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Comic 249
Link: Comic 249
Translations: http://www.beaverandsteve.com/wiki/images/8/8f/Finnishflag.png Finnish, http://www.beaverandsteve.com/wiki/images/5/52/Frenchflag.png French, http://www.beaverandsteve.com/wiki/images/5/57/Polishflag.png Polish, http://www.beaverandsteve.com/wiki/images/0/04/Danishflag.png Danish, http://www.beaverandsteve.com/wiki/images/7/79/Italianflag.gif Italian
Title: 'snow joke - part 5
Date: January 2, 2007
Summary: Intestinal gift exchanges lead to an unexpected esacpe from the snowman.
Onomatopoeias used: "SWOOSH", "HISS", "CRACKLE", "BLOIK", "SPLOOSH", "TWITCH"
"Fin" style: Snowmen, wins hats scarfs and corncob pipes
Number of panels: 10
Panel 1
Beaver, Steve and the Christmas tree lay slumped in the belly of the snowman.
Panel 2
Beaver: "Oh well, at least we can still open our Christmas presents! Here's one for you!"
Steve: "Oh! Presents!"
Panel 3
Steve: "Oh! Grit! How did you know?"
Beaver: "Let's just say a little bird told me..."
Panel 4
2 Weeks Earlier...
Beaver: "Hmmm...." While reading Steve's wishlist on Amazon.co.uk
Panel 5
Steve: "WEE HEE HEE HEE HEE!" While SMOOSHing the open bucket of grit into the air.
Beaver: "Careful Steve! You'll spill it!"
Panel 6
The dropped bucket of grit HISSes and CRACKLEs on the ground.
Beaver: "See Steve, this is why you can't have nice things."
Steve: "Oop."
Panel 7
Snowman: "Oh dear..." While holding his BLOIKing belly.
Panel 8
Snowman: "I'm meltiiiing!" As his belly ruptures with a SPLOOOSH and sends Beaver and Steve flying out in a jet of water.
Panel 9
Beaver: "We did it! We're free! Christmas is saved!"
Steve: "Yay! Mincepies all round!"
Snowman: "Bah, Humbug"
Panel 10
Steve notices his TWITCHing snowman on the ground, apparently having come out of Beaver's snowman's belly.
Steve: "And look! My snowman came alive too! This is the best Christmas ever!"
Steve's Snowman: "Kiiill meee..."
Steve: "Merry Christmas Everyone!"
Fun Facts
- Grit happens to be minute rough granules, as of sand or stone.
Spreading grit on snow is a common practice to stop from slipping - though it's not known for it's melting abilities.
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Polo with drop opening, stripes piping on back cut, shoulder and drop opening.
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Stop poor currency circulation
Counterfeit money has been around as since the very beginning of the use of coined currency in ancient times. Despite the harsh penalties imposed on counterfeiters by governments throughout recorded history, the crime of counterfeiting has flourished worldwide. In fact, in the United States, the crime of counterfeiting money is on the increase. The United States Secret Service seized over $88 million in counterfeit money in the fiscal year covering the time between 2013 and 2014, which was an increase over the previous fiscal year. It is important to remember that this amount represents only the amount of counterfeit money that the Secret Service knew about and seized. There are countless millions of undetected counterfeit dollars still in circulation.
How Can I Protect My Business?
All businesses large and small are threatened by counterfeiters. Whether you are running a small mom and pop type store or a large volume retail enterprise, your business is equally susceptible to losses encountered due to receiving counterfeit money. When your business takes in counterfeit money, you take a loss for that amount of money; period. Fortunately, you do not have to be at the mercy of counterfeiters. Today, due to advances in technology, you have a wide variety of affordable counterfeit detectors to protect you from financial losses due to counterfeiters.
Counterfeit Money Detectors
When shopping for a counterfeit detectors, you need to make sure that you get a counterfeit detector that meets your business's needs and budget. The type of detection device you need depends upon the type of business that you are operating and the volume of cash that passes through your business. There is a counterfeit detection device available to meet the needs of any business.
Small businesses such as convenience stores that deal in large amounts of relatively small cash transactions may be able to use a pen type counterfeit detector. These pen type counterfeit detectors allow cashiers to quickly scan money while the transaction is still taking place. This has two advantages: It allows the cashier to identify the phony money before the transaction is completed, and it allows the cashier to identify the potential counterfeiter and alert the authorities.
Larger businesses can buy counterfeit detection devices that are designed to process and check large amounts of cash for authenticity. These types of counterfeit detection devices are capable of quickly checking stacks bills of assorted denominations to ensure that they are legitimate.


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Hang tight!
Recipe by Dorothy
"This is a soup I have come up with myself that everyone in my house will eat."
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1 pound
dried split peas
10 cups
1 pound
smoked sausage of your choice, sliced
5 cubes
chicken bouillon
1 1/2 cups
chopped carrot
1 cup
chopped celery
potatoes, peeled and chopped
1/2 teaspoon
garlic powder
1/2 teaspoon
dried oregano
bay leaves
onion, chopped
Made this last night in my extra large crock pot. If you follow the recipe exactly it makes a huge amount of soup so be sure to use a big crock pot. Husband loved it! He had three big helpings. Son (9 years old) just thought it was ok but did eat it. I diced the carrots up really small and he didn't even notice them, he hates carrots. When I make this again I will only use 8 cups of water instead of the 10 the recipe calls for. We thought the soup was a little thin and we prefer a thicker version of Split Pea Soup. Otherwise it was very yummy and I will definitely make it again! Diced ham would be good in this soup too!
My husband was the one wanting a pea soup. He didn't care for this recipe until I put the soup in my blender and pureed it. It has a very good taste, with the sausage.
544 Ratings
I made this with 16 oz cubed ham instead of sausage. EXCELLENT! I did make a few other changes after reading reviews though. I used 8 cups of chicken broth instead of the 10 cups of water. I also used fresh minced garlic instead of the garlic powder.
I only added about half of the peas in the beginning...I was getting nervous around 3 hours because it didn't seem to be cooking at all! But then about a half hour later it was really going and I added the rest of the peas, and it went for a total of about 5 hours on high. So it came out with a nice thick pea mush with some whole pieces too.
I was a little nervous about the lack of a lot of "seasonings and spices" here...but trust the recipe and use a good quality pea and meat and the flavors will really come out where they are supposed to! Hubby says best pea soup yet!
This was really good. The only changes I made were: 2cans of chicken broth, instead of the bouillon cubes, therefore only 7 cups of water. I also added 1/2 lb or cubed ham and used Cajun sausage to add flavor. SDamare, Slidell,LA
Absolutely the best peas soup I have ever had!!!
I used 3/4 lb of italian sausage, and added a few peppers and red skin potatoes with skins. I like the extra fiber. :-) Thank you Dorothy for sharing this with us.
My husband and I like to make this recipe in the dead of winter! We like to beef it up a little with some Tabisco and a few other spices. We use chicken broth instead of chicken boullion cubes. That way there is not as much salt. Thank you for this recipe!
Yummy! I used turkey smoked sausage to cut the fat and this was really good. It freezes well too - I have it frozen in individual servings and have it for lunch often. Pretty easy to make too.
Amazing, even my 9 & 5 year old kids loved this. Husband went back for seconds too!!
I used 1lb of sweet Italian sausage squeezed from the casing and fried instead of the smoked sausage. I also used 8 cups of water instead of 10 because I only have a 4qut crock pot.
I ended up cooking it on High for about 5 hours and then low another 2 because I forgot about it :) it turned out great anyways and was nice and thick and creamy.
Went great with a box of Jiffy cornbread, an effortless meal to walk in the door to!
A definite keeper, THANKS!!
* Percent Daily Values are based on a 2,000 calorie diet.
Slow Cooker Split Pea Sausage Soup
Serving Size: 1/8 of a recipe
Servings Per Recipe: 8
Amount Per Serving
Calories: 412
Calories from Fat: 118
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See how to make hearty split pea soup from scratch.
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What are these called? Anyone ever use them? When were they available? They look like a Crack-n-up take-off. More views below.
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- 2
- people in the U.S. have this name View Chipp Miller on Whitepages
Meaning & Origins
The meaning of this name is unavailable
|80,650th in the U.S.
English and Scottish: occupational name for a miller. The standard modern vocabulary word represents the northern Middle English term, an agent derivative of mille 'mill', reinforced by Old Norse mylnari (see Milner). In southern, western, and central England Millward (literally, 'mill keeper') was the usual term. The American surname has absorbed many cognate surnames from other European languages, for example French Meunier, Dumoulin, Demoulins, and Moulin; German Mueller; Dutch Molenaar; Italian Molinaro; Spanish Molinero; Hungarian Molnár; Slavic Mlinar, etc.
|6th in the U.S.
Nicknames & variations
Chippy, Chip, Chippa, Chippewa, Chippie, Chippe, Chippah, Chippey, Chippi, Chippu
Mills, Milligan, Muller, Millard, Mallory, Millan, Millar, Milliken, Millsap, Millican


