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Los Angeles Clippers v Brooklyn Nets
Gerald Wallace and Brook Lopez of the Brooklyn Nets combine to stop... News PhotoBarclays Center - Brooklyn,Basketball - Sport,Borough - District Type,Brook Lopez,Brooklyn - New York,Brooklyn Nets - Basketball Team,Full Length,Gerald Wallace,Jamal Crawford,Los Angeles Clippers,NBA,NBA Pro Basketball,New York City,Sport,USA,VerticalPhotographer Bruce BennettCollection: Getty Images Sport 2012 Getty ImagesNEW YORK, NY - NOVEMBER 23: Gerald Wallace #45 and Brook Lopez #11 of the Brooklyn Nets combine to stop Jamal Crawford #11 of the Los Angeles Clippers at the Barclays Center on November 23, 2012 in the Brooklyn borough of New York City. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and/or using this photograph, user is consenting to the terms and conditions of the Getty Images License Agreement. The Nets defeated the Clippers 86-76. (Photo by Bruce Bennett/Getty Images)


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Record: 1-5-1, Finished 16th in APFA
APFA Season Summary
Scored 19 points (2.7/g), 17th of 21 in the APFA.
Allowed 109 points (15.6/g), 17th.
Differential of -90 points (-12.9/g), 15th.
Expected W-L: 0.1-6.9.
Coached by
Billy Marshall
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subset: commoncrawl


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Age of Wushu
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Real Estate Management and Development
Company Overview of 3-D Disaster Services, Inc.
Company Overview
3-D Disaster Services, Inc. operates as a full service disaster recovery company and offers temporary housing facilities and services. The company was incorporated in 2004 and is based in Fairhope, Alabama.
612 Nichols Avenue
Fairhope, AL 36532
United States
Founded in 2004
Key Executives for 3-D Disaster Services, Inc.
3-D Disaster Services, Inc. does not have any Key Executives recorded.
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See the rest of the story here .
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Western Union retreats after disclosing money laundering probe
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Humidity: 55%
Wind: 16 mph
January 16, 2014 8:14 am
A suspected kitchen mishap gutted an elderly man's home on Road 9 Wednesday right around lunch time.
January 14, 2014 8:43 am
Shelter manager: Start new year with a new pet
Kindness reigns at City of Powell/Moyer Animal Shelter despite occasional setbacks for the four-legged clients.
January 14, 2014 8:41 am
The Powell High School boys swim team took second in Friday and Saturday meets with some of the guys seizing first in events.
January 09, 2014 9:25 am
United Airlines summer flights are conceivable
United Airlines has offered to make some flights from Cody's airport to Chicago's O'Hare International Airport and back this summer.
January 03, 2014 8:31 am
State had allocated 26 could be harvested in designated areas
The quota of wolves that could be killed in Wyoming's wolf trophy areas was almost reached in 2013.
December 24, 2013 9:05 am
Dropping price of sugar and lower beet sugar content mean less money for farmers
Local Western Sugar Co-op beet farmers may take a beating this year.
December 19, 2013 8:58 am
Host Lander eased past the Powell High School boys in the swim meet Friday.
December 19, 2013 8:48 am
December 12, 2013 8:42 am
November 29, 2013 8:28 am
Get all the latest Powell news by subscribing to the Powell Tribune today!
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Taiwan handset industry overview - Jun 2008
Last update: Wednesday 9 July 2008
The use of handsets continues to grow around the world, both in mature and emerging markets, as the communication devices become ever more ingrained in people's everyday lives. Global...
Full report [news subscription required]Table of contents
Chart 1: Taiwan handset shipments trend and global market share, 2005-2010 (million units)
Table 2: Taiwan handset makers' business models and client types
Table 3: Top-5 mobile-handset brand rankings by shipments, 1Q08 (k units)
Taiwan optical disc drive overview - Jul 2007
Last update: Tuesday 14 August 2007
The year 2006 saw further consolidation in Taiwan's optical disc drive (ODD) industry, as the biggest player, Lite-On IT, took over the ODD manufacturing business of its closest...
Full report [news subscription required]Table of contents
Chart 1: Taiwan and worldwide ODD shipments, 2004-2009 (k units)
Table 2: Worldwide PC shipment growth (percentage growth in unit shipments)
Table 3: New products from second-tier Taiwan-based optical disc makers
Taiwan digital camera overview - Jun 2007
Last update: Monday 16 July 2007
Taiwan-based digital camera makers maintained a mild growth in 2006. The top-4 Taiwan-based makers, Foxconn (into which Premier Technology merged), Altek, Ability Enterprise and Asia...
Full report [news subscription required]Table of contents
Chart 1: Taiwan and global digital camera shipments 2004-2009 (k units)
Table 1: Top Taiwan digital camera maker shipments (million units)
CCD sensors continue to account for a major portion of Taiwan output
Chart 3: Quarterly shipments of CCD and CMOS digital cameras from Taiwan-based makers (k units)
Chart 4: Overall digital camera shipments from Taiwan broken down by resolution
Chart 5: CCD-based digital camera shipments from Taiwan broken down by resolution
Chart 6: CMOS-based digital camera shipments from Taiwan broken down by resolution
Chart 7: Taiwan CCD-based shipments by major makers, 4Q05-1Q07
Table 3: Annual company revenues for Taiwan-based digital camera manufacturers (NT$m)
Large-size LCD panel overview - Jun 2007
Last update: Friday 6 July 2007
Global large-size LCD panel market revenues are expected to increase at a compound annual growth rate (CAGR) of 12.5% to US$96.2 billion by 2011, up from US$53.5 billion in 2006,...
Full report [news subscription required]Table of contents
Chart 1: Global large-size panel shipments by application, 2006-2010 (m units)
Chart 4: Price trend for 15.4"W WXGA notebook panels, 2006 (US$)
Table 2: TFT LCD generation vs. panel cutting, 4G and more mature (mm/inch)
Table 3: TFT LCD generation vs. panel cutting, 5G and more advanced (mm/inch)
Table 5: First-tier global TFT LCD makers, capex plans in 2006 and 2007 (US$b)
Table 6: Capacity increase from new lines, 1Q06-4Q07 (k substrates)
Table 9: Taiwan TFT LCD panel makers, panel launch plans, 2007
Innolux boosts position in small- to medium-size panel market with Jemitek acquisition
Table 10: Taiwan major large-size panel makers, capex plans, 2002-2007 (NT$b)
Chart 5: Taiwan large-size panel makers, monthly consolidated sales (NT$m)
Chart 6: Taiwan large-size panel makers, monthly shipments (k units)
Targeting niche markets: Q&A with ST Liu, president of Acer handset unit
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text:
The 24-year-old striker has joined the Italian giants on a season-long loan from Arsenal.
Bendtner spent last season on loan at Sunderland.
Bendtner tweets: "I can now finally say I am a Juventus player. Very proud and can't wait to wear the stripes! I hope we will have great times together.
"I want to fulfil my potential here and show the fans my absolute best as that is yet to come. I am sure I will.
"Thanks for all the support from the Arsenal fans and the Sunderland fans, means the world. We always believe and that gets us far!"
Sporting director, Beppe Marotta told Sky Sport Italia: "Bendtner is already in Italy and will undergo a medical.
"Until the final paperwork is concluded, I will not say that the player is ours.
"He has talent and ability. Potentially a great player, one that has almost never been able to prove it."
More about...
What is your opinion? Feel free to discuss the article under. You have to comment by using your Facebook profile. Be fair and have respect for other people and opinions!
Who of Europe's top strikers will move to England this summer?
After a dreadful start to their Bundesliga campaign, Borussia Dortmund appear to have weathered the storm after going five matches without defeat.


text:
Properties in over 36 countries in 11 languages
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In 1953, Thomas Hecker Sr. bought a small real estate office from Ben Carpenter. The little upright log building was located next to what is now Hardy's Insurance and was named Tom Hecker Real Estate. By 1959, business was booming and Tom Sr built a new office at the corner of First and Fairlane right next to the fairgrounds and across from the state park. Sadly, Tom Hecker Sr died suddenly in the fall of 1959 at the young age of 52. His wife Bernadine and son Tom Hecker got their licenses and jumped in to keep the business going. In the interim, Bob Burns Sr stepped in as Broker to help the family out until Bernadine received her Broker's license a couple of years later.Bernadine (Hughes) Hecker was daughter of Elmer Hughes who with his twin brother Ellis Hughes and Fred Weatherhead were some of the first developers in Harrison as noted by the Hughes -Weatherhead Subdivisons in the City. Carrying on the family tradition of developing the land in and around Harrison, were Bernadine, her new husband, Leo Bischoff and Tom Hecker. You may recognize some of these areas that were created by the owners of Harrison Realty: Hiram Hills at Arnold Lake, Paradise Bend 1 & 2, Land O' Lakes, Little Long Lake 1-7, Whispering Pines and Birch Hills Sportsman's Club 1,2, & 3.The company name was changed to HARRISON REALTY in 1970 and incorporated in 1986 as it remains today, and is still family owned and operated. Tom Hecker took over the business as Leo and Bernadine gradually spent more time in Florida and retired. The original building was added on to and expanded in 1980. In 1981, sister Janet (Hecker) Dunkle got her license and came in full time as Associate Broker in 1994. Tom's wife, Barbara Hecker also was licensed in the early 80's and finally left the Mortgage business in 1997 to work full time as Associate Broker. Tom & Barb's son, Mark McCrary (1998) and his wife Andrea and in 2003 daughter, Kari (Hecker)Garber and her husband Carey. The sales team also includes Rose Mayfield, John Carlstrom, Denise Foster, Mary Kay Chisholm, Scott Willer and Ralph Szydlowski all of whom are valued members of our extended family here at Harrison Realty, IncIn 2009 Harrison Realty, Inc opened a full-service real estate office at 229 N Lake George Ave, Lake George, to better serve the needs of area residents and those wanting to purchase in the Lake George, Farwell and Lake areas.
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The 100 Best Movies Ever – The Bride of Frankenstein (#38)
The goddamn best.
This may end up being David Fincher's best movie.
Milkshakes excluded, this is still a monster.
DeNiro was a one of a kind in his prime.
So damn good.
Remember this?
Can you believe it's been FIFTEEN YEARS?
Remember Sammy Jankis!
Balls entering holes. And billiards!


