text:
- Ghosts in the Machines (Courtney Nash) — People are neither masters of machines, nor subservient to their machine-learning outcomes — we cannot, and should not, separate the two. We are actors, together, in a very complex system. David Woods calls this "joint cognitive systems."
- TLA+ (Leslie Lamport) — two tutorials: "Principles of Concurrent Computing" and "Specification of Concurrent Systems." Ironically, I see people grizzling that the book on distributed systems hasn't been linearised. I wonder if you can partition it into the two tutorials and still have full availability…
- Deep Learning vs Probabilistic vs Logic — As of 2015, I pity the fool who prefers Modus Ponens over Gradient Descent.
- Touché (Disney Research) — measur[es] capacitive response of object and human at multiple frequencies, a technique that we called Swept Frequency Capacitive Sensing. The signal travels through different paths depending on its frequency, capturing the posture of human hand and body as well as other properties of the context. The resulted data is classified using machine learning algorithms to identify gestures that are then used to trigger desired responses of the user interface.
"ai" entries
Four short links: 14 May 2015
Human-Machine Cooperation, Concurrent Systems Books, AI Future, and Gesture UI
Creative computing with Clojure
Exploring Clojure as a tool to generate music, visual art, poetry, and dance.
Clojure is gaining traction and popularity as a programming language. Both enterprises and startups are adopting this functional language because of the simplicity, elegance, and power that it brings to their business. The language originated on the JVM, but has since spread to run on the CLR and Node.js, including web browsers and mobile devices. With this spread of practical innovation, there has been another delightful development: a groundswell of people making art with Clojure.
Getting creative with Clojure
Creative Computing combines the power and engineering of the computer with the artistic inspirations of humans. People are using Clojure as a tool to generate music, visual art, poetry, and even dance. This ability to harness technology for creative purposes is both exciting and important. For it not only touches the heart and inspires existing technologists, but it also transcends all barriers. Art is a gateway to bring new people, young and old, from all walks of life, to the field of programming.
Let's explore some of the areas of Creative Computing with Clojure, and showcase some inspiring examples from a selection of artist/programmers. We'll look at projects that touch on music, art, games, writing, and even robots.
Four short links: 6 May 2015
Self-Driving Cars, Cloud BigTable, Define "Uptime," and Continuous Delivery Architectures
- Andrew Ng (Wired) — I think self-driving cars are a little further out than most people think. There's a debate about which one of two universes we're in. In the first universe it's an incremental path to self-driving cars, meaning you have cruise control, adaptive cruise control, then self-driving cars only on the highways, and you keep adding stuff until 20 years from now you have a self-driving car. In universe two you have one organization, maybe Carnegie Mellon or Google, that invents a self-driving car and bam! You have self-driving cars. It wasn't available Tuesday but it's on sale on Wednesday. I'm in universe one. I think there's a lot of confusion about how easy it is to do self-driving cars. There's a big difference between being able to drive a thousand miles, versus being able to drive anywhere. And it turns out that machine-learning technology is good at pushing performance from 90 to 99 percent accuracy. But it's challenging to get to four nines (99.99 percent). I'll give you this: we're firmly on our way to being safer than a drunk driver.
- Google Cloud BigTable — Google's BigTable, with Apache HBase API, single-digit millisecond latency, and "fully managed". G are hell-bent on catching up with Amazon and Microsoft at this cloud serving thing.
- Call Me Maybe: Aerospike — We're setting a timeout of 500ms here, and operations still time out every time a partition between nodes occurs. In these tests we aren't interfering with client-server traffic at all. Aerospike may claim "100% uptime", but this is only meaningful with respect to particular latency bounds. Given Aerospike claims millisecond-scale latencies, you may want to reconsider whether you consider this "uptime".
- 31 Continuous Delivery Architectures (Slideshare) — from a vendor, so one name crops up repeatedly (other than "Jenkins"), but it's still good devops voyeurism/envy.
Four short links: 16 April 2015
Relationships and Inference, Mother of All Demos, Kafka at Scale, and Real World Hardware
- DeepDive — DeepDive is targeted to help users extract relations between entities from data and make inferences about facts involving the entities. DeepDive can process structured, unstructured, clean, or noisy data and outputs the results into a database.
- From the Vault: Watching (and re-watching) "The Mother of All Demos" — "I wish there was more about the social vision for computing—I worked with him for a long time, and Doug was always thinking 'how can we collectively collaborate,' like a sort of rock band."
- Running Kafka at Scale (LinkedIn Engineering) — This tiered infrastructure solves many problems, but it greatly complicates monitoring Kafka and assuring its health. While a single Kafka cluster, when running normally, will not lose messages, the introduction of additional tiers, along with additional components such as mirror makers, creates myriad points of failure where messages can disappear. In addition to monitoring the Kafka clusters and their health, we needed to create a means to assure that all messages produced are present in each of the tiers, and make it to the critical consumers of that data.
- 3D Printing Titanium, and the Bin of Broken Dreams — you will learn HUGE amounts on the challenges of real-world manufacturing by reading this.
Four short links: 30 March 2015
Philosophical Research, Reading Turing, Security Exercises, and Golang Madness
- The Trolley and the Psychopath — Not only does a "utilitarian" response ("just kill the fat guy") not actually reflect a utilitarian outlook, it may actually be driven by broad antisocial tendencies, such as lowered empathy and a reduced aversion to causing someone harm. Questionably expanding scope of claims in the behavioural philosophy research. (via Ed Yong)
- Summary of Computing Machinery and Intelligence (1950) by Alan Turing (Jack Hoy) — still interesting and relevant today. cf Why Aren't We Reading Turing
- Exploit Exercises — a variety of virtual machines, documentation, and challenges that can be used to learn about a variety of computer security issues, such as privilege escalation, vulnerability analysis, exploit development, debugging, reverse engineering, and general cyber security issues.
Four short links: 18 March 2015
Moonshots, Decacorns, Leadership, and Deep Learning
- How to Make Moonshots (Astro Teller) — Expecting a person to be a reliable backup for the [self-driving car] system was a fallacy. Once people trust the system, they trust it. Our success was itself a failure. We came quickly to the conclusion that we needed to make it clear to ourselves that the human was not a reliable backup — the car had to always be able to handle the situation. And the best way to make that clear was to design a car with no steering wheel — a car that could drive itself all of the time, from point A to point B, at the push of a button.
- Billion-Dollar Math (Bloomberg) — There's a new buzzword, "decacorn," for those over $10 billion, which includes Airbnb, Dropbox, Pinterest, Snapchat, and Uber. It's a made-up word based on a creature that doesn't exist. "If you wake up in a room full of unicorns, you are dreaming," Todd Dagres, a founding partner at Spark Capital, recently told Bloomberg News. Not just cute seeing our industry explained to the unwashed, but it's the first time I'd seen decacorn. (The weather's just dandy in my cave, thanks for asking).
- What Impactful Engineering Leadership Looks Like — aside from the ugliness of "impactful," notable for good advice. "When engineering management is done right, you're focusing on three big things," she says. "You're directly supporting the people on your team; you're managing execution and coordination across teams; and you're stepping back to observe and evolve the broader organization and its processes as it grows."
- cxxnet — "a fast, concise, distributed deep learning framework" that scales beyond a single GPU.
Four short links: 17 March 2015
Open Source Personal Assistant, Flintstoning Robots, Year of Personal Assistants, and Infrastructure Curiosity
- Sirius — UMich open source "intelligent Personal Assistant" (aka Siri, Cortana, Google Now, etc.). Text recognition, image recognition, query processing components. They hope it'll be a focal point for research in the area, the way that open source operating systems have focused university research.
- MIT DragonBot Evolving to Teach Kids (IEEE Spectrum) — they're moving from "Wizard of Oz" (humans-behind-the-scenes) control to autonomous operation. Lovely example of Flintstoning in a robotics context.
- Personal Assistants Coming (Robohub) — 2015 is the year physical products will be coming to market and available for experimentation and testing. Pepper ships in the summer in Japan, JIBO ships preorders in Q3, as does Cubic in the fall and EmoSpark in the summer. […]The key to the outcome of this race is whether a general purpose AI will be able to steer people through their digital world, or whether users would rather navigate to applications that are specialists (such as American Airlines or Dominos Pizza).
- Incuriosity Killed the Infrastructure — being actively curious about "fishy" things will lead to a more stable and happy infrastructure.
Four short links: 16 March 2015
Turing and Bechdel, Women in Leadership, Coding Messaging, and VR Affordance
- Ex Machine: When Turing Meets the Bechdel Test — The Bechdel Test is useful for more than examining gender representation. It can be our Turing Test for creating believable alien or artificial life forms. If you look at our filmic or cultural representations of the other or alien, then you have to be struck by the singular nature of them all. From Frankenstein to Big Hero 6, do they have any reality without the central human characters? No, they are alone. Even Alien is alone. At least in Frankenstein, it is the utter aloneness of the new form that is the whole story. Films that have pushed the envelope are few. And doing a quick mental check, one was left feeling empathy for the 'others' in only a couple, like Westworld, BladeRunner, and Planet of the Apes, and the books of writers like Brin and Cherryh.
- Women in Business: The Path to Leadership (PDF) — Grant Thornton International Business Report on women in senior roles, operational and governance. Ends with some sound recommendations (via Rowan Simpson).
- Adventures in Messaging Queues — extremely readable tale of developing a custom piece of distributed infrastructure.
- Valve's SteamVR — What these two experiences shared was an overpowering sense of intimacy with the virtual space. This is brought home again and again in the demos, and its impact is profound. The smallness of the space you can move around – 15 feet square – is almost a virtue in this respect, because it focuses you on your immediate surroundings in a way the vast majority of video games don't, and which has much more in common with real life.
Four short links: 5 March 2015
Web Grain, Cognition and Computation, New Smart Watch, and Assessing Accuracy
- The Web's Grain (Frank Chimero) — What would happen if we stopped treating the web like a blank canvas to paint on, and instead like a material to build with?
- Bruce Sterling on Convergence of Humans and Machines — I like to use the terms "cognition" and "computation". Cognition is something that happens in brains, physical, biological brains. Computation is a thing that happens with software strings on electronic tracks that are inscribed out of silicon and put on fibre board. They are not the same thing, and saying that makes the same mistake as in earlier times, when people said that human thought was like a steam engine.
- Smart Pocket Watch — I love to see people trying different design experiences. This is beautiful. And built on Firefox OS!
- Knowledge-Based Trust (PDF) — Google research paper on how to assess factual accuracy of web page content. It was bad enough when Google incentivised people to make content-free pages. Next there'll be a reward for scamming bogus facts into Google's facts database.


