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Publications (25)70.05 Total impact
Article: Ultrastructural investigation of microcalcification and the role of oxygen–glucose deprivation in cultured rat hippocampal slices[Show abstract] [Hide abstract]
ABSTRACT: Intracellular calcium accumulation is associated with cell death in several neuropathological disorders including brain ischemia, but the exact mechanisms of calcification need to be clarified. We used organotypic hippocampal slice culture cultures subjected to oxygen-glucose deprivation (OGD) mimicking the in vivo situation to investigate the events underlying ectopic calcification. Alizarin red staining indicating calcium deposition was observed in the cornu ammonis (CA)1 and dentate gyrus regions in control hippocampal slices despite no specific labeling for cell death markers. Electron microscopy using the osmium/potassium dichromate method revealed scattered degenerated cells throughout the normally appearing CA1 region. They contained electron-dense precipitates within mitochondria, and electron probe microanalysis confirmed that they were calcifying mitochondria. Selective calcium deposition was noted within, but not beyond, mitochondria in these mineralized cells. They showed ultrastructural features of non-necrotic, non-apoptotic cell death and retained their compact ultrastructure, even after the majority of mitochondria were calcified. Unexpectedly, no intracellular calcification was noted in necrotic CA1 pyramidal cells after OGD, and there was no progression of calcification in OGD-lesioned slices. In addition, mineralized cells in both control and OGD-lesioned slices were closely associated with or completely engulfed by astrocytes but not microglia. These astrocytes were laden with heterogeneous cytoplasmic inclusions that appeared to be related with their phagocytic activity. These data demonstrate that microcalcification specifically associated with mitochondria might lead to a novel type of cell death and suggest that astrocytes may be involved in the phagocytosis of these mineralized cells and possibly in the regulation of ectopic calcification. Copyright © 2015 Elsevier B.V. All rights reserved.Brain research 07/2015; 1622. DOI:10.1016/j.brainres.2015.06.048 · 2.84 Impact Factor
Article: Differential expression of the calcium-sensing receptor in the ischemic and border zones after transient focal cerebral ischemia in rats[Show abstract] [Hide abstract]
ABSTRACT: G-protein-coupled calcium-sensing receptor (CaSR) has been recently recognized as an important modulator of diverse cellular functions, beyond the regulation of systemic calcium homeostasis. To identify whether CaSR is involved in the pathophysiology of stroke, we studied the spatiotemporal regulation of CaSR protein expression in rats undergoing transient focal cerebral ischemia, which was induced by middle cerebral artery occlusion. We observed very weak or negligible immunoreactivity for CaSR in the striatum of sham-operated rats, as well as in the contralateral striatum of ischemic rats after reperfusion. However, CaSR expression was induced in the ischemic and border zones of the lesion in ischemic rats. Six hours post-reperfusion there was an upregulation of CaSR in the ischemic zone, which seemed to decrease after seven days. This upregulation preferentially affected some neurons and cells associated with blood vessels, particularly endothelial cells and pericytes. In contrast, CaSR expression in the peri-infarct region was prominent three days after reperfusion, and with the exception of some neurons, it was mostly located in reactive astrocytes, up to day 14 after ischemia. On the other hand, activated microglia/macrophages in both the ischemic and border zones were devoid of specific labeling for CaSR at any time point after reperfusion, despite their massive infiltration in both regions. Our results show heterogeneity in CaSR-positive cells within the ischemic and border zones, suggesting that CaSR expression is regulated in response to the altered extracellular ionic environment caused by ischemic injury. Thus, CaSR may have a multifunctional role in the pathophysiology of ischemic stroke, possibly in vascular remodeling and astrogliosis. Copyright © 2015. Published by Elsevier B.V.Journal of chemical neuroanatomy 05/2015; 66-67. DOI:10.1016/j.jchemneu.2015.05.001 · 1.50 Impact Factor
Article: Expression of Vascular Endothelial Growth Factor-C (VEGF-C) and Its Receptor (VEGFR-3) in the Glial Reaction Elicited by Human Mesenchymal Stem Cell Engraftment in the Normal Rat Brain[Show abstract] [Hide abstract]
ABSTRACT: To determine whether vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) are involved in the glial reaction elicited by transplanted mesenchymal stem cells (MSCs), we examined the cellular localization of VEGF-C and VEGFR-3 proteins in the striatum of adult normal rats that received bone marrow-derived human MSCs. The MSC grafts were infiltrated with activated microglia/macrophages and astrocytes over a 2-week period post-transplantation, which appeared to parallel the loss of transplanted MSCs. VEGF-C/VEGFR-3 was expressed in activated microglia/macrophages recruited to the graft site, where the induction of VEGF-C protein was rather late compared with that of its receptor. VEGF-C protein was absent or very weak on day 3, whereas VEGFR-3 immunoreactivity was evident within the first three days. Furthermore, within three days, VEGF-C could be detected in the brain macrophages localized immediately adjacent to the needle track. At the same time, almost all the brain macrophages in both regions expressed VEGFR-3. Reactive astrocytes at the graft site expressed VEGFR-3, but not VEGF-C. These data demonstrated the characteristic time- and cell-dependent expression patterns for VEGF-C and VEGFR-3 within the engrafted brain tissue, suggesting that they may contribute to neuroinflammation in MSC transplantation, possibly through the recruitment and/or activation of microglia/macrophages and astrogliosis. © The Author(s) 2014.Journal of Histochemistry and Cytochemistry 12/2014; DOI:10.1369/0022155414564218 · 1.96 Impact Factor
Article: The Indolinone MAZ51 Induces Cell Rounding and G2/M Cell Cycle Arrest in Glioma Cells without the Inhibition of VEGFR-3 Phosphorylation: Involvement of the RhoA and Akt/GSK3 beta Signaling Pathways[Show abstract] [Hide abstract]
ABSTRACT: MAZ51 is an indolinone-based molecule originally synthesized as a selective inhibitor of vascular endothelial growth factor receptor (VEGFR)-3 tyrosine kinase. This study shows that exposure of two glioma cell lines, rat C6 and human U251MG, to MAZ51 caused dramatic shape changes, including the retraction of cellular protrusions and cell rounding. These changes were caused by the clustering and aggregation of actin filaments and microtubules. MAZ51 also induced G2/M phase cell cycle arrest. This led to an inhibition of cellular proliferation, without triggering significant cell death. These alterations induced by MAZ51 occurred with similar dose- and time-dependent patterns. Treatment of glioma cells with MAZ51 resulted in increased levels of phosphorylated GSK3β through the activation of Akt, as well as increased levels of active RhoA. Interestingly, MAZ51 did not affect the morphology and cell cycle patterns of rat primary cortical astrocytes, suggesting it selectively targeted transformed cells. Immunoprecipitation-western blot analyses indicated that MAZ51 did not decrease, but rather increased, tyrosine phosphorylation of VEGFR-3. To confirm this unanticipated result, several additional experiments were conducted. Enhancing VEGFR-3 phosphorylation by treatment of glioma cells with VEGF-C affected neither cytoskeleton arrangements nor cell cycle patterns. In addition, the knockdown of VEGFR-3 in glioma cells did not cause morphological or cytoskeletal alterations. Furthermore, treatment of VEGFR-3-silenced cells with MAZ51 caused the same alterations of cell shape and cytoskeletal arrangements as that observed in control cells. These data indicate that MAZ51 causes cytoskeletal alterations and G2/M cell cycle arrest in glioma cells. These effects are mediated through phosphorylation of Akt/GSK3β and activation of RhoA. The anti-proliferative activity of MAZ51 does not require the inhibition of VEGFR-3 phosphorylation, suggesting that it is a potential candidate for further clinical investigation for treatment of gliomas, although the precise mechanism(s) underlying its effects remain to be determined.PLoS ONE 09/2014; 9(9):e109055. DOI:10.1371/journal.pone.0109055 · 3.23 Impact Factor
Article: Induction of Kruppel-like factor 4 expression in reactive astrocytes following ischemic injury in vitro and in vivo. Histochem Cell Biol[Show abstract] [Hide abstract]
ABSTRACT: Krüppel-like factor 4 (KLF4) is a transcription factor with diverse and cell type-specific functions and is associated with a variety of pathophysiological processes. Recently, it has been proposed that the regulation of KLF4 is critical to neuronal differentiation and that neural progenitors overexpressing KLF4 take on a glial identity. The present study aimed to determine whether KLF4 is involved in the astroglial reaction induced by ischemia-reperfusion injury in the brain. No specific KLF4 immunoreactivity was observed in resting astrocytes of the control hippocampus, but significant induction was detected in reactive astrocytes preferentially located in the CA1 and dentate hilar regions of the hippocampus following transient forebrain ischemia. Astroglial KLF4 expression was induced in the nuclei and cytoplasm within 3 days of ischemia and persisted for at least 4 weeks. This pattern was reproduced in an in vitro astrogliosis model of rat primary cortical astrocytes exposed to oxygen-glucose deprivation (OGD). Furthermore, immunoblot assay showed that nuclear and cytosolic extracts from cortical astrocytes subjected to OGD had significantly higher levels of KLF4 protein compared to normoxic extracts. Thus, our data demonstrate that KLF4 expression was induced in astroglia by ischemic injury both in vivo and in vitro, suggesting that KLF4 may act as a transcription factor linked to the regulation of the astroglial reaction following ischemic injury.Histochemie 08/2013; 141(1). DOI:10.1007/s00418-013-1134-5 · 3.05 Impact Factor
Article: Characterization of nestin expression in astrocytes in the rat hippocampal CA1 region following transient forebrain ischemia[Show abstract] [Hide abstract]
ABSTRACT: Recent studies have suggested that nestin facilitates cellular structural remodeling in vasculature-associated cells in response to ischemic injury. The current study was designed to investigate the potential role of post-ischemic nestin expression in parenchymal astrocytes. With this aim, we characterized ischemia-induced nestin expression in the CA1 hippocampal region, an area that undergoes a delayed neuronal death, followed by a lack of neuronal generation after transient forebrain ischemia. Virtually all of the nestin-positive cells in the ischemic CA1 hippocampus were reactive astrocytes. However, induction of nestin expression did not correlate simply with astrogliosis, but rather showed characteristic time- and strata-dependent expression patterns. Nestin induction in astrocytes of the pyramidal cell layer was rapid and transient, while a long-lasting induction of nestin was observed in astrocytes located in the CA1 dendritic subfields, such as the stratum oriens and radiatum, until at least day 28 after ischemia. There was no detectable expression in the stratum lacunosum moleculare despite the evident astroglial reaction. Almost all of the nestin-positive cells also expressed a transcription factor for neural/glial progenitors, i.e., Sox-2 or Sox-9, and some cells were also positive for Ki-67. However, all of the nestin-positive astrocytes expressed the calcium-binding protein S100β, which is known to be expressed in a distinct, post-mitotic astrocyte population. Thus, our data indicate that in the ischemic CA1 hippocampus, nestin expression was induced in astroglia that were becoming reactive, but not in a progenitor/stem cell population, suggesting that nestin may allow for the structural remodeling of these cells in response to ischemic injury.Anatomy & cell biology 06/2013; 46(2):131-40. DOI:10.5115/acb.2013.46.2.131
