proteases and GPCRs. Mark is currently project leader for the Wellcome Trust-funded program that is developing new antibiotics to treat drug resistant pathogens. He has published over thirty articles and patents, and is sole author of 'The Handbook on Syntheses of Amino Acids'.
Mark Butler
Dr Mark Butler has been a Senior Research Fellow at the Institute for Molecular Bioscience at the University of Queensland since September 2009. Prior to this, he worked in Singapore at MerLion Pharmaceuticals focusing on natural product chemistry and anti-bacterial drug development. Dr Butler is a recognised expert in natural product based drug discovery with over 15 years of experience in industry based collaborative research and has published over 62 papers and 4 patents. He was awarded the Matt Suffness (Young Investigator) Award by the American Society of Pharmacognosy in 2002.
Alysha Elliott
Dr Alysha Elliott is a Research Officer at the Institute for Molecular Bioscience and has a background in molecular and structural biology. She completed her BAppSc (Biochemistry) at the Queensland University of Technology before relocating to the Institute for Molecular Bioscience at the University of Queensland to complete her Honours year (First Class in Biochemistry). She has worked at the Institute for Molecular Bioscience as a research assistant for Professor Glenn King on spider toxins and antimicrobial peptides, before completing a PhD with Associate Professor Josh Mylne and Professor David Craik in the area of peptide based drug discovery from plants. Alysha is now part of the Cooper Group, where she is interested in the translation of research for antibacterial drug discovery.
Frank Fontaine
Dr Frank Fontaine is a bioassay officer at the Institute for Molecular Bioscience, UQ, since 2006. He has developed more than fourteen protein- and cell-based bioassays, using spectrophotofluorimetric and high-content imaging methods, in the following indications: CNS, cancer, diabetes, obesity, infection, inflammation, stem cells. He has completed a dozen of screening campaigns on marine extracts and pure compounds and has five years of industry experience in the field of antithrombotics for UQ spin off, Thrombostat Pty Ltd, and as a sub-contractor for European CIRD Galderma, Theramex, Zambon Pharmacia, ADME Bioanalyses and UCB Pharma Sector. He has 21 publications and is co-inventor on 1 patent.
Alejandra Gallardo-Godoy
Dr. Alejandra Gallardo-Godoy joined the Cooper Group in 2013 after more than a decade working and studying in the USA. Commencing her career as a PhD graduate at the University of Chile, she was soon awarded a Fulbright Fellowship to complete her degree at Purdue University in the USA. She continued post-doctoral studies at Purdue before venturing out to industry where she worked at Chemical Diversity Labs Inc. and Vertex Pharmaceuticals, as a member of the team that discovered the new Cystic Fibrosis drug KalydecoTM . With her experience working in industry, she returned to academia, joining the University of California-San Francisco (UCSF), as a Medicinal Chemist Specialist at the Small Molecule Discovery Center (SMDC). During her years as a medicinal chemist, she has predominantly focused on neurochemistry, GPCRs (Serotonin, Dopamine, Opioid, Muscarinic Receptors), neurodegenerative diseases like Creutzfeldt-Jakob Disease among others. She has published 10 papers and 2 patents.
Reena Halai
Dr. Reena Halai is a Research Officer at the Institute for Molecular Bioscience. She received her B.Sc degree in Biochemistry from Kings College London, during which she completed her placement year at GlaxoSmithKline in the Gene Expression and Protein Biochemistry Department. She then went onto work for the oncology department at UCB Pharmaceuticals before commencing her Ph.D. with Prof. D. Craik at the Institute for Molecular Bioscience. Reena has 6 papers in peer reviewed journals and is now part of the Cooper Group, where her current research interest is GPCR's.
Karl Hansford
Dr Karl Hansford is a Senior Research Officer within the Cooper group, and has eleven years' experience in synthetic organic, peptidomimetic, process and medicinal chemistry. After several academic appointments (IMB/UQ, University of Pittsburgh, University of Montreal), Karl moved into industry, spending several years in Montreal (Mimetogen Pharmaceuticals) and Melbourne (Boron Molecular) before returning to the IMB. He holds eleven peer reviewed publications and is inventor on two patents.
Xiao (Johnny) Huang
Dr. Johnny Huang is a research officer working at IMB since 2009. He gained his PhD in Molecular Cell Biology from the University of Tokyo in 2005. Dr. Huang has 4 years industry experience in cell culture and animal experiments. As a team leader, he successfully led the transgenic mouse team to develop a novel method for culturing mouse embryonic stem cells and generate over 200 lines of knockout/knockin mouse models. Dr. Huang is currently working on mode of action studies of novel antibiotics, as well as the research of drug-protein/membrane interactions. He is also interested in the application of SPR technology for protein, peptide and small molecule binding studies. Dr. Huang has 11 publications in peer reviewed journals.
Fredrik Lindahl
Dr Fredrik Lindahl completed his MSc in chemistry at Gothenburg University (Sweden) 2002 going on to complete his PhD at Natural Product Discovery/AstraZeneca/Griffith University in Brisbane in organic synthesis (total syntheses of natural products) under Prof. Ron Quinn (CEO of NPD). After some contract syntheses at Gothenburg University, he joined the David Fairlie group at The University of Queensland's Institute of Molecular Bioscience (IMB) in 2008 working as a Research Officer in medicinal chemistry targeting C5aR antagonists in the GPCR field. He later joined Prof. Jenny Martin's group (IMB, UQ) starting up a medicinal project targeting DsbA/B enzymes of the E-coli bacteria in the search of antibiotic therapeutics. He is currently working for Prof. Matthew Cooper (IMB, UQ) as a medicinal chemist and is part of a Wellcome Trust funded program developing new antibiotics for treatment of drug resistant pathogens. Fredrik has practiced many varieties of chemistries in the syntheses of small molecules SAR libraries to peptides – within the drug discovery field.
Sreeman Mamidyala
