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It spreads over a single area of 4,500 square meters around the idea of a shopping street of the city center, where on both sides there are 90 corner shops.
The largest boutique of southern Italy. Born in the early 90s, it is built on three levels with a total area of 5,000 square meters.
With an area of about 10,000 square meters is the evolution of the concept CityModa, offering over 120 corner and many services.
Citymoda with its large boutique is now the absolute reference for all fashion lovers who want to dress with style, trendy, elegant and with a keen eye for quality products.
With stores all over Apulia, in Bari, Modugno (BA) and Lecce and Abruzzo in Santa Teresa di Spoltore (PE) CityModa is one of the leading businesses in the fashion retail of Centre-South of Italy offering clothing, footwear and fashion accessories multibrand.
The project was born in CityModa Modugno in 1995 from an idea of its founder Giancarlo Fiore who understands the changing times in the belief that it could play a small city of fashion with the introduction of innovative services and intercept, in this way, increasingly demanding customer requests.
The concept CityModa is strengthened with the opening of Lecce and Spoltore stores. In fact, the large boutiques with over 5000 square meters of display corners and services such as tailoring, bar, restaurant, game room, spagym, , thermal circuit and hair stylist offers an experience of emotion and gratification.
The flagship model CityModa is the personal shopper that serves as a fashion consultant.
Over 20 years of experience in fashion and always looking towards the new market trends, to anticipate them and live them as a protagonist. These are the main features that have made CityModa one of the main businesses in the South of Italy in the field of fashion retail, which offers clothing, footwear and fashion accessories multibrand and caters to a customer target medium-high.The activities of the group began in 1995 when its founder Giancarlo Fiore after cultivating a long dream, born years ago in a trip to the Big Apple, to create the first fashion citadel of South Italy, decides to start a business project starting with the opening of the first megastore CityModa in Modugno (Ba). Fiore understands that times are changing, customers need to be able to benefit from services (including bar and restaurant), that the concept of sales is rapidly changing and advice on investing, the ability to listen to the needs, purchasing as experience, shop assistant as authentic personal advisor customer CityModa.
Ask for it now, it's easy: the CityCard is absolutely free. Just ask for and fill in the form at CityModa stores.
Regolamento CityModa "Dona il tuo usato" Con il patrocinio morale di Croce Rossa Italiana Caritas Diocesana A.P.S. Equanima Onlus Promozione valida dal 27 marzo 2015 al 30 aprile 2015 sui capi delle nuove collezioni primavera-estate 2015. La promozione è riservata ai clienti Citymoda che porteranno i vecchi abiti usati, in uno degli Store aderenti all'iniziativa: per ogni capo* il cliente riceverà uno sconto del 20% sul capo di nuova collezione acquistato**. Saranno accettati tutti i capi di abbigliamento uomo, donna, bambino ad eccezione di biancheria intima, calzature e accessori non tessili. Lo sconto sarà applicato esclusivamente se il capo verrà consegnato in buono stato di conservazione e lavato. I capi usati verranno devoluti in beneficenza tramite organizzazioni non profit e di...


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Call us now 03330 146 466
Or request a call back
Benefit from the efficiencies of multi-utility.
Secure the advantages of a dual fuel installation.
The full solution from design to connection - we install the gas main, the meter and the outlet pipework.
We offer a complete service from design and quotation through to installation of your mains and services.
Almost every topic covered, from Quotes and Scheduling to Traffic Management and Technical queries.
A range of useful downloads covering everything from Dimensions to Site Preparation Requirements.
Fulcrum, the UK's leading independent energy and multi-utilities service provider, has reaffirmed it… Read more >>
Martin Donnachie has discussed the impressive journey Fulcrum has taken since he became CEO, in Busi… Read more >>
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Phytoplankton Bloom Off Nambia
Photograph - Photograph
A flash of blue and green lit the waters off Namibia in early November 2007 as a phytoplankton bloom grew and faded in the Atlantic Ocean. The Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Aqua satellite captured this photo-like image on November 8, 2007. The bloom stretches from north to south along hundreds of kilometers, though it is brightest in the center of this image. Such blooms are common in the coastal waters off southwest Africa where cold, nutrient-rich currents sweep north from Antarctica and interact with the coastal shelf. At the same time, the easterly trade winds push surface water away from the shore, allowing water from the ocean's floor to rise to the surface, bringing with it iron and other material. The suffusion of nutrients from both the currents and upwelling water creates an environment where tiny surface-dwelling ocean plants (phytoplankton) thrive. Phytoplankton blooms are so abundant off Namibia that their death and decomposition often robs the water of dissolved oxygen. As the plants die, they sink to the ocean floor where bacteria consume them. There is so much plant material that the bacteria use all of the oxygen available in the water before they finish breaking down the plants, creating a dead-zone in the water where fish can't survive. Anaerobic bacteria, which don't require oxygen, take over in the decomposition process, releasing sulfur dioxide as a byproduct. The sulfur dioxide interacts with the ocean water to create solid sulfur and hydrogen sulfide, a poisonous gas, which eventually erupts to the surface, sometimes killing fish. Though no eruption is readily apparent in this image, hydrogen sulfide eruptions are often visible in satellite imagery because the solid sulfur colors the water a milky yellow-green. The bloom shown here persisted over several days. It first became distinct on October 28 (though clouds covered the region on previous days, so it may have developed earlier) and was just beginning to
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Total Performance SportsRead MoreMake yourself a member at Total Performance Sports in Everett, and enjoy working out at a spot you'll love. Whether you're trying to train for a marathon or just want to improve your fitness knowledge, enlist the help of one of the personal trainers on staff. Develop kickboxing skills and discipline during one of these high-intensity kickboxing classes. After a few weeks of enrolling in a cross fit program, you'll be amazed at how much better you feel! Parking is plentiful, so guests can feel free to bring their vehicles.11 Victoria StreetEverett, MA US
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Sadhana Yoga StudiosRead MoreYoga enthusiasts flock to Sadhana Yoga Studios in Everett for all their physical and mental needs. It's certainly time you stopped reading about this studio with its amazing restaurant and finally came in for a bite. With its kid-friendly vibe, this studio is a great spot for families. You may want to pack a change of clothes before heading to Sadhana Yoga Studios, where students rave about the hot yoga classes. Build lean and strong muscles with one of these group kickboxing classes. Parking is plentiful, so guests can feel free to bring their vehicles.326 Chelsea StreetEverett, MA US
Total Performance SportsRead MoreBoost your energy at Total Performance Sports in Everett. Be sure to visit the restaurant at this gym for a delicious meal. Take advantage of the personal trainers here to help you keep your form and get the results you intend. Strengthen your body with the rigorous regimen of cross fit. Patrons will love the number of street and lot parking options close to Total Performance Sports.68 Vine StreetEverett, MA US
Phunk PhenomenonRead MoreFor a great time at the theater, choose Phunk Phenomenon in Everett. While you're enjoying this theater, be sure to check out their amazing restaurant for a tasty meal. This theater is great for families with kids. Parking is plentiful, so guests can feel free to bring their vehicles.1760 Revere Beach ParkwayEverett, MA US


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NFC Times Exclusive – Gemalto's deal to supply TSM technology for Samsung Pay when the mobile-payments service launches in Europe, which Gemalto announced today, might net some favorable coverage for the vendor, but it will do little to help its struggling mobile financial services unit.
NFC Times Exclusive Insight – Retailer consortium MCX didn't fire its CEO, Dekkers Davidson, because one of its major founding merchants, Best Buy, snubbed the group by agreeing to accept Apple Pay. But that was probably the last straw.
NFC Times Exclusive Insight – In his usual gushing tone, Apple CEO Tim Cook proclaimed 2015 as the "year of Apple Pay," though he gave few meaningful details on actual use of the new payments platform since it was launched three months ago or on merchant acceptance or Apple's expansion plans outside of the U.S.
Google is almost certainly in talks with Softcard to acquire the joint venture from its telco owners, but the question is, what would Google gain from such a deal?
UPDATED: NFC Times Exclusive – Ryan Hughes, former chief marketing officer for the Isis joint venture, said he recalls sitting in weekly prioritization meetings on Friday afternoons, and when representatives from the mobile carriers that own Isis would ask why the JV was not making more progress in its rollout, he knew where to point the finger.
LAS VEGAS, Nev. – After Capital One dropped out of the Isis wallet last year, the large U.S. credit card issuer let it be known that one of the biggest reasons it was snubbing Isis was that it believed it had to cede too much control to the third-party wallet provider.
UPDATED: There's little doubt now that Apple will support NFC and mobile payments in its next iPhone. The remaining question is exactly how it will do so.
There is no confirmation yet that Apple will adopt NFC for the forthcoming iPhone 6, but some sources have told NFC Times they believe there is substance to the rumors that Cupertino is finally ready to adopt the technology this year.
NFC Times Exclusive: While banks gearing up for NFC launches are keenly interested in host-card emulation, as this week's NFC Times story about planned NFC launches in France notes, a number of banks in France and elsewhere do not plan to wait until HCE is mature enough for commercial rollouts.
With Google's strong push behind host-card emulation and endorsements from Visa and MasterCard Worldwide, mobile operators, SIM vendors and TSMs are resigned to living with the technology, even publicly supporting it–up to a point.
NFC Times Exclusive Insight – Inside Secure, an early NFC chip supplier, which has since focused on embedded security, has replaced its long-serving CEO Rémy de Tonnac with smart card industry veteran Amedeo D'Angelo, hoping to turn around chronic losses and a plunging share price.
NFC Times Exclusive Insight – Apple has been quietly taking more steps toward a launch of Apple Pay in China, registering a local company in Shanghai and hiring at least two top former Visa China staffers, including the former country manager.
NFC Times Exclusive Insight – With contactless card payment growing more popular in Europe and high-profile NFC mobile-payments services such as Apple Pay starting to gain a foothold there, payment schemes are preparing to significantly raise or eliminate caps on transaction value to try to encourage even more growth in contactless and NFC payments.
NFC Times Exclusive Insight – At least one more of China's big four banks, and perhaps two, have reportedly introduced mobile payments with host card emulation, following Industrial and Commercial Bank of China, which launched HCE-based service in May.
NFC Times Exclusive – A joint venture of Taiwanese banks and banking groups has chosen U.S.-based Mozido to supply it with host card emulation, tokenization and wallet technology, a surprise win for Mozido, which bought the U.S. mobile commerce arm of South Korean IT services firm SK C&C less than a year ago, NFC Times has learned.
NFC Times Exclusive Insight – With SIM-based NFC not taking off in Taiwan and Apple Pay's eventual arrival on the island considered inevitable, a consortium of Taiwanese banks is expediting plans to add host card emulation and tokenization to their conventional TSM offer.
NFC Times Exclusive Insight – U.S.-based unattended retail payments company USA Technologies had nearly 300,000 NFC-enabled cashless payment terminals connected to its network as of the end of the 2015 fiscal year and expects that to grow strongly next year, as mobile-payments services such as Android Pay roll out, the company said.
NFC Times Exclusive Insight – Barclaycard, one of the top payment card issuers in the UK, plans to launch NFC payments from its mobile-banking app in November using host card emulation, which will allow the issuer to avoid putting its payment applications on secure elements to roll out mobile payments.
NFC Times Exclusive Insight – Google officially launched Android Pay in the U.S., its host card emulation-based rival to Apple Pay and its second stab at rolling out NFC payments for in-store payments, following its little-used Google Wallet.
NFC Times Exclusive Insight – MasterCard Worldwide Tuesday announced its hub-like commercial tokenization program, Digital Enablement Express, a widely expected move that matches a similar service that rival Visa introduced in late May, Visa Digital Enablement Program, or VDEP. Like VDEP, MasterCard's new tokenization program has signed up Google and Samsung for their respective payments services, though neither MasterCard nor Visa has announced that Apple would join.
NFC Times Exclusive – Mobile security company Trustonic says its two remaining owners are increasing funding for the joint venture, and it plans to get into new markets, as the company seeks to capitalize on growing interest in securing mobile payment services, among other applications.
NFC Times Exclusive Insight – CaixaBank, Spain's large savings bank and one of the most important early adopters globally among financial institutions of SIM-based NFC payments, has introduced a mobile-payments app based on host card emulation.


