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Cisco AS5400XM Series
The Cisco AS5400XM Universal Gateway offers unparalleled capacity and the flexibility to deploy advanced voice, fax, and remote access services in two rack units (RU). With more than 24 million voice gateway ports deployed, Cisco is a proven leader in product innovation for packet telephony services. Cisco Systems customers worldwide have proven that Cisco AS5000 universal gateways provide the flexibility, scale, reliability, and suite of services required to meet market demands.
The Cisco AS5400XM Universal Gateway doubles the performance of the Cisco AS5400HPX and delivers significant memory enhancements to offer high performance and high reliability in a compact, modular design..It easily supports public switched telephone network signaling, gateway signaling, voice codec, fax, VoiceXML, RADIUS, Tool Command Language, and interactive voice response.
Features of the AS5400XM make it ideal for many network deployment architectures:
* High density to handle increased traffic
* Low power consumption to reduce cost
* High-density packet voice/fax digital signal processor (DSP) to provide greater price/performance, higher density, and expanded codec and feature support for intelligent packet voice services
* Universal port DSP feature cards to support remote access services in combination with voice and fax applications
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You can easily migrate from the AS5400 or AS5400HPX to the AS5400XM to achieve improved performance. And because your current applications will run at a lower CPU utilization you will be able to
* Increase SNMP monitoring or logging to improve network management
* Raise the performance of VoiceXML, voicemail, and prepaid calling card services
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text:
Man in the Mirror's Patrick Morley says the whole concept of traditional men's ministry has a fatal design flaw. Only a small percentage of your men will join a ministry that is for "men only." read more
Page 75 of 100
Biological Changes Affect New Fathers, Too
Studies have revealed that new fathers, in addition to mothers, experience high levels of the hormone oxytocin in the first few months of parenting. read more
Fathers, Speak Life To Your Family
Our words can have a powerful impact on a child in the positive. They can have a tremendous negative impact as well. read more
Let Sex Be God's Gift, Not a Worldly Curse
Sex is one of the most powerful gifts He's given us. But when misused or abused, it can become a tool for terrible destruction. read more
God Loves a Selfless, Obedient Man
John the Baptist epitomized a selfless man of God as he prepared the way for Jesus. read more
Are You a Man or an Ostrich?
The fact is, you are going to face challenges. The question is whether or not you will confront them head-on or drop your head and put it in the sand. read more
Wisdom Keeps a Man Out of Trouble
God blesses those who seek Him. He will grant wisdom to those who ask. read more
Leave and Cleave, But Stay in Touch
Getting married and having your own family is a rite of passage in life. But that doesn't mean you can't continue to have a great relationship with your parents. read more
Don't Allow Touch Deprivation to Creep Into Your Marriage
Psychologists generally agree that as humans, we need—not just want—physical touch. read more
10 Life Lessons From Coach John Wooden
The legendary John Wooden was more than simply a basketball coach. He was also a teacher of how to be successful in life. read more

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Description:Smart Array 5i Plus with 64MB Battery Backed Write Cache (BBWC) for DL380R G2Part Number(s)
Option Part# 264030-B21
264030-B21 = 274402-001 (board)+ 260740-001 (battery) + 260741-001 (64MB Cache)
Overview:The new Smart Array 5i Plus Controller is an intelligent array controller for entry-level hardware-based fault tolerance for protection of OS applications and logs. Dual Ultra3 SCSI channels (one internal and one external) allow you to support all Ultra2 and/or Ultra3 SCSI internal hard disk drives as well as support up to 14 Ultra2 and/or Ultra3 SCSI external hard disk drives using the StorageWorks Enclosure 4300 Family by Compaq. The Smart Array 5i Plus controller provides one of the most cost effective alternatives to software-based RAID in the market today. Designed as an optional upgrade with the Compaq ProLiant ML370 Generation (G2) and the ProLiant DL380 Generation (G2) servers the Smart Array 5i Plus controller and BBWC Enabler bundle provides increased performance and worry-free transportable battery backed write cache data protection for all server internal storage needs without consuming a PCI slot.
Part Number264030-B21
Products ID12009
Controller TypeSCSI Controller
SCSI InterfaceUltra2 SCSI, Ultra160 SCSI, Ultra320
Recommended Machine TypeServer
PlatformPC, Unix
Bus TypePCI
Data Transfer Rate320MB/s
Supported RAID LevelRAID 0 RAID 1 RAID 0+1 RAID 5
Buffer Size64MB
Number of Internal Connectors1
Number of External Connectors1
Maximum Supported Devices32


text:
The Community of Disc Golfers and About All Things Disc Golf
Night Flight Doubles
This year we plan to have less night events but more payout! We are hoping to hold one large event each month.
NEXT EVENT: FRI JAN 21st
Join OGDC as we continue the third season of Night Flight Doubles at Bill Frederick Park at Turkey Lake. Format is random draw doubles with cash payout. Sign-up by 6:15 pm with tee-off at 6:30.
The park typically closes at 5 pm during the fall and winter months. If you are not in the park by official closing time, ODGC will grant you entry for the standard $2 park entrance fee before 6:15pm. Please call James LeBaube at 704-609-1296 for access into the park. The club will send someone to the front gate.
$15 Entry ($14 for payout/CTP's $1 for operating supplies)
$2 Park entrance fee (if arriving after normal park hours, ODGC will collect)
$1 Ace Fund – optional
Glow sticks and players supplies available for purchase
Views: 47
© 2015 Created by Terry "the Pirate" Calhoun.


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Keyshia Cole 'Woman to Woman' Tracklisting: Meek Mill, Ashanti, Robin Thicke Join
Her Woman to Woman album cover may have startled us a bit, but Keyshia Cole's tracklisting for the effort doesn't disappoint. The LP features 12 tracks with collaborations from five special guests.
Crooner Robin Thicke shows up for Cole's fifth studio effort on "Next Move"


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At Toad Hall, a 32-acre property composed of five log cabins located in Tennessee's Great Smoky Mountains, interior decorator Suzanne Kasler designed polished interiors set against a rustic backdrop of massive logs and boulders. The walls and ceiling of the guest bedroom, pictured, inspired its furnishings, including the soft, neutral bedding and cream-colored hangings of the hickory-log four-poster. Toad Hall "is a combination of refined and rugged," remarks the owner. "From blue jeans to ball gowns, it works for everything." (June 2009)
Photo: Jeff Herr


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Specific Cancers
- Adrenal Cancer
- Bile Duct Cancer
- Bladder Cancer
- Bone Cancer
- Brain and Central Nervous Cancer
Breast Cancer
- Advanced Reading
- Cancer FAQs
- Deciding on Treatment
- Managing Side Effects
- Overview
- Prevention and Screening
- Understanding Your Diagnosis
- Carcinoma of Unknown Primary
- Cervical Cancer
- Colorectal Cancer
- Endometrial Cancer
- Esophageal Cancer
- Ewing Sarcoma
- Eye Cancer
- Gallbladder Cancer
- Head and Neck Cancer
- Hodgkin Disease
- Kaposi's Sarcoma
- Kidney Cancer
- Laryngeal Cancer
- Leukemia - Acute Lymphocytic Leukemia (ALL)
- Leukemia - Acute Myelocytic (AML)
- Leukemia - Chronic Lymphocytic Leukemia (CLL)
- Leukemia - Chronic Myelogenous Leukemia (CML)
- Leukemia - General
- Liver Cancer
- Lung Cancer
- Malignant Mesothelioma
- Multiple Myeloma
- Non-Hodgkin Lymphoma
- Oral Cancer
- Other Cancers
- Ovarian Cancer
- Pancreatic Cancer
- Penile Cancer
- Pituitary Cancer
- Prostate Cancer
- Skin Cancer - Melanoma
- Skin Cancer - Non-Melanoma
- Soft Tissue Sarcoma
- Stomach Cancer
- Testicular Cancer
- Thymus Cancer
- Thyroid Cancer
- Urethral Cancer
- Uterine Cancer
- Vaginal Cancer
- Vulvar Cancer
How to Tell Your Healthcare Team How You Feel When You Have Kidney Cancer
Treating your cancer to get the best results is important. But your quality of life also matters. Let your doctor and nurse know if you are experiencing any side effects or discomfort. Make sure to tell your doctor or nurse how these problems affect your day-to-day life. Your healthcare team is there to help you manage your symptoms as well as to treat your cancer.
It's normal to worry about any problems you may have. You may ask yourself, "Is the cancer getting worse? Are the treatments working?" Talk with your doctor and nurse about your concerns. You also need to let them know as much about the problem as possible. Keep a record of the following information and take it to your appointments.
What the problem is. Describe the problem (diarrhea, depression, appetite loss) that you're concerned about. Be as specific as possible.
Where the problem is. Is there a specific area that is affected, such as your stomach or your head?
When it started. How long have you had the problem? Did you first notice the problem before or after a treatment session? Did you have it before you started treatment? Is it a constant problem? Or do you notice that it's worse or better at certain times? Does it come and go?
How bad it is. If you had to rank the problem on a scale from 0 to 10 (0=not bad, 10=worst), where would it rank?
Triggers that make the problem better or worse. Are there certain activities or environments that affect your symptoms?
How the problem affects your day-to-day life. Have you had to stop any activities because of the problem? Has your life changed because of the problem?
What you're currently doing to manage the symptoms. Is it helping? Be sure to share any complementary or alternative therapies that you may be trying at home.
It might help you to keep a chart of your symptoms. Yours might look something like this.
Sample Side Effects Tracking Chart
Intensity Rating
Severity Rating
(What did you do? Did it work?)
Jan 3
Tried to watch a funny video but couldn't pay attention. Put a cool wet towel on my forehead and lay down in a dark room. After a nap, I felt better.


