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Dr. Gregory Johnston's Ratings
We have had the privilege of having Dr J as our family doctor for over 30yrs and I have to say I am going to miss him...he has be an amazing doctor. He is not only a doctor, he is a wonderful friend. You deserve this retirement....the best to you and Sharon!!! Enjoy:)
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Sad to see him retire, but considering he delivered me 46 years ago, the writing was on the wall. I don't think Simcoe will ever see another doctor of his caliber.
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I've felt for the last two years that this day was coming for Dr. Johnston to retire, but to actually have it happening is quite sad for me, he's been my doctor for the last 30-31 years, he's seen me through my very low points (through my depression in high school and the passing of my brother 4 years ago), and 5 years ago with his help he discovered my meningioma brain tumour. I told him about my painful headaches and kaleidoscope of colours in my vision and his quick actions by sending me to the hospital for a CAT scan found the tumour, he then sent me to a Neurologist the very same week, the following week I had my 13 hour surgery and because of Dr. J that is why I am still here today. He's also looked after my brother, my dad and also my grandmother. I am ever so grateful for the caring & professionalism that Dr J. has always shown to me and that's why its going to be so hard to depend on another doctor the way I have with Dr. J., I will have to learn, but there won't be anyone else like you Dr. Johnston. I wish Dr. Johnston and his wife a great and enjoyable retirement. May it be filled with much happiness, travel, and great health. Take care Dr. Johnston I will miss you. From Mary Ann Gegeny
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Very sad to see Dr. J. retire - but very happy for him at the same time. I have been his patient for almost his entire career - he & Dr. Kursell bought their practice from Dr. Jenkins and were beside the Carlon Tower until moving into the Cedar St. location. He is among the best ever!!! You will be greatly missed but wishing you the best forever!!
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best doctor around happy retirement you deserve it
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He's been my Dr. For 15 years. Wonderful man and hate to see him retire, but he deserves it. Happy retirement!
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A sad day has come! You will be missed Dr. J. Enjoy your retirement!
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Best dr!! Never rushes , helpful, caring and gets to know his patients! Sad day when he retires
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I know Dr. Johnston deserves to retire, but the thought of trusting another doctor at this stage of my life seems extremely difficult. He has seen me through the death of my husband, and has been so caring. He has been our family doctor for at least 45 years. He delivered my children, and was involved with the birth of two of my grandchildren. God Bless You, Dr. Johnston. I will miss you. W. Hildebrand.
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He delivered me, my daughter & would have delivered my son if I didn't have complications with him. Everyone's comments on here about Dr. Johnston are spot on! My next doctor has big shoes to fill.
We will all miss you! Enjoy your retirement, you deserve it!
From the Schram & Spain family
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Steve McGarrett Quotes
Steve: Sometimes when you speak, when your mouth opens and you speak, all I hear is "wa-wa-wa-wa." It's like I'm in a Charlie Brown cartoon only you're Linus, with better hair.
Danny: Why do I got to be Linus?
While they were getting Detective Williams out of jail in Columbia, I was the only one helping you.Gabriel
Chin: That was close.
Steve: Speaking of close, Kono you are getting married in five minutes.
But nobody is gonna want to eat fish for the next three months.Kamekona
You got two kids now and one of them needs you to go home and do something really important for him. So you don't get to die today, even though it would make my life a lot quieter.
Steve: Wow.
Catherine: So you approve?
Danny: Yeah, no I think what my ape like friend meant to say was that you look fantastic.
Steve: Hey listen if things are weird with Rachel let me go get Grace. Its not a problem.
Danny: No, no nothing is weird its fine.
Steve: You are lying.
Steve: Relax. It is residual radiation. You will be fine.
Danny: Oh, thank you Dr. Strangelove
Which one of you has the lowest standards, because I am here.Nolan
Steve: Promise me one thing buddy.
Danny: What?
Steve:Gracie will get her college degree online.
Steve: Why would you even say her name here?
Grover: Can I get a strawberry daiquiri?
Steve: Did you say strawberry daiquiri?
Grover: I am comfortable in my manhood brother.
Steve: Oh yes you are. Deep dish pizzas, strawberry daiquiris. You like the finer things in life.
Steve: I am sorry did you just say you just flew 10 hours straight with a bunch of pizzas in your suite case? Is that what you said?
Grover: You're damn right. And today is your lucky day. Because of this unexpected, pleasant little pick me up at the airport I will cut you in on a slice.

subset: commoncrawl


text:
Turlock High wrestling improved to 3-0 in the Central California Conference this week with dual wins over Merced High in Tuesday and Buhach Colony High on Wednesday. The Bulldogs defeated the Bears by winning 10 of the night's 14 matches and put down the Thunder by winning 12 of 15 matches.
Brandon Harris is a Pitman High original.
Pitman High opened Tuesday's game against rival Turlock High with a Britney Leon trey- an early sign of things to come.
Denair High's girls led Southern Athletic League rival Delhi High for the entire first half of Thursday's basketball game, but with one quarter left to play the situation seemed dire for the Coyotes. After struggling to contain Denair's offense, the Hawks closed the gap on the Coyotes and pulled away with a 13 point lead.
ESCALON - A 15-point Hilmar High deficit at the half suddenly expanded in the midst of a 21-8 third quarter run for Escalon's varsity boys basketball team as abysmal scoring plagued the Yellowjackets throughout a 56-33 Trans Valley League defeat on Tuesday.
It was more than just another basketball game Monday night when Turlock Christian High hosted its arch rival Ripon Christian High. The less than spacious Calvary Baptist Gym was packed to the gills as both Eagles (14-3 overall, 4-0 SAL) and Knights (9-6 overall, 3-1 SAL) supporters watched from the bleachers, hallways, and even the floor as the defending Southern Athletic League Co-Champions had it out.
While one former Turlock quarterback wowed the nation in San Francisco on Saturday, another was on the other side of the country doing his best to put in an impressive performance of his own and turn the heads of some college coaches.
Hilmar High notched its first Trans Valley League win Thursday night with a 62-57 victory over Riverbank High at home. The Lady Yellowjackets (13-3 overall, 1-2 TVL) struggled early with inaccurate shooting and a lack of offensive rebounds, but by the end of the first quarter they had beat the Bruins (11-5 overall, 1-2 TVL) back and taken the game's momentum with fast break layups and a peppering of perimeter shots.
Turlock High won its first Central California Conference dual meet of the season on Wednesday when Atwater High came into town and dropped all but one of the night's 14 matches. The Bulldogs (7-3 overall, 1-0 CCC) finished with 68 team points to Atwater's 3, a number boosted by seven pins, two technical falls, and a major decision.