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PAWTUCKET – Tolman High coach Craig Giarrusso admits his biggest challenge during preseason practice sessions has been to develop a replacement for four-year starting pitcher (and slugger) Micayla Kirylo.
"In this sport, pitching is obviously the big key," Giarrusso admits. "We have two pitchers we are working with this spring – sophomore Olivia Larson and junior Alex Lacoste. I have a lot of faith in Olivia that she can take over for Micayla and do a good job."
At what point in Tiger Woods's golf career will the media stop trailing the guy around like he's still the No. 1 player in golf?
Even though Woods has been a non-factor in most tournaments since Thanksgiving of 2009, the long-time No. 1 player in the world still gets an inordinate amount of face time on television as he struggles to regain his old form. I guess the guy is news, no matter what he shoots each day. Or maybe we all just like to watch the golfing version of a train wreck unfold each time he plays.


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Capital Markets
Company Overview of MCG Global Partners Fund, L.P.
Company Overview
MCG Global Partners Fund, L.P. specializes in leveraged buyouts and growth capital investments in middle market. The fund initially invests between $10 million to $15 million. It prefers to hold majority interest in its investments.
300 Long Beach Boulevard
Suite 13
Stratford, CT 06615-7116
United States
Key Executives for MCG Global Partners Fund, L.P.
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Our data partners will research the update request and update the information on this page if necessary. Research and follow-up could take several weeks. If you have questions, you can contact them at firstname.lastname@example.com.


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Remember to consider that grain will be no issue whatsoever in this case, due to the lack of magnification. Even in medium format, I think the grain differences are minimal in 11x14 prints.
HP5 is a flatter film in the shadows, and a steeper film in the highlights than FP4. This is my experience, in any case.
I find that HP5 is more versatile, especially in larger formats where grain is not an issue. I say this because the contrast is lower over all. It expands and contracts just fine, if not better than FP4, IMO. However, FP4 does have a different look, with less shadow detail and more highlight detail in a given composition when compared to the same shot on FP4.
I would use both, personally; probably HP5 for silver prints and FP4 for alt processes requiring a high CI.
If I had to pick one, it would be HP5, for the lower contrast and two-speed advantage. These things help with latitude, malleability, depth of field, action stopping capability, and can help minimize your entry into reciprocity failure land. If it is too flat, you can develop it more. It will take it just fine, especially in such a large format.


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BindingGroup Class
Contains a collection of bindings and ValidationRule objects that are used to validate an object.
Assembly: PresentationFramework (in PresentationFramework.dll)
Initializes a new instance of the BindingGroup class.
Gets a collection of BindingExpression objects that contains information for each Binding in the BindingGroup.
Gets whether each source in the binding can discard pending changes and restore the original values.
Gets the DependencyObjectType that wraps the CLR type of this instance. (Inherited from DependencyObject.)
Gets the Dispatcher this DispatcherObject is associated with. (Inherited from DispatcherObject.)
Gets a value that indicates whether the BindingGroup has a failed validation rule.
Gets or sets a value that indicates whether the BindingGroup contains a proposed value that has not been written to the source.
Gets a value that indicates whether this instance is currently sealed (read-only).(Inherited from DependencyObject.)
Gets the sources that are used by the Binding objects in the BindingGroup.
Gets or sets the name that identifies the BindingGroup, which can be used to include and exclude Binding objects in the BindingGroup.
Gets or sets whether the Validation.Error event occurs when the state of a ValidationRule changes.
Gets the object that this BindingGroup is assigned to.
Gets or sets a value that indicates whether the BindingGroup reuses target values that have not been committed to the source.
Gets or sets a value that indicates whether to include the NotifyDataErrorValidationRule.
Gets a collection of ValidationError objects that caused the BindingGroup to be invalid.
Gets a collection of ValidationRule objects that validate the source objects in the BindingGroup.
Begins an edit transaction on the sources in the BindingGroup.
Ends the edit transaction and discards the pending changes.
Determines whether the calling thread has access to this DispatcherObject.(Inherited from DispatcherObject.)
Clears the local value of a property. The property to be cleared is specified by a DependencyProperty identifier. (Inherited from DependencyObject.)
Clears the local value of a read-only property. The property to be cleared is specified by a DependencyPropertyKey. (Inherited from DependencyObject.)
Coerces the value of the specified . This is accomplished by invoking any CoerceValueCallback function specified in property metadata for the dependency property as it exists on the calling DependencyObject.(Inherited from DependencyObject.)GTMT
Runs all the ValidationRule objects and updates the binding sources if all validation rules succeed.
Determines whether a provided DependencyObject is equivalent to the current DependencyObject.(Inherited from DependencyObject.)
Allows an object to try to free resources and perform other cleanup operations before it is reclaimed by garbage collection.(Inherited from Object.)
Gets a hash code for this DependencyObject.(Inherited from DependencyObject.)
Creates a specialized enumerator for determining which dependency properties have locally set values on this DependencyObject. (Inherited from DependencyObject.)
Returns the current effective value of a on this instance of a DependencyObject. (Inherited from DependencyObject.)GTMT
|GetValue(Object, String)
Returns the proposed value for the specified property and item.
Re-evaluates the effective value for the specified dependency property(Inherited from DependencyObject.)
Invoked whenever the effective value of any on this DependencyObject has been updated. The specific dependency property that changed is reported in the event data. (Inherited from DependencyObject.)GTMT
Returns the local value of a , if it exists. (Inherited from DependencyObject.)GTMT
|SetCurrentValue(DependencyProperty, Object)
Sets the value of a without changing its value source. (Inherited from DependencyObject.)GTMT
|SetValue(DependencyProperty, Object)
Sets the local value of a , specified by its dependency property identifier. (Inherited from DependencyObject.)GTMT
|SetValue(DependencyPropertyKey, Object)
Sets the local value of a read-only , specified by the DependencyPropertyKey identifier of the dependency property. (Inherited from DependencyObject.)GTMT
Returns a value that indicates whether serialization processes should serialize the value for the provided .(Inherited from DependencyObject.)GTMT
Returns a string that represents the current object.(Inherited from Object.)
|TryGetValue(Object, String, Object)
Attempts to get the proposed value for the specified property and item.
Runs the converter on the binding and the ValidationRule objects that have the ValidationStep property set to RawProposedValue, ConvertedProposedValue, or UpdatedValue and saves the values of the targets to the source objects if all the validation rules succeed.
Runs the converter on the binding and the ValidationRule objects that have the ValidationStep property set to RawProposedValue or ConvertedProposedValue.
Enforces that the calling thread has access to this DispatcherObject.(Inherited from DispatcherObject.)
A BindingGroup creates a relationship between multiple bindings, which can be validated and updated together. For example, suppose that an application prompts the user to enter an address. The application then populates an object of type Address, which has the properties, Street, City, ZipCode, and Country, with the values that the user provided. The application has a panel that contains four TextBox controls, each of which is data bound to one of the object's properties. You can use a ValidationRule in a BindingGroup to validate the Address object. If the bindings participate in the same BindingGroup, you can ensure that the zip-code is valid for the country of the address.
You set the BindingGroup property on FrameworkElement or FrameworkContentElement. Child elements inherit the BindingGroup from their parent elements, just as with any other inheritable property. A binding on a descendent element is added to a BindingGroup if one of the following situations occurs:
The binding's source and the DataContext of the element that has the BindingGroup are the same object and the BindingGroupName property is not set.
The BindingGroupName property of the binding is equal to the Name of the BindingGroup and they are not explicitly set to null.
In the example of the address, suppose that the DataContext of the Panel is set to the object of type Address. The binding for each TextBox is added to the BindingGroup of the panel.
You add ValidationRule objects to a BindingGroup. The BindingGroup is passed as the first parameter of the Validate method when the ValidationRule runs. You can use the TryGetValue or GetValue(Object, String) method on that BindingGroup to get the proposed values of the object, and the Items property to get the sources of the bindings.
A BindingGroup updates the sources of the binding at the same time instead of each binding being updated separately. When you call one of the methods to validate the data (ValidateWithoutUpdate, UpdateSources, or CommitEdit), the binding for each TextBox in the example is validated and potentially updated. When a binding is part of a BindingGroup, the source of the binding is not updated until you call UpdateSources or CommitEdit on the BindingGroup, unless you explicitly set the UpdateSourceTrigger property.
The following examples create an application that prompts the user to enter the description and price of an item and the date that the offer expires. The application displays the current information for the item below the form. The user can submit or cancel the changes.
The application does the following to achieve this behavior.
Creates a BindingGroup and adds it the root StackPanel when it creates the user interface (UI) of the application.
Calls BeginEdit, CommitEdit, and CancelEdit in the application's logic to enable rolling back changes.
Calls TryGetValue in a Validate method to get the user's input and then check that an item over 100 dollars is available for at least seven days.
The following example creates the user interface (UI) of the application. The root StackPanel has a BindingGroup that contains a ValidationRule that validates the item, as described previously. The binding objects on the Price property and the OfferExpires property become part of the BindingGroup and each binding has a ValidationRule to make sure that price and date, respectively, are valid values. The validation rules for the individual properties run before the ValidationRule on the BindingGroup.
The following example shows the event handlers for the application. When the user clicks the Submit button, the application calls CommitEdit to run each ValidationRule that is associated with the BindingGroup. If each ValidationRule succeeds, CommitEdit saves the values to the object and ends the edit transaction. If CommitEdit is successful, the application begins another edit transaction. When a ValidationRule fails, the Validation.Error event occurs because the application set NotifyOnValidationError to true on the BindingGroup (in the previous example). ItemError handles the Validation.Error event and displays information about the validation error to the user. The example also handles the Loaded event for the StackPanel and the Click event for the Cancel button.
The following example shows the custom ValidationRule ValidateDateAndPrice, which was added to the BindingGroup in the first example. The ValidationRule uses the BindingGroup in its Validate method to get the values the user entered into the form, and checks that if an item is over 100 dollars, it will be available for at least seven days.
Available since 3.0
Any public static (Shared in Visual Basic) members of this type are thread safe. Any instance members are not guaranteed to be thread safe.