Dr. Sreeman Mamidyala is a Senior Research Officer who received BSc (1996, Osmania University) MSc (1998, Kakatiya University) and PhD (2004) from Indian Institute of Chemical technology, Hyderabad, India. He carried his postdoctoral research at Iowa State University with Professor Nicola Pohl and at Wayne State University with Professor Steven M Firestine. Then he joined as research associate with Professor M. G. Finn at Department of Chemistry, The Scripps Research Institute. His research interests include (i) click chemistry based drug discovery, (ii) in situ click chemistry (iii) carbohydrate chemistry, and (iv) synthetic methods.
Andrea Ranzoni
Dr. Andrea Ranzoni received his Msc in Physical Engineering from the Polytechnic of Milan before moving to The Netherlands to complete his PhD at Philips Research Laboratories in Eindhoven. He is currently a Research Officer at the Institute for Molecular Bioscience where his main interests are the development of novel nanotechnologies for in vitro diagnostics. He is author of four publications and inventor in six patents.
Avril Robertson
Dr Avril Robertson completed her PhD at the University of St Andrews, Scotland in 1999. Since that time she has gained more than 10 years industrial experience in medicinal chemistry and library synthesis. Prior to moving from the UK to join CDDD she worked as project manager and medicinal chemist at Cyclacel Ltd working on small molecule drug discovery in the field of oncology, Thermo Fisher Scientific on heterocyclic custom synthesis and Tripos Discovery Research managing contract research projects involving file enrichment, hit/lead generation and optimisation.
Daniel Watterson
Zyta Ziora
Dr Zyta Ziora has 10 years of academic postdoctoral experience in medicinal chemistry of antimalarial therapeutics, antibacterial agents and enzyme inhibitors (drug candidates against Alzheimer disease, Kyoto Pharmaceutical University, Japan). During her stay at the University of Montpellier (France) she was actively involved in the design and synthesis of the the bis-thiazolium derivatives which are currently in development for treating severe malaria in human clinical trials. She has also been trained in enzymology, microbiology and heamatology and has expertise in spectroscopy and Transmission Electron Microscopy. She has thirty two publications in peer-reviewed journals and is an author of a book chapter.
Johannes Zuegg
Dr. Johannes Zuegg completed his PhD (Chemistry, Molecular Modelling) at the Technical University of Graz (Austria) and spent one year as a PostDoc at the University of Graz (Austria) doing protein crystallography. After migrating to Australia he was working on Molecular Dynamics simulations for 2.5 years at the John Curtin School of Medical Research at the Australian National University (Canberra), before joining Alchemia Inc., a small Bio-Tech company in Brisbane, as Molecular Modeller in Drug Development for 2 years. After a one year interruption as Computer Research Specialists at Griffith University (Brisbane), he rejoined Alchemia Inc. as Head of IT and Molecular Modelling for another 5 years. He joined the IMB and the Cooper Group in May 2009 as a Senior Research Officer working across the different drug design and development projects.
Research Assistants
David Edwards
David Edwards is a Research Assistant with IMB. He has over 20 years experience of analytical chemistry working Pharmaceutical, Agrochemical and Flavour industries. Prior to his appointment at IMB he worked for 7 years with Pfizer UK within the structure elucidation group specialising in mass spectrometry, working with Prep LCMS, LCMSMS and ICPMS systems.
He gained his MSc. in Analytical Chemical Research at University College London comparing super critical fluid extraction with liquid/ liquid techniques of essential oils. These extracts were analysed by GC/MS.
Geraldine Kaeslin
Angela Kavanagh
Angela Kavanagh is a microbiologist from Universidad de Los Andes (Bogotá, Colombia). From 2006 to 2008, she was a Research Assistant at the Laboratory Microbial Ecology and Food at Universidad de Los Andes, researching in food pathogens and molecular biology. She then moved to the Institute for Molecular Bioscience, UQ as a Research Assistant in 2009 to work with Professor Rob Parton, testing caveosphere and siRNA or other small molecules to be incorporated into caveosphere. In 2011, she became a part of Matt's Cooper Group, to work on immunological and microbiology techniques and is currently working on the antibiotic project.
Ruby Pelingon
Ruby Pelingon is an Analytical Chemist/Research Assistant at the Institute for Molecular Bioscience at The University of Queensland responsible for purifying a library of novel synthetic compounds. Prior to this, she has worked at Interphil Laboratories, Inc., Philippines as a Quality Assurance Laboratory Analyst responsible for Analytical Method Validation. She received her BS in Chemistry from Silliman University, Philippines.
Soumya Ramu
Soumya Ramu is a Research Assistant at the Institute for Molecular Bioscience at The University of Queensland since November 2009. Graduated in Advanced Masters of Biotechnology from The University of Queensland, she has gained knowledge in techniques in molecular biology along with culturing and maintaining mammalian cell lines. Soumya also has expertise in immunological and microbiology techniques and is currently working on the antibiotic project.
PhD Students
Megha Bajaj
Megha Bajaj joined the Institute for Molecular Bioscience at The University of Queensland as a PhD student in July 2011. Prior to this, she received her Bachelor's degree in Microbiology (Honours Course) from University of Delhi followed by Masters in Bioinformatics. Subsequently she moved on to work with a life science R&D Company for two years, where she gained experience in microbial proteomics, structural biology and high performance computing. Her PhD focuses on the development of anti-infectives targeting microbial proteins to combat resistance against superbugs. Her main research interests are in microbiology and drug discovery.
Sisi Bi
Mu Cheng
Daniel Croker
Angie Jarrad
Angie commenced her PhD studies with the Cooper group at the Institute for Molecular Bioscience in 2012. Her PhD research is focused on developing novel antibiotics to fight anaerobic bacteria. Previously Angie completed her undergraduate studies at the University of Adelaide, graduating with a Bachelor of Science with First Class Honours and a Diploma in Languages (Italian) in 2011. Angie is an Australian Postgraduate Award recipient.
Sanjaya KC
Tran Thi Dat Nguyen
Wanida Phetsang
Zoe Schofield
Honours Student
Johan Deecke


