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Publications (28)139.99 Total impact
Article: Relationship Between Genital Drug Concentrations and Cervical Cellular Immune Activation and Reconstitution in HIV-1-Infected Women on a Raltegravir Versus a Boosted Atazanavir Regimen[Show abstract] [Hide abstract]
ABSTRACT: Determinants of HIV-infected women's genital tract mucosal immune health are not well understood. Because raltegravir (RAL) achieves relatively higher genital tract concentrations than ritonavir-boosted atazanavir (ATV), we examined whether a RAL-based regimen is associated with improved cervical immune reconstitution and less activation in HIV+ women compared to an ATV-based regimen. Peripheral blood, cervical brushings, cervical-vaginal lavage (CVL), and cervical biopsies were collected from HIV+ women on TDF/FTC and either RAL (n=14) or ATV (n=19) with CD4+ T-cells >300 cells/mm3 and HIV RNA <48 copies/mL. HLA-DR+CD38+ T-cells were measured in blood and cervical cells using flow cytometry, CD4+ and CD8+ T-cells were quantified in cervical biopsies by immunofluorescent analysis, and HIV RNA (VL), ATV, and RAL concentrations were measured in CVL. In a linear regression model of log(CVL concentration) versus both log(plasma concentration) and treatment group, RAL CVL level was 519% (95%CI: 133, 1525%) higher than for ATV (p<0.001). Genital tract VL was undetectable in 90% of subjects and did not differ by regimen. There were no significant differences between groups in terms of cervical %HLA-DR+CD38+CD4+ or CD8+ T-cells, CD4+ or CD8+ T-cells/mm2, or CD4:CD8 ratio. After adjusting for treatment time and group, CVL:plasma drug ratio was not associated with cervical CD4:CD8 ratio or immune activation (p>0.6). Despite significantly higher genital tract penetration of RAL compared to ATV, there were no significant differences in cervical immune activation or reconstitution between women on these regimens, suggesting both drug regimens achieve adequate genital tract levels to suppress virus replication.AIDS research and human retroviruses 06/2015; DOI:10.1089/aid.2014.0301 · 2.33 Impact Factor
Article: Reduced Immune Activation During Tenofovir–Emtricitabine Therapy in HIV-Negative Individuals[Show abstract] [Hide abstract]
ABSTRACT: Elevated immune activation is associated with an increased risk of HIV acquisition. Tenofovir (TFV) has immunomodulatory properties in vitro, but how this extends in vivo remains unknown. HIV-negative adults received daily coformulated TFV disoproxil fumarate 300 mg/emtricitabine (FTC) 200 mg for 30 days followed by a 30-day washout. Markers of T-cell activation, inflammation, and cytokines were measured before drug and on days 30 (on drug) and 60 (30-day washout). Data were analyzed using one-way analysis of variance/pairwise comparisons. Intracellular disposition of TFV-diphosphate and FTC-triphosphate in CD4 and CD8 T-cells and monocytes was characterized, and the relationship with immune activation was evaluated using Pearson's correlation coefficient. T-cell activation was available in 19 participants. CD38/HLA-DR coexpression on CD8 T-cells decreased from baseline to day 30 (3.97% vs. 2.71%; P = 0.03) and day 60 (3.97% vs. 2.41%; P = 0.008). Soluble CD27 decreased from baseline to day 60 (184.1 vs. 168.4 pg/mL; P = 0.001). Cytokines and inflammation markers were not significantly different. TFV-diphosphate and FTC-triphosphate were approximately 4-fold higher in monocytes vs. CD4 and CD8 T-cells but neither correlated with activation markers. TFV disoproxil fumarate/FTC therapy was associated with decreased T-cell activation in HIV-negative adults, which could contribute to the antiviral effect of pre-exposure prophylaxis (NCT01040091; www.clinicaltrials.gov).JAIDS Journal of Acquired Immune Deficiency Syndromes 04/2015; 68(5):1. DOI:10.1097/QAI.0000000000000529 · 4.56 Impact Factor
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ABSTRACT: Background. This study estimated the number of daily tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) doses required to achieve and maintain (after discontinuation) intracellular drug concentrations that protect against human immunodeficiency virus (HIV) infection for men who have sex with men (MSM). Methods. Tenofovir diphosphate (TFV-DP) concentrations in peripheral blood mononuclear cells (PBMCs) and rectal mononuclear cells from an intensive pharmacokinetic study ("Cell-PrEP" [preexposure prophylaxis]) of 30 days of daily TDF/FTC followed by 30 days off drug were evaluated. A regression formula for HIV risk reduction derived from PBMCs collected in the preexposure prophylaxis initiative study was used to calculate inferred risk reduction. The time required to reach steady state for TFV-DP in rectal mononuclear cells was also determined. Results. Twenty-one HIV-uninfected adults participated in Cell-PrEP. The inferred HIV risk reduction, based on PBMC TFV-DP concentration, reached 99% (95% confidence interval [CI], 69%–100%) after 5 daily doses, and remained >90% for 7 days after stopping drug from steady-state conditions. The proportion of participants reaching the 90% effective concentration (EC90) was 77% after 5 doses and 89% after 7 doses. The percentage of steady state for natural log [TFV-DP] in rectal mononuclear cells was 88% (95% CI, 66%–94%) after 5 doses and 94% (95% CI, 78%–98%) after 7 doses. Conclusions. High PrEP activity for MSM was achieved by approximately 1 week of daily dosing. Although effective intracellular drug concentrations persist for several days after stopping PrEP, a reasonable recommendation is to continue PrEP dosing for 4 weeks after the last potential HIV exposure, similar to recommendations for postexposure prophylaxis.Clinical Infectious Diseases 11/2014; DOI:10.1093/cid/ciu916 · 8.89 Impact Factor
Article: Short Communication: Tenofovir Diphosphate in Dried Blood Spots As an Objective Measure of Adherence in HIV-Infected Women[Show abstract] [Hide abstract]
ABSTRACT: Simple and reproducible tools to assess antiretroviral adherence are needed. A level of tenofovir diphosphate (TFV-DP) in dried blood spots (DBS) <1250 fmol/punch is predicted to identify imperfect adherence. Herein we evaluated TFV-DP DBS as a measure of adherence among HIV-infected women. DBS and peripheral blood mononuclear cells (PBMCs) were collected twice (~1 week apart), in 35 well-controlled HIV-infected women (median age 42 years, 14 African American/Black [AA]) receiving daily co-formulated tenofovir/emtricitabine and either atazanavir/ritonavir (n=20) or raltegravir (n=16). TFV-DP in DBS and PBMCs was quantified by LC-MS/MS. Six-month adherence was measured as average days between monthly pharmacy refills. Data were loge transformed for analysis and presented as median (range); the correlation between continuous variables was analyzed using Pearson correlation coefficient. The average TFV-DP between the 2 visits (aTFV-DP) in DBS and PBMCs was 1874 (706-3776) fmol/punch and 125 (1-278) fmol/106cells, respectively. AA women had lower levels of aTFV-DP in DBS compared to whites (1660 vs. 1970 fmol/punch; P=0.04), with a viremic patient having the lowest drug level (706 fmol/punch). Days between pharmacy refills were 34 (30-54) vs. 30 (26-40) in women with TFV-DP in DBS <1250 vs. ≥1250 fmol/punch (P=0.006). TFV-DP in DBS was negatively correlated with increasing number of days between refills (r=-0.56, P=0.002). TFV-DP DBS was a reliable and objective measure of adherence in HIV-infected women based on a strong inverse relationship with pharmacy refill adherence.AIDS Research and Human Retroviruses 10/2014; 31(4). DOI:10.1089/AID.2014.0229 · 2.33 Impact Factor
Article: Safety of Excisional Inguinal Lymph Node Biopsies Performed for Research Purposes in HIV-1-Infected Women and Men[Show abstract] [Hide abstract]
ABSTRACT: Background: Most HIV-1 replication occurs in secondary lymphoid tissues, and evaluating these tissues is crucial to investigations of pathogenesis. Inguinal lymph nodes (LN) are obtained frequently for these studies as they are readily detectable in most individuals and provide abundant numbers of cells. Knowledge of the outcomes of inguinal LN excision for research purposes is important to inform accurately study participants and researchers of the potential risks. Methods: Data on surgical complications were collected in real time in HIV-1-infected subjects who underwent excisional inguinal LN biopsies for research purposes from February 1997 through June 2011. Data were analyzed retrospectively to determine the frequency of surgical complications using the Fisher exact test and non-parametric testing. Results: Eighty-seven research subjects underwent a total of 95 LN excisions. Thirty-six percent of subjects were female, 53% were white, 26% were black, 16% Hispanic, and 2% Native American. Median age was 36 y (22-52). The median CD4+ T cell count was 478 cell/mm(3) (range, 57-1117) and the median plasma HIV-1 RNA concentration was 4.1 log10copies/mL (range, 1.7-5.9). Minor complications including seroma, transient lymphedema, hematoma, and allergic reaction to surgical tape, occurred in 10% of procedures. Complications that required medical attention occurred in an additional 10% of procedures, and included cellulitis (5%), superficial incisional surgical site infection (3%), and seroma requiring aspiration (1%). Subjects with complications had a lower BMI (25; range, 16-38; n=12) than others (28; range, 19-57; n=40; p=0.05) and tended to have higher platelets, (median, 259×10(9)/L; range, 196-332; vs. 233×10(9)/L; range, 44-633; p=0.07). No other clinical or laboratory characteristics were associated with complications (p≥0.3). Conclusions: Lymph node excision for research purposes is generally safe in a diverse group of chronically HIV-1-infected women and men, but can result in complications in a minority of subjects. No predictors of complications were identified.Surgical Infections 05/2014; 15(4). DOI:10.1089/sur.2013.218 · 1.45 Impact Factor
Article: CCR5 Expression is Reduced in Lymph Nodes of HIV-1-Infected Women Compared to Men, But Does Not Mediate Sex Differences in Viral Loads.[Show abstract] [Hide abstract]
