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Methyl-H3K9-binding protein MPP8 mediates
E-cadherin gene silencing and promotes tumour
cell motility and invasion
Kenji Kokura1, Lidong Sun1,
Mark T Bedford2and Jia Fang1,*
1Department of Molecular Oncology, H. Lee Moffitt Cancer Center and
Research Institute, Tampa, FL, USA and2Department of Carcinogenesis,
The University of Texas MD Anderson Cancer Center, Science Park-
Research Division, Smithville, TX, USA
H3K9 methylation has been linked to a variety of biologi-
cal processes including position-effect variegation, hetero-
chromatin formation and transcriptional regulation. To
further understand the function of H3K9 methylation, we
have identified and characterized MPP8 as a methyl-H3K9-
binding protein. MPP8 displays an elevated expression
pattern in various human carcinoma cells, whereas
knocking-down MPP8 results in the loss of cellular
mesenchymal marker as well as the reduction of tumour
cell migration and invasiveness, suggesting that MPP8
contributes to tumour progression. Following characteri-
zation demonstrates that MPP8 targets the E-cadherin
gene promoter and modulates the expression of this key
regulator of cell behaviour and tumour progression
through its methyl-H3K9 binding. Furthermore, MPP8
interacts with H3K9 methyltransferases GLP and ESET,
as well as DNA methyltransferase 3A. MPP8 knockdown
decreases DNA methylation on E-cadherin CpG island
attended by the loss of DNMT3A localization, indicating
MPP8 also directs DNA methylation. Together, our results
suggest a model by which MPP8 recognizes methyl-H3K9
marks and directs DNA methylation to repress tumour
suppressor gene expression and, in turn, has an important
function in epithelial-to-mesenchymal
The EMBO Journal (2010) 29, 3673–3687. doi:10.1038/
emboj.2010.239; Published online 24 September 2010
Subject Categories: chromatin & transcription; molecular
biology of disease
Keywords: chromodomain; DNA methylation; EMT; H3K9
methylation; transcription
Carcinogenesis is a progression of events resulting from not
only the accumulation of genetic alterations but also the
disruption of epigenetic modifications (Yoo and Jones,
2006). In eukaryotic organisms, the epigenetics network has
many layers of complexity that could be summarized in four
major modifications: DNA methylation, histone modifica-
tions, chromatin remodelling and microRNAs (Esteller,
2006). As one of the key players in the chromatin regulation,
histone octamers are wrapped around with 147bp of DNA to
form a nucleosome, which is subjected to at least eight
distinct types of post-translational modifications including
acetylation and methylation (Kouzarides, 2007). The complex
array of these modifications has been proposed to constitute a
'histone code' that can be recognized by different chro-
matin regulatory proteins, which in turn affect the chromatin
structure or regulate the accessibility of DNA to various
machineries (Jenuwein and Allis, 2001). In normal cells,
these modifications are delicately balanced, and small
changes in a given parameter can lead to major consequences
and ultimately result in cellular transformation and malig-
nant outgrowth (Ting et al, 2006).
Epigenetic silencing of tumour suppressor genes is a
common event during carcinogenesis and often involves
aberrant DNA methylation and histone modifications (Wang
et al, 2007). One well-studied example is the transcrip-
tional repression of the pleiotropic cell behaviour regulator
E-cadherin. Emerging evidences indicate that epigenetic silen-
cing of E-cadherin during tumour progression is achieved by a
combination of different mechanisms, including transcription
factors, promoter hypermethylation, histone deacetylation
and methylation (Peinado et al, 2007). For example, zinc-
finger transcription factor SNAIL recruits H3K27 HMTase
polycomb repressive complex 2 (PRC2) and Sin3A/HDAC
complexes to repress E-cadherin expression (Peinado et al,
2004; Herranz et al, 2008). DNA-binding proteins ZEB1/2
and several chromatin modifying enzymes co-exist in the
transcription co-repressor CtBP-1 complex to downregulate
E-cadherin expression (Shi et al, 2003). In addition, promoter
hypermethylation has been associated with E-cadherin gene
silencing in various carcinoma cells (Grady et al, 2000).
Although the interplay between DNA methylation and his-
tone modifications has been well-documented, the molecular
details of how these mechanisms cooperate for E-cadherin
gene repression remain unclear.
As one of the best-studied histone modifications, histone
methylation occurs on both arginine and lysine residues and
can be recognized by effector proteins harbouring different
methyl-histone-binding domains (Kouzarides, 2007). So far,
six distinct motifs including chromodomain, Tudor domain,
WD40 repeat domain, MBT domain, PHD domain and ankyr-
in-repeats domain, have been shown to be recruited by
different methylated lysines on histone tails and this recruit-
ment is a critical step for the functional consequences asso-
ciated with different methylation events (Taverna et al, 2007;
Collins et al, 2008). The first identified example of methyl-
lysine-dependent protein–protein interaction was between
H3K9 methylation and heterochromatin protein 1 (HP1).
Received: 28 April 2010; accepted: 30 August 2010; published online:
24 September 2010
*Corresponding author. Department of Molecular Oncology, H. Lee
Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive,
Tampa, FL 33612, USA. Tel.: þ1 813 745 6716; Fax: þ1 813 745 7264;
E-mail: firstname.lastname@example.com
The EMBO Journal (2010) 29, 3673–3687|& 2010 European Molecular Biology Organization|All Rights Reserved 0261-4189/10
&2010 European Molecular Biology OrganizationThe EMBO Journal VOL 29|NO 21|2010
Page 2
HP1 proteins recognize methyl-H3K9 through its N-terminal
chromodomain and form a protein dimer with a wide range
of chromosomal proteins through its C-terminal chromo-
shadow domain for various functions including heterochromatin
formation, telomere capping and transcriptional regulation
(Kwon and Workman, 2008). Although methyl-H3K9 is one
of the major repressive marks and HP1 proteins have been
co-purified with several repressive protein complexes (Ogawa
et al, 2002; Shi et al, 2003), both HP1g and H3K9me3 also
associate with the coding regions of a number of active genes
and their presence relies on elongation by RNA pol II (Vakoc
et al, 2005). Therefore, the functional outcomes of H3K9
methylation are determined by their localization in chromatin
context and accessibility to different methyl-histone-binding
To determine whether other effector proteins are also in-
volved in mediating biological functions of H3K9 methylation,
we performed an in vitro screen using a protein chromodomain
microarray. We have identified an uncharacterized protein
MPP8, which is capable of recognizing methylated H3K9
marks through its chromodomain in vitro and in cells.
We also demonstrate that MPP8 represses E-cadherin gene
expression and is involved in regulation of tumour cell
growth and epithelial-to-mesenchymal transition (EMT)
through methyl-H3K9 binding. Biochemical analyses reveal
that MPP8 associates with H3K9 methylation and DNA
methylation machineries and co-localizes in E-cadherin pro-
moter region. Importantly, MPP8 also directs DNA methyla-
tion by recruiting DNMT3A to the 50-regulatory regions of
E-cadherin gene. Together, our work not only characterized
MPP8 as a methyl-H3K9-binding protein, but also revealed a
novel molecular mechanism by which MPP8 couples histone
H3K9 methylation and DNA methylation for tumour suppres-
sor gene silencing and metastasis.
