text:
Book your room at Best Western Lamplighter Inn & Suites at SDSU
Standard Room, 2 Queen Beds, Non SmokingMax Occupancy: 4 guests (up to 3 children)
Standard Room, 2 Queen Beds, Non Smoking, Refrigerator & MicrowaveMax Occupancy: 4 guests (up to 3 children)
Standard Room, 1 King Bed, Accessible, Non SmokingMax Occupancy: 2 guests (up to 1 child)
Standard Room, 1 Queen Bed, Non SmokingMax Occupancy: 4 guests (up to 3 children)
Standard Suite, 1 King Bed, Non Smoking, Refrigerator & MicrowaveMax Occupancy: 4 guests (up to 3 children)
Standard Suite, 2 Queen Beds, Non SmokingMax Occupancy: 6 guests (up to 5 children)
Get an overview of this hotelFamily-friendly hotel with free breakfast and outdoor pool
This family-friendly San Diego hotel is within 2 mi (3 km) of San Diego State University Art Gallery and San Diego State University. Mission Valley Mall and Mission Trails Regional Park are also within 6 mi (10 km).
Hotel Features
Along with an outdoor pool, this hotel has laundry facilities and a 24-hour front desk. Free continental breakfast, free WiFi in public areas, and free self parking are also provided. Other amenities include an arcade/game room, free newspapers, and barbecue grills.
Room Amenities
All 60 rooms offer free WiFi, coffee makers, and TVs with cable channels. Other amenities available to guests include DVD players, hair dryers, and phones.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
Guests can enjoy a complimentary breakfast. Public areas are equipped with complimentary high-speed wireless Internet access. This family-friendly hotel also offers an outdoor pool, barbecue grills, and an arcade/game room. Onsite self parking is complimentary.
- 24-hour front desk
- Arcade/game room
- Barbecue grill(s)
- Pool umbrellas
- Free newspapers in lobby
- Free breakfast
- Free self parking
- Free WiFi
- Laundry facilities
- Outdoor pool
- Pool sun loungers
- Safe-deposit box at front desk
- Total number of rooms - 60
Family Friendly Amenities
- Arcade/game room
- DVD player
- Free Wi-Fi
- Laundry facilities
- Outdoor pool
- Rollaway/extra beds available
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking
Room Amenities
- Air conditioning
- Cable TV service
- Coffee/tea maker
- Free toiletries
- Cribs/infant beds available
- Daily housekeeping
- Desk
- DVD player
- Free WiFi
- Hair dryer
- Iron/ironing board
- Phone
- Premium TV channels
- Private bathroom
- Rollaway/extra beds available
- Shower/tub combination
- Television
Where to Eat
Guests are offered a complimentary continental breakfast.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Accessible path of travel
- Accessible parking
- In-room accessibility
Hotel Policies
Check-in time starts at 3 PM
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! Children under 12 years old stay free when using existing bedding.
- Rollaway/extra beds are available.
- Cribs (infant beds) are available.
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
We should mention
Children 12 years old and younger stay free when occupying the parent or guardian's room, using existing bedding.


text:
- About Us
- Our Work
- Our Certification
- Our Publications
- Our Blog
- Take Action
|Get Listed in the
National Green Pages™!
|Your business membership in Green America includes a listing in the National Green Pages™.
|Apply today »
Green America certifies businesses that are committed to using business as a platform for social change. Since 1982, Green America has evaluated over 8,000 small businesses.
We've helped leading green companies like Seventh Generation, Honest Tea, and Clif Bar take off in the marketplace. We connect people to entrepreneurs building sustainable enterprises from the ground up and Main Street businesses that serve their communities.
Businesses: Earn your Green Business Certification »
Green America certifies businesses that are:
We certify green businesses with rigorous Green Business Standards in over forty industries.
Look for the Certified Business Seal to find green products and services from thousands of businesses committed to social and environmental responsibility.
Businesses earn this seal after staff at Green America's Green Business Network reviews and approves their application.
Gold Certified
Businesses earn this seal after completing additional work beyond general certification. Their application has been reviewed and approved by the Standards and Certification Committee, appointed by Green America's Board of Directors.
Our Green Business Certification not only helps you find green products and services, but it also helps green businesses implement ongoing sustainablility work smartly and strategically using our online platform. We provide the tools to help businesses turn their social and environmental mission into action.
Businesses: Discover how to get certified »
Businesses that have attained the Gold Certified Business Seal have successfully completed an evaluation by the Green America Standards Committee.
The Green America Standards Committee conducts reviews using data provided by the business and independent sources to certify that a business has moved beyond general sustainability actions into a deeper focus on their core business area.
Operating since 1982, the Standards Committee is appointed by and reports to Green America's Board of Directors.
Discover ...

subset: commoncrawl


text:
Up to 40% Off Blackpool Deals at Amazon LocalGet This Offer
- i View Blackpool Pleasure Beach
Want to visit Britain's favourite seaside town? Check out the latest local offers now to see if you can save money on your visit.
By browsing the MyVoucherCodes site you're agreeing to the use of cookies. Cookies are tiny bits of data sites store in your web browser to make your online experience better.Not sure about cookies?
Whether you're looking for thrilling rides and attractions, a ripping good time bowling, to be scared out of your wits in the Horror Crypt, to have your fortunes read, Blackpool Pleasure Beach in Lancashire has it all. So if you're thinking about visiting the popular seaside town of Blackpool,l check out Blackpool Pleasure Beach discount codes, to get in to the park for less! Bring the family or a group of friends and you'll get a great deal on your tickets, too! We've got incredible offers for a wide range of other local attractions such as Blackpool Tower, Nickelodeon Land, Blackpool Zoo and many more, as well as plenty of accommodation and travel options.
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What is the park's open season? What are their hours?
The park is open February through December and is opened every day in the summer. Please consult their website or customer service teams for exact times.


text: These socks are nice. They fit my large feet, feel good in my boots, and keep my feet cool compared to traditional white and black socks. The only reason I did not give it five stars are because of the air holes that run up the calfs of the socks. Even though they provide great airflow to the legs and feet, I believe it opens up the possibility for premature holes in the socks after a few rides and washes. But overall, great socks! I totally recommend these to anyone!


text:
Property for sale near Plaistow Street, Lingfield RH7
Property type
More options
- Pre-owned / New homes No preference
- Ownership type No preference
- Retirement No preference
- Buyer incentives No preference
Guide price £650,000 Just added
3 bed flat for saleHigh Street, Lingfield RH7
Development Opportunity - A fantastic detached building currently comprising three one bedroom flats and a spacious ground floor with the potential to convert into a further two properties. The property benefits from three parking spaces, a garden and ... More details
Note: Distances are straight line measurements
- Lingfield (0.4 miles)
- Dormans (1.4 miles)
Marketed by Move Revolution, RH1. Call 01737 339305Offers in region of £399,950
3 bed terraced house for saleEliot Place, Crowhurst Place, Lingfield, Surrey RH7
Three bedrooms – Open plan living/dining room – Fitted kitchen with aeg appliances – Master bedroom with en suite shower room – Private garden – Garage and parking – Communal lake, woodland and... More details
Note: Distances are straight line measurements
- Lingfield (0.5 miles)
- Dormans (1.4 miles)
Marketed by Mansell McTaggart - East Grinstead, RH19. Call 01342 602896
Latest Plaistow Street, Lingfield RH7 property for sale
Zoopla is one of the UK's leading property portals, helping you to find property for sale and to rent and make smarter decisions when buying and renting homes in the UK. Discover information on homes in Plaistow Street, Lingfield RH7 by researching Plaistow Street, Lingfield RH7 property values, Plaistow Street, Lingfield RH7 house prices paid, our Plaistow Street, Lingfield RH7 property market overview and find Plaistow Street, Lingfield RH7 agents.


