text:
Refreshed, for another swing at the Cubs
Whichever way Hendry tried to spin it in the press conference, this trade is a rental. Matt Lawton is entering the final months of a four year deal that's paying him $7.5m this year. And when this season is done, Lawton, whose contract includes no options, will file for free agency. With Lawton turning 34 in November, and doubtless looking for a multi-year deal all the same, this being a rental or not, it's probably best left that way. But it is a rental. And that says much for the Cubs' attitude towards this season, it says that they still clearly think they can make the playoffs. But this team right now stands at just 53-52 and is very much flawed, as this lousy homestand against lousy Western opposition [Giants, Diamondbacks] has demonstrated. Nomar, Wood and Williamson, who Hendry is viewing as mid-season pickups, will not change that, promoting Van Buren won't either, and neither will Matt Lawton, whose simply not an impact ballplayer.The Cubs only gave up Jody Gerut, who managed just 18 plate appearances with the Pirates before succumbing to the problems with his knee that have ruined his season, but right now the Cubs' delusions of competitiveness mean that in the long-term they've exchanged him for Justin Berg, and that's just an unjustifable trade. In the short-term, Lawton gave Dusty Baker a convenient excuse to screw over Matt Murton, and the Cubs threw in a million dollars or so too. And for what? Parity with the Cincinnati Reds in 2005? I need a holiday.
Sorry with the multiple
Sorry with the multiple posts in a short period of time, ony my lunch break at college...
I loved this, the Top 10 reasons Scott McClain didnt get an AB yesterday...
# 9 is the best, as a reader
# 9 is the best, as a reader posted.
On Lawton, I always thought we'd end up with more for DuBois, and now am regretting we didn't just allow Gerut to get healthy and be a Cub next year.
For now Cubs, play the kids. Show Murton, Cedeno and any other young player for the rest of the season. Throw in Fontenot for some AB's, too. Start waiving Koronka, and some other AAA fodder and protect some of the real prospects instead.
Dusty's Top-10+ Reasons Why
Dusty's Top-10+ Reasons Why Scott McClain didn't get an AB Today
11) I thought his uniform number had been retired before the game started
12) Andre Dawson was in uniform and we were working on getting him a pinch hit appearance before McClain
Andy MacPhail was the
Andy MacPhail was the subject of two lengthy interviews over the past several days--one on WGN radio's "Sports Central" evening show; the other, the Score's "Hit and Run" Sunday morning show.
If I read Andy right, and he said essentially the same thing during both interviews, Baker and Hendry will be working through at least the 2006 season. There was not even a hint of a change of personnel at either position. Also, and just as important, MacPhail said nothing about a contract extension for either one beyond '06--at least not right now.
So, like 'em or not, it's looking more and more as though we'll have to live with Baker and Hendry for, in all probability, one more season.
In between all his management-speak, MacPhail did say he was extremely optimistic about '06, primarily because of the additional salaries the club will be able to take on next season.
Lawton traded? Who cares?
Lawton traded? Who cares? Didn't like DuBois. Didn't like Gerut. I like Lawton if we were able to compete. Since that's not happening, it was a great move. We cleared a lot of dead weight and now that the season is over we can start experimenting. Having said that, how many starts will Holly get in the next 30 or so games?
anyone hear anything about
anyone hear anything about Walker getting traded to cleveland?
Re: #6 -- just flipped on
Re: #6 -- just flipped on WSCR, I'll tell you in 5 minutes...
Nomar...is this a rumor or
Nomar...is this a rumor or do you think this just happened for real?
no hint of it on WSCR. so
no hint of it on WSCR. so it's just a rumor.
from what i can tell, just a
from what i can tell, just a rumor...
The Walker talk is just
The Walker talk is just rumor for now, nothign official.
For those who subscribe, check out Joe Sheehan's column on why the cubs have sucked this year. He identifies three problems:
1) Dusty's inability to recognize that neifi and corey were not 1-2 hitters
2) Putting rusch in the pen and dempster in the starting rotation, and then keeping rusch in the bullpen
3) Consistently playing the wrong LF'er
For all of their shortcomings, it's pretty much obvious to everyone that Murton should have been getting the bulk of the at bats since he's been called up. But instead we sank with Hollandsworth and other crappy players, and now not only have we wasted this year, but we did not get a chance to see what Murton can do on a full time basis -- even though his performanc evidences a solid contributor. Oh well, dusty is as dusty does...
Walker rumor was reported in
It hardly matters what we
It hardly matters what we get out of this trade sequence. It's all small potatoes. We overanalyze this not only because we overanalyze everything, but also because it takes our minds off losing. :P
13) I slept through Spring
13) I slept through Spring Training 2004
So...who does Cleveland have
So...who does Cleveland have that we would possibly get in return for Walker if that deal did come into fruition? I am guessing it would be mainly prospects(and don't tell me dubois). Anyone know if they have any young studs?
So...who does Cleveland have
So...who does Cleveland have that we would possibly get in return for Walker
"Bear" Bay? :)
"For all of their
"For all of their shortcomings, it's pretty much obvious to everyone that Murton should have been getting the bulk of the at bats since he's been called up."
obvious to who? if youre trying to win you surely dont stick him out there every day for the hell of it when you have MATT LAWTON on your club.
did he mention that TODD HOLLANDSWORTH had just come off a .360 clip when he was being played along with murton/lawton?
sounds like a buncha crap to me.
and what's so special about the dumpster that he deserved the closer role besides 1 offhand comment by hendry and a few by sportswriters?
and what's so special about what dumpster has done aside from a month and a 1/2 of effitive ball surrounded by pure crap?
and did joe shee. convienently forget the cubs own one of the worst bullpens in baseball (sans arizona who is just beyond awful) and has carried 1/3rd-1/2 of their lineup with .300 ob% hitters at any given point in the year?
that seems like a major oversight to ignore that.
Crunch, for all your
Crunch, for all your complaining about the Bullpen, etc, do you really think that fighting to stay out of 5th place is the best this team can do?
While the Cubs have holes, and have had them all year long, they really aren't much worse than the holes that every team in the NL East has.
Sheehan didn't talk about
Sheehan didn't talk about the bullpen (except to point out that Rusch didn't belong there) but he did offer some helpful hints on where these .300 OBPs should bat. Hint: not leadoff.
"The Cubs needed a better bullpen" isn't incompatible with "The Cubs certainly didn't use the rest of what they had very well," which was Sheehan's focus.
what does i think have to do
what does i think have to do with what this team has to work with?
and what does what you said have to do with the points i raised above?
an issue was raised about dempster and murton and i addressed them via what ive seen and backed it up with what's been done by the players leading up to the event.
looking at murton vs. righties...looking at what holly did leading up to murton joining...looking at the fact matt lawton is on the club...i drew those points.
looking at what this pen is...looking at the fact this pen has been crap ALL year...looking at the fact the dumpster puts 1.50 guys on base an inning...i drew those points.
now, what's your points besides saying "but..but..they did this!" ?
okay ron...thank you. that
okay ron...thank you.
that was very unsheehan-like to absolutely ignore things and come to those 3 points.
tem...if you have an agenda, its not nice to hijack someone else's name to attach your views to it.
this whole rant could have been avoided...
yes, batting corey/neifi 1/2 was retarded...yes, rusch has been worse as a starter compared to a reliever (but its not like rusch the starter has been all that great)...yes, dusty's made some weird moves like most managers in the game do.
anyone see florida's lineups the past few games or their substitutions?
castillo burried with conine batting 2nd? seriously, this stuff happens a lot..its when it happens for a majority of games that stuff gets really weird.
people already giving cedeno a death sentence even though he's playing...people wanna give murton the LF job with lawton already around as if he was traded for to be a bench player and he has no history of success...
There is nothing that CLE
There is nothing that CLE has in the minors outside of that Aubrey kid I would want. I imagine it would be for Dubois since Hendry likes him and I think he regrets that set of trades. I have to give it to you John your analysis on the Lawton trade was on the mark. I still don't think Gerut is that great, but he will probably be better than whoever the 4th OF is next year.
whatever anyone's interest
whatever anyone's interest in walker is...they got 2 days and a few hours to get it done if its likely to get done.
You mean "THE" Matt Lawton??
You mean "THE" Matt Lawton?? Wow..you are right Crunch....
If Lawton played woth a shit, sure. But he stunk with the Cubs, period. Jollandswoth's big tear? 2HR and 16 RBIs..geez...better trade Burnitz and K-orey...Oh, that was an aberration..as May was .111, with .179/.139/.318...July was .197 with .260/.348/.608..yep, he's a keeper.
Murton may need work hitting righties, you are correct. But calling him out like he was Roosevlet Brown on such a small sample size is ludicrous. (4/20)
dustyb... yeah...the matt
yeah...the matt lawton...the guy with who the club traded for...the guy with a history of success. yeah, that one. ever heard of him?
and no matter what you wanna say about holly he was still coming off a .360 clip. no amount of whining will take that away. no amount of whining will also make murton's obvious holes vs. righties suddenly get better.
why is one person's crap better than someone elses?
did murton step up and dominate both sides of the plate? no... did he get a chance to see pitchers from both sides? yes...
did murton show up in baseball 3 months ago with no one knowing about him? you think he was in the minors for 1000-ish a/bs without anyone noticing how he handles righties, especially righties insides with breaking stuff? do you seriously think that? i think you do.
murton isnt a 19 year old that was drafted in june.
you think he was in the
you think he was in the minors for 1000-ish a/bs without anyone noticing how he handles righties, especially righties insides with breaking stuff?
Murton hit righties just as well as lefties in the minors.
Hey chifan3887, I am still
Hey chifan3887, I am still will to make that bet that the Cubs will win 65 games. What do you think?
its not what your #s
its not what your #s are...its how you get them and what youre getting them off of.
he's faced A, AA, and non-pro pitchers his entire life.
based on the A and AA ball ive seen, and that's a whole lot...you're not gonna see refined sliders and changeups down there.
if anything can be learned from dubois and rich hill (to keep it recent) there is a HUGE difference between major league pitchers/hitters vs. what you see in the minors.
