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Today as we commemorate the life and legacy of Dr. Martin Luther King, Jr., it's easy to forget how despised King was in his own time by many on the right and the left, by many within the church and outside it. As the frequency of his public speeches increased toward the end of his life so did his visible rage; as his preaching evolved in the last years, he moved from what Richard Lischer has called a "homiletics of identification" to a "homiletics of confrontation." The radical politics of the Kingdom that King envisioned—for the church and the nation—did not endear him to either; it got him killed. Read more
It's hard to be cynical today.
It may be easier tomorrow, next week, or next month—it almost certainly will be. But today is a day for head-shaking wonder at what transpired on Nov. 4.
Even though it wasn't a surprise, the election of Barack Obama is epic for all the reasons the pundits have waxed eloquent about during the last twenty-four hours, and the margin of his victory is ample evidence that Senator McCain didn't lose the election: Senator Obama won it, and decisively. Read more
Why Share?
You may remember the Garrison Keillor story of why shopping at Ralph's Pretty Good Grocery in Lake Wobegon is preferable to shopping in St. Cloud at the new Higgledy-Piggledy. Ralph's Pretty Good Grocery has just gotten in a case of fresh cod. "Frozen, but it's fresher than what's been in his freezer for months. In the grocery business, you have to throw out stuff sometimes, but Ralph is Norwegian and it goes against his principles." On the other hand, more and more people have been "sneaking off to the Higgledy-Piggledy in St. Cloud, where you find two acres of food, a meat counter a block long with huge walloping roasts and steaks big enough to choke a cow, and exotic fish lying on crushed ice."
Keillor goes on to explain that Lake Wobegon does not run on free enterprise, which is based upon self-interest. It is run on loyalty. He goes on to say you can shop at the St. Cloud Mall instead of Lake Wobegon but the St. Cloud Mall isn't going to come with the Rescue Squad and they aren't going to teach your children redemption by grace. Read more
Choosing the Evil of Two Lessers
Months ago, at the beginning of the presidential rutting season, I reflected here on the comment of a Jewish friend of mine, who said he never felt more alien in the United States than at Christmas. I'm nearly with him on that, seeing how far the consumer capitalist Winter Holiday runs from the appalling mystery of the Incarnation. Yet it's hard to blame this culture and economy from avoiding that unprofitable Jesus business which, in the words of the late great British sitcom, Blackadder, "always spoils the Xmas atmos." We may still call it Christ-mas, but Yuletide in America makes us all anonymous pagans. Read more
Immigration and the Crumbs from Our Table
"You speak of signs and wonders / I need something other / I would believe if I was able / But I'm waiting on the crumbs from your table. ("Crumbs From Your Table," U2, How to Dismantle an Atomic Bomb)
It's become something of a tradition: I start a conversation via email with a large distribution list I have, made up mostly of fellow church members but also including some far-flung friends and colleagues. Often, I share my bLOGOS reflections on the lectionary or make a plea for help with a project or program; sometimes I simply direct folks to an interesting website or blog. The point is not to court controversy for its own sake, but sometimes the topics and the ensuing conversation take us into complex social, political, or theological issues where the moral murkiness can be difficult to navigate. Read more


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Thread: Martial Arts Boarding School
7/22/2010 12:32am, #1
- Join Date
- Jul 2010
- Location
- Tampa, Fl
- Posts
- 1
Martial Arts Boarding School
I was thinking of opening a Martial Arts boarding school and i'm looking for people interested. It would be a school teaching tradition martial arts, and dedicated to preserving the history and credibility of martial arts.
Last edited by Arcon3; 7/22/2010 12:32am at . Reason: typo
7/26/2010 9:49am, #2
- Join Date
- Jul 2010
- Posts
- 5
i would go, what age group would it be? if i cant go, maybe my kids will :p
7/26/2010 10:01am, #3
7/26/2010 11:28am, #4
- Join Date
- Apr 2010
- Location
- West Virginia
- Posts
- 1,945
What kind of martial arts?
Will you be teaching? What are your qualifications?
What are the qualifications of other teachers if there are any?
Do you have a facility and a list of dedicated students from the area already?
Do you have any degrees in history or have any peer-reviewed research into historical matters relating to martial arts?
What fees will be involved? Will room and board be provided in sanitary conditions?
Do you have the proper permits and insurances as given by your local government?
I could go on all day.
7/26/2010 4:01pm, #5--
this sounds like the premise to an anime series.
7/26/2010 6:51pm, #6
3/11/2011 8:46am, #7
3/11/2011 10:49am, #8--
3/11/2011 12:34pm, #9--Writers: Yuk Long Wong (comic series Dragon and the Tiger Heroes), Edmond Wong (screenplay)Fight Film Friday
Watching violence on film, violently.
Click here to donate!
3/11/2011 12:40pm, #10

subset: commoncrawl


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it's almost a month till christmas. and if your supermarkets are anything like ours, you've been bombarded with christmas music since october. if only they'd play this holiday album from target, filled with tunes from rad bands like wavves, best coast and coconut records amongst others. you'll be able to download the songs for free on 28 november.
nom nom nom
just had to show you the way my sister-in-law wrapped all the homemade stollen & cookies that she gave everyone at christmas (cut-out reindeer tags!):
ho-ho-ho-holy crap it's christmas already!
(that's me, obviously happy with my christmas loot)
i hope you have a lovely day, whether you're in the snow or the sun… my christmas is going to consist of opening gifts sometime late morning (or rather, watching as my young nieces and nephews tear open their presents, and then ask if they can open mine too).
kris·miss·moss gift guide
i'm thinking about christmas already. sigh. my family has instituted a one gift per person R100 limit (to combat the "meltdown"). i told them R100 is almost impossible, so i'm shooting for under 200 bucks. anything handmade is great to me, and anything i can order online is even better. if i can walk to a shop – wonderful, but that kind of limits you geographically & since i'm out in "the sticks" (as some might say) i welcome e-commerce with open arms (i'm also lazy). PLUS i'd also like to inject some christmas cheer into the south african postal service (i have faith!) – herewith my south african friendly online shopping gift guide


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Obama's latest economic push has familiar feel - 0 Comments
WASHINGTON — If President Barack Obama's new focus on the economy sounds familiar, that's because he's done it before.
Since the first year of his presidency, Obama has been launching — and re-launching — initiatives on the economy. Some came with new policy proposals, others with catchy slogans.
Remember 2011's "Winning the Future" campaign? Or the "We Can't Wait" initiatives that followed later that year? Just a few months ago, Obama was headlining the "Middle Class Jobs and Opportunity Tour."


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Neoseeker : Games : Xbox 360 : Rhythm & Music : DanceMasters
cheats | faqs | screens | forums | xboxlive hub | gamertags | leaderboard
BackgroundThe next generation in full body dancing is finally here! DanceMasters utilizes Microsoft Natal to give gamers the ultimate dance club right in their living rooms, with no peripherals required!
- Dance freely, no peripherals to tie you down!
- Choose from different modes to give yourself the ultimate Dance experience:
- Dance Mode
- Play your favorite songs
- Unlock the songs by clearing the music set lists
- Play simultaneously with 2 players
- Claim the title for Dance Master
- Lesson Mode (Tutorial)
- Status Mode
- Earn and collect titles
- Xbox Live
- Communicate with other Dancers around the world!
- Expand your dance experience with Downloadable Content
- Additional Dance Sequences
- Theme Packs
- Icon Pack
- Challenge yourself and your friends multiple difficulty levels:
- 1 or 2 markers per 1 bar
- STEP RIPPLES and POSING, and occasional STREAMS
- 1 or 2 marker per 1 bar
- Basically STEP RIPPLES and POSING, with moderate NORMAL RIPPLES, FRONT RIPPLES, ROCK RIPPLES and STREAMS
- 2 or more markers per bar
- Markers in eighth will appear
- Entire variation of the RIPPLES will appear
- Hardest of all!
- EXTREME mode with the markers OFF screen
- Just follow the MODEL's movement!
- Dance yourself to the music that will make your move with a wide variety of dance music including Hip Hop, R&B, Pop and Techno
- 30 Tracks
- Play NON-STOP set lists assorted by the Genre
- Keep up your moves, by monitoring how good you are dancing:
- Save your dance moves and watch them as a back-up dancer
- Take a snapshot doing your favorite dance pose and save to your local album and share with your friends!
- Watch yourself dance on screen, and learn how to improve your moves
- Challenge up to 4 of your friends online to the ultimate Dance Off
Intoxicationadded a cheat
Nov 06, 10 11:43am
Kinect launch line-up features lots of motion-intensive titles
With Kinect's launch just a few weeks away, it's a good idea to review what's on tap for anyone interested in... posted Oct 19, 10 7:19pm
Konami opens Tokyo Game Show website with Castlevania and Metal Gear
[image2 width=650 height=200] With the 2010 Tokyo Game Show approaching in a few weeks, Japanese game... posted Sep 01, 10 6:05am
Krunaladded 3 new screenshots
Jun 16, 10 12:05am
- Genre: Rhythm & Music (Xbox 360)
- Developer: Konami
- Publisher: Konami
Released North America Nov 4, 2010 Europe Nov 10, 2010 Japan Nov 20, 2010 Australia Nov 18, 2010
- Also known as:
- DanceEvolution in Japan / Europe / Australia


