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updated 08/23/2011 AT 10:00 AM ET
•originally published 08/22/2011 AT 9:50 PM ET
Jennifer Garner and Ben Affleck are getting ready for another baby!
The couple, who are parents to two daughters – Violet, 5½, and Seraphina, 2½ – are "thrilled" to be expecting their third child, they confirm to the Associated Press.
Garner and Affleck, both 39, have each spoken of being hands-on parents. After Seraphina's arrival, Garner said juggling acting and motherhood gave her a "split personality."


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Envision Capital's Cohen Discusses Bond Investing: Audio
Jan. 27 (Bloomberg) -- Marilyn Cohen, chief executive officer at Envision Capital Management, talks with Bloomberg's Pimm Fox and David Wilson about fixed-income investing and the bond market's performance.
(Source: Bloomberg)
This is a Bloomberg podcast. To download, watch or listen to this report now, click {1 <GO>}. For additional Bloomberg podcasts, see {BPOD <GO>}. -- Bloomberg News +1-212-617-4667 (Tony Dec/Zorovich)
Running time 19:38

subset: commoncrawl


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Veronica Lewis
Comments about Veronica Lewis
I am happy, yet all the same,
dreadfully disappointed.
I really, really, really love poetry-
and that truly scares me.
the thought that I,
the product of a long journey
trying to develop a rebellious,
anti- school, anti- learning persona,
could actually enjoy the monotonous


text:
Author has written 11 stories for Harry Potter.
Well, if you've read my stories, you might want to know a little bit about me.
My official pseudonym is Ginny G. Alridge, but you can call me Ginny. Or Evelina.
As for age...I'm 108!! No, seriously...well, I understand if you don't believe me. Not a lot of people do.
I'm not as obsessed with the Harry Potter books as I was, say, a year ago, but nevertheless the only fanfics I am inspired to write are Harry Potter ones.
J.R.R. Tolkien is an amazing author, and I'll kill anyone who says otherwise! Hmm...this already puts several critics on that particular hit list.
I do not own the Harry Potter series by J.K. Rowling. Those marvelous books are her property, and hers alone. Anything you recognize is NOT mine, but exclusively J.K. Rowling's.
Thank you.
Surprisingly enough, I like most canon pairings.
Lily/James, Ginny/Harry, Ron/Hermione, Neville/Luna, Rose/Scorpius
I absolutely HATE:
Neville/Hannah (I don't know why so don't ask), Ginny/Draco (ew - sorry if you like them, but ew), Fred/Hermione, George/Hermione (it just doesn't feel right), Harry/Hermione (they'll always be brother and sister)
Draco/Hermione is my guilty pleasure. I LOVE Ron/Hermione...but there's something intriguing about forbidden romance.
And, I'm sorry people, but, realistically, I think Sirius was too much of a player to actually have married anybody. I don't like fanfics where he falls in love.
And, again, I'm sorry, but I don't like fanfics where Lily has EXACTLY FOUR friends who each fall in love with one of the Marauders (or Frank). I mean, Siriusly? Where is the likelihood in that?
The Harry Potter Oath
I promise to remember Tonks each time time I knock something down.
I promise not to obey traffic laws for Sirius's sake of course.
I promise to remember Arthur whenever I am at St Mungo's Room.
I promise to remember Lily when I see someone who holds pure beauty.
I promise to remember Teddy when I see someone with turquoise hair.
I promise to remember Ginny whenever bogey hexes are unfurled.
Yes, I promise to love Harry Potter wherever I may go.
Awesome HP quotes:
Hermione - Who cares? I mean, it's sort of exciting, isn't it? Breaking the rules.
Ron - Who are you and what have you done with Hermione Granger?
Fred & George - (As Harry) Wow! We're identical!
Harry - Egypt, huh? What's it like?
Ron - Brilliant! Loads of cool stuff. Mummies, tombs. Even Scabbers enjoyed himself.
Hermione - You know, the Egyptians used to worship cats.
Ron - Yeah, along with the dungbeetle.
Hermione - (howls)
Harry - What are you doing?
Hermione - (howls again) Saving your life.
Harry - Thanks!...Great, now he's coming at us!
Hermione - Yeah, didn't think about that...RUN!
The girl you just called fat? She is overdosing on diet pills.
The girl you just called ugly? She spends hours putting on makeup hoping people will like her.
The boy you just tripped? He is abused enough at home.
See that man with the ugly scars? He fought for our country.
That guy you just made fun of for crying? His mother is dying.
Post this to your profile if you are against bullying. You never know what it's like until you walk a mile in their shoes.
I'm INTO THEATRE & ART, so I MUST be a homosexual.
Copy and paste this into your profile if you are against stereotyping.
Sorry, I usually try not to put these copy-paste thingies into my profile because it really clutters it up, but I couldn't resist for these ones:
If you cried during/after reading Harry Potter and the Deathly Hallows, copy this to your profile
If you're in denial over Tonks' and Lupin's deaths, copy and paste this into your profile.
If you loved DH, HBP, OotP, GoF, PoA, CoS, and SS/PS, and know what all those initials stand for, copy and paste this into your profile.
If you cried when Fred Weasley died (in Harry Potter and the Deathly Hallows), and are not afraid to admit it, copy-paste this on your profile.
If you cried when Dobby died (in Harry Potter and the Deathly Hallows), copy and paste this into your profile
If you want J.K.R. to make a series about the Marauders, copy this into your profile.
If you love Harry Potter, copy this into your profile.
If you love the Marauders AND think they are THE best, copy this into your profile.
If you liked Snape after Deathy Hallows, copy and paste this in your profile.
If you always mentally make the Sirius "serious" pun whenever somebody says, "I'm serious!" copy and paste this into your profile.
If you read Deathly Hallows in under a week, copy and paste this into your profile.
I only put a story in my favorites if I REALLY REALLY like it. As in if it makes me cry or if I laugh really hard reading it, or I think it's super-cute, etc.
If you want to comment on my stories or ask me something, PM me! As always. :)


text:
In early 2007, with violence in Iraq spiraling out of control, President Bush announced a surge of nearly 30,000 U.S. troops into the country. By year's end, the violence had declined markedly and Iraq had been put on a path toward stability.
Fast-forward two years, and President Obama decided on a troop surge for Afghanistan of like proportions. The top U.S. general in Afghanistan, Gen. Stanley A. McChrystal, had asked for up to 40,000 troops to supplement the 68,000 U.S. troops already there.
Another 35,000 troops have been provided by America's International Security Assistance Force (ISAF) coalition partners. As Mr. Obama weighed Gen. McChrystal's troop request, leaked to the public in September, some Republicans accused the administration of dithering. Administration spokesmen replied that the president was taking his time because he wanted to get the decision right after years of drift under his predecessor.
The proposition that a troop surge in Afghanistan would contribute to stabilizing the country, as it did in Iraq, rests on two myths: First, the surge in Iraq caused the population to turn against the insurgents. Second, the conditions that enabled the surge to succeed in Iraq also exist in Afghanistan.
Myth 1: The security gains from the surge in Iraq caused the population to turn against the insurgents. The history of the Iraq war shows that the troop surge in Iraq followed the grass-roots uprising in Al-Anbar province of the Sunnis against al Qaeda in Iraq (AQI). The uprising, also known as the Awakening movement, began in September 2006 with Sheik Sittar albu-Risha.
AQI had overplayed its hand with its heavy-handed imposition of Shariah law and mistreatment of the residents of that majority-Sunni province. Such mistreatment included the summary executions of tribal leaders who opposed AQI and the forced marriages of their daughters to AQI fighters. Sheik Sittar organized a resistance movement that spread across the province and became the Awakening.
Mr. Bush announced the troop surge from the Oval Office on Jan. 10, 2007. The surge involved sending five more Army Brigade Combat Teams (BCTs) and their associated support, along with about 4,000 Marines - nearly 30,000 troops in all. The additional five BCTs would make for 20 BCTs deployed in Iraq.
On Feb. 11, 2007, Gen. David H. Petraeus took command of the Multi-National Force-Iraq (MNF-I). That same month, the first surge BCT deployed. The other four BCTs deployed over the next five months. By July, the full surge force was in-country. Insurgent activity began to decline shortly thereafter.
Had there been no Awakening in 2006, the surge might have played out differently in 2007. The violence might not have declined, and Mr. Obama might not have the option of being able to consider shifting troops from Iraq to Afghanistan.
That brings up Myth 2, that the conditions that enabled the surge to succeed in Iraq also exist in Afghanistan. There has been no grass-roots Awakening movement in Afghanistan to rival that of Iraq. Nor does the United States have a reliable partner in the Afghanistan government of Hamid Karzai. The writ of the Karzai government hardly extends beyond the capital of Kabul.
The government's credibility has been badly damaged by charges that it stole the August presidential election. Election observers from the United Nations have reported that the election was marred with fraud on the part of Mr. Karzai's supporters. A runoff election had been scheduled - until the withdrawal of Mr. Karzai's challenger, former Foreign Affairs Minister Abdullah Abdullah. Now Mr. Karzai appears to have won re-election by default.
Regardless of whether Mr. Karzai or Mr. Abdullah is in charge, Afghanistan has never had a strong central government. Most Afghans live in villages - the country of 28 million is dotted with an estimated 30,000 villages - most of which have little connection with Kabul, due to the country's forbidding terrain and lack of a modern communications grid.
Authority in those villages is wielded by tribal leaders. Only 28 percent of Afghans over age 15 are literate, according to the CIA World Factbook. (The comparable figure for Iraq is 74 percent.)
Of course, no two countries are identical. But Iraq and Afghanistan are very different. Mr. Obama's order of a surge of troops into Afghanistan comes under circumstances very different from those of Iraq in 2007.
Christopher Duquette is an economist and spent six months in 2006-07 at Camp Victory in Baghdad with the U.S. military staff that works against improvised explosive devices He is also a Navy veteran of the 1991 Gulf War.