Doh. Yup...I can type! I
Doh. Yup...I can type!
I am still willing to make that bet...
btw...cedeno's move through
btw...cedeno's move through this cubs system has NOTHING even remotely to do with his #s...if it did him and l.montanez would probally be teammates throughout the both of their careers.
there's more to the minors than numbers by far...
Crunch: What exactly are
What exactly are you trying to say about Holly? That he deserved more playing time?
He had an admitedly hot June, hitting .360/.372/.547 in 75 at bats. Even after Murton's call up, though, he had almost the same number of at bats in July (65) and put up horrible numbers: .197/.260/.348.
I neither have a secret
I neither have a secret agenda nor did I attach someone elses name to my viewpoint. I menionted that Sheehan made 3 points, paraphrased said points, and then in a new paragraph typed my own. Do I need to put "In my opinion" in front of every f'n thing in write? I have no agenda, and even if I did, do you really think that Hendry/McPhail read these message boards and say "shit TEM, some random poster on an internet message board is shitting on dusty baker, we better get rid of dusty to appease TEM." Get over yourself; you're not that important to the cubs, and neither am I. Christ you sound like Dusty Baker blaming the Internet for his problems instead of looking in the mirror.
The point of my comment was to show that to the statistically oriented community, Dusty has wholly misused the players on the team, even when provided better options. Hollandsworth has had one decent stretch this year that lasted about 2 weeks in june, and over the course of his career has been no better than a 4th outfielder. Murton clearly showed that he should have been given a shot at the everyday position, BEFORE they acquired Lawton. Oh shit, looks like i forgot to write "in my opinion" in front of this again, maybe you'll attribute this to Hunter S. Thompson.
no im not saying holly
no im not saying holly deserved more playing time...
im saying ignoring that he was producing at the time is akin to ignoring the fact that murton isnt a complete hitter from both sides of the plate.
there's a lot more righties than lefties around and 3/4 of the year a guy's gonna see those righties.
its a complete package going against murton for why he wasnt there everyday...the main being the addition of matt lawton above anything holly can/would/did do.
big lowitzki, I never said
big lowitzki,
I never said the Cubs wouldn't win 65 games unless your COL, KC, TB, Pit you should be able to do that.
What you and Baker want is a guy like Cabrerra who is ready to go when he gets the callup. Guys will have holes in their game and a good manager lets a guy go out there and work through their problems. If you and Baker were running this team in the early 80's Sandberg would have never became Sandberg because you would have traded him after he went 0-32.
tem...you posted 3 points
tem...you posted 3 points and attached them to shee...
not all of us frequent the "baseball prospectus" nor do all of us pay for it...
you presented 3 points, 1 of which was outright debunked totally.
that is taking someone's name and attaching your view/interpretation/etc. to it.
"For those who subscribe, check out Joe Sheehan's column on why the cubs have sucked this year. He identifies three problems"
that says
1- sheehan identified those points
2- sheehan's points were 3 points
the paragraph afterwards i took as your opinion and covered that in another part of the post.
Actually, those are three
Actually, those are three points that Sheehan made, and none of them were debunked.
I posted this in another
I posted this in another thread but I thought I'd throw it out again just to give my 2 cents worth...
Although the trade for Lawton didn't work out in the long run, at the time it was the right thing to do if you still felt the Cubs had an outside chance to win this year. With over 2 months left in the season, I wasn't ready to throw in the towel, not with the guys we had coming back for the stretch. And you can be damn sure that Hendry, McPhail, Baker and others with reputations and pressure on the line surrounding a $100 Million payroll weren't ready to throw in the towel either.
With all that being said, it didn't work. So in retrospect, maybe we shouldn't have picked up Lawton and just let the young guys play. But you can't bitch constantly about the team not having a legit leadoff hitter with a high OBP (which historically Lawton has had) and then bitch when we pick one up at the trade deadline for a guy that should be nothing more than a backup OF'er for a legitimate contending team. There are many reasons why this team has underperformed, but the chain of events that ultimately produced us Justin Berg, in my opinion, is not high on that list
"If you and Baker were
"If you and Baker were running this team in the early 80's Sandberg would have never became Sandberg because you would have traded him after he went 0-32."
in fact if you'd bother to research what ive said about murton in the past you'd learn i compare the two a lot.
but that would require research insted of just saying "its dusty's blah blah"
its a lot easier to blame 1 guy for the shortcomings of 25-40 players.
"Actually, those are three
"Actually, those are three points that Sheehan made, and none of them were debunked."
well tem...
in that case...refer to the 1st responce where i called shee shortsighted and ignorant to what more pressing problems were.
and if im wrong, im sorry...i dont pay BP a dime to read their stuff, nor do i plan on it.
if shee did say that...refer to the 1st post responce and i appologize to you.
Sheehan's support for his
Sheehan's support for his contention that Rusch should've been in the starting rotation is his performace as a starter as well as the lack of performance by Dempster in the starting rotation (as well as Koronka and Hill).
You may not subscribe to BP, but you should really rely on something more than hearsay to try to refute something.
what exactly did shee
what exactly did shee say...im getting 2 stories...tem's and rob's....
someone's either misinterpreting or someone's lieing.
im not asking for a full
im not asking for a full report of a copycontrolled and for-pay article...but im curious to know what was said since im knee deep in this crap now.
Hey Chifan, Cabrera hit
Hey Chifan,
Cabrera hit something like .220 for his first month as a starter, and .250 for most of the year in 2003. And, he's a liability in the outfield. He would never have been given a chance to play on a Dusty-led team after showing those kinds of numbers. Rookies have to produce, you know.
My response to your points
My response to your points then:
Re: Dempster & Starting & Closing. I'm not saying he should have been made the closer right away, but that doesn't mean he should not have been in the bullpen. Look at Dempster's starting numbers over his career, he's been less than stellar pretty much his entire career (save one year) whereas Rusch has outperformed him as a starter both last year and this year.
Re: Murton & Hollandsworth. Hollandsworth has had one good stretch that lasted 2-3 weeks this year. Over the course of his career, he has shown to be nothing better than a 4th outfielder. Murton, however, got on base at a .415 clip, and this team sorely needed players who got on base. I've heard you repeat ad nauseum that he can't pull the ball, blah blah, as if hits to right field somehow don't count, and that getting on base via the walk does not count. Further, you say he can't hit righties, but what evidence do you have to support that? Murton has been a solid hitter of his career in the minor leagues, and good minor league performance EVIDENCES the potential to hit at the major league level
I'm not slamming the trade for Lawton or Lawton playing over Murton. I'm attacking Murton's lack of play before that acquisition - although Murton should have stayed up on the roster when Hairston returned from the DL to at least play against lefties (whom he mashed) while Patterson should have been sent back to AAA.
Finally, Sheehan never said if Baker had made the moves that he suggested, that the cubs would be in 1st place, just that those moves contributed to the poor performance of the team this year. You're reading into his comments far too much because you disagree with them.
rob=ron...for clarification...
to take a buncha points and come to those 3 conclusions...that just dont sound like shee to me.
to say those 3 reasons are why the club is sucking is so shortsighted to me compared to bigger issues seems like a joke.
rusch/dumpster was solved after a month and it didnt do a whole lotta good as it is.
constantly playing the wrong LF...for over 1/2 the season that was holly vs. dubois and that's 2 flavors of suck.
i just see a bunch of nothing in those points (aside from 1) and a lot more pressing issues that contributed to the cubs decline.
why are those 3 points given and not the others when summing up a death sentence for this team?
that is my question...
5 for 20 is too small a
5 for 20 is too small a sample to simply say "he can't hit righties."
about dumpster... his WORST
about dumpster...
his WORST area of performance is pitches 1-15
if anything this should say he's not closer material since that's historically where he gets lit up.
that's a hell of a gamble, period.
"The gap between Cubdom and
"The gap between Cubdom and contention is wide, but so much of it is contained in three Baker mistakes: using Neifi Perez and Corey Patterson atop the lineup, refusing to play his better left fielders, not keeping Rusch in the rotation"
morphesus... get this
get this tatoo'd on your eyelids so when you close them at night you can say 'oh yeah...'
murton didnt show up 2-3 months ago.
he's had almost 1000ab's in the minors that have given people plenty of looks at his stregnths and weaknesses.
guys just dont show up without a scouting report on them...maybe the other team dont know them, but that's why advanced scouts exist.
advanced scouting told other teams to pound him inside, and they have. this is a problem with his game. seeing as a guy is gonna see a righty 3/4 of his ab's in the majors unless he's a bench player, this is a major issue.
hell, murton rules...he just dont rule right now. he's damn close. hopefully he's not got a choi-like block on inside stuff. pat listach (ha) is working DAMN HARD trying to sort him out in the limited time he has him. this will be his 6th hitting coach he's seen (at least) in the past 3 years.
"The gap between Cubdom and
"The gap between Cubdom and contention is wide, but so much of it is contained in three Baker mistakes: using Neifi Perez and Corey Patterson atop the lineup, refusing to play his better left fielders, not keeping Rusch in the rotation"
thank you tem...and i appologize for my off the cuff comment earlier. if that is the grasp of it and it seems i was totally 100% wrong.
my beef is with shee's shortsighted article it seems.
Crunch, I don't think you're
Crunch, I don't think you're getting two stories. Sheehan detailed ways he feels Baker's misused the talent on the team. He didn't talk about the bullpen except to acknowledge that it was subpar, and the bits about Rusch/Dempster. You're saying "The Cubs had serious problems in the pen," which I'm sure he'd agree with, but Sheehan's article focused (in the BP style) on how they didn't use what they had in an intelligent fashion.
His summary:
It's dangerous to oversimplify this, but so much of the Cubs' deficit in the wild-card race can be traced directly to their shortfall in runs, and that deficit to poor lineup construction, that it's hard not to just level a finger at Baker.
