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Amanda's Closet
- Filters:
Brand new never worn New Balance 515NWT
$60 $70
Comes with mint green laces and white. Colors are navy peach mint and yellow. SO cute. Size is 5.5 but fits like a 6.
Little girl Hunter Wellies.
$26 $75
Size 8 toddler. Loved by my little girl with some scuffing but in great shape. Color is a dark gray with a pink bottom.
Kids size 10 Wellies
$21 $70
My son used these for a season. Love the army green. They have some scuffing but are in great shape.
New with tags Neimans long sleeved black crop topNWT
$40 $140
Gorg on trend crop. Amazing with high waisted Anything. Shirt has stretch
Banana Republic SUPER flattering maxi
$20 $85
The stripes on this make lumps and bumps fade far away. Stretchy and amazing
Citizens of Humanity low waist full leg
$35 $220
Well loved Citizens jeans. Some wear on hem. Really beautiful cut.
J Brand high waisted skinnies. Maria Size 27
$70 $220
Like new high waisted J Brands. The gold standard of skinnies. In amazing shape. Not hemmed. Style Maria color Avalon
JCrew short sleeved denim button down.
$25 $98
Awesome on trend shirt. Super for layering. Great shape barely worn
JCrew printed blue heather tee. Sporty cut
$15 $54
Super cute tee under a blazer. So soft and great fit.
Urban Outfitters oversized gray blazer.
$20 $98
Super well fitting slouchy blazer size xs. Love this thing. Great condition. A bit of discoloration on the inside lining. Not visible from outside
Stunning navy and white maxi. Loft petites
$25 $98
Size 2p chiffon maxi. Such a stunner with red accessories. Never worn
Awesome J Crew 3/4 sleeve shirt
$20 $48
Black and cream fitted tee. Looks very cool with everything.
Coral fitted JCrew painters tee
$15 $39
Great color shirt. Looks hot w army green. Has a vintage worn look.
JCrew fitted painters tee
$15 $45
Great color stripe. Vintage feel. Great cut great condition
Amazing retro mini dress
$15 $27
F21 dress that is surprisingly well made and looks expensive. True to size. Great party dress
JCrew oversized button down. Except for cool girls
$20 $112
Small check blue print men's button down in xs. I bought it for a cool oversized look. Perfect condition.
Super cool puma jacket. Hard for me to part with.
$35 $112
So on trend. Super great color. Great shape. Some fading around the collar. Otherwise perfection
THE brand of T Shirts in LA. LNA awesome white tee
$20 $90
Cute v neck fitted tee
JCrew sporty tee. Great basic
$15 $54
Cute white tee with strip detail. Great fit
Anthropologie tribal print shirt. Amazing
$15 $85
Amazing stitched detail in silver and black. Great with skinnies or leggings. Total statement.
gap xs petite bright blue maxi
$10 $65
Can be worn off the shoulder or as cowl. So cute. Dressed up or down
Super cute Free People sweater
$30 $120
Sheer Free People chevron billowy sweater. It's a great cut
White BR blazer. So fresh
$15 $98
Petite blazer had a great look a fit. Some discoloration around collar. Not visible when on.
J Crew peach button blouse Boho style
$18 $98
So cute hippie blouse. Amazing color with covered buttons. Size 2
Paige Skyline ankle peg size 27
$40 $185
Like new ankle peg jeans purchased from Anthropologie. Super cute for sandals
Hot Pink satin 4" platforms by RSVP
$20 $85
Worn once for 2 hours. Totally perfect. Looks great with a black dress.
Paige Skyline Skinny size 27
$30 $158
Rarely worn Paige jeans. Lots of stretch. Fountain wash. Lightweight for summer. Awesome jeans.
Well preserved authentic Gucci Horsebit Hobo
$8000000 $1800
Guaranteed authentic Gucci with dust bag. Used briefly as a laptop bag then put into preservation and storage. Purchased at Gucci store for $1800. This is the larger size of the horse bit bags. On the fence about selling it because its so beautiful and in near perfect condition. Really great bag.. Price is negotiable and would prefer Paypal
Zara NWT pointy nude stilettosNWT
$50 $80
Sold out online immediately. Never been worn. Perfect edgy shoe to go with everything
Jcrew Cream wool cardigan with leather buttons
$45 $87
Versatile and classic cardigan. Falls to low waist. Bag is also for sale. See listing in my closet. Reserved bundle
New Gap Perfect Boot Jean
$30 $70
Now in stores. Never worn. Size 27 ankle. http://m.gap.com/product.html?dn=gp673946&sdReferer=&kwid=1&pid=673946&referrer=http%3A%2F%2Fwww.gap.com%2Fproducts%2F1969-perfect-boot-jeans-P673946.jsp&sem=false&locale=en_US
lightweight jcrew leopard cardigan with satin trim
$45 $120
So stunning and barely worn. Gorg over a dress or with a tee and ripped jeans
Marc by Marc Jacobs pendant
$45 $158
Worn a few times. Super cool red and blue and gold pendant. Tiny mark on the blue area pictured. Otherwise perfect
Oversized black leather grommet clutch
$60 $250
Super cool clutch. Never been used. Only selling because its from an ex friend and I want no reminder. Seriously nice leather, great size. A total rock star standout
Amazing DVF mod 60s dress
$80 $350
Beautiful stretch fabric that has a bounce when you walk. Super cool vintage cut. Flattering on a tummy
High fashion Cavalli gown
$85 $375
Made in Italy stunning Cavalli gown with unique zebra bodice. Wore once and got a million compliments. Fabric has some stretch
Jcrew long nautical stripe tee
$15 $38
Lots if stretch nice and long. Looks great with yellow shorts posted
Seven jeans in trouser flare Ginger style.
$45 $180
Rachel Zoe's fave style. Higher waisted and wide flare. Light weight denim. 30" inseam
Kate Spade red flats
$10000 $220
Awesome suede red little driving flat with silver detail. Barely worn
Rachel Pally never been worn maxi with deep V
$50 $180
Beautiful plum color in thick stretchy fabric. Flows beautifully as you walk. Super long for a tall girl but hemmable as well.
Amazing anthropologie zip peplum
$1000 $85
Flattering blouse with two tone navy and lilac. Zips up front with tie on the back. Beautiful silky material
Stunning anthropologie ruffle blouse
$1000 $185
Pinky lilac lightweight ruffle blouse. Total showstopper.
Adam Lippes pink cocktail dress
$1000 $350
Sexy girly dress in a heavy linen for summer. Worn once to a wedding. Best for smaller bust
Lilly Pulitzer hot pink v neck
$1000 $87
Fitted stretch looks so cute layered. Great conditioned barely worn
Nordstrom back zip racer back tank
$25 $89
Coral silk lined gray tank with back zipper. Super cool flowy shirt. Like new
Kimchi & Blue sheer boho top
$25 $58
Beautiful color sheer summer top. Wear with ripped jeans
Hipster Philly tee super soft
$10 $30
Love love this. So soft and cool
SOld Jcrew high rise skinny cord in blue
$10000 $98
Paradise blue perfect condition cords. Looks so cute with heels or rolled with flats
Loading more..
Recent Brands


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Best Bets: From Sailfest to Shakespeare with Much in Between
There's lots of summer fun to be had this weekend from Sailfest in New London to Riverfest in Hartford and a lot in between.
CT Attempts to Improve Diversity & Police Monitoring to Avoid Race-Related Conflict
Nationally, incidents involving police and race have sparked outrage, and even in some cases riots—how are Connecticut officials addressing the Issue.
Vinny's Deli in Wallingford Makes the Claim for Best Roast Beef in Connecticut
We love a great roast beef sandwich; we also love the U.S. of A. And when you can cover two loves with one bite, it's hard to pass up. Enter the roast beef sandwich at Vinny's Deli in Wallingford, a.k.a. "The King of Roast Beef." For a claim that bold, you would hope the sandwich is something special. The good news is that in this case, it is—a moist, tender, flavorful delight, due in large part to a single-minded dedication to creating arguably the best roast beef sandwich in the state. "It's all we do," says Vinny Iannuzzi, who has been running his eponymous deli—which has an enormous roast beef sandwich sculpture across its front—for 33 years. "No dinner, no slushie machines. We just make sandwiches."...
Say No to Knockoffs: When the Wedding Dress Seems Too Good to Be True
With wedding gowns costing thousands of dollars, many brides are searching for cheaper options, one of which is knockoff dresses ordered off the internet. Can a knockoff hold its own against a designer gown? The Connecticut Bride staff ventured to find out.
Pinup Calendar Raises Money and Awareness to Fight Domestic Abuse
Connecticut Calendar Girlz annual pinup calendar raises money for the Interval House, a Hartford-based anti-domestic violence resource.
Weekend Best Bets: Boom Box Parade, Heart and Celebrate America
It's a long holiday weekend and there's plenty to do around the state.