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Fanny Villagra's curator insight, February 17, 2014 4:28 AM
La curación de contenidos representa una evolución en la actividad de gestión de la información.
Laura Perez's curator insight, October 9, 2013 9:54 AM
I think this is a really important new role in our societies and we have to prepare the future adults in this kind of stuff.
Stephen Dale's curator insight, October 10, 2013 7:11 AM
I read somewhere else that 80% of people working in Social Media had been in their job for less than 2 years. Bear that in mind the next time you see a profile statiing "Black Belt", "Ninja" , "Guru" or "Expert" ! #socmed
Robin Martin's curator insight, October 10, 2013 9:30 PM
I saw this infographic just today, Susan! Gotta love it!
Jeff Domansky's curator insight, July 16, 2014 11:04 AM
Kevan Lee shares an invaluable list of WordPress blogging plugins to consider.
Jeff Domansky's curator insight, July 17, 2014 12:53 AM
These really are top WordPress plugins you should explore.
Jeff Domansky's curator insight, April 2, 2014 5:38 PM
Recent Twitter research may help you focus your business tweeting.
Beatriz Recio's curator insight, April 2, 2014 7:30 PM
¿Es Twitter efectivo como herramienta de branding para las marcas?
Abbey 's curator insight, April 7, 2014 4:45 PM
These are quite interesting statistics! I am one of those 52% wanting special offers/promotions and also the 33% that are hoping for freebies! I tend to only like a brand on Facebook or Twitter if I initially see advertising for a competition /discount. Lots of brands make statuses saying something along the lines of "like this status for the opportunity to win......" So if I see something like that I will automatically like it and then generally forget about the brand until something promoting the brand pops up on my news feed, where I will more often than not unlike the page! The fact that only 33% of a branders "Likers" are current customers is really interesting, to me that shows that the facebook page is creating brand awareness for the other 62% that are not initial customers. So all though majority of that 62% just wants something free our a special offer, they are still learning and become more aware of the brand which is a huge positive.
Bart van Maanen's curator insight, February 26, 2014 5:35 AM
LinkedIn komt met nieuwe, gevisualiseerde statistieken over bezoeken aan je profiel.
maxOz's comment, October 10, 2012 10:27 PM
Thanks Diane for your input. I agree with your sentiments, it's like a runaway train. Kids & Adults need ultimately to strive for "balance" probably one of the hardest skills to achieve. Cheers Michele
maxOz's comment, October 10, 2012 10:48 PM
Kirk, I try not to be, but I question the extent of my involvement.
Ellen Naylor's curator insight, August 15, 2014 12:09 PM
This is what I refer to as "technostress". It's easy to get sucked up in social media, and then wonder at the end of the why you got so little work done!
Steven Smith's curator insight, December 19, 2013 1:05 AM
Another apps for people to go crazy about. I guess, only makes the world easier to live in.
Jeff Domansky's curator insight, November 26, 2013 4:36 AM
Thoughtful post on the future of marketing.
Chris Perkowski's curator insight, February 18, 2014 5:27 PM
Facebook rose to dominance in 2008, and remains the top social network five years later. That's an unprecedented run, and despite constant criticisms about design changes and new features, it doesn't look like the social network will be dethroned any time soon.
But that doesn't mean we've reached the end of history for social networks. Several new ones have launched, such as Pinterest and Vine, and others have re-invented themselves. While Facebook is king, there's still room for niche alternatives, particularly those that emphasis images or video rather than an infinitely updating news feed.
Sometimes I feel as if there are too many social media platforms. There is now a website or an app for virtually anything you can think of. More social media platforms will still be developed in the future, however I feel that soon people will run out of innovative ideas to capture an audience that is already tied up in multiple platforms.


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Texas is losing agricultural land faster than any other state in the nation. The size of Texas disguises the urgency of the problem because it seems like we've got a lot of land. But if this trend continues, we could wake up one day and see that it has gone too far. What will we do then?Jim Bill Anderson
The woman who was the ranch's second owner had witnessed the impact of oil and gas development on the land of her childhood and she never forgot the changes that development brought. It was her wish that this ranch would stay the way it was – and we wanted the same thing. We enacted the conservation easement to keep it as open space forever.Mike Hughes


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The Long Night (Al Lail Altaweel)
Three long-term political prisoners are suddenly released - but why, and what awaits them? This elegant drama from controversial television miniseries director Hatem Mohamad Ali is a searing indictment of contemporary Syrian life and politics, yet universal in its intellectual, political, and moral fury.
Cast: Hatem Ali, Amal Arafah, Basel Khayat, Najah Safkouni, Khaled Taja
Produced by: Haitham Hakki
Directed by: Hatem Mohamad Ali
Written by: Haitham Hakki
Running time: 1hr 34min
|Theaters for Today
Pick a day
on this day.


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Nursing is a challenging and rewarding field, and we enjoy helping others get started in the profession.
Volunteer State Community College is fortunate to be able to offer students in the Gallatin area the opportunity to complete an AAS Degree in Nursing. The Nursing Program is actually a degree program of Tennessee State University that has been extended to the Gallatin campus.
The contract between Vol State and Tennessee State University specifies that the courses will be taught on the Vol State campus in Gallatin with clinical experience gained at hospitals as close to the Gallatin campus as possible. According to the contract, students are expected to take their general education courses at Volunteer State Community College (BIOLOGY, PSYCHOLOGY, ENGLISH, and everything else that is required that doesn't have NURSING in its title).
The grades for these courses will appear on a Vol State transcript. These Vol State grades will be transferred to TSU after the student has been accepted into the Nursing Program and substituted for the equivalent general education courses required of students enrolled in the program on the TSU main campus.
We REQUIRE you to attend one of our advisement sessions in which we will elaborate further on our program and respond to specific questions. If you bring transcripts of other college work, we can give you a good feel for whether or not you can receive transfer credit. If you are already an LPN, you will want to learn about our LPN challenge option. You will also be able to obtain all the forms for application that you will need and find out how to register for the pre-Nursing exam. These sessions will be held at the Vol State campus. Call (615) 230-3293 for dates and more information.
Steps in the Process
- Fill out an application to Volunteer State Community College.
- If needed, take the placement tests in Reading, Writing, and Algebra.
- Meet with Vol State advisor to schedule all required Remedial or Developmental refresher courses.
- Be certain that ALL Developmental courses (if required by the placement test) and CHEM 1030 (or one year of high school chemistry with a grade of C or better) are completed by the end of Fall term to be eligible for the class beginning the NEXT FALL TERM. The TSU Nursing Admissions Committee will decide during the Spring term who will be admitted for the upcoming Fall class.
- Attend one of the scheduled TSU Nursing Advising sessions. The schedule will be available from the TSU Office at VSCC (230-3293).
A student may request a Vol State application from the Office of Admissions (615) 230-3688 to be completed and returned. An application for admission to TSU may be requested from the Office of Admissions at Tennessee State University or from the TSU Office on the Vol State campus.
Many of the prospective Nursing students entering at Vol State have been out of school for some time. Students who are 21 or older will be REQUIRED to take a Tennessee Board of Regents' Placement test. This is the same placement test used at all Board of Regents' Community Colleges, Technical Institutes and Universities (TSU and Columbia State, for example). This test battery is part of the normal process of registering for college courses for all students who enter to pursue degrees. The test has parts on Reading, Writing, and Mathematics, including Algebra. Many students who enter college today need some help in reading, writing or math and, based on test results, must take refresher courses before they can enroll in college-level classes.
More Information
If you choose to complete all of the Vol State General Education courses required in the Pre-Nursing degree before taking any TSU Nursing courses, then the program will take longer than two years. Once enrolled in the first Pre-Nursing courses, program completion takes four semesters. Very few students choose to complete the Nursing Program in only two years.
There is tremendous interest in the Nursing profession and programs at Vol State and elsewhere are highly competitive. Planning ahead is absolutely essential. Please call the TSU Office at Vol State at (615) 230-3293, OR the Coordinator of the TSU Nursing Program at (615) 230-3282, OR a Secretary of the Division of Mathematics and Science at Vol State in Gallatin, TN at (615) 230-3261, OR a TSU Community College Recruiter at (615) 353-3577.


