text:
Strand Hotel
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TripAdvisor Reviews Strand Hotel Bray
Travel Blogs from Bray
Drinking in Dublin
... the live entertainment.
Next stop was for dinner and after a bit of wandering we found an amazing restaurant where we spoiled ourselves to some fine dining and a great glass of red.
Feeling the day's drive and knowing that we had an early flight we curtailed our festivities and headed home, dreading what temperature our room might be.
Luckily the heater had started to do it's thing and with bag packed we headed to sleep excited for our next destination, ...
Travel to Ireland
... had a little Bailey's Chocolate Irish Cream and to bed. Wednesday, October 8: Early morning as bags were out at 7am and down to breakfast at 7am, ready to depart Dingle at 8:15am. Cloudy and drizzly day greeted us. Debbie and I were in the front seat today, behind Willy our driver. Today we are heading towards Ennis for one night. We stopped at Foynes Flying Boat Museum, to see original flying boat, stylus 314, which has been restored. Charles Lindbergh flew these as well as ...
Dublin to Waterford we go!
... we rented a car so we could make our journey down to Waterford, Ireland! Now just to be perfectly clear, the trip to Waterford was supposed to take a maximum of two hours, but for my grandparents & I driving for the first time on the "wrong side" of the road, it took us around 8 hours! We couldn't find the right motorway (highway) and got lost many times,but we eventually made it, after hitting a few curbs ...
A trilha do Mestre dos Magos | Dungeons & Dragons
... arrived in a beautiful town called Bray. Really had beach, with rocks, but until then had already adapted myself with the Croatia. But the wind and the cold was more for have some hot chocolate and cake. Even had some people taking a sun, clothing, but had. Gabi said we had to go up "on the cross", a mountaintop beachfront because it was worth and absolutely beautiful. I lokked Gabi and saw she was in boot heel (Anabella) and thought, should be a fast track and paved ...
Première journée - First day
... for more than 24h and with a jetlag of 5h and 8h. Other learnings of the day: - Egg mayonnaise is not Hellmann's. It's smashed eggs. No thanks! - There are still way too many smokers on patio. - The days are long. The sun came down at 11h30pm. I likey! - The Temple Bar pub, even though it's a landmark, it's easy to find, atmosphere, live band, and seats at the bar included. - Julie: "What's an Irish car bomb again?" Karine: "It tastes ...
Other places to stay in Bray
The Royal Hotel and Merrill Leisure ClubMain Street, Bray | 3 star hotelfrom $84
- A Pleasant Stay
- Customer Service Nil
- Faulty Towers
Esplanade HotelStrand Road, Bray | 3 star hotelfrom $78
- Fantastic Hotel
- North of Ireland visitor.
- Listen to your customers.
Wilton Hotel BraySouthern Cross Road, Bray | 3 star hotelfrom $79
Westbourne HotelQuinsboro Road, Bray | Hotelfrom $52


text:
Recent Updates
MTV edits travel nurse show after complaints, group says
26 November 2013
The final three episodes of MTV's controversial show on travel nurses, "Scrubbing In," are being re-edited to focus more on clinical scenes featuring nursing skills, according to The Truth About Nursing, a nonprofit that seeks to increase public understanding of nursing.
AMN acquires healthcare VMS provider ShiftWise
26 November 2013
ShiftWise, a healthcare vendor management system, was acquired on Nov. 20 by AMN Healthcare Inc. (NYSE: AHS), the largest U.S. healthcare staffing firm.
2013 Staffing Company Survey: Staffing firms' best and worst strategic decisions
26 November 2013
- Asked about their strategic or tactical decision making
- Staffing firms tell us their best decisions
- And their worst
The Buzz: An Old Dog Learns New Tricks
25 November 2013
Why LeoForce is a new breed of ATS.
The Other Side: Talk to Me
25 November 2013
How to prevent "analysis paralysis" in contingent staff.
3Q13 financial results for North America-based public staffing firms
25 November 2013
- Earnings up median 14.2% y/y, revenue up median 5.4%
- Signs of acceleration
- Revenue and earnings for 22 firms detailed
Power Seller: Embrace Change
25 November 2013
Alter your routine to get sales rolling — again!
Expert's Corner: Raising the Bar
25 November 2013
Tools to help you provide the best service to your clients.
Get the Basics Down: Then release upgrades to keep you strategic plan fresh, user friendly
25 November 2013
Strategic planning can be less difficult if you approach it like a tech firm.
The Eye of the Investor: Why private equity firms are interested in staffing
25 November 2013
Two investors share why the staffing industry is attracting more interest from private equity firms.

subset: commoncrawl


text:
The National Weather Service Office in Wakefield, Virginia has issued the following weather advisory for the North Carolina Counties of Bertie, Camden, Chowan, Currituck, Hertford, Gates, Pasquotank and Perquimans.
A Winter Weather Advisory remains in effect from Friday, January 25 at 1 pm through Saturday, January 26 at 1 am (local time).
The National Weather Service is predicting snow fall in the affected counties, mixing with or changing to all sleet near the Virginia / North Carolina border. Precipitation will be in the form of snow to sleet to freezing rain closer to the Albemarle Sound.
Accumulation of snow and sleet is predicted to be approximately 1 inch. Ice accumulation is predicted to be abot a tenth of an inch or less.
Temperatures will begin in the mid 30's and drop to around 30 later in the day.
The Weather Service predicts snow will develop during the afternoon and possibly change to sleet and freezing rain around 6 to 8 pm.
Drivers on area highways are warned that hazardhous driving conditions will develop later in the afternoon and persist into the evening as the snowfall accumulates and changes over to sleet and freezing rain. Drivers should be prepared for slippery roads and limited visibility.
NHC : HURRICANE EARL FORECAST/ADVISORY NUMBER 28 on September 1st, 2010
Fire damages Moyock home, resident sent to hospital on February 26th, 2013
Governor Announces Temporary Solution to NC 12 on September 2nd, 2011
Bomb Threats Phoned in On Two Local Banks on January 24th, 2009
Cancellations and Closings - January 25, 2013 on January 25th, 2013


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Wolves midfielder George Saville has joined Millwall on a 28-day loan.
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The squeals of delight could be heard down the hallways Feb. 1 at Chatfield Senior High School.
No, it wasn't because the Chargers girls basketball team was winning but rather because the future Poms girls in the stands were excited for their upcoming performance at halftime.
The kindergarten through 6th grade youngsters were part of the Chatfield Poms' fund-raiser – a three-day camp that culminated in a Friday night dance and cheer performance.
"For three days, the girls come for two hours after school and learn the dance, some cheers and learn technique," said Nicole Graham, the Chatfield Poms' coach. "We encourage the young girls to continue to take dance classes so they can be a Pom when they're in high school."
Graham participated in Poms herself when she attended Chatfield. She has been coaching the team for six years and wanted to keep the fund-raiser alive. That is why she became involved.
The camp costs $35 per child and includes a T-shirt, Pon Poms, admission to the game and a snack each day at camp.
The money goes toward Chatfield's trip to the National Dance Championship held in Orlando, Fla. This year, the competition will take place March 1 through March 3.
"This fund-raiser allows us to be able to go to nationals," said Kelsey Sturdevant, a senior on the Chatfield Poms squad. "This is a crucial fund-raiser because there are just a few ways to earn enough money for all of us to go. All of the elementary schools get involved ee Ute Meadows, Stony Creek, Bradford intermediate and primary and Coronado."
Sturdevant said the camp is something she looks forward to each year.
"I've done this for four years and this is my last year and it's kind of sad," Sturdevant said. "I've gotten more and more comfortable with the little girls and they're a lot of fun. It's always surprising how much they look up to us."
One of Sturdevant's teammates, Bryn Galisewski, is a junior who will return to Poms next year for her final year. Some of the camp's participants made her a card and came to give her "goodbye" hugs at the end of Friday's halftime show.
"Are you going to remember us?," one of the little girls asked while another seemed shocked that Galisewski was going to graduate next year.
"I love kids," Galisewski said. "This is like a dream come true for me. I love teaching dance and being around all of the little girls. "It's just great to see how they react to us and it makes me appreciate what I do. I am flattered that they look up to us."
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Results 6 to 7 of 7
Thread: A middle name for Charlotte?
April 24th, 2012 07:06 AM #6
Last edited by kyemsma; April 24th, 2012 at 07:08 AM.Anna Josephine ~ Caroline Grace ~ Catherine Violet
Elizabeth Pearl ~ Eve Charlotte ~ Jane Cordelia
Benjamin Arthur ~ Jack Ronan ~ James Frederick
Liam David ~ Luke Alexander ~ Patrick Elliot
April 24th, 2012 09:34 AM #8
My name is actually Charlotte My middle name is Josephine which I think sounds nice with Charlotte
Charlotte Lorelei
Charlotte Jane
Charlotte Eden
Charlotte Tessa
Sorry for any repeats!
Maybe you would want to give her a flowery/plant middle name like her sister's??
Charlotte Azalea
Charlotte Zinnia
Charlotte Flora
Charlotte Bryony
Charlotte Juniper
Charlotte Lily
I'm afraid I'm not very skilled in suggesting names, lol.
Good Luck!!
Last edited by midnightskies; April 24th, 2012 at 09:39 AM.Mommy to Derek Night (Born on August 31st, 2012) <3


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Registered and Licensed Dietitians are the most credible source of evidence based food and nutrition information. We provide Registered and Licensed Dietitians (R.D., L.D.) opportunities to enhance their knowledge and skills for success and leadership in practice, workplace and communities.
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Nominating Chair Theresa Johns, RDN, LD
Nominating Committee Kristen Decker
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Legislative Chair Theresa Johns, RDN, LD
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Hospitality Chair Joyce Fu, MS, RD, LD
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Summary: 6% of task attempts are extremely slow and constitute outliers in measured user performance. These sad incidents are caused by bad luck that designers can — and should — eradicate.
The UX Bookmark
- If you are a usability engineer, user researcher, human factors specialist, interaction designer or an information architect, the UX Bookmark is a resource specially for you.


