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Search Results: 'Nicholson'
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This is a compilation of quotes from Mr. Nicholson's Challenge I class of 2014
Meredith Nicholson, Collection
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Meredith Nicholson (December 9, 1866 – December 22, 1947) was a best-selling author from Indiana, United States, a politician, and a diplomat. He wrote Short Flights in 1891, and continued to... More > publish extensively, both poetry and prose until 1928. During the first quarter of the 20th century, Nicholson, along with Booth Tarkington, George Ade, and James Whitcomb Riley helped to create a Golden Age of literature in Indiana. Three of his books from that era were national bestsellers: The House of a Thousand Candles (#4 in 1906), The Port of Missing Men (#3 in 1907) and A Hoosier Chronicle (#5 in 1912) In this ebook: The Main Chance The Siege of the Seven Suitors Rosalind at Red Gate Otherwise Phyllis The Madness of May Blacksheep! Blacksheep! Lady Larkspur A Hoosier Chronicle A Reversible Santa Claus The Port of Missing Men The House of a Thousand Candles< Less
A Hoosier Chronicle by Meredith Nicholson
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A Hoosier Chronicle by Meredith Nicholson. Considered by many to be his best work, the novel examines Indiana society and politics at the turn of the century. The book begins: Sylvia was reading in... More > her grandfather's library when the bell tinkled. Professor Kelton had few callers, and as there was never any certainty that the maid-of-all-work would trouble herself to answer, Sylvia put down her book and went to the door. Very likely it was a student or a member of the faculty, and as her grandfather was not at home Sylvia was quite sure that the interruption would be the briefest.< Less
Jack Nicholson - Biography Series
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Ever wondered how Jack Nicholson rose to stardom? Jack Nicholson is without a doubt one of the most popular and gifted American actors of the 20th century. The characteristic features of Jack... More > include the dark tinted sun glasses, without which he is rarely seen outdoors, and the somewhat maniacal grin which has a great hand in shaping up his on-screen persona as a psychotic, neurotic and enigmatic character. The real Jack Nicholson is not far from what he portrays on his films: he smokes heavily, drinks heavily, has casual dates with multiple woman and even smokes on cannabis. Nicholson first came into the spotlight with the movie Easy Rider in 1969 and also with the subsequent success in Five Easy Pieces in 1970. His ability to assume a range of unconventional characters is greatly appreciated among his fans. For more interesting facts you must read his biography. Grab your biography book now!< Less
Andrew Nicholson's Vision Statement Jan 2007
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Andrew Nicholson's Vision Statement January 2007. A powerful statement of intention that reinforced and accelerated his purpose and success in life.
Andrew Nicholson's Vision Statement Jan 2008
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Andrew Nicholson's Vision Statement January 2008. A powerful statement of intention that has reinforced and accelerated his purpose and success in life this year - back in the UK with Diane. This... More > revision illustrates the fluidity of the 'how' together with the consistency of the Authentic Purpose - the 'Why'.< Less
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Nicholson-Jodoin Calendar 2009
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A Memoir
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A memoir of Australian writer and film-maker Michael Nicholson.
The Madness of Day
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The Madness of Day by Meredith Nicholson and Illustrated by Frederic Dorr Steele, first published in 1917. "May to Mr. Nicholson is neither a person nor a month. It is a state of mind and an... More > intoxication of spirit. The little tale is a gay and joyous fantasy that plays with the imagination like the wind through new-leafed trees."?—New York Sun. "No one who wishes to be charmed out of himself or herself for an hour or so should neglect to read the story."?—Philadelphia Ledger. "Meredith Nicholson has written nothing more charmingly fanciful or more filled with the spirit of the springtime than 'The Madness of May.'"—St. Louis Republic. Meredith Nicholson (1866–1947) was a best-selling author from Indiana, United States, a politician, and a diplomat.< Less
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Meetings & Events
Versatile meeting rooms for impressive Spa eventsIn an ideal location adjacent to Les Thermes de Spa, the Radisson Blu Palace Hotel is an innovative venue for a variety of occasions. Spanning over 300 square meters each, the accommodation's two meeting rooms equip Spa event planners with everything needed for a successful gathering. Adaptable and dynamic, the meeting space can be divided into as many as eight separate rooms to best suit your event.
Meeting Room Features
Our eight stylish conference rooms can be tailored for your individual occasion, whether you're hosting an intimate board meeting or a banquet for 150 guests. If you're planning a reception or a barbeque, you can take advantage of our inviting outdoor terrace.
Capacity Chart
Key features:
- Business center
- Customized menus from the hotel's experienced chef
- Connections for audiovisual equipment and telephone lines
- Dedicated Meetings & Events coordinator
- Farewell refreshments
- Free high-speed, wireless Internet for meeting delegates
- Individual climate control
- Presentation technology including podium, flip chart, LCD projector, wireless microphone and white board
- Standard office equipment, such as pens, markers, note pads and correctors
Capacity Chart
Meeting Packages & Events
Event planning is simplified with our three special meeting packages: Fresh, Host and Easy. Each of these selections includes coffee breaks, room rentals, lunch and other items based on your needs and desires. Our experienced Meetings & Events staff welcomes the opportunity to help you organize team-building activities, product launches and more.
Meeting and conference suggestions:
- Outdoor themed dinners in the Abbaye de Stavelot, the Casino and L'Eau Rouge (located on the F1 racetrack of the Spa - Francorchamps) or in the Domaine of Berinzenne in the middle of the natural beauty of Ardennes
- Team-building activities, including four-wheeling, mountain biking, parachute jumping, golfing, kayaking and caving
- Product launches, car launches and test drives on the F1 racetrack of Spa-Francorchamps


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**** the dees are frustrating, its been the same problems for years. their defensive pressure is so ****ing trash and it gets exploited cause they turn the ball over so much as their use of the ball is ****ing horrible aswell. the amount of times they just drop regulation marks is ****ing embarrassing
no hustle either skip
Brad McNamara @bbuzzmc
Will say this once and then nothing else. Defamation laws quite clear in Aus.be careful.
it's not like that
melbourne have scored 83 points and still nearly lost by 100 wtf is that
Healy called Curtley Hampton Curtley Ambrose.
Who do you guys reckon will win next week's test match?
WWCC - Loyaulte Mi Lie
"Hope is the fuel of progress and fear is the prison in which you put yourself" - Tony Benn
#408. Sixty three not out forever.
