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micoach is all you need to get the results you want.
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it's very hard to find decent fitting women's shorts that aren't showing your butt, so I have to get men's short which are usually too big and bulky. These are actually pretty nice. They are a touch long, and a little too wide, but that's to be expected as a woman buying men's shorts. they are very comfortable and work well out on the soccer fields. ... read more
I bought these shorts along with the 365 hoodie, and the only bad point is the one, singular - extremely small pocket on the rear right side. I can barely fit my phone inside, it's extremely tight. If you're looking for a product with pockets, this one isn't for you. Despite that, I really like the product, especially to play in. Again they're very light, the aesthetics are fantastic and the quality from Adidas is yet again second to none. ... read more
I mainly bought it because of the refined and beautiful pattern. Shipping was faster than expected, I did not realize the item arrived the day after they sent it out. The only con is it is a bit on the larger size for a S. The short goes all the way to knee level and I am 5'10". I hoped it will be a bit shorter than that but I also know it's a typical design for training short. Still like it! ... read more
Bought the green pair about 1 month ago. Great color. These shorts are nice free moving light short. Very comfortable for any type of activity. I have never had a pair of Adidas shorts I didn't love, that's why I keep coming back. That said, what I like about these more than any of the others is the length 9". Right in between the standard length for the Response shorts at 7" and the Aeroknit and the Climachill at 10". For me, this is the perfect length. 6'2" 190lbs w 33" inseam. ... read more
These shorts are great. I have always worn adidas soccer gear. The material and light weight feel is a testament to their great product and evolution. Perfect for playing soccer, or just any physical activity! ... read more
I have just bought 2 pairs of these shorts, the others are the black ones and they are a really comfortable, lightweight short for running in. I would not hesitate to buy other pairs in different colours if they were available ... read more
These climacool shorts are perfect for jogging & gym. Easy wash and wear, quick to dry, no ironing or pressing needed. The iconic Adidas stripes make them extra-special and distinctive. The handy zip-pocket is essential for keys, cards & discreet items. ... read more
I really wanted to like these, because I always wear shorts with long liners. The shorts themselves are great, light and airy, dried quickly, cool. But the liners....ugh. Formless and not very good at containing things. I was constantly tugging and pulling them up, then pulling them down. Geez, Adidas, did a real guy try these on before you started making them? Anyway, true to size, but only a C+. Wearable but not all that great. Don't think I'll buy another pair. Too bad. ... read more
I have bought these shorts because I love the Adidas Response range. They are a good quality short, fit great and a very comfortable. Great for running, gym, lounging around in the sun or tale on holiday. Hope there are more colours. Find the men's range of running gear can be quite boring. But love these shorts. Will be getting the black ones. ... read more
Ordered these shorts in a large due to being a 34 waist, they are massive, I have to turn over the waistband and they look very baggy . I should of got the medium, bad mistake on my behalf. The shorts are very good, I only buy Adidas for the quality, so I was surprised at the size due to having other pairs of Adidas shorts in a large that fit well. Can't understand why they are sent in a shoe box,think of the environment, use packaging that suits the item being sent. ... read more
I wear these for training in the gym and I've never seen anyone wearing anything close to this colour, which I really like! It's good to look different and the material is great for work out gear. They are comfortable, wash well and there are matching t-shirts and pants to complete the look. Highly recommended! ... read more
They look good and feel good. However, the pockets are to shallow and small. I always listen to music from my IPhone 5 while I train. It falls out of my pocket all the time. I would never casually wear these in public with a wallet and car keys in my pocket. ... read more


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Eagan, MN (Silver Bell Rd)
1985 Silver Bell Rd
Eagan, MN 55122
Join with us in October and receive a FREE 30-day workout program, 3 personal training sessions and daily motivational emails!! Call 651-686-4667 or stop in for details
valid through: oct 31, 2015
Refer 5 friends during the month of October and receive 6 months of dues for FREE!
club at a glance
24-hour access
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anywhere club access
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personal training
cardio tvs
health plan discounts
small group training
cardio equipment
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john schultz
We are so excited to welcome you to our club! I discovered my love for fitness as a life-long athlete. Growing up, I played hockey as soon as I could walk and played football into college. I have a passion for fitness and helping people strive to be a better person then they were yesterday. I love coming to work and supporting all member's and staff achieve a healthy, happy lifestyle!
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It's a shame that the few bad apples in sports often lead the headlines. Some guys can't live without being the center of attention. They act out, they act better than everyone or they simply just act stupidly. And it all begins upstairs. Some of these athletes are extremely calculated with their actions and others are lucky that they remember to wake up in the morning.
It takes a lot of work to be a true fan of these guys because it would seem that you'd spend most of your fandom explaining or apologizing for them. IASID gives you the ten biggest headcases in sports.
10 - Ndamukong Suh - The Lions defensive end is extremely meticulous in choosing his words in interviews. He's book smart (graduated from Nebraska with a degree in Engineering), but acts like he's the smartest in the room. It's tough to buy it after seeing him take so many cheapshots at opponents, including "the stomp."
9 - Milton Bradley - A current free agent who hit .218 with the Mariners last season, Bradley is a hot head of all hot heads. His best/worst display was tearing his ACL while arguing a play at first base.
8 - Ozzie Guillen - He's a walking quote and most of them contain some sort of hatred. Perhaps his best is calling himself, "the Charlie Sheen of baseball minus the drugs and the prostitutes." His act grew tired in Chicago and a mutual parting led him to Miami. He talks so darn much, he might need suntan lotion for his tongue.
7 - Terrell Owens - Despite being 38 years old, T.O. could probably still help a team as a third or fourth wide receiver. But he's been eschewed by NFL teams and found himself playing in an indoor league because of his attitude. He's a drama king wherever he goes and if the spotlight isn't on him now, it will be soon enough. Maybe the demotion and the foreclosures have humbled him, but I wouldn't bet on it.
6 - Chad Ochocinco - He used to be Chad Johnson, the wide receiver known for making plays with Carson Palmer. Then he became a wide receiver who had outlandish touchdown celebrations. Then he became the wide receiver known as Chad Ochocinco and a social media *****. The guy posted a picture of himself playing a slot machine in his boxers. Then he became an irrelevant member of the Patriots. Chad, it's your move.
5 - Manny Ramirez - "Manny being Manny" has become the biggest excuse in sports. In layman's terms, it means "please excuse the actions of that grown man even though a six year old monkey would know better."
4 - Metta World Peace - He changed his name to Metta World Peace.
3 - Nyjer Morgan - He's the only man in baseball with an alter ego. Nobody knows when "Tony Plush" will come out and it's scary because I don't think he does either.
2 - LeBron James - LBJ is the most physically imposing player in the NBA when compared to guys who play the same position. He's bigger and stronger than guards and he's quicker than forwards. He's probably the most talented player in today's game and in the history of the sport. But, he's a mess between the ears. Aside from his 25 straight points against the Pistons in the 2009 playoffs, he hasn't proven to be a winner. It was most recently exemplified when he passed up a chance to take the game-winning shot at the end of the All Star game. Instead, he threw it away trying to pass it off.
1 - Floyd Mayweather - The more this guy opens his mouth, the dumber we get. Jeremy Lin has been hyped only because he's Asian. Manny Pacquiao isn't the sharpest knife in the drawer (kettle: hi pot, you're black). And he wanted to throw down with Larry Merchant. Clueless.
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Multi-Family Sales Rep Job
Time Warner Cable - Palmdale, CA
This job posting is no longer available on Time Warner Cable. Find similar jobs:Multi Family Sales Representative jobs - Time Warner Cable jobs
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Time Warner Cable (TWC) makes coaxial quiver. The company is the #2 US cable company, after Comcast, with operations in 28 states. Its core...