text:
Enanta Pharmaceuticals to Present at the 39th Annual Deutsche Bank Securities Healthcare Conference Presentation to be Webcast on May 7, 2014 at 4:50 P.M. ET Deutsche Bank 39th Annual Health Care Conference Business Wire WATERTOWN, Mass. -- April 30, 2014 Enanta Pharmaceuticals, Inc., (NASDAQ:ENTA) a research and development-focused biotechnology company dedicated to creating small molecule drugs in the infectious disease field, today announced that Jay R. Luly, Ph.D., President and Chief Executive Officer, will present on Wednesday, May 7, 2014, at 4:50 p.m. ET during the 39^th Annual Deutsche Bank Securities Healthcare Conference in Boston. The presentation format will be conducted as a fireside chat, or interview format and will be webcast live. A live webcast and replay of the presentation can be accessed by visiting the "Calendar of Events" section on the "Investors" page of Enanta's website at www.enanta.com. A replay of the webcast will be available following the presentation and will be archived for approximately 30 days. About Enanta Enanta Pharmaceuticals is a research and development-focused biotechnology company that uses its robust chemistry-driven approach and drug discovery capabilities to create small molecule drugs in the infectious disease field. Enanta is discovering, and in some cases developing, novel inhibitors designed for use against the hepatitis C virus (HCV). These inhibitors include members of the direct acting antiviral (DAA) inhibitor classes – protease (partnered with AbbVie), NS5A (partnered with Novartis) and nucleotide polymerase – as well as a host-targeted antiviral (HTA) inhibitor class targeted against cyclophilin. Additionally, Enanta has created a new class of antibiotics, called Bicyclolides, for the treatment of multi-drug resistant bacteria, with a focus on developing an intravenous and oral treatment for hospital and community MRSA (methicillin-resistant Staphylococcus aureus) infections. Contact: Investor Contact Enanta Pharmaceuticals, Inc. Carol Miceli, 617-607-0710 firstname.lastname@example.com
Enanta Pharmaceuticals to Present at the 39th Annual Deutsche Bank Securities Healthcare Conference
Press spacebar to pause and continue. Press esc to stop.

subset: commoncrawl


text:
An oversized item surcharge may apply to some heavy or bulky items due to size, weight or special handling required. This item surcharge is in addition to standard shipping rates and not included in shipping promotions.
O'Neill is one of the leaders in beach lifestyle sportswear. These sandals feature adjustable ankle straps, memory foam padding, braid detailing and rubber outsole for traction.
Web Id: P000311331


text:
1950-A dedication of the monument erected in Arlington National Cemetery on the gravesite of those who lost their lives on the night of 29 January 1945, when USS Serpens was destroyed off Lunga Beach, Guadalcanal. This was the largest single disaster suffered by the Coast Guard in World War II.
1992: The CGC Storis became the cutter with the longest service in the Bering Sea, eclipsing the U.S. Revenue Cutter Bear which had held that distinction since 1929. The Bear was decommissioned in 1929 after serving in the Bering Sea for 44 years and two months.
(Source: USCG Historian's Office)
Greece said on Tuesday it had no plan to carry out joint sea patrols with neighbouring Turkey to stem an influx of migrants and refugees into Europe.
A record 400,
The global salvage business continues to be very dynamic with emerging trends and significant moves made by major salvors. While not as heavily affected by primary
HII president and CEO Mike Petters honored at the Coast Guard Foundation's Salute to the United States Coast Guard in New York
Mike Petters, president and CEO
The Shipping Corporation of India Ltd., has won the third prize for the 'Best Enterprise Award' in the 26th Regional Meet organized by the Forum of Women in Public Sector (WIPS), Western Region.
Norwegian Energy Company ASA (Noreco) announces that Tommy Sundt is stepping down as CEO of Noreco ASA.
Since joining Noreco in September 2014, Tommy Sundt
Global marine terminal operator DP World has collected an impressive four awards including the Grand Prix of 'Leading Corporate for Investor Relations in the


text:
How Fast Passes Are Ruining Our Nation, Hyperbole… or Not?
SeaWorld Releases Four Rescued Manatees
Asaka, Braille, Nitty and Pipsqueak, four female sub-adult manatees, were happily released into the warm waters of Eddy Creek on Thursday July 18, 2013 by SeaWorld Orlando's Rescue Team. Three of these manatees were rescued in December of 2012 after being trapped in a mosquito embankment in the Merritt Island…
Theme Park Wars Forgotten Chapter: The Marvel Universe
SeaWorld Parks & Entertainment CEO Jim Atchison Profiled
Global Themepark Attendance For 2012 Via TEA Report
Univeral Studios Hollywood Will Go Forward With Expansion, Harry Potter
I spent most of the 90s visiting Universal Studios Hollywood almost every month. In doing so, I really got to know the tightly packed acreage that made up Southern California's second most popular themepark. It's taken some doing, but they've finally gotten final approval to go forward with the next…
Beyond the World's Fair


text:
canvas prints, fusion photography canvas prints, jeff burgess canvas prints, imagesfx canvas prints, abstract reality canvas prints, textured abstract canvas prints, textured landscape canvas prints, landscape canvas prints, moss canvas prints, flowers canvas prints, iris canvas prints
"Another Mythical Landscape" by Jeff Burgess printed on premium canvas and stretched on 1.5" x 1.5" stretcher bars (gallery wrap) or 5/8" x 5/8" stretcher bars (museum wrap). Ships within 3 - 4 business days and arrives ready-to-hang with pre-attached hanging wire, mounting hooks, and nails. Choose from multiple print sizes, border colors, canvas materials, and frames.
Corner Detail: Stretched canvas print with 1.5" stretcher bars and mirrored image sides. Also available with black sides, whites sides, and 5/8" stretcher bars.
Bring your artwork to life with the texture and depth of a stretched canvas print. Your image gets printed on one of our premium canvases and then stretched on a wooden frame of 1.5" x 1.5" stretcher bars (gallery wrap) or 5/8" x 5/8" stretcher bars (museum wrap). All stretched canvases ship within 3 - 4 business days and arrive "ready to hang" with pre-attached hanging wire, mounting hooks, and nails.
Stretched canvas prints look beautiful with or without frames.
Fine Art America is one of the largest, most-respected giclee printing companies in the world with over 40 years of experience producing museum-quality prints. All of our prints are produced on state-of-the-art, professional-grade Epson printers. We use acid-free papers and canvases with archival inks to guarantee that your prints last a lifetime without fading or loss of color.
Fusion photography. Another in a series of mythical landscapes. Nature is incredibly complex and dynamic on a biologic and metaphysical level. I wanted to portray this complexity through this fused medium. All photographic elements are my own. The image has been extensively manipulated via multiple filters and other strategies. The background image is of Mount Ranier, or as the native population of Washington State calls it "Mount Tahoma". Flowers are found in Washington State and the Pacific Northwest. All of them were imaged in my neighborhood and the beautiful gardens surrounding Revenna. The arch is in the backyard of a home that is otherwise in neglect. I don't think they have any idea of this most beautiful object that is...
This is the official version: Jeff Burgess is a fine-art photographer specializing in 'fusion' work involving the application of multiple layers of images to create the final vision. His work has been in numerous galleries including the Dunville and Warren Knapp in Seattle, Gallery 8 in Phoenix, The Center for Fine Art Photography in Denver, the Larson Gallery in Yakima, and Marks at the Garage in Honolulu. He has also had his work displayed in a variety of shows around the Northwest. His work has won numerous awards including a Silver Award from the Create Chaos Show with this work on display at the Orlando Convention Center and at the Denver airport. It has been featured in Create and advanced Photoshop Magazines. It is rated in the...
Our return policy is very simple:
If you're not happy with a purchase that you made on FineArtAmerica.com, for any reason, you can return it to us within 30 days of the order date. As soon as it arrives, we'll issue a full refund for the entire purchase price. Please note - Fine Art America does not reimburse the outgoing or return shipping charges unless the return is due to a defect in quality.
Fine Art America sells thousands of pieces of artwork each month - all with a 100% money-back guarantee.
If you've hesitated to purchase artwork online in the past, why not give us a try? You have nothing to lose!
We take great pride in the fact that 336,793 artists have chosen Fine Art America to fulfill their orders, and we look forward to helping you select your next piece!
Customer Service: 877-807-5901
(Call Any Time - 24 Hours Per Day)
All canvas prints ship from our production facility within three business days of your order.


text:
10mm Green Shell Pearl and Green Crystal Multi-Strand Necklace. 18"
Item#: 861474 NEW!
Deep and verdant green hues are on display in this multi-strand 10mm green shell pearl necklace, while over 190 green crystals are set in luxurious stations along each of the three strands for a brilliant burst of sparkle. Our shell pearls are lab-created from oyster shells to ensure durability and affordability. Lobster clasp, goldtone necklace.
Product Details (for 10mm Green Shell Pearl and Green Crystal Multi-Strand Necklace. 18")
1-1 1/4 inches
Gold Tone close GOLD TONE: A base or alloy metal that is plated to resemble the appearance of yellow gold. It does not contain any actual natural gold and is frequently found in fashion jewelry.
Polished close POLISHED: A buffed-out finish used to produce a mirror-like shine.
Other Material:
Enamel close ENAMEL: Enamel is a glass-like colored coating which is applied or "painted" over the surface of a piece of metal, ceramic or glass. Enamel is made from powdered glass or an epoxy resin material.
Lobster close LOBSTER CLASP: named after the pinching style of the hook, this clasp is often shaped like a lobster's claw. Use your finger to work the lever to open the clasp.
Gemstone 1:
Simulated Pearl close SIMULATED PEARL: A simulated pearl is an artificial stone made to look like real pearls. Known as "faux pearl," is does not actually hold any of the physical properties of a pearl, as it could be made from glass, acrylic, or plastic. Some are even lab-made from an oyster shell, which gives it an authentic feel and a stronger durability. Typically found in fashion jewelry, faux pearls are incredibly affordable and can create the same elegant look for less. (Color: Green, Quantity: 127, Shape & Cutting Style: Semi-baroque close SEMI-BAROQUE: A pearl with a slightly irregular shape. , Size: 10 mm)
Gemstone 2:
Crystal close CRYSTAL: Though traditionally the term "crystal" was given to glass with a 24% lead content, today it is used in a more general sense to describe transparent and highly refractive glass formed with silica, oxide, or other additives. Crystal is used in serveware, barware, home accents, and jewelry. (Color: Green, Quantity: 192, Shape & Cutting Style: Round, Size: 1.7 mm)
All weights and measures are approximate.
Ross-Simons Presentation Box Your purchase includes an elegant Ross-Simons presentation box.
100% Moneyback Guarantee every item, every order, every day
Free Shipping on orders $250 or more
Only $7.95 Flat Rate for any order under $250
24/7 Live Help toll free at 1-800-835-0919
100% Secure Checkout verified by Norton Security
Recently Viewed Items:
Learn More About Pearls Simulated Pearl Gold Tone Enamel Semi-baroque Pearl Crystal