Just realised that I had Swans Under 39.5 as part of a multi. Who scored the last point in the game?
NRL Tipping Champion 2014
5 of the worst kicks for goal ever (R3 Essendon v Fremantle) - YouTube
God dammit footy.
I am a brave man, I am a coward. I am the tiger, I am the flower.
I am honest, and I'm a liar. I am vital, I am tired.
I am a free man, I am caught. From where I am, I see the top.
I am most importantly never concerned with what I am not.
Forever, more than just surviving.
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Bed, breakfast and beyond awaits you, Adirondack style. Each room has a fireplace, private bath with claw foot tubs/shower, comfortable beds, A/C and free wireless. Our guest receive complimentary beverages and can help themselves to the endless cookie jar.
The guest living room features a fieldstone fireplace, cable TV and comfortable Arts and Crafts style decore. Our breakfast has been referred to as "a no lunch" breakfast by some of our clients. Our eggs come straight from our girls (chickens). Rock and relax on our wrap around porch with screened in area or sit in our Adirondack chairs with tranquil views of the lake and Pharoah Mountain. We offer country gardens, a picinic table, a gas grill and outdoor hot tub.
We are 1/2 mi from the center of the village, stunning town beach , boat launch ramp which are free to the public. There is hiking galore in the region, tennis, golf, the Seagle Music Colony with superb summer performances and many community events. We are about 1/2 hr from Lake George and 45 min to Lake Placid which makes for a nice day trip, leaving the crowds behind.
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BedandBreakfast.com gift cards are the perfect gift for anyone who needs a relaxing getaway.
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on July 25, 2015
My wife and I stayed on a Friday night in July. We were met by the owner and given all the information we needed to enjoy our stay. The husband and wife team were friendly, happy to talk, and help with any of our needs or questions. The restaurant the owner suggested us to go to for dinner was excellent. Our room was clean and had a nice private bathroom. We would stay at the Tumble Inn again if we needed a room in that area of the Adirondacks.
Innkeeper ResponseThank you for writing a review. We are pleased when our clients are happy. We are glad you enjoyed your stay. Perhaps you will come again.
on July 03, 2015
Nancy and Larry are welcoming and very accommodating. They made us feel at home immediately. They have a lovely cozy house with a beautiful wrap around porch you just have to enjoy. The minute I stepped into the home I could smell Nancy's famous cooking. We stayed in the beautiful Spring Room. Everything was tidy and clean. Her bed sheets were amazingly comfy and so was the bed. They thought of everything to make the guest experience the best. Nancy is an impressive creative cook who always tries to outdo herself for the next breakfast meal. Her Apple Stuffed French Toast were so good I took the recipe home with me. This was my first B&B experience and I found it better than a hotel. Nancy and Larry run a first class B&B. I highly recommend Tumble Inn. Paula & Armand Chartier
Innkeeper ResponseThank you Paula. We appreciate hearing feedback that we are doing something right. We enjoyed having you and we greatly appreciate the note you sent to my grand nieces that help me with the cleaning. They were thrilled! Hope to see you again.
on June 01, 2015
The room was beautiful! Our host went out of her way to make us welcome and comfortable. Breakfast was like a 5 star hotel would serve. We will stay here again.
Innkeeper ResponseAnd we hope to see you again soon.
on April 23, 2015
Very nice accommodations, very friendly owners.
Innkeeper ResponseWe are glad you were satisfied and only hope that we can continue to improve. Thanks you for the review.
on April 23, 2015
Quick visit, a long road...excellent find! Good company and great breakfast..
Innkeeper ResponseThanks for taking the time to leave a review. We are glad that you found our inn pleasing.
on April 20, 2015
Beautiful home, delicious breakfast, lovely gardens, comfortable accommodations, close to Seagle colony
Innkeeper ResponseThanks. We are preparing for the 100th Anniversary of the Seagle Colony this summer and hope to host many visiting guests for the special events. Do come again.
on April 16, 2015
We enjoyed our stay at the Tumble Inn. Very quaint old home. Nancy is an excellent hostess and cook!!
Innkeeper ResponseThanks for the compliments! We were recently voted Best B and B by the readers of Adirondack Life magazine and we are very proud to have satisfied customers.
on April 16, 2015
Breakfast was extraordinary and innkeepers were helpful and friendly
Innkeeper ResponseThank you for the review. We will continue to make changes that make our guests comfortable.
Really enjoyed our overnight stay, long walk and serene time spent on the lake. Beautiful surroundings. Inn incomparable comfort. Host, Nancy, makes this a beautiful respite for one to return to.
Innkeeper ResponseI think your review shows the essence of our inn. It is not ornate, but a comfortable farmhouse with character. And, my reason for being here, the natural beauty that surrounds me each day. Glad you enjoyed my part of the world!
on April 08, 2015
Nancy and Larry were great hosts, place was clean, quaint and in a beautiful setting. Room was cozy and comfortable. Breakfasts were outstanding
Innkeeper ResponseWe thank you for choosing our in and for posting a review. We hope you will come again.
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Roses and the hues of an Adirondack sunset invite you to relax. Offers a partial lake view and a king sized bed (or two twins). Private bath with a claw foot tub and modernized shower. Electric fireplace offers summer ambiance and winter warmth. A third person may be added for an additional $15.
Our most requested room. It has high ceilings and offers filtered lake views with a king sized bed. The decor is
marked by spring violets and mirrors. A comfortable chair and writing desk add to its appeal. There is an electric fireplace for summer ambiance and added winter warmth. The private bath features basket weave wainscoting and a claw foot tub with modernized shower.
A room with a feeling of being on the slopes of yesterday. Decorated with an Olympic flair. Features a queen sized bed, with a private bath housing a claw foot tub and modernized shower. An electric fireplace adds summer ambiance and added warmth in the winter.
The colors of fall and changing leaves make this a vibrant room. The room has a king sized bed (or two twins)with a private modernized full bathroom with double sinks and granite countertop. An additional room with 2 twins can be added for an additional $75 making this an excellent choice for families or groups of four.
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Albany, NY (ALB-Albany County) 60 miles
From the South
Take I-87 (Adirondack Northway) to exit 27. Turn left onto State Route 9 heading north for about 4 miles. We are on the left side of the road between Charley Hill Rd. and Hoffman Rd.
From the North
Take I-87 (Adirondack Northway) to exit 28. Turn right onto State Route 9 heading south. You will pass through the town of Schroon. We are approximately a half mile south of town on the right side of the road between Hoffman Rd. and Charley Hill Rd.
