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Promega Corporation
Performing PCR in real time means that product formation is measured at each cycle as it is occurring by measuring a change in a fluorescent reporter signal. With traditional, end-point PCR the signal is measured only once after the cycling program has ended. Real-time PCR should not be confused with RT-PCR, which is reverse transcription PCR, where the starting template is RNA that is reverse transcribed into a cDNA target prior to PCR.
There are two general types of fluorescent reporters that are used in real-time PCR assays:
Double-stranded DNA-binding Dyes
Double-stranded DNA-binding dyes are dyes that bind to the minor groove of double-stranded DNA (dsDNA). The free dye has a very low fluorescence, whereas the bound dye has a high fluorescence. The more double-stranded PCR product that is produced, the greater the fluorescence signal will be. BRYT Green® Dye is an example of a dsDNA-binding dye. These dyes allow you to use standard PCR primers, so there is no need to order special labeled primers. The disadvantage is that the signal is not sequence-specific, so you will need to verify that the reaction is producing the desired product.
Labeled Primer or Probe
The second type of reporter method uses a labeled primer or probe to detect the amplification product. There are many different ways to modify reporter signal generation. Real-time PCR using labeled oligonucleotide primers or probes employs two different fluorescent reporters and relies on energy transfer from one reporter (the energy donor) to a second reporter (the energy acceptor) when the reporters are in close proximity. The second reporter can be either a quencher or a fluor. If the second reporter is a quencher, the energy from the first reporter is absorbed but re-emitted as heat rather than light, leading to a decrease in fluorescent signal. Alternatively, if the second reporter is a fluor, the energy can be absorbed and re-emitted at another wavelength through fluorescent resonance energy transfer (FRET, reviewed in Didenko, 2001[2]), and the progress of the reaction can be monitored by the decrease in fluorescence of the energy donor or the increase in fluorescence of the energy acceptor.
Some qPCR strategies employ complementary nucleic acid probes to quantify the DNA target. These probes also can be used to detect single nucleotide polymorphisms
. There are many different types of real-time PCR probes, including hydrolysis, hairpin and simple hybridization probes. Probes contain a complementary sequence that allows the probe to anneal to the accumulating PCR product, but can differ in the number and location of the fluorescent reporters. The use of simple hybridization probes involves two labeled probes or, alternatively, one labeled probe and a labeled PCR primer. In the first approach, the energy emitted by the fluor on one probe is absorbed by a fluor on the second probe, which hybridizes nearby. In a second approach, the emitted energy is absorbed by a second fluor that is incorporated into the PCR product as part of the primer. Both of these approaches result in increased fluorescence of the energy acceptor and decreased fluorescence of the energy donor. The TaqMan® Assay is an example of a method that uses a labeled probe. The big advantage that label-based methods offer is the ability to multiplex two or more targets in a single reaction. This would allow you to measure an experimental gene and a control gene for normalization in the same reaction.
The primary output from your real-time PCR instrument is an amplification curve. The curve shows the fluorescent signal as RFUs versus the cycle number and thus shows the accumulation of amplified product. The amplification curve can be broken down into three parts. The baseline is the point early in the amplification process where very little product has been produced and the signal is still below the background noise of the assay. The exponential phase occurs around the time that the reaction reaches the amplification threshold; and indicates that the product is doubling with every cycle. This phase can be seen more clearly if the results are graphed on a semi-log plot.
The amplification threshold is calculated based on the background fluorescence over the baseline region of all the curves in the group. The threshold represents the point where the reporter signal is significantly higher than the background levels.
Quantifying the amount of starting material is done using the quantification cycle (Cq; also known as Ct). The Cq is the cycle number where the amplification curve crosses the amplification threshold. Once you have the Cq value, there are two approaches for converting that value to template quantity. In absolute quantification, the Cq value can be used to determine the amount of an unknown sample using a standard curve derived from reactions which contain known amounts of the target RNA (cDNA). The standard curve is generated by plotting the Cq versus concentration (log concentration) for the standard curve samples. The concentration of unknown samples then can be determined using an equation derived from the linear regression analysis of the standard curve.
Most real-time PCR instrument software can also perform relative quantification using the Cq values. Relative quantification is based on the premise that each template is replicated with each cycle; therefore, n cycles would produce 2n product. This relationship also works in reverse; for example, two samples with a Cq difference of 1 would have a twofold difference in starting template amount. This is the basis for the simplest algorithm for relative quantitation
; however, this is only accurate if amplification efficiency is 100 percent. Amplification efficiency can be calculated from a standard curve (done during assay validation) using the equation shown in Figure 1
. If amplification efficiency is less than 100%, then relative quantification calculations must be adjusted to correct for the decreased efficiency
RT-qPCR is the same as qPCR but using a starting template of RNA that is reverse transcribed (RT) to cDNA prior to amplification. A successful RT-qPCR assay is dependent on the success of the RT reaction, and successful reverse transcription depends on RNA integrity and purity. There are several methods for evaluating your RNA before proceeding to the RT step. RNA integrity can be assessed qualitatively by gel electrophoresis or quantitatively using systems such as the Agilent Bioanalyzer, which uses microfluidics to size‐separate and quantitate RNA. RNA quantity and purity can be assessed by absorbance. The quantity is determined by absorbance at A260, and quality is measured using a ratio of absorbance at A260/A280. High‐quality RNA should have a minimum A260/280 ratio of about 2. Spiking experiments, in which RNA of unknown quality is added to an RT-qPCR that has amplified well in the past, can be used to detect inhibitors. It is a good idea to include a no-RT qPCR control reaction to detect gDNA contamination. Finally, quantifying the RNA will help normalize the amount of RNA you are including in each reaction.
Anytime you are working with RNA, RNA degradation is a concern. We recommend that you have a dedicated, ribonuclease-free (RNase-free), area for all RNA work. Procedures for creating and maintaining a RNase-free environment to minimize RNA degradation should be followed faithfully. Using an RNase inhibitor (e.g., Recombinant RNasin® Ribonuclease Inhibitor) is strongly recommended.
The fundamental difference between these two approaches is whether the RT step is performed separately from the PCR or in the same tube. For one-step RT-PCR, the RT and PCR steps are performed sequentially in the same tube using the entire amount of the cDNA synthesis products as the template for PCR. For two-step RT-PCR, the RT and PCR steps are also performed sequentially, but only a portion of the cDNA products is used as a template for PCR, which is performed in a separate tube. For this reason, one-step RT-PCR may be the more sensitive approach. However, two-step RT-PCR allows multiple PCRs from a single RT reaction, which works well for quantifying multiple targets or for doing replicate assays.
One-step RT-PCR commonly uses gene-specific primers for both the RT and PCR steps, with one of the PCR primers also acting as the RT primer. Two-step RT-qPCR can use oligo(dT) primers or random primers for the RT step. For RT-qPCR, a sequence-specific primer also can be used.
ΔR: baseline-corrected fluorescence (R)— R minus average R over baseline region.
Passive reference: free dye (unassociated with amplification product) used for normalization to control for experimental and optical variation).
Rn: normalized fluorescence — R divided by passive reference.
ΔRn: baseline-corrected, normalized fluorescence.
Experimental Target: the target gene, transcript, channel or dye of interest.
Control Target: normalized, endogenous control, reference gene, transcript, channel, dye or housekeeping gene.
Experimental Sample: unknown or treated sample.
Control Sample: calibrator, reference sample.
MIQE: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
MIQE provides authors, reviewers and editors specifications for the minimum information that must be reported for a qPCR experiment to ensure relevance, accuracy, correct interpretation and reproducibility. A checklist, which should be submitted along with the paper, is available for authors preparing a manuscript employing qPCR.
"Maximize Your Reverse Transcription-qPCR (RT-qPCR) Assays" Dr. Carl Strayer
RNA Extraction and Quality Control
"Working with RNA"
"RNase Contamination Happens; Recombinant RNasin® Inhibitor Can Safeguard Your Samples."
"RNA Purification Kit Comparison: Yield, Quality and Real-Time RT-PCR Performance"
Gene Quantification: www.gene-quantification.info/
A–Z of quantitative PCR (Editor: S.A. Bustin ), International University Line (IUL), La Jolla, CA, USA.
Design, Validation, Controls, Normalization
Sean Taylor, "A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data" 2011, Bio-Rad tech note 6245.
Garbarino-Pico, E. et al. (2007) Immediate early response of the circadian polyA ribonuclease nocturnin to two extracellular stimuli. RNA 13, 745–755. PMID:17400819
Pre Designed qPCR Primers
Primer Bank
Primer Design Software
Scientific Style and Format, 7th edition, 2006
Promega Corporation
FAQ for RT-qPCR.
[Internet] 2012. [cited: year, month, date]. Available from:
American Medical Association, Manual of Style, 10th edition, 2007
Promega Corporation
FAQ for RT-qPCR.
Promega Corporation Web site.
Updated 2012.
Accessed Month Day, Year.
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.