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Welcome to WebmasterWorld Guest from 22.214.171.124
Forum Moderators: open
With 4 large sites, all have had little or no activity since turn of the year in terms of spidering - no wonder some of the SERPS are looking a little stale.
Anyone else?
The main pages still rank well, but only about half the site is indexed now.
Index page cache still showing 6 Jan!
Other parts of the site taken in chunks - say a section of the catalogue at a time. Very unusual. Google normally crawls the index every two or three days.
The log file entry:
22.214.171.124 - - [22/Jan/2005:13:07:37 -0500] "GET / HTTP/1.1" 200 3995 "-" "VM4050/132.037 UP.Browser/220.127.116.11.e.1.100 (GUI) MMP/2.0 (compatible; Googlebot/2.1; +http://www.google.com/bot.html)"
The IP belong to Google.
Past thread on this topic:
Gbot Running Hard [webmasterworld.com]
Anyone noticing this?
From de 1400 pages content online, I lost the half of it. Still indexed, but no cache, description, title etc.
I can't wait and sit down to wait till it will be fixed so I removed the "lost" links with the G's URL remover.
All went fine, but since my new removal request for another directory (requested at the 19th, this makes me think that G is really broken), there's still nothing to happen. Normally it will be fixed within 24 hours.
I made some new pages with quality content, with a link from the mainpage. Google did picked it up very quick including titles, descriptions etc. etc.
My personal current conclusion, was that I had a to high KW-density. I'll hang around at 10% density now.
FYI: My site is travel related.