text:
Smolenak Sent to ChiefsSubmitted by chad on November 6, 2006 - 21:19
The Springfield Falcons are reporting winger Radek Smolenak has been reassigned to Johnstown of the ECHL. Smolenak played two games with the Falcons this past weekend and did not register a point. He was minus-1 in the 2 games. In 6 games with Johnstown this year, he has 4 goals and 2 assists.
- Login to post comments
- 515 reads
Bolt Prospects Prospect of the Week: 10/30-11/5Submitted by pete on November 6, 2006 - 19:34
This one went right down to the wire with great weeks posted by Chris Lawrence (3 GP, 3-2-5), PJ Atherton (4 GP, 0-6-6) and Stanislav Lascek (3 GP, 1-4-5). However, the winner of Prospect of the Week honors is...
NCAA Game Night: 11/5Submitted by Nigel on November 5, 2006 - 21:37
Defensemen, Art Femenella was a +1 with 1 shot on goal in Vermont's 5-2 exhibition win over the University of Ottawa. In 61 career NCAA games at Vermont, the senior has 2 goals and no assists with 146 PIM.
- Login to post comments
- 808 reads
NCAA Game Night: 11/4Submitted by Nigel on November 5, 2006 - 21:13
Mike Lundin had an assist in #1 Maine's 2-2 tie against #8 Boston University. It was Lundin's 3rd assist of the season, and 5th point. He finished a +1 with 3 shots in the game.
Art Femenella was even with 1 shot on goal in Vermont's 1-0 loss to #3 Boston College. The senior defensemen has 1 goal and no assists to go along with 16 PIM in 5 games this season for the Catamounts. Vermont is 2-4-1 overall and 0-1-1 in the HEAST.
Dusty Collins went scoreless with 2 PIM in Northern Michigan's 4-2 upset over #4 Miami-Ohio. The Wildcats moved to 2-2-0 in the CCHA.
UMASS completed the weekend sweep of Providence beating the Friars 3-2.
CHL Game Day: 11/5Submitted by pete on November 5, 2006 - 18:40
Kevin Beech did not dress today as Brampton topped Sudbury 5-4 in overtime.
Chris Lawrence was -1 with 4 PIMs in Mississauga's 6-4 loss to Toronto despite the Ice Dogs outshooting their opponents by a staggering 57-25 margin.
Dane Crowley was scoreless in Swift Current's 4-3 overtime loss to Moose Jaw.
- Login to post comments
- 1066 reads
AHL Game Day 11/5: Falcons at DevilsSubmitted by pete on November 5, 2006 - 18:34
What a big, big win to end a tough weekend set at 2-1-0.
Karri Ramo stopped 23 of 24 shots faced to improve to 3-2-0 on the season. Andy Delmore and rookie Justin Keller had the Falcons' goals, both on the power play, while assists went to Eric Healey, Norm Milley, Mike Egener and Doug O'Brien. The point was Egener's first of the season. Delmore and Keller were named first and second stars of the game respectively.
First Period
LOW Bergfors, (3) (Vrana, LaCouture), 9:54
Second Period
SPR Delmore, (3) (Healey, Milley), 8:18 (PP)
SPR Keller, (2) (Egener, O'Brien), 8:51 (PP)
Third Period
The win moves Springfield to 5-6-0 on the year with 10 points which puts them in 4th place in the AHL's Atlantic Division. Springfield faces the Portland Pirates next Friday night with an opportunity to improve to .500. Portland currently sits in first place in the Atlantic Division.
Game sheet from TheAHL.com.
- 3 comments
- 1048 reads
ECHL Game Day 11/5: Chiefs at NailersSubmitted by pete on November 5, 2006 - 18:24
Morgan Cey must be punchy after this afternoon's game.
Cey stopped 41 of 45 shots faced in the loss as the Chiefs were outshot 45-28 overall. Veteran Randy Rowe posted both of Johnstown's goals with assists going to Adam Henrich and Maxime Boisclair. Wheeling dominated the third period outshooting the Chiefs 18-4 and outscoring Johnstown 2-0 in the frame to take the victory.
First Period
JHN Rowe, (4) (Henrich), 8:39
Second Period
WHE Cheesman, (1) (Bissonnette, Jensen), 6:00 (PP)
WHE Foster, (1) (Cameron, Fernholm), 7:18
JHN Rowe, (5) (Boisclair), 8:06
Third Period
WHE McLean, (5) (Collins, Bissonnette), 3:07
WHE McLean, (6) (Collins), 11:34 (SH)
Game sheet from ECHL.com.
Sunday's Superleague ScoreSubmitted by pete on November 5, 2006 - 18:18
Khimik defeated Salavat Yulayev today 4-2 in the final match before the Karjala Cup break. Alexander Polushin, who has been on IR because of knee surgery, has yet to play for Khimik this season. The Superleague now adjourns for ten days for the Finnish leg of the Euro Tour tournament series. Russia still has not announced their roster as of this posting for the event.
- Login to post comments
- 1246 reads
CHL Game Night: 11/4Submitted by pete on November 4, 2006 - 22:18
Marek Bartanus was -1 in Owen Sound's 5-4 loss to Kitchener.
Vladimir Mihalik had a power play assist and was -1 in Prince George's 4-2 loss to Kelowna. Mihalik also had 4 penalty minutes in the game.
Dane Crowley returned from injury tonight and had an assist and 2 penalty minutes in Swift Current's 5-4 loss to Medicine Hat.
- Login to post comments
- 1073 reads
NHL Game Night 11/4: Lightning at BruinsSubmitted by pete on November 4, 2006 - 22:13
The Lightning were hosed.
Marc Denis allowed 6 goals on 33 shots for the overtime loss. Denis is now 4-0-1 when the Lightning provide him 3 or more goals of support. Vincent Lecavalier led the Lightning attack with 4 assists and Martin St. Louis had 1 goal and 1 assist. Individual goals went to Afanasenkov, Alexeev, Fedotenko and Richards and individual assists went to Kuba, Prospal and Ranger.
If you ever needed proof the league office in Toronto favors their big market, northeastern traditional teams over their small market, southern expansion cousins, witness Zdeno Chara clearly knocking in the game tying goal with a high stick and after a full 10 minutes of review Toronto still blew the call. And, to add insult to injury, Filip Kuba was given a cross check to give Boston a 5-on-3 which they cashed in on in overtime to take the second point. Kudos to the otherwise attrocious Lightning penalty kill for managing to get out of regulation with a point despite being down two men. I won't sit here and say the Lightning played well (they didn't) in this game, but I do know the NHL robbed the team of a point tonight.
First Period
Boston 4:08, Marc Savard 3 (P.J. Axelsson, Glen Murray)
Tampa Bay 12:13, Ruslan Fedotenko 6 (power play) (Vaclav Prospal, Vincent Lecavalier)
Second Period
Tampa Bay 7:15, Brad Richards 3 (power play) (Vincent Lecavalier, Filip Kuba)
Boston 9:08, Marc Savard 4 (Glen Murray, Nathan Dempsey)
Boston 11:03, Phil Kessel 3 (Shean Donovan, Wayne Primeau)
Tampa Bay 11:13, Nikita Alexeev 5 (Martin St. Louis, Vincent Lecavalier)
Tampa Bay 15:38, Martin St. Louis 10 (Vincent Lecavalier, Paul Ranger)
Boston 17:47, P.J. Axelsson 4 (power play) (Marc Savard, Patrice Bergeron)
Tampa Bay 18:14, Dmitry Afanasenkov 2 (unassisted)
Third Period
Boston 19:13, Zdeno Chara 3 (power play) (Marc Savard, Paul Mara)
Overtime Period
Boston 0:17, Glen Murray 6 (power play) (Paul Mara, Marc Savard)
Doug Janik played 8:21 with 1 block and 1 hit. Eric Perrin was -1 in 15:18 with 3 shots and 1 hit and was 63% on draws. Nick Tarnasky was -1 in 1:59 and 0/1 on draws.
Game sheet from NHL.com.
- 1 comment
- 716 reads


text:
from where I can able to get the ticket for these type of fights
Thanks for helping us associate the correct Pornstars to this video!
Help Us: Do these Pornstars appear in this video?
Pornstar Name:
Thanks for helping us sort this video!
All Comments (21)
from where I can able to get the ticket for these type of fights
did anyone get the rules? :)
sound like a turkey right at the end!
wow this is actually considered a sport when can i try out!!
the men need to get those dicks out and get in there
Love it hardcore lesbian action
This is so stupid
This is made like literally 6 blocks from house in sf
anyone else see the stat diff ami had like 2 2 vendetta had like 46 6 LOL
where do i get tickets to these kind of fights ? Or maybe they're on pay per view!!!! lol
why about the blonde on the blue team?
Claire Dames
looks like a great sport need to get it started in adelaide...
Guys this is a win, win situation for them... Cuz if they loose they get pounded "hard" And Everyone of them always have a orgasim.
they have make an olympic sports out of it.
You can tell the blue is gonna lose from the beginning and when they do its hot.
Whats the name of the girl in the blue team with the big tits


text:
Hours of Operation
Monday, Wednesday,
Thursday 9am-noon
Main Entry for Meeting Room and Gift Shop at the
Dale Shaffer Research Library
239 South Lundy
Salem, OH
Sundays Tours 1-4pm
(May through October)
Tours begin at the rear door of the Pearce House in the old meeting room on Shaffer Way.
The Salem Historical Society
Mailing Address:
208 South Broadway Ave
Salem, OH 44460
(330) 337 - 8514
....keeping and making history
Lost? Download our Campus Map


text:
RedSub BCH-2 Stereo Bass Chorus Pedal
RedSub BCH-2 Stereo Bass Chorus PedalDetails
Along with the standard Rate, Depth and Level controls, the RedSub BCH-2 Bass Chorus Pedal also features a low filter for cutting or boosting the bass tones, stereo or mono outputs for making a huge sweeping chorus sound, and a choice of either battery power or AC adaptor.
Product Ref: 22206
- Stereo Or Mono Output
- Rate, Depth and Level Controls
- Low Cut Filter
- Rugged Steel Case
Full DescriptionView Full Description
Command Your Sound With Low Filter Control & True Bypass
The RedSub BCH-2 Stereo Bass Chorus Pedal is another in the RedSub pedal series, designed especially for bass. Featuring a rugged high grade steel casing with rubber protectors, 9v AC or battery power, an easy access battery compartment and standard jack input and outputs, this pedal represents fantastic value for money.
The RedSub BCH-2 Stereo Bass Chorus pedal offers a wide range of sounds, from a gentle swell to a full 1980's 'Twin Peaks' sound. Along with the standard Rate and Depth controls, the BCH-2 also features a low filter control, meaning you can control how bassy your chorus sound is. It also features both a mono and a stereo output, so you can have a full stereo chorus effect.
RedSub is a premium brand of Gear4music.com
RedSub manufactures premium-level bass guitar amplifiers using high-grade components, quality craftsmanship, custom speakers and the latest in analogue and digital bass amp technology.
Providing distinctive looks, as well as the professional features found on much higher price tag amplifiers, Redsub deliver the ultimate in value, power, looks and sound.
With a power range from 35w through to 500w; be it practice or performance, a RedSub amp will meet the measure. RedSub bass amps bring a new level of quality and affordability to the professional bass guitar amp market.
The product price includes a one year warranty and 30 day money back guarantee.
However, the Gear4music extended warranty scheme provides an enhanced service, and is designed to offer a higher level of protection than many 'free' warranties offered by some retailers and manufacturers.
The highlights of this enhanced warranty service are:
- Warranty is extended to 5 years from the date of delivery.
- Our Money Back guarantee is extended from 30 days to 90 days (terms apply after 30 days)
- Collection and delivery costs are included for the first two years
- Claim 50% cash back on the cost of the policy after 5 years if it has not been used!
- Warranty is transferable if the item is re-sold
- Loan or replacement equipment will be provided if the product isn't repaired within 30 days
- If an item can not be repaired, a similar alternative product or cash discount (as defined in this policy) will be offered
- The policy can be cancelled within 30 days, and at any time after that for a pro-rata discount
Please note that this extended warranty is currently only available on products sold within the EU or EEA, and is subject to the terms and conditions as detailed on the extended warranty certificate provided.
This enhanced warranty is in addition to the standard Gear4music and manufacturer warranty policies, and the Gear4music terms and conditions of trade. No statement or condition of sale contained within these terms affects or restricts your statutory rights as defined under The Consumer Contracts (information, Cancellation and Additional Charges) Regulations 2013.
Once the product is delivered, you have 30 days to decide that you definitely want to keep it. If you decide it is not suitable for any reason, you can return it back to us for replacement or full refund, including standard delivery charges.
All Products are new unless stated, and include a minimum of one year warranty.
Some items are only eligible for money back guarantee if they are returned to us unused and sealed in the original packaging, and in the case of software, unregistered. These items include:
- Software
- CDs & DVDs
- Headphones and In-ear Monitors
- Earplugs and Ear Protection
- Mouthpieces, Reeds etc… for Woodwind and Brass instruments
- Mouth / Lip Care Products
We are unable to accept returns of any custom made items designed to work together as a unique package, such as computer system built to a customer's personal specifications. Exceptions may only be possible with prior agreement of the vendor, and may be subject to a cancellation fee.
If you also purchase items that do not qualify for free delivery, some delivery charges may be applied to your order which will be shown in your shopping basket.