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March 3, 2012
Texas Tech quickly opened the scoring with a pair of runs in the top of the first inning. The Red Raiders collected a pair of base hits and stole a total of three bases that led to both scores. The Red Raiders were looking to break the game wide-open by sending eight men to the plate in the top of the first, but Owl starter Matthew Reckling worked out of the jam down 2-0.
Reckling's teammates on the offensive side answered with three runs after sending just three batters to the plate in the bottom of the frame. Christian Stringer drew a leadoff walk and Michael Fuda followed with a double to put men on second and third. Michael Ratterree then knocked three-run homer to Minute Maid Park's Crawford Boxes in left field. His second round-tripper of the season gave the Owls a 3-2 lead.
The Blue & Gray added two more in the second. Derek Hamilton led off with a single, moved into scoring position on a wild pitch and to third on a ground out. Geoff Perrott executed a text-book bunt for a base hit, but a Red Raider fielding error on the play allowed Perrott to reach all the way to third. The Owls still had the same situation with a runner on third and one away, and this time Stringer executed an identical bunt for a sacrifice and an RBI that made the score 5-2.
"I think Matthew did a good job righting-himself, and we put that splash of offense in," said Rice head coach Wayne Graham, now in his 21st season at the helm of the Owl program. "The bunts were really good for a change. We needed the safety squeeze, it's a big part of our offense and tonight we executed it well for two runs. The offense is there, they just have to keep fighting through and get some quality at bats."
In the bottom of the fifth Rice stretched the margin to four with the final run of the contest. Ratterree lined a one-out single to right-center. He scored from first base on Rathjen's two-out double that bounced off the wall in left-center that bounced in front of the home bullpen.
Reckling meanwhile had settled into a groove on the mound. After the top of the first the senior right-hander from Houston did not allow any hits again until the seventh and he recorded three 1-2-3 innings. He finished the night working 6.2 innings, allowing two runs on four hits and three walks while striking out three.
"That first inning was pretty rough, but Coach Graham always tells us, `be an unshakeable pitcher,'" said Reckling. "I don't think I had my best stuff tonight, but I knew I needed to get us some innings. I'm not pleased with my outing, but there's some positives to build on. It was the offense that really picked me up and got things going."
Texas Tech tried to stage a two-out rally in the top of the seventh. The Red Raiders loaded the bases on a walk and two hits, prompting the Owls to call down to the bullpen. Junior right-hander Tyler Duffey entered the pressure-filled jam and promptly struck out the first Tech batter he faced on three pitches to end the inning. Duffey went on to post a 1-2-3 eighth and strong ninth on the way to his second save of the season. He struck out four without allowing any hits in 2.1 innings of relief.
Fuda and Hamilton shared the game-lead with three hits each. Ratterree's three RBI tied his season-high.
Rice improved to 10-1, a record which is the program's best start since the national championship season of 2003. The Red Raiders dropped to 8-2 with only the team's second loss of the young season.
Tournament action at the College Classic concludes on Sunday (Mar. 4). The Owls next meet Tennessee in the event's finale. First pitch with the Volunteers is set for 6:05 pm. The contest will be broadcast on ESPN3.
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Summer Ball Update
07/20/2015 - Players on the Rice baseball team are staying busy this summer competing in a host of collegiate leagues across the nation.
Summer Ball Update
07/13/2015 - Rice baseball players are staying busy this summer playing in a host of collegiate leagues across the country. Here's a weekly look at the Owls'...
Holt Named A Major League All-Star
07/07/2015 - Former Rice Owl Brock Holt, currently of the Boston Red Sox, was named to the American League All Star team for next week's mid-summer classic in...
Rice Announces 2015 Hall of Fame Class
06/30/2015 - Richard and William Barker, Bubba Crosby, Jarett Dillard, Funmilayo Jimoh, Holly Jones-Wright, Lauren Shockley and Brent Scott will be inducted as ...
Berkman Inducted Into College Baseball Hall of ...
06/30/2015 - Rice great Lance Berkman was part of a star-studded group inducted into the College Baseball Hall of Fame on its "Night of Champions" in Lubbock,...
Reeves Named ABCA Third Team All-America
06/14/2015 - John Clay Reeves was named a third-team All-America selection by the American Baseball Coaches Association (ABCA), the organization announced from ...
A Very Cool Draft
06/10/2015 - Rice's Kevin McCanna, Austin Orewiler, Matt Ditman, John Clay Reeves and John Williamson were all selected on the final day of the of the 2015...
Stephens & Byrd Selected Tuesday In Major...
06/09/2015 - Rice's Leon Byrd and Jordan Stephens were both selected on Tuesday's second day of the 2015 Major League Baseball amateur draft.
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Creating True Value Proposition In Clean Tech
Watch Now
Date Added: Apr 2010
Format: Webcast
Improved safety and water quality aren't the only results of displacing chlorine as a water purifier. Carlos Perea, Miox CEO, points out that saving money is the main reason that his private and municipal customers utilize his company's water treatment capabilities. He states that Miox's focus is always on driving costs down, because without this edge - no matter how honorable a clean tech innovation - a disruptive technology cannot ever achieve real market dominance. If an innovation in power or natural resources isn't going to be subsidized or regulated, it still must abide by the structure of the free market, and its relative cost is imperative for its success.


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Nerds, be PROUD! Portland is #2!!
by Iris Harrison
posted Apr 10 2013 3:38PM
I think we humans are all sort of nerds about something. There's at least one thing in our lives that others would probably consider uncool. I'm a garden nerd. Also a Monty Python nerd. There's other things, but I just basically wanted to set up a reason to read this cool blog.
Portland came in at #2 in the "10 Nerdiest Cities in America" and I think that's so cool. Not only has Portlandia put us on the map for being weird/nerdy/cool, but now this.
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Illinois Public Media
All articles tagged Eavesdropping
Lawmakers Work To Replace Illinois' Eavesdropping Law
Illinois Supreme Court: Eavesdropping Is Unconstitutional
NSA's Reach Leads To Calls For Updated Eavesdropping Laws
Eavesdropping Bill Advances to Illinois House
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WILL Highlights
Vintage Vinyl is coming!
The Vintage Vinyl Sale to benefit Illinois Radio Reader returns on October 3. Click for the latest info.
WILL News wins PRNDI Award for "Unmet Needs" Radio Doc
The 2014 radio documentary, produced by former WILL staff and hosted by Scott Cameron, won second place in the Long Form Documentary category among medium-sized stations.
WILL Ag Director Dave Dickey retires
He carried on the farm broadcasting tradition, while innovating on the Internet.