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We need these labelsApril 17,2013
We need these labels
If there is one thing I passionately believe in, it is the relationship between human beings and their food; the substances by which we choose to nourish our bodies and spirits. Why, then, should we be concealed from the true composition of our food?
Companies have become consumed with profit-driven initiatives, debatably at the expense of peoples overall health and wellbeing. I fully lend my support to the GMO labeling bill that is currently under review (bill H.112) in the Vermont legislature because it fosters this most intimate relationship known to mankind. As the use of pesticides and other synthetic chemicals rise in conjunction with GMO crop production, we have all the more reason to be made aware of what it is in our food and how it has been produced. We might be a small state, but we are comprised of passionate and progressive thinkers who truly care about where their food comes from, how it is produced, and its affect on the body.
Take a stand. Voice your opinion. Let us be the first state in the nation to label genetically modified organisms in our food.
Stephanie Roque
Essex Junction
The writer is a student at University of Vermont studying for her Master of Science in Dietetics.MORE IN Letters
I am writing to express my support for the healthy workplaces bill, H. Full Story
I thank The Times Argus for the editorial "Guns and numbers" (Oct. Full Story
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Available for the first time in one volume, here is a complete translation from the original Latin of Domesday Book, together with an index of places and glossary of terms used. The detailed picture of the English landscape Domesday offers is unparalleled in any other country. It is valuable not just for the picture it allows local historians to reconstruct of their area as it was in the eleventh century, but also as the foundation document of the national archives. It has been used down the ages as evidence of title to land: remarkably, it was last consulted for legal precedent in 1982. "A must for scholars and history buffs everywhere!" - Michael Wood.
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This place has the nicest staff. In fact, I think Bellingham has the nicest people in the U.S. The only thing startling was the train that rode by but you get used to it. Otherwise, I recommend this place.
I didn't have time to take advantage of the spa, but I hear it's top notch.


text:
Pressure grows on Netanyahu to act with 'iron fist' in light of Palestinian terror onslaught
Netanyahu says Israel's relationship with Russia is good
Netanyahu attacked by right-wing flank for 'not doing enough' against terror
Netanyahu to Kerry: World must condemn Palestinian lionizing of terrorists
Tel Aviv's historic Great Synagogue has its eyes set on the future
Bon Jovi helps Israelis 'Keep the Faith' with empowering songs on a difficult night
Playing from the heart
Picturing change
Spotlight: Injured IDF officer Ziv Shilon goes the distance in inspirational Berlin marathon
Mac TA aims to cap perfect preseason in MSG finale
Mac TA back in first place, Mac Haifa finally off the mark
Maccabi Tel Aviv ready for Milano tests in Chicago, New York
Erekat: Recent outbreak in violence reminiscent of second intifada's early days
Liberman calls for tough measures to punish Palestinians for terror attacks
Special forces launch security, arms raids in Jenin
'Wide-scale operation may be needed to control Jerusalem terror'
Terrorist shot dead by police after stabbing youth in Jerusalem
British Conservatives want to block local councils from BDS activity
Jewish groups welcome announcement by Timmerman of EU anti-Semitism 'czar'
Bill Clinton pays tribute to Anne Frank
FBI destroys evidence linked to Medicare fraud among Orthodox Jewish community in New York
Criminal complaint filed against German Mayor for anti-Israel and anti-Jewish hate
US measure for Iran victims to get paid before sanctions waived inspired by Shurat Hadin
Analysis: The best reason for Iran deal? The West will learn where to drop bombs
Iranians launch 'Sorry Syria' campaign to apologize for Iranian jingoism
Obama and Zarif shake hands in New York, marking first such handshake in 30 years
WATCH: Russian warplanes bomb ISIS targets in Syria
'Russia can't claim to fight ISIS terror, while supporting Assad's terror'
Trump backs Russia, Iran efforts to fight ISIS
Only Assad truly fighting Islamic State, Putin says
US, allies short on options as Russia and Iran flex muscle in Syria
US weighs new support for Syrian rebels against ISIS
Obama: US will not make 'proxy war' with Russia over Syria
Health scan: Israeli physicians slowly warming to medical marijuana
Global space community to reach for the stars
Mazel Tov! Hebrew University places first in Israel in another school ranking
Beersheba students win top prize in Boston's Genetically Engineered Machine Competition
Ness Ziona residents threaten to sue Rehovot for raw sewage flow
Burgeoning alternative fuel firms in Israel grow fivefold in less than three years
IN PICTURES: 1,500 eagles pass over Israel on migration route
Green group demands swift action after sewage pollutes river, kills fish
Brewery producing craft beer made from Boston river water taps Israeli tech
El Al wins first place prize for startup program
Israel steady at 27th in WEF Competitiveness Index, ranks 3rd for innovation
Qatar, UAE lead Israel in broadband
GLOBAL AGENDA: Junk train wreck
Unpublished tour guide stories: Escapades in Acre
Meet the Ambassador: An ambassador at home in Israel
Grapevine: Diplomatic summit at the King David
Grapevine: What's on children's minds
Reb Yeivi's succa
Israel, the Jews and the European refugee crisis
Candidly Speaking: Jews, Islamophobia and compassion for refugees
Zahalka's hysterics
Everyone is 'fighting Islamic State' – and they're all lying
Letters to the Editor: October 4, 2015
Who is a hero?
The diplomacy profession
Coexistence in a cone
2016 Budget: Not just numbers
Voice in service
Socialist sentimentality
Working for Hitler
Armed with a sense of humor
Jaunt through Mount Zion
Serving Israel with love
Down to earth- At the Castel Museum
Life in a bell jar
Grapevine: My MK
Living in a shoebox
Rhythmic gymnastics on the rise
Shalev Ad-El lights up
מִשִירֵי רָחֵל: גַן נָעוּל
הַמֵיטָב שֶל אֲרִיאֵל זִילְבֶּר
יָבוֹא גְדִי זָהָב
נְמַל חֵיפָה זָכָה בַּמָקוֹם הָרִאשוֹן
Building health in Rwanda
'The Sabbath is on Saturday not Sunday'
Born to dance
Took a Hike
Classes for LGBT People Who Explore Converting to Judaism Open in Los Angeles
Silence is, sometimes, Golden
Teaching Biblical Hebrew
Celebrating death
Inauguration of the Masonic Forest Square in Jerusalem
Free Schach Distribution in KKL-JNF Forests
Remembering the Victims of 9/11
600 Holocaust Survivors Enjoy Touring with KKL-JNF
greenXchange at EXPO Milano
First Tree after Shmita Year Planted by Bavarian Agriculture Minister
Prime Minister of Lithuania Plants Tree in Jerusalem
KKL-JNF International Expo Mission to Milan
An Ecological Festival at the Shafir Winter Pond
KKL-JNF Global Landscapes Seminar at Expo 2015
New Bio-filters Purify Surface Runoff Water for Reuse
Future Tour Guides Discover the Negev with KKL-JNF
Got Water Problems?
$100 Million JNF Boruchin Israel Education Advocacy Center Opens
JNF Canada Pays Tribute to the Honourable John Baird
From Sunny Israel to Cold Canada
Renewable Energies for the Future in the Arava
LDA Conference: Science and Technology for Forests
The Negev Conference on Agricultural Research and Development
Arava Open Day: Food Security for Israel and the Entire World
Melinda and Chelsea: The Inspiring Story of Aliyah Across the Ages
An Olah Reports From Nepal
How One Oleh is Helping to Transform the Way People Drink Water
From Think Tank to Environmental Consultant
Why People Love Volunteering in Israel
How Natalie Edwards Built a Successful Career in Israel's Startup Industry
5 Tips to Land a Job in Israel
Starting Your Own Business in Israel: Real Life Examples
Where Are They Now? The Olim of the July 2014 Charter Flight
36 Hours in Southern Israel
Food Shopping In Israel
How Women Can Have it All in Israel
Why Retirees are Thriving in Israel
Thanks to Aliyah video
6 Frequently Asked Questions about Israeli Healthcare
Starting Over in Israel
Spotted on Social: Moshe Somers' Activity List
Sam Eisenberg: From Nativ to Aliyah
WATCH: Why Olim #LiveLoveIsrael
5 Ways that Olim Kick Back
WATCH: Highlights of 221 Olim Landing on the July 2015 Charter Flight
"WATCH: Highlights of 232 Olim Landing on the August 2015 Charter Flight"
Make yourself at home… in Israel
10.04.2015 | 21 Tishri, 5776
Prime minister to Channel 1: I'll be running again in next election
Not all bleak for Netanyahu
China's moves: US decline, or balance in Asia?
Hanegbi heads to Myanmar for highest-level visit in 35 years
Former ambassador to the US Oren may enter politics on list Kahlon is drafting
MK calls on PM to destroy homes of Jews who murdered Arab teen
Netanyahu: Hamas leaders are legitimate targets, no one is invincible
Kahlon indicates comeback with new 'framework'
'Israel no better than Chelm for providing electricity to Palestinian companies that boycott it'
Manhattan borough president shows solidarity with Israel visit
Ministers and MKs urge Netanyahu to pound Hamas
Right-wing MKs call to investigate Balad party, ban it from Knesset after Qatar trip
Diplomacy & Politics
02/03/2013 16:40
Ibrahim Sarsur and Ahmed Tibi at President's resident 370.
(photo credit:Marc Israel Sellem/The Jerusalem Post)
The Abraham Fund Initiatives called on Prime Minister Netanyahu late last week
to invite the Arab parties to join the government.The fund works to
improve the integration and equality of Arab citizens of Israel.A
statement published by the organization titled, "The Abraham Fund Initiatives to
Netanyahu" says that Arab citizens of Israel would like to see their
representatives integrated into the decisionmaking process and have Arab
politicians invited to join the government."The co-executive directors
of the Fund in Israel, Amnon Beeri- Sulitzeanu and Mohammad Darawshe, stated
that the invitation of the Arab parties to join the coalition has a value in
itself, because it conveys a positive message and encourages Arab
citizens.A meeting between Netanyahu and Arab leaders would allow them
to place important Arab issues on the agenda of the new
government.Research conducted by the fund before the elections showed
that Arab society yearns for real participation in the decision-making process
in Israel.More than 50 percent of respondents to the fund's survey said
it would increase their motivation to vote if the Jewish parties would
incorporate Arabs in running the country.The fund also wrote a letter on
January 30 to President Shimon Peres, echoing this message.Amnon
Beeri-Sulitzeanu, the coexecutive director of the Fund, told The Jerusalem Post,
"We think that Netanyahu needs to take the initiative and invite the Arab
parties to join the government [and] even if [the initiative] does not succeed,
he could still appoint an Arab MK as a minister in his government on a
professional basis, not a political one."He says Netanyahu could also
choose an Arab who is not a politician, but a professor for example, to serve as
health, transportation, or housing minister.Beeri-Sulitzeanu says that
"the government needs to promote Arabs to senior positions in the civil service
– to become for example, director general of various ministries. This period
after the elections is a good time to promote Arabs in the government and in
public service. We call on Netanyahu not to forget this while he is making his
coalition."In response to a comment that this initiative has little
chance of success, Beeri-Sulitzeanu responded that this is understood, and
mentioned that it is also quite possible that the Arab parties would not agree
to join for their own reasons. "But the point is that there should be a turning
to them, a symbolic approach." This also serves a
purpose.Beeri-Sulitzeanu emphasizes the importance of this as the
beginning of a dialogue and contact between them and the government, which is
what the Arab public wants. He says the Fund is aware of this because it
monitors Arab opinion."The question is, is it natural that 20 percent of
the population does not have a representative in the cabinet, does not have a
seat at the government table? This is also not good for Jews, not only Arabs."
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Barbecues (82 products)
1 - 50 of 82 items
When the British summer starts and ends within a short space of time you need to make sure you are prepared for those unplanned summer parties. Our barbecues, range from the tradition charcoal to modern gas fuelled ones. Nothing makes a sausage or burger taste nicer than when it's cooked on a barbecue in the summer sun, so make sure you've got one today.
Make sure you also stock up on the barbecue fuel, barbecue tools and barbecue covers to make sure you are fully equipped to look after your barbecue. After all, they're not like the disposable barbecues which can be thrown in the bin after each use: they're an investment you'll use for many summers to come!


