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Favstar gets even better if you sign in.
Gift @rwaldron Favstar Pro to unlock their best tweet!Gift them Pro!
The ES6 screencast by @hendrikswan is absolutely awesome: http://tagtree.tv/ecmascript-6-episode-1 … #javascript
Excited to announce the new Johnny-Five website: http://johnny-five.io @nodebots #javascript #robotics
Project: fork @atomeditor, port to ES6 JS. Who's with me?
Barbie Patches a Critical Bug https://gist.github.com/rwaldron/88298af43f304c1e1436 …
First episode in my own #feministhackerbarbie series. <3
Object.assign has been updated to support a variable number of sources in ES6. Pass it on. #javascript
My first act as maintainer of JSHint: ban comma first, no option.
Just kidding.
Or am I?
I am.
My fellow computer scientists, technologists, engineers and hackers: let's get involved with http://www.fatalencounters.org (at very least RT)
ES6 Proxies http://tagtree.tv/es6-proxies Another excellent screencast by @hendrikswan & @tagtreetv! #javascript
RT @jamiekilstein: Hey new followers and old. You should all read what is happening in #ferguson as well. Militarized police going after a …
Dearest @github, I would so ❤ to get notified when a contributor updates (pushes new or amended commits) an open PR :)
Real-time face detection using OpenCV, Node.js, and WebSockets on Intel Edison https://www.youtube.com/watch?v=nVDL2-bFT3Y … http://drejkim.com/blog/2014/12/02/real-time-face-detection-using-opencv-nodejs-and-websockets/ … by @drejkim
Tessel 2 looks totally awesome. No more Lua runtime: REAL node.js/io.js FTW <3
So excited, already pre-ordered. https://tessel.io/
"Girls and Software" the most important thing you will read today (and reread everyday from now on) http://linuxjournal.com/content/girls-and-software …
Make: JavaScript Robotics (the book!) http://shop.oreilly.com/product/mobile/0636920031390.do … #nodebots
ECMAScript 2015 Internationalization API Specification (ECMA-402, 2nd Ed.) is officially an approved Ecma standard: http://www.ecma-international.org/news/index.html
This is me and @brianloveswords, taken during his technical interview @bocoup pic.twitter.com/bmxsPGKd4k
Everyone is pissy pants because they wanted a fight for equality but all they got was some bullshit workplace drama.
Message: white kids riot with impunity. "@bipartisansport ... #Ferguson pic.twitter.com/pmmAgXXhku"
Champion for all NodeBots. Creator of Johnny-Five, the JavaScript Robotics Programming framework for Node.js http://johnny-five.io
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"A market is the combined behavior of thousands of people responding to information, misinformation and whim."
Related quotes
"Development of a society depends on thought behaviour of their citizens! come thinking to over-power other, other has no unity but behead helpless cockroaches, some made plans to bribe today, some expecting someone is going to come and change everything, some thinking what is the next movie release,some planning what alcohol drink do we buy now!. Any-case it must be true that Society is an outcome of the thoughts of majority of their people. - Devaski"
"Treat a man as he is, and he will remain as he is. Treat a man as he could be, and he will become what he should be."
"Talk to every woman as if you loved her, and to every man as if he bored you, and at the end of your first season you will have the reputation of possessing the most perfect social tact"

subset: commoncrawl


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Grecotel Plaza Spa Apartments
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Crete palace of Knossos
Today we had an early start . We docked at Chania and very quickly boarded our bus to The Palace of Knossos, the home of the legendary Minotaur. Crete is 300 km wide (East to West ) at the widest part and north to South 33km with it narrows section being 12 kilometres. It has a population of 756,000 and tourism makes up 50 % of the islands economy . Chania is were Zorba The Greek (last nights film was shoot) Crete has one big valley in the centre with lots of mountains (the ...
Day 8 Kyle's birthday at Elofonisi beach
... goats, and sheep. As soon as Elofonisi came into view, we knew that the drive had been worth it. The beach has several inlets of turquoise water, set off by the white and pink sand. This part of the island can have very strong winds, so we headed to the more protected side of the beach and found ourselves some 3.5 euro lounge chairs under tiki style umbrellas. From there on, the day slipped lazily by as we alternated people watching, gazing out at e ...
Day 7 Chania Crete
We awoke late this morning still a little tired after our late arrival last night. I was just able to sync yesterday's post. Our hotel is an old Venetian building that has been beautifully renovated. We had the best yogurt I've ever had along with a huge variety of other items for breakfast in the charming courtyard. After breakfast we walked along the waterfront of the old Venetian harbor out to the light house to admire the ...
Chania and Samaria gorge
... war cemetery. Not sure how he would have defended the bay. Don't think it was a wise decision on his part. Anyway the Germans were never going to land there so guess he was saved the embarrassment of having to defend it. Cemetery closed due to spraying. Not a good day for seeing what I had planned to see. I did manage to go around the back of the cemetery via the beach and have a look though so all ...
July 1
... phone and tried to Google my way out (reception this high up is actually workable). I couldn't find a trail map, but someone did write something to the effect of "the trail goes up, then down into the plateau, then back up again." Fair enough, down I go.
I realized fairly quickly, though not quickly enough, that, if there is a path, that wasn't it. It was an unmanicured, treacherous, "swing the messenger bag around and climb down" affair. ...


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The Top 10 In Action Sports
01Kenton Cool And The Legend Of Everest
02The Martyn Ashton Interview: Meet the Man Who Got Back on the Bike
03Legends Behind the Lens: The Dan Milner Interview
04We Asked Surf Star Coco Ho For Her Step-by-Step Guide to the Basics
05The Tony Hawk Interview: How Is The Birdman Still Flying High After All These Years?
06We Toured Britain's Action Sports Hotspots With The New Ford S-MAX
07The Legend Of El Dorado: One Man's Obsession With Finding A City Made Of Gold
08I Am Legend: How Audiences – Real Or GoPro'ed – Can Affect Your Mind And Performance
09Watch A Freerunning King Defy The Laws Of Gravity In This Urban Assault On London
10Living On The Blood and Urine of Dead Sea Turtles: The Shocking Story of A Sailor Stuck in Hell
BambooSk8 BBQ Fall 2010
A Skateboarding video by bamboosk8 - 3,770 views
Another BBQ thrown at the BambooSk8 headquarters. The bar was set high when a team game of skate went down as Mostic Inc was there to giveaway prizes. Burgers, babes, and bangers....check it out!!!
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JC2D3 years ago Demo 1 comment
A small little demo of my idea for a 2D game based on DeusEx world... Two level of intense action! :)
jc2d demo
This group has been formed for the fans of the Deus Ex Trilogy and for the players was waiting for one the most anticipated game of the years: Human revolution.
No downloads were found matching the criteria specified. We suggest you try the download list with no filter applied, to browse all available. Post download and help us achieve our mission of showcasing the best content from all developers.


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Accused rapist/murderer Richard Hooten faces death penalty if found guilty
Southern Indiana man confessed to killing Clarksville teen Tara Willenborg
4:56 AM, Mar. 22, 2013
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Accused rapist/murderer Richard Hooten faces death penalty if found guilty
The Clark County, Ind., prosecutor announced Thursday that he will seek the death penalty against Richard Hooten in the murder and rape of a 17-year-old Clarksville girl early this month.
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Bizanet - FLA025DV
from £48 /night help Price for guests, Nights approx:
from £48 /night help Price for guests, Nights approx:
Estimated nightly price based on a weekly stay. Contact the advertiser to confirm the total cost.