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text:
|N·mero de publicaci¾n
|Tipo de publicaci¾n
|N·mero de solicitud
|Fecha de publicaci¾n
|14 Abr 2005
|Fecha de presentaci¾n
|3 Sep 2004
|Fecha de prioridad
|3 Sep 2003
|Tambiķn publicado como
|N·mero de publicaci¾n
|Ralph White
|Cesionario original
|White Ralph Richard
|Exportar cita
|BiBTeX, EndNote, RefMan
|Citas de patentes (14), Citada por (28), Clasificaciones (14)
|Enlaces externos: USPTO, Cesi¾n de USPTO, Espacenet
This application claims the benefit of U.S. provisional patent application Ser. No. 60/499,859, filed Sep. 3, 2003.
Current surgical techniques for anterior ligament replacement and/or stabilization are grouped into extracapsular procedures and intracapsular procedures. The extracapsular procedures use tissues or suture implants placed outside of the joint capsule in order to stabilize the joint. Heavy sutures (modified Flo imbrication), and the repositioning of the lateral collateral ligament (fibular head transposition), are the current accepted techniques in veterinary surgery.
Disclosed is a surgical procedure for extra capsular anterior ligament replacement or stabilization and a specialized surgical instrument therefor.
Intracapsular procedures require a graft from an adjacent tissue such as the straight patellar tendon or the fascia lata which is detached from its origin and inserted or repositioned through tunnels bored in the distal femur and/or proximal tibia. This surgery is done inside of the joint capsule, with both ends of the transplant being fixed to the walls of the tunnels and/or adjacent bone using techniques of the particular surgeon's choosing.
Instruments for determining the isometric points of an intracapsular graft attachment are well known in the art. U.S. Pat. Nos. 5,037,426 and 5,743,909 teach instruments that represent the current art for the intracapsular determination of isometric points of attachment of these grafts. However, the current extracapsnlar surgical procedures have not addressed the optimal placement of transplant tissue or suture at the isometric points. Accordingly, it is to be understood that the surgical procedure of this invention provides for the optimal placement of these tissues in an extracapsular procedure utilizing the surgical referencing instrument of this invention to identify the isometric points of attachment.
First, with regard to the surgical instrument 10 of this invention, there is shown in
As is evident in viewing
Block 20, here referred to as stationary block 20 may, as illustrated, be configured generally similar to the aforementioned adjustable block 18 with the exception that the mounting bore 28 extending through the block body is configured for free, rotatable reception on the non-threaded, reduced diameter shaft portion 16 of the rod member. As is readily apparent in viewing the drawings, this block member 20 is rotatably captured on the reduced diameter shaft portion 16 of the rod member between the abutting enlarged end 12′ of the threaded portion and a cotter pin 30 releasably engaged through a bore 32 provided through the shaft portion adjacent its outer terminal end. Also, the block member 20 includes a wire receiving bore 34 therethrough similar to the bore 24 described in connection with the adjustable block member 18. As seen in
From the foregoing it will be apparent that the instrument described thus far provides a tool whereby one referencing element, block member 20, is, rotatably secured in a stationary position adjacent the end of the elongated rod member, and a second referencing member, block member 18, is supported for adjustment toward and away from the first referencing member. In the particular embodiment illustrated, it will be apparent that, with block member 18 held against rotation, rotation of the rod, as by handle member 14, in different directions effectively threads the block member 18 correspondingly in opposite directions towards and away from the block member 20.
Having thus described the basic structure of a preferred embodiment of the surgical instrument of the present invention, the surgical procedure and the operation of the surgical instrument in the surgical procedure will now be described in connection with a procedure on the knee joint of a dog.
First, full scale or known scale latero-medial radiographs are taken of both knees in full flexed position and extended position. Using the latero-medial extended view, Gerdy's tubercle (GT) is identified and used as the tibial isometric reference point. This point is referenced on both radiographs with a marker. Using a standard compass, the center leg point is placed at GT. A first point is located antero-ventral to the lateral femoral fabella on the lateral femoral condyle, and the compass is adjusted to that distance. This process is repeated on the latero-medial flexed view. By repeating this process back and forth between radiographs of the joint in extended and flexed positions, making minor adjustments in the compass reference points, the optimal isometric point IP may be identified on the femur at the point at which the distance A between the finally identified point on the femur and the reference point GT is substantially identical on the radiographs of the joint with the knee in fully extended and flexed conditions as is indicated in
The isometric referencing instrument is adjusted so that the center of the bores 24 and 34 on the block members 18 and 20 equal the isometric distance A previously determined by the aforementioned compass method. The isometric referencing instrument is then sterilized for surgery.
An anterorlateral surgical approach along the lateral margin of the straight patellar tendon and vastus lateralis muscle is made to expose the tibial crest, lateral margin of the patellar tendon, patella and distal vastus lateralis. The lateral border of the straight patellar tendon, patella and lateral body of the vastus lateralis muscle are identified and carefully dissected free from the underlying joint capsule, lateral patella ligament, and adjacent biceps femoris muscle. This identifies the anterior border of the fascia lata and exposes the tendonous insertion of the fascia lata on the proximal tibia at Gerdy's tubercle (GT). A lateral or medeal arthrotomy is made to inspect the joint contents. Appropriate procedures are completed and the joint is flushed and closed.
A 1.5 mm K-wire 26 is inserted into the bore 24 through block 18 and directed perpendicularly to the long axis of the tibia into the tibial tuberosity at Gerdy's tubercle GT at the tendenous insertion of the fascia lata, being careful to avoid the joint space. The K-wire is then seated in conventional manner deep into the tibia once it has been determined that it is being placed accurately at Gerdy's tubercle GT. This temporary anchoring of the K-wire 26 at GT defines, by the anchored wire, the distal isometric reference point on the tibia located between the insertion of the straight patellar tendon and the muscular groove. The instrument is then positioned on the seated wire by positioning the bore 24 of the block member 18 over the exposed end of the wire and lowering the instrument slidingly down onto the wire. The knee is placed in extension and a blunted 2.5 mm ({fraction (3/32)}″) Steinmann Pin 26′ is inserted through the bore 34 in the block member 20.
Having already predetermined and preset the isometric distance A between the bores 24, 34 of the block members 18, 20 during the aforementioned procedure with the radiographs, the femoral isometric point IP is located on the lateral femoral condyle using a trial and error positioning of the knee joint between flexion and extension. When the precise isometric point is determined, the tension corresponding to the isometric distance A reference will be zero at maximum flexion and extension. Therefore, the identified IP point on the femur is the common point at which the Steinmann Pin contacts the femur when the leg is in each of its extended and flexed conditions with the surgical instrument supported on the seated K-wire anchored at Gerdy's tubercle GT.
The purpose, of course, of using a blunted Steinmann pin while ascertaining the IP point is to assure against tearing or damage to the soft tissues in the area particularly as the leg is moved between extension and flexion. With the IP point thus located, the blunted Steinmann pin is removed from the bore 34 and replaced with a shortened 2.5 mm (3/32″) Steinmann trocar tipped pin, which is driven into the lateral femoral condyle point at the isometric point IP to a depth of approximately 10 mm. The pin is left in place temporarily to mark the femoral isometric point. The referencing instrument is then removed from the Steinmann trocar tip pin and K-wire 26. The distal K-wire 26 may also be removed from its seated mount on the tibia at Gerdy's tubercle GT.
A second incision is begun at a point 5-15 mm caudal to the tendonous insertion of the fascia lata at Gerdy's tubercle GT. This incision is extended with blunt dissection along the fascial lines of the fascia lata and the caudal aspect of the vastus lateralis muscle to the isometric point IP on the femur previously referenced by the shortened Steinmann Pin. The incision is extended proximally for about 15 mm. The tissue transplant is freed from deeper structures by blunt dissection. The lateral patellar ligament and insertion of the long digital extensor tendon are preserved and the transplant is freed distally to the level of Gerdy's tubercle.
A periosteal H-plasty is performed at the Steinmann Pin located at IP. The cortical bone that underlies the H-plasty is roughened with an elevator to encourage new bone growth into the graft. A 2.7 mm bone anchor is threaded with two pieces of non-absorbable monofilament nylon sutureleaving four strands of suture. The Steinmann Pin is then removed and the 3.5 mm bone anchor is driven into the femoral condyle at the isometric point IP. One piece (2 strands) will be used to apply traction to the transplant and transfix the transplant to the bone anchor.
Once the bone anchor is seated and the H-plasty is performed with the flaps retracted with appropriate suture material, the tool of this invention may be used in a second capacity to apply traction to the transplant thusly: A stab incision is performed in a linear fashion over and proximal to the anchor in the fascia lata transplant. This allows the anchor and the strands of suture material to protrude through the incision and transplant. A strand of suture is passed through the holes (36) in the proximal block (20) and transfixed to the graft at a point approximately 15 mm distal to the bone anchor in place. The screw mechanism is adjusted such that block (20) moves away from the bone anchor, thus placing the transplant in tension. Once all joint instability is eliminated with this adjustment, the suture strands in place through the bone anchor are used to transfix the transplant and maintain the tension on the transplant. The "tension" suture is severed, releasing the block from the graft and the graft is tested for tension through range of motion. The joint is tested for stability. Once the joint is stable through range of motion, the retaining sutures on the H-plasty are used to transfix the fascial lata transplant in place.
This traction suture places the graft in moderate tension. At this point in the surgery, joint stability should be restored in full flexion and extension. The other piece of suture is likewise used to transfix the transplant immediately distal the bone anchor. Finally the flaps of the periosteal H-plasty are sutured in a transfixing manner to the graft such that the periosteal flaps wrap around the transplant and ensure that the transplant is secured to the periosteum and is in direct contact with cortical bone of the lateral femoral condyle.
A Flo-type suture may then be placed in the traditional manner and aligned with both strands parallel to the fascia lata transplant. The distal fixation bore holes on the distal straight patellar tendon and tibial crest are determined such that the suture remains parallel to the transplant throughout the entire range of motion 5 of the joint. This minimizes the chance for the Flo-type suture to interfere with, or injure the transplant.
The suture is tightened until joint instability is eliminated throughout the entire range of motion of the joint. The tensioned suture is tested for isometric correctness, ensuring that the tensioned suture has not affected the isometric distance previously determined. This suture acts as a bridge that protects the fascia lata transplant during the re-vascularization and strengthening of that suture. The suture will ultimately fail, leaving the isometric transplant to function as the extra capsular anterior cruciate ligament. The tissues are then finally closed in typical manner.
It will be apparent to those skilled in the art that various changes can be made in the procedure and techniques described hereinbefore with respect to the surgical procedure as may be desired or needed in different cases. Also, it will be apparent to those skilled in the art that various changes, other than those discussed hereinbefore may be made in the size, shape, type, number and arrangement of the parts and structures of the surgical instrument described hereinbefore without departing from the spirit of the invention.
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text:
Page 1
Copyright ? 2007 by the Genetics Society of America
DOI: 10.1534/genetics.106.065516
A Specific Subset of Transient Receptor Potential Vanilloid-Type Channel
Subunits in Caenorhabditis elegans Endocrine Cells Function as Mixed
Heteromers to Promote Neurotransmitter Release
Antony M. Jose,1I. Amy Bany,2Daniel L. Chase and Michael R. Koelle3
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520
Manuscript received September 5, 2006
Accepted for publication October 2, 2006
Transient receptor potential (TRP) channel subunits form homotetramers that function in sensory
transduction. Heteromeric channels also form, but their physiological subunit compositions and functions
are largely unknown. We found a dominant-negative mutant of the C. elegans TRPV (vanilloid-type) subunit
OCR-2 that apparently incorporates into and inactivates OCR-2 homomers as well as heteromers with the
TRPV subunits OCR-1 and -4, resulting in a premature egg-laying defect. This defect is reproduced by
knocking out all three OCR genes, but not by any single knockout. Thus a mixture of redundant
heteromeric channels prevents premature egg laying. These channels, as well as the G-protein Gao,
function in neuroendocrine cells to promote release of neurotransmitters that block egg laying until eggs
filling the uterus deform the neuroendocrine cells. The TRPV channel OSM-9, previously suggested to be
an obligate heteromeric partner of OCR-2 in sensory neurons, is expressed in the neuroendocrine cells but
has no detectable role in egg laying. Our results identify a specific set of heteromeric TRPV channels that
redundantly regulate neuroendocrine function and show that a subunit combination that functions in
sensory neurons is also present in neuroendocrine cells but has no detectable function in these cells.
transient receptor potential (TRP) family to translate
sensory stimuli into electrical signals (Montell 2005).
These tetrameric cation channels can be homomers of
identical subunits or heteromers of two or more dif-
ferent subunits. TRP channels have been widely studied
by overexpressing homomeric channels in cultured
cells or Xenopus oocytes. However, it remains unclear
to what extent native TRP channels function as homo-
mers vs. as heteromers, and what rules might govern
the association of the various TRP subunits into func-
tional heteromers.
Genetic studies can potentially reveal the physiolog-
ical functions of TRP channels and whether homomers
or heteromers carry out these functions. Four of the six
mammalian TRPV (vanilloid-type) subunits have been
knocked out in mice. TRPV1 knockouts have defects in
responding to noxious stimuli (Caterina et al. 2000;
Davis et al. 2000), in osmosensation by neurons of the
supraoptic nucleus (Naeini et al. 2006), and in mecha-
OUCH, hearing, taste, vision, smell, and temper-
ature sensation may all rely on channels of the
nosensation by urothelial cells (Birder et al. 2002).
TRPV3 knockouts have a defect in thermosensation by
the skin (Moqrich et al. 2005). TRPV4 knockouts have
defects in sensing systemic osmotic pressure (Liedtke
et al. 2000). Finally, TRPV5 knockouts have renal Ca21-
handling defects (Hoenderop et al. 2003a). It remains
unclear whether these defects are due to loss of ho-
momeric channels or due to the knockouts disrupting
a more complex mixture of heteromers. In addition,
TRPV subunits are expressed in overlapping patterns
with other TRP subunits in tissues such as the inner ear,
brain, and heart (Montell 2005), where their func-
tions have not been revealed by the knockout studies.
One reason for this could be that, as in Drosophila
vision, coexpressed TRP subunits may compensate
for the lack of one TRP subunit in single knockouts
(Niemeyer et al. 1996; Reuss et al. 1997).
The functions of coexpressed TRPV subunits have
invertebrates (Montell 2005; Kahn-Kirby and Barg-
mann 2006). The Caenorhabditis elegans TRPV subunit
OSM-9 is found in the ciliated endings of neurons that
sense touch to the nose (Colbert et al. 1997). Nose
TRPV subunit, OCR-2, which depend on each other for
localization to cilia (Tobinet al. 2002). The Drosophila
TRPV subunits IAV and NAN (similar to OSM-9 and
OCR-2, respectively) similarly depend on each other for
localization to chordotonal cilia (Kim et al. 2003; Gong
1Present address: Department of Molecular and Cellular Biology,
Harvard University, Cambridge, MA 02138.
2Present address: Office of the Director, National Institutes of Health,
Bethesda, MD 20892.
3Corresponding author: Department of Molecular Biophysics and Bio-
chemistry, Yale University School of Medicine, SHM CE30, New Haven,
CT 06520-8024. Email: email@example.com
Genetics 175: 93–105 ( January 2007)
Page 2
the hypothesis that the coexpressed OSM-9/IAV and
OCR-2/NAN subunits form obligate heteromers for
proper localization to cilia and for function.
Here, we find that three coexpressed C. elegans TRPV
subunits (OCR-1, -2, and -4) apparently form a complex
mixture of functionally redundant homomeric and
heteromeric TRPV channels to control neurotransmit-
ter release from neuroendocrine cells. These channels
can be inactivated by a dominant-negative OCR-2 sub-
unit to reveal a defect in egg-laying behavior not seen
in any of the single subunit knockouts. Further, the
obligate partner of OCR-2 in sensory neurons, OSM-9,
although coexpressed with the other channel subunits
in the neuroendocrine cells, does not function with
them in these cells.
C. elegans strains: All strains except those containing dpy-
20(e1282ts) were grown at 20? and maintained using standard
methods (Brenner 1974). Worms containing dpy-20(e1282ts)
were grown at 25?. The strains used in this study included N2
wild type, LX671 ocr-2(vs29) IV, LX843 ocr-2(ak47) IV, CX10
osm-9(ky10) IV, LX844 ocr-1(ok132) V, LX979 ocr-1(ak46) V,
LX950 ocr-4(vs137) IV, LX980 ocr-4(vs137) IV; ocr-1(ok132) V,
LX981 ocr-2(ak47) ocr-4(vs137) IV, LX982 ocr-2(ak47) ocr-
4(vs137) IV; ocr-1(ok132) V, LX842 ocr-2(vs29) osm-9(ky10) IV,
LX748 ocr-2(ak47) osm-9(ky10) IV, LX983 ocr-2(ak47) osm-
9(ky10) IV; ocr-1(ak46) V, LX984 ocr-4(vs137) osm-9(ky10) IV,
