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DevilDriver is an American heavy metal band from Santa Barbara, California, formed in 2002. The band was originally named Deathride, however, due to copyright issues and the name being taken by several bands, Deathride changed its name to DevilDriver, which refers to bells Italian Wiccans used to drive evil forces away. The band's self-titled album was not well received by some music critics. Their second album, The Fury of Our Maker's Hand received a better reception with critics, and entered the Billboard 200 at number 117.
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CaspACE™ FITC-VAD-FMK In Situ Marker (Cat.# G7461, G7462), a fluorescent derivative of VAD-FMK, was used to label apoptotic cells in a population of leukemia cells for analysis by flow cytometry. This marker was able to discriminate between early and late Fas-driven apoptosis in Jurkat T cells. CaspACE™ FITC-VAD-FMK was thus found to be a reliable marker for labeling apoptotic cells prior to flow cytometry.
Francis Belloc, Olivier Garnier, Catherine Boyer and Francis Lacombe
Several pathways lead to apoptotic cell death through the cleavage of zymogens and the generation of specific proteases called caspases. The caspase family can be subdivided into three classes: initiation, amplification and executioner. These classes are generally defined as follows: i) initiation caspases activated by death receptors (i.e., caspase-8); ii) amplification caspases activated by initiation caspases or mitochondrial molecules (i.e., caspase-9); and iii) executioner caspases activated by upstream caspases (i.e., caspase-3). All caspases are cysteine proteases and cleave their substrates immediately after an aspartate residue.
Activation of executioner caspases serves as an early marker of apoptosis for flow cytometric studies (1)
. However, the fluorogenic substrates needed to analyze these enzymes are not cell permeable. Some antibodies are able to specifically label the cleaved form of caspase-3 and can be used in multivariate analysis to reveal early apoptosis in leukemic cells (2)
. This approach necessitates cell fixation and permeabilization, and it is time-consuming.
The inhibitor peptide, CaspACE™ VAD-FMK In Situ Marker, is bound with varying affinities by most caspases. Thus, a fluorescent, cell-permeable derivative of VAD-FMK is potentially an interesting tool for flow cytometry analysis of the global activation status in the caspase pathway.
CaspACE™ FITC-VAD-FMK In Situ Marker (Cat.# G7461, G7462) was added directly to the cell culture medium (HEPES-buffered RPMI 1640 with 10% fetal calf serum) at a final concentration of 5µM (1µl stock solution in 1ml of medium). After incubation for 20 minutes at 20°C, the cells were centrifuged, washed in 1ml of PBS and fixed in 1ml of 0.5% formaldehyde for 30 minutes at 20°C. The cells were centrifuged again and suspended in 0.5ml of PBS prior to flow cytometry analysis.
Flow cytometry analysis was performed using an EPICS® XL Cytometer (Beckman-Coulter). Fluorescence was measured at 530nm (excitation of 488nm). Fixation was found to facilitate the discrimination between fluorescent and negative populations. The negative population was defined as the higher population of the untreated sample. However, the experiment described in Figure 1 was performed without fixation, as the intent was to measure the mean fluorescence of the whole population and not to discriminate between positive and negative cells. The cell sorting experiment was performed using an Elite cell sorter (Beckman-Coulter).
The FITC-annexin V kit (Immunotech, Beckman-Coulter) was used to detect apoptosis as specified by the supplier. Briefly, Jurkat T cells were suspended in Ca2+-rich binding buffer, and 5µl of FITC-annexin V were added. After a 10-minute incubation at 4°C, the cells were analyzed using an EPIC XL cytometer. The fluorescence was measured as described above.
The cells were incubated in culture medium with either 1µg/ml acridine orange or 0.5µg/ml Hoechst 33342 (Molecular Probes). Cells with either condensed chromatin or fractionated nuclei were identified as apoptotic by fluorescence microscopy. A minimum of 100 cells were counted for each sample.
To be a good marker of apoptosis, the CaspACE™ FITC-VAD-FMK In Situ Marker must move easily in and out of healthy cells and remain anchored inside apoptotic cells. The experiment described in Figure 1 was performed to verify this property. FITC-VAD-FMK (5µM) was added to Jurkat cells, and the evolution of cell fluorescence was monitored every 4 minutes by flow cytometry. The uptake of the fluorescent marker by the cells was accomplished in less than 4 minutes, after which fluorescence remained stable for 30 minutes (Figure 1). When the cells were washed by centrifugation and suspended in marker-free PBS, a steep decrease in fluorescence was observed, suggesting that greater than 90% of the FITC-VAD-FMK had been washed free from control cells. However, when the cells were pretreated with a Fas-agonist antibody to induce 80% apoptosis, their fluorescence remained three-fold higher than that of the control (Figure 1). These results confirm that the CaspACE™ FITC-VAD-FMK In Situ Marker is cell-permeable and is better retained in apoptotic versus healthy cells.
Jurkat cells (3 x 105/ml) were incubated for 3
hours in the absence (control) or the presence of 0.1µg/ml of anti-Fas IgM (7C11,
Immunotech). After addition of 1µl/ml of FITC-VAD-FMK (5µM final concentration), the
cells were incubated at 20°C and fluorescence was measured by flow cytometry every 4
minutes. After 30 minutes, the cells were pelleted and suspended in 1ml of PBS.
Fluorescence measurements were resumed again every 4 minutes. The values represent the
mean fluorescence channel value as a function of time.
One of the advantages of the cell-by-cell analysis afforded by flow cytometry is the ability to perform population analysis and thus account for the heterogeneity of samples. Figure 2 shows that the response of Jurkat cells to the Fas-agonist occurred in two-steps. Following FITC-VAD-FMK addition, a small population with low fluorescence intensity was observed. This low fluorescence was also observed in the sample in which apoptosis was not induced (Figure 2, 0 hour). This population increased progressively during 2 hours of treatment with the Fas agonist antibody. After 2 hours of treatment, a population with high fluorescence intensity appeared, and this population increased during the subsequent hour.
Jurkat T cells were treated with 0.1µg/ml of anti-Fas IgM to induce
apoptosis. Aliquots were removed at 0, 1, 1.5, 2, 2.5 and 3 hours and incubated with
5µM CaspACE™ FITC-VAD-FMK In Situ Marker for 20 minutes. The cells were then fixed
with 0.5% formaldehyde and analyzed by flow cytometry. After 2 hours of treatment, three
populations with different fluorescence intensities (negative, low and high) can be
Following treatment with the Fas-agonist, the no-, low- and high-fluorescence populations were sorted by flow cytometry, stained with the DNA binding fluorochrome, Hoechst 33342, and observed by fluorescence microscopy (Figure 3). The FITC-VAD-FMK negative cells were clearly identified as intact cells (Panels A–C). The highly fluorescent cells were identified as late apoptotic cells with condensed chromatin and fragmented nuclei (Panels G–I). The low-fluorescence cell population was more heterogeneous and included intact cells (Panel D), early apoptotic cells (Panel E) and late apoptotic cells (Panel F).
Fluorescence microscopy results for negative (Panels A–C), low (Panels D–F) and high (Panels G–I) fluorescence populations. The cells were analyzed as described in Figure 2 and sorted by flow cytometry after 3 hours of treatment. The sorted cells were stained with 0.5µg/ml Hoechst 33342 and observed for blue fluorescence under UV excitation.
The time-course evolution of the percentage of low and high FITC-VAD-FMK labeled cells was monitored by flow cytometry (Figure 4, Panel A). The low-fluorescent cell rate increased first (Figure 4, Panel A) and then remained at a constant level while the high-fluorescence cells began to increase (Figure 4, Panel A). During this time, the total number of apoptotic cells increased continuously (Figure 4, Panel B). The results of these experiments suggest that low-FITC-VAD-FMK-labeled cells are early apoptotic while the highly fluorescent cells are late apoptotic.
Panel A. Time-course evolution of the percentage
of low- and high-fluorescent populations in anti-Fas treated Jurkat cells after
FITC-VAD-FMK labeling. Panel B. Time-course evolution of the percentage
of apoptotic cells as determined by flow cytometry after FITC-VAD-FMK-(F-VAD) or
FITC-annexin V-(An-V) staining or by fluorescence microscopy after acridine orange-(A.O.)
staining in anti-Fas-treated Jurkat cells.
The time-course analysis of the whole FITC-VAD-FMK-labeled cell population was also compared with the number of apoptotic cells as measured at the membrane level by flow cytometry or microscopically at the nucleus level (Figure 4, Panel B). A good relation was found between the percentage of positive cells and the rate of apoptosis with both methods, confirming that FITC-VAD-FMK labeled cells were apoptotic. Similar results were obtained during Fas-driven apoptosis in another cell line, U937 (Figure 5). After three hours of Fas agonist treatment, an irreversible caspase inhibitor was added in excess and incubated for 15 minutes before labeling the cells with either FITC-VAD-FMK or annexin V. The addition of the caspase inhibitor decreased the percentage of FITC-VAD-FMK-labeled cells (Figure 5, blue bar) without affecting the percentage of annexin V-labeled cells (green bar). This suggests that active caspases are necessary for apoptotic cells to be labeled with FITC-VAD-FMK.
U937 cells were treated with anti-Fas IgM, and samples were analyzed at different times following FITC-VAD-FMK-( F-VAD), FITC-annexin V-(An-V) or acridine orange-(A.O.) staining. After 3 hours, the effect of a 15-minute preincubation with 20µM Z-VAD-FMK (Z-VAD) on FITC-VAD-FMK- (blue bar) or FITC-annexin V- (green bar) labeling was determined.
Finally, the labeling of U937 cells by FITC-VAD-FMK was compared to the rate of annexin V labeling after treatment with different apoptosis inducers (Figure 6). All spontaneous- (C), Fas- (anti-Fas), C2-ceramide-(C2-Cer) and daunorubicine-induced (DNR) apoptosis showed similar levels of fluorescent cells with both labels after 6 hours. This confirms that FITC-VAD-FMK can be used as a marker of apoptosis with flow cytometry, whatever the apoptosis induction method.
U937 cells
were left untreated (C), treated for 6 hours with 0.1µg/ml anti-Fas IgM, treated for 6
hours with 50µM C2-ceramide (C2-C), or treated for 1 hour with 1µM daunorubicin, washed
and incubated for 5 hours in drug-free medium (DNR). The cells were then labeled with
either FITC-annexin V (green bars) or FITC-VAD-FMK (blue bars) and analyzed by flow
cytometry. The percentage of positive cells was plotted for each treatment.
As shown here, FITC-VAD-FMK can be used to label apoptotic leukemic cells for flow cytometry analysis. The percentage of apoptotic cells detected using this marker is in agreement with other methods. Interestingly, FITC-VAD-FMK labeling was able to discriminate between early and late apoptosis through fluorescence intensity (Figures 2–4). These intensity changes may be related to the activation of initiation (early) or executioner caspases (late). The FITC-VAD-FMK marker was able to detect apoptosis induced by several molecules, including ceramide, while no caspase-3 activation was detected in this pathway (3)
FITC-VAD-FMK should be a useful tool to reveal caspase-dependent apoptosis by multivariate flow cytometry analyses. A reliable negative control, however, will be necessary for analyzing samples of heterogeneous cell populations such as blood or bone marrow. Currently, nonapoptotic controls are not easily available for these samples types.
Finally, although FITC-VAD-FMK binding can be considered as a good marker of apoptosis, it is true that general caspase activation is not exclusive for apoptosis and can also occur in some inflammatory and differentiation processes (for review, see reference (4)
Scientific Style and Format, 7th edition, 2006
Belloc, F., Garnier, O., Boyer, C. and Lacombe, F.
CaspACE™ FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of Apoptotic Cells.
[Internet] 2000. [cited: year, month, date]. Available from:
American Medical Association, Manual of Style, 10th edition, 2007
Belloc, F., Garnier, O., Boyer, C. and Lacombe, F.
CaspACE™ FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of Apoptotic Cells.
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An age-old relaxation aid that creates an object to beautify your space and calm your mind, the tradition of artistically tended miniature trees was originally passed from China to Japan and has persisted since at least the 8th century. With its dark green foliage, this easy-to-grow Sego Palm is ideal for that office or home spot that doesn't receive much sunlight.
Sego Palm is an indoor bonsai. Plant height varies from 10" to 16". Plant arrives potted in a container. It takes several weeks for indoor bonsai to acclimate to a new environment. During this period, your bonsai may shed up to 20% of its foliage. This is normal.
For best results, place your indoor bonsai near windows facing southeast or west. Exposure to sunlight enables your bonsai to replace foliage it may have lost. If you do not have a place for your tree to receive adequate sunlight, the use of a grow light is recommended. Indoor bonsai will flourish if grown outdoors on a patio or deck during summer months. The ideal placement is where the bonsai receives morning sun and afternoon shade.
Cannot ship to Canada, Alaska, or Hawaii.
Founded by Brussel Martin, Brussel's Bonsai has been a destination for bonsai hobbyists for over 30 years. This Mississippi company now occupies 175,000 square feet of cutting-edge greenhouse space, turning boutique operation into America's largest importer and grower of fine bonsai, all of which come potted in elegantly coordinated planters.
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by Luca Penati
Category: Analyst Relations, B2B, Global, Tech PR, Technology
The Technology Practice at Ogilvy PR today announced the launch of ACE (Analyst Community Engagement), a new global service designed to give technology companies an informed, strategic and measurable approach to industry analyst relations.
Why launch an AR service in 2012?
Two main reasons:
We are launching ACE because Analyst Relations is the most global of all marketing functions, and we believe we have the global coverage and expertise needed to support any kind of company on their AR needs. With a global team based in New York, San Francisco, Chicago, London, Hong Kong, Beijing, Singapore and Sydney.
Ogilvy PR has a unique PoV that can really help AR and PR work better together and speak the same language, making AR a strong asset for marketing, sales and, why not, corporate communications as well. We believe that the analyst world consists of "Deal Makers" and "Perception Makers", and that the true value of AR is in knowing how to distinguish between the two, and tap the right ones to influence sales cycles as well as shift market attitudes and perception. In other words, in the first instance, AR support sales, while in second one, AR supports reputation, and therefore PR.
If you want more information you can read our press release and check out this video.
I'd love your comments on this and what you see at your firm, regardless of whether you are in-house or on agency side.


