text:
Every year, the government funnels tens of billions of dollars toward scientific research — research that you help pay for with your taxes. These government-funded investigations give rise to an average of 65,000 peer-reviewed papers a year. Many of these papers, however, remain inaccessible to you unless you happen to subscribe to the journal in which they're published, and some of these journals are pretty expensive.
Think you should be able to access this research free of charge? So do a lot of other people — in fact, legislation was just introduced that could make open-access government-funded science a reality: Wired's David Dobbs gives a good summary of recent events leading up to its introduction:
The open-science revolt, catalyzed just a few weeks ago as a reaction to publisher Elsevier's backing of a clumsy bill introduced to the U.S. .Congress, now has a champion in that Congress, Representative Mike Doyle, a Democrat of Pennsylvania, who has introduced legislation to encourage open access to government-sponsored science.
The legislation in question is a bill entitled the Federal Research Public Access Act or FRPAA. The upshot of the bill is that it would make papers that report on government-funded research publicly available within six months of their publication, regardless of where they were printed (be it Nature, PNAS, etc.), or the research institution that submitted them to be published.
You can read the Federal Research Public Access Act here, and learn more on the FRPAA website, but if you're looking for a good introduction to the idea of open access science in general, I highly recommend checking out this feature, also penned by Dobbs.
The article was actually written back in May of last year, but it explores the concept of free science in greater detail, and provides some context for why the idea — which, for years, has failed to pick up steam outside of small, dissociated segments of the scientific community — finally seems to be gaining some momentum. The piece begins:
On Father's Day three years ago, biologist Jonathan Eisen decided he'd like to republish all his father's papers. His father, Howard Eisen, a biologist and a researcher at the National Institutes of Health, had published 40-some-odd papers by the time that he died by suicide at age 45. That had been in Febuary 1987, while Jonathan, a sophomore at college, was on the verge of discovering his own love of biology. At the time, virtually all scientific papers were just on paper. Now, of course, everything happens online, and Jonathan, who in addition to researching and teaching also serves as an editor for the open-access, online-only journal PLoS Biology, knows this well. So three years ago, Jonathan decided to reclaim his father's papers from print limbo and make them freely available online. He wanted to make them part of the scientific record. He also wanted, he says, "to leave a more positive presence" — to ensure his father had a public legacy first and foremost as a scientist.
How hard could it be? Howard Eisen had been a federal employee, so his work rightly lay in some sense in the public domain. And Jonathan, as an heir, presumably owned copyright anyway, along with his brother Michael (also a biologist, and one of the founders of the Public Library of Science, the innovative journal group that publishes PLoS Biology). Yet to the brothers' continuing chagrin, Jonathan has found securing and publishing his father's papers to be far harder than he expected.
For instance, even though Jonathan has access to the enormous University of California library system, which subscribes to a particularly high number of journals, he often can't even find some his father's papers. And when he finds a paper in a journal the university doesn't subscribe to, he is asked to pay as much as $50 to read the paper - even though his father did the work with public funds. He's not alone; one recent study found that even most university researchers have access to only about half the papers they need to cite for a given bit of research. Just yesterday, in fact, Jonathan asked on Twitter if anyone could send him a copy of one of his father's paper and confronted a paywall asking for his credit card number. "I ain't payin'," he replied.
Meanwhile, Jonathan has found and downloaded the PDFs for about half his father's papers, but he remains uncertain whether he could safely post them on his website. While some publishers allow such "collegial sharing," others leave their policies unclear, and he worries about getting sued. His brother urged Jonathan to post them.
"Come on," Michael wrote in a comment at Jonathan's blog. "I DARE them to sue us."
Jonathan has posted the whole list at his blog and uploaded what PDFs he could obtain, and so far he has not been sued or asked to take them down. Yet he remains wary and unsatisfied. He knows that few researchers will find his father's papers if they reside only on his web page. So for now, his father's work remains buried in an old structure - a calcified matrix. Though Jonathan bangs away at the surrounding rock, he knows he hasn't really pried the work loose. This frustrates him on two fronts: It stops him from freeing his father's work. And it confirms to him science, which should be a fluid medium, has much of its content still trapped in old structures.
"I started this partly to test how hard it would be to try to make science more available in the current system," he says. "I'm finding that even with my father's papers, or even with my own, it's not very easy."
Jonathan Eisen's quest has solidified his conviction that science needs to radically rework the way it collects and shares its data, methods, and findings. He has plenty of company. A growing number of prominent scientists want to replace the aging journal system with something faster, cheaper, and richer. The current system, they note, grew out of meeting notes and journals published by societies in Europe over three centuries ago. Back then, quarterly or monthly volumes could accommodate the flow of ideas and data from most disciplines, and the printed journal, though it required a top-heavy, expensive printing and publishing infrastructure, was the most efficient way to share those ideas.
"But now," says Jonathan Eisen, "there's this thing called the Internet. It changes not just how things can be done but how they should be done."
As Stanford biochemist and PLoS co-founder Patrick Brown put it a few years ago, "What seemed an impossible ideal in 1836, when Antonio Panizzi, librarian of the British Museum, wrote, 'I want a poor student to have the same means of indulging his learned curiosity, … of consulting the same authorities, … as the richest man in the kingdoms,' is today within reach. With the Internet, we have the means to make humanity's treasury of knowledge freely available to scientists, teachers, students and the public around the world."
"The existing system worked well for quite a while," says Jonathan Eisen. "But it was not designed by theory. It was designed by constraints." In a world that provides communications conduits far larger and faster, those constraints have now made science's traditional pipeline a bottleneck.
Continue reading on Wired, and be sure to check out Dobbs' ongoing coverage on free science and the FRPAA


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text: Alan stumbles upon an opportunity to make money. Meanwhile, Walden has to address his codependency issues, on TWO AND A HALF MEN, Thursday, April 24 (9:01-9:31 PM, ET/PT) on the CBS Television Network. Pictured L-R: Amy Pietz as Angie and Jon Cryer as Alan Harper Photo: Michael Yarish/Warner Bros. Entertainment Inc. ©2014 WBEI. All rights reserved.


