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Head Trader Of World's 4th Largest Hedge Fund Caught In HFT Frontrunning ScandalSubmitted by Tyler Durden on 07/31/2015 10:25 -0400
Shortly after we reported the latest market-rigging scandal, in which ITG was busted for frontrunning sellside clients in its dark pool in what has been since dubbed a "trading experiment" (because it sounds better than criminal conspiracy to defraud clients), and which will cost the company a record for a private Wall Street firm $22 million settlement, we had one question for AQR's Cliff Asness yesterday morning: "Hi @Cimmerian999, is Hitesh Mittal the AQR employee who was formerly at ITG and is part of the SEC settlement?" We got no answer from the AQR head, but luckily Bloomberg noticed, and as it turns out the answer to our question was a resounding yes.
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Bail-Ins at "Bad Bank" Unconstitutional Says Austrian CourtSubmitted by GoldCore on 07/29/2015 13:39 -0400
The ruling does not outlaw "bail-ins" per se. It simply ensures that guarantees given to bondholders cannot be retrospectively revoked. EU nations who have not yet ratified the BRRD have until the end of July to adopt the new EU bail-ins rules
- GoldCore's blog
- 8 comments
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- 9642 reads
Supply And Demand In The Gold And Silver Futures MarketsSubmitted by Tyler Durden on 07/28/2015 23:15 -0400
The bear market in bullion is an artificial creation. This artificial, indeed fraudulent, increase in the supply of paper bullion contracts drives down the price in the futures market despite high demand for bullion in the physical market and constrained supply.
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- 85138 reads
Gold "Extremely Rare" - All World's Gold Fits In Average Four-Bedroom HouseSubmitted by GoldCore on 07/27/2015 07:02 -0400
Some downward risk to the gold price remains due to the momentum of the recent severe correction in price. He points out that GoldCore had suggested on Bloomberg three years ago that a 50% correction in price was not unlikely at that time as is normal in long term bull markets.
- GoldCore's blog
- 103 comments
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- 32193 reads
Can You Hear the Fat Lady Singing? - Part IIISubmitted by Capitalist Exploits on 07/26/2015 22:08 -0400
The unwinding of the US dollar carry trade will be particularly severely felt where leverage is highest!
- Capitalist Exploits's blog
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- 24394 reads
It's Really Very Simple...Submitted by Tyler Durden on 07/26/2015 21:10 -0400
There are times when a loud cry of "The emperor has no clothes!" can be most copacetic. And so, let me point out something quite simple, yet very important. The old world order, to which we became accustomed over the course of the 1990s and the 2000s, its crises and its problems detailed in numerous authoritative publications on both sides of the Atlantic - it is no more. It is not out sick and it is not on vacation. It is deceased. It has passed on, gone to meet its maker, bought the farm, kicked the bucket and joined the crowd invisible. It is an ex-world order.
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Central Banks Ready To Panic - AgainSubmitted by Tyler Durden on 07/26/2015 15:00 -0400
Less than a decade after a housing/derivatives bubble nearly wiped out the global financial system, a new and much bigger commodities/derivatives bubble is threatening to finish the job. So... the central banks will panic. Again. Countries that retain some control over their monetary systems will see their interest rates fall to zero and beyond, while those that don't will be thrown into some kind of new age hyperinflationary depression. Not 2008 all over again; this is something much stranger.
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- 49192 reads
The Eurasian Big Bang: How China & Russia Are Running Rings Around WashingtonSubmitted by Tyler Durden on 07/24/2015 21:15 -0400
Tehran, Beijing, Moscow, Islamabad, and New Delhi have been actively establishing interlocking security guarantees. They have been simultaneously calling the Atlanticist bluff when it comes to the endless drumbeat of attention given to the flimsy meme of Iran's "nuclear weapons program." And a few days before the Vienna nuclear negotiations finally culminated in an agreement, all of this came together at a twin BRICS/SCO summit in Ufa, Russia -- a place you've undoubtedly never heard of and a meeting that got next to no attention in the U.S. And yet sooner or later, these developments will ensure that the War Party in Washington and assorted neocons (as well as neoliberalcons) already breathing hard over the Iran deal will sweat bullets as their narratives about how the world works crumble.
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- 45973 reads
Gold Smash Leads to Surge in Demand For Coins, Bars Around WorldSubmitted by GoldCore on 07/23/2015 06:12 -0400
The manipulative smash on the gold price on Sunday night has once again led to a surge of buying of gold coins and bars across the globe. Both the Wall Street Journal and Reuters report on how bullion dealers are seeing a spike in demand for gold coins and bars in India and China and indeed Europe, Australia and the U.S.
- GoldCore's blog
- 65 comments
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- 32452 reads
Nine Reasons Why Low Oil Prices May "Morph" Into Something Much WorseSubmitted by Tyler Durden on 07/22/2015 14:20 -0400
Why are commodity prices, including oil prices, lagging? Ultimately, it comes back to the question, "Why isn't the world economy making very many of the end products that use these commodities?" If workers were getting rich enough to buy new homes and cars, demand for these products would be raising the prices of commodities used to build and operate cars, including the price of oil. If governments were rich enough to build an increasing number of roads and more public housing, there would be demand for the commodities used to build roads and public housing. It looks to me as though we are heading into a deflationary depression, because prices of commodities are falling below the cost of extraction. We need rapidly rising wages and debt if commodity prices are to rise back to 2011 levels or higher. This isn't happening.
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Trumpism: The IdeologySubmitted by Tyler Durden on 07/18/2015 19:45 -0400
It's not too interesting to say that Donald Trump is a nationalist and aspiring despot who is manipulating bourgeois resentment, nativism, and ignorance to feed his power lust. It's uninteresting because it is obviously true. It's so true that stating it sounds more like an observation than a criticism. However, 'Trumpism' is an ideology that is very much present in American life, though hardly acknowledged. It lives mostly hidden in dark corners, and we don't even have a name for it. You bump into it at neighborhood barbecues, at Thanksgiving dinner when Uncle Harry has the floor, at the hardware store when two old friends in line to checkout mutter about the state of the country. The ideology is a 21st century version of right fascism - one of the most politically successful ideological strains of 20th century politics. Though hardly anyone talks about it today, we really should.
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Putin Orders Formation Of New Military Reserve ForceSubmitted by Tyler Durden on 07/18/2015 17:30 -0400
Fifteen years after Vladimir Putin first walked into the Kremlin, Russia's army is bigger, stronger, and better equipped than at any time since the end of the Cold War. Able to call on three quarters of a million frontline troops, The Telegraph reports, with more tanks than any other country on the planet, and the world's third largest air force, Russia retains much of the brute force associated with a former superpower. But it has also rapidly modernised, spending millions on rearmament and retraining programmes aimed at professionalising the lumbering, conscript-reliant force it inherited from the Soviet Union. The latest effort, as Reuters reports, Putin has ordered the creation of a new reserve armed force as part of steps to improve training and military readiness at a time of international tensions with the West over Ukraine.
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Have Central Banks Brought Us Back to 2008… or 1929?Submitted by Phoenix Capital Research on 07/18/2015 12:34 -0400
The last time these criteria were met... stocks plunged over 90% over the next 24 months.
- Phoenix Capital Research's blog
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- 17817 reads
Donald Trump The DemagogueSubmitted by Tyler Durden on 07/17/2015 21:15 -0400
One of the reasons we write is rooted in our deep fear of what might emerge after the current paradigm collapses. We have no doubt something very different is coming, we just desperately want that thing to be freedom, free markets and prosperity as opposed to the disaster that a $2 despot like Trump would bring. His ascension in the polls is very troubling, and makes us wonder whether the public will ultimately choose to rally behind some statist-demagogue wrapped in an American flag when things get bad enough, as opposed to something far more difficult: Liberty. We fear they may eventually choose someone like Donald Trump.