Article: Characterization of nestin expression and vessel association in the ischemic core following focal cerebral ischemia in rats[Show abstract] [Hide abstract]
ABSTRACT: The present study aimed to provide a detailed characterization of the cellular phenotypes of nestin-positive cells in a rat model of ischemic stroke. Nestin-positive cells included reactive astrocytes in the peri-infarct region. In the ischemic core, in which astrocytes had virtually disappeared, nestin expression was exclusively associated with the vasculature, including the microvasculature and larger caliber vessels. Induction of nestin expression in the ischemic core occurred by 3 days post-ischemia. Nestin expression continued through at least 28 days post-ischemia but the cellular profiles of nestin-positive cells changed over this period. In the ischemic core at day 3, nestin-positive cells frequently had long processes that ran parallel along the longitudinal axis of the vasculature. These cells were highly proliferative and expressed the transcription factor for neural/glial progenitors, Sox9. Based on their morphological characteristics and on a double-labeling study, most nestin-positive cells were clearly distinguishable from vasculature-associated cells including endothelial cells, smooth muscle cells and microglia/macrophages. Immunoelectron microscopic findings demonstrated that most nestin-positive cells lay in the perivascular space and had macrophage-like features, indicating morphological similarity to perivascular macrophages. Nestin expression was still associated with the vasculature 14 days after ischemia but appeared in fibroblast-like cells. Thus, our data indicated that, in the ischemic core, nestin expression was not limited to a progenitor/stem cell population but was induced in the vasculature-associated cells. These cell types included perivascular macrophages and fibroblast-like cells that appeared to undergo dynamic structural changes. These results suggest that nestin facilitates cellular structural remodeling in response to ischemic injury.Cell and Tissue Research 12/2012; 351(3). DOI:10.1007/s00441-012-1538-x · 3.57 Impact Factor
Article: Upregulation of Vascular Endothelial Growth Factor Receptor-3 in the Spinal Cord of Lewis Rats with Experimental Autoimmune Encephalomyelitis[Show abstract] [Hide abstract]
ABSTRACT: We investigated the spatiotemporal expression of vascular endothelial growth factor receptor-3 (VEGFR-3) in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. VEGFR-3 mRNA and protein were constitutively expressed in gray matter neurons and in a few white matter astrocytes. Induction of VEGFR-3 occurred predominantly in perivascular infiltrated macrophages in the spinal cord white matter during the inductive phase of EAE. VEGFR-3 expression was also induced in activated microglial cells in the gray and white matter, mainly in the peak phase. In addition, reactive astrocytes in the white matter, but not in the gray matter, expressed VEGFR-3 as disease severity increased. These data suggest that VEGFR-3 is involved in the recruitment of monocytic macrophages and in glial reactions during EAE.Journal of Histochemistry and Cytochemistry 09/2012; 61(1). DOI:10.1369/0022155412462975 · 1.96 Impact Factor
Article: Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats[Show abstract] [Hide abstract]
ABSTRACT: Vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, has recently been reported to be induced within vessel-like structures in the ischemic brain. The purpose of the present study was to characterize and define further the cellular phenotypes of vascular-associated cells that manifest induced VEGFR-3 expression in a rat model of ischemic stroke. Vessel-associated cells expressing VEGFR-3 were found to be perivascular astrocytes in the peri-infarct region, whereas in the ischemic core, where astrocytes had virtually disappeared, induction of VEGFR-3 mRNA and protein was still prominent in vascular structures 3-7 days after reperfusion. VEGFR-3 and nestin expression were colocated in almost all cells associated with the vasculature in the ischemic core, and most (∼82%) of the VEGFR-3/nestin double-labeled cells were proliferative. A subpopulation of these VEGFR-3-expressing cells appeared to be included in two immunophenotypically distinct perivascular cells: NG2-positive pericytes and ED2- or OX6-perivascular macrophages. However, most of these cells did not show markers for vasculature-associated cell types such as endothelial cells, microglia/macrophages, and smooth muscle cells. Thus, our data indicated that vasculature-associated VEGFR-3-expressing cells in the ischemic core may represent a heterogeneous population of cells with functional diversity, rather than a uniform cell type.Acta histochemica 07/2012; 115(2). DOI:10.1016/j.acthis.2012.06.005 · 1.71 Impact Factor
Article: Sustained Expression of Osteopontin Is Closely Associated with Calcium Deposits in the Rat Hippocampus After Transient Forebrain Ischemia[Show abstract] [Hide abstract]
ABSTRACT: The present study was designed to evaluate the extent and topography of osteopontin (OPN) protein expression in the rat hippocampus 4 to 12 weeks following transient forebrain ischemia, and to compare OPN expression patterns with those of calcium deposits and astroglial and microglial reactions. Two patterns of OPN staining were recognized by light microscopy: 1) a diffuse pattern of tiny granular deposits throughout the CA1 region at 4 weeks after ischemia and 2) non-diffuse ovoid to round deposits, which formed conglomerates in the CA1 pyramidal cell layer over the chronic interval of 8 to 12 weeks. Immunogold-silver electron microscopy and electron probe microanalysis demonstrated that OPN deposits were indeed diverse types of calcium deposits, which were clearly delineated by profuse silver grains indicative of OPN expression. Intracellular OPN deposits were frequently observed within reactive astrocytes and neurons 4 weeks after ischemia but rarely at later times. By contrast, extracellular OPN deposits progressively increased in size and appeared to be gradually phagocytized by microglia or brain macrophages and some astrocytes over 8 to 12 weeks. These data indicate an interaction between OPN and calcium in the hippocampus in the chronic period after ischemia, suggesting that OPN binding to calcium deposits may be involved in scavenging mechanisms.Journal of Histochemistry and Cytochemistry 04/2012; 60(7):550-9. DOI:10.1369/0022155412441707 · 1.96 Impact Factor
Article: Overlapping Distribution of Osteopontin and Calcium in the Ischemic Core of Rat Brain after Transient Focal Ischemia[Show abstract] [Hide abstract]
ABSTRACT: Osteopontin (OPN), an adhesive glycoprotein, has recently been proposed to act as an opsonin that facilitates phagocytosis of neuronal debris by macrophages in the ischemic brain. The present study was designed to elucidate the process whereby OPN binds to neuronal cell debris in a rat model of ischemic stroke. Significant co-localization of the OPN protein and calcium deposits in the ischemic core were observed by combining alizarin red staining and OPN immunohistochemistry. In addition, electron microscopy (EM) using the osmium/potassium dichromate method revealed that electron-dense precipitates, typical of calcium deposits, were localized mainly along the periphery of putative degenerating neurites. This topical pattern of calcium precipitates resembled the distribution of OPN as detected by immunogold-silver EM. Combining immunogold-silver EM and electron probe microanalysis further demonstrated that the OPN protein was localized at the periphery of cell debris or degenerating neurites, corresponding with locally higher concentrations of calcium and phosphorus, and that the relative magnitude of OPN accumulation was comparable to that of calcium and phosphorus. These data suggest that calcium precipitation provides a matrix for the binding of the OPN protein within the debris or degenerating neurites induced by ischemic injury. Therefore, OPN binding to calcium deposits may be involved in phagocytosis of such debris, and may participate in the regulation of ectopic calcification in the ischemic brain.Journal of neurotrauma 11/2011; 29(7):1530-8. DOI:10.1089/neu.2011.2078 · 3.71 Impact Factor
Article: Distribution of vascular endothelial growth factor receptor-3/Flt4 mRNA in adult rat central nervous system[Show abstract] [Hide abstract]
ABSTRACT: Vascular endothelial growth factor receptor (VEGFR)-3/Flt4 binds VEGF-C and VEGF-D with high affinity. It has been suggested to be involved in neurogenesis and adult neuronal function. However, little is known about the localization of VEGFR-3 in the adult central nervous system (CNS). The present study presents, to our knowledge, the first detailed mapping of VEGFR-3 mRNA expression in adult rat brain and spinal cord by using in situ hybridization and reverse transcription-polymerase chain reaction analysis (RT-PCR). Varying VEGFR-3 expression intensity was detected in functionally diverse nuclei, with the highest levels in the mitral cells of the olfactory bulb, piriform cortex, anterodorsal thalamic nucleus, several nuclei of the hypothalamus, and the brainstem cranial nerve nuclei. VEGFR-3 mRNA was abundantly expressed in the ventral motor neurons of the spinal cord and in some circumventricular organs such as the median eminence and the area postrema. Moreover, the locus coeruleus and some of the nuclei of the reticular formation showed moderate-to-high hybridization signals. VEGFR-3 expression appeared to be localized mostly within neurons, but weak labeling was also found in some astrocytes. In particular, VEGFR-3 was highly expressed in ependymal cells of the ventral third ventricle and the median eminence, which were co-labeled with vimentin but not with glial fibrillary acidic protein, suggesting that these cells are tanycytes. RT-PCR analysis revealed similar levels of VEGFR-3 expression in all regions of the adult rat CNS. The specific but widespread distribution of VEGFR-3 mRNA in the adult rat CNS suggests that VEGFR-3 functions more broadly than expected, regulating adult neuronal function playing important roles in tanycyte function.Journal of chemical neuroanatomy 06/2011; 42(1):56-64. DOI:10.1016/j.jchemneu.2011.06.001 · 1.50 Impact Factor