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After struggling with weight gain all her life and having multiple health problems as a result, Stacie decided to make a change. Learn how weight-loss surgery helped not only her health, but her outlook on life.
Note: All information is for educational purposes only. For specific medical advice, diagnoses, and treatment, consult your doctor.
© 1995-


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Spotlight Article:
The Curse of the TRC
Archaic utility cost effectiveness-testing is killing effective energy efficiency programs
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Hi All,
Due to upcoming Halloween holiday, we (Vladonai Software), in cooperation with DonationCoder, announce massive giveaway of AllMyNotes Organizer Deluxe Edition version 1.37 (worth $34 value), for free, full version.
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Hang tight!
Recipe by IRENED
"This pie is always well received whenever served - it is light and refreshing and very attractive, too. An all-time favorite! You may substitute the key lime juice with a mixture of 1/4 cup fresh lemon juice and 1/4 cup fresh lime juice."
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1 cup
graham cracker crumbs
3 tablespoons
white sugar
5 tablespoons
butter, melted
1/2 cup
key lime juice
1 (14 ounce) can
sweetened condensed milk
1 pinch
1 pinch
cream of tartar
1 cup
heavy whipping cream
lime, sliced
What an awesome recipe! This was the first key lime pie I've ever made, and it turned out fantastic! The second time I made it, I used fat-free sweetened condensed milk - big mistake - it was 'pasty.' I also reduced the amount of lime juice to make the pie a little firmer, and wished I hadn't - it didn't have enough lime flavor. The third time I made this, I reduced the butter by 1 Tbl., and used only 1/4 cup. I liked that much better. Don't be afraid to bake it the entire time, as that's what makes the filling firm and not runny. Note that this pie tastes much better the next day. Also, I liked increasing the crust ingredients per Ina Garten's recipe on FoodNetwork to 1 1/2 cups graham cracker crumbs, 1/4 cup sugar, and 6 Tbl. butter - makes a larger crust that is easier to work with, before and after baking. Note that you will need approximately 15 key limes + 1 regular lime OR 8-10 regular limes for the required amount of juice. Thanks so much for sharing your recipe!
I'm not sure if this is how key lime is supposed to taste, but it really wasn't what I was looking for. The whipped egg whites gave it an airy, foamy texture. If you're into that kind of thing, it definitely is a good recipe. I was looking for something a bit more smooth and creamy. I wouldn't make this pie again.
251 Ratings
If you are looking for something light, fluffy and delicious- this is it! I used 1 1/2 cans condensed milk; 2/3 key lime juice; 3 jumbo egg yolks (no whole eggs); and folded in 2 whipped egg whites. I added 1 ounce of lime jello for color and extra flavor. Wonderful! (I did not freeze it.)
I only got a tiny taste of this pie which i made for my father in law, who is a key lime pie lover. He said it was the best keylime pie he's ever had. It's hard to beat my mother in laws cooking, but she called ME and asked for the recipe! I'll make this particular recipe for him every year. It's quick, simple and got high marks with the family.
I had no trouble w/this turning out smooth and silky. I did make the crust. I had a 9.5 inch pie dish so I just crushed up one entire package of graham crackers and added an extra Tbl spoon of Butter and little extra sugar to the crust mix and it turned out fine! I didnt bother letting it cool in the freezer as long as the recipe suggested. I pulled the finished pie out of the oven, let it cool for about 30min, popped it in the freezer for 1hr, then I topped it w/cool whip and placed it in the fridge and the texture/temp was just right! I will make this again for sure!
After hearing other people saying that the filling was not enough for a pie, i decided to double the recipe and it was delicious. I put lime peel in it also. If you don't want to waste the egg whites, make a meringue otherwise, it was good without the whipped cream.
This is the second time this week I've made this pie..To die for! I just wasn't sure if it was suppose to be served frozen, or froze then refrigerated (thats how I ended up doing it) Yum
Very nice recipe. I thought the taste was just right. I wish it was a bit thicker though - maybe I will add more filling next time. I made it in a springform pan and that helped with getting the pie out. I always detroy pies when they are in the traditional pan. I enjoyed it frozen and just chilled in the fridge as well.
* Percent Daily Values are based on a 2,000 calorie diet.
Key Lime Pie I
Serving Size: 1/8 of a recipe
Servings Per Recipe: 8
Amount Per Serving
** Calories: 420
** Calories from Fat: 229
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Serve this colorful crostini at your next party and the plates will be empty before you know it!
Hors D'Oeuvres &
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May 2007
Product Reviews / Main Nibbles / Hors D'Oeuvres & Cocktail Snacks
This asparagus recipe, courtesy of Sargento Cheese Artisan Blends, is perfect to serve at parties, as hors d'oeuvres or a first course. The crostinis are easy to eat and look great on a platter. Not to mention, they are absolutely delicious, and everyone will want the recipe. If you want a vegetarian dish, substitute vegetarian bacon for the pancetta.
The crunchy crostini and the ricotta and parmesan crisps can be made two days ahead of time (refrigerate the ricotta mixture and store the crisps and crostinis in airtight containers).
Asparagus are available year-round, although spring is the best season for fresh asparagus. April through late June is prime asparagus season; however, now that much of our asparagus is being grown in South America instead of the Pacific Northwest, there may be a seasonal shift. Read more about the types of asparagus in our recipe for Linguine With Asparagus And Parma Ham.
Ricotta Mixture
Parmesan Crisp
In a medium sauté pan, place the pancetta over medium heat. Cook until pancetta is crisp and brown around the edges, about 6 minutes.
Spread about 1 teaspoon of Ricotta-Parmesan mixture onto each crostini. Lightly press 5 or 6 slices of asparagus and 3 or 4 pieces of pancetta into the cheese mixture.
Recipe and photo © Sargento Cheese. All other material © copyright 2005-
2015 Lifestyle Direct, Inc. All rights reserved. Images are the copyright of their respective owners.


