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|By Marketwired .
Article Rating:
|July 30, 2014 05:30 PM EDT
TORONTO, ONTARIO -- (Marketwired) -- 07/30/14 -- Lundin Mining Corporation (TSX:LUN)(OMX:LUMI) ("Lundin Mining" or the "Company") today reported net earnings of $39.7 million ($0.07 per share) for the quarter ended June 30, 2014. Cash flows of $33.7 million were generated from operations in the quarter, not including the Company's attributable cash flows from Tenke Fungurume.
Paul Conibear, President and CEO commented, "We are pleased with our improvements in operating performance during this last quarter and we remain very well positioned to deliver another year of strong operating results from all our mines, with production and costs as expected or in some cases better than original guidance.
"As announced on July 16th, we are very excited with the construction progress at the Eagle Mine, with the delivery of first ore to the mill achieved earlier in the month. The Eagle Mine is expected to be in production in early Q4, and that combined with excellent nickel, copper and zinc price environments is expected to generate strong cash flows from all of our mines as the year advances."
Summary financial results for the quarter and year-to-date:
---------------------------------------------------------------------------- Three months ended Six months ended June 30, June 30, ------------------- ------------------- US$ Millions (except per share amounts) 2014 2013 2014 2013 ---------------------------------------------------------------------------- Sales 191.8 176.3 341.7 364.4 Operating earnings(1) 74.2 49.2 117.3 117.2 Net earnings 39.7 16.6 53.1 66.6 Basic earnings per share 0.07 0.03 0.09 0.11 Cash flow from operations 33.7 26.6 61.2 72.4 Ending net (debt) / cash position(2) (174.4) 221.1 (174.4) 221.1 ---------------------------------------------------------------------------- (1) Operating earnings is a non-GAAP measure defined as sales, less operating costs (excluding depreciation) and general and administrative costs. (2) Net cash/debt is a non-GAAP measure defined as cash, less long-term debt and finance leases, before deferred financing fees.
Operational Performance
Wholly-owned operations: Nickel and lead production exceeded expectations, while copper and zinc production were in-line with targeted production for the quarter. Higher throughput at Aguablanca resulted in better than expected nickel and copper production, while higher lead grades and throughput at Zinkgruvan resulted in higher lead production. Copper, nickel and lead production guidance has been increased reflecting excellent mine production at Aguablanca, higher throughput at Zinkgruvan and better than expected lead grades from the Lombador ore body at Neves-Corvo.
-- Neves-Corvo produced 13,480 tonnes of copper and 17,909 tonnes of zinc in the second quarter of 2014. Production from the Lombador ore body resulted in a 28% increase in zinc production over the prior year comparable period. Higher copper head grades were more than offset by lower metallurgical recoveries and ore throughput, resulting in lower copper production compared with the second quarter of 2013, but in-line with our mine plan for 2014. Copper cash costs(1) of $1.62/lb for the quarter were lower than original guidance ($1.90/lb) due primarily to higher than expected zinc and lead by-product credits, net of treatment charges. -- Zinc production of 19,293 tonnes at Zinkgruvan met expectations and was slightly higher than the comparable period in 2013 due to record mine production and mill throughput of zinc ore. Lead production of 9,196 tonnes exceeded expectations but was below the comparable period in 2013 primarily due to lower head grades. Cash costs for zinc of $0.17/lb were lower than guidance ($0.35/lb) in part due to higher lead by-product credits. -- Aguablanca had another strong quarter of operational performance, with current quarter production of 2,212 tonnes of nickel and 1,799 tonnes of copper. This exceeded both expectations for the second quarter of 2014 and production levels of the prior year comparable period. Cash costs of $5.05/lb of nickel for the quarter, though higher than original guidance, remain in-line with our mine plan.
Tenke: Tenke operations continue to perform well.
-- Lundin's attributable share of second quarter production included 12,449 tonnes of copper cathode and 820 tonnes of cobalt in hydroxide. The Company's attributable share of Tenke's sales included 12,810 tonnes of copper at an average realized price of $3.08/lb and 734 tonnes of cobalt at an average realized price of $9.58/lb. -- Attributable operating cash flow from Tenke for the second quarter of 2014 was $37.8 million ($65.5 million year-to-date). Cash distributions received by Lundin Mining in the quarter were $22.6 million ($39.3 million year-to-date), lower than expected due to timing of shipments and lower copper price. -- Operating cash costs for the second quarter of 2014 were $1.18/lb of copper sold, better than the original full year guidance of $1.22/lb. -- The Company is also pleased to announce that it has filed an independent technical report for its Tenke Fungurume Mine entitled "Technical Report - Resource and Reserve Update for the Tenke Fungurume Mine, Katanga Province, Democratic Republic of Congo" authored by John Nilsson, P.Eng. and Ron Simpson, P.Geo. and dated effective as of July 21, 2014.
(1) Cash cost/lb of copper, zinc and nickel are non-GAAP measures defined as all cash costs directly attributable to mining operating, less royalties and by-product credits.
Eagle Nickel/Copper Project: advancing on time and on budget.
-- Mine surface construction is complete, and there are approximately 500 people currently working at the mill, including contractors. All of the major equipment has been installed. As of June 30, 2014, mill construction was progressing as planned at 90% completion. -- Capital costs are on budget, expecting to come in at the original forecast of $400 million from the date of acquisition. $225 million has been spent since that time, of which $65 million was spent in the second quarter of 2014. -- County road upgrade work re-started in May and is on track to be completed as planned. -- Operations and maintenance hiring is complete and training is underway. -- Mine development in ore has begun and mill commissioning is expected to start in the third quarter of 2014. Eagle is on track to ship first saleable copper and nickel concentrates in the fourth quarter of 2014. Ore processing and concentrate production are expected to reach full design rates in the second quarter of 2015.