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For New York Fashion Week, we pulled together seven fashion brands that drove denim to new levels and offered inspiration on what should be made in denim. We were so in love with some pieces that were not quite denim, that we threw those photos in for your ogling. We think you'll forgive us.
Band of Outsiders—We've known for a while that the fashion houses have been fixated on Great Depression and Dustbowl era fashion, and there's still a bit of this here, but with a mix of glam and luxury fabrics (we count suede and fur collars), there's a sense that the sun-kissed models in workwear-inspired denim jumpsuits and white-collar attire, offers hope that there's a light at the end of the tunnel. Images via Style.com.
Diesel Black Gold—From red to blue denim, not only has Diesel gone gaga over color, they've gone Jackson Pollock with their side-tie pants and cropped jeans. With a definite homage to an 80′s fashion trend, the paint splashes and printed jeans serve as a contradiction to their progressive leatherwear. We couldn't escape the sense of a Mad Max meets Highlander effect when everything was pulled together. Images via Style.com.
Concept Korea (V)—In an effort to bring visibility to South Korea's top fashion labels, this collective was born. In it's second year at NYFW, Concept features five top Korean labels. Most notably, the design duo Steve Jung and Yoni Pai (of the brand Steve J & Yoni P) with collaborations between Topshop and 10 Corso Como (Seoul) under their belt, they make denim part of their repertoire. Images via WWD and Mercedes-Benz Fashion Week online.
Kelly Wearstler—As if to defy anybody who had any reservations about 80s fashions, this brand has committed wholeheartedly to the styling of acid wash jeans and stirrup leggings. Even if the ultra high-waisted acid wash zipper skirts aren't your thing, you might just have a crush on the jackets. Images via Style.com.
Need more fashion week fix? Read our coverage of Levi's Fall 2012 and what denim heads wore at NYFW.
—Kathy Ng Hassan
Tags: Band of Outsiders, Concept Korea, Diesel Black Gold, fall 2012, Gilded Age, Kelly Wearstler, New York Fashion Week, Nicholas K, nyfw, rebecca minkoff, Steve J and Yoni P

subset: commoncrawl


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Dave & Gene's – Watch & Learn
Dave & Gene's Flooring America is the ONLY Authorized Stainmaster Flooring Center within Hundreds of miles of Sioux Falls...
Dave & Gene's Flooring America is the ONLY Authorized Stainmaster Flooring Center within Hundreds of miles of Sioux Falls...
"ANY Room - ANY Floor - ANY Budget"
These guys love floors. They love helping people find the right floor for any room. That's why these two brothers, Dave & Gene opened a better floor covering store and put their names on it. Dave & Gene's Flooring America
Sioux Falls HVAC Contractors Foley's Heating & Air have been taming temperatures and coordinating comfort in homes and businesses since 2003.
They install, service, repair and sell heating and cooling systems for homes and small businesses.
Howe Electricians are experienced in all aspects of the electrical field and no problem is too big or too small! Read on for a list and description of services and offers.
Residential: If you need electrical work inside or outside of your home…if you're remodeling, or building new, or just need to
Howe Electric has been in Sioux Falls since 1973!
Watch and listen to hear more of what Howe Electric can do, have their Pickle Nickel guarantee explained, and get questions like these answered:
Rew's TV & Appliance believes people deserve to be taken care of. They have been around since 1961 building trust and providing a needed service to south eastern South Dakota.
Started by Art Rew, Dan Rew now continues that tradition along with 14 qualified, experienced, and good people.
Would you like your concrete to come alive at NIGHT?
Take a look at the new Casper Stone that glows in the dark! So many ideas…so little time. You have to see it to believe it! Visit brooksconstructionservices.com to see som


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Practice Philosophy
Aesthetic surgery is neither form alone nor function alone, but rather a natural and harmonious blending of the two. Our philosophy is simple-we are a high-end aesthetic practice and no effort is spared to provide our patients with the very best in all aspects of their care. We believe in a "less-is-more" philosophy - just as the skilled writer or artist conveys their message with the fewest, most carefully chosen words or brush strokes, we help our patients achieve their aesthetic goals with the fewest, least invasive, safest and least costly techniques. Most procedures are performed in our accredited office facility without need for general anesthesia. We carefully evaluate and employ recent technological developments in our field (laser, ultrasonic liposuction, etc.) and we combine them with time-proven techniques to optimize our patients' results. We strive to minimize the recovery period and we understand that it is difficult for our patients to miss more than one week from their professional and social schedules.
Aesthetic Surgery
Facial Rejuvenation
Liposuction/body contouring
Breast Augmentation/reduction
Laser Surgery and Eyelid Surgery
pre- and post-operative photographs, are available on our web site at www.plasticsurgeryct.com.
Approximately 45 minutes to one hour is allotted for an aesthetic consultation and most patients are seen twice prior to surgery. A comprehensive medical history is taken and we will work with your other physicians to coordinate your care and minimize risk. Most procedures are performed in our accredited in-office surgical facility without need for general anesthesia. All patients meet with our board certified anesthesiologists prior to surgery. For those with medical problems or patients who require an overnight hospital stay, we offer affordable rates in conjunction with a local hospital. Local hotel accomodations, limousine service, a private entrance and two waiting areas are also available.
During your consultation we will review your aesthetic goals and perform a careful examination. We will discuss in great detail the various surgical and non-surgical options (as well as their potential benefits and risks) and we will together formulate a plan designed to address your goals. Computer imaging and photographs and take-home written materials are educational tools offered to our patients.
In facial rejuvenation, we strive for a rested, natural, harmonious, non-surgical look. Most eyelid surgery is performed under only local anesthesia. Dr. O'Connell has published articles on the use of laser resurfacing with more traditional facelift techniques and he is experienced with the new "short-scar" or "minimal incision" facelifts. Dr. O'Connell was the first plastic surgeon in Connecticut to perform ultrasonic liposuction and we assist our patients in the choice of the safest and most effective liposculpture techniques. Breast augmentation patients are encouraged to "try-on" implants for size and we believe the aesthetics of breast shape are equally important to the aesthetics of size. Dr. O'Connell has published two articles on breast reduction and, as with abdominoplasty and other aesthetic procedures, we strive to minimize the length of your surgery, anesthesia and recovery time. Dr. O'Connell performs the so-called "scarless" or "liposuction only breast reduction" procedure in his office facility without need for general anesthesia. In 2005 Dr. O'Connell performed a blepharoplasty under only local anesthesia for the CBS Early Show and this may be viewed on YouTube.
Plastic Surgery of Southern Connecticut, L.L.C. includes a state-of-the-art outpatient surgical facility that has been both licensed by the State of Connecticut and accredited by the American Association for Accreditation of Ambulatory Surgical Facilities, Inc. (AAAASF) as a Level C (the highest level) facility. Anesthesia is administered by board certified anesthesiologists. Our on-site Medical-Spa, Skin Plus Medical Spa, includes a laser facility for the treatment of unwanted hair, blood vessels and wrinkles. Photorejuvenation with intense pulsed light, as well as microdermabrasion, medical skin care and photodynamic therapy for acne are also offered.Â
Location & Office Hours
208 Post Road West
Westport, CT 06880
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Location #2
Plastic Surgery of Southern Connecticut, L.L.C.
208 Post Rd. West
Westport, CT 06880
View Map
Payment Options
American Express, Discover, Visa, MasterCard, Patient Financing, Cash, Personal Checks, CareCredit


