text:
- Kaleb Roberts
- Gladstone
- Australia
This conversation is closed.
Why do people find the need to entrench themselves in rules and policies?
For me, whenever anyone (especially those in authority) say "Kaleb don't do that!" I feel the instant desire to do it. Although desire probably isn't an accurate synonym. It's more like I have to do it or I'll explode. I've been this way for as long as I can remember.
Now that I have entered the workforce, I find there are so many rules and regulations. Granted, some of these have real merit (such as the recycling policies, and earwig steel cap boots when you enter the workshop)
However, there are some rules that are just plain idiotic. What are some examples of this behavior, why do people do it? Is it because (This is my assumption) they are afraid of the unknown? They are afraid of taking risk? Or is something that happens during the "nurturing" phase of life with overprotective parents. Maybe it's even a genetic thing.
Jan 17 2013: It takes things that are high entropy and creates low entropy
Jan 19 2013: I could't agree more. Just rearranging your words:
Rules and Policies reduce entropy in a social system.
The long version:
Rules and Policies, provide a predictable behavior for most of the society members, which reduces the risk of errors and accidents, which also leads to a better decision making for all.
Kaleb, I understand your point, yes there are idiotic rules some people follow blindly, however bear in mind: accidents are caused by unpredictable drivers, employees, people walking in the street and even by unpredictable software. Rules and Policies make things predictable. Predictability means safety.
Jan 19 2013: Excellent point George QT, but some rules are specifically made to limit the freedom of others whom the rule-makers fear, thereby punishing the rule maker with a shrunken world and and those unjustly affected by the rule or policy a shrunken world of opportunity.Jan 7 2013: Clearly Texas is the place to be from.Jan 7 2013: Sometimes rules are brilliant. When I studied music in college they showed us rules for various styles of music such as classical and jazz. The rules were different and complex in order to compose convincingly in those styles. Those rules were invented and collected and passed down from mentors to students. Diminished chords (tri-tones) for instance were considered music from the devil and the church wouldn't allow them at one time (rule). But rules are inherited and they represent lessons from our history and are meant to be either keys or shortcuts or cautionary codes of conduct. There is one rule that I learned in music college and it is "Support The Melody", and inside those three words there is a universe of wisdom involving music arranging; three simple words with so much meaning and guidance that when I realized it's wisdom, it made me smile. Or you could choose to ignore all of musical history and invent your own new style. But your new style will probably have rules and if people didn't follow your rules then it wouldn't be authentic.Jan 7 2013: Some rules are good and the fewer the better the rest are just there to make MONEY!!
Jan 7 2013: This resonates with me Daniel. We put up with a lot just to make a living. Companies impose rules on us and the trade off is the paycheque at the end of the week. Also I see this in business as large stores want everything to be politically correct. The theory being that if they offend no one then everyone will return to their store and make them optimum profits. All about the money. In trying to please everyone sometimes the individual rights can get trampled, like happy holidays vs. merry christmas.Dec 23 2012: Hi Kaleb - In the beginning there was the agricultural revolution and the first human settlements - close knit societies depended upon co-operation, so naturally rules followed this need. (and most of our societies other ideas such as money & institutions)
However, I believe we need only one rule - "don't do unto others what you would have them do unto you." I try to apply this and have introduced my three children as a simple philosophy for life.
Normally, as I also have an instinctive dislike of authority as it often represents unfairness or stupidity or power concentration, I try to follow the following:
"Obeying their rules only encourages them to create new ones. Disobey as often as possible: for gain, for sport, for the art of it." - Ethan Mordde
To create a 'good' society we need just one thing - that is to make sure ALL of our children are trained in the ability to 'think'. This is because if they can really think, we shall unshackle them from unfairness and stupid rule makers. This is I believe the worlds greatest challenge for long term success, and is clearly a difficult one since we cannot even manage to feed many of them presently.
See: Super Fast Track Education:
If you make people think they're thinking, they'll love you; but if you
really make them think, they'll hate you.
Dec 25 2012: "Obeying their rules only encourages them to create new ones. Disobey as often as possible: for gain, for sport, for the art of it."
JP that is one of the absolute wisest things I've ever heard, sincerely, thank you.
"don't do unto others what you would have them do unto you."
Your kids will be way in front of the eight ball I believe. If people could learn that, this society, especially towns like Gladstone would be SUCH a better place... how can you teach people that lesson.. rather, not teach them it, but for them to willingly want to learn it.. you've given me a LOT of food for thought.. thank you.
Linda Taylor
Dec 22 2012: Kaleb, welcome to the grownup world. When we are young we want to grow up so we can make our own decisions and have our great ideas. Then we grow up and realize we are still not there.
- +1
I can tell you that this is a time of transition and learning. You have to reframe the experience of working in a sterile rule driven world before you can understand how to innovate in it. It is a time of learning about the structure and function of the corporate environment in which you work and how to properly innovate with in that structure. If you just go around innovating and changing stuff, you will be labeled a maverick.
Never underestimate the experience of others. They may have seen your great ideas a time or two. They may know it won't work. But some things are simply tradition. But tradition needs to be respected. It is there for a reason, to make work a comforting place to be.
Then there is stuff that has to change. But you cannot change anything until you have the big picture and can see the ramifications of the change down to the guy that mops the floors. You need to learn to work WITH your manager instead of against him. That will get you nowhere. Don't ask why a rule exists, but what can I learn about this. You will eventually learn enough to inspire change but that will not happen just because you got a job. That level of trust and respect must be earned.Jan 20 2013: Racial segregation was a system derived from the
efforts of white Americans to keep African
Americans in a subordinate status by denying
them equal access to public facilities and
ensuring that blacks lived apart from whites.
During the era of slavery, most African Americans resided in the South, mainly in rural areas. Under these circumstances, segregation did not prove
necessary as the boundaries between free citizens
and people held in bondage remained clear.
Furthermore, blacks and whites lived in close proximity on farms and plantations and geographical isolation made contact between neighbors infrequent. However, free people of color, located chiefly in cities and towns of the
North and Upper South, experienced segregation in various forms. By the time the Supreme Court
ruled in Dred Scott v. Sanford (1857) that African
Americans were not U.S. citizens, northern whites
had excluded blacks from seats on public
transportation and barred their entry, except as
servants, from most hotels and restaurants. When allowed into auditoriums and theaters, blacks
occupied separate sections; they also attended
segregated schools. Most churches, too, were
segregated. Reconstruction after the Civil War posed serious
challenges to white supremacy and segregation,
especially in the South where most African
Americans continued to live. The abolition of
slavery in 1865, followed by ratification of the
Fourteenth Amendment (1868) extending citizenship and equal protection of the law to
African Americans and the Fifteenth Amendment (1870) barring racial discrimination in voting,
threatened to overturn the barriers whites had
erected to keep blacks separate and unequal. Yet
the possibilities of blacks sharing public conveyances and public accommodations with whites increased during the period after 1865.
Blacks obtained access to streetcars and railroads
on an integrated basis. Indeed, many
transportation companies favored integration
because they did not want to risk losing black
business.Jan 20 2013: in answer to the main question, if we don't do so what we have done is give the government free reign over us and our choices.Jan 17 2013: Rules are created for a reason and if you want to be able to successfully plead for a change you need to first understand exactly how the system works and why specific rules are in place. Rules have the merit to standardize behavior and activities that work out but at times you come across rules that were put in place for reasons that no longer exist but people still follow them for being rules; a lot of traditions follow the same pattern; I have even seen rules put in place at work to control one single person's behavior and to avoid pointing fingers they made a rule for everyone; the rule still stayed in place after the person left. In some cases people in position to do so make a rule, just to make their life easier, by not having to deal with certain situations or tasks that would occur without the rule.Jan 17 2013: this is an absolutely brilliant question i'm glad you've asked it!
there are of course evolutionary reasons; we are better off for learning from the experience of others rather than being maimed or killed in finding out for ourselves, which necessitates that these others speak up when they feel they have some knowledge that might be useful. therein lies the problem though of deciding whether our knowledge is of high enough standard to pass on or not.
i share your feelings about rules made by idiots! as a teacher i frequently came up against very well-meaning parents but without 5 minutes experience in teaching telling my colleagues how they should teach. thankfully this is balanced by a principal who is well aware that he has spent most of the last 10 years outside the classroom and never imposes rules on teaching policy. in the wider world you've got jim carrey, a legendary comedian who also inexplicably feels it's his duty to speak from a position of absolutely no authority on immunisation.
you might have heard the expression "everyone thinks they are right", and in my experience that is true, however not everyone has reason for thinking so. to give an example, when i have my students retry any test questions they missed, when they bring back their work to me i asked if they got it right this time. some of them just say "yes", and others will say something like "well i looked at the section on this kind of problem in the textbook, and i think i know where i went wrong, and going over it again, i think this is correct now." i believe this is also supported by the really interesting dunning-kruger studies. people in the lower percentage think those above are wrong because their answers are different, and it hasn't occurred to them that they might be wrong, or they'd have checked and learned.
that said though, it is also possible that these rules you are talking about are great rules, but you don't yet have the experience to understand their merit?Jan 17 2013: Rules are the fundamental building blocks of peace. With out rules we eat our children.Jan 14 2013: To properly answer the question I would have to have a list of the idiotic things you thought cause this conflict within yourself. You failed to list any.Jan 11 2013: Assuming we are our own authority, is it a good idea to impose restrictions on ourselves? What does it mean "to do whatever I like"?Jan 8 2013: "Without rules, we're savages."