text:
WindowPattern.CanMinimizeProperty Field
.NET Framework 4.6 and 4.5
Namespace: System.Windows.Automation
Identifies the CanMinimize property.
Assembly: UIAutomationClient (in UIAutomationClient.dll)
This identifier is used by applications. should use the equivalent field in WindowPatternIdentifiers.GTMTGTMT
In the following example, a root element is passed to a function that returns a collection of automation elements that are descendants of the root and satisfy a set of property conditions.
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.NET Framework
Available since 3.0
Available since 3.0


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Important delivery notice
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Our Spring Catalogue range brings you a fabulous selection of plants, trees, shrubs, vegetables and fruit to fill your gardens and features many new varieties for this year, including some world exclusives.
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Try calibrachoa plants alongside the more traditional petunia and you won't look back! Calibrachoa 'Ruby Buttons' is the perfect hanging basket variety. The trailing stems of this mini petunia are smothered in rich-ruby blooms right through the summer and their smaller size means they stand up better to weather extremes. A compact habit and dense flowering makes 'Ruby Buttons' a star addition to your summer container displays. Height and Spread 25cm (10").More info
Petunia 'Frenzy Pink' F1 Hybrid
Petunia 'Frenzy Blue Vein' F1 Hybrid
Petunia 'Frenzy Red' F1 Hybrid
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text:
Ash Wednesday: Rafa the gaffer is here to stay... so it's time for Chelsea fans to forget feeble protests and stand by their manager
Go on Chelsea fans, give Rafa a go.
When he walks out of the tunnel at Stamford Bridge before kick-off against Fulham this evening, show him some love.
Put your placards down, get up off your seats and break out into a spontaneous round of applause. Go on, do it.
VIDEO: Scroll down to watch Neil Ashton's preview of Chelsea v Fulham
Plenty to say: Chelsea manager Rafael Benitez speaks at a press conference at Cobham on Tuesday
Change of mentality: Benitez drills his message into the Chelsea squad on Tuesday morning
He's your manager for the next six months and no amount of banners or scribbled placards will alter Roman Abramovich's thinking.
On the scale of protests, it was
pretty tame stuff for the former Liverpool manager's first game against
Manchester City last Sunday.
There was the uncomfortable moment
when Chelsea's stadium announcer Neil Barnett introduced him to 40,000
fans and they responded with a resounding chorus of boos.
It wasn't pleasant, particularly when it continued long after he took his place in a spot previously reserved for Roberto Di Matteo.
A few banners were held up: 'Rafa Out, fact', 'Chelsea fans do not forget', 'In Roberto We Trusted and Loved. In Rafa We Will Never Trust. Fact!'.
High up in the West Stand, Abramovich, wearing a Champions League embossed bench-warming jacket, appeared unmoved. This is his manager for the next six months and Benitez has the owner's total support.
Work to do: The Spaniard has a chance to win five competitions this season with the Blues
Even when the stadium stood as one after 16 minutes to applaud Di Matteo and salute his achievements, Benitez did not buckle.
A proper protest by Chelsea fans would have left the stadium half-empty for the visit of the Premier League champions. Someone would have run on the pitch and ripped up their season ticket in front of a Sky TV camera and screamed 'No to Rafa the Gaffer'.
Instead they took their seats and watched Chelsea play out a depressing 0-0 draw against Roberto Mancini's team.
This is the time for Chelsea supporters to accept their fate and put some faith in the hands of Benitez.
Sure, he reacted to some of Jose
Mourinho's juicy grenades that were lobbed in his direction when the
Spanish coach in charge of Liverpool. Then they were just about equals, going head to head in those thrilling Champions League clashes between two top teams.
Benitez was always riled, but he had a right to defend his team, which is something he has reminded us ever since he was appointed by Chelsea.
This won't change anything: Chelsea fans make their feelings known at last Sunday's match against Man City
He cannot be faulted for standing his ground and taking Mourinho on when he was winding it up ahead of their Champions League semi-final in 2005, the group stages the following September and the 2007 semi-final.
Sure, it caused friction and tension between the teams, but Benitez is as entitled as anyone to bristle when Mourinho starts jabbing people in the ribs.
More from Neil Ashton for the Daily Mail...
- Chelsea manager Jose Mourinho stuns Blues stars with his brutal criticism 01/10/15
- Steve McClaren can't beat the language barrier as Newcastle United's cosmopolitan squad struggles to band together 24/09/15
- Emmanuel Adebayor was beginning to wind up Tottenham staff... chairman Daniel Levy is glad to be shot of the controversial striker 17/09/15
- Is brain train guru behind Gareth Bale, Joe Hart and Luke Shaw's mind blowing start to this season? 10/09/15
- Jimmy Greaves is one of football's genuine greats and his story must not be forgotten... but there are just five days left of an appeal to help care for the Tottenham legend 04/09/15
- Man City are ahead of the game just three years on from their first Premier League title... they have become Manchester's better-run club 03/09/15
- Southampton's talent are struggling to break through - is Ronald Koeman losing faith in the prolific St Mary's academy? 27/08/15
- Manchester United boss Louis van Gaal listens to his players... Memphis Depay's Champions League heroics are proof 20/08/15
- Tottenham chairman Daniel Levy called on Franco Baldini to close £10m deal for Lyon striker Clinton N'Jie 13/08/15
Benitez spent six years at Anfield and it is only natural that he protected the team and their interests. Those qualities should be admired and embraced by Chelsea's fans as they enter another uncertain phase in Abramovich era.
Time will tell whether or not Benitez is a brilliant manager, but for the moment he is the man leading Chelsea in five competitions.
They have a better chance in some than others, particularly in the FIFA Club World Cup where they will play a semi-final with either Ulsan Hyundai or Monterrey in Toyota, Japan, on December 13.
The final is at the International Stadium in Yokohama three days later when Chelsea are expected to go on to play Copa Libertadores champions Corinthians.
Success would help settle Benitez, bringing the silverware Abramovich demands of his managers back to the Bridge.
This is not an easy job, working for an owner who wants to wipe the floor with the opposition week in, week out. That is the vision, sending out a team who can dismantle the opposition and provide the ammunition for Fernando Torres.
For Benitez this is his shop window, a chance to show European football's top teams that he is worthy of employment in the summer.
He is a fascinating character, rivalling Arsene Wenger for his ability to accept the most pointed and prickly questions before and after matches.
It is a commendable quality in the modern game, but winning over the players with his methods is a priority.
Last Friday at Chelsea's training ground he gave the media yet another demonstration of the merits of zonal marking using a lucozade bottle, a bottle of Vittel and an empty glass.
Chelsea's players will be put through the same monotonous drills on the training field in the coming weeks as Benitez asserts his authority on the team.
He is determined to make the most of this unexpected opportunity and the changes to the mentality of Chelsea's players are already underway.
They appeared to be more cautious against City, but the expansive style that Abramovich demands will soon be put in practice.
He has replaced Di Matteo at a difficult time but whatever the circumstances of Benitez's appointment, he is part of Chelsea's history now.
For that reason, Chelsea's fans should get behind him.
- The PRICELESS reaction from Thierry Henry on Rodgers sacking
- Mourinho: It's up to the owners to decide if I leave Chelsea
- Floyd Mayweather and Justin Bieber relax on holiday together
- Brendan Rogers: 'I don't feel any pressure whatsoever'
- Wenger: Rodgers was a quality manager, I'm sad to see him go
- LVG: When you give Arsenal space you know you will lose
- Usain Bolt sings with Felix Neureuther at Oktoberfest
- Stuart Lancaster 'absolutely gutted' after loss to Australia
- Devastated Chris Robshaw speaks after Australia loss
- Defiant Jose insists Chelsea will be in the top four by Jan
- Jose Mourinho: There's no chance I'm running away from...
- 6,320 pull ups, 350 hours flying: Welcome to Rory's World
- Serena Williams shows off her pert posterior as she slips...
- Jurgen Klopp favourite for Liverpool job after Brendan...
- Watch Thierry Henry's comical reaction to Brendan Rodgers'...
- Brendan Rodgers says goodbye to Liverpool after being...
- Jurgen Klopp will demand final say on transfers if he is to...
- Liverpool sacked Brendan Rodgers BY PHONE after...
- Liverpool manager reaction: Jurgen Klopp, Carlo Ancelotti,...
- Arsenal boss Arsene Wenger on Brendan Rodgers' sacking at...
- Floyd Mayweather and Justin Bieber count their cash on...
- PSG 2-1 Marseille: Zlatan Ibrahimovic breaks club scoring...
- Jose Mourinho survives meeting of Chelsea board and will not...
- Ruben Loftus-Cheek to get extended run of games for Chelsea...