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July 3
Wednesday, 11:00 am - 12:00 pm
"Summer Wonder" Event
Native Nations Dance Theater
Penn Museum's Summer Wonder program, appropriate for guests of all ages and their families, kicks off with this interactive performance. Native Nations Dance Theater invites you to "keep the Native Spirit alive." Celebrate Native American culture through song, dance, storytelling, and traditional regalia. Witness the Round Dance, Snake Dance, and more. Help drum out some rhythms at your seat or join presenters on stage to try some dance moves. All Summer Wonder performances are free with Museum admission.
Read about the full series of Summer Wonder events here.
3260 South Street
Philadelphia, PA 19104
(215) 898-4000
Tuesday-Sunday: 10:00am - 5:00pm
First Wednesdays: 10:00am - 8:00pm
Monday: CLOSED


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High School Valentine's Day Activities
Valentine's Day activities get your child to lovingly create cards, snacks, and crafts. This heart-filled February holiday is a fun time to celebrate love and friendship with your child. Your little crafter can have fun preparing for February 14 by doing Valentine's Day activities. Many of these Valentine's Day activities make nice presents that your child can gift to friends and family members. Sip some raspberry hot chocolate with your child as you make a melted crayon heart, plate a Valentine's Day salad, and construct a red-and-pink mailbox. Bring some love to your craft table with Valentine's Day Activities.
Valentine's Day


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Joseph Forgotten?
Isaiah 49:15 tells us that the unthinkable may even happen at times — a mother forgets her nursing baby. But God will never forget His people. Even when we do not see His helping hand, the Lord remembers us in ...Read More
From Jacob to Israel
When God sends trials our way, He is not working against us. He also delivers us from the travails He permits (Pss. 18:2; 74; 79; 1 Cor. 10:13). Calvin writes that "inasmuch as he supplies us with more strength to ...Read More
Be Not Surprised
Biologically speaking, Cain and Abel were from the same family. However, they did not share the same spiritual heritage. Though we might think they were brothers, in an ultimate and eternal sense they were not. The church can also be ...Read More
Called to Suffer
Take some time today to read through 1 Peter and consider the call to stand firm for Jesus even when it brings suffering to us. Spend some time in prayer meditating on how willing you are to bear the sufferings ...Read More
A Divine Vocation
Lest we think that Christ bore His pain with ease, let us remember that even Jesus asked for the cup of suffering to pass from Him (Luke 22:39–42). Yet He did this in faith, submitting to the Father's will when ...Read More
Surprised by Suffering
When we come to the issue of suffering, we can see how God's sovereignty is a comfort to us. Because He is in full control of all events past, present, and future, we know that ultimately there can be no ...Read More
Be Not Ashamed
John Calvin writes, "it is no common kindness from God, that he calls us, freed and exempted from the common punishment of our sins, to so honourable a warfare as to undergo for the testimony of his Gospel either exiles, ...Read More
Deserved Suffering
How apt are you to play the part of the righteous victim? Do you become indignant when you experience hardship even though you might deserve it for meddling in someone else's affairs? Though we are called to rescue others from ...Read More
The Spirit of Glory
Peter reminds us today that if we suffer for bearing the name of Christ we are blessed. Just as the Holy Spirit rested upon Jesus, so too does He rest upon us, and our suffering proves that we indeed have ...Read More
Arm Yourselves
When Peter says that those who suffer in the flesh have ceased from sin, he is not teaching that sinless perfection is possible in this life. Until we are glorified we will still sin. However, as John Calvin writes, if ...Read More
DevotionalsDaily readings to encourage your heart and mind in the pursuit of God and his glory.
- All
- Angels and Demons
- Atonement
- Basic Reformed Theology
- Irresistible Grace
- Limited Atonement
- Perseverance of the Saints
- Total Depravity
- Unconditional Election
- The Church
- Church Discipline
- Church Leadership
- Creeds and Confessions
- God
- Holiness
- Sovereignty
- Heaven and Hell
- Heresies
- The Holy Spirit
- Jesus Christ
- Cross
- Incarnation
- Resurrection
- Justification
- Last Things
- Man and Sin
- Sacraments
- Salvation
- Faith
- Regeneration
- Scripture
- Systematic Theology
- Theological Views
- Roman Catholicism
- Music
- Apologetics
- False Teaching
- Atheism
- Christianity and Culture
- Creation
- Ethics
- Fiction and Literature
- Philosophy
- Science
- Bible Figures
- Bible Interpretation
- Bibles
- Books of the Bible
- Gospels
- Covenants
- General Reference Works
- Introduction to the Bible
- Jesus' Ministry
- Life of Jesus
- New Testament
- Old Testament
- Parables
- Prophecy
- Sermon on the Mount
- Ten Commandments
- The Lord's Prayer
- Controversies in the Church
- Arts
- Bible Translation
- Covenant Theology
- Creationism
- Education
- Inclusivism
- Law and Gospel
- Lordship Salvation
- Politics
- Sabbath
- Spiritual Gifts
- Stewardship of Creation
- Theonomy
- Women in Ministry
- Marriage and Family
- Divorce
- Parenting
- Practical Theology
- Discipleship
- Evangelism and Missions
- Grief and Death
- Pastoral Ministry
- Preaching
- Worship
- Spiritual Growth
- Anxiety and Worry
- Apostasy
- Assurance
- Conscience
- Depression
- Doubt
- Fellowship
- Godliness
- Guilt
- Humility
- Joy
- Knowing God's Will
- Legalism
- Love
- Money and Stewardship
- Oaths and Vows
- Prayer
- Pride
- Repentance and Forgiveness
- Sanctification and Growth
- Service
- Spiritual Fruit
- Suffering
- Thankfulness
- Vocation
- Ancient Church
- Biography
- Enlightenment and Modernism
- Historical Theology
- Reformation
- Twentieth Century


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Freedom Flix
FOOD FIGHT IN WASHINGTON: Let's Save DC Food Trucks
D.C. is one of the best food-truck cities in the country. That's something city officials should be proud of. But unfortunately, city leaders are considering new restrictions on food trucks that could be used to ban them from serving their customers in many parts of the city. If passed, these restrictions would make D.C. one of the worst cities for food trucks. In addition to reducing options for customers, these restrictions could force many food trucks to lay off employees, relocate to other cities with better laws, or go out of business altogether.
Let's work together to Save DC Food Trucks!Read more
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Freedom Flix
The Institute for Justice is always looking for new ways to promote the message of freedom. To that end, IJ produced the following videos in-house to tell the stories of our clients and their fight for individual liberty.
None of this — the cases or these videos — would be possible without the continued generosity of our donors. We hope you enjoy them and share them with those who need a little inspiration.
Subscribe to our videos via iTunes or YouTube.


