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Suppressive Effects of Plumbagin on Invasion and Migration of Breast Cancer Cells via the Inhibition of STAT3 Signaling and Down-regulation of Inflammatory Cytokine Expressions
Wei Yan1,2, Bing Tu1, Yun-yun Liu3, Ting-yu Wang4, Han Qiao1, Zan-jing Zhai1, Hao-wei Li1, Ting-ting Tang1*
1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2Wendeng Zhenggu Hospital of Shandong Province, Wendeng, Shandong 264400, China; 3Department of Gynecology and Obstetrics, Wendeng Center Hospital of Weihai City, Weihai, Shandong 264400, China; 4Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Bone Research (2013) 4: 362-370. doi: 10.4248/BR201304007
Objective: The aim of this study was to investigate the effects of plumbagin (PL), a naphthoquinone derived from the medicinal plant plumbago zeylanica, on the invasion and migration of human breast cancer cells. Methods: Human breast cancer MDA-MB-231SArfp cells were treated with different concentrations of plum- bagin for 24 h. The effects of plumbagin on the migration and invasion were observed by a transwell method. The expressions of IL-1α, IL-1β, IL-6, IL-8, TGF-β, TNFα, MMP-2 and MMP-9 mRNA in MDA-MB-231SArfp cells were detected using Real-Time PCR. MDA-MB-231SArfp cells were treated with plumbagin at different concentrations for 45 minutes. The activation of STAT3 was detected by western blot. Following this analysis, STAT3 in MDA-MB-231SArfp cells was knocked out using specific siRNA. mRNA levels of IL-1α, TGF-β, MMP-2 and MMP-9 were then detected. Consequently, MDA-MB-231SArfp cells were injected intracardially into BALB/c nude mice to construct a breast cancer bone metastatic model. The mice were injected intra- peritoneally with plumbagin. Non-invasive in vivo monitoring, X-ray imaging and histological staining were performed to investigate the effects of plumbagin on the invasion and migration of breast cancer cells in vivo. Results: The in vitro results showed that plumbagin could suppress the migration and invasion of breast cancer cells and down-regulate mRNA expressions of IL-1α, TGF-β, MMP-2 and MMP-9. Western blotting demonstrated that plumbagin inhibited the activation of STAT3 signaling in MDA-MB-231SArfp cells. The inactivation of STAT3 was found to have an inhibitory effect on the expressions of IL-1α, TGF-β, MMP-2 and MMP-9. In vivo studies showed that plumbagin inhibited the metastasis of breast cancer cells and decreased osteolytic bone metastases, as well as the secretion of MMP-2 and MMP-9 by tumor cells at metastatic lesions. Conclusions: Plumbagin can suppress the invasion and migration of breast cancer cells via the inhibition of STAT3 signaling and by downregulation of IL-1α, TGF-β, MMP-2 and MMP-9.
Keywords: plumbagin; breast cancer; invasion; migration
Full Text
Breast cancer is the most common cancer in women, and bone tissues are the most frequent sites for metas- tases. Up to 70% of patients with metastatic breast can- cer develop bone metastases that induce increased osteoclast activity, resulting in local bone destruction and skeletal complications, including pain, hypercalce- mia, and nerve compression (1-3). The development and outgrowth of these secondary lesions rely upon the intricate cellular and molecular interactions between
mammary tumor cells in the bone microenvironment. Tumor cells secrete signaling proteins, such as parathy- roid hormone-related peptide (PTHrP) (4), to promote the secretion of RANKL, the receptor activator of nuclear factor-κB ligand, in osteoblast cells that activates bone osteolysis induced by osteoclasts. The concomitant bone destruction releases various growth factors, including transforming growth factor-β (TGF-β) and insulin-like growth factor-1 (IGF-1), to further bind the receptors on the surface of tumor cells and enhance cell proliferation and PTHrP production through activation of the Smad/ MAPK signaling transduction pathways, thus establishing a vicious cycle of bone metastases (5). The most widely used treatments in skeletal complications of varied neo- plastic diseases are bisphosphonates (BPs) that block osteoclast activity; this application has been effective in decreasing formation of bone lesions, although this stra- tegy fails to induce the bone regeneration (6). Further- more, a growing number of case reports have demons- trated that long-term BP therapy might lead to osteo- necrosis of the jaw (ONJ) (7-10). Therefore, the search for treatment options that can effectively inhibit tumor growth and reduce bone destruction caused by tumor metastases with mitigated side effects is of pivotal significance.
Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), derived from the roots of the medical plant plumbago zeylanica, is one of the most investigated compounds. Recent studies have reported the antibacterial effects (11), anti-inflammatory effects (12), and anticancer activities of plumbagin both in vitro (13) and in vivo (14- 15). Previous studies have shown that plumbagin can inhibit tumor cell proliferation in vitro by inducing apop- tosis and autophagy of breast neoplasm cells (13) and the invasion of prostate cancer cells (14). Recent studies have also demonstrated that plumbagin can inhibits osteoclastogenesis and reduces human breast cancer- induced osteolytic bone metastasis (16-18), however, more in vivo evidence need to be supplemented and the relevant mechanisms still remain to be investigated. Therefore, the aim of this study is to investigate whether there are any suppressive effects of plumbagin on the invasion and migration of human breast cancer MDA- MB-231SArfp cells and to explore potential functional mechanisms. We thus established an animal model of breast cancer bone metastases via injection of breast cancer cells intracardially. A non-invasive small animal monitoring system in addition to X-ray imaging and histo- logical analysis were employed to monitor the curative effects of plumbagin on breast cancer bone metastases in real time.
Materials and Methods
Main reagents
Plumbagin and dimethyl sulfoxides (DMSO) were pur- chased from Sigma-Aldrich (St. Louis, MO, USA). For cell culture experiments, plumbagin was dissolved in DMSO at a concentration of 200 mmol·L-1 and stored in a dark bottle at -20 ℃. This stock solution was diluted further in cell culture medium immediately before use. For the animal experiments, plumbagin was dissolved in 5% PEG 400 at the required concentrations.
Cell line and cell culture
The human breast cancer cell line MDA-MB-231SArfp was labeled with red fluorescent protein (RFP), which was separated from breast cancer bone metastatic sites and exhibited propensities of bone metastases and was kindly provided by Prof. Jiake Xu (University of Western Australia, Australia) (19). The cell lines were generally inoculated in Dulbecco's Modified Eagle's medium (DMEM, HyClone, USA) supplemented with 10% fetal bovine serum (FBS) and 0.75 mg·mL-1 G-418 for selection of cells that showed stable RFP expression (20). Cells were passaged at 37 ℃ in a humidified atmosphere with 5% CO2.
Female BALB/c nude mice (n=20) were obtained from the Shanghai Slac Laboratory Animal Company [Certifi- cate No. SCXK (Shanghai) 2007-0005] at age 4-5 weeks and weighed approximately 20 g. All of the animals were maintained under SPF conditions at 22.0 ℃±1 ℃ room temperature with a 12 h light/dark cycle and 40- 60% humidity. All animals were unconstrained regarding drinking and eating.
The small animal non-invasive imaging system was im- plemented with an IVIS spectrum (Xenogen, Hopkinton, MA, USA). A digital X-ray camera was supplied by Faxitron (MX-50 desktop X-ray radiograph).
Transwell migration and invasion experiments
A transwell method was used as previously reported (21). Briefly, 24-well transwell plates (8 μm diameter, Corning, NY) were applied to measure migration. The basement membrane of the upper chamber was covered with Matrigel (BD Biosciences) at 0.3 μg·μL-1 per well in invasion experiment. Breast cancer cells (5×104 per well) were treated with varied doses of plumbagin (noted as PL below) and suspended in serum-free DMEM added to the upper chamber. DMEM containing 10% FBS was added into the lower chamber as a chemoattractant and incubated at 37 ℃ for 24 hours. Cells that had failed to migrate were removed from the upper chamber with swabs; the remaining cells on the bottom side of the basement membrane were fixed with 4% paraformal- dehyde for 10 minutes and stained with 1% crystal violet (dissolved in 2% ethanol) for 10 minutes. A random selection of 3-5 fields were photographed and counted under the microscope. All of the experiments were repeated at least 3 times.
