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Select shots from the Fire's draw with Real Salt Lake
The Fire head west to Utah on Saturday night after dropping two games against the Union both at home and in Philadelphia. The team is without a goal in three games and face a daunting task against \ in-form Real Salt Lake (7:30pm CT on My50). RSL swept Chivas aside last weekend, cruising to a 4-1 victory. Here are some things to look out for from a tactical perspective.
The return of Soumare – more changes for the Fire back line?
On Thursday, the Fire acquired former player Bakary Soumare from the Union. Soumare will provide some much-needed depth at the center back position with the long-term future of Arne Friedrich in doubt.
Soumare's return comes at a good time for the Fire who are without a number of players due to injury and suspension. With Wells Thompson suspended, Frank Klopas could choose to move Jalil Anibaba to the right side of defense and slot Soumare in alongside Austin Berry.
Athletico Coaching Corner: Real Salt Lake
The injury to Friedrich has meant a longer than expected spell in the middle for Anibaba and in his absence, the right-back position has changed hands on numerous occasions this season.
READ: Soumare ready to start vs. RSL
Though right-back isn't Anibaba's natural position, the defender has played there for a lot of his Fire career and is comfortable in it.
Against a team like Salt Lake who are known for their aerial prowess, another big body on the back line like Soumare's in addition to Anibaba and Berry would be very useful.
Getting some consistency in defense is very important from here on out and a starting four of Anibaba, Soumare, Berry and Segares would appear to have the right blend of youth and experience.
The Fire's attack – pace vs possession
In both games vs. the Union, coach Frank Klopas started Patrick Nyarko up front with Chris Rolfe in an attempt to bring some speed to the forward line.
Against Salt Lake however, a more logical tactic would be to use a bigger body who can hold the ball up and can be used as an outlet to relieve pressure.
Sherjill MacDonald is fit again after an illness and could start up front as the target forward. This would allow the Fire to move Patrick Nyarko back to the wing, possibly in place of Joel Lindpere who has logged a lot of minutes this season.
READ: Nyarko keeps ticking despite reckless tackles
Though Nyarko's creativity and pace in the attack worked well against Philly, his desire to track back and help the defense could prove very important, especially against a team with a wealth of attacking options who are so offensive minded at home.
The problem with starting MacDonald up front is that he can oftentimes be left devoid of support and it will be up to players like Rolfe, and Duka to get forward and support the Dutchman.
If he does get the nod, the battle between him and the RSL center backs such as Kwame Watson-Siriboe will be very interesting to watch.
RSL's midfielders – tracking runs into the box
RSL plays with a diamond midfield system with Kyle Beckerman anchoring and Javier Morales playing at the top of the diamond. Morales is the team's playmaker and oftentimes acts as a third striker, getting forward to support the front two.
Duka, Larentowicz preview Real Salt Lake
Though Morales is an obvious target, players like Ned Grabavoy and Luis Gil who play on the flanks cut inside on a regular basis, making runs into the middle when joining in the attack.
With this many players getting forward, the Fire must be aware not only of who is marking who but must prevent RSL from getting second ball opportunities.
Similar to the Fire for most of the season, RSL likes to play with at least one mobile striker while the other tends to play higher up the field.
The mobile striker, oftentimes Joao Plata, drifts into wide positions, usually on the right hand side. His moves out wide open up more space for players like Morales, Grabavoy and Gil to move into.
Plata has four assists this season and is a good crosser of the ball. His crossing ability was displayed earlier this month against Vancouver where he put in a perfect ball Gil to head home the winner.
Staying tight with runners from midfield and not allowing second ball opportunities will improve the Fire's chances of getting a result on the road dramatically.
Prediction: Rio Tinto Stadium is one of the hardest places in MLS to get a result in. The Fire has had no luck this season and perhaps Saturday night will be the night. 1-1 with Chris Rolfe scoring the Fire's goal from the penalty spot.
Stephen Piggott is a contributor to Chicago-Fire.com. Follow him on Twitter @Irish_Steve.
UPDATE - Segares clarified his comments following training on Wednesday.
"Its always an honor to be called up to the national team and represent your country. Regretfully at this moment the injuries the team is suffering along with the Open Cup game and league game against Real Salt Lake prevents the team from allowing me to go with the national team. If it was my decision, I would love to go with the national team but regretfully its just not the right moment. My door is always open for a national team call-up."
Last week, Chicago Fire defender Gonzalo Segares was summoned by Costa Rica for next week's international friendly against Canada in Edmonton.
With Segares having mostly gone unselected by the Ticos the last few years, the call-up was a welcome one. With the Fire experiencing a high amount of injuries, Orrin Schwarz reports the Fire left back turned it down in order to stick with the Men in Red through Saturday's game at Real Salt Lake and Wednesday's U.S. Open Cup match at Charlotte Eagles.
"We have important games coming up, and it's not a FIFA date, so I cannot leave my team right now," Segares said. "We need to stay together. It's not the right time for me to go."
Should the Chicago Fire come away victorious over the Charlotte Eagles in the third round of the Lamar Hunt U.S. Open Cup next Wednesday, the team is guaranteed to host its fourth round match against either the Columbus Crew or Dayton Dutch Lions on Wednesday, June 12 at Toyota Park.
I'll have a full play-by-play of the draw later today on Chicago-Fire but until then, see below all fourth round U.S. Open Cup matchups:
#1 – New England Revolution/Rochester Rhinos hosts Reading United AC/New York Red Bulls winner
#2 – D.C. United/Richmond Kickers winner hosts Ocean City Nor'easters/Philadelphia Union winner
#3 – Chicago Fire hosts Dayton Dutch Lions/Columbus Crew winner
-or- Columbus Crew host Charlotte Eagles
-or- Dayton Dutch Lions host Charlotte Eagles
#4 – Sporting KC host Colorado Rapids/Orlando City winner
-or- Colorado Rapids/Orlando City hosts Des Moines Menace
#5 – FC Dallas hosts FC Tucson/Houston Dynamo winner
-or- Houston Dynamo hosts Ft. Lauderdale Strikers
-or- Ft. Lauderdale Strikers vs. FC Tucson (Host TBD)
#6 – LA Galaxy/Carolina Railhawks winner hosts Chivas USA/LA Blues winner
#7 – Atlanta Silverbacks/Real Salt Lake winner hosts San Jose Earthquakes/Charleston Battery winner
#8 – Seattle Sounders will host Wilmington Hammerheads/Portland Timbers winner
-or- Portland Timbers will host Tampa Bay Rowdies
-or- Tampa Bay Rowdies vs. Wilmington Hammerheads (Host TBD)
"There was thunder in our air; nature, as we embodied it, became overcast -- for we had not yet found the way. The formula of our happiness: a Yes, a No, a straight line, a goal..." - Friedrich Nietzsche
Be wary of people who throw around Nietzsche quotes. His writing is so punchy that it's easy to find good quotes inside of paragraphs about almost anything. Notice: I can take a line from a book Nietzsche wrote called The Anti-Christ and make it about soccer.
But if I can take a quote about anything out of context and pass it off as fair, it's a quote about goals, right? Goals, too, have a way themselves of coming out of context. Ask the Fire about it recently.
Where do goals come from? In no sport is scoring as mystifying, and, as a result, as satisfying. Goals are rare enough to be special, but common enough to drive the sport completely, and their origin remains a mystery. What fickle furnace forges them? They come from the heavens, where so many players give credit in their celebrations, and from an entire team's psychic connections. They come from perfect timing, impeccable technique, and acts of brilliance. They also come from deflections, bad refereeing, and those mis-kicked crosses that loop into the back post.
Where do goals come from? Don't ask the Fire right now. A team that hasn't always had problems scoring, the Fire find themselves in goal purgatory. For whatever reason, goals hate the Fire right now. They played well enough to at least earn a point out of Saturday's game, and absolutely dominated the first matchup against Philly last week -- but the Fire couldn't score. The drought has become confounding, almost like a natural disaster, something to marvel at in awe and horror.
It hurts even more to play against Jack McInerney twice in a row. McInerney has more goals this season than the entire Fire squad. He's so hot that goals are showing up in his bed at night. He's waking up next to goals he doesn't remember meeting.
Okay, okay. The point is that goals are not to be trusted. They're misleading. Goal stats rarely tell the story of a game, especially when teams control a game without scoring, like the Fire did two weeks ago and in periods on Saturday.
After a few unlucky breaks and a scuffed chance or two, it can feel like everything is conspired against you. The ref hates you, the ball and the vagaries of its deflections hate you, the goals themselves, they look so small now, even with their giant looming posts and their soft, welcoming nets -- don't trust them, they definitely hate you. Meanwhile, set plays executed perfectly in training don't come off. You start to over-think simple five yard passes. Your shoelaces untie themselves. It's excruciating. You can work, you can run, you can do everything you can, but nothing works.