ABSTRACT: Background. Human immunodeficiency virus type 1 (HIV-1)-infected women have lower viral loads than men but similar rates of disease progression. We hypothesized that sex-based differences in CCR5 expression mediate viral load differences.Methods. CCR5 was analyzed by flow cytometry in disaggregated lymph node cells from untreated HIV-1-infected women (n = 28) and men (n = 27). The frequencies of HIV-1 RNA-producing cells in the lymph node were determined by in situ hybridization. Linear and generalized linear regression models were used.Results. The percentage of CCR5+CD3+CD4+ cells was lower in women (mean, 12%) than men (mean, 16%; P =. 034). Neither the percentage of CCR5+CD3+CD4+ cells nor the CCR5 density predicted viral load or HIV-1 RNA-producing lymph node cells (P ≥. 24), after adjusting for CD4+ T-cell count, race, and age. Women had marginally fewer HIV-1 RNA-producing cells (mean, 0.21 cells/mm2) than men (mean, 0.44 cells/mm2; P =. 046). After adjusting for the frequency of HIV-1 RNA-producing cells and potential confounders, the viral load in women were 0.46 log10 copies/mL lower than that in men (P =. 018).Conclusions. Reduced lymph node CCR5 expression in women did not account for the viral load difference between sexes. CCR5 expression did not predict viral load or frequencies of HIV-1 RNA-producing cells, indicating that physiologic levels of CCR5 do not limit HIV-1 replication in lymph node. Less plasma virus was associated with each HIV-1 RNA-producing cell in women as compared to men, suggesting that women may either produce fewer virions per productively infected cell or more effectively clear extracellular virus. © 2013 The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: [email protected] /* */The Journal of Infectious Diseases 10/2013; 209(6). DOI:10.1093/infdis/jit575 · 6.00 Impact Factor
Article: Tumor necrosis factor-α inhibition improves endothelial function and decreases arterial stiffness in estrogen-deficient postmenopausal women[Show abstract] [Hide abstract]
ABSTRACT: Tumor necrosis factor (TNF)-α, a pleiotropic pro-inflammatory cytokine involved in a variety of biological processes including oxidative stress, has been associated with vascular dysfunction in aged and ovariectomized animals. We determined whether acute inhibition of TNF-α improves vascular endothelial function and decreases arterial stiffness in estrogen-deficient postmenopausal women. Arterial stiffness (carotid artery compliance) and endothelial function (brachial artery flow-mediated dilation [FMD]) were measured in postmenopausal women (n = 23; 57 ± 1 years, mean ± SE) before and following randomization to two days of either transdermal estradiol (0.05 mg/d, N = 12) or placebo (N = 11) alone and following a single subcutaneous injection of the TNF-α inhibitor, etanercept (25 mg), and in premenopausal (n = 9; 33 ± 2 years) before and following etanercept. Baseline carotid artery compliance and brachial artery FMD were lower in postmenopausal than premenopausal women (P < 0.0001). In postmenopausal women, carotid artery compliance (n = 12; 0.59 ± 0.05-0.78 ± 0.06 mm(2)/mmHg × 10(-1), P < 0.001) and FMD (4.1 ± 0.6-6.0 ± 0.7%, P = 0.02) increased in response to estradiol but not placebo (n = 11). Carotid artery compliance (0.71 ± 0.06-0.81 ± 0.06 mm(2)/mmHg × 10(-1), P = 0.02) and FMD (5.2 ± 0.7-7.5 ± 0.9%, P = 0.003) increased with etanercept in the placebo group but had no effect in postmenopausal randomized to estradiol or premenopausal women. These results suggest that TNF-α contributes to impaired endothelial-dependent vasodilation and arterial stiffening in estrogen-deficient postmenopausal women.Atherosclerosis 10/2013; 230(2):390-6. DOI:10.1016/j.atherosclerosis.2013.07.057 · 3.99 Impact Factor
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ABSTRACT: A reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of tenofovir (TFV) and emtricitabine (FTC) in dried blood spots (DBS) from human whole blood was developed and validated. Whole blood samples were spotted, dried, and a 3mm punch was extracted with methanol for analysis by LC-MS/MS utilizing stable isotope labeled internal standards. The assay was validated over the range of 2.5-1000ng/mL for TFV and 2.5-5000ng/mL for FTC. The method was accurate (within ±15% of control) and precise (coefficient of variation ≤15%) for hematocrit concentrations ranging from 25% to 76%; using edge punches vs. center punches; and spot volumes of 10-50μL. Analytes were stable for five freeze/thaw cycles and up to 6 days at room temperature, whereas long-term storage required -20°C or -80°C. Comparison of TFV and FTC in DBS vs. plasma yielded r(2)≥0.96, indicating that DBS can be used as a plasma alternative for pharmacokinetic analyses in vivo.Journal of pharmaceutical and biomedical analysis 08/2013; 88C:144-151. DOI:10.1016/j.jpba.2013.08.033 · 2.98 Impact Factor
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ABSTRACT: The majority of HIV-infected individuals fail to produce protective antibodies and have diminished responses to new immunizations. We report here that even though there is an expansion of follicular helper T (TFH) cells in HIV-infected individuals, the cells are unable to provide adequate B cell help. We found a higher frequency of programmed cell death ligand 1 (PD-L1)+ germinal center B cells from lymph nodes of HIV-infected individuals suggesting a potential role for PD-1-PD-L1 interaction in regulating TFH cell function. In fact, we show that engagement of PD-1 on TFH cells leads to a reduction in cell proliferation, activation, inducible T-cell co-stimulator (ICOS) expression and interleukin-21 (IL-21) cytokine secretion. Blocking PD-1 signaling enhances HIV-specific immunoglobulin production in vitro. We further show that at least part of this defect involves IL-21, as addition of this cytokine rescues antibody responses and plasma cell generation in vitro. Our results suggest that deregulation of TFH cell-mediated B cell help diminishes B cell responses during HIV infection and may be related to PD-1 triggering on TFH cells. These results demonstrate a role for TFH cell impairment in HIV pathogenesis and suggest that enhancing their function could have a major impact on the outcome and control of HIV infection, preventing future infections and improving immune responses to vaccinations.Nature medicine 03/2013; 19(4). DOI:10.1038/nm.3109 · 27.36 Impact Factor
Article: Endothelial Function Is Impaired across the Stages of the Menopause Transition in Healthy Women[Show abstract] [Hide abstract]
ABSTRACT: Context:The stages of the menopause transition are characterized by changes in ovarian hormones and increased cardiovascular disease (CVD) risk factors and vasomotor symptoms that may adversely affect vascular health.Objective:We tested the hypothesis that endothelial function, a predictor of CVD, would be reduced across the stages of the menopause transition, independent of CVD risk factors and vasomotor symptoms.Design, Setting, and Participants:This was a cross-sectional study of 132 healthy women from the general community aged 22-70 yr, categorized as premenopausal (n = 33, 32 ± 6 yr; mean ± sd), early perimenopausal (n = 20, 49 ± 3 yr) or late perimenopausal (n = 22, 50 ± 4 yr), or early (n = 30, 55 ± 3 yr) or late postmenopausal (n = 27, 61 ± 4 yr).Main Outcome:Endothelial-dependent vasodilation was measured by brachial artery flow-mediated dilation (FMD) using ultrasound.Results:Brachial artery FMD was significantly different among the groups (P < 0.001). It was highest in premenopausal women (9.9 ± 2.1%) with progressive decrements in perimenopausal (early: 8.2 ± 2.5%; late: 6.5 ± 1.9%) and postmenopausal women (early: 5.5 ± 1.9%; late: 4.7 ± 1.7%). Adjustment for risk factors, vasomotor symptoms, and sex hormones did not alter the association (P < 0.001). In subgroup analyses of women aged 50-59 yr, brachial artery FMD was lower in late peri- and early and late postmenopausal compared with early perimenopausal women (P < 0.001) but was not different between late perimenopausal and either early or late postmenopausal women.Conclusions:Our findings suggest that a decline in endothelial function begins during the early stages of menopause (perimenopause) and worsens with the loss of ovarian function and prolonged estrogen deficiency. These data add to the accumulating evidence that the perimenopausal window is a critical time period for adverse changes in CVD risk.The Journal of Clinical Endocrinology and Metabolism 09/2012; 97(12). DOI:10.1210/jc.2012-2244 · 6.21 Impact Factor
Article: Tenofovir, Emtricitabine, and Tenofovir Diphosphate in Dried Blood Spots for Determining Recent and Cumulative Drug Exposure[Show abstract] [Hide abstract]
ABSTRACT: Abstract Tenofovir (TFV) disoproxil fumarate (TDF)±emtricitabine (FTC) are widely used for HIV treatment and chemoprophylaxis, but variable adherence may lead to suboptimal responses. Methods that quantify adherence would allow for interventions to improve treatment and prevention outcomes. Our objective was to characterize the pharmacokinetics of TFV-diphosphate (TFV-DP) and FTC-triphosphate (FTC-TP) in red blood cells (RBCs) and peripheral blood mononuclear cells (PBMCs); to extend the RBC analysis to dried blood spots (DBSs); and to model how RBC/DBS monitoring could inform recent and cumulative drug exposure/adherence. Blood samples were collected from 17 HIV-negative adults at 5 visits over a 30-day pharmacokinetics study of daily oral TDF/FTC. Dosing was discontinued on day 30 and blood was collected on days 35, 45, and 60 during the washout period. Plasma/RBCs/PBMCs/DBSs were all quantified by liquid chromatography/tandem mass spectrometry. DBSs were paired with RBCs and plasma for comparisons. The median (interquartile range) RBC TFV-DP half-life was 17.1 (15.7-20.2) versus 4.2 (3.7-5.2) days in PBMCs. At steady state, TFV-DP was 130 fmol/10(6) RBCs versus 98 fmol/10(6) PBMCs. FTC-TP was not quantifiable in most RBC samples. TFV-DP in RBCs versus DBSs yielded an r(2)=0.83. TFV-DP in DBSs was stable at -20°C. Simulations of TFV-DP in RBCs/DBSs, when dosed from one to seven times per week, demonstrated that each dose per week resulted in an average change of approximately 19 fmol/10(6) RBCs and 230 fmol/punch. TFV and FTC in plasma versus DBSs was defined by y=1.4x; r(2)=0.96 and y=0.8x; r(2)=0.99, respectively. We conclude that DBSs offer a convenient measure of recent (TFV/FTC) and cumulative (TFV-DP in RBCs) drug exposure with potential application to adherence monitoring.AIDS research and human retroviruses 08/2012; 29(2). DOI:10.1089/AID.2012.0089 · 2.33 Impact Factor
Article: Effect of HIV-1 Infection and Sex on the Cellular Pharmacology of the Antiretroviral Drugs Zidovudine and Lamivudine[Show abstract] [Hide abstract]
