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Nonhepatic origin of notothenioid antifreeze reveals
pancreatic synthesis as common mechanism in polar
fish freezing avoidance
Chi-Hing C. Cheng*†, Paul A. Cziko*, and Clive W. Evans‡
*Department of Animal Biology, University of Illinois, Urbana, IL 61801; and‡Molecular Genetics and Development, School of Biological Sciences,
University of Auckland, Auckland, New Zealand
Communicated by George N. Somero, Stanford University, Pacific Grove, CA, May 9, 2006 (received for review March 30, 2006)
Phylogenetically diverse polar and subpolar marine teleost fishes
have evolved antifreeze proteins (AFPs) or antifreeze glycopro-
teins (AFGPs) to avoid inoculative freezing by internalized ice. For
over three decades since the first fish antifreeze (AF) protein was
discovered, many studies of teleost freezing avoidance showed
hepatic AF synthesis and distribution within the circulation as
pivotal in preventing the blood, and therefore the fish, from
freezing. We have uncovered an important twist to this long-held
paradigm: the complete absence of liver synthesis of AFGPs in any
life stage of the Antarctic notothenioids, indicating that the liver
plays no role in the freezing avoidance in these fishes. Instead, we
found the exocrine pancreas to be the major site of AFGP synthesis
and secretion in all life stages, and that pancreatic AFGPs enter the
intestinal lumen via the pancreatic duct to prevent ingested ice
from nucleating the hyposmotic intestinal fluids. AFGPs appear to
remain undegraded in the intestinal milieu, and the composition
and relative abundance of intestinal AFGP isoforms are nearly
identical to serum AFGPs. Thus, the reabsorption of intact pancre-
as-derived intestinal AFGPs, and not the liver, is the likely source of
circulatory AFGPs in notothenioid fishes. We examined diverse
northern fish taxa and Antarctic eelpouts with hepatic synthesis of
bloodborne AF and found that they also express secreted pancre-
atic AF of their respective types. The evolutionary convergence of
this functional physiology underscores the hitherto largely unrec-
ognized importance of intestinal freezing prevention in polar
teleost freezing avoidance, especially in the chronically icy Antarc-
tic waters.
antifreeze glycoprotein-null liver ? antifreeze paradigm shift ? evolutionary
adaptation ? intestinal freeze avoidance ? functional convergence
(20–35 mg?ml) of protein antifreeze (AF) (a family of AF glyco-
proteins; AFGPs) in their blood and other body fluids (4, 5). The
synthesis of blood AFGPs in Antarctic notothenioids has histori-
cally been attributed to the liver (6, 7), because the vertebrate liver
is well known as the major source of secreted plasma proteins (8,
9), and thus there were no a priori reasons to invoke a different
source for the abundant plasma AFGPs. Also contributing to the
prevailing notion of universal hepatic AF synthesis is the readily
demonstrable liver expression of AF mRNA by Northern blots
and?or cDNA cloning in all other AF protein (AFP)-bearing fish
AFGP-bearing polar cod (15). However, definitive verification of
liver biosynthesis of AFGPs in Antarctic notothenioids has been
lacking. Early radioactive-tracer investigations of notothenioid
AFGP biosynthesis could not rule out a nonhepatic synthesis site,
because the appearance of labeled AFGPs and non-AFGP plasma
proteins in the blood was drastically asynchronous (AFGPs lagged
nototheniid Notothenia coriiceps showed AFGP mRNA hybridiza-
tion, but an unusually large amount (50 ?g) of polyA? RNA was
he predominant endemic Antarctic marine teleost group, the
notothenioid fishes (1), inhabit the world's coldest and iciest
required (16), contradictory to liver being a strong expression site.
If there is little or no hepatic AFGP biosynthesis, the tissue origin
of the abundant bloodborne AFGPs in Antarctic notothenioids
returns as an unsolved fundamental question ?30 years after the
discovery of the protein (5). In our prior elucidations of the
evolution of the notothenioid AFGP gene from a pancreatic
TLP cDNAs were obtained from exocrine pancreas RNA, indicat-
ing that exocrine pancreas is an AFGP expression site (17, 18). In
this comprehensive study, we confirm that the exocrine pancreas is
the major AFGP synthesis site in Antarctic notothenioid fishes
from hatching through adulthood, whereas the liver is AFGP-
expression null in all life stages. We show that pancreatic AFGPs
are secreted into the intestine, and, with additional AFGP contri-
bution from the anterior portion of the stomach (the only other
icy Antarctic waters. The apparently undegraded intestinal AFGPs
raise the possibility that plasma AFGPs in the notothenioids are
derived from reabsorption of these intact macromolecules, which
despite the absence of liver AF synthesis and secretion. In addition,
we examine diverse AF-bearing species from both north- and
south-polar and subpolar regions and confirm that they have
which attests to intestinal freezing prevention as a vital and integral
component of the repertoire of teleost freeze-avoidance strategies.
AFGP mRNA Expression in Adult Notothenioid Tissues. To determine
the tissue site or sites of AFGP synthesis in adult notothenioids,
Northern blots of total RNA from different tissues were hybridized
to an AFGP gene (coding region only) probe (Fig. 1). Like the
phyletically basal AFGP-null New Zealand notothenioid Bovichtus
each of the five endemic Antarctic notothenioid families (Noto-
theniidae, Artedidraconidae, Harpagiferidae, Bathydraconidae,
and Channichthyidae; detailed species information in Table 1,
showed no hybridization, as opposed to the intense hybridization in
pancreatic RNA sampled from the Antarctic nototheniid Dissos-
tichus mawsoni included in the blot (Fig. 1A Upper). As a control,
Conflict of interest statement: No conflicts declared.
Abbreviations: AF, antifreeze; AFGP, antifreeze glycoprotein; AFP, antifreeze protein; GI,
gastrointestinal; IF, intestinal fluid; PF, pancreatic fluid; TLP, trypsinogen-like protease.
Data deposition: The 26 sequences reported in this paper have been deposited in the
GenBank database (accession nos. DQ062435–DQ062459 and DQ394083). Details listed in
Table 4, which is published as supporting information on the PNAS web site.
†To whom correspondence should be addressed at: Department of Animal Biology,
515 Morrill Hall, 505 South Goodwin Avenue, University of Illinois, Urbana, IL 61801.
E-mail: firstname.lastname@example.com.
© 2006 by The National Academy of Sciences of the USA
www.pnas.org?cgi?doi?10.1073?pnas.0603796103PNAS ?
July 5, 2006 ?
vol. 103 ?
no. 27 ?
Page 2
(Fig. 1A Lower).
In contrast to liver RNA, Northern blot of RNA from pancreatic
tissues of species representing the five endemic Antarctic notothe-
nioid families showed strong AFGP mRNA expression (Fig. 1B
Upper). Because adult teleost exocrine pancreas is commonly
diffuse and scattered beneath the surface or between layers of
mesenteric (peritoneal or serosal) and fatty tissues associated with
abdominal organs and the biliary and pancreatic ducts (19, 20), we
confirmed the presence of pancreatic mRNA in our pancreatic
tissue isolates based on the positive hybridization with a probe
derived from a D. mawsoni pancreatic trypsinogen cDNA (Fig. 1B
Lower). Lower gastrointestinal (GI) tract components (pyloric ceca
and intestine) with surface mesentery removed showed no AFGP
expression (Fig. 1B Upper). The sizes of pancreatic AFGP mRNAs
ranged from ?1.35 kb to ?9.5 kb (Fig. 1B Upper), reflecting the
chimeric AFGP-TLP genes found in extant Antarctic notothenioid
Antarctic bathydraconid Gymnodraco acuticeps identified the an-
terior portion of the stomach immediately next to the esophagus-
stomach junction as the only other strong expression site of AFGP
mRNA in adults (Fig. 1C).
AFGP Expression in Larval Notothenioid Fish. Immunodetection of
mature AFGPs using polyclonal rabbit anti-AFGP antibodies and
fluorescently labeled secondary goat anti-rabbit IgG in tissue
sections of a 1-day-old G. acuticeps hatchling (Fig. 2 A–D and G)
and a 4-month-old larva (Fig. 2 E, F, and H) showed no immuno-
reactive AFGPs in the liver cells of either age, but both ages
revealed a high degree of AFGP expression in pancreatic acinar
cells, particularly within apical zymogen granules. Pancreatic
AFGP-positive and hepatic AFGP-null expression is therefore not
an adult-only condition, but occurs at hatching and in the early life
stages of notothenioid fishes.
Osmolarity and AF Activity of GI Fluids. The strong AFGP mRNA
expression in the adult exocrine pancreas and anterior stomach
(Fig. 1) is associated with the presence of significant AF activity
(measured as thermal hysteresis, the difference between melting
2, which is published as supporting information on the PNAS web
site), indicating that transcripts are translated into secreted pro-
teins. The stomach fluid is isosmotic or moderately hyposmotic
Fig. 1.
the five Antarctic notothenioid families (lanes 2–5) and the basal AFGP-null New Zealand species Bv (B. variegatus) (lane 6), versus strong pancreatic expression
liver samples (Lower). (B) AFGP expression in pancreatic tissue isolates from selected species from all five Antarctic notothenioid families and no expression in
nonpancreatic GI components (Upper). Location of pancreatic tissue isolates: st, stomach; pc, pyloric ceca; gb, gall bladder; gi, GI locations; ant, anterior; post,
posterior. Hybridization of pancreatic RNA samples to a pancreatic trypsinogen probe verifies presence of exocrine pancreas RNA (Lower). (C) AFGP expression
in anterior stomach (lanes 1 and 2) and absence of expression in all other tissues from Ga (G. acuticeps). (Detailed species information is in Table 1.)
Northern blot analysis of AFGP mRNA expression in adult notothenioid fish tissues. (A) Absence of liver AFGP expression in five species representing
Fig. 2.
acuticeps larvae. (A–D) One-day-old larva. (A) Phase-contrast image showing
DAPI-stained image illustrating the position of nuclei (blue). (C) Immunola-
DAPI, and immunofluorescence). The inset (3? magnification) shows AFGP
localization in some (open arrow) but not all zymogen granules (filled arrow)
of the liver. Immunostained AFGP is lacking. (F) Composite image of the
granules of acinar cells (open arrow). (G) Image of a 1-day-old hatchling. (H)
Image of a 4-month-old larva.
Immunodetection of AFGP expression in Antarctic notothenioid G.
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www.pnas.org?cgi?doi?10.1073?pnas.0603796103 Cheng et al.
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(1,039?21 to 772?151 mOsm) to seawater (1,025 mOsm), reflect-
dilution by the incipient digestion in the stomach. Intestinal fluid
(IF) is significantly hyposmotic (603?65 to 748?27 mOsm) to
seawater, reflecting dilution by GI secretions, late-stage digestion,
and intestinal salt absorption (21). The presence of AFGPs in the
stomach fluid and IF depresses their freezing points to below that
neither fluid risks inoculative freezing by ingested ice. Thermal
indicating the presence of abundant AF. Pancreatic fluid (PF) was
only obtained from the large nototheniid D. mawsoni in which the
pancreatic duct (distinct from the bile duct) visibly distends into a
small reservoir at its base above its junction with the intestine. D.
mawsoni PF displays a large thermal hystersis (0.94?0.38°C),
indicative of the presence of substantial AFGPs before secretion
into the intestinal lumen.
of size isoforms (15, 22), and the profiles of electrophoretically
resolved AFGPs purified from the PF and IF of one D. mawsoni
specimen show they comprise the full complement of isoforms
similar to serum AFGPs of the same specimen in terms of size
IF AFGP profiles support the pancreas as a major source of
intestinal AFGPs, with the input of stomach AFGPs to the IF
contributing to the observed IF profile (Fig. 4A). Intestinal AFGP
from the anterior and posterior half of the intestine respectively
(Fig. 4A). Profiles of IF and serum AFGPs are nearly identical,
suggesting the possibility that the latter may be derived from the
former via reabsorption. For species of the other four Antarctic
notothenioid families, which are too small for PF sampling, we
cloned and sequenced partial pancreatic AFGP cDNAs from a
representative member of each family. All pancreatic cDNAs
encode AFGPs that share a conserved signal peptide sequence
(Fig. 6, which is published as supporting information on the PNAS
web site), indicating their translation into secreted proteins. Pan-
creatic AFGP expression and secretion therefore occurs through-
out members of all the Antarctic notothenioid families.
Pancreatic AF Expression in Nonnotothenioid AF-Bearing Fishes. The
need for protecting hyposmotic IF from freezing also applies to
other polar fishes; thus, we examined diverse species (eleven) from
both north- and south-polar (and subpolar) regions bearing all
other known types of AFs (species and AF information in Table 1).
Northern blots of RNA from fish bearing each type of AF hybrid-
ized with the corresponding AF cDNA (or gene) probe showed
pancreatic AF expression (Fig. 5 Upper) in addition to liver expres-
sion (Fig. 5 Upper and refs. 10–15). The pancreatic AFP mRNA of
type I, II, III, and IV AFP-bearing species corresponds in size to a
liver AFP mRNA (Fig. 5 A–D Upper), indicating expression of a
type III AFP mRNA in zoarcoid fishes (pouts and wolffish) (Fig.
5C), likely due to the tissue heterogeneity of the pancreatic isolates
and?or seasonal variation of synthesis in the northern hemisphere
species (23) (Atlantic ocean pout Macrozoarces americanus and
spotted wolffish Anarhichas minor sampled in late spring). Strong
pancreatic AFGP mRNA expression is seen in the three northern
kb, with predominant transcripts at ?2 kb for the saffron cod
Eleginus gracilis and ?3 kb and 9 kb for the high-latitude polar cod
Boreogadus saida and the icecod Arctogadus glacialis (Fig. 5E
Upper), reflecting AFGP multigene families and the large polypro-
tein structure of the transcribed genes (15). Regardless of hybrid-
ization intensity, all RNA samples yielded AF cDNAs upon RT-
the translated sequences of pancreatic and liver AFP cDNAs from
