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WordPress London October Meetup
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#wplnd October 2012 Meetup was held at Google Campus in the heart of East London's Tech City. The evening consisted of two talks.
Build a mobile theme to use with your existing WordPress site
The first talk was by Jack Barham, who worked throughout 2012 exclusively for Isobar Mobile, building mobile web apps for companies. He shared his mobile experience and knowledge he discovered working at Isobar Mobile. Having a vast collection of different mobile phones at his disposal to test with he developed his experience into a basic mobile template. The talk was about how to use this template and wrap it into a plug that can be dropped into a WordPress website. The presentation covered all the aspects of how he approach a mobile web build project, such as: Concepts, Wireframes, Prep your Current WP site for mobile, Design, Screen Resolutions, Development (Mobile CSS Reset, WP Code, Mobile jQuery, etc), Bug Testing.
View the presentation on Slideshare.
Getting started with WordPress web apps
The second talk was by Rachel McCollin, all the way from Birmingham. With her talk she focused and used the examples of her recently published book WordPress Mobile Web Development. The talk mainly focused on using WordPress to build web apps, starting off by discussing the difference between a web app and a responsive site. And how you can use your theme to create a web app from the same database as your main WordPress website.
Pub Time
As a freelance WordPress developer, going to the pub afterwards is another highlight of the evening. I always enjoy a bit of WordPress geek-off and bouncing off ideas with other WordPress lovers!
WordPress London September Meetup
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#wplnd September 2012 Meetup was held at Skillsmatter, and once again an enjoyable collaboration of WordPress freelancers, workers and users. The evening consisted of two talks.
Practical tips for Captioning YouTube Videos
Web developer and accessibility guru, Graham Armfield show how to incorporate captions in your YouTube video. Something which would help those with hearing impairments and those viewing the videos in noisy or office environments. The presentation was a thorough step by step of all the settings and different ways to incorporate the captions. He also showed other tools available to use for videos and captions.
View the full presentation podcast on the Skillsmatter website.
Rapid WordPress theme development
The next talk was by Jonny Allbut, Head of Digital at Tanc Design. He is also the creator and lead developer on the Wonderflux theme framework, presents at various web development conferences and sits on the WPUK committee that coordinates the annual WordCamp in the UK.
He first start off with his typical 'tool box' and why. Followed by the different theme types available – WordPress theme frameworks, parent, child and starter themes. With his experience with Wonderflux, he shares how a framework can help you to build more flexible, sustainable custom themes. He also shared a few important lessons learned: Do things the WordPress way. Use the core filters, hooks and functions. Keep up with trunk development/Trac. Documentation.
View the full presentation podcast on the Skillsmatter website.
Ask the Experts
Turned out that there was a bit of time left for questions and answers from the attendees. I find this part exciting to hear different people's view points and ideas about different issues. And also how eager people are to help.
#dConstruct 2012 Conference
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dConstruct 2012, held every first weekend in September in Brighton, had their 8th conference this year. It is presented by Clearleft, a user experience design consultancy based in Brighton, UK. This year was my first year to attend this conference. The theme of this year was 'Playing with the Future'.
dConstruct 2012 Workshop/s
There were two workshops available to attend on Wednesday, 5 September 2012 and two Thursday, 6 September 2012.
I attended Jonathan Snook's workshop, Scalable and Modular Architecture for CSS, in short SMACSS (pronounced "smacks"). Jonathan came up with this new term and approach from his experience working with the team at Yahoo! Mail. By dividing your CSS into 5 sections: Base, Layout, Module, State and Theme you can write CSS in re-usable chunks of code. Also keeping in mind using classes for layout rather than ids will illuminate having to overwrite loads of CSS. Applying these approaches in the right way will result in smaller CSS file/s. He also motivate using child selectors and preprosessors. He prefers to rather add the class of the reusable CSS to an element in the HTML, than selecting HTML elements in a CSS file. There was also a talk about prototyping tools that can help with rapid development, especially on big projects.
Besides the free new SMACSS book, I definitely took a new approach to CSS with me.
dConstruct 2012 Conference
To summarise the conference, it is what it says on the website's home page:
dConstruct 2012 is a gathering of smart and entertaining people poking at the intersection of technology and culture.
The conference being moulded over the years to topics covering web technologies, design, culture and society, the theme 'Playing with the Future' just did that. With a collection of excellent speakers it is hard for me to say which one stood out.
Ben Hammersley started off the day with his talk The Flower, the Field, and the Stack. With all the beautiful things in the world and how the community in the world have taken code that was not too good looking twenty years ago and made it into something beautiful. In every layer we work in, from fiber optic cables, to binary, to unicode, to XML, HTML and CSS, to grids and design patterns… all of these thing in every level, we can find a beauty and elegance that has been hard fought for.
Jenn Lukas's vibrant talk, Cure for the Common Code, was about breaking down the barriers of web publishing by embracing the rise of code education. She teaches HTML and CSS for GirlDevelopIt. It was an inspirational talk of how we all would like to learn something new, but just need that little extra push on the backside. With GirlDevelopIt, she helped create an opportunity, for that push, for woman that would like to learn code.
Scott Jenson has always been evolved in mobile, realising how it is unlocking important things. His talk Beyond Mobile: Making Sense of a Post-PC World start off with a question, if we take new technology and explore it or do we turn around and apply it to what we did yesterday? He became aware of 3 trends. The first one he calls App Glut. Users become the bottle neck and managing the apps being made. The second trend – value must be bigger than pain and the equality that can be between value and pain. The third trend – smart devices tend to be on the simple side. It has applications that offload a significant amount functionality. Moving forward, how can these trends be applied to make apps that can work across all of our devices.
With Ariel Waldman's talk, The Hacker's Guide to the Galaxy, I could feel her absolute passion and enthusiasm for science. Her talk was an interesting tour of our universe. From black holes and dark matter to exoplanets and the search for extraterrestrial intelligence. She was showing examples of peoples creations that can get anyone excited to start making things.
Seb Lee-Delisle (laterally) lighten up the dome after lunch with his talk Pixels, Play and People. He brings people together with technology. Everyone received a glowstick at the beginning of the talk. The highlight of his talk was using a motion camera and everyone was able to make digital fireworks! By using the motion camera he also divided the crowd in a left side and right side to play a form of Astroids together!
Jason Scott, a computer historian, besides for his well known cat is also famous for archiving everything. He most certainly looked very comfortable on the stage with his talk The Save Button Ruined Everything: Backing Up Our Digital Heritage, a good recap of how we have been saving, how saving data has changed over the years and how we save things today. Thus, when MySpace go down and you are looking for your 'stuff', be sure to contact this guy…
Tom Armitage is a game designer at Hide & Seek. His work is inspired by the toys his dad made out of wood when he was a kid. He sees all his work as toys and decided to have a talk titled, Making Friends: On Toys and Toymaking. He has had quite a bit of fun with Twitter too. For example, he was auto generating descriptions of chocolate from chocolate boxes. He also created a Twitter account for Towerbrige, by taking the times of when the bridge opens and linking it to the internet via Twitter. It has become useful to cab drivers too.
James Burke, a science historian, an author and television presenter ended of the day with his speech titled, Admiral Shovel and the Toilet Roll. He looks at the past (there is no where else to look…), because the future don't exist and the future is the past, just with a few extra bits attached… Through the whole talk, an interesting point that stood out was his view on Nano Technology. What Nano Technology is going to do socially and when. He points out there is a possibility, in 40 years that everyone would be able to have their own Nano factory your own shed/garage. And also talking about the possibilities of this and the effect this could have on the world.
Missed out on #dConstruct 2012 Conference? Be sure to catch up at huffduffer.com.
WordPress London Meetup #6
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WordPress London Meetup is still a baby, with the first Meetup that took place 9 June 2011. Besides the success in attendance it also had a change in name. Starting out as London WordPress Meetup it is now officially WordPress London Meetup group. Clearly a much needed Meetup group for London locals, I am happy to have attended 3 of them so far. The 6th meetup took place Thursday, 20 October 2011—Publishing with WordPress, hosted by the Telegraph.
How the Telegraph use WordPress
First up was Paul Gibbs, core developer of BuddyPress and web developer at Daily Telegraph. That's right, Telegraph Blogs is powered by WordPress and MyTelegraph is possibly the largest BuddyPress website in the UK. Paul shared some of the plugins they use for the respective websites.
Telegraph Blog plugins:
- Yoast Breadcrumbs (more information at yoast.com)
- WordPress SEO (also by Yoast, more information here)
- Widget Logic (lets you control on which pages widgets appear)
- Unfiltered HTML (gives blog Administrators and Editors the ability to post whatever HTML they want)
- TMG Open Graph (based on the Open Graph plugin for adding additional metadata to webpages to identify them as "social objects"— modified further by the development team at the Telegraph)
- Blackbird Pie (add embedded HTML representations of actual tweets in your blog posts)
MyTelegraph BuddyPress plugins:
- BuddyPress (a social network in a box—more information at BuddyPress.org)
- BuddyPress Groupblog (extends the group functionality by enabling each group to have a single blog associated with it)
- SSO (single sign-on) Integration
- WP Report Posts (puts a link to each posts and pages to report that particluar post or page)
- Yoast Breadcrumbs (more information at yoast.com)
- Sitewide Tags (creates a new blog on your server for all the posts on your site, much like http://wordpress.com/tags/)
- Blackbird Pie (add embedded HTML representations of actual tweets in your blog posts)
WordPress and Web Accessibility: Why it's Important
Graham Armfield was on for the second talk of the evening. His presentation covered some issues that disabled and elderly users experience with websites. He shared valuable techniques to improve accessibility and why it makes business sense.
Graham's presentation, WordPress and Web Accessibility, can be viewed at SlideShare.
Upcoming WordPress London Meetups
Thursday, 17 November 2011: WordPress SEO and Custom Post Types
Wednesday, 7 December 2011: Christmas social!
#WordCampUK 2011 in Portsmouth
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I attended my first WordCamp this year. It was also a first for me to Portsmouth! Below I talk about my personal running order. Read about more sessions at WordCamp UK 2011 Portsmouth here.
Saturday, 16 July 2011
Kicking the day off early, Saturday 16 July 2011, registering took place from 9:30 and I received my official WordCampUK 2011 t-shirt. Tony Scott started off the day at 10:00 with an introduction that followed onto sharing stickers that leads to more pleasand introdutions! I topic that all WordPress fanatics love followed after that: How are you pushing your WordPress install to the limit? I am even more impressed now…
Legacy to Latest by Kieran O'Shea
Kieran's talk was about how plug-ins have an influence when upgrading WordPress.
For example before upgrading, consider:
- Modified default themes
- Deprecated functions
- Phantom features (what is phantom features?)
Plugins can break an upgrade, or be broken by an upgrade, deprecated functions.
A solution is Custom Posts. Legacy plugins may over-use tables. Post framework makes code simpler. Migration then is simplified.
Visit Kieran O'Shea's dedicated website for WordCamp UK 2011 Portsmouth. Where you can find loads off goodies, eg.
Slides: PDF of Presentation Deck
Code: Zip File of Code
WordPress and Mobile by Rachel McCollin
Rachel discussed 4 ways how you can turn your website into a mobile version.
- Mobile themes
- WordPress plugins
- Media Queries
- Bespoke mobile websites (or just make an app version)
Advanced SEO with WordPress by Nick Garner
Nick told a lovely SEO story of The White, black and illegal Hat in WordPress SEO. This story shared so much SEO information in a friendly way. Check out his presentation.
Some of his favourite plugins to use are:
- wp-supercache plugin
- yoost plugin
- wordpress categorie radion buttons
- seo-image plugin
WOW Plugins 2011 by Michael Kimb Jones
First few favourite plugins mentioned:
- Yoost SEO plugins
- Google XML Sitemaps (switch something off)
- WP Super Chache (W3 Total Cache – best to use?)
- WP e-Commerce
- Jigoshop (New)
- MarketPress (WP multi)
Other few mentioned randomly:
- Jetpack (loads if functionality in one plugin – X6)
- Members Only
- WordPress Beta Tester
- Widget Logic (conditional loads for Widgets) Dynamic Widgets
- Extended Page List
Final list of 10:
- CMS Tree Page View (extremely handy if you have A LOT pages!)
- CollabPress (for project management)
- Contact Form 7
- Easy Table Creator
- Custom Post Type UI
- EG-Attachments (for external document files)
- Front end editor
- Regenerate Thumbnails
- Widgets on Pages (just check it out, it's really cool)
- WordPress reset (good plugin for a test site)
After this it was the wrap of the day that lead to dinner and a lot of free drinks at the Dolphin. After this… what happens at WordCamp stays at WordCamp…
Sunday, 17 July 2011
After a well needed full English breakfast the second (and last) day of WordCamp started off with a quick introduction by Tony Scott again.
Ten Proven Steps to the Top of Google (SEO) by Mark Adams
Another SEO session with more handy tips..
- Keyword Research - with Panda update, quality of website very important
- Basics – Homepage links – SEO friendly URLs, keywords in page name and URL
- Page structure – one keyword per page, maybe 2, images – title tag, images – file name
- Copywriting – at least 250 words per page
- (missed this slide :-/)
- Blog – Important to talk about topic website is about, become expert in the topic (authoritive)
- Social media and online PR
- Link building – get links from sites with related topic
- (missed this slide :-/)
- Call to action
Some handy tools:
How to content manage everything by Robert O'Rourke
A few points taken….
- custom menus
- drop down
Post types - register post type using available of arrays
- things that are neither posts or pages
- Forums – bbPress plugin
- Properties
- Events
- Static bloks or 'boilerplate'
- Landing pages
- Other applications
Themergency is a handy tool to easily create post types. After registering it appears in admin bar.
Use Metabox API to add/remove or reorder meta boxes:
Also visit http://codex.wordpress.org/Settings_API
Site doctors: Let the experts help you! by Jonny Allbut (Jonnya), Nick Garner
A Questions & Answers session with informative discussions and tips going around.
Sadly all good things do come to an end. Well sort of… I noticed emails with loads of ideas going around already for 2012. And if you can not wait till then, the WordPress community do spread their wings to numerous Meetups and other smaller conferences.
First London Blog Club Meetup #LondonBlogClub
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The London Bloggers Meetup Group first came to my attention. It is a rather big meetup group. I continued my search though, to find a brand new meetup group for bloggers in London: London Blog Club. Murray Newlands and Marko Saric (running successful blogs of their own) go to the London Bloggers Meetup Group. After the last event they decided to start their own meetup group.
Tuesday, 2 February 2010 was their new group's first meetup at The Pitcher & Piano in Holborn. The Central Line train transported me from west to east quicker than I anticipated. I was officially the first attendee at the very first meetup of London Blog Club. Not long after me, more bloggers started to arrive and I enjoyed finding out what part of the bloggersphere they reside.
Where do I find myself in the bloggersphere?
Chatting to other bloggers about their blogs and blog goals (achieved and to achieve) opened up a discussion to myself about my own blog. My website originally was (as mentioned before) to showcase my work. The time then arrived to do a bit of the other. At school, writing and grammar was not one of my strong points. I looked for a bit of help from the book: On Writing Well by William Zinsser. It is (hopefully) helping me to not miss the message and waffle to many long sentences. And if I do, to revert to my core objective and what I am trying to say.
After the meetup when I went through my RSS feed I came across this article: You Got Design Right – Now 'Write' Design! – a blog post, from a writer to a designer (how great is that…). In the blog post Dave Thackeray explains his view point of the similarities between the design and writing processes. It made me feel more at ease with the future of my blog (and adamant now not to fail at Project 52).
The joy and reason for me for blogging is sharing my design experiences, other designs I like, event and travel experiences. I also have a few topics up for discussion. As time go by I am sure a few more will come to mind.
Back to London Blog Club Meetup
The first meetup already got my mind to start working. I would not be surprised to see a rapid growth of the London Blog Club. RSVP 'Yes' for the next meetup Tuesday, 2 March 2010 and I will see you there!