ABSTRACT: The cellular pharmacology of zidovudine (ZDV) and lamivudine (3TC) in vivo is not completely understood. This prospective longitudinal study investigated the relationship between HIV-1 serostatus, sex, race, and time on therapy with intracellular and plasma ZDV and 3TC concentrations. Of 20 HIV-seronegative and 23 HIV-seropositive volunteers enrolled, 16 (8 women) and 21 (5 women) completed all 12 study days, respectively. Volunteers began ZDV-3TC therapy (plus a third active drug in HIV-seropositive volunteers), and steady-state concentrations (C(ss)) were determined after days 1, 3, 7, and 12. A repeated-measures mixed model was utilized. HIV-seronegative status was associated with 22% (95% confidence interval [CI], 0%, 50%) and 37% (15%, 67%) higher C(ss) estimates compared to those of HIV-seropositive individuals for intracellular ZDV-TP and 3TC-TP levels, respectively. African-Americans had 36% (8%, 72%) higher ZDV-TP estimates than non-African-Americans. Sex was not associated with ZDV-TP or 3TC-TP (P > 0.19). Women had 36% (4%, 78%) higher plasma ZDV, but the effect was lessened when normalized by lean body weight (5% [-19%, 38%]; P = 0.68). Plasma 3TC was 19% (0%, 41%) higher in HIV-seropositive volunteers and 22% (0%, 48%) higher in African American volunteers, but these effects were not significant when corrected for creatinine clearance (7% [-9%, 20%] and -5% [-26%, 12%] for HIV serostatus and race, respectively; P > 0.35). These results suggest that HIV-seropositive status decreases and African American race elevates the cellular triphosphates of ZDV and 3TC. This information extends knowledge of ZDV and 3TC cellular pharmacology in vivo and provides new leads for future cellular pharmacology studies aimed at optimizing HIV prevention/treatment with these agents.Antimicrobial Agents and Chemotherapy 03/2012; 56(6):3011-9. DOI:10.1128/AAC.06337-11 · 4.48 Impact Factor
Article: Tetrahydrobiopterin improves endothelial function and decreases arterial stiffness in estrogen-deficient postmenopausal women[Show abstract] [Hide abstract]
ABSTRACT: The mechanisms mediating arterial stiffening with aging and menopause are not completely understood. We determined whether administration of tetrahydrobiopterin (BH(4)), a critical cofactor for endothelial nitric oxide synthase to produce nitric oxide, would increase vascular endothelial-dependent vasodilatory tone and decrease arterial stiffness in estrogen-deficient postmenopausal women. Additionally, we examined whether the beneficial effects of estrogen on vascular function were possibly related to BH(4). Arterial stiffness (carotid artery compliance) and endothelial-dependent vasodilation [brachial artery flow-mediated dilation (FMD)] were measured in postmenopausal (n = 24; 57 ± 1 yr, mean ± SE) and eumenorrheic premenopausal (n = 9; 33 ± 2 yr) women before and 3 h after the oral administration of BH(4). Subsequently, in postmenopausal women, vascular testing (before and after BH(4)) was repeated following randomization to either 2 days of transdermal estradiol or placebo. Baseline carotid artery compliance and brachial artery FMD were lower in postmenopausal than in premenopausal women (P < 0.0001). BH(4) administration increased carotid artery compliance (0.61 ± 0.05 to 0.73 ± 0.04 mm(2)·mmHg(-1)·10(-1) vs. baseline, P < 0.0001) and brachial artery FMD (P < 0.001) in postmenopausal women but had no effect in premenopausal women (P = 0.62). Carotid artery compliance (0.59 ± 0.05 to 0.78 ± 0.06 mm(2)·mmHg(-1)·10(-1), P < 0.001) and FMD increased in postmenopausal women in response to estradiol (P = 0.02) but were not further improved with the coadministration of BH(4), possibly because estrogen increased BH(4) bioavailability. Carotid artery compliance and FMD increased with BH(4) in the placebo group (P = 0.02). Although speculative, these results suggest that reduced vascular BH(4) may be an important contributor to arterial stiffening in estrogen-deficient postmenopausal women, related in part to reduced endothelial-dependent vasodilatory tone.AJP Heart and Circulatory Physiology 03/2012; 302(5):H1211-8. DOI:10.1152/ajpheart.01065.2011 · 3.84 Impact Factor
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ABSTRACT: New HIV-1 infections are increasing in older American women largely through heterosexual transmission. Activated CD4+ T cells and CCR5 expression are linked to HIV-1 susceptibility, but whether these parameters are altered in the cervix of older women is unknown. Whole blood and in some instances endocervical brush samples were collected from healthy premenopausal (n = 22) and postmenopausal women (n = 24). Percentages of HLA-DR(DR)+CD38(38)+CD4+ T cells and HIV-1 chemokine coreceptor expression were determined by flow cytometry. Percentages of DR+38+CD4+ T cells were 6 times greater in cervix (median: 6.4%) than blood (median: 1.1%; P < 0.001) but did not differ within each compartment between premenopausal and postmenopausal women (P = 0.2). Postmenopausal women had greater percentages of CCR5+CD4+ and CCR5+DR+38+CD4+ T cells compared with premenopausal women in cervix (median: 70% vs. 42%, P = 0.005; and 80% vs. 57%; P = 0.05, respectively) and blood (medians: 22% vs. 13%, and 76% vs. 62%, respectively; P < 0.001). Postmenopausal women had more CCR5 molecules on cervical DR+38+CD4+ T cells (median: 3176) than premenopausal women (median: 1776; P = 0.02). Age and percent CCR5+CD4+ and CCR5+DR+38+CD4+ cells were linearly related in cervix (r(2) = 0.47, P < 0.001 and r(2) = 0.25, P = 0.01, respectively) and blood (r(2) = 0.20, P = 0.001 and r(2) = 0.31, P < 0.001; respectively), but confounding of age with menopause could not be excluded. Cervical CXCR4 expression did not differ substantially between premenopausal and postmenopausal women. Elevated cervical CCR5 expression in postmenopausal women may increase their risk for HIV-1 acquisition. Studies are needed to confirm whether elevated CCR5 expression confers increased HIV-1 susceptibility in postmenopausal women, and if it is related to hormonal or nonhormonal effects of aging.JAIDS Journal of Acquired Immune Deficiency Syndromes 11/2011; 59(3):221-8. DOI:10.1097/QAI.0b013e31823fd215 · 4.56 Impact Factor
Article: Virologic Failure with a Raltegravir-Containing Antiretroviral Regimen and Concomitant Calcium Administration[Show abstract] [Hide abstract]
ABSTRACT: Abstract Raltegravir, an inhibitor of the human immunodeficiency virus-1 (HIV-1) integrase enzyme, is a preferred component of antiretroviral regimens for both treatment-naïve and -experienced patients. Compared with other antiretroviral agents, raltegravir has less toxicity and fewer drug interactions since it is not a substrate, inhibitor, or inducer of cytochrome P450 enzymes. However, raltegravir is not devoid of drug interactions. We describe a 39-year-old, HIV-1-infected man who experienced virologic failure while receiving a raltegravir-containing antiretroviral regimen with concomitant calcium administration. The patient was prescribed protease inhibitor-based combination antiretroviral therapy, and within 2 months, he achieved undetectable plasma HIV-1 RNA levels. Seven months after starting therapy, he developed a focal cerebral vasculitis of unknown etiology, which was treated with prednisone, aspirin, and acyclovir. Due to the potential for drug interactions between prednisone and protease inhibitors, the patient was switched to an antiretroviral regimen containing raltegravir. In addition, calcium carbonate 1 g-vitamin D3 400 IU 3 times/day was started for prevention of osteoporosis associated with long-term glucocorticoid use. After 10 months of an undetectable plasma HIV-1 RNA level and clinical evidence of a high level of adherence, he subsequently developed detectable HIV-1 RNA levels with documented resistance to raltegravir. His antiretroviral therapy was changed back to a protease inhibitor-based regimen, and his HIV-1 RNA level rapidly resuppressed. Calcium binding to the divalent metal ion-chelating motif of raltegravir may have led to subtherapeutic raltegravir levels in this patient. It is important for clinicians to be aware of the potential interaction between polyvalent cation-containing agents, such as calcium, and raltegravir.Pharmacotherapy 10/2011; 31(10):1042. DOI:10.1592/phco.31.10.1042 · 2.66 Impact Factor
Article: HLA-DR+ CD38+ CD4+ T lymphocytes have elevated CCR5 expression and produce the majority of R5-tropic HIV-1 RNA in vivo[Show abstract] [Hide abstract]
ABSTRACT: Percentages of activated T cells correlate with HIV-1 disease progression, but the underlying mechanisms are not fully understood. We hypothesized that HLA-DR+ CD38+ (DR+ 38+) CD4+ T cells produce the majority of HIV-1 due to elevated expression of CCR5 and CXCR4. In phytohemagglutinin (PHA)-stimulated CD8-depleted peripheral blood mononuclear cells (PBMC) infected with HIV-1 green fluorescent protein (GFP) reporter viruses, DR− 38+ T cells constituted the majority of CCR5 (R5)-tropic (median, 62%) and CXCR4 (X4)-tropic HIV-1-producing cells (median, 61%), although cell surface CCR5 and CXCR4 were not elevated in this subset of cells. In lymph nodes from untreated individuals infected with R5-tropic HIV-1, percentages of CCR5+ cells were elevated in DR+ 38+ CD4+ T cells (median, 36.4%) compared to other CD4+ T-cell subsets (median values of 5.7% for DR− 38− cells, 19.4% for DR+ 38− cells, and 7.6% for DR− 38+ cells; n = 18; P < 0.001). In sorted CD8− lymph node T cells, median HIV-1 RNA copies/105 cells was higher for DR+ 38+ cells (1.8 × 106) than for DR− 38− (0.007 × 106), DR− 38+ (0.064 × 106), and DR+ 38− (0.18 × 106) subsets (n = 8; P < 0.001 for all). After adjusting for percentages of subsets, a median of 87% of viral RNA was harbored by DR+ 38+ cells. Percentages of CCR5+ CD4+ T cells and concentrations of CCR5 molecules among subsets predicted HIV-1 RNA levels among CD8− DR/38 subsets (P < 0.001 for both). Median HIV-1 DNA copies/105 cells was higher in DR+ 38+ cells (5,360) than in the DR− 38− (906), DR− 38+ (814), and DR+ 38− (1,984) subsets (n = 7; P ≤ 0.031). Thus, DR+ 38+ CD4+ T cells in lymph nodes have elevated CCR5 expression, are highly susceptible to infection with R5-tropic virus, and produce the majority of R5-tropic HIV-1. PBMC assays failed to recapitulate in vivo findings, suggesting limited utility. Strategies to reduce numbers of DR+ 38+ CD4+ T cells may substantially inhibit HIV-1 replication.Journal of Virology 08/2011; 85(19):10189-200. DOI:10.1128/JVI.02529-10 · 4.44 Impact Factor
Article: Sex, Race, and Geographic Region Influence Clinical Outcomes Following Primary HIV-1 Infection[Show abstract] [Hide abstract]