MPP8 chromodomain is a methyl-H3K9-binding motif
The fact that different methyl-binding proteins can be re-
cruited to same or different methylated lysines to mediate
functional outcomes indicates that the translation of the
'histone code' is more complex. For example, methyl-H3K9
recruits chromodomain containing proteins HP1 and CDY
(Fischle et al, 2008; Kwon and Workman, 2008), as well as
the ankyrin-repeats containing protein G9a (Collins et al,
2008), whereas the WD40 domain containing protein EED
recognizes both H3K9me3 and H3K27me3 marks (Margueron
et al, 2009). To explore the possibility that additional chro-
modomain containing proteins possess the ability to 'read'
the H3K9me marks, we generated a protein chromodomain
array, which contains 16 representative chromodomains
fused with GSTand screened their binding ability to different
methyl-H3 peptides as described previously (Kim et al, 2006).
As expected, chromodomains from HP1 proteins recognize
H3K9me2 and H3K9me3 peptides, but not the unmethylated
control (Figure 1). Furthermore, we observed that H3K9me2
and me3 peptides also bind to the chromodomain of an
uncharacterized protein MPP8 (arrows in Figure 1), which
was initially isolated in an expression-cloning screen using an
antibody against M-phase phosphoproteins (Matsumoto-
Taniura et al, 1996). To further determine the specificity of
these interactions, we re-probed the same array with H3K4me3
and H3K27me3 peptides. None of the arrayed chromodomains
showed obvious binding to these peptides (Figure 1).
MPP8 binds to methylated H3K9 in vitro and in vivo
Different from HP1 proteins, MPP8 is a relatively large
protein (860aa) and does not have homologs in Drosophila
melanogaster and Caenorhabditis elegans, suggesting that
MPP8could functionthrough
Domain structure analysis reveals that MPP8 contains one
chromodomain near the N-terminal, with four copies of
ankyrin-repeats near the C-terminal (Figure 2A). In vitro
translation coupled peptide pull-down assays demonstrate
that full-length (FL)-MPP8 is still capable of binding to
immobilized methyl-H3K9 peptides (1–20aa) but not the
unmethylated form (Figure 2B, upper panel). Furthermore,
we mutated a conserved aromatic residue in MPP8 chromo-
domain (W80A) for same binding assays. As indicated in
Figure 2B, this single amino acid substitution completely
abolished MPP8 binding ability to same methyl-H3K9 pep-
tides (middle panel). As control, HP1a showed the similar
methyl-H3K9-binding profile, which is consistent with
previous reports. To better mimic the in vivo situation, we
also chemically installed different methyl-H3K9 analogs on
H3 (Simon et al, 2007) for the in vitro pull-down assays and
observed a similar methyl-H3K9-binding profile of MPP8
(Supplementary Figure S1A). Together, these results indicate
that the chromodomain is critical for methyl-H3K9 binding of
MPP8 in vitro even though the ankyrin-repeats domain has
beenshown asanothermethyl-lysine-binding
(Collins et al, 2008). In addition, we examined whether
Figure 1 The MPP8 chromodomain is a methyl-H3K9-binding motif. The chromodomain array was probed with Cy3 labelled H3K9me2,
H3K9me3, H3K4me3, H3K27me3 and H3 (1–18) peptides and a-GST, as indicated. A key to the arrayed chromodomains is given together with
accession numbers. The middle position (grey circle) contains GST alone as a background indicator. Arrows indicate binding of MPP8
chromodomain to H3K9me2 and me3 peptides on the array.
An H3-methyl-K9-binding protein
K Kokura et al
The EMBO JournalVOL 29|NO 21|2010
&2010 European Molecular Biology Organization
Page 3
MPP8 recognizes H3K27me marks because of the high
sequence similarity between K9 site and K27 site (Min et al,
2003). In the same assays, MPP8 displayed no binding to
either H3 peptides harbouring methylated or unmethylated
K27 (18–37aa) (Figure 2C) or H3 chemically installed with
different methyl-K27 analogs (Supplementary Figure S1B).
Collectively, these data clearly demonstrate that MPP8 speci-
fically binds to methyl-H3K9 in vitro and its binding ability
requires an intact chromodomain.
H3K9me3 is enriched in pericentric heterochromatin
regions where DAPI heavily stains (Rice et al, 2003). We
thus expect MPP8 to be localized in these regions if MPP8
recognizes H3K9me3 marks in cells. In this regards, we
expressed Flag-MPP8-FL or its W80A mutant in immortalized
MEF cells. Immunostaining results indicate that Flag-MPP8
wild-type (wt) localizes on heterochromatin where H3K9me3
marks are enriched, whereas the W80A mutant uniformly
distributes in the nucleus (Figure 2D, upper and bottom
panels). These results in combination with our peptide
pull-down data suggest that MPP8 localizes on hetero-
chromatin through H3K9me3 binding. To test this possibility,
we expressed Flag-MPP8-wt in immortalized MEF cells
derived from Suv39h1/h2 double knockout mice (Peters
et al, 2001). Similar to the previous reports, depletion of
Suv39h1/h2 led to the loss of enrichment of H3K9me3 in
heterochromatin regions (Figure 2D, column 2). Importantly,
loss of heterochromatin localization of H3K9me3 is conco-
mitant with a loss of MPP8 localization on heterochromatin
(Figure 2D, middle panels). Given that the expression
of Flag-MPP8-W80A mutant in MEF cells does not affect
Figure 2 MPP8 binds to methylated histone H3K9 through chromodomain. (A) Diagram of full-length MPP8 and chromodomain alignment
among human MPP8, HP1a and PC2. Conserved amino acids are shaded. The conserved W80 is indicated with a star. (B, C) MPP8 specifically
recognizes methyl-H3K9 in vitro.35S-labelled in vitro translated MPP8-wt or W80A mutant were tested for binding with immobilized biotin-H3
peptides with or without methylation at K9 (B) or K27 (C). Methylation states are indicated.35S-labelled in vitro translated HP1a serves as
controls in the parallel.'In' represents 10% of total input,'B' and 'Ft' represent bound and flow through fractions, respectively. (D) H3K9me3
marks recruit MPP8 onto heterochromatin in cells. Immortalized MEF cells derived from wild-type or Suv39h1/h2 knockout mice were
transfected with vectors expressing Flag-MPP8 or W80A mutant. Cells were fixed and stained with mouse anti-Flag (red) and chicken anti-
H3K9me3 (green) antibodies. Blue colour (DAPI) shows the nuclei. (E) MPP8 preferentially binds to chromatin containing H3K9me2 and me3
marks in cells. MPP8 stable knockdown 293T cells (KD2) were transfected with vectors expressing shRNA resistant Flag-MPP8 or W80A
mutant. MPP8 and bound chromatins were IPed by anti-Flag antibody and analysed by western blot. Antibodies are indicated and a non-
specific cross-react band is labelled with a star.
An H3-methyl-K9-binding protein
K Kokura et al
&2010 European Molecular Biology OrganizationThe EMBO JournalVOL 29|NO 21|2010 3675
Page 4
distribution of H3K9me3 on heterochromatin (Figure 2D,
bottom panels), these results demonstrate that MPP8 recog-
nizes H3K9me3 marks in cells.