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Top 10 Best Disney Songs
The Top TenXW
Indeed the best song for me. Truly awestruck and stunned by the scene where Elsa sings it and she builds her "ice-lated" castle, it's not just the video I'm impressed in, but above all the song. It is really an inspirational, emotional, and very catchy song. It not only has good beats and instruments, but teaches us to "let it go" let our fears go, don't care what others say, be yourself and have magic, etc. It's also a great song to listen in whatever mood you are. Like I said before, it is an emotional song---it brings out more than one emotion. But for me, what really struck me for this song was the "emotional progress" of it: from despair and isolation, and later on the lyrics, it becomes hopeful, heavenly, and courageous mood to it. I have also listened to the classic and other Disney songs as well, even back before Aladdin and Mulan, and I must say I love them. They're all awesome, including A Whole New World, Circle of Life, When You Wish Upon A Star, Reflection, I'll Make A Man Out of You, and so much more. But for me, above all, the most inspiring and influential song (and probably the best and greatest) is Let It Go. We just can't seem to "let it go".
After the nice digression Disney took with "wreck it ralph", Frozen went back to more traditional ways, has not just one but two Princesses, and a really nice fairytale setting. It also has a fair share of good songs, but none stands out as much as "Let It Go".
There are few songs from musicals wich I would listen to in my freetime, this one definitely is one of them. After all, the movie totally slipped under my radar, until I first listened to this song.
The Lyrics are great to just plain brilliant "It's funny how some distance makes everything seem small"
While the animated sequence is just stunningly beautiful. It just stays in mind, seeing her building this beautiful castle while singing this song.
It fits perfectly to Idina Menzel's voice, while the instrumental part flows just perfecty along. It gives me a shiver down my spine every time when the pace increases after the first refrain.
Its amazing it should be the first
Theirs only one word to describe this song:OVErrated!V82 Comments
The power of this song is incredible. In a very weird way it is extremely catchy and makes you feel epic and masculine when you sing along to this incredible underdog of the Disney song world.
It is fun, it's catchy and it really is a milestone for mulan and the troops. It is great for sing alongs and even in my high school classes people play it and know all the words to it! It also brings a heavy meaning as while shang sings about being a man, the woman impersonating the man manages to raise herself above the stance of pale pathetic woman with no clue. it shows that yes, they did send him daughters when he asked for sons, and she kicks butt! Put that message with a catchy tune, beautiful lyrics and great action behind it, and you can't get a better song!
BEST SONG EVER! I love this movies in general and this song is the biggest reason I do
It is the best song everV53 Comments
I love this song! Me and my friends are always singing it! Thank you so much Disney for making Aladdin! One of the best Disney movies ever to be made! (After Ariel of course - but only because of her hair) ) Anyway, love for Aladdin and Jasmine
The prettiest Disney song in my opinion, I want this played at my wedding LoL
This countdown is rigged! A Whole New World needs to be number one, not that overrated platonic synthesizer Let it Go!
It should have been 1st placeV17 Comments
Colors of the wind is the best disney song ever! It's beautiful how it talks about nature and that its all connected in a cycle that never ends😉 besides its not the typical disney love song 😍 personally my favourite princess is pocahontas she's so real and original (its even based on a real Indian on a real story) she is so different from all the other princesses👸 waiting for prince charming
I love it so much, it actually has meaning. Part of your world and a whole new world is about someone who is desperate to be loved or to love. This should be higher, it's about environment and racist in one some song! Things are more important than to find your true love in one scene then kiss him/her in the next
"How high can the sycamore grow? If you cut it down, then you'll never know" -
This should be the second oneV24 Comments
This song is so heartfelt and beautiful. Jodi Benson's voice is extraordinary! This song was also covered by Chely Wright as it's considered by many to be a "Coming Out" anthem, and is often played at LGBT rights campaigns. If maybe displeased with the entire film, I am almost certain H. C Anderson would have at least loved this song. It captures the feeling of his original fairy tale really well.
I love this song, I sang it all the time when I was little. It drives me crazy when people call it "Part of Your World" though, LoLz
This shod at least be at number 3. Let it go is number 1 for me but part of your world use to be my favorite song. The voice of Ariel (sorry I don't know who is the voice of Ariel) is amazing. This is one of the best Disney songs ever. I like "A whole new world" from Aladdin but I like this better.
This song has strong vocals and needs allot of practice. I wish I could just go and sing this anywhere and sound as beautiful as Ariel. Yet, I could, With LOTS of practice!V15 Comments
As a little girl, I would dream of being able to be Mulan in the movie and sing this song. It has emotion and meaning. Mulan has to deal with the expectations of those around her. Not wanting to upset her family, while still not wanting to be untrue to herself, she's very confused. I'm sure many people can relate to this situation. All in all, a very beautiful song!
And no one does it better than Jackie Evancho on her 2nd PBS Great Performances concert "Music Of The Movies", and on her studio album "Songs From The Silver Screen".
I like this song it is so cool, I sing it the whole time!
"REFLECTION" Mulan!
I really like this song. Every time I hear this song, I feel like I'm in heaven!V19 Comments
This is the best Disney song ever. When I was little I would listen to this song with my mom every day on the way to and back form school. Its simply amazing and it will always be my most favorite Disney song ever. Love it.
This song is so inspiring. I just love Hercules.
I love this song so much! Beyond empowering it really does make you feel like you can go the distance!
I love this song. So great in so many ways. Where is Street Rat on this list? Or the Genie song?V10 Comments
One of the best love songs ever! And from Disney, you know its got to be good. Love the movie too
Jackie Evancho wowed millions around the world with her loveliest of renditions at the Capitol Fourth celebration on July 4 2013..
This is simply the best song out of the lot. My favorite. I love the orchestra, the beautiful lyrics. All of you mention Jackie Evancho, but to be honest, Elton John's version sounds true and beautiful, giving more emotions. Pure beauty.
Jackie's delivery of this piece is stunning... You CAN feel the love - as it pours out of her soul and flows into your own.V12 Comments
I can't believe this is rated so low, it should at least be in the top 10. This song is amazing! I love the magic it shows and how much it reminds me of being a kid. This song is definitely one of my disney favorites, I love Lion King so this isn't my single favorite but it's definitely one of them.
This should be number one. It's the most powerful and is beautifully written, The part when the orchestra gets going again, followed by lyrics, and the animals bellow and bow... Chills!
Absolute best song ever created in the history of the world... Not only should it be #1 on the disney best songs list, but it should be #1 on any best songs ever list
This, friends, is probably one of the most phenomenal Disney songs ever made. It's just so powerful, which is a quality many other Disney songs have, but it speaks to me. This should be AT LEAST in the top 5!V16 Comments
Listen to the version by Jackie Evancho. Disney should be playing it in the entrance to Fantasyland at all of the parks.
Classic Disney song from a classic Disney movie! Beautiful lyrics, very inspirational... Should be number one. When I hear "Disney", I tie it back to this amazing song.
This song never grows old. Jackie sang this exquisitely during the pope's visit to Philadelphia in 2015. See the video on YouTube. - BobG
It is one of the classics and is so deep in its lyrics plus it's sung by Jimminy Cricket. Just for that it should be in the top 5V10 Comments
The Newcomers
Maleficent convinces mal that the only way to live life as a queen is to be evil.
The Contenders
Last is the classic "Under the Sea" sung by Sebastian. This song is awesome, not much left to say
This song has a fast beat and you need to be fast to sing it, too. You need practice. However, depending on what kind of singer you are, I don't recommend this song for a talent show.
Lovvvvvee itt! So catchy -
Not my favourite Disney song, but it is still one of the best classical Disney songs of all time. I would choose this over let it go from Frozen any day.V10 Comments
This song has such special meaning to me. Not to mention how great Phill Collins voice is! It is definitely in my top 10.
I am simply in love with this song. I like the song better than the movie.
It is such a nice song and so fun to sing along to!
This song is just so touching and heartwarming. Should be in the top tenV9 Comments
As a general rule, I LOVE all Disney songs, but this one will always stand out to me. First of all, the scene it plays in is just beautiful. It's just Flynn and Rapunzel on a romantic boat ride at night, with thousands of lanterns covering the sky. It's just gorgeous. Mandy Moore's voice is so sweet and lovely, and it goes very well with Flynn's voice. The melody is soft and romantic, the lyrics are absolutely adorable, and this song just gives me goosebumps every single time.
This is actually my favorite song! Like you said beautiful melody and lyrics, Mandy Moore and Zachary Levi had excellent singing voices for this song! They matched well together, I was disappointed when this song lost to Toy Story 3's We Belong Together, Toy Story's had enough glory this song was so much better! The animation sequence with the lanterns was absolutely breath-taking!
All these songs are great but this one's so cute. And I know you can't beat the classics but for a recent Disney film, this one is one of my favourites :)
Top 10 it is amazing!V17 Comments
"Yes, our teeth and ambitions are bad. Be prepared! *Scar's evil laugh*"
Who needs a king No king No king
Just the best villain song! Really funny and dark but also powerful
Anybody who does not like Scar is evil.V6 Comments
I LOVE THIS SONG Please vote lion king I LOVE IT! I also like beauty and the beast! Hope you like them oth as much as me! P. S DISNEY IS BRILLIANT KEEP WATCHING DISNEY! P.P. S I hope Disney make more movies YEAH!
how can you not smile when you hear this?
You just can't beat it when ever it comes on I just have to smile and sing along!
This is the best song from a Disney movie.V7 Comments
The way Megara from Hercules is feeling like she loves him but doesn't want to admit it is priceless.
I love this song so much! Its one of my favorite Disney love songs! It should be higher! - ortolani
It's a great song I have it stuck in my head "my head is screaming get a grip girl! " Is my FAVORITE lyric
This is my second favorite Disney song!V7 Comments
It means no worries for the rest of your days
I absolutely love Let it go right now. But this song is my childhood! It ill always be one of my favorites! I am quite surprised it's not in the top 10!
Forget yolo! Hakuna Matata is the motto! Love this song!
It's our problem free, philosophyV8 Comments
this is needed in list... oscar winning track.. one the most magical songs from disney.. add this to list... and watch... how many loved it.. thanks - hani_420
Tied for my favorite Disney song ever written, not only because the melody is stunning, but because the scene that accompanies this song is so marvelous. Simply astounding.
The only song that could possibly beeat it is God Help the Outcasts.
I thought this song was called "Tale As Old As Time"... either way, it's a great song. Really beautiful, and also moving.
Best disney song everV7 Comments
I love this song why isn't higher on the list
Am I the only one that thinks this song is the best ever? Anybody? No? Fml...
Clopin's voice actor did an amazing job on this song. I would pay someone a million dollars if they could hit that high note at the end. Move this up people!
God, if I ever met someone with this man's voice, I would be so utterly awestruck. The note at the very end hits me the hardest and is impossible to replicate. Thank God this wasn't buried at the very bottom.
So powerful and spiritual.
Better than that Frozen crap.V5 Comments
Related ListsBest Disney Channel Songs Best Disney Villain Songs Best Disney Channel Show Theme Songs Worst Disney Animated Film Songs Top 10 Saddest Disney Movie Songs
List StatsUpdated 8 Oct 2015
131 listings
5 years, 251 days old
Top Remixes (47)
2. Part of Your World - The Little Mermaid
3. Go the Distance - Hercules
2. I Won't Say (I'm In Love) - Hercules
3. When Will My Life Begin - Tangled
2. Go the Distance - Hercules
3. I See the Light - Tangled
View All 47


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Festival Bars
The Festival Bars give Festival goers the chance to sample some of the South West's finest drinks.
The Festival Bar in Exeter Castle Courtyard in association with The Well House Tavern opened throughout the Festival including the Festival Party After Dark nights. A great selection of craft beers, real ales and ciders from many local producers including Otter Brewery, Sheppy's Cider, Green Valley Cyder, Clearwater Cask Ales, Branscombe Vale Brewery, Hunters Brewery, Killerton Cider, Barum Brewery and Exe Valley Brewery were available for festival goers to enjoy!
The Festival Gardens Bar in Northernhay Gardens, offered relaxing surroundings and is run by the Exeter Brewery who in addition to their own great range of real ales ran a full bar, including Gin Fizz, fine local wines and ciders plus some guest ales.
Please drink responsibly. www.drinkaware.co.uk
Latest news
Camper Coffee Co awarded 'best stand' at The Exeter Festival of South West Food and Drink 2015
14 May 2015Read article here
THOUSANDS TURN OUT FOR THE EXETER FESTIVAL OF SOUTH WEST FOOD and DRINK 2015
14 May 2015Read article here
Exmouth Bakers triumph in the Exeter Food Festival's Westcountry Bakery with Glenn Cosby
30 April 2015Read article here


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|Reviews for Heart of Thunder
| redbudrose chapter 1 . 12/26/2014
This was great one shot.
| maevelin chapter 1 . 3/2/2014
Dark story but an enjoyment to read!
| Terrible chapter 1 . 9/1/2013
| ThinkTwice123 chapter 1 . 7/8/2013
love it! love dark klaus and caroline still loving him and staying by him despite everything that he does.
| tasna chapter 1 . 4/2/2013
dude, you made me cry with joy and sweet love and passion at the same time... You rock,
| HelloCutePanda chapter 1 . 1/6/2013
Great story - very raw and real. I can see Klaus never being good. It just isn't in him. And Caroline - it is sad to see her like this. But that is what so good about this story - it makes you care.
| LoveOblivious chapter 1 . 9/30/2012
This was, just, equal parts of beautiful and heartbreaking. You really captured that 'semi-abusive' relationship. And it's definitely how I would see a dark!Klaus acting.
| Jeremy Shane chapter 1 . 9/6/2012
Good Story
| KlarolinexDelenaxLove chapter 1 . 9/4/2012
Oh how I love reading your stories, especially when they're about Klaus and Caroline . Such an amazing author or authors:)
| lateVMlover chapter 1 . 9/2/2012
You're a powerful writer.
| Matt-On-Matt chapter 1 . 8/27/2012
It made me sad, it would have been redeemable had he said he loved her as well. Please write something happier next time
| witchdrugs chapter 1 . 8/25/2012
I saw people talking about this one on your tumblr and decided to check it out... Well, love it! Klaus isn't this dark on the show, but maybe he should be? I don't know, he was supposed to be the baddest vampire ever.
Anyway, I can totally see it. Really good, really well-written :3
| Guest chapter 1 . 8/25/2012
hmmm although I DO NOT approve of this kind of things in real life (NO FUCKING WAY), this IS fiction and they're not exactly human anway. And the writing is really good too. :)
| moon2012 chapter 1 . 8/24/2012
Klaus is dark and evil...but holding out hope that love can bring out a diff side even for the most evil person..nicely written though :)
| KindOfAmy chapter 1 . 8/24/2012
This literally made my eyes water, and not just because of it being Klaroline but because it had to do with an experience I had personally. I can't even tell you how beautiful this is. I really don't have the words. I wanted to tell you this not just because you are an amazing writer, but because you affected me as a human being. Your words touched me. They soothe my aching soul. Thank you so much for this.


