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15 votes
San Carlos Hot Harvest Nights-Farmers' Market
San Carlos, CA
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San Carlos Hot Harvest Nights-Farmers' Market
Kristen Atkinson
2260 days, 14 hours ago
I Love this market. Great vibes, great food, great products, great people.
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San Carlos Hot Harvest Nights-Farmers' Market
Kari Binley
2252 days, 22 hours ago
The market is full of great food and flowers but also helps support the local merchants. I enjoy buying my local food and then eating dinner at any of the fabulous restaurants that are around.
2258 days, 19 hours ago
The produce is truly fresh and the vendors are most helpful, At least the ones I shop from are very helpful. I agree with the other comments. I've been to some of the others in the area and look forward to Thursday afternoons.
2259 days, 23 minutes ago
Suzanne Lasseigne
2259 days, 28 minutes ago
It has a great selection of produce. It includes a booth on composting and vermicomposting. Included are live music and pets (kittens). Lots of people come.
Bob Dixon
2259 days, 10 hours ago
It has excellent quality produce, flowers, fish, music and other volunteer tables - a Master Composter table - everything you need to get excitied about organic food. Plus music, popcorn, Afghan foods, rotisserie chicken, free chiropractic massage and much more. Did I say friendly people?
Leila Parrott
2259 days, 21 hours ago
Great fresh produce, direct from our local farmers, combined with a wonderful sense of community. What could be better!?
Nancy Atkinson
2260 days, 13 hours ago
Fresh fruit and vegetable and lovely atmosphere
Kristen Atkinson
2260 days, 14 hours ago
I Love this market. Great vibes, great food, great products, great people.
linda richter
2266 days, 23 hours ago
Great vendors, bakers, chicken roasting, flowers. They have it all and on thursday afternoons! Perfect!
Christal Jensen
2274 days, 12 hours ago
Love to cook (and eat) and the produce couldn't be better. I can walk away with everything for dinner Thursday night and plan my menu for the next week around what's fresh. It's great to touch base with friends there, too.
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Identification of mature appressorium-enriched transcripts in Magnaporthe grisea, the rice blast fungus, using suppression subtractive hybridization
We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs, 72 have not been previously isolated in M. grisea. RT-PCR analysis of 105 of the genes discovered found transcripts corresponding to 71 of the ESTs only in mature appressoria while transcripts corresponding to a further 34 of the isolated ESTs were expressed both in appressoria and conidia/mycelia. Genes specifically expressed in appressorium identified by SSH included a number that have been previously implicated in appressorium formation or function including GAS1, GAS3, and PTH11.
- Appressorium
- cDNA
- Suppression subtractive hybridization
- Gene expression profile
- Rice blast fungus
1 Introduction
Rice blast disease, caused by Magnaporthe grisea, is one of the most severe diseases of rice throughout the world [1]. This fungal pathogen, as one of the best-studied species among phytopathogenic fungi, has been used as a primary model for elucidating various aspects of the host–pathogen interaction with its host [2⇓4]. This fungus as well a number of fungal plant pathogens can penetrate plant tissues by using the appressorium, a specialized cellular structure [5]. Appressorium formation appears to be a complex process from initiation to maturation [4⇓6].
Once attached to the rice surface, conidia of the fungus quickly germinate forming short germ tubes in 0.5–1.5 h. The germ tube then stops growing at the tip and a terminal "hooking" of hypha appears within 4 h. At approximately 4–8 h, melanization of the appressorium begins, followed by appearances of abundant glycogen rosettes and an appressorium pore ring within 16–24 h. At 24–31 h, glycogen rosettes are nearly absent, appressorial turgor pressure is at a peak, and penetration pegs emerge, infection hyphae form in 31 h and spread to adjacent epidermal cells in 48 h [6]. In view of the timing of the appressorium formation process, as outlined above, 24 h may be a key time point for production of turgor pressure and synthesis of the materials essential for the function of the M. grisea appressorium. Genes expressed during this stage may be required for appressorium maturation and/or penetration of the plant via the appressorium. For this reason we set out to identify genes expressed specifically in the late appressorium stage, but not expressed, or expressed at a low level, within conidia/mycelia.
Many approaches, such as expressed sequence tags (EST) sequencing [7⇓9], serial analysis of gene expression (SAGE) [10], microarray [11] and the isolation of appressorium defective or deficient mutants [12⇓14], have been used to identify appressorium specific genes. To date, several genes involved in fungal infection, specifically in appressorium formation and subsequent plant penetration, have been identified and characterized [4,15,16]. Despite these advances, the genetic basis of turgor generation within the appressoria of M. grisea is still poorly understood [17].
Suppression subtractive hybridization (SSH) technology is a powerful approach for identifying genes differentially expressed by cells or organisms in specific development stages or environmental conditions. For example, 35 transcripts showing a significant increase in expression during early stages of germination in Pyrenophora teres[18] and 12 genes whose transcripts are significantly enriched in Aspergillus nidulans conidia [19] were identified by SSH. A late appressorium cDNA library of rice blast fungus had been constructed in our lab [20]. Here, we report the construction of a subtractive suppressive cDNA library and discovery of transcripts uniquely expressed during appressorium maturation.
2 Materials and methods
2.1 Fungal cultures
M. grisea GUY-11 was used in this study. Conidia were obtained by harvesting plate cultures of the fungus grown on complete medium [21] in a 14-h-light and 10-h-dark cycle at 25 °C for 12–14 days. Conidia were harvested by scraping sporulating cultures with a glass rod in sterile distilled water, followed by centrifugation at 1000g for 10 min and resuspension in distilled water to a concentration of 1–1.5 × 106 spores/ml. Drops (20 μl) of this standard inoculum were applied onto the hydrophobic surface of projection transparency film (terylene resin; Gaoke, China) and incubated at 25 °C for 24 h. M. grisea aerial mycelia were obtained using the method of Talbot et al. [21]. Substrate mycelia of M. grisea were harvested by collecting cultures of the fungus grown in liquid complete medium at 25 °C with shaking at 180 rpm for 3 days.
2.2 RNA isolation and cDNA synthesis
Equal weights of conidia, aerial mycelia and substrate mycelia were mixed and quickly frozen in liquid N2 for RNA extraction. Total RNA of the above mixture and the appressoria harvested from duplicate films was isolated separately by a Trizol method following the manufacturer's procedure (Molecular Research Center, Inc., USA). Three microlitres of total RNA (?15 μg total RNA for conidia/mycelia mixture and ?5 μg total RNA for appressoria) was used for the synthesis of double-stranded cDNA with a SMART cDNA library construction kit (Clontech, USA). The protocol for SMART cDNA synthesis by LD PCR supplied with the kit was followed, until the proteinase K digestion step.
2.3 cDNA subtraction and cloning
For a cDNA library of genes specially expressed during appressorium stage, cDNA subtractive hybridization was carried out by following the protocol of the PCR-select cDNA subtraction kit (Clontech, USA) except for the use of AluI digestion of cDNA instead of RsaI digestion. The subtracted cDNAs were cloned into the pBlueScript II SK (+) Vector (Stratagene, USA).