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ABSTRACT: Osteopontin (OPN) is an adhesive glycoprotein linked to a variety of pathophysiological processes. We investigated whether OPN might act as an opsonin in the diseased brain by studying the postischemic expression and localization of OPN mRNA and protein in a rat model of ischemic stroke. In addition, we characterized the subcellular localization of OPN protein in the ischemic brain core. Induction of OPN mRNA occurred in activated microglia/macrophages in the ischemic core on days 3-7 after reperfusion and this was sustained up to day 28, at least. OPN protein was synthesized and secreted by brain macrophages, which first surrounded damaged striatal white matter tracts and then infiltrated into them. Punctate OPN-immunoreactive profiles were scattered throughout the infarction core except in white matter bundles. Electron microscopy showed the localization of OPN protein along the membranes lining what appeared to be the debris of dead neurons. These were located in the extracellular space and within the cytoplasm of brain macrophages, indicating that the OPN protein accumulated selectively on the surface of dead cells, most of which were phagocytosed subsequently by brain macrophages. However, no significant induction of OPN occurred in degenerating striatal white matter tracts or in brain macrophage-engulfed axonic or myelin debris. These data suggest that OPN secreted by brain macrophages in this rat model of stroke might be involved in the phagocytosis of fragmented cell debris and possibly not in the phagocytosis of axonic or myelin debris.Glia 03/2011; 59(3):413-23. DOI:10.1002/glia.21110 · 6.03 Impact Factor
Article: Immunochemical changes of calbindin, calretinin and SMI32 in ischemic retinas induced by increase of intraocular pressure and by middle cerebral artery occlusion[Show abstract] [Hide abstract]
ABSTRACT: The reaction of neuroactive substances to ischemic conditions in the rat retina evoked by different methods was immunochemically evaluated in adult Sprague-Dawley rats. Ocular ischemic conditions were unilaterally produced by elevating intraocular pressure (EIOP) or by middle cerebral artery occlusion (MCAO). Two EF-hand calcium binding proteins, calbindin D28K (CB) and calretinin (CR), in the normal retina showed similar immunolocalization, such as the amacrine and displaced amacrine cells, the ganglion cells, and their processes, particularly CB in horizontal cells. CB immunoreactive neurons in the ganglion cell layer in both types of ischemic retinas were more reduced in number than CR neurons compared to those in a normal retina. The CB protein level in both ischemic retinas was reduced to 60-80% of normal. The CR protein level in MCAO retinas was reduced to about 80% of normal but increased gradually to the normal value, whereas that in the EIOP showed a gradual reduction and a slight recovery. SMI32 immunoreactivity, which detects a dephosphorylated epitope of neurofilaments-M and -H, appeared in the axon bundles of ganglion cells in the innermost nerve fiber layer of normal retinas. The reactivity in the nerve fiber bundles appeared to only increase slightly in EIOP retinas, whereas a moderate increase occurred in MCAO retinas. The SMI32 protein level in MCAO retinas showed a gradual increasing tendency, whereas that in the EIOP showed a slight fluctuation. Interestingly, the MCAO retinas showed additional SMI32 immunoreactivity in the cell soma of presumed ganglion cells, whereas that of EIOP appeared in the Müller proximal radial fibers. Glial fibrillary acidic protein (GFAP) immunoreactivity appeared in the astrocytes located in the nerve fiber layer of normal retinas. Additional GFAP immunoreactivity appeared in the Müller glial fibers deep in EIOP retinas and at the proximal end in MCAO retinas. These findings suggest that the neurons in the ganglion cell layer undergo degenerative changes in response to ischemia, although EIOP retinas represented a remarkable Müller glial reaction, whereas MCAO retinas had only a small-scaled axonal transport disturbance.Anatomy & cell biology 03/2011; 44(1):25-34. DOI:10.5115/acb.2011.44.1.25
Article: Induction of vascular endothelial growth factor receptor-3 mRNA in glial cells following focal cerebral ischemia in rats[Show abstract] [Hide abstract]
ABSTRACT: To identify whether vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, is involved in pathophysiology of stroke, we investigated the spatiotemporal regulation of VEGFR-3 mRNA after transient focal cerebral ischemia. Most of the increase in VEGFR-3 expression in the ischemic core could be attributed to brain macrophages, whereas VEGFR-3 in the peri-infarct penumbra region was predominantly expressed in reactive astrocytes. A subpopulation of VEGFR-3-expressing brain macrophages was positive for NG2 proteoglycan and showed proliferative activity. In addition, in vitro model of stroke revealed no significant induction of VEGFR-3 in activated microglial cells, indicating that infiltrating exogenous macrophages expressed VEGFR-3 after focal ischemia. These data suggest that VEGFR-3 may be involved in the glial reaction and possibly in the recruitment of monocytic macrophages during ischemic insults.Journal of neuroimmunology 12/2010; 229(1-2):81-90. DOI:10.1016/j.jneuroim.2010.07.008 · 2.47 Impact Factor
Article: Expression of Vascular Endothelial Growth Factor Receptor-3 mRNA in the Developing Rat Cerebellum[Show abstract] [Hide abstract]
ABSTRACT: Vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, has recently been suggested to play an important role during neuronal development. To characterize its potential role in CNS ontogenesis, we investigated the spatiotemporal and cellular expression of VEGFR-3 in developing and mature rat cerebellum using in situ hybridization. VEGFR-3 expression appeared as early as E15, and was restricted to the ventricular zone of the cerebellar primordium, the germinative neuroepithelium, but was absent by E20. Instead, the expression area of VEGFR-3 in the cerebellum grew in parallel with cerebellar development. From E20 on, two populations of VEGFR-3-expressing cells can be clearly distinguished in the developing cerebellum: a population of differentiating and postmitotic neurons and the Bergmann glia. VEGFR-3 expression in neurons occurred during the period of neuronal differentiation, and increased with maturation. In particular, the expression of VEGFR-3 mRNA revealed different temporal patterns in different neuronal populations. Neurons generated early, Purkinje cells, and deep nuclear neurons expressed VEGFR-3 mRNA during late embryonic stages, whereas VEGFR-3 transcription in local interneurons appeared by P14 with weaker expression. In addition, Bergmann glia expressed VEGFR-3 throughout cerebellar maturation into adulthood. However, receptor expression was absent in the progenitors in the external granular layer and during further migration. The results of this study suggest that VEGFR-3 has even broader functions than previously thought, regulating both developmental processes and adult neuronal function in the cerebellum.Cellular and Molecular Neurobiology 11/2010; 31(1):7-16. DOI:10.1007/s10571-010-9530-z · 2.51 Impact Factor
Article: Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury[Show abstract] [Hide abstract]
ABSTRACT: The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.Neurochemical Research 10/2010; 35(10):1628-34. DOI:10.1007/s11064-010-0223-2 · 2.59 Impact Factor
Article: Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina[Show abstract] [Hide abstract]
ABSTRACT: Vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, is expressed in neural progenitor cells, but there has been no comprehensive study of its distribution in the developing brain. Here, the temporal and cell-specific expression of VEGFR-3 mRNA was studied in the developing rat forebrain and eye. Expression appeared along the ventricular and subventricular zones of the lateral and third ventricles showing ongoing neurogenesis as early as embryonic day 13 but was progressively down-regulated during development and remained in the subventricular zone and rostral migratory stream of the adult forebrain. VEGFR-3 expression was also detectable in some differentiating and postmitotic neurons in the developing cerebral cortex, including Cajal-Retzius cells, cortical plate neurons, and subplate neurons. Expression in the subplate increased significantly during the early postnatal period but was absent by postnatal day 14. It was also highly expressed in nonneural tissues of the eye during development, including the retinal pigment epithelium, the retinal ciliary margin, and the lens, but persisted in a subset of cells in the pigmented ciliary epithelium of the adult eye. In contrast, there was weak or undetectable expression in the early neural retina, but a subset of retinal neurons in the postnatal and mature retina showed intense signals. These unique spatiotemporal mRNA expression patterns suggest that VEGFR-3 might mediate the regulation of both neurogenesis and adult neuronal function in the rat forebrain and eye.The Journal of Comparative Neurology 04/2010; 518(7):1064-81. DOI:10.1002/cne.22263 · 3.23 Impact Factor
Article: Spatial and temporal changes of osteopontin in oxygen-glucose-deprived hippocampal slice cultures[Show abstract] [Hide abstract]
ABSTRACT: We have examined the temporal changes and cellular localization of osteopontin (OPN) mRNA and protein in organotypic hippocampal slice cultures subjected to ischemia-like oxygen-glucose deprivation (OGD). The sequential induction pattern response consisted of neuronal and microglial OPN upregulation, followed by a later extended phase of expression in reactive astrocytes. OPN immunoreactivity after OGD matched the mRNA induction patterns. Activated microglia revealed OPN staining in focal deposits, whereas neurons and reactive astrocytes showed perinuclear staining with a punctate cytosolic pattern of OPN, typical of secreted proteins. These data demonstrated that the temporal and cellular patterns of OPN induction in reactive glial cells in this in vitro model closely correlated with that in the in vivo model, suggesting that OPN has a multifunctional role in the pathogenesis of ischemic injury.Acta neurobiologiae experimentalis 01/2010; 70(1):1-12. · 1.29 Impact Factor
Article: Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats[Show abstract] [Hide abstract]
ABSTRACT: Vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, has recently been proposed to be involved in adult hippocampal neurogenesis in response to cerebral ischemia. To identify whether VEGFR-3 is involved in poststroke neurogenesis, we investigated the temporal regulation of VEGFR-3 mRNA expression in the subventricular zone (SVZ) of rats with transient focal cerebral ischemia by in situ hybridization analysis, and identified the phenotypes of cells expressing VEGFR-3 by double- and triple-labeling techniques. In sham-operated rats, hybridization signals for VEGFR-3 mRNA were evident at a weaker intensity in the SVZ of the lateral ventricle. VEGFR-3 was transiently increased in the dorsolateral SVZ of the infarcted hemisphere on days 3-7 after reperfusion. Almost all VEGFR-3-expressing cells in the ipsilateral SVZ were colabeled with glial fibrillary acidic protein and the neural progenitor marker nestin, and were highly proliferative. In addition, a subset of VEGFR-3-labeled cells in the ipsilateral SVZ expressed the immature neuronal marker, polysialic acid-neural cell adhesion molecule. These data indicate that VEGFR-3 is upregulated in SVZ astrocytes and immature neurons after focal ischemia, suggesting that VEGFR-3 might mediate the adult neurogenesis after ischemic stroke.Neuroscience Letters 12/2009; 469(2):194-8. DOI:10.1016/j.neulet.2009.11.073 · 2.03 Impact Factor
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Catholic University of Korea
Sŏul, Seoul, South Korea
- • Cell Death Disease Research Center
- • Department of Anatomy