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10 Steps to Pick the Right Doctor
Whether you need a primary care doctor or a specialist, these 10 steps will ensure you find the right doctor for you. View Slideshow
Dr. Brady's Experience
- Psychiatry
Board Certifications
- Psychiatry
Procedures Dr. Brady Performs
Dr. Brady does not have any procedures listed. If you are Dr. Brady and would like to add procedures you perform, please update your free profile.
- Adjustment Disorder
- Attention Deficit Hyperactivity Disorder (ADHD)
- Autism
- Bipolar Disorder
- Developmental and Learning Disorders
- Major Depressive Disorder
- Post-Traumatic Stress Disorder (PTSD)
- Psychosis Due to Mental Illness
- Schizophrenia
- Schizophreniform and Schizoaffective Disorder
- Sexual Disorders and Gender Identity Disorders
Background Check for Dr. Brady
Malpractice Claims not available
- Healthgrades does not collect malpractice information for Ohio.
- No sanctions history found for the years that Healthgrades collects data.
0 Board Actions
- No board actions found for the years that Healthgrades collects data.
Awards & Recognitions
Education & Training
Medical School
- University of Toledo
- Graduated in 1991
Residency Hospital
- Med College Of Ohio
- Mercy Hospital
Languages Spoken by Dr. Brady & Staff
- English


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|By Greg Schulz
Article Rating:
|December 25, 2009 03:00 AM EST
Cloud Expo New York
There is a ton of discussion, stories, articles, videos, conferences and blogs about the benefits and value proposition of cloud computing.
Not to mention, discussion or debates about what is or what is not a cloud or cloud product, service or architecture including some perspectives and polls from me.
[Santa Photo Via ABC news]
Now SANta does not really care about these and other similar debates I have learned.
However he is concerned with who has been naughty and nice as well watching out for impersonators or members of his crew who misbehave.
In the spirit of the holidays, how about a quick look at how SANta leverages cloud technologies to support his global operations.
Many in IT think that SANta bases his operations out of the North Pole as it is convenient for him to cool all of his servers, storage, networks and telecom equipment (which it is).
However its also centrally located (See chart) for the northern hemisphere (folks down under may get serviced via SANtas secret Antarctica base of operations).
Just like ANC (Anchorage International Airport) is a popular cargo transient, transload and refueling base for cargo carriers, SANta also leverages the north and South Pole regions to his advantage.
[Photo: SANtas Global Reach via Great Circle Mapper]
Now do not worry if you have never heard about SANta dual redundant South Pole operations, its one of his better kept secrets. Many organizations including SANtas partners such as Microsoft that have global mega IT operations and logistics centers have followed SANtas lead of leveraging various locations outside of the pacific northwest. Granted like some of his partners and managed service providers, he does maintain a presence in Washington Columbia river basin which provides a nice PR among other benefits.
Likewise, many in business as well as those in IT think that SANta leverages cloud technologies for cost savings or avoidance which is partially the case. However he also leverages cloud, hosting, managed service provider (MSP), virtual data centers, virtual operations centers, Xaas, SaaS or SOA technologies, services, protocols and products that are transparent and complimentary to his own in house resources addressing various business and service requirement needs.
What this has to do with the holidays and clouds is that you may not realize how Santa or St. Nick if you prefer (feel free to plug in whoever you like if Santa or St. Nick does not turn your crank) extensively relies on flexible and scalable resilient technologies for boosting productivity in a cost effective manner. Some of it is IT related, some of it is not. For example, from the GPS and Radar along with recently added RNP and RNAV enhanced capabilities to his increasingly high tech bio fueled powered sleigh, not to mention his information technology (IT) that powers his global operations, old St Nick has got it together when it comes to technology.
The heart or brains of the SANta operation is his global system operations center (SOC) or network operation center (NOC) that rivals those seen at NASA among others with multiple data feeds. The SOC is a 24x365 operations function that covers all aspects from transportation, logistics, distribution, assembly or packaging, financials back office, CRM, IT and communications among other functions.
SOCs and NOCs that enable SANta to get those SOCKs above fireplace filled!
Naturally, like the Apollo moon shots whose Grumman built LEM Lunar lander had to have 100% availability in that to get off of the moon, their engines only had to fire once, however it had to work 100% of the time! This thought process is said to have had leveraged principles from SANtas operations guide where he has one night a year to accomplish the impossible.
I should mention, while I cannot disclose (due to NDA) the exact locations of the SOCs, data or logistics centers, not to mention the vendors or the technology being used, I can tell you that they are all around you! The fully redundant SOCs, data and call centers as well as logistics sites (including staff, facilities, technology) leverage different time zones for efficiency.
SANtas staff have also found that the redundant SOCs, part of an approach across Santa entire vast organization has helped to guard against global epidemics and pandemics including SARs and H1N1 among others by isolating workers while providing appropriate coverage and availability, something many large organizations have since followed.
Carrying through on the philosophy of redundant SOCs, all other aspects of SANtas operations are distributed yet with centralized coordinated management, leveraging real-time situation awareness, event and activity correlation (what we used to call or refer to as AI), cross technology domain management, proactive monitoring and planning yet with ability for on the spot decision making.
What this means is that the various locations have ability to make localized decisions on the spot. However coordinated with primary operations or mission control to streamline global operations focus on strategic activity along with exceptions handling to be more effective. Thus it is not fully distributed nor fully centralized, rather a hybrid in terms of management, technologies and the way they work.
For example, to handle the diverse applications, there are some primary large processing and data retention facilities that backup, replicate information to other peer sites as well as smaller regional remote office branch offices close to where information services are needed. To say the environment is highly virtualized would be an understatement.
Likewise, optimization is key not just to keep costs low or avoid overheating some of SANtas facilities that are located in the Arctic and Antarctic regions that could melt the ice cap; they are also optimized to keep response time as low as possible while boosting productivity.
Thus, SANta has to rely on very robust and diverse communications networking leveraging LAN, SAN, MAN, WAN, POTS and PANs among other technologies. For example, his communications portfolio is said to involves landlines (copper and optical), RF including microwave and other radio based commutations supporting or using 3G, 4G, MPLS, SONET/SCH, xWDM, Microwave and Free space optics among others.
SANtas networking and communications elves are also said to be working with 5G and 100GbE multiplexed on 256 lambda WDM trunk circuits in non core trunk applications. Of course given the airborne operations, satellite and ACARS are a must to avoid over flying a destination while remaining in positive control during low visibility. Note that Santa routinely makes more CAT 3+ low visibility landings than most of the worlds airlines, air freight companies combined.
SANtas has GUI and Management Displays in his Sleigh
My sources also tell me that SANta has virtual desktop capability leveraging PCoIP and other optimizations on his primary and backup sleighs enabling rapid reconfiguration for changing workload conditions. He also is fully equipped with onboard social media capabilities for updates via twitter, Face book and Linked In among others designed by his chief social networking elf.
Consequently, given the vast amount of information needed to support his operations from CRM, shipping, tracking not to mention historical and profiling needs, transactional volumes both on the data as well as voice and social media networks dwarf the stock market trading volume.
Feeding SANtas vast organizations are online highly available robust databases for transactions purposes, reference unstructured data material including videos, websites and more. Some of which look hauntingly familiar given those that are part of SANtas eWorld Helpers initiative including: Sears, Amazon, NetFlix, Target, Albertsons, Staples, EMC, Wall mart, Overstock, RadioShack, Landsend, Dell, HP, eBay, Lowes, Publix, emusic, Riteaid and Supervalu among others (Im just sayin...).
The actual size of SANta information repository is a closely regarded secret as is the exact topology, schema and content structure. However it is understood that on peak days SANtas highly distributed high performance, low latency data warehouse sees upwards of 1,225PBytes of data added, one that is rumored to make Larry Ellison gush with excitement over its growth possibilities.
How does SANta pull this all off is by leveraging virtualization, automation, efficient and enabling technologies that allow him and elves (excuse me, associates or team members) to be more productivity in their areas of focus that is the envy of the universe.
Some of their efficiency is measured in terms of:
- How many packages can be processed per elf with minimum or no mistakes
- Number of calls, requests, inquiries per day per elf in a friendly and understandable manner
- Knowing who has been naughty or nice in the blink of an eye including historical profiles
- Virtual machines (VM) or physical machine (PM) servers managed per team member
- Databases and applications, local and remote, logical and physical per team member
- Storage in terms of PByte and Exabyte managed to given service level per team member
- Network circuits and bandwidth with fewest dropped packets (or packages) per member
- Fewest misdirected packages as well as aborted landings per crew
- Fewest pounds gained from consumption of most milk and cookies per crew
From how many packages can be processed per hour, to the number of virtual servers per person, PBytes of data managed per person, network connections and circuits per person, databases and applications per person to takes and landings (SANta has the top of the list for this one), they are all highly efficient and effective.
Likewise, SANta leverages the partners in his SANtas eWORLD Helpers initiative network to help out where of course he looks for value; however value is not just lowest price per VM, lowest cost per TByte or cost per bandwidth. For SANta it is also very focused on performance, availability, capacity and economic efficiency not to mention quality with an environmentally friendly green supply chain.
By having a green supply chain, SANta leverages from a responsible, global approach that also makes economic sense on where to manufacture and produce or procure products. Contrary to growing popular belief, locally produced may not always be the most environmentally as well as economically favorable approach. For example (read more here), instead of growing flowers and plans in western Europe where they are consumed, a process that would require more energy for heat, lights, not to mention water and other resources. SANta has bucked the trend instead relying on the economics and environmental benefit of leveraging flowers and plants grown in warmer, sunnier climates.