text:
Fwd: Compliance and Enforcement News Brief (HQ): EPA AnnouncesContest: "Help Fight Environmental Crime"
Mansel A NelsonEnvironmental Education Outreach Program928 523 1275
Begin forwarded message:
August 30, 2010
EPA Ann ounces Native American Youth Artwork Contest: "Help Fight Environmental Crime"
WASHINGTON – The U.S. Environmental Protection Agency (EPA) today announced a poster artwork contest for Native American students aimed at protecting the environment by encouraging the reporting of possible environmental crimes through EPA's tips and complaints website.
The contest, open to all middle and high school students who are members of a federally recognized tribe, is a chance for young people to draw their visions of environmental damage from their viewpoint. EPA will use the winning artwork on its website and on posters encouraging the reporting of environmental violations.
Special agents with EPA's criminal enforcement program will present plaques to winning entrants at their schools, and will give a presentation on what it is like to be an EPA criminal investigator.
The artwork will be judged on creativity, originality and how well it depicts the message of environmental violations. Entries must be received or postmarked by Oct. 8, 2010 to be considered. Winners will be announced in November. Students should submit artwork to:
U.S. Environmental Protection Agency
Criminal Enforcement – "Help Fight Environmental Crime" Contest
Denver Federal Center , Bldg 25, Ent. E–2
P.O. Box 25227
Denver , CO 80225
More information on the contest: http://www.epa.gov/compliance/criminal/contest/tribal.html
EPA Tips & Complaints website: http://www.epa.gov/tips
Note: If a link above doesn't work, please copy and paste the URL into a browser.
BP Oil Spill Response: Web site | Get email updates


text:
Southwestern Light
by Kevin Laundroche
A collection of Images over a 10 day period in October, 2009, from Northern Arizona and southern Utah.
Comment posted.
kateworks says
Kevin...gorgeous images and yes that magical Southwestern light..thanks for the beauty...K
posted at 05:03PM Nov 10 PST
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Blurb Sites


text:
Cytherea gives squirt shower to her whore with big splash
This legacy Video gallery is Unsupported !
Pichunter no longer supports video galleries. This page is here only for compatibility reasons and we do not guarantee anything working on this gallery page. If you want real free HD videos, visit our sister site Cliphunter.com


text:
The Milan Channel reported that discussions were taking place over a move back to the San Siro.
Kaka has not enjoyed his time at Real Madrid since leaving Milan and is looking for an exit from the Bernabeu.
More about...
What is your opinion? Feel free to discuss the article under. You have to comment by using your Facebook profile. Be fair and have respect for other people and opinions!
Who of Europe's top strikers will move to England this summer?
After a dreadful start to their Bundesliga campaign, Borussia Dortmund appear to have weathered the storm after going five matches without defeat.


text:
Click here to print the obituary below
Viola KELLY
KELLY, Viola Winnifred (Auntie Vi)
1919 – 2012
Peacefully at The Manoir Fleury, Viola passed away on December 6, 2012. Pre-deacesed by her three brothers Lawrence, Albert and Edgar. She will be lovingly remembered and greatly missed by her many nieces, nephews, cousins and friends. Her passion for travelling took her to many parts of the world and she loved and lived life to the fullest. The family welcomes all to a Celebration of her life on Thursday, December 13, 2012 at 11:00 at St.Veronica's Church, 1300 Carson, Dorval. In lieu of flowers, donations to the St.Veronica's Parish or charity of your choice would be appreciated. Funeral arrangements entrusted to J.J. Cardinal Funeral Home, 560 Lakeshore Dr., Dorval, (514) 631-1511,
Funeral Home
J.J. Cardinal
560 Lakeshore Dr.
Dorval, QC H9S 2B3
(514) 631-1511
Published in The Gazette on Dec. 11, 2012