2.4 DNA sequencing and analysis
The recombinant cDNA clones were directly sequenced on an ABI 377 DNA sequencer (Applied Biosystems, USA) with T7 primer. The sequence data were analyzed using VecScreen program (NCBI) for vector masking, and then the adaptor sequences (adaptor 1 and adaptor 2R) were removed from these cDNA sequences. These EST sequences were processed using software BioEdit [22] for contig assembly. Processed sequences were subjected to similarity searches against M. grisea database (genome ver. 2) (http://www.broad.mit.edu/cgi-bin/annotation/magnaporthe/) using Blast version 2.2.1 [23]. Once the genomic regions corresponding to EST sequences were identified, a representative EST sequence was selected for each gene identified by one or more detached EST sequence(s). All representative EST sequences were subjected to blastn and blastx search against GenBank database using Blast 2.2.8 [23] and against phytopathogenic fungi and Oomycete EST database (version 1.4) in COGEME (http://cogeme.ex.ac.uk/index.html) using Blast 2.2.5.
2.5 RT-PCR analysis
For detection of differential expression by RT-PCR, 3 μl of total RNA (?15 μg RNA for conidia/mycelia mixture and ?5 μg RNA for appressoria) was reverse-transcribed into first-strand cDNA by the protocol for SMART cDNA synthesis using SMART cDNA library construction kit (Clontech, USA). The primer sequences of RT-PCR used to detect mRNAs for the 105 clones were designed according to the EST sequences or predicted protein-coding sequences in M. grisea database (genome ver. 2) (http://www.broad.mit.edu/cgi-bin/annotation/magnaporthe/). After the initial denaturation (2 min at 94 °C), PCR was run for 35 cycles of 60 s at 94 °C, 30 s at 55 °C and 60 s at 72 °C. PCR products were separated on a 1.2% agarose/EtBr gel.
3 Results
3.1 Construction of subtractive cDNA library
Using microscopic assessment it was found that after ?24 h of incubation, melanized appressoria had developed in most of cells. The ratio of appressorium formation (appressoria numbers/conidia numbers) on the hydrophobic surface of duplicate film was 96%.
Total RNA of the conidia/mycelia mixture and the mature appressoria, incubated for 23.5–24.5 h on duplicate films, was isolated separately by a Trizol method. RNA quality was assessed using electrophoretic analysis which indicated that the total RNA was intact and suitable for use. From this RNA, a subtractive appressoria cDNA library, subtracted by conidia, aerial mycelia, and substrate mycelia mixture, was constructed. A total of 338 recombinant cDNA clones, from the subtracted cDNA mixture ligated to vector, were stored in −20 °C.
3.2 Sequence analysis
Among 338 subtractive cDNA clones, 250 clones were partially sequenced using T7 primer, and 155 non-redundant ESTs were generated from these cDNA sequences using BioEdit software [22]. Theses 155 sequences were compared to the draft sequences of the M. grisea genome ver. 2 (http://www.broad.mit.edu/cgi-bin/annotation/magnaporthe/), using the Blast algorithm [23]. 119 contigs, matching 152 ESTs were identified within the M. grisea database (Broad Institute), while no matching contigs could be found for the remaining three ESTs (Table 1). Some non-redundant ESTs, which were classed as unique by alignment among the ESTs, were found to match the same predicted M. grisea gene. This analysis revealed a match to a total of 139 different predicted genes from the M. grisea draft genome annotation. Assuming that the three ESTs which did not match any sequence present in the M. grisea database represent a further three separate genes absent form the draft genome sequence, a total of 142 genes in total have been shown to be expressed within maturing appressorium here.
|152a (139b)
a119 Contigs matching 152 ESTs were identified.
bThe values in parenthese indicate the number of genes that could be identified by comparing the EST sequences with the predicted gene sequences from 119 contigs.
A summary of homology search against GenBank using the Blast algorithm [23] and analysis of 142 cDNA sequences, representing 142 unique genes, is shown in Table 2. The source organism of every matching record of all homology searches in GenBank using blastn were checked and counted, and it was found that, among 142 ESTs, 70 ESTs have been correspond to known genes or have been previously sequenced in M. grisea while a further 72 ESTs have been isolated for the first time in the current study. Eight-nine per cent (when compared with protein databases) of the 142 ESTs had significant matches (p < 0.001) to known (or predicted) genes present in GenBank at the time of submission.
|E-3 to e-29
Among the 142 genes identified in 250 cDNA clones, the ESTs of 40 genes (28%) were detected at least twice. The most abundant 10 ESTs are listed in Table 3. Among these, the most frequently detected EST (16 times, 6.4%) and a further four ESTs show significant similarity to previously characterized genes or genes whose predicted product can be functionally categorized based on homology to known proteins. An EST matching probable keto acyl reductase encoding gene, involved in fatty acid metabolism, was detected nine times while ESTs matching the GAS1 (MAS3) gene, encoding a suspected virulence factor, were also detected seven times.
|Putative gene products
|Number of clones
|ESTs119 (CK828201)
|Predicted protein (MG02778.4)b
|16 (6.4%)c
|ESTs155 (CK828227)
|Probable keto acyl reductase (MG10351.4)
|9 (3.6%)
|ESTs98 (CK828185)
|Membrane-associated or secreted protein (MG02884.4)
|7 (2.8%)
|ESTs105 (CK828190)
|Predicted protein (MG08526.4)
|7 (2.8%)
|ESTs56 (CK828275)
|MAS3 protein (MG07044.4)
|7 (2.8%)
|ESTs9 (CK828294)
|Cytochrome P-450-alk1 (MG06973.4)
|6 (2.4%)
|ESTs28 (CK828256)
|Predicted protein (MG02287.4)
|6 (2.4%)
|ESTs134 (CK828214)
|Predicted protein (MG10355.4)
|6 (2.4%)
|ESTs19 (CK828247)
|Predicted protein (MG10345.4)
|5 (2.0%)
|ESTs127 (CK828209)
|Neuronal calcium sensor (MG01550.4)
|4 (1.6%)
aSequences were assembled from 250 ESTs randomly sequenced from a cDNA library constructed from appressorium cDNA (incubating for 23.5–24.5 h on an inductive surface) subtracted by cDNA of conidia/mycelia in PCR-select cDNA subtractive hybridization method. GenBank Accession Nos. are in parentheses.
bGene names in Magnaporthe grisea database (Broad Institute) that correspond to ESTs are in parentheses.
cPercentage in parentheses was calculated based on 250 cDNA clones analyzed.
3.3 RT-PCR analysis
To confirm differential gene expression in appressoria and conidia/aerial mycelia/substrate mycelia as indicted by SSH subtraction strategy, the transcript abundance of 105 of the genes identified was assessed using RT-PCR. Among 105 genes examined, 71 were expressed only in appressoria while 34 genes were expressed both in appressoria and conidia/mycelia (Table 4).
|Expression stages
|Appressoria, conidia/mycelia
|Gene numbers
Fig. 1 shows the gene expression analysis for nine genes in appressoria or in conidia/mycelia mixture of M. grisea. These genes, which include a predicted glyoxalase I-encoding gene (clone s126: GenBank Accession No. CK828208) and two ESTs predicted to encode a protein of unknown function (clone s134 and clone s119: GenBank Accession Nos. CK828214 and CK828201), seem to be expressed specifically in appressorium while the transcript is absent from conidia/mycelia. The EST matching the gene coding for the GAS1 (MAS3) protein (clone s56: GenBank Accession No. CK828275) was found expressed strongly in appressorium and very weakly in conidia/mycelia mixture. Two other genes, predicted to encode a product with no similarity to any known protein (clone s7: GenBank Accession No. CK828283) and a gene whose hypothetical product is related to acetyl coenzyme A synthetase (clone s117: GenBank Accession No. CK828240) seems to be expressed at a higher level within appressoria. The genes predicted to code for a predicted potassium channel beta chain (clone s20: GenBank Accession No. CK828252), an alcohol oxidase (clone s48: GenBank Accession No. CK828270) and a membrane-associated or secreted protein (clone s98: GenBank Accession No. CK828185) were highly expressed both in appressoria and conidia/mycelia. These results indicated that our SSH approach was successful in revealing differential gene expression during the maturation of M. grisea appressoria.