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Pop star Justin Timberlake was awarded a permanent restraining order today in L.A., according to TMZ.
The judge initially said Timberlake had to show up in person and testify. The singer/actor's attorney argued that Timberlake was under contractual obligation to be on set for a movie.
Eventually, the team submitted a declaration on his behalf and later Karen McNeil was ordered to stay 100 yards away from him, his home and car.

subset: commoncrawl


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Author Comments
Sir Clock exhibits his fine, non-explosive flash cartoon for Clock Day 2006! Surely his new flick will be the greatest yet.
Authors Note: I really did make this in the hours of Clock Day :P
**I do respond to all reviews.
Rated 5 / 5 stars June 15, 2010
The best of 2006 Clock Day
Easily one of the best Clock Day flashes I have ever seen because there was simply so much work put into it. You can just watch these things and notice the little stuff in the background. No matter the content, you can simply never go wrong with having that "Requiem For A Dream" music in it. The ending was also great, as both parts of the flash itself were brilliant. It was also funny and random to have CorpseGrinderClock at the end as well. There is so much crazy stuff going on and so many Clocks in it, it is a perfect addition to the Clock collection!
Rated 5 / 5 stars June 21, 2009
Very random, and very funny.
It was hilarious. I laughed a lot. I liked the part where the computer gets an error and explodes, sending SirClock flying out the window.
Rated 5 / 5 stars March 21, 2009
I fell off my chair just watching this.
This was so freaking funny but when i fell down on my chair i hurt my wrist and i missed the rest of it.
Rated 5 / 5 stars February 18, 2009
Nice flash
It's funny and the animation is perfectly drawn
And you lied that you said that you respond to all reviews and you didn't respond any!
Well,who cares
Atleast the flash is awesome :)
Rated 5 / 5 stars January 26, 2009
Very Funny!
I liked the part with him killing bitclock with a Deustch Mp5k
(and yes I am a gun nut!)


