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This webinar discusses the patchwork policies that states have on unauthorized immigrants and in-state tuition, financial aid and other benefits, and the implications for unauthorized immigrant youth seeking two- and four-year college degrees.
MPI Webinar
This event explores the social and health impacts of international labour migration on the children who remain at home when one or both their parents emigrate.
Report release where authors discuss the effects of parental deportation on the children of immigrants, the related needs for health and social services, and U.S. policy responses to protect these children.
A webinar/call with senior officials from some of the EU Member States to discuss their differing perspectives on the current refugee crisis.
A webinar examining aspects of the implementation at state and local levels of the federal Workforce Innovation and Opportunity Act (WIOA) that may limit immigrant integration, along with a discussion on strategies that may help ensure more equitable access for immigrants and refugees to services provided under the law.
In this webinar, researchers will explore the educational, psychological, and social impact of discrimination on immigrant-origin children from birth to age 10.
MPI held a ceremony and awards program, honoring visionaries in immigration policy in the United States and internationally. The ceremony opened with comments from MPI President Demetrios Papademetriou; featured a discussion between MPI Senior Fellow Doris Meissner and former Rep. Ron Mazzoli, one of the lead sponsors of the 1986 Immigration Reform and Control Act; and included a keynote address by Open Society Foundations President Aryeh Neier.
A discussion in Brussels, upon the official launch of Migration Policy Institute Europe, to explore what is driving societal discontent in Europe, the role immigration plays in this, and why there is a growing perception that immigrant integration efforts are failing.
A discussion on the use of prosecutorial discretion and the review by U.S. Immigration and Customs Enforcement (ICE) of all current cases in the removal pipeline and on the docket of immigration courts, with U.S. Department of Homeland Security's Deputy General Counsel; the Director of the Executive Office for Immigration Review in the U.S. Department of Justice; and ICE's Director of Field Legal Operations in the Office of the Principal Legal Advisor.
A discussion on the gains that young adult immigrants or the U.S.-born children of immigrants have made in education and employment.
This webinar from the MPI's National Center on Immigrant Integration Policy and the U.S. Conference of Catholic Bishops' Bridging Refugee Youth & Children's Services program explores federal requirements for providing interpretation and translation in schools and how select school districts in Minnesota and Colorado have managed these requirements.


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Becky has spent her entire career cooking and baking, which are also her favorite hobbies outside of work. For her, time spent in the kitchen not only relieves stress, but is lots of fun as well. Becky's love for baking began as a child, standing on a chair next her mom, adding ingredients and mixing up dough.
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Tea and cookies are made to enjoy together. These delicate wafer cookies are filled with buttercream for a dainty tea time treat.
Combine flour, butter and whipping cream in bowl. Beat at low speed, scraping bowl often, until well mixed.
Divide dough into thirds. Wrap each third in plastic food wrap; flatten slightly. Refrigerate 2 hours or until firm.
Heat oven to 375° F.
Roll out dough on well-floured surface, one-third at a time (keeping remaining dough refrigerated), to 1/8-inch thickness. Cut with 1 1/2-inch round cookie cutter. Dip both sides of each cookie in sugar. Place 1 inch apart onto ungreased cookie sheets; prick all over with fork.
Bake 6-9 minutes or until slightly puffy but not browned. Cool slightly; carefully remove from cookie sheets. Cool completely.
Combine all filling ingredients except milk or juice and food color in bowl. Beat at medium speed, gradually adding enough milk for desired spreading consistency. Tint filling with food color, if desired. Carefully put cookies together using 1/2 teaspoon filling for each sandwich cookie.
Calories: 130
Fat: 9g
Cholesterol: 30mg
Sodium: 80mg
Carbohydrates: 13g
Dietary Fiber: 0g
Protein: 2g
These are DELICIOUS!!!My favorite cookie of all time!They do take a while to make but oh so worth it.I made a batch for a baby shower and I secretly watched people only take 1 or 2 and then go back for handfuls! They are absoloutely DELICIOUS!!!
These cookies are so delicious and addictive. I made them heart shaped for a Valentine's Day Tea Party and my friend told me that the girls loved them. I'm planning to make them again, they are just melt in your mouth delicious!
The person below who rated one star obviously didn't put the cream filling inside, as the sweetness comes from the filling! They are one of our family's all-time favorite cookies. Fussy to make, YES, but SO worth the result!!
I made the middle with almond instead of fruit flavored. These are my all time favorite cookie
This is the best cookie, I first had it at my bridal shower 31 years ago and I still love these and will make them for my daughter
Excellent recipe. These cookies are not to be eaten without the filling as the cookie itself has no sugar. It's the filling that gives it it's sweetness. Be careful......they are addictive. Also, this cookie does not freeze well so don't make more than a day or two ahead.
I have made these cookies for 40 years for holiday's and wedding's. everyone like's them.Joan
These were so good--tiny and flaky. Great for little girls' tea parties or birthday parties with the pastel icing. I put little plates of these on the decorated table along with the birthday cake for my daughter's 7th birthday. She was so thrilled.
These were AWFUL. They reminded me of the communion wafers we had as a kid in church. No flavor, except the flour. I ended up throwing them away. I wouldn't recommend. One good thing, they were easy to roll and cut out with cookie cutters.
OMG Delicious!! These cookies are so worth the extra bit of effort to prepare. So rich and buttery. Everyone that tries this cookie loves it. Make them for weddings, birthdays, Christmas and Valentine's Day!!
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Once the temperature drops a few degrees, I'm in full-on fall foods mode. Pumpkin bars, apple crisp, cranberry bread…you name it, I'm making it. However, most of it revolves around sweets. Why doesn't dinner get a fair chance?
Once the temperature drops a few degrees, I'm in full-on fall foods mode. Pumpkin bars, apple crisp, cranberry bread…you name it, I'm making it. However, most of it revolves around sweets. Why doesn't dinner get a fair chance? More ...
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Hi, I'm Becky. I'm eager to share my best ideas and favorite recipes from my 20+ years in the Land O'Lakes Test Kitchen. I'm also excited to help with any questions you have about cooking and baking with Land O Lakes® products. Some of the questions and answers will be published in our newsletter, The Measuring Cup®. Please use the short form below to ask your questions, or share your tip or memory.
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3 Reviews
My mom used to sell Pampered Chef, and makes this recipe every year. So moist, delicious, and easy! (of course) Thanks for posting it here.
Another amazing Pampered Chef recipe. Thank you for posting this! This was so easy to make & had all the fantastic flavour of a pumpkin pie w/o all the work! I used 2 cans of sweetened condensed milk (which was about 10ounces shy of what was suggested)-still turned out great. not too sweet, nice & fluffy & my guests loved it!!! no more pumpkin pie for me-this is the recipe i'll be making instead!!!
This is my favorite pumpkin pie recipe. The crust is the best!


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About this item
About this item
Forbidden longings and paranormal passions, in three stories set in the Scottish highlands. Witches, vampires and demons face their all-consuming quests-- and their primitive lusts.
"New York Times" bestselling author Hannah Howell, Victoria Dahl, and Heather Grothaus offer three unforgettable stories set in the Scottish highlands, where forbidden longings take over. . .
"The Beast Within" by Hannah Howell
Gybbon MacNachton spends his days searching for the Lost Ones--demons with the powerful MacNachton bloodline who are being hunted by those who want to destroy them. When he stumbles upon Alice Boyd, living like a wild animal in the forest, she stirs a primitive lust deep within him--a lust that can only be sated by their explosive union. . .
"Laird of Midnight" by Victoria Dahl
For fifty years, Finlay Maclain has hunted the clan of vampires who murdered his family and turned him into one of them. Now, he's at the end of his quest, sitting at a tavern, waiting for the last vampire he must kill. But the tavern's beautiful wench is distracting him beyond reason--and all he can think of is teasing her until she begs him to take her again and again. . .
"The Vampire Hunter" by Heather Grothaus
As the last surviving member of her family of witches, Beatrix Levenach must protect the townspeople from the vampires who stalk the land. But when her life is placed in danger, she receives help from Alder the White--a vampire from the clan responsible for wiping out her ancestors over a century ago. With no other choice, Beatrix accepts Alder's help. And when they discover an all-consuming passion, they must make the ultimate sacrifice for an eternal love. . .
|Contributed by:
|Howell, Hannah
|Contributed by:
|Dahl, Victoria
|Kensington Pub Corp
|Publish Date:
|Sep 2009
|Number of Pages:
|Shipping Weight (in pounds):
|Product in Inches (L x W x H):
|5.5 x 0.8 x 8.1
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So ladies and gents, we are officially house hunting! It's super exciting, but I can see that the process sometimes takes a really long time. At first I really thought it would be a simple thing, see a house that looks good online, set up an appointment to see it, or hit the open house, love it, put in an offer and done! In theory that's it, but we have run into pushy agents, deceiving photos, and some other bizarre things. We haven't even put in any offers yet! Maybe we are being typical first time home buyers, but whatever this is a super big deal … it's our first home, first mortgage, and it's a big deal so yes we are being selective.
We have picked an area we would like to live in and we've been going through Zillow, Trulia, and other sites to look at different homes within the target area. Once, we see something good (or our agent alerts us to something that might work) we either drive by and look at the outside, what the neighborhood is like and research the local schools. If we can't make it to see the neighborhood, I will use Google maps to see what the surrounding area looks like, it's very very helpful. In fact, we've crossed homes off our list because the area just didn't have the feel we are looking for. For example, main road and a huge armory directly across the street. Talk about eye-sore!
But if I've learned one thing, it's not to get overly excited about any home. In fact, I've decided not to get attached to anything until all the paperwork is signed and the keys are in my hand! But that still doesn't stop me from making Pinterest boards for potential houses with design ideas.
So ladies, do you own? If so, how was the purchase process?