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Blue Moutain Bassmasters will be holding an OPEN BUDDY Tournament on Sunday, OCTOBER 4TH at Lake Nockamixon. Tournament application can be found here.
Pyatt Wins Again – Elk Neck!
(5/31/2015 Susquehanna Flats – Elk Neck, MD)
The 3rd tournament of the year brought the club to the Susquehanna Flats @ Elk Neck. This is the club's first time at Elk Neck in quite some time.
Glenn Pyatt would continue his winning ways with a 4 fish 12.91 lb. bag. This is Glenn's 2nd win in a row. Glenn's bag edged Joe Dziedzic 10.61 lbs. and Jess Salmon 10.44 lbs.
Joe Dziedzic would win lunker with a 4.4 lb largemouth.
The club recorded 16 bass for a total weight of 43.18 lbs (2.70 lb avg).
Next Stop: Swartswood Lake, NJ (June 28th)
Lehigh Valley Junior Bassmasters – Susquehanna Flats Tournament
High School Anglers Preparing For Their SAF Quad-State Tournament
Student anglers from seven different school districts in the Lehigh Valley and beyond consisting of: Nazareth, Parkland, Easton, Emmaus, Northern Lehigh, Central Bucks and Philadelphia, make up the LV Junior Bassmasters Club. This is the second year for the LV Junior Bassmaster's Club which has increased from four anglers last year to twenty-four this year.
These student anglers have been fishing local club tournaments with established bass clubs, when there are openings, and their first qualifying tournament was this past weekend down at Elk Neck State Park in Maryland. The anglers had a great time and the sixteen student anglers caught a total of eight fish for a total weight of 21.14 lbs. While fishing this weekend anglers learned a lot about fishing from their boat captains and a great day was had by all. Their next qualifying tournament will be on June 14th at Lake Wallenpaupack and their SAF Quad-State Championship, which will consist of teams from Pennsylvania, Delaware, New Jersey and Maryland, will be at Elk Neck State Park on July 25th.
Team fishing allows the student anglers to fish as a team with a boat captain/owner and the boat captain navigates the boat to where the anglers would like to fish. Each team of anglers can weigh in five bass, smallmouth and largemouth. People willing to help or for more information can go online to www.pabass.com to get in touch with PA Bass Federation Vice President, Mike Sabbi or Youth Director, Greg Smith.
Tournament Results can be found here.
Pics From The Day
Pyatt With Win At Hopatcong!
(5/3/2015 Lake Hopatcong, NJ)
On May 3rd, the club traveled to Lake Hopatcong for our 2nd tournament of the year. After a club blank at Merrill Creek, everyone was eager to get on some fish.
On this 2nd tournament, Glenn Pyatt would take top honors with a 5 fish 11.5 bag. Glenn's limit would be the only limit of the day. Rounding out the top three: (2) Lee Buskirk – 6.26 lbs (3) Joe Dziedzic 4.88 lbs.
Tournament lunker went to Lee Buskirk with a 3.44 lb lunker.
Overall, the club recorded 21 bass for a total weight of 37.16 lbs (1.77 lb avg).
Next Stop: Susquehanna Flats – Elk Neck (May 31st)
Bust on Merrill Creek!
(4/19/2015Merrill Creek, NJ)
On April 19th, we kicked off the 2015 Tournament Trail. With a late ice out, the water temp was a chilly 39 degrees.
Mother Nature would prove the winner on this day but keeping anyone from catching a keeper. For the first time in club history, no keeper bass were recorded!
Focus now turns to Lake Hopatcong on May 3rd.
Jess With Victory at Wallenpaupack!
On Oct. 12th, we held our final tournament of the year at Lake Wallenpaupack, PA.
Jess Salmon earned the victory with a 4 fish 6.51 lb limit. Rounding out the top 3 were: (2) Dave Birchler 5.7 lbs and (3) John Detweiler 5.33 lbs.
Jess also took tournament lunker with a 3.01 lb bass.
Overall, the club recorded 17 bass for a total weight of 30.58 lbs (1.80 lb avg).
Hughes Takes Top Honors At Oneida!
On Sept. 28th, we held our 8th tournament of the year at Lake Oneida, NY. This was a one day tournament. Mother nature provided for an interesting day by blanketing the area in thick fog for most of the day.
Mike Hughes earned his first Blue Mountain Bassmasters victory with a 5 fish 15.63 lb limit. Mike's bag was anchored by a 3.58 lb lunker which would prove to be tournament lunker. Rounding out the top 3 were: (2) Joe Dziedzic 14.35 lbs and (3) John Welsh 13.61 lbs.
Mike's lunker of 3.58 lbs narrowly edged three other lunkers that were within 0.05 of the top weight.
Overall, the club recorded 45 bass for a total weight of 117.75 lbs (2.62 lb avg).
Dziedzic Earns Another Victory on Susquehanna Flats!!
(9/14/2014-Northeast River, Susquehanna Flats, MD)
Our 7th tournament of 2014 was held on September 14 on the Susquehanna Flats – Northeast River. This day proved to be very difficult with only 6 bass being recorded.
Joe Dziedzic earned his 2nd victory in a row with 1 fish weighing 3.98 lbs. Joe edged out Rich Heiserman's 3.82lbs. John Detweiler was 3rd with 2.26 lbs.
Joe also earned lunker with his 3.98 lb largemouth.
Overall, the club recorded 6 bass for a total weight of 13.57 lbs (2.26lb avg).
Dziedzic Earns Victory at Blue Marsh!!
The 6th tournament of 2014 was held on August 17th at Blue Marsh Lake. Mother nature gave us an alternating pattern of sun and drizzle for most of the morning.
Joe Dziedzic earned victory with a 4 fish 10.14 lb bag. Joe's bag was anchored by a 3.23 lb lunker. Rounding out the top 3 were: (2) Keith Paff 9.55 lbs and (3) John Welsh 9.29 lbs.
Keith Paff also earned lunker of the tournament with a 3.71 lb lunker.
Overall, the club recorded 24 bass for a total weight of 54.87 lbs (2.29 lb avg).
Norms Wins At Cayuga – 2nd of 2014!!
The 5th tournament of the 2014 Tournament trail took the club to beautiful Cayuga Lake, NY. On this tournament, we would be sharing the lake with some Elite Anglers getting in some pre-tournament practice.
For this 2 day tournament, Norm Post recorded his 2nd win of the year with a 10 fish 21.86 lb limit. Norm's 2 day bag was anchored by a 4.1 lb largemouth. Rounding out the top 3 were: (2) Glenn Pyatt 20.8 lbs and (3) Dave Birchler 19.78 lbs.
Rob Fischl would take tournament lunker for 2nd tournament in a row with a 5.04 lb lunker.
Overall, the club weighed 46 bass for a total weight of 101.65 lbs (2.21 lb avg).
After 5 tournaments, Norm Post has a commanding lead in the 2014 Tournament Trail with 108 points. Joe Dziedzic and Glenn Pyatt round out the top 3 for the year.


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New York Dinner Cruise with Buffet
Special Offer
- 3-Hour Cruise on the Hudson and East River
- Freshly prepared buffet dinner
- DJ entertainment and dancing
- Stroll under the stars and watch the skyline of Manhattan pass by you
Why Our Insiders Chose This Tour
If you want the fantastic views of a dinner cruise coupled with a lively, casual atmosphere, this fits the bill. A generous buffet with great music and dancing make for a night to remember.
Recent Photos of This Tour
New York 107Photo by: Trudi S
03 2012
Outter deck dinner cruise NYPhoto by: Debra M
09 2015
barcoPhoto by: bruno
08 2015
Pista de dança após ...Photo by: RAPHAEL S
12 2014
Enjoying the deckPhoto by: Lauren F
11 2014
Before boarding the boatPhoto by: Lauren F
11 2014
A grand welcomePhoto by: Valerie E
04 2014
Spirit Cruise shipsPhoto by: Valerie E
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Soiée Diner ...Photo by: Malik S
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11 2013
Statue of Liberty taken from ...Photo by: Linda K B
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What You Can Expect
Everything is freshly prepared in our shipboard galley and served in the true manner of an ocean-going cruise buffet. Savor your culinary selections over perfectly-paced dinner music and a never ending view of Manhattan's breathtaking skyline through the panoramic windows.
While you relax on the vibrant decks, enjoy your meal and take in the endless stream of city sights. Later, head topside and relax on the outdoor patio deck for views of Manhattan's magnificent skyline.
Hit the dance floor as our hottest DJs spin your favorite tunes and let you rock 'til we dock!
- Organic Mixed Field Greens
- Organic Spinach and Kale Salad
- Mediterranean Couscous and Chickpea Salad
- Roast Salmon and Tilapia
- Herb Roasted Chicken
- Twice Cooked Beef Short Ribs
- Baked Ziti
- Roast Broccoli
- Mashed Potatoes
- Mini Pastries
- Cookies
- Brownies
- Seasonal Fruit
Customer Reviews
October 2015
Great experience, romantic package recommended, wonderful views of Manhattan at night.
October 2015
it was absolutely fantastic! Food was really very good and super fresh, staff very nice, and amazing view. I would recommend this cruise to anyone and will probably go again myself.
September 2015
Just the bomb. This was a great dinner cruise. The food was tasty. The music was grand, and the service was the best. We had a ball. We will book this cruise again while visiting New York.
September 2015
Location was easy to find and the night lights were everything we expected. Enjoyed Mike, a great guy from Canada. Food was plentiful and good and the entertainment---other tourists dancing to disc jock --was a little something else.
September 2015
excellent trip food was excellent and great customer service
September 2015
Well worth the money. very helpful lovely staff from booking to on the boat I will book again with this company thanks for such a nice trip.
September 2015
Very good cruise. Wonderful view of manhattan at dusk and at night. Nice music and good food.
August 2015
Awesome...always the best choice to celebrate events with friends and relatives . Everything was perfect!
August 2015
Excellent! I figured the food would be mediocre at best, but it was excellent. It was better than most catered events including weddings. It seemed fresh and the chicken and salmon were moist and cooked perfectly. We were very lucky and had a smooth ride because of calm waters. The staff were very friendly and attentive. I would highly recommend Spirit Lines for an evening dinner cruise to anyone visiting Manhattan. The sky line of NYC and NJ is spectacular at night and Lady Liberty never looked so beautiful! I will definitely do it again, but only on the Spirit line.
August 2015
It was amazing. The crew was very respectable to the public. The captain was very personable. I would recommend this to many!
Special Offer
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Women should take steps to boost their self-esteem before resorting to cosmetic surgery, a psychologist has claimed.
According to Ingrid Collins, a consultant psychologist at the London Medical Centre, women are hugely influenced by media images and many seek cosmetic surgery because of misconceptions about the way they see themselves, regardless of how they actually look.
"We look at the airbrushed images of supermodels and we see that as the norm," Ms Collins explained.
The expert said that patients should be encouraged to address their self-esteem and become happy with their own body.
"Their wish to opt for surgery is not necessarily a lot to do with being too big, too small, too thin or too fat, but a lot more to do with how they see themselves.
"That is what needs to be focused upon first," she claimed.
Apart from counselling, patients are also advised to try other methods such as hypnotherapy or herbal treatments before opting for cosmetic surgery.