Real-time PCR analysis
Twenty-four hours after treatment with different doses of PL on MDA-MB-231SArfp cells, RNA was extracted via a Trizol method to evaluate sample concentration and purity. A reverse transcription kit (iScript cDNA Synthesis Kit, Bio-Rad) was used to obtain cDNA from the extract- ed RNA by the reverse transcription method. The mRNA expression levels of relevant factors were assessed using Real-Time PCR (Reagent Kit: SYBR®Premix Ex Taq™, Tli RNase H Plus, Takara) according to the manufacturer's instructions. Primers required for the experiment are shown in Table 1.
Western blot
Whole cell extracts were prepared by lysing the cells in RIPA buffer (150 mmol·L-1 NaCl, 1% sodium deoxycholate, 0.1% SDS, 50 mmol·L-1 Tris-HCl pH 7.4, 1 mmol·L-1 EDTA,
1 mmol·L-1 PMSF, and 1% Triton X-100), containing a cocktail of protease inhibitors and phosphatase inhibi- tors. Protein concentrations were determined using the BCA protein assay (Thermo Scientific, IL, USA). A total of
Table 1 Primer sequences for Real-time PCR analysis
Forward primer(5'-3')
Reverse primer(5'-3')
Product Length/bp
20–30 µg of protein was separated by SDS-PAGE and transferred to a nitrocellulose membrane. The proteins were detected using primary antibodies targeting p- STAT3, STAT3, or β-actin (Cell Signaling Technology, MA, USA). Protein bands were visualized using an Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA).
siRNA transfection
MDA-MB-231SArfp cells were seeded in 6-well plates at a density of 1×104 cells per well for 24 h to reach sub- confluence. Next, cells were transfected with STAT3- specific siRNA or a scrambled siRNA control (Santa Cruz Biotechnology) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instruc- tions. The specific siRNAs consisted of pools of 3 target- specific 20- to 25-nt siRNAs designed to knock down the target gene expression.
Construction of the breast cancer bone metastatic model and fluorescence detection
The exponentially growing cell line MDA-MB-231SArfp was digested with 2% trypsin. Cell suspension was pro- duced by terminating digestion with DMEM culture medium, followed by re-suspending in PBS solution at
1 000 r·min-1 for 4 min. Trypan Blue staining showed cell viability to be greater than 98%. With PBS medium, cell concentration was adjusted to 5×106 per mL. A suspension of 0.1 mL per nude mice was injected into the left ventricle of 20 female BALB/c mice and random- ly assigned into two groups (n=10 each group) with intraperitoneal administration of 100 μL 5% PEG or
4 mg·kg-1 PL 5 times a week for 7 weeks. Non-invasive fluorescence detection was measured once per week and recorded.
X-ray imaging and HE staining
X-ray imaging was used in tumor-loaded nude mice 7 weeks after injection of tumor cells. Metastatic tumors were removed and fixed with 4% paraformaldehyde for 24 h, followed by a rinse of running water overnight. Tissue sections, which were decalcified in 10% EDTA at 4 ℃ for three weeks and embedded in paraffin, were made and observed by HE staining.
Immunohistochemistry staining
Tissue sections were dewaxed and rehydrated, followed by an antigen retrieval process. Primary antibodies of MMP-2 and MMP-9 were added at 4 ℃ overnight and inoculated with secondary antibodies after rinsing at room temperature for 1 hour. After DAB staining and microscopic observation, reactions were terminated followed by hematoxylin re-dyeing. Dehydration and xylene transparency were completed after differentia- tion by hydrochloride alcohol. The sections were sealed prior to microscopic observation. A selection of 3-5 random fields was photographed and counted by microscopy.
Statistical analysis
All data were processed via SPSS 13.0 software. Quantita- tive materials were presented as x±s. Statistical correla- tion was evaluated by SPEARMAN level with α=0.05 as the bilateral significance level. A P-value of less than 0.05 was considered to be statistically significant.
PL attenuates migration and invasion of breast cancer cells
Inhibition of PL by 2.5-10 μmol·L-1 on MDA-MB-231SArfp cells was observed via a transwell method and showed a significant dose-related suppression of in vitro migra- tion ability in MDA-MB-231SArfp cells (Figure 1A and 1C). Breast cancer cells harvested after pre-treatment of PL for 24 h were inoculated into a transwell chamber and covered with Matrigel gel for 24 h. These results indicate
Figure 1 Effects of plumbagin on the invasion and migration of breast cancer cells. (A and C) Plumbagin (2.5-10 μmol·L-1) suppresses in vitro migration of MDA-SA-231SArfp cells at different doses. (B and D) Plumbagin (2.5-10 μmol·L-1) decreases the invasion of MDA-SA-231SArfp cells and tumor cells show little penetration into matrigel at a concentration above 5 μmol·L-1.
that PL by 2.5-10 μmol·L-1 is capable of markedly inhibi- ting invasion ability of MDA-MB-231SArfp cells. Moreover, tumor cells could hardly penetrate matrigel gel to achieve the basement of chamber at a concentration more than 5 μmol·L-1 (Figure 1B and 1D).
Effect of PL on mRNA expression levels of IL-1α, IL-1β, IL-6, IL-8, TGF-β, TNFα, MMP-2 and MMP-9 of breast cancer cells
RNA was extracted and then processed using a Real- Time PCR reaction after treatment with PL at 5 μmol·L-1 and 10 μmol·L-1 on MDA-MB-231SArfp for 24 h. The results indicate that, in comparison with the vehicle group (PL-
0 μmol·L-1), mRNA expressions of cytokine IL-1α and TGF-β, which are secreted from breast cancer cells, decreased significantly. mRNA expressions of MMP-2 and MMP-9 from the matrix metalloproteinase family (when treated with PL-10 μmol·L-1) were also greatly downregulated; however, mRNA expression of IL-6 dropped but exhibited no statistical significance. The remaining statistically significant factors were not observed in this research (Figure 2).
Figure 2 Effects of plumbagin on mRNA expressions of cytokines secreted from breast cancer cells. After treatment of plumbagin at
5 μmol·L-1 and 10 μmol·L-1 for 24 h, mRNA expressions of IL-1α and TGF-β secreted from MDA-SA-231SArfp as well as MMP-2 and MMP-9. mRNA expression of IL-6, however, showed a decrease without statis- tical significance.
PL inhibits the activation of STAT3 in breast cancer cells
To explore the mechanism whereby PL inhibited mRNA expression levels, MDA-MB-231SArfp cells were exposed to different concentrations of PL for 45 minutes. Western blotting revealed that STAT3 activation was significantly inhibited after 30 minutes (Figure 3A). Furthermore, we observed that PL inhibited activation of STAT3 in a dose- dependent manner (Figure 3B). Subsequently, STAT3 in MDA-MB-231SArfp cells was knocked out by specific siRNA. Real-Time PCR results also demonstrated that the expression levels of IL-1α, TGF-β, MMP-2 and MMP-9 were decreased (Figure 3C).
PL attenuates metastases of breast cancer in nude mice
Breast cancer cells were administered intracardially into nude mice followed by a gain in fluorescence signaling in the third week, thus demonstrating the existence of metastases. Few nude mice in PL treatment groups displayed weak fluorescence signals during the fourth week. The appearance of metastases and the intensity of fluorescence signal of systematic metastatic sites in the vehicle group, which was greater in number and intensity of metastatic sites of the PL treatment group, were observed in systematic skeletons and organs between the fourth and seventh weeks (Figure 4A and 4B).
PL ameliorated osteolytic lesions caused by breast cancer bone metastases
X-ray imaging for euthanatized nude mice after 7 weeks demonstrated an increasing number of osteolytic lesions in the vehicle group, compared with a lower number and smaller area of bone lesions in the PL treatment group (Figure 5A). Knee joints of nude mice were selected for tissue sections, and HE staining showed obvious bone lesions in the vehicle group with a near complete disappearance of joint formation, as well as few remaining sections of articular cartilage in the tibia. In contrast, intact articular structures and a smaller degree of destruction of bone tissues were observed in the PL treatment group, with smaller lumps of tumor tissue merely visible in the canal (Figure 5B).