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|Volleyball » News » Schedule » Roster » Coaches » Statistics
Volleyball Looks Toward the 2001 Season
Tuesday, August 14, 2001The 2001 East Tennessee State volleyball team will be led by Deane Webb, the sixth head coach in the 27 years of the program. Webb comes to Johnson City from Indiana Wesleyan University in Marion, Ind. While at Indiana Wesleyan, Webb led his teams to a 134-51 overall record and was a two-time conference coach of the year honoree. Webb took over a team that finished 7-31 in 1996 and by the time he was done, set school records for wins (43) and winning percentage (43-4/.915) during the 2000 season. Webb now takes over the helm of a Buccaneer volleyball team that lost three seniors from a team that finished 16-13, 12-8 in the 2000 Southern Conference standings (sixth). Those seniors, Jena Policastro, Courtney Sussillo and Kellie Wallace missed just one combined match last season (83 of a possible 84). Losing three seniors may take a toll on some teams; the Bucs will have no shortage of leadership in the 2001 campaign. Leading ETSU this season will be seniors Carey Cavanaugh, Amber Ciskowski and Anastasia Dishong. Cavanaugh has been the quarterback of the ETSU offense since her transfer from Ohio State in 1999. In 2000, she was an All-Southern Conference performer for the second consecutive season and became just the fourth player in ETSU history to record 2,000 career assists. Ciskowski led the Bucs with 311 kills (2.85 pg) during the 2000 season. She registered double-digit kills in 17 of 29 matches last year. Together, Cavanaugh and Ciskowski make up one of the most feared combinations in the Southern Conference. Dishong was sidelined during the 2000 season due to an injury. Aside from the three seniors, the 2001 Buccaneer squad will be stocked with young talent. The rest of the ETSU roster will consist of five sophomores and three freshmen. But these are not your typical sophomores. Many played key roles as freshmen in ETSU's success in the 2000 season. Webb will count on his established players as well as his young talent to make a run at the 2001 Southern Conference Championship. ON THE OUTSIDE The outside for the Buccaneers will be called upon to provide firepower and stability during the 2001 season. The ETSU outside hitters are one of the most experienced groups on the squad. The Buccaneers will field one of the top groups of outside hitters in the Southern Conference, Ciskowski (Phoenix, Ariz.), Rachel Shifflet (Axton, Va.) and Kate Steidle (Waynesboro, Va.). Ciskowski has been one of the most consistent performers on the team ever since her arrival in Johnson City. She has been an offensive and defensive leader during her ETSU career, averaging over 300 kills and 300 digs in her last two seasons. "Amber is a very hard worker," said Coach Webb. " We can count on her experience, stability and discipline to lead us on the court." Shifflet stepped in during the 2000 season and established herself as one of the best young players in the Southern Conference. She led the Buccaneers in kills per game (2.88) and had a career-high with 20 kills in a loss to Virginia Tech on Nov. 1. "We can count on Rachel to bring power to our offense," said Coach Webb. "She hits a heavy ball and will be counted on heavily in side-out situations." Steidle played a important role for the ETSU last season, putting together a streak of four consecutive matches where she registered double-digit kills. The Bucs were 3-1 in that stretch. All told, Kate had six matches with double-digit kills. "Kate is a fast, athletic, dynamic player," said Coach Webb. "With more experience, she can develop into a excellent player." Fellow sophomore Gretchen Rupert (Staunton, Va.) also came in as freshmen last season and contributed to the Bucs' success. Rupert saw action in four matches last season, notching a career-high with five kills and seven digs on Nov. 3 against The Citadel. "Gretchen will be playing on the left side this season," said Coach Webb. "She will add a lot of extra punch to the left side of the attack." Freshman Sara Scandiffio (Purcellville, Va.) will also play on the outside for ETSU this season. "Sara should provide a very good block to our right side," said Coach Webb. "As her offense develops, she could become a force for us on the right side." Scandiffio will also see time in the middle during the 2001 season. IN THE MIDDLE In the middle, the Buccaneers will be a young but talented group coming into the 2001 campaign. The only player on the Buccaneer roster that has seen significant time as a middle hitter at ETSU is sophomore Lissa Allen (Palm Bay, Fla.). Allen gained valuable experience last season, playing in 26 of 28 matches last season. Allen registered double-digit kills in four matches in 2000, three of which went on to become Buccaneer victories. "Lissa is a good all-around player that is able to hit a variety of sets," said Coach Webb. "She is already a good player. With more time and conditioning, she could become an excellent player." Allen will see time at front and back row positions during the 2001 season. Joining Allen in the middle will be freshman Natalie Atzinger (Louisville, Ky.). Atzinger played for the KIVA Volleyball Club, a high-level volleyball team in Louisville, Ky. She is a hard-working and disciplined player. "Natalie makes good decisions on the court," said Webb. "We will count on her to aid in running the offense." THE SETTERS The setters on the 2001 Buccaneer squad will be the strength of the team. The Bucs have one of the best setters in the Southern Conference in the two-time All-SoCon honoree Cavanaugh (Munroe Falls, Ohio). The Bucs may also have another star setter waiting in the wings with sophomore Jessica Miller (Knoxville, Tenn.) Cavanaugh, a very good athlete, is the confident floor general of the Buccaneers. Cavanaugh's inspired offensive and defensive play always seems to give the Bucs a boost in pressure situations, whether ETSU is trying to close out a match or come back from a deficit. "We will count on Carey's leadership and ability," said Webb. "Her individual leadership and intensity will contribute greatly to the team's success." The Buccaneers came to a crossroads in the 2000 season when Cavanaugh missed four matches in a crucial portion of the season. Losing their floor leader might be a problem for most teams, but ETSU turned to the then-freshman Miller, who did an admirable job. She stepped in as starting setter, leading the Bucs to a 2-2 record in four matches. In Cavanaugh's absence, Miller led the team with 7.31 assists per game and .46 aces per game. For the season, Miller finished fourth in the SoCon with .49 service aces per game. Miller will see most of her time this season as a defensive specialist, but can play setter if necessary. "Jessica is a steady back-row presence," said Webb. "She is a hard worker that can step in and set as needed." Possibly one of the most versatile players on the Buccaneer roster is senior Anastasia Dishong (Prescott, Ariz.). Dishong was moved from setter to defensive specialist prior to her junior campaign, but suffered an injury and missed the entire 2000 season. "Her back-row leadership will show through her inspired play and defense," said Webb. Another exciting Buccaneer newcomer is freshman defensive specialist Karen Freeburg (Newcastle, Ind.). Freeburg is a very good hitter and receiver that played outside hitter in high school, but will see action at both left side hitter and defensive specialist at ETSU. Freeburg played for the same Munciana Volleyball Club that Webb coached in Indiana. "Karen has a good jump serve and great ball control," said Webb. "She will bring stability to the team and will contribute right away." The Bucs will open up the 2001 season on Friday, Aug. 31 at the Winthrop University tournament in Rock Hill, S.C. Participating teams will be Winthrop, Georgia State and Georgia Southern. ETSU will open up its Southern Conference schedule on Sept. 4 with a trip to Western Carolina. The Bucs gained valuable experience during the 2000 season as many freshmen got the opportunity to step up and contribute. That playing time should translate into good team chemistry for the 2001 campaign. "Team chemistry is a major part of success," said Webb.
Volleyball Archives
2001-02 Volleyball


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GAB agrees to reject outside petition reviews
Kevin Kennedy. | Photo: Milwaukee Journal Sentinel
MADISON - Wisconsin's elections board has agreed not to accept independent reviews of recall petitions done by anyone other than Gov. Scott Walker and other targeted office holders and those who circulated the petitions.
The Government Accountability Board on Tuesday agreed to reject an offer made by a pair of tea party groups offering to turn over their own review of the estimated 1 million signatures submitted on the Walker recall petitions.
Board director Kevin Kennedy says there is no process in place for accepting information from outside groups and individuals.
"The legislature made it very clear about how government is supposed to act. Like in a court, you don't just allow people to walk in and say to the court, 'I've got additional evidence.' You want to have certain procedures in place for that," Kennedy told TODAY'S TMJ4's Lacey Crisp.
The four targeted state senators have until Thursday to challenge signatures. Walker's challenges are due Feb. 27 and Lt. Gov. Rebecca Kleefisch has until March 5.
The GAB has until March 19 to verify all of the petitions.
- Latest


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Steven Dubin
Professional Background
Educational Background
Ph.D., Sociology, 1982, University of Chicago
Postdoctoral Studies: University of Chicago and Yale University
Scholarly Interests
Selected Publications
biographical information
Steven Dubin comes to Teachers College after being a faculty member at Purchase College--State University of New York for 19 years. There he directed the Media, Society, and the Arts Program, which links the arts conservatories and the liberal arts, since 1988. He received his Ph.D. at the University of Chicago; in addition, he did postdoctoral work at both the University of Chicago and Yale University. Professor Dubin has also offered courses in the Columbia Summer Session since 1985.
He is the author of Bureaucratizing the Muse: Public Funds and the Cultural Worker (1987); Arresting Images: Impolitic Art and Uncivil Actions (1992, paperback edition, 1994 ; cited as a Notable Book of the Year by The New York Times , and by the Gustavus Myers Center for the Study of Human Rights); Displays of Power: Memory and Amnesia in the American Museum (1999; paperback edition, 2000), and the forthcoming Transforming Museums: Mounting Queen Victoria in a Democratic South Africa (2006).
Professor Dubin has won many awards, including the Fulbright-Hays Faculty Abroad Research Fellowship to South Africa, the SUNY Chancellor's Award for Excellence in Scholarship and Creative Activities, The Lady Davis Fellowship Trust Visiting Professorship at Hebrew University in Jerusalem, and residencies at The Bellagio Study & Conference Center (Bellagio, Italy), The Ragdale Foundation (Lake Forest, Illinois), and The Ucross Foundation (Clearmont, Wyoming).
He has written and lectured widely on public funding of the arts, censorship, transgressive and controversial art, obscenity, museums, and popular culture. His articles and reviews have appeared in Contemporary Sociology, American Journal of Sociology, Urban Life, Social Problems, Social Forces, Sociological Inquiry, Symbolic Interaction, Visual Anthropology, Journal of Aesthetic Education, Journal of Arts Management and Law, Curator Magazine, Nation, Jewish Currents, Common Quest, New Art Examiner and Art in America .
He is frequently sought for commentary by journalists, and Arresting Images was referenced in a 1992 court decision involving the police seizure of a painting in Chicago. In addition, Professor Dubin has become a free speech activist, breaking the story of corporate censorship by Mattel, Inc. in regards to "Art, Design, and Barbie: The Evolution of a Cultural Icon," a 1995 museum exhibition which he helped curate. His article "How I Got Screwed by Barbie" generated news coverage nationwide. He also dissected the evolution of the controversy over the 1999 exhibition "Sensation" at the Brooklyn Museum of Art, a story featured in Art in America .He has been traveling throughout Southern Africa for the past five years, including the countries of Namibia, South Africa, Mozambique, Lesotho and Swaziland. Some of the research he has done is reflected in his forthcoming book on the transformation of South African museums during the post-apartheid period, since 1994.
principal publications
Books: Transforming Museums: Mounting Queen
Displays of Power: Memory and Amnesia in the
Arresting Images: Impolitic Art and Uncivil Actions.
Bureaucratizing the Muse: Public Funds and the Cultural Worker.
Articles: "Our Man in Havana: Robert Mapplethorpe--Myths, Legends, Realities," Art in America, May, 2006.
"Uncivil Wars in Civil (-zed) Places," in MacDonald,
"Bring in da' Noise, Bring in da' Funk," pp. 18-25 in Ishumi/10. [A Decade of Democracy]
A&HG 4173: Arts in context
Permission of professor required. A seminar and lecture-style course designed to provide a multi-disciplinary and cross-cultural context for discussions of aesthetic, ethical, and political questions that define and challenge the responsible arts administrator's role.
A&HG 4575: Master's seminar in arts administration
Permission from program coordinator required. Full-time degree candidates only. Required for all master's students. Guided independent work culminating in the development of a comprehensive research plan and methodology to produce the culminating master's essay.
A&HG 4970: Supervised individual research in arts administration and arts education
Permission from program coordinator required. Independent research in arts administration.


text:
June 02, 2014
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A traditional family style Italian eatery offering authentic pizzas, pastas, salads and a great gelato. Enjoy the atmosphere of watching the chefs at work while dining in or select from the take away cabinet if you are on your way to your gate.
Trattoria Prego also serves great coffee and a fine selection of Italian and local wines and beers.