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Hello! Welcome to my website! Not only do I perform a PATSY CLINE TRIBUTE SHOW for my devoted fans, but I also sing with DIANE PAUL in a duo we call GLITZANDO, performing standards and hits of the 40s, 50s & 60s. You can 'LIKE' my PATSY CLINE TRIBUTE ARTIST - CATHI RHODES page as well as our GLITZANDO page, both on Facebook!
I am honored to be singing for my 6th year on Pittsburgh's Gateway Clipper Fleet for Luncheon Cruises. ** AUGUST 21 IS SOLD OUT! **
I have been invited back to perform at selected UPMC Assisted Living Residences in the Pittsburgh area for MORE shows.
Diane & I were selected as one of the many top performers for this summer's 41st Anniversary of the Westmoreland Arts & Heritage Festival at the beautiful Twin Lakes Park. I performed my PORTRAIT OF PATSY Tribute Show (for the 6th year in a row) on July 4th on the Laurel Stage, and Diane & I performed our SENTIMENTAL JUKEBOX SHOW the same day on the Hillside Stage.
***** HOT NEWS!!!! I AM IN THE PROCESS OF PLANNING A CRUISE FOR THE SPRING OF 2016!!! We will take a bus from Greensburg & Monroeville to depart from Baltimore Harbor, cruise on Carnival's newly refurbished ship, the PRIDE, down to GRAND TURK, HALF MOON CAY and FREEPORT, BAHAMAS and back up the coast. If you are interested in joining us, email me at email@example.com and I will get all the details to you ASAP! I am hoping you will consider joining us on this amazing cruise. You can't beat the price for everything you'll need: Hotel room, fine dining and casual foods, excellent room service, entertainment, swimming, CASINO, etc. And there are numerous activities available for every member of your family! TAXES AND GRATUITIES ARE INCLUDED! (The ONLY thing not included are alcoholic beverages.) Think about what you spent the last time you took your family on vacation. I highly doubt it was as low as this cruise will cost you! REALIZE THE VALUE OF THIS CRUISE! And we are going to enjoy a private island beach party too! There ARE still rooms available, however, it's best if you reserve your room NOW. Just $50 holds your reservation! As I have been saying, "DON'T DALLY! CALL SALLY! 724-468-0373." (SEE THE FLIER ON MY PHOTO PAGE!)
Thanks, to all for such kind comments. You're the best! VISIT ME ON FACEBOOK - at "Patsy Cline Tribute Artist - Cathi Rhodes" and on "Glitzando." PLEASE CHECK OUT THE 'NEWS' PAGE on this website , as I want to keep this Homepage available for CURRENT updates. I also want to remind you to check out the 'Performances' page. Everyone is always asking WHEN and WHERE I'll be singing for THE GENERAL PUBLIC, so I hope you will be able to make it to hear me perform.
If you are looking for quality entertainment -- you just found it! Call me and we can make arrangements for YOUR special event! Thank you for coming out and joining the fun!! God Bless and enjoy your day!


text: The Brian Lehrer Show Edit this Published in The Brian Lehrer Show Tracing "25 Random Things" Listen 9 min Queue Download this audio Want to embed this player? Use the code below. More info. <iframe frameborder="0" scrolling="no" height="130" width="100%" src="https://www.wnyc.org/widgets/ondemand_player/wnyc/#file=/audio/json/29770/&share=1"></iframe> Share on Facebook Share on Twitter Email a Friend Chris Wilson, assistant editor of Slate Magazine, looks at the evolution of the Facebook phenomenon "25 Random Things About Me."


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Pittsburgh Penguins 1991 CBC Tribute
This tribute to the pittsburgh penguins was played following their 8-0 victory over the Minnesota Stars in 1991. Lemieux's and Pittsburgh first Stanley Cup.
This tribute to the pittsburgh penguins was played following their 8-0 victory over the Minnesota Stars in 1991. Lemieux's and Pittsburgh first Stanley Cup.
Penguins hockey players went to Pittsburgh's Hill District and delivered frozen turkeys as part of a food drive before Thanksgiving.
Sidney Crosby Delivers Season Tickets To Penguins Fan
An epic match-up of the game's best young guns. WHo will prevail? You tell me.
decorate your walls with this brand new poster easy to frame and makes a great gift too ships quickly and safely in a sturdy protective tube measures 22.00 by 34.00 inches Product DescriptionPittsburgh Penguins (2009 Champions, Sidney Crosby) Sports Poster Print – 22×34… More >> Pittsburgh Penguins Sports Poster Print – 22×34
John Barbaro, the arena voice of the Pens is profiled. [From FSN © 2007. ALL COPYRIGHTS ARE PROPERTY OF THEIR RESPECTIVE OWNERS.]
The Penguins give the jerseys off their backs for the best fans in hockey
From the draft of Malkin, to the signing of Satan. The last few years in Penguins hockey.
Product DescriptionSupport your team on and off the ice with this Reebok® Pittsburgh Penguins Evgeni Malkin women's premier third jersey! This high-quality premier polyester jersey is slightly fitted to complement your feminine shape and designed with official player and team graphics screen-printed on the chest, back, sleeves and shoulders…. More >> Reebok Pittsburgh Penguins Evgeni…
Hockey Night in Canada show a video montage of most of the infractions that were not called against the Penguins during the game


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text: Can The Health Benefits Of Papaya Help You? September 9, 2015October 6, 2015 katherine What are the unique health benefits of papaya? Papaya, or PawPaw, is full of vitamin C and an excellent source of beta carotene as well. Half a papaya will give you all of the vitamin C that you need that day. The only problem with papaya is that our bodies seem to absorb it a […]... Read More


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Multimedia Exhibit For Dogs
Event looks at the man's best friend from a distinguished perspective.
Recently, the gallery Matilha Cultural ("Cultural Pack of Dogs", in a free translation), in São Paulo, hosted a series of exhibitions and activities aimed at every man's best friend. The práCachorro ("forDog") show, which received animals and their owners, had various attractions such as cinema, visual art, a space dedicated to pet adoption and a special menu for dogs.
Interactive multimedia installations provided special moments between humans and dogs. Lectures, discussions and support for the adoption of animals were part of the program, which lasted one month, with the support of Pedrigree's "Adotar é tudo de bom ("Adoption is just great") campaign.
Also featured at the event were lectures about dog health and nutrition, adoption and responsible ownership, animal care and rights organizations, a photo exhibit of dogs and their owners, as well as a lounge area where dogs could relax or play freely within the pack.
In the gallery, the photo exhibit by Paola Vianna revealed the dog's view of the world. Panels illustrated with the story Nosso Amigo Vira-lata ("Our Friendly Stray Dog"), written by Evaristo Eduardo de Miranda for National Geographic Brazil (March 2010), detailed the historical importance of dogs in our lives.
A broad musical program with hip hop, soul, funk, world music and reggae completed the exhibition dedicated to canine celebration.
PRÁ CACHORRO from Matilha Cultural on Vimeo.
Contributed by Nara Bianconi
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We are glad to bring you to Audiko, where you can search for ringtones for free for your mobile. Today we have a ringtone of "Black Angel" by The Cult as well as 179 other ringtones by The Cult. "Black Angel" is a gorgeous track from the "Love" album that was produced by The Cult at their label together with other 13 tracks. You can listen to all 4 The Cult – "Black Angel" ringtone variations available here and download it for free right here. You can also take a glance at other 11 albums produced by The Cult for all time. "Black Angel" is referred to Rock, and we can offer you a huge amount of Rock albums absolutely for free. And finally, we have collected lyrics for this track and you can view it here as well. So feel free to browse, listen, download ringtones at Audiko.net. Make your phone sound cool!
The Cult Black Angel Ringtone
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StartUpHire is the place to find Senior Marketing Manager jobs. Review the list of Senior Marketing Manager career opportunities to the left. These Senior Marketing Manager jobs represent some of the most rewarding careers available.
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Exciting position at a Health and Wellness startup in Silicon Valley.10/1/2015 Mountain View, CA, CA Save This Job
As a Senior Marketing Manager, User Acquisition on the Kongregate User Acquisition team, your mission is to maximize the growth of our mobile games. Your primary responsibility is to take a comprehensive approach to growth using every tool at your disposal with the explicit8/9/2015 San Francisco, CA Save This Job
Job Description Job Description The PatientsLikeMe Marketing team is responsible for creating the strategy, positioning and execution plan for the marketing of PatientsLikeMe to both consumers and industry partners. As the Senior Marketing Manager, you will execute marketing8/25/2015 Cambridge, MA Save This Job
at AdRoll San Francisco AdRoll, headquartered in downtown San Francisco with additional offices in New York, Dublin, London, Sydney, and Tokyo, is a performance marketing platform that helps brands of all sizes run highly targeted, data-driven digital marketing campaigns. Named10/6/2015 San Francisco, CA Save This Job
Marketing Sunnyvale, CA, United States We are seeking a high caliber marketing professional with experience and aspiration to grow in the fast-paced digital marketing space to join as the Marketing Manager for our new ?Xclaim? product brand. This is a unique opportunity to9/2/2015 Sunnyvale, CA Save This Job
Description: Luidia is looking for a Sr. Marketing Manager who can develop and execute various components of a Marketing Strategy within the company. This is a critical role requiring an experienced marketing professional with background and exposure to product marketing, PR,9/8/2015 Belmont, CA Save This Job
The Position The Marketing Manager, Customer Acquisition is responsible for subscriber/customer acquisition for our Retail Wi-Fi and Broadband (Military) product lines. This role is ideal for the high-performing data-driven marketer with a passion for the science and art of8/11/2015 Los Angeles, CA Save This Job
Location US-Austin Posted Date 8/26/2015 Options Overview Who you are: Are you a saavy marketer who loves to help drive hyper growth at top accounts? Do you embrace the challenge of crafting marketing plans to turn key customers into brand fanatics? Have you high-fived your9/10/2015 Austin, TX Save This Job
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San Mateo, CA, United States Full-Time Edmodo is looking for a creative, energetic, entrepreneurial candidate for a Senior Manager for Marketing, reporting to the General Manager, Institutions. The ideal candidate will have the following qualities: Creative &10/1/2015 San Mateo, CA Save This Job
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Location:Renton, WA Job Code:352 # of openings:1 Description CAREER OPPORTUNITY Want to play a principle role in driving the cloud computing revolution? Consider taking on this exciting and challenging role at Odin. We are a private, profitable and rapidly growing global9/24/2015 Renton, WA Save This Job
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Zendesk is looking for a Senior Field Marketing Manager, based in the San Francisco/Bay Area. As the Senior Field Marketing Manager at Zendesk, you are responsible for developing and managing field-based demand generation programs and campaigns to build awareness, drive and10/6/2015 San Francisco, CA Save This Job