Adventure Rooms in Middletown Bring Escape Room Craze to Connecticut
The hot new gaming trend is like a video game experience come to life.
Don't-Miss July Craft Beer Events
This month will see a host of beer events, both big and small, that are worthy of attention.
Christina Perri's Girls Night Out with Colbie Caillat & Rachel Platten at Mohegan
Singer/songwriter Christina Perri brings her Girls Night Out Tour to Mohegan Sun with fellow female powerhouses Colbie Caillat and Rachel Platten on July 10.
The CT Files: 'America's Lost Byron,' Fitz-Greene Halleck
The forgotten career of a local literary "giant."

subset: commoncrawl


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Restaurants in Brazil
Chef Atala's new casual restaurant. The adobe-hued Dalva is all about sharp updating of grandmotherly regional dishes.
A perfect lunch destination for Ipanema shoppers. In the tastefully underdone, concrete-floored space, genial young staffers serve Italian standards both traditional (pizza alla Margherita) and adventurous (tramezzini with salmon tartare).
Tuck into the ultra-cheesy pies from the infernal wood-burning oven and the pleasantly oily Calabrian sausage bread.
The restaurant, which sits high above Cambury Beach on a forested hillside, is the current hot spot; Brazilian-accented Italian cuisine (conchiglia del mar—a cheesy, creamy seafood casserole baked in a seashell) fuels the chic diners, who spend the rest of the evening sipping on caipirin
Order the Super Burguer com pimenta at this burger haven.
Using Brazilian ingredients like cassava and açaí, chef Claude Troisgros (a generation of the family famous for attaining Michelin stars) produces French cuisine that's considered some of the best in Rio.
Maria Sylvia Esteves Calazans Luz came from São Paulo to Trancoso in 1974 at the age of 32. She was soon cooking elaborate meals for guests at her beachfront cottage on the Praia do Espelho.
The local intelligentsia frequent Filial, where schoppe (draft beer) flows into the wee hours.


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it's no secret: we love the nightcap lace bell bottoms! there are so many ways to style them, and our store girls are pros- check out these lovely pics of them rocking our fave pants!
katie, mit at fp san jose, wears them with a strappy cami, ruffle hem cami and denim jacket and hat for a cute casual look.
here's one of our denver sales associates, wearing them with the tape yarn beach sweater! love.
one of our customers, who dressed them up with a button-down crop top and hat!
i love this outfit! one of our nyc girls styled the pants with the textured bottom trapeze tank and archipelago fringe tank.
from boston: sales associate stephanie is rocking the american flag scarf– she's a hippie child and a dreamer! she looks super cute and casual, showing you don't have to be over the top, and can totally pull them off everyday. and becka, the soon to be natick ASM, is wearing the grey in an adorable beach style with the pintuck lace square neck top!
and from houston galleria – simple and stunning with a tank top and aztec seed bead necklace.
thank you so much ladies! find the nightcap lace bell bottoms here.
- -Apr 03, 2014, 12:27AM
Hi! Do you use Twitter? I'd like to follow you if that would be ok. I'm absolutely enjoying your blog and look forward to new updates.
- -Jun 12, 2011, 8:43AM
this is AWESOME!! i love these pants i haven't seen them before! have to check them out NOW... www.dragonnfruit.com


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For a compliance program to be truly successful, employees and leadership alike must know that it exists, understand it, support it, and live by it. Most successful programs involve increasing communication with employees in seven core areas:
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2. Company Commitment: Establishes a trustworthy work environment
3. Non-retaliation: Improves culture
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7. Consequences: Makes compliance personally relevant
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text: Gossip. New York, N.Y. Schreiber Ellen Schreiber, Ellen. en Fiction Vampire kisses 2003 2003 51306186 1st ed. Juvenile works Sixteen-year-old Raven, an outcast who always wears black and hopes to become a vampire some day, falls in love with the mysterious new boy in town, eager to find out if he can make her dreams come true. Unknown value: ejh 9780060093341 006009334X Vampires. Interpersonal relations. 9780060093358 0060093358 Vampires nyu Interpersonal relations Young adult fiction. Katherine Tegen Books/HarperCollins Publishers Gossip


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Personal Campaign
Veneta is gathering 10 signatures to
Stop Dolphins Slaughters In Taiji Cove Japan
Murders in Taiji Cove
Veneta's progress
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10 Veneta's goal
Murders in Taiji Cove
Does it not irk you when your local animal welfare accepts stray dogs & cats only to euthanize them later!!
97% of Americans support tough new anti-corruption laws. Together, we can and will force our government to Represent Us!
Puppy Doe was brutally tortured and in her memory we want to help animals.
Sign this petition to pressure the government of Argentina to release Arturo
Prevent animal abusers from adopting again!


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xbone-reto... Retoucher
OguzhanPhotographer Male İstanbul, İstanbul, Turkey
My Website: facebook pageMy MM URL: http://www.modelmayhem.com/ucusankareler
Mayhem # 3249464
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Omar Berk TRGT Model
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Meltem Model
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View divorced Singles in Maxwell
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Lone Star State of Texas. Match.com personals brings singles together in Texas. Why is Match.com the most popular way in the world to find love on your terms? Is it because we've inspired twice as many marriages as any other site in the world? Or is that every year, hundreds of thousands of people find love on Match.com? Or is it that no matter how complicated love can be, match makes finding that special someone in Maxwell so simple? Addicted to love? Get your fix through thousands of Maxwell profiles all looking for love.
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September 1, 2010
Toy Story
A recent study suggests that shelters can increase the chances of their cats getting adopted by putting toys in the cats' cages and housing them at eye level.
September 1, 2010
The Purebred Paradox
The quest to create the "perfect" dog may be contributing to a genetic health crisis among purebreds. A lack of genetic testing, mating of closely related animals, and breed standards that encourage extreme features over health have been blamed for a host of health problems facing scores of breeds today.
September 1, 2010
Shelter Medicine: Canine Distemper Virus: Anatomy of an Outbreak
Canine distemper virus can easily enter a shelter, is tricky to diagnose, and may wreak havoc on a shelter's dog population. A veterinarian and a shelter official discuss the policies and procedures that helped a shelter in West Kennebunk, Maine, cope with an outbreak.
July 1, 2010
Collars and Sense
It's often said in the sheltering field that a collar and identification tag are a lost pet's best chance of returning home. That's especially true for felines: Only 2 to 5 percent of the millions of homeless cats entering shelters each year are reunited with their families.
July 1, 2010
Spay it Ain't So
Study on ovary retention and longevity shouldn't affect shelter spaying practices.
July 1, 2010
Off Leash: Bright is the New Black
Marti Houge's bright bandanas give color-challenged pooches a better chance of catching someone's eye.
July 1, 2010
Shelter Medicine: Kittens: Coming Soon to a Shelter Near You
During the spring and summer'also known as "kitten season" to shelters and rescues'pregnant cats, nursing mothers, and kittens overwhelm facilities across the nation. Shelters have to take extra measures to protect young kittens from exposure to germs, and must provide them with a series of vaccinations, plus high-quality nutrition and proper deworming, to keep those mewing fuzz balls healthy.
July 1, 2010
Mouthpieces: Love Your Cat? Then TAG Your Cat!
In this issue, Mouthpieces helps you communicate to pet owners the importance of outfitting their precious feline companions with a collar and tag.
May 1, 2010
Off Leash: Meet the REAL Slum Dogs of India
During a trip to India, British photographer Eloise Leyden stumbled upon Tree of Life for Animals, a nonprofit that cares for former street dogs at its sanctuary in Pushkar. The people (and canines) she met there played a role in the creation of her photography book, Slum Dogs of India.
May 1, 2010
Survey Says ... Get a Cat!
The Morris Animal Foundation surveyed non-cat owners about their perceptions of cats to gain insight into what may be preventing them from finding those homes. The results show that the next wave of cat owners may come from unexpected segments of society.


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No headline - let-pineros
John Pineros, Golden Gate
Can you help me?
I am an upcoming junior at Golden Gate High School who has been striving for my dream of becoming a cardiologist. I'm very driven, and I've made many sacrifices such as denying myself free time to work on homework and projects, and also to work on community-service hours and fundraising for, in this case, the National Youth Leadership Forum.
I was recently invited to a prestigious medical summer program this June. The program lasts for 10 days and takes place in Washington, D.C. I will be learning about medical situations hands-on.
The cost of the program is $2,645, plus traveling expenses. I applied for a grant from the program but only received 40 percent of the total amount due. At this point, I'm exhausting all possibilities as I search for financial aid to make it possible for me to attend this program. My family is in a very tight position; my father has recently lost his long-sustained job as a truck driver, and this has left us with very little income. I am willing to work for any support and I am willing to help in any general services needed, such as lawn care, pet walking/washing, car washing and/or house cleaning.