each species show high amino acid identities inclusive of the
conserved signal peptide (Fig. 7 A–E, which is published as sup-
Fig. 3.
hysteresis (TH, or AF activity) of intestinal, stomach, and pancreatic fluids of
Antarctic notothenioids and Antarctic eelpout. Figure is graphical represen-
tation of data in Table 2. Values of ambient McMurdo Sound seawater are
given at the top for reference. TH represented by gray box, and indicated
value is the difference between MP and FP in degrees Celsius.
Osmolarity, melting point (MP), freezing point (FP), and thermal
GI fluids. (A) Fluorescently labeled AFGPs purified from pancreatic fluid (PF),
serum of the same D. mawsoni specimen. (B) Coomassie blue-stained type III
and a small amount of the ?14-kDa isoform. Purified serum AFP isoform
counterparts (7-kDa RD2 and 14-kDa RD3) (24) were coelectrophoresed as
reference. Insulin (5.7 kDa) was used as a low molecular weight standard. The
?5.7-kDa peptide band in IF may be partly digested fragment of the 7-kDa or
14-kDa AFP isoform.
Cheng et al.
July 5, 2006 ?
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Page 4
porting information on the PNAS web site), indicating pancreatic
AFPs are secreted molecules. Likewise, a signal peptide sequence
precedes the AFGP encoded in the pancreatic AFGP cDNA from
the saffron cod (Fig. 7F); thus, gadid pancreatic AFGPs are also
secreted proteins.
In addition to verifying pancreatic expression at the transcrip-
fluids of the type III AFP-bearing Antarctic eelpout Lycodichthys
dearborni and determined the presence of stomach and intestinal
AFP on the basis of significant thermal hysteresis in the respective
fluid (Fig. 3). Furthermore, we isolated apparently intact 7-kDa
type III AFP from the IF similar to the serum isoform RD2 (24)
(Fig. 4B), supporting the entry of the secreted stomach and
pancreatic AFPs into the intestinal lumen. A ?5.7-kDa peptide is
also present in the IF, which may represent a partially digested
fragment of the 14-kDa (RD3) (24) or 7-kDa isoform.
of plasma proteins (8, 9), and that hepatic synthesis and secretion
is responsible for the high circulatory levels of AF protein in
AF-bearing polar and subpolar fishes (10–15), is based on docu-
mented evidence. It is therefore natural to assume that the same
applies to the bloodborne AFGPs of the Antarctic notothenioid
hepatic AFGP synthesis does not occur in the Antarctic notothe-
nioid fishes, the predominant teleost group endemic to the South-
ern Ocean (1). The absence of liver AFGP synthesis in the day-old
hatchling and 4-month-old G. acuticeps larva (Fig. 2) indicates that
no hepatic contribution to blood AFGPs occurs during the early
posthatch life stages, although blood AF in these AF-deficient
larvae steadily accumulates to adult levels during this time (from
this period of accrual to adult plasma AF concentrations, together
with the absence of hepatic AFGP expression in adults (Fig. 1A),
confirms that liver plays no role in AFGP biosynthesis throughout
the life of notothenioid fishes. Our Northern blot results indicating
the absence of hepatic AFGP synthesis in adults is corroborated by
expressed sequence tags (EST) sequencing projects of two adult
Antarctic notothenioid species included in our analysis (Fig. 1A),
D. mawsoni (L. Chen and C.-H.C.C., unpublished results) and
Harpagifer antarcticus (Melody Clark, British Antarctic Survey,
personal communication), which revealed no AFGP cDNA from
the liver. For D. mawsoni, none of the ?4,700 unique cDNAs
obtained from ?12,000 sequenced clones code for AFGP (L. Chen
and C.-H.C.C., unpublished results). Together with this study, we
have established a body of compelling evidence demonstrating the
AFGP-synthesis null condition of the Antarctic notothenioid liver.
Without hepatic AFGP synthesis, we come full circle to the
fundamental question of the source of the abundant AFGPs in the
blood, decades after the protein was discovered in the Antarctic
notothenioids (5). From the evidence in this study, we propose that
reabsorption of pancreatic- and stomach-derived intestinal AFGPs
is the source of circulatory AFGPs in Antarctic notothenioids,
through the following deduction. We have shown the exocrine
pancreas to be the major AFGP expression site from hatching to
adulthood by Northern blot and immunodetection (Figs. 1 and 2),
and Northern blot analysis reveals the anterior stomach as the only
additional site (Fig. 1). No other tissues we examined by Northern
blot analysis showed AFGP mRNA expression, including hemo-
poietic tissues (head kidney and spleen) and circulating blood cells
(data not shown) that could potentially contribute to bloodborne
PF, and IF (Fig. 3), and the isolation of similar complements of
AFGP isoforms from the PF and IF (Fig. 4A); both pancreatic and
gastric AFGPs expectedly reach the intestine through obvious
pancreas and anterior stomach only, and not from the components
of the lower GI tract, because pyloric ceca and intestine cleaned of
AFGP mRNA expression (Fig. 1 B and C). We observed that the
intestinal AFGPs remain largely undegraded as they transit the
acid and alkali by virtue of high sugar content (?60% by mass) and
the lack of cleavage sites for known digestive proteases in their
repetitive peptide backbone (Thr-Ala?Pro-Ala-)n (4, 22). The
nearly identical intestinal and serum AFGP profiles suggest that
serum AFGPs could be derived from intestinal AFGPs. Reuptake
mechanisms for intestinal AFGPs may exist because rectal uptake
of the protein macromolecule ferritin introduced into the GI tract
has been demonstrated for the Antarctic nototheniid Notothenia
neglecta (26), and a body of experimental evidence exists showing
intestinal absorption of macromolecular proteins for intracellular
Fig. 5.
Type II AFP, herring and smelt. (C) Type III AFP, zoarcoid fishes. Ma and Am are late spring specimens that have greatly reduced AFP synthesis. (D) Type IV AFP,
longhorn sculpin. (E) AFGP, northern hemisphere gadids. (Lower) Same blots hybridized with pancreatic trypsinogen cDNA to verify presence of exocrine
pancreas in pancreatic isolates. Faint trypsinogen hybridization in liver RNA (Pbr, Mo) is likely a result of pancreatic infiltration in liver (32). (Detailed species
information is in Table 1.)
Northern blot analysis of AF mRNA expression in pancreas and liver of nonnotothenioid AF-bearing fishes. (Upper) (A) Type I AFP, winter flounder. (B)
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processing in teleost fishes (27). Collectively, the above reasoning
leads us to propose that pancreatic- and gastric-derived intestinal
AFGPs may be returned to the blood by intestinal and?or rectal
absorption. The secretion of pancreatic and gastric AFGPs, their
transit into the intestine, and subsequent intestinal?rectal absorp-
that would take considerable time, and some excretory loss of
intestinal AFGPs likely occurs. These factors conceivably contrib-
posthatching) observed in the nascent AF-deficient G. acuticeps
larvae (25). Once in the blood, the aglomerular kidneys, a derived
production (28, 29), serve to conserve AFGPs in the circulation.
Renal AFGP conservation and high chemical stability of the
AFGPs imply that, once sufficient plasma levels are achieved, only
maintenance levels of input of GI AFGPs to blood would be
necessary, which could be readily provided by the GI-to-blood
transport even if the process lacks efficiency. Inefficient GI-to-
blood transport would mean significant loss of intestinal AFGPs,
which seems energetically wasteful. Some fraction of intestinal
AFGPs may indeed be ''recycled'' via a blood-to-bile-to-intestine
pathway, because AF activity is found in the gall bladder bile of
notothenioids (results not shown; ref. 35). Given that the liver does
not synthesize AFGPs, bile AFGPs are likely derived from plasma
AFGP via paracellular and?or transhepatocyte blood-to-bile trans-
port in the liver, known to occur for plasma proteins in mammalian
appears to be a constitutive condition in notothenioids (regardless
of age and time of pancreatic tissue sampling), suggesting that
continuous resynthesis to replenish intestinal loss may be an oblig-
atory energetic cost for ensuring intestinal freezing avoidance.
Our discovery of the AFGP-synthesis active exocrine pancreas
fishes (6, 7, 16) is erroneous, and that the reported observations of
hepatic AFGP expression might be of pancreatic origin. The
exocrine pancreas in almost all adult teleost fishes, including
associated with surfaces of the abdominal organs, adipose tissue,
mesentery, blood vessels, and ducts, on and in the gall bladder wall,
and, in some fishes, it also infiltrates the liver along hepatic portal
blood vessels forming hepatopancreas islets (19, 20, 32). Thus, the
reported AFGP synthesis in primary cultures of isolated hepato-
cytes by radioactive labeling (7) and detection of liver AFGP
mRNA in Northern blot with an unusually large amount (50 ?g) of
1% mRNA abundance, as opposed to 5–15 ?g total RNA per
sample used in Northern blots in this study) could have resulted
the converging biliary ductules exiting the notothenioid liver (19).
in the blood behind non-AFGP plasma proteins in radioactive
tracer studies (6) could be reconciled by our proposal that the
source of plasma AFGPs is the secretion and reabsorption of
intestinal AFGPs, expectedly a slow process, and therefore not
synchronized with liver synthesis and secretion of other plasma
The historical focus of polar teleost freeze avoidance studies has
been the primary importance of preventing the blood from freez-
ing, ostensibly shaped by the conspicuously high plasma levels of
AFs in the adult fishes. Less obvious and far under-recognized is
that freezing prevention of the hyposmotic fluids of the alimentary
canal is equally crucial to survival. The endowment of AFs in the
stomach and intestinal fluids (particularly ample in the highly
hyposmotic IF) in unrelated Antarctic notothenioid and zoarcid
(eelpout) fishes (Figs. 3 and 4) and the functional convergence of
pancreatic AF expression in all AF-bearing fishes regardless of AF
type (Figs. 1 and 5) attest to the universal need for preventing the
hyposmotic GI fluids from freezing. This need inevitably arises
for osmoregulation (21, 33), which generates an avenue for the
notothenioids confined to chronically ice-laden freezing waters (3,
22) and for north-polar fishes when their habitats reach freezing
temperatures and become icy. Intake of ice-associated food in icy
GI fluids, which must be prevented to ensure organismal survival.
The presence of high levels of AFGPs in the IF of Antarctic
notothenioids has been recognized in earlier studies (34, 35), but
the source was unknown until the present study. For fishes that
possess other types of AF protein, presence of intestinal AF is
demonstrated for the type III AFP-bearing Antarctic eelpout L.
dearborni (Figs. 3 and 4B) in this study, and recently for the
AFGP-bearing Arctic ice cod A. glacialis (36), both of which show
pancreatic mRNA expression of their respective AF (see L. dear-
born and A. glacialis in Fig. 5). It follows that the intestinal AF
originates from pancreatic AF secretions through the direct pan-
creatic duct connection between the exocrine pancreas and intes-
tine in these species, as well as in fishes bearing type I, II, and IV
AFP (Fig. 5), which show pancreatic expression of AF mRNA
carrying a secretory signal sequence (Fig. 7). Furthermore, besides
Antarctic notothenioids, we found type III AFP mRNA expression
in the anterior stomach of L. dearborni (data not shown) and AF
activity in the stomach fluid (Fig. 3), and stomach expression of the
mRNA of the secreted type of AFP I by winter flounder has also
been observed (12), suggesting that this additional GI source of AF
may also be common among AF-bearing fishes.
The evolutionary confinement of Antarctic notothenioids to
persistently icy conditions in the Southern Ocean, where ice inges-
tion is recurrent and arresting ice growth in their hyposmotic GI
fluids a constant necessity (22), combined with the absence of liver
AFGP expression, suggests that natural selection acted first and
foremost on preventing GI freezing in these fishes. Consistent with
the selection for GI freeze avoidance is the evolution of the AFGP
gene from a pancreatic trypsinogen-like protease (TLP) gene (17,
18) and the exclusive role of GI components in AFGP expression
(this study). Experimentally, wild-caught notothenioid fishes ren-
dered free of associated ice by warming at 1°C acquire ice in their
IF much faster than in their blood after being returned to the
ambient icy waters of McMurdo Sound, Antarctica (22), under-
fluids over other body fluids. The eventual acquisition of ice in the
blood means continual protection of the hyposmotic vascular fluid
pancreas and anterior stomach and has not spread to the liver over
evolutionary time for more expeditious delivery of AFGPs into the
blood seems to defy reasonings of evolutionary parsimony. A
possibility resides in the expression of the evolutionarily related
AFGP and TLP genes being potentially still under common tran-
scriptional control, as indicated by the chimeric AFGP?TLP genes
that are transcriptionally active in the pancreas (18). Without
separate transcriptional control, concomitant expression of high
levels of a serine protease by the liver could lead to unfavorable
physiological consequences. Another aspect of Antarctic notothe-
nioid physiology regarding freeze avoidance, alluded to earlier, is
the AF-deficient (up to 3 months posthatching) but paradoxically
freeze-resistant condition of young larvae that apparently rely on
intact skin and under-developed gill structures as effective physical
barriers to ice entry during that time, instead of fully AFGP-
fortified blood (25). Collectively, these physiologies highlight a
general need for a greater awareness of evolutionary creativity in
organismal adaptations.
This study establishes active pancreatic AFGP synthesis and the
AFGP-expression null state of the liver in Antarctic notothenioid
fishes, bringing a significant perspective to teleost freeze-avoidance
physiology. It also reveals that the long-held paradigm of hepatic-
Cheng et al.
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vol. 103 ?
no. 27 ?