Amusement Park,
Antique Shopping,
Art Galleries,
Concert venue,
Gourmet/Fine Dining,
Historical interests,
Live Music,
Movie Theater,
National/State parks,
Nature area,
Caves, Rafting,
Outlet Stores,
Performing Theater,
Picnic area,
Religious Services,
Ski area
Private Home Bed and Breakfast, Bed and Breakfast, Country Inn
BBQ grill, Board games, CD player, Computer, Copier, DVD Player, Desk, Fax Machine, Hot tub/whirlpool tub, Library, Satellite/Cable TV, TV, Telephone, VCR, Videos, Washer/dryer ,
endless cookie jar, complimentary soft drink
Air conditioning, Alarm clock, Amenities Basket, Bath robes, Ceiling fans, Fireplace, Hairdryer, Internet Service, Ironing Board, Radio, Wi-Fi, Wired internet
Bicycle riding trails, Billiards, Bird watching, Boating, Canoeing, Cross-country ski trails, Downhill Skiing, Fishing, Golfing, Hiking trails, Horseback riding, Horseshoes, Hunting, Kayaking, Mountain Bike trails, Sailing, Shopping, Sight-seeing, Ski rentals, Snow shoe trails, Swimming, Table Tennis, Tennis courts, Video Arcade, Water sports
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Nancy and Larry
English, Italian
I am a retired helath/PE teacher. I have loved the Adirondacks and all it has to offer since a young child. Each season brings a unique offering: skiing, x country skiing, snowshoeing, hiking, swimming, kayaking, boating, waterskiing or tubing, white water rafting, golfing, volleyball (I used to coach), tennis, pickleball... I love it all. On the quiet side, I do enjoy sewing, reading and crafting, but it's hard to get me to sit still.
Low fat , Vegetarian
$98 per night
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Innkeepers: Nancy and Larry
Guests: 2
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Federal CIO Steven VanRoekel has quickened the pace on the governments data center consolidation efforts, and simultaneously broadened the field.
Agencies are now to look at almost any facility that includes servers, even wiring closets of as little as 100 square feet, as a candidate for consolidation and closure, he said in a conference call. Previously, the assessment of data centers focused on facilities of 500 square feet and larger.
VanRoekel said the government now hopes to shutter 472 facilities by year's end and 962 by 2015. Previously, the Office of Management Budget set goals of shuttering 373 data centers by the end of 2011 and 800 by 2015, with a goal of saving $3 billion annually. Agencies published their latest plans for consolidation on CIO.gov on Oct. 7.
Related coverage:
Do consolidation and cloud always kill jobs?
Kundra heralds 'golden source' of data center savings coming soon
The government has closed 81 data centers to date and now envisions saving $630 million in the near term with the current consolidation plans and $5 billion savings in the years beyond 2015, VanRoekel said.
Agencies will post data center consolidation plans online on Oct. 7. A more comprehensive agency-to-agency breakdown will be available at the end of the year, VanRoekel said.
OMB has also launched a new data center cost model "to drive an apples-to-apples comparison on the way agencies budget and drive behavior on closing data centers," VanRoekel said. The cost model will give OMB and CIOs across agencies the tools to compare with other agencies whether they are doing the right things to close data centers.
When VanRoekel became federal CIO, he saw a lot of inconsistencies in both the way agencies were managing their closure planning and data center optimization. Working with the Data Center Consolidation Initiative Task Force, which has 24 agency members, a tool was created that can more effectively help agencies manage and budget for data center optimization and closures, VanRoekel said.
About the Author
Rutrell Yasin is is a freelance technology writer for GCN.
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yeah... we consolidated... moved our equipment from our leased space (which we still have to pay for) to another agency that charges us more than what we paid at the previous place... consolidation = yes; savings = NO
Other than maybe a little electricity, how does closing a data center in a govt-owned building save the taxpayers a dime? (Note I said taxpayers, not agency budgets. The money paid from one agency to another for 'rent' never really existed.) What is the payback period for these forklift moves or virtualization projects, especially for legacy systems with less than 5 years expected remaining lifespan? I'm strongly in favor of consolidated data centers, but it has to be done sensibly. From what I have seen of the current project so far (from down on the bottom), this is a stunt so they can say they 'did something', and the costs are irrelevant.
I am amazed at how closing data centers will make us more efficient. It seems akin to closing your shooting your business foot off. How can agencies survive without data centers? CIO UR expecting too much from PCs. Maybe if you had worked at a federal data center you would see that. Consoladate yes, close no. Stupid idea.
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Hotel Mira Spiaggia
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Trapani and Erice
... but that was way too ambitious. Instead we drove back to our apartment in Palermo to relax a bit before heading out later to a streetfest type event that was going on near us. I don't know what all they were cooking on the grills, but one vendor said if you come to Palermo this is what you eat! uh... thanks but no thanks`. I liked the idea of trying street food the locals eat, but in this case I had no ...
The storage day
Early in the morning my english crew had to leave to get back to their reality and at 7.30 a.m. It was time to say good bye. The whole day I was busy with organizing our next large day and night trips. I made friend with the very kind owner of a supermarket and he helped me to get along with all the the stuff to drink and to eat which are needed in the next couple of days on sea. The list was huge: 60 liters of mineral water 80 tins of coca cola 40 tins of beer 30 bottles of ...
Cefalu to Terrasini
We had a fun day yesterday exploring Cefalu. It's a very charming town, with lots of tiny side streets all seeming to go towards the church. Aggie yet again made fresh pasta and had many admiring looks from other crews as we tucked into our delicious feast. We left Cefalu at 7am today as the wind could not make its mind up which direction it was going to blow ! We were graced again with dolphins jumping out of the water nearby. It's surprising how ...
Blue sea, hot sun and 'leetle bit of english'
... we didn't have a lot of money they were very worried about us. When we arrived they give us 20 euro and made sure we would buy water and food. We were so thankful but forgot to ask their names. So with water and food in our hands we were looking for a place to sleep. After a while we met a Romanian family, who would offer us a place to sleep. We could stay in there house. It was a bit weird at first but we had ...
South in the sun
... issues etc. In the last couple of days we have noted three abandoned vessels of varying sizes that have been notified as a hazard to shipping and also a message about military activity in the sea north of Libya. The advice we have received is to stay well clear of any migrant boats we might come across and the reality is that they rarely would ever get this far north without being picked up, but the traffickers ...