If you can be of aid to me, please contact my sponsor, Veronica Perinon through her Golden Gate High office number, 239-377-1680; the school office number, 239-377-1600; or the school fax number, 239-377-1601.
I am aware that the economy is in a horrid state right now; however, any financial support would be very much appreciated. Thank you for your consideration.
Subscribe to the Naples Daily News
Need Help? Call us at 1-888-878-6842.
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I have a 75 gallon with stand, 30 gallon and 10 gallon. I want to sell everything including all accessories. Want to move and don't want to relocate them. They have lids, lights and a lot of other including the python for cleaning. I want to sell it all. Tank has been drained and ready to go. Serious inquiries only. Call after 5 pm 847-275-8246
Oct 3 -
Chicago :
Dundee - Other pets
PICK UP ONLY !!Nice tame pair of tame jungle Carpet pythons proven breeders.they are nice and healthy pounding frozen thawed rats.the come with 150 gallon tall with custom lid and about $300 in lamps,branches,bowls ect.very nice display for your house,reptile room etc.will not separate anything this is about a $1500 value but $600 takes it all.call or text 773-619-1039 anytime
Sep 14 -
Chicago :
Chicago - Other pets
Up for adoption I have a few offspring from the father pictured. They are eating small crickets,meal worms,bean beetles and fruit flies. Re-homing fee of 225$ or best offer. I do welcome trades for goods that are equal or greater resale value. They will come with 1 month health guarantee and life time support.
Age is between 1.5-4 months but I do have older ones for sale as well. Please ...
Sep 11 -
Chicago :
Oak Lawn - Other pets
I have 3 baby Ball Pythons with tank and light .they all eat really good . about 2 to 3 months old.
Sep 10 -
Rockford :
Mendota - Other pets
SELLING A BABY SPIDER BALL PYTHON . ASKING FOR $125.00 or BEST OFFER . PLEASE SEND ME A TEXT ONLY AT 708-541-3006
Sep 2 -
Chicago :
Oak Forest - Other pets
Keno is a fabulous ball python that is a great eater and not aggressive at all. He is at 5 foot.
Aug 24 -
Central Illinois :
Loraine - Other pets
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Our friends say we have a lip balm addiction (we've got 'em in our jacket pocket, glove compartment, and on our bedside table, to name a few places), but we like to think we're just prepared. We'll be stashing one of each of these lip balms in said locales, including prime purse real estate—they're packed with moisturizing ingredients like shea butter, and as a bonus, each is infused with one of five pretty hues, ranging from a flattering nude to a bright raspberry.
I have to put this type of product on my lips because they dry out real bad. I purchased this because of the good reviews and they are right. I don't like dark colors on myself so I am very happy with my choice. I know you will like it too. Just go with the lip colors you like and enjoy.5


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|Publication number
|Publication type
|Publication date
|17 Aug 1937
|Filing date
|26 Oct 1934
|Priority date
|26 Oct 1934
|Publication number
|Cicero William A
|Original Assignee
|Cicero William A
|Export Citation
|BiBTeX, EndNote, RefMan
|Referenced by (27), Classifications (7)
|External Links: USPTO, USPTO Assignment, Espacenet
1937. I w. A. ClCERb 2,0901% 1 I SHOE RACK Original Filed Oct. 26, 1934 2 Sheets-Sheet l W. A. CICERO Aug, 17, 1937.
zwmos SHOE RACK Original Filed Oct. 26, 1934 2 Sheets-Sheet 2 Patented Aug. 17, 1937 UNITED STATES OFFIQE Application October 26, 1934, Serial No. 750,214 Renewed December 4, 1936 3 Claims.
This invention relates to furniture and more particularly to racks for holding shoes in convenient position for removal when required and to prevent them being knocked about on the floor 5 of a dressing room. The invention seeks to provide a shoe rack which may be secured upon a door or wall and will extend therefrom to support the shoes or may be collapsed into a flat condition against the wall when its use is not required. The invention is illustrated in the accompanying drawings and consists in certain novel features which will be hereinafter first fully described and then more particularly defined in the appended claims.
In the drawings:
Figure 1 is a perspective view of a shoe rack embodying the invention and arranged for use.
Figure 2 is a section on the line 2-2 of Figure 1.
Figure 3 is a similar view showing the rack in collapsed condition.
Figure 4 is a front elevation of one end of the rack in its collapsed condition, the line 3-3 in this figure indicating the plane of the section shown in Figure 3.
Figure 5 is an enlarged detail section on the line 5-5 of Figure 2.
Figure 6 is a perspective View showing a modified form of the shoe rack.
Figure '7 is an enlarged detail perspective on the line 1'l of Figure 6.
Figure 8 is a detail perspective of a modification.
In carrying out the invention, there is provided a pair of standards or bars I which are provided q with openings 2 therethrough so that screws or similar fastenings may be inserted to secure the bars upon a door or wall. The outer edges of these base bars are stepped, as clearly shown at 3 and 4 in Figure 1, and upon the outer edge of each bar are pivoted links and supporting arms 5 and 6, as shown. It will also be noted that in the illustrated form of the invention, the links 5 and the supporting arms 6 are duplicated so that there are provided two racks one above the other for holding the shoes. The arms 6 are pivoted to the base bars immediately under the steps or shoulders thereon and at their outer ends are engaged by rods l which support the toes of the shoes. Near their upper pivoted ends said arms are recessed, as indicated at 8, and bars 9 are secured in said recesses and extend between corresponding arms so as to support the heels of the shoes, it being understood that the breasts of the heels will rest upon the upper or rear 55 edges of the bars and the soles of the shoes will extend across the bars so that the toes will rest upon the rods 1. It will also be noted that the arms are downwardly inclined from the base bars, as shown most clearly in Figure 2. The bars are provided with a plurality of openings I0 5 therethrough and when the rack is set up for use the rods I extend through the outermost of said openings. The links 5 are provided at their free ends with openings which receive the ends of the rods i so that the links and the arms will be pivl0 oted together and will be interlocked by the rods so that dropping of the same when extended for use will be prevented. The rods are preferably equipped with washers and securing screws, indicated at H, which bear against the outer sides 15 of the respective links and thereby prevent accidental removal of the rods. By engaging the rods in difierent openings it, the inclination of the arms and the links may be varied to suit the desires or tastes of the user. The lower links 5 are provided each with an opening 52 therethrough at a point near the pivots thereof and arranged so that when the racks are collapsed said opening will register with the uppermost or innermost opening it in the upper arm. When the racks are to be collapsed, the rods 1 are withdrawn and after the racks have been collapsed, the upper rod 7 is inserted through the openings l2 and the openings l0 registering therewith as well as through the openings in the ends of the links which at this time will be registering with the openings l2 and the said openings I0 so that the upper and lower links and the upper arms will be locked together and held against possible accidental swinging out from the wall or door to the possible injury of persons in the room. The openings in the ends of the lower links will be brought into alinement with the uppermost or innermost openings lit in the lower arms when the arms and links are swung downwardly and the rod 7 is then engaged through said alined openings so that the lower arms will be locked in the collapsed condition, as will be understood upon reference to Figures 3 and 4.
The rack is composed of few parts so that it will not be heavy and may be easily set up and manipulated. It may be produced at a low cost and when collapsed will be very compact and when set up will firmly support any number of shoes up to the limit of its capacity.
The rack which has been described and is shown in Figures 1 and 5 is constructed of wood but a rack may be constructed of metal and in Figures 6 and '7 I have shown such a rack. Angle bars 20 are provided and are connected by braces 2| so that they will be maintained in spaced relation and these angle bars are provided with openings 22 whereby they may be secured upon a Wall or door. In the forwardly projecting webs of the angle bars are vertical slots 23 which receive bolts 24 having wing nuts 25 mounted upon their inner ends. Arms 28 are pivotally mounted upon the bolts 2t and are held thereon by the heads of the bolts, as will be understood upon reference to Figure 7, and links 21 are pivoted upon the outer sides of the base bars 20 adjacent the upper ends thereof and extend outwardly therefrom to be pivoted at their free ends to the arms 2%.
Slots 28 are provided in the arms E6 and pivot bolts 29 carried by the links are engaged through said slots and held by set nuts at the inner sides of the arms so that the inclination of the arms may be varied to suit the user. In termediate their ends, a cross bar 30 is secured to and ext-ends between the arms, and at the free ends of the arms a rod 3! is provided. The shoes are supported at their toes upon the rod 3| and the heels engage over the bar 30 so that the shoes will be firmly supported.
As shown in Fig. 5, the washer H at one end of the rod l is larger than the hole through which the rod passes so that it serves as a stop. The washer 32 at the opposite end of the rod is small enough to pass through the opening but is fastened eccentrically. When inserting the rod, the washer 32 is turned as shown in full lines in Fig. 5 and that end of the rod is inserted through the openings, the endwise movement of the rod being arrested by the Washer H impinging against the rack and the washer 32 being then turned to the position shown in dotted lines in Fig. 5 thereby preventing endwise movement of the rod in either direction.