text:
An Illinois 18-year-old male motorist recently entered into a plea agreement on an accusation of aggravated DUI. The charges grew out of an incident last December in which a car crash resulted in both him and a female passenger in another vehicle suffering injuries described as critical. In exchange for withdrawing his prior not guilty plea and admitting guilt to drunk driving, the teenager was given a sentence of felony probation. Had he been convicted at trial, he could have faced a much harsher sentence of a $25,000 fine and up to 12 years of incarceration.
The teenager had consumed alcohol at a house party before the crash. Police claimed that his blood-alcohol content tested at 0.145, which exceeds the 0.08 legal limit for motorists in Illinois.
At the time of the accident, his pickup truck allegedly went through a stop sign at an intersection, hitting the passenger side of a GMC Yukon vehicle. The teenager was flung from his truck as he was not wearing his seat belt at the time. His injuries left him paralyzed and in need of a wheelchair. The other vehicle rolled over and a female passenger suffered injuries which included a fractured ankle, fractured ribs, and a broken back.
Many teen motorists, in the course of their first early experimentations with alcohol, make the mistake of drinking and driving. In this instance, it led to an accident which has changed the teenager's life forever because of his injuries.
Such a momentary youthful indiscretion, however, does not have to completely ruin a young person's academic and professional future, given support and help from his parents and competent legal representation to minimize legal consequences.
Source: Quincy Herald-Whig, "Fowler teen pleads guilty to aggravated DUI charge from December accident," Don O'Brien, Oct. 18, 2012