Where do goals come from? Can Klopas and Pause go on some sort of vision quest to find some? The Fire are getting shots (22 over the last two matches vs. Philly), managing games, and getting chances, but the payoff is late.
Well, maybe there's more in the Nietzsche about goal-droughts that I thought. Much of The Anti-Christ is in response to Arthur Schopenhauer's cycle of desire and dissatisfaction, the cycle that defines
goals humanity. But like the Fire, Nietzsche is concerned with how we overcome our contemporary (~1880s) problems. He says that despite the fact that we once found happiness, we lost it. "We grew dismal; they called us fatalists."
But once "there was thunder in our air" and surely we'll recover it. "A Yes, a No, a straight line, a goal..."
Ben Schuman-Stoler is a contributor to Chicago-Fire.com. Follow him on Twitter @bsto.
Select shots from the Chicago Fire Rec Soccer league Final at Toyota Park on Sunday
The Fire's summer friendly opponents and Team for the World alliance partners Club America took a further step to their first domestic crown since 2005, defeating Monterrey 2-1 (and 4-3 on aggregate) in leg two of the Liga MX semifinals Saturday night at the Estadio Azteca.
In leg one last Wednesday, Ecuadorian international Christian Benitez continued his exploits from the weekend's 2-1 victory over Pumas UNAM, scoring both goals in the team's 2-2 draw at Monterrey.
That result set the stage for Saturday's match, which saw America take the advantage just after the hour mark. In the 62nd minute, Aguilas midfielder Ruben Sambueza was brought down on the right side of the area by Monterrey defender Leobardo Lopez, forcing referee Jorge Antonio Perez to point to the spot.
A minute later, Aguilas striker Raul Jimenez placed his effort past Jonathan Orozco into the lower left netting to give America a 1-0 lead and 3-2 aggregate advantage.
The home side pretty much iced the series 19 minutes later after a Monterrey giveaway at midfield. Jimenez and Benitez quickly broke out in a give and go before the Ecuadorian was slipped into the box and finished past Ibarra for the 2-0 lead and 4-2 advantage.
The goal was Benitez' fifth in four playoff matches.
Los Rayados pushed late and pulled one back deep into second half stoppage time. Substitute Guillermo Madrigal settled in the box, turned and forced a diving stop out of Moises Munoz before striker Aldo de Nigris finished from close range.
The goal to the aggregate score to 4-3 but the referee blew the whistle soon after, advancing America to the Liga MX Clasura finals against Cruz Azul.
Leg one of the finals will be played at Estadio Azul in Mexico City on Thursday with the return leg at Estadio Azteca on Sunday.
The Chicago Fire take on Club America in an international friendly on Wednesday, July 10 at Toyota Park.
Atletico de Madrid takes Copa del Rey title
Atletico Madrid over came a 25-match winless run against city rivals Real Madrid and a 1-0 deficit to win the Copa del Rey 2-1 in extra time at the Santiago Bernabeu on Friday night.
Real Madrid took the early lead when Cristiano Ronaldo rose above the rest to head home a corner kick in the 14th minute.
Atletico would respond 21 minutes later when Radamel Falcao did yeoman's work at midfield before springing Diego Costa between two Madrid defenders. The Brazilian fired from the top of the penalty area and beating 'keeper Diego Lopez inside the left post.
You could see Real Madrid begin to come undone in the 75th minute when manager Jose Mourinho was sent off for leaving the technical area to argue a play.
Fifteen minutes later, Cristiano Ronaldo kicked Atletico defender Juanfran but the offense went unnoticed and the teams went to extra time level at 1-1.
Atletico would find the game-winner off a corner. Koke's take from the right was headed straight back to him but he whipped in another ball which found the head of Brazilian defender MIranda to beat Diego Lopez inside the near post.
The wheels came off for Real Madrid when Ronaldo was sent off following a scuffle at midfield in the 115th minute.
Shortly thereafter the referee blew the whistle, confirming Atletico's 2-1 triumph. The victory gave the club its 10th Copa del Rey title and first since 1996.
Select shots from the Fire's match with the Union
Credit: USA Today Sports Images
The Fire head to Philadelphia on Saturday night to take on the Union for the second time in seven days (Coverage begins 6pm CT on My50). The Men in Red dropped a heart breaker 1-0 at home last week despite creating a number of chances. Philly played LA at home at midweek and lost 4-1 after a second half defensive collapse. Here are some things to look out for from a tactical perspective.
Rolfe and Nyarko up top – repeat the first half of last week
In the first half against the Union at Toyota Park last week Patrick Nyarko terrorized the Philly defense, running at them with pace and creating a number of opportunities.
Rolfe and Nyarko combined well and were very close to each other on the pitch. The movement of the pair dragged the Philly center backs out leaving massive pockets of space in behind for the Fire to exploit.
WATCH: Nyarko, Berry Preview Philly
This happened on a number of occasions and NBC analyst Kyle Martino pointed it out over and over again on the broadcast.
In the second half however, the duo were too far apart and then Rolfe was moved out wide later. It goes without saying they'll need to play closer together on Saturday.
When Rolfe and Nyarko force the center backs out of position, players like Dilly Duka and Joel Lindpere need to do a better job of tucking in and taking advantage of that space, something the team didn't do enough of last week.
Philly were unable to deal with the pace of Nyarko last week and the Fire should look to get the ball to the Ghanaian as much as possible again Saturday.
Kleberson – keeping an eye on the "unknown" Brazilian
In Philly's game against the Galaxy Wednesday night the Union gave a first start to Kleberson in midfield. In the first half, LA was unable to deal with his movement and defense-splitting passes.
Kleberson got forward constantly to help out Jack McInerney and Sebastian Le Toux in the attack and was allowed two or three shots from just outside the box. He also dropped deep and found the strikers with some brilliant through balls.
In the second half, LA was a lot tighter on the Brazilian and he was less effective. Though he may not be fit enough to play the entire 90 minutes against the Fire, I would expect him to start after his excellent full debut against LA.
Logan Pause and either Daniel Paladini or Jeff Larentowicz must track Kleberson's runs and be wary of balls played in behind. Logan did a fantastic job of cutting out through balls in last weekend's match and the Fire will need a similar effort from the captain on Saturday, especially if Kleberson gets the start.
Focusing on Philly's left side – opportunities for Duka and Thompson
I spoke last week about the weaknesses of Philly defender Raymon Gaddis who filled in on the right for the suspended Sheanon Williams last weekend. Against LA on Wednesday night, Gaddis returned to left back and was again caught too far forward on a number of occasions.
On the one hand, Gaddis and Keon Daniel combine very well in the attack and are certainly a threat but both players fail to track back defensively when the Union turns the ball over.
On a number of occasions on Wednesday night Bakary Soumare had to come over to almost the left wing to cover for Gaddis who was nowhere to be found. LA took full advantage of this, attacking down Gaddis' side for most of the match.
Against Philly last weekend, right back Wells Thompson looked somewhat reluctant to come forward despite the fact that Dilly Duka was constantly coming inside and leaving space for Wells to run into. Both Thompson and Duka should be looking to attack Gaddis at every opportunity on Saturday night.
Prediction: 3-1 Fire with goals from Nyarko, Rolfe and Lindpere
Stephen Piggott is a contributor to Chicago-Fire.com. Follow him on Twitter @Irish_Steve.
Those that paid attention to the Twitter-verse during Tuesday night's U.S. Open Cup first round might have seen the name Duka being bandied about in Icon FC's 4-1 defeat of Brooklyn Italians.
No, Fire midfielder Dilly Duka isn't moonlighting out east.
The Duka in question was actually his older brother Argjent who tallied a hat trick in the New Jersey-based side's first round Open Cup win. The 27-year-old Duka scored in the 12th, 51st and 76th minute to push Icon past the NPSL side and into a second round matchup with the Richmond Kickers next Tuesday.
READ: Icon FC vs. Brooklyn Italians Match Report
Back in Chicago, Dilly forewent Twitter updates and found his own way to see the match.
"I actually watched the game through Facetime on my iPhone," he joked. "I had my sister post up in the stands and kind of record it while I watched. I didn't get a TV view of everything but I saw most of the plays."
The younger Duka isn't surprised by his brother or Icon's success either.
"He's quick, he's technical and he obviously knows how to finish. It's a good team and it's a lot of guys I play with in the offseason so their chemistry is obviously better than some of the PDL or NPSL sides now.
"I'm excited for them. I think they can make it a little further."
Duka also reacted to the news of the Fire's potential third round opponents: the Charlotte Eagles or Seattle Sounders U-23s.
"These teams are going to be excited to play against us. They're definitely going to change their formation and go more defensive. We have to go in focused and take it seriously.
READ: Fire to Enter in Third Round of USOC
"I was in Columbus the past three years – the first year we took it seriously and did okay but the second and third year we lost in our first game. Whoever we play, it'll be the biggest game of the year for them."
While Duka is a former Crew player, he knows well his current team's history in the U.S. Open Cup.
"You come here and you know the Open Cup is important. I'm aware of that and I'm sure we're going to try and live up to our name and history in the competition."