The metallic rack shown in Figure 6 accommodates only a single row of shoes but a double rack may be made of metal. In such a rack, as shown in Figure 8, the back or base of the rack will consist of side bars 33 and cross bars 3 3 integrally connecting the upper and lower ends 1. A shoe rack comprising base bars adapted to be secured upon a fixed support and having their outer side edges provided with stepped shoulders spaced downwardly and inwardly from each other, arms and links pivoted to the outer side edge faces of the base bars under the shoulders and detachably connected at their free ends, bars extending between pairs of arms and secured to the arms between the ends thereof, said bars extending between the arms in spaced relation to the free ends thereof to support the heels of shoes, and a rod engaged with the outer ends of the arms and extending therebetween to support the toes of shoes.
2. A shoe rack comprising base bars adapted to be secured to a fixed support and having shoulders spaced vertically from each other and offset transversely of the bars, arms pivoted to the base bars under certain of the shoulders, the arms being provided with series of openings at their free ends, links pivoted upon the base bars under other shoulders and each having an opening at its free end, and a rod extending between front ends of the arms and engaged in the openings at the ends of the links and in selected openings at the ends of the arms.
3. A shoe rack comprising base oars adapted to be secured on a fixed support, upper and lower arms pivoted to the edges of said base bars, upper and lower links pivoted upon the base bars above the respective arms and provided with openings at their free ends, the lower link having an opening therethrough between its ends and the arms having series of openings at their free ends, and rods engageable in selected openings in the ends of the arms and in the openings in the ends of the links, the arms and links swinging downwardly when the rods are removed and the openings in the ends of the upper links register-- ing with the upper opening in the lower link and with openings in the upper arms when the parts are in the collapsed condition whereby the insertion of a rod through said openings will lock the parts together and the openings at the ends of the lower links will aline with openings in the lower arms in the collapsed condition whereby the insertion of a rod will lock the lower links and the lower arms in the collapsed condition.
|Citing Patent
|Filing date
|Publication date
|28 Apr 1950
|7 Apr 1953
|Henrietta Derman
|Garment hanger rack for doors
|24 Sep 1956
|24 Dec 1957
|Lasky William N
|Chinning bar attachment
|16 Jan 1956
|3 Mar 1959
|Gadget Of The Month Club Inc
|Wardrobe fixture
|25 May 1962
|22 Dec 1964
|Scott Rono W
|Detachable, collapsible combination rack for foot locker and shoes
|17 Jan 1989
|26 Jun 1990
|Bobeczko James D
|Sports equipment rack
|10 Apr 1996
|9 Dec 1997
|Lynk, Inc.
|Hanging shoe rack
|5 Mar 1996
|16 Feb 1999
|Suncast Corporation
|Storage apparatus for sporting goods
|29 Jan 1998
|29 Jun 1999
|Suncast Corporation
|Sports shelf
|8 Jan 1999
|31 Oct 2000
|Lynk, Inc.
|Hanging rack for sports equipment
|20 Aug 1997
|28 Nov 2000
|Lynk, Inc.
|Clothes hanger with sliding hooks
|24 Aug 2000
|15 Oct 2002
|Lynk, Inc.
|Hanging modular storage unit
|28 Aug 2000
|15 Oct 2002
|Lynk, Inc.
|Hanging shoe rack with double loop shoe retaining arrangement
|18 Aug 2000
|18 Mar 2003
|Lynk, Inc.
|Over-door shoe racks
|3 Jul 2002
|28 Oct 2003
|Lynk, Inc.
|Over-door shoe racks
|20 Aug 2002
|7 Sep 2004
|Lynk, Inc.
|Wooden shoe rack construction
|3 Jul 2002
|21 Sep 2004
|Lynk, Inc.
|Over-door shoe racks
|8 Sep 2003
|12 Apr 2005
|Lynk, Inc.
|Over-door shoe racks
|8 Sep 2003
|9 Aug 2005
|Lynk, Inc.
|Over-door shoe racks
|8 Sep 2003
|31 Jan 2006
|Cooper Vision Inc.
|Ophthalmic lenses and compositions and methods for producing same
|8 Sep 2003
|4 Apr 2006
|Lynk, Inc.
|Over-door shoe racks
|8 Sep 2003
|11 Apr 2006
|Lynk, Inc.
|Over-door shoe racks
|29 Dec 2005
|3 Jul 2007
|Merrick Engineering, Inc.
|Over the door hanger system
|8 Sep 2003
|19 Aug 2004
|Klein Richard B.
|Over-door shoe racks
|2 Aug 2004
|6 Jan 2005
|Lynk, Inc.
|Wooden shoe rack construction
|14 Nov 2003
|19 May 2005
|Hosilyk Rodney E.
|Ribbon and wrapping paper storage and dispensing device
|9 Mar 2006
|3 Aug 2006
|Klein Richard B
|Over-door shoe racks
|9 Dec 1999
|22 May 2007
|Lynk, Inc.
|Hanging shoe rack
|U.S. Classification
|211/35, 211/104, 33/8
|International Classification
|Cooperative Classification
|European Classification


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text:
Page 1
Desalted deep-sea water improves cognitive function
in mice by increasing the production of insulin-like
growth factor-I in the hippocampus
NAOAKI HARADA, JUAN ZHAO, HIROKI KURIHARA, NAOMI NAKAGATA, and KENJI OKAJIMA
function by increasing the hippocampal production of insulin-like growth factor-I
(IGF-I) in mice. In the current study, we examined whether oral administration of de-
salted deep-sea water (DSW) increases the hippocampal production of IGF-I by
stimulating sensory neurons in the GI tract, thereby improving cognitive function in
mice. Desalted DSW increased calcitonin gene-related peptide (CGRP) release
from dorsal root ganglion (DRG) neurons isolated from wild-type (WT) mice by acti-
vating transient receptor potential vanilloid 1. The plasma levels of IGF-I and tissue
levels of CGRP, IGF-I, and IGF-I mRNA in the hippocampus were increased by oral
administration of desalted DSW in WT mice. In these animals, nociceptive information
originating from the GI tract was transmitted to the hippocampus via the spinothala-
micpathway.Improvementofspatiallearningwasobservedin WTmiceafteradmin-
istration of desalted DSW. Distilled DSW showed results similar to those of desalted
DSW in vitro and in vivo. None of the effects of desalted DSW in WT mice were ob-
served after the administration of desalted DSW in CGRP-knockout (CGRP-/-)
mice. No volatile compounds were detected in distilled DSW on GC-MS analysis.
These observations suggest that desalted DSW may increase the hippocampal
IGF-I production via sensory neuron stimulation in the Gl tract, thereby improving
cognitive function in mice. Such effects of desalted DSW might not be dependent
on the minerals but are dependent on the function of the water molecule itself.
(Translational Research 2011;158:106–117)
Abbreviations: BrdU ¼ 5-bromo-2'-deoxyuridine;cDNA ¼ complementary DNA;CGRP¼ calcito-
nin gene-related peptide; CPZ ¼ capsazepine; CT ¼ computed tomography; DG ¼ dentate gy-
rus; DRG ¼ dorsal root ganglion; DSW ¼ deep-sea water; GC-MS ¼ gas chromatography-mass
spectrometry; GFAP ¼ glial fibrillary acidic protein; GH ¼ growth hormone; GI ¼ gastrointestinal;
choleterolemic; LDL ¼ low-density lipoprotein; mRNA ¼ messenger RNA; NTS ¼ solitary tract
potential vanilloid 1; VEGF ¼ vascular endothelial growth factor; WT ¼ wild type
From the Department of Translational Medical Science Research,
Nagoya City University Graduate School of Medical Sciences,
Nagoya, Japan; Department of Physiological Chemistry and
Metabolism, University of Tokyo Graduate School of Medicine,
Tokyo, Japan; Division of Reproductive Engineering, Center for
AnimalResourcesand Development,
Kumamoto, Japan.
Kumamoto University,
Submitted for publication August 30, 2010; revision submitted
February 16, 2011; accepted for publication February 16, 2011.
Reprint requests: Kenji Okajima, MD, PhD, Department of Transla-
tional Medical Science Research, Nagoya City University Graduate
School of Medical Sciences, Kawasumi 1, Mizuho-cho, Mizuho-ku,
Nagoya 467-8601, Japan; e-mail: email@example.com.
1931-5244/$ - see front matter
? 2011 Mosby, Inc. All rights reserved.
Page 2
Deep-sea water (DSW) is water drawn from deep in the
ocean, usually from more than 200 m below the surface.