Baker continues to show little understanding of how an offense works, of how to fit his players' skills to the proper roles. Worse, he shows little desire to learn these things, making the same mistakes repeatedly while taking little criticism for his decisions, and deflecting what criticism comes in a manner that has nothing to do with baseball. Whatever his leader-of-men qualities may be, it's increasingly clear that they're not enough to make up for his decisions.
He's not saying "this is why the Cubs are bad." He's saying "the Cubs' tactical management and use of their roster were bad enough to put them 7 down instead of 3 down."
I too think he overplays the LF hand, but the other two, Neifi/CP and Rusch, are varying degrees of slam dunks.
Take it easy, Crunch. Why
Take it easy, Crunch. Why are you so uptight? All I said was that we're looking at an awful small sample size in the major leagues. You are fond of saying that it's completely different in the majors than in the minors, right? So, how do we know that Murton has this huge hole until we get more than a few AB's to evaluate him on? He hit both righties and lefties in the minors. Did you see the advance scouting report on him or something? I know you have connections in the business... and I'm not being sarcastic there.
"refusing to play his better
"refusing to play his better left fielders"
dubois? hollandsworth? gerut? murton?
what better left fielders?
you got a lefty specialist and 3 flavors of crap as far as 05 goes.
i guess hairston could be out there if someone wants to make an arguement for him, but he spent a lotta time spelling todd walker at 2nd when he missed a month of action and spelling corey in CF when he was sucking hard.
"Take it easy, Crunch. Why
"Take it easy, Crunch. Why are you so uptight?"
im not uptight, i just covered the answer to that from my view in this very thread already.
and i like to inject jerk-like humor in my posts sometimes hehe...i got nothing against you.
"So, how do we know that Murton has this huge hole until we get more than a few AB's to evaluate him on?"
okay...now that i answered and re-answered...re-answered in the very post you're complaining about.
he HAS been evaluated, people KNOW what's up with him.
a lot of people get paid (really bad pay, btw) to know these things.
those binders in the dugout with other team's names on them...theyre full of this stuff.
"He's not saying "this is
"He's not saying "this is why the Cubs are bad." He's saying "the Cubs' tactical management and use of their roster were bad enough to put them 7 down instead of 3 down.""
thank you ron...this whole thing has escaped my grasp and yeah, i took the whole thing the wrong way.
the lack of clarification in my head between what i was getting caused me to hit on the wrong points of a greater issue.
if you feel slighted, i appologize to you, too.
and once again, i appologize to you tem.
...and yeah, Neifi/CP was a slam dunk point
no worries, it's an internet
no worries, it's an internet message board. can't really take these things seriously :)
well, anyone that runs his
well, anyone that runs his mouth as much as i do will make mistakes.
if anything, especially when i involve the community here, i must accept responsibility for saying stupid crap.
thank you for accepting my appology and i seriously do extend it to you and anyone else who had to sit through this.
in the future i need to do my own homework rather than interpreting the back/forth because we dont all use the same venacular to say the same things...interpreting can lead to error and i really made a big one in this post.
when push comes to shove and all opinions of this club aside...we're all cubs fans...i think =p
thank you for accepting my
thank you for accepting my appology and i seriously do extend it to you and anyone else who had to sit through this
We had deal with this season I think we can handle your posts.
big lowitzki, I never said
big lowitzki,
I never said the Cubs wouldn't win 65 games unless your COL, KC, TB, Pit you should be able to do that.
You are right...sorry. it was Cubby78.
Cubby78...do you read this? Or was that just a one week thing?
Sometimes I wonder if
Sometimes I wonder if anybody here is actually paying attention. Just to set the record straight, RYAN DEMPSTER has done a quality job as a Cub reliever.
As a relief pitcher this year he:
Has a won-loss record of 4-0
Has converted 19 Saves in 21 opportunities
Has an ERA of 2.59 in 40 games.
Has a WHIP of 1.34
Has a GO/AO ratio of 3.5
Has limited opponents to a .222 batting average.
Sheehan is a big Dusty fan
Sheehan is a big Dusty fan (NOT!), but actually, he was negative on the Cubs at the beginning of the season (and 2004 as well) because he did not like the way they had constructed their offense (to many guys with low OBP and not enough left handed hitting). With the departure of Alou and the disappearance of Sosa we knew the outfield was not going to be as productive as last season, and when Patterson became the worst offensive starter in the NL outside of Christian Guzman, the offensive liability of the Cubs outfield became fatal. And it was not particularly good defensive outfield either, especially when Macias and Lawton were out there.
Crunch, the bullpen problems were to some extent set up by Dusty's abandonment of the original plan (Dempster as the closer with Hawkins as the set up man) when Wood and Prior got hurt in spring training. And that set of dominos started tumbling because the Cubs let Clement go and did not sign a starting pitcher to replace him and provide depth, something they should have done given Wood's and Prior's history of health problems. So the supposed Cubs' strength, starting pitching, became a weakness, and less innings the starters pitched, the more the bullpen got exposed.
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Recent comments
La Stella (3B) batting fifth. Could be the La Stella game.
[Drafts plans for CTSteve idol.]
Pitcher batting 9th. Bryant in left, Schwarber in right.
i'm not ready for it to end.
i'm not ready for "well, they weren't supposed to make it this year..."
i'm not ready to talk about the off-season yet.
I can relate to that, E-Man. I don't think I'll enjoy watching a high-stakes away game like this on TV. I've watched a lot of games this season after they ended or at least after the Cubs got out to a decent lead. Since they usually win, I've gotten to see a lot of baseball.
But in this case I think we should probably all watch the game live, and not let the pressure exceed the pleasure.
Remember, as I told my wife when our kids went off to college -- this is a good thing! Enjoy it!
Fully agree -- can't prove it. But, the numbers are what they are, and a lot of his OF games have come in Aug & Sept, when he has been killing the ball otherwise. And, knowing how baseball players love routine, it seems logical that it could at least be a a factor.
Really, really, really hope I'm wrong on this.
Indeed! It has been absolute blast -- from getting swept by the Phillies to sweeping the Giants, I have always enjoyed being part of this group. Hope we have a lot more games to talk about this year.
Yes, cheers to me! I've noticed a fairly strong correlation between my tenure and Cubbie victories.
And thanks to Michael for our new diggs--and to y'all for sticking around!
CHEERS to CT Steve for keeping this alive in 2015. Who would have thought that the season would have been so successful to this point? Thank you CT STEVE!
I think I may be too nervous to watch the entire game. I will probably watch in bits and pieces - and constantly check MLB App. I will be like Blockhead: Nervous Nellie.
You can't prove the performance is because he played the OF. You just can't. Sorry.
BTW, Bryant hit .168 in July playing 3B exclusively.
oh man, early on it seems like he made at least 1 stupid baserunning blunder per game and almost every single one turned into a positive for the cubs. it was magical.
Not surprised about Lester but bit surprised about Hendricks. Guessing that if something happens to Arietta right away they go to Lester but if they've burned relievers and are in extra innings they go to Hendricks.
My kids' 8:30pm (ET) bed time will be waived tonight (They are Rizzo & Castro fans). We'll get geared up in our Cubs apparel and will watch this as a family. I am stoked. Go Cubs!
Bryant: Base running adventures.
Remember Bryant's home-away splits, too.
Wrigley: 21 HR, 59 RBI, .311/.408/.629
Road: 5 HR, 40 RBI, .243/.333/.360


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3:10 AM, May 11, 2013
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Rainfall expected to last until the early afternoon today could take away 6-year-old gelding Demonstrative's edge and leave the 72nd running of the three-mile Grade I stakes race at Percy Warner Park
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Mars Candy Coupons | As Low As $0.34 Starting 1/12
Be sure to grab these Mars Candy Coupons as they will make for some great deals at Walgreens starting 1/12! If you are a chocolate lover or just like to have candy on hand for the kids, be sure to take advantage of this great deal where you can get Mars candy bars as low as $0.34 each![pl-deal id='212741′]


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Hi Hervé,
Very nice view of the coast. Cotton-soft sand scattered waves.Very nice presentation and sharing.
I added you to my favorite list.
Best wishes, İsmail
thank you, I am happy you like this picture, Hervé
nice coast, good shot, LIKE, best wishes
Lloret, merci et bonne soirée, Hervé
Photo taken in Le Fort du Loch, 56520 Guidel, France


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Scary scenes from Fright Nights at Playland in Vancouver, BC., October 8, 2015. The adult themed halloween event which opens on Oct 9 features seven haunted houses with names such as Asylum and Keepers Doll Factory. The show involves as many as 99 actors covered in 3 gallons of stage blood, wearing airbrushed silicone masks that take 30 minutes to apply.
Alcohol is belived to be a factor in an accident Thursday night that saw a white Corvette plow through a bus stop in Surrey.
My wife was hoping for Brad Pitt to be on the bed when we opened the door to our room at the just-opened Palladian Hotel.
When I tell people that we counted 27 cowboy hats during 10 days in Texas, the usual response is, "Is that all?"
The idea of following one's dream pervades California culture.
Even if we don't particularly look like models, we feel like models.


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Australia was colonised as Terra Nullius, empty land when it wasn't. Palestine was colonised under the lie that it was empty when it wasn't. Difference is Australia has created one state with equal rights for all while Israel has pushed her indigenous people into concentration camps and bantustans and given second class citizenship to som because they are religiously inferior.
from the article: The Arab villages that were: a new Israeli guidebook


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John Rossman
Moss & Barnett, P.A.
John K. Rossman is a shareholder and Chair of the Creditors' Remedies Practice Group at Moss & Barnett, P.A. Mr. Rossman is a nationally acclaimed authority on the Fair Debt Collection Practices Act and the labyrinth of laws that impact the debt industry. He is a counselor and advisor to national and international companies and noted for his intelligent, creative and successful representation of collection agencies, debt buyers, creditors and fellow attorneys in cases across the country.