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The Full Stack Software Engineer collaborates with a team to build engaging and effective shopping experiences for Etsy's community of buyers.9/17/2015 Brooklyn, NY Save This Job
The Senior Full Stack Software Engineer collaborates with a team to build engaging and effective shopping experiences for Etsy's community of buyers.9/17/2015 San Francisco, CA Save This Job
As a Product Branding Software Engineer you'll collaborate with our award-winning design team to build beautiful and engaging sites and experiences for a variety of audiences.8/29/2015 Brooklyn, NY Save This Job
As an engineer on Etsy's Payments team, you'll be responsible for the architecture and systems that process thousands of transactions on the Etsy platform every day.10/10/2015 Brooklyn, NY Save This Job
meQuilibrium offers a breakthrough, personalized stress management solution that helps people cope with today?s daily pressures, so they live happier, healthier and more productive lives. Our suite of digital and mobile apps are based on clinically validated research with proven9/15/2015 Boston, MA Save This Job
Care2.com is seeking an exceptional high level PHP engineer to positively impact the world around us on daily basis. The right candidate will have 7+ years of LAMP environment experience developing and maintaining complex websites and backend tools as well as a strong mid-level9/6/2015 Redwood City, CA Save This Job
Zend is looking for an experienced Senior Technical Support Engineer to join the Americas Global Services team. The Sr. Technical Support Analyst is responsible for daily communication with Zend?s customers, strategic partners and field operation teams. The Sr. Analyst oversees8/11/2015 Cupertino, CA Save This Job
The Linux Systems and Cloud Automation Engineer is responsible for finding innovative ways to further automate and improve resiliency and performance of our online product platform. TouchTunes infrastructure is composed mainly of Linux systems running Oracle, apache, Tomcat,9/5/2015 New York, NY Save This Job
Airliners has been the leader in aviation photography since 1995 with tens of millions of monthly visitors. The site is currently in the process of a major rebuild with the hottest technologies like ElasticSearch, Redis, PHP, Vagrant, Akamai CDN, Puppet and much more. Want to be10/8/2015 Santa Monica, CA Save This Job
Job Description At CloudFlare, we have our eyes set on an ambitious goal -- to build a better web. We believe that with our talented team, smart technology and engaged users we can solve some of the biggest problems on the web. We already serve more web traffic than Twitter,9/8/2015 San Francisco, CA Save This Job
meQuilibrium offers a breakthrough, personalized stress management solution that helps people cope with today?s daily pressures, so they live happier, healthier and more productive lives. Our suite of digital and mobile apps are based on clinically validated research with proven9/15/2015 Boston, MA Save This Job
Portland, Oregon Overview The Web Developer reports to the CTO and is responsible for supporting Customer Success, Marketing, Sales, and Product development. Successful candidates will be collaborative in approach but able to be productive independently. Must have good8/5/2015 Portland, OR Save This Job
DESCRIPTION We are looking for a Sales Engineer/Product Specialist to help support our growing business development team and customer pipeline. In addition the Sales Engineer will play an active role in contributing to the overall success of projects on behalf of our global7/18/2015 Palo Alto, CA Save This Job
RockYou, a leading social gaming company is seeking a Staff Software Engineer to be part of the engineering team and help design and build technology to be used across the organization. Responsibilities: Develop centralized technologies and tools for games and other9/16/2015 San Francisco, CA Save This Job
RockYou, a leading social gaming company, is seeking a Principal Software Engineer to be part of the engineering team and lead the migration of acquired games. Responsibilities: Manage the migration of games acquired from other companies to RockYou Investigate the tech stack9/16/2015 San Francisco, CA Save This Job