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By calculating the amount of food lost because of dropping sea levels, and combining this with estimates of the food needed to keep a whale alive, the two researchers calculated the impact of global cooling on gray whale populations and the populations that would have had to exist in order for the whales to survive.
They concluded that populations would have had to have alternative feeding modes sufficient to support a population of around 70,000 during warm periods so that population drops during glacial periods wouldn't be below 5,000-10,000 whales. Much lower numbers would have produced a genetic bottleneck obvious in the DNA of the whales, and such a signature has not yet been seen.
"We don't yet have the ability to look deep enough into the whale genome to see this type of bottleneck," Pyenson added, though genetic analysis that has been done shows no evidence of a bottleneck much shallower in time, just before humans targeted the mammals for whaling.
The carrying capacity of the North Pacific could have been as high as 170,000, "assuming modern day values for benthic productivity, food density, and gray whale energetics," the authors concluded. If gray whales also exploited non-benthic organisms, such as krill, the populations could have been even higher.
If gray whales do respond well to the rising temperatures and sea levels predicted for the future, that may not be true for the birds and other marine mammals that feed in the Bering Sea, one of the most productive marine ecosystems during the summer.
"If this environment disappears in glacial maxima, we really need to rethink what we know about the ecological history of all the other organisms that make a living in the Bering Sea," Pyenson said. He and Lindberg urge other scientists to focus on the historical ecology of species to fully understand their co
|Contact: Robert Sanders
University of California - Berkeley


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The Star-Advertiser Launches Monday But Hundreds Of People Still Don't Know Whether They Have Jobs - Posted June 3, 2010
The Honolulu Star-Advertiser, the paper created from the merger of the Honolulu Star-Bulletin and the Honolulu Advertiser, will launch June 7. But hundreds of staffers are still waiting for official word about whether they should report to work on Monday. Two dozen in the newsroom have reportedly received offers, including William Cole, Rob... [MediaJobsDaily]


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Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells
© Yu et al. 2009
Received: 17 May 2008
Accepted: 26 February 2009
Published: 26 February 2009
Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-ε-caprolactone (PCL) composite scaffolds.
Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel™ for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability.
The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group.
Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.
One approach to tissue engineering consists of seeding appropriate cells on a biodegradable scaffold, stimulating cell growth and differentiation in vitro, and then implanting the engineered complex in vivo to achieve functional tissue [1, 2]. However seeding a single cell type into a biomaterial scaffold to replace an injured tissue that consists of multiple cell types is usually inapplicable. An alternative strategy is the generation of a composite graft, which contains not only the tissue specific cell types, but also other supportive cells, such as endothelial cells (ECs) to promote vascularization of the grafts.
ECs may be incorporated into bioengineered tissue[3, 4]to promote the tissue revascularization, and transportation of oxygen and nutrients. Unfortunately, differentiated ECs isolated from most tissues including aortas, dermal capillaries and umbilical veins have inadequate proliferating capacities and are less responsive to angiogenic survival factors or anti-angiogenic signals [5, 6]. In contrast, bone marrow-derived endothelial progenitor cells (EPCs) possess high potential for neovascularization and reendothelialization [7]. EPCs isolated from bone marrow or peripheral blood have been observed to undergo more than 1000 division cycles [8], indicating that even the comparatively low numbers of adult EPCs may provide sufficient seeding cells for tissue engineering applications. Bone marrow stromal cells (MSCs) are multipotent stem cells originating from the bone marrow stroma, and represent a particularly promising cell source for bone tissue engineering. They can be easily harvested, expanded in vitro and induced to differentiate to bone-forming cells [9]. We have therefore selected MSCs as the source of osteogenic precursors for tissue engineered bone in this project. Polycaprolactone (PCL), an FDA-approved polyester commonly as drug delivery devices used in clinical practice, has been shown to be non-toxic to cells [10, 11], possessing many of the desirable properties such as degradability and plasticity. Hydroxyapatite (HA) is the inorganic part of the naturally occurring bone, and is known to be both biocompatible and osteoconductive. It suggests that the addition of HA to PCL will improve the biocompatibility and osteoconductivity of the polymer [12–14]. However, the precise dose-response relationship of HA in PCL on viability and osteogenic functions of bone marrow-derived osteoblasts remains to be elucidated. Although ECs-initiating vascularization in the engineered bone is critical [15], the survival and bioactivity of EPCs-originated ECs in biomaterials of bone graft is frequently neglected. Therefore, the objective of this study was to evaluate the biocompatibility of the HA-PCL biomaterials to both bone marrow-derived osteogenic and endothelial cells.
Cell Culture and Induction
Bone marrow cells were obtained from male BALB/c mice (6–8 weeks of age). Low-density bone marrow mononuclear cells (MNCs) were isolated by density centrifugation over Histopaque®-1083 (Sigma-Aldrich, US). Cells were then cultured in flask at 37°C and 5% CO2 atmosphere for differentiation of osteoblasts and endothelial cells, respectively. For osteogenic cell induction, cells were cultured in complete media [16, 17] consisting of DMEM supplemented with 10% fetal bovine serum (FBS) (Invitrogen, US), 10 mM β-glycerol phosphate (Sigma-Aldrich, US), 10-4 M L-ascorbic acid (Sigma-Aldrich, US), and 10 nM dexamethasone (Sigma-Aldrich, US), 2 mM glutamine (Invitrogen, US), 100 U/ml penicillin (Invitrogen, US), 100 μg/ml streptomycin (Invitrogen, US). To promote the endothelial phenotype of EPCs, the mononuclear cells were plated onto flasks coated with fibronectin (Sigma-Aldrich, US) and cultured in endothelial cell basal medium-2 (Cambrex, US) supplemented with EGM-2 MV SingleQuot® kit containing 5% FBS, human epidermal growth factor (hEGF), human vascular endothelial growth factor (VEGF), human insulin-like growth factor-1 (IGF-1), hydrocortisone, penicillin (Invitrogen, US), and streptomycin (Invitrogen, US). After 4 days of culture, non-adherent cells were discarded by washing with PBS. When 60% confluence was achieved, cells were subcultured.