Total production from the Company's assets including attributable share of Tenke:
---------------------------------------------------------------------------- 2014 2013 (tonnes) YTD Q2 Q1 Total Q4 Q3 Q2 Q1 ---------------------------------------------------------------------------- Copper 31,582 16,182 15,400 66,246 18,078 15,087 16,065 17,016 Zinc 70,669 37,202 33,467 124,748 32,796 33,466 32,539 25,947 Lead 20,188 10,250 9,938 34,370 7,968 9,119 10,692 6,591 Nickel 4,192 2,212 1,980 7,574 2,113 1,788 1,876 1,797 ---------------------------------------------------------------------------- Tenke attributable Copper 24,320 12,449 11,871 50,346 12,155 11,890 13,230 13,071 ----------------------------------------------------------------------------
Financial Performance
-- Operating earnings for the second quarter of 2014 were $74.2 million, an increase of $25.0 million from the $49.2 million reported in the comparable quarter of 2013. The increase was largely attributable to higher net realized metal prices and prior period price adjustments ($33.4 million), partially offset by lower sales volume ($10.8 million). On a year-to-date basis, operating earnings of $117.3 million were consistent with the $117.2 million reported for the first six months of 2013. Lower production costs ($22.2 million) were offset by lower sales volumes ($24.4 million). -- For the quarter ended June 30, 2014, sales of $191.8 million increased $15.5 million over the prior year quarter ($176.3 million) as a result of higher net realized metal prices and prior period price adjustments ($33.4 million), partially offset by lower net sales volumes ($13.4 million), primarily at Neves-Corvo. Sales of $341.7 million for the six months ended June 30, 2014 were $22.7 million lower than the comparable period in 2013 ($364.4 million) due to lower net sales volumes ($33.5 million), primarily at Neves- Corvo, partially offset by higher net realized metal prices and prior period price adjustments ($17.5 million). -- Average metal prices for zinc, lead and nickel for the three months ended June 30, 2014 were higher (2% - 23%) than the same period in the prior year, while copper prices declined slightly from the prior year comparable period (5%). Average metal prices for zinc and nickel for the six months ended June 30, 2014 were higher (2% - 6%) than the same period in the prior year, while copper and lead prices declined from the prior year comparable period (4% - 8%). -- Operating costs (excluding depreciation) of $111.0 million in the current quarter were $11.6 million lower than the prior year comparative quarter of $122.6 million due to the closure of our Galmoy operation and lower sales volumes at Neves-Corvo. Operating costs (excluding depreciation) of $211.2 million year-to-date were $24.9 million lower than the prior year of $236.1 million due to the closure of our Galmoy operation and lower sales volumes at Neves- Corvo and Aguablanca, partially offset by higher sales volumes at Zinkgruvan. -- Net earnings of $39.7 million ($0.07 per share) for the three months ended June 30, 2014 were $23.1 million higher than the $16.6 million ($0.03 per share) reported in the comparable quarter in 2013. Net earnings were positively impacted by higher operating earnings ($25.0 million) largely due to higher net realized metal prices and prior period price adjustments. Net earnings of $53.1 million ($0.09 per share) year-to-date were $13.5 million lower than the $66.6 million ($0.11 per share) reported in 2013. In 2013, earnings benefited from $15.1 million in insurance proceeds for business interruption at the Aguablanca mine and higher equity income from our investment in Tenke Fungurume ($7.3 million) 2014, partially offset by higher foreign exchange losses ($6.7 million). -- Cash flow from operations for the current quarter was $33.7 million compared to $26.6 million for the same period in 2013. The increase in the cash flow of $7.1 million is mostly attributable to higher operating earnings, partially offset by changes in non-cash working capital. For the six months ended June 30, 2014, cash flow from operations was $61.2 million compared to $72.4 million for the same period in 2013. Change in non-cash working capital was the primary contributor to the decrease.
Corporate Highlights
-- On July 16, 2014, the Company provided an update on the final stages of construction and commencement of mine operations at the high grade Eagle nickel-copper project, as well as an exploration update on the adjacent Eagle East nickel-copper deposit, located in the Upper Peninsula of Michigan, USA. The project reached a key milestone, delivering its first ore from mine to mill for plant commissioning. Since then, testing of crushing, conveying and grinding circuits has started under no-load conditions in preparation for introduction of feed for full system commissioning in the month ahead. With these achievements the project remains well on schedule for first concentrate production early in the fourth quarter of 2014. See press release entitled "Lundin Mining Announces First Ore Shipment at Eagle Mine", dated July 16, 2014.
Financial Position and Financing
-- Net debt position at June 30, 2014 was $174.4 million compared to $119.3 million at December 31, 2013 and $155.0 million at March 31, 2014. -- The $19.4 million increase in net debt during the quarter was attributable to investments in mineral properties, plant and equipment of $99.3 million, primarily the development of the Eagle project, partially offset by operating cash flows of $33.7 million, distributions from Tenke and Freeport Cobalt of $22.6 million and $7.2 million, respectively, and an $11.7 million withdrawal from restricted funds. For the six months ended June 30, 2014, net debt increased $55.1 million due to investments in mineral properties, plant and equipment of $191.7 million, primarily the development of the Eagle project, partially offset by operating cash flows of $61.2 million, distributions from Tenke and Freeport Cobalt of $39.3 million and $7.2 million, respectively, and a $22.5 million withdrawal from restricted funds. -- The Company has corporate term and revolving debt facilities available for borrowing up to $600 million. At June 30, 2014 the Company had $321.9 million committed against these facilities, leaving debt capacity of $278.1 million available for future drawdowns.
2014 Production and Cost Guidance
-- Production and cash costs guidance for 2014 for the Company's wholly- owned operations have been adjusted to reflect excellent production performance at Aguablanca and Zinkgruvan, and higher lead grades from our Lombador ore body at the Neves-Corvo mine. -- Guidance on Tenke's cash costs have been updated to reflect the most recent guidance provided by Freeport-McMoRan Inc. ("Freeport"). Tenke production guidance remains unchanged. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- 2014 Guidance Prior Guidance Revised Guidance C1 (contained tonnes) Tonnes C1 Cost Tonnes Cost(a) ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- Copper Neves-Corvo 50,000 - 55,000 $1.90/lb 50,000 - 55,000 $1.85/lb Zinkgruvan 3,000 - 4,000 3,000 - 4,000 Aguablanca 5,000 - 6,000 6,000 - 7,000 Eagle 2,000 - 3,000 2,000 - 3,000 --------------------------------------------------------------------- Wholly-owned 60,000 - 68,000 61,000 - 69,000 Tenke (@24%)(b) 47,900 $1.22/lb 47,900 $1.21/lb --------------------------------------------------------------------- Total attributable 107,900 - 115,900 108,900 - 116,900 Zinc Neves-Corvo 60,000 - 65,000 60,000 - 65,000 Zinkgruvan 75,000 - 80,000 $0.35/lb 75,000 - 80,000 $0.35/lb --------------------------------------------------------------------- Total 135,000 - 145,000 135,000 - 145,000 Lead Neves-Corvo 2,000 - 2,500 3,500 - 4,500 Zinkgruvan 27,000 - 30,000 29,000 - 32,000 --------------------------------------------------------------------- Total 29,000 - 32,500 32,500 - 36,500 Nickel Aguablanca 6,000 - 7,000 $4.50/lb 7,500 - 8,500 $4.25/lb Eagle 2,000 - 3,000 2,000 - 3,000 --------------------------------------------------------------------- Total 8,000 - 10,000 9,500 - 11,500 ---------------------------------------------------------------------------- a. Cash costs remain dependent upon exchange rates (forecast at EUR/USD:1.35, USD/SEK:6.50) and metal prices (forecast at Cu: $3.15/lb, Zn: $0.90/lb, Pb: $0.95/lb, Ni: $7.50/lb, Co: $13.00/lb). Prior guidance forecast Ni at $6.50/lb and Co at $12.00/lb. b. Freeport has provided 2014 sales and cash costs guidance. Tenke's 2014 production is assumed to approximate sales guidance.