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Brooklyn Nets v Houston Rockets
James Harden of the Houston Rockets goes to the basket against Gerald... Fotografia de notíciasBasquetebol,Basquetebol - NBA Pro,Brooklyn Nets - Equipa de basquetebol,Desporto,EUA,Estados da Costa do Golfo,Gerald Wallace,Houston,Houston Rockets - Equipa de basquetebol,James Harden,Texas,Toyota Center,VerticalPhotographer Bill BaptistCollection: National Basketball Association 2013 NBAEHOUSTON, TX - JANUARY 26: James Harden #13 of the Houston Rockets goes to the basket against Gerald Wallace #45 of the Brooklyn Nets on January 26, 2013 at the Toyota Center in Houston, Texas. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and or using this photograph, User is consenting to the terms and conditions of the Getty Images License Agreement. Mandatory Copyright Notice: Copyright 2013 NBAE (Photo by Bill Baptist/NBAE via Getty Images)


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Over the years fans have watched Lauren Conrad grow up as the main protagonist on [url id="http://www.mtv.com/shows/the_hills/season_6/series.jhtml"]"The Hills."[/url] We were there when she graduated from high school on "Laguna Beach" and when she decided to move to L.A. to study fashion in college.
We saw her first love with Jason Wahler and her first heartbreak . "I don't want you out of my life, but I can't be normal with you," she later told Wahler when they decided to end their relationship.
Fans also got to see the sweet beginning and bitter end to the friendship with Heidi Montag . "We don't really have a relationship," LC later said about Heidi. "We're friendly with each other. I don't have a problem with her, but, you know, we're not great friends."
Yet when Lauren decided to leave the show in 2009, viewers couldn't imagine "The Hills" without her — until it was announced that a familiar face and her former "Laguna Beach" frenemy was moving back in. Kristin Cavallari immediately caused a splash in "The Hills" reminding us all why we loved to hate her.
"It's almost like going home. It's really comfortable," Cavallari said of joining the show in its fifth season. "I know what to expect going into it, so I think I'm just more excited than anything."
So with "The Hills" coming to a close this season, Cavallari is now, once again, center stage, which means we're sure to see a whole lot of drama. But we'll still keep our fingers crossed that Lauren makes a return , even if she's hinted to the fact that she's already moved on.
"I'm not planning on it," she said last year. "I'm happy with the time I walked away, and I think that from now on, they're going to pick it up and do a great show. But I think that my time on it is done."
What are some of your favorite "Hills" moments? Let us know in the comments below!
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text:
Page 1
Atherogenic diet-induced hepatitis is partially dependent on
murine TLR4
Moreshwar S. Desai,*,†M. Michele Mariscalco,*,†Ahmad Tawil,†J. G. Vallejo,‡
and C. W. Smith†,1
*Pediatric Critical Care Medicine,†Section of Leukocyte Biology, Children's Nutrition Research Center, and
‡Infectious Diseases and Winters Center For Heart Failure Research, Department of Pediatrics, Baylor College of
Medicine, Houston, Texas, USA
such as the Paigen diet have been used to study
atherogenesis, lithogenesis, and proinflammatory
microvascular changes induced by nutritional hy-
percholesterolemia. Although these diets lead to
chronic hepatic inflammation and fibrosis, the
early inflammatory changes have been poorly char-
acterized. TLR4, a known receptor for LPS, is also
a receptor for a variety of endogenous ligands and
has been implicated in atheroma formation. Here,
we specifically examined the early inflammatory
response of the liver to the atherogenic (ATH) diet
and the possible contribution of TLR4. Animals fed
the high-cholesterol/cholate diet for 3 weeks devel-
oped a significant, predominantly mononuclear
leukocyte infiltration in the liver, hepatic steatosis,
elevated hepatic expression of MCP-1, RANTES,
and MIP-2, and increased serum levels of liver
enzymes. In TLR4-deleted animals, there was a
30% attenuation in the serum alanine transami-
nase levels and a 50% reduction in the leukocyte
infiltration with a fourfold reduction in chemokine
expression. In contrast, hepatic steatosis did not
differ from wild-type controls. TLR2 deletion had
no effect on diet-induced hepatitis but increased
the amount of steatosis. We conclude that the early
inflammatory liver injury but not hepatic lipid load-
ing induced by the ATH diet in mice is mediated in
part by TLR4. J. Leukoc. Biol. 83: 1336–1344;
Diets high in cholesterol and cholate
Key Words: cholesterol/cholate diet ? inflammation ? TLR2 ? che-
A diet high in cholesterol (1.25%), fat (15%), and cholate
(0.5%) described by Paigen and co-workers [1] has been used
to induce proinflammatory changes in the microvasculature [2],
increase production of reactive oxygen species [3], elevate
expression of adhesion molecules in the endothelial cells [4],
enhance adherence and emigration of granulocytes [5], en-
hance T cell-mediated release of the proinflammatory cytokine
IFN-? [6], and increase the platelet–leukocyte interaction [7].
In addition, this diet has been shown to induce nutritional
hypercholesterolemia, atherogenesis [8], and cholesterol gall-
stone disease in inbred mouse strains [9, 10]. Three weeks
diet-fed mice demonstrate increased plasma cholesterol, low-
density lipoprotein (LDL)/very LDL cholesterol, nonesterified
cholesterol, and reduced high-density lipoprotein cholesterol
compared with chow-fed animals [11].
In rats, this diet results in lipid-laden hepatic parenchymal
cells and Kupffer cells [12], mild hepatic fibrosis [13], and
oxidative hepatocellular injury [14, 15]. At the molecular level,
this diet at 5–15 weeks has been shown to activate hepatic
NF-?B and induce mRNA for the mouse homologue of MCP-1
(CCL2), colony-stimulating factors, heme oxygenase, and mem-
bers of the serum amyloid A family in the liver. A correlation
was also reported between hepatic inflammatory gene induction
and susceptibility to fatty-streak development [16]. However,
there has been little characterization of the early stages of
hepatic inflammation. The liver plays a critical role in the
metabolism of cholesterol [17, 18] and bile acids [19], which
influence atherogenesis [20, 21] and lithogenesis [22].
TLR4, a transmembrane protein, is known to activate proin-
flammatory pathways in response to LPS, leading to production
of inflammatory cytokines in various tissues including the liver
[23]. It is also clear that endogenous ligands exist for TLR4. It
is thought that these endogenous substances are responsible for
instituting the host innate immune response during noninfec-
tious stress events [24, 25]. It has also been shown that dietary
hypercholesterolemia sensitizes the liver to endotoxemia, and
it has been postulated that overexpression of hepatic TLR4
may contribute to the observed phenomenon [26]. As such,
TLR4 has been implicated in the process of atheroma gener-
ation [27–30]. Recently, there has also been literature support-
ing a role for TLR2 in atherogenesis [31, 32]. As liver-medi-
ated processes along with hypercholesterolemia are critical for
atheroma generation, we assessed the possible contributions of
TLR4 and TLR2 to the early liver injury associated with
diet-induced hypercholesterolemia.
1Correspondence: Section of Leukocyte Biology, Department of Pediatrics,
Baylor College of Medicine, 1100 Bates Ave., Suite 6014, Houston, TX
77030-2600, USA. E-mail: email@example.com
Received June 11, 2007; revised January 17, 2008; accepted February 5,
doi: 10.1189/jlb.0607390
1336 Journal of Leukocyte Biology
Volume 83, June 2008
0741-5400/08/0083-1336 © Society for Leukocyte Biology
Page 2
Male mice, 8–10 weeks old, 25–28 g, were used for all of the experiments.
C57BL/6J (strain #664) mice were used to establish the model. For evaluating
the role of TLR4, C57BL/10SNJ-TLR4-deleted (TLR4del) mice were raised in
our animal facility from homozygous mating (strain #3752). C57BL/10SnJ
(strain #666) were the controls for the TLR4delstrain (Jackson Laboratories,
Bar Harbor, ME, USA). For the TLR2 experiments, heterozygous, TLR2-
deficient mice were backcrossed six generations on a C57BL/6NHsd (Harlan
Sprague Dawley Inc., Indianapolis, IN, USA) background. After genotyping
using the appropriate primers, 8- to 10-week-old male, TLR2delmice were
used with age- and gender-matched C57BL/6NHsd controls (Harlan Sprague
Dawley Inc.). Mice were housed in a room with a 12-h light cycle and had free
access to food and water. The Institutional Animal Care and Use Committee at
Baylor College of Medicine (Houston, TX, USA) approved all protocols.
Mice were fed an atherogenic (ATH) "Paigen" diet [1.25% (w/w) cholesterol,
0.5% (w/w) cholic acid (CA), and 16% (w/w) fats in the form of soybean oil,
cocoa butter, and coconut oil] or isocaloric (ISO) control chow (0.3% w/w
cholesterol, no CA, and 5% w/w fats) for 3 weeks for each experiment. Both
diets were obtained from Research Diets Inc. (New Brunswick, NJ, USA). They
were irradiated and stored per recommendations from the manufacturer. Feeds
were weighed and changed twice a week, and animals were weighed each week
to monitor weight gain. Rodent chow supplemented with 0.5% CA was spe-
cially formulated and ordered separately from Harlan (Harlan Teklad, Madi-
son, WI, USA) for separate sets of experiments to evaluate effects of dietary
Experimental groups
Four sets of experiments were performed. 1) To study the effects of diet on liver
inflammation, C57BL/6J mice were assigned to an ATH diet or ISO chow