Water at those depths is free of the contaminants and
pollutants that can taint surface water. Deep-sea water
and minerals such as Mg, Ca, and K.1
The accumulation of lipid and permeation of mac-
for 4 weeks have been shown to be inhibited by the ad-
(LDL) cholesterol levels and cholesterol accumulation
in the arterial wall in rabbits fed 1% cholesterol for 12
weeks were inhibited by administration of desalted
DSW.3Cardiovascular hemodynamics observedin Kur-
osawa and Kusanagi-Hypercholeterolemic (KHC) rab-
bits were improved by drinking of desalted DSW for 6
Although these beneficial effects of desalted DSW
may be related to minerals contained in this water, ther-
apeutic mechanism(s) underlying these beneficial ef-
fects of desalted DSW are not fully understood.4
Insulin-like growth factor-I (IGF-I) is a basic peptide
composed of 70 amino acids with a ubiquitous distribu-
tion in various tissues and cells, which mediates the
growth-promoting actions of growth hormone (GH)
and plays an important role in postnatal and adolescent
Cardiac abnormalities associated with GH deficiency
are corrected by GH therapy, and IGF-I protects against
endothelial dysfunction, atherosclerotic plaque devel-
opment, insulin resistance, and ischemic myocardial
damage.6,7Because IGF-I has vasorelaxant effects8
and blood pressure is increased in mice with severe
IGF-I deficiency,9an increase of IGF-I production
might contribute to the normalization of blood pressure.
Furthermore, IGF-I has been shown to decrease lipid
levels in type 2 diabetic patients.10These observations
suggest that IGF-I may play criticalroles inmaintaining
cardiovascular homeostasis.
Because IGF-I has been shown to increase LDL re-
ceptor expression in HepG2 cells in vitro11and it has
tients,11it is possible that improvement of lipid metab-
olism induced by administration of desalted DSW in
rabbits fed 1% cholesterol might be at least in part ex-
plained by the actions of IGF-I. These observations sug-
gest the possibility that drinking desalted DSW might
increase IGF-I production, thereby improving cardio-
vascular abnormalities and hyperlipidema in KHC
Capsaicin-sensitive sensory neurons are nociceptive
neurons thatcanbefoundinmanytissueswithin thelin-
ing epithelia, around blood vessels, and associated with
nonvascular smooth muscle and myocardium of the
atria.12These sensory neurons release calcitonin gene-
related peptide (CGRP) after activation by a wide
variety of noxious physical and chemical stimuli of
the transient receptor potential vanilloid 1 (TRPV1) ex-
pressed on them,13thereby exerting sensory-efferent
functions. CGRP, which is a 37-amino acid neuropep-
tide, is produced by the alternative splicing of the calci-
tonin gene.14It is widely distributed in the central and
peripheral nervous systems15and has been considered
to possess diverse functions.16We reported that CGRP
increases IGF-I production rapidly via increasing its
transcription in various tissues in mice administered
Capsaicin-sensitive sensory
CGRP are also present in the gastrointestinal (GI) tract,
and these neurons are predominantly spinal in origin.18
Furthermore, nociceptive information originating from
the GI tract is transmitted to the brain via the spino-
parabrachial pathway.19
IGF-I has been shown to enhance excitatory synaptic
transmission in the CA1region of the hippocampus20
and to improve spatial learning by inducing neurogene-
sis in the hippocampus,21,22indicating that IGF-I im-
proves cognitive function by increasing the plasticity
and neurogenesis in the hippocampus. We reported
creased IGF-I production in the hippocampus via in-
creasing in itstranscription,
cognitive function in mice.23
Based on these observations, we hypothesized that
oral administration of desalted DSW might increase
neurons containing
Harada N, et al.
The stimulation of sensory neurons in the gastroin-
testinal (GI) tract improves the cognitive function
by increasing the hippocampal production of
insulin-like growth factor-I (IGF-I) in mice.
Translational Significance
Oral administration of desalted deep-sea water
(DSW) increased tissue levels of IGF-I in the hip-
GI tract, thereby improving the cognitive function
in mice. These observations raised the possibilities
that drinking of desalted DSW might inhibit the
cognitive decline in the elderly and that it might
improve the cognitive function in patients with
the Alzheimer disease.
Translational Research
Volume 158, Number 2Harada et al
Page 3
IGF-I production in the hippocampus through stimula-
tion of sensory neurons in the GI tract, thereby improv-
ing cognitive function in mice. We examined this
hypothesis by analyzing the effect of desalted DSW
on sensory neurons stimulation in vitro and further ex-
amined the effect of oral administration of desalted
DSW on plasma levels of IGF-I, the hippocampal
IGF-I production, and cognitive function in wild-type
(WT) and CGRP knockout (CGRP-/-) mice in vivo. In
addition, we compared effects of desalted DSW with
those of distilled DSW in vitro and in vivo, to examine
on minerals.
Chemicals. Desalted DSW (Muroto deep-sea water)
was provided kindly by Asakawa Natural Food
Company(Kochi, Japan).
capsazepine (CPZ; Sigma-Aldrich, St. Louis, Mo) and
protein kinase A (PKA) inhibitor KT-5720 (Alexis,
Basel, Switzerland) were dissolved in 10% Tween 20/
10% methanol (10%) with normal saline, respectively.
5-bromo-2'-deoxyuridine (BrdU) was purchased from
Sigma Chemical Co. All other reagents were of
analytical grade.
Distillation of desalted DSW. Demineralization of de-
salted DSW was performed by the vacuum distillation
under the condition of 55?C and 20.9 Mpa. Demineral-
ization was confirmed by low electric conductivity of
the water using AOL-40 (DKK-TOA Co., Tokyo,
JAPAN). The levels of major elements in desalted
DSW and distilled DSW are showed in Table I.
analysis. Volatile components in the distilled DSW
and distilled water were estimated by headspace GC-
MS system (HP 5973, Hewlett Packard) equipped
with J&W DB-624 column (0.32 3 60 m). The GC
oven temperature was set at 45?C for 5 min, and then
increased to 180?C with a 5?C/min heating rate. The
temperature was raised further to 220?C with a 10?C/
min heating rate, and then kept at 220?C for 10 min.
GC-MS analysis were realized in the electron impact
Generation ofaCGRPdeficientmice. Thegenerationof
aCGRP deficient (CGRP-/-) mice was described previ-
The mouse computed tomography (CT)/
aCGRP genomic DNA was cloned from a BALB/c
mouse genomic library in EMBL3 using synthetic
oligonucleotide probes derived from the mouse CT/
aCGRP complementary DNA (cDNA) sequence. A
7.0-kb fragment containing exons 3 to 5 of the mouse
CT/aCGRP gene was subcloned into pBluescript
(Stratagene, La Jolla, Calif). A targeting vector was
constructedby replacing
the 1.6-kbXbaI-XbaI
fragment encom- passing exon 5, which is specific for
aCGRP, with the neomycin resistance gene and
flanking the thymidine kinase gene. This plasmid was
linearized with NotI and introduced into 129/Sv-derived
SM-1 embryonic stem cells by electroporation, after
which the cells were selected in medium containing
G418 (300 mg/mL) and ganciclovir (2 mmol/L).
polymerase chain reaction (PCR) and Southern blot
analysis. Targeted embryonic stem cell clones were
injected into C57BL/6 mouse blastocysts to generate
chimeric mice. Male chimeras were then crossbred
with C57BL/6 females and germline transmission
was achieved.Littermates
heterozygotes with the genetic background of the 129/
SvXC57BL/6 hybrid were used for phenotypic analysis.
Only males were used in this study.
Genotype determination of CGRP-/- pups. Genomic
DNAwas extracted from tails of mice as previously de-
scribed24and was used for PCR analysis. PCR was
performed using the external primers of the replaced
gene fragment. The wild-type allele and the mutant
allele gave different band sizes. Primer sequences and
PCR conditions have been described.24
Isolation and culture of dorsal root ganglion (DRG)
neurons. The lumbar, cervical, and thoracic DRG neu-
rons were dissected from both sides of spinal cord of
mice as previously described.25After 5 days in culture,
the medium was aspirated gently and washed with
serum-free Ham's F-12 medium. The cells were
incubated with desalted DSW (20%), distilled DSW
(20%) or media for 30 min in Ham's F-12 medium
containing 1% supplemented calf serum without nerve
growth factor. After incubation, the supernatants were
collected and stored at 220?C for CGRP measurement.
To determine whether desalted DSW increased CGRP
release from DRG neurons via TRPV1 activation, we
examined the effect of CPZ, an inhibitor of TRPV1
activation,26on desalted DSW-induced CGRP release
from DRG neurons. CGRP levels were determined
using specific enzyme immunoassay kit (SPI-BIO,
Massy, France). Recent studies demonstrated that
were identifiedby
obtainedby breeding
Table I. Levels of major elements in desalted deep-
sea water (DSW) and distilled DSW
Levels in water
Desalted DSWDistilled DSW
Na (mg/L)
K (mg/L)
Ca (mg/L)
Mg (mg/L)
Translational Research
August 2011
Harada et al
Page 4
cAMPplays a criticalrole in CGRPrelease fromsensory
neurons by phsphorylating TRPV1 through activation of
and cAMP-dependent PKA activation is
critically involved in CGRP production in DRG
neurons.28Therefore, we examined the effect of
KT5720, an inhibitor of PKA, on desalted DSW-
induced CGRP release from DRG neurons.