MT13113 tdc-1(n3419) II, LX845 ocr-2(ak47) IV; ocr-1(ok132) V,
LX669 unc-44(e362) ocr-2(vs29) dpy-20(e1282ts) IV, LX670 ocr-
2(vs29) dpy-20(e1282ts) IV, LX725 ocr-2(ak47) dpy-20(e1282ts)
IV, MT8189 lin-15(n765ts), and LX491 goa-1(n1134) I; lin-
15(n765ts) X.
ocr-4 deletion mutant: An ocr-4 deletion (vs137) was gener-
ated using standard C. elegans gene knockout methods (Hess
et al. 2005). vs137 has a 688-bp deletion with 1 base inserted
(uppercase ''T'' below). The resulting sequence around the
Behavioral assays: Animals for behavioral assays were
isolated as late L4 larvae and aged 11.5 hr at 20? (Figure
1C),24hrat25?(Figure 2D),or 36hr at20?(allother figures)
to obtain precisely staged adults. Numbers of unlaid or
prematurely laid eggs were measured as in Chase and Koelle
(2004) or Jose and Koelle (2005), respectively. Nose touch
avoidance (Kaplan and Horvitz 1993) and osmotic avoid-
ance (Hilliard et al. 2002) assays were adapted as described
below. Worms were placed on agar plates with no bacteria and
assayed 10–40 sec later. For nose touch avoidance, an eyelash
was placed in the path of an advancing worm to cause head-on
collisions and a response (stopping forward movement and
starting a reversal within 3 sec) was scored. vs29 animals failed
vs29 animals attempted to move under it. For osmotic
avoidance, a 100-nl drop of 2 m fructose (Sigma, St. Louis)
was placed in the path of an advancing worm and a response
(stopping forward movement, starting a reversal within 3 sec,
briefly stopped forward movement and then advanced into
the drop, whereas vs29 animals briefly stopped forward
movement, initiated a short reversal, and then advanced into
the drop. For transgenic experiments, animals that showed
expression of the cotransformation marker were chosen from
five independent transgenic lines, and 40 eggs laid by animals
from each line were assayed. Student's t-test was used to
calculate 95% confidence intervals for numbers of eggs shown
in Figure 1, A–C. In all other assays, 95% confidence intervals
for a single proportion were calculated using Wilson's esti-
mates, and P-values for comparison of two proportions were
calculated using the proportion of pooled values (Moore and
McCabe 2003).
Transgenes: Germline transformation was as described by
Mello etal.(1991).Rescueoftheegg-layingdefectofvs29was
attempted using the cosmids C07G1 and T09A12 (i and ii,
respectively,insupplementalFigureS1athttp:/ /www.genetics.
org/supplemental/), and a plasmid (iii in supplemental
Figure S1 at http:/ /www.genetics.org/supplemental/) with
ocr-2 genomic DNAcontaining 2.4 kbupstream of theinitiator
ATG, the coding region, and 1.0 kb of downstream sequences.
A total of 10 ng/ml of each clone and 10 ng/ml of myo-2Tgfp
(co-injection marker, gift of A. Fire, Stanford University) was
injected into vs29 animals. For OCR-2 overexpression experi-
ments, cDNA encoding OCR-2 (gift from C. Bargmann,
Rockefeller University) or encoding OCR-2(Y395F) was used
to replace the ocr-2 coding region in supplemental Figure
10 ng/ml of myo-2Tgfp was injected into wild-type animals. To
express full-length OCR-2TGFP or OCR-2(Y395F)TGFP, the
gfp gene from pPD95.69 (gift of A. Fire) was fused to the wild-
type cDNA construct above or the OCR-2(Y395F) cDNA to
make fusion proteins with green fluorescent protein (GFP)
precisely after the C-terminal residue of OCR-2 or OCR-
2(Y395F), respectively. These were injected at 20 ng/ml with
50 ng/ml of the co-injection marker pL15EK (Moresco and
Koelle 2004) into MT8189 animals. For overexpression of C.
elegans TRPV genes, a genomic region for each was amplified
(GeneAmp XL, Applied Biosystems, Foster City, CA), contain-
ing coding regions along with 59 and 39 regulatory regions
extending to the neighboring genes. These were injected at
50 ng/ml with 10 ng/ml of myo-2Tgfp (co-injection marker)
into vs29 animals.
fusion constructs were made on the basis of the method of
Hobert (2002) as follows: 59 and 39 regulatory regions
(extending to the neighboring genes) were amplified and
fused byoverlapextension PCR tothegfp geneamplified from
pPD95.69. These were injected at 20 ng/ml along with the
coinjection marker pL15EK (50 ng/ml) into MT8189 animals.
We alsomadereporter constructs analogous tothose ofTobin
et al. (2002) lacking the 39 untranslated region (UTR) of ocr-2:
the 59 regulatory region or the 59 regulatory region plus the
coding region through the third exon was fused to gfp coding
sequences in pPD95.69 to make transcriptional (pAJ12) or
translational (pAJ11) reporters, respectively. These GFP con-
structs were coinjected with pL15EK (50 ng/ml) into MT8189
animals. Injection of pAJ11 and pAJ12 at 200 ng/ml labeled
the same cells as the PCR fusion products, but uv1 and a
number of other cells were much more strongly labeled by the
PCR fusion products injected at 20 ng/ml, apparently because
the PCR fusion products contained the ocr-2 39 UTR lacking in
pAJ11 and pAJ12.
The coding sequences of ocr-2 rescuing construct iii
(in supplemental Figure S1 at http:/ /www.genetics.org/
supplemental/) were replaced with coding sequences for the
S1 subunit of pertussis toxin PTx to inactivate Gaoor with the
release. A total of 10 ng/ml of toxin construct and 10 ng/ml
of pAJ12 (co-injection marker) was injected into wild-type
animals. Our TTx cDNA had a polymorphism that changes
94 A. M. Jose et al.
Page 3
Ile 361 to Val. Although this change is not expected to make
the toxin inactive, a reduction in its efficiency cannot be ruled
out. A transgene (pMK376) expressing functional GOA-
1TGFP was made based on the work of Hughes et al. (2001)
by inserting gfp coding sequences into goa-1 genomic DNA
such that GFP was inserted into an internal loop of the G
protein. Specifically, the sequences coding a flexible linker
(SGGGGS), full-length GFP, and another linker (SGGGTS)
were placed between goa-1 codons for T117and E118. pMK376
was injected at 5 ng/ml with 50 ng/ml pL15EK (co-injection
marker) into LX491 animals.
Sequence analysis: Multiplesequencealignmentsofhuman
and C. elegans TRPVs were performed using Clustal W and
Megalign (Lasergene). The accession numbers of the sequen-
Imaging: Confocal fluorescence images were obtained us-
ing an LSM-510 confocal microscope (Zeiss). We imaged two
worms that expressed the co-injection marker from each of
five independent transgenic lines expressing either OCR-2T
GFP or OCR-2(Y395F)TGFP (a total of 10 worms/transgene).
each animal was measured (ImageJ).
Mosaic analysis: The OCR-2TGFP construct (20 ng/ml) was
injected along with the co-injection marker pL15EK (50 ng/
ml) into ocr-2(vs29); lin-15(n765ts) animals to obtain a mosaic
transgenic line. To determine the cells in which OCR-2 func-
tions to control egg-laying behavior, rescue of the egg-laying
defect in ocr-2(vs29) was correlated with OCR-2TGFP expres-
sion (Figure 5). We counted the numberof eggs in the uteri of
hr at 20?. Presence or absence of OCR-2TGFP expression in
head neurons (AWA, ASH, ADL, ADF, and two unidentified
was scored as having OCR-2TGFP expression in that group.
More than 150 animals were screened for gfp expression to
obtain 10 animals to age at 20? for each mosaic pattern.
A mutation in the TRPV subunit OCR-2 causes
premature egg-laying behavior: Several neurotransmit-
et al. 2003; Moresco and Koelle 2004; Alkema et al.
2005). To identify molecules involved in such neuro-
transmission, we screened for mutants that exhibit
we present the analysis of one mutant, vs29, isolated in
this screen.
The uterus of a wild-type animal on average accumu-
lates 15 eggs due to a delay betweenegg production and
egg laying (Figure1A).Incontrast,vs29 mutant animals
on average accumulate only 7 eggs (Figure 1B). We
found that wild-type adults assayed shortly after the
larval-to-adult transition had made ?8 eggs/worm, but
retained all these eggsin the uterus, indicating that egg-
laying behavior had not yet begun (Figure 1C). vs29
also had made 8 eggs/worm, but had already laid 4 of
them (Figure 1C), indicating that egg-laying behavior
had begun too early in vs29 animals. Thus wild-type
animals inhibit egg laying until the uterus fills, while
vs29 animals lay eggs before the uterus fills and thus fail
to accumulate a normal number of eggs.
Fertilized C. elegans embryos (eggs) develop indepen-
dently of whether they are retained in the uterus or laid.
Since there is a delay between egg production and egg
laying, the eggs of wild-type animals develop in the
uterus such that all but 4% have progressed beyond the
eight-cell stage by the time they are laid (Figure 1D).
However, the eggs of vs29 animals are laid prematurely
such that 85% are at the eight-cell stage or earlier when
they are laid (Figure 1D). Thus, quantifying the per-
centage of early stage eggs laid provides a sensitive
measure of premature egg-laying behavior.
We mapped the vs29 mutation to an 80-kb region on
chromosome IV (supplemental Figure S1A at http:/ /
www.genetics.org/supplemental/). A multicopy trans-
gene containing only the wild-type TRPV subunit gene
ocr-2 from this region was able to rescue the premature
egg-laying defect of vs29 mutants (supplemental Figure
S1B at http:/ /www.genetics.org/supplemental/). OCR-
2, like all TRPV subunits, contains three ankyrin repeats
in its intracellular N terminus, followed by six trans-
membrane domains (Figure 1E; Tobin et al. 2002).
Sequencing the coding regions of the ocr-2 gene in vs29
revealed a point mutation that changes Tyr 395 to Phe.
This Tyr residue lies in a region conserved in all TRPV
subunits just N-terminal to the first transmembrane
not been studied. The Tyr residue mutated in vs29 is
conserved in most human and C. elegans TRPV subunits
(Figure 1F).
A dominant-negative activity has recently been dem-
onstrated for a minor splice variant of murine TRPV1
that lacks 10 amino acids (thick horizontal solid line,
Figure 1F), including the tyrosine corresponding to
Y395 of OCR-2. Coexpression of this minor splice
variant (TRPV1b) with the major TRPV1 isoform
(TRPV1a) in Xenopus oocytes strongly suppressed
capsaicin-evoked currents that were seen when the
major isoform was expressed alone (Wang et al. 2004).
Further, TRPV1b was unstable and functioned as a
dominant negative by heteromerizing with TRPV1a
and destabilizing as well as inactivating the heteromer.
These results suggest that OCR-2(Y395F) may function
as a dominant negative by a similar mechanism.
OCR-2(Y395F) is an unstable protein that causes
sensory defects seen in ocr-2 null mutants and an addi-
tional dominant premature egg-laying defect: To test
the hypothesis that OCR-2(Y395F) is an unstable pro-
tein that acts as a dominant-negative subunit by inacti-
vating heteromers, we expressed both Y395F mutant
andwild-type OCR-2subunits inXenopusoocytes.How-
ever, we failed to obtain current with wild-type OCR-2
Mixed Heteromeric TRPV Channels 95
Page 4
using several stimuli known to open other TRPV
channels (J. Chang, L. Heginbotham, A. M. Jose and
M. R. Koelle, unpublished results), as had been seen
previously by (Tobin et al. 2002), even upon coexpres-
sion of OSM-9, a subunit that functions with OCR-2 in
sensory neurons. Thus, OCR-2 may require an as-yet-
unknown gating stimulus or other components not
present in Xenopus oocytes. We therefore examined
native context.
To test if OCR-2(Y395F) is an unstable protein in vivo,
we generated transgenic animals expressing fusions of
full-length OCR-2 or OCR-2(Y395F) to GFP (Figure 2,
A and B). The transgene expressing OCR-2TGFP res-
cued the egg-laying defect seen in ocr-2(vs29) animals
[transgenic animals had 16 6 3 eggs held in utero vs. 7 6
0.6 eggs in nontransgenic ocr-2(vs29) animals]. We
examined five independent transgenic strains express-
proteins in tail sensory neurons, which had previously
been shown to localize OCR-2TGFP to the cell body
and to sensory cilia (Tobinet al. 2002). Consistent with
sensory neurons compared to OCR-2TGFP (12.1 6 8.1
vs. 46.3 6 8.2 fluorescence units/cell, P , 0.0002).
OCR-2TGFP was found in both the cell body and the
sensory cilia, but OCR-2(Y395F) could be detected only
in cell bodies.
To assess the function of OCR-2(Y395F) in vivo, we
compared the behavioral defects in ocr-2(vs29) animals
and ocr-2 null mutant animals. The ocr-2 null mutants
have defects in multiple sensory behaviors, but have no
egg-laying defects (Tobin et al. 2002). In an assay of
mechanosensation, most wild-type animals reverse in
response to nose touch, but both ocr-2 null mutants and
of osmosensation, most wild-type animals moved away
from a drop of high osmolarity solution, but both ocr-2
from the drop (Table 1). Thus sensory defects of ocr-2
null mutants were also present in ocr-2(vs29) animals. In
contrast to the similarity of their sensory defects, ocr-
2(vs29) animals were distinct from ocr-2 null mutants in
their egg-laying behavior. While ocr-2(vs29) animals
showed premature egg-laying behavior (laid 85% early
stage eggs), ocr-2 null mutants, like wild-type animals,
laid very few early stage eggs (Table 1). The fact that
Figure 1.—A missense mutation in the TRPV subunit OCR-2 causes eggs to be laid before the uterus is full. (A and B) vs29
animals accumulate fewer eggs than do wild-type animals. Representative wild-type (A) and vs29 (B) animals are shown. The av-
erage number of eggs accumulated by each strain, the vulva (asterisk), and eggs (arrowheads) are indicated. (C) vs29 animals
begin to lay eggs earlier than do wild-type animals. Animals isolated at the late L4 stage were aged 11.5 hr. The total eggs produced
during this time, the number laid, and the number retained in the uterus (unlaid) were counted. Averages for 10 animals/ge-
notype are shown. Error bars indicate a 95% confidence interval of the mean. A 0 on the x-axis indicates that no laid eggs were seen
for the wild type. (D) vs29 animals lay premature eggs. The developmental stages of freshly laid eggs (100/genotype) were de-
termined. The percentage of eggs not yet developed beyond the eight-cell stage (early stage eggs) is indicated. Error bars indicate
95% confidence intervals. (E) TRPV channel subunit schematic. Functional channels are tetramers of such subunits. Ankyrin
repeats (ovals), conserved region containing the vs29 mutation (open rectangle), transmembrane domains (solid rectangles),
and pore (P)-loop are indicated. (F) vs29 mutation is in a region conserved in C. elegans and human TRPVs. Multiple sequence
alignment of this region from C. elegans TRPV subunits (OCR-1–OCR-4 and OSM-9) and from human TRPV1–TRPV6. Amino acid
residues identical in more than six or in five to six subunits are solid or shaded, respectively. The sequence change in vs29 is
indicated and lies within a region (horizontal solid line) that is missing in the murine dominant-negative subunit TRPV1b (Wang
et al. 2004).
96 A. M. Jose et al.
Page 5
ocr-2(vs29) animals have a premature egg-laying defect
not seen in the ocr-2 null mutant suggests that OCR-
2(Y395F) does not simply lack all function, but rather
actively causes the premature egg-laying defect.
To test if OCR-2(Y395F) acts dominantly to cause the
egg-laying defect, we transgenically overexpressed wild-
type OCR-2 or OCR-2(Y395F) in wild-type animals
(Figure 2C). Whereas the wild-type protein had little
laying defect. Since some mechanosensitive channels
(Chalfie and Wolinsky 1990) and TRP channels
(Yoonet al. 2000) can be mutated to generate constitu-
tively open channels that cause neurodegeneration, the
observed effect of OCR-2(Y395F) may be caused by
degeneration of cells required to control egg laying. We
examined the morphology of cells that express OCR-2
by differential interference and fluorescence micros-
copy after labeling all of these cells with GFP or after
labeling a subset with the vital dye DiO (data not
shown). We saw no defects in these cells, indicating
that no degeneration had occurred. To further in-
vestigate how the vs29 mutation affected ocr-2 function,
we looked at the effects of varying the number of vs29
mutant and wild-type copies of the ocr-2 gene. We found
that reducing the number of copies of vs29 from two to
one reduced the severity of premature egg laying
(compare vs29/vs29 vs. vs29/ak47, Figure 2D), further
demonstrating that vs29 actively causes premature egg
laying in a dosage-dependent manner. Furthermore,
one copy of wild-type ocr-2 significantly suppressed the
defect caused by one copy of vs29 (compare vs29/ak47
vs. vs29/1, Figure 2D). This suggests that wild-type
OCR-2 subunits compete with the OCR-2(Y395F) sub-
units produced by ocr-2(vs29) to prevent the mutant
subunits from causing premature egg laying. The fact
that a single wild-type copy of ocr-2 outcompetes the
dominant-negative ocr-2(vs29) to cause recessive inher-
itance (compare vs29/1 vs. 1/1, Figure 2D) may be
due to the fact that the vs29 mutation produces an
unstable protein.
The above results show that OCR-2(Y395F) lacks
normal OCR-2 function, but actively causes premature
egg laying in a dosage-dependent manner, and that this
active function can be suppressed by wild-type OCR-2.
Since TRP subunits form homo- and heterotetramers
(Xu et al. 2000; Kedei et al. 2001; Tobin et al. 2002;
Hoenderop et al. 2003b; Strubing et al. 2003; Chuba-
nov et al. 2004; Gong et al. 2004), one model consistent
with all our results is that incorporation of a OCR-
2(Y395F) subunit makes a TRP tetramer nonfunctional
subunits allows functional tetramers that exclude Y395F
subunits to form. Using this model, the premature egg
laying of the vs29 mutant can be explained if the OCR-
2(Y395F) subunit, in addition to producing nonfunc-
tional OCR-2 homomers, also incorporates into and
makes nonfunctional tetramers containing other TRP
subunits that regulate egg laying.
Multiple TRP subunits, including OCR-1, OCR-2,
and OCR-4, control egg-laying behavior together: For
an in vivo test of our model that OCR-2(Y395F) makes
TRP heterotetramers nonfunctional, we reasoned that
Figure 2.—OCR-2(Y395F)isanunstabledominant-negative
subunit whose effects are suppressed by wild-type OCR-2. (A
and B) Expression of OCR-2TGFP and OCR-2(Y395F)TGFP.
Fluorescence images showing expression of full-length OCR-
2TGFP (A) and full-length OCR-2(Y395F)TGFP (B) in cell
bodies (brackets) and cilia (arrowheads) of sensory neurons
in the tail. OCR-2(Y395F)TGFP is an unstable protein. Bars,
30 mm. (C) OCR-2(Y395F) dominantly causes premature
egg-laying behavior. Wild-type OCR-2 or OCR-2(Y395F) was
transgenically overexpressed under the control of the ocr-2
promoter and the 39 regulatory region in a wild-type back-
ground. (D) Effect of varying dosage of ocr-2 alleles on egg lay-
ing. Animals carrying various combinations of ocr-2 alleles
were assayed for premature egg laying. 1, ak47, and vs29 de-
note wild-type, null, and vs29 alleles of ocr-2, respectively.
Brackets with asterisks indicate significant differences (P ,
0.05). Each strain analyzed was heterozygous for the marker
mutation dpy-20(1282ts), which was necessary to verify some
genotypes and was included in all others for consistency.
ocr-2(vs29) animals are like ocr-2(ak47) mutants in sensory
behaviors but not in egg-laying behavior
% response to:
GenotypeNose touch Fructose% early stage eggs
Wild type
90 6 6.1
22 6 8.1
24 6 8.3
88 6 6.0
10 6 6.1
24 6 8.3
4 6 4.5
3 6 4.1
85 6 7.1
Both ocr-2 null mutants [ocr-2(ak47)] and ocr-2(vs29) animals
were defective in mechanosensation (response to nose touch)
and in osmosensation (response to fructose). However, in
contrast to ocr-2 null mutants, ocr-2(vs29) animals additionally
laid a high percentage of early stage eggs. Errors indicate 95%
confidence interval.
Mixed Heteromeric TRPV Channels 97