In the recent past, the Central California Conference has been led by Turlock and Golden Valley High wrestling with Pitman High not too far behind. The Pride entered this season determined to re-shuffle those standings and on Wednesday, when the Cougars visited Pitman's gymnasium, they got their chance to topple last season's second place team.
Turlock High snapped a three game losing streak on Tuesday with a victory over Golden Valley High in the Central California Conference opener. The Bulldogs (1-0 CCC) trailed for most of the first half, but a second half comeback effort made it 49-45 by the end of regulation.
A matchup of champions took place Tuesday night between defending Central California Conference co-champs Pitman and Buhach Colony High. The Thunder (1-0 CCC), equipped with five returning starters from last season's team, were favored as they entered Pitman's gym but the Pride (0-1 CCC) quickly doused their confidence with a healthy dose of defense and inside shots.
Turlock High's December schedule was brutal. Over the span of three weeks, Bulldog wrestlers competed in the Clovis West Shootout, the Reno Tournament of Champions, and the Zinkin Classic; gaining valuable experience against top level competition.
Turlock and Laguna Creek High met on the court of Bulldog Arena for the second time this season Friday night. The last time the Bulldogs (9-7) and Cardinals (9-7) faced off the Godinez Championship was on the line and Turlock emerged victorious with an overwhelming 54-36 victory.
Hilmar High put up its most lopsided win of the season against Denair High on Thursday. After recovering from a four game losing streak with a victory over Turlock Christian High a week ago, the Yellowjackets continued their march forward with a 54-20 effort.
Denair High hosted the Mariposa County Grizzlies on Friday night and was unable to come away with a victory after Mariposa's strong first half.
Pitman (3-2) senior running back Blake McBay rushed for 172 yards on 22 carries and a score and the Pride steamrolled through North Salinas High ...
After a scoreless first half at home on Tuesday, head coach Louie Bettencourt strung together some moving words for his Hilmar High varsity boys soccer ...
Both Turlock and Pitman tennis teams were 4-0 before Tuesday's league showdown, but only one team left undefeated in the Central California Conference matchup.
Head coach James Peterson and his Turlock Bulldogs felt like it was their time on Friday when they traveled to Tracy High to take on ...
Last year's matchup between the Denair and Delhi varsity football teams came down to overtime, but this year's game came down to an ...
Hilmar High's varsity football team dominated the visiting Pacific Grove squad on Friday night in their homecoming game.
This week marked the halfway point of the Central California Conference girls golf season and Turlock and Pitman High both finished strong in the Mid-Season ...
Page 1 of 1
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Page 1
Linkage-specific avidity defines the lysine 63-linked polyubiquitin
binding preference of Rap80
Joshua J. Sims1 and Robert E. Cohen1,*
1Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins
University, Baltimore, MD 21205, USA
Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional
outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been
largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-
polyUb) in isolation. Here we propose a mechanism, linkage-specific avidity, in which multiple
ubiquitin binding domains are arranged in space so that simultaneous, high-affinity interactions are
optimum with one polyUb linkage, but unfavorable or impossible with other polyUb topologies and
monoUb. Our model is human Rap80, which contains tandem ubiquitin interacting motifs (UIMs)
that bind to K63-polyUb at DNA double-strand breaks. We show how the sequence between the
Rap80 UIMs positions the domains for efficient, avid binding across a single K63 linkage, thus
defining selectivity. We also demonstrate K48-specific avidity in a different protein, ataxin-3. Using
tandem UIMs, we establish the general principles governing polyUb linkage selectivity and affinity
in multivalent ubiquitin receptors.
The covalent attachment of the small protein ubiquitin (Ub) to other proteins is an essential
step in an enormous variety of cellular processes (Pickart and Eddins, 2004). Substrates can
be modified with a single Ub unit or polymeric Ub chains assembled by the linkage of one Ub
C-terminus to any of seven lysines on another Ub molecule (Peng et al., 2003). Whereas
ubiquitination with chains linked though lysine 48 (K48-polyUb) leads to degradation of the
substrate protein at the 26S proteasome (Pickart and Cohen, 2004), lysine 63-linked polyUb
and monoUb function as distinct but non-proteolytic signaling elements (Sun and Chen,
2004) in pathways such as endocytosis and DNA repair. The prevailing model holds that this
functional diversity is possible because downstream receptors can distinguish the Ub forms by
selective binding (Pickart and Fushman, 2004).
We suspected that some multivalent Ub binding proteins may achieve linkage selectivity by
exploiting the distinct orientation and spacing of Ub units that result from a particular polyUb
linkage. Multiple ubiquitin binding domains (UBDs) could be arrayed in space to optimize
simultaneous interactions with both Ub units in a configuration characteristic of one type of
*Correspondence: E-mail: firstname.lastname@example.com.
Editorial correspondence to: Robert E. Cohen, Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg
School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205-2103, Tel: (410)502-0681, Fax: (410)955-2926, E-mail:
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers
we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting
proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could
affect the content, and all legal disclaimers that apply to the journal pertain.
NIH Public Access
Author Manuscript
Mol Cell. Author manuscript; available in PMC 2010 March 27.
Published in final edited form as:
Mol Cell. 2009 March 27; 33(6): 775–783. doi:10.1016/j.molcel.2009.02.011.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 2
Ub-Ub linkage, but not another. For the target polyUb linkage, binding by the first of multiple
UBDs to one Ub would position the second UBD favorably for interaction with a nearby Ub
in the chain. Because the second binding event occurs between binding partners at high local
concentrations, it is potentially much more favorable. This could form the basis of linkage-
specific polyUb recognition, as well as polyUb vs monoUb specificity. This type of cooperative
binding is termed avidity; hence, we call this mechanism 'linkage-specific avidity'.
We examined the human protein Rap80 as a potential model of linkage-specific avidity. At the
site of DNA double-strand breaks in human cells, an early signaling cascade leads to the
recruitment of the Ub E2 enzyme Ubc13 and the E3 RNF8 which then ubiquitinate one or more
substrates at the site of the damage with K63-linked polyUb (Bennett and Harper, 2008; Kolaset
et al., 2007; Mailand et al., 2007; Wang and Elledge, 2007; Huen et al., 2007). Rap80 links
these early events to proteins critical for repair by binding to the K63-polyUb signal with N-
terminal tandem UIMs (tUIMs), while a central Rap80 domain binds an associated complex
that includes Abraxas, the tumor suppressor BRCA1, and the deubiquitinating enzyme
BRCC36 (Kim et al., 2007; Sobhian et al., 2007; Wang et al., 2007; Wang and Elledge,
2007; Yan et al., 2007). The importance of the localization activity is evident from clinical
BRCA1 mutants that do not assemble into the complex with Rap80 and thus fail to reach the
sites of DNA damage (Sobhian et al., 2007).
