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Description:HP StorageWorks MSA60 Modular Smart Array base enclosurePart Number(s)Manufacturer Part# 418408-B21
Overview:The HP 60 Modular Smart Array enclosure is a 2U Serial Attach SCSI (SAS) disk drive storage enclosure supporting 3.5 inch SAS Serial ATA (SATA) and SAS Midline (MDL) drives. This enclosure delivers industry-leading data performance availability storage density and upgradeability to meet customers' demanding and growing storage needs.
Sub-CategorySmart Array
Part Number418408-B21
Products ID457973
Product TypeModular Smart Array
LED IndicatorsHDDs, Front, Rear
Host Interface4 x wide SAS 3 Gb/s ports
Max. Number of Drives12 @ 3.5 in SAS/SATA
Operating Temperature50° to 95° F (10° to 35° C)
Rated Input Voltage100 to 240 VAC
Rated Input Frequency50 to 60 Hz
Input Power (max)345 W
RAID Support0, 1, 1+0
UpgradeableYes, Online Only
Drive & Enclosure Support3.5 inch SAS 3 Gb/s
Number of Ports2
Form Factor2U Rack
Dimensions (WxHxD)17.6 x 3.5 x 23.3 in (44.8x8.8x59 cm)
[ Manufacturers Specs ]


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Welcome to June! I can't believe 2014 is almost halfway over. The weather continues to warm up, school is going on break or is already finished, and vacations are underway. Here are some great deals to start your June off right.
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Stock Yards
Now is the perfect time to order premium meats for your upcoming BBQ or get dad something for Father's Day. Get top cuts of meats at great prices at Stock Yards. They supply their meats to some of the best steakhouses in the country, so you know you're getting the prime cuts. If you order $75+ worth of food, you can get 12 free USDA steak burgers with code BURGERJN. Expires 6/30
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Still looking to get into shape for your beach body? It's not too late to start working out and stock up on great running and workout gear. One of my favorite running sites is Road Runner Sports. They have everything I need from shoes to clothing and accessories. Get 10% off and free shipping when you use code COUPONSRUN14. Expires 6/14
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subset: commoncrawl