Can't remember exactly who said this, but I remember it's a quote from the famous drama series:
Prison Break
We need rules.
Sometimes, like it or not, those are shelters for people.
Sometimes those are like cells in a prison.
Because we happen to be weak. We happen to be not perfect.
We challenge the rules as if we're being awesome—actually quite often we are awesome lol.
What's so 'special' about policymakers and Lawyers..?
They have the power we don't have.
We obey the law while trying hard to find faults in it to give ourselves 'freedom'.
We admit its necessity, but we shall fight clever so that we wouldn't be the slaves of it.Jan 8 2013: If you have to do something because someone told you to do the opposite, i fear that is not a helpful quality and might be worth trying to overcome.
Some rules are silly but some are sensible. Like all things in life its hard to work out which is what and often it depends where you are looking at them from . Again do you just hate the rules because you do, or is there a real benefit to changing them and are you able to bring about that change.
Don't waste effort moaning or debating if there is a problem to solve, take action and make the world better.Jan 7 2013: There's nothing i hate more than someone telling me "You can't do this.." or "Who gave you permission.."
When we're all born equal no one has the authority to decide what someone else can can or can't do. I've always has this mind set since i was a kid
But i guess a lot of people like being told what to do
Jan 7 2013: Amen. I have a very similar mind set, I think it's probably the reason I'm always in trouble.
I think if people mad their own decisions the world might be a little smarter. And a little less populated.
Jan 7 2013: But the sad part is that some people are in a psychological prison that makes them accept every rule given by a figure of authority without questions asked no matter how pointless or stupid it may be.Jan 12 2013: Re: "...And a little less populated."
What you said seems to imply that rules may be what made humans the dominating species on the planet. Don't you think, some rules help to avoid mistakes already made before us?
Check out this article
It seems to say that ability to obey rules may be a useful trait.
What do you think?Jan 5 2013: Kaleb, Often rules seem stupid ... In Arizona you cannot ride a horse into a saloon .... yep still on the books. Just last year a idiot did it. I would love to know the real reason this became law. Probably had to do more with the safety of the horse than the idiot riding him.
Most rules are written because someone has done something to harm ... endanger ... to take advantage .... to dominate others through trickery ... etc ...
It is fun to look at the old laws and wonder if they should be stricken from the books. I searched for silly laws still on the books and found some doozies.
My suggestion is to read them .. laugh at them ... and move on to happier thoughts.
What are some of the silly laws in Australia?
All the best. Bob.
Jan 7 2013: Hey Bob,
That's rather odd. People here can be.. well they are pretty idiotic. I'll give you two examples.
A) It is illegal for anyone other than a fully qualified electrician to change a light bulb..
I have yet to figure out why this is, it's quite pathetic really.
B) It is HIGHLY illegal to go within 100 meters of a whale carcass.
This has to be a personal favorite. The reason they passed this law is because a whale beached itself somewhere in Queensland. This attracted many sharks. Tourists and locals alike decided they wanted to pet the sharks (while they were in the middle of a bloody feeding frenzy) so they (they being about 20 people) waded out to the carcass and began to pet the sharks. Needless to say it wasn't the brightest idea ever.
Jan 7 2013: G'day mate, Those are funny and the petting sharks is stupid I agree.
I hope that is not one of the laws that you just want to run out and disobey .... LOL
Glad we could have a laugh .... thanks for the reply.
Bob "the yank".
Jan 7 2013: Bahhaa Hey Bob "the yank"
I was born and raised in Texas, we only moved to Australia about five years ago LOL
And surprisingly, (and disappointingly) most people don't talk like Steve Irwin..Jan 7 2013: We moved here from Texas where I was an engineer for General Dynamics in Ft Worth.Jan 4 2013: In working in the industrial field, I have come accustomed to rules. These are the federal regulations of OSHA. The power that nature can unleash in the industrial field is sometimes unimaginable, and often have tragic results when rules are not followed. These are the rules that protect people against harm. Because of this, people know that some rules are necessary for well being.
As to your last paragraph, there are two ways of looking at it;
First, there are three kinds of people in the world: those who make things happen, those who watch things happen, and those who wonder what happened.
The people who make things happen understand the difference between good and idiotic rules. They are willing to question idiotic rules, or even ignore them in spite or frustration. The people who watch things happen just go with the flow. Unless the rule is causing problems, they would just assume not making waves. The people who wonder what happened would be lost without rules. They are often the ones to whom idiotic rules apply.
I find that many idiotic rules had a reason in the beginning, but the cause of the rule has changed and nobody wants to question the rule because it means questioning authority or admitting that they don't know the reason for the rule. They follow the rule just to avoid conflict or ending up looking stupid.
The second way of looking at it is this; People who are known to break rules are often bypassed in certain jobs because those jobs require strict adherence to rules (such as when working with high voltage). Not all rules are idiotic even though they may seem to be. It's one thing breaking a rule and getting your hand slapped, or breaking a rule and getting your head ripped off. So some people will follow idiotic rules just so that they don't get into a habit of deciding for themselves which rules they can choose to ignore.Jan 3 2013: Kaleb,
I have moved from entry level positions to executive leadership roles within organizations. When I began I was full of wisdom from the books and school, and my own idealistic ideas. After several disgreements and arguments out of frustrations I was educated further on corporate dynamics, and people management.
Usually there are very good reasons for some stupid rules in some organizations. And some such stupid rules cannot be explained to some staff due to reasons concerining security and trust. There are many good reasons and good rules in place as well for the overall benefit of the organizations sustainability and growth which may not be favorable to some individuals or positions within a company. Often there is a reason for the madness. Running an organization involves accomodating very complex situations. namely: people management, allocating financial and human resources, legal requirements, allowing people to take good and bad decisions, providing autonomy to individuals, and restricting some decision making powers as well. All of these eventually are driven for financial reasons and secure, stable growth plans. Yes risk taking is a major concern as well for all organizations. Although it may seem that some organizatiosn have taken risks, they are usually very carefully planned and tested ones.
Having said that there are some organizatons with poor manageres and power holders. They take bad decisions and cover up thier mistakes with authority and arrogance. But such peopel do not last for long and are eventually let go. Companies that do not take the time and trouble to identify such bad managers will suffer dire consequences and fail. If you are in such an organization and you are sure of this, then you are free to opt out and look for a more stable and good company.Dec 31 2012: Rules provide consistency across an organization and across time. Rules are necessary to coordinate the efforts of large numbers of people. It would be absolutely impossible to run a large organization without having policies, procedures and rules. If everyone made their own decisions on every individual issue, an organization would have no consistency whatsoever. As an individual, you make rules/policies/guidelines for yourself to guide your own decisions so they remain consistent over time.
As with everything human, problems arise for many reasons. Rules are often based on a particular situation at a particular location and time, and the rule is applied broadly, but it is not completely suitable for other locations. Rules often remain in effect long after they have become obsolete. Rules made at the top of an organization are often misinterpreted by people at the lower levels. I once heard a vice president of a major corporation lament that a rule he had initiated had been misinterpreted and had the exact opposite effect of what he had intended.
Often the people at the top of an organization place little or no priority on the policies, procedures and rules that guide the behavior of everyone else in the organization. The most competent people are too busy with more important matters, so maintaining the policies and procedures manual is not done very well, and often does not reflect the actual wishes of the top management.
Life is messy, and our rules reflect the limitations of humans.Dec 31 2012: I think if there were no rules mankind had not achieved any degree of development. Probably we had only achieved disorder and very low efficiency. I agree there are a lot of absurd rules, but on the other hand there are many of them useful and helpful anyway.Dec 27 2012: Kaleb I think you of what r saying share more of what you feel and how you thunk we can go forward
Dec 27 2012: I feel very.. strongly? About this.. it's something that's bothered me since childhood. I think SOME rules are necessary. The sheer amount of them creates disasters, less empathy, less kindness, poorer human relationships. With a few less rules, and a few SMARTER rules, with a huge increase in common sense, I think that's how we should move forward. Unfortunately, I don't have a clue how to distribute common sense to the populations of the world.Dec 26 2012: There are many types of people in the world. There are those who make and question the rules, and those that follow and enforce them. People entrench themselves rules and regulations because they need the stability and support of something concrete like rules and laws. For them, it is something that will always be here, a constant. Rules provide them with organization and a guideline of how to live their life.Dec 25 2012: People require rules because they are afraid, if you leave someone to the unknown they either accept it with a daring and evolved mind or they panic and deploy rules out of anxiety. Anxiety is a form of mental illness so of course obsessive compulsive actions and paranoia take over, thus people who are more accepting and willing to explore are punished by the small minded. The ones who tend to be the most rule and law abiding tend to be individuals who claim to be politically correct and morally upstanding, they are neither and are living in a dream world quite frankly.Dec 24 2012: This question kind of reminds me of Golding's, "Lord of the Flies". If we had no rules whatsoever, someone would start enforcing rules anyway. So I think it is innate behavior for us to live by rules. Although I do have to agree with you, there are a lot of idiotic rules out there lol.Dec 24 2012: I believe it's because many are afraid to make a decision for whatever reason. Rules lay it out so no decision making is required. I like making decisions so I too balk at many rules.Dec 23 2012: Because as a society we have been bred to feel that rules are for the better, that if there were no rules we'd all lose our morality and run amock and do as we please, that we would lose all integrity.