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Aeran AutotechGurgaon, Haryana(Verified Supplier)
Call Us: +91-8049592816
Products & Services
Company Profile
We are one of the leading manufacturers and suppliers of a wide range of Auto Components, Bracket Alternater and Job Work Services. These components are widely valued for their superior quality, durability and anti-corrosive nature.
Fact Sheet
|Nature of Business
|Number of Employees
11 to 25 People
Contact UsAeran AutotechDinesh AgarwalCall Us: +91-8049592816
Plot No. 222, Sector 7, IMT Manesar
Gurgaon - 122050
Haryana, India
Gurgaon - 122050
Haryana, India
Send your enquiry to this supplier


text:
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Saturday Karaoke Night on Clematis
Everyone wants to be a rock star. The thing is, you kind of need a stage presence to make that possible. They say practice makes perfect: Here's your chance to jam out in front of a live crowd at Saturday Karaoke Night on Clematis at http://www.voiceplaces.com/broward-palm-beach/palm-sugar-22353726-l. It takes place every Saturday night from midnight to 3 a.m. There is no cost to attend. Food and drinks are available for purchase.
Saturdays, 12-3 a.m. Starts: Feb. 1. Continues through March 29, 2014
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Sign up for our weekly guide to events in South Florida, and never be bored again. With suggestions for every day of the week, our recommendations will keep you busy on any budget.
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Rachael Bletchly finds that being a golf widow at a Spanish sports resort is quite tolerable indeed
The NEC in Birmingham hosts the event from October 13 to 18 as UK caravanning and motorhome breaks are booming
Until now, Saga has operated only second-hand vessels but the sleek new ships will have 540 suites, each with its own balcony
Prefer screech breaks to beach breaks? Head to Universal Studios in Orlando for a Halloween fear fest
Break into winter in style this season with a sunny escape to one of these glamorous hotspots, without breaking the bank
What's your high score?
The Mirror