subset: commoncrawl


text:
Watch To koritsi me ta paramythia: A young girl, who lives in a poor neighborhood with her little brother, falls in love with a rich young man.
|80 mins
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Cute Flatroof Studio Central HKGHong KongHong Kong IslandHong Kong
About this listing
A great 300 sqf balcony in the heart of Hong Kong, to enjoy a quite glass of wine while over looking a park surrounded by big beautiful trees!!!!!!
This apartment is on the 1st floor and is in a very quite street in the heart of the old HKG.
Space where guests can cook their own meals
Towels, bed sheets, soap, and toilet paper
The Space
In Hong Kong a Beautiful Modern interior.
1st floor low rise in Sai Ying Pun with a extra Large Terrace 300 sqf area including a BBQ Girll.....
Amenities are :
Queen size bed w/ amazing mattress
Fully equipped kitchen with oven, microwave and cook top
Full size fridge / freezer
Unlimited high speed Wi-fi internet access included
New Flat screen TV with cable, USB ports and HDMI ports
Split type air-condition / heating
Iron with Ironing board
Ample closet space
Stylish wet room with rain shower
Fresh Towels changed every 3-4 days (depending on length of stay)
Fresh bed linen changed every 6-7 days
(depending on length of stay)
A pullout bed or mattress can be offered for extra guest
Guest Access
Direct access to all the main trams and buses allowing fast and convenient links to anywhere in the city.
Only 12 minutes walk to central or a 5 min taxi.
Now just 5mins away to the MTR Station Sai Ying Pun.
Interaction with Guests
Whole apartment will be for you during your stay.
You may enjoy a local home in heart of the old Hong Kong City, 5 min away from the famous LKF and Soho.
The Neighborhood
Getting Around
Once you got the hang of the trams and buses you could jump on one and with 10 minutes be at the Central MTR subway station and from there quickly go to all the main attractions of Hong Kong. (including the Peak/Tram, Sky Scrapers, Star ferry and mid-Level escalators) If you wanted some betting action there was a modern Hong Kong Jockey club right down the street where you could bet on just about anything and play the Hong Kong Lottery.
The main grocery store is a short walk but the 24 hour 7-11 is just around the corner with a full selection of basics + water, sim card and ice.... even alcohol!!!
A very efficient Chinese laundry around the corner. Small local restaurants are up and down on a neighboring street.
Other Things to Note
Best for 2 can do 3 using the sofa or 4 with a extra mattress added as well.
House Rules "PLEASE READ CAREFULLY"
Please note all bookings are for 2 guests automatically, the 3rd guest onwards is charged at US$20 per night, please ensure you have the correct number of guest listed when you book.
Check in time is AFTER 2:00 pm. Please ensure we have your flight details so we can arrange for your arrival. Please make sure to contact us by phone / text once you have collected your bags and we will arrange to be at the apartment within 45 min of that time so we can meet you at the flat to hand you the keys and acquaint you with the surroundings.
Check out time is at 12:00 Noon, and our cleaning crew will arrive very shortly after to service and inspect the flat for the next guest, if you would like to have a late check-out please ask via email and wait for confirmation.
UPON ARRIVAL AT PROPERTY, ALL GUESTS MUST WAIT FOR ME AT THE FRONT GATE OUTSIDE OF THE BUILDING. BUILDING RESIDENTS WILL COMPLAIN TO PROPERTY MANAGEMENT IF THE LOBBY AREA INSIDE BUILDING IS BLOCKED and not enter the building without us as this causes unnecessary misunderstandings with the property management and residents of the buildings. THANKS FOR YOUR KIND UNDERSTANDING FOR AN ENJOYABLE STAY.
Please KEEP NOISE GOING IN AND OUT THE FLAT TO A MINIMUM other residents often complain of my guest to noisy in the stair ways.
No Smoking!!!!!! This is a strictly NON-SMOKING Flat.
Please be careful of the mattress and sofas, stream cleaning is expensive in HK and we will charge if you are careless.
Please help us save the environment by turning off electrical appliances when leaving the apartment or are not using them. If you leave the A/C or heating and lights on carelessly we will charge for extra power. We charge US$10 per day. Water heater needs to be turned off when not using. If it breaks due to prolonged time left on, you will not have any hot water for your stay and will be subject to expensive repair charges.
There are dirty towels under the sink for cleaning spills and dirty (website hidden) NOT use the bath towels for cleaning spills if you do and they stain we will charge HK$80hk per sm towel and HK$120 per lg towel. This includes makeup stains.
For any damages or breakages we prefer if you contact me directly to discuss rather than we hold back you security deposit.... for example if there is a broken glass as it is quite a regular accurance we prefer if you leave cash on the counter to replace it a quick and easier solution for all involved.
In the case that you have an evening departure flight:
Please note that you can check in your luggage at any time during the same day as your flight at the Hong Kong Station Airport Express, which is only a 5 minute taxi or bus ride from the apartment. That way you would be free to explore the city on your last day, not having to worry about your luggage or rushing to the airport since you have already checked in, you will not have to be at the airport as early as everyone else.
All airlines flying from Hong Kong airport offer In Town check in, EXCEPT the following:
Air Philippine
Angel Air
Jetstar Asia Airlines
Orienal Thai
Pakistan International
53 Reviews
About the Host, Consuello
Great Fun Loving Traveller!!!!!!
I has been living in HK for the past 20 years and i love it!!!!!


text:
Transatlantic travel via ship
View Single Post
September 1st, 2012, 10:27 PM
Join Date: Aug 2011
Location: Ashburn, VA
Posts: 1,794
The best prices for transatlantic crossings are going to be repositioning cruises.
In the Spring, cruise lines move their ships from the Caribbean to Europe and they offering some very good deals.
Prices shown are per person and do not include taxes. A single will normally pay double the price. Now here's the catch; an advertise price consists of two fares - the cruise fare and the port fees. When traveling alone, some cruise lines will double the cruise fare but not the port fees. Some cruise lines, like NCL for example, will double the cruise fare and double the port fees. Just their way of making more profit because they do not need to double the port fees. (The ports charge these fees based on the number of people, not the number of cabins.)
As for finding another single to share a cabin, this use to be more prevalent than it is now, what with all the litigation problems. So it's always best to find someone to go with you or just bite the bullet and go by yourself.
But you'll definitely want to check out the transatlantic repositioning cruises as you'll find some good rates.
Travel Agent/Cruise Specialist w/13 yrs exp and 48 Cruises on 11 cruise lines!
Favorites: Paul Gauguin - Tahiti: Uniworld River Cruises - Europe; Celebrity Solstice-class ships; Holland America - 12-nights Baltics & Russia; RCCL - 14-nights Greek Isles, Turkey, & Croatia; Holland America - 14-day Alaskan cruisetour; 10-night Canada/New England cruise; 21 days Hawaii w/7-night NCL cruise; Oceania - 25 days in Asia; more than 3 months touring Europe by train. And many all-inclusive resorts!
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Now that we're deep into autumn and winter is growing closer, I'm starting to think more about my fatbike and riding on snow. Right now, I know of a few places that formally allow fatbike snow riding: Kingdom Trails, Millstone, and I think Trapps was dabbling with it last winter. Are there others?
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Knights Inn Cortez
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Learning about Ancestral Pueblo people
... way. Silverton is an old mining town that experienced rapid growth in the 1880s thanks to huge silver deposits being found. The free museum was really interesting and packed with a host of objects relating to the building and running of the railway.
A fair number of outdoors hunting and shooting shops caught our attention so another chance to see knives and guns!
Cortez, Mesa Verde, Durango and return - 136 miles
Starting the day at Mesa Verde
Native American flute music playing as we eat from a tasty breakfast buffet while looking out over some beautiful countryside. I definitely prefer this to the dry, splotchy green that has encompassed most of what we've seen since landing in Las Vegas. And GOOD-BYE miles and miles if straight roads!
After ...
From Our House to Theirs - Pueblo Peoples
... it humbling when I see these"
living simple" Pueblo people creating engineering feats (that we have just
improved on)and building structures that have lasted centuries. I cannot think
of anything built today that will be here even 1 century from now. That being
said I do enjoy my creature comforts.
Now for Balcony House. This is a ranger lead tour. We had to
walk down to just below the cliff house. Then we had to climb a 27 foot ladder
Cities under Cliffs
... ladder to get to the cliff palace. We viewed the round kivas and looked into some of the rooms before climbing out. We climbed stairs through rock outcroppings then three ladders surrounded by rocks to bet back to the top. Lunch was at a picnic table there. We then headed out, stopping at a couple of overlooks. We next went to the Spruce Tree House. There is a short, steep, paved trail down to this house. It is the ...
Mesa Verde
... old arrow-heads, tools, pottery, weavings (basketry) and way more - you could spend hours there. We didn't, as we wanted to see the ruins. So, the self-guided tour wasn't really – there were two rangers there; they just didn't lead you down the trail. It was Spruce Tree House, the best-preserved and 3rd largest cliff dwelling; they think it housed 60 to 80 people, and has 130 rooms and ...


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Lehigh Valley families are notoriously busy, with the region's hard-work ethic extending to kids in sports programs and parents at meetings alike. Keep it all straight with a stylish calendar, where you can also mark important dates like Dorney Park's opening day and IronPigs home games.
Don't sacrifice style when it comes to family calendars and message boards. In keeping with your Lehigh Valley home's style, add a vintage gold metal picture frames or eye-catching magnets. These can turn everyday notes and schedules into artwork.
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The everyday life of a busy family demands a family calendar – big, bold and impossible to overlook. Put it where everyone in your home is sure to see it, and make it a showstopper.
Eye-Catching Organization
A framed Plexiglas grid makes an easy annotated perpetual calendar. Each day of the month gets its own square and each member of the family has his or her own colored marker to make notes.
One Day At a Time
Use a simple magnetic strip to keep track of the ephemera – from tickets and reminders to post-its and letters – associated with each day of the week.


text:
Eastglen Inn
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- Continental Breakfast
- Free High-Speed Internet
- Free parking
- Pets allowed
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Travel Blogs from Edmonton
Alberta Bound
... and with some excellent displays with recreated shops, dozens of various antique vehicles and farm equipment, and some excellent themed interpretations. We thoroughly enjoyed it.
After lunch we headed downtown, and again found mixed feelings. There is a nice public space and overlook of the Saskatchewan River, with a great family water playground, but there is also major construction of what will be a beautiful new art gallery, but currently is a ...
Back to the future.......
... kinks out I think in the process.
Either way I was up and hosed off and packed and had some time....so went out in search of a cafe. Didn't tale long and found a bakery and was sipping a latte outside. Caught a great sun rise in the process. Kim, the gal at the hotel was awesome an she booked me a ride to the airport on the shuttle....much cheaper option than a taxi...and no where near the pain level of a bus I am sure. So I ended back at the ...
You have a plan so you can change it!!
Well it seems like days since I last posted a blog and a lot has happened here in Canada.
We made it to dog mushing......it was -37c with the chill factor -41!!! My toes were sooooo cold.
Rob and I both sleded around behind the wonderful dogs. I can't post too many pictures as I was too deep in the warmth and the camera and tablet don't like the cold!! Rob had to take the battery out of his camera and ...
In case your wondering yup...I made it home
... and didn't **** off the other folks on the plane in the process. I did play with my seat a lot....it was a bit baffling how it worked...but got comfy for most of me flight and did laps for a while too. Raided the buffet and have a pile of dark chocolate bars in me bag heh heh. The big surprise was upon landing I was pinged that they cancelled me flight home ... ughhhh. Here we go.
Customs was a breeze....and they had already booked me ...
In the Beginning She Asks for Snacks...
Heidi and I had a great time on our last adventure to Seattle in 2012, so we decided we should do it again. After much looking on Google Maps, we decided that we would venture somewhere new, somewhere neither of us has been and somewhere that is driveable, there and back within a week, including sightseeing and shopping; our destination – Boise, Idaho.
"What's in Boise, Idaho," people ...


