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A plant-based, all-natural energy gel, Vega Sport Endurance Gel is formulated to provide instant and sustained energy plus electrolytes and vitamins during workouts.
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The products and claims made about specific products on this site have not been evaluated by Pharmaca.com or the United States Food and Drug Administration and are not approved to diagnose, treat, cure or prevent disease. The information on this site is for educational purposes only and is not intended as a substitute for advice from your physician or other health care professional or any information contained on or in any product label or packaging. You should not use the information on this site for diagnosis or treatment of any health problem or for prescription of any medication or other treatment. You should consult with a healthcare professional before starting any diet, exercise or supplementation program, before taking any medication, or if you have or suspect you might have a health problem. Pharmaca Integrative Pharmacy will not assume liability for any cross-contamination of ingredients, misprints, or adverse reactions to any ingredients in the products we carry. The products sold here may contain lead and other substances that are known to the state of California to cause cancer and birth defects and other reproductive harm. Prices and promotions are subject to change without notice. All content on this web site is copyrighted by Pharmaca Integrative Pharmacy unless otherwise stated. All online offers and prices are valid for online purchases only, and may be different from those in our retail stores. Free shipping offer may not apply to all orders and all states; see shipping policy for full details. Copyright © 2009 – 2015 by Pharmaca Integrative Pharmacy. All rights reserved.


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David Lionel Baddiel (born 28 May 1964) is an English comedian, novelist and television presenter.
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The Korg line of compact, portable synthesizers feature compelling instruments highlighted by brilliant sounds and intuitive expression, powered by Korg's powerful synthesis engines. During practice sessions or live performances, musicians can feel this amazing power at their fingertips -- whether calling up sound programs and combinations, or simply tweaking away through various parameters and algorithms. Check out Kraft Music's selection of advanced synths, keyboard synthesizers, music synthesizers and MIDI synths that all carry the proud Korg pedigree.
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StoryCorps is partnering with KUNV 91.5 "The Source" and the City of Las Vegas Office of Cultural Affairs to record, preserve, and share the stories of communities in Las Vegas, NV from April 4, 2013 – April 27, 2013.
The MobileBooth will be located at:
Centennial Plaza
401 South 4th Street
Las Vegas, NV
Reservations open on Mar 15, 2013 at 10 AM local time. We expect a high volume of calls and reservations online. To assure you have an opportunity to make a reservation, we encourage you to call 800-850-4406 or make a reservation online as soon as they become available.
Si desea hacer una reservación en español o necesita más información, por favor llame al 800-850-4406, las 24 horas al día, los 7 días de la semana.
Call our 24 hours a day, 7 days a week, Spanish/English reservations line at 800-850-4406.
Go to our reservation system by clicking on the "Reserve now" button above.
- Log in to our reservation system. First-time visitors can create a free account.
- Find a day and time that works for you and your interview partner. Please remember, all participants must be 10 years or older. If there are no times available, please keep checking back in case of cancellations. You will only have to register the first time, so to check back you simply sign in and look for open reservations.
- Pledge a donation. StoryCorps is a free public service, but we do ask for donations to help cover our costs and keep StoryCorps accessible to all. We suggest $25, but you can contribute as much or as little as you wish. Donations are processed on-site after your interview.
- Come to StoryCorps and have the conversation of a lifetime!
Parking & Directions
Parking for StoryCorps participants will be located at Historic 5th Street School in Downtown Las Vegas on the corner of South 4th Street and East Clark Avenue. Parking spots will be marked with orange cones.
Wait List
If there are no appointments available, we suggest adding your name to the Wait List. The Wait List is available to everyone on a first come, first serve basis by calling 800-850-4406. You may add your name to the wait list for the duration of the current reservation openings. We will contact you by phone if an appointment becomes available. If you are not able to take the appointment, you can still remain on the Wait List. We will go down the Wait List until we reach someone who can take the open reservation.
Cancellation Policy
StoryCorps is a public service intended to serve as many people as possible. We strive to make this happen daily. We understand cancellations are necessary sometimes. We ask that you cancel your appointment in a timely way in order to ensure StoryCorps can continue to operate in so many cities and offer these highly coveted times to someone on our waiting list. We appreciate your commitment to StoryCorps by reading and following these policies. Cancellation and Credit Card Policy At the time you register for an appointment, you will be asked for your credit card information. If you cancel your appointment within 48 hours, you will be charged a $50 cancellation fee. This credit card will not be used to process any donations after your interview. If you do not have a credit card, you can still sign-up for an interview by calling 800-850-4406.


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I'm looking to make some cuts in a 55 gallon oil drum and I'm trying to avoid getting blown up.
First of all the drum is closed end with two bungs (one large, one small). From what I can tell it had almond oil in it before I picked it up. There is some remaining oil in the bottom.
I want to cut a door in the side of the drum and turn it into a bbq. I'm probably going to use abrasive cutoff disks in my angle grinder so there will be a lot of sparks and I'm worried about igniting the oil in a confined space.
Does anyone have any advice for cleaning the barrel out or any other suggestions for how I should go about doing this? Thanks.
Results 1 to 10 of 64
Thread: Oil drum cutting
Oil drum cutting
Does anyone know what the flash point of almond oil is?
Once you figure out if the oil is flammable, then we can tell you better how to do it. I really don't know if it is flammable or what the risk of explosion may be.
When in doubt, don't do it.
Look for alternate methods such as a sawsall or a jig saw.
Sorry I can't be more help but I know nothing about the properties of almond oil and I'm not willing to take a guess when there is a risk of injury involved. DaveIf necessity is the Mother of Invention, I must be the Father of Desperation!
John Blewett III 10-22-73 to 8-16-07
Another racing great gone but not to be forgotten.http://video.google.com/videoplay?do...modified&hl=en
02-22-2008, 04:50 PM #3Member
- Join Date
- Oct 2006
- Location
- Cleveland, Ohio
- Posts
- 38
I'd empty it and steam it out if I could. If you can't steam it out, Dawn dish soap is a good oil cutter.
Another option is to take it to a car or truck wash and have them clean it out for you.
You could also purge it with Co2 or another inert gas (Argon may be kinda expensive though).
I wouldn't use car exhaust due to the possibility of unburned fuel.
Clean it 'till you feel safe cutting it.
Will steaming work on a heavy oil like this? I'd imagine the idea of steaming it is to drive out the volatiles.
I poured a little of the excess oil out and it is quite a bit like olive or canola oil. It won't light on fire by itself but when I soaked a napkin in it it burned. It doesn't seem very volatile. From the MSDS the flash point is >400F.
I think I might try the dish detergent idea.
Thanks for the suggestions.
Here is an MSDS sheet for Almond Oil. It has a flash point of 400 degrees so I dont think you want to torch it.
What else is there besides welding and riding. Besides that
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i just cut a 10w30 55gal drum like that. used my plasma cutter. i just filled it up with water and made the cut, no problem. lay it on its side so the big hole is up and fill it to the cut point and cut.
well it worked ok for me any way. although it may not be the best option. but cold oil, cold water, hot plasma cut, no problem. dish soap will take enough out to not have a problem i suspect. that stuff cuts oil great.thanks for the help
hope i helped
feel free to shoot me an e-mail direct i have time to chat. firstname.lastname@example.com
summer is here, plant a tree. if you don't have space or time to plant one sponsor some one else to plant one for you. a tree is an investment in our planet, help it out.
02-23-2008, 07:59 PM #7Member
- Join Date
- Oct 2007
- Posts
- 41
Just for the uninitiated in the group- I had a barrel that had solvent in it, knew it was flammable. Left it sit outside both bungs open, protected from weather. Sat a year, poured in about 1 1/2 gallon of water in it. Cut off 1/2 of top with stick welder. No smell or fumes cut remaining half off, when I got to about 1/2" from end heard a sound like sparklers going off, lifted helmet and seen sparks shooting out like a Roman candle, Thinking thats COOL, then barrel gave a big POOF and stopped sparking, just had a restroom break so being "empty" was a good thing. Problem? Pile of dust in barrel and with cutting and hot metal falling in dried up the water enough to start chain reaction. Next time more water.
Even when you think it's O.K., it just might not be. I have cut many a barrel and propane tanks open and know many safe ways to do it. I was not going to give someone unexperienced advice on how to do it. You happen to be lucky things didn't go much worse for you.
I will say it again, If you are not absolutely sure of what you are doing, don't do it. Leave it for some one who does.If necessity is the Mother of Invention, I must be the Father of Desperation!
John Blewett III 10-22-73 to 8-16-07
Another racing great gone but not to be forgotten.http://video.google.com/videoplay?do...modified&hl=en
02-24-2008, 02:36 AM #9Member
- Join Date
- Oct 2007
- Location
- stationed in bamberg germay
- Posts
- 64
the tc 9-237 has a section on cleaning fuel tanks for repair might give some ideas
The proper way to cut open a drum without any chance of injury is to find some numbnut that just got a plasma cutter and ask him to impress you.Nick
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Home > Outdoor Living > Weather Instruments > Home Weather Stations > DX Deluxe Projection Weather Station
DX Deluxe Projection Weather Station
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Learn Technology with Lynda.comWritten by Amanda G. on 06/10/2014
Since October 5, 2009 Darien Library has offered lynda.com as a technology education resources. The service is available to residents, those who work full-time in Darien, and non-resident Contributors to the Library at the $300 annual level, lynda.com currently provides 1,612 online subscribers with instruction in a wide range of Mac and PC software programs.
With Lynda, you can set your own training schedule. The site includes practice exercises and a helpful feature that keeps track of where you left off each time you end a session. In addition, upon completion of the video training, you can take part in lynda.com's Certificate of Completion program.
How to Gain Access
Come to the Help Desk in the Power Library (aka computer room) on the Lower Level or call the Help Desk at 203-669-5238 to get started. Give us your name and email address so we can register your account. You will receive an email from lynda.com to active your account. We will then assign you to either an in-library access for a couple hours or you can access lynda.com from home for seven days. If no one is waiting, you can ask for your access to be renewed.