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Wendy Spencer, CEO Of the Corporation for National and Community Service, shares some statistics about volunteering in the United States, highlights of this year's "Volunteering and Civic Life in America."
After 25 seasons, there are still some firsts on The Amazing Race.
The new lineup of co-hosts on�The View�are having a hard time jelling with each other.On...
By Frank MulliganMore Content NowThere are subtle indications that your perspective on Christmas may be Grinch-like. For instance, do you steadfastly maintain that elves are illegal immigrants?
Natalie Anderson�says one of the hardest things about participating in Season 29 of
Monday the Rocky Ford Police advised Tri-County Family Care Center of possible stolen merchandise from the TCFCC office.
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Thunderstorms in the area this morning through this evening
Near wild flowers that bloom in early autumn cast a magical glow while presaging the coming of winter.
Once known as "Love Apples", tomatoes have outlived their checkered reputation to achieve a place of respect and reverence in the cornucopia of late summer's bounty.
August is the best month to divide perennials because the ground is warm and the sun high, so with water the transplants can develop a good root system before winter. Most will flower next year.
Revel in the blooms and flora at these 14 stunning botanical gardens around the world.
There is this myth about perennials returning each year and producing a sequence of bloom that continues all season long. Well, it's just not so.
There have been some studies about mowing efficiency . The fewer turns one makes and the longer the straight stretches are, the less time it takes.
Brown and bare spots of lawn can be brought back to life easily.
A rain barrel is a water-efficient way of supplying your garden with a renewable source of water.
Sep 20, 2011; 5:00 AM ET Why do leaves change from green to beautiful shades of red, orange and yellow in the fall?


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Late-stage fluorescence-based dose-response cell-based assay to identify agonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4): Sphingosine 1-Phosphate Receptor 1 (S1P1) counterscreen assay
Name: Late-stage fluorescence-based dose-response cell-based assay to identify agonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4): Sphingosine 1-Phosphate Receptor 1 (S1P1) counterscreen assay. ..more
BioActive Compound: 1
Related Experiments
Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center
Center Affiliation: The Scripps Research Institute (TSRI)
Assay Provider: Michael Oldstone, TSRI
Network: Molecular Library Screening Center Network (MLSCN)
Grant Proposal Number: U01 AI074564
Grant Proposal PI: Michael Oldstone, TSRI
External Assay ID: S1P1_AG_BLA_384_3XEC50
Name: Late-stage fluorescence-based dose-response cell-based assay to identify agonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4): Sphingosine 1-Phosphate Receptor 1 (S1P1) counterscreen assay.
Pandemic influenza represents a significant public health threat, due in part to immune cell-mediated lung tissue damage induced during viral infection. Sphingosine 1-phosphate (S1P) is a bioactive phospholipid released by activated blood platelets and serves to influence endothelial integrity, lung epithelial integrity (1), and lymphocyte recirculation (2-5) through five related high affinity G-protein coupled receptors. Recently, modulation of S1P receptors locally in the lungs was shown to alter dendritic cell activation and accumulation in the mediastinal lymph nodes, resulting in blunted T cell responses and control of immunopathological features of influenza virus infection (6). Reports showing that S1P5 expression is very low in dendritic cells but that S1P4 is highly expressed (7), suggest that chemical activation of the S1P4 receptor subtype in the airways could be efficient at controlling the immunopathological response to viral infection. S1P4 is coupled to Galphai and Galphao G-proteins and activates ERK MAPK and PLC downstream pathways (8). Thus, the identification of compounds that act as selective S1P4 agonists will provide insight into S1P4 biology and may serve as useful tools to limit lung tissue injury resulting from influenza infection.
1. Sanna, M.G., J. Liao, E. Jo, C. Alfonso, M.Y. Ahn, M.S. Peterson, B. Webb, S. Lefebvre, J. Chun, N. Gray, and H. Rosen, Sphingosine 1-phosphate (S1P) receptor subtypes S1P1 and S1P3, respectively, regulate lymphocyte recirculation and heart rate. J Biol Chem, 2004. 279(14): p. 13839-48.
2. Forrest, M., S.Y. Sun, R. Hajdu, J. Bergstrom, D. Card, G. Doherty, J. Hale, C. Keohane, C. Meyers, J. Milligan, S. Mills, N. Nomura, H. Rosen, M. Rosenbach, G.J. Shei, Singer, II, M. Tian, S. West, V. White, J. Xie, R.L. Proia, and S. Mandala, Immune cell regulation and cardiovascular effects of sphingosine 1-phosphate receptor agonists in rodents are mediated via distinct receptor subtypes. J Pharmacol Exp Ther, 2004. 309(2): p. 758-68.
3. Gon, Y., M.R. Wood, W.B. Kiosses, E. Jo, M.G. Sanna, J. Chun, and H. Rosen, S1P3 receptor-induced reorganization of epithelial tight junctions compromises lung barrier integrity and is potentiated by TNF. Proc Natl Acad Sci U S A, 2005. 102(26): p. 9270-5.
4. Wei, S.H., H. Rosen, M.P. Matheu, M.G. Sanna, S.K. Wang, E. Jo, C.H. Wong, I. Parker, and M.D. Cahalan, Sphingosine 1-phosphate type 1 receptor agonism inhibits transendothelial migration of medullary T cells to lymphatic sinuses. Nat Immunol, 2005. 6(12): p. 1228-35.
5. Alfonso, C., M.G. McHeyzer-Williams, and H. Rosen, CD69 down-modulation and inhibition of thymic egress by short- and long-term selective chemical agonism of sphingosine 1-phosphate receptors. Eur J Immunol, 2006. 36(1): p. 149-59.
6. Jo, E., M.G. Sanna, P.J. Gonzalez-Cabrera, S. Thangada, G. Tigyi, D.A. Osborne, T. Hla, A.L. Parrill, and H. Rosen, S1P1-selective in vivo-active agonists from high-throughput screening: off-the-shelf chemical probes of receptor interactions, signaling, and fate. Chem Biol, 2005. 12(6): p. 703-15.
7. Maeda, Y., Matsuyuki, H., Shimano, K., Kataoka, H., Sugahara, K., and Chiba, K., Migration of CD4 T cells and dendritic cells toward sphingosine 1-phosphate (S1P) is mediated by different receptor subtypes: S1P regulates the functions of murine mature dendritic cells via S1P receptor type 3. J Immunol, 2007. 178(6): p. 3437-46.
8. Toman, R.E. and S. Spiegel, Lysophospholipid receptors in the nervous system. Neurochem Res, 2002. 27(7-8): p. 619-27.
Sphingosine Receptor, Sphingosine-1-phosphate receptor 4, S1P4, Sphingosine-1-phosphate receptor 1, S1P1, EDG6, EDG1, LPC1, agonist, activator, GPCR, 384, Tango, FRET, GAL4-VP16, beta-arrestin, beta-lactamase, BLA, reporter gene, fluorescence, primary, late stage, late stage AID, powders, Scripps, Scripps Research Institute Molecular Screening Center, SRIMSC, Molecular Library Screening Center Network, MLSCN
Assay Overview:
The purpose of this assay is to determine whether powder samples of compounds identified as active in the assay "Late-stage fluorescence dose-response cell-based assay to identify agonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4): Synthesized compounds" (AID 463107) were nonselective agonists as assayed by activation of S1P1. This assay uses Tango S1P1-bla U2OS cells which express the human Endothelial Differentiation Gene 1 (EDG1; S1P1) linked to a GAL4-VP16 transcription factor via a TEV protease site. The cells also express a beta-arrestin/TEV protease fusion protein and a beta-lactamase (BLA) reporter gene under the control of a UAS response element. Stimulation of the S1P1 receptor by agonist causes migration of the fusion protein to the GPCR, and through proteolysis liberates GAL4-VP16 from the receptor. The liberated VP16-GAL4 migrates to the nucleus, where it induces transcription of the BLA gene. BLA expression is monitored by measuring fluorescence resonance energy transfer (FRET) of a cleavable, fluorogenic, cell-permeable BLA substrate. As designed, test compounds that act as S1P1 agonists will activate S1P1 and increase well FRET. Compounds were tested in triplicate using a 10-point, 1:3 dilution series starting at a nominal concentration of 50 uM.
Protocol Summary:
U2OS cells were cultured in T-175 sq cm flasks at 37 C and 95% relative humidity (RH). The growth media consisted of McCoy's 5A Medium supplemented with 10% v/v dialyzed fetal bovine serum, 0.1 mM NEAA, 25 mM HEPES (pH 7.3), 1 mM sodium pyruvate, 100 U/mL penicillin-streptomycin-neomycin, 200 ug/mL Zeocin, 50 ug/mL Hygromycin, and 100 ug/mL Geneticin.
Prior to the start of the assay, cells were suspended at a concentration of 275,000/mL in Assay Medium (Freestyle Expression Medium without supplements). The assay was started by dispensing 10 uL of cell suspension to each well of a 384 well plate (10,000 cells/well), followed by overnight incubation at 37 C in 5% CO2 and 95% RH. The next day, 50 nL of test compound in DMSO (0.5 % final DMSO concentration), DMSO alone, or S1P (40 nM final nominal EC80 concentration) prepared in 2% BSA was added to the appropriate wells. Plates were then incubated at 37 C in 5% CO2 for 4 hours. After the incubation, 2.2 uL/well of the LiveBLAzer FRET substrate mixture, prepared according to the manufacturer's protocol and containing 10 mM Probenicid, was added to all wells. After 2 hours of incubation at room temperature in the dark, plates were read on the EnVision plate reader (PerkinElmer Lifesciences, Turku, Finland) at an excitation wavelength of 405 nm and emission wavelengths of 460 nm and 535 nm.
Selected datapoints were not included in the calculations because they were outliers. Prior to normalization, data were corrected by subtracting "background" for both emission channels (ie, fluorescence values from cell-free wells containing media and substrate only). To normalize assay data, these corrected values were used to calculate a ratio for each well, according to the following mathematical expression:
Ratio = I460 nm / I535 nm
I represents the measured fluorescence emission intensity at the enumerated wavelength.
The percent activation for each compound was calculated using well fluorescence as follows:
% Activation = 100 * ( 1 - ( ( Test_Compound - Median_High_Control ) / ( Median_Low_Control - Median_High_Control ) ) )
Test_Compound is defined as wells containing test compound and S1P
Low_Control is defined as wells containing DMSO
High_Control is defined as wells containing 5 uM S1P
For each test compound, percent activation was plotted against compound concentration. The reported EC50 values were generated from fitted curves by solving for the X-intercept value at the 50% activation level of the Y-intercept value. In cases where the highest concentration tested (i.e. 50 uM) did not result in greater than 50% activation, the EC50 was determined manually as greater than 50 uM.
PubChem Activity Outcome and Score:
Compounds with an EC50 greater than 10 uM were considered inactive. Compounds with an EC50 equal to or less than 10 uM were considered active.
Any compound with a percent activity value <50% at all test concentrations was assigned an activity score of zero. Any compound with a percent activity value > 50% at any test concentration was assigned an activity score greater than zero.
The PubChem Activity Score range for active compounds is 100-100, and for inactive compounds 0-0.
List of Reagents:
Tango EDG1-bla U2OS cells (Invitrogen, part K1520)
GeneBLAzer FRET B/G Loading Kit (CCF4-AM) (Invitrogen, part K1025)
Probenecid (Sigma, part P8761)
Freestyle Expression Medium (Assay media; Invitrogen, part 12338-018)
McCoy's 5A Medium (modified) (1X) (Invitrogen, 16600-082)
Fetal Bovine Serum, dialyzed (Invitrogen, part 26400-036)
NEAA (Invitrogen, part 1114-050)
Penicillin-Streptomycin-Neomycin antibiotic mix (Invitrogen, part 15140-122)
Sodium Pyruvate (Invitrogen, part 11360-070)
PBS without calcium or magnesium (Invitrogen, part 14190-136)
HEPES (Invitrogen, part 15630-080)
Trypsin/EDTA (Invitrogen, part 25300-054)
S1P (Avanti Polar Lipids, part 860492P)
Fatty Acid Free BSA (Calbiochem, part NC9734015)
Zeocin (Invitrogen, part R250-01)
Hygromycin (Invitrogen, part 10687-010)
Geneticin (Invitrogen, part 10131-027)
384-well plates (Greiner, part 788092)
T175 tissue culture flasks (Corning, part 431080)
All data reported were normalized on a per-plate basis. In this assay, S1P had a 50% effective concentration (EC50) of approximately 50 nM. Possible artifacts of this assay can include, but are not limited to: dust or lint located in or on wells of the microtiter plate, compounds that modulate beta-arrestin or BLA activity, and compounds that quench or emit fluorescence.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Format: Cell-based
Assay Cell Type: U-2 OS
From ChEMBL:
Assay Format: Cell-based
Assay Type: Functional
Result Definitions
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: U01 AI074564
Data Table (Concise)