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China Increases Gold Holdings By 57% "In One Month" In First Official Update Since 2009Submitted by Tyler Durden on 07/17/2015 14:36 -0400
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[Video] 2012 DJ Expo - American Audio Booth
We're here at the Atlantic City DJ Expo and we've got loads of HD-Video coverage to show you all, so keep it locked for the next 24-hours! First up, we have the American Audio Booth at the Expo where they had all of their DJ controllers and lots more on full display for everyone to see, use, and purchase. Check out our HD-Video of the entire American Audio booth to see how it's all still going down as we speak!
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There's a nifty new tool for Twitter called Sulia that's doing a good job organizing feeds from Egypt, and weeding out the useless info. You can follow it by clicking right here.
- Previous Jeffrey Goldberg Post Hillary Clinton: Mubarak Regime is 'Stable'
- Next Jeffrey Goldberg Post A Complete Crackdown in Cairo?


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Shanette has an extensive background ranging from radio host, to consultant, and writer. Featured in magazines for her mixtapes and on air skills, she hosted many radio show formats such as hip-hop, and jazz. By age 18, she interviewed recording artists such as CeeLo Green, Twista, Trey Songz, Chris Brown. During undergraduate studies at Virginia State University, she tested her networking skills and met Sean "P.Diddy" Combs., Lupe Fiasco, and DJ Kid Capri. By age 19, she interned for major record labels such as Jive Records, Def Jam Recordings and Interscope/Universal.
Today, Shanette is helping rising career professionals and career changers market their skills and find their niche in the workforce. Learn "How to Operate with Purpose" is her latest entry into her express edition self-help audio guide. Buy now on Amazon and iTunes.
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We'll hear the first of a two-part story from the CBS Radio Workshop this week. From February 17, 1957, here is The Space Merchants.
We hear from The Whistler this week with their very first episode. Airing May 16, 1942, here is Retribution.
We hear again from The Lives Of Harry Lime on today's show. The Blue Caribou first aired July 18, 1952. This is the second to last episode of the series.
This episode of The Great Gildersleeve is titled, Gildy's Rival, Dr. Olsen. It first aired October 26, 1949.
Lights Out was first broadcast in 1934, and last broadcast in the summer of 1947. The series ran on various networks between those years, sometimes being brought back as a short group of revival shows. From that series we'll hear Until Dead, which first aired February 2, 1943.
The Chase aired over NBC stations for 55 episodes between April 1952, and June 1953. From it we'll hear The Newspaper Reporter, which first aired May 25, 1952.
This week, Frontier Town presents their episode from May 20, 1952 titled, Todd Ford.
On this episode of Legacy Of Laughs we hear from The Milton Berle Show. From March 2, 1948 here is Salute To Literature.
A tale from The Hall Of Fantasy on this Wednesday's show. We'll hear The Wild Huntsman, an episode first heard January 29, 1951.
This week on Case Closed we begin with Night Watch. We'll hear Fence 447 And Western Union Raid from September 4, 1954. Then, Five Minute Mystery brings us, The Needle. Lastly, a little Sherlock Holmes with this episode from 1946 titled, The Demon Barber.


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Taupo Motorsport Park - Home of New Zealand Motorsport
Taupo Motorsport Park is New Zealand's Premier motorsport and driver training facility. As an international FIA category 2 race circuit and NZDRA certified drag strip, we play host to top world class motorsport events making Taupo the "Home of New Zealand Motorsport".
As a venue for conferences, corporate functions, weddings, driver training, race experience, product launches and a specialist motor industry business centre, Taupo Motorsport Park brings a Total Driving Experience to New Zealand.
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Kukla's Korner Hockey
Report: Michael Nylander Signed by Edmonton Oilers
by Alanah McGinley on 07/02/07 at 05:14 PM ET
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via TSN,
According to the Team 1260 in Edmonton, the Oilers have signed veteran centre Michael Nylander.
The report did not include any contractual details.
more later…
Update 3:14pm ET:
Japer's Rink has stated that 630 CHED radio in Edmonton "is now reporting that Nylander has agreed to 4 years with Edmonton. Salary was not disclosed."
Nothing from the Oilers, just this earlier comment from Allan Watt, the Oilers VP of Communications, who is quoted as saying "The deal is done when [GM] Kevin Lowe tells me it's done."
Update: 4:54pm ET:
Capitals have signed Nylander. More here.
Note: Post originally published at 1:39am ET, July 2.
Filed in: Edmonton Oilers, | KK Hockey | Permalink
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DESERT Dead Sea Rift Transect: An interdisciplinary research project to study the Dead Sea Transform
ABSTRACT Since the advent of plate-tectonics the Dead Sea Transform (DST) has been considered a prime site to examine geodynamic processes. It has accommodated a total of 105 km of left-lateral transform motion between the African and Arabian plates since early Miocene (~20 My). Large historical earthquakes on the DST with magnitudes up to 7 and the 1995 Nueiba M7.2 event, as well as ongoing micro-seismic activity show that the DST is a seismically active plate boundary. The DST therefore poses a considerable seismic hazard to Palestine, Israel, and Jordan. The DST segment between the Dead Sea and the Red Sea, called Arava/Araba Fault (AF), is studied in DESERT in detail, using a multi-disciplinary and multi-scale approach from the micrometer to the plate-tectonic scale. This volume contains the results of the DESERT project running from 2000 to 2006. It opens with a review paper followed by 33 special papers. Available at http://dx.doi.org/10.2312/GFZ.b103-09084 (open access).
- References (63)
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- SourceAvailable from: Nils Maercklin[Show abstract] [Hide abstract]
ABSTRACT: The Dead Sea Rift Transect (DESERT 2000) is a multinational and interdisciplinary study of the Dead Sea Rift. The project began field work in February 2000 and the first experiments were successfully completed in May. The seismic, seismological, and magnetotelluric experiments presented here, along with the future electromagnetic, gravity, magnetic, geodynamic, and geological studies, will provide the basic geophysical frame for further geoscientific research. DESERT 2000 should also help to address a fundamental question of plate tectonics: How do shear zones work and what controls them?Eos Transactions American Geophysical Union 12/2000; 81(50):609. DOI:10.1029/EO081i050p00609-01
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ABSTRACT: The Dead Sea Transform (DST) is a major plate boundary separating the African and Arabian plates. It extends over 1000 km from the Red Sea rift in the south to the Taurus collision zone in the north. Present-day left-lateral motion is 4±2 mm/year which is consistent with the kinematics of the Arabian plate assuming a rotation rate of about 0.4°/Ma around a pole at 31.1°N and 26.7°E relative to Africa. The DST became active about 18-21 Ma ago and since then, it has accommodated about 100 km of left-lateral slip. In the area between the Dead Sea and Red Sea the DST is marked by the Arava fault which may have the potential to produce Mw ~ 7 earthquakes along some of its segments about every 200 years. The aim of the interdisciplinary and multi-scale Dead Sea Rift Transect (DESERT) project is to shed light on the question of how large shear zones work. DESERT consists of several geophysical sub-projects that are carried out by partners in Germany, Israel, Jordan and Palestine. Principal investigators are Michael Weber in Germany, Zvi Ben-Avraham in Israel, Khalil Abu-Ayyash in Jordan, and Radwan El-Kelani in the Palestine Territories. One of the sub-projects was a large-scale passive seismic experiment which was conducted in Israel, Jordan, and the territory of the Palestinian Authority. Aims of the project are (a) study of crust and mantle structure with the receiver function (RF) method, (b) travel-time tomography, (c) to investigate azimuthal anisotropy in crust and upper mantle from shear wave splitting, and (d) the study of local seismicity. In this note, we give a brief overview on the field experiment and the data archiving procedure.