If you look at it through a bigger picture, it is easier to control any people when you run them down with rules, as they won't have time to question what you are doing.
In regards, to genetics, I don't know, but when I'm told I shouldn't do something, unless it's bad for the humankind or puts someone's life at risk, it makes me want to do it, just because I don't feel that anyone has a right to tell me what I can or can't or should or should not do. If I am following my moral code, then why in the world can I not do something ?Dec 23 2012: Because rules are often useful and beneficial. They often increase productivity, help to produce predictable results, and facilitate cooperation between people in an organization (or even a household).
As an analogy, think why people use railroad which greatly restricts movement of the vehicles.
Typically, rules are based on some mutual convention or belief like "If A then B". If "B" is a desired result, the rule may be "Do A". If "B" is an undesired result, the rule may be "Don't do A". These rules make sense as long statement "If A then B" is true. It may not be always true. Or "B" may no longer be a desired result. It's important to realize when the underlying statement is not applicable to decide when rules do not apply or need to be changed.
Statements "We should follow rules" or "We shouldn't have rules" are, themselves, rules with limited application.Dec 23 2012: I had and still have the same phenomenon as you described in the first para of your post above....
Well I feel we human being are unruly that's why we need rules.....!!!!
Look in to the history......say until we had rules about racism / gender equality how we acted ?Dec 23 2012: A basic human need is to belong. Rules help people know the right ways in which to behave so as to belong. I think it's fair to say that most people will tend to follow the rules as culturally speaking that is the way in which they have been taught or have learnt to behave over time. Some personalities however, have evolved differently; they prefer to challenge and question the status quo and growing up in a country like Australia there is sufficient credibility given to this behaviour to encourage it; but only to an extent. I think it's fair to say that you and I would have been alike in this regard until I moved from Australia a number of years ago and lived in a country where questioning the rules was simply NOT done. It took me many years to become accustomed to not questioning and simply following the rules as at the end of the day it was in my best interests to do so. I no longer live in that country as a result; the rules had to be followed and to not follow them created such a difficult situation that it would only be detrimental to myself; often hugely so. So I learned to follow the rules even though I recognised that they were simply idiotic. I learned to take a queue number at the bank to be served even when I was the only one there as without it I would not be served; a simple but real example. Unless the population wants change, change is not likely to happen.
I now live in a country where rules are treated differently. I come back to rules being necessary to help people know the way in which to appropriately behave so that they can feel that true sense of belonging within that community. Some need this more than others.
Questioning rules is neither right nor wrong ... it can be about change or stamping one's signature. I see that what I learnt from living in this different country hasn't made me less of a strong willed person; simply a more accepting one. Some things are worth changing ... direct your energy into making a difference there.Dec 23 2012: I'm going to get p.c. grief for this: your manager is a female; females by nature prefer security over innovation. That's not to say females can't innovate; it's just a more masculine thing. You don't say what business you're in; can you, in your spare time, put some of your ideas together and try them out, or do they need to be inextricably tied into an established system? Believe me, I know where you're coming from; as soon as I hear a rule, my first reaction is, "OK, how can I get around this?" Can you put this together so you can break off and become a competitor to your current employment? You'd have the advantage of knowing your competition and their weaknesses, plus, possibly, her client base. If you can offer better product and services for less $, you might be able to shoot out ahead of her. Best wishes to you; but, innovators have always had it hard. I guess it's a Darwinian thing.
Dec 25 2012: It would be brilliant to be able to "Shoot ahead" Unfortunately, I work for a Toyota Dealership at the moment. I quote my manager here when I asked if I could try and cut down customer wait times by making our current systems more accurate and defined "..Kaleb, you cannot do that because it is not the Toyota way..".As much as I would love to own my own business, believe me I dream of it constantly, around the world the Toyota Production System is considered one of the best, I'm trying to learn every facet of it so I can adapt it to my strategies. Or visa versa.
Unfortunately, people at this level of the game don't seem to understand anything about it, except for that there are a lot of Japanese words that are difficult to pronounce.
The only way that myself and the other frustrated people around here can rise over the incompetent people (at least the only way I know of) is to spend a year or two working like there's no tomorrow, and to always do our best.. eventually, the current managers will make one mistake two many, lose a giant contract, something like that, and only then will there be an opportunity to shine . Or to quit and follow my passion (which at this point is still undiscovered)
Admittedly though, I like the way you think, and thank you.Dec 22 2012: Most people (perhaps all people) do not fully understand the possible outcomes of their stupid policies or laws.
For example, I recall a policy in a local telephone company that measured the effectiveness of customer support by measuring the number of question answered per employee. But rather than count the number of questions, they counted the number of phone calls. The resultant behaviour proved annoying to the public because to get the count, the customer service representative would only answer one question per call. To ask two questions, you had to call twice.
Sometimes the law is just not thought through well enough.
For example, on Waldron Island Washington State, building code states that no building structure shall contain more than two potable water toilets. This is mostly for conservation of water. It's a stupid law because it doesn't prevent people from going to the bathroom any less frequently and therefore accomplishes nothing.
So the real answer is that by and large, we are as dumb as dirt.
Dec 31 2012: "dumb as dirt"
I have never seen dirt hurt itself.
Dec 31 2012: I'm not sure what part of the country can claim that saying. We also used to say someone was a dumb as a sack full of hammers or bag of cookies. In any case none of the above can carry on much of a conversationDec 22 2012: Rules and policies helps in keeping teams and communities on track in terms of focus and vision.
If there are no rules or policies, and if people can just do anything anywhere at anytime, the chances of making any worthwhile progress are so slim.
And rules/policies are not about individual comfort and convinience or opinions; if it were such, no nation would have a constitution because it is impossible to create a document that makes everybody 'happy'.Dec 22 2012: Rules and policies helps in keeping teams and communities on track in terms of focus and vision.
If there are no rules or policies, and if people can just do anything anywhere at anytime, the chances of making any worthwhile progress are so slim.
And rules/policies are not about individual comfort and convinience or opinions; if it were such, no nation would have a constitution because it is impossible to create a document that makes everybody 'happy'.Dec 22 2012: You might find some insight into this question by exploring the origins of rules and policies.
Try thinking about questions like these: What was the first rule? Who made the first rule? Why? Who did it apply to?
Also remember that everything that is necessary is taken to excess by someone, and it can be very difficult to determine the point when something becomes excessive. Would like to add more, but time does not permit.Dec 21 2012: Rules, policies... These are not innately bad things, they are up to people to decide what is good or not, it's up to every individual to figure out why.
Sometimes, the group has more influence than oneself does, and that results in non-reflection of some of the actions we do. . .Dec 21 2012: I feel the same way you do, and I've struggled with it all my life. There are two basic ways to think: in the box, and out of the box. We need both. In the box is our starting point; the good majority of in the box rules and procedures are there because, over the years, it's been found that they work, or at least make for safe and productive working. But . . . in the box has hardly ever lead to innovation and new developments. Therefore, provisions must be made for out of the box thinking. The truly smart person knows when to think in the box, and when out. Both are vital to the progress of civilization, but must be done wisely and in their proper order.
Dec 22 2012: I think you're right although I'll never admit to it.
People here, are all robots. In this town, that's all anyone is. There is no current space for innovators. No space for educated people either seemingly.
Maybe I'm just young and foolish, but how do you know when to think in and outside the box? I've always had trouble being contained, and yet it's the only way people treat me. Like I'm some animal who needs to be caged, because they are afraid of what I could do. I want things to be in constant improvemnet and change. My manager, thinks we should stay exactly where we are. Forever. How do you persuade her that, simply put, that's not how a company should be run? Needs to be run? We will never get anywhere if we do the bare minimum.
Help?Dec 21 2012: What kind of work do you do? What are the rules you believe are "just plain idiotic"? I suppose if you think a rule is idiotic you can ask your supervisor why the rule exists.
Dec 22 2012: I'm working for a Toyota Dealership while I'm studying at University.