To further explore MPP8 functions, we generated MPP8
antibody that specifically recognizes a protein band with
molecular weight about 110kDa from whole cell lysates on
western blot (Supplementary Figure S2A). To validate that
this protein band indeed represents the endogenous MPP8,
we generated two MPP8 stable knockdown 293T cell lines
using vector-based short hairpin RNA (shRNA) targeting to
different regions of human MPP8. Western blot analysis not
only confirmed the antibody specificity but also indicates that
over 90% knockdown efficiency has been achieved with both
shRNAs at the protein level (KD1 and KD2; Supplementary
Figure S2B). Next, we rescue expressed shRNA resistant
Flag-MPP8-wt or W80A mutant in MPP8-KD2 293T cells.
Flag-MPP8 was then immunoprecipitated (IPed) and the
MPP8 bound nucleosome was analysed by western blot
using specific antibodies against different H3K9 methylation
states to determine the methyl-H3K9-binding preference of
MPP8 in cells. As indicated in Figure 2E, Flag-MPP8-wt was
able to pull-down H3 harbouring K9me2 and me3 marks but
not K9me1 or K4me2 marks (lane 6), whereas the W80A
mutant was not co-purified with nucleosome (lane 7). These
results are consistent with our in vitro binding data as well as
immunostaining results, and indicate that MPP8 preferen-
tially recognizes di- and tri-methylated H3K9 through its
chromodomain in cells. We thus conclude that MPP8 is a
methyl-H3K9-binding protein.
MPP8 is important for tumour cell proliferation
Given that H3K9 methylation is critical for transcrip-
tional regulation and genomic stability (Peters et al, 2001),
we speculate that methyl-H3K9-bindingproteinMPP8
could have a function in cancer. To test this possibility, we
examined MPP8 expression in several human carcinoma cell
lines. As shown in Figure 3A, four of six breast cancer
cell lines showed an increased MPP8 expression compared
with immortalized human mammary epithelial cells hEMCs-
hTERT-LTand MCF10A (lanes 1–6, top panels). Additionally,
this elevated expression pattern was also observed in four of
six human non-small cell lung cancer cells (lanes 4–7, bottom
panels) and most of commonly cultured human tumour cell
lines including HeLa and U2OS compared with the normal
human fibroblast cell WI-38 (data not shown), implicating
that MPP8 may contribute to tumour cell maintenance.
To test this idea, we established two stable MPP8 knockdown
MDA-MB-231 cell lines using the same shRNA vectors we
described above. Western blot analysis indicates that over
90% knockdown efficiency has been achieved in both cell
lines (Figure 3B, top panels). Furthermore, MPP8 knockdown
leads to a moderate growth reduction in MDA-MB-231 cells
(Figure 3B, bottom panel), indicating MPP8 has a function in
tumour cell proliferation.
MPP8 promotes tumour cell motility and invasion
To further evaluate the functional outcomes caused by MPP8
knockdown, we performed the cDNA microarray analysis
using RNA generated from control or MPP8-KD2 MDA-MB-
231 cells. The raw data were analysed using SAM software
with R package as two class unpaired samples and the genes
with statistical significance between control and MPP8 knock-
down samples were further analysed on GeneGo MetaCoreTM
platform. Data mining and pathway analysis reveal that a
large group of dysregulated genes in response to MPP8
knockdown are involved in cell adhesion and EMT, implicat-
ing MPP8 has important functions in this process (Figure 4A).
Figure 3 MPP8 is important for tumour cell proliferation. (A) Western blot analysis of two immortalized human mammary epithelial cells
(MCF10A and hMEC-hTERT-LT) and six breast cancer cell lines (top panels); human normal fibroblast cells (WI-38) and six non-small cell lung
cancer cells (bottom panels). (B) MPP8 knockdown results in decreased cell proliferation. Top panels are western blot analysis of control and
two stable MPP8 knockdown MDA-MB-231 cell lines. Bottom panel shows the growth curve of different MPP8 knockdown cells. Viable cells
were quantified every 24h for 4 days after initial seeding. Cell numbers from six independent experiments were averaged, and the variations
are presented with error bars.
An H3-methyl-K9-binding protein
K Kokura et al
The EMBO JournalVOL 29|NO 21|2010
&2010 European Molecular Biology Organization
Page 5
In addition, pathway analysis also indicates that MPP8 could
be involved in translation and transcription regulations.
EMT is a highly conserved cellular process that has pivotal
functions in diverse processes during embryonic develop-
ment, chronic inflammation, fibrosis, and tumour progres-
sion (Thiery, 2002). To further investigate whether MPP8 is
indeed involved in EMT, we examined the epithelial
and mesenchymal cell markers in control and MPP8 knockdown
cells by western blot. MDA-MB-231 cells are typical metastatic
mesenchymal cells that express a high level of mesenchymal
marker Fibronectin, but not epithelial protein E-cadherin.
However, MPP8 knockdown results in a significant loss
of the Fibronectin expression and a marked increase of
E-cadherin expression (Figure 4B). In addition, differential
interference contrast microscopy analysis indicates that cells
changed their shapes to a cuboidal form in response to MPP8
Figure 4 MPP8 promotes tumour cell migration and invasion. (A) GeneGo statistical process analysis of DNA microarray data derived
from control and MPP8-KD2 MDA-MB-231 cells. Ten dysregulated GeneGo processes with best significance (lowest P-value) in response
to MPP8 knockdown are listed. (B) Western blot analysis using whole cell lysates derived from control and MPP8 knockdown MDA-MB-231
cells. Specific antibodies as indicated. (C) Scratch wounding assays on the confluent layers of control and MPP8 knockdown MDA-MB-231 cells.
Images were acquired at 0 and 24h after wounding. (D, E) Migration and invasion assays of control and MPP8 knockdown MDA-MB-231 cells.
Cells were induced to move or invade through uncoated or Matrigel-coated membranes for 12 or 24h, respectively. Membranes were then
fixed, photographed (D) or quantitated (E). Columns represent the mean of triplicate assays and the variations are presented with error bars.
Control cell numbers were normalized as 100%. P-values are indicated.
An H3-methyl-K9-binding protein
K Kokura et al
&2010 European Molecular Biology OrganizationThe EMBO JournalVOL 29|NO 21|2010 3677


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Camp Forest 2015 is instilling confidence building wilderness skills and widening cultural understanding. Thanks to Global Giving donors, more children who would not otherwise be able to attend have enjoyed a week at Camp Forest this summer. Especially special is the week dedicated to GIRLS!! Girl's week was an enormous success. Girls will often "allow" the boys to dominate the more active and challenging skills. But when there are ALL GIRLS, they step up and give it a try.
The Watersheds Expedition, August 24 – 28 is still accepting enrollments. A child and/or parent can come from anywhere in the world to enjoy Maine's primitive beauty. So send a child you know or donate to assist a child you don't.
Join us for this wonderful expedition! We'll spend five whole days exploring the watersheds of the Passagassawaukeg River and the Marsh Stream in Brooks, Maine. This expedition begins at Camp Forest on Monday morning, where we'll learn how to use our gear and pack up for the expedition. After practicing with the gear and packing it all up, we set off on our first hike to Half Moon Pond to set up a comfortable primitive campsite and canoe and fish on the pond. We can visit our friends the beavers, muskrats, eagles, and fox. Who knows what you'll catch sight of!? From there we will hike up a mountain where we can see the entire Passagassawaukeag watershed from Brooks to Belfast Bay and see sail boats on the harbor. From there we will travel to the Marsh Stream watershed. We'll end the week traveling down the Marsh River to the Brooks Park. During this expedition, skills for the Osprey, Eagle, Beaver and Maine Woodsman will be learned. If you're ready for an amazing wilderness camping experience, this expedition is for you! Ages 8 and up, if ready.