Villa / 3 bedrooms / sleeps 6 Home 1723174
Availability Your dates are available
Villa / 3 bedrooms / sleeps 6
|Key Info
This charming holiday property offers endless vineyard views from most rooms. Adjoining the owner's home, it was previously a bed and breakfast g??te and all the bedrooms benefit from en-suite facilities. It is a perfect touring base from which to explore the region's numerous Cathar castles, to discover some beautiful market villages such as Lagrasse, to wander around the sun drenched historical towns of Perpignan and B??ziers, or the world famous medieval fortified city of Carcassonne. Laze on the endless miles of sandy beaches of the welcoming family resorts which begin at just 30km, dining al fresco under the pergola on returning to the property, with a glass of the local wine.FeaturesSemi-Detached | Brick/stone house | Electric heating | Shared plot | Parking on the plot(1 spaces) | Barbecue | Refrigerator | Freezer Box | Microwave | Washing machine | Dishwasher | 1 TV | CD player | 1 DVD player | Shower(3 count) | Toilet: Hot and cold water | Consumption costs excl. | View over the landscape | No | Houseowner lives on the plot(starting at 20140111) | Non-smoking house(starting at 20140111) |LocationDistance nearest habitation (5 m.) | Distance shopping (700 m.) | Distance restaurant (700 m.) | Distance nearest city (Narbonne m.) | Distance Airport (70000 m.) | Distance water (30000 m.) |ConceptPlease not that pets are not allowed in this property, however, we cannot guaranty an allergy free property. |Number of Bedrooms (3)Room:1 Double bed.Room:1 Double bed.Room:2 Single bed.Number of Bathrooms (3)Bathroom:Toilet: Hot and cold water | Shower | Bathroom:Toilet: Hot and cold water | Shower | Bathroom:Toilet: Hot and cold water | Shower | Owner Extra ServicesDeposit(250,00 EUR, Price per stay.) | Bedlinen incl. towel(0,00 EUR, Price per person.) | Consumption costs excl. |
|Sleeps up to 6, 3 bedrooms
|Nearest travel links
|70 km
|Family friendly
|Great for children of all ages
|No pets allowed, No smoking at this property
Features and Facilities
|DVD player
|Shared outdoor pool (unheated)
|Satellite TV
|Washing machine
|3 bedrooms, 3 bathrooms of which 3 Family bathrooms
|Double beds (2), Single beds (2)
|Shared outdoor pool (unheated), BBQ
|Suitable for people with restricted mobility, Not suitable for wheelchair users
This property in Languedoc-Rousillon France is a lovely villa that sleeps 6 people, so it is perfect for a large group of friends. The accommodation offers: a perfect holiday stay.
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Ale of Two Cities
Pecan Point Gastropub & Brewery and the Texarkana Museums System have announced the winner of the "Brand Our Brew" contest -- Ale of Two Cities.
Pecan Point Gastropub & Brewery and the Texarkana Museums System have announced the winner of the "Brand Our Brew" contest -- Ale of Two Cities.
Many people are looking to help flood victims. Anheuser-Busch has found an interesting way to do their part.
Say it ain't so! With a slap to the face of every redneck, country hick, hillbilly and hipster in America. Pabst Blue Ribbon has been sold to Russian company Oasis Beverages, after 170 years in Milwaukee.
Austin Beerworks has been brewing Peacemaker Anytime Ale since they opened up, and since everything is bigger in Texas, and it has never been done, check out the 99-Pack.
Let the celebrating begin! A new survey says that beer is tops for Americans.
The guys are known as the Bottle Boys, and although it's hard imagine someone as an actual musician that plays beer bottles, there's nothing else you can call these guys.
Downtown Texarkana will soon be home to Texarkana's first Micro-brew pub, get ready to welcome the Pecan Point Brewing Company!
The Brewers at Brewmeister have done it again, breaking their own record with "Snake Venom" which has won the title for "2013 Strongest Beer in the World" at 67.5%.
Pigs and hogs might be a nuisance, but not in the way you may think!
Some folks drink a microbrew or two, and the next thing you know they think they are experts on Beer. Sorry, but they aren't. Here are a some beer facts we recently learned...
What started out as a novel idea has turned into a corporate tug of war, as two Columbian brothers are now deep in the trenches of a legal battle with 20th Century Fox for the right to brew beer inspired by the hit television series 'The Simpsons.'
Soda die-hards know if you want a kick of caffeine, Mountain Dew is the beverage of choice. And in some cities in the US, people will soon get to try out a malt-flavored version of the popular soft drink.


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Farnaz on Featured, Negating Stereotypes, Redefining Archetypes, Women, Emerging Power
Negating Stereotypes: Muslim Women Creating Fastest Cultural Shift
Did you know some 800 million population of Muslim women surpass the combined populations of the United States, Russia and Brazil? Stereotyping Muslim women and putting them in one box would be the same as putting all characteristics and cultural nuances of all these three big countries into one box.
Did you know Islam is the only major religious group projected to grow faster than the world's overall population? According to the July issue of Time magazine, from 2010 to 2050, estimated growth rate of the global Muslim population from 2010 to 2050 is 73%. Stereotyping this largest global population cohort would require a very limited and narrow mindset, don't you think? Yet, we hear it in the mainstream news all the time—and even from some liberal satirist like Bill Maher—not to mention politicians who will use anything for political gain. Isn't it time to confront this huge bias, prejudice and past orthodoxies?
I am a Muslim woman. I've never truly studied Islam, nor consider myself a religious person. Sure, we can always pick and choose what studies we like and not choose those that don't fit into our own personal beliefs and values. And there are always two sides to all stereotypes. But I must admit, I am appalled by the consistent generalization and stereotyping of Muslims, as I would be by racism and sexism. Yet, this blog is not about Islam. I won't even attempt to claim myself as a thought leader in this area. But if you'd like to learn more, as I am, I recommend reading No god but God: The Origins, Evolution, and Future of Islam, by Reza Aslan, or at the very least, this McKinsey article by William McCants.
However, I am a thought leader about The New World Marketplace and the cultural macro trends with women, youth and multiculturalism. And I'd like to dedicate this blog to the Women in the Muslim world who are taking the fast track to big cultural changes. Be ready to realize that this largely unseen population of Muslim women will soon become a cultural force to be reckoned with.
In my book, I punched a hole in the western feminist movement believing that it's about shifting the power…trying to convince us that religious and cultural traditions must be overturned for women to be liberated and equal. I disagree. I believe it's about sharing the power. I believe tradition is very different than oppression and feminist success means overturning suppressive energies of any kind.
Muslim women around the globe invest in tradition to create change and make a point. They wear hijabs to have the freedom to talk to leaders and create the cultural change. Sakena Yacoobi is an advocate for women's rights in Afghanistan. She believes and speaks loudly about the fact that Koran requires and strongly supports literacy. And she uses her religion and tradition for this cause. She is a devoted Muslim Woman activist. She believes change has come through partnering with communities, building trust, providing quality services, and waiting for results to become self-evident, rather than speeches, marches or laws. Today, assumptions have changed from "women don't need education" to "education is valuable for all."
According to a recent Mckinsey study, while much work remains to close the equity gap for the 800-million Muslim women worldwide, the rates of education and employment for some have increased dramatically in a short span of time. Changes that took half a century in the United States are being compressed into a decade in today's Muslim world, and they are likely to accelerate.
In the space of two generations, a widespread education movement has elevated the prospects of millions of Muslim women, from Tehran to Tunis. You've often heard my sound bite: for every 2 men graduation from college, 3 women are and with better GPAs. This is not just a US phenomenon. In Algeria, Bahrain, Jordan, Kuwait, Lebanon, Oman, Qatar, Saudi Arabia, and Tunisia, university-enrollment rates for women now exceed those of men. It is not the gender equality that is so massive, it is the rate and pace of this acceleration that is massive and underreported. In Egypt, there were 3 women for every 4 men in universities a decade ago … today, they are equal. In United Emirates, women enroll at three times the rate of men. The list goes on. I often wonder what that means for the new male generation being raised by these educated women? Would we have as many religious wars?
Nearly 40 million Muslim women have joined the labor force. Clearly, there will be more and the next wave of change is under way. And for marketers, we are talking about unprecedented consumer power. According to McKinsey, in the next 15 years, even if women participation in the workforce reaches two thirds of men (or around 60%), it has the potential to spike regional GDP by 20% or more.
I believe Feminism is not just about the struggle against the ruling oppressor, but also about assumptions held by all of us, including women. This means a deep-rooted realization that women don't have to look like a high-powered Western businesswoman to be party leaders and game changers. They can use their own religion and culture to navigate the social and cultural change.
Please share if f you like this blog, and let me hear your thoughts.