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Friday, September 15, 2006
At Last, the Issue is Publicly Joined . . . and When All the Smoke has Cleared, the Central Question is Quite Simple
Marty Lederman
And it is this: Should the CIA be legally authorized to breach the Geneva Conventions by engaging in the following forms of "cruel treatment" prohibited by "common" Article 3(1)(a) of those Conventions?:
Good post Marty.
My analysis is that Congress has no authority to authorize violations of Geneva in the first place.
(1) Each of the four Geneva conventions contains a clause on denunciations, which states:
"Each of the High Contracting Parties shall be at liberty to denounce the present Convention.
"The denunciation shall be notified in writing to the Swiss Federal Council, which shall transmit it to the Governments of all the High Contracting Parties.
"The denunciation shall take effect one year after the notification thereof has been made to the Swiss Federal Council. However, a denunciation of which notification has been made at a time when the denouncing Power is involved in a conflict shall not take effect until peace has been concluded, and until after operations connected with the release and repatriation of the persons protected by the present Convention have been terminated.
"The denunciation shall have effect only in respect of the denouncing Power. It shall in no way impair the obligations which the Parties to the conflict shall remain bound to fulfil by virtue of the principles of the law of nations, as they result from the usages established among civilized peoples, from the laws of humanity and the dictates of the public conscience." GC III art. 142, GC IV art. 158.
Under the supremacy clause, that is US law, and the principle that one does not change or suspend the rules during an armed conflict is nothing new. The very first US treaty that touched on this was the 1785 Treaty of Amity and Commerce with Prussia, which states:
"[I]t is declared, that neither the pretence that war dissolves all treaties, nor any other whatever, shall be considered as annulling or suspending this & the next preceding article, but on the contrary, that the state of war is precisely that for which they are provided, & during which they are to be as sacredly observed as the most acknowledged articles in the law of nature or nations." Id. art. 24.
(2) Even if you make the Geneva conventions vanish, what remains are the Nuremberg Principles and customary law going back to Hague 1907, the Lieber Code (1863), and beyond.
The Bush administration's war crimes will still be crimes against humanity, and any effort by Congress to provide them cover for those crimes will also be a crime.
What people need to understand is that no terrorist is as a great a threat to us as a government that will not obey our own laws. Bush and Cheney are criminals, and that is all that they are.
"The question is not simply whether, in the abstract, it would be a good or acceptable idea for the United States to use such techniques in certain extreme circumstances on certain detainees. ... [T]hat is obviously a question on which there is substantial public disagreement, much of it quite sincere and serious." Really? Perhaps it is a serious question if we limit ourselves to a "ticking bomb" hypothetical. The language "certain extreme circumstances on certain detainees" amounts to a "ticking bomb" hypothetical, because, in reality, if torture is allowed, it will be used indiscriminately, as it has been. In the real world, whether torture is good does not seem to be a question upon which reasonable people differ. There seems to be unanimity among reasonable people that torture produces no valid intelligence and serves no purpose except putting our soldiers at risk. Bush and Cheney's contrary view can be explained only by their particular psychological needs.
So, if we were to use techniques like those used against the mastermind of the 9/11 attack -- techniques that even Graham and McCain acknowledge allowed the U.S. to stop additional attacks inside the U.S. -- we should not use them because they could be construed as being in violation of of the Geneva convention.
I think the appropriate phrase is that our Constitution is not a suicide pact. The Constitution trumps the Geneva Convention so I'll go with it.
If we use techinques that have proven successful in stopping attacks, we should use them.
It is a false premise that doing so will ut our soldiers in greater danger. When the militant Islamists capture our soldiers they behead them -- they certainly do not follow the Geneva Convention.
jdri - I suggest that you read this post from an actual solider on this subject - http://time.blogs.com/daily_dish/2006/09/what_weve_lost.html
In any case the actions of the terrorist should have no impact on our actions. Have we come so far from that "Shining city upon a hill"?
President Bush repeatedly asserted today at his press conference that the problem is merely one of ambiguity, that all the CIA is seeking is clarity about what Common Article 3 prohibits. (At the same time, he and his administration have refused to say specifically what interrogation techniques "the program" has employed.)
As I understand it, DOD just clarified with specificity in the new field manual what is prohibited. Why cannot the War Crimes Act simply reference that same specificity?
Isn't it because Bush, Hayden and other executives know -- but are afraid to say out loud -- that the CIA techniques unambiguously violate those specific standards?
It is not simply a matter of whether militant Islamists behead American POWs. The following, pasted from Andrew Sullivan's blog, is from a marine describing his experience in Desert Storm:
If those men had even put up token resistance, some of us would not have come back. But they didn't even bother, and surrendered at least in part because of our reputation. Our two hundred year old reputation for being fair and humane and decent. All the way back to George Washington, and from President George H.W. Bush all the way down to a lance-corporal jarhead at the front.
Its gone now, even from me. I can't get past that image of the Iraqi, in the hood with the wires and I'm not what you'd call a sensitive type. You know the picture. And now we have a total bust-out in the White House, and a bunch of rubber-stamps in the House, trying to make it so that half-drowning people isn't torture. That hypothermia isn't torture. That degradation isn't torture. We don't have that reputation for fairness anymore. Just the opposite, I think. And the next real enemy we face will fight like only the cornered and desperate fight. How many Marines' lives will be lost in the war ahead just because of this asshole who never once risked anything for this country?
I think the appropriate phrase is that our Constitution is not a suicide pact. The Constitution trumps the Geneva Convention so I'll go with it.
"This Constitution, and the Laws of the United States which shall be made in Pursuance thereof; and all Treaties made, or which shall be made, under the Authority of the United States, shall be the supreme Law of the Land; and the Judges in every State shall be bound thereby, any Thing in the Constitution or Laws of any state to the Contrary notwithstanding." -- Article VI
For all the talk of suicide pacts, there appear to be people who don't mind torturing the Constitution to death.
Although the exact details of this program might change, more important is the arguments used by George Bush to push it through Congress: that these harsh techniques will prevent future attacks.
Think about this: we are not safe from 20 guys with box cutters!
What attack is Bush talking about? Another 9/11?
If our security relies upon us catching a terrorist who is planning an attack or knows the details of an attack, we should all be very afraid. Because what limit is there? This belief that we need to catch and torture people to ensure we don't get attacked again means that nobody is safe.
The argument is essentially that our government can do anything if they think it might prevent an attack.
During the Second World War in Europe, at its very end, the Wehrmacht was far more likely end resistance before the British, French, and American forces, which had a reasonable reputation for humanity, than before the Soviets, who had raped and murdered from East Prussia and Pomeriania on. This fact saved American lives.
And, really, if these techniques are all that effective and efficient, why not use them for everything? Homicide investigations, child molestation investigations, down even to that, in any Republican's eyes, most forgiveable of crimes: tax evasion. I'm surprised at Bush's reticence. What a piker.
Tom _i: You don't need to refute Bush's arguments, because they are not worthy of being called "arguments" and are probably not even intended to be arguments. They are just fear-mongering.
Bush people appear dead scared of being held legally responsible for they misdeeds once a new Congress and administration take charge of things, and this appears to be the primary motivation for almost everything they do these days.
Evidence for it is everywhere.
Take Specter for example - it is now quite clear that his primary goal was to protect Bush from legal consequences of his violations of surveillance statutes. Everything else in his bills is either a smoke screen or legal padding to hide his true intentions. And they are obviously quite desperate about it as witnessed by Spoecter's reckless willingness to take the country back (via his bills) to pre 1978, Hooverian days or by his public lies about including explicit blanket amnesty for Bush in his bill.
The current discussion likely has a similar origin. It seems CIA concluded that they could be facing open courts ("War Crimes Act") once Bush is gone, so they went to their old boss papa Bush who out of sense of loyalty committed himself to get Junior to do something about it. And as seen yesterday Junior accepted the challenge wholeheartedly. "Partners in crime" so to speak.
The need to shield the CIA for their deeds not the future "effectiveness" of CIA interrogators is what is really driving Bush here.
There is another aspect to all this apart from international fallout if Congress accedes to Bush's demands and that is why on earth only CIA should be permitted such techniques?
FBI after all is as good an agency as CIA and they may need to resort to "alternative" techniques when interrogating serious domestic "terror" suspects too. Think Mayfield or some eco-terrorists for example.
Same thing applies to local police, being able to "force a prisoner to stand naked in a cell kept near 50 degrees, during which he/she is doused with cold water" would do wonders to the effectiveness of local law enforcement agencies in serious domestic cases.
And finally, as Lederman I see no connection here to Geneva's prohibition on "outrages against human dignity," and "humiliating and degrading treatment."Post a Comment
Let us be honest, if we really considered ourselves bound by Geneva as far as human dignity is concerned we would have to close down almost all of our prisons and, sadly, defrock quite a number of judges out there.
Books by Balkinization Bloggers
Stephen M. Griffin, Broken Trust: Dysfunctional Government and Constitutional Reform (University Press of Kansas, 2015)
Frank Pasquale, The Black Box Society: The Secret Algorithms That Control Money and Information (Harvard University Press, 2015)
Bruce Ackerman, We the People, Volume 3: The Civil Rights Revolution (Harvard University Press, 2014)
Balkinization Symposium on We the People, Volume 3: The Civil Rights Revolution
Joseph Fishkin, Bottlenecks: A New Theory of Equal Opportunity (Oxford University Press, 2014)
Mark A. Graber, A New Introduction to American Constitutionalism (Oxford University Press, 2013)
John Mikhail, Elements of Moral Cognition: Rawls' Linguistic Analogy and the Cognitive Science of Moral and Legal Judgment (Cambridge University Press, 2013)
Gerard N. Magliocca, American Founding Son: John Bingham and the Invention of the Fourteenth Amendment (New York University Press, 2013)
Stephen M. Griffin, Long Wars and the Constitution (Harvard University Press, 2013)
Andrew Koppelman, The Tough Luck Constitution and the Assault on Health Care Reform (Oxford University Press, 2013)
James E. Fleming and Linda C. McClain, Ordered Liberty: Rights, Responsibilities, and Virtues (Harvard University Press, 2013)
Balkinization Symposium on Ordered Liberty: Rights, Responsibilities, and Virtues
Andrew Koppelman, Defending American Religious Neutrality (Harvard University Press, 2013)
Brian Z. Tamanaha, Failing Law Schools (University of Chicago Press, 2012)
Sanford Levinson, Framed: America's 51 Constitutions and the Crisis of Governance (Oxford University Press, 2012)
Linda C. McClain and Joanna L. Grossman, Gender Equality: Dimensions of Women's Equal Citizenship (Cambridge University Press, 2012)
Mary Dudziak, War Time: An Idea, Its History, Its Consequences (Oxford University Press, 2012)
Jack M. Balkin, Living Originalism (Harvard University Press, 2011)
Jason Mazzone, Copyfraud and Other Abuses of Intellectual Property Law (Stanford University Press, 2011)
Richard W. Garnett and Andrew Koppelman, First Amendment Stories, (Foundation Press 2011)
Jack M. Balkin, Constitutional Redemption: Political Faith in an Unjust World (Harvard University Press, 2011)
Gerard Magliocca, The Tragedy of William Jennings Bryan: Constitutional Law and the Politics of Backlash (Yale University Press, 2011)
Bernard Harcourt, The Illusion of Free Markets: Punishment and the Myth of Natural Order (Harvard University Press, 2010)
Bruce Ackerman, The Decline and Fall of the American Republic (Harvard University Press, 2010)
Balkinization Symposium on The Decline and Fall of the American Republic
Ian Ayres. Carrots and Sticks: Unlock the Power of Incentives to Get Things Done (Bantam Books, 2010)
Mark Tushnet, Why the Constitution Matters (Yale University Press 2010)
Ian Ayres and Barry Nalebuff: Lifecycle Investing: A New, Safe, and Audacious Way to Improve the Performance of Your Retirement Portfolio (Basic Books, 2010)
Jack M. Balkin, The Laws of Change: I Ching and the Philosophy of Life (2d Edition, Sybil Creek Press 2009)
Brian Z. Tamanaha, Beyond the Formalist-Realist Divide: The Role of Politics in Judging (Princeton University Press 2009)
Andrew Koppelman and Tobias Barrington Wolff, A Right to Discriminate?: How the Case of Boy Scouts of America v. James Dale Warped the Law of Free Association (Yale University Press 2009)
Jack M. Balkin and Reva B. Siegel, The Constitution in 2020 (Oxford University Press 2009)
Heather K. Gerken, The Democracy Index: Why Our Election System Is Failing and How to Fix It (Princeton University Press 2009)
Mary Dudziak, Exporting American Dreams: Thurgood Marshall's African Journey (Oxford University Press 2008)
David Luban, Legal Ethics and Human Dignity (Cambridge Univ. Press 2007)
Ian Ayres, Super Crunchers: Why Thinking-By-Numbers is the New Way to be Smart (Bantam 2007)
Jack M. Balkin, James Grimmelmann, Eddan Katz, Nimrod Kozlovski, Shlomit Wagman and Tal Zarsky, eds., Cybercrime: Digital Cops in a Networked Environment (N.Y.U. Press 2007)
Jack M. Balkin and Beth Simone Noveck, The State of Play: Law, Games, and Virtual Worlds (N.Y.U. Press 2006)
Andrew Koppelman, Same Sex, Different States: When Same-Sex Marriages Cross State Lines (Yale University Press 2006)
Brian Tamanaha, Law as a Means to an End (Cambridge University Press 2006)
Sanford Levinson, Our Undemocratic Constitution (Oxford University Press 2006)
Mark Graber, Dred Scott and the Problem of Constitutional Evil (Cambridge University Press 2006)
Jack M. Balkin, ed., What Roe v. Wade Should Have Said (N.Y.U. Press 2005)
Sanford Levinson, ed., Torture: A Collection (Oxford University Press 2004)
Balkin.com homepage
Cultural Software
The Information Society Project
Useful Links
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For first-year Bowie High School tennis coach Michael Fehn, the Simmons sisters, Torri and Maddi, are virtually interchangeable.
The identical twins — Torri's older than Maddi by two minutes — have alternated playing first and second singles throughout the season because their skill sets are versatile enough to keep the lineup fluid, Fehn said.
"Usually I can't tell them apart," Fehn said with a laugh. "That's why I said they're interchangeable. People on the team, because they've known them, can tell them apart, but I'm still sometimes like, 'Which one are you?'"
It's a fairly common occurrence for the Simmons sisters, who started playing tennis seriously in the seventh grade.
"He [mixes us up] about every day. It's kind of hard for him," Maddi said. "It's hard for everyone, but it's also his first year, so we'll give him a pass. Next year, though."
So far this season, the only loss by either of the sisters came when Torri fell to Eleanor Roosevelt's Lydia Carter in the only match the Bulldogs lost as a team. Other than that, whether playing first or second singles, Torri and Maddi have been the steady presence Bowie has needed to turn in a standout season.
"If I ask them to help out another kid, tutor them or hit balls during practice, they're really helpful about it," Fehn said. "They have great sportsmanship and if I ask them to sit once or twice so other teammates can play, they're fine with it. They're really team players."
While the pair has spent the majority of Bowie's regular season playing singles, they're leaning heavily toward playing together as a doubles team once the postseason tournaments roll around.
"It has benefited both of us both greatly," Maddi said of alternating first and second singles. "It's a good idea to switch it up. It gives us different perspective on the various players throughout the county."
Last year, they played doubles together and won the district and placed third at regionals. This season, the pair of juniors are hopeful that they'll be able to reach the state tournament.
"It has its pros and cons," Torri said of playing doubles with her identical twin. "It's a little bit harder because there's so much off-court interaction that we have to make sure we really stay focused on the court. But I also think it's really intimidating for our opponents."
Maddi agreed that the switch from singles to doubles at season's end doesn't seem as daunting a move as many would think.
"We played in a league during the offseason and had some matches together and we've always practiced doubles," Maddi said. "It's different because I know her. I know her potential. If I'm playing with someone new, or that I don't know, it's harder to know their style. I think I play a more passive style. I try to keep it consistent and it works for me. She's more aggressive and that works well for her."
After attending a local swim club to swim, the sisters noticed the tennis courts at the facility and figured they'd give the sport a try. They haven't looked back since.
"The twins are great," Fehn said. "Their parents are really helpful, too. They donated the wind screens we have at Bowie. They're very involved with their kids and they've done a great job. They've got them on track and they've really helped them to be excellent."


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text: Action - Sisters Bonnie and Debbie are visiting the zoo when 16-year-old Debbie is drugged and kidnapped. Bonnie witnesses the event, but is unable to stop it. When Bonnie goes to the police, she learns that Debbie has been taken to a mansion owned by a notorious child pornographer. Meanwhile, the kidnappers set out to find and kill Bonnie, thus eliminating their only witness. Undaunted, Bonnie teams up with an undercover police officer and infiltrates the seedy underworld of child pornography to rescue her sister.


