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When a person acts or speaks in a rhetorical manner reminiscent of Karl Rove.
When Hillary Clinton warped what Barack Obama said about the Reagan era, she used Rovean tactics.
by praxguy February 07, 2008
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ALL SKY: If Sky Ferreira comes to Coachella next month, Forever 21 has her wardrobe ready. The New York-based pop star is the face of the retailer's 2013 capsule festival collection in sunlight-drenched images shot by David Roemer at The Saguaro hotel in Palm Springs, Calif. The collection has 85 pieces priced from $4.80 to $39.80 featuring dip-dye denim, lace, studs, crochet, and vivid colors and patterns. The ads launch March 14, and the capsule festival collection will be in stores March 22. Ferreira isn't a stranger to fashion ads. She has been in campaigns for the likes of Calvin Klein, Adidas and Vigoss.


text: Idril NSFW Safe Is this playlist safe for work? gold He was a refined piece of bad news June 08, 2012 | 28min 8 tracks | 984 142 | Stats Nicolas JaarJohn MurphyKiEw experimentalexperimental electronicpipe smokingdark ambientragtime through a locked door Liked Collect Share Edit Liked! Share this mix with friends:


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My credit score is low due to:
-- Delinquency of accounts (some 30's plus one 60 last year), though I have at least 8 months of steady, on-time payment of 3 small cards (min. utilization) and an auto loan.
--Midland reviving zombie debt (I have clear case regarding DoFD, so very encouraged there will be an out here)
--Asset Acceptance did the same, though currently, mid-validation, they've ceased to report. I do not know yet if this is a permanent or temporary reprive. We'll see!
--"Excessive inquiries" or hard pulls, currently at 8
I'm going to get Midland off, one way or another, because I have VERY clear documentation that the DofFD is WAY off (by several years). Asset Acceptance *may* be gone, my newest credit (cards and car loan) has strong on-time history beyond that small period about 12 months ago (can I goodwill any of those older lates?). This leaves the hard pulls. Has anyone challenged hard pulls before? I feel I have a case.
Of 8 pulls on my TU report FIVE are related to one deceptive situation at an auto dealership. And, there are additional companies related to that same incident who reported to other CRAs. Basically, I applied for Capital One auto loan, got it, and had a blank check for shopping dealerships. Once I decided on the car I wanted, I went to purchase from a dealership who lied to me about certain things I was signing, including the credit "authorization." When I said I did not want a credit pull, I did not want dealership financing & I would be using my own financing, I was told that was a document related to the car registration. All said and done, they shopped it, without my consent, to a TON of companies & now my "excessive inquiries" are primarily due to that--all occuring on that one evening with creditors who sent me letters refering to the dealer. I left the dealership, without the car, by the way, immediately made complaint to the dealership & the whole transaction was canceled.
I spoke with the CRAs regarding what would be needed to dispute these hard pulls. I do have a chain of emails regarding my complaint, wherein the credit misrepresentation was key & their promise not to send my information out regarding any further financing the *day after.* But, CRA says they would like to see a letter from the company saying that I didn't authorize. The pulls are from the dealership and a bunch of other entities -- Wells, Chase, etc. I figured if the dealership would provide a letter, the other lenders can just confirm they got the information from the dealership. So, I started with a gw request to the dealership for a letter like the CRA wants. They spoke with me, discussed it internally, and responded that it would be "improper" to provide such a letter when they have a credit authorization on file. I just shot off a letter back to them, to clearly make my case. (I'll post in a seperate post below for anyone interested)
Let me know if you know of people who have got hard pull deletions, also any good will removals of old delinguents on an otherwise good standing account?? Brought my score from 540 to 593 & with a goal of 640 within the next 6 months! Need to deal with this stuff!
I would have contacted a local news reporter .... newspaper or TV ... by letter and cc the dealership's general manager. I would have followed up with an ad in my local newspaper as well.
11-14-2013 07:43 AM - edited 11-14-2013 07:44 AM
I would send a formal complaint to the CFPB for their violation of the FCRA. They have authority to issue sanctions for clear violations of the FCRA.
You could additionally send a complaint to your local BBB based on their apparently deceptive business practices.
They did not have permissible purpose under FCRA 604 unless they had a legitimate business need to review your credit as part of your transaction with them.
if not seeking financing, they would have no basis to reivew your credit.
That assumes that you did not sign an authorization for them to pull your credit. If you gave express authorization, that trumps any need for them to provide a permissible purpose.
RobertEG wrote:
I would send a formal complaint to the CFPB for their violation of the FCRA. They have authority to issue sanctions for clear violations of the FCRA.
You could additionally send a complaint to your local BBB based on their apparently deceptive business practices.
They did not have permissible purpose under FCRA 604 unless they had a legitimate business need to review your credit as part of your transaction with them.
if not seeking financing, they would have no basis to reivew your credit.
That assumes that you did not sign an authorization for them to pull your credit. If you gave express authorization, that trumps any need for them to provide a permissible purpose.
This is true. However, it seems that there are more and more dealerships that are putting that authorization in a pile of paperwork and having you sign it. Even not clearly stating what it is for. In one case, the pulls were done days after the vehicle was purchased through outside financing.
That is deceptive practices and they should be held accountable.
If you are not seeking financing there is no reason for them to pull your CR. Period. Or to lie about it either.
Thanks for all the good ideas. I do have a dynamite consumer advocate who was helping me directly after the incident first occurred (to make sure I wasn't stuck with the car), so I can definitely add that to my leverage.
The problem is that I did sign a credit authorization, but under a DIRECT misrepresentation. It wasn't that it was hiding in a pile of papers, but that I said, "I don't want financing, I don't need you to pull my credit, I'm using the financing I have." They put the paper in front of my face and told me I was signing something that was "part of the vehicle registration process." Later, they directly lied about other things they asked me to sign as well. They lied and said they were using my pre-approved financing, too. I walked out without the car, reviewed the paperwork, and immediately shot off a communication to the internet sales guy I had been dealing with, then talked to/emailed the manager first thing the following day. My complaint from the minute it happened was documented. I signed things, including the credit authorization, based on misrepresentations ... while being very clear verbally throughout my visit about my intent.
It was my first time buying at a dealership. I shopped online until satisfied & went to the dealer to just pick up the car & finalize the deal. I expected a hard sell, but not outright lies. Also, I had a minor daughter waiting for me and was in extreme stress about time. They knew that. Told me before I went that I could be in and out in 15-20 minutes. I learned my lesson about how to handle a dealership (like NOT going in when time-stressed) and did buy the exact same make/model car elsewhere without issue a few weeks later. I believe the misrepresentation means I authorized nothing. And, I certainly had no intention to authorize any such thing.
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After raising rates last year, the streaming-video service ups the price of its "standard" option in the US. But current members won't see a higher bill for a year.
From the Cheapskate: This 8-inch tablet is a far better deal than Amazon's new Fire HD 8. Here's why.
Are you a bit of a shutterbug? Add that to the list of reasons to avoid the 16GB model of the latest iPhone.
Technically Incorrect: He might pose as a tech expert. But even the best make mistakes. Having signed up for Twitter, Edward Snowden gets buried in e-mail notifications from followers.
A draft of the country's encryption policy would have made it mandatory for residents to keep all encrypted data, which includes Gmail and WhatsApp, for 90 days.
Brian Cooley answers viewer email and tells you what to think about when making the choice between a hybrid or electrified vehicle.
A Flipkart exec calls the email -- which states that "beautiful women are more successful in their lives" -- a "big fail" and promises to investigate the matter.
Technically Incorrect: A patent application filed by Microsoft describes giving your skin a little buzz when you get a notification.
This new email app uses swipes, taps and flicks of the wrist to turn clearing your inbox into a game.
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MAC Lustre in Jubilee
170 reviews
84% would repurchase
Package Quality: 4.4
Price: $$$
Package Quality: 4.4
Price: $$$
on 10/30/2004 12:23:00 PM
Age: Unknown
Skin: Combination, Other, Not Sure
Hair: Blond, Other, Other
Eyes: Brown
I love this color & keep it in my bag always. It's my default color, seems to go with everything. Perfect neutral for my blond hair, brown eyes & nc20 coloring.
on 10/13/2004 9:23:00 PM
Age: 25-29
Skin: Combination, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Brown
Fabulous color! Perfect nude shade without too much peach. Probably better than any nude color I've tried. Would surely recommend and re-purchase.
on 10/11/2004 4:42:00 PM
Age: 19-24
Skin: Normal, Fair, Cool
Hair: Blond, Curly, Fine
Eyes: Blue
this is my favorite nude ever. I'm a PPP and generally I cant do nudes because they make me look like I dont have any lips. this lipstick has enough of the strong but light pink brown color to give me a nude that actually looks right. I pair this with smokey eyes for a dramatic look and I can use Jubilee for a nice everyday lipstick.
on 5/22/2004 1:34:00 PM
Age: 36-43
Skin: Combination, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Green
The perfect slightly peachy nude worn on my medium mauvy pink lips. Against my NC/NW 15/20 skin it is a not to light and not to dark color, that harmonizes well with the peachy/orangey eyeshadows I favor, complimenting my look without drawing attention to itself. The lipstick itself is slighly drying and I prefer to wear it over a lipbalm which gives a slightly sheerer more natural look. It wears acceptably.
on 5/17/2004 1:12:00 AM
Age: 19-24
Skin: Other, Other, Not Sure
Hair: Other, Other, Other
Eyes: Other
Jubilee is a more neutral version of Hug Me: a medium beigey-pink. As much as I like the Lustre lipsticks in theory, my lips are pigmented, which means I need a base (like Subculture lipliner) for this to last for more than five minutes without my natural lip colour peeking through. Even then, the staying power isn't great. But the colour goes with just about anything, which is why I let it hang around.
on 5/8/2004 12:33:00 AM
Age: 19-24
Skin: Combination, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Other
this looks light pinky peach beige on my lips..haha.sorry for the sucks color description...i like any nude lip color^^
on 5/5/2004 4:59:00 PM
Age: 25-29
Skin: Normal, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Brown
This is one of my favorite nude lippies! I'm an NC30 with highly pigmented lips and this looks great by itself or topped with gloss. For me, it's one of those l/s I can apply without really looking! I love it!
on 3/28/2004 2:40:00 PM
Age: 44-55
Skin: Oily, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Brown
Love this...I have found the purrrrrrrrrfect nude-pick lipstick to put on daily...even looks better added w/ the clear lipglass for nights out...big rave!
on 3/15/2004 5:09:00 PM
Age: 18 & Under
Skin: Combination, Other, Not Sure
Hair: Brunette, Other, Other
Eyes: Hazel
Okay, I love this color. This is my perfect neutral l/s, light pinky beige, but why, oh why, is this so drying? That is why it gets three lippies but I would love to give it more if it was more conditioning. Oh well! I would still rebuy it b/c it still gives good coverage and nice pigmentation.
on 2/18/2004 4:55:00 PM
Age: 25-29
Skin: Other, Other, Not Sure
Hair: Other, Other, Other
Eyes: Other
This is really a daytime lipstick...I really like to use it for work or for an sat afternoon outing. I have an olive complexion and dark brown hair, so this color warms and brightens me up a bit, esp on tired mornings! I would give it 5 lippies but it does fade off fairly easily, forcing you to reapply all the time.
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When a distress call draws the Doctor and Sarah Jane to a scientific outpost at the end of the Universe, the Doctor suspects dark planetary forces are behind a series of sinister deaths.
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One Step closer to completing my collection. Thank you, it's a great story!Published 3 months ago by VictorSlim3D
The Planet of Evil sounds rather alarming, but it is my three year old's favorite Dr. Who (even more that the Robot one). Read morePublished 4 months ago by Patricia B.
Excellent product and service. Best TV show after all time. Tom baker best ever dr. Who.Published 8 months ago by Sean powell
My Son Loves the Dr!
He is a true Whovian! If that is a word! LOL and this episode is a good one! I am a fan of Dr. Who Number 4 too however my son can tell you ALL!
one of the true classic stories. tom baker and elizabeth sladen are at their best.Published 11 months ago by Mark Anderson
The story makes you think how hard they had to do to make the show so sci-fi back then. They did not have all of the graphic design stuff like we got in this world right now. Read morePublished 12 months ago by Jason Rayle