Granted and rest assured, SANta still has an army of elves busily putting things together in his own factories along with managing IT related activities in a economically positive manner.
SANta has also leveraged this thinking to his data and information and communications networks leveraging sites such as in the arctic where solar power can be used during summer months along with cooling economizers to offset the impact of batteries, workload is shifted around the world as needed. This approach is rumored to be the envy of the US EPA Energy Star for Server, Storage and Data Center crew not to mention their followers.
How does SANta make sure all of the data and information is protected and available? Its a combination of best practices, techniques, technologies including hardware, software, data protection management tools, disk, dedupe, compression, tape and cloud among others.
Rest assured, if it is in the technology buzzword bingo book, it is a good bet that it has been tested in one of SANtas facilities, or, partner sites long before you hear about it even under a strict NDA discussion with one of his elves (opps, I mean supplier partners).
When asked of the importance of his information and data networks, resources and cloud enabled highly virtualized efficient operations SANta responded with a simple:
Ho Ho Ho, Merry Christmas to all, and to all, a good night!
As you sit back and relax, reflect, recreate, recoup or recharge, or whatever it is that you do this time of the year, take a moment to think about and thank all of SANtas helpers. They are the ones that work behind the scenes in SANtas facilities as well as his partners or suppliers, some in the clouds, some on or underground to make the worlds largest single event day (excuse me, night) possible! Or, is this SANta and cloud thing all just one big fantasy?
Happy and safe holidays or whatever you want to refer to it as, best wishes and thanks!
BTW: FTC disclosure information can be found here!
Me on a break during tour SANta site tour
Cheers and seasons greetings gs
Greg Schulz - StorageIO, Author "The Green and Virtual Data Center" (CRC)
Electric power utilities face relentless pressure on their financial performance, and reducing distribution grid losses is one of the last untapped opportunities to meet their business goals. Combining IoT-enabled sensors and cloud-based data analytics, utilities now are able to find, quantify and reduce losses faster – and with a smaller IT footprint. Solutions exist using Internet-enabled sensors deployed temporarily at strategic locations within the distribution grid to measure actual line loads.
Oct. 10, 2015 02:00 PM EDT Reads: 151
The Internet of Everything is re-shaping technology trends–moving away from "request/response" architecture to an "always-on" Streaming Web where data is in constant motion and secure, reliable communication is an absolute necessity. As more and more THINGS go online, the challenges that developers will need to address will only increase exponentially. In his session at @ThingsExpo, Todd Greene, Founder & CEO of PubNub, will explore the current state of IoT connectivity and review key trends and technology requirements that will drive the Internet of Things from hype to reality.
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SYS-CON Events announced today that IBM Cloud Data Services has been named "Bronze Sponsor" of SYS-CON's 17th Cloud Expo, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. IBM Cloud Data Services offers a portfolio of integrated, best-of-breed cloud data services for developers focused on mobile computing and analytics use cases.
Oct. 10, 2015 01:00 PM EDT Reads: 756
The Internet of Things (IoT) is growing rapidly by extending current technologies, products and networks. By 2020, Cisco estimates there will be 50 billion connected devices. Gartner has forecast revenues of over $300 billion, just to IoT suppliers. Now is the time to figure out how you'll make money – not just create innovative products. With hundreds of new products and companies jumping into the IoT fray every month, there's no shortage of innovation. Despite this, McKinsey/VisionMobile data shows "less than 10 percent of IoT developers are making enough to support a reasonably sized team....
Oct. 10, 2015 01:00 PM EDT Reads: 269
Today air travel is a minefield of delays, hassles and customer disappointment. Airlines struggle to revitalize the experience. GE and M2Mi will demonstrate practical examples of how IoT solutions are helping airlines bring back personalization, reduce trip time and improve reliability. In their session at @ThingsExpo, Shyam Varan Nath, Principal Architect with GE, and Dr. Sarah Cooper, M2Mi's VP Business Development and Engineering, will explore the IoT cloud-based platform technologies driving this change including privacy controls, data transparency and integration of real time context w...
Oct. 10, 2015 01:00 PM EDT Reads: 164
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Oct. 10, 2015 11:00 AM EDT Reads: 135
Developing software for the Internet of Things (IoT) comes with its own set of challenges. Security, privacy, and unified standards are a few key issues. In addition, each IoT product is comprised of at least three separate application components: the software embedded in the device, the backend big-data service, and the mobile application for the end user's controls. Each component is developed by a different team, using different technologies and practices, and deployed to a different stack/target - this makes the integration of these separate pipelines and the coordination of software upd...
Oct. 10, 2015 11:00 AM EDT Reads: 336
As a company adopts a DevOps approach to software development, what are key things that both the Dev and Ops side of the business must keep in mind to ensure effective continuous delivery? In his session at DevOps Summit, Mark Hydar, Head of DevOps, Ericsson TV Platforms, will share best practices and provide helpful tips for Ops teams to adopt an open line of communication with the development side of the house to ensure success between the two sides.
Oct. 10, 2015 11:00 AM EDT Reads: 643
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Oct. 10, 2015 10:15 AM EDT Reads: 338
SYS-CON Events announced today that Sandy Carter, IBM General Manager Cloud Ecosystem and Developers, and a Social Business Evangelist, will keynote at the 17th International Cloud Expo®, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA.
Oct. 10, 2015 10:00 AM EDT Reads: 152
The IoT market is on track to hit $7.1 trillion in 2020. The reality is that only a handful of companies are ready for this massive demand. There are a lot of barriers, paint points, traps, and hidden roadblocks. How can we deal with these issues and challenges? The paradigm has changed. Old-style ad-hoc trial-and-error ways will certainly lead you to the dead end. What is mandatory is an overarching and adaptive approach to effectively handle the rapid changes and exponential growth.
Oct. 10, 2015 10:00 AM EDT Reads: 257
The IoT is upon us, but today's databases, built on 30-year-old math, require multiple platforms to create a single solution. Data demands of the IoT require Big Data systems that can handle ingest, transactions and analytics concurrently adapting to varied situations as they occur, with speed at scale. In his session at @ThingsExpo, Chad Jones, chief strategy officer at Deep Information Sciences, will look differently at IoT data so enterprises can fully leverage their IoT potential. He'll share tips on how to speed up business initiatives, harness Big Data and remain one step ahead by apply...
Oct. 10, 2015 09:00 AM EDT Reads: 653
WebRTC converts the entire network into a ubiquitous communications cloud thereby connecting anytime, anywhere through any point. In his session at WebRTC Summit,, Mark Castleman, EIR at Bell Labs and Head of Future X Labs, will discuss how the transformational nature of communications is achieved through the democratizing force of WebRTC. WebRTC is doing for voice what HTML did for web content.
Oct. 10, 2015 09:00 AM EDT Reads: 1,448
"Matrix is an ambitious open standard and implementation that's set up to break down the fragmentation problems that exist in IP messaging and VoIP communication," explained John Woolf, Technical Evangelist at Matrix, in this SYS-CON.tv interview at @ThingsExpo, held Nov 4–6, 2014, at the Santa Clara Convention Center in Santa Clara, CA.
Oct. 10, 2015 07:00 AM EDT Reads: 5,959
WebRTC has had a real tough three or four years, and so have those working with it. Only a few short years ago, the development world were excited about WebRTC and proclaiming how awesome it was. You might have played with the technology a couple of years ago, only to find the extra infrastructure requirements were painful to implement and poorly documented. This probably left a bitter taste in your mouth, especially when things went wrong.
Oct. 10, 2015 06:00 AM EDT Reads: 828
Nowadays, a large number of sensors and devices are connected to the network. Leading-edge IoT technologies integrate various types of sensor data to create a new value for several business decision scenarios. The transparent cloud is a model of a new IoT emergence service platform. Many service providers store and access various types of sensor data in order to create and find out new business values by integrating such data.
Oct. 10, 2015 04:00 AM EDT Reads: 611
The broad selection of hardware, the rapid evolution of operating systems and the time-to-market for mobile apps has been so rapid that new challenges for developers and engineers arise every day. Security, testing, hosting, and other metrics have to be considered through the process. In his session at Big Data Expo, Walter Maguire, Chief Field Technologist, HP Big Data Group, at Hewlett-Packard, will discuss the challenges faced by developers and a composite Big Data applications builder, focusing on how to help solve the problems that developers are continuously battling.
Oct. 10, 2015 04:00 AM EDT Reads: 523
There are so many tools and techniques for data analytics that even for a data scientist the choices, possible systems, and even the types of data can be daunting. In his session at @ThingsExpo, Chris Harrold, Global CTO for Big Data Solutions for EMC Corporation, will show how to perform a simple, but meaningful analysis of social sentiment data using freely available tools that take only minutes to download and install. Participants will get the download information, scripts, and complete end-to-end walkthrough of the analysis from start to finish. Participants will also be given the pract...
Oct. 10, 2015 03:00 AM EDT Reads: 343
WebRTC: together these advances have created a perfect storm of technologies that are disrupting and transforming classic communications models and ecosystems. In his session at WebRTC Summit, Cary Bran, VP of Innovation and New Ventures at Plantronics and PLT Labs, will provide an overview of this technological shift, including associated business and consumer communications impacts, and opportunities it may enable, complement or entirely transform.
Oct. 10, 2015 02:15 AM EDT Reads: 773
SYS-CON Events announced today that Dyn, the worldwide leader in Internet Performance, will exhibit at SYS-CON's 17th International Cloud Expo®, which will take place on November 3-5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. Dyn is a cloud-based Internet Performance company. Dyn helps companies monitor, control, and optimize online infrastructure for an exceptional end-user experience. Through a world-class network and unrivaled, objective intelligence into Internet conditions, Dyn ensures traffic gets delivered faster, safer, and more reliably than ever.
Oct. 10, 2015 02:00 AM EDT Reads: 665