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All hail the mighty Cardinals - the dominant force of the 2012-13 season from start to finish, and National Champions for the first time since '86! This spectacular poster was published to commemorate the University of Louisville's 2013 tournament championship, as Rick Pitino's team capped a well-deserved run to victory with a win over the upstart Wichita State Shockers in the semis, and Michigan in the final. For fans of inspirational guard Kevin Ware, the dynamic Peyton Siva, monster big-man Gorgui Dieng, Chane Behanan, Tim Henderson, Wayne Blackshear, Luke Hancock, and the rest of the squad, this one's for you! With an amazing design that previews the raising of the 2013 Championship banner into the rafters at the KFC Yum Center, this one will have you swelling with red pride with every glance. Championship posters are always published in limited quantity for a limited time - order now so you don't miss out!
Product Specifications:


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Track your day on the mountain
Overview of Mountain High
Never miss a powder day
for Android & iPhone
Copyright © 2012 Mountain High. All rights reserved.
Mountain High Resort is located in the Angeles National Forest under special use permit from the Forest Service. Facilities are operated to prohibit discrimination on the basis of race, color, national origin, age, religion, sex, disability, familial status or political affiliation.


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Twin 10GBASE-T Ethernet maximizes connectivity, minimizes expense while 5th generation Intel® Core i7 processor technology delivers higher performance and greater functionality than previous generations of SBC
The GRA113D enables high-performance computing and visualization applications in a SWaP-constrained, rugged environment
The QVXI-1553X provides new levels of performance and flexibility for MIL-STD-1553A and B Notice II on a C-size card.
The Mark VIe Distributed Control System is a flexible platform for multiple applications. It simplifies operations and reduces risk and costs while providing excellent performance and reliability.
Provides a rugged yet highly flexible COTS computing platform suited for a wide variety of avionic, vetronic and navtronic applications requiring rock-solid reliability.