text:
Charlie Sheen's ex-wife pleads guilty to drug charge
- Photo: Ryan Fu/RSA/ZOJ/WENN1 of 13
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ASPEN, Colo. (AP) -- Charlie Sheen's ex-wife has pleaded guilty to a reduced cocaine drug charge under a deferred judgment that means her record will remain clean after she serves a year of probation.
RELATED: Charlie Sheen and other terrible drivers
Court documents show Brooke Mueller appeared Monday in district court in Aspen to plead guilty to felony drug possession of four grams or less. In exchange, prosecutors agreed to drop the more charge of serious possession with intent to distribute, as well as a misdemeanor third-degree assault charge.
Sentencing is scheduled for May 7.
RELATED: Celebrities Who Need a Fresh Start in 2012
Charges stemmed from Mueller's arrest Dec. 3 following a woman reporting being assaulted at a nightclub.
Sheen and Mueller divorced last year after Sheen was arrested on suspicion of assaulting her in 2009. He pleaded guilty to misdemeanor assault and completed his probation in 2010.
RELATED: Denise Richards' Highs and Lows
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text:
Gary Varvel by Gary Varvel
- August 27, 2013
- From Beginning
- Previous feature
- Next feature
- Current
Comments (22) (Please sign in to comment)
Another Liberal 'toon that's misleading. Republicans don't want to shut down the government, ONLY Obamacare! It's the LEFT that's threating all or nothing.
A.) Nothing is going to happen if the government shuts down. B.) Only the "Establishment Republicans" (McCain, Boener, etc) would have this point of view. I say go ahead and shut it down; somebody needs to hit the reset button…
The government has shut down 18 times in the past. How come we still exist?
Think about it, the government shuts down every friday, every holiday, and leaves the bare minimum of people around to take care of the emergencies.
Congress shuts down for "recess", are we the worse for wear?
During a government "shutdown", only "non essential" personnel are let go. LOL NON ESSENTIAL?? Why do we have them if we really do not need them?
As usual, the American people are being taken for a ride…….. Chicago style.
If you think Varvel is a liberal, I suppose you think Attila is middle of the road?
Amen brother.
Don't tell me you're surprised. The American people aren't for "the American people" anymore. Good luck finding any unity- it's all "we're the only REAL Americans" and "let them rot; we're all that matters".
Divided we fall….
Truest words.
Ani't that the truth.
Elephants are supposed to have good memories but not Dumb(o).
Whoa. Varvel thinks the RepubliCons can do something wrong? 'Cuse me, I'm gonna go lie down.
No misreading about it. Remember 1994 and then Speaker of the House Newt Gingrich? The same thing was pulled then. The Republicans lost terribly in the next elections. It could happen again. The Republicans are barely holding on to what little they could scam in the House.
No reset button to hit. This isn't a computer program. And the govt doesn't completely shut down, but the weak will suffer.
Rep Cons = favoritism of the feudal lords—-the ultra rich, damn everyone else.
Vavel does, I will admit, occasionally gets it right, as he does in this one, even though he IS a far-right cartoonist most of the time, he's not a complete idiot, like some on GC (hmm LIsa comes to mind) or the far right extremists of the "no nothing" wing of the current republican (deliberate lower case) Party who post here.
Gary, good 'toon.
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text:
McDonald's: Veggieburger
New: The Veggieburger
Advertising Agency: Neue Digitale/Razorfish, Berlin, Germany
Creative Director: René Lamberti
Copywriter: Alexander Ardelean
Art Director: Mauricio Franicevich Garcia
Photographer: Jo Kirchherr c/o Banrap Photographers
34 comments
Wow, now I really want meat. This ad was well intentioned, but it just feels bizarre.
Jeff Greenhouse | http://www.201proof.com | http://www.JeffGreenhouse.com
If you're a vegetarian, add two stars; if not, subtract two.
As a vegetarian, I don't want to be reminded of the fact your core product is meat.
You all talk about veggies as if they had fobia to the animals. Pfff! This is a juck!! Even Veggies understand the juck
This is weird... it´s the same idea of the Greenpeace campaign about genetically modified food. Just for the reflexion.
Luis Maram
Surely the vegimal should be eating a piece of meat, rather than lettuce.
This is not the same final Art from the Campaign. Who changed the proportions and added the ugly grey frame???
The Headline is also gone... :(
This is an interesting example of perspective by incongruity. If McDonald's was trying to make veggie burgers appetizing to their target audience (vegetarians), perhaps they should reconsider the idea of arranging the vegetables in the shape of a cow..
I think McDonalds didnt want to reach the target group of vegetarians.
The idea was to say: "There arent only unhealthy meat products at McDonalds.
Try the veggie product... it tastes as good as meat!"


text:
No. 14 A&M Looks To Move On From Upset To Auburn
COLLEGE STATION (AP) — Texas A&M is searching for a way to move on after this weekend's upset loss to Auburn.
The 45-41 defeat dropped the Aggies from seventh to 14th in the poll.
Now Texas A&M has to see if Johnny Manziel's right shoulder is OK and figure out how to improve its porous defense before hosting Vanderbilt on Saturday.
Manziel missed a series in the fourth quarter against the Tigers with the shoulder injury before returning in the loss.
Coach Kevin Sumlin didn't provide an update on the severity of his injury after the game Saturday night, but didn't think it was a factor in the defeat.
(© Copyright 2013 The Associated Press. All Rights Reserved. This material may not be published, broadcast, rewritten or redistributed.)
Latest News:
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Top Trending:
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- PHOTOS: Your Pet Pictures


text:
Back to Previous Page
The Boss 302 returns to the road after 42 years.
Shaking Hands With The Devil
In this video the Flex picks off a modded Mustang GT.
Make Your "Five-Oh" Go Faster and Smoother
If you love Ford performance, this is the place for you!
King of the Road
A Race Car For The Streets
Born on California's highways, the Mustang SSP offered police a high-performance alternative.
How to get up-close and personal to find the finest Ford Mustang you can afford.
A visual guide to telling one vintage Pony Car from its model-year stable mates.
A visual feast for Mustang lovers.
Mustang's third body style emerges.
A Real Jekyll & Hyde Ride
Daytona Speedway was a fitting corral for these ponies.
Upgrading a 1966 Mustang's Baer system from two-piston PBR caliper to six-piston Baer caliper.
Tulsa Raceway Park thundered with Ford power!
Carving corners with Ford power!
Walking around the paddock at Hallett Motor Racing Circuit
The Ultimate Ford Family Reunion
Fords invade downtown Tulsa's Brady Arts District
The power plants that gave the Mustang its gallop.
A great mix of old and new meet at this year's Atlanta auto show.
Walking around Tulsa Raceway Park
The capstone of a great event.
Families, fun and Mustangs at Myrtle Beach.
We see how the "other" ones to watch did this year.
Celebrating a glorious racing past, these cars are finally getting the respect they deserve.
Some Show Cars Defy Description
Lonny Childress of Gateway Classic Mustang pilots their 5.0L Coyote mod motor powered '69 Mustang Sportsroof around Hallett Motor Racing...
There was no raining on this parade.
Once you are hooked it is hard to go back.
AutoTrader Classics Editor Brian Medford travels to Streetside Classics in Powder Springs, GA to talk about a little bit of British history, the Mini.
The heart of the beast. Check out the cool custom touches that make these powerplants unique!
A fitting finale for an amazing Ford filled event!
It takes something really unique to stand out at this show and these rides do just that!
The High Rollers
Some cars defy categories, so here is a bit of everything!
Fantastic fiberglass and a few rarities that left even the experts puzzled.
Some of the finest examples of the automotive craft.
The 2010 Detriot AutoRama Ridler Award Great 8!
These kids show what the next generation can do!
Here is a brief chance to see these machines sitting still.
These show stoppers are the heart and soul of the event!
The best old haulers of SEMA
The Florida heat had nothing on these paint jobs.
From horseless carriages to elegant town cars, these steeds showcase the coachbuilder's craft.
New car plus old styling equals retro cool!
It was a winning day for AutoTrader Classics and the Atlanta Braves!
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text:
11 Ways To Cook Bacon That Will Probably Kill You
Are you ready to experience the essence of pure flavor?
1. Pan Fried Bacon-Wrapped Steak
2. Epic Bacon And Steak Bread Bowl Panini
3. I don't even know WHAT you'd call this thing
4. Barbecue Bacon Burger Log
5. Bacon Turtle
6. Eggs Of Awesome
7. The Bacon Log
8. The Bacon And Cheese Stuffed Pizza Burger
9. The Cheesy Bacon Log
10. The Cheesy Bacon Sausage
11. Epic Bacon Grilled Cheese
Preview Your Response
In The News Today
- California Republican Kevin McCarthy has dropped out of the race to be the next speaker of the U.S. House. ›
- The United Nations has proposed a national unity government for Libya after peace talks between the north African country's two rival factions. ›
- It could take more than a year for Volkswagen to fix all its cars with diesel engines rigged to evade emissions tests, the company's U.S. CEO said. ›