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After School Club Leader / Party Entertainer
Here at Fun Science we love getting children excited about science through after school clubs and birthday parties. We are looking for someone to take on the running of a few after school clubs and birthday parties. This could be one person or two pe
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Post boxes, trash cans and lamp posts will chat up members of the public in what is perhaps the strangest public-art project to come out of the U.K.
Also, politicians in Naples are sick of you assuming they're all criminals, and the U.K. suspends the coolest teacher ever.
New York's latest foray into smaller living hits all the right notes.
Technology is poised to help us better share the roadway.
Man against machine.
Eduardo Kobra honors Oscar Niemeyer with a gigantic mural in Sao Paulo.
The summer melt in 2012 alone added a millimeter to the global sea level.
This is what it looks like when hundreds of thousands of people stand still at the exact same time.
No second bathrooms, no fireplaces or under-floor heating, no new balconies and no reserved parking places.
It's like a lamp store exploded in there!
The 114-degree heat in Sydney recently is nothing compared to 121-degrees recorded in Moomba.
"Sketching with a band saw" is how artist James McNabb describes his process for making his micro-lumber-cityscapes.
The South Ferry station is worth it — but that doesn't mean the money should be squandered.
Examining America's dramatic socio-economic residential segregation.
A month after a gruesome gang rape, New Delhi takes aim at the lowest-hanging fruit: Improving public spaces.
Recent polling data suggests the public is much less divided than many headlines would have you believe.
The municipal government can only do so much without support at the national level.
Fix it, or film it?
Next time you're in a hostage situation, remember that Apple phone's Compass app just may save your life.