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Did not know where to put this but since it say's theme section i'll place here. All the Dynamic Themes that I have found to share with all.
Well it took me a while but finally got it. And Now i want share it with all of you so you dont pass from the same searching around i did.
PS. I did not make the theme's thank the creator's not me.
Here's The Link: http://www.megaupload.com/?d=QCEXOQXZ


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Large Teardrop Standing Aerium
A beautiful, fully planted aerium delivered to your door - the perfect Living Gift™.
This large aerium is a unique offering from Living Gifts™, and arrives fully planted with an exotic tillandsia plant.
The elegant teardrop form can be displayed on a shelf or desk, or as hanging décor. The delightful adornments are easy to maintain too – simply spritz a small amount of water on the plants each week. These lovely pieces make a perfect gift for friends, family or even co-workers.
Each aerium measures approximately 7.5 inches tall and about 3.5 inches in diameter. Materials: glassware, tillandsia, river rock.
Dimensions: 3.5" L x 3.5" W x 7.5" H
Weight: 0.5 lbs.
Large Teardrop Standing Aerium
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- Three-Legged Tale
6:00PM to 8:00PM
Experience the creative puppet production Three-Legged Tale, a story told without words. In the middle of the forest, a squirrel digs through a garbage pile and finds a camera on a tripod. When a butterfly lands on the camera, it suddenly springs to life, venturing forth to explore the world around it. Join us before the show for some fun activities from the Wonders of Water Brownie Journey. Who: Girls in grades 2-3 Date: Monday, April 22, 2013 Location: Sangamon Auditorium, Springfield Time: 6:00 p.m. - 8:00 p.m. Fee: $16 per girl, $14 per adult Min/Max: 15/100 participants Closing Date: April 8, 2013 Fun patch included. ...


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Education and Humanities
- Contents of This Section:
- Associate in Arts
- Certificate in Workforce Skills
- Child Development Program
- Distinguished Performance Series
- Education Program
- English Department
- Faculty & Staff Directory
- Multi-Disciplinary Studies
Welcome to the Education Division. We have a variety of certificates and degree options as diverse as our personnel. Between our twenty faculty members and three support personnel, someone is always available to welcome you and answer any questions you may have regarding our division's programs and courses.
The Bachelor of Arts in Elementary Education Degree program emphasizes the practical model. This model focuses on the theoretical, development, and technological aspects of the knowledge base that are prerequisites to the daily demands required to be a professional educator in a school environment.
The Bachelor of Applied Science in Child Development Degree is part of a career ladder program that begins with a Certificate in Child Development and is continued with an Associates of Applied Science in Child Development.
Students earning either a Regents Bachelor of Arts or Multi-Disciplinary Studies degree may wish to focus on the liberal arts. The Education and Humanities Division offers a variety of art, music, literature, and creative writing classes that allow students to explore and hone their creative talents.
The Associate in Arts degree is designed for transfer to other colleges and universities. Students who wish to earn a Bachelor of Arts or Bachelor of Science Degree at another institution should register for this degree and consult with an advisor in their chosen field of study.
Dr. David Lancaster
Education Division Chair
Why WVU Parkersburg?
WVU Parkersburg's Education Program is based on the premise that the best place for preservice teachers to learn about teaching is in public school classrooms. For this reason, the field experiences component of the program requires preservice teachers to begin working in classrooms from their first education course during the freshman year. As the student works through the coursework associated with the program, more and more time is spent in the public school.
Programs of Study
Degree Certificates
Certificate in Child Development
Certificate of Applied Science in Workforce Skills
Paraprofessional Studies Certificate
Associate's Degrees
Associate of Applied Science in Child Development
Associate in Arts Degree
Bachelor's Degrees
Bachelor of Applied Science in Child Development
Bachelor Of Arts in Elementary Education K-6 Multi-Subjects
Add-on endorsements in:
- Science 5-9
- Social Studies 5-9
- English 5-9
- Math 5-Algebra I
- Pre-K – K
Faculty & Staff Directory
WVU Parkersburg's professional education unit is accredited by the National Council for Accreditation of Teacher Education.


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About This Video
A clip/trailer of SkyfallTags : Skyfall
Type : Movie Trailer
Views : 499
This Movie Info
Release Date :November 09, 2012
Studio :Columbia Pictures
Director :Sam Mendes
Starring :Daniel Craig, Javier Bardem, Berenice Marlohe, Judi Dench
Daniel Craig is back as James Bond 007 in Skyfall, the 23rd adventure in the longest-running film franchise of all time. In Skyfall, Bond's loyalty to M (Judi Dench) is tested as her past comes back to haunt her. As MI6 comes under attack, 007 must track down and destroy the threat, no matter how personal the cost.
When Bond's latest assignment goes gravely wrong and agents around the world are exposed, MI6 is attacked forcing M to relocate the agency. These events cause her authority and position to be challenged by Gareth Mallory (Ralph Fiennes), the new Chairman of the Intelligence and Security Committee. With MI6 now compromised from both inside and out, M is left with one ally she can trust: Bond. 007 takes to the shadows - aided only by field agent, Eve (Naomie Harris) - following a trail to the mysterious Silva (Javier Bardem), whose lethal and hidden motives have yet to reveal themselves.


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The Community of Disc Golfers and About All Things Disc Golf
February 4, 2012 all day – Deming Park - Terre Haute, IN Random draw doubles 1st round and then redraw for 2nd round. Everyone will have their own individual score from the 2 rounds. Organized by Dan Wilkinson | Type: fundraiser, fo, 14th, &, chestnut, community, center
© 2015 Created by Terry "the Pirate" Calhoun.