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Finished a game and got 2 sacks from Misi, one from Odrick and one from Starks. Nolan Carroll and Sean Smith both had INT's. Both Ronnie and Ricky running the ball quite a bit. Marshall is a beast. I love the game so far.
Although, Misi with long hair is annoying haha.

subset: commoncrawl


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text:
MalaBeads wrote:Another translation offers this:
If you can see through this, your Zen training is complete.
Present a sword to a swordsman;
Don't offer a poem unless you meet a poet.
When speaking say one third of it;
Don't give the whole thing at once.
LastLegend wrote:Yes sir. Now can I have the certification.
Zen masters in India said that mindlessness is Buddha. A Zen master of China said that mind itself is Buddha. He was not saying that the mind that jumps from one thing to another and the intellect that gallops off in any direction are what Buddha is.
Students in recent times often misunderstand this point. Some say, "Once you return to it, mind itself is Buddha, and there is not another life." If you understand in this way, you are the same as nihilistic heretics.
"Mind is Buddha" -
what religious teaching is this?
If you want to stop a child's crying,
give it a clout.


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Nashville up-and-comers Coldwater Jane, The Cordovas, Josh Hoge, Dustin Lynch and Eric Paslay are set to play at 5:30 p.m. Wed., Jan. 31 at Tin Roof, 1516 Demonbreun, as part of the eighth annual Music in the City concert.
The event is hosted by CMT, and a portion of the proceeds benefit Keep Music in Music City, a charity that provides musical instruments to local schools.
"I've known Eric Paslay and Josh Hoge for years, so it will be cool to take the stage with those guys," says Lynch, a Tullahoma native now signed to Broken Bow Records.
The charity is one Lynch supports, because he says his school's music program taught him a set of skills that he utilizes every day in the country music industry.
"It gave me confidence that I'm not a complete idiot on stage," Lynch says. "I did the whole show choir thing growing up. It was kind of an icebreaker gig for me. Being comfortable on stage really helped me get my foot in the door."
Lynch's debut single, "Cowboys and Angels," co-written with Josh Leo and Tim Nichols, is at country radio and available for sale on iTunes.
Tickets to see Lynch and the other artists are $20 in advance or $25 at the door. For tickets or more information, visit www.nashvillelifestyles.com.


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Go Inn Hong Kong
Flat C, 16/F, Continetal Mansion, 294 King's Rd., Fortress Hill, Hong Kong, Hong Kong, Distance from city centre: 14.8km
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- 29 Sep 2012 JIn-ah Germany


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Seca 354 Lena Baby Scale
Electronic baby scale with fine graduation, also usable as flat scales for children.
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Seca 354 Lena Baby Scale
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Faviana Style 9336
Strapless Sequin with Tulle skirt More >
This plus size mermaid style red carpet dress features a sweet heart neckline and a full sequins body that meets a flirty tulle skirt! The multiple layers of tulle give the illusion of your legs showing without them being fully exposed!
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Bedford Electric Fireplace Heater
Bring style to any room with this vent-free, easy to move heating unit.
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Bedford Electric Fireplace Heater
Bedford Electric Fireplace Heater
Our innovative products will provide you and your family with years of enjoyment. And with the Brookstone Protection Plus service and replacement plans, you'll have the added peace of mind knowing that if the unexpected does happen, we will simply repair or replace your covered product. Our enhanced coverage goes above and beyond the manufacturer's warranty, saving you time and money.
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David Bazan (born January 1976) is an indie rock singer-songwriter from Seattle, Washington. Bazan (pronounced "buh-zahn") was the lead singer and creative force behind the now-defunct band Pedro the Lion and was the lead singer of Headphones, a band he formed out of his interest in synthesizers. In early 2006, he began performing and recording under his own name.
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Marshall has the tools of a true #1, but he just has mental/consistency/hands issues.
Wallace will always be stuck in what he can do.
Pittsburgh played it how it should be played. Draft a guy like Wallace, he over-exceeds his draft position, take advantage of his rookie contact, and then let him walk to another chump team that will overpay for him.
Meanwhile Pitt has already drafted his replacement and they won't miss Wallace.
And then we will be stuck with an ''at best #2'' WR for damn near 60 mil or whatever the crazy rumor is.
Signing Wallace or Jennings will drive us further down the path of mediocrity.


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Creston Airport Commision, 6 p.m. Monday, mealsite, restored Creston Depot.
Agenda includes: claims; public forum; review hangar contracts for Greg Wurster and Dave Crigger; discuss CIP for fiscal year 2014; discuss ACIP planning exhibit.
Mount Ayr School Board, 6 p.m. Monday, boardroom.
Agenda includes: operations and maintenance plan for safe room; sharing agreement for girls basketball between Diagonal and Mount Ayr; bus bids; early graduation request; Iowa drug and alcohol testing program driver enrollment form; second reading of board policy 401.7 – employee and board policy 507.9 – wellness; personnel; secretaries-treasurer financial report; superintendent's report: timeline for refinancing debt, sewer line and energy audit timeline.
Gibson Memorial Library Board of Trustees will attend the Lincoln School town meeting 6:30 p.m. Monday.
Corning School Board, 7 p.m. Monday, meeting room.
Agenda includes: student council report; Iowa Core report; WSG activities committee report; superintendent: task force on teacher leadership and compensation, district-developed special delivery plan and superintendent network information; prinicpals reports; vehicle bids; Level II investigator; Adams Community Foundation Board member; WGS advisory committee members; John Huggins: fundraiser request instr. lodge 275; policy 407.6 employee early retirement; second reading of school board policy 212-217; school board development: Whose Job Is It? part 2.


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Sunscreen isn't just for beach vacations. The reflection of the sun off the snow strengthens its impact on your skin so SPF is a must. Something we think of so you don't have to.
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"Great Christening gift"
By Darlene Burns
Comments: I ordered this frame for my Godson. I love to give a gift that will have special meaning for years to come. The Christening is next month and I will be so happy to give the frame, in addition to a money gift, so he can have something from his Godparents forever. It is very well made.