text:
The manager of a Chicago CVS store is charged with a felony, accused of videotaping a store worker in the bathroom.
42-year-old Glennis Warburton, who ran a CVS in the Noble Square neighborhood, hid an iPhone in a store bathroom and then ordered a female worker to go change her shirt.
The woman called police saying she spotted the camera after Warburton sent her to the bathroom to change a second time.
205 N. Michigan AvenueGround FloorChicago, IL 60601Station Operator: (312) 565-5532Newsroom: (312)565-5533


text:
as low as $3.58
Notify me when this product is available:
"I want to see America free again! So, what do I do?" This is an often asked but rarely answered question, that is, until now... The answers to that question are...
View full product details →
Sign up to get the latest on sales, new releases and more …
© 2014 American Vision


text:
CATEGORY: Original Song
SPEECH BY: Adele Adkins and Paul Epworth
FILM: "Skyfall"
======BEGIN TRANSCRIPTION======Adele: Thank you so much. Thank you. This is amazing. I'd just like to thank Barbara Broccoli and Michael Wilson at Eon Productions. Sony Pictures, MGM, Paul Epworth, who, oh God, for believing in me all the time, and my man, I love you, baby. Paul: Thanks very much. I can't really believe I'm up here to receive this. Got to thank the Academy, of course. Allison Hooker at EMI, Sam Mendes. Barbara Broccoli, Michael Wilson, [Unintelligible] Who else? Jonathan Rose. I thank my wife, Danielle, my daughter, Vivienne… I'd like to thank Joe and Matt, who worked on this with us. They spent hours and hours, late nights in the studio with us to make this piece of music. And most of all I'd like to thank Adele for being the best person I've ever worked with. She's absolutely amazing. Adele: You're all amazing.
These transcripts may not be reproduced except as brief quotes used in conjunction with news reporting about the 85th Academy Awards®. All content Copyright 2012 Academy of Motion Picture Arts and Sciences. "Oscar®," "Oscars®," "Academy Awards®," "Academy Award®," "A.M.P.A.S.®" and "Oscar Night®" are the trademarks, and the ©Oscar® statuette is the registered design mark and copyrighted property, of the Academy of Motion Picture Arts and Sciences. Additional information regarding the "Terms & Conditions of Use" and "Legal Regulations for Using Intellectual Properties of the Academy" may be accessed online at http://www.oscars.org/legal/
Academy of Motion Picture Arts and Sciences
Communications Department
8949 Wilshire Boulevard
Beverly Hills, CA 90211-1972
Phone (310) 247-3090


