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Antifungal Single Agent - Mammalian Cell Toxicity Measured in Cell-Based and Microorganism Combination System Using Plate Reader - 2148-03_Inhibitor_Dose_CherryPick_Activity
Assay Overview: The basic assay strategy will consist of NIH 3T3 mammalian fibroblasts cultured in 384-well format. Test compounds that do not inhibit subsequent growth will merit further evaluation for their selectivity for fungal inhibition over mammalian cell toxicity. This whole cell phenotypic screening approach will only capture compounds that are capable of entering and accumulating in more ..
BioActive Compounds: 35
Related Experiments
Keywords: Candida albicans, drug resistance
Assay Overview: The basic assay strategy will consist of NIH 3T3 mammalian fibroblasts cultured in 384-well format. Test compounds that do not inhibit subsequent growth will merit further evaluation for their selectivity for fungal inhibition over mammalian cell toxicity. This whole cell phenotypic screening approach will only capture compounds that are capable of entering and accumulating in cells to bioactive concentrations. Compound activity will be measured by the metabolism of Alamar Blue, a cell stain that is metabolized to a fluorescent product by living cells but that remains non-fluorescent in wells with growth-inhibited organisms. Geldanamycin serves as the inhibitory control.
Expected Outcome: Wells in which there is toxicity will show a reduced fluorescence intensity due to a reduction in the amount of Alamar Blue dye metabolized by fewer viable cells.
Stock solutions
Geldanamycin (GA) 15mM stock sol'n in DMSO
Pen/Strep 100x in PBS
Assay Medium:
Optimem medium (Invitrogen Cat#31985-070, Lot# 548536)
2.5% (v/v) Fetal Bovine Serum (Hyclone Cat#30071.03, Lot# ARF26748)
1% (v/v) Pen/Strep solution (Invitrogen Cat#15140-122, Lot#529891)
Cell Inoculum
Test Strain: NIH-3T3 mammalian fibroblasts (ATCC # CRL1658)
Concentration in assay plate:#Cells plated at 1,000/well in 40 uL assay medium and cultured overnight at 37C under 5% CO2 in 384-well, transparent bottom, black, tissue culture-treated, bar-coded assay plates
Procedure:#After overnight culture, compounds are pinned into wells at 100nL/well using the CyBio pinning instrument. The final nominal concentration in the well will be a dose range of 26 - 0.195 uM of test compound. The plates will be returned to the tissue culture incubator and culture continued for 48 hrs at 37C under 5% CO2. At the completion of this incubation, Alamar Blue solution diluted 1:40 will be added to each well (10uL/well) to achieve a final dilution of 1:200. Plates will be incubated for a further 2-3 hrs at 37C under 5% CO2 and then fluorescence intensity as a measure of relative viable cell number will be determined on an Envision plate reading fluorometer.
Dilute Alamar Blue Reagent (Biosource International Cat# DAL1100) 1:40 in Ca/Mg-free PBS. Add 10ul/well to plates with Combi (final dilution factor 1:200). Incubate 2 hrs at RT. Read Relative Fluorescence Intensity (RFU) of wells on standard plate reader as measure of relative fungal growth.
Plate reader set-up- Envision, Perkin Elmer: Ex 544nm, Em 590nm, Bandwidth 12nm, Top read.
PRESENCE OF CONTROLS: Neutral control wells (NC; n=36) and positive control wells (PC; n=36) were included on every plate.
EXPECTED OUTCOME: Active compounds result in decreasing readout signal.
For each sample, the highest valid tested concentration (Max_Concentration) was determined and the active concentration limit (AC_limit) was set to equal (2)(Max_Concentration).
The raw signals of the plate wells were normalized using the 'Neutral Controls Minus Inhibitors' method in Genedata Assay Analyzer (v7.0.3):
The median raw signal of the intraplate neutral control wells was set to a normalized activity value of 0.
The median raw signal of the intraplate positive control wells was set to a normalized activity value of -100.
Experimental wells values were scaled to this range.
PATTERN CORRECTION: The plate pattern correction algorithm 'Runwise Pattern (Multiplicative)' in Genedata (v7.0.3) was applied to the normalized plate data.
AC values were calculated using the curve fitting strategies in Genedata Screener Condoseo (7.0.3).
AC values were calculated up to the active concentration limit described for each sample.
pAC was set to equal -1*log10(AC)
Activity_Outcome = 1 (inactive) when:
a) compound shows activity but in a direction opposite to the expected outcome
in these cases, values describing curve fitting parameters (Sinf, S0, Hill Slope, log_AC50, log_AC50_SE) are set to null
b) curve fit is constant where activity is > -30% and < 30% at all tested concentrations, or
c) AC > AC_limit
Activity_Outcome = 2 (active) when:
AC <= AC_limit
Activity_Outcome = 3 (inconclusive) when:
a) Curve fitting strategy resulted in a constant fit with activity >= -70% but <= -30%, or
b) The fit was deemed not valid due to poor fit quality.
If PUBCHEM_ACTIVITY_OUTCOME = 1 (inactive) or 3 (inconclusive),
then PUBCHEM_ACTIVITY_SCORE = 0
If PUBCHEM_ACTIVITY_OUTCOME = 2 (active)
then PUBCHEM_ACTIVITY_SCORE = (10)(pAC)
Scores relate to AC in this manner:
120 = 1 pM
90 = 1 nM
60 = 1 uM
30 = 1 mM
When the active concentration (AC) is calculated to be greater than the highest valid tested concentration (Max_Concentration), the PUBCHEM_ACTIVITY_SCORE is calculated using Max_Concentration as the basis.
When the active concentration (AC) is calculated to be less than the lowest tested concentration, the PUBCHEM_ACTIVITY_SCORE is calculated using the lowest tested concentration as the basis.
The individual dose data point columns ('Activity_at_xxuM') reported here represent the median of valid (unmasked) replicate observations at each concentration. These values are the inputs to a curve fitting algorithm.
All other data columns represent values which are derived during the curve fitting algorithm; this may sometimes include automatic further masking of some replicate data points.
Occasionally this results in perceived inconsistencies: for example, between the derived 'Maximal_Activity' and the apparent most active data point.
Categorized Comment - additional comments and annotations
From PubChem:
Assay Format: Cell-based/Organism-based
Assay Type: Toxicity
Assay Cell Type: NIH 3T3
Result Definitions
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: 1 R03 MH086456-01
Data Table (Concise)