ABSTRACT: It is unknown whether sex and race influence clinical outcomes following primary human immunodeficiency virus type 1 (HIV-1) infection. Data were evaluated from an observational, multicenter, primarily North American cohort of HIV-1 seroconverters. Of 2277 seroconverters, 5.4% were women. At enrollment, women averaged .40 log₁₀ fewer copies/mL of HIV-1 RNA (P < .001) and 66 more CD4(+) T cells/μL (P = .006) than men, controlling for age and race. Antiretroviral therapy (ART) was less likely to be initiated at any time point by nonwhite women and men compared to white men (P < .005), and by individuals from the southern United States compared to others (P = .047). Sex and race did not affect responses to ART after 6 months (P > .73). Women were 2.17-fold more likely than men to experience >1 HIV/AIDS-related event (P < .001). Nonwhite women were most likely to experience an HIV/AIDS-related event compared to all others (P = .035), after adjusting for intravenous drug use and ART. Eight years after diagnosis, >1 HIV/AIDS-related event had occurred in 78% of nonwhites and 37% of whites from the southern United States, and 24% of whites and 17% of nonwhites from other regions (P < .001). Despite more favorable clinical parameters initially, female HIV-1-seroconverters had worse outcomes than did male seroconverters. Elevated morbidity was associated with being nonwhite and residing in the southern United States.The Journal of Infectious Diseases 02/2011; 203(4):442-51. DOI:10.1093/infdis/jiq085 · 6.00 Impact Factor
Article: Anderson PL, Kiser JJ, Gardner EM, Rower JE, Meditz A, Grant RM. Pharmacological considerations for tenofovir and emtricitabine to prevent HIV infection[Show abstract] [Hide abstract]
ABSTRACT: The use of antiretroviral medications in HIV-negative individuals as pre-exposure prophylaxis (PrEP) is a promising approach to prevent HIV infection. Tenofovir disoproxil fumarate (TDF) and emtricitabine exhibit desirable properties for PrEP including: favourable pharmacokinetics that support infrequent dosing; few major drug-drug or drug-food interactions; an excellent clinical safety record; and pre-clinical evidence for efficacy. Several large, randomized, controlled clinical trials are evaluating the safety and efficacy of TDF and emtricitabine for this new indication. A thorough understanding of variability in drug response will help determine future investigations in the field and/or implementation into clinical care. Because tenofovir and emtricitabine are nucleos(t)ide analogues, the HIV prevention and toxicity effects depend on the triphosphate analogue formed intracellularly. This review identifies important cellular pharmacology considerations for tenofovir and emtricitabine, which include drug penetration into relevant tissues and cell types, race/ethnicity/pharmacogenetics, gender, cellular activation state and appropriate episodic or alternative dosing strategies based on pharmacokinetic principles. The current state of knowledge in these areas is summarized and the future utility of intracellular pharmacokinetics/pharmacodynamics for the PrEP field is discussed.Journal of Antimicrobial Chemotherapy 11/2010; 66(2):240-50. DOI:10.1093/jac/dkq447 · 5.31 Impact Factor
Article: Effect of Antacids on the Pharmacokinetics of Raltegravir in Human Immunodeficiency Virus-Seronegative Volunteers[Show abstract] [Hide abstract]
ABSTRACT: Raltegravir's divalent metal ion chelating motif may predispose the drug to interactions with divalent cations. We determined whether a divalent cation-containing antacid interacted with raltegravir. Twelve HIV-1-seronegative subjects were enrolled in this randomized, prospective, crossover study of single-dose raltegravir (400 mg) with and without an antacid. Subjects underwent two intensive pharmacokinetic visits in the fasted state separated by a 5- to 12-day washout period. With simultaneous antacid administration, time to peak raltegravir concentration occurred 2 h sooner (P = 0.002) and there was a 67% lower raltegravir concentration at 12 h postdose (P < 0.0001) than with administration of raltegravir alone. The raltegravir area under the-concentration-time curve from 0 to 12 h and maximum concentration were unchanged with the addition of an antacid. Studies are needed to determine the clinical relevance of this interaction, whether it remains after multiple dosing to steady state, whether it is mitigated by temporal separation, and whether raltegravir interacts with divalent cation-containing vitamins, supplements, or foods.Antimicrobial Agents and Chemotherapy 10/2010; 54(12):4999-5003. DOI:10.1128/AAC.00636-10 · 4.48 Impact Factor
Article: Blood Myeloid Dendritic Cells from HIV-1-Infected Individuals Display a Proapoptotic Profile Characterized by Decreased Bcl-2 Levels and by Caspase-3+ Frequencies That Are Associated with Levels of Plasma Viremia and T Cell Activation in an Exploratory Study[Show abstract] [Hide abstract]
ABSTRACT: Reduced frequencies of myeloid and plasmacytoid dendritic cell (DC) subsets (mDCs and pDCs, respectively) have been observed in the peripheral blood of HIV-1-infected individuals throughout the course of disease. Accumulation of DCs in lymph nodes (LNs) may partly account for the decreased numbers observed in blood, but increased DC death may also be a contributing factor. We used multiparameter flow cytometry to evaluate pro- and antiapoptotic markers in blood mDCs and pDCs from untreated HIV-1-infected donors, from a subset of infected donors before and after receiving antiretroviral therapy (ART), and from uninfected control donors. Blood mDCs, but not pDCs, from untreated HIV-1-infected donors expressed lower levels of antiapoptotic Bcl-2 than DCs from uninfected donors. A subset of HIV-1-infected donors had elevated frequencies of proapoptotic caspase-3(+) blood mDCs, and positive correlations were observed between caspase-3(+) mDC frequencies and plasma viral load and CD8(+) T-cell activation levels. Caspase-3(+) mDC frequencies, but not mDC Bcl-2 expression, were reduced with viral suppression on ART. Apoptosis markers on DCs in blood and LN samples from a cohort of untreated, HIV-1-infected donors with chronic disease were also evaluated. LN mDCs displayed higher levels of Bcl-2 and lower caspase-3(+) frequencies than did matched blood mDCs. Conversely, LN pDCs expressed lower Bcl-2 levels than their blood counterparts. In summary, blood mDCs from untreated HIV-1-infected subjects displayed a proapoptotic profile that was partially reversed with viral suppression, suggesting that DC death may be a factor contributing to blood DC depletion in the setting of chronic, untreated HIV disease.Journal of Virology 10/2010; 85(1):397-409. DOI:10.1128/JVI.01118-10 · 4.44 Impact Factor
Top Journals
University of Colorado
Denver, Colorado, United States
- • Division of Infectious Diseases
- • Department of Medicine
- • Department of Surgery
United States Army Medical Research Institute for Infectious DiseasesФредерик, Maryland, United States


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In an early scene of HBO's new Aaron Sorkin drama The Newsroom, a college student asks the show's hero, Will McAvoy, why the U.S. is the best country in the world.
Summer Preview: Check out more must-watch new shows
It's a vapid question, to be sure, but Will's mean-spirited answer — he says simply that it isn't, and then rattles off a withering, Sorkinian litany of statistics about literacy, life expectancy and infant mortality that prove his point — is both electrifying and kind of depressing.
Will (Jeff Daniels, in his first TV series role) is a moderate Republican cable news anchor whose show, "News Night," has succeeded in the ratings because Will has played it safe, journalistically speaking. (One critic calls him the "Jay Leno of news anchors.") When we first meet him, he's in a rut, and his staff, none too impressed with his very public tantrum, has decided to seek other employment.
Borrowing from Network writer Paddy Chayevsky, who Sorkin mentioned in his acceptance speech when he won an Oscar for writing The Social Network, Will's mad as hell and he isn't going to take it anymore. But Sorkin says he's hoping to do more than spew venom.
See photos of the Newsroom cast
Exclusive video: A glimpse into HBO's Newsroom
VIDEO: Meet the News Night 2.0 team in The Newsroom trailer
He's also quick to note that The Newsroom isn't trying to lambast the press either. "I am absolutely not doing that," Sorkin says. "I think bad information, wrong information and no information are dangerous for America. ... [But] we're not doing the news; we're doing a show about people who do the news. Our set's on Stage 7 at Sunset Gower Studios — the same stage where they shot The Monkees. Our goals are no different than theirs."
The Newsroom premieres Sunday at 10/9c on HBO.


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E-Z UP® 15 ft. Mid-Zip Canopy SidewallItem # HN-EU026
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If I am the Minnesota Vikings, I am excited that I am facing a running back that has not been relevant for 3 years.
If in fact Brandon Jacobs does not play, it looks like Peyton Hillis will get the start. Hillis has not been good since he was with the Browns. He made it on the Madden cover and has been cursed since.
@Michael_Fabiano: Reports are now suggesting Peyton Hillis is likely to start tonight if Brandon Jacobs is out.Do you follow the NFL Draft? If so follow NFL Draft Diamonds on Twitter and Facebook for up to date NFL Draft News, NFL Draft Projections, and NFL Draft Rankings. Jesus is the only way to Salvation


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Tropers: Manis
Hello, my name is Allen. Im an aspiring author and have found this site very useful. Ive learned that the better I understand certain tropes in my writing, the better I can use them in the best and most creative ways. Tropes I've Begun:
- Lord of the Rings
- Star Wars I, IV, V, VI (I don't care much for II and III)
- Star Trek: First Contact
- The Princess Bride
- Willow
- Gladiator
- The Last Samurai
- Batman Begins / The Dark Knight
- Kyle XY
- The Universe
- Star Trek The Next Generation and Voyager
- Legend of the Seeker
- Crusoe
- Adventures Of Sherlock Holmes
- Heroes
- Mythbusters
- The History Channel... yes, the entire History Channel


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WPS Converter1.0
editor's review
WPS Converter is a straightforward application that facilitates the file conversion of Microsoft Works files (WPS format) to plain text documents or HTML items. Its range of features is quite limited, making the app accessible to all user levels, especially the ones with limited experience in conversion software.
The installation operation is rapid and uneventful, without requiring special user intervention. Once it finishes, you are greeted by a standard window with a WYSIWYG environment, where you can dive into the conversion procedure immediately and not worry about unnecessary graphical elements or complex steps.