The suppressive effects of PL on secretions of MMP-2 and MMP-9 of breast cancer cells
Tumor tissue was removed from euthanatized animals for fixation, paraffin embedding, tissue section and immunohistochemistry staining for MMP-2 and MMP-9. The results showed a lower number of MMP-2 staining- positive breast cancer cells in the PL treatment group than the vehicle (as was shown in Figure 6A and 6B). Furthermore, tumor cells without treatment expressed high levels of MMP-9, which accounted for approximate- ly 50% of all neoplasm in the cells. By contrast, MMP-9 staining-positive breast cancer cells in the PL treatment group decreased markedly (Figure 6A and 6C).
Figure 3 PL inhibits the activation of STAT3 in breast cancer cells. (A) MDA-SA-231SArfp cells were exposed to 7.5 μmol·L-1 PL for 45 min and STAT3 activation was detected by western blotting. (B) Western blot analysis of p-STAT3 and STAT3 protein levels in extracts from MDA-SA-231SArfp treated with PL for the indicated times. (C) MDA-SA-231SArfp was treated with STAT3 siRNA or control siRNA for 48 h, and the expressions of IL-1α, TGF-β, MMP-2 and MMP-9 mRNAs were detected by Real-Time PCR. The results are expressed as the mean ± SD at *P<0.05.
Figure 4 Effects of plumbagin on metastases of mammary cancer in nude mice. (A) Few mice exhibited weak fluorescence signals in the plumbagin treatment group during the fourth week. An increasing number of metastatic sites in systematic skeletons and organs were observed from the fourth to seventh weeks per vehicle group. However, in PL treatment cases, the number and intensity of fluorescence signals of metastatic sites were both less than the vehicle group. (B) Quantification of luminescence activities in the two groups at *P<0.05.
Figure 5 Effects of plumbagin on osteolytic lesions of breast cancer bone metastases. (A) Systematic skeleton X-ray imaging. In the vehicle group, a number of osteolytic lesions were observed in nude mice; in the PL treatment group, fewer osteolytic lesions were exhibited with lesion areas smaller than the vehicle. (B) HE staining of knee joints of nude mice. In the vehicle group, destruction of bone tissue in knee joints was obvious with the disappearance of joint structures and few remaining articular cartilage areas in the tibia. However, intact articular structure and fewer destruction of bone tissues were observed in the PL treatment group with small lumps of tumor tissue merely visible in the canal.
Figure 6 Effects of plumbagin on secretions of MMP-2 and MMP-9 in breast cancer cells. (A) immunohistochemistry staining, where fewer numbers of MMP-2 staining-positive breast cancer cells are shown in the PL treatment group than the vehicle. Tumor cells without treatment expressed high levels of MMP-9, which accounts for approximately 50% of all tumor cells. By contrast, MMP-9 staining-positive breast cancer cells by PL treatment decreased markedly. (B-C) Quantification of MMP-2 and MMP-9 positive cells in the tumor tissues from the two groups.
Breast cancer bone metastases and resulting complica- tions bring tremendous difficulties to clinical treatment, thus significantly impacting patients' quality of life and survival rates (22). In this study, we found that plumbagin could suppress migration and invasion of mammary neoplasm cells in vitro and reduce mRNA expressions of factors associated with migration and invasion of tumor cells. Plumbagin can inhibit distant dispersal of breast cancer cells administered intracardially in vivo, especial- ly bone metastases and osteolytic lesions. Additionally, MMP-2 and MMP-9 secretion by tumor cells in metastatic sites is shown to be inhibited, and as a result the invasion ability of breast cancer cells is reduced.
In vitro, mRNA expression of IL-1α, TGF-β, MMP-2 and MMP-9 by MDA-MB-231SArfp cells can be suppressed by plumbagin. Previous studies have shown that the binding between IL-1 and IL-1R is considered to activate p38 MAPK and NF-κB signal transduction pathways, stimulat- ing expression of VEGF and VEGF-2 and thus facilitating the growth of tumor cells (23). Moreover, recent studies have demonstrated that Leptin is capable of inducing proliferation and migration of breast carcinoma cells, and IL-1 plays a pivotal role in the signaling network (23) comprised of Leptin, IL-1 and Notch. Therefore, mammary neoplasm disease progression can likely be attenuated through decreased expression of IL-1. In breast cancer bone metastatic cycles, TGF-β significantly enhances tumor development to release more PTHrP and thereby aggravates bone destruction (24). Recent studies have shown that curcuminoids can block TGF-β signaling pathways to inhibit mammary cancer bone metastases in nude mice (25), which is similar to the mechanisms of plumbagin investigated in our study. MDA-MB-231SArfp cells treated with plumbagin for 24 h, mRNA expression levels of MMP-2 and MMP-9 in the matrix metalloprotein- ases family was decreased. Positive expression of MMP-2 and MMP-9 in tumor cells plays a vital role in their migra- tion and invasion in vivo (26). MMP-9 is especially noted for decomposing the collagenous component of the ECM and devastating basic construction of collagen networks, thereby facilitating local invasion and distant migration (27).
STAT3 has been reportedly linked to tumor develop- ment and metastasis through induction of various genes responsible for cell proliferation, survival and carcino- genesis (28-30). In our experiment, we found that the activation of STAT3 was significantly inhibited when MDA-MB-231SArfp cells were exposed to different con- centrations of PL after 30 minutes. Furthermore, we observed that the expressions IL-1α, TGF-β, MMP-2 and MMP-9 were also decreased in MDA-MB-231SArfp cells while STAT3 was knocked out by specific siRNA. Thus, PL has potential anticancer activity against breast cancer, which is likely due in part to inhibition of the STAT3 signaling pathway. Although we did not investigate the mechanisms of plumbagin-induced apoptosis in this study, previous results have reported the suppressive effects of plumbagin on DNA binding viability of NF-κB and Bcl-2, as well as induced apoptotic abilities against mammary cancer cells (31). Plumbagin is able to trigger a G2-M arrest and autophagy by inhibiting the AKT pathway in breast cancer cells (13).
In conclusion, plumbagin is effective at attenuating the invasion and migration of breast cancer cells, decreas- ing distant tumor dispersal and reducing osteolytic lesions caused by bone metastases. As previous studies have also shown, future therapeutic applications of plumbagin are promising for the treatment of breast cancer bone metastases.
This work was supported by grants from the National Natural Science Foundation of China (No. 81172549), the Shanghai Science and Technology Development Fund (No. 11XD1403300), and the Opening Project of Shanghai Key Laboratory of Orthopaedic Implant (KFKT2011003), which are nonprofit foundations with no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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*Correspondence: Ting-ting Tang
E-mail: email@example.com
Received 09 September 2013; Accepted 17 October 2013
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Prophetic Words for My Nation and My City - Music Videos
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Embed rlsandford 1679 views 2010-10-02T00:25:20 This was an interview show for Denver Celebration, KRMT TV, Daystar Television Network. It opens with music by Pastors and Loren and Beth Sandford. Loren and Beth pastor New Song Fellowship, Denver, Colorado, www.newsongfellowship.org. Loren is the author of "Purifying the Prophetic", "Understanding Prophetic People" and "The Prophetic Church". Pastor Gary Hines is the interviewer.
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We enjoyed staying here. It's not very close to Philly, but the location worked for us. Not many choices in the suburbs. Clean, reliable, comfy firm beds. Very friendly with a late night check in. We would stay here again. No fee for parking ever. Didn't check the breakfast. Nice lobby area.
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Description: Chet at his best in '78.
Chet Atkins - "Autumn Leaves" - Download MP3 music or MP4 video:
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From the Solo flights album..listen to all the variations Chet manages to squeeze out of this beloved old classic.
"Mister Guitar" w/Boston Pops 1972.
Chet's arrangement of Autumn Leaves in the key of Bm . sorry for the high tempo at the beginning, I was maybe still a bit angry about the many mistakes I had...
I have been longing to do this tribute to autumn, but have been busy with work. Now there is a three day weekend, and I finally got it done! Enjoy!