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|CrossFit 580
|Markian Kaczaj
|3987 First St. Suite Q
Livermore, CA 94551
Like, Tweet or +1 CrossFit 580
WOD: 8/31/15 Power Clean 2-2-2-2-2-2-2 reps 21 – 15 – 9 Power Clean 135/95 Front Rack Lunge Push Jerk I get excited every single day when I see more and more women falling in love with lifting weights. It seems that women are starting to no longer be obsessed with what their bodies look like but hav…
A. Three sets not for time 6-8 Deadlift @ 41×1 8-10 DB Box Step-Up's 20 Unbroken Russian KB Swings B. EMOM for 24min 1st: 5 Hang Power Snatch 2nd: 10 BB Front Rack Reverse Lunges 3rd: 9 Toes...
8/31/15 The. Matt. Baumann! Strength Front Squat (7 x 1) 85-90%, same weight for all sets. 500 m Row for every missed rep. Conditioining 7 min AMRAP: 5 Push Presses 5 Strict Pullups L1: 135/95, 2 Muscle Ups L2: 115/75 L3: 95/65, Banded http://crossfitevviva.com/2015/08/30/83115
8/31/15 The. Matt. Baumann! Strength Front Squat (7 x 1) 85-90%, same weight for all sets. 500 m Row for every missed rep. Conditioining 7 min AMRAP: 5 Push Presses 5 Strict Pullups L1: 135/95, 2 M...
In case you missed it!
Don't forget 9:30 Sunday class tomorrow! A great chance to get one more WOD in before the week is over :) See ya there - Coach T
Skill: Muscle up drills (15 min) 2sets -Swing Hip to Rings x5 -Ring Supports x5 -Transitions x5 -Jumping MU transition (no dip) (no dip) x5 WOD 1: EMOM 10 10 wallballs 1-3 MU WOD 2: For time 20 T2B 50 Plate Burpees (45/25) 20 T2B (12 min cap) Sasha Yevelev is the CFPH Athlete of the Month for August! [ 42 more words. ] http://crossfitph.com/2015/08/29/wod-sunday-aug-30/
Skill: Muscle up drills (15 min) 2sets -Swing Hip to Rings x5 -Ring Supports x5 -Transitions x5 -Jumping MU transition (no dip) (no dip) x5 WOD 1: EMOM 10 10 wallballs 1-3 MU WOD 2: For time 20 T2B 50 Plate Burpees (45/25) 20 T2B (12 min cap) Sasha Yevelev is the CFPH Athlete of…
Huge congrats to Mangela for wining the best #4# Bench Press with an easy 116 press at 108# BW. She would have gone heavier but squeezed in 2 rushed attempts in between the male dominated lifting platform. Also hats off to Marques Morgan who smashed a 400 bench at 206# then went on to unofficially lift up to 425. Over 2x BW on the bench press is insanely strong.
Fyi: Toilets are fixed! Tree roots vs sewer pipe = sad CFO'ers Giant THANK YOU to Mini for overseeing repair and cleaning up (ew!).
We have one athlete in first for scaled and one in second for men's masters before the last WOD! Stay tuned...
TODAY'S PARTNER WOD In 7 minutes: Establish a Thruster 3 rm (from the rack) 2 minute rest Then, AMRAP 7 minutes: A. 5 hspu 15 ttb B. 5 hang power clean (155/105) 40 double-unders *one partner starts on A and the other on B. Once the 7 minutes is up, the partners switch and continue on for another 7 minutes (14 minutes total). Score is total number of rounds/rep + thruster total. http://americanrivercrossfit.com/08-29-2015/
TODAY'S PARTNER WOD In 7 minutes: Establish a Thruster 3 rm (from the rack) 2 minute rest Then, AMRAP 7 minutes: A. 5 hspu 15 ttb B. 5 hang power clean (155/105) 40 double-unders *one partner starts on A and the other on B. Once the 7 minutes is up, the partners switch and continue …
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Active outdoor day… CrossFit Team WOD: 10 Rounds 20 Wallballs (20/14) 10 Pull ups 200m Run
Bring a friend, or your spouse, or your girlfriend, or boyfriend, or that special someone you'd love to be your girlfriend or boyfriend...or...Read more...
WOD 08/29 Hey CFE'rs, So we have been thrown a bit of a wrench. Both Rocklin and Roseville schools have delayed the earliest drop off times for the '15-'16 school year. With our kiddos headed back to school this week, we are addressing some concerns about being able to get to class after the drop off routine. We will be pushing the start of the mid morning class back to 845 as of August 31st. Saturday's Focus: Something EXTRA Special from Coach Beth! The post WOD 08/29 appeared first on Crossfit Echelon.
True story Saturday 8/29/15 "Pike" 5 rounds for time: 20 Thrusters (75/55) 10 Strict dips 20 Pushups 10 Strict handstand pushups 50m bear crawl Why am I doing this? Age is irrelevant when it comes to fitness
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SATURDAY WOD August 29, 2015
Nineteen members of the Granite Mountain Hotshots firefighting team tragically lost their lives on June 30, 2013 while fighting a fire in Yarnell, AZ. Here is where you can make a donation to support the families of the lost heroes. Your support will be collected by the CrossFit Foundation where 100…
Support Courtney Walker! Go watch the Grid League! https://fire.npgltickets.com/order/group/walker/
Don't miss this California rivalry match as your SF Fire take on visiting LA Reign in an action packed night of GRID. General Admission and two rows of GridSide seating will be available. Individual seats will be on a first come first serve basis.
Over Head Squat x3 WOD 5-3-1 Bar Muscle Up Dead Lift (315/225)
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If you missed Hawaii News Now Jennifer Robbin's "On the Radar" at CrossFit 808, here's the link to the story: http://www.hawaiinewsnow.com/story/29896510/on-the-radar-cross-fit-808
Cross Fit is an obsession for some people. One of our local cross fit teams from Kalihi did really well in a recent international competition. Our Jennifer Robbins visited Cross Fit 808 in this edi...
Don't forget - we are hiking the Manitou Incline TOMORROW! Meet at the gym at 6:30AM, we will be leaving as a group at 7:00.
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Snatch 3/12: 55/3, 65/3, 75/3, (4x) Buy In..EMOM/4 (1) Press <90% Tabata (alternate) :20/:10 x 16 D/L (185/123) & Thrusters (75/53)
The workout for 8/28 is... Pull Ups/Dips: 5-5-4-3-2 Tabata: Russian KB Swings (70/53). Rest 2 min then Tabata: Double Unders. Count total reps for each WOD - 5 Rounds for QUALITY: - 10 Kipping Swings (adv: on rings or muscle up transitions) - 5 Candlesticks - 10 Hollow Hold to Arch/Superman Rolls Jeopardy! Category: Documentaries Answer: Michael Moore's documentary about the American health care industry had this five-letter title
Be sure to check out today's #CrossFit #WOD on the I AM CrossFit site! http://iamcrossfit.com/
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Event 4: for time with a 16 min time cap (1st event of day 2) 800m run OHS x 20 – 105/75 800m run C&J ladder: 9 X 135/95 7 x 155/115 5 x 185/135 3 x 205/155 Teens 45-49 (OHS 85/55) 105/75 – 115/85 – 135/95 – 155/115 50+: (OHS 65/35) – 95/65 – ...


