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Immunohistochemistry - tissues
Immunohistochemistry - cells
Immunofluorescence - cells
Western blot
Protein array
Tissue microarrays provide the possibility to immunohistochemically stain a large number and variety of normal and cancer tissues (movie about tissue microarray production and immunohistochemical staining). The generated tissue microarrays include samples from 46 different normal tissue types from 138 individuals and 20 different types of cancer from 216 patients, each sample represented by a 1 mm core, in total 570 images. For each antibody, the protein expression pattern in normal tissue is represented by triplicate samples and protein expression is annotated in 76 different normal cell types present in these tissues. For cancer tissues each tumor is represented in duplicate samples and protein expression is annotated in tumor cells. Normally, a smaller fraction of the 570 images are missing for each antibody due to technical issues. Specimens containing normal and cancer tissue have been collected and sampled from anonymized paraffin embedded material of surgical specimens, in accordance with approval from the local ethics committee.
Since specimens are derived from surgical material, normal is here defined as non-neoplastic and morphologically normal. It is not always possible to obtain fully normal tissues and thus several of the tissues denoted as normal will include alterations due to inflammation, degeneration and tissue remodeling. In rare tissues, hyperplasia or benign proliferations are included as exceptions. It should also be noted that within normal morphology there exists inter-individual differences and variations due to primary diseases, age, sex etc. Such differences may also effect protein expression and thereby immunohistochemical staining patterns.
Samples from cancer are also derived from surgical material. The inclusion of tumors has been based on availability and representativity. Due to subgroups and heterogeneity of tumors within each cancer type, included cases represent a typical mix of specimens from surgical pathology. However, an effort has been made to include high and low grade malignancies where such is applicable. In certain tumor groups, subtypes have been included, e.g. breast cancer includes both ductal and lobular cancer, lung cancer includes both squamous cell carcinoma and adenocarcinoma and liver cancer includes both hepatocellular and cholangiocellular carcinoma etc. Tumor heterogenity and inter-individual differences is also reflected in diverse expression of proteins resulting in variable immunohistochemical staining patterns.
In order to provide an overview of protein expression patterns, all images of immunohistochemically stained tissue were manually annotated by a board certified pathologist or by specially educated personnel followed by verification of a pathologist. The pathologists are experienced in interpretation of tissue morphology under the microscope and have used a specially designed software to view and annotate the histological images. Annotation of each different normal and cancer tissue was performed using a simplified scheme for classification of immunohistochemical outcome. Each tissue was examined for representativity and immunoreactivity. The different tissue specific cell types included in each normal tissue type were annotated. For each cancer, tumor cells were annotated. Basic annotation parameters included an evaluation of i) staining intensity (negative, weak, moderate or strong), ii) fraction of stained cells (rare, <25%, 25-75% or >75%) and iii) subcellular localization (nuclear and/or cytoplasmic/membranous). The manual annotation also provides a summarizing text comment for each antibody.
The terminology and ontology used is compliant with standards used in pathology and medical science. SNOMED classification has been used for assignment of topography and morphology. SNOMED classification also underlies the given original diagnosis from which normal as well as cancer samples were collected from.
A histological dictionary used in the annotation is available as a PDF-document, containing images which are immunohistochemically stained with antibodies included in the protein atlas. The dictionary displays subtypes of cells distinguishable from each other and also shows specific expression patterns in different intracellular structures. Annotation dictionary: screen usage (15MB), printing (95MB).
Annotated protein expression aims to create a comprehensive map over protein expression patterns in normal human tissues and cells. The conflation of data from two or more antibodies directed towards the same protein target (non-overlapping epitopes), evaluation of the performance of respective antibodies and a review of available protein/gene characterization data, allows for a knowledge-based interpretation of the distribution pattern and relative abundance of proteins in various tissues. An annotation of protein expression is possible for all genes for which there are two or more antibodies directed towards the corresponding protein target.
The immunohistochemical staining pattern in normal tissues provides the fundament for a subsequent annotated protein expression. The annotation of an immunohistochemical staining pattern is subjective and based on the experienced evaluation of positive immunohistochemical signals in defined subpopulations of cells within a tissue context. The microscopical images and previous annotations of the included 82 normal cell types are reviewed simultaneously and compared. The annotation data is merged and results in a single expression profile for each protein. In addition to accounting for performance of antibodies and available protein/gene characterization data, the review also considers sub-optimal experimental procedures. This includes immunostaining errors such as sub-optimal titration of the primary antibody and suspected cross-reactivity as well as the fact that multiple immunostainings have been performed on non-consecutive tissue microarray sections, allowing for differences in immunohistochemical staining patterns caused by inter-individual and inter-specimen variations. The final annotated protein expression is considered as a best estimate and as such reflects the most probable histological distribution and relative expression level for the evaluated proteins, and is displayed as high, medium, low or not detected level of expression.
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As a complement to the representation of normal and cancer tissue, the protein atlas displays images of a selection of widely used and well characterized human cell lines as well as cell samples from healthy individuals and leukemia/lymphoma patients.
A cell microarray has been used to enable immunohistochemical staining of a panel of cell lines and cell samples. Duplicates from 46 cell lines,10 leukemia blood cell samples and 2 samples of PBMC renders a total of 116 cell images per antibody. Included cell lines are derived from DSMZ, ATCC or academic research groups (kindly provided by cell line founders). Information regarding sex and age of the donor, tissue origin and source is listed here. All cells are fixed in 4% paraformaldehyde and dispersed in agarose prior to paraffin embedding and immunohistochemical staining.
The CMA enables representation of leukemia and lymphoma cell lines, covering major hematopoietic neoplasms and even different stages of differentiation. Cell lines from solid tumors are also included in the CMA. A subset originate from solid tumors not represented in the TMAs, e.g. sarcoma, choriocarcinoma, small cell lung carcinoma, and the remaining cell lines are derived from tumor types also represented in the TMAs.
The immunohistochemical protocols used result in a brown-black staining, localized where an antibody has bound to its corresponding antigen. The section is furthermore histochemically counterstained with hematoxylin to enable visualization of microscopical features. Hematoxylin staining is unspecific, and results in a blue coloring of both cells and extracellular material.
In order to provide an overview of protein expression patterns, all images of immunohistochemically stained cell lines are annotated using an automated recognition software for image analysis. The image analysis software, TMAx (Beecher Instruments, Sun Prairie, WI, USA), built on an object-oriented image analysis engine from Definiens, utilizes rule-based operations and multiple iterative segmentation processes together with fuzzy logic to identify cells and immunohistochemical stain deposits.
Output parameters from the software always displayed in conjunction with the annotated images are:
As a complement to the immunohistochemically stained cells and tissues, the protein atlas displays high resolution, multicolor images of immunofluorescently stained cells. This provides spatial information on protein expression patterns on a fine cellular and subcellular level.
Originally three cell lines, U-2 OS, A-431 and U-251 MG, originated from different human tissues were chosen to be included in the immunofluorescent analysis. Starting from year 2012, the cell line panel has been expanded to include additional cell lines: A-549, BJ, CACO-2, HaCaT, HEK 293, HeLa, Hep-G2, MCF-7, PC-3, RH-30, RT-4, SH-SY5Y, SiHa, SK-MEL-30 and TIME. To enhance the probability for a large number of proteins to be expressed, the cell lines were selected from different lineages, e.g. tumor cell lines from mesenchymal, epithelial and glial tumors. The selection was furthermore based on morphological characteristics, widespread use and multitude of publications using these cell lines. Information regarding sex and age of the donor, cellular origin and source is listed here. For each antibody two suitable cell lines from the cell line panel are now selected for the immunofluorescent analysis, based on RNA sequencing data. The third cell line chosen for each antibody is always U-2 OS, in order to localize the whole human proteome on a subcellular level in one cell line.
In addition to the human cell lines, the mouse cell line NIH 3T3 is also stained. This is only done for the antibodies corresponding to genes where the mouse and human genes are orthologues.
Besides the HPA antibodies, the cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus, (ii) anti-tubulin antibody as internal control and marker of microtubules, and (iii) calreticulin for the endoplasmic reticulum (ER).
The resulting confocal images are single slice images representing one optical section of the cells. The microscope settings are optimized for each sample. The different organelle probes are displayed as different channels in the multicolor images; the HPA antibody staining is shown in green, nuclear stain in blue, micro-tubules in red and ER in yellow.
In order to provide an interpretation of the staining patterns, all images of immunofluorescently stained cell lines are manually annotated. For each cell line and antibody the intensity and subcellular location of the staining is described. The staining intensity is classified as negative, weak, moderate or strong based on the laser power and detector gain settings used for image acquisition in combination with the visual appearance of the image. The subcellular location is further combined with parameters describing the staining characteristics (i.e. smooth, granular, speckled, fibrous, dotty or clusters).
Knowledge-based annotation of subcellular location aims to provide an interpretation of the subcellular location of a protein in three human cell lines. The conflation of immunofluorescence data from two or more antibodies directed towards the same protein and a review of available protein/gene characterization data, allows for a knowledge-based interpretation of the subcellular location.
Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG/HSA-depleted human plasma and protein lysates from a limited number of human tissues and cell lines. Antibodies with a non-supportive routine WB have been revalidated using an over-expression lysate (VERIFY Tagged Antigen(TM), OriGene Technologies, Rockville, MD) as a positive control. Antibody binding was visualized by chemiluminescence detection in a CCD-camera system using a peroxidase (HRP) labeled secondary antibody.
Antibodies included in the Human Protein Atlas have been analyzed without further efforts to optimize the procedure and therefore it cannot be excluded that certain observed binding properties are due to technical rather than biological reasons and that further optimization could result in a different outcome.
All purified antibodies are analyzed on antigen microarrays. The specificity profile for each antibody is determined based on the interaction with 384 different antigens including its own target. The antigens present on the arrays are consecutively exchanged in order to correspond to the next set of 384 purified antibodies. Each microarray is divided into 14 replicated subarrays, enabling the analysis of 14 antibodies simultaneously. The antibodies are detected through a fluorescently labeled secondary antibody and a dual color system is used in order to verify the presence of the spotted proteins. A specificity profile plot is generated for each antibody, where the signal from the binding to its own antigen is compared to the unspecific binding to all the other antigens. The vast majority of antibodies are given a pass, but a fraction are failed either due to low signal or low specificity.
In total, 44 cell lines and 27 tissues have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene.
For cell lines, early-split samples were used as duplicates and total RNA was extracted using the RNeasy mini kit. Information regarding cellular origin and source of each cell line is listed here.
For normal tissue, specimens were collected with consent from patients and all samples were anonymized in accordance with approval from the local ethics committee (ref #2011/473) and Swedish rules and legislation. All tissues were collected from the Uppsala Biobank and RNA samples were extracted from frozen tissue sections.
For a total number of 86 cell line samples and 95 tissue samples, mRNA sequencing was performed on Illumina HiSeq2000 and 2500 machines (Illumina, San Diego, CA, USA) using the standard Illumina RNA-Seq protocol with a read length of 2x100 bases. Transcript abundance estimation was performed using Tophat v2.0.3 and Cufflinks v2.0.2. For each gene, FPKM values or 'number of Fragments Per Kilobase gene model and Million reads', were calculated and the average FPKM value for replicate samples were used as abundance scores. The threshold level to detect presence of a transcript for a particular gene was set to > 1 FPKM.
FPKM thresholds were further set for categorization of transcript expression levels into low, medium or high RNA abundance.
The RNA-seq data was used to classify all genes according to their tissue-specific expression into one of eight different categories, defined based on the total set of all FPKM values in 27 tissues:
For each gene, a protein evidence summary score was calculated based on three parameters: UniProt protein existence (UniProt evidence),
transcript profiling categories (RNA evidence) and a Protein Atlas antibody based score (HPA evidence).
The UniProt protein existence data was assigned to classes
The UniProt protein ids were mapped to genes from
Ensembl version 73.37.
The RNA evidence was based on the gene abundance level described in the RNA section above.
The HPA evidence was calculated based on the manual curation of Western blot, tissue profiling and subcellular location as described in
Supplementary Table 1 in Fagerberg et al.
The protein evidence summary score for each gene was assigned High if a gene was found having both UniProt evidence class 1 and High
in the HPA evidence; Medium if the gene had UniProt evidence class 1 or was scored High in the HPA evidence; Low if the HPA evidence
was Medium and the UniProt evidence class was 2, 3, 4 or 5; Only RNA if UniProt evidence class was 2 or RNA evidence was High; and None
if RNA evidence was Medium or lower and the gene was scored as UniProt evidence class 3, 4 or 5.
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Prep Softball: Sells leads North past Albemarle
SPENCER — The only sure way to keep North Rowan senior Taylor Sells from going 4-for-4 is to only let her get three at-bats.
Sells singled, homered and doubled Wednesday as the Cavaliers crushed Albemarle 12-1 in a 1A YVC game shortened to five innings by the 10-run rule.
Sells, who also was the winning pitcher, plans to sign with Catawba on April 10.
"Taylor could play several different positions at Catawba," said North coach Sarah VanBuskirk. "But her best position is at the plate. As a hitter, she can be lethal."
VanBuskirk was a .300 hitter for Catawba five years ago. She's in her first year as North's head coach after logging some experience as an assistant at Carson.
"I've been impressed with how good some of the teams in the YVC are," VanBuskirk said. "Maybe teams don't have as many good players (as 3A teams), but they're good where you need to be good."
North (4-5, 2-5) has beaten Salisbury twice, but it didn't get its first YVC win until it pounded South Davidson on Tuesday. Now the Cavaliers own two league victories.
"We're getting there," VanBuskirk said. "We're starting to improve game by game."
Sells struck out the side in the first inning, and North put up six runs in the bottom half of the first to take control.
Chasity Young, Lindsey Hinson and Brianna Mock walked to load the bases, and Sells nearly tore the left fielder's glove and leg off with a wicked single. The ball went to the fence to clear the bases, and Sells made it to third.
Kaya Teasley's groundout scored Sells for a 4-0 lead.
North eventully sent 11 batters to the plate in the inning, and Young's two-run single boosted the lead to 6-0.
Albemarle got its run when Alex Potter (3-for-3) led off the second with a home run. Sells answered with a solo homer of her own in the bottom half.
"I finally got a good pitch to hit that was actually in the strike zone," Sells said.
A hit by Teasley and several errors pushed North's lead to 9-1 before the second ended.
Albemarle loaded the bases in the third, but Sells got out of the jam with a bouncer back to the circle. The pitcher tossed to catcher Anna Lingle for a forceout.
Then Sells struck out two batters after the Bulldogs filled the bases in the fourth.
Sells, who fanned seven, felt discomfort in her right shoulder after the top of the fourth.
"I think it was mostly the cold," she said.
Sells was in some pain, but she also was due to hit, so she put on a helmet and gapped a double. That triggered a three-run inning. Teasley doubled, and Ivory Hunter, Lexis Gonzalez and Young had singles.
"Some games we've just been swinging and swinging," Van Buskirk said. "Pitch selection has been a journey, but we made some progress today."
Hinson pitched the fifth against the Bulldogs and got out of yet another bases-loaded jam to end the game.
"We had the bases loaded twice with one out and didn't score," Albemarle coach Doug Talbert said. "That's what you do when you're winless."