Camp Forest 2015 schedule is up on line. Take a look and see how exciting this ecological wilderness day and expedition camp is! Among the new offerings this year are Earth Girl's Camp – an entire week of Day Camp just for girls and Beaver Skills Week - a week of advanced wilderness skills. Returning for campers from anywhere in the world is the week long expedition down the Passagassawakeag River in Waldo County, Maine. This river offers slow moving lazy travel through field and forest, white water approaching the salt water of Belfast Bay.
Research has shown the many advantages of getting out into nature, Richard Louv wrote of the need for children to unplug and the healing nature of the outdoors for ADHD and other issues with plugged in kids. So help unplug children who might not otherwise be able to attend a summer camp. Your donation goes directly to pay a partial or full campership. THANKS as we look forward to a GREAT SUMMER
Camp Forest 2014 was a great year. With several very well trained staff and volunteers we were able to reach many children this year. Teaching them about the great outdoors and mentoring in community and nature. We were able to give opportunities to children who are very interested in learning more about the natural world, to come to several weeks of camp that they normally couldn't afford. We were able to give children who would never be able to afford any camp experience, a highly trained staff, and one on one immersed in nature and Camp Forest educational program. Some children have continued to come back to Camp Forest from 7 years old to 17. With help from our scholarship program at Camp Forest their interest has been fostered even when their families have hard economic times, Camp Forest is still there for them. Some children have attributed Camp Forest to their interest in higher college education in environmental studies and wilderness emergency medicine. Our expert staff at Camp Forest is able to mentor children who are very interested and great learners, as well as children who have many emotional needs and parents that are not around for them. We are there for them. And we wish to continue to be able to mentor children at Camp Forest from all economic classes and needs. That is why we need our scholarship program at Camp Forest so we can continue to serve those in the area who really need the help, and would not otherwise be able to afford to have this experience that can help them in their very important childhood years. Thank you for supporting Camp Forest Scholarship Program. Sincerely Adam Stone at Camp Forest.
Maine has many forests, lakes and streams, and many Summer Camps. Camp Forest is a unique camp. Not only is our programming a full immersion experience in the forest, but also integrates wilderness education, interpersonal relationships and psycho-emotional healing in all the activities. Camp Forest also has the mission to offer this camp experience to low income children. Through the assistance of donors, finances have not been a barrier to camp attendance.
Children of parents limited by physical ailments, opiate addictions, or family tragedy are not uncommon. These children are not often given an opportunity to heal, or experience how the natural world can help them. Holding a frog in her hand and seeing its life, learning to hike and be strong to overcome physical restrictions, spending time playing and being happy brings the opportunities to confidently face life.
Camp Forest has provided a bit of stability for children who are forced to sleep in the vehicle with their single mother, or those with unstable home lives. Occasionally these children would run off and cry to a tree for a while, then return ready to face the world.
We have watched children who have problems with school, gain interest and a passion for learning at Camp Forest. We have watched children who would not listen, grow into amazing, attentive students.
Our low camper to staff ratio, un -walled free learning style, coyote teaching techniques, respect for all life, and peer mentoring is designed to find a child's own inner passion for learning. We have successfully blended these children with high functioning children from healthy and skilled families.
If you want to help us make a difference in a child's life, Step up and Do it.
Thank you.
Adam Stone, Senior Counselor
(lightly edited)
Project Reports on GlobalGiving are posted directly to globalgiving.org by Project Leaders as they are completed, generally every 3-4 months. To protect the integrity of these documents, GlobalGiving does not alter them; therefore you may find some language or formatting issues.
If you donate to this project or have donated to this project, you will get an e-mail when this project posts a report. You can also subscribe for reports via e-mail without donating or by subscribing to this project's RSS feed.
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Neoseeker : Games : Nintendo 64 : Classic & Puzzle : Puzzle Games : Mario Party
BackgroundA four-player party game featuring popular Nintendo characters: Mario, Luigi, Princess, Donkey Kong, Bowser, Yoshi, and Wario. There are over 50 mini-games including racing, skill and puzzle games, and 8 different boards to play on.
Features- Up to 50 mini-games
- 6 playable characters
-6 boards to play in
-Buy items at the item shop, such as blocks, music etc.
- Mini game island. A place where you have a challenge to beat a number mini games
-For 1-4 players
Official URLOfficial Site
Recent threads
Chiptune and 135 others own Mario Party
Militron and 57 others played Mario Party
KurtisTrentOn Tuesday, December 10th, 2013, I discovered the Eternal Star Stage. I did not master this game until that very day. MarioParty N64
Dec 27, 13 4:01pm
PichuRulesA good start to the series, I love this game MarioParty N64
May 15, 13 6:05pm
Fluidityadded 12 new screenshots
Apr 09, 12 1:44pm
mariokemonadded a cheat
Jun 26, 11 4:31pm
Sonic_2Good game and the most difficult Mario Party. MarioParty N64
Jul 19, 10 4:20pm
deathreaper82Its the first one! MarioParty N64
Jul 19, 09 3:27pm
SlashdragonOriginal Mario Party was a winner. MarioParty N64
Apr 04, 09 11:29am
MattchuLBThis game crafted some amazing memories for me. MarioParty N64
Feb 01, 09 8:27am
The wolf nmd Alexfun for d whole family!!! ***** MarioParty N64
Jan 19, 09 2:05pm
Dec 07, 08 10:25am
Nov 15, 08 5:39am
Woo! Party!
Released in 1999 by Nintendo, Mario Party was Nintendo's attempt at a party game....