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The oil spill in the Gulf of Mexico is threatening to derail the Senate climate bill, which has a provision for expanded offshore drilling. "When you're trying to resurrect a climate bill that's face down in the mud and you want to bring it back to life and get it breathing again, I don't think you can have offshore drilling against the backdrop of what's transpiring in the Louisiana wetlands," said Richard Charter, an adviser to the group Defenders of Wildlife.
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TripAdvisor Reviews Georgian Lodge Hotel Bradford-on-Avon
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Royal Crescent, Gardens, American Mus., Stonehenge
... skin in their place.
Next we took the car and drove to the 'American Museum in Britain' on the outskirts of Bath. This museum was founded by three businessmen doing business in both Britain and America who realized that the only American museums were in America. They felt it would improve relations between the countries if the British had a place to learn about American history. Using things ...
Tour of Bath
... tour didn't finish until after 1 pm. We grabbed a quick bite to eat at Subway and used the bathrooms. You had to buy something to get the code that would unlock the restroom!
After lunch we went to the 500 year old Abbey. It was bombed by Germany in World War II in retaliation for the Allied bombing of history sites in Germany. The Abbey was all but destroyed, however it was restored in the 1950s and 60s and it ...
Day 5 Swindon to Weston super Mare ~ 78m
Wow! Today was the best day so far, purely because of the Cheddar Gorge. It was amazing breathtakingly beautiful to descends between it's cliffs. I'm so glad I went there and would love to revisit. Cycling there made it such a rewarding experience. The rest of the day was pleasant too, punctuated by ...
Last day in Wiltshire
... an Elderflower cordial.
Only a short distance away was Castle Combe, another unspoilt village tucked away in a pretty valley, away from the main roads. It was so beautiful, so tranquil and again we wandered around in the light rain. The little houses are so cute and well looked after, and a clear little brook tinkled under the stone bridge. Being in the Cotswolds, most of the buildings are made of the local limestone, which weathers to a mellow golden ...
Today we went to the city of Bath. We saw the Romans Baths where the Romans used to take baths and go to socialize. the water came from an under ground water spring and was hot. The Romans believed that the waters had healing powers and the goddess Sulis Minerva was who they worshiped there. I liked how we saw what they wore back then! I liked their pictures in the gem stones that were once on their rings. The gem stones fell out of the rings when the people were in the water. ...
Other places to stay in Bradford-on-Avon
BEST WESTERN Leigh Park HotelLeigh Road West, Bradford-on-Avon | 3 star hotelfrom $139
Woolley GrangeWoolley Green, Bradford-on-Avon | 3 star hotelfrom $199
Bradford Old Windmill4 Masons Lane, Bradford-on-Avon | Hotelfrom $138
Widbrook GrangeTrowbridge Rd, Bradford-on-Avon | Hotelfrom $160
Swan Hotel1 Church St, Bradford-on-Avon | Hotelfrom $147