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Twitter used for social good and to incite disorder
Twitter and other social media sites are sparking an international debate over whether the forums serve the public good or make it easier to incite disorder.
Twitter and other social media sites are sparking an international debate over whether the forums serve the public good or make it easier to incite disorder.
Android smartphone users can take some commonsense precautions to protect their personal data from being stolen -- important advice considering an app developer purports to know how to take the information in under 60 seconds.
The digital watchdog Electronic Frontier Foundation (EFF) lent a technical hand to fix security problems in a tool used to encrypt instant messenger conversations using the Adium and Pidgin programs.
The latest espionage-related hacking campaign detailed by security vendor Trend Micro is most notable for the country it does not implicate: China.
The U.S. Federal Bureau of Investigation has arrested a Phoenix student, claiming that he is one of the LulzSec hackers responsible for a database attack on Sony Pictures computers that claimed more than 1 million victims.
When it comes to social engineering attacks, larger companies attract more of them, and when they are victimized it costs more per incident, according to a survey sponsored by Check Point.
As Netflix commits its future to streaming movies to customers, it relies almost exclusively on cloud services for its infrastructure, raising security concerns that require a new way of thinking, the company's cloud security architect says.
With the proliferation of data breaches, The Harford insurance company is selling a new data loss insurance aimed at small businesses that might be put at financial risk if they lose valuable and sensitive data.
Adobe on Wednesday patched six vulnerabilities in Flash Player, including one it admitted is already being exploited by attackers.
The latest social engineering trick to get victims to open malicious email attachments accuses them of being spammers and threatens to sue them if they don't stop.
The Dutch company that was hacked earlier this summer by certificate thieves has gone bust and shut down, its U.S.-based owner said Tuesday.
Japan's biggest defense contractor Mitsubishi Heavy has admitted that it was targeted by a major cyberattack last month in which at least 83 of its computers were infected with malware.
There's big trouble in the world of information security, and yet it seems that only a handful of us techies have noticed. What's the problem, you ask? Well, there are actually several problems, but they're all related to one very important issue: pu...
In November 2009 the European Parliament approved a directive on Internet privacy that, among other things, required user opt-in before websites could install cookies on the user's computer.
The theft of SSL certificates from Dutch certificate authority DigiNotar so undermined trust in the company that it has gone bankrupt.
The latest business news, reviews, features and whitepapers
Visit our shop for the best deals on hot products
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Comprehensive buying guides, features, and step-by-step articles
First impression on unpacking the Q702 test unit was the solid feel and clean, minimalist styling.
For work use, Microsoft Word and Excel programs pre-installed on the device are adequate for preparing short documents.
The Fujitsu LifeBook UH574 allowed for great mobility without being obnoxiously heavy or clunky. Its twelve hours of battery life did not disappoint.
The screen was particularly good. It is bright and visible from most angles, however heat is an issue, particularly around the Windows button on the front, and on the back where the battery housing is located.
My first impression after unboxing the Q702 is that it is a nice looking unit. Styling is somewhat minimalist but very effective. The tablet part, once detached, has a nice weight, and no buttons or switches are located in awkward or intrusive positions.


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NBA Offered To Drop $25K Fine Against Toronto Raptors If They Fired Drake
by Big Mo
posted Aug 14 2014 12:30AM
Drake once rapped "know your self, know your worth" and the Toronto Raptors just showed him what they think of his value. According to reports, the NBA offered to revoke the $25,000 "public recruiting" fine over Drake's actions if the Toronto Raptors let go of Drake. Last September, Drake was announced as the Toronto Raptors' "global ambassador", however his limitations and roles were not disclosed. The NBA fined the Toronto Raptors for $25,000, alleging that Drake publicly attempted to recruit Kevin Durant during his OVO Fest. Canadian news outlet The Globe and Mail reported yesterday (August 12th) that the NBA offered to revoke the fine if the Raptors relinquish Drake of his global ambassador title. The Raptors declined.
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The Loyola cross country season opened Saturday morning with the men's and women's team competing in the Annual Wolf Pack Invitational
The Loyola men and women's cross country teams will commence the 2012 season with their only home meet this Saturday at Lafreniere Park.
Loyola University New Orleans men's and women's cross country team took a break from running recently to stop and smell the roses, literally.
Outside hitters Eva Allen and Kailey Tuthill named to the University of St. Thomas All-Tournament team.
Loyola volleyball team set the mark for most victories to start a season as the Wolf Pack extended their streak to eight matches today
Loyola volleyball extended their win streak to six with a pair of wins on Friday
Loyola travels to Houston for a weekend tournament hosted by the University of St. Thomas.
Senior setter Sam Worsham's homecoming was perfect as the Wolf Pack volleyball team collected four victories on the weekend and the Chicago native earned MVP Honors at the Illinois Tech Tournament.
The Loyola volleyball team opened the season with a pair of victories at the Illinois Tech Tournament Friday.
The Loyola University New Orleans volleyball team will start the season on Friday at the Illinois Tech Tournament in Chicago. The Wolf Pack will open with Concordia at 1 p.m. and Calumet College of St. Joesph at 5 p.m.


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Roger Hodgson is the voice of Supertramp. Co-founding the band in 1969, he was the lead singer and writer of nearly all their hits throughout the band's heyday in the seventies and early eighties. Since leaving Supertramp in 1983 he has had a series of critically acclaimed and successful solo albums and come through a major accident in the late eighties that threatened his entire career. This DVD, the first live release from either Roger or Supertramp, was filmed in Montreal and catches Roger Hodgson at his very best.
Tracklisting: 1) Take The Long Way Home 2) Give A Little Bit 3) Lovers In The Wind 4) Hide In Your Shell 5) Oh Brother 6) The Logical Song 7) Easy Does It 8) Sister Moonshine 9) Love Is A Thousand Times 10) Breakfast In America 11) Don't Leave Me Now 12) Dreamer 13) It's Raining Again 14) School 15) Two Of Us 16) Give A Little Bit Bonus Tracks: 1) Even In The Quietest Moments 2) Dreamer (with orchestra) 3) The Logical Song (excerpt with orchestra) 4) Fool's Overture (excerpts with orchestra) Bonus Features: Bonus Tracks Behind The Scenes Film A Conversation With Roger Hodgson Montreal Interview Roger's Repertoire (full discography with audio clips)
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Fab video of the hits and faves! Not a bummer in any of the performances. He's really still got "that voice"!Published 5 months ago by Barry P. S.
This is truly a very fun concert to watch and listen to... as a former SuperTramp fan, this guy is the heart of SuperTramp.Published 6 months ago by Fred
I'm so glad I bought this DVD. Listening to it in 5.1 in my car with a 17 speaker system is a treat to the ears. Read morePublished 11 months ago by Sligo Lad
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|roger hodgson live in montreal
Sorry I don't have an answer for this. Did you ever find out what the answer was?
Oct 18, 2010 by J. Lupton | See all 3 posts