WPF items can be loaded into the workspace using only the file browser, since the drag-and-drop functionality is not supported. Unfortunately, batch processing is not possible, so you can work with just one item at a time.
After you establish the output format, directory and file name, you may start the conversion procedure by clicking a button. The task is immediately performed, and WPS Converter doesn't display error dialogs, hang or crash in the meantime. As it would be expected from such a feature-limited tool, its impact on the system performance is minimal.
Evidently, some additional features would have been welcomed, such as the possibility of previewing files, processing WPS items in batch mode, or changing output file properties (e.g. font type and size). We must also take into account that the app has not been updated for a while. But, even so, WPS Converter provides the simplest method possible to fill TXT and HTML files with content from Microsoft Works documents.
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Canon PowerShot G1 X offers APS-C size sensor in a compact
Canon has pulled the covers off its latest high-end compact camera, the Canon PowerShot G1 X, pitched at professionals and enthusiasts. It steps in where the PowerShot G12 left off, but now offers a CMOS sensor that measures 18.7 x 14mm - close to the APS-C size in their EOS cameras.
The new sensor is 4:3 aspect and offers you 14.3-megapixels. As a CMOS sensor it offers the benefits of on-chip noise reduction, claiming clear images at high ISOs. The PowerShot G1 X offers a standard shooting range of ISO 100-12,800.
The new sensor is backed by Canon's Digic 5 processor, recently seen in the Canon SX40 IS and included in the new Ixus models launched in parallel to the G1 X.
The larger sensor will appeal to those looking to get extra quality out of their compact camera, but this is an undoubtedly large beast, weighing 534g and measuring 116.7 x 80.5 x 64.7mm.
The 4x lens offers you a 28-112mm range (in 35mm terms) and you get 4-stops of optical image stabilisation, with a max aperture of F/2.8. The lens includes a 3-stop ND filter, which will allow you greater creative freedom to use a range of settings without overexposing.
Full manual controls are offered and at first glance the controls look similar to the G12 and reflect those from Canon's DSLR range. Around the back you get a 3-inch vari-angle display, with a 920k-dot resolution. You'll also get a pop-up flash and an optical viewfinder to aid composition, along with a hotshoe for adding accessories. As before, you'll be able to attach 58mm filters via an adapter and get a waterproof case.
In terms of video capture you now get 1080p support at 24fps and the HDMI means you'll be able to easily share those movies on the big screen.
The Canon PowerShot G1 X will be available in late February and launches at £699, which makes it more expensive than the Canon EOS 600D, quite a price for a compact camera.
Canon have been good enough to put together a promo video for the camera, below, for your viewing pleasure.
Have Canon hit the mark with the PowerShot G1 X? Let us know in the comments below...
Logi Circle: Smart filtering video camera could get addictive
Best GoPro: Which GoPro should you choose?
GoPro unveils affordable Hero+ action cam with Wi-Fi, Full HD 60fps capture
The best GoPro photos in the world, prepare to lose your breath
- Best GoPro: Which GoPro should you choose?
- GoPro unveils affordable Hero+ action cam with Wi-Fi, Full HD 60fps capture
- The best GoPro photos in the world, prepare to lose your breath
- Win one of 10 PhotoBox Photo Books and five Retro Print sets
- X-Steady Electro handheld rig for action-cams and phones keeps vids silky smooth
- Sony a7S II can record 4K video in ridiculously low levels of light, ISO up to 409,600
- Pocket-lint Extreme: Thrashing a Jaguar XF around the track
- VR filmmakers can register for a GoPro Odyssey 360-degree shooting rig now, works with Jump
- Forget 4K, Canon's new 250MP sensor can read lettering on a plane 18km away
- 7 ways to make more of your Instagram photos
- When is Android 6.0 Marshmallow coming to my phone?
- Which iPhone is best for you? iPhone 5S, iPhone 6, iPhone 6S, iPhone 6 Plus or iPhone 6S Plus?
- HP might debut a Star Wars-themed Windows 10 device this week
- OnePlus Mini: What's the story so far?
- Siri, turn off my lights: Philips Hue with Hue Bridge 2.0 tested
- Forza Motorsport 6 review: The Forza is strong with this one
- Microsoft October 2015 launch event: What devices to expect
- Hanging around with Nathan Drake: Pocket-lint chats to Nolan North about gaming superstardom
- Can't afford the Xbox One Elite controller? Don't worry, you'll be able to remap your existing controller buttons
- Samsung Galaxy View is an iPad Pro rival with a crazy 18.5-inch screen, amazing leaked specs reveal all


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August 28, 2012
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Soirée: Potbelly Sandwich Shop Coming to Little Rock
Owner Ryan Hamra confirms Potbelly Sandwich Shop is coming to midtown in Little Rock this fall
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Whispers Blogby Arkansas Business Staff
- Governor: Arkansas Will Be in 'Top Tier' of Trading Partners with Cuba
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- Angelica Rogers Hit With $15.6M Consent Judgment 1 day ago
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Now playingMonday, 10/12/15
Watch the full episode of Monday night's The Rachel Maddow Show.
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Wednesday's Mini-Report, 9.9.15
Some Iran deal critics admit they're ready for war
In a speech slamming President Obama's Iran deal -- which Congress is debating this week -- former Vice President Dick Cheney suggested that only the threat of military action could prevent Iran from developing a nuclear weapons program."As soon as President Obama went on Israeli TV and effectively ruled out the option of force, the Iranians knew that they had won," Cheney said, speaking Tuesday at the American Enterprise Institute.
"While lashing out against critics, most of the essays avoid any mention of waterboarding, rectal feeding..." https://t.co/NN7NlzEMk8— Rachel Maddow MSNBC (@maddow) September 9, 2015
Jeb to repeat his brother's mistakes, expects different results
...I want to lower taxes and make the tax code simple, fair and clear. It should be easy to understand and make it easy for people to fill out their own tax forms.We will cut individual rates from seven brackets to three: 28%, 25% and 10%. At 28%, the highest tax bracket would return to where it was when President Ronald Reagan signed into law his monumental and successful 1986 tax reform.
Wednesday's Campaign Round-Up, 9.9.15
The rise of the other inexperienced, unqualified candidate
In a primary where Trump's bombast and braggadocio have given him indefatigable popularity, Carson's contrasting humility and soft-spoken demeanor -- paired with his outsider credibility and far-right views -- has wowed voters across the country."Ben Carson may be the perfect answer to people who are sick and tired of traditional politics and the politicians that practice it, but without the pomp and arrogance of Donald Trump," Republican strategist Rich Gallen told msnbc. "He's got Jeb's thoughtfulness and Trump's outsiderness."
Congressional GOP can't even cook the books properly
The new Republican-appointed director of the Congressional Budget Office delivered some bad news ... to the party's "Reaganomics" devotees: Tax cuts don't pay for themselves through turbocharged economic growth.Keith Hall, who served as an economic adviser to former President George W. Bush, made the pronouncement at his first news conference after the CBO reduced its 2015 budget deficit forecast by $60 billion.
Kim Davis puts marriage fight on GOP front-burner
Just when they thought they were out, Kim Davis pulled them back in.Republican strategists are worried that the return of same-sex marriage as a presidential campaign piñata could hurt the party in the 2016 general election, putting it on the wrong side of a growing majority of Americans that believes gay couples should have the right to marry. National Republicans operatives hoped the issue was settled in June when the Supreme Court ruled to legalize same-sex marriage nationwide.But Davis upended that.
Looking for options, Graham targets IAEA
Speaking to a few dozen people at a "No Nukes for Iran" town hall meeting in his home state, Graham also said Monday that he plans to block the transfer of $88 million in U.S. funds to the International Atomic Energy Agency until Congress gets access to so-called "side agreements" related to the Iranian nuclear agreement.Graham has power in this situation because of his role as chairman of the Appropriations Subcommittee on State and Foreign Operations.... Graham said that without the military facilities being subject to inspection, it would be impossible to determine the prospect of development.
Walker's new posture: 'There is no such thing as a hypothetical'
Staying constant, however, has been one of his biggest challenges. On key issues of the day -- from calls to end birthright citizenship to the jailing of a Kentucky county official who refused to issue same-sex marriage licenses -- Walker has struggled more than other candidates to clearly explain where he stands.On several issues, he has attempted to not take a side -- including when asked this week what he would do to directly address the crisis facing Europe as hundreds of thousands of refugees from Syria and other war-torn countries search for a safe place to live.
Christie picks the wrong day to complain about 'lawlessness'
The chairman and CEO of United Airlines is stepping down in connection with an investigation into the airline's dealings with the agency that operates New York airports.United Continental Holdings Inc. said Tuesday that Jeff Smisek and its executive vice president of communications and government affairs and its senior vice president of corporate and government affairs had resigned.
EU plan to take 160,000 refugees and other headlines
European Union calls for mandatory refugee quotas. (USA Today)
Australia to take 12,000 more. (New York Times)
TV camerawoman fired after tripping migrants fleeing Hungary cops. (NBC News)
Ben Carson calls for guest-worker status for immigrants. (AP)
Clinton to weigh in on Iran deal as Trump and Cruz try to crush it. (New York Times)
Hillary Clinton address concerns over email server... in an email. (Huffington Post)
State department to shift 50 staff to boost office handling Clinton emails. (Reuters)
Russia confirms it has military advisers in Syria. (New York Times)
Marine Corps' women-in-combat experiment gets mixed results. (Washington Post)
Citations for the September 8, 2015 TRMS
Tonight's guests:
- Patrick Murray, director of the Monmouth University Polling Institute
- Senator Dick Durbin
Tonight's links:
About The Rachel Maddow Show
Launched in 2008, "The Rachel Maddow Show" follows the machinations of policy making in America, from local political activism to international diplomacy. Rachel Maddow looks past the distractions of political theater and stunts and focuses on the legislative proposals and policies that shape American life - as well as the people making and influencing those policies and their ultimate outcome, intended or otherwise.


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Let Us Find The Reason Why Bertrand Grospellier Is Coming In Las Vegas For 46th WSOP
27th May 2015 is going to be the luckiest day for poker lovers as well as for poker stars to show their calibre in the 46th WSOP in Las Vegas. Fans have given nickname to Bertrand as Elky and they will be able to see his magical dealing with playing cards. The calibre of this poker star is to mix the playing cards with magical way. He is master in mixing the cards and able to display the cards as a cord. This art and its secret are only with the Elky. The scheduled match and opponent both are quite famous and having the calibre to grab the title of 46th WSOP.