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At age 79, Willie Nelson has just signed a major deal with Sony Records, released the new album, Heroes, with rapper Snoop Dogg and covers of Coldplay, and agreed to perform at Wednesday's CMT Awards with an all-star back up group of Nashville's emerging country singers.
On Tuesday, on the way to perform with the Nashville Symphony, Nelson made a pit stop at the Cannery Ballroom to address an audience of country music executives, most decades his junior, about making it in the music business as part of a two-day Country Music Summit business meeting.
In his younger days, the aspiring singer-songwriter spent a year on a Nashville hog farm.
"I'd been told all along this was the place to go," Nelson said. "This is where music folks are, and if you had something to sell these folks here might buy it. It sounds kind commercial but that's really the way it was to me back then. Because I really needed some help. I was from Texas. I needed to branch out a little bit. So I moved here to give it a try."
As a songwriter he managed to enjoy some early success, writing hits for other performers, songs such as "Funny How Time Slips Away," performed by Billy Walker, and "Crazy," Patsy Cline's hit.
He sang at the Grand Ole Opry.
But he ended up moving back home to Texas to kickstart his career as a performer, launching outdoor festivals in the months after Woodstock and inviting Nashville friends such as Waylon Jennings to perform in concerts that attracted tens of thousands of fans.
The performances eventually earned Nelson, Jennings and others influenced by folk and rock music — and the culture of the 60s — the nickname "outlaw country." It was a label that Nelson said, "we knew we weren't," but nevertheless proved to be a stroke of marketing genius.
"It was the best sales idea anybody could come up with," Nelson said. "It was genius. Someone else wrote that ladies love outlaws. That was good, too."
Since then, Nelson has gone on to sell more than 50 million albums. He's known for iconic American songs such as "Mama Don't Let Your Babies Grow up to Be Cowboys" and "Always on My Mind," a hit originally sung by Elvis Presley.
In February, Nelson announced he was returning to Sony Records, a label he had left in 1993. Nelson worked for several other labels in the intervening years, including Island, Lost Highway and Rounder.
At Tuesday's conference, Nelson didn't spend much time discussing his decision to return to a major record label in an era when many stars and fresh talent choose small, independent labels. Tim McGraw and Martina McBride are signed with Nashville's Big Machine Label Group, for example. Headliner Jason Aldean is signed with the independent label Broken Bow.
But singer-songwriter Dwight Yoakam, whose talk preceded Nelson's at the Country Music Summit, explained the allure of the big labels to already-famous artists.
"Art, generally, is a medium that delivery of depends on collaboration," Yoakam said, explaining his decision to return to Warner Music Group, a label he left more than a decade ago. for deals with independent labels. He came back to Warner last summer.
"You have to have somebody who takes what you do to the people. It's tough to handle out of the back of a car or a pick-up truck. There's still an international network that somebody like Warner Music Group can use, a focused mechanism that the four majors (labels) bring to the table. That distribution chain is still there."


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For example if I use WinRAR to encrypt a file and put a password on the archive how secure is it? I keep a personal journal and am thinking of doing this, or is there a better way? It's just one huge .docx file.
Out of curiosity, can what someone writes in their journal be used to incriminate someone in court?


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Terms & Conditions.
Some product exclusions may apply. See product page details for more information. Clearance items are final sale. Price matching does not apply to this or any promotion. Offer is valid until September 13 at 11:59 PM PST. May not be combined with any other offer/discount (unless specified), including invitation credits, promotional gift cards and/or special discount links. Discount applied upon checkout. Log in to your account to see your savings.
The sly bird on the Owl cover strikes a balance between cool looks and warm natural materials. Crafted from real ash wood, this laser-cut cover will add the subtle singular style your iPad Mini needs to help it become as good looking as you already are.
Made for the iPad Mini. Full access to all ports and buttons. Laser cut from real wood. Cover has a simple peel-and-stick backing for easy placement. To apply, simply peel off the backing paper and stick the cover on your device.
This product has no reviews yet. Be the first to review this product. Write a review
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|All Content
|View all podcasts & RSS feeds
Originally published on Mon December 16, 2013 6:46 am
Weeks of post-election political limbo have ended in Germany. The country's main center-left party has voted to join the coalition government of Angela Merkel. The move clears the way for her to start her third term as chancellor.


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Ecommerce Software
CORESense's best practices expertise and cloud-based ecommerce software let's you sell online with minimal hassle and maximum profitability.
Retail Management Software
Cloud-based multi-store/multi-channel retail management software streamline operations, connect with customers and grow sales in-store and online.
Professional Services
Full business review and analysis, data model design, data collection and import assistance, testing and training enabling you to make optimal use of CORESense
|CORESense Monthly Product Demonstration
|Join us for the demonstration on Wednesday, September 16th at 2PM Eastern
|Space is limited.
|Reserve your seat now at: https://coresense.webex.com/coresense/onstage/g.php?d=924646894&t=a
Read this case study to learn how this multichannel channel shoe retailer maintains its competitive advantage with the help of technology provided by CORESense.


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Bridal Gowns by AF Couture® presents luxurious beading and embroidery that accent the sophisticated silhouettes and fit of this passionate Collection.
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For this stunning wedding dress and gown collection, we aim to bring you luxurious, contemporary designer wedding dresses with truly universal appeal. Knowing that it's really the personal touches that make you shimmer, we crown each wedding gown with incredible fine detailing and expertly crafted embellishments. Once again hitting the "on trend" mark, these award-winning designer dresses are a testimony to our belief that every woman deserves to feel like a princess on her special day.
Mori Lee Wedding Dresses
Wedding Dress 2771
Embroidered Lace Appliques with Crystal Beading on Soft Tulle
Wedding Dress 2772
Embroidered Lace Appliques on Chantilly Lace with Crystal Beading and Soft Net with Lace Ruffles
Wedding Dress 2773
Lace and Embroidered Appliques on Net with a Scalloped Hemline
Wedding Dress 2774
Crystal Beading Trims the Net Gown Decorated with Embroidered Lace Appliques
Wedding Dress 2775
Diamante and Crystal Beading Decorates the Alencon Lace Bodice Onto the Soft Net Skirt
Wedding Dress 2776
Allover Alencon Lace Gown with Delicate Crystal Beading and Scalloped Hemline
Wedding Dress 2777
Elegant Venice Lace Bodice onto the Classic Tulle Ball Gown
Wedding Dress 2778
Chantilly Lace Gown Decorated with Venice Lace Appliques and Scalloped Hemline
Wedding Dress 2779
Crystal Beaded, Alencon Lace Appliques on Net Over Chantilly Lace with Scalloped Hemline Lace
Wedding Dress 2780
Alencon Lace and Embroidered Appliques Over Chantilly Lace with Delicate Beading and Soft Net Skirt
Wedding Dress 2781
Majestic Embroidered Appliques Onto Net
Wedding Dress 2782
Alencon Lace on Net Over Soft Satin with Crystal Beading
Wedding Dress 2783
Alencon Lace Appliques on Net with Wide Scalloped Hemline Lace
Wedding Dress 2784
Embroidered Appliques Create an Elegant, Allover Pattern Trimmed with Crystal Beading and Scalloped Hemline
Wedding Dress 2785
Alencon Lace Appliques on Net with Crystal Beading and Scalloped Hemline Over Soft Satin
Wedding Dress 2786
Diamante and Crystal Beaded Neckline on the Net Gown Decorated with Venice and Alencon Lace Appliques and Scalloped Hemline
Wedding Dress 2787
Delicate Beading Onto the Patterned Alencon Lace on the Tulle Ball Gown with Wide Hemline Border
Wedding Dress 2788
Crystal Beaded Embroidery Cascades onto the Net Gown Over Soft Satin with Scalloped Hemline
Wedding Dress 2789
Crystal Beaded Bodice Meets the Cascading Alencon Lace Appliques on Net Over Soft Satin
Wedding Dress 2790
Diamante and Swarovski Crystal Beaded Embroidery Cascades Over the Net Gown
Wedding Dress 2791
Crystal and Diamante Beading Decorates the Intricate Embroidery on the Tulle Ball Gown
Wedding Dress 2701
Venice Lace Appliques on Net with Diamante Beaded Trim
Wedding Dress 2702
Embroidered Appliques on Net Over Chantilly Lace with Crystal Beading- Available in Three Lengths: 55 inches, 58 inches, 61 inches
Wedding Dress 2703
Diamante Beaded Embroidery on Duchess Satin
Wedding Dress 2704
Elegant Alencon Lace with Crystal Beaded Straps- Available in Three Lengths: 55 inches, 58 inches, 61 inches
Wedding Dress 2705
Calais Lace Appliques on Net Edged with Crystal Beading
Wedding Dress 2706
Venice Lace Appliques on Net with Detailed Diamante Beading
Wedding Dress 2707
Chantilly and Alencon Lace Appliques on Net with Crystal Beading
Wedding Dress 2708
Alencon Lace on Net with Crystal Beading- Available in Three Lengths: 55 inches, 58 inches, 61 inches
Wedding Dress 2709
Crystal Beaded Embroidery and Alencon Lace Appliques on Net- Available in Three Lengths: 55 inches, 58 inches, 61 inches
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- 2
- people in the U.S. have this name View Dean Osterdyk on Whitepages
Meaning & Origins
Transferred use of the surname, which has a double origin. In part it is a local name for someone who lived in a valley (Middle English dene, Old English denu) and in part an occupational name for someone who served as a dean, i.e. ecclesiastical supervisor (Latin decanus). The given name also sometimes represents Italian Dino (a short form of names such as Bernardino), as in the case of the American actor and singer Dean Martin (1917–95).
|319th in the U.S.
The meaning of this name is unavailable
|177,834th in the U.S.
Nicknames & variations
Deanna, Deana, Deanne, Deann, Deane, Deanie, Deano, Deannie, Deania, Deani
Oster, Osteen, Osterman, Osterhout, Ostendorf, Osterberg, Ostermann, Osterhoudt, Ostertag, Osten