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false pdp {"scheme":"product1_cr"}
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Rated 4.4 out of 5 by 59 reviewers.
Rated 2 out of 5 by mdw6 Very thin fabric The fit of this dress was great, and I loved the cut -- unfortunately, the fabric was rather lacking. Even with a black bra on, it was clear where the lines were, and not because it was too tight. And despite it being a summer-weight dress and a dark color, I definitely felt I would always need to wear a slip under it. I thought it was exactly what I was looking for, but I guess I'll keep looking. July 25, 2015
Rated 4 out of 5 by KayRay Great for travel I have had this dress for several years, and find it to be the perfect LBD for travel. We cruise several times a year, and this is the perfect dress for cocktail hour, dinner in a specialty restaurant, or a casual night onboard. It always comes from the luggage unwrinkled and ready to wear. I wear it with the black/white shrug shown in the photograph, or a pashmina, belted or not. It is just as useful at home for dinner out or the theatre, or even a week in NYC. March 24, 2015
Rated 5 out of 5 by natesnana great travel dress Bought for cruise. Ordered MP for my 5'3" 126 lb frame, looks great on, not too tight, just the right length. I rolled it up and the wrinkles fell out when I hung it up. Washed in sink, hung to dry, and touched it up with a steamer when needed. Wore with jacket, scarf, or jewelry to change the look. Many compliments, now will also wear to two summer weddings. Wish it came in other colors like blue, purple, or jade, would definitely buy another one. February 8, 2015
Rated 5 out of 5 by BlueIce Just what I was looking for! This dress is flattering, and travels very well. It does run a bit longer than the picture would make you think, but it's still versatile as well as comfortable and useful. Fabric reminds me of something Chico's used to carry. February 1, 2015
Rated 5 out of 5 by MandMsTutu Just what I was looking for! I bought this for a trip to Europe this fall. I was looking for something that would pack easily, shed wrinkles and could be dressed up with accessories. This seems to fit the bill. It does show any extra bulges I might have but not as much as I would have thought. Am looking forward to trying it out in September! July 31, 2014
Rated 5 out of 5 by grace2u elegant and comfortable After looking at a number of bridal shops, I bought the dress and jacket shown with it for my son's wedding in September. Elegant and comfortable and will be perfect. Much more 'me' than dowager brocade dresses covered with sequins. The jacket did not disappoint either. I was surprised at the softness of the material; thought it may be stiff from looking at the picture. I will also be able to wear the dress to various occasions, and change it up with different jackets, scarves and sweaters. And no wrinkles. It was well worth the money. I'm sure to wear it many times. Would love more colors like navy and a deep chocolate brown. July 31, 2014
Rated 5 out of 5 by Mehg Great multi- occasion dress Fits well. Looks great. Will use it for travel constantly. July 30, 2014
Rated 3 out of 5 by Lyonness Too warm for summer I hoped to use this dress for casual summer events, but the fabric is heavy and the L size, which is often perfect for me, was too clingy in the mid-section, showing more bulges than I like. I think women who are very tall would look better in this style. Also I think a long slit up the side is unnecessary because the fabric is very stretchy. I sent it back and decided I just can't find a well fitting dress for my body shape at Travelsmith. July 21, 2014
Product:35258-Tres Elegant Knit Tank Travel Dress


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Robert Francis (born September 25, 1987 in Los Angeles, California) is a multi-instrumentalist, Americana singer-songwriter. His debut full-length album One By One was released in August 2007 by Aeronaut Records. The record has gained attention due, in part, to Francis''s musical performances that stem from his "eccentric classical-record-producer and pianist father, a Mexican mother who frequently invited her sisters over to sing Ranchero songs, a Hari Krishna brother, and two older sisters who were in various rock bands that played in clubs all over the city". His sound is distinguished by his "bright, gravelly baritone", often conveying the emotion of a more "hardened performer". One of his popular songs, "Junebug", was featured as a free song on iTunes for a week. This song reached #26 in Germany and #22 in Switzerland.
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Interpretive Handbook
7807 :
Coagulation Factor V Inhibitor Screen, Plasma
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Factor V inhibitors can occur in patients with congenital factor V deficiency after transfusion of fresh frozen plasma, however, more commonly, they occur spontaneously in previously healthy older patients who have no underlying diseases. Topical bovine thrombin or fibrin glue, which contain bovine thrombin and factor V, are commonly used in surgery for topical hemostasis, can result in development of anti-bovine thrombin/factor V inhibitors that cross-react with human thrombin and factor V. Other associations include antibiotics, transfusions and malignancies.
Useful For Suggests clinical disorders or settings where the test may be helpful
Detection and quantitation of inhibitors against coagulation factor V
Interpretation Provides information to assist in interpretation of the test results
Normally, there is no inhibitor, ie, negative.
If the screening assays indicate the presence of an inhibitor, it will be quantitated and reported in Bethesda (or equivalent) units.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
If the presence or type of inhibitor is unknown, LUPPR / Lupus Anticoagulant Profile should be ordered, except for screening studies in patients with known hemophilia A or B.
Occasionally, a potent lupus-like anticoagulant may cause false-positive testing for a specific factor inhibitor (eg, factor VIII or IX); see preceding Caution statement.
Not useful for the detection of a lupus-like circulating anticoagulant inhibitor or other inhibitors not specific for coagulation factors.
Not useful for the detection of a non specific circulating anticoagulant.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Adults: 75-165%
Normal, full-term newborn infants may have borderline low or mildly decreased levels (> or =30-35%) which reach adult levels within 21 days postnatal.*
Healthy premature infants (30-36 weeks gestation) may have borderline low or mildly decreased levels.*
*See Pediatric Hemostasis References in Coagulation Studies in Special Instructions.
Clinical References Provides recommendations for further in-depth reading of a clinical nature
1. Feinstein DI: Acquired inhibitors of blood coagulation. In Hematology: Basic Principles and Practice. Edited by R Hoffman, EJ Benz Jr, SJ Shattil, et al. New York, Livingstone Press, 1991, pp 1380-1394
2. Kasper CK: Treatment of factor VIII inhibitors. Prog Hemost Thromb 1989;9:57-86