Another one. I cannot get the feeling of nylon beauty for this one since my nylon string is a flamenco guitar and a real classical. So I decided to play it o...
Chet Atkins' arrangement of Autumn Leaves (Live 1978)
One of my top 10 favorite guitar pieces, ever. Finally got around to learning the whole thing (well, minus the vibraphone solo). I did this at 145 bpm, which...
as recorded by Chet ATKINS. capo at 3rd fret. E an A bass cords from an electric bass. Guitar: Gretsch 6122/1959 (wide neck). effect: mac OS/garage band. Enj...
This one goes out to my grad school (and IBM) mentor, who is moving onto the next stage of his career, back to California. Knowing he's not the world's bigge...
Autumn leaves - Les Feuilles mortes. Picking Version by Chet Atkins. Played by Jérémie Gent.
George Benson,Earl Klugh,Chet Atkins.
Autumn Leaves (Chet Atkins)
Autumn Leaves (Chet Atkins style KEY:Em ) played on a Caparison Horus HGS guitar with a front PU clean sound. This guitar is usually used with a distortion s...
Pascal PAULHE autumn leaves, les feuilles mortes, Ovation country artist, VIP preamp.
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Yesterday NBC News wrote about a new survey from staffing firm Mom Corps (great name), which found people would take a pay cut for more flexible work.
The article doesn't say how many people the group talked to, but "nearly half of working adults surveyed (45 percent) are willing to give up some percentage of their salary for more flexibility at work." More than half would consider starting their own business to achieve flexibility.
Flexibility includes flextime – the ability to set a variable schedule so long as you get an agreed amount of work done, usually with part of the day being non-negotiable – and working from home (one of my favorite things about writing for Money Talks News).
The study also found that those willing to trade salary for flexibility would accept a 9 percent pay cut, on average. That's more than 50 percent above last year's result (5.8 percent).
While a lot of people want flexible work, only a small number of jobs offer it. "Only 3 percent of wage and salary employees work mainly from home," NBC News says.


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It's tough not to feel at least a little sorry for Cory Schneider. After the New Jersey Devils acquired the netminder from the Vancouver Canucks, the days of Schneider playing second fiddle to a veteran incumbent seemed to be over. New Jersey still had Martin Brodeur on the roster, but no one expected the over-the-hill legend to provide much resistance to the incoming Schneider.
Yet Schneider will sit on the bench for the third consecutive game tonight as the Devils continue to roll with Brodeur. While he's been playing the part of a professional just as well in New Jersey as he did in Vancouver, the frustration seems to be mounting for the netminder.
@Ledger_NJDevils Cory Schneider says he's frustrated not to be playing. "It's like Ground Hog Day for me."
— Rich Chere (@Ledger_NJDevils) November 27, 2013
It's a strange situation for the Devils, because they are justified in starting Brodeur since he's played so well over the last couple of weeks. Schneider is also justified in his aggravation over not playing despite the fact that Brodeur has been handed two straight losses.
If this was the first time that this had happened to Schneider, odds are he'd be more comfortable with it. He's been patient for a long while though, having spent four seasons backing up Roberto Luongo in Vancouver before managing to kind of steal the starting gig away. Even when he seemed to be the go-to guy for the Canucks though, he was still embedded in a pretty even time share.
After failing to find a trade partner for Luongo, Vancouver shocked the hockey world by shipping Schneider to the Devils for a first-round pick.
This made sense for New Jersey, as Brodeur's best years were clearly behind him. He's had a resurgence this season though, and has forced Schneider onto the back burner. Again.
Still, New Jersey mad it clear that Schneider wasn't going to come in and make 65 starts while Brodeur scooped up the rest. These are interesting comments by Schneider, and the net situation in Jersey just became a lot more interesting.


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In that case it is worth trying Main Concept AVC if it's available in VMS, it will do 2-pass encoding at VBR. I haven't used Vegas for a long time and don't remember all the options.
Try raising the profile and set Entropy Encoding to CABAC, also try 2-pass encoding.
Dave Baker commented on Enhancing only part of my video
It's easy in your NLE, you just add whatever corrections/enhancements you wish to the clips that need it.


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Dubstep Christmas Lights
Posted by Break.com Staff on Dec. 02, 2012
Posted by Break.com Staff on Dec. 02, 2012
My fav xmas lights show ever!
He's been making dubstep >:) no toys for him!
Amazing work. People need to quit acting Jewish and knocking it just because they chose dubstep
People can't help but be opinionated and mean unfortunately.....I think it was an amazing light show plus Skrillex made it even better. This had to of taken a lot of time and effort. Very nice.
Dubstep? Umm... NO.... Skrillex. Get it right.
The dumbest way to make money online - Rich45.com
I would cut the wires.
Bah Humbug!!
Awww, here I thought it was going to be "Pumped Up Kicks" Dubstep Remix with Dancer Marquese Scott. So disappointing.
that electric bill must suck..
Yeah, that's about how my brain feels when I'm forced to listen to dubstep...
Now that is just freaking awesome.
lol twitching in bed trying to fall asleep!
Dubstep kills puppies.
..and that Star of Bethlehem looks like an asshole...a sphincter vibrating to the sound of dubstep..
whole neighborhood is out of power
Grow up.
I have puppies.. and I like dubstep... and I listen to dubstep in the house... with the puppies in the same room... and they are alive... further argument is invalid


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New name, look and prices for Toronto's Bixi
Bike-sharing plan to unveil changes on Monday, which taken effect on Tuesday.
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It's bye, bye Bixi.
Toronto's bike sharing network has a new name, a new logo and new prices favouring long-term users.
The Toronto Parking Authority unveiled the changes Monday morning and they'll take effect Tuesday, the same day TPA officially takes over as the new operator.
The program will simply be called "Bike Share Toronto" and will be operated daily by Alta Bicycle Share , a Portland, Oregon-based company operating bike share systems in New York, Chicago, Boston and Melbourne, Australia.
The new logo will look almost identical to Toronto Parking logos except with a bike instead of a green P, said Marie Casista, vice-president of real estate, development and marketing for TPA.
"(The name) really represents what it is," Casista said, "and what we're doing."
The beleaguered urban cycling program, which started in 2011, has struggled financially, telling the city last year it could no longer make payments on a $3.9-million city loan.
In December, Bixi's creator Quebec-based Public Bike System Company and the city came to a deal to transfer all Toronto assets , with TPA set to run the program as of April 1. In January, the Public Bike System Company filed for bankruptcy protection.
The new pricing scheme is designed to encourage riders to commit for the long-haul, Casista said.
Twenty-four-hour passes will increase from $5 to $7 and 72-hour passes will increase from $12 to $15, while monthly memberships will drop more than half from $41 to $18 and annual memberships will decrease from $97 to $90.
While the TPA partnered with Alta Bicycle Share for daily operation, Casista said they're still searching for a sponsor to help offset the costs, which could mean another new name.
"If that were to occur then the name will change, but this could take some time," she said and in the interim the TPA thought the basic name "made a lot of sense."
With files from Robyn Doolittle
Correction - March 31,2014: This article was edited from a previous version that misstated the new name of the bike sharing program.
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Joakim Soria open to being a setup man with the Yankees
Nov 5, 2012, 9:31 PM EDT
Joakim Soria recently became a free agent after the Royals declined his $8 million option for 2013. Coming back from Tommy John surgery, the thought is that he'll look for a closer job on a one-year deal in hopes of re-establishing his value and testing the market again next offseason. But he's keeping his options open.
Soria's agent, Oscar Suarez, told Andrew Marchand of ESPN New York today that his client would be willing to pitch as a set-up man for the Yankees if he could learn from his idol Mariano Rivera.
"If the Yankees call, we will be all ears," Soria's agent, Oscar Suarez, said by phone Monday. "If there is a fit, Joakim would be elated to work with Mo. He would close everywhere except there."
Suarez told Marchand that he has already heard from eight teams, all of them contenders, but Yankees general manager Brian Cashman has yet to inquire. It appears that Soria's interest in working with Rivera is genuine and it would make for a pretty natural changing of the guard beyond 2013, but as we've seen with other free agents over the years, it never hurts to have the Yankees involved in the bidding.