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Many thanks to Pivot Josh Susser for his hard work co-coordinating this past weekend's Golden Gate Ruby Conference. We also want to thank our Live Blogging team: Chad, Ryan, Zach, and David — who all did an excellent job of documenting the event. We also recorded videos of every talk, which will be posted to Talks within a week or so.
Something to be aware of if you are running your production app on EngineYard: Because of their shared disk setup, running disk intensive jobs on your staging environment may affect the performance of your production environment on the same slice. One of our teams ran into severe slowness on production due to tasks running on staging. This problem isn't immediately apparent because of the way the EY storage system is well abstracted for each environment. Submit a ticket to EY support for more details if this is a problem for you.
It seems that EngineYard should be monitoring the performance of their dom0s and the device serving your disk up. They should have contacted you before you contacted them.


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LibDex - The Library Index - Ireland
A worldwide directory of links to library homepages and web-based OPACs (Online Public Access Catalogs).
Military Archives is the place of deposit for the records of the Department of Defence, the Defence Forces and the Army Pensions Board in Ireland. Apart from serving the evidential, legal and heritage needs of the Defence Forces and the Department of Defence, Military Archives can provide public services to a broad spectrum of interests, from family history queries to academic research, to material for television and radio documentaries. Located in Dublin, Ireland.
Company archive of the Lattice Group which holds the historical records of the UK gas industry dating from the early 19th Century.
National Museums Northern Ireland
Across four unique sites, we care for and present inspirational collections that reflect the creativity, innovation, history, culture and people of Northern Ireland.
Oireachtas Library & Research Service
Documents Laid and Historical Collections. Following Irish independence, the newly established Dáil Library in Dublin sought out materials for purchase, with a view to building up the Library's collections. These materials are now available to view or download, and the Library & Research Service will add other historical materials in the future.
Parliamentary Archives: House of Lords Record Office
The archive service for both Houses of Parliament and facilitates public access to several million records which are preserved in the Victoria Tower repository. The records include original Acts of Parliament from 1497, Journals from 1510, papers laid before Parliament from 1531, Peerage papers from 1597, Judicial papers from 1621, and plans of canals, roads, railways and other public works deposited in connection with private bills from 1794. Amongst the comparatively few records of genealogical interest are the Protestation returns of 1642 and certain Papist returns.
Lists of people entitled to vote, or of people who voted, at elections. A freeholder was a man who owned his land outright (in fee) or who held it by lease which could be for one or more lives (for example, his own life or for the lives of other people named in the lease). Search includes surname & forename as well as address and a scanned image of the printed list where the name appears.
Original volumes held by the Public Record Office of Northern Ireland (PRONI) covering the years 1819 to 1900.
Contains just under half a million original signatures and addresses of the men who, on 28 September 1912, signed the Ulster Covenant, and of the women who signed the parallel Declaration. In total, the Covenant was signed by 237,368 men, and the Declaration by 234,046 women. Search provides surname & forename as well as address and a scanned image of their signature.
The first phase of a project to index and digitise all the early wills that were proved in the District Probate Registries of Armagh, Belfast and Londonderry from 1858 to c.1900. View the entire will calendar entry for each successful search. The second phase will link the index entries to both the will calendar entries and the copy wills images.
RASCAL (Research And Special Collections Available Locally)
Searchable online directory of collections available in Northern Ireland of significant benefit to researchers in the Humanities and Social Sciences.
Repositories of Primary Sources - Europe - United Kingdom
A list of links to online resources from the Univ. of Idaho Library, Special Collections and Archives.
Repositories of Primary Sources - Europe - Ireland
A list of links to online resources from the Univ. of Idaho Library, Special Collections and Archives.
Representative Church Body Library
The Library is the Church of Ireland's principal repository for its archives & manuscripts. Records from parishes, dioceses & cathedrals in the Republic of Ireland & the non-current records of the General Synod & the Representative Church Body have been transferred to the Library, although some records remain in local custody.
Survey of Jewish Archives in the UK and Ireland
From the University of Southampton Libraries.
A portal which offers access to the combined resources (books, magazines, journals.... - both digital and non-digital) of the 45 national libraries of Europe. It offers free searching and delivers digital objects - some free, some for a fee.
Kew, Richmond, Surrey, United Kingdom.
1901 Census of England and Wales Online
Pay-for-use online searchable index with online images available.
Searching the index is free. There is a small fee to download a digital image of a document.
Descriptions of more than 10 million records held by The National Archives of England, Wales and the United Kingdom. The visitor may either search or browse through the descriptions. Images of the documents are not available. Visitors can request copies online and can also place advance orders for the day of their visit.
This is an alphabetical list of the topical Research Guides. The guides help you find what you need in the catalogue, so this should be the first thing you consult.
United Kingdom & Ireland » Ireland & Northern Ireland » Libraries, Archives & Museums
106 Links