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When: Wednesday, March 28, 2012
6:00 p.m. - 8:00 p.m.
Loews Philadelphia Hotel
1200 Market Street
Philadelphia, PA, PA 19107
Download the podcast from this program.
Our March community meeting featured guest speaker Sabitha Pillai-Friedman, PhD, LCSW, CST. At this program, Dr. Pillai-Friedman shared information on the intimate challenges faced by women affected by breast cancer of all ages and relationship statuses, including tips on:
- Managing body image concerns
- Interventions to enhance physical comfort and to increase libido
- Talking to your partner about what you are experiencing
About Our Speaker
Sabitha Pillai-Friedman, PhD, LCSW, CST, is an assistant professor at the human sexuality program at Widener University. She is a psychotherapist and certified sex therapist who works with individuals and couples in her private practices in Center City, Philadelphia and Bala Cynwyd, Penn. She has over 20 years of experience in providing individual, couple and sex therapy.
Dr. Pillai-Friedman presents nationally and internationally on various topics related to relationships and sexuality and has done interviews on CN8, NBC and National Public Radio.
You may also be interested in our March 2012 Ask the Expert session on Sex and Intimacy.

subset: commoncrawl


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Sociological Methodology
Editor: Tim Futing Liao, University of Illinois
Annual: August
Mission Statement
Online Access
Editorial Board
Reprint Permissions
Author's Corner
• ASA Style Guide
Sociological Methodology (SM) is the only American Sociological Association periodical publication devoted entirely to research methods. It is a compendium of new and sometimes controversial advances in social science methodology. Contributions come from diverse areas and have something new and useful—and sometimes surprising--to say about a wide range of methodological topics. SM seeks qualitative and quantitative contributions that address the full range of methodological problems confronted by empirical research in the social sciences, including conceptualization, data analysis, data collection, measurement, modeling, and research design. The journal provides a forum for engaging the philosophical issues that underpin sociological research. Papers published in SM are original methodological contributions including new methodological developments, reviews or illustrations of recent developments that provide new methodological insights, and critical evaluative discussions of research practices and traditions. SM encourages the inclusion of applications to real-world sociological data. SM is published annually as an edited, hardbound book.
Featured Article
The Effect of Labeling and Numbering of Response Scales on the Likelihood of Response Bias by Guy Moors, Natalia D. Kieruj, and Jeroen K. Vermunt (2014)
Modeling Household Decisions Using Longitudinal Data from Household Panel Surveys,with Applications to Residential Mobility by Susanne Vogl (2013)
Ways of Measuring Agency: An Application of Quantitative Narrative Analysis to Lynchings in Georgia (1875-1930) by Roberto Franzosi, Gianluca De Fazio, and Stefania Vicari (2012)


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Pan Am Vintage-Style 25" Trolley Rolling Suitcase
Pan Am Luggage is a sure find at baggage claim!
This fashion piece is a sure find on the airport conveyor belt.The Globe Print Collection by Pan Am succeeds in transforming our world famous logo into a fun, funky pattern that makes Pan Am just as cool and relevant today as it was fifty years ago. This 25" case leaves ample room for all your checked baggage needs.
25" Trolley features:
Pan Am Vintage-Style 25" Trolley Rolling Suitcase
Shopping Cart


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Jack Stoddart came to rural Overton County, Tenn. back in the early '70s and established a homestead where he and his family could connect with the land.
These days the renowned photographer has opened his spread for the Jammin at Hippie Jack's 2010 Memorial Day Festival, a multi-day celebration of several strains of American roots music.
Yes, there's camping, but Bonnaroo this ain't — cell phones don't work here, and the atmosphere is geared toward folks of all ages who like hearing good music in the open air. Among the performers this year are irrepressible roots rocker Webb Wilder, hard-bitten troubadour Malcolm Holcombe and long-lived folk-rockers Goose Creek Symphony.
The festival is May 28-30 at Stoddart's farm near Livingston, Tenn. For directions, ticket packages and schedules visit www.myhippiejack.com.


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Alex Berezow and Stephen Meyer talk about God and Evolution on the Michael Medved Show
"I believe that demonstrating the existence of intelligent agency with the tools of science is impossible." - Alex Berezow
The Resource Finder has powerful filtering features — check out the tutorial to learn more.
"I believe that demonstrating the existence of intelligent agency with the tools of science is impossible." - Alex Berezow
In a recent book review published in Christian Century, BioLogos content manager Jim Stump endeavors to reclaim the word "design."
We, both in the church and in broader society, need to think carefully together about how new technology should be used, even as we give thanks for the fruitfulness of modern science, medicine, and technology.
Jim Stump explores the meaning of the Lenten season from the perspective of evolutionary creation.
Here are some of the best responses from around the web to David's Barash's controversial editorial about God and Evolution in the New York Times.
If discussions of science and religion sometimes get bogged down in Genesis, perhaps that is because they have not made the preparatory journey through the rich material of the Wisdom books.
Ted Davis traces the interesting (and often disturbing) history of how Christians have used evolutionary science to justify un-Christian views, and makes some suggestions about a better relationship between science and Christian faith.
This weeks in Origins news: studies of a skeleton uncovered in 2007 provide clues about rapid human evolution in the Americas, our climate and Christian stewardship, a few BioLogos folks give interviews, and some cool links to miscellaneous finds.
We must press beyond the various creation narratives in the Hebrew Bible, including the final chapters of Job, to the picture of God revealed in the New Testament—the Creator who does not rationally explain away the scandal of suffering but who instead enters into it.
This week in our Origins News Roundup: News of new nucleobases, the future of synthetic biology, and some healthy dialogue and critique of certain aspects of modern science.
Today's Origins News Roundup features a tour of trending topics in neuroscience, from commentaries and questions made recently by popular media to developments in neuroengineering and the BRAIN initiative.
The BioLogos Book Club discussion of Francis Collins' The Language of God.
Sometimes, out of fear or some sense of being required to defend our position, Church leaders and teachers have hastily set up a boundary marker around some doctrinal theory which they have confused as a core doctrinal issue.
"I believe it is fair to conclude that the supposed threat posed to human free will and moral responsibility from the very much work-in-progress social and cognitive human sciences is quite overblown."
"It's tempting to think that the church needs to decide quickly which of these [original sin] scenarios is right, and which ones must be wrong. I believe the church is better served by taking its time, holding several different scenarios in tension for a while as we think through the implications of each."
A deep love for scripture, coupled (ironically) with a lifelong struggle with religious doubt, led Robert Boyle to write several important books relating scientific and religious knowledge. We explore aspects of this fascinating interaction.
Critics of Christianity look to evolution to show how the emergence of human life on earth demanded enormous ruin and ravage, billions of years of apparent waste and futility, species extermination and organism road kill. Not only was the massive dying off rampant, it's mandatory too.