Soria, 28, has a 2.40 ERA, 1.04 WHIP and 160 saves over five seasons in the major leagues. His 181 ERA+ is fifth all-time among pitchers with at least 300 innings pitched. Rivera is first all-time with an ERA+ of 206.
- vallewho - Nov 5, 2012 at 9:40 PM
could be good, if Soria can still sling it.
- tigerman3 - Nov 5, 2012 at 9:44 PM
Forget the Yankees they have Rivera comming back , come to the Tigers , you will have a shot at the closers job.
- jolink653 - Nov 5, 2012 at 10:13 PM
Give him a call…Would love to see this guy in our pen
- Gardenhire's Cat - Nov 5, 2012 at 10:19 PM
This is a much smarter move than overpaying for a 37-year-old Rafael Soriano (sorry Boras, but it's true).
- slartibartfast4242 - Nov 5, 2012 at 10:38 PM
Coming off of Tommy John, who's to say he can beat out Robertson for the set-up role? He might not be all that open to be relegated to middle relief purgatory.
Of course the Yankees don't need to say any of this to him. If he agrees to a reasonable deal (stay out of it Hank & Randy), it will provide great depth/ insurance to go along with Robertson, Joba and Aardsma.
- pisano - Nov 6, 2012 at 12:51 AM
His agent is trying to get the Yankees to bite so they can drive the price up on this guy, as posted above, who's to say if he 100% coming off of TJ surgery.
- budjoy4 - Nov 6, 2012 at 7:18 PM
I believe that Soria can help the Yankees.. there is no guarantee Rivera will regain his form at age 43. I believe Soria could be a good investment particularly in the 7th or 8thy innings and if he returns to form as a follow up closer to Rivera.
- doctorfootball - Nov 6, 2012 at 3:31 AM
Am I wrong or did this guy reject trade offers to the Yanks in the past? Why does he want to go there now?
- drunkhistorian - Nov 6, 2012 at 8:11 AM
I don't think Soria has ever had the ability to block a trade. Royals were just too stupid to move him.
- geoknows - Nov 6, 2012 at 10:08 AM
RTFA before you comment. The article specfically says he had a no-trade, and that the Yankees were on it.
- churchoftheperpetuallyoutraged - Nov 6, 2012 at 8:59 AM
Trade blocks/no-trade clauses are often used to sweeten the deal for the "blocked" teams.
- bobwheel - Nov 6, 2012 at 10:57 AM
No thank you. Robertson will be just fine in that role.
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Forum - View topicEncyclopedia Suggestion List
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Posts: 3906
|When auditing genres and themes, I would really like the ability to add a comment and see others' comments, like how other error reports work. I know the comments are needed for other error reports because a staff member needs to make a decision based on them, and that they aren't necessarily needed for audits because the system processes them automatically. However, I think comments would be useful to explain to the other auditors what you were thinking when you marked something as correct or incorrect. Especially when you are the first person to mark a theme as incorrect, it seems like it would really be helpful to be able to give an explanation as to why you think it is incorrect. Sometimes themes are just obviously incorrect, but often I mark a theme as wrong for some other reason, including that it is a minor theme and not a reason for watching the anime, or it is a non-visual theme and the instructions say that they should be visual themes. Also, sometimes I believe a theme is wrong, but want to give an explanation as to why because I see how someone could think otherwise (e.g. I would certainly say Slayers isn't a "magical girl" anime, but would want to give an explanation as to what I think the term "magical girl" means, and why I think it wouldn't apply to someone like Lina Inverse).
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Baka Ranger
Posts: 2490
Thinking on the information coming out of Japan with recent events, with agencies reporting that all of their talent are safe, I was thinking that it would be helpful if the agencies' company pages could reflect the seiyuu who work for them, and links on the seiyuu pages to reflect who they work for.
The upkeep of the pages could get complicated when people change/leave agencies, but a precision of "former" could be added on the agency page.
It would help a lot with the lists of people confirmed safe, to be able to list "All seiyuu from [agency] reported safe" with a link to the agency's page and the list right there.
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Baka Ranger
Posts: 2490
Inspired by this thread, how should the encyclopedia address DVD extras?
It would make sense for the extras to be listed under trivia, but when you have cases like Chevalier d'Eon and Fruits Basket where the extras were removed, do those go under trivia as well, or should they be listed as a note "The [company/line] release of [title] does not include [noted extras]"?
I think it would be interesting to have the info on the extras somewhere, but the changes would be important to someone deciding if they're going for the newer or older releases (which, while TRSI does generally list them, other sites are less reliable about listing them, especially if you're buying from other consumers).
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Encyclopedia Supporter
Posts: 3304
Location: ::Points to hand::
|I think that the releases page for given a disc usually does a pretty good job of documenting the extras.
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Posts: 1239
|When adding opening and ending themes, it would be helpful to contributors if the artist/group were automatically added as staff for the task theme song performance. Like this, we wouldn't need to input similar data twice.
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Posts: 1
|I'm trying to familiarize myself more with the various Japanese voice actors in animes I watch by looking up their pages and looking to see what other roles they have had that I have seen. It would be nice if the same icons that show up in the lists of animes that correspond to whether you've put it in your viewed/viewing list would show up in the view of the actor's various roles. This would more easily identify the entries that I am interested in.
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Posts: 105
It would be interesting to have a setting on the "Staff Member" pages where the "Staff In" column could display the anime/manga titles in order from Highest-to-Lowest Rated, with Alphebetical Order as the main option.
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Posts: 842
Dear Sirs and Madams,
Even though I am far from being anywhere close to true specialists in anime which have thousands of titles watched, I still managed to see more than three hundreds anime works, and this experience made me thinking about few things that might improve usefulness of ANN encyclopaedia anime/manga pages that would make lives of anime lovers easier and more satisfying:
Some viewers might prefer to explore anime/manga that is more unusual comparing to the most of other works. With the current rating system there is no way to know how typical or atypical certain anime/manga is.
For example, Kaiba is one of the most atypical anime series ever created, while Bleach might be one of the most typical (a teenage schooler finds out that he has superpowers -- check, spirits -- check, samurai-type warriors -- check, a la Men-In-Black an organization that fights bad spirits -- check).
This is totally separate matter from viewers' overall satisfaction (or lack thereof) from watching these anime. Being less typical does not mean that such title will be necessary as entertaining as typical anime (thus "Bleach" rightfully might have higher overall rating that many more original titles). So current rating system is very useful, but yet with certain addition it could save people a lot of time of trying to find less typical anime/manga.
Viewers might be offered to vote on how much they find certain anime/manga typical or not -- with, for example, the following options (of course, the words could be different, but the essence is the same): "typical", "less typical", "atypical".
With that kind of voting any newcomer could choose "Top 10/50/100 atypical" (or however this may be called) list in the ANN's menu and find out titles like "Hickaru no Go" because such work is different comparing to myriads of other anime, which may have the same or even better overall rating (for example, "Vandread" has better rating than "Yakitate!! Japan", but there is less originality in the former so viewer might forget this anime soon, while the latter might be remembered more brightly). Projects like "Kaiba" would be in the very top of the list.
Of course, this voting, brought to the "Top ..." type of results, should be weighted to exclude "noise" and possibly votes from users which watched less than certain quantity of anime titles -- this is matter of fine-tuning.
No matter how much good overall viewer rating is, some viewers might not get very interested in projects that are based on "story of the week" principle -- even if the stories are really good (like, for example, in "Mushishi", "Kino's Journey", et cetera), let alone most of other series, which have mediocre small plots, fillers which are irrelevant to the big-scale story (if it exists at all).
From my experience, this problem even more severe than the first one, because it is much harder to guess if anime/manga has real story developed within its 13/26/52/... episodes or just frustrating pointless disconnected filler with only half of even quarter of episodes actually developing the story.
This problem less related to manga, but even there sometimes it is applicable: for example, most of "Cardcaptor Sakura" manga is based on "story of the week" principle. But the whole project is so charming that overall rating is high (I personally enjoyed it but there may be others which find slow development of "big story" to be tiresome).