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Liver Biology/Pathobiology
Circulating microRNAs in exosomes indicate hepatocyte injury and inflammation in alcoholic, drug-induced, and inflammatory liver diseases†
Article first published online: 26 JUL 2012
DOI: 10.1002/hep.25873
Copyright © 2012 American Association for the Study of Liver Diseases
Volume 56, Issue 5, pages 1946–1957, November 2012
Additional Information
How to Cite
Bala, S., Petrasek, J., Mundkur, S., Catalano, D., Levin, I., Ward, J., Alao, H., Kodys, K. and Szabo, G. (2012), Circulating microRNAs in exosomes indicate hepatocyte injury and inflammation in alcoholic, drug-induced, and inflammatory liver diseases. Hepatology, 56: 1946–1957. doi: 10.1002/hep.25873
- †
Potential conflict of interest: Nothing to report.
Publication History
- Issue published online: 31 OCT 2012
- Article first published online: 26 JUL 2012
- Accepted manuscript online: 9 JUN 2012 10:51AM EST
- Manuscript Accepted: 21 MAY 2012
- Manuscript Received: 25 JAN 2012
Funded by
- NIAAA. Grant Numbers: AA017729, AA020744, DK075635
MicroRNAs are fine tuners of diverse biological responses and are expressed in various cell types of the liver. Here we hypothesized that circulating microRNAs (miRNAs) may serve as biomarkers of liver damage and inflammation. We studied miRNA-122, which is abundant in hepatocytes, and miR-155, -146a, and -125b, which regulate inflammation in immune cells in mouse models of alcoholic liver disease (ALD), drug (acetaminophen, APAP)-induced liver injury (DILI), and Toll-like receptor (TLR) 9+4 ligand-induced inflammatory cell-mediated liver damage. We found that serum/plasma miR-122 correlated with alanine aminotransferase (ALT) increases in the liver damage caused by alcohol, APAP, and TLR9 (CpG)+4 (LPS) ligands. MiR-155, a regulator of inflammation, was increased in serum/plasma in alcoholic and inflammatory liver injury. Alcohol failed to increase serum miR-122 in TLR4-deficient and p47phox-deficient mice that were protected from ALD. We found the most robust increase in plasma miR-122 in DILI and it correlated with the highest ALT levels. Consistent with the massive inflammatory cell infiltration in the liver, plasma miR-155 and miR-146a were significantly elevated after CpG+LPS administration. We show for the first time that, depending on the type of liver injury, circulating miRNAs are associated either with the exosome-rich or protein-rich compartments. In ALD and in inflammatory liver injury, serum/plasma miR-122 and miR-155 were predominantly associated with the exosome-rich fraction, whereas in DILI/APAP injury these miRNAs were present in the protein-rich fraction. Conclusion: Our results suggest that circulating miRNAs may serve as biomarkers to differentiate between hepatocyte injury and inflammation and the exosome versus protein association of miRNAs may provide further specificity to mechanisms of liver pathology. (HEPATOLOGY 2012;56:1946–1957)
MicroRNAs (miRNAs) are a class of RNA molecules (18-21 nucleotides) that do not encode for any protein but regulate ≈30% of total genes in the body.1 After their discovery in 1993, various roles of miRNAs have been described in development, immunity, stem cell differentiation, and cancer.1, 2 Recent studies suggest that miRNAs are not only localized within the cell (cellular miRNA) but are also present in circulation, making them attractive for biomarker discovery.3
Various agents ranging from chemicals to viruses trigger liver damage that is clinically evaluated by biochemical markers, including serum aminotransferases (ALT and AST). However, there are several limitations of these clinically utilized markers of liver damage, including requirement of fresh blood samples, lack of tissue specificity, and inability to distinguish between hepatocyte damage and inflammation—two important determinants of liver pathology leading to chronic liver disease. Circulating miRNAs possess several advantages over current clinical "standards." MiRNAs have tremendous stability in extreme conditions (low pH acidic environment, RNase resistance) and are considered alternative noninvasive biomarkers.3, 4 Moreover, various disease-specific circulating miRNA signatures have been reported in recent years.4, 5
MiR-122 is abundant in hepatocytes and recent studies have demonstrated that increased circulating miR-122 is associated with hepatitis C virus (HCV) infection, hepatocellular carcinoma (HCC), acetaminophen (APAP), and acute alcohol-induced liver injury.6-8 MiR-122 has diverse functions in hepatocytes, of which regulation of cholesterol metabolism is highly relevant to liver steatosis.9 MiR-155, a master regulator of inflammation, is up-regulated in inflammation-related diseases.10-12 MiRNA-155 targets multiple components of the proinflammatory cytokine production cascade, Toll-like receptor (TLR) signaling, and intracellular signaling pathways determining pro- and anti-inflammatory cytokines.13 MiR-146a and miR-125b are also involved in regulation of inflammation.13
The extent of hepatocyte damage and inflammation can differ between various etiologies of liver disease and may also change during the course of chronic liver disease. For example, prolonged/chronic alcohol induces fatty liver and additional inflammation promotes progression of chronic liver disease.14 APAP-related drug-induced liver injury (DILI) is characterized by massive and coordinated hepatocyte death and little inflammation.15 In contrast to APAP-induced DILI, liver damage induced by endogenous and exogenous danger molecules such as TLR9 ligand (unmethylated DNA rich in cytidine-phosphate-guanosine [CpG motif]) and TLR4 ligand (lipopolysaccharide [LPS]) results in robust inflammation and liver injury in mice.16
In this study, we hypothesized that circulating microRNAs could be markers of liver damage and/or inflammation. We tested circulating miR-122 as a potential marker of hepatocyte damage and miR-155 as a marker of inflammation in different forms of liver injuries induced by alcohol, acetaminophen, and CpG+LPS treatment. Our data show that increases in circulating miR-122 and miR-155 correlate with liver injury and inflammation, respectively. Furthermore, we show for the first time that the association of miRNAs with exosomes could be characteristic for the type of injury causing liver damage.
Materials and Methods
Additional methods are provided in the Supporting Information.
Animal Studies.
Mice were housed in the University of Massachusetts animal facility and cared for in compliance with the protocols approved by the Institutional Animal Use and Care Committee of the University of Massachusetts Medical School.
Serum and Plasma MicroRNA Analysis.
Prior to total RNA isolation, equal volumes of plasma or serum samples were thawed on ice, mixed with QIAzole (Qiagen), vortexed, and incubated at room temperature for 5 minutes. Synthetic Caenorhabditis elegans (cel)-miR-39 was spiked and after this step miRNeasy kit protocol was followed as per instructions (Qiagen). TaqMan miRNA Assay (Applied Biosystems) was used to analyze the miRNA from serum or plasma samples. Cel-miR-39 was used to normalize the technical variation between the samples.
Exosome Isolation.
One hundred microliters of plasma/serum was mixed with ExoQuick exosome precipitation solution and exosome isolation was performed in accordance with the manufacturer's protocol (SBI System Biosciences cat. no. EXOQ5A). Briefly, after incubation at 4°C for 30 minutes the samples were centrifuged at 1,500g for 30 minutes. The supernatant was denoted the protein-rich fraction, whereas the pellet was denoted the exosome-rich fraction. The exosome fraction was washed twice with phosphate-buffered saline (PBS) and lysed with QIAzole (Qiagen) or NP-40 buffer (1% NP-40, 150 mM NaCl, EDTA plus protease inhibitors) for total RNA or protein isolation, respectively. Of note, in our study we expect microparticles (≈200-1,000 nm) to be in protein-rich fraction, as the exosome precipitation solution precipitates up to 90 nm size particles.
Statistical Analysis.
For correlation analysis, Pearson's correlation test was performed in GraphPad Prism software. The nonparametric Mann-Whitney test or two-tailed t test was employed for statistical analysis. P < 0.05 was considered statistically significant.
Increased Circulating MiR-122 and MiR-155 in Alcoholic Liver Disease.
Alcoholic liver disease (ALD) is characterized by fat accumulation and activation of the proinflammatory cytokine cascade in the liver.14 Alcohol-fed mice showed significant fat accumulation in hepatocytes, mild necrosis and inflammatory infiltrate, and increased serum ALT compared to isocaloric diet-fed controls (Fig. 1A,B, left panel). To test the hypothesis that circulating miRNAs may serve as biomarkers of liver injury, we evaluated miR-122 that is uniquely abundant in hepatocytes. We found a profound increase in serum miR-122 levels in alcohol-fed mice (Fig. 1B, right panel). Furthermore, a positive linear correlation was present between serum ALT and serum miR-122 levels (Fig. 1C).
Because the distribution of miRNA may change during serum collection,17 we evaluated miR-122 levels both in serum and plasma fractions in mice after alcohol feeding and found comparable levels (Supporting Fig. 1A,B). We also found that the extent of alcohol-induced increase in miR-122 was similar regardless of the internal controls (miR-16 or miR-223) in both fractions (Supporting Fig. 1.A-D). These results suggested that chronic alcohol consumption resulted in miR-122 increase in the circulation.
Alcohol consumption not only causes fat accumulation in the liver but also results in inflammation via induction of proinflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-1β.14 Recently, we reported that alcohol increases miR-155 in macrophages, regulating TNF-α production.18 Consistent with this, TNF-α levels were increased in the liver of alcohol-fed mice (Fig. 1D). Furthermore, we found increased serum miR-155 levels in alcohol-fed mice (Fig. 1E). Serum levels of miR-125b and miR-146a that also regulate inflammation were not significantly changed (data not shown), suggesting that alcohol-induced liver inflammation correlates with increased serum miR-155 levels.
TLR4 Deficiency or Disruption of Oxidative Stress Prevents Increased Circulating MiR-122 in ALD.
LPS, a TLR4 ligand, plays a key role in the pathogenesis of ALD. Previously, our laboratory demonstrated the critical role of TLR4 in ALD and showed that TLR4-deficient (TLR4KO [knockout]) mice were protected from ALD.19 Because we found increased serum miR-122 in alcohol-fed mice, we hypothesized that miR-122 increase correlates with liver damage rather than the alcohol intake itself. We found that wildtype but not TLR4KO mice showed significant liver damage, steatosis, and serum ALT increase after alcohol feeding (Fig. 2A,B) and, importantly, there was no increase in serum miR-122 induction in TLR4KO mice after alcohol feeding (Fig. 2C). These results imply that serum miR-122 increase after alcohol administration occurred only in the presence of liver damage.
Increased reactive oxygen species (ROS) generated after alcohol metabolism or LPS contribute to the pathogenesis of ALD. Specifically, the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) oxidase enzyme complex plays a role in ROS generation in ALD and mice deficient in the p47phox subunit (p47phoxKO) of NADPH oxidase are protected from alcoholic liver injury.20, 21 Therefore, we next evaluated the role of ROS in the induction of serum miR-122 levels. Histopathological and biochemical studies indicated no significant fat accumulation or ALT increase in p47phoxKO mice compared to wildtype mice after alcohol feeding (Fig. 2D,E). Although alcohol-fed wildtype mice showed significant up-regulation of serum miR-122, there was no significant increase in serum miR-122 in p47phoxKO mice compared to pair-fed controls (Fig. 2F), implying that ALT and serum miR-122 levels mirror one another and serum miR-122 could be a marker of liver damage in ALD.
APAP Administration Results in Increased Circulating MiRNA-122, -155, -125b, and -146a Levels.
APAP-induced acute liver failure is a significant cause of DILI and mortality worldwide.22 ALT and AST are routinely used methods to assess liver damage after APAP overdose and here we evaluated the utility of circulating miRNAs. APAP administration (500 mg/kg; lethal dose) for 3 hours resulted in a moderate degeneration of hepatocytes with cytoplasmic vacuoles accompanied by extensive centrilobular necrosis, parenchymal hemorrhages, and large cytoplasmic vacuoles after 6 hours (Fig. 3A). Furthermore, we found a robust time-dependent increase in ALT that correlated with increases in circulating miR-122 after 3 hours and 6 hours of APAP treatment (Fig. 3B-D). Because APAP administration induces necrosis that could be accompanied by inflammation, we next examined the inflammatory miRNA, miR-155. We found a time-dependent increase in miR-155 in the plasma of mice treated with APAP (Fig. 3E) and other miRNAs involved in inflammation; miR-146a and miR-125b were also increased compared to saline controls (Fig. 3F, left and right). No changes were observed in plasma miR-181d expression after 3 hours of APAP treatment (data not shown). These results indicated that APAP administration results in a robust increase in hepatocyte specific miR-122 and a modest increase of inflammatory miRNAs (miR-155, -146a, and -125b) in the plasma and these miRNA signatures could potentially represent biomarkers of APAP-induced liver injury.
Increased Plasma MiRNA-122, -155, and -146a in Inflammation-Induced Liver Injury.
Emerging evidence suggests that both pathogen- and damage-associated molecular pattern molecules (PAMPs and DAMPs) contribute to liver inflammation. DAMPs include mitochondrial DNA and cells undergoing apoptosis that harbor CpG motif and is recognized by TLR9 and contribute to the activation of the downstream signaling cascade.16 Because of the emerging role of TLR9 in sterile inflammation, autoimmunity, and in liver diseases,16, 23, 24 we evaluated the efficacy of circulating miRNA as a marker of liver injury and inflammation after TLR9+TLR4 ligand administration.
Consistent with our previous report, CpG administration resulted in intense mononuclear inflammatory infiltrates in the liver and a further increase in inflammatory foci were observed when mice were challenged with LPS (Fig. 4A).24 Both plasma ALT and miR-122 were increased in LPS- or CpG-treated mice and a further augmentation in plasma ALT and miR-122 was observed in CpG-primed-LPS challenged mice (Fig. 4B,C). Moreover, a linear correlation existed between plasma ALT and miR-122 levels (Fig. 4D).
As there was increased infiltration of immune cells in CpG and in CpG-primed LPS-challenged mice, we next evaluated the level of inflammatory cytokine, TNF-α, and its positive regulator, miR-155. Both plasma miR-155 and hepatic TNF-α were increased in LPS- or CpG-treated mice with even higher levels in CpG-primed LPS-challenged mice (Fig. 4E, right and left). We found comparable levels of miR-155 both in plasma and serum fractions (Supporting Fig. 2). A linear correlation was observed between hepatic miR-155 and TNF-α levels (Supporting Fig. 3). We also found increases in plasma miR-146a in the presence of liver inflammation in the CpG model (Fig. 4F) but no change in miR-125b expression (data not shown). These observations suggested that plasma miR-122 and miR-155 mirror hepatocyte damage and inflammation, respectively.
Circulating MiRNAs Localize to the Exosome-Rich or Protein-Rich Compartments in a Liver Injury-Specific Manner.
Recent studies showed that extracellular miRNAs are associated with Ago complexes or packaged inside exosomes in cell lines or in healthy human plasma17, 25 but little is known about the fate of extracellular miRNAs after liver injury. Here, we examined exosome-rich or protein-rich (exosome free) fractions from the serum or plasma for miR-122 and miR-155 expression in the different liver injury models. We found that in alcohol-induced liver damage, which results in fat accumulation and increased inflammatory cytokines, both miR-122 and miR-155 were mostly associated with the exosome-rich fraction (Fig. 5A, left and right). A minimal increase in miR-122 and a decrease in miR-155 were observed in protein-rich fraction (Fig. 5A, left and right). Because of the induction of miR-122 and miR-155 in the circulation, we next tested total liver levels of these miRNAs and found that the hepatic expression of miR-122 was decreased in alcohol-fed mice, whereas miR-155 levels were moderately increased (Fig. 5B).
In APAP-induced liver injury associated with massive hepatocyte necrosis, miR-122 was more abundant in the plasma protein-rich fraction compared to the exosome-rich fraction (Fig. 5C, left). Moreover, a time-dependent increase in miR-155 was found in the protein-rich fraction with a concurrent decrease in exosome fraction (Fig. 5C, right). However, no significant changes were found in the hepatic miR-122 and miR-155 expression (Fig. 5D).
Finally, in the CpG+LPS-induced inflammation model that mainly causes inflammation, miR-122 and miR-155 were predominately increased in the exosome-rich fraction and decreased in the plasma protein-rich fraction (Fig. 5E, left and right). The hepatic expression of miR-122 was unchanged in LPS- or CpG-treated mice; however, CpG priming followed by LPS challenge resulted in its reduction (Fig. 5F). There was a robust induction of miR-155 by LPS and CpG treatment, which was further augmented after LPS challenge in CpG-primed mice (Fig. 5F).
To rule out the possibility that freeze/thaw interferes with miRNA levels in exosome- or protein-rich fractions, the expression of miR-122 and miR-155 were compared between fresh and frozen plasma samples after APAP treatment. The expression of these miRNAs in the protein- or exosome-rich fraction was comparable both in fresh and frozen samples (Supporting Fig. 4). The purity of the exosome fraction was confirmed by CD81 (exosome marker) expression and an increase in its level was observed only in the exosome-rich fraction (Supporting Fig. 5). Taken together, our results suggest that the distribution and extent of miR-122 and miR-155 (exosomes versus proteins) differs between ALD, APAP, and CpG-induced liver damage.
MicroRNAs have emerged as fine regulators of gene function and their presence in various body fluids identifies them as attractive potential biomarkers of disease.3, 5 In this report, we show that serum/plasma miR-122 and miR-155 may serve as biomarkers of liver damage and inflammation, respectively. Our results suggest that an increase in circulating miR-122 correlates with liver damage regardless of the etiology of hepatocyte injury. We also identified miR-155 as a candidate biomarker of liver inflammation. Our data demonstrate that miR-122 and miR-155 are present in the serum/plasma in exosome- or protein-rich fractions and show for the first time that miR-122 and miR-155 are associated with the exosome-rich fraction in alcoholic and inflammatory liver damage, whereas they are predominantly in the protein-rich fraction in APAP-induced liver necrosis.
Current clinical practice utilizes serum ALT/AST as markers of liver "injury"; however, these cannot distinguish between hepatocyte damage and inflammation. Serum cytokines are surrogate markers of liver inflammation but are not practical in routine clinical practice. Moreover, ALT and serum cytokines are not stable in extreme conditions and lack tissue specificity, creating a need for more sensitive, stable, and specific biomarkers of liver injury. Induction of miR-122 has been reported in HCC, HCV, and APAP-induced liver damage6-8 and our results further extend the feasibility of plasma/serum miR-122 as a marker of liver injury. Because our results indicated that increased serum/plasma miR-122 is universally associated with liver disease of different etiologies, the limitation of this potential biomarker is that it alone cannot distinguish between various etiologies of hepatocyte damage. Similarly, our results suggest that increased plasma/serum miR-155 cannot be used to distinguish among inflammation caused by various etiologies, i.e., alcohol uptake or DAMPs and/or drug overdose; however, the extent of its induction in the circulation varies between different etiologies. Future studies with broader miRNA profiling may reveal etiology-specific miRNA signatures in liver diseases.
Up-regulation of the inflammatory cascade has been attributed to Kupffer cell (KC) activation by gut-derived endotoxin in ALD.14 We recently reported that alcohol-induced miR-155 sensitizes KCs to LPS-induced TNF-α production in ALD.18 Here we found increased serum and liver miR-155 levels in alcohol-fed mice. Increased circulating miR-155 levels were also reported in rheumatoid arthritis and various cancer types26, 27 and our results support the increase of extracellular miR-155 during inflammation and its use as a marker of inflammation.27 Furthermore, we demonstrated that serum miR-122 increase in ALD was indeed a marker of liver disease rather than a direct effect of alcohol because mice deficient in TLR4 and p47phox subunit of NADPH oxidase enzyme complex (p47phoxKO) showed no increase in serum miR-122. Relevant to our finding, a recent study reported increased miR-122 in plasma after 6 hours of acute ethanol gavage in mice.8 To our knowledge our study is the first to demonstrate the induction of miR-122 in the circulation after prolonged alcohol feeding.
APAP-induced acute liver injury is a major cause of liver failure in Western countries.22 Here, we showed that plasma miR-122 that mirrored ALT levels could be exploited as a marker of APAP-induced liver injury. Our observation is consistent with a previous report; however, the dose and duration of APAP administration were different in our study.7 Similarly, a recent study demonstrated increased miR-122 in the plasma of APAP overdosed humans.28 However, in our study we found that not only the levels of plasma miR-122 but miR-155, miR-146a, and miR-125b were also increased after APAP treatment in a time-dependent manner. Our observation of increased miR-155, miR-146a, and miR-125b was somewhat unexpected because APAP injury is not associated with significant inflammation. Unlike in the alcohol-induced or inflammatory liver injury (CpG+LPS) model, where liver miR-155 levels were increased, we found no up-regulation of miR-155 expression in APAP liver injury, leading us to the speculation that the increase in plasma miR-155, miR-146a, and miR-125b could be a result of APAP-induced massive death of hepatocytes and immune cells in the liver rather than induction of the inflammatory miRNA repertoire. Further investigation of miRNA profile of APAP liver injury between lethal and sublethal doses may lead to more specific markers.
Emerging evidence suggests that endogenous danger signals (DAMPs) from dying cells, including DNA, induce sterile inflammation and autoimmunity.23 Endogenous danger signals such as mitochondrial DNA (TLR9 ligand) are likely to act on the liver in combination with gut-derived endotoxin (TLR4 ligand) present in various forms of liver diseases such as ALD, nonalcoholic steatohepatitis (NASH), and portal hypertension.19, 29, 30 In agreement with this, increased expressions of TLR9 and serum endotoxin have been reported in alcohol-fed mice.31, 18 Our finding of induction of miR-122 in the plasma after CpG treatment further extends the utility of circulating miR-122 as a marker of liver injury caused by DAMPs. Consistent with CpG- and/or LPS-induced inflammation, plasma levels of miR-155 were significantly increased. Induction of circulatory miR-155 has been reported in various cancers26, 27 and our finding indicates its induction in liver inflammation. Interestingly, miR-146a but not miR-125b was also increased in the plasma after CpG+LPS treatment. The finding of increased miR-146a in plasma after CpG+LPS treatment was surprising. In support of our data, increased levels of miR-146a have been reported in patients with type 2 diabetes idiopathic pulmonary hypertension and in patients with IgA nephropathy.32, 33 The significance of miR-146a induction in plasma in our study could be attributed to its role in limiting inflammation; however, further studies are warranted to rule out this possibility. Interestingly, only a modest increase in hepatic levels of miR-146a was observed after CpG treatment (Supporting Fig. 6), indicating its specific release into circulation. Although alcohol, APAP, and CpG all resulted in liver injury as indicated by ALT, the circulating miRNA signature and extent of miR-122 increases (Supporting Fig. 7) varied between the etiologies. To compare the characteristics of ALT versus miR-122 to discriminate between mice with liver injury (alcohol or APAP) and control mice, we calculated area under the receiving operating characteristics curve (AUROC). Although our analysis indicated that ALT and miR-122 have similar AUROC values in the ALD model, a slightly higher AUROC was found in the APAP model for miR-122 (Supporting Table 1). In the future, studies with large clinical sample sizes will be able to predict the superior sensitivity, diagnostics, and prognostics values of miR-122 over ALT.
Recent studies highlight the importance of extracellular miRNA in cell-to-cell communication and may also provide a mode of genetic information transfer.34, 35 Extracellular miRNA can be released from cells by way of different processes such as exosomes, microparticles, and proteins or unidentified mechanisms.36 The association of miR-122 with protein fractions has been demonstrated in healthy human plasma samples.17 However, a recent study suggests that a majority of miRNAs in the serum and saliva is concentrated in exosomes.37 Our results indicate that extracellular distribution of miRNA is distinct in different pathological conditions in the liver. In ALD and in inflammation (CpG/LPS)-induced liver damage both miR-122 and miR-155 increases were associated with an exosome-rich fraction. In contrast, miR-122 and miR-155 were predominantly increased in the protein-rich fractions in APAP/DILI, suggesting an injury-specific distribution of miRNA in the circulation. There could be multiple mechanisms by which miRNA enriches in exosomes versus protein fractions after different types of liver injury. For instance, in APAP-induced liver injury the damage is severe and rapid and miRNAs released primarily because of leakage from dying/damaged cells, which we speculate to explain why most miRNAs (miR-122 and -155) are found in the protein fraction (as miRNA normally bound in the RISC complexes within the cell). On the other hand, in more subtle injuries such as ethanol and CpG+LPS models, the cellular injury is slower and less severe and perhaps altered miRNA processing and release via exosomes, which could be the reason for enrichment in the exosome-rich fraction.
The tissue and cellular sources of the miR-122 and miR-155 increase are yet to be fully identified in liver disease. MiR-122 is abundant in hepatocytes, representing about 70% of the total miRNAs, and mechanisms underlying changes in liver expression of miR-122 are yet to be understood. MiRNA-155 is present but not limited to immune cells and our observation indicates that it is also present in hepatocytes.38 Based on the observation that liver miR-122 levels were decreased only in ALD and after CpG+LPS administration irrespective of its increase in circulation (ALD, APAP, and CpG+LPS), it is tempting to speculate that multiple hits may contribute to down-regulation of hepatic miR-122 levels, whereas damaged hepatocytes contribute to increased circulating miR-122 levels. However, further studies are required to discern the pathways responsible for decreased hepatic levels of miR-122 after liver injury. Contrary to our observation, Wang et al.7 reported decreased hepatic miR-122 levels after APAP treatment and this could be due to different APAP doses and timing.
In contrast to miR-122, miR-155 appears to be an inducible miRNA in alcoholic and inflammation (CpG+LPS)-induced liver disease, as its levels were increased not only in the serum/plasma but also in the liver. This is not unexpected, because TLR activation was shown to up-regulate miR-155 in immune cells and our study now shows that it can also be up-regulated in the liver. In immune cells and in KC, miR-155 induction involves nuclear factor kappa B (NF-κB) activation18 and NF-κB activation has been reported both in alcohol-induced liver disease and CpG+LPS-induced liver inflammation.18, 39 The lack of miR-155, miR-146a, and miR-125b increase in the liver along with an increase in the plasma after APAP intervention may be the result of cellular damage/death in the immune cell compartment, as APAP was shown to induce immune cell as well as hepatocyte death.15 Collectively, our results indicate stimuli-specific miRNA signatures both in the liver and in the plasma/serum. It will be interesting to explore what signal is required for the assembly of extracellular miRNA into exosomes versus protein compartments in liver injury.
- 1The functions of animal microRNAs. Nature 2004; 7006: 350-355..
- 2Emerging role of microRNAs in liver diseases. World J Gastroenterol 2009; 45: 5633-5640., , .
- 3Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res 2008; 10: 997-1006., , , , , , et al.
- 4Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 2008; 30: 10513-10518., , , , , , et al.
- 5Circulating microRNAs in hepatitis B virus-infected patients. J Viral Hepat 2011; 7: e242-51., , , , , .
- 6Circulating microRNAs, miR-21, miR-122, and miR-223, in patients with hepatocellular carcinoma or chronic hepatitis. Mol Carcinog 2011; 2: 136-142., , , , , , et al.
- 7Circulating microRNAs, potential biomarkers for drug-induced liver injury. Proc Natl Acad Sci USA 2009; 11: 4402-4407., , , , , , et al.
- 8Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical-related hepatic diseases. Clin Chem 2010; 12: 1830-1838., , , , , , et al.
- 9miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. Cell Metab 2006; 2: 87-98., , , , , , et al.
- 10MicroRNA-155 as a proinflammatory regulator in clinical and experimental arthritis. Proc Natl Acad Sci USA 2011; 27: 11193-11198., , , , , , et al.
- 11Mutator activity induced by microRNA-155 (miR-155) links inflammation and cancer. Proc Natl Acad Sci USA 2011; 12: 4908-4913., , , , , , et al.
- 12MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity 2010; 4: 607-619., , , , , , et al.
- 13MicroRNA in TLR signaling and endotoxin tolerance. Cell Mol Immunol 2011; 8: 388-403., , .
- 14The unfolding web of innate immune dysregulation in alcoholic liver injury. Alcohol Clin Exp Res 2011; 35: 782-786., , , .
- 15Acetaminophen hepatotoxicity and repair: the role of sterile inflammation and innate immunity. Liver Int 2012; 1: 8-20., , , .
- 16Type I interferons protect from Toll-like receptor 9-associated liver injury and regulate IL-1 receptor antagonist in mice. Gastroenterology 2011; 2: 697-708.e4., , , , .
- 17Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci USA 2011; 12: 5003-5008., , , , , , et al.
- 18Up-regulation of microRNA-155 in macrophages contributes to increased tumor necrosis factor {alpha} (TNF{alpha}) production via increased mRNA half-life in alcoholic liver disease. J Biol Chem 2011; 2: 1436-1444., , , , , , et al.
- 19The critical role of Toll-like receptor (TLR) 4 in alcoholic liver disease is independent of the common TLR adapter MyD88. HEPATOLOGY 2008; 4: 1224-1231., , , , , , et al.
- 20The presence of p47phox in liver parenchymal cells is a key mediator in the pathogenesis of alcoholic liver steatosis. Alcohol Clin Exp Res 2012 [Epub ahead of print]., , .
- 21NADPH oxidase-derived free radicals are key oxidants in alcohol-induced liver disease. J Clin Invest 2000; 7: 867-872., , , , , , et al.
- 22Review article: the current management of acute liver failure. Aliment Pharmacol Ther 2010; 3: 345-358., , , .
- 23Acetaminophen-induced hepatotoxicity in mice is dependent on Tlr9 and the Nalp3 inflammasome. J Clin Invest 2009; 2: 305-314., , , , , , et al.
- 24Critical role of toll-like receptors and the common TLR adaptor, MyD88, in induction of granulomas and liver injury. J Hepatol 2006; 6: 813-824., , , , , .
- 25Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line. PLoS One 2010; 10: e13247., , , , , , et al.
- 26Circulating microRNAs as blood-based markers for patients with primary and metastatic breast cancer. Breast Cancer Res 2010; 6: R90., , , , , .
- 27Plasma microRNAs as novel biomarkers for early detection of lung cancer. Int J Clin Exp Pathol 2011; 6: 575-586., , , , , , et al.
- 28Circulating microRNAs as potential markers of human drug-induced liver injury. HEPATOLOGY 2011; 5: 1767-1776., , , , , , et al.
- 29Toll-like receptor 9 promotes steatohepatitis by induction of interleukin-1beta in mice. Gastroenterology 2010; 1: 323-34.e7., , , , , , et al.
- 30The relationship between endotoxemia and hepatic endocannabinoids in cirrhotic rats with portal hypertension. J Hepatol 2011; 6: 1145-1153., , , , , , et al.
- 31Differential liver sensitization to Toll-like receptor pathways in mice with alcoholic fatty liver. HEPATOLOGY 2006; 5: 989-1000., , , , , , et al.
- 32Significance of serum microRNAs in pre-diabetes and newly diagnosed type 2 diabetes: a clinical study. Acta Diabetol 2011; 1: 61-69., , , , , , et al.
- 33Elevated levels of miR-146a and miR-155 in kidney biopsy and urine from patients with IgA nephropathy. Dis Markers 2011; 4: 171-179., , , , , .
- 34Secretory mechanisms and intercellular transfer of microRNAs in living cells. J Biol Chem 2010; 23: 17442-17452., , , , , .
- 35MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol 2011; 4: 423-433., , , , .
- 36
- 37The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PLoS One 2012; 3: e30679., , , .
- 38MicroRNA signature in alcoholic liver disease. Int J Hepatol 2012: 498232., .
- 39Diverse regulation of NF-kappaB and peroxisome proliferator-activated receptors in murine nonalcoholic fatty liver. HEPATOLOGY 2004; 2: 376-385., , , , , , et al.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
|Supporting Information
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|Supporting Information Figure 2.
|Supporting Information Figure 3.
|Supporting Information Figure 4.
|Supporting Information Figure 5.
|Supporting Information Figure 6.
|Supporting Information Figure 7.
|Supporting Information Table 1.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.