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Thu, 10/17/2013 - 09:55
Director of the Center for Marketing Technology Ian Cross has been selected as moderator at the Chief Digital Officer executive round table discussion on October 22. He will lead a discussion about new media, online marketing, and marketing technology hosted in Boston.
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Director of the Center for Marketing Technology, Ian Cross, explains the imperative need for marketers to embrace the concept of 'social business' when discussing direct marketing in the digital age.
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How To Make Sprinkles for Cupcakes, Cookies & Cakes!
A few days ago Stella, the blogger at BraveTart, blew my mind. She said that you could make sprinkles easily in your own kitchen. Crazy, I thought — brilliant! I obviously had to try it. Here's how it turned out for me, documented step by step.
Homemade Decorative Sprinkles
Now, you may be asking already: Why in the heck would anyone want to make their own sprinkles? Here are three reasons I can think of, off the top of my head:
1. For health - If you want to have complete control over everything that goes in your (or your kid's) mouth, then this is a great way to make something fun and pretty while certifying it is gluten-free, or free of specific allergens. I used standard red food coloring, but you could also use all-natural or vegetable-derived dyes.
2. For aesthetics - Need to match your cupcakes to your outfit exactly? This is your chance.
3. Because you can - And seriously, isn't this the best reason? To make cupcakes or cookies from scratch, completely, and be able to say, "Yeah, I made the sprinkles too" — them's bragging rights right there.
Stella explained that her reason for making these is that she is working for a bakery that wants to have everything made in house — even decorations. So that pushed her to come up with these DIY sprinkles. You can see her recipe here:
• Rainbow Sprinkles at BraveTart
This is basically a recipe for royal icing, piped into long thin stripes, and left to dry overnight. Then they are chopped into tiny bits, or sprinkles. Honestly, this is a piece of cake — so to speak.
Now, on to the action! I adjusted Stella's recipe a bit, cutting it in half, and using powdered egg whites instead of egg white.
What You Need
4 ounces (about 2 cups) confectioner's sugar
1 teaspoon Just Egg Whites
1 tablespoon warm water, plus extra as needed
1/4 teaspoon vanilla, almond, or lemon flavoring
Pinch salt
Parchment or wax paper
Food coloring paste (optional)
Pastry bag
Pastry tip with very small round hole, such as Wilton No. 2
1. Lay a piece of wax paper out on a clean surface. Gather your ingredients. Decide how you want to flavor your sprinkles. You can leave them unflavored, or use vanilla or another flavoring. I used Fiori di Sicilia from King Arthur Flour, which gave my sprinkles a distinct taste of Creamsicles.
2. Sift the confectioner's sugar into a medium-sized bowl. It's very important to sift the sugar in this case, because even the tiniest lump will clog your pastry tip later. So sift the sugar through a sieve or a sifter.
3. About Just Egg Whites, or powdered egg whites: This is a substitute for raw egg. You could also use about half of a raw egg white, but I find this stuff useful for royal icing and other recipes that call for raw egg white.
4. Whisk 1 teaspoon of the powdered egg whites together with 1 tablespoon of warm water. Whisk until smooth and foamy. Whisk in the flavoring and a pinch of salt.
5. Mix the egg white liquid into the powdered sugar and stir to combine. The mixture will probably look clumpy and thick, as pictured.
6. Add additional water in 1/2 teaspoon increments until the mixture is smooth and liquid, yet still thick.
7. I am not giving a specific amount of water here, because it depends on the powdered sugar and even the humidity of the day. But you want a smooth, thick, pipe-able frosting like the one pictured above.
8. Choose your food coloring, if using. It's best to use the paste or gel type of food coloring, as opposed to liquid. The colors are more vibrant, and they don't significantly change the texture of your icing the way extra liquid will.
9. Using the tip of a toothpick place a dot of food coloring in your icing mix.
10. Stir thoroughly until the color is completely distributed.
11. Set up your pastry bag with your #2 (or similar) tip.
12. Fill the bag and press any air pockets out of the icing.
13. Pipe out a long, thin line of icing on the parchment or wax paper. It's best to keep the line as straight as you can, but it doesn't need to be perfectly straight. (See how wavy mine is!)
14. Repeat until all the icing is used up. Important: Let the icing strips dry for a full 24 hours, or at least overnight. In winter, like now, I let mine dry for about 20 hours, and they were fine.
15. When they are completely dry, line them up evenly against the edge of a flat knife, like a cleaver or santoku.
16. Chop into small pieces, or longer ones for jimmies. Store in an airtight container until using.
I do have to say that these tasted pretty fantastic. No waxy, chemical aftertaste; they just tasted a bit sweet, with some mellow citrus flavor. Just think — what about coffee sprinkles? Or bacon? The possibilities are endless.
Has anyone tried these? Got any tips? Or ideas for natural coloring?
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(Images: Faith Durand)
Per serving, based on 2 servings. (% daily value)
44 Comments
I bet these taste better than those waxy things. Although I had some peppermint ones that I bought for Christmas (really pricey ones) that were awesome
this is totally fantastic. i'm allergic to food coloring and boy are those naturally colored sprinkles pricey! thanks for sharing!!!
those looks suspiciously like a homemade version of those delicious/terrifying fluffy grocery store sugar cookies. you know the ones. I need a post on this pronto.
OMG my mum had the same measuring spoons set as you only hers is bright orange! I wonder if she still has it now. <3
homemade sprinkles, flavored and colored how I want them? Yes, thank you!
O.O My little one can never eat sprinkles because he can't have artificial dyes (especially red!). I'm on this like Donkey Kong!
these looks awesome, but what about home-made chocolate sprinkles?
@likethunder my guess is that chocolate jimmies are made with a tiny bit of cocoa and lots of edible wax. If I were to try them at home I would either try a ganache with some wax mixed in (probably still going to be too soft, though) or use the method above and flavor it with this:
- Chocolate Extract
Faith, I LOVE that you offer me the chance to perfectly match my sprinkles to my outfit -- definitely a must for the next partay!
This sounds incredibly intriguing and fun. I've always hated the taste of sprinkles, but I'm sure these would be great. Maybe someday I'll give it a shot, not that I'm ever in the mood for sprinkles...
I've always hated the unnatural sprinkles too. But to answer the chocolate question, don't you think it'd be easier to just do straight up chocolate shavings, or pipe actual chocolate just like the icing and chop that up? In that vein, I'm thinking I'll try making my own sprinklies with naturally tinted white chocolate, because I like that flavor even better. (And I have this as a backup if that doesn't work.)
$5 to anyone who comes up with natural homemade silver dragées next. :P
I think a better tip for shape would be to pipe them onto a bed on 10x sugar. I think this would leave them a little heartier.
Great timing - I had been thinking about how I might want to do this sort of thing recently.
I'm sure these taste much better than the industrialized dye saturated ones sold in most stores.
Oh my gosh....my most favorite reason...Bragging rights!! hahahahaha, that's hilarious! love it!
I love the idea of this but I just can't see myself taking the time to make these. If I'm going to take the time to make something from scratch, I want it to have more impact than this. But I would be VERY impressed if anyone served me something with homemade sprinkles!
I live in a place where sprinkles are a rare find. Can't wait to try these out.
This is SO exciting! Must try those!
How long can you keep them though? Is it ok to make a batch in advance and keep it in the cupboard (fridge?) for a while?
I love these DIY posts...I can't wait to try this out this weekend!
You could always pour boiling water over red cabbage to get BLUE water to add. And add a little LEMON juice to make it RED.
So lovely.
Yeah, to piggy back on Ashvina's comment -- you could use dyed beet or cabbage water instead of plain water as you're adding liquid.
But, call me lazy, I'm not sure that I have the time to make homemade sprinkles AFTER baking a cake, making frosting, and decorating. Too many steps!
I saw this over the weekend and tried it – they were fantastic! My family thought I was crazy, though =)
I'm with ginnielizz:
"$5 to anyone who comes up with natural homemade silver dragées next."
They are still available online, with a note that they won't ship to CA, and that they should be removed before eating!
I saw this too and have wanted to try them, even though I have absolutely no need for them. And your suggestion of COFFEE flavor, that really makes me want to try it!
Bacon sprinkles?? Why not just... I don't know, bacon? Chopped into bits?
ts, silver dragees are illegal in California because some guy made it his life's mission. No joke. The only place I ever found them was at a small baking supply store, and the owner had to go in the back to get them and was very hush hush and mysterious about the whole thing.
I think I might try these. I've never cared for sprinkles, except how happy they look. And creamsicle flavor sounds delicious.
Please post the recipe for these cookies/frosting, they look perfect!
It would be a lot of work but,you can make homemade dragees. Make tiny sugar pearls with isomalt. Cover them with silver luster dust. Just a thought.The pink sprinkles are great.
foodefafa, I thought the exact same thing about the fluffy and sinful grocery store cookies. That said, PLEASE POST THE RECIPE. We're dying!
Thank you! Sometimes pulling out the measuring cups and confectioner's sugar is so much easier for me than putting the kids coats on and walking down to the store for the 4 yr old's precious sprinkles (that taste like wax to me.)
Sorry about the lack of response on the cookie recipe! I also am looking for the perfect soft, fluffy, cakey grocery store style cookie recipe, and this one just wasn't it. It looked good but it's not there yet.
If I do finally find the right recipe, be sure that I'll post right away!
I want to try out this recipe but i need to make alot of them in a short amount of time.
Would it work if I refrigerate them to get them to dry faster? If so, how long must they stay in the refrigerator to be completely dry?
Thanks so much, I really hope you can help me!
Can these be made sugar-free, and if so how would i do that?
this. is. AMAZING! will definitely try natural coloring w/ spinach, beets, carrots, ect. :)
How about cranberry juice for natural color and flavor instead of the water? Might be too light though...
@Yellow Coffee Cup -- In making naturally-colored icing I've found raspberry juice works the best. The easiest way to get some is to allow some frozen raspberries to thaw, then smush out the juice in a sieve.
This is one of the most brilliant posts I've ever read! I cannot wait to make these - so easy & no need to rush to the store to buy another manufactured product. Love it!!!
I'm thinking of soaking shredded coconut in cranberry, raspberry, or pomegranate juice. Quicker, less sugar.
Brilliant! Provided I use the organic powdered sugar, I can make these corn-free for my corn-allergenic friend! (most sprinkles have dextrose, which is a corn derivative.)
Hmm.. now to figure out a way to do these without the egg.
I've gotten good food coloring out of beet water. I boiled beets to eat, and then reduced the water that I boiled them in as much as possible. I used it in red velvet cake and it gave it a nice earthy light red-pink dye. (Not deep red like red velvet made with food coloring, but I would rather not have the chemical dyes.) You can't taste the beets.
Great idea. I'll just plan ahead and make a few basic colored sprinkles. I am thinking white chocolate can also be prepared this way. I don't have to run out every time I need it for a project.
Hi! I love the idea of bacon sprinkles. What are you thoughts on how to best make them?? How do I get the bacon flavoring in the icing?? Should I use bacon grease? Melted bacon fat?? Something else entirely?