Although UIM domains are not known to have a large linkage preference in isolation, Rap80
bound more K63-polyUb than K48-polyUb in GST pull-down experiments (Kim et al, 2007;
Sobhian et al., 2007). UIMs are 18 - 21 amino acids in length and are ideal for structure
prediction and molecular modeling because of their simple, α-helical structure (Fisher et al.,
2003; Hofmann and Falquet, 2001). We performed modeling based on the structure of a UIM
bound to monoUb and found that the 7-residue linker between the Rap80 tUIMs could position
the domains ideally for simultaneous interactions with two K63-linked Ub units. In contrast,
modeling based on a K48-diUb structure predicted that avid interaction with K48-diUb would
be impossible or inefficient, requiring longer K48 chains for simultaneous contacts. Here we
show that, consistent with these models, the Rap80 UIM linker defines selectivity by
optimizing avid binding across a single K63 linkage. We demonstrate that linkage-specific
avidity also underlies the selective preference for K48-polyUb in a tUIM protein with a shorter
linker, human ataxin-3. Using tUIMs as a model, we establish the general principles that
underlie linkage-specific avidity and polyUb affinity in multi-UBD proteins.
Rap80 UIMs bind monoUb weakly
To examine Ub and polyUb binding by Rap80, we produced a His6-tagged version of the
Rap80 tUIM peptide (Figure 1A) and fluorescently labeled it on a single cysteine that naturally
occurs in the C-terminal UIM sequence (Figure 1B). To resolve the contributions of the
individual UIM domains to binding, we created single-UIM peptides with either of the 8-
residue consensus Ub binding sites (UIM1 and UIM2, Figure 1A) deleted. Because these
peptides are small and relatively mobile in solution, binding to Ub or polyUb was detected as
an increase in the peptide fluorescence anisotropy. Control experiments showed that the
fluorescent label did not affect Ub binding (Supplementary Data Figure S1). Typical of UIM
domains (Fisher et al., 2003), UIM1 and UIM2 bound monoUb very weakly (KdUIM1 = 510
μM, KdUIM2 = 520 μM; Figure 1C). The tUIM peptide bound monoUb with the same low
affinity (KdtUIM = 520 μM). These results indicate that both UIM domains can interact with
Ub, and that binding of monoUb by the tUIMs is not cooperative.
Sims and CohenPage 2
Mol Cell. Author manuscript; available in PMC 2010 March 27.
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Page 3
Rap80 binds avidly across a single K63-linked diUb
Next we measured binding of the Rap80 UIM peptides to K63-linked diUb (K63-Ub2). The
single UIM peptides bound K63-Ub2 with roughly the same affinity as for monoUb (KdUIM1
= 230 μM and KdUIM2 = 470 μM for K63-Ub2; Figure 1D). In contrast, the affinity of the tUIM
peptide was much tighter (KdtUIM = 22 ± 1 μM for K63-Ub2). Binding data that included higher
ligand (K63-Ub2) concentrations fit a complex model of divalent-divalent interactions well,
with no change in the Kd value of interest (Figure S2).
To verify the binding constant measured by fluorescence anisotropy, we performed isothermal
titration calorimetry (ITC) of the Rap80 tUIM peptide with K63-Ub2. The ITC data neared
complete saturation and were fit by a single-site model (KdITC = 17.6 ± 0.7 μM, n = 0.97 sites;
Figure 1F), in excellent agreement with the anisotropy binding constant and supporting our
interpretation of those measurements. These data demonstrate a relatively high-affinity
interaction between the Rap80 tUIMs and K63-Ub2 that depends on both UIM domains,
indicating that the interaction is avid, with simultaneous binding to both Ub units in the K63-
Ub2. As a result of this avidity, Rap80 tUIMs show a 24-fold preference for K63-Ub2 over
Rap80 cannot bind avidly across a single K48-linked diUb
We next compared the affinities of the Rap80 UIM peptides for K48-linked diUb (K48-Ub2).
UIM1 and UIM2 bound K48-Ub2 with roughly the same affinity as the tUIM peptide
(KdUIM1 = 280 μM, KdUIM2 = 200 μM, KdtUIM = 157 ± 8 μM; Figure 1E). This indicates that
the interaction between the tUIM peptide and K48-Ub2 employs only one UIM domain and
therefore is not avid.
To confirm this result, we performed an ITC titration of K48-Ub2 with the tUIM peptide (Figure
1G). The ITC data reveal two distinct binding sites on K48-Ub2; both a negative and a positive
enthalpy interaction are evident. These data cannot be fit by a single-site model as used for the
avid K63-Ub2 interaction. Rather, the ITC data are fit well by a sequential binding model, with
the first-site affinity in good agreement with the anisotropy value (Kd1,ITC = 171 ± 40 μM),
and the second-site affinity in good agreement with the interaction of a single UIM with
monoUb (Kd2,ITC = 470 ± 140 μM). It is likely that the weaker interaction was not observed
in the fluorescence anisotropy titration because the ligand concentrations were lower than those
used in the ITC measurement. Overall, these data indicate that the Rap80 tUIMs bind avidly
to K63-Ub2, but not across a single K48 linkage. This linkage-specific avidity underlies a 7-
fold preference for K63-Ub2 over K48-Ub2.
Closely linked domains promote affinity and selectivity in avid polyUb interactions
To test the possibility that longer K48-linked chains could allow non-adjacent Ub units to
interact simultaneously with a single set of tUIMs, we measured the affinity of the tUIM peptide
for K48-linked tetraUb (K48-Ub4). The tUIM peptide bound K48-Ub4 more than eleven-times
tighter than K48-Ub2 (KdK48Ub4 = 14 μM), an increase in affinity indicative of avid binding
to Ub4. In spite of avid interactions with longer K48 chains, the tUIM peptide still bound K63-
Ub4 with a four-fold preference over K48-Ub4 (KdK63Ub4 = 3.6 μM). The decrease in Kd for
K63-Ub4 over K63-Ub2 can be understood because Ub4 contains more binding units in molar
terms, and because the longer chain presents a linear array of binding sites that may favor re-
binding to neighboring sites. These measurements indicate that avid binding to non-adjacent
Ub units is less efficient than avid binding across a single isopeptide linkage.
By the same reasoning, longer linkers between UIM domains should lower affinity and thus
lower linkage selectivity. To test this, we extended the 7-amino acid sequence between the
Rap80 UIMs to 14 residues (REVNSQEREVNSQE). This peptide bound K63-Ub2
Sims and CohenPage 3
Mol Cell. Author manuscript; available in PMC 2010 March 27.