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iHerb Customer Reviews
good B12
2 people found the following review helpful
Posted by wendybrown on Nov 29, 2009
I like that this B12 comes from a food source and is in a capsule. Most B12's are not assimilated properly and this is a good one.
Vegan B12 cannot absorb in human body
9 people found the following review helpful
Posted by Reviewer1241148 on Mar 23, 2011
FYI, please do your research, Vit B12 MUST come from the animal source, otherwise it cannot be absorbed by a human body. I bought this product because usually I'm satisfied with GoL products, but didn't see that it's VEGAN:(((, too bad. Beware, make your research, you'll see that to test the amount of B12 in supplements they use bacteria that react to presence of b12, and they measure the reaction. Unfortunately our body cannot get any benefits from vegetarian source of b12.
Good B-12
1 person found the following review helpful
Posted by Reviewer1880085 on Jul 03, 2009
Love this product and the fact that it is raw.
Great for Vegetarians/Vegans
Posted by 5677816201077879284 on Nov 05, 2013
I love the energy boost I get from this. Will definitely buy again!
Vegetarian Must Have!
Posted by 5198507438601989301 on Dec 22, 2014
I have been neglecting supplements for a long time, but once I started taking this (along with vitamin D3, probiotics and magnesium) and couldn't believe what a difference it makes! Would recommend this!