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text:
Here are the beta cameos: Tcrf.net
Hiya! Thanks for visiting my fine mess of a profile. Feel free to look around. I currently play on Steam and a bit of C&C, everything else on the side (playtime wise). I have a lot of other hobbies, but I'm planning to start on learning how to do 3D Modelling, more about mod making and using SFM. Known as MsD IV around places. A bit more than lazy so to say. Finally back after a long while, I show up every now and then
Here are the beta cameos: Tcrf.net
Oh awesome XD
Good to see you back!
How about this? Nmods.wikkii.com
I wonder what that would took like.
Obviously :P
Here's a PC Version of Pro:Gen 2.6 if you're still finding it.
Hey it's been a long time already I'm wondering if you can read all the comments since things have very been different now...
You can since both mods have launchers.
Did anyone see the SCUD storm timer?
I'm sure they're still gonna keep him. :P
Looks good!
I see a combination of different models.
"Don't get cocky,Iron Dragons don't mean it's over yet"
or better?
LOL they're intelligent alright!
This isn't public,this was in the game files.
It already looks better than the Iron Dragon of other mods!
Thanks!I have it now.
Is that a mission called "Cradle Summit" in CNCDen?
I like the balances for this tank and the fact that it doesn't match the Scorpion...
Do I see a flagpole?
It looks like a Russian jet from ROTR amirite?
Actually there is
Ahh good finally it has come!
I can tell that you watched the FARMVILLE commercial by Tobuscus.
have you guys even validated your accounts?
@yash Perhaps your scripts are corrupted.Check them first.


text:
Franklin County Jail, WSP Trooper, Cleared in Wrongful Death Suit
Benton City Man's Cause of Death Ruled "Undetermined" By Coroner
Pendleton Father Jailed In Connection with 'Throwing' Death of Child
Death Of Two Rivers Prison Inmate in Umatilla Brings Investigation
Grandview Teen Dies As Result of Illegal Gun Sale
Murder Charges Delayed in Death of Kennewick Toddler Dec. 23
Oregon Prison Inmate Dies Thursday, Investigation Underway
Driver Dies After 500 Foot Plunge Over Embankment – Investigation Continues
Oregon Farmer Eaten by His Own Hogs
In Riverton, Oregon, a 70-year-old farmer's family found his dentures and remnants of his body in the hog pen. For unknown reasons he fell into the pen and was eaten by the 700-lbs. animals.