2014 Capital Expenditure Guidance
Capital expenditures for 2014 are expected to be $440 million (including Eagle, but excluding Tenke), unchanged from previous guidance. Major capital investments for 2014 are as follows:
-- Sustaining capital in European operations - $100 million, consisting of approximately $60 million for Neves-Corvo, $35 million for Zinkgruvan and $5 million across other sites. -- Expansionary capital in European operations - $40 million, consisting of: -- Lombador - $25 million: For underground vertical and horizontal development and associated mine infrastructure related to the development of the upper Lombador ore bodies for future high grade zinc and copper production. Redesign and optimization of development has allowed for a combination of cost savings and the deferral of certain expenditures into 2015. -- Neves-Corvo zinc plant debottlenecking and zinc expansion studies - $5 million: For the installation of a zinc tailings recovery circuit, zinc expansion feasibility studies and Santa Barbara hoisting shaft capacity increase design work. -- Aguablanca underground mining project - $10 million: For ramp and initial ore body development and the installation of associated mine infrastructure. -- New investment in Eagle project - $300 million, to complete construction of the Humboldt mill and Eagle mine. -- New investment in Tenke - $50 million, estimated by the Company as its share of expansion related initiatives and sustaining capital funding for 2014. All of the capital expenditures are expected to be self-funded by cash flow from Tenke operations. The Company believes it is reasonable to expect Lundin's attributable cash distributions from Tenke to be in the range of $80 to $100 million in 2014, potentially lower than previous guidance due to copper price uncertainty and higher working capital.
2014 Exploration Guidance
-- Total exploration expenses for 2014 (excluding Tenke) are estimated to be $35 million, consistent with prior guidance. These expenditures will be principally directed towards underground and surface mine exploration at Neves-Corvo, Zinkgruvan and Eagle, select greenfield exploration programs and new business development activities in South America and Eastern Europe.
About Lundin Mining
Lundin Mining Corporation is a diversified Canadian base metals mining company with operations and development projects in Portugal, Sweden and Spain and the USA, producing copper, zinc, lead and nickel. In addition, Lundin Mining holds a 24% equity stake in the world-class Tenke Fungurume copper/cobalt mine in the Democratic Republic of Congo and in the Freeport Cobalt Oy business, which includes a cobalt refinery located in Kokkola, Finland.
On Behalf of the Board,
Paul Conibear, President and CEO
Forward-Looking Statements
Certain of the statements made and information contained herein is "forward-looking information" within the meaning of the Ontario Securities Act. This report includes, but is not limited to, forward looking statements with respect to the Company's estimated full year metal production, cash costs, exploration expenditures, and capital expenditures, as noted in the Outlook section and elsewhere in this document. These estimates and other forward-looking statements are based on a number of assumptions and are subject to a variety of risks and uncertainties which could cause actual events or results to differ from those reflected in the forward-looking statements, including, without limitation, risks and uncertainties relating to the estimated cash costs, timing and amount of production from the Eagle project, cost estimates for the Eagle project, foreign currency fluctuations; risks inherent in mining including environmental hazards, industrial accidents, unusual or unexpected geological formations, ground control problems and flooding; risks associated with the estimation of mineral resources and reserves and the geology, grade and continuity of mineral deposits; the possibility that future exploration, development or mining results will not be consistent with the Company's expectations; the potential for and effects of labour disputes or other unanticipated difficulties with or shortages of labour or interruptions in production; actual ore mined varying from estimates of grade, tonnage, dilution and metallurgical and other characteristics; the inherent uncertainty of production and cost estimates and the potential for unexpected costs and expenses, commodity price fluctuations; uncertain political and economic environments; changes in laws or policies, foreign taxation, delays or the inability to obtain necessary governmental permits; litigation risks; and other risks and uncertainties, including those described in the Risk and Uncertainties section of the Company's Annual Information Form and in each Management's Discussion and Analysis. Forward-looking information may also be based on other various assumptions including, without limitation, the expectations and beliefs of management, the assumed long term price of copper, zinc, lead and nickel; that the Company can access financing, appropriate equipment and sufficient labour and that the political environment where the Company operates will continue to support the development and operation of mining projects. Should one or more of these risks and uncertainties materialize, or should underlying assumptions prove incorrect, actual results may vary materially from those described in the forward-looking statements. Accordingly, readers are advised not to place undue reliance on forward-looking statements.
Sophia Shane
Investor Relations North America
John Miniotis
Senior Manager, Corporate Development and Investor Relations
Robert Eriksson
Investor Relations Sweden
+46 8 545 015 50
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Water Heater Recirculating Pumps
Group Pushes Heat Pumps as Alternative to gas – Rutland Herald
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Ain't no molehill small enough …
President Obama's decision to restore the original Native American name to our continent's tallest mountain was met by an entirely expected chorus of wails from right-wingers and racists. Dead white guys need love, too, even if they'd never set foot in Alaska.
Personally, I was surprised that the mountain was still officially named for our 25th president.
The ink hadn't dried on the historic agreement between Western allies and Iran seeking to curb latter's nuclear weapons program when, predictably, it was denounced by saber-rattling right-wingers in Israel and Washington.
Whether the deal makes sense or not will be debated in the coming months. Obama plans to undertake a 60-day campaign to sell the accord to a skeptical public. His sales pitch will, of course, fall on the deaf ears of certain players (namely those mentioned earlier).
I, for one, believe it's time to give diplomacy a chance. War in that part of the world can have devastating and unpredictable consequences, a fact of which Americans should be well aware.
Still alive and kickin'
Republicans have tried six ways from Sunday to repeal, defund, hobble, gut and otherwise kill the Affordable Care Act, also known as Obamacare. King v. Burwell, decided yesterday by the U.S. Supreme Court in favor of the Obama administration, was likely the party's last, best hope in its pursuit of … what, really? Denying affordable health insurance coverage to 6.4 million Americans based on nothing more than a persnickety, legalistic definition of a six-word passage our of a nearly 5,000-page piece of legislation?
Whatever their motive, the GOP has failed and Obamacare lives on.
Never forget & never remember
Do far-right Republicans like Texas Rep. Louis Gohmert know when they're spouting nonsense? Really, who could be so dumb as to buy into a stupid notion like this?
Then, again, lots of people voted for this guy. And perhaps it's simply the case that Gohmert and the rest of the GOP establishment are hoping we all forget who was responsible for the current mess in the middle east.
Not taking the bait
The Trans-Pacific Parntership is the latest global-trade agreement to come down the pike, with supporters singing praises of its economic benefits and warning darkly of handing countries like China the upper hand should it somehow be rejected by Congress. Cloaked in a shroud of fast-track secrecy, the Obama administration is attempting to push the pact through without allowing anyone to review its details, outside of negotiators and the corporations that stand to benefit most.
Liberals and unions have seen this before. The unexpected monkey wrench that Democrats threw into the works earlier this week was a reminder of the human costs of globalization as well as a protest against the opacity surrounding the process. The left generally is fond of the president, but disinclined to take the bait on this issue.
Runaway train
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Riding the crazy
Texas is ripe with nuts, and elected GOP officials are ready to harvest 'em (or at least their votes).
One man's human-rights abuses …
President Obama's decision to normalize relations with the island nation of Cuba enjoys wide public support, even within the Cuban-American community. But Republicans — due in part to their inability to consider foreign relations in new ways and in part because this deal was cut by a president they loathe — are predictably beside themselves, citing Cuba's record of human rights abuses.
And about those human rights abuses … when are you guys going to allow Gitmo to close?
One flew over …
The preliminary details of a framework for a diplomatic deal regarding Iran's nuclear power program are emerging, much to the consternation of hawks in Israel and D.C. who tried mightily to strangle the effort in its crib. For the moment, at least, the accent is on a path to peace rather than war.


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WATCH: Marvel Releases "Daredevil" Season 2 Teaser
TV, Comic Books
Showing results 426-445 of 2237
The "X-Men" star is in talks to play a different kind of hero in director Francis Lawrence's adaptation of the Greek epic.