AnimalsandDSWadministration. Thecareandhandling
described subsequently approved by the Nagoya City
University Animal Care and Use Committee. Age-
matched (10- to 12-week-old, 21- to 24-g) male
C57BL/6 wild type (Nihon SLC, Hamamatsu, Japan)
and CGRP-/- mice were used in each experiment. They
were maintained under standard conditions of tem-
perature (23–25?C) and on a 12-h light/dark cycle. Food
was provided ad libitum. Desalted DSW and distilled
DSW were given to mice freely for 4 weeks. Control
animals were received distilled water. The animals were
sacrificed at the end of each treatment period. The mice
were anesthetized with intraperitoneally injection of
ketamine (100 mg/kg) and xylazine (10 mg/kg). For
immunohistochemistry sample, mice were injected
intraperitoneallywith BrdU
consecutive days before euthanization.
CGRP. Tissue levels of CGRP were determined in ani-
mals by modification of the methods as described pre-
homogenized in a polytron type homogenizer (2 times
of 15 s) using 0.5 mL of 2N acetic acid. The
homogenates were bathed in 90?C water for 20 min
and then centrifuged at 4500 g for 10 min (4?C).
CGRP was extracted from the supernatant by using
reverse-phase C18columns
Chalfont, UK). Columns were prepared by washing
with 5 mL of methanol, followed by 10 mL of water
before use. The samples were applied onto the column,
followedby washingwith
trifluoroacetic acid. CGRP was eluted with 3 mL of
60% acetonitrile in 0.1% trifluoroacetic acid, and the
solvent was evaporated under a stream of nitrogen gas.
The concentration of CGRP was assayed by using
a specific enzyme immunoassay kit (SPI-BIO). The
antiserum cross-reacts 100% with rodent a-CGRP and
b-CGRP according to the manufacturers data sheet.
The results are expressed as micrograms of CGRP per
gram of tissue.
(50mg/kg) for5
20 mL of 0.1%
levels. Tissue levels of IGF-I were determined in
animals by modification of the methods as described
The hippocampus
homogenized in a polytron type homogenizer (2 times
of plasmaand hippocampal IGF-I
was minced and
of 15 s) using 0.5 mL of 1 N acetic acid according to
the manufacture's instruction. The homogenates was
than centrifuged at 4500 g for 10 min. The supernatants
were kept in a deep freezer at 280?C. Blood samples
were collected in test tubes that contained 10 IU/mL
sodium heparin as according to the manufacturer's
instructions. Blood was centrifuged at 2000 g for 10
min (4?C). The concentration of IGF-I was assayed by
using a mouse/rat IGF-I EIA kit (Diagnostic Systems
Laboratories Inc., Webster, TX).
Quantitative messenger RNA (mRNA) analysis. Quanti-
ously.31The tissue was weighed and immersed in liquid
nitrogen.Total RNAwas
hippocampus with TRIZOL Reagent (Invitrogen,
Carlsbad, Calif) according to the manufacturer's
instructions. RNA extracted was used as a template for
cDNA reverse transcription. Sample cDNAs were
amplified in the Model 7700 Sequence detector
(Applied Biosystems, Perkin Elmer Japan, Chiba,
Japan) with primers, dual-labeled fluorogenic probes,
and a Taqman PCR Reagent Kit (Applied Bio-
systems). Thermal cycler conditions were 10 min at
95?C for deactivation preceding 40 cycles for 15 s at
95?C for denaturation and 1 min at 60?C for bath
annealing and extension. Known concentrations of
serially diluted IGF-I and b-actin cDNA generated by
PCR were used as standards for quantitation of sample
cDNA. The copy numbers of cDNA for IGF-I were
standardized those for b-actin from same sample.
Immunohistochemistry. For histochemical analysis,
the mice were anesthetized and perfused with 0.01
mol/L phosphate-buffered saline (PBS) followed by
4% paraformaldehyde in 0.1 mol/L PBS (pH 7.4), and
then stored in PBS with 20% sucrose for 24 h. The
isolated brains were frozen in an OCT compound
(Tissue Tec; Miles, Elkhart, IN) and serial sections
at 20 mmol/L thickness were used for immuno-
fluorescenceas described.32
staining were treated for DNA denaturation with 2N
HCl. Sections were incubated with primary antibodies
at the following concentrations: rabbit anti-c-fos
polyclonal antibody (1:500; Santz Cruz Biotechnology
Inc., Santa Cruz, Calif), mouse antiglial fiblillary acidic
protein (GFAP) polyclonal antibody (1:1000; Dako,
Glostrup, Denmark), mouse anti-BrdU monoclonal
antibody (1:100; Invitrogen), rat anti-CD31 mono-
clonal antibody (1:200, BD Biosciences PharMingen,
San Diego, Calif), and mouse anti-calbindin D28k
monoclonal antibody (1:200; Abcam, Cambridge,
UK). After incubating at 4?C overnight and washing,
the sections were treated with secondary antibodies,
Alexa Fluor 568 anti-rabbit immunoglobulin G (IgG)
for c-fos, Alexa Fluor 488 anti-mouse IgG for BrdU
extracted fromthe
Translational Research
Volume 158, Number 2Harada et al
Page 5
and calbindin D28k, Alexa Fluor 568 anti-rabbit IgG
for GFAP, and Alexa Fluor 594 for anti-rat IgG for
CD31 (1:500; Invitrogen) for 1 h at room temperature.
Negative controls were performed by omitting the
primary antibodiesduring
Samples were then mounted and photographed under
light fluorescence microscope (Axio Image A1; Carl
Zeiss, Aagen, Germany).
the immunostaining.
The BrdU-positive cells in the subgranular, granule
cell layer, and in the hilus of the hippocampus was
counted using the stereologic counting method. Five
sections from the hippocampus were cut beginning
1.46 mm caudal and 1.86 mm anterior to the bregma
with intervals of 100 mm. To assess the phonotype of
BrdU-positive cell in double immunofluorescence,
a mean value for each marker was obtained from 5
sections from 5 mice. The number of c-fos–labeled
cells was quantified in the dorsal horn (lamina I-II)
of spinal cord, parabrachial nuclei (PBN), the solitary
tract nucleus (NTS), and the hilus of the hippocampus.
The number of cells from NTS, PBN, and hilus was
counted from three sections of each brain (n 5 5 for
each group), corresponding to planes 6.64, 4.64, and
1.86 mm anterior to bregma. Because of the spread lo-
calization of GFAP, we used fluorescence intensity to
quantify the average intensity of each field by ImageJ
software, and the data were expressed as percent
Morris water maze task. Behavioral testing was con-
ducted as described previously.33We used a circular
pool (120-cm diameter), with hidden targets on the
walls. The pool was filled with water at 30?C and
contained a round-shaped transparent acrylic platform
(12-cm diameter). In the task, the platform was
submerged 1 cm below the surface of the water and
located in the southeast quadrant of the pool
throughout the trials. After the mouse was put into the
pool, each had a maximum of 90 s to locate and climb
onto the platform (1 trial). When a mouse located the
platform, it was allowed to stay on it for 20 s. The
mice that did not find the platform in the allowed time
were placed on it by the experimenter and left there
for 20 s. Latency to reach the platform was monitored.
Each mouse was subjected to 1 trial per day. The task
consisted of 5 days of trials. Animals were trained in
the Morris water maze for 1 trial per day for 5 days.
Reference memory test (probe trial) was performed in
the absence of the platform 2 h after the last trial.
During the reference memory test, the trial was
performed with the cutoff time of 90 s. The latency to
reach the platform, swim speed, and for the reference
memory tests, the time spent in the target area (zone
radius: 30 cm, 3 times the target diameter), were
recorded with a digital camera system.