text:
Page 1
Lymphoid Priming in Human Bone Marrow Begins Prior to CD10
Expression with Up-Regulation of L-selectin
Lisa A. Kohn1, Qian-Lin Hao2, Rajkumar Sasidharan3, Chintan Parekh4, Shundi Ge1, Yuhua
Zhu1, Hanna K.A. Mikkola3,5, and Gay M. Crooks1,4,5
1Department of Pathology & Laboratory Medicine, University of California, Los Angeles
2Division of Research Immunology & Bone Marrow Transplantation, Children's Hospital Los
3epartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles
4Department of Pediatrics, University of California, Los Angeles
5Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of
California, Los Angeles
The expression of CD10 has long been used to define human lymphoid commitment. We report a
unique lymphoid-primed population in human bone marrow that was generated from
hematopoietic stem cells (HSCs) before the onset of CD10 expression and B cell commitment.
This subset was identified by high expression of the homing molecule L-selectin (CD62L).
CD10−CD62Lhi progenitors possessed full lymphoid and monocytic potential, but lacked
erythroid potential. Gene expression profiling placed the CD10−CD62Lhi population at an
intermediate stage of differentiation between HSCs and lineage-negative (Lin−) CD34+CD10+
progenitors. L-selectin was expressed on immature thymocytes and its ligands were expressed at
the cortico-medullary junction, suggesting a possible role in thymic homing. These studies
identify the earliest stage of lymphoid priming in human bone marrow.
Although much is known about the identity of progenitor stages in murine lymphopoiesis,
considerably less is understood about the critical stages of lymphoid commitment of human
hematopoietic cells. Early models developed from murine studies assumed strictly
dichotomous pathways of lineage commitment1. These concepts have evolved more recently
into models of gradual loss of lineage potential that can occur via multiple alternative
pathways, although the physiological relevance of lineage potential revealed in certain in
vitro assays continues to be debated2–5. A stage in which murine bone marrow (BM)
progenitors are "lymphoid primed" prior to complete loss of myeloid potential has been
Correspondence should be addressed to G.M.C. (email@example.com) Gay M. Crooks, M.B.B.S. Department of Pathology &
Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, MS# 951732, 10833 Le Conte
Avenue, Los Angeles CA 90095-1732, Phone: (310) 206-0205, Fax: (310) 206-0356, email@example.com.
The microarray data were deposited in NCBI's Gene Expression Omnibus database (Accession# GSE35685) and are accessible
through the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=ztenfowesgyagjk&acc=GSE35685
Author contributions
LAK designed, performed and analyzed experiments and wrote the paper, Q-LH designed, performed and analyzed experiments, RS
performed bioinformatics on microarray data, SG and YZ assisted in experiments, CP performed experiments, HKAM supervised
bioinformatics analysis, GMC designed and analyzed experiments and wrote the paper.
The authors have no competing financial interests to declare.
NIH Public Access
Author Manuscript
Nat Immunol. Author manuscript; available in PMC 2013 April 01.
Published in final edited form as:
Nat Immunol. 2012 October ; 13(10): 963–971. doi:10.1038/ni.2405.
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defined based on expression of the FLT3 cell surface receptor, and termed the Lymphoid-
primed Multipotent Progenitor (LMPP)2.
Critical species-specific differences create challenges when translating knowledge of
cellular hierarchies derived from murine studies to the specifics of human hematopoiesis6. In
addition, the source and stage in ontogeny of human hematopoiesis can influence the
functional capacity, surface immunophenotype and transcriptional profiles of the cells under
study6–8. Most studies of the earliest progenitor stages in human hematopoiesis have used
neonatal umbilical cord blood as the source of hematopoietic cells. However, to understand
how lymphopoiesis is regulated during steady-state adult hematopoiesis it is necessary to
directly study hematopoietic stem cells and progenitors from postnatal human BM8,9.
The stepwise process of lymphoid differentiation from multipotent hematopoietic stem cells
(HSCs) in human BM has been assumed to begin with the expression of the cell surface
antigen CD10 (aka CALLA, MME) on CD34+ cells10. However, while CD34+lin−CD10+
cells can give rise to cells of all lymphoid lineages, subsequent work has shown that CD10
expression on progenitors is associated with a strong bias toward B cell potential and
minimal T and natural killer (NK) cell potential11,12. CD34+lin−CD10+ cells that lack
expression of CD24 are precursors of the CD34+lin− CD10+CD24+ population, but
nonetheless show molecular evidence of B cell commitment with expression of PAX5,
EBF1 and VPREB12. Therefore, to understand the progenitor hierarchy of human lymphoid
commitment, we sought to identify a stage of lymphoid priming that precedes B lymphoid
commitment, either prior to or independent of CD10 expression.
L-selectin (CD62L) is expressed on lymphocytes and mediates homing to peripheral
lymphoid organs13. Recent studies have reported that up-regulation of CD62L expression on
c-Kit+lin−Sca1+ murine BM cells correlates with loss of erythroid and megakaryocyte
potential and efficient thymic engraftment14–16. In this study we have identified a
CD34+lin−CD10− progenitor subpopulation in human BM that expressed high amounts of
L-selectin and was devoid of clonogenic myeloid or erythroid potential. In stromal cultures
these cells were able to generate B, NK and T cells as well as monocytic and dendritic cells,
similar to the previously described LMPPs in murine BM2. CD34+lin−CD10−CD62Lhi
("CD10−CD62Lhi") cells rapidly engrafted immune-deficient mice, producing B and
myeloid cells. Despite evidence of lymphoid skewing, comprehensive molecular analysis
revealed that CD10−CD62Lhi cells not only lacked B cell specific transcripts, but also had
not initiated DNA recombination based on absent RAG1, RAG2 and minimal DNTT
expression. Genome-wide expression and functional analysis placed the CD10−CD62Lhi
progenitor population as a developmental intermediate between the multi-potent
CD34+lin−CD38− population and the CD34+lin−CD10+ lymphoid progenitor.
We also find that primitive lymphoid-restricted CD34+CD1a− progenitors in human thymus
expressed CD62L, and that the vasculature at the cortico-medullary junction of human
thymus expressed ligands for CD62L, suggesting the possibility that L-selectin may play a
role human thymic homing. We propose that the CD10−CD62Lhi progenitor in BM
represents the earliest stage at which adult human progenitors become lymphoid-primed.
The identification of this progenitor population will facilitate a more complete
understanding of the regulation of lymphoid commitment from HSCs during normal and
aberrant human hematopoiesis.
Kohn et al.
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CD7 expression does not define lymphoid commitment
In view of previous studies by our group and others linking CD7 expression to early stages
of lymphoid commitment in umbilical cord blood17–20, we first investigated if expression of
CD7 was sufficient to identify human lymphoid commitment in bone marrow, independent
of CD10 expression. Examination of lineage-depleted cells revealed that the
CD34+lin−CD38−CD7+ population previously identified in umbilical cord blood17 was not
detectable in human BM (Supplementary Fig. 1a). However, as previously noted7, low
expression of CD7 was detected on a small (2.8 ± 0.6%, n = 5) population of
CD34+lin−CD38+ human BM cells, most of which did not co-express CD10 (Fig. 1a).
Clonogenic assays demonstrated that CD7 expression alone was insufficient to define
lymphoid restriction within the CD34+lin−CD10− population of BM; non-lymphoid
clonogenic cells, particularly erythroid progenitors, were readily detectable in the
CD34+lin−CD10−CD7+ population by Colony Forming Unit-Cell (CFU-C) assay (Fig. 1b).
Consistent with previous studies in BM and umbilical cord blood7,10–12,21,
CD34+lin−CD10+ progenitors were devoid of clonogenic myeloid and erythroid progenitors
(Fig. 1b).
L-selectinhi progenitors do not possess CFU potential
CD45RA has previously been shown to be expressed on various lymphoid
progenitors10,17–19 and granulocyte-macrophage progenitors (GMPs)22. Analysis of the
CD34+lin−CD10− subpopulation demonstrated the presence of both CD45RA− and
CD45RA+ fractions; in contrast all CD34+lin−CD10+ cells expressed CD45RA (Fig. 1c).
Erythroid potential was depleted, but clonogenic myeloid progenitors (CFU-GM) were still
readily detectable, in the CD34+lin−CD10−CD45RA+ population (Fig. 1d). As expected,
erythroid potential was high in CFU-C from megakarocytic-erythroid progenitors (MEPs)
and common myeloid progenitors (CMPs) (Fig. 1d), neither of which express CD45RA.
Further refinement of the CD10−CD45RA+ population was necessary to identify those cells
that lacked clonogenic myeloid potential. L-selectin (CD62L) is a cell surface receptor that
mediates lymphocyte homing to peripheral nodes13 and which is expressed on certain
murine BM progenitors that lack erythroid or megakaryocytic potential14. Analysis of the
CD34+lin−CD10− CD45RA+population demonstrated that although most cells dimly
expressed CD62L, a distinct subpopulation (9 ± 1.5%, n = 14) of
CD34+lin−CD10−CD45RA+ cells in normal human BM highly expressed CD62L (Fig. 1c).
Functional screening of CD34+lin− fractions in CFU-C assay demonstrated that only the
CD34+lin−CD10−CD45RA+CD62Lhi ("CD10−CD62Lhi ") population and the
CD34+lin−CD10+("CD10+") population were devoid of clonogenic myelo-erythroid
potential (Fig. 1d, Supplementary Table 1). Of note, the CD34+lin−CD10−CD45RA+
population that expressed intermediate amounts of CD62L contained low but detectable
CFU-C potential, suggesting that progressive loss of multi-potency correlates with
increasing CD62L expression (population B, Supplementary Fig. 1b,c).
CD10+ cells expressed low or undetectable amounts of CD62L, and the CD34+lin−CD38−
population, which is highly enriched for HSC and multipotent progenitor cells (MPPs),
showed intermediate expression of CD62L (Fig. 1e, Supplementary Fig. 1b). Notably,
CD10− CD62Lhi cells did not express CD7 (Fig. 1f). Thus, the progenitor subset with
highest CD62L expression expressed neither CD10 nor CD7, markers previously relied
upon for the isolation of human lymphoid progenitors. Analysis of BM from 20 different
individuals from infancy to adulthood consistently showed the presence of CD10− CD62Lhi
cells (Supplementary Fig. 2a–c).
Kohn et al.
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Lymphoid and monocyte potential of CD10− −CD62Lhi cells
Culture in lymphoid conditions demonstrated that the CD10− CD62Lhi population robustly
generated both B and NK cells (Fig. 2a). Consistent with previous studies, CD10+ cells (all
of which were CD19− through lineage depletion) generated mostly B cells with relatively
weak NK potential11. Cell output under B-NK lymphoid conditions tended to be higher in
cultures initiated with CD10− CD62Lhi than with CD10+ cells (Fig. 2b). Following in vitro
culture under T cell conditions, CD10−CD62Lhi cells generated cells with the
immunophenotype typical of thymocytes (expressing CD1A, CD7, CD4, CD8, CD3,
TCRαβ)23 (Fig. 2c,d and Supplementary Fig. 3), and expressing the T cell associated genes
TCF7, GATA3, DNTT and RAG1 (Supplementary Fig. 3) as well as CD56+ NK cells (some
of which co-expressed CD8). Cell output was significantly higher in T cell cultures initiated
with CD10−CD62Lhi than with CD10+ cells (P = 0.038) (Fig. 2e).
Although clonogenic myeloid cells were not detected in CFU-C assay, both the CD10+ and
CD10− CD62Lhi subsets were able to generate relatively low numbers of myeloid cells
when cultured on stromal layers; however cell output from both progenitor types was
significantly reduced relative to HSC-MPPs (P < 0.0001) (Fig. 2f). The majority of the non-
lymphoid cells generated in stromal co-culture from the CD10+ and CD10−CD62Lhi
populations were CD14+CD33+ monocyte-macrophages or CD209+CD1a+ dendritic cells
(Supplementary Fig. 4); CD66b+ granulocytes were uncommon. Erythroid differentiation
was rarely seen from CD10+ or CD10−CD62Lhi cultures but was robust in cultures from
CD38− HSC-MPPs.
Cloning efficiency of CD10−CD62Lhi cells in lymphoid cultures initiated with single cells
(~11%) and by limiting dilution analysis (1 in 5.3 for B-NK and 1 in 5.6 for T cell cultures)
(Fig. 3a,b) was similar to that of CD10+ cells (~12% from single cells). However lineage
analysis of clones demonstrated that the CD10−CD62Lhi population contained bi-potent B-
NK progenitors whereas the CD10+ population contained predominantly unipotent B cell
progenitors (Fig. 3c). Myeloid cells were detected in 86% of clones that could be assigned
lineages in B-NK conditions (Fig. 3d) and 97% of all clones assayed from T cell cultures
(Fig. 3e).
Consistent with the in vitro assays of lineage potential, intratibial transplantation of
CD10−CD62Lhi progenitors into immune-deficient (NOD–SCID–IL-2Rγ−/−) mice produced
rapid marrow engraftment of both myeloid and B lymphoid cells (Fig. 3f,g and
Supplementary Fig. 5). T lymphoid differentiation from non-self renewing progenitors
would not be expected in this xenogeneic adult mouse model.
In summary, functional assays showed that the CD10−CD62Lhi population possessed full T,
B and NK lymphoid potential, was less skewed toward the B lineage than the CD10+
population, and had greater T potential than CD10+ population. Although depleted of
clonogenic myelo-erythroid potential, some myeloid (mostly monocyte-macrophage and
dendritic cell) differentiation could be induced in stromal co-cultures from the
CD10−CD62Lhi population, and in short term engraftment assays. However, myeloid
potential was markedly decreased relative to that of HSC-MPPs and erythroid potential was
Differentiation stages of HSCs and lymphoid progenitors
In view of the lineage potential shown in the functional studies, we next explored the
relative stages of differentiation of the CD10−CD62Lhi and CD10+ populations when
compared to the most primitive CD34+lin−CD38− ("CD38-") HSC-MPP population.
Expression of the differentiation marker CD38 rose progressively from the CD34+CD38− to
the CD10−CD62Lhi population and was maximal in the CD10+ population (n = 14) (Fig.
Kohn et al.
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4a). Expression of the stem cell-associated receptors KIT, FLT3, ITGA6 (aka CD49f) and
PROM1 (aka CD133) was similar in CD38− and CD10−CD62Lhi populations but down-
regulated in CD10+ cells; THY1 (aka CD90) was most highly expressed on CD38− cells.
HLA-DR was up-regulated in both CD10−CD62Lhi and CD10+ progenitors (Fig. 4a). After
one week in lymphoid cultures, CD10+ cells differentiated and lost CD34 expression faster
than CD10−CD62Lhi cells (Fig. 4b). In addition, CD10−CD62Lhi cells were able to generate
CD34+CD10+ cells in vitro, suggesting that CD10−CD62Lhi cells are precursors to the
CD10+ population (Fig. 4b).
Principal components analysis performed on global gene expression data from microarrays
on three different BM samples also placed the CD10−CD62Lhi progenitors in an
intermediate position between the CD38− HSC-MPPs and the CD10+ progenitors (Fig. 4c).
Gene expression of CD10−CD62Lhi progenitors clustered hierarchically with CD38− HSC-
MPPs rather than with CD10+ progenitors (Supplemental Fig. 6a). In pairwise comparison
to HSC-MPPs, similar numbers of genes were up-regulated in CD10−CD62Lhi and CD10+
populations; approximately half of these up-regulated genes were common to both
progenitor types (Supplemental Fig. 6b). More than twice as many genes were down-
regulated in the CD10+ population than were down-regulated in the CD10−CD62Lhi
population, and most downregulated genes in CD10−CD62Lhi cells were also
downregulated in CD10+ cells (Supplemental Fig. 6b). Thus differentiation of HSC-MPPs
involves many shared molecular pathways but additional transcriptional modulation appears
to occur after the CD10−CD62Lhi stage during the generation of CD10+ cells.
Downregulation of HSC-associated genes in CD10− −CD62Lhi cells
Analysis of expression patterns of genes known to regulate critical hematopoietic stages of
differentiation was then performed by microarray and quantitative PCR (qPCR) to dissect
the molecular relationships amongst CD38−, CD10−CD62Lhi, and CD10+ populations. All
genes included in the heatmaps were at least 2-fold differentially expressed (P <0.05), and
belonged to one of 6 different expression patterns (clusters 1–6) (Fig. 5a,b). Known HSC-
related transcription factors (TAL1, GATA2, and PRDM16) were significantly down-
regulated in both the CD10−CD62Lhi and CD10+ cells relative to the CD38− population
(cluster 1, Fig. 5a). HOXB genes were also downregulated during the transition of the
CD38− HSC-MPP to the CD10−CD62Lhi LMPP stage with no significant further change at
the CD10+ stage (cluster 1). In contrast, expression of HOXA genes decreased later in
differentiation at the CD10+ progenitor stage (cluster 2 and 3). Reciprocal patterns of
expression were seen for members of the polycomb repressive complexes PRC1 (PCGF2,
PHC2, and SCML4) (cluster 1) and PRC2 (SUZ12, EZH2 and EED) (cluster 5)24. These
analyses reveal a highly coordinated program of transcriptional regulation as HSC lose
multipotency, become lymphoid-primed and then commit to B lymphopoiesis.
Lymphoid differentiation stages of CD10-CD62Lhi and CD10+ cells
Analyses of genes up-regulated only in the CD10− CD62Lhi population (Cluster 4) revealed
a profile consistent with the dual lymphoid and monocyte potential of this population.
Specifically, early T and NK lineage-associated genes (CD2 and CD3E)14,25–27 and
lymphoid cytokine receptors (IL2RG, IL10RA, IL10RB, IL17RA, IFNGR1) were up-
regulated, as were myeloid associated genes (MPO, CSF1R, and CSF2R) (Fig 5b, c).
Consistent with its cell surface expression, FLT3 was expressed in both HSC-MPP and
CD10− CD62Lhi cells but not CD10+ cells (cluster 3, Fig 5b).
Consistent with the B cell skewed differentiation potential of the CD10+ population, genes
known to be expressed specifically during B cell commitment (EBF1, PAX5, IL-7R,
CD79A, CD79B, VPREB1, VPREB3, CD19, CD22, CD24, CD27) were highly expressed
Kohn et al.
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text:
County News
Fairfield, Delaware and Stark County Farm Bureaus
Reporters, please visit our news room located in the Media and Publications section of this site.
Fairfield, Delaware and Stark County Farm Bureaus
Accidents can happen to the safest drivers. And when they do, safe drivers auto insurance rates can rise by as much as 30 percent. Plus, accidents and violations could affect your insurance rates for up to three years.
Ron Warnock, volunteer and Betsy Anderson, staff
Here are some additional activities in which your support of Farm Bureau helped strengthen agriculture and rural Ohio.
Grassroots program now featured in Buckeye Farm News
Two state issues that voters Nov. 3 will be deciding deal with compensation for military veterans and casinos in four Ohio cities.
Five farm families were honored for their long-standing dedication to natural resource conservation during ceremonies at the Farm Science Review.
David Moroschan of Seneca County recently received Ohio Farm Bureaus annual Excellence in Crop Advising Award.
Broad support shown for Livestock Care
Dr. Gee gave Ohio Farm Bureau his opinion on Issue 2 during recording of its Town Hall Ohio radio program. Here's what he had to say.
Ohio Farm Bureau speaks out at USDA hearing
Ohio State University President E. Gordon Gee and OSUs new Dean of the College of Veterinary Medicine Lonnie King are this weeks special guests on Town Hall Ohio.
Retail food prices at the supermarket decreased slightly for the fourth consecutive quarter and are significantly lower than one year ago, according to the latest American Farm Bureau Federation Marketbasket Survey.
Ohio Farm Bureau's Jack Fisher, HSUS's Paul Shapiro to debate State Issue 2 live on Cleveland radio station. Listeners calls will be taken.
Out of state animal rights activists say Issue 2 is about big ag and not family farms. With nearly 60,000 family farmers in its membership, Ohio Farm Bureau knows this isnt true. Meet some multigenerational family farmers who agree.
The Ohio Farm Bureau Federation recognizes the challenges facing Gov. Strickland and the Ohio General Assembly
Changes to the Renewable Fuel Standard proposed by the Environmental Protection Agency (EPA) would impede the growth of the U.S. biofuels industry, limit opportunity for American farmers and ranchers to grow their businesses and hinder American energy independence, according to the American Farm Bureau Federation.
Ad campaign, Web site, rallies call for YES vote for Issue 2
Farm income & expenses forecast, broadband funds, new commission, pork support, value-added grants, new Web site for beginning farmers and country of origin labels.
Some rural landowners may be surprised when discovering their latest CAUV (Current Agricultural Use Valuation) values have increased some several hundred percent over the last three years.
Saying that it could reduce net farm income by more than $5 billion over the next 10 years, Ohio Farm Bureau Federation (OFBF) President Brent Porteus recently told a panel of lawmakers that farmers were concerned about cap and trade legislation passed by the U.S. House.
Now is the time for all farmers, regardless of what they produce, to come together in a unified effort to protect Ohio agriculture,
Congress may take action this fall on three bills of critical importance to farmers. According to the American Farm Bureau, here is what you should know.
Its not quite E-harmony.com, but a new Web site is hoping to match farmers and hunters in four Ohio counties.
Updates from Mercer, Delaware and Wayne County Farm Bureaus.