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2014 Easton MAKO Fastpitch Softball Bat: FP14MK
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52 Product Reviews
Questions and Answers
Got a question about the 2014 Easton MAKO Fastpitch Softball Bat: FP14MK? Ask our team of experts, and they will respond within 24 hours.Ask A Question
When you buy a bat and it says -10, what does that mean? Is that good or bad? Whats the best number you can get?
The 2014 Easton MAKO Fastpitch Softball Bat (FP14MK) is a -10 bat. The -10 refers to length to weight ratio on the bat. For example: if a bat is 32" long and is a -10, it will weigh 22 oz. The -10 is simply the difference between the length and weight. This number has no relation to performance of the bat.
Do you make a 2014 MAKO in the 30" 20 oz. size?
Easton did produce a 30" 20 oz. model of the 2014 Easton MAKO Fastpitch Softball Bat (FP14MK), but we no longer have it in stock. We do have the 2015 model available in that size: http://goo.gl/doJYI7.
What does it mean when it says -10? Is that good or bad?
The drop size, -10, is the difference between the length and the weight of the bat. An example would be a bat that is 30" and 20 oz. is a -10.
Product Description
New for 2014! The wait is finally over for the next big bat to hit the market! The new two-piece, composite Easton MAKO provides elite fastpitch players with the edge they've been waiting for! The MAKO concept is simple: swing the fastest bat with the biggest barrel. This allows hitters to produce power unlike ever before! One of the secrets behind the MAKO's success is Easton's new TCT Thermo Composite Technology. It's engineered to provide top-notch players two major benefits: unmatched bat speed and a massive sweet spot. There's no doubt the MAKO's made to enhance performance! But that's not all! The MAKO will feel incredible in your hands with its patented two-piece ConneXion technology and performance diamond grip. Thanks to this technology, no energy is wasted and each hit feels as smooth as butter! Easton: Engineered for Glory! The Easton MAKO is approved for play in ASA, USSSA, NSA, ISA, and ISF. It is backed by Easton's Exclusive (12) Month Manufacturer's Warranty. Free Shipping!
About Easton
Fantastic Customer Service !!!!!!! Easy ordering, Great Price, Fast Shipping....Enough Said....
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A full-time life demands education that fits YOUR schedule. OHIO knows learning doesn't occur only in campus buildings—it can happen wherever students are! eLearning options help to make that possibility a reality. eCampus links you to an Ohio University education that works for you—it extends Ohio University quality education beyond the bricks.
Thinking about an online degree? Want to take an online course? Need a more flexible distance education solution? Visit the Future Students section to see what we offer and how to get started.
Are you already a student at Ohio University and need to connect with resources for distance learners? Go to the Current Students section to find what you need.
Other sections provide information and resources for online teaching to faculty and staff at Ohio University, information for alumni and friends of distance education, and resources of interest to businesses and the community.
See why Ann Paulins has "the most fun job in the world!" Read full story . . .