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Home > Electronics > Accessories for Apple > iPad Speakers > Contour 200i Air Wireless Music System with AirPlay and Dock
Contour 200i Air Wireless Music System with AirPlay and Dock
Bring Airplay technology into your music world and wirelessly stream music.
Contour 200i Air is a compact but powerful digital music system featuring wireless streaming technology from Apple®, AirPlay, and a dock for your iPod®, iPhone®, or iPad®.
AirPlay lets you stream music wirelessly to Contour 200i Air from a compatible iPod®, iPhone®, or iPad® or from iTunes® on your computer using a Wi-Fi or Ethernet connection.
AirPlay enables both the streaming of high quality audio plus two-way synchronization – change volume on one device and the level is mirrored on the other. And if you want to set up a multiroom wireless speaker system, simply put a Contour 200i Air in any room and you can send audio to them all simultaneously from iTunes. Bring thousands of radio stations, and more to your Contour 200i Air with the Pure Lounge app. Available free of charge and suitable for the iPod touch®, iPhone®, and iPad®, the Lounge app gives you free access to thousands of Internet radio stations, on demand programs, podcasts, and more.
To make sure you get the most from your music Contour 200i Air has been engineered to deliver 36W RMS of highly detailed audio with twin bass ports helping to provide a rich rounded sound. The built-in dock supports most iPod®, iPhone®, and iPad® models and enables you to charge your device and control playback using the supplied remote control.
Features Include:
- Easy Network Connection Setup
- An Input for Connecting Other Audio Devices
- A Digital Output for connecting to an External Amplifier or Hi-Fi System.
Dimensions: 6.90" L x 14.60" W x 9.00" H
Contour 200i Air Wireless Music System with AirPlay and Dock
Contour 200i Air Wireless Music System with AirPlay and Dock
|Apple compatibility
|Supports most iPod, iPhone and iPad models
|2 full-range 3.5" drive units with twin downward firing bass ports
|Audio output
|2 x 20W RMS
|Input connectors
|12V DC power adapter socket. iPod/iPhone/iPad connector. 3.5mm Aux-in for auxiliary devices. Ethernet socket
|Output connectors
|Digital coaxial socket for digital audio
|Remote control
|Infrared remote control. CR2025 button cell battery included
|Mains power supply
|100-240V, 50/60Hz 12V DC 2.0A external power adapter
|CE marked. Compliant with the EMC and Low Voltage Directives (2004/108/EC and 2006/95/EC). ETSI EN 300 401 compliant
Contour 200i Air Wireless Music System with AirPlay and Dock
Our innovative products will provide you and your family with years of enjoyment. And with the Brookstone Protection Plus service and replacement plans, you'll have the added peace of mind knowing that if the unexpected does happen, we will simply repair or replace your covered product. Our enhanced coverage goes above and beyond the manufacturer's warranty, saving you time and money.
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"J-Term" is a month-long semester where unique opportunities abound. Test drive a career with an internship, take a travel/study course, or sign up for an engaging seminar.
internships galore
Our students intern everywhere. From law offices to Capitol Hill. From a local television station to MSNBC. From a doctor's office to the Centers for Disease Control and Prevention.Internships with Hollins
see the world
Students often travel to a variety of countries, including Ireland, Greece, Turkey, and the Caribbean.
short term seminars
First-year students choose from an array of seminars. Examples of recent courses:
- "The Global Game: Futbol, Politics, and Culture"
- "Smart Women: The Female Scientist in Film"
- "Think It, Ink It, Bind It"
- "Photography: The Scanner as Camera"


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Pool Ball Cleaning Tools
As the centerpiece of any pool table, pool balls are usually the first thing that people notice when entering a gameroom. These cleaners will help you keep your balls looking great and will ultimately extend the life of your ball set as well as your table cloth.
Chem Pak Billiards and Pool Ball Cleaner and Polish
Our Price: $6.36
Save 20%Chem Pack Ball Cleaner is specially formulated to clean, polish and protect in one easy application. Easy to use by hand or machine. Just shake, apply directly to the ball, rub well, set to dry and buff to high luster. Return your ball sets to their former beauty and glory with Chem Pak. 8 floz. Learn More


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Description:Emulex Dual Port Virtual Fabric Adapter II Network AdapterPart Number(s)Manufacturer Part# 95Y3751
Overview:The Emulex Virtual Fabric Adapter II for IBM System x is a high-performance, dual-port network adapter for 10Gb Ethernet (10GbE) networks. It offers the benefits and flexibility of I/O convergence with a single end-to-end solution. Stateless TCP/IP offloads and TCP chimney offload provide maximum bandwidth with minimum use of CPU resources.
CategoryNetwork Adapter
Sub-CategoryEthernet Adapter
Part Number95Y3751
Products ID461371
Connectivity TechnologyWired
Data Link Protocol10 Gigabit Ethernet , Fibre Channel over
Interface TypePCI Express 2.0 x8
Data Transfer Rate10 Gbps
Form FactorPlug-in card
[ Manufacturers Specs ]


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University Club of Montreal4 Reviews
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Celebrating memorable weddings for 130 years!
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'Planet of the Apes: Cataclysm' Covers by Noto, Hardman and Quinones [Art]
The latest from BOOM! Studios' well regarded Planet of the Apes comic book franchise, Cataclysm is the publisher's first ongoing series set in the world where apes rule and mankind is hunted and enslaved. Set just a few years before the events of the original 1968 film, Cataclysm is writt


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There is going to be a 6X6X3/8 HSS post ""That I already fabricated"" on the corner. It has to clear the guard when they swing the tree around to cut it to length.
In the corner, those are a little globy tacks, the plate was cherry hot and the setting I was on was hot for complete penetration. I'll grind those up and put a nice uphand T-91 fluxcore bead in there
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Processor mod. Catwalk extension


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GUESS Originals Collection A modern take on the sexy denim revolution we started in 1981, this jumpsuit is an icon in itself. The mid-weight stretch denim is washed to a crisp white shade, making it essential for the warmer seasons. A strapless, corset-style bodice and skinny fit hug your shape to give you a forward-looking fashion statement that's inspired by our past.
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I am a female who does a lot of D.I.Y. Presently use a fairly old Black and Decker Drill. Would like to buy myself a new cordless drill which will drill holes, and screw, screws straight into walls, wood and concrete etc.
Would appreciate any advice on which type of Cordless Drill is the best and easiest to use.
Many thanks


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poppy high chair
From high chair to 'my chair'
Stylish, safe & easy to clean - all the essentials covered!
"Super easy to clean and works very well for my chunky 1 year old. Curved tray keeps his food from going overboard!"
Sarah – Mother of Bennett
The poppy ™ high chair is HOT! because:
- value for money! poppy high chair converts to a child seat for ultimate longevity of use
- soft & spongy aerocore seat, for maximum comfort
- aerocore is insulating, hypo-allergenic, UV resistant and non-toxic, as well as being waterproof & wipe clean!
- light weight to move & easy to store away - only 4kgs / 8.8lbs
- wipe clean & dishwasher safe food tray
- food tray clicks in & out with a one button press action
- passive harness to keep your baby safe in their seat until the food tray is attached
- 5 point safety harness
- high chair suitable from 6 months to 3 years, or maximum load of 20kg / 44lbs
- 'my chair' suitable from 6 months to 5 years, or maximum load of 20kg / 44lbs


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Refugio County Chief Appraiser Connie Raymond said the appraisal district "is in dire need of more space. And we have been looking for quite some time."
She said a building or relocating were considered.
But then the adjoining office was offered to the district at a purchase price of $25,000, and an estimate for renovations was made at $150,000.
"All we need is your approval," she told the council.
And she said the Appraisal District has the funds for the purchase and renovation. So no city funds would be needed.
"All I need is approval to move forward," she said.
The council voted unanimously to approve.
Raymond said the city is the fifth entity to give approval.
"We need approval from all the cities, school districts and county," she said.
The council also heard from Donald Goldman of the auditing firm of Goldman, Hunt & Notz in Victoria.
Goldman said the city's 2011-2012 audit was done by U.S. auditing standards.
He said the city received a "clean opinion," the highest opinion given in an audit.
He said because of good financial stewardship, the city could operate for six months if it had to with no income.
The cities activities, he said, cost $3.6 million.
"This is big business," he said.
He also mentioned that the city had $5.2 million in assets, an increase of about $100,000.
And he praised the city for not amending its budget over the year like so many other cities do.
Also, the council approved a resale-of-properties list of seven properties presented by Alice Garcia of Linebarger Goggan, attorneys at law in Corpus Christi.
The properties did not sell at the county level, so a list was provided that people bid on the properties.
"Approve this, and the properties will go back on the tax roll, and you can start collecting taxes," Garcia said.
Properties included the following: 505 E. King St., 509 E. King St., 301 Shelly St., 207 Barefield St., 1108 Bayou St., 209 W. Federacion St. and 205 E. South Street.
The council also divvied out motel tax money to various 4-H organizations and to the Texas Independence Wine Classic.
Here's the amounts each received:
4-H Livestock Judging Team, $3,179; 4-H Booster Association, $3,567.45; and Texas Independence Wine Classic, $12,155.
The Refugio Chamber of Commerce amount was tabled for a meeting at a later date.
The council also approved a Racial Profiling Report from Police Chief Andy Lopez.
Lopez said by law, the department is required to provide the report.
He said the report shows how many people were stopped by ethnicity, gender, for searches, number of consents, arrests and warnings.
He said 6,656 tickets and warnings were given last year.
"That's a lot for a six-officer department," he said.
The council also approved 25-cent raises for two employees finishing their probationary periods: Jarren Bland in the water department and Elston Howard Jones in the wastewater department.


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Am I an ******* for laughing about this?
The latest YOLO fatality is rapper Ervin McKinness, who performed under the names Inkyy and Jew'elz. His last tweet? "Drunk af going 120 drifting corners #F**kIt YOLO." Not long after he tweeted it, the rapper, along with three other passengers and the driver, were dead, after their car sped through a red light and crashed into a wall in Ontario, Calif.


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Chipset Diagram
Product information
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|Features and benefits
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Additional information: 1 2 3
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1. Requires an Intel® Hyper-Threading Technology (Intel® HT Technology)-enabled system, check with your PC manufacturer. Performance will vary depending on the specific hardware and software used. Not available on the Intel® Core™ i5-750. For more information, including details on which processors support HT technology, visit www.intel.com/content/www/us/en/architecture-and-technology/hyper-threading/hyper-threading-technology.html.
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text: Sorry for making a whole thread on this guys, but i'll be in Boston Sunday for a WWE event, and need to find a bar to watch us whoop the Raiders. Does anyone know if there's a Dolphin bar in Boston... I know the odds are slim considering the location, but it cant hurt to ask. Thanks and LETS'GOOOO!!!!!!!!