text:
Designer Robin Harris created a fashion line for statuesque women, like herself, because she's tall and she's not apologizing for it. [read more]
The EBONY "Sexy" issue isn't the only cover Kelly Rowland heats up, she celebrated her Vegas Magazine's cover… Read More
Typically couples aren't able to ask each other, "Whose movie premiere are we heading to tonight babe, yours or mine?"… Read More
We are not "old Black" nor "new Black" we are ALL Black.
Out there in this world are strong Black men who work hard and play harder, rear their children while comforting their mates, and… Read More
Muhammad Ali will be the next Louis Vuitton icon. Print ads featuring the boxing champion and global icon… Read More
We nearly lost it when rumor had it that Will and Jada were packing their bags and heading for Splitsville after 14 years of marriage and have been… Read More
We challenged you to pack light for your next voyage, but now it is time to envision yourself unwinding on a white sand beach with the warm salt water… Read More
It seems as if the fashion industry still has its eyes set on Africa. Known for jump-starting the successful careers of several… Read More
To wear a skirt or to not wear a skirt? That is the question that comes to mind when the weather is warm and work calls. If… Read More
20 years ago, the idea of wearing suspenders, bowties, vests and glasses were more Steve Urkel. Traditionally, men dressed in this style were considered… Read More
We are not "old Black" nor "new Black" we are ALL Black.
Vernon Reid Talks 30 Yrs of the Black Rock Coalition
Justin Adu Explains African Amedia
Beautifully Brown Grace Jones
Why Did Ben Carson Mock Police Brutality
Detroit's New Activist Generation
by Shantell E. Jamison
by Shantell E. Jamison
by LaToya Cross
by Jazz Keyes


text: This hotel was clean and charming. The owners were helpful . We arrived late but were still able to have dinner in the dining room. I was only here 1 night but would have stayed longer. There was no A/C at first was concerned but with the balcony door opened it was fine. Everything was fine, nice room, good food.


text:
Mario Cake
- Uploaded By tonilynn
- 2010-06-19 16:42:57
I used two 10" rounds for the bottom layer and two 6" rounds for the top. Butter cream frosting tinted with sky blue. I used a cloud cookie cutter for the clouds in fondant and free handed all the other details. I piped grass hills using star tip and I purchased the mini figurines on ebay.
No Comments Yet
Login To Leave A Comment


text:
This job posting is no longer available on Indeed.
Find similar jobs:Neuropsychologist jobs
- Renee Applebaum jobs
Full time position available for Neuropsychologist to join busy private practice in Bingham Farms, MI. Position requires neuropsychological evaluation and individual psychotherapy with persons with tbi, dementia, vascular disorders, movement disorders and other neurological insult. Candidates should have substantial experience in neuropsychological evaluation and should be fully licensed or eligible for full license in the State of Michigan. Competitive salary is offered. Only qualified individuals should submit a CV and letter of interest.
Email this job to a friend


text:
My name is Zachary. I'm a pretty easy going guy who likes dive bars and a cheap Thai place over clubs and expensive restaurants.
Join OkCupid
Find better matches with our advanced
Find better matches with our advanced
matching system
—% Match
—% Enemy
26 • Goodrich, MI • Man
I'm looking for
- Men
- Ages 19–35
- Near me
- Who are single
- For long-term dating, short-term dating
My details
- Last online
- Yesterday – 11:42am
- Orientation
- Gay
- Ethnicity
- White
- Status
- Single
- Relationship Type
- Monogamous
- Height
- 5' 6" (1.68m)
- Body Type
- Average
- Smoking
- No
- Drinking
- Often
- Drugs
- Never
- Sign
- Capricorn
- Education
- University
- Offspring
- Wants kids
- Speaks
- English
My self-summary
Write a little about yourself. Just a paragraph will do.
What I'm doing with my life
Don't overthink this one; tell us what you're doing day-to-day.
I'm pursuing a career in hospitality. Coming back to the Detroit area from Las Vegas to get a fresh start. Looking forward to reconnecting!
I'm really good at
Go on, brag a little (or a lot). We won't judge.
Laughing and getting a laugh.
The first things people usually notice about me
I'm an empty essay… fill me out!
My eyes. I'm always asked if I'm wearing eye liner.
Favorite books, movies, shows, music, and food
Help your potential matches find common interests.
I love scary movies (I think I have single handedly survived Redbox's horror section). I have every season of the golden girls - judge if you must. I don't have a tv so I do read often and always open to suggestions for good reads!
The six things I could never do without
Think outside the box. Sometimes the little things can say a lot.
Good Friends
A Good Book
Good Friends
A Good Book
On a typical Friday night I am
Netflix and takeout, or getting your party on — how do you let loose?
Hanging out at home and probably falling asleep early.
You should message me if
Offer a few tips to help matches win you over.
You're genuine and think a little crazy just makes things more fun :)
Report this photo
How does this violate our Photo Rules?
You must be in the photo! Full nudity, extreme close ups, pets, cars, baby photos, artwork, images you've added yourself to, etc. are not allowed.
Photos in albums are meant to showcase other aspects of your life, and may include pets, cars, baby photos, artwork, etc. Full nudity and offensive content are not allowed.
Thanks! We'll look into the issue soon.
Flagging inappropriate content keeps OkCupid awesome. You're now eligible to be a flag moderator!
Connect your existing OkCupid account
Sign in
Welcome Back!
Restore your account to continue
meeting new people.
Reset your password
We'll email you a link to reset it.
Email sent!
An email is on its way to .
If you don't see it, try checking
your Spam folder.


text:
Select Local Merchants
Timlin's FurnitureRead MoreWhether you're decorating a dorm room, an apartment or a home, Timlin's Furniture is a must-see furniture store in Hartford. When you are ready to add some new pieces to your living room, shop the selection of furniture here, such as living room furniture. If you're looking to make your bedroom more comfortable, check out the furniture options here, such as dressers, bed frames, and bedroom furniture. Brand new furniture is a great way to clean up a workspace that's just a tad too messy. Deck out your bathroom with brand new furniture and transform your home in a pinch. What goes hand in hand with summer and sun? Patio furniture! Check out all the latest and greatest offerings for the best backyard on the block. Mattresses and mattress pads and covers are just some of the many affordable and comfortable bedding options that this store has to offer. If you're in need of some design tips, head on over to this store and peruse the exquisite pieces on display. Quality decorations go a long way when it comes to making a home your own, so start with rugs, throw pillows, and vases. If your walls are looking a little bare, spruce them up with items like curtains and mirrors. Convenient parking spaces are located nearby to Timlin's Furniture. If you're on the market for some affordable and trendy furniture, Timlin's Furniture in Hartford has you covered.5980 State Road 60Hartford, WI US
Lincoln Tropical Fish and PetsRead MoreThe knowledgeable and friendly staff at Lincoln Tropical Fish and Pets in Hartford can help you find everything you need for your new furry friend. This store has everything you need like carriers so you can keep your pet happy and healthy. Treat your pet to a new tasty meal from here, such as bowls and other feeders, and keep them happy all day long. Keep your pet entertained for hours with all the fun toys in stock, including pet apparel and pet toys. Every pet deserves a comfortable place to call home. Lincoln Tropical Fish and Pets has a wide variety of pet apparel and pet toys to choose from! A happy pet is a healthy pet! Check out the many health services offered at Lincoln Tropical Fish and Pets, such as healthcare. If you prefer to commute by car, take advantage of the many parking options in the area. You'll love the comfort afforded to your pets when you buy new bedding and other accessories from Lincoln Tropical Fish and Pets in Hartford.10 S Main StHartford, WI US
Cloud NineRead MoreThe staff at Cloud Nine in Hartford is here to help you gather supplies and attire for your new family pet. Give your pet a comfy little abode of their own. You'll find Cloud Nine has an extensive selection of pet apparel and pet toys to choose from. Make sure your pet gets all the nutritious food they need with some of their quality feeding supplies, including bowls and other feeders. You will find a bunch of great items that your pet will love to play with including pet apparel and pet toys. Think your pet needs a health check? There are plenty of medical services available, including healthcare. Your pet deserves the best. Pick up some supplies, such as carriers, and other goodies to keep them healthy and happy. No matter what time of day you visit Cloud Nine, you can find easy in-and-out parking for your hot ride. Help your pet live their best life following a glamorous trip to Cloud Nine's pet store in Hartford.3336 County RdHartford, WI US
Butler Creek KennelsRead MoreButler Creek Kennels in Hartford is a high-quality pet store that has everything from pet food to toys. Make sure your pet has all the food they need to stay healthy and strong by picking up some great feeding supplies from here, such as bowls and other feeders. Your pet will love you if you bring it home an item like pet apparel and pet toys. Every pet deserves a comfortable place to call home. Butler Creek Kennels has a wide variety of pet apparel and pet toys to choose from! If you're bringing a new pet home soon, grab some basic supplies such as carriers and have everything you need on hand. There are plenty of services available to ensure your pet's well-being, such as healthcare. Heading to Butler Creek Kennels? Take the whole family along for a fun shopping trip and find easy parking nearby. It's time for a little more love in your home by heading to Butler Creek Kennels for a great new pet.W720 Buchanan RdHartford, WI US
Logan's Pet Grooming and DaycareRead MorePet heaven is located right at Logan's Pet Grooming and Daycare in Hartford. Here you'll also find a wide variety of services to ensure your pet is healthy and happy, like healthcare. If you just added a new addition to your family, you can pick some basic pet supplies such as carriers here. Items like pet apparel and pet toys will keep your pet entertained all day long. You know how important it is to keep your pet well-fed and happy, so pick up some of their quality feeding supplies, like bowls and other feeders. Every pet deserves a comfortable place to call home. Logan's Pet Grooming and Daycare has a wide variety of pet apparel and pet toys to choose from! There's great parking only a short distance from Logan's Pet Grooming and Daycare. So keep your pet happy and entertained for hours with a brand new toy from Logan's Pet Grooming and Daycare in Hartford.2962 State Road 83Hartford, WI US
Friends of The Nature CenterRead MoreAnything you need for a pet can be found at Friends of The Nature Center in Slinger. If you're on the market for some new pet supplies, such as carriers, you can find a great selection of supplies here. Give your pet the meal of a lifetime with items such as bowls and other feeders, and watch them leap with joy. This store knows how important your pets are to you and offers services such as healthcare. Your pet will love you if you bring it home an item like pet apparel and pet toys. Make sure your pet has a comfortable place to call home. Check out Friends of The Nature Center's large selection of pet apparel and pet toys. With a parking lot adjacent to Friends of The Nature Center, you won't get stuck circling the block. If you're in need of some pet supplies, head on over to Friends of The Nature Center in Slinger and purchase your necessities.433 E Washington StSlinger, WI US