Cell Characterization
Immunocytofluorescence studies were performed to detect the induced endothelial phenotypes. The induced ECs were fixed in 4% paraformaldehyde, permeated with 0.01% Triton X-100 in PBS, and incubated in 1% block serum for 1 h at 37°C. The cells were then incubated for 1 hour with monoclonal antibody against either mouse VEGFR-2 or mouse vWF (Santa Cruz, US). Bound antibodies were detected by incubation with fluorescein-5-isothiocyanate (FITC)-conjugated (Jackson ImmunoResearch, US) (for VEGFR-2) or Alexa Fluor 488-conjugated (Molecular Probes, US) (for vWF) secondary antibody. The cells were examined in fluorescence microscope. 300 μl of Matrigel™ (BD Biosciences, US) mixed with 4 × 104 EPCs-derived ECs at 4°C was dispensed into a 24-well plate and incubated at 37°C until solid. Photographs of capillary-like formation were taken at 7 days of culture in normal condition.
Similar fixation, permeabilization, and blocking processes were performed on bone marrow-derived osteoblasts, followed by the incubation with anti-osteocalcin (Santa Cruz) for 1 hour, and visualization was achieved using avidin-peroxidase complex (ABC kit, Santa Cruz Biotechnology, US). Cells were counterstained with Gill's hematoxylin solution. Calcium deposit produced by osteoblasts was demonstrated using von Kossa staining. After fixation in 4% paraformaldehyde, the cells were incubated with 1% silver nitrate solution (Sigma-Aldrich, US) under ultraviolet light for 20 minutes, and unreacted silver was removed by 5% sodium thiosulfate (Sigma-Aldrich, US). The alkaline phosphatase (ALP) activity of osteoblasts was assayed using an ALP kit (Sigma-Aldrich, US). The induced osteoblasts on slides were fixed in citrate-acetone-formaldehyde solution at room temperature for 1 minute. Following incubation in alkaline-dye mixture for 15 minutes and rinsing in distilled water, the slides were counterstained with hematoxylin solution.
Preparation of HA-PCL Scaffolds
PCL-HA scaffolds were prepared using a particulate leaching technique as described previously [18]. The HA-PCL composite at 2 different component ratios were prepared respectively, with HA (Sigma-Aldrich, US) to PCL (Aldrich, US) at 1:1 (Group A) or 1:4 (Group B) wt/wt. PCL scaffolds without HA were used as a control (Group C). In each group, NaCl particles (particle size 212–355 μm) were used to generate a controlled level of porosity in the matrix with weight ratio to PCL at 16:1 (Group A), 8:1 (Group B) and 4:1 (Group C). PCL (Mn 80000) was dissolved in tetrahydrofuran (Sigma-Aldrich, US) at 10% wt/vol for 12 hours. HA powder (≤ 40 μm particle size) and NaCl particles were mixed to homogeneity in the PCL solution, which was sonicated for 60 seconds until viscous slurry developed. Mixtures were poured into glass dishes to a thickness of 4 mm, and dried at 37°C. After evaporation of the solvent, 1.5 × 1.5 cm squares were cut out and washed in excessive distilled water to leach out the NaCl. All materials were then sterilized in 70% ethanol and dried before biological evaluation. Samples of the PCL-HA scaffolds were gold sputter coated and their morphology was observed using SEM (Hitachi S-2400, Japan) at 15 kV. Energy Dispersive X-ray (EDX) analysis was also conducted to confirm the existence of HA particles on the composite scaffolds. The atomic percentages of calcium and phosphorus were calculated.
Cells Culture on Scaffolds
Induced osteoblasts or endothelial cells in 50 μl suspensions (3.5 × 106 cells/ml) were respectively loaded onto each scaffold in 6-well plates. The scaffolds were left undisturbed in a 37°C incubator for 3 hours to allow cells to attach to the scaffold, after which the cells-materials complex were kept in culture using the original osteogenic or endothelial media. At day 7 the samples were harvested for and biochemical evaluation.
For morphological examination, cells-materials complex were fixed with 1.5% glutaraldehyde (Fisher Scientific, US) for 30 min at 4°C. The samples were exposed to 2% osmium tetroxide (Sigma-Aldrich, US) for 30 min. Following rinse in distilled water, they were dehydrated through a graded series of ethanol (50, 70, 90, and 100%) for 2–5 min. The dehydration was completed in hexamethyl disilazane (Fluka, Germany) for 10 minutes. After air-drying and sputter coating with gold, the cells morphology on the PCL-HA scaffolds was evaluated using SEM at 10 kV.
Assessment of Cell Viabilities and Functions on Scaffolds
For biochemistry assay, each type of cells was seeded on 30 scaffolds per group. Cell viability was evaluated by analyzing the mitochondrial activities of the cells. The Alamar Blue assay (BioSource, US) was used to determine the mitochondrial activity after 7 days of cell culture. The cells-materials complexes were washed in Phosphate-Buffered Saline (PBS) in 6-well plate. 3 ml of new conditioned media supplemented with 200 μl of Alamar blue was added to each well. Incubation was continued at 37°C, 5% CO2, for 3 hours. The culture medium was then transferred to a 96-well plate and read on a spectrofluorometer (excitation wavelength 530 nm, emission wavelength 590 nm). The Alamar blue absorbance/mg of DNA values was calculated for each sample.
Cell amount were determined by a fluorometric quantification of DNA in the cells-materials complexes. After the Alamar Blue assay, the cell-scaffolds were rinsed with PBS, followed by 4 times of freezing (-80°C) and thawing (37°C) cycles for 15 minutes each. The scaffolds were then homogenized in 1.4 ml of cold 10 mM EDTA solution (Sigma-Aldrich, US). The pH of the samples was adjusted to 7.0 by adding 1 M KH2PO4 prior to the addition of 1.5 ml of the 200 ng/ml Hoechst 33258 fluorescent dye (Sigma-Aldrich, US). 100 μl of supernatant sample were read with an excitation set at 350 nm and an emission at 455 nm on a spectrofluorometer. The DNA concentration in the samples was determined against a DNA standard curve that was plotted according to a series of 100 μl of calf thymus DNA (Sigma, US) in a range of concentrations from 0.15265 to 20 μg/ml. The DNA values were used to normalize the cell viability and other cell function parameters.
Production of ALP by osteogenic cells was measured using a spectrophotometer. After the previous freeze-thaw cycle and DNA assay, 50 μl of the sample was transferred to a fresh 96-well plate and 50 μl of p-nitrophenyl phosphate solution (Sigma, US) was added to each sample. Following incubation for 5 min at 37°C, the production of p-nitrophenol in the presence of ALP was measured by monitoring light absorbance at 405 nm. The measurement of the ALP assay was normalized against the amount of total DNA in each sample.