group. After 3 weeks of diet, animals were anesthetized, blood was collected
from vena cava, and the animals were killed. Liver was obtained for further
analysis. Animals were analyzed in two groups: ISO (n?4) and ATH (n?5). 2)
To study the effects of cholate supplementation on liver inflammation,
C57BL/6J mice were assigned to cholate supplemented or standard rodent
chow. After 3 weeks of diet, animals were anesthetized, blood was collected
from vena cava, and the animals were killed. Liver was obtained for further
analysis. Animals were analyzed in two groups: chow (n?4) and CA-supple-
mented chow (n?5). 3) To assess the role of TLR4, TLR4del(C57BL/10SNJ)
mice and their wild-type (WT) controls (C57BL/10SnJ) were randomly as-
signed to an ATH diet or ISO chow for a duration of 3 weeks. Mice were fasted
for 6 h prior to sacrifice. Results were analyzed according to their respective
groups: WT ? ISO (n?5); WT ? ATH (n?6); TLR4del? ISO (n?5); and
TLR4del? ATH (n?6). 4) For TLR2 experiments, TLR2delmice and their WT
controls (C57BL/6NHsd) were randomly assigned to ATH or ISO chow for a
duration of 3 weeks. Mice were fasted for 6 h prior to sacrifice. Results were
analyzed according to their respective groups: WT ? ISO (n?4); WT ? ATH
(n?6); TLR2del? ISO (n?4); and TLR2del? ATH (n?6).
Serum alanine transaminase (ALT) activity and
cholesterol levels
Nonhemolyzed serum from blood samples collected from the vena cava was
used for determination of ALT activity and serum cholesterol levels using a
kinetic spectrophotometric assay (Thermo Electron, Louisville, CO, USA).
Portal venous endotoxin measurement
C57BL/6 mice (n?12) were separately fed an ATH diet (n?8) or ISO chow
(n?4) for 3 weeks. Blood was collected from the portal vein prior to sacrifice.
Platelet-rich plasma fraction was isolated from pooled, heparinized blood
samples, which were prepared as described previously [33], and endotoxin was
detected using a kinetic chromogenic assay using the kinetic QCL kit (Cam-
brex, Walkersville, MD, USA).
Sections of liver preserved in formalin-free zinc fixative (BD PharMingen, San
Diego, CA, USA) were embedded in paraffin, sectioned, and stained with H&E.
To demonstrate hepatic lipid accumulation, additional sections of liver were
embedded in OCT and frozen at –80°C and subsequently stained with Oil
Red-O (Sigma Chemical Co., St. Louis MO, USA).
Immunohistochemistry was performed on 4 ?m-thick sections of paraffin-
embedded liver tissue. The following were used: Pan-leukocyte marker, anti-
mouse CD45 (leukocyte common antigen, Ly-5, clone 30-F11, BD PharMin-
gen) at 1:20 dilution; rat anti-mouse F4/80 for monocyte/macrophages includ-
ing Kupffer cells (Serotec, Raleigh, NC, USA) at a dilution of 1:50; and rat
anti-mouse neutrophil antibody (Serotec) at a dilution of 1:50. Biotinylated,
HRP-tagged rabbit anti-rat antibody (Vectastain ABC kit, Vector Laboratories,
Burlingame, CA, USA) was used as a secondary antibody at a dilution of 1:200.
Brown staining of target cells was obtained by using a working solution of
3,3?-diaminobenzidene substrate (Vector Laboratories). Liver was counter-
stained with Gill's hematoxylin (Fisher Diagnostics, Middletown, VA, USA)
and visualized directly under 10? and 60? magnification under light micro-
scope (Leitz, Germany) and evaluated using a SPOT camera (Diagnostics
Instruments Inc., Sterling Heights, MI, USA). The contribution of platelets was
evaluated by staining paraffin-embedded liver sections (4 ?-thick) with anti-
mouse, CD41-PE labeled antibody (BD PharMingen; 1:100; clone MWreg30)
and mounting in Airvol (Celenase, Ltd., Dallas, TX, USA) containing 1 ?mol/L
4?,6-diamidino-2-phenylindole (DAPI; Sigma Chemical Co.). Slides were
counterstained with DAPI to assess nuclear morphology. Sections were exam-
ined by immunofluorescence (Delta Vision, Applied Precision, Issaquah, WA,
Quantification of inflammatory cells
Ten high-power fields (60?) per 4 ?m-thick section of stained liver per animal
were randomly selected (http://www.randomizer.org). The level of inflammation
was assessed morphometrically as the volume percent of hepatic parenchyma
comprised of the inflammatory cells (percent volume) per high-power field
using an unbiased, stereological technique described by Howard and Reed
[34]. To derive an unbiased estimate of the volume fraction (percent surface
area per 4 ?m-thick section) of hepatic tissue composed of the cell of interest,
a cycloid grid (ref. [34], C-1, Page 210, Appendix B) was randomly positioned
on the field, and the number of points of the grid hitting the nucleus of the
stained inflammatory cell divided by the number of points hitting the hepatic
parenchyma (including the sinusoids) gave an unbiased estimate of volume
fraction (volume percent). Volume fraction (percent volume) ? P(Y)/P (ref).
Where P(Y) is the number of points on the grid hitting the nuclei of interest,
and P (ref) is the total number of points hitting the hepatic parenchyma
(including the sinusoids) per high-power field. Care was taken not to count the
cells in the areas of the central vein, the portal vein, and the hepatic artery.
Hepatic lipid extraction and analysis
The Bligh-Dyer lipid extraction method was used to quantify the amount of
lipids in the liver tissue [35]. Briefly, 0.4 g liver in 1 ml PBS was mechanically
disrupted in a sonicator, and then 0.5 ml chloroform and 1 ml methanol (1:20)
were added and vortexed vigorously for 1 min. Chloroform (0.5 ml) was added
again and vortexed for 1 min. The mixture was centrifuged at 3000 rpm 5 min
to separate the phases. The bottom (organic lipid) phase was transferred to
preweighed glass tubes and dried in a hood for 48 h. The dried lipid layer was
weighed and was reported in mg of lipids per gram of liver tissue. Methyl esters
of the total lipid fraction were prepared with boron trifluoride-methanol as
described earlier [36] and quantified by gas liquid chromatography (Hewlett-
Packard 5890 gas chromatograph) on a DB-225 capillary column (J & W
Scientific, Folsom, CA, USA). Fatty acids were identified by comparison with
the retention times of fatty acid methyl ester standards.
IFN-? and IL-6 levels in liver tissue lysate
Quantitative assessment of liver tissue IFN-? and IL-6 content was done using
ELISA kits specific for tissue lysate (Ray-Biotech, Inc., Norcross, GA, USA).
Liver tissue lysate was prepared per kit instructions. Protein levels in the
Desai et al.
TLR4 in liver injury with atherogenic diet1337
Page 3
tissue extracts were measured using the Micro-bicinchoninic acid protein
assay kit (Pierce Chemical Co., Rockford, IL, USA). Results were reported in
picograms per gram of protein loaded.
Quantitative real-time PCR
Snap-frozen liver samples were homogenized in QIAzol lysis reagent, and total
RNA was isolated with the RNeasy Mini kit (Qiagen, Valencia, CA, USA).
Quantity and quality of the extracted RNA were verified using a Nanodrop-
1000 spectrophotometer (Nanodrop Technology, Wilmington, DE, USA). Syn-
thesis of cDNA was performed with the TaqMan reverse transcription kit
(Applied Biosystems, Foster City, CA, USA) using 2 ?g total RNA and random
hexamer primers per the manufacturer's recommendations. For the amplifica-
tion of ICAM-1 and MCP-1, 5 ?l cDNA was added to a corresponding 20?
TaqMan MGB probe primer set for each message, multiplexed with primers for
GAPDH and 2? TaqMan Universal PCR master mix (Applied Biosystems).
PCR was performed in a 7500 real-time PCR system (Applied Biosystems)
using the manufacturer's suggested thermal settings: one cycle of 2 min at
50°C, followed by 10 min at 95°C and 40 cycles of 15 s at 95°C and 60 s at
60°C. Relative mRNA expression was calculated as the ? comparative thresh-
old (Ct) method. GAPDH was used as internal control.
RNase protection assay
RANTES (CCL5), eotaxin (CCL11), lymphotactin (XCL1), MIP-2 (CXCL2),
IFN-?-induced protein (IP-10; CXCL10), and T cell activation gene 3 (TCA-3;
CCL1) gene expression was determined by the use of a custom-designed
multiprobe RNase protection assay system (mck-5, RiboQuant, PharMingen),
which was used according to the manufacturer's protocol. Total RNA was
extracted from snap-frozen livers as described above, and probe synthesis,
RNA hybridization, RNase treatment, and specific probe purification, electro-
phoresis, and imaging were done as described previously [37]. Signals were
quantified by use of software (Image QuaNT, Molecular Dynamics, Sunnyvale,
CA, USA) and were normalized to L32.
Statistical analysis
Data are presented as means ? SEM. Data were analyzed using ANOVA, and
differences between groups were identified using Neuman-Keuls post hoc,
unless specified. All statistical calculations were done using the PRISM 3.0
software program (Graphpad Prism, San Diego, CA, USA). P ? 0.05 was
selected as the level of significance.
ATH diet induces hypercholesterolemia, a
predominant mononuclear cell infiltrate, liver
injury, and hepatic steatosis
Serum cholesterol levels were increased significantly in the
C57BL6/J mice fed the ATH diet for 3 weeks compared with
animals fed ISO chow (180.5?14.85 mg/dl vs. 128?5.5 mg/dl
in C57BL6/J; P?0.05). Leukocyte infiltrates were evident in
the periportal area in the animals on the ATH diet, compared
with the animals on an ISO diet (Fig. 1, A–B). The majority of
these cells in the liver of animals on the ATH diet was F4/80?,
consistent with increases in monocyte/macrophage and Kupffer
cells. Neutrophil influx was also seen (Fig. 1E). Oil Red-O
staining revealed microvesicular steatosis with lipid deposition
in the hepatocytes (Fig. 1, C–D). Serum ALT (Fig. 1E) levels
were increased in C57BL6/J after 3 weeks on the ATH diet,