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COUNCIL BLUFFS — Creston/O-M handed Lewis Central its first dual wrestling loss of the season in convincing fashion Tuesday night, 54-18.
The Panthers, now 6-1 in the Hawkeye 10 and 9-1 overall, also rolled past Carroll Kuemper, 55-18. LC, now 3-1 overall and in the league, defeated Kuemper but the score was not available at press time. Glenwood continues to lead the Hawkeye 10 at 7-0.
Kuemper fell to 0-7 and 3-14.
Lewis Central was missing one of its best wrestlers at 195 pounds, but that is where the Panthers have No. 1-ranked Keaton Hulett, so the Titans' loss didn't have much effect on the outcome, Panther coach Darrell Frain said.
For the Panthers, Cole Tanner returned to the lineup at 220 pounds and secured a fall in 3:49.
"We got a lot of the close matches to go our way, and we got them on their back for (four) pins," Frain said.
Gage Cheers, Joey Huntington, Wyatt Thompson and Tanner had falls for the Panthers. Trevor Frain and Jake Marlin had technical falls, and major decisions were posted by Stone Hammons and Jacob Johnston.
Marlin's 17-2 technical fall was noteworthy in that it came at 152 pounds, after weighing in at 145, against LC's Ethan Ruby, who recently moved down from wrestling at 170 and 160 pounds. Marlin, three-time state champion, is ranked No. 1 at 138.
"That kid was a physical kid coming down from 160," Frain said. "Jake got takedowns and tilted him a bunch in the third period."
Marlin had a 20-second fall in the Kuemper dual.
LC's second-ranked heavyweight, Zach Renshaw, pinned Nathan Robinson, and two of the top Titans won at 160 and 182 pounds.
"They're just not as good in the bottom half of the lineup and we controlled those matches," Frain said.
Frain said the Kuemper match went according to form, with a bonus of an overtime 9-4 win at 152 by Gavin Leith against Kolby Hilgenberg,
"Hilgenberg has been ranked, so that's a good win for Leith," Frain said.
Kuemper's Alexander Schmitz, ranked ninth in Class 1A, beat Gage Cheers 8-2 at 126 pounds.
The Panthers figure to see some strong competition at the Centerville Invitational Saturday, beginning at 10 a.m.
Creston/O-M 54, Lewis Central 18
220 — Cole Tanner (Cr) pinned Riley Blay (LC), 3:49.
285 — Zach Renshaw (LC) pinned Nathan Robinson (Cr), :17.
106 —Stone Hammons (Cr) major dec. Daytona Smith (LC), 13-1.
113 — Wyatt Thompson (Cr) pinned Nash Matrell (LC), :33.
120 — Kruz Adamson (Cr) by forfeit.
126 — Gage Cheers (Cr) pinned Jordan Bailey (LC), 1:33.
132 — Joey Huntington (Cr) pinned Hayden Furler (LC), 2:46.
138 — Mason Heredia-Pugh (LC) dec. Zack Peppmeier (Cr), 7-3.
145 — Jacob Johnston (Cr) major dec. Nick Ruis (LC), 12-4.
152 — Jake Marlin (Cr) tech. fall Ethan Ruby (LC), 17-2.
160 — Ryan Nightser (LC) dec. Adam Baker (Cr), 4-0.
170 — Trevor Frain (Cr) tech. fall Hayden Walstein (LC), 15-0.
182 — Austin Lear (LC) pinned Seth Maitlen (Cr), 1:32.
195 — Keaton Hulett (Cr) by forfeit.
Creston/O-M 55, Kuemper Catholic 18
182 — Seth Maitlen (Cr) pinned Mitch Rohe (CK), :33.
195 — Seth Gehling (CK) pinned Zach Hoffman (Cr), 3:29.
220 — Keaton Hulett (Cr) by forfeit.
285 — Nathan Robinson (Cr) pinned Alex Bruch (CK), 1:02.
106 — Brandon Gross (CK) dec. Stone Hammons (Cr), 8-2.
113 — Wyatt Thompson (Cr) by forfeit.
120 — Kruz Adamson (Cr) pinned Andrew Irlbeck (CK), 1:34.
126 — Alexander Schmitz (CK) dec. Gage Cheers (Cr), 8-2.
132 — Dylan Dunn (CK) pinned Joey Huntington (Cr), 3:04.
138 — Zack Peppmeier (Cr) pinned Austin King (CK), 1:06.
145 — Jake Marlin (Cr) pinned Brett Sibbel (CK), :20.
152 — Gavin Leith (Cr) dec. Kolby Hilgenberg (CK), 9-4 OT.
160 — Adam Baker (Cr) major dec. Adam Scharfenkamp (CK),11-2.
170 — Trevor Frain (Cr) by forfeit.
Junior varsity
126 — Jared Rotert (CK) pinned Jake Routh (Cr), 2:57. 138 — Trey Jorgensen (CK) dec. Cameron Leith (Cr), 6-4. 120 — Alex Fargo (Cr) pinned Nicholas Wanninger (CK), 2:26. 138 — Tyler Helm (Cr) pinned Schuyler Murray (LC), :58. 132 — Bryce Briley (Cr) pinned Mason George (LC), :31. 138 — Garret Taylor (Cr) pinned Austin King (CK), :59. 160 — Tayler Pettit (Cr) pinned Isaack Anderson (CK), :59. 138 — Cameron Leith (Cr) pinned Jared Nelson (LC), 3:26.
152 — Jesse Rathe (Cr) major dec. Cory Lear (LC), 15-4. 106 — Paxton Whiteaker (LC) pinned Katie Stevens (Cr), 1:04. 132 — Garret Taylor (Cr) pinned Jared Nelson (LC), 1:57. 138 — Bryce Briley (Cr) pinned Schuyler Murray (LC), :28. 195 — Zach Hoffman (Cr) pinned Jake Mutum (LC), 1:47. 120 — Alex Fargo (Cr) pinned Mason George (LC), 1:28.