The rules are little things that were put in place so certain people don't confuse themselves while tying their shoes. Because strangely enough, there are people here (Older than myself) who do not know how to tie their shoes. No exagerations.
I've asked my direct supervisor some of these questions, and she told me it was a Dealership thing and to ask the owner. So I did, he told me it was a toyota thing, and to call Toyota's Head office. So naturally I did, and they still had no idea why these rules are in place. So I called head office in Japan, and am now awaiting a call back.
Dec 22 2012: I appreciate your courage in pursuing your questions up the ladder. I'm sure you'll learn things that someone who doesn't question won't learn.
Still don't know exactly what the rules are you disagree with, so it's hard to comment on whether they're valid or not.
In general I like rules. They give some structure for living life. Most of the rules I encounter I see the sense in. When I encounter a rule I don't agree with, when I keep asking about it, I usually find it has some sense to it I didn't see at first.Dec 21 2012: Rules and regulations whether they be in the workplace or in science provide stability, purpose and guidance. In science if there were no rules we would be changing theories on a whim which would make teaching chaotic and the need to revise textbooks every few months a necessity.
Why do you think in physics the rule of thumb is either you come up with a Nobel Prize winning theory before age thirty or you can forget about ever winning one? This has nothing to do with creativity---it is all about the willingness to take risks. When you are young, foolish and fearless, you will take extraordinary risks. Once you are settled in with career consuming vast amounts of time and the need "to bring home the bacon" it is a lot less desirable to take risks. This is especially true in science where to pursue a controversial new theory to acceptance takes decades (Often it means waiting for the entrenched scientists to die off).
From a personal perspective I'm more creative and reckless at age 61 than I was at age 25.
Dec 21 2012: Hi Richard,
I was unable to find a way to send the attachment through TED, but if you message me with your e-mail address, I would be happy to send it directly to you.
Dec 21 2012: I'll have to check the terms of service to see if this is permissible.Dec 21 2012: I would imagine that the degree to which many individuals react to restrictions placed on their freedom to be a function of several things:
1. The logic behind the rule.
2. The amount that the rule imposes upon them.
3. Their own personal susceptibility to "reactance" (the psychological term for the phenomena you described in the first paragraph).
4. The rigidity of the rule; whether spending energy reacting has any risks or is likely to have any impact on the outcome of the situation(s) that the rule is being applied to.
This discussion reminds me of a publication I read earlier this year that addressed the same topic. Do you have any interest in reading it? If you do, I'd be happy to look through my folder of scientific journal articles (of which I've downloaded entirely too many haha) and send it to you.
Dec 21 2012: That would be brilliant, thank you kindly ma'am.
Things like that always interest me.
Dec 21 2012: It's my pleasure. I sent you an email through TED's messaging system but did not see an option to attach files.
Edit: Richard, I was unable to reply directly to your comment, but I left a reply for you above.
Dec 21 2012: Hi Jacqueline!
If you've got a way to get in touch, I'd love to see that article you mention!


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Practice Philosophy
My practice philosophy is to provide my patients with personalized expert care that meets and exceeds patient needs and expectations. I place special concern for the health and safety of all of my patients. My goal is to achieve the finest and most optimal aesthetic outcome for the patient.
Achieving excellent results requires the time and ability to listen to patient concerns and desires. It also requires the surgical expertise and ability to execute these desires and achieve the desired results. You will have the time during consultation to address and discuss your needs, concerns and wishes. Keeping up with the advances in medical technology allows me to integrate the newest, best and safest techniques with a surgical experience, achieving the best and safest results for my patients.
Follow up care is part of the surgical care and will continue until you have fully recovered. Regular and periodic follow up visits are recommended to maintain touch with the office and to monitor your progress.
Plastic Surgery of Breasts
Breast Augmentation
Breast Reduction
Plastic Surgery of Face, Eyes and Nose
Body Contouring
Buttock Enlargement with Fat Injections
Fat Injection
You will not be rushed - your concerns, desires and needs will be discussed. Various applicable treatment options, including risks and complications will be addressed. You will be examined. During the exam, surgical scars will be marked and an opinion will be given about what I feel will provide you with the best outcome. Pre and post operative photographs can be reviewed.
I always like to see patients at least two sessions prior to surgery. I request patients to go home, take the time to assimilate and review the information discussed which can be lengthy and long. Usually further questions or concerns may arise. Please make a list of these questions and bring them with you on your next consultation so they can be addressed.
If your procedure is medically necessary and partially or completely covered by insurance we can and will contact your insurance company to help facilitate your care.
I have two offices. The primary office at 1035 Park Avenue is on the corner of 86th Street and Park Avenue in New York City. The second office is in the beautiful Brooklyn Heights area, close to downtown Brooklyn near Court Sreet and Montague Street.
The offices are state of the art, immaculate and clean. The finest and best has been used to equip and furnish both offices. The Park Avenue office has an accredited operating room and recovery room. I have AAAASF accreditation. Patient health and safety is of maximal concern and nothing has been spared to ensure this. You can have your procedure in a safe and private beautiful space with private nurses to monitor you before, during and after surgery.
Location & Office Hours
Suite 8A
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Brooklyn, NY 11201
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Monday - Friday, Available for Consultation By Appointment Surgery schedule available for Saturday
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New York, NY 10028
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Payment Options
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subset: commoncrawl


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For Immediate Release Media Contact:
March 4, 2014 Elise Shrock, 317-232-9496
Phil Johnson, 317-232-9404
Sean Mobley, 317-232-9507
Unstudied tax measure passes amid local impact concerns
INDIANAPOLIS—On Tuesday, a measure that would cut the corporate income and business personal property tax cleared the Indiana Senate by a vote of 33-15. Senate Democrats expressed serious concerns with enacting a proposal that would potentially cost the state hundreds of millions of dollars annually without first thoroughly studying the impact on local governments and schools. They pointed instead to investing in early childhood education, an investment that would have long-term economic development benefits and help develop tomorrow's skilled workforce.
Senate Democratic Leader Tim Lanane (D-ANDERSON): "I voted today against House bill 1001 which allows major tax breaks…" http://www.in.gov/legislative/senate_democrats/files/audio/Lanane.HB1001.030414.Priorities.MP3
(Length: 00:33)
STATE SENATOR JOHN BRODEN: "I am very concerned about the tax shift. It cuts property taxes for businesses, but then it increases property taxes on individual homeowners …" http://www.in.gov/legislative/senate_democrats/files/audio/Broden.HB1001.TaxRevLosses.MP3
(Length: 00:35)
House Bill 1001 will now be returned to the original author of the measure to consider changes made by the Senate.
Sen. Broden represents Senate District 10, which includes the cities of South Bend and Mishawaka, and portions of Portage, Penn, Centre and Clay townships in St. Joseph County. For more information on Sen. Broden, visit www.IN.gov/s10.
NOTE: A photo of Sen. John Broden can be downloaded at http://www.in.gov/legislative/senate_democrats/images/headshots/Broden.jpg.
Home | Senators | Legislative Information | Issues | Caucus News | Internships | Staff | Contact


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Guide to Marrakech
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Marrakech Mint and Herbs
One of the delights of Magical Marrakech is a traditional mint tea which is usually made from seven or more mint and herb varieties. What could be more refreshing as you relax in your Marrakech Riad. The video above shows … Continue reading
Posted in History and Culture, The Marrakech Medina, Uncategorized Tagged herbs, Luxury, Marrakech, mint, mint herbs marrakech, mohammed, rak, relax, riad marrakech luxsus, shops, souk, tea shop marrakech Comments Off


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Tuesday, January 22
7:00 p.m. EST - Governor Pence to deliver State of the State Address.
*Media are welcome to attend. Journalists who do not regularly work in the Indiana State House but would like to cover the governor's address from the House Chambers are asked to contact Tory Flynn, Communications Director for the House Republicans, at 317/232-9887 or email@example.com, to arrange for access.
Indiana Statehouse, House Chamber, 200 W. Washington Street, Indianapolis, IN
The State of the State address will be available live on the Internet. It may be accessed from the state website: http://www.in.gov/gov/2013stateofstate.htm. The stream will also be available on mobile devices.
Any updates to this schedule will be posted to www.in.gov/gov. To receive automatic email alerts regarding additions or changes to all calendar events or press releases from the Office of the Governor, please click here, search for "Governor" under "State Agency" in the left column, and sign up by clicking "Subscribe" in the top right corner of the page.


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I realised over the years that on a 14 inch blade only the firts 2 inches are made of decent abrasive...
Is that because there are more fibre the closer you get to the arbor or because no matter the constant rpm of the saw a 10 inch blades has much less inch per minute of abrasive working on the surface... 14 x 3.1416 x rpm = ipm when it's reduced by 40% and fibers are added...
