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Buckhorn Inn
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How has this b&b rated in the past?
- Continental Breakfast
- Suites
- Restaurant
- Wireless internet connection in room (free)
- Refrigerator in room
- Room service
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Historical Traveler Reviews Buckhorn Inn Gatlinburg
My husband and I stayed at the Buckhorn Inn for our honeymoon on Nov. 13-15. We were completely satisfied with our choice of staying there. We were upgraded to a cottage for free, which was very nice and VERY private! The breakfasts were absolutely fantastic every morning, and the staff were all very nice as well. We loved that it was far enough away from Gatlinburg and Pigeon Forge, but still close to the park. We would go back in a heartbeat!
Just back from this serene and hospitable inn. If your looking for a get-away to relax, enjoy nature and reconnect with loved ones, this is the place. The views are majestic, the area so peaceful, the cuisine delectable and the room are so romantic. Especially the ones with king beds, gas fireplaces, and whirlpools. The decor is perfect and the rooms are immaculate. We will return as often as we can.
So Lovely and Peaceful
My family loves the Smokies, so we have often traveled to Gatlinburg. This inn, however, makes you feel like you are the guest of honor on a charming country estate in England or Ireland. Beautiful rooms, lovely antiques, fluffy towels, highly quality sheets, and a gracious host and hostess to leave a key and a cheery note upon your late arrival, and to greet you at breakfast the next morning. There was a gloomy spring rain the entire time we were there, but we still felt warm and snug at the Buckhorn. I look forward to staying there again in the future.
Makes Memories
3 Days in a luxurious cabin with every amenity made memories - great view into the nature and up to mountains. fireplace (not needed in the 90�), kitchen, bathroom all very tasteful, clean and the quietest location next to all attractions, porch and patio, their nature trail took us in about 45 minutes through their grounds with pond, birdwatching ,wildlife,acces to a art gallery on neigboured grounds,
The daily four cours dinner at $ 30 is a real chiefs work, we loved it and were unhappy to miss it one evening because we did not book one day in advance, they were complete days before! it was the highlight of 3 weeks USA.
Its family owned and operated, what you feel in every thing and by every staff member you'll meet.
Exceeded all expectations
This inn is a gem. We stayed here for four nights in June. The hotel is furnished traditionally and to a high standard. Our suite was large and comfortable with a lovely view and a verandah. The atmosphere was welcoming and tranquil. Everything we needed was at hand and staff were attentive without being overbearing. There was a wide choice at breakfast and the food was excellent. We had dinner in the inn on 3 nights which varied from good to excellent. The dining room has superb views of the mountains which made our meals even more enjoyable. We would highly recommend this inn and wish it was nearer so that we could go for a weekend break. Our stay at the inn was extremely relaxing and exceptional value.
Hidden Treasure!
The Buckhorn Inn, a lovely spot, shares an address with Gatlinburg, but thankfully, that is about all! Far from the crowds and commercialism, the Inn is a reminder of what makes this area such an attractive place to vacation. We stayed in a "cottage" which turned out to be a well-built, private accomodation, with screened in porch, living room with fireplace, small but functional kitchenette, nice sized bedroom and luxury bath with huge jacuzzi tub. I'm a tough customer and I was extremely pleased. The setting for the Inn is beautiful and peaceful, the grounds are a wonderful combination of groomed and wild, and the buildings are impeccably maintained. We ate dinner there one night and it was excellent, as were the breakfasts. I'd describe the food as hearty American--rich, well prepared and generous in portion. Everyone was very friendly and helpful. The rooms were reasonbly priced and a very good value for what you got. A great place!!
Great weekend!
We spent 3 nights at the Buckhorn Inn and did not want to ever leave! We stayed in the Webb Mountain House which was wonderful. Very private with beautiful views from every window. Breakfast was delicious and the staff was wonderful. We hope to return every year!
Memorable Experience
The Inn was amazing. We had a memorable experience. We got engaged on the trip. The staff was very helpful. I think they have thought of every little detail. The breakfasts were wonderful and relaxing looking out the windows. We also ate dinner one night at the Inn and you must do so to funnly appreciate Buckhorn Inn. It was very cozy and romantic. I would definately recommend this to all couples. Next time we go we are going to take family and friends with us. They have set the bar very high for all B & B's.
A place set apart . . .
Buckhorn Inn is a 'place apart' -- apart from the over-crowded and commercial Gatlingburg-area, apart from the 'modernity'of so many establishments, and apart from the 'sameness' that has come to characterize many inns in the U.S.
Buckhorn Inn occupies vast acreage outside of Gatlinburg, Tennessee, in a tranquil setting, with beautiful views of the Great Smoky Mountains.
Although the Inn is small/medium in size, and even 'intimate' in its atmosphere, a range of accommodations are available: guest houses, cottages, rooms in the original inn, and newer rooms in the addition to the inn. All the options are very nice. The cottages, however, offer something special: a private, little house, with sitting room with fireplace, a kitchenette, bedroom, and nicely equipped bathroom.
The meals are excellent: bountiful breakfasts, and multi-course dinners. Plus: you may bring your own wine.
Buckhorn Inn, established in 1938, is a place that one hopes will never change - it adapts gracefully to contemporary standards, but retains the traditional, comfortable, quiet standards of the era in which it was founded.
Serenity in the Smokies
Around Gatlinburg, Tennessee, and other commercial areas of the Smoky Mountains, peace and beauty sometimes seem as illusive as the mists on the mountains; however, both can be found at the Buckhorn Inn. People there are hospitable, but not intrusive. Every breakfast or dinner I have ever eaten there has created a memory. The accomodations are nice, but the air is rarified.
TripAdvisor Reviews Buckhorn Inn Gatlinburg
Travel Blogs from Gatlinburg
Day 6
Blue ridge parkway in the smoky mountains. Rode a stretch that was so narrow and as tight as anything on the dragon. Kent started the day with his shirt on backwards! Ha!! Cool up in the mountains , had to pull out a jacket! Great day! Heading out tomorrow back to Kentucky starting ...
Longer day than expected
... male labs. He was in LOVE with Bruce... but could of cared less about Missy (a smaller mix female)
5) Dolly Parton was everywhere. She has a highway named after her, a theme park... i think a splash park... it's just dolly dolly dolly.
6) Traffic was awful. Awful.
7) Moonshine from TN is no joke... I'm pretty sure you catch a buzz just from walking past the jar....
The night in TN was great, Phil and his family ...
Day 11 - Hiking, wasps, and cinema
... us deeper and deeper into the Appalachian forest, the trail is rather a "deer path", yet all is well and happy - until Robin pulls a stick out of a ground wasp nest, and we have hundreds of wasps all around us! RUN!!!
Robin and Jeanny get sting multiple times, which hurts, and the trail gets so narrow that ...
Day 10 - Quiet day at the farm
Well, it's Sunday after 4th of July. We are a little tired, and just hang out, and finally have some real rest - exactly what we needed!
The kids leave in the morning and aren't seen for the whole day, except checking in when hungry...
There are ...
Day 3 Great Smoky Mountains National Park Loop
... pay everywhere. Luckily Eddie talked the attendant into just paying for 3 spots instead of 5. We walked up to the main drag and chose Howard's based on their sign of being voted best burgers and steaks. It was the only food we've had the entire trip that wasn't great and it was way overpriced. Don't recommend it at all. It was sprinkling when we were done eating so we opted to go to the Hollywood Star Cars Museum. It was a good stop but for the same price I think the Wheels ...


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Nurturing Mind and Body: Nurturing Nest Mineral Hot Springs Retreat and SpaFor those seeking a traditional mind-body cleansing, the five-day Freedom Retreat at Nurturing Nest Mineral Hot Springs Retreat and Spa in Desert Hot Springs may be the perfect destination.
"Our goal is to help our clients reclaim their optimum health," says Ramesh Gune, a psychotherapist who co-directs the program with his wife, Dr. Sandra Gune, a holistic-minded chiropractor. "We do this retreat once a month. It is our signature retreat."
This very personalized getaway is limited to seven people. Couples are asked to book separate times to maximize the benefits — and minimize family spats.
"A lot of emotional garbage comes out during the retreat, so we discourage couples from coming together to avoid any fighting," says Ramesh Gune. "The aim is to clean and detoxify and help people jump-start their lives to be healthier."
You will be in good company. The Freedom Retreat attracts people looking for de-stress solutions from all over the globe, including CEOs and film producers.
So leave your laptop at home, turn off your cell phone, and tune into your body and mind through meditation, yoga, detoxifying and cleansing drinks, one-on-one therapy sessions, scenic hikes, mineral baths, and something called bioenergetic synchronization technique.
The all-inclusive rate for the five-day Freedom Retreat is $2,300.
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subset: commoncrawl


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It's like a crystal ball in the middle of your living room.
Trend Alert: Studs and Rivets
Botega Veneta executive Lisa Pomerantz in the entrance hall of her Manhattan apartment, which was remodeled by architect Peter Pawlak; the C-print is by Thomas Struth, and the stainless-steel bench was costom made.
The living room sofa was inspired by a Jean-Michel Frank design and is upholstered in a Maharam mohair, the vintage Miles van der Rohe Barcelona stools are upholstered in a Donghia wool, and the cocktail table is antique.
Collected Wisdom