The Doctor Doom actor is set to join Tom Hiddleston, Brie Larson and Corey Hawkins in Legendary Pictures' King Kong origin story.
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Artist William Stout offers a look at illustrations he created in the 1990s for a "Jurassic Park" animated series that never made it off the ground.
Gentle Giant unveils two new jumbo figures -- one from its "Batman: The Animated Series" line, the other from "DC Super Powers."
Goonies may never say die, but the owner of a house in Astoria, Oregon, is saying goodbye to fans of the classic adventure comedy.
A Kickstarter campaign to fund an immersive (and official) theatrical experience based on "The Walking Dead" has rocketed past its goal.
When the legendary "Star wars" poster artist praises "The Force Awakens" as perhaps the best in the franchise, we may want to take notice.
Jim Gordon, Harvey Bullock, Bruce Wayne, Fish Mooney, Selina Kyle and Oswald Cobblepot are getting the Pop! vinyl treatment from Funko.
The polystone statue is based on the antagonist from the video game "Batman: Arkham Knight" is impressive, certainly, but so is the price: $900.
With the launch of "Disney Infinity 3.0" just 11 days away, Disney Interactive has released a new trailer for "Star Wars: Twilight of the Republic."
It may be difficult to settle on just which of Marvel's female characters is the most badass; luckily, there's now a handy infographic to help you decide.
New footage debuts in the latest trailer for the Ridley Scott drama.
Derek Connolly will take a last-minute pass the script for the Legendary Pictures film, which stars Tom Hiddleston, Brie Larson and Corey Hawkins.
The company has launched a $500,000 Kickstarter campaign to pay for upgrades to its 12,000-pound, 15-foot MegaBot Mark II.
The nominees have been announced for the 2015 Ignatz Awards, featuring a diverse lineup of creators that's more than half women.
Following the presidential candidate's Batman claim, the longtime voice of the Dark Knight has decided to set the record straight.
All Nippon Airways will follow the October debut of its R2-D2 aircraft with two more "Star Wars"-themed jets. However, there's no sign of Threepio just yet.
Scooby-Doo and the gang arrive in "LEGO Dimensions" with a trailer that blends cartoon footage with a ton of gameplay.
After inspiring a hashtag and a parody Twitter account, the next logical step in the evolution of the "BatTrump" meme is, of course, an animated mashup.


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Motel 6 Santa Fe
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Day 3 - Exploring the town of Santa Fe
A full day of sightseeing in downtown Santa Fe is ahead of us. After a nice breakfast at the AirBnB home's porch, we drove into the city, started in the Cathedral Basilica of Saint Francis of Assisi, where we joined the ongoing Sunday mass.
In front of the church is the statue of Kateri Tekakwitha (1656-1680), first Indian ...
... to try and unblock the sink still. Hayley found a plunger and I rather carelessly gave it a try as Alex was still under the sink, so I splashed him with the nasty brown sludge. Then Hayley came back and immediately did the same thing! Poor Alex.
We did eventually succeed in getting everything to drain away, and have decided to never touch the blasted thing again! I took another bath as I too got splashed with icky sink juice, and Hayley cooked Alex and herself ...
Caverns and UFOs
... basically implying that it was in fact a UFO that landed that the military tried to cover up. Needless to say I found it quite tacky and didn't scream scientific evidence but some people got really into it. Once we were finished at the museum we were off again with out destination of Santa Fe. This was probably one of if not the most boring drive of the trip so far. There was nothing to look at outside and the road was dead straight. We finally arrived in ...
Great day in Santa Fe!
... this chapel is the first Gothic structure west of the Mississippi.
Well, back to the story about the staircase. The carpenters built the most beautiful chapel and choir loft. One error – no stairway to link the two. And, since the loft was extremely high, there wasn't enough space to build a normal staircase. Carpenters were called in and no one could figure out how to build it. So, the nuns ...
Day 95 – Tuesday, July 2 2013
... the Museum of History. We learned more about the area and its history. There was also a large display of archeological work in the area. We learned that before any new construction is started, the archeologists have to examine the area for artifacts or graves of native people. If graves are found, efforts are made to determine the tribe of the deceased and return the bones to them. Pictures were not allowed so we can't share. ...


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Hot milf gets her juicy pussy fingered and licked, and gives some head to her hubby's nice thick cock before taking a big load of hot cum in her pussy
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not feeling the man-thong
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schoen hat er Sie abgefuellt.


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Sri Lanka
|Showing 1-20
Sri Lanka v England, 6th ODI, Pallekele
'Opening spots are still open' - Mathews
Dec 12, 2014: With Sri Lanka having been expected to wrap up the series handily, Angelo Mathews and the selectors had hoped to don their white coats and put World Cup hopefuls under the microscope in low-pressure matches towards the back end of the tour
England in Sri Lanka 2014-15
The six yet to cement their World Cup tickets
Nov 25, 2014: ESPNcricinfo selects six players with World Cup places to prove
Pakistan in Sri Lanka 2014
Chandimal, Mendis dropped for Pakistan Tests
Jul 31, 2014: Sri Lanka have left out wicketkeeper batsman Dinesh Chandimal and spinner Ajantha Mendis from their 15-man squad for the Pakistan Tests starting next week
South Africa in Sri Lanka 2014
Tharanga recalled for South Africa Tests
Jul 12, 2014: Sri Lanka opener Upul Tharanga, who last played a Test in 2007, has been recalled to the Test squad for the South Africa series
England v Sri Lanka, World T20, Group 1, Chittagong
Mendis - bird of prey or sitting duck?