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Google Maps brings you 3D Landmarks Photo Tour
Google keeps improving its Maps by adding new features. Recently, Google has announced another improvement of Google Map – 3D models which displays around 15,000 landmarks and major buildings around the world.
It is very simple to operate the feature; just start a normal Google Maps search (such as type Colosseum, Piazza del Colosseo, Roma, Italy). If there is a photo tour showing in the results, you will see the photo on the left side of the screen. Simply click the image; you will fly into an animated, full-browser view of the destination. The 3D effect will appear, and rotate and change perspective as you move around the map.
However, to use this feature, your browser should be set to WebGL enable status.
The new 3D photos are sourced from Picasa and Panaramio. Google gathered all overlapping photos around the same locations, analyzed the 3D shape of the landmark, and calculated the location and orientation of each photo. It then proceeds to select 3D, animated paths through the best photos and your journey is ready to start.
In addition, Google listed all landmarks with this 3D features. If you are interested in traveling around the world and do not have enough time for the long trip, it might be a good idea to explore all listed landmarks first.
Going to explore Sydney Opera House now :)
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Legal Issues
Starting with the decision of how to structure a company, business owners are in constant need of sound legal advice. This section helps owners make sure they're getting good advice.
Essential Reading
Small-Business Guide
Intellectual Property
Establishing and protecting intellectual property rights is not beyond the reach of small entrepreneurs — in fact, it's essential.businessNews
Small-Business Guide
Managing an Online Reputation
Monitoring Web conversations, interacting with customers and honing your technical know-how can help your business get ahead.businessNews
A Political Message Amid the Movie Trailers
Moviegoers may get a dose of advocacy this month as commercials intended to spell out the perils of frivolous lawsuits come to theaters.businessNews
Toward Saner, More Effective Prison Sentences
A bipartisan reform bill is a crucial first step toward unwinding the government's reliance on prisons.
October 4, 2015, Sunday
Reparations in 1983 Beirut Bombing Among Cases Added by Supreme Court
The court agreed to add 13 cases to its docket, including one about payments from Iran to relatives of the 1983 Marine Corps barracks bombing in Lebanon.
October 2, 2015, Friday
Sentencing Overhaul Proposed in Senate With Bipartisan Backing
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October 2, 2015, Friday
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The judge, Jonathan Lippman, announced Thursday he would make administrative changes to reduce the number of people who are in jail because they cannot afford bail.
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Doug Kendall, Liberal Who Challenged Supreme Court's Conservatives, Dies at 51
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1965: Alabama Shooter Is Acquitted
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September 29, 2015, Tuesday
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Heacham - Heapham
A Topographical Dictionary of England
Heacham - Heapham Heacham (St. Mary) HEACHAM ( St. Mary), a parish, in the union of Docking, hundred of Smithdon, W. division of Norfolk, 14 miles (N. N. E.) from Lynn-Regis; containing 811...
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OS Map name 005/SE
Map of Norfolk
Heacham, PE31 7
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Subsidy Roll 1319: Portsoken ward
Two Early London Subsidy Rolls
241 f., Mem 174 ff.). - Heacham or Heigham Nf. Possibly Alexander Cobbe
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Archdeacons: Sudbury
Fasti Ecclesiae Anglicanae 1066-1300: Volume 2, Monastic Cathedrals (Northern and Southern Provinces)
to be identified with M. Reiner of Heacham (Norf.) (see Norfolk Portion
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House of Lords Journal Volume 36: February 1780 21-30
Journal of the House of Lords Volume 36, 1779-1783
the House of Peers; and to adjourn as they please. Heacham Enclosure Bill. ... Brecks, Commons and Wastes, within the Parish of Heacham, in the County
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Index: J
Calendar of Treasury Books, Volume 26, 1712
and searcher at Heacham and Brancaster in Wells port, 93. -, Joseph, master
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Gazetteer of Markets and Fairs in England and Wales To 1516
326100 Haveringland 1348 1348 59.25 615200 320900 Heacham 1272 1272 247.5
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Index: H, I
Calendar of Close Rolls, Henry VI: Volume 2, 1429-1435
Heywarde, John, 62. -, Walter, 180. Haywode. See Heywood. Heacham, Hecham, H. ... Hecham, Norfolk. See Heacham. Hecham by the sea. See Heacham. Hechyngton.
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Index: F
Calendar of Treasury Books, Volume 23, 1709
of Scotland, 472. Ford, John, appointment as boatman at Heacham and Brancaster,
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Index: H, I
Calendar of Treasury Books, Volume 23, 1709
Kent, 423. Heacham, Hechham, Hertham, Hictham, co. Norfolk, Customs ... collector at Colchester, arrears of, 443. Hechham. See Heacham. Hedges, Sir ... sheriff of. See Houblon, R. Hertham. See Heacham...
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Video: Mo. suspect strikes officer during violent club melee
Struggle between an intoxicated suspect and an officer is under review by the Springfield Police Department
By PoliceOne Staff
SPRINGFIELD, Mo. — A video capturing a struggle between an intoxicated suspect and an officer is under review by the Springfield Police Department.
According to the Springfield News-Leader, Officer Tomas Jordan and another officer were called to the scene to assist in getting "several combative people" out of a club on April 27.
Fearing for their safety as the crowd grew rowdy, officers called for backup.
It was then that Officer Tomas Jordan saw 24-year-old Courtney Lewis approach him and take a "fighting stance," according to a police statement reported by the Springfield News-Leader.
The video shows Lewis being pushed to the ground after approaching the officer. Lewis quickly stands up and begins throwing punches at Officer Jordan.
Lewis was charged with second-degree assault of a law enforcement officer. Officer Jordan suffered a scratch and swelling on his face.
The department is currently reviewing use of force in the incident.
Recommended Use of Force
Join the discussion
PoliceOne top 5
- 1 Dunkin' Donuts employee writes 'Black Lives Matter' on RI cop's coffee
- 2 Viral video: Armed Instagram user stalks Calif. cop
- 3 Racial profiling law is 'terrible' Calif. legislation, police officials say
- 4 Another Texas department adds 'In God We Trust' to patrol vehicles
- 5 Video: SC police shoot, kill man after he points gun at them in a pursuit