text:
Dean Townes' insights from September's E-Newsletter, Spire
Dear alumni/ae and friends,
One of my favorite parts of the new school year is the opportunity to engage with so many noted scholars, writers, artists and others who come to the Divinity School to inspire and often challenge us in our personal and spiritual journeys.
I hope that you will be able to join us for many of these talks, especially the ones by Nikky Finney, our 2015 Cole Lecturer. Finney, whose Head Off & Split won the National Book Award for poetry in 2011, grew up in South Carolina during the civil rights and black arts movements. She returned to her home state in 2013 to be the John H. Bennett Jr. Chair in Southern Letters and Literature at the University of South Carolina.
Read more here
VDS Voices Blog
- Alumni/ae Tuesday – Khette Cox, MDiv'09 — Our monthly Alumni/ae Tuesday Guest Post series on the VDS Voices blog highlights posts written by VDS and GDR alumni/ae. Hear firsthand about their important work in the community, collaborations with other alumni/ae and faculty, and much more. Be sure to also check out the Divinity School Instagram feed every Tuesday for our Alumni/ae Instagram Takeover Day....October 13, 2015
- READ THIS BOOK – October 2015 — Each month, we ask a member of the Vanderbilt Divinity School faculty to recommend a book they are currently reading. Our October recommendation is offered by Paul DeHart, Professor of Theology and Chair, The Graduate Department of Religion. Peter Brown, Through the Eye of a Needle: Wealth, the Fall of Rome, and the Making of Christianity...October 11, 2015
- Imagination Grant: Community Engagement and Religion in Yaxhachen, Yucatán, Mexico — The Imagination Grant seek to advance global learning, theological reflection, and leadership skills and support creative, non-credit bearing projects proposed by students. The funds can be used to cover travel and expenses for global immersion experiences, and are typically utilized during summers. by Chelsey Overstreet Hedglin, 2nd Year MDiv This summer, I was given the opportunity...October 7, 2015
- More from VDS Voices»
News from VDS
- What would Jesus say about same-sex marriage? Vandy prof, Amy-Jill Levine answers October 9, 2015
- VDS alumna Blair Tolbert, MDiv'10 named Dean of Chapel & College Minster at Young Harris College October 1, 2015
- Professor Choon-Leong Seow presents "Consequences of Scripture: the Case of Job" as part of the Gunning Lecture Series at The University of Edinburgh September 30, 2015
- More News »


text:
Royalty Free Music Unique To Your Project - Every Time
Royalty Free Music — Unique To Your Project – Every Time
Try SmartSound's Award Winning and Patented Technology Yourself
New Royalty Free Music Search
Or Browse By (Click to add multiple browse filters)
Genre | Instrument | Keyword | Advanced
Royalty Free Music Search Results (You are in TRACK VIEW. Switch to ALBUM VIEW)
The following tracks match: Nostalgic, Kids
Hopping Alonghttps://www.smartsound.com/strata/images/STRATFG_200.jpgSingle70904-ST$ 39.9570904-EX$ 69.9570904-W$ 149.95Genre: Nostalgic, Country/Folk, Pop Production Types: Kids, Commercials, Comedy, Home Videos Keywords: Tongue-in-cheek, Funny, Gentle, Goofy, Innocent, Wacky, Whimsical, Fun Instrument: Mandolin, Violin, Violoncello, Acoustic, Instrumental
Hopping Along from the album Fun & Games
Bright SunshineThis mellow, laid back track is ideal for commercials displaying morning or upbeat imagery. Featuring whistling melody and acoustic guitarhttps://www.smartsound.com/strata/images/STRATGSS_200.jpgSingle69303-ST$ 39.9569303-EX$ 69.9569303-W$ 149.95Genre: Nostalgic, Retro, Vintage, Whistling, Specialty Production Types: Kids, Commercials, Comedy, Home Videos Keywords: Tongue-in-cheek, Carefree, Heartwarming, Old-Fashioned, Tender, Wacky, Whimsical, Angelic Instrument: Vocal
Bright Sunshine from the album Goofy, Silly & Sweet
Evening by the FireplaceIdeal for night time imagery or a couple sharing a romantic conversation, this mellow tune features a recorder melody, electric piano & acoustic guitar.https://www.smartsound.com/strata/images/STRATGSS_200.jpgSingle69304-ST$ 39.9569304-EX$ 69.9569304-W$ 149.95Genre: Nostalgic, Specialty Production Types: Kids, Commercials, Comedy, Home Videos Keywords: Calm, Carefree, Comical, Heartwarming, Soothing, Tranquil, Wacky, Quirky Instrument: Flute, Guitar (Acoustic), Instrumental, Guitar
Evening by the Fireplace from the album Goofy, Silly & Sweet
Whistler's DreamThis soothing tune will make you feel like you're walking on a beach. Featuring a whistling melody and tuba.https://www.smartsound.com/strata/images/STRATGSS_200.jpgSingle69310-ST$ 39.9569310-EX$ 69.9569310-W$ 149.95Genre: Nostalgic, Whistling, Specialty Production Types: Kids, Commercials, Comedy, Home Videos Keywords: Tongue-in-cheek, Carefree, Comical, Goofy, Heartwarming, Refreshing, Whimsical, Quirky, Angelic Instrument: Accordion, Mandolin, Piano (Electric), Tuba, Instrumental
Whistler's Dream from the album Goofy, Silly & Sweet
Daisy's ParadeThe good times tumble right out of this slaphappy tune, as if from the rickety player piano in the corner. It could be the theme song for a pair of bumbling bank robbers, or any wild character in your story.https://www.smartsound.com/producer/images/ps20_200.jpgSingle32005-ST$ 39.9532005-EX$ 69.9532005-W$ 149.95Genre: Nostalgic, Specialty Production Types: Kids, Animation, Comedy, Home Videos Keywords: Happy, Carefree, Cheerful, Comical, Funny, Old-Fashioned, Whimsical, Fun Instrument: Dixie Band, Instrumental
Daisy's Parade from the album Comical / Fun / Novelty (PS20)
Ragtime SpoofPut a skip in your step with this sunny, stride piano number. A great theme song for that happy-go-lucky character. Also features zany punctuation from slide whistle and harmonica.https://www.smartsound.com/producer/images/ps20_200.jpgSingle32014-ST$ 39.9532014-EX$ 69.9532014-W$ 149.95Genre: Nostalgic, Specialty Production Types: Kids, Animation, Comedy, Home Videos, Background Keywords: Energetic, Happy, Tongue-in-cheek, Cheerful, Goofy, Old-Fashioned, Whimsical, Sprightly, Fun Instrument: Solo, Piano, Instrumental
Ragtime Spoof from the album Comical / Fun / Novelty (PS20)
The following tracks match: Nostalgic
Empty SurpriseWarm and reflective with light percussion and bass offer a sentimental and introspective mood.https://www.smartsound.com/strata/images/STRATUBU_200.jpgSingle7100210-ST$ 39.957100210-EX$ 69.957100210-W$ 149.95Genre: Nostalgic, Various Production Types: Commercials, Documentary, Background Keywords: Mysterious, Atmospheric, Calm, Gentle, Hypnotic, Intimate, Pensive, Reflective, Relaxed, Ambient, Mellow, Investigative, Magical, Quiet, Soft Instrument: Bass (Acoustic), Drums, Piano, Piano (Electric), Synthesizer, Instrumental
Empty Surprise from the album Upright Bass Underscores
Silver DustPiano swells, sensitive strings and driving drums build this creatively composed tune to a victorious crescendo.https://www.smartsound.com/strata/images/STRATCPB_200.jpgSingle7100195-ST$ 39.957100195-EX$ 69.957100195-W$ 149.95Genre: Nostalgic, Rock, Indie Rock, Indie Artist, Rockin, Pop Rock, Pop Production Types: Commercials, Indie Film, Home Videos, Background Keywords: Energetic, Exciting, Uplifting, Inspirational, Dramatic, Fresh, Heartwarming, Inspiring, Motivating, Passionate, Pensive, Powerful, Punchy, Touching, Triumphant, Hopeful, Emotional, Poignant Instrument: Bass (Electric), Guitar (Acoustic), Guitar (Electric), Piano, Strings, Instrumental, Guitar
Silver Dust from the album Contemporary Pop Beds
A Champion's StartA gentle acoustic guitar leads a mesmerizing and lush production which floats expansively into a harmonica and shaker-led melody.https://www.smartsound.com/artist/images/ASMR02_200.jpgSingle7100146-ST$ 39.957100146-EX$ 69.957100146-W$ 149.95Genre: Earthy, Elegant, Nostalgic, Outdoorsy, Processional, Sophisticated, Country/Folk, World, Exotic, Indie Artist, New Age, Irish, Indie, Medieval Production Types: TV/ Film Drama, Commercials, Documentary, Film Trailer, Biography, Background Keywords: Positive, Inspirational, Atmospheric, Calm, Delicate, Ethereal, Fresh, Gentle, Heartwarming, Inspiring, Intimate, Majestic, Motivating, Panoramic, Pensive, Reflective, Refreshing, Serene, Soothing, Tender, Touching, Tranquil, Angelic, Emotional, Poignant, Soft, Folklore Instrument: Guitar (Acoustic), Percussion, Uilleann Pipes, New Age Ensemble, Acoustic, Instrumental, Guitar
A Champion's Start from the album Captivating Portraits
Breathless-NTDelicate piano, strings and woodwinds lend a romantic, tantalizing and yearning feel to this engaging track.https://www.smartsound.com/strata/images/STRATOH_200.jpgSingle7100156-ST$ 39.957100156-EX$ 69.957100156-W$ 149.95Genre: Nostalgic, Orchestral, Waltz Production Types: Cinematic, Documentary, Indie Film, Film Trailer, Biography, Home Videos, Spoken Word/Narration, Tribute/Memorial, Background Keywords: Inspirational, Delicate, Dramatic, Gentle, Heartwarming, Inspiring, Intimate, Light, Melancholy, Refined, Reflective, Serene, Uncertain, Mellow, Emotional, Quiet Instrument: Orchestra, Studio Orchestra, Oboe, Piano, Strings, Instrumental
Breathless-NT from the album Orchestral Hues
Choose your license:
|Customizable with Sonicfire Pro and Quicktracks
|Personal and professional use
|Promote a product or service
|Use on my own website
|Videos created for a third party
|Use on my clients website
|Monetized videos on YouTube, Vimeo, Facebook, Etc.
|Display at Film Festivals
|Display in theaters
(one theater)
(unlimited theaters in one country)
|Assign Distribution Rights
|DVDs & Downloads
(up to 2000)
(up to 10000)
|In mobile apps
|Local TV/radio broadcasting (includes advertising)
|National TV/radio broadcasting (includes advertising)