Network with John Rossmanon LinkedIn
Recent Posts
Radical State Law Changes Constrict Collections in New York, Illinois and Maine
In the latest episode of the Debt Collection Drill, attorneys John Rossman and Mike Poncin break down the law changes in New York, Illinois, and Maine, and provide practical guidance for compliance and avoiding pitfalls.
Another Reason For Collectors To Be Cautious When Calling Consumer Cell Phones
Given the data that must be weighed by a creditor or debt collector in determining where a consumer resides – the area code of the number called, the zip code of the residence of record, any statements by the consumer about his or her place of residence – it is certain that the Discover Consent Order will be the start of yet another flood of consumer lawsuits against the collection industry regarding the calling of consumer cell phones.
What Debt Collectors Really Need To Know About the FCC Order and the TCPA
The recent order issued by the FCC regarding the TCPA encompasses 138 pages (including hundreds of footnotes) and created an instant tidal wave of questions, comments and uncertainty about the use of telephone technology to contact consumers on mobile phones. After digesting the order, Moss & Barnett attorneys John Rossman and Mike Poncin share what you need to know.
Is It Illegal to Charge Credit Card Convenience Fees?
Charging convenience fees: should you or shouldn't you? The practice might be the next battleground for the debt collection industry. As with many regulations, there are more questions than answers — and the answers that are available aren't to everyone's liking. Join attorneys John Rossman and Mike Poncin, of Moss & Barnett, to get another point of view on fees and debt collection.
Debt Collection Drill: Common Sense Prevails in West Virginia?
The West Virginia Consumer Credit Protection Act, and how it was interpreted by the consumer attorneys in West Virginia, was the primary reason that State had previously been described as one of the most "treacherous" places in the country for debt collectors. Lawsuits were routinely commenced in West Virginia State Courts seeking hundreds of thousands […]
ARM Firms Should Assess Multidistrict Litigation for Simultaneous FDCPA and TCPA Cases
Collection agencies, debt buyers and credit granters are often under siege, forced to defend against identical claims on multiple jurisdictional fronts, regardless of whether the claims are on behalf of an individual or a putative class. One strategy for consolidating the defense of identical claims is to file a motion with the U.S. Judicial Panel on Multidistrict Litigation (MDL) to transfer claims to a single venue.
Court Expands FDCPA's "Least Sophisticated" Standard to Include Attorneys
A long-established exception to the FDCPA's "least sophisticated consumer" standard has been communications with consumers' attorneys. Because how could it be argued that an attorney is not "sophisticated?" But a recent Circuit Court ruling opened new ground on that front when it found that some communications with attorneys should be held to the standard.
Is There a "Silver Bullet" for Defeating FDCPA and TCPA Claims?
Collection agencies and debt buyers continue to be inundated with FDCPA and TCPA lawsuits, many of which drag on through months and even years of expensive discovery and motion practice. What if there existed a single argument that could be made in many consumer cases that would successfully remove the matter from Court and likely end the case in its entirety?
New York Debt Regulations Create Chaos
The New York State Department of Financial Services announced recently revised debt collection regulations, culminating more than a year of proposals and comments. While the new regulations provide clarity and consumer protection in some areas, they are fraught with ambiguities and overlap existing laws in several key aspects
Defeat Claims That Your Envelopes Violate the FDCPA
In the weeks since the Circuit Court decision in Douglass v. Convergent — the infamous envelope window disclosure case — filings of similar cases against debt collectors have been brisk. Two ARM defense attorneys discuss some specific legal theories upon which debt collectors may defend similar claims.


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Kamran Shirazi used to play at the Santa Monica Chess Club when I joined it in 1979. He was a slim, young elite player we were all terrified of. As for the Petroff, that "dull, drawish" opening can result in a blood bath right in the center of the board!
Why don't you just keep him delayed and wait for the prefect opportunity to do a late Scholars Check
That always works when I play at School
The Caro-kan defense is a good oppening in response to E4
The main line is
Forget about 1.e4 and 1.d4. Learn to attack from the corners. Join us in the vote chess games of these groups;
1.b3 Nimzo-Larsen Attack
1.Nf3 > c4 Reti
KIA inc.
We'll show you how.
Here is a good starting move aganist 5 to 10 out of ten players.
1.Move the kings pawn forward 2 squares.
2.Your oppenant should mirror your move to stop you advancing any further (Move their kings pawn forward two).
3. You then move you queen out as far diagnal right as it will go.
4. Your oppenant should move their pawn forward to attack your queen.
5. Slide your queen left and take there pawn. They are in check and have to etiher move their bishop or queen in front.
6.When they have done this take their kings rook.
If they stuff up again you will also be able to take their knight!!!
it kick arse!
other things you can do are
1. 4 move checkmate your oppnant
2.Move the pawns infront of your king and queen forward 2 squares to let out your bishops and queen. Notice that if you only move them forward 1 square they block off your bishops.
3.Scare them into forfiet!!!
4.Do what i do kick arse!
You can also put bishops and queens attacking pawns so that if they move the pawn their moving into check or
attack the pawn dignally across from their rook so that if they move it you can take their rook.
Get chess master it has a lesson section. Chess titans (vista) is good for a game.
Mr. Madison, what you have just said is one of the most insanely idiotic things I have ever heard. At no point in your rambling, incoherent response were you even close to anything that could be considered a rational thought. Everyone in this room is now dumber for having listened to it. I award you no points, and may God have mercy on your soul.
Two suggestions: (1) Always play opponents with higher ratings than yours. Have the computer analyze all your games. You'll learn that way. (2) Play the English Opening. No matter what your opponent does, you can't lose in four or five moves! It gives you time to make plans.
1.c4 e5
2.Nc3 Bc5
3.g3 Qf6
It's not THAT simple.
I like GK's openings. You can check out his games: http://chessgames.com/player/garry_kasparov.htmlLasker's games: http://www.chessgames.com/perl/chessplayer?pid=19149Or my grandfather's famous match:http://www.chessgames.com/perl/chessgame?gid=1243095
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Live updates
- ITV Report
Boy trips and puts hole through million-dollar painting
A 12-year-old boy holding a drink stumbled and tore a hole in a 17th-century oil on canvas by the Italian master Paolo Porpora in Taiwan.Read the full story ›
- ITV Report
Taiwan plane crash pilot 'turned off wrong engine'
The pilot of a TransAsia plane switched off its only working engine seconds before it crashed, a new report has revealed.Read the full story ›
- ITV Report
Taiwan water park explosion leaves hundreds injured
A fire at a water park in Taiwan has left more than 500 people injured.Read the full story ›
Ten TransAsia Airways pilots suspended after crash
Taiwan's aviation authority has ordered the suspension of 10 pilots from TransAsia Airways after one of it's planes crashed killing at least 42 people.
The Civil Aviation Administration said the pilots failed proficiency tests on handling emergency situations given after the crash and need to be retrained.
It said 19 other pilots who did not take the test would also be suspended.
The plane crashed minutes after take-off from Taipei's airport. Fifteen of the 58 people aboard were rescued and one person is still missing.
TransAsia Airways offers crash victim's families £308,000
Taiwan's TransAsia Airways has offered to pay compensation to relatives of those who died in a plane crash in Taipei last week.
Each of the families will receive £308,000 if they want to accept it after 40 people died on February 4.
Dramatic footage of the plane coming down shortly after takeoff was captured on a dash cam.
A TransAsia spokesman told news agency AFP: "We offered an amount of TW$14.9 million (£308,000) as compensation for each person who died in the accident. We hope to reach a settlement with the families.
"We can fully understand that it would be hard for the families to accept it immediately. Still we hope the representatives could take the proposal back and take it into consideration."
Investigators are still trying to establish what caused last week's crash, but initial reports from the black boxes found the plane's right engine had "flamed out" about two minutes after take-off.
Taiwan plane crash families receive airline money
TransAsia Airways has begun distributing $38,000 (£25,000) to the families of passengers killed in last week's crash in Taiwan for funeral costs.
A team of divers continue to search the river where the propeller-jet crashed for the three people still missing, using metal detectors to locate their watches.
At least 40 people died after the ATR 72 crashed into the Keelung River minutes after takeoff on Wednesday. Fifteen people were rescued.
TransAsia tests all pilots after Taiwanese plane crash
TransAsia Airways says all 71 of its ATR pilots have begun proficiency tests after one of the airline's planes crashed into a river in Taiwan, killing at least 36 people.
The airline said it has cancelled 90 flights in the next three days to accommodate the requirement by the Civil Aeronautics Administration that its pilots be retested.
Preliminary investigations indicate the pilots of Wednesday's tragic flight shut off a running engine on the ATR 72 after its other engine went idle, and aviation experts say the move could have been a mistake.
Fifteen people survived after the plane went into a river in the capital Taipei. The pilot, who had 4,900 hours of flying experience, has been hailed a hero for avoiding high rise buildings while the plane came down.
Both engines failed in Taiwan plane just seconds into flight
Both engines of a TransAsia plane which crashed into a river in Taiwan failed in the minutes before it fell from the sky, killing 35 people, authorities have revealed.
The jet was carrying 58 people when the engines stopped producing enough thrust for two minutes, forcing the pilots to try to turn one engine off and restart it.
Aviation Safety Council chief Thomas Wang said blackbox recordings recovered from the wreckage shows that problems began in the right engine just 37 seconds after takeoff, at 1,200 feet, when it suddenly entered "auto-feather" - meaning it reduces thrust to the propeller.
Problems then developed in the left engine. The crew tried reducing acceleration before attempting to restart it, but it failed to gain enough thrust.
The plane warned of stalling five times before the crash, Mr Wang added. The pilot announced a "flameout", which happens when fuel supply is interrupted, but there had not been one.
Eight people are still missing after the crash.
Taiwanese plane crash death toll 'rises to 35'
The death toll of the Taiwanese plane crash has risen to 35 as the search for passengers continues, the Associated Press reported.
Divers are searching a shallow river where a TransAsia Airways propjet crashed shortly after take-off from Taiwan's capital.