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Big Blue Media Tower
All-in-one music and TV sound system
One sleek media tower. All your entertainment.
- Stream music wirelessly from any Bluetooth-enabled smartphones, tablets, computers and more
- Connect to TV via optical or RCA cables (included) to boost the audio of your favorite shows, movies, and sports
- Two 20-watt, full-range stereo speakers are offset for room-filling stereo sound
- 30-watt powered subwoofer is ported for deep, omnidirectional bass
- RCA inputs and Aux Out
- Measures 38 1/8" x 8" x 8"
Hear details you've never heard before–no matter where you sit. Big Blue Media Tower has two full-range stereo speakers (each with a mid and tweeter) set at different angles. They project sound out and away from each other to reflect off walls and the ceiling, filling every inch of the room with huge, three-dimensional sound. Media Tower also has a powered subwoofer, ported to extend low frequency sound for deeper, richer omnidirectional bass.
No docks. No limits.
Stream all your favorite audio from Big Blue Media Tower by connecting wirelessly with any Bluetooth-enabled device, from your smartphone or MP3 player to your laptop or tablet. Pairing is easy–just press the Big Blue button on the top of media tower and select Big Blue Media Tower from your device's Bluetooth® settings menu. To reconnect the same device later, just press the Big Blue button on your tower and start listening–from up to 30 feet.
Two ways to the get the same amazing sound. You can stream your audio wirelessly, or use the included optical or RCA cables to connect Big Blue Media Tower to your TV. Give your favorite shows, sports and games an instant audio upgrade without having to place your TV above a traditional sound bar. Media Tower comes with RCA inputs an AUX Out port, and measures 38 1/8" x 8" x 8".
Get bigger sound for all your devices. Order your Big Blue Media Tower from Brookstone today.
Big Blue Media Tower
Big Blue Media Tower
|Power Supply
|AC 100V-240V ~ 50/60Hz (Input), DC 18V/3.5A (Output)
|Total Power Output
|50 Watts
|Class 2, up to 33 ft (10 m)
|Bluetooth 2.1 with A2DP and AVRCP
|Frequency Range
|45Hz - 20kHz
|2 x 1.5" Tweeter + 2 x 3" Mid-range driver + 1 x 4" Subwoofer
|Product Weight
|22 lbs. (approx)
|Product Dimensions
|7.9"l x 7.9"w x 38.2"h
Big Blue Media Tower
Brookstone-branded products are backed by a one-year limited warranty. Some massage chairs are backed by a two-year limited warranty (see massage chair product pages for details).
Our innovative products will provide you and your family with years of enjoyment. And with the Brookstone Protection Plus service and replacement plans, you'll have the added peace of mind knowing that if the unexpected does happen, we will simply repair or replace your covered product. Our enhanced coverage goes above and beyond the manufacturer's warranty, saving you time and money.
- 2-Year Extended Replacement Plan $19.99
- 3-Year Extended Replacement Plan $29.99
804336p_manual 804336p_manual
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Bubble Club Side Table by Kartell
designed by Philippe Starck
Made of batch-dyed polyethylene, this industrial side table is a groundbreaking piece of modern furniture and modern design in general. Bubble Club is a weather-resistant table and is ideal for porches and patios.
Smart Furniture is an authorized retailer of Kartell furniture and carries the entire Bubble Club family.
Also In This Collection
Materials & MeasurementsMaterials: Smooth batch-dyed polyethylene
Overall: 16.2" h x 20" w x 20" d
Weight: 20 lbs.
Your FurnitureGenius Recommendations
About The Manufacturer
Creativity and technology, glamour and functionality, quality and innovation: a combination of extraordinary factors together with a strategic vision of distribution has contributed to the lasting success of Kartell. By cultural and entrepreneurial choice, since its origins in 1949 the Italian manufacturer has chosen to characterize itself as the market leader in the production of designers articles which use technology and plastic materials. This choice requires a great understanding of materials and transformation technology. A leader in furniture design, Kartell addresses an international audience with a collection unique in originality, variety, size, and range.
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DesignerFew designers have had an impact across the world of design like Philippe Starck. From modern furniture, restaurant interiors, to architecture, the French icon has made lasting impressions to all those exposed to his work. However, his most influential designs have been in the world of staggeringly original mass produced consumer goods. Renowned for his collaboration with the Italian design and manufacturing firm Kartell, Starck's most famous works include the Ghost Chair series and the Bubble Club group. Despite all his past achievements, he is still highly active in the world of modern design.
Shipping & Delivery
The Kartell Bubble Club Side Table usually arrives within 3-6 business days from the date of purchase.
A shipping fee equivalent to 3 percent of merchandise value applies to Kartell furniture.
For additional information, please see our shipping and delivery policies page.
To receive the most competitive shipping rates available on this Kartell item, call our sales team at 888-467-6278!
Product Q&A
Q: Can the Bubble Club Side Table be used outside?
A: Yes, it is suitable for outside use.
The Bubble Club Side Table by Kartell comes with a 1-year warranty.


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"Desk Caddy"
By Harold G.
Comments: I keep a lot of different pens and markers on my desk and needed something larger than a pencil cup for my desk. This caddy has three large compartments and holds all my stuff, and it looks great on my desk. Very pleased.