text:
|Publication number
|Publication type
|Application number
|Publication date
|May 28, 1996
|Filing date
|Jan 24, 1994
|Priority date
|Jan 24, 1994
|Fee status
|Also published as
|Publication number
|Randel E. Dorian, Kent C. Cochrum
|Original Assignee
|The Regents Of The University Of California
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (17), Non-Patent Citations (4), Referenced by (15), Classifications (42), Legal Events (8)
|External Links: USPTO, USPTO Assignment, Espacenet
This application is related to copending application entitled "Multiple Layer Alginate Coatings of Biological Tissue for Transplantation", Ser. No. 08/186,327, filed on Jan. 24, 1994.
1. Field of the Invention
The present invention concerns a microcapsule generating system and relates to encapsulation of material generally, and more particularly to encapsulating tissue or a suspension of cells so that the encapsulated tissue or cells remain viable within a protective membrane or coating. The membrane or coating is permeable to nutrients, ions, oxygen, and other materials needed both to maintain the tissue and to support its normal metabolic functions, but is impermeable to bacteria, lymphocytes, and large proteins of the type responsible for immunological reactions resulting in rejection.
Insulin-producing or other hormone-producing systems, cells from tissues, primary cultured cells, cultured cell lines that produce biological products of interest (such as Factor VIII and calcitonin), and genetically engineered cultured cell lines, for example, can be coated using the encapsulating apparatus of the present invention. That is, this apparatus permits encapsulation of mammalian pancreatic beta cells, alpha cells, intact islets of Langerhans, and other tissues or tissue fractions which secrete hormones. The encapsulated cells or tissue may be suspended in a culture medium where they will secrete hormones over an extended period.
2. Background Art and Related Art Disclosures
Various attempts have been made to provide semipermeable microcapsules that were both biocompatible with the body tissue and impermeable to the components of the immune system. Typically, living tissue or individual cells are suspended in an aqueous solution of a reversibly gelable material, such as sodium alginate, and droplets of this suspension are formed and allowed to fall into a gelling solution, such as calcium chloride. The temporary capsules so formed are then treated with a crosslinking polymer, such as polylysine and polyethyleneimine, to form an outer semipermeable coating.
The droplets are typically formed by feeding the alginate suspension to a first site where a mass of the liquid suspension accumulates. Then the mass of liquid suspension is agitated such that it is broken up into small droplets. Devices using vibration, centrifugal force, air currents and electrostatic charges have been used to agitate the liquid to generate the small droplets.
One drawback of conventional devices using vibration, centrifugal force and air currents is that the diameters of the microcapsules produced thereby are dependent on the sizes of the orifices through which the suspension is extruded and are typically 500 μm or greater with devices used for pancreatic islet encapsulation, where a relatively large bore diameter is dictated by the large size of islets (50-300 μm). Since oxygen diffusion is insufficient to maintain cell viability at distances exceeding about 150 μm, the cells in the center region of these microcapsules are routinely lost due to oxygen deprivation.
Although devices using electrostatic charges are claimed to produce microcapsules having diameters as small as 150 μm (see, e.g., U.S. Pat. No. 4,789,550 to Hommel, et al.), the blank microcapsules and microcapsules containing cells or tissue produced by electrostatic devices generally are the same size. Accordingly, the separation and differentiation between the blanks and the other microcapsules is difficult at best. In addition, in order to produce microcapsules of small diameter using this approach, it is necessary to use small bore needles which cannot accommodate the larger particles in the suspension and which tend to clog with high density cell or other particulate suspensions.
The present invention is directed to a microcapsule formation device that significantly minimizes the problems and disadvantages presented in conventional devices. The invention accomplishes this goal by providing a microencapsulation system with a droplet-forming air knife that includes a capillary tube or needle and an air sleeve. The capillary tube or needle is adapted for coupling to a first source of fluid, such as an alginate suspension, from which droplets are to be formed. The air sleeve is adapted for coupling to a second source of fluid, for example, sterilized air. The discharge end of the capillary tube or needle is positioned in the immediate vicinity of the discharge end of the air sleeve so that air currents from the air sleeve increase the force acting on a nascent droplet of the first fluid at the discharge end of the capillary tube or needle and help break the droplet away from the needle to free fall into a gelling solution. That is, as the liquid suspension of the materials to be coated or encapsulated is discharged from the capillary tube or needle, pressurized air introduced into the air sleeve breaks the liquid suspension into tiny droplets.
According to a first embodiment of the invention, the center of the outlet opening of the needle is offset from the center axis of the air sleeve. Thus, the needle can be eccentrically positioned within the air sleeve of the needle with its outlet opening eccentrically positioned relative to the center axis of the sleeve, for example. It has been found that the eccentricity of the needle outlet and air sleeve enhances the ability of the device to produce very small droplets. In addition, it has been empirically determined that the greater eccentricity of the capillary tube or needle (or its outlet), the smaller the droplet size.
According to a second embodiment of the invention, the capillary tube has a beveled, pointed discharge end. This configuration also has been empirically shown to be important to the generation of very small droplets.
According to a further advantageous feature of the present invention, the entire beveled portion of the needle is positioned beyond the end of the air sleeve. It has been empirically determined that the optimal needle position for producing the smallest possible droplets is when the uppermost region of the beveled portion of the needle is spaced a very short predetermined distance (e.g., 1 mm) from the air sleeve outlet.
The air knife of the present invention described above can form very small alginate droplets containing suspension of individual cells or tissue. The droplets are sufficiently small so that upon contact with a gelling solution, such as CaCl2, microcapsules are formed having diameters from about 20-300 μm, depending on the size of the tissue or cells being encapsulated. This is especially advantageous when the microcapsules are to be introduced into a patient. That is, the volume of material being introduced into the patient can be reduced since the membrane is close fit about the encapsulated biological material. The small size of the microcapsules also makes delivery to the patient less intrusive as smaller needles are required for injection into the patient. Since the microcapsules are less than about 300 μm in diameter, diffusion of oxygen to the center of the capsules is no longer a problem. It also has been found that blanks (microcapsules without cells or tissues), which are formed in the present encapsulation process, are about ten to fiftyfold smaller than the microcapsules containing cells or tissue and, thus, are readily distinguishable and separable from the encapsulated cells and/or tissue.
Another especially advantageous aspect of the device of the present invention is that it can be used to form a thin second coating on encapsulated cells or tissue while maintaining the diameter of the double or multiple-coated microcapsule within 10-40 μm of the diameter of the single-coated microcapsule. The second coating is formed by passing a suspension of the encapsulated cells or tissue in alginate solution through the needle while introducing pressurized air into the sleeve. The additional coating(s) ensure(s) that the cells or tissue are completely encapsulated.
A method of forming droplets according the invention is also disclosed. The method includes providing first and second tubes each having an outlet opening, positioning the outlet openings with respect to one another such that the second tube outlet opening is located in the flow path of the gas discharged from the tube outlet opening, the center of the second tube outlet opening being offset from the center axis of the first tube outlet opening causing pressurized gas to flow from the first tube outlet opening, and causing a fluid to flow from and be suspended from the end of the second tube outlet opening so that the gas flowing from said first tube outlet opening impinges fluid suspended from the second tube outlet opening to form droplets therefrom.
The above is a brief description of some deficiencies in the prior art and advantages of the present invention. Other features, advantages and embodiments of the invention will be apparent to those skilled in the art from the following description, accompanying drawings and appended claims.
FIG. 1 is a diagrammatic elevational view of a microcapsule generating system in accordance with the principles of the present invention;
FIG. 2 is a diagrammatic elevational view of a further embodiment of the microcapsule generating system of FIG. 1;
FIG. 3 is an enlarged sectional view of the distal ends of the air knife discharge tubes used in the microcapsule generating systems of FIGS. 1 and 2, showing the inner tube or needle arranged such that it abuts against the inner surface of the outer tube or sleeve;
FIG. 4 is a sectional view taken along line 4--4 in FIG. 3;
FIG. 5 is a side elevational view of the inner tube of FIG. 3 shown rotated 90 degrees;
FIG. 6 is an enlarged sectional view of the distal ends of the air knife discharge tubes of FIGS. 1 and 2 showing the inner tube or needle arranged such that it is radially spaced from the inner surface of the outer tube of sleeve;
FIG. 7 is a sectional view taken along line 7--7 in FIG. 6;
FIG. 8 is an enlarged sectional view of the distal ends of the air knife discharge tubes of FIGS. 1 and 2, showing the outer tube beveled according to another embodiment of the invention;
FIG. 9 is a further view of the beveled sleeve and needle of FIG. 8 with the outer tube shown rotated 90 degrees;
FIG. 10 is a graph illustrating the size distribution of single-coated microcapsules formed from three different preparations of hepatocytes under the same procedures using the system illustrated in FIGS. 1 and 3-5 (90% of the microcapsules have a diameter less than 75 μm);
FIG. 11 is a graph illustrating the size distribution of single-coated microcapsules containing a proliferating cell line that provides Factor VIII using the system illustrated in FIGS. 1 and 3-5; and
FIG. 12 is a graph illustrating the size distribution of single-coated microcapsules containing a proliferating cell line that secretes calcitonin.
Referring to the drawings in detail wherein like numerals indicate like elements, a microencapsulation system is shown in FIG. 1 according to the principles of the present invention. Although the present invention can be used to encapsulate other materials, it will be described in conjunction with the encapsulation of droplets of an alginate suspension containing individual cells or tissue for purposes of simplification.
Referring to FIG. 1, microencapsulation system 1 generally comprises an air knife 2, which forms droplets 34 containing individual cells or tissue suspended in a gelable polymer solution, and a collection vessel or tank 4, which contains a gelling solution 36. Gelling solution 36 is positioned below the air knife for collecting the droplets 34 and causing microcapsules 38 containing the desired biological material to be formed.
Air knife 2 includes a syringe 6 for dispensing the material to be encapsulated, such as an alginate solution 7, and a tubular air sleeve 26 which will be described in more detail below. The syringe 6 includes a barrel 8, which is shown as containing the alginate suspension 7, a needle assembly 10, and a plunger 12 for forcing the alginate suspension through the needle assembly. Needle assembly 10 includes a capillary tube or needle 14 and hub 16 which fluidly couples needle 14 to barrel 8, so that fluid, such as alginate solution 7 can be dispensed from barrel 8 through needle 14.
Plunger 12 includes a piston 18 and a stem 20 which are interconnected so that piston 18 can be readily moved to displace alginate suspension 7 from barrel 8 into and through needle 14. A grip 22 and a finger ledge 24 also are provided as is conventionally known in the field, to facilitate the manual displacement of plunger 12. Preferably, a mechanical drive (not shown) can be coupled to the plunger to displace the plunger at a constant rate as would be apparent to one of ordinary skill.
Referring to FIGS. 1 and 3-5, tubular air sleeve 26 is positioned around part of the axial length of needle 14, i.e., needle 14 extends through sleeve 26. Sleeve 26 includes an end wall 27 through which needle 14 extends. End wall 27 and needle 14 form a closed end for sleeve 26 which includes an open distal end 30. A feed pipe 28 is fluidly coupled to air sleeve 26 for introducing pressurized gas, preferably sterilized air, into the flow path or space 53 formed between the outer wall surface of needle 14 and the inner wall surface of air sleeve 26. As will be described in more detail below, the pressurized air in air sleeve 26 controls, in part, the size of the droplets dispensed by needle 14. Air knife 2 is suspended above collection tank 4 with any suitable fixture as would be apparent to one of skill.
FIG. 2 illustrates a second embodiment of the microencapsulation system. System 1' differs from system 1 in that system 1' includes air knife 2' which does not include a plunger mechanism. In air knife 2', needle 14 is coupled to a container 42, which is configured to hold the fluid from which the droplets are to be made, such as alginate solution 7. Container 42 is coupled to a source of pressurized gas (preferably sterilized air) via a feed pipe 44. The pressure of the gas introduced into container 42 is controlled by conventional means to regulate the discharge rate of alginate suspension 7, for example, through needle 14. The discharge rate preferably is regulated to be constant.
Referring to FIGS. 3, 4 and 5, the configuration and position of tubular member or needle 14 relative to sleeve 26 will be discussed. In the preferred embodiment, needle 14 is positioned in sleeve 26, which has an elongated and hollow or tubular shape, and extends beyond outlet opening 30 of sleeve 26. Sleeve 26 can be made of stainless steel or any other suitable sterilizable material. Sleeve 26 terminates at its distal end into a generally blunt edge 31. That is, edge 31 is neither beveled nor sharp.
Needle 14 is an elongated tubular member that is hollow throughout its entire axial length. For the purpose of illustration, the size of needle 14 can range between 16 and 30 gauge, and preferably is 20 gauge. Distal end 32 of needle 14 is beveled at an angle α (the angle formed between side wall 51 of needle 14 and beveled surface 50). Angle α can range from about 15 degrees to 45 degrees to provide the desired results, and preferably is about 22�. As shown in FIG. 3, beveled surface 50 is positioned a short distance below blunt edge 31 of sleeve 26. Preferably the uppermost portion of beveled surface is about 1 mm below blunt edge 31. It should be understood, however, that the present invention contemplates positioning beveled surface 50 at various locations relative to blunt edge 31 of sleeve 26. In addition, although beveled surface 50 is shown facing the air flow path, variations to this position are also contemplated within the scope of the present invention.
Referring to FIG. 5, beveled (elliptical) surface 50 is bounded by an upper edge 60, and side edges 61 and 62 that meet at and terminate into a pointed tip 52. While beveled surface 50 is shown as being flat, it should be understood that beveled surface 50 could alternatively be arcuately shaped to provide an additional contact surface for the droplets to be formed thereon and, thus, help to control the droplet size. The beveled shape of edge 50 represents an important aspect of the present invention, in that the beveled edge allows air knife 2 (or 2') to generate smaller size droplets 34.
As illustrated in FIGS. 3 and 4, needle 14 preferably is eccentrically (i.e., non-coaxially) positioned inside sleeve 26. In other words, center 54 of needle outlet opening 58 is offset from center line (longitudinal central axis) 56 of sleeve 26. This eccentricity also is important to generate small diameter droplets 34. In the preferred embodiment, side wall 51 of needle 14 about the inner wall surface of sleeve 26, so as to prevent air from flowing therebetween.
The eccentric placement of needle 14 further contributes to the regulation of the size of the droplets formed and to the significant reduction of their size. Additionally, the beveled shape of needle 14 and the placement of needle 14 in contact with the inner wall surface of sleeve 26 cause the formation of blank microcapsules of much smaller size than the microcapsules containing cells or tissue. Consequently, the latter microcapsules are readily identifiable and distinguishable from the blank microcapsules, and thus segregable therefrom.
Referring now to FIGS. 6 and 7, there is illustrated an alternate embodiment of air knife 2. This embodiment is similar to the embodiment illustrated in FIGS. 3, 4 and 5, with the single variation that needle 14 is not placed in direct contact with the inner surface of sleeve 26. A spacing 65 is allowed to be formed between needle 14 and sleeve 26, through which pressurized air is allowed to flow.
The size of spacing 65, i.e., the distance between needle 14 and sleeve 26, varies with the desired application of microcapsule generating system 1. Generally, the farther needle 14 is from center axis 56 of sleeve 26 (i.e., the closer needle 14 is to the inner wall of sleeve 26), the fluid extruded through needle 14 tends to accumulate on the back side of beveled surface 50. In contrast to a configuration where the needle 14 is coaxially aligned with, or close to center axis 56 of sleeve 26, greater control to reduce the size of the capsules can be exercised due to increased air flow rate over tip 52 as the needle 14 is moved radially outward.
Turning now to FIGS. 8 and 9, there is illustrated another embodiment of air knife 2. The embodiment is similar to that shown in FIGS. 3, 4 and 5, except that air knife 2 includes a sleeve 70 that is similar in design and construction to sleeve 26, with the exception that sleeve 70 includes a beveled edge 72, rather than blunt edge 31.
The beveled edges 50 and 72 of needle 14 and sleeve 70, respectively, can be rotated relative to each other to attain the optimal desired droplet size and shape. FIG. 8 shows the two beveled edges 50 and 72 having their slopes in the same general direction, while FIG. 9 illustrates the slopes as being oppositely positioned.
The general operation of the device will be described in conjunction with the system illustrated in FIGS. 1 and 3-5 using a particulates suspension in an alginate solution for purposes of example and, thus, is not intended to limit the scope of the invention. Syringe 6 is provided with an alginate suspension and plunger 12 displaced at a constant rate (e.g., 0.1-2 ml/min depending on the size of needle 14 sleeve and the material to be encapsulated). As suspension 7 is discharged from outlet opening 58 in beveled surface 50 of needle 14, pressurized gas (e.g., air) as designated by arrow 29 concurrently is flowed downwardly through a chamber 53 formed between the inner surface of sleeve 26 and the outer surface of needle 14. Some of the gas exiting outlet opening 30 flows toward beveled surface 50 to agitate accumulation 64 of suspension 7 on surface 50. The air currents push the alginate suspension which accumulates at beveled surface 50 of needle 14, toward tip 52, thus forming droplets 34 having a desired shape and size. That is, the gas introduced through feed pipe 28 enters sleeve 26 and exits sleeve 26 via outlet opening 30 whereafter air currents agitate the alginate suspension accumulated on distal end 32 of needle or capillary tube 14 to form very small droplets 34. The droplets are captured in a gelling solution 36, such as calcium chloride solution. In the case where CaCl2 is used, the calcium interacts with the carboxylic acid groups of the alginate to form calcium alginate gel. In general, droplets 34 have a substantially spherical shape. The air flow pressure and plunger speed are regulated so as to generate the desired droplet size and shape as discussed above.
Using the air knife of the present invention, biological material containing microcapsules having diameters from about 20-300 μm are formed. The blanks, microcapsules without biological material, are generally about ten to fiftyfold smaller. Thus, the microcapsules containing biological material are clearly distinguishable from the blank microcapsules based on size and are readily separated as described below.
The very small blank microcapsules are separated from the remaining microcapsules containing the coated biological material, by allowing the latter microcapsules, having a larger size, to settle out, and then by washing away the smaller blank microcapsules. The foregoing process of allowing the larger microcapsules to settle out in tank 4, and the washing away of the smaller blank microcapsules is repeated as many times as needed until the desired concentration of encapsulated islets or other tissue is attained.
Then, the collected encapsulated biological material may be over coated by repeating the process described above. Such over coating will ensure that tissue or cells are completely encapsulated.
The present invention will hereinafter be described more specifically by the following Examples which are provided for illustrative purposes and are not intended to limit the invention. In these examples, a system constructed according to microcapsule generation system 1 was used. The cell suspension in alginate solution was placed in the barrel of the syringe which includes a 20-gauge needle having a pointed tip beveled at a 22� angle. The uppermost edge 60 of beveled surface 50 was positioned about 1 mm below edge 30 of sleeve 26.
A suspension of pancreatic islets in alginate is placed in the syringe barrel. The syringe plunger 12 is displaced to provide a flow rate of 0.3 ml/min to dispense the cell suspension in alginate solution from the needle while air is delivered to outer sleeve 26, which has a 2 mm inner diameter approximately, to provide an entry pressure in sleeve 26 of about 30 psi. Droplets of the suspension fall into collecting vessel 4 containing 120 mM CaCl2 and 10 mM HEPES. The vessel is positioned so that the CaCl2 is about 160-165 mm from tip 52 of the needle. The microcapsules containing pancreatic islets recovered with this procedure had a diameter of about 50 to 300 μm. On the other hand, the blank microcapsules obtained with this procedure had a diameter ranging between 1 μm and 20 μm. Consequently, the blank microcapsules containing islets are readily identifiable and distinguishable from the blank microcapsules for subsequent separation.
An cell suspension in alginate solution comprising rat hepatocytes is prepared. The syringe plunger 12 is displaced to provide a flow rate of 0.3 ml/min. to dispense the suspension from the needle while air is delivered to outer sleeve 26, which has a 2 mm inner diameter approximately, to maintain an internal sheath entry pressure of about 30-33 psi. Droplets of the suspension fall into collecting vessel 4 containing 120 mM CaCl2 and 10 mM HEPES. The vessel is positioned so that the CaCl2 solution is about 160 mm from tip 52 of the needle. Over 90% of the microcapsules recovered with this procedure had a diameter of less than 75 μm. The size distribution is illustrated in FIG. 10 which shows three different preparations of hepatocytes which were run separately according to the procedures described in this example. The consistency of the data from each preparation indicates that these results are reproducible as required for commercial manufacturing.
An alginate suspension comprising Factor VIII secreting cells is prepared. The syringe plunger 12 is displaced to provide a flow rate of 0.3 ml/min to dispense the alginate suspension from the needle while air is delivered to outer sleeve 26, which has a 2 mm inner diameter approximately, to provide a pressure of about 33 psi entering sleeve 26. Droplets of the alginate suspension fall into collecting vessel 4 containing 120 mM CaCl2 and 10 mM HEPES. The vessel is positioned so that the CaCl2 solution is about 154 mm from tip 52 of the needle. 90% of the microcapsules recovered with this procedure had a diameter between 25 and 75 μm. This size distribution is illustrated in FIG. 11 wherein (1) all, (2) pellet, and (3) supernatant correspond to (1) microcapsules containing cells together with blanks, (2) primarily microcapsules containing cells and (3) primarily blanks, respectively.
An suspension of cells secreting calcitonin is prepared in alginate solution. The syringe plunger 12 is displaced at a travel speed of 0.3 ml/min to dispense the suspension from the needle while air is delivered to outer sleeve 26, which has a 2 mm inner diameter approximately, to maintain an internal pressure of about 33 psi entering sleeve 26. Droplets of the suspension fall into collecting vessel 4 containing 120 mM CaCl2 and 10 mM HEPES. The vessel is positioned so that the CaCl2 solution is about 154 mm from tip 52 of the needle. 90% of the microcapsules recovered with this procedure had a diameter between 25 and 75 μm. This size distribution is illustrated in FIG. 12 in which all, pelleted and supernatant have the same meanings as described in conjunction with Example 3 and FIG. 11.
The above is a detailed description of particular embodiments of the invention. It is recognized that departures from the disclosed embodiment may be made within the scope of the invention and that obvious modifications will occur to a person skilled in the art. The full scope of the invention is set out in the claims that follow and their equivalents. Accordingly, the claims and specifications should not be construed to unduly narrow the full scope of protection to which the invention is entitled.
|Cited Patent
|Filing date
|Publication date
|Mar 28, 1979
|Oct 5, 1982
|Damon Corporation
|Encapsulation of biological material
|Nov 13, 1981
|Jun 7, 1983
|Damon Corporation
|Apparatus for producing capsules
|May 1, 1981
|Jul 5, 1983
|Damon Corporation
|Microcapsules containing viable tissue cells
|Apr 28, 1982
|Oct 11, 1983
|Damon Corporation
|Preparation of substances with encapsulated cells
|Feb 13, 1984
|May 5, 1987
|Damon Biotech, Inc.
|Encapsulation of materials
|May 6, 1985
|Jun 23, 1987
|Washington University Technology Associates
|Method for coating particles or liquid droplets
|Apr 30, 1986
|Sep 8, 1987
|Damon Biotech, Inc.
|Method and apparatus for forming droplets and microcapsules
|Jul 16, 1984
|Dec 6, 1988
|Connaught Laboratories Limited
|Microcapsule composition suitable for cardiovascular injection
|Jul 28, 1986
|Jan 24, 1989
|Mitsui Toatsu Chemical, Incorporated
|Process and apparatus for producing immobilized enzyme granules
|Feb 2, 1987
|Mar 21, 1989
|Snow Brand Milk Products Co., Ltd.
|Production process of encapsulated bodies
|Aug 26, 1986
|May 9, 1989
|Hitachi, Ltd.
|Apparatus and process for producing gel beads of microbial cells or enzymes
|Sep 3, 1987
|May 1, 1990
|Massachusetts Institute Of Technology
|System for delayed and pulsed release of biologically active substances
|Nov 13, 1986
|Sep 11, 1990
|Connaught Laboratories Limited
|Droplet generation
|Dec 7, 1988
|Aug 20, 1991
|Snow Brand Milk Products Co., Ltd.
|Apparatus for preparing encapsulated bodies
|Jan 21, 1991
|May 28, 1992
|Gudmund Skjak-Braek
|Homologous guluronic acid alginate coating composition for in-vivo application and implatation and method of using same
|Title not available
|Title not available
|A. C. Hulst, et al., A New Technique for the Production of Immobilized Biocatalyst In Large Quantities, Biotechnology and Bioengineering, vol. XXVII, pp. 870 876, (1985).
|A. C. Hulst, et al., A New Technique for the Production of Immobilized Biocatalyst In Large Quantities, Biotechnology and Bioengineering, vol. XXVII, pp. 870-876, (1985).
|J. Klein, et al., Pore Size and Properties of Spherical Ca Alginate Biocatalyst, European Journal of Applied Microbiology and Biotechnology, 18:86 91 (1983).
|J. Klein, et al., Pore Size and Properties of Spherical Ca-Alginate Biocatalyst, European Journal of Applied Microbiology and Biotechnology, 18:86-91 (1983).
|Citing Patent
|Filing date
|Publication date
|Mar 22, 1999
|Oct 16, 2001
|Duke University
|Method of culturing, cryopreserving and encapsulating pancreatic islet cells
|Dec 1, 1999
|Apr 2, 2002
|Duke University
|Methods of culturing and encapsulating pancreatic islet cells
|Aug 25, 2000
|Apr 16, 2002
|Islet Sheet Medical, Inc.
|Retrievable bioartificial implants having dimensions allowing rapid diffusion of oxygen and rapid biological response to physiological change, processes for their manufacture, and methods for their use
|Jul 25, 1995
|Oct 15, 2002
|Ulrich Zimmermann
|Preparing small microcapsules containing high concentration of cells or tissue
|Jul 5, 2001
|Nov 18, 2003
|Islet Technology, Inc.
|System and method for encapsulating biological material by applying electrostatic charge to capsules
|Jan 23, 2002
|Aug 31, 2004
|Duke University
|Microencapsulated pancreatic islet cells
|Sep 19, 2002
|Apr 11, 2006
|Medtronic, Inc.
|Medical assembly suitable for long-term implantation and method for fabricating the same
|Dec 31, 2002
|Sep 5, 2006
|Area Laboratories, Llc
|Hemostatic compositions and methods for controlling bleeding
|Jul 8, 2011
|Sep 10, 2013
|Cytonome/St, Llc
|Multilayer hydrodynamic sheath flow structure
|May 12, 2011
|Nov 18, 2014
|Empire Technology Development Llc
|Bioreactor for engineered tissue
|Jun 6, 2005
|Oct 13, 2005
|Cochrum Kent C
|Hemostatic polymer useful for RAPID blood coagulation and hemostasis
|Feb 17, 2006
|Jun 29, 2006
|Crosslink-D, Incorporated, A Delaware Corporation
|Hemostatic compositions and methods for controlling bleeding
|Jan 18, 2008
|Jul 24, 2008
|Monika Barbara Horgan
|Composition and method of stabilized sensitive ingredient
|Jan 12, 2012
|Cytonome/St, Llc
|Multilayer Hydrodynamic Sheath Flow Structure
|May 12, 2011
|Nov 15, 2012
|Empire Technology Development Llc
|Bioreactor for engineered tissue
|U.S. Classification
|435/174, 428/402.2, 435/283.1, 435/178, 435/284.1, 264/4.7, 435/177
|International Classification
|Cooperative Classification
|European Classification
|Jan 24, 1994
Owner name: REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE, CALI
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DORIAN, RANDEL E.;COCHRUM, KENT C.;REEL/FRAME:006854/0580;SIGNING DATES FROM 19940120 TO 19940124
|Apr 17, 1995
Owner name: METABOLEX, INC., CALIFORNIA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE;REEL/FRAME:007465/0214
Effective date: 19950327
Owner name: REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE, CALI
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE;REEL/FRAME:007465/0214
Effective date: 19950327
|Apr 1, 1997
|Certificate of correction
|Nov 24, 1999
|Fee payment
Year of fee payment: 4
|Feb 7, 2002
|Nov 26, 2003
|Fee payment
Year of fee payment: 8
|Nov 28, 2007
|Fee payment
Year of fee payment: 12
|Dec 3, 2007
|Maintenance fee reminder mailed