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Cringleford Guest House is a delightful property, situated just a short drive from the hospital, University of East Anglia and major roads. The pleasant, well-equipped bedrooms have co-ordinated fabri...More about Cringleford Guest House
Whether you're having fun just messing about on the river or visiting someone at the university, Premier Inn Hotel Cambridge (A14 J32) is a good choice for a visit to the city. From the colleges and t...More about Premier Inn Cambridge (A14 Jct 32)
The Eiders Bed & Breakfast is just a short drive from the centre of Sheringham and is ideally placed for touring the north Norfolk coast. The tastefully appointed bedrooms have lovely co-ordinated fab...More about The Eiders Bed & Breakfast
Situated in the centre of Hunstanton, some rooms benefit from sea views, and all generally provide good levels of comfort. A range of dining options are available with either a bar meal in the Marine ...More about Old Marine Inn
Wiveton Bell is situated in a quiet and scenic part of Norfolk. The luxurious bedrooms come complete with a whole host of accessories including flat-screen TVs, Blu-ray/DVD players, complimentary pros...More about Wiveton Bell
Holm Oaks enjoys a very convenient location on the outskirts of the Georgian village of Holt. The four bedrooms are all attractively presented and free WiFi is available throughout the house. Breakfas...More about Holm Oaks
The Abbey is well placed for visiting the historic town centre. The property is split between several old buildings, the main core dating from the 15th century. The public rooms in the Tudor inn secti...More about The Abbey
Currently the rating for this establishment is not confirmed. This may be due to a change of ownership or because it has only recently joined the AA rating scheme.�� ...More about The King William IV Country Inn & Restaurant
Situated within easy walking distance of the seafront, Caley Hall Hotel offers tastefully decorated bedrooms in a series of converted outbuildings. Each is smartly furnished and thoughtfully equipped....More about Caley Hall Hotel
This lovely village inn provides comfortable, well-equipped bedrooms. A range of quality meals is served in the many dining areas, complemented by a good variety of real ales and wines. Service is fr...More about The Rose & Crown


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Carlisle 4035002 DescriptionSKU: 028-4035002
This 4.5 in. x 7.5 in. nylon scraper made by Carlisle, this Sparta spatula also has a 13.5 in. plastic handle for added convenience. Regardless of what you have need of in a spatula, the Carlisle Sparta spatula is able to accommodate your needs.
Standard Features:
- Sparta spatula
- 4.5 in. x 7.5 in. nylon scraper
- 13.5 in. plastic handle
- White
Sparta Spatula, 13-1/2" long, 7-1/2"L x 4-1/2"W blade, plastic handle, heat-resistant to 300F, dishwasher safe, nylon, smooth finish, white
Carlisle 4035002 Specifications


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These are some designs that I've collected through the years to show the simplicity of my work. My work is very simple and it always works. Heavy animations and the wrong use of colors may hurt your business. I offer clean, aesthetic creations to all of my clients for a more engaging experience.
© 2015 David Duarte Design | Creative Director Call/Text +1-310-595-7011 | Sitemap


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Red Hearts PVC Table Protector
Item Added to Basket
Red Hearts PVC Table Protector is rated out of 5 by 2.
Rated 4 out of 5 by AL from Good value Good looking - protects table tops but still looks attractive. This design nice for Christmas.
Date published: 2014-12-30
Rated 5 out of 5 by Faithful Sally from Tablecloth I bought this product to cover the tables when having functions at several village halls wherever they are needed. It is so much better than the paper banqueting roll we have used which rips. This is the second lot I have bought of the same design, it is so cheerful and bright on the table. Just roll out and cut to length.
Date published: 2014-11-26


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AAPL Stock: 110.78 ( -0.53 )
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Regardless of the recent added pressure from the ThinkPad X300, Apple's MacBook Air is in such high demand that the company is having trouble maintaining the device in stock, according to checks by Ars Technica as well as daily updates from the Mac producer's retail availability page. Both major outlets as well as many smaller, regional shops claim to be selling entire shipments despite receiving new units each day; Apple itself continues to list extended shipping delays for direct orders and ranks the Air as its strongest-selling computer more than a month after early shipments began.
Intel tonight kicked off Germany's CeBIT expo with the unveiling of Atom, a new processor line specifically tailored towards ultra-mobile PCs, mobile Internet devices (MIDs), and other handhelds. Previously nicknamed either Silverthorne or Diamondville, the processor series is built on the same 45 nanometer manufacturing process as newer Core 2 chips and shares the same instruction set, but is far smaller: a single US penny is large enough to fit 11 Atom processor dies, Intel touts. While simpler at 47 million transistors, this and size reduction techniques reduce its power use to between 0.6 and 2.5 watts, enough to fit in very small spaces.
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Wedding Cake Makers Cheltenham
(5) for Wedding Cake Makers in Cheltenham
The Cheltenham Cake Co3, Dunster GardensGL51 0QT Cheltenham01242 251501
... decoration, decorators, Cake Makers Decorators, cake, cake decorator, wedding cake maker, bakery
Pryers Kakes & Supplies14 Bennington StreetGL50 4ED Cheltenham01242 524129Closed
... cake decorator, wedding cake maker, cake icing, cake baker, Cake Makers, cake decorating equipment...
Request for Quotation
Are you looking for Cake Makers & Decorators ?Use our "Request for Quotation" service to get customized offers from companies in Cheltenham, tailored for your exact needs!
Cheltenham Cake Co1 Prestbury RoadGL52 2PN Cheltenham Prestbury01242 253978
... baker, bakery, wedding cake maker, cake icing, cake decorator, Cake Makers Decorators, birthday...
Pinny For Your Thoughts8 New Barn AvenueGL52 3LJ Cheltenham01242 515262
... cakes, Wedding
Harlequin Cakes of Cheltenham24 New Barn CloseGL52 3LW Cheltenham Prestbury01242 257613Closed
Specialising in beautiful bespoke Wedding Cakes, also birthday cakes, christening and anniversary cakes. Stunning designs and flavours. There is always an occasion to celebrate with cake!
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Five Valleys Celebration Cakes39, Allen DriveGL5 4DT Stroud07810 890157
Five Valleys Cakes make bespoke cakes for any occasion. All our cakes are individually designed and made to your requirements. Special requirements such as organic, vegan or allergy free cakes can be...
L.f.dangerfield (Bakery) LtdDangerfields Bakery, Church StreetGL10 3HT Kings Stanley01453 822540
Dangerfields Bakery a family run bakery producing high quality bread, morning goods, confectionery and celebration cakes. ... BAKERY, bread, pies, morning goods, mince, wedding cakes, mince pies, loaf, confectionery supplies...
Cocoa Daisy CupcakesMilbury Mews, Bath RoadGL10 3LU Leonard Stanley07581 564003Closed
... vanilla, daisy, bespoke, cupcakes
The Sugar Craft Shop4 Barnard ParadeGL10 2JA Stonehouse01453 828885
cake design and Sugarcraft equipment. With an open mind for learning and interest for all things new that come onto the market I expanded my knowledge in the Sugarcraft industry too. I enjoy what I...
Joanna Parker Catering and EventsThyme CottageGL6 9HB Box01453832715
Joanna Parker Catering and Events is Gloucestershire based private catering and events company. We have clients around Stroud, Cirencester, Gloucester, Cheltenham, Bristol, Bath, Swindon and central ... Cheltenham caterers, canape parties, wedding caterer, executive lunches, caterers Gloucester,...
Sweet HeartsBath Road 2GL6 0BB Nailsworth01453 835266
... wedding cupcakes, Wedding Favours
Janette S Celebration CakesBluebell Cottage, Bluebell RiseGL6 8NP Chalford01453 886766
cake can alter the price. Once you have an idea in mind, make an appointment and bring along any pictures you have and swatches of the gown, colour schemes and flowers you are thinking of having. A...
Mmm CoffeeJasmine Cottage, Green LaneGL2 7LY Moreton Valence01452 729398
MMM! Coffee offers catering for outdoor events throughout Gloucestershire. Our coffee van holds hot drinks and delicious snacks for all tastes!
Pat-a-Cakes Catering ServicesUnit H8, Draycott Business Park, CamGL11 5DQ Cam01453 702649
... Catering Services are caterers in Cam, Gloucestershire, we specialise in being event caterers, wedding caterers, party caterers. If you are holding an event in Gloucestershire and nearby we are the...
Novelty Cakes1 Mildenhall Way, KingswayGL2 2DH Quedgeley01452 722659Closed
cake making and decorating career after finishing a comprehensive cake making course back in 2000. After a three year break, which she devoted for her graduation studies she is back in business. She...
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Haversham Village School
- Name:
- Haversham Village School
- Address:
- The Crescent
- Postcode/City:
- MK19 7AN Haversham
- County:
- Buckinghamshire England
- Phone:
- 01908 31267301908 312673
- Website:
- www.havershamvillageschool.co.uk
- E-mail:
- Send message
Updated 3 months ago | Claim This Business| Report Incorrect Data
Haversham Village School is an outstanding school. Pupils of all abilities achieve well. Parents and carers and pupils appreciate the high-quality care and exciting learning opportunities. A rural school with spacious, attractive grounds, which is part of a vibrant, village culture. A strong, dedicated and caring team of staff committed to helping your child reach their full potential.
Keywords:School, Teaching, Local Authority Schools, Educational Institution, Preparatory School, Children Education, Educational Establishments, Independent Schools, Lessons, Learning, Classes, Teachers
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Shop Owner
Have a question?Contact Shop Owner
Shop Info
Opened on Sep 19, 2010
- About
- Policies
- Seller Information
- Reviews
- 248 sales
- 605 admirers
Custom passport covers, travel gifts also for kids
BagsByTravelher's Shop Policies
Thanks for stopping by! Welcome in my shop.
Everything you see in my shop is designed and made by me. For this I use the very best quality of fabrics I find all over the world. I do not mass produce my bags. Of every item I just make a few, you can be sure to possess a very unique item! What I like to do the most is combine fabrics of different origins. They often make great matches en thus tell a great story!
I accept payments through Paypal, Credit cards and Ideal. After you payment has checked out your ordered items will be shipped to you. I would like to have your Payment within 24 hours after placing your order.
If you request a custom made item I will list a extra Etsy item in my shop, You can thus make your payment through the normal channel. The prices you see in my Etsy shop are the prices before Dutch taxes. If you order from outside the European union, no taxes will apply to your order. If you order from an EU country 19% taxes apply. This will be automatically added.
If your order has been a major mistake you can cancel within 24 hours. I will then refund your complete payment.Please check with me first, id I already shipped hour item cancellation is not possible.
Customized and personalized orders cannot be cancelled.
I ship within 2 or 3 days with a normal carrier. I you wish me to send you a secured package, this is possible at additional costs. I can send you a quote for the extra shipping costs.
From the Netherlands shipping is quite smooth. Most packages arrive in the USA within a week, Australia takes approximately 2 weeks. packages sent to European countries take less then a week.
Refunds and Exchanges
I you received a damaged item, or do not receive an item at all, do contact me immediately. This rarely happens, but when it happens I intend to make the problem go away and leave the customer with smile.
Customized bags or personalized items cannot be resold by me, and cannot be returned.
Additional Policies and FAQs
You can contact me for custom order or personalized items.
For certain items I can provide you with wholesale prices (e.g. passport cover for destination wedding presents)
Seller Information
Monique Rhuggenaath
the Netherlands
info [!at] travelher.nl
Last Updated June 27, 2014