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You ain't seen nothin' yet!    |    about me. |    archive
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You ain't seen nothin' yet!
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All images posted on this blog have been found and collected from the internet and are presented as visual inspiration for those viewing. These images are not presented as my own work, unless I note it under the specific post.
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An ordinary jacket won't make the cut on game day -- support your team by wearing this G-III® men's Touchdown polyfill full-zip jacket. NFL® team graphics are positioned on the left chest and back, and the colorblocked design features your squad's signature colors. A ribbed waistband and cuffs team up to keep the cold out.
- 100% Polyester
- Banded cuffs and waistband
- Detachable hood
- Front pockets
- Full-zip jacket with fleece lining
- Made in China
- NFL team graphics on left chest and center back
- Officially licensed
- Team-colored body with contrast sleeves and piping
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Special event items are produced by manufacturers only after the outcome of a game or event. These are advanced sale items and will ship immediately after they are received in our warehouse.
Manufacturer direct items are shipped directly from the manufacturer. These items are not available for international or expedited shipping.
Customized items can be personalized with options such as your name, your favorite number, and/or designs. Some options may be limited by league rules.


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Criminal Minds: Season 5 DVD
Sale Price: $22.98
To Order by Phone Call 1-800-933-6249
- Additional Details
- Format: DVD
- Rating: Not Rated
- Number of Discs: 6
- Run Time: 977 Minutes
- Region: 1
- Aspect Ratio: Widescreen
- Studio: Paramount
- DVD Release Date: September 7, 2010
- Audio:
ENGLISH: Dolby Digital 5.1
ENGLISH: Dolby Digital Stereo [CC]
- Cast: Joe Mantegna, Paget Brewster, Kirsten Vangsness, Matthew Gray Gubler, Thomas Gibson, A.J. Cook, Shemar Moore
- Genre: TV Series
- Color: Color
- Includes:
Original Featurettes:
"Case Files"
"Fear The Reaper"
"Gubler Direct"
"Material Witness"
Gag Reel
On Air Promos
- Release Date: 2009
CRIMINAL MINDS revolves around an elite team of FBI profilers who analyze the country's most twisted criminal minds, anticipating their next moves before they strike again.
The Behavioral Analysis Unit's most prominent agent is David Rossi (Joe Mantegna), a founding member of the BAU, who returns to help the team solve new cases, while pursuing some unfinished business of his own.
Each member brings his or her own area of expertise to the table as they pinpoint predators' motivations and identify their emotional triggers in the attempt to stop them.
The Fifth Season features incredible guest appearances by stars of the big and small screens: Gavin Rossdale, Tim Curry, Forest Whitaker, C. Thomas Howell, DB Sweeney, James Fallon, Bud Cort, and Gabrielle Carteris.
6-disc set includes all 23 powerful episodes, deleted scenes, a gag reel, and exclusive featurettes you cant see anywhere else including a piece on the 100th episode and an interview with rocker Gavin Rossdale!
Bonus Features:
- "Case Files: Subjects Unknown, Break, Grim, Darkness, Dante and Deserted"
- "Celebrating 100"
- "Fear the Reaper"
- "Gubler Direct"
- "Material Witness"
- Two On Air Promos