4 Discussion
Because the RNA obtained from appressoria was limited, the protocol for cDNA synthesis of mycelia/conidia and appressoria by LD PCR used in the construction of SSH subtracted cDNA library was different from the protocol provided by clontech PCR-select cDNA subtraction kit (Clontech, USA) but identical, with that used earlier in the construction of appressoria cDNA library [20]. According to the restriction enzyme cut site analysis of 8821 unique EST sequences of M. grisea obtained from COGEME 1.5 (http://cogeme.ex.ac.uk/), the number of AluI cut sites in the cDNAs synthesized with SMART cDNA library construction kit is nearly many as the number of RsaI cut sites in the cDNAs synthesized with clontech PCR-select cDNA subtraction kit (Table 5). If RsaI had been used to digest cDNA as suggested by cDNA subtraction kit (Clontech, USA), the subtracted cDNA library would lose its diversity greatly, because the cDNAs synthesized in this study only have 61%RsaI sites of the cDNAs synthesized by the protocol provided by clontech PCR-select cDNA subtraction kit (Clontech, USA). So, AluI was selected to digest cDNA. The analysis of subtracted cDNA library showed that good results were obtained using AluI digestion instead of RsaI. 155 independent clones were found among 250 recombinant cDNA clones and 71 gene transcripts were found to be unique to appressoria among the 105 genes examined with RT-PCR.
|Restriction enzymes
|Total number of sites in EST sequences
|Number of sites added by cDNA synthesis primera
|Number of sites added by CDS III/3′ PCR primerb
|Total number of sites in cDNAs synthesized by cDNA synthesis primerc
|Total number of sites in cDNAs synthesized by CDS III/3′ PCR primerd
|Rsa 1
aNumber of sites in cDNAs added by cDNA synthesis primer with clontech PCR-select cDNAsubtraction kit.
bNumber of sites in cDNAs added by CDS III/3′ PCR primer with SMART cDNA library construction kit.
cTotal number of sites in cDNAs synthesized by cDNA synthesis primer with clontech PCR-select cDNA subtraction kit.
dTotal number of sites in cDNAs synthesized by CDS III/3′ PCR primer with SMART cDNA library construction kit.
In the last decade, many scientific efforts have been focused on the molecular genetic basis of M. grisea pathogenesis. Analyses of subtracted cDNA libraries, SAGE and microarray have revealed a number of genes specially expressed during the early phase of appressorium formation in M. grisea or during the plant–fungus interaction stage [8⇓10]. However, the gene expression profile in the later stages of appressorium development has received very little attention until now. The fact that 72 ESTs (51%) of total 142 non-redundant ESTs identified from 250 recombinant cDNA clones in this study have not previously been isolated, suggests that many genes specific to the maturing appressorium remained to be discovered in M. grisea. The identification and characterization of these genes might provide important clues as to the probable metabolic pathways and/or structural features unique to the maturation of the appressorium.
In this study, we found ESTs matching many genes whose product is probably involved in lipid metabolism within peroxisomes. The predicted products of these genes include an acyl-CoA dehydrogenase (clone s104: GenBank Accession No. CK828189) , a probable keto acyl reductase (clone s155), an alcohol oxidase (clone s48), a glyoxalase I in pyruvate metabolism (clone s126), and two different kinds of acetyl-CoA synthetase involving in pyruvate dehydrogenase bypass (clones s283, s177: GenBank Accession Nos. CN121362, CK828241), four distinct P450 monooxygenases (clones s103, s116, s261, s9: GenBank Accession Nos. CK828188, CK828198, CN193452, CK828294), a D-2-hydroxy-acid dehydrogenase (clone s130: GenBank Accession No. CK828211), a further putative monooxygenase (clone s67: GenBank Accession No. CK828282) and a cyclohexanone monooxygenase (clone s159: GenBank Accession No. CK828229). The purified cyclohexanone monooxygenase is a remarkably versatile oxygenation catalyst that uses the bound flavin adenine dinucleotide (FAD)-4a-OOH oxygenating intermediate to initiate oxygen transfer to both electrophilic substrate sites, such as the carbonyl of ketones and aldehydes, and nucleophilic substrate sites [24]. It is likely that the expression of these genes plays a role in the function of the appressorium. At 24-h post germination the appressorium generates enormous turgor pressure (up to 8 MPa), which is used to rupture the plant cuticle [6]. The turgor inside the appressorium is generated by a rapid increase in intracellular glycerol levels, which is maintained by a specialized cell-wall layer containing melanin [25,26]. Peroxisomes are single-membrane-bound organelles possessing multiple metabolic functions, including β-oxidation of fatty acids, glyoxylate metabolism, and metabolism of reactive-oxygen species. Clapex6-deleted mutants of Colletotrichum lagenarium have a defect in fatty acid β-oxidation in peroxisomes and form small appressoria with severely reduced melanization that failed to produce infectious hyphae [27]. The gene ICL1 encoding isocitrate lyase, involved in the glyoxylate cycle, is required for full virulence by M. grisea and shows elevated expression during development of infection structures and cuticle penetration [28]. It has also been demonstrated that the mobilization and dissolution of glycogen and lipid is under the control of the PMK1 MAPK pathway and the CPKA/SUM1-encoded PKA [29]. Both of these signaling pathways are required for appressorium differentiation (give further references to the relevant papers here). An EST (clone s197: GenBank Accession No. CK828251) whose predicted product shows homology to a serine–threonine kinase Pdd7p, required for pexophagy in Pichia angusta and Hansenula polymorpha[30,31], was also identified in the current work. Taken together these results support the view that lipid metabolism plays an important role in infection biology, and suggest that a major metabolic activity during appressorium maturation is the conversion of fat reserves into glycerol. P450 monooxygenase genes, glyoxalase I and other genes could also be involved in secondary metabolism, especially in detoxification of antagonistic substances secreted by plants. Among these, the transcripts of a predicted glyoxalase I encoding gene (represented by EST clone s126), a probable keto acyl reductase (clone s155), three distinct P450 monooxygenase encoding genes (clones, s103, s116, S261), a D-2-hydroxy-acid dehydrogenase encoding gene homolog (clone s130) and a putative monooxygenase encoding gene (clone s67) were confirmed to be abundant within appressoria but were not detected within conidia/mycelia using RT-PCR.
Among the genes expressed in appressorium identified by SSH, some genes, including GAS1 (MAS3) (clone s56) and GAS2 (MAS1) (clone s266: GenBank Accession No. CN121353), THNR (tetrahydroxynaphthalene reductase, clone s142: GenBank Accession No. CK828220), and PTH11 (clone s247: GenBank Accession No. CN121346), have previously been reported to be involved in appressorium formation and pathogenicity [32⇓34]. GAS1 and GAS2 are preferentially expressed within appressoria in M. grisea compared with mycelial growth stage and although GAS1 and GAS2 deleted mutants have no defect in vegetative growth, conidiation, or appressoria formation, they are reduced in appressorial penetration and lesion development [34]. During the biosynthesis of fungal melanin, tetrahydroxynaphthalene reductase catalyzes the NADPH-dependent reduction of 1,3,6,8- tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8- trihydroxynaphthalene into (−)-vermelone [33]. Another pathogenicity gene, PTH11, encodes a transmembrane protein that is an upstream effector of appressorium differentiation in response to surface cues; strains lacking this gene are very reduced in pathogenicity due to a defect in appressorium differentiation [32]. Our RT-PCR analysis showed that transcripts matching GAS1 (clone s56), GAS2 (clone s266) and PTH11 (clone s247) were abundant with appressoria but scare within conidia and mycelia.