Big Generational Shift: Gen Y women possess new world leadership qualities and will break the glass ceiling
In The New World Marketplace, businesses are redefining the new leadership skills and capabilities required for building and sustaining successful and profitable businesses. And Gen Y women have a natural advantage in contributing to this new leadership construct.
All the research I've done over the years suggests that Gen Y women are far more career focused, confident of themselves, have clear views on equality of men and women at workplace as well as at home, and have demonstrated that with passion and commitment with their families, versus prior generations. They are able to grow successfully with the progressive organizations and make a significant impact.
But don't take my word for it. A McKinsey report shows that companies with gender-balanced executive committees have a 56% higher operating profit compared to companies with male-only companies. Another study conducted by Catalyst shows a 26% difference in return on invested capital between companies with 19-44% women board directors as compared with those who had no women on their boards. Hence increasingly businesses are working towards hiring and retaining larger percentage of women in their workforce.
Why are women contributing to higher success? These studies indicate reasons through women's unique characteristics that businesses can benefit from namely, multitasking, paying attention to detail, conflict resolution, ability to deal with fuzziness, flexibility and creativity required for problem solving. These are some of the key capabilities in demand today as businesses are redefining the new leadership capabilities required for building and sustaining successful businesses.
Those of you reading my blogs regularly know that I stand against stereotyping based on gender. So, I must add that men possessing these qualities are and will be just as successful of leaders as women—yet, it's hard to dispute that women in general, and Gen Y women in specific, have a natural inherent advantage with these qualities and are not afraid to express them.
This article suggests that another significant factor that makes Gen Y women stand out is the fact that they have embraced digital technology with ease. They are savvy consumers of technology and are certainly more comfortable with gadgets and devices as compared to Gen X women and on par with Gen Y men. Technology has played an important role in liberating Gen Y women from lack of awareness and exposure to the world at large and making them more confident. IT/ITES industry in India has close to 30-35% Gen Y women as part of the workforce competing for prime career opportunities impacting the global corporations with their technology prowess.
And of course, you've heard me say for years that Gen Y women view success as being able to shape their own path and future. Sure, they like to get married and have kids, but not at the same rate as previous generations, and certainly not at the expense of their career and social/cultural equality with men.
So, here's the big question: Will Gen Y women be first to break the glass ceiling? Research predicts YES….!!!
We keep hearing from feminist leaders that despite all the empowerment initiatives out there for women, at the current rate, we will never close the gender gap in business leadership. And I've been saying all along that is not true….not at the current rate of women in mid-management levels, rapid rate of retirement for baby boomers, and the business cultural shifts we are experiencing. Now, there is research from global talent solutions company, Hudson, supporting my prediction and proving that women in their twenties and early thirties will be the first generation to break the glass ceiling.
According to the results, which analysed 28,000 psychometirc tests across 20 different countries, Gen Y females scored 18% higher than Gen Y males on organization, 10% higher on people skills and 12% higher on social confidence. When compared to Baby Boomer males, the difference in skill areas became more acute: Younger females ranked 16% higher on people skills, 22% higher on social confidence and 21% higher on ambition.
As it relates to traditional leadership skills, this research shows baby boomers were 28% more decisive than Gen Y….and Gen X 13% more strategic than Gen Y. I think this is certainly correlated to age and experience on the job.
The need for persuasive, confident and extravert leaders has been replaced by socially confident and organized bosses, who have the people skills to manage the shifting demographics of tomorrow's workforce.
Simply look around, and you will see that today's workforce is truly multi-generational. This is a global phenomenon. And with the rapid rate of retirement of baby boomers, the work force is being replaced by a generation with huge psychological differences and gender balance. And once again, the leadership attributes found in Gen Y women display attributes of tomorrow's leaders.
It's true that 80% of executive directors on the boards of the FTSE 100 may currently be male, but the findings of this research show that, as business practice continues to evolve and progress, Gen Y women are better placed than ever before to position themselves at the top of businesses over the next decade and possess all of the right skills to help them navigate a technologically data-driven future.
"With their chart-leading altruism and optimism, and their progressive people skills, these women will lead by laying out a vision and welcoming those who want to take part," the report said.
The New World Marketplace leaders and bosses will look very different than the leaders and the bosses we have today. They must understand these disruptive cultural and demographic shifts and support the generational evolution.
Are you ready to shift in 2015?
Study shows women and millennials in leadership roles translates into higher success
Finally….!!! There is a study that proves what I have been saying for years. This study shows that when women and millennials (aka Gen Y) are in charge, big things happen and organizations succeed. This is not a passing trend. Having millennials and women in leadership positions directly correlates with the success of a company.
The Global Leadership Forecast looked at the workforce issues affecting 13,124 leaders from around the world, representing 48 countries and 32 major industries. Of the participating organizations, those in the top 20% financially had almost twice as many women in leadership roles, as well as more high-potential women holding those roles. Does gender diversity pay off? Yes…!!! Absolutely…..!!!
Also, companies with a 30% proportion of young people in higher roles saw "aggressive growth," according to the study. When it's more like 20%, they saw "little to low growth" rates. Granted, they leave organizations faster (within a year) and less engaged than other groups, but those are opportunities for companies to overcome. But how can we ignore the success results of having women in leadership roles?
Even beyond the business success, there is all the life ending crises around the globe. Heart breaking. I couldn't even get myself to write a business blog lately. Who would want to read about cultural change and macro trends when faced with such never ending wars of domination and control? I even read Gaza-tweets saying women should stop having children until men learn not to kill them. Then I thought, it's the leadership, stupid….always has been and always will be. What this study doesn't cover is what would happen if we had more women and millennials in political leaderships. Would things be different? I'd say…Yes….!!!
Sure, as long as there is life, there is death. Can I say as long as there is love, there is war. Maybe. But can we move the needle by stepping into the New World order and starting to change our existing homogeneous leadership in businesses and politics. If not for a better world to live in, for higher profit for all. Let's start sharing these studies.
Work/Life Balance is no longer a gender issue, it's cultural and generational
Work/Life balance has been in the forefront of business productivity plans for over a decade as more women entered the work force in management positions and contributed to better company performance. What started out as a gender issue, later became a generational issue, and now it's a cultural issue. With 70% of US moms working in 2014, the cultural conversation has shifted from whether women should work outside of home to how do we understand the needs and values of the working parents, and tailor policies accordingly, to attract and retain the best talent regardless of gender and/or generation. Talent is a company's most powerful sustainable competitive advantage. These valuable insights are extremely important in branding strategies of your value propositions to the new world marketplace customers as well.
WMRI (Working Mother Research Institute) recently conducted a fascinating study on 3 generations of men and women that make up majority of the work force now: Baby Boomers (1946-1964), Gen X (1965-1980) and Gen Y, aka millennials (1981-2000). Interesting similarities and differences between generations of parents as it relates to the best ways to manage work and personal lives….clearly common grounds for all working parents as well as areas where generational and gender attitudes diverge…..
Do you remember Gen X being referred to as slackers years ago? They earned this reputation mainly because they were the first generation that demanded work/life balance. For work-centric boomers, they were slackers. Can you imagine what Boomer bosses are going through now with Millennials? I've spoken to many business leaders who stereotype and mischaracterize Millennial's love and expectation of work/life balance as laziness and lack of ambition, versus a cultural shift and thoughtful re-prioritization of personal and family values.
This WMRI extensive study clearly outlines that Gen Y moms feel far more optimistic and happy than previous generations—mostly because of the support they are receiving from their spouses (as equal active partners) but also from their parents and managers. Gen-Xers tend to be stressed often viewing work as a financial necessity…but they are ambitious, highly educated and tech savvy, and overwhelmingly part of the dual-earner couples. And baby boomers feeling stretched as new demands of caring for parents, retirement and aging are upon them….hard to imagine when they find the time to look and feel younger than their age. The only group that outshine Millennials in their optimism is the female breadwinners—in every category. Interesting, huh?
Couple of surprises about Millennials, even for me…although they are cheery about family finances (64%), pleased with their relationship with their spouses and partners (71%) and were even happier about contributions fathers make in caring for their children (73%)…and although this generation is more career-minded than the previous two….they are more conflicted about child care and career. Most agree that one parent should be home to care for the kids (60%) and home when a child gets there (83%). And this is the generation that feels free to choose between work and staying home and they figure out how. Don't be surprised if Millennial parents choose to stay at home for some years during their career. Providing flexibility may or may not be enough for them. More than half of all men and women in Gen Y think flex causes work to interfere with family time. So separation of work and family is more important to them. And encouraging unplugging even more so, specially at nights, weekends, vacations, etc.