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Page 1
Crystal Structures of the Luciferase and Green Fluorescent
Protein from Renilla reniformis
Andreas Markus Loening1,2,3, Timothy David Fenn1,4, and Sanjiv Sam Gambhira,*,2,3
2 Molecular Imaging Program at Stanford, Department of Radiology and Bio-X Program, Stanford
University, Stanford, California
3 Department of Bioengineering, Stanford University, Stanford, California
4 Department of Molecular and Cellular Physiology, Stanford University, Stanford, California
Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in
molecular biology as a reporter gene in both cell culture experiments and small animal imaging. To
accomplish this bioluminesce, the 37 KDa enzyme catalyzes the degradation of its substrate
coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon
dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this
important protein (RLuc8), and present the first structures for any coelenterazine-using luciferase.
These structures are based on high resolution data measured to 1.4 Å and demonstrate a classic α/β-
hydrolase fold. We also present data of a coe-lenteramide bound-luciferase, and reason that this
structure represents a secondary conformational form following shift of the product out of the primary
active site. During the course of this work, the structure of the luciferase's accessory green fluorescent
protein (RrGFP) was determined as well and shown to be highly similar to that of Aequorea GFP.
luciferase; green fluorescent protein; Renilla reniformis; coelenterazine; alpha/beta-hydrolase
Luciferases have become important research tools over the last two decades, due to their ability
to emit light and therefore be monitored externally to the milieu they reside in. This ability has
seen these bioluminescent proteins utilized widely as reporter genes in cell culture experiments
and more recently in the context of small animal imaging [1]. The two main classes of
luciferases employed as research tools are the beetle and coelenterazine luciferases. The beetle
luciferases (e. g. firefly) use D-luciferin as their substrate, are highly similar (≥ 45% [2]), and
have been extensively studied including structurally [3].
Corresponding author: Email address: E-mail: email@example.com (Sanjiv Sam Gambhir).
1A.M.L. and T.D.F. contributed equally to this work
aStanford University School of Medicine, Department of Radiology and Bio-X Program, The James H. Clark Center, 318 Campus Drive,
Clark E150, Stanford, CA 94305-5427, Phone: 650-725-2309, FAX: 650-897-9988
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers
we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting
proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could
affect the content, and all legal disclaimers that apply to the journal pertain.
NIH Public Access
Author Manuscript
J Mol Biol. Author manuscript; available in PMC 2009 June 22.
Published in final edited form as:
J Mol Biol. 2007 December 7; 374(4): 1017–1028. doi:10.1016/j.jmb.2007.09.078.
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In contrast, no similarity is seen between most of the coelenterazine using luciferases identified
so far (e. g. Gaussia, Renilla, Pleuromamma, Oplophorus) [4], even when the luciferases
originate from species within the same family (Gaussia versus Pleuromamma). This has been
taken to indicate that coelenterazine utilizing luciferases have emerged multiple times
throughout the course of evolution. Of the coelenterazine luciferases, the luciferase from
Renilla reniformis (RLuc) has been the most extensively studied [5] and the most widely
employed for research use. To date, however, no structure has been elucidated of any of the
coelenterazine using luciferases, including RLuc.
In Renilla reniformis, RLuc is found in membrane-bound intracellular structures within
specialized light emitting cells [6,7] along with two other proteins, a closely interacting green
fluorescent protein (RrGFP) [8], and a Ca++ activated luciferin binding protein (RrLBP) [9]
(the almost identical luciferin binding protein from Renilla mülleri has recently been
crystallized [10]). The chemical reaction that transpires in RLuc mediated bioluminescence
involves the catalytic degradation of coelenterazine and proceeds through a dioxetane (also
called dioxetanone or cyclic peroxide) intermediate step [11]. In vitro the reaction yields blue
light (480 nm peak), but in vivo it is not RLuc that is the light emitter but rather RrGFP. The
energy released by the luciferase catalyzed oxidation of coelenterazine is passed via resonance
energy transfer to the fluorophore of RrGFP and emitted as a green photon [12], explaining
the 505 nm peaked bioluminescence observed from the animal.
Since the cloning of the gene for RLuc [13] this luciferase has been widely used in molecular
biology, mainly as a reporter gene. More recently the gene has been incorporated into reporter
applications of increasing complexity, including fusion reporter genes [14–16], split-reporter
complementation systems [17], and resonance energy transfer based sensors [18,19]. Work has
also emerged utilizing variants of Renilla luciferase to create novel imaging probes by fusing
the luciferase to engineered antibodies [20] and to generate self-illuminating quantum dots by
attaching the luciferase as an internal light source [21]. In the development of these and other
applications, the tertiary structure of the protein would be helpful in understanding the most
effective way to employ the luciferase. For instance, structural data would allow assessment
of potential steric hindrance issues prior to the creation of fusion protein constructs involving
RLuc, and to assess which residues are available for site-specific conjugation reactions.
Structural data would also be helpful in guiding rational alteration of the enzyme in pursuit of
beneficial modification of its spectral and enzymatic properties [22,23].
The current work has focused on an 8 mutation variant of RLuc (RLuc8) in lieu of the native
enzyme, as this variant is both more stable and more easily expressed than the native enzyme
[22]. The protein's structure was successfully determined from crystals grown under a number
of different conditions, with a product bound structure highlighting residues that had previously
been found important for determining the enzyme's emission spectrum [23]. As RrGFP is
known to physically associate with RLuc in vitro, work with RrGFP was pursued as well.
RrGFP was found to be structurally very similar to the green fluorescent protein from Aequorea
victoria (AvGFP) with the exception of containing a much stronger dimerization interface.
Protein Characterization
Periplasmically expressed and purified RLuc8 was characterized by light scattering and
MALDI-TOF mass spectrometry to assess the oligomeric state and mass of the protein. Light
scattering results indicated that RLuc8 existed as a monomer in solution, as molar mass moment
calculations indicated a molecular weight of 33.8 kDa (error: 7%) with a relatively low
polydispersity across the gel filtration elution profile (~11%). By mass spectrometry, the
periplasmically expressed RLuc8 was measured as 36.8 kDa - within error of the expected size
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of 36.9 kDa. A minor peak around 38.5 kDa was noticed on some preparations, and may
indicate that the PelB signal peptide was not consistently processed and removed. This potential
issue was addressed by moving to the N-terminal cleavable 6xHis tag periplasmic expression
construct (S3RLuc8) as well as cytoplasmic expression constructs. Following thrombin
digestion and purification, mass spectrometry of the S3RLuc8 protein showed the desired
single peak.
One nicety of RrGFP purification, is that the level of purity attained can easily be monitored
by the ratio of the protein's OD498 to OD280. Higher ratio values imply greater purity and/or
a greater percentage of RrGFP in which the fluorophore has matured. The previously reported
ratio for pure RrGFP extracted from Renilla reniformis is OD498/OD280=5.6 [8]. Following
purification, the recombinantly produced RrGFP produced here had an OD498/OD280 ratio of
5.8. The ability of this 6xHis tagged RrGFP to interact and allow resonance energy transfer
with the 6xHis tagged RLuc8 was confirmed by combining the two proteins in 10 mM NaCl,
10 mM HEPES pH 7.4 at protein concentrations that precluded trivial transfer mechanisms
from playing a large role in the emission, and observing a green-peaked emission spectrum
matching the emission spectrum of RrGFP following the addition of coelenterazine (data not
shown, see [24]).
Crystallization Condition Screening
A large number of conditions as well as RLuc8 variants were evaluated, a full detailing of
which is available elsewhere [24] and only a pertinent synopsis follows. Conditions which led
to crystals used for X-ray diffraction are given in Table 1. Photographs of these crystals, along
with crystals from other conditions, are shown in Supplemental Figure 1. The initial
crystallization conditions identified, RLuc8:diammonium, RLuc8:KSCN,
S3RLuc8:thiomaltoside, led to high quality crystals. However, the growth time of the crystals
was on the order of 3–6 months, which microseeding failed to accelerate. Additionally,
coelenterazine and its analogs are sparingly soluble in aqueous solution, and a crystallization
condition was desired containing an organic component to allow an appreciable amount of
substrate to be included in the crystallization.
One semi-rational method employed to aid in crystallization is to switch a small number of
charged surface residues to alanines [25], with the rationale that removal of hydrophilic
residues reduces the desolvation associated entropy loss from formation of protein-protein
contacts. Generally, these mutations are done without knowledge of the tertiary structure of
the protein [26]. In our case, a structure had already been solved, so mutations were chosen
"intelligently" based on known crystal contacts. The residue pairs chosen were: K12A/E106A,
K25A/E277A, and E183A/K227A. All possible permutations of these pairs were expressed,
purified, and shown to retain at least 60% of the parental protein's activity (Supplemental Table
1). This work led to crystallization conditions (RLuc8/K25A/E277A:PEG/isopropanol,
RLuc8:PEG/isopropanol)) that allowed inclusion of appreciable amounts of substrate.
RrGFP was found to crystallize readily in a number of different mother liquors within time
scales of minutes to days. RrGFP will in fact slowly crystallize over several months when
stored in 10 mM NaCl, 10 mM HEPES pH 7.4. The best diffraction data was obtained with
the RrGFP:PEG/MPD condition.
Screening of RrGFP and cytoplasmically expressed RLuc8 in a 2:1 molar ratio (one RrGFP
dimer per RLuc8) produced co-crystals as demonstrated by gel electrophoresis. These crystals
were needle-like and of insuffcient size to achieve diffraction below 5 Å. Molecular
replacement using the RrGFP and RLuc8 structures on this low resolution co-crystal diffraction
data was unsuccessful.
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Structures of RLuc8
The structure derived from the RLuc8:diammonium condition is presented in Figure 1a, with
statistics for this as well as structures discussed later given in Table 2. Residues 4–308 (of 311
total) were successfully identified in the electron density data. Not identified were two residues
from the N-terminus, along with 3 residues on the C-terminus and the 6xHis tag. The resultant
structure from the RLuc8:KSCN condition was almost identical to that from
RLuc8:diammonium (Cα root mean square deviation 0.2 Å), with the sole exception that only
one of the imidazoles (corresponding to IMD1 in Figure 1a) was observable in the data.
The crystals from the S3RLuc8:thiomaltoside condition were in an orthorhombic setting (rather
than the hexagonal lattice observed for the diammonium phosphate and potassium thiocyanate
conditions), and contained two protomers in the asymmetric unit. Interestingly, the N-terminal
(residues 3–13) of one of the protomers in the asymmetric unit interacted with its neighboring
protein, turning away from the presumptive active site in doing so, contrary to the observation
in the diammonium phosphate structure. A superposition of the two monomers from this
condition with the previous RLuc8:diammonium structure, shown in Figure 1b, highlights this
change, as well as that the loop region containing residues 153–163 opened toward solvent.
These findings were taken to imply that these regions of RLuc8 are structurally flexible.
The crystal structure obtained from the RLuc8/K25A/E277A:PEG/isopropanol condition
lacked sufficient electron density to allow placement of a portion of the cap domain (residues
153–162), which again would be consistent with flexibility in this region of the protein. In
addition to these missing residues, neither substrate nor product could be identified in the
electron density. Unexpectedly, the surface mutations (K25A, E277A) made in this construct
with the intention that they would aid in crystallization were not involved in contacts between
proteins in this crystal.
Further screening led to RLuc8:PEG/isopropanol, which successfully yielded electron density
for the reaction product coelenteramide in difference maps of the active site (Figure 1c),
although the density in parts of the coelenteramide structure appear weak suggesting disorder
of either static or dynamic nature. The locations of residues 153–154 were disordered as well
and were not included in the model.
A possible explanation for the preference of benzyl-coelenterazine in crystallizing RLuc8/
K25A/E277A versus coelenterazine in crystallizing RLuc8, can be proposed based on the
interactions of coelenteramide with the protein's residues as shown in Figure 1c. This closeup
shows that a hydroxyl group in coelenteramide interacts with E277 through a predicted
hydrogen bond. In RLuc8/K25A/E277A, this glutamate has been mutated to alanine and the
predicted hydrogen bond cannot form. Benzyl-coelenterazine lacks this hydroxyl group;
presumably there is a favorable hydrophobic interaction between A277 and the hydroxyl-
lacking benzene ring.
Variants of the protein RLuc8/K25A/E277A were created containing alanine substitutions at
the presumptive catalytic triad residues D120, E144, or H285 [22], with the hope that these
potentially inactivating mutations would allow crystallization of the protein in complex with
substrate rather than product. However, neither coelenterazine nor coelenteramide was
observed in the resulting electron density maps derived from these crystals.
Structure of RrGFP
The resulting structure from X-ray diffraction of the RrGFP:PEG/MPD crystallization
condition is presented in Figure 2a, with the corresponding statistics given in Table 2. In the
obtained structure of RrGFP, the initial 6 and last 7 amino acids of the primary sequence could
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not be located in the electron density. Although the electron density maps were of very high
quality, the R factors were unusually high (~30%), suggesting some type of disorder or
deviation from ideality that could not be modeled using translation/libration/screw (TLS)
models for each protomer [27] - one such possibility is the high solvent B factor (~69) and low
Wilson B (~14), suggesting a significant amount of solvent disorder relative to protein.
The tertiary fold pattern seen is the classic β-barrel characteristic of the fluorescent proteins,
and the expected fluorophore of p-hydroxybenzylidene-imid-azolidone was readily apparent
in the electron density data (Figure 2c) [28,29]. A comparison of RrGFP and AvGFP is shown
in Figure 2b. Unsurprisingly given the close primary sequence similarity between RrGFP and
AvGFP (50% similar, 28% identical), the resultant structure for RrGFP is analogous to the
previously known structure for AvGFP (Cα root mean square deviation 1.4 Å).
RrGFP is known to be a dimer [8]. Accessible surface area (ASA) calculations for the interface
between the protomers I and II (equivalently III and IV) in Figure 2a yield an interface interface
ASA of 1316 Å2 and 14 hydrogen bonds, appropriate values for a dimerization interface
[30]. In contrast, the other interface in the crystallographic unit (I–III, or II–IV) had an interface
ASA of 156 Å2 and 1 hydrogen bond. For comparison, AvGFP, which is known to weakly
dimerize [31], has an ASA of 848 Å2, and 8 hydrogen bonds on the interface corresponding
to the dimerization interface of RrGFP.
Much like the similar bacterial haloalkane dehalogenase [32], Renilla luciferase has a
characteristic α/β-hydrolase fold sequence at its core [33] and shares the conserved catalytic
triad of residues employed by the dehalogenases [22]. The level of primary sequence similarity
is somewhat surprising given that the dehalogenases are hydrolases while the luciferase is an
oxidase, and hypotheses on how this situation may have came to be from an evolutionary
standpoint have previously been discussed [22]. Now with the structure of the luciferase, the
high level of tertiary structure similarity to the haloalkane dehalogenases can be noted as well
(Cα root mean square deviations ~1.5 Å for LinB structures). A topological map of the RLuc8
α/β-hydrolase fold is shown in Figure 3a, along with the locations of the presumptive catalytic
triad residues D120/E144/H285A within this diagram. α/β-hydrolases have their nucleophile
(D120 in RLuc) immediately after the fifth β-sheet (β5) in what is termed the "nucleophile
elbow". The sequence pattern for this elbow is generally G-X-Nuc-X-G [34] in the α/β-
hydrolases, and corresponds to GHDWG (residues 118–122) in Renilla.
A feature observable in the RLuc8 structures (Figure 1a) that was not apparent in previous
homology models is the wrapping of the N-terminus around the protein toward the front of the
presumptive enzymatic pocket. There was also occasional variability in the placement of the
initial ~10–15 residues (e. g. monomer 1 in Figure 1b), indicating that this region of the protein
may contain a high degree of conformational flexibility. Variants of RLuc8 with the N-terminal
clipped up to position I15 are relatively well tolerated and retain >25% of activity (data not
shown, see [24]), demonstrating that this N-terminal region is not required for enzymatic
activity of the protein. It has been a general observation in our laboratory that fusion proteins
created by attachment to the N-terminus of RLuc have a greater propensity towards low
luciferase activity than fusions made by attachment to the C-terminus of RLuc. Based on the
structural data and the non-essentialness of the N-terminal region from an enzymatic
standpoint, it can be hypothesized that this drop in activity for N-terminal fusions is due to
steric hindrance of the RLuc active site.
In the RLuc8:diammonium structure two imidazole molecules, apparently from the mother
liquor, were located in the presumptive catalytic pocket of the molecule. Previous reports
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text:
Currently 76.5 °F
October 8th, 2015
9:03PM on Thursday 26th June 2014
( 1 year ago )
Braves miss out on sweep of Astros with 6-1 loss
The Associated Press
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HOUSTON -- The Houston Astros had solid pitching but couldn't get their offense going in the first two games against Atlanta.