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Page 4
substantially more weakly, and with very little selectivity (Table 1). Next we examined the
yeast tUIM protein Vps27, which has an unstructured 25-residue linker (Swanson et al.,
2003). As expected, the Vps27 tUIMs bound K63-Ub4 and K48-Ub4 indistinguishably. In
contrast, the Vps27 UIMs connected with a 7-residue linker bound K63-linked chains with
high affinity and specificity (Table 1). These results demonstrate that, for both polyUb chains
and linked UBDs, binding units tightly coupled in space provide the maximum opportunity for
high affinity and highly linkage-selective interactions.
Homology models of Rap80 bound to diUb reveal the structural basis of avid K63-polyUb
Multiple secondary structure prediction programs indicate that the Rap80 UIM domains are
strongly α-helical whereas the linker sequence is weakly α-helical. Circular dichroism (CD)
measurements support this (Figure S6 and S7). Figure 2B is a structural model based on these
predictions. We noticed that if the linking sequence acquired helical structure when bound to
K63-Ub2, the tUIM peptide would comprise one continuous α-helix with an integer number
of helical turns between the UIM domains. This would orient the Ub interaction surfaces of
both UIMs towards the same face of the peptide in a configuration ideal for simultaneous
interactions with both hydrophobic patches of the linear (Varadan et al., 2004) K63-Ub2
molecule. Next, we examined the five structures of UIM domains bound to monoUb (Hirano
et al., 2006; Swanson et al., 2003; Wang, et al., 2005). UIMs in all of these structures shared
a common orientation on the ubiquitin surface, along the axis created by the C-terminus and
K63 (Figure 2A). This would allow two UIMs on a single helix to simultaneously bind two
K63-linked ubiquitins. To model this Rap80·K63Ub2 interaction, we used the coordinates from
the NMR structure of Vps27 UIM1 bound to monoUb (Swanson et al., 2003) to place each of
the Rap80 UIMs on a Ub, and then connected the UIMs with an α-helical segment of
appropriate length. Remarkably, the resulting configuration of the bound ubiquitins easily
allows a K63 linkage and is a good match for the known K63-Ub2 structure (Figure S4). Figure
2D presents this model of Rap80 bound to K63-Ub2. Consistent with our binding data, we
could not model simultaneous UIM interactions to an open form of K48-Ub2, even with an
unstructured UIM linker (Figure S5).
To test directly the structural transition predicted for the Rap80 linker, we produced a version
of the Rap80 tUIM peptide without additional vector-derived residues and measured the CD
spectra of the peptide in the unbound and the K63-Ub2 bound state. Figure 2D shows the sum
of the unbound Rap80 and unbound K63-Ub2 spectra compared to the spectrum of Rap80 and
K63-Ub2 mixed at nearly saturating concentrations. Because large shifts in ubiquitin secondary
structure are typically not seen upon binding, the difference in the two spectra at 222 nm
indicates that Rap80 acquires significant helical structure when bound to K63-Ub2. Whole-
spectrum interpretations of these data indicate that the unbound form of Rap80 is 30-35% α-
helix, while the K63-Ub2 bound form is nearly 100% α-helix. Simpler estimates of helical
content predict less helicity (Figure S6). These data support a model for the Rap80·K63-Ub2
interaction in which the 7-residue linker becomes helical in the bound state and precisely
positions the UIM domains for avid binding across a single K63 linkage.
In our model of the Rap80·K63-Ub2 interaction, direct side-chain contacts between the Rap80
linker and ubiquitin are not predicted to be important. We tested 7 different 7 residue tUIM
linkers (Figure 2E) and found that they all supported high affinity, K63-specific polyUb
interactions, even though a wide variety of side chains were represented at each position within
the linker. This excludes a major role for direct linker side-chain·ubiquitin contacts in defining
affinity and linkage selectivity. Details on each of these constructs appear elsewhere in the
text. The common property of all these linkers is some degree of predicted helical content,
supporting our model of the Rap80·K63-Ub2 interaction.
Sims and CohenPage 4
Mol Cell. Author manuscript; available in PMC 2010 March 27.
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Page 5
Factors governing polyUb linkage specificity and affinity for tUIMs
We expected that tUIM binding properties would be affected by both the length and structure
of the linking sequence, and by the intrinsic affinities of the individual UIM domains for
ubiquitin. We constructed a set of peptides in which the Rap80 UIMs were linked by 1 to 9
alanine residues in order to examine linker length systematically in a way that was roughly
independent from the linker structure and UIM affinity. K63-Ub2 binding data for the alanine
linker set are presented in Figure 3A. The plot of Kd vs. linker length is well fit by a sine wave
that repeats every 3.5 residues, or approximately 1 α-helical turn (Figure 3B). This is consistent
with our model in which a single, continuous helix of tUIMs only engages the K63-Ub2
efficiently when the UIM binding sites are separated by a near-integer number of helical turns,
and thus face the same side of the helix. The optimum linker length for K63-polyUb binding,
7 residues, also most satisfies this rule (i.e., 6.9 turns between binding sites), whereas the
weakest binders (2A and 9A are >25-fold weaker than 7A) have linkers that keep the UIM
binding sites almost totally out of helical phase with each other (5.6 turns and 7.5 turns between
binding sites, respectively). This strong correlation demonstrates that α-helical linkers can
dramatically modulate the affinity for K63-polyUb by controlling the orientation of the UIM
domains with respect to each other, thus reducing or eliminating avidity.
We observed the same pattern of affinities when shorter linkers derived from the Rap80
sequence were tested (compare REVNSQ and REVAAQ to wild type in Table 1), indicating
further support for our model of K63-Ub2 bound Rap80. Even removing the linking sequence
entirely to position the binding sites in phase reduced K63-Ub2 affinity only 2-fold compared
to wildtype, whereas the 2-residue linker 'DM' binds K63-Ub2 with the weakest affinity of all
(Table 1). This may indicate some tolerance for variation in the lateral distance between UIMs,
but strict requirements for domain orientations. We note that affinity for K48-Ub2 was largely
independent of the linker length or composition in this data set and for the alanine linkers tested,
consistent with the finding that K48-Ub2 recognition is not avid (Table 1).
Intrinsic helical propensity in the linking sequence should correlate with tighter avid binding,
because more ordered linkers more effectively pre-organize the second receptor site for binding
after the first site is bound. In a striking example of this, Rap80 with the strongly helical 7-
alanine linker binds K63-Ub2 6-fold more tightly than Rap80 with the weakly helical wild-
type linker (Table 1; note no change in K48-Ub2 affinity). CD measurements confirm the
difference in helicity; in Figure 3C, note the marked difference in the extent of the trough at
222 nm, which is one measure of helical content.