The predicted product of clones s248, s80 and s243 (GenBank Accession Nos. CN121347, CK828291, CN121345), have similarity to human oxysterol-binding protein, C14 sterol reductase and 6-phosphogluconate reductase, respectively. In C. gloeosporioides, a sterol glycosyl transferase gene (chip6) is induced by hard-surface treatment at an early stage of appressorium formation, and is required for fungal pathogenicity [35]. In M. grisea, clones AP2E02 and AP1I02, similar to phosphoglucose isomerase and delta-(24)-sterol c-methyl transferase gene involved in sterol biosynthesis, were found constitutively up-regulated during appressorium formation compared to vegetative mycelia [11]. These data are consistent with a role for sterol biosynthesis or sterol modification within pathogenesis by fungal phytopathogens [11,35].
Comparison of our data with results from previous analyses of subtracted cDNA libraries, SAGE and microarray in M. grisea[7,8,10] is of interest. Although some of the genes identified by previous analyses, such as GAS1, GAS2 and THNR, were also found in our study, most of the genes revealed by our experiments matched to predicted open/reading frames within the M. grisea genome sequence database for which no corresponding EST has been sequenced to date. The recovery of so many ESTs which have not been previously isolated in this species is likely due to the time point chosen. The gene expression pattern within mature appressoria after a 24 h incubation, is different from the pattern during the early phase of appressorium formation (after a 3–6 h incubation), a phase which has been much more extensively exploited in EST generation in M. grisea in previous studies (give some more references here). It is not surprising that we found many peroxisome-related genes, as that is the time when turgor pressure is generated from glycerol. Functional analyses of the novel genes identified during this work are underway in our laboratory.
The project was sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, partly supported by National Natural Science Foundation of China (Grant No. 30270049) and by Ministry of Science and Technology of China (863 Program No. 2002AA245041). We are indebted to the following for help and advice generously given to us during the planning and writing of this article: Dr. Muriel Viaud, Dr. Wei-Liang Chen and Professor De-Bao Li.
- © 2005 Federation of European Microbiological Societies

subset: commoncrawl


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15:24 Spain. The western extremity of the ancient world. Some suggest Paul thought of Spain as the Tarshish of Is. 66:19, and saw the extension of his preaching there as significant for the Christian mission (Matt. 24:14; Acts 1:8).


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With that being said, many athletes will sustain injury after injury, just to don their jersey each Friday night.
But when is enough, enough?
"I don't want to be paralyzed for life over one year of football. But as long as nothing happens, I want to keep playing."
Cameron Heard stands on the sidelines at Northwest Whitfield High School football practice. No helmet, no pads… he can only watch. He's benched for the season because of multiple injuries, including a concussion.
"After every hit, my head started hurting worse and worse. Eventually, everyone was telling me I was getting up slower and walking sideways. It just really hurt," said Cameron.
Cameron pushes past the pain to live up to family expectations. Both his brothers are former Bruins.
"I kind of have big shoes to fill, I guess. But I want to be better than them."
But Cameron is different.
He has Spinal Stenosis, or a narrow spinal canal, giving him higher risk of injury. His coach teaches several tactics to keep Cameron and others safe.
"When we're tackling, when we're doing anything, we tell our guys 'eyes to the sky,' because it locks your vertebrae in place and keeps you safe. Your eyes to the sky are a big deal. Anybody at any level of football will tell you that," said Head Coach, Josh Robinson.
Cameron's mother is a registered nurse. She understands the risks, but still allows Cameron to play.
"They're boys. They're going to get hurt whether they are playing football or basketball or climbing trees or riding bikes. So, I know there's a risk of him getting hurt. But boys are going to be boys and he loves football and basketball… so I let them play," said Kim Heard.
One Chattanooga neurologist agrees, many activities can cause concussions. However, he has seen one sport consistently send athletes to his office each fall.
"Clearly, football is and can be a violent sport," said Dr. Timothy Strait.
Dr. Strait says no medical testing can show evidence of a concussion.
"The diagnosis is made mostly by getting the story from the patient and people around that were eyewitnesses."
The biggest risk is Chronic Traumatic Encephalopathy, or CTE. Patients with CTE suffer from forgetfulness, depression, and sometimes, suicidal thoughts.
That risk is not confined to just college and the NFL. A recent study shows the G-Force of a hard hit to the head is the same for all players.
"But regardless, if you decide to play football, that's going to be something you may not be able to avoid," said Dr. Strait.
In his opinion...
"I would probably let my kids play football up until 5th or 6th grade, up through tag football. Then I might encourage them to play soccer or some other endeavor."
As for Cameron, his mom is on board, but they are waiting on the final opinion from the doctor.
Like most teens... he's living for today.
"I want to be with my team. I love my team. I love the bond we have between each other. They're like family."
Concussions usually take a few weeks to heal. Multiple concussions over time may never heal completely; which can lead to CTE and similar disorders.
It's important to keep in mind that football is not the only sport that can cause head injuries. Hockey, soccer, and many other sports can also lead to injury.
The bottom line for parents and kids is to have these discussions and decide what's best for their family.
By: Kelsey Bagwell
Is Football Glory Worth Potential Brain Damage?
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When the Pulitzer- and Tony Award-winning playwright Wendy Wasserstein — beloved for her plays "The Heidi Chronicles," "The Sisters Rosensweig" and "Isn't it Romantic?" — died in 2006 at age 55, Broadway dimmed its lights in her honor. Five years later, Julie...
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Don't forget to enter my Foster Farms giveaway!
Bushfire Kitchen is a cafeteria-style fast casual restaurant located in Carlsbad/La Costa. We recently were invited for a tasting, which we gladly accepted.
The restaurant is located in a large plaza, next to Vons. I really liked the little fountain right in front!
Bushfire Kitchen was opened by cousins Brandon and Clive, who are originally from South Africa and opened up the first location in Temecula. The original idea for the restaurant in Temecula was for people to be able to get a nice, wholesome meal for take-out – and eventually it extended into this cafeteria-style restaurant in La Costa. We met with Eddie, the manager, who was really nice and enthusiastic, and had a wonderful passion for food and his customers.
I also enjoyed the simple, rustic decor. The menu is listed on the board above the glass displays, which you can also find online here.
There was Batch Craft Soda, as well as beer and wines on tap. There were also house-made iced tea as well as hibiscus lemonade (not pictured). I tried the iced tea and sweetened it with a bit of agave syrup, which was quite good!
Eddie said that he would just send out some items for us to sample. The first thing to arrive was this beautiful platter of types of salads: mixed greens, Greek, kaleslaw, and quinoa. I enjoyed all of them, particularly the beets in the quinoa salad, and the kaleslaw, which I can honestly say is the best rendition of kaleslaw that I've ever had.
The salad was served with a house-made focaccia bread, which was amazing. It's served with a green onion dipping sauce, which worked really well with the bread – probably because it paired well with the nuts and seeds on the bread.
California Burger with Sweet Potato Fries
The California burger features a house-ground, organic and grass-fed beef patty, sharp cheddar cheese, arugula, avocado, and basil pesto aioli. …


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Trial begins for pope butler 'accomplice'
VATICAN CITY (AFP) – A Vatican computer technician accused of helping the pope's former butler leak secret memos went on trial on Monday, in a case which could expose other whistleblowers within the Holy See.