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Wedding Dresses near prairieville,la
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This band's really gone to the dogs (Created August 9, 2013)
Meet Houndmouth, the name derived from a neighbor's barking dog.
No, seriously.
This four-piece band featuring Katie Toupin, Matt Myers, Zak Appleby, and Shane Cody were having a late night recording session in hopes of eliminating the neighborhood's ambient sounds. Instead, sirens and car horns were replaced by dogs barking in the background. It was Shane who ultimately suggested the recordings needed to be scrapped because they "contained too much houndmouth." Just like that, the band was christened.
Formed in late 2011 in Southern Indiana, Houndmouth had just a four-song EP before releasing their first full-length album in 2013. Of course "From The Hills Below The City" showcases their folk-rock/American-y sound that also hits the very raw and real threshold the band strives to reach. From Katie's vocals on "Houston Train" to the country-gospel harmonies on "Krampus," the band is well on its way to becoming an established household name.
Houndmouth recently toured the globe with the likes of Grace Potter, Alabama Shakes, Drive-By Truckers and more. They also made their national television debut on "Late Night with Conan O'Brien" and have confirmed a 30-date headlining U.S. fall tour, including dates with Jason Isbell and Court Yard Hounds. The trek includes two-night stands in New York City (November 15-16) and Chicago (November 22-23, as well as performances at Austin City Limits, the Newport Folk Festival, Lollapalooza, the Americana Music Festival, and more.
The awesome thing about Houndmouth? They all sing, whether its lead or harmony, and each of them take turns as frontman or woman. The cohesion of a youthful, excited group on stage has swelled crowds that will only continue to grow with this ambitious group of twentysomethings. You can take that to the bank, straight from the Houndmouth.
Drive-By Truckers Announce Fall Tour Dates
Patterson Hood and the rest of Drive-By Truckers dropped English Oceans back in March and are wrapping up their summer tour next weekend. Never a band that rests for long, DBT has announced 14 more tour dates for this October with the support of Houndmouth and St. Paul and The Broken Bones at select locations.…Read more
Houndmouth Ticket PricesFind Tickets
Past 12 Months
$229Average Price
$1,559High price
$15Low price
Tour Dates
date city state venue 2/20/2015 Madison WI High Noon Saloon 2/21/2015 Saint Paul MN Turf Club 2/23/2015 Iowa City IA Blue Moose Tap House 2/24/2015 Omaha NE The Waiting Room 2/26/2015 Boulder CO Fox Theatre 2/27/2015 Aspen CO Belly Up Aspen 2/28/2015 Denver CO Bluebird Theater 3/2/2015 Lawrence KS The Bottleneck 3/3/2015 Columbia MO Rose Music Hall 3/5/2015 Bloomington IN Bluebird Nightclub 3/6/2015 Knoxville TN Bijou Theatre 3/7/2015 Atlanta GA Terminal West 3/8/2015 Nashville TN 3rd & Lindsley 3/26/2015 Louisville KY Brown Theatre 3/28/2015 Pittsburgh PA Mr. Smalls Recording Studio 3/31/2015 Brooklyn NY Rough Trade NYC 4/1/2015 New York NY Bowery Ballroom 4/3/2015 Cambridge MA The Sinclair 4/4/2015 Philadelphia PA Union Transfer 4/9/2015 Toronto ON Lee's Palace 4/10/2015 Detroit MI Saint Andrews Hall 4/11/2015 Grand Rapids MI The Intersection 4/15/2015 Hamburg HH Molotow 4/26/2015 Washington DC 9:30 Club 4/29/2015 Charlottesville VA Jefferson Theater 4/30/2015 Carrboro NC Cat's Cradle 5/8/2015 Cleveland OH Beachland Ballroom 5/9/2015 Columbus OH Newport Music Hall 5/15/2015 Gulf Shores AL Gulf Shores Beach 5/28/2015 Indianapolis IN The Vogue 5/29/2015 St Louis MO The Ready Room 5/30/2015 Kansas City MO The Record Bar 6/2/2015 Little Rock AR Revolution Music Room 6/4/2015 Morrison CO Red Rocks Amphitheatre 6/5/2015 Austin TX Scoot Inn 6/9/2015 Jackson MS Duling Hall 6/10/2015 Birmingham AL Saturn 6/11/2015 Manchester TN Manchester Farm 6/11/2015 Manchester TN Manchester Farm 6/20/2015 Portland OR Wonder Ballroom 6/21/2015 Seattle WA Neumos 6/23/2015 San Francisco CA Great American Music Hall 6/25/2015 West Hollywood CA The Troubadour 6/27/2015 Santa Ana CA Constellation Room 7/11/2015 Chicago IL Grant Park 7/17/2015 Louisville KY Louisville Waterfront Park 7/17/2015 Louisville KY Louisville Waterfront Park
date city state venue 2/6/2014 Bloomington IN Bluebird Nightclub 2/7/2014 Chicago IL Lincoln Hall 2/8/2014 Evanston IL SPACE 2/9/2014 Milwaukee WI Turner Hall Ballroom 2/11/2014 Omaha NE The Waiting Room 2/12/2014 Lawrence KS The Bottleneck 2/14/2014 Denver CO Larimer Lounge 2/15/2014 Denver CO Larimer Lounge 2/18/2014 Norman OK Opolis 2/19/2014 Austin TX The Parish 2/20/2014 Dallas TX Sons of Hermann Hall 2/21/2014 Little Rock AR Revolution Music Room 3/6/2014 Charlotte NC Visulite Theatre 3/7/2014 Saxapahaw NC Haw River Ballroom 3/8/2014 Washington DC The Hamilton 3/11/2014 Portland ME Port City Music Hall 3/12/2014 South Burlington VT Higher Ground 3/13/2014 Providence RI Columbus Theatre 3/14/2014 New York NY Bowery Ballroom 3/15/2014 Brooklyn NY Music Hall of Williamsburg 3/18/2014 Ithaca NY The Haunt 3/19/2014 Toronto ON Horseshoe Tavern 3/21/2014 Newport KY The Southgate House Revival 3/22/2014 Knoxville TN Bijou Theatre 3/23/2014 Nashville TN The Stone Fox 5/1/2014 Florence AL 116 5/3/2014 Mobile AL Callaghan's Irish Social Club 5/5/2014 Orlando FL The Social 5/8/2014 Charleston SC The Pour House 5/9/2014 Atlanta GA Atlantic Station 5/10/2014 Atlanta GA Atlantic Station 5/23/2014 George WA Gorge Amphitheatre 5/25/2014 New Albany IN The Grand New Albany 6/6/2014 Chattanooga TN Nightfall Concert Series 6/7/2014 Asheville NC The Orange Peel 6/13/2014 Snowmass Village CO Snowmass Town Park 6/20/2014 Richmond VA Brown's Island 6/21/2014 Brooklyn NY Brooklyn Bowl 6/23/2014 South Burlington VT Higher Ground 7/18/2014 Cincinnati OH Sawyer Point 7/19/2014 Cincinnati OH Sawyer Point 8/14/2014 Morrison CO Red Rocks Amphitheatre 8/16/2014 Cockeysville MD Oregon Ridge Park 9/18/2014 Lexington KY Kentucky Theatre 9/23/2014 Phoenix AZ Rhythm Room 9/25/2014 Solana Beach CA Belly Up Tavern 9/27/2014 San Francisco CA The Independent 10/23/2014 Columbus OH Newport Music Hall 10/24/2014 Chicago IL Riviera Theatre 10/25/2014 Springfield MO Gillioz Theatre 10/29/2014 Oxford MS The Lyric Oxford 10/30/2014 Nashville TN Ryman Auditorium 11/1/2014 Charlotte NC The Fillmore Charlotte
date city state venue 1/24/2013 Tulsa OK Cain's Ballroom 2/2/2013 Baltimore MD Rams Head Live 2/4/2013 Baltimore MD Rams Head Live 2/14/2013 Oullins B9 Le Clacson 2/21/2013 London Hck The Shacklewell Arms 2/24/2013 Rotterdam South Holland Rotown 3/12/2013 Austin TX The Blackheart 4/5/2013 Arlington VA IOTA Club & Cafe 4/6/2013 Philadelphia PA World Cafe Live 4/10/2013 New York NY Mercury Lounge 4/16/2013 Toronto ON Horseshoe Tavern 4/17/2013 Pontiac MI The Crofoot-Pike Room 4/18/2013 Chicago IL The Hideout 4/26/2013 Louisville KY Iroquois Amphitheater 4/27/2013 Lexington KY Cosmic Charlie's 5/16/2013 Brighton Bnh Brighton Dome 5/21/2013 Lille La Peniche 6/3/2013 London London Rough Trade East 6/6/2013 Los Angeles CA Drai'S Hollywood 6/21/2013 Ithaca NY The Haunt 6/22/2013 Brooklyn NY Brooklyn Bowl 6/22/2013 Brooklyn NY Brooklyn Bowl 6/25/2013 Allston MA Great Scott 6/26/2013 Burlington VT Signal Kitchen 6/27/2013 Montreal QC Divan Orange 6/28/2013 Toronto ON The Garrison 6/29/2013 Cleveland OH Beachland Tavern 7/13/2013 Louisville KY Belle of Louisville 7/26/2013 Newport RI Fort Adams State Park 7/27/2013 Newport RI Fort Adams State Park 7/30/2013 Hoboken NJ Maxwells 7/31/2013 Pittsburgh PA Brillobox 8/1/2013 Indianapolis IN The Rathskeller 8/2/2013 Chicago IL Grant Park 8/2/2013 Chicago IL Grant Park 8/8/2013 San Francisco CA California Academy of Sciences 8/9/2013 San Francisco CA Golden Gate Park 8/30/2013 Salisbury Dor Larmer Tree Gardens 9/5/2013 Manchester Lancashire The Castle Hotel 9/7/2013 Liverpool Msy The Shipping Forecast 9/9/2013 Brighton East Sussex The Hope 9/16/2013 Berlin BE Berghain 9/18/2013 Paris Cafe de la Danse 9/21/2013 Nashville TN Mercy Lounge 9/27/2013 Atlanta GA The Earl 9/29/2013 Birmingham AL Workplay Theatre 10/1/2013 New Orleans LA Gasa Gasa 10/5/2013 Austin TX Red 7 10/8/2013 Tucson AZ Club Congress 10/14/2013 Vancouver BC The Media Club 10/17/2013 Fargo ND The Aquarium 10/18/2013 Fargo ND The Aquarium 10/19/2013 Madison WI High Noon Saloon 11/8/2013 Nashville TN Mercy Lounge 11/9/2013 Lexington KY Busters Billiards and Backroom 11/10/2013 Asheville NC The Grey Eagle 11/12/2013 Charlottesville VA The Southern Cafe and Music Hall 11/13/2013 Philadelphia PA World Cafe Live 11/14/2013 Boston MA Royale Boston 11/15/2013 New York NY Mercury Lounge 11/16/2013 New York NY Mercury Lounge 11/19/2013 Columbus OH A and R Music Bar 11/21/2013 Indianapolis IN The Vogue 11/22/2013 Chicago IL Schubas Tavern 11/23/2013 Chicago IL Schubas Tavern 11/29/2013 Louisville KY Headliners Music Hall 11/30/2013 Louisville KY Headliners Music Hall 12/31/2013 Chattanooga TN Center Park
date city state venue 9/14/2012 Indianapolis IN Radio Radio 9/21/2012 Hamden CT The Space 10/6/2012 West Hollywood CA The Troubadour 10/9/2012 Tucson AZ Club Congress 10/12/2012 New Orleans LA Circle Bar 10/13/2012 Oxford MS Proud Larry's 10/18/2012 Pittsburgh PA The Smiling Moose 11/7/2012 London London Rough Trade East 11/23/2012 Lexington KY Busters Billiards and Backroom 11/24/2012 Louisville KY Headliners Music Hall 12/1/2012 Indianapolis IN Radio Radio


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Korg has teamed up with SoundCloud to offer a worldwide remix contest for KORG Gadget for iPad owners! From Feb 28 through Mar 31 2014, owners can enter the contest by uploading a remixed track to "GadgetCloud". Winners will receive special prizes for their remixes
This remix contest is open to everyone, amateur and professional alike! Gain new skills while remixing tracks in competition with users throughout the world. Enter this remix contest and distribute your work all over the world!
Product Information
KORG Gadget for iPad


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Saturday, January 03, 2004Chattanooga, Tenn. (Jan. 3, 2004) -- Trailing by 27 points at halftime, the East Tennessee State women's basketball team trimmed the deficit down to 14 points in the second half, but eventually fell to defending Southern Conference champion Chattanooga, 72-54, from McKenzie Arena Saturday night. The Lady Bucs could not overcome one of their worst shooting performances of the year. ETSU shot just 24.2 percent from the floor in the first half and just 4-of-10 from the free throw line. Chattanooga, on the otherhand, utilized its home court advantage to out-rebound the Lady Bucs 30-16 in the first stanza and shot 51.4 percent as a team in taking a 48-21 advantage into the locker room at the half. ETSU was led by Jessica Lonon (Sylvania, Ga.), who scored a season-high 14 points and grabbed 11 rebounds, while recording her first double-double of the season. Kiya Verdell (Hartwell, Ga.) pitched in 13 points on 6-of-20 shooting (1-of-6 from 3-pt. range), and Lauren Trantham (Asheville, N.C.) added 12 points. Tiffani Mayes (Sumter, S.C.), ETSU's leading scorer going into the game, was held to just four points and tied Verdell and Trantham with seven rebounds apiece. Verdell, along with freshman guard Brooke Wilhoit (Sevierville, Tenn.) both had four steals to pace the Lady Bucs. ETSU will return home on Monday, Jan. 5, where the Lady Bucs are a perfect 4-0 this season. They will host College of Charleston at 7 p.m. in Memorial Center.
|Copyright ©2015 East Tennessee State Athletics. All Rights Reserved.


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Student Group Reservation Requests for Fall 2015
The Information Desk is conveniently located in the Thomas Commons. We are here to answer questions, look up phone numbers, and supply local and campus information. Stop by anytime or call us at 895-4334.
Office Hours:
- Summer Hours
Monday-Friday 8 a.m. to 5 p.m.
Saturday & Sunday Closed
- When School is in Session:
Monday-Thursday 8 a.m. to 10 p.m.
Friday-Saturday 8 a.m. to midnight
Sunday 10 a.m. to 10 p.m.
- Block Break Hours:
Wednesday- Saturday 8 a.m. to 9 p.m.
Sunday 10 a.m. to 10 p.m.
Thomas Commons Hours:
- Summer Hours
6 a.m. to 7 p.m.
- When School is in Session:
6 a.m. to 12 midnight


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Lewis Hamilton insists he has no regrets about his decision to quit McLaren for Mercedes...
Lewis Hamilton insists he has no regrets about his decision to quit McLaren for Mercedes.
The 27-year-old's long association with the Woking-based squad will come to an end on December 31 after he signed a three-year deal with Ross Brawn's Mercedes outfit.
However, his new employers have endured a nightmare end to the 2012 season as Nico Rosberg and Michael Schumacher claimed just 16 points between them since Hamilton's move was confirmed in September.
With the Brackley squad struggling, McLaren team boss Martin Whitmarsh and several pundits have suggested that he is regretting the decision, but Hamilton is sticking to his guns.
"No, I'm really, really happy about it. You can't live with regrets in your life," he said.
"You have to make a decision and stick with it. Whether it's right or wrong, you just have to make the most of it, stick to your guns and hope you can turn it around or turn it into a positive."
Hamilton, though, admits he will always have a soft spot for McLaren and could return to "his home" one day.
"You can never say never and I've had a great time," said Hamilton. "I think it will always be my home.
"I'll always look at it as where I've come from. Going back there would be nice one day. But I want to go and experience some things for a bit.
"It's almost like leaving home and going travelling for a bit. But I'm doing it with a different company."