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Whether you love or hate the term It Girl, Jennifer Missoni is definitely a girl about town who has, by all accounts of the definition, it: amazing style, an acting career on the rise, a lovely fiancé, oh, and a famous-beyond-famous last name. After meeting up with her for an exclusive photoshoot, though, we were thrilled to find out that while she's a red-carpet stunner, in her everyday life, Jennifer's style reflects her casual-cool expat upbringing.She favors comfort over shock-factor, relying heavily on workout clothes while bustling to and from her Tribeca apartment (yeah, we hear you, with the collective "holla-back"), and opts for dirty hair over shampooing every day (again, we can relate). We guess the difference, then, between It Girls and this girl, is that we're totally and truly rooting for her. After having seen Ms. Missoni's recurring roles on shows like Gossip Girl , we so look forward to watching her big screen debut, opposite Christian Slater (eek!) in March's Playback. And as for the fashion stuff? Dressing may come naturally to Jennifer (it is, after all, in her blood) but she'll leave the designing to the fam, in favor of taking director's cues from the pros. And we're cool with that. Click through to check out four of Jennifer's favorite winter looks, and to steal a few of her styling tricks.
Photographed by Sam Horine


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Kathryn Oostdik, Martin Ensenberger, Benjamin Krenke, Cynthia Sprecher and Douglas Storts
Promega Corporation
Time and labor savings continue to be key goals for database and paternity labs. Increasing sample numbers and tightening government budgets necessitate that database laboratories seek process improvements. Similarly, efficiency gains are key for commercial paternity testing organizations to remain competitive.
Recently, the STR-typing community has begun to adopt the concept of "direct amplification" from unpurified samples on FTA® cards. Significant time and labor savings can be achieved by eliminating DNA purification and amplifying samples directly from buccal or blood samples on FTA® cards. Another time-consuming process is the PCR itself—often three hours or more. The duration of this step often dictates daily workflow for laboratories, and when combined with DNA purification and capillary electrophoresis, the process often requires more than one day. Although thermal cycling is a hands-off step, decreasing sample time on an instrument with rapid amplification allows samples to be processed in a single day.
The PowerPlex® 18D System allows direct amplification and reduced thermal cycling time to provide a simpler and faster genotyping system for database and paternity laboratories. Additionally, the 18 loci amplified represent all markers commonly typed in CODIS laboratories.
"Significant time and labor savings are achieved through robust direct amplification and rapid PCR."
"Significant time and labor savings are achieved through robust direct amplification and rapid PCR."
The PowerPlex® 18D System (Cat.# DC1802 and DC1808) was developed for direct amplification of buccal or blood samples on FTA® cards. The five-color system (Figure 1) contains primers for amplification and genotyping of 17 STR loci (D2S1338, D19S433, Penta E, Penta D, D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX and FGA) as well as Amelogenin, encompassing all 13 CODIS loci. One primer for each locus is labeled with fluorescein (blue), JOE (green), TMR-ET (yellow) or CXR-ET (red). Detection is performed with the Applied Biosystems 3100, 3130 or 3500 Genetic Analyzer. Amplified samples can be sized with the supplied CC5-labeled Internal Lane Standard 500 (CC5 ILS 500). Proper color deconvolution is achieved by matrix standardization or spectral calibration using the PowerPlex® 5-Dye Matrix Standards (Cat.# DG4700). Finally, a new control DNA, 2800M, is provided in the kit (Figure 2). This DNA is derived from a single-source, human blood sample.
The PowerPlex® 18D System contains all 13 CODIS loci: D3S1358, THO1, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX and FGA, plus Amelogenin, Penta E, Penta D, D19S433 and D2S1338.
Five nanograms of 2800M Control DNA was amplified for 27 cycles on a GeneAmp® PCR System 9700 using the PowerPlex® 18D System. Samples were analyzed using a 3kV, 5-second injection and a 1,500-second run time on an Applied Biosystems 3130xl Genetic Analyzer.
While the PowerPlex® 18D System can be used to amplify extracted samples, such as purified reference samples, the kit was optimized for direct amplification from samples on FTA® cards. Components in FTA® cards lyse cells contained in the deposited sample and promote DNA accessibility for PCR. However, components that drive lysis can inhibit PCR; thus, washing or purification protocols are generally incorporated prior to amplification to type samples on FTA® cards successfully. This is not necessary with PowerPlex® 18D. Core punches are taken from sample-spotted FTA® cards and placed directly into the PowerPlex® 18D reaction. The master mix in the PowerPlex® 18D System overcomes inhibitors without washing or purification (Figures 3 and 4).
A buccal sample was collected and immediately transferred to FTA® paper. Reactions were set up using one (Panel A) or two (Panel B) 1.2mm punches from the same card. Amplifications were performed using 27 cycles on a GeneAmp® PCR system 9700. One microliter of amplified sample was analyzed using a 3kV, 3-second injection and 1,500-second run time with the G5 dye set on an Applied Biosystems 3130xl Genetic Analyzer.
Whole blood (125µl of K2 EDTA-anti-coagulated blood) was spotted onto FTA® paper. Reactions were set up using one 1.2mm punch and amplified using 27 cycles on a GeneAmp® PCR system 9700. One microliter of amplified sample was run with a 3kV, 3-second injection and 1,500-second run time with the G5 dye set on the Applied Biosystems 3130xl Genetic Analyzer.
With direct amplification, sampling can affect results more than with standard amplifications. Although a typical 1.2mm punch contains sufficient material for successful amplification, the distribution of sample across an FTA® card varies, most notably with buccal samples. Indicating FTA® cards help locate a buccal sample on the card and, therefore, the ideal area to punch. However, indicating cards only show where the surface was wetted. Wetted but bare areas of the card could exist, and sampling from those areas will not result in amplification. Failures due to bare punches can be resolved by including two punches per reaction, increasing the probability that the reaction contains at least one sample-covered punch and increasing first-pass success rates. As shown in Figure 5, only a slight increase in peak height may be seen for reactions with two punches compared to those with one punch. However, the PowerPlex® 18D System can accommodate two buccal punches. For blood samples on FTA® cards, one punch is recommended. The use of two blood punches is not necessary, as sample distribution is clearly visible and generally more consistent than buccal punches (Figure 5). In many cases, the use of two FTA® punches when working with blood on FTA® cards can actually result in poorer results due to an increase in PCR inhibitors associated with blood samples on FTA® paper.
FTA® cards from two buccal donors (Panel A) and two blood donors (Panel B) were amplified using one or two punches per reaction, with three replicates each. The average locus peak height of three replicates for each condition is shown.
Previous commercial STR kits were optimized for older thermal cycling technology and performance of the enzyme available at the time. Cycle times generally approach or exceed 3 hours (Figure 6). Consequently, processing each plate of samples from sample to analysis, including extraction, cycling and capillary electrophoresis (CE) analysis, requires more than one day. Thermal cycling time for the PowerPlex® 18D System is less than an hour and a half using a simple, two-step cycling program (Figure 6). With direct amplification, samples that are punched in the morning are immediately ready for PCR. Less than 2 hours later, those same samples are ready to be placed on the CE instrument. This simplified process reduces total hands-on time as well as the number of manipulations required, saving staff resources and reducing the chance of error.
The approximately 3 hour, 3-step cycling program for PowerPlex® 16 HS System is shown (left) and compared to the approximately 80-minute, two-step cycling program for PowerPlex® 18D System (right).
The PowerPlex® 18D System was developed to simplify and accelerate STR typing for database and paternity testing. With 18 loci the system is compatible with existing databases, allowing maximum data sharing between CODIS laboratories and future results to build on those databases. In addition to significant time and labor savings through robust direct amplification and rapid PCR, the PowerPlex® 18D System has the flexibility and performance to accommodate the sample types currently submitted to laboratories and successfully meet laboratory goals. Consequently, the PowerPlex® 18D System is an effective option to increase lab efficiency and simplify sample process workflow.
Scientific Style and Format, 7th edition, 2006
Oostdik, K. et al.
The PowerPlex® 18D System: A Direct-Amplification STR System with Reduced Thermal Cycling Time.
[Internet] 2011. [cited: year, month, date]. Available from:
American Medical Association, Manual of Style, 10th edition, 2007
Oostdik, K. et al.
The PowerPlex® 18D System: A Direct-Amplification STR System with Reduced Thermal Cycling Time.
Promega Corporation Web site.
Updated 2011.
Accessed Month Day, Year.
Contribution of an article to Profiles in DNA does not constitute an endorsement of Promega products.
PowerPlex is a registered trademark of Promega Corporation.
FTA is a registered trademark of Flinders Technologies, Pty, Ltd., and is licensed to Whatman. GeneAmp is a registered trademark of Roche Molecular Systems, Inc.
Products may be covered by pending or issued patents or may have certain limitations. More information.
All prices and specifications are subject to change without prior notice.
Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.