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Westcoast Mountain Campers Ltd.
Ramada Plaza Calgary Airport Hotel & Conference Centre2.12km
At the Ramada Plaza Calgary Airport Hotel & Conference Centre a dynamic team of hospitality professionals are eager to show you their unique brand of hospitality.
Dalton's Restaurant at Ramada Plaza Calgary Airport Hotel & Conference Centre2.12km
Daltons Restaurant at Ramada Plaza Calgary Airport Hotel & Conference Centre serves breakfast, lunch, dinner (specialties: steak and seafood) and a weekend brunch buffet in a family-friendly atmosphere.
TELUS Spark, Calgary's Science Centre2.5km
Canada's first new purpose-built science centre in over 25 years.
Country Inn & Suites by Carlson Calgary Airport2.59km
Located between the foothills of the Canadian Rockies and rolling prairie flatlands, this Calgary hotel's accommodations feature convenient amenities.
Spark Event Management Inc.2.59km
Spark Event Management is a full service event management firm based in Calgary, Alberta that was created by pairing together our passion for business and...
Cactus Club Café Barlow NE2.66km
Proudly Canadian owned and operated, Cactus Club Cafe leads through innovation while remaining true to its Vancouver roots. Committed to delivering a unique...
Lakeview Signature Inn Calgary Airport2.7km
Residential living... hotel setting.
Calgary Zoo2.83km
One of North America's top zoos features African, Asian and South American wildlife, Canadian animals, large indoor botanical gardens and even dinosaurs.
ILLUMINASIA Lantern & Garden Festival2.83km
SEPTEMBER 17 to NOVEMBER 1, 2015. Calgary's first and only lantern and garden festival. This new culturally vibrant event will be the only one of its kind in Western Canada.
Bow Habitat Station2.99km
The Bow Habitat-Station is an eco-park with exhibits and interactive displays to teach students and families about Alberta's wetlands.
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Victory is 'Sweet,' with 8 Genie wins
"The Sweet Hereafter" picked up eight trophies Sunday night at the Genie Awards, Canada's answer to the Oscars.
"The Sweet Hereafter" picked up eight trophies Sunday night at the Genie Awards, Canada's answer to the Oscars.
Paramount Domestic TV has firmed up the future of "Hard Copy," renewing the syndie newsmag strip through 1999 in 19 of the top 20 TV markets.
Warner Bros.' "L.A. Confidential" was a hero at home, winning four top honors from the Los Angeles Film Critics Assn., including best picture.
NBC Sports has inked a four-year extension of its U.S. Open golf pact that will keep the prestige links tournament on the Peacock web through 2003.
Lee David Zlotoff, the creator of TV's "MacGyver" and the writer-director of the indie pic "The Spitfire Grill," is set to take the helm of "Zero Hour" for 20th Century Fox.
Toshiba Corp. and Matsushita Electric Industrial Co. said Monday they would invest 595.5 million yen ($4.56 million) to launch a digital videodisc rental service with Japan's largest video rental…
Liberty Media Group CEO Robert Bennett said Liberty's investments in Discovery Communications and Encore Media were "extraordinarily unlikely" to be among assets transferred to Barry Diller's HSN…
Fox is taking sports electric, hoping that the success of its four-year-old sports television broadcast programming will rub off on a new sports-themed line of video games.
The son of Bavarian media mogul Leo Kirch has founded Thomas Kirch Communication GmbH & Co. KG Medien Holding, Munich-based news service New Business reported over the weekend.
In one of the strongest trading weekends of the year at overseas wickets, James Bond conquered the U.K., "Anastasia" had bewitching foreign preems in two Latino territories, "Hercules" kept pulling…
The growing Universal Music Latin America has further beefed up its ranks by inking industry vet Carol Wright to the newly created post of veep of marketing.