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Health Educators
Also called: Clinical Instructor, Health Educator, Health Promotion Specialist, Public Health Educator
In the military: see titles from the Air Force, Army, Coast Guard, or Navy.
In the Air Force: Aerospace Medical Service; Aerospace Medical Service Apprentice; Aerospace Medical Service Apprentice, Neurodiagnostic Medical Technician; Aerospace Medical Service Craftsman, Flight and Operational Medicine Technician; Aerospace Medical Service Craftsman, Independent Duty Medical Technician; Aerospace Medical Service Helper; Aerospace Medical Service Helper, Neurodiagnostic Medical Technician; Aerospace Medical Service Journeyman; Aerospace Medical Service Journeyman, Independent Duty Medical Technician; Aerospace Medical Service Journeyman, Neurodiagnostic Medical Technician
In the Army: Health Care Specialist
In the Coast Guard: SC - Nurse Corps - General
In the Navy: Hospital Corpsman; SC - Nurse Corps - General
- Develop and present health education and promotion programs, such as training workshops, conferences, and school or community presentations.
- Develop and maintain cooperative working relationships with agencies and organizations interested in public health care.
- Develop educational materials and programs for community agencies, local government, and state government.
Education and Training
Arts and Humanities
Basic Skills
Problem Solving
Ideas and Logic
People interested in this work like activities that include helping people, teaching, and talking.
They do well at jobs that need:
You might use software like this on the job:
Web page creation and editing software
Data base user interface and query software
Spreadsheet software
You might like a career in one of these industries:


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Santa Clara has many parks that are maintained by the Parks and Recreation Department for the enjoyment of its residents and visitors. The parks and pools have been brought together in an online storyboard that includes photos, maps and a description of amenities.
Take some time this Thanksgiving holiday to view the information about your local park on the storyboard. The City's parks are a great place to visit after eating dinner with your family and friends.
If you have comments about the new feature, please contact us using the feedback form. We look forward to hearing from you.
City of Santa Clara
The City of Santa Clara is not responsible for the content of external sites.
Thank you for visiting the City of Santa Clara website.


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- Flat Rate Shipping. Any Size Order! -
- A Portion of Proceeds Benefit Baltimore Ravens Athletics -
HOME Baltimore Ravens Gear Baltimore Ravens Mens Baltimore Ravens Mens T-Shirts (320 items) Product ID: 1165343
Main Content
Order as many items as you like with our $6.99 flat rate shipping.
This item ships within two business days.
Order as many items as you like with our $6.99 flat rate shipping.
Need it faster?
If you could put together a fantasy team, you would have to choose the entire Baltimore Ravens lineup. Due to their unbelievable talent on the field, every player on the Ravens is worthy enough of making your fantasy roster. Show off your perfect team with this Fantasy Leader tee. Featuring a distressed "Charm City" slogan and team logo printed on the front, this shirt boasts a massive amount of Baltimore pride!
- 100% Cotton
- Distressed screen print graphics
- Imported
- Officially licensed NFL product
- Tagless collar
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Special event items are produced by manufacturers only after the outcome of a game or event. These are advanced sale items and will ship immediately after they are received in our warehouse.
Manufacturer direct items are shipped directly from the manufacturer. These items are not available for international or expedited shipping.
Customized items can be personalized with options such as your name, your favorite number, and/or designs. Some options may be limited by league rules.


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Brevity is the soul of wit—and also excellent gifts, as it turns out. These lovely adornments from Brevity bear enchanting organic silhouettes and sweet nothings in calligraphic script. The Grid Necklace features an acrylic geometric pendant suspended from a gold-fill chain. Your lady's delight for it will be anything but brief.
The chain is 22" long. The pendant is 1.25"W x 3.25"H.
This product has no reviews yet. Be the first to review this product. Write a review
Enjoy 10% off your first order*


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|West Sacramento, Calif.
|High School:
|River City (Iowa Lakes C.C.)
|Previous College:
|Iowa Lakes C.C.
|Last Team:
As a junior: Played and started in 57 games for the Bobcats…Ranked fourth on the team with a .283 batting average…Was second on the team with 54 hits and 23 RBI…Ranked fourth for the Bobcats in runs (22) and led the team with two triples, while collecting four doubles on the year…Recorded 62 total bases in her first season with Quinnipiac…Posted a .325 slugging percentage and .352 on-base percentage…Collected seven stolen bases.
Before Quinnipiac: Attended Iowa Lakes Community College, where she played softball for two years...In her sophomore year, she held a .482 batting average...She also contributed 81 RBI's, 92 runs, 122 hits, and five homeruns...Placed second in the NJCAA Regionals (D-2) her freshman year...Placed first in the NJCAA Regionals (D-2), her sophomore year, with a 61-11 overall record...Finished in third at the NJCAA Nationals. (D-2)...Was named All-Region in 2009 and 2010...Was selected as an NFCA All-American and a NJCAA All-American in 2009 and 2010...Broke Iowa Lakes Community College records for stolen bases (67), hits (122), and RBI's (81)in a single season...Held a Was a 2008 graduate of River City High School, where she was a four-year letterwinner in softball...Was a three-year captain for River City's varsity softball team...Held a .547 batting average and collected 25 runs at River City in 2007-08...Was named the Most Valuable Player and Offensive Player in 2008...Was selected to the 2008 CVC First-Team...Was named to the All-Conference First Team for three years...Played for the Capital City Comets U-18 Gold team.
Personal: A native of West Sacramento, Cal...Daughter of Antonio Cabrera and Anna Galindo...Has two brothers, Victor and Anthony, and two sisters, Arianna and Valeria...Majoring in psychology.


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I LOVE Pinterest! It's definitely been my inspiration for these 31 Days! If you are not on it, read about why you should be!
Today I thought I would post about some organizational projects I LOVE, but just haven't gotten around to yet, or it just doesn't work with my home.
Have an old VHS tape lying around?
Turn it into a picture + secret storage! (Source)
If you have several milk jugs:
Use them as storage bins for craft supplies, office supplies, etc.! (Source)
Or upcycle it as a lunch box!! (Source)
Ice cube trays…
…as a jewelry organizer! (Source)
Use a plastic rack to organize your freezer:
This is from one of my earlier Take it on Tuesday projects!
For Brown Grocery Bags:
Isn't this super cute? This is a no sew project! (Source)
Check back next week for some more great Pinterest finds!!
© 2011 – 2014, Betsy Pool. All rights reserved.


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Treatment of Behavioral Symptoms: When to Consider Antipsychotic Medications in LBD - Page 4
Antipsychotic Medications
In diseases such as schizophrenia, behavioral symptoms like hallucinations and delusions can be controlled using antipsychotic medications (also called neuroleptics). But in LBD, using antipsychotic medications can be problematic.
We do not know why, but many people with LBD who are treated with antipsychotic medications have very severe reactions. Their cognitive symptoms can become worse and they may appear more sedated. In addition, they may have increased symptoms of parkinsonism. Also, in rare cases, antipsychotic medications may cause a condition called "neuroleptic malignant syndrome" (NMS), which causes severe fever, muscle rigidity and may lead to kidney failure and death. So physicians must be very careful when prescribing antipsychotic medications for someone with LBD.
There are two types of antipsychotic medications: the typical (or traditional) antipsychotics and the newer, antipsychotics.
The typical antipsychotics were the first antipsychotics developed and should NOT be prescribed for people with LBD. Typical antipsychotics include:
- chlorpromazine (Thorazine)
- droperidol (Inapsine)
- fluphenazine (generic only)
- haloperidol (Haldol)
- loxapine (Loxitane)
- molindone (Moban)
- perphenazine (generic only)
- pimozide (Orap)
- thioridazine (generic only)
- thiothixene (Navane)
- trifluoperazine (Stelazine)
The newer or atypical antipsychotics may be helpful for people with LBD if used conservatively. Atypical antipsychotics include:
- aripiprazole (Abilify)
- clozapine (Clozaril)
- fluoxetine & olanzapine (Symbyax)
- iloperidone (Fanapt)
- olanzapine (Zyprexa)
- paliperidone (Invega)
- quetiapine (Seroquel)
- risperidone (Risperdal)
- ziprasidone (Geodon)
Many LBD experts prefer quetiapine or clozapine to control difficult behavioral symptoms.
Clozapine requires frequent blood tests to monitor for the development of potential blood problems. Both quetiepine and clozapine should be given at the lowest dose possible and for the shortest length of time possible to control symptoms.
Some atypical antipsychotic medications (like olanzapine and risperidone) should be avoided if possible, because they have higher incidences of side effects, such as increased parkinsonism, sedation, and orthostatic hypotension. Ask your loved one's physician about possible side effects on motor function before he or she prescribes an atypical antipsychotic medication.
Caregivers should note that several years ago the U.S. Food and Drug Administration (FDA) issued a warning that all antipsychotic medications, both atypical and typical, increase the risk of death in elderly patients with dementia (this includes people with LBD). Doctors and families should discuss the risks associated with antipsychotic medication use, including the side effects of the medication, against the risks of potential physical harm and distress to the patient or caregivers as a result of behavioral symptoms. Caregivers should contact the doctor about any side effects they observe that do not go away within a few days.


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I have a database that has been used for a Virtual Learning Environment (VLE) called Moodle. I needed to do a complete refresh of the system so I backed up the database in PHPMyAdmin into an SQL file.
I dropped all the table off the database (as I have done this before without any problems) and proceeded to reupload the backedup database.
Unfortunately nothing happens!!! I select the SQL file to upload and click Go and the page just sits there for about 5 minutes before it returns a "This Page Cannot Be Displayed" page.
I REALLY need to get this database sorted and I have no idea how to get past this problem. I have tried using the file and copying the data and pasting it in the text box. Nothing works.
Please help!!!!