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qHTS Assay for Inhibitors of Fructose-1,6-bisphosphate Aldolase from Giardia Lamblia: Rabbit FBPA Selectivity
The objective of this project is to obtain compounds that inhibit Giardia lamblia growth. G. lamblia is a human pathogen which afflicts impoverished nations; it is the most common cause of outbreaks of diarrhea in the United States. During the course of our study of potential drug targets in Giardia, we have identified the Class II (Zn2+ functions in electrophilic catalysis) more ..
BioActive Compounds: 59
Related Experiments
NIH Chemical Genomics Center [NCGC]
NIH Molecular Libraries Probe Production centers Network [MLPCN]
MLPCN Grant: MH085699-01
Assay Provider: Osnat Herzberg, University of Maryland
NCGC Assay Overview:
The objective of this project is to obtain compounds that inhibit Giardia lamblia growth. G. lamblia is a human pathogen which afflicts impoverished nations; it is the most common cause of outbreaks of diarrhea in the United States. During the course of our study of potential drug targets in Giardia, we have identified the Class II (Zn2+ functions in electrophilic catalysis) fructose-1,6-bisphosphate aldolase (FBPA) as an excellent candidate for drug targeting. FBPA is a key glycolytic pathway enzyme, essential for G. lamblia survival. The human FBPA belongs to the Class I aldolases, which have a different substrate binding site structure and a radically different catalytic mechanism (employing a lysine-Schiff base intermediate). Therefore, affinity-based or mechanism-based inhibitors of the Giardia FBPA are expected not to interfere with the catalytic function of the human FBPA.
A glyceraldehyde-3-phosphate dehydrogenase/triose phosphate isomerase/NAD/arsenate coupled assay was adapted to the 1536-well HTP format for use in the primary screen. This was done by coupling it to a diaphorase/resazurin/resorufin reaction that can be monitored by fluorescence (excitation, 544 nm; emission, 590 nm). Compounds identified in this screen will be further evaluated by coupling the glycerol-3-phosphate/triose phosphate isomerase/NADH assay to a phenazine methosulfate/tetranitroblue tetrazolium color reaction. False positives due to the dehydrogenases and diaphorase inhibition will be eliminated, and selectivity assay will triage inhibitors of mammalian triose phosphate isomerase and the Class I FBPA. G. lamblia FBPA inhibitors identified by the in vitro analyses will be examined for growth inhibition of Giardia trophozoites, and for cytotoxicity in human cells.
This assay addresses whether hits in the primary screen are selective for Class I FBPA, by using rabbit FBPA enzyme in the protocol.
NCGC Assay Protocol:
1,Coupling enzyme reagent,2 uL,glyceraldehyde-3-phosphate
2,Compound,23 nL,3.68 nM-57.5 uM final concentration
3,Time,10 min,RT Incubation
4,Substrate/detection Reagent,2 uL,Substrate + enzyme coupling system + AmpLite Red kit
5,Time,20 min,RT Incubation
6,Detector,525 nm/598 nm (Fluorescence),ViewLux Fluorescence Read (Read 1)
1,Medium-binding black solid-bottom Kalypsys plates. FRD or Kalypsys dispenser.
2,Compound Library (10 mM ,1:5 dilution). Pin-transferred for a [final] range of 3.68 nM-57.5 uM.
3,RT incubation
4,FRD or Kalypsys dispenser, 2 uL of reaction mixture. Bottle kept at 4C and covered in foil to protect from light.
5,RT incubation
6,ViewLux Protocol 1194; Excitation filter wheel A (525/20 Pol; BODIPY TMR FP), Emission filter wheel B (598/25 S; BODIPY TMR FP S).
Keywords: Fructose-bisphosphate aldolase, FBPA , Giardia lamblia , Giardia, HTS, Inhibitors
Compound Ranking:
1. Compounds are first classified as having full titration curves, partial modulation, partial curve (weaker actives), single point activity (at highest concentration only), or inactive. See data field "Curve Description". For this assay, apparent inhibitors are ranked higher than compounds that showed apparent activation.
2. For all inactive compounds, PUBCHEM_ACTIVITY_SCORE is 0. For all active compounds, a score range was given for each curve class type given above. Active compounds have PUBCHEM_ACTIVITY_SCORE between 40 and 100. Inconclusive compounds have PUBCHEM_ACTIVITY_SCORE between 1 and 39. Fit_LogAC50 was used for determining relative score and was scaled to each curve class' score range.
Categorized Comment - additional comments and annotations
From ChEMBL:
Assay Type: Functional
Result Definitions
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: MH085699-01
Data Table (Concise)