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First! AWESOME. I love BBM. BBM w/ Voice is greater.
Good to see love still coming to BB7 while we wait for BB10.
Hot off the announcement of worldwide BlackBerry 10 events in January, RIM has introduced an awesome new version of BBM today. BBM version 7 includes some great updated features including the new BBM Voice. BBM Voice lets you instantly initiate a voice call with BBM friends for free over a Wi-Fii connection.
In any BBM conversation, simply tap the call button and you'll start a voice call - it's that simple. BBM Voice will add some great depth to BBM, providing you the ability to escalate a chat to a voice call when the situation calls for it. This feature should be huge in emerging markets or for anyone calling long distance as there will be no long-distance charges.
Additionally, in BBM you'll be able to see each users status as well so you'll know who is available for a call and who isn't. When on a call you'll see a split-screen view and can still use any other apps on your device. So you can still use the chat function as well as send pictures within BBM or even check email or use the browser.
BBM Voice is included in BBM version 7 which is available in the BlackBerry Beta Zone. If you don't have a Beta Zone account you can register for one from the link below. Don't worry if you're not seeing it yet, it'll be along soon. We'll have plenty more on all the features of BBM 7 soon so stay tuned. Keep reading for the full press release.
Check out more in the BlackBerry Beta Zone
WATERLOO, ON - For the times when you want to hear a friend's laughter instead of reading LOL, BlackBerry® Messenger (BBMTM) is taking the social networking experience to a whole new level with an exciting new feature called BBM Voice. Research In Motion (RIM) (NASDAQ: RIMM; TSX: RIM) today announced BBM version 7, a free update for the globally popular mobile social network, which will allow customers to make free voice calls to other BBM customers in the world over a Wi-Fi® connection*.
BBM is a fast, convenient and inexpensive service for BlackBerry® customers that gives them the ability to instantly chat with a friend, family member or colleague, and know when their message has been delivered, and read. With the new version of BBM, customers have a new option - they can talk with their BBM contact across the globe for free over a Wi-Fi connection. In an instant, customers can switch between texting and talking, or talk and text at the same time. For consumers, especially in developing markets, the BBM Voice feature offers an attractive way to talk with friends and family near and far, and staying in contact will be easier than ever as the holiday season approaches. For business customers, BBM Voice allows for enriched collaboration.
"BBM is the quick, easy way to stay connected to the people that matter to you, and with today's announcement we're expanding the capabilities of the service for deeper social engagement and even greater collaboration, beginning with voice functionality," said T.A. McCann, Vice President of BBM and Social Communities at RIM. "The new BBM Voice feature is for when you're travelling and you want to call home without worrying about phone charges. It's for when you have news you can't wait to share, for quickly explaining complicated details, and collaborating with colleagues. Whenever your ideas are too big for text, there's BBM Voice."
BBM Voice includes a number of convenient features.
Another important feature of BBM version 7 is its integration with a customer's BlackBerry ID, which is designed to back up their BBM profile, groups and contacts so they can automatically be restored in the event they switch to a new BlackBerry smartphone, including to a BlackBerry® 10 device.
BBM version 7 with the new BBM Voice feature is currently available in Beta for BlackBerry smartphones running the BlackBerry® 6 OS or higher with support planned for smartphones running the BlackBerry 5 OS at a future date. The Beta can be downloaded for free from BlackBerry Beta Zone**. Customers can sign up to BlackBerry Beta Zone for free at www.BlackBerry.com/betazone
For more information about BBM version 7 and BBM Voice, please visit www.BlackBerry.com/betazone.
* BBM requires a BlackBerry service plan. The BBM Voice feature requires a Wi-Fi connection with access to the Internet and may not be available in all markets.
** BlackBerry Beta Zone is not available in all markets.
First! AWESOME. I love BBM. BBM w/ Voice is greater.
Good to see love still coming to BB7 while we wait for BB10.
Yes it has
Indeed, it's all clear now
Awesome feature but I have the torch 9850 ever since the 7.1 update my speaker phone dramatically lost volume in fact its useless. They are not working on an update as I have experienced this issue for 9months now. I hate that they did that to us customers. No fix and i keep blogging and calling them about it. smh
I would say, finally they opened the feature.
I don't know when BB phones got support for the UMA and MVS services, but the feature was available on the hardware though not accessible since many years.
They should have made it accessible many years ago, and maybe even should have added support for standard voip/sip calls, but they prefer to keep it bundled to the full BES and MVS and their costs.
I want to see this love coming also to BB OS5 and even older phones (weren't the first Pearls UMA capable?), to see the potential of the hardware we bought, even if years late. RIM indeed was stupidly managed.
The Nokie E series phones had a sip client available in the basic OS, that only few cared to understand what it was for and used it, BB phones had the opportunity to have voip calls since the Pearl (if I'm not mistaken) and just imagine how huge the BBM "social network" would be if it was _very easy_ to _talk_ internationally with it cost free since 2008 or so?!
As much as I agree with almost everything you said (bringing some BES into BIS specially), supporting pre-OS5 devices right now would not help RIM any. The OS and hardware is phased out and the experience would not be good by today's standards.
But yeah, MVS back in 2008 would probably have done wonders to RIM (provided it didn't possible carriers off).
I believe UMA support is carrier dependent. Several years ago, on T-Mobile in the US, I had a $10 plan that allowed unlimited UMA calling. It would switch to the GSM network automatically if I left my hotspot w/no additional charge when the call was initiated on UMA.
I've been on Verizon Wireless for the past 3 years, and they don't support UMA.
BUT...this enhancement is AWESOME!! I'm rebooting my Bold now!! :)
with this feature you will be able to voice chat not only BB7 friends but BB10(when released) friends as well. This is actually fantastic.
Skype would be better, then you can voicecall ANYONE not just people with BBs!
Dnag! Was almost first! Was too busy thanking JCarty for the BB10 Timer and missed it! Oh well...2nd and YES! Now I need some more BBM friends back on board!
And missed 3rd posting to this! Ugh!
Considering that this feature only available through WiFi, I don't think there is any limitation towards AT&T or other carrier.
Just like the FaceTime at the first time.
Perhaps, later, when they allow it on 3G/4G connection, we will see AT&T trying to 'save' the day.
Lmao!! Classic!
That's useful for me, I'm on work trips all the time. Now it will be free to call family :)
i have to say that's pretty genius. finally i found a use for bbm lol.
me either. :(
I bet you are right!!!
Sounds cool to me but what about BBM video chat? There were rumours about that as well but is there any confirmations or details about that?
Great feature to have and it only signals what we can expect out of BBM on BB10, though with the implications that it has for BlackBerry users, especially in emerging countries, I wish they had developed this sooner. It would have certainly quelled some of the rumble about Skype being absent on BlackBerry (and possibly helped retain a lot of former BB users).
Yep, at first I thought this was cool but then it dawned on me that other Phones have video calls so I felt It lost the wow factor.
Plus T-Mobile UMA or wifi calling been using that since 2008 so no big deal. The only cool thing is you could use it as free calling to international users of BBM.
Max... Show me a single BlackBerry smartphone bing sold today that can actually make use of video chat apps.
This is great news and it is not the same a saber UMA calling. UMA just routes the call over WiFi when you have poor to none carrier coverage (I have no idea if it is free or billable though - no carrier around here bothered to deploy UMA - so feel free to tell me).
This is as close to true VoIP calls as the old java platform based ever been before. Now we have a in-house free app for free calling each other. If BB7 phones had front facing cameras, I am willing to bet we would have seen BBM Video as a feature of BBM7.
But heck, what do I know...
although this is clearly great for BBM, but this is not exactly groundbreaking technology.
Apple has it with its facetime
Android has it with Gtalk/G+
Cross platform Apple/Android has Skype
Of course, should BB support Skype app, we should not be left in the dark in this free VoIP.
I am excited about this as it supports OS6 (so my 9800 can use this feature), but not so much considring RIM is so far behind in innovation
Stop being a debbydowner.
Actually apple stole facetime as they call it and just settled a lawsuit with the iphone. Bring on round two. http://news.cnet.com/8301-13579_3-57547606-37/in-your-facetime-apple-get...
RIM is far from behind in innovation, the legacy os has been the downfall of Rim. Soon the turn around will begin.
Excuse me? "should BB support Skype app"? Really?
So is it RIM's fault now that we have no Skype app? Do you have a copy of the letter where they tell Skype to can the beta app they never release?
Seriously, this was one weak argument.
You are right, other platforms have such features. Now BlackBerry does too. I fail to see fail on this.
It is pretty cool. I just wish it worked over cellular, then it would be awesome. That would be a great way to get people on BB again if it worked over cellular. They could advertise that you can now use your BB to talk without using any minutes at all to any other BB user in the world. I doubt any carriers would RIM do so though.
If RIM does to VOIP what they did to messaging, than this will be a game changer. A green light indicating that the person is there and will likely answer you with either text or voice, holy smoke!
Using BBM to bridge the gap between the new and old platforms, yes, I believe in this too.
As for skype. It changes nothing. We're talking BBM, which is exclusive for BlackBerry devices. Skype is for cross-platform communication (IM/voice/video).
BBM Voice/Video does not prevent not denies the existence (and necessity) of a Skype app.
The most likely approach from MS would be to drop specific apps and introduce a web-based client that would work for bb10 and the other two while keeping a native app version as a perk of wp8 devices, that way they don't reduce the potential audience but do increase the benefits of wp8.
Works great!
This is FREAKING GREAT!!! let's talk TEAMBlackberry, let FaceTime buddies out there realize that 80 million BB users can also do what they can do. GO RIM!
I can't wait to test the BBM 7 beta, downloading now! can't wait for the official release to come so I can have BBM voice to all my BB friends wherever they are!
** dunno why but.. it doesn't appear on the Beta Zone yet, anyone knows why??
Seems like an awesome addition to the bbm software.. i wish they made it run on normal data as i have 6 gb's of data and i never use passed 2 gb. i would really like them to allow us to use our carrier data. noww that would be freakin awesome buh either way i love the way RIM is going right now. lets rock and roll this!!!
I Smell videochat availability on the BB10 TouchScreen.
Hopefully the keyboard version would have front camera already.
The Start of Something UNUSUAL BB10
As long as the LTE bb10 can do this without wifi I am happy. And video chat
Top dank voor deze tip, ik heb hem draaien!