text:
Flush With Cash, Right-Wing Extremists Train the Future Zealots of Pro-Israel Campus Activism
Sweden Is Moving Toward the 6-hour Workday — and It's Turning Out Great
Harvard's Prestigious Debate Team Loses to New York Inmates
Valeant Pharmaceutics Raised Prices on Needed Drugs, Then Its Stock Took a Hit
What the Classroom Didn't Teach Me About the American Empire
By Steven J. Ross $29.95
Jeremy Bernstein $14.82
Flickr / sirenmedia
Rupert Murdoch's international media group, News Corp., abandoned efforts to acquire British satellite broadcasting company BSkyB amid an outburst of official and public censure after it came to light that associated journalists spied on mobile phone conversations and bribed police officers to cover it up. (more)
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Are you James D Bangs?Claim your profile
Publications (40)159.25 Total impact
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ABSTRACT: The unfolded protein response (UPR) is a stress mechanism to cope with misfolded proteins in the early secretory pathway, the hallmark being transcriptional up-regulation of ER molecular chaperones such as BiP and protein disulfide isomerase. Despite the lack of transcriptional regulation and the absence of the classical UPR machinery, African trypanosomes apparently respond to persistent ER stress by a UPR-like response, including up-regulation of BiP, and a related spliced leader silencing (SLS) response whereby SL-RNA transcription is shut down. Initially observed by knockdown of the secretory protein translocation machinery, both responses are also induced by chemical agents known to elicit UPR in mammalian cells (H. Goldshmidt, et al. PLoS Path. 6:e1000731. 2010). As these findings were generated primarily in procyclic stage trypanosomes we have investigated both responses in pathogenic bloodstream stage parasites. RNAi silencing of the core translocon subunit TbSec61α, failed to induce either response. Interestingly, cell growth halted within 16 hours of silencing, but sufficient TbSec61α remained to allow full competence for translocation of nascent secretory proteins for up to 24 hours, indicating that replication is finely coupled with the capacity to synthesize and transport secretory cargo. Tunicamycin and thapsigargin at concentrations compatible with short term (4 hr) and long-term (24 hr) viability also failed to induce any of the indicators of UPR-like or SLS responses. Dithiothreitol (DTT) was lethal at all concentrations tested. These results indicate that UPR-like and SLS responses to persistent ER stress do not occur in bloodstream stage trypanosomes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.Eukaryotic Cell 08/2015; DOI:10.1128/EC.00118-15 · 3.18 Impact Factor
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ABSTRACT: The multivesicular body (MVB) is a specialized Rab7+ late endosome (LE) containing multiple intralumenal vesicles that function in targeting ubiquitinylated cell surface proteins to the lysosome for degradation. African trypanosomes lack a morphologically well-defined MVB, but contain orthologues of the ESCRT machinery that mediates MVB formation. We investigate the role of TbVps23, an early ESCRT component, and TbVps4, the terminal ESCRT ATPase, in lysosomal trafficking in bloodstream form trypanosomes. Both localize to the TbRab7+ LE and RNAi silencing of each rapidly blocks growth. TbVps4 silencing results in ~3-fold accumulation of TbVps23 at the LE, consistent with blocking terminal ESCRT disassembly. Trafficking of endocytic and biosynthetic cargo, but not default lysosomal reporters, is also negatively affected. Others reported that TbVps23 mediates ubiquitin-dependent lysosomal degradation of invariant surface glycoproteins (ISG65) (Traffic 2008, 9:1698). In contrast, we find that TbVps23 ablation does not affect ISG65 turnover, while TbVps4 silencing markedly enhances lysosomal degradation. We propose several models to accommodate these results, including that the ESCRT machinery actually retrieves ISG65 from the LE to earlier endocytic compartments, and in its absence ISG65 traffics more efficiently to the lysosome. Overall, these results confirm that the ESCRT machinery is essential in T. brucei and plays important and novel role(s) in LE function in trypanosomes.Traffic 07/2013; 14(10). DOI:10.1111/tra.12094 · 4.35 Impact Factor
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ABSTRACT: Mature dolichol-linked oligosaccharides (mDLOs) needed for eukaryotic protein N-glycosylation are synthesized by a multi-step pathway in which the biosynthetic lipid intermediate Man5GlcNAc2-PP-dolichol (M5-DLO) flips from the cytoplasmic to the luminal face of the endoplasmic reticulum (ER). The ER membrane protein Rft1 is intimately involved in mDLO biosynthesis. Yeast genetic analyses implicated Rft1 as the M5-DLO flippase, but since biochemical tests challenged this assignment, the function of Rft1 remains obscure. To understand the role of Rft1 we sought to analyze mDLO biosynthesis in vivo in the complete absence of the protein. Rft1 is essential for yeast viability, and no Rft1-null organisms are currently available. Here, we exploited Trypanosoma brucei (Tb), an early diverging eukaryote whose Rft1 homologue functions in yeast. We report that TbRft1-null procyclic trypanosomes grow nearly normally. They have normal steady-state levels of mDLO and significant N-glycosylation, indicating robust M5-DLO flippase activity. Remarkably, the mutant cells have 30-100-fold greater steady-state levels of M5-DLO than wild-type cells. All N-glycans in the TbRft1-null cells originate from mDLO indicating that the M5-DLO excess is not available for glycosylation. These results suggest that rather than facilitating M5-DLO flipping, Rft1 facilitates conversion of M5-DLO to mDLO by another mechanism, possibly by acting as an M5-DLO chaperone.Journal of Biological Chemistry 05/2013; 288(28). DOI:10.1074/jbc.M113.479642 · 4.57 Impact Factor
Article: Inhibition of nucleotide sugar transport in Trypanosoma brucei alters surface Glycosylation[Show abstract] [Hide abstract]
ABSTRACT: Nucleotide sugar transporters (NSTs) are indispensible for the biosynthesis of glycoproteins by providing the nucleotide sugars needed for glycosylation in the lumen of the Golgi apparatus. Mutations in NST genes cause human and cattle diseases and impaired cell walls of yeast and fungi. Information regarding their function in the protozoan parasite, Trypanosoma brucei, a causative agent of African trypanosomiasis, is unknown. Here, we characterized the substrate specificities of four NSTs, TbNST1-4, which are expressed in both the insect procyclic form (PCF) and the mammalian bloodstream form (BSF) stages. TbNST1/2 transport UDP-Gal/UDP-GlcNAc, TbNST3 transports GDP-Man, and TbNST4 transports UDP-GlcNAc, UDP-GalNAc and GDP-Man. TbNST4 is the first NST shown to transport both pyrimidine and purine nucleotide sugars and is demonstrated here to be localized at the Golgi apparatus. RNAi-mediated silencing of TbNST4 in PCF caused underglycosylated surface glycoprotein EP-procyclin. Similarly, defective glycosylation of the variant surface glycoprotein (VSG221) as well as the lysosomal membrane protein, p67 was observed in ΔTbNST4 BSF T. brucei. Relative infectivity analysis showed that defects in glycosylation of the surface coat resulting from TbNST4 deletion were insufficient to impact the ability of this parasite to infect mice. Notably, the fact that inactivation of a single NST gene results in measurable defects in surface glycoproteins in different life cycle stages of the parasite, highlights the essential role of NST(s) in glycosylation of T. brucei. Thus, results presented in this study provide a framework for conducting functional analyses of other NSTs identified in T. brucei.Journal of Biological Chemistry 02/2013; 288(15). DOI:10.1074/jbc.M113.453597 · 4.57 Impact Factor
Article: The Intracellular Trafficking and Glycobiology of TbPDI2, a Stage-Specific Protein Disulfide Isomerase in Trypanosoma brucei.[Show abstract] [Hide abstract]
ABSTRACT: Trypanosoma brucei protein disulfide isomerase 2 (TbPDI2) is a bloodstream stage-specific lumenal endoplasmic reticulum (ER) glycoprotein. ER localization is dependent on the TbPDI2 C-terminal tetrapeptide (KQDL) and is mediated by TbERD2, an orthologue of the yeast ER retrieval receptor. Consistent with this function, TbERD2 localizes prominently to ER exit sites, and RNA interference (RNAi) knockdown results in specific secretion of a surrogate ER retention reporter, BiPN:KQDL. TbPDI2 is highly N-glycosylated and is reactive with tomato lectin, suggesting the presence of poly-N-acetyllactosamine modifications, which are common on lyso/endosomal proteins in trypanosomes but are inconsistent with ER localization. However, TbPDI2 is reactive with tomato lectin immediately following biosynthesis—far too rapidly for transport to the Golgi compartment, the site of poly-N-acetyllactosamine addition. TbPDI2 also fails to react with Erythrina cristagalli lectin, confirming the absence of terminal N-acetyllactosamine units. We propose that tomato lectin binds the Manβ1-4GlcNAcβ1-4GlcNAc trisaccharide core of paucimannose glycans on both newly synthesized and mature TbPDI2. Consistent with this proposal, α-mannosidase treatment renders oligomannose N-glycans on the T. brucei cathepsin L orthologue TbCatL reactive with tomato lectin. These findings resolve contradictory evidence on the location and glycobiology of TbPDI2 and provide a cautionary note on the use of tomato lectin as a poly-N-acetyllactosamine-specific reagent.Eukaryotic Cell 11/2012; 12(1). DOI:10.1128/EC.00293-12 · 3.18 Impact Factor
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ABSTRACT: Recent advances in secretory biology of African trypanosomes reveal both similarities and striking differences with other model eukaryotic organisms. Secretion is streamlined for rapid and selective transport of the major cargo, VSG. Selectivity in the early and post-Golgi compartments is dependent on glycosylphosphatidyl inositol anchors. Streamlining includes reduced organellar abundance, and close association of ER exit sites with Golgi and with unique flagellar cytoskeletal elements that govern organellar replication and segregation. These elements include a novel centrin containing bilobe structure. Innate signals for post-Golgi sorting of biosynthetic lysosomal cargo trafficking have been defined, as have pathways for both biosynthetic and endocytic trafficking to the lysosome. Less well-defined secretory organelles such as the multivesicular body and acidocalcisomes are receiving closer scrutiny.Current opinion in microbiology 03/2012; 15(4):463-8. DOI:10.1016/j.mib.2012.03.002 · 5.90 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The biogenesis of the ER Exit Site/Golgi Junction (EGJ) in bloodstream-form African trypanosomes is investigated using tagged markers for ER Exit Sites, the Golgi and the bilobe structure. The typical pattern is two EGJ in G1 phase (1 kinetoplast/1 nucleus, 1K1N) through S-phase (2K1N), duplication to four EGJ in post-mitotic cells (2K2N) and segregation of two EGJ to each daughter. Lesser cell percentages have elevated EGJ copy numbers in all stages, and blocking cell cycle progression results in even higher copy numbers. EGJs are closely aligned with the flagellar attachment zone (FAZ) indicating nucleation on the FAZ-associated ER (FAZ:ER). Only the most posterior EGJ in each cell is in proximity to the bilobe, which is located at the base of the FAZ filament near the mouth of the flagellar pocket. These results indicate that EGJ replication in bloodstream trypanosomes is not tightly coupled to the cell cycle. Furthermore, segregation of EGJ is not obligately mediated by the bilobe, rather assembly of the EGJ on the FAZ:ER, which is coupled to the flagellar cytoskeleton, apparently ensures segregation with fidelity during cytokinesis. These findings differ markedly from procyclic-form trypanosomes, and models highlighting these stage-specific differences in EGJ biogenesis are proposed.Molecular Microbiology 12/2011; 82(6):1433-43. DOI:10.1111/j.1365-2958.2011.07900.x · 4.42 Impact Factor
Article: Late endosomal Rab7 regulates lysosomal trafficking of endocytic but not biosynthetic cargo in Trypanosoma brucei[Show abstract] [Hide abstract]
ABSTRACT: We present the first functional analysis of the small GTPase, TbRab7, in Trypanosoma brucei. TbRab7 defines discrete late endosomes closely juxtaposed to the terminal p67(+) lysosome. RNAi indicates that TbRab7 is essential in bloodstream trypanosomes. Initial rates of endocytosis were unaffected, but lysosomal delivery of cargo, including tomato lectin (TL) and trypanolytic factor (TLF) were blocked. These accumulate in a dispersed internal compartment of elevated pH, likely derived from the late endosome. Surface binding of TL but not TLF was reduced, suggesting that cellular distribution of flagellar pocket receptors is differentially regulated by TbRab7. TLF activity was reduced approximately threefold confirming that lysosomal delivery is critical for trypanotoxicity. Unexpectedly, delivery of endogenous proteins, p67 and TbCatL, were unaffected indicating that TbRab7 does not regulate biosynthetic lysosomal trafficking. Thus, unlike mammalian cells and yeast, lysosomal trafficking of endocytosed and endogenous proteins occur via different routes and/or are regulated differentially. TbRab7 silencing had no effect on a cryptic default pathway to the lysosome, suggesting that the default lysosomal reporters p67ΔTM, p67ΔCD and VSGΔGPI do not utilize the endocytic pathway as previously proposed. Surprisingly, conditional knockout indicates that TbRab7 may be non-essential in procyclic insect form trypanosomes.Molecular Microbiology 09/2011; 82(3):664-78. DOI:10.1111/j.1365-2958.2011.07842.x · 4.42 Impact Factor
Article: Amino Acid Determinants of Substrate Selectivity in the Trypanosoma brucei Sphingolipid Synthase Family[Show abstract] [Hide abstract]
ABSTRACT: The substrate selectivity of four Trypanosoma brucei sphingolipid synthases was examined. TbSLS1, an inositol phosphorylceramide (IPC) synthase, and TbSLS4, a bifunctional sphingomyelin (SM)/ethanolamine phosphorylceramide (EPC) synthase, were inactivated by Ala substitutions of a conserved triad of residues His210, His253, and Asp257 thought to form part of the active site. TbSLS4 also catalyzed the reverse reaction, production of ceramide from sphingomyelin, but none of the Ala substitutions of the catalytic triad in TbSLS4 were able to do so. Site-directed mutagenesis identified residues proximal to the conserved triad that were responsible for the discrimination between charge and size of the different head groups. For discrimination between anionic (phosphoinositol) and zwitterionic (phosphocholine, phosphoethanolamine) head groups, doubly mutated V172D/S252F TbSLS1 and D172V/F252S TbSLS3 showed reciprocal conversion between IPC and bifunctional SM/EPC synthases. For differentiation of zwitterionic headgroup size, N170A TbSLS1 and A170N/N187D TbSLS4 showed reciprocal conversion between EPC and bifunctional SM/EPC synthases. These studies provide a mapping of the SLS active site and demonstrate that differences in catalytic specificity of the T. brucei enzyme family are controlled by natural variations in as few as three residue positions.Biochemistry 09/2011; 50(41):8853-61. DOI:10.1021/bi200981a · 3.02 Impact Factor
Article: Cell-free Synthesis and Functional Characterization of Sphingolipid Synthases from Parasitic Trypanosomatid Protozoa[Show abstract] [Hide abstract]
ABSTRACT: The Trypanosoma brucei genome has four highly similar genes encoding sphingolipid synthases (TbSLS1-4). TbSLSs are polytopic membrane proteins that are essential for viability of the pathogenic bloodstream stage of this human protozoan parasite and, consequently, can be considered as potential drug targets. TbSLS4 was shown previously to be a bifunctional sphingomyelin/ethanolamine phosphorylceramide synthase, whereas functions of the others were not characterized. Using a recently described liposome-supplemented cell-free synthesis system, which eliminates complications from background cellular activities, we now unambiguously define the enzymatic specificity of the entire gene family. TbSLS1 produces inositol phosphorylceramide, TbSLS2 produces ethanolamine phosphorylceramide, and TbSLS3 is bifunctional, like TbSLS4. These findings indicate that TbSLS1 is uniquely responsible for synthesis of inositol phosphorylceramide in insect stage parasites, in agreement with published expression array data (17). This approach also revealed that the Trypanosoma cruzi ortholog (TcSLS1) is a dedicated inositol phosphorylceramide synthase. The cell-free synthesis system allowed rapid optimization of the reaction conditions for these enzymes and site-specific mutagenesis to alter end product specificity. A single residue at position 252 (TbSLS1, Ser(252); TbSLS3, Phe(252)) strongly influences enzymatic specificity. We also have used this system to demonstrate that aureobasidin A, a potent inhibitor of fungal inositol phosphorylceramide synthases, does not significantly affect any of the TbSLS activities, consistent with the phylogenetic distance of these two clades of sphingolipid synthases. These results represent the first application of cell-free synthesis for the rapid preparation and functional annotation of integral membrane proteins and thus illustrate its utility in studying otherwise intractable enzyme systems.Journal of Biological Chemistry 05/2010; 285(27):20580-7. DOI:10.1074/jbc.M110.127662 · 4.57 Impact Factor