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Past Debates: Wendy Kaminer
Chat with Wendy Kaminer, author of Sleeping with Extraterrestrials
13 January 2001
OK Wendy. This audience probably agrees with many of your points in the book, but you will excuse us if we will focus on whatever disagreements we might have. We are all committed to rational discourse and scientific evidence, but even within these parameters there may be ample room for disagreement, Which of course makes discussions like this one interesting to begin with.
That's okay. I usually find disagreement much more interesting than agreement.
OK, I'll start to warm things up a bit. You seem to downplay the biological evidence about differences between male and female humans, especially when it comes down to behavior. While I agree with you that any such difference doesn't imply anything about "better" or "worse," I thought your dismissal of neurobiological research on sex differences was a bit too fast. Since you accused Stossel of pseudo-science (perhaps rightly, I didn't see the show), I should point out that one of your arguments is not very well informed by biology either. In paraphrasing feminist Helen Hamilton Gardner you say that "no man's brain outweighed the brain of a whale. If intelligence were determined by brain weight, almost any elephant is perhaps an entire medical faculty." Except that it is well known that brain weight is correlated to body weight, and that any meaningful comparison can only be made only once this correlation is statistically accounted for. Any comment?
I don't pretend to be an authority on the brain, but neither were 19th and early 20th scientists. My point was that the scientists who associated brain weight with intelligence were acting on the basis of assumptions, preconceptions about male and female intelligence - not scientific inquiry.
That may be true (we don't really know that much about their personal ideologies). But why does that discredit modern research on gender differences in the brain?
As I recall (and I haven't reread the chapter on junk science recently, I was attacking the media presentations of research on gender and the brain and pointing out the ways in which prevailing social prejudice affects the framing of scientific research. My understanding of research into the human brain (which, i admit is pretty elementary, is that relatively little is known about gender differences in humans and even less can be said about their actual cognitive effects.
And I agree that scientific research can be biased by social trends, and it certainly has been in the past. But shouldn't a skeptic concentrate on the evidence rather than why it is being proposed? Isn't an attack on motives fall into the "genetic fallacy" (attack the group of individuals rather than their arguments)? But I agree with you that the current state of research is still too open to speculation one way or the other.
In any case, I didn't mean to discredit research into gender and the brain, I did want to show how our own assumptions about it shape the way its viewed. But, I thought I was adopting a skeptical approach to reports about brain research, by pointing out their biases. The problem with research on sex differences generally is that people don't view it objectively.
Wendy, in general I agree with the theme of your book ... the world has become much too irrational. But one bothersome point - use a placebo if it helps?!
Why not use a placebo if it helps?
So, Phil, why not put the placebo effect to good use? It *IS* an effect, after all.
Let me pick back up on placebos. Would religion fall into this same category? Is it therefore a good thing?
As for placebos - Yes, I think that for many people, religion is a placebo, and I hope I made clear in the book that I believe religion to be a "good thing" for many people as well. I don't consider myself particularly hostile to religion (or religions) per se; I am highly critical of the assumption that religious belief is an unmitigated good.
Wouldn't it be nice if people could be healed by rationally-based cures - that is, mentally feel JUST as good about those type treatments.
I think that it is falling onto a trap to consider the religious trappings attached to basic humanistic values as a necessary part of the placebo? I meant the supernatural trappings.
But many people are healed by "rational" cure, or medical science - and to the extent that state of mind affects health (consider stress and high blood pressure), placebos aren't necessarily irrational.
Well, a couple of weeks ago I found myself praying. I said, "Lord, just get me through this week---and maybe I'll believe in you!" If there's a god, it'd better have a good sense of humor.
But wouldn't it be good if people didn't NEED such placebos to be "healed?"
Perhaps I'm going a bit far afield, but I think this is related to the religion/placebo discussion. In the movie "The Matrix" the protagonist is given a choice between a blue and a red pill. The first one will allow him to continue living in an imaginary but relatively painless world. The second one will plunge him into a harsh reality. He chooses the second one, and so would most people if the question is put that way. Yet, we are talking about religion or some other forms of fuzzy thinking as good. Are we patronizing?
Believe it or not, my little "prayer" did help, by making me laugh at myself.
Actually, I think it would be bad if people who can now be helped by placebos (and were not helped by conventional approaches) came to find placebos useless as well.
But if "cures" are not based upon rational underpinnings, these people WILL come to find they are not helped.
Of course religious people would find our discussion of religion extremely patronizing and offensive, and i sympathize with their perspective. What's interesting about belief in god is that it has nothing to do with intelligence or the capacity for rational thought; to people who believe, the presence of God is as real as the chair that I'm sitting on.
Does that make God real?
Carl, it's probably the "trappings" that provide the placebo effect more than the humanistic values. Smelling the smoky air, listening to the organ and the choir, looking at the soft light coming through the stained glass--that's what lowers the blood pressure and makes you ready to go back to work on Monday.
Is God real? That depends on who you ask. God isn't real to me, but He/She/Whomever is quite real to millions of others.
I wasn't referring to the ritual but the notion of divine intervention and associated effects as trappings.
I always avoid, when I can, getting embroiled in discussion about the existence of God - it's not a question that's amenable to argument.
God is real to many, just a love is or hate is, but is also just like them in that it is constructed by a mind/body interaction. Which does not guarantee actual physical existence.
Self destructive behavior is not cured by things like religion that involve the placebo effect. It may not even be postponed long.
Hope you folks don't mind if I try to change subject a bit. I'd like to discuss your chapter on cyberspacy (after all, we are in cyberspace right now... ;-) We have read earlier for this book club Neil Postman and are familiar with his generally pessimistic attitude toward new technologies. I even agree with him to a larger extent than other members of this group. However, I cannot avoid the feeling that perhaps people were lamenting the end of civilization immediately after the invention of the printing press too. In fact, Plato has been known to say that writing is a curse because it leads people to stop thinking and rely on memorization instead. Apart from the obvious question of why then did he write so much (or why are you participating in an internet chat ;-), quite obviously civilization survived and indeed prospered as a result of both the invention of writing and of the printing press. Might not the same be true, with proper and perhaps even painful adjustments, of the internet?
Massimo, I agree with you about the Internet, and in writing about what will be lost in cyberculture, I didn't mean to imply that nothing would be gained. But it's much easier to view the past and predict what will be lost than to look into the future. I imagine that cyberspace will help create new ways of being intelligent and creative that will be quite foreign to me.
Yes, for example you correctly point out that perhaps the major problem with the internet is that one has little ideas about the reliability of different sources. Believe me, I experience this regularly with students presenting an article in Nature and a web link as if they were comparable sources. Yet, I already see plenty of signs that things will evolve pretty much like they did for the printing press and other media: some outlet will emerge as more reliable than others, and eventually the difference between the New York Times and the Washington Times will be as clear in cyberspace as it is in print. Or not?
Both newspapers are probably liberal, right? The internet will wrest control from the bastions of the liberal left :-)
Washington Times is the moonie paper.
Perhaps the excesses of some internet sites will even promote skepticism. It doesn't take long for bullshit to be called on usenet, where there interactivity.
Maybe, I wonder if the cyberculture (and the legacy of post-modernism, in general) will lead to the devaluing of reliability. In cyberspace, with so many voices, will everything be a matter of perspective?
Don't get me started on postmodernism; every time I hear the word I reach for my gun... (Unfortunately, I am a non violent person, so I don't have a gun).
The internet does allow for specialization and depth. TV doesn't, usually.
There were many shining moments for me in reading your book. One of those was in your criticism of TV and music being played in all public spaces. It is so difficult to find a quiet place to read and think in public, for all the noise. I am beginning to complain in restaurants and I'm letting them know that I choose to eat lunch where no music is played.
Re the increasing cacophony of public life - I do have an old-fashioned view that good, original thinking requires focus, which I think most people are incapable of attaining when in the midst of so much noise. I'm suspicious of multi-tasking.
In the chapter "The therapeutic assault" you draw an intriguing parallel, on which however I harbor some doubts. You compare conspiracy theories about aliens allegedly in the hands of the government with the recovery movement. You say: "denials of deeply felt beliefs - about spaceship crashes or child abuse - are apt to be considered confirmations of their truth." Perhaps there is something in common among all cases of human denial (and in fact recent neurobiological research seems to confirm this), but then stories about aliens don't have any more of a special relationship with the recovery movement than with anybody holding tightly onto any opinion in the face of facts. Is your parallel therefore too broad or too narrow?
Actually, stories about aliens have a very important relationship with the recovery movement: The alien abduction stories invariable involve allegedly recovered memories of being raped or otherwise abused by aliens. Alien abduction narratives, which reflected the cultural preoccupation with child abuse, were shaped by the recovery movement.
Good point about aliens and sex.
Hmm, but I thought alien abduction stories pre-date the recovery movement by decades. No?
Re tales of aliens - there are reports of extra-terrestrial sighting dating back to the 19th century, but I don't think tales of sexual abuse by aliens were common until the post-recovery years.
Wendy, you may be right on the sex-alien vs. recovery correlation. It would be interesting to conduct an in-depth sociological study on the matter. Skeptic magazine or Skeptical Inquirer should be interested.
I'd bet it's already been done. Let's do a literature search!
I think we need to be worried less about what is "real" to miscellaneous groups of people and more about what is "real" as supported by evidence.
But the study of how something unreal as to evidence becomes real as to belief is an important thing about human nature to know about.
Re the question about what is real - I agree that we need to focus on what we can know empirically, when we're talking about assertions that are amenable to empirical proof. I don't think it's productive, or even appropriate to put matters of faith (like a belief in the divinity of Jesus), to the test of empirical reasoning.
I agree with Melissa. I think skeptics often pay too much attention to the truth out there and not enough to how humans perceive it or distort it.
Why not treat the "divinity of Jesus" as we would the "story of the tooth fairy?" - is it because one is for an adult and one is for a child?
There's nothing to be gained by comparing Jesus to the tooth fairy. You will never dissuade believers that God is a figment of their imagination. Why not allow people their faith (you can't deprive them of it anyway) but try to encourage people to distinguish between matter of faith and science?
Sounds like the Martin Gardner approach.
I have no problem with people believing in Jesus or the tooth fairy. It's when they want me to behave according to the precepts of their imaginary friends that I get nervous...
I think that a majority of Americans would agree that religious people shouldn't be able to impose their views on others, but they disagree about the dangers of introducing religion into the public square, with public support.
Someone convinced ME that Jesus was a figment of my imagination. I was once a very strong believer.
People do lose their faith, but I don't think they lose it because of a rational argument, anymore than they gain it rationally. I suspect that people are temperamentally inclined to believe or disbelieve.
We have that very sort of disagreement going on within our group.
You may never change a true believer's position but I don't think that most people are really true believers, they just haven't been questioning the generally accepted.
The Sectarian Public Square chapter was a very strong argument against religious people dictating behavior to the rest of us.
Yes, about that chapter ("The sectarian in the public square"): you make an interesting argument that a pluralistic society in which secularism is more or less accepted depends on the continuous strife among religious sects. Ergo, a potential big enemy of freethought would be true ecumenism, which could lead all religions to gang up against atheists and agnostics. I find this scenario intriguing, albeit unlikely to actually occur in practice because of irreconcilable differences among religious peoples of different creeds. Do you see this as a real threat to modern American society?
No, I don't see ecumenism taking hold to a great degree. I think people are naturally tribal and sectarian and that part of religion's appeal is its exclusivity.
I think that other of religion's appeals are authority and certainty.
Don't overestimate the certainty of believers. They have their doubts and anxieties.
It is ironic that religious "certainty" is actually the least certain of all truths, isn't it?
Ironic - but as Wendy points out, certainty is quite possible is something is not susceptible to inquiry or rational thinking.
I think that for many people, religion provides hope as much as belief, or rather hope as much as certainty.
I was telling Melissa today that I found a quote from Arafat (of all people) about religious wars: he said that it is a matter of deciding whose imaginary friend is
What a wonderful quote, especially considering the source. Where did you find it?
I'll have to check the source, since it was on an Internet site... ;-)
I agree with that (about religion offering hope). The world can sometimes seem short on hope.
Hope can come from other sources than supernaturalism.
That's one of the first things we discussed. Wouldn't it be nice if people could find "hope" in just "being"
Religion is at its most dangerous when it offers certainty and most helpful when it offers hope.
I have one final question. When you ask "what fueled hysteria about recovered memories, child abuse, and conspiracies of satanic ritual abusers?" I find your explanation (in terms of resistance to women's liberation and a paradoxical help from the feminist movement) to be a "just-so" story, albeit an interesting one. Similarly, you say "like spiritualism, multiple personality disorder may have been a powerful if self-destructive metaphor for women struggling to forge alternative identities." Sounds good, but how are we going to test this kind of explanations, or - which is the same - why should we think that they are better than any number of other scenarios that one might come up with upon a moment's reflection?
I don't think we can always test explanations of social trends, and I was offering opinion, speculation; I didn't pretend to be offering facts. Speculation is interesting, and people can take it for what its worth. Social criticism, after all, is not a science and doens't, or shouldn't, pretend to be.
True. That is, however, what bothers me most about social sciences. There are exceptions, for example the research by historian Frank Sulloway on family dynamics and the effect of being a first vs. a later born.
Massimo, your discomfort with social science (which is more grounded in empiricism than social criticism) reflects the yearning for certainty, I think.
Not certainty, just a very likely maybe...
Well, gang, I think it is time to thank our guest for a great hour of free-ranging discussion, and for having written a book that stimulated several of us (more
than participated to today's discussion) to think.
People are complex systems and capable of believing *anything*. That's why your book, Wendy, is good - for asking us to reflect on what we are believing.
I find your comments and writings very helpful, enjoyable and encouraging, thanks.
My pleasure. Sorry if I couldn't always quite keep up.
Reading it was great fun. Thanks for writing!
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The Alpin Hotel is small but perfectly formed, it offers luxury in the centre of Borovets and is just 20m from the pistes. The atmosphere is peaceful and cosy letting you relax and recharge. If you want to party the nightlife is nearby; at the Alpin you get the best of both worlds; tranquility within a stone's throw of the resort's legendary entertainment.
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Globetrotter eats court after failed dunk
A Harlem Globetrotter almost trotted his way into the next life after an attempted dunk went awry in Honduras. The New York Daily News reports William "Bull" Bullard was trying to do an alley-oop dunk at the Nacional de Ingenieros Coliseum in Tegucigalpa when the hoop toppled over onto him. Bullard suffered a cut over […] [Read More]