consistent with diet-mediated liver injury. These results were
reproduced in the C57BL10/SnJ mice, which were separately
used as WT controls for the TLR4delanimals (Figs. 2a and 3).
Cholate added to the chow diet alone for 3 weeks also
induced mild liver elevation of the liver enzymes (serum ALT
levels: 147?33 ?L vs. 36?3 ?L; P?0.028; unpaired t-test)
and a modest increase in leukocytes (percent volume of
CD45? cells: 1.4?0.25 vs. 0.4?0.05; P?0.024) when com-
pared with standard rodent chow-fed C57BL/6J animals. It
should be noted that this amount of injury and inflammation
alone was ?50% of that seen in animals eating the ATH diet
for 3 weeks (Fig. 1E). However, unlike those animals, animals
whose diet was supplemented with cholate alone had no evi-
dence of hepatic lipid loading [lipid levels: 17.50?2.44 mg/g
liver in cholate-fed vs. 21.65?1.144 mg/g liver in chow-fed;
P?not significant (ns)].
Fig. 1. Effect of an ATH diet on liver injury in C57BL6/J mice. Repre-
sentative photomicrographs of livers of mice fed ISO chow (A) and an ATH
diet (B) stained with H&E; (arrow) peri-portal mononuclear cell infiltrates.
(C and D) Oil Red-O staining. Red staining (D) indicates intracellular lipid
deposition after 3 weeks of an ATH diet (10? original magnification;
original scale bar?100 ?). (E; Left) Morphometric quantification of hepatic
parenchyma stained by immunohistochemistry for monocytes/macro-
phages/Kupffer cells (F4/80?) and polymorphonuclear cells (PMN) cells.
Values denote volume fraction (% Volume) of cells of interest per hepatic
parenchyma per high-power field (60? original). A total of 10 high-power
fields counted per liver per mouse. (Right) ALT levels in the serum after feeding an ATH diet or ISO chow. Results are means ? SEM (n?4?5 per group); *, P
? 0.002, versus ISO-fed groups using unpaired two-tailed t-test.
1338 Journal of Leukocyte Biology
Volume 83, June 2008
Page 4
There was no evidence of portal venous endotoxin in
C57BL/6J mice, irrespective of their diet, as measured by
kinetic chromogenic assay (data not shown).
TLR4 deletion attenuates the inflammatory
response and hepatic injury in mice on an ATH
TLR4delanimals fed the ATH diet had attenuation of the serum
ALT levels by ?30% compared with their WT C57BL10/SnJ
controls (186?22 ?L vs. 247?18 ?L; P?0.05; Fig. 2a). There
were also 50% fewer CD45? cells (Fig. 2b), F4/80? cells, and
neutrophils in the ATH diet-fed TLR4delanimals compared
with their ATH diet-fed WT controls (Fig. 3). TLR4delanimals
fed an ATH diet showed a small but significant (fourfold)
increase in the F4/80? cells compared with the ISO-fed
groups. The number of CD45? cells and polymorphonuclear
cells in the TLR4delmice fed an ATH diet was also increased
(threefold) compared with the ISO chow-fed groups but did not
reach statistical significance (Figs. 2b and 3).
ATH-fed mice showed significantly more platelets in their
liver (mostly in the sinusoidal spaces) than the ISO-fed mice
(percent volume CD41? cells: 6.1%?0.9 vs. 12.9 ?0.4;
P?0.02 by t-test). There was, however, a relative paucity of
platelets in the inflammatory foci in the livers of the WT
ATH-fed animals (Fig. 4). The deletion of TLR4 did not
appear to change the amount of platelets compared with WT
animals (percent volume CD41? cells: 12.70%?0.3 vs. 12.9
?0.4; P?ns).
There was no increase in hepatic IL-6 and IFN-? protein
expression in control or TLR4delmice, irrespective of ATH or
chow diet (data not shown).
TLR4 deletion-attenuated expression of MCP-1,
RANTES, and MIP-2
Hepatic mRNA levels for MCP-1 (CCL2), ICAM-1 (CD54),
RANTES (CCL5), MIP-2 (CXCL2), lymphotactin (XCL1),
eotaxin (CCL11), TCA-3 (CCL1), and IP-10 (CXCL10) were
increased in the livers of WT (C57BL10/SnJ) mice after feed-
Fig. 2. Effect of an ATH diet on control
and TLR4delanimals. (a) Serum ALT levels
measured in the C57BL/10SnJ (WT) and
TLR4delmice at the end of 3 weeks of
feeding an ATH diet or ISO chow. Results
are means ? SEM (n?5–6 per group); *,
P ? 0.05, versus TLR4del? ATH; #, P ?
0.01, versus ISO-fed TLR4deland WT an-
imals. (b) Representative photomicrographs
of livers of WT and TLR4delmice stained
for CD45-positive cells. (b, B) Arrow shows
CD45 ? brown-stained cells. (b, A) WT ?
ISO chow; (b, B) WT ? ATH chow; (b, C)
TLR4del? ISO chow; (b, D) TLR4del?
ATH chow. Note higher number of brown-
stained cells in b, B compared with b, D
(60? original magnification; original scale
bar?50 ?). (Right) Morphometric quantifi-
cation of hepatic parenchyma stained by
immunohistochemistry for all leukocytes
(CD45? cells). Values denote volume frac-
tion (% Vol) of cells of interest per hepatic
parenchyma per high-power field (60? original). A total of 10 high-power fields counted per liver per mouse; *, P ? 0.05, versus all groups. (c) Representative
photomicrographs of frozen sections of livers of WT (c, A and B) and TLR4delmice (c, C and D) stained with Oil Red-O. Red staining (c, B and D) indicates
intracellular lipid deposition after 3 weeks of an ATH diet (original magnification, 10?; original scale bar?100 ?). (Right) Quantification of the lipid content
of the liver in mg of lipid per gram of liver. Results are mean ? SEM (n?4–6); *, P ? 0.01, versus respective ISO-fed animals.
Desai et al.
TLR4 in liver injury with atherogenic diet1339
Page 5
ing the ATH diet (Figs. 3 and 5); ribonuclease protection
assay (RPA) analysis of WT and TLR4delISO chow-fed ani-
mals revealed no detectable bands (data not shown). Livers of
TLR4delmice had significantly less mRNA for MCP-1 (Fig. 3),
RANTES, and MIP-2 (Fig. 5).
Expression of ICAM-1 (Fig. 3), lymphotactin, eotaxin, IP-10,
and TCA-3 was similar between the two diet-fed groups, irre-
spective of TLR4 deletion (data not shown).
TLR4 deletion did not affect the hepatic lipid
loading, serum cholesterol levels, or the fatty
acid profile of diet-fed mice
Serum cholesterol levels (200?10 mg/dl vs. 196?13 mg/dl;
P?ns), hepatic lipid content as assessed by Oil Red-O
staining, and lipid extraction by the Bligh-Dyer method
were similar in the WT and TLR4delgroups fed the ATH diet
(Fig. 2c).
As nutritional saturated fatty acids, especially lauric acid
(C12:0), has been implicated as a ligand for TLR4 signaling
[38, 39], and the Paigen diet has a high content of lauric acid
and saturated fats (information from Research Diets Inc.), we
analyzed the fatty acid methyl esters from lipid extracts in each
group (n?5–6) by gas chromatography. We found statistically
higher content of lauric acid in the livers of mice fed the ATH
diet, irrespective of the presence or absence of TLR4 (Fig. 6)
The hepatic concentrations of palmitoleic acid (C16:1), oleic
acid (C18:1), linoleic acid (C18:2,n-6), ?-linolenic acid (C18:
3,n-3), and ?-linolenic acid (C18:3,n-6) were significantly
higher in ATH diet-fed livers than their chow-fed counterparts
(Fig. 6). The hepatic contents of myristic acid (C14:0), palmitic
(C16:0), stearic (C18:0), arachidic (C20:0), behenic (C22:0),
arachidonic (C20:4), eicosapentanoic, and docasahexaenoic
acids were similar among all groups, irrespective of diet or
TLR4 presence (data not shown). Except for linoleic (C18:2,n-
6), which was found to be higher in TLR4delATH-fed mice, the
fatty acid profile of the WT and the TLR4delmice was similar
within their diet groups (Fig. 6).
TLR2 deletion did not attenuate the inflammatory
response or liver injury in ATH diet-fed animals
The TLR2delmice and their WT controls, C57BL/6NHsd (Har-
lan strain), showed evidence of liver injury (ALT), inflamma-
tion (CD45? cells), hepatic steatosis, and hypercholesterol-
emia when compared with their chow-fed counterparts (Fig.
7). There was, however, no statistical difference among the
serum cholesterol levels (243.6?14.8 mg/dl vs. 225.3?17.8
mg/dl; P?ns), ALT levels, and the volume percent of hepatic
CD45? cells between the WT and the TLR2del, diet-fed groups
(Fig. 7). On Bligh-Dyer analysis, livers of diet-fed, TLR2del
mice showed a significantly higher lipid content as compared
with the diet-fed WT mice (Fig. 7).
In this paper, we demonstrate that microvesicular steatohepa-
titis occurs within weeks of feeding Paigen's ATH diet. There
is a five- to sevenfold increase in the percent of the hepatic
parenchyma of infiltrating neutrophils and over a tenfold in-
crease in the F4/80? cells in those animals fed a high-
cholesterol/cholate diet. In addition, we show that the leuko-
Fig. 3. Quantification of monocyte/macrophages, neutrophils, and quantita-
tive PCR for expression of MCP-1 and ICAM in livers of TLR4deland their WT
controls. Using immunohistochemistry and morphometric analysis, the volume
of the hepatic parenchyma, which stained for monocytes/macrophages/Kupffer
cells (F4/80?) and neutrophils, was quantified. Values denote volume fraction
(% vol) of cells of interest per hepatic parenchyma per high-power field (60?
original). A total of 10 high-power fields counted per liver per mouse. Results
are mean ? SEM (n?4–6). (Right) Bar graphs show quantitative real-time PCR
results from RNA extracted from snap-frozen livers, using probes for ICAM
and MCP-1. Relative mRNA expression was calculated by the ? Ct method
with GAPDH as endogenous control. Results are means ? SEM (n?5–6); *,
P ? 0.05, versus all groups; #, P ? 0.05, versus ISO-fed groups; ?, P ? 0.05,
versus WT ? ISO group.
Fig. 4. Platelet staining by immunofluo-
rescence. Representative photomicrographs
of livers of ATH diet-fed WT (C57BL/
10SnJ; A) and TLR4del(B) mice stained for
CD41-positive cells (original magnification,
20?; original scale bars?40 ?). Note the
relative paucity of CD41? red staining seen
in the inflammatory cells (C) of the WT mice
(original magnification, 40?; original scale
bar?20 ?).
1340 Journal of Leukocyte Biology
Volume 83, June 2008