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An online journal about visual art, the urban landscape and design. Mary Louise Schumacher, the Journal Sentinel's art and architecture critic, leads the discussion and a community of writers contribute to the dialogue.
Design Ideas: Stacey Williams-Ng's chalk art
Chalk art may be considered a low art form by many. It's literally down on the ground with the dirt and cigarette butts. But people sure do look at it. »Read Full Blog Post
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Stacy's also great to follow at @staceywng
Saw her working on the piece on the Friday at the festival. Very nice! Pretty u'wear also!
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Page 1
Epithelial cell-targeted transgene expression enables isolation
of cyan fluorescent protein (CFP)-expressing prostate stem/
progenitor cells
Weidan Peng and Yunhua Bao
Lankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096, USA
Janet A. Sawicki
Lankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096, USA
Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia,
Kimmel Cancer Center, Jefferson Medical School, Thomas Jefferson University, Philadelphia, PA
To establish a method for efficient and relatively easy isolation of a cell population containing
epithelial prostate stem cells, we developed two transgenic mouse models, K5/CFP and K18/RFP.
In these models, promoters of the cytokeratin 5 (Krt5) and the cytokeratin 18 (Krt18) genes
regulate cyan and red fluorescent proteins (CFP and RFP), respectively. CFP and RFP reporter
protein fluorescence allows for visualization of K5+ and K18+ epithelial cells within the cellular
spatial context of the prostate gland and for their direct isolation by FACS. Using these models, it
is possible to test directly the stem cell properties of prostate epithelial cell populations that are
positively selected based on expression of cytoplasmic proteins, K5 and K18. After validating
appropriate expression of the K5/CFP and K18/RFP transgenes in the developing and adult
prostate, we demonstrate that a subset of CFP-expressing prostate cells exhibits stem cell
proliferation potential and differentiation capabilities. Then, using prostate cells sorted from
double transgenic mice (K5/CFP + K18/RFP), we compare RNA microarrays of sorted K5+K18+
basal and K5−K18+ luminal epithelial cells, and identify genes that are differentially expressed.
Several genes that are over-expressed in K5+ cells have previously been identified as potential
stem cell markers. These results suggest that FACS isolation of prostate cells from these mice
based on combining reporter gene fluorescence with expression of potential stem cell surface
marker proteins will yield populations of cells enriched for stem cells to a degree that has not been
attained by using cell surface markers alone.
Prostate; Adult stem/progenitor cells; Transgenic mice; Cytokeratins
© Springer Science+Business Media B.V. 2011
firstname.lastname@example.com .
Electronic supplementary material The online version of this article (doi:10.1007/s11248-010-9478-2) contains supplementary
material, which is available to authorized users.
NIH Public Access
Author Manuscript
Transgenic Res. Author manuscript; available in PMC 2012 October 1.
Published in final edited form as:
Transgenic Res. 2011 October ; 20(5): 1073–1086. doi:10.1007/s11248-010-9478-2.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 2
Normal prostate gland epithelium consists of two major epithelial cell types, basal cells and
secretory luminal cells. The lineage relationship of these cell-types has long been of interest.
Several years ago, a stem cell driven hierarchical mechanism for renewal of the epithelial
cells of the prostate was postulated (Bonkhoff et al. 1994; Isaacs 1987). As this model has
evolved, it is generally thought that a subset of the basal cells are the progenitor or stem
cells of the prostate, and that these stem cells give rise to basal cells which in turn give rise
to secretory luminal cells and neuroendocrine cells (De Marzo et al. 1998). Strong evidence
for this model derives from the fact that androgen independent basal cells that populate the
regressed prostate upon androgen withdrawal are capable of restoring the intact prostate
cellular structure upon androgen re-administration. The fact that cells having properties of
stem/progenitor cells have been isolated based on the expression of proteins expressed on
the cell surface of basal cells, including α2β1hi, ScaI, CD49f, CD44, CD133, CD117, and
Trop2, is additional support for the stem cell hierarchical mechanism for the development
and maintenance of the prostate epithelium (Goldstein et al. 2008; Lawson et al. 2007;
Leong et al. 2008; Richardson et al. 2004).
In contrast to these findings, a few studies suggest that basal cells are not essential for
prostate regeneration, and a small population of luminal cells may have stem/progenitor cell
properties. Urogenital sinus, the embryonic origin of the adult prostate, isolated from basal
cell marker p63 knockout mice can develop into prostate tissue containing only luminal and
neuroendocrine cells, but not basal cells, when grafted into adult male mice. The grafted
basal cell-devoid prostate can regress and regenerate in response to changes in androgen,
indicating that cells other than basal cells have stem/progenitor cell capabilities (Kurita et al.
2004). Cell kinetic studies following androgen-induced cell regeneration in the prostates of
castrated rats suggest that basal and secretory cells derive from two independent lineages,
thus providing further evidence that challenges the hierarchical stem cell theory (English et
al. 1987; Evans and Chandler 1987). A more recent study showed that a sub-population of
Nkx3.1-expressing luminal cells display stem/progenitor cell properties (Wang et al. 2009).
Given the unsettled state of our understanding of prostate stem cells and cell lineages and
the potential role that stem cells may play in cancer initiation, the isolation and
characterization of prostate stem/progenitor cells from the mouse prostate is of interest and
has been the focus of recent studies. These studies have relied on the concurrent use of
multiple antibodies to selected cell surface proteins for removal of non-epithelial and
differentiated epithelial cells from prostate cell preparations by fluorescent activated cell
sorting (FACS) (Goldstein et al. 2008; Lawson et al. 2007; Leong et al. 2008). While this
approach has yielded cell populations that are highly enriched for stem/progenitor cells,
technical limitations associated with antibody-based isolation have made it difficult to
achieve pure stem/progenitor cell populations.
Aiming to improve upon our ability to isolate a pure population of prostate stem/progenitor
cells, we have developed two transgenic mouse models, K5/CFP and K18/RFP, for efficient
and easy isolation of epithelial basal cells and luminal cells, respectively. In K5/CFP mice,
cyan fluorescent protein (CFP) is regulated by the promoter of the bovine cytokeratin 5 gene
(Krt5), while in K18/RFP mice the promoter of the murine cytokeratin 18 gene (Krt18)
regulates red fluorescent protein (RFP). Cytokeratin 5 (K5) and cytokeratin 18 (K18) are
cytoplasmic proteins; K5 is a conventional marker for basal cells of the prostatic epithelium,
while K18 is highly expressed in prostatic luminal cells. Fluorescence of CFP and RFP
reporter proteins allows for visualization of K5+ and K18+ fluorescing cells within the
cellular spatial context of the prostate gland and for their direct isolation by FACS.
Peng et al. Page 2
Transgenic Res. Author manuscript; available in PMC 2012 October 1.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 3
In this study, we subject FACS-isolated epithelial prostate cell populations to a number of
stringent immunostaining and functional biological tests and demonstrate that a
subpopulation of CFP+ cells has properties consistent with the presence of K5-expressing
stem cells in the basal compartment. A comparative analysis of the microarray gene activity
profiles of CFP+RFP+ and CFP−RFP+ FACS-isolated cells identified several putative stem
cell markers that are over-expressed in CFP-expressing cells as compared to CFP-non-
expressing cells, adding further support for the presence of a stem/progenitor cell population
in the basal compartment. These results suggest that FACS isolation of prostate cells from
K5/CFP mice based on combining CFP fluorescence with potential stem cell surface marker
protein expression will yield enriched stem cell populations to a degree that has not been
possible to attain using cell surface markers alone.
Materials and methods
DNA constructs
pK5/CFP was constructed as previously described (Peng et al. 2007). pK18/mRFP was
constructed using pCX/mRFP, a gift from Anna-Katerina Had-jantonakis (Memorial Sloan-
Kettering Cancer Center, New York) and pK18iresEGFP, a gift from Robert G. Oshima
(UCSD). Fragments of mRFP and IRES were amplified by PCR. The primers used in pK18/
mRFP cloning are: IRES 5′ GGTACCCCGCGGGCCCCTCTCCCTCCCCCCCC, IRES3′
CTCGGAGGAGGCCATTATTATCATCGTGTTTTTCAAAGGAAAACC, mRFP 5′
TTGAAAAACACGATGATAATAATGGCCTCCTCCGAGGACCTCATC, and mRFP 3′
NNNGCGGCCGCTTTAGGCGCCGGTGGAGTGGCGGCC. IRES 3′ end and mRFP 5′
end were overlapped. The PCR product was mixed and run five cycles without primers to
extend the overlapped part. Then, IRES 5′ primer and mRFP 3′ primer were added to
amplify IRESmRFP fragment. The PCR fragment was cloned into SacII and NotI digested
pK18iresEGFP to replace IRESEGFP.
Animals and preparation of prostate epithelial cells
K5/CFP transgenic mice were generated as previously described (Peng et al. 2007).
Following the breeding of K5/CFP heterozygotes to C57BL/6 J mice (Jackson Laboratory)
for more than 10 generations, heterozygotes were bred each other to generate a K5/CFP
homozygous transgenic line. To generate K18/RFP transgenic mice, pK18/mRFP was
digested with XbaI and NgoMIV and the 12 kb transgene fragment containing K18 and the
monomeric red fluorescent protein (mRFP) was purified and microinjected into nuclei of
B6C3F2 fertilized mouse oocytes as described (Hogan et al. 1986). Adult mice used in
immunostaining or cell sorting experiments were 8 weeks of age or older. Following
dissection of prostates under a dissecting microscope, prostate cell suspensions were
prepared as previously described (Sawicki and Rothman 2002).
Athymic nude mice and timed pregnant Sprague–Dawley rats used in renal transplantation
experiments were purchased from Harlan Laboratories. FVB/NJ mice were purchased from
The Jackson Laboratory.
All procedures using mice and rats were done in accordance with protocols approved by the
Lankenau IACUC.
Cell sorting and analysis
Prostate cells were suspended in PCT prostate epithelium medium Cn-T52 (Millipore). Cell
sorting was performed using a BD FACS AriaII cell sorter (BD Biosciences). Viable cells
were identified by trypan blue exclusion.
Peng et al.Page 3
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Prostate colony-forming assay
1 × 105 irradiated Swiss 3T3 fibroblast cells were plated onto vitrogen-fibronectin
(Cohesion Technology and Sigma) coated 24-well plates and cultured in DMEM/F12
medium (Gibco) supplemented with 10% fetal bovine serum (FBS) overnight. Two hundred
to 1,000 sorted cells/well were seeded onto the 3T3 feeder layer and cultured in William's
medium E (Invitrogen) plus supplement (Morris et al. 1990). After 7 days, colonies were
viewed using a microscope and counted.
Prostate sphere-forming assay
Sorted cells were counted and mixed in 100 ml 1:1 Matrigel (BD Bioscience) and CnT-52
medium (Millipore). The mixture was placed into the center of a 35 mm petri dish (Falcon)
5–10 min until the mixture solidified, after which 3 ml CnT-52 medium was added. The
cells were incubated at 37°C in 5% CO2, balance air. After 7 days, spheres were counted.
Recombinant cell renal grafts
Urogenital sinus mesenchyme (UGM) cells used in tissue regeneration were obtained from
day 18 pregnant SD rats. Urogenital sinuses (UGS) were collect from E18 embryos and
digested in 1% trypsin at 4°C for 70 min. UGM were separated from UGS epithelia (UGE).
UGM from 2 embryos were mixed with sorted prostate epithelial cells and resuspended in
20 ml of collagen Type I (BD Biosciences), which was pre-treated with NaOH in PBS
according to the manufacturer's protocol. Upon gelation at room temperature, 3 ml William's
medium was added. The cell mix was incubated overnight at 37°C in 5% CO2, balance air.
The next day, the cells were grafted beneath the kidney capsule of male athymic nude mice.
After 8 weeks, mice were euthanized and the grafts were photographed in situ using a Nikon
SMZ 1500 dissecting microscope with a Nikon digital camera DXM 1200F. They were then
excised and processed for immunohistochemical analysis.
Castration experiment
Three-month old K5/CFP-K18/RFP double transgenic mice were castrated. Two weeks
later, some mice were euthanized, their prostates were removed, and frozen sections
prepared. Other mice received daily subcutaneous injections of testosterone [60 mg in 100
ml peanut oil (Sigma)] for 2 weeks, after which they were euthanized and frozen sections of
their prostates were prepared. Sections were viewed under fluorescence using a Zeiss
Axiovert 200 M microscope and images were taken with an Axiocam digital camera.
Immunohistochemistry and immunocytochemistry
Sorted cells were fixed for 10 min in 2% paraformaldehyde, washed with 1.5% bovine
serum albumin (BSA) in phosphate buffered saline (PBS), then smeared on glass slides and
air dried. Cells were permeabilized with 0.25% Triton-X100 in PBS for 10 min. Following
quenching of endogenous peroxidase, cells were incubated with primary antibody at 4°C
overnight, then with biotinylated secondary antibody for 30 min at room temperature,
followed by streptavidin-CY3 for 20 min at room temperature. Images were taken using a
Zeiss Axiovert 200 M microscope equipped with an Axiocam digital camera.
K5/CFP prostate tissue and recombinant cell grafts were fixed in formalin (Fisher) for 2 h
and processed for paraffin embedding. Four micrometer sections were deparaffinized, and
antigen retrieval was performed by steam heating 30 min followed by endogenous
peroxidase quenching with 3% H2O2/methanol for 20 min. K18/RFP prostate tissue was
fixed in 4% paraformaldehyde (Electron Microscopy Science) for 5 min and then embedded
in O.C.T. compound (Tissue-Tek). Four micrometer sections were treated with 0.1% pepsin
in 0.01 N HCl at room temperature for 5 min, followed by endogenous peroxidase
Peng et al. Page 4
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quenching with 0.3% H2O2/PBS for 10 min. Both paraffin and frozen sections were
incubated with primary antibody at 4°C overnight, biotinylated secondary antibody for 30
min at room temperature, and streptavidin-HRP for 20 min at room temperature. Signals
were amplified and visualized using the TSA-Plus Fluorescence System (Perkin Elmer)
according to manufacturer's instructions. Images were taken using a Zeiss Axiovert 200 M
microscope or a Zeiss LSM510 META confocal fluorescent microscope.
The antibodies used in immunostaining were as follows: K5 (Covance), K8 (Covance), CFP
(Clonetech, EGFP antibody), AR (C-19) (sc-815, Santa Cruz), synaptophysin (clone sy38,
Millipore), and CD49f (BD Biosciences).
Total RNA from sorted CFP+ and CFP− epithelial cells was prepared using a PicoPure RNA
Isolation Kit (Arcturus) according to the manufacturer's protocol. Samples were sent to
Phalanx Biotech Group (CA) for amplification and OneArray™ Microarray service,
including single color labeling, three replications, hybridizations, scanning, signal
extractions, and basic data analysis. The mouse OneArray™ consists of 29,922 mouse
genome probes and 1,880 experimental control probes (see http://ww.phalanxbiotech.com
for more information. Genes that were differentially expressed between the CFP+ and CFP−
cell populations were evaluated for enriched biological pathways and gene ontology terms
using Ingenuity Pathway Analysis and the Database for Annotation, Visualization, and
Integrated Discovery (DAVID) at http://david.abcc.ncifcrf.gov. DAVID was also used to
perform functional clustering which organizes genes into groups containing similar
functional descriptions. Common themes among the highlighted functional categories are
growth and proliferation, DNA replication, cell signaling and adhesion, and cancer
Single cell one-step RT-PCR
One-step RT-PCR of single cells was performed using the AmpliGrid system (Advalytix,
Beckman Coulter) according to the manufacturer's protocol. In brief, cells were FACS-
sorted directly onto an AmpliGrid slide, and then air dried. Following deposition of 0.5 μl of
cell extraction reaction mix on each cell, 5 μl of sealing solution was added to each sample.
The AmpliGrid slide was placed on the AmpliSpeed slide cycler and heated (5 min @75°C,
2 min @ 95°C). 1 μl of RT-PCR master mix, made from AmpliGrid Single Cell RT-PCR
Kit, was added to each sample through the sealing solution, and the amplification program
was run for 40 cycles using an annealing temperature of 64°C. PCR results were assessed on
FlashGels (Lonza). The primers used for K5 and K18 were: K5 5′
Statistical significance of differences in the ability of different cell populations to form
clones was determined using the unpaired two-tailed student t test.
Transgenic model validation
We observed CFP and RFP fluorescence in the different lobes of the adult prostate and in
the developing prostate of K5/CFP transgenic mice and of K5/CFP-K18/RFP double
transgenic mice (Fig. 1). Fluorescent cells were localized to the epithelium of the adult
prostate and to the urogenital epithelium lining the urogenital sinus in fetuses at day 16 and
Peng et al.Page 5
Transgenic Res. Author manuscript; available in PMC 2012 October 1.
NIH-PA Author Manuscript
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NIH-PA Author Manuscript