Over the years we tried so many brands even the walter representative made me try his whole assortment with limited failure or success. Add to that that some (most) cheaper blades are of poor quality and reinforcment inside is not always center and never cut straight, i have a home made saw driven by a 5 hp motor wich work best with mid priced blades (1/4 x 6 flat and straight when blade is worn less than 10 inches, or 3/8 x 2 1/12 flat cold rolled 4 stacked).
Performnce wise, not bad! but the dust.... I plan to go dry cut but fear the noise...
Results 11 to 14 of 14
Here's the physics:
The cheapo wheels use abrasive grit that melts VERY NEAR the melting temperature of the target metal (steel, usually), or grit with impurites that melt BELOW steel's temp. So when the kerf gets hot, the cheap grit liquefies instead of the metal, but the grit sticks to the wheel (UNlike the steel flakes, which oxidize & shrink rapidly as they go around the backside of the wheel & flake off), so you make no progress. If you have a diamond truing tool for your bench grinder, you can use that (or maybe a common quartz stone) to break the glazed grit & expose more fresh abrasive, but the only real fix is to buy high-quality name-brand wheels.Walk softly & carry a BIG SIX ! ! !
01-30-2013, 12:12 PM #13Senior Member
- Join Date
- Sep 2009
- Location
- Colorado
- Posts
- 238
Let the wheel cool
I find that the best way to prevent the problem you describe is to not let the wheel get too hot. If I have a big cut, I will pull down for a few seconds, let up a few seconds, pull down a few seconds, etc. Ever since I started doing it that way, I no longer have the problem you describe.
I will say that 1 inch square is about my limit for the abrasive wheel. If I have to cut 1/2 inch by 1 inch, I put it on edge, go up and down until I get through it. They work a lot better for 1/4 inch thick material, but always put the material on edge. I think that I could probably cut 2 inch by 1 inch material by putting it on edge, and just taking my time.
I use my abrasive wheel a fair amount, so I have some experience.
My two cents.
01-31-2013, 12:05 PM #14Member
- Join Date
- Nov 2010
- Posts
- 42
Thanks for all the responses....some good advice
The "blade" that mad me write this thread is Pferd. made for steel.
I have been using this cutter about 20 years. I have already been using many of the methods described here. One I have not tried is setting the materiel on end.
I have about 200 (see them on craigslist all the time, I usually pay less than a dollar each) of these abrasive cutoff wheels of various brands. Some deteriorate faster than others but they all seem to "clog" about the same.
I agree that is it is a noisy messy not precession cutting device, but for cheep cutting it is the way to go.
I also have a Milwaukee 14" dry cut........really NICE cuts, I have cut a 9lb 5" channel where drop could only be measured with a micrometer, but the blades are EXPENSIVE!
I would list all my tools but you guys would just cry.
Thanks for all the replies!


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En español l July is upon us so you might want to avoid the hospital if at all possible.
Why? On or around July 1, newly graduated interns, residents, nurses and other new health care workers first report to work at many of the nation's hospitals, eager to start practicing medicine — on you. No surprise then, that quality of care in hospitals plummets, and medical errors increase, during the following month.
In the most recent study on the so-called "July Effect" — published earlier this year in the Journal of Neurosurgery: Spine — Mayo Clinic researchers analyzed outcomes of 1 million spinal surgery patients admitted to 1,700 hospitals across the country during July over an eight-year period. Roughly half got surgery at a teaching hospital with newly minted medical students, while the others were treated at nonteaching facilities.
Their conclusion: Patients at teaching hospitals fared only slightly worse — a "negligible effect," wrote the researchers — on criteria such as in-hospital death rates and negative reactions to implanted devices.
"But they also found that compared to others, July patients had higher rates of discharge to other long-term care facilities, as well as post-operative infection and surgical stitches failing," notes David Sherer, M.D., a practicing anesthesiologist and past director of risk management for a large insurance provider, who wrote the book Dr. David Sherer's Hospital Survival Guide.
"Those are three significant outcomes that certainly don't convince me the July Effect doesn't exist," adds Sherer, who was not involved in the Mayo analysis. "And the fact that researchers keep looking into this, asking, 'Is it real?' makes you believe that there's good reason to believe it is. Based on my own experience and speaking with other doctors and patients, I'm convinced the July Effect exists."
Which hospitals are riskiest?
When analyzing the research, it turns out "where" may be even more important than "when." Most studies indicating patients problems during July find higher rates at teaching hospitals — those typically affiliated with a medical school where newly minted medical students get their first on-the-job training.
You may get more personal attention, but the skill level isn't there, explains Sherer. "You have newcomers arriving at hospitals — often placed in a sink-or-swim situation — and they don't know where anything is or how anything is done."
Although as a group these newbies are universally supervised, "from day one, residents are writing medication orders and doing certain procedures and diagnostic tests with relatively little direct supervision, so there's always an opportunity for something to slip through the safety net," adds Christopher Landrigan, M.D., who teaches at Harvard Medical School and oversees residents at Boston Children's Hospital.
Next page: Ways you can protect yourself. »


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Celebrate a legendary landmark in the history of the civil rights movement! This amazing poster features a classic black-and-white image of the courageous Selma-to-Montgomery Alabama marchers of 1965, who helped oust segregation from the South once-and-for-all, and whose passion inspired the Voting Rights Act to pass into law. From King's classic quote, to the rare archival image, to the brilliantly colorized American flag, it's all here, the perfect tribute to the Civil Rights movement in America!
Product Specifications:


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HaloLink™ Protein Array Systems provide proteomics researchers a simple way to create medium-throughput custom protein arrays using content of their choice. The HaloLink™ Protein Array Systems are based on the HaloTag® technology, which uses a single protein tag for protein purification, imaging, protein immobilization and protein interaction analyses. To create a custom protein array, proteins of interest are expressed as HaloTag® fusion proteins in a cell-free protein expression system and then covalently captured on a hydrogel-coated HaloLink™ glass slide. A removable, 50-well silicone gasket allows up to 50 different proteins to be captured in parallel for multiplexed studies. Custom arrays can be used to study protein:protein and protein:DNA interactions as well as to perform enzyme assays. The ability to easily create a protein array will allow proteomics researchers to shift their time from creating and troubleshooting arrays to performing experiments.
Nidhi Nath1, Robin Hurst1, Brad Hook1, Elaine Schenborn1, Poncho Meisenheimer2, Mark McDougall2, Dieter Klaubert2 and Doug Storts1
Functional protein arrays allow researchers to probe thousands of proteins immobilized on a surface to detect proteome-scale protein-interaction networks(1)
. Researchers also are increasingly using these arrays in clinical diagnostic applications to look for antibody response to cancer antigens and infectious agent(3)
. The main advantage of using protein arrays is the miniaturization and multiplexing capabilities they provide, enabling large amounts of information to be generated rapidly from small amounts of sample. However, before protein arrays become a mainstream tool for biological applications, several technological hurdles remain to be solved.
Scientists interested in using functional protein arrays for their specific biological applications have limited options. The first option is to use preprinted arrays with specific protein content already immobilized on the surface. However, the utility of these arrays is limited because most researchers want to use their own specific content. A second option for researchers is to create custom protein arrays containing content of their interest, which is a daunting task because of the need to:
The HaloLink™ Protein Array Systems are designed to address the needs of scientists interested in using protein arrays for medium-throughput, multiplexed protein interaction studies. Innovations in cell-free protein expression systems, HaloTag® capture technology and surface engineering are combined to simplify the process of making custom protein arrays. First, cell-free transcription and translation systems—TnT® T7 Quick Coupled Transcription/Translation System and TnT® SP6 High-Yield Wheat Germ Protein Expression System—are used to express proteins directly from plasmid DNA. These systems are easy to use, rapid, and ideal for expressing proteins toxic to the cell line of choice. In addition, they allow protein modification with nonnatural amino acids containing fluorescent or other reactive labels to facilitate downstream detection.
Second, proteins of interest are expressed as HaloTag® fusion proteins in cell-free systems. The HaloTag® protein is a 33kDa tag engineered to form a covalent bond with its HaloTag® Ligand. This tag allows covalent and oriented capture of proteins of interest on HaloLink™ Slides directly from the cell-free expression system, hence eliminating the need for any prior purification step.
Third, HaloLink™ glass slides are coated with a polyethylene glycol-based hydrogel that is known to resist nonspecific protein adsorption and to minimize surface-induced denaturation of specific proteins. Finally, we used a silicone gasket to create 50 wells on the glass slides, allowing multiple assays to be performed manually on the same slide without any specialized equipment.
Figure 1 provides an overview of the workflow for custom array fabrication and downstream application using HaloLink™ Protein Array Systems. To create a custom array, proteins of interest are expressed in a cell-free system as HaloTag® fusion proteins and added directly to wells on the HaloLink™ Slides to capture fusion proteins at the surface. The custom array can then be used for downstream applications such as protein:protein or protein:DNA interaction studies. The combination of cell-free expression systems and HaloTag® attachment chemistry allows creation of a medium-throughput custom array and performance of protein interaction studies in less than 8 hours.