The nitric oxide generated by endothelial cells on scaffolds was assessed using the Nitric Oxide Colorimetric Assay kit (Calbiochem, Germany) in accordance with the manufacturer's instructions. The presence of nitric oxide in the culture media of endothelial cells-materials complex was determined by detecting the colored product spectrophotometrically. The absorbance was read at 540 nm. The nitric oxide concentrations for samples were calculated according to the standard curve. Cellular nitric oxide amount was normalized by total DNA each sample.
Statistical Analysis
All experiments were replicated three times to ensure the reproducibility, and all data was presented as the mean ± standard deviation. Single factor analysis of variance (ANOVA) with a post hoc LSD from SPSS™ (Student Version 10.0.5, Chicago, IL) was used to assess the statistical significance among groups, which was defined as p < 0.05.
Cells Culture and Induction
Cell Characterization
Cells Culture on Scaffolds
Bone tissues are essentially composite materials consisting of various components tissues with different structural arrangements and functions. Repairing bone defects using tissue engineered grafts involves the interplay of several variables, including scaffold materials with excellent biocompatibility, osteogenic cells capable of assembling the complicated and functional bone tissue, and an appropriate vascular bed to support the metabolic needs of new bone tissue. We have been developing a composite scaffold consisting of PCL and HA to enhance the biomechanical properties as the potential bone graft substitute [19]. Furthermore, following introducing MSCs-derived osteoblast (responsible for osteogenesis) and EPCs-derived endothelial cells (accountable for vascularization), we attempted in the current study to evaluate the biological impacts of various component ratios in PCL-HA scaffolds on the two types of cells.
Large numbers of MSCs-originated osteoblasts and EPCs-originated endothelial cells could be obtained in dish culture in vitro, making them possible to construct transplantable tissues scaffolds. It has been shown that in vitro stem/progenitor cells possess the capability of self-renewal and differentiation into organ-specific cell types [20]. Our results indicated that MSCs and EPCs could be harvested from mice bone marrow and differentiate into osteoblasts and endothelial cells respectively. Besides the expression of specific molecular markers like osteocalcin, VEGFR-2, and vWF, induced MSCs and EPCs functioned normally, as indicated by both fine crystals of ossification (comparable to natural bone mineral) and ALP activity in osteoblasts, and capillary-like formation by endothelial cells in Matrigel™. Due to the capacity of capillary formation, it was speculated that induced endothelial cells might participate or mediate the neovascularization in tissue engineered bone in vivo, which had been elucidated in a study [15]. It demonstrated that these differentiated bone marrow-derived endothelial cells and osteoblasts had the potential to create a tissue-engineered bone graft with microvascular network. Therefore, it is necessary to evaluated biological effects PCL-HA scaffolds on these cells seeded on it.
PCL and HA have been shown non-toxic and non-mutagenic biomaterials [11, 21–24]. In particular, the biocompatibility of HA due to its similarities to natural bone mineral have led to HA widespread use in bone reconstructive surgery. Degradation and metabolism of PCL was completed in vivo, and ε-hydroxycaproic acid and water were the only metabolites [22, 25]. Our study indicated that inclusion of HA into PCL significantly increased the mitochondrial activity and expression of ALP by osteoblasts in a dose-dependent manner, which is in agreement with previous studies [12, 26]. The SEM images demonstrated that bone marrow-derived osteoblasts were spread on the scaffold surfaces with exposed HA particles and synthesized extracellular matrix. Nitric oxide (NO) is a biologically active molecule in the maintenance of vascular homeostasis and predominately produced by endothelial cells. We examined the levels of NO in cell media and in cell-scaffold composite to confirm and quantify the function of bone marrow-derived endothelial cells. Our data showed that the addition of HA elevated NO production in comparison with the HA-free PCL scaffolds, suggesting that HA particles promoted functions of the endothelial cells. Although Pezzatini [27] reported excellent biocompatibility of HA nanocrystals for endothelial cells, cell viability experiments of this study resulted no difference among endothelial cell groups. The different culture conditions and cell origins, disparate HA dimensions, and biomaterials architectures between our and Pezzatini's investigations may partially explain the diverse outcomes.
In this study, adding of HA into PCL led to heterogeneous surface properties. Variety of HA ratio to PCL generated distinct exposure of HA particle and diverse topography, which have been evidenced by SEM images. The exposed HA particles were more predominated on the high HA ratio scaffold than those on the lower ones, where the HA free scaffold just exhibited smoother surface on the pore walls. It appears that the rough surfaces due to HA embedding provided more anchorage for cell process and spreading, adhesion and orientation. Therefore, osteoblasts and endothelial cells were inclined to attach to the rougher surface and appeared better viability and strong cellular phenotypes, which supplement the literature reports that cell number and attachment force were increased on textured polymer substrates [26, 28, 29]. Furthermore HA is a well known sorbent for molecules. Involvement of HA particles on the PCL surface may change the surface charge, reduce the hydrophobicity of PCL and promote the adsorption of proteins and other molecules from surrounding environment [30, 31]. In hard tissues, proteins such as osteopontin, bone sialoprotein, and osteocalcin were able to recognize HA through highly acidic domains, resulting in the attachment and distribution of osteogenic cells on the surface of protein-coated HA, and subsequently improving cell proliferation and differentiation, and promoting new bone formation [32–35]. Additionally the calcium ions released from the dissolution of HA were able to neutralize PCL acidic products, so the adverse response due to the PCL degradation could be overcame [14, 36, 37].