Article: Boundary-layer mantle flow under the Dead Sea Transform fault inferred from seismic anisotropy[Show abstract] [Hide abstract]
ABSTRACT: Lithospheric-scale transform faults play an important role in the dynamics of global plate motion. Near-surface deformation fields for such faults are relatively well documented by satellite geodesy, strain measurements and earthquake source studies, and deeper crustal structure has been imaged by seismic profiling. Relatively little is known, however, about deformation taking place in the subcrustal lithosphere--that is, the width and depth of the region associated with the deformation, the transition between deformed and undeformed lithosphere and the interaction between lithospheric and asthenospheric mantle flow at the plate boundary. Here we present evidence for a narrow, approximately 20-km-wide, subcrustal anisotropic zone of fault-parallel mineral alignment beneath the Dead Sea transform, obtained from an inversion of shear-wave splitting observations along a dense receiver profile. The geometry of this zone and the contrast between distinct anisotropic domains suggest subhorizontal mantle flowNature 11/2003; 425(6957):497-501. DOI:10.1038/nature01982 · 41.46 Impact Factor


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Woofhoof TRTS Reward Pouch with Dog Bone Motif
The TRTS Reward Pouch is a creative accessory designed to hold the positive rewards for your horse and dog to help reinforce his best efforts and successful behavior.
The TRTSReward Pouch is lightweight and stylish and attaches to your hip for "at your fingertips" access to treat rewards. The pouch's outer layer is made of durable Cordura, used in backpacks and mountaineering gear and its inner layer is a rugged nylon, used to make tents and other outdoor products. The pouch is finished with customer embroidery, attractive piping and a magnet closure for your ultimate convenience.
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More Labrador Retriever Puppies and Dogs for sale.
4XGMPR Sired Pointing Lab Pups Spring Creek Kennels in Newtown, Missouri
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SOLD Beautiful Yellow Lab Puppies~EIC/CNM CLEAR~Males & Females Ready 21-28 - Labrador Retrievers in Muldrow, Oklahoma - Ark Pilot Labradors
Yellow Lab Puppies Available 2 Males and 3 Females Whelped 10 October 2013. They have already been Dew Clawed and will have a Well Puppy Exam, Properly Wormed, and have their 1st Puppy Shots at the time of Home Placement. They will be Field Prepped and Ready to go Thanksgiving Week. Excellent prospects for Hunting, Field Trials, Hunt Tests, and Loving, Home Companions. You will find pertinent information on my webpage to make a knowledgeable decision to purchase a Healthy, Well-Bred Puppy that has had Proper Socialization and Excellent Care. They are AKC Registered and you will receive Full-Registration Privileges. Make sure your puppy is EIC/CNM CLEAR. We take pride in breeding healthy puppies that are EIC/CNM CLEAR http://d2bm3ljpacyxu8.cloudfront.net/width/300/crop/0,0,300x225/arkpilotlabs.webs.com/IMG_1882.JPG
Ad location: Muldrow, Oklahoma
Ad created: Oct 20, 2013
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I have a C# WinForms application that imports sales contained in a fixed width file and stores it into some tables. The tables have the exact same structure but are located in different schemas inside the database.
Currently I run two queries in my application to store each sale's data, one to fetch the correct schema to store the data and another to actually insert it into the table.
To better exemplify, let's say I have a Clients table in a Main schema and several schemas (one for each region of my country) with a Sales table each. I use each sale's Client Id to find the correct schema to store it.
I need to optimize this proccess, make it faster. I profiled the application and this two queries are the most time consuming steps. I think running a single command inside my application and leaving the work of finding the correct schema to the database will help.
I want to achieve something like this:
(SELECT clients.schema_name FROM main.clients WHERE client_id = @client_id).sales
(client_id, qty, value)
(@client_id, @qty, @value)
Clearly it doesn't works. I don't know much about SQL, just the basics, but I think that's not possible to do with in a single command, I guess I need a Stored Procedure for that.
I would love to hear some directions or even better solutions for this (as long it does not involves changing the database structure).


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H! by Henry Holland Designer yellow bow pinafore dress
Item No.0370108323
Item No.0370108323
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Henry Holland brings you this yellow pinafore dress with a repeated bow pattern, exclusively from his designer H! collection. It has crossed over straps with large button fastenings and a fitted bodice with a shirred back panel.
UK Home Delivery Free (UK orders over £30) or £3.49
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With that infectious smile and enviable physique, Franky Cammarata could sell snow to an Eskimo. Hell, he could probably convince them to purchase a pair of skimpy swim trunks as well. Ergo this fashion spread.
The Orange County native stars in a swimwear editorial for Collezioni Uomo magazine. Photographer Rick Day snaps the 6'2″ stud wearing a wide range of styles from Charlie by Matthew Zink, Lacoste, Onia and other labels.
Check out more pics below. It's almost not fair that someone could look this good. Fingers crossed, he's dumb as rock ;)
First and foremost, huge thanks to Daniel Garofali for sending these photos our way. Much appreciated. The stunningly gorgeous stud graces the cover of DNA's Swimsuit Issue. Those lucky Aussies are enjoying Summer while poor us in North America are in the midst of Winter. BRRRRRR!!!
Photographer Rick Day captures Garofali's masculine beauty as he wears an assortment of bathing suits, ranging from modest to very revealing. Let's just say you can almost see the pot of gold at the end of his treasure trail in certain shots. Check out Daniel below writhe and splash around on the beach for the Aussie publication. It's the perfect way to warm up this cold December day.
This post is too hot to handle for any other day of the week than Saturday. Since most of you are probably reading this while at home rather than the office, you're in the clear to check out the slightly NSFW images. Using photos from his best-selling photo book All Players, famed photographer Rick Day picked a dozen of them for a 2014 calendar. Check out a few of the sizzling visuals below.
ES Collection has Kirill Dowidoff. Not to be outdone. C-IN2 underwear has an equally studly underwear model of their own in the form of Mike Stalker. I sense a future Who'd You Rather battle between them soon. Prior to showing off his juicy bubble butt and rock hard abs, Stalker was a former Fordham University soccer player. That explains that toned physique.
For their latest promo vid, C-IN2 puts that physique to good use. The clip follows Mike during his morning routine which includes a sweaty workout and hammering??? Symbolism much? The vid takes an unexpected turn when a female guest arrives. Before you know it, Mike gets all up on her (lucky bitch). But wait… out of nowhere another person comes in and it's another guy. Did someone call for a threesome. This just got interesting!!! Check out the Rick Day-directed visual below. In my next life, I want to come back as Day. That guy gets to photograph all the hottest guys.
Looking at these images made me reminisce over Brian and my trip to Italy a couple years back. One of the things I regret most from my time it Italia was not venturing to Tuscany and hitting up Florence. Instead, we headed over to Venice for one of our day trips from Rome. It was a bit underwhelming to say the least. That said, I'm sure I wouldn't have seen guys as hot as Fabio Mancini cavorting around in their underwear in the Tuscan countryside had we taken the train to Florence- or as they, Firenze.
The Italian model is front and center of C-IN2 clothing's latest ad campaign. Snapped by famed photographer Rick Day, Mancini smolders in the brand's wide range of underwear styles using the beautiful landscape of Italy as a gorgeous backdrop. Check out Fabio below in numerous photos as he shows off new colors and styles from C-IN2, including Pop, Prime, Zen, Core and Grip.