These systems allow the user to create a medium-throughput custom array and perform downstream applications in less than eight hours.
To demonstrate the utility of HaloLink™ Protein Array Systems for multiplexed protein:protein interaction experiments, we studied interactions among eight HaloTag® bait fusion proteins and three prey proteins. The protein interaction study was divided into two steps: First, we expressed eight HaloTag® bait fusion proteins using the TnT® T7 Quick Coupled Transcription/Translation System and captured them to produce the custom HaloLink™ protein array. The HaloTag® bait fusion proteins used in the study were: HaloTag®-cJun, HaloTag®-cFos, HaloTag®-Protein Kinase A (PKA), HaloTag®-R1α, HaloTag®-p53, HaloTag®-p65, HaloTag®-β-galactosidase (βgal) and HaloTag® protein only (control). The second step was to probe the custom HaloLink™ array with three prey proteins for bait:prey interactions. The prey proteins selected for the study were c-Fos, c-Jun and p50.
The eight bait proteins were cloned into pFN19K (N-terminal HaloTag® fusion) Flexi® vectors and expressed in the TnT® T7 Quick Coupled Transcription/Translation System (see supplementary information for details). After expression, 5.0μl/well of samples were added to the individual wells on HaloLink™ slides (Table 1), incubated for 1.0 hour, washed and dried to obtain a custom array. Column 1 contained a dilution series of HaloTag® standard protein. Column 2 had HaloTag® bait fusion proteins diluted 1:20-fold in PBSB (PBS with 10 mg/ml BSA). Both of these columns were probed in subsequent steps using Anti-HaloTag® Polyclonal Antibody (pAb) to confirm and estimate the binding of HaloTag® fusion protein. Columns 3–5 contain eight HaloTag® fusion bait proteins in triplicate for protein:protein interaction studies using the three prey proteins and two additional negative controls (cell-free lysate without DNA, and PBSB-only).
The custom HaloLink™ array fabricated above was interrogated with prey (probe) proteins to identify key protein:protein interactions. Prey proteins (c-Jun, c-Fos and p50) were cloned into TS1K Flexi® vectors (non-HaloTag® analog of pFN19K) and expressed in TnT T7 Quick Coupled Transcription/Translation System (see supplementary information for details). Prey proteins were biotinylated during expression using Transcend™ Biotinylated tRNA. Biotinylated prey proteins, c-Jun, c-Fos and p50, were added to columns 3, 4 and 5, respectively, of the custom array and incubated for 1.0 hour. After washing and drying the probed slide, positive interactions were detected by incubating with Alexa Fluor® 647 labeled streptavidin. In parallel, columns 1 and 2 of custom array were probed using Anti-HaloTag® pAb followed by secondary antibody labeled with Alexa Fluor® 633. The slide was scanned to detect the positive interactions (Figure 2, Panel A).
Panel A. Scanned image of the HaloLink™ custom array probed using prey proteins and the Anti-HaloTag® pAb. Panel B. Plot of the signal intensity obtained from HaloTag® Standard Protein (column 1 on the slide) and HaloTag® fusion bait proteins after probing with Anti-HaloTag® pAb (column 2 on the slide). Median fluorecent intensity is calculated from a 2.0mm diameter area.
Figure 2, Panel B, is the plot of median fluorescent intensity obtained from columns 1 and 2 of the scanned slide image and shows the relative amount of HaloTag® fusion proteins captured on the HaloLink™ slide. The binding of HaloTag® fusion protein to the HaloLink™ slide was specific because no signal was seen when the active site of HaloTag® protein is blocked by incubation with excess amount of HaloTag® Ligand (data not shown). The data shown with the HaloTag® Standard Protein in Figure 2, Panel B, were useful for normalizing the experimental variations resulting from: a) differences in expression levels of HaloTag® fusion proteins; b) differences in capture efficiency of individual HaloTag® fusion proteins; and c) intra- and inter-lot variability of HaloLink™ slides.
Figure 3 is the plot of fluorescent signal obtained when the HaloLink™ custom array (Figure 2, Panel A, columns 3–5) was probed with prey proteins. Positive interaction of c-Jun prey protein was seen with c-Jun, c-Fos, p53 and p65 bait protein. c-Fos interacted with c-Jun, p53 and p65 bait proteins, and p5o interacted with p53, p65 and β-galactosidase. These interactions have been previously reported in the literature(5)
, except for interaction between p50 and β-galactosidase.
Plot showing the fluorescent signal obtained from interaction of HaloTag® bait fusion proteins with prey proteins. Background (Bkg.) signal is the average of six wells that contained only cell-free lysate with no HaloTag® protein or PBSB. The standard deviation (δ) was calculated from these six wells. Bkg. + 3σ was used as the cutoff for positive protein:protein interaction.
We also tested the use of the HaloLink™ Protein Array Systems to study the modulation of protein:protein interactions by small molecules. We chose to study interaction between FK506 binding protein (FKBP) and the FKBP rapamycin binding domain of mTOR (FRB) proteins(7)
. Rapamycin is needed for FKBP and FRB to interact, and the protein:protein interaction can be modulated by changing rapamycin concentrations.
FRB was expressed as HaloTag® bait fusion protein in a TnT® SP6 High-Yield Wheat Germ Expression System and captured on HaloLink™ slides. Purified FKBP-GST (20μg/ml in PBSB) was added to the slides in the presence of varying amounts of rapamycin. The amount of FKBP-GST bound to FRB was detected using an anti-GST antibody and secondary antibody labeled with Alexa Fluor® 633. Figure 4 shows that FKBP binding is dependent on the concentration of rapamycin. No signal was measured in the absence of rapamycin.
The plot is shown on a log-log scale for clarity. Each point represents an average and standard deviation from triplicate experiments. Units are relative fluorescence units (RFU).
Using HaloTag®-PKA enzyme, we show that the 50-well format of the HaloLink™ Protein Array Systems also allows researchers to perform fluorescent enzyme assays. HaloTag®-PKA was expressed in TnT® SP6 High-Yield Wheat Germ Protein Expression System and captured on a HaloLink™ Slide in a 1-hour incubation. The slide was washed with PBSI containing 5% glycerol and spin-dried. The 5% glycerol in the wash buffer was needed to maintain enzyme activity during washing and drying(8)
. PKA activity was assayed using the ProFluor® Assay (Figure 5; see supplementary information for details). After the enzyme assay, the amount of PKA at the surface was determined using anti-PKA antibody. Results of the PKA activity assay correlated very well with the amount of PKA at the surface. With the ProFluor® Assay, the fluorescent signal has an inverse relationship with PKA activity, hence a lower amount of PKA at the surface will result in higher fluorescent signal. In addition to PKA, we have recently demonstrated the feasibility of performing other fluorescent enzyme assays on HaloLink™ slides(8)
HaloTag®-PKA fusion protein was expressed using TnT® SP6 High-Yield Wheat Germ Protein Expression Extract and serially diluted in PBSB. Five-microliter aliquots of each dilution were added in triplicate on a HaloLink™ Slide. The slide was incubated for 1 hour in a humidity chamber, washed with PBSB containing 5% glycerol and dried. The captured PKA was assayed using the ProFluor® PKA Assay (left Y axis). The amount of PKA on the HaloLink™ Slide was quantitated using an anti-PKA antibody (right Y axis).
A major concern for functional protein arrays is the stability of captured proteins. Our investigation(8)
identified that immobilized proteins may lose their functionality either during slide processing steps of washing and drying or during storage of printed slides. Using three enzymes (PKA, β-lactamase and β-galactosidase) fused with HaloTag® protein, we observed that adding 5% glycerol to wash buffers improved the stability of immobilized enzymes during slide processing steps. Surprisingly in the same study we found that washing and spin-drying had no significant impact on protein:protein interactions. All three enzymes immobilized on the HaloLink™ Slides retained their activity when stored at –20°C in 50% glycerol. However, storing the slide at –20°C occasionally resulted in the 50-well gasket coming off during subsequent spinning and drying steps.
To address the stability issue of printed proteins, we explored the 'print-on-demand' concept. All proteins needed for an array experiment may be expressed simultaneously in 96- or 384-well plates, stored at –70°C and used to print protein at the time of experiment. Our study showed no decrease in enzyme activity in the cell-free reaction mixes containing enzymes fused to the HaloTag® protein were exposed to multiple freeze-thaw cycles, captured on HaloLink™ slides and assayed for enzyme activity.
Using a single batch of proteins for printing proteins may eliminate problems with experimental data that result from batch-to-batch variation in cell-free expression systems. However we caution that it is indeed possible our freeze-thaw results may not be universally applicable, and optimization may be required for individual proteins.
In summary, we have shown that HaloLink™ Protein Array Systems can be used successfully to create custom protein arrays on hydrogel-coated surfaces. HaloTag®-fused proteins can be specifically captured from TnT® T7 Quick and SP6 High-Yield Wheat Germ Protein Expression Systems without any prior purification step, simplifying the protein array fabrication process. We show that HaloLink™ Slides allow multiplexed protein:protein interaction studies, screens for small molecule modulators of protein:protein interactions and fluorescent enzyme activity assays.