smash mario55exciting game for anyone ! MarioParty N64
Sep 19, 08 4:05am
Aug 08, 08 2:04pm
SpiritualA simple game with great minigames but a terrible audio repository. MarioParty N64
Aug 02, 08 1:44pm
Lost SoulCompleted MarioParty N64
Jul 10, 08 1:24pm
lil nudistgirlCompleted MarioParty N64
Jul 03, 08 10:11am
May 10, 08 5:53pm
GameTester113Beaten MarioParty N64
Apr 14, 08 4:06am
- Genre: Puzzle Games (N64)
- Theme(s): Party & Board Games
- Perspective(s): Third Person
- Developer: Hudson Soft
- Publisher: Nintendo
Released North America Feb 8, 1999 Europe Mar 9, 1999 Japan Dec 18, 1998
- Player Info:
- Offline Multi-player (4 max)
Mario Party Series
NeoWiki Pages
Mario Party


text: When you're looking at your options for your future, there are a lot of factors to keep in mind. It's time to look at all of them and weigh which ones are most important to you. Assess Your Personality & Preferences Broaden your search to include sectors that you may not have previously considered. Learn more about your your interests and how they would fit with a particular career. Strong Interest Inventory Myers-Briggs Type Indicator Life Values Inventory Career Exploration Guiding Questions Research Industry For each career field that interests you, do some research and connect with individuals in the field to truly understand that job or industry. Recent Alumni Data Connect With Professionals Industry Research Gain Experience Participate in on-grounds activities, student organizations, leadership and service programs. Student Organizations Visit the Career Center Meet with a Career Counselor Connect with Alumni


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Maybelline SuperStay 24HR Makeup - New Version
617 reviews
58% would repurchase
Package Quality: 2.9
Price: $$
Package Quality: 2.9
Price: $$
INGREDIENTSAqua/Water, Cyclopentasiloxane, Polypropylsilsesquioxane, Isododecane, Dimethicone, C30 45 Alkyl Dimethylsilyl Propylsilsequioxane, Glycerin, Silica, PEG 10 Dimethicone, Dimethicone/Polyglycerin 3 Crosspolymer, Sodium Chloride, Nylon 12, Phenoxyethanol, Disteardimonium Hectorite, Disodium Stearoyl Glutamate, Chlorphenesin, Ethylparaben, Disodium EDTA, Acrylates Copolymer, Aluminum Hydroxide, Paraffin, May Contain ( /-): CI 77891 (Titanium Dioxide), CI 77499, CI 77492, CI 77491 (Iron Oxides)
on 5/24/2014 3:00:00 AM
Age: 19-24
Skin: Sensitive, Olive, Not Sure
Hair: Brunette, Wavy, Medium
Eyes: Hazel
On the plus side this foundation has great coverage, and although I'd question the 24 hour claim it lasted throughout several 8 hour days without touch ups. Unfortunately, the cons outweighs the pros. First and foremost, this foundation made my relatively clear skin break out terribly; I'm not talking a few pimples, I'm talking about 20 over the course of the 3 weeks I used it. As always the lack of pump caused a few issues. I also found the finish of this, even with a thin layer, to be on the cakey side. I have no issue with a matte finish but it almost looked powdery. If I were to ever use it again it would maybe be for a night out when I want that full coverage effect but it's definitely too clogging for regular use.
on 4/28/2014 6:01:00 PM
Age: 30-35
Skin: Normal, Fair, Not Sure
Hair: Blond, Other, Other
Eyes: Hazel
Lovely foundation, is a matte finish and blends in nicely. My only issues are the colour range as I wish there were a few more choices and also I do find the colour oxidises to a shade or 2 darker. Originally I used my mac prolong wear concealer with this foundation and hated the result due both products being a matte finish, my skin was left looking very powdery and dry. I now use a lighter concealer With this foundation and the finish is very nice.
on 4/21/2014 4:55:00 PM
Age: 19-24
Skin: Very Dry, Medium, Warm
Hair: Brown, Straight, Coarse
Eyes: Brown
Shade: sand beige
- medium to full coverage
- nice staying power
- good for dry skin
- not great for when my skin decides to get SUPER dry
on 4/17/2014 3:42:00 PM
Age: 19-24
Skin: Combination, Medium, Not Sure
Hair: Brown, Other, Other
Eyes: Brown
For me, this foundation is a VERY close dupe for the MAC Studio FIx Fluid. I used the studio fix for many years, and i can honestly say i find the maybelline easier to blend and build. It works wonderfully, has insane staying power, and keeps my face looking matte all day. I also like to get a little bit on the back of my hand and mix equal amounts of it with the Maybelline Fit Me concealer to stipple in the areas where i have discolouration and need a little more coverage after the first layer of foundation is already on. The concealer helps it to build without getting cakey and dry, so for those of you who feel this product is hard to build without it looking cakey, try doing this and finishing it with a powder. You will adore this foundation even more!
on 3/18/2014 6:09:00 PM
Age: 18 & Under
Skin: Acne-prone, Fair-Medium, Not Sure
Hair: Black, Other, Other
Eyes: Brown
LOVE THIS FOUNDATION! This is probably one of the BEST drugstore foundations out there! It's gives you a flawless medium to full coverage and the finish of this foundation is FLAWLESS. It's matte and your skin just look soft. I have very oily, acne proem skin and this foundation doesn't control my oily. I get shiny about three-four hours late, but then I blot and re powder and it looks amazing! I love how this foundation doesn't look cakey at all like most drugstore foundation. The shade selection isn't really friendly. I got the shade natural beige and when I apply it, it's a perfect match, but after 1-2 hours later, it oxidizes and it looks like I have a fake tan on. I will go back and get the lighter shade, but over all, of you have normal skin, then you would LOVE this foundation!
on 3/13/2014 12:54:00 PM
Age: 19-24
Skin: Oily, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Black
It's alright but because I have oily skin I ind that it separates sometimes on my skin ..
on 3/6/2014 1:51:00 PM
Age: 18 & Under
Skin: Oily, Fair-Medium, Not Sure
Hair: Blond, Other, Other
Eyes: Brown
I admit, I LOVED this foundation! But It was turning orange on my face....I know this is my shade because I am not pale pale. Not one foundation am I the lightest shade, plus I am classic ivory in every other foundation from Maybelline. So around my lip area it looks like an orange foundation!! So horrible, This lasts maybe eight hours which is pretty good compared to others, and its pretty full coveraged, No pump..... So I am dumping it all over
on 2/23/2014 12:20:00 PM
Age: 18 & Under
Skin: Oily, Fair-Medium, Not Sure
Hair: Blond, Other, Other
Eyes: Brown
This foundation is not all that it claims to be. It is not, most importantly, 24 hour. Possibly my own fault for buying a foundation for normal skin knowing full well that I have oily skin but this really does not last any more than a few hours for me. As far as positives, it does give a flawless face to begin and it isn't over priced. I think this just isn't for my skin!
on 2/16/2014 12:55:00 PM
Age: 30-35
Skin: Combination, Tan, Not Sure
Hair: Black, Other, Other
Eyes: Brown
I bought this to replace my Cover Girl Outlast 3-in-1 foundation, since it was causing me to breakout. I don't think anything will replace that, but for around the same price point, this is close.
The colour matches my skin well, I'm around an NC42 and I got colour Honey Beige. The product blends very easily, but I find you need to do this quickly otherwise it starts to set. It doesn't keep me oil-free, nothing does, but it doesn't look cakey as the day goes on, nor does it when I first apply & set it with powder.
I'm not sure if I would buy it again, I may look for something else but so far its been pretty good.
on 2/10/2014 5:56:00 PM
Age: 25-29
Skin: Combination, Olive, Warm
Hair: Brown, Wavy, Fine
Eyes: Brown
- medium to full coverage
- long wearing
- looks natural
- doesn't oxidize
- satin finish
- no pump
- dries quickly
- feels heavy
- color selection
COMMENTS: I despise foundations with no pumps, but that's just how the cookie crumbles. This is a long wearing, satin finish foundation. Medium to full coverage. It stays put on my oily skin and I get a good 4 hours before I get oily, which is the norm for me. It still managed to stay on, but will get quite dewy the longer it's worn. The finish is comparable to L'Oreal True Match - satin (very natural) then gets quite dewy, except this one doesn't transfer and isn't as lightweight. I love how this makes my skin look, though. It really melts into the skin and even if a brush is used, it gives the appearance of having used a damp blending sponge as it doesn't sit on top of the skin or look unnatural. It also didn't oxidize, though I kind of wish it did because the color selection is very small. Also, because there's no pump you end up wasting a lot of product due to how long the bottle is. It's difficult to get all of it out. 'Classic beige' would be a perfect match if it leaned more yellow, but it's the closest I can get to my NC37 match.