 On Thursday, they finally put together a complete game and won 6-1 to avoid a series sweep.

 Matt Dominguez broke out of a slump with a three-run home run and rookie George Springer also homered to back up seven strong innings by Jarred Cosart.

 Dominguez snapped an 0-for-23 skid with his two-out shot to left-center field off Mike Minor (2-5) that made it 5-1 in the fifth inning.

 "That's the big hit that when we were in this here funk that we were in, that was the big hit that had been eluding us," manager Bo Porter said.

 Dominguez said there was a mixture of relief and excitement when he connected on his home run.

 "I try not to focus on it but of course it gets in your head," Dominguez said. "Good situation. Got a good pitch to hit and I was finally able to put the barrel on it."

 Cosart (8-5) allowed six hits and a run for his fourth straight victory. Porter believes that Cosart's presence on the mound has improved with each start this season and the 24-year-old agreed.

 "When I'm throwing strikes I have a lot more confidence and obviously that's what they've been preaching," Cosart said. "And with the mechanical stuff I've been doing I have a lot more confidence in throwing strikes and just being able to trust my stuff and attack the hitters and know I've got a really good defense behind me that's going to make a lot of plays for me."

 Springer's solo homer off Juan Jaime in the seventh pushed the lead to 6-1. He has 15 homers this season, the most by a rookie in franchise history before the All-Star Break. Lance Berkman had the previous mark with 12 in 2000.

 Minor gave up seven hits and five runs in six innings to remain winless since May 19.

 Jon Singleton had two hits and an RBI and Jose Altuve added a pair of hits and swiped two bases for the Astros, who had managed just four runs combined and had been shut out twice in a four-game losing streak. Altuve leads the American League with 107 hits and 30 steals.

 Justin Upton had two hits on Thursday and hit .364 with two homers and four RBIs in the series and B.J. Upton had a hit and scored Atlanta's only run.

 "Things just didn't go our way," Justin Upton said. "For the most part we tried to play a little small ball with our run-scoring struggles and we've done a pretty good job of it. (Thursday) it didn't work out for us and we didn't get the big ball, either."

 The Braves had trouble stringing together hits against Cosart and he was helped by the Astros turning a pair of double plays. Evan Gattis doubled to start the seventh before Jason Heyward drew a walk, but Cosart retired the next three batters to end the threat.

 B.J. Upton hit a leadoff single before stealing second and taking third on an error on the throw by Jason Castro. The Braves went up 1-0 when he scored on a sacrifice fly by Tony La Stella.

 Altuve singled and a double by Singleton, which bounced off the wall in left field, sent him home to tie it at 1-1 in the first.

 Minor plunked Dominguez to start Houston's fourth before a double by Castro. The Astros took a 2-1 lead when Castro scored on a groundout by Jesus Guzman.

 NOTES: The Astros opened their gates 30 minutes early Thursday to allow fans to watch the World Cup match between the United States and Germany on the stadium's big screens. ... Atlanta 1B Freddie Freeman was back in the lineup after leaving Wednesday night's game in the eighth inning after being hit by a pitch on the right elbow. ... Houston selected the contract of RHP Jose Veras from Triple-A Oklahoma City and outrighted RHP Kyle Farnsworth before the game and he has elected free agency. Veras struck out Justin Upton for the last out of the game.
Associated Categories
Professional Sports
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All rights reserved. This material may not be published, broadcast, rewritten, or redistributed without permission.
2014 Top 10: Looking back at the top sports stories of the year
It was another year for the record books in 2014, mostly for the right reasons -- state championships, new challenges and new stars taking their turn to shine. But there were also plenty of big stories that reminded us that sports -- like life -- isn
11:55PM ( 9 months ago )
NASCAR K&N Pro Series schedules set for 2015
DAYTONA BEACH, FL - NASCAR announced recently the 2015 schedules for its top developmental regional touring series, the NASCAR K&N Pro Series East and NASCAR K&N Pro Series West.
2:00PM ( 9 months ago )
Lanierland boys final: Johnson makes history with win over Red Elephants  (VIDEO)
Ever the aggressor on the court, Johnson
8:55AM ( 9 months ago )
Lanierland girls final: Lady Elephants hold off Branch to repeat as champs  (VIDEO)
Chubb, Dogs run over Louisville, 37-14
Boys basketball: Tigers snap losing streak with in Mountain Top Classic
Millsap powers Hawks past Cavaliers, 109-101
Georges-Hunt leads Tech past 49ers 67-66
Girls basketball: Banks County wins Commerce Holiday tourney
Girls basketball: Buford takes 3rd in Farm Bureau Classic
Boys basketball: Banks Co. boys beat Madison Co, take Commerce tourney title
Pits will be tight as Chili Bowl entry count hits 316
TULSA, OK - The 29th annual Lucas Oil Chili Bowl Midget Nationals presented by General Tire is on track to be a record smashing event, with the entry count climbing over the 300 mark for the first time to 316 entries confirming 303 drivers for the indoor spectacular happening Tuesday, January 13 through Saturday, January 17 at the Tulsa Expo Center in Tulsa, OK.
9:59AM ( 9 months ago )
Falcons fire Mike Smith after 2nd straight losing season
Mike Smith's status as the winningest coach in Falcons history wasn't enough to save his job. Owner Arthur Blank said he fired Smith on Monday because in his "heart of hearts" he didn't believe Smith could take the Falcons to the Super Bowl.
7:58PM ( 9 months ago )
New promoters in place for 2015 at Senoia Raceway
SENOIA, GA - Lease papers have been signed and a team of new promoters is in place for the upcoming stock car racing season at Senoia Raceway in Senoia, GA.
6:00AM ( 9 months ago )
Professional Sports
Flowery Branch's Shaw throws for 177 yards in NFL debut
Panthers crush Falcons 34-3, roll into playoffs
Hawks beat Bucks to split home-and-home series
Gladiators outduel Road Warriors
Flowery Branch's Conner Shaw to start Sunday for Cleveland Browns
Falcons boosted by Julio Jones' return to practice
Perfect 10: Dudley doesn't miss, Bucks rout Hawks
Gladiators fall to Road Warriors
Surprising Tigers looking to take early lead in 7-AAA
Sid Maxwell admitted he wasn't sure what he was getting into when he decided to take the Dawson County job back in the spring.
Jeff Hart
8:02PM ( 1 hour from now )
Falcons downplay White's complaints about lack of passes
Atlanta Falcons receiver Roddy White skipped practice Wednesday for an undisclosed personal matter, while the team addressed concerns that he's not happy with a diminished role in the offense.
The Associated Press
8:42AM ( 9 hours ago )
VIDEO: Kevin Harvick, Regan Smith tame the 'Monster Mile'
If there's one thing the NASCAR world should know by now, it's that Kevin Harvick is hard to beat when he's in a must win situation.
Brandon Reed
8:00AM ( 10 hours ago )
Tennessee dismisses WR Pig Howard ahead of facing UGA
Tennessee wide receiver Alton "Pig" Howard has been dismissed from the team for a violation of unspecified team rules.
The Associated Press
5:48AM ( 12 hours ago )
Cross country: Johnson boys, North Hall girls claim Hall County titles
Johnson's boys took first place individually and as a team, while North Hall's girls did the same on Wednesday at the Hall County Cross County Championships at Chestatee High in Gainesville.
AccessWDUN Staff
12:12AM ( 18 hours ago )
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51 members
641 members
78 members
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109 members
Thanks Freda... happy new year to you too.. already seeing bulbs come up here!
Freda, you catch the beauty in everything............................
Freda - you can certainly paint the butterfly. If you want me to e-mail you a higher resolution jpeg I can do that too.
Cathy Hennessy


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Re: Fico Score did not improve after 7 month credit card activity
I know accounts in dispute don't count in utilization, but was surprised that a simple comment could leave it in limbo for so long. But good it got resolved.
As far as the short credit history is concerned, I suspect it may be caused by a card reporting a positive balance when it hasn't for a while or never.
Never underestimate the power of denial
(1,304 Views)