Todd Sanfield, whom we've featured on Homorazzi a number of times, was featured in last month's issue of GT Magazine for an incredibly sexy editorial shoot shot by the talented Rick Day. In this post, we've included some of those photos as well as some of the outtakes that didn't make it into the issue.
The shoot took place in Kew Motel in Queens, the type of place that offers "very reasonable short-stay rates" (if you know what I mean), as well as as standard overnight rates. Todd poses in various parts of the motel room wearing different pairs of underwear, and sometimes nothing. There are some photos that are a little too racy for our site, but let me tell (as if I haven't said it before) Sanfield has the hottest…everything. He's got an incredible chest, killer abs, a bootylicous butt, and well…I'll just stop there before I get carried away.
Check out the incredibly sexy pictures from the shoot below.
With the end of Movember in sight, I thought posting this sexy fashion editorial was very appropriate. Three of today's hottest male models posed for famed photographer Rick Day for a photo series titled "Stiff Upper Lip". The pictures can be found in the premier issue of ADON magazine– a new fashion pop culture magazine from New York.
Daniel Garofali, Travis Hanson, and Brian Shiamansky don fake mustaches for the shoot. Even though you can't see much of the clothing, it's from German brand Matistache. The underwear Garofali is wearing in the above picture has an adorably cute mustache silhouette printed on the behind. I totally want a pair. Check out a couple more pictures from Day's series below.
A new video that launched today, "Leaves Changing, Bodies Blooming," introduces viewers to C-IN2 Clothing Co's new selection of colors for its Fall/Winter 2012 Collection that stays colorful and vibrant. The video was shot on location in Tuscany by fashion photography sensation Rick Day.
"The trend with Fall/Winter color palettes is typically to go darker and more serious. We know that C-IN2's customers like to stay colorful and have our great underwear accent their wardrobe," said C-IN2 founder Gregory Sovell. "It's the age of jet travel, and somewhere in the world, the flowers are always in bloom. As Isabella Rossellini says in Death Becomes Her, 'I follow the spring. I haven't seen an autumn or winter for years.' That's what shapes Fall/Winter 2012 at C-IN2."
The Fall/Winter 2012 features new fall & winter inspired colors for each 7 of C-IN2's groundbreaking lines of underwear: ZEN, now in Plum Wine, True Blue, Dark Emerald, Polar Rose, and Lemoncello, is a combination of Rayon (made from Bamboo) and Cotton fibers blended to give a special transmission of comfort. An update for 2012 replaces the leg bindings with microfiber elastics. The elastic leg tension is adjusted accordingly for the different styles. Check out the stunning photos from the collection as well as the sexy video below. In case you're wondering, the awesome song playing in the video is "I Follow Rivers" by Wounded Rhymes. Enjoy!


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Photo Flash: Hugh Jackman in New WOLVERINE Photo
The X-Men spinoff, THE WOLVERINE is directed by James Mangold and stars Hugh Jackman, Will Yun Lee, Svetlana Khodchenkova, Hiroyuki Sanada, Hal Yamanouchi, Tao Okamoto, Rila Fukushima and Brian Tee.
Based on Chris Claremont and Frank Miller's 1982 Wolverine miniseries, the film follows Logan's troubled adventures in modern-day Japan. There he is forced to confront not only a deadly enemy, but the terrible truth of his own mortality.
Hugh Jackman in THE WOLVERINE
About Author
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The Hymn of Praise is the psalm par excellence—the celebration of God his mighty works and incomparable character. Though not the most numerous of the psalm types, these hymns so color the impact of the Psalter that they gave the collection its name in the Hebrew tradition, sēp̱er tehillîm, "Book of Praise Songs."


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First class hotel physically. Great location in downtown Coral Gables.
Staff accommodating and professional. Perfect English too. Don't take that for granted in Miami.
Rooms nice. I was a happy camper and would return.
- Official Description (provided by the hotel):
- Nestled in the "Beverly Hills of Miami", Hyatt Regency Coral Gables, a stunning AAA Four Diamond hotel set in the heart of Coral Gables offers the unparalleled personalized service and luxurious accommodations that you seek for your weekend getaway. Relax by the pool with a fresh Mojito or enjoy regional flavors at Two Sisters, our award winning restaurant. ... more less
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Date — 30 May, 2012
Abu Dhabi Ocean Racing took a battering by tropical storms before opening up an unassailable lead in Leg 7.
Azzam has put in a determined display to lead the Volvo Ocean Race Leg 7 to Lisbon
Atlantic Ocean, 28 May 2012: A resurgent Abu Dhabi Ocean Racing – the Abu Dhabi-backed entry in the nine-month Volvo Ocean Race – has powered to the 3,590 nautical mile trans-Atlantic Leg 7 lead, after a number of deft tactical decisions saw the team outmanoeuvre the five-strong chasing pack.
Having set off in style from Miami, USA last weekend, the fleet sailed straight into the Gulf Stream and a tropical depression, before being forced to head north to dodge a high pressure system blocking the direct path towards Lisbon. In a leg of two distinct halves, it was Abu Dhabi Ocean Racing which attained maximum gain.
Speaking from onboard the team's Volvo Open 70 race yacht, Azzam, which means determination in Arabic, skipper Ian Walker said their more than 85 nautical mile lead was down to smart thinking by navigator Jules Salter who picked the perfect path through shifting breezes and swirling Atlantic currents to a low pressure system that packs the power to catapult them to within a couple of hundred miles of the finish.
"It's been a great sailing for Azzam. A big dilemma between sailing for the predicted right hand shift and going left to get in the Gulf Stream created a big split in the fleet and lots of opportunity. This is exactly what we need as we can get away from the other boats and try to find something tactically. Jules did a nice job as we initially tried to play the current but on finding it wasn't as forecasted we decided to take the shift instead," said Walker, who led the team to its third In-Port Race win last week in Miami, sending Azzam to joint first in the overall in-port standings.
Currently halfway through the leg to Lisbon, Portugal, Abu Dhabi Ocean Racing has encountered the full spectrum of weather so far, from tropical storms in the opening stages to yacht-halting wind holes, which the team has just navigated through.
"We have just escaped from the fickle winds of the high pressure area and we are once again making good progress east north east along our planned route. It has been a strange 24 hours. It was a period where we thought we stood to make reasonable mileage gains on our opponents but were held frustratingly pinned by light winds as the high pressure centre moved further east and therefore closer to us than envisaged. The wind kept building and offering promise before dying away as fast as it had arrived. At times we were becalmed and going backwards in the current. We cannot be greedy as the important thing is that we have negotiated a very tricky part of the race course and we are still leading. It has been another stellar night's sailing with clear skies and a moon that grows more prominent every night. It is chilly enough for thermals and hats but not for gloves just yet. Ahead of us now lies a lot of running in medium winds which means the mileage to Lisbon should once again start to tick down fast," added Walker.
Abu Dhabi Ocean Racing's watch-leader, and Volvo Ocean Race winner, Rob Greenhalgh, said the team was cautiously optimistic and that the final run in will be critical.
"There is a lot of runway ahead of us still, so it's vital that we continue to sail smart, not just fast, and remember what's waiting for us just off Portugal. The next four days should be a good test for all of us both mentally and physically. We're just happy to be upfront mixing things up a bit. North Atlantic weather systems are never easy, and this particular crossing is proving just how many elements are at work out here," said the 2005/06 race winner.