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It's Official: ARC Tunnel Project Is Dead
New Jersey Gov. Chris Christie has cancelled plans for a new rail tunnel between New Jersey and Manhattan's Penn Station. The tunnel was supposed to more than double the number of commuter trains that could come into New York City and relieve many of the delays that passengers experience every morning.
"The current economic climate in New Jersey does not allow for this project to continue," the Republican governor says.
Christie put the project on hold last month to undertake a detailed cost review. On Thursday, he says that analysis showed that instead of costing $8.7 billion, the real cost will be closer to $12 to $15 billion.
Although the federal government would have paid for a large portion of the tunnel, New Jersey and the Port Authority would have been responsible for cost overruns. Christie says he's not letting New Jersey residents -- who he says are the most heavily taxed in the country -- take on that burden.
The Federal commitment to the project was capped at $3 billion. Christie says his administration will seek other ways to modernize and improve rail capacity.
The tunnel broke ground last summer, and so far, New Jersey has spent almost half a billion dollars on it. The state expects to get reimbursed for part of that from the Federal Transit Administration. The FTA wouldn't comment except to say that Transportation Secretary Ray LaHood expects to speak with Christie Firday to "discuss a path forward."
New Jersey residents have mixed feeling about the demise of a tunnel that could have seriously cut down their commute time.
John Adams says New Jersey needs the infrastructure:
"It's been postponed for too many years already, " Adams says. "I'd like to see it go through, albeit it's going to be expensive. We know that, but we need it."
Lydia Di Guido agrees with Christie that if the state doesn't have the money, then the tunnel should be a no-go:
"I wouldn't start an addition to my home if I couldn't finish it, so why would I want NJ state to do that," DiGuido says. "The way I run my home is the way we should run the government, If you can't afford it, don't do it."
Updated 3:10 p.m.