Depending on your perception, this may emphasize some stereotypes about laziness and sense of entitlements for this generation, but like it or not, this is the future work force. Millennial's need to unplug is not to avoid hard work, but to help them fuel for the job—whereas boomers and Gen X'ers liked the always-on, always-connected work culture that gave them flexibility of working remotely. Let's not mischaracterize these cultural shifts in needs and values. The always-on fatigue is somewhat evident in previous generations.
What everyone wants most in the workplace, no matter the gender or generation, are job security and stability, competitive earnings and flexible schedule and culture. But communicating each for different generations may be very different. The key is ensuring that the flex options match the needs of each life stage.
Here's the common ground regardless of gender or generation…top 3:
- Couples should have equal input of how income is used no matter who earns what
- Children should be cared for equally by both parents
- Work and personal time should be kept separate
Now ask yourself if your current business practices and policies support these values? How about your branding and marketing strategies? I'm starting to see a few fathers in commercials but are we representing working parents both equally caring for children and deciding on spending? Think about it…this is a huge opportunity.
I have written about many studies that have shown that Millennial dads are more enthusiastic and engaged with their kids than previous generations. The latest Pew research released this month revealed that more dads are staying home with the kids. Although this study did not break down by generation, it showed an overall rise of stay at home dads in recent years. High unemployment rates around the great recession contributed to this increase, but the biggest contributor to long-terms growth is number of fathers who stay at home primarily to care for their family. Even more interesting and noteworthy is that working fathers with children under 18 are just as likely as working mothers to say that it is difficult to balance work and family. Roughly equal shares of working fathers (48%) and mothers (52%) prefer to be at home raising their children, but they need to work because they need the income.
These are the trends that will likely have dramatic impact on workplace policies in the not-so-distant future for both mothers and fathers. And they should have dramatic impact on your marketing and branding strategies today and onward. Do you want to have a powerful competitive differentiation and advantage? Start embracing these cultural shifts in needs and values.
Are you ready for the shift?
Keep staying informed, and please share your thoughts and comments below….
Do you know the underlying needs and values to address women effectively? 10 questions to ask your strategy team
Since the Wave 5 of the Ipsos MediaCT Audience Measurement Group came out earlier this month about Women, Power & Money, I read myriad of articles on the web. There was one in particular by MediaPost which intrigued me, examining American women's lives, lifestyles and marketplace choices across three generations—Gen Y, Gen X and Baby Boomers. I thought the findings identified cultural shifts in women's priorities and how women are shaping the leadership and financial course of The New World Marketplace. Of course, as an X-CMO, I thought they were all missing what companies and brands should do differently with their strategic positioning and branding messages with these new findings. But that is why you are here, reading my blog.
There is no doubt that, despite gender lags in pay and salary negotiations, American women are feeling increasingly empowered, independent, knowledgeable and successful. According to Allianz women money and power research, women made up half of all stock-market investors and controlled 48% of estates worth more than $5 million in 2006-2007. By 2011 women controlled over 50% of the United States' wealth, and 60% of women with business degrees out-earn their husbands and describe themselves as primary breadwinner. And according to the latest U.S. Census, regardless of educational attainments, women out-earn their male partners in 22% of households….while this is not a big number worth bragging, it is a far cry from Cinderella archetype.
However, there are radically differing perceptions of financial responsibilities between women and men, says the report. Women perceive controlling day-to-day spending, with ¾ or more feeling responsible for household purchases, while big-ticket purchases are considered joint responsibility. Men perceive differently, seeing day-to-day decisions jointly, and big-ticket purchases as largely theirs. Regardless of this differing perception, it makes sense, in any healthy relationship, to discuss and agree on big-ticket and joint-household purchases…while day-to-day spending may not warrant negotiations. The same is true in any Corporate structure of financial responsibilities and sign-offs, isn't it? This speaks greatly to who should be targeted for what product/service purchases, singularly or jointly, varying by age/generation, culture, income and lifestyle.
Let's face it. Since the recession, messages of price value and affordability resonate across genders, cultures and generations. But throughout the Ipsos study, women show greater tendencies toward price and value (despite income), more inclination to spend on "experiences", and more openness to new brands….which make them less brand loyal (only 29% express brand loyalty). Men are more likely to spend on products, less price focused (except for financial services) and show preference for familiar brands. For women, the security and freedom money brings is 15-20 times more important than the status and respect it affords.
This report also highlights key generational differences:
- Boomer women perceive more differences between men and women. However, in my opinion, this is the generation that taught Gen Y about gender equality and "girls can do anything boys can do." The study shows that they are more swayed by messages related to "values" and corporate social responsibility, but I believe they are also leading the way with embracing the major cultural shifts for the younger generation…for their sons and daughters.
- Gen X women are solidly in the lifestage of family formation and its associated trade-offs. They seem more financially constrained and price-conscious—so price/value messages resonate best with this generation of women, and considered necessity.
- Gen Y women, aka millenials, feel empowered and equal to men, and are more likely to describe themselves as smart (70% vs. 54% men). But they also feel more stressed and exhausted in an uphill climb in achieving equal results with men. Gen Y is also a global generation of women with perspectives and marketplace preferences that transcend gender and cultural borders, and are inspired by shared experiences of technology, innovation, social media, and new creative brands.
I believe it is the Gen Y women that will finally close the gender inequality in corridors of power in the future. This new generation of women not only feel more ambitious, independent, smart and educated, but they are also less likely than men to be living with their parents—32% versus 40% of men–continuing a long-term gender gap in the share of young adults living at home, according to Pew research.
In my book, I cited the Levi Strauss Millennial study that showed values such as independence (96%) and being able to shape their own future (87%) trump everything… including becoming a mom (68%) and marriage (only 50%). This generation of women who grew up with executive mothers see the hard-working, hard-charging work life as "extreme" and costs too great. This is the most educated cohort of all times with a zest for entrepreneurship, if for nothing else, so they can shape their own future. So clearly they have the greatest influence on cultural evolution for women. (Also read, Evolving Archetypes & Rise of Women)
If you think about it, these underlying "values" and "needs" have major implications in building emotional connections through your branding and communication strategies and messages. More importantly, they help define need-based targeting for brand products and services. For example, price/value is increasingly becoming a greater and greater "need" for women in providing quality life for families, and brands have greater and greater "need" to differentiate amidst the clutter with lower brand loyalties among women consumers. Generational life phase clearly bring forth different set of needs, but the aspirational values for women cross over generationally and demographically.
Targeting women, in general, is an economic imperative and strategic necessity for profitable growth. Targeting women effectively can also serve as a key strategic differentiation for companies. Women not only control majority of buying decisions, but they also demand change and expect it to be meaningful.
If I was consulting for your company, I'd start with asking your strategy and marketing teams these 10 key questions. Here they are…go ahead and ask your team….this is good starting point for your strategic discussions around your Value Propositions and branding/communication strategies:
- Have we re-evaluated our core target to primarily include women ?
- Have we defined which women, which needs and at what relative price?
- Does our Value Proposition(s) identify and align with evolving needs and values?
- How do we differentiate from competition? Is this clearly being communicated?
- Do we know which one of our products/services is "her decision" alone, and which ones are joint with her partner? Are we communicating accordingly?
- Are we enhancing "her experience"? If so, how are we communicating this?
- Does she consider us "affordable" relative to competition? (Note: affordable is not the same as cheap)
- Are we avoiding gender biases and stereotypes in our communication strategies?
- Have we identified the sweet spot of commonalities cross-generationally?
- Are we recognizing and acknowledging The New World Modern Woman?
Can you and your team answer these questions effectively? Are you ready to shift?
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Do women hold themselves back from corridors of power? My take on "Lean In"
It took several weeks of unusual summer rain in Atlanta and couple of days without phone, internet and cable, before I finished reading some of the books laying on my night stand for months. One of them, Lean In, by Sheryl Sandberg.