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I'm a right-front-pocket type but I hate it when my iPhone slips down to the bottom of my pocket and sits horizontally across my leg and it's worse (all bunched up) when I sit down.
I know, it's not the end of the world, but I decided to do something about it.
Step 1: Where the iPhone wants to go
i think this will work for most phones
I guess the two rivets on the watch pocket could scratch it when you put it in or take it out. If you were worried, you could do the mod in your left pocket (no watch pocket on that side).
Perhaps a pocket in your pocket, or a thin velcro strip sewn, or stuck down the middle would be even better.
What I hate the most is when you are driving and you can't get to it because its in your pocket with your seat belt over that. Perhaps a pocket sewn mid thigh to the side would work even better. Let us know how that goes?
You'd be surprised how short a seam is necessary to keep the phone upright. I reckon an inch might do it, which would make the rest of the pocket usable for all but those with severely webbed digits.
P.S. I have a blackberry
I carry quite a lot in my pockets and am rather annoyed when all the articles bunch up at the bottom (quite unsightly and uncomfortable) this should fix the issue nicely!
I posted two instructables yesterday.
This one took a half hour to do from start to finish. The other one took half a day to make and an hour to write up.
Today, this one has 4,000 views and the other one only has 400.
I feel like a soap opera actor that just won a daytime emmy award.
I'd like to say to everyone here. You may have just witnessed the peak of my digital life. Now I can settle in for the long ride to internet obscurity.
This is a great instructable because it's easy and solves a very common minor irritation. And you Kept It Simple S____d!
Might even be worth sticking a rivet there!
Both ends of the rivet would be on the outside of the fabric, and the phone would be on the inside. The only way it would scratch the phone would be if the edge of the rivet wore through the fabric... which, granted, it might do after prolonged use.
Eh, probably not worth the risk, though. I formally retract the notion of a rivet! ;>)
Brava, thank you. :)


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- Red Bull USA - Sports & Entertainment News | Teams & Athletes
- Music & Entertainment
- Art
- Photos
- Wynwood Walls in Miami 2012
- Kenny Scharf mural at the Wynwood Walls
Image Details
- Views
- Date
- Dec 6, 2012
- Photographer
- Participants
- Events
- Copyright
- Tamara Warren/Red Bull Media House
- About this Image
- When developer Tony Goldman founded the Wynwood Walls in 2009, he was seeking to beautify an industrial section of Miami, tapping into the talents of both post-graffiti artists and street artists. Soon the Wynwood Walls took on a life of its own. It is a colorful, textured assemblage of murals and painted doors, including major works by Phase II, Futura, Swoon, Barry McGee and Kenny Scharf that has transformed the area into a colorful stretch of NW 2nd Avenue.
Related Content
Red Bull Curates selects two SF artists for Art Basel Feb 6, 2013
The Canvas Cooler Project kicked off its new season and selected two emerging artists to show at the SCOPE Art Fair in December.
The party's over, but the street art remains Dec 10, 2012
The glamorous Art Basel fair ended Sunday, but the street art murals known as the Wynwood Walls will remain on display all year round. Here are some highlights.
When night falls, Art Basel becomes Party Basel Dec 9, 2012
Naturally, the world's biggest art extravaganza would be accompanied by suitably sized parties. Here's a peek at our favorite moments.
Miami Bound! 4 NYC Artists Punch Tickets to Art Basel Sep 14, 2012
At the Red Bull Curates opening party in Brooklyn, four emerging street artists were picked by judges and the audience to show at the SCOPE Miami show in December.
Road to Art Basel: Red Bull Curates Hits NYC Sep 7, 2012
Red Bull Curates hits New York City as 20 emerging artists compete for a chance to show their work at the Scope art fair during Art Basel in Miami Beach.
Fighting the Gray Jul 11, 2012
New Zealand graffiti writer Askew One talks to us about getting back to basics, how social and cultural issues enter his work and fighting the forces of gray in his native Auckland.


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About LIM College
David Wolfe, member of LIM College's Fashion Industry Advisory Board, to lecture on "The Next 12 Years"
Don't miss this opportunity to hear from one of the fashion industry's most respected forecasters
David Wolfe, a highly respected fashion, color, and trend forecaster and a Creative Director with the Doneger Group, will give a presentation on "The Next 12 Years" on Monday, November 15th in FashionOpolis. In this lecture, Mr. Wolfe, who is known for his entertaining public speaking style, will share his thoughts on the future of fashion.
Seats are on a first-come, first-served basis, and the lecture will be presented at 11:40am, 2:30pm, and 4:20pm.