On this one, I am less sure: this could be handled either by additional voting, too, with question (only to these who watched anime/read manga completely) like "How many episodes (among *all* of them) of this anime/chapters of this manga are based on "story of the week" principle?" with options for answer like: "none", "not many", "significant", "about half", "most of".
Or this could be managed via addition of certain fixed characteristic to manga/anime, which could be set by editors and/or by experienced users which help editing the encyclopaedia. This decision, however, might bring disputes in some cases, even though this characteristics usually should be quite obvious.
It is understandable that whatever additions to "mechanics" of the site might make UI too cluttered and the DB overloaded, but proposed features are possibly unique among presented on similar resources and capable of making ANN fans even more grateful and devoted.
What you think?
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Posts: 2
|It would be really helpful if there was an option, in the encyclopedia search, to leave out anime in our "seen all" list. This way, if we're looking for new anime to watch, we won't have to double-check if we have seen an anime if we don't remember all the titles
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Posts: 1239
|It would be interesting if old official websites, instead of just being removed from the encyclopedia, gained a link to the Internet Archive. Websites of specific titles could be relocated to the links section of the encyclopedia while websites of companies and people could gain parenthesis or brackets indicating they come from the Wayback Machine.
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Posts: 13
|I'd like to see whether other people have reviewed a title on the 'submitted anime' and 'submitted manga' lists - that way, we could add our opinions to others on titles we know, which would then lead to more being marked as having enough opinions on them to be reviewed.
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Posts: 2113
Location: Where the rain is.
Here on this great site that is ANN, we can search through anime organized alphabetically, by genre, theme, ratings, objectionable content, or even by what year it came out.
I would like to propose adding a feature that lets us search through titles by episode count.
Sometimes I specifically want a short 12-13 ep show, and other times I want something longer than the standard 24-26 episodes without it breaking the 100 level. What Im thinking is that it wouldn't be like searching through titles by ratings, where every title is listed from highest rating to lowest. It would probably be best if it was more like the objectionable content search, where you could check a box saying you don't want it to show any shows with more than 100 eps, or not shows with less than 13 eps. That way you could narrow the list of titles down to shows that are a length which appeals to you. It would be an excellent addition to the advanced title search.
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Baka Ranger
Posts: 2490
For people who are members of a group (JAM Project's members, bands who have members with solo projects, etc), can we get two things?
1) A list of members (and, preferably, former members) of the group
2) A link to the group from the individual's page
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Shiroi Hane
Encyclopedia Editor
Posts: 6038
Location: Wales
|I think basically, we need an anthology structure for people.
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Baka Ranger
Posts: 2490
Yeah, pretty much.
It would be nice if we could use the anthology structure for agencies, too *points to suggestion near the top of the page, too*.
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RIGHT TURN ONLY - By Request
Sometimes current debates spill over into manga releases in the strangest ways. Join Rebecca as she checks out new and upcoming volumes of Spell of Desire and Vinland Saga, along with the manga adaptation of this season's most hotly debated anime and several more! ― You never can tell what you'll find when you're unpacking. Sometimes it's horribly melodramatic stories written in middle school (I can...
Shelf Life - Four Your Eyes Only
This week, a special club for a special aesthetic, grave diggers, and good ol' hijinks.
- Dr. Slump Movies 1-5
- Meganebu
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- Dr. Slump Movies 1-5
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- Sunday without God ― Shelf Worthy Sunday without God Complete + OVA BD Rental Dr. Slump Movies 1-5 DVD Perishable Meganebu Complete Series BD This may sound like an insignificant thing, but a few years ago, I turned off the notification sound on my iPhone for new emails. Leavin...
ANNCast - Klein Times
Vice President of New Generation Pictures Jonathan Klein returns to the podcast to discuss his two latest projects, the conclusion of Hellsing Ultimate and Bayonetta: Bloody Fate! Plus: stories from the old days, the dub scene in LA and Twitter time! ― ANNCast 203 - Klein Times Get the Flash Player to see this player. Vice President of New Generation Pictures Jonathan Klein returns to the podcast to...
A Certain Scientific Railgun S DVD
A Certain Scientific Railgun S' first half is much more action-packed than its predecessor and focuses more on Misaka than on the girl group dynamic of the earlier season. ― How much you enjoy this second series (whose "S" subtitle is explained in episode three) may depend on whether you preferred the action-oriented moments of the first season or the humorous slice-of-life aspects. If you are a fan ...
Anime Spotlight - Hi☆sCool! SeHa Girl
In order for Dreamcast, Sega Saturn, and Mega Drive to graduate SeHaGaga Academy they must complete one more assignment. Do they have what it takes to get it done? ― Hi☆sCool! SeHa Girl Visit the Official Site › Story Characters Trailer Cast & Staff Gallery Broadcast Begins October 8, 2014 (JST) Key Staff Original Creator:SEGA (Hero Bank (TV), Valkyria Chronicles (TV), Virtua Fighter (TV) ) Director...
The Mike Toole Show - Let's Talk It OVA
Mike takes a tour of obscure OVA releases from the early 90s. Hammerpants not included. ― Twenty years ago, everything had started coming up Tenchi Muyo!. The series actually kicked off in Japan in 1992, but went global in '94, and just kept on truckin'. There was an OVA series, then another OVA series, then a TV series and more OVA stuff and a movie and another TV series and another movie and anothe...
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Manga Dogs' first volume is, overall, a very funny look at those whose greatest desire/delusion is to be a mangaka. ― If Manga Dogs seems like a bit of a departure for Ema Toyama, that's because it is. Her fourth series to be published in English – TokyoPop published Pixie Pop and Del Rey/Kodansha brought us I am Here and Missions of Love – is a much funnier, more cynical story than her cute romances...
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Terrifying tresses! Maniacal manes! Fiendish follicles! Rampaging ringlets! Just try not to wig out. ― Hair has a long (pun intended) history in cultures around the world, and Japan is no exception. Only women in the upper echelons for society were allowed to wear their hair long while poor classes were required to tie up their hair. Boys were given a specific hairstyle to acknowledge their entry in...


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Water Chesnuts Whole 20 oz
Whole Peeled Water Chestnut 20 oz. Water Chestnuts are a crisp, starchy, slightly sweet, neutral-flavored aquatic vegetable. Extremely popular as an ingredient in stir-fries, they can also be chopped and added to soups, salads and rice, used as appetizers, or eaten raw A good source of fiber and vitamin B, they also contain calcium, iron, potassium and zinc, and are a flavorful alternative for dieters looking to enhance their meals. From Dayat. Product of China.


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Judith Fifield, professor of family medicine and director of the Ethel Donaghue Center for Translating Research into Practice and Policy (TRIPP), has been examining ways that faith communities can promote weight loss among their members since the late 1990s.]]>
To facilitate researcher collaboration, CHIP and the UConn Health Department of Psychiatry are co-sponsoring a Mental Health and Health Behavior Networking Luncheon on November 12, from 11:30am to 1:30pm. This luncheon will be held in the Demo Room at UConn Health, located at 400 Farmington Avenue, Farmington CT.
Attendees are invited to give a two-minute presentation with a single slide on their research interests, expertise, and what they are looking for in a potential research partner. Lunch will be served.
If you are planning to attend this event, please register at the link: www.chip.uconn.edu/chip-psychiatry-event.
For more information on this seed grant opportunity, please visit: www.chip.uconn.edu/seedgrants.
If you have questions about this event or seed grant opportunity, please email Kelsey Barton-Henry at firstname.lastname@example.com.]]>
Research has found that some obese patients are reluctant to seek medical attention because of feeling judged or disrespected about their weight, says Rebecca Puhl, deputy director and senior research scientist at the Rudd Center and senior research scientist at CHIP, who is joining UConn's Department of Human Development and Family Studies.]]>
Researchers at the Connecticut Institute for Primary Care Innovation (CIPCI) think that we may be able to reduce hospital readmissions through improvements in doctor-to-doctor and doctor-to-patient communication. CIPCI has approached CHIP for support in the research and evaluation of new mechanisms for reducing hospital readmissions.