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Time Warner Cable, Viacom Reach Agreement
Time Warner Cable and Viacom jointly announced January 1 that they have reached an agreement in principle to renew carriage for Viacom's MTV Networks. The companies expect to finalize the details of the agreement over the next several days.
Glenn Britt, President and CEO of Time Warner Cable said in a statement, "We are pleased that our customers will continue to be able to watch the programming they enjoy on MTV Networks. We are sorry they had to endure a day of public disagreement as we worked through this negotiation."


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The Beginning of the Blog
I am starting this blog for a Mission trip that I am taking to Thailand in February 2016. Our group is a medical, dental, chiro trip to care for the people of northern Thailand. Centered in Chiang Rai the trip will be 10 days in support of the ...
Caleb and the 8 Mile City
Up at 6 today. Pulled over by 8:30 for being in the wrong lane. The cop was also a DOT officer and very chatty...he told us all about the foreclosed house he just bought...he also said Jp does a nice job with his log books. Detroit is boarded up, burned out, and run down. Huge buildings with no windows left...every other house abandoned.... Jp and Caleb delivered in a rather wealthy subdivision outside of the city. I laid on a furniture pad in the shade of the truck and read while Jp ...
Save Ukrainian Bird Savchenko
Russia still does not allow the Ukrainian consul to see Savchenko, a Ukrainian aviator, who was illegally taken to the Russian Federation and who is kept in jail in Voronezh. The Consul General of Ukraine in Moscow Gennady Breskalenko, who are trying to get an access to the detainee for several days now, told this on Saturday to UkrInform. "As of today, I have not met with Nadia Savchenko ...
Feral Foods CSA
... is a small garden at the house (largely for Britt, Pat, and Marla) and a larger patch of land, about 2 acres, on which more food is grown. CSA members pay for a bundle of food which is collected each week (either tuesday or thursday, in this case), or, for a reduced price, CSA members work 2 hours a week weeding, harvesting, and/or cleaning produce for everybody else. It truly does show a sense of community, especially when half a dozen people show up at the same time ...
... This is a classic canyon-country hike with steep canyon walls rising from the creek. At some points, the canyon is no more than 20 feet wide, with walls rising up more than 200 feet. You can also extend the hike many more miles up the canyon for an overnight backpacking trip. The trail head for the West Fork of Oak Creek hike is 9 1/2 miles up Oak Creek Canyon from Sedona at the ...