text:
Dr. NTR University of Health Sciences has conducts the Under Graduate and Post Graduate Courses Examination in the month of April-May 2013. NTR University is going to announce the M.Ch DM MD BDS MBBS BAHM BHMS BSc 1st 2nd 3rd 4th 5th year exam results 2013 available at official website www.ntruhs.ap.nic.in soon. All those candidates are eagerly waiting for NTR university UG PG results 2013.
Large number of candidates who appear in all ug and pg courses exams. NTR university will soon going to publish the BDS MBBS BAHM BHMS BSc M.Ch DM MD semester wise exam results 2013. BPT results have been published earlier by the management. After examination attended candidates are eagerly waiting for NTR university results available very soon.
About NTR University:
The Government of Andhra Pradesh has the distinction of establishing the first University of Health Sciences fulfilling the recommendations of several committees. The University of Health Sciences started functioning at Vijayawada from 01-11-1986. After the death of its founder Sri N.T. Rama Rao the University was named after him as NTR University of Health Sciences.
How to Check NTR University Results 2013
1. Candidates Log on official website http://ntruhs.ap.nic.in
2. Than find the course result link and click
3. Now enter your registration no, Hall ticket no
4. than click Submit button
5. Finally display your results and tack a print out it.
NTR University Results 2013: Click Here
UG & PG NTR university Results at India Results: Click Here


text:
The lowly likes of us regular bloggers complaining in the support forums didn't have much of an effect. The only solution offered until now was logging in every day and manually changing the subject line to match your post—not fun.
But after Darren Rowse's open letter to FeedBurner two weeks ago on ProBlogger, things are finally changing. Today, the Google AdSense for Feeds blogs announces that "Happiness is more subject in your subject line." And it's true!
Now the interface under Publicize > Email Subscriptions > Email Branding offers this "hot tip":
Note the checkbox at the bottom: if you regularly have more than one post a day, the checkbox opens another menu with even more customization:
This subject line is only used if you have more than one item in your feed that day; otherwise, it reverts to the primary custom subject line.
What do you think? How fast will you be logging in to customize your subject line?


text: FASHION Fashion Galleries IWC Ingenieur AMG Jaeger-LeCoultre Master Perpetual Jaeger-LeCoultre Master Perpetual Rolex Datejust Rolex Datejust Richard Mille Tourbillon G-Sensor RM 036 Jean Todt Richard Mille Tourbillon G-Sensor RM 036 Jean Todt Baume & Mercier: The Clifton Series Baume & Mercier: The Clifton Series Coming Next OR 0 5 Back to Top ▲ IWC Ingenieur AMG IWC Ingenieur AMG IWC Ingenieur AMG Frank Geelen Share on Facebook Share on Twitter Shares Originally introduced in 1954, the Ingenieur was a no-nonsense, highly technical and sturdy timepiece. It was intended for use in the most severe conditions, capable of withstanding all the natural enemies of an accurate movement: magnetism, temperature, water, rough shocks and heavy wear. According to early advertisements, the Ingenieur was targeted at the "man who expects the impossible from his watch." Alongside the new genre of dive watches, such as the Rolex Submariner and the Blancpain Fifty Fathoms, which were also introduced in the '50's, the Ingenieur was the first sports watch on the market capable of withstanding all threats. Next Page Comments Load Comments More Like This Promoted Best of the Web Special Features