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Back to Previous Page
The Boss 302 returns to the road after 42 years.
Shaking Hands With The Devil
In this video the Flex picks off a modded Mustang GT.
Make Your "Five-Oh" Go Faster and Smoother
If you love Ford performance, this is the place for you!
King of the Road
A Race Car For The Streets
Born on California's highways, the Mustang SSP offered police a high-performance alternative.
How to get up-close and personal to find the finest Ford Mustang you can afford.
A visual guide to telling one vintage Pony Car from its model-year stable mates.
A visual feast for Mustang lovers.
Mustang's third body style emerges.
A Real Jekyll & Hyde Ride
Daytona Speedway was a fitting corral for these ponies.
Upgrading a 1966 Mustang's Baer system from two-piston PBR caliper to six-piston Baer caliper.
Tulsa Raceway Park thundered with Ford power!
Carving corners with Ford power!
Walking around the paddock at Hallett Motor Racing Circuit
The Ultimate Ford Family Reunion
Fords invade downtown Tulsa's Brady Arts District
The power plants that gave the Mustang its gallop.
A great mix of old and new meet at this year's Atlanta auto show.
Walking around Tulsa Raceway Park
The capstone of a great event.
Families, fun and Mustangs at Myrtle Beach.
It is as if Ford designed the new Mustang to be modified.
The Florida heat had nothing on these paint jobs.
Variety was the name of the game as the Festivals of Speed returned to Amelia Island.
These kids show what the next generation can do!
These builders went "all in" on their designs and it shows!
A new tradition begins that will surely become a fan favorite.
Don't be fooled, it takes a lot of work to look this cool.
Steel may be real, but fiberglass has class!
A fitting finale for an amazing Ford filled event!
Overcoming mechanic's fear.
AutoTrader Classics Editor Brian Medford travels to Streetside Classics in Powder Springs, GA to check out their inventory and talk about some of their cars.
From horseless carriages to speedsters, these rolling masterpieces captured everyone's imagination.
Race Track Refugees On Display
Records fell with the gavel in Gainesville, Georgia.
Brutally effective in their design, these retired racers also hold a certain beauty.
Steel may be real, but this plastic is fantastic.
Taking a walk around the pits at Tulsa Raceway Park.
It isn't very often that an attraction exceeds expectations by leaps and bounds, but the NASCAR Hall of Fame does so at every turn.
These trucks haul more smiles to the mile.
More kings of the street and strip.
These cars are prime examples of what the automotive craft can be when we dare to dream.
Sometimes an auction is also a car show.
The Eagle has landed,...finally. Infiniti's FX35 finishes what AMC started more than three decades ago.
The true root of the hobby, these hot rods hit the jackpot.
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Latest: Coming Soon from Lumenera: INFINITY3S-1UR
Latest: The Importance of Stringent Complete Response (sCR) in Multiple Myeloma
Latest: Agilent Technologies Shares Vision for Precision Genomics at ASHG 2015
Latest: Mettler-Toledo Boosts Food Safety with Next Generation Bulk Flow Inspection
Latest: PlasmaQuant ® MS — Accurate and Precise Arsenic Speciation in Foods and Beverage with Sup...
Latest: Phenomenex Strata™-X µElution SPE Plates Enable Cost-Effective Cleanup of Small Volume Sa...
Latest: Live Demos & New Learning: Lab Innovations 2015
The Importance of Stringent Complete Response (sCR) in Multiple Myeloma
Download our New Mass Spectrometers Buying Guide
Mettler-Toledo Boosts Food Safety with Next Generation Bulk Flow Inspection
Coming Soon from Lumenera: INFINITY3S-1UR
10 Traits of a Science Lab Superhero
Optimize Your Pesticide Detection and Quantitation
High Resolution and Rapid Peptide Mapping of Monoclonal Antibody Using an Agilent 1290 In...
Amniocyte Derived Human Cell Line CAP-T® is a Versatile Host for Transient Bulk Transfect...
DRAQ5™ in FC-Based Apoptosis Assays: Far-Red Fluorescent Live-Cell Permeant DNA Dye
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Solvent Exchange from Dichloromethane to n-Hexanes Using the DryVap® Concentrator System
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In this application note, the AOAC official method 2007.01 for pesticide residues in foods is described for the analysis of 18 peptides and 2 internal standards from spinach using a Phenomenex roQ QuEChERS extraction kit and a PSA/GCB dSPE kit for sample preparation. Analysis was performed by LC/MS/MS and GC/MS. The roQ PSA/GCB dSPE kit was able to quickly remove pigment and other matrix interferences from the difficult spinach sample. Recoveries of all 18 pesticides were between 70% and 120% with sample-to-sample variation of <15%. Such results demonstrate the effectiveness of the roQ QuEChERS product for food analysis.
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Kotimainen Liina is a very strong cotton twine with multiple plies. Use this sturdy Finnish 15-ply yarn as your warp for items that need extra strength like rag rugs. Available in 1.1lb cones. Made in Finland.
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Page 1
Cdc7p-Dbf4p Regulates Mitotic Exit by Inhibiting Polo
Charles T. Miller1,2, Carrie Gabrielse2, Ying-Chou Chen2,3, Michael Weinreich2*
1Graduate Program in Cell and Molecular Biology, Michigan State University, East Lansing, Michigan, United States of America, 2Laboratory of Chromosome Replication,
Van Andel Research Institute, Grand Rapids, Michigan, United States of America, 3Graduate Program in Genetics, Michigan State University, East Lansing, Michigan, United
States of America
Cdc7p-Dbf4p is a conserved protein kinase required for the initiation of DNA replication. The Dbf4p regulatory subunit
binds Cdc7p and is essential for Cdc7p kinase activation, however, the N-terminal third of Dbf4p is dispensable for its
essential replication activities. Here, we define a short N-terminal Dbf4p region that targets Cdc7p-Dbf4p kinase to Cdc5p,
the single Polo kinase in budding yeast that regulates mitotic progression and cytokinesis. Dbf4p mediates an interaction
with the Polo substrate-binding domain to inhibit its essential role during mitosis. Although Dbf4p does not inhibit Polo
kinase activity, it nonetheless inhibits Polo-mediated activation of the mitotic exit network (MEN), presumably by altering
Polo substrate targeting. In addition, although dbf4 mutants defective for interaction with Polo transit S-phase normally,
they aberrantly segregate chromosomes following nuclear misorientation. Therefore, Cdc7p-Dbf4p prevents inappropriate
exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint.
Citation: Miller CT, Gabrielse C, Chen Y-C, Weinreich M (2009) Cdc7p-Dbf4p Regulates Mitotic Exit by Inhibiting Polo Kinase. PLoS Genet 5(5): e1000498.
Editor: Orna Cohen-Fix, National Institute of Diabetes and Digestive and Kidney Diseases, United States of America
Received December 4, 2008; Accepted April 29, 2009; Published May 29, 2009
Copyright: ? 2009 Miller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Van Andel Research Institute and American Cancer Society grant RSG0506301GMC to MW. The funders had no role in
study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
* E-mail: email@example.com
Accurate ordering of cell cycle events is an important
requirement for the viability of all eukaryotic organisms. Once
cells commit to duplicate their genome they must restrain mitosis
until replication is complete and then accurately coordinate
mitosis with cytokinesis to ensure the faithful transmission of
chromosomes to daughter cells [1]. Importantly, errors in cell
cycle checkpoints that enforce this ordering can be deleterious for
accurate chromosome transmission. For instance, DNA damage or
replication fork arrest during S-phase elicits a reversible block to
mitotic progression by the budding yeast Mec1p (HsATR) and
Rad53p (HsChk2) checkpoint kinases [2,3]. In the absence of
Mec1p or Rad53p, replication fork arrest during S-phase is not
sensed leading to premature mitotic events and cell death
(reviewed by [4]). Additionally, since daughter cell growth is
highly polarized in the budding yeast, exit from mitosis is
prevented until sister chromatids segregate through the bud neck
and into the daughter cell [5–7]. This ensures that spindle
disassembly and mitotic exit are not initiated until accurate
chromosome partitioning between mother and daughter cells has
occurred. Failure to block mitotic exit when nuclear division takes
place within the mother cell results in polyploid and anucleate
progeny [8,9]. It is not surprising therefore, that both entry into
and exit from mitosis are delayed by cellular checkpoints that
respond to replication stress, chromosome damage, or spindle
disruption [1]. Errors in these mitotic checkpoints are catastrophic
and result in ploidy defects and genetic alterations, which are
frequently observed in human cancers (reviewed by [10]).
The Cdc7p-Dbf4p kinase is required to catalyze the initiation of
DNA synthesis at the beginning of S-phase (reviewed by [11]).
Cdc7p kinase activity is tightly regulated during the cell cycle by
binding the Dbf4p regulatory subunit, which is cyclically expressed.
Dbf4p accumulates in late G1, is present throughout S-phase and
then is destroyed during mitosis and early G1 by anaphase
promoting complex (APC)-dependent degradation [12–17]. There-
fore, Cdc7p-Dbf4p kinase activity is low following exit from mitosis
and entry into G1-phase until it is needed to initiate a new round of
DNA synthesis in late G1-phase of the following cell cycle. Multiple
lines of evidence suggest that Cdc7p-Dbf4p activates the MCM
DNA helicase [18–20] that is assembled at origins of replication in
early G1 in an inactive form (reviewed in [21,22]).
In addition to its essential role in replication initiation, several
studies suggest that the Cdc7p-Dbf4p kinase responds to DNA
damage or replication fork stalling but its precise role in these
activities is unknown [17,23–25]. Dbf4p encodes a dispensable
BRCT-like domain in the N-terminus that might target the kinase
to stalled replication forks [26,27]. In fission yeast, the Cdc7p-
Dbf4p ortholog Hsk1p-Dfp1p interacts with Swi1p (budding yeast
Tof1p), a component of replication forks required for fork stability
and also promotes centromeric cohesion in early mitosis [28,29].
Rad53p also phosphorylates Dbf4p in response to replication stress
and this regulation requires N-terminal Dbf4p sequences through
which Rad53p physically interacts [17,25,30]. Interestingly, the
absence of the BRCT-like domain results in a defect in late origin
activation suggesting that this domain might alter Cdc7p-Dbf4p
binding at early versus late replication origins [26]. Together,
these data suggest that the Dbf4p N-terminus encodes non-
PLoS Genetics | www.plosgenetics.org1 May 2009 | Volume 5 | Issue 5 | e1000498
Page 2
essential regulatory functions that target the kinase to particular
To identify proteins that interact with the Dbf4p N-terminus,
we performed a yeast two-hybrid screen with an N-terminal region
of Dbf4p and identified an interaction with the Cdc5p kinase, the
only Polo ortholog in yeast. Budding yeast Polo, like Drosophila
Polo and human Polo-like kinase 1 (Plk1), functions as a master
regulator of mitotic progression and is also required for cytokinesis
(reviewed by [31,32]). Polo activity is regulated by several
independent cellular mechanisms. Polo protein levels are con-
trolled by APC-dependent degradation in mitosis/G1-phase and
activation of Polo catalytic activity requires phosphorylation by
Cdk1 kinase early in G2 [33–35]. In addition, Polo function is
inhibited by cell cycle checkpoints that are induced following DNA
or spindle damage [36–38]. A genetic and physical interaction
between Dbf4 and Polo was described previously [39,40], however
the biological significance of this interaction was not known.
Polo controls multiple mitotic events to ensure accurate
chromosome segregation. After anaphase initiation, Polo is
required to activate the FEAR (Cdc14 early anaphase release)
and MEN (mitotic exit network) pathways that promote nucleolar
release of Cdc14p phosphatase [37,41–43]. Limited Cdc14p
release by the FEAR pathway promotes accurate rDNA and
telomere segregation [44–46]. Subsequent full nucleolar release of
Cdc14p by the MEN reverses Cdk substrate phosphorylation that
leads to APC-Cdh1p activation, cyclin destruction and mitotic
spindle disassembly (reviewed by [47]). Activation of the MEN is
promoted by Tem1p-GTP and antagonized by Bfa1p-Bub2p, a
two-component GTPase activating protein (GAP) [48–50]. To
promote mitotic exit, Polo phosphorylates Bfa1p-Bub2p to inhibit
its GAP activity and is also required for activation of Dbf2p kinase
activity, independently of Bfa1p-Bub2p [37,49,51,52]. The Polo
requirement for Dbf2p kinase activation may reflect that Polo also
promotes Cdc14p release in the FEAR pathway, which primes the
MEN [43]. Therefore, Polo promotes accumulation of Tem1p-
GTP and activation of the downstream MEN kinases Cdc15p and
Dbf2p, which ultimately cause full release of Cdc14p from the
nucleolus. In response to replication fork arrest, Rad53 inhibits
MEN activation, which may or may not impact Polo activity since
the molecular basis of this regulation is not understood [53,54].
Spindle position defects also counteract Polo activity by targeting
Kin4p kinase to the spindle poles where it inhibits Polo-dependent
Bfa1p phosphorylation [8,9,55]. Failure to execute the spindle
position checkpoint (SPOC) results in premature exit from mitosis
and nuclear partitioning defects.
Here we define an N-terminal Dbf4p polo-box interaction
region (that we refer to as the ''PIR'') that binds directly to Polo
and show that Dbf4p inhibits Polo and Dbf2p activity. Deletion of
the PIR allows Cdc14p nucleolar release in a cdc5-1 mutant at the
non-permissive temperature. In response to nuclear misposition-
ing, a dbf4 mutant lacking the PIR fails to arrest in mitosis and
prematurely exits the cell cycle. Thus, Dbf4 protein is required for
proper functioning of the spindle position checkpoint most likely
by antagonizing the ability of Polo to promote Cdc14p release in
either the FEAR or MEN pathways. Our work therefore reveals a
previously unrecognized function for Dbf4p in the regulation of
mitotic progression through a direct interaction with Polo.
The Cdc5p Polo-Box Domain Interacts with the Dbf4p N-
We conducted a yeast two-hybrid screen to identify proteins
that interact with the Dbf4p N-terminus (residues 67–227) and
recovered multiple clones encoding the polo-box domain (PBD) of
Cdc5p. Polo kinase has two conserved domains; an N-terminal
kinase domain and a C-terminal region called the polo-box
domain (PBD) (reviewed by [56]), which is a phospho-Ser/Thr
binding module that targets the kinase to its mitotic substrates
[57,58]. The crystallographic structure of the Plk1 PBD bound to
a phospho-threonine peptide has been solved [59]. Since the
Dbf4p BRCT-like region alone (residues 110–227) failed to
interact with the Polo PBD (Figure 1A), this suggested that the
PBD interaction was occurring through Dbf4p N-terminal
sequences from 67–109. Residues 67–109 were similarly required
for the Polo interaction within the context of full length Dbf4p
(Figure 1B) and were sufficient to interact with the Polo PBD
(Figure 1A). Dbf4p residues 67–109 with all serines/threonines
changed to alanine still interacted with the PBD (Figure 1A)
suggesting that the PBD can bind to this Dbf4p region
independently of phosphorylation. Further deletion and point
mutant analysis (Y-C.C. and M.W., unpublished data) revealed
that residues 82–88 are essential for the Dbf4p-Polo interaction
(Figure 1A, B).
The PBD is composed of three conserved regions called the
Polo-cap (Pc), Polo-box 1 (PB1) and Polo-box 2 (PB2) that fold
together to form a functional phosphopeptide-binding domain
[59]. We deleted conserved residues within the PBD to test their
requirement for interaction with Dbf4p (Figure 1C). Deletion of
residues preceding the PBD (GAD-Polo454–705) had little effect on
the Dbf4p-Polo interaction. However, elimination of the Pc (GAD-
Polo510–705) completely disrupted the interaction with Dbf4p.
These data suggest that the structural integrity of the Polo PBD is
required for Dbf4p binding.
Dbf4p Directly Interacts with Polo
Dbf4p binds and activates the Cdc7p kinase subunit in yeast
and has no known role apart from its interaction with Cdc7p
[14,60]. To determine whether the interaction between Dbf4p and
Polo occurred in the context of the full-length Cdc7p-Dbf4p
kinase, Sf9 cells were co-infected with baculoviruses expressing
Polo, wild type HA-Cdc7p-Dbf4p or wild type HA-Cdc7p with
various Dbf4p deletion derivatives. HA-Cdc7p-Dbf4p kinase was
immunoprecipitated using an antibody against the HA tag and
examined for the presence of Polo. All the Dbf4p deletion
Author Summary
Cdc7p-Dbf4p is a two-subunit enzyme required to copy
the genetic material present on every chromosome in a
process termed DNA replication. Dbf4p is an essential
regulatory subunit of this enzyme that likely directs the
Cdc7p subunit to its targets within the cell. We found that
Dbf4p physically interacts with another protein called Polo
that acts during mitosis, a later step in the cell cycle when
the newly copied chromosomes are equally divided to
mother and daughter cells. Polo is a master regulator of
mitosis and impacts many other proteins required for cell
division. We determined that Cdc7p-Dbf4p is a Polo
inhibitor and, further, that Cdc7p-Dbf4p delayed or
prevented chromosome segregation when errors occurred
during the cell division process. Interestingly, Dbf4p may
bind the Polo substrate-binding domain using a type of
interaction not previously described. Thus, we have
uncovered a new activity for Cdc7p-Dbf4p in the cell
cycle to inhibit chromosome segregation, and these
findings impact multiple fields that investigate how cells
accurately copy and segregate their chromosomes.
Dbf4p Inhibits Polo Kinase
PLoS Genetics | www.plosgenetics.org2May 2009 | Volume 5 | Issue 5 | e1000498
Page 3
derivatives we examined interact with Cdc7p and activate normal
Cdc7p kinase activity ([26] and data not shown). Whereas Polo
interacted with full-length Cdc7p-Dbf4p and Cdc7p-Dbf4-ND65p,
Polo did not interact with Cdc7p-Dbf4p complexes that lacked the
Dbf4p N-terminal 109 residues required for the Polo two-hybrid
interaction (Figure 2A). These data indicate that full length Cdc7p-
Dbf4p kinase interacts with Polo but that Dbf4p residues 65–109
are required for this interaction. Importantly, HA-Cdc7p-Dbf4p
interacts with Cdc5p in yeast when the proteins are expressed at
endogenous levels and this interaction also depends on the Dbf4p
N-terminus (Figure 2B).
We next tested whether Polo bound directly to Dbf4p using
purified proteins. GST-PBD and Sumo-Dbf4p67–109 fusion
proteins purified from E. coli were mixed, pulled down using
glutathione-Sepharose and analyzed by immunoblotting. Al-
though Sumo alone did not interact with GST-PBD, Sumo-
Dbf4p67–109interacted with GST-PBD but not with GST alone
(Figure 2C). These data indicate that Dbf4p residues 67–109 (that
we refer to as the Dbf4p PIR) are sufficient for a direct interaction
with the Polo PBD.
Dbf4p Inhibits Polo Activity
Cdc7p-Dbf4p is required to initiate DNA replication, but is
present throughout S-phase and during the metaphase to
anaphase transition. Dbf4p is subject to APC-Cdc20p dependent
degradation [16] but some protein is still present in late mitotic
mutants that have activated the Cdc20p but not the Cdh1p form
of the APC [17]. We examined Dbf4 protein abundance relative
to Pds1 protein in cells moving synchronously through the cell
cycle, since Pds1p is degraded at the onset of anaphase by APC-
Cdc20 [61]. We found that although the abundance of both
proteins declines at the same time, Pds1p is absent during mitosis
while some fraction of Dbf4p persists (Figure S5). In contrast,
Dbf4p has very low abundance or is absent in cells arrested in G1-
phase by mating-pheromone when the APC-Cdh1p is active
[12,13,15,17] and Dbf4p is stabilized by inactivation of the APC in
Figure 1. The N-terminus of Dbf4p interacts with the Polo PBD. Cells co-transformed with various bait GAL4 DNA binding domain fusions
(GBD-DBF4) and prey GAL4 activation domain fusions (GAD-CDC5) constructs were spotted at 10-fold serial dilutions on SCM–Trp –Leu and SCM–Trp–
Leu–His plates containing 2 mM 3AT (A, C) or 0.5 mM 3AT (B) and incubated at 30uC for 2 to 3 days. The GBD-Dbf4 protein levels are shown in Figure
Dbf4p Inhibits Polo Kinase
PLoS Genetics | www.plosgenetics.org3 May 2009 | Volume 5 | Issue 5 | e1000498
Page 4
G1 or by removal of its N-terminal D-box [13,15–17]. Together
these data suggest that Dbf4p degradation can occur via both
APC-Cdc20p and APC-Cdh1p mediated ubiquitylation.
Since budding yeast Polo is not required for DNA replication
but promotes multiple mitotic activities, we reasoned that Cdc7p-
Dbf4p might influence Polo activity during mitosis. The dbf4-
ND109 mutant progresses normally through the cell cycle and does
not exhibit any obvious growth defects or temperature sensitivity
(Figure 3A, B) [26]. We therefore tested for genetic interactions
between dbf4-ND109 and the cdc5-1 temperature sensitive (ts)
mutant. At the restrictive temperature cdc5-1 cells arrest in late
telophase with divided nuclei, elongated spindles and high Cdk1p-
Clb2p levels indicating a failure to exit mitosis [34,35,62]. The
cdc5-1 mutant is ts at 30uC on rich media, but we found that dbf4-
ND109 suppressed the cdc5-1 temperature sensitivity up to 35uC
indicating a strong suppression of its growth defect (Figure 3A).
The dbf4-D82–88 mutant defective for interaction with Polo also
suppressed the cdc5-1 ts (Figure 3A). Since the cdc5-1 ts suppression
was reduced in heterozygous dbf4-ND109/DBF4 diploids com-
pared to dbf4-ND109/dbf4-ND109 diploids, i.e. dbf4-ND109 was
haploinsufficient (Figure 3C), our data strongly suggests that
Dbf4p is a Polo inhibitor and that loss of the Dbf4-Polo interaction
leads to increased cdc5-1 activity.
We confirmed that Dbf4p inhibited Polo activity using several
independent genetic tests. An extra plasmid copy of wild type
DBF4 but not dbf4-ND109 inhibited the growth of cdc5-1 cells
(Figure 3D). A dbf4-ND65 mutant that disrupts the D-box (residues
62–70) resulting in elevated protein levels was synthetically sick or
lethal in combination with cdc5-1 (Figure 3E). Since the dbf4-ND65
mutant exhibits a wild type growth rate and normal S-phase entry
and progression (data not shown; [26]) but binds to Polo, this
suggests that elevated Dbf4p levels are deleterious to cdc5-1
activity. Finally, elevated expression of the Dbf4p N-terminus from
the GAL1 promoter completely inhibited the growth of cdc5-1 cells
but had no effect on the growth of wild type (not shown) or a
mcm2-1 ts mutant. Mcm2 is a component of the MCM helicase,
which is thought to be the physiological target of Cdc7p-Dbf4p
during the initiation of DNA replication [11]. The inhibition of
cdc5-1 growth depended on the Dbf4p-Polo interaction, since
deletion of the PIR (residues 66–109) or Dbf4p residues 82–88
abrogated the growth inhibition (Figure 4A, B). Since the Dbf4p
N-terminus interacts with the PBD, this data suggest that
Figure 2. Dbf4p residues 67–109 interact directly with the Polo PBD. (A) HA-Cdc7p-Dbf4p kinase or HA-Cdc7p plus Dbf4p truncation
mutants were expressed in Sf9 cells together with Polo and immunoprecipitated using 12CA5 antibody. Blots were probed with anti-Myc (Cdc5p),
anti-Cdc7p and anti-Dbf4p polyclonal antibodies. (B) Endogenous HA3-Cdc7p-Dbf4p complexes were immunoprecipitated using 12CA5 antibody
from HA3-CDC7 DBF4 CDC5-Myc15 (M2741) and HA3-CDC7 dbf4-ND109 CDC5-Myc15 (M2743) yeast strains following nocodazole arrest and probed for
Cdc7, Dbf4, and Cdc5-Myc. (C) Purified Sumo and Sumo-Dbf467–109proteins were co-incubated with purified GST-PBD or GST alone; proteins were
pulled down using glutathione-Sepharose beads and blotted with antibodies against GST or Sumo. (D) In vitro phosphorylation of GST-PBD using
purified Cdc7p-Dbf4p kinase.
Dbf4p Inhibits Polo Kinase
PLoS Genetics | www.plosgenetics.org4 May 2009 | Volume 5 | Issue 5 | e1000498
Page 5
overexpression of Dbf4 N-terminal peptides interferes with
essential Polo-substrate interactions by competitive inhibition.
Together, these data indicate that the Dbf4p N-terminus inhibits
Polo activity and that this inhibition requires residues 66–109,
which are also required for the Dbf4p-Polo physical interaction.
We wanted to determine whether the Cdc7p kinase subunit is
required to inhibit Polo in the FEAR or MEN pathways. This is
not straightforward since Cdc7p is an essential protein kinase.
Importantly, inhibiting Cdc7p activity would not only inhibit
replication origin firing but would also likely induce the replication
checkpoint that inhibits the metaphase to anaphase transition and
MEN activation [4]. Inhibiting Cdc7p activity would thus interfere
with the mitotic pathways we would like to measure. Therefore,
we addressed this question indirectly by taking advantage of our
observation that high copy dbf4-ND109 suppressed the cdc5-1 ts
phenotype (Figure 4C). Since Dbf4p residues required for
interaction with Cdc7p map between residues 312–704 (C.G.
and M.W. unpublished data), Dbf4-ND109 protein (expressed in
high copy) will compete with full length Dbf4p (in single copy) for
Cdc7p binding. Therefore, our finding suggested that high copy
expression of Dbf4-ND109p reduced the cellular concentration of
wild type Cdc7p-Dbf4p, the likely Cdc5p inhibitor, which
suppressed the cdc5-1 ts allele (Fig 4C). High copy expression of
Dbf4-D82–88p that does not interact with Polo also suppressed the
cdc5-1 ts (Fig 4C). Importantly, deleting Dbf4p C-terminal residues
required for interaction with Cdc7p (CD312–704) eliminated the
ts suppression by high copy dbf4-ND109 and dbf4-D82–88. These
data are consistent with full-length Cdc7p-Dbf4p kinase acting as
the physiological Polo inhibitor.
Cdc7p-Dbf4p Phosphorylates the PBD
Wild type Cdc7p-Dbf4p might inhibit Polo abundance or kinase
activity during the cell cycle and thus explain our genetic data.
However, we saw little difference in Polo protein levels, cell cycle
expression or Polo kinase activity comparing wild type yeast with
the dbf4-ND109 mutant (Figure S2). This suggests that Dbf4p
inhibits Polo independently of altering its expression or kinase
activity. This is consistent with our genetic data since loss the
Dbf4p PIR suppresses the cdc5-1 allele yet the Cdc5-1 protein
retains considerable protein abundance and kinase activity at the
non-permissive temperature [63]. The mitotic exit defect associ-
ated with cdc5-1 is due to a single P511L amino acid substitution
preceding polo-box 1 of the PBD [64], strongly suggesting that the
cdc5-1 growth defect is caused by a defect in substrate recognition.
Since genetically DBF4 is a negative CDC5 regulator we
hypothesized that Cdc7p-Dbf4p phosphorylates Polo to prevent
Figure 3. The Dbf4p N-terminus inhibits Polo activity. (A) dbf4-ND109 rescues the cdc5-1 temperature sensitivity. Indicated strains were
spotted at ten-fold serial dilution on YPD and grown at increasing temperatures. (B) Cell cycle progression of wild type and dbf4-ND109 at 30uC by
flow cytometry of alpha-factor arrested (t=0) and released cells (t=10 to 180 mins). (C) The indicated diploid strains were spotted at increasing
temperatures (D) DBF4 cdc5-1 was transformed with the indicated ARS CEN plasmids and spotted at 25uC and 32uC (E) Representative tetrads from a
dbf4-ND65 (M2007) cross to cdc5-1 (M1614).
Dbf4p Inhibits Polo Kinase
PLoS Genetics | www.plosgenetics.org5May 2009 | Volume 5 | Issue 5 | e1000498