Claudio Sciarpelletti was being tried in the tiny state's 19th-century courtroom in what is expected to be a lightning trial as the Vatican rushes to wind up the embarrassing and damaging months-long scandal.
The 48-year-old is accused of aiding and abetting ex-butler Paolo Gabriele, who was sentenced to 18 months in jail last month after he admitted leaking papers alleging corruption and Machiavellian politics in the Vatican.
Cameramen and photographers have not been allowed into the courtroom for the trial and only a small group of journalists have been authorised to attend.
Vatican spokesman Ciro Benedettini told AFP the hearing had begun.
The technician was arrested on May 25 as the investigation into the leaks unfolded but only spent one night in a Vatican cell before being released.
A search by Vatican police unearthed a suspicious envelope addressed to Gabriele in Sciarpelletti's desk.
In it, they found photocopies of memos published by Italian investigative journalist Gianluigi Nuzzi in his book "His Holiness", which collected letters depicting intrigue and infighting inside the Vatican.
The book touched on everything from fraud allegations to sex abuse scandals.
Called to account, Sciarpelletti's testimony was confused and contradictory.
He said Gabriele had given him the envelope because he wanted his opinion on the contents, but that he had never opened it and had forgotten it was there.
He later said it was someone else entirely that had given him the envelope — a person identified in court documents only by the letter "W".
He also talked about a second envelope, given to him by a certain "X".
The trial will likely delve into the relationship between the butler and the technician. Gabriele — now serving time in a holding cell within the Vatican — is expected to take the stand to testify to their close friendship.
He was initially accused of giving false testimony, conspiracy to commit aggravated theft, aiding a thief and violating office secrets.
But the Vatican later reduced the charges, saying his alleged role in the leaks is "marginal" and that he faces only a light sentence if convicted after a trial likely to be shorter than Gabriele's, which lasted a week.
Other witnesses expected to be called include William Kloter, deputy commander of the Swiss Guard and Sciarpelletti's superior, Carlo Maria Polvani.
Monsignor Polvani is the nephew of Carlo Maria Vigano, formerly the Vatican's top administrator, who had written letters to the pope which were later leaked alleging corruption in the Holy See.
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Originally posted on HoopStuff | Last updated 9/18/13
Indiana Pacers: Pacers Free Agency Grade By Josh Morgan: Hoopstuff Senior Writer… Twitter @xjmo3406x * Moves can include signings, resignings and trades Pacers' moves: Resigned David West: 3 years, 36 million with a fourth year player option Signed C.J. Watson: 2 years, $4,122,720 Signed Chris Copeland: 2 years, $6.135 million Traded 2014 first round pick, Miles Plumlee and Gerald Green in exchange for Luis Scola Retaining David West was a no brainer for the front office. West was the main guy that brought the tough edge to Indiana and it was evident from his first game in blue and gold that the style of play was going to change from the free-wheeling, three point shooting under Jim O'Brien. The combination of West and Hibbert has been a great one, with them feeding off of one another incredibly well. He is a tenacious rebounder and defender to go along with his gritty offensive game. A variety of post moves are available to him, with a baby hook and a step back jumper being two fav...
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M/A-COM Technology Solutions (MACOM) will showcase a broad portfolio of new products for aerospace, defense and network applications, and present a technical paper during European Microwave Week in Nuremberg, Germany, October 6-11, 2013. Event attendees can visit MACOM at European Microwave Week in Nuremberg, Germany, October 6-11, 2013, Booth #169.
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Can you take plan b while brestfeeding
plan b while brestfeeding
Last edited 01/02/2013
Posted 01/02/2013
Do you happen to have a milk stash? Maybe take fenugreek to help keep your supply up. Also, if you have a pump, pumping throughout the day could prompt your body to increase supply.
Posted 01/02/2013
No. ok i will try that and see what happen, ugh hopefully that works
Posted 01/02/2013
Don't worry too much. I think it's only progestin, so it shouldn't affect it as much as estrogen. Just make sure your lo nurses a lot or pump. It should be fine and would only last a couple of days.
Posted 01/02/2013
Okay...! mmh should i tell my story so yall can help a lil more and why am asking this question or not
Posted 01/02/2013
Yes please tell your story.
EDD 9/18/2012....we welcomed our beautiful 6lb 4oz 21 1/4inch baby girl 9/5/2012
Married: 2-18-12
EDD 9/18/2012....we welcomed our beautiful 6lb 4oz 21 1/4inch baby girl 9/5/2012
Posted 01/02/2013
I would definitely talk to your OB about it though, it may have other effects. would your OB be open to even seeing if you ovulated via ultrasound since you are BFing? maybe you could avoid taking it at all if you didn't ovulate.
Posted 01/02/2013
Okay well on sunday my huusband and I had intercorse .He told me that there were no condoms; I said that in that case we shouldn't do it . My husband said well what if i pulled out nothing is going to happen , am like ok sure. He did but then at then end am i remember that the men can pre cum , so I got scare and told him that i shpuld get plan b he said alright just in case . But there is also another thing i havent had my menestrual cycle scince i had my lil girl ;she is now 3 months .ps am sorry for the typeos my phone is acting up. Let me know if u dont understand i will fix it
Your growing child
Your 3-year-old: Learning colors
Three-year-olds are beginning to learn colors. They can usually point to a color when asked and may be able to name a few. Read More
Your 3-year-old: Bed-wetting
Bed-wetting in potty-trained children Read More
Your 3-year-old: Little chatterbox
No more games of charades to decipher what your child wants, thanks to his improved diction and amazing grasp of grammar. Read More
Your 3-year-old: Friendships
Your ever-more-independent child is probably starting to develop some friendships. Read More


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Division Series - Boston Red Sox v Tampa Bay Rays - Game Three
Jose Molina of the Tampa Bay Rays reacts after striking out in the... News PhotoAmerican League Baseball,Baseball - Sport,Boston Red Sox,Facial Expression,Florida - US State,Game Three,Gulf Coast States,Jose Molina,MLB American League Division Series,National League Baseball,Sport,St. Petersburg - Florida,Strike Out,Tampa Bay Rays,Third Inning,Tropicana Field,USA,VerticalPhotographer Brian BlancoCollection: Getty Images Sport 2013 Getty ImagesST PETERSBURG, FL - OCTOBER 07: Jose Molina #28 of the Tampa Bay Rays reacts after striking out in the third inning against the Boston Red Sox during Game Three of the American League Division Series at Tropicana Field on October 7, 2013 in St Petersburg, Florida. (Photo by Brian Blanco/Getty Images)


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The collision happened Thursday afternoon near the intersection of Peru Hollow Road and Ohio 61, south of Norwalk.
To see pictures of the accident, click HERE.
One person was injured and taken to the hospital by ambulance. The injuries did not appear to be life-threatening.
More information will be published when it becomes available.


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KatanasPWN, on 14 January 2012 - 12:45 PM, said:
Have you actually tried making darts with the foam, or are you just checking if the raw foam fits? If it's the latter, make some darts with it, and see if they fit. Sometimes the foam changes when you put it through the drier, or however you straighten it.
I've got some blanks made up now that have been straightened, and they seem okay. I'm still hoping to find some higher quality foam that will fit the 17/32 breech though


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July 12, 2010
The University of Colorado Law School's Alumni Board announces the election of four new members to serve four-year terms. The 2010-11 Board Chair is Jan Steiert and the Chair-Elect is Kristin Rozansky. W. Harold "Sonny" Flowers, Jr.