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Wed, 07/11/2012 - 12:52
Professor of Management Susan Adams and Chair of the Computer Information Systems department Leslie Waguespac weigh in on the argument over the value of tenure.
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Director of Entrepreneurial Studies Fred Tuffile talks to BostInno about Bentley's strong Entrepreneurship Program. Bentley Alumni Scott Smigler is also highlighted for sharing his experience at Bentley as he built a business from his dorm room with the help of the General Business curriculum.
Bentley University
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From internet golf pioneer to industry leader, The Golf Warehouse (TGW.com) took its first order in April 1998 and became one of the world?s first online golf retailers. Starting as a small family-operated company with only four full-time employees TGW has since grown to approximately 250 team members. Now, with over 230,000 square feet of warehouse space devoted to a huge selection of golf related products, The Golf Warehouse has become Golf?s #1 Online Superstore.
In 2005, BaseballSavings.com and SoftballSavings.com joined the TGW family. With over 20,000 baseball and softball products are available to choose from, Baseball Savings? internet sales continue to increase and the first Baseball Savings mail-order catalogs mailed in 2010.
On October 28, 2010, SoccerSavings.com joined its golf, baseball and softball counterparts and with an ever growing inventory, great service and pricing is already experiencing incredible growth.
We hope you enjoy browsing through the roughly 40,000+ items available via our online golf catalog. The Golf Warehouse brings together a team of dedicated people who love golf and believe it is their job to provide enthusiastic customer service and support to golfers who demand the best performing, name brand products, at reasonable prices. A commitment that will assure that each and every customer has a rewarding and enjoyable shopping experience. With The Golf Warehouse offering so many name brands and quality products at the best prices, there is no need for golf enthusiasts to go anywhere else!
"I continue to receive excellent service and competitive prices from TGW!"
"TGW continues to be an easy, informative and cost effective way to compare and buy any golf related items!"
"TGW is easy to navigate. Great product descriptions and customer reviews are helpful."
"I've been purchasing from them for 10 years or more & have been satisfied every time."
"Great selections at TGW (much more than other golf product stores/sites)"
"Very easy to use. You have a wide variety of equipment which makes it a good one-stop-shopping experience!"
"I shop here all the time. It offers a huge variety of golf apparel for all budgets for women and that is hard to find! I really appreciate having tgw as a source!"
"I am completely satisfied with all aspects of this process. TGW's website is great and easy to navigate. The price was by far the best of all sites I checked out. My hybrid shipped on time and got here when they said it would. Nothing but praise."
To see a summary of customer ratings, gathered by an independent agency, visit Bizrate®, a leading resource for shoppers and retailers.
Security of your online transaction is paramount
at The Golf Warehouse. All transactions are taken over
a secure server connection. You may note the https in
the URL of our order submission page. This means that
your information is not accessible to everyone else. Any credit card information you enter on our site is strongly
encrypted by industry-standard protocol, SSL (Secure Sockets Layer). The Golf Warehouse Web site uses SSL to
communicate with secure browsers like Netscape and Internet Explorer. These browsers encrypt your credit card and
personal information in a way that makes it extremely difficult for anyone to intercept and misuse while being transmitted
between your computer and our server. Customers on a global scale can shop privately, confidently and securely.
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Click Here To Read Our Privacy Policy.
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By Mail:
The Golf Warehouse
Wichita, KS 67226
Enter Contest (By Mail):
To enter the Sweepstakes drawing, please fill out:
a 3x5 card on which you have printed your first and last name, street address and phone number and send to:
The Golf Warehouse, The Guide Outdoors Network Sweepstakes P.O.Box 3566 St Cloud, Mn 56397-3566.
Please enter once a month to increase your chances.
To obtain a copy of the Official Rules:
Send a self-addressed, stamped envelope to:
The Golf Warehouse, The Guide Outdoors Network Sweepstakes Rules
Dept R
P.O. Box 3699
St Cloud, MN 56397-3699.
Having The Right Equipment Is Essential!
Feel Confident In Your Purchase With Our Exclusive TGW Mulligan Club Playability Guarantee!
It's simple. Your complete satisfaction is very important
to us. In an effort to provide the highest level of
comfort when making a purchase, we are proud to offer
our exclusive 30 day playability guarantee. If for any reason your are not happy with the performance of
your club(s), simply click here and follow the instructions to begin the online return process.
This offer applies to items that are not damaged and
reveal only normal wear. The clubs cannot be altered in any way,
including shaft or grips. Limit one exchange per purchase.
Return shipping is the customer's responsibility. Shipping
costs are nonrefundable. Not available on custom club
orders. Replacement orders using in-store credit must
be placed over the phone. To reach customer service
call toll free 1-888-838-5551 in the US, and (toll call)
001-316-858-0470 for international customers.
Please Note: TGW Mulligan Club Playability Guarantee applies only to clubs with this label at the top of the page. It does not
apply to custom clubs or demo & returned clubs.
Purchase With Peace Of Mind!
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If a problem occurs with the product (clubs) and has been used in any way,
under normal wear & usage, the item will be handled as a warranty
issue, not as defective or under Playability Guarantee.
Return shipping charges for warranty items are the customer's responsibility.
All products carry a full manufacturer?s warranty. Should you have a problem
within the manufacturer?s warranty period The Golf Warehouse will do everything
possible to assist you in taking care of your particular situation.
If needed, call The Golf Warehouse to inquire on manufacturer's warranty time period.
It is at the manufacturer's discretion on repair, replace or refund
of item. Please allow 4 to 6 weeks for repair from the time the
manufacturer receives the item.
Manufacturer Warranties still apply to custom and
special ordered items. Special Order items and Custom Orders are not returnable.
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The Golf Warehouse stands behind every item we sell.
We are committed to having happy customers that not
only can?t wait to shop again but go out and tell their
friends about The Golf Warehouse. That?s why we offer a 100% MONEY
BACK GUARANTEE. In the unlikely event that you are unhappy
with your product simply return it within 30 days for
a 100% refund.
Items returned or returned for exchange must be accompanied by the original packaging
and materials. Items must be in NEW and UNUSED condition, except for Playability items. All returns will be inspected prior to credit
being issued. Credit will be issued for the purchase
price of the item(s) returned. Shipping costs are nonrefundable.
When returning or exchanging a product, all parts, pieces, accessories, manuals, warranty information, and printed materials must be in their original packaging with the product in order to be eligible for a full refund. Closeouts, custom orders, demo items, personalized items, open videos, DVD's and computer software are not returnable.
We recommend that you insure the item when shipping. Items must be in NEW and UNUSED condition, except for Playability and Wearability items.
CUSTOM ORDERS, DEMO ITEMS and PERSONALIZED ITEMS are
not returnable. SPECIAL ORDER items that are not regular
in stock items are also not returnable.
Please Note: All sales are subject to availability
of product from the respective manufacturer and/or vendor.
The customer is responsible for any additional duty,
taxes, brokerage fees and insurance fees that may be
assessed for returned items shipped internationally.
Shipping charges are the responsibility of the customer
for warranty replacements. The customer is also responsible
for any duty, tax or brokerage fees and insurance for
replacements. Closeouts, custom orders or demo items
are not returnable either domestically or internationally.
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The Golf Warehouse periodically updates the suggested retail prices
posted on its website. As manufacturers provide the latest
suggested retail price information to The Golf Warehouse, The Golf Warehouse
updates its website. At times, however, a manufacturer
may change a suggested retail price without notice to
The Golf Warehouse. Thus, the manufacturer of a particular product
should be contacted if you want to ensure that you obtain
the most up-to-date SRP information available.
If you have any questions or comments about The Golf Warehouse's
pricing or other policies, please contact us at email@example.com
The Golf Warehouse has great vendor partnerships that empower us to offer a vast selection of items at the lowest prices. Since we are not a wholesaler, vendors have requested that we do not fulfill an order if we have knowledge of intent to resell the products. Adhering to this policy will allow us to continue providing the best golf values & selection on the web. If you have any questions about this policy, please contact us at email@example.com.
The Golf Warehouse collects sales tax in the states of California and Kansas.
Although we, as the seller, may not obligated to collect your state's sales tax, this purchase is subject to state sales tax unless it is specifically exempt from taxation.
The purchase is not exempt merely because it was made over the Internet or by other remote means. Your state, if it imposes a sales tax, may require that you report and pay
the tax due on your purchase. Details of how to file a state sales or use tax return may be found on your state's department of revenue website.
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- Antique Jewelry
- The Ages
- Engagement Rings
- Antique Rings
- Diamond Jewelry
- So Much More
Antique Guilloché Enamel Locket Pendant
Talk about attention-grabbing and unique…high style color and design characterize this antique clam shell motif pendant with locket back. Sweeping curves of ultramarine blue guilloché enamel alternate with diamond studded rows of 18k yellow gold. In all, sixty-two (62) rose cut diamonds with an estimated total weight of .55 carats dot the dome shaped surface. The reverse reveals the locket back with glass cover and push-in gold rimmed frame. The locket compartment, lined with black fabric is best opened by a jeweler or if so desired, we can leave it open for you. A simple "O" ring as bale completes the period pendant.
Date: Circa 1860.
Chain sold separately. Just ask us about our selection!
Measurements: 1-1/4 inches (3.2 cm) in length by 1 inch (2.5 cm) in width. Weight of 8.9 grams (5.8 dwt).
Condition: Excellent.
Note: Diamonds were not removed to preserve the integrity of the setting. Weights are approximated by measurement and formula and may vary from actual weights. All metals are acid tested.
Forms of Payment
- VISA and MasterCard, Discover and American Express credit & debit cards.
- Personal checks drawn on U.S. banks only - Please allow 7 business days for clearance.
- Money orders, cashier's and traveler's checks - Please allow 7 business days for clearance.
- PayPal.
Other Forms of Payment
Bank Wire - For international orders and orders of $5,000 and over. Please contact us for more details.
Layaway information and for full details see Payment Methods.
Same Day Shipping - Order by 1 pm CST M-F!
FREE for All U.S. Orders - Standard 2 Day Shipping.
FREE for All International Orders - Standard 2-7 Day Shipping.
Full details at Shipping & Insurance.
Complete Money Back No-Risk Guarantee
Our Guarantee
If for any reason whatsoever you are not completely satisfied with your purchase, we will refund the FULL purchase price including U.S. shipping if charged. Remember, there is no charge for our regular shipping service!
International Orders - Due to customs and paperwork requirements, return shipping charges cannot be reimbursed.
Return Procedure
- Notify us of your intention to return the item within three (3) days of receipt. You may contact us by email or by telephone.
- Please ship back to us within another two (2) days after we receive notification from you.
- As soon as the item is received, a full refund is issued to you that same day.
More details at Full Guarantee including gift giving options.