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Cole Stoudt has some big shoes to fill in Clemson replacing Tajh Boyd under center. If he can stay as poised under pressure as he did pretending to be a mannequin, he'll have no trouble.
To show off new equipment and uniforms, Clemson has setup up a display of a few mannequins at the WestZone at Memorial Stadium. At some point, someone realized that in full gear, Stoudt looks like a mannequin. This gave him an opportunity to surprise a few unsuspecting players, employees and even a few kids.
The results are fantastic and the reactions are probably similar to how defensive backs felt last season watching Sammy Watkins come at them. Check out the video:
[RELATED: CSN's Preseason Top 25]


text:
15 Actors Who Made Extreme Weight Changes for Movies
Top Deadliest And Most Intimidating DC Villains of All Time
Where Are They Now? The Cast of 'Willy Wonka and The Chocolate Factory'
The Horror Games You Have to Play This Year
Kaley Cuoco Shows Off Her Tennis Skills
This One Good Idea in Terminator Genisys Would Make a Great Sequel
Is Taylor Swift Joining the Cast of 'X-Men'?
The Cameraman Captured More Than Expected
'Real' Disney Men We Would Totally Date
Movies You Didn't Know Were Blatant Rip-Offs
20 Movies You Need To Watch At Home This Week
The New Lady Ghostbusters Are Ready To Kick Butt!
10 Celebrities Who Quit Hollywood Forever
11 Celebrities That Married One Of Their Fans
Tom Cruise Pitches Edge of Tomorrow Sequel Concept
A New Batman and New Batmobile for 2016 Movie
First Look At Leaked Warcraft Movie Trailer
10 Actors Who Played a Movie Character Until They Died
10 Movies to Watch After You See Straight Outta Compton
The 10 Most Promising TV Shows of Fall 2015
Will Christian Bale Ever Play Batman Again?
8 Historical Facts That Will Change Your Concept of Time Forever
6 TV Finales That Failed Spectacularly
Ridley Scott on Why Prometheus 2 is Now Alien: Paradise Lost
The Best and Worst Marvel Villains
Grant Gustin Teases 'Big Twist' at End of The Flash's First Season
Fear the Walking Dead Characters You Should Know
Game of Thrones Spoilers: Season 6 Parade of Lies
6 Films That Prove a Great Fantastic Four Movie is Possible
'Saved By The Bell' Star Tiffani Thiessen Makes J.J. Watt's Day