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AKRON -- The Akron Police Department, in a collaborative effort with the Summit County Office of Consumer Affairs, would like to offer the following tips to avoid being scammed this Valentine's Day:
Social Media Sites: Use caution with links that look as if they were sent by a friend.When you click on that link, you may unknowingly share your information with scammers ...who might post on your page or send their information to all of your contacts. It is recommended that you use the strongest privacy settings.
Flower Orders: If you receive an email or telephone call explaining that the flowers you ordered for your sweetie will not be delivered unless you give your credit card number, it is a scam. Con-artists send hundreds of emails, and are hopeful that a few messages will reach consumers who really have sent flowers. If you think the message is real, first call the florist or go directly to the florist's website.
Email cards: Be careful opening a Valentine's Day eCard. It could actually be a phishing scheme to obtain your personal information or to infect your computer with spyware. Do not open non-personal emails, e.g. "from your secret admirer." Only open email from people you know and trust. Update firewalls and anti-virus software on your computer or if none, install them.
Online Dating: Proceed with caution after meeting the perfect mate. Scam artists often create fake profiles to lure vulnerable people into revealing personal information, or to ask for money because they are sick or have a relative who needs emergency medical attention.Be wary of free dating sites as they may offer less monitoring and protection than those that charge a fee.
Ordering Gifts over the Internet: Be careful of pop ups offering discounts. Hidden in that pop-up is fine print that tells you that you have agreed to join a fee-based membership program. You will find small recurring charges on your credit card or bank account.
If you experience unauthorized charges, please contact the Akron Police Department, Financial Crimes Unit, at 330-375-2600, or the Office of Consumer Affairs at 330-643-2879, to file a complaint.


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UCSF Medical Center is among the nation's premier hospitals for the 14th consecutive year — ranking as the eighth best hospital in the country in the 2015-2016 America's Best Hospitals survey.
As part of a collaboration with CMPC, a total of 18 donors and recipients underwent surgery in a little more than 36 hours, with nine people donating and nine receiving new kidneys.
Watch Dr. Diane Sliwka discuss her work helping doctors communicate better — so even when there's a sad outcome, patients and their families can feel good about their care.
A grisly accident changed Miguel Castellanos' life forever, but through evolving technology and his own perseverance, he's challenging the limits of what's possible.
UCSF Benioff Children's Hospitals are here to help every child have the chance to star in whatever their story will be.


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This story was updated on Jan. 25, 2013.
Representatives from about 20 Maryland businesses were among the 500-plus people who flooded an assembly room at the General Services Administration headquarters in Washington, D.C., one afternoon last week, all with their eyes on one prize: landing the FBI's new headquarters.
But some also questioned the feasibility of the GSA's hopes of exchanging the outdated J. Edgar Hoover Building in downtown Washington with developers of the new campus.
The crowd, including business executives and officials from Montgomery and Prince George's counties, faced long wait lines and was often shuffled into an overflow room, as GSA officials dealt with greater interest than they had expected, county officials said.
Last month, the GSA released a request for information from the business community for developing a new 2.1 million-square-foot FBI headquarters within 2.5 miles of the Capital Beltway. The nation's chief law enforcement agency is now in the Hoover building. The new complex would cost billions and employ 11,000 workers, plus contractors.
Peter Brooks of DOO Consulting was at the Jan. 17 event — dubbed FBI Headquarters Industry Day — to explore opportunities for his sustainability consulting company. Brooks referred to DOO, with offices in Bethesda and Baltimore, as the "H&R Block of the U.S. Green Building Council."
Brooks, director of business development, said the event helped him understand the GSA is interested in Leadership in Energy and Environmental Design certification from the Green Building Council for this project, something that was not clear in the request for information, he said.
"I left the event excited and very inspired," he said, adding that while he wishes his company had time to show the GSA a full range of innovative sustainability options, it probably has time only to speak about the "virtues" of hiring a sustainability consultant.
The GSA wants information responses by March 4.
Brooks also lamented that businesspeople couldn't really network at the gathering because of its crowded setup, although he appreciated the chance to meet with the officials involved. He recommended the Washington Convention Center for future presentations.
While John Voycik, senior principal with Laurel civil engineering firm Stantec — formerly Greenhorne & O'Mara — called the event worthwhile for his company, he also is skeptical about the GSA's aspirations. The GSA wants to swap the Hoover building with developers for the new campus, should a site be selected.
"I don't think they're being realistic about what the development community can do here," Voycik said, adding that the Hoover building will probably cost developers significantly for renovations. "I don't see a one-for-one swap."
Voycik said he attended the event because the former Greenhorne & O'Mara has long been involved in high-profile, large federal consolidation projects and has the experience from an environmental engineering perspective to support the GSA and architects in this project.
The GSA might have to look for other ways to make this project happen, given the fiscal constraints on both the federal government and the development community, he said, and nothing major will likely happen with the project until late in the year at the earliest, he said.
On the local government side, the event highlighted the fierce competition involved in this process, said David Iannucci, assistant deputy chief administrative officer for economic development and public infrastructure for Prince George's.
"We emerged with a renewed sense of confidence that we have the superior sites and can make the private partnerships to see this through," he said, adding that Northern Virginia counties also are very interested.
Prince George's has a good advantage with its available acreage, access to federal highways and public transit and available developments with the business community support for such a project, Iannucci said.
"We're virtually convinced Montgomery County doesn't have any sites that meet those requirements. Virginia is focusing on the Franconia-Springfield area," he said. "Virginia is always going to be strong competition.
"Prince George's is all in on going after the FBI headquarters," Iannucci said, adding that the county will work with elected officials at all levels to make a strong case.
Tina Benjamin, manager of special projects for the Montgomery County Department of Economic Development, said Montgomery does not have properties that could meet the requirements, and she could not think of any specific sites that hit the exact standards, but she said the private sector might have ideas.
"There are hundreds upon hundreds of companies interested in this project. I arrived 10 minutes early and waited 20 minutes to get inside," she said. "It was ridiculous. It's a huge real estate deal."
She also said GSA officials did not focus much on the request for information during the event and mainly focused on the deals it could make for redeveloping the area around the Hoover building, a prize commercial and residential neighborhood.


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Inconspicuous and multifunctional, the inventive accents by Sancal are the ingredients for a smart yet stylish abode. This patterned Cairo Pillow was created by Karim Rashid to accompany his Float Sofa, but will add a playful punch to any seat. Inspired by Egypt, the designer's birthplace, the cotton-covered cushion articulates his cheery childhood memories through vibrant, geometric shapes.
Removable cotton cushion cover. Filled with feather-touch microfiber.
This product has no reviews yet. Be the first to review this product. Write a review
Enjoy 10% off your first order*


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Contact Us 800-235-0289 Mon, Wed, Fri 9-5:30 / Tue, Thu 9-7 ESTFREE CATALOG
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The Community of Disc Golfers and About All Things Disc Golf
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Played a few days ago in snow over 1 foot deep - good exercise. We need a disc golf biathlon - cross country skiis or snow shoes and disc golf !
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Welcome to the RinkTime profile for USA's Skateplex Roller/Inline Skating Rink. Founded in Temple Terrace, FL, USA's Skateplex is an indoor rink and open year round.
Public skating sessions are one of our main attractions at USA's Skateplex. Skating provides great exercise and an opportunity to get out of the house - plus, it's not like exercising at the gym... skating is fun! Lace-em up and come join the others. Get on the floor and get ready for a great experience.
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Hi all, just wanted to post up this little pedestal I made for my bench vise. When I was in school at wyotech they had the vises mounted on old 18-wheeler rims so you could move them through the shop. I though that would come in handy since I'm working in a small 2-car garage. So one of the guys I work with was able to find me an old wheel and I bought the piece of plate and the 2x4 tubing and the rest is history. Let me know what you think. And yes, I know I'm using a Lincoln welder. Hoping to have my Miller TIG by the end of the year though.
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Thread: Bench vise pedestal
04-28-2010, 10:48 AM #1Junior Member
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Bench vise pedestal
04-28-2010, 03:38 PM #2Junior Member
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nicely done.
When you really need a vice, you need it bolted down to something that will not move. Your wheel will work for a lot if instances, but when you need to hold something in the vice and really crank on it, look for a way to mount that wheel to your concrete floor.Miller Syncrowave 200
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04-28-2010, 08:02 PM #4Junior Member
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It also works better if the vise is bolted to the stand.MM250
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04-28-2010, 08:59 PM #6Junior Member
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thanks for the compilents. If I really need to crank on something, the vise will get bolted to the bench. I just made this incase I have something really long that I need to work on or something that I may need to grind on while in the vise. Easier to do that stuff in the driveway. And yes, the vise is bolted to the stand now. I took those pics right after I finished making it.