The six-strong fleet is expected in Lisbon on May 31st 2012, where it will break for 10 days before heading on to L'Orient, France then to the finish in Galway, Ireland in early July.
Sotheby's Geneva and Swiss luxury watch manufacturer IWC Schaffhausen are proud to offer a stunning unique piece, which will be auctioned during Sotheby's sale of Important Watches on 10 November 2015 in Geneva. Read Article
With a watchmaking complication in the form of a large date and day display, the Portofino Hand-Wound Day & Date is an extremely practical timepiece. Read Article
In 2014 luxury Swiss watchmaker IWC Schaffhausen extended the Portofino watch family with a number of models in 37-millimetre cases, making the ever-popular line more attractive to watch lovers who prefer a timepiece with slightly smaller dimensions. Read Article
IWC Schaffhausen has travelled to Uganda to celebratethe Ingenieur Automatic Edition "Laureus Sports for Good Foundation" (Ref. IW323909) featuring an engraving on its back based on a drawing done by thirteen-year-old Nakayenga Zahara. Read Article


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Learn to Create Your New American Dream#1 NEW YORK TIMES BESTSELLER Revised & updatedWHAT WILL YOU LEARN IN THE MONEY CLASS? How to find the courage to stand in your truth and why it is a place of power. What daily actions will restore the word "hope" to your vocabulary. Everything you need to know about taking care of your family, your home, your career, and planning for retirement—no matter where you are in your life or where
$9.99Times have changed and the rules have changed, but financial security is still the goal. Do you know how to get there? There is a new reality out there—a new normal. What was once certain—that you would be able to retire comfortably, that you would pay for your kids' education, that your home would appreciate in value—is no longer a sure thing. So much has changed on the financial landscape that
Owning the Power to Control Your DestinyWhy is it that women, who are so competent in all other areas of their lives, cannot find the same competence when it comes to matters of money?Suze Orman investigates the complicated, dysfunctional relationship women have with money in this groundbreaking new book. With her signature mix of insight, compassion, and soul-deep recognition, she equips women with the financial knowledge and emotional
Suze Orman's Financial GuidebookA One-on-One Financial Planning Session with Suze Orman.With her national bestseller The 9 Steps to Financial Freedom, Suze Orman launched a personal finance revolution—transforming the concept of money for the millions of people across the world who have embraced her message of understanding the psychology involved in our relationship with money. Now, with Suze Orman's Financial Guidebook, you
The Money Book for the Young, Fabulous & BrokeBe sure to catch Suze Orman's latest PBS special based on The Money Book for the Young, Fabulous & Broke, which will air the weekend of March 4th on stations across the country. Check your local listings for airtimes.The Money Book for the Young, Fabulous & Broke is financial expert Suze Orman's answer to a generation's cry for help. They're called "Generation Debt" and "Generation Broke" by the
The Laws of Money, The Lessons of Life
Keep What You Have And Create What You DeserveUSA Today has called Suze Orman "a force in the world of personal finance." For years, Suze has anticipated what you need to know and want to know about your money. Her books, radio and television shows, columns, and newsletter about personal finance have helped millions of people like you turn their financial lives around. The author of three consecutive runaway New York Times bestsellers, Suze
The Prize Winner of Defiance, Ohio
How My Mother Raised 10 Kids on 25 Words or LessThe Prize Winner of Defiance, Ohio introduces Evelyn Ryan, an enterprising woman who kept poverty at bay with wit, poetry, and perfect prose during the "contest era" of the 1950s and 1960s. Stepping back into a time when fledgling advertising agencies were active partners with consumers, and everyday people saw possibility in every coupon, Terry Ryan tells how her mother kept the family afloat by
The 9 Steps to Financial Freedom
Practical and Spiritual Steps So You Can Stop WorryingSuze Orman has transformed the concept of personal finance for millions by teaching us how to gain control of our money -- so that money does not control us. She goes beyond the nuts and bolts of managing money to explore the psychological, even spiritual power money has in our lives. The 9 Steps to Financial Freedom is the first personal finance book that gives you not only the knowledge of how
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Kim Klein's Fundraising Series (Book #31)This newest edition of the classic book shows how anyone can align and integrate values, passions, and dreams for their communities and families into their plans. Inspired Philanthropy explains how to make a difference by creating giving and legacy plans, tells what questions to ask nonprofits, and spells out how to help partner with advisors and nonprofit leaders for inspired outcomes. In
$2.99Wish you could find a money book that doesnt make your eyes glaze over? Easy Money is for you. Gail knows you work hard for your money, so in her usual honest and practical style she will show you how to make your money work for you. Budgeting, saving, and getting your debt paid off have never been so easy to understand or to do. Follow Gails plan and take control of your money. This book is a


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12:00 PM - 1:30 PM Lunch & Learn -Special Education in Port Washington
4:30 PM - 6:00 PM Creative Readers Volunteer Training
Let's Move! Active Schools, a sub-initiative of first lady Michelle Obama's Let's Move! program, has honored Manorhaven Elementary School with the organization's national award for its outstanding efforts in creating an active school environment.
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The Port Washington Education Foundation (PWEF) recently awarded the district 37 grants during the foundation's grant awards ceremony.
With a new school year in motion, the district held its annual new teacher orientation on Aug. 26 and 27 in the Paul D. Schreiber High School library.


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Generations of Energy: August 26, 2013
By Jayne Ann Bugda | firstname.lastname@example.com
Senator Bob Casey is helping local people get jobs in the Marcellus shale industry. Not everyone thinks this is a good idea. Senator Bob Casey says if workers in Pennsylvania don't get gas industry jobs, the jobs will go to other states. That's why he's pushing for laws to help provide gas industry training. Senator Casey was in Montoursville, Lycoming County recently to talk about his proposal. It's called the "Marcellus Shale on the Job Training Act," and he says it'll get specific training to the employees that need it. And it'll help obtain grants to pay for that training. Plus -- the president of the Lycoming County Chamber of Commerce says if the bill passes -- it will help the Lycoming County economy. He supports natural gas efforts ... But not everyone does. Some people in Lackawanna County are speaking out. They say the water in their Dimock community has been poisoned by the gas industry ... .....and they say they have gallons of contaminated water to prove it. Those protestors say they will deliver 50 thousand petitions to officials in Washington DC. Ultimately, they hope the EPA will reopen their investigation on drinking water safety in the area
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Page 1
Alternative Spliced CD1D Transcripts in Human Bronchial
Epithelial Cells
Kambez Hajipouran Benam, Wai Ling Kok, Andrew J. McMichael, Ling-Pei Ho*
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom
CD1d is a MHC I like molecule which presents glycolipid to natural killer T (NKT) cells, a group of cells with diverse but critical
immune regulatory functions in the immune system. These cells are required for optimal defence against bacterial, viral,
protozoan, and fungal infections, and control of immune-pathology and autoimmune diseases. CD1d is expressed on
antigen presenting cells but also found on some non-haematopoietic cells. However, it has not been observed on bronchial
epithelium, a site of active host defence in the lungs. Here, we identify for the first time, CD1D mRNA variants and CD1d
protein expression on human bronchial epithelial cells, describe six alternatively spliced transcripts of this gene in these
cells; and show that these variants are specific to epithelial cells. These findings provide the basis for investigations into a
role for CD1d in lung mucosal immunity.
Citation: Benam KH, Kok WL, McMichael AJ, Ho L-P (2011) Alternative Spliced CD1D Transcripts in Human Bronchial Epithelial Cells. PLoS ONE 6(8): e22726.