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|UCLA Football Tickets
2015 UCLA Football Season Tickets
Season tickets are still available! Get the best seats in the Rose Bowl at a discounted rate, plus a number of other great benefits, when you purchase a 5-game season ticket. Click the links below to view exact seat locations and order your seats today!
Or Call 310-206-5991
Click map to enlarge
UCLA Students - Den Sports Pass $169
Recent Alumni - Undergrad '11-'15, Grad '13-'15. Receive 50% off ticket prices for season seats. Bench $122.50, Preferred $145, Premium $172.50, Elite $200. (Excludes Family Plan & GA seating. Limit 4)
Faculty/Staff - Receive 20% off ticket prices for season seats, plus WAF donation match and/or $50 per seat WAF requirement waived. Bench $195, Preferred $232.50, Premium $275, Elite $320. (Excludes Family Plan & GA seating. Limit 4)
Rose Bowl season parking is a benefit offered exclusively to UCLA football season ticket holders and costs $174 for the 2015 season. Lots are allocated based on Wooden Athletic Fund donor priority and are outlined here.
For Season Parking Pass questions, call the UCLA Central Ticket Office at 310-UCLA-WIN (310-825-2946).
Parsons' Season Parking in Pasadena - $78
A season parking pass for the Parsons' parking lot in Pasadena is available to all season ticket holders for $78 and includes free roundtrip shuttle to and from the Rose Bowl on game day, beginning 3 hours prior to kick-off.
Single-Game Parking Options
- Parking in Lot H and Brookside Park and Golf Course will be sold by the City of Pasadena on gameday only, for $25 per car.
- Parking is also available at the Parsons' lot in Pasadena for $15 on game day and features a free roundtrip shuttle to and from the Rose Bowl, beginning 3 hours prior to kick-off.
- RV parking is located on West Drive for $50 and opens 6 hours prior to kick-off.
Being a UCLA Football Season Ticket Holder has it's advantages! Check out all of the great benefits you receive with your season tickets.
|Best Seat Locations
UCLA Football Season Ticket Holders receive the best seat locations! Enough said.
|Seat Upgrades
Change/improve your seat location each year at UCLA's "Select-a-Seat".
|Season Parking
Season parking at the Rose Bowl is available for all season ticket holders (while supplies last).
|Resell & Forward Tickets
Use UCLA's Account Manager to manage your season tickets.
|Season Ticket Trade-In
Turn in your unused tickets from past games for extra seats to future games! Excludes USC and Oregon.
|UCLA Ticket Priority
Get exclusive presales and discounts and free ticket offers to UCLA Athletics events.
|UCLA Merchandise
Receive discounts to the UCLA Store.
|Postseason Tickets
Priority seating to UCLA Bowl games
|Collector's Pin
Receive an annual collector's pin for each season ticket. A 29 year tradition!
|Bruin Blue
Free subscription to Bruin Blue, the official magazine of UCLA Athletics.
Bruin Season Seat Cushions
Lease today for just $35 for the entire season! (sales tax included).
- Enjoy the comfort of home at the stadium by reserving a seat cushion with your season tickets
- Cushions are permanently attached to your seat location and are maintained throughout the season.
- With six home games scheduled, enjoy the gridiron action in style for less than $7 per game.
UCLA Football Season Ticket Transfer Policy
In maintaining the integrity of the UCLA Football seating program, season tickets for football can be transferred with a one-time gift to UCLA Athletics of $25,000. For gifting details or additional information regarding the UCLA Football Season Ticket Transfer Policy, please contact the UCLA Athletics Development Office at 310-206-3302.
2015 UCLA Football Mini Plans
Catch the biggest match-ups of the season with a 2015 UCLA Football mini plan! Order now and receive:
|Sept. 05
|UCLA vs. Virginia (Home Opener)
|Oct. 03
|UCLA vs. ASU (Pac-12 Opener)
|Oct. 31
|UCLA vs. Colorado (Homecoming)
Choose three or more games and receive 5% off the total single-game price:
Three Games: $138
Four Games: $184
Five Games: $230
Or call (310) 206-5991
Guest Bus Passes - $20
2015 UCLA Football Single-Game Tickets
Tickets to all SIX UCLA Football home games are now on sale!
|UCLA vs. Virginia
|UCLA vs. BYU
|UCLA vs. Arizona State
|UCLA vs. Cal
|UCLA vs. Colorado
|UCLA vs. Washington State
2015 UCLA Football Single-Game Ticket Pricing Preferred / Premium / Elite: $61 & Up
Bench: $49
General Admission Adult: $33
General Admission Youth: $5*
Prices are subject to change. Listed price does not include fees.
*12 years of age & under. Youth tickets sold on gameday at the Rose Bowl only.
Or call 310-UCLA-WIN (310-825-2946)
For the best seat locations and ticket prices, purchase your 2015 season tickets now.
Bruin Bash
Show your team spirit at the ultimate pre-game party for UCLA alumni and fans. Kick-start game day, Bruin style, with friends, food and entertainment. Be a part of the excitement. Don't miss out on the fun!
To sign up or more information click here.
UCLA Football Group Tickets
Receive 20% off reserved seats for every game and great benefits when you order 20 or more group tickets.
Call: 310-206-5991
Email: firstname.lastname@example.com
Opponent Fans
Group seating through the UCLA ticket office is offered specifically for UCLA fan groups. Fan groups for opposing teams should contact their home ticket office to directly purchase tickets.
Blue Moon Club Hospitality
- Ticket in Elite/ Premium/ Preferred Area
- Access to Blue Moon Club in Terry Donahue Pavilion
- Starting at $84 per person
- Ticket in Bench Area (upgrades available while supplies last)
- Access to Ralph's Ultimate Tailgate in Lot H Fan Zone
- Food and Three Drink Coupons per person included
- Starting at $65 per person
Corporate Hospitality
- Ticket in Bench Area (upgrades available while supplies last)
- Private Hospitality Space in Lot H Fan Zone
- Open Bar & Catered Food included
- Rentals for Tables, Chairs, etc. included
- Starting at $250 per person
Call 310-206-5991 or email email@example.com for more information or to reserve a package today.
The Rose Bowl offers a premium lounge for fans called the Blue Moon Club. Located in the Terry Donahue Pavilion with a view of the west concourse and arroyo, access to the area is granted by membership only. This exclusive club boasts numerous amenities and perks. Plus, it's just steps away from your season seats!
Food & Drink: The Blue Moon Club offers premium concessions for purchase and a full cash bar. It's one of the only spots in the stadium to grab a beer, wine or cocktail.
Extended Access: Entry into the Blue Moon Club begins two hours prior to kickoff. Post-game, the Blue Moon Club stays open one hour after the last play and serves alcohol for approximately 30 minutes after the game.
Amenities: The shaded lounge features upscale restrooms available only to club members, seating areas and a multitude of flat screen televisions.
Easy Access: The Blue Moon Club is accessible from the west concourse via escalators and elevators, and through the seating bowl itself. Entrances are located at the top row in sections 15 - 22.
Season Pass: Season-long access to the Blue Moon Club is $300 per person.
UCLA Football Away Games
Sit in the UCLA section for Bruin road games! UCLA Football Season Ticket holders were offered the exclusive opportunity of ordering away game tickets prior to June 1. All UCLA fans may now order away game tickets, subject to availability, by calling 310-UCLA-WIN (825-2946) or logging into their Account Manager.
Purchase 2015 UCLA Football Away Game Tickets:
@ UNLV – September 12*
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Everyone is mentioning Argon rich environment. Can spray welding be done with 100% Argon?
Results 31 to 38 of 38
Thread: spray welding
07-20-2007, 12:28 AM #31I'm not late...
I'm just on Hawaiian Time
I want to try this but ,the local welding supplies store is closed darn ! Like Bert said can you do it with 100% Argon?
Inferno Forge
07-24-2007, 01:05 PM #33Member
- Join Date
- Apr 2007
- Location
- Nothern Colorado
- Posts
- 59
Sorry it takes so long for me to reply, I'm rodeoing allot right now, and I'm gone for a couple weeks at a time.
Yes, 100% Argon is fine. Sheilding gas mixtures are used for a variety of reasons. One is that an expensive gas is mixed with a ceaper gas to lower the overall cost. Another is influence of the weld puddle and arc characteristics. Helium is often added to increase penetration, oxygen as a stabalizer, CO2 because it's cheap. There are advantages and trade offs with all of them. For example, 100% Argon gives a very attractive bead, but its not as cheap as CO2, but CO2 gives allot of splatter....
07-24-2007, 03:21 PM #34Senior Member
- Join Date
- Sep 2006
- Location
- Wichita Falls, Tx
- Posts
- 177
I read through here briefly, so It may already have been covered and I missed it.
What Is the reccomended gas for a MM210 to spray arc in. 90Ar/10Co2, 93Ar/7CO2 or 98Ar/2O.
I read someone say that the BEST to spray in is the 98/2. but will the MM210 spray in the other two gasses as well? (I known other high amp welders will spray in all three, but does the mm210 have enough amps to spray in the 90/10?)
Is .030 reccomended or can I use .035?
What a good starting point for the settings (amps and wire speed).
I think that all my questions for now.
Thanks you,
07-24-2007, 06:32 PM #35Senior Member
- Join Date
- Sep 2005
- Posts
- 867
With 98/2 the 210 will spray arc an .030 or .035 solid wire. I prefer the .030 for its higher current density. Meaning at the same output power level , the .030 offers the potential to produce a deeper penetrating weld. On the 210 , the only advantage an .035 solid wire offers over an .030 in spray transfer mode is the ability to produce the weld bead quicker. For the recreational weldor, who doesn't weld everday , I personally don't look upon this as an advantage, because it gives you less time to read the weld puddle.
With 98/2 voltage tap #7 and the wire speed around 75- 80 with an .030 will get you started. The amount of wire speed need is going to depend on your input voltage. I'd start out at 80 and reduce if needed. With 98/2 there is also some spray arc range available with Tap #6 too. Sorry can't remember a wire speed range to get you started with on this tap though.
Bareback Jack,
THANKS DUDE! I'll try it this weekend !!!!I'm not late...
I'm just on Hawaiian Time
07-25-2007, 12:17 AM #37Senior Member
- Join Date
- Sep 2006
- Location
- Wichita Falls, Tx
- Posts
- 177
just to let everyone know, I tried spray with 100% argon and it did NOT work. It would start ont in spray the after an inch or so it would go back to globular trans. There was lots of undercut and the weld bead was high and narrow. (I tried to weave the gun for tie in but it would go into glob trans when I did that) Anyways do you move the gun only in a straint line or shoul I weave it for tie in???
Also would the lincoln Pm215 (has 250 amps) be any different for the gas usage?
ace, I'm just wondering...did you try it with more power so that 1" of spray would last longer?
thanks for your testing and input! Let mem know what happens!!!
Oh, and what was your settings at?
bertI'm not late...
I'm just on Hawaiian Time