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Some product exclusions may apply. See product page details for more information. Clearance items are final sale. Price matching does not apply to this or any promotion. Offer is valid until September 13 at 11:59 PM PST. May not be combined with any other offer/discount (unless specified), including invitation credits, promotional gift cards and/or special discount links. Discount applied upon checkout. Log in to your account to see your savings.
Designed by Arne Jacobsen for the SAS Royal Hotel in Copenhagen, Denmark, the AJ Floor Lamp is a modern classic. The lamp features a circular cutout, which was originally intended to accommodate an ashtray. Without the ashtray, the hole adds litheness and lightness to the design. Featuring an asymmetrical shape and tilt-able head, this lamp is ideal for places where distinct and directed illumination is required.
Uses 1 incandescent 60 W A19 frosted glass lightbulb (not included). Shade length: 12.8". 9' cord. On/off switch located on base.
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Home > Home > Christmas Decorations > Christmas Trees > Pre-Lit Jersey Fraser Tree with Color-Changing Technology
Pre-Lit Jersey Fraser Tree with Color-Changing Technology
White lights change to multi-color with a touch of a button
Get two trees in one!
- 6 ft. tree is pre-lit with 650 lights and measures 55"w x 72"h
- 7.5 ft. tree is pre-lit with 1,000 lights and measures 59"w x 90"h
- 9 ft. tree is pre-lit with 1,500 lights and measures 69"w x 108"h
- Made from durable PE/PVC
- Lights are strung together with a thinner wire so it's hardly visible within the tree
- Hinged construction
- Three position switch: off, multi-color and soft white
- Includes folding metal tree stand, replacement bulbs and fuses
Can't decide between white and multi-colored lights? Now you don't have to! Our Jersey Fraser Hinged Tree is pre-lit with low-voltage LED bulbs that change from a string of soft, all-white to a string of multi-color lights with a touch of a button. Bulbs are specially designed to emit the same warm glow and retain the same appearance of conventional bulbs, and last 25,000 hours compared to the 3,000 hours of conventional bulbs. They are also energy-efficient, using only 1.15 watts of power per 100 lights compared to 44 watts for traditional bulbs.
They look and feel like the real thing.
The full line of color-changing trees feature lush, lifelike foliage and hinged construction so they retain their shape year after year.
Order your pre-lit Jersey Fraser Tree from Brookstone today!
Pre-Lit Jersey Fraser Tree with Color-Changing Technology
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The Stefanel website is owned by Stefanel S.p.A. and managed by Triboo Digitale S.r.l.
Stefanel S.p.A. and Triboo Digitale S.r.l. give fundamental importance to the privacy of their Users. All data provided by those visiting the Site will be processed paying maximum attention and taking all necessary precautions to guarantee their safety, in full compliance with the Italian Personal Data Protection Code rules and regulations.
Informative Note pursuant to Art. 13 of L.D. 196 dated June 30th 2003 - Personal Data Protection Code.
All personal data provided by the Users visiting the Stefanel website (hereafter the "Site" will be processed by the Data Controller, Stefanel S.p.A. with registered office in Ponte di Piave (TV, via Postumia n. 85 (hereafter "Stefanel") and by Triboo Digitale S.r.l., with registered office in Viale Sarca n. 336, Milan, Italy (hereafter "Triboo Digitale S.r.l."), the Data Processor designated by Stefanel S.p.A. to manage the Site and all related services (maintenance, updating, Customer Care services, sending of newsletters etc) and also the co-Controller exclusively for the processing of data as appointed by Stefanel S.p.A. pursuant to the on-line transactions generated by the Site store, using manual and automated systems in compliance with what required by L.D. n. 196 dated June 30th 2003 – Personal Data Protection Code. In particular: to allow web navigation, registration, conclusion and execution of purchase contracts, handle the provision of products and services requested by Users, to provide information and assistance required by Users or, based on a further Information note and consent granted by Users, in order to perform all commercial and sales activities. For these exclusive purposes, User data may be communicated by Stefanel S.p.A to subjects and operators with whom Stefanel SpA and Triboo Digitale S.r.l. have specific contracts (i.e. importers, distributors, dealers, shipping companies, banks and credit institutes, Internet providers), or to Stefanel Group companies or those belonging to their sales and servicing networks (branches, importers, distributors, agents, workshops) which may have their headquarters outside the EU and, in this case the data will be processed exclusively for the above indicated purposes; personal data will be accessed by the Data Processors and the Designated Officers appointed by Stefanel and Triboo Digitale S.r.l. and the above mentioned subjects.
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In order to enable certain functions and achieve higher levels of performance of the Site, the latter uses a cookie system : these are files sent by the Site server and registered via the User's browser on the User 's hard disk, containing information relating to the navigation and activity performed by the User (website pages visited, geographic area, websites which led to this Site, orders placed and completed), which are then relayed from the user's computer to the Site server during any future visits to the Site. The data recorded by cookies does not include, and is not linked, to any User identification data; they do not allow for such identification and cannot be used to identify Users in any manner; they are used solely to speed up the Site functions should the User visit the website again, or to perform statistic based activities or produce reports by processing anonymous or aggregate data. The User is entitled to receive information on the use of cookies, and also set the browser options on his own PC to limit or prevent use of the same. The User can also completely cancel all cookies using the relative functions on his browser programme. If these last two options are enabled on the Browser, the Site may not function correctly.
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In your capacity as the Data Subject, you are granted the rights foreseen by Art. 7 of L.D. n. 196 dated June 30, 2003 and listed below. For further information on how to exercise such rights and for a complete list of the officers designated by Stefanel S.p.A. for the processing of personal data, please send an e-mail to: firstname.lastname@example.com, to the attention of the pro-tempore Administration Manager, or by fax to +39-0422.819.696; Stefanel S.p.A. has appointed Achille Mucci as its Data Processor (email@example.com); when contacting Triboo Digitale S.r.l., users can phone +39-02-64741401, send a mail to firstname.lastname@example.com, or a fax to +39-02-64741491.
In your capacity as the Data Subject, you are granted the rights foreseen by Art. 7 of L.D. n. 196 dated June 30, 2003 and listed above. For further information on how to exercise such rights and for a complete list of the officers designated by Interfashion S.p.A. for the processing of personal data, please send an e-mail to: firstname.lastname@example.com, to the attention of the Administration Manager, or by fax to +39-0541.706801; Interfashion S.p.A. has appointed Gino Trentin as its Data Processor .
Legislative Decree 196 dated June 30, 2003 Personal data protection code
Art. 7 Rights to access personal data and other rights
1. The Data Subject has the right to request confirmation as to whether or not personal data concerning you exists, regardless of their being already recorded, and communication of such data in an intelligible form.
2. In particular, the subject has the right to obtain indications on : a) to receive information on the origin of the personal data; b) on the purpose and processing methods; c) on the logic used in the case where the data is processed using electronic equipment; d) on the personal data of the Controller, the persons in charge and the designated representatives pursuant to Art, 5 paragraph 2; e) the subjects or categories of subjects to whom the personal data may be communicated or who may become aware of such data in their capacity as Designated Representatives for the Country or Persons in Charge of Processing.
3. The Data Subject has the right to obtain indications on : a) updating, rectification or, where interested therein, integration of the data;
b) erasure, anonymisation or blocking of data that have been processed unlawfully, including data whose retention is unnecessary for the purposes for which they have been collected or subsequently processed;
c) certification to the effect that the operations as per letters a) and b) have been notified, as also related to their contents, to the entities to whom or which the data were communicated or disseminated, unless this requirement proves impossible or involves a manifestly disproportionate effort compared with the right that is to be protected.
4. The interested party is entitled to refuse total or partial:
a) on legitimate grounds, to the processing of personal data concerning him/her, even though they are relevant to the purpose of the collection;
b) to the processing of personal data concerning him/her, where it is carried out for the purpose of sending advertising materials or direct selling or else for the performance of market or commercial communication surveys.


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After the Fox (Caccia alla volpe)
With Peter Sellers as star, Neil Simon as screenwriter, and Vittorio DeSica as director, how could After the Fox miss? Miss it did, however--though the film, patchy and inconsistent though it might be, definitely has its moments. Sellers plays an Italian master thief who can't seem to stay out of jail. His latest scheme involves moving $3 million worth of stolen gold bullion from Cairo to Rome. To cover his tracks, Sellers pretends to be a nouvelle vague movie director, filming a crime picture. Britt Ekland, Mrs. Sellers at the time, plays his movie-struck sister. The film is effortlessly stolen by Victor Mature, who is unbearably funny as a vainglorious hasbeen Hollywood star. Director DeSica shows up in the film as himself-at least until all his camera equipment is stolen by Sellers and his partner-in-crime Akim Tamiroff. Never as hilarious as it should have been, After the Fox nonetheless manages a few isolated belly laughs. Outside of Mature's performance, our favorite bit in the film is the final gag: Ze wrong man has escaped! ~ Hal Erickson, All Movie Guide
Cast: Peter Sellers, Victor Mature, Britt Ekland
Produced by: John Bryan
Directed by: Vittorio De Sica
Written by: Neil Simon
Running time: 1hr 42min
Opens: Jan 01, 1966
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KitchenAid Professional HD Stand Mixer
by KitchenAid | Item #: 492500 | Model #: KG25HOXER | (1703) |
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The powerful KitchenAid Professional HD Stand Mixer is capable of far more than just mixing.
The Professional Bowl-Lift Stand Mixer is perfect for heavy, dense mixtures. Burnished metal flat beater, PowerKnead™ Spiral Dough Hook and 6-wire whisk will help you mix, knead and whip ingredients into culinary masterpieces quickly and easily. And for even more versatility, the power hub fits optional attachments from food grinders to pasta makers and more.
|5 quarts
|475 watt motor
|Not specified
|Assorted colors available
|26 lbs.
|Additional Features
|10 speeds
- Direct Drive transmission, all steel gears, all metal construction
- PowerKnead Spiral Dough Hook
- Wire whip
- Flat beater
- Multipurpose attachment hub with hinged hub cover
- Unique mixing action
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Jackie Chan in Shinjuku Incident (San suk si gin)
Action/Adventure, Drama
Set in 1990s Tokyo, the film centers on how the lives of Chinese immigrants in Japan are affected by the Japanese yakuza, or organized crime. When the yakuza threatens his countrymen, Nick realizes that he must do what is right regardless of the consequences.
Cast: Naoto Takenaka, Daniel Wu, Jackie Chan, Bingbing Fan, Jinglei Xu
Produced by: Willie Chan, Solon So
Directed by: Tung-Shing Yee
Written by: Tin Nam Chun, Tung-Shing Yee
Running time: 1hr 58min
Opens: Feb 05, 2010 Limited
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Boston Strong and Boston Wrong
Earlier this week I published an opinion column in USA Today about how the controversy over where to bury â or if to bury â the corpse of a suspected terrorist had turned âBoston Strongâ into âBoston Wrong.â The pride I had felt as a native Bostonian for the heroism demonstrated by so many public officials and ordinarily citizens after the marathon bombings was being eclipsed by shame and embarrassment for those who refuse to treat a dead body as a dead issue.
As I should have expected, the venom and vulgarity directed at the deceased Tsarnaev brother were redirected my way as soon as my words appeared in print. Just because I supported the fundamental decency of a burial, I was supposedly in support of the reprehensible act that the deceased had allegedly committed in life. That Tamerlan Tsarnaev behaved inhumanely apparently meant that we Bostonians had to act in kind -- in a manner that is anything but kind. My expression of compassion arguing that the family be allowed to grieve without protestors chanting for desecration of the remains was met with all sorts of ugliness and profanity, veiled threats and personal attacks, even messages of denunciation sent to my colleagues and co-workers.
Notwithstanding my position on the matter, I do respect the right for anyone to resist and resent the idea of having Tsarnaevâs body buried in our land. It is the disgraceful way in which they demonstrate their point of view that offends me.
I truly understand and appreciate why many folks want nothing to do with the corpse of a man who apparently hated America and our way of life. I recognize the insulting irony to having him remain within our borders, even if underground. That does not, however, justify this less-than-silent minority from turning on their neighbors with such hostility. Fundamental to our way of life is the freedom of expression. No one should be assailed for their words or beliefs so long as they do not threaten public safety. I fail to see how advocating for human decency -- even for someone like Tsarnaev who came nowhere close to that standard -- should threaten anyone.
In the wake of terrorist attacks, it is important that we not forfeit our civil rights, nor our sense of civility. There are people around the world who perceive us as ugly Americans. We should strive not to confirm those unfair stereotypes. If we are to remain a strong city in the strongest nation on the planet, then we should never suspend our morality and compassion just because some now-deceased terrorist did.
For the benefit of those halfway-readers who would interpret my position as sympathy for the devil, let be by crystal clear: I shed no tears for Tamerlan Tsarnaev. My sadness is for how so many Bostonians have responded in the past few days over attempts to bury some skin and bones. Although the man may have been dangerous and evil, the corpse is of no threat to us.
With the news that Tsarnaev's body has been laid to rest somewhere outside of Massachusetts, hopefully this unfortunate battle can be put to rest as well. To me, it seemed beneath us to block efforts to put Tsarnaev in the ground beneath us. But It was certainly beneath us for people to attack neighbors because of their sense of righteousness.
4:00 pm Update: In the few hours since this blog post went live, my e-mail inbox has been graced with no fewer than fifty messages indicating the senderâs agreement with my observations. This, of course, is in addition to the many readers who have posted positive comments online. I wish I could share with you some of the encouraging and thoughtful expressions of human compassion and decency that were sent my way. Many have stated the case far more powerfully that I could have. I plan to acknowledge each one privately, but wanted to make this public statement of gratitude as well. My faith has been fortified: Bostonians Strong. Now that the burial controversy is done, let us get back to the more critical business of protecting our freedoms of speech, ideas and assembly. Were we to yield these precious rights in any way, our enemies would win.
Author's note: You can follow me on twitter at @jamesalanfox or Facebook at Professor James Alan Fox for notifications of new blog postings. Also, you can find me on the Web at www.jamesalanfox.com or contact me by e-mail at email@example.com.
The author is solely responsible for the content.