text:
Finishing college for free
Updated: 07/14/2009 - Some Eastern Kentuckians will be eligible to head back to college...for free. A new program is providing a great opportunity for adults who want to take another try at furthering their education.
Updated: 07/14/2009 - Some Eastern Kentuckians will be eligible to head back to college...for free. A new program is providing a great opportunity for adults who want to take another try at furthering their education.
Posted: 07/10/2009 - A federal appeals court throws out a lawsuit filed by a Letcher County miner, calling for stricter limits on coal dust that can lead to black lung.
Posted: 07/10/2009 - here is a boil water advisory for some people in the Bledsoe community of Harlan County.
Posted: 07/10/2009 - State officials are investigating accidents at two Kentucky coal mines that injured ten workers.
Updated: 07/10/2009 - The new facility is expected to house inmates soon.
Updated: 02/05/2010 - U.S. Congressman Harold "Hal" Rogers (KY-05) announced today that a key committee in the U.S. House of Representatives has approved federal funding for flood control efforts in southern and eastern Kentucky.
Posted: 07/09/2009 - Police find more than one hundred marijuana plants in Pike County.
Posted: 07/09/2009 - The sheriff says all the components needed to make a meth lab were found at the home of 30-year old Jason Ray Marcum in McKee.
Posted: 07/09/2009 - CENTRAL CITY, Ky. (AP) - Company officials say eight miners were injured in a mantrip accident at a western Kentucky mine, with a broken leg the most serious injury.
Updated: 07/10/2009 - The publisher of The Courier-Journal says the newspaper has eliminated 44 jobs, or 7 percent of its work force, as part of a broader reduction across Gannett Co. Inc.'s local newspaper division.
Posted: 07/09/2009 - The Pike County Fiscal Court agrees to combine the offices of energy and community development.
Updated: 01/06/2010 - The Paintsville City Council approves an ordinance regulating alcohol sales in the city.
Updated: 07/09/2009 - The bond for a former sheriff's deputy charged in connection with the murder of a former sheriff, will not be reduced.
Updated: 02/05/2010 - There are some encouraging signs in one eastern Kentucky downtown community that is in the process of revitalization. Discover Downtown Middlesboro officials hope it is just the beginning.
Updated: 07/09/2009 - Eastern Kentucky's weather has gone from one extreme to the other this year, causing headaches for allergy sufferers.
Updated: 07/10/2009 - A new report by the Department of Housing and Urban Development shows homelessness trends in America are changing, especially in some rural areas like Eastern Kentucky.
Updated: 07/10/2009 - Police are investigating after explosives were found in Pulaski County neighborhood.
Posted: 07/09/2009 - SPRINGFIELD, Mass. (AP) - Do you use a sock puppet to secretly keep track of your frenemies?
Posted: 07/09/2009 - Officers with the Pulaski County Sheriff's Department say 28-year told Paul Mounce was hit just before noon on highway 1247 at Elihu Cabin Hollow Road by his Fiancé.
Updated: 07/09/2009 - The Pike County Domestic Violence Shelter is temporarily closed due to funding problems.
Updated: 07/10/2009 - A newspaper reporter claims the Clay County Judge Executive tried to choke him in front of the sheriff.
Posted: 07/09/2009 - Police say they found the suspects at the Super 8 Hotel off of the Hal Rogers Parkway with dozens of OxyContin and other pills and marijuana.
Updated: 07/09/2009 - The Martin County Sheriff says that the suspect in Monday's shooting is now in jail.
Posted: 07/09/2009 - U.S. Small Business Administration to create Disaster Loan Outreach Centers for flood victims.
Posted: 07/09/2009 - FRANKFORT, Ky. (AP) - Lexington attorney Gatewood Galbraith has filed a letter of intent with the state saying he intends to run for governor in 2011.