text:
The existence of Mysore State Anthem was bought to my notice by my uncle who in his primary school would sing the anthem every day. In 1881, the British decided to give back the state of Mysore to the Maharaja Chamaraja Wodeyar. Rao Bahadur … [Read more...]
Mysore Dasara Procession in Black and White
Wish you all a very happy Dasara! This Dasara procession photograph was part of my grandfather's collection. The photograph was probably taken between 1930 - 1935. … [Read more...]
Ayudha Puja
Ayudha Pooja is a celebration during Dasara. On this day the weapons used during war were worshiped in the palace. The common people worship machines, implements and vehicles at home, factories and offices. This is a grand celebration, and a time to … [Read more...]
Mysore Dasara Flower Show
Mysoreans love gardening and every house will have at least a Tulasi and Money plant growing in a pot. To encourage this wonderful hobby, a flower show on a grand scale is held by the Horticulture department during Dasara at Curzon park adjacent to … [Read more...]
Navaratri Golu
This is in continuation of the post "Traditional Dolls Arrangement". The custom of arranging dolls during Navaratri / Dasara is also followed in Tamil Nadu, Kerala and Andhra Pradesh. In these states it is known as Navaratri Golu or Kolu or Bommai … [Read more...]
Traditional Dolls Arrangement
Here are some Dolls that are handed over from generations to generations for arranging during Dasara. Traditional Dolls Arrangement includes Dolls in a marriage party complete with the bride / groom sitting in a car, the snake charmer, mythological … [Read more...]
Gombe Habba – Forest Arrangement
During Dasara, many households had the ritual of arranging dolls in the house for 9 days in Mysore region. This would bring out a lot of creativeness among the people. The preparations for the doll arrangement would start two weeks before the … [Read more...]
Pattada Gombe Alankara
Pattada Gombe in Kannada means, the royal dolls and Alankara means decoration. Many households have a custom of arranging dolls during Dasara festival in Mysore and distribute sweet and savories to children. This is also known as Kolu in Iyengar … [Read more...]
Dasara Procession and Torch Light Parade during the reign of the Wodeyars
Picture1 - Illuminated Mysore Palace Mysore Dasara procession during the Maharajah's rule used to be on a larger scale and grander. My grandparents and parents generation go on and on narrating the grandeur of the procession during "those days" … [Read more...]
Banni Tree
Notanical name of Banni Tree is The botanical name of this tree is Prosopis spicigera. In Sanskrit it is known as Shami Vruksha. Banni tree is found in dry and arid regions at low altitudes. This tree is widely grown in North West India and … [Read more...]


text:
A joint RCAI/JSI meeting "Development and Maintenance of the Immune System" was held July 26th and 27th at the Pacifco Yokohama Conference Center. Speakers were invited from Japan, Canada, Germany, the UK, and the US. Among the participants in this meeting were the students from the RCAI International Summer Program and some of the lecturers from that program also spoke here.
The first day's talks concerned the development of the immune system and mechanisms of lymphocyte selection. Dr. Toshio Suda (Keio University) focused on a very early developmental stage, the hematopoietic stem cell (HSC). He described the importance of the osteoblast, a cell normally thought of in terms of its role in bone formation, in the stem cell niche, a specialized microenvironment where the HSC reside in a quiescent state. Dr. Juan Carlos Zúñiga-Pflücker (University of Toronto, Canada) demonstrated an important role for Notch signaling in early T cell development. A Notch signal is required for expansion of αβ T cells and their survival at the single positive stage and PI3 Kinase and Akt are downstream effectors. By contrast, the γδ T cells can survive without Notch once they express a TCR. Dr. Al Singer (NIH, USA) described an interesting mouse model in which the TCR co-receptors CD4 and CD8 are deleted and signaling to the developing thymocytes occurs strictly through the TCR. Remarkably, if the major histocompatibility complex (MHC), which is normally what thymic T cells are selected on, is also inactivated, T cell development still takes place but the T cells in the periphery are not MHC restricted. Dr. Dianne Mathis (Harvard Medical School, USA) has been examining obesity and the role of low grade inflammation in adipose tissue in the development of type-2 diabetes. She found that regulatory T cells (Tregs) accumulate to an extraordinarily high fraction of CD4 T cells in the abdominal adipose tissue of mice as they age and that the cells have an unusual phenotype in comparison with Tregs in other tissues. Understanding the physiological role of these cells may provide new prophylactic treatment options for preventing diabetes.
Differentiation of lymphocyte subsets and the maintenance of a functional immune system were the focus of the second day's talks. Dr. Shinsuke Taki (Shinshu University) discussed basophils as a potential initial source of IL-4 for polarization of helper T cells toward the Th2 pathway. Analysis of gene targeted mice allowed him to define important regulatory molecules in IL-3 induced IL-4 production by basophils. Dr. Brigitta Stockinger (National Institute for Medical Research, UK) described physiological inducers for the development of Th17 cells, a recently described subset of helper T cells that produce IL-17. Zymosan, a yeast cell wall component and Mycobacterium tuberculosis were found to induce Th17 cells. She also noted that reported differences between human and mouse Th17 cells may not be real, but rather are an artifact of not starting with sufficiently pure naïve T cells when inducing human Th17 cell development in vitro. Dr. Marc Jenkins (University of Minnesota Medical School, USA) discussed strategies to enumerate polyclonal antigen-specific CD4 T cells in unimmunized mice in order to avoid the use of transferred TCR transgenic T cells to study the expansion and half life of these cells during an immune response. The frequency of peptide specific T cells is remarkably low and varies among different peptide epitopes, e.g. 220 versus 20 cells per mouse. The memory T cells generated after immunization decline slowly over time, with a half life of ~ 50 days for the one specificity studied in detail, and this may account for the loss of T cell immunity. Dr. Masato Tanaka (RCAI) has developed a system for tolerance induction in experimental autoimmune encephalomyelitis (EAE), a mouse model for human multiple sclerosis. Apoptotic cells expressing EAE self antigens are injected intravenously and protect the mouse against subsequent induction of EAE. Conditional knockout mice were used to identify the marginal zone macrophages in the spleen as important mediators of tolerance induction.
The next RCAI-JSI International Symposium on Immunology is tentatively scheduled for June of 2008.