Editor: Johan K. Sandberg, Karolinska Institutet, Sweden
Received April 14, 2011; Accepted July 3, 2011; Published August 11, 2011
Copyright: ? 2011 Benam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The study was funded by the Medical Research Council (UK). The funders had no role in study design, data collection and analysis, decision to publish,
or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
* E-mail: email@example.com
CD1d is a member of the CD1 family of transmembrane
glycoproteins. It presents self and foreign glycolipid antigens to a
group ofT lymphocytepopulationcalled naturalkillerT cells(NKT
cells) [1]. In this context, CD1d shows high structural homology to
the MHC class I genes which encodes a type I integral membrane
protein (a heavy chain) with three extracellular domains: a1, a 2,
and a3 [1]. Like HLA A–C, CD1d protein non-covalently
associates with b2-microglobulin (b2m); but unlike the MHC gene,
its expression on professional antigen presenting cells (APC) is
limited in genetic and allelic variation, and is thought to pre-
dominantly engage the semi-invariant T cell receptor (TCR)
expressed by NKT cells. However, the consequence of this
interaction is distinct and wide-ranging. When activated, NKT
cells produce large amounts of cytokines (including TH1, TH2 and
TH17- related cytokines) [2,3,4,5], and much more rapidly than do
conventional T cells. They have the capacity to critically modulate
immunity by interacting with T cells, NK cells, macrophages and B
cells. They regulate the development of a number of inflammatory
diseases – best shown in animal models of Type I diabetes mellitus,
multiple sclerosis, asthma and infectious diseases [6,7,8,9,10,11,12].
In the lungs, divergent effects have been noted after NKT cell
activation. We have shown that NKT cells participate in immune
responses in the lungs, and are protective during influenza virus
infection [11,12]. Their numbers increased in the lungs within
three days of influenza virus infection, and activated NKT cells
can amplify the innate immune response and decrease early viral
load. De Santo et al also showed that NKT cells can reduce the
recruitment of 'myeloid-derived suppressor cells' which allowed
increased proliferation of influenza virus-specific CD8 T-cell
responses [11]. CD1d-deficient mice that lack NKT cells also
succumb to other bacterial infection of the lungs like pseudomonas
aeruginosa [10]. However, NKT cells could also be pathogenic in
non-infectious conditions – for example in the development of
airway hyper-responsiveness in animal models of asthma [9].
Despite these studies, it is not clear how NKT cells arrive in the
andhowtheyareactivated inthe lungs.Intra-nasaladministrationof
a glycolipidligand showed a quick expansion of the cells inthe lungs,
suggesting that a CD1d-expressing cell population could present
antigen to NKT cells in this site [12]. One possibility is dendritic
within the airways. Another possibility is the airway epithelium.
Potentially, CD1d expression on bronchial epithelium could be
involved in activation of NKT cells or promote engagement of NKT
cells with bronchial epithelium and enhance the role of bronchial
epithelium in mucosal immunity. To date, CD1d expression has not
been observed in primary or bronchial epithelial cell lines but it has
been reported on epithelial and parenchymal cells in liver, kidney,
intestine and skin [13,14,15,16,17]. The function of CD1d on these
structural cells is not clear, although freshly isolated intestinal
epithelial cells pulsed overnight with a glycolipid were capable of
activating NKT cells [18]. Here, we provide the first report on
CD1D mRNA and CD1d protein expression in human bronchial
epithelial cells, and describe six alternatively-spliced transcripts of
this gene in these cells. This provides a basis for investigations into a
role for CD1d in lung mucosal immunity.
Primary human bronchial epithelial cells and airway
epithelial cell lines express CD1D
Three sets of primers (''A/A'', ''B/B'' and ''C/C'') were
designed to specifically amplify CD1D transcript within its coding
PLoS ONE | www.plosone.org1August 2011 | Volume 6 | Issue 8 | e22726
Page 2
exons (Table 1, and Figure 1A). ''A/A'' spanned a2 exon covering
most of a1 and a3 exons, and ''B/B'' amplified transmembrane
(TM) and most of a3 and cytoplasmic tail (T) exons. We first
determined if human bronchial epithelial cells expressed CD1D,
using RNA derived from ex vivo primary human bronchial
epithelial cells obtained by bronchoscopic brushing. These brushed
cells comprised 90% epithelial cells, as shown by pancytokeratin
and ß tubulin IV immunofluorescense staining (Figure 1B), and
werefound to express CD1D (Figure 1C,right panel). SinceCD1D-
expressing cells may have contaminated this population of primary
epithelial cells, we proceeded to examine CD1D expression in pure
airway epithelial cells using the Beas2B cell line. RT-PCR detected
CD1D transcripts in Bea2B (Figure 1D). The Jurkat cell line, which
naturally expresses CD1d protein, was used as a positive control.
Absence of contaminating DNA was shown by including a reaction
where no reverse-transcriptase was used ('No RT').
Expression of CD1d protein on epithelial cells was
observed with some but not all CD1d mAbs
Using three mouse anti- human CD1d mAbs (clones CD1d 43,
CD1d 51.1.3 and NOR 3.2), we showed surface CD1d expression
on Beas2B, primary brushed bronchial and NHBE cells with all
three mAbs; but, A549 – a Type II human pneumocyte, stained
positive with one Ab only (Figure 2A–C). The clone 51.1.3 had
been previously reported to partially cross-react with CD1b [19].
To verify CD1d detection, Beas2B cells were stained with an anti-
CD1b mAb. We found no CD1b expression on Beas2B, whereas
the positive control, monocyte-derived dendritic cells (MD-DCs)
distinctly expressed CD1b (Figure 2B).
The epitopes for these CD1d mAbs are currently unknown.
However, the differential expression raised possibility of the
existence of variants and prompted design of further primer sets to
examine this prospect.
RT-PCR of primary bronchial epithelial and cell line
confirms expression of CD1D in bronchial epithelium and
reveals six variant transcripts
To explore the possibility of splice variants, primers were
designed to probe the exon boundaries (Figure 3A) (primer pairs
''C/C'', ''D/D'' and ''D/E''). Using primer pair ''C/C'', which
amplifies a 572–bp product containing parts of the signal peptide
(S) and a2 exons, and the whole a1 exon of CD1D, we unex-
pectedly, observed a 305-bp product in brushed primary bronchial
epithelial cells (Figure 3B, left panel), which raised the possibility of
an alternatively spliced variant. This was also the case in the
Beas2B cell line (Figure 3B, right panel), where both the full-length
and 305-bp products were amplified. Direct nucleotide sequencing
confirmed the shorter amplicon to be CD1D with a spliced a1
exon, which we called ''V1'' (Figure 3B, bottom panel). To ensure
that V1 is not a non-specific amplification, RT-PCR using the C/
C primer was repeated on cDNA reverse transcribed using a
CD1D-specific primer (GGACGCCCTGATAGGAAGTT) rath-
er than oligo dT. The latter also showed the presence of V1
(Figure 3B, right panel).
For ''D/D'' and ''D/E'' primers, the forward primer in both sets
originates in the first half of the a2 exon, while the reverse primers
Rev. D and Rev. E originate in T (tyrosine tail) and 39-UTR of exon
7, respectively (Figure 3A). RT-PCR with ''D/D'' amplified six
products with varying lengths (Figure 3C). Only one (Non-Spliced;
NS) corresponded to expected non-spliced transcript (636-bp). To
examine whether the other amplified DNAs were spliced forms of
CD1D, the bands were cut out from the gel and nucleotide
sequence determined. This confirmed all as PCR products for
CD1D (''V2–6''). In addition, sequencing revealed the splice
junctions for three of the variants: ''V4'', ''V5'', and ''V6''
(Figure 3D). V4 and V5 were deficient in a3 (279 bases) and a3-
TM (379 bases), respectively; whereas ''V6'' represented a variant
with double splicing events, trimming out the second half of a2 (last
133outof 279 bases)and most (274 out of279bases)of the a3 exon.