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Friday, September 15, 2006
With players spanning nearly six decades, picking the best five of all time will not be an easy task, but fans can accept the challenge by voting for their favorites at ETSUBucs.com. Voting will continue until Oct. 1.
During the upcoming men's basketball season, the ETSU Department of Intercollegiate Athletics, the Pirate Club Scholarship Fund, and Phil Bachman Toyota/Scion have teamed up to honor these Top 5 players with special lapel pins. Each pin will be made to look like the replica jerseys for each player, and the first 2,000 fans to arrive for five selected home matchups will receive a different pin each game.
"ETSU has had a rich tradition in basketball and we thought it would be a good time to honor the very best to have donned the Blue and Gold," said ETSU Director of Marketing and Outside
Giveaway dates for the pin promotion are listed below.
11/15: Pin #1 - ETSU vs.
1/4: Pin #2 - ETSU vs. Lipscomb
1/13: Pin #3 - ETSU vs.
1/19: Pin #4 - ETSU vs.
2/2: Pin #5 - ETSU vs. Gardner-Webb
Top 15 All-Time Greatest Pin Nominees
Greg Dennis, 1987-92
2,204 points (2nd), 895 rebounds (2nd), 174 blocks (2nd)
Keith "Mister"
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Tom Chilton, 1959-61
1,801 points (5th), 26.10 points per game (1st)
Troy Lee Mikell, 1980-83
1,684 points (6th), 55 % FG percentage (7th), 637 FG made (5th)
2,300 points (1st), 508 assists (2nd), 313 steals (2nd)
Zakee Wadood, 2000-2004
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Harley "Skeeter" Swift, 1967-69
1,367 points (8th), 17.99 points per game (4th), 289 free throws made (8th)
Tommy Woods, 1965-67
1,034 rebounds (1st), 16.2 rebounds per game (1st), 15.30 points per game (11th)
Mike Kretzer, 1967-70
1,289 points (10th), 17.19 points per game (6th)
Greg Stephens, 1997-99
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Rodney English, 1990-92
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Herb Weaver, 1953-56
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- Mitch SMith
- Katoomba
- Australia
This conversation is closed.
What do we trade-off for the comfort of technology?
We all go for it - the quick-fix.
But later, we often find that our ignorance has been exploited for the advantage of someone else .. who's only virtue was to get there first.
Evolution has nothing to say about getting there first .. there's nothing there to code in genes.
So .. what I'm asking is - what are we giving up for comfort?
How much comfort is constructive, and at what point is it exploitive at the benefit of comfort for a few, but at the detriment to our species?
Closing Statement from Mitch SMith
The conversation went quite wide.
I am disappointed that few actually got the point of what advantage is traded off for comfort - and who gets it.
There are even those so vein to think that they are in total control of their lives.
Hey ho - at least we explored it a bit.
Jul 1 2013: We loss touch with the natural world and the processes taking place that are suppose to define our humanity. Science has given us some understanding of those processes but we are not using that understanding enough to rethink traditional values and remold our society to fit this evolving knowledge but instead we are using it to create toys to perpetuate this failed ideology of wanting more and better.
Jul 3 2013: Yep.
I get the feeling that we cannot detect the diminished returns of any specific comfort.
We presume that we are intelligent and that we should know better .. and our laws often reflect that assumption.
Do you ever get that insight that common-sense is not common at all? That these things we call common-sense are only known in retrospect?
More and more, I am perceiving the limits of humans .. maybe because I'm getting older, but a friend of mine once said:
"my life is like a table-top .. after a while, if I push something onto it, something else drops off"
Are humans making a mistake to think that we all have infinite "table-tops"?Jul 3 2013: Prosthetic limbs, cochlear implants, artificial hearts, health information technologies, water purifiers, solar panels, geo thermal energy, vertical farming, ocular implants, medical/assentive robots, electromagnetic implants for pain management, and more.
Do you just picture iPhones and xboxes when thinking about new technology?
Developing toys gets people to invest in technology they don't see a use for otherwise. Then we use the interest to create a feedback loop of innovation.Jul 10 2013: Hi Mitch:>)
In my perception, you answered the question in one of your comments on this thread....
" something to do with how humans balance....."
Technology is a tool, which we, as humans have created, and we can use the tools in many different ways. In medicine, for example, we have the technology to diagnose injuries and dis-ease, so we may know more about what we are dealing with for the purpose of staying healthy and/or healing.
While many older practices were abandoned, in favor of using only modern technology, we are now seeing medical professionals re-evaluating the benefits of some older practices....meditation, yoga, acupuncture, reflexology, iridology, etc. Some medical professionals are using everything that is available to support or treat the body/mind in our quest for health and healing, which I wholeheartedly support. This, to me represents a new open-mindedness, which I think is beneficial to humans.
There are some comments on this thread about media and communication systems. Those systems all have an on/off button which we can freely use....it is a choice. We can spend time watching tv, or using the computer mindlessly, and/or, we can benefit from the technology to learn, grow and evolve in a balanced way.
No one can exploit us with technology, unless we allow that to happen. As thinking, feeling, intelligent, multi-sensory, multi dimensional human beings, we have choices. Personally, It feels more comfortable when I am balanced, so I strive for that feeling.
Jul 10 2013: Hi Colleen - long time - great to hear from you!
On-off is not really true. we are addicted. And there is no "off".
I have practiced this in the past . how to destroy the off button and get whatever I wanted - and I got a lot.
I don't do that anymore .. it was just the game I was taught to play .. it's so boring after the first few times.
You have no will - people like me will over-ride it before you even know you are at risk.
There is another way.
Those alive cannot be owned or over-ridden. I truly hope you are in that space because I have a certain regard for you - and my people should get the best of me and nothing less. I have certainly gotten as much - so there's nothing to lose any more.
My sister is a teaching nurse .. she does research and practice of wound treatment with maggots. She has a colleague who researches leaches. And she is a leading expert on the techniques of pain management. None of this technology is new. It's little more than folk lore millennia old.
myself .. well, I don't get dental pain because melaluka alternifora gum leaves are keyed to the same bacteria that likes to infect teeth and these leaves grow around here.
If you have an open wound, it's best to put a piece of cloth an an ant nest overnight and apply the cloth to the wound next day - ants produce a broad spectrum antibiotic that they secrete around the nest.
If you have a fungal infection, honey is effective where no commercial treatment works.
Technology as we know it is a con-job.
tribal observation preserved through the culture is far more effective.
We are not commodoties - our surplus is for our culture, not for rich manipulators.
Our suffering is not for sale. And our sadness is as real as our happiness - to deny this is to fail.
Jul 10 2013: OK Mitch, if you feel addicted, that's your thing. I prefer to believe and practice "choice". I do not agree Mitch that I have no will, or that anyone can over-ride my choices. That idea seems to simply reinforce the idea that we have no choice, and I don't think that is an idea worth spreading. I like to support people (including myself) with the idea that we DO have choices:>)
I realize there is a new interest and research with the use of maggots/leaches, and it makes perfect sense, because maggots/leaches only feed on decayed material, so they actually clean the infected area, while encouraging increased blood flow, which is also good for healing. I'm not sure I would use it.....never know!
I found out about honey as a treatment, years ago when I was in Egypt, because I had an infection in the hand. When I went into a restaurant, the waiters "treated" my hand with honey:>)
When my daughter was tiny (about 6 months old), she got a bad cold. Her grandmother made a poultice with warm garlic and onions, and wrapped the baby's chest. Cold symptoms were gone very quickly. This serves the same purpose as Vicks Vaporub.....remember that product? I LOVE it, because that's what my mother used for chest congestion....apparently, she traded the garlic/onion poultice for the "new" Vicks! I don't think they sell it anymore, but I still have an old jar of it:>) Modern medical professionals sometimes want to give us a pill rather than the remedies that may have worked really well...we have a choice in my humble perception:>)