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All eyes were on southern Spain last week as the Circuito de Jerez played host to the first of three weeks of testing ahead of the 2013 Formula 1® season.
The four days of action are always keenly anticipated as they present the first opportunities of the new racing year to witness the full range of updated vehicles in action. The week in Jerez also provides an early chance for fans to familiarise themselves with drivers new to F1® , or simply new to their teams after switching in the off-season.
But aside from all the novelty, what does the first week of testing actually tell us about the fortunes of teams and their drivers ahead of the new campaign?
An honest answer to that question would probably be, "not as much as you might think." Such are the myriad variables involved in the four days in Andalucia it is almost impossible to gauge their relevance to the Drivers' or Constructors' championships. Fuel loads fluctuate, tyre choice varies, and in some cases even test drivers are employed, obfuscating meaningful attempts to measure and compare season readiness.
Though accurate comparisons across manufacturers may be difficult to make, there is still plenty to take from these early-season expeditions. Below is a flavour of what we've learned so far from 2013 testing.
Red Bull
Red Bull's appetite for silverware shows no sign of diminishing following the first run out of the RB9. Only minor modifications to the championship winning RB8 have been made and both Sebastian Vettel and Mark Webber appear pleased with the outcome.
Reliability and consistency were Red Bull's keywords in Jerez with both drivers praising their vehicle after clocking up around 200 laps each. This provided team engineers with plenty of valuable data, in particular about how the RB9 operates on the new Pirelli tyres. Race Engineering Co-ordinator Andy Damerum was happy with his week's work, suggesting a solid foundation had been set and that more instructive information could now be gleaned from Barcelona testing.
It might be a different colour to 2012, but the Red Bull still looks the car to beat in 2013.
Ferrari's week in Spain is amongst the most difficult to evaluate. The absence of lead driver Fernando Alonso obviously complicates matters, especially with test driver Pedro de la Rosa suffering a gearbox issue on the final morning. However, that is all offset by Felipe Massa setting the fastest lap-time of the week by any driver. The Brazilian left Jerez impressed by the new F138, indicating it was an easier car to drive, more stable and better balanced compared to the F2012.
Some promising signs then for the Italian giants and their army of followers, but a real picture of Ferrari's performance will not emerge until after Barcelona.
McLaren's week got off to a difficult start with a fuel pump problem limiting Jenson Button's opportunities in the MP4-28. With mechanical dependability a major concern in 2012, doubts have again been raised as to whether arguably the fastest car on the grid is capable of finishing enough races to secure a championship. Things improved thereafter though with Button recording lap times described by Felipe Massa as "incredible" and Sergio Perez looking at ease in his new surroundings.
There's no question McLaren has the performance to compete in 2013 but the past week in Jerez has not put lingering reliability fears to bed.
Kimi Raikkonen ended the week of testing as the fastest driver, with team-mate Romain Grosjean the fourth quickest overall for the week – so something must be going right with Lotus's E21. As notoriously unreliable as the first week of testing can prove, Lotus appears to have as much to cheer as any team departing Jerez.
F1® fans should keep a close eye on the Enstone outfit for the remainder of the pre-season to establish if there is any measurable improvement to back up this positive early form.
The Silver Arrows continued their launch theme of expectation management following a mixed week of testing. The opening two days for Nico Rosberg and new-boy Lewis Hamilton were disappointingly brief, courtesy of front-break and wiring-loom problems. Once up and running the F1-W04 looked competitive without suggesting it was yet a rival to the established front-runners.
Hamilton acknowledged the Mercedes offering was less than he was accustomed to at McLaren, saying, "I definitely notice the difference, but it is nowhere near as bad as it could be."
With Hamilton and Ross Brawn already talking about developing the 2014 model, Mercedes will have to impress in Barcelona testing to give any indication they are worth close attention in 2013.
The rest
Predictably, none of the remaining teams put in performances that would suggest a marked difference in results in 2013. There were a few encouraging displays though, notably from F1® rookies Jules Bianchi and Esteban Guttierez.
Bianchi, a test driver for Force India, recorded the third-fastest time of the week, doing his chances of a regular drive no harm at all. Gutierrez, driving alongside Nico Hulkenberg for Sauber this year, recorded the sixth-fastest time overall. Just 21, the Mexican is the latest prodigious talent to cut his teeth at the pinnacle of motorsport.