When the UIMs from ataxin-3 were spliced together with the Rap80 linker, the result was a
much less helical peptide that was only weakly selective for K63-Ub2, and showed no K63
linkage preference for longer chains. When the same UIMs were joined by the more helical 7-
alanine linker, helicity and K63 selectivity were improved (Figure 3C, Table 1). This suggests
that there are minimal structural requirements for a linker in the context of its UIMs to achieve
linkage specificity through avidity. This also supports our model in which the Rap80 linker
defines selectivity through domain positioning, and not through specific contacts with Ub.
Using secondary structure prediction tools, we found several other linker variants predicted to
have more helical content than the wild-type Rap80 linker (Figure S7). Indeed, all of the linker
variants bound K63-Ub2 more tightly than wild-type Rap80, and the trends in affinity and
selectivity closely matched the trend in predicted linker helical content (see Table 1 entries for
S101A, S101E, DLKAAIQ and ARLEEKV linkers). CD measurements confirmed the
underlying difference in helical propensity for one set of these peptides, though the changes
were subtle (Figure S7). These results support our structural model of Rap80 bound to K63-
Ub2, and indicate that linker sequences that reduce the flexibility between UIMs promote high
affinity and linkage-selective interactions.
Sims and CohenPage 5
Mol Cell. Author manuscript; available in PMC 2010 March 27.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript


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Liverpool loanee paid his £50 fine in pennies and was sent back prompt
Vigouroux was menat to be on a season-long loan at the League One club
Welcome to the new Independent website. We hope you enjoy it and we value your feedback. Please contact us here.
Vigouroux was menat to be on a season-long loan at the League One club
The Blues have been inconsistent so far this season
'The Special One' is prepared for the ups and downs while his squad matures into a title winning side
Jose Mourinho has used the Spanish playmaker sparingly this season
The Italian was dismissed after just five games of the new season
Former Black Cat Kenwyne Jones relates how Stoke players developed the Paolo Di Canio feeling over ex-manager's 'sky football'
Tomato sauce is back at the Academy of Light, and players are once more allowed to use their mobile phones.
The Spaniard is believed to be concerned about his World Cup chances after slipping down Jose Mourinho's pecking order
The Blues take on Tottenham this weekend
The Blues set to take on Swindon as they look to progress further in the League Cup
Swindon are the first side to stand in the Blues' way
The League One side play a passing style of football
Chelsea face a run of four away games which Mourinho recognises will be a real test for his team
Mismanaged Rovers paid £3.5m in fees last season and narrowly avoided second straight relegation
Reds boss wants the Uruguayan to earn his place back in the squad after trying to force a summer move away from Anfield
Paolo Di Canio suspended the full-back
Manchester United play Liverpool in one of a number of all Premier League ties


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Cognitive Engineering Research Group (CERG)
CERG members study human-system integration in complex sociotechnical systems, focusing on critical care medicine, air traffic control, power systems, and air defence. CERG is associated with the Schools of ITEE, Psychology, and Medicine at UQ. The leader of CERG is Professor Penelope Sanderson.
We have a PhD scholarship now open in our group:
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Sanderson awarded UQ Award for Excellence in RHD Supervision
Penny Sanderson receives a UQ Award for Excellence in Research Higher Degree Supervision. Many thanks to all the wonderful CERG PhD students who submitted the nomination!
CERG at IEA2015 in Melbourne
All CERG's PhD students plus Penny Sanderson travelled to Melbourne for the International Ergonomics Association conference. All gave talks and all went very well.
Congratulations to Mike Pascale on PhD confirmation
Congratulations to Mike Pascale, who passed his PhD confirmation with many compliments from his readers. Mike is investigating the use of HMDs for healthcare applications.
Penelope Sanderson elected Fellow of International Ergonomics Association
Penny has learned that she has been elected a Fellow of the International Ergonomics Association (IEA). The award is formally presented at the IEA congress in Melbourne next month.


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Ron Washington To Get His Caddy Today
There's no game out at the Ballpark today -- shame too, since it's likely The Last Lovely Day before summer slams into high gear. Nevertheless, at 11:30 this morning Nolan Ryan and Texas Rangers manager Ron Washington will find themselves at home plate, where Ryan will give Washington the keys to a new Cadillac.
And not just any Cadillac, but a black '85 Biarritz just like the one New Orleans resident Washington lost during Katrina. Perhaps you recall: Back in January, during the Dr Pepper Mid-Winter Awards Banquet, the team told Washington he was getting the new car courtesy Moritz Cadillac, which helped the Rangers find the ride. Here's one that run $13,500. Quite the...steal? Hit it, Joe.
But, per the release just dispatched by John Blake: "Since that original announcement, the Cadillac has been refurbished and detailed by Moritz. The upholstery has been changed from red to white and a sunroof has been added to make the vehicle exactly as Washington's original model. The engine has also been replaced." My dad, a parts man from way back, says, sure, the thought's awful nice, but they shoulda just gone the whole Eldorado, "because giving him a Biarritz is like firing him."
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Blogs and Blogging Are you curious about blogging? And wanted to start blog of your own? Then read this expert guide by D Hari Babu (seo Trainer in Srihitha Technologies) to start a blog of your own and be a part of blogging world. How to start a blog or blogging? If you are familiar […]
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Local officials are scrutinizing public services from snowplowing to libraries to street sweeping as the latest analysis of property assessments shows declines in every community in Wayne, Macomb and Oakland counties.
In 50 cities or townships, property values dropped by double digits in the past year.
Houses sold after mortgage foreclosures are cited across the region as the biggest reason for the steep declines. ...


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Easter Season in Egypt, 1834: 'Smelling the Breeze', Making Kishk, Eating Colored Eggs & Salted Fish
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Posted in Colonial, Colonialism, Cuisine, Egypt, Food, Recipes Tagged Cairo, Copts, Easter, Edward William Lane, fasiikh, food, Kishk, Manners and Customs of The Modern Egyptians, Passover, shamm al-nasiim, Upper Egypt 3 Comments
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Forum - View topicPlease don't accept votes for unaired anime.
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Posts: 13784
Location: NZL
|Oh, why didn't you say so earlier? That's fine then.
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Shiroi Hane
Encyclopedia Editor
Posts: 6457
Location: Wales
|I must confess I don't think I actually ever looked at the Steins;Gate movie page itself -_-;;;
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Posts: 9
|I found S;G movie Bayesian's 105th
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Shiroi Hane
Encyclopedia Editor
Posts: 6457
Location: Wales
To re-iterate:
In fact, per this news article even that vague "fall" date is uncertain so I'm considering removing it anyway.
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Posts: 9
|Maybe some rich people watched S;G movie before theatrical release
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Crazy Fangirl
Posts: 7757
I hope you're being sarcastic, because all these votes are clearly from the staff working on the movie.