I grow and use lots of herbs, which are both medicinal and very enjoyable to eat and drink in various forms:>) It looks like you have a new interest in Shamanism? GREAT!
Jul 12 2013: Hi Colleen,
Addiction isn't always negative .. a large amount of human value is generated by people accommodating their quirks and foibles into productive pursuits .. a psych professor once told me that hyper-productivity can be one outcome of trauma etc .. so it is never so cut and dried.
I am glad you are not available to manipulation - I have seen it practiced, and it gets a bit scary when you see how choice can be less than we assume. Most of this stuff gets practiced as a bag-of-tricks" for personal advantage, so I don't have much respect for it - better to understand the principles and gain a better responsibility as a result.
Scientific medicine has given us much .. penicillin is the prime example, but there is something a bit more robust with folk lore - it's intergenerational in a way that science cannot support .. science tends to become shrouded in enclaves of academia and patent laws. Apart from that, I like the idea of being in a family and tradition that can give from the heart .. not just a matter of pride, but of feeling of value and integrated.
P.S. shamanism took an interest in me .. a while ago. I don't talk about it often because there's not much of it that corresponds to the way it is represented .. we get images of witch doctors and formulaic ritual .. it's not like that at all .. well, not for me. Objects can have a certain power, but the crafting of them conforms more to circumstance without any ruleset .. it is a craft without precedent and cannot be taught as some assume .. not beyond a very general sense of "there's something over there". Talking with animals is the same .. they have something to say, or not, and one has to be in the right "phase" and time .. confluences that cannot be contrived .. happen .. or not. Acceptance is the only truth of it - it is not available to proof or disproof .. beyond that .. well, can observe things that deliver one-off advantage, but never for the practitioner..Jul 13 2013: I agree Mitch, that addiction isn't always negative, and I didn't say that it is. I have wholeheartedly admitted to having an addiction to gardening and TED:>)
You wrote..."You have no will - people like me will over-ride it before you even know you are at risk."
I don't agree with that statement, because I know I have choices, just like everyone has choices. That is why I don't perceive any " trade-off for the comfort of technology" in my life. I use technology as a tool, and I know I have choices regarding how I balance the life experience. In my perception, nothing is ""cut and dried" my friend:>)
If we don't truly believe that we have a choice, then yes, it is easier for others to manipulate us. If we "know" our self, and know that we have choices, it is less likely that we can be manipulated. As you insightfully say..." better to understand the principles and gain a better responsibility...". If we don't make plans for ourselves, somebody else will! LOL:>)
Funny how that often happens.....someone/something takes an interest in US! I have the same feeling at times.....interests/activities call to me, and create excitement about learning. I had a little background for some of the concepts of shamanism because I've grown and used herbs for a long time, have been very interested in the body/mind connections in regard to healing, and have explored holistic healing.
Years ago, I met a person who had lived and studied with a Shaman in So. America for a year. We became good friends, and of course he shared much of what he had learned. At the same time, I got lots of books about Shamanism and explored/studied on my own:>)
Fritzie -
Jun 30 2013: You may find interesting this year's research in New York, Berlin, and Mumbai of the BMW Guggenheim Lab, a traveling urban think tank.
- +1
Their theme for this first year has been "Confronting Comfort." http://www.bmwguggenheimlab.org/what-is-the-lab/theme
Their work in New York most clearly focused on this theme, while the work in Mumbai revisited in a slightly different way by focusing on privacy as a dimension of comfort. The lab's work in Berlin strayed a bit from this theme, focusing mostly on the lack of communication between the government and urban population in determining uses of urban public spaces but more centrally private dwellings, as gentrification takes hold there.
Comment deleted
Jun 30 2013: Did you follow the DIY projects, particularly in Berlin?Jun 30 2013: The Lab is specifically urban, so no, I have not seen anything from them with a rural focus. The same is true of the Sustainable Cities Collective.
The Berlin Lab had a little featured design of a sort of box shelter that could go anywhere and that lots of people made side by side as part of the lab's DIY stuff, but it is only a shelter- without any sort of facilities.
At this point every home show displays little houses built within shed-like structures. Even IKEA shows an interior.
But I fear we are getting somewhat away from Mitch's question.Jul 1 2013: Are you familiar with an organization called Architecture for Humanity? They are specialists in very low cost shelter, primarily of the type that can be erected pretty quickly and at low cost in emergency situations.Jul 1 2013: It's not that far off topic .. given that the topic is incredibly broad - navigation from the periphery to the core could reveal some important factors.
With the accelerated urbanisation of humanity .. I get reminded of the invention of agriculture, and how it precipitated sedentary lifestyles.
My personal policy about cities is "They are a great place to make money - but have an escape strategy."Jul 9 2013: Here is a poetic response from the BMW- Guggenheim Lab: http://blog.bmwguggenheimlab.org/2013/07/100-urban-trends-confronting-comfort/?utm_source=feedburner&utm_medium=email&utm_campaign=Feed%3A+bmwguggenheimlab%2Flog+%28BMW+Guggenheim+LAB+%7C+log%29&utm_content=Yahoo%21+Mail
Jul 10 2013: Yep.
Thanks for this.
Straight away I am in the shaman-space.
That's not easily "talkable" but has a lot to do with objects. As a shaman, I can craft an object for any purpose. If it accords with my own objectives.
Meaning. And I defined "meaning" as having the root of "means" and means being the path between now and next. "Meaning" is the same word as "purpose" .. and it is defined by the pressure of speciation.
SO .. to explain a little, I spent a half hour speaking to my son about an impending house move.
My wife decided the house we live in has become burdensome. The burden is the advancing gentrification radiating from the nearby city - we feel it as an oppressive cloud. So we look at a place far to the west that is totally isolated - as our lifestyles have become .. bar the internet - where we both make our livings.
We are totally un-integrated with our community, because all around us are renteures - slag-dumps - retirees from the city, from whom are being extracted their life-times stored wealth for the rest of their expected longevity.
Well... I retired from that 10 years ago and it's not the end.
So I have to educate my home-schooled son about snake awareness - how to keep the lower legs fortified from the 10 most deadly snakes in the world .. but not to worry - we just make a lot of noise, and they, being shy creatures will move away. And they don't actually live to bite humans.
SO we are locally divorced because everyone is divorced .. running through channels designed to extract their increase and they are all poverty-stricken no matter what their annual income is.
So we don't need a community - in this age, there is no community.
We will go away - beyond the definitions. And when it all explodes, we will gravitate a tribe from the refugees, and we will start it all over again. Or die .. which is the more llikely outcome.. but between now and then, we are going to have a s**tload of fun. On our own terms.
Jul 10 2013: Thank you for defining words that are used differently in Australia.
One issue many people raise about cities is that many people are disconnected or not bonded in any significant way with neighbors whose purpose in living there is different. One of the things Jane Jacob considered a strength of the city was exactly the choice of whether to be/feel separated or to connect with others and within connection, the extent of connection. I know people I see every day and talk with whose names I do not remember. I still value the sort of regular connection we have.
I don't recall the age of your son. Will he miss friends when you move farther out?
Jul 12 2013: Hi Fritzie,
My son is 12 .. he is autistic .. but a lot less autistic than when he was 6.
I didn't actually want to become this isolated .. I'm naturally gregarious .. then with the child being so radically different, all the common basis dissolved.
Before all that, I was in a top earning job as a corporate consultant, living in the epicentre of the city with everything within a 1 minute walk .. anything at all. I suppose I was one of the 0.01% .. certainly as defined by money.