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ABSTRACT: The surface of most protozoan parasites relies heavily upon lipid-anchored molecules, to form protective barriers and play critical functions required for infectivity. Sphingolipids (SLs) play important roles through their abundance and involvement in membrane microdomain formation, as well as serving as the lipid anchor for many of these molecules and in some but possibly not all species, as important signaling molecules. Interactions of parasite sphingolipid metabolism with that of the host may potentially contribute to parasite survival and/or host defense. In this chapter we summarize current knowledge of SL structure, synthesis and function in several of the major parasitic protozoan groups.Advances in Experimental Medicine and Biology 01/2010; 688:238-48. DOI:10.1007/978-1-4419-6741-1_17 · 1.96 Impact Factor
Article: Streamlined Architecture and Glycosylphosphatidylinositol-dependent Trafficking in the Early Secretory Pathway of African Trypanosomes[Show abstract] [Hide abstract]
ABSTRACT: The variant surface glycoprotein (VSG) of bloodstream form Trypanosoma brucei (Tb) is a critical virulence factor. The VSG glycosylphosphatidylinositol (GPI)-anchor strongly influences passage through the early secretory pathway. Using a dominant-negative mutation of TbSar1, we show that endoplasmic reticulum (ER) exit of secretory cargo in trypanosomes is dependent on the coat protein complex II (COPII) machinery. Trypanosomes have two orthologues each of the Sec23 and Sec24 COPII subunits, which form specific heterodimeric pairs: TbSec23.1/TbSec24.2 and TbSec23.2/TbSec24.1. RNA interference silencing of each subunit is lethal but has minimal effects on trafficking of soluble and transmembrane proteins. However, silencing of the TbSec23.2/TbSec24.1 pair selectively impairs ER exit of GPI-anchored cargo. All four subunits colocalize to one or two ER exit sites (ERES), in close alignment with the postnuclear flagellar adherence zone (FAZ), and closely juxtaposed to corresponding Golgi clusters. These ERES are nucleated on the FAZ-associated ER. The Golgi matrix protein Tb Golgi reassembly stacking protein defines a region between the ERES and Golgi, suggesting a possible structural role in the ERES:Golgi junction. Our results confirm a selective mechanism for GPI-anchored cargo loading into COPII vesicles and a remarkable degree of streamlining in the early secretory pathway. This unusual architecture probably maximizes efficiency of VSG transport and fidelity in organellar segregation during cytokinesis.Molecular biology of the cell 09/2009; 20(22):4739-50. DOI:10.1091/mbc.E09-07-0542 · 4.47 Impact Factor
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ABSTRACT: African trypanosomes are the causative agents of human trypanosomiasis (sleeping sickness). The pathogenic stage of the parasite has unique adaptations to life in the bloodstream of the mammalian host, including upregulation of endocytic and lysosomal activities. We investigated stage-specific requirements for cytoplasmic adaptor/clathrin machinery in post-Golgi apparatus biosynthetic sorting to the lysosome using RNA interference silencing of the Tbmu1 subunit of adaptor complex 1 (AP-1), in conjunction with immunolocalization, kinetic analyses of reporter transport, and quantitative endocytosis assays. Tbmu1 silencing was lethal in both stages, indicating a critical function(s) for the AP-1 machinery. Transport of soluble and membrane-bound secretory cargoes was Tbmu1 independent in both stages. In procyclic parasites, trafficking of the lysosomal membrane protein, p67, was disrupted, leading to cell surface mislocalization. The lysosomal protease trypanopain was also secreted, suggesting a transmembrane-sorting receptor for this soluble hydrolase. In bloodstream trypanosomes, both p67 and trypanopain trafficking were unaffected by Tbmu1 silencing, suggesting that AP-1 is not necessary for biosynthetic lysosomal trafficking. Endocytosis in bloodstream cells was also unaffected, indicating that AP-1 does not function at the flagellar pocket. These results indicate that post-Golgi apparatus sorting to the lysosome is critically dependent on the AP-1/clathrin machinery in procyclic trypanosomes but that this machinery is not necessary in bloodstream parasites. We propose a simple model for stage-specific default secretory trafficking in trypanosomes that is consistent with the behavior of other soluble and glycosylphosphatidylinositol-anchored cargos and which is influenced by upregulation of endocytosis in bloodstream parasites as an adaptation to life in the mammalian bloodstream.Eukaryotic Cell 08/2009; 8(9):1352-61. DOI:10.1128/EC.00156-09 · 3.18 Impact Factor
Article: T-Cell Responses to the Trypanosome Variant Surface Glycoprotein Are Not Limited to Hypervariable Subregions[Show abstract] [Hide abstract]
ABSTRACT: Variable subregions within the variant surface glycoprotein (VSG) coat displayed by African trypanosomes are predicted sites for T- and B-cell recognition. Hypervariable subregion 1 (HV-1) is localized to an internal amphipathic alpha helix in VSG monomers and may have evolved due to selective pressure by host T-cell responses to epitopes within this subregion. The prediction of T-cell receptor-reactive sites and major histocompatibility complex class II binding motifs within the HV-1 subregion, coupled with the conservation of amino acid residues in other regions of the molecule sufficient to maintain secondary and tertiary VSG structure, prompted us to test the hypothesis that Th cells may preferentially recognize HV-1 subregion peptides. Thus, we examined the fine specificity of VSG-specific T-cell lines, T-cell hybridomas, and Th cells activated during infection. Our results demonstrate that T-cell epitopes are distributed throughout the N-terminal domain of VSG but are not clustered exclusively within HV-1 or other hypervariable subregions. In contrast, T-cell-reactive sites were not detected within the relatively conserved C-terminal domain of VSG. Overall, this study is the first to dissect the fine specificity of T-cell responses to the trypanosome VSG and suggests that evolution of a conserved HV-1 region may be unrelated to selective pressures exerted by host T-cell responses. This study also demonstrates that T cells do not recognize the relatively invariant C-terminal region of the VSG molecule during infection, suggesting that it could serve as a potential subunit vaccine to provide variant cross-specific immunity for African trypanosomiasis.Infection and immunity 11/2008; 77(1):141-51. DOI:10.1128/IAI.00729-08 · 3.73 Impact Factor
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ABSTRACT: Sphingolipids are essential components of eukaryotic membranes, and many unicellular eukaryotes, including kinetoplastid protozoa, are thought to synthesize exclusively inositol phosphorylceramide (IPC). Here we characterize sphingolipids from Trypanosoma brucei, and a trypanosome sphingolipid synthase gene family (TbSLS1-4) that is orthologous to Leishmania IPC synthase. Procyclic trypanosomes contain IPC, but also sphingomyelin, while surprisingly bloodstream-stage parasites contain sphingomyelin and ethanolamine phosphorylceramide (EPC), but no detectable IPC. In vivo fluorescent ceramide labelling confirmed stage-specific biosynthesis of both sphingomyelin and IPC. Expression of TbSLS4 in Leishmania resulted in production of sphingomyelin and EPC suggesting that the TbSLS gene family has bi-functional synthase activity. RNAi silencing of TbSLS1-4 in bloodstream trypanosomes led to rapid growth arrest and eventual cell death. Ceramide levels were increased more than threefold by silencing suggesting a toxic downstream effect mediated by this potent intracellular messenger. Topology predictions support a revised six-transmembrane domain model for the kinetoplastid sphingolipid synthases consistent with the proposed mammalian sphingomyelin synthase structure. This work reveals novel diversity and regulation in sphingolipid metabolism in this important group of human parasites.Molecular Microbiology 09/2008; 70(2):281-96. DOI:10.1111/j.1365-2958.2008.06393.x · 4.42 Impact Factor
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ABSTRACT: The lysosomal/endosomal system of African trypanosomes is developmentally regulated and is important in the pathogenesis associated with infection of the mammalian bloodstream. Long considered to be a target for drug development, the internal pH of the lysosome has been variously reported to range from <5.0 to >6.0. We have refined a flow cytometric technique using a pH-sensitive probe that specifically targets the lysosome, tomato lectin:Oregon Green 488 conjugate. The probe is delivered to the lysosome with fidelity, where it is shielded against external pH. Measurement of fluorescent output in the presence and absence of lysomotropic agent (NH(4)Cl) then allows precise titration of steady state lysosomal pH (4.84+/-0.23). Using bafilomycin A1 to inhibit acidification we demonstrate that this method is responsive to pharmacological perturbation of lysosomal physiology. This work should facilitate future studies of the lysosomal function in African trypanosomiasis, as well as other parasitic protozoa.Molecular and Biochemical Parasitology 06/2008; 159(2):146-9. DOI:10.1016/j.molbiopara.2008.02.003 · 1.79 Impact Factor
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ABSTRACT: RNAi knockdown was employed to study the function of p67, a lysosome-associated membrane protein (LAMP)-like type I transmembrane lysosomal glycoprotein in African trypanosomes. Conditional induction of p67 dsRNA resulted in specific approximately 90% reductions in de novo p67 synthesis in both mammalian bloodstream and procyclic insect-stage parasites. Bloodstream cell growth was severely retarded with extensive death after > 24 h of induction. Biosynthetic trafficking of residual p67, and of the soluble lysosomal protease trypanopain, were unimpaired. Endocytosis of tomato lectin, a surrogate receptor-mediated cargo, was only mildly impaired (approximately 20%), but proper lysosomal targeting was unaffected. p67 ablation had dramatic effects on lysosomal morphology with gross enlargement (four- to fivefold) and internal membrane profiles reminiscent of autophagic vacuoles. Ablation of p67 expression rendered bloodstream trypanosomes refractory to lysis by human trypanolytic factor (TLF), a lysosomally activated host innate immune mediator. Similar effects on lysosomal morphology and TLF sensitivity were also obtained by two pharmacological agents that neutralize lysosomal pH--chloroquine and bafilomycin A1. Surprisingly, however, lysosomal pH was not affected in ablated cells suggesting that other physiological alterations must account for increased resistance to TLF. These results indicate p67 plays an essential role in maintenance of normal lysosomal structure and physiology in bloodstream-stage African trypanosomes.Molecular Microbiology 05/2008; 68(4):933-46. DOI:10.1111/j.1365-2958.2008.06195.x · 4.42 Impact Factor
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ABSTRACT: Translational control mediated by phosphorylation of the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha) is central to stress-induced programs of gene expression. Trypanosomatids, important human pathogens, display differentiation processes elicited by contact with the distinct physiological milieu found in their insect vectors and mammalian hosts, likely representing stress situations. Trypanosoma brucei, the agent of African trypanosomiasis, encodes three potential eIF2alpha kinases (TbeIF2K1 to -K3). We show here that TbeIF2K2 is a transmembrane glycoprotein expressed both in procyclic and in bloodstream forms. The catalytic domain of TbeIF2K2 phosphorylates yeast and mammalian eIF2alpha at Ser51. It also phosphorylates the highly unusual form of eIF2alpha found in trypanosomatids specifically at residue Thr169 that corresponds to Ser51 in other eukaryotes. T. brucei eIF2alpha, however, is not a substrate for GCN2 or PKR in vitro. The putative regulatory domain of TbeIF2K2 does not share any sequence similarity with known eIF2alpha kinases. In both procyclic and bloodstream forms TbeIF2K2 is mainly localized in the membrane of the flagellar pocket, an organelle that is the exclusive site of exo- and endocytosis in these parasites. It can also be detected in endocytic compartments but not in lysosomes, suggesting that it is recycled between endosomes and the flagellar pocket. TbeIF2K2 location suggests a relevance in sensing protein or nutrient transport in T. brucei, an organism that relies heavily on posttranscriptional regulatory mechanisms to control gene expression in different environmental conditions. This is the first membrane-associated eIF2alpha kinase described in unicellular eukaryotes.Eukaryotic Cell 12/2007; 6(11):1979-91. DOI:10.1128/EC.00249-07 · 3.18 Impact Factor
Article: Multiple Motifs Regulate Trafficking of the LAMP‐Like Protein p67 in the Ancient Eukaryote Trypanosoma brucei[Show abstract] [Hide abstract]
ABSTRACT: p67 is a lysosome-associated membrane protein-like lysosomal type I transmembrane glycoprotein in African trypanosomes. The p67 cytoplasmic domain (CD) is both necessary and sufficient for lysosomal targeting in procyclic insect-stage parasites. The p67CD contains two [DE]XXXL[LI]-type dileucine motifs, which function as lysosomal targeting signals in mammalian cells. Using a green fluorescent protein fusion to the p67 transmembrane and cytoplasmic domains as a reporter system, we investigated the role of these motifs in lysosomal targeting in procyclic trypanosomes. Pulse-chase turnover studies, steady-state immunolocalization and quantitative flow cytometry all gave consistent results. Mutagenesis of the membrane-distal dileucine motif impairs lysosomal trafficking leading to partial appearance of the reporter on the cell surface. Mutagenesis of the membrane-proximal motif has little effect on proper targeting. Simultaneous mutagenesis of both motifs results in quantitative delivery to the cell surface. Thus, the distal motif plays a dominant role, but both dileucine motifs are necessary for maximal lysosomal targeting. Additional studies suggest that the upstream acidic residues in each motif influence lysosomal targeting and may also affect forward trafficking in the early secretory pathway. These results strongly suggest an evolutionary conservation in lysosomal trafficking mechanisms in the ancient eukaryote Trypanosoma brucei.Traffic 09/2007; 8(8):1007-17. DOI:10.1111/j.1600-0854.2007.00588.x · 4.35 Impact Factor
Article: De Novo Sphingolipid Synthesis Is Essential for Viability, but Not for Transport of Glycosylphosphatidylinositol-Anchored Proteins, in African Trypanosomes[Show abstract] [Hide abstract]
ABSTRACT: De novo sphingolipid synthesis is required for the exit of glycosylphosphatidylinositol (GPI)-anchored membrane proteins from the endoplasmic reticulum in yeast. Using a pharmacological approach, we test the generality of this phenomenon by analyzing the transport of GPI-anchored cargo in widely divergent eukaryotic systems represented by African trypanosomes and HeLa cells. Myriocin, which blocks the first step of sphingolipid synthesis (serine + palmitate --> 3-ketodihydrosphingosine), inhibited the growth of cultured bloodstream parasites, and growth was rescued with exogenous 3-ketodihydrosphingosine. Myriocin also blocked metabolic incorporation of [3H]serine into base-resistant sphingolipids. Biochemical analyses indicate that the radiolabeled lipids are not sphingomyelin or inositol phosphorylceramide, suggesting that bloodstream trypanosomes synthesize novel sphingolipids. Inhibition of de novo sphingolipid synthesis with myriocin had no adverse effect on either general secretory trafficking or GPI-dependent trafficking in trypanosomes, and similar results were obtained with HeLa cells. A mild effect on endocytosis was seen for bloodstream trypanosomes after prolonged incubation with myriocin. These results indicate that de novo synthesis of sphingolipids is not a general requirement for secretory trafficking in eukaryotic cells. However, in contrast to the closely related kinetoplastid Leishmania major, de novo sphingolipid synthesis is essential for the viability of bloodstream-stage African trypanosomes.Eukaryotic Cell 03/2007; 6(3):454-64. DOI:10.1128/EC.00283-06 · 3.18 Impact Factor
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University at Buffalo, The State University of New York
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For things to change we need to change the system of government. Our current system of voting for party's is bankrupt. It encorages nepotism and cronyism and these abuses just get worse. When there is no accountablilty to the voters. When the party and influence in the party is the most important thing. Coruption rules. WE NEED DIRECT LOCAL ELECTIONS FOR THE KNESSET. LET THE MK'S BE ACCOUNTABLE TO THE PEOPLE.
from the article: Taking Stock / Peeing in the pool