text:
Counter screen for identification of compounds that inhibit regulator of G-protein signaling 4 (RGS4): Non-induced cells with the primary screen assay
Assay Implementation: Zhihong Lin Ph.D., Joseph Babcock, Xiaofang Huang M.S., Shunyou Long M.S., David Meyers Ph.D., Owen McManus Ph.D., Meng Wu Ph.D. ..more
BioActive Compounds: 386
Related Experiments
Data Source: Johns Hopkins Ion Channel Center (JHICC_RGS_Inh_Counter1)
BioAssay Type: Primary, Primary Screening, Single Concentration Activity Observed, Duplicate
Source (MLPCN Center Name): Johns Hopkins Ion Channel Center (JHICC)
Center Affiliation: Johns Hopkins University, School of Medicine
Screening Center PI: Min Li, Ph.D.
Assay Provider: Richard Neubig, M.D., Ph.D. University of Michigan
Network: Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Proposal Number: 1 R03 MH087441-01A1
Grant Proposal PI: Richard Neubig, M.D., Ph.D.
Assay Implementation: Zhihong Lin Ph.D., Joseph Babcock, Xiaofang Huang M.S., Shunyou Long M.S., David Meyers Ph.D., Owen McManus Ph.D., Meng Wu Ph.D.
Name: Counter screen for identification of compounds that inhibit regulator of G-protein signaling 4 (RGS4): Non-induced cells with the primary screen assay.
Signal transduction pathways mediated by G protein-coupled receptors (GPCRs) are major therapeutic targets with many known pharmacologic modulators. The regulators of G protein signaling (RGS proteins) are a key family of modulators and scaffolds for GPCRs which have been identified by many authors as appealing drug targets [1-5]. RGS proteins bind to activated G proteins and strongly inhibit their signals by catalyzing the hydrolysis of bound GTP [6-7]. Consistent with this mode of action, genetic deletion of RGS greatly enhances receptor signaling [8-10]. Furthermore, RGS proteins display isoform-specific tissue distribution [11-14] and their expression is altered in pathophysiological states including Parkinson's disease [15], neuropathic pain [16-17], schizophrenia, hypertension, and congestive heart failure [18]. An activator of RGS action would be expected to suppress the function of GPCR agonists such as vasoactivators, inflammatory mediators, endogenous neurotransmitters, or catecholamines such as L-DOPA. It has recently been established that loss of function of RGS2 causes hypertensive symptoms in mice [19] and may contribute to vasoactive disorders in humans as well [20-22]. Also, reduced expression of RGS4 has been correlated with schizophrenia [23-24]. Consequently, compounds that could either enhance the function of RGS proteins or could increase their expression might be of therapeutic benefit. The identification of selective modulators of RGS proteins would also greatly facilitate experimental studies of this sizeable protein family (with 20-30 members) by permitting rapid and specific chemical potentiation. Such RGS modulators would be particularly useful to determine which RGS proteins modulate particular receptor signaling pathways.
The goal of this project is to identify inhibitors of the regulator of G protein signaling 4 (RGS4), using a receptor-stimulated cell-based Ca++ assay.
Assay overview:
To counter screen the hit compounds that inhibit the RGS4 protein from the primary screen, the Non-induced same HEK293 cell line which stably expresses M3R and inducibly expresses RGS4 is employed. RGS4 function is monitored by calcium flux with a commercially available Fluo4-AM dye. Compounds that show increase in the Fluo4 fluorescence in non-induced RGS4 expressed cells are considered non-specific hits.
[1]. Zhong, H., and Neubig, R.R. 2001. Regulator of G protein signaling proteins: novel multifunctional drug targets. J Pharmacol Exp Ther 297:837-845. PMID: 11356902.
[2]. Neubig, R.R., and Siderovski, D.P. 2002. Regulators of G-protein signalling as new central nervous system drug targets. Nat Rev Drug Discov 1:187-197. PMID: 12120503.
[3]. Druey, K.M. 2003. Regulators of G protein signalling: potential targets for treatment of allergic inflammatory diseases such as asthma. Expert Opin Ther Targets 7:475-484. PMID: 12885267.
[4]. Cho, H., Harrison, K., and Kehrl, J.H. 2004. Regulators of G protein signaling: potential drug targets for controlling cardiovascular and immune function. Curr Drug Targets Immune Endocr Metabol Disord 4:107-118. PMID: 15180451.
[5]. Riddle, E.L., Schwartzman, R.A., Bond, M., and Insel, P.A. 2005. Multi-tasking RGS proteins in the heart: the next therapeutic target? Circ Res 96:401-411. PMID: 15746448.
[6]. Druey, K.M., Blumer, K.J., Kang, V.H., and Kehrl, J.H. 1996. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature 379:742-746. PMID: 8602223.
[7]. Yan, Y., Chi, P.P., and Bourne, H.R. 1997. RGS4 inhibits Gq-mediated activation of mitogen-activated protein kinase and phosphoinositide synthesis. J Biol Chem 272:11924-11927. PMID: 9115254.
[8]. Jeong, S.W., and Ikeda, S.R. 2000. Endogenous regulator of G-protein signaling proteins modify N-type calcium channel modulation in rat sympathetic neurons. J Neurosci 20:4489-4496. PMID: 10844018.
[9]. Clark, M.J., Neubig, R.R., and Traynor, J.R. 2004. Endogenous regulator of G protein signaling proteins suppress Galphao-dependent, mu-opioid agonist-mediated adenylyl cyclase supersensitization. J Pharmacol Exp Ther 310:215-222. PMID: 15014136.
[10]. Fu, Y., Zhong, H., Nanamori, M., Mortensen, R.M., Huang, X., Lan, K., and Neubig, R.R. 2004. RGS- insensitive G-protein mutations to study the role of endogenous RGS proteins. Methods Enzymol 389:229-243. PMID: 15313569.
[11]. Gold, S.J., Ni, Y.G., Dohlman, H.G., and Nestler, E.J. 1997. Regulators of G-protein signaling (RGS) proteins: region-specific expression of nine subtypes in rat brain. J Neurosci 17:8024-8037. PMID: 9315921.
[12]. Nomoto, S., Adachi, K., Yang, L.X., Hirata, Y., Muraguchi, S., and Kiuchi, K. 1997. Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization. Biochem Biophys Res Commun 241:281-287. PMID: 9425263.
[13]. Kurrasch, D.M., Huang, J., Wilkie, T.M., and Repa, J.J. 2004. Quantitative real-time polymerase chain reaction measurement of regulators of G-protein signaling mRNA levels in mouse tissues. Methods Enzymol 389:3-15. PMID: 15313556.
[14]. Doupnik, C.A., Xu, T., and Shinaman, J.M. 2001. Profile of RGS expression in single rat atrial myocytes. Biochim Biophys Acta 1522:97-107. PMID: 11750060.
[15]. Tekumalla, P.K., Calon, F., Rahman, Z., Birdi, S., Rajput, A.H., Hornykiewicz, O., Di Paolo, T., Bedard, P.J., and Nestler, E.J. 2001. Elevated levels of DeltaFosB and RGS9 in striatum in Parkinson's disease. Biol Psychiatry 50:813-816. PMID: 11720701.
[16]. Garnier, M., Zaratin, P.F., Ficalora, G., Valente, M., Fontanella, L., Rhee, M.H., Blumer, K.J., and Scheideler, M.A. 2003. Up-regulation of regulator of G protein signaling 4 expression in a model of neuropathic pain and insensitivity to morphine. J Pharmacol Exp Ther 304:1299-1306. PMID: 12604710.
[17]. Xie, G.X., and Palmer, P.P. 2005. RGS proteins: new players in the field of opioid signaling and tolerance mechanisms. Anesth Analg 100:1034-1042. PMID: 15781518.
[18]. Mittmann C, Chung CH, Hoppner G, Michalek C, Nose M, Schuler C, Schuh A, Eschenhagen T, Weil J, Pieske B, Hirt S, Wieland T. 2002. Expression of ten RGS proteins in human myocardium: functional characterization of an upregulation of RGS4 in heart failure. Cardiovasc Res 55:778-786. PMID: 12176127.
[19]. Heximer SP, Knutsen RH, Sun X, Kaltenbronn KM, Rhee MH, Peng N, Oliveira-dos-Santos A, Penninger JM, Muslin AJ, Steinberg TH, Wyss JM, Mecham RP, Blumer KJ. 2003. Hypertension and prolonged vasoconstrictor signaling in RGS2-deficient mice. J Clin Invest 111:445-452. PMID: 12697745.
[20]. Bodenstein, J., Sunahara, R.K., and Neubig, R.R. 2007. N-terminal residues control proteasomal degradation of RGS2, RGS4, and RGS5 in human embryonic kidney 293 cells. Mol Pharmacol 71:1040-1050. PMID: 17220356.
[21]. Gu, S., Tirgari, S., and Heximer, S.P. 2008. The RGS2 gene product from a candidate hypertension allele shows decreased plasma membrane association and inhibition of Gq. Mol Pharmacol 73:1037-1043. PMID: 18230714.
[22]. Yang J, Kamide K, Kokubo Y, Takiuchi S, Tanaka C, Banno M, Miwa Y, Yoshii M, Horio T, Okayama A, Tomoike H, Kawano Y, Miyata T. 2005. Genetic variations of regulator of G-protein signaling 2 in hypertensive patients and in the general population. J Hypertens 23:1497-1505. PMID: 16003176.
[23]. Chowdari KV, Mirnics K, Semwal P, Wood J, Lawrence E, Bhatia T, Deshpande SN, B K T, Ferrell RE, Middleton FA, Devlin B, Levitt P, Lewis DA, Nimgaonkar VL. 2002. Association and linkage analyses of RGS4 polymorphisms in schizophrenia. Hum Mol Genet 11:1373-1380. PMID: 12023979.
[24]. Mirnics, K., Middleton, F.A., Stanwood, G.D., Lewis, D.A., and Levitt, P. 2001. Disease-specific changes in regulator of G-protein signaling 4 (RGS4) expression in schizophrenia. Mol Psychiatry 6:293-301. PMID: 11326297.
[25]. Miret, Juan J., et al., Multiplexed G-Protein-Coupled Receptor Ca2+ Flux Assays for High-Throughput Screening. J Biomol Screen 10: 780-787 (2005) PMID: 16234348.
[26]. Zhang, J.-H., T.D.Y. Chung, and K.R. Oldenburg, A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen, 4(2), 67-73 (1999) PMID: 10838414.
[27]. Malo, N., et al., Statistical practice in high-throughput screening data analysis. Nat Biotech, 24(2), 167-175 (2006). PMID: 16465162.
RGS4, M3 receptor, 384, primary, antagonist, inhibitors, allosteric, FDSS, calcium, fluorescence, Kinetic, Fluo-4-AM, JHICC, Johns Hopkins, MLSMR, Molecular Libraries Probe Production Centers Network, MLPCN.
Protocol for RGS4 Primary Screen:
1. Cell culture: Cells (HEK293-FlpIn-TREx/M3R/RGS4) are routinely cultured in DMEM (high glucose, w/ glutamine), 10% FBS, 1%Pen/Strep, 15 ug/ml Blasticidin, 400 ug/ml G418, 200 ug/ml Hygromycin.
2. Cell plating: Add 50 ul/well of 200,000 cells/ml re-suspended in DMEM/high glucose medium with 10% FBS, 1%Pen/Strep, WITHOUT 10 ng/ml Doxycyclin (DOX) to induce RGS4 expression.
3. Incubate overnight at 37 degrees C and 5% CO2.
4. Remove medium and add 20 ul /well of 2 uM Fluo4-AM solution to cells.
5. Incubate 30 minutes at 37 degrees C in incubator.
6. Prepare 6x compound plates and control plates on Cybi-Well system: test compounds are prepared using assay buffer (HBSS-HEPES pH 7.4).
7. Remove Fluo4-AM dye solution and add 20 ul /well of assay buffer to cells.
8. Incubate 30 minutes at room temperature (RT).
9. Add 6x compounds in cell plates and incubate 20 minutes at RT.
9. Load cell plates on Hamamatsu FDSS 6000 kinetic imaging plate reader
10. Measure fluorescence for 10 seconds at 1Hz to establish baseline
11. Add 4 ul of 7x EC20 (carbachol) into the cell plates and record fluorescence for 100 seconds.
12. Calculate ratio readout as F(max-min)/F0 and integrated ratio readout.
13. Calculate the average and standard deviation for negative and positive controls in each plate, as well as Z and Z prime factors [26]
14. Calculate the percentage of tested compounds with the following formula:
Percentage (%)=100* (Ratio(cmpd)- AvgRatio(Buffer))/(AvgRatio(Carbachol)-AvgRatio(Buffer)); Percentage(%): percentage change of compound readout over those of negative controls (Buffer), Ratio(cmpd): Integrated ratio of the test compound. AvgRatio(Buffer): Integrated Ratio average of the negative controls with Buffer, Ratio(Carbachol): Integrated Ratio of Carbachol.
15. Outcome assignment: If the compound (the average of the duplicates of the Percentage (%, avPercent) as readout) causes more than those of negative controls (Buffer) plus 5SD of negative controls (Buffer), the compound is considered to be active (Value=2). Otherwise, it is designated as inactive (Value=1).
16. Score assignment: An active test compound is assigned a score between 5 and 100 by calculation of Int((Lg(abs([avPercent]))-1.42)*145.6). The inactive test compounds are assigned a score of 0.
List of reagents
1. HEK293-FlpIn-TREx/M3R/RGS4 cell lines (provided by assay provider)
2. PBS: pH7.4 (Invitrogen Catalog number 10010049)
3. Medium: DMEM (Sigma, Catalog number D5796)
4. Fetal Bovine Serum (Gemini, Catalog number 100-106)
5. Hygromycin (Mediatech, Catalog number 30-240-CR)
6. 100x Penicillin-Streptomycin (Mediatech, Catalog number 30-001-CI)
7. Cell/stripper (Mediatech, Catalog number 25-056-Cl)
8. G418: (Invitrogen, Catalog number 11811-031)
9. Blasticidin (Sigma, Catalog number R21001)
10. Doxycycline hyclate (Sigma, Catalog number D9891)
11. HEPES (Sigma, Catalog number H4034)
12. Fluo-4 (Invitrogen, Catalog number F14202)
13. Pluronic F-127*20% in DMSO (Invitrogen, Catalog number P-3000MP)
14. Atropine (Sigma, Catalog number A0132)
15. Carbachol (Sigma, Catalog number C4382)
16. Triple-layer flask (VWR, Catalog number 62407-082)
17. BD Biocoat 384-well plates (BD, Catalog number (35)4663 and Lot number 7346273)
Possible artifacts of this assay can include, but are not limited to: non-intended chemicals or dust in or on wells of the microtiter plate, compounds that non-specifically modulate the cell host or the targeted activity, and compounds that quench or emit light or fluorescence within the well. All test compound concentrations reported are nominal; the specific concentration for a particular test compound may vary based upon the actual sample provided by the MLSMR.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Cell Type: HEK293
Result Definitions
** Test Concentration.
Additional Information
Grant Number: 1 R03 MH087441-01A1
Data Table (Concise)