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|News » Archives
Recap | BoxscoreBucs pick up fifth straight win against Mercer, 2-1
Sunday, October 03, 2010
JOHNSON CITY, Tenn. (Oct. 2, 2010) - The ETSU men's soccer team picked up its fifth straight win as the Bucs netted two goals in the second half against Mercer to come from behind 2-1 Saturday night at Summers-Taylor Stadium.
"This was a very physical contest between two teams with contrasting styles," said head coach Scott Calabrese. "More importantly, it was a crucial conference match to keep ourselves atop the Atlantic Sun standings. I am extremely proud of the way we conducted ourselves tonight."
The Bucs (7-2-0, 2-0-0 A-Sun) dominated the game offensively as they outshot Mercer (5-2-1, 1-1-0 A-Sun) 20-5 in the contest putting eight shots on frame, while the Bears had a slight edge in corners, 3-2. Sophomore Ronnie Hreha (Asheville, N.C.) had to effort just one save in the contest as he improved to 4-1 on the season.
"There were several occasions where the game could have gotten out of control, but we were able to maintain our composure and fight back from a one-goal deficit to win the match," Calabrese said. "The players deserve tremendous credit as they showed they are determined to overcome difficult situations, to work hard and to win."
Freshman David Geno (Kingsport, Tenn.) looked to have put the Bucs on the board in the 24th minute as he blasted a wide open shot from inside the penalty area, but MU's Brett Petricek was up to the challenge as he made a nice save to keep the game scoreless.
Mercer jumped out in front 1-0 in the 36th minute as Ian Cameron sent a corner kick into the side of the net past an ETSU defender for a goal.
In the second half, the Buccaneers evened the match at 1-all on a goal by Alesi Osorio (Austin, Texas) in the 53rd minute. The Bucs drew a foul deep on the Mercer end of the field, and Osorio was in the right spot to knock in the rebound from the free kick by senior Guilherme Reis (Belo Horizonte, Brazil).
Osorio then came up with a big save in the 60th minute as Taylor Krek fired a shot towards the open end of the ETSU net, but Osorio was there to keep the ball from going in as he headed it away from danger.
ETSU then went in front in the 78th minute as Geno fired a shot on goal that was saved by Petricek, but freshman Blaike Woodruff (Georgetown, Texas) beat a Mercer defender and slotted the rebound from the shot into the back of the net to give ETSU a 2-1 advantage.
The Buccaneer defense played strong down the stretch as they did not allow a shot by the Bears in the final 29 minutes of the contest.
The Bucs are back in action next Friday at 7 p.m. as they head to DeLand, Fla. to take Stetson. For more information on ETSU men's soccer and all other Buccaneer athletic news please visit www.ETSUBucs.com.


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. Photograph 1966 by George P. Landow. [You may use this image without prior permission for any scholarly or educational purpose as long as you (1) credit the photographer and (2) link your document to this URL.]
Last modified 5 January 2006
Victorian Web Home —> Places —> The Lake District —> Next]


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Acquia Comments on EU Recommendations for Data Processing in the Cloud
On July 1, 2012, European Union (EU) data protection regulators provided guidelines for service providers processing personal data in the cloud for client organizations. The opinion describes associated risks and provides recommendations for best practices. This brief white paper explains why Acquia Cloud is aligned with the guidance provided by the EU data protection regulators ensuring that the transfer of EU personal data to cloud service providers such as Acquia may take place lawfully under EU law.


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I'm eating it. Still rooting for the kid though. Not like he needed anymore motivation...
I'm eating it. Still rooting for the kid though. Not like he needed anymore motivation...
Diehard Fan Living in Packer Territory
Co-Founder: 2 Mugs Fantasy Football -- a site about and football!
I enjoy MyMan's post. I don't see why we can't question Alex smith. Season starts, pretty sure everyone that's questioning him won't be rooting against him. I dis liked Cassel but i wasn't hoping he continue to suck. Alex has shown better stats the past 2 years but at the same time he has those first years that were bad. Doesn't matter what happens, he sucks his first game, someone will make a post of I told you so, he does good we'll see a post that says I told you so.
Also this really remind me when Tyler Thigpen came in and people were trying to make a case that he should come back and be our starter because he did better then Big Ben.......i have to admit even those we only won like 2-4 games that year, i had fun but that was probably because i was a bar with a lot of friends
saw it in another thread (was posted because they were talking about tebow gifs that were posted)
|Thumbs Up
|Received: 50
Given: 3
Peyton's INT against Baltimore was worse than throw.
For those of you that have had enough of MyManHali, you can easily ignore him by clicking this link.
Click here to ignore posts from MyManHali


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I believe any man's finest hour - his greatest fulfillment to all he holds dear, is that moment when he has worked his heart out in a good cause and lies exhausted on the field of battle...Victorious
feel your pain bro. This effort of late has really exposed this team. We arent very good at any particular position except punting right now. Guys are either average in skill and talent and effort or below avg. This really falls back to Ireland imo. I dont understand how yo u dismantle a team from a certain regime to a new era in offense and defense and coaching and u keep the same gm from the previous regime, your not really changing much. your still gonna get the same type of players running a difference scheme.. we need better players period man. We need a stand out guy at every position to do anything now a days.


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CLARION – It didn't take long for Johnsonburg to assert its will over Eisenhower.
After an emphatic opening block from Cameron Grumley on the game's first sequence, the Rams sprinted to a 17-7 first-quarter lead en route to a 62-30 win over the Knights Friday night in the first round of the PIAA Class A boys' basketball playoffs held at Clarion University.
Grumley swatted an opening layup from Aaron Lundmark off the opening tip.
"I don't know if that took them out of the game right there but it was a message, sure," Johnsonburg coach Bill Shuey said. "It was definitely a message."
[1] http://www.ridgwayrecord.com/sites/default/files/Mason Walters color.jpg
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|Monson, Mass.
|High School:
As A Freshman In 2011-12:
In eight tournaments and 17 rounds she averaged a 91.24, second on the Bobcats' roster...Followed a first-day 87 at the ECAC Division I Women's Golf Championship with a season-best 85 as she finished 43rd out of 69 golfers...At the Rutgers Women's Golf Invitational, she improved by five strokes (93-88) on the second day of the event...Shot a team-best 176 (+16, 89-87) at the 2012 Monmouth Hawk Invitational, finished at 29th out of 46 golfers...Shot a three-day 257 (+14, 85-85-87) at the 2012 NEC Women's Golf Championship, finishing 37th out of 53 golfers.
Before Quinnipiac:
Four-year golf letterwinner at Monson (Mass.) High School...Also lettered for six years in swimming...Captain for both golf and swimming...Honored with the Sportsmanship Award for golf swimming...Awarded the Coaches Award for golf.
Daughter of Tammy and William Russell...Has one brother, Ben, and one sister, Adriana...Majoring in nursing...Born in Pittsfield, Mass.


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Welcome back! This week is about saving with printable coupons.
"Printables" are coupons you can print from home via computer, and can be used the same as the ones you clip out of the newspaper. There are three main sources for printables:
Unfortunately, this month we will lose a fourth source, www.couponnetwork.com, as they are refocusing their activity to strictly Catalina coupons, which are coupons that are printed at check-out that may be used on a future purchase. So, what is a printable coupon and how do I use it?
It's really simple.
Go to one of the above sites, select the coupons you want, and click print. Initially, you will be required to install coupon printing software onto your computer. It is completely free and will usually allow you to print two copies of each coupon that you select per computer.
So, if you have two computers attached to one printer, then you can print four identical coupons. Each coupon will have a unique number assigned to it. Never photocopy printable coupons. Photocopying coupons is illegal and is classified as fraud.
The above sites reset/issue new coupons on the first day of each month. If there are any coupons that you want at the end of the month….Print! They may be gone the next day.
Coupons also have a print limit nationwide so it is possible that the overall limit can be met before you get it. If you see a high value coupon, print it or it may be gone. If you register with www.couponingtodisney.com and/or www.thekrazycouponlady.com, both post "hot" coupons on their blogs and Facebook pages.
Another great source for printables is company web sites. Betty Crocker, Bisquick, Pillsbury, and Kellogg's are just a few of the companies that have printables on their homepages and/or mail printables to your E-mail.
These require you to register with an E-mail account. Set up a free E-mail account to use for couponing and store clubs. This will keep your regular inbox from getting bombarded.
One last source for printables is store sites. Many stores and restaurants have printables on their homepages. Some require registration and some do not. Again, set up a separate E-mail account to use for money saving tactics. It will save your sanity.
Two of my favorite stores, Target and Wal-Greens, have printable coupons on their sites that you can stack with manufacturer coupons to score some great deals!
This was one of the biggest shocks I had when I started couponing.
Stacking is when you combine a coupon and other money saving strategies (cash back, store coupons) to maximize savings.
Now, the big question…What's the investment in ink and paper? It depends on how much you print and what you print. Here are a few tips to make sure printables are making money and not costing money:
• Only print what you will use
• Print in black
• Register for a rewards program at an office supply chain and roll the rewards! Rolling rewards means that you use reward money on offers that give you rewards back, thus eliminating the out-of-pocket expense. This is more "money recycling" than couponing, but the savings add up!
• Use a coupon swap group to attain extra coupons
So, now you may be asking yourself, "Can I still save if I don't have a computer or if I don't want to use printables?" Yes! In fact, I have a very dear friend who is an amazing couponer and she never uses printables.
Remember, it's not all or nothing. Find what works for you and your schedule. Any savings are better than none.
Next week we will explore digital savings. Please send all questions, comments, and/or tips to email@example.com. I look forward to hearing from you!comments powered by Disqus


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'Madame Mirage' #3 Preview
Our friends at top Cow have provided Comics Alliance with a 5 page preview of the new Madame Mirage #3.
Madame Mirage #3
(W) Paul Dini
(A) Kenneth Rocafort
(Cov) Kenneth Rocafort
Award-winning writer Paul Dini (DC's Countdown, Lost) and hot new talent Kenneth Rocafort (Hunter-Killer) answer the question - "Who is Madame Mirage?"
A locked room, two deadly adversaries, and one loaded gun between them. Madame Mirage finds herself in a life or death duel with ASI's most skilled assassin, and the


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If you can dream it, you can do it. -Walt Disney
Quality is a great business plan. -John Lasseter
Let's make some funny pictures. -Tex Avery
I never considered a difference of opinion in politics, in religion, in philosophy, as cause for withdrawing from a friend. -Howard Zinn
When critics sit in judgment it is hard to tell where justice leaves off and vengeance begins. -Chuck Jones
And what do you benefit if you gain the whole world but lose your own soul? -Jesus
A man should never neglect his family for business. -Walt Disney
What's most important in animation is the emotions and the ideas being portrayed. -Ralph Bakshi
Once you have heard a strange audience burst into laughter at a film you directed, you realize what the word joy is all about. -Chuck Jones
Before enlightenment: chop wood, carry water. After enlightenment: chop wood, carry water. -Buddhist Proverb
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Summer Thread
Showcase your drawings, illustrations, video and media.
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Please help! I need advice on drawing mickey!
http://i1261.photobucket.com/albums/ii5 ... f69eec.jpg
Re: Summer Thread
This is the best advice I or anyone else can give you at this point.
Take you time and focus on the quality of the drawing. Not how fast you can do it.
This is the next best advice...
Daily Z
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Heres another mickey!
http://s1261.photobucket.com/albums/ii5 ... ickey1.jpg
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Mind the messed up front legs of Tiger Tom
Re: Summer Thread
Tried gesture drawing, any advice?