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Heidi Klein's sky-blue bandeau bikini has a flattering twisted top. The molded cups and boned sides offer subtle support. Wear this strapless style with a loose shirt when seeking the shade.
Sky-blue slub stretch polyamide and polyester-blendTwisted top, molded cups, boned sidesRuched waistband Fully linedTies at back of top45% polyamide, 37% polyester, 18% elastaneHand wash or dry clean
Product code: 407808 - Need help? Contact us


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FLEX Dual Action & Rotary Machine Polishers are designed and built in Germany to exacting standards. A favourite among detailing professionals and enthusiasts alike they are the preferred choice of Porsche Body Shops and RUF Automobiles GmbH.
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Lois came to Washington to always be on your side, and stand up for all of South Florida's middle class families. But the GOP has already indicated that they are going to throw everything they can at her in a effort to gain control of her seat.
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The title is a little confusing so let me explain better what the problem I'm having is.
I need to extract a certain portion of HTML out of a page. This portion of code is inside of a div that "on page load" is hidden by default. You have to click on that div in order to make that portion of code appear.
Now, I need to get this code with a javascript/jquery script with either pure AJAX request to the page or YQL but the problem is: How do I "simulate" the click on that div?
How can I make that div toggle just with the code in order to access the code inside of it?
By the way, the request is from the same domain so there's no problem with cross-domain AJAX.
Thank you!