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Registration takes less than 1 minute.
Meanwhile, lane outages in the existing locks for maintenance could reduce available capacity for vessel traffic by up to 50 percent through the end of the month, according to maritime support services provider Gulf Agency Co.
The U.S. Government Accountability Office said in a report issued Friday CPFA requirements have increased the overall cost of shipping food aid by an average of 23 percent from April 2011 through fiscal year 2014.
Survey of 60 shippers, carriers, LSPs and brokers rated Savannah and Charleston as the top performing ports in the United States, while Oakland was received the worst scores in chassis availability and overall port performance.
Exports helped drive record throughput in September at the Port of Long Beach, but were the main reason the Port of Oakland experienced a 4.8 percent drop in container volumes.
The derailment of a CSX train carrying Bakken crude, which resulted in a fire that forced the evacuation of hundreds of area residents, was caused by a defect that was missed on two separate inspections, according to the Federal Railroad Administration.
In a response to the ITF recommendations, the International Chamber of Shipping said it prefers a fuel levy, rather than an emissions trading scheme, to reduce greenhouse gas emissions from shipping.


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Release Date:
October 26, 2010
Fitness expert and personal trainer Bernadette Giorgi leads this full body exercise program, designed to offer both a cardiovascular workout, and intense muscular sculpting and toning, all through the use of Pilates based techniques, and the integrated use of a power ball. Cammila Collar, Rovi
- Genre: Health & Fitness
- Category Toning & Strength Training
- Run Time: 52 min
- Language: English
other Info.
- Online Item #: 12984717
- Store Item Number (DPCI): 246-06-0385
- Made in the USA or Imported


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Wysp: realize your creative potential
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Learn How Human Business Works - Beyond Social Media
As they say, what happens in Vegas stays in Vegas - but what if you could share your secrets with full discretion and glimpse into the hidden lives of the people around you? Confessions is a public art project that invites people to anonymously share...
We help you discover events, places and activities worth doing. Find amazing parties, hidden bars and other things to do in your city.
I usually tell people that everything I learned about being an entrepreneur I learned by F'ing up at my first company. I think the sign of a good
When you see your ex at a party: When your boss catches you not doing your work: When you are crossing the street and someone honks at you to hurry up: When you were a kid and got to go to the playground: When you find a friend you can act ...
An interesting interpretation of the printed word.
Practical Tips for Productive Living
"THIS DAY" is a web content page that shows what happened on that day in the past, at some place in the aworld.
Create your own generative art with Silk.
amazing talent rate, comment, subscribe.
This is probably the most beautiful dance show ever, the dancing was perfect. The freezes and movements were so balanced. Please do not copy or distribute th...
REPURPOSING is so hot right now. At least if you're on or , that is. If the most hardcore "upcyclers" are to be believed, there's no such thing as trash anymore. These folks turn old yoga mats, washing machines, and even dumpsters into cool items wi...
Welcome to Planet eBook, the home of free classic literature. We offer an assortment of classic novels and books in electronic form which you are free give to your friends, classmates, students, anyone!
Fifty useful life tips & tricks that you can implement this instant. Increase your efficiency and ability to succeed by modifying or adding these little habits into your daily existence.
Steampunk clothing and watches. We specialize in pocket watches and other steampunk clothing items with exposed gears!
Write by hand on your computer! Go to www.pilothandwriting.com, turn your handwriting into a digital font and send handwritten e-mails to your friends.
The the essence of any good bucket list consists of overcoming fears, achieving goals, realizing dreams and even simple pleasures. Whether it's an exotic adventure half-way around the world or something simpler, like spending more time with your fami...
RelayRides blogger Brad Iger runs through some of the best driving tips for the City of Angels.
Wow! That's easier than I thought it would be! Thanks!
& .
Last week, someone asked me to make a legs tutorial. I promised with her and now it is . I also include hand & foot tutorials in it ^^ It's may not ...
Artist Rashad Alakbarov from Azerbaijan uses suspended translucent objects and other found materials to create light and shadow paintings on walls. The best part is that you can easily create somet...
http://www.pianojuggler.com Dan Menendez juggler extraordinaire
If you don't know how to code, then you can learn even if you think you can't. Thousands of people have learned programming from these fine books:
A collection of fill-in-the-blank forms for everyday correspondence.
Self-Improvement Resource
Credit goes to the people who created these sets , sadly that was not me .. Really good interpretations (:
Born to be alive!
For all fans of graphic design we are happy to present the list of the most well-made and absolutely fresh font types - available for a free download!
Why Men Should Marry Rythmic Gymnasts
If you've ever been convinced by a salesperson that you truly wanted a product, done something too instinctively, or made choices that seemed entirely out of character, then you've had an idea planted in your mind.
'The Avengers' Reimagined As Medieval Knights - DesignTAXI.com
Quotes about mitch hedberg. Explore and add your favorite quotes to an ever-growing collection of popular quotations about mitch hedberg.
I love the idea of watching movies with just your Internet connection and a computer and no strings attached. Although some of you may think that this is not legal, i would say "why not try something ill-legal today". There are lots of websites which...
a digital relaxation experience
This is a planned maintenance period.Please check back after 7:00 PM Pacific Time.
Funny t-shirts on high quality soft t-shirt, cool shirts and retro vintage tees
I posted a picture about 1 week ago on Instagram, Facebook and Twitter of me cutting glass using just string. I got LOT'S of requests for a tutorial on how I DID THIS! So here it is. FINALLY! You will
Cutting pellet by Isao Machii (Guinness World Record holder). His Youtube Official Page http://www.youtube.com/user/syuushinryuu samurai katana tachi isao ma...
(1960-) is one of the best fiction writers in the world in my opinion. His work covers novels, short-stories, children's books, comics, film, television - pretty much the whole pop-culture gamut.
The name of the mix pretty much says it all ... try it
The official blog of America's favorite frozen dough
Some of these will enlighten you, others will frighten you, and this one right here will ruin your childhood... via via via via via via via via via via via via via via via via via via via via ...
[See in a PDF file.]
This male bluebird was protecting his nesting box from swallows and was photographed in Ottawa ON Canada with a Nikon D3s camera and Nikon 600 mm lens
go on, click Like, it makes me feel better :)
This wonderful work has done by a very talented Belgian painter, illustrator, portraitist, caricaturist and photographer Ben Heine. This creative artist was born in Abidjan, Ivory Coast. He Studie
they are all over pinterest.... (i still am not signed up for pinterest because i don't have time for one more thing but browsing is fun!) so we made one. i took a box of 64 crayons and took out the blacks and browns. i used another small box and dou...
Created in twenty-four boozy hours by , , and at . Powered by , , and what's left of our livers.
3D animated origami instructions to make a flapping bird, peace crane and a football shirt in 15 languages.
04.11.11 Troy Turner
Stereomood is the emotional internet radio that provides me music that best suits my mood and my activities and personalized playlists for specific emotions and events.
Direction, Cinematography, Editing - Dave Kaufman just a little mix and match.
Thousands of free ebooks, pre-formatted for reading on your computer, smartphone, iPod, or e-reading device - ePUB, Kindle, eReader, PDF, Plucker, iSilo, Doc, RTF, Mobipocket, Newton Paperback, and zTXT ebooks ready to go!
Find the best book deals and sales from the most popular book stores. Enjoy discounts on everything from e-books to textbooks with book coupons from RetailMeNot.com.
Pandora radio is the personalized internet radio service that helps you find new music based on your old and current favorites. Create custom web radio stations, listen free.
Scribbler Art Toy :: Zefrank.com
Folding Trees - the best tutorials and paper creations online
ago the cost was seven years to a lifetime of servitude for average people making a one-way crossing of the Atlantic. Now it can be funded by a few days of waiting tables.
When we posted this story about Phil Hansen and his incredible works of art our viewers were blown away; some even compared him to da Vinci.
sevenwondersoftheworld and the seven world wonders


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Proof Pics: Allen Iverson Got Caught Sleeping
Former NBA star Allen Iverson forgot the cardinal rule when it comes to groupies: never sleep.
Morning Wood: Method Man
Another week, and yet another set of bathroom mirror peen pics have leaked all over the Internet. This time, the "victim" is Wu Tang Clan rapper Method Man, who has to explain to his mother that he knows she didn't raise him to be dumb like other rappers, but somebody hacked his cell phone, blah blah blah.
Morning Wood: Lil Romeo does not live up to his name
Once again, a celebrity forgot to lock his cell phone with a simple password that would save his dear mother from embarrassment when photos of a sensitive nature leak online.
Rapper-turned-actor Lil Romeo is desperately attempting to scrub the Internet clean of these smutty photos that leaked online yesterday.
Morning Wood: Snoop Dogg's son lets it all hang out
I'm not sure how old Snoop Dogg's son is, but I'm sure his age won't matter to you thirsty Cougars. The young man leaked a photo of his family jewels onto the Internet earlier today. Let's just say he is very mature for his age. I'm impressed and I don't even eat meat.
Morning Wood: Joe Budden's lil' buddy ***NSFW***
A graphic photo exposing rapper Joe Budden's little buddy is on the Internet. That must mean a new season of a reality show is about to get underway?
For those of you who don't know, Budden (along with "video model" girlfriend Tahiry Jose) is a new cast member on season 3 of Love and Hip Hop New York.
The reality TV series, produced by Mona Scott-Young, features returning cast members Erica Mena, pseudo-singer Olivia and her mentor/manager, Rich Dollaz. Cast member Yandy Smith introduces her baby daddy Mendeecees Harris to the show's viewers.
Newcomers to season 3 include media personality Raqi Thunda, former G.O.O.D. Music rapper Consequence and his baby mama Jen "The Pen" Bayer.
Season 3 of LHHNY premieres on January 7, 2013.
A loyal reader writes:
while he was out in cali we got in that nigga's phone. now we see why all his girls end up cheating on him.
Sandrarose.com is a family-oriented blog. However, I am publishing this pic because it falls under the category of humorous news. You must be 18 and over to view the pic.
There is no need for you to email me complaining about NSFW photos. You have to make an extra effort to scroll all the way down just to see these pics. So don't blame me for your curiosity. Please consider your co-workers and your job status before clicking the link!
The following image is NOT SAFE FOR WORK! Proceed at your own risk!
Morning Wood: Soulja Boy ***NSFW***
Once again, has-been rapper Soulja Boy is feeling neglected by his former fans. So what does he do? He makes a contribution to pr0n by tweeting a partially nude photo of himself. Judging from the photo of his diseased rhinocerous pizzle circulating the Internet, he should hurry to the nearest STD clinic.
Not sexy at all…
Morning Wood: Stevie J ***NSFW***
A loyal reader sent in a link to a graphic, entirely NOT SAFE FOR WORK photo of Love & Hip Hop Atlanta cast member Stevie J gripping his impressive wood.
My guess is that this peen pic was released in response to all the rampant rumors about Stevie J preferring to have his prostate tickled by men who dress like women.
There is no need for you to complain in my comments section about me posting NSFW photos. You have to make an extra effort to scroll all the way down just to see these pics. So don't blame me for your curiosity. Please consider your co-workers and your job status before clicking the link!