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Minor Bay Lodge & Outposts, a deluxe, American-Plan, fly-in fishing lodge, offers the ultimate in catch & release angling for Monster pike, lake trout, walleye and Arctic grayling in a pristine wilderness setting. Maximum only 18 guests per trip.
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Premier halibut, salmon, lingcod fishing for 30 years. Also offering: water taxi service, kayaking, remote cabin rentals, scenic cruises, hiking, clamming, flight seeing, lodging, birding and photo cruises, transportation to Kachemak Bay State Park
Dolphin safari boat is fully refurbished and ready for your exclusive charter trips. We offer fishing trips, surfing trips, scuba diving trips and many more types of trips catered to your interests upon request.
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Fishing near Gulf Shores for your family or friends on your Gulf Shores vacation. 3hr, 4hr, half day or full day fishing trips. Year round fun and fishing. Cruises available for Spring Break. Private trip for you and your group up to 6 people.
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Guided World class Atlantic Salmon Fishing on the world famous Miramichi river,the river where nearly half of all Atlantic Salmon in North America is caught each year.With choice of all inclusive or self catering packages and 11 private fishing pools
You will experience a complete full service Light Tackle charters. We will take you from Cangrejos Yacht Club to the Torrecilla Lagoon and San Jose Lagoon, two of the most exciting Lagoons in Puerto Rico. Great for all family members. No Sea-sick.
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Burkhard Loffeld is the newest member of the Genetic Identity sales team. He joined the team in April of 2010. Before joining Promega, Burkhard received his Diploma in Biology from the University of Muenster, Germany. Burkhard has worked at Promega's German branch as a Field Applications Specialist since 1999, where he provided sales support for the complete Promega product portfolio. Recently, he became more involved in supporting Genetic Identity products, especially the new PowerPlex® ESX and ESI Systems. In his new role at Promega, GmbH, Burkhard will be responsible for all Genetic Identity products in Germany and Austria. He also will represent Promega at national forensic meetings.In his spare time Burkhard enjoys music, running and spending time with family and friends.
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The city of Macon encompasses the following counties: Bibb. Macon's government is comprised of the Mayor, who is currently Robert Reichert (D).
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|Simsbury, Conn.
As a sophomore in 2009: Didn't play due to injury.
As a freshman in 2008: Started all 15 games she appeared in last season...Helped Quinnipiac to a league-low of 15 goals-against in 2008...Ranked third in the NEC in goals-against average with a 0.93...Made 56 saves with a 7-6-2 record...Tied for third in the NEC with four shutouts.
Before Quinnipiac: Played all four years at Simsbury High School in Simsbury, CT...Was voted on to the NSCAA All Region Team in her senior season...Was voted on to the CIAC All-State Soccer Team in her senior season...Also voted on to the Hartford Courant All State Soccer Team in her senior season.
Personal: Native of Simsbury, Conn...Daughter of Michele and Richard Kelley...Has three siblings: Jake, 23, Samantha, 19, and Abby, 16...Sister, Samantha, plays soccer at the University of Connecticut...Father, Richard, played soccer at Boston College. Kelley was also voted to the All-State Track Team in 2006...Physical Therapy major.