text:
Xperia Ion
Sony knows electronics, and their engineers are constantly putting new technologies into our everyday devices. The Xperia Ion is no exception, and this incredibly powerful smartphone also gives you full HD quality in your pocket. Here at PureMobile.com you can get accessories that transform your Sony smartphone into a pocket sized computer or the hub of your high definition audio system.
Beats by Dr. Dre 9000001101 Beats Solo HD On-ear Black$197.99
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CyberArk is seeking a full-time in-house legal counsel. This person will act as the primary commercial counsel for our Americas? business and will be instrumental in negotiating commercial transactions with leading global enterprises. This position will report to CyberArk?s8/9/2015 Newton, MA Save This Job
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Location:Cambridge, MA Job Code:1364 # of openings:1 DESCRIPTION Be Part of the Next Generation of Flight Aurora Flight Sciences is a leader in unmanned aerial systems, with a fleet of innovative aircraft designed to empower small tactical units, and safeguard our borders.8/8/2015 Cambridge, MA Save This Job
The Platform Diagnostics and Health Engineer is responsible for enhancing and maintaining the Platform Diagnostics Service, a system which collects, analyzes and reports on diagnostic data from product deployments. Responsibilities i10/10/2015 Waltham, MA Save This Job
HubSpot's Product Marketing team focuses on the positioning, launch strategy, sales and adoption of the full collection of HubSpot products.10/9/2015 Cambridge, MA Save This Job
We're looking for a writer who will focus on growing the audience of the HubSpot Agency blog, targeted at the people working in the agency world. From creative and branding agencies to digital agencies, we're looking for someone who wants10/9/2015 Cambridge, MA Save This Job
A Bit9 + Carbon Black Solutions Architect (SA) delivers effective Advanced Threat Protection and Endpoint Sensor solutions to Bit9 + Carbon Black customers, integrating Bit9 + Carbon Black products with our customers' security programs. The SA inter10/6/2015 Waltham, MA Save This Job
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Are you an "A+" player who likes a challenge Are you passionate about your work Do you like free espresso Will you fight others for the last slice of pizza Do you want to work at a high growth SaaS company in Cambridge, MA, the cradle of high tech Join th9/25/2015 Cambridge, MA Save This Job
Instructional Designer Do you love to enable others to be successful in their role? By joining Bit9 as an Instructional Designer, you will design and develop strategically-aligned learning and development programs for Bit9âs Partner Technical Enablement and Training9/23/2015 Waltham, MA Save This Job
Hubspot is seeking a highly motivated and energetic individual looking to join a growing and fast paced Order Management department. This is a great opportunity for a focused self-starter to join an awesome company and have an impact on the future infrast9/23/2015 Cambridge, MA Save This Job
ResponsibilitiesScheduling maintenance and repair work through to completionEnsuring HubSpot HQ remains a comfortable, safe environment for all staffWeekly facilities inspection and regular follow up with cleaning company to ensure office is maintained to9/18/2015 Cambridge, MA Save This Job
We are looking for someone with measurable experience leading a channel marketing team, an imaginative thinker capable of generating new ideas that will elevate our channel marketing to higher levels.9/9/2015 Waltham, MA Save This Job
HubSpot seeks customer-loving Customer Success Managers to help manage our fast-growing customer base. If being pathologically obsessed with customer experience fits with your professional philosophy we'd love to talk to you9/3/2015 Cambridge, MA Save This Job
At HubSpot we love our customers, period. Our Customer Success Managers are highly praised by our customers for their compulsive level of care and attention and we're just as crazy about them You must be fluent in Spanish to be considered for this ro8/26/2015 Cambridge, MA Save This Job
Bit9 + Carbon Black is currently seeking a dynamic and experienced Technical Product Marketing Manager who will be the primary competitive intelligence expert, technology demonstration architect, and sales tools resource within Marketing at Bit9 + Carbon8/21/2015 Waltham, MA Save This Job
We're seeking a seasoned security professional who has managed software development programs, to coordinate the execution of both internal and external processes to deliver products and services which are resilient against external attack.8/19/2015 Waltham, MA Save This Job
HubSpot is looking for a creative and data-driven marketing manager to support our inbound efforts via our social media and social content efforts.8/18/2015 Cambridge, MA Save This Job
HubSpot is looking for an exceptional front-end web developer to join the marketing team. This is a great opportunity to work on a small team that has a VERY big impact on the entire organization. Our websites are seen by more than 40 million people each8/13/2015 Cambridge, MA Save This Job


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text:
Ratings based on 5 Verified Reviews
They paid and stayed. We double-checked.
4.6 Room cleanliness
5.0 Service & staff
4.6 Room comfort
4.6 Hotel condition
They paid and stayed.
We double-checked.
We double-checked.
for Everyoneby A verified traveler
cheap stayPosted Jul 8, 2015
Pros: upgraded me to an ensuite 3 bedroomed room, clean
Cons: move the whole thing to the Beachfront!?
Location: Blackpool!!
comfortable, friendly staff, clean, secure, parking, facilities
for Everyoneby A verified traveler from CH
Nice hotel close to the beach and main pubsPosted Jul 14, 2014
Host was really helpful and welcoming. Place really nice. And the breakfast was so great and delicious. Great after an amazing night!!
Recommendedby Crazysnakeguy from Cumbria
1 night, good breakfast decent roomPosted Dec 2, 2013 on Hotels
Price & location was right, no problems with stay.
for Couplesby Jenny & Dave from Ascot
glendowie hotelPosted Aug 30, 2013 on Hotels
Very nice hotel, great service. Very friendly and helpful. Room immaculate, you could smell how clean it was. No bad points at all. Breakfast was really good, lots to eat and good size portions. Would definitely stay there again.
for Everyoneby A verified traveler
good hotelPosted Oct 13, 2012
had a very nice stay owners very pleasent in central postion next to bus station


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Publisher:Perseus Books
The author of Windows on the Mind offers a new way of understanding consciousness that takes us out of the old Newtonian world and into the almost mythical realm of contemporary physics--reconciling the cold facts of neuroscience with our most humanistic aspirations.
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text: Slideshows Published On: Jul 06 2012 04:51:34 PM EDT Updated On: Feb 19 2013 05:06:31 PM EST Most 'dangerous' celeb web searches of 2015 10 foods you should never refrigerate Pink fashion: 5K at Marlins Park On this day: October 7 Can you guess the celebrity eyes? On this day: October 6 13 health rules you can break 10 practical back-to-school gadgets 10 stylish backpacks for teenage girls 25 best places to retire Pets available for adoption On this day: October 5 World Beard and Moustache Championships Where are 'Cosby Show' stars today? 10 cool T-shirts for older teenage boys 10 trendy sneakers for teenage girls Historic flooding swamps South Carolina Orphaned baby possum snuggles with toy kangaroo PHOTOS: White hot Heat fans Timeline: El Faro sinking Celebrity breakups of 2015 PHOTOS: Fans celebrate Miami Heat championship 14 surprising foods you should refrigerate 20 most boring cities in America By the numbers: Americans who work from home 10 biggest strikes in U.S. history My Favorite Things: Janine Stanwood PHOTOS: Miami Broward 2012 Carnival Victor Oquendo Through The Years Laurie Jennings Through The Years View More