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Edwyn Stephen Collins (born 23 August 1959) is an Ivor Novello Award winning Scottish musician, playing mostly electric guitar-driven pop. Collins formed the musical group Nu-Sonics in 1976, which later became Orange Juice. He has since pursued a solo career as a musician, in addition to work as an illustrator, television actor and producer, and as a record producer. He is most notable for his 1994 song "A Girl Like You".
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Designer Appliance Demo Days: May 22 in Southampton and Hanover!
Posted by Allison L. on May 20, 2010 at 14:47 pm
Have you been curious about designer appliances from brands like Sub-Zero, Wolf, or Viking and are interested in getting a closer look? Stop by our Hanover or Southampton Superstores this Saturday, May 22 from noon to 4pm to enjoy live demos, meet factory representatives, and have all your questions answered.
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Tags: Barbeques, Demonstrations, GE Monogram, Hanover, Jenn-Air, Miele, Southampton, Sub-Zero, Viking, Wolf
Filed Under: Appliances & A/Cs


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Submission + - Research: Tobacco Co.s Add Weight Loss Drugs->
smitty777 writes: According to this article from the European Journal of Public Health, the tobacco companies have been implicated in adding a number of drugs to tobacco products to enhance their weigh reducing properties. Discovery News did a good job of explaining the neurological process for appetite suppression, which involves activating pro-opiomelanocortin cells in the hypothalamus.
Link to Original Source
Link to Original Source


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U.S. Data
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Between 1990 and 2012, the U.S. population of immigrants born in Haiti tripled in size, from 200,000 to 606,000. This article provides the most up-to-date demographic information available for Haitian immigrants in the United States, including statistics on distribution by state and metro area, educational and professional attainment, income levels, health care coverage, and more.
The U.S. immigrant population—estimated at 40.8 million in 2012 — is the nation's historical numerical high, and it is also the largest foreign-born population in the world. About 20 percent of all international migrants reside in the United States, even as the country accounts for less than 5 percent of global population. This article presents the latest, most sought-after data on immigrants in the United States—by origin, residence, legal status, deportations, languages spoken, and more—in one easy-to-use resource.
In 2012, the United States granted humanitarian protection to more than 87,000 people, with grants of asylum up 19 percent and refugee admissions up 3 percent from a year earlier. This article provides a detailed look at the most recent refugee and asylum data in the United States, including country of origin, top states of settlement, and more.
More than 1 million people became lawful permanent residents (LPRs) of the United States in 2012, with family-sponsored immigrants accounting for two-thirds of those gaining a green card. This Spotlight examines federal statistics on foreign nationals who gained LPR status during 2012.
About 757,000 immigrants took U.S. citizenship in 2012, a 9 percent increase from the year before. As of 2012, 46 percent of the nation's 40.8 million immigrants were naturalized Americans. This article examines the latest naturalization data available for the United States, including historical trends, data by country of origin and state of residence, as well as socioeconomic characteristics of the 18.7 million naturalized U.S. citizens residing in the United States in 2012.
Recent Activity
A panel discussion on the release of the Regional Migration Study Group's final report, Thinking Regionally to Compete Globally: Leveraging Migration & Human Capital in the U.S., Mexico, and Central America, outlining its findings and offering recommendations to policymakers in the region.
This final report from the Regional Migration Study Group outlines the powerful demographic, economic, and social forces reshaping Mexico and Central America and changing longstanding migration dynamics with the United States. It offers a forward-looking, pragmatic agenda for the region, focusing on new collaborative approaches on migration and human-capital development to strengthen regional competitiveness.
This issue brief provides updated data, based on the Census Bureau's 2011 American Community Survey, on unauthorized immigrants in the United States, their demographic and socioeconomic characteristics, and their health care coverage. The analysis marks the first time that self-reported data on LPR status have been used to generate a national profile of unauthorized immigrants.
This report, Volume 1 of a three-volume set commissioned by the Winthrop Rockefeller Foundation that examines the immigrant population in Arkansas, provides a demographic and socioeconomic profile of Arkansas immigrants and their children, including a description of immigrant workers in the Arkansas economy. The three volumes build upon a previous study of the Arkansas immigrant population that was published in 2007.
Join MPI as U.S. Citizenship and Immigration Services Director Alejandro Mayorkas discusses the application process and policies that the agency has announced to implement the administration's deferred action program.
This fact sheet provides an estimate of the number of DREAMers—unauthorized immigrants potentially eligible for a two-year reprieve from deportation under the Deferred Action for Childhood Arrivals initiative—based on eligibility criteria outlined by the Department of Homeland Security. It also offers a detailed analysis of the demographic characteristics of prospective beneficiaries.
This report traces how the American public and the U.S. government have responded to the diversification of migrant streams and the increasing proportion of illegal immigration in recent decades. It outlines the anxieties triggered by this immigration, the policy response at the national and state levels, and the implications of the second generation over the long run.
This report provides a comprehensive profile of the immigrant population in Napa County, CA. It examines the effect of immigration on the county's demographic trends and calculates the economic contributions of immigrants as well as their impact on government revenues and expenditures.


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Choking Man
At the Olympic Diner, Jorge, the dishwasher, is choking on the American dream. Jorge is caught between his Ecuadorian roots, his new American life working at the diner for Rick and his own crippling shyness. When the diner adds a bright new member to the staff, Jorge's world gets a little more complicated as his affections grow for the new waitress, a beautiful Chinese girl named Amy.
Cast: Mandy Patinkin, Kate Buddeke, Eugenia Yuan, Aaron Paul, Octavio Gomez
Produced by: Steve Barron, Joshua Zeman, Zachary Mortensen
Directed by: Steve Barron
Written by: Steve Barron
Running time: 1hr 28min
Opens: Nov 09, 2007 NY
|Theaters for Today
Pick a day


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Internet Doomsday, July 9
Tweet Internet Doomsday! DCWG! What's all the fuss? This week's question comes from a retired high school principal who wants to know if DCWG is legit. Yes it is and a very helpful site to help you determine if you are part of the DNS Changer Trojan infection that is in the news and [...]Read More →


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Goergl takes second in Aspen
Lizzi Goergl dominated the first run of the World Cup Giant Slalom and held a lead of almost six tenths over the rest of the field. Yet the HEAD Rebel admitted after the second run that had seen her drop to second that she was not relaxed enough for the second run and was not able to better the formidable run that Viktoria Rebensburg put down to grab the win by a third of a second.
Goergl was at first annoyed that she had not won her third Giant Slalom yet after analysing her run and the performances of others was happy with second behind Rebensburg and ahead of Julia Mancuso.
While Goergl felt that she had not caught the line to the best of her ability on some of the gates, the disappointment of not winning soon faded and the infectious smile was soon there again. She admitted before leaving for Lake Louise that her imperious lead from the first run had not affected her mentally.
It was a solid day for the HEAD Rebels in Aspen as 22 year old Anna Fenninger also produced another fantastic performance. When she came down after her second run, Fenninger posted what was the fastest combined time to that point. Having safely negotiated the shadows that were appearing down the course, Fenninger had to wait and see how good her time would be. The fact that she was even at the start was remarkable as she had been suffering from stomach pains after the first run. Fenninger held on to take fourth overall. After having placed sixth in Soelden, Fenninger bettered this and was quick to point out that her "skis were working really well and this enabled me to attack," she explained.
For the winner in Soelden, Lindsey Vonn there was to be no double victory. Her first run was not up to her high standards and her second "felt better, I was definitely more aggressive, felt more like myself but it was just tough with the terrain and flat light, I just kept making mistakes but I did what I could and tried to make up some time. I think I did better but just not quite enough; first run was too far off."
With two races in the Giant Slalom season now completed, Goergl, Vonn and Fenninger sit in places two, three and four in the Overall and Giant Slalom classification. Next up is the Slalom in Aspen.
Results from Aspen
1. Viktoria Rebensburg (GER)
2. Lizzi Goergl (AUT) HEAD
3. Julia Mancuso (USA)
4. Anna Fenninger (AUT) HEAD
12. Lindsey Vonn (USA) HEAD
22. Anne-Sophie Barthet (FRA) HEAD
23. Giulia Gianesini (ITA) HEAD


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Now we're email buddies!
You have chosen to receive our #NAMED_LISTS# newsletters at #EMAIL_ADDRESS#. You will receive an e-mail shortly confirming your subscription. So excited to have you!
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Charli XCX keeps it real. She's stylish, funny, smart, makes no apologies, and her music is as deep as it is catchy. At only 19-years-old, this British singer-composer isn't exactly a newcomer to the music scene (her first single was released way back in 2008), but excitement about her debut record has grown pretty much continuously with releases like last year's "Nuclear Seasons" and this year's "Valentine."
While other artists worry about what people will think of them, Charli XCX speaks off the cuff, with the laidback confidence of a young woman who is equal parts songwriter, party girl, and performer. When she talks about how she used to want to be a rapper, it's with the same sincerity and humor as when she mentions embarrassing herself in front of Baby Spice. There isn't a different person waiting to be unearthed behind her trademark black eyeliner (although we do learn the secret behind it); With Charli XCX what you see is what you get, and that might make her the perfect pop star.
Makeup by Sonia Bhogal using MAC Pro
Photographed by Kate Cox
Photographed by Kate Cox


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Goals: Asiasat 3 was an accidental Moon mission. A communications satellite launched for China, a booster rocket malfunction failed to put Asiasat 3 in a stable Earth orbit.
Accomplishments: Using techniques pioneered by space science missions, engineers sent the spacecraft on two flights around the Moon to put it into a stable orbit at Earth. It was the first time such a maneuver was performed by a commercial satellite. The spacecraft has since been bought and sold and renamed.