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Posts: 9
Before it released, it went up to TOP100
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Shiroi Hane
Encyclopedia Editor
Posts: 6457
Location: Wales
|Are you listening to what anyone is telling you at all?
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Posts: 15
Just to throw in my two cents. Running my own anime database, the current method ANN is using appears to be best by excluding anything rated before the vintage date.
The only other solutions would be to:
- include a field in the database for "not yet aired or aired."
Either solution would require a lot more database management which would also result in upset users when their votes aren't counted.
The entries that get these preemptive votes are typically so popular that once they do air their weight on the overall score is so minute, none if an exact date is given, it's not worth worrying about.
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Posts: 9
No need database fields change.
Sorry i am just writing understandable, Not in programming language.
Solution is
if (vintage date > NOW) or (vintage date is not decided) then NO seen some / seen and NO some / all links
/*If these links don't appear no one can vote anymore*/
But be aware if it is some old anime that already released but no known vintage date.
Old votes on unaired anime... mmm... don't delete these votes.
If you delete these many problems may appear.
I think it doesn't need so much database management.
Sorry my bad english
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Posts: 1039
@baljaa1718 I'll try to explain this in a simple manner.
The system accepts votes prior to something airing, yes?
However, once we have the air date: any and all votes before it aired are not counted.
This is a feature to catch people making unacceptable ratings.
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Shiroi Hane
Encyclopedia Editor
Posts: 6457
Location: Wales
|Not all anime have a vintage, for various reasons.
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Posts: 9
Sorry for posting in the old thread.
I see, I understand now. But unaired anime are not supposed to be TOP ANIME.
So i think you have to do some tweaks on ratings-anime.php only.
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The Mike Toole Show - Follow the Melos Brick Road
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Naruto, Vol. 72
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Answerman - Why Did Funimation's Attack On Titan Subtitles Fail?
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Overall score
would recommend to a friend.
Reviewer score
Would recommend
Perfectly located in a fascinating city
Call ahead and have them arrange for the airport shuttle, half price of taxi and no great delay. Friendly and eager to help staff, were able to store luggage for early arrival and start our museum hopping and restaurant sampling in Istanbul.
Breakfast was simple but adequate and was included to the rather reasonable room price. The only negative was the room size, which was very cramped, bath and shower tiny! However, clean , with free WiFi. The location more than made up for the cramped accommodations, waling distance from Aya Sofia, Blue Mosque, Topkapi, Grand Bazaar. Fun and safe to discover the old city
Would recommend staying, but would consider the deluxe rooms for an additional 30 Euros/day if room comfort is important.
Good buys in leather goods and carpets. Fun if you can bargain. Depending on establishment you final price should be 1/3 to 1/2 of asking price
Would not recommend
Will never do it again
The location is fantastic and the design as well, I was on honeymoon trip we need a room a king size bed 1st we shocked once we saw 2 beds besides one another so easily you can fall on the ground!! but the most things which upset me before
departure is when the reception guy ask me to pay for extra charges for soft drink from mini bar which we didn't touch or use!!! and then start to cry if we didn't pay for it he will pay I told him it not my mistakes we never touch or use it, anyway I was to hurry to catch my plan I pay but I promise to never back again, the stuff was miserable.
okay hotel
BE AWARE....that the reception desk does not know english very well at all!!!
They put me in a room that has windows to the courtyard where there is breakfast in the morning and the hallway door right outside mine. At night people smoking in the
courtyard penetrated through your windows. You could hear people talking and slamming the door and walking down the hall. I couldn't open the blinds to the windows because it would be face level to someone sitting in the courtyard. And forget it if you want to sleep in a little bit because like I said breakfast is in the courtyard and everyone eats out there.
Breakfast is not all free. Juices you have to pay for.
The airport shuttle that was suppose to pick you up at the front of the hotel was 30mins late and then you had to walk up the street and around to get to the shuttle. And the shuttle takes it's sweet time. My flight was at 12pm and I took the 835am shuttle and I barely made it in enough time to get to my gate. Remember you have to go through 3 different lines of security if you are flying internationally.
The location was great though and the bed was comfy.
There is a restuarant up stairs that the reception fails to tell you about and where it is. I went up there and no one was eating there.
While I was waiting for the late shuttle. I over heard a British woman complaining that someone was drilling all night and she couldn't sleep because it sounded like it was right outside her room. She also complained that her sink constantly fills up with water. I feel bad for her because I saw the reception desk and they had no idea what she was saying because they couldn't understand. They just nodded their heads and smiled. A little ridiculous.
Yes the Lira is cheap but I came to find out that nothing is cheap in Istanbul. They raised the prices so much that everything is really unaffordable or you know you could get cheaper in the states.
I had a guy in the bazzar try to sell me 1 silk scarf for 350TL!!! That's about $250. He told me (because I was with a friend of his) that they do this because of the tourist. But realize they don't go down in price much when you do bargain. Also, cruise ship tour people...be aware that the tour guides are paid off by some of the bazzar stores to have them take you to their store. Also, be aware that a lot of the stuff is made in China...so be careful what you buy if you want authenic.
As a single female....the turks are very insistant on asking you a question, wanting to walk with you, wanting to speak english with you...this is all a ploy...so be careful....it got so tiresome because they won't leave you alone...you basically have to ignore them or be rude.
2 star is the MAX ......
Front door is 4 stars hotel . AC didn't work in room
( staff sent ppl over to check but not to fix ) .
Noise & vibration because hotel is next to track( tram ) . We had a bad night expirence in Istanbul ( very lucky was ONE night stay only ).
5 EUR @ to or from Int'l airport is the most by suttle .
Avicenna - recommended
Avicenna is a great place to stay in Istanbul. Room was small but clean. Staff is extremely helpful, and the included breakfast was very good. Location is perfect - you can spend several days walking to many of the best attractions in the
city. Also very close to the tram, Bosphorus and great restaurants.
Quick walk to Hagia Sofia, Blue Mosque and several other attractions
Unbeatable location for the price!!!!
Wonderful location and staff!!! Hotel in walking distance to all the historical sites. Had a fabulous time and will definitely stay at the Avicenna Hotel again!
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£1,300 pcm
4 bedroom house to rent Norton Grange, Little Kineton, WARWICKA beautifully presented four bedroom home within the confines of the locally renowned Norton Grange. Little Kineton sits on the outskirts of Kineton Village which offers residents amenities including local high school, primary school, shops, outstanding butchers, doctors surgery, cafe, bank, publ... More details ›
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Red Clay Ramblers
Chapel Hill, NC
Venue Address (Get Directions)Concerts at the Historic Cooper's House of Oakland Mills (Circa 1790)
Date and Time
Saturday, August 18th, 2012 at 7:00pm
Age Limit
All Ages
For detail, invitation/reservation - Please contact Steve & Myra Gnadt at email@example.com
Now in their 40th year, the Tony Award-winning Red Clay Ramblers are a North Carolina string band whose repertoire reflects their roots in old-time mountain music, as well as bluegrass, country, rock, New Orleans jazz, gospel, and the American musical.