text: Profile Topics Started Replies Created Favorites Forum Replies Created Viewing 1 post (of 1 total) Author Posts May 30, 2013 at 1:39 pm in reply to: Please contribute and translate Enfold #114934 nohowonParticipant Hi! I'd like to translate the Enfold file into Spanish, but when I download de enfold.pot from mediafire it's only 729 bytes (already tried it a couple of times). It seems too small a file. Can I use the enfold.pot found in the the last update of the theme? Thanx! Author Posts Viewing 1 post (of 1 total)


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Shortly after midnight as Mitt Romney conceded the presidential election to Barack Obama, Ole Miss students in Oxford, Miss., gathered to protest Mr. Obama's re-election.
A disturbance ensued after angry students assembled near the center of the university in protest.
Officials say racial slurs were tossed in a crowd that grew to about 400 students after rumors spread that a riot had broken out.
Two arrests were reported but university officials say there were no injuries or property damage.
Images on Twitter showed the brief unrest, presumably from the results of the presidential election.
The disturbance lasted until around 1 a.m. before students disbanded.
A university spokesman says the gathering began after midnight with 30 to 40 students protesting Obama's re-election but quickly grew. Rumors exploded on Twitter after student journalists posted a video calling the gathering a riot.
Chancellor Dan Jones on Wednesday condemned the disturbance, saying most students and faculty are "ashamed" of the actions of a few.
He said inaccurate comments and photos posted on social media sites don't match police observations.
In the wake of the protest, the University of Mississippi released a statement. The full text of the release reads:
Statement from the University of Mississippi
OXFORD, Miss. - University police were notified by students shortly before midnight Tuesday that Twitter chatter was indicating students were gathering near the student union to protest the results of the election. The officers found 30-40 students gathered in front of the union, and over the next 20 minutes the gathering had grown to more than 400 students, many of whom were chanting political slogans. The crowd was ordered to disperse by university police, and after about 25 minutes students had returned to residence halls. About 100 students gathered again at one hall, and university police dispersed the group and made two arrests for disorderly conduct, including one for public intoxication and one for failure to comply with police orders.
"While we are grateful that there were no injuries and there was no property damage, we are very disappointed in those students who took a very immature and uncivil approach to expressing their views about the election," said University of Mississippi Chancellor Dan Jones. "The gathering seems to have been fueled by social media, and the conversation should have stayed there."
"Unfortunately, early news reports quoted social media comments that were inaccurate. Too, some photographs published in social media portrayed events that police did not observe on campus. Nevertheless, the reports of uncivil language and shouted racial epithets appear to be accurate and are universally condemned by the university, student leaders and the vast majority of students who are more representative of our university creed."
Jones said, "Parents are being notified that it's a normal day on campus and that one of America's safest campuses is safe again this morning, though all of us are ashamed of the few students who have negatively affected the reputations of each of us and of our university. We are initiating a thorough review of this incident to determine the facts and any follow-up actions that may be necessary."
Jones said, "We are reminding our students of relevant statements within the university creed: The University of Mississippi is a community of learning dedicated to nurturing excellence in intellectual inquiry and personal character in an open and diverse environment. As a voluntary member of this community: I believe in respect for the dignity of each person. I believe in fairness and civility. I believe in personal and professional integrity."
Associated Press reporter Holbrook Mohr om Jackson, Miss., contributed to this report.
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Hi! My name is Allyssah and I love reading. Now I'm using wattpad as a way to share my ideas and storied I think of. I do not upload regularly at all, but I hope you all like what I have! I love romance, adventure, fantasy, sci-fi, etc.
Living With the Crazy, the Weird, and the Love
Help Me Lovely
Before I FallWhen Jennie finds out that she has been diagnosed with Leukemia, her life is put into perspective. From being a successful athlete, on her way to a scholarship, to sitting tr... read more


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We all know that Green Bay Packers legendary Coach Vince Lombardi decided to step down from his coaching responsibilities after the 1967 season and following his Super Bowl II victory over the Oakland Raiders.
What we don't know is that his father was really ticked about his decision to do so.
In today's release from the Green Bay Packers Hall of Fame (one of my favorite Packers sites, by the way), we find out that on this day in Packers history Lombardi told the story at the Dapper Dan Dinner in Pittsburgh. "For what have you given up coaching," the senior Lombardi asked his son. Lombardi said at the dinner that he told his father what he had told everyone – that the job of coach and general manager had gotten too big to handle.
Isn't it funny how history has a way of repeating itself? How many coaches over the past decade have experienced similar issues? Those with Packers ties – Mike Holmgren and Mike Sherman come quickly to mind – suffered the same issues.
But back in the day, Lombardi saw the writing on the wall and decided to give up coaching and stay on with the Packers as general manager. That lasted one season. When he realized he loved coaching too much and wanted to get back into it he left Green Bay and headed out to Washington, D.C., to coach the Redskins. He was there a single season, turning that franchise from a losing proposition into a winner in that single year. We can only guess what he may have accomplished in Washington had he not died before the start of his second season there.
"Dad wanted me to stay on forever as coach of the Packers," Lombardi told those at the dinner. "He thinks that coaching the Packers is the greatest position going, and I agree with him."
We also heard those same words from Mike Holmgren before he left Green Bay for a big contract and more power in Seattle.
The elder Lombardi must have been a very wise man.


text: Loom Dataset Management for Hadoop. Loom automatically tracks and manages dataset lineage for Hadoop. Auto Scan dynamically profiles datasets, collecting valuable metadata. Lab bench provides data scientists with a lightweight intergace for finding transforming and analyzing data in Hadoop and Hive. All Loom metadata and functionality is exposed through RESTful APIs. Integration with R boosts productivity for data scientists. (PRNewsFoto/Revelytix)