A group of relatives who had gathered on the riverbank in Taipei wailed in anguish as the bodies were brought out.
Helicopters helped to scan the river for eight people still missing. Fifteen survived the crash with injuries.
Taiwan plane crash pilots hailed as 'heroes'
The pilots of a plane which crashed into a river after clipping a bridge in Taiwan, killing at least 31 people, have been hailed as heroes by survivors and the city's mayor.
The TransAsia flight had not long departed from Taipei's Shongshan Airport when it ran into trouble. Its dramatic fall from the sky was caught on the dashboard cameras of passing motorists.
Read: Fresh footage of plane crash shows close call with buildings
The Mayor of Taipei, Ko Wen-je, praised the pilot for managing to narrowly avoid a number of apartment blocks, apparently aiming to land in the river which would give crew and passengers the best chance of survival.
In a tearful speech, he added: "He really tried everything he could."
Chris Lin, whose brother was among the crash's 15 survivors, also praised the pilot - named as 42-year-old Liao Chien-tsung.
The pilot's immediate reaction saved many people.
I was a pilot myself and I'm quite knowledgeable about the immediate reaction needed in this kind of situation.
We are very lucky.
Official investigations have been launched into what caused the crash.


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The District Department of Transportation (DDOT) will host its DC Circulator Semiannual Open House on October 6, 2015, to present information on current operations and performance and to solicit feedback from riders.
DDOT will provide information on the DC Circulator 2014 Transit Development Plan (TDP) and updates on future expansion.
Attendees will have the opportunity to provide input on the following areas of the program:
1. NoMa neighborhood route planning study
2. The next generation of DC Circulator buses
3. New DC Circulator website, RIDE DC app and bus tracking technology
4. Annual customer feedback survey
5. Possible bus stop consolidation on the Union Station to Navy Yard and Union Station to Georgetown routes
Thomson Elementary School
1200 L Street, NW
Washington, DC 20005
How to Get There
Capital Bikeshare:
12th & L St NW - 19 docks
11th & K St NW - 19 docks
Capital Bikeshare puts thousands of bikes at hundreds of stations at your fingertips. Annual, monthly and daily memberships are available. For a station map and more information, visit www.capitalbikeshare.com.
DC Circulator
Georgetown - Union Station route
Woodley Park – Adams Morgan – McPherson Square Metro
The DC Circulator runs every 10 minutes. Visit dccirculator.com for more information about routes and hours of operation.
Visit wmata.com/bus/timetables/ to view schedules and stop lists.
McPherson Square - Blue and Orange lines
Visit wmata.com/rail/ to view a system map and get more information.
Explore our interactive map to learn more about the transportation options available throughout DC.
The Honorable Minister Louis Farrakhan will convene the 20th Anniversary of the Million Man March under the theme: Justice or Else!
Get more event details at justiceorelse.com.
Taste of DC is a multi-day culinary and cultural event in the heart of the nation's capital. This expansive festival packs four blocks of Pennsylvania Avenue with area restaurants, great drinks, lively entertainment, engaging corporate partners, and fellow Washingtonians and visitors.
Get more details and tickets at tasteofdc.org.
Join the Coalition for Smarter Growth to discuss "Metro – The Next 20 Years" with Shyam Kannan, WMATA's Managing Director of Planning and an expert on Metro's role in shaping our region.
Mingle over brews from DC's Hellbender Brewing Company, local Virginia wines, and appetizers and meet hundreds of fellow smart growth supporters from all over the DC region. We'll also raffle off fun urbanist-themed prizes, including a 1-year Capital Bikeshare membership, and a copy of the cult-favorite game, Cards Against Urbanity.
Have a great night and know that you're supporting the Coalition for Smarter Growth's work to strengthen walkable and inclusive communities throughout the DC region! A significant portion of our annual budget comes from individuals like you. That means small donations from passionate urbanists, environmentalists, affordable housing activists, and other supporters are a really big deal each year.
Register for this event here.


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I'm killing the American dream with a trap
Like an axe, I'ma put it in a sack
Lyric Meaning
He's killing the American dream, he'll put it in a sack then put it at the back of a black cadillac (funeral car). He's could possibly be saying he's forgetting about the American dream as he's already living it perhaps?
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Greyhound Bus Lines
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Recent News from the Green Blog
California's Plastic Bag Ban: What's Not to Like?
Last month saw an enormous victory for environmentalists and proponents of sustainability as California became the first state in the union to enact a ban on single-use plastic grocery bags. The California law, slated to begin taking effect in 2015, has been hailed as bellwether legislation that is likely to spur other states to follow suit in passing laws that..


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Mortal Kombat PS Vita Mileena & Kitana Live Action Trailer
Other News: Mortal Kombat PS Vita: Live Action Mileena Trailer
Mileena & Kitana show off their stuff in a Live Action Trailer to promote Mortal Kombat on the PS Vita. Nice costumes and all, but I'd really like to see them fighting/acting like on Mortal Kombat: Legacy. Well here it is, enjoy!!
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Hi, I helm this righteous website, covering gaming, tech, entertainment and cosplay news. An island "Nerd" if you will, but I prefer to be described as "Tech Savvy and Prone to Awesomeness". Nice to meet you! P.S. Tell the security that "I'm the only thug in the building" o_O lulz
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Are PDA's Replacing Pillow Talk?
87% Of Workplace Professionals Take Their PDA to Bed, According to Work-Life Study
85% Say New Technology Means We're Never Off the Clock, but 84% Say it Means More Quality Time with Family and Friends
WHITE PLAINS, N.Y.--(BUSINESS WIRE)--Technology-addicted professionals just can't keep their hands off their personal digital assistants (PDA's), as new technology continues to transform the way we live and work. In a new work-life study from Sheraton Hotels & Resorts today, 85% of U.S. professionals surveyed said that because of modern technology, they feel compelled to be connected to work 24/7 and 81% say they work harder now than they did five years ago.
Most Folks Bring PDA's into the Bedroom; 35% Pick their PDA Over their Spouse
Just how technology-addicted is our society? A whopping 87% of professionals bring their PDA into the bedroom and, in a potentially related finding, more than one-third of folks surveyed (35%) say if forced to choose, they'd pick their PDA over their spouse! The vast majority of people (84%) also say they check their PDA's just before going to bed and as soon as they wake up, 85% say they sneak a peak at their PDA in the middle of the night and 80% say they check their email before morning coffee.
The Majority Love their PDA; Say New Technology Means More Time with Family
The Sheraton survey also revealed that, although we're working harder and new technology means we're never truly off the clock, it also enables us to spend more time away from the office and with family and friends. The days of being chained to a desk are over: 85% say that PDA's and cell phones allow them to spend more time out of the office and 79% feel they can be just as productive outside of the office as opposed to inside. Most people feel that technology gives them more quality time and flexibility with family and friends (84%), 77% say their PDA helps them enjoy life more and 62% love their PDA.
New Tech Makes Business Travel Seamless and Easier to Stay Connected to Home
Sheraton commissioned the work-life study to launch a new signature offering: "Link@Sheraton experienced with Microsoft," a unique lobby lounge that enables guests to stay connected, work and socialize through free Wi-Fi and Internet-enabled computer stations, video-chats, televisions and daily newspapers. The vast majority of professionals surveyed (88%) say new technology makes business travel easier or more seamless and 85% say new technology helps them stay better connected to family and friends when traveling on business.
The study was conducted by STUDYLOGIC LLC, who surveyed 6500 workplace professionals via phone with an individual income of $50K+, 2+ business trips per year and a Blackberry or mobile email device. Five countries were surveyed, including the U.S. (1,500 respondents), China, Australia, the United Kingdom and Germany.
Helping guests make connections at more than 400 hotels in 75 countries around the world, Sheraton is revitalizing its iconic brand while continuing its aggressive international expansion by adding 54 hotels and 20,000 guestrooms by 2009. Starwood Hotels & Resorts Worldwide, Inc. is one of the leading hotel and leisure companies in the world with approximately 900 properties in more than 100 countries and 155,000 employees at its owned and managed properties. Starwood Hotels is a fully integrated owner, operator and franchisor of hotels, resorts and residences with the following internationally renowned brands: St. Regis®, The Luxury Collection®, W®, Westin®, Le Méridien®, Sheraton®, Four Points® by Sheraton, and the recently launched AloftSM, and ElementSM. Starwood Hotels also owns Starwood Vacation Ownership, Inc., one of the premier developers and operators of high quality vacation interval ownership resorts. For more information, please visit www.starwoodhotels.com.


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The Monoprice Lineman's Test Set is a professional piece of equipment designed for telephone installers, repair technicians, and phone system maintenance personnel. This durable, line-powered device is full of features for testing existing and newly installed communications systems for proper functionality.
The bright red handset is both impact and water resistant. The rubber-covered keypad and large slide switches are designed for use with gloves, making this a convenient tool for line workers. The large, heavy-duty alligator clips support five different types of wire connections, including a bed-of-nails and an insulation piercing spike. The connecting cord is covered in cloth for durability and is equipped with a strain-relief connection at the RJ11X connector. A heavy-duty, spring-loaded belt clip allows the handset to be conveniently hung from a maintenance belt.
The tester has a three-position volume switch, which can provide up to 12 dB of volume gain. The ring mode can be set to send either DTMF tones or rotary dial pulses. A three-position mode switch allows the tester to be set to Monitor, Ring, or Talk modes:
- The Monitor mode provides a high-impedance coupling to the telephone line, allowing it to be monitored without interfering with existing conversations, data transmissions, or signalling.
- The Ring mode allows the tester to be on-hook with an electronic ringer connected to the telephone line. This provides a low-impedance coupling to the telephone line for monitoring of optional line identification tones.
- The Talk mode allows the technician to seize the line or initiate signalling. From this mode a number can be dialed using the keypad. Hanging up the line is as simple as switching to the Ring or Monitor mode.