Mar 27, 2014: Batsmen have often struggled to figure out Ajantha Mendis' bowling, but now it is time for Sri Lanka to figure out whether the spinner should be persisted with
Netherlands v SL, World T20, Group 1, Chittagong
Sri Lanka consign Netherlands to record low
Mar 24, 2014: Dinesh Chandimal chose to bowl against Netherlands, largely because he feared dew would set in later in the evening to hamper the bowlers
India v Sri Lanka, Asia Cup, Fatullah
Sangakkara ton gives Sri Lanka tense win
Feb 28, 2014: Kumar Sangakkara made an under-pressure century to steer Sri Lanka to their second straight win and to the brink of a place in the Asia Cup finals
Bangladesh v Sri Lanka, 2nd Test, Chittagong, 3rd day
Sri Lanka discover their limitations
Feb 6, 2014: As they spurned the opportunity to grab a lead in excess of 200 on the third day in Chittagong, Sri Lanka would have discovered some of their limitations in fielding and the lack of a bowling bench strength
Sri Lanka in Bangladesh 2013-14
Ajantha Mendis returns to Sri Lanka's Test squad
Jan 23, 2014: Ajantha Mendis has been named in Sri Lanka's Test squad to Bangladesh, after he had been directed to return from the Big Bash League at short notice this week
Sri Lanka news
Malinga recalled from BBL over 'fitness issue'
Jan 20, 2014: Sri Lanka Cricket has recalled fast bowler Lasith Malinga and spinner Ajantha Mendis from the Big Bash League with immediate effect
Pakistan v Sri Lanka, 1st ODI, Sharjah
Top order in focus for both teams
Dec 17, 2013: Sri Lanka's ability to tackle spin and Pakistan's progress against the new ball might play the biggest part in determining the outcome of the first ODI in Sharjah
Big Bash League 2013-14
Mendis signs for Sydney Thunder
Dec 6, 2013: Ajantha Mendis, the Sri Lanka spinner, has signed with Sydney Thunder for the upcoming season of Big Bash League
Premier Limited overs tournament
Moors tie with Tamil Union on rain-hit opening day
Dec 1, 2013: A round-up of all the matches played in the Premier Limited Overs Tournament on December 1, 2013
West Indies tri-series
Injured Dilshan out of West Indies tri-series
Jun 21, 2013: Upul Tharanga will replace the injured Tillakaratne Dilshan in Sri Lanka's squad for the tri-series in West Indies, while Ajantha Mendis also gets a recall at Thisara Perera's expense
Premier League Tournament 2012-13
Ajantha Mendis takes 11 in innings win for Army
Feb 18, 2013: A round-up of the third round matches of the Premier League Tournament 2012-13
Bangladesh in Sri Lanka 2012-13
Mathews takes over as Sri Lanka captain
Feb 14, 2013: Angelo Mathews will take over as Test and ODI captain from Mahela Jayawardene for the home Test series against Bangladesh. Dinesh Chandimal will be Mathews' deputy but will lead the national side in the Twenty20 format
Australia v Sri Lanka, 2nd ODI, Adelaide
Hungry hosts chase follow-up victory
Jan 12, 2013: ESPNcricinfo's preview of the second ODI between Australia and Sri Lanka in Adelaide
Sri Lanka in Australia 2012-13
Ajantha Mendis returns for limited-overs series
Jan 2, 2013: Sri Lanka have picked Kushal Perera, the wicketkeeper-batsman, for the limited-overs series in Australia, and spinner Ajantha Mendis makes a comeback after suffering a side strain during the World Twenty20 late last year
New Zealand in Sri Lanka 2012-13
Ajantha Mendis out of limited-overs squad
Oct 25, 2012: Ajantha Mendis, the spinner who has been struggling with a side strain, has been left out of the Sri Lanka squad for the Twenty20 and the first three ODIs against New Zealand
New Zealand in Sri Lanka 2012
Sri Lanka hope for Ajantha Mendis' recovery
Oct 21, 2012: Ajantha Mendis' fitness remains a concern for Sri Lanka as they prepare to take on New Zealand
|Showing 1-20
What you need to know about the Cairns perjury trial
Background to the Chris Cairns perjury case and what to expect over the coming weeks
'Show some respect to your players'
The cricket world reacts to the incidents of bottle-throwing during the second India-South Africa T20 in Cuttack that caused two interruptions in play
The makeshift opener who batted and batted
Thrust into the job in Kanpur in 2004, Andrew Hall gave an underachieving South Africa side belief that they could wear India down at home
Who is the better bowler in challenging conditions?
The Azhar Ali answer to Pakistan's top-order woes
In Pakistan's Test history, no player batting in the top three positions has scored 4500 runs; Azhar Ali is well on course to becoming the first
Who is the better bowler in challenging conditions?
He's delightful to watch because he makes batting look easy, but there are some gaps in his technique in the long form
Hard to make 'em like Thommo and Typhoon (37)
Though the game has had many quality fast bowlers, none have been quite as lethal as Jeff Thomson and Frank Tyson
'Show some respect to your players' (35)
The cricket world reacts to the incidents of bottle-throwing during the second India-South Africa T20 in Cuttack that caused two interruptions in play
Ranji Trophy's familiar tale of indifference (24)
The Ranji Trophy is a logistical wonder, yet it exists in a vacuum at the heart of the Indian cricket season


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As we informed you last week, the Open Payments review, dispute and correction process started today and will continue through September 11, 2014. This is the process that allows physicians and teaching hospitals to review and dispute information regarding payments and other transfers of value that pharmaceutical, biotechnology and medical device manufacturers and group purchasing organizations (GPOs) recently reported to CMS, before CMS publicly discloses this information by September 30, 2014.
To assist with this process, CMS released an updated Open Payments User Guide to provide a comprehensive overview of the Open Payments system and requirements. CMS also released the following Quick Reference Guides:
Physicians: How to Register in the Open Payments system
CMS further announced today that a National Provider Call regarding the registration, review, and dispute processes will be held on Tuesday, July 22, 2014, from 2:30 p.m. – 4:00 p.m. Eastern. The target audience is physicians, teaching hospitals, professional organizations, physician staff, and other interested parties. Registration for this teleconference is available here.
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DISCLAIMER: Because of the generality of this update, the information provided herein may not be applicable in all situations and should not be acted upon without specific legal advice based on particular situations.
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|Smith & Loveless - Above All Others™
Smith & Loveless offers a diverse array of water and wastewater treatment and pumping system solutions for the world's water problems. Additionally, we deliver unique technologies and consumables for mineral processing. Please browse our many solutions to learn more about our product offerings.
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Frank J. Rebori, president of Smith & Loveless Inc., and chairman of the Water and Wastewater Equipment Manufacturers Association (WWEMA) wants everyone to turn off the tap, and know what it means to go without water… for a period time.
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Events Calendar
Smith & Loveless conducts numerous seminars, Pump Schools, trade show exhibits and other events throughout the year. For more details, contact Marketing Communications Manager, Darby Ritter.
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Mysticism in the world's religions
Oxford University Press, 1976 - Mysticism - 210 pages
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"Mysticism in the World's Religions" is one of the books in the primary bibliography of my free ebook on comparative mysticism. "The greatest achievement in life" at suprarational.org/gail2012.pdf has ... Read full review
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Ichiban restaurant is now Hibachi Buffet in Rockford after its owner renovated the space to attract more customers.
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Even though some of the very old KBs, which now refer to unsupported products, state that taking databases offline to run periodic offline defragmentation with ESEUTIL is not recommended some folks in the field still want to do this.
Previously when there was only a single copy of a database, running offline defragmentation would cause minimal impact, apart from the time required to do the defragmentation process which could be several hours or longer depending on database size and disk throughput. This changes when we consider having multiple copies of a database in a Database Availability Group (DAG).
So you may be wondering how best to defragment Exchange 2010 databases that are in a DAG as people often look at the white space in a database and seek to immediately reclaim it.
In short, this is not a good idea for a couple of reasons:
Please note that we are discussing offline defragmentation via ESEUTIL /D, and not online maintenance routines that now run 24 * 7 in newer versions of Exchange and in online maintenance windows in previous versions.
What happens when an Exchange database is defragmented using ESEUTIL /D? The defragmentation process will copy out valid pages of ESE data from the old database file to a new database. This process leaves white space behind as it does not contain data. You will note that I specifically said new database. This has a different GUID than the original database. Creating a database with the same name, but different GUID, means that Exchange sees them as different databases not as multiple copies of the same database.
This will result in errors like the following since the databases are not copies of one another. Errors that may be seen include, but are not limited to:
Let's look at an example of the impact caused by running offline defrag against a database that is replicated in a DAG.
We shall defragment database, DB01. Our starting configuration has two copies of this database and all is currently running well.
So let's dismount DB01, and then validate that the two mailbox servers have the same GUID for DB01. We are using ESEUTIL /MH to dump out the header from the database.