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Bank In The Form Of A Pig is part of the Reality series designed by Harry Allen for Areaware. This bank is produced by casting polyester resin in a silicone mold from a real pig (who lost his life from natural causes). The result is a highly detailed, realistic sculpture—who we're sure is happy to hold a lot of bread.
Cork stopper included. Designer Harry Allen strives to make your life thoughtful and beautiful with expertise in industrial design, interior design graphics, and identity development.
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petsupplies.com > Clearance Pet Supplies > Cat > Item # 400487
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Savvy Tabby Sparkle Paw Cat Collar
This Savvy Tabby Sparkle Paw Cat Collar is high on style and is crafted to offer comfort to your pet. Made from faux leather, this cat collar offers durability for long-lasting usage. Detailed with embossed silver, metallic paw-print design, this cat collar flaunts a charming, attractive appeal. These shiny accents give a perfect contrast to the matte finish of the leather for a chic look. The reinforced stitching on this cat collar adds to its durability. Plastic clip in the cat collar allows easy adjustment of the size and ensures a secure, yet comfortable fit for pets. A matching jingle bell adds to the charm of this cat collar while breakaway buckles enhance the safety of this accessory.
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HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases specifically Ras wildtype
Ras and related small molecular weight GTPases function in the regulation of signaling and cell growth, and collectively serve to control cell proliferation, differentiation and apoptosis [Tekai et al. 2001; Wennerberg et al. 2005]. Ras family members (Ras, Rap, Ral, Rheb among others) are recognized their control of growth factor receptor signaling cascades. When mutant or hyperactivated, Ras more ..
Tested Compounds
Tested Compounds
Tested Substances
Tested Substances
Related BioAssays
Related BioAssays
BioActive Compounds: 267
Related Experiments
University of New Mexico Assay Overview:
Assay Support: NIH I RO3 MH081231-01
HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases
PI: Angela Wandinger-Ness, Ph.D.
Co-PI: Larry Sklar, Ph.D.
Assay Development: Zurab Surviladze, Ph.D.
Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller
Target Team Leader for the Center: Larry Sklar (email@example.com)
Assay Background and Significance:
Ras and related small molecular weight GTPases function in the regulation of signaling and cell growth, and collectively serve to control cell proliferation, differentiation and apoptosis [Tekai et al. 2001; Wennerberg et al. 2005]. Ras family members (Ras, Rap, Ral, Rheb among others) are recognized their control of growth factor receptor signaling cascades. When mutant or hyperactivated, Ras family members contribute contribute to ongogenesis. The Ras-related GTPases are divided into four subfamilies with the Rab proteins regulating membrane transport, Rho proteins (including Rac and Cdc 42) regulating cytoskeletal rearrangements and responses to signaling, Arf/Sar proteins regulating membrane and microtubule dynamics as well as protein transport, and Ran protein controlling nucleocytoplasmic transport. Ran is the subject of an independent screening initiative (Weiss, K. UC Berkeley) and Arf family members are membrane anchored via their N-termini in contrast to other family members, which are anchored via their C-termini [Kahn et al. 2006]. This project focuses on representative Ras, Rho, and Rab family members to validate the approach for the identification of new chemical compounds with novel therapeutic potential in cell signaling and growth control.
Ras and Ras-related GTPase functions are tightly regulated, and dysregulation is causal in a wide variety of human diseases. Proper functioning of Ras and Ras-related GTPases is regulated at the level of localization and nucleotide binding and hydrolysis. Ras mutations resulting in impaired GTP hydrolysis and plasma membrane hyperactivation are linked to many human cancers [Farnsworth et al. 1991; Sukumar et al. 1983; Taparowski et al. 1982; Boylan et al. 1990; Hruban et al. 2004; Abrams et al. 1996]. Point mutations in the Rab and Rho GTPases are also causal in diverse human diseases affecting pigmentation, immune, and neurologic functions [Houlden et al. 2004; Verhoeven et al 2003; Williams et al. 2000; Bahaderan et al. 2003; and preliminary findings]. Rab and Rho mutants identified in human disease act as dominant negatives either due to a failure to bind GTP or due to inappropriate coupling of the active proteins with downstream effectors. To date, inhibition of Ras and Ras-related proteins has relied largely on altering membrane recruitment with various drugs affecting prenylation [Morgillo-Flee HY, 2006; Russell RG, 2006; Park, et al. 2002]. Generally, Ras proteins must be farnesylated for proper membrane localization, while Rab and Rho proteins are geranylated. Such strategies lack specificity and are problematic because each of these prenylation machineries is required for the proper function of many Ras superfamily members. Rational drug design has only recently been applied to identify the first two small molecule inhibitors of Rho GTPase family members [Gao, et al. 2004; Nassar et al. 2006]. Therefore, broadly testing the Ras-related GTPases as targets for small molecule inhibitors and activators is expected to identify new classes of compounds that may be useful in the treatment of human disease, as well as in unraveling the molecular details of how Ras-related GTPases function.
The assay described here is a no-wash fluorescent GTP-binding assay adapted to multiplexed, high-throughput measurements whereby multiple GTPases are simultaneously screened against the MLSCN library. The specificity is based on the observation that individual GTPases including wt and activated forms exhibit measurably distinct affinities for Bodipy-FI-GTP vs GTP. The multiplex assay involves the binding of fluorescent GTP to G protein-GST fusion proteins on GSH beads. A set of six G proteins (Rac 1 wt, Rab7 wt, Rac 1 activated, Ras wt, Rab 2 wt., CDC wt) are arrayed under conditions of divalent molecule depletion in the assay reported here.
Bead sets are coated with individual GST-small G proteins, blocked with 0.1% BSA in Buffer (0.01% NP-40; 30mM HEPES pH 7.5; 100mM KCl; 20mM NaCl; 1mM EDTA) and incubated overnight at 4 degrees C. Beads are washed in wash buffer (0.1% BSA and 1mM DTT). The different bead sets, acquired from Duke Scientific, have similar size (~ 4 microm diameter) however they are distinguished by varied magnitude of emission at 665 +/-10 nm with excitation at 635 nm.
The assay is conducted in 384-well microplates in a total well volume of 10.1 microliters (5 microliters of bead mixture, 0.1 microliters of test compound, and 5 microliters of 200nM Bodipy-FL-GTP in buffer containing BSA and DTT for a final concentration of GTP of 100nM). Positive Controls, which contains bead mixture and fluorescent GTP but no test compound, are located in columns 1 and 2 on plate. Negative Controls, bead mixture with fluorescent GTP and 0.5 mM unlabeled GTP, are collected from a separate test tube. Plates are placed on rotators and incubated for 40-45 minutes at 4 degrees C.
Sample analysis is conducted with the HyperCyt(R) high throughput flow cytometry platform [Kuckuck, et al., 2001; Ramirez, et al., 2003]. Flow cytometric data of light scatter and fluorescence emission at 530 +/- 20 nm (FL1) and 665 +/- 10 nm (FL8) are collected via Cyan (Dako). The resulting time-resolved single data file per plate is analyzed by IDLQuery software to determine the compound activity in each well. Gating based on FL8 emission distinguishes the beads coated with different proteins, and the median fluorescence per bead population is calculated.
In order to get a significant measurement of the effect by a compound on a particular protein, 25 beads with that particular protein bound is the minimum number of beads to be collected from a well. When less than 25 beads are counted, the result for that protein is considered missing. Compounds from missing wells is given PUBCHEM_ACTIVITY_OUTCOME = 4, and are automatically assigned a PUBCHEM_ACTIVITY_SCORE of 0. In this set of 194,738 compounds, for RasWT there are only 63 missing compounds.
When the measured emission is potentially attributed to the innate fluorescence of the compound, these compounds are flagged as "Possible Green Fluorescent Compound" (see column titled PUBCHEM_ASSAYDATA_COMMENT) and the results from a fluorescent compound is considered to be 'inconclusive' (PUBCHEM_ACTIVITY_OUTCOME = 3, PUBCHEM_ACTIVITY_SCORE = 0). Assessment of fluorescent compound is made by comparing the influence of compound fluorescence on all the proteins in one well. Difference between Sample fluorescence and Positive Control fluorescence are calculated for all the different proteins in a well. The following equation describes this difference (CompoundFLinRacWT) for RacWT;
CompoundFLinRacWT = RacWTSampleFL - PCntrlRacWTFL
with RacWTSampleFL being the Sample fluorescence and PCntrlRacWTFL the Positive Control fluorescence of RacWT coated beads. Next, the coefficient of variation (CV) of all these CompoundFL from the different protein beads (RacWT, Rab7, RacACT, RasWT, Rab2, Cdc) in the same well are calculated. If the CV was less than 30%, meaning the compound attributed fluorescence was very similar between all the different proteins, then the compound was flagged as a potential fluorescent compound. In this set of 194,738 compounds, for RasWT there are only 4,084 fluorescent compounds.
Due to potential systematic trends in data over the entire plate (whole plate trends), normalization is utilized to calculate the percent activity of the test compound. Whole plate trends of the positive controls are evaluated by linear regression. Then due to the plate location of a sample, a calculated Positive Control value (notated with WPcalc) is utilized for calculating % activity normalized by whole plate:
% Activity = 100 x (SampleFL - NCntrl)/(PCntrl_WPcalc -NCntrl)
where all variables are Median Fluorescence Intensity associated with the bead set bound with a specific protein. SampleFL is for beads in wells containing test compound, PCntrl_WPcalc is the well-specific, calculated value based on whole plate linear regression of wells without test compounds, and NCntrl is for measurement in presence 0.5 mM unlabeled GTP. Baseline of % Activity is 100%.
Maximum PUBCHEM_ACTIVITY_SCORE of 100 was given for the primary screening. The absolute difference between baseline and %Activity was used to calculate PUBCHEM_ACTIVITY_SCORE:
PUBCHEM_ACTIVITY_SCORE = |100% - %Activity|
A compound was considered "Active" if the change in %Activity was greater than 20% from baseline (i.e., PUBCHEM_ACTIVITY_SCORE >=20%). PUBCHEM_ACTIVITY_OUTCOME is indicated as 2 for "Active" and 1 for "Non-active".
For "Active" compounds, the type of activity is indicated in column entitled "ActivityType". "Inhibitor" is a compound with <80% Activity and "Activator" is a compound with >120% Activity.
Average Zprime for this screen was 0.90 +/- 0.03.
Keywords: NIH Roadmap, NMMLSC, high throughput flow cytometry, GTPase, multiplex bead-based screening
Result Definitions
** Test Concentration.
Data Table (Concise)


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The Community of Disc Golfers and About All Things Disc Golf
At the USDGC this year the TD wanted to have a rules exception that would create a putting circle around the basket 1.5 meters out. If your disc landed within this radius then you would have to mark it somewhere along the circle creating a 5 foot putt instead of a drop in the reasoning being that a drop in is not a good golf shot and you can merely drop it in even place it in without a throw, that is somehow seen as wrong anyway what an absurd idea, really dumb, anyone care to defend the new proposed rule?
Views: 42
© 2015 Created by Terry "the Pirate" Calhoun.


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Helene, a small and faint moon of Saturn, is referred to as a Trojan satellite because it orbits Saturn in the Lagrange point of the larger moon Dione.
Helene was discovered 1March 1980 during the Earth ring-plane crossing by J. Lecacheux and others.
How Helene Got its Name:
John Herschel suggested that the moons of Saturn be associated with mythical brothers and sisters of Kronus. (Kronus is the equivalent of the Roman god Saturn in Greek mythology.) The International Astronomical Union now controls the official naming of astronomical bodies.
Originally designated S/1980 S6, Helene is named after the granddaughter of Kronus and is the sister of Polydeuces. Helene was born out of an egg since Zeus took the shape of a swan when he raped her mother Leda. The account of this engendering is retold in the poem "Leda and the Swan" by William Butler Yeats. This same figure in Greek mythology was the cause of the Trojan War.