text:
AVE MARIA, FL. The Fire caught flight to the first phase of preseason training on Sunday with one notable absentee.
There could be a thaw coming in the icy relationship between the Cubs and Sammy Sosa.
For his first tattoo, Javier Baez went with the Major League Baseball logo on the back of his neck.
The Hall of Fame shutout only reinforced the belief that everyone from Kerry Woods generation will be under suspicion.
Coincidentally, a day after the Baseball Writers Association of America did not elect a single player into the National Baseball Hall of Fame, Major League Baseball announced a major modification i


text:
0 Your Favorites
Follow Us
Homemade European Groupsex
Black Ebony Ass
Amateur russian threesomes
Indian Xxx Full
Amateur threesomes
Blondes facials threesomes
S xxx little princess
Home porn video
Homemade Threesomes Fmfcumshot
Gang Bang Russian
Groupsex Mmf Threesomes
Gangbang Group Sex
Amateur black and ebony hardcore
Bbw big boobs threesomes
Amateur gangbang threesomes
Sclip Dancingbear Com
Matures threesomes
Threesomes Roleplay
Blondes group sex hardcore
Amateur milfs threesomes
Blowjobs facials teens
Amateur Threesome Mff
Homemade Groupsex European
Anal blowjobs threesomes
Amateur brunettes threesomes
Blondes group sex milfs
Bisexuals threesomes
Groupsex Interacial Ffm
Outdoors Public Groupsex
Cumshots funny threesomes
Homemade Teenager First
Homemade Couple Webcam
Group sex sex toys bisexuals
Granny Old Teenager
brazil xxx
Prone Bone Riding
Classic Retro Ass
Anal teens threesomes
Homemade Teenager
Brunettes group sex lesbians
Babes close-ups pov
Babes lesbians threesomes
Redhead Threesome Blonde
Homemade Couple Groupsex
Double penetration interracial threesomes
Amateur cumshots nipples
Amateur Teen Threesome
Hardcore teens threesomes
Amateur group sex hardcore
Homemade Interacial Threesomes
Ffm Threesome
Big boobs blondes threesomes
Threesomes 3some Sucking
Lesbians strapon threesomes
Milfs old + young threesomes
Homemade Threesomes Ffm
Group sex pornstars vintage
Homemade Webcam Couple
Threesomes Ffm
Blondes threesomes tits
French old + young threesomes
Teenager Outdoors Public
Blowjobs hardcore threesomes
Amateur Homemade Beach
Sclip Wifebucket Com
Facials hardcore threesomes
Anal cumshots public nudity
German matures threesomes
Teenager Groupsex Teens
Hardcore threesomes
Brunettes pornstars vintage
Anal brunettes group sex
Homemade Groupsex Threesomes
Ffm Threesomes Lesbian
Groupsex Threesome Reality
Celebrities lesbians threesomes
Cumshots hardcore threesomes
Amateur lesbians teens
(C) VideoSexArchive.com | DMCA | 2257 | Privacy | Terms | Contact
VideoSexArchive is rated with RTA label. Parents, you can easily block access to this site. Please read this page for more informations.
VideoSexArchive is a free hosting service for your porn videos. Our pages contain absolutely no spyware/adware/trojan/etc. There is no charge (no hidden charges either) for viewing our videos.