On the first mailbox server we see the Rand of 2733649. The GUID is displayed in the 'DB Signature' line and is the 'Rand' value. Be sure to look at the correct signature as there is a signature for both logs and databases. It is expected that the Rand in these two lines will be different.
On the second mailbox server we see the same Rand of 2733649, you can see the server name in the title bar of the PowerShell window.
We have shown that the same database is present on both servers, i.e. both copies have the same Rand of 2733649.
Let's now defragment DB01 on the first server, then see what happens……
Then let's check the Rand to see if the old value of 2733649 is still present:
Nope, It's not. The Rand is now 143007541. That shows that this is a different database. Same name, but this is a different database.
Trying to activate the database copy on another server will create a sea of red in the application event log. You will receive the errors listed above, and the most descriptive is Event ID 4807:
At this point since the databases are no longer copies of one another we will have to re-seed the copy of the database. Depending upon database size, disk throughput and network capacity this can take an extended period of time. Let's use PowerShell to re-seed the database copy:
Update-MailboxDatabaseCopy –DeleteExistingFiles –Identity DB01\Consea-MB2
This will have to be repeated for all database copies of the database in question. If there are multiple copies over a WAN link then it would be a good idea to manually specify the seeding source using the –SourceServer switch. That way one copy can be seeded over the WAN, and other copies can then use that as a local source, thereby minimising WAN traffic and decreasing time.
Note that there are multiple options worth checking out with Update-MailboxDatabaseCopy. They include options to explicitly choose a network, encryption and compression. Chances are if you used Exchange 2010 RTM then you are quite adroit at using the –CatalogOnly switch!
When the seeding task completes, we can check that the database copies are OK
Checking the Rand on the updated copy of the database, we can see that it has been updated and now has the same Rand which was generated by the defrag, 143007541.
Having to take a database offline for hours to defragment, and then manually reseeding all of its database copies is pretty painful. Is there a better way to do this?
There certainly is!
Since Exchange 2010 introduced the online mailbox move feature, it is now pretty seamless to perform mailbox moves to a new mailbox database and when the old database is empty, simply delete it! This process can be made even better with use of the SuspendWhenReadyToComplete parameter. As an example:
New-MoveRequest -Identity 'User-21' -TargetDatabase DB01 –SuspendWhenReadyToComplete
This copies the vast majority of the mailbox content and then pauses. The administrator will manually resume the move request using Resume-MoveRequest. So this means we can copy mailbox content through the day with no user impact. After hours the suspended move can then be rapidly completed. This has to be one of my favourite Exchange 2010/2013 features!
The same logic can also be applied to a mailbox database that must be evacuated for other reasons. This may be necessary if file system AV has scanned the database as it will be in an unknown and thus unsupported state.
Note that the Mailbox Replication Service (MRS) is throttled, and if you wish to apply a little accelerando to the move process then you will need to take a look at the throttling configuration.
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Rhod, great article as always. In a follow-up article I would love to see comments as to how long a db defrag can take and why trying to reclaim white space is often a fool's errand. Cheers and take care...sc
Howdy Sean!
Very true, I can't recall when I last did an offline defrag to reclaim space. Last time would have been many, many moons ago!
Say hello to Mr Thiessen for me please :)
Got a small query here regarding seeding...
Consider 2(P) + 1(DR) copies of avg DB size 500GB.
Copy/Replay status seems to be normal i.e. 0. Suddenly something went wrong like log file got missed, ending up in disk space issue(P).
Action plan - you now you cannot either activate nor truncate the logs.
Dismounted the DB(P Active) moved the logs because it was healthy & mounted.
Q - having the passive copy node *.edb file(500GB) can we use this as incremental updating the DB instead of complete DB seeding between nodes in P & DR site.
I tried with –SeedingPostponed / -Force switch but no luck - does it works really...or there is no other option but to complete reseed...?
Hi Charles,
Missed this over the long holiday weekend.
Did you do what I have above and dump out the databases headers with ESEUTIL /MH ?
What I expect you to find is that on the active instance, the database is updated to use the new log stream but on the passive that copy of the database file is still looking for the original log stream GUID. Since it does not match the passive DB will not attach to the log stream.
Does that match your observed behaviour ?
So you are right about the new log stream as it will not attach but is there any way we could use the same passive old DB for incremental seeding instead asking system to deleteexistingfiles and seed.
Don't think so but, let me have a think about that please. Things are a bit hectic at the mo, and I'll reply when I get a chance.
Hmn I understand - Not able to figure out any workaround but will hope anything to come up in future may be seeding via WAN to DR wouldn't be feasible for DB size from 500GB-2TB
Hi, I had a database with several hundred users, moved them to another database but the original db with no users is still 195GB after 2 months, so how do I reduce the size of this if you do not recommend a defrag?
Nick - just remove the database. Create another DB if needed.
Why would you want to defrag it?
Landing on your blog searching for this answer: If you move a mailbox database, this is a pure file copy? No database defrag (maybe on NTFS level :)) is done with a move of the database?
Correct - the only way to defrag the Exchange database to reclaim whitespace is to use eseutil when the DB is offline.
Moving the file around does not do that.
I would also like to second your statement that the –SuspendWhenReadyToComplete switch used on Local Moves could be used for the transitions between Exchange versions also.
I had built an automated script that runs each night after hours that will 'Complete Move Requests' so i could start a group of moves during the day, and allow the system to complete them after nobody was using the system any longer. (this also allows for the
hour+ that MS states AD needs to update before the users belonging to the mailboxes are able to utilize their moved mailboxes again)
This saved me countless hours of my own personal time during the migration, and with small maintenance tasks since then as the Move Request resume does not require manual intervention (above setting up a scheduled task to run daily after all client use of the
system is finished and before backup jobs begin).
This is the command I used, just in case anyone else finds it helpful.
Get-MoveRequest -MoveStatus autosuspended | Resume-MoveRequest
That is certainly one of my favourite Exchange 2010 features Altren - saves so much time for us and minimises end user impact.


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Member since: aug 3, 2010
Last login: feb 28, 2011
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New Churnalism web site and browser extensions. Check articles for press release churn while you browse!
Sue Crawford
Latest 5 articles
Whatever happened to…? Heartbeat's Tricia Penroseexpress.co.uk, Saturday 19 September
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Corrie's Lucy Fallon: I'd run a mile from bad boysexpress.co.uk, Saturday 19 September
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Revenge is sweet... Interview with Gaynor Fayeexpress.co.uk, Saturday 15 August
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Corrie's Sally Dynevor: The bravest thing I've ever done is jump out of an aeroplaneexpress.co.uk, Sunday 2 August
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Actress Emily Watson on drama about the 7/7 bombings: This felt like a call to dutyexpress.co.uk, Saturday 4 July
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Sue Crawford's published articles – last 12 months*
144 articles since December 2007 with an average of 946 words
- 144 articles (since December 2007)
- Average article: 32 column inches (946 words)
- Shortest article: 1 column inches (20 words)
- Longest article: 83 column inches (2484 words)
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*Please note that these statistics are based only on articles published online in these publications.