I was among many women who bought this book due to all the media hype, but didn't read it immediately. Mainly because there were so many articles, blogs, reviews, videos and TV interviews, that I felt I had already read the book. But not true. The promotional sound bites and all the controversy don't do this book justice. I must admit, reading her 'acknowledgements' section, which was as big as any chapter in the book, made me think that if I only had that much help with my book (or any help), I would end up with the same media coverage. Envy aside, kudos to her and her team….I absolutely love her message and her courage to start this important dialogue, and believe her book is a must read for all women and men. And here's why…..
Sheryl Sandberg is the first woman who finally spoke up about internal barriers as much as external barriers that hold women back from reaching leadership positions. It's true. Many women (not all) hold themselves back by lacking self-confidence through gender stereotype messages they hear and tend to believe throughout life…. by not raising their hands or sitting at the table…. and by pulling back when they should be leaning in (leaving before leaving). Her TEDtalk speech with this main message reached over 550 million views. In the book she goes further into complex challenges women face and adjustments/differences we can make ourselves: increasing self-confidence and closing the ambition gap, getting our partners to do more at home (Make Your Partner a Real Partner)… not holding ourselves to unattainable standards (The Myth of Doing It All)…. and of course my favorite–avoiding gender stereotypes (OK, she doesn't have a chapter on this, but she references it allot and I'm personally all about this.)
All that sounds great, right? I nodded my head in agreement most of the entire time and even laughed out loud quite a few times (it's a witty, funny book). So why all the controversy, and why are so many women so pissed off? Sheryl may think it's because success and likability are negatively correlated for women, by both women and men. She has a chapter on this with great research no one can negate. But I think there is more….I believe there are two main reasons at the core of the controversy and mixed responses by women about this important message:
First, many women don't face these internal barriers, depending on cultural and family backgrounds, but face complex external barriers that hold them back. It's important to read this book without reaching any conclusions, because her argument does not negate external barriers, but pushes everyone to get rid of internal barriers critical to gaining power and success. She poses this as the ultimate chicken-and-egg situation. The chicken: women will tear down the external institutional barriers once we achieve leadership roles and make sure we level the playing field (one can shoot holes all over this with research too.) The egg: we need to eliminate the external institutional barrier to get women into those roles in the first place. Both valid, she encourages women that instead of debating over which comes first, to focus on the chicken and our own internal barriers. It's a brave move and rarely discussed. Problem is, without reading the book, some think she is attacking the victim. That is not true at all, but perhaps her marketing team have not done a good job balancing her promotional/media messages to include both, as she has done in her book, without alienating those women who truly know how to "lean in" but face institutional barriers. Even those women can really benefit from her message of unconscious gender bias and stereotypes that women face ourselves, and against one another.
Second, and more importantly, I think people in general (men and women) have a harder time relating to those who have not been through similar experiences and hardships. Overcoming severe adversities is a common thread among greatest leaders….one that entices people to listen, relate, learn and follow. And Sheryl has not been through much other than difficult child labor and long work hours. No one should ever hold that against her and the important message she is sending out to The New World Marketplace. But this lack of common wo/man relatability goes far beyond a correlation analysis of success and likability. We all love–regardless of gender, age and culture–hero/eins who rise from ashes like the Phoenix, overcoming adversities and helping others.
With all that said, I give "Lean In" a big thumbs up. But I'd only re-write Time's headline, "don't hate her because she is successful," and say, read this book regardless of how relatively easy success came to the writer, because the message is excellent, prevalent and important for all women and men cross-generationally. Bravo Sheryl.
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New World Trend & Cultural Shift: Is Dad the New Mom?
We are used to generations of women doing the lioness' share of child care and housework, even if they have jobs outside the home. Now we are seeing rise of co-parenting and cultural shifts phasing out "husband and wife" and "father and mother" and replacing them with functional roles of "spouse and parent." Work-family balance is no longer a women's issue—it is now truly a "family" issue as the word intended.
According to the most recent Census report, the number of stay-at-home fathers in the United States has more than doubled in the past 10 years to 176,000. And according to a report released by the Family and Work Institute last year, men are also experiencing work-family conflict, with 60% saying it was an issue in 2008 (up from 35% in 1977.) That figure remained relatively flat for women (47% in 2008, 41% in 1977.) Today's Gen Y dads, aka millennials, spend 4+ hours per day with kids under 13, versus only 2 hours in 1977.
A similar WSJ article reported from Census that 32% of fathers with working wives routinely care for their children under age 15, up from 26% in 2002. Pew studies report that dads have tripled the amount of time they spend with their children since 1965. Myriad of research showing increased share of household chores by men…not surprising given the increased presence of women in the workplace, right? But the world outside of homes and inside marketing/branding meeting rooms haven't caught up yet.
New World fathers are no longer seen as just financial providers or occasional babysitters. They are actively engaged in their children's daily lives and routine care and view fatherhood as a big part of their personal identities and a pride attribute of who they are as individuals. Factors vary from job market and increasing cost of child care, to rise of women at work, blurring gender roles in the youth culture, and to a degree, today's men raised amid the women's movement and perhaps absent fathers… But, no one can argue that the new world of more involved dads as full time partners in parenting has arrived and it's here to stay.
What's even more interesting is what Pew Research calls "breadwinner moms." A record 40% of all households with children under the age of 18 include mothers who are either the sole or primary source of income for the family. The share was just 11% in 1960. One of my continuous sound bites about The New World Marketplace is that 1/3 of Gen Y were into unwed mothers.
These "breadwinner moms" are made up of two very different groups:
1) 5.1 million (37%) are married mothers who have a higher income than their husbands, and are slightly older, disproportionally white and college educated…grown from 4% in 1960 to 15% in 2011.
2) 8.6 million (63%) are single mothers, who are younger, more likely to be black or Hispanic, less likely to have a college degree, grown from 7% to 25% during the same period. And they are more likely to be never married than divorced/separated.
No surprises here, education has always had direct correlation to income, and unfortunately to date, correlation to race/ethnicity (but this is changing.) Interestingly, both groups of breadwinner moms have grown in size in the past as seen by increasing work population of women. What may be surprising to most is that the total family income is higher when the mother, not the father, is the primary breadwinner. And married mothers are increasingly better educated than their husbands. This is a trend most likely to escalate as we see for every 2 men graduating from college, 3 women are and with better GPAs.
What do all these cultural shifts mean to you and your businesses?
It's simple. Think about it. Should diaper bags and child care materials all have pink bows and flowers on them? Diaper Dude now sells dozens of styles of bags designed to appeal to men…grey, black, camouflage prints, even bags with baseball team logos. Are you in the restaurant business? Have you thought about changing tables in your men's restrooms? Are you in technology business? Think of the AT&T ad showing a dad changing diapers while talking sports on his smartphone with his friend.
The new generations of parents use technology to feel connected and involved with their children. It's no longer just about reading the popular books on parenting, but also weekly customized e-mails from BabyCenter, apps like Contraction Timer, iPads at daycares logging activity throughout the day, watching your kids on your smartphones from your office. Even doggie day cares allow that. But why aren't we seeing enough of these new world life scenarios in advertising campaigns for technology brands, specially using dads? Working moms, hands-on dads and more involved young fathers are the new normal. Think about that next time you are developing an ad campaign for a household product.
This type of cultural trend has significant impact on traditional paradigms and how marketers should view targeting families for products and services.
Yes… Women control 85% of consumer buying decisions. Moms will remain a key target market for many business categories. But what do you think appeals to women and moms? Certainly not the old gender stereotypes.
Here are 3 simple tips to get you started:
1. Don't speak to mom at the exclusion of dad, unless you are targeting single mothers only …he is a trusted parenting partner.
2. Avoid all gender stereotypes in your branding messages and strategies. Market to shared values and needs, not gender. Market to the inside of your customers, not outside.
2. Don't project your own traditional cultural paradigm in your branding strategies. You are not your customers. And it is The New World Marketplace, afterall.
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Cultural Change Expert explains why women don't need to man up to be successful leaders
FOR IMMEDIATE RELEASE (by Tate Publishing)
ATLANTA, GA. – It's The New World Marketplace afterall, and women, youth and multiculturalism are shaping our future. A rapid cultural shift has occurred over the last decade, but author and thought leader Farnaz Wallace believes it's not being addressed by businesses and leaders.