text:
The comedian is cast as the clown prince of crime.
The Pixar favorite finally comes to fruition through LEGO® Ideas.
The iconic sneaker gets recreated in plastic brick form.
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This is the most detailed LEGO® build of recent memory.
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This year's Hopkinton Polyarts festival will take place on the Town Common Sept. 15, 10 a.m. to 4 p.m.
This is a daylong, free admission showcase of local artists. The event features a large craft fair, where vendors will sell a variety of items, including jewelry, paintings, caricatures, handmade clothing, purses, and accessories, glass and metal art and dishware, as well as paper products and natural soaps.
There will be food vendors and homemade desserts, children's activities, as well as music and other entertainment.
Proceeds from the event fund $1,000 scholarships for two Hopkinton residents pursuing a college degree in the arts.
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text:
More Labrador Retriever Puppies and Dogs for sale.
INT FTCH APACHE JOE X TYRRELLISON Z... Tyronisson Labrador Retrievers in Kiln, Mississippi
Started Labrador Retrievers for sal... Sporting`Life British Kennels in Oxford, Mississippi
White English Pups...great hunting ... Beloved Labs in Roosevelt, Minnesota
AKC Chocolate lab puppies, champion line... Snake River Retrievers in Saint Anthony, Idaho
STUNNING BLACK FEMALE - PHENOMENAL ... Brown's Spring River Retrievers in Monett, Missouri
Fox red female, 7 months Rainmaker Labs in Stone Lake, Wisconsin
Chocolate male, 7 months Rainmaker Labs in Stone Lake, Wisconsin
HRCH TJ BOOKER MH X MORGAN ALL CHO... Hill Top Acres in Lancing, Tennessee
AKC Black/Chocolate Lab pups due Oc... Harvest Moon Farm and Kennels in Boyceville, Wisconsin
Yellow Pointing Labradors Duckflat Retrievers in Albany, Oregon
HANDSOME BLACK MALE - "PHENOMENAL"... Brown's Spring River Retrievers in Monett, Missouri
Black/Chocolate Lab Pups - Kansas Circle Seven Gun Dogs in Elk City, Kansas
4XGMPR Sired Pointing Lab Pups Spring Creek Kennels in Newtown, Missouri
Chocolate pups ready to go now! Furzland's Field Labradors in Princeton, Minnesota
LABRADOR PUPPIES Bird Crazy Gun Dogs in Portage, Wisconsin
AKC Labrador Puppies Eagle Creek Labs in Eden Valley, Minnesota
Very Sweet CKC Registered Black Fem... Johnson's Labrador Retrievers in Murfreesboro, Tennessee
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AKC Lab Puppies, Awesome Hunting An... Johnson's Labrador Retrievers in Murfreesboro, Tennessee
Started Labrador Retrievers Sporting`Life British Kennels in Oxford, Mississippi
Started British Lab Champion Bloodlines Cochran British Labradors in Willow Springs, Missouri
See Our Current Ad Brown's Spring River Retrievers in Monett, Missouri
Labrador clearance sale yellows and... Walnut Ridge Quail Farm and Kennel in Pulaski, Tennessee
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Chocolate Lab Puppies ready 10/31 Hillcountry Hunting Labs in Wimberely, Texas
AKC Light Cream Lab Puppies Tovah Labradors in PALMER LAKE, Colorado
AKC Master Hunter And Junior Hunter Lab Puppies, Upcoming Litter!!! - Labrador Retrievers in Murfreesboro, Tennessee - Johnson's Labrador Retrievers
AKC Master hunter and Junior hunter pure breed Labrador Retriever Puppies, upcoming litter. The puppies
are due to be born September 27th, 2014. We are expecting all colors chocolate, yellow and black. We could
also have fox red puppies. The puppies will be ready to go to their new homes November 8th, 2014. The
puppies will come with their 1st set of shots. They will have had their dew claws removed and will be
dewormed at 2, 4 and 6 weeks of age. The puppies will come with FULL AKC Registration papers. The
prices are $800 for blacks, $800 for yellows and $1,000 for chocolates. The puppies will come with a 24
month written health guarantee. The puppies will be around kids and other pets. We are accepting $200
non-refundable deposits now! The deposit comes off the total price of the puppy. The mom is chocolate
in color, she is AKC Registered. She has (MH) Which means (Master Hunter) and (JH) which means
(Junior Hunter) in her bloodlines. Kylie is 60 pounds. She is very smart and loves the water. The Dad is
AKC and CKC Registered and has (JH) which means (Junior Hunter) and (CD) which means
(Companion Dog) in his bloodlines. Dakota is 85 pounds and is fox red in color and loves the water.
He has a block head and a really nice body build. Call or text me (Kevin) at (256) 417-9638, If you
would like to put a deposit down on one of the puppies. We now take any major credit card or debit
card as a payment option. It is very safe and secure. We don't get your info, it goes right to Pay-Pal and
you don't have to have a Pay-Pal account to make the payment. You can also come out in person
and put your deposit down in cash, it's up to you. The puppies, mom and dad are in Mt. Juliet,
Tennessee and that's where you would come and get the puppy. If you are to far away to come and
get the puppy I can deliver the puppy to you personally myself for a reasonable fee. I have been
breeding dogs for many years and I love it very much. I have a business license to raise and sell
puppies in the state of Tennessee called Johnson's Labrador Retrievers. Check out my website at
http://www.johnsonslabradorretrievers.com Thank you and God Bless!
Ad location: Murfreesboro, Tennessee
Ad created: Aug 06, 2014
Contact Information
Contact Name: Kevin
256-417-9638Contact breeder through email Click here to visit website
Johnson's Labrador Retrievers Breeder Information
My Classified Pictures


text:
Hotel President, Giovinazzo
- Free WiFi and free parking
Main amenities
- 68 guestrooms
- Restaurant and bar/lounge
- Outdoor pool
- Rooftop terrace
- Business center
- Airport shuttle
- Childcare
- 24-hour front desk
- Coffee/tea in the lobby
- Air conditioning
- Garden
- Library
What's around
- On the waterfront
- Planetarium of Bari (6.5 mi / 10.4 km)
- Cathedral of St. Conrad (6.6 mi / 10.7 km)
- Angevin Tower (7.9 mi / 12.7 km)
- Bitonto Cathedreal (8 mi / 12.9 km)
- Lido San Francesco (8.4 mi / 13.5 km)
Pool is open during day and good condition. The staff were very polite and helpful. All…
Lovely large family room with balcony and sea view. Very helpful reception staff.
Enter dates to check prices
Hotel Info: 020 3027 9803from £38
- Double Room Single Use, Sea View
- Double Room
- Single Room
- Double Room, Sea View
- Quadruple Room
- Standard Double Room Single Use
- Triple Room
Also popular with travellers from the UK
At a glance
Key facts
Hotel size
- This hotel has 68 rooms
- This hotel is arranged over 4 floors
- Check-in time noon-5:30 AM
- Check-out time is 11 AM
- Express check-in
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Babysitting/childcare *
- Pets allowed
- Restrictions apply *
- 1 per room
Extra people
- Rollaway beds available, charges may apply*
- Free WiFi in public areas
- Free WiFi in rooms
- Airport shuttle (available on request) *
- Free self parking
- Accessible parking
Nearby Four Star Giovinazzo hotels
In the hotel
- Restaurant
- Bar/lounge
- Room service (during limited hours)
- Free reception
- Coffee/tea in the lobby
- Outdoor pool
- Pool sun loungers
- Bicycle rentals nearby
- Pool umbrellas
- Business center
- Number of meeting rooms - 3
- Conference space
- 24-hour front desk
- Concierge services
- Tours/ticket assistance
- Dry cleaning/laundry service
- Laundry facilities
- Free newspapers in lobby
- Luggage storage
- Number of buildings/towers - 1
- Year Built 2004
- Elevator/lift
- Rooftop terrace
- Garden
- Library
- Disabled parking
- Accessible path of travel
- Braille or raised signage
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- Climate control
- Air conditioning
- Minibar
- Soundproofed rooms
- Private bathroom
- Bathtub or shower
- Free toiletries
- Hair dryer
- Pay movies
- Satellite TV channels
- Desk
- Free WiFi
- Refrigerator
- In-room safe
- Connecting/adjoining rooms available
Top Giovinazzo hotels
Recently reviewed hotels in Giovinazzo
- Oriente HotelFrom
"Really enjoyed my stay. Lovely room, easy check in and I was early and still given my room. Great varied breakfast, friendly staff. Didn't have my toileteries replaced on the…"Sep 3, 2015
- Barion HotelFrom
"Great hotel. Awesome buffet breakfast on the rooftop terrace. The beds are extremely uncomfortable as are the pillows. We were so tired one of the nights that we didn't really…"Aug 12, 2015
- FabrisPalace ResidenceFrom
"Hidden in old quarter of Trani,we had a serious car problem invovling the italian version of the bomb squad. We had police in protective gear all over the car.The receptionist and…"May 11, 2015
- Hotel MajestyFrom
"Nice hotel, but car needed as no nearby cafes or restaurants. 20 mins Drive to Bari Centre. Reception staff very helpful, but breakfast staff not and eager to clear well before…"May 6, 2015
Nearby hotels
Special features
DiningRistorante Medi - Overlooking the garden, this restaurant specializes in Italian cuisine and serves breakfast, lunch, dinner, and light fare. Guests can order drinks at the bar and enjoy alfresco dining (weather permitting). A children's menu is available. Open select days.
Hotel President, Giovinazzo's small print
Cash transactions at this property cannot exceed EUR 999.99, due to national regulations. For further details, please contact the property using information in the booking confirmation.
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
Pet-friendly rooms can be requested by contacting the property at the number on the booking confirmation.
Optional extras
Rollaway beds are available for EUR 40 per night
Babysitting/childcare is available for an extra charge
Airport shuttle service is offered for an extra charge
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