The handset includes an automatic last-number redial function, which is capable of repeating up to 31 digits. It also features a number memory storage capability, allowing the technician to store up to 10 different numbers of up to 15 digits each, including the insertion of 3-second pauses for use with trunk lines. A pre-installed CR2032 lithium battery maintains the stored number memory and can be easily replaced by removing two screws and opening the handset.
To assist in line testing, the kit includes a 5-inch, 4-wire RJ11 test lead with spade lugs on the ends of each wire. Additionally, a 2.5mm headphone jack allows the technician to utilize a microphone/headset unit instead of the handset speaker and mic. When not in use, the headphone jack is protected from moisture with a rubber cover.
Support Files:
- User's Manual (Jul 15, 2011)
|Loop Limit
|4K ohms max at 48 VDC (5k meter - 26-gauge non-loaded) nominal 15 mA minimum loop current
|DC Resistor
|150 ohms typical t 80 mA current
|Monitor Impedance
|Low Impedance = 600 ohms typical at 1 KHz (Ring mode)
|High Impedance = 100K ohms typical at 1 KHz (Monitor mode)
|Rotary Dial Output
|Pulsing Rate
|10 +/- 0.5 pulses/sec
|Percent Break
|60% +/- 2%
|Interdigital Pause
|8.5 ms typical
|Leakage During Break
|> 50K ohms
|DTMF Output
|Tone Frequency Error
|+/- 1.5%
|Level of Tone Pair
|+ dBm max, -8 dBm min
|Low vs High Tone Difference
|-4 dBm max
|22.4 cm (8.8 inches)
|7.12 cm (2.8 inches)
|7.0 cm (2.75 inches)
|380 g (13.4 ounces) max
Included in the Box:
- 1x MCT-1900-RD Testing Handset
- 1x 61" RJ11/Alligator Clip Test Lead (attached to handset)
- 1x Spring-loaded Metal Belt Clip (attached to handset)
- 1x CR2032 Lithium Battery (installed inside handset)
- 1x 5" 4-wire RJ11 Test Lead with Spade Lugs
| rated by 7 user(s)
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BOOTS. Found these boots at the Zara sale and didn't think twice about buying them. Wish I could show you how awesome they look in motion with all the fringes dancing around my feet!
FRINGE BOOTS Zara.
LOVE. I'm not really into sports but in the spirit of the olympics, I just ordered myself the fashion equivalent of a supporter t-shirt. Team Philo all the way! ;) Which team are you on? Get your t-shirt here!
FIRENZE. OK, after the most nightmare like trip (litterally thought the plane was going to crash due to the insane storm in Zürich where I had my connecting flight), I FINALLY made it to Florence for the Luisa Via Roma event Firenze 4-ever!! So happy to be back again and of course reuniting with all my blogger friends. My boyfriend is flying in today so hopefully I'll be able to post some outfit pictures tomorrow!
Now, the first thing I did this morning was a quick visit to the Céline store where I bought something I've been looking for everywhere, any guesses on what's hiding in the bag? ;)
ZARA heels
LOVE. Sorry about the lack of posts these past days, I don't know why but the week before new years I'm always completely exhausted and out of inspiration. But come New Years Eve I'm usually back to my normal self, all excited about the upcoming year and making impossible new years resolutions like "start going to the gym 4 times a week" or "stop eating candy", you know, the kind of horrible promises you simply can't commit to!
Speaking of which, my post xmas "no shopping until next year" ban broke the minute I saw these awesome shoes on sale at Zara and it took me about 2 seconds to run to the cashier and buy them. Oh well, rules are meant to be broken, right? ;)


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People tend to check in during these times:
Add hours
A convenient place with great employees to obtain personal, small business and/or corporate financial advice and learn about a full range of financial products, including but not limited to: mortgages, loans, checking accounts, credit cards, and insurance.
Check ur stuff b4 u pull away
Ask for two dollar bills. Confuses the kids in the drive up windows at Burger King.


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Page 1
CFP1 Interacts with DNMT1 Independently of Association
with the Setd1 Histone H3K4 Methyltransferase Complexes
Jill Sergesketter Butler, Jeong-Heon Lee, and David G. Skalnik
CXXC finger protein 1 (CFP1) is a component of the Setd1A and Setd1B methyltransferase complexes, localizes to
euchromatic regions of the genome, and specifically binds unmethylated CpG dinucleotides in DNA. Murine
embryos lacking CFP1 exhibit peri-implantation lethality, a developmental time that correlates with global epi-
genetic reprogramming. CFP1-deficient embryonic stem (ES) cells exhibit a 70% reduction in global cytosine
methylation and a 60% decrease in maintenance DNA methyltransferase (DNMT1) activity. DNMT1 protein level
is reduced 50% in CFP1-deficient ES cells. Experiments were performed to investigate the role of CFP1 in regu-
lating maintenance cytosine methylation. Coimmunoprecipitation experiments reveal that endogenous DNMT1
and CFP1 interact in vivo. Protein regions required for the interaction between DNMT1 and CFP1 were mapped.
Amino acids 169–493 and 970–1617 of DNMT1 are each sufficient for interaction with CFP1. Three regions
spanning the CFP1 protein, amino acids 1–123, 103–367, and 361–656, are each sufficient for interaction with
DNMT1. Interestingly, a single-point mutation (C375A) within CFP1 that abolishes the interaction with the
DNMT1. This result indicates that CFP1 intersects the cytosine methylation machinery independently of its
association with the Setd1 complexes.
quence. Epigenetic modifications that facilitate changes in
chromatin structure and thus influence transcription include
posttranslational covalent modifications of histone tails and
cytosine methylation.
CXXC finger protein 1 (CFP1) is a unique DNA-binding
protein that contains a conserved CXXC domain both neces-
sary and sufficient for specific binding to unmethylated CpG
dinucleotides (Voo et al., 2000; Lee et al., 2001). CFP1 also
contains two plant homeodomain (PHD) fingers. Recently, a
global proteomic screen of Saccharomyces cerevisiae proteins
containing PHD fingers revealed that the PHD of Spp1, the
yeast homolog of CFP1, binds histone H3K4me2=3(Shi et al.,
2007). Additionally, the PHD within the NURF nucleosome
remodeling factor binds histone H3K4me3and targets the
NURF complex to Hox promoters during development to
activate transcription (Wysocka et al., 2006), thus demon-
strating a biological effect of PHD finger interaction with
methylated histone H3.
CFP1 is a component of the mammalian Setd1A (Lee and
Skalnik, 2005) and Setd1B (Lee et al., 2007) histone H3K4
pigenetics refers to heritable patterns of gene ex-
pression that occur without alteration of nucleotide se-
methyltransferase complexes, which are analogous to the
Set1=COMPASS complex in S. cerevisiae (Miller et al., 2001;
Nagy et al., 2002; Roguev et al., 2003). Spp1 is required for
Set1=COMPASS histone methyltransferase activity in Schi-
zosaccharomyces pombe, but only for appropriate histone
H3K4me3in S. cerevisiae (Roguev et al., 2003; Schneider et al.,
2005). While the mammalian Setd1A and Setd1B complexes
both localize to euchromatic regions of the genome, they
exhibit nonoverlapping subnuclear localization patterns (Lee
et al., 2007). This suggests that the Setd1A and Setd1B com-
plexes function at distinct genomic targets.
Mice lacking CFP1 exhibit peri-implantation lethality (4.5–
6.5 days postcoitus) (Carlone and Skalnik, 2001). CFP1 is ad-
ditionally required for postgastrulation development and
survival, because zebrafish embryos injected with antisense
morpholino oligonucleotides directed against CFP1 exhibit
defective primitive hematopoiesis and decreased survival
(Young et al., 2006). In addition, siRNA-mediated depletion of
CFP1 in human leukemia cell lines leads to defects in cell
proliferation and terminal myeloid differentiation (Young
and Skalnik, 2007). Lastly, CFP1-deficient murine embry-
onic stem (ES) cells are viable; however, they are unable to
differentiate upon removal of leukemia inhibitory factor
from the culture medium and exhibit a threefold increase in
Herman B Wells Center for Pediatric Research, Section of Pediatric Hematology=Oncology, Departments of Pediatrics and Biochemistry
and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana.
Volume 27, Number 10, 2008
ª Mary Ann Liebert, Inc.
Pp. 533–543
DOI: 10.1089=dna.2007.0714
Page 2
apoptosis under normal culture conditions (Carlone et al.,
CFP1-deficient ES cells exhibit a *43% increase in histone
H3K4me2and *28% reduction in histone H3K9me2under
normal conditions, and an approximately fourfold induction
of histone H3K4me3upon induction of differentiation (Lee
and Skalnik, 2005). CFP1-deficient ES cells also exhibit a 70%
deficiency in global cytosine methylation that affects single-
copy genes, imprinted loci, and repetitive elements (Carlone
et al., 2005). Taken together, these data indicate that ES cells
lacking CFP1 contain reduced heterochromatin. A 60% re-
duction in maintenance DNA methyltransferase (DNMT1)
activity appears to contribute to the global cytosine methyla-
tion deficiency, because de novo DNMT activity is not de-
creased in ES cells lacking CFP1 (Carlone et al., 2005). DNMT1
protein expression is reduced 50% in the absence of CFP1
(Carlone et al., 2005) due to decreased DNMT1 protein sta-
bility and reduced translation (Jill Butler, unpublished data).