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Few awards shows pack in the musical performances quite like the Americana Honors & Awards show, which returns to Nashville on September 18 as part of the Americana Music Festival and Conference.
More than a dozen acts are set to perform at the Ryman Auditorium that evening, including Rock and Roll Hall of Famers Stephen Stills and Richard Furay, Dr. John, Emmylou Harris & Rodney Crowell, Old Crow Medicine Show, Richard Thompson, John Fullbright, Shovels & Rope, Milk Carton Kids, JD McPherson, Mike Bub, Kelly Willis & Bruce Robison, Holly Williams and Duane Eddy.
Other big names will take the Ryman stage to present awards, including "Office"/"Hangover" star and bluegrass musician Ed Helms, filmmaker Ken Burns and Rosanne Cash.
Tickets to the Americana Honors & Awards show are $65-$85 and available at Ticketmaster.
The Americana Music Festival & Conference takes place September 18-22 and is open to music industry professionals and the general public.
Admission is $350 for members of the Americana Music Association and $450 for non-members. Admission includes access to sanctioned daytime conference music, panels and parties, plus priority access to all evening showcase performances and a ticket to the \ Americana Honors & Awards show. The general public can also purchase tickets for individual nightly showcases.
To register to attend the conference, visit www.americanamusic.org.


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Hi. We are taking a week long cruise to the Bahamas soon and we have a science project due soon after. Its for a 3rd grader. Any ideas of experiments we can easily do on the cruise ship or beaches of were we port? Florida, key west, and Bahamas. We can collect sand from each port but what to do with it?
Any project ideas for us? Thank you


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Gordon and Brown on Thursday. (Christopher Polk/WireImage)
Although her rep tells CNN that Bobbi Kristina and 22-year-old Gordon are not headed down the aisle, they add, "You have to watch the show!" According to CNN, the young couple may have indeed been engaged when the scene was shot, but after her family's less-than-thrilled reaction, they changed their minds. In the clip, Pat Houston, the wife of Whitney's brother Gary, exclaims, "Engagement? This is unacceptable."
[Related: Young love in Hollywood (photos)]
Either way, it ignited a media firestorm for the couple and, of course, their upcoming reality show. So was it just a publicity stunt to get us to watch when the series premieres on October 24?
As for Bobbi Kristina and Gordon, they've remained mum on the exact definition of their relationship. On the teen's Twitter page, she constantly posts photos of them hugging and kissing and showing off the many tattoos they've gotten since Whitney's death in February.
[Related: Bobbi Kristina Brown and boyfriend involved in mysterious single-car accident: report]
And on Thursday night, the duo attended the "We Will Always Love You: A Grammy Salute to Whitney Houston" at the L.A.'s Nokia Theater — where Bobbi Kristina wore not one, but two diamond rings on her left ring finger.
More Celebrity Features on Yahoo!:
- Nick Lachey hopes son Camden becomes an athlete
- Rihanna goes topless on cover of latest album
- Lady Gaga makes rare public appearance with boyfriend, tweets kissing photo
- Arts & Entertainment
- Celebrities
- Bobbi Kristina Brown


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Recipe by MARBALET
"This is a great appetizer or side dish for your next get together."
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French baguettes, cut into 3/4 inch diagonal slices
1 large
minced onion
8 cloves
minced garlic
1/4 cup
2 cups
shredded mozzarella cheese
1/2 cup
grated Parmesan cheese
1 cup
This was delicious! It's an incredibly easy recipe. I cooked this for my friends at college in a dorm kitchen, so you know it's easy! I increased the ammount of cheese and I followed one reviewer's suggestion to only use 2/3 c. of mayo. The only problem was that there was not enough of the garlic mixture. We are garlic fiends! I would advise doubling that section of the recipe for more garlic deliciousness. I also added some oregano and pepper. For those of you wondering about the mayo, this recipe does not produce mayo-flavored garlic bread. You can only taste the mozzarella and garlic. Once again, a fantabulous recipe and this will be going in my recipe book. 15 college kids give this recipe a 4.0.
Not a bad recipe but if you are not a big fan of grilled onions you probably won't like this one too much. The grilled onion flavor really dominates this. Plus it is very fattening, a whole cup of mayo and a cube of butter, yikes! So I guess I'm still on the hunt for that really delicious cheesy bread recipe!
160 Ratings
Wonderful recipe! Although a tad greasy tasting for me,my family enjoyed t. The second time I made it, I cut the mayo to 2/3cup, and used finely shredded cheddar cheese (3/4c) in place of mozzarella.Thanks Holly.
I had to attend a party at my sisters and she put me in charge of garlic bread.
She said, "I am impressed, you pulled it off, this is excellent." There was not
one piece of this Cheese Onion Garlic Bread left. Everybody had three and four
pieces, they loved it so much. Try it you will love it. And it is easy, ask my
husband and son they made it. :~)
I am updating my rating and review to 5 stars. I just got back from a potluck. I brought the bread over all assembled except not broiled or cut. I used my neighbors over to broil and cut up the pieces of bread. I doubled the recipe and there was not a SINGLE piece of bread left! All our friends were raving about this bread.
The only change I made was that I caramelized the onions, cooked for about 45 minutes and then I added the garlic for 4-5 minutes at the end. Let the mixture cool and then spread it on the bread and topped with the cheese/mayo mixture. Perfect!
This bread is very good but has too much of a mayo taste for us. I will try this again for sure but cut the mayo in half, because the overall taste, again, to us was mainly mayo. The onion/garlic mix that's spread on before the cheese mix is great. I chopped the onion very fine and added crushed garlic to the butter mix and simmered about 5 minutes at medium low heat. Do not brown the onion mixture. The onion and garlic almost dissolved and became a part of the butter once cooled and it really is a wonderful spread. I will use this recipe again , but as written the mayo is very prominent. Thanks for sharing :)
This was excellent! I used Italian bread and it worked very well. I was out of mayo (which is a sin in this house), but it still tasted very good. It was easy to make, but watch it under the broiler. 5 minutes was too much in my oven and it was slightly black, but still yummy - thanks!
This is a terrific garlic bread recipe! I've been making garlic bread for years with our pasta dinners for my husband and I, but left the bread plain for the children. They do not usually enjoy garlic bread but LOVED this one. Just a few changes: Used half the amount of butter, added olive oil for the remainder, eliminated onion (not a favorite of the kids), crushed whole cloves of garlic and sauteed them until golden, then removed them. Oil/butter flavored nicely. (I put the garlic on my bread later, yuuummmm! Just don't stand too close!) Had Monterey Jack in the fridge instead of Mozzarella, worked very well. The cheese/mayo topping really makes this bread extra special. Way to go, Holly! - - - Virginia
* Percent Daily Values are based on a 2,000 calorie diet.
Cheese Onion Garlic Bread
Serving Size: 1/8 of a recipe
Servings Per Recipe: 8
Amount Per Serving
Calories: 687
Calories from Fat: 324
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text: 'Justice League: Doom' Gets First Promo Images And Voice Cast Details by Caleb Goellner September 27, 2011 If you were into the Justice League animated series, odds are you'll be stoked for Warner Home Video's animated adaptation of Mark Waid and Howard Porter's JLA "Tower of Babel" storyline, Justice League: Doom. TV Guide released the first promo image fo Read More Filed Under: Bruce Timm, BruceTimm, Bumper Robinson, BumperRobinson, Carl Lumbly, CarlLumbly, Dwayne McDuffie, DwayneMcduffie, justice league, Justice League Doom, JusticeLeague, JusticeLeagueDoom, Kevin Conroy, KevinConroy, Lauren Montgomery, LaurenMontgomery, Michael Rosenbaum, MichaelRosenbaum, nathan fillion, NathanFillion, Susan Eisenberg, SusanEisenberg, Tim Daly, TimDaly Category: Animation, DC, Movies Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