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Following the launch of the Losing Ground project on coastal erosion in South Louisiana, journalists from The Lens and ProPublica are holding a panel discussion Tuesday about how they reported the story. Continue reading »
Carrollton-Claiborne intersection lane closures planned overnight for water-leak repairs, officials say
The busy intersection of South Claiborne and South Carrollton avenues will be reduced to one traffic lane in several directions starting at 7 p.m. tonight (Wednesday, Sept. 3) for repairs to a leaking water main, authorities said. Continue reading »
Entergy to turn off the power Wednesday near Jefferson Avenue drainage work
Drainage work to close two blocks of Prytania for a year
Chestnut Street crossover at Napoleon Avenue to close for 30 days
The Chestnut Street crossover intersection at Napoleon Avenue closed this week and will remain inaccessible to drivers for 30 days, officials announced. Continue reading »
Three former Louisiana governors agree: Lawsuit against oil companies should proceed
Although former Louisiana governors Buddy Roemer, Kathleen Blanco and Edwin Edwards have a number of political differences, all three agreed Wednesday night that no state officials — neither the legislature nor the current governor — should interfere with the local levee board's lawsuit against oil companies. Continue reading »
Uptown drainage project to close lanes of St. Charles during summer construction near streetcar tracks
Contractors working on the massive Uptown drainage projects along Jefferson, Napoleon and soon Louisiana avenues plan to fit their work crossing the St. Charles Avenue streetcar tracks from June to August of this year, and during those months, St. Charles Avenue will be reduced to two lanes of traffic, according to a report by Monica Hernandez of our partners at WWL-TV.
Letter to the editor: Side streets, school traffic need more attention during Uptown drainage projects
By Clark Thompson
If you live in Uptown New Orleans, you've probably had the misfortune of driving on Octavia street in the past few months. The US Army Corp's SELA project effectively closes Jefferson Avenue, and ends up sending lots of traffic onto Octavia, and the wear and tear of additional use is destroying the street. And the street is destroying cars, but that's already been covered. Continue reading »
Drainage work will rope off Napoleon Avenue neutral ground during Mardi Gras 2015, officials say
Construction on the massive new drainage canal under Napoleon Avenue will soon begin moving toward the river, and sections of the neutral ground will likely be inaccessible to parade-goers during Mardi Gras next year, New Orleans officials said Tuesday night. Continue reading »
Construction equipment to be removed from Jefferson Avenue near Magazine for Mardi Gras, but four blocks of Prytania to close for a year soon afterward
The Corps giveth and the Corps taketh away: The large structures blocking Jefferson Avenue near Magazine Street are in the process of being moved in time for Mardi Gras parades to make their usual turns around that corner, but soon afterward a four-block stretch of Prytania will close for about a year, officials said Wednesday. Continue reading »
Prytania closed at Arabella for three months for drainage work
The intersection of Prytania and Arabella streets will be closed until the end of March as part of construction on the new underground drainage system along Jefferson Avenue, New Orleans officials said. Continue reading »
Water pressure will drop most of Tuesday morning (Jan. 7) along the upper end of Prytania Street for work related to the installation of the new Jefferson Avenue drainage canal, officials said. Continue reading »
Drainage work to close Jefferson Avenue at Clara Street for two months
The preparation for installation of a new drainage canal under the Jefferson Avenue neutral ground will require closing both lanes of the road at Clara Street for two months starting on Monday, New Orleans officials announced. Continue reading »
Tree trimming, removal of neutral ground beginning on Jefferson Avenue
Workers preparing Jefferson Avenue for installation of a major new drainage canal will spend the next several weeks before Christmas trimming trees and removing the neutral ground, New Orleans officials said. Continue reading »
Amid government shutdown, Corps of Engineers skips Jefferson Avenue drainage canal meeting
Although the U.S. Army Corps of Engineers is responsible for overseeing the construction of four major drainage canals around Uptown New Orleans, the federal-government shutdown caused the agency to miss a planned public meeting Thursday about the beginning of the latest phase on Jefferson Avenue. Continue reading »
Block of Jefferson between Constance and Laurel to close for two months
The block of Jefferson Avenue between Constance and Laurel streets will close for two months as part of the installation of the new underground drainage canal, New Orleans officials said. Continue reading »
Same contractor to extend new drainage canal down Napoleon through 2016
Boh Brothers Construction Co., the contractor currently installing a drainage canal on Napoleon Avenue between Claiborne Avenue and Carondelet Street, won the contract last week for the final phase of extending that canal down to Constance Street, authorities said. Continue reading »
Remaining Jefferson Avenue trees to be cleared from Claiborne to Danneel
Major changes to Uptown drainage systems represent citywide water-management proposal
Rainfall should be diverted out of Uptown via the Mississippi River instead of carrying it all the way to Lake Pontchartrain, and major drainage ditches like the Monticello Canal should be expanded into interior floodplains and water-storage features, according to two recommendations that illustrate how New Orleans should be better managing its water instead of just pumping it away.
The Water Management Strategy presented by architect David Waggonner to a standing-room only crowd Thursday evening at Xavier University is a regional plan for making more efficient use of rainfall, slowing it down and storing it in natural canals to reduce the sinking of the land that contributes to flooding. The recommendations in the Uptown area are only a small part of the plan, but they illustrate some of its key elements and some of its challenges.
"We're proposing this is a new era for water management," Waggonner said. "It's not just about flood protection any more. It's really about quality and sustainability." Continue reading »
A broken, collapsing section of the 900 block of Webster has caused passing drivers to bottom out their cars or to take dangerous, last-minute moves to avoid it for a year or more, but it has finally been repaired after a project that required replacing underground pipes for most of the block, reports Bill Capo and our partners at WWL-TV.


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Sometime soon after SXSW, I'm going to get my very first garden up and running. I already have a general idea of where I want it and what I'm going to plant.
I think I'm going to build a raised bed garden because the ground is pretty hard in my yard. If I owned the house, I'd probably take the time to try to bring the soil back to life, but I'm not sure if that makes sense since Betsy and I might not be here forever.
Expect an update or two as I venture into my first gardening experiment!
After I repaired and seasoned my cast iron skillet, I want to make something in it. You pick!
And a few links!
America's Best New Sandwiches Take 2 – Another list of some awesome sandwiches being served throughout the US these days. If you happen to live in a one of these lucky cities, I hope you find the time to hunt down one of these! I'll be on the look out for the Chacarero next weekend! (@ Endless Simmer)
Bulgogi Cheesesteak Sandwich – Speaking of sandwiches… my friend Dan posted this on Tablespoon.com yesterday. An awesome twist on both bulgogi and cheesesteak. There's no way I'd turn one of these down. (@ Tablespoon)
Marinated Fresh Anchovies – I absolutely love anchovies. It's one of my favorite appetizers. While I don't mind the canned variety, freshly marinated ones are just out of this world. I'm not sure I can get my hands on any fresh ones, but if you're lucky enough to have them around, do this. (@ Sippity Sup)
Artichokes and Cheesy Grits – Artichokes are notorious for being a big pain in the butt to prepare so if you're going to do it, make sure you have a great application for them. This looks about as good as it get in my book! (@ No Recipes)
Have a great weekend everyone!
Photo by LollyKnit.


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Here's how life works.
For those of you currently experiencing some sliver of success or are in an upswing professionally:
It ebbs and it flows.
At the time this flier was printed, Defeater was a new band. This is Hell was well-established. Gravemaker had some hype. And PS Eliot was starting to become a more-than-local act. As End of a Year, we had toured through Birmingham a few times and had some fans there.
Now, Defeater is significantly bigger than any of the bands on the bill. This Is Hell has carved out a space for themselves, but has not grown. PS Eliot reached some notoriety before breaking up, with members now reaching a larger audience as Waxahatchee. Gravemaker cooled down. And, as Self Defense Family, we've still got a few fans in Birmingham.
I stumbled across this flier and it got me thinking about how some people see the world. They see trajectory and assume there'll be uniform gravity. They don't account for wind resistance or whatever other physics shit slows an object down.


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I know I'm in the middle of a series as well as a blog sabbatical, and those things are still happening. But. Some things are just worth the breaking point in time, you know? So. Two posts in one day, and a minor interruption for a big! huge! announcement.
A few months ago, I hosted the first Story101 retreat off in the hill country of Texas. You may remember my post about breaking in the marching boots—about the women marching together not in order to fight something but to fight for each other. This is what I want to be about, because we all feed the river.
A few weeks later, I got an email.
It was one that shook me to my core. One that sent a resounding yes! to the deepest places. It spoke of a dream to gather, equip and unleash the women of this generation to live out their purpose.
(I get chills still when I type it.)
Let me tell you: this dream is crazy. But it's the crazy ones that capture my heart, and it's the impossible ones that point me to Jesus. And the leadership behind this vision is bold, energetic and lion hearted.
And we're gathering in February.
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
A fresh, deep, honest space for a new generation of women to wrestle with the essential question: IF God is real… THEN what? This 2-day conference brings women together and wrestles out how to live out the calling God has placed on our lives. //Austin, TX Feb. 7–8 2014
I know some of you may be shying away because it's another conference. For women. You know this track. You've done it before. Can I just whisper in your ear for a second? This is different. I mean, I'm a tattooed coffee addict when I'm not drinking French 74's who leans toward colorful language more often than not and I'm so excited about this. I'm serious. For the past few months I've been so cynical about everything that relates to church-y stuff and when this came into my inbox, my heart felt at rest.
This is about friends gathering together, sharing their stories, and trying to dig deep in what it means to follow this purpose burning within our souls. Mark the date on your calendar and make sure to sign up for updates?
I'd love to meet you and hear what makes this heart of yours race with vision.
Oh and get this. Today, when everything went live, you want to know what they posted on their Facebook page?
This is bigger than any of us. It's time to break in those marching boots. Join us?
Want to hear more from others? Check out these posts for more info --
Jennie Allen
Lindsey Nobles
Jen Hatmaker
Sarah Markley
Lore Ferguson
Emily Freeman
Sarah Bessey
Raechel Myers
Bianca Olthoff
Jessica Honneger
Melissa Greene


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Optimal Income Taxation: An Exemple with a U-Shaped Pattern of Optimal Marginal Tax Rates
AbstractUsing the Mirrlees optimal income tax model with quasi-linear preferences, the paper examines conditions for marginal tax rates to be rising at high income levels and declining in an interval containing the modal skill. It examines conditions for the marginal tax rate to be higher at a low skill level than at the high skill level with the same density--an argument only holding for skill levels above a cutoff where resources of a worker are marginally of the same value as resources of the government. Data on earnings rates are presented. Copyright 1998 by American Economic Association.
(This abstract was borrowed from another version of this item.)
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Bibliographic InfoPaper provided by Massachusetts Institute of Technology (MIT), Department of Economics in its series Working papers with number 94-14.
Length: 8 pages
Date of creation: 1994
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Handle: RePEc:mit:worpap:94-14
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Postal: MASSACHUSETTS INSTITUTE OF TECHNOLOGY (MIT), DEPARTMENT OF ECONOMICS, 50 MEMORIAL DRIVE CAMBRIDGE MASSACHUSETTS 02142 USA
Related researchKeywords: income ; tax policy;
Other versions of this item:
- Diamond, Peter A, 1998. "Optimal Income Taxation: An Example with a U-Shaped Pattern of Optimal Marginal Tax Rates," American Economic Review, American Economic Association, vol. 88(1), pages 83-95, March.
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Support GroupSave as Favorite
Cancer and Careers hosts a monthly support group for people working during or after their cancer treatment in New York City. The Elizabeth Jerrett Support Group has been running since 2007 and has developed into a close-knit group of survivors who provide each other with wisdom and laughter year after year.
The group is at full capacity and is currently closed. If you would like to be added to the wait list please contact Kelsey Fenton at email@example.com.
If you are looking to join a support group outside of New York City please check out our Resource Directory or contact email@example.com and we'd be happy to help.