text:
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text: Wedding Talk Matt Richenthal at October 27, 2013 8:58 am. Comments This is a romantic clip from "We All Fall Down," as Brooke and Julian talk about their wedding. Check it out now. Share Tweet Email Edit Delete Follow One Tree Hill Show: One Tree Hill Episode: One Tree Hill Season 8 Episode 4: "We All Fall Down" Related Videos: One Tree Hill Videos, One Tree Hill Season 8 Episode 4 Videos Uploaded by: Matt Richenthal Uploaded: October 27, 2013


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Always free shipping on orders of $25 or more!
A gel powder – what would that even look like? We'll tell you: It looks like rich, vibrant color that glides on in a single stroke, delivering a stunning multi-dimensional effect. What makes it a gel powder? Well, the formula starts as a gel and then is transformed by a special process into a stunning powder eyeshadow with pure color and power wear.
The unique process allows you to blend this incredibly innovative, weightless formula easily with your fingertips to light up your eyes. Even more to entice: It can be used wet or dry, resists creasing, and comes in satin matte, shimmer and iridescent metallic finishes. You just have to try this gel powder. It's that different.
Apply using ring finger or brush for luminous eyes in one stroke. Use lighter shades on brow bone as a highlighter, mid-tone shades on the lid and deeper shades for contouring in the crease. Use any shade WET as a liner to create intense color or added definition for limitless, multidimensional looks as unique as you!
Ingredients/Ingrédients/Ingredientes:Mica, Synthetic Fluorphlogopite, Silica, Aluminum Starch Octenylsuccinate, HDI/Trimethylol Hexyllactone Crosspolymer, Dimethicone, Isononyl Isononanoate, VP/Hexadecene Copolymer, Cetyl PEG/PPG-10/1 Dimethicone, Octyldodecanol, Magnesium Aluminum Silicate, Nylon-12, Caprylyl Glycol, Ethylhexylglycerin, Ammonium Acryloyldimethyltaurate/Beheneth-25 Methacrylate Crosspolymer, Polysorbate 20, Polyisoprene, Talc, Pentaerythrityl Tetra-di-t-Butyl Hydroxyhydrocinnamate.
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San Antonio, TX (PRWEB) August 05, 2013
A new teeth-whitening procedure effective on the teeth of the elderly and even teeth darkly stained by tetracycline has been introduced at Cosmetic Dentistry of San Antonio, cosmetic dentist Edward Camacho, D.D.S., has announced.
The KöR® Whitening Deep Bleaching procedure is now available at the practice, located at Huebner and Bitters in North Central San Antonio.
As teeth age, food stain debris becomes solidly packed between the rods of tooth enamel, blocking standard peroxide bleaching solutions from penetrating into the microstructure of the tooth, Dr. Camacho explains. This makes standard whitening results only partially effective and unpredictable.
"I have been disappointed with the results of other systems and it was much harder to predict the results," Dr. Camacho said. "KöR consistently gets great results and I am very happy to offer this unique system to my patients. Whiter teeth is the number one request from patients."
Further, researchers have found that conventional whitening trays are active for only a limited time, because the anti-oxidant enzyme peroxidase in saliva and other fluids quickly destroy the peroxide, he said.
The KöR Trays seal saliva and other fluids out of the whitening trays. In combination with the proprietary whitening gels, this procedure provides more than six hours of whitening, compared to the half-hour in standard procedures, Dr. Camacho said. This extended potency allows a thorough cleansing out of debris from between enamel rods, rejuvenating the enamel, more closely resembling that of a young person.
"I personally have tried all bleaching
|Source: PRWeb
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Book your room at Ranbanka Palace
Please enter your travel dates.
Get an overview of this hotel3-star hotel with full-service spa and free breakfast
Situated in Jodhpur, this hotel is 1.1 mi (1.8 km) from Sardar Market and within 3 mi (5 km) of Mehrangarh Fort and Jaswant Thada. Umaid Bhawan Palace is 3 mi (4.8 km) away.
Hotel Features
Along with a full-service spa, this hotel has a restaurant and WiFi in public areas. Free breakfast and free self parking are also provided. Additionally, dry cleaning, laundry facilities, and express check-in are onsite.
Room Amenities
Rooms feature minibars, room service, and TVs. Guests will also find hair dryers and safes.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
Ranbanka Palace features a full-service spa. The hotel offers a restaurant. Guests can enjoy a complimentary breakfast each morning. High-speed wired and wireless Internet access is available for a surcharge.
This Jodhpur hotel also offers tour/ticket assistance, multilingual staff, and a garden. Complimentary self parking is available on site.
Ranbanka Palace has designated areas for smoking.
- Free newspapers in lobby
- Designated smoking areas
- Dry cleaning/laundry service
- Elevator/lift
- Express check-in
- Express check-out
- Free breakfast
- Free self parking
- Full-service spa
- Garden
- Laundry facilities
- Luggage storage
- Multilingual staff
- Restaurant
- Safe-deposit box at front desk
- Tours/ticket assistance
- Train station pickup (surcharge)
- Wedding services
- Wired (high-speed) Internet access - surcharge
- WiFi (surcharge)
Room Amenities
- Air conditioning
- Daily housekeeping
- Hair dryer
- In-room safe
- Minibar
- Room service
- Television
Where to Eat
A complimentary breakfast is served each morning between 7:30 AM and 10:30 AM.
Ranbanka Palace has a restaurant on site.
Room service is available.
Spa Services
Guests can indulge in a pampering treatment at the hotel's full-service spa.
Hotel Policies
Check-in time starts at 2 PM
Special check-in instructions:
Check-out time is Noon
Payment types
Children and extra beds
- Children are welcome.
Pets allowed
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- Christmas Eve (24 December) Gala Dinner per adult: INR 5000
- Christmas Eve (24 December) Gala Dinner per child: INR 2500 (from 6 to 12 years old)
- New Year's Eve (31 December) Gala Dinner per adult: INR 5000
- New Year's Eve (31 December) Gala Dinner per child: INR 2500 (from 6 to 12 years old)
- A 5.15 percent city/local tax will be charged


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|ISSN 1088-6850(online) ISSN 0002-9947(print)
On the contact between complex manifolds and real hypersurfaces in
Abstract | References | Similar Articles | Additional Information
Abstract: Let be a real hypersurface of an open subset of and let . Let denote the maximal order of contact of a one-dimensional complex submanifold of a neighborhood of in with at . Let denote the for all tangential holomorphic vector fields with then where are positive integers such that and denotes the Levi form of evaluated on the vector field .
Theorem. If is pseudoconvex near then .
Retrieve articles in Transactions of the American Mathematical Society with MSC: 32F30, 53B35
Retrieve articles in all journals with MSC: 32F30, 53B35
Additional Information


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The URI Office of Environmental Health and Safety (EH&S) provides comprehensive training for personnel conducting Biological Research. Annual training is required for all personnel working at Biosafety Level 2 or higher on approved IBC protocols. This training covers biosafety guidelines followed at URI, the U.S. Occupational Safety & Health (OSHA)'s Bloodborne Pathogen Program, disinfection and waste disposal procedures, and the use of biological safety cabinets.
For staff or students new to URI, the first training must be completed during an in-person session offered by URI EH&S. Annual refresher training can be completed on the CITI program.
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I have a website that I've developed and tested using Firefox 9 exclusively. I'm pretty happy with the layout/styles when viewed in this version of Firefox. I'm now facing the unenviable task of making it display equally well (or as close as possible) in IE7+ (I'm not supporting IE6). Naturally, I'd also like it to display well in Chrome & Safari, but I think they implement the standards reasonably well, so I'm not so worried about them.
I'm using JQuery for JavaScript, which hopefully means I don't have too many differences in JavaScript behaviour, so my chief concern is the CSS. I imagine many others have been down this path, so I'm hoping there's a stylesheet available which when (conditionally) included will fix most common CSS problems seen when viewing a website in IE that has only been tested with Firefox. Does such a thing exist?