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chevelle - Page 2
Chevelle Unveil Artwork + Track Listing for Upcoming Album 'La Gargola'
Chevelle are giving fans yet another reason to be excited about their new album. The next step leading up to the release of the 'La Gargola' album is the band unveiling the artwork and track listing for their new disc.
Chevelle's Sam Loeffler Talks 'La Gargola' Album + 'More Cowbell' With 'Loudwire Reloaded'
Chevelle are back with a new album, 'La Gargola,' due April 1. The disc has already generated the new single 'Take Out the Gunman,' and we'll be playing that song on this weekend's 'Loudwire Reloaded' radio show. Host Sandman spoke with Chevelle drummer Sam Loeffler about the band's upcoming disc and you can read the interview below and catch a portion of the chat on this weekend's 'Loudwire Reloaded' show.
Chevelle, In-Studio Album Preview – Exclusive Video
The year 2014 is looking like it's going to be a big one for Chevelle! The band just announced that their new album is titled 'La Gargola' and will hit stores April 1. Giving fans another taste of what's to come, the members of Chevelle shot this behind-the-scenes look at the making of their new album and Loudwire is giving you the exclusive premiere (watch above). Moreover, you can check out the band's brand-new single 'Take Out the Gunman' below.
Chevelle Hit Studio To Work on New Album, Offer Video Teaser
Chevelle Frontman Pete Loeffler Injures Knee in Frightening Stage Fall
Chevelle Hit West Coast for Fall 2012 Tour
Chevelle just keep on chugging along with their latest album, 'Hats Off to the Bull.' The Windy City rockers are currently one of the top draws on the Carnival of Madness tour, and have just announced their first post-Madness dates for the fall.
Chevelle Unveil Cover Art, Track Listing for New Album 'Hats Off to the Bull'
Chevelle Announces Release Date for New Album and New Song, 'Face to the Floor' [VIDEO]
Back in the day, the release of a concert album used to be a sure sign that a band was not going to be putting out a new studio effort anytime soon.
Chevelle fans can rest easy, however. Even though the Chicago-bred group released the live collection 'Any Last Words' in the spring, a new studio disc is on the way, just in time for the year-end holiday season.
Abrupt Edge and Seven Cycles To Open for Chevelle
Chevelle is set to rock Tri-Cities on August 29th. They've just announced 2 bands opening up for them. Abrupt Edge and Seven Cycles!
Chevelle To Rock Tri-Cities on August 29th
Chevelle was set to play along with the rest of the Carnival of Madness tour but had to back out at the last second. They were so bummed about it they wanted to make up for it by performing just by themselves in a special concert. Tickets are on sale now and we've got your chance to win, too!
Chevelle Live in Spokane on August 24th
Chevelle is set to rock Spokane on August 24th. If you wanna go, we've got your tickets!


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Civil Service Fund:
Improved Controls Needed Over Investments
AFMD-87-17: Published: May 7, 1987. Publicly Released: Jun 10, 1987.
Additional Materials:
- Full Report:
(202) 275-9454
Office of Public Affairs
(202) 512-4800
Pursuant to a congressional request, GAO reviewed the Department of the Treasury's management of the Civil Service Retirement and Disability Trust Fund during debt ceiling crises in 1984 and 1985 to determine how much interest Treasury lost by not following its normal securities investment and redemption policies.
GAO found that: (1) during two periods when the federal debt was near its legal limit, Treasury departed from its normal procedures by delaying investment of fund receipts or prematurely redeeming fund securities; (2) the unintentional consequences of Treasury's actions cost the fund about $160 million; and (3) although Congress enacted legislation to permit Treasury to reimburse the fund and its intent was to fully restore the losses, the legislative language was somewhat restrictive and the fund's future earnings could ultimately decrease by up to $13.6 million. GAO also found internal control weaknesses not related to the debt ceiling, in that: (1) adjustments to the fund's accounting records were not adequately documented and did not ensure the accuracy of interest earnings; and (2) the Office of Personnel Management (OPM) underreported to Treasury the amounts to be invested on the fund's behalf. Treasury cannot restore the losses resulting from those weaknesses because of statutory limitations. In addition, GAO found that Treasury has not evaluated the system used to account for fund investments for conformance with applicable accounting principles and standards, as required by the Federal Managers' Financial Integrity Act.
Recommendations for Executive Action
Status: Closed - Implemented
Comments: Treasury is reviewing an analysis of the losses, and when the amount is determined, Treasury will sponsor appropriate legislation to restore the losses.
Recommendation: The Secretary of the Treasury should direct the Financial Management Service to determine the amount of unrestored losses resulting from the 1984 and 1985 debt ceiling crises and seek the necessary legislative authority to fully restore those losses to the fund.
Agency Affected: Department of the Treasury
Status: Closed - Implemented
Comments: Treasury controls adjustments and redemptions and, during the FIA review of the Public Debt Accounting System, will review internal controls over investment operations. Treasury noted that any legislation should include all trust funds and preserves equity between the trust funds and the general fund. Since such legislation would have broad impact, it may take 12 to 18 months for final passage.
Recommendation: The Secretary of the Treasury should direct the Financial Management Service to: (1) document properly all adjustments to the accounting records; (2) establish controls to ensure that the lowest-interest-rate securities are redeemed first; and (3) evaluate the fund's accounting operations for conformance with applicable accounting principles and standards. The Secretary should also seek legislative authority to fully reimburse the fund for losses resulting from future Treasury or OPM management of fund transactions.
Agency Affected: Department of the Treasury
Explore the full database of GAO's Open Recommendations »
Sep 23, 2015
Corporate Bankruptcy:Stakeholders Have Mixed Views on Attorneys' Fee Guidelines and Venue Selection for Large Chapter 11 CasesGAO-15-839: Published: Sep 23, 2015. Publicly Released: Sep 23, 2015.
Sep 15, 2015
Confidential Informants:Updates to Policy and Additional Guidance Would Improve Oversight by DOJ and DHS AgenciesGAO-15-807: Published: Sep 15, 2015. Publicly Released: Sep 15, 2015.
Jun 29, 2015
Youth Athletes:Sports Programs' Guidance, Practices, and Policies to Help Prevent and Respond to Sexual AbuseGAO-15-418: Published: May 29, 2015. Publicly Released: Jun 29, 2015.
Jun 19, 2015
Federal Prison System:Justice Could Better Measure Progress Addressing Incarceration ChallengesGAO-15-454: Published: Jun 19, 2015. Publicly Released: Jun 19, 2015.
May 28, 2015
U.S. Parole Commission:Number of Offenders under Its Jurisdiction Has Declined; Transferring Its Jurisdiction for D.C. Offenders Would Pose ChallengesGAO-15-359: Published: May 28, 2015. Publicly Released: May 28, 2015.
Apr 29, 2015
Victims of Child Abuse Act:Further Actions Needed to Ensure Timely Use of Grant Funds and Assess Grantee PerformanceGAO-15-351: Published: Apr 29, 2015. Publicly Released: Apr 29, 2015.
Mar 23, 2015
Department of Justice:Alternative Sources of Funding Are a Key Source of Budgetary Resources and Could Be Better ManagedGAO-15-48: Published: Feb 19, 2015. Publicly Released: Mar 23, 2015.
Mar 16, 2015
Criminal History Records:Additional Actions Could Enhance the Completeness of Records Used For Employment-Related Background ChecksGAO-15-162: Published: Feb 12, 2015. Publicly Released: Mar 16, 2015.
Mar 4, 2015
Whistleblower Protection:Additional Actions Needed to Improve DOJ's Handling of FBI Retaliation ComplaintsGAO-15-343T: Published: Mar 4, 2015. Publicly Released: Mar 4, 2015.
Feb 23, 2015
Whistleblower Protection:Additional Actions Needed to Improve DOJ's Handling of FBI Retaliation ComplaintsGAO-15-112: Published: Jan 23, 2015. Publicly Released: Feb 23, 2015.
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Rap Superstar T-Pain (Low, Good Life)
Full Price
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Two-time Grammy-winning rap star T-Pain stops at Tru Hollywood on his I Am T-Pain tour armed with his biggest hits, like Low and Good Life, and newest tracks, including Up Down, from his upcoming fifth solo album, Stoicville: The Phoenix. Known for his signature Auto-Tune sound, T-Pain has worked work everyone from Kanye West and Lil Wayne to Chris Brown and Jamie Foxx. He's been nominated and won a string of awards and, incredibly, he was featured on more than 50 chart-topping singles in a period of five years -- from 2006 to 2010.
* Additional fees apply. No coupon or promo codes necessary to enjoy the displayed discount price.
All offers for T-Pain have expired.
The last date listed for T-Pain was Sunday April 6, 2014 / 8:00pm.
Most Popular Music Event Nearby
Ramón Ayala: Norteño's "King of the Accordion" Returns to Long Beach
- Full Price:
- $45.00 - $150.00
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- $25.00 - $99.00
Known as the "King of the Accordion," American and Latin Grammy Award-winner Ramón Ayala has… More
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Featured review from Bernard Gordon Jr
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Been to three Sean Healy events...hasn't let me down yet! Keep up the good work!
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Doors: 8:00pm
Tour Headliner: 10:00pm
VIP Admission includes priority entry and access to VIP Lounge.