text:
Turn autoplay off
Turn autoplay on
Please activate cookies in order to turn autoplay off
This content has been removed as our copyright has expired.
Your IP address will be logged
Christmas recipes: Nigella Lawson on desserts and baking
More from Word of Mouth blog on
More blogposts
Hugh Fearnley-Whittingstall on vegetarian options
How Hugh Fearnley-Whittingstall answered your questions about vegetarian Christmas dishes
Fergus Henderson talks turkey
Nigella Lawson's traditional Christmas cake
Fergus Henderson's whole roast suckling pig
Hugh Fearnley-Whittingstall's squash stuffed with leeks
Click here to join the discussion.
Last 24 hours


text:
Brian Doherty of Reason.com was able to visit the set of Atlas Shrugged, Part II — Either-Or, based on Ayn Rand's inspiring novel, during part of its ongoing 31 day shoot. After poor box office sales and being panned by critics, including our own Matthew Alexander (read his review), many wondered whether the second and third installments would ever be filmed. Encouraging DVD and VOD sales convinced the wealthy "rights"-holder, John Aglialoro, to persevere and a few new Randian investors to hop on board. I'm not sure whether the budget is the same, but in a risky move two things certainly aren't: the director and the entire cast.
Yes, you read that right. The director and the entire cast from the first film were not retained for the second and third installments. The irony is not lost on those of us who both recognize that so-called intellectual property is illegitimate and are painfully aware of how fiercely most followers of Ayn Rand cling to it that copyright appears to have played a major role in this potentially devastating turnover. As one of the producers, Harmon Kaslow, himself explains,
just from a practical standpoint when we set out to make Part I we had a ticking clock where if we didn't start production by a certain date John's interest in the rights could lapse. We didn't have the luxury at that moment to negotiate future options with the various cast members.
The need to rush to get the first movie filmed no doubt had a negative effect on its quality as well.
Kaslow tries to alleviate our doubts, "The message of Atlas is greater than any particular actor, so it's one of those pieces of literature that doesn't require in our view the interpretation by a singular actor." But, I'm sorry to say, while this is true, replacing the cast destroys the integrity of the three-part film as an artistic work — at least for me. It simply cannot be viewed as a seamless whole. There will be a jarring effect from the discontinuity in the transition from the first to the second installment.
The new director will be "John Putch (a TV veteran with many episodes of Scrubs and Cougar Town behind him)," so we can safely assume that, with a small budget and a tv director, Atlas Shrugged, Part II will feel more like a made-for-tv movie than a professional Hollywood film.
In light of Atlas Shrugged, Part I and these revelations, I cannot say I have much hope that the remaining installments will be good films.
Here's a brief run-down of some of the new cast:
- Jason Beghe, "most recently of Californication," as Hank Rearden;
- Esai Morales, "most recently seen as Caprica's Joseph Adama," as Francisco D'Anconia;
- Samantha Mathis, "perhaps most famously of Pump Up the Volume opposite Christian Slater," as Dagny Taggart.
On the plus side, a new screenwriter has been brought on board; apparently this Duncan Scott worked with Ayn Rand on a bootleg film adaptation of We the Living.
What's your opinion of the first film and the prospects going forward in light of these revelations? Tell us what you think in the comments.
Read the full story of Brian Doherty's experience on set.