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Fellas, stop putting it off. When it comes to your wardrobe, it's officially spring-cleaning time: You need to haul your sweaters to the dry cleaners, zip those heavy coats into garment bags, and retire the winter-weary boots. For the fellas, here's where we come in: We scoured the streets and the web to pull together an exhaustive, game-changing list of season-ready wardrobe essentials.
Whether you're a dude in need of a closet overhaul or a lady looking to revamp the man in your life, we've got plenty of staple wear-with-alls, plus some on-trend curveballs for the more fashion forward. The options — dudes, we know you love 'em! — don't stop there: With high and low price points for each of the 30 styles on offer, you're free to splurge or save. Procrastination time is officially over, boys.


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Ryan White and Gay Men's HIV/AIDS Awareness Day
Ryan White and Gay Men's HIV/AIDS Awareness Day
As it was in the earliest days of the HIV/AIDS epidemic, gay and bisexual men comprise the majority of HIV/AIDS cases in the U.S., representing over half of those infected with HIV/AIDS.
About two-thirds of new HIV infections in the U.S. each year are among gay and bisexual men, sometimes termed men who have sex with men (MSM). The impact is heaviest on African American MSM. Get the latest facts on HIV/AIDS and MSM.
National Gay Men's HIV/AIDS Awareness Day is September 27, 2014. Awareness day activities focus on testing and prevention, engagement in care for those who are infected, and battling stigma and homophobia.
Black MSM Retention in Care Initiative
In mid 2014, HRSA/HAB launched an HIV prevention and care resource/technical assistance center for HIV care providers focused on strengthening engagement and retention in HIV clinical care among young and adult Black gay, bisexual, and other men who have sex with men (MSM).
Learn more about Center for Engaging Black MSM Across the Care Continuum (CEBACC), a HRSA cooperative agreement with NASTAD, the National Alliance of State and Territorial AIDS Directors.
More TA and Training Resources on MSM
See the TARGET Center topic page on men who have sex with men. Highlights:
- Prevention and Management of Sexually Transmitted Diseases in Men who Have Sex with Men: A Toolkit for Clinicians
- Engaging Hard-to-Reach Populations: Empowering the Patient (iHIP webcast)
- Engaging Young MSM of Color in HIV Care (in+care webcast)
- Nurses Health Education About LGBT Elders (HEALE) Curriculum
- LGBT Webinar Series


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The National Academies of Sciences, Engineering, and Medicine
500 Fifth St. N.W. | Washington, D.C. 20001
Copyright © National Academy of Sciences. All rights reserved.
Terms of Use and Privacy Statement
"Executive Summary."
Putting People on the Map: Protecting Confidentiality with Linked Social-Spatial Data.
Washington, DC: The National Academies Press, 2007.
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Loras College will host the Rivers as Bridges Family Friendship dinner on Wednesday, July 25, 2012 at 6:30 pm in the Alumni Campus Center Café. Loras College will host Chinese High school students and their chaperones on a tour of the college followed by a dinner in the Loras College Café. Loras students and faculty will network, share cultural experiences and build relations as they participate this new initiative. A delegation of Loras College's international students will also share their experiences related to attending Loras, living in Dubuque and acclimating to life in the United States. Loras graduate Matt Moroney ('91), Deputy Secretary, Wisconsin Department of Natural Resources will be in attendance at the dinner.
The program was described as "a project with a great deal of promise and imagination" by former Secretary of State Lawrence Eagleburger before he died last year. It also responds to Chinese Vice President Xi Jinping's call for innovative, people-to-people projects that emphasize mutual respect and win-win initiatives. Mr. Xi visited Muscatine, IA in February and made his comments to its residents who visited China in June.
"We are excited to be part of history in connecting the people of our countries, using rivers as bridges," said Xiaojun Lu, president of the Environment and Public Health Network for Chinese Students and Scholars, which is sponsoring the nearly three-week event along with the Iowa and Wisconsin Departments of Natural Resources.
The educational program is called Rivers as Bridges. Students will learn scientific procedures in field research, wet labs, and hands-on experiments in natural resources sciences with study about air, water, biology, soil, fisheries, wildlife and wetlands.
The students will tour colleges and universities, camp, fish, swim and do service projects in Illinois, Iowa and Wisconsin. They will meet US students, attend a family friendship dinner and share culture with Ho Chunk youth and then join them at a water park. The students will make posters on science and friendship and earn a recommended credit from the Northland Pines High School in Eagle River, WI.
A Rivers as Bridges goal, Lu said, is to begin a youth-inspired relationship that can result in a renewal of the 1972 Shanghai Communiqué. That diplomatic document, signed by Premier Zhou Enlai and President Richard Nixon sought normalization of relations. The Rivers as Bridges concept and link to the Communique came from a 2010 "campfire diplomacy" college student event in Iowa and Wisconsin called the River Spirit Exchange.
Stops include Evanston, Chicago, Moline and Rock Island in Illinois; Davenport, Muscatine, Fairport, Pleasant Valley, Bellevue and Dubuque in Iowa; Platteville, Mineral Point, Madison, Columbus, Horicon, Ripon, Baraboo, Wisconsin Dells, Rhinelander, Eagle River, Appleton and Milwaukee in Wisconsin.


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Adrian Sutil says he will "probably" have another season at Force India although "no decision" has been made...
Adrian Sutil says he will "probably" have another season at Force India although "no decision" has been made.
Having taken a year off due to his personal troubles, Sutil returned to Force India this season.
The German made a flying start, beating his team-mate Paul di Resta to seventh at the Australian GP. That, though, is one of only six top-ten results Sutil has achieved.
But despite being outscored by di Resta by 10 points, Sutil reckons he'll remain at Force India next season.
He told Autosport: "I will probably do another season here.
"It's a good team. At the moment, we are not as strong as we used to be but it is one of the strongest private teams and it's hard to find a better seat so I would like to do another season here.
"But I haven't made up my mind completely and no decision has been made."
The 30-year-old was quick to point out that the decision is not solely Force India, it also depends on whether he wants to stay put.
"It's not all about the team and whether they say I am allowed to drive or not, I can also make some comments [about] if I want to stay here.
"It's 50/50, it's not that they decide what I'm allowed to do.
"I think I am in a strong position, I have done my job this year and you would have to find someone else who does the job like I do.
"If there is a better drive somewhere out there and they want him, fine take him and good luck."