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Alterations in gene expression in peripheral blood cells have been shown to be sensitive to the presence and extent of coronary artery disease (CAD). A non-invasive blood test that could reliably assess obstructive CAD likelihood would have diagnostic utility.
Microarray analysis of RNA samples from a 195 patient Duke CATHGEN registry case:control cohort yielded 2,438 genes with significant CAD association (p < 0.05), and identified the clinical/demographic factors with the largest effects on gene expression as age, sex, and diabetic status. RT-PCR analysis of 88 CAD classifier genes confirmed that diabetic status was the largest clinical factor affecting CAD associated gene expression changes. A second microarray cohort analysis limited to non-diabetics from the multi-center PREDICT study (198 patients; 99 case: control pairs matched for age and sex) evaluated gene expression, clinical, and cell population predictors of CAD and yielded 5,935 CAD genes (p < 0.05) with an intersection of 655 genes with the CATHGEN results. Biological pathway (gene ontology and literature) and statistical analyses (hierarchical clustering and logistic regression) were used in combination to select 113 genes for RT-PCR analysis including CAD classifiers, cell-type specific markers, and normalization genes.
RT-PCR analysis of these 113 genes in a PREDICT cohort of 640 non-diabetic subject samples was used for algorithm development. Gene expression correlations identified clusters of CAD classifier genes which were reduced to meta-genes using LASSO. The final classifier for assessment of obstructive CAD was derived by Ridge Regression and contained sex-specific age functions and 6 meta-gene terms, comprising 23 genes. This algorithm showed a cross-validated estimated AUC = 0.77 (95% CI 0.73-0.81) in ROC analysis.
We have developed a whole blood classifier based on gene expression, age and sex for the assessment of obstructive CAD in non-diabetic patients from a combination of microarray and RT-PCR data derived from studies of patients clinically indicated for invasive angiography.
Clinical trial registration information
- i
Keywords:Atherosclerosis; gene expression; whole blood classifier
The promise of genomics to improve diagnosis and prognosis of significant diseases is dependent on a number of factors including appropriate use of technology, identification of clinical issues of significant unmet need, and the rigorous statistical derivation and testing of genomic classifiers[1]. Multigene expression classifiers have been developed and have become incorporated into clinical guidelines in both breast cancer recurrence prognosis and heart transplant rejection monitoring[2,3]. A guideline for the metrics such classifiers should meet, including independent validation, and adding to current clinical factor algorithms has been described [4] and it has been suggested that peripheral blood cell gene expression may reflect pathological conditions in a variety of cardiovascular disease states[5]. In this work we describe the development of a validated whole blood based classifier for the assessment of obstructive CAD[6].
Mortality and morbidity from CAD and myocardial infarction (MI) are a major global health burden. Major determinants of current CAD likelihood are sex, age, and chest-pain type [7,8]. Other risk factors such as diabetes, smoking, dyslipidemia, hypertension and family history have been associated with future cardiovascular event risk[9]. In addition, atherosclerosis has a systemic inflammatory component including activation and migration of immune cells into the vessel wall[10,11]. Prior work has shown that quantitative measurements of circulating blood cell gene expression reflect the extent of CAD[12,13]. These observations likely reflect both changes in cell type distributions, which have prognostic value for cardiovascular events [14] and gene expression changes within a specific cell type or lineage.
The "gold standard" for detecting CAD is invasive coronary angiography; however, this is costly, and can pose risk to the patient. Prior to angiography, non-invasive diagnostic modalities such as myocardial perfusion imaging (MPI) and CT-angiography may be used, however these only add moderately to obstructive CAD identification[15]. We describe herein the development of an algorithm for the assessment of obstructive CAD using peripheral blood gene expression, age, and sex, which was subsequently validated in an independent cohort[6].
Patient selection and clinical methods
All patients were clinically referred for angiography and angiograms were performed based on local, institutional protocols. The first microarray cohort of 198 subjects (88 cases and 110 controls) was derived from the Duke University CATHGEN registry, a retrospective blood repository, enrolled between August 2004 and November, 2005 [16]. For CATHGEN patients, clinical angiographic interpretation defined cases as ≥75% maximum stenosis in one major vessel or ≥50% in two vessels and controls as <25% stenosis in all major vessels. Clinical inclusion and exclusion criteria were described previously and included both diabetic and non-diabetic patients [13]. All CATHGEN patients gave written informed consent and the study protocol was approved by the Duke University IRB.
The second microarray cohort of 210 subjects (105 case: control pairs, matched for age and sex) and the RT-PCR algorithm development cohort (210 cases and 430 controls) were part of PREDICT, a multi-center US study of patients referred for coronary angiography (http://www.clinicaltrials.gov webcite, NCT00500617). For PREDICT patients, core laboratory QCA reads (Cardiovascular Research Foundation New York) were used for case: control classification. Cases had ≥50% stenosis in at least one major coronary vessel and controls <50% stenosis in all major vessels.
Subjects from PREDICT were eligible if they had a history of chest pain, suspected anginal-equivalent symptoms, or a high risk of CAD with no known prior MI, revascularization, or CAD. Detailed inclusion/exclusion criteria have been described [6]. Diabetic status was defined by clinical identification, blood glucose (non-fasting ≥200 or fasting ≥126), rorhemoglobin A1c, (≥6.5), or diabetic medication prescription. Complete blood counts with differentials were obtained for all patients. PREDICT patients gave written informed consent, and the study protocol was approved by the Institutional Review Boards.
Blood collection, RNA purification and RT-PCR
Whole blood samples were collected in PAXgene® tubes prior to coronary angiography, according to the manufacturer's instructions, then frozen at -20°C. For the CATHGEN samples RNA was purified as described (PreAnalytix, Franklin Lakes, NJ), followed by quantitative analysis (Ribogreen, Molecular Probes, Eugene, OR). For the PREDICT samples an automated method using the Agencourt RNAdvance system was employed.
Correlation between gene expression and cell type distributions
Correlations with complete blood counts and database gene expression analysis (SymAtlas, http://biogps.gnf.org webcite) were used to identify highly cell-type selective genes. In addition, whole blood cell fractionation by density centrifugation or through positive antibody selection followed by RT-PCR was performed on specific cell fractions (see Additional file 1).
Additional file 1. Algorithm Score Calculation and Transformation. Cell fractionation and cell specific gene expression analysis.
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Statistical methods
All statistical methods were performed using the R software package.
Microarray methods
Microarray samples were labeled and hybridized to 41K Human Whole Genome Arrays (Agilent, PN #G4112A) using the manufacturer's protocol. For PREDICT microarrays all matched pairs were labeled and hybridized together to minimize microarray batch effects. Microarray data sets have been deposited in GEO (GSE 20686).
Agilent processed signal values for array normalization were scaled to a trimmed mean of 100 and then log2 transformed. Standard array QC metrics (percent present, pair-wise correlation, and signal intensity) were used for quality assessment, resulting in 3 of 198 CATHGEN and 12 of 210 PREDICT samples being excluded.
Array analysis
For the CATHGEN array, logistic regression (unadjusted and sex/age adjusted) was used to assess gene expression association with case: control status. For the PREDICT array, given the paired design, conditional logistic regression was used. False discovery rates were used to account for multiple comparisons. BINGO was used to assess enrichment of gene ontology terms in the set of 655 genes[17]. A hyper-geometric test was used to identify overrepresented terms; results were corrected for multiple testing using Benjamini & Hochberg False Discovery Rate (FDR) correction.
Gene selection
Genes for RT-PCR were selected based on statistical significance, gene ontology pathway analysis, and literature support. Hierarchical clustering based on gene: gene correlations ensured that RT-PCR genes represented multiple clusters. Normalization genes were selected based on low variance, moderate to high expression, and no significant association with case: control status, sex, age, or cell counts. Cell-type genes were selected based on known literature or correlation to known cell-type specific markers.
PCR methods
Amplicon design, cDNA synthesis, and RT-PCR were performed as previously described [6,13]. All PCR reactions were run in triplicate and median values used for analysis. Clinical/demographic factors were assessed for CAD association using univariate and multivariate logistic regression. Gene expression association with CAD and other clinical/demographic factors was assessed by robust logistic regression (unadjusted and sex/age adjusted) [13].
Algorithm development and validation
Hierarchical clustering was used to group genes using a correlation cutoff. Clusters were reduced to meta-genes[18] and normalization genes based on correlation structure, known biology, and cell count correlation. In general, a meta-gene was a set of 1-4 genes from a specific cluster, chosen to best represent the cluster center using a parsimonious number of genes. Genes within meta-genes were equally weighted with one exception (Additional File 1). For meta-gene pairs with high correlation and opposite disease regulation, ratio terms (differences on the log scale) were defined. Meta-genes independently associated with outcome were selected by the LASSO method, with sex by meta-gene interactions allowed during variable selection [19].
The final algorithm was fit using Ridge regression [20], where the outcome variable was case: control status and the predictors the LASSO-selected meta-genes and sex-specific age terms. Sex was a binary predictor, and age a linear predictor with separate slopes for men, women >60, and women <60 (the slope for women age < 60 was estimated to be approximately 0 and thus was set to 0 in the final algorithm). The LASSO was fit using the glmnet package in R and ridge regression was fit using the Design package in R; in both cases the shrinkage parameter lambda was estimated using 10-fold cross validation. Model performance was estimated using leave-one-out cross-validation.
A schematic of the patient, gene, and logic flow for gene discovery and algorithm development is shown in Figure 1. Baseline demographic characteristics of the CATHGEN registry and PREDICT study microarray patient cohorts are summarized in Table 1. Significant clinical and demographic factors for obstructive CAD were age, male sex, systolic blood pressure, and dyslipidemia; increased neutrophil count and decreased lymphocyte count trended toward significance. In all cases whole blood samples were obtained in PAXgene® tubes and microarray analysis performed using the Agilent 41K platform.
Figure 1. Gene discovery and algorithm development patient and logic flow schematic. Initial gene discovery (CATHGEN repository) included both diabetic and non-diabetic patients. Gene discovery from PREDICT involved non-diabetic patients in a paired microarray analysis, that yielded 655 significant genes in common with those from the CATHGEN arrays. For RT-PCR 113 genes were selected and tested on 640 PREDICT patient samples, from which the final algorithm was derived and locked.
Table 1. CATHGEN and PREDICT microarray cohort clinical and demographic characteristics
A total of 2,438 genes showed significant CAD association (p < 0.05) in the 195 subject case: control analysis from the CATHGEN cohort (Figure 1). Analysis of the effect of clinical factors on gene expression showed diabetes as the most significant (p = 0.0006, Additional file 2). Based on statistical significance and biological relevance, 88 genes (Additional file 2) were selected for RT-PCR analysis on these same samples. CAD-gene expression analysis in non-diabetic and diabetic subsets (N = 124 and 71, respectively), showed 42 and 12 significant genes, respectively (p < 0.05), with no intersection (Figure 2). Further work was thus limited to non-diabetics.
Additional file 2. Data Tables. Table S1 - Significance of Clinical Variables in CATHGEN gene discovery cohort. Table S2 - Significance of RT-PCR results for the 88 genes tested in the CATHGEN discovery cohort, in the non-diabetic and diabetic subsets. Table S3 - The 655 genes identified in both the CATHGEN and PREDICT discovery microarray experiments. Table S4 - The significant biological process, cellular compartment and molecular function ontologies from GO analysis of the 655 genes.
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Figure 2. RT-PCR analysis of diabetic status impact on significant genes from CATHGEN microarray analysis. Significance of individual genes selected from the CATHGEN microarray cohort in non-diabetic (ND) and diabetic (D) patients is shown. The sex/age adjusted p values from a CAD logistic regression analysis in each subset are plotted (log scale). Significant p values (<0.05) are indicated in red with gene symbols, non-significant ones in black.
Microarray CAD gene discovery on 210 PREDICT non-diabetic patient samples used a paired case: control experimental design, to reduce confounding effects of age, sex and microarray batch processing. CAD analysis on the 99 case: control pairs which passed quality metrics yielded 5,935 significant genes (p < 0.05) with 655 genes in common with the CATHGEN results (Figure 3, Additional File 2). Gene Ontology (GO) analysis of these 655 genes identified 55 significant, overrepresented biological process terms (adjusted p < 0.05, Figure 4, Additional File 2), largely reflecting inflammation, immune cell differentiation, cell death and apoptosis. The molecular and cellular ontologies showed enrichment of 3 and 10 terms respectively, including caspase activity and ribosomal function.
Figure 3. Venn diagram of microarray, RT-PCR, and algorithm gene sources. A total of 7718 genes were identified, 2438 and 5935, respectively, from the CATHGEN and PREDICT microarray analyses, with an intersection of 655 genes. For the 113 RT-PCR genes, 52 were from PREDICT, 22 from CATHGEN, and 29 from both; 10 were either normalization genes or from previous studies [13]. The final algorithm contained 20 informative genes: 10 from both microarray studies, 8 PREDICT alone, and 2 CATHGEN alone.
Figure 4. Gene ontology analysis of 655 CAD genes identified from microarray studies. The 655 CAD genes identified were analyzed using the BINGO algorithm to ascertain significant biological processes. Significant processes (p < 0.01 after FDR correction) are colored with the gradient of p values reflected in the colors as indicated, and the biological process annotated. A total of 55 processes were significant in this analysis at p < 0.05.
Gene selection
A total of 113 genes (Table 2) were selected by statistical significance, biological relevance, and prior association with CAD from RT-PCR gene expression measurements in the 640 patient PREDICT algorithm development cohort (Figure 1, Table 3). Known cell-type specific markers, those correlated with cell counts in PREDICT, and candidate normalization genes, were also represented.
Table 2. Genes evaluated by RT-PCR in the algorithm development cohort
Table 3. PREDICT algorithm development cohort clinical and demographic characteristics1
Analysis of algorithm development cohort: clinical and gene expression factors
The most significant clinical/demographic factors for CAD association were age, sex, chest pain type and neutrophil count. Age and sex were independent risk factors for CAD (Table 3) and showed significant gene expression correlation. Chest pain type was also a significant independent risk factor (p = 0.0004), especially in men, but was gene expression independent. Neutrophil count was significantly correlated (positively or negatively) to expression of 93 of 113 RT-PCR genes, and was significantly associated with CAD in men (p = 0.049) but not women (p = 0.77). Neutrophil-associated genes showed both up and down regulation with CAD status, whereas lymphocyte-associated genes were generally down-regulated. There was significant gender-specific regulation of neutrophil correlated genes (men 40/42 genes up-regulated, women, 41/42 down-regulated) whereas lymphocyte gene down-regulation was gender independent.
Hierarchical clustering of the 113 PCR genes resulted in 18 correlated clusters (Figure 5, Table 2), a significant fraction of which could be mapped to cell-type specific gene expression groups, with finer correlation substructure within the lymphocyte and neutrophil associated genes. There were 3 lymphocyte subgroups representing T-cells (clusters 1,2,3), B-cells (cluster 3) and NK cells (cluster 12). Three neutrophil subgroups were also identified: previously described neutrophil genes (IL8RB, S100A8, S100A12, TXN, BCL2A1; cluster 13, 16); newly identified up-regulated neutrophil genes (CLEC4E, CASP5, TNFAIP6; cluster 16) and down-regulated neutrophil genes (KCNE3, TLR4, TNFRSF10C; clusters 13, 14) [13]. Cluster 8 appears to be eosinophil specific. The 26 genes in clusters 4-7 and 9-11 did not have clear cell-type association.
Figure 5. Heat-Map representation of Hierarchical Clustering Results on 113 RT-PCR Genes. Clusters were generated by hierarchical clustering yielding 20 groups of correlated. Clusters were annotated as to cell type expression using BioGPS (http://www.biogps.gnf.org webcite). Extent of correlation is indicated by color as shown in the bar.
Algorithm derivation and performance