Recent reports reveal intricate interrelationships linking
cytosine methylation and histone modifications, thus pro-
viding a unifying framework for the control of chromatin
structure and gene regulation (Burgers et al., 2002). For ex-
ample, DNMT proteins associate with histone deacetylase
(HDAC) complexes (Fuks et al., 2000, 2001; Robertson et al.,
2000); cytosine methylation in Neurospora is dependent on
methylation of histone H3 and the presence of heterochro-
matin protein 1 (HP1) (Freitag et al., 2004); human HP1 re-
cruits DNMT1 to methylate DNA and silence gene expression
(Smallwood et al., 2007), and inhibition of HDAC activity by
trichostatin A results in a loss of cytosine methylation (Selker,
1998; Tamaru and Selker, 2001; Tamaru et al., 2003). Further,
the chromatin remodeling protein DDM1 in Arabidopsis and
the related factor LSH in mammals are required for normal
cytosine methylation (Jeddeloh et al., 1998, 1999; Dennis et al.,
2001). Disruption of the Suv39h1 histone H3K9 methyl-
transferase gene in murine ES cells leads to altered locali-
zation of DNMT3b and decreased cytosine methylation at
peri-centric satellite repeats (Lehnertz et al., 2003), and loss of
DNMT1 leads to perturbations in the histone code consistent
with reduced heterochromatin (Espada et al., 2004). Hence,
DNA methylation and covalent histone modifications are in-
tegrated and mutually reinforcing mechanisms to establish
and maintain heterochromatin.
However, the molecular mechanisms linking cytosine
methylation and histone modifications are not well under-
stood. Interestingly, organisms that lack cytosine methyla-
tion, such as Caenorhabditis elegans and S. cerevisiae, contain
CFP1 orthologs that lack the CXXC DNA-binding domain
(Voo et al., 2000). This suggests that CFP1 served an ances-
tral role in the regulation of covalent histone modifications,
and acquired DNA-binding capacity in organisms that uti-
lize cytosine methylation as an additional epigenetic modi-
fication. The influence of CFP1 on both cytosine methylation
and histone methylation suggests a role in mediating cross
talk between these epigenetic modifications.
Studies were performed to gain insight into the molecular
mechanism of how CFP1 affects cytosine methylation. The re-
sults presented in this report demonstrate that CFP1 and
DNMT1 physically interact. The minimal regions sufficient for
the interaction between DNMT1 and CFP1 were mapped and
include conserved domains involved in chromatin targeting.
Experiments were also performed to map the minimal region
required for CFP1 interaction with Setd1A and Setd1B. In-
terestingly, CFP1 interaction with Setd1A or Setd1B is not re-
quired for its interaction with DNMT1, strongly suggesting
cytosine methylation.
Materials and Methods
Cell culture and transient transfections
Human embryonic kidney (HEK-293) cells were cultured
as previously described (Lee and Skalnik, 2002; Carlone et al.,
2005). Transient cotransfections in HEK-293cells were per-
formed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA)
per the manufacturer's protocol. Briefly, cells were grown to
90–95% confluence as a monolayer in 10cm tissue culture
dishes. Ten micrograms of plasmid DNA was added to Li-
pofectamine 2000 reagent in OPTI-MEM serum-free medium
(Invitrogen). The mixture was added drop-wise to the cells,
which were then incubated for *40h at 378C with 5% CO2.
Construction of DNMT1 and CFP1 expression vectors
The human DNMT1 cDNA (generously provided by Dr.
Sriharsa Pradhan; New England Biolabs, Ipswich, MA) or
human CFP1 cDNA was ligated to the pcDNA3-FLAG vec-
tor that encodes an amino terminal FLAG epitope, as pre-
viously described (Lee et al., 2000). The CFP1 cDNA was
ligated to the pcDNA3-Myc vector that encodes an amino-
terminal Myc epitope. Constructs carrying truncations of the
DNMT1 or CFP1 cDNAs were generated using a combina-
tion of polymerase chain reaction (PCR) and restriction en-
zyme digestion methods, and then subcloned into the
pcDNA3-FLAG or pcDNA3-Myc expression vectors. Primer
sequences for PCR amplification are available upon request.
An exogenous nuclear localization signal (NLS) was made by
annealing complementary oligonucleotides corresponding to
the endogenous DNMT1 NLS that extends from amino acids
193–213. These oligonucleotides were designed to create a 50
EcoRI restriction enzyme site and a 30ClaI restriction enzyme
site when annealed. DNMT1 N-terminal deletion constructs
lacking the endogenous NLS were subcloned downstream of
the exogenous NLS in the pcDNA3-FLAG vector using the
EcoRI and ClaI restriction enzyme sites. Site-directed muta-
genesis was carried out using a QuikChange site-directed
mutagenesis kit (Stratagene, La Jolla, CA) per the manufac-
turer's protocol. The sequences of the oligonucleotide prim-
ers used to generate amino acid substitutions within the
human CFP1 cDNA are as follows: C375A, 50-cctgcgtcactgcc
ccaggcgctggggcccggctgtgtg-30; Y390A, C391A, S392A, 50-
ccgcccagcccagctccaaggcagccgccgatgactgtggcatgaagct-30; and
C580A, 50-gagctcacgggtgacttcgcccgcctgcccaagcgccag-30. The
nucleotide substitutions within the CFP1 primer sequences
are underlined. The nucleotide sequences of all constructs
were confirmed by automated DNA sequencing.
Coimmunoprecipitation and western blot analysis
Nuclear extracts were prepared from HEK-293cells tran-
siently expressing Myc and FLAG epitope-tagged proteins.
Cells were washed with 1? PBS, and then with hypotonic
acid [pH 7.9 at 48C], 1.5mM MgCl2, and 10mM KCl). Cells
were then swollen on ice for 10min in hypotonic buffer before
Page 3
being lysed by Dounce homogenization 10 times. Nuclei were
pelleted by centrifugation at 48C and resuspended in lysis
buffer (20mM Tris HCl [pH 7.4], 400mM NaCl, 25% glycerol,
5mM ethylenediaminetetraacetic acid, 0.1% NP-40, 1mM
dithiothreitol, and 3% protease inhibitor cocktail [Sigma, St.
Louis, MO]). Nuclei were lysed by Dounce homogenization
30 times on ice, and debris was pelleted by centrifugation at
Either anti-Myc–conjugated agarose (Sigma) or FLAG M2–
conjugated agarose (Sigma) was incubated with soluble
nuclear extracts for 3h at 48C. Alternatively, the soluble
nuclear extracts were precleared with protein G agarose
(Millipore, Billerica, MA), and then incubated with CFP1 an-
tiserum (Lee et al., 2007) or a custom DNMT1 antiserum di-
the murine cDNA for 1h at 48C, followed by incubation with
with buffer containing 300mM NaCl, 0.1% NP-40, and then
on PAGEr-Goldprecast4–12%or4–20%Tris-glycinegradient
gels (Lonza Group, Basel, Switzerland) by sodium dodecyl
sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and
transferred to nitrocellulose membranes (Amersham, GE
Healthcare, Piscataway, NJ). Western blot analysis was per-
formed using primary antisera directed against the Myc epi-
tope (Santa Cruz Biotechnology, Santa Cruz, CA), FLAG
epitope (Sigma), or the Setd1 complex components Setd1A
(Lee et al., 2007), Setd1B (Lee et al., 2007), Wdr5 (Lee et al.,
2007), Wdr82 (Lee et al., 2007), Ash2 (Bethyl Laboratories,
Montgomery, TX), and Rbbp5 (Bethyl Laboratories), followed
by horseradish peroxidase–conjugated secondary antibodies.
Proteins were detected using an ECL detection kit (Amer-
sham, GE Healthcare, Montgomery, TX).
CFP1 and DNMT1 interact in vivo
To determine if DNMT1 and CFP1 interact in vivo, full-
length Myc-CFP1 and full-length FLAG-DNMT1 were tran-
siently expressed in HEK-293cells, and Myc-CFP1 was
expressing Myc-CFP1 (full length) and FLAG-DNMT1 (full length), as indicated above the lanes, and were subjected to
immunoprecipitation using anti-Myc–conjugated agarose. Western blot analysis with anti-FLAG antiserum was used to detect
FLAG-DNMT1. The membrane was re-probed with anti-Myc antiserum to verify Myc-CFP1 protein expression and Myc
immunoprecipitation efficiency. Transfection and immunoprecipitation of the empty vector serves as a negative control for the
Myc immunoprecipitation. (B) Nuclear extracts were prepared from HEK-293 cells transiently expressing Myc-CFP1 (full
length) and FLAG-DNMT1 (full length), as indicated above each lane,and were subjected toimmunoprecipitation using FLAG
M2–conjugated agarose. Western blot analysis with anti-Myc antiserum was used to detect Myc-CFP1. The membrane was re-
probed with anti-FLAG antiserum to verify FLAG-DNMT1 protein expression and FLAG immunoprecipitation efficiency.
Transfection and immunoprecipitation of the empty vector serves as a negative control for the FLAG immunoprecipitation.
(C) Endogenous CFP1 was immunoprecipitated from HEK-293 nuclear extracts using CFP1 antiserum and protein G agarose.
Western blot analysis was performed using anti-DNMT1 antiserum. The signal for DNMT1 is marked with an arrow. Im-
munoprecipitation reactions containing control serum (IgG control) or lacking primary antibody (No Ab) serve as negative
controls for the CFP1 immunoprecipitation. (D) Endogenous DNMT1 wasimmunoprecipitated from HEK-293 nuclear extracts
using antiserum against DNMT1 and protein G agarose. Western blot analysis was performed using anti-CFP1 antiserum. The
signal for CFP1 is marked with an arrow. An immunoprecipitation reaction lacking primary antibody (No Ab) serves as a
negative control for the DNMT1 immunoprecipitation.
CFP1 and DNMT1 physically interact in vivo. (A) Nuclear extracts were prepared from HEK-293 cells transiently
Page 4
immunoprecipitated from nuclear extracts using anti-Myc–
conjugated agarose. The immunoprecipitated material was
subjected to western blot analysis using antiserum against the
FLAG epitope. These experiments demonstrate that FLAG-
DNMT1 coimmunoprecipitates with Myc-CFP1 (Fig. 1A).
Empty vector was cotransfected individually and with each
construct to serve as a negative control for Myc immunopre-
cipitation reactions. The reciprocal experiment demonstrated
that Myc-CFP1 coimmunoprecipitates with FLAG-DNMT1
upon immunoprecipitation with FLAG M2–conjugated aga-
rose, thus confirming the interaction (Fig. 1B). These re-
sults indicate that full length CFP1 and DNMT1 interact
in vivo.