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SALT LAKE CITY, Oct. 10 /PRNewswire/ -- Amedica Corporation, an orthopedic implants company focused on silicon nitride ceramic technologies, announced today that a paper it presented on the in-vitro testing and evaluation of its silicon nitride ceramic bearings for total hip arthroplasty won the HAP Paul Award from the International Society for Technology in Arthroplasty (ISTA). The prestigious HAP Paul Award, which was presented to Amedica at ISTA's 20th Annual Congress, held October 4 - 6, 2007, in Paris, France, is given for outstanding research in new technology for joint replacements.
The award-winning research paper, "In Vitro Performance of Silicon Nitride Ceramic in Total Hip Bearings," will be published by ISTA as a special article in the Journal of Arthroplasty. The paper demonstrates that Amedica's MC2(TM) silicon nitride based ceramic bearings, being developed for reconstructive hip, knee and spine implants, have the combination of strength, toughness and wear resistance characteristics that may overcome the limitations of currently available bearings.
The paper was authored by a team led by Ashok Khandkar, Ph.D., and included B. Sonny Bal, MD, University of Missouri, Columbia; Dr. Aaron A. Hofmann MD; University of Utah; Dr. Ian C. Clarke, Ph.D., Loma Linda University; Dr. Alan Lakshminarayanan, Ph.D., Amedica; and Mohamed N. Rahaman, Ph.D., University of Missouri-Rolla.
The team of collaborators compared the fracture strength and in-vitro wear characteristics using hip simulators of Amedica's Micro-composite Ceramic, or MC2, bearings with current state-of-the-art bearings. The data presented at ISTA's Annual Congress demonstrated that the silicon nitride femoral heads had substantially greater burst strength than similar 28 mm femoral heads made using the strongest commercially available ceramic.
"We are delighted and honored to win the HAP Paul Award for our work,"
said Ashok Khandkar, Ph.D., CEO of Amedica Corporation.
|SOURCE Amedica Corporation
Copyright©2007 PR Newswire.
All rights reserved


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Frat Jock Shows Off Naked On His WebcamThursday, November 28th, 2013
Jaxon has a pretty consistent routine of going to gym to exercise, hit the showers and head back to his dorm room. It's customary for athletic college jock to make use of his camera phone to measure the results of his routine work out results and sometimes admire his body completely naked. Today Jaxon makes his webcam scene public and we all get to drool over this muscle jocks flawless beauty!


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It's not just the new iPod touch that's gotten the FCC treatment today -- what we can only assume is the new Apple TV has also just turned up in some newly published documents from the agency. Unfortunately, there's no pictures this time around (internal or otherwise), but there are a number of test reports that confirm that the device does indeed pack Bluetooth and dual-band WiFi (802.11 b/g/n at 2.4GHz and 802.11 a/n at 5GHz). And in case you're wondering where the label goes -- it goes there.
Buy from $65
- Key specs
- Reviews • 112
- Prices
- Discussions
- Type Audio / video player
- Video services iTunes, Netflix, YouTube, Other
- Audio services iTunes
- Video codec support h.264 / AVC, Motion JPEG, MPEG-4, Quicktime
- Audio codec support AAC, MP3, WAV
- Video outputs HDMI (1 outputs)
- Audio outputs via HDMI, TOSLINK (optical)
- Released 2012-03-16


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Westech Industrial Ltd
Westech Industrial Ltd is a leading supplier of instrumentation and process control equipment to Canadian industries and abroad. Since our inception in 1966, we have developed a reputation as a source for quality solutions.
Moore Industries-International, Inc.
See our NEW dual input temperature transmitter, plus IEC 61508 Functional Safety instrumentation, signal conditioners, distributed I/O, fieldbus products & more that help you effectively interface field processes with computer-based systems and readouts.
14 Companies with Results for "Height Gauges"
|1 - 10 of 14
B&B Electronics
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B&B Electronics has been designing and manufacturing industrial-grade data communications equipment for 31 years, working with everything from legacy serial devices to the latest in industrial Wi-Fi. More
Heidenhain Corp.
333 E. State Pkwy. Schaumburg, IL 60173 United States of America
Come see us at IMTS - Booth E5248 - Sept. 8-13, 2014 FOLLOW LINK: http://www.imts.com/visitor/exdir/exhibitor_details.cfm?exhid=00000664 ...
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|1 - 10 of 14


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Don James McLaughlin is the recipient of the Marguerite Bartlett Hamer Dissertation Fellowship, awarded by the University of Pennsylvania's McNeil Center for Early American Studies. The award is a yearlong fellowship for the 2015/16 academic year.
Alumni News
Sunday, May 10, 2015
Wednesday, April 29, 2015
Ted Howell Quoted in New York Times
Ted Howell, a recent alumnus of the program and a PhD student at Temple University, is getting attention for breaking ground with an innovative new course at Temple. "Cli-fi: Science Fiction, Climate Change, and Apocalypse" focuses on the new genre of "Climate Fiction," which shows the possible apolcalyptic outcomes that are inevitable if climate change continues as predicted. This course is getting media attention for the way it brings together literature and cutting-edge scientific findings. The course focuses on this new movement in literature (exemplified in such works as The Wind-Up Girl), which harnesses the concerns of a culture that feels the threat of apocalyptic climate change. In addition to getting students thinking about the intersection between climate science and fiction, he also encourages his students to participate in the wider climate change conversation by posting all their work to a blog. He was recently featured in a Reuters article, a New York Times feature, as well as of course a write-up in Temple's English Department Blog.
Posted by Katharine McIntyre at 3:49 PM No comments:
Wednesday, February 18, 2015
Rebecca Hepp ('13) Transitions to Senior Associate Editor Position
Rebecca Hepp, a 2013 graduate of the Graduate English Program, is now a senior associate/web editor for Jobson Healthcare Information's Review of Optometry magazine, a national, monthly magazine based out of Newtown Square. "So far I am really happy with the move!" Rebecca says. "It's mostly print now, but they brought me on board to help revamp the website and give it some much needed TLC."
Posted by Katharine McIntyre at 11:18 PM No comments:
Laura Heinrich Now Teaching at Arizona Charter School
Villanova Grad English alum Laura Heinrich ('10) just got a new job. According to a recent update, she is now working at a charter school in Phoenix, Arizona, with the "Great Hearts" charter school system. The rest of her update is below:
Check out our "Jobs" page for more information about working at a Great Hearts school.
I am finally doing work I love, in Phoenix (where it is gorgeous right now and will be HOT in a matter of weeks). I'm teaching poetry, history and literature for middle and high school at a charter [school called "Great Hearts."] It is a very Liberal Arts heavy education, and I think a lot of people who are struggling to find a niche in the academic world might end up as happy as I am in this one.
Check out our "Jobs" page for more information about working at a Great Hearts school.
Posted by Katharine McIntyre at 11:01 PM No comments:
Friday, November 21, 2014
Ted Howell
Ted Howell (English MA '10, now a PhD candidate at Temple University) presented a well-attended paper on "Howards End and the Anthropocene" at the Modernist Studies Association annual Conference in Pittsburgh, PA.
Posted by Katharine McIntyre at 1:11 PM No comments:
Tuesday, October 7, 2014
Don James McLaughlin
On Tuesday, September 20 The University of Pennsylvania English Department selected Don James McLaughlin (Villanova Grad English Class of 2009) as the winner of its annual William Patrick Day essay contest. This was a contest in which any grad student can submit a piece of writing completed in the last year. Two professors then judge the submissions anonymously. McLaughlin's piece is part of his dissertation--tentatively titled "Color-Phobia: Rabies, Race Prejudice, and the Mad Cry of Analogy in U.S. Antislavery Print Culture."
Posted by Katharine McIntyre at 1:47 PM No comments:
Wednesday, September 10, 2014
Lydia Browning Welcomes New Baby
Posted by Katharine McIntyre at 12:05 PM No comments:
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Thank you so very much for a wonderful weekend.You were very acomidating and friendly .the rooms were cozy and extremely clean.Looking forward to coming back soon.we went to Rockport for the day and it was beautiful.
Thank you so very much for a wonderful weekend.You were very acomidating and friendly .the rooms were cozy and extremely clean.Looking forward to coming back soon.we went to Rockport for the day and it was beautiful.
Lakeside Motel Cabins Marina is located on the north end of beautiful Cobbosseecontee Lake near Augusta, ME. The cabins and motel rooms are quaint and clean. First impression is a 1950's Motel but motel rooms have flat screen TVs, WiFi, Microwave, and refrigerator....everything the Big Name Hotels have without the beautiful view and setting. My favorite time of the day... More
We booked Lakeside as our base camp for 7 days of bass fishing this past July. Landed in one of the cabins - it was smaller than I had expected but it made up for that in comfort. The beds were comfortable, the WiFi was good, the kitchen worked well for us, and the bathroom was spacious. Clean and comfortable.... More
I rented a room at this motel because of its proximity to the Maine State YMCA camp where I was having a 100th anniversary reunion. I have to admit that my expectations were : clean room on the lake front. I definitely got that. Our room (2 double beds/ or maybe queens?) was very comfortable. It was very clean and... More
My family and many other members stayed here for the third week of July. Andy and Sheree were tremendously accommodating. We especially enjoyed meeting at the picnic tables for early morning family coffee and donuts. We also had to prepare a family meal so having a full kitchen in the
I have stayed at Lakeside with my family since my boys were toddlers. Now they are in high school. Each year we go back to this glorious place on the shores of Lake Cobbosseecontee! The owners go above and beyond each day to ensure you are taken care of, A true family run business with the customer as the top... More
I've stayed here more than once recently when I was traveling alone with my little dog, Rusty. I have only stayed in one of the little single cabins along the side of the main building so I can only comment on that. In general terms, it was very basic but totally sufficient to meet my need. I really don't have... More
We had a family emergency and needed 8 rooms in a pinch. Not only was the staff at the Lakeside accommodating they were professional and sincere. The facility met all our needs and even offered an outdoor location to sit, relax and visit after a very long day of travel and family business. The cool evenings and beautiful fall colors... More
I really wanted to love this little place. It seems to have lots of great reviews from loyal followers. I love Mom & Pop businesses. But in the end, I would not stay here again. Basic info: stayed in hotel room, second floor, no kitchen. Family gathering Pros: Easy to get to. Beautiful views. Amazing sunsets. Grounds are well kept.... More
The NATURAL beauty of this Maine lake, the lovely voices in the Loon Choir every evening, the glorious green of the trees and fields, and even more, the kind, generous and accommodating NATURE of owner's Andy and Sheree and staff take guests back to the days of true "neighborly -- ness". We very recently spent 15 days (in 4 different... More
Senator Inn & Spa
#4 of 8 in Augusta559 reviews
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BEST WESTERN PLUS Augusta Civic ...
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Select Local Merchants
Tilton FitnessRead MoreTurn over a new leaf working out at Tilton Fitness in Galloway. With food just the way you like it, this gym elevates your restaurant experience just on the level of taste. At this gym, everyone will find something they love — kids included! It's easy for your workout to become complacent, that's why you should consider circuit training! A great body starts with a high-intensity workout. That's why everyone recommends cross fit to transform your fitness. When you're looking for a round of cardio that's fun too, then you'll want to try the terrific aerobics offered here. Sign up for one of these spinning classes and spin your pedals until you can't cycle anymore. These Zumba classes provide a full-body workout that feels more like a dance party than a workout. Keeping your form is incredibly difficult, but when you sign up for a personal trainer, they'll ensure your form is looking great for maximum impact. At Tilton Fitness, you can learn quick and effective kickboxing techniques that will tone and enhance your body. Parking is plentiful, so guests can feel free to bring their vehicles.323 S Pitney RdGalloway, NJ US
Tilton FitnessRead MoreMotivation is the name of the game at Egg Harbor Township's Tilton Fitness. Next time you're in the neighborhood, this gym should be at the top of your list with their amazing restaurant. This gym welcomes kids, too, so you can feel good about bringing the whole family. Move your whole body with their intense aerobics, forcing you to take your body to a whole new level. Take advantage of the personal trainers here to help you keep your form and get the results you intend. Dance out your stress and worries during a fun and entertaining Zumba class. Get your heart pumping and your body moving with a group spinning class. Take your workout goals seriously with the high-intensity and fast-paced results of a cross fit workout. You'll definitely feel circuit training the next day, reminding you you're one step closer to your goals. These kickboxing classes will teach you how to properly use your upper and lower body during self-defense. Parking is plentiful, so patrons can feel free to bring their vehicles.3022 Hingston AvenueEgg Harbor Township, NJ US
Tilton FitnessRead MoreThrow on some shorts and sweat it out at Tilton Fitness in Hamilton Township. Circuit training will show you results fast. Book your next kickboxing class at this establishment and kick and punch your way to your fitness goals. With one of these Zumba classes, you can dance your way to your fitness goals and get your sweat on. Personal trainers are also available to work with those who prefer an individualized work out regimen. Have fun at aerobics classes with the satisfaction of knowing you are burning calories. Take your workout goals seriously with the high-intensity and fast-paced results of a cross fit workout. If you love indoor cycling, you will love the high-intensity spinning classes that this establishment has to offer. Parking is plentiful, so patrons can feel free to bring their vehicles.5401 Harding HighwayHamilton Township, NJ US
Elite FitnessRead MoreYou're on your way to a better workout when you join Long Beach Township's Elite Fitness. Tone your body and strengthen your core with a group kickboxing class. Parking is plentiful, so visitors can feel free to bring their vehicles.12904 Long Beach BlvdLong Beach Township, NJ US
Cycle On StudioRead MoreTaking a road bike in a spinning studio may seem like a recipe for pedaling into a wall, but at Cycle On Studio, it's a recipe for a more realistic workout. During the studio's Kinetic Rock and Roll training sessions, cyclists work out on their own bikes, which they lock into indoor-appropriate frames that provide lifelike side-to-side rocking to challenge the same muscles engaged on the biking trail. This is just one of the studio's many classes that cast off one-size-fits-all workouts. Even during traditional spinning sessions on NXT stationary bikes, students can opt to wear a MYZONE belt that monitors and tracks all of their exercise activity in order to make the classes more efficient. With or without the belts, instructors infuse these workouts with high-energy climbs, interval training, saddle work, and virtual rickshaw rides.3101 Rt 42Sicklerville, NJ US
Anytime Fitness, Berlin NJRead MoreWhen you need something to do on your day off, visit Anytime Fitness, Berlin NJ in Berlin Township and indulge in a day filled with fun and adventure. Get your heart pumping and your feet moving with a highly energetic Zumba class. The professional spinning instructors aim to make your workout fun, sweaty and exciting. Impress your friends and family with your kickboxing skills. Take advantage of their excellent personal trainers to ensure that your fitness goals are met on the time table you set. A great body starts with an excellent program of cross fit. Parking is plentiful, so visitors can feel free to bring their vehicles.200 Route 73Berlin Township, NJ US