Now this is good news! I've got people all over the word I need to chat with, most definitely will have people in that category jumping unto the platform
Can any one send me the link to download this bbm voice ?? Because when i login my blackberry beta zon i cant find this program ......
my email is email@example.com
brilliant.. nice thing to have in BBM. But i feel carriers will play spoil sports in this. Imagine wat loss of revenue it will be for them if such a feature is available in ur BBM. they will somehow charge u for it.. lets hope n cross the finger carriers dont interfere in it.
Don't mis interpret this post. I'm a big blackberry fan. I always buy the newest phone the day it comes out... However, I have never used BBM and I don't see a point in it. I never use all of my minutes, and I don't make international calls. This is true of most smartphone owners. What does BBM do that I can't do without it?
Great move by the NEW RIM to add to the BBM community with this voice feature. Too bad the OLD RIM did not see the need to add front facing cams to the BB7 hardware. I believe this oversight lead to the loss of many more BlackBerry users in North America. BBM Voice will help get some back.
This is good news.
There are still parts in the UK where using a mobile is still not good.
I have been using Viber on an old "android" and will be really pleased if I am able to use my Blackberry instead to keep in-touch with some of my friends. I hope this will be as good as Viber is.
Just, hope they release this "upgrade" in the UK.
Because 1. It won't let me log in saying it's a bad request. 2. From what I'm reading it's no even in there. (In the UK at least.).
Excellent! RIM back in business! :P
So I get that the chat will take place over Wifi, but will you need a BIS cellular connection present to be on BBM in the first place?
Will this work at a motel up north that has Wifi but no cell service?
Will it work in a foreign country where I have Wifi but no data roaming?
If the answer is no, I would rather have something that is independent of BIS.
Fantastic news! So you have tried it and can confirm it on that basis?
Makes sense. Thanks for confirming.
BBM Version 7 is live on BlackBery Beta Zone.
Not to sound like a downer, but can someone genuinely tell me how this is a big deal if we've already had UMA / wi-fi calling on our devices for 5+ years? What's the difference between this and wifi calling / uma?
The only difference I see here is that its integrated within the app itself rather than the user needing to exit out and dial the number (when on UMA) which while nice, is hardly the earth shattering moment some are making it out to be...
I think it is because not all country have UMA feature, like here in Jakarta Indonesia. This BBM Voice would be very welcome here.
Kinda like comparing skype voice to UMA calling.
UMA calling is not available on all carriers and even on carriers it's available on, it may still cost you if it's not factored into your monthly plan (try making an international UMA call and see if you don't get a separate bill for it). UMA still basically still makes use of the carriers infrastructure
BBM voice on the other hand is VOIP which is not carrier specific and if RIM chooses to do so can make use of the already existing data plan on the device.
Only real similarity right now is wifi because really, they are 2 separate concepts
Thanks for the answer.
BTW - I've used UMA when traveling internationally for over 5 years now in some 15+ countries and have never been billed (meaning roaming and long distance) for any of those calls.
Obviously the minutes come out of your monthly bucket, but other than that, no charge.
Either way, interesting feature that I look forward to using.
You are welcome. :)
You are right with that aspect of the UMA billing however, if your home network was say USA and you called UK via UMA, (unless free international calling was included in your plan) you could see a separate bill for it like i did.
I got a rude awakening when i saw my bill after placing quite a few phone calls to the UK via UMA on the Tmobile USA network.
Now when i was placing calls to my home network (USA) via UMA, i got no separate charges regardless of what country i was located in.
UMA is the reason I have stuck with TMO and BB for so long. It really is the best thing ever and everyone is so jealous of this feature that no one really knows about.
It will break my heart if BB10 doesn't have UMA or WiFi calling.
This is awesome, hopefully it'll keep existing bb users on board and bring back old users as well. I think it's a great plan, it'll soften up the blow for the existing users who are locked in their existing plans but on older bb phones but at the same time keep the communication lines open for those who will be buying the new BBX. I just have one question, because the update now will tie into your bbid, does that mean that PB users will eventually get it as well? I am not a bb user, but am a pb user. I never grew to support the older bb phones hence why I use another phone. But with the introduction of QNX, I really look forward to BBX since my contract is expiring early 2013. I just hope they put alot of effort into the aesthetics of the phone as much as they have worked hard with the OS. The image of the BBX next to the iphone 5 comparison makes the phone a bit bland. I'm sure with the OS on, it'll rock for sure. But I guess I was hoping that it will stand out of the crowd a little more. Maybe like the Bold that has the aluminum strip wrapped around it or something that will catch people's eye. Remember, it's one thing to have a great OS, but there's a lot of people who buy simply by aesthetics as well. So having both will ensure a great outcome for BBX. I prefer to have both if possible.
Finally got the invitation :) Horray. You guys should be receiving one anytime soon now too..! (If not already..)
My thoughts exactly!
Awesome!... now i just need some bbm contacts.
Will the person I am chatting with be able to talk if they have not downloaded this beta version? My friends shy away from beta versions until the real thing comes out...
Tonyrón - One Day at a Time
Wow, thats a good surprise from RIM, a very good move to maintain it's current user base, specially the enterprise who really can lower it's cost on calls.
BBM is not Skype, but would be a greate addition for those BBM fans, and also a good way to unite BB10 users with lower versions.
I am not sure how to express or quantify the level of excitement to try this at this point.
BB 9900. / 7.1 bundle 2107 (v18.104.22.1686 platform 188.8.131.526)
Montreal Canada
Bell Mobility
Available right now
up & running on my phone
had to update my BBID also
cannot find the new emoticons though??
Is BBM going to start dipping in the SKYPE business??
having problems entering keycode. Any tips?
I had to try three times until it finally accepted my keycode.
Watch for spaces at the beginning and end of the keycode
Geez thanks for the tip!
At first I wondered if the WIFI limitation is a technical thing or a "let's not piss off the carriers right before we launch our new platform when we'll need their support in promoting us" thing.... My thoughts being that VoIP eats at the carriers' minute plan sales.... but then they still sell data plans so the carriers still get sales, but I think they make more by selling you 2 items over 1 (minutes and data vs just data).... Eventually I wish they could do away with this minutes and data combo and just sell data, with everything being data. (not sure on the technical hurdles for full VoIP wireless service, but the idea buying a chunk of data and using it as you wish seems nice) In that case you'd buy x amount of data, with an equivalency of how many minutes that data allows you, based on the sound quality you choose. Just my take.
Absolutely downloading right now. Excellent move RIM!!! Keep the hits coming. Please and THANKS!
Proud owner of a BlackBerry Playbook & Torch
I tried to create a beta zone account but was given "application form for your country is not yet available" message, and I'm in Canada. Any ideas fellow CB fans?..
Tried a few more times and keep getting:
"We're sorry, the application form is not yet available for your country. We are adding new country locations over time. Please be patient as we continue to expand across the globe."
So I guess they've changed it up a bit or something.. :(
I'm 30 mins away from RIM dammit lol. Ok thx for the heads-up, I will keep trying!
Your IP is coming from Canada?. Your not using something like Hotspot Shield etc?. I'm 30 minutes from RIM to and everythings tickety boo!! :-)
Nope, trying right from my 9900. Maybe I need to try from a PC...
All good now, registered and downloaded...now I guess I need contacts with the same version so I can actually try it out! 2908CA52
love it...
Great new feature for emerging markets
It has frozen my 9800 torch on OS v6...trying to recover now!
Backed up on the desktop. Deleted app world, and have now got BBM voice registered but there are no new icons...maybe the other person has to have it loaded as well?
Still got not app world running though due to this new version not displaying on hardware running OS6....
I think BBM on BB10 will be BBM10
Just tried it with a friend (both of us are in Toronto) and it works wonderfully.
Can't call if not on WIFI (as it says). Just says to turn on wifi. Also can't call if you or the person you are calling status is set to Busy (even personal statuses with Busy selected)
But if both parties are available and both have wifi on, the Call icon on the top right of the chat window turns green. Click it and away you go!
Audio is a bit delayed (i'd say 3-5 seconds) but that could just be due to our wifi connection speeds.
now downloading to try it out!!
This makes me wish i had contacts in BBM
For all those wondering what new countries have been added to the BlackBerry Beta Zone, here is a list of the new country additions for November:
BlackBerry Beta Zone Expands!
We are pleased to announce that BlackBerry Beta Zone is now available in the following regions:
American Samoa
British Virgin Islands
Isle of Man
New Zealand
Northern Ireland
Papua New Guinea
Saint Martin
Sierra Leone
United States Virgin Islands
Not sure which ones were already included but I would assume all North American countries and most if not all EU countries are.
I need a beta tester.....let test BBM Voice
I uninstalled BBM 2 years ago. No one I know has a BB. They've moved on.
*ACTION starts here.
more to come RIM.
Something UNUSUAL BB10
OH come on RIM, you tease. WIFI only?
Because the carriers would have a fit if RIM made it work on the data stream. In fact the carriers would demand RIM to build in features that would allow them to block the voice call feature forcing you to pay them an extra fee to have that service enabled.
It would be nice to see BBM 7 for the playbook as well. :o)
When in log in om my bèta account in fontein see any download url forum bbm7 RIM says in acht email that it van taken up 24hours.....
Any other users in Netherlands that have this issue???
Don't Hate Me Because I'm Beautiful.
Blackberry Storm2 OS5
BlackBerry Bold 9780 Black OS6
BlackBerry Bold 9700 White OS6
BlackBerry PlayBook OS2
BlackBerry Repair Center Amsterdam
Geen last van
Broke my text messaging contacts! Won't open them (only opens bbm contacts), even after deleting and re-adding them, and rebooting the phone (9810 w/188.8.131.526). I had to roll back to 22.214.171.124 because I use BBM for my frequent SMS contacts as well.
When can we expect a version over data and not wifi?
Never unless you want your carrier to start charging you more for your services. :o)
Just a question: does both parties have to be on wifi or just the caller???
Its working perfectly well in Nigeria. Called Canada and the UK and there were no problems. But i just wonna ask can this in any way be used to connect to the playbook. Just asking. Okbye
Hey, yep I can relate. Shoot me a PM and we'll give it a try! 2908CA52 for anyone else wanting to try some beta trials.
I need someone using BBM and that is a beta tester of the BBM 7 with voice. I saw the video, but would like to try it out for myself to complete the follow-up survey. None of my friends have their BB anymore so if anyone is available for testing please PM your PIN. About to complete the survey soon on this beta.