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Mystical India
Mystical and spiritual, India is a land of rich contrasts. Explore its multifaceted culture as you travel through the classic Golden Triangle and east to Varanasi, stopping in Kalakho where local Meena tribespeople invite you into their homes.
Tour Details
Of all the overseas trips we've taken, this trip ranks right at the top. Smithsonian did an outstanding job of balancing sites and activities with learning. We saw and did so much but we learned even more. We'll always remember India."
Ron Z.
I have wanted to visit India all my life and, therefore, had very high expectations. This trip far exceeded all those expectations. It was everything I hoped for and more!"
Brenda S.
India is not only mystical, but it is memorable, and magic. An initial visit only whets one's desire to return to experience the unique physical and spiritual senses over and over again."
Kenneth N.
I am so happy I saw India with the Smithsonian. It has such a rich and extraordinary history, and Smithsonian was the way to go!"
Previous Journeys Traveler
- A Tailor-Made Journey to India and Nepal
- The Forbidden City: Official Spaces and Private Quarters
- Journey from Bangkok to Bali: Following the Spirits
- Haydn Seek
Save $200 *per person when you book by September 12, 2015! Use promotion code LASTCALL online or when speaking to our travel specialists to secure these savings.
- Oct. 22, 2015
- Nov. 26, 2015
*Offer valid on new bookings only.
Mystical and spiritual, chaotic and confounding, India is a land rich in contrasts. Join us to explore northern India's multifaceted culture, history, and traditions as we travel through the classic Golden Triangle and east to Varanasi.
In sprawling Delhi, tour both Old and New Delhi and ride a rickshaw through colorful Chandni Chowk market. In the "pink city" of Jaipur, marvel at historic forts, royal palaces, and gardens built by maharajas. Learn about the ancient discipline of yoga at a private demonstration and visit the nearby village of Sanganer to watch craftsmen work at traditional block printing and papermaking. During game drives in Ranthambore National Park look for Bengal tiger, monkey, langur, and exotic birds, and join a camel safari in Kalakho, where local Meena tribespeople invite you into their homes. In Agra, visit the majestic Taj Mahal and take an Indian cooking lesson. Conclude your journey in the holy city of Varanasi to witness the powerful spiritual rituals along the Ganges River.
Days 1-2 — U.S. and Delhi
Depart the U.S. for Delhi on individual flights. Upon arrival late in the evening, transfer to your hotel.
Days 3-4 — Delhi
Built by a succession of empires, Delhi is comprised of many cities. Explore both Old and New Delhi beginning with an afternoon tour of the 16th-century Tomb of Humayun, Qutab Minar, and India Gate. The next day your exploration of Old Delhi begins at the Gandhi Memorial and continues with many highlights, including Lal Qila, the Red Fort complex of palaces where we stop for photos; Jama Masjid, India's largest mosque dating to 1656; a rickshaw ride through Chandi Chowk market; a Sikh temple; and a tour of the National Museum. (2B,2D)
Day 5 — Jaipur
Travel by coach to the "pink city" of Jaipur, one of the great cities of the Rajput, with historic forts, palaces, and gardens. Upon arrival late in the afternoon, settle into your hotel, once a Maharajah's palace. (B,L,D)
Day 6 — Jaipur
Explore the city's rich history beginning with the stunning Hawa Mahal, or "Palace of the Winds", an elaborate facade of carved pink sandstone. Tour the famous Amber Fort, a Rajput capital from 1037 to 1728 and considered the pinnacle of Rajput architecture. Visit an open-air observatory, then view some of the Maharajah's magnificent art collections at the City Palace Museum. (B,L)
Day 7 — Jaipur and Sanganer
Early this morning, enjoy a traditional yoga demonstration and chanting. In the nearby village of Sanganer, learn about traditional block printing and handmade paper industries, then experience the colorful sights and sounds of a local market. Enjoy an invitation to be dinner guests in the home of a multi-generational Rajasthan family. (B,D)
Days 8-9 — Ranthambore
Once the hunting ground of the Maharajah of Jaipur, Ranthambore National Park is now a 512-square-mile nature preserve that is home to hundreds of species of birds, reptiles, mammals— and of course, the endangered Bengal tiger. Arrive in time for an afternoon game drive, and enjoy additional game drives the next day. (Tiger and leopard sightings are not guaranteed.) (2B,2L,2D)
Day 10 — Kalakho
Early today depart for Kalakho and the Dera Village Retreat. Enjoy an afternoon camel safari to meet the local Meena tribespeople in their homes. (B,L,D)
Days 11-12 — Abhaneri and Agra
Travel to the village of Abhaneri to see the fortified Chand Boari stepwell (c. 800), whose 3,500 steps descend some 13 stories into the ground. Arrive in Agra in the early afternoon. The next morning, tour the Taj Mahal, the magnificent tomb built by Emperor Shah Jahan in memory of his beloved wife. After an Indian cuisine cooking lesson and lunch, visit the imposing Agra Fort, a vast complex that served for generations as the seat of Mughal power. (2B,1L,1D)
Day 13 – Varanasi
Depart by coach this morning for Delhi, where you board a late afternoon flight to Varanasi, the holiest city in Hinduism. Dinner tonight is at the hotel. (B,L,D)
Day 14 – Varanasi and Sarnath
Today visit nearby Sarnath, where the Buddha first taught after achieving enlightenment. One of Buddhism's four holiest sites, Sarnath draws pilgrims from the world over. See some of the temples and stupas then tour Sarnath Museum, housing a superb collection of Buddhist artifacts from excavations here. Highlights include a statue of the Buddha turning the wheel of law, and the celebrated Lion Capital of Emperor Ashoka, dating to 250 B.C. Back in Varanasi tonight attend a traditional aarti offering ceremony during a cruise along the Ganges. (B,L)
Day 15 – Varanasi
Early this morning return to the Ganges, where Hindu pilgrims perform their rituals along the ghats (steps) leading to the river. Visit several of the important ghats by boat as you experience the spiritual mystique of these hallowed waters. Enjoy a private Kathak dance performance, then celebrate your journey with a farewell dinner. (B,D)
Days 16-17 – Delhi and Return to the U.S.
Fly to Delhi this afternoon and depart early the next morning for the U.S. on individual flights. (B)


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//Mixed media
Girls Names "A Hunger Artist" (video) (Singles Going Steady) // Music
Carrie Underwood "Smoke Break" (video) (Singles Going Steady) // Music
//Pm Picks
Country Fried Rock: Drivin' N' Cryin' to Be Inducted into the Georgia Music Hall of Fame
// Sound Affects
""If Drivin' N' Cryin' sounded as good in the '80s as we do now, we could have been as big as Cinderella." -- Kevn KinneyREAD the article


text: Pope Benedict XVI waded deep into U.S. campaign politics Friday, urging visiting U.S. bishops to beef up their teaching about the evils of premarital sex and cohabitation, and denouncing what he called the "powerful" gay marriage lobby in America. Benedict has long championed traditional marriage between man and woman, as well as opposition to premarital sex and fidelity within marriage. But his strong comments to visiting U.S. bishops took on particular significance given the culture wars that have erupted in the U.S. this campaign season.http://www.cbsnews.com/8301-202_162-...ctionContent.1


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Kosovo Liberation Army guerrillas believed they were fighting for freedom, but 14 years after the war with Serbian forces, some of Pristina's 'heroes' are struggling for survival.
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Follow me @LandShark_13
Weak Sauce!
°˖✧◝(⁰▿⁰)◜✧˖° !Dolphins have more fun! °˖✧◝(⁰▿⁰)◜✧˖°
Bill Belichick on "putting the tape on"
Thanks guys I love my phins and will die sticking up for them, especially when the jets are involved
**** the jets
Surprised to see your mother letting you post on a weekend. Oh and die in a fire. Please.
Junc was running his mouth all week...enough with this class act BS. He'd be touting Sanchez as the 2nd coming had they won.


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When O.J. Simpson was on trial for the murder of his wife, he could really have used a best friend. And in Ryan Murphy's true-crime drama�
SpaceX and Tesla CEO Elon Musk's grand idea for an 800-mph above-ground transit system is taking it's first steps to become a reality.
The CWC Lady Bulldogs dominated all phases of the game and defeated the Goreville Lady Blackcats 50-28 Thursday night for their first road victory of the season.
An Accident involving a black SUV and a semi occurred yesterday, December 18, 2014 at approximately 1:20.
At least these Sony hacks were good for business for someone.�On Monday, USA Network ordered to series�Mr.
When NBC's gruesome psychological horror�Hannibal�returns for Season 3, it'll bring with it a new...
Hillary Clinton's circle wants to be quoted yawning at Jeb Bush, a sure sign of concern that he could beat her in 2016.
Valerie Anne (Heil) Carter, 60, of Carmi went home to celebrate Christmas with Jesus on December 18, 2014 at 3:20 p.m.
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QuickSilver Deluxe Lever Style Corkscrew 4 Piece Gift Set
A must have for wine enthusiasts!
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The sleek, ergonomic chrome design is die-cast and zinc-plated to provide the extra weight needed for better leverage. Lift the top handle up and over, place the corkscrew over the top of the bottle on top of the cork, grip the handles and pull the top handle back, then lift the top handle up and over again and out comes your cork. Strong non-stick coated screw. This corkscrew is not meant for use with synthetic corks.
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QuickSilver Deluxe Lever Style Corkscrew 4 Piece Gift Set
Reviewed by 1 customer
- It Removes Cork
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Best Uses
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QuickSilver Deluxe Lever Style Corkscrew 4 Piece Gift Set
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April 18, 1947 - Feb. 18, 2014
Musician, producer, teacher and director William "Billy" Adair, a centerpiece of Nashville's jazz community who led the Blair Big Band at Vanderbilt University's Blair School of Music, died today of melanoma cancer at Vanderbilt University Medical Center. He was 66.
A masterful multi-instrumentalist who worked onstage and in the studio with artists including Brenda Lee, The Oak Ridge Boys, Waylon Jennings, Ronnie Milsap, George Strait and Alabama (he played on the latter band's top-charting single, "The Closer You Get"), Mr. Adair was also a broadcast music producer who won more than 150 awards for composing and arranging advertisements.
In Nashville, he was known less for his resume than for his roles as an advocate, an enthusiast and an instructor: He bolstered jazz awareness and visibility, and he brought jazz to a Blair campus that had been primarily concerned with classical music.