Presently Elky is residing in London but his daily routine is to participate in any poker tournament of International level across the world. He is also known as "Triple Crown" because he has bagged three reputed trophies of poker in recent past. Huge prize money has been gambled on his name. This is also a challenge. This 34 years old poker star has calibre to lift the title and able to give a clean sweep to the opponents. His famous trick is the mixing. He mixes the playing cards in very quick manner and opponents cannot judge that on which number trump card is hidden. This magic is only known to none other than Elky. Read more »


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Printable Area
Photo by Zurie
Photo by Zurie
"This recipe is from Claire Robinson on the Food Network and looked absolutely TO DIE FOR! It's really similar to one we have made in the past at our family gatherings. I am trying this for Thanksgiving this year! I might add a little white pepper, bacon or some red pepper flakes...but I have to try the original version first!"
Serving Size: 1 (88 g)
Servings Per Recipe: 6


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Who We Are
CLO and Co-founder, M.Ed. — Tracy is a learning strategist, instructional designer and entrepreneur. She designs e-learning programs learners actually enjoy taking and focuses on creating meaningful change in the workplace. She is responsible for Weejee's unique brand of fun learning. More »
CEO and Co-founder — Ian works on strategic issues day-to-day at Weejee. His focus is on company-wide strategy, product and business innovation, and key initiatives. Ian's areas of specialty include social learning, learning technologies, digital and social strategy, and filtering fun into things. More »
VP of Client Care
Sun. Lake. Boat. Waterski. Woohoo! I also love singing in the car, taking pictures of my husband and two girls. And organizing closets!
VP of Sales
I have more interests than time. Hockey, fishing, exercising, spending time in the woods with my Vizsla. But a good family night with my wife and kids is tough to beat.
Director of Technology Services
I love being at the lake with family and friends. I enjoy finding great farm-to-table restaurants that use local ingredients. Oh, and of course cheering on my Boston sports teams!
Talent Acquisition Director
Fashion, great food and wine, travel are my passions. I can also be caught watching BBC shows on Netflix at home with my sweet son, my amazing hubby, and our mellow chocolate lab.
Senior Instructional Designer
I love warm weather and spend as much time as possible at the beach or in the pool. I also enjoy going for walks and watching football. Go Pats!
Creative Project Manager
I am an eternal dreamer and avid adventurer. I don't just try to think outside the box, I try to live there. I love traveling with my family and can't wait to show my kids the world!
Creative Project Manager
I'm a yoga teacher, and I love trail walks with my dog, Ollie. I love learning! Specifically foreign languages, which makes traveling to new places fun and exciting.
Senior Instructional Designer
My love language is homemade food. I'm a runner (to keep up with my toddler), and my free time is filled with family, the outdoors, and supporting new moms as a volunteer LC.
Graphic Designer
I've been an artist since the age of 5, when I discovered Crayola crayons and bedroom walls. Aside from making things look awesome, I like motorcycling, playing the guitar, and hockey. Go Flyers!
Quality Assurance
I'm an avid historical fiction reader, I like to speed-walk and body surf, and I'm addicted to crossword puzzles and Sudoku. Love time with my husband, two daughters and our crazy dog, Max.
Executive Assistant
I love new experiences – exploring new cities, eating at new restaurants, and trying new recipes. I also love spending time at home reading a good book, knitting, and spending time with my family.
Instructional Designer
I get covered in dirt from my gardens, tinker with antique toy restoration and am known for my comedic "eccentric character" roles in community theatre. Love friends, family, and frivolity!
Push the boundaries of learning.
People work at Weejee because our approach is fresh, we're serious yet easy-going, and because it's exciting.
Join the fun!


text:
A&E (ended 2012)
well i really like it so....
No, only if they have their wife and kiddies and their dog in the show too which is what Family Jewels has. BTW, your suggestion about not having anymore shows about rock stars is pretty good. I'll agree with it. Too bad I'm not a TV programmer.
I agree with that! Nick is one of the best things about the show! But over all this is a great show! It is really funny and very entertaining (sp) to watch! And another thing is that they are way better than the ozbornes (sp)! I think most rock star families are anyways!
But seriously, they are a great family and it is really sweet how everyone gets along! Even my mom likes it!
I think it's better than the Osbournes in some ways. I'd rather watch Shannon over Sharon Osborune any day. Everytime I watched Ozzy I couldn't understand what the hell he was saying, at least Gene comes across as a bit more understandable. The kids are better looking and seem to have a decent head on their shoulders. It ain't that bad of a show.
the concept of the show is a copy.
real? i don't know but i think they know they are being filmed so there might be some sort of act going own.
I believe that A&E ordered 10 episodes. Tv.com only lists 7 at the moment, because the titles and airdates for the rest are not available yet.
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Founded in 2000, Big Moves is the only service organization in the world dedicated to getting people of all sizes into the dance studio and up on stage. Home to emFATic DANCE, our shows present a vision of a world transformed, where exciting performances can and do come from artists who resist the narrow dance-world norms….and narrow dancewear options. Learn more about us!
To see so many people supporting each other and dancing together regardless of ability, body type, or background fills me with joy and hope.–Minda Quickel, emFATic DANCE member.
Photos by Lisa J. Ellis.
Upcoming Events
Next Open Rehearsal 9/19
As you can see in our event listings we have a really busy number of...
No Open Rehearsal 7/4!
After a really busy production season the emFATs are taking a week off, so we...
Fat Reign THIS WEEKEND (6/13-6/14) in Oakland!
We are so excited we can barely stand it! Fat Reign is THIS Saturday (6/13) and Sunday (6/14)...
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Georgia Mega Millions Winner
Updated: 09/18/2006 - A Bainbridge man has won $163 million from the 'Mega Millions' jackpot.
Updated: 09/18/2006 - A Bainbridge man has won $163 million from the 'Mega Millions' jackpot.
Updated: 09/18/2006 - The Florida Coastal Ocean Observing System Caucus is a group of marine science interest groups meeting in Tallahassee, looking for ways to beef up marine research.
Updated: 09/18/2006 - An abduction alert has been issued for a girl possibly taken to Florida.
Posted: 09/18/2006 - A shooting at a local apartment complex landed one man in the hospital.
Posted: 09/18/2006 - The door of the historical St. Marks Lighthouse opened for the first time in years.
Updated: 09/18/2006 - A lucky winner of Georgia's Mega Millions Jackpot has struck gold. The winning ticket was purchased at a convenience store in Bainbridge, Georgia.
Updated: 09/18/2006 - A man is in critical condition after being run over by train.
Updated: 09/18/2006 - It was music to the ear at the 11th Anniversary Celebration of Stubbs Educational Foundation, taking place at Tallahassee Community College.
Updated: 09/18/2006 - The Energy Information Administration says prices at the pump aren't so painful this month. Just a month ago consumers were spending around $3.00 a gallon.
Updated: 09/18/2006 - The U.S. Department of Justice says one-third of American women report being beaten by their spouse. For one Taylor County family, this ongoing problem has become a reality where a loved one has sustained life threatening injuries.
Updated: 09/18/2006 - A touch of the Bahamas hit North Florida Fairgrounds. Phi Beta Sigma of Florida A&M University sponsored the Fourth Annual Goombay Festival, raising money for the March of Dimes.
Updated: 09/18/2006 - The Boys and Girls Club was kicking off a day of celebration across the entire nation as over 2,000 cities took part in America's Day for Kids.
Updated: 09/18/2006 - In another crime near South Adams Street, Florida A& M police are searching for an armed robber and need your help.
Updated: 09/18/2006 - Leon County participated in a vehicle and equipment auction. Twice a year Tallahassee residents come out to North Florida Fairgrounds, making some great buys.
Updated: 09/18/2006 - A group of illegal aliens arrested in Tallahassee face a judge in federal court.
Updated: 09/18/2006 - Popular amendments to the Florida state constitution like the workplace smoking ban and free pre-kindergarten are the result of citizen led ballot initiatives, but a new proposed amendment would make it harder for citizens' initiatives to pass.
Updated: 09/18/2006 - For the third time in nearly a year a federal judge has stopped the state of Georgia from enforcing a voter identification law.
Updated: 09/18/2006 - Democrats Jim Davis and Daryl Jones were in Tallahassee with a message of change. The two were talking of rising property taxes and escalating insurance costs after they promised to find solutions to both.
Updated: 09/18/2006 - A fight Thursday in Tallahassee's Tom Brown Park resulted in heightened security Friday at a nearby high school.
Updated: 09/18/2006 - A recent arson at a Tallahassee apartment complex is still under investigation, but now it's taking an unusual turn.
Updated: 09/18/2006 - They've tried time and time again to steal ATM machines in Tallahassee, but deputies and police can't seem to pin them down.
Updated: 09/18/2006 - The loss of some prominent people in the Quincy Police Department has city officials looking for a few good men and women.
Updated: 09/18/2006 - A study shows approximately 25 percent of Florida's population have some type of disability.
Updated: 09/18/2006 - Students at Tallahassee Community College are learning how to stay safe on campus. Students gathered for the first ever "Safe in the City."
Updated: 09/18/2006 - The student dropout rate is on the rise in Florida. Now the Leon County School District has a brand new public charter high school in Tallahassee, allowing students to step back into the classroom.


text:
Gingerr was relaxing and having some ice cream when Preston walked up to press his luck. He flashed a wad of cash after he broke the ice with her and she decided to let him tag along while she shopped for a bit. The flirting continued until Preston suggested they find somewhere else to get to know each other a little more. Gingerr could not agree more and she went off and hopped into his car with him. They got frisky and Ginferr licked and sucked his cock for quite some time. Preston got the genius idea to go to this house that had been abandoned for months in that area. Gingerr really let her inner vixen out once they were somewhere more secluded. Preston even offered this knockout redhead an apartment completely paid for so that he could pass by and get him some Gingerr any time he felt the need. Something tells me he will be over her new apartment very often.
"Rubbed Right"
Natasha Vega
"Aim To Please"
Ginger Maxx
"Corner Loving"
Alina West
"Here Pussy Pussy"
Skyler Haze
"Something To Suck On"
Rachel James
"Lucy Blew"
Lucy Tyler
"Nasty Noir"
Belle Noire
"Poison Ivy"
Ivy Vider
"Made To Order"
"Suck It Softly"
Michell Devine


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Latest Headlines
Latest Headlines
Pharma ad budgets to dip in 2016, but J&J, Pfizer, Roche and Novartis should still top $2B
Flat news is good news. Pharma spending on advertising and promotional expenses will dip by just 1.7% next year, according to Schonfeld & Associates' annual Advertising Ratios & Budgets. That means a total of more than $21 billion in ad spending and just a bit healthier than last year's decline of 2%.