The authors thank the following HaloLink® Protein Arrays team members for their contributions: Brian Andersen, Carol Lindsay, Laurent Bernad, Mike Scurria, Patricia Bresnahan, Tanya Quint, Tenaya Noce, Paula Phenix, James Altwies, Michael Bayline and Ned Reimer.
Scientific Style and Format, 7th edition, 2006
Nath, N., Hurst, R., Hook, B., Schenborn, E., Meisenheimer, P., McDougall, M., Klaubert, D. and Storts, D.
Multiplexed Protein-Interaction Studies Using the HaloLink™ Protein Array System.
[Internet] 2009. [cited: year, month, date]. Available from:
American Medical Association, Manual of Style, 10th edition, 2007
Nath, N., Hurst, R., Hook, B., Schenborn, E., Meisenheimer, P., McDougall, M., Klaubert, D. and Storts, D.
Multiplexed Protein-Interaction Studies Using the HaloLink™ Protein Array System.
Promega Corporation Web site.
Updated 2009.
Accessed Month Day, Year.
Flexi, HaloTag, ProFluor and TnT are registered trademarks of Promega Corporation. HaloLink and Transcend are trademarks of Promega Corporation.
Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Products may be covered by pending or issued patents or may have certain limitations. Please visit our web site for more information.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.


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The vaginal yeast infection is every woman's nightmare. According to the U.S. Department of Health and Human Services' Office on Women's Health' three-quarters of all women will end up having at least one in their lifetime. Even worse' almost half will develop multiple yeast infections!
How do they happen? A type of fungus called candida albicans' commonly known as yeast' is normally present in the vagina. However' it can become too much of a good thing: an overgrowth of this healthy bacteria can result in an infection.
Itchiness is the most typical symptom of a yeast infection. Redness and swelling of the vagina and/or vulva' pain during urination or sexual intercourse' and a rash are also common. You should also watch out for an unscented' thick vaginal discharge that resembles cottage cheese.
Your first step before undergoing any course of treatment should always be to see a doctor and receive a diagnosis' especially if you're currently pregnant or have never been diagnosed with a yeast infection before. Make sure you don't have an STI beforehand.
If you have a yeast infection' there's absolutely nothing to be ashamed of. Still' it's no picnic; the irritation' itching' and burning in the vaginal area that result can be extremely painful. The discharge can make some women feel less confident in their daily lives. It can also temporarily interfere with your fertility if you're trying to conceive.
Over-the-counter medicines are often able to help treat yeast infections' even if they're recurring. Yeast Arrest is a natural suppository that provides a gentle' homeopathic course of treatment. It contains borax' hydrastis canadensis' berberis aquifolium' and kreosotum to help restore the vaginal pH balance. Customers say that the tea tree oil smells pleasant.
All you need to do is insert one suppository into the vagina in the morning' and another one at night. One of these packages should last for about two weeks' enough to treat one or more acute infections. You can also use a lower dose of Yeast Arrest suppositories for prevention.
Vitanica is committed to providing the best natural health supplements for women. It ensures that all of its products are made with organic herbs and extracts' and that they have been extensively tested for safety and effectiveness. Every Vitanica product is also vegetarian.
These statements have not been evaluated by the Food and Drug Administration (FDA). These products are not meant to diagnose' treat or cure any disease or medical condition. Please consult your doctor before starting any exercise or nutritional supplement program or before using these or any product during pregnancy or if you have a serious medical condition.
Each suppository includes: Active Ingredients as homeopathic: Borax 3X' Hydrastis Canadensis 3X' Berberis Aqufolium 3X' Kreosotum 10X
1 suppository morning and evening.
This product should be used after a health care provider has diagnosed a vaginal yeast infection.
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Overall Rating for Yeast Arrest - 28 Vaginal Suppositories
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By SPS
from Hoboken, NJ
Highly recommend!
Yeast Arrest by Vitanica works!
√ I would recommend this item to a friend.
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from erie, pa
Fastest delivery ever!
I love how fast my item was shipped!
I like that Yeast Arrest by Vitanica is a natural product.
√ I would recommend this item to a friend.
By Sarah Johnston
from Idaho
Life changing!!!
I have struggled with yeast infections for much of my life. My homeopath helped me get my hormones sorted out to prevent them, but whenever things get a little off and I end up with an infection - this fixes it! No more OTC meds for me!!! Last time I got back into a chronic yeast infection cycle I used Yeast Arrest 28 Vaginal Suppositories by Vitanica along with Arden's Powder rinses to break the cycle. Can't recommend this product enough!
Cures yeast infections!!
√ I would recommend this item to a friend.
By J
from Los Angeles, CA
Effective relief and prevention
I have health issues that cause chronic candidiasis. I need a multi-pronged approach. This product is one of my essentials. When candida gets out of control I need more help but Yeast Arrest allows me to prevent that and definitely gives symptom relief all the time.
Effective prevention
Quick relief
√ I would recommend this item to a friend.
By Hope
from San Diego
Yeast Arrest Works!
Finally a product that works. After three years of Metro Gel plus countless hours of suffering I finally found a product that has taken me out of discomfort and pain. And the best part it is homeopathic and I do not need a doctors prescription. I would recommend Yeast Arrest to every female. I cant thank the makers of Yeast Arrest enough for giving me back the quality of my life. Great for yeast and bv. Follow the instructions and keep refrigerated.
Extremely effective when used as directed. Affordable.
Homeopathic. No more doctor visits. You can feel the improvement within 24 hours.
You may have to use for several weeks or months if you have had repeated infections. Messy if not stored in the refrigerator.
√ I would recommend this item to a friend.
By Angela
from DFW, Texas
So Far: Short Term Works! Wishes there w
Had a ridiculously long yeast infection - it would get better come back go away come back. SO annoying and I refused to see the doctor since Ive heard horror stories about how traditional biomedicine for a womans vagina does more harm than good - so I stuck with natural methods (garlic tea tree oil yogurt etc.) and though I stuck with it for a while the lifestyle changes proved way too hard for me to maintain so the yeast infection kept coming back. I ended up buying an OTC popular brand one day when I just couldnt take it anymore. It worked but the infection came back after two weeks and a vacation in water/wet swimsuits for several days straight. I finally started Yeast Arrest and I immediately felt better! I just wished several things. A) Delivery of this product NEEDS to come in a packed cool box or something. If the product melts IN YOUR HAND can you imagine how it traveled to Texas in July? It melted and I immediately placed in the fridge where it reshaped itself but with large bubbles near the front end of the suppository. B) Applicator. I really wish there was a simple applicator in insure I could push up the suppository as far as it can go my muscles are pretty strong and kept forcing it out even when relaxing. Im considering using a tampon tonight via cutting off the end etc. C) Perhaps come in a stronger dosage of ingredients? I used one and the next day it felt better but still itchy. So the next night I inserted two and I feel so much better. Have done three nights of two suppositories and on this fourth day Ive had less discharge and less itchy symptoms.
Smells Great
No Side Effects (major)
Works Immediately to Soothe
No Applicator for better placement
Easily melts in hand so insert fast!
Slight burning but more of a soothing burn
√ I would recommend this item to a friend.
By River
from San Diego
Yeast Arrest is the only item that has helped me not have constant yeast infections. I was on diflucan continually for over 6 months to treat chronic yeast infections. The doctors including specialists had no idea why it would not disappear. Looking back I believe that my hormones changed after the birth of my first child. I can use this to prevent the yeast infections rather than waiting for them to occur (which was often) and then treating them with the over the counter creams which took awhile to take effect only to have to retreat when the reoccurred. I found Yeast Arrest a couple of years ago and am grateful to have this under control now! Please read the instructions as it indicates that you are supposed to use this every night including during your menses to prevent yeast infections.
√ I would recommend this item to a friend.
By Rachel
from Philadelphia, pa
Like many of the reviews have stated I was struggling with yeast infections for a while not even full blown horrible kinds but just enough to make life uncomfortable. I tried everything was using garlic at night and apple cider vinegar soaks during the day but it just didnt cut it. Now I keep these on hand at the slightest feeling of it returning I put one in at night for 2 nights. Thats one of the things I like best about these you can nip it in the bud clean and easy. I seriously have never written a review online before but I think Yeast Arrest is an awesome product and so important for women to be able to heal themselves and not put these horrible things in their bodies that doctors try to give us. Anyway Yeast Arrest was recommending by a friend who was severely suffering from BV and she said this saved her as well. Good luck ladies!
√ I would recommend this item to a friend.
By Ann
from San Francisco, CA
Really helpful for yeast infection
After trying every natural way of healing a stubborn yeast infection (garlic yogurt ACV you name it!) I stumbled upon Yeast Arrest. It definitely helped heal my condition along with a yeast free diet regiment. Now I use Yeast Arrest regularly during/after menses and sex. It is really a great preventer and helps maintain my PH.