text:
The House Intelligence Committee approved legislation Nov. 21 that would set policy and funding levels for intelligence community programs for fiscal 2014, including efforts to modernize IT and target insider threats.
The measure would authorize full funding for a Director of National Intelligence initiative to modernize and consolidate technology, although it also includes reductions in other areas of IT.
The bill would authorize $75 million to target insider threats in the wake of the Edward Snowden leaks that have embroiled the National Security Agency in a spying scandal this year.
Other provisions in the bill, which was approved by voice vote, would:
An amendment from Rep. Devin Nunes (R-Calif.) included in the bill would order a declassification review of documents seized from Osama bin Laden's compound in Abbottabad, Pakistan, after the raid that killed the al-Qaeda leader.
Another amendment, by Rep. Jim Langevin (D-R.I.), would expand opportunities for cybersecurity education.
"I am pleased that this committee continues to build upon the good, bipartisan work we began in 2011," Chairman Mike Rogers (R-Mich.) said in a released statement.
About the Author
Amber Corrin is a former staff writer for FCW and Defense Systems.
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In the newest Federal Employee Viewpoint Survey, feds show they're feeling better than they have over the past few years – but there's still room for improvement, especially in mission-critical IT.
The General Services Administration's Enterprise Infrastructure Solutions contract will anchor the agency's NS 2020 telecommunications strategy.
House and Senate both approve a measure to maintain funding at fiscal 2015 levels through Dec. 11.
The aviation regulator missed a Congressional deadline to develop rules of the sky-ways for drones.
Agency heads, acquisition officers and even CIOs don't need to be hard-core techies. FCW's four-part look at the expertise that's really required to make federal IT run.
FCW recognizes 14 individuals having an outsized impact early in their careers.
As Steve Kelman returns from a summer of aggressive medical treatment, he considers the all-too-common aversion to short-term sacrifices.
Massive open online courses can offer specialized IT instruction on a flexible schedule and on the cheap. That may not always mesh with government's preference for structure and certification, however.
The federal government craves more cybersecurity professionals. These six schools are helping meet that demand.
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text:
In 2015, the Faculty of Social Sciences (FSS) celebrates its 60th anniversary! Keep an eye out for articles and activities meant to highlight, throughout the year, our commitment to research, teaching and engagement.
The FSS comprises nine departments, schools and institutes, which offer undergraduate, masters and doctoral programs in both English and French. With its 10,000 students, 260 full-time professors, and wide array of programs and research centres, the Faculty of Social Sciences plays a key role at the heart of the University of Ottawa. Its graduate students are supervised by excellent researchers and undertake cutting-edge research in the Faculty's masters and Ph.D. programs.
- Oct. 8: Les classiques des sciences sociales Friedrich Nietzsche, Généalogie de la morale (2), Gilles Labelle (event in French only)
- Oct. 9: The United Nations at 70: Looking Ahead, Bob Rae, Louise Fréchette, School of Public and International Affairs, University of Ottawa, David Petrasek, graduate School of Public and International Affairs
- Oct. 13: Conventions, Confidence and Caretakers: Post-Election Possibilities, Peter Milliken and Philippe Lagassé
- Oct. 13: Europe on the Brink? Challenges of European Integration Today, Wolfgang Muno, Johannes Gutenberg University Mainz
- Oct. 15: Notes from the Archives: Preliminary Reflections on EH Carr, RG Collingwood and the Idea of History' as applied to IPE, Randall Germain and Dana Galizia, Carleton University


text:
How do I ensure the callback is called within the context of the main thread?
I'm using ActiveMQ and specifically, I'm trying to understand how to ensure a callback is executed within the context of the thread that created the activemq consumer.
The thread ID I get in the listener/handler is different from the one that created the consumer and set the listener, so I'm trying to understand how I can ensure my code in the handler/listener runs on the first thread thread.
If I had created the thread which runs the code in the listener, I probably would have used Executor and Future, but since it was created for me by activemq, I was hoping to figure out how to get the listener to execute within the context of the 'main' thread.
I then started reading about java threading callbacks in general, and most articles explain calling a method on a class you pass into the thread that implements runnable. It seems to me that any code in that callback will still be within the context of the thread I just spawned.
..so how do I ensure the callback is called within the context of the main thread?
In .net I could use the SynchronizationContext or in WPF the Dispatcher which uses this context under the hood..
I've been looking at synchronized methods but it looks like those methods access data variables defined in another thread, ie the 'main' thread where in my case, the variables/data are created in the consumer thread and I need to pass them back / ensure they are available on the 'main' thread.


text:
ClassicsOnline Home » BRITTEN: Folk Song Arrangements, Vol. 2 (English Song, Vol. 13) > Review List
Benjamin Britten was throughout his life drawn to folk-song, possessing a special gift for wordsetting and vocal writing. This second disc of the complete folk song arrangements focuses on the orchestral versions and the volumes for guitar and harp. This release and its companion (Naxos 8.557220-21), both previously available on Collins Classics, present all the known folksongs and include ten world première recordings of arrangements published as recently as 2001.
ClassicsOnline customers will not be able to use their log in to access the new HD site. Please create a new ClassicsOnline HD•LL account to download albums, subscribe to streaming, create playlists and more!


text:
"It's not whether you buy the car or don't buy the car," he said, but rather whether bankruptcy would put "unbearable stress" on the whole chain of auto manufacturing and auto dealers that rely on the car companies. "It would have a cataclysmic effect on the auto industry."
Mr. Nardelli described the additional loan he is requesting from the Treasury, which he would like to receive upon approval of his restructuring plan on March 31, as "bridge financing to get us through this economic trough."
Chrysler is predicting that U.S. auto sales will fall to a 40-year low of 10.1 million this year before picking up again in 2010 and 2011. It hopes to pay off its Treasury loans by 2012. GM presented a significantly brighter outlook, saying it expects sales to pick up to 11.5 million to 12 million in the next year, which would enable it to return to profitability within two years.
Despite weeks of negotiations with the United Auto Workers and bondholders, the companies said they were not able to obtain concessions in key areas targeted by the Treasury when it provided loans in January: reducing GM's $27.5 billion in public debt by two-thirds and securing agreement from the union to accept stock instead of cash contributions to their retiree health care funds.
Just before announcing the plans, however, the companies said they obtained concessions from the unions on some work rules as well as abandoning the job bank program, which provides supplemental unemployment benefits for laid-off workers.


text:
Taj Palace Hotel
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... room rate) at the hotel.
We've been to Delhi (population nearly 13 million) twice before (in 1977 and 1999) and have seen the main sights, so once again we're interested in seeing something a bit different. We've heard that Delhi has changed a lot recently, particularly because of the Commonwealth Games being held here last year, and we're interested to see if we agree. One of the new things ...
Shopping and Delhi Belly
... a break to have a lime soda mixed. After that, Brendan and I started having an upset tummy, and it was time to go, anyway. We decided that we wouldn't go to the Thursday night temple since it was getting late and we were tired. We made our way back to Beth's and immediately Brendan got sick. Uh oh. I wasn't feeling well either and got sick soon after. We spent the rest of the evening and night trying to get through ...
Full House
... I had been sent in a commision set-up. Darn.
When I got to the office, I asked for a train to Jaisalmer. The agent told me that the train was full. However, he offered another solution. "There's another man I can put you in touch with who will charge Rs2000 each way..." I kindly turned his sketchy offer down, but didn't escape without getting his business card. I swear- I will have a collection of business cards from ...