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Webber admits other teams 'interested' for 2013
Red Bull Formula One driver Mark Webber (R) of Australia holds his trophy next to Prince Albert II (C) and Princess Charlene (L) of Monaco after winning the Monaco F1 Grand Prix May 27, 2012.
The Australian struggled alongside reigning world champion Sebastian Vettel last year, but two weeks ago in Monaco he returned to the top step of the podium.
Webber, 35, is now equal on points with his younger teammate, and just three points from Fernando Alonso's title lead.
"It was a long season last year," he told French Canadian newspaper La Presse in Montreal.
"But this year is very similar to 2010 for me: I started strong and I fought for the championship until the end," said Webber.
It is a timely boost of Webber's form amid rumours of talks with Ferrari, as his Red Bull contract runs out at the end of the season.
"This is a results-based industry," said Webber. "This season is going well for me.
"To see that some other teams are interested in my services is nice," he admitted in Montreal.
"But I am very, very happy at Red Bull. We've done a lot together.
"It's just rumours for now, but it makes me a bit more comfortable," said Webber. "That I am having good results makes the other teams realise that I am doing better than an average job," he insisted.
Source: GMM


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Wilson Co.
July 2, 2012 5:52pm
According to the Massachusetts Governor, that state seems to be doing great with Romney care. 98% of people covered, cost driven down, its not a budget buster, and everyone for the most part seems happy with it.
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Miss Universe, Leila Lopes of Angola is scheduled to be in Guyana next month for five days, from September 12th - 16th.
Leila five-day visit to Guyana is to assist with various aspects of the local leg of the Miss Guyana pageant, this according Khalsan PR Inc, the official spokesperson for the Miss Guyana Universe Pageant.
Miss Lopes is scheduled to meet with President Donald Ramotar, she will also visit the Kaieteur Falls, the Georgetown Public Hospital, University of Guyana, and the Palms
The Miss Guyana Universe will be held on September 15th at the Princess Hotel and Casino, where fourteen delegates will vie for this year's crown.
The 61st Miss Universe Pageant will in December 2012, a venue and date have yet to be announced.


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Moderated by Tom Sabulis
Here's a seasonal column from an Atlanta pastor who also writes for the Higher Ground blog at www.highergroundgroup.org.
By Joseph L. Roberts Jr.
Today, we desperately need to connect the values taught by all our religious institutions — Christian, Jewish and Muslim — with our daily practice as citizens.
A few Sundays ago, after worship at Ebenezer Baptist Church, we stopped at a gas station. My wife and I remained in the car while our son filled the tank. A middle-aged man walked by. He wore three pairs of worn-out pants, each pair slipping on his thin frame, showing soiled undergarments. He seemed depressed and desolate, but he didn't approach us to ask for assistance. He probably wrote us off as people coming from church, who had already forgotten the challenge heard at worship.
He searched two large trash bins nearby, looking for any discarded food. He quickly wolfed down anything that was edible. He searched again, for something to drink. When he found a few soda cans, he swallowed the last drops. Then he pulled up his pants a third time, and summoned the nerve to enter the food market at the station to ask them if they had anything edible – something that they would discard anyways. They answered him rudely and negatively, and then forced him out of the store. In a moment, he was gone, but we didn't know where he went. Even worse, we didn't go looking for him. We could have helped him, but we didn't try to even try to find him. There was no connection.
We had just left a glorious worship service down the street, but in linking the experiences inside the sanctuary with those outside at the nearby gas station, we failed miserably. There was no connection.
He expected nothing from us and, sadly, we offered him nothing. We went back to business as usual.
Will we ever connect?
Jorge Mario Bergoglio, an obscure Catholic priest, was elevated a few years ago to Archbishop of Buenos Aires. While serving, he was criticized for not protecting liberal clergy during the country's "Dirty War." Yet, look what he did after. He tripled the number of so-called slum priests and opened new chapels for the poor in devastated areas of the city.
He repaired soccer fields in these neighborhoods and he stayed in touch with the chapels in the poorest sections. He was not given to pomp and circumstance. He took public transportation. He recently contacted a drug rehabilitation center celebrating its fifth anniversary, and left this message: "Don't let them steal your hope."
That's connection.
You may know this archbishop as the newly elected Pope of the Roman Catholic Church. No wonder he chose the name Francis, Pope Francis. He reminds us of St. Francis Assisi, that beautiful humble priest who was not ashamed to do the work of the gospel, to wash the feet of beggars, to give the hungry something to eat and, most important, to look into the eyes of the needy and see God.
If we can ever do this, we will make the connection between our creeds and our deeds beyond the sacred walls and corner gas stations. We will begin to find the true unity of all of the people in God's world. In this love connection lies our hope.
Rev. Joseph L. Roberts, Jr. is pastor emeritus of Ebenezer Baptist Church in Atlanta.
December 21st, 2013
11:21 am
Has anyone but me entertained the tho't that it might not be mere coincidence that two of the most powerful and influential offices on the planet have passed to men who are trying to turn Christianity away from the Pharisaism and parasitic hypocrisy that has subsumed wealthy, conservative, Christians (they hope). And on my best day I'm an agnostic.
I do sympathize with your dilemma in helping the man at the gas station. It has become immensely apparent to me that this, the self-proclaimed most Christian nation on Earth, has profoundly failed to institute the infrastructure, knowledge base, and even the will to fulfill even the most rudimentary decrees outlined by the New Testament when it comes to the poor and downtrodden. The wherewithal to reach out to that man is, sadly, rarely to be found within the boundaries of these United States.
I am a former Christian and I know that taking care not just of each other, but especially the poor and disadvantaged, should have always been considered a huge priority within the mission of the Christian Church. It has not. Yet Christians complain that government is stealing from them in order to do for the poor what they themselves, according to their faith, should have always been doing.
For this reason I believe that any grouping of Christians, whether it be an individual congregation or a denomination, should voluntarily forgo its tax exempt status with the IRS if it doesn't spend something north of 50% of its income and personpower on behalf of the poor.
Thank you for your honesty, as far as it went.


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Need a Rugged Tablet? Panasonic Has Two New Toughpads
Panasonic has launched two new Toughpad tablets at the 2013 International CES show, one running Android and the other based on Windows 8 Pro. The FZ-G1 (pictured above) runs Windows 8 Pro and is quite a powerful beast, with a 10-inch, 1,920x1,200 pixel display, a 1.9GHz Intel i5 processor, up to 8GB of RAM, a 128GB SSD, while the microSD slot is an option. On the connectivity front, it packs USB 3.0 and 2.0 ports, wired LAN, Bluetooth, Wi-Fi and -- optionally -- a Verizon LTE or 3G chip.