To examine whether CFP1 and DNMT1 interact at phys-
iologic concentrations in vivo, endogenous CFP1 was im-
munoprecipitated from HEK-293 nuclear extracts using
CFP1 antiserum. The immunoprecipitated material was
subjected to western blot analysis using antiserum against
in HEK-293 nuclear extracts (Fig. 1C). Addition of protein G
agarose to nuclear extracts without antibody or with control
serum served as negative controls. The reciprocal experiment
the conserved domains within DNMT1. DMAP, DNMT1-associated protein 1 binding region; PCNA, proliferating cell nuclear
antigen–binding region; NLS, nuclear localization signal; TS, region required to target DNMT1 to chromatin independently of
the replication fork; CXXC, conserved cysteine-rich DNA-binding domain; BAH, bromo-adjacent homology domain. The
amino acid position of each domain is denoted below the diagram. (B) A series of FLAG-DNMT1 C-terminal deletion mutants
encoding amino acids 1–650, 1–489, 1–173, and 169–493 were coexpressed with Myc-CFP1 (aa 1–367) in HEK-293 cells. Nuclear
extracts were prepared and subjected to Myc immunoprecipitation, followed by western blot analysis using anti-FLAG
antiserum. The FLAG-DNMT1 truncation mutants analyzed for interaction are denoted above each lane. The membranes were
re-probed withMyc antiserum to verifyMyc-CFP1 (aa 1–367) protein expression and Myc immunoprecipitation efficiency. The
empty vector was transfected alone or with Myc-CFP1 (aa 1–367) into HEK-293 cells to serve as negative controls for the Myc
tested for interaction with Myc-CFP1 (aa 1–367). FLAG-DNMT1 proteins were immunoprecipitated with Myc-conjugated
denoted above each lane. The membranes were re-probed with Myc antiserum to verify Myc-CFP1 (aa 1–367) protein ex-
pression and Myc immunoprecipitation efficiency. The empty vector was cotransfected with FLAG-DNMT1 (aa 637–1102) or
FLAG-DNMT1 (aa 970–1617) to serve as negative controls for the Myc immunoprecipitation.
Two independent domains of DNMT1 are each sufficient for interaction with CFP1. (A) A schematic representation of
Page 5
was performed using DNMT1 antiserum to coimmunopre-
cipitate CFP1 from HEK-293 nuclear extracts. Western blot
analysis using CFP1 antiserum confirmed the interaction
with endogenous DNMT1 (Fig. 1D). These results demon-
strate a physical interaction between endogenous DNMT1
and CFP1.
Independent domains within the amino and carboxyl
termini of DNMT1 are each sufficient for interaction
with CFP1
The amino terminus of DNMT1 interacts with a number of
chromatin-associated proteins, such as DNMT3a and 3b (Kim
et al., 2002), HDAC1 (Fuks et al., 2000; Robertson et al., 2000)
and HDAC2 (Rountree et al., 2000), the methyl CpG-binding
proteins, MeCP2 (Kimura and Shiota, 2003) and MBD2 and
MBD3 (Tatematsu et al., 2000), HP1 (Fuks et al., 2003; Small-
wood et al., 2007), and the histone H3K9 methyltransferases,
SUV39H1 (Fuks et al., 2003) and G9a (Esteve et al., 2006). A
diagram illustrating conserved domains within DNMT1 is
shown in Figure 2A. To further characterize the minimal re-
gion of DNMT1 required for interaction with CFP1, FLAG-
tagged,truncatedDNMT1protein fragmentsweretransiently
expressed in HEK-293cells along with Myc-CFP1 (aa 1–367),
followed by Myc immunoprecipitation. The C-terminal
truncated proteins, FLAG-DNMT1 (aa 1–650) and (aa 1–489),
interact with Myc-CFP1 (Fig. 2B). Analysis of the N-terminus
of DNMT1 revealed that the first 173 amino acids, encoding
the DNMT1-associated protein 1 (DMAP) and proliferating
cell nuclear antigen (PCNA)–binding regions, are not suffi-
cient for interaction with Myc-CFP1 (Fig. 2B). The FLAG-
large portion of the targeting sequence (TS) that directs
DNMT1 to late-replicating heterochromatin (Easwaran et al.,
2004). This portion of DNMT1 is sufficient for the interaction
with Myc-CFP1 (Fig. 2B).
It was also necessary to analyze the C-terminus of DNMT1
for possible interaction with CFP1. The highly conserved C-
terminus of DNMT1 has previously been found to interact
with chaperone 23 (p23) (Zhang and Verdine, 1996), the tu-
mor suppressor p53 (Esteve et al., 2005), and the histone
H3K27 methyltransferase, EZH2 (Vire et al., 2006). The highly
conserved C-terminal catalytic domain contained within the
FLAG-DNMT1 (aa 970–1617) protein fragment is sufficient to
interact with Myc-CFP1 (aa 1–367) (Fig. 2C). Bromo-adjacent
homology (BAH) domains are found in transcriptional regu-
latoryproteinsandoftenmediateprotein–protein interactions
(Callebaut et al., 1999). However, the BAH domains of
DNMT1 are not sufficient for interaction with Myc-CFP1 (Fig.
2C), as the FLAG-DNMT1 (aa 637–1102) protein fragment
fails to interact with Myc-CFP1 (aa 1–367). Lastly, central re-
gions of DNMT1, including amino acids 392–661, 169–330,
and 169–392, failed to interact with CFP1 (aa 1–367) (data not
shown). These results demonstrate a complex pattern of in-
teractions, in which either of two nonoverlapping domains of
DNMT1 is sufficient for interaction with CFP1.
CFP1 interacts with DNMT1 through several
conserved domains
CFP1 contains multiple highly conserved domains that
contribute to CFP1 subcellular localization and function (Voo
et al., 2000). For example, the acidic, basic, and coiled-coil
domains are necessary and sufficient to target CFP1 to the
nuclear matrix (Lee and Skalnik, 2002), while the CXXC do-
main is required for DNA binding (Lee et al., 2001). To de-
termine if any ofthese conserved domains arerequired for the
interaction with DNMT1, various CFP1 protein fragments
were expressed in HEK-293cells and tested for interaction
with DNMT1. A schematic diagram representing the organi-
zation of conserved domains within the CFP1 protein is
shown in Figure 3A. Truncations encoding Myc-tagged C-
terminal deletion mutants of CFP1 were transiently expressed
in HEK-293cells along with FLAG-DNMT1 (aa 1–489).
Immunoprecipitation was carried out using FLAG M2-
conjugated agarose. Western blots probed with anti-Myc
antiserum demonstrate that Myc-CFP1 (aa 1–123), which
contains the PHD1 domain, is sufficient for interaction with
analyzed for interaction with DNMT1. Western blot analysis
demonstrates thatMyc-CFP1(aa103–367),which containsthe
CXXC, acidic, and basic domains, is sufficient for interaction
with FLAG-DNMT1 (Fig. 3C). However, Myc-CFP1 (aa 213–
367), which lacks the CXXC domain, fails to interact with
FLAG-DNMT1, indicating that the acidic and basic domains
of CFP1 are not sufficient for interaction with DNMT1 (Fig.
3C). Taken together, these results indicate that multiple re-
gions within CFP1 are sufficient, but no individual domain is
necessary, for interaction with DNMT1.
The carboxyl terminus of CFP1 mediates
the interaction with the Setd1A and Setd1B
histone methyltransferase complexes
CFP1 is a component of the Setd1A and Setd1B histone
H3K4 methyltransferase complexes (Lee and Skalnik, 2005;
Lee et al., 2007). The Setd1A and Setd1B complex–interacting
region within CFP1 was mapped using truncation analysis to
determine whether CFP1 interacts with the cytosine methyl-
ation machinery while associated with the Setd1 complexes.
A series of FLAG-epitope–tagged CFP1 truncation mutants
were expressed in HEK-293cells. Nuclear extracts were pre-
pared and subjected to immunoprecipitation using FLAG
M2–conjugated agarose. Western blot analysis was then per-
formed using antisera directed against components of the
Setd1A and Setd1B histone methyltransferase complexes—
Setd1A, Setd1B, Ash2, Rbbp5, Wdr5, and Wdr82. The Setd1A
and Setd1B complexes consist of identical components with
exception of the catalytic (Setd1) subunits (Lee and Skalnik,
2005; Lee et al., 2007). Coimmunoprecipitation studies re-
vealed that a Setd1-interacting domain (SID) lies between the
conserved basic and coiled-coil domains of CFP1 (Fig. 4A, B).
The SID of CFP1 is required for CFP1 interaction with the
Setd1A and Setd1B complexes, because the FLAG-CFP1 (aa
423–656) mutant that lacks this region fails to interact with
any components of the Setd1A or Setd1B complexes (Fig. 4B).
Further, the C-terminus of CFP1, which contains the PHD2
domain, is also required for interaction with the Setd1A and
that contains the SID, but lacks the C-terminus, also fails to
interact with any Setd1A or Setd1B complex components
(Fig. 4). Finally, FLAG-CFP1 (aa 360–597) fails to interact
with the Setd1 complexes, demonstrating that the SID and
PHD2 domains are not sufficient for this interaction (Fig. 4).


text:
Capital Markets
Company Overview of Assante Corp.
BOARD MEMBERS AFFILIATED WITH Eric L. Stefanson FCA*
Chief Financial Officer - Finance &Planning Assante Canada and Managing Director - Finance &Planning Assante Canada
*Data is at least as current as the most recent Definitive Proxy.
MOST CONNECTED PEOPLE TO Eric L. Stefanson FCA
|H. Sanford Riley
|168 Relationships
|James Francis Dinning
|136 Relationships
|Charles Wayne Fischer
|69 Relationships
|Edward S. Kennedy
|50 Relationships
|James K. Gray
|41 Relationships
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