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About LIM College
Next Generation Awards Winners Announced
Three LIM College students win $2,500 each in Accessories Council competition
Samantha Vigorito, Meaghan O'Malley, and Kristine Lusardi are the winners of the Next Generation Awards competition, a program developed by the LIM College and the Accessories Council that focuses on cultivating the business skills of tomorrow's fashion industry leaders.
The students' $2,500 cash awards were presented at a breakfast at LIM College on September 24. The Next Generation competition required LIM College students to create a plan for a new fashion accessory product. Samantha (pictured above) won the "Innovative" category with a plan to create "Bring on the Rain," a line of rain boots, tote bags, and umbrellas which would utilize hydrochromatic ink to make artwork appear on these items when they got wet. Meaghan won in the "Small Leather Goods" category for a MetroCard holder, and Kristine's products made from recycled tires took top honors in the "Eco-Friendly" category.
The judges were:
Peggy Freeman, Director of Licensing - Amiee Lynn Accessory Company
Bob Conrad, Senior Vice President of Design and Product Development - Henri Bendel
Linda Lombardi, Vice President, Global Creative and Store Design - Godiva
Alexandra Patino, Director of Jewelry Design - Jones Jewelry Group
Lonna Conte, Design Director of Classic Brands - Jones Jewelry Group
Winners were selected based on a combination of elements, including an oral presentation, visual presentation materials, and the overall strength of their marketing concept.
Says LIM College President Elizabeth S. Marcuse, "The Next Generation Awards recognize the next wave of executive talent in the fashion industry. All of the participating students did a great job of synthesizing all of the fashion and business education and experience they've acquired so far at LIM College into one project."
About the Accessories Council
The Accessories Council is a not-for-profit, national advocacy organization whose mission is to stimulate consumer awareness and demand for fashion accessory products. Its membership includes more than 150 corporations representing the world's leading brand names, designers, publications, retailers and associated providers for the accessories, eyewear and footwear industries.


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In this section
Goa Education Trust Scholarships 2015
The Goa Education Trust (GET) Scholarship is an opportunity for Goans to pursue Masters in the UK.
Hornby Scholarships
The Trust supports the teachings of English as a foreign language in partnership with the British Council and by awarding scholarships to English language teachers from developing countries.
Commonwealth Scholarship and Fellowship Plan
This is an annual scheme made available to all Commonwealth countries by the Commonwealth Scholarships Commission. Applications for 2016 are now open.
Charles Wallace India Trust Scholarships
The Charles Wallace India Trust (CWIT) is an arts, heritage conservation and humanities scholarship fund.
Scholarships funded by other organisations
Students may apply for a range of scholarships and funding provided by UK and international Government bodies, the European Commission, trusts, research councils, industry and employers.


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Avery® Removable Multi-Use Labels 41448, Green Border, 3-3/4" x 1-5/8", Pack of 15Product Number: 41448
Getting your home organized has never looked so good. Our Removable Multi-Use Labels not only stick and stay put, but they remove cleanly when you need to make a change. Their arched rectangle shape and green border give a nice finishing touch to all your organization needs and they're perfect for use on glass jars or plastic storage bins. Easily personalize them with free software and templates at avery.com/print for good-looking organization that lasts.
Product Description:


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|The Old World
For as long as Saturn remained the boundary of the solar system, the Sun, Moon and five planets explained and defined the totality of human experience in a remarkably complete and comprehensive way. Symbolically, the number seven is a number of completion, harmony and balance. There are seven notes in the octave, seven colours in the rainbow, seven wonders of the world and seven 'ages of man', describing our expanding awareness as we grow to maturity:
All the world's a stage, and all the men and women merely players: they have their exits and their entrances; and one man in his time plays many parts, his acts being seven ages. [24]
The Planetary days
The seven days of the week are particularly significant astrologically, since each of the days in the week is named after one of the planets. The table below describes the derivation of the names of the days of the week.
What is interesting about the seven-pointed star above is that, if you follow the arrows from planet to planet, they describe the order of the days of the week. In addition to this, the planets around the outside of the star follow the ancient 'Chaldean' order of the planets, based on their increasing speed from the point of view of the earth: Saturn, Jupiter, Mars, Sun, Venus Mercury Moon. You may even remember the traditional nursery rhyme describing the characteristics of people born on each day of the week:
The point about this is that for thousands of years astrology has been based on the idea of seven as the number of completion. Saturn represented the ultimate temporal authority, the 'ring pass not' of the solar system and of the psyche. The discovery of the three outer planets and of Chiron has catapulted us into a very new world indeed, and we will look at these next.
"Mapping the Psyche"First published 2005 by the CPA Press, BCM Box 1815, London WC1N 3XX, United Kingdom, www.cpalondon.com.
Copyright ©2005 by Clare Martin.
More Information about the Book.
Mapping the Psyche, by Clare Martin
Order at Midheaven Bookshop
Please note: Mapping the Psyche, vol. I, is currently out of print.


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If you can dream it, you can do it. -Walt Disney
Quality is a great business plan. -John Lasseter
Let's make some funny pictures. -Tex Avery
I never considered a difference of opinion in politics, in religion, in philosophy, as cause for withdrawing from a friend. -Howard Zinn
When critics sit in judgment it is hard to tell where justice leaves off and vengeance begins. -Chuck Jones
And what do you benefit if you gain the whole world but lose your own soul? -Jesus
A man should never neglect his family for business. -Walt Disney
What's most important in animation is the emotions and the ideas being portrayed. -Ralph Bakshi
Once you have heard a strange audience burst into laughter at a film you directed, you realize what the word joy is all about. -Chuck Jones
Before enlightenment: chop wood, carry water. After enlightenment: chop wood, carry water. -Buddhist Proverb
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Showcase your drawings, illustrations, video and media.
5 posts • Page 1 of 1
Heres the beginning of a NEW ERA.
Specifically located in the city of Des Melonios, Iowa, these characters are caught in the middle of there once normal lives.
There is a strange reality in Des Melonios and is waiting to be witnessed by outsiders. This reality is called the Ality, and it is very unreal.
We welcome you to join as more and more stories are developed.
I am neither here nor there. Our company was started from notes on a paper plate collection and evolved into lined paper and a computer. Des Melonios was not a byproduct of the public education and has no influence from it. Self produced, self composed, self inducted Quality Entertainment.
Our Goal is to push "Product" out the Door as fast as we can.
"Product" is defined in the state of Iowa as anything that is Complete enough to give to somebody, Which is why you can purchase disassembled Furniture.
Please take the time to subscribe to Our Page to stay up to date with future uploads.
Thank you and have a Day.
Re: THE FALKEN SHOWCASE
Des Melonios. Interesting place. Sounds like a cross between Des Moines, Iowa and Moline, Illinois.
Welcome to AN and thanks for posting a link to your films.
Daily Z
Re: THE FALKEN SHOWCASE
Des MElonios: The Melon
A tribute to SCTVs home, Melonville.
Iowa: fun to say.
Re: THE FALKEN SHOWCASE
Growing up in the Midwest I projected places that I knew to the names. Especially in regards to Iowa. Thanks for the explanation.
Daily Z
Re: THE FALKEN SHOWCASE
Iowa you're welcomes yourself.
5 posts • Page 1 of 1


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Washington University in St Louis Olin Business School
Compare the options that best fit your needs.
The Master of Science in Supply Chain Management (MSSCM) degree at Washington University's Olin Business School equips graduates to stand out in this challenging and critically important career field.
Victor talks about the MSSCM program. This intensive 36-credit hour program brings together faculty renowned for their work in risk management and supply chain research, a cutting-edge curriculum, and industry collaboration to create one of the most sought-after supply chain management degrees in the nation.
The MSSCM program is typically completed in three semesters, with the option to complete the program within two semesters. Working professionals in the St. Louis area can pursue the program on a part-time basis. Students will:
Learn more about our MSSCM degree:
Our admissions team is travelling with a consortium - Specialized Programs in Graduate Business - that hosts forums for individuals interested in focused degrees such as accounting, finance, supply chain management. View upcoming events.
Follow Olin