text:
Mercedes-Benz Repair in Lititz
Our master mechanics identify the best Mercedes-Benz repair shops in Lititz. Edit search×
There are 21 Mercedes-Benz service shops and mechanics in Lititz. Edit search
RepairPal has not certified the following shops.
Find RepairPal Certified Mercedes-Benz repair shops in Marietta, Reading and Coatesville.
The shops listed below are not committed to the fair price guarantee and minimum warranty of RepairPal Certified shops. When taking your car to a shop that has not passed RepairPal's inspection, we recommend that you:
- Look for a shop with ASE-certified technicians.
- Request written estimates from multiple shops and check them with the RepairPal estimator
- Verify that the shop you select is willing and able to clearly explain the work being performed.
Mercedes-Benz repair shops and dealerships near Lititz.
1038 Newport Rd, Manheim
Muffler & Exhaust, Oil Change & Lube, Roadside Assistance, Service & Repair, Tire Sales/Repair, Transmission Repair, Windshield/Glass
"Cool Guys"
23a Owl Hill Rd, Lititz
"This shop is what you always hope to find but hardly ever do. They are scrupulously honest, do good work, try to keep..."
1603 Rothsville Rd, Lititz
Oil Change & Lube, Service & Repair
"Fred High and his team has been our family mechanic for over 25 years. Honesty is the most important quality I look f..."
126 East 28th Divided Highway, Lititz
Muffler & Exhaust, Service & Repair
5988 Main St., E Petersburg
Muffler & Exhaust, Service & Repair
1229 Brunnerville Rd, Lititz
Service & Repair, Tire Sales/Repair
306 W. Newport Rd, Lititz
734 Conestoga Ave, Manheim
Service & Repair, Tire Sales/Repair
253 Redwood Land, Manheim
3240 Oregon Pike, Leola
467 E Main St, Lititz
2671 Lititz Pike, Lancaster
140 Front St, Lititz
124 W End Dr, Manheim
235 S. Charlotte St, Monheim
746 S. Broad Street, Lititz
Oil Change & Lube, Service & Repair
737 S Broad St, Lititz
"I had a 1991 toyota carmy and I had no
brake lights and owner tested everything
possible and could Not Fix the promle..."
2665 Lititz Pike, Lancaster
533 E Newport Rd, Lititz
33 Industrial Rd, Ephrata
AC/Heating, Alignment, Check Engine Light, Collision Repair & Paint, Diagnosis, Hybrid/Electric, Muffler & Exhaust, Service & Repair, Windshield/Glass
Test Shop, Doesn't Really Exist
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text: Dear WikiMail, The PCGen Wiki page Main Page has been changed on 22 June 2012 by Papa.DRB, see http://wiki.pcgen.org/Main_Page for the current revision. See http://wiki.pcgen.org/index.php?title=Main_Page&diff=0&oldid=3099 for all changes since your last visit. Editor's summary: /* Latest News! */ Contact the editor: mail: http://wiki.pcgen.org/Special:EmailUser/Papa.DRB wiki: http://wiki.pcgen.org/User:Papa.DRB There will be no other notifications in case of further changes unless you visit this page. You could also reset the notification flags for all your watched pages on your watchlist. Your friendly PCGen Wiki notification system -- To change your watchlist settings, visit http://wiki.pcgen.org/Special:Watchlist/edit To delete the page from your watchlist, visit http://wiki.pcgen.org/index.php?title=Main_Page&action=unwatch Feedback and further assistance: http://wiki.pcgen.org/Help:Contents


text: Reviews Toys and gifts For children with special needs No Yes For children with special needs Search by keyword Add a review Choose By Brand Fatframe(1) Ravensburger(1) Something Special(1) The Boingy(1) The Sensory Spot(1) Choose By Multisensory No(4) Yes(1) Choose By Visual No(1) Yes(4) Choose By Auditory No(4) Yes(1) Choose By Tactile No(1) Yes(4) Choose By Vestibular No(5) Yes(0) Choose By Proprioception No No(5) Yes(0) Choose By Fine motor No(2) Yes(3) Choose By Gross motor No(4) Yes(1) Choose By Cognition No(2) Yes(3) Choose By Communication No(3) Yes(2) Choose By Socialisation Yes No(2) Yes(3) Choose By Independence No(3) Yes(2) Choose By Type For children with special needs Toys for babies Toys and games for toddlers and preschoolers Toys and games for 5-7 year-olds Toys and games for 8-11 year-olds Family board games Gifts for newborn babies For children with special needs Outdoor toys and equipment Pregnancy gifts Gifts for new parents Naming ceremony and Christening gifts Gifts for mums Gifts for dads Be a Mumsnet reviewer Interested in joining our review panel? Businesses How to get your product reviewed on Mumsnet. Star rating Number of reviews Mumsnet for children with special needs reviews No Yes Fatframe iPad case 4 reviews Ravensburger Snails Pace Race 1 review The Boingy Boingy Cushions 1 review


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text:
FPS Freek CQC Signature
Make 'em GREEN with ENVY
Customer Reviews Write a Review
Simply Awesome
By SiTh LoRdThese things are great and a must have, after 3 games I was ready and it Def helped my kd..the control and accuracy are top notch, I'll never use a controller without them again.
By fdfdfI find this perfect for my left stick, would recommend pairing this with the vortex high stick
Amazing !
By Dr ExilesThese are the perfect height for guys with smaller hands, perfect for sniping and reg gunning. I was sceptical at first but the hype is real, these things are amazing. Peace
» See all 250 customer reviews
Questions and Answers
NOTE: This Q&A is for FPS Freek CQC Signature questions only.
For other questions, follow these links:
Questions About Your Order
Contact Us
Frequently Asked Questions (FAQS)
Q: How do you use discount codes?
Posted On: 5/9/15 By: jordan
A: At checkout, there will be a box on the right hand side.
Q: Someone already asked about the height difference between the CQC Signatures and the default CQCs, but is there a height difference between the CQC Signatures and the CQC Xs? It looks like it from the pictures, but I can't really tell.
Posted On: 3/23/15 By: Nile
A: Yes, there is, but just slightly. The CQC and CQC Signatures are the same height (0.30), while the CQCX are taller (0.33).
Q: is there a height difference between these are the regular CQCs?
Posted On: 9/12/14 By: Eric
A: Overall, no. However CQC Sig is concave/convex while CQC is just concave, so the convex portion of CQC Sig will have a little added height.
Q: Does this work on xbox 360? Or just Ps3
Posted On: 9/8/14 By: brayden castillo
A: The XBOX360/PS3 size works on both systems.
Q: I have small hands which one should i buy
Posted On: 8/1/14 By:
A: CQC or CQC Sig!
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Don't forget to check your spam folder if you cannot find the email. For further assistance please contact us at email@example.com .Okay
Antique & Art Exchange
Antique; 19th century
Very Good; very minor wear and rubbing to spine, especially for age.
7" L x 5" W
San Francisco's Antique & Art Exchange was founded in 1995 by Keith Dieterich and Howard Settle. The shop was an immediate success, promptly credited by design legend Tony Hail in a Town & Country article as having "practically nothing ugly." Today its clientele boasts heads of state, politicians, billionaires, writers, artists, celebs, dealers, and (naturally) designers, all of whom flock to the Design District showroom to shop for antiquities, modern pieces, and everything in between.


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text:
Type: Radio
Issues: Civil Liberties/ Human Rights, Free Market Economics
Randy Simmons | The True Costs of Green Energy
Recorded: Monday, April 27, 2015
Senior Fellow Randy Simmons joins WSVA radio's Mike Schikman to talk about the true costs of green energy including government funding and incentives for renewable energy.
Experts: Randy T Simmons
Type: Radio
Issues: Bureaucracy and Government, Energy, Environment, Free Market Economics, Property Rights, Public Choice Theory, Regulation and Deregulation
Ivan Eland on the Iranian Nuclear Agreement
Recorded: Monday, April 6, 2015
Sr. Fellow Ivan Eland appears on the America Tonight radio show with Kate Delaney. Eland discusses the implications of the framework of a deal with Iran to halt their nuclear weapon development.
Experts: Ivan Eland
Type: Radio
Issues: Defense and Foreign Policy, Diplomacy and Foreign Aid
Partitioning Iraq for Peace
Recorded: Tuesday, March 31, 2015
Senior Fellow Ivan Eland, author of Partitioning for Peace, appeared on "Doug Stephan's Good Day" radio program. Eland talks about the state of terrorism in the Iraq and whether the US creates more of a problem with its intervention. He also discusses the question of how ISIS is different than other terror groups.
Experts: Ivan Eland
Type: Radio
Issues: Civil Liberties/ Human Rights, Culture/ Society, Defense and Foreign Policy, Diplomacy and Foreign Aid, Freedom, History (International), Nationalism, Politics, Race Issues, Religion, Terrorism and National Crises
Why Should We Care If Iran has a Nuclear Bomb?
Recorded: Monday, March 30, 2015
Sr. Fellow Ivan Eland appeared on the Larry Conners USA Radio program to talk about the negotiations with Iran over their nuclear capabilities. Eland believes that the U.S. should give negotiations a chance to work and that any military action the U.S. or Israel might take would require an invasion of Iran. Airstrikes alone would not stop Iran from developing a nuclear weapon.
Experts: Ivan Eland
Type: Radio
Issues: Defense and Foreign Policy, Diplomacy and Foreign Aid, Terrorism and National Crises
|1 | 2 | 3 | 4 | 5 | 6 | 7
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text:
Additional file 4.
Table of Singleplex PCR primers used in this study. The first and fourth columns give the 5' ends of the forward and reverse primers, respectively.
Format: PDF Size: 676KB Download file
This file can be viewed with: Adobe Acrobat Reader
Endicott et al. BMC Genetics 2009 10:29 doi:10.1186/1471-2156-10-29


text:
The AC-10000H (http://www.air-
According to Jocelyn Anne, marketing associate for Air & Water, exclusive distributor of all New Air products, the product was officially released in May and has been exceeding their expectations as well as their customers ever since. "We'
It's not only compact, it's one of the most lightweight products available, making it perfect for moving between bedrooms, small house spaces, even office cubicles. Rolling casters make sliding it a breeze ensuring it can be used whenever and wherever needed. Plus, the built in 12 hour programmable timer helps to conserve energy even further, ensuring it only runs as needed. The AC 10000H also boasts the eco-friendly R-410A refrigerant that is safe for the ozone.
As stated, NewAir products are currently only available through the online retailer, Air & Water, one of the country's largest independent retailers of residential and commercial heating, cooling and purification products. You can view their entire line of New Air products on their website http://www.air-
# # #
Air & Water, Inc. is located in Southern California and was established in 2002. As one of the Internet's largest independent appliance retailers, the company has helped thousands of customers across the country find home environment solutions and appliances. When it comes to residential and commercial heating and cooling products, Air & Water's goal has always been to make your experience the way online shopping should be.