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There are 229 results - Show results on a map Print this page
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Describing itself as 'boutique style', this 17th-century former coaching inn is very much of the 21st century. The bedrooms, with Egyptian cotton linen and Molton Brown toiletries as standard, are eac...More about The Wensleydale Heifer
Within easy walking distance of Scarborough's beach and many other attractions, Howdale offers a comfortable lounge with TV, games console and lots of board games. All bedrooms have digital LCD TVs, a...More about Howdale
Well-equipped, spacious bedrooms are a feature of this Victorian gentleman's residence; one bedroom was originally the nursery. Close to the cliff lift which takes you down to the spa, beaches and gar...More about Olivers
Modern, budget hotel offering comfortable accommodation in bright and practical bedrooms. Breakfast is self-service and dinner is available in the restaurant. ...More about Ibis Budget Bradford
Located in Seamer, a village inland from Scarborough, this 18th-century guest accommodation contains comfortable, well-equipped en suite bedrooms. Breakfast is served at individual tables in the smart...More about North End Farm Country Guesthouse
This beautiful Victorian town house is conveniently located for the Yorkshire Dales and is only 20 minutes from the A1(M). Guests are welcome to use the attractive lounge, where there is an honesty ba...More about Homelands Guest House
York Pavilion Hotel is an attractive Georgian hotel situated in its own grounds. All the bedrooms are individually designed to a high specification; some are in the old house and some in the converted...More about BEST WESTERN York Pavilion Hotel
This country pub and lodge is convenient for Harrogate, with its tourist attractions, shops and tearooms as well as the Conference Centre. It is also well located for visiting picturesque Knaresboroug...More about Innkeeper's Lodge Harrogate (East)
This Innkeeper's Lodge is a 19th-century Victorian property idyllically located in the Yorkshire Dales, with stunning views over Wharfedale. Perfect for walkers, browsing the spa town of Ilkley, close...More about Innkeeper's Lodge Ilkley
The Grainary is a delightful property set in acres of its own land with animal enclosures and duck ponds - very appealing for children and families. The tea room has stunning views over the countrysid...More about The Grainary