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A slip-on you'll love. For everything from casual days in the office to easy-going outings around town, these Crocs will handle whatever situation you're in. Built on a Croslite sole with a canvas upper, the Santa Cruz men's loafer is lightweight and offers all the comfort you expect from Crocs.
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Justin Sayre's gay variety show The Meeting (headed to 54 Below in July) held its annual "Night of a Thousand Judys," where Carolee Carmello, Telly Leung and more Broadway stars paid their tribute to the legendary Judy Garland!
Bradley Cooper, Patricia Clarkson, Alessandro Nivola and more on bringing the popular play The Elephant Man back to Broadway.October 21, 2014
What makes Jez Butterworth's new drama The River so eerie? Find out from headliners Hugh Jackman, Laura Donnelly and Cush Jumbo, then see them in the new production beginning October 31 at Broadway's Circle in the Square Theatre.October 14, 2014
Broadway.com hits the red carpet at opening night of the new smash revival of On the Town with hard-hitting questions about tight sailor uniforms, the show's sexy premise and more.October 20, 2014
A new star is stepping into Hedwig's heels on October 16! Broadway.com chats with Dexter and Six Feet Under fave Michael C. Hall about getting fit for his Broadway turn in Hedwig and the Angry Inch.October 9, 2014
Nathan Lane, Stockard Channing, Matthew Broderick, Megan Mullally, F. Murray Abraham, Rupert Grint...and Micah Stock! Talking with the hilarious cast of Terrence McNally's It's Only a Play on opening night.October 10, 2014
Talking up Keke Palmer, Sherri Shepherd and Judy Kaye about joining the ball at the Broadway smash Cinderella.September 11, 2014
Get ready for an NYC adventure! Check out the stars of the upcoming Broadway revival of On the Town. Find out why this is a classic that sings, dances and can cook too!September 9, 2014
The Rockettes are kicking off the holiday season in style! See the leggy ladies rehearse a brand new musical number for the legendary Radio City Christmas Spectacular.October 10, 2013
Broadway.com catches up with Alex Sharp and the cast at the opening night of Simon Stephens' The Curious Incident of the Dog in the Night-Time.October 7, 2014
Do you love
me Dirty Dancing? Of course you do! Drop those watermelons and get a sneak peek at the new musical stage version of the classic film, which begins touring across the country on August 21. You won't wanna take your hungry eyes away!
Blythe Danner and the stars of The Country House share their thoughts about Donald Margulies' play about fancy people in the Berkshires.October 3, 2014
You Can't Take It With You is back on Broadway, starring James Earl Jones, Rose Byrne, Kristine Nielsen and Annaleigh Ashford. See what the stars had to say on opening night!September 29, 2014


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Romney's plan for the future is made up of backwards policies from the past
President Obama has offered Americans a clear and detailed roadmap to increase our energy independence, improve access to quality education, and grow a stronger economy built on "Made in America." This is the plan we need to move the country forward.
Mitt Romney, however, is banking on taking America backwards. As the President said to Romney in the last debate:
You seem to want to import the foreign policies of the 1980s, just like the social policies of the 1950s, and the economic policies of the 1920s.
Here's how Romney is projecting a 20th century plan onto the 21st century.
Foreign policy of the 1980s
Mitt Romney has repeatedly revealed that he's "stuck in a Cold War mind-set", using "blunt, swaggering language on the stump that at times has evoked the Cold War Era." As Slate's Fred Kaplan noted," Romney and his advisers "don't seem to understand ... the extent to which the world has changed" since the 1980s. Here's proof:
Romney called Russia our "number one geopolitical foe," and has repeatedly referred to Russians as "Soviets."
Romney wrote in his book that, "It was a lot simpler when it was just the Soviet Union versus the United States." Now, threats are "more varied."
Romney compared his desire to increase military spending to President Reagan's defense budget increases because Reagan "pressured the Soviets into a corner" with that level of spending.
Romney's foreign policy advisers have also referred to "Soviets" and even attacked the President for supposedly abandoning plans to put a missile defense site in "Czechoslovakia"—a nation that has not existed for decades.
Social policy of the 1950s
When it comes to women's health and women's rights, Romney is firmly committed to taking American women back to the backwards policies of the 1950s. Here's why:
Romney declared support for overturning Roe v. Wade—a move that'd "take away a fundamental right that American women have had for nearly 40 years." Calling the decision "one of the darkest moments in Supreme Court history," Romney said he hopes to "appoint justices" that would reflect "my preference that they reverse Roe v. Wade."
Romney endorsed a bill that would allow employers to "have free rein to pick and choose your medical care as he or she saw fit"--including coverage for birth control. Nearly 79 million American women received health care coverage thorough their employers.
Romney declared he'd "get rid of" funding for Planned Parenthood, a health organization that provides vital basic health care—including breast and cervical cancer screenings—to millions of women. And Romney even went so far as to call for defunding Title X—a program that provides family planning and preventive health services. As the Washington Post's Sarah Kiff notes, "In moving to eliminate Title X, Romney is venturing into a new territory" in terms of extremism. Even anti-choice groups haven't asked candidates to oppose Title X.
Economic policies of the 1920s
There is no question that Romney's economic plan would drive us back to the failed policies of the past that have helped create this country's major economic crises. Not only would he go back to the policies that contributed to the 2008 economic crisis—but his policies even mirror the top-down approach of the 1920s, which focused on tax cuts for the wealthy and rolling back the rules of the road for Wall Street.
Romney would roll back regulations that would help prevent another financial crisis. He has pledged to repeal the President's Wall Street reform, which helps protect consumers and ends the era of Big Bank bailouts. But, "he is nearly silent on how—without the regulation—he would prevent Wall Street from once again engaging in the risky practices that helped cause the 2008 financial crisis."
Standing on hay bales in Iowa, Romney declared that "corporations are people, my friend." And his policies, which could raise taxes on the middle class to pay for new tax cuts for millionaires, illustrates what his priorities would be if elected president. And Romney has proposed additional tax cuts for the wealthy. He has put forward $5 trillion in tax cuts weighted towards millionaires and billionaires, which could only be paid for by raising taxes on the middle-class.
To learn more about the clear choice in this election, check out each candidates plans here—then share the facts with your friends.


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Inspired by the lovely asymmetry of a cactus in bloom, this band ring is both organic and elegant. Like the natural world, it's more interesting the more closely you look at it. See Details
Gemvara jewelry is truly personal: you design it, choosing gems and metals to celebrate your story, and we craft it just for you. We'd love to help you find your perfect piece! (more)What quality are your gemstones?
They are all-natural and hand selected fine quality. (more)


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Light Grunge Texture Pack 1Texture ID: 997 | Gallery: Texture Packs
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Filesize: 29.21 mb
Save Money with Texture Packs!
Texture Packs are sets of 15 large textures (3300 x 2200) delivered in ZIP file format for only $10.
That's just 67 cents per image! We give you a huge discount when purchasing textures in a pack.
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Untreated severe mental illness and its negative consequences persist despite the existence of assisted outpatient treatment (AOT) laws in 45 states and progressive civil commitment standards in 26 states. In virtually every state, implementation of existing laws and standards is inconsistent at best and nonexistent at worst. Common obstacles to the use of existing laws and standards include:
Legal barriers• Incomplete or inconsistent understanding of how the laws and standards work
• Inconsistent application by judges
• Inadequate enforcement of court orders
Clinical barriers
• Hospitals, physicians and mental health professionals who are unaware of the laws and/or don't know how to use them
• Identification mechanisms that would enable hospital emergency rooms, law enforcement and others to immediately recognize individuals under court-ordered outpatient treatment
Official barriers• Perceived or projected fiscal impacts on local government
• Shortage of public personnel with knowledge or training in implementing the laws
• Opposition by the mental health officials charged with implementing the laws and standards
• Opposition from tax-funded protection and advocacy (P&A) groups
Personal barriers
• HIPAA/confidentiality obstacles
• Absence of committed or informed family members to act as advocates
• Perceived civil rights issues
States whose civil commitment standards do not currently include the need for treatment are shown below in gray.
To help the Treatment Advocacy Center in the fight to overcome barriers to treatment, sign up for our news and updates.


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The Famed Disgaea series goes next gen on PS3! With high-definition graphics, including beautiful high-res 2D sprite art and stunning spell effects, one of the most highly-acclaimed and beloved strategy RPG series is making the jump to the next generation of gaming with Disgaea 3 Absence of Justice for PLAYSTATION 3. Re-enter the world of Disgaea and experience all of the humor, zany characters, thrilling action, and unmatched excitement in this all-new over 100 hour adventure!
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Philippe Gilbert sprinted to fourth place for the BMC Racing Team Sunday after giving furious chase to a leading trio that escaped on a short climb near the finish of the opening road stage of the Tour de France.
Nervous Day
Gilbert took advantage of the chasing efforts of teammate Cadel Evans, who pulled the peloton along after stage winner Peter Sagan (Liquigas-Cannondale) followed the attack of race leader Fabian Cancellara (Radioshack-Nissan) and Edvald Boasson Hagen (Sky Procycling) caught the pair in the final kilometer. Gilbert said registering his second-best result of the season (after a third place at Flèche Wallonne and fourth on Stage 5 of the Tour of Belgium) capped a nervous day. "At 23 km to go, someone touched my wheel and I thought I was going to crash," he said. "I stayed up, but lost a lot of places. Then I dug deep to come back in the final two kilometers and took some risks in the last descent. It's a bit of a pity because I finally won the bunch sprint, but it wasn't for the win."
Van Garderen Still Best Young Rider
BMC Racing Team's Tejay van Garderen held onto the "best young rider" jersey by finishing 21st, one spot behind Evans, but in the same time as the stage winner. He remains fourth overall, 10 seconds behind Cancellara, while Gilbert climbed to seventh, 13 seconds off the lead. Evans is eight, at 17 seconds. Van Garderen said he, too, was fortunate not to be caught up in several small pile-ups that occurred in the final 25 kilometers – one of them caused by a spectator videotaping in the road. "The last 60 kilometers or so it got pretty hectic," van Garderen said. "Our mindset was to be at the front, stay together and try to stay out of trouble. Sometimes sticking your head in the wind a little bit is easier and safer than to try to behind behind the bunch."
Evans: Team Is Working Well
Evans himself stayed out of danger on the 198 km stage with help from Marcus Burghardt and later, 17-time Tour de France participant George Hincapie, who towed him up to fifth wheel as the climb began three kilometers from the finish. "Marcus took me along on the last flat section there to the bridge," Evans said. "We were doing 74 kilometers an hour just to move up on the flat." The defending Tour de France champion said the team had information that the wind might have been favorable to split the race near the end, which is why the BMC Racing Team took to the front as the pack closed in on a six-man breakaway that had been away nearly the entire stage. "As it was it was, it was a headwind, which makes it easy for everyone to stay on your wheel," he said. "But the team is working well and functioning well and I'm glad to get one road stage done and the routine going."