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Introducing ContourSM from Cox
Contour is TV Just for Me
Imagine TV as you've never seen it before. Contour is reshaping TV to perfectly match you. Everything you love, here or there, in any order, any time you want. It's new, it's revolutionary and every part of it is totally personalized just for you. Welcome to Contour.
A TV Experience that Perfectly Fits You
Contour is a complete TV-viewing experience. Innovative features make watching at home, online and on the go more about you than ever before.
- The app's intuitive interface serves up the shows most relevant to you with a simple swipe or tap of your finger.
- Contour on your TV learns what you like and brings you new things you will love.
- The Record 6 DVR lets you record six shows at once and store 1,000… with one DVR.
Personalization Like You've Never Seen
TV has always been about entertainment. With Contour, it's about your entertainment. Take control and discover a whole new way to watch TV as Contour adapts to you.
Search less. Discover more.
With personalized recommendations, Contour brings you shows you like and recommends new shows you may love. Follow the green diamonds to a TV experience shaped perfectly to you.
Record six shows at once and store 1,000.
Add the Record 6 DVR to record up to 6 shows at once and store up to 1,000. Plus, you can watch recorded shows on any TV in the house. Start watching a recorded show in one room and resume watching it in another. All with one DVR.
A TV Experience that Follows You
Your favorite shows, anytime you want them. You can watch live shows, choose personal favorites and access thousands of On DEMAND titles anywhere in your house, right on your iPad. You can also access TV network mobile apps and watch anywhere. It's all at your fingertips on the Contour app.
An App Like No Other
Intuitive. Adaptive. All for you.
Tap, swipe and slide your way to personalized TV experiences. The Contour app serves up the content most relevant to you.
Get the big picture.
With Contour, your iPad® and your TV work together. Find a show on your iPad and send it to the big screen with just one tap.
Get more control.
Contour lets you create up to eight individual profiles, so each member of the family can have their own personalized TV experience.
Maximize your at-home viewing experience with a home theater.
Get ready for a lesson in what's so hot about sports with Cox... and a healthy dose of smack talk.
Forget the movie theater! Experience 3D at home with Cox Advanced TV.
People love Facebook or they hate Facebook. Whatevs, as the kids say.
Get pointers on how to best use your clicker.
Learn how to get the most from your TV service with the pros at Cox.
The pros at Cox give their tips on setting up your home network.
Maximize your at-home viewing experience with a home theater.
Learn how to get the most from the Web with the pros at Cox Communications.
Setting up parental controls is easy with Cox.
Worried about online safety? Learn about security from the Cox pros.
Let your Cox services help keep your family bond strong, even if you're not all under the same roof.
Find out how this family stays connected across the country.
Find out how this family uses Cox services to stay ahead of the curve.
Bring it all together to stay in touch with the world.
If music is your life, Cox services can help you live it.
How can you keep your kids safe with so much out there?
Get ready for a lesson in what's so hot about sports with Cox... and a healthy dose of smack talk.


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Maps and Directions
Research Study Will Compare Two Treatments for Lung Cancer
The STARS (Stereotactic Radiosurgery vs. Surgery) trial will compare the three-year survival rate of patients who undergo a lobectomy, a surgical procedure to remove the tumor, versus CyberKnife treatment, a unique stereotactic body radiotherapy technique that precisely tracks and focuses the radiation on the tumor.
"Surgery is the current gold standard treatment for Stage I NSCLC. However, there are research reports that describe similar survival rates of operable patients treated with the CyberKnife," said John Dombrowski, M.D., Ph.D., assistant professor of radiation oncology at Saint Louis University School of Medicine.
Previous page: CyberKnife Tour SLU Hospital
Next page: Walking Map


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Fulfill every runner's fantasy of running through an African game park amidst wild animals on the open plain. We are pleased to offer our 14th trip to Kenya for the Safaricom Marathon and Half-Marathon on June 27, 2015. Our trip in 2014 was another huge success. We are limited to 40 travelers on the basic itinerary and 20 guests at Governor's Camp since our chartered planes have seat limitations. The event was conceived in 1999 by Tusk Trust, a London-based charitable organization dedicated toward preserving wildlife in its natural habitat. The event, the only marathon run in a game park, is run on dirt roads within the 62,000 acre Lewa Wildlife Conservancy. The two-looped course offers some of Kenya's most beautiful scenery with Mount Kenya lying 20 miles to the south and Samburu National Park and Mt. Lololokwe to the north. Click here for a video on YouTube. Lewa is home to over 125 black and white rhino, herds of elephant and a vast assortment of plains game including oryx, eland, zebra, giraffe, buffalo and more. Lion, leopard and cheetah also can be seen. There were 8 rhino born in Lewa in 2011 which you will enjoy viewing. The course is patrolled by a spotter plane and helicopter as well as a large and experienced team of armed rangers to avoid any confrontations with the four-legged residents. There are manned aid stations every 5K and water stations every 2-1/2K. The local Masai and Samburu communities will not only run alongside visitors but will also entertain participants during race day.
We are pleased to announce that we will spend two nights at the newly-renovated Aberdare Country Club. Extended stays are available for further excursions to Masai Mara, or perhaps, a Kilimanjaro climb in Tanzania. How about gorilla trekking in Rwanda or Uganda? We had nine guests experience this unique adventure in 2012. They were astounded!
This trip is very limited due to the accessibility of the area and the limited accommodations. A minimum fund raising amount of $1,500.00 per person is required per entry for TUSK, a long-term conservation partner to the Lewa Wildlife Conservancy.


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In this Instructable I will teach you everything you need to know to put together a portable. There will be several different sections:
- What batteries to choose
- How to make a case
- How to wire everything
- What screen to get
These are just the basics. All these and much more will be discussed in this Instructable.
The console highlighted in this tutorial is the Nintendo Entertainment System. I chose it because it has tons of awesome games and it is easy to make handheld. The total cost of this project - for me at least - was $200. Now don't freak out - your price will be much lower. It was so high for me because I broke the first screen and NES. That set me back about 70$. :P You won't make the same mistakes, because you are going to learn from mine.
Now, this project is not for the lazy. It is not easy. It is not cheap. But the reward is worth so much more than what you spent, that you won't even care. :) My portable NES took me about 4 months to build, so lots of dedication is needed.
This Instructable will play with a new format; because the parts list is so huge, it would be ridiculous to post it all in one step. So, for each individual step, I will write the parts and tools needed for that one step. I will tell you one thing: get some IDE cable. That's the stuff that is used to connect hard drives inside of computers. IDE cable is invaluable to any portable. It's amazing stuff, and you will use it many times in this project. Get some in advance.
This project requires that you already have the basic knowledge to solder, use tools, simple stuff like that. This guide assumes, however, that you would have no clue where to start when building a portable. This tutorial is for you.
I urge you to read the following steps on picking and ordering a console, screen, and batteries. They contain very useful information and will help you greatly. It may seem like a lot to read, but please look at it all.
Now, let's finally get to the Instructable. :)
I'm sorry if you have already mentioned this but can you tell me what device your battery is from?
If you're looking for a slightly cheaper screen, I'd recommend this:
It was meant for the Raspberry Pi, but it would work great for this project!
If you're looking for a slightly cheaper screen, I'd recommend this:
It was meant for the Raspberry Pi, but it would work great for this project!
Do you have any idea if this would work with a gamecube controller?
I have a question...
Online I've seen many portables (mainly from GManModz on YouTube) and I've been wondering. This instructable covers making the circuit board flatter okay however what if you want to make it smaller? Because that portable is Huge! and I've seen other portable that are way smallers. (Darth64, GC-Lynx to name a few) so could you please help me?
you wont sell it??
You mean NES, right?
Yes it would. I used a very similar screen on mine and it worked just fine.
Hey, i am trying to make one, but i can't figure out how i would connect an AA bettery pack up to the n64, could i solder it to the AC wire???, do i take out the AC connecter and just solder the leads to the AA straight to where the AC connecter?
If I just wanted to attach the PSOne screen to my NES, is the wiring pretty much the same? To use the PSOne screen's audio and video I mean? And would the NES's original RCA jacks still be usable? Thanks in advance!
it worked for me
Thanks for the great instructable! I'm not planning on making a portable soon, but I just happened upon a couple of NES boards without cases, and was thinking about making cases for them, and putting a standard cartridge port out the back instead of messing with the finicky ZIF socket.. Your information on where to get the ports is very helpful.
Also, could you let me know where the wires from the power and reset buttons on the case go to the blue connector on the NES motherboard? I don't have a case to check, and I'll be needing to add my own power and reset buttons.
also i reverse engineered a portable dvd player lcd to work without the dvd player control circutry and it now takes composite. 7 inch diagonal screen
The detailed explanation is a great bonus.
Excelent work!
It also has some garbage showing up in bottom border area but that is from a factory glitch in the Genesis hardware and thus unsolvable unless I start replacing chips.


text: This striking halo engagement ring features 40 round pavé-set diamonds, at approximately .44 carat TW, and two baguette diamonds, at approximately .09 carat TW. The stones have been hand-selected and matched for exceptional sparkle having a combined total gem weight of approximately .53 carat. Set in superior quality 14 karat white gold, this split shank ring awaits the center diamond of your choice at approximately .75 carat.Item Number: 41045628