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Trustworthy reviews are our priority. Businesses can't pay to change or remove reviews.
Graydon Hall and its garden is absolutely beautiful and elegant and I can't really imagine holding our wedding and reception anywhere else. It was the first venue we visited and none of the other venues we visited afterwards could compare. There are certain aspects of the manor that may require some repairs but overall it is a gorgeous gem that is tucked in around apartment buildings.
We had a rough time with planning our wedding with Graydon Hall. From the time we signed the contract to the actual wedding, our event coordinator changed 3x over 14 months. It was pretty unsettling and people changing jobs can't be avoided but it definitely reaffirmed that we needed to hire our own wedding planner because we couldn't rely solely on the GHM coordinator to take care of everything. We also found the turnaround time for email responses were a bit slow and could sometimes takes weeks, and then response times were better closer to the date. GHM is also not very flexible in terms of negotiating anything.
The food is delicious and the portion sizes are HUGE. All of our guests were impressed by the meal and we were pleased that everything was exactly the same as our tasting. The wait staff is also incredibly attentive and made sure lunch was served quickly and smoothly.
While we had some hiccups during the planning process, the day itself was still executed perfectly and we couldn't be happier with how amazing our wedding day turned out.
Graydon Hall Manor was the PERFECT venue for our wedding. We chose to have a winter wedding and could not have chosen a better location (although it looks even more beautiful in the warmer months!). The building itself is gorgeous. The many rooms inside allow great use of the space. The food their caterer provided was phenomenal (and this was especially important to us as the groom is a Chef!). All in all, I do not think we could say one bad thing about this venue. Our guests were beyond thrilled and as were we!
Graydon hall is beautiful. I'm glad I picked this place for my wedding, service and food are excellent too.
Beautiful place, best food, excelent service. One wedding per day, so u don't have to share place with anybody else. Place is so beautiful that u can take all your pictures there and u don't have to go anywhere else.
Had our wedding at Graydon Hall Manor and we can't say enough good things about the place. We had ours in the 3rd week of May in 2013 and the weather was perfect. Not too warm and not cold at all. The sun was shining, not a cloud was in sight, and the garden was beautiful. Everything just looked perfect and all of our guests kept complimenting on how perfect our wedding was at Graydon Hall. The staff were exceptional as well, especially Keith, who coordinated with us throughout the months leading up to our wedding. He was very responsive, very helpful, very friendly, and we couldn't have asked for more!
Planning is a nightmare! The Organizer takes 1 week to respond and their excuse is that they are busy with many events that are earlier to my event.
However, the food and venue is a wonderful place! The service Captain and their team are wonderful and were very attentive with everything! If we need anything, they will drop what they are doing and will be to our rescue!
Although the planning stage was challenging, the day of the wedding was fabulous! Every guest enjoyed their time and even though it's long after the event, they still talk about how our wedding was amazing!
Our wedding coordinator at Graydon Hall, Keith Gawronski did an incredible job, along with all of the many staff. Keith kept us organized right up until the big day and on the big day the whole staff made things run smoothly and effortlessly. It poured with rain in the morning and they swept and cleaned and we were able to have the ceremony outside as a result. We received numerous compliments from our guests, as well as our band, on the food. While it is pricy, it was well worth it!