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Ofir Nachmani: Cloud Developers are from Mars
Ofir Nachmani presents Cloud Developers are from Mars:
The three layers of cloud computing, IaaS, PaaS and SaaS, occupy the headlines, with significant capabilities undergoing continuous improvement to host services in the cloud. This growing market is slowly changing so that offered services will become generic. The current evolving struggle is the deployment and management of SaaS...
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well that's a waste of good food I hope they didn't eat it after doing that must give the food a spicey taste eeeeeeeeeeeeeeeeeeeee lol
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All Comments (46)
well that's a waste of good food I hope they didn't eat it after doing that must give the food a spicey taste eeeeeeeeeeeeeeeeeeeee lol
Vulture that thing at the last min is a massive big dildo
That was the strangest thing I have ever seen I my life... Wow.
That was pretty interesting nasty but hot but i know i will never do that!!!!!
Un brindisi alle pannocchie in culo !!! Porcamadonna
Was hat die für ein Arschloch. Die Gurke würde ich nie in meines kriegen
Now, after jerking off, i'm hungry.... I don't know why ;-)
Tasty :)
That was fucking wild!
Omfg that was great but I will never do that ever.I don't think my tight pussy could fit that massive thing at the end.
What a fabulous vulva !!!
I love to do that kind of crazy things with my husband...love crazy sex
Ich wuerde die Gurke noch warm und schleimig lutschen
Gotta find me a women like that
what does cum taste lke im a virgin and never masterbated just rubbed once or twice but only till it starts to thump i cant take it
Uff I'm cumming so such Fuck me fuck me fuck me
OMG!!! I want a girl who is crazy and hot like this girl!!!
dont fancy eating at her house
Corn is so fucking hot to fuck - I love feeling the ridges as it goes in and out - so fat - makes me so wet and I finger my clit and make me come while the cob is deep inside. Wish I could share with another girl - would love to lick a clit and taste it, make drip all over my face.
I do not like the way he talk to her. Sure she was doing interesting things with a cucumber when he walked in... but that does not make her a slut. how rude.
now the meat is disgusting sry but ewww
this is the way sex should be
Die Zucchini find ich auch gut
the food ruins it
ok i like that
Man ist das pervers, so ein ausgefranstes Arschloch hab ich ja noch nie gesehen. Arschfisting vom feinsten... Aber die Hänchenkeule igittigitt...
the girls nam is audrey hollander
Audrey, best fucking girl ever.
na der ihre Muschi ist aber auch ein klassischer Eintopf. Fehlt nur das sie sich eine Wassermelone einpflanzen lässt
Different foods are Girl's BF
after the size of that veg, she must be disapointed with that cock
Mir is n Schwanz lieber
Now I'm hungry...
Putin , elle est chaudecette meuf
Hey, at least she's staying healthy and getting two servings of meat and vegetables
alter bei mir geht dass nciht:///
übertreiben kann man es auch..
last minute! What the hell is that thing??
Das ist ja ein Hammer!!!! Super Geil!!!
WOW what a super lady !!!!


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Tad DeHaven is a budget analyst at the Cato Institute. Previously he was a deputy director of the Indiana Office of Management and Budget. DeHaven also worked as a budget policy advisor to Senators Jeff Sessions (R-AL) and Tom Coburn (R-OK).
The Greatest Assassination Plot in History Comes to Life in New Book, "Operation Long Jump" | Leah Barkoukis
House Complicates Iran Deal by Passing Bill to Make Iran Compensate Terror Victims | Cortney O'Brien
Pew poll: Trump leads GOP field by nine as Jeb Bush crashes into sixth place with … four percent | Allahpundit


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12/22/2009 | ARRL Seeks Emergency Preparedness and Response Manager
The ARRL currently has an opening for an Emergency Preparedness and Response Manager to be based at ARRL HQ. The successful applicant will represent the League with governmental and non-governmental emergency and disaster response organizations and partne
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Feature12/02/2009 | ARRL In Action: What Have We Been Up to Lately?
This feature -- including convenient Web links to useful information -- is a concise monthly update of some of the things ARRL is doing on behalf of its members. This installment covers the month of November.
Learn More
11/24/2009 | Incumbent Section Managers Win New Term of Office
Three incumbent Section Managers were re-elected in races that concluded on Tuesday, November 24. In Alabama, Jay Isbell, KA4KUN, received 513 votes; challenger Les Rayburn, N1LF, received 167 votes. Isbell, of Bessemer, will begin his second term as Sect
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10/28/2009 | Former Hudson Division Director Paul Vydareny, WB2VUK (SK)
Paul Vydareny, WB2VUK, of Tarrytown, New York, passed away Thursday, October 22, from a brain aneurism. He was 65. An ARRL Life Member and member of the ARRL's A-1 Operator Club, Vydareny served as Director of the ARRL's
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10/06/2009 | 2009 Section Managers' Workshop: A Learning Experience
The Annual Section Manager (SM) Workshop -- held September 25-27 -- afforded 12 newly elected SMs (including three that began their first terms of office on October 1) to visit ARRL Headquarters. During the extended weekend gathering, participants not onl
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09/23/2009 | Newly Elected Section Managers Converge on Newington
This weekend, 12 Section Managers who are new -- or returning after a long absence -- to their post are in Newington for an orientation. The primary purposes of the Workshop sessions are to share ideas and to provide basic administrative, management, lead
Learn More
Feature09/01/2009 | ARRL In Action: What Have We Been Up to Lately?
This feature -- including convenient Web links to useful information -- is a concise monthly update of some of the things ARRL is doing on behalf of its members. This installment covers the month of August.
Learn More
08/24/2009 | ARRL Section Manager Election Results
Of the nine Sections holding Section Manager elections this month, three will be getting new Section Managers: Los Angeles, South Texas and Georgia. The Sacramento Valley, Eastern Washington, Colorado, Western Washington, West Virginia and San Francisco S
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By Samantha Leal | 10/01/2014 - 11:02
Talk about a hot date!
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By Cristina Arreola | 09/26/2014 - 12:00
Okay, Sofia Vergara, we get it! You have a really, really sexy new boyfriend, Joe Manganiello.
Read more
By Priscilla Rodriguez | 09/25/2014 - 13:07
What broke the ice between you and your significant other?
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By Cristina Arreola | 09/23/2014 - 12:16
When Jimmy Fallon interviewed Sofia Vergara on Monday night's episode
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By Priscilla Rodriguez | 09/23/2014 - 10:25
The secret to snagging a man? Sofia Vergara says you gotta smell good!
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By Priscilla Rodriguez | 09/20/2014 - 11:00
Happy birthday, Sophia Loren! The iconic Italian actress turns 80 today. Throughout her career, Loren has managed to inspire millions of women across the globe, including these lovely Latinas:
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By Andrew Villagomez | 09/17/2014 - 15:50
Modern Family is returing for its sixth season on September 24, and we can't wait to catch up with the Pritchetts, Tucker-Prtichetts and Dunphys.
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By Priscilla Rodriguez | 09/09/2014 - 17:40
Just like us, celebrities sure do love their sports! From Sofia Vergara walking the field before a football game to Eva Longoria supporting her pal at the US Open, check out these 13 Latinos who love a good game/match/tournament:
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By Cristina Arreola | 09/09/2014 - 11:41
Sofia Vergara's ex-fiancé, Nick Loeb, has finally come clean about his true feelings regarding
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By Cristina Arreola | 09/08/2014 - 15:01
Sofia Vergara stood up to cancer this past weekend — with a few good friends by her side.
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Latest Headlines
Latest Headlines
Microsoft to introduce flagship Lumia phone running Windows 10
Microsoft is betting that a unified platform for PCs, laptops, smartphones and tablets will make its smartphones more attractive to the enterprise. As part of that effort, Microsoft announced Monday that it will be introducing a flagship Lumia smartphone running Windows 10 later this year.
More mobile malware will accompany BYOD devices into enterprises this year
This year will be an "inflection point" where BYOD devices and malware targeting those devices proliferate in the enterprise, judges Andy Daudelin, vice president of security services for AT&T's Mobile Business Solutions team.
News Scan: CIOs warned against falling into complacency mode; AT&T announced mobile data rollover plan; More.
The top news stories for Jan. 8, 2015.
Companies fret over California BYOD court ruling
The ruling last year by a California appeals court about BYOD expense reimbursement has caused consternation among enterprises throughout the country. Any enterprise that allows BYOD should prepare for the implications of the California court ruling, as well as the expansion of the reimbursement requirement to data and to other regions of the country.
AT&T moves to broaden BYOD offerings
To address the growing BYOD market, AT&T is teaming with MobileIron, AirWatch by VMware and Good Technology to offer a new BYOD option that enables firms to add AT&T data, voice and messaging service to their enterprise mobility management platform.
AT&T moves closer to making a browser work like a phone
A beta program for a new API has just been opened to AT&T's Premium tier enterprise customers, enabling developers to claim new telephone numbers for their Web servers.
Cricket thwarts encryption, faces mounting criticism from privacy advocates
AT&T prepaid mobile service subsidiary Cricket is facing increasing criticism for preventing email sent by some of its customers from being encrypted, reports Ars Technica.
It's war: AT&T CEO to suspend broadband buildout, claims FCC's Wheeler for his side
If Randall Stephenson's comments are to be taken at face value, the FCC chairman actively worked to avoid a Title II scenario. But those negotiations fell apart after the mid-terms.
Telcos mount opposition to Obama's 'Third Way' for net neutrality
The President could not have been unaware his decision to back Title II reclassification of the Internet as a common carrier would only come after a long, hard fight.
News Scan: Judge orders documents released in GT bankruptcy; Verizon, AT&T work on VoLTE interoperability; more
Check out the hottest mobile IT news for Wednesday, Nov. 5, including a federal judge's orders to publicly release documents related to the bankruptcy of Apple's sapphire supplier GT, Verizon and AT&T's work for VoLTE interoperability by next year, Google and LG's long-term patent cross-licensing agreement, retailers targeting mobile promotions to capitalize on unique features of smartphones and the 49ers use Aruba Networks stadium beacon network and location-based app.