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Terms & Conditions.
Some product exclusions may apply. See product page details for more information. Clearance items are final sale. Price matching does not apply to this or any promotion. Offer is valid until September 13 at 11:59 PM PST. May not be combined with any other offer/discount (unless specified), including invitation credits, promotional gift cards and/or special discount links. Discount applied upon checkout. Log in to your account to see your savings.
What could be more fitting for your aspiring rock-god cousin than this Electric Picks Bracelet? It's eye-catching and conversation-starting, with brass chain links and a signature Electric Picks guitar pick, all hand-knotted on an elastic cord.
Authentic guitar pick bracelet. Handmade in New York City. 7" long, elastic.
Inspired by the timelessness of rock music, event planner-turned -designer MJ Barton created Electric Picks in 2011 with a very specific attitude in mind. Materials inspired by her world travels are mixed with gemstones and undeniably rock 'n' rol
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Nb: infants must be under the age of 2 and
children must be under the age of 12 at the time of
Beautiful small apartment house "Pfingsteggblick", built in 2008. In the district of im Graben, 200 m from the centre, south facing position. In the house: storage room for skis, central heating system, washing machine, tumble dryer (for shared use), drying room. Motor access to the house. In Winter: please take snow chains. Parking at the house, communal covered parking. Grocery 200 m, shopping centre 400 m, restaurant, bar 300 m, bakery 100 m, railway station "Grindelwald Dorf" 900 m, outdoor swimming pool 2 km, indoor swimming pool 800 m. Golf course (9 hole) 800 m, tennis 500 m, minigolf 700 m, sports centre 1 km, gondola lift, ski rental 400 m, ski bus stop 20 m, cross country ski track 500 m. Please note: no lift. Ski bus (free of charge). "Pfingsteggblick", 2-room apartment 39 m2, on the ground floor, south facing position, modern and cosy furnishings: living/dining room with cable TV and radio. Exit to the terrace. 1 room with 2 beds (90 cm, length 200 cm). Open kitchen (oven, dishwasher, 4 ceramic glass hob hotplates, microwave, electric coffee machine, raclette grill), fondue Set (cheese). Shower/WC. Floor heating. Patio, south facing position. Terrace furniture. Very beautiful view of the mountains. Facilities: Internet (WiFi, free). Reserved parking, garage space n 1 & 2 at the house. Please note: non-smokers only.
Iglu acts as a travel agent for disclosed principals/tour operators, further details
of which will be made available at the time of booking. Your holiday contract will
be bound by the tour operators terms and conditions.
Prices are shown for a basic room or unit, can be subject to change and are indicative
until time of booking. Please call on 020 8544 7114 for prices for other travel
and room options for this property.
The price shown includes 7 nights accommodation,
on an accommodation only basis.
Prices shown are per person per week based on maximum occupancy. Descriptions and
quality ratings are provided by the Tour Operator (or one of the Tour Operators
where similar holidays are offered by more than one supplier). We reserve the right
to make minor adjustments to the description or rating based on staff and customer
feedback. For payments by credit card, a 2.5% credit card charge (4% for AMEX) will
be added to your invoice. If you pay by a UK issued debit card, there is no charge.
All bookings are subject to a £5 per person Passenger Admin Fee charged by Iglu.com.


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Nike Youth Home Game Jersey Cincinnati Bengals Rey Maualuga #58
Product Information:
Chase your youngster around the fields of play this season with the Nike® Cincinnati Bengals Rey Maualuga #58 Home Game Jersey. The jersey's lightweight, athletic fit and strategically placed ventilation let even the most active child run around the yard in comfort all afternoon long. Dress your little Bengal just like his or her favorite linebacker with Maualuga's name screen-printed on the back and #58 on the front, back and shoulders.
Click here to explore different features of Nike NFL Jerseys.
- Youth Cincinnati Bengals Rey Maualuga #58 game jersey
- Lightweight, athletic fit supports even the most active child
- Maualuga's name screen-printed on back and #58 on front, back and shoulders
- Bengals logo screen-printed on the upper chest
- Swoosh™ design trademark screen-printed on the sleeves
- TPU NFL Shield on the V-neck collar
- Strategically placed ventilation panels for breathability
- Satin twill woven jock tag at lower left hem
- Tagless neck label for comfort
- Conveniently tailored to fit both boys and girls
- Machine washable, cold
- Fits youth sizes: 8-20
- Officially licensed by the NFL
- Fabric: 100% polyester
- Nike
Country of Origin: ImportedWeb ID: 15556196


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Invitations & Stationery
Invitations are the perfect way to let your guests know the tone, mood and theme of your wedding and party in Andersonville, Georgia. You will be able to wow your guests from the very beginning with elegant wedding invitations or themed invitations. Party invitations can represent exactly who you are and how your wedding will be, whether it is more formal, casual or a vintage 20's theme. Themed invitations are great ways for getting your guest details and the theme of the party. Invitations are for all occasions including, bridal shower, graduation and birthday party invitations. You will be able to find all sorts of wedding invitations to fit your style at your closest invitation store.
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A day of golf, camaraderie, competition and community support for Orlando Science Center.
Hosted by Bright House Networks
Thursday, March 26, 2015
We welcome back our loyal supporters and invite new sponsors and players to join us for the 31st Annual Cosmic Golf Challenge. Not only will your donation have a direct impact on the children of Central Florida but it is also an opportunity to showcase your company to Orlando's business leaders and professionals.
Heartfelt thanks to Bright House Networks and all the companies and golfers providing support for the Orlando Science Center today. The Science Center served over 413,000 learners last year, including 90,000 youth. Cosmic Golf Challenge provides funds to further our mission to inspire science learning for life.
When: March 26, 2015
Where: Walt Disney World Golf Magnolia and Palm Courses
Format: Four Person Scramble
Entry Fee: $550 per player or $2,000 per foursome
Tournament Schedule
Registration: 11:30 a.m.
Shotgun Start: 1:00 p.m. Sharp
Dinner in Club House: 6:00 p.m.
Raffle Prizes Distributed: 6:00 p.m.
Awards Presentation: 6:45 p.m.
To register to sponsor or play in the Cosmic Golf Challenge
Download our Sponsors Form.
27 March 2015
Posted in Cosmic Golf Challenge
More than 200 golfers took a swing for science on March 26, 2015 during the 32nd Annual Cosmic Golf Challenge at the Walt Disney World Golf Magnolia and Palm Courses. Sponsored by Bright House Networks, the event raised funds for youth education programs at the Science Center. Various corporate teams and individual players came together to putt for progress over 18 holes of best ball tournament play on two separate courses.
Hole sponsors, "mulligans" available for purchase and raffle prizes generated revenue, and professional golfers could step in to swing for a donation. Players enjoyed lunch, dinner, an awards ceremony, drawings, contests and prizes. A special thank you to Bright House Networks, Jaguar Technologies, KableLink Communication, Knight Enterprises, Noble Resources, Oakstone Capital Partners and Structured Broadband Services for their generous and ongoing support of the Orlando Science Center.


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Electronics Buyer's Guides
The enhanced viewing experience of a 4K HDTV can bring true cinematic experience into your home theater, but before you make that investment in a new TV it's important to understand what exactly 4K can do for you.
Smart Televisions connect to the internet and allow you to access online media, such as movies, music and social media.
LED vs LCD HDTV: What's the Difference?
The first thing you should realize is that they both have the same type of screen. The difference is actually behind the screen, in the technology used to light up the image.
These devices are split up into four main categories: midsize laptops, desktop replacements, portable laptops and tablets. These common categories also correspond to different price ranges.
What should you look for when buying digital camera? The first decision to make is whether to buy a point-and-shoot camera or a digital SLR camera.
Shop by Brand
Whether you're buying a laptop for work or putting together the ultimate home theater, Kmart's wide selection of consumer electronics has what you're looking for. Here in our online electronics store, you'll find the latest computers, TVs, digital cameras and much more.
Browse our personal computers and take your pick from the best names in the business. If you're looking for something lightweight to carry on your daily commute, pick up a sleek HP laptop. If you're putting together a dedicated gaming rig, pair a powerful Lenovo desktop with premium Logitech computer speakers for a completely immersive experience. No matter which option you choose, it's easy to stay connected at great prices with a new computer from the Kmart electronics store.
When your friends are coming over to watch the championship game or have a back-to-back movie marathon, you want a home theater system that's up to the task. Kmart offers everything you need to wow your guests with the ultimate viewing experience. Make some popcorn, relax with your company and enjoy the crystal clear picture of your new Samsung HDTV, combined with the impressive acoustics of your LG TV speakers.
Kmart gives you an affordable way to equip every room in your home with the most advanced electronic gear. You'll get cutting-edge tech at the best prices when you shop for electronics online at Kmart.