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Despite only having a single game under her belt, we all still love Beyond Good & Evil's Jade
About: Mary Goodden
My favourite games have plots, and love exploring the ways games tell stories. I've been "exploring" this on God is a Geek since January 2011, taking the odd break to play Super Smash TV, drink fine ale and rock out.I compare video games to romance novels, vent fury at celebrity voiceovers and nurse a severe Tomb Raider complex over at my blog, Well-Rendered.
Recent Articles by Mary Goodden
Character Select: GLaDOS
In Portal, you beat the boss on your own terms. GLaDOS is not alone in being the object of a satisfying boss fight that provides a fitting narrative and mechanical climax to a well-paced linear game. But she is unique in the sense that in killing her, you're destroying the very heart of a gameworld, that's kept you, the player, captive as much as it has the protagonist Chell.
Character Select: Lara Croft
Game: Tomb Raider (2013)
Species: HumanIn her own words: "I don't think I'm that kind of Croft." This is the first episode of Character Select not to contain spoilers about the character it's based on. However, it does contain Ghost in the Shell spoilers, so if you've yet to see the 1995 movie,...
Hyperdimension Neptunia Victory Review
Mary gets to grips with the latest offering from the JRPG table, Hyperdimension Neptunia Victory – but is it worth investing the hours to complete, or is it a case of style over substance?
Character Select: Clementine
Like all good horror stories, The Walking Dead taps into a primal human fear. It's a zombie story, and like many zombie stories, it doesn't exploit our fear of death so much as our fear that all our earthly struggles are futile.
Interview: The SWERY Files – Part One
During a recent world tour for Deadly Premonition: Director's Cut, we had a chance to sit down with SWERY himself, to talk about all things Deadly Premonition.
Character Select: Wakka
On this week's edition of Character Select, Mary takes a look at Wakka from Square Enix's Final Fantasy X
Character Select: Francis "York" Morgan
In this week's edition of Character Select, Mary takes a look at Deadly Premonition's Francis "York" Morgan. Read the full article at GodisaGeek.com.
Character Select: Katherine Marlowe
In this week's edition of Character Select, Mary takes a close look at Uncharted's Katherine Marlowe. Read the full article at GodisaGeek.com.
Character Select: Classic Lara Croft
In this edition of Character Select GodisaGeek.com's Mary Goodden takes a look at the Classic Lara Croft. Read the full article at GodisaGeek.com.
Mugen Souls Review
Mugen Souls describes itself as an "over-the-top, overwhelming RPG!" (exclamation mark included), and it's probably worth noting that neither of those things are necessarily good. Read the entire review at GodisaGeek.com.
Character Select: Frank Pritchard
As long as games have sought to tell stories, they have used characters like Frank Pritchard to help them do so. Read the full article at GodisaGeek.com.
Tales of Graces f Review
If you're looking for a deep, strategic battle system or an accessible introduction to a genre that can sometimes seem impenetrable, Tales of Graces f is a solid choice.
Character Select: Bayonetta
In this edition of Character Select, Mary takes a look at the infamous, yet ever-so amazing, Bayonetta. Read the full article at GodisaGeek.com.
Character Select: Mordin Solus
In the first of our ongoing Character Select articles, Mary takes a look at Mass Effect 2′s Mordin Solus. Read the full article at GodisaGeek.com.


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Times Square of the 1980s: A Short DocumentaryJuxtapoz // Wednesday, 28 Jul 2010
Siegel captures a pre-Disneyfied Times Square at night two decades past. As seen from a teenager's point of view, the short film includes voice-over interviews with local teenagers. A great blast from the past and a reminder of the importance of an era where so many of our current influential artists gained traction.


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£1 Entry or FREE for Members, Platinum, Platinum Extra and Returners Card holders
Full film listing can be seen at Flix Student Cinema
Scrat's nutty pursuit of the cursed acorn, which he's been after since the dawn of time, has world-changing consequences - a continental cataclysm that triggers the greatest adventure of all for Manny, Diego and Sid. In the wake of these upheavals, Sid reunites with his cantankerous Granny, and the herd encounters a ragtag menagerie of seafaring pirates determined to stop them from returning home.
Charity Number 1146696. © Loughborough SU 2010. Powered by Membership Solutions Ltd


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The needle has landed
Leanna Weaver receives an inoculation for the H1N1 virus from RN Patrician Johnston, during an event sponsored by the Kitsap County Health District Wednesday at the Silverdale United Methodist Church. Some 5,000 inoculations were made available to at-risk residents on short notice, with shots available regardless of need beginning Monday.
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Colorectal Cancer Health Center
Well-Rounded Prevention May Lower Colon Cancer Risk
Sigmoidoscopy only examines the rectum and left side of the large bowel, or colon, whereas colonoscopy examines the entire large bowel. "The distinction is important," Antman tells WebMD, "because benign and cancerous tumors are distributed evenly throughout the colon."
Fortunately, nature also offers protection against colon cancer. "Aspirin and [nonsteroidal anti-inflammatory drugs] block the enzyme that activates cancer cells, but there are natural sources that do the same thing," says Mitchell Gaynor, MD, director of medical oncology and integrated medicine at the Strang Cancer Prevention Center in New York City. "The herb rosemary, the spice turmeric, the skin of red grapes, and green tea all have similar effects as the [nonsteroidal anti-inflammatory drug] Celebrex."
Janne was supported with a fellowship grant from the Friends of Dana-Farber Cancer Institute.


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A 200 ft-by-85 ft sheet of ice is the stage for hockey players all around the world. Whether it was just learning to skate or playing their way through college and junior leagues, the surface where they perfect their craft has been a constant.
In the NHL, teams are encouraged by their fans when they play at home. Conversely, teams are told to ignore the home crowd when they play on the road. That is something that really does not need to be said out loud. It is common sense.
There are teams that flat out play better on the road. In the case of the 2010-11 Boston Bruins, they posted a better road record during that season (24-12-5). Those numbers gave the Bruins the third best road record in the Eastern Conference. When they played their home games at the TD Garden that season, they were ranked sixth in the conference with a 22-13-6 home record.
Fast forward to this season. The Bruins posses an impressive 17-7-2 record at home, which is the second best home record in the conference only behind the Washington Capitals. As the season comes down the stretch, it would not hurt to keep improving on this record to make their home ice a tough place to play come postseason.
The Bruins will have to endure another six-game road trip coming up in the month of February. This sounds like a daunting task for some teams, but not for the Black and Gold. Right around this time last season they went on a six-game road trip and walked out with no extra tallies in the loss column. They finished a perfect 6-0-0.
What it comes down to is that when one discusses home ice advantage, there is a point where you need to throw stats and records away. In the minds of professionals, or at least it should be, a sheet of ice is a sheet of ice
Everyone remembered when an injured Nathan Horton dumped some "Boston ice" on the sheet at Rogers Arena in Vancouver two hours before Game 7 of the Stanley Cup Finals. He was later quoted as saying, "Don't worry boys. It's our ice now."
How much of it was psychological? It still really was not their ice despite bringing their own shavings of ice from back home. The Bruins did have great success at home during the Finals, outscoring the Canucks 17-3. At the end of the day, they still had to win the Stanley Cup away from their home ice.
Having the depth and balance to be a successful team both at home and on the road is vital. The question is how many teams can pull it off? The Bruins are sure to be included in that conversation.