"With all the great empowerment initiatives for women today, we are still looking at massive gender inequality in corridors of power," she said. "Many great women thought leaders point to re-evaluating business policies to eliminate obstacles that force talented women to choose between family and career, and others point to women's ambition calling us all to man up–but I believe there are 4 missing links from this important dialogue."
Wallace breaks these down into four essentials for women to succeed in The New World Marketplace Leadership: Inner-authenticity, Being the woman leader other women want to work for, Re-defining Power, and Avoiding all gender stereotypes.
"I've always believed once you gain trust and respect, love always follows," she said. "But how can women gain trust and respect if they're trying to be someone they are not?" Wallace believes it's far more important to find the richest, fullest expression of one's authentic self, and spend majority of time in strength and passion and generate results, versus trying to fit into a perceived cultural norm.
"Research shows that women have a tougher time working for women," she said. "To be a successful leader, people of all ages, cultures, race and gender must want to follow and work for you, specially other women. Men and women largely agree on life goals. It is the position of power and domination that differentiates us, not just between men and women, but also among women ourselves."
"That's even more of an important toptic than gender inequality because the old business culture of command and control doesn't work for men or women," she said. Wallace believes it is time to redefine power as less need to limit or control others, and define power as affiliation, linking and partnership–a blend of hard and soft powers, she calls it "smart" powers. "Women don't need to man up to be successful, they need to possess smart powers," she said.
"Women are different than other women, just as men are different than other men, why the continuous focus on gender stereotyping?" she asks. "I believe if we want to achieve gender equality, we must first stop gender stereotyping for it serves no purpose other than protecting traditional orthodoxies that have held women back for generations."
Wallace is a thought leader, speaker, and strategic consultant focused on helping companies capitalize on cultural macro trends in today's fast-changing marketplace. She is the published author of the book, The New World Marketplace, and presently resides in Atlanta.
4 Tips on How to Become A New World Woman Leader (Video/Blog)
With all the great empowerment initiatives for women today, we are still looking at massive gender inequality in corridors of power. Many great women thought leaders point to re-evaluating business policies as it relates to the flexible work schedule and eliminating obstacles that force brilliant women to choose between family and career. Others point to women's ambition calling us all to man up. While I think there is truth in both, I think there are 4 missing links from this important dialogue.
In my keynote speech at Possible Woman conference two weeks ago, I addressed what it takes to be a successful woman leader in The New World Marketplace. Here's a 5-min video highlight of this keynote address followed by my written summary of the 4 tips I shared:
1. Be authentic, focus on your own unique differentiation, gain trust and respect
I wasn't just a woman working hard to advance my career in Corporate America, I was an Iranian-American woman….so you can just imagine the brutal stereotypes I had to face and overcome. I wasn't just an Iranian-American woman, but I had multi-colored hair and tattoos. A far cry from a traditional image of a successful businessman. But at the end of the day, results speak for themselves. Under my CMO leadership, we drove 5 years of consecutive same-store-sales growth. So, I built trust and respect instead of focusing on changing myself to fit into a cultural norm…and I've always believed once you gain trust and respect, love follows. Trust and respect are two most important shared values in relationships in The New World Marketplace. But how can you possibly gain trust and respect of your collegues, employees, bosses, even your customers, when you're trying to be someone you're not?
It's far more important to always find the richest, fullest expression of your authentic self, and spend majority of your time in your strengths and passion, versus trying to fit into an exclusive image of the professional businessman, which is no longer the success archetype in The New World Marketplace. Because being good at what you do has nothing to do with how others see you…but it has everything to do with how you see and feel about yourself.
I not only believe the inner authenticity translates in to your own power and success, but I believe authenticity in branding strategies also translates in to your company success. Strategy is not about being the best, it's about being different and unique. Your own branding strategy has to be the same….about your own uniqueness and differentiation. Be authentic, focus on your own unique differentiation, gain trust and respect and let the results speak for themselves.
2. Be the woman leader other women want to work for
Beyond my non-traditional image and my business and financial performance, it was my style of leadership that differentiated me. I was determined to become the woman leader other women wanted to work for. As an emerging leader, I was always promoted every 2-3 years by working hard and driving results. And I've had just as many female bosses as I've had male bosses….yet the women bosses weren't the ones promoting me. I always questioned whether it was a scarcity mentality that there is just not enough abundance to go around for all of us…or a flaw in leadership training for women. I decided then that I wanted to be a woman leader other women wanted to work for and that I'd provide an environment for women to thrive and succeed.
As I was doing research for my book, I came across this data from Time Magazine: More than 2/3 of women still think men resent powerful women…yet 45% of women say female bosses are harder to work for, versus only 29% of men. This is a major issue not often addressed.
Believe it or not, men and women of all races and ages largely agree on life goals. It is the position of power and domination that differentiates us, not just between men and women, but also among women ourselves. That's even more of an important topic than gender inequality, because one of my biggest fear is reaching gender equality but maintaining the same business culture of domination, command and control. Because that model is not working, and simply switching gender without redefining power and success will not address the core issue.
3. Redefine Power as a blend of hard and soft powers – SMART powers
I believe it's time to redefine power as less need to limit or control others and define power as affiliation, linking and partnership. In fact, the need to control and dominate, in reality, is a feeling of powerlessness. That means leaving behind the hard, conquest and domination-oriented values. I don't believe you need to man up to be a successful woman leader.
Feminine values or soft powers are loaded with polarizing reactions, but they are meant to refer to values associated with creation, life-generating, nurturing powers, caring, relating…human and relationship values that have become a business imperative and taught in almost all leadership materials…versus taking, conquest and domination.
It's time to use feminine and masculine powers as qualities and values in all women and men, instead of gender stereotyping. Let's face it…there are many women who lead with masculine hard powers, and there are many men who lead with relating, nurturing, caring & soft powers. It has nothing to do with gender.
I believe we all need both…. and it's a matter of knowing when to use which… Blend of hard powers and soft powers….let's just call it "SMART POWERS." It's time for women leaders to re-evaluate how they view power to succeed and use SMART powers.
4. Stop gender stereotyping, avoid focus on gender differences
Men are from Mars. Women are from Venus. Ask yourself why do we persist to focus on gender differences. Men are logical. Women are emotional. If that's true, and marketing is designed to get an "emotional" response and attachment from customers, why do we bother market to men at all? Men are different from other men. Women are different than other women. Why the continuous focus on how men and women are different? Haven't we confused our next generations of leaders enough?
I, for one, have defied all gender stereotypes, but that doesn't mean I forgot how to be a caring, nurturing woman as a leader. I used my masculine powers to gain competitive market share and drive financial results, but I led my team with feminine powers of caring, relating and partnership.
The focus on gender inequality must be different than our continuous focus on gender differences. In fact, I believe if we want to achieve gender equality, we must first stop gender stereotyping for it serves no purpose other than protecting traditional orthodoxies that have held women back for generations.
In The New World Marketplace, it's neither the man's world nor a woman's nation. It's a dynamic, cooperative shared reality that is under constant evaluation. And the template of success and happiness is very unique and personal to each individual, and is gender neutral and color blind. If you lead with authenticity, purpose and passion, power and success will follow.
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Farnaz Wallace Keynote Address at Diversity Woman Leadership Conference
Farnaz Wallace delivers a thought-provoking, motivational keynote about The New World Marketplace at 2012 Diversity Woman Business Leadership Conference in Washington DC on December 17, 2012. Here's a short 3-minute clip of this speech:


text:
Mo. teen shot by cop suspect in recent robbery
Ferguson Police Chief Thomas Jackson released police reports stemming from the case during a news conference where he also identified the officer involved.