To compare the relative amount of variants, qPCR using SYBR
Green methods or Taqman probe was carried out for ''V1'', ''V3–
6'', using the primer and probe sets described in Table 1. Multiple
primers for ''V2'' produced late Ct on amplification plot and were
inconsistent. We believe this reflected very low level of gene
expression and therefore did not test V2's relative levels. We tested
these primers in NHBE primary cells (Figure 4A, left panel). This
allowed us to confirm the variants in primary human bronchial
epithelial cells and also show that relative to the alpha-1 deficient
splice variant (''V1'') (Figure 4A), ''V4'' and ''V6'' were most
highly expressed. Relative to the full length transcript (FL), ''V1''
was 9.2 fold lower (Figure 4A, right panel). Characteristics and
potential implications of ''V1–6'' are summarized in Table 2.
To exclude possibility of DNA defects as the source for these
variants, genomic DNA from Beas2B were amplified using two of
the primers and showed to have only one band (Figure 4B).
Table 1. Primers and probes used in this study.
Target genePrimer or Probe sequence (59R39)
CD1D For. A: CTCGTCCTTCGCCAATAGC
Rev. A: ACCACATCCAGGGTTGCTC
For. B: AGCCTGTATGGGTGAAGTGG
Rev. B: GGACGCCCTGATAGGAAGTT
For. C: CTGCTGTTTCTGCTGCTCTG
Rev. C: GACTCAAGGAGGCCACTGAC
For. D: CTGGGAACGCCTCAAATAAC
Rev. D: ACAGGACGCCCTGATAGGA
Rev. E: GAAAGCTGCCTCATGACTGTT
GAPDHFor. GAGTCAACGGATTTGGTCGT
Rev. TTGATTTTGGAGGGATCTCG
CD1DFor. V1 (SYBR): GCGCTGAAGATCCCTTGGA
Rev. V1 (SYBR): TACATGGAAGAAGTTATTTGAGGCG
For. V3 (SYBR): CAAGAGGCCCCACTTTGGT
Rev. V3 (SYBR): GAGACATGGCACACCAGCAG
For. V4 (Taqman): AACAGTGCAGTGGCTCCTTAATG
Rev. V4 (Taqman): TCCCACCTTGCTTCTTCAGTTC
Probe V4 (Taqman): CCCAATTTGTCAGTGGCCTCCTTGAGT
For. V5 (SYBR): GAGGCCCCACTTTGGGTAAA
Rev. V5 (SYBR): CAGGACGCCCTGATAGGAACT
For. V6 (Taqman): TTTGGAGCAGGTGGGAGCTA
Rev. V6 (Taqman): CGGGAGGTAAAGCCCACAAT
Probe V6 (Taqman): AGTCCTGGCGTGCTTGCTGTTCCTC
HPRTFor. (Taqman): GACTTTGCTTTCCTTGGTCAGG
Rev. (Taqman): AGTCTGGCTTATATCCAACACTTCG
Probe (Taman): TTTCACCAGCAAGCTTGCGACCTTGAT
For.: Forward primer; Rev.: Reverse primer; SYBR: real time PCR amplification by
SYBR Green method; Taqman: real time PCR amplification by Taqman assay
using FAM-, TAMRA-conjugated primers; V1–6: CD1D spliced variants.
CD1d Expression on Bronchial Epithelium
PLoS ONE | www.plosone.org2August 2011 | Volume 6 | Issue 8 | e22726
Page 3
CD1D spliced variants are expressed on epithelial cell
lines but not in antigen presenting cells, and fibroblast
Although we have shown existence of CD1D variants in human
bronchial epithelial cells, it is unclear if the same set of variants is
observed in other cells. Unlike other CD1 family members (CD1A,
CD1C, and CD1E), CD1D splicing has only been marginally
studied in two reports [20,21]. One study found eight variants in
peripheral blood mononuclear cells (PBMCs), but using nested
PCR which reduced the reliability of the results [20]. Neither
study compared the expression of the spliced variants across
different cells. To address this, we examined CD1D variants in
several cell lines representing epithelium from various sites
(Beas2B, 16HBE14O2, NKer, HaCat, HCT116 and TZM-bl;
sources described in Methods) compared to antigen presenting
cells (THP-1), T cells (MOLT4) and fibroblasts (MRC5 and
IMR90). Using a primer set that identified variants ''V2–6'' (''D/
D''), we found similar splicing patterns among bronchial epithelial
cells 16HBE14O2and Beas2B, normal keratinocytes (NKer) and
colon epithelial cells (HCT116) (all expressed full length and ''V2''
to ''V6'' variants). This suggests potential similarity in splicing
machinery at resting state across different mucosa-lining epithelial
cells, though cancerous transformation or immortalised nature of
cell lines may account this phenomenon The fibroblasts only
expressed ''V6'' (MRC5) and ''V3'', ''V4'' and ''V6'' (IMR90).
The primary cells PBMC and dendritic cells (DCs) also expressed
all variants but possibly to a different relative amounts (Figure 4D).
The a3–deficient CD1D variant (''V4''), when present, was the
most intensely PCR-amplified transcript in all cell types.
In this paper, we show that CD1d is expressed on bronchial
epithelial cells, both in primary and airway epithelial cell lines, and
that there are at least six spliced variants likely specific to epithelial
cells. We felt that examining the function of these epithelial CD1d
transcripts was beyond the scope of this paper so it is unclear
currently how these proteins function in the context of the lungs.
However, it is possible to speculate on the potential implications of
CD1d expression on these structural cells. Due to the large surface
area of the airway epithelium, one prospect of CD1d expression
here is the rapid presentation of both self and exogenous
glycolipids to infiltrating NKT cells. This could maintain NKT
cells proliferation in situ and prolong their protective effect in the
lungs. The opposite is also possible – that of enhancing NKT cell's
harmful effect e.g. airway hyper-responsiveness observed in
murine models of asthma. In the ovalbumin asthma model,
Figure 1. Identification of CD1D transcript in human respiratory epithelial cells. Panel A: Graphical representation of CD1D mRNA; coding
exons in orange and non-coding exons in open boxes. Primer annealing sites are illustrated by horizontal arrows, with the expected amplicon size for
each pair. Panel B: Brushed bronchial cells immunostained with pancytokeratin and b–tubulin IV, identifying epithelial cells and cilia respectively;
''isotype control'' - no primary Ab treatment. The 2006magnification shows ß tubulin IV staining in green and nuclei in blue (DAPI). Panel C: Agarose
gel on the right demonstrates RT-PCR on these cells. Panel D: CD1D expression on Beas2B and Jurkat cell lines.
CD1d Expression on Bronchial Epithelium
PLoS ONE | www.plosone.org3 August 2011 | Volume 6 | Issue 8 | e22726
Page 4
CD1d2/2mice do not develop airway hyper-responsiveness
A potentially interesting part of our results is the observation of
the same profile of transcripts in epithelial but not haematopoietic
cells. The epithelial cell lines from skin, lungs, cervix and colon
showed ''V2'' to ''V6'' expression (''V2'' and ''V3'' very faint in
the cervical epithelial cell line, TZM-bl). The exception to this is
A549, a type II alveolar epithelial cell line which only expressed
''V4'' and ''V5''. Type II alveolar epithelial cells have the unique
property of producing surfactants, which regulate the surface
tension of the alveoli and also to contribute to host defence. In the
latter, two subsets of surfactant (SP-A and SP-D, part of collectins
family) act as opsonins to facilitate elimination of pathogens by
alveolar macrophages [25]. The CD1D transcripts on these cells
(''V4'' and ''V5'', discussed later as potential ß2m-independent
and soluble forms respectively) could serve as innate host defence
molecules rather than antigen-presenting molecules.