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Saint Patricks Day
Vinyl Green Beach Balls (1 dz)
Make your beach party even more fun with colorful beach balls like these Vinyl Green Beach Balls. This inflatable beach ball is perfect for pool parties, the beach, or outdoor events!
- 1 Dozen Vinyl Green Beach Balls.
- Balls Measure 11 Inches Inflated.
- Ball Measures 14 Inches Deflated.
- Vinyl.
- Green.


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Main Content
You're not the type to stay in one place. Gather some supplies, round up some friends and explore the coast with this Explorer backpack by Dakine. It features skateboard carry straps on the front, a padded laptop pocket and fleece-lined mp3 and cell phone pockets so you can feel connected to the "real" world. Or you can unplug everything and have an old-school adventure without the safety of technology. Either way, it'll be the time of your life.
- Adjustable skateboard straps
- Approximately 20" x 12" x 10"
- Dakine embroidery
- Dual side mesh pockets
- Fleece-lined sunglasses pocket
- Front zip organizer pocket with fleece-lined cell phone & iPod pockets and pen/pencil slots
- Imported
- Large main compartment
- Made of 600D polyester
- Padded adjustable backpack straps
- Padded back panel
- Padded laptop pocket
- Sternum strap
- Top zippered stash pocket
Special event items are produced by manufacturers only after the outcome of a game or event. These are advanced sale items and will ship immediately after they are received in our warehouse.
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text: I am researching, gathering media, quotes, and news buzz for an article I am writing at our website about Martin Archery and their recent announcements that a strong future for MA is being attempted. Is there a place allowable to post the link to that article here or is it improper use of the forums? I do not want an automated spam catch to grab my post and want an MTF staff member to clarify if this is ok? Thanks a lot guys. I have read the forum rules but there was no clarification other than signature links.


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The SHPRC is Stanford's Sexual Health Peer Resource Center, a one-stop shop for safer sex supplies, sex toys, sexual health counseling, and more. Our mission is to promote sexual health and healthy relationships across campus, both through our center and through community outreach. We're located on the 2nd floor of Vaden Health Center, and we're open from 12-6pm Mon-Thurs and 12-5pm Fri. If you can't make it to the center, give us a call at (650) SAFE-SEX or contact a counselor via Advice Chat during our regular hours.
All undergraduates and co-terms receive a $3 credit to the center every quarter, so it's worth your while to come by! You'll be surprised how far your $3 goes with our heavily subsidized prices – condoms are 10 for $1, and all of our goodies are amazingly affordable. Due to our funding structure, grad students are not eligble for a subsidy; however, we welcome grad students to enjoy our products and services. We carry a wide variety of condoms, lube, and toys for all genders, as well as pregnancy tests. Even if you're not in the market for condoms or sexual health advice, you're more than welcome to stop by the SHPRC just to check it out.
Location and Hours
The SHPRC is located on the 2nd floor of Vaden Student Health Center
Mon-Thurs 12-6pm, Fri, 12-5pm
Fall, Winter, and Spring Quarter, from Week 2-Week 10.
(We are closed during Finals Week.)
Products and Library
The center carries a huge variety of goodies, from condoms to massage oil to vibrators. Check out our Products and Library page to see a description and price list for the products we sell.
An Informal History
In the mid-1970s, a group of Stanford students started the "Contraceptive Information Center," located in the Fire Truck House. Soon, the group moved to Cowell, with a branch at the Fire Truck House, called "Ye Olde Safer Sex Shoppe." In the mid-1990s, in response to a need to educate students about and protect students from AIDS, broadened the mission from contraception to the full range of Sexual Health and voila – became the SHPRC. The class for the SHPRC counselors, as well as for the Bridge, the PHEs, and HIV counselors began around the same time, when the Psych department became more research-focused. Read more…
The SHPRC offers outreach programs for residences and student groups. Check out our Outreach page for more information!
Become a counselor!
We're always looking for new counselors to join our team! Find out how you can become an SHPRC counselor here.


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Mobile Phones > Phones > HTC > HTC Sensation XE
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Deals on Wilhelm Tell Express Routes
See the Beauty of Switzerland at 20% Off
Scenic trains in Switzerland allow you to see amazing sights right from your seat. Right now get 20% off your Swiss Scenic Train Reservations when you use coupon code SCENICRES. See more of scenic Switzerland, for less.Read the Terms and Conditions
From June 26 through 9:00am ET on December 31, 2014, enter coupon code SCENICRES to save 20% Off Swiss Scenic Reservations. Discount offer applies to the following Swiss Scenic Train Reservations: Wilhlem Tell Express, Glacier Express, GoldenPass, Bernina Express including train & bus reservation. Discount offer is not applicable on the Chocolate Train. One coupon code per booking may be applied to online orders. This offer is combinable with other coupon code / gift cards by calling our contact center. Offer is subject to change or extension without notice. No adjustments may be made on prior purchases. 20% off discount is only applied to the cost of the Swiss Scenic reservation and is not applied to the cost of the ticket or pass.


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Mt. Lafayette & Mt. Lincoln & Mt. Liberty & Mt. Flume
Greenleaf Trail --> Franconia Ridge Trail --> Osseo Trail --> Lincoln Woods Trail
Distance: 12.5 mi.
Elevation Gain: 3,298 ft. (overall) 4,552 ft (total)
Time: 2:12 (Hut) 3:18 (Lafayette) 3:58 (Lincoln) 5:56 (Liberty) 6:50 (Flume) 9:21 (Total)
At the start of the trail near the tramway parking lot, it was certainly fall. There were leaves on the ground and the temps were cool. The trail starts by going down along the highway in the woods while slowly gaining elevation. After a while it starts to turn left into a series of switchbacks as it works its way up the side of the mountains. At the top of the switchbacks, you pop out into Eagle Pass. The place is pretty interesting with a shear cliff on your left and views back behind you and up ahead. I will definitely hike through here again. You work through the pass and start turning to the right working up the backside of ridge. After a couple switchbacks and some turns, you start getting close to the Greenleaf Hut. As I neared the hut, I started to encounter snow along the trail in the trees. It was just warm enough that some of this was melting and falling down on the trail and of course me as well. When I reached the hut, I took a break to have a snack. I also took off my wet clothes and put on most if not all of my dry layers. I knew it was going to be cold and windy ahead, and didn't want to be trying to add clothes in that. The temp at the hut was showing at 32 degrees and the wind was pretty steady at 20-25 mph gusting to 40mph.
The trail passing between boulders
Eagle Pass
Encountering snow
Starting out from the hut, I went a bit slower than normal to keep from getting sweaty and wet in the cold conditions. Before long I was above the treeline and fully into the clouds, cold, and wind. Snow was starting to cover the ground more, but luckily not much in the trail. Rime ice was also forming too. The closer I got to the summit, the more ice and snow there was. I also started to see other hikers headed the other way.
Looks like we will be in the clouds
More snow on the ground
Rime on the summit
I didn't stay at the summit long since it was windy and there weren't any views. Far different from the only other time I was here. It was looking (and feeling) like winter here. Heading down to Lincoln went well and quick. Still plenty of snow, but no slick trails. That was nice since I was exposed on the ridge in the wind. When I got to the summit of Mt. Lincoln, I wasn't fully sure I was actually on it in the clouds. There is two humps at the peak, and I couldn't tell if I was on the higher one. Judging from past pictures, I was in the correct place.
Still cloudy on Lincoln
I made my way down to Little Haystack and stopped just past the Falling Waters trail where it heads into the woods. Time for another snack. Coming down from here was steeper than I expected, but when I looked back at the map, it seemed right. Through the section before Mt. Liberty, the temps were getting higher and snow was melting off the trees again and the ground was getting a bit muddy. It's almost like spring time now. I only saw one other hiker in this stretch (a possible thru hiker). The trail is easy through here and pretty interesting. It went by quick and the only harder bit is just before Mt. Liberty.
Between Lincoln and Liberty
Liberty below the clouds
On Mt. Liberty I was finally below the clouds, but the still filled the sky. I was pretty comfortable after removing a couple layers a bit back. I stayed here for a bit and had some more of my sandwhich, but knew I should keep going as well.
Flume ahead
Cannon to the north
Down from Liberty and up Flume are both a bit steep with some scrambles and lots of small rocks. Having been here before, I was expecting this, but I was getting a bit tired by the time I hit the 4th peak of the day. For the fourth time today, I had the summit to myself. Just as I was leaving, a couple was coming out of the trees having come up the FLume Slide Trail.
A little steep and a lot rocky
Mt. Flume summit
The Osseo Trail has a brief section that is a little steep, but quickly gets pretty gentle until the ladders. I took my time on the ladders with them being damp, but things went smoothly. After the switchbacks it was back into some hardwoods again with a little bit of dull yellow still on the leaves. It was like fall again. The trail is easy from here, but seems a bit long (it is). By the time you start thinking the brook on your right is a ways below you and you aren't dropping elevation enough, the trail soon heads dowm more and you reach the Lincoln Woods Trail. This straight boring bit at the end was made better by a couple other hikers I chatted with that were on their way out from a 3 day trip of the Bonds
Back to fall like conditions
Even with the expired foliage and cloudy day, this hike was still great. I will certainly do this again. In the end, I enjoyed the snow and wind and was surprised with how my legs didn't seem as tired as I expected.
For all the pictures from the hike, just go HERE