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Daily Archives: March 23, 2015
Volunteers Sought to Judge Leadership in Action Fair at Robert O. Gibson Middle School
March 23, 2015 | Category: News | Topics: Community Service, Law Related EducationOn April 16, 2015, Nevada attorneys are needed to judge the students who will participate in the Leadership in Action (LIA) Fair at the Robert O. Gibson Middle School in Las Vegas. The LIA Fair begins at 5:30 p.m. and will be held on the school's campus at 3900 W. Washington Avenue, Las Vegas, Nevada (Decatur/95). The dual language... Read more
Pat Getter to Present Depo Tips at CLE Seminar for Lawyers – Feb. 5, 2015
January 23, 2015 | Category: News | Topics: CLE Seminar, trial preparationOn Thursday, February 5, 2015 at Noon the Clark County Bar Association will host an hour-long class of continued legal education for attorneys to learn how to better prepare deponents during trial preparation. The CLE seminar "Deposition vs. Root Canal: Preparation Techniques to Make Dental Work the Only Painful Experience" will feature presenter Pat Getter, a member of the... Read more


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|News » Archives
Recap | BoxscoreBucs rally for 5-4 win over Broncos in game one of doubleheader
Saturday, February 26, 2011
JOHNSON CITY, Tenn. (Feb. 26, 2011) - The ETSU baseball team overcame a 4-1 deficit for the second straight game to pick up a 5-4 comeback win against Rider Saturday afternoon in game one of a doubleheader at Cardinal Park.
Senior Ben Booker (Knoxville, Tenn.) picked up his second win of the season throwing 3.0 scoreless innings of relief for the Buccaneers (4-1).
In the top of the first, the Broncos (0-2) went in front 1-0 after Karl Johnston came in to score from third on a ball hit to short by A.J Albee.
The Bucs answered in the bottom half as centerfielder Matthew Scruggs (Knoxville, Tenn.) led off the inning with a triple to deep center. A couple batters later, Dylan Pratt (Johnson City, Tenn.) brought Scruggs home on a slow grounder to third to even the score at 1-all.
Rider regained the lead in the fifth as the Broncos scored three runs on three hits capped off by an RBI single by Mason Heyne to take a 4-1 advantage.
ETSU rallied to tie the game in the seventh as senior Derek Trent (Kingsport, Tenn.),
and Scruggs hit back-to-back home runs. Trent's two-run home run to left was his first of the season, while Scruggs hammered a 0-2 pitch over the left field fence to make the score 4-4.
In the bottom of the eighth, the Bucs grabbed their first lead of the game as junior right fielder John Long (Anderson, S.C.) drove in Pratt from second on a double down the left field line.
Scruggs led the way offensively for the Bucs going 3-for-4 in the contest with a triple, home run and scored two runs. Junior Bo Reeder (Cookeville, Tenn.) earned his second straight save throwing a scoreless ninth to close out the game.
For more information on ETSU baseball and all other Buccaneer athletic news please visit www.ETSUBucs.com.
ETSU 5, Rider 4 (Feb. 26, 2011 at Johnson City, Tenn.)
Rider............. 001 030 000 - 4 7 1 (0-2)
ETSU............ 001 000 31x - 5 9 2 (4-1)
Win - Ben Booker (2-0) Loss - Chris Taylor (0-1)
HR - ETSU - Derek Trent (1); Matthew Scruggs (2)


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Residents who would like to personally contribute to the quality of life in the City of Santa Clara are encouraged to apply to serve on any of the City's Board or Commissions. Residents may place their names on a list to be contacted whenever an opening occurs.
To be placed on the database maintained by the City Clerk's Office, call 408-615-2220 or email firstname.lastname@example.com.
Commissions include the Board of Library Trustees, Civil Service Commission, Cultural Commission, Historical & Landmarks Commission, Parks & Recreation Commission, Planning Commission, Senior Advisory Commission and Youth Commission. There is also a Housing Rehabilitation Loan Committee, ADA Advisory Committee, Bicycle Advisory Committee and other committee opportunities to serve.
The City of Santa Clara currently has vacancies on two Commissions: Civil Service Commission and the Parks & Recreation Commission
The deadlines to receive applications for each commission, interview dates and times are as follows:
Parks & Recreation Commission
Deadline to apply May 23, 2014, 5 p.m.
Interview dates & times June 10, 2014, 6 p.m.
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Deadline to apply May 23, 2014, 7 p.m.
Interview dates & times June 10, 2014, 7 p.m.
Interviews for Parks & Recreation Commission will held in the City hall Reception Lobby and the Civil Service Commission will occur in the Council Chambers during the regularly scheduled Council meeting.
If you are a registered or eligible voter (or in the case of the Youth Commission, a Santa Clara resident, age 12-19), and are interested in serving on any board/commissions/committees, or just want more information, contact the City Clerk's Office, 1500 Warburton Ave., Santa Clara, CA 95050. You may call the Clerk's Office on weekdays at 408-615-2220, email firstname.lastname@example.com or visit the online website at www.santaclaraca.gov.
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Thank you for visiting the City of Santa Clara website.


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If you can dream it, you can do it. -Walt Disney
Quality is a great business plan. -John Lasseter
Let's make some funny pictures. -Tex Avery
I never considered a difference of opinion in politics, in religion, in philosophy, as cause for withdrawing from a friend. -Howard Zinn
When critics sit in judgment it is hard to tell where justice leaves off and vengeance begins. -Chuck Jones
And what do you benefit if you gain the whole world but lose your own soul? -Jesus
A man should never neglect his family for business. -Walt Disney
What's most important in animation is the emotions and the ideas being portrayed. -Ralph Bakshi
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The New Forum Kicks Ass!!!
Share your views on the state of the Animation Industry.
7 posts • Page 1 of 1
The New Forum Kicks Ass!!!
I had to be the first guy to swear in a title of one of our new threads in the new forum! But it was appropriate, because the new forum kicks much ass!
Don't worry, I won't do it all the time...I just had to be the first.lol
What are your feelings on the this new message board?
Re: The New Forum Kicks Ass!!!
I agree. A great dimension to the site. From my end after working it on its first day in the open, I have to say that's it's been a lot of fun for me. Extremely easy to get visual content up, both video and graphics. I like tying the two forums together through the day and knowing that I can easily get video content related to a topic up on AN and keep it on AN as opposed to linking to another site.
Whatever we want to do in that regard we can do it. For me, it makes working the forums a more exciting and creatively stimulating experience as opposed to dealing with just text. I feel relaxed and more into it.
Rocks big time. Very happy about it.
Daily Z
Re: The New Forum Kicks Ass!!!
Word. When I come to the board, My thinking is totally different now. From the design to the functionality it really lends itself to a fun dynamic experience.
Re: The New Forum Kicks Ass!!!
It looks great guys. Congratulations on an awesome job! I'm dying to start playing with all the features and customize my avatar and signature. I'm finishing up a project right now and moving to Washington D.C., so I don't have a lot of time right now...but I can't wait.
...again, great job!
Re: The New Forum Kicks Ass!!!
I've been following AN off & on for the past few years but when I read that you launched a new website, I knew I had to join. So far, it looks good ... a bit sluggish though. All part of the growing pains
I look forward to these new forums and getting to know everyone a bit more.
Re: The New Forum Kicks Ass!!!
HEY GUYS! Welcome!
It runs fast for me!
Re: The New Forum Kicks Ass!!!
7 posts • Page 1 of 1