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User Tip: If you're unsure of your vehicle type, you may skip the machine type selection box.
Select type, make, model and year above
"Customer service is priority #1 at Rocky Mountain ATV/MC. We value our customers and strive to be the best shopping experience- when you buy from us, you are buying lifetime service."
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We take pride in offering no hassle returns. In the event of a return, please call, email or chat with us to receive a solution to the problem. For apparel that doesn't fit quite right, Click here to see our Fit Guaranteed Program.
We will stand behind all products we sell with regards to manufacturing defects in workmanship and material. Most products are limited to the manufacturer's warranty. However, we will do everything in our power to take care of the problem.
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Comments On Dec 11, 2013:This wind shield is awesome, what a difference it made from the stock one. It eliminates the wind from swirling around your helmet causing excessive wind noise. A must if you want less wind around you when it is in full extended position.
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Comments On Nov 13, 2010:I bought this for the Concours 14 and I am very happy with how it performs. Definently worth the money, it keeps all the air off my torso and I get a little bit of buffeting on the top of my helmet. I am 6'2'' 250 so there is quite a bit of me to keep the wind off of.
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12 ChairsFebruary 15th, 2010 4:00 am - 10:00 am
Tickets: $ 35
21 and Over
56 MacDougal Street
New York, New York
Phone: 212-211-2323
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Trey Songz Falls Into Audience of Adoring Female Fans in Detroit
Whether we're talking boyfriends and girlfriends, husbands and wives or artists and their fans, trust is the cornerstone of any solid relationship. In that sense, Trey Songz has nothing to worry about.
On a recent visit to Detroit, where he promoted hi


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In a few areas on my car I have used my MM210 Mig to weld in patch panels on the car, tacking every inch or so, jumping around and letting it cool. This has worked, but I was not crazy about all the grinding that needed to be done afterward (maybe caused by my lack of experience).
So I was tacking a patch panel in on Saturday on my rusty quarter panel with the Mig and thought "I wonder if the Tig machine would do this a little cleaner"
So before I move forward to use my new Tig machine on the panel, I plan on practicing a good amount on some scrap sheetmetal of similar thickness. My question: is there anything I should do specifically in Tigging thin body panels? For example, is it best to tack similar to Mig, or run a continuous bead with the Tig? I realize heat is the enemy here and do not want to warp the panel, but was concerned that even a real low amperage setting may warp the panel with a continous bead.
Results 1 to 10 of 24
08-03-2009, 06:03 AM #1Junior Member
- Join Date
- Jun 2008
- Posts
- 17
Need some advice TIG welding patch panels
TIG will have less to grind down, but you need the panels to fit TIGHT to do this. (like 0 gap) Practicing on scrap is always the best idea. This way you can get a good setting before you tackle the real thing. But, even with less amps you can warp the panel using TIG compared to MIG. The main reason is speed. The MIG is done as soon as you pull the trigger for tack welds. The TIG can take twice as long to tack for the novice. Even with the TIG you can't run long beads on thin stuff. Having a wet rag next to you to dab the weld right afterwards helps some, but can make the metal brittle if too much heat was applied.
Do you have .023 wire in your MIG? It works the best for body panels. For grinding down the weld I use a thin (3/32nd thick) cut off wheel in a die grinder, but a 4 1/2" angle grinder with a cut off works also. This way you only remove the filler not the metal in the panel, and it keeps the heat down.Dynasty 200 DX runner
Sync 350 LX
XMT 300 w/D74 and roughnecks
Hobart 135
ESAB PowerCut 875 plasma
08-03-2009, 12:07 PM #3Junior Member
- Join Date
- Jun 2008
- Posts
- 17
Thanks. Yeah, I have been using .023 wire in the Mig. Good tip on grinding, will have to try that. I just figured this was a perfect application for me to put the new machine to use on. I realize it will be slower, but if the end product is better or at least less painful to get to (ex:less grinding), I thought it would be worth it.
08-03-2009, 04:45 PM #4Senior Member
- Join Date
- Jul 2007
- Posts
- 241
If you are tig welding in patches On your ''rusty quartepanel'' you better cut your panel back to clean steel if you plan on making good welds with tig. The areas where you plan to attach donor panels to will probably have some porosity and contamination which will defeat the purpose of tigging them.You are going to fiind out first hand why body shops use mig instead of tig.if tiigging was the way to go more shops would do it.Also butt welded panels have to be fit up perfect or you will burn lots of holes trying to get a puddle started,If the fit up is perfect you can make some very nice precision welds on 18 guage.Just think that if the grinding is getting to you,how about the Body filler and 2K block sanding you will have to do to get the panel smooth? I dont think the tigging is the answer that you may think it is for auto sheetmetal.Mike
The blocking and priming isn't an issue. Not that I dislike the grinding, just makes me nervous that I spent a lot of careful time trying not to distort the area during the welding process that I am afraid I will create too much heat during grinding. I have seen it happen on a friend's car. For some reason he did not realize the amount of heat that builds up, had to do a lot of hammer/dolly work to get it back. I figured the Tig may help reduce that chance, but you do bring up some really good points.
I have the Tig machine right next to the Mig, so I will practice on a couple of scraps at first and see how I feel. May not even mess with it on the car if I do not feel comfortable.________________
Miller 210
Diversion 165
You will have to do some hammer and dolly work from the welding reguardless, but using cut-off wheels for the grinding eliminates heat during that part of the work.Dynasty 200 DX runner
Sync 350 LX
XMT 300 w/D74 and roughnecks
Hobart 135
ESAB PowerCut 875 plasma
08-03-2009, 08:19 PM #7Junior Member
- Join Date
- Aug 2008
- Location
- Macon, Ga
- Posts
- 13
I use both mig and tig for body panels. I prefer the tig for small patches and mig for larger work like quarter panels and floors.
I also like the 4 1/2 flapper disk over the grinding disk, it also helps to move around while grinding just like you do when welding.
Hope this helps DD
Depending on the panel size I use the "flapper", or 2" 50 grit disc on air grinder after getting the bead knocked down close to panel with the cut-off wheel.Dynasty 200 DX runner
Sync 350 LX
XMT 300 w/D74 and roughnecks
Hobart 135
ESAB PowerCut 875 plasma
I TIG welded this crotch rocket fuel filler cap into my Jeep CJ-5 body.
I used a butt weld and fit it up good, bet I didn't have to use a 1/2 teaspoon of body filler! Go slow, allowing your welds to cool, skip around, use .040 tungsten for the thin stuff.Miller Syncrowave 200
Homemade Water Cooler
Spectrum 375
60 year old Logan Lathe
Select Machine and Tool Mill
More stuff than I can keep track of..
All great advice, thanks guys. This coming weekend I will mess with both. Since I have one part of the quarter already patched using the Mig, I still have to grind down the welds and will using the advice you guys provided. I will also spend some time messing with some thin scraps with the Tig and see how I do. Who knows, maybe it will work out better for me, at least it will give me more of a reason to tell the wife I need to go out in the garage to practice more________________
Miller 210
Diversion 165


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Rise of the Guardians IMAX 3D
Action/Adventure, Animation
Rise of the Guardians tells the story of a group of heroes -- each with extraordinary abilities. When an evil spirit, known as Pitch, lays down the gauntlet to take over the world, the immortal Guardians must join forces for the first time to protect the hopes, beliefs and imagination of children all over the world. In 3D at select locations.
Cast: Jude Law, Hugh Jackman, Alec Baldwin, Chris Pine, Isla Fisher
Produced by: Christina Steinberg, Nancy Bernstein
Directed by: Peter Ramsey
Written by: David Lindsay-Abaire
Running time: 1hr 37min
Opens: Nov 21, 2012 Limited
|Theaters for Today
Pick a day
on this day.


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Nb: infants must be under the age of 2 and
children must be under the age of 12 at the time of
The Alpenhotel Tauernkönig is popular for its warm atmosphere, good service and rustic charm, it houses the lively Hasenstall après-ski bar and the Königsalm restaurant and pizzeria.
44 Bedrooms.
Bedroom facilities
Iglu acts as a travel agent for disclosed principals/tour operators, further details
of which will be made available at the time of booking. Your holiday contract will
be bound by the tour operators terms and conditions.
Prices are shown for a basic room or unit, can be subject to change and are indicative
until time of booking. Please call on 020 8544 7114 for prices for other travel
and room options for this property.
The price shown includes 7 nights accommodation,
with flights from Heathrow.
Prices shown are per person per week based on maximum occupancy. Descriptions and
quality ratings are provided by the Tour Operator (or one of the Tour Operators
where similar holidays are offered by more than one supplier). We reserve the right
to make minor adjustments to the description or rating based on staff and customer
feedback. For payments by credit card, a 2.5% credit card charge (4% for AMEX) will
be added to your invoice. If you pay by a UK issued debit card, there is no charge.
All bookings are subject to a £5 per person Passenger Admin Fee charged by Iglu.com.


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Virtua Fox Chase Cancer Program
Topics in This Section
Contact Fox Chase Cancer Center Partners
Contact the Partners
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Directions to Fox Chase Cancer Locations
Directions and Maps for Fox Chase locations
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Perhaps the most critical step a person with cancer must take is to find a cancer program that combines sophisticated treatment with personalized care. That's why the highly skilled team at the Virtua Fox Chase Cancer Program offers each patient an individualized care plan and a coordinated program that ranges from cancer prevention, education and screenings to leading-edge treatment, including cancer prevention and treatment trials.
Virtua Fox Chase Cancer Program features on-site surgical, chemotherapy and radiation oncology services, inpatient oncology units, a comprehensive rehabilitation program, nutritional counseling, pastoral care, and social work services including support groups for patients and families. What's more, patients have access to second opinions on site, in New Jersey, provided by Fox Chase Cancer Center in Philadelphia.