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Was/is Andoria ever a major space power?
View Single Post
January 15 2014, 12:09 AM
Location: Montgomery County, State of Maryland
Re: Was/is Andoria ever a major space power?
Bry_Sinclair wrote:
T'Girl wrote:
It's kind of a idiotic concept, the Andorian people have been around for multiple thousands of years with four genders, and suddenly
one of the prime reasons they're now going extinct?
There could have been some kind of radiation burst that affected them, or a mutated STI, or any kind of medical incident that has affected their biology which has only gotten worse over time.
A Ceremony of Losses
established that the Andorian reproductive crisis was ultimately caused by a mutation the species underwent thousands of years ago that gradually began causing more and more miscarriages and stillbirths.
And, no, there's nothing implausible about the idea of reproductive difficulties gradually causing a species to go extinct. Just check out the difficulties scientists have been having getting the panda species to reproduce.
Democratic socialism is the hope of human freedom.
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Exciting Changes Heading To Age Of Conan
Funcom today released the September edition of the Game Director's Letter highlighting some big changes for players of Age of Conan: Unchained. The letter highlights the massive undertaking during the server merge earlier this month, with over 6 million characters being transferred.
Joel Bylos also goes into great detail regarding the highly anticipated TradeSkill feature that now includes the ability to create 11520, including the opportunity to create your own weapon and give it any appearance you please from the long list of models available in Age of Conan.
Finally Joel also goes into detail regarding the upcoming Halloween event and the much awaited introduction of 3 new Unchained Dungeons, all of which are currently available to play on the TestLive server. For more information, or to read the letter in full, click here.
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Middle East recruiters expect new jobs in next 6 months
Naukrigulf survey reveals job creation and hiring much better in 2015 compared with 2014October 13, 2015 10:17
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MIDEAST STOCKS-Markets to rise on stronger global environment
Abu Dhabi's ADWEA in talks with banks for $350 mln project bond
Emirates hints at no Dubai Airshow orders, pushes decision on A350/787 to next year
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Qatar signed a deal with Royal Dutch Shell on Sunday to develop a $6.4 billion petrochemicals complex in the Ras Laffan industrial city in the Gulf Arab state.
December 5, 2011 10:13 by Reuters
Qatar's energy minister, Mohammed al-Sada, and Shell chief executive Peter Voser signed the agreement in Doha.
"We estimate the cost to be $6.4 billion but at this stage one should be cautious," Sada said.
Asked whether the agreement replaced a similar one signed with US group Exxon Mobil, Sada said: "No, this is not a replacement. This is a continuation of our strategy. There will be other petrochemical plants in the pipeline."
While industry sources said last year they believed Exxon had pulled out of the agreement, chief executive Rex Tillerson denied that and told reporters the company was waiting for Qatar to make its decision.
The plant agreed with Shell will have the capacity to produce 1.5 million tonnes of mono-ethylene glycol per year and 300,000 tonnes of linear alpha olefin, mostly for export to Asian markets, an official statement said.
State-run Qatar Petroleum will have an 80 percent equity interest in the project and Shell will hold the remaining 20 percent.
Qatar is the world's largest exporter of liquefied natural gas (LNG), gas chilled for export by ship. (Reporting by Regan E. Doherty; Writing by Firouz Sedarat; Editing by Dale Hudson)
US ready to help Syria after Assad Falls -Biden
December 5 2011
Syrian Authorities arrest woman blogger
December 5 2011


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Hello! Magazine
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THIS WEEK! Exclusive photo diary: Victoria Beckham speaks from the heart about her Ethiopia mercy mission. Plus Strictly's Natalie Lowe exclusively reveals her engagement joy. Plus Kate's sparkling night out. And exclusive: Singer Camilla Kerslake on why she and England rugby captain beau Chris Robshaw will never be defeated
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text: Kim Kardashian: Baby #2 on the Way? by Hilton Hater at August 8, 2014 7:38 am. Comments She's come out and said it! Kim Kardashian says she is trying for baby number-two. Share Tweet Email Edit Delete Follow Kim Kardashian? Facebook Disqus Star: Kim Kardashian Related Videos: Kim Kardashian Videos Related Post: Kim Kardashian Konfesses: I'm Trying to Get Pregnant! Uploaded by: Hilton Hater Uploaded: August 08, 2014 Duration: 73 seconds


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Show Schedule • Concerts • Gift Certificates
Junie B Jones
Awaken your child's desire to read with sassy little diva Junie B. Jones and her adventures in first grade. This is a delightful adaptation of Barbara Park's best-selling books brought to life in a genuinely comical musical. OCTOBER 3, 17, 24, 26, 31, NOVEMBER 7, 2015 Price: Breakfast – $16.00; Lunch – $21.00 Breakfast 9:00 . . Continue Reading
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Agatha Christie's And Then There Were None
The first hit play by Agatha Christie, the queen of murder fiction! This suspenseful mystery will keep you on the edge of your seat as one by one they disappear – until there were none.
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HOW GREAT THOU ART – The Gospel Music of Elvis Presley
Starring Robert Shaw & the Lonely Street Band From the earliest days of his boyhood until his untimely death, gospel music always held a special place in the heart of Elvis Presley. Before he became the most famous entertainer the world has ever known, as a young man Elvis wanted nothing more than to sing . . Continue Reading
The Return: A Beatles Tribute!
These four lads have skillfully recreated the Beatles experience and truly capture the energy and chemistry of the Fab Four. Doors & Buffet Open: 6pm Show Begins: 7:15pm Ticket Price: $38 "Front" $31 "Back" THIS SHOW AVAILABLE FOR PURCHASE BY PHONE ONLY. PLEASE CALL THE BOX OFFICE AT 812-288-8281. THANK YOU!
Irving Berlin's White Christmas
Based on the popular movie, this timeless classic is full of dancing, laughter, and some of the best songs ever written. A holiday musical comedy that is sure to be one of the highlights of your Christmas season!
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Jack Frost Saves Christmas
Follow Jack on his adventures in Christmas town as he discovers his special icy talents and manages to save Christmas! Santa will be at every performance. Price: Breakfast – $16.00; Lunch – $21.00 Breakfast 9:00 A.M. Show 10:00 A.M. Lunch 12:00 P.M. Show 1:15 P.M. NOVEMBER 14, 21, 27, 28, DECEMBER 5, 12, 19, 21, . . Continue Reading
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Honky Tonk Holidays
Decked out in their best holiday attire, Jason Petty and Carolyn Martin, backed by a five-piece band straight out of Nashville, perform the songs and tell the stories of your favorite country Christmas Classics such as: "Christmas Time's A-Comin", "Pretty Paper", "An Old Christmas Card", "Blue Christmas", "Little Toy Trains", "Christmas in Dixie", and many . . Continue Reading
EDDIE MILES presents "A Salute to Elvis & Country Legends"
Audiences will delight at Eddie's "Salute" which encompasses the Elvis Presley era and his transition to rock-n-roll from his country and gospel influence. Eddie will also perform selections from his other musical heroes such as Johnny Cash, Conway Twitty, and Marty Robbins. January 2, 3, 4, & 5, 2016 Doors & Buffet Open 6pm Show . . Continue Reading
THE MONARCHS present "A Tribute to Rock, Rhythm & Doo Wop"
Kentuckiana's favorite "oldies" rock n' roll band has put together a special show that will take you back to the days of sock hops and teen clubs. Relive your memories of the 60′s with the legendary Monarchs! January 8 & 9, 2016 Doors & Buffet Open 6pm Show Begins 7:15pm Ticket Price: $38 "Front" $31 . . Continue Reading
Southern Fried Funeral
Funerals bring out the best, the worst, and the funniest in people, and the Frye family is no exception. A big-hearted comedy about family, southern style! Written by Osborne and Eppler.
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Into The Woods
This Broadway musical hits the stage with an epic and modern fairytale about wishes, family, and the choices we make. An enchanting and wickedly witty Tony Award-winning musical featuring a score written by Stephen Sondheim.
Charlotte's Web
Based on E.B. White's classic book, this touching musical tells the story of Fern, her beloved pig Wilbur, and his best friend Charlotte. Come see why Wilbur truly is "SOME PIG!" Price: Breakfast – $16.00; Lunch – $21.00 Breakfast 9:00 A.M. Show 10:00 A.M. Lunch 12:00 P.M. Show 1:15 P.M. FEBRUARY 27, MARCH 5, 14, 19, 26, APRIL . . Continue Reading
FIVE VOICES – NO INSTRUMENTS! MO5AIC is a 5-man vocal supernova that will leave you scratching your head wondering where "the band" is. Having worked with the likes of Jay Leno, Prince, and Tony Bennett, MO5AIC's interactive and finely tuned performance is great for all ages and demographics. Date: March 21, 2016 Doors & Buffet . . Continue Reading
Legally Blonde The Musical
A fabulously fun musical based on the hit movie! Follow the transformation of Elle Woods as she tackles stereotypes, snobbery, and scandal, in pursuit of her dreams.
Chris Collins & Boulder Canyon presents A TRIBUTE TO JOHN DENVER
Hailed as one of the nation's most exciting tribute bands, the award-winning Chris Collins & Boulder Canyon present a tribute to the music and legacy of John Denver in a special concert event! With similarities between Collins' and John's voices, he brings to the stage the unmistakable enthusiasm that was a hallmark of a John . . Continue Reading
Pinkalicious the Musical
What happens when a little girl eats too many pink cupcakes? She turns pink, of course! See how Pinkalicious and her brother handle it in this hilarious musical based on the New York Times Best Seller. Price: Breakfast – $16.00; Lunch – $21.00 Breakfast 9:00 A.M. Show 10:00 A.M. Lunch 12:00 P.M. Show 1:15 P.M. . . Continue Reading
Melissa Combs presents A COUNTRY JUBILEE
Join Melissa Combs and friends for an evening of country music. Hear selections of classics, cowboy, gospel and swing. Plus, some laughs along the way. Sure to be a fun evening! Monday – May 23, 2016 Doors & Buffet Open 6pm Show Begins 7:15pm Ticket Price: $38 "Front" $31 "Back" THIS SHOW AVAILABLE FOR PURCHASE . . Continue Reading
The World Famous Glenn Miller Orchestra
The legend lives on as this 19 member band continues to play the original Miller arrangements. Enjoy a special dessert matinee or evening concert with dinner. Dessert Matinee Doors & Buffet Open 3:00 p.m. Dessert Matinee Concert Begins 4:00 p.m. Dessert Matinee Tickets: $25 each Evening Doors & Buffet Open 6:00 p.m. Evening Concert Begins 7:30 p.m. Evening Concert Tickets: $39 . . Continue Reading
The Van-Dells will take you on a nostalgic journey back to the 50's & 60's and offer a unique blend of comedy, choreography, and tight harmonies. When the Van-Dells hit the stage, it is non-stop action from start to finish! Monday – August 22, 2016 Doors & Buffet Open 6pm Show Begins 7:15pm Ticket Price: . . Continue Reading
"Classic Nashville Roadshow" takes you on a journey down memory lane with your favorite classic hits honoring the world's most beloved country greats! Starring Jason Petty and Katie Deal, "Classic Nashville Roadshow" features renowned duets such as Golden Ring; Louisiana Woman, Mississippi Man and Jackson; poignant tunes like Crazy and He Stopped Loving Her Today; . . Continue Reading