text:
Full size version is only available to registered users
Please Register as a New User or Login as Existing User to gain full access to all the photos on this site. Registration is quick and completely free.
Unregistered users can only view full size photos that have been added this month.
Record shot of Curving Crack (VS4c)
© Chalky Cat, Mar 2008
Route: Curving Crack
Camera used: Pan Leica


text:
- Subscribe
- Magazine
- Contract Packaging
- Education
- Leaders in Packaging
- Calendar of Events
Alert: Packaging World now enhanced for the iPad and iPhone. Watch a quick video preview
Article | March 31, 1998
Gilbreth: Gilbreth: Timing screws on TE banding machine
Manufacturer CMS Gilbreth (Bensalem, PA) says installing new timing screws on the TRA line of TE banding equipment can increase throughput by 25%. CMS' tests indicate that 50 to 100 more containers/min can be banded when the new plastic screws are added, depending on lid or cap size.
The banders are suitable for OTC pharmaceutical vitamins dairy and food applications.
42 Best Package Designs
Sign up to receive timely updates from our editors and download this e-book consisting of our editors' picks of most notable package designs. Updated for 2014!


text: AltEnergyStocks.com has an article today entitled 10 Green Energy Gambles For 2009. If you're like most people, you have seen your traditional investments take a nosedive over the last year. If this is the case you might find this list of stocks that trade for between $.30 and $7.47 to be an interesting read. I would never suggest investing in a specific stock or even a general investing strategy, because I simply don't know enough about the subject. But I do suggest being familiar with as many different technologies as possible, and a lot of the companies mentioned in this article work with new and developing technologies.


text:
Stop anonymous access
October 2011, Products
The kit comes with a full set of accessories to get you started including 1,5 metre of network cable, a 2 G USB stick for downloading data and five RFID cards. The terminal is easy to install, with a user manual and training videos on CD and 60 minutes free support.
The TK110 provides employee-specific, shift-specific, area-specific access control with stored transactions for tracking who entered the area. It is suitable for retail stores, offices, server rooms, warehouses, hospitals, schools, restaurants or workshops.
For more information contact Uniclox Technologies, +27 (0)11 439 2000, email@example.com, www.uniclox.co.za
Their name
Further reading:


text:
Quebec Divorce Law: Grounds for Divorce
|< previous page
Quebec Divorce Law
The Divorce Act is a federal law made by the Parliament of Canada, administered equally across all provinces and territories. Only the superior court of each province has jurisdiction to deal with a Petition for Divorce under Quebec divorce law.
The Act stipulates the sole grounds for divorce as marital breakdown, and provides for three basic ways for proving it in Quebec divorce law:
For Quebec divorce law FAQs, click here.
Azran & Associes Avocats Inc.
|Add your listing
|Apartment Rentals
Add your listing
|Certified Divorce Financial Analyst
|Find a CDFA
|Add your listing
|Certified Public Accountants
|Add your listing
|Collaborative Practice
|Add your listing
|Add your listing
|Add your listing
|Canadian Legend Neil Young Files for Divorce After 36 Years of Marriage
Pamela Anderson and Rick Salomon to Divorce...Again
Judge Sides with Former Dodgers Owner Frank McCourt in Divorce Fees Dispute
|FREE Divorce Teleseminars
To Educate and Empower
Divorce People
Listen to podcasts of previous TeleSeminars.