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Leaving the military and entering the civilian world can be a hard transition for many people to make. The Transition Assistance Program is one way the military helps those leaving the service.
The Defense Department's TAP program was designed to smooth the transition of military personnel and family members leaving active duty. The program has two parts. First is the Transition Assistance Management Program, a mandatory briefing for all separating Airmen. Second is TAP, a volunteer program troops are encouraged to attend. Both parts prepare Airmen for their separation.
"I recommend everyone thinking of separation go to the voluntary TAP,†said James Knuckles, 56th Force Support Squadron community readiness consultant. "The program has benefits like resume writing, interview techniques and benefits available to separated members.
"The TAMP briefing is mandated by the DOD,†Knuckles said. "It's only four hours, and it covers a lot. However, TAP is three days and breaks things down better for those separating.â€
The program helps every Airman whether they are leaving after their first enlistment or those who are finishing up 20 years.
"The program was extremely helpful toward identifying areas of concern that I had not thought of,†said Tech. Sgt. Ejimofor Farrow, 56th Logistics Readiness Squadron refueling maintenance NCO-in-charge. "Areas such as life insurance, education, Veterans Affairs benefits and career preparation after the military were covered in the program. The focus on resume writing and job search tools were extremely helpful. Also being reminded of the difference between the military environment, steeped in tradition and rank structure and the civilian sector, which is most likely centered on the bottom-line principle in an economy that is shaving the fat. This was an eye opener.â€
As the job market and economy change in the civilian world, the TAP changes as well to provide the most up to date information to service members.
"This was my second time through the TAP program before I retire, and I was amazed at how much had changed within eight months,†Farrow said. "As a career NCO, you rely heavily on past military experience and consistency of regulation. While the times are changing so rapidly, policies and procedures that applied six months ago no longer apply today. It was crucial that I attend the TAP briefing which allowed me to get clarification on the policies that affect my pending retirement and the options available to me.â€
The Airmen and Family Readiness Center conducts and holds the TAP and TAMP briefings and a new class is scheduled to begin Thursday. There are also online resources available from the Department of Labor, the VA and all the military branches that work in conjunction with the programs.
"The most significant element of the briefing, I thought, was establishing a relationship with the VA representative,†Farrow said. "She was able to walk me through many of the medical forms and documentation to track missing medical records as well as prepare for the legwork involved with filing the claim.
"This is one of the best military support programs that I have encountered in my career, and the knowledgeable staff members make it a rewarding and pleasurable experience. This outstanding program makes it easier to minimize the anxiety of transitioning from the military world into the civilian sector and offers the military member and military family significant tools to prepare for the change.â€


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A New Castle man learned Thursday night it's not a good idea to run from a police dog.Steven Roscoe, 31, was arrested on an outstanding warrant and drug charges after a foot chase that ended with him being dragged to the ground by Dover Police Department K-9 officer Spike, according to department spokesman Cpl. Mark Hoffman.
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Coins and currency are more than just things we use to make purchases. They also can be windows into the past.
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Recipe by KDCG
"Tony is my husband. He brought home a recipe he had gotten from someone at work, and it was adapted over time to this recipe--so I named it Tony's Summer Pasta. The tomatoes and cheese are marinated in oil, basil, and garlic, and then tossed with the hot linguine. The tomatoes are warmed and the cheese melts ever so slightly, creating a simple but delectable meal."
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1 (16 ounce) package
linguini pasta
roma (plum) tomatoes, chopped
1 pound
shredded mozzarella cheese
1/3 cup
chopped fresh basil
6 cloves
garlic, minced
1/2 cup
olive oil
1/2 teaspoon
garlic salt
ground black pepper to taste
I used bocconcini (little fresh mozzarella balls) instead of the shredded mozzarella and red-ripe Roma tomatoes, seeded and chopped. I skipped the garlic salt - totally unnecssary. To avoid a strong taste of raw garlic I mixed the minced garlic with a little salt, then used the flat side of my chef's knife to mash it into a paste. I added all ingredients to taste rather than measuring. A plate of this and I'm happy. Very happy.
This was dinner for us last night. I used whole wheat pasta, cut down the mozzerella cheese in half and cut the EVOO WAY down to a 1/4 c., if that. I also used fresh garlic. My boys seemed to like this but my husband thought it would have been better with a mixture of parmesan and mozzerella. Good base recipe to tweak any way you like to make it suit your family.
110 Ratings
Simple, delicious - and not just for summer! Although, I'll definitely remember this for summer when garden ripe tomatoes are in season. I subbed penne pasta for the linguini and opted to use fresh mozzarella that I sliced and chopped vs. shredded. I also used regular salt vs. garlic salt. This is a great side dish for any meal or you could make this a meal in and of itself it's so good. Next time I might add a splash of balsamic vinegar or a bit of crushed red pepper flakes for a little pizzaz!
I was really excited about this recipe and followed it exactly except I added 1/4 lb of prosciutto. However the garlic really overpowered the taste from the tomatoes and the basil. We love garlic, but it is very powerful raw. I would decrease the amount of garlic if I were to make it again.
This recipe is fantastic! I served Tony's Summer Pasta for a family dinner party and everyone wanted me to email the recipe to them. Someone even suggested you could try feta cheese instead of the mozzarella.
Basically a good recipe idea, but I would make
the following changes:
double the tomatoes & basil
4 cloves (instead of 6) of garlic
cut the cheese in half
very good and full of flavor. in order to cut down on the calories, i left the cheese out of the recipe completely and added a few more veggies to the marinade. i put some grated parmesan on the table when i served it so people could add a little parmesan to the top if they wished, but no one really missed it. it was a huge hit. i was able to serve this again cold as a nice summer pasta salad using bite-sized pasta instead of the pasta strands. fresh lemon is great in this dish as well!
Try this with brie cheese instead of the mozzarella. Cut rind off first, and tear or cut into small chunks. It's the BEST! I've had this recipe for 20 years, and make it every summer with fresh tomatoes and basil from my garden. The brie is a must-try!
* Percent Daily Values are based on a 2,000 calorie diet.
Tony's Summer Pasta
Serving Size: 1/6 of a recipe
Servings Per Recipe: 6
Amount Per Serving
Calories: 635
Calories from Fat: 287
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text: Info Search results 5 items matching your search terms. Filter the results. Item type Select All/None Collection Page Event File Folder Form Folder Image Link News Item Collection Person New items since Yesterday Last week Last month Ever Sort by relevance · date (newest first) · alphabetically Adult Hearing Aids and BAHA Located in Adult Services / Adult Audiology CASTLE CASTLE has been helping deaf and hard of hearing children learn to listen and talk since 1993. We provide services to children and families in North Carolina and across the country. We train professionals across the world to help children with hearing loss learn to speak. CASTLE is a school, a training program, a therapy center, and family support program. Hearing services are provided down the hall at CCCDP. Located in Pediatric Services Hear-N-Now Registration Registration for the 21st Annual CCCDP and NC AG Bell "Hear-N-Now Conference" November 6th and 7th, 2014 UNC researchers to take part in multi-center, $8.9 million study of childhood hearing loss University of North Carolina at Chapel Hill researchers will play a key role in a five-year, $8.9 million study aimed at understanding the impact hearing loss can have on children's ability to communicate, succeed in school, and have good social and psychological development. Located in News Welcome