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Page 1
Molecular Cell
Phosphorylation-Dependent Regulation of PSF
by GSK3 Controls CD45 Alternative Splicing
Florian Heyd1and Kristen W. Lynch1,*
1Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 422 Curie Boulevard, Philadelphia, PA
*Correspondence: firstname.lastname@example.com
DOI 10.1016/j.molcel.2010.09.013
Signal-induced alternative splicing of the CD45 gene
in human T cells is essential for proper immune func-
tion. Skipping of the CD45 variable exons is
controlled, in large part, by the recruitment of PSF
to the pre-mRNA substrate upon T cell activation;
however, the signaling cascade leading to exon
exclusion has remained elusive. Here we demon-
strate that in resting T cells PSF is directly phosphor-
ylated by GSK3, thus promoting interaction of PSF
with TRAP150, which prevents PSF from binding
CD45 pre-mRNA. Upon T cell activation, reduced
releasing PSF from TRAP150 and allowing it to bind
CD45 splicing regulatory elements and repress
exon inclusion. Our data place two players, GSK3
and TRAP150, in the complex network that regulates
CD45 alternative splicing and demonstrate a para-
digm for signal transduction from the cell surface to
the RNA processing machinery through the multi-
functional protein PSF.
Signal-induced alternative splicing is a primary, but poorly
understood, mechanism for regulating protein isoform expres-
sion in response to changing cellular environments (Lynch,
2007; Shin and Manley, 2004). In humans, examples of signal-
induced splicing regulation are known to play essential roles in
diverse cellular responses including neuronal depolarization,
insulin signaling, and T cell activation (An and Grabowski,
2007; Chalfant et al., 1995; Lee et al., 2007; Lynch, 2007).
However, surprisingly few cases have been studied in detail
(Shin and Manley, 2004; Lynch, 2007). Therefore, despite the
functional importance of linking extracellular stimuli to pre-
mRNA processing, the mechanisms governing this regulation
are mostly unknown.
A well-documented example of signal-induced splicing regu-
lation is the transmembrane tyrosine phosphatase CD45, which
encodes at least five isoforms as a result of tightly controlled
alternative splicing (Figure 1A; Hermiston et al., 2002). CD45 is
expressed on all nucleated hematopoietic cells and has regula-
tory functions in a variety of signal transduction pathways,
including cytokine-, interferon-, and antigen receptor-mediated
signaling (Hermiston et al., 2002). In T cells, where CD45 plays
an essential role in signal transmission from the T cell receptor
ping of three exons, thereby increasing expression of the small-
est CD45 isoform, CD45R0 (Figure 1A; Hermiston et al., 2002).
This differential CD45 isoform expression in naive versus
activated and memory T cells has long been used as the defining
marker of these T cell states. At a functional level, the activation-
induced exon exclusion in CD45 has been suggested to play an
important role in the homeostasis of the immune system as the
resulting CD45R0 protein forms catalytically inactive dimers
that attenuate T cell signaling (Hermiston et al., 2002; Xu and
Weiss, 2002). Consistently, a silent point mutation in CD45
exon 4 that disrupts the essential splicing regulatory element
ESS1 (exonic splicing silencer 1; Figure 1A) results in aberrant
exon inclusion, loss of CD45R0 isoform expression, and
increased susceptibility to several autoimmune diseases in
humans (Jacobsen et al., 2002; Lynch, 2004).
In recent work, we and others have demonstrated that PSF
and hnRNP L-like (hnRNP LL) bind to the ESS1 regulatory
element in stimulated cells and mediate the increased skipping
of the CD45 variable exons observed upon T cell activation
(Melton et al., 2007; Oberdoerffer et al., 2008; Topp et al.,
2008; Wu et al., 2008; Motta-Mena et al., 2010). Both PSF and
hnRNP LL are RNA-binding proteins that have been shown to
regulate the alternative splicing of many genes in addition to
CD45 (Hung et al., 2008; Oberdoerffer et al., 2008; Shav-Tal
and Zipori, 2002). Moreover, PSF is a highly abundant nuclear
protein that has functions in a range of RNA biogenesis
processes from basic splicing catalysis to transcription to
nuclear export (Shav-Tal and Zipori, 2002). However, how the
various activities of PSF are regulated in cells is not yet clear.
In terms of CD45 alternative splicing, the stimulation-specific
activity of PSF and hnRNP LL is due to the fact that these
latory sequence in activated versus resting T cells (Melton et al.,
2007; Motta-Mena et al., 2010; Topp et al., 2008). In the case of
hnRNP LL, this activation-induced binding is readily attributed to
an increase in protein expression in activated T cells (Topp et al.,
2008). By contrast, nuclear PSF expression remains unchanged
upon activation (Melton et al., 2007), suggesting that its binding
to the CD45 pre-mRNA is regulated at a posttranslational level.
However, the underlying signaling cascade regulating PSF
function has thus far not been characterized.
126 Molecular Cell 40, 126–137, October 8, 2010 ª2010 Elsevier Inc.
Page 2
Glycogen synthase kinase 3 (GSK3) was initially described as
an enzyme regulating glucose metabolism but has since
attracted much interested due to its involvement in many other
cellular processes (Cohen and Frame, 2001). For example,
GSK3 has been linked to the innate immune system (Martin
et al., 2005) and is involved in regulating neuronal cell fate
and development of tauopathies (Plattner et al., 2006). In the
acquired immune system, specifically T cells, GSK3 activity is
decreased upon antigen stimulation through phosphorylation
on serine 9 (Ohteki et al., 2000; Welsh et al., 1996). The
reduced GSK3 activity has been shown to be involved in medi-
ating the CD28 costimulatory signal and is thus required for an
optimal T cell response (Diehn et al., 2002). In our present
work, we have identified an additional role of GSK3, the modu-
lation of alternative splicing. We show that in resting T cells,
GSK3 directly phosphorylates the splicing regulatory protein
PSF. In this phosphorylated form, PSF is sequestered in
a complex with TRAP150, precluding it from binding the
ESS1 sequence in CD45 alternatively spliced exons. Upon
T cell stimulation, reduced GSK3 activity leads to reduced
PSF phosphorylation, thereby releasing PSF from TRAP150
and allowing it to participate in activation-induced CD45 exon
skipping. Thus, we have now identified the complete signaling
cascade linking T cell receptor engagement with PSF-mediated
exclusion of alternatively spliced CD45 exons and have impli-
cated GSK3 and TRAP150 as two critical regulators of this
PSF T687 Is Differentially Phosphorylated in Resting
versus Activated T Cells
Previously we have demonstrated that PSF is uniquely recruited
to the ESS1 regulatory element of CD45 variable exons in
response to cell stimulation, even though this protein is present
at equal concentration in the nuclei of resting and activated cells
(Melton et al., 2007; Motta-Mena et al., 2010). Importantly, PSF
purified from activated cells has silencing activity on the CD45
variable exons in in vitro splicing assays, while PSF purified
from resting cells does not (Melton et al., 2007). Moreover, using
the MS2 system to tether PSF to a model exon, we find that
forced recruitment of PSF results in exon exclusion under both
resting and activated conditions (data not shown), suggesting
that the activity of PSF is primarily regulated at the level of
RNA binding. Taken together, these data led us to test the
hypothesis that PSF itself is differentially modified in resting
versus activated T cells in a manner that controls its ability to
bind to CD45 exons and cause repression.
237 33
Figure 1. Phosphorylation of PSF on T687 Is Decreased upon T Cell Stimulation
(A) Schematic of CD45 showing expressed isoforms, ESS1 silencer sequence, and core motif common to ESS1 and other variable exons through which PSF
(B) Endogenous PSF immunoprecipitated from nuclear extract from either untreated or PMA-stimulated JSL1 cells (72 hr), or IP with unrelated control antibody,
western blotted for total PSF or with antiphosphothreonine (P-Thr) or antiphosphotyrosine (P-Tyr) antibody. Size of PSF in anti-P blots is as indicated.
(C) JSL1 cells stably expressing indicated Flag-tagged PSF variants were lysed, subjected to anti-Flag immunoprecipitation, and blotted for P-Thr or Flag.
Nonspecific band is labeled with an asterisk.
Molecular Cell
TRAP150 Binds to PSF to Limit Splicing Activity
Molecular Cell 40, 126–137, October 8, 2010 ª2010 Elsevier Inc. 127
Page 3
We first compared possible posttranslational modifications of
PSF precipitated from resting versus activated JSL1 cells that
we and others have demonstrated to be a faithful model for
antigen-induced alternative splicing of CD45 when stimulated
with the phorbol ester PMA (Lynch and Weiss, 2000;
Oberdoerffer et al., 2008). Using an antibody specific for phos-
phothreonine, we observed a substantial decrease in threonine
phosphorylation under activated conditions (Figure 1B and see
below). In contrast, global tyrosine phosphorylation of PSF is
weak and does not change in the presence of PMA
(Figure 1B). A database search (http://www.phosphosite.org/)
revealed only one site of predicted threonine phosphorylation,
namely T687. Consistent with this prediction, either deletion of
antiphosphothreonine reactive band (Figure 1C). This result is
further supported by a previous study which mapped threonine
phosphorylation in PSF to the C-terminal third of the protein
(Shav-Tal et al., 2001). Therefore, while we cannot entirely rule
out the presence of additional phosphorylated threonine
residues which may escape the detection with the antibody,
we conclude that T687 is the primary site of threonine phosphor-
ylation in PSF.
PSF Specifically Associates with TRAP150/BTF in
a Phosphorylation-Dependent Manner
Strikingly, the immunoprecipitations of PSF derivatives also
revealed a protein of around 135 kD that coprecipitated with
the wild-type PSF and the T687D mutant but was largely absent
in the precipitate of the T687A mutant when detected by P-Thr
antibody or silver stain (Figure 2A and data not shown). Although
a few additional PSF-associated proteins were also observed to
react with the P-Thr antibody, only the 135 kD species differs in
association between the T687A and T687D mutant (see
Figure S1A available online). As the T687A mutant mimics the
dephosphorylated state of PSF that is observed in activated
cells, we reasoned that differential association with the 135 kD
protein could explain differential RNA-binding capability in
resting and activated cells. We therefore subjected the 135 kD
band to mass spectroscopy analysis and identified specific
peptidesfor twonuclear proteins,
(Figure S1B), which comigrate in SDS-PAGE and share
extensive sequence homology (Figure 2B). Using specific
antibodies, we confirmed that both TRAP150 and BTF
coprecipitate with PSF fromnuclear extract and that thisinterac-
tion is reduced in the T687A mutant whencompared to either the
phosphomimic T687D or wild-type PSF (Figure 2C). Consistent
TRAP150 andBTF
Figure 2. Phosphorylation of PSF on T687 Regulates Association with TRAP150/BTF
(A) Anti-P-Thr blot of Flag-PSF immunoprecipitates as in Figure 1C, looking at coprecipitated proteins of around 150 kD.
(B) Schematic of TRAP150 and BTF showing location of RS (yellow) and homologous (orange) domains.
(C) Immunoprecipitates of Flag-PSF variants, similar to Figure 1C, blotted with antibodies specific to TRAP150 or BTF.
(D) Immunoprecipitates of Flag-PSF from resting (?PMA) versus stimulated (+PMA, 72 hr) JSL1 cells, blotted with antibodies specific to the indicated proteins.
Samples in lanes 6 and 7 were treated with 20 U RNase T1 and 20 mg RNase A for 15 min at 37?C prior to IP. Lanes 1 and 2 correspond to 30% input. See also
Figure S1.
Molecular Cell
TRAP150 Binds to PSF to Limit Splicing Activity
128 Molecular Cell 40, 126–137, October 8, 2010 ª2010 Elsevier Inc.
Page 4
with reduced phosphorylation of T687 upon T cell stimulation,
the interaction of PSF with TRAP150 and BTF is also significantly
stronger in resting (?PMA) versus activated (+PMA) JSL1 cells
(Figure 2D). Importantly, we observe no significant change in
the threonine phosphorylation status of TRAP150 itself upon
PMA treatment (Figure S1C). Furthermore, the interaction
between PSF and TRAP150/BTF in resting cells is independent
of RNA, as addition of RNase A and T1 did not decrease the
observed coprecipitation (Figure 2D, lane 4 versus 7). In fact,
we observe a reproducible increase in the association of
TRAP150 and BTF with PSF following RNase treatment, sug-
gesting that loss of RNA association makes PSF more acces-
sible to bind TRAP150/BTF. Finally, we note that the interaction
of TRAP150/BTF with PSF is unlikely to be directly mediated by
T687, as deletion of the C-terminal 40 amino acids of PSF,
encompassing T687, is also permissive for interaction with
TRAP150 and BTF (Figure 2A, Figure S1D). We hypothesize,
therefore, that phosphorylation of T687 drives association with
TRAP150 and/or BTF in an allosteric manner as discussed
The function of both TRAP150 and BTF remains poorly char-
acterized. TRAP150 was initially cloned as a component of the
transcription mediator complex (Fondell et al., 1996). However,
consistent with subsequent studies suggesting that TRAP150
is not a primary mediator subunit (Conaway et al., 2005), we
do not observe an interaction of PSF with TRAP220, a core
component of the mediator complex (Figure 2D). More recent
studies have implicated TRAP150 and BTF in RNA processing
(Merz et al., 2007), either as components of a complex with
SNIP1 involved in mRNA stability (Bracken et al., 2008) or in
a loosely associated complex of splicing regulatory factors
including nucleolin and hnRNP A1 (Li et al., 2003). We do detect
SNIP1, nucleolin, and hnRNP A1 in precipitates with PSF.
However, unlike TRAP150 and BTF, these proteins associate
with PSF equally in resting and stimulated cells, and the addition
of RNase completely abolishes this interaction (Figure 2D).
Together these data demonstrate a specific and phosphoryla-
tion-dependent interaction of TRAP150/BTF with PSF and
suggest that this TRAP150-BTF-PSF complex is distinct from
other assemblies in which TRAP150 or BTF have been charac-
terized thus far.
Interaction of TRAP150 with PSF Inhibits CD45 Exon
Toinvestigate thefunctionalroleof thePSF-TRAP150-BTF inter-
action with respect to CD45 alternative splicing, we next
knocked down TRAP150 and BTF using morpholino oligos.
Remarkably, knockdown of TRAP150 results in a dose-depen-
dent decrease in CD45 variable exon inclusion in resting cells,
which at the highest level of TRAP150 depletion (Figure 3A) is
similartothatobservedupon Tcell activation(+PMA).This effect
of TRAP150 depletion on CD45 alternative splicing is dependent
on the presence of the ESS1 regulatory element through which
PSF functions as inclusion of CD45 variable exon 4 from a stan-
dard minigene construct is decreased upon depletion of
TRAP150, while splicing of an analogous construct lacking the
ESS1 regulatory sequence is unaffected by TRAP150 knock
down (Figure S2A). Notably, however, this change in splicing
occurs without any alterations in the expression of the ESS1
regulatory proteins, PSF, hnRNP L, or hnRNP LL (Figure S2B).
To initially investigate if the ESS1-dependent splicing change
we observe upon TRAP150 depletion is a result of altered
TRAP150-PSF interaction, we also tested the effect of disrupting
this interaction by mutating PSF. We predict that the T687A
mutant of PSF, which interacts more weakly with TRAP150
(Figures 2A and 2C), should promote CD45 exon repression in
a manner similar to that observed upon knockdown of
TRAP150. Strikingly, we observe a significant decrease in the
inclusion of wild-type CD45 exon 4 upon overexpression of
PSF-T687A in resting cells (Figure 3B). In contrast, expression
of WT PSF had no effect on exon 4 splicing, and neither protein
mimic PSF-T687D had no effect on exon 4 splicing in either
resting or stimulated cells (Figure 3B and data not shown),
consistent with the notion that the T687 phosphorylated pool of
PSF is itself nonfunctional for CD45 exon repression and does
not block the accumulation and activity of the hypophosphory-
lated form of endogenous PSF upon stimulation (see below). A
functional role for the TRAP150-PSF interaction is further sup-
ported by the finding that the majority of wild-type, endogenous
PSF is associated with TRAP150 in resting cells, as indicated
by the efficiency of coimmunoprecipitation (Figure 3C). In
contrast, the bulk of PSF is not precipitated by TRAP150
following stimulation (Figure 3C). Taken together, these data
confirm a significant and regulated interaction between endoge-
nous TRAP150 and PSF, and are consistent with this interaction
playing a critical role in regulating PSF function in CD45 splicing.
not substantially change CD45 alternative splicing either on its
own (Figure S2C) or in combination with TRAP150 depletion
(data not shown). As BTF and TRAP150 have been shown to
associate (Bracken et al., 2008), we reasoned that TRAP150
could be the functionally relevant PSF binding partner, with
BTF only recruited to PSF indirectly via TRAP150. Consistent
with this prediction, we find that upon knockdown of TRAP150,
BTF no longer coprecipitates with PSF, although total protein
levels remain unchanged (Figures S2B and S2D). In contrast,
the association of TRAP150 with PSF does not require BTF, as
knockdown of BTF does not weaken TRAP150 coprecipitation
with PSF (Figure S2D).Moreover,we observe a direct interaction
in vitro between purified recombinant TRAP150 and PSF (data
not shown). Therefore we conclude that TRAP150 is the primary
functionally relevant PSF-associated protein and have focused
our subsequent studies on characterizing the regulation and
consequence of this TRAP150-PSF interaction.
TRAP150 Directly Inhibits Binding of PSF to the ESS1
Theaboveresults,together withourpreviousdemonstration that
T cell activation induces PSF's association with the ESS1 regu-
latory sequence in CD45 (Melton et al., 2007; Motta-Mena et al.,
2010), suggest a mechanism in which the interaction between
TRAP150 and PSF limits exon skipping by blocking the interac-
tion of PSF with ESS1. We therefore assayed the association of
PSF with the ESS1 regulatory element in an RNA pull-down
Molecular Cell
TRAP150 Binds to PSF to Limit Splicing Activity
Molecular Cell 40, 126–137, October 8, 2010 ª2010 Elsevier Inc. 129
Page 5
an increase in the association of PSF with ESS1 RNA that is
highly similar to that observed upon T cell activation (Melton
et al., 2007; Motta-Mena et al., 2010). Moreover, PSF associated
with ESS1 RNA does not react efficiently with the P-Thr antibody
unless TRAP150 is depleted (Figure 4A, P-Thr), suggesting that
the phosphorylated population of PSF, bound by TRAP150, is
hindered from binding to ESS1. In further support of this model,
we are unable to detect any TRAP150 itself, or BTF, among the
ESS1-associated proteins (Melton et al., 2007; and data not
We next sought to determine whether TRAP150 directly regu-
lates the binding of PSF to the CD45 ESS1 RNA using purified
proteins (Figure S3A). Purified PSF binds specifically to radiola-
beled ESS1 RNA in a UV crosslinking assay, as shown by
competition with nonspecific versus ESS1 RNA (Figure S3B).
Importantly, PSF purified from resting or stimulated cells binds
equally well to ESS1 RNA, demonstrating that the RNA-binding
capability of PSF itself is not altered upon stimulation
(Figure 4B). However, the addition of purified TRAP150 specifi-
cally inhibits the RNA-binding capability of PSF purified from
resting cells (Figure 4B, top), with little impact on the RNA
binding of PSF purified fromstimulated cells (Figure 4B, bottom).
We further observe that nuclear extract differentially inhibits
RNA bindingof PSFfrom
(Figure 4B). This result provides an explanation for our previous
observation that PSF purified from resting cells cannot repress
CD45 exon usage when added to nuclear extract in an in vitro
splicing assay (Melton et al., 2007), as the nuclear extract would
nuclear extracts from resting or activated T cells are equally
effective in inhibiting RNA binding of PSF, demonstrating that
stimulation alters the inherent susceptibility of PSF to binding
extract (Figure 4B and Figure S3C). Moreover, the inhibitory
against TRAP150, but not by a variety of control antibodies (Fig-
ure 4C and data not shown). Taken together, our data from inter-
action, knockdown, and RNA-binding studies demonstrate that
the phosphorylation-dependent
directly blocks PSF from binding ESS1 in resting T cells, thereby
limiting the repression of CD45 exons prior to T cell activation.
restingand activatedcells
TRAP150-PSF interaction
Figure 3. The Majority of Cellular PSF Is Bound to TRAP150 to Inhibit CD45 Exon Skipping in Resting Cells
(A) Representative RT-PCR and quantitation (n = 3) of CD45 isoform expression in JSL1 cells under resting (?) or stimulated (+PMA, 72 hr) conditions or after
treatment of resting cells with control or anti-TRAP150 morpholino oligomers. Error bars correspond to standard deviation. Western blot confirmation of
TRAP150 knockdown shown in lower right.
(B) Quantitation (n = 4) of splicing of CD45 exon 4-derived minigenes in cells transfected with the WT, T687A, or T687D version of Flag-PSF (see western). Error
bars correspond to standard deviation, p value for difference between WT and TA PSF is shown. Minigene E4-WT contains the complete native CD45 exon
4 sequence, while in E4-DESS the ESS1 motif has been replaced by an unrelated sequence of similar length (Melton, et al., 2007).
(C) Analysis of PSF in pellet (P) versus supernatant (S) following precipitation with antibody to TRAP150 or control antibody (IgG). See also Figure S2.
Molecular Cell
TRAP150 Binds to PSF to Limit Splicing Activity
130 Molecular Cell 40, 126–137, October 8, 2010 ª2010 Elsevier Inc.