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Separate names with a comma.
Discussion in 'Revo Technik' started by ANDYTQ, Apr 14, 2013.
Hi guys how much for the select and select plus units.thanks Andy
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Hi Andy, neither of those switches exist. They're both old products now. The SPS is the only switch we offer: Serial Port Switch (SPS)
The SPS is now £99+vat


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More English Pointer Puppies and Dogs for sale.
English pointer male ROCKSOLID BIRD DOG KENNEL in Arizona
English Pointer, Male Steve Jarvis Bird dogs in Como, Texas
English Pointer Setter Puppies Steve Jarvis Bird dogs in Como, Texas
English Pointer puppies Fox Run Kennels in Brook Park, Minnesota
Trained English Pointers Outback Kennels in Kensington, Kansas
Trained English Pointers Outback Kennels in Kensington, Kansas
Elhew & Southern Doctor Bloodline Plum Branch Kennels in Linton, Indiana
English Pointer Female Plum Branch Kennels in Linton, Indiana
CH Sired English Pointer Puppies bo... Indian Creek Gun Dogs in Franklin Lakes, New Jersey
***READY TO HUNT-FINISHED ENGLISH P... Wells Fargo Pointers in Wilton, California
SOLD-STARTED ENGLISH POINTER Wells Fargo Pointers in Wilton, California
Well Started English Pointer Female... Warriors Mark Kennels in Ginter (Houtzdale), Pennsylvania
SOLD 2 English Pointer Pups Godfather Gundogs in Elizabeth, Colorado
Started English Pointer Females!!! Little Wing Gun Dogs in Flemington, New Jersey
English Pointer Pups expected Octob... Little Wing Gun Dogs in Flemington, New Jersey
English pointers for sale Backrun Pointers in Manheim, Pennsylvania
Rod Lane Gun Dogs Rod Lane in Battleford Sask., Saskatchewan
Pinehill Doc Holliday X Dirt-Broke Cowgi... DIRT-BROKE KENNELS in KINGSTON, Georgia
Stylish Started Elhew Breed English Pointers - English Pointers in TUCSON, Arizona - Sunrise Kennels
Elhew breed English Pointer puppy training. Top Champion breeding. Puppies showing great style, intensity, and bid ability at such a young age. Ready for sale to a great home like yours. Great for pheasant , quail and all upland. Super family members
Here is a video link: http://youtu.be/mLeM2CjnGBI pup10
Give us a call 520-907-5690
Les Greer Sunrise Kennels.
Ad location: TUCSON, Arizona
Ad created: Sep 16, 2012
Contact Information
Contact Name: J.L. GREER
520-733-1172Contact breeder through email Click here to visit website
Sunrise Kennels Breeder Information


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So found a local posting the other night, emailed in the morning and just met up with the guy.
My size, it will need a bath, will give it new cables/housing, new pads, will swap tires for some I already have, will need to look at the rear hub/bearing as they make some noise. Wheels look OK giving them a quick spin. Bontrager bar, stem, post, WTB tires, Avid brakes, XT cranks.....it looks good to me.
And only $160 bucks.
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Racist or historically accurate?
taylor swift
Not a Swiftie.
Move over Trump there's a new raging asshole POTUS candidate….
'You're not a friend to those with noses'
Who will be next??
What, no cookies?
Swifty gives us the lowdown…
I'm rooting for a Rihanna-assisted punking on live TV.
Pop princesses are breaking off into warring fragments….
China isn't Apple or Spotify, it turns out.
Minaj is pissed…. Swift is making it all about her…. surprised?
Popdust—home of crotch watch.
Simple slouching or cause for concern?
What balls! I mean, you know. Not actual balls.
Haha, that funny Taylor!


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Julius von Grawert
From Wikipedia, the free encyclopedia
Julius August Reinhold von Grawert (1746–1821) was a Prussian general. During the Battle of Jena-Auerstedt on 14 October 1806, he led a division under Frederick Louis, Prince of Hohenlohe-Ingelfingen at Jena.
As Generalleutnant, Grawert commanded the Prussian auxiliary corps attached to French Emperor Napoleon I's Grande Armée during the French invasion of Russia. Grawert was replaced by Ludwig Yorck von Wartenburg in 1812.
- Petre, F. Loraine. Napoleon's Conquest of Prussia 1806. London: Lionel Leventhal Ltd., 1993 (1907). ISBN 1-85367-145-2
|This biographical article related to the German Army is a stub. You can help Wikipedia by expanding it.


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Aggie MacKenzie, of Kim and Aggie fame, used to study round the corner in the old Aberdeen College of Commerce.
What does AC stand for?
AC stands for Aberdeen College
This definition appears very frequently and is found in the following Acronym Finder categories:
- Organizations, NGOs, schools, universities, etc.
See other definitions of AC
Other Resources:
We have 212 other meanings of AC in our Acronym Attic
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- Aberdeen, Scotland, United Kingdom - Dyce (Airport Code)
- Abstract State Machines, B and Z (Conference)
- Al, Bill, and Zorn Manufacturing, Inc.
- Albendazole (drug)
- Algemeen Bestuurlijke Zaken (Dutch: General Administrative Affairs; Netherlands)
- Ambulanza (Italian: Ambulance)
- Anterior Border Zone
- Asian Boyz (street gang in Long Beach, California)
- Aachen (Germany)
- Abdominal Circumference (obstetrics)
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Vicky left university and applied for an HNC in Electrical Engineering at Aberdeen College.
Aberdeen College for School/FE College Community Enterprise
I've enrolled on a communications course at Aberdeen College, and I'm looking forward to getting my first job - at the age of 31
The University of Glamorgan with its slogan "You Live, You Learn" was only outspent by Aberdeen College and the University of Salford according to research carried out by AC Nielsen MMS for Channel Four.
Robert Laird, 35, a personal assistant at Aberdeen College, has been honoured with the profession's highest award.
Hay holds a degree in business studies from Aberdeen College of Commerce.
Michael Ross is a graduate of the Aberdeen College of Commerce, in Scotland, and the Dresser Industries Technology School, in Houston, Texas.