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Anime: The Cursed Holy Sword
The Fifth One Piece movie, mainly focused on Zoro.On Asuka Island, the Straw Hat Pirates learn about the Seven Star Sword, considered the world's most beautiful sword and a valuable treasure, though it contains a deadly curse. When they come back to the ship, they find that Zoro, who was to act as guard, is missing, but they are forced to leave port when they are attacked by Marines. They dock in another section of the island and come across a village, whose job it is to keep the Seven Stars Sword sealed. It is soon attacked by Marines who are trying to free the swordamong whom is Zoro! What reason could Zoro have for doing this? What is the secret of the sword? Why would Marines want it? The answers lie on the night of the red moon.
This movie contains examples of:
This movie contains examples of:
- Armor Is Useless: Averted by Bismarck's full plate armor. Too bad it doesn't cover the face or the top of the head.
- Barrier Maiden: Maya, being the descendant of the original maiden's bloodline, can summon a magical barrier by praying the Gods of Asuka, allowing her to suppress the power of the Sword. This ends up being mildly effective, in that while the Sword is too powerful to be stopped by the barrier, her prayers still weaken Saga, allowing Luffy to clobber him.
- Big Bad: Saga, though to be honest, the Seven Star Sword might as well be the Bigger Bad.
- Brainwashed and Crazy: Saga and anyone afflicted by the Sword. Mostly seen with the Marines. The only one who's trying to fight back the influence is Toma.
- Brick Joke: Near the beginning, the Merry is propelled in the air and land on a hippo-rhino hybrid, causing him to spit a fish that was choking him and making him fell in love with the ship. Much later, as the Strawhats leave Asuka Island, the very same hippo-rhino appears, trying to court the Going Merry.
- Cool Sword: The Seven Star Sword, likely a nod to a weapon with the same name appearing in Journey to the West.
- Curb-Stomp Battle: All of Saga's servants are defeated in a jiff. Saga is on the receiving end during his One-Winged Angel when Maya starts praying, weakening the sword.
- Darker and Edgier: Compared to the previous movies it has a darker, more serious atmosphere, though not nearly as dark as the following film which was the darkest film in the series so far.
- Dissonant Serenity: Touma, Until the climax, that's it.
- Evil Weapon: The Seven Star Sword. Which is rather odd, as One Piece usually has an explanation (most of the time: Devil Fruit) for these kind of things.
- Face-Heel Turn: Seemingly Zoro, before his real motives are revealed.
- Family-Unfriendly Violence: When Saga is possessed by the Sword in the beginning he doesn't simply cut down the pirates attacking him and Maya, he literally bisects them.
- Fat Bastard: Boo Kong, so fat that he's actually spherical and can bounce around like a ball, crushing people and buildings under his own weight.
- Foreshadowing: Early on, Saga himself explains that nor he nor his men can get too close to the Sacred Orbs. Later Nami takes advantage of this detail to defeat a whole group of possessed Marines by keeping them at bay with the Orb.
- Guest Star Party Member: Lacos, one of the villagers, joins Sanji as he goes to put the Orb in its tower. He doesn't do much though, except fighting mooks.
- MacGuffin: The Three Sacred Orbs that are necessary to seal the power of the Seven Star Sword. Oddly, Saga's henchmen can't touch them, and not even get close to them, forcing Saga to ask for Zoro's help.
- The Magic Goes Away: In the end, both the Sword and the Orbs are gone for good.
- One-Handed Zweihänder: Invoked, when Luffy, upon seeing the Seven Star Sword hanging from Saga's back, asks him if he can really use such a long sword with only one hand. He can, btw.
- One-Winged Angel: Saga. First he gets his arm back and turns into a hulking brute with crazy eyes whose powers, however, are hindered by Maya's prayers.. Then, the sword merge with him, making him expressionless and deadlier.
- Playing with Fire: Saga, thanks to the Sword. It looks like some sort of wisp though.
- Shaping Your Attacks: One of them takes the form of a serpent made of cold flames that bites the victim.
- Razor Wind: Saga again.
- Red Eyes, Take Warning: All those under the influence of the Sword sports these.
- Running Gag: Maya's Grandmother seems very short, but when she's surpised she suddenly springs up to great height, freaking everyone out.
- Shout-Out: The Seven Star Sword may be a nod to the weapon with the same name from Journey to the West.
- Single-Stroke Battle: How Zoro deals with Saga in the end. Though the latter survives.
- Voice of the Legion: Saga, under the influence of the Sword.
- You Gotta Have Blue Hair: Maya, Saga's girlfriend.


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Special Culture Techniques
Many microbes have special growth conditions or require precautions to grow in a laboratory setting, leading to special culture techniques.
Learning Objective
Evaluate special culture techniques
Key Points
- Microbes, often those that we know little about, have to be cultured with undefined media or growth conditions.
- The use of animals to culture animals is sometimes necessary as no simple media can be used, this presents technical and ethical issues.
- As human pathogens are often studied by microbiologists, special safety conditions know as biosafety levels are used to keep researches free of infection from the pathogens they study.
Lyme disease
Infection by a bacterium of the genus Borrelia which is transmitted by ticks. Symptoms include a rash followed by fever, joint pain, and headaches.
yellow fever
An acute febrile illness of tropical regions, caused by a flavivirus and spread by mosquitoes, characterized by jaundice, black vomit, and the absence of urination.
Full Text
Microbiologists would prefer to use well-defined media to grow a microbe, making the microbe easier to control. However, microbes are incredibly varied in what they use as a food source, the environments they live in, and the danger levels they may have for humans and other organisms they may compete with. Therefore they need special nutrient and growth environments. To grow these difficult microbes, microbiologists often turn to undefined media which is chosen based on price and more-so in this case by necessity as some microorganisms have never been cultured on defined media. Some special culture conditions are relatively simple as demonstrated by microaerophile.
A microaerophile is a microorganism that requires oxygen to survive, but requires environments containing lower levels of oxygen than are present in the atmosphere (~20% concentration). Many microphiles are also capnophiles, as they require an elevated concentration of carbon dioxide. In the laboratory they can be easily cultivated in a candle jar. A candle jar is a container into which a lit candle is introduced before sealing the container's airtight lid . The candle's flame burns until extinguished by oxygen deprivation, which creates a carbon dioxide-rich, oxygen-poor atmosphere in the jar. Many labs also have access directly to carbon dioxide and can add the desired carbon dioxide levels directly to incubators where they want to grow microaerophiles.
Candle jar
A candle is lit in a jar with a culture plate. The lid is put on, as the burns it increases the carbon dioxide levels in the jar.
Animals can often be used to culture microbes. For example, armadillos are often used in the study of leprosy. They are particularly susceptible due to their unusually low body temperature, which is hospitable to the leprosy bacterium, Mycobacterium leprae. The leprosy bacterium is difficult to culture and armadillos have a body temperature of 34°C, similar to human skin. Likewise, humans can acquire a leprosy infection from armadillos by handling them or consuming armadillo meat. Additionally, Syphillis which is caused by the bacteria Treponema pallidum is difficult to grow with defined media, so rabbits are used to culture Treponema pallidum. Treponema pallidum belongs to the Spirochaetesphylum of bacteria.