In conclusion, our data indicated that supplement of HA into PCL provided a compatible environment for osteoblasts and endothelial cells to replicate and function. The HA surface exposure accounted for the positive cellular responses. Optimal component ratio in PCL-HA scaffold could be selected in term of bioactivities of osteoblasts and endothelial cells. These outcomes would contribute to the construction of vascularized engineered bone in vitro and implantation in vivo.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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November 14, 2014 5:10 pm
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EDUCAUSE Library Items for Simulations
- The Connected Learning Environment
July 29, 2013
Connected learning environments are changing education, student engagement, and college completion. While e-learning often connotes delivery of information in a sequential, linear fashion, th…
- Game-Based Learning: Developing an Institutional Strategy
August 9, 2012
Games have become a central component of our culture generally, and they have begun to assume a role in higher education, though typically only on a small scale. Several key trends will drive the…
- Cultivating Change in the Academy: 50+ Stories from the Digital Frontlines at the University of Minnesota in 2012
July 9, 2012
Cultivating Change is an eBook designed to stimulate discussion about what's possible as well as generate new vision and academic technology direction. It serves as a means for faculty, ins…
- Games in Education: Realizing Opportunities and Overcoming Obstacles
December 5, 2011
The potential for games to augment traditional education and create entirely new learning opportunities is seemingly endless. But along with its new opportunities, educational gaming also …
- Assessing for Deeper Learning
October 20, 2011
A session at the EDUCAUSE 2011 Annual Conference
In the face of a rapidly changing and increasingly global workforce, organizations and educational institutions continue to stress the need for developing "deeper learning" skills such as…
- New ELI 7 Things... Explores Gamification
August 12, 2011
Gamification is the application of game elements in non-gaming situations, often to motivate or influence behavior. The rewards or the spirit of competition can spur students' concentr…
- 7 Things You Should Know About Gamification
August 10, 2011
Gamification is the application of game elements in non-gaming situations , often to motivate or influence behavior. The rewards or the spirit of competition can spur students' concentratio…
- Hands-On Engagement Through Simulations
February 22, 2011
A session at the EDUCAUSE West/Southwest Regional Conference 2011
This special "Experience IT" session is designed to offer a hands-on, highly interactive introduction to an emerging tool and explore its potential impact for professional development and…
- Simulation Technologies in Higher Education: Uses, Trends, and Implications
February 9, 2010
This ECAR research bulletin focuses on the rapid growth in the use of simulation technologies in higher education and the implications this will have for information technology planning and poli…
- Expanding Educational Realities: Exploring Interactive and Immersive Learning Experiences
January 20, 2010
A session at the ELI Annual Meeting 2010
Today's students are increasingly choosing to move beyond the traditional physical classroom educational model. The Internet has created an educational evolution fostering learner-centered env…


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Searched for: "request to reschedule meeting"
Content Types
About 8 results for "request to reschedule meeting"
X.ai eliminates the back and forth of scheduling meetings through artificial intelligence
X.ai's personal assistant. brings artificial intelligence to the tedious task of scheduling meetings.
10 ways to reduce interruptions and stay focused at work
Interruptions are a fact of life, but they don't have to derail your work. These practical suggestions will help you maintain control of your day.
Consultants: It's not the theory, it's the execution
Project success has less to do with the adoption of a specific methodology or theory and more with how well the people involved can adapt the procedures to new challenges. Chip Cam...
Preparation, time buffers help prevent project bottlenecks
Predicting and preventing bottlenecks is like playing chess--you're wise to think two or three steps ahead. Check out some examples of projects whose progress was helped by anticip...
Buy some time by automatically scheduling administration chores
Don't waste your hard-earned certifications by spending your workdays doing tedious and mindless tasks. In this Daily Drill Down, Derek Schauland shows you how to use the Task Sche...
Built to last: Calling it quits
In spite of everyone's best efforts, some team members inevitably move on. Make the departure as beneficial as possible for everyone concerned by following these suggestions for th...
Diary of an IT director: Tom Ranalli (Thursday)
In this installment of his daily IT diary, IT Director Tom Ranalli meets with an IT business consultant to discuss ROI. Later, he wrestles with a redesign of his company's Web site...
The art of asking for a favor
IT managers who are overworked and understaffed may find the help they need just by asking. This week's installment of Artner's Law explains how to avoid the pitfalls when asking f...


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Fletcher Hotel Paleis Stadhouderlijk Hof
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Travel Blogs from Leeuwarden
Mass Trash Leeuwarden
... what looked even leaner with every step I took. I found it fascinating, a building what looks like it is going to collapse although this building is taking probably hundreds of years before it actually decides to drop. My thoughts about the Oldehove immediately destroyed when a young fellow pulls my sleeve. In his hands he holds a giant board in which numerous fake sunglasses and says that I need to buy sunglasses. Where the heck did ...
... Again so mild at 7:30 at night outside. Then we went home. Aunty Hennie is marvellous with all the driving she does at her age. Spent evening packing. Whatsapped Willow. Then unbelievably she Skyped ! It was so wonderful to see the girls. They were eating breakfast and were well behaved. They got to say hello to Aunty Hennie also. And the dog put in a great show which they loved. So I got to see all my family today except Johnny. Up ...
Groningen, Holland
... and BC ladies. The twin girls then went off to the shop on their own to get ice creams. Another drink then we left at 3:15. Glad to get off the fast motorway (120 k) and back on the country roads. Windmills, canals and all ages of people on bikes. Got in and I slept nearly an hour. Indonesian for dinner. Hennie is such a good cook, and she makes everything from scratch. Went for a short walk in the garden with the dog. Tired ...
Groningen, Holland
... as he'd had a car accident. Aunty Hennie ( mine now she said ! ) made another delicious dinner. Have been re inspired for when I get home, with cooking, having friends round, and making a cottage garden. Not in much pain for the evening. Angelique rung to say she fell off her bike into a ditch and her bike landed on her. She was covered in slime. Henk had to jump in and rescue her !!! Not nice but funny ...
Groningen, Holland
... Tired and emotionally spent for awhile. Nice talking to Tante Hennie. She seems to understand and it's nice not to be alone. James goes to Oslo today. So hard not being able to communicate with my family. Delicious meal, beautifully presented again. Watched a programme on finding lost great works of art in the UK. Freezing cold. Socks to bed ...


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Benfica came from behind to take a 2-1 first-leg lead against 10-man Liverpool in their Europa League quarter-final clash. More
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The Titanic Quiz BookAndrews UK 2012; US$ 3.99
Are you interested in the history of the Titanic? Would you like to find out more about this famous liner and how it came to sink? If so, The Titanic Quiz Book has all the answers.Few events in history have captured public imagination to such an extent as the story of the Titanic which has spawned numerous films, documentaries and books through the... more...
Learn on the LooMichael O'Mara 2015; Not Available
At home or in the office, the loo is one of those few places where we have space to sit and think. And now you can have your own private lavatory lecturer help you make the most of it. Featuring mind-expanding trivia, extraordinary facts and enlightening stories from history, science, the world of words and much, much more, Learn on the Loo provides... more...