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ProTek R/C 5x10x4mm Metal Shielded "Speed" 1/8 Clutch Bearings (10)
With your purchase, receive at no cost:
This is a pack of ten 5x10x4mm metal shielded "Speed" 1/8 clutch bearings. ProTek R/C "Speed" bearings are high quality steel/chrome bearings designed for the R/C racing enthusiast.
These 5x10x4mm metal shielded bearings are the standard bearing for the 1/8th scale nitro clutches. Please check the manual that came with your vehicle for the correct size if you are not certain.
These are also used as wheel bearings in the Kyosho Lazer ZX-5 1/10th scale 4WD electric buggy.
ProTek R/C is an AMain.com brand.
These 5x10x4mm metal shielded bearings are the standard bearing for the 1/8th scale nitro clutches. Please check the manual that came with your vehicle for the correct size if you are not certain.
These are also used as wheel bearings in the Kyosho Lazer ZX-5 1/10th scale 4WD electric buggy.
This product was added to our catalog on March 27, 2006.
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Merdith Roach
I agree these are the best $1.00 clutch bearings I've tried by far. The metal shield stays in place unlike most rubber sealed bearings and stay smooth and free over time. I can take these out and clean them with some denatured alcohol and blow them out and re-oil them and keep on using them. I was tired of constantly loosing shields with other brands and throwing them away.
Brett Blum
Verified Owner
Great bearings! They hold together much better than a rubber sealed bearings. I have been running these in my clutch and they have held up for off-road so far. Another good protek product.
Anthony Misasi
Verified Owner
Excellent bearings. after a quart of fuel used my CB will free spend for over a min. Thats how smooth these bearings are.
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HOME Cleveland Browns Gear Cleveland Browns Kids Cleveland Browns Kids Kids Cleveland Browns Kids Kids Accessories (16 items) Product ID: 1344531
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You're the type of fan who can never have enough Cleveland Browns memorabilia. This plush mascot will look great in your fan cave or on your little fan's dresser. This little guy shows everyone that you're serious about your Browns fanaticism.
- 100% Polyester body
- 78% PE pellets/22% Polyester filling
- 8" Tall
- Imported
- Officially licensed NFL product
- Quality embroidery
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I was wondering if anyone might know why my 2 Shadowcats have lost poundage?
I have the 60 pound max limbs on them, one set are Barnsdale and the other the powertuff.
As they are now with new spec cables and strings bottomed out all I can get is 51-52 pounds.
At one time I had at least 3/8" before the limbs would bottom out at 55 pounds.
Is it they are just worn out? I really want to shoot back up at 60 pounds.
I shoot at least 500 arrows a week with these 2 bows.
Thank you for any help on this.


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Details and Shopping
We the Free brings us back to our down-to-earth, All-American roots, made mostly with casual cottons that have a lightly distressed and perfectly worn in feel.
*By We the Free, a line designed exclusively by Free People
*100% Cotton
*Machine Wash Cold
Measurements for Size Small:
Length: 23 5/8"
Bust (all around): 41 1/2"
Sleeve Length: 22 1/4"
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text: With technology that monitors ordinary road markings, Active Lane Keeping Assist helps detect unintended drifting off-course, and alerts you by vibrating the steering wheel. If you continue to drift, it can selectively brake individual wheels to help guide the car back into its lane. New enhancements help it to further detect and alert the driver to overtaking vehicles, vehicles you would need to overtake if you change lanes, and even oncoming traffic in the adjacent lane.


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RonBron the new owner of Stratics has posted a new message dedicated towards all those members of Staff that recently left Stratics Service. You can find the Message Here.
Ever consider becoming part of the Stratics Team? Now is your chance! Stratics is Hiring Editors. If you think you have the chops find out how to join Here.
Stratics will be fielding an Extra Life Event Team this Weekend, you can find out how you can support us and help a good cause by visiting our Stratics Team Page at Extra-Life.org. You can read more about this mission HERE.
FINAL FANTASY XI General Discussion
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here is a really great new song I recorded yesterday in the basement. writing it was a magical experience…I had the music part down and when I started writing lyrics they were initially about my depression and how it was pointless to try to get myself out of it because it would just happen again and I should probably just kill myself, etc. but then I got to the second part of the song and found that I wasn't really moved to write words like that anymore because I simply felt better. so the song is literally a conversation between my depressed self and my happy self.
I don't know, I think I'm probably bipolar. this shit happens all the time. hopefully one day I can afford antidepressants. until then…TRENCH PARTY lives!!!
hey everybody, I'm Trench Party here with another brand new song. it's about a gay dude coming out of the closet, inspired by that queen song "don't stop me now". I'm not sure if that's what that song is about but it sure sounds like it and it's totally awesome. queen rules. my shit is awesome too though and if you agree remember to tell your friends and whoever about my band Trench Party. support local kickass music yall
aight I'm outz
hello everybody! my name is Jake and this is the brand new EP of my solo-project trench party! it's called Vacation, it's 8 songs composed on keyboard, and it is drastically different from all my other stuff. regardless, it is my best effort to date and I'm incredibly excited to show it to you! please DOWNLOAD A .ZIP OF THE ENTIRE ALBUM or enjoy it for free at a vast variety of internet locations:
TRENCH PARTY presents…
»»»"sad gay dude"««<
from the album Decadence.
please enjoy the new trench party single entitled "sleep terror" (alternate title: "whatever it takes I know I can make it through"). it is perhaps my funkiest, most danceable song ever and is certainly not to be uhh missed! also freely savor my remix of the young jeezy/mannie fresh composition "and then what?"
LYRICS: trust yourself. you can't trust yourself when biology occurs and your fight or fight instinct is perturbed. when a bump in the night causes you to wake up screaming with your hands around the throat of the love of your life, you'll know I'm right. you can't even leave yourself unattended for a mere forty winks, for the primate in your brain has got itself a testy violent streak. you can't even not question reality; that ache of doubt is yours. what if you awake to find your life is over?
fuck you.
Buy the new TP album Decadence here via PayPal!!
why, you ask? I mean…because $10 is hella cheap, it's got twenty new songs, it's in an actual jewel case, shipping is free, it's great, and you need it!!!
you can get it for the special, limited time price of $6 if you make a $5 or more donation at this show:
here's the facebook thing for that. thank you!!!
it's another snow day, so here's a brand new song! Thanks, Keyboard!
the lyrics:
If I embrace my indifference for the outcome, I survive. arrogantly matching life's coldness is the only way to thrive. life can't tempt me with its fallible indulgences. I'm apathetic towards existence and your love will never change that. I have never felt anything for anything at all. I don't understand what people mean when they speak of pain and pleasure. everything's a trifle; you won't make me concede. I don't care for life or death and your love will never change that.
"decrepit, irrelevant" [free download]
here is a new song I made last night, composed and recorded entirely on my new keyboard (except the drum loop which I found on Phat Drum Loops (and I guess you could say the vocals technically weren't recorded with the keyboard, but you know what I meant and frankly I think you're just trying to make me look like a asshole.))
I am really happy with this one. I think you'll find it to be much different from mostly anything else I've done, certainly with Trench Party at least. I guess I should shout out Everclear for making this song possible. the lyrics are about being really old and realizing you can no longer really do anything to change the world anymore and you sort of have to be demoted to a spectator when it comes to that.