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Some product exclusions may apply. See product page details for more information. Clearance items are final sale. Price matching does not apply to this or any promotion. Offer is valid until September 13 at 11:59 PM PST. May not be combined with any other offer/discount (unless specified), including invitation credits, promotional gift cards and/or special discount links. Discount applied upon checkout. Log in to your account to see your savings.
As soon as sustainable design innovator Yves Béhar caught wind of SodaStream's environmentally conscious home soda maker, he had to put his creative spin on it. Featuring an earthy beech wood finish, the pared-down Source Soda Maker reflects Béhar's love of high-tech solutions with a human element. Simply fill the carbonating vessel with tap water, lock it into place, and press the lid to release a jet of bubbles within. Powered by a CO2 canister, this device saves about 550 plastic bottles a year.
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Featured Products
Item # 23499
Item # PENSL12BP5M
Item # SHF73404
Item # 24436_CASE
TheRoyalStore.com is a paper store for all your home, school and office paper products needs. We are also one of the oldest paper coaters in the USA and we manufacture our own Photo Paper - Royal Brites. Royal Brites Poster board is a patented product - the original 2 Cool Colors concept -poster board with a different color on each side, saves you space and money. Look for Royla Brites Neon Board, also called Fluorescent Poster Board, Primary Colors Posterboard, Grid Board and more.
Our 100 year old Paper Doily business supplies the most beautiful Royal Lace Doilies and Placemats on the market. These unique paper doilies have authentic royal lace patterns from European museums. All our Doilies and Placemats are made with hand-crafted metal dies, which makes them unique in the world.
Since we have been in this business for decades, we have a good understanding of the various paper products you may need for your home, school, office or retail store.
We mainly specialize in poster boards and photo paper that are widely used in home, school and business. If you are running a promotional activity in your store, or announce a sale event at hope, Royal Brites poster boards are an ideal choice. We have poster board in various sizes and colors on sale.
Neon boards are the most preferred form of advertising for any business, today. Whether you advertize a tag sale or a school event or want to grab the attention of the passers-by to a promotion in your store or a new event, your potential customers will notice the Royal Brites Poster Board easily. We manufacture neon poster boards in three main sizes sizes in primary colors, two cool colors, fluorescent neon colors or metallic colors to suit your preferences. We also supply Retail signboards and Business signs.
Theroyalstore supplies all other Royal paper products such as Royal Lace Paper doilies, paper placemats in retail Packs and Food Service or bulk qualtities. To complement our Royal Lace Food Service line, we offer white cardboard dinner boxes and menu design paper.
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Why should you shop for paper products at www.TheRoyalStore.com?
- Extensive range of US Made Paper products
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Recently, New York Governor Andrew Cuomo made a clear statement that the good people of New York who hold to a pro-life position are not welcome in his state. During an interview last Friday, Cuomo, commenting on problems within the Republican party, said, "Their problem is not me and the Democrats; their problem is themselves. Who are they? Are they these extreme conservatives who are right-to-life, pro-assault-weapon, anti-gay? Is that who they are? Because if that's who they are and they're t … [Read more...]
Children-Loving New Yorkers Move To Nebraska
January 22, 2014 by Leave a Comment


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92nd Street Y is where inspiration lives and exploration begins—with a diverse range of classes and programs that let everyone pursue what they love. With innovative, top-notch programs, events and concerts, plus over 4,000 classes taught by today's most talented professionals, 92Y has it all—for all ages and interests.
Events range from literary readings, concerts, lectures, single mixers, events, films, and many more.
From people who inspire a world of ideas to entertainers known the world over, find the best minds, brightest stars and most compelling issues at 92Y!
Where the written word comes to life, giving New Yorkers a literary forum unlike any other.
Learn more about the Unterberg Poetry Center
Music that puts you on the edge of your seat.
Celebrate dance through electrifying performances featuring today's masters as well as emerging new talent.
Take your passion for the performing arts even further.
Learn about the
Ceramics Program
Jewelry Center
Fine Art & Design Program
Learn about the School of Music
Learn about the Harkness Dance Center
Learn about the Unterberg Poetry Center
Our state-of-the-art complex is where New Yorkers find healthy inspiration. With our 25-yard lap pool, 100 weekly classes, plus expert training and services, we make fitness fun, flexible and fresh.
Learn More
Join the Health, Fitness & Sport eNews
One of the most widely acclaimed Jewish programs in the nation. You'll find a wealth of programs designed to inform and inspire a meaningful connection to Jewish beliefs, teachings, traditions and celebrations.
Learn more about the Bronfman Center for Jewish Life
A welcoming and well-rounded resource, providing Jewish education and shared family experiences that engage children of all ages.
92Y is a special place to celebrate Jewish holidays and days of remembrance.
Offering a broad range of services and outreach programs to facilitate personal development, and to help Jewish adults build community connections.
Where parents and children come to learn, explore, discover and celebrate through programs and shared experiences that embrace every age, and every life stage.
No performances are currently available.
How is leadership defined in an era in which technology, entrepreneurism and social good are growing increasingly linked? How are the most forward-thinking leaders combining enduring values with innovation and change in a rapidly changing world?
Can't make it to the event? Leave your questions for our guests below, and they might be used on stage during the Q&A. Keep an eye on 92Y On Demand after the event for any video clips we might share! You might see your question used on stage.
This series is presented in partnership with the Harvard Kennedy School's Center for Public Leadership.
Jared Cohen is the founder and Director of Google Ideas, an Adjunct Senior Fellow at the Council on Foreign Relations and the coauthor with Eric Schmidt of The New York Times Bestseller The New Digital Age: Reshaping the Future of People, Nations, and Business. Previously he served as a member of the Secretary of State's Policy Planning Staff and as a close advisor to both Condoleezza Rice and Hillary Clinton.
Cheryl Dorsey is President of Echoing Green, a pioneer in the social entrepreneurship movement. This global social venture fund has awarded over $30 million in start-up capital to over 500 next generation social entrepreneurs worldwide since 1987. Dorsey received an Echoing Green Fellowship in 1992 to help launch The Family Van, a community-based mobile health unit in Boston.
Jack Andraka is an inventor, scientist and cancer researcher. He is the recipient of the 2012 Gordon E. Moore Award, the grand prize of the Intel International Science and Engineering Fair. Andraka was awarded the $75,000 Award for his work in developing a new, rapid and inexpensive method to detect an increase of a protein that indicates the presence of pancreatic, ovarian and lung cancer during early stages when there is a better survival rate with current treatments.
David Gergen is a professor of public service and director of the Center for Public Leadership at the Harvard Kennedy School, as well as a senior political analyst for CNN. He has served as a White House adviser to four US presidents.
If you have any questions, need assistance with your order or require special seating considerations, such as wheelchair accessible seating or hearing assistance, please call Customer Service at 212.415.5500 during our Hours of Operation.
If you prefer, you can order your tickets and class enrollments by calling Customer Service at 212.415.5500 during our Hours of Operation, using Visa, MasterCard or American Express. You can also place your order by fax, by mail, or in person at our Box Office on Lexington Avenue at 92nd Street.
Groups of 10 or more receive a 15% discount. Please note that certain events may not qualify for a group rate. To make group arrangements or need further assistance, you may contact Customer Service at 212.415.5500 during our Hours of Operation.
92Y YourStage provides a venue for independent curators, performers, and educators to mount a professional production. Yourstage events are confirmed once they meet a threshold for ticket sales by a certain date.
YourStage events that are ON have been confirmed; PENDING events need to generate more ticket sales; If an event fails to generate enough ticket sales, the event will be CALLED OFF, and all ticket holders will be refunded.
Individuals who have participated in 92nd Street Y programs over the past year in selected program areas, participants in certain memberships, and those who have made contributions of $500 or more to 92Y, are eligible to register for programs before they become available to the general public.
Patrons that qualify for Priority Registration will receive packets in the mail explaining how to purchase online. Priority registration is normally mailed 2-3 weeks before a catalog is available. Registration information includes your Patron ID#. You can use this ID# to setup your login information online. This will allow you to register early for a course or event. Please note: if you receive a packet, you are only eligible to priority register for the programs covered in your packet.
To find out if you are eligible for priority registration, don't have your Patron ID#, or having difficulty ordering online, please call 212.415.5500 or email.


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Perkins Schools is set to approve a slew of cuts Wednesday totaling $12 million over the next few years.
The list of district-wide reductions, unveiled late last month, includes axing nearly 40 jobs, eliminating field trips and at least doubling student pay-to-participate athletic fees. About 25 employees will be directly impacted, superintendent Jim Gunner said.
School board approval will make the changes final, for now, although the board plans to divide the cuts into two rounds.
The first round is larger and permanent. It totals about $2.4 million in the coming school year and $3.5 million the following year.
The second round, including fee increases and field trips, can be reversed if a majority of voters favor the district's May levies. It totals about $468,000 in the upcoming school year.
The district is proposing two issues on the May ballot:
* A five-year renewal of a 2-mill permanent improvement levy.
* A 10-year, 4.98-mill emergency operating levy.
"If and when the levy passes, we'll bring them back as quickly as possible, based upon the finances of the district at that time," Gunner said after announcing the cuts in February.
If the 10-year levy fails, the district would face a deficit of more than $600,000 heading into 2014, with an annual budget of about $23 million.
View a list of the reductions here.


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Guns N' Roses, Rock and Roll Hall of Fame Induction: Band Reunites — Without Axl Rose — in Cleveland
CLEVELAND (AP) -- On their way into the Rock and Roll Hall of Fame, Guns N' Roses got together for one more gig.
Axl Rose missed it.
The hedonistic hard rockers, who became the world's top music act amid endless dysfuncti