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Basic Ordering Guidelines
If you are a bookseller and would like to place an order, check the status of an existing order, or receive customer or credit assistance, you can contact us:
By phone
Please call our Customer Care department at (800) 225-5945 or (317) 572-3993. When calling, please have your account number (or SAN), ISBN(s), and invoice or purchase order ready. Representatives are available Weekdays from 8:00 a.m. to 8:00 p.m. EST.
By mail or fax
Orders can be sent to:
John Wiley & Sons, Inc.
Customer Care
10475 Crosspoint Blvd
Indianapolis, IN 46256
Phone: (800) 225-5945 or (317) 572-3993
Fax: (800) 597-3299 or (317) 572-4002
Email: firstname.lastname@example.com
Order Entry Fax: (732) 302-2370 or (732) 302-2300
By EDI (Electronic Data Interchange)
Wiley has multiple options for automating the ordering and accounting processes for higher volume customers. The easiest options are currently Pubnet and Bookseller.Wiley.com.
For larger retail chains and other major customers Wiley also can exchange data using the ANSI X12 format, using either a VAN service or via direct FTP. Wiley currently uses ICCNET as its VAN.
For more information about doing business with Wiley via EDI, please contact your local sales representative. If you have other questions not related to EDI, please visit the Contact Us page.
On the Web
The status of orders placed by any of the above methods can be checked by going to My Account. You can check the status of your outstanding orders and back orders, including carton detail. You can also track the shipping status of your orders with our site's direct links to freight carriers.
For more information please contact the appropriate person from our sales contact list.


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Your photos of the snow
Dec 27, 2012
Thu, 12/27/2012 - 8:25am
Can not view larger images. When the picture is clicked on it just goes blank, what is going on with the web site at the SR?
Thu, 12/27/2012 - 3:41pm
And with a sled....flying down a hill, building a snow man in the front yard...oh, the heck with young. I am going outside and do it anyway. Hi, ho...Frosty, here we come. And maybe a snow angel or two. YIPEEE.
Sun, 12/30/2012 - 12:02am
Curley, I had the same problem. To view the larger image, all you do is click the "Compatibility View" button on the toolbar of your Internet browser window and the picture shows up.


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17 June 2011
Posted in DinoDigs
By: Liz Rosenberg
This book is great for your child to give dad for father's day! It's a perfect blend of tender and funny. Tobias's father is a lot like other fathers - he likes corny jokes, and doing magic tricks, and works really hard at the office. But there the resemblance ends. He has teeth as sharp as steak knives, is forty feet high, and weighs as much as a locomotive. He is, in fact, a tyrannosaurus.
Come check out our DinoDigs exhibit where you can uncover fossils in the dig pit with dad!


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Seeing the Flood Story Through an Ancient Israelite Lens
Pete Shaw highlights the story of Noah to explore how the story would have been understood in ancient times and from there he goes on to explore how we might consider it today.
The Resource Finder has powerful filtering features — check out the tutorial to learn more.
Pete Shaw highlights the story of Noah to explore how the story would have been understood in ancient times and from there he goes on to explore how we might consider it today.
Brian Godawa looks at several aspects of ancient cosmography (descriptions of the universe) that also appear in the Bible, and what these aspects of the text mean for our understanding of Scripture.
"As a Christian and a biblical scholar, I care both about Scripture as truth and about the ongoing scholarly conversation regarding the composition of the Hebrew Scriptures. And so, when I was asked to speak on the story of creation in Genesis 1, I welcomed the opportunity to give my thoughts on the interaction between this text and its ancient Near Eastern context."
The literary conventions employed in Genesis chapter 1 mark it out, not as a scientific document describing material origins, but as a theological polemic against surrounding ancient Near Eastern pagan religions. Creation language here and elsewhere in Scripture is not about establishing scientific origins of material substance and structure but about covenantal establishment and worldview.
Professor Denis Lamoureux presents the theory of evolutionary creation, which claims that the Father, Son, and Holy Spirit created the universe and life through an ordained, sustained, and design-reflecting evolutionary process. The view of origins, says Lamoureux, fully embraces both the religious beliefs of biblical Christianity and the scientific theories of cosmological, geological, and biological evolution.


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Stealth Swivel BoomChair Gaming Chair
Bring gaming, music and movies to the next level!
We have raised the bar on our more traditional styled chairs with a modern off the ground base.
The Stealth Swivel BoomChair™ features reinforced padded armrests and an adjustable headrest for a comfortable entertainment experience. Two 3" 2-way speakers and a 4" sub-woofer provide great sound while you watch your favorite movies, play games or listen to music. Adjust the swivel and tilt to a comfortable position. Also can be powered with 8 D cell batteries (not included).
Dimensions: 28"L x 28"W x 41"H
Weight: 43 lbs.
Stealth Swivel BoomChair Gaming Chair
Stealth Swivel BoomChair Gaming Chair
Our innovative products will provide you and your family with years of enjoyment. And with the Brookstone Protection Plus service and replacement plans, you'll have the added peace of mind knowing that if the unexpected does happen, we will simply repair or replace your covered product. Our enhanced coverage goes above and beyond the manufacturer's warranty, saving you time and money.
Shopping Cart


text:
International Delivery
Their smile will light up a room—and no gift guarantees that smile like our vibrant multicolored roses! Send them a dozen fresh, gorgeous blooms from your Rose Authority. They'll still be smiling when they whisper, "Wow."
Add to their gift:
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|By Marketwired .
Article Rating:
|April 28, 2014 05:04 PM EDT
TORONTO, ONTARIO -- (Marketwired) -- 04/28/14 -- Highvista Gold Inc. (TSX VENTURE: HVV) ("Highvista" or the "Company") announces that it has called a special meeting of shareholders of the Company to consider, and if advisable, to pass a resolution authorizing the board of directors of the Company to implement a share consolidation on the basis of one (1) new share for each ten (10) common shares currently issued or authorized.
The shareholder meeting is scheduled to be held on Wednesday, June 11, 2014 in Toronto. On or about May 15, 2014, the Company intends on mailing a management information circular discussing the business for the meeting to the shareholders of the Company as of May 5, 2014, the record date for the meeting. The Company will also post the circular on SEDAR promptly after mailing.
The proposed consolidation is subject to regulatory approval, including approval of the TSX Venture Exchange, and the board of directors will retain discretion on whether or not to pursue the transaction if shareholder approval is obtained.
In addition, the Company is pleased to provide an update on the status of its secured promissory note with Norvista that is currently in default. Norvista has agreed to forebear any of its rights under the note until June 30, 2014.
About Highvista
Highvista owns 100% of a Mexican subsidiary that controls the 24,055 hectare Canasta Dorada Gold Project. This project is located in the Sonoran Gold Belt immediately adjacent to AuRico Gold's El Chanate Mine. Details of Highvista's Canasta Dorada Project can be viewed at the Company's website at www.highvistagold.com.
Reader Advisory
Certain statements in this press release constitute "forward-looking statements". These forward-looking statements include, but are not limited to, statements regarding the date of the special meeting of shareholders of the Company and mailing and posting to SEDAR of the related management information circular as well as the board of directors' decision whether to complete the proposed transaction described herein. Forward-looking statements express, as at the date of this press release, the Company's plans, estimates, forecasts, projections, expectations, or beliefs as to future events or results. Forward-looking statements are based on certain assumptions, including the key assumptions and parameters on which such estimates are based, involve risks and uncertainties and there can be no assurance that such statements will prove to be accurate. Therefore, actual results and future events could differ materially from those anticipated in such statements. Factors that could cause results or events to differ materially from current expectations expressed or implied by the forward-looking statements, include, but are not limited to, any rescheduling or adjournment of the shareholder meeting and receipt of regulatory and shareholder approvals for the proposed transaction. Readers are cautioned not to place undue reliance on the forward-looking statements contained in this press release. Except as required by law, Highvista assumes no obligation to update or revise any forward-looking statement, whether as a result of new information, future events or any other reason.
Highvista Gold Inc.
Richard Adams
President and CEO
(416) 504-3588
Cloud and datacenter migration innovator AppZero has joined the Microsoft Enterprise Cloud Alliance Program. AppZero is a fast, flexible way to move Windows Server applications from any source machine – physical or virtual – to any destination server, in any cloud or datacenter, using its patented container technology. AppZero's container is also called a Virtual Application Appliance (VAA). To facilitate Microsoft Azure onboarding, AppZero has two purpose-built offerings: AppZero SP for Azure,...
Aug. 30, 2015 11:00 AM EDT Reads: 156
Organizations from small to large are increasingly adopting cloud solutions to deliver essential business services at a much lower cost. According to cyber security experts, the frequency and severity of cyber-attacks are on the rise, causing alarm to businesses and customers across a variety of industries. To defend against exploits like these, a company must adopt a comprehensive security defense strategy that is designed for their business. In 2015, organizations such as United Airlines, Sony...
Aug. 30, 2015 10:30 AM EDT Reads: 425
SYS-CON Events announced today that HPM Networks will exhibit at the 17th International Cloud Expo®, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. For 20 years, HPM Networks has been integrating technology solutions that solve complex business challenges. HPM Networks has designed solutions for both SMB and enterprise customers throughout the San Francisco Bay Area.
Aug. 30, 2015 10:30 AM EDT Reads: 860
Consumer IoT applications provide data about the user that just doesn't exist in traditional PC or mobile web applications. This rich data, or "context," enables the highly personalized consumer experiences that characterize many consumer IoT apps. This same data is also providing brands with unprecedented insight into how their connected products are being used, while, at the same time, powering highly targeted engagement and marketing opportunities. In his session at @ThingsExpo, Nathan Trel...
Aug. 30, 2015 10:15 AM EDT Reads: 208
Red Hat is investing in Tesora, the number one contributor to OpenStack Trove Database as a Service (DBaaS) also ranked among the top 20 companies contributing to OpenStack overall. Tesora, the company bringing OpenStack Trove Database as a Service (DBaaS) to the enterprise, has announced that Red Hat and others have invested in the company as a part of Tesora's latest funding round. The funding agreement expands on the ongoing collaboration between Tesora and Red Hat, which dates back to Febr...
Aug. 30, 2015 10:00 AM EDT Reads: 327
IBM's Blue Box Cloud, powered by OpenStack, is now available in any of IBM's globally integrated cloud data centers running SoftLayer infrastructure. Less than 90 days after its acquisition of Blue Box, IBM has integrated its Blue Box Cloud Dedicated private-cloud-as-a-service into its broader portfolio of OpenStack® based solutions. The announcement, made today at the OpenStack Silicon Valley event, further highlights IBM's continued support to deliver OpenStack solutions across all cloud depl...
Aug. 30, 2015 10:00 AM EDT Reads: 214
Everyone talks about continuous integration and continuous delivery but those are just two ends of the pipeline. In the middle of DevOps is continuous testing (CT), and many organizations are struggling to implement continuous testing effectively. After all, without continuous testing there is no delivery. And Lab-As-A-Service (LaaS) enhances the CT with dynamic on-demand self-serve test topologies. CT together with LAAS make a powerful combination that perfectly serves complex software developm...
Aug. 30, 2015 09:45 AM EDT Reads: 177
With the proliferation of connected devices underpinning new Internet of Things systems, Brandon Schulz, Director of Luxoft IoT – Retail, will be looking at the transformation of the retail customer experience in brick and mortar stores in his session at @ThingsExpo. Questions he will address include: Will beacons drop to the wayside like QR codes, or be a proximity-based profit driver? How will the customer experience change in stores of all types when everything can be instrumented and a...
Aug. 30, 2015 09:15 AM EDT Reads: 432
Through WebRTC, audio and video communications are being embedded more easily than ever into applications, helping carriers, enterprises and independent software vendors deliver greater functionality to their end users. With today's business world increasingly focused on outcomes, users' growing calls for ease of use, and businesses craving smarter, tighter integration, what's the next step in delivering a richer, more immersive experience? That richer, more fully integrated experience comes ab...
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In today's digital world, change is the one constant. Disruptive innovations like cloud, mobility, social media, and the Internet of Things have reshaped the market and set new standards in customer expectations. To remain competitive, businesses must tap the potential of emerging technologies and markets through the rapid release of new products and services. However, the rigid and siloed structures of traditional IT platforms and processes are slowing them down – resulting in lengthy delivery ...
Aug. 30, 2015 08:45 AM EDT Reads: 565
Culture is the most important ingredient of DevOps. The challenge for most organizations is defining and communicating a vision of beneficial DevOps culture for their organizations, and then facilitating the changes needed to achieve that. Often this comes down to an ability to provide true leadership. As a CIO, are your direct reports IT managers or are they IT leaders? The hard truth is that many IT managers have risen through the ranks based on their technical skills, not their leadership ab...
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WSM International, the pioneer and leader in server migration services, has announced an agreement with WHOA.com, a leader in providing secure public, private and hybrid cloud computing services. Under terms of the agreement, WSM will provide migration services to WHOA.com customers to relocate some or all of their applications, digital assets, and other computing workloads to WHOA.com enterprise-class, secure cloud infrastructure. The migration services include detailed evaluation and planning...
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The Internet of Things (IoT) is about the digitization of physical assets including sensors, devices, machines, gateways, and the network. It creates possibilities for significant value creation and new revenue generating business models via data democratization and ubiquitous analytics across IoT networks. The explosion of data in all forms in IoT requires a more robust and broader lens in order to enable smarter timely actions and better outcomes. Business operations become the key driver of I...
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Puppet Labs has announced the next major update to its flagship product: Puppet Enterprise 2015.2. This release includes new features providing DevOps teams with clarity, simplicity and additional management capabilities, including an all-new user interface, an interactive graph for visualizing infrastructure code, a new unified agent and broader infrastructure support.
Aug. 30, 2015 07:15 AM EDT Reads: 494
A producer of the first smartphones and tablets, presenter Lee M. Williams will talk about how he is now applying his experience in mobile technology to the design and development of the next generation of Environmental and Sustainability Services at ETwater. In his session at @ThingsExpo, Lee Williams, COO of ETwater, will talk about how he is now applying his experience in mobile technology to the design and development of the next generation of Environmental and Sustainability Services at ET...
Aug. 30, 2015 07:00 AM EDT Reads: 116