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BonBonBar's yummy Orange Bars combine pecan nougat bars with candied orange peel, salted caramel and...chocolate, of course! Photo by Corey Lugg | THE NIBBLE.
Product Reviews / Main Nibbles / Chocolate
America's Boutique Chocolatiers:
Why aren't more people producing great handmade candy bars? I can't answer that question, but Nina Wanat knows a good idea when it hits her, and her candy bars are always a good idea. Her packaging is not showy, and her confections are of relatively modest appearance, but don't let that deter you.
Try the Orange Bar, a two-layered beauty of nougat with pecans on the bottom and orange-peel-infused salted caramel atop. It's covered in dark chocolate and decorated with a strip of candied orange peel dusted with muscovado sugar. Everyone at THE NIBBLE offices swooned over it.
If you like more of an emphasis on nuts in your candy bars, the Caramel Nut Bar is for you. That's a sectioned, dark chocolate-covered bar filled with salted caramel, organic mixed nuts, and cacao nibs.
Nina even has a Scotch Bar that uses a single malt, and enough of it to make a Scotch lover take notice. Our editorial director, a connoisseur of single malts, gave it the thumbs up...and declared it an excellent gift for any Scotch drinker you know (she included herself on the list and asked for half a dozen, plus the Orange Bars). The use of salt is subtle; you'll taste it, but it doesn't overwhelm the other flavors.
As a non-bar bonus, BonBonBar offers marshmallows and hot chocolate mix. I haven't tried the latter, and her Vanilla Marshmallows are very nice, but the Passion Fruit Marshmallows were a revelation (I normally don't care for anything flavored with passion fruit, but I could eat these all day—and they disappeared in the office faster than you could say marshmallow). Sorry, Los Angelinos, there's no BonBonBar retail store as of this writing, but you can find her products at other local emporia...or buy them online like the rest of us.
Passion Fruit Marshmallows in the front, followed by Vanilla Marshmallows. Delicious for dipping in a Valentine's Day chocolate fondue.
Photo by Corey Lugg | THE NIBBLE.
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Telephone 1.323.843.4557
Freshly made Caramel Nut Bars.
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The Pre-Professional Department resides in the Leighty-Tabor Science Center, an 83,000-square-foot facility built in 2002. Its state-of-the-art labs serve all of the natural sciences at Millikin University, including Biology, Chemistry, Nursing and Pre-Professional Studies. The faculty of these departments work together to provide quality courses, lectures and labs for Pre-Professional majors.
The Pre-Professional Program at Millikin University dedicates its energy to preparing individuals for entry into and success in a post-graduate medical sciences program. Each student receives a liberal arts education with a firm foundation in science. Courses in science, social science, arts, and language balance those in biology and/or chemistry.
Individual monitoring of academic and professional goals and one-on-one contact with the program director set our program apart from others. Personalized mentoring invites you to become part of a community of learners and helps you focus on your coursework. Local internships and service opportunities provide hands-on enrichment and education, while an updated health-career library informs students of growing career opportunities.
Finally, and perhaps most importantly, Millikin offers on-campus admission exam preparation in the form of courses and review sessions. These sessions help you develop the skills needed to test into the post-graduate program of your choice.
For further details, see our program's Mission Statement.
This website was prepared with financial assistance from a grant by the US Department of Education. However, its contents do not necessarily represent the policy of the Department of Education, and you should not assume endorsement by the Federal Government.


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Plagne Centre. Chalet L'Alpicois is a well-located freestanding chalet with the piste and lifts conveniently close. A 300 metre walk (approx.) to the end of the road takes you to the Capella piste from where it is an easy ski to the lifts of Plagne Centre. The shops and restaurants of Plagne Centre are just a 530 metre walk (approx.) from the chalet. This unique chalet is set amongst a cluster of freestanding chalets in the Lotissement de la Foret area of Plagne Centre. Refurbished for 2014/15, Chalet L'Alpicois is now all en suite, with plenty of comfortable seating, an open fire in the living room and a large balcony, making this a welcoming chalet to return to at the end of a great day in La Plagne. Latest images of the chalet will be available to view on the website from this autumn.NB The beds in this chalet are slightly smaller than the French norm.
Ground floor:Room 1 = small twin plus extra single bed with shower and WCRoom 2 = twin with shower and WCRoom 3 = twin plus extra single bed with shower, WC and terrace Upper floor:Room 4 = large twin with shower and WC and double doors to balcony• open-plan living/dining room with fireplace and balcony• separate WC Chalet staff live out.
The chalet host will have a day off during the course of the week, during which you will be required to eat out.
Iglu acts as a travel agent for disclosed principals/tour operators, further details
of which will be made available at the time of booking. Your holiday contract will
be bound by the tour operators terms and conditions.
Prices are shown for a basic room or unit, can be subject to change and are indicative
until time of booking. Please call on 020 8544 7114 for prices for other travel
and room options for this property.
The price shown includes 7 nights accommodation,
with flights from Gatwick.
Prices shown are per person per week based on maximum occupancy. Descriptions and
quality ratings are provided by the Tour Operator (or one of the Tour Operators
where similar holidays are offered by more than one supplier). We reserve the right
to make minor adjustments to the description or rating based on staff and customer
feedback. For payments by credit card, a 2.5% credit card charge (4% for AMEX) will
be added to your invoice. If you pay by a UK issued debit card, there is no charge.
All bookings are subject to a £5 per person Passenger Admin Fee charged by Iglu.com.
Please note: the above prices are not inclusive of tourist tax which in this instance
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The end result of this simple project is a 100% plastic map that is thinner, lighter, and more durable than paper.
Sure, plastic paper can be purchased for printing water resistant maps however, it is expensive, and usually thicker and stiffer than regular paper; making it difficult to fold into a pocket sized, ready reference.
This process produces a map that is only slightly thicker than a garbage bag; that is waterproof, and best of all, can be folded (or crumpled) without damage for stuffing in a pocket.
Step 1: Size and Weight Matters
Nice abstract art but, useless for orienteering.
I like to print my own maps of an area when we go hiking. A trail map, and maybe a topographical (Topo) map, and a road map for directions to the trail head or pick-up point.
The approach used for years, to keep hiking maps from the elements involved storing them in a zip top plastic bag. Folded into quarters, it looks like the map on the left below. It keeps maps dry, however, the folded baggie only seems to fit in the square shaped pockets of cargo pants, and eventually, the folding and unfolding takes its toll on the bag and the paper inside.
Also, There is always a chance the maps will get wet every time the bag is opened. And many times, the bag has to be opened to shuffle through multiple pages for various sections of the trail.
For the most part, this method works, and I will probably continue to carry master maps in this fashion. But, sometimes, especially when traveling in familiar areas, all that is needed is a quick glance at a map to confirm the trail you want is to the left or right ahead.
This is where this Instructables map technique excels... A ready reference thin enough to be stuffed in a pocket, durable enough to survive multiple crumpling and best of all...weatherproof.
It is important to understand the limitations of this process.
Plastic film shrinks when heated so, the map scale will no longer be correct (i.e. a 1/4 inch will no longer equal a mile, etc.)
The fidelity of fine map details may be difficult to discern. Printer ink does not absorb into the plastic film so, the surface tension of the ink will will blur small text and fine details.
Despite these drawbacks, this process will produce a reusable map suitable to tuck in a shirt pocket for reference on the trail in the worst weather, or as a very compact emergency back-up map tucked away in a survival kit.
I often use Water Seal (Thompsons or Olympic) and just paint it on maps - either USGS or software-printed maps. The water seal soaks through the fibers and you end up with a "Write-on-the-Rain" material - can write on with pen or pencil, folds, and is more resillient than plain paper. A gallon is about $15 bucks (compared to $10 for 4oz of some commercial "Map Seal") and you can do a gazillion maps.on a gallon.
Tip- paint them on something you don't mind water sealing, since the sealant soaks through the paper. I do it on my picnic table and kill two birds with one stone.
However I have been printing Astronomy maps for years for nighttime usage by printing on plain copy paper(Cheap)!!!! and spraying with quick dry clear lacquer , then let dry for 10 min in the oven and it is permainately waterproof and fold-able.
may I ask why the drying in the oven? Must it be a a certain temp
If you use a warm oven (~250F) it dries quickly and the ink doesn't have time to bleed. Amateur astronomers and scouts have been using this trick for years. As clever as this 'ible is (and it is), the spray can laquer trick gives better results with a lot less effort.
I had an idea as far as the print detail goes. haven't tried it on your process but maybe it would work. I remember buying raw shrinky dink material and trying to do colored pencil drawings on them. The surface is pretty slick and didn't allow the color to stay on. So I lightly sanded the surface to be colored with fine sandpaper to give it a bit of texture. Maybe it could work her. But I don't know if that would work on the thin garbage bag to correct the ink issue.
Anywho....Great instructable! and Cheers!
The real advantage of this instructable is that it produces a map that can be stuffed in a pocket without the bulk. Folding will not create dammaging creases or delamination. The map is as thin and flexable as a plastic baggie.
Thanks :-)
Freezer paper is also a good substrate for running things through the printer. I think you would still want to pre-shrink the garbage bags, but if you happened to have some other non-shrinkable plastic you wanted to use, you could stick it to the freezer paper. Just iron it, same way - the shiny side is wax, so put something underneath it to avoid mussing your ironing board. It won't delaminate until you pull it off.
Using tissue paper (stuck to freezer paper) plus the baggie idea would yield a lightweight map without the hassle of smeary ink. Do baggies laminate to themselves? I'll have to try that... many applications if so. I know you can use them with a heat sealer, so maybe if you run it through the laminator on super-hot. (Or just iron it to melt it.)
There's also a substance called "bubble jet set" which will allow you to inkjet print onto fabric and it will be waterproof. I've used it myself and while I wouldn't necessarily trust it for a long-term T-shirt, it should work quite well for a map. Men's hankerchiefs would be lightweight and still have enough thread to produce enough detail.
Cool instructable!
http://www.bryerpatch.com/faq/bjs.htm - bubble jet set
Great Idea! Will try it out on NOAA's Booklet Charts for help Navigating
Coastal Waters.
I will certainly try this one!
What that accomplished was to prevent the toner from fusing to the paper. Make sure to choose a printer that spits the paper out face-up, because the toner will just be sitting on the paper. You can blow on it or brush a finger across it and the toner will move around like a sand painting.
What it's good for is you now have a loose layer of toner held onto paper by nothing more than static electricity. If you print the image reversed, and then lay the paper on a piece of wood, the toner will transfer to the wood. The guy I saw make the best use of this technique would then carve a person's likeness, or their dog, or a favorite design into a desk sign or plaque, or whatever you wanted. He couldn't draw a lick, but he could sure trace the lines of the printed image!
This is also good for making the name 'chops' like the Chinese use to sign documents. (I worked in Taiwan for a while.) When you're carving the little stone stamp, you don't want to get done to find out that a character is backwards, so some of the shops that make them have the hacked printers. They print the design right-ways, and pick up the toner on the bottom of the stone. Then when they are done carving, they know the design will be right.
How it could be used here is that you could transfer the toner to the white plastic, add the clear and then make one sandwich to iron. All the shrinkage would occur to both pieces of plastic at the same time, so distortion should be minimized, and it keeps you from running plastic through your printer.
I think for inkjets, the ible is the best possible way. I thoroughly agree that using pre-shrunk plastic is good. I am mainly bringing this up to tell people about the toner transfer capability of a hacked laser.
Also, how well does your map react to being repeatedly folded and refolded?
Fantastic ible!