text:
London—Victoria Beckham, who reportedly has amassed an "it" handbag collection worth more than $2 million, is the latest celebrity planning her own handbag collection.
Beckham–wife of soccer superstar David Beckham and the Spice Girl known as "Posh" for her extravagant fashion tastes—is expanding her fashion empire with a handbag collection of her own with design assistance from Katie Hillier, the accessories designer who has created signature bags for Marc Jacobs, Luella and Stella McCartney.
A spokesperson for Beckham confirmed Monday: "There is a bag line in development. Katie has consulted on the technical aspects of bag construction in line with her experience in the industry."
But make no mistake about it, says Posh's spokesperson: "This collection is not a collaboration. As with all her ranges, Victoria has sought the best consultants to ensure the highest level of quality at manufacturer for her designs."
Longtime 'It' Bag Lover
While specific launch date has been yet to be revealed, British tabloid reports speculate that Beckham decided to do her own handbag line since whatever handbags and accessories she wears get so much attention. Beckham, who reportedly owns 100 Hermes Birkin bags, is supposedly fed up with helping other designers' accessories sales increase because she wears their designs. One of the most photographed women in the world, Beckham has been shot carrying Birkins in every color and material as well as other designer "it" bags.
Earlier this year, British tabloids poked fun at the 36-year-old mother and business entrepreneur's fondness for her handbags, even reporting that she employs a personal assistant just to tote around her handbag when she's traveling or shopping.
Last week, La Beckham supposedly tapped her husband to help design a new menswear line, but her company denied the rumor.
For commercial purposes, extract supplements can be obtained from the fruit of the pericarp. Garcinia cambogia reviews is performed by a physician Chen and Oz to prove that it is an herb for weight loss, a lot of research has been done. Studies, Garcinia cambogia extract supplementation decreases the weight of the body fat accumulation, was shown to have a positive effect on prevention. We are important substances such as cholesterol (LDL), reduces the serum leptin and triglycerides. In addition, it will increase the level of serotonin and cholesterol (HDL). The study also, Garcinia cambogia extract supplements, has proven that there are no significant adverse effects on many of the eight weeks, such as its use.


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Look back at the action as it unfolded
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text:
Analysis: Pay It Forward
Inexact title. See the list below. We don't have an article named Analysis/PayItForward, exactly. We do have:
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If you meant one of those, just click and go. If you want to start a Analysis/PayItForward page, just click the edit button above. Be careful, though, the only things that go in the Main namespace are tropes. Don't put in redirects for shows, books, etc.. Use the right namespace for those.


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After years of sitting through way too many banal, sleep inducing PowerPoint presentations, I finally could not take it any more and dashed off this missive which I originally wrote for my students. I believe there are seven crucial presentation design habits that will help you develop more engaging … [Read more...]
10 reasons why making documentaries is better than making fiction films
Peter Broderick, who helps filmmakers "explore uncharted frontiers and avoid dangerous pitfalls and predators," gave a wonderful talk this morning at Sheffield Doc/Fest, one of the many gems in his presentation was his list of ten reasons why making documentaries is better than making fiction … [Read more...]
Making Media Now 2012: Thriving in a Changing Landscape
The Making Media Now conference organized by Filmmakers Collaborative has become the annual must-attend event for New England media makers. This year the event will mark Filmmaker's Collaborative's 25th anniversary and takes place on Friday, June 8, 2012 at Massachusetts College of Art and Design … [Read more...]
Researching Macro Trends
While I was preparing my presentation, "Seven Macro Trends," I reached out to people I thought might have some ideas and/or examples I should weave into my presentation. I'm indebted to their wonderful and generous contributions. What follows are the highlights of their responses to my query, "what … [Read more...]
Seven Macro Trends (RioSeminars 2011 Presentation)
I promised during my keynote presentation on Monday, October 10, 2011 at RioSeminars 2011 that I would post my slides and some notes before Sunday at midnight, so here it is: 7 Macro Trends, RioSeminars 2011 (5 MB, PDF), and below are some notes that go with the slides. One reason I find it … [Read more...]
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text:
Sister Leather Bracelet, Filigree
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Product Description
Our collection includes exquisite angels, cherubs, gift plaques, candles, and intricate jewelry accessories.
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Product Information
|Format: Gift
Vendor: Pavilion Gift
Publication Date: 2010
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Product Reviews
ElainaVernon, BCAge: 18-24Gender: female4 Stars Out Of 5niceJune 30, 2012ElainaVernon, BCAge: 18-24Gender: femaleQuality: 4Value: 4Meets Expectations: 4It is a leather, and thats what i like about it the most. I bought it for my sister, she liked it...:)