Deal buzz: Forget Pfizer. Traders bet GSK as a match for J&J, Roche
Another week, another rumor about the target on GlaxoSmithKline's back. This time, U.K. traders were talking up a different potential takeover--not by Pfizer, but by Johnson & Johnson or Roche.
Pfizer supplements its China production with $90M plant
Pfizer has broken ground on a $90 million facility that is being built in Suzhou, China, it announced Monday. The additional capacity is needed as demand for its supplements grows in the Asia-Pacific region, particularly in China.
Takeda taps Sanford for robotics, 3-D modeling skills in heart failure pact
Sanford-Burnham Medical Research Institute is about to apply its robotics and 3-D modeling skills to the discovery of molecules to treat heart failure. Takeda is bankrolling the two-year program as part of its broader collaboration The Sanford Consortium for Regenerative Medicine.
Pfizer's Pristiq antidepressant comes up short for kids
When the FDA approved Wyeth's antidepressant Pristiq in 2008, the nod came with a number of provisions to do postmarketing studies, including one in pediatric patients. Seven years later, Pfizer, which bought Wyeth in a 2009 megamerger, has fulfilled that obligation to disappointing results. The drug failed to hit its endpoint.
Pfizer in quick succession turns aside second lawsuit alleging Zoloft birth defects ties
Just weeks after winning the first case to go to trial that accused Pfizer of hiding birth defect risks for the children of women who took the antidepressant Zoloft during pregnancy, the pharma giant has again convinced a jury that there was no credible connection. The results are positive for Pfizer, but the company still faces more than 1,000 lawsuits alleging similar claims.
Inflectra vs. Remicade study could be just what the doctor ordered for biosimilars
A study released today that pitted Hospira's biosimilar Inflectra against its reference drug Remicade in patients with rheumatoid arthritis is good news for Hospira, and by default Pfizer. It found that patients who were switched to the copy did just as well, with no unexpected side effects, as they had on Merck and Johnson & Johnson's Remicade.
Mylan expands cancer drug recall on particle contamination worries
Sterile injectable drugs are notoriously tricky to manufacture. Mylan has found that out the hard way, and it's now expanding a recall of cancer drugs that it began in April because they may contain particulate.
Pfizer scientist warned execs about Zoloft and birth defects, lawyers say
Pfizer executives were warned by one of their own scientists about a potential link between the antidepressant med Zoloft and birth defects, accordingly to a newly surfaced document cited by Bloomberg.
Mylan expands recall of cancer meds made for Pfizer
Sterile injectable drugs are a growth area, but they are notoriously tricky to manufacture. Mylan has found that out the hard way and is having to expand a recall of cancer drugs that it began in April because they may contain particulate. Some of the drugs were manufactured for Pfizer.


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Crosby looks to continue torrid pace at Consol
Penguins/NHL Videos
Consol Energy Center isn't really new anymore, as the Penguins will begin their third season there tonight against the Toronto Maple Leafs.
Seeing a healthy Sidney Crosby skate in the building is still pretty fresh, however.
Crosby has only played in 32 career games at the Penguins' home, and for him, tonight's opener will be special.
"Every game is big," he said. "But we want to make sure we get off to a good start at home. That's important."
He has made the most of those 32 appearances at Consol Energy Center.
Crosby has produced 23 goals and 56 points in those 32 games, good for a point per game average of 1.75. To put that number in perspective, only Wayne Gretzky and Mario Lemieux finished their careers with a point per game average of higher than the 1.75 that Crosby produces at Consol Energy Center.
Crosby averaged 1.52 points per game at Mellon Arena and produces 1.24 points per game on the road in his career. His lifetime point per game average of 1.39 is fourth all time, behind only Gretzky, Lemieux and Mike Bossy.
What is it about Consol Energy Center that brings out the best in Crosby?
"I actually think the fans have a lot to do with it," said right wing Pascal Dupuis, who has skated with Crosby during most of his appearances at the Penguins' third-year home.
"I don't know, there's just something about this place. The atmosphere is special. The crowd gets going, it's a beautiful place. We all love playing here."
Crosby has been exceptionally consistent at the team's third-year home. He has earned at least one point in 27 of the 32 games he has played at Consol Energy Center.
In those 32 games, Crosby has produced three four-point games, five three-point games and eight two-point games.
The Maple Leafs do not boast a particularly strong history of shutting down Crosby in any venue. He has schooled the Maple Leafs for 17 goals and 35 points in only 22 games.
They are well aware of the presence he brings.
"I know people aren't paying to watch me play hockey," Toronto defenseman Mike Komisarek said. "But I do know that they're paying to watch Sidney Crosby play tonight, just like they always do. We know he's going to be flying out there tonight. Tough test for us."
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Seattle holds off Baltimore for 22-17 victoryBy TIM BOOTH, AP Sports Writer Monday, Nov 14, 2011
SEATTLE (AP)—Even during Seattle's most crucial drive, Marshawn Lynch(notes) was busy joking with his teammates in the huddle about making sure his Skittles were waiting for him on the sideline.
Lynch earned his treat after the thumping he took and handed out Sunday against Baltimore.
"The man walks aggressively. Everything about him is moving forward aggressively and trying to gain yards. You've got to love that," Seattle fullback Michael Robinson(notes) said.
Lynch finished with 109 yards rushing and Seattle's lone touchdown, Steven Hauschka(notes) matched a franchise record with five field goals, and the Seahawks forced three turnovers in a 22-17 win over the Ravens.
Seattle snapped a three-game losing streak and picked up a second surprising win over a division leader after beating the New York Giants last month. And they added yet another flop to Baltimore's resume that already included slip-ups earlier this season at Tennessee and at Jacksonville, all three losses coming after important wins for the Ravens (6-3).
Lynch was at his best in the closing minutes when Seattle finished off the game in control of the ball, a perfect punctuation to a game the Seahawks mostly controlled. Seattle ate up the final 5:52 of the game, never giving Baltimore one last chance after Joe Flacco's(notes) 11-yard touchdown pass to Ed Dickson(notes) pulled the Ravens within five.
The Seahawks final drive ate up the final 5:52, advancing 62 yards and picking up four first downs before Tarvaris Jackson(notes) closed out the win by taking a knee. On the last possession, Lynch touched the ball on eight of the 12 plays. Seven of those were runs for 32 yards and he caught a crucial 8-yard pass on third-and-5 at the Baltimore 46 with 3:10 remaining.
But it wasn't a simple catch. Lynch added to his career highlight reel. He caught the pass three yards short of the first down, faked out Ravens linebackers Ray Lewis(notes) and Jarret Johnson(notes) so bad they were left grasping at air, and scampered ahead for the needed yardage.
"All he was talking about the middle of that last drive was somebody give him some Skittles," Robinson said. "That's the type of guy he is. He's a fun guy and I love blocking for him."
Hauschka kicked field goals of 22, 38, 39, 35 and 30 yards. Jackson was 17 of 27 for 217 yards and Lynch had another five receptions and 58 yards receiving. He became the first Seattle running back to record consecutive 100-yard rushing games since Julius Jones(notes) early in the 2008 season, following up on his 135 yards rushing last week against Dallas. Lynch carried a career-best 32 times, and most of those equaled short gains, but the types of yards Seattle needed against Baltimore's defense.
That proved to be enough thanks to Seattle's stout defense and special teams mistakes by the Ravens. David Reed(notes) fumbled twice on kickoff returns, leading to six points for Seattle. Flacco's lone interception was returned by David Hawthorne(notes) inside the Ravens 10 and eventually led to another field goal from Hauschka. Along with Reed's two fumbles, Billy Cundiff(notes) missed field-goal attempts of 52 and 50 yards in the first half.
Flacco was 29 of 52 for 255 yards, the 52 attempts for Flacco setting a new career-high. Ray Rice(notes), who was visibly upset after the Ravens had to settle for a 35-yard field goal late in the third quarter, had five carries for 27 yards and the Ravens had eight total carries by their running backs. Rice did catch eight passes for 54 yards, but receivers Torrey Smith(notes) and Anquan Boldin(notes) had five combined catches.
Carroll said he hoped Baltimore would abandon the run and try to throw its way past the Seahawks.
"I was kind of hoping that it would go like this, where they wouldn't feature (the run) as much so they wouldn't be balanced out," Carroll said. "We got up enough at halftime they decided they were going to throw."
The loss left the Ravens trying to explain a third flop this season against a losing team, this one coming a week after staking claim to the lead of the AFC North with a thrilling last-minute win at Pittsburgh.
After routing Pittsburgh in their opener, the Ravens were dominated in a loss at Tennessee. Just a few weeks ago, after an impressive win over AFC South-leading Houston, the Ravens were beaten by Jacksonville.
These moments have been rare since John Harbaugh took over in 2008, but three of them have popped up this season.
"There's the hard way and there is the easy way and there is the way we do it. It's like, let's see how much we can spot them before we come back and win the game," Baltimore linebacker Terrell Suggs(notes) said. "We can't do that in this league."
Dickson had a career-high 10 catches for 79 yards and two touchdowns for the Ravens, including a 1-yard TD pass from Rice in the second quarter. But Seattle's secondary locked up the Ravens' receivers and played as well as Baltimore's more lauded defense.
""It felt great. Especially when you've got big names out there. I'm just excited," Seattle safety Earl Thomas(notes) said. "I feel like our defense played well. We were the best defense out there today and it showed and we got the W."
NOTES: Seattle lost G John Moffitt(notes) (knee), WRs Sidney Rice(notes) (concussion) and Doug Baldwin(notes) (concussion), DB Atari Bigby(notes) (hamstring), DE Anthony Hargrove(notes) (hamstring) and SS Kam Chancellor(notes) (concussion) during the game. None returned. … Baltimore gave up just one sack of Flacco despite 52 pass attempts. … Seattle and Baltimore are tied 2-2 all-time.
Follow Tim Booth on Twitter: https://twitter.com/ByTimBooth
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- How the West was won: Rangers clinch division, Astros claim wild card
- How bad are Bucs? Panthers DB says he planned celebration before pick-6 of Jameis Winston
- The MLB postseason field is set! Here's the schedule for the opening rounds
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text:
Dansand® is Denmark's leading specialist in sand. We manufacture 350,000 tonnes of sand per year, in more than 20 different gradings - as well as a wide range of special products. Our fine quartz sand or silica sand is excavated from the subsoil in central Jutland and is the product of a 22 million year old geological process.