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Thaicom to Sell Mfone Business, Focus on Satellite
[Satellite TODAY 08-20-12]Satellite operator Thaicom will sell its Mfone mobile phone business to an operator outside of Thailand by the end of this year, according to the company's latest financial results issued Aug. 17.
Thaicom Spokesman Ken Streutker said the company is holding discussions with two prospective foreign operators about a possible takeover of the mobile firm, which will help the satellite service provider cut losses and bolster its finances.
"Mfone is the third largest mobile phone company in the Cambodian mobile market, with a 14 percent market share," Streutker said in a statement. "But, we expect to exit the Cambodian market through the sale of the company. We expect to maintain our revenue growth target of between 8 percent and 9 percent this year, driven by our satellite business."
Thaicom's 2012 second-quarter sales and service revenue both increased 3.4 percent from the same period last year. The operator's satellite revenues grew 17.5 percent year-on-year in the first half and contributed nearly 80 percent of Thaicom's total revenue.
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text: [ Former UM Law Professor Encourages States to Call For Article V Convention [WATCH] By Peter Christian March 25, 2015 Former University of Montana law professor, Rob Natelson, has released a video encouraging citizens throughout the country to consider an Article V convention of the states to amend the U.S. Constitution. Read More|Comment Category: Montana News, Newsletter Tags: Article V Convention, Balanced Budget Amendment, Independence Institute, Rob Natelson, U.S. Constutution Send to a friend! Share on Facebook Share on Twitter


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Rahm-a-drama: "Beet red" and "pissed" with the MSM
Awwwww. Rahm Emanuel can't stand the heat. So, he's blaming the media. ABC News says he's "beet red" and "pissed" that he can't go anywhere without someone asking question about his role in Blago-gate.
It's a hard-knock life:
The president-elect's chief of staff, Rahm Emanuel, said today he won't go to the Chicago presidential transition offices in order to avoid reporters trying to ask him whether he had contact with Illinois Gov. Rod Blagojevich about the Senate seat vacated by Barack Obama's election.
Emanuel appeared "beet-red," according to an ABC News cameraman who was invited inside by Emanuel to use his bathroom this morning.
"I'm getting regular death threats. You've put my home address on national television. I'm pissed at the networks. You've intruded too much, " Emanuel said, according to the cameraman.
A spokesman for Emanuel said he later showed up at the office, apparently able to sneak out of the house without being seen by reporters waiting to ask him questions.
Toughen up, sweetie. You're from Chicago. Remember?
Meantime, Blago's chief of staff resigned. But Blago's still holding on.
Tech firm gives 'cloud' backup of Hillary's (aka Val the bartender) email to FBI
October 6, 2015 08:53 PM by Doug Powers
According to Bill Clinton, the FBI is now part of the Vast Right Wing Conspiracy
September 27, 2015 08:47 AM by Doug Powers
Hillary lied about who approved Huma Abedin's job status change? NO WAY!
September 24, 2015 09:42 PM by Doug Powers
Shocker: FBI may have retrieved deleted emails containing classified info Hillary said were never on her server
September 23, 2015 08:52 AM by Doug Powers
Hillary's new selling point: I'm a Washington outsider
September 21, 2015 06:56 AM by Doug Powers
Categories: 2016 Campaign, Corruption, Democrats, Hillary Clinton


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HN-BV463 Bulova
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Bulova Concept Wall Clock - 9W x 12.75H in.
Be on top of all the info with one look at the Bulova Concept Wall Clock - 9W x 12.75H in.. The carbon-fiber patterned dial with contrasting white markers give a modern appeal. Included are a thermometer with Fahrenheit and Celsius scales and hygrometer.About Bulova CorporationThe realization of a great American dream began in 1875 when Joseph Bulova, a 23-year-old immigrant from Bohemia, opened a small jewelry store on Malden Lane in New York City. This modest enterprise was to evolve into one of


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Caffeine and Sodium Benzoate
Brain Pathway May Protect Against Cocaine Addiction »
"A study by researchers at the National Institutes of Health gives insight into changes in the reward circuitry of the brain that may provide resistance against cocaine addiction. Scientists found that strengthening signaling along a neural pathwa"...
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Caffeine and Sodium Benzoate Injection
Acute toxicity involving caffeine has been reported rarely. Mild delirium, insomnia, diuresis, dehydration, fever commonly occur with overdosage. More serious symptoms of overdosage include cardiac arrhythmias and clonic-tonic convulsions. In adults, IV doses of 57 mg/kg of body weight and oral doses of 18.50 grams have been fatal. In one 5-year-old patient, death occurred following oral ingestion of approximately 3 grams caffeine. Convulsions may be treated with IV administration of diazepam or a barbiturate such as pentobarbital sodium.
None known.
Last reviewed on RxList: 9/30/2008
This monograph has been modified to include the generic and brand name in many instances.
Caffeine and Sodium Benzoate Injection FDA Prescribing Information
Additional Caffeine and Sodium Benzoate Injection Information
Report Problems to the Food and Drug Administration
You are encouraged to report negative side effects of prescription drugs to the FDA. Visit the FDA MedWatch website or call 1-800-FDA-1088.
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Volkswagen Repair in Painter
Our master mechanics identify the best Volkswagen repair shops in Painter. Edit search×
There are 70 Volkswagen service shops and mechanics in Painter. Edit search
RepairPal has not certified the following shops.
The shops listed below are not committed to the fair price guarantee and minimum warranty of RepairPal Certified shops. When taking your car to a shop that has not passed RepairPal's inspection, we recommend that you:
- Look for a shop with ASE-certified technicians.
- Request written estimates from multiple shops and check them with the RepairPal estimator
- Verify that the shop you select is willing and able to clearly explain the work being performed.
Volkswagen repair shops and dealerships near Painter.
"This shop was professional and the wait time was also pretty much decent. I would return there with my vehicle for re..."
"Brady and his employees are honest and reasonable. Estimates (time and costs) are accurate. Have taken my car here f..."
"When Hurricane Irene was about two days away from hitting us in Hampton Roads, I noticed a fuel leak coming out of ou..."
"Good people--fair price"
- Audi
- Mercedes-Benz
- Porsche
- Volkswagen
These guys removed parts from my vehicle said that they were going to get replacement par..."


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I'm trying to find the Clerks 10th Anniversary DVD. I don't usually buy the dvd of a movie I've already seen, but I made a exception with Clerks. I'm also planning on buying Okami. I've been waiting for this game for a long time and after playing the demo, I was hooked. The visuals are excellent, the gameplay is tight and very good, and the story seems fresh. September 5th can not come fast enough...
The Saw 3 official site was recently revamped and now features wallpapers, icons, blood drive, and now the Trailer that was featured in The Descent. The trailer was good. Everyone now thinks Jigsaw is trying to play God now, thanks to the trailer... Note: He's not. It's just a attempt to "mystify" you. Saw 3 appears to be the final one in the series. Which is good. I don't want to see the movies turn into the next Jason or Halloween movies where there is at least 9 movies for both.
AS for My1up page, not much more will be added to it for a while. I'm going to be busy with the project I'm currently working on. Note: If you see a picture on it you liked and weren't able to save before they were removed, let me know and I'll try to retrieve it for you. Also, I'm going to do A "Top 5 list of flaws" for the PS3 and Wii. It's not just for the 360.


text: Staying at Villa Margarita was an all around great experience. Mike & Terry are wonderful hosts, they provided great tips and recommendations and met all our expectations. The Villa offers spacious, clean and very comfortable accommodations with great views and a beautiful pool. It is equipped with everything a traveler could possibly need for a wonderful vacation.