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Bavarian Lodge Ski & Tennis Resort
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
TripAdvisor Reviews Bavarian Lodge Ski & Tennis Resort Big Bear Lake
Travel Blogs from Big Bear Lake
Mountains and Lakes...
... on up. Thomas and Ryan especially were in their element climbing up rocks, jumping down scree slopes. Kyle was punching well above his weight for a 3 year old and Isabel was worryingly following Thomas everywhere he went. The trail plateaued off with a great vista on to Big Bear lake and we soon turned around and headed back
The boys and Isabel ran off down the trail and met with the ladies party back at the car and after a bit of ...
Papa Smurf and Mountain Mama
... an unworldly amount of luck can one hope to lay eyes on the Northern Terminus. We all pooled money back at Papas and ordered 10 pizzas from Dominos so Josh didn't have to spend his evening making us dinner while Papa and Mama were out working their second jobs. These are definitively salt of the earth type folks and through and through loving Christians. People like this implore to always strive to be the best I can and to maximize my potential and capacity for ...
Almost Went Over The Fisical (sic) Cliff!
... of the desert floor. They look like man made creations found in Walt Disney World or Disneyland.
Now for an explanation of todays title- "Almost Went Over The Fisical (sic) Cliff":
Fisical, Physical, get it?
We arrive at Keys View and park the truck. We didn't just get out of the truck- we were pulled out! The wind caught our doors like sails and we literally hit the pavement running. The desert floor is nearly 4000 feet below us. Mt. Jacinto ...
How Aboot The Weather, Eh?/ Off To La Quinta
... on long loop streets with each loop connected to the main access road.
We have walked all of these loops a few times now and this is what we have observed:
1. Many, many license plates from British Columbia, Saskatchewan, Nova Scotia, and Alberta Canada. How aboot that, eh?
2. A lot of people that would spell the word "favorite" as "favourite", "color" as "colour"
3. Many people that pronounce "about" as "aboot" and end almost ...
California Dreaming
... it is fairly accurate and it feels quite good so we go with it. He has taken to calling us Aunty Bree and Uncle Noah – so we feel 'Crew' is a step forwards.
We wander around the shop aimlessly before picking up salad, cheese, bread for today and tomorrow as well as snacks and drinks.
We make it home and have the most lovely dinner. Elwood was so chatty and was telling us the least funny jokes I have ever heard. ...