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Cover up the all-important sensor on your α NEX camera with this handy body cap. It features a snug fit for optimal protection from dust and accidental fingerprints.
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Oversized Steamer Trunk Storage Locker with Tray
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Great footlocker storage with maximum style.
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Oversized Steamer Trunk Storage Locker with Tray
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While earning a business administration degree at Villanova University, Robert J. McCarthy started waiting tables at a restaurant inside a Philadelphia-area Marriott hotel in 1975.
Little did he know that Marriott would be the only company he would work for in a 38-year career.
McCarthy, the Bethesda hotel giant's COO, will retire in February, company officials said this week. The 60-year-old executive has held a wide variety of positions within the company throughout his career.
"Bob has been an admired business leader, sought-after mentor and trusted colleague, both at Marriott and in the hospitality community," Marriott CEO Arne Sorenson said in a statement. "Bill Marriott has often noted Bob's career success as a credit to his hard work, but also as an example of the vitality of our company culture that promotes opportunity."
From the waiter position, McCarthy advanced to become regional director of sales and marketing for Marriott Hotels, Resorts and Suites by 1982. He became a vice president for operations and marketing for the Fairfield Inn and Courtyard brands in 1991, then senior vice president for the Northeast region of Marriott Lodging in 1995.
McCarthy moved up to executive vice president for operations planning and support for Marriott Lodging in 2000, when the company operated or franchised about 2,000 properties. That number has almost doubled since then. He eventually became group president across the Americas and global lodging services, overseeing operations at more than 3,000 hotels and 120,000 employees. McCarthy has remained active in his alma mater, as he was appointed to Villanova's board of trustees earlier this year. He formerly was a member of the Dean's Advisory Board at the Villanova University School of Business and the Cornell University School of Hotel Administration.
Last year, McCarthy, whom a spokeswoman said was unavailable for comment, made $804,403 in salary and total compensation of $4.4 million, which included more that $2 million in stock awards and options, according to Marriott's most recent proxy statement. The compensation was 22 percent higher than in 2011.
Rather than appoint another COO right away, Marriott plans to give several other executives broader responsibilities. Dave Grissen, group president, will focus on global operations and oversee the Americas.
Stephanie Linnartz, executive vice president and chief brand marketing and commercial officer, will assume leadership of technology.


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The 2012 Hershey Track Meet will take place at 5 p.m. April 23 at Heavener's Harvey Stadium.
Registration forms and a copy of child's birth certificate can be dropped off or mailed to Boys and Girls Club of LeFlore County at 205 Dewey, Suite 6, Poteau, OK 74953. Application deadline is Wednesday. On-the-site applications will not be accepted.
Events will include the 50-meter dash, 100-meter dash, 200-meter dash, 400-meter dash, 800-meter run, 1,600-meter run, 400-meter relay, standing long jump, and softball throw.
Those placing high enough will qualify for the state meet June 30 at the University of Oklahoma in Norman to become a regional-meet and/or a national-meet qualifier.
The National Hershey Track Meet will be Aug. 4 in Hershey, Pa.
For additional information, call Terri Wallace at (918) 647-7136 or (804) 832-3473.
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The Pocola High School football program is sponsoring a fundraiser in connection with Riverside Autoplex and Ford Motor Company.
Starting at 9 a.m. Saturday at Pocola High School, people can come and take a free test-drive of Ford vehicles, and Ford Motor Company will donate up to $6,000 to Pocola High School.
There will be a chance to win a 2013 Ford Escape.
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Girls are needed to play for an age 12-and-younger softball team in the Green Country Softball League. Games will take place at the Poteau Area Recreational Complex. The team will practice in Cameron. For additional information or to register, call Mike House at (918) 658-5510.
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The Poteau Lady Pirates softball program will have an age 6-7 coach-pitch league beginning in May and end its season in June.
Players must have been age 7 by Jan. 1, 2012, or must turn age 6 by Jan. 1, 2013. The entry fee is $150 per team, and the league is open to six teams. For additional information, call Kathy Nye at (918) 649-4326 or email to email@example.com.
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The Poteau Kiwanis Spring Scramble Golf Tournament will begin at 9 a.m. April 28 at Choctaw Country Club.
The tournament will benefit Boys and Girls Club of LeFlore County and LeFlore County Youth Services.
Entry fee is $50 per person, if paid before the tournament date, $60 the date of the tournament or $200 per team.
Tournament hole sponsorships are available for $100. Businesses or individuals can sponsor a team and hole for $250. For more information, call Lyle Whitworth at (918) 413-2929, Ryan Adams at (918) 649-7846, Craig Hall at (918) 649-4712 or Choctaw Country Club at (918) 647-3488.
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The first annual Masonic Tournament will take place April 20-21 at the Poteau Area Recreational Complex. There will be a T-ball tournament and an age-8-and-younger softball tournament.
The entry is $75 per team with a three-game guarantee. The championship team in the age 8-and-younger softball tournament will receive team bat bags. For additional information, call Mike Womack at (479) 806-2438 or Shannon Stubbs at (918) 647-6069.
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The 16th Annual Big Mac Three-Course Challenge will be May 4-6 at three different courses, beginning with Wolf Ridge Golf Course on May 4. Day 2's action will be at McAlester Country Club on May 5 and Arrowhead Resort Golf Course on May 6.
Entry fee for the event is $375 per two-man team, which includes three days of green fees, mulligans and a dinner on May 5 at McAlester Country Club.
The winning team will earn $1,000, with $800 to the runner-up team and $600 for third place.
Each par-3 holes will pay off $100 each for the closest to the hole winner, while there will be a longest drive winner each day who will earn $100 for the honor. For additional information, call (918) 647-2582.
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The Brittney Cox Memorial Golf Tournament will begin at 7 a.m. May 5 at Wolf Ridge Golf Course. The tournament will conclude when darkness falls. Lunch will be provided.
The entry fee for the two-man scramble is $135 per team. Hole sponsorships are available at $100 per hole.
Deadline to purchase a hole sponsorship will be Sunday. All proceeds will benefit Poteau High School.
For additional information, call Brenda Sanders at (918) 649-4039 or Cody Cox at (918) 649-7980.
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The third annual Village 2 Village Golf Tournament will begin with a shotgun start at 8 a.m. June 23 at Wolf Ridge Golf Course.
Entry fee for the four-man scramble is $280 per team. After June 16, the entry fee will increase to $320. Entry fee includes green fees, cart and a barbecue lunch.
Mulligans will be available at $5 each with a limit of one per nine holes.
There will be a putting contest, with the entry fee being $5 per putt or $10 for three putts.
The first hole-in-one winner will win a car. For additional information, call (479) 785-3575 or go online to www.v2vClassic.com.
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The 10th annual Carl Albert State College Scholarship Golf Scramble will begin at 10 a.m. May 12 at Shadow Creek Country Club in Sallisaw.
Registration will begin at 9 a.m. May 12.
Entry fee for the four-person scramble is $50 per person or $200 per team.
The winning team will win $600, with the second-place team getting $450 and a wild-card drawing will take place with the lucky team earning $300.
There will be prizes awarded for the longest drive, closest to the hole and holes-in one. For additional information or to pre-register, call Paul Marshall at (918) 776-9242.
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The Boys and Girls Club of McAlester will put on a competitive baseball and softball league.
It is believed sports encourages a healthy lifestyle in our youth, which is a rising concern to many.
Sports also promotes high self esteem in children that will benefit them in many aspects. For additional information, call the Boys and Girls Club of McAlester Athletic Department at (918) 426-5144.
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The Satchel Paige All-Stars Travel Baseball Team is looking for youth baseball players ages 13-14, 15-16 and 17-18 years old that would like to be a part of our 2012 Summer Travel Baseball Team that will include trips to Cincinnati, Omaha, Neb., Springfield, Mo., Orlando, Fla., and Puerto Rico. All interested players should contact Coach Mike Mayden for registration information at (773) 744-1040 or email to www.whsn.org.


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Location: Located at the heart of Bur Dubai's commercial district and in close proximity to the Dubai World Trade Centre and BurJuman shopping mall. Dubai International Airport is only 10 kilometres away.
Closest Metro Station: Al Karama Station.
Restaurants: Selection of restaurants and bars.
Facilities: Internet facilities, fully-equipped health club and rooftop swimming pool.
Guests Rooms: 282 guest rooms and suites.
Note: 24 hours check-in/check-out.
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