text:
I am looking for all kinds of research concerning option trading strategies. With that I mean papers that publish results on different option trading strategies properly backtested with real-world data.
Take the 2-minute tour ×
Quantitative Finance Stack Exchange is a question and answer site for finance professionals and academics. It's 100% free, no registration required.
I did some digging and found the following papers - most of them offering quite a distinct perspective compared to classical option pricing theory!
The following is my favorite: You could do some backtests on your own with freely available data (using the VXO as volatility information) and with any spreadsheet - easy and elegant:
Option Traders Use (very) Sophisticated Heuristics, Never the Black–Scholes–Merton Formula
Stock Price Clustering on Option Expiration Dates
Since I, too, have been very interested in this question, I will share some of my findings in the dual hope of encouraging comments on the papers and eliciting more activity on this question.


text:
Additional Information
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I'm currently trying to figure this out...it seems a little complicated and I haven't found any resources that explain it clearly.
Take the 2-minute tour ×
Web Applications Stack Exchange is a question and answer site for power users of web applications. It's 100% free, no registration required.
There are 2 ways either via putting double brackets around it:
and there will be a new one called "Test"
OR via using camelcase, see: http://en.wikipedia.org/wiki/CamelCase
Just follow the default tutorial: http://www.blogjones.com/TiddlyWikiTutorial.html
In step 4 it will open "HowToMakeATiddler"


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I don't think so. But we will see for sure soon enough. I can't wait to see where we're at when I get home tonight.Originally Posted by outtawack311
I don't think so. But we will see for sure soon enough. I can't wait to see where we're at when I get home tonight.Originally Posted by outtawack311
Jimmy Johnson might say, "Atta boy Muck go gettum Muck go gettum Muck go gettum Muck gettum gettum Yeah!!!!. That's the way, son!! That's the way!! <clapclapclapclapclapclapclapclap>"Originally Posted by Muck
Bill Parcells might say, "You took a false step there, Muck. Now I don't want to see that sh*t again. Got it? I don't wanna see that sh*t again".
Originally Posted by Muck
''There were a page not found in browsers early, but nothing we can't clean up,'' Wannstedt said.
Wansteadt was quoted as saying "From a site reliability standpoint, we're not quite where we want to be. We feel that we're just a piece or two away from being there. We're going to get this thing fixed."
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Nicktator Saban
Apparently the boards went down today... I received this from my host:
At approximately 3:15PM (CST) 05/03/2004 our Hurricane Electric facilities suffered a power outage. This cause all servers to loose power and our onsite engineers are working rack to rack to bring them up.
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A Community-Developed Dictionary of Software Weakness Types
Version 2.8 posted July 31, 2014. There were 58 new entries. There were major changes to 638 entries in support of Software Fault Patterns and the State-of-the-Art Resources (SOAR) report, primarily affecting names, relationships, detection methods, taxonomy mappings, and demonstrative examples. There was a minor schema update. Read the
release notes.
More Information
CWE is co-sponsored by the office of Cybersecurity and Communications at the U.S. Department of Homeland Security.
This Web site is sponsored and managed by The MITRE Corporation to enable stakeholder collaboration. Copyright © 2006-2015, The MITRE Corporation. CWE, CWSS, CWRAF, and the CWE logo are trademarks of The MITRE Corporation.
Contact email@example.com for more information.


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text:
I need a reference for conditions on a closed subspace of a Banach space to have the homotopy type of an ANR.
Take the 2-minute tour ×
MathOverflow is a question and answer site for professional mathematicians. It's 100% free, no registration required.
You could try Karol Borsuk's Theory of Retracts. There is extensive discussion of ANRs. Further related conditions can be found in shape theory (work in the 1970s by Edwards and Geoghegan) but that requires a knowledge of shape theoretic ideas. (That is why I asked what sort of conditions you were looking for as this is taking you away from the central area of Banach spaces).
(By subspace you mean topological subspace, right?).
You may try papers by Henryk Toruńczyk (and his students).