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Green Initiatives: Cost, Achieving ROI Challenge Hotels
The American Hotel & Lodging Association's Green Assessment Survey results reveal the top 10 green initiatives hotels are enacting, the top 10 green programs hotels can improve upon, motivations and challenges for implementing green lodging practices, and quantifiable measurements of green action being taken around the nation.
The biggest challenges or barriers hoteliers face in implementing green lodging practices:
1. Having enough capital to invest
2. Achieving ROI
3. Ability to find vendors/suppliers offering the products they need
4. Having enough time
5. Limited knowledge/resources
6. Garnering support from government or local organizations
7. Possibility of negative guest reactions
The biggest motivations for implementing green lodging practices:
1. Environmental benefits and building guest loyalty (tie)
2. Financial savings
3. Increasing market share
4. New revenue opportunities
5. Marketing and public relations coverage
6. Preempting government regulations
The top 10 green things hotels are doing well:
1. Offering linen reuse program
2. Recycling paper
3. Using compact fluorescents in guestrooms
4. Using compact fluorescents in public spaces
5. Offering towel reuse program
6. Training maintenance staff on conservation and energy procedures
7. Recycling cardboard
8. Water conserving retrofits for showers
9. Recycling program in administrative areas
10. Tracking energy and water consumption on a monthly basis
The top 10 green things hotels can improve on:
1. Having occupancy sensor controls for the thermostat in guestrooms
2. Recycling food waste
3. Using indoor paints that are low or zero VOC (volatile organic compounds) or Green
Seal Certified
4. Using T8 lamps in corridors
5. Giving preferential treatment to vendors/suppliers who offer recycled products
6. Using daily cleaning chemicals that have a MSDS health rating of "1" or less and/or
Green Seal Certified
7. T8 lamps in back of house
8. Tracking generation of solid waste on a monthly basis
9. Recycling program in guest rooms
10. Recycling program in recreational areas
Green Assessment Survey results were compiled based on 217 responses from AH&LA member hotels.
Stay Up-to-Date On Environmental Management, Energy & Sustainability News with EL's Free Daily Newsletter
Reader Comments
hotels are good candidates for small scale combined heat and power applications. Capstone 65kW unit low cost of ownership fast payback.
Energy Manager News
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- New York City Continues to Blaze Building Efficiency Trail with Launch of Its Data-Driven NYC Retrofit Accelerator
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- Six Steps to a Winning GRESB Audit
- Mexico Shopping for 2M Smart Meters
- Raleigh, N.C. Finally Getting its LEDs
- Wireless Can Make for a More Energy-Efficient BAS System


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A quote from the C++ Primer, 4th edition:
The rules for when an exception matches a catch exception specifier are much more restrictive than the rules used for matching arguments with parameter types. Most conversions are not allowedthe types of the exception and the catch specifier must match exactly with only a few possible differences:
1.Conversions from nonconst to const are allowed. That is, a throw of a nonconst object can match a catch specified to take a const reference.
Could you give me an example to explain the first term? Thanks a lot!


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Anti Essays offers free essay examples to help students with their essay writing.
Below is a free essay on "Social Class Vs Health Inequalities" from Anti Essays, your source for free research papers, essays, and term paper examples.
"Social Class Vs Health Inequalities". Anti Essays. 13 Oct. 2015
Social Class Vs Health Inequalities. Anti Essays. Retrieved October 13, 2015, from the World Wide Web: http://www.antiessays.com/free-essays/Social-Class-Vs-Health-Inequalities-82270.html


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CU recognized for success of its Center of Excellence for Watershed Management
Authorities have approved Clemson University's Center of Excellence for Watershed Management to keep operating for five more years.
Representatives from the U.S. Environmental Protection Agency, the South Carolina Department of Health and Environmental Control and Clemson University recently signed a memorandum of understanding renewing the center.
The renewal was the first of its kind among the 10 centers across the Southeast, Clemson officials said.
To become a recognized Center of Excellence, an institution must demonstrate: technical expertise in identifying and addressing watershed needs; involvement of students, staff and faculty in watershed planning, protection, and restoration; capability to involve the full suite of disciplines needed for all aspects of watershed management; financial ability to become self-sustaining; ability to deliver and account for results; willingness to partner with other institutions; and support from the highest levels of the organization.
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Important delivery notice
The products on this site are only delivered to UK addresses. If you require delivery to another country please visit one of our other sites below.
Clematis plants, commonly known as 'Old Man's Beard', have a multitude of uses in the garden. Large flowered clematis will quickly transform walls and fences during the summer. Grow evergreen clematis and winter flowering clematis varieties for colour and interest during the darkest months, or hide ugly outbuildings with the vigorous growth of Clematis montana. You can even find dwarf clematis for smaller gardens or trailing from patio pots.
View our full range of clematis plants for sale below. Find out more about growing clematis in our 'Climbing plants for walls and fences' article and how to prune a clematis.
Clematis 'Royal Ladies Collection'
Old Man's Beard
These three beautiful climbers feature a profusion of tulip-shaped buds that open out into magnificent, bell-shaped flowers as the flowers mature. Grow Clematis 'Royal Ladies Collection' against walls, fences, trellis, or in large containers on the patio and enjoy a long season of interest. These Clematis plants are particularly easy to maintain as they can be cut back to almost ground level each year. Height: 3m (10'). Spread: 1.5m (5') Pruning group: 3.
This collection consists of one plant of each of the following varieties:
Clematis 'Princess Kate'
Clematis 'Princess Diana'
Clematis 'Queen Mother'
Useful links:More info
Clematis 'Mayleen' (Large Plant)
Clematis montana, Old Man's BeardIt could almost be known as the 'mile-a-minute' clematis, such is the rapid growth of this variety. Clematis 'Mayleen' is often considered the best scented montana cultivar, with delightful satin-pink ruffled blooms and attractive bronze foliage. This classic Clematis is perfect for smothering walls, fences, pergolas and sheds in masses of late spring blooms. Height: 10m (33'). Spread: 3m (10'). Pruning group: 1. More info
Clematis 'Comtesse de Bouchaud' (Large Plant)
Old Man's BeardA vigorous and beautiful late-flowering clematis which holds a coveted RHS AGM. Clematis 'Comtesse de Bouchard' dates back to 1900, but still holds its own among modern cultivars. Its mauve-pink flowers appear from June to September in a spectacular display. This tough, forgiving climber is particularly versatile, performing superbly in borders or containers in almost any aspect of sun or shade. Height: 3m (10'). Spread: 1m (3'). Pruning Group: 3. More info
Clematis 'Dr Ruppel' (Large Plant)
Old Man's BeardMagnificent flowers are produced in abundance in early summer with a rose-pink blush along the length of each petal. A second flush appears in late summer, followed by pretty spherical seed heads that add garden interest well into the autumn. Clematis 'Dr. Ruppel' is a compact cultivar that is excellent for containers or adding a bright splash of colour to a sunny wall. Height: 2.5m (8'). Spread: 1m (3') Pruning group: 2. More info
Clematis 'Remembrance' (Large Plant)
Old Man's BeardClematis 'Remembrance' bears raspberry pink flowers that later mature to a very deep pink with a central boss of pale yellow stamens. The sepals are heavily textured giving each bloom a satiny sheen. This compact late flowering Clematis blooms consistently from July to September. A pretty variety which makes a lovely feature in a large patio pot and trained onto trellis or a free standing obelisk. It's easy to maintain too - simply cut the stems back to almost ground level each year. Height: 2.5m (8'). Spread: 1m (3'). Pruning group: 3. More info
Clematis montana 'Continuity'
Old Man's Beard
Useful links:More info
Clematis (Top to Bottom) 'Success Magenta'
Old Man's BeardThis stunning, large-flowered Clematis has been specially selected for its ability to flower from its base to its tips. Vibrant, magenta blooms with contrasting creamy coloured stamens cover Clematis (Top to Bottom) 'Success Magenta' throughout summer for a fabulous, long-lasting display. This free flowering variety also has a neat, compact growth habit making it ideal for growing in large containers on the patio. Height: 2.5m (8'). Spread: 1.5m (5'). Pruning group: 2.
Useful links:More info
Clematis (Top to Bottom) 'Success Salmon'
Old Man's Beard
Useful links:More info
Clematis 'Dancing Smile'
Old Man's Beard, Clematis 'Zodasmi'Absolutely unique! This stunning climber boasts amazing double flowers - just like a dahlia. Clematis 'Dancing Smile' was developed from cross-breeding in Germany in 2004. The spectacular display in May and June is followed by another flush of blooms later in the summer. Supplied as a pinched plant with 3 breaks. Height: 150cm (60"). Spread: 75cm (30"). Pruning group: 2.
Useful links:More info
Clematis montana 'Sunrise'
Old Man's BeardBronze foliage compliments the fully double pink blooms of Clematis montana 'Sunrise'. This eye catching variety will quickly form a wall of colour, romping freely to cover up unsightly walls and fences or soften the edges of outbuildings and garden sheds. Unlike many montanas, this double flowered Clematis is less vigorous and easier to contain within its boundaries. Height: 4m (13'). Spread: 2m (6',5"). Pruning group: 1
Useful links:More info
Clematis 'Tae'
Old Man's Beard
Clematis 'Alionushka' (Large Plant)
Clematis integrifolia, Old Man's BeardClematis 'Alionushka' is a semi-herbacous plant with non clinging stems and a loose, sprawling habit. This free flowering variety bears an abundance of small bell-shaped, mauve-pink flowers from early summer right through to September. This superb Clematis integrifolia hybrid makes a lovely feature scrambling among borders where it can use mature shrubs for support, or let it cascade over banks and low walls. Height: 2m (6'). Spread: 1m (3') Pruning group: 3. More info


text:
Walberton News and Events
The Parish Office will be closed from the 12th to the 16th October. In an emergency please contact the Chairman on 01243 555062.
Lock it or lose it
04 September 2015
Burglaries in Barnham Road and Fontwell Avenue on the 3rd September 2015. Can you help?
Could you be a Councillor?
14 August 2015
We have vacancies for Parish Councillors. Could you carry out this important community role?
Walberton Neighbourhood Plan Regulation 14 Consultation
10 August 2015
Public and statutory bodies consultation under Regulation 14 of the Neighbourhood Planning (General) Regulations 2012. Have your say on your Plan.
Concerned about speeding?
10 August 2015
Speeding continues to be a concern for many rural communities. If you feel that speeding in our area is serious enough to warrant your involvement – you could help!
Planning Matters
03 August 2015
Did you know that we publish details of planning applications affecting Walberton as soon as they are registered by Arun District Council?
What's on in Walberton
Village Market and Traditional Tea Room in the Pavillion
Saturdays from 1000 to 1300
For details on current and future events please see our Events Pages
If you would like to add an event or tell us about one please go to this page
Find out What's On at Walberton Village Hall