The isolation began with the birth, and then my wife was confronted with the true isolation of western culture - the abandonment of motherhood .. so she started an online mother's group .. I watched closely as it became a political unit and the target for marketeers, journalists, politicians and perverts. She solved a large swath of human problems keeping that community running.
The combined burden of all that precipitated our total disengagement with the urban paradigm - we had the best it could offer, and in the end one wakes up in the middle of the night wondering what your life is for .. with a glaring answer of "not anything at all" which is intolerable. SO we left.
Went into a semi-rural place and concentrated on music and art - as opposite as we could possibly get. Then the child got the ASD diagnosis when we tried to enrol him in a school.
So we read all the peer reviewed papers on autism, appointed psyches and confronted the mainstream education system to get our kid integrated.
After a lot of effort and research, we found that it's not a case of integrating into .. we would have to change the entire world to correct the skew. And so we have done.
It's exhausting. My child is no longer easily defined as ASD. The education department has produced a handful of people who understand that it's all a sham, and we have a place in reality.
Better than waking up at 4am with no reason to be on the surface of the planet. Popularity becomes a craft .. at needs.Jul 12 2013: Here we go!
Courtesy of my wife Ayca:
see what I mean?
BTW - Creative Copyright (CC) is granted on these images - if you need to quote sources.Jul 12 2013: Are the images your son's work? I am so glad he is thriving.
Here is another piece you may find interesting about creating informal community: http://www.thepolisblog.org/2013/07/contested-urbanism-dharavi.html
Jul 12 2013: No The images are by my wife - it was very quickly done .. and hard to draw when larfing so much. (there's more production-grade stuff on the website .. but the cartoonic simplicity is sufficient for that particular message.)
Thanks for the link. David Harvey also promotes the work of Lefebvre .. personally, I think there is only one dynamic at work in urbanisation: money. That's the only reason to go into a city, and if you don't have an exit strategy you get trapped there.
But, whatever happens, humans form up into tribes. I'd like to look at the study done by the group in your link to get access to the detail .. it might help as a cross section with Jared Diamond's recent book .. which is next on my reading list. From these bodies of work, I might be able to do a global abstraction matrix to identify disparate tribal formations much as I did for mercantile corporations .. and I expect it will be similar .. but perhaps with some evolutionary taxonomies added. How all that relates to comfort trade-offs and the role of technology is not clear .. but there's something there. The good thing about the corporate model is that it clearly demarks resource allocations .. and that measures entropy rates.
What I find particularly attractive about the link is that these "informal" megalithic urban phenomena accumulate "by default" and are not helped at all by top-down interference. If you put aside the artificial motive of money for a moment, that leaves a skeletal structure that requires no planning or education .. a property of the species as it were. Even slime mould does this when it gets a resource.Jul 12 2013: You have probably heard the TED Talk about the informal economy and the amount of commerce therein. I don't remember now the speaker.
I very much enjoy art brut and mistook the images for that sort of work. There is an arts magazine that features it, called Raw Vision. That work ranges from the quite simple to the incredibly complex.
Mitch SMith
Jul 12 2013: Art is being profoundly changed by the internet right now.
- +1
It's a phenomenon that I refer to as "the ghost in the machine".
The tool shapes the work.by virtue of its limitations. For instance all the music called "techno" arises from what is easily done with synthesisers. If you have a lathe, things tend to be cylindrical.
In reciprocation, the tool also alters the brain - the tool map becomes part of the body definition and therefore enters the metaphorical/symbolic definition of the world view.
So we have these 3 main forces exerting on us that shape us:
1. the stasis of the proto-self (comfort),
2, the changing landscape of day-to-day environment (discomfort).
3. the limitations of the tools (potentiation)
So I suppose you could match motives to each of these forces .. so .. call them intrinsic and extrinsic motivators plus a topological definition of potential means.
I'll think on that.Jul 1 2013: To earn comfort we spend our lives in a 10 by 10 cubicles...perfect worker bees.
To earn comfort..
We Give up freedom
freedom to move.
freedom to remain close to the nature and enjoy it.
Jul 3 2013: The tortoise is adapted - it has a thick shell.
The human is adaptable - it's skin is as thin as paper.
In times of teeth and claws, the tortoise rules, the human takes the tortoise's shell.
In times of fast floods and running Lava, the turtle dies and the human is already running.
There is a universal balance between adaptation and adaptability.
We have swift adaptability .. how swift is enough?Jul 1 2013: I'll just throw in a tangent here:
How about we look at the relationship between comfort and "benefits".
For instance, most citizens in western "democracies" surrender their recourse to violence to the state in return for the benefit of having policemen available to protect them.
Then the policemen start becoming violent without due cause.
The benefit is eroded without the corresponding return of the right to commit violence.
Don't let us kid ourselves - violence is a primary tool of survival. Surrendering it is a major concession.
Jul 1 2013: America is an advanced society, relatively speaking. As an American I have the right to use force (violence) in protecting myself from a perceived threat. I would (violently even) oppose any government effort to seek my surrender of my right to defend myself with "equal force". Now, about trade-offs, I offer one example. I willingly trade my right to privacy for the right to access the Internet and its immense benefits. I know "they" can, and probably do, record every keystroke I make. I choose not to make the same trade-off to participate in the social media where the benefit is far less influential for me. Anyway, I think the short answer to your question is "privacy".
Jul 3 2013: I agree.
The object of my post was to demonstrate the manifold aspects of comfort.
Here is the great power of TED. The participants in these talks and commentaries are united by one single strength - honesty. Even if we cannot agree with the divergent stances, we can acknowledge the earnest expression of them.
That gives rise to a rare value - the ability to discern multiple aspects of a single subject. At best it can reveal dimensions of a problem that are not obvious, at worst it can reveal common misconceptions.
Either way - it allows us to become wiser than we can each be alone. I find that enormously encouraging - and I thank you for your contribution.Jul 1 2013: Are you thinking of things like using fuel and electricity to keep living spaces within a narrow band of optimal temperature rather than allowing greater variation of heat and cold? Perhaps using private automobiles for the sake of privacy, time-saving, and control of ones mobility in lieu of using public transportation? Using artificial lighting rather than waking and retiring with the sun?
Jul 1 2013: Yes,
As a rough measure, one might use the human body as a metric.
For instance:
Our bodies seem to require some level of motion to maintain robust health.
There is some evidence that we need electrical grounding for many cellular functions to operate properly - polymer-soled shoes are not good for that.
The lymphatic system relies on a certain level of deep breathing for circulation.
Over-washing the skin actually makes us more vulnerable to infection.
Then there are the radiation issues with cell phones and other electronic gadgets.
All these things seem to have polar extremes .. water is good to drink, but easy to drown in.
Should all these things be taken in isolation? Or is there a common dynamic that can be employed for forming adaptive policy?
Jul 1 2013: As you write, what is common is that more is not always better (of use of particular technologies or of anything else). There are ranges of "better" points (I am deliberately not using the word optimal, as I doubt there is typically a unique best level of use), which differ for different people.
Comment deleted
Barry Palmer
Jun 30 2013: It has occurred to me that perhaps historians will single out the Twentieth Century for teaching the world that the costs of some forms of technology can be too high.
- +1