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Grandma Ida and #MadMen's race problem: #SlateTV
RT @TheRoot247: Our #RaceManners columnist @jdesmondharris is taking your race-related etiquette questions on #Reddit at 1pm. Go >>
Here's who would win in a race between the Enterprise and the Millennium Falcon--WATCH: #geekery
Marathon Champ "Boston Billy" on Why the Bombing Won't Hurt the Race:
RT @cnnbrk: 2 blasts happened at 2:45 p.m., about 1 hour after the first of the race's nearly 27,000 runners crossed finish line....
This is what a #MadMen episode that actually addressed race would look like:
Why silly speculation about the 2016 presidential race can do some real good:
How do they decide who gets the death penalty? Number of victims, money, public opinion, and (perhaps) race:
Struggling with a race-based dilemma? @TheRoot247's @JDesmondHarris will answer q's in new column, #RaceManners!
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Optimization of Human Embryonic Stem Cell Derivation Techniques and Production/Distribution of GMP-Grade Lines
Optimization of Human Embryonic Stem Cell Derivation Techniques and Production/Distribution of GMP-Grade Lines
Funding Type:
New Cell Lines
Grant Number:
Award Value:
Stem Cell Use:
Embryonic Stem Cell
Cell Line Generation:
Embryonic Stem Cell
Public Abstract:
Statement of Benefit to California:
Progress Report:
Year 1The goal of our work is to derive new human embryonic stem cell lines for distribution to stem cell researchers. During the past year, we produced 11 new lines that we think will be valuable tools for all phases of work toward regenerative medicine therapies including discovery, translational, and eventually, early clinical applications. Along the way, this work is teaching us a great deal about early embryonic development and the specialized processes that distinguish critical steps in humans as compared to animal models. What is the source of the embryos for our derivations? We use embryos that are left over after the conclusion of fertility treatments such as in vitro fertilization. In all cases, we obtain written informed consent from both individuals whose reproductive material was used to make the embryos. Additionally, researchers in our group have no direct contact with the couple making the donation. This process is handled by the University of California San Francisco Gamete and Embryo Bank. It is important to note that the embryos that we use would otherwise be destroyed, a point that is sometimes overlooked. What are the methods that we used to derive these new human embryonic stem cell lines? The work that was carried out in the last 12 months employed two techniques. One line was derived using standard methods that have been employed for many years to produce stem cell lines in many species including humans. In general, early-stage human embryos are thawed and grown for a few days in the laboratory until a cluster of tightly packed cells, termed human embryonic stem cell colonies, emerges. Stem cell researchers can spot these special cells based on their appearance under a microscope. The advantage of this technique is that it is a straightforward method for reliably producing human embryonic stem cell lines. The disadvantage is that the progenitors we seek are exposed for extended periods of time to other cell types in the culture, which could impact their genetic makeup and, subsequently, their developmental potential. Accordingly, we used a different method to derive the other ten embryonic stem cell lines that we produced during this grant period. The overall approach was to grow human embryos donated from a single couple until they contained 8-12 cells. Then we used specialized equipment to remove single cells from five sibling embryos. Each cell was allowed to develop in isolation. Four cells from one embryo gave rise to cell lines as did three cells from another embryo. The remaining three embryos each produced one cell line. This unique set of lines will enable stem cell researchers to understand the role that genetics plays in specifying the basic characteristics of human embryonic stem cells as compared to environmental signals that are inevitably transmitted as the lines are propagated in the laboratory. We are also interested in the concept that single cells that are extracted from very early stage embryos, which have not received signals from other cell types that are found at later stages of development, are more likely to be truly pluripotent as evidenced by their genetic and molecular signatures. Why do we need additional human embryonic stem cell lines? Many of the most commonly used stem cells have been in culture for many years. This creates several problems. First, the extended length of time that the most commonly studied human embryonic stem cells have been in culture means that they are likely to have accumulated errors that are the result of mistakes made during the self-renewal process. This is all the more likely to occur because we do not yet understand the optimal conditions for growing the cells in the laboratory. Accordingly, ongoing studies in many groups, including our own, are designed to improve the environment for maintaining human embryonic stem cells, which we think will improve the fidelity with which they make carbon copies of themselves. Second, nearly all of the existing lines have been exposed to animal products, which raises the possibility that they could contain disease vectors. For this reason, the Food and Drug Administration makes it difficult (but not impossible) for cell-based therapies that are used in clinical applications to be approved once they have experienced these types of exposures. Therefore, all of our derivation work has employed solely human reagents, which we think will streamline their approval for use in clinical applications.
Year 2Our goal is to derive human embryonic stem cell (hESC) lines using new and improved methods. During the first two years of this project, we produced eleven new hESC lines. One line was derived from an intact six day-old human embryo that consists of 50-100 cells. Nearly all existing hESCs were derived using this approach. The other ten lines were derived using a new method that we helped to pioneer. This technique entails removing a single cell from an earlier stage human embryo that is comprised of 8 cells. Under the right laboratory conditions the cell makes copies of itself, a process that produces an hESC line. The advantage of this method over the more conventional approach is that we know when the founding cell was removed and where it came from. Additionally, the founder is isolated from the signals of other embryonic cells, which could erase aspects of the stem cell state. Finally, we derived multiple lines from individual embryos so subsets of the lines have the same genetic makeup, which will allow scientists to study the influence of the environment on basic stem cell properties. During the current grant period, we registered the cells with the California Institute for Regenerative Medicine (CIRM). The process entailed submitting the paperwork that includes proof that the couples who donated embryos to this project gave informed consent. We also described the conditions we used for deriving and propagating these cells. Overall our methods emphasized the use of human materials, which avoids exposure to animal products that could be contaminated with infectious agents. These cells are now available for use by CIRM investigators and other scientists who have nonfederal research funds. This year the National Institutes of Health (NIH) initiated a parallel process for registering hESCs. The goal was to greatly expand the number of hESC lines that investigators would be allowed to use in experiments that were paid for by federal dollars. To make the cells we derived with CIRM funds more widely available, we attempted to register our cells with the NIH, which required submitting paperwork that was very similar to the documentation CIRM required. The line derived by conventional methods was quickly approved. However, the lines that were derived from earlier stage embryos have not yet advanced through the approval process. There were two problems. The first was that the Federal government defined an hESC line as coming from an embryo that consists of 50-100 cells. Our lines that came from 8-cell human embryos did not meet this criterion. Accordingly, the Government notified the public of the intent to change this definition. A comment period, which has now ended, ensued. However, the lines were still not approved. It then became apparent that a lawsuit contesting the use of federal funds for hESC research had been reinstated. Thus, ten of our lines are still listed as pending on the Federal Registry and we (and other investigators) cannot apply for (or use) Federal dollars to study them. Our scientific progress included establishing a bank of the hESCs that we derived. The purpose of a bank is to stockpile cells at a particular stage for future use. The need for banks acknowledges that the basic properties of hESCs can change as a function of the amount of time they are propagated in the laboratory. To bank our cells, we expanded them to the point where we were able to store approximately a thousand vials that contained a million cells each. All were frozen after they had self-replicated only twelve times, which reduces the chance that untoward changes occurred. Thus, we are now in a position to distribute our cells to other investigators who want to use them in their work. Importantly, the size of the bank ensures that we and other investigators can return to this source to get additional vials of the same cells that had been cultured for the same length of time. In additional experiments, we compared the properties of the ten lines that were derived from early embryos with conventional hESCs. In one series of experiments, we took a global approach in which we analyzed the fundamental properties of the cells. The results yielded data that suggested that the new lines had unique properties. We also found that lines that were derived from different embryos were more similar than lines that came from the same embryo, possible evidence that differentiation begins much earlier during the beginning stages of human development than was previously thought. Finally, we asked whether the fundamental differences we observed between our new lines and conventional cells were mirrored at a functional level. These experiments are still in progress, but we have interesting evidence that supports this possibility. For instance, we found lines that came from the same 8-cell stage human embryo differed in their ability to make the kinds of neurons that are envisioned as cell-based therapies for Parkinson's Disease.
Year 3The goal of our project is to improve methods for generating and banking human embryonic stem cells (hESCs), which are derived from human embryos. Since the federal government does not support work with human embryos, this research would be impossible without the funds we have received from the California Institute for Regenerative Medicine (CIRM). Additionally, our project has benefited greatly from CIRM Facilities Grants, which provided us with this specialized nonfederal laboratories that we need for experiments that employ human embryos. Our project has three Specific Aims. The first is to derive hESC lines from intact human embryos that have been grown in the laboratory for approximately five days. This is the method that has been used to derive the overwhelming majority of the existing hESCs. In these experiments, cells that are destined to become hESCs emerge over several days. Thus, the precise origin and timing are unknown. Some investigators think that this element of randomness contributes to the significant differences that have been noted among hESCs. To date, we used this approach to derive one line, UCSF-4, which was approved by CIRM. Thus, other grantees of this agency can work with these cells. Since they were generated using standard techniques, UCSF-4 was approved by the National Institutes of Health. Therefore, researchers who have federal funding can also work with these cells. The second Specific Aim proposed a new approach for deriving hESCs. We wanted to make lines from single cells that were removed from embryos at very precise stages of development. To date we have focused on the eight-cell stage, embryos that have been grown for three days in the laboratory. In these experiments, we used five embryos that were donated by a single couple. In all, we established 10 lines from individual cells. In other words, we derived multiple lines from the same embryo. Thus, this collection is unique because some of the lines are genetically identical and all have a high degree of genetic relatedness. This special property is important to stem cell biologists as we are trying to understand the changes that happened over time as the cells are maintained in the laboratory. Additionally, we have evidence that hESCs that are derived from embryos that have been grown for three rather than five days are more plastic in that they are better able to form the precursors of a broader range of cell types that comprise the human body. Like UCSF-4, the ten lines that were derived from single cells removed from embryos were registered by CIRM. They were also submitted to the National Institutes of Health along with UCSF-4. However, we were notified that they do not fit the federal definition of an hESC line, which specifies derivation from an embryo that has been grown for five rather than three days in the laboratory. Accordingly, these lines are still pending approval, which will require changing the federal definition. Therefore, it is uncertain if they will ever be registered, a process that allows federally-funded scientists to work with hESC lines. Our third Specific Aim was to derive a hESC line using Good Manufacturing Processes (GMP) as specified by the Food and Drug Administration. These guidelines have been established to make sure that cell-based therapies are safe for patients. For example, they are designed to prevent the spread of infectious agents that can contaminate animal cells to humans. One obstacle to producing GMP-grade cells is that hESC derivation requires a carpet of another cell type, which is thought to "feed" embryos the substances that are required for generation of hESC colonies. Thus these "feeder" cells must also be produced using GMP methods. To avoid this laborious step, we have developed methods for the "feeder-free" culture of hESCs. We plan to use the same approach to derive hESCs from intact embryos. To date, with CIRM support, we have derived 11 hESC lines. Therefore, distributing these cells has become a significant part of this project. It is interesting to note that these lines are being used for a wide variety of experiments. For example, UCSF-4 is being studied by a Consortium that has been formed to understand how chemical modifications to genes control their expression. This is a particularly interesting question because the programs that specify the initial stages of human development are very poorly understood. The other groups who are using our cells are focusing on a wide range of normal and disease processes. Several investigators are studying the mechanisms by which cells of the brain and spinal cord develop. One group hopes to use them as a therapeutic strategy for patients with amyotrophic lateral sclerosis or Lou Gehrig's disease. Other investigators are studying formation of cardiac muscle, pancreatic beta cells and the liver. Finally, our lines are also being used for training purposes in our CIRM Shared Research and Teaching Facility.
Year 4The goal of our work has been to derive new human embryonic stem cells lines from embryos that are donated by couples at the completion of in vitro fertilization cycles. Many laboratories including our own, derived lines using the method that was originally described. Embryos that were grown for 5-6 days in the laboratory were transferred to a lawn of cells that supported the eventual outgrowth of human embryonic stem cells. Using standard methods to perform derivations, we were struck by ways in which we thought this method could be improved. For example, the cells of the embryo that are destined to form the placenta die. Therefore, it seemed likely that the products they release could have untoward influences on the stem cells. In addition, we observed the formation of morphologically distinct cell types before tightly packed "islands" of stem cells finally emerged in their midst. We speculated that these extraneous cells could also have undue effects. Finally, we examined, at very high magnification, embryos that were grown for 5-6 days. Although the cells that give rise to the offspring are supposed to be nearly equivalent at this stage, we saw that they no longer look alike. We took this as possible evidence of specialization, meaning they might already be starting down the road toward becoming one of the cell types of the body. Therefore, we began to consider new approaches for producing human embryonic stem cells. In particular, we wanted to derive lines from single cells of earlier stage embryos that might be less specified and more plastic in terms of their ability to differentiate into progeny that are difficult to obtain from conventional human embryonic stem cell lines. In preliminary experiments, we worked with other scientists from the biotechnology sector to develop methods for deriving lines from single cells (termed blastomeres) that were removed from embryos that were grown for approximately 3 days in the laboratory. From the work of other scientists we knew that around this time the intrinsic genetic programs of the egg fade and those of the embryo start to unfold. Therefore, it was quite possible that human embryonic stem cell lines derived at this stage would be less specified than their counterparts, which were established by conventional methods. However, our initial work showed that this new approach was not very efficient. Although a few blastomeres formed lines, the majority did not. Therefore, we had to make this a more robust procedure. We took our clues for improving this method from the architecture of the embryo. Specifically, the cells are always grouped together and never found in isolation. Thus, to mimic this arrangement, we surrounded each blastomere with cells and proteins to simulate the geometry and molecular environment of the early embryo. The result was a much more efficient derivation procedure. Accordingly, we had developed the tools to perform the desired experiments. For the derivation procedure, we used 5 embryos that were donated by a single couple. From single blastomeres removed at the 3-day stage we derived a total of 9 human embryonic stem cell lines. Thus, the lines were either genetically identical or related. First, we had to prove that they had the characteristics that researchers think are common to all human embryonic stem cells. Specifically, they expressed a panel of markers that are associated with this state. Also, in a laboratory dish, they made descendants of the 3 lineages that give rise to all the cell types in the body. The most rigorous test of the stem cell state is transplantation into a mouse where these cells are able to differentiate further. Like those derived by conventional means, our collection formed many structures such as bone, muscle and glands. Therefore, we concluded that they had the same general properties as human embryonic stem cells that were derived from later stage embryos. Thus, we turned to the interesting question of whether they had unique properties. We characterized the cells at the genetic, epigenetic and functional level. At the level of genes, we compared the lines that were derived from a single cell of a 3-day old embryo to human embryonic stem cell lines that were derived by conventional means. There were striking differences in the gene expression patterns between the two. Epigenetic marks decorate DNA designating functional from nonfunctional areas in terms of gene expression. The lines that were derived from single cells had many fewer of these modifications suggesting less specialization. Finally, at a functional level, we showed that a subset of the lines we produced could spontaneously form cell types that are found in both the offspring and the placenta. Since these lineages diverge early in development, we took this as evidence of a greater degree of plasticity than is associated with the conventional lines. We think this property could be very useful for devising regenerative medicine therapies.
- Semin Reprod Med (2013) Human trophoblast progenitors: where do they reside? (PubMed: 23329637)
- Differentiation (2011) GROalpha regulates human embryonic stem cell self-renewal or adoption of a neuronal fate. (PubMed: 21396766)
- Biol Reprod (2011) Trophoblast stem cells. (PubMed: 21106963)
- Stem Cells Dev (2009) Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. (PubMed: 19222349)