text:
Ridgway softball lost 5-0 to a Curwensville on a windy Thursday afternoon at Francis S. Grandinetti Elementary School.
Consistent winds of 15-20 mph were blowing out throughout the game and the Lady Tide were able to take advantage of the wind blowing out in the first inning. Sarah Wriglesworth led off the game by reaching on a Ridgway defensive miscue. Taylor Goodman followed with a bloop single to left. After a Curwensville flyout, cleanup hitter Tiffany Carter launched a 3-run home run to center field which gave the Lady Tide a 3-0 lead after the first inning.
[1] http://www.ridgwayrecord.com/sites/default/files/Jill Gaio color.jpg
[2] mailto:firstname.lastname@example.com


text:
Blueberry Oatmeal Recipe
- 1-3/4 cups 2% milk
- 1 cup quick-cooking oats
- 1/8 teaspoon salt
- 1/3 cup packed brown sugar
- 1/2 teaspoon ground cinnamon
- 3/4 cup fresh or frozen unsweetened blueberries, thawed
- In a small saucepan, bring milk to a boil. Stir in oats and salt. Cook over medium heat for 1-2 minutes or until thickened, stirring occasionally. Stir in brown sugar and cinnamon. Divide between two serving bowls; top with blueberries. Yield: 2 servings.
Reviews for Blueberry Oatmeal
Sort By :
"I prepared this recipe this morning and declare it a winner. It is not only easy but delicious. So much better than the purchased flavored oatmeal. The only thing I will do next time is leave out the salt. Those of you who salt everything, may want to leave it in. This oatmeal is good anytime. I buy fresh blueberries in the summer and freeze for winter. I highly recommend this recipe as a Volunteer Field Editor for Taste of Home."
"This recipe is simple and delicious, exactly what I need to make a quick breakfast before work. I am on a low-sodium diet, so I omitted the salt and it was great. No more packaged oatmeal for me!"
"made it this morning with gluten free oats! The blueberries are my favorite part! The little burst of juice and flavor makes it that much better."
"I agree that one should be nice and kind and positive, however, when rating a recipe it is necessary to point out the negative aspects as well as the positive."
"If you can't be positive, don't say anything at all!"


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Some times I get invited to Google+ events and these events get shown in my Google+ calendar. The most of these invitations can be considered spam and I would like to uncircle the sender in such cases, but I can't find the information on who has invited me (looks like this is not always the same person as the event administrator). How to know who was the one to invite me?
Take the 2-minute tour ×
Web Applications Stack Exchange is a question and answer site for power users of web applications. It's 100% free, no registration required.
When I receive an invitation in my Google Calendar (I assume you mean Google Calendar, not Google+ Calendar), the sender of the invitation will appear when I click on the event in question. It is mentioned just below the 'Where' entry.
Unfortunately, I'm not sure if this is also the case with invites received from Google+.


text:
Contact KosherEye
|FAQs to Submit Features
How can my products be considered for feature by KosherEye?
What kosher certification is required for my product to be considered for feature?
How do I submit a product for feature consideration?
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How will I know if my product has been accepted for feature?
Can I expect a "bump" in sales after the KosherEye feature?
Will unused items be returned? Please be aware that we cannot return any items sent for consideration. We donate unusable or extra products to charitable organizations.
KosherEye does not accept financial remuneration for any features!