Will repurchase.
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text:
Apple In-Ear Headphones (White)
" I don't know whether it's a conspiracy or a joke, but Apple's talk of these being good quality just makes them seem like hilarious liars who could sell ice to eskimos. If their intent is to drive aftermarket sales, then they've managed it. As far as I can tell these things literally don't play bass. It's just not there. The midrange is ok, and if you're tired, they're ok for watching movies..." --BBBS
Signature Acoustics O16 Live
" Signature Acoustics O-16 Live Review : Setting the Stage INDEX (Click to jump directly to sections): Introduction Packaging, Accessories and Comfort Sound Common sound impressions Let's do something about it/Mods Comparisons Value and Conclusion Introduction: ..." --getclikinagas
Apple EarPods with Remote and Mic
"The Apple Earpods, whilst not being revolutionary, offer a good fit and surprisingly good sound for its price. The ergonomics of the iDevice remote included on the cable is excellent, with solid tactile feedback. Soundwise, while these Earpods may not offer any isolation whatsoever, is tastefully tuned for balanced sound. The bass is very good, and midrange frequencies are crisp and clear,..." --AlanChi607
Apple iPod Earphones Headphones for iPod Nano, Touch & Classic
"I'm not sure if this is the same model I got with my iPhone 4s or not. There have been tons of different models of these buds released for different i-devices. The ones that I received with my 4s are (In my opinion ) absolutely worth at least 30-45 $ in my opinion. No they don't sound like my SENN HD 600 by any means at all, but for an earbud they are exceptional. The..." --GoldenGuy66
Signature Acoustics Elements Series C-12
" The C-12s are the first IEM from Indian company, Signature Acoustics. The C-12s use a hand-made wooden housing and a great looking wooden Y-split and slider. At around $60 (AUD) they are a budget IEM which is impressive given their hand-made and all wood design, but the sound is just a little way off from being something to get excited about. Overall, the sound is smooth, laid-back..." --Loquah
Apple In-Ear Headphones with Remote and Mic
" Prelude Apple's stock earbuds that have been shipped with every iPod since its inception are quite possibly the most prolific and widely distributed tech accessory of all time. It's hard to go anywhere without seeing a pair of these things dangling from someone's ears. Casual listeners are bound to be satisfied by the decent performance of the earbuds but what about audiophiles? It's..." --TheGame21x
Apple Earbuds (White with Gray Earbuds)
The iPod In-Ear Headphones have great sound quality and bass response, and come with three different sized caps to ensure the headphones fit comfortably and securely in your ear.
Ipod Earbuds Headphones for Nano
" 6.50 on ebay brand new 9.50 for the pair with a remote for that price. you gotta love it lol " --jockhater2
Apple In-Ear - Headphones (ear-bud)
Great sound quality and bass response / Includes three different sized caps to ensure the earphones fit comfortably and securely / For all iPods
Apple M9128G/A iPod Remote Earphones iPod Remote And Earphones
Listening to your iPod on the go is even more convenient with this wired remote and high-quality earphones set. Compatible with all iPod models except iPod with video, iPod nano and iPod shuffle.


text:
->'''Homer:''' North, South. Ah nuts to this. I'm going to take a short cut.\\
'''Marge:''' Homer, [[ShortCutsMakeLongDelays no, you're going to get lost]].\\
'''Homer:''' Trust me Marge, with today's modern cars you can't get lost. What with all the silicon chips and such.\\
'''Homer:''' Alright, we're here! Let us never speak of the shortcut again.
-->-- ''TheSimpsons'', "Itchy & Scratchyland"
->''"The two boys stood at the front gate for three minutes before facing each other. There was an unsaid vow that stated that neither of them would ever mention this incident ever again."''
-->-- '''''FanFic/YouGotHaruhiRolled''''', on [[ThoseTwoGuys Taniguchi and Kunikida]]'s discovery of Itsuki's SingleTargetSexuality
->''"...I'll say this... The principal offenders were Junpei and Ryoji. We were forced to commit the crime, against our will. We were victims too. ...... ...Now please, do not mention it ever again. "''
-->-- ''Akihiko Sanada, '''{{Persona 3}}''', after getting [[FateWorseThanDeath executed]]''
->'''Rarity:''' (after having her being brainwashed into loving a giant boulder reversed) let us never speak of this again.\\
'''Rarity:''' (When her falling in love with a boulder is mentioned again) I thought we agreed never to speak of that again.
-->-- ''WesternAnimation/MyLittlePonyFriendshipIsMagic'', "The Return of Harmony, Part 2"
->'''"Not another word about this...EVER!!!"''
-->--'''Squidward Tentacles''', ''WesternAnimation/SpongebobSquarepants'', "Squid Baby"
->''"If anybody ever asks, there were fifty of them. And they were...rattlesnakes."''
-->--'''Diego''', ''WesternAnimation/IceAge: The Meltdown''
->'''Skipper''': We take this shame to our graves.
->'''Kowalski''': Agreed.
-->-- ''WesternAnimation/PenguinsOfMadagascar''


text:
It has been a very long time since I have written to you... almost three years since my last substantive post. I stopped writing because I needed time to think. It was in the middle of the 2012 presidential election season and I had grown weary of the melange of meanness, ignorance, and conflict that characterized the campaigns of many of the candidates. But life continued. I have used this time away to live and think and dream more deeply, and am ready to share my thoughts once again.
Lots of very cool things have happened over the last three years. I fell in love, number one, with a chemist from Canada... who grew up on a dairy farm with six brothers and sisters. Our lives as children were different in many ways, yet we certainly share the blessings and lessons of being part of a large family. Larry and I have been able to travel - Spain, Thailand, Mexico, many trips to Canada and Kentucky. We share a love of the outdoors, of learning, and of our recently departed Jon Stewart (sad face).
Daughter Julie got married to Greg in 2014 - I couldn't have asked for a better son-in-law. Son Michael and his wife, lovely Leah, just had baby Nolan Myles a couple of months ago - I am loving watching them become a family. Nolan is already a person! Needless to say, I will be spending more time in New York City and will share photos and experiences.
So what about this sustainable health and wellbeing idea that birthed this blog? Where am I in my understanding of what it means to live as part of a global community, challenged by climate change that affects all of us in so many ways? I have learned so so so much in the last three years - and again, as I have said before, the more I learn, the more I realize that I do not know. I will be writing more about all of this in the near future - the September 2014 People's Climate March, my involvement with Pittsburgh 350.org, what I have learned from my graduate students in the psychology and environment classes. Probably the most important change in my thinking has been the deeper recognition of the sociopolitical, economic, and social justice dimensions of climate change and sustainability issues. Those people with the least contribution to the current climate crisis are suffering the most, and many of the people who hold power have a callous disregard for the well-being of anyone other than themselves. A fine situation, eh?
But for now, I want to focus on the beauty that is all around, in spite of these very hard realities. The love and the new life. And, right now, the breath-taking natural wonders of our world - right now, for me, in British Columbia. We are here for several days, breathing in the clean spruce and cedar scented air, visiting tidal pools that tell secrets about our life's beginnings, climbing mountains and zip-lining back down, eating fresh local food, taking in the beauty of wildflowers.