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updated 07/14/2011 AT 3:00 PM ET
•originally published 07/14/2011 AT 5:00 PM ET
Octuplets? Pfft! – that's for amateurs! Milly the dalmatian had twice as many babies when she gave birth to a litter of 16 puppies in May.
That number may be rare, but rarer still is the fact that Milly herself was born in a litter of 16 six years ago.


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Get Help
MVLP provides much-needed legal services to Mississippians of low-income and limited means through direct legal assistance and legal clinics throughout the year.
MVLP offers attorneys and students a wide range of volunteer opportunities. Most attorneys donate their time by providing advice at a legal clinic or pro bono representation to a MVLP client.
Each year, MVLP helps thousands of low-income Mississippians get access to civil justice. However, due to a lack of resources, too many low-income Mississippians still do not get the legal help they need.
2015 Pro Bono Awards Dinner
Thank you for joining MVLP's Second Annual "Achieving Justice, Restoring Hope" Pro Bono Awards Dinner held on September 10, 2015 at the Old Capitol Inn. MVLP greatly appreciates individuals, businesses, organizations and agencies who supported our 2015 fundraising efforts and helped us to recognize attorneys, law firms and law organizations throughout Mississippi who have been actively involved in delivering pro bono services to low-income individuals across Mississippi.


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MsJayeR Model
BRoe PhotographyPhotographer Male Suffolk, Virginia, US
My Website: FacebookMy MM URL: http://www.modelmayhem.com/RoePhotography
Mayhem # 2896995
About MeHey all, I've been shooting photography for the past 14yrs but in the past 2yrs I decided to take it a bit more serious. I'm currently a student at the New York Institute of Photography and I decided to join to see if this site would help me get models for some of my projects and future work. If you want to get a hold of me, this works well or you can stop by my Facebook page for "Roe Photography". Thanks for stopping by and I hope to work with some of you soon!!
MsJayeR Model
Saint June Model
Kayla Rivera Model
Rachael C Pepper Model
Alex Motteler Model
Heather Nicole Regan Model
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April 15, 2014 - Products
Bridgestone Commercial Solutions introduced a mobile app designed to help fleets and dealers quickly access and compare information about the Bridgestone family of commercial tire brands. Called Tire Advisor, the app is a one-stop resource connecting users to a comprehensive catalog offering multiple search capabilities with the ability to create a library for future reference.Responding to customer demands for mobile tools that provide detailed specifications on products, BCS created the app in an easy-to-use format tailored to the specific needs of commercial dealers and users of off-road and commercial truck tires as well as retread products.Additional Tire Advisor mobile app features include:
Tags: Information Technology, Bridgestone, Tires and Wheels, Mobile Apps, Tires
Go directly to this company's home page
We offer e-newsletters that deliver targeted news and information for the entire fleet industry.
The expert, Mark Betner from CITGO, will answer your questions
The expert, Rence Oliphant from Hendrickson, will answer your questions
LockLight Indicates When a Lift is Safe
Diesel Fuel Test Takes 20 Minutes
CT50 Mobile Computer Offers Flexible Features
Accuride App Displays Products and Information


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Paint Tester
By Luminant Software, Inc
Open iTunes to buy and download apps.
See how new colors would look on your walls before you paint!
Finding the right color for a room can be a challenge, but Paint Tester shows you the results without all the pain of painting and repainting! Easily try out colors from the palette or take a photo of a color you'd like to try, and apply it to pictures of your own home! Know what you're getting in to before the first drop of paint touches the walls.
Paint Tester's variety of tools and smart paint bucket make it easy to change the wall color in a picture in a matter of seconds!
Start by taking a picture of the room you'd like to paint, then use the paint bucket to apply colors to the walls. In a well lit photo, the smart paint bucket will color the wall without bleeding on to neighboring walls or the ceiling. This gives you the control to get a clean picture of how the color would look, and you can even use multiple colors at the same time to try an accent wall!
If you didn't like how the first color looks, use the color picker to choose another, and just tap with the paint bucket to replace it!
For great results try taping a color sample to the wall and take a photo of it. Use the color picker to select the sample, then use the paint bucket to start applying the color right next to the sample. Because the sample and the wall will be lit the same, this can take a lot of the guesswork out of how the color will look in the lighting of the room.
What's New in Version 3.0
1. The brush, eraser, and tape tools can now be dragged while zoomed in by turning on the scroll lock. 2. Updated UI 3. Stability improvements.
Customer Reviews
Works great!
This is a great app it you are thinking of changing the color of paint you have in a room. It is very simple to use and easy to fill an area with a new color to see what it would look like. This will give you a good idea of what a room might looks like before you shell out the bucks for the paint. Although it was a little tough to match the color exactly I believe this was a factor of the lighting and the camera it was easy to pick from the palette.
Fresh Start
We are just getting started on some decorating projects, so we have been trying some apps that would help us visualize without moving furniture/making hoes in the walls/testing colors with samples from the store. This is the first app that has been user friendly; simple to figure out and easy to undo. I have tried several design apps and have not been able to do anything with them except download them. Thanks for keeping it so simple!
In less than 5 minutes including downloading and taking the photo, I "painted" my foyer walls excepting the 2 levels of stairs, banisters, and rails. I taped off the general area with my finger, by selecting the blue tape roll, then filled three areas behind the stair rails by selecting the paint bucket icon and choosing my color. ALL IN LESS THAN 5 MINUTES I SAY!!!!! It automatically skipped banisters and rails included in the area!!!
- Free
- Category: Utilities
- Updated: Sep 17, 2014
- Version: 3.0
- Size: 5.6 MB
- Language: English
- Seller: Luminant Software, Inc
- © Luminant Software, Inc
Compatibility: Requires iOS 4.3 or later. Compatible with iPhone, iPad, and iPod touch.


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Our award winning range comprises over 1000 wines from every corner of the wine world. Boutique family wine growers sit side by side with exceptional co-operatives and other large scale producers who offer unparalleled value. You can search by country, grape variety, wine style, closure or of course producer name. If you want to search by wine name or product code, simply type it into the search box top right of your screen.
Pato Frio Antao Vaz 13 Ribafreixo 6/75cl(6x75cl)
Notes of tropical fruits overlaid with hints of citrus and minerals.
Douro Superior 13 Quinta do Crasto 6/75(6x75cl)
Colour: Deep violet.
Nose: Very expressive aromas, displaying vibrant berry fruit notes perfectly combined with subtle hints of fresh spices.
Palate: Charming on the palate. Berry fruit flavours, great volume, solid structure and velvety tannins. The finish is perfectly balanced, fresh and
Douro Red 14 Crasto 6/75(6x75cl)
Colour: Deep violet.
Nose: Fresh on the nose, with intense berry fruit aromas and elegant floral notes.
Palate: Balanced and well-structured on the palate, with berry fruit flavours. Tannins are round and finely-textured. Elegant and lingering finish.
Taylor's 10 YO Tawny 6/75(6x75cl)
An exceptionally fine old tawny blend, aged for an average of 10 years in oak casks. Elegant and smooth, combining delicate wood notes and rich mellow fruit, it is bottled for immediate drinking.
C da Passarella A Descoberta Red 11 6/75(6x75cl)
Great elegance and balance. Ripe red fruit.
Gaudio Classico 6/75 Ribafreixo 12(6x75cl)
Blackberries in jam, spices and toast
Abanico Red Res. 11 Casa Passarella 6/75(6x75cl)
Full bodied, ripe red fruit with notes of cocoa and sweet spices.
Douro White 13 Crasto 6/75(6x75cl)
Bright and pale lemon yellow in the glass with an enticing minerality on the nose followed up with crunchy green apple, camomile and lemons. Fresh attack on the palate leading into a fresh and crisp finish.
Douro White 14 Crasto 6/75(6x75cl)
Bright and pale lemon yellow in the glass with an enticing minerality on the nose followed up with crunchy green apple, camomile and lemons. Fresh attack on the palate leading into a fresh and crisp finish.
Vinha Maria Teresa 11 Q do Crasto 3/75(3x75cl)
This very special wine is only made in outstanding vintages and incorporates up to 30 different grape varieties in the final blend. The wine has an intense, vibrant violet colour which leads to a deep, complex nose full of wild forest flowers, white pepper and soft dark chocolate aromas. It is a full bodied, richly flavoured wine with superb tannins, refreshing acidity and concentrated fruit.