Based on gene expression correlation clustering and cell-type analyses, 15 meta-genes and 3 normalization genes were defined as inputs for model variable selection (Table 2, Figure 6). Selection by the LASSO method and further weight penalization by Ridge regression resulted in the final, locked algorithm, comprising 20 CAD-associated genes and 3 normalization genes in 6 meta-gene terms (Figure 6), where each term represents a ratio of meta-genes or meta-gene to normalization genes. The algorithm score is calculated as described (Additional file 1) and was defined as the predicted regression model value.
Figure 6. Schematic of the final algorithm structure and genes. The algorithm consists of overlapping gene expression functions for men and women with a sex-specific linear age function for the former and a non-linear age function for the latter. The genes in each term and their weights are shown. For the gene expression components, 16/23 genes in 4 terms are gender independent: Term 1 - neutrophil activation and apoptosis, Term 3 - NK cell activation to T cell ratio, Term 4, B to T cell ratio, and Term 5 -AF289562 expression normalized to TFCP2 and HNRPF. In addition, Term 2 consists of 3 sex-independent neutrophil/innate immunity genes (S100A8, S100A12, CLEC4E) normalized to overall neutrophil gene expression (AQP9, NCF4) for women and to RPL28 (lymphocytes) for men. The final male specific term is the normalized expression of TSPAN16. Algorithm score is calculated as described (Additional file 1).
The estimated cross-validated algorithm AUC in ROC analysis in the PREDICT development set was 0.77 (95% CI 0.73 to 0.81) (Figure 7); prospective validation in an independent PREDICT validation set of 526 patients (192 cases, 334 controls) yielded an AUC of 0.70 (95% CI = 0.65 to 0.75) [6].
Figure 7. ROC analysis of final algorithm. The cross-validated ROC curve for the final algorithm in the algorithm development cohort is shown. The AUC is 0.77 ± 0.04.
This study presents gene discovery from microarrays and development from a large RT-PCR data set of a whole blood derived RT-PCR based gene-expression algorithm for assessment of obstructive CAD likelihood in non-diabetic patients, which was subsequently validated in an independent patient set [6].
The limitation to non-diabetic patients was due to the significant differences observed in PCR-based technical replication of the initial microarray experiment from the CATHGEN cohort, where both diabetic and non-diabetic patients were included (Figure 2). This effect could be due to differences in the pathophysiology of CAD in diabetics, as has been observed at the plaque composition level, [21] or due to diabetic medications, some of which modulate gene expression and affect cardiovascular disease [22].
A number of methodological steps deserve highlighting: first, we interrogated whole blood samples from more than 1,000 patients; second, we developed and used an automated and high reproducible RNA extraction process for the PREDICT samples; third, for the PREDICT work we also used core laboratory determined quantitative coronary angiography to define maximum percent stenosis and case: control status and fourth, we used ratios of correlated gene sets or meta-genes as building blocks for algorithm development. These methodological approaches enhanced the power of the PCR algorithm development set to investigate the relationship between CAD, clinical factors, and gene expression.
The relationships between age, sex, CAD, and gene expression are complex. Increasing age and male sex are well-known risk factors for CAD, which affect gene expression in circulating cells [23,24]. The majority of genes measured by RT-PCR in this study correlated with lymphocyte or neutrophil fraction. Lymphocyte-associated gene expression decreases with CAD in a sex-independent fashion, consistent with decreased lymphocyte counts being correlated with increased cardiovascular risk [14]. In contrast, neutrophil-associated genes display significant sex-specific expression differences with CAD: in men 95% of the neutrophil genes were up-regulated whereas 98% were down-regulated in women, consistent with increased granulocyte counts in men being associated with higher CAD risk, with lesser effects in women [25,26].
Biological significance of algorithm terms
The use of correlated meta-genes as building blocks for the algorithm is significantly reflective of gene expression cell-type specificity. The algorithm genes are expressed selectively in multiple types of circulating cells including neutrophils, NK cells, B and T-lymphocytes, [27], supporting roles for both adaptive and innate immune responses in atherosclerosis [10].
A role for neutrophils in both the early and later stages of atherogenesis has recently been suggested, especially in connection with hyperlipidemia [28,29]. Algorithm term 1 is a ratio of neutrophil expressed meta-genes that are up and down regulated with CAD (Figure 6). This term may particularly reflect neutrophil apoptosis, as Caspase-5 is increased with CAD, whereas TNFRSF10C, an anti-apoptotic decoy receptor of TRAIL, is decreased [30]. Term 2 genes up-regulated with CAD are also expressed largely by neutrophils and likely reflect both innate immune activation, (S100A8 and S100A12), [31] and a cellular necrosis response (CLEC4E) [32]. S100A8 and S100A12 are up-regulated in chronic inflammatory conditions, including asthma, rheumatoid, and inflammatory arthritis, perhaps reflecting a more general pathophysiological signal, consistent with increased CAD in disorders such as rheumatoid arthritis [33,34].
Term 2 is highly correlated with the signature previously identified by us [13] and includes the most significant gene from that work, S100A12. This term is normalized in a sex-specific manner, perhaps reflecting sex-specific differences in the significance of neutrophil counts in CAD and MI [26]. In men normalization to RPL28 which is strongly expressed in lymphocytes, reflects the neutrophil to lymphocyte ratio, which is prognostic for death or MI in a CAD population [14]. In women normalization to AQP9 and NCF4, two CAD insensitive neutrophil genes, permits assessment of neutrophil up-regulation of the S100s and CLEC4E, independent of neutrophil count.
Term 3 consists of 2 NK cell receptors, SLAMF7 and KLRC4, normalized to T-cell specific genes (TMC8 and CD3D). SLAMF7 may specifically activate NK cell function, while inhibiting B and T cells [35]. KLRC4 is also likely involved in NK cell activation [36]. NK cells have been associated with atherosclerosis in both mouse models and humans, and reduced lymphocyte counts associated with cardiac events [14,37].
Term 4 is a gene expression based measure of the B/T-cell ratio. The roles of both T and B cells in atherosclerosis development are complex; mouse models have shown B cells to be both athero-protective and more recently, atherogenic [38-40]. In this study apparent upregulation of B-cell specific genes is correlated with CAD, perhaps supporting the latter. The last two terms, based on AF289562 (AF2) and TSPAN16 are genes of unknown function that will require further work to clarify their role in atherosclerosis.
Previous work by Sinnaeve and coworkers also examined peripheral blood gene expression in a coronary disease population [12]. As noted by these authors, there is little overlap between their genes and the signatures identified in our previous study [13] or this one. A number of significant differences in the study populations (restricted age range, combining two sex specific cohorts) in their study may have contributed to this. In addition, there are differences in both RNA isolation methodology and microarray platforms. Further work is needed to resolve these issues.
Algorithm development
For algorithm development, as described above, we used an approach that minimized the effect of any single gene by using meta-genes as building blocks [18,41] Penalized stepwise logistic regression (LASSO) selected significant meta-genes from a 640 patient data set which greatly exceeded the number of candidate variables (15 meta-genes), reducing the likelihood of over-fitting. Further, in order to minimize over-weighting of individual terms, meta-gene coefficients were penalized using Ridge regression. An alternative approach would have been to use a combined two-step shrinkage method such as the elastic net [42]. Although correlations with specific cell types was a key observation, recent methodologies described for deconvoluting gene expression data sets from complex cell mixtures might have led to improved results [43].
The cross-validated model AUC was 0.77 (95% CI 0.73 to 0.81), suggesting that the algorithm score was a significant CAD predictor. A decrease to an AUC of 0.70, with overlapping confidence intervals (95% CI = 0.65 to 0.75), was observed in the independent validation set [6]. This decrease may reflect an over-optimistic cross-validation estimate, as we did not re-select terms during each iteration. Ultimately, the validation results provide the most informative measure of a model's prediction accuracy.
Although this is one of the largest studies examining gene expression in peripheral blood in CAD patients and has yielded a specific algorithm for the assessment of CAD status, it has several limitations.
From a clinical perspective, diabetics and patients with known chronic inflammatory disorders were excluded. The differences observed between diabetics and non-diabetics with CAD could be due to differences in the molecular pathophysiology of the disease, medications, or some combination of the two. In addition, although race was not an independent risk factor after adjustment for age and sex, the number of minority patients was low, so conclusions with respect to them are significantly underpowered. The use of a dichotomous angiographic endpoint does not account for variations in disease burden or external remodeling, and is not a measure of ischemia. Finally, the contribution of atherosclerosis in other vascular beds is outside the scope of this study, but may be important in asymptomatic high-risk individuals.
From a cellular and gene expression perspective, the relative ease of obtaining peripheral blood cell RNA is counter-balanced by not directly interrogating changes in the diseased vascular wall. Another complementary approach could be to examine secreted proteins in the blood that might reflect endothelial or vascular dysfunction. Finally, given the chronic nature of atherosclerotic disease, it is likely the gene expression signature observed reflects a response to disease rather than the underlying cause.
Using a series of microarray and RT-PCR data sets, comprising more than 1,000 patients, we have derived an algorithm, consisting of the expression levels of 23 genes, sex, and age, which can assess the likelihood of obstructive CAD in non-diabetic patients.
CAD: coronary artery disease; MI: myocardial infarction; MPI: myocardial perfusion imaging; RT-PCR: real-time polymerase chain reaction; QCA: quantitative coronary angiography; ROC: receiver-operator characteristics; AUC: area under the curve.
Competing interests
This work was funded by CardioDx, Inc. MRE, JAW, PB, SED, and SR are employees of CardioDx, Inc and have equity interests and/or stock options in CardioDx. WGT is a former employee and has equity or stock options in CardioDx. SR, MRE, JAW, PB and WGT have filed patent applications on behalf of CardioDx, Inc. WEK reports research support from CardioDx. EJT is supported in part by the Scripps Translational Science Institute Clinical Translational Science Award (NIHU54RR02504-01). AJL reports funding from CardioDx to complete the QCA studies reported herein. RSS, SZ, RW, JM, and NT report no conflicts of interest with respect to the contents of this manuscript.
Authors' contributions and information
MRE, PB, JAW, SED, WGT, SR, SZ, GG, AL, WEK, RSS, and EJT contributed to the Conception, Design, and Data Analysis for this work as well as drafting and approving the final manuscript. SE, NT, RT, and JM helped critically revise the manuscript and all authors approved the final version. The remaining PREDICT investigators are listed in the Acknowledgements.
Acknowledgements and funding sources
This work was funded by CardioDx, Inc. The funding source was involved in the design, execution and analysis of the study in concert with the PREDICT Executive Committee (EJT, WEK, RSS, and SV): The authors gratefully acknowledge the contributions from the PREDICT Site Principal Investigators: Daniel Donovan, Cardiology Clinic of San Antonio, San Antonio, TX; Stanley Watkins, Alaska Heart Institute, Anchorage, AK; Brian Beanblossom, Cardiovascular Associates, Louisville, KY; Brent Muhlestein, Intermountain Health Care, Salt Lake City, UT; Ronald Blonder, Pikes Peak Cardiology, Colorado Springs, CO; Tim Fischell, Borgess Research Medical Center, Kalamazoo, MI; Phillip Horwitz, University of Iowa Hospitals, Iowa City, IA; Frank McGrew, The Stern Cardiovascular Center, Germantown, TN; Tony Farah, Allegheny Professional Building, Pittsburgh, PA; Terrance Connelly, Charlotte Heart Group Research Center, Port Charlotte, FL; Cezar Staniloae, New York Cardiovascular Assoc./Heart and Vascular Research, New York, NY; Edward Kosinski, Connecticut Clinical research, LLC, Bridgeport, CT; Charles Lambert, University Community Health, Tampa, FL; David Hinchman, St Luke's Idaho Cardiology Associates, Boise, ID; James Zebrack Heart Center, Salt Lake City, UT; Bruce Samuels, Cardiovascular Medical Group of Southern CA, Beverly Hills, CA; Matthew Budoff, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA; Dean Kereiakes, The Lindner Clinical trial Center, Cincinnati, OH; Christopher Brown, Mobile Heart Specialists, Mobile, AL; Jennifer Hillstrom, Maine Cardiology, Portland, ME; Donald Wood, Peninsula Cardiology Associates, Salisbury, MD; Hossein Amirani, Via Christi Research, Wichita, KS; Jeffrey Bruss, Hoag Heart & Vascular Institute, Newport Beach, CA; Ronald Domescek, Orlando Heart Center, Orlando, FL; Stephen Burstein, Los Angeles Cardiology Associates, Los Angeles, CA; Mark Heckel, Carolina Heart Specialists, Gastonia, NC; Barry Clemson, Heartcare Midwest SC, Peoria, IL; Charles Treasure, Cardiovascular Research Foundation, Knoxville, TN; Ricky Schneider, Cardiology Consultants of South Florida, Tamarac, FL; Hassan Ibrahim, North Ohio Heart Center, Sandusky, OH: Robert Weiss, Maine Research Associates, Auburn, ME; John Eagan, Jr, Office of Clinical Research, Birmingham, AL; David Henderson, Cardiology Research Associates, Ormond Beach, FL: Lev Khitin, Chicago Heart Institute, Elk Grove, IL; Preet Randhawa, New Jersey Heart Research, Linden, NJ.
In addition, we acknowledge all the patients who provided samples for the PREDICT study as well as the study site research coordinators and those who contributed to patient recruitment, clinical data acquisition and verification, validation study experimental work and data analysis, including the contributions from the CardioDx teams: Bradley Brown, Lori Littleford, Amy Sehnert, Clinical Operations and Research; Karen Fitch, Heng Tao, Rachel Nuttall, Michael Doctolero, Research and Development; Jon Marlowe, Laboratory Automation.
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Proc Natl Acad Sci USA 2004, 101(16):6062-6067. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Drechsler M, Megens RT, van Zandvoort M, Weber C, Soehnlein O: Hyperlipidemia-Triggered Neutrophilia Promotes Early Atherosclerosis.
Zernecke A, Bot I, Djalali-Talab Y, Shagdarsuren E, Bidzhekov K, Meiler S, Krohn R, Schober A, Sperandio M, Soehnlein O, et al.: Protective role of CXC receptor 4/CXC ligand 12 unveils the importance of neutrophils in atherosclerosis.
Circ Res 2008, 102(2):209-217. PubMed Abstract | Publisher Full Text
Hasegawa H, Yamada Y, Harasawa H, Tsuji T, Murata K, Sugahara K, Tsuruda K, Masuda M, Takasu N, Kamihira S: Restricted expression of tumor necrosis factor-related apoptosis-inducing ligand receptor 4 in human peripheral blood lymphocytes.
Cell Immunol 2004, 231(1-2):1-7. PubMed Abstract | Publisher Full Text
Lim SY, Raftery MJ, Goyette J, Hsu K, Geczy CL: Oxidative modifications of S100 proteins: functional regulation by redox.
J Leukoc Biol 2009. PubMed Abstract | Publisher Full Text
Yamasaki S, Ishikawa E, Sakuma M, Hara H, Ogata K, Saito T: Mincle is an ITAM-coupled activating receptor that senses damaged cells.
Nat Immunol 2008, 9(10):1179-1188. PubMed Abstract | Publisher Full Text
Teixeira VH, Olaso R, Martin-Magniette ML, Lasbleiz S, Jacq L, Oliveira CR, Hilliquin P, Gut I, Cornelis F, Petit-Teixeira E: Transcriptome analysis describing new immunity and defense genes in peripheral blood mononuclear cells of rheumatoid arthritis patients.
PLoS One 2009, 4(8):e6803. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Chung CP, Oeser A, Raggi P, Gebretsadik T, Shintani AK, Sokka T, Pincus T, Avalos I, Stein CM: Increased coronary-artery atherosclerosis in rheumatoid arthritis: relationship to disease duration and cardiovascular risk factors.
Arthritis Rheum 2005, 52(10):3045-3053. PubMed Abstract | Publisher Full Text
Cruz-Munoz ME, Dong Z, Shi X, Zhang S, Veillette A: Influence of CRACC, a SLAM family receptor coupled to the adaptor EAT-2, on natural killer cell function.
Nat Immunol 2009, 10(3):297-305. PubMed Abstract | Publisher Full Text
Kim DK, Kabat J, Borrego F, Sanni TB, You CH, Coligan JE: Human NKG2F is expressed and can associate with DAP12.
Mol Immunol 2004, 41(1):53-62. PubMed Abstract | Publisher Full Text
Whitman SC, Rateri DL, Szilvassy SJ, Yokoyama W, Daugherty A: Depletion of natural killer cell function decreases atherosclerosis in low-density lipoprotein receptor null mice.
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Robertson AK, Hansson GK: T cells in atherogenesis: for better or for worse?
Arterioscler Thromb Vasc Biol 2006, 26(11):2421-2432. PubMed Abstract | Publisher Full Text
Ait-Oufella H, Herbin O, Bouaziz JD, Binder CJ, Uyttenhove C, Laurans L, Taleb S, Van Vre E, Esposito B, Vilar J, et al.: B cell depletion reduces the development of atherosclerosis in mice.
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Zou H, Hastie T: Regularization and variable selection via the elastic net.
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If you haven't changed your deodorant since adolescence, consider swapping in this next-generation antiperspirant. The invisible solid stick features an FDA-approved blend of aluminum zirconium tirchlorohydreax to keep you dry, aloe and vitamin E to soften and nourish skin, and sunflower and castor oils to prevent irritation. A clean, slightly citrusy scent controls odor without being overbearing.