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Our 3 1/2" to 4" plastic dinosaurs come in assorted styles and colors. Dinosaurs are a kid favorite and perfect for young paleontologists and perfect for loot bag treats. These assorted size plastic dinosaurs are sold by the dozen. Please order in increments of 1 dozen.
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- Right size dinosaurs Review by tex
the dinosaurs were hidden in a dirt pile the 4 and 5 year old boys play in at our house. It became a "wonder" how they appeared there. The bright colors and the size were just right for the explorers.
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DIY Halloween Door Decorations
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5 Steps to Keeping Zombies Away During the Zombie Apocalypse
In the event that a Zombie Apocalypse does occur, you and your loved ones will remain safe and untouched when you follow these (fun and) simple steps.
Party Tips on Hosting a Kid-Friendly Halloween Party
Teachers and moms, use these Halloween decorating and party planning tips in order to throw a fabulous Halloween party this year!


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Granite Ridge Chenin Blanc
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Special Offer: €10.00Chenin is an extremely versatile grape, able to make excellent wines in many different guises, from Late Harvest to Straw wine to classic dry Chenin and even Méthode Champenoise. This example is a wonderfully lively, intense, fruity and beautifully balanced dry Chenin Blanc. With flavours of citrus, sherbet and flint, there is plenty of leesy mouthfeel and purity of fruit, finishing with a refreshing lime and mineral length. A true noble varietal, Chenin deserves to be taken seriously – even for everyday drinking wines such as this. Learn More
Tire Bouchon Cote du Rhone 2013
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Special Offer: €10.00Good rich red colour. Has an air of quietly sweet blackberry jam – step forward the Syrah influence, with a sprinkle of licorice and garrigue dust. The bouquet gives a sense of southern sun, without excess power. The palate is enjoyably fruity and forward, delivers its bundle right away before toning down, leading to a tighter, darker grip. Easy to like and appreciate. Learn More
Granite Ridge Shiraz
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Special Offer: €10.00Granite Ridge Shiraz focuses on using natural methods to achieve a balanced style that promotes drinkability, but also releases flavour, varietal spiciness, individuality, balance and freshness. These are the great, innate attributes of Shiraz. The ultimate test is: can you drink a bottle on your own? Answer this when you're onto your second one! Learn More
Georges Duboeuf Beaujolais Nouveau 2015
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Available for pre-order only, for delivery on and after Thursday, 19th November. Learn More
Hirschvergnügen Grüner Veltliner 2013
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Special Offer: €15.95Wonderfully fresh, grassy nose, delicate citrus aromas; the scent of a freshly sliced apple - only more intense. Juicy and fruity on the palate, with cheerful acidity, crisp and lean, packed into an easy-drinking style Learn More
Radford Dale Chardonnay 2013
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Special Offer: €17.30With strong influence of Alex Dale's upbringing in Burgundy, the making of this Chardonnay is also adapted to our particular conditions in this ocean-facing part of the Cape –our mineral geology and temperate maritime climate both lending much to the equation. Restraining acidity, citrus flavours and integrated oaking the introverted side of its character; ripeness and great persistence on the palate the more expansive side. Genuinely mineral, with a crisp lime-citrus edge and an elegant yet intense durability. A Chardonnay striving to be individual, focused on backbone not butter.
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Radford Dale Black Rock 2013
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Special Offer: €17.50The vibrant and immensely lively purple hue of this wine shines out of the glass. Its intricate spice, liquorice and wild bush ("fynbos") aromas grab your curiosity, while preparing the senses for a highly individual and aromatic palate. A vinous and wonderfully charged mouthfeel combines with an array of lavender, spices, strawberries and even blueberries. A distinctly mineral quality pervades through the palate, creating lift and freshness in the flavours and a subtle, full and crescendo finish. A highly individual, enigmatic wine; a true blend. As refreshing in its palate as it is in its approach Learn More
Chateau de Pierreux Brouilly Reserve du Chateau 2011
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Special Offer: €19.95From one block of 80 year old vines - La Roche. 100% de-stemmed, 3 weeks maceration and elevage in 2-5 year-old barrels ex the Clos du Tart,for 18 months. Deep, almost impenetrable colour. Equally deep and serious nose. The palate shows plenty of tannin and an incredible depth and wealth of black-skinned fruit. Impressive hardly does justice to this wine, Beaujolais on steroids! Learn More
Quinta do Perdigao Dao 2009
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Special Offer: €20.00Quinta do Perdigao is one of the finest wineries based on the Dao River. Its wines often fit the stereotype of a Dao wine, said to be hard, cold, tannic, delicious and monstrous! Getting into the details, dark fruits - blackberry, cherries, plums and some French "garrigue" and black pepper. A sexy, elegant and sensual wine. Learn More
Quinta do Perdigao Encruzado White 2013
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Special Offer: €20.50According to Wines of Portugal, the grape, Encruzado, "is one of Portugal's absolutely top white grape varieties. The best examples have delicate aromas of roses and violets, light citrus notes, a touch of resin and, in certain conditions, intensely mineral notes. Amongst its virtues is the ability to maintain almost perfect balance between sugar and acidity, making serious, rich, structured wines with extraordinary ageing potential. It is used both as a single variety and as a star ingredient in many Dão blends. The Encruzado vine yields well, presenting no major problems in the vineyard." Learn More