July 7, 2010
Colorado Law School announces that after two years of study and discussion, the faculty has enacted an exciting new first-year curriculum reform, including a new required course in legislation and regulation and an elective course option during the second semester.A new course, Legislation and Re
June 11, 2010
Colorado Law has a new job opportunity: Program Director for the Public Sector & Student/Alumni Career NetworkingApply online at CU Jobs SiteThe Director reports directly to the Assistant Dean for Colorado Law School's Office of Career Development.
Professor Helen Norton was invited by the U.S. Senate Committee on Health, Education, Labor and Pensions to testify at a May 6 hearing to examine S.1756 (watch video), to amend the Age Discrimination in Employment Act of 1967 to clarify the appropriate standard of proof.
June 10, 2010
The Boulder County Bar Association awarded Professor Norm Aaronson the Ron Porter Award of Merit at a ceremony on June 9, 2010."I am very appreciative of this recognition by my colleagues in the Boulder Bar Association," said Professor Aaronson.
June 1, 2010
Colorado Law, in partnership with the University of Colorado at Boulder, installed new rooftop solar panels and an electrical car charging station in the Wolf Law Building, enhancing its reputation as a LEEDs certified building.The solar panels were installed and functioning in January 2010, whil
May 24, 2010
On May 18, Clyde O. Martz, a father of natural resource law in this country, passed away in New Mexico at the age of 89. He was an exemplary and memorable teacher, mentor, counselor, and advocate for clients, attorneys, and students throughout his long career.
May 20, 2010
Clinical Professor Colene Robinson received a 2010 Excellence in Practice Juvenile Law Attorney Award for guardian-ad-litems (GAL) from the Colorado Judicial Branch."It is an honor to be recognized as a child's attorney," said Professor Robinson. "I have the best job in the
May 18, 2010
Professor Pierre Schlag is the winner of Colorado Law's 2010 Jules Milstein Scholarship Award for his award-winning article is The Dedifferentiation Problem, 42 Continental Philosophy 35 (2009).The article demonstrates that our more sophisticated theories of law lead us to a point where we
May 10, 2010
Congratulations to the Class of 2010, the largest class, with 176 members, to graduate in Colorado Law history. After the traditional procession across the Boulder campus to Macky Auditorium, led by bagpipes, the faculty and administrators applauded the class as it entered the auditorium.
Wolf Law Building | 401 UCB2450 Kittredge Loop RoadBoulder, Colorado 80309303.492.8047Contact Us | ABA Required Disclosures


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Travelzoo members save 50% on tickets to Philadelphia's third annual Coffee and Tea Festival this November.Saturday, Nov. 7, from 10 a.m. to 5 p.m., and Sunday, Nov. 8, from 10 a.m. to 4 p.m.
Fall is a prime time to check out many of the city's food, art, and historic attractions.Select dates through December
Save 50% on fall and winter holiday stays at The Inn at Penn, a four-diamond hotel in the heart of U. Penn's tree-lined campus.Through Jan. 2, 2016
Dine on a "vast array of delicious buffet-style options" at Skybrunch, one of the "Top 5 Sky-High Vantage Points in Philadelphia" (Visit Philly), and save 40%.Through Dec. 27, 2015; Sundays only
As Bucks County foliage flames red and gold this autumn, steal away to Peddler's Village in New Hope with this charm-filled package that saves $140 on a one-bedroom suite with fireplace.Sept. 8, 2015 - Nov. 30, 2015
Enjoy a variety of wine-themed offers at Liberte Lounge, including wine flights with charcuterie for two, or admission to the French Wine Fair on Oct. 8.Through Nov. 16, 2015
"Experience luxury, privacy and soothing comfort as you pamper yourself like never before" (10Best) at Le Reve Rittenhouse Spa, and save over 30%.Through Dec. 16, 2015
Get a taste of all Philadelphia has to offer and save more than 45% on admission to the city's top attractions.Valid 9 days; redeem between March 1, 2015, and Feb. 29, 2016
See some of Downtown Philly's most historic landmarks by Segway, including the Liberty Bell and Independence Hall, and save 45% on your experience for two.Nov. 1 - March 31, 2016
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Save up to 40% on tickets when the show comes to Penn's Landing PlayhouseSelect dates through Nov. 7
Hotels with Member-Exclusive Extras
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Oldham County Adult Education offers free academic skill-building services for adults.
•Earn GED test credential
•Prepare for college
•Increase employability skills
•Assist kids with homework
For a limited time, pay only $10 for GED testing (per testing module).
Centre dean's list
Area students have been named to the dean's list for the spring term at Centre College, an honor reserved for students who maintain at least a 3.6 grade point average.
Students include:
•Benjamin Finch is the son of Cecilia and John Finch of Centerfield and is a graduate of Oldham County Senior High School.
•Hannah Ellis is the daughter of Scott and Jonna Ellis of Crestwood and is a graduate of South Oldham High School.
•Chandler Garland is the daughter of Michael and Jamie Garland of Crestwood and is a graduate of South Oldham High School.
Six Oldham County Schools students earned national honors this year through the National Merit Scholarship Programs.
From North Oldham High: Maggie Foster, Nicholas Landis and Hailey Simpson.
From South Oldham High: Caylem Ashcraft and Dylan Morris.
From Oldham County High: Reid Honeycutt.
Sydney Bright of South Oldham High School was elected as a delegate to attend the American Legion Auxiliary's (ALA) Girls Nation program in Washington, D.C.
ALA Girls Nation is a program for aspiring young women leaders providing firsthand experience and practical insight into how the federal government works. Two participants per state, known as "senators," are chosen to represent their respective Girls State programs.
Delany Young of Prospect and Sarah Abukwaik of Crestwood recently attended the Hugh O'Brian Youth Leadership Kentucky Seminar held at Eastern Kentucky University in Richmond from June 11-14.
OCEF appoints new members
The Oldham County Educational Foundation recently announced the appointment of three new board members who will begin serving in August.
Ryan Oliver, of Oliver Financial Group; Angelo Stekardis of UPS; and April Vance of Stantec will fill vacancies created by departing board members.
Kohl's Department Stores recently announced it has awarded more than 2,500 winners nationwide with nearly $400,000 in scholarships for higher education and prizes through the Kohl's Cares® Scholarship Program. The winners were chosen from more than 32,000 nominees nationwide, and selected for the positive impact their volunteer efforts have on the community.
Hendren VMI grad
The following Virginia Military Institute cadet
is among the 317 cadets who graduated May 16, 2015.
Oldham Co. Schools Board of Education
Students' fees were approved and there were a few changes from last year. The Business Courses fee of $20 will be removed. There will be a $23 fee added for English I-IV. The fee is not applicable for AP courses.
Kate Wetherton, of Crestwood, will represent Kentucky at the America's Natural Supreme Beauties National Pageant on June 11-14 in Louisville. Kate is the daughter of Brenden and Diane and a student at Locust Grove Elementary.
She was chosen as Grand Supreme KY Beauties and will represent Kentucky at the national pageant. Kate is a straight-A Honor Roll student and a member of the Duke University Talent Program.
Copyright © 2015
The Oldham Era is your source for local news, sports, events, and information in Oldham County and LaGrange, Kentucky, and the surrounding area.


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The health reform proposals being considered in both houses may impose responsibilities on both individuals and employers to have, and help pay for, coverage.
Subsidies for some small businesses and for individuals with incomes up to 400 percent of the federal poverty level have been proposed. But will individuals and businesses be able to pay the amounts required of them above the subsidies? If those costs are onerous, Congress may exempt many people from the coverage requirement or significantly reduce the penalties for noncompliance.