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Nubian Skin: Lingerie Line For Women of Color
Nude undergarments are a must-have for women who want a polished look when wearing sheer or white clothing. But, for many women whose skin tones are darker than the average version of "nude" available on the market, which is usually a shade of beige, finding the perfect seamless undergarment can be difficult. A new hosiery and lingerie line, NubianSkin, is aiming to change that with the introduction of a line of essential undergarment needs, available in suitable skin-tones for women of color.
Nubian Skin founder, Ade Hassan, developed the line after being frustrated by the lack of undergarments that met the needs of ethic women. With the new line, women can choose from four different dark skin tone shades including Café au Lait, Caramel, Cinnamon and Berry. The company has a skin-tone matching guide on their website to help customers select the hue that best suits them.
Nubian Skin, which is based in London, recently launched at Nordstrom stores in the US and ships worldwide. Learn more.
Find Your Favorite Florals In Essence of Beauty Products!
Like many of you, the thing I love the most about summer is all of the beautiful flowers blooming and fragrance the air. Skincare and fragrance line, Essence of Beauty, evokes summer in a bottle with their products which features in-season florals, so you can experience the floral aroma all year long.
Essence of Beauty Luscious Peony Fine Fragrance Body Mist and Body & Hand Cream – Evoking a lavish floral garden bursting with freshness, white grapefruit, peony and magnolia exude a vibrant spirit that exhilarates.
Fine Fragrance Body Mist: $6.99
Body & Hand Cream: $6.99
Essence of Beauty Rose Enchantment Fine Fragrance Body Mist
Escape to a vibrant and lush rose garden with top notes of bergamot and radiant orange, mid notes of strawberry, elegant rose and geranium bloom, and base notes of garden rhubarb, sunshine daisy and dewy musk.
Fine Fragrance Body Mist: $6.99
Essence of Beauty Body Oil in Lavender with Rosemary Oil
Highly restorative blend of exotic oils rich in antioxidants, vitamins and essential nutrients balances and nourishes skin, absorbing quickly for all-day hydration.
Nourishing Body Oil: $9.99
Essence of Beauty exclusively sold at CVS/pharmacy stores nationwide.
Summer Jewelry Must-Have: Efva Attling
Post submitted by Jolevette
With warmer temperatures now here, I have been shedding my winter coat for lighter styles! And what is better to add to your summer ensemble than some flash? I visited Efva Attling for summer inspired pieces, and this designer did not disappoint.
From Sweden and a model, Ms. Attling has been designing jewelry since the age of eleven. But the runway could not stop her from her pursuing her passion – jewelry design. Ms. Attling's collections each tell a story. Working with precious metals and precious stones, she created strings of butterflies to a starry sky at night. Wearing one of these pieces will certainly make you stand out from the crowd.
One of my favorite pieces in the store was a ring made into a cross made of 18 carat gold, and conflict free diamonds with the highest clarity.
And for the man looking to pop the question, Ms. Atling has a wedding selection that would make any future bride scream "YES!" The mixture of colorful precious stones and diamonds are sure to turn heads and make her feel all the more special because of the unique designs.
So, whether you are looking for that once of a kind piece, or to let someone know that they are the one, Efva Attling's designs is for you. Stop in and indulge yourself or your love.
Location in NYC:
36 Little West 12th Street,
New York, NY 10014
Fashion that Offers Sun Protection
It's important to protect your skin from potential sun damage all year-long, and one of the best ways to do that is by wearing sunscreen. But, if you want an added layer of protection, while still looking chic, check out some of the accessories from Bloxsun. The company offers what they call "undercover sunwear" with UPF (Ultraviolet Protection Factor) 50+ fabric proven to block 98% of the sun's UV rays without the use of chemicals or additives.
The thin, delicate skin on the hands is more prone to premature aging than all other parts of the body. When driving or participating in outdoor activities, you can wear Bloxsun Sun Gloves ($40, bloxsun.com), which offer discreet yet effective sun protection. The nude-colored stretch nylon gloves feature friction dots on the palms to provide UV protection without compromising grip.
And, while running around town or lounging at the pool, you can wear Bloxsun Sun Scarves ($68, bloxsun.com) to reduce the risk of sun damage and skin cancer. Wear it over your head or as a wrap.
For more information, click here.
Hot New Beauty Products to Try This Spring
Last week I attended the 10th Anniversary arty of New Beauty magazine where a variety of different beauty companies were on hand to celebrate the magazine, as well as show off their products to the room filled with beauty experts. Some of the items on display were products that we all may be familiar with, while others are newcomers eager to make their presence known on the beauty scene. Here are a few my top picks for products to watch out for this year.
Real Techniques Bold Metallics Collection – Developed by Youtube sensation Pixiwoo's Sam and Nic, this new collection of brushes were inspired by luxury. The soft bristles featured tapered tips and are engineered for perfect weight distribution when tipped toward the face while being used. The brushes are available at ulta.com and boots.com.
BeautyDNA – This new subscription box service will deliver a full-sized product to your door every moth customized to your skin needs. Subscribers fill out a detailed Beauty Profile, the system makes a product match, and then the product is shipped directly to you with a detailed report explaining why it was selected specifically for you.
SuperSmile – This professional at-home teeth whitening line has been around since 1987. The system removes tooth stains without bleaches or abrasives, when users follow the 6-minute regimen developed by the company that includes Activating Rods applied to the teeth, brushing with the Whitening Toothpaste and rinsing with its Whitening Pre-Rinse.
Intellishade – This anti-aging tinted moisturizer is available in Original Radiant and a new matte formula and features spf 45 and features one shade that blends in with every skin tone. It's a multi-tasking product, perfect for women on the go as it is a sunscreen, moisturizer, makeup and ant-aging product in one.
Get The Look: Jennifer Hudson's Black Jumpsuit
Jennifer Hudson stunned on the red carpet on March 28th wearing a fabulous black jumpsuit to the 28th annual Nickelodeon Kids' Choice Awards in Inglewood, California. The all-black fitted number stood on its own with a sexy v-neckline, while the only bright pops of color were seen with the purple lipstick she rocked and the blue strap on the sassy sandals that adorned her feet. Perfect for Spring, here are some black rompers you can wear, inspired by the American Idol starlet.
Product Review: Estée Lauder Double Wear Stay-In-Place Eye Pencil
If you've viewed my Instagram page lately (@delia_licious) you saw a quick snapshot I did using colors from Estée Lauder's new Double Wear Stay-In-Place Eye Pencil collection.
Available in 16 gorgeous shades, the 12-hour wear eye pencils are not just long-lasting, but waterproof, so you can define your eyes and not have to work about the product disappearing or smudging. I wear the colors from day-to-night and found that they are in fact long-wearing and do not fade at all. I have the teal, gold and sapphire blue, and the colors are bright and vibrant; moreover, they really do apply true to the way they look on the outside of the pencil.
When I applied the product, it glided on smoothly and effortlessly. I also liked that the pencil features a smudger on the other end for to facilitate creating a smokey eye look. I actually used the smudger to create the winged eyeliner look seen in the picture above.
The eye pencils retail for about $23.
Black Friday – Cyber Monday Beauty Deals
For November 28th only, all MAKE UP FOR EVER boutiques will be offering the following promotions on top products from the brand:
• MAKE UP FOR EVER Aqua Shadow: 2 for $10- all colors
• MAKE UP FOR EVER Aqua Eyes Lagoon Green: 2 for $10
• MAKE UP FOR EVER Aqua Liner, Sepia Collection: 2 for $10
• MAKE UP FOR EVER Make Up Artist Picks Set: was $300, new price $150
• MAKE UP FOR EVER Artisan Brush Set: was $65, new price $34
• MAKE UP FOR EVER Aqua Shadow Kits: was $38, new price $19
• MAKE UP FOR EVER Extravagant Eyes on the Go Set: was $18, new price $9
Additionally, on Black Friday MAKE UP FOR EVER will be launching the "Get Glossy" kit exclusively in Sephora stores, featuring Lab Shine Lip Gloss in shades #D14 & #D16 for $10 (the glosses retail separately for $19 each).
Black Friday – Cyber Monday
• From November 28 – December 2 ghd will offer 25% off all of ghd's innovative hair tools (excluding the ghd curve collection) on ghdhair.com.
• ghd will also offer ghd gift cards starting on November 21
Red Door Spa
Black Friday
• Get 15% off retail purchases of $100 or more on RedDoorSpas.com
• Get a complimentary upgrade to a Signature Service with the purchase of an Essential Facial or Swedish Massage
Iluminage Beauty
Black Friday – Cyber Monday
• From November 28 – December 1, buy two pillowcases for $99 with free shipping on IluminageBeauty.com with promo code beautysleep
New Site Let's You Try Samples From Leading Brands for Free
Last week I attended the one-year anniversary of PINCHme, a sampling service that allows users to choose and review products of their choice for free. The event also marked the company's one-million user benchmark, proving that this start-up has a strong following behind its innovative concept of empowering consumers with the ability to discover and discuss products ranging from beauty to food, without any cost. Below is how the program works:
Products are released for a limited time, and after your receive your product, all you have to do is log on to your account and provide your feedback. Click here to sign up or to learn more.


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Social connectedness improves health outcomes and quality of life, and saves lives and lowers system-wide costs. Socially-connected people in general live 3-10X longer than those that are not!
Private, Mobile, Social, Patient Networks
We are a matchmaking and engagement platform for patients. We help patients find each other, and give them private, mobile networks that deliver the connections, resources and guidance needed for successful health management.
Curatio is a mobile-first platform. We believe in putting the power of health in the palm of your hand. Curatio networks are with you wherever you go, whenever you need them.
Curatio circles are private and secured networks comprised of other patients, practitioners, caregivers, family and friends that you choose. Our intelligent matchmaking platform helps you find patients just like you.
We make it easy for you to find trusted support and information tailored to your needs. Our networks are easy to navigate and filled with validated resources that give you the knowledge and connections you need.
Health organizations
Extend virtual support groups to widespread populations
Hospitals and Clinics
Mobilize care plans and post-release support
Increase patient engagement and self-management outcomes
Improve drug knowledge and adherence strategies
Heart and Stroke
Healing Circles
Cardiovascular disease is the number one killer of women worldwide. Prevention and support are key but it's often hard to reach busy women through traditional programs. Heart and Stroke Foundation of Canada is leveraging Curatio to connect women post-diagnosis for support, healing and empowerment.
Young adult cancer survivors often find that their social ties are fractured and friends have moved on. They face unique challenges that greatly benefit from peer support. Will2Thrive uses Curatio to connect cancer warriors around the world and provide the community and resources to help them thrive!
★ Winner of Novartis Health 2.0 Global Competition
Thalassemia, an inherited blood disorder, affects 5% of the world's population. Managing this disease is complex and can be very isolating. Thalassemia International Federation has partnered with Curatio to use our social health solution for millions of people around the world.
Selected as 1 of 5 Canadian companies for CTA Silicon Valley
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