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A continuation of our study of Plutarch's (46 - 120 AD), Lives of the Noble Greeks and Romans(commonly called Parallel Lives or Plutarch's Lives).
This work is a series of biographies of famous Greeks and Romans, arranged in pairs to illuminate their common moral virtues and vices. As is explained in the opening paragraph of his Life of Alexander, Plutarch was not concerned with history so much as the influence of character, good or bad, on the lives and destinies of men.
This month we will examine the Life of Romulus. As we studied Theseus last month, here is Plutarch's Comparison of Romulus with Theseus.
I started the group because there wasn't any other type of group like this. I've met some great folks in the group who have become close friends and have also met some amazing business owners.
— Bill, started New York City Gay Craft Beer Lovers
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text: Melrose Place Clip Matt Richenthal at October 27, 2013 8:58 am. Comments These are clips from the series premiere of Melrose Place on The CW. In the first, Laura Leighton returns as Sydney; in the second, hey, that's Ashlee Simpson! Share Tweet Email Edit Delete Follow Melrose Place Show: Melrose Place Related Videos: Melrose Place Videos Uploaded by: Matt Richenthal Uploaded: October 27, 2013


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October 2nd, 2015
The 10th year of the Follow the Light Foundation awards is now open for entries. If you or if you know aspiring photographers, we are awarding a $5,000 grant and adding to an impressive list of accomplished photographers who got their start from the this foundation in memory of the late Larry "Flame" Moore. CUTOFF FOR SUBMISSIONS: JUNE 15.
Learn more and get submission instructions at Followthelightfoundation.org
Thank you for visiting the Swell blog. Visit us on www.facebook.com/swell for more exclusive fan offers, giveaways and more.
Tired of the vicious cycle of spinach wraps and Sepia filters on Instagram? Break the mold and curate a gallery of double-tap worthy adventure shots—it's not as hard as it sounds. Most of the time, creating a gorgeous Instagram gallery isn't about where you are, it's about how you shoot it (because any outdoor excursion, big or small, is pretty dang beautiful). And keep in mind, Instagram isn't just about racking up followers—it can be a great way to revisit your favorite moments and connect with old friends easily. Here, eight tips for taking better outdoor Instagram shots you'll definitely "like" for a long time to come—even if you aren't a professional photographer.
Figure out what your goal is with your gallery—is it to tell a story? Chronicle your life? Score a sponsorship? Let that dictate what your photos look like.
Tip #1: Rethink Your Instagram
My friend Kat Carney, who happens to have one of the most beautiful Instagram galleries I've seen yet, once told me to think about my Instagram as if I were curating a small, square art gallery—advice that's totally changed the look of my own account! Instead of snapping everything and anything and posting it, try being pickier with what you share with the world. Just like you'd edit a real-life photography gallery to fit a theme, your Instagram should reflect a specific mood or story. Choose yours and post accordingly! (more…)
New to the SWELL Surf Shop, Ollo Lenses lets your phone capture museum worthy shots. Friend of SWELL Chris Burkard swears by the clip-on lenses, so maybe you'll also get half a million instagram followers with Ollo clip-on Lenses
1. Focus on what's important with the Fisheye lens.
Before shooting with a fisheye lens, determine the most important elements in your composition (placement or arrangement of visual elements). The subject you focus on will be sharp and clear but peripheral elements that are away from the center of the picture will have a characteristic fisheye stretch. Background elements are generally secondary when using this lens, so your foreground subjects must be able to carry the composition and provide power to the image.
2. Keep the foreground free. (more…)
Winner of Sanuk's #TotallyBeachin Instagram contest, Orange County grom won himself a trip to shoot photos at the Pipemasters. We caught up with the little legend to see what he learned under the guise of lensman Brian Bielmann in the impact zones at Pipe & Wedge. Follow @LukeForgay
"I like to shoot in the water as much as I can, the angles that you are able to capture in the water are second to none. Whether shooting fisheye or a longer lens in the water, angles can make or break a great photo just like with anything in photography. My favorite to shoot in water is a fisheye lens and with the surf photography market flooded with fisheye photos there are ways to differentiate your style." (more…)


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MyEssayWriting.com - Essay Writing Services.
Anna at the Supermarket - Oxford Storyland Readers - Level 2
BBC Knowledge - October 2015
Chicken Soup for the Preteen Soul 2
At last …the long-awaited follow-up to the 1.2 million-copy bestseller! Filled with relevant, inspiring and fun stories written mostly by kids, this new volume features a unique, innovative chapter, "Rockin' Our World", that highlights real kids achieving real dreams, helping less fortunate people, starting your own business, and utilizing your unique abilities. For a young generation repeatedly exposed to a hostile world, this must-read book promotes positive thinking, giving to others, unity and tolerance.
Reuploaded Thanks to emkis


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Wanderlei Silva and Rafael Cordeiro visit Mark Munoz gym Reign in Lake Forrest California, to train with a team of top MMA fighters, including Fabricio Werdum, who is preparing to fight Fedor Emelian watch video >>
Mark Munoz speaks to Fight Hub TV about his UFC 123 matchup with Aaron Simpson. Munoz says he did not want to fight his friend at first but it is his job to do so. He also will not be worried landing watch video >>
Todd Duffee Talks to Alistair Overeem Backstage After Their Dynamite!! 2010 Bout watch video >>
A re-focused Andrei Arlovski talks training for Sergei Kharitonov and his career at the Heavyweight Grand Prix Photo shoot. http://strikeforce.com watch video >>
Mark Munoz talks to Megan Olivi at the Throwdown Training Center in Las Vegas about his life, career, upcoming fight with Demian Maia and much more. watch video >>
Paual talks to Demian about his preparation for his upcoming fight versus Mark Munoz at UFC 131: Dos Santos vs Carwin. Watch the event Live on Pay-Per-View or www.UFC.tv this Sat Jun 11th at 6/9PM P watch video >>
UFC middleweight Mark Munoz spoke to Fight Hub TV sports correspondent Marcos Villegas about his UFC 131 matchup with Damian Maia. watch video >>
MMA H.E.A.T.'s Karyn Bryant talks with Cezar "Mutante" Ferreira, a young Brazilian fighter who is Vitor Belfort's protege. Cezar talks about his career as a Capoiera fighter, his training in the US watch video >>
Krzysztof Soszynski talks to GnP-TV about his team-mate Mark Munoz and his upcoming bout against Igor Pokrajac. watch video >>
Mark Munoz talks to us about just how many Mark Munoz's there are, why its not funny getting hit in the back of the head and how he and Chris Leben will collide like "rams going at it" @fightersonly watch video >>


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