If that occurs, will enough healthy individuals purchase new coverage to adequately spread risk? How generous will subsidies in the final reform package be? What costs – both premiums and out-of-pocket – will individuals and families face? How will small employers be able to afford coverage for their employees?
To address these questions and others, the Alliance for Health Reform and The Commonwealth Fund sponsored a November 20 briefing. Panelists were: Sara Collins of The Commonwealth Fund, DeAnn Friedholm of Consumers Union and Stuart Butler of the Heritage Foundation. Ed Howard of the Alliance and Rachel Nuzum of The Fund co-moderated.
(If you want to download one or more slides from these presentations, contact us at info@allhealth or click here for instructions.)
Read More
WASHINGTON, DC (Aug. 3) – Edward F. Howard, founding executive vice president and CEO of the Alliance for Health Reform in Washington, DC, will retire in March 2016. A nationwide search is underway for his successor.
Over the years, the Alliance has presented hundreds of seminars on Capitol Hill and elsewhere, has organized briefings for reporters around the country and has prepared dozens of issue briefs and "toolkits," as well as a series of highly-regarded sourcebooks for reporters on health policy topics.
Dr. Robert Graham, Chairman of the Board, noted that the search for a new CEO is being coordinated by Association Strategies of Alexandria, Va. Those interested in learning more about this opportunity should email email@example.com or call 703/ 683-0580. You can also view the position profile on their website. The deadline for applications is Monday, October 12.
Read MorePosition Profile
The Alliance for Health Reform has released a new toolkit, "Biosimilars: Unpacking Complex Issues."
The Affordable Care Act created an expedited licensure pathway for biosimilars, and, in March 2015, the U.S. approved the first biosimilar, leaving policy makers, regulators, providers and stakeholders to grapple with regulatory and financial questions.
Biosimilars are similar – but not identical – to biologic drugs, and cost less. Unlike traditional pharmaceuticals, biologic drugs are derived from living organisms and tissues, making them more complex and expensive to produce.


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With 1.7 million freelancers, we'll match you with the perfect talent.
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I have a UK version of a business card (INdesign CS6) and I need a Mandarin Version. I have the text already written as mandarin and need it made in INdesign and sent back to me in the workable file. If it is not possible to send workable file then I will possibly need up to 2 sets of revisions.
This is 4 lines of text to be written as mandarin - everything else is done.
Please bid if you can do this today.
Added 19 NOV 2012, 6:35 AM EST
I need to add - This card is already designed - I do NOT need design. I only need some Mandarin text added to my existing design.
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Toronto newborn photography at it's best. Giving you creative and precious images.
Nationally Accredited in Newborn, Child & Infant Portraiture
There are some people that when you meet you just connect. P and her family are one of those people. Funny, sweet andView full post »
Keep reading and you're going to see some serious cuteness!! Mr. { A } is 4 months old…2 months adjustedView full post »
Sometimes, I don't just photograph strangers…sometimes, I'm also lucky enough to photograph family tooView full post »
This little one decided that he couldn't wait any longer and just wanted to surprise everyone by comingView full post »
I was very privileged to meet sweet little Miss { V } and her parents when she decided to come a bit early! This sweetView full post »
I was very lucky to meet these two-albeit briefly-a few days after they were born, in the NICU. These little munchkinsView full post »
I was lucky enough to meet { L } and her daughter at a Mommy Connections class. I had been invited to talk to a group ofView full post »
I got to meet a very special little guy yesterday. Mr. { R } is turning ONE and the family decided to have a cake smashView full post »
Being pregnant is such a special time in a woman's life. Knowing that you are carrying a little soul safe insideView full post »
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Philosophy Programs
What is Philosophy?
The term "philosophy" translates to "love of wisdom." People use philosophical lessons, beliefs and theories in their search to understand fundamental truths about themselves, the world in which they live, as well as their relationship to each other and to God.
Some of the questions that have become the objects of philosophical programs include:
- What really exists?
- Can anything be known?
- Are there any universal moral standards?
- Does God exist?
People with intellectual curiosity, who tend to ask themselves "Why?" and feel a need to find the answers to complex questions, enjoy and flourish in philosophy programs.
What makes Walsh's Philosophy major unique?
- Rooted in tradition. Our major is designed around the institutional commitment to Catholic values and beliefs. Consequently, our major emphasizes the Catholic identity of Walsh University.
- Two areas of concentration. We have two tracks of study: Great Ideas, which explores the great philosophical traditions, and Applied Ethics, which is designed to help students address moral values across disciplinary boundaries.
In addition, students have the option to enroll in Walsh's new 4+1 Program for non-business majors who would like to supplement their bachelor's degree with an MBA. Through the program, students simultaneously earn undergraduate and graduate credit for Walsh's MBA Prep Series courses, saving both time and money as students pursue their advanced degree.
Generally speaking, what courses will I need to take?
Philosophy majors need to take the Heritage courses required of all Walsh graduates. In addition, Philosophy students take gateway courses in the major. These are classes that enhance students' reasoning skills; research methods and writing styles that are used in this field; moral development; and understanding of Catholic tradition. Finally, students complete course work in their chosen concentration - either Applied Ethics or Great Ideas.


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iPhone 6S review
The new iPhone presents subtle upgrades such as 3D Touch, an improved camera and faster processor. Do they combine to make for a must-have upgrade?
The new iPhone presents subtle upgrades such as 3D Touch, an improved camera and faster processor. Do they combine to make for a must-have upgrade?
Seen as part artist, part visionary, the question facing Twitter co-founder Jack Dorsey now is whether he can turn the company around.
CEO Tim Cook, board member Andrew Jung, iTunes head Eddy Cue and others remember Apple's co-founder in messages posted to the company's internal site. Including the time Jobs mooned Al Gore.
Expected to arrive in late 2015, the latest update to Android promises great new features and enhancements.
The malicious software, called YiSpecter, hijacks apps and the Safari browser to show full-page ads. It fools users into installing it by claiming to circumvent China's Web censorship.
One key factor determines how long your food stays fresh (and how much you end up wasting).
This connected cam can follow the action with it's rotating head, and will learn your voice and respond to commands
Meet the latest iteration of the secure and privacy-centric Android-based smartphone.
14 minutes ago
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Google Plus SharesForget blades, the Skarp razor wants you to shave with lasers
MyithZ has one of the most popular aerial photography channels on YouTube. With the exception of revealing his identity, he is an open book as he shares with CNET's Brian Tong the drone hardware he uses to capture flyover shots of the construction of Apple's new campus, which looks remarkably like an alien craft.
The fall issue of CNET Magazine is here!
In the latest edition of our quarterly magazine, CNET highlights the latest innovations in your driveway and on the road.


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Events Calendar
- Wednesday, March 5, 2014
- Export2014-03-17T Blood Donation Opportunities
The Red Cross has Opportunities to donate Blood in many communities in Northeast Nebraska. Eligible donors with ALL blood types are needed, especially those with O negative, A negative and B negative. To learn more or make an appointment visit redcrossblood.org or call 800-733-2767.
- Export2014-03-30T10:00:00 Lapsit at Norfolk Library
10:00 am - The Norfolk Public Library offers "Lapsit" on Wednesday and Saturday mornings at 10:00 am as a beginning literary experience for children age birth to age 2 with an adult caregiver. Enjoy rhymes, stories, songs and games in English and Spanish with other children the same age. Call for details at 844-2100.