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Delivery update on routes delayed due to storm damageJuly 09,2014Delivery update: Three routes are delayed by 2 hours this morning, in Danby, Wallingford, and Mount Holly due to storm damage. The carrier was delayed by a fallen tree. Thank you for your patience! - RHMORE IN This Just InKABUL, Afghanistan Confusion reigned in the wake of the deadly bombing Saturday of a hospital... Full StorySPRINGFIELD A fast-moving fire destroyed a freestanding two-bay garage on 432 South St. Full StoryIt will be a busy week in gubernatorial politics with two candidates holding formal campaign... Full Story
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Serving the Cambridge community since 1922, the Rotary Club of Cambridge is a mix of business people, entrepreneurs and professionals making connections whilst working for local and international community projects.
Want to know more? Do you want to learn about joining? Please press the "How to contact us" tab above.
Scroll down for the advantages of Rotary membership.
Cambridge News reports:
Doctor's notes just the job in talk to Rotarians
One of the Rotary Club's newest members has given a fascinating talk about his job.
Jo Chan, who qualified as a doctor in Manchester in 1979, worked in hospitals in the North West.
His talk revealed the pitfalls and successes of his profession, time being spent in frantic and life saving activity and also periods of boredom and hopefully, rest.
Members heard that in 1984, he joined the Clinical Research Centre in Harrow, later specialising in molecular genetics at the Institute of Child Health. One example of the dangers of complacency and jumping to conclusions was a man who appeared dead on arrival and had no apparent pulse was in fact alive and survived. Things are not always what they seem to be. He told members that conditions that appear dire may have a silver lining, and symptoms that seem trivial may not be. Since retiring in 2013, Jo has studied Roman History at U3A.
you to the Cambridge News August 22, 2015.
Another new member joins us
Rotarian Peter Pugh has been inducted by president Graham Sanderson, seen here in the gardens at the Gonville Hotel, Cambridge.
Peter was educated at Oundle School and Churchill College where he read History. For the past 30 years he has been writing over 50 company histories including a three volume History of Rolls Royce. He is currently working on volumes on Papworth Hospital and Domino Printing Sciences.
He also founded Icon Books, a publishing company, 25 years ago.
He is a keen tennis player and is married to Felicity with three sons and seven grandchildren.
|Advantages of Rotary Membership
Rotary is a membership organisation open to men and women.
We like to ensure that the membership of our Club is as representative as possible of the Cambridge business and professional community.
It provides the opportunity for busy people to get to know a broad spectrum of local people who perhaps they would not otherwise meet.
Links: Rotary International
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FactSet Consultant Interview Questions
Interview Experience
Interview Experience
Getting an Interview
Getting an Interview
Interview Difficulty
Interviews for Top Jobs at FactSet
- Consultant (190)
- Software Engineer (138)
- Graduate Consultant (32)
- Research Analyst (20)
- Financial Consultant (11)
- Senior Software Engineer (9)
- Financial Engineer (8)
- Software Engineer I (8)
- Software Developer (6)
- Product Developer (5)
- Research Associate (4)
- Equity Research Analyst (4)
- Research Specialist (4)
- Quality Assurance Analyst (4)
- Systems Engineer (3)
- Database Administrator (3)
- Software Engineer Intern (3)
- Associate Content Manager (2)
- Intern (2)
- Product Specialist (2)
- Team Leader (2)
- Sales Specialist (2)
- Senior Consultant (2)
- QA Analyst (2)
- Content Analyst (2)
- Content Manager (1)
- Content Specialist (1)
- QA Associate (1)
- QA Automation (1)
- QA Automation Engineer (1)
191 Candidate Interview Reviews Back to all InterviewsSort: Popular Date Difficulty
Consultant Interview
I applied through college or university. I interviewed at FactSet.
I interviewed on campus and got called back for the final round in their office. I will be going to the final round in a couple of weeks. The interviewer was friendly and gave plenty of time for questions at the end. It took about half an hour, maybe a little bit over.
Interview Questions
- What is one thing that interests you about consulting? What is your interest in finance? Answer Question
Other Interview Reviews for FactSet
Helpful (10)
Consultant InterviewAccepted OfferNegative ExperienceAverage Interview
I applied through an employee referral. I interviewed at FactSet.
- Phone Interview: Pretty easy questions like what do you know about FactSet. What news providers do you read? - In person interview: You meet with about 5 different people who work at FactSet. All asking you the same questions that are listed on Glassdoor. If you do your research and be prepared you will do great.
Interview Questions
- Walk me through an app on your phone. Answer Question
Helpful (3)
Consultant InterviewAccepted OfferPositive ExperienceAverage Interview
I applied through college or university. The process took a week. I interviewed at FactSet (Norwalk, CT) in July 2015.
Overall experience was great! Had 5 interviews then lunch and two 5 minute quizzes. The interviewers were extremely friendly and seemed genuinely interested in learning more about your background and why you are a great fit for the job. There weren't any difficult questions asked if you had prepared by going through the previous questions listed on glassdoor. I recommend reviewing basic functions in excel (if statements, vlookups, pivot tables) before the interview.
Interview Questions
- Why did you choose your school? Why did you choose your major? Tell me about news going on in the financial world What stock would you recommend investing in? Why FactSet? What do you know about FactSet? Why consulting? If someone asked you what FactSet does and what your job is there, what would you tell them? Who are FactSet's competitors? What do you like to do in your free time? Tell me about a time you broke the rules for a good reason What qualities do you think a consultant should have? What would your last manager say about you? What news providers do you follow? Answer Question
Helpful (7)
Consultant InterviewNo OfferPositive ExperienceAverage Interview
I applied through an employee referral. I interviewed at FactSet in June 2015.
I have a family member who works for the company. They referred me for the position and I got a call from a recruiter who then set up a phone interview during the next week.
Interview Questions
- What has your job search been like? Why did you choose your school? Answer Question
Helpful (2)
Consultant InterviewNo OfferNeutral ExperienceDifficult Interview
I applied through an employee referral. The process took 2 weeks. I interviewed at FactSet (New York, NY) in June 2015.
Phone interview, then on site. Nice people, and they get back to you very quickly. Came in and gave a presentation about the software, and then was sent to 3 different interviews where they asked personality questions and did some excel stuff.
Helpful (1)
Consultant InterviewNo OfferNeutral ExperienceAverage Interview
I applied through college or university. I interviewed at FactSet.
I had a phone interview with a manager at FactSet who seemed thoughtful and courteous. He asked mostly standard interview questions about my knowledge of FactSet and my experience and approach towards communicating with clients, troubleshooting their needs, and suggesting additional solutions. He also told me about the company culture and what was expected of the role.
Interview Questions
- How would you describe what FactSet does? Answer Question
Consultant InterviewNo OfferPositive ExperienceAverage Interview
I applied through an employee referral. The process took 3 weeks. I interviewed at FactSet (Chicago, IL) in May 2015.
I was referred from a former employee which resulted in an initial phone interview. After that, I was scheduled for 4 interviews in their Chicago Office. Those 4 interviews consisted of talking with 2 consultants, the regional director, a consultant manager, and lastly two other consultant managers.
Interview Questions
- Rate yourself on a scale 1-10 in Excel, would then ask you to answer questions according to your answer. Answer Question
Helpful (12)
Consultant InterviewNo OfferNeutral ExperienceDifficult Interview
I applied through college or university. The process took 1+ week. I interviewed at FactSet in March 2015.
For the first round I had an on-campus interview. There was an info session the night before with everyone who had been granted an interview as well. After preparing through Glassdoor, I was ready for every question the interviewer had but I thought it went very badly since the interviewer hadn't smiled at all the entire time. Despite this, at the end of the interview, they asked me to come for a second round at their office. The second round was held with a couple of other candidates. Before the interviews, there was a short demo on the software itself. The demo didn't come into play during the interviews but only served as a kind of intro to the technology. Afterwards, we each had four 30min interviews. It was all behavioral and they even asked some overlapping questions but one session had an excel test and a brainteaser game (yes, it was a game and not just a question). They took us all out to lunch at the end. Some other reviewers stated that the interviewers didn't seem interested in what they were saying but I've noticed that it might be because FactSet employees are all incredibly calm since the company looks for people who deal well under stress and pressure.
Interview Questions
- What is your biggest pet peeve? What do you like to do in your free time? Pitch a recent article you read or piece of interest you have to me. Why FactSet? Why consulting? Why shouldn't I hire you? Talk about a time when you've gotten negative feedback/criticism by your boss. What do you look for in a job posting? Here's a list of numbers 1-5000, how find the one number that is missing using excel? Answer Question
Consultant InterviewNo OfferNegative ExperienceEasy Interview
I applied online. The process took 4+ weeks. I interviewed at FactSet (Boston, MA) in April 2015.
Day 1: Apply with resume and cover letter Day 2: Asked to phone interview. I replied with my availability. Day 10: Phone interview finally arranged for that day. Easy phone interview. Invited to interview in person Day 12: Interview along with other candidates. Rotate between four interviews after viewing a software demo. The demo did not work, and they blamed the internet connection. Two young consultants took us to lunch after the interviews. At this stage that I learned the key to acing a factset interview: have an employee referral. The consultants and candidates then spent the lunch discussing their referrals and what friends they had in common. The consultants had been referred themselves and they informed these candidates about the referral process and how well it works. I felt left out. Day 28: No response yet, fairly unprofessional.
Interview Questions
- Game: Two people take turns drawing X's on a whiteboard and the first person to draw the 11th X wins. Each player can only put down one, two or three X's and they let you choose who goes first. Excel: Numbers 1-5000 with one missing, find the missing number. Why FactSet? Why consulting? Strengths/weakness? Time you failed? Pet peeve? What do you expect to be the biggest challenge as a consultant? Also they made us complete 5-minute exams, one of them similar to math SAT 1 Answer
Consultant InterviewNo OfferPositive ExperienceAverage Interview
I applied through an employee referral. The process took 2 weeks. I interviewed at FactSet (New York, NY) in April 2015.
One phone interview, then an in person interview in their New York office. The interview consisted of 4 interviews, of which two were one on one and two were two on one interviews. They asked to walk them through my resume, there were some behavioral questions, as well as some brain teasers.
Interview Questions
- Why the finance industry? Answer Question
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Because better schools benefit everyone. We need to educate the next generation, so they are able to eventually find employment and pay taxes. Or you can look forward to more people on welfare or in prisons (and you won't like paying for that, either!)<quoted text>
For instance, several years ago, the city put up a levy for a vote to build new schools. I don't have any kids in school, why would I vote for new schools?
If these arguments don't persuade you, consider that a main determinant of property values are the quality of the school systems in that area. Would you like to make sure that your home holds its value?