text:
Miguel Cabrera in Nominee for Hank Aaron Award
Major League Baseball and MLB Advanced Media announced that Miguel Cabrera was named the Tigers nominee for the 2013 Hank Aaron Award. Full story »
2014 ¡Fiesta Tigres!
Tigers players and fans enjoy this annual celebration of hispanic heritage at Comerica Park.
Launch Gallery »
Miggy wins second straight American League MVP Award
Miggy celebrates winning MVP with his family
ATL@DET: Anibal strikes out 17 to set new Tigers mark
DET@CWS: Iglesias retires Phegley on diving throw
KC@DET: Miggy's homer gives Tigers walk-off win
WS2012 Gm3: Miggy receives trophy for Triple Crown
OAK@DET: Ordonez's AL Pennant-clinching homer
SD@DET: Hernandez closes out the World Series


text:
- Theodore A. Hoppe
- Montpelier, VT
- United States
This conversation is closed.
Do you watch TEDxTalks that aren't posted at TED?
TED does its best to bring us the best of the best TEDxTalks,
Have you seen the list of TEDx events that people are holding all around the world?
There are so many excellent speakers, and talks, that for one reason or another will never be added to this site but do get posted on YouTube Many TEDx events are streamed on the internet live, other get taped and then uploaded to YouTube. I have watched over a dozen live TEDx events and have also watched over 100 TEDx videos.
Who else watches these? Do you have a favorite talk that you would like other to watch? Post a link here and tell us why we should watch it.
Feb 4 2013: www.youtube.com/watch?v=aAox9e1cpsQ is the link to the TEDx poem I wrote and preformed as a 17 year-old. One of the proudest moments of my life so far, the poem is about my frustration of how we, as humans, can't get along
Feb 21 2013: Hi Kieran, really wonderful performance.
- 0
Proud moment indeed!!!
Let's hope one day soon you will write the poem about how we humans are finally getting along.....Feb 20 2013: To make real progress in improving the health of the underserved, the poor, and the homeless, our doctors must do more than diagnose and treat patients. Ali Arastu believes a physician's role is to help shape a society where illness and disease is less rampant by understanding the full needs of the communities they service. Officially he is a student at the Keck School of Medicine at USC but he also has chosen a unique way to be a student of other determinants of health, like poverty and our environment. Whether he was on a five-month journey on foot along the Pacific Crest Trail or living on Skid Row, he brought back stories that are having a profound impact on his journey through medicine and life.
Feb 21 2013: This was very enjoyable to watch....thank you for sharing.Feb 20 2013: Deanna Choi is a violinist and an undergraduate psychology student at Queen's University. Her talk mixes live performance with a presentation on the psychological benefits of music.
- 0
http://www.youtube.com/watch?v=XZFKpkDUMB4Feb 4 2013: Here are two of my favorite TEDx talks not on TED.
Humans Seeing Humans: How the Brain Perceives Other Minds" shows that despite the realistic human characteristics displayed in the faces of dolls, computer animations, or other inanimate objects, Dartmouth Assistant Professor of Psychological and Brain Sciences Thalia Wheatley shows how her recent research proved that most details are irrelevant since there is only one feature that is a true window to a soul.
Hannah Fry trained as a mathematician, and completed her PhD in fluid dynamics in early 2011. After a brief period working as an aerodynamicist in the motorsport industry, she came back to UCL to work on a major interdisciplinary project in complexity science. The project spans several departments, including Mathematics and the Centre for Advanced Spatial Analysis, and focuses on understanding global social systems -- such as Trade, Migration and Security. Hannah's research interests revolve around creating new mathematical techniques to study these systems, with recent work including studies of the London Riots and Consumer Behaviour.Feb 2 2013: I took a quick peek at the group of TEDx talks recently posted on theCity2.0 site. As a pressing interest of mine is improving the quality of life in cities, I am delighted at the wealth of experience thoughtful, action-oriented people are sharing there. I just need to decide on my strategy for going through those talks in a way that avoids eye strain and overload!
Dan F
Feb 2 2013: Hi Theodore,
- 0
TED offers a standing invitation and opportunity for each of us to participate in an open aired mass media production and exchange of views to influence one another. A nice example of democracy at work.
Obviously there is a screening process in being part of the show. You must be civil in expressing yourself and If you can pass the review process you can walk out on stage with a world wide audience to present an idea considered by TED to be worth spreading.
This type of enterprise will always produce critics. I'm glad to see that TED allows their voices to be heard as well.
I do have a suggestion for TED. I would like to see them offer a one time single discounted ticket to a main featured TED production for individuals who have earned a TEDCRED score exceeding 100. It may well prove to be a net gain as an incentive for membership participation and who knows might even liven up the event!
If it happens -- you owe me!Jan 31 2013: This is the next TEDx event that will be streamed on the internet live. on Feb. 9th
http://www.ted.com/tedx/events/6310Jan 31 2013: Do you mean another site? Or perhaps get the Ted team to give us another section on this site for links. Personally I think this is a great idea. My understanding is that not all Tedx videos make it to this main site. TED.com But I have attended the some TEDX events and i know that the events I attended have their own website and on that website they post ALL the videos. The youtube ones would also be interesting to watch.
I imagine the coding and indexing would be some work depending on how well the posting section was indexed. Do we have just a simple section to post "hey everyone look at this great TEDx talk" OR, Do you have a section like that PLUS a searchable database and highlighted links for new talks etc.
For the moment a simple posting section might suffice.
Jan 31 2013: Here is an example of one of the TEDx talks from YouTube that I was watching last night. This is the talk that inspired me to post this idea. Its by a courageous young man that gave a talk about mental illness.
Andrew Steward -- Beating Mental Illness
"When someone breaks their arm, we rush to sign their cast. When someone is diagnosed with mental illness, we run the other way."
Andrew Steward is a flute performance major at the University of Denver, excelling in school and planning a graduate education. But a chapter of his life included debilitating mental illness and tormenting hallucinations. Andrew bravely pulls mental illness out from under the rug so that we can erase the stigma and focus on helping people who are ill.
See complete bio and all TEDxDU Talks at www.tedxdu.com.
http://www.youtube.com/watch?v=JyQxQ7KaU1s by a courageous young man that gave a talk about mental illness.
Thank you TEDxDU Thank You AndrewJan 31 2013: There are 24,000 TEDx Talks on YouTude
http://www.youtube.com/user/tedxtalksJan 31 2013: i would love to attend a TEDx event. I just cant afford to go and being active in the military prevents me from traveling to see a live event. but i LOVE the videos. i'ts one of the most productive ways to kill time while surfing the net.
THANKS TO ALL OF YOU for all your hard work and passion
Jan 31 2013: This is the next TEDx event that will be streamed live on the internet on Feb. 9th. TEDxAustin
Jan 31 2013: i'll be watching.
where can I see what topics will be discussed at this event?
Jan 31 2013: Check out these links on the original event link "TEDxAustin website »"
That is also where you find the link for the live feed
And you can write to the event coordinator.
Nancy Giordano
Austin, TX, United States: the link to profile and email are at the same page.