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Ferro earnings top estimates despite sales decline
4:03 PM MDT | April 24, 2013 | Vincent Valk
Ferro has reported first-quarter net income of $800,000 down 78.9% from the same quarter last year, on sales down 9.1% year-on-year (YOY), to $418 million. Excluding discontinued operations, adjusted earnings from continuing operations totaled 10 cts/share, beating analysts' consensus of 5 cts/share, as reported by Thomson Reuters (New York). Ferro announced the sale of its loss-making solar pastes business during the quarter, and announced the sale of its pharmaceuticals business on 1 April. Lower volumes, selling prices and a less-favorable product mix...
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We carry jogging strollers, oftentimes considered as a backup for a standard stroller or sometimes as an everyday stroller (especially for city dwellers). Featuring large, sturdy tires, sleek frames, handgrip brakes and all-terrain suspension systems, jogging strollers perform well on almost any surface.
Travel systems are a great convenience for busy new parents. Beginning with a standard stroller, they include an infant car seat that snaps onto the stroller, allowing parents to move the infant to and from easily. Most travel systems come with a car seat base that stays in the car so that you can simply snap your baby seat into the base rather than buckling and unbuckling the seat into the car. Once the infant seat is outgrown, travel systems perform the same as standard strollers.
Our standard strollers often feature roomy seats, large wheels, storage space and extras like built-in music systems and ergonomic handles. They perform well and are versatile. Our lightweight strollers have many of the same features as larger strollers, such as reclining seats, storage baskets and sunshades, great for travelers and perfect for trips to the mall or zoo.
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Wednesday's Morning Zone Roster
Today is a busy day around the Morning Zone, kicking off in the 6:37AM MDT segment with our Watchdog on Wall Street calling in from the Republican National Convention.
7:07AM MDT: It's early open line time for those who need to head off to work and can't call in later in the program. Pick up the phone and


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Life's tough and deadly for fish in urban aquatic jungle
Even in the finest of fisheries, with excellent water quality, abundant natural cover and easily-available forage, a fish's life is fraught with danger. Something's always out to eat them. Or worse. In less-than-wonderful waters, things are even tougher. There, fish face challenges their cousins in healthier waters seldom, if ever encounter. It's like the difference […] [Read More]


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Nominations for the annual Holiday Outdoor Decorations Contest will be accepted from Nov. 25 through Dec. 6. Decorate the exterior of your home or business in cheerful, colorful holiday décor and talk to your neighbors about doing the same. Nominate yourself, your neighbor, or one of the brightly lit homes or businesses you've seen around the City.
Nominations for best outdoor decorations in five categories (residential, business, neighborhood, best tree, and special recognition) will be evaluated by a team of judges Tuesday, December10. Winners will be announced December 11. A list of winners along with the full list of entrants in convenient driving order will be available on the City's website. Winners will be acknowledged at a City Council meeting in early January 2014.
Thank you for your Holiday Outdoor Decoration Contest Nomination!
For more information, contact the City Manager's Office at 1-408-615-2210.
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All parents eventually face the decision to leave their child home alone for the first time. Whether they are just running to the store for a few minutes, working during after-school hours or summer vacation, parents need to be sure their children have the skills and maturity to handle the situation safely. Being trusted to stay home alone can be a positive experience for a child who is mature and well prepared. It can boost the child's confidence and promote independence and responsibility.
However, children face real risks when left unsupervised. Those risks, as well as a child's comfort level and ability to deal with challenges, must be considered. The U.S. Department of Health and Human Services created a factsheet that provides some tips to help parents and caregivers when making this important decision.
Even after you decide your child is ready to stay home alone, it's natural to feel a little anxious when the time comes. These helpful steps can make the transition easier for you both:
Childproof Your Home
Reduce the risk of health and safety hazards by locking up or removing the following items:
- alcohol
- prescription medication and over the counter medicine
- tobacco
- car keys
- lighters and matches
- guns (if you do own one, make sure it's locked up, unloaded, and stored away from ammunition)
Set Ground Rules
Consider setting rules about:
- having friends over
- TV and computer rules (set parental controls)
- kitchen and cooking guidelines (do you want them using the oven?)
- not opening the door for strangers
- answering the phone
- not telling anyone he or she is home alone
Touch base
Set up a schedule for calling. Make sure your child knows when you're available and when you might be unable to answer a call. You might have your child call in as soon as they get home, especially if they are coming home to an empty house, or set up a time when you'll call home to check-in. The goal is to figure out something that is convenient for the both of you.
Have on Hand
Make sure your kitchen is stocked with healthy snacks and emergency supplies. Post important phone numbers and leave flashlights in an accessible place in case of a power outage. It's also a good idea to set out the precise dose of medication your child needs to take, but be mindful of leaving the bottle out if younger siblings are present as they may ingest.
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Also Dr. to explain the treatments.
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Victoria Family Eye Care
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Young, Dr. Timothy N.
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