Statistical analysis. Data are expressed as the mean 6
standard deviation (SD). The results were compared us-
ing an analysis of variance followed by Scheff? e's post
Effects of desalted DSW and distilled DSW on CGRP
releaseinDRGneuronsisolatedfromWTmice. Toexamine
whether desalted DSW stimulates sensory neurons, we
examined the effect of desalted DSWon CGRP release
from DRG neurons in vitro. Desalted DSW increased
CGRP release from DRG neurons isolated from WT
mice (Fig 1). Distilled DSW also increased CGRP
release from DRG neurons, while distilled water did
not (Fig 1). Pretreatment with the selective transient
receptor potential vanilloid 1 (TRPV1) inhibitor CPZ
reversed this effect of desalted DSW in DRG neurons
(Fig 1). Cyclic adenosine monophosphate (cAMP) has
been shown to play an important role in the release of
CGRP from the sensory neurons after activation.27
TRPV1 is activated by phosphorylation through
desalted DSW might increase CGRP release from
sensory neurons by sensitizing TRPV1 activation
Fig 1. The effects of desalted DSW and distilled DSW on CGRP re-
lease in culturedDRG neuronsisolated from WT mice, and the effects
ofCPZandKT5720ona desaltedDSW-inducedincreaseinCGRPre-
lease from DRG neurons isolated from WT mice. DRG neurons were
incubated with desalted DSW (20%), or distilled DSW (20%) for 30
min. Furthermore, DRG neurons were incubated with desalted DSW
(20%) in the presence of the inhibitor of TRPV1 activation CPZ (10
mmol/L) or the PKA inhibitor KT5720 (10 mmol/L). Supernatants
were collected and CGRP levels were measured by enzyme immuno-
assay. Values are expressed as means 6 SD from 5 experiments. *P ,
0.01 vs media.†P , 0.01 vs desalted DSW.
Translational Research
August 2011
Harada et al


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1. Gilles Proulx : portrait d'un tirailleur tiraillé
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The governments of Thailand and Britain have deemed the GT200 bomb detector unreliable, but the Thai military continues to use it to make arrests in the Muslim south.
In a blow to Thailand's former prime minister, Thaksin Shinawatra, the Supreme Court seized $1.4 billion of his fortune. His allies have vowed ongoing street protests.
Thailand has deployed thousands of security forces ahead of a court verdict Friday on $2.2 billion in frozen assets belonging to an exiled but still-popular former Prime Minister Thaksin Shinawatra.
In Beijing, sending a child to kindergarten costs as much as $660 a month, compared with $102 a month for the country's top college.
Philippines troops killed Abu Sayyaf commander Albader Parad on Sunday, dealing a major blow to the Islamic militant group.
Islamic police in Aceh, Indonesia, patrol daily for women wearing tight clothes and unmarried couples sitting too close.
Riling China, the US's newest $6.4 billion sale includes 60 Blackhawk helicopters, Patriot missiles, and sophisticated command-and-control software.
Wealthy Indonesian convicts can purchase prison cell upgrades, an investigation at a women's prison in Indonesia found last month. Amenities range from air conditioning to maid service.
Malaysia caned three women after they were convicted for adultery under Islamic law, the first time that punishment has been handed out inside the country.
Choi Hyun-mi, who fled to South Korea as a girl and soon won a world boxing championship, has helped boost the morale of fellow North Korean defectors who continue to struggle in their adopted country.
In Japan, Toyota recall woes were met first with disbelief and then with an onslaught of criticism from Japanese media outlets more accustomed to eulogizing the 'Toyota way.'
On Kim Jong-il's birthday, the North Korean leader's attention appeared focused on grooming his youngest son, Kim Jong-un, for succession. The senior Kim did not attend his birthday bash.
Two Greenpeace activists in Japan face up to 10 years in prison for tactics used in exposing black market sales of whale meat. Anti-whaling groups hope Monday's trial helps turn Japanese public opinion against the whale harvest.
UN undersecretary-general Lynn Pascoe said today that North Korea is 'not eager to return to six-party talks,' but others say Kim Jong-il appears ready for renewed dialogue.
China was criticized by the US ambassador and other foreign diplomats after a Beijing court rejected the appeal of top Chinese dissident Liu Xiaobo against his 11-year jail sentence, the severest in recent memory.
Campaigning for the Philippines elections kicked off this week in familiar style, with famous names dominating the ballots and already 60 people killed in political violence. Here's what to look for ahead of the May polls.
Tan Zuoren, who had investigated school collapses that killed thousands of children in China's massive 2008 earthquake, was sentenced to five years.
North Korea has said it will release US missionary Robert Park, who crossed into North Korea on Christmas Eve bearing a message of 'peace and goodwill' for Kim Jong-il. North Korean officials said the young American had retracted his views of the North as a repressive place.
North Korea fired its chief financial planner in wake of a currency revaluation that sparked public anger, according to reports in South Korean media. One outlet reported that crowds were besieging marketplaces as prices rise.
Gao Zhisheng, once praised by the Chinese government as a star lawyer, remains missing one year after police dragged him from his home. Rights groups are particularly worried about the treatment of the human rights lawyer.
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Lawyers should pay attention to a Bankruptcy Court judge's decision that may not have made big headlines, but could impact a very critical area of lawyering; namely, delegating duties to a paralegal — particularly those not in the full-time employ ...Read More »
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A CJAM Who Weathered The Storm
As Chief Justice for Administration and Management Barbara A. Dortch-Okara steps down this month after a five-year tenure, the best way to describe her performance may be "grace under fire." The CJAM, given the gargantuan task of righting a court ...Read More »
In Search Of Judicial Accountability
In enacting the last major overhaul of the courts, the Legislature required the judiciary to devise and implement a system of judicial evaluations. The courts were slow to respond, finally conducting systemic internal judicial evaluations after the Massachusetts Bar Association ...Read More »
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There is growing confusion over the Massachusetts Homestead Law and it is creating a frustrating situation for lawyers. According to some attorneys, the law is unclear whether new homestead declarations need to be filed in the case of both refinances ...Read More »
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The Boston Bar Association voted recently to adopt the recommendations of its Judicial Response Task Force, which will set criteria and procedures for bar association responses to public attacks on judges. Citing a number of occasions where judges have not ...Read More »
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Posted November 24, 2012 on AP on Fox
Doug McDermott and his dad felt differently about the younger McDermott's performance Friday night. His dad's opinion, though, carries more weight. Greg McDermott is No. 14 Creighton's coach. Doug had 30 points and eight rebounds to lead Creighton past Wisconsin 84-74 for a spot in the Las Vegas Invitational final. "I got off to a slow start (shooting). I missed a lot of shots I normally make," Doug McDermott said. "I need to get to the post. I thought I did a great job in the post. I felt really comfortable being patient and making reads out of the post." Creighton (5-0) will play Arizona State in the championship game Saturday. The Sun Devils beat Arkansas 83-68. "We let Doug go to work," Greg McDermott said. "He made really good decisions. He took what the defense gave him. His pace was better offensively tonight." Ethan Wragge had 17 points, including a 3-pointer that put Creighton up for good, 61-60 with 7:40 left at the Orleans Arena. Austin Chatman added a career high ...
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text: Good introductions to phylogeny are included in E.O. Wiley and Bruce S. Lieberman, Phylogenetics: The Theory and Practice of Phylogenetic Systematics, 2nd ed. (2011); Mark Ridley, Evolution and Classification: The Reform of Cladism (1989); David L. Hull, Science as a Process: An Evolutionary Account of the Social and Conceptual Development of Science (1998); Peter L. Forey et al., Cladistics: A Practical Course in Systematics (1992; reprinted 2002); Paul H. Harvey et al. (eds.), New Uses for New Phylogenies (1996); Joseph Felsenstein, Inferring Phylogenies (2004); Richard Cowen, History of Life, 5th ed. (2013); and Andrew Purvis, John L. Gittleman, and Thomas Brooks (eds.), Phylogeny and Conservation (2005).


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The Beverly Hills Hotel Relaunches Polo Lounge
Tuesday April 23rd, 2013 - 10:31AM
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LOS ANGELES—The 73-year-old Polo Lounge at Dorchester Collection's Beverly Hills Hotel has been reintroduced after a two-month restoration program. Led by designer Adam Tihany, the subtle refresh to the Polo Lounge pays homage to its heritage and iconic design elements.
"The Beverly Hills is a dream project. I love this hotel. And it is so important that it continues to respect the spirit of the city," Tihany said in a statement. "By maintaining much of the Polo Lounge's beloved originality, we have not only kept the connection to the community but also enhanced it."
The room continues to celebrate California's relaxed lifestyle with its green and white stripes and similar color scheme still intact. Updated carpets and upholsteries reflect the hotel's warm residential feel, and a new hostess stand has been added. Fixtures, lighting and furniture were replaced, and ornate mirrors, historic images and renewed rich, dark paneling adorn the walls. In the patio's outdoor space, enhanced heating, sound systems and improved lighting have been added. Flower settings, vintage lamps, Riedel glassware, and new linens add the finishing touches.
Executive Chef Kaleo Adams' menu continues to offer traditional American fare. Changes to presentations and service delivery have enhanced the dining experience. Afternoon tea, complete with a tea expert has been reintroduced on Friday and Saturday. Wine lists are also now offered on iPads, creating an interactive experience that enables guests to visually select their wine of choice.
"The Polo Lounge is lovingly referred to as 'Hollywood's commissary' and will forever be a timeless fixture in the community," according to Ed Mady, regional director, West Coast, USA and general manager. "We felt a tremendous responsibility to maintain much of its wonderful originality while also providing an enhanced and relevant dining experience to our countless regular guests and first-time visitors. The Polo Lounge is and always will be a very special place for generations of very special people."