As we were driving into Port Renfrew, to our destination at Soule Creek Lodge, I felt such gratitude for the lush green all around - the trees, the wildflowers, the grasses. These feed us - they give us the oxygen that we humans and other animals need to live. I felt gratitude, awe, and a sense of protectiveness toward them.
Wild fires are raging along the west coast and mountains of North America, much stronger and fiercer than those in the past. In drought-ridden California, the fires are so bad that extra human power has been needed to fight them - and prison inmates have been tapped to serve (I am sure there is a great story there - will let you know when I know). What we have to lose when we don't protect our natural world is so vast that it almost defies comprehension. I know that I need to know more about how our magnificent and immensely complex environment works, and I also know, understand, that knowledge is not enough. What drives me toward speaking about the climate crisis and its damage to our world is affection - for all of my brothers and sisters - human and other animals, for our resource rich mountains and forests, for our vast life-sustaining rivers and oceans. It is affection for all of these - a wondrous and awesome love - that drives me. We will protect, with our lives, what we love.
In his 2012 Jefferson Lecture to the National Endowment for the Humanities, my man from Kentucky, Wendell Berry, said it best...
"Knowledge without affection leads us astray every time. Affection leads, by way of good work, to authentic hope. The factual knowledge, in which we seem more and more to be placing our trust, leads only to hope of the discovery, endlessly deferrable, of an ultimate fact or smallest particle that at last will explain everything.… It all turns on affection… Don't you see?"


text:
Category archives for Legal
Meaningful gains or huge setbacks?: Congress weighs two bills aimed at reforming the Toxic Substances Control Act
For years, advocates have been calling on policymakers to reform the nation's outdated chemical safety laws. Today, two such bills stand before Congress — one that advocates say better protects the public's health and another that advocates warn is a dangerous step backward.
The trial of former coal company CEO Don Blankenship—the man largely responsible for the Upper Big Branch disaster—is scheduled to begin on April 20. I'm ready to let the Charleston Gazette's Ken Ward Jr. provide the best play-by-play.
Reporters investigate the state of safety at oil refineries following the 2005 Texas City explosion; fast food workers file OSHA complaints; farm workers go on strike in Baja California; and San Francisco officials vote in support of fair working conditions for shuttle bus workers.
In the first study of its kind, researchers have found that improved air quality in southern California had a direct effect on children's respiratory health. The findings point to the effectiveness of smart public health policy — in other words, even as southern California experienced increases in traffic and commerce, aggressive air pollution policies resulted in cleaner air and healthier kids.
OSHA releases new report on injury, inequality and workers' compensation; fast food sues to stop Seattle minimum wage increase; California lawmaker proposes fund for farmworker health; and federal employees can sue for late payments during the government shutdown.
In Boston, you're never too far away from a Dunkin' Donuts. In fact, the Massachusetts-based company inspires a fiery sense of loyalty in many Bostonians. It's kind of hard to give up the city's ubiquitous fast food staple, but Paul Drake is committed.
The public health literature is pretty clear when it comes to income status and poverty and their profound effects on health, disability, disease and life expectancy. But what about income inequality? Does a rising gap in wealth and resource distribution affect people's health too?
King v. Burwell: Supreme Court case could wreak havoc on working families, state governments and health providers
"Established by the state." Those are the four words at the center of an upcoming Supreme Court case that could strip affordable health insurance coverage from millions of working families and result in billions of dollars in uncompensated care costs.
Workers continue to face dangerous exposures to diacetyl; paid sick leave legislation introduced in West Virginia; home health workers rally for living wages; and the rise of the independent contractor classification threatens worker rights.
While silicosis-related deaths have declined, it remains a serious occupational health risk and one that requires continued public health attention, according to recent data from the Centers for Disease Control and Prevention.


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Travelodge Wytheville
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
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- Continental Breakfast
- Business Services
- Wheelchair accessibility
- Free parking
- Pets allowed
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Waterfalls, part 3
... t too dramatic on their own but the backdrops really set them off. Sunburst falls flowed below an old bridge and you can view the falls both above and below the bridge (with a little careful rock climbing). French Broad Cascade flows past and old mill and is joined by another cascade so you get two for one. Unfortunately the mill wheel has been removed and a new bridge has been built over the falls so it has lost a bit of the charm from some of the photos Damien saw while planning ...
Trek Day No.40 - Wytheville, West Virginia
... bigger too. There was also a spot where we could get out and float down on our backs which was really relaxing. Once we arrived back to camp and dried off we had a quick lunch then hit the road. It took us an hour and a half to reach Knoxville, Tennessee which is where Nicole went to Uni so she gave us a quick driving tour round campus. It genuinely looked like the uni's you see in films which was so cool. We then carried onto ...
Into Mayberry
Barbara was feeling much better this morning, so we went into Mount Airy. The locals call it Mayberry, just about as much as they call it Mount Airy. We found our way to Main Street, and the first place we went to was Floyd's City Barber Shop. (His name isn't Floyd, it's Russell. He changed the name of his shop in the 80's from City Barber Shop—a nod to Mayberry, no doubt.) I got my ...
Hiking the Grayson Highands
... but after 2 miles spotted them another mile or more away on a bald knob. We decided not to pursue them, but enjoyed the views along the way. This is a lovely highland area that reminded us much of our home in West Virginia, and Dolly Sods Wilderness area, which is in our own backyard. Still, one never tires of such beauty, wherever it may be found!
After our hike we drove back to Marion, Virginia, and had a late lunch at a barbeque place there.
Meet the hikers
We met up with AJ (our nephew) and Allison, who are hiking the Appalachian Trail. We had breakfast together at the Apple Tree Restaurant in Marion, and they told us all about their adventure on the trail. They are 1/4 of the way through and Virginia has the longest stretch of trail. They took a week off for AJ to collect his PHD diploma, then headed back on the trail. Their packs weigh about 40 pounds, and we watched as they reorganized them with ...


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Reissue of highly collectible promo-only 12" by unlikely Balearic heroes. Released in 1979, this fruitful collaboration between proto-boyband & legendary pop-disco producer Maurice Gibb is slab of chugging syncopated cosmic disco rock.
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Limited reissue of synth masterpiece from 1977. Originally released on small French label Melba, thisrare LP is classic of experimental synth-driven space disco genre. Heavily laden with Korg, Harp & Mini Moogs,ft 5 mesmerising psychedelic odysseys.
The Balearic Sound Of Elkin & Nelson. 4 tracks on Sunkissed Recordings.
LTD EP for the Bombay Disco comp, the story of disco in Hindi films from 1979 to 1985 f/ selections found in the dusty bazaars of India by DJ Brother Cleve. F/ bonus track not found on the comp + a Brother Cleve re-edit of "Udi Baba"
Magical 6 trks from collective minds of Stan Lucas & Leroy Burgess, each responsible for 3 trks. Polished production also lists Patrick Adams on string & horn arrangements & John "Jellybean" Benitez on editing, Noted by some as best disco LP ever.
Strictly limited, hand stamped, individually numbered, no digital. Marbles pink & white vinyl.
Intrusion II, the follow up of their 2013 EP. Another 8 tracks ranging from their own brand of disco to 70's porn funk. Truly an album for a movie that never was.
Reissue 1978 sleazy cosmic disco for 'French' art movie, the kind old boys in rain macs used to duck into soho 'screenings' to watch.