- Export2014-03-05T12:00:00 Nancy Gary Chapter DAR
12:00 - 3:00pm - The Nancy Gary Chapter of the D.A.R. will meet at the Norfolk Senior Center. Lunch at noon; meeting at 1:00. For more information, phone 402.371.0472
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This property allows you to book online with a credit card. After submitting a reservation request, the owner or manager has 24 hours to accept, at which time your card will be charged and you will receive an email confirmation
Recent Malibu reviews
"We stayed here with my family of 4 for a week in August. The place is exactly as described. All amenities in the unit for cooking etc, beach towels, chairs, umbrella, etc. The location is perfect. You can walk to almost anywhere. We always felt safe. The private parking directly under the building is very convenient and is steps to the front condo door. The pool is very nice, although we spent most of the time in the ocean. There is no A/C in this unit, but we did not need it at all during our stay. The condo is on a very private beach, so it is very quiet and relaxing, however you are just a short walk or drive to many of the area beaches if you wish. Make sure you venture into the Santa Monica mountains (Thousand Oaks and Calabasas) even if it is just for a burger at the In/Out Burger. The views are spectacular. Tonya was excellent to deal with. You could contact her by phone or email anytime and she was always available to answer any questions or address any issues or concerns. I would stay here again for sure and would recommend this place to anyone. Great experience booking with VRBO."Guest: Joe Date of Stay: August 2015 Review Submitted: September 1, 2015View listing
"The owner could not have been better..any questions etc were immediately answered. The place is much roomier than expected and very clean and comfy. My only negative which is beyond their control is if you are coming here expecting to have a beach right in front of your deck it's not there or at least not during the time we were..even during low tide for the four days we were there we never had sand to walk on, sit on, etc...several houses down there was enough to maybe put your chair on but that was about it...but it sounds like others were luckier..you can though hop in the car and within minutes have your choice of multiple, beautiful beaches. We ate lunch out everyday exploring the canyons,etc and saw some amazing views..we had breakfast in and whipped up some great dinners so for us regardless of the unavailable beach in front of us it was still perfect and the full ocean view off the deck was simply amazing!"Guest: jackie Date of Stay: September 2015 Review Submitted: September 23, 2015View listing
"My wife & I (+family) had an amazing time and loved your beach house. Thank you for allowing us to stay. With all the stress affiliated to planning & having a wedding it was a pure blessing to be able to take your mind off the madness & drift away w/ the waves. We'll be in touch the next time we're out in malibu. Cheers."Guest: Kristopher M. Date of Stay: June 2015 Review Submitted: July 8, 2015View listing
"Our stay was super fun, relaxing, and beat all our expectations! The home is clean and equipped with the essentials of a 5 star resort. Amber and Kyle are great home managers!! They made sure all our needs were met (Amber even had the linens cleaned to accommodate my allergies). We loved the private access to the beach, watching the sea lions from the hammock, walking the quiet small beach front, swimming and playing in the ocean. The sunsets, views from the deck of the ocean and the surrounding mountains were beautiful. The deck was especially nice to lounge and read on or just stargaze at night. We also loved the soothing sound of the crashing waves... We highly recommend staying in this remote beach home. We will be back!!"Guest: Lori L. Date of Stay: August 2015 Review Submitted: September 4, 2015View listing
Featured Destinations: Lake Almanor, Orlando Vacation Rentals, Big Bear Cabin Rentals, Anna Maria Island Rentals


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Slideshow: We Eat Everything at Feast Portland's Night Market
The scene
Crowds formed, but the most lines moved with relative ease.
Smallwares (Portland, OR)
Johanna Ware demonstrated the comforting qualities of noodle soup, updating the classic flavors with a heap of braised beef, whose intense flavors were balanced by pear kimchi, lemon, and sesame.
Bamboo Sushi (Portland, OR)
Jin Soo Yang gussied up a simple slice of fish with squid ink ponzu vinaigrette on kampachi sashimi. Black garlic salt added to the dark look, but micro chrysanthemum greens lent a tiny pop of color to the dish.
Aviary (Portland, OR)
Dark grill marks hid just how perfectly rare the chunks of beef in Aviary's wrap were, a testament to the skill of the chefs (Sarah Pliner, Jasper Shen, and Kat Whitehead). Pickled pears tucked into the lettuce wraps along with the meat provided a healthy crunch and blast of contrast.
Franklin Barbecue (Austin, TX)
People complained about the long line for Aaron Franklin's barbecue, but it sure beat the three-plus-hour waits at his Austin hotspot. Not to mention that they took their signature brisket up a notch for the evening, using Snake River Farms' American Wagyu beef.
The National (Athens, GA)
Bringing a little Southern flair to a Northwest event, Hugh Acheson's "rice grits," fell just to the thick side of a traditional congee, boosted with kimchi and topped with a resplendently marbled slice of American Wagyu beef ribeye.
North End Grill (New York, NY)
Floyd Cardoz got into the Northwest spirit, using the local specialty Dungeness crab to build a salad with avocados and grapes—a refreshingly light dish compared to many of his cohorts.
Bollywood Theater (Portland, OR)
Borrowing a street food specialty from Mysore, Troy Maclarty piled a rainbow of sliced tomatoes with puffed rice, Indian spices, and a cilantro sauce with a kick for his tomato churumuri.
Night + Market (Los Angeles, CA)
Armed with a most appropriate restaurant name for the event, chef Kris Yenbamroong turned out a simple, authentic version of sai krok Isan. The sour sausage from the Isan (Lao-Thai) people comes with a few flavorings as accompaniments: ginger, crispy shallots, peanuts, and hot peppers.
Zahav (Philadelphia, PA)
Michael Solomonov's beef basturma (air-dried beef, something like an Anatolian version of bresaola) served as the base for liver mousse sprinkled with crispy chicken skin bits called gribenes, and topped with pickled vegetables. The unique bite was one of the most surprising pops of flavor at the market, and one of the best dishes there.
Navarre (Portland, OR)
Navarre featured a ricotta and Parmesan cappeletti in brodo (a chicken/beef broth), along with polpetti (shown here), little meatballs that are a (delicious) byproduct of making the broth.
Incanto (San Francisco, CA)
"This is raw beef," chef Chris Cosentino had resentfully scrawled all over the table of his booth, as per health department instructions. The dish in question was a beef tongue tartare with "five shades of hay" (an assortment of microgreens), and teeny-tiny croutons.
Nong's Khao Man Gai (Portland, OR)
Nong Poonsukwattana and her crew worked as hard at entertaining their endless line with big smiles and goofy antics as they did wrapping up tiny packages of the local cart's signature—and rightfully famous—dish: tender free-range chicken, rice prepared in the broth from the chicken, and fermented ginger-soybean sauce.
Laughing Planet (Portland, OR)
With its location just feet away from the event, the (super) local spot offered two versions of coconut corn cakes—the chanterelle mushroom, roasted corn, black bean, and avocado cream one pictured above; and a braised pork version garnished with apple-daikon slaw and black beans.
Antique Taco (Chicago, IL)
Rick Ortiz's chili cheese curds were a little bit like that dream (nightmare?) where poutine sleeps with Frito pie. Tater-tot shaped fried cheese curds were buried under an avalanche of chili, avocado sauce, tortilla strips, and a few pickled peppers for heat.
Quin (Portland, OR)
Jami Curl, the creative mind behind Portland's renowned St. Cupcake bakery and Quin candy shop, brought caramelized honey seafoam with chocolate, marshmallow, and smoked cocoa salt.
Momofuku Milk Bar (New York, NY)
Christina Tosi transformed her famous cake into dense, rich, bite-size truffles in creative flavors: pretzel, chocolate chip-passion fruit, and birthday cake (shown here).