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Celebrate the beauty and athleticism of the marquee event of the Winter Olympics! Straight from Turin comes "Figure Skating", one of seventeen official posters of the Torino 2006 Olympic Winter Games. With artwork created by the world-famous Iconologic brand design firm, this entry in the collection captures the sport with a colorful modernist style that looks fantastic on the wall, evoking all the legendary evening programs that provide memories of a lifetime. With a crisp white border, the official Torino 2006 logo as created by the Italian design firm Agenzia Armando Testa, and trilingual titling (Italian, English, and French), this is a beautiful reminder of the greatness of the Olympic movement and the athletic achievements that thrill the world. Imported directly from Italy, this is a SportsPosterWarehouse.com North American exclusive, and a rare collector's item likely to rise in value as the years go by!
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Frank Ocean: Five Things To Know
December 30, 2013 via Pepsi
Frank Ocean: Five Things To Know
Frank Ocean emerged at a time in music where image seemed to trump content. Acts—whether they be solo or groups—engaged in a flawed game of show-and-tell in which they attempted to demonstrate mastery simply by explaining all of the "excess" that comes along with being in the business. Ocean's poignant and refreshing take was a breath of fresh air for music lovers who were growing tired of the status quo and longed for a reliable orator for more universal topics. Despite still being in the early stages of his career, Ocean compiled an impressive catalog of songs ranging from mixtape classics to more introspective numbers pulled from channel ORANGE. Despite living his life in the public sphere, Ocean managed to keep an air of mystery about him. Here are five things you may not know.
1. He politely turned down the opportunity to have Kanye West on channel ORANGE.
Despite lending his voice to "No Church in the Wild" and "Made in America" from Jay Z and Kanye West's collaborative project Watch the Throne, Ocean decided that he didn't need West's services on his debut album. In an interview with Zane Lowe on BBC Radio 1, he told the host that "I said, 'As much as I want to work with you…I kind of want to do this without you. I kind of want this to be done without you. I kind of want to do it on my own.'"
2. He got his start as a songwriter for other artists.
Before he struck out on his own, Frank Ocean honed his own chops penning songs for other artists like Justin Bieber, John Legend, Brandy, Jamie Foxx, Alicia Keys and Beyoncé.
3. James Ryan Ho, better known as Malay, had a major impact on his career.
Serving as the producer, writer and engineer on 13 songs from channel ORANGE, Malay and Ocean were first introduced through their publisher. In speaking about their work relationship with Complex he commented, "I met him before the whole Frank Ocean thing years back. At the time both of us were kind of writing little one-offs here and there. We did some Mario and John Legend records—just little placements here and there. I met him when I was in Atlanta."
4. His cover of Coldplay's "Strawberry Swing" and The Eagles' "Hotel California" had drastically different feelings from the original artists.
Chris Martin was so impressed with Ocean's rendition of "Strawberry Swing" that he invited him on tour to be a part of their European leg of shows. Conversely, Ocean's "American Wedding"—a melodic twin to "Hotel California"—was met with utter disdain. An Eagles legal representative released a statement saying, "Frank Ocean did not merely 'sample' a portion of the Eagles' 'Hotel California,' he took the whole master track, plus the song's existing melody, and replaced the lyrics with his own. This is not creative, let alone 'intimidating.' It's illegal."
5. He goes by "Lonny" to friends.
Despite the name change that took him from Christopher Breaux to Frank Ocean, The Fader reports that he is merely known as Lonny to close friends.


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SAR analysis of Antagonists of the GPR35 Receptor using an Image-Based Assay - Set 5
Assay Provider: Dr. Mary Abood, (Temple University, formerly at California Pacific Medical Center Research Institute) ..more
BioActive Compounds: 10
Related Experiments
Data Source: Sanford-Burnham Center for Chemical Genomics
Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA)
Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN)
Grant Number: 1X01 DA026205-01
Assay Provider: Dr. Mary Abood, (Temple University, formerly at California Pacific Medical Center Research Institute)
Addictive behavior stems from abnormal signaling activities in the brain. Thus identification of compounds blocking this modified signaling activity may lead to treatments for addictive behavior. GPR35, a to-date uncharacterized orphan G-Protein Coupled Receptor, is thought to play a role in addiction and has homology to other known receptors of abuse.
This high-content imaging assay is developed and performed to confirm activity of hits originally identified in a high-content screen for antagonists of the GPR35 receptor, "Image-Based HTS for Selective Antagonists of GPR35" (AID 2058) and to study the structure-activity relationship on analogs of the confirmed hits. Compounds are either acquired from commercial sources or synthesized internally.
The assay will identify small molecule antagonists of the GPR35 receptor. This imaging assay utilizes a cell line permanently expressing a beta-arrestin GFP biosensor and the GPR35 receptor. Upon agonist-mediated GPCR activation, the arrestin-GFP redistributes from the cytosolic compartment to the plasma membrane and further into coated pits, which can be quantified as increased local aggregation of the GFP-arrestins.
Assay Materials:
1) 384-well plates, black with clear bottom (Greiner# 781091)
2) U2OS (Human Osteosarcoma) cell line stably expressing the Beta-arrestin GFP and GPR35 receptor
3) Culture Media: MEM with L-glutamine, Pen-Strep, 10% Fetal Bovine Serum, 10mM Hepes, and selection antibiotics - 200ug/ml G418 and 50ug/ml Zeocin
4) DMSO Working Solution: 100% DMSO
5) Agonist Working Solution: Zaprinast (Alexis #ALX-430-020-M010,10mg) 5mM stock in DMSO diluted to 100uM in PBS.
6) Test compounds from dry powder working solution: 10mM in 100% DMSO
7) Fixative Working Solution: 6% Paraformaldehyde (PFA) diluted in PBS.
8) Nuclear Stain Working Solution: DAPI (Invitrogen, D1306) diluted to 150ng/ml in DAPI buffer (10mM TRIS, 10mM EDTA, 100mM NaCl, pH 7.4).
Assay Procedure:
1) 45ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser.
2) Plates are incubated overnight or approximately 20 hours at 37 degree C and 5% CO2.
3) Serum is removed by media aspiration and replacing with 45ul serum-free MEM prior to addition of compounds.
4) Compound addition was done on the ECHO550 Liquid Handler. The "dose response protocol" was used to dispense corresponding volumes of each 10mM compound on the assay plate.
a. Compounds were added to columns 3 to 22. Final concentration ranged from 62nM to 32uM (ten doses), in duplicate.
b. Agonist working solution (Zaprinast) was added to the entire plate except columns 1 and 24 (DMSO only as positive non-inhibition control). Zaprinast final concentration was 10uM.
c. DMSO was added to column 1, 2 and 23, 24. Final DMSO concentration was 0.31%
d. DMSO was back-filled to each well to achieve a 0.31% final concentration.
5) Plates were incubated for 45 minutes at 37 degrees C and 5% CO2.
6) Media was aspirated leaving 20ul liquid in each well using a Titertek plate washer.
7) 40ul of fixative working solution was added to each well using a Wellmate bulk dispenser (Matrix) for a final concentration of 4% PFA.
8) Plates were incubated for 40 minutes at room temperature.
9) Fixative was aspirated and plates were washed twice with 50ul PBS leaving 20ul liquid in each well using a Titertek plate washer.
10) 40ul of DAPI working solution was added using a Wellmate bulk dispenser for a final DAPI concentration of 100ng/ml. Aluminum plate sealers were applied to each plate.
Image Acquisition and Analysis:
1) Image acquisition was performed on an Opera QEHS (Perkin Elmer) with 45 plate capacity loader/stacker and the following settings:
- 40x 0.6 NA air objective
- Acquisition camera set to 2-by-2 binning for an image size of 688 by 512 pixels
- 2 channels acquired sequentially: Exp1Cam1 = Beta-arrestin GFP using 488 nm laser excitation and 540/70 nm mission filters, Exp2Cam2 = DAPI (nuclei) using 365 nm Xenon lamp excitation and 450/50 nm emission filters
- 4 fields per well for Hit Confirmation
2) Image analysis was performed using the Acapella Spot Detection Algorithm with the following analysis settings:
- Threshold Adjustment: 4
- Nuclear Splitting Adjustment: 10
- Minimum Nuclear Area: 200
- Minimum Nuclear Contrast: 0
- Cytoplasm Threshold Adjustment: 0
- Cytoplasm Individual Threshold Adjustment: 0.05
- Spot Minimum Distance 3
- Spot Peak Radius 0
- Spot Reference Radius 3
- Spot Minimum Contrast 0.26
- Spot Minimum to Cell Intensity 0.5
3) Metrics calculated from
NUCLEI IMAGES: Cell Count ("NumberofCellsAnalyzed"), Nuclei Area ("AreaoftheNucleus"), Integrated Intensity of the Nuclei ("TotalIntegratedIntensityoftheNucleus"), Average Intensity of the Nuclei ("AverageIntensityoftheNucleus")
GFP IMAGES: Integrated Intensity of the Cytoplasm ("TotalCytoplasmIntensity"), Integrated Intensity of the Detected Spots ("TotalSpotIntensity"), Ratio of the Integrated Spot to Integrated Cytoplasm Intensities ("RatioofSpotIntensitytoCytoplasmintensity"), Number of Spots per Cell ("AverageSpotsPerCell"), Percentage of Cells Positive for Spot Formation ("PercentagePositiveCells")
4) The "RatioofSpotIntensitytoCytoplasmintensity" metric was used to calculate the dose response curves and parameters.
IC50 values were calculated using CBIS software (ChemInnovations) employing a sigmoidal dose-response equation through non-linear regression.
Compounds with an IC50 < 10 uM are defined as actives in the dose response confirmation.
To simplify the distinction between the inactives of the primary screen and of the confirmatory screening stage, the Tiered Activity Scoring System was developed and implemented. Its utilization for the assay is described below.
Activity Scoring
Activity scoring rules were devised to take into consideration compound efficacy, its potential interference with the assay and the screening stage that the data was obtained. Details of the Scoring System will be published elsewhere. Briefly, the outline of the scoring system utilized for the assay is as follows:
1) First tier (0-40 range) is reserved for primary screening data and is not applicable to this assay
2) Second tier (41-80 range) is reserved for dose-response confirmation data and is not applicable to this assay
3) Third tier (81-100 range) is reserved for resynthesized true positives and their analogues.
a. Inactive compounds of the confirmatory stage are assigned a score value equal 81.
b. For the remaining compounds the score is linearly correlated with a compound's inhibitory potency and, in addition, provides a measure of the likelihood that the compound is not an artifact based on the available information.
c. Summary equation that takes into account the items discussed above is
Score = 82 + 3*(pIC50 - 3),
where pIC50 is a negative log(10) of the IC50 value expressed in mole/L concentration units. This equation results in the Score values above 85 for compounds that demonstrate high potency and predictable behavior. Compounds that are inactive in the assay or whose concentration-dependent behavior are likely to be an artifact of that assay will generally have lower Score values.
Categorized Comment - additional comments and annotations
From ChEMBL:
Assay Type: Functional
Result Definitions
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1X01 DA026205-01
Data Table (Concise)


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