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unsteady states
Posted Apr 26 2009 10:45pm
As the weather finally warms up in the Pacific Northwest, I am finally past the worst of my allergy onslaught and am able to ride my bike more. And as my distances and the number of bike trips increase, I find myself wavering back and forth between two very distinct cycling states.
State One: I wear padded bike shorts, a jersey with rear pockets and stiff-soled bike shoes, and take the drop-bar road bike out for a longer ride. This ride is often done alone, though occasionally with faster friends who need a "rest day" ride and are therefore more willing and able to match my pace. We average speeds of 13 to 14 mph, which is on the speedy side of things for me. The reason I know how fast we're going is because my drop-bar bike has a computer on it, something I added when I started riding populaires a couple of years ago and needed a computer that was accurate enough to sync up with the cue sheets. On days when I feel limber and fluid, my pedaling is effortless and smooth and I enjoy the feeling of fleetness, even as I struggle to keep up with my faster friends. On the days when I'm wrestling with my perennially bad knees and my breath is wheezy from allergies, or I'm beset by too many bathroom stops, my pedaling slows and I feel frustrated. In this state, trying to be an athlete reminds me precisely that I am NOT one.
State Two: I wear street clothes, occasionally with special, padded cycling underwear but more often not, and I am more inclined to ride my city bike with its upright bars, racks and heavy U-lock. Although there is an old-school mechanical cyclometer on my front wheel, I usually have no idea how fast I'm going, and most of the time don't care. My flat-soled sneakers push BMX platform pedals with toe-cages bolted on, the toe-cage a holdover from my teenage years that I cannot let go of even in street clothes. I still wear a helmet -- I like my brain too much to take chances without it -- but the rest of my ensemble seems to give me permission to putter along, spinning in low gears and not worrying in the least when I am passed by every other rider on the road. I make many stops, at yard sales and gardens and friends' homes, and don't pay much attention to the clock. In this state I don't mind looking -- or riding -- like a non-athlete, a "
", a regular person with no pretensions to athletic greatness, and no concern about it either.
The odd thing is that I am unwilling to give up on either kind of riding. And so I waver back and forth, seeking out a new "athletic" cycling goal each year and trying it on for size. For the last two years it's been long-distance riding, hanging out with the Rando crowd. The rides, mostly on the west side of town, have been beautiful, and I've enjoyed -- or suffered -- my way through each. With distance rather than time being the primary goal, I've surprised myself and achieved things I didn't think possible. Now I know I'm capable of metric centuries (100k/62.5 miles) and have completed several of them. Will I try going for a 200k brevet? It's not clear, and lately it doesn't seem to matter so much. The fact that I've ridden SIX metric centuries in the last two years is amazing enough for someone like me, and knowing that I can go out and do it again feels good.
I have observed many lovely days in which I ride my bike just to ride it, and wonder if ultimately I can give up the desire for athletic "greatness" and just settle into a steady diet of State Two and stop worrying about being a jock already.
But in truth, I can't just let things go. This year, in what feels like another grasp at athletic greatness, I've decided to try my hand at cyclocross, that crazy sport where people buy fancy, knobby-tired bikes and run through the mud while carrying them over their shoulders. Why on earth would I even attempt this?
The awful truth: I grew up in an extended family of
, super-ordinary people who did not engage in anything athletic and who in fact were so sedentary that most of them grew fat and slow and horribly unhealthy. I came from people who were known for mental calisthenics, not physical feats of strength and agility. While I was certainly smart, I was also the odd child with the short attention span, the one who could not sit still long enough to read a chapter in a novel -- or even sit all the way through a hourlong TV show -- before I got itchy feet, "
", and had to get outside and just move around, climbing trees, wading through creeks and riding my bike all over town. Short and skinny and plagued by the recurring fatigue of a disorder -- Crohn's -- that would not be diagnosed until I was in my thirties, I sometimes nearly killed myself trying to do crazy stuff that perhaps I shouldn't have done, and never stopped dreaming of being a real athlete. I marched in drum and bugle corps, wilting in the heat and staggering under the weight of an enormous drum my wiry frame had no business carrying. I went out for track, ran the middle distances and sometimes collapsed from fatigue while trying to keep up with bigger, stronger kids. A running injury diverted my path towards bicycling, with twenty-mile rides in the country and
Breaking Away
and still more dreaming. And that dreaming is what has kept me coming back for more; more of State One and the lycra and the helmet that makes me look like an angry insect, more of the 60-mile rides and now this venture into the insanity that is cyclocross.
I come from a people with virtually NO history of athletic prowess; Hank Greenberg and a few other stars aside, Ashkenazic Jews are generally known for their brains rather than their brawn. My father was a child prodigy who studied piano at a conservatory; my mother was a writer, singer and would-be fashion designer. P.E. class was something to be suffered through, the only class in which a "C" would be a perfectly acceptable grade, and nothing more. When my physicality expressed itself my parents looked on in confusion, not really knowing what to do with their active younger child except to let her be. They never came to my drum corps or marching band contests, or to my track meets, but they did let me go for those long rides in the country and gave me money to bring back treats from the farmers' stalls. I grew still more wiry and tan and became a weird object of both admiration and envy for my parents, both of whom smoked, ate badly and were sedentary; and neither of whom lived to see 70.
When I look back on my beginnings, it is sometimes amazing that I have made the choices I've made -- to ride my bike as much as possible, to try my hand at unlikely things that make no sense and to see what I can do with this body before it gets too old to find out.
So this fall, look for me out on the cyclocross course. I'll likely be in last place, schlepping through the mud and dragging a cheap mountain bike behind me, stutter-stepping and hoping that, if I don't finish before getting lapped, I'll at least get filthy and have a grand time breathing hard and being among people who understand my need to get out and move.
(graphic designed by J. Edgar; t-shirts available at
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text: As we drove up to The Sea Whale I had very low expectations. We arrived at the motel just in time to watch the lovely sunset on the upper deck. I strongly suggest getting a room on the second level. The rooms on the first level looked like they would be very dark. Our room was clean. I did not... More


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we visit the cider press every year, staff are always very friendly, we sat in the window seats with the sun beaming down it was great, we sat there for hours having a game of...
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We called in there for a pint, not a good pint of cider at all, the bar and the toilets( ladies) didn't smell very clean, quite an odour in fact. Won't rush back, not the most friendliest of places
Having arrived on a Saturday evening about 7, loud music and 'merry fellas' dancing across the room greeted us
, went to get cask marque code and stared at making me feel uncomfortable, we didn't stay as hubby did not feel comfortable either, noise isn't a problem just intimidation, feels like a locals pub
This pub has been a good traditional fun pub over the years we have been coming to, since 1992. From owner to owner it's had a fabulous atmosphere.
In the last few years it's looking more and more tied though.
Lacking love and care, especially the toilets and furniture . When we went the last 4 years the toilets really...
we visit the cider press every year, staff are always very friendly, we sat in the window seats with the sun beaming down it was great, we sat there for hours having a game of cards, watching the world go by, we had been shopping,and didn't want to take our bags to the other pubs etc so staff was kind...
Went here for a drink one afternoon when nearby. Was a nice place for a drink. Recommend the rattlers on draft.
What a great pub.
We poped in for 1 on Sunday night and stayed for a lot more than 1:)
Live music the place was packed great atmosphere friendly staff.
Can't believe we've never been in here b4.
Will defo be telling everyone what a great little find this was:)
A couple of people sat outside, enjoying people watching and music playing – not to loud but you can listen to it. Walk inside this lovely quaint public house, where you're being greeted by a smiling bartender, who is happy to not only help, but advise on their vast selection of you guessed it cider very knowledgeable! They also do...
We always visit this pub on our annual trip to Torquay, this time there was a group of around 25 of us, we are always made to feel very welcome staff are always smiling and very polite, we had a great weekend the live music was excellent, we shall be returning next year
This is a lovely old-wordly style pub just off the main high street. The staff are very friendly and the pub is always warm and feels very welcoming. They have nice wooden tables and chairs to add to the ambience. The only reason i am not giving this establishment a 5 star rating is because i feel the prices of...
the cider press is a real pub just off the high st,nice wooden tables ,chairs and floors,a good selection of beers and spirits also wines,they do food but i did not eat,the place has a buzz about it clean and friendly staff,
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