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NetPicks Stock & Option Investment Advisory
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Note: We suggest that you read this Disclaimer since with any investment strategy there is always a risk of loss. You should be aware of these risks when trading in both stocks and options.


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Don't-Miss Macs Stories
Parallels 5 is a feature-rich and speedy virtualization solution that does an excellent job with DirectX and OpenGL video acceleration.
VMware's Fusion 3.0.1 adds many new features, including a full-screen toolbar, improved DirectX support, 64-bit native core, and a modestly revised interface to what was already a very good virtualization solution.
Boxee is now available to all and sports a new interface. Here are the details.
Some Gems become such an integral part of your Mac's day-to-day operation that you take them for granted. Dan Frakes revisits his Thanksgiving-day column to cover more Gems he's thankful for.
The Soulmen's Ulysses 2.0.4 writing app is dense, complex, and well-designed.
Password Assistant exposes OS X's built-in password generator
If you'd like to use OS X's built-in password generator to create secure passwords for Websites, online accounts, and disk images, Password Assistant provides easy access.
If you lose your iTunes library and need to copy files off your iPod and back onto your Mac, iTunes can't help you. But these 10 utilities can.
SpaceControl keeps an eye on your available disk space
SpaceControl watches your hard drives and notifies you when they're getting full.
If you're looking for software that's focused on recording and processing audio, Record should be on the top of your list to consider.
MiniUsage lets you monitor your Mac's activity
MiniUsage provides a useful systemwide menu for monitoring CPU usage, network throughput, and battery life.
LaunchCodes brings back 10.5-style document opening
LaunchCodes forces Snow Leopard to respect creator codes on documents you create so they open in the application that created them.
GoodNight sleeps your Mac after downloads
GoodNight can put your Mac to sleep when your download, or other network activity, is done.
Better integration with online e-mail servers and obvious iPhone sync features would have made SOHO Organizer 8 much more attractive as a personal information organizer.


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Do you have pets at home? If you do, then you probably feel a bit sad when you see your pets looking lonesome because they miss playing with you outdoors kapag tag-ulan. As pet lovers, one question that may cross your mind when the season of puddles is upon us is: how can you cheer up your pet when you're both unable to go out due to the rainy weather? Your precious cat won't even bother stepping out because it's wet outdoors. While your makulit dog or puppy is itching to take a walk and run around with you… what to do? Make your furry friend's home cozier and more fun for both of you to be indoors.
Because your friends from Team AyosDito.ph are pet lovers also, we want to share our pet-friendly shopping tips for fellow dog and cat-lovers out there.
We know that there are a lot of you because the pets for sale on AyosDito.ph keep increasing in number (and cuteness) so for this year's rainy season, Team AyosDito.ph is excited to let you into our pet lover's hearts with three AYOS tips on how to make your pet's home warmer, cosier and a whole lot more fun lalo na ngayong tag-ulan! With these tips, you'll see how the limited time spent enjoying the active outdoors doesn't have to be dry and dull for you and your beloved pets.
Make their bed cozy – nothing beats a cat nap on a soft bed. Di'ba kapag maulan, ang sarap mag relax sa bahay at matulog kasi malamig at presko ang panahon? How about gift your pets a cozy bed to rest in too!
- http://bit.ly/AYOSdogbed1
- http://bit.ly/AYOSdogbed2
- http://bit.ly/AYOSdogbed3
- http://bit.ly/AYOSdogbed4
Upgrade their toys – para hindi nila masyado mamiss ang free-running outdoors, compensate with fun and squeaky chew toys para may ibang magpaglibangan ang pets kapag makulimlim at maulan sa labas. Dahil indoors kayo, pwede ka rin mag potty train ng pets! http://bit.ly/AYOSkitty
Give them a fun bath – kung naabutan ng pets mo ang wet outdoors, chances are they would end up coming home with their muddy paws and coat. If the rainy season gives you the luxury to have me-time, extend your me-time with your beloved pets para sila rin squeaky clean and feeling cool while waiting for the next ray of sunshine to come in.
No pets yet? Check out our cute pets at AyosDito.ph! Not only are they bundles of joy, they are also a great source of comfort when the weather wears you down. http://bit.ly/AYOScutepets
Happy shopping!