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(623) 628-5558
14955 W Bell Rd, 8526, Surprise, AZ 85374 map »
------->FREE DOOR TUNE UP with every repair. *VALLEYWIDE SERVICE!* Over 21 years experience. Certified technicians. SAVE BIG $$$ with our Kudzu coupons. 5 STAR REVIEWS! We service all makes and models. Click on our reviews and see why people have chosen Bullfrog's for their garage door needs.
We look forward to providing you with our fast services. Thank you for choosing Bullfrog's.
We strive to be the leading garage door company in the.. valley. We are family owned and operated serving the valley since 1993. Licensed, Bonded & Insured. We're dedicated to providing high quality customer service and workmanship. At Bullfrog's we believe every customer is extremely valuable to our company and customer satisfaction is our main goal. We specialize in residential garage doors and garage door openers, repairs, sales, and service. We use top quality parts all at a price that is designed to work within your budget. Visit our website at www.bullfrogdoors.com to see a wide variety of doors. You can also visit our showroom or schedule for our FREE in home estimate (with purchase of a new door) and we can help you decide which door or electric opener will best fit your needs.
To all of our customers who took the time to give us such great reviews. WE THANK YOU!!!!!!!
2012, 2014
Licensed, Bonded & Insured AZROC 225070-C05 GMI Select Factory Certified Technician. "Genie Chain drive & Screw drive Model". Members of the BBB and PORA homeowners association. We are an EPA Lead-Safe Certified Firm
Over 18 years experience. We stay up to date with training seminars and workshops to keep us current with all the latest innovative technology. We only use professional quality parts built to last!
* By Appointment Only
[JPG] Have your door serviced regularly so this doesn?t happen to you!
[JPG] Give your house a whole new look!
[PDF] Choose which LiftMaster opener will work best for you.
[JPG] Steel doors with faux paint - look like wood!
[JPG] We can do sections only.
We offer Insulated garage doors that can reduce the cost of your electricity bill. We also help the environment by recycling all of the old doors and material that we remove. Thank you for visiting Bullfrogs Garage Door Company.
Customer service is outstanding! Prices very reasonable! All-in-all if your garage door breaks, this is the company to call! I will definitely recommend them to others...Already have!
My garage door springs broke (both) and they got here that same day, fixed fast and at a fair price! I highly recommend Bullfrog!!
Displaying 1-15 of 289
Customer service is outstanding! Prices very reasonable! All-in-all if your garage door breaks, this is the company to call! I will definitely recommend them to others...Already have!
Reviews: 1
Posted on: 2/10/2014
My garage door springs broke (both) and they got here that same day, fixed fast and at a fair price! I highly recommend Bullfrog!!
On top of being very friendly and taking his time to install correctly the first time, Jeremy did a superior job verifying what we wanted for our garage door openers and the compatibility for our car's HomeLink connection. He did a very clean, neat and professional installation, was very conscientious and careful of his work and was very thorough w/ making sure we knew how it operated. And Heather's scheduling arrangements and appointment follow-up were yet further demonstrations of professionalism that is much appreciated. We are very pleased w/ the complete ordering, scheduling and installation process from start to finish!
Had a spring pop on Tuesday morning. Left them a message at 7 a.m. and got a call back by 7:15 a.m. Sign of good things to come. We were not going to be home due to work obligations, but Heather had all the solutions for us. She had someone out by late morning to replace both springs and add a wireless remote for us. They contacted us upon arrival and gave a very fair quote before starting the work. Secured the garage and side gate upon leaving. Excellent experience all around, we will definitely use them in the future and refer them to everyone we know!
Really happy with the service I received from Jeremy and Bullfrog's Garage Doors! Easy to schedule, showed up on time, excellent service and fair prices. I'll use them any time I need garage door work....and am happy to recommend them to others!!
After reading several positive reviews. I contacted Bullfrog to repair my 10 year old garage door that would not fully open. The receptionist was very pleasant and professional. Jeremy contacted me 30 min prior to arriving as requested. He fully inspected the springs, rollers and hinges along with the opener. He does not use high pressure sales or scare tactics. He provided professional service at a reasonable price. I opted to replace the springs because they were 10 years old. He completed the job in approx. 1 hour. Thanks Jeremy I wish you and your company continued success. Bob
Thank you Jeremy for responding to our Garage door issue with great service and an affordable price. We will highly recommend Bullfrog to all our friends and neighbors.
Reviews: 2
Posted on: 4/17/2012
Great experience overall. On time & efficient in completing the job. Fair price with no pressured upsell. Would highly recommend Bullfrog!
Honest and Dependable! With so many garage door people to choose from I decided to read this guys reviews and was not disappointed. I called the night before and he called me back at 7:20am and was here repairing it by 8:00am. He even called before coming out so I could pull the cars out and have it ready for him. Jeremy and his helper were very friendly and knowledgable and got to work right away. I had a spring break and decided to replace them both. He let me know that since I had insulated my door I could use a heavier duty spring and that is what he replaced it with. He also let me know my rollers were worn out but didn't try to sell me anything and he even did a tune up and greased everything which it needed. He was honest with me and so I had those replaced to and now my garage is quieter and works like new. I will be recommending them to my friends and family as an honest garage door man who does excellent work and I now have a garage man I can use, who I trust when I need him,
Called in late morning, they were able to repair my door before the afternoon was over. Clearly explained what was needed and I thought the price was fair.
It is always a gamble trying to find a good quality company so I feel very fortunate to have found Bullfrog's. They tell you only what you need and you get exactly what they promise. They are professional, courteous and just plain quality at a quality price.
I've dealt with Bullfrog's Garage Door Inc since 2007. They have done repair work, installed a door opener, done maintenance and yesterday they bailed me out again when the garage door jammed. I called Jeremy and as always he was friendly, professional, quick to arrive and the job was completed in a timely fashion and cost alot less than what I thought it would. Bullfrog's Garage Dorr Inc. is definitely a company I recommend. Oh, and thanks Jeremy for the "lift"! You are the best!!!!
First of all thanks to all the reviews for assisting in using Bullfrog. Second I was very impressed with the job and service Jeremy provided. I bumped back into our garage door and damaged 2 of our panels. (Yep I did). Jeremy was quick to return my call, answered all questions and was quick with repair. He explained everything in detail! High recommend!! Thank you.
Reviews: 1
Posted on: 12/21/2011
I have been having ongoing garage door issues for about a year now. I did a lot of research on garage doors & garage door companies. After finding a local company with such great reiews I knew I had to try them. I was NOT disappinted! I don't think I've ever had a company call with an issues, show up on time, explain everything and do such a fantastic job as Jeremy and his partner did. I will refer them to all of my friends and family. Thank you!
Just bought a bran new spec home in far east Mesa. Our new neighbors highly recommended this company. Prior to installation, Jeremy would always answer the phone with last minute questions. He showed up on time, and went up and beyond my expectations with professionalism and kindness. A true diamond in the rough! You will not regret choosing this company.


text:
As unemployment continues to be a major issue across the country, employees are more willing than ever to pull up the tent stakes and move across the state or even across the country for new jobs. Employers often move existing employees to new locations in order to maintain an experienced and trained workforce. A recent study of member companies by Worldwide ERC indicated that the average cost of moving a home-owning employee is approximately $69,000 for a new hire and $90,000 for an existing employee. Many employers pay all or a part of the costs, but employees often have to shoulder significant expenses in order to move for a job. Here are five issues to consider before you agree to relocate.
SEE: 8 Career Risks That Pay Off
Industry Isolation
Thinking about losing your new job is likely not high on your list of considerations, but it should be. Before deciding to make the move, research the other companies in the new city that hire employees with your skill set. If there are very few, you may find yourself stuck in an area where you won't be able to find a new job in your field without moving again.
SEE: 5 Ways To Be Irreplaceable At Work
Real Estate Market
Since the recession of 2008, real estate sales have stalled and home values have declined. It may not be as easy as it once was to quickly sell your old house in order to buy a new one. Banks are less likely to extend bridge loans to allow you to purchase before you sell, as the credit market continues to be tight. On top of that, if you are moving from an area with depressed real estate prices to a booming area, you may lose some of the existing equity in your home.
Employer Incentives
Find out exactly how much your employer is prepared to pay for the move, either in direct costs or a moving bonus. Before accepting, outline all of the costs of the move and ensure that the employer's contributions cover a significant portion of those costs. Even if your new job pays more, it won't benefit you until you recover the out-of-pocket costs of the move.
SEE: How Moving Can Affect Your Finances
You won't be working 24 hours a day, so the type of community you are moving to makes a difference. If you prefer the quiet of the suburbs, moving into a condominium in Lower Manhattan can make your non-working hours unbearable. This is even more important if you are moving a spouse and children with you. Are there activities for kids, green space for walking and recreational facilities, such as movie theaters and concert halls? What are the local schools like? Check out the new city before moving to ensure that it fits with your lifestyle.
Upward Mobility
Your future with any company isn't a slam dunk, but if you move in order to start a new job, consider the future opportunities to move up. For example, if your current employer wants to move you out to Boise, Idaho to manage the plant, reflect on how that fits in with your goals to be a corporate vice-president five years down the road. Ensure that your career path is aligned with the potential to grow at the new location.
SEE: 3 Ways To Attract Job Opportunities
The Bottom Line
Packing up your life and moving for a new job is a major event, both professionally and financially. Upfront planning and analysis can help keep you from jumping into a situation that can hurt your wallet or your future career track. Be sure that the move benefits you as much as it benefits the employer.
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In the air tonight
I made another visit to Sparks lake in Central Oregon this evening, and got another decent sunset at this place. There were a lot of clouds when a friend and I arrived at the lake, but as the sun was setting lower, the clouds started vanishing. Luckily, there were still a few clouds left by the time the sun was at the lowest angle. This is one of the compositions that I captured...
8-second exposure, 19mm @ f/9, ISO 50, B&W circular polarizer, 3-stop Lee GND filter.
21,905 views
43 faves
17 comments
Taken on August 30, 2011


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Excellent staff. Very friendly and welcoming
Great place, friendly staff. Nice room and overall accommodations. The included breakfast was also varied and tasty. A fantastic price to quality ratio !
This was a central location, within easy walking distance to the small downtown area.
The pictures when booking all focused on the main building - a historical, sophisticated-looking inn. However, when we arrived we discovered that we were placed in the motel out back, NOT what we had been expecting or wanted. We had been hoping for something more upscale, to say the least. The view out of the small windows was of a narrow alley with trash, rather than the beautiful Green Mountains we had hoped to see. This would have just been a minor inconvenience (for the price, we probably should have expected a less-than-stellar place), but the beds were also very "well-used" (i.e. sunken into the middle), which caused us both to sleep horribly. My husband ended up with a very sore back the next day, putting a damper on our exploration of the area and resulting in a visit to the chiropractor when we returned home. We will NOT be returning to this "inn" for our future visits to Vermont.
It is old but that helps with the charm. Clean and well maintained
Friendly staff, breakfast, location.
Motorcycle noise.
Great staff and comfortable.
A bit noisy because the floors are linoleum rather than carpet. Late night noises were very distinguishable.
The king sized bed was very, very comfortable. That's what it's all about for me. I was so glad to have a good rest.
Clean, pleasant staff
Bistro food options could have been better, fresher baked goods
I ordered a rollaway bed and for the first time EVER in my experience of traveling (and I am a former flight attendant so I've traveled a lot) it was actually in the room when I arrived. I didn't have to ask to have one sent up! Thank you, Marriott!
Breakfast was not included in the price of the overnight stay.
It was so beautiful and the food was excellent. Breakfast and dinner both were fantastic. The room was great, everyone was so friendly and accommodating. We had a wonderful time and would love to go back.
The inn was very comfortable and nicely decorated. Staff were extremely friendly and eager to please. The food in the restaurant was delicious.
Nothing in particular.
This hotel should not be servicing people.
Location and cost
Bathroom seemed dirty. No hair dryer. Loud road noise. No ice machine you could get to (had to get office staff to get it for you). Breakfast very poor.
Here's why:
We're not the seller
At KAYAK we don't set prices, so it's not possible for us to guarantee what other companies are selling.
We aggregate tons of data for you
Our service lets you quickly and easily compare results from hundreds of travel sites at once. In this sense, KAYAK is a search engine and we don't guarantee prices we find.
Why aren't prices accurate 100% of the time?
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|ISSN 1088-6850(online) ISSN 0002-9947(print)
The slice map problem for -weakly closed subspaces of von Neumann algebras
Abstract | References | Similar Articles | Additional Information
Abstract: A -weakly closed subspace of is said to have Property if for any -weakly closed subspace of a von Neumann algebra , where is the right slice map associated with . It is shown that semidiscrete von Neumann algebras have Property , and various stability properties of the class of -weakly closed subspaces with Property are established. It is also shown that if is a -dynamical system such that has Property and is compact abelian, then all of the spectral subspaces associated with have Property . Some applications of these results to the study of tensor products of spectral subspaces and tensor products of reflexive algebras are given. In particular, it is shown that if is a commutative subspace lattice with totally atomic core, and is an arbitrary subspace lattice, then .
Retrieve articles in Transactions of the American Mathematical Society with MSC: 46L10, 46L55, 46M05, 47D25
Retrieve articles in all journals with MSC: 46L10, 46L55, 46M05, 47D25
Additional Information