In 1993, Fool Moon on Broadway earned the Ramblers their second Drama Desk nomination for Outstanding Music in a Play, and Fool Moon in Los Angeles set box-office records; Fool Moon went on to run abroad in Vienna and Munich, returned to Broadway for a second success in late 1995, had a third Broadway run in 1998-99 and then enjoyed a 5-week run at the Kennedy Center in Washington, DC in 1999, and received a Special Tony Award that same year.
The Ramblers have been guests numerous times on "A Prairie Home Companion" and have appeared on numerous network and cable TV programs including the Tonight Show with Jay Leno. They have toured extensively in North America and in Europe, and have made tours to sub-Sahara Africa, North Africa and the Middle East. The Ramblers developed Kudzu: A Southern Musical, in collaboration with Pulitzer-winning cartoonist Doug Marlette, and staged the show at at Ford's Theatre in Washington, DC in 1998.
The Ramblers' Off Broadway run of Lone Star Love earned Outstanding Musical nominations from both the Lucille Lortel Awards and the NY Outer Critics' Circle, and the Original Cast Recording of the show was released January 2006 by PS Classics of New York. Their recording Fool Moon, the Music was released September 2007. The Red Clay Ramblers appeared with the North Carolina Symphony New Year's Eve 2007, and "Carolina Jamboree," their second ballet, launched by the Carolina Ballet in 2005, was reprised June 2008, and has been broadcast statewide numerous times over UNC Public Television.
The Daily Advance calls the Ramblers' latest CD, Old North State, "North Carolina culture at its best." Premier acoustic music station WNCW salutes The Red Clay Ramblers as "the house band of North Carolina." All over the world, The Ramblers continue to carry the banner of string-band music far and wide, and with great joy and zest!


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appellate courts
Civil Litigation
I would imagine brain scans of most adult prisoners -- particularly those with substance abuse issues or mental illness -- would show many of the same impulse control deficiencies that are revealed in the brains of adolescents. It seems that the issue should not be analyzed in terms of the age of the perp but the mental capacity of the perp.
In any event, I'm not sure whether impulse control issues in the brains of teens warrants an exception from tough sentencing for horrible crimes. For example, the overwhelming majority of teenagers -- even with their supposedly undeveloped brains -- still know enough not to whack people not only because it's legally wrong but because they have an innate sense of shared humanity.
"It seems that the issue should not be analyzed in terms of the age of the perp but the mental capacity of the perp."
This is a slippery slope . . . if an adult defendant became a crack or meth addict as a teen which prevented his brain from ever developing, should he then get a break for his "adult" crimes committed with a "teen" brain?
There is a very practical reason why we lock people up: Not for punishment, not for rehabiliatation, but to keep dangerous people segregated from society. I understand youthful impulsivity and developing minds, but even at 14 or 15 after you have been caught, you should have enough sense not to threaten a judge who decides your fate. This kid is incorrigible and needs to be locked up. In Illinois, we call it the John Wayne Gacy exception to the warrant requirement. This kid's sentence will stand.
I just read the AP article with the underlying facts of the case: the kid sounds like a freakin' psychopath, and to suggest that his only issue was having a 14-year-old brain at the time of his horrible crime is absurd. Indeed, even after committing this brutal murder, the cold-hearted punk had enough street cunning to attempt to intimidate witnesses into silence with threats of violence. Frankly, this kid sounds like he was born with the soul of a rabid dog.
This kid threw another kid off a 5-story parking garage to his death for no reason. By what argument does this kid deserve humane treatment? If he really deserves a chance for 'rehabilitation,' they could sentence him to 150 years with a chance for parole after 90 years. Considering his crime, that would be fair and would keep him away from society until he was no longer a significant threat to others.
The one thing that often gets overlooked when people are advocating for the criminal is society's right to be protected from that criminal. Is it sad that this young man's life was ruined by a choice he made when he was a child? Yes. But keeping society safe is far more important than giving this young man another chance.
The "brain development" of his dead victim is no longer operative. Why should society be put at risk on account of the tender age of the murderer?
Who cares about brain development anyway? I think that as long as young people do not have the rights of adulthood such as voting in particular, they should not have the responsibilities.
It is absurd that when a young person wishes to vote, we tell them that they are immature, irresponsible, and not capable of processing the necessary information, but when a y0oung person commits a crime, they are mature, level-headed, and clearly responsible.
That sounds like the drivult that Nortwestern's Child Law clinic Bernadine Dohrn (unrepentent terrorist and non-lwyer) came up with at a NU sponsored CLE that I attended. Your arguments may be valid for a kid who steals an I-Phone, but not for this kid. Just remember, the John Wayne Gacy exception to the warrant requirement. Do we let him go because the law says we should?
It's valid for *Everyone* As long as we decided that teenagers have the proper capacity to bear responsibility for crimes then they have the proper capacity to vote. It is an ageist double standard to insist otherwise.
Voting is not murder. We're talking about two sets of behavior that are orders of magnitude apart. Its not even apples and oranges, it apples and strange rocks from Pluto.
The best and last reason to keep him locked up for life is to prevent further harm to society. A person behind bars cannot reoffend. That is a factual certainty.
With the overwhelming research showing that the frontal lobe of the brain in adolescents is not yet fully developed, then throwing away the key on youth is inhumane. This is the part of the brain that controls impulse. In Canada, Omer Ninham would have been eligible for parole after a maximum of 10 years, even if sentenced as an adult. Ninham would still have to earn parole, it is not automatic or mandatory, but at least he would have the opportunity to make a life for himself. The recidivism rate in such cases is very low. When I read stories like this, I must admit that I stand proud as a Canadian.
"It is an ageist double standard to insist otherwise."
Drunk deep from the Kool-Aid you have.
@Joel Schultz
I hope that your perverted sense of justice is not common in Canada. Omer Ninham and a gang of 4 others taunted a much smaller Zong Vang using racist slurs, kicked him off his bike and threw the bag of groceries he had bought for his mother. They then chased him up a parking ramp, where they beat him, and then tossed Zong 5 stories to his death. Afterwords this "boy" threatened witnesses with violence and even threatened to rape one. I am sure something of this sort has happened in Canada before. I am proud to be an American, where an immigrant family like Zong Vang's family can get unyielding justice. In Canada how would you explain to their fearful family that in 10 years the animal that killed your son will be walking near you again?