In addition to the new lobby and refreshed Polo Lounge, the hotel's restoration program continues at the pool cabanas and Cabana Café, followed with all guestrooms and suites being updated by the summer of 2014.
Tags: • Hospitality • Construction/Renovation • FF+E/Purchasing •
From lifestyle choices to luxury decisions. Back in January, when we unveiled the newly redesigned Hotel Business, Eric Danziger was the subject of our cover story for the ALIS issue. He had recently joined Hampshire Hotels Management as president and CEO, and had just transformed the operator into a full-fledged brand company, Début Hotel Group, which would become the umbrella for five brands (Augustus Hotels & Resorts, Dream Hotels, Time Hotels, Unscripted Hotels and Night Hotels).
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Plaintiffs bring class action claims for damages and injunctive relief against motor fuel retailers in Alabama, Arizona, Arkansas, California, Delaware, Florida, Georgia, Indiana, Kansas, Kentucky, Louisiana, Maryland, Mississippi, Missouri, Nevada, New Jersey, New Mexico, North Carolina, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Utah, Virginia, the District of Columbia and Guam. Plaintiffs claim that because defendants sell motor fuel for a specified price per gallon without disclosing or adjusting for temperature expansion, they are liable under state law theories which include breach of contract, breach of warranty, fraud and consumer protection. Following a transfer order of the Judicial Panel on Multidistrict Litigation ("JPML"), the Court has jurisdiction over consolidated pretrial proceedings in these actions.
All claims against all defendants have either been (1) settled, (2) resolved on the merits, (3) dismissed or (3) transferred back to the transferor court(s).
On April 24, 2012, the Court certified a settlement class and granted final approval to the amended settlement with Costco Wholesale Corporation. See Memorandum And Order (Doc. #4248).
On August 21, 2015, the Court certified settlement classes and granted final approval to 28 settlements. See Memorandum And Order (Doc. #4851).
Class counsel's motions for attorney's fees, expenses and class representative incentive awards remain pending. On or before October 2, 2015, class members may file objections to the fee requests. On November 19, 2015, at 9:30 a.m. in Courtroom 476, the Court will hold a hearing regarding the fee requests.
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Husch Blackwell LLP
4801 Main Street - Ste. 1000
Kansas City, MO 64112
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All hotels in Groningen, Netherlands
1 room, 2 adults
Kleine Kromme Elleboog 7-b, Groningen, 9712 BS, Netherlands, 1-877-214-4288
University Hotel - Groningen
Lowest price$75
3-star hotel with bar/lounge, near Grote Markt
- Free WiFi
Main amenities
- 56 smoke-free guestrooms
- Breakfast available
- Bar/lounge
- Coffee/tea in the lobby
- Meeting room
- Free newspapers in the lobby
- Tour/ticket assistance
What's around
- Heart of Groningen
- Grote Markt (0.3 mi / 0.4 km)
- Groninger Museum (0.5 mi / 0.8 km)
- University of Groningen (0.1 mi / 0.1 km)
Just a 10-minute walk from University of Groningen, Grote Markt and Groninger Museum, University Hotel offers 3-star Groningen city center hotel accommodations. You'll also be within 5 minutes of Universiteitsmuseum and Vismarkt.
This hotel offers free WiFi and wired Internet access. Guests wishing to enjoy a drink will surely want to visit the bar/lounge. Staff can provide tour/ticket assistance.
This hotel offers free WiFi and wired Internet access. Guests wishing to enjoy a drink will surely want to visit the bar/lounge. Staff can provide tour/ticket assistance.
I got no sleep thanks to the fact that the hotel shares a courtyard with a pub. The…
The personal service at the University Hotel were excellent and the personnel quite…
TripAdvisor Traveller Rating: 3.5
From 46 reviews
Enter dates to check prices
Hotel Info: 1-877-214-4288from $75
- Standard Room, 1 Single Bed
- Standard Double Room
- Standard Twin Room
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 56 rooms
- 24-hour check-in
- Check-out time is 11 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- No cribs (infant beds)
- Pets not allowed
- Free WiFi and wired Internet access in public areas
- Free WiFi and wired Internet access in rooms
Other information
- Smoke-free property
In the hotel
Food and drink
- Breakfast (surcharge)
- Bar/lounge
- Coffee/tea in the lobby
Working away
- Conference space size (feet) - 323
- Conference space size (meters) - 30
- One meeting room
- Tours/ticket assistance
- Free newspapers in lobby
In the room
Sleep well
- Hypo-allergenic bedding available
Freshen up
- Private bathroom
- Shower only
- Hair dryer (on request)
Be entertained
- Flat-screen TV
Stay connected
- Desk
- Free WiFi
- Phone
University Hotel - Groningen's small print
Special cancellation policies or charges may apply for group reservations (more than 8 rooms for the same hotel / stay dates).
Mandatory fees
You'll be asked to pay the following charges at check in or check out:
- A tax is imposed by the city: EUR 3.00 per person, per night
Optional extras
Rollaway beds are available for EUR 15 per night
Breakfast is offered for an extra charge of EUR 11.50 per person (approximately)
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


text:
Quintana Roo - No Man's Land
Live Webcam Image from Cozumel - Punta Langosta
This is a
webcam view from our downtown oceanfront office
next to Punta Langosta Cruise Ship Pier.
This picture was taken 12:11AM (EST) Friday, Oct 9
A new photo is posted every 1 minute all day 24 hours a day! The camera is currently
Take a LOOK at INSIDER'S PalMar SMALL Beach Club webcam #2!
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ATTENTION! GEO-CACHE Treasure Hunters ~
GPS for WEBCAM 1
We now have 2 office locations to better serve you!
602-B Melgar (Upstairs)
Between Navy Base & Hotel Barracuda
(50 yards SOUTH of Punta Langosta Pier)
IslaMar Vacation Villas
rated "excellent" by many travelers
Phone: 987-869-0504
316 / 332 55 Avenida
Between Calle 3Sur & Morelos
(15 minute walk into town area)
Phone: 987-869-0504
Depending on season, one office or the other could be closed part of the day. So call first to see where we are ~ we would love to meet you!
But please do
our office, say "HI" and pick up a
FREE COZUMELINSDER MAP, PEN or KEYCHAIN!
We'd like to meet you and assist you with excursions, rental cars, and island accommodations!
A Personal Note from Sherri "A Beautiful Summer!"
Well our Spring Cleaning efforts were huge this year and yielded several very successful garage sales at IslaMar Villas. All the drama of a few years back put me very far behind on getting rid of stuff in my closets and bodegas and staying organized in my office. But thanks to all the wonderful, enthusiastic staff on board with us now, I was able to dedicate a lot of time and focus to cleaning and purging. Im not at all finished but at least I got a first pass done in all categories of purging so as to create some elbow room in the storage areas so we can get better organized for the season to come.
Its been a beautiful summer on the island with very little rain thus far in the season but were just now headed into the peak of the rainy months so Im sure well have our share of precipitation here soon. As school is about to get underway again, its a great time to consider a quick weekend getaway to the island! Think about it and look for airfare specials or cruise line discounts because being here on the island during low season means far fewer people roaming around and at times ~ it actually seems dead and deserted here! So come on down and help liven things up for us as we await the arrival of high season here in just a few months! Well be watching for you!
Check Out Sherri's Previous Writings!
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Cozumel Carnaval 2015 is Here! (2/13/15)
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Time to Celebrate! (12/20/11)
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Pandamonium! (2/13/10)
Mexican Currency (11/22/09)
Taxi Mafia Mathematics (3/3/09)
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PalMar SMALL Snorkel Beach Club
Personal Experiences
Submit Feedback
Sherri, I did receive both email confirmations. Thank you again for your great customer support and service in getting these activities scheduled for me. I will recommend your site to all of my friends who visit the island. Thanks again!!
A. Washburn ~ Not Given
I just wanted to let you know that the private charter van service was outstanding. Thanks so much to you and your staff for arranging for the beverages on board and the driver was great. We will be visiting Cozumel again will always be utilizing yours and Uniper's assistance.
R. Lee Family ~ Colorado
I must admit, booking our excursion through you and not the cruise ship was unsettling but once we got to your facility all of our doubts were gone. Your park and staff were first class, professional, friendly and even the dophins looked happy. Thank you for a truly once in a life time experience. Your quick response to my e-mails was also appreciated. I can assure you I will recommend your park to anyone that tells me they are going on a cruise. Thanks again.
Andressen Family ~ Belle Chasse, LA USA
Thank you so much!!! Thank you for arranging the dolphin encounter for my family!!! Even in the aftermath of Hurricane Emily, Cozumel Insider came through with flying colors --- yall are perfect...
A. Sikes ~ Frankston, TX USA
Thanks Sherri, we like to do business with people who care about what they are doing and not just out for money!
R. McGough ~ U.S.A.
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