text:
Let's say I have an array like this:
string x[2][55];
If I want to fill it with "-1", is this the correct way:
That crashed when I tried to run it. If I change x[2][55] to x[1][54] it works but it doesn't init the last element of the array.
Here's an example to prove my point:
string x[2][55];
x[1][54] = "x";
cout<<x[1][54]<<endl; // this print's "x"


text:
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Date: 1936 ca.
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text:
9 duplexes / 9 twins
by Everett Williams
These nine duplexes reside in South Brooklyn, NY. Over time each unit begins to distinguish itself from its "siamese" twin. These photographs document the twins' during the summer of 2008—about 60 years from their likely date of birth.
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text:
Pakistan Veterinary Journal Prevalence of Brucellosis in Cattle in Urmia, Iran
ABSTRACT This study was carried out to know the prevalence of brucellosis in Urmia, Iran. For this purpose, 338 milk samples were collected from 36 villages in two seasons (spring and autumn, 2008). In spring, 82 milk samples from 11 villages and in autumn, 256 milk samples from 25 villages were collected randomly. These samples were examined for Brucella abortus antibodies with milk ring test. From 82 milk samples collected during spring, 1 (1.22%) showed positive while others (98.78%) showed negative reaction. From 256 milk samples collected during autumn, 3 (1.17%) showed positive and 253(98.83%) showed negative reaction. It was concluded from the results that prevalence of brucellosis in cattle was low in this region.
Full-textDOI: · Available from: morteza Haghi, Sep 28, 2015
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Article: A Review of Serological Investigations of Brucellosis Among Farm Animals and Humans in Northern Provinces of Iraq (1974?2004)[Show abstract] [Hide abstract]
ABSTRACT: Brucellosis is an endemic anthropozoonotic disease in most Middle East countries including Iraq. It causes considerable loss of economy and energy, particularly in the rural areas. Since 1966 when the prevalence of brucella agglutinin from slaughtered sheep and goat was reported in Baghdad, several trials have been conducted including serological studies of brucellosis in the northern provinces of Iraq (Nainava, Duhok, Erbil, Kirkuk and Sulaimani). Humans, sheep, goats, cattle and buffalo have been involved. The studies also included isolation and identification of Brucella species, including B. abortus, B. melitensis and B. ovis. Local veterinary authorities, international organizations, universities and some NGOs have been involved. Moreover, limited efforts have been reported concerning the control and eradication of the disease among goats and sheep in some restricted areas. A limited vaccination program for newborn lambs and kids also has been conducted by the FAO, which was involved in the veterinary emergency plan for brucellosis in 2000. Northern provinces share an extensive border with Iran, Turkey and Syria, in addition to the direct and intimate contacts with other provinces of Iraq, which share borders with Jordan, Saudi Arabia and Kuwait. Hence any strategic planning for the control or eradication of this devastating anthropozoonotic disease in the country should be implemented regionally and not locally.Journal of Veterinary Medicine Series B 01/2007; 53 Suppl 1(Suppl 1):38-40. DOI:10.1111/j.1439-0450.2006.01021.x · 1.57 Impact Factor
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Article: An overview of the epidemiology and epizootology of brucellosis in selected countries of Central and Southeast Europe[Show abstract] [Hide abstract]
ABSTRACT: The objective of this paper is to give an overview of the epidemiologic and epizootic status of brucellosis in selected countries of Central and Southeast Europe (Balkan region). Based on dimension of the disease problem, there is a need to establish collaboration in the eradication and prevention of brucellosis between all countries in the region. Although there were no readily accessible data concerning epidemiology and epizootology of brucellosis in these countries, the limited official and published data were analyzed. The incidence of brucellosis caused by Brucella melitensis in sheep, goats and humans is a very significant problem in Macedonia and Greece. In Greece, cattle are also affected either by B. melitensis or B. abortus. The disease is an endemic problem in some regions of Yugoslavia and includes B. suis biovar 2 in pigs and in Croatia, B. melitensis in sheep, goats and human is found occasionally. No problem appears to exist with brucellosis in Bulgaria. Financial well-supported brucellosis control programs of the European Union that will include all countries, regardless of the magnitude of brucellosis incidence, are needed for eradication and control of brucellosis.Veterinary Microbiology 01/2003; 90(1-4):147-55. DOI:10.1016/S0378-1135(02)00250-X · 2.51 Impact Factor