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Cold Winds in Cairo
From Paul Sullivan On November 26, 2012 · Leave a Comment
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When I was living in Cairo, the transition to winter was sometimes smooth. The beastly oven of summer changed slowly into a bearable fall of cool-warm. The fall moved from the cool-warm to a few weeks of cold, or at least what was cold to Egypt. These were smooth changes. It [...]
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Heather Removes Richie From her Life
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The Scoop is reporting that Heather Locklear is removing her Richie tattoo and supposedly replacing it with a Finch one. Finch was Spade's character from "Just Shoot Me." Somehow I doubt that they are so in love that she is ready to make another permanent marking on her body. Especially since they are not even really going public yet based on the amount of space between them as they left Maestro Restaurant in Beverly Hills last night.
Heather Locklear
David Spade
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amisarcastic 9 years
love spade !!!! am sure he is a clean guy. just not interested in making the best dressed list. as long as he is good to her & daughter thats the name of the game.
MissSixty1 9 years
she is sooo beautiful!!! i doubt this story about the her tattoo is infact correct espeically the part where she is apparently chaning it to FINCH...and if this is true...shes making a big mistake...to ink someone's name on your body is such bad luck lol
My-Opinion 9 years
wants to rescue evil doers kitten* lol~
PrincessPixie 9 years
You could not pay me enough to ink someone's name on my body.
stone_soup 9 years
yeah, kidcrazy2. i think it's dumb, especially if you were married to someone like tori spelling or if you live in Hollywood.
kidcrazy2 9 years
Yet another reason NOT to permanently ink some guy's name onto your body.
jennifer76 9 years
Eeeeeeeeew, quick...over to the 100 Hotties list to get that imagery OUT of my head! :) LOL
JennaV 9 years
Yup jen...he is so icky. I have a hard time she would let him touch her in her nether regions.... Is this our generations Cindy Mancini and Ronnie Miller...LOL!!!!!
jennifer76 9 years
I get this "Can't Buy Me Love" vibe from this whole thing. What exactly does she owe the man? Prolly not $1,000 that he was supposed to spend on a telescope...but something.
TruJrzyGirl 9 years
It's interesting how he is walking many steps a head of her and she has this smirk on her face ... It's also interesting hwo people hook up in Hollywood and two weeks later they are being labeled, "Oh so serious" ... LOL !!! I could be dating someone 6 months and maybe if he is lucky, I'm just getting ready for "Exclusive" ... Whatever happen to Look Before You Leap?? In Hollywood it's jump with both feet and don't look at al ... Just act later !
Toronado 9 years
I've always thought tattooing someone's name on yourself is foolish. But isn't Heather a little too old to be getting tattooes, anyway...or do I need to 'get wid da times, yo'?
pink_magnetism 9 years
Getting a new tattoo of Finch for David Spade would be a stupid idea. Then again, I think tattooing anyone's name on your body is a stupid idea. Especially on your woohoo, as jleegirl suggested Heather's is.
lovesDWade 9 years
I will be soooo over Heather if she did this, since she is one of my favs!
ethiopian_princess 9 years
JK Boogie, love AJ and Johnny. Love their tattoos. After Billy Bob, AJ learned better than to tattoo some lover's name on her body. LOVE the prayer on her back from when she adopted Maddox. THAT is meaningful. I don't think Johnny has Vanessa Paradis' name tattooed on his body. Jude Law had to get "Sexy Sadie" removed when he and ex-wife split. Charlie had to get "Denise" removed. When Tori and Dean break up, he has to get her mug and her name removed from his arms. Instead of (insert mater du jour's name here), why not get something meaningful like for beating addictions, a parent, a grand parent, a dog, the nanny, the house keeper, for reaching 95 lbs, etc. as all those things last longer than a Hollywood marriage.
PoohBare 9 years
Kind of makes you wonder about David, like why does attract such hot ladies. He has had relationships with several hot models and actresses. I wonder if he is good in bed or something? Somehow I always felt like Heather would end up with Tommy Lee again, ugh!
mle983 9 years
i like how stevo has "your name" tatooed on himself...so when a chick asks what his tat says he can just say "your name". funny...but dumb
JK-Boogie 9 years
Tattoos...always seem like a great idea at the time...See Angelina and Johnny Depp.
new2man 9 years
She's beautiful, he looks like he should be begging for change on the corner. Doesn't mean he isn't a nice guy, but he could stand to clean up a little.
Padraigin 9 years
Why on earth would she get a tattoo of a character name from a defunct tv show? I'll believe that when I see it.
FB1977 9 years
No way will they last...I think he's way more into her than she is into him. He's just making her feel good following a nasty breakup. Whatever happened to taking some time OFF, some alone time Heather? So unhealthy to drown your sorrows in another man...*sigh*
TriniBaby81 9 years
Remove the old tattoo but for Pete's sake, don't get that one. Who knows if they'll even last. He is funny, so at least they can have fun together.
My-Opinion 9 years
haha@ fatole pornstar* yuck, reminds of the few movies we found as a kid in my parents drawers (yes we snooped) and that big fat hairy dark dude with the black hair and super thick mustache was starring in it.........aaaaahhhhhhhhhh shudders*
pinkangelmonkey 9 years
haha whatever richie=ron jeremy!
babybeluga 9 years
LOVE David Spade.
My-Opinion 9 years
D. Spade certainly isn't the best looking guy, or really not even a good looking guy but he's obviously got something., and i'm thinking it's sincerity and patience. ANYthing is better than that richie dude, ugh~


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Media Bureau Events and Workshops
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Below is a listing of the tutorials the Media Bureau has conducted on a variety of subjects.
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Live updates
Pardew buries hatchet and praises 'great' Wenger
Crystal Palace manager Alan Pardew has described Arsene Wenger as one of the great managers ahead of their Premier League match-up on Sunday.
Palace take on Arsenal at Selhurst Park hoping to extend a poor start to the season for the Gunners, who suffered a shock defeat to West Ham on the opening weekend.
The pair clashed on the touchline in 2006 when Wenger took exception to Pardew celebrating a last-minute winner for West Ham, and Pardew has also criticised the Frenchman for failing to promote English players.
The Eagles boss insists those disagreements after now firmly in the past.
"On the touchline we all have run-ins - we've both been in this game a long time," he said.
"In my opinion he is one of the great managers, absolutely. I don't think we'd ever seen the type of football played in the Premier League before Arsene.
"He definitely educated coaches and managers in this country on the beauty of possession.
"He's a very brave manager to play the way he does week in week out and stick to the principles he has.
Palace boss Pardew takes no pleasure in overtaking Newcastle
Crystal Palace boss Alan Pardew says he takes no satisfaction from seeing his new club overtake former employers Newcastle in the league.
Pardew has taken the Eagles from the relegation zone to now being one place above Newcastle.
Alan Pardew gives thumbs up to winter 2022 World Cup
Crystal Palace boss Alan Pardew claims a winter World Cup in Qatar in 2022 will help England's chances and that the world of football must just deal with any disruption the change in dates will cause.
The final decision on the dates will be made by FIFA on March 20, and Pardew said playing in Qatar in the summer would not have been right.
Newcastle United manager Pardew also fined £60,000
Newcastle United manager Alan Pardew was also fined £60,000 and warned as to his future conduct. The Football Association said an Independent Regulatory Commission ruled the first three-matches imposed are a stadium ban with the remaining four a touchline ban.
The ban comes after he was involved in an incident in which he appeared to headbutt a Hull City player in the 72nd minute of a fixture on March 1.
Newcastle's Alan Pardew banned for seven matches
Newcastle United manager Alan Pardew will serve a seven-match suspension with immediate effect, subject to any appeal, after he admitted an FA misconduct charge.
Alan Pardew charged with improper conduct by FA
Newcastle United manager Alan Pardew has been charged by the FA with improper conduct after headbutting a Hull City player.
The FA said it was a "non-standard case" because of its "serious, violent and/or aggressive nature."
Pardew has until 6pm on Thursday to respond to the charge.
Shepherd: Headbutt shame will follow Pardew for life
Freddy Shepherd has told ITV News Tyne Tees that the controversy surrounding Alan Pardew's headbutt of Hull's David Meyler will follow him for the rest of his life.
Speaking about the incident, the former Newcastle United owner said: "He has got that for the rest of his life. That will be on his back for the rest of his life."
In the interview, Freddy Shepherd also made it clear that he does not want to see Alan Pardew lose his job.
Fans in Newcastle react to Pardew 'head-butt' incident
Football pundits condemn 'head-butt' by Pardew
Former England international and Match of the Day presenter Gary Lineker and pundit Richard Keys took to social media to condemn Newcastle manager Alan Pardew, who was fined £100,000 by his club after he appeared to headbutt Hull midfielder David Meyler.
Newcastle fine Alan Pardew £100,000 after 'head-butt'
Newcastle manager Alan Pardew has been fined £100,000 and received a formal warning from the club in relation to his behaviour at Hull, the north-east club have announced.
The club said they are "disappointed with the actions of its manager."
In a statement the club said: "His behaviour was unacceptable and is not the behaviour we expect from the manager of Newcastle United."