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PeopleSoft HRMS Reporting
Make your company's PeopleSoft investment pay off! Create the accurate, insightful reports you need, first time, every time, with PeopleSoft HRMS Reporting-the only complete guide to PeopleSoft reporting.
PeopleSoft specialist Adam Bromwich presents authoritative guidance on the successful design of PeopleSoft HRMS databases and reports for superior performance, ease of use, and value. Learn how the PeopleSoft database is organized-functionally and technically; review its key tables; and understand how PeopleSoft's online capabilities are linked to the relational database beneath them.
Discover how you can:
Bromwich reveals the hidden decisions PeopleSoft HR developers must make. How does calculating a payroll affect paycheck data? When does an effective dated table cause major headaches? Where is the accurate data stored and which tables should be left alone? You'll find common routines for retrieving functional data, techniques for slashing costly debugging and maintenance, and much more.
PeopleSoft HRMS Reporting is the only independent guide to real-world PeopleSoft reporting. And it's based on the unmatched experience of one of the world's leading PeopleSoft experts.


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Edit hours
Legendary Food, Service, and Fun! At Texas Roadhouse, we do it all with one goal - to give your family a place to go for great food and good times at a fantastic price.
Food is good. Service is great, but they do not understand the purpose of Call Ahead Seating at this location.
Tasty blue Hawaiian, yum
Nice rolls; great steaks; free peanuts! (The chili was only OK.) Busy as all damn hell, too! I might call ahead for a reservation next time, but it was a solid and affordable dining experience.
The bread and butter
OMG! We love this place! Will drive from Oakland to Union City for the rolls
Steak is alright, not the best, but definitely not the worst. The bread here is a little too sweet for my liking. Friendly servers though
The bread and New York strip are as good as any fine dining steak house out there
I don't even really like steak like that but this place...THIS place is definitely the best streak you'll ever have for it cheap price. Get the bone-in-ribeye steak or the ribs! Fall off the bone!
The bone-in ribeye is great. Great steak for the price.
The bread here is the bomb!!! Knock you out good!
Large parties aren't allowed to split the check.
Use their call ahead reservation feature. Unless you love sitting and eating their complimentary peanuts while waiting
The Bone in Ribeye is the best!
They offer call ahead seating
Bone in Ribeye is the best! Great flavor and tender.
there are hella Afro-Americans up in here tonight.
The staff actually dances here
Call ahead, its slammed on the weekend until they close the doors.
Try the Grilled Pork Chops
Ribs = quintessential "fall off the bone" ribs. Among the best I've ever had outside of Arthur Bryant's BBQ in KC and Texas. The best!
Get the rib eye!
there are so many people here, you'd think they handing out cures for cancer. translation = call ahead or wait until you get covered in cobwebs.
Try the 10Oz Ribeye Steak And Grilled Shrimp
Baby back ribs to die for
Ribs are super tender! Bread is best when hot and fresh!
Try the 22oz Bone In Rancher's Rib Eye - #rib-eye #hella #delicious
you can make a meal on their bread. so good!!
Try the Full Slab Ribs
If you have a peanut allergy go somewhere else
Don't fill up on the amazing bread. Ask for some to go at the end
Fall off the bone ribs
Go easy on the free peanuts and awesome bread. They're addicting!
Baslangic olarak Rattlesnake Bites ..Hearty cut Sirloin muthis..Tabi ortam ve ambiyansa masalarda ikram edilen kovalar icinde fistik bir harika tabiki bira ile birlikte
Ribs are the bomb. The only place you can make a mess and its all good!!!!
Closed Early for Presidents Day, BASTARDS!
Food is good
Peanuts! Garlic bread! Ribs! Can't go wrong with those!! I live how soft and tender the ribs are and the garlic bread and peanuts u can refill as many times as u want!!:) (3 of 4 petals via Fondu) Read more
Simply excellent
This place is in fact, tits.
The Margaritas here are excellent! GRUBCRAWL.COM
The grill chicken is great! The chicken critters are also good and the country fried steak! This place is great and the customer service is great too! Love it!
Great rolls and even better butter for them!
What an incredible clusterfuck this place is!
Cinnamon rolls are the BOMB!! Great steak for reasonable price.. Yuummmyy in my tummy!!
Buy a burlap bag for $5.99 and get free refills for life, even if you don't buy anything!
Rib eye is fantastic. Grace and Bianca are the best bar tenders ever.
Probably not the best place to come if you have a severe peanut allergy! Peanuts on tables, floor, air, ceiling! Lol
Great food.
Get a reservation so you don't have to listen to that horrible country music while waiting for a table. The chili is mediocre but the rib-eye is the best I've ever had!
Their dinner rolls with the cinnamon butter are heaven and their ribs are to die for. They seriosuly fall off the bone! Their steak is also very good. Call ahead for reservations if you can.