For us at Dansand® sand is not just sand. We are very focussed on our customers' needs and give high priority to product development. We are also market leaders when it comes to eco-friendly alternatives. Our experience, expertise and enthusiasm have made us the preferred partner - both in the professional industry and on DIY markets.
Dansand® exports approximately 1/3 of the production to neighbouring markets such as Germany, Norway, Sweden, England, France and Poland, but also to Saudi Arabia, Kuwait, USA and the Caribbean.
Regnvandsbassinet ved motorvejsstrækningen mellem Aarhus og Silkeborg har fået lidt opmærksomhed i fagmagasinet Grønt Miljø. Der stilles i artiklen blandt andet skarpt på de nye teknologier, som på sigt kan gøre håndteringen af regnvand langt mere effektiv.
"Den anvendte teknologi har aldrig tidlig
Lervejdal 8b, AdditDK-8740 Brædstrup
Tel. +45 86 82 58 11Fax +45 86 80 14 72


text:
Visit Website
Located on 8 storybook, private acres,with a large pond, waterfall, heated pool, two fireplaces and outdoor hot tub. The 4 rooms are beautifully decorated, two have jacuzzi tubs for two. All with digital cable TV and vcr. Massage therapy,Reiki,and our new biofeedback computerized healing machine sessions are available. Workout room with boflex. Full organic breakfasts.
BedandBreakfast.com gift cards are the perfect gift for anyone who needs a relaxing getaway.
Score Breakdown based on10 Reviews
Write A Review
on April 22, 2015
Didn't want to leave. The view from the pool deck is amazing. Claudia & Rolan are great hosts. Must try the blueberry pancakes. I'll be back.
on April 15, 2015
Claudia's Enchanted Manor is a sybaritic haven. Warmth, comfort, wealth and abundance. Everything is green, ecofriendly and organic. you leave feeling lighter, happier, brighter and indulged. Cannot recommend it highly enough.
on August 02, 2011
The hosts Claudia & Roland could not be more attentive to our stay and needs. A great experience.
on March 11, 2009
Beautiful accommodations. Beautiful people.
on February 12, 2009
We stayed in a spacious suite, quiet, comfortable, the furnishings invite comfort and peace. Claudia and Roland are wonderful hosts. We would highly recommend this lovely B&B!
on February 12, 2009
A comfortable, warm and peaceful ambiance. Friendly, clean, and great setting.
on August 29, 2008
This B&B provided a relaxing, calm and luxurious getaway for us. Claudia was a wonderful host and the house is warm, welcoming and beautiful. The pool was perfect and everything was taken care of.
on August 27, 2008
A definite way to take a break from the hecticness of your average day.
on August 27, 2008
Beautiful home, wonderful hosts. Would totally recommend for a quiet peaceful getaway!
on September 18, 2007
Enchanted Manor is exquisitely beautiful - - the decor, the gardens, and the hosts. Every moment was more than comfortable, relaxing, and so pleasant. The friendliness and generousity of Claudia and Rolan is like being with family. Almos forgot; breakfast is delicious and hearty!
View More Reviews
Albany, NY (ALB-Albany County) 60 miles
see website
Antique Shopping,
Art Galleries,
Gourmet/Fine Dining,
Historical interests,
Live Music,
Movie Theater,
National/State parks,
Nature area,
Outlet Stores,
Performing Theater,
Picnic area,
Religious Services,
Ski area
BBQ grill, CD player, Computer, Internet Service, Telephone, Washer/dryer, Wedding Facilities ,
gym, massage therapy, pool
Air conditioning, Alarm clock, Bath robes, Ceiling fans, Fireplace, Hairdryer, Hot tub/whirlpool tub, Radio, Satellite/Cable TV, TV, VCR
Bicycle rental, Cross-country ski trails, Downhill Skiing, Golfing, Hiking trails, Horseback riding, auctions, Shopping, Sight-seeing, Ski rentals, Snow shoe trails, Swimming, Swimming pool, Tennis courts, Whitewater Rafting
Claudia and Rolan
health and healing, yoga, meditation, dance
Restrictive , Vegetarian
Regular Rate:
$200 now
(20% Savings:)
Gorgeous B&B in Woodstock, beautifully decorated on 8 private acres. Jacuzzi tubs, salt water pool, pond with waterfall, full healthy breakfast.
Please contact the inn directly at +1-845-679-9012 to redeem this deal.
expires Wednesday
$170 per night
Innkeepers: Claudia and Rolan
Guests: 2
Simple! BedandBreakfast.com guarantees you're getting the best price on your bed and breakfast stay when you book your room on BedandBreakfast.com. Within 24 hours of your booking, if you find a better price for the same room on the same date(s) at the same B&B, BedandBreakfast.com will refund the difference to you!
The Best Price Guarantee covers any Bed and Breakfast booked online through BedandBreafkast.com. The Bed and Breakfast must be booked using the Book It button on BedandBreakfast.com in order to qualify.
Just call 1-512-322-2710 within 24 hours of booking and give our customer support representative the details on the lower price you found.
The BedandBreakfast.com Best Price Guarantee ensures that when you book and pay for your reservation online through the BedandBreafkast.com website, you are booking your room at the lowest available price. If you find a lower price for the same room on the same dates at the same B&B, and contact us within 24 hour of making your booking, we will refund the difference in price to you. This guarantee does not apply to bookings made over holidays and/or for special events. All requests are subject to verification by BedandBreakfast.com. BedandBreafkast.com will not accept screenshots or other purported evidence of a lower price that cannot be independently confirmed by BedandBreakfast.com personnel. Nor will BedandBreakfast.com verify any requests that it believes, in its sole discretion, is the result of a printing or other error or is made fraudulently or in bad faith.


text:
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Daily Sales Up to 70% OFF Limited-time deals start every day at 12 pm ET.
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Product Information:
Vector Pro™ II provides a clean look with its new cable management capabilities. The mount also features a ceiling plate, ball and socket design, multidirectional positioning (roll, pitch, and yaw) for proper image alignment and complete Armor Lock™ security at every connection point. This projector mount is perfect for use in classrooms, boardrooms, hotels, bars and restaurants or anywhere a projector needs to be mounted. The Vector Pro™ II can be flush mounted, mounted using extension column, mounted using thread rod or wall mounted.
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Included with purchase ($20 value).
Additional Documents
Weights & Dimensions
|Mount Type: Ceiling mount
|Finish: Black
|Hardware Finish: Zinc
|Powder Coated Finish: Yes
Primary Material: Metal
|Additional Materials: ABS
|Hardware Material: Steel
|Number of Items Included: 1
|Scratch Resistant: Yes
|Rust Resistant: Yes
|Corrosion Resistant: Yes
|UV Resistant: Yes
|Fade Resistant: Yes
|Chip Resistant: Yes
|Non-Skid: Yes
|Non-Toxic: Yes
|Legs Included: No
|Adjustable Height: Yes
|Articulating Arm Included: No
|Quick Release: Yes
|Cable Management: Yes
|Universal Mount: Yes
|Drop Mount: Yes
|Ceiling Recessed: No
|Motorized: No
Swivel: Yes
Tilt: Yes
Rotation: Yes
|Locking: Yes
|Compatible Mounting Surface: Wood stud; Solid concrete
|Weight Capacity: 50 Pounds
|Commercial Use: Yes
|Country of Manufacture: China
|Assembly Required: Yes
|Installation Required: Yes
|Additional Parts Required: No
|Product Warranty: 5 Year for parts
UL Listed: Yes
|cUL Listed: Yes
|RoHS Compliant: Yes
About the ManufacturerPeerless Industries has been the leader in the audiovisual mounting industry for over 40 years, providing innovative mounting solutions that focus on quality, ease of installation, and functionality while providing an aesthetically pleasing look and ultimately the best value. Peerless offers the most comprehensive line of video and audio mounts and installation accessories for residential and commercial markets. Peerless' line of equipment includes a broad selection of wall and ceiling television mounts and accessories, LCD mounts, plasma mounts, projector mounts, AV carts and more.
Peerless mounting solutions help display technology users maximize investment with a comprehensive line of mounts and accessories. Commercial and residential installers trust Peerless product for installations in schools, universities, offices, healthcare facilities, hotels, sports arenas, training facilities, retail environments as well as homes. Peerless products provide a variety of audiovisual mounting solutions for any home application. Functionality, safety, security and reliability bundled with an aesthetically pleasing design, and the Peerless peace-of-mind with every mount, provides an unbeatable value.
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We want you to love it. If you don't, you may return your purchase within 30 days of delivery. All you need to do is visit our Self-Service Return Center for instructions. Return credits are issued within 24 hours once the item is received and inspected. Please note that shipping charges are non-refundable. The cost of return shipping will be deducted from your refund. 30-Day Return Policy
Overall Rating
Based on 11 reviews
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Perfect for my needs Lizunia from Costa Mesa, CA on 02/06/2011 – Verified Buyer
I bought this mount to mount my DVR and my cable box to my outside patio cover. I needed a mount that I could MacGyver to use for my needs. Although it was a little expensive, it worked perfectly. I was easy to install. And I liked that the mount could be separated from the housing so that it made it much easier to install without the housing getting in the way. Then i just loosened the mount pole and swiveled it on to the housing. I originally didn't get an extension pole becasue I wanted the unit to be up high, but I didn't consider putting in the DVDs, so i have bought and extension pole and now it is perfect as it sits just above the wall mounted TV. People who say that is it hard to install must be mechanically challenged.
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Works perfect for ceiling mounting CM from GA on 06/15/2014 – Verified Buyer
Our projector slid right in. easy assembly, looks great. also purchased the extension rod---connected easily. Very sturdy
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Perfect Chris from Allendale, NJ on 03/04/2013 – Verified Buyer
Just what I needed for my viewsonic pro8200 after one of the cheap mounts I bought before this broke. The cheap screw snapped off in the pj leaving me looking for a clamp design. This is great quality, and offers extension arm as an extra accessory.
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Great Mount BobR from Lincoln, RI on 12/07/2010
This mount was selected due to its flexible design and ability to fit the projector perfect. The sliding sides and top make it perfect for several different projector sizes and shapes. The swivel ball on top with a one screw lock made it very easy to adjust once it was mounted. All components were a very high quality.
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i would buy this again Perfect geterdone from ky on 08/01/2011 – Verified Buyer
fits like a glove, easy installation,
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