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Celebrating our Newly Refreshed, Renewed and Renovated PropertyAugust 11, 2015
The K Club is a 5 AA Red Star Hotel & Resort located in the pretty village of Straffan, Co. Kildare, Ireland, just 30 minutes from Dublin Airport and Dublin City Centre . The equivalent of an AAA Five Diamond property, The K Club is a proud, long-standing member of the Preferred Hotels & Resorts Legends Collection and is also a member of Virtuoso and American Express Fine Hotels & Resorts . Represented by Celebrated Experiences and Waterstone Marketing the resort is synonymous with luxury and elegance the world over.Read more... Category Hotel
The K Club Named in Top 5 Resorts in Europe, October 2014November 04, 2014
The 2014 Condé Nast Traveler Reader's Choice Awards has named The Kildare Hotel, Club & Spa in Kildare, Ireland as one of the Top Five Resorts in Europe. Appearing for the first time in the top five, this number four ranking is a true testament of The K Club's continued dedication to its guests and the luxury experience they deliver.Read more... Category Hotel
Christmas at Straffan House, October 2014October 20, 2014
A picture perfect holiday awaits at the luxurious K Club and its uber elegant Straffan House, a 34,000-sq-ft country home located on the hotel's grounds. The ultimate home away from home for the holidays, both offer rich plush interiors, numerous crackling fires, and gracious Christmas décor all surrounded by the lush Irish countryside.Read more... Category Hotel
Smashing Pumpkins, September 2014September 24, 2014
It's Treat Time at The K Club!
With the kids now well settled back to school after the summer holidays it's time to start planning your next 'great escape'. The Halloween midterm is always a great time to recharge the batteries and spend some valuable time with your little ghouls and goblins!Read more... Category Hotel
The K Club is to host a PGA Pro AmSeptember 24, 2014
The K Club are delighted to be supporting the Irish golf and the Irish Professional Golfers Association by hosting The K Club Irish PGA Pro Am over 2 days in May this year. The Pro-Am is being held on Monday 26th & Tuesday 27th and each team will play both the Ryder Cup Palmer Course and the Smurfit Course over the 2 days. The professional prize will be competitively fought for with a substantial prize fund on offer for the professionals. More information from 01-6017300 or firstname.lastname@example.com more... Category Golf
The K Club won two awards at The Restaurant Association of Ireland Awards NightSeptember 24, 2014
The K Club Wins Two Awards at Irish Restaurant AwardsRead more... Category Hotel
The K Thai at The K Club, May 2014September 24, 2014
The 5 AA Red Star, K Club has just launched K Thai, a brand new Thai & South East Asian restaurant situated in The Smurfit Clubhouse. The restaurant blends the very finest of Irish produce with spices and flavours of the east. Decorated in cool creams and antique whites, and overlooking the dramatic landscape of the Smurfit Golf Course, the restaurant is light and airy and is the perfect blend of contemporary and Asian design.Read more... Category Hotel
Viva la France!, Friday 19th May 2011May 19, 2011
Congratulations to France on winning the bid to host The Ryder Cup 2018!
After a highly competitive pitch to host the Ryder Cup 2018, France has been declared as the host country to hold the prestigious Ryder Cup matches. The 42nd edition of the matches will take place at Le Golf National which is located on the outskirts of Versailles, near Paris.Read more... Category Golf, Category Hotel
The K Club, featured on Escape SeekerMay 20, 2010
ESCAPESEEKER with Lynn Mc Cool & Mel Gee Henderson
TV programme · 1622 Likes · 20th May 2010Read more... Category Hotel
Evening Hearld, Friday 26th March 2010March 26, 2010
KILDARE: The K Club has organised a dedicated K Cubs' children's activity club with face painting, parties, a duck race on the Liffey, a fishing demonstration with the resident ghillie and an Easter egghunt.Read more... Category Hotel
Irish Independent, Saturday February 13 2010February 13, 2010
HE has had the rare distinction of having played characters that have been eaten by both a shark and a dinosaur.But yesterday the only thing facing down Samuel L Jackson was a birdie.Read more... Category Golf, Category Hotel


text: NJ State Senator Robert Singer (Townsquare Media) [ Shore-Based Bill Aims To Stop Student Athlete Cardiac Deaths By Tom Mongelli June 18, 2012 A shore-based state measure that would try to prevent cardiac-related student athlete deaths by requiring defibrillators at all school sports events inches forward... Read More|Comment Category: Briefs, Ocean County News Tags: Janet Zilinski, Janet's Law, Lakewood, Ocean County, WOBMFM Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


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Boys soccer: Cyril's blind goal gives Central the region semifinal win | Photo gallery
FORT PIERCE — Enly Cyril said he never saw the ball go in the net.
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Fire Eaters at Beach Party in St. Lucia [VIDEO]
Fire Eaters at Beach Party at Sandals in St. Lucia with Mimi
Fire Eaters at Beach Party at Sandals in St. Lucia with Mimi
Oh, how I love Sandals Resorts! I've been to quite a few all-inclusive resorts and Sandals is miles and miles above the rest!
Here are more photos from my amazing trip to Sandals Grande St. Lucian Spa & Beach Resort. Such a fabulous trip! They treated us like VIPs as soon as we stepped off the plane. Everyone was always smiling and so good to us...I am ready to go back!
I would love for you to win a FREE four day / three night Luxury Included® Vacation to the Sandals Resort of your choice with locations in Jamaica, Antigua, St. Lucia, Bahamas, Grenada and Barbados with airfare for two on American Airlines.
Make sure you are in the Rewards Club now and be sure to share the club with all of your friends. A lucky listener of ours is going to win this trip--don't you want it to be you?!
Make the most of this St. Patrick's Day this year by putting your smartphone to use with these St. Patrick's Day apps:
It's time to dust off the crystal ball. This year, 63 intense college basketball games will fill up the college basketball championship tournament bracket – it's your job to figure out who wins each one.
Katy Perry is back, and her Prismatic World Tour Kicks off this summer and we have you chance to see her live October 8th in New Orleans.
Enter to win a Disney On Ice party for your family and friends this May in Bossier City, La.
For the first time in its history, "Gala of the Royal Horses" is coming to North America. The world-renowned equestrian tour is slated to begin its upcoming series of events this Friday, February 7 in Kissimmee, Florida. The event will be at the Four States Fair Grounds on March 2.
"What Do You Think Wednesday" is your opportunity to say what is on your mind and you have a chance to win lunch for two from Three Chicks Cafe (formerly known as The Chuck Wagon).
Our question: What do you think about the flu shot?
Spending more than you want to this Christmas? A little help could go a long way paying for the kids' toys, laptops and games. Not to mention that special piece of jewelry for the wife, or the exciting hunting gear for the hubby. Enter now and you could win $3,000 to help pay off your holiday debt!
"What Do You Think Wednesday" is your opportunity to say what is on your mind and you have a chance to win lunch for two at the Chuck Wagon!
Our question: What is your most favorite Christmas movie ever?


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About Oakridge Custom Finishing, LLC
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Road * Suite 100 * Warner Robins, GA 31088
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OK…most folks call it "dipping", because that is exactly what it is. But, there is one giant difference for getting your work done at Oakridge. We are FACTORY CERTIFIED by TWN Industries. As a matter of fact, we travelled to Miami, Florida for our certification classes. We are not giving tours and we are not giving away our application and finishing secrets….you can go to Florida and spend a bunch of money for that….but we are ready for you to start looking to see what patterns you want on your firearms, switch plates, toilet seats (only new ones please), helmets, car wheels, etc.
As a reminder…we are also certified by Lauer for DuraCoat and by NIC Industries for Cerakote.
This probably makes Oakridge Custom Finishing one of the very few finishers holding three certifications in Georgia…probably in the Southeast!!
Come see us when you get a chance to discuss your color choice or camo choice needs.
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text:
Saw this report thought I would share. Please keep it bumping to keep everyone informed.
Saw this report thought I would share. Please keep it bumping to keep everyone informed.
I read about this and this is my opinion, but the babies who died were old enough to roll over and such, which got them trapped. We have one that we used with DS, and took it out once he was a little more mobile. These are made for infants, and infants don't roll around. Just take them out when they're starting to roll.
*Me (32)* Married to my best friend DH (33), SAHM to DS#1 (June 06), DS#2 (Feb.11), and ^10^ angels ~ waiting on God's timing.
If you read the article that was posted it was babies as young as one month dying. I don't see the need for those positioners. We used to use receiving blankets with dd but never anywhere near her face.
It was fun while it lasted. Se la ve BBC
I just saw something about this on the Today show this morning. They were talking about the Fischer Price recalls and then mentioned that the FDA does not recommend sleep positioners due to suffocation.
I could have sworn that the sleep positioners were not a good idea back when I was pregnant with my DS. I remember reading it somewhere that they weren't recommended.
Why is it just now that they're being recalled, when I knew in 2007 that they weren't safe?
Kappy ? Bradley est. 11/06
WARNING: �I am fluent in smart assery and sarcasm.
I'm attending�BabyCenter's Big Day Out!� Join us!!!
I sent the FDA report to my SIL...she is registered for one. I am pretty sure that will piss her off...oh well I alreayd like her baby more than her!
Mom to Teddy 1-12-09 and Liam 2-9-11 ( . ) ( . )
we used recieving blankets with my dd but i do have two of the sleep postioners but when we did use them she was only in it from her belly button down so it was no where near her face
proud wife to a solider
Jayden 9-1-08
I find it interesting that the article says to always place infants on their back for sleeping...when I gave birth to DD, they told us that it's safest to keep her on her side, so that if she refluxes in her sleep, you don't risk the chance of aspiration. We always just used receiving blankets to keep her rolled to her side slightly. Good thing we did, too, because that kid spat up like you wouldn't believe.
Strangers will often approach you to ask, "Is she a good baby?" Surprise them by saying, "No, she's kind of an asshole."
bombsquad505: When I was a baby they told my mom the best place for a sleeping baby was thier belly. The "rules" change so much it makes us moms a nervous wreck!!!
Oh my, I'm so stressed now! As a FTM I want to do what is best for my baby but now I'm so confused. Like PP said, I thought a baby on his/her back was bad because of reflux and choaking to death but now I'm reading that they need to be on their back to reduce the risk of SIDS!?!? Holy moly, I'm going to be a wreck with worry when my baby sleeps!
FTWM to BCS - 2/21/11 * 9lbs, 21.5"
"I'm only one person, but I can feel it working. I believe in better days. That's why I Pray." Big & Rich
I have a friend who did a lot of research on SIDs and made a choice for her baby that is not very popular with mainstream, etc. I don't know details since I haven't done the research myself, but basically there is a camp that believes that SIDs related deaths could be linked to the mattresses. When companies started putting fire retardants and other chemicals in the mattresses and babies faces and drool were right up in it that they would off-gas, etc. I'm sure there is more to it than that, but I haven't done the research for myself yet. My friend got an organic mattress and let her baby decide if it was more comfortable to sleep on tummy or back and baby picked tummy. She has had no problems and is very confident with her decision. I know this goes against what were are currently told though...
My sister told me to register for this thing that you can put the baby ont he bed and has two side pieces and a protective head area...I'm now wondering if that is considered a sleep positioner or not... :( Thinking of taking it off the registry.


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I'm very pleased to show a preview of Sue and Eric's wedding photos. Sue got ready at the Park Ridge Marriott in Park Ridge, NJ. The ceremony took place at St. Anthony's Church in Nanuet, NY. And the reception was held at Seasons in Washington Township, NJ.
Categories: WeddingsTags: bergen county wedding photographer, marriott, nanuet, park ridge, Seasons, Seasons Wedding, Seasons wedding photographer, Seasons wedding photos, st. anthony, Washington Township, Wedding at Seasons