text:
Non-Metallic Biomaterials for Tooth Repair and Replacement
Edited by
- P Vallittu, University of Turku, Finland
Part one reviews the structure, modification and repair of dental tissues. The properties of enamel and dentin and their role in adhesive dental restoration are discussed, along with biomineralization and biomimicry of tooth enamel, and enamel matrix proteins (EMPs) for periodontal regeneration. Part two goes on to discuss the processing, bonding and wear properties of dental ceramics, glasses and sol-gel derived bioactive glass ceramics for tooth repair and replacement. Dental composites for tooth repair and replacement are then the focus of part three, including composite adhesive and antibacterial restorative materials for dental applications. The effects of particulate filler systems on the properties and performance of dental polymer composites are considered, along with composite based oral implants, fibre reinforced composites (FRCs) as dental materials and luting cements for dental applications.
With its distinguished editor and international team of expert contributors, Non-metallic biomaterials for tooth repair and replacement provides a clear overview for all those involved in the development and application of these materials, including academic researchers, materials scientists and dental clinicians.
Students and academics, such as dental materials researchers at dental schools; General dental practitioners and specialists; Those interested in biomaterials applications
Book information
- Published: December 2012
- Imprint: Woodhead Publishing
"...the contributors of this excellent piece of literature have been able to create a clear overview on the use of "non-metallic" materials for tooth restoration and tooth replacement."--
The reader is made mindful of the importance of acknowledging progress in the field and the value in responding to advances in the theory underpinning the method of tooth repair and replacements., P. Raphy, British Dental Journal
The book offers an objective look, in a concise manner and with extensive references, at the dental use of "non-metallic" biomaterials for dental restoration and tooth replacement based upon evidence from publications in only 3 general parts., M Tabak, Biomaterials Network


text:
News - Page 8
2014 NBA Finals Preview — Miami Heat vs. San Antonio Spurs
Army Sergeant Bowe Bergdahl Released From Five-Year Captivity in Afghanistan
On Saturday, May 31, 2014, Americans learned that Idaho native Army Sergeant Bowe Bergdahl had been released in Afghanistan after being held prisoner for nearly five years. He was exchanged for prisoners in Guantanamo Bay, who will now be sent to Qatar.
"On behalf of the American people I was honored to call his parents to express our joy that they can expect his safe return," President Obama said in a statement distributed by the White House.
2014 NFL Draft Recap: Winners, Losers, Steals & Reaches
Hilarious Video Shows Racism in Sports Is A-Okay [VIDEO]
If Your Family Was Homeless, Would You Recognize Them? [VIDEO]
A new social experiment from the New York Rescue Mission asks the question, 'If your family was homeless on the streets of New York City would you even recognize them?'
[UPDATE] Hundreds Feared Dead After Ferry Sinks In South Korea
Live Stream For Tonight's First Total Lunar Eclipse of the Year
It's the first total lunar eclipse of the year. It can be seen only in North and South American, however NASA will also stream the eclipse live.


text:
(765) 447-0200
Monday - Friday
9:30 am - 5:31pm
9:30 am - 4:31pm
Summer Sterling Silver Sell Off Sale!
This week only we are having our Summer Sterling Silver Sell Off Sale! Thousands of pieces of jewelry starting at just $3!!!! Rings, Pendants, Necklaces, Earrings, Bracelets, Modern, Designer, Antique, Vintage, Estate & MORE! August 24th through August 29th!!!
Our goal with this web site is to be informative, but not in a dull and boring way, you can find that anywhere! We are going to try and keep this site fresh by frequently updating the content with new and interesting products, recent acquisitions of cool and significant items, and special offers only posted here. We want you to keep coming back and realize that to do this, we have to give you a reason!
At Vierk's Fine Jewelry you will discover that we are not your traditional Jewelry Store…we think we are much more. And we also hope you will not find our web site traditional. We like to showcase one-of-a-kind items, we like to use fun words (like intuitive, and effervescent), and we like to keep our clients, customers, and friends smiling.
Now that you're here, please take the time to tour the site. You will find some of that basic "nuts and bolts" information but hopefully you will find something new, interesting, and entertaining also. Contact points should be easy to find throughout the site and we sincerely hope that you will get in touch with us to answer any questions, help you in any way, or just to say hello and give us your feedback.
At Vierk's Fine Jewelry, we will leap tall buildings in single bounds, pull rabbits from hats, and generally bend over backwards to become your "Personal Jeweler".
Jeff Vierk
Copyright (c) Vierk's Fine Jewelry | 1650 Main Street Lafayette, IN 47904 | 1-800-695-2938 | (765) 447-0200Website by Dunsen Design


text:
Photo Galleries
< MyNorthwest
Week in photos
Local little slide on home, Gatorade shower, the best of tango
43 of 44
In this photo taken late Sunday, Aug. 18, 2013, Egyptian citizens search cars at a makeshift checkpoint on Corniche El-Nile Street, Maadi, Cairo, Egypt, late Sunday, Aug. 18, 2013. Egypt's military leader vows that the army will not tolerate further political violence after nationwide clashes leave hundreds dead. (AP Photo/Amr Nabil)
Photo by Amr Nabil
Photo by Amr Nabil
43 of 44
More Photo Galleries
Week in photos
Photos taken by the Associated Press Sept. 28 to Oct. 2.
Stargazers treated to supermoon eclipse
Stargazers were treated to a rare astronomical phenomenon with a total lunar eclipse combined with a so-called supermoon.
Week in photos
Photos from the Associated Press taken from around the world.
Four killed in Aurora Avenue Bridge crash in Seattle
The four people killed when a charter bus and Ride the Ducks vehicle collided were students from North Seattle College, The Seattle Times reports.
Big-name business leaders meet China's president in Seattle
Business leaders from Apple, Honeywell, Disney, Microsoft, Starbucks, Dow Chemical, Amazon, IBM, and many more attended a Wednesday morning meeting with the president of China.
Week in photos
Photos taken from around the world during Sept. 14-18.
Week in photos
Check out the week in photos from all over the world.


text:
By: KillPhil
A commercial for the new Ratchet & Clank game on the PSP, featuring the Shrink Ray.
Views: 4,376 | Posted: 05/25/2011
By: salamone
A new look at the next Ratchet instalment.
Views: 3,581 | Posted: 05/25/2011
By: greenergie
Ratchet and Clank Japan ad for PS2
Views: 703 | Posted: 05/25/2011
By: Pezman
The second Ratchet and Clank Future Trailer. It's awesome!
Views: 977 | Posted: 03/14/2011
By: KillPhil
A commercial for the new Ratchet & Clank game on the PSP, featuring the Mootater. [url=[make_url]arc:content:gt-authoring:82ba8350-8a9c-4e41-8a23-83b7eab6aba0[/make_url]]The other Ratchet & Clank: Size Matters commercial[/url].
Views: 1,685 | Posted: 10/16/2008
By: KrazieCanuck
Video interview performed by Shane Bettenhausen and Brian Intihar of EGM discussing the new Ratchet & Clank PS3 game trailer and providing more feedback regarding the game. EGM = Electronic Gaming Monthly Shane Bettenhausen - Excutive Editor Brian Intihar - Previews Editor
Views: 6,867 | Posted: 08/09/2008
"Time for some real action"
Views: 93,810 | Posted: 10/17/2005
Ratchet taking the landstalker for a spin.
Views: 18,263 | Posted: 08/26/2005
Interview with Brian Hastings, Chief Creative Officer.
Views: 11,245 | Posted: 08/18/2005
You're the next contestant on Dreadzone!
Views: 50,732 | Posted: 05/23/2005
Week of
Oct. 04
Week of
Oct. 11
Release Date: 10/06/2015
A non-linear episodic game with 6 acts.
PS4, Xbox One, PC
Release Date: 10/07/2015
The 2015 edition is revealed.
Release Date: 10/09/2015
The Uncharted series is back and remastered for PlayStation 4.
PS4, Xbox One, PC, Xbox 360, PS3, 3DS
Release Date: 10/13/2015
The monsters of R.L. Stine's popular book series are back to haunt in game form.
PS4, Xbox One, PC
Release Date: 10/13/2015
Xbox One, PS4, PC
Release Date: 10/13/2015


text:
46Re: 346 xt availability
Expand Messages
- May 6, 2009--- In email@example.com, "celticirishkeith" <celticirishkeith@...> wrote:
>I would just buy online elsewhere for less money.
> hi all ,
> anyone know if either radioshack or bestbuy will be selling
> the 346xt. i checked on their web sites and they have alot of scanners
> just not this one.
> keith
- << Previous post in topic Next post in topic >>