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Illegal Immigration & Interior Enforcement
Illegal Immigration & Interior Enforcement
Recent Activity
The Migration Policy Institute celebrated its first decade as the authoritative, unimpeachable resource on immigration and immigrant integration analysis and policy design in the United States and internationally.
MPI Senior Fellow Doris Meissner discusses U.S. immigration policy during the Fireside Forum on Foreign Policy at the University of Wisconsin-Milwaukee. The other participants are Christine Neumann-Ortiz, Executive Director of Voces de la Frontera and Douglas Savage, Assistant Director of the Institute of World Affairs, University of Wisconsin-Milwaukee.
The conference offered law and policy analysis and discussion on cutting-edge immigration issues. Featured panelists included high-ranking government officials, academics, advocates, and other immigration experts.
Public Policy Institute of California (PPIC) research fellows Magnus Lofstrom and Sarah Bohn, and UC Berkeley professor of public policy Steven Raphael are issuing a new report that examines whether LAWA achieved its primary aims: reducing the unauthorized population, deterring their employment opportunities and improving employment outcomes of competing authorized workers. Their analysis also investigates whether Arizona's legislation induced a shift away from formal employment.
This discussion focuses on the MPI report, "Executive Action on Immigration: Six Ways to Make the System Work Better," which outlines administrative actions that can be implemented to improve the immigration system.
The conference offers thoughtful, evidence-based law and policy analysis and discussion of cutting-edge immigration issues.
Join MPI as U.S. Citizenship and Immigration Services Director Alejandro Mayorkas discusses the application process and policies that the agency has announced to implement the administration's deferred action program.
The Migration Policy Institute celebrated its first decade as the authoritative, unimpeachable resource on immigration and immigrant integration analysis and policy design in the United States and internationally.
Through the Prism of National Security: Major Immigration Policy and Program Changes in the Decade since 9/11
With the ten year anniversary of the September 11, 2001 attacks approaching, the Migration Policy Institute held a conference call to discuss the most significant changes that have occurred in the immigration arena in the decade since the attacks.
This Migration Policy Institute webinar discusses labor enforcement laws during the Clinton, Bush and Obama administrations and chronicles gaps in labor protection.


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"Inside the Clubhouse: The Players' Perspective" is the story of 2011 Giants baseball from those who know it best? the players themselves. SFG Productions, the Emmy-winning in-house production company of the San Francisco Giants, captures the best moments from the Giants' exclusive Breakfast with Champions event held August 6, and goes behind the scenes with Brian Wilson as he gives away 1,000 baseball gloves to well-deserving Jr. Giants, whom he also treated to the July 7 game. As manager Bruce Bochy attests, these San Francisco Giants are truly "Characters with Character." From Ryan Vogelsong's lessons on cap-tipping technique to Javier Lopez's tales from the bullpen, "The Players' Perspective" showcases the best of the San Francisco Giants, on and off the field.
What's the weirdest autograph request Buster Posey has ever received? What snack does Brian Wilson crave after games? How old do you have to be to throw a curve ball? What motivates Pablo Sandoval? And how does Cody Ross really feel about Philadelphia fans? The San Francisco Giants address these questions and more when "Inside the Clubhouse: The Players' Perspective" premieres Sunday, September 4 at 12pm on Comcast SportsNet Bay Area.


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1760 North Lincoln Street
Dixon, CA 95620
Now Serving You in Two Convenient Locations
Welcome to the used car inventory page of Ron DuPratt Ford. You'll see from our selection above that we have a large Dixon CA used car inventory, and we know that we have a used car, truck, mini-van or SUV for you! Take a look, and then call us with any questions; or stop by 1760 North Lincoln Street for a test drive.
Visit Ron DuPratt Ford for all your Dixon CA Ford needs. From used cars to new 2012 - 2013 Ford cars, and from car repairs to Ford parts, we have California covered! Come talk to the automotive professionals at our Dixon CA Ford dealership and we'll be happy to assist you.We have been in business here for over 50 years and we wont be beat by any local dealer to earn you business.


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axle-mounted base bag
Be ready to roll!
This drop & go storage bag fits snugly over the axle of your vibe or smart stroller, or securely into the parcel tray of any other phil&teds. Secure zip pockets and a carry handle let you grab, drop & go!
Actualmente no está disponible en esta región.
base bag ™ storage is HOT! because:
- axle-mounted, a perfect fit on your vibe or smart stroller
- fits securely into the parcel tray of any other phil&teds stroller, plus many other brands
- zips closed - no chance of bits & pieces bouncing out on the go!
- durable, wipe clean polyester fabric
- complete with handle for swift pick up & go
- compact & close fitting - will not disrupt your gait
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Home > Sports Memorabilia > More Sports > Golfing Collectibles > Pebble Beach 7th Hole Framed Golf Print
Pebble Beach 7th Hole Framed Golf Print
Handsomely framed print of one of golf's most famous holes.
Captures the beauty of Hole 7 on the Pebble Beach Golf Course in this beautifully framed print. Features green and burgundy double matting and makes a great gift. This framed print is perfect for the home or office.
24" L x 30" W x 2" H;
17" L x 19" W x 2" H
Pebble Beach 7th Hole Framed Golf Print
Reviewed by 1 customer
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Comments about Pebble Beach 7th Hole Framed Golf Print:
My husband dearly loved this framed golf picture for his office. We just moved into a new home, and I decorated his office in everything golf. Needed that one special thing on a large wall, and found this picture via internet. Framed just perfectly, and we are both very pleased. Thank you so much for the quick turn a round, received it packed nicely and secure. Looks great in the office. Thank you so much---Judy Mensik
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