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by Ekornes
The Stressless E300 Loveseat is one of Ekornes' more modern designs as far as style cues go. But don't be fooled -- it has the brains and brawn to match its beauty, while all the time providing the absolute comfort that Stressless is so well-known for. Now with fully padded armrests, comfort is taken to another level no matter how you decided to sit.
Featuring the Stressless ErgoAdapt System, the seat tilts down automatically the moment you sit down, so your sitting angle is optimized to you. And, if you want to lie down, the seat elevates to the completely flat position. No matter what your choice, the outcome is the same -- ultimate comfort.
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Materials & MeasurementsDimensions:
Overall: 28.75" h x 72.5" w x 38.25" d
Seat height: 18.1"
Seat depth: 22.4"
Materials & Fabrics:
To get a closer look at the upholstery options and wood finishes for the Stressless E300 Loveseat, visit our Ekornes Stressless Materials Library.
Your FurnitureGenius Recommendations
About The ManufacturerEkornes® Stressless®
Comfort is the primary goal of the entire Stressless line. Since 1971, when they first introduced themselves to the world, Ekornes has been interested in bringing the world's most comfortable seating experience to a wider and wider audience.
Ekornes is a Norwegian company, and they made their name and reputation on the Stressless line of home furnishings. While the Stressless Recliner is undoubtedly their most significant product in a historical sense, the Ekornes Stressless Sofa concedes no ground when it comes to the three most important aspects of home furnishing design; comfort, support, and style. In this sense the sofas are not redundant products, but serious and inspired variations and continuations on a theme; total comfort, total relaxation, total balance and fit. The principles of the Ekornes lines were laid out in detail with the first recliners, but only with the advent of the Ekornes Stressless Sofa has the fullness of their vision come into clear focus.
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Shipping & Delivery
The Ekornes Stressless E300 Loveseat ships via standard ground shipping within the contiguous United States.
Product Q&A
Q: Are there any other coordinating pieces with the Stressless E300 Loveseat?
A: Absolutely. The Stressless E300 collection also features a matching Chair and Sofa.
Frame: warranted for 10 years
Foam casings: warranted for 5 years
Wood base: warranted for 5 years
Leather/fabric: warranted for 1 year
Ekornes guarantees the internal mechanism of our recliners for a period of 10 years from the date of purchase of the product. The 10 year guarantee covers the Plusâ"¢-system and the internal metal frame, including the metal springs and gliding system. It does not cover damage to leather or woodwork or any faults or damage caused by the use of the product other than in accordance with the manufacturer's recommendations, as well as any faults or damage arising from any repairs made by any persons not authorized by the manufacturer.


text:
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By: David Spero
Dear Diabetes Health,
I am a 29-year-old man who was diagnosed two years ago with type 1 diabetes. I had a pretty good sex life before the diagnosis, although I almost always had a couple of drinks before trying anything. They helped me relax.
Now, if I have a couple of drinks, I can't perform. Drinking seems to just put me to sleep. But without it, I'm tense, which isn't good either. Is this alcohol reaction part of diabetes? What can I do about it?
Frustrated Type 1
Dear FT1,
You have raised two important issues: alcohol's effect on diabetes and alcohol's effect on sex. The two effects are related. Let's start with sex.
Alcohol's Effect on Sex
You feel that a couple of drinks make you more relaxed about sex. We're sure that's true. Alcohol is well known for reducing inhibitions, making it easier to function in social situations. The inner voice warning you that "This is scary" is turned down. That's why alcohol is often used by men and women to get ready for sex, and even for flirting or dating.
A couple of drinks may also make you feel more attractive and confident. This feeling obviously helps in approaching others or being in close contact. It may even make you more attractive to some potential partners. Confidence is a good thing.
Alcohol can also have the effect of making other people seem more attractive to you. This phenomenon (supported by science) is sometimes called "beer goggles," defined by the Urban Dictionary as "a condition afflicting inebriated individuals, allowing mutual attraction between those who would ordinarily not be attracted."
When people get much beyond two drinks, however, all these sex-enhancing effects become overwhelmed by the depressant and sedative effects of alcohol. You say that you "can't perform" after drinking. We don't like to think of sex as "performance," but we understand your concern. After only two years of diabetes, with reasonably good control, this is probably not a problem of physical damage. It's probably the booze. Erection problems with alcohol are so common they have their own name: "Brewer's Droop."
You may not have noticed such problems before, but you're on insulin now, and that changes things. Here's how diabetes gets into the alcohol/sex equation.
Alcohol's Effect on Diabetes
The most common effect of alcohol on blood glucose is that it can make your sugars go low. According to December 2001 Nutrition Research Letter, "it has been estimated that as many as one-fifth of severe hypoglycemic episodes are directly related to alcohol."
Exactly how alcohol brings down sugars is unknown. One theory is that alcohol lowers cortisol levels. Cortisol is a stress hormone that keeps sugars up, so lowering it could cause glucose to drop, too.
Another theory concerns the liver. According to Amy Campbell, RD, author of Staying Healthy with Diabetes, "In most cases, your liver is able to release glucose when blood glucose levels start to drop, in an effort to prevent hypoglycemia. But with alcohol on board, this may not happen."
Campbell says the body treats alcohol as a poison, not a food. It sends alcohol to the liver as fast as it can, and the liver starts detoxifying it. Detoxification is a complex process that takes up most of the liver's attention. So the liver may temporarily forget its other functions, like keeping your blood glucose up.
You may not get the sweating, shaking, word-slurring kind of hypoglycemia when you drink, but even a small drop in glucose can affect erections and sexuality. In response to an earlier column about ED, several readers commented that their erection problems usually developed when their glucose levels went below about 90 mg/dL. When they got their levels up between 110 and 120, erectile function returned.
For people with type 2, alcohol can lower your glucose in another way, by stimulating insulin production. This may be a good thing after meals, but it's probably not when you're trying to be sexual. Of course, high blood sugars can interfere with sex, too. But alcoholic drinks usually don't raise sugars much.
Sex Without Alcohol
FT1, our wish for you is that you learn to relax and feel confident without alcohol. That would be the best way to deal with your situation. You could talk with your doctor about a prescription or over-the-counter medication, but you probably don't need drugs-and don't forget that alcohol itself is a drug.
Try focusing on your breathing when you're in a social situation or a sexual situation. Sex educator Annie Sprinkle says, "The single most important key to sex that I've yet discovered is conscious rhythmic breathing; the more you breathe, the more you feel and the more you come alive."
In social situations, remember to take regular deep breaths (not so deep as to have everyone staring at you, just comfortably deep). Try breathing in through your nose, into your abdomen, and out through your mouth. Or practice the relaxed breathing exercises here. Even in bed, remember to breathe. Your partner and you will both enjoy the experience more if you are relaxed and breathing deeply.
There's a kind of breathing meditation called Tantra, which is often associated with sex. You can see some tantric breathing exercises here.
The Bottom Line
The American Diabetes Association and other authorities say that moderate drinking is OK for people with diabetes. "Moderate" means two drinks a day for men and one for women. A drink is usually defined as about five ounces of wine, 1½ ounces of hard liquor, or 12 ounces of beer. Studies have shown definite health benefits associated with moderate drinking. With greater amounts, however, a lot of bad things can happen-from liver disease to auto accidents.
For sex with diabetes, though, many people will do best to avoid alcohol entirely. Try some breathing and relaxation exercises and see what happens.