text:
Good afternoon, and welcome to Styleite. If you're just joining us, then you probably haven't noticed yet that we really hate Photoshop.
Let us rephrase — we detest the overuse of Photoshop to alter the appearance of already beautiful people to make them fit a standard of beauty only achievable through digital photo manipulation. And in its new (and first) campaign, Proenza Schouler's advertising people seem to have done just that.
People have made jokes about models being so thin they disappear in profile, but the nonexistent waist of the model in this ad is no laughing matter. In fact, unless she's had several major organs removed, it's an anatomical impossibility. The 18-inch waist of Ann from the new cycle of ANTM notwithstanding, no amount of posing or styling or lighting could explain this one to us. Because if her waist were as small as the people who designed this ad would like for us to believe it is, her legs and thighs suggest that she has real hips — yet those, miraculously, have disappeared as well.
We always admired Jack McCollough and Lazaro Hernandez because they represented to us what hard work can accomplish. They are truly talented and visionary designers who make clothes we can see real people wearing. But the model in this photo is not in any way representative of reality, and that's something we just won't stand for.
[H/T to HuffPo Style]


text:
Singers: The San Diego District is no longer accepting applications for their 2015 MONC auditions.
Check out our Judges page
To learn how you can help, learn about our Patron Program.
If you would like to be added to our mailing list, or if you would like to be a volunteer for the auditions Contact Us and send us your name, street address, phone number and email address.


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you are viewing a single comment's thread.
Yeah, but the hit is only on T, not all telecoms.Why?
because weak hands listen the tardbutts on CNBC talk about divi cuts if Obama blah blah blah...the same clowns promote Apple,,whothefck do they think gets all the repeat data revenue?? Mobile data movement is so huge its beyond their peabrain mental capacities!!!!
meant to say " divi taxes " ..sorry...same blowhard MM CNBC hedge buddies have been sayin that since T was at 27$...tards


text:
I was entertained with my favorite show. I will miss it. Whenever I see Petersen I think gruesome Grissom and smile. The show had "real life" people with their quirks, humor, etc. It had real plots to it instead of the "write a few words, insert cuss words here, and make everything about sex every other paragraph.
My favorite episode was the guy that got shot, he was bit by a rattle snake, drowned etc. I intend to watch it again. I am close to dumping TV and going with all access cbs. CBS creates programming that is entertaining, not insulting and good plots. CBS respects their viewers.


text:
er to nurse a seriously ill neighbor.
"I may be back tomorrer and I may not be back till the day after never! I declare I'm all of a fluster, what with Mis' Calvert goin' away sort of leavin' me in charge--though them old colored folks o' her'n didn't like that none too well!--and me havin' to turn my back on duty this way. But sickness don't wait for time nor tide and typhoid's got to be tended mighty sharp; and I couldn't nohow refuse to go to one Mis' Judge Satterlee's nieces, she that's been as friendly with me as if I was a regular 'ristocratic like herself. No, when a body's earned a repitation for fetchin' folks through typhoid you got to live up to it. Sorry, Dolly C.; but I'll stow the girls, Barry and Clarry and the rest, 'round amongst the neighbors somewhere, 'fore I start. As for you, Alfy----"
"Oh, Mrs. Babcock! Don't take Alfy away! Please, please don't!" cried Dorothy, fairly clutching at the matron's flying skirts, already disappearing through the doorway.
Mrs. Babcock sw
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FRANCE: Pernod board declares dividend
Pernod Ricard's board of directors agreed on 18 December 2003 to pay an interim dividend of € 0.90 per share with a € 0.45 tax credit for retail investors. This interim dividend will be paid on 13 January 2004.
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NonBlockingHashMap seems to have unbounded growth when using unique keys, even if the actual set of entries is small.
This gist illustrates the problem: https://gist.github.com/3568485
It simply inserts and removes a monotonically increasing integer. I.e., the working set is at most one object, but the map still grows to use all available heap, at which point it will eventually die with a OutOfMemoryError.
This problem has been observed in the wild in Cassandra, where NonBlockingHashMap is used liberally.
Daniel Norberg
Jonathan Ellis
This is another manifestation of the behavior I observed in http://sourceforge.net/tracker/?func=detail&aid=2828100&group_id=194172&atid=948362 and http://sourceforge.net/tracker/?func=detail&aid=2835696&group_id=194172&atid=948362: remove replaces the value with a tombstone, but the (key, tombstone) entry will not be cleared until the map resizes. So if you keep inserting unique keys you will indeed grow the map until it OOMs before the next resize.


text:
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From Wikitravel
Shanghai : Changning
Changning is a district in Shanghai frequented for the Hongqiao Airport and Shanghai Zoo.
Get in[edit]
Zhongshan Park Metro station forms the center of this district of Shanghai.
See[edit][add listing]
Do[edit][add listing]
Buy[edit][add listing]
Brilliance West Mall is at the corner of Xianxia lu and Jianhe lu. There are many clothing shops and restaurants as well as a home furnishings store, Hola, below the mall. There is a Watsons and a Decathlon sports equipment store with western side facing Jianhe Lu.
Cloud Nine shopping center at Zhongshan Park has just about everything. Worth a look.
Eat[edit][add listing]
Several restaurants are available at Brilliance West Mall. You can also do grocery and household shopping at Century Mart.
Drink[edit][add listing]
Sleep[edit][add listing]
Mid range[edit]


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If you've been wondering why Slate has been so terrible recently, so Upworthy and Buzzfeedy, it's a good bet the cause is the smothering presence of neoliberal turd Matt Yglesias. A twitterati with no degree in economics, he regularly suggests the answer might be somewhere in the middle and gives a thorough tongue bath to big business. He writes 3-4 articles a day, so there are a lot of bad ones, but I have picked out some of my favorites to celebrate from 2013.
Premise: A man who never leaves a city and calls himself a liberal finally gets to go inside a Walmart and he loves it. Turns out liberals were wrong. Walmarts are great. You can buy all sorts of things. Before the article ends he contradicts himself and decides that Walmarts are doomed in the age of Amazon.
Premise: This article repeats about once a month on Slate, with good reason. If you had to summarize the ideology Yglesias tries to communicate as a writer (he is no more a journalist than Fark's approval queue) it is that Amazon Is The World's Most Amazing Company.
Finally: an advice column for the rest of us! (Who seek to enslave humanity.)
Even the most fervent gun control advocate would admit that guns kill people, not other guns. We must become the guns we wish to see in the world.
In the latest Metal Gear twist, Big Boss could be ANYONE - politicians, celebrities, dogs, even YOU!
A Goon outs himself as a wizard and gets a warm reception from the rogues/rangers/paladins gallery!
Malcolm McDowell, Lou Gossett Jr., cocaine-fueled Ewok traps, and…a nuclear Y2K holocaust?
Featured articles and columns that don't fit anywhere else on Something Awful.
Copyright ©2015 Rich "Lowtax" Kyanka & Something Awful LLC.