The kid obviously committed a terrible crime... nobody is saying that he should walk free... but at the same time, he was 14... I'd like to see the results if he sat down with a shrink when he was 14 and then again as an adult. The amount of growth since then should dictate the length of the sentence.
Alan Katz
I like the way the blogger omits all mention of facts that led to the stiff sentence for this heinous crime. All 14-year olds know that you can't throw someone off a building for fun. LWOP is harsh but hopefully will be widely publicized and therefore have a deterrent effect on all but the most depraved. The perp is getting off easy - it's the victim and his parents that will never fully recover.
I'm in favor of this. There has to be some accountability. People have to be responsible for their actions. 14 years old or 18 years old, this person committed a murder and he doesn't deserve parole.
I'm in favor of this. There has to be some accountability. People have to be responsible for their actions. 14 years old or 18 years old, this person committed a murder and he doesn't deserve parole.
The United States is the only country that puts children away for life. We break every human rights treaty we have signed. WE violate several treaties and laws and do NOT hold ourselves accountable. 2500 children a year are serving life without parole. THE ONLY COUNTRY!!! What is wrong with you guys. At 14 years old children know they should not throw someone off a bridge for fun. The scientific proven problem is they do not have the brain to stop themselves when they are overwhelmed or in a peer motivated mode. That is why they are always taking risks and killing themselves in automobiles. They know they shouldn't drive fast, but they do it anyway. ALL other countries care for their children. People should be accountable and so should this child, if he has mental disorders HE SHOULD BE HELPED. I am with the Canadian on this one. You are all judging this child as an adult and as you think he should behave, not on the facts of adolescents and the human brain. It is cruel and unusual punishment. Period. The Supreme Court of the United States of America has already correctly ruled on the issue. Wisconsin wake up.
Most courts when dealing with juvenile offenders have them psychologically evaluated before trial and sentencing. Alot of these kids being charged as adults and punished with LWOP are sociopaths. There's no rehabilitating sociopaths and no way for them to function within society.
The Law Blog covers the legal arena's hot cases, emerging trends and big personalities. It's brought to you by lead writer Jacob Gershman with contributions from across The Wall Street Journal's staff. Jacob comes here after more than half a decade covering the bare-knuckle politics of New York State. His inside-the-room reporting left him steeped in legal and regulatory issues that continue to grab headlines.
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is One Chatty Farker
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When you get familiar with WOLF and realize its function, quality, market potential, you can buy it in quantity and sell on your own name and brand, by then I will quit direct selling by myself.
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Anything like ornamental grass, which is NOT digestible, can cause some severe gastro-intestinal disturbances, such as vomiting, diarrhea, bleeding, and possible blockages. If your dog doesn't show signs of normal behavior (eating, drinking, no vomiting or diarrhea) within the next 12-24 hours, you should have him examined ASAP.
Sometimes, undigestible stuff will pass the whole way through a dog's digestive system and not cause any problem...however, until you know that it has absolutely passed the whole way through, there still remains the possibility of a serious problem.
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Divers search reservoir on Ute reservation for evidence in homicide, sources say
Geoff Liesik, Deseret News
FORT DUCHESNE, Uintah County — The Utah Department of Public Safety dive team spent two days searching a reservoir on the Uintah-Ouray Indian Reservation, apparently looking for evidence in a federal homicide investigation.
Divers spent several hours Thursday and Friday working in the water along the eastern shore of Bottle Hollow Reservoir. The team was called in to assist the FBI and the Bureau of Indian Affairs in a joint investigation, according to DPS spokesman Dwayne Baird. He referred questions about the nature of that investigation to the FBI.
When contacted Friday, FBI spokesman Bill Facer reiterated the agency's long-standing policy of not offering comment on active investigations.
At least two DPS divers were seen entering the water Friday with metal detectors. They appeared to recover something from the water around noon, though it was unclear what the object was. The dive team, FBI agents and Bureau of Indian Affairs police left the reservoir without comment shortly after 12:30 p.m.
Several sources told the Deseret News the divers were looking for evidence in the shooting death of Evan Cuch. The sources, who spoke on condition of anonymity, would not disclose what that evidence might be or why investigators believed they would find it in the reservoir.
Cuch, 23, was shot at least once during a June 11 confrontation between two groups in Fort Duchesne's Little Chicago subdivision. He died a short time later at Uintah Basin Medical Center in Roosevelt.
A teenager was treated for gunshot wounds at the hospital as well, sources told the Deseret News. A second teen was apparently grazed by a bullet and did not require treatment.
Federal investigators arrested Grant Hubert Checora in connection with the shooting. He is charged in Ute Tribal Court with criminal homicide and an aggravated weapons offense.
Checora, 21, is being held in the Duchesne County Jail on the tribal court charges. The FBI and Bureau of Indian Affairs continue to investigate the case and are expected to present it to the U.S. Attorney's Office for possible prosecution in federal court.
This week's search marked the second time since the shooting that divers have entered Bottle Hollow looking for evidence. On June 13, divers with the Uintah County sheriff's search and rescue team searched the reservoir at the request of federal agents.
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NM farmers selling water to oil and gas developers
CARLSBAD, N.M. (AP) — With a scant agriculture water supply due to the prolonged drought, some farmers in Eddy County with supplemental wells are keeping bill collectors at bay by selling their water to the booming oil and gas industry.
The industry needs the water for hydraulic fracturing, known as fracking, the drilling technique that has been used for decades to blast huge volumes of water, fine sands and chemicals into the ground to crack open valuable shale formations.
In recent months, more legal notices have been appearing in the Current-Argus informing the public that a water-right holder with a supplemental well has submitted an application to the state engineer's office seeking to change the purpose of use from agriculture to commercial, or transferring the right from one location to another.
"A lot of folks are doing that," said New Mexico Interstate Stream CommLogin to read more
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YMMV: Tattooed Teenage Alien Fighters from Beverly Hills
- Fridge Logic: The kids' Sentinel forms are based off of constellations. One as seen from Earth. This makes sense for the team Nimbar assembled to protect Earth, but in one episode we hear about a previous group of Sentinels on another planet... who are still based on Earth's constellations (Orion, Draco, Ursa and Leo for those interested). How does that work?
- I Am Not Shazam: The team is actually known as the "Galactic Sentinels" within the show. The title is only mentioned during the first episode "In the Beginning...." as a joke to provide a Title Drop.
- So Bad, It's Good
- Suspiciously Similar Song: Compare the show's theme tune with "I Eat Cannibals" by Total Coelo.
- X Meets Y: Ultraman meets Mighty Morphin' Power Rangers to an extent.