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Holden Montage Beanie (Women's)
Product Details
Style: 31119
The dye on the Holden Montage Beanie is unique and gives this women's hat an almost tie-dyed look. The chunky knit keeps ears warm, and the pom-pom on top adds festive style.
- 100% soft acrylic knit
- Multicolored pom-pom detail
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Mikveh Israel Congregation and Cemetery
Oldest Jewish congregation in Pennsylvania
The Experience
Mikveh Israel worships in a building not far from its original 1782 redbrick structure on Cherry Street. Visitors can see representations of three of its synagogues in a tapestry that hangs in front of the sanctuary. Reminders of the congregation's long history include a marble reader's stand (tebah) from the congregation's 1859 home, and two sets of Torah ornaments (Rimonim) made by colonial silversmith Myer Meyers.
The cemetery on 8th and Spruce Streets, part of Independence National Historical Park, includes the grave of Rebecca Gratz, reportedly the inspiration for the character Rebecca in Sir Walter Scott's Ivanhoe, and memorials to Haym Salomon, who helped finance the American Revolution.
Mikveh Israel traces its beginning to 1740, when Thomas Penn granted land to Nathan Levy for a burial ground for Levy's infant son. There, Levy established a cemetery for the Jewish community. In 1782, Mikveh Israel's first house of worship was completed with financial assistance from Benjamin Franklin and others. The synagogue has moved several times before returning to its original neighborhood in 1976 where it serves a vibrant congregation, faithful to its tradition.
Other Information
Synagogue visiting hours: Open Sun – Fri (call for exact times)
Service times: Daily at 7:15 a.m. and 6:30 p.m. Fridays
at sunset and 9 a.m. on Saturdays
Cemetery: Tours by appointment
Insider Tip
Mikveh Israel follows the Spanish-Portuguese (Sephardic) ritual introduced by Reverend Gershom Mendez Seixas, who, in 1780, came to serve as Hazzan (Congregational Leader) .
Kids' Stuff
Phillip Moses Russell, surgeon's mate to General Washington, is buried in Mikveh Israel's cemetery.
44 N. Fourth Street
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In the neighborhood
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Philadelphia CityPASS
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- The Betsy Ross House
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Arts & Sciences Dean Mark Lawrence Kornbluh visited the Academic Science Building construction site to discuss its current progress and his excitement for what the facility means for the University of Kentucky. The Academic Science Building is scheduled to open Fall 2016.
credits: Reveal, University of Kentucky reveal.uky.edu/flynn_tj
Keith B. MacAdam was born in Rochester, N.Y., attended Swarthmore College and earned a doctorate in Physics in 1971. After research at University of Stirling in Scotland, Yale University, and the University of Arizona, he came to UK as an Assistant Professor in 1977. He built a campus-based research program in experimental atomic-molecular-optical (AMO) physics with students and post-docs, supported by the National Science Foundation and the Research Corporation. He was appointed Professor of Physics in 1986 and was a University Research Professor in 1990-91.
MacAdam's research in crossed-beam collisions between charged particles and laser-excited atoms in highly excited "Rydberg" states was widely recognized in the international AMO physics community. He was elected a Fellow of the American Physical Society in 1987. Research in Aarhus, Denmark, Boulder, Colo., and Stockholm, Sweden, extended his international connections.
At UK MacAdam was active in all aspects of teaching, research and service. He taught with success at levels from first-year to graduate, and he introduced and continues to teach a non-majors' physics course "How Things Work." He served as Chair of the Department of Physics & Astronomy (1997-2001), on the College Executive Committee (Chair, 2007-08) and on many campus-wide committees. MacAdam was honored by the naming of the UK MacAdam Student Observatory, which opened in 2008 to serve the campus and community.
Catch a sneak peek of the amazing opportunities with the College of Arts & Sciences!
Dr. Ravat's Exploring the Solar System class had the privilege of doing a Skype interview with NASA Astronaut Dr. Drew Feustel. The Mission Specialist veteran detailed his drive to become an astronaut, his experiences in Space, and how NASA research connects to life on Earth.
Watch the full video here! vimeo.com/63330398
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Swan Necklace & Ring made with Swarovski Elements£12 instead of £60 (from Cian Jewels) for a matching swan necklace and ring made with Swarovski Elements - save 80%
Save 80%
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- Get a swan necklace and ring.
- Made with Swarovski Elements.
- White gold-plated setting.
- With black or white detailing.
- Ring comes in XS, S, M or L.
- Gorgeous gift for a loved one.
The Fine Print
- May buy multiples.
- Voucher valid until 29th Jul 2014.
- Postage costs an additional £3.99 per voucher.
- Please allow up to 10 working days for delivery after redeeming.
- Subject to availability.
Full Details
There once was an ugly duckling with feathers all stubby and brown. But once today's deal swanned into sight, we didn't care...
We're offering you a matching swan necklace and ring made with Swarovski Elements for just £12, saving you 80% off Cian Jewels' price of £60 (correct as of 11.7.14).
Comprising a matching necklace and ring, this swan set is white gold plated and bejewelled with a Swarovski Elements crystal, carefully cut to reflect the light and exude elegance. The swan necklace hangs on a delicate chain, which is beautifully offset by the crystal detailing on the pendant. Whether you choose a black or white swan set, you'll be no ugly duckling wearing these jewels…
How to Redeem
- When the deal ends we'll send you your unique wowcher code.
- Head to the Wowcher redemption page using the link we send you.
- Enter your wowcher code and delivery details.
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The deal can also entitle you to a Wowcher Wallet Credit. Click here for more info.
Level 2 Emergency First Aid Class £29 instead of £75 for a day long, Level 2 emergency first aid workshop at CTC Supplies, Wolverhampton or Birmingham - save 61%
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Residence Inn Atlanta Gwinnett Place
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Travel Blogs from Duluth
On My Way!
While listening to my most favorite song at the moment (Turtle, by Alana Davis), I've realized my trip has BEGUN!!!! It wasn't real until now. I'm sitting at my seat on my way to JFK airport, waiting for my dinner, and I don't really have much to say except that my excitement is finally here. No worrying, no doubting, just excitement. That's all for now. Sorry if you found this post boring, but sometimes you just gotta gloat. Love you ...
The S&D Awards
... go stand in line. Little things (well, big things to us) like that go a long way. We really hated leaving there.
2nd Place: The Andaz Hotel in Tokyo. We had some lovely service here, too. The lady we first worked with, Andrea from India, was so knowledgeable and helped us with so many questions and details.
1st Place: The prettiest bed goes to the king sized bed at the Intercontinental Hotel in Hong Kong. It wasn't the most ...
... I last sore Linda well over 30 years or more. We had a great evening, great food and it was a shame we could not of stayed longer but we had already made plans to in North Carolina.
Atlanta-Tour End-Honolulu
... beer was awesome and we gave each other the biggest fist pump! We ate some yogurt covered pretzels and nibbles. I ordered us a grilled cheese sandwich each. It cost $7.99 each as they don't provide food like at home. It was really nice, with tomato and bacon, funny how they advertise it as only a grilled cheese.. Eventually after further delays, we boarded. It's now 3.10 pm and we have a long flight but we made it! Boarding this flight is absolute bedlam and craziness. Everyone ...
This blog place wants me to write a blurb for every place I went to, I guess it's to better map Martha's adventures. Sure, details about Atlanta! Here I met up with my University of Minnesota friends. Understanding the likelihood that the only eyes reading this are those of my parents', I will explain this nice and clear. Hi Mom. We've gone over this a couple of times, but these are my friend(s): Mary Ann: one of ...


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Jump to navigation
A U medical team delivers precision stimulation to the brain, relieving the debilitating symptoms of disorders like Parkinson's disease.
2 months ago
The University of Minnesota's Board of Regents will review and act on initial plans, including schematic designs and financing, for the U of M's Athletics Village project next week.
2 days ago
Department of Food Science and Nutrition
Morrine Omolo is committed to mentoring young girls, especially those interested in science, technology, engineering, and math education (STEM) fields.
3 months ago
Forty years ago peregrine falcons were at a low point worldwide — their eggshells thinned by the pesticide DDT. Today, the fierce raptors are an emblem for successful wildlife reintroductions. Their dramatic turnaround is highlighted in the newest exhibition at the University of Minnesota's Bell Museum.
3 days ago
The Minnesota Medical Foundation
Mathematicians have emerged as some of medicine's greatest unsung heroes, shedding light on complex biological processes, all to improve patient health.
A diverse coalition of public and private colleges and universities, including Carleton College, University of Minnesota and St. Olaf College, is coming together with the goal of improving the college admission application process for all students. The Coalition is developing a free platform of online tools to streamline the experience of planning for and applying to college.
The Economist
Post Bulletin
Star Tribune


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By: patxi1980
This is my top 10 list of the best adventure games created by LucasArts. Enjoy it! PD: The first game (laberynth) has no sound, but the others have sound.
Views: 859 | Posted: 01/20/2010
By: X-BoSS
12 Adventures in 24 hours!
Views: 293 | Posted: 06/01/2009
By: James
Here is the first episode of my series where i look at classic video games and what exactly makes them so special. Episode #1: Indiana Jones and the Fate of Atlantis
Views: 2,914 | Posted: 08/25/2008


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Two tiny objects with equal charges of 81.0 �C are placed at two corners of a square with sides of 0.305 m, as shown. How far above and to the left of the corner of the square labeled A would you place a third small object with the same charge so that the electric field is zero at A?
above corner A? ___________m
to the left of corner A?_________m


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Article | June 30, 1995
Field data recorder
Lansmont's (Monterey, CA) Shock and Vibration Environment Recorder measures drop heights, impacts, vehicle motion, temperature and humidity during transportation and distribution.
Can pinpoint damage assess vehicle and package performance and characterize the transport environment.
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text: Lee Gruenfeld Lee Gruenfeld is the bestselling author of novels such as The Halls of Justice and All Fall Down as well as the nonfiction Confessions of a Master Jewel Thief. He lives in Southern California. Become a Fan × BECOME A FAN Get updates about Lee Gruenfeld and recommended reads from Simon & Schuster. Plus, get a FREE eBook when you sign up! Email Address Free eBook available to NEW subscribers only. Offer redeemable at Bookshout.com. Code expires 12/31/2015. See full terms and conditions. By clicking SIGN UP, I acknowledge that I have read and agreed to the Privacy Policy and the Terms of Use. Books by this Author Becoming Holyfield By Evander Holyfield By Evander Holyfield Becoming Holyfield History's only four-time world heavyweight boxing champion and one of America's most admired and beloved athletes reveals the dramatic story of his rise from poverty to the very pinnacle of the toughest sport on earth. Barely able to make it into the heavyweight division and almost always the smaller fighter in the ring, Holyfield spent his professional career proving the naysayers wrong. Along the way he provided some of the twentieth century's most thrilling sports moments, not all of them on... Read more about this book Read more Get a FREE eBook when you join our mailing list. Plus, receive updates about Lee Gruenfeld,recommended reads and more from Simon & Schuster. Email Address Free eBook available to NEW subscribers only. Offer redeemable at Bookshout.com. Code expires 12/31/2015.See full terms and conditions and this month's choices.