CHIP hopes to form a team of researchers to evaluate the efficacy of these interventions. Participants are required to attend bi-monthly meetings, aid in the construction of effective interventions. If you are interested in contributing to this project, please get in touch right away as the next meeting will be held at 6:00pm on Tuesday, October 28th. Please contact Kelsey Barton-Henry at firstname.lastname@example.com .]]>
Professor Kim Gans, who joined UConn's Department of Human Development and Family Studies this semester, is one of only two researchers nationwide bringing such interventions to home-based childcare, and the only researcher providing such interventions in both English and Spanish.]]>
This year, CHIP and CCMC have jointly created two seed grant opportunities to support research in Children's Health Behavior. Two grants of $30,000 each will be awarded to teams of dual-PIs, one from CHIP and one from CCMC.
The October 9th event is designed to inspire connections between researchers in the area of Children's Health Behavior for application to these seed grants. At the event, researchers will be given the opportunity to present (with 1 Powerpoint slide) on their specific interests, the types of projects on which they are interested in working, and the expertise they are seeking in a potential collaborator.
There will be time for informal networking and, of course, refreshments!
RSVP here: www.chip.uconn.edu/chip-childrens-event
Questions? Contact: Dr. Georgine Burke at 860.837.5268
If you plan to present using a Powerpoint slide, please send it to firstname.lastname@example.com by October 1, 2014.]]>
For Immediate Release: Sept. 12, 2014
EAST HARTFORD, Conn. – Connecticut's flagship public university today announced a new partnership with the Rudd Center for Food Policy and Obesity, a nationally recognized nonprofit research center dedicated to informing policy decisions surrounding obesity prevention.
Officials from the University of Connecticut and the Rudd Center discussed the center's new affiliation with UConn during a ceremony at Goodwin Elementary School in East Hartford that emphasized the importance of research in preventing obesity and improving the health of young people.
According to the Centers for Disease Control and Prevention, one in three children in the United States are obese or overweight, causing both immediate and long-term effects on their health and well-being.
"The Rudd Center has developed an outstanding national and international reputation for sound science and strategic policy advocacy," said Mun Choi, provost and executive vice president for academic affairs at the University of Connecticut. "We are thrilled to have the Rudd Center join UConn as we build a growing record of excellence at our institution."
Previously located at Yale University, the center and its faculty will move to UConn in January 2015. It is one of the first major initiatives of UConn's Academic Vision, which prioritizes health and wellness research as an integral part of the University's mission.
Recently ranked as one of the nation's most effective nonprofits working on nutrition policy, the Rudd Center is a leader in conducting cutting edge research to inform pressing public policy issues; its work is widely used by policy makers and health advocates.
The Rudd Center is currently anchored by four core faculty members, all national experts in their fields: Marlene Schwartz, Rudd Center director; Rebecca Puhl, deputy director; Jennifer Harris, director of food marketing initiatives; and Tatiana Andreyeva, director of economic initiatives.
"We are excited to join UConn and the community of world-class researchers whose work is relevant to childhood obesity and weight stigma," said Schwartz. "By joining UConn during this monumental time of growth, the Rudd Center will remain a leader in addressing how home environments, school landscapes, neighborhoods, and the media shape the eating attitudes and behaviors of children."
The move will allow those researchers to expand their work and build new collaborations with UConn experts on nutrition, public policy, psychology, agriculture, economics, and obesity – many located within the University's Center for Health, Intervention and Prevention (CHIP), where the Rudd Center will be situated. CHIP, which is led by Jeffrey Fisher, Board of Trustees Distinguished Professor of Psychology, has received more than $100 million in external funding to support its health-related research, and has a proven track record of fostering interdisciplinary collaborations between many of these research areas. Its Obesity Research Group boasts 130 members from more than 20 UConn departments and multiple campuses.
Importantly, the Rudd Center's relocation to Hartford's Constitution Plaza, near UConn's Graduate Business Learning Center, positions their resources near Connecticut policymakers. Rudd Center researchers have participated in meetings with the White House Task Force on Childhood Obesity, provided expert testimony in state and senate legislative hearings on proposed laws, and their research has provided essential support for federal, state, and local governments and NGOs in establishing evidence-based public health policy. Their research grants hail from government agencies and private foundations, including the Rudd Foundation, National Institutes of Health, U.S. Department of Agriculture, Robert Wood Johnson Foundation, American Heart Association, and Horizon Foundation.
The alignment between UConn and Rudd provides a new platform for researchers to elevate their work on obesity, investigating such varied questions as: economic incentives and the role of marketing in food choices; genetic and neurophysiological moderators of risk for obesity; chemosensory perception in humans and how it influences food preference and intake; worksite health promotion programs; weight management interventions for adults and children; faith-based interventions; identifying food deserts and measuring health outcomes in those areas; effects of cholesterol-lowering medications on muscle performance; obesity prevention policies; and weight-based stigma and bullying.
CONTACTS: Kristen Cole, University of Connecticut
(860) 486-2997 or firstname.lastname@example.com
Megan Orciari, Rudd Center
(203) 432-8520 or email@example.com
* * *
About the Rudd Center for Food Policy and Obesity
Press Kit materials — released 9/12/14


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Dr. I-yeh I. Gong, MD
Hematology/Oncology, UC Davis Comprehensive Cancer Center
- 4501 X St Fl 2
Sacramento, CA 95817
- 3 Office Locations
- Email a Friend
- (916) 734-5959
Dr. I-yeh I. Gong has been practicing medicine for 17 years. Dr. Gong graduated from the University of California, San Diego School of Medicine in 1997. He practices medicine in Sacramento, CA and 2 other locations and specializes in Hematology/Oncology and Medical Oncology. Dr. Gong is affiliated with VA Central California Healthcare System and UC Davis Medical Center. He speaks English and Spanish.
Gender: Male
Years in Practice: 17 Years
Languages Spoken: English, Spanish
Medical School: University of California, San Diego School of Medicine
Graduation Date: 1997
Hospital Affiliations: VA Central California Healthcare System, UC Davis Medical Center
Specialties: Hematology/Oncology, Medical Oncology
*This physician has multiple office locations. See Appointments for details.
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WebMD does not provide medical advice, diagnosis or treatment. See additional information.


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Los Angeles Lakers (14-21)@
Houston Rockets (22-13)
Wednesday, January 08
Attendance: None
Game Leaders
25 pts, 50 fg%, 100 ft%
38 pts, 61 fg%, 73 ft%
12 reb
13 reb
13 reb
8 ast
4 ast
4 ast
4 ast
Houston, TX (SportsNetwork.com) - James Harden poured in 38 points on 14-of-23 shooting and spearheaded Houston's second-half surge to lead the Rockets past the struggling Los Angeles Lakers, 113-99, at Toyota Center.
Dwight Howard and Terrence Jones each posted double-doubles, with Howard totaling 20 points and 13 rebounds and Jones adding 15 points and 13 boards in Houston's second straight victory.
The Lakers had three players score 20-plus points, but are still mired in a 1-9 slump. Nick Young netted 25 points off the bench, while Pau Gasol and Jodie Meeks each had 21 in a losing effort. Gasol also grabbed 12 rebounds.
Los Angeles was done in by 18 turnovers, seven coming in a lopsided third quarter that essentially decided the outcome.
"We're just not taking care of the ball. We get slow. We get methodical. We got stuck in the mud in the second half, and we've got to do a better job," Lakers head coach Mike D'Antoni said.
The Lakers hung around for 2 1/2 quarters, even leading by 11 at one point in the second and taking a 57-52 lead into halftime.
But Harden took over midway through the third, hitting a go-ahead 3-pointer with 6:30 remaining to start a 17-2 run, and the Rockets led from there.
Harden finished with 17 points in the quarter, and even though Howard shot just 7-of-16 from the foul line against his former club in the fourth, the Rockets never let their lead dip below double digits.
"Everybody was being aggressive [in the second half]. I just kind of played back and tried to get everybody involved," said Harden.