To date Spirochaetes are very difficult if not impossible to rear in a controlled laboratory environment. This also includes other human pathogens like the bacterium that causes Lyme disease. Using animals to culture human-pathogens has problems. First, the use of animals is always difficult for technical and ethical reasons. Also, a microbe growing on animal other than a human may behave very differently from how that same microbe will behave on a human. Some human pathogens are grown directly on cells cultured from humans. Exemplified by the bacteria Chlamydia trachomatis, the bacteria responsible for the sexually transmitted infection (STI) in humans known as Chlamydia. As Chlamydia trachomatis only grows in humans. The human cell culture known as McCoy cell culture is used to culture this bacteria .
Chlamydias bacteria group
Light microscope view of cells infected with chlamydiae as shown by the brown inclusion bodies.
A large concern of microbiology is trying to find ways in which humans can avoid or get rid of microbrial infections. As typified by some of the above examples, some microbes have to be grown in the lab, and some of them can infect humans. To deal with this, microbiologists use a classification of biosafety levels. A biosafety level is the level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). In the United States, the Centers for Disease Control and Prevention (CDC) have specified these levels.
Biosafety Level 1: This level is suitable for work involving well-characterized agents not known to consistently cause disease in healthy adult humans, with minimal potential hazard to laboratory personnel and the environment.
Biosafety Level 2: This level is similar to Biosafety Level 1 and is suitable for work involving agents of moderate potential hazard to personnel and the environment. It includes various bacteria and viruses that cause only mild disease to humans or are difficult to contract via aerosol in a lab setting such as chlamydia.
Biosafety Level 3: This level is applicable to clinical, diagnostic, teaching, research, or production facilities in which work is done with indigenous or exotic agents that may cause serious or potentially lethal disease after inhalation. It includes various bacteria, parasites, and viruses that can cause severe to fatal disease in humans, but for which treatments exist (eg. yellow fever).
Biosafety Level 4: This level is reserved for work with dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections, agents that cause severe to fatal disease in humans for which vaccines or other treatments are not available, such as Bolivian and Argentine hemorrhagic fevers, Marburg virus, and the Ebola virus. Very few laboratories are biosafety level 4 .
Positive pressure suit
A scientist puts on a positive pressure suit, something needed to work with the most dangerous human pathogens in a biosafety level 4 laboratory.
Key Term Reference
- Parasite
- Appears in these related concepts: Double-Stranded RNA Viruses: Retroviruses, Cyclospora Diarrheal Infection, and Antiprotozoan and Antihelminthic Drugs
- bacteria
- Appears in these related concepts: The Species Concept in Microbiology, Shared Features of Bacteria and Archaea, and Chromosomes and DNA Replication in the Archaea
- bacterium
- Appears in these related concepts: Overview of Gram-Positive Bacteria and Actinobacteria, Mu: A Double-Stranded Transposable DNA Bacteriophage, and Generation Time
- capnophile
- Appears in this related concept: Gas Requirements
- cell culture
- Appears in these related concepts: Complex and Synthetic Media, Reporter Fusions, and Tissue Culture of Animal Viruses
- classification
- Appears in these related concepts: Classification and Identification of Helminths, Maximize Understanding, and History of Microbiology: Hooke, van Leeuwenhoek, and Cohn
- culture
- Appears in these related concepts: Cultural Values, Context of Culture and Gender, and Minimal Inhibitory Concentration (MIC)
- hemorrhagic
- Appears in these related concepts: Cystitis, Classic Viral Hemorrhagic Fevers, and Safety in the Microbiology Laboratory
- infection
- Appears in these related concepts: Bacterial Pneumonias, Antibiotic Discovery, and Infection
- inhalation
- Appears in these related concepts: Scarlet Fever, Blastomycosis, and Coccidiomycosis
- leprosy
- Appears in these related concepts: Koch and Pure Culture, Antimycobacterial Antibiotics, and Leprosy
- microbes
- Appears in these related concepts: Dendritic Cells, Biofilms, Persisters, and Antibiotic Tolerance, and Factors that Affect Germicidal Activity of Chemicals
- microorganism
- Appears in these related concepts: Synthetic Antimicrobial Drugs, Normal Microbiota and Host Relationships, and Opportunistic Microorganisms
- microorganisms
- Appears in these related concepts: Putting Foreign DNA into Cells, Food Spoilage by Microbes, and Fermented Foods
- mycobacterium
- Appears in these related concepts: Effective Disinfection, Ecological Associations Among Microorganisms, and Actinobacteria (High G + C Gram-Positive Bacteria)
- organisms
- Appears in these related concepts: Viral Genomes, Natural Active Immunity, and Planktonic Communities
- pathogen
- Appears in these related concepts: Nongenetic Categories for Medicine and Ecology, Types of WBCs, and White Blood Cells
- pathogens
- Appears in these related concepts: Gammaproteobacteria, Technology and New Infectious Agents, and Interferons
- susceptible
- Appears in these related concepts: The Stringent Response, Other Fungi Involved in Respiratory Disease, and Chain of Transmission
- vaccine
- Appears in these related concepts: Development of New Vaccines, Genetically Engineered Vaccines, and Overcoming Density-Dependent Regulation
- virus
- Appears in these related concepts: Positive-Strand RNA Viruses of Animals, Viral Replication and Gene Expression, and Discovery and Detection of Viruses
Boundless vets and curates high-quality, openly licensed content from around the Internet. This particular resource used the following sources:
Cite This Source
Source: Boundless. "Special Culture Techniques." Boundless Microbiology. Boundless, 21 Jul. 2015. Retrieved 05 Oct. 2015 from https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/culturing-microorganisms-6/culturing-bacteria-58/special-culture-techniques-368-7658/


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Unless I misunderstood, you are against the creation of a macro forum ... because you think a person should look for the answers before asking for help. Either you assume people that are asking questions are not making any efforts on their own or you believe having a forum in which to ask the questions will prevent them from making any effort on their own.Originally Posted by Dan Fromm
FWIW - I didn't get much sleep last night and I failed to eat breakfast this morning. I'm not sure how I have managed to sit upright all day.
It's a nice theory but not everyone is wired the same and not everyone will become proficient at everything they practice. My mother cooked for many years but is still not a very good cook. My mother's cooking is only one example of many.Searching and failing to find or understand the answer is better than giving up in advance. I do a fair amount of searching, turn up surprises. Searching, in books and over the internet alike, is a learned skill. As with all learned skills, practice builds proficiency.
Do I just shoot film until I get what I think I want? No. You (I) need a foundation in which to work from, to experiment with. If something doesn't work I don't just throw in the towel and look for someone to solve it for me. I double check my foundational information and see if perhaps I missed something or misunderstood something. I try again. If my results are the same then I start looking for answers. If my results are different from the first time then I compare the results and how I acheived them and try again. After a number of tries if I still cannot get it right I start looking for answers. This is an over-simplified answer but I don't have all night to write a book how I approach a given endeavor.So in fact you look for answers. That's great. Do you also try to understand what's going on and solve problems yourself before looking?


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She picked a plum today
30 November 2011 - 12:13am | quietword333
She took a bite, the juice dripped down her fingers and reminded her of god...
Golden ripple sun beam created a sparrow of worship
Bright dazzle specks of razor wings
Flash, whip upward, upward
and land again
salt of the wind no match for a hungry bird
who placed the pit in a black beak
The girl watched the sweet aroma fly away
and knew it was all part of the plan
the tear drop wasn't to weep
just a clear signal of water, earth, sparrow
where gods reside
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