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EyeCare of Longwood
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Can Excess Fat Be Taken from the Vagina?
Doctor Answers 5
Yes fat reduction from the vaginal area can easily be done using liposuction.
Excess fat in vaginal area.
The area surrounding the vagina or mons pubis as it is known often enlarges with weight gain and is a concern to women. This can be easily addressed with liposuction or excisional surgery under local anesthesia.
Web reference: http://www.drvitenas.com/vaginoplasty.html
Liposuction of the pubic area
You are most likely referring to the "mons pubis" and yes, you can have the excess fat removed through Liposuction from that area.
Thank you for your question.
Web reference: http://sfcosmeticsurgery.com/photos/labioplasty/images/liposuction_public_area.jpg
You might also like...
Fat accumulations in the external genitalia (vagina, labia, mons)
The vagina does not accumulate fat. However, of the external genitalia, fat can be localized to the mons pubis or labia majora. I have attached a video which describes the anatomy.
Web reference: http://www.bodysculptor.com/labiaplasty.html
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


text:
UK Goverment demands YouTube remove videos
Google has recently released its bi-annual Transparency Report which states that there have been 135 requests from the government and police to remove videos which may cause a threat to national security.
The 135 requests were made from the beginning of 2011 to the end of June, and is a 100 per cent increase on last year where no video take-down requests were filed.
This increase constitutes somewhat of an online video crackdown when violence is seen to be incited through YouTube.
However, whatever your views on freedom of speech and what should or shouldn't be allowed to be aired, this kind of censorship appears to be on the increase.
Fraudulent adverts have also been the target of the Office of Fair Trading, up from 156 to 333, whilst the Transparency Report also revealed that authorities were attempting to access more user identities than before. From 1,162 requests in the previous year, 1,273 were made in the following 6-month period in 2011.
Another figure to come out was that the British internet-using public, relative to population size, is most likely to have its data requested by the Government. Google states that it complied with Government requests 82 per cent of the time.
This is a huge moral maze in terms of freedom of speech and the extent to which certain views, if made public, have the ability to cause harm to others. There is much on the Internet that is unpleasant, and there often seems to be somewhat of a grey area of what is okay to be on public view and what isn't.
Do you think the UK Government is right to exercise censorship over videos it deems unacceptable or should there be no limit to what people can say online? Let us know in the comments...
Master HTML5 and CSS3 web design for 81 per cent off
Best TV and movies to watch this weekend on Netflix, NOW TV and more: Doctor Who, San Andreas, Scream...
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- Samsung Galaxy View is an iPad Pro rival with a crazy 18.5-inch screen, amazing leaked specs reveal all
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- Siri, turn off my lights: Philips Hue with Hue Bridge 2.0 tested
- Microsoft Surface Pro 4: Release date, rumours and everything you need to know
- Best smartphones to look forward to in 2015
- Forza Motorsport 6 review: The Forza is strong with this one
- Hanging around with Nathan Drake: Pocket-lint chats to Nolan North about gaming superstardom
- What is Freeview Play, when is it coming and how can I get it?
- Can't afford the Xbox One Elite controller? Don't worry, you'll be able to remap your existing controller buttons


text:
North Andover Booster Club Basketball Boys: Welcome
Online Registration for Basketball will open on 8/1!! Please choose the correct age group for your child when registering and register early to avoid late fees!!
Welcome to the North Andover Booster Club Boys Basketball program. The North Andover Booster Club offers basketball programs for boys in grades 2-8.
If you have questions, please email Co-Commissioners Ali Mandalinci (for in-town programs) or Dan Fay (for travel programs).
Bookmark this page and visit the website for information and schedules throughout the season!
Goal: To create a fun and safe environment to introduce the fundamental skills of basketball to the youngest players.
Format: Clinics on Saturday mornings for approximately 60 minutes from early December to late February.
The cost of the program is $40. Registration will open from Augusts 1st through November 1st. Late registrations will close by November 15th and will be available only if space permits.
Goal: To create a fun, safe, and exciting activity. To expand fundamental basketball skills to developing players. To introduce the basic concepts of team play. To encourage good sportmanship both on and off the court.
Format: Players will be assigned to teams. Teams will have one practice during the week, and one game per week on Saturday mornings at the North Andover Youth Center. Teams will play in a league format with an eight game schedule followed by playoffs.
The cost of the program is $80. Registration is open from August 1st through October 15th. Note: Late registrations will close by November 15th and will be available only if space permits.
Goal: To create a safe, exciting activity at different competitive levels for older players to continue development of individual basketball skills. To focus on team concepts. To support good sportsmanship on and off the court.
IN-TOWN LEAGUE - Approximately 12 -14 teams compete in Gr. 5-6 and Gr. 7-8 divisions. Teams will have at least one practice and one game per week. Practices and games run from early December thru late February/early March.
The registration fee is $110 and is open from August 1st through October 15th. An additional fee of $20 will be charged for registrations after this date. Note: Late registrations will close by November 15th and will be available only if space permits.
TRAVEL LEAGUE - The travel program is designed for players with more advanced skills, and requires a greater time commitment than the in-town program. Players MUST be North Andover residents (league rule) and are chosen through a try-out process. Please check this site frequently for the try-out schedule.
Every effort will be made to have two teams per grade. Teams will compete in the Merrimack Valley Youth Basketball league. Teams have two practices and up to two games per week. Travel teams may also participate in three competitive tournaments during the season.
Information including game schedules and directions to gym locations can be found on the leagues' website Merrimack Valley Youth Basketball.
Besides the registration fee of $110, an additional fee of $80 is collected by the coaches AFTER a player has made a travel team.
Travel Team tryouts will be posted to this website once scheduled. You must register before tryouts.
Thursday, April 28
Learn how to become a Friend of the NABC. Individuals or Businesses can support the NABC by placing a banner on this site. A great way for businesses to advertise!
Handout: Friends of NABC


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Ashley Greene gets a passionate kiss from her boyfriend Paul Khoury at the stroke of midnight at the New Year's Eve 2014 celebration held at Beacher's Madhouse on Tuesday (December 31) at the MGM Grand Hotel & Casino in Las Vegas.
Also in attendance at the event were Michael B. Jordan, Ryan Lochte, Lance Bass and fiance Michael Turchin, Derek Hough, and Mario Lopez with wife Courtney.
"What a fun night w the best New Years crew!!" Lance wrote on Instagram along with a photo of a bunch of the group.
FYI: Michael is wearing head-to-toe Gucci.
10+ pictures inside of Ashley Greene and Michael B. Jordan at Beacher's Madhouse… More Here! »


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I like my life somewhat private. with my friends my life is an open book. I'm a recovering alcoholic so I do not drink and do not use drugs. I have an awesome 12 year old son that I have raised on my own.
Join OkCupid
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43 • Sidney, NY • Man
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Pope Benedict XVI On the Easter Triduum
"Love Is Stronger Than Hate, It Has Triumphed"
VATICAN CITY, MARCH 22, 2008 (Zenit) - Here is a translation of the greetings Benedict XVI gave today to students participating in the international UNIV congress who had gathered at St. Peter's Basilica, and the catechesis he gave afterward during his weekly general audience in Paul VI Hall.
* * *
[Pope's greeting to students in St. Peter's Basilica]
[In English, he said]
Dear Friends,
I offer a cordial welcome to all of you who have come to Rome from various countries and universities to celebrate Holy Week together, and to take part in the International UNIV Congress. In this way, you will be able to benefit from moments of common prayer, cultural enrichment and a helpful exchange of the experiences gained from your association with the centres and activities of Christian formation sponsored by the Prelature of Opus Dei in your respective cities and nations.
[In Spanish, he said]
You know that with a serious personal commitment, inspired by the Gospel values, it is possible to respond adequately to the great questions of our time.
The Christian knows that there is an inseparable link between the truth, ethics and responsibility. Every authentic cultural expression contributes to form the conscience and encourage the person to better himself with the end of bettering society. In this way one feels responsible before the truth, at the service of which one must put one's own personal liberty.
This certainly has to do with a mission requiring commitment, and to fulfill it the Christian is called to follow Jesus, cultivating an intense friendship with him through prayer and contemplation.
To be friends of Christ, and to give testimony of him wherever we are, demands, furthermore, the strength to go against the grain, remembering the words of the Lord: You are in the world but not of the world (cf. John 15:19)
Do not be afraid, then, to be nonconformists when it is necessary; at your university, school and in all places.
[In Italian, he said]
Dear young people of UNIV, be leaven of hope in the world that desires to meet Jesus, often without knowing it. To better the world, make an effort above all to change yourselves through an intense sacramental life, especially through approaching the sacrament of penance, and participating assiduously in the celebration of the Eucharist.
I commend each one of you and your families to Mary, who never stopped contemplating the face of her son Jesus. I invoke over each one of you the protection of Saint Josemar�a and of all the saints of your lands, while I heartily wish you a happy Easter.
[Catechesis in Paul VI Hall]
Dear brothers and sisters
We have reached the eve of the Easter triduum. The next three days are commonly known as 'holy' because they allow us to relive the event central to our Redemption. They lead us to the nucleus of Christian faith: the passion, death and resurrection of Jesus Christ. These three days could be considered one single day. They make up the heart and are the key to both the liturgical year and the life of the Church. At the end of Lent we also enter that climate which Christ himself experienced back then in Jerusalem.
We want to rekindle in ourselves the living memory of the suffering which our Lord endured for us and to joyously prepare ourselves for next Sunday �"the true Passover, which the Blood of Christ has covered with glory, the Passover on which the Church celebrates the Feast that is the origin of all feasts� as stated in the preface for Easter in the Ambrosian rite.
Tomorrow, Holy Thursday, the Church remembers the Last Supper during which our Lord, on the eve of his own passion and death, institutes the sacrament of the Eucharist and that of ministerial priesthood. On that same evening, Jesus gave us a new commandment, "mandatum novum," the commandment of brotherly love.
Tomorrow morning, before entering the Easter triduum, but very closely tied to it, the "Messa Crismale" will take place in every diocese during which the bishop and priests of the diocese renew their promises made at ordination.
Also, the oils used to celebrate the sacraments are blessed: the oil for the catechumen, the oil for the sick and the holy chrism. It is one of the most important moments in the life of every Christian diocese, which, gathered around it's pastor, strengthens it's unity and faith in Christ, the supreme and eternal priest.
In the evening during the "Cena Domini" Mass, we remember the Last Supper when Christ gave himself to all of us as the food of salvation, as the drug of immortality and the mystery of the Eucharist -- source and pinnacle of Christian life.
Through this sacrament of salvation the Lord offered and realized for all those who ...
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1 - 2 of 2 Comments
7 years ago
I cannot seek to be a priest because I am ex-Anglican (and married), although pray that the Church to which I used to belong may come to accept that abortion is so anti-God (and not a matter for human choice politics).
I also pray that the Church of England may seek more earnestly to belong to the Universal and Catholic Church.
7 years ago
I will like to be a priest to save my soul and that of others.
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