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Late stage luminescence-based cell-based high throughput dose response assay for inverse agonists of the liver receptor homolog-1 (LRH-1; NR5A2)
Name: Late stage luminescence-based cell-based high throughput dose response assay for inverse agonists of the liver receptor homolog-1 (LRH-1; NR5A2). ..more
BioActive Compounds: 54
Related Experiments
Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC)
Affiliation: The Scripps Research Institute, TSRI
Assay Provider: Patrick Griffin, TSRI
Network: Molecular Library Probe Production Centers Network (MLPCN)
Grant Proposal Number: CA134873
Grant Proposal PI: Patrick Griffin, TSRI
External Assay ID: LRH1_INH_LUMI_1536_3XIC50 MDRUN
Name: Late stage luminescence-based cell-based high throughput dose response assay for inverse agonists of the liver receptor homolog-1 (LRH-1; NR5A2).
The goal of this project is to identify modulators (inverse agonists) of the orphan nuclear receptor LRH-1, which has been implicated in cancer by enhancing proliferation and cell cycle progression and metabolic disorders through its regulation of genes involved cholesterol and bile acid homeostasis.
NR5A2 or Liver receptor homologue-1 (LRH-1) is a member of the NR5A, or Ftz-F1, subfamily V nuclear receptors for which there are four members (1). Murine LRH-1 was originally identified due to its sequence homology to the Drosophila Fushi tarazu factor-1 but orthologs have been subsequently identified in several other species including rat, chicken, horse, zebrafish and human (2-7). LRH-1, and its closest family member steroidogenic factor-1 (SF-1, NR5A1), bind to identical DNA consensus sequences (response elements or REs) and both have the ability to bind phospholipids in their ligand binding domains (LBDs) (8-10). However, LRH-1 and SF-1 are expressed in different tissues and thus are considered likely to have non-overlapping, non-redundant functions. SF-1 expression is confined to steroidogenic tissues and adrenals where it regulates development, differentiation, steroidogenesis and sexual determination (5, 7, 11). LRH-1 is highly expressed in tissues of endodermal origin and its expression is essential for normal liver, intestine, and pancreas function. LRH-1 has also been shown to be expressed in the ovary and adipose tissue.
In a recent report, Chand and colleagues investigated the mechanism of action of LRH-1 in invasive breast cancer cells. They found that LRH-1 promotes motility and cell invasiveness in both ER-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells and similar effects were observed in non-tumorigenic mammary epithelial cells. Interestingly, both remodeling of the actin cytoskeleton and E-cadherin processing were observed when LRH-1 was over-expressed. These findings implicate LRH-1 in promotion of migration and invasion in breast cancer independent of estrogen sensitivity. Together these findings provided strong evidence that LRH-1 plays a significant role in tumor formation both in vitro and in vivo. Therefore, the identification of potent and selective LRH-1 inverse agonists may provide new approaches for the treatment of cancer.
1. Fayard, E., J. Auwerx, and K. Schoonjans, LRH-1: an orphan nuclear receptor involved in development, metabolism and steroidogenesis. Trends Cell Biol, 2004. 14(5): p. 250-60.
2. Galarneau, L., J.F. Pare, D. Allard, D. Hamel, L. Levesque, J.D. Tugwood, S. Green, and L. Belanger, The alpha1-fetoprotein locus is activated by a nuclear receptor of the Drosophila FTZ-F1 family. Mol Cell Biol, 1996. 16(7): p. 3853-65.
3. Kudo, T. and S. Sutou, Molecular cloning of chicken FTZ-F1-related orphan receptors. Gene, 1997. 197(1-2): p. 261-8.
4. Boerboom, D., N. Pilon, R. Behdjani, D.W. Silversides, and J. Sirois, Expression and regulation of transcripts encoding two members of the NR5A nuclear receptor subfamily of orphan nuclear receptors, steroidogenic factor-1 and NR5A2, in equine ovarian cells during the ovulatory process. Endocrinology, 2000. 141(12): p. 4647-56.
5. Broadus, J., J.R. McCabe, B. Endrizzi, C.S. Thummel, and C.T. Woodard, The Drosophila beta FTZ-F1 orphan nuclear receptor provides competence for stage-specific responses to the steroid hormone ecdysone. Mol Cell, 1999. 3(2): p. 143-9.
6. Ellinger-Ziegelbauer, H., A.K. Hihi, V. Laudet, H. Keller, W. Wahli, and C. Dreyer, FTZ-F1-related orphan receptors in Xenopus laevis: transcriptional regulators differentially expressed during early embryogenesis. Mol Cell Biol, 1994. 14(4): p. 2786-97.
7. Lavorgna, G., H. Ueda, J. Clos, and C. Wu, FTZ-F1, a steroid hormone receptor-like protein implicated in the activation of fushi tarazu. Science, 1991. 252(5007): p. 848-51.
8. Li, Y., M. Choi, G. Cavey, J. Daugherty, K. Suino, A. Kovach, N.C. Bingham, S.A. Kliewer, and H.E. Xu, Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1. Mol Cell, 2005. 17(4): p. 491-502.
9. Solomon, I.H., J.M. Hager, R. Safi, D.P. McDonnell, M.R. Redinbo, and E.A. Ortlund, Crystal structure of the human LRH-1 DBD-DNA complex reveals Ftz-F1 domain positioning is required for receptor activity. J Mol Biol, 2005. 354(5): p. 1091-102.
10. Krylova, I.N., E.P. Sablin, J. Moore, R.X. Xu, G.M. Waitt, J.A. MacKay, D. Juzumiene, J.M. Bynum, K. Madauss, V. Montana, L. Lebedeva, M. Suzawa, J.D. Williams, S.P. Williams, R.K. Guy, J.W. Thornton, R.J. Fletterick, T.M. Willson, and H.A. Ingraham, Structural Analyses Reveal Phosphatidyl Inositols as Ligands for the NR5 Orphan Receptors SF-1 and LRH-1. Cell, 2005. 120(3): p. 343-355.
11. Luo, X., Y. Ikeda, and K.L. Parker, A cell-specific nuclear receptor isa essential for adrenal and gonadal development and sexual differentiation. Cell, 1994. 77(4): p. 481-90.
late stage, powders, purchased, SAR, medchem, MDRUN, dose response, dose, titration, crc, triplicate, HTS, high throughput, 1536, Nuclear receptor, NR, CYP7A promoter-binding factor; alpha-1-fetoprotein transcription factor; b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus; fetoprotein-alpha 1 (AFP) transcription factor; hepatocytic transcription factor; liver receptor homolog 1; liver receptor homolog-1; nuclear receptor NR5A2; nuclear receptor subfamily 5 group A member 2, LRH1, liver, activator, inverse agonist, transcriptional assay, luciferase, luminescence, Scripps Florida, The Scripps Research Institute Molecular Screening Center, SRIMSC, Molecular Libraries Probe Production Centers Network, MLPCN.
Assay Overview:
The purpose of this assay is to confirm activities of powder samples of compounds deemed promising after beeing titrated in the LRH-1, SF-1, VP16 and StAR promoter assays (AIDs 651970, 651968, 651973 and 651969, respectively).
This assay monitors LRH-1 activity, as measured by inhibition of promoter activity of the Steroidogenic acute regulatory protein (Star). In this assay, HEK293T cells, co-transfected with a full length LRH-1 construct in a pSport6 vector backbone (pS6-LRH-1) and a Star-luciferase reporter construct are incubated for 24 hours with test compound. StAR is a transport protein that regulates cholesterol transfer within the mitochondria, which is the rate-limiting step in the production of steroid hormones. It is primarily present in steroid-producing cells, including theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex. Compounds are tested in triplicate using a 10-point 1:3 dilution series starting at a maximum nomimal test concentration of 36 uM.
Protocol Summary:
Seven million HEK293 cells were seeded in T-175 flasks 23 mL of DMEM media supplemented with 10% v/v fetal bovine serum and 1% v/v Anti-Anti. Flasks were then incubated for 48 hours at 37 C, 5% CO2 and 95% relative humidity (RH). The day prior to run the assay, cells were harvested using TrypLE, resuspended in fresh media at a density of 1 million cells per mL and seeded into new T-175 flasks (23 mL per flask). After being allowed to attach for one hour at 37 C, 5% CO2 and 95% RH, cells were transfected with 1 mL of preincubated mix of serum-free OptiMEM containing 23 ug of the StAR-Luc reporter plasmid, 11.5 ug of the LRH-1 expression vector and 80 uL of transfection reagents. Twenty four hours post transfection, cells were harvested using 5 mL of TrypLE and resuspended at a concentration of 750,000 cells per mL in phenol-red free DMEM media supplemented as described above.
The assay was started by dispensing 5 uL of cell suspension into each well of a white, solid-bottom 1536-well plate using a flying reagent dispenser (i.e. 3,750 cells per well). The first two columns received cells transfected with the reporter plasmid only (no cotransfection with the LRH-1 expressing vector) as a control for background luminescence (no LRH-1 cells). Cells were then treated with 18 nL/well of test compounds, DMSO as a negative control (final concentration 0.36%) or SR-1848 as a positive control (36 uM final for full inhibition and 1.2 uM for 50% inhibition) using a PinTool transfer unit (GNF). Plates were then placed in the incubator at 37 C, 5% CO2 and 95%RH. Twenty four hours later, plates were removed from the incubator and equilibrated to room temperature for 10 minutes. Luciferase was detected by adding 5 uL per well of ONE-Glo luciferase detection reagent. After a 15 minutes incubation time, light emission was measured with the ViewLux reader (PerkinElmer). The percent inhibition of each test compound was calculated as follows:
The percent inhibition for each compound was calculated as follows:
%_Inhibition = ( 1 - ( Median_Positive_Control - Test_Compound ) / ( Median_Positive_Control - Median_Negative_Control ) * 100
Test_Compound is defined as wells containing test compound.
Low_Control is defined as wells containing DMSO.
High_Control is defined as wells containing cells transfected with the reporter only (no LRH-1 expressed).
For each test compound, percent inhibition was plotted against compound concentration. A four parameter equation describing a sigmoidal dose-response curve was then fitted with adjustable baseline using Assay Explorer software (Accelrys Inc). The reported IC50 values were generated from fitted curves by solving for the X-intercept value at the 50% activation level of the Y-intercept value. In cases where the highest concentration tested (i.e. 36 uM) did not result in greater than 50% activation, the IC50 was determined manually as greater than 36 uM.
PubChem Activity Outcome and Score:
Compounds with an IC50 greater than 10 uM were considered inactive. Compounds with an IC50 equal to or less than 10 uM were considered active.
Any compound with a percent activity value < 50% at all test concentrations was assigned an activity score of zero. Any compound with a percent activity value >= 50% at any test concentration was assigned an activity score greater than zero.
Activity score was then ranked by the potency of the compounds with fitted curves, with the most potent compounds assigned the highest activity scores.
The PubChem Activity Score range for active compounds is 100-65, and for inactive compounds 63-0.
List of Reagents:
HEK-293 cells (ATCC, part CRL-1573)
DMEM media (Invitrogen, part 11965)
Fetal Bovine Serum (Hyclone, part SH30088.03)
Anti-Anti (Gibco, part 15240)
TrypLE (Invitrogen, part 12604)
T-175 flasks (Falcon, part 353112)
StAR-Luc plasmid (Assay Provider)
LRH-1 expression vector (Assay Provider)
TransIT 293 transfection reagent (Mirus Corporation, part MIR-2700)
ONE-Glo luciferase reagent (Promega, part E6130)
White, solid-bottom 1536-well plates (Greiner, part 789173)
Due to the increasing size of the MLPCN compound library, this assay may have been run as two or more separate campaigns, each campaign testing a unique set of compounds. All data reported were normalized on a per-plate basis. Possible artifacts of this assay can include, but are not limited to: dust or lint located in or on wells of the microtiter plate, and compounds that modulate well luminescence. All test compound concentrations reported above and below are nominal; the specific test concentration(s) for a particular compound may vary based upon the actual sample provided by the MLSMR.
Categorized Comment - additional comments and annotations
From BioAssay Depositor:
Assay: CurveFit [1]: Equation: =( ( [Maximal Response] * [Concentration]^[Hill Slope] ) / ( [Inflection Point Concentration]^[Hill Slope] + [Concentration]^[Hill Slope] ) ) + [Baseline Response]
Assay: CurveFit [1]: Mask: Excluded Points
Assay: Dictionary: Version: 0.1
From PubChem:
Assay Format: Cell-based
Assay Cell Type: MDA-MB-231
Result Definitions
* Activity Concentration. ** Test Concentration.
Additional Information
Grant Number: CA134873
Data Table (Concise)