Join the discussion
PoliceOne top 5
- 1 Dunkin' Donuts employee writes 'Black Lives Matter' on RI cop's coffee
- 2 Viral video: Armed Instagram user stalks Calif. cop
- 3 Racial profiling law is 'terrible' Calif. legislation, police officials say
- 4 Video: Drunk Conn. college student throws fit over mac and cheese, gets arrested
- 5 Another Texas department adds 'In God We Trust' to patrol vehicles


text:
Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping
ABSTRACT Yeast one-hybrid (Y1H) assays provide a gene-centered method for the identification of interactions between gene promoters and regulatory transcription factors (TFs). To date, Y1H assays have involved library screens that are relatively expensive and laborious. We present two Y1H strategies that allow immediate prey identification: matrix assays that use an array of 755 individual Caenorhabditis elegans TFs, and smart-pool assays that use TF multiplexing. Both strategies simplify the Y1H pipeline and reduce the cost of protein-DNA interaction identification. We used a Steiner triple system (STS) to create smart pools of 4-25 TFs. Notably, we uniplexed a small number of highly connected TFs to allow efficient assay deconvolution. Both strategies outperform library screens in terms of coverage, confidence and throughput. These versatile strategies can be adapted both to TFs in other systems and, likely, to other biomolecules and assays as well.
Full-textDOI: · Available from: Vanessa Vermeirssen, Nov 14, 2014
- SourceAvailable from: onlinelibrary.wiley.com
- "The concept of GT can date back to World War II, which was first proposed by Dorfman [Dorfman, 1943], for the problem of determining which blood samples contain the syphilis antigen (defective also called positive samples) for numerous soldiers. For now, many biological applications benefit from the GT such as blood testing [Ding-Zhu and Hwang, 2000; Dorfman, 1943; Sobel and Groll, 1959], HIV testing [Hughes- Oliver, 2006; Kim et al., 2007; Westreich et al., 2008], clone library screening [Balding and Torney, 1997; Bruno et al., 1995; Knill et al., 1996], protein–protein interaction mapping [Jin et al., 2007; Jin et al., 2006; Vermeirssen et al., 2007; Xin et al., 2009], drug screening [Jones and Zhigljavsky , 2001; Kainkaryam and Woolf, 2008; Kainkaryam and Woolf, 2009; Wilson-Lingardo et al., 1996] and population genotyping [Erlich et al., 2009a; Erlich et al., 2009b; Patterson and Gabriel, 2009; Prabhu and Pe'Er, 2009]. Combine with GT, fewer DNA libraries are required to detect rare variants. "
Article: Identifying Rare Variants With Optimal Depth of Coverage and Cost-Effective Overlapping Pool Sequencing[Show abstract] [Hide abstract]
ABSTRACT: Genome-wide association studies have identified hundreds of genetic variants associated with complex diseases although most variants identified so far explain only a small proportion of heritability, suggesting that rare variants are responsible for missing heritability. Identification of rare variants through large-scale resequencing becomes increasing important but still prohibitively expensive despite the rapid decline in the sequencing costs. Nevertheless, group testing based overlapping pool sequencing in which pooled rather than individual samples are sequenced will greatly reduces the efforts of sample preparation as well as the costs to screen for rare variants. Here, we proposed an overlapping pool sequencing to screen rare variants with optimal sequencing depth and a corresponding cost model. We formulated a model to compute the optimal depth for sufficient observations of variants in pooled sequencing. Utilizing shifted transversal design algorithm, appropriate parameters for overlapping pool sequencing could be selected to minimize cost and guarantee accuracy. Due to the mixing constraint and high depth for pooled sequencing, results showed that it was more cost-effective to divide a large population into smaller blocks which were tested using optimized strategies independently. Finally, we conducted an experiment to screen variant carriers with frequency equaled 1%. With simulated pools and publicly available human exome sequencing data, the experiment achieved 99.93% accuracy. Utilizing overlapping pool sequencing, the cost for screening variant carriers with frequency equaled 1% in 200 diploid individuals dropped to at least 66% at which target sequencing region was set to 30 Mb.Genetic Epidemiology 12/2013; 37(8). DOI:10.1002/gepi.21769 · 2.60 Impact Factor
- "Mating experiments were performed as described previously (Walhout et al, 2000; Vermeirssen et al, 2007). Briefly, the two 384-well AD-TF plates were transformed into the Ya1867 strain (a kind gift from John Reece-Hoyes and Marian Walhout, University of Massachusetts Medical School, USA). "
Article: A yeast one-hybrid and microfluidics-based pipeline to map mammalian gene regulatory networks[Show abstract] [Hide abstract]
ABSTRACT: The comprehensive mapping of gene promoters and enhancers has significantly improved our understanding of how the mammalian regulatory genome is organized. An important challenge is to elucidate how these regulatory elements contribute to gene expression by identifying their trans-regulatory inputs. Here, we present the generation of a mouse-specific transcription factor (TF) open-reading frame clone library and its implementation in yeast one-hybrid assays to enable large-scale protein-DNA interaction detection with mouse regulatory elements. Once specific interactions are identified, we then use a microfluidics-based method to validate and precisely map them within the respective DNA sequences. Using well-described regulatory elements as well as orphan enhancers, we show that this cross-platform pipeline characterizes known and uncovers many novel TF-DNA interactions. In addition, we provide evidence that several of these novel interactions are relevant in vivo and aid in elucidating the regulatory architecture of enhancers.Molecular Systems Biology 08/2013; 9:682. DOI:10.1038/msb.2013.38 · 10.87 Impact Factor
- "We therefore decided to construct a Y1H library consisting exclusively of predicted TF DNAbinding sites from B. cinerea fused to the strong yeast GAL4 TF AD. This is the first time, to our knowledge, that such a strategy has been applied to a filamentous fungus, but similar approaches have been described for the study of TFs in other organisms (Caenorhabditis elegans: Vermeirssen et al., 2007; Arabidopsis thaliana: Mitsuda et al., 2010). We report here the construction of a B. cinerea TF Y1H library, its screening with a botrydial biosynthesis gene promoter and the identification and characterisation of a new TF involved in regulating secondary metabolism gene clusters, carbohydrate metabolism, transport, virulence and detoxification mechanisms. "
Article: Screening of a Botrytis cinerea one-hybrid library reveals a Cys(2)His(2) transcription factor involved in the regulation of secondary metabolism gene clusters[Show abstract] [Hide abstract]
ABSTRACT: Botrytis cinerea, the grey mould fungus, secretes non-host-specific phytotoxins that kill the cells of many plant species. Phytotoxic assays performed about ten years ago, have highlighted the role in the infection mechanism of one of these secondary metabolites, the sesquiterpene botrydial. We recently showed that BcBOT1 to BcBOT5 genes, which are required for botrydial biosynthesis, are organised into a physical cluster. However, this cluster includes no gene encoding a transcription factor (TF) that might specifically coregulate the expression of BcBOT genes. To identify which TF(s) are implicated in the regulation of this cluster and thereby to decipher DNA-protein interactions in the phytopathogenic fungus B. cinerea, we developed a strategy based on the yeast one-hybrid (Y1H) method. In this study, a Y1H library was generated with the TFs predicted from complete genome sequencing. The screening of this library revealed an interaction between a promoter of the botrydial biosynthesis gene cluster and a new Cys(2)His(2) zinc finger TF, that we called BcYOH1. Inactivation of the BcYOH1 gene and expression analyses demonstrated the involvement of this TF in regulating expression of the botrydial biosynthesis gene cluster. Furthermore, whole-transcriptome analysis suggested that BcYOH1 might act as a global transcriptional regulator of phytotoxin and other secondary metabolism gene clusters, and of genes involved in carbohydrate metabolism, transport, virulence and detoxification mechanisms.Fungal Genetics and Biology 02/2013; 52. DOI:10.1016/j.fgb.2013.01.006 · 2.59 Impact Factor


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Ecoflow Heating&Cooling Systems
Polish producer of heating and cooling equipment
Polish producer of heating and cooling equipment
Static neutralizer, charge deionizer, discharging electrode, neutralizing static electricity, matchbox, HF technic, industrial automation
Plastic boards, PE boards, PP boards, structural panels, round modules, platform gratings, tanks, pipes, fittings, valves
Jerrycan 5l HDPE
Engineering company for good batchers
RAS Air-cooled Water Chillers - Rental, Service, Used Chillers.
MB Conveyors produces since 1985 high quality belt conveyors, separation devices, elevators, storage lines and automation systems for the plastics and PET industry. We provide custom-made handling solutions for your business!