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NASBA names director of legislative affairs
HCA Florida hospitals unionize
National Nurses United officials are basking in the glow of a wide-ranging labor deal they've signed with HCA executives in Florida. The new contract covers more than 3,000 nurses at 10 hospitals across the Sunshine State. Union leaders plan to leverage the deal in other parts of the country.
RNs say the settlement could influence negotiations at 6 other HCA hospitals in Texas and Missouri where NNU affiliates are also in first contract talks. In December, 2011, 450 RNs at HCA's MountainView Hospital in Las Vegas, members of NNU's Nevada affiliate, reached agreement on their first contract. HCA RNs at two San Jose, CA, hospitals begin their negotiations for contract renewals this month. Menorah Medical Center RNs in Kansas will do the same this summer.
Sitel opens Florida call center
Sitel today announced the opening of a new call center in Ocala City, Fla.
The center is expected to hire 500 customer support agents.
"This is a very exciting time for Sitel as we grow our North American footprint and continue to mean more to our clients, shareholders, associates and communities," Judy Morris, Sitel vice president of human resources for the Americas, said in a release. "We are moving quickly and establishing a strong managerial staff to lead this new site."
Nashville-based Sitel is a business process outsourcing provider with an international presence.
Healthcare Realty unloads some Florida properties
In their first-quarter earnings report Wednesday, Healthcare Realty Trust executives said they had spent $21.3 million on acquisitions. The Nashville-based real estate investment trust has since recouped that money and then some by selling some of its properties in Florida.
Healthcare Realty last month sold to Texas-based MedProperties and AW Property out of Palm Beach medical office buildings in the Tampa area as well as farther south in Venice, Bonita Springs and Fort Myers. Combined, the buildings have almost 240,000 square feet.
Separately, the company also sold to AW and New York-based HRE Capital a 34,500-square-foot MOB in Lehigh Acres. In all, the sales put $33.3 million in Healthcare Realty's bank account, but the company — which owns another dozen or so properties in Florida — said in its quarterly filing with the Securities and Exchange Commission that it expects to book a small impairment charge on the deal.
Servpro accuses Florida competitor of trademark infringement
HCA taps new West Florida president
HCA announced today that it has named Peter Marmerstein as its new West Florida Division president, succeeding Dan Miller.
"Peter is a veteran hospital administrator with more than 30 years experience in multiple settings," said Chuck Hall, President of HCA's National Group. "He has a proven track record and has done a great job leading the integration of the two CJW campuses while continuing to foster growth and expand their services. We are pleased to welcome him to the West Florida Division"
In his new position, Marmerstein will oversee "16 hospitals and 16 surgery centers on the west coast of Florida and near Orlando."
Florida prison privatization plan voted down
Nashville-based Corrections Corp. of America and its main competitor GEO Group won't have a chance to pick up hundreds of millions of dollars in new Florida business after legislators there voted against a plan to privatize more than 20 prisons. GEO's CEO had called the proposal the private prison sector's biggest-ever contract opportunity. Shares of CCA (Ticker: CXW) have held steady over the past few days and are up about 11 percent in the past three months. The company last week beat analysts' estimates with its fourth-quarter earnings.
Big Florida prison privatization plan hangs in balance
Florida lawmakers are rapidly approaching a showdown vote on the large prison privatization plan that would give Corrections Corp. of America the chance to win some big new contracts in the southern half of the state. State senators on Monday narrowly voted against an amendment to the plan. But it looks like a final vote on the proposal will result in a deadlock.
SEE ALSO: Florida moves closer to big prison privatization from late last month
Label exec Stroud responds to financial woes report
Florida moves closer to big prison privatization
A plan to privatize about 30 prisons in the southern part of Florida is headed to that state's Senate soon. The proposal, which has met with opposition from labor unions and some lawmakers, would put Nashville-based Corrections Corporation of America in position to secure many new contracts.


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Search the Collection
Sitter in 2 portraits
by Elliott & Fry
by Elliott & Fry
Sir Donald Kaberry, 1st Bt(1907-1991), Politician; MP for Leeds
Sitter in 2 portraits
by Elliott & Fry
bromide print, 10 May 1952
NPG x90076
by Elliott & Fry
quarter-plate glass negative, 10 May 1952
NPG x100462
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Haviland Pool & Spa Products, a chemical manufacturer in Grand Rapids, Mich., has promoted Misti Daly to the position of customer service manager. As chair of Haviland's Customer Satisfaction Council, she also assists all sales and technical personnel in achieving customer satisfaction. Prior to joining Haviland, Daly worked for 16 years at a small chemical distributor in a number of positions.
Customer Service
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Portable spa installers must follow special precautions when selecting and preparing...
Top 50 Builder Dennis Carson of Premier Pools of Los Angeles shares his thoughts on the...
Welcome to the inaugural Pool & Spa News podacast, Top 50 Builder Bites, in which Erin...
Joanne McClain
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45 year old woman | Reno, NV, United States | Seeking men 36-45 within 50 miles
Active within 24 hours
Girl that loves the outdoors
I am grateful everyday for my health, my close friends, life lessons, my work and moments when I cannot catch my breath from laughing. I love my sons and work hard to be a good mom and role model. I like doing anything healthy and active. And yes, wine is healthy, in the right dose of course. My favorites are bike riding and kayaking but I love doing anything on or by water and outside really. WANTED: an incredibly playful man that I am able to say exactly what I feel, good or bad, without wondering how to phrase it. A gentle soul that when you're around creates a safe space to my life that I am okay being vulnerable in. Laughter is part of our everyday conversation with playful sarcasm. You are kind and strong in your own way, spiritual without being judgmental. Willing to grow on all levels. Ready to assist me in my desire to give back to the community. Oh yah and great at a foot rub would be an awesome bonus. No wonder you are hard to find. In the meantime I will continue to play with wonderful friends & family until you are ready for me :)
Camping , Coffee and conversation , Business networking , Dining out , Gardening/Landscaping , Hobbies and crafts , Museums and art , Music and concerts , Exploring new areas , Playing sports , Travel/Sightseeing , Volunteering , Wine tasting
Cycling, Dancing, Golf, Other types of exercise, Skiing, Tennis / Racquet sports, VolleyBall, Walking / Hiking, Weights / Machines, Yoga
I exercise 3-4 times per week.
I have Dogs
I like Birds, Fish, Horses
My dog is a tiny mutt that looks like a monkey.
San Diego, Big Sur, any coast, Friday eve music by the river, Sundays at Commons Beach, Old Silver Peak, Vino 100, Coffee Bar, The Hub Coffee
Fun is more about the company. I look for fun everywhere! I do love outdoor music and being on a bike or in a kayak , etc etc etc
Business textbooks, proverbs
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'11" (180cms)
|5'11" (180cms) to 8'11" (271cms)
|Body type:
|I'll tell you later
|About average, Athletic and toned
|I'll tell you later
|No preference
|I'll tell you later
|No preference
|No Way
|No Way
|No preference
|Self-Employed / Entrepreneur
|No preference
|I'll tell you later
|No preference
|No preference
|Have kids:
|Yes, and they live at home (1)
|No preference
|Wants kids:
|No, but it's OK if my partner has kids
|No preference
|Native American, White / Caucasian, Other
|No preference
|Christian / Other
|Christian / Protestant, Spiritual but not religious, Other, Christian / Other
|Associates degree
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Sold by: BasementBrands
Seller Rating: (302)
|$9.43 & FREE Shipping on orders over $35. Details
Manufactured using high carbon steel and high quality chrome vanadium steel for strength, durability and resistance to corrosion. These tools feature ID stamping showing size and brand.
The Goldblatt Neon Handle knives are color coded to size making them easy to locate on the jobsite. These quality and lightweight finish knives have been the choice of the pro for decades. Large-grip plastic handles and finger-rests make them easy to use and maneuver. Flexible blue steel blade gives the perfect finish. Available in 6-Inch, 8-Inch, 10-Inch and 12-Inch color-coded sizes.


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Visit Italy on an adventure tour from Rome to Amalfi
If you want a short trip packed with diversity, then this tour of southern Italy is the adventure for you. Acquaint yourself with Italy's 'Eternal City' of Rome and learn about the catastrophic fate of Pompeii. Soak up the sunshine in the coastal town of Sorrento and explore the beautiful Amalfi Coast. This trip offers the chance to delve into history, culture and cuisine and to live a little of la dolce vita.
- Explore the sights of beautiful Rome
- Sip espresso with sharp-dressed locals
- Sample authentic Neapolitan pizza
- Ponder the power of nature in Pompeii
- Trek up Mt Vesuvius
- Hike among the olive groves and terraces of the Amalfi Coast
- Toast the adventure with a sweet limoncello
Day 1 Rome
Day 2-3 Naples/Pompeii
Day 4-5 Sorrento
Day 6-8 Amalfi
Meals2 breakfasts
TransportBus, Metro, Train
AccommodationHotel (7 nights)
Included activities
- Archaeological Museum
- Gelato Making Demonstration
- Guided Walk Pompeii
- Mt Vesuvius summit climb
- Old Pompeii Entrance
There are currently no departures for this trip.
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If you can't find the dates you're after here, click on the button below to find options for the next season. Please bear in mind that some of our itineraries can change from year to year, so this trip may differ slightly next season to the information shown on this page.Next Season Dates
Important notes
Trip notes
Want an in-depth insight into this trip? Your trip notes provide a detailed itinerary, visa info, how to get to your hotel, what's included - pretty much everything you need to know about this adventure and more.View trip notes
Our Rome to Amalfi trips score an average of 4.81 out of 5 based on 54 reviews in the last year.
Rome to Amalfi , September 2015
Sarah Cox
Living la dolce vita! A pleasant 2 week trip ticking off the sites of Italy. Although take more money than you think you need - you'll need more spending money just to eat out twice a day! Whatever you do, make time to go and visit Herculaneum as well. It is incredible. Much tinier than Pompeii, but so much better preserved and hardly any tourists go there compared to Pompeii. The ruins are so much better preserved as they were buried under so much mud. A few of us on the trip did it in the same day as Pompeii and it was absolutely so worth it. It is about 20 mins on a train from Pompeii. Also, if you are looking forward to Rome at the half way stop, the group will only get into Rome early afternoon. I booked my own train ticket and left for Rome at very early in the morning without the group (I just had to sign a disclaimer form, which our tour leader gave me). The hotel in Assisi was less than 5 minutes walk from the train station, which was so convenient. This mean that I could get a 07.03 DIRECT train and be in Rome for 9am. Then have 2 full days in Rome, not just 1 and a bit days which is never enough time. It was interesting to go through Naples as well, glad we did. And yes, it is exactly as you expect it to be - rough around the edges, seedy, grimy, graffiti everywhere. So it didn't disappoint. Our tour leader took us to the best pizza place in Naples, where the locals queue outside. Best pizza I have ever had!! Be prepared to be wowed by the Pompeii mosaics that are in the Naples Museum - incredible!
Review submitted 12 Oct 2015
Rome to Amalfi , September 2015
Candice Pope
Rome to amalfi tour has a mix of everything! History, culture, walking up a volcano, amazing opportunities to eat local Italian food to relaxing on the beach and soaking up the sunshine. Definitely stay a couple of extra nights in amalfi after, you won't regret it!
Review submitted 30 Sep 2015


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Sep 20
We Will Be Promoting Our Debut Album 'Jail Time Credit' At One Of Our Favorite Spots In The City and jamming with our friends Rogues Cross.
Apr 9
The first Rogues Cross show in over 7 years! Don't miss out!