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Two Suns Inn Bed & Breakfast
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
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- Continental Breakfast
- Free High-Speed Internet
- Wheelchair accessibility
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- Pets allowed
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TripAdvisor Reviews Two Suns Inn Bed & Breakfast Beaufort
Travel Blogs from Beaufort
Botany Bay Plantation
... are two agricultural fields planted with corn, sunflowers and wheat, sesame, millet, Egyption wheat all to provide food for the wildlife that live there. Portions of the fields are left fallow to grow native vegetation. This habitat diversity is good for all wildlife.
The last owners (1976 -2007) the Meyers left the property to the State and the Department of Natural Resouces opened the plantation to the general public in 2008.
We had fun at McConkeys Jungle Shack, ...
1st Stop-Johannesburg!
... be refreshed for camp. Please pray for the teaching and testimonies, that God would be honored and there would be many decisions to follow Christ! A good many of the team members have made this trip before; please pray that that those who are going for the first time would stay healthy and not be too homesick! I have loved seeing their excited faces as leaving day has drawn closer!
A park under the forest
December 31,2012
Edisto Island, SC
In Live Oaks Campground you can hear
the surf pounding against the beach but, you'er not at the beach. The
smell here is not the smell of the ocean, it is the smell of the
smell of a salt marsh. Here it smells of the earth's primordial soup,
stirred twice a day by forces even greater than the ocean itself.
Here, is a true Maritime Forest filled
with giant long ...
Look what I saw!
Yesterday on my walk I saw something really exciting! Mommy called it a 'gater. It was on the bank of the pond near the big white birds. Mommy made daddy hold me while she took a picture. She said it was just a baby, but still didn't want any of us to get too close. I think she was scared 'cause the picture is a little blurry. Mommy said they like cute little dogs and I ...
Always better together!
... to be wondering around like us. Most conversations are
superficial, "Better here! At least we are not shoveling snow!".
Answer the question, "Where are you going next?" and information
is readily offered, traded. We are comfortable here, still we live
in the promise of new places. Yes, we know, new will always fade
into same.
Soon we will move on from here. We'll
hang our bird feeder in a ...


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For many of New Zealand's indigenous plants, the Māori name is the 'common' name, and English names are rarely, if ever, used; think rimu, tōtara, kauri, pōhutukawa, and mamaku. Other species have both Māori and English names, but it is the latter that is predominant, at least in my experience. Below are some such examples… Read more »
Posts tagged with Plants
Science Live: Secret World of Ferns broadcast
If you missed the live broadcast of Science Live: Secret World of Ferns, it's now on YouTube so you can watch it now or anytime you like. The video explores the surprisingly complicated life-cycle of ferns, how to distinguish the different kinds of tree ferns, and we see inside the room holding Te Papa's collection… Read more »
Subantarctic forget-me-not adventures
From the 23rd-30th of December 2013 I was given the opportunity to join Rodney Russ and his team at Heritage Expeditions on board the Spirit of Enderby for a week long adventure to the New Zealand Subantarctic Islands. Link to the Heritage Expeditions website with information about their scholarship The Heritage Expedition Trust awards several… Read more »
Recognition for Te Papa plant researcher
Congratulations to Phil Garnock-Jones on being awarded the Nancy Burbidge medal, for his longstanding and significant contribution to Australasian systematic botany. Phil is the first New Zealander to receive the award, which is the highest bestowed by the Australasian Systematic Botany Society. Systematic botany is the study of the relationships, naming, and classification of plants. Phil is… Read more »
Dotted landscapes in Aboriginal art
One of the sections in the current incarnation of the Ngā Toi, Arts Te Papa exhibition showcases a selection from Te Papa's collection of Australian Aboriginal art. The show Gifted: Aboriginal Art 1971 – 2011 includes Papunya Tula paintings created in the 1970's in a community near Alice Springs with a style of Aboriginal art… Read more »
The long-and-short of lycophytes
I don't do plant-free holidays, and one of the species I wanted to photograph during my recent Northland holiday was the tiny and rare Phylloglossum drummondii. This diminutive plant has a Nationally Critical conservation ranking, because of its low numbers and the destruction of its swamp habitat. Finding it necessitates a winter (or early spring) field trip,… Read more »
A sunny blue afternoon – making cyanotypes at Te Papa
A few years ago I was lucky enough to be given a jar of prepared solution to use to make my own cyanotypes. I work with the photography collection at Te Papa but I am not a photographer so I enjoyed this opportunity to experiment with making some photographs using an historic analogue process. Cyanotypes are a… Read more »
Re-planting New Zealand
In regard to Bob Brockie's recent article in the Dominion Post (24 June 2013, page A8), here is some rationale for viewpoints about plants that some commentators have teasingly called "eco-fascism". Instead, they are logical expressions about the conservation of New Zealand's biota and ecosystems, including their genetic integrity. For any effort claiming to be… Read more »
How to learn ferns
Last weekend I was out with the Kapiti-Mana branch of Forest and Bird, giving them an introduction to ferns. A few weeks back, I gave a similar walking-talk at Otari-Wilton's Bush in Wellington. Many people find ferns an appealing group to learn. Aside from their iconic status in New Zealand, good learning resources are available, and… Read more »
The small and the weedy: Foxton field trip
I spent a couple of days of the long weekend with the Wellington Botanical Society, exploring the Foxton area, between Whanganui and Palmerston North. Much of the first and second days were spent in the sand dunes between Himatangi and Foxton Beach, and at Koitiata near Turakina. Some surprising things can become weedy in the… Read more »


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Dr. Bonnie G. Metcalf School of Education
In today's global marketplace, the story's heroes will be educational leaders who are equipped to empower twenty-first century learners - both among youth in schools and adults in governments, businesses and the nonprofit sector.
The need is urgent. The rewards are great. If you're called to be an educational leader, the Bonnie G. Metcalf School of Education can prepare you for that calling.
CBU News
'The Apple Tree' opens CBU's 2015-16 theatre season
October 8, 2015
CBU's dean of engineering named Mayor's Innovation Honoree
October 7, 2015
5:30 pm - 7:00 pm at Yeager Center A111
October 22, 2015
October 22, 2015
5:30 pm - 7:00 pm at Yeager Center B111
November 19, 2015
November 19, 2015


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HN-PSA270 Hamilton Beach
Hamilton Beach 67800 HealthSmart Juice ExtractorItem # HN-PSA270
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About Hamilton BeachOne of the country's leading distributors of small kitchen appliances, Hamilton Beach Brands, Inc. sells over 35 million appliances every year. The company's most famous brands -- Hamilton Beach, Eclectrics, Proctor Silex, and TrueAir -- are found in households across America, Canada, and Mexico. Hamilton Beach takes immense pride in their product quality, wide variety of options, superior customer service, and brand name strength and remains commi


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Bloomfield Road, Tipton, DY4 9EB
3 bedroom homes
£157,500 - £168,995
Barrows Place is a popular development in Tipton, close to a wide range of everyday amenities in the lively town of Dudley. We have a great selection of homes at Barrows Place which will appeal to you and your family's needs. Our homes have been designed for the way you want to live. Flexible layouts maximise on natural daylight and lovely open-plan rooms give you all the space you need.
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Ann Taylor has a special place in my closet, not just because of how much I love the quality of their pieces but also how my mom and I bond over and share these pieces. I'm so lucky that she passed her older Ann Taylor pieces to me (and I can wear them all the time without looking outdated). I recently picked up this shirtdress from Ann Taylor despite knowing I did not really need another dress in my closet, but I'm so glad I bought it: it's so comfortable for the summer (and would be cute for layering with cardigans once the weather gets cooler) and it's the sweetest shade of pink that gives a burst of color to my day. The only downside to this dress is that it tends to wrinkle, which is not a huge deal, but it can get annoying.
While it may be very hot here, I always bring a light jacket with me since I tend to get cold when I'm inside reading or studying. This cardigan from Forever 21 is the perfect transition piece, and it's so affordable. Granted, the fabric can easily snag on keys and bracelets, so it's not the best quality sweater. However, I love how light and airy it is for the summer, and how comfortable and stylish it is (especially paired with shorts because the differing lengths give a nice contrast and silhouette). Bonus: Forever 21 is having a huge sale on their website right now, and shipping is free no matter how much you spend!
I recently visited College Station with a couple of high school friends, and it was one of the most fun road trips. First of all, the food in College Station is A+. My favorite spot we tried was definitely Fuego (seriously, the best taco I've ever had). We also just had a great time being tourists around A&M (after a really intense rain storm that had us trapped in Fuego LOL). Good food, good friends, what more could I ask for?
Rompers are both one of my favorite clothing items and one of my least favorite. The first is obvious: they are effortless. You don't have to fuss with multiple pieces, and they are just so chic. However, let's be real– they are a nuisance getting on and off when nature calls. It's a struggle, but I have to say: I love this romper from Loft, and I am willing to deal with the struggle, lol. It's the most comfortable romper I own; it feels like I'm wearing my PJs in public. Plus, I love how classic the cut and print are (you know I love anything and everything with a cool print). I can't find the printed version online, but the black version is currently 40% off!
Rain has been in the forecast for days now due to Tropical Storm Bill, and I hope everyone is safe and well. Thankfully, the rain was more tame where I live compared to others, so my brother and I decided to be a little adventurous and test out more camera settings (since we are camera noobs). It was only sprinkling when we began to take some shots, but of course, it began to rain cats and dogs shortly after. However, some of the pictures turned out better than we thought they would (especially the ones with the fire hydrant LOL).
Hello, world!
I live in dresses all year round since they're so easy to just throw on, and there are so many beautiful dresses on Chicwish that I couldn't wait to share with you guys. My style is more girly, and I'm drawn to more feminine details like lace and embroidery (which I'm loving on the second dress along with the silhouette). I picked my favorites from the website, but there are so many more options of clothing at reasonable prices. Plus, if you sign up, you will get a 30% discount, so check out the website!
Hello, world!
It's not officially summer yet, but (surprise, surprise) the temperature hit 100 degrees yesterday. While the temperature still continues to rise, I love cooling off in easy, breezy dresses. This dress from Joe Fresh is easily one of the most comfortable dresses I own, and it has by far the most interesting pattern on it. It's listed on the Joe Fresh website as the Kaleidoscope Dress, which is so fitting for such an intricate print. I've also been obsessed lately with my Old Navy sandals since they practically go with everything in my wardrobe, and they're a comfortable height for me.
Hello, world!
I know – I said I was back two weeks ago, but of course, my brother got sick and then I got sick. I'm finally feeling much better, and I'm finally able to write a new fashion post for you all. Summer is actually one of my least favorite seasons just because I get into a rut of just wearing t-shirts and shorts due to the Texas heat. However, this new trend of culottes are a perfect way of switching up my summer outfits and staying cool with how breezy and airy they are. Trust me, I was on the fence about this trend since they looked unflattering and they reminded me of elementary school (flashback to gaucho pant days), but the cut of the pants actually creates a pretty and feminine silhouette. I've found that I can pair these pants with nearly anything in my wardrobe, so be warned that I am definitely going to be featuring them many times in the future.