text:
Description:Intel® Xeon® X5482(3.20GHz/4-core/12MB/120W)Full Processor Option KitPart Number(s)Manufacturer Part# 495614-B21
HP Processor Kits:
Manufacturer Part# 495614-B21
This is the full processor option kit. It includes the
Processor(s), Heat-Sink(s), Fan(s), Thermal paste
and VRM (Voltage Regulator Module) where required.
Overview:Information technology professionals rely on HP quality to ensure maximum productivity. As the power of applications processors memory adapters and storage devices increases high-end computer users are seeking ways to optimize their system performance. HP provides leading solutions adding new levels of performance availability flexibility and management.
CategoryProliant Processor
Sub-CategoryIntel Xeon
Part Number495614-B21
Products ID458300
Product TypeProcessor
Processor TypeIntel Xeon
Processor CoreQuad-Core
Processor Qty1
Clock Speed3.2GHz
Bus Speed1600MHz
L1 Cache256KB
L2 Cache12MB
Process Technology45nm
Processor SocketSocket LGA-771
Thermal Design Power120W


text:
Migrants, Migration, and Development
Migrants, Migration, and Development
Recent Activity
This report provides a global look at circular migration experiences, depicts various governments' attempts at creating circular migration, evaluates the economic costs and benefits of circular migration for sending and receiving countries, identifies components of effective bilateral agreements, and reviews outcomes governments might realistically expect from their circular migration policies.
This brief takes a look at hometown associations (HTAs)—immigrant organizations based on a common hometown—and their often overlooked function as integration intermediaries in their country of destination.
The situation in Iraq remains extremely precarious for civilians, with additional thousands being displaced by violence and persecution even as refuge becomes harder to find – either internally or in neighboring countries. This report looks at the refugee situation in the country.
This report investigates how migrant-sending countries can protect their migrant workers abroad by examining the policies, functions, and challenges of the Philippine government's membership-driven welfare fund, the Overseas Workers Welfare Administration (OWWA).
This report offers an intricate look at the slowdown in remittance growth experienced by Mexico in 2007 by comparing remittance flow figures among Mexican states, and highlights regions that may be particularly vulnerable to risks associated with remittance fluctuations.
This report identifies features of remittances that make them ideal leveraging agents for poverty reduction and migration management agendas, and proposes a four-part international research and policy agenda for maximizing the development impact of international remittances.
This report explores the role of ethnic community-based organizations as drivers of refugee integration. It highlights contributions, challenges, and best practice methodologies identified through site visits to refugee-serving ECBOs across the country, and offers recommendations for enhancing the capacity and sustainability of refugee integration services.
This report captures India's exceptional growth in reported remittances over the 15-year period between 1990 and 2005. It explores the relative importance of these remittances in India's economy, and then identifies factors responsible for this exponential gain.


text:
- Independent
- The Enterprise
- The Recorder
Attorneys on behalf of the Calvert County Government and the Calvert County Fraternal Order of Police Lodge 109 presented arguments during a motion's hearing Wednesday morning about a civil injunction, filed by the FOP and three sheriff's deputies, seeking annual salary merit step increases for the past four fiscal years.
The attorney representing the county government filed a motion to dismiss the case, which was the basis for Wednesday's hearing in Calvert County Circuit Court.
The complaint for declaratory and injunctive relief and for writ of mandamus was filed Oct. 10 for all deputies of the Calvert County Sheriff's Office seeking the step increases, which are earned through longevity and satisfactory job performance, for fiscal years 2010, 2011, 2012 and 2013, that they claim have been unlawfully denied them. The civil suit also seeks to require the county to grant annual salary increases at the beginning of each fiscal year for all deputies who have received an annual performance evaluation that indicates a rating of at least satisfactory, according to court documents.
Because deputies have been denied annual step increases, they are at least four steps lower on the pay plans or salary scales than they would be had the county not violated county code, the suit alleges, adding that deputies have "suffered from reductions in their salary" by as much as 9 percent of base pay.
Attorney Victoria M. Shearer, of Karpinski, Colaresi and Karp, P.A., who represented the county government, said in the civil injunction the FOP is claiming deputies are entitled to step increases based on the section titled "step increases" of the county ordinance code titled "pay and adjustments." That section of the code reads: "Subject to the availability of funds and the terms of the pay scale, the Board of County Commissioners grants step increases once per fiscal year" to certain employees, including merit employees, who "shall be granted a step increase at the beginning of the fiscal year" if they "have received a satisfactory or better performance evaluation."
The language of the code implies that employees will be eligible for, not guaranteed or mandated to receive, step increases, Shearer said. Under Maryland law, she said, commissioners are not authorized to mandate step increases and never contracted to do so with that ordinance.
The plaintiffs assert that the language, "subject to the availability of funds," mandates a step increase, which is incorrect, she said, and it is for the commissioners to decide when the funds are available. She said the plaintiffs' arguments ignore the language of the statute of the ordinance, that the plaintiffs' interpretation of the code "surrenders" the authority of the commissioners to make legislative decisions and would make the ordinance invalid under Maryland law.
The "plaintiffs' arguments are creative," Shearer said, but are incorrect as there is nothing mandatory in the statutory language of the code "whatsoever."
Shearer said the complainant "incorrectly" alleges that the county hasn't complied with the rules of the code by refusing to provide the merit steps "as required" because funds were available to do so.
According to a press release issued by the FOP last October, for fiscal 2013, the county government projected a fund balance of $66.5 million and part of the fund balance is in an unreserved and undesignated funds account that is not restricted, committed or assigned to any use. In fiscal 2010, the fund had a balance of $17.6 million, which, over the last four years, has increased by $14 million to $31.6 million, the press release states. Thus, from these fund balances, the county had the funds available to provide deputies with step increases each year, court documents allege.
Attorney Abigail V. Carter, of Bredhoff and Kaiser, P.L.L.C., who represented the FOP, said the county created a code that would allow it to annually look at whether employees were eligible to be compensated and, if they were, would receive step increases every year subject to availability of funds.
Once the budgetary process was complete, there was "nothing to indicate funds weren't available," Carter said. Carter said she is not challenging the county commissioners' budgeting skills, but if there is money left over after the budgeting process is done, step increases should be given because the county's "own ordinance requires them to" do so.
In the code, the phrase "subject to availability of funds" is a predicate, Carter said, and once that predicate is met, the step increases will happen. Carter asserted that the predicate was, in fact, met based on the county's past budgets and steps should have been given.
Shearer said it's up to the commissioners, not the court, to determine if funds are available. She said the court could not accept the plaintiffs' argument without "invalidating" the ordinance.
Carter disagreed, stating, "It's an exercise of discretion" by the county commissioners.
Retired Prince George's County Circuit Court Judge Graydon McKee said he needed to further review evidence and would make a ruling as to whether to dismiss the case at a later date.


text:
If you're in the neighborhood, stop by the WNUR Streetbeat showcase at Morseland (1218 W Morse) tomorrow night. Featuring Streetbeat DJs Searchl1te, Jeekoos, Deluge, D-Vo, and Behind These Eyes. 21+, $5 at the door. 9 PM – 2 AM. Check out more info on Facebook.
Here's another show you might've missed in its original broadcast, since it aired over our winter break on December 23. Rock Show MD Dan takes a look at Nigerian musician William Onyeabor, and the results are predictably funky. And make sure to check out our Mixcloud page, too, where you can find a bunch of our stuff archived.


text:
Hey James. I see that you do a lot of app reviews. ( Good idea ) How about if you rate them at the start of your comments ... say out of 5 stars? For example:
RATING: (3 stars) - If you are after for a new funky game for your BlackBerry PlayBook then look no further as Jump Birdy Jump is here and it's a right bobby dazzler! Developed by Ovogame, who also brought us Smiley's Pop and Tropical Mania,
.... just a suggestion.


text:
The beta of 10.10 is available now for download and upgrade by testers.
Kubuntu will release version 10.10, the Maverick Meerkat, in October 2010. With this Beta pre-release, we are introducing some new and interesting things in preparation for the next official version of Kubuntu.
A remix of our Jaunty Beta has been made with KDE 3 packages. The main focus of this remix is to keep the mature, stable, and familiar KDE3.5 desktop environment available for easy installation and use. Along the way, various bugs have been fixed, and small enhancements added, see the release notes page for information and where to download.
Kubuntu 8.10 Beta has been released. This is a major upgrade for your desktop featuring the beautiful KDE 4. Read the announcement for more information and where to download or go straight to the upgrade instructions.


text:
Product Details
Sorry, this product is not currently available.
If you would like to take advantage of our Loyalty points scheme then you will need to create an account. It is a simple account setup and this helps you track previous orders and take advantage of the Loyalty Points Scheme
We accept NapNap vouchers!
Go Real Member
FCNR Member
QR code for mailing list
Sorry, this product is not currently available.


text:
Practice Philosophy
As Plastic Surgeons, we are the leaders in the field regarding non-surgical and surgical procedures. With so many options available to us, the goal is to find the right options for you as an individual. Safety, a natural appearance, and trying to obtain realistic expectations in a thoughtful and caring atmosphere is the goal.
I am lucky to have great partners and an awesome staff. Once you walk in the door you will be part of the family. Your concerns will be treated with respect and time will be given to assure that the appropriate treatment plan is mutually decided upon and initiated.
We look forward to have the opportunity to see you. We'll do the best we can to make every visit a pleasant one.
Areas of specialty include procedures listed to the left
Many procedures can be performed as an outpatient, if preferred, at our fully accredited and state of the art surgical center located in the Oaklands Corporate Center, Exton, PA. The Surgery Center of Chester County can be reached at 610-594-8900.
Location & Office Hours
Suite 110
460 Creamery Way
Exton, PA 19341-2533
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Payment Options
Medicare, Credit Cards, HMO/PPO/Other Insurance