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Gucci hoochi: 2012 Fiat 500 Cabrio
As small cars start to gain acceptance from American drivers, Scion has entered the market with a new one that ranks high on intelligence – the iQ. As the name implies, this little car has some smart features and is much more than simply small and economical.
BEVERLY HILLS (AP) - Stand on the Beverly Hills Hotel's red carpet, leading into its chandeliered lobby, and you can't help but visualize a century's worth of celebrities, royalty, politicians, musicians and actors who have stayed there, from Marilyn Monroe and Elizabeth Taylor to Madonna, Reese Witherspoon and Katy Perry.
Drivers count on their vehicles to get them to their destinations safely, but how well a vehicle runs depends on how much effort motorists put into protecting their investment in their cars.
The 2012 Passat is Volkswagen's first German-American offspring that satisfies for its European sensitivity and U.S.-spec comfort and compliance. The new model was re-engineered to meet the demands and expectations of this country, and it is the first model built at the new factory in Chattanooga, Tenn.
DETROIT (AP) - Toyota is back. It's putting a year of earthquake-related shortages behind it and grabbing sales from stumbling General Motors and Ford.
There's an old adage that says, "Put your best foot forward", and that's exactly what Chevrolet is doing with the introduction of the new Malibu. With gasoline pumps becoming all too familiar with the $4 a gallon gasoline, the Chevy planners are sending out the fuel-sipping Malibu Eco first as an introduction to the all-new Malibu lineup. After all, who can argue with a gasoline powered midsize sedan getting fuel economy of 25 mpg city and 37 mpg highway.
The 2012 Laramie 2500 and 3500 models are built for work or play.
The Mini Cooper Coupe is a glove. Put your hand in and go out for some fun. It is just that kind of car and it's how it fits the driver. But this is not the typical point-and-shoot two-seater; this coupe actually has decent trunk capacity by a liftback design.
It's not hard to see why Lake Arrowhead has always been popular with the movie stars – it's close to Los Angeles, yet light years away in attitude. The people with money are here, all right, but there is also an element of small-town America, complete with families and, yes, even family values.
The 2012 Kia Rio LX is a sporty five-door hatchback now available at Kia Country, 1515 N. Main St., Manteca.
BERLIN (AP) - Volkswagen AG's Audi unit said Wednesday it will buy Italian high-performance motorcycle maker Ducati Motor Holding SpA from Investindustrial Group. The move gives VW another glossy brand to go with its Lamborghini, Bugatti and Bentley luxury auto makers.
NEW YORK (AP) - Americans were laughing at Hyundai's cars when John Krafcik joined the company eight years ago.
WASHINGTON (AP) - New cars and light trucks would have to have override systems to prevent unintended acceleration when the gas pedal and brake are applied at the same time or accelerator control systems disconnect, under new federal regulations proposed Thursday.
DETROIT (AP) - Americans are buying record numbers of hybrid and electric cars as gas prices climb and new models arrive in showrooms, giving the vehicles their greatest share yet of the U.S. auto market.
Page 23 of 26
FLAGSTAFF, Ariz. (AP) - A geological gem awaits hikers in the wilderness along the Arizona-Utah border. Swirls of searing reds, oranges and yellow fold into a ...
The habitat of the big truck-based SUV has been decimated in most regions of the U.S., not just by a downsized economy but also ...
Bigger is better this year in Chevrolet's largest sport utility vehicle, the Suburban.
Last year's Califor-nia Small Business of the Year, Phil Water-ford's Manteca Ford was responsible for $10,004 of the $90,340 raised by ...
Do you remember "Matthew Hernandez"? He is the 16 year old stu-dent/athlete who found a wallet during the win-ter holidays several years ago with ...
Most people know Ste-phen Curry as the "leader" of the Golden State Warriors. He has won the NBA Most Valuable Player award for his championship ...
Page 1 of 1
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text:
ChuckE2009 just posted a video called: StickWeld 250 Torture Test + Full Review - https://www.youtube.com/watch?v=g-ssv_K8ZL4.
If you start viewing at 13 minutes and then the rest of the video you'll see what running bead practice looks like.
I'm a noob at this too and I thought I was doing good at practice time but I'm still working my way through a 10lb box of 1/8 7018. I've been burning other electrodes and wire too so I might have burned through this box by now if not for that. Not sure I could do a whole box in one sitting. :-) Going to have to look at adding machines with higher duty cycles to my collection I guess.
Results 1 to 4 of 4
01-20-2013, 11:37 AM #1Member
- Join Date
- Jul 2012
- Posts
- 87
What Running Bead Practice Looks Like==============
Miller 211 MVP
Hobart StickMate LX 235
Lincoln AC 225
CutMaster 42
01-20-2013, 11:48 AM #2Senior Member
- Join Date
- Nov 2012
- Location
- Northern Arizona
- Posts
- 571
I'll go watch the video to see what your talkinga bout in a little bit..
When I first got my cst280 I hooked it up to 1ph 230v power. That meant my max output was 200amps @50%dc and 150 amps @100% duty cycle. I burned through 5# of 6011 and 7018. Didn't even the get fan to turn on! The other day I was DC TIG welding with it and was about 80% through a project with about 9 feet of TIGing. The fan turned on for about 10 seconds! I was glad to hear it. That meant that it actually works. Up until this point I wasn't sure! I was thinking about selling it to help towards an ac/dc tig machine but stuff in the 200amp inverter class just doesn't have the duty cycle that this thing does. So for now I'm keeping it for straight stick welding.MillerMatic 251
Maxstar 150 STH
Cutmaster 42
Victor Journeyman OA
A rockcrawler, er money pit, in progress...
I'll stick with Miller, but thanks for the review ChuckE2009.at home:
2012 325 Trailblazer EFI with Excel power
2007 302 Trailblazer with the Robin SOLD
2008 Suitcase 12RC
Spoolmatic 30A
2009 Dynasty 200DX
2008 Thermal Dynamics Cutmaster 52
Sold:MM 251
Sold:CST 280
at work:
Invision 350MP
Dynasty 350
Millermatic 350P
Retired:Shopmaster 300 with a HF-251
01-21-2013, 10:06 AM #4Nothing welded, Nothing gained
Miller Dynasty700DX
3 ea. Miller Dynasty350DX
Miller Dynasty200DX
ThermalArc 400 GTSW
MillerMatic200 with spoolgun
Lincoln SP-170T
Linde UCC305 (sold 2011)
Hypertherm 1250
Hypertherm 800
PlasmaCam CNC cutter
Fadal Toolroom CNC Mill
SiberHegner CNC Mill
2 ea. Bridgeport
LeBlond 15" Lathe
Haberle 18" Cold Saw
Doringer 14" Cold Saw
6 foot x 12 foot Mojave granite


text:
Kosher Salads & Salad Dressings
|Chopped Egg Salad with Watercress
Adapted from Silvermark
This simple-to-prepare egg salad makes an elegant addition to a luncheon buffet. If you omit the peas, it is delicious on soft sandwich rolls. This salad is wonderful in the summer, served on a lettuce leaf, or in the winter with a bowl of soup.
4 scallions, washed and trimmed to 4" long
Using Silvermark Herb Snips, snip the scallions into thin slices over a medium sized bowl. Stir in the mayonnaise, Dijon mustard, salt and pepper. Mix well.
Add boiled eggs to the bowl. Using the Toss and Chop Salad Chopper, chop the mixture together until combined. Add the trimmed watercress leaves and continue tossing and chopping until the watercress is chopped and well integrated. Stir in frozen peas and serve.
Yield: about four half-cup servings
*Can substitute arugula
The peas take about a minute to thaw at room temperature. This salad can be prepared a day ahead and kept well covered in the refrigerator.


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The Creston/Orient-Macksburg wrestling team easily rolled past Chariton, 60-13, here on Thursday for senior night in the Panthers' final tuneup before hosting the Hawkeye 10 Conference Tournament on Saturday.
Head coach Darrell Frain said he saw some encouraging things on Thursday, even in some of the team's losses.
"We had a few forfeits along the way, but for the most part, we wrestled pretty well," Frain said. "Even on some of our losses, we closed the gap on getting beat a little bit, so I think we wrestled really well."
Two of those matches where the Panthers closed the gap compared to previous losses were at 126 pounds with Gage Cheers against Devin Harberts and at 182 pounds with Seth Maitlen against Carter Isley.
Cheers lost an 8-3 decision to Harberts.
"Gage gave up some back points early and got a little behind, but battled back and got some back points in the third, and closed the gap and had an opportunity to turn him again," Frain said. "That's a match we could see again at districts.
Likewise, Maitlen could see Isley again at districts, as well. Isley won a 5-2 decision over Maitlen Thursday night.
"He (Maitlen) got pinned by Isley pretty fast the first time, and he closed the gap at John Harris, and got it to 5-2 this week," Frain said. "We're slowing him down a little bit, but we've got to find a way to score on him in case we see him again."
Senior night
The Panthers honored seven seniors during a senior night ceremony. Seniors on this year team are Gage Cheers, Jake Marlin, Jacob Johnston, Cole Tanner, Nathan Robinson, Katie Stevens and Keaton Hulett.
"It's one of those things you don't realize how many seniors you have until you have senior night for them," Frain said. "A good tribute to them and a good night for them. All the support the community gives to them, just a good night for all of them."
Tanner (220), Johnston (145) and Marlin (152) each picked up pins for the Panthers in their matches, while Hulett (195) and Robinson (285) each won by forfeit.
Hawkeye 10
Glenwood wrapped up an undefeated Hawkeye 10 Conference duals record Thursday night, clinching the Hawkeye 10 Conference championship with a 56-18 win over Harlan.
But, Creston/O-M hosts the Hawkeye 10 Conference Tournament on Saturday, and Frain believes his team has a chance to beat Glenwood and defend its tournament championship.
"In a tournament setting, I think we'll be OK," Frain said. "I think our depth will get us through it. It's a little different than typical years. Lewis Central is down, and you can usually count on them to knock off some Glenwood wrestlers. It's going to come down to some head-to-head matches. I think we'll be right there and have to compete well."
The Panthers hope to be back at full strength by Saturday. Gavin Leith sat out Thursday's dual against Chariton with the flu.
Frain said some of the key weights will be the lower weight classes for the Panthers, and guys like Robinson, Maitlen and Tanner in the upper weight classes.
"A lot of it is going to come down to our lighter weights," Frain said. "The fact they can score a lot of points on the backside is huge for us. Cole Tanner is huge for us on the top half. Guys that are coming on like Nate and Seth, if they can compete well, we may have a chance."
Saturday's action kicks off at 10:30 a.m.
Creston/O-M 60, Chariton 13
160 — Trevor Frain (COM) pinned Evan Havick (Char), :34.
170 — Adam Baker (COM) pinned Dallas Cady (Char), 1:26.
182 — Carter Isley (Char) dec. Seth Maitlen (COM), 5-2.
195 — Keaton Hulett (COM) won by forfeit.
220 — Cole Tanner (COM) pinned Chris Wright (Char), 1:57.
285 — Nathan Robinson (COM) won by forfeit.
106 — Stone Hammons (COM) won by forfeit.
113 — Kruz Adamson (COM) won by forfeit.
120 — Wyatt Thompson (COM) pinned Nic Ryan (Char), 4:24.
126 — Devin Harberts (Char) dec. Gage Cheers (COM), 8-3.
132 — Tanner Poush (Char) dec. Joey Huntington (COM), 10-4.
138 — Tyler Nance (Char) major dec. Zack Peppmeier (COM), 13-5.
145 — Jacob Johnston (COM) pinned Stephen McCurdy (Char), 2:34.
152 — Jake Marlin (COM) pinned Connor Grgurich (Char), 3:32.