Ed Malloy Derrick Collins Kevin Cutler


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Listen, today is not a great day for subway news. And I know you know the subway is gross. Even when it's kind of empty and air conditioned and doesn't smell like some kind of moving, large-scale dutch oven, it's gross. All the hands that touched this pole before you, and what they touched before the pole, or maybe who sat on that seat before you if you're wearing a short skirt or shorts and jesus christ maybe let's just all stay home and agree to keep in touch via the internet.
But even that wouldn't work, because you know what you can never, ever escape? Air. And, thanks to an enterprising group of Colorado scientists, we now know pretty exactly what subway air (and street air, which is largely the same thing, apparently) is comprised of. It could all be much worse—the full report clarifies, "we encountered no organisms of public health concern," which is great—but learning about the fungi and "human skin commensal bacteria" we suck into our lungs on a daily basis could also be, well, a lot less gross. Let's explore.
Of all the random garbage floating around in the air, around 15 percent was comprised of human skin particles, and the accompanying Staphylococcus bacteria. What kind of skin, you ask? A lot if it came from pretty normal, exposed places like hands, feet, arms, and collarbones. At least some of it, though, was believed to come from the "gluteal crease," which Wikipedia helpfully clarifies as "colloquially, the 'butt crack' or 'arse crack.'" Oof.
Of the myriad "fungal sequences" found in the subway air, most of them were pretty typical, comprised of mushrooms, mildews, plant pathogens, and yeasts. Gross, but not so different from essentially everywhere else on the earth. A good 20 percent of these "had no counterparts" in the outside world, however, and there was an unusually high occurrence of "wood rot fungi," which researchers suspect is a function of wooden track structure. Rotting wooden track structure.
This is actually sort of interesting. In the midst of all the unidentifiable grime, a huge chunk of air particles were actually comprised of "genera and species characteristic of soil." As in, actual dirt. The kind things grow in! Not so bad, really.
The other side of the "normal stuff" coin here is something the study tactfully calls "environmental water." Which sounds nice and Poland Spring-y, until you think about the little puddles of filth in the tracks, inexplicably under benches, rhythmically falling from the ceiling and creating those inexplicable little perma-pustules on the platform at, say, Canal. You may be smart enough not to drink it (I hope?), but still, you're breathing it. The city itself is inside you, inside all of us. And I am maybe moving to a bio-dome.
Follow Virginia K. Smith on Twitter @vksmith.


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Modi works on ushering in 'acche din' for Varanasi
Having elected Prime Minister Narendra Modi to parliament, the ancient city of Varanasi is all set to see 'acche din' (good days) promised during the election campaign.
The Modi government has finalized an "exhaustive makeover blueprint" for the city, its spiritual and religious appeal long marred by the polluted Ganga, crumbling heritage, congested traffic and the all-pervasive filth.
Officials privy to the makeover plans told IANS that Modi recently took detailed presentations from different ministries on their proposals for Varanasi -- and approved some. The prime minister made it clear that whatever is promised should be finished within the given deadline, said a source present at the meeting.
To begin with, a statutory Master Plan is being worked upon.
The meeting in New Delhi was attended among others by Uttar Pradesh BJP president Laxmikant Bajpayi and Varanasi Mayor Ram Gopal Mohle as well as party legislators from Varanasi.
It was decided that Varanasi be developed like a metro city with a major overhaul of its arterial roads and transport system.
While poring through various proposals, Modi stressed the need to preserve the "pauranik swaroop" (ancient ethos) of Varanasi - considered the world's oldest living city now with 1.2 million people.
Other than constructing some new flyovers, the officials have been asked to work on a possible mono rail, improvement of the bus network, and a Bus Rapid Transit System (BRTS) a la the one in Ahmedabad.
The union urban development ministry has been told to rope in the Varanasi Development Authority (VDA).
The Prime Minister's Office has directed officials to ensure that water bodies in the city - there are many - like wells and ponds are re-energized and conserved as heritage.
A presentation made for Modi said the ancient landmarks and the heritage buildings would be lit up with LEDs and high power focus lights.
Riverfronts have been proposed at Varuna and Asi rivers, which for now look more like drains.
Urban Development Minister Nitin Gadkari is learnt to be spearheading the plans for Kashi -- as Varanasi has been traditionally known -- and giving critical inputs to Modi.
An exhaustive plantation drive has been planned after the widening of roads and laying an outer ring road surrounding the city.
The central government plans to do away with the overhead wiring and lay fresh, new, weather-proof underground power lines.
State BJP leader Bajpayi told IANS that he was "awe struck" at the "level of commitment Modiji" has for Varanasi, visited by millions of devout Hindus and others every year.
The urban development ministry's proposal also includes an ambitious plan of utilizing the waste water for non-potable purposes like watering plants and irrigation.
The government is pushing for setting up a plant do away with municipal solid waste.
Modi has told officials to work on the possibility of raising four satellite towns: Shivpur, Babatpur, Kashi and Sarnath.
"Other that the visual revival of the city, Modi is very keen to make the region a mini-economic powerhouse by giving it infrastructure and trading opportunities," an official told IANS.
(Mohit Dubey can be contacted at firstname.lastname@example.com)
(Posted on 26-07-2014)
Information on States of India:
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Fondling – Dark Shadows: Army of Evil
Drew tries to play this game, key word is tries.
Drew tries to play this game, key word is tries.
Ken and Justin test their driving skills.
Ken and Justin go back in time to see if 2K's football game holds up.
Jae tries to keep his pants clean for this one.
Lots of new games to discuss this week featuring eggs, robots, and death.
Xbox Live goes down as does NPD in this week's Burst.
It is time to get the stones in this classic survival horror.
Jae checks out this stylish PC title.
Drew farts around the world of South Park.
An intimate episode featuring fart magic and more.


text:
Muted response in Dubai for iPhone
The iPhone has hit stores in the UAE, with Apple penning an exclusive deal with Etisalat. But as GNTV finds out, there weren't too many takers on launch day.
Video Editor's choice
The women of the VIP Protection Unit
Dubai Police VIP Protection Unit hold a demo in Dubai to show their formidable skills
Inside India's presidential palace
An exclusive tour of India's presidential palace
wheels issue 500 teaser
A sneak peak at what to expect from wheels issue 500


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Vans Kingdom Plaid Pack
Pushing through some more releases for Spring/Summer, Vans drops a new Kingdom Plaid Pack consisting of their classic Chukka Boot model and the timeless Era. Both sneakers feature simple black/white/grey plaid print uppers accenting with white laces and midsoles. Available now at essence.
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Date: Feb 16, 2009 / Views: 7 / Author: L. Ruano


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List List Of Candydoll Model Names | Girls Room Idea
List of Victoria's Secret models - Wikipedia, the free ...
The following is a list of current and former Victoria's Secret fashion models. Bolded names indicate current or former Victoria's Secret Angels.
- Sun, 12 Oct 2014 22:00:00 GMT
Read More about List of Victoria s Secret models Wikipedia the free
List of colors: A–F - Wikipedia, the free encyclopedia
The following is a list of colors. A number of the color swatches below are taken from domain-specific naming schemes such as X11 or HTML4. RGB values are given for ...
- Mon, 13 Oct 2014 02:11:00 GMT
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List of Big Cats |Types, Names, Species - BigCatsWildCats
"Big cats" is a term commonly used in reference to large wild cats. The list of big cats below includes the different types of big cats worldwide, from tigers ...
- Sun, 12 Oct 2014 14:37:00 GMT
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List of the keyboard shortcuts that are available in ...
Some keyboard shortcuts may not work if StickyKeys is turned on in Accessibility Options. Some of the Terminal Services client shortcuts that are ...
- Sat, 11 Oct 2014 08:54:00 GMT
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Hotel Centro offers an outdoor pool making it a great business-friendly hotel choice for your stay. This 4-star Hustopece hotel offers a hair salon. If you are visiting Czech Republic for business or on vacation, make sure to take some time to relax as ...... hotel details
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Welcome to Hotel Pavlov! Enjoy a spot of lunch or an evening meal at this 3 star Pavlov hotel's restaurant where you're sure to find something to suit your taste. Outfitted with a bar/lounge, you'll find the perfect place to have a drink. Free Wi-Fi is ...... hotel details
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