I can remember the first time I saw a news story about some tree huggers, trying to save some trees from being cut down. I think I was in my early teens. We all thought this behavior was bizarre, possibly crazy. It took a few years before we heard the word environmentalist, and even then this seemed a strange concept that threatened progress. It caught on and became mainstream. Now we are all working towards sustainable everything, and zero environmental impact. Soon healthy lifestyle will be a major factor in technology and city design, architecture, career choice, etc. It seems to be very difficult to learn what should be obvious.Jun 30 2013: A very good question, and extremely difficult to answer comprehensively.
Anyone who makes broad use of modern electronics has traded off privacy.
Everyone who uses an alarm clock to get up in the morning has given up sleeping as long as we need. Inadequate sleep results in further costs from mental and health problems.
Automobiles have many costs, both direct and indirect. We have turned paradise into a parking lot.
Too many times I used the TV to keep our children occupied; now, considering the trade-off for that is scary.
If I tried, I could probably go on for hours. Its almost too frightful to consider, especially when you consider the societal costs plus the personal costs. How many extremely bad decisions have been made by managers and politicians because they did not get enough sleep?
Jul 1 2013: Yes, it's a difficult one.
I get a feeling that there might be some simple metric .. something to do with how humans balance long and short term decision making. There's a lot of material on this with offering long term Vs. short term rewards (would you like a dollar today or 2 dollars next year?).
I keep thinking of that escalator leading up to the exercise gym.
Barry Palmer
Jul 1 2013: It seems that you are more interested in the personal trade-offs, rather than societal trade-offs.
- +1
That escalator is indeed interesting. Apparently, the proprietor considered it to be a good investment. Entrepreneurs can be a good source of behavioral insights.
Jul 1 2013: What societal trade-offs did you have in mind?
It would be interesting to explore the synergy.
Yes - entrepreneurs are adapt at harvesting advantage - it is a deeply psychological art, well worth examining.Jul 1 2013: Anyone who wants to search this literature could use "discount rate" as a key word.
Jul 3 2013: Thanks for the suggestion Fritzie!
It's been a few years since I did a business case.
The good thing about applying monetized NPV is that it directly addresses advantage - if you accept that money is the unitisation of advantage.
From that basis one would have to look deeply at the profit motive.
Here is where it gets gnarly. For instance, I have noticed that successful entrepreneurs only apply discount rates as a bottom threshold for any particular venture .. they hire accountants to do that for them because their motive is not so much the profit, but the thrill of winning it - or the thrill of getting huge amounts of it. The accountants' mindset is not motivated in that way.
It occurs to me that the studies on the affects of dopamine are cogent here, but that all of it devolves, ultimately, to moral and ethical imponderables.
And externalities are often akin to common-sense - sometimes you can't know them until after they appear.
The main problem with comfort is that it manifests as a threshold - above which is survival, below which is sickness and death.
We then get a temporal frame .. for instance, it may be prudent to lay-in stores for winter if you want to still be alive next summer.
I think the core target of my question here is: how do we measure the upper threshold?
Diminished returns seems like a good place to start.Jul 3 2013: Are you saying that you think of sickness and death as below a low threshold, then comes survival but not comfort, and then an upper threshold defines the boundary between survival and "comfort?"
The concept of diminishing returns describes something continuous, whereas the concept of threshold sounds more discrete.
There is no reason in principle that you could not discount utility rather than money. It's about now versus later rather than about cash now versus cash later.
Jul 3 2013: Hmm I've not been clear ..
The lower threshold is like the bottom end of a range you could call comfort.
So Comfort begins at survival, goes through a range suitable for risk variability, but beyond that becomes discomfort via unintended consequences.
Adaptability infers a similar range .. a point at which the adaptability is exceeded by conditions.
I agree that money is not sufficient to fully measure advantage. There's something significant about that .. apart from utility - any ideas?Jul 3 2013: I see. You are looking for upper and lower bounds on comfort. And normally when people write diminishing returns, they mean diminishing marginal returns. I see now your different meaning.
Are you thinking, then, that utility is a concept connected to an individual and you want an aggregate measure?
Jul 4 2013: Well . yes.
But there's a dross in the forge .. so to speak . that is the fractal egress of the self.
This thing bothers me .. that I wanted to define a self as being a nucleus/membrane model ..and that they are essentially the same thing . and that the membrane is the thing that modulates entropy with the nucleus being the purpose of the membrane .. a definition .. or something like a standing wave. The membrane modulates entropy in negentropic and super-entropic flows (flux?)
.. in a way, these flows are independent .. I was looking for the singularity where the flows produced a vertical flux barrier that precipitates the explosive negation of the self ..
But what I am finding is that there is always a fractal escape path .. the self cannot be isolated .. no self can exist without relation to another - and the relation can connect through grain-definitions that exceed perceptional field-size.
There is an old traditional concept that describes the breaking of the circle .. so far it looks like there is no such thing as a circle in this universe - and that the break-point is not arbitrary.
So when I talk about diminishing returns .. I am looking at identifying the reality break-point of any particular confluence of negentropy.
I suspect that the exit point is the beginning of an exploration into the super-entropic field - each self creates the environment in which other selves must manifest in order to balance the conservation of entropy.
What I'm getting at is that the universal entropic bias is the entire genesis of the universe .. and that it need be no more than 1:1-(1/infinity). The interaction of the prime singularities. (Boolean = not no-thing .. not not = not is minus not not .. and has no negative)
Nearest to the break-in-the-circle are the diminished returns - nothing of this self can pass through without being a part of the self on the other side.
Look at us .. we do this miracle routinely.
Fritzie -
Jul 4 2013: There is indeed no question that our utility/satisfaction/well-being both affects and is affected by others. And in this we have the connection or interdependence among utilities. The social utility is not merely an aggregate of independent others.
- +1
Jul 6 2013: Yes - it is the true increase.
GDP assumes zero sum - which cannot be .. which is why it is always equal to population growth.
Reality does not care about population.
Humans are fools to think that GDP is anything but a lie.Jul 6 2013: I have never thought of GDP as attempting to capture quality of life. It is simply an indicator of stuff produced and its market value and tends, I believe, to be correlated with other things like availability of health care and sanitation.
Jul 7 2013: Mind wandered.. I've been looking at alternate interpretations of "wealth" .. specially to do with the source of negentropy .. and how/why it is stored.
Current economic practices assume that all increase is harvested and entropized by specialised individuals who's only function is to destroy increase.
(edit: That role might be available to evolutionary forces .. accelerated entropy does not seem the required pathway just now .. Sapolsky has some hints about an alternate path .. but it's not politic for me to point that out - no one wants to know just yet)Jul 7 2013: Could you rephrase the second paragraph?
Jul 8 2013: OK .. don't want everyone to get a heads up on that one just yet .. we'll keep it to ourselves ;)
But just between you and me and anyone else who can read .. do you think that subtracting humanity from the local entropic system is advisable?
The best of wisdom is that we should all go solar .. and that means we don't need anything else on the planet to remain alive .. the most complete monoculture in the universe!
What an achievement!
And so much for the quality of human wisdom :)
And don't worry - no one ever reads these long threads .. it is like the ultimate "cloaking" technology.


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Beginning in June, the Office of College Relations will be sponsoring a T-shirt design contest for Rosemont students. We'll give you a theme then you can let your creative juices flow! Several lucky students will have their designs printed on T-shirts that will be distributed around campus to students.
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|Pastor and
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John Owen, Baptism and the Baptists - our 2015 Annual Lecture
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