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Top Teachers: Annette Hodges
Donahoe E.S., fifth grade
As a student teacher in inner-city Philadelphia, one of Annette Hodges' first assignments was to work with a Spanish-speaking youngster named Oscar.
"They sent him to me and said, 'If you can just teach him a couple words of English, great.' And I thought, Why only a couple?"
So for the next five months, Hodges worked with Oscar and two other students diligently – setting the same high expectations for them that she had experienced herself in private school as a child. By the end of the school year, Oscar was reading, spelling words correctly and earning A's on tests.
"He wanted it because he was excited that someone believed he could do it," she recalled.
That philosophy has guided Hodges through her 21 years as a teacher, including the past three at Donahoe Elementary in Sandston, where this year she is teaching a boys-only class of 17 fifth-graders.
The gender-specific class was Hodges' idea – something she said grew from her examination of single-sex private academies in which students seemed to excel.
"I thought, Why is is that that's not something that is offered in the public school setting?" she said. "I wanted to encourage [students] and give them expectations."
Hodges is drilling into her students' minds that they will become 'men of honor' by the end of the school year.
Every Monday, the boys "dress for success" by wearing khakis or blue jeans and a light blue Izod shirt that Hodges purchased each of them in September. The point? To teach them what success looks like and help them internalize it.
"Many of them did not know what success was," Hodges said, recalling a recent exercise in which one student said his mother was successful because she went to work every day, even though she hated her job. "They have to see it before they can truly believe it because they know what they're looking at. It's about breaking habits that they've seen or thought were okay and rebuilding things for them."
Her approach is working.
"Through tears, attitude, and smiles of the boys, the joy of accomplishment is the prize," one nominator wrote. "Ms. Hodges has a heart of a lion and patience of an angel. The spirit and success of the boys are the amazing result."
Though her students have scored well in reading and math tests, she is motivating them to achieve 100 percent passage rates in both.
"I just don't want them just simply to pass to say they passed," she said. "I want them to leave being able to transfer the knowledge to the following year. I want them to truly be true learners."
"I go to bed a great deal at night with a heavy heart because I'm thinking, 'How can I reach this one, how can I get that one to understand?' You don't have time to spend time celebrating successes."
HandsOn Day set for Oct. 17
Citizen Staff Reports 10/12/2015
HandsOn Day 2015, which will feature 1200 volunteers serving more than 65 nonprofits in Greater Richmond, will take place Oct. 17 from 9 a.m. to 1 p.m. Among the projects in Henrico or surrounding communities that need volunteers are: installing GardenFest lights Lewis Ginter Botanical Garden, distribution center assistance at Feedmore, pumpkin carving with residents at SupportOne, work and play at Housing Families First, paint for independence at Heart Havens, spruce up the shelter and clean sweep at Harbor House at Safe Harbor, pinwheel project at REAP and Kidney Walk prep at National Kidney Foundation Serving VA. > Read more.
Online tool helps identify best native plants for pollinators
Citizen Staff Reports 10/12/2015
Virginians who want to plant beneficial plants for bees, butterflies and hummingbirds have a new resource at their fingertips. The online Virginia Native Plant Finder now lets users search for native plant species that benefit pollinators. The tool, which is managed by the Virginia Natural Heritage Program, is free and easy to use; searches can be completed on desktops, tablets or smartphones.
Native plants are those that grow where they evolved; they have traits that enable them to adapt to local conditions. The Virginia Native Plant Finder lets users create their own custom native plant lists by selecting from dropdown menus. > Read more.
The Volunteers of the United States Army Field Band to perform Oct. 15
Henrico County Recreation and Parks is hosting a concert by The Volunteers of the United States Army Field Band on Thursday, Oct. 15 at Henrico Theatre, 305 East Nine Mile Road in Highland Springs. This free concert will be held at 7 p.m. and will feature Soldier-Musicians from the U.S. Army Field Band.
Since its inception in 1981, The Volunteers has been telling the Army story through rock, pop, country, and patriotic music. Its members have performed for millions of listeners in all 50 states, Puerto Rico, Canada, Mexico, Kuwait, and Iraq. This group tours more than 100 days each year, bringing a powerful message of patriotism and support to communities large and small. > Read more.
'World's Largest Truck Convoy' to travel through Henrico Saturday
Governor proclaims Oct. 15 Earthquake ShakeOut Day
Academy at Virginia Randolph to celebrate school's centennial Oct. 17
Business in brief
Henrico Master Gardener training program accepting applications
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Print Edition
Oct. 1, 2015Click here to read the print edition.
The Volunteers of the United States Army Field Band to perform Oct. 15
Weekend Top 10
New restaurant opens at Virginia Center Commons
Restaurant watch
Elliott Yamin to perform Friday at The Tin Pan
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Obituary – Mark Robert Reeves
Obituary – Hardin T. Brotherton
Obituary – John Patrick Markey
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HandsOn Day set for Oct. 17
Online tool helps identify best native plants for pollinators
More cyclists on the way
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The greatest love of all
My daughters' other dads
All hail to mothers and the hardest job of all
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Athlete of the Week – July 3: Kayla Sell
Athlete of the Week – June 19: Sydney Whitaker
Steward School's Enright drafted by NY Mets
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ClassifiedsWrap up your Holiday Shopping with 100 percent guaranteed, delivered-to-the-door Omaha Steaks! SAVE 68 percent PLUS 2 FREE GIFTS - 26 Gourmet Favorites ONLY $49.99. ORDER Today 1-888-691-0979 use code… Full text
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CalendarThe Short Pump Ruritan Club is seeking crafters for its 25th annual Short Pump Ruritan Craft Show on October 17 at Short Pump Middle School, 4701 Pouncey Tract Rd. For… Full text


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The food achieves the elusive goal of being honest, exciting, and bracingly creative while always remaining as delicious on the tongue as it is thrilling to the intellect.
Korean favorites like bibimbob and bulgogi repose on the menu alongside more Japanese-style dishes like salmon teriyaki.
The wicked favorite by pastry chef Thomas McCarthy will make your heart sing—and no, not just 'cause of the hemp seeds he's so craftily utilizing.
It's like an expensive designer Crunch, except the milk chocolate's crispy notes come from tiny bacon pieces and sweet maple, tinged with smoked sea salt.
It's light, creamy, plus swimming with fresh-shucked corn and huge, tender blue crab beignets smack-dab in the bowl's center.


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Gates: Troops could go to Pakistan
U.S. Secretary of Defense Robert Gates said Thursday that the United States is "ready, able and willing" to send troops to Pakistan if the government of the South Asian nation is interested.
U.S. military officials have told CNN that commanders are reviewing a classified planning order that could result in troops going to Pakistan for training purposes if Pakistan's government
The cost of any new training program would be in addition to the $750 million in security and economic aide the United States provides to the country.
Webmaster's Commentary:
It is only a matter of time before the $750 million dollar aid program will become inextricably linked to American military presence inside Pakistan.


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StartUpHire is the place to find Application Consultant (ERP) jobs. Review the list of Application Consultant (ERP) career opportunities to the left. These Application Consultant (ERP) jobs represent some of the most rewarding careers available.
You can refine your job search for Application Consultant (ERP) career opportunities by adding a job title, company name, or keyword to the search box above … or click the "Advanced Search" link for more options.
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Harris ERP is seeking talented, motivated individuals to install our innovative solutions for local government. We are looking for quality candidates to join our Professional Services team implementing our Payroll/HR and Financials solutions. You will have the opportunity to work8/30/2015 Broomfield, CO Save This Job
Job Description: The Technical Consultant shall be a motivated individual willing to leverage his or her experience and to adapt new skills as required to support various Services groups within Silvon. The responsibilities of the Technical Consultant shall include:9/8/2015 Chicago, IL Save This Job
Responsibilities: Compusearch Software Systems Inc., a leading provider of COTS-based procurement and grants management solutions for the federal government, is seeking technical consultants to support the design, development, implementation and operation of its solutions8/18/2015 Dulles, VA Save This Job
If you share a true passion for technology and enjoy working together to develop solutions, share ideas, and exchange knowledge we want to talk to you. Sparta Systems is the Leader in Enterprise Quality Management Solutions (EQMS) and moving forward! As a Delivery Consultant9/8/2015 Holmdel, NJ Save This Job
We continue to experience strong growth and are currently hiring Microsoft AX Support Analysts proficient in Microsoft Dynamics AX for our beautiful Denver office in the Tech Center. Microsoft AX Support Analysts provide support to our clients who are live with our solutions.9/15/2015 Denver, CO Save This Job
Boulder, CO LogRhythm is an industry leader in the fast-growing log management and security information and event management (SIEM) market. Our patented, award-winning security intelligence software platform helps Global 2000 companies, government agencies and mid-sized8/30/2015 Boulder, CO Save This Job
The Channels Solutions Consultant at MuleSoft is a seasoned pre-sales professional and Enterprise Architect in the integration/middleware domain, who understands the big picture - including the overall IT landscape and emerging technologies in the space.  The Channels9/17/2015 San Francisco, CA Save This Job
The Pre-sales team is seeking an Architect who can drive growth through API-led connectivity across the North America Channels business. Doing so requires a laser focus on empowering and enabling Channel Partners to deliver game changing connectivity driven solutions to their8/23/2015 San Francisco, CA Save This Job
Job responsibilities: The Solutions Consultant is a motivated, self-starter who is a key member of the Performix Solutions team. In this role, you will assist in requirements gathering, business and technical proposals, demonstration, design, and deployment of complex MES9/1/2015 Houston, TX Save This Job
Requisition Number15-0072 Post Date8/17/2015 TitleBusiness Analyst CityAlpharetta DescriptionBusiness Analyst Qualified candidate will have experience managing all aspects of the project development life cycle involving crossfunctional teams focused on the delivery of cloud10/1/2015 Alpharetta, GA Save This Job
Requisition Number15-0072 Post Date8/17/2015 TitleBusiness Analyst CityAlpharetta DescriptionBusiness Analyst Qualified candidate will have experience managing all aspects of the project development life cycle involving crossfunctional teams focused on the delivery of cloud9/1/2015 Alpharetta, GA Save This Job
Description Summary: Works with the NORAM sales team, primarily supporting our customers and prospects with our Enterprise Software Protection & Entitlement Management Solutions. Responsible for owning and driving the technical aspect of the sales cycle ? typically managing8/23/2015 Northern California Save This Job


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StartUpHire is the place to find Machine Learning Internship jobs. Review the list of Machine Learning Internship career opportunities to the left. These Machine Learning Internship jobs represent some of the most rewarding careers available.
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This position is located in San Francisco, CA. NerdWallet is seeking software engineer interns who are passionate about working in a high-growth start-up environment where there are real opportunities to make a meaningful and positive impact on consumers? financial lives.9/7/2015 San Francisco, CA Save This Job
The Parking Data Scientist will be working directly with the CTO to support the company?s data collection and inference strategy. This is a role requiring considerable breadth of expertise and system level thinking. You will be examining the company?s data archive and interfacing8/8/2015 Foster City, CA Save This Job
Quantcast is at the forefront of ad tech and big data. We hire the best and brightest from the industry across the entire company and Recruiting is no exception. The University Recruiting Manager will be responsible for Quantcast's University R9/5/2015 San Francisco, CA Save This Job