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|$29.34 & FREE Shipping
The Ecosavr Solar Fish are an effective liquid solar pool cover that greatly reduce heat loss and evaporation from your exposed pool surface. The small disposable plastic fish contain a Heatsavr liquid that is dispensed through a valve in the fin and is undetectable by swimmers. The safe and biodegradable liquid then forms a transparent layer on your pool to inhibit heat evaporation keeping your pool at a desirable temperature! One Ecosavr Solar Fish will cover 15,000 gallons, for approximately 25 - 30 days; Two Ecosavr Solar Fish are required for larger pools.
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I haven't noted any temperature increase when used as directed in a 30x16 pool.Published 3 months ago by Cindy Vitale
This seems to work. I had trouble getting it to leak the solution out without developing bubbles, but I seem to have solved it now.Published 4 months ago by John Moore
left them in skimmer but thermometer did not change even one degreePublished 4 months ago by Clarence W Ivy
Don't know why or how but these actually keep the heat in your pool, not as efficient as an actual cover, but certainly much less hassle and less costly.Published 7 months ago by FRANCES E SHARP
Can't really tell if it works or not. Recommended by the Pool installer.Published 9 months ago by Speedy88
Still love the idea of these fish, but the weather's been so poor this summer it has been hard to determine how well they work. Jury's out on this one!Published 14 months ago by Linda A. St George


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The Library's collection of new and favorite games include a wide variety of genres: racing, role-playing, sports, and arcade games.
You'll never be bored. Check back often to discover new titles.
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Rogers family
Rogers family.
The ancestors and descendants of Dr. David Rogers, by Edward Francis Fremaux de Beixedon, jr.
Early lands of the Rogers family in the Foxtown area (Millington Society) of East Haddam, Connecticut / compiled by James Allen Rogers of Centennial, Colorado, with assistance from Dr. Russell I. Haley.
The Norcross family of Henry County, Missouri and related families : Rogers, Walker, Tarter, Swift, Keltner, Lancaster, Beers, Catron, Pinnell, Jones : a genealogical record and historical account of Calvin and Gladys (Rogers) Norcross of Henry County, Missouri, and their ancestors and descendants / Claude E. Norcross.
Rogers genealogy, Charles Thomas Rogers and descendants, compiled by Lewis G. Rogers and Wilmot Polk Rogers.
Rogers; some of the descendants of Giles Rogers, an immigrant to Virginia in 1664 ... including the family names of Barksdale, Croghan, Gatewood, George, Gwathmey, O'Fallon, Temple, Thruston, Tyler, Underwood, etc., compiled as the first step towards a chart by Hopewell L. Rogers ...
Rogers, Ward, Shipman, and allied families, by Harold I. Meyer, Mrs. Sylvan L. Mouser [and] Mrs. Voris R. Norton.
Rogers family--Homes and haunts--Connecticut--East Haddam (Town)--Registers.
Early lands of the Rogers family in the Foxtown area (Millington Society) of East Haddam, Connecticut / compiled by James Allen Rogers of Centennial, Colorado, with assistance from Dr. Russell I. Haley.
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G.Skill Releases Flare Series DDR3-1800 MHz Memory for AMD AM3 Platform
G.Skill Releases Flare Series DDR3-1800 MHz Memory for AMD AM3 Platform
G.Skill International Co. Ltd., manufacturer of extreme performance memory and high performance solid-state storage, has today launched its latest Flare series high-performance DDR3 kits rated at 1800MHz, and optimized for AM3 790 & 890 platform with AMD C3 stepping processors.
G.Skill's Flare series has been specifically designed and tested for AM3 790 and the latest 890 platforms, in combination with AMDs recent C3 stepping CPUs, and has passed a strict testing regime to ensure the best performance and stability. G.Skill Flare provides AMD enthusiasts the perfect choice of high frequency memory that allows a greater attainable CPU bus clock, in order to overclock further and have best performance for productivity and gaming.
The new Flare series DDR3 kits consist of the following specification:
DDR3 1800MHz 4GB (2GBx2) CL6-8-6-24 1.65V
DDR3 1800MHz 4GB (2GBx2) CL7-8-7-24 1.65V
DDR3 1800MHz 4GB (2GBx2) CL8-8-8-24 1.65V
DDR3 1800MHz 4GB (2GBx2) CL9-9-9-27 1.65V
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Yes Mattel design has a reputation for successfully fixing the 4H's mistakes.:hmlol:
Type: Posts; User: Grayskull's Heir
Yes Mattel design has a reputation for successfully fixing the 4H's mistakes.:hmlol:
Same here. I can't paint. Still I thought it was an easy fix.
Mattel is never going to get the color right anyway. The 200x head pack proves this.
Exactly! After the PD Teela situation, it's no wonder people feel like Mattel is hiding something. I can't see myself subbing up unless they show an early production sample of at least one figure.
I definitely need Superman because he was in the vintage DC comic with He-Man!
I have no need for Batman though
I know. That's why I said that with combined shipping the January (CC) figure will have to be held till February and then ship together with the February HMATMOTU figure. :hmgrin:
Yes it's a bit off but I can't really put my finger on what the problem is. It looks just like Alcala's art but it doesn't blend well with the Classics figures. It's way too expensive anyway (around...
I agree with this!
But I'm sure we'll see a year 2. these are very attractive designs and the price is a very important selling point. Personally, I don't want another collection of 150 or more...
Why does everyone insist that the bi-monthly subs can't combine? I don't get it. What am I overlooking that makes it impossible to ship the January CC figure together with the February HATMOTU figure...
By that logic we shouldnt be doing the ranking poll at all. How is ranking the the 35 not boosting the "losers" (a term I find completely inappropriate in this context) up? Whoever is on position 35...
It's becaue Dan is so good looking. Put him next to CG and it looks ugly :hmlol:
Please reread my post and the example I gave before making fun me. Your comment is completely unrelated to the point I was trying to make.
- - - Updated - - -
Thanks for the explanation...
They didn't.
They promised they would look into it and address it in time but they didn't.
Of course they could combine them easily. The two bi-monthly subs could be combined into six shipments...
I completely agree!
I don't know if my questions got overlooked, sorry for asking again:
Have we seen samples of the packaging? (Apart from the SDCC slides)
Is it confirmed that the packaging is...
Not trying to attack you, but how does what you are saying in your post have anything to do with what I said?
I've clearly said that I love these polls and have thanked the organisers,...
This is something we should discuss further. JVS3 and AdultCollector, this is an important point to consider IMO.
This voting system forces you to put characters in your personal top 35 that you...
HATMOTU is a variant sub. I don't think another all-variant sub on top of HATMOTU would sell.
Wow so all the great stuff is in round 2? :hmlol:
:hmlol: I was typing this last night on my iphone and was too tired to look!
I'm trying to rank the list right now but it's not easy.
I'm not happy with some chopices but above all several...
Have pictures of the final packaging been posted? (I'm not talking about the SDCC slides)
Has it been confirmed it's resealable?
I'm not impressed. The proportions of the different body parts are way off. Look at those forearms! They're way too long. Look at those hands: way too big. Skeletor's face: too small.
I hope this is...
Bubblor made the list but Rajar and Major Header didn't?
Are these polls still representative? Are enough people voting?
I'm really curious what the final results will be and how they...
I don't think I'll buy my usual two subs this time. I might give in and buy one, but I haven't decided yet.
The good:
the price
The bad:
Awesome review! I thought it was really great that Dan clearly said what is wrong with the figure instead of just presenting it. I loved this.
I think this set still is pretty good. And as I...
But he is a full repaint with a new head :hmeek:
An accessory set won't make it possible to make a proper version of that character.
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Sorry if I keep insisting in this (I guess I come across as annoying, whiny and ungrateful, which is not the case! I love these polls! they're awesome!), but this is precisely the reason why I think...
It will go well with his cup of wine :hmlol:
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In this case I really think these two characters should have been included in these polls. I think this is situation quite...
Agreed! These polls are awesome! Thanks for the hard work!
Has it been officialy confirmed by Mattel that Sagitar and the 200x Centaur Chieftain will take up beast slots? I don't think either of...
There's a poll in the poll section. Roughly 75% want him regular or "Ram-Man-Sized" (the $30 slot) and only very few people want him to take a "beast" slot.
beast slots are too rare and valuable.
I like orsogranito's version!
I think it's way too big and out of scale. It's a mechanical horse, not a tank. Imagine how off it will look next to Caslte Grayskull.


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Torino have edged closer to signing Djamel Mesbah after meeting with Milan officials on Tuesday.
Toro President Urbano Cairo held talks with Diavolo Vice-President Adriano Galliani earlier today to discuss the Algerian.
Reports suggest that the left-back, now 27, will join the newly promoted outfit in a co-ownership agreement.
Mesbah only signed for the San Siro giants in January from Lecce. He scored one goal in 12 games for Massimiliano Allegri's side.
Check out OLBG.com's list of ££ Free Bets from UK bookmakers before placing your bets!


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Michael Jackson Tribute Show Moonwalker: The Reflection of Michael
* Additional fees apply. No coupon or promo codes necessary to enjoy the displayed discount price.
The last date listed for Moonwalker: The Reflection of Michael was Saturday January 19, 2013 / 8:00pm.
Currently at California Theatre of the Performing Arts
Entrancing Musical Phantom From the Creators of Tony-Winning Nine
- Full Price:
- $67.50
- Our Price:
Before the Broadway version was even a glint in Andrew Lloyd Webber's eye, a musical adaptation of… More
Venue Details
Reviews & Ratings
Featured review from Goldstar Member
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Excellent Performance! Michael Firestone was great!
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OH MY GOODNESS!!! This show was absolutely excellent! If you are any type of a Michael Jackson fan this show is a must see. Needless to say I highly recommend this show and it is very suitable for children and adults of all ages.
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Terrific show. Brought all the nostalgia of watching Michael Jackson live in concert. (except I would never have had such terrific seats) the singing and choreography were on-the-dot precise. Amazing! We had no idea it would be so emotional for...continued
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Very entertaining. Excellent moves by dancers and vocals were great. Would like to see this show again.
Quotes & Highlights
"Never before has a full production show ever come this close to the real thing." —The Arcada Theater
"Simply astounding." —Los Angeles Times