The People's Quiz Book 2007Transworld 2011; Not Available
The People's Quiz 2007 is set to take the nation by storm. Over 12 consecutive weeks on Saturday nights, BBC 1 will feature the biggest quiz show the corporation has ever envisaged - with more questions, more contestants and a bigger prize fund than ever before. Regional auditions are open to the entire nation. Thousands have flocked to quiz... more...
Bamber Gascoigne's Challenging Quiz BookPenguin Books Ltd 2007; US$ 12.78
Who was the only British Prime Minister to be assassinated? The demolition of the old London Bridge put an end to what famous tradition? Which philosopher caught a fatal chill returning from a predawn lesson given to Queen Christina of Sweden? What was the real name of Buffalo Bill? Which mutiny, leading to a war of independence, was provoked by... more...
The Mastermind Quiz BookEbury Publishing 2012; US$ 15.98
Mastermind is the ultimate test of knowledge, memory and nerve. Over four decades hundreds have tested their mettle in the famous black chair, and dared to put their intellect under the glare of the Mastermind spotlight. Now, can you rise to the challenge? The Mastermind Quiz Book poses over 3,500 questions, from fiendishly difficult... more...
The Best of Bournvita Quiz ContestPenguin Books Ltd 2011; Not Available
It started as a radio quiz programme in 1972 and went on to become one of the most sought-after school quizzes in the country. On overwhelming public demand, BQC makes a comeback on television in 2011 and, with it, Quizmaster Derek O?Brien brings you a compilation of a thousand questions from the archives of India?s longest running television game... more...
The Fulham Quiz BookAndrews UK 2012; US$ 5.49
Is Fulham your local football team or the one you have chosen to support? Do you go to every match? Are you clued up on all the events, people and places that have shaped the clubs long history? Then why not find out how much you really know about The Cottagers with this fun quiz book?With 250 thought-provoking questions about all aspects of... more...
Collins Quiz Night (Difficult)HarperCollins Publishers 2012; Not Available
Over 1000 difficult questions from Collins Quiz Night to scramble even an egghead?s brains. Organised into over 60 separate quizzes covering everything from pop stars to death stars. Specially designed for e-readers, with easy-to-navigate question and answer sections so anyone can be the quizmaster. more...
Collins Pub Quiz (Difficult)HarperCollins Publishers 2012; Not Available
Over 1000 difficult questions from Collins Pub Quiz to scramble even an egghead?s brains. Organised into over 60 separate quizzes covering everything from pop stars to death stars. Specially designed for e-readers, with easy-to-navigate question and answer sections so anyone can be the quizmaster. more...
101 Interesting Facts about SpursAndrews UK 2012; US$ 2.99
Are you a football fan? Do you support Tottenham Hotspur FC? Would you like to find out more about the clubs long and successful history? If you answered yes to any of these questions, you are certain to enjoy this informative Spurs reference guide.Did you know that Tottenhams record cup win is 13-2, in an FA Cup game against Crewe during... more...


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Fredonia, NYUSA
Hometown: fredonia
January 3, 2012
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text: iPhone App 4/16/10 Download the PopSugar Celebrity Faceoff App For iPhone and iPod Touch Read More iPhone AppAside POPSUGAR, the #1 independent media and technology company for women. Where more than 75 million women go for original, inspirational content that feeds their passions and interests. Get Inspired With Our Living Newsletter Personalize Home Tech DIY Food Love and Sex Vegetarian Sign up with facebook or By signing up, I agree to the Terms and to receive emails from POPSUGAR.


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I received an email today telling me that the affiliate program for a shop I promote on one of my sites will be cancelled and closed, after October 31st affiliate commissions will no longer be paid out for referrals. This is a huge headache and … [Continue reading]
Affiliate Program Being Cancelled and Deactivated – Grumble, Sigh, Grrr
Adding New Niches To Old Niche Sites
I've always been one of those people that agree with the logic that you should do something you already know relatively well and enjoy OR pick a topic that you've always wanted to learn more about or expand upon. I learned the hard way that it makes … [Continue reading]
Finding Something You Can Write About Forever, and Ever, and Ever…
When people are talking about choosing a niche you're passionate about they always say things like you should pick something you could talk about all day, or pick the thing that you sit and talk about with friends all the time, or something people … [Continue reading]
Breathing New Life into Old Niches
a guest post by Gabrielle Greene Life is a funny thing; you never know where it's going to take you. When I got pregnant with my third child I made the decision to quit working outside the home, which at the time, was for a company that organized … [Continue reading]
Traffic and SERPs Tanked on Niche Site B – How Long is Too Long to Wait for a Recovery?
I had managed to rank in the top 10 results for a handful of keywords that were bringing in traffic and making sales on the site. Then suddenly on Thursday it all tanked. It doesn't appear to have received a "penalty," but I don't know why the … [Continue reading]
No New Squidoo Lenses in June
This is the third month in a row with no new Squidoo lenses. I believe that's the longest I've gone without putting something on Squidoo in the past five years. Even when I wasn't actively using Squidoo, I would put up a page to try and new niche or … [Continue reading]


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When Google unveiled its smart and controversial eyewear three years ago, some early tech adopters tried to do their part by eagerly pushing for Glass acceptance. The world pushed back.
You might think you're seeing a virtual tour of a real apartment in Paris, but it's actually a complete fabrication, created on a computer. This little CG apartment might seem like no big deal, but it's a huge testament to what's possible with the newest version of Unreal Engine, and we're excited.
On today's show, we give kudos (and chuckle a bit) at one man's attempt to make a mechanical horse at home, check out a jaw-droppingly realistic render from Unreal Engine 4 and discuss an inflatable planetarium with laser-projected stars inside.
Visual artist re-creates digital treasure from 1998 N64 classic in high-def glory using Epic's most powerful game engine.
One Star Trek fan and 3D artist is re-creating the entirety of the Enterprise-D from "Star Trek: The Next Generation" in the Unreal Engine 4 that can be explored with the Oculus Rift VR headset.
Visual-effects artist Michael Eurek builds an absolutely incredible high-definition remake of Zora's Domain from Legend of Zelda: Ocarina of Time using Unreal Engine 4.
At the 2014 Game Developers Conference, Epic Games' Tim Sweeney announces that one of the most powerful engines in game design is now widely available.
Find out what's new and what's changing here in patch 1.05.
"We'd need a really great partner."
In the next installment of the CraveCast, we just can't wait for Halloween to talk all things scary and unreal. Someone will be in costume, so join us at noon PT on Tuesday.