√ I would recommend this item to a friend.
By Jane
from Groveland, MA
Yeast Arrest
Daughters Naturopath Dr. suggested Yeast Arrest and they work great.
√ I would recommend this item to a friend.
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Root's hydroponic system helps with water, nutrients and light.
ZDNet and CNET writer Zack Whittaker is in the studio today talking Internet security, attention spans, the Light Phone and what "words" have been added to the Scrabble dictionary.
The Groundfridge is an easy-to-install natural refrigerator. You just need a hole in the ground.
An upswell of acquisitions and shifts in company culture are changing how Silicon Valley looks at design. And that's reshaping the tech industry, one of the leading design experts says at SXSW.
The Hamilton Beach 33642 Programmable Right Size Multi-Quart Slow Cooker features the ability to accommodate gatherings large and small. Graduated markings inside the crock designate serving size.
The Kalamazoo Outdoor Gourmet Built-in Artisan Fire Pizza Oven offers an elegant way to bake pizza right from the comfort of your backyard.
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The fast track to all-mountain dominance.
Want a faster way to get in and out of the world's most popular snowboard boot? The Thirty Two Women's Lashed FT Snowboard Boot has a quick lace system to help you spend less time in the parking lot and more time on the slopes. Not only does the Fast Track lacing system make getting in and out of your boot quick and easy, it also allows you to individually adjust four different zones on the boot so you can dial in the perfect fit for your foot every time.
The Level 2 Intuition liner is heat-moldable for a custom fit, and a molded EVA insole cushions your feet for all-day comfort. Plush, the liner is capped off with a soft Sherpa fleece cuff for cozy comfort. High-density Evolution Foam in the outsole also improves cushioning, and it's much lighter than rubber to reduce weight without sacrificing grip so you can still bootpack up icy slopes and rocky ridges. And with a medium flex that allows you to conquer everything from steep tree runs to urban rails, the Lashed FT is truly a tough boot to beat.
- Fast Track lacing
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From Jackie Kennedy and Sophia Loren to Elizabeth Taylor, everyone has a favorite '60s fashion icon. How can you not love the era's full skirts, cinched waists, and, of course, the pearls? The Affinity Dress by Eva Franco features a fitted shape with a flattering, mini-flare skirt, making it a modern rendition of the wiggle dress.
Size 0: Bust 32.5". Waist 24". High Hip 29.5". Low Hip 35.5".
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From flea market purveyor to having 800 stockists worldwide, Eva Franco has come a long way in fashion. The Romanian-born designer used her passion to connect with American peers when she emigrated to the U.S. After graduating from the Fashion Institute of Technology, she launched her own line in LA, where she produces elegant pieces that celebrate the female form.
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text: NARS Desire Blush ($28.00 for 0.16 oz.) is described as a "delicate bright pink." It's a vibrant blue-based magenta pink with a mostly matte finish. MAC Peony Petal is lighter, more bubblegum pink. MAC Florida is very similar, perhaps a little less pink. Tarte Amused is pinker. MAC Amazon Princess is not as vibrant and more purple. Make Up For Ever #3 is pinker, darker.Read Full Review


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Created under the umbrella of Hidden Moves—a professional moniker representing the Welsh artist and designer Rhys Owens—this print is inspired by its creator's childhood penchant for Rubik's cubes, ThunderCats, and the ZX spectrum. Owens's singular aesthetic, which combines photography, illustration, and digital imaging, has inspired an equally special cast of surreal characters who inhabit the Hidden Moves universe. Collect them all!
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Sweetwater's junior varsi-ty girls will be 21-0 when they go to Monahans today.
Sweetwater (7-0 in dis-trict) needs three more wins to have a perfect season after rolling to a 47-5 win at Greenwood Friday. Shaneese Brown and Abby McPherson had 10 points while Cierra Knox and Morgan Whitley had eight, Maddie Jones five, Jasmine Reddic four and Jaya Adkins and Nisha Williams one. Sweetwater led 29-1 at halftime and 40-3 after three periods of play.
The freshman girls lost 30-13 in their contest and are now 4-3 in district and 11-7 overall. No individual scoring totals were reported for the freshman game.
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Dublin is a big city with a small-town mentality. It's a city where famous writers and drinkers cavorted (and brawled) in many of the same bars and where it's not unusual for a local to strike up a conversation with a stranger in a pub. Today, many of the most popular attractions revolve around the city's rich literary and drinking history. In the literary category, there's the Book of Kells, the Old Library at Trinity College, The Oscar Wilde House, and the Dublin Writer's Museum; in the drinking category, there is the Guiness Storehouse, the Old Jameson Distillery, and numerous historic pubs. The Literary Pub Crawl relates to both, with spirited literary-themed performances and visits to some of the favorite bars of Dublin literati such as James Joyce and Brendan Behan.
Dublin's nightlife scene has been hopping for decades -- or centuries, rather -- and it's still thriving today. University students flock to the rowdy Temple Bar area, while the bars near St. Stephen's Green tend to draw a classier set. The city center is very dense, so traveling by foot is easier here than in many other cities in Europe. Though the Luas train system is not well-used, there is a fairly reliable bus system and a pay-as-you-go bicycle scheme has added to the ease of getting around.
One of Dublin's strongest assets is its compact city center. This means that regardless of where you stay, reaching another neighborhood downtown will require minimal effort, so it simply becomes a matter of preference. Temple Bar is the city's nightlife mecca, and is home to everything from quaint B&Bs to completely divey hostel-style inns. Be warned: This is a convenient area for people looking to barhop, shop, or dine out, but it's known to be noisy into the wee hours.
St. Stephen's Green draws a more upscale crowd, and visitors will find charming historic pubs and excellent shopping along Grafton Street. Some hotel rooms here have park views.
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This story was updated at 8:20 a.m. Aug. 7, 2012.
Prince George's County Police identified the 18-year-old who was fatally shot early Monday morning in an alleged botched robbery in Temple Hills and the suspected shooter.
Police said just after midnight in the 5000 block of Temple Hill Road, Darrel Sales, 18, of the 6100 block of Kildare Court in Fort Washington, attempted to rob two victims when a fight broke out, leaving Anthony Boatwright of the 5800 block of Foote Street NE, Washington, D.C., fatally wounded from a gunshot.
"The suspect grabbed a gun from his waistband and shot one of the victims," said Sgt. Sonya Rorls, a county police spokeswoman.
Rorls said officers were nearby when the incident took place, and were able to locate and apprehend Sales minutes later. Boatwright was pronounced dead on the scene, police said.
"The homicide unit was able to develop a suspect right away because one of the victims on scene was able to identify the shooter."
A number was listed for Boatwright's address but was not working on Tuesday. A number could not be immediately located for Sales on Tuesday and no attorney information was listed for him in online Prince George's County District Court records.
The other victims in the attempted robbery was uninjured, Rorls said.
Sales is charged with second-degree murder, use of a firearm in a felony crime and first degree assault.


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Limp Bizkit NOT Breaking Up, Wes Borland Insists Via Twitter
Thought you'd seen the last of Limp Bizkit? Think again. In a Twitter post yesterday, guitarist Wes Borland denied that the seminal nu-metal outfit is breaking up, despite what singer Fred Durst implied in a recent interview.
"LB has not called


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Kuhlmann Makes USA CONCACAF Roster
Civana Kuhlman has been called up to the all-important U17 World Cup Qualifying campaign. Next to the World Cup itself, there is no more important event for a National Team. The U17 team is often considered the most important youth team and this marks the third straight qualifying that a CO Rush player has been called into the team. What is even more unique for this one is that Civana ("C") is age eligible for the U15 National Team; she's playing two years up for our country!
C is in great company as she follows in the lines of previous youth national team players: Sarah Wagenfuher, Jordan Angeli, Morgan Stanton, Lindsey Horan, Gabbi Miranda, Lizzy Raben. The tradition continues! It continues because of the Rush Way and because of the excellent teammates each of these great young players have played alongside, coupled with the talent and determination to push forward to the highest levels our country has to offer.
Additionally, if not for injury Sabrina Macias had solidified herself as a U17 National Team member and we'll look forward to her healthy return in a handful of months. And, pointing to the depth of the club, Betsy Brandon, Jordan DiBiasi and Rebecca Rasmussen have each been called into the next U18 National Team camp.
Congratulations girls! And, all the very best to Civana as she represents our club and our country!
For the full roster please click here.


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Elder Kaeden Kulow will be returning from serving a mission for the Church of Jesus Christ of Latter-day Saints in the Texas Lubbock Mission on Wednesday, Sept. 5. He will be speaking on Sunday, Sept. 9 at 11 a.m. at the Price Third Ward. Elder Kulow is the son of Cory and Tawnya Kulow and the grandson of LeDonna Hall and the late Chester Hall and Larry and Janice Kulow.
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