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Stereotaxis Interview Questions
Interview Experience
Interview Experience
Getting an Interview
Getting an Interview
Interview Difficulty
Interviews for Top Jobs at Stereotaxis
- Sales Director (1)
- Software Project Manager (1)
- Project Manager (1)
- Business (1)
Candidate Interview ReviewsSort: Popular Date Difficulty
- Accepted OfferNeutral ExperienceAverage Interview
I applied through a recruiter. I interviewed at Stereotaxis.
Team, group interview... full day. No written test.
Interview Questions
- Who was my favorite historical figure? Answer Question
Not a lot of room for negotiation... more like take it or leave it.
- Accepted OfferNegative ExperienceAverage Interview
I applied through an employee referral. The process took 4+ weeks. I interviewed at Stereotaxis (Saint Louis, MO).
Don't do it. It just isn't worth it,
Interview Questions
- How do you plan to pull the company out of it's $500M deficit without any capital sales reps? How do you plan to grow your disposable business if you continue to shrink the size of your sales team in this area? Answer Question
Try for as much as you can without a signon bonus. And don't take granted stock shares. They aren't worth anything.
- No OfferPositive ExperienceAverage Interview
I applied online. The process took 2 weeks. I interviewed at Stereotaxis (Saint Louis, MO) in January 2011.
Had two interviews - interviewer was very polite and helpful throughout the process. Highly recommend
- Accepted OfferPositive ExperienceAverage Interview
I applied through a recruiter. The process took 1 day. I interviewed at Stereotaxis (Saint Louis, MO) in January 2008.
Hiring process was a 1/2 day of interviews with multiple departments. They were very helpful with the introductions to their areas of expertise and what this position entailed.
Interview Questions
- Was asked to write a piece of code on the fly by the Director or Engineering. Answer Question
Negotiation was straightforward and painless.
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- Filed Under
A 37-year-old woman drowned Saturday in Easley while tubing with three other people, according to the Pickens County Coroner's Office.
The accident occurred at 3:33 p.m. Saturday at 1307 Old Easley Highway, according to a release from Pickens County Coroner Kandy Kelley.
The victim was identified as Heather Dobbins of 1462 South Pratian Road in York. She was transported to Greenville Hospital System where she was pronounced dead, ...


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Dropping in May is the Jordan Prime 5 in a Chicago Bulls-esque Black/Varsity Red-Metallic Silver colorway, marking the most 'everyman' release of the style thus far. Exact release date is yet to be confirmed.
Jordan 5 Prime 'Bulls'
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I spent 8 nights at this hotel for a dog show at Purina Farms (Yes some shows last that long!).
The hotel does need some updating but the staff really worked as hard or harder than any staff I have seen.
The Good:
The Staff - When I checked in, my room was not ready but the front desk said to leave them a cell number and they would call in 45-60 minutes. After 45 minutes I got a call saying my room was ready. That impressed me. Another example - our shower had problems and the staff worked until they fixed it and solved the problem.
The Restaurant - The portions were massive - Breakfast for one can easily feed two. Again, another friendly staff.
Pets - They take pets and the staff works hard to keep the property clean and provide bags for clean-up.
Lots of "Drive-Up" rooms.
Hotel takes care of their Rewards Club members.
The Bad (with apologies to the management);
Room Condition - These are old rooms and they REALLY need to be updated. The furnishings are dated and many of the Air Conditioners need to be serviced. The carpets need to be replaced - now.
Restaurant Policy needs help. At dinner we wanted a sandwich but that required you to go to the bar. It's not a good policy that the bar menu, room service, pick-up and restaurant menus vary and the wait staff cannot vary from that. I told the front desk manager about this at checkout and she was a bit - inflexible about this. (ouch!)
The Facility needs a major update - this is separate from the rooms. The old "Holidome" is just about the same as it was many years ago.
I varied on 3-4 stars for this property but in the end, the condition of the rooms drove me to the lower rating.
- Official Description (provided by the hotel):
- The Holiday-Inn at Six Flags is located a short walk from the main gate of the Six Flags St. Louis amusement park. In addition, the hotel offers an indoor Fun Dome that includes a pool, sauna, whirlpool, exercise area, video game area, putting green and shuffleboard. Walk to Six Flags St. Louis on a connecting sidewalk or enjoy a complimentary air-conditioned shuttle bus to and from the amusement park. Just minutes away is Hidden Valley Ski Resort, Jellystone Park, and Purina Farms. Kids Eat Free at all meals in our Haymarket Restaurant. Drive to any major St. Louis attraction all within 30 minutes, including the Gateway Arch, Anheuser Busch Brewery, St. Louis Zoo, Grants Farm, Cardinal Baseball/Busch Stadium, Rams Football/Edward Jones Dome, and Meramec Caverns. Enjoy an Unparalleled Environment at Holiday Inn Six Flags, constructed by Irish stone masons with handcrafted limestone walls, detailed woodwork and decorative brickwork provide a backdrop of warmth and comfort. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with IHG, Booking.com, Expedia, Travelocity, Priceline, Odigeo, Orbitz and Hotels.com so you can book your Holiday Inn Saint Louis West Six Flags reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Holiday Inn Saint Louis West Six Flags Hotel Eureka
- Holiday Inn Saint Louis West 6 Flags
- Holiday Inn Eureka
- Eureka Holiday Inn