Ritemp patented cooling technology sets the benchmark for unprecedented savings in plastic injection molding applications, extending to blow mold applications; in early stage development.
Water Chillers, Temperature Controllers, Free-Coolers, Mould Dryers, Special Applications
Frigel is a market leader around the world in plastic process cooling systems.


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Microtel Inn & Suites By Wyndham Ames
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... 100 miles). Ben and his family, warmshower hosts, welcomed us in their home. We loved meeting them and spending the evening with them.
From Des Moines, we went to Brayton, which is about 140km (85 miles). It was quite a long day, probably because we were still tired from the previous day and because of the huge hills of Iowa that nobody told us about :p We were welcomes by Dan, Penny and Hannah, who gave us plenty of food and a comfortable place to sleep. The next ...
That's About It
... Castle (especially if you're a Monty Python or Outlander fan) Culloden Battlefield Places we didn't see enough of or skipped but would love to visit: The Great Glen road to Inverness (we drove part but not all) Lighthouse on the northeast coast above Aberdeen The Cairngorms national park, would stay in it. The Loch Lomond and The Trossachs National Park on the east side including the smaller lochs, we'd spend at least 5 days here in a ...
Day 2: Iowa with Friends
We departed Wichita this morning continuing north on Interstate 35 towards Kansas City and eventually arrived to Iowa for my first ever visit to the Hawkeye State as it's known. But I soon found that it is really all about the corn here: miles and miles of perfectly laid out cornfields in a beautiful deep green color - very pleasing to see driving down the road at 75 MPH.
We arrived in West Des Moines about 5pm to ...
Glow Goo
Can't seem to get going going. Waiting for a fax then we found another flat tire on the tractor. Have to wait forever in freezing tire shop for them to put a new one on. Poor Jp is exhausted. We don't get on the road until about noon. We drive for ...
Sweet corn of mine - Iowa
Hi there We bid farewell to N'Orlens en route to see our friends Ryan & Kiley who live in Iowa. Pete went to High School with Ryan. Many moons ago, Ryan went travelling, met Kiley in Vegas, they fell in love, got married 5 years ago and moved back to Iowa a year or so ago. Timing of it all couldn't of been better for a weekend visit, as Ryan's parents were also visiting from Perth and it also fell on the 4th of July, Independence Day. Whilst we've been travelling through the U.S. ...


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Rolling Hills Golf Club (CLOSED)
- 2200 Candies Ln NW, Cleveland, TN 37312
- (423) 472-7129
|18 total holes at facility
|Green fees - $21 to $31 (estimate)
|Built in 1964
|Driving Range: Yes
|Public golf course
Rolling Hills Golf Club is an 18-hole regulation length golf course in Cleveland, Tennessee. This medium-length layout provides for a fun golf experience for golfers of all skill levels. Difficulty level of the course is unknown at this time, but will be provided soon. Online tee times may be available at Rolling Hills Golf Club or at nearby golf courses, often at a substantial discount from the going green fees rate.
|Course Rating
|Back Tees
|Course Superintendent: Wes Gilbert
|Manager: Dean Hale
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text: Add-to-cart for variable products, unlike simple products, makes the page reload, and a suffix (something like "?add-to-cart=variation&product_id=...") is appended into page URL, and, in my case, it's causing some conflicts with the pagination system. There's any way to solve it? Why this fragment is added to the URL? After adding to cart, I manually exclude it, reload the page, and everything works equally well (checkout, widgets...).


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"All the World is Welcome Here" ---Lyndon Baines Johnson
Lyndon B. Johnson National Historical Park tells the story of our 36th President beginning with his ancestors until his final resting place on his beloved LBJ Ranch. This entire "circle of life" gives the visitor a unique perspective into one of America's most noteworthy citizens by providing the most complete picture of any American president.Read More
Touring the LBJ Ranch
On August 27, 2008 the Texas White House officially opened to the public. Find out what you need to know if you are planning a trip to the LBJ Ranch.Read More
Boyhood Home Undergoing Restoration
President Johnson's boyhood home will be partially closed for tours for approximately 10 weeks, beginning July 13 and ending on September 25, 2015.Read More
Barbecue--LBJ Style
Join the Friends of LBJ National Historical Park on October 24 for a barbecue reminiscent of the ones President gave for guests to the LBJ Ranch.Read More
Trading Cards for Kids
Discover more than 500 stories about America's journey from the Civil War to Civil Rights. View online or visit this park to collect your own copies.Read More


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See definition in Oxford Advanced Learner's DictionarySaltos de línea: car¦il|lon
Pronunciación: /ˈkarɪljən/ /ˈkarɪlɒn/ /kəˈrɪljən/
Definición de carillon en inglés:
1A set of bells played using a keyboard or by an automatic mechanism similar to a piano roll.
Oraciones de ejemplo
- They visited many places of interest during their stay including the magnificent St Colman's Cathedral in Cobh which is constructed in a French Gothic style and has a carillon of 47 bells, one of the largest in these islands.
- The Laura Spelman Rockefeller Memorial Carillon is the second-largest carillon in the world.
- Cyril Johnston is credited as the man who rediscovered the art of bell tuning, an art form which had been lost for more than 200 years, and made the name of Gillett and Johnston synonomous with bells and carillons throughout the world.
1.1A tune played on a carillon.
Oraciones de ejemplo
- Handel makes delicious use of a carillon in the soprano aria 'Or let the merry bells ring round'.
- We look forward to a successful appeal, and hopefully in the not-too-distant future to hearing the melodious tones of the carillon once again.
- But in the end, the only bell that mattered was the noontime carillon that brought workers in for their one hearty meal at home.
- 1carillonneur
- Oraciones de ejemplo
- This instrument, with more than 100 tons of bronze bells and 72 English-made bells, is so captivating that the world's top carillonneurs come to perform on it.
- Other famous carillonneurs were Antoon Brees, Riverside Church of New York and Cranbrook Church in Detroit, and Camiel Lefevre of Bok Tower in Florida.
- The hour-long recitals on the cathedral's 49-bell carillon will be given by the resident and guest carillonneurs.
Pronunciación: /ˌkarɪljəˈnəː/ /ˌkaˈrɪlənəː/ /ˌkərɪləˈnəː/sustantivo
Late 18th century: from French, from Old French quarregnon 'peal of four bells', based on Latin quattuor 'four'.
Palabras que riman con carillonslumgullion
Definición de carillon en:
- Diccionario Inglés de EE.UU.
¿Qué te llama la atención de esta palabra o frase?
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Squirrel'S Nest Bed & Breakfast, LLC
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- Continental Breakfast
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- Wireless internet connection in public areas
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- Free High-Speed Internet
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FM Quick Update
... hours: open until people aren't there anymore. It's a cute spot: wine barrel tables, wine bottle light fixtures, photos/art representing the family winemaking history. It's the type of place I want to succeed. Also, the only beer on draught is Busch Light. Twelve ounces for $1.25. Hehehe...
Because I wasn't feeling 100%, I started with a sugary wine slushy - sweet concord ...
Back to the Gypsy that I was
Press PLAY
I got very comfortable in Minnesota over the past few weeks. A little too comfortable, perhaps, but that happens with friends like mine. Yet I was losing momentum on my travels and struggling to take the next step. Strange, but it's as though the approaching inevitability of the trip terminus took over my mind, bringing me down. It's entirely possible that I can't do this forever.
Driving back to Fort ...
Main Street, USA
... one for not having pie. It must be a big farmer hang out. There were a number of farmer type guys there who were friends with the waitress. One of them said he was ready to go and to have one more drink for the road. She filled styrofoam cup with Crown Royal and he was on his way. Crazy. Nothing like working on the farm with ...
Alright...today's joke comes from special guest contributor Tim:
Why does the Mississippi River flow south from Minnesota?
Because Iowa sucks
Iowa's response is best delivered by my mother's new horse
Lake Odessa (52 miles)
... existence. I included a picture so yall could partake. You're welcome. I couldn't get that smell off my mind. Maybe it was in the air outside as well. Everything was soaked and muddy. I haven't had to deal with taking down my tent in the rain. I've always been able to dry it before folding it away. As I took it down, it got completely soaked inside too. I just strapped it to the back rack on my bike ...