Marcel Vogel's label welcomes disco master & true original, not interested in anything but disco. 2 trks that highlight Glaswegian's need to keep true spirit of disco alive, deftly & tightly edited.
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Boomarm Nation
Concent Productions
Content (L)abel
Deepblak Music
Emphasis Recordings
First Word Records
Fundemental Bass Intelligence
Machining Dreams
Project Mooncircle
Stolen Kisses
Synapsis Records
From the Middle of the Deck
White Stuff


text: As I've mentioned in past columns, the Veterans Health Administration is one of the few clear American success stories in the struggle to contain health care costs. Since it was reformed during the Clinton years, the V.A. has used the fact that it's an integrated system — a system that takes long-term responsibility for its clients' health — to deliver an impressive combination of high-quality care and low costs. It has also taken the lead in the use of information technology, which has both saved money and reduced medical errors.Sure enough, Mr. McCain wants to privatize and, in effect, dismantle the V.A. Naturally, this destructive agenda comes wrapped in the flag: "America's veterans have fought for our freedom," says the McCain Web site. "We should give them freedom to choose to carry their V.A. dollars to a provider that gives them the timely care at high quality and in the best location."That's a recipe for having healthy veterans drop out of the system, undermining its integrated nature and draining away resources.


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"Other than OneNote, this is the most frequently used pen-enabled program I use. I don't know how I would be a professor without it."
Gordon Watts
University of Washington
Seattle, WA
"I have been an avid fan of PDF annotator for six years (I'm in science research). So I just wanted to email you and say THANK YOU! Thanks for creating such a great program and for making software that is so user friendly. As a consumer, I really appreciate it and you are definitely the exception in the field."
Anne Cherry
Harvard University
Cambridge, MA


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Ratings based on 1,332 Verified Reviews
They paid and stayed. We double-checked.
3.8 Room cleanliness
3.7 Service & staff
3.6 Room comfort
3.4 Hotel condition
They paid and stayed.
We double-checked.
We double-checked.
for Everyoneby A verified traveler from Indiana
Great locationPosted Sep 26, 2015
I choose this place for the free breakfast, price and location near the dive shop I was using. Wish they had more for breakfast than cheap processed food in a box.
for Everyoneby A verified traveler from loxahatchee, fl
Very fair pricePosted Sep 7, 2015
Late check in was done at the Holiday Inn. Staff was very nice. I was surprised at how clean the floors were in my room. I usually wouldn't ever walk barefoot but for some reason I did and when I looked at the bottom of my feet they were still clean. Only negatives were there isn't any close parking and finding it is a little annoying.
by A verified traveler from Rockledge FL
Could have been betterPosted Aug 31, 2015
Hotel room door so old and out dated it had to be slammed to close and the locks were mechanical and didn't work very well. Breakfast was good and plentiful. Pool was nice but had a slime coat on the bottom and black mold on the edges.
for Everyoneby A verified traveler
Could be in a better condition.Posted Aug 31, 2015
Cons: It is overpriced.
Despite its prime location and ambiance, the property does not seem to be as well-kept as it was, say, twelve years ago. All in all, though, our room was clean.
for Everyoneby A verified traveler
Decent hotel for scuba diving Key LargoPosted Aug 12, 2015
Decent price, older facility, close to Ocean Divers next door. Basic accommodations, next door parking available when hotel parking fills up. Nice outdoor pool. Some maintenance issues in room and public area but overall decent for the price. Great location close to Dive shop, Divers Direct store, Subway. Will return.
by A verified traveler from naples/zurich
Nice outdated placePosted Aug 9, 2015
Pool dirty, no shade/umbrellas- everthing very old- would not sit on sofa in room...? Got a room upgrade, huge room and kitchen Tv ' s hang nearly at the cealing so high, No door security look from inside The fixer upper would like to work here.
for Everyoneby A verified traveler
Nice view over marinaPosted Jul 31, 2015
Very close to a really nice bar restaurant called skippers and also a more local cheaper one called sharkies. We stayed when it rained like crazy so didn't get into the pool area which looked quite nice. The rooms were a little dated but this reflected in the price I guess, the beds were comfy and reception staff very friendly
for Everyoneby A verified traveler from R
Great location on the marina for fishing and scubaPosted Jul 26, 2015
Pros: Location location location
Cons: Nothing
Location: Central to all Key Largo waterspouts and fishing
Enjoyed our stay, great location central to all in Key Largo especially Sea Dwellers scuba and Ocean Divers. Great dockside restaurant next door with live music, tropical drinks and awesome seafood that will cook what you catch. Two best scuba outfits leave from the dock adjacent to the hotel so no lugging heavy gear all over. Clean room with comfy beds and great AC. Great place we will be back soon.
for Everyoneby A verified traveler
Prisvärt hotellPosted Jul 21, 2015
Bra poolområde. Gångavstånd till 2 bra restauranger. Ungefär 4 km till en badplats. Frukosten är lite spartansk men ändå väldigt bra. Särskilt om man har barn med sig. Rent och snyggt.
for Couplesby Eileen from Solihull
Pleasant dockside hotel in Key LargoPosted Jul 11, 2015 on Hotels
Stayed here for two nights before traveling down to Key West.Hotel is very pleasant and situated along one of the waterways. Free continental breakfast is included and there is seating available on the dockside which makes having breakfast extra enjoyable.


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Quick Links: Integrated Transmission PlanningLocal Area Planning & High Priority StudiesPriority ProjectsBalanced PortfolioPlanning ArchiveDetailed Project Proposals (DPPs)
2015 ITPNT Report2015 ITP10 Report2016 ITPNT Scope2015 ITP10 Scope2014 ITPNT Report2015 SPP Transmission Expansion Plan Report2015 SPP Transmission Expansion Plan Report Project List2013 ITP20 Report2012 ITP10 Report - Board of Directors Approved 01-31-122010 ITP20 ReportITP Manual
Governing DocumentsTariff ViewerITP Schedules
As a FERC-designated Regional Transmission Organization, one of SPP's responsibilities is to create regional transmission expansion plans. With its members, regulators, and stakeholders, SPP creates planning models and studies that determine what new transmission is needed to meet our region's long- and near-term needs and create a cost-effective, flexible, and robust transmission network. SPP does not own or build transmission, though its Open Access Transmission Tariff contains rules that govern transmission planning.
The electric grid is in use every second of every day; the benefits of added transmission are not just long-term, but instantaneous. A well-planned and strong electric grid improves reliability minute-to-minute, optimizes long-term economic benefits, and helps meet public policy goals.


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Notre Dame v Louisville
A Louisville Cardinals cheerleader performs during the game against... Fotografia de notíciasAtuação,Basquetebol,Basquetebol universitário masculino,Chefe da Claque,Desporto,EUA,KFC Yum! Center,Kentucky,Louisville Cardinals,NCAA,Notre Dame Fighting Irish,Partida - Desporto,Universidade de Louisville,Universidade de Notre Dame,VerticalPhotographer Andy LyonsCollection: Getty Images Sport 2013 Getty ImagesLOUISVILLE, KY - MARCH 09: A Louisville Cardinals cheerleader performs during the game against the Notre Dame Fighting Irish at KFC YUM! Center on March 9, 2013 in Louisville, Kentucky. (Photo by Andy Lyons/Getty Images)