text:
the way luck moves in the pocket is flat out special...that's gonna be one hard damn kid to get a good lick on i can tell ya that...climbs the pocket side steps things in tight spaces...terrific pocket presence and feel for pressure
hoops scoops 2012 season ..."in 2014 ryan tannehill etches his name in stone amongst the games elite qbs"..."ryan tannehill and andrew luck will carry the afc for the next decade plus the way peyton manning and tom brady have this last decade plus"
for the love of god get a real freaking mike already!!!
Jaguars are so awful...they really should have fired their GM last year...just awful talent on that team.
Blackmon not even trying.
This **** is disgraceful
the false start on gabbert at the end of the half was legit...it's a new rule of late and they put it in cause peyton manning made so many hand and body movements presnap signifying him taking the snap...and gabbert definitely shoulder and upper body dipped on the play
Even Mr. Knowitall Mayock is shocked!! Mularky freakin lost it like Coach Rizzi on the horrible call on the kickoff return at the end of our game.


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petfood.com > Dog Supplies > Crates & Travel Gear > Crate Accessories > Item # 110426
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When we choose to sell these items for less, we display the sale price only in the cart. To see the price, click Add to Cart.
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By Peter BeckerMore Content NowAsk almost any child in Western society about what the North Pole means to them and you will likely hear it is the abode of one Mr. Claus; the domicile of eight or nine reindeer, an assortment of elves and Saint Nick and his family; where Kris Kringel "hangs his hat."
HILLSDALE — Cynthia A. Liddy, 52, of Hillsdale, passed away Dec. 13, 2014 at home under the care of her loving family. She enjoyed fishing and playing pool, she was a loving mother and a beautiful daughter.She is survived by her...
In this week's edition of App Finder, we highlight the 2014's most popular iPhone apps. Check out the App Store for more on each app, and stay tuned for next week's roundup of 2014's most popular Android apps.
By Suzette Martinez StandringMore Content Now
By Ed SymkusMore Content Now
Ranging from chili kicking to beer infused, these truffle recipes are far from ordinary.
[WARNING: The following story contains spoilers about Thursday's Season 5 finale of Covert Affairs. Read at your own risk.] Thursday's Season 5 finale of
[WARNING: The following story contains spoilers from the series finale of USA's White Collar. �Read at your own risk.]
Boston Marathon bombing suspect Dzhokhar Tsarnaev returned to court Thursday for the first time since he was arraigned in July 2013, and he received a shout of encouragement from the mother-in-law of a man who was shot and killed while being...
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I thought this was a post was going to be about physically removing the memory card. It's too late, I'm gonna search for my bed.
Good writing, keep up the good work.
There's a lot of memory installed in my BlackBerry. The Torch 9800 comes with 4 gigabytes of internal memory, and I have a removable 16 GB microSD card installed as well. Until recently, those cards were rather full. My BlackBerry goes where I go, so I usually have it filled with movies, music, ringtones, and anything else that strikes my fancy. My smartphone has so much memory available, I've stopped carrying USB thumb drives. Why would I when my BlackBerry can hold so much?
The Mass Storage mode on the BlackBerry is an extremely useful tool. Using a standard micro USB cable, I can attach the device to any computer and use the smartphone as an external drive. Since the BlackBerry uses standard USB drivers to connect, modern computers have no trouble connecting to and using the memory card(s) on the smartphone. Your BlackBerry will appear as one or more removable drives; you're able to transfer data to and from the device just like you would with a USB flash drive.
When my BlackBerry is attached to the computer as a drive, I'm a fanatic when it comes to that whole Safely Remove Device thing on Windows PC's; I'm sure there's a similar function on Mac's. I always worry about delayed writes or something else causing problems with my memory card if I unplug it too early. It's happened to me before, so I try to be careful. Before detaching my BlackBerry from the computer, I always make sure to check that it's safe to do so.
So what happens when you want to remove or replace the microSD card from within your BlackBerry? Though some may argue this a useless step, I find it's a good idea to turn off my removable memory card first. That way, I can be certain that no data will be read or written as I remove the card. The process is slightly different depending on your OS.
Keep your memory cards safe and happy; let them know when you're going to disconnect them. Whether it be from a computer or from inside your BlackBerry, microSD cards - and memory cards in general - work best when you give them a bit of warning. Suddenly removing an attached drive can have some negative consequences, so play it safe when it comes to your removable storage.
I thought this was a post was going to be about physically removing the memory card. It's too late, I'm gonna search for my bed.
Good writing, keep up the good work.
I pulled mine out(err.... memory card), and put it backin just cause i was checking something hadn"t touched phone for while beforetouching it, put it backin and had it currup upon reinserting it.
I always find these post informative. As much as I use my BB as a portable storage device, its never occured to me to remove it "safely". I usually just plug in, add whatever I need and unplug. Great post
Quick question for the more experienced. Just upgraded from a Storm to a Torch, where the best of my music collection is. Can I just move the 16Gig card from Storm to Torch, or will the differences between OS5 and OS6 make it unuseable?
I may work ok but you may have issues. When I upgraded to a device with OS 5 on it to the Style (OS6) I did a copy onto my computer of the whole card. There is a difference in how some of the folders are structured and arranged especially with the pictures and camera folder. Then I loaded the blank card in my new Berry and plugged it into the computer and put the appropriate files in the proper folders that the new Berry had created. Not a big difference but a good idea to do this non the less. It's a little bit of a hassle, but just think, only need to do this once per new phone. I don't use desktop manager for the SD card. But be sure to back up your device too with Desktop Manager and then you can restore the back up file to the Torch. That's how I did it anyway...
Anyone know if it's possible to plug a flash drive into a BlackBerry's USB port and write to and from it? I've often been in situations where I wish I could have connected a USB flash drive to my BlackBerry and copied data to it, or gave files to a friend, and there was no computer near.
I actually have the proper adapters to do just that and I've tried it. Sorry to say it won't work. Would be great to see something like that on future devices though. I can think of times where it would be quite useful. Also tried this on the PlayBook and it's a no go there either. Would be even more useful on the PlayBook if one had movies they wanted to watch but not use up the memory on the device.
I guess it's slightly misleading that BB's microSD cards are "hot swappable". Yes you can, but....
Good post. Glad I read it. I usually don't do a lot of data transfer to and from my device, but its good to keep in mind.
Good post. Tks for the info.
Good Luck
On my BBS2 9550 (running os5 of course), removing the memory card is very simple and there is no need to turn off the device. Go to Options/Memory and click the menu icon on your device select & click "Remove Media Card". From there is a matter of opening the back of the BB and removing said card. No need to turn off my device. I've been doing this for over a year and have been using the same sd card since I the day I bought my BlackBerry. :-)
I have the same ability on my 9700 running os5. (OS 6 problems caused the downgrade)


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I am thinking of approaching a par three ball golf course in Duluth with a business plan to overlay a pay for play disc golf course on top of their already functioning ball course. While it would be an open course (no woods) it could be made very challenging with OB (greens, sand traps and lakes) and mandatories for most, if not all of the holes. The neat thing about it is that it is lighted and would allow play at night.
I need to be able to convince the owners that disc golf could be profitable for them on days and nights that are slow for ball golf. Is there an interest in the Atlanta disc golf community to support such a course? If so, I need to conduct a poll to see how much interest there is and what we would be willing to pay to play a round both during the day and at night. While I do not know when their slack time is for ball golf, my reality check, at this point, says ball golf is strong for most days and maybe even weekend nights. Weekday nights are where I see the potential to bring them new customers. Before I approach them, I need to gather some demographic information on the number of disc golfers in the Atlanta area. Does anyone have access to that kind of information?