Against expectation, we observed only very low/undetectable
levels on full length mRNA in PBMC and DCs (Figure 4C). There
have been very few studies on the variety of CD1D mRNAs in
these cells and their relative expression to the full-length transcript.
Kojo et al mentioned existence of multiple CD1D transcripts in
PBMCs, however, the level of expression was so low that they
required nested PCR to identify this; only three healthy volunteers
were studied, among these, one had extremely low/undetectable
levels of non-spliced full-length CD1D. Our low expression is in
keeping with their finding, and the low/undetectable expression
by one-round of PCR in MOLT-4 and THP cells support the
finding in primary hematopoietic cells Since the CD1d protein has
been identified in these cell populations, one possibility is that the
mAbs used detected proteins translated by the variants rather than
the full length CD1D.
Some potential functions of the different variants could be
gleaned from knowledge of the various regions that are spliced
(Table 2). Lipid-binding groove in CD1d is formed by a1 and a2
domains [26,27]. The protein for ''V1'', deficient in a1, is unlikely
to participate in antigen loading and presentation. Hydrophobic
amino acid residues encoded by transmembrane (TM) exon are
Figure 2. Human respiratory epithelial cells express CD1d protein. Panel A: Flow cytometry analysis of CD1d expression on Beas2B and A549
epithelial cell lines, using three anti-human CD1d mAbs – clones 42, 51.1.3, and NOR3.2. Panel B: CD1b staining on Beas2B cells and CD1b-expressing
monocyte-derived dendritic cells (DC). Panel C: Flow cytometry analysis of CD1d expression on primary human lung epithelial cells (top panel) and
normal human bronchial epithelial (NHBE; bottom panel) cells.
CD1d Expression on Bronchial Epithelium
PLoS ONE | www.plosone.org4August 2011 | Volume 6 | Issue 8 | e22726
Page 5
required for insertion of the CD1d protein into plasma membrane.
The TM exon is spliced out in ''V2'' and ''V5''. In addition, ''V3''
and ''V6'' as a consequence of shift in reading frames contain early
stop codons in a3 and TM, respectively. So, it is likely that
proteins coded by these variants will be either secreted (soluble
isoform) or retained intracellularly. There is precedence for this -
Woolfson et al showed expression of CD1A and CD1C TM-
deficient transcripts, and detected corresponding soluble protein
isoforms [28]. Similarly, soluble HLA-G, an MHC class I
molecule which has limited polymorphism and shares high
structural similarity with CD1D [29], is encoded by a TM-
deficient variant [30,31], and was recently shown to activate NK
cells via NFkB signalling [32].
''V4'' is the most highly expressed transcript in NHBE cells; and
is potentially an interesting variant which could have epithelial-
specific function. Its protein product is predicted to lack ß2m
binding ability but it has intact TM and cytoplasmic tail (T) exons,
so it can produce a cell surface-expressed b2m-free CD1d isoform.
On human intestinal epithelial cells, the major form of CD1d is a
non-glycosylated, ß2m-independent molecule, although these cells
also express a ß2m-associated, fully glycosylated form of CD1d
[30]. The importance of ß2m association with CD1d with respect
to the CD1d biosynthetic pathway is not established. Association
of ß2m with CD1d could have a role in regulating the extent of
CD1d glycosylation and the maturity of the attached carbohydrate
side chains [33]. Although, in most cases, forms of MHCI protein
that do not associate with ß2m do not fold their antigen presenting
domains properly, cells from ß2m -deficient mice are able to
stimulate proliferation of CD1d-restricted T cell clones [34],
suggesting presence of a CD1d variant that do not rely on ß2m for
antigen presentation and function.
Regardless of the transcripts, identification by antibodies
confirms the presence of protein on the surface of bronchial
epithelial cells. The CD1d antibody, clone 42, has been widely
used as a blocking antibody to prevent activation of NKT by
CD1d-expressing cells [35,36,37], therefore flow cytometry results
Figure 3. Expression of six CD1D alternatively spliced CD1D variants in human respiratory epithelial cells. Panel A: Schematic
illustration of primer pairs ''C/C'', ''D/D'', and ''D/E'' annealing sites on CD1D mRNA, and corresponding full-length product sizes. Panel B: RT-PCR on
primary bronchial epithelial cells detected a 305-bp band. Same primers amplified the expected full-length (572-bp) product plus a shorter amplicon
(305-bp) both in oligo dT– and CD1D specific primer–reverse transcribed cDNA from Beas2B. Direct nucleotide sequencing of the smaller band (lower
panel) identified a CD1D variant lacking a1 exon (''V1''). Panel C: Primer pairs ''D/D'' and ''D/E'' detected five other CD1D variants (''V2–6'') in Beas2B.
Panel D: Direct nucleotide sequencing of ''V4–6'' revealed the splicing junction in these variants.
CD1d Expression on Bronchial Epithelium
PLoS ONE | www.plosone.org5August 2011 | Volume 6 | Issue 8 | e22726


text:
January 14, 2014
The California Air Resources Board is reminding brokers, forwarders, shippers and receivers that hire motor carriers to transport perishable goods on California highways to re-establish due diligence in their hiring practices with the start of 2014.
That's because Dec. 31 was the deadline for model year 2006 transport refrigeration unit and TRU generator set engines to meet state in-use performance standards. As a result, there may be some noncompliant TRU and TRU generator set engines that cannot legally operate in California.
"Any business entity that hires motor carriers to transport perishable goods on California highways and railways must require the carriers they hire or contract with to only dispatch TRUs or TRU generator sets that comply with the TRU's in-use performance standards," said CARB in an advistory.
CARB's TRU Advisory 13-25 explains what this means for the hiring business entities, such as freight brokers, forwarders, shippers and receivers that hire refrigerated motor carriers. It lists some strategies that a hiring business entity may use to make sure they only hire motor carriers with compliant TRUs.
Also in 2014 CARB plans to check refrigerated carrier drivers for the documentation they are now required to carry. The TRU regulation requires:
The TRU Advisory is available online.
Guidance for brokers, forwarders, shippers, receivers, carriers and drivers for hiring and contracting TRU-carrier transport of perishable goods on California highways and railways is also available online.
For general information about the TRU Regulation, you can visit the TRU website.
If you have questions about compliance or registration you can call the TRU help line at 1-888-878-2826 or 916-327-8737.
We offer e-newsletters that deliver targeted news and information for the entire fleet industry.
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McLeod Offers 'Branded' Driver App for Fleets
Paccar Announces MX-11 Diesel for North America


text:
Jeremy Clarkson, Richard Hammond and James May will soon debut their brand new automotive program on Amazon Prime, and it looks like this "new era of Clarkson, Hammond and May" will kick off with something big.
That "something big" is what we waited for since the last season of BBC TopGear - a side-by-side comparison test of all three hybrid hypercars - Ferrari LaFerrari, McLaren P1 and Porsche 918 Spyder.
As far ...more
Yesterday evening I went from bored to excited to disappointed and back to bored again in space of one hour. The source of my excitement was unexpected release of second DLC for my favorite driving simulator - Assetto Corsa.
The "Dream Pack 2" DLC includes a new track - Circuit de Catalunya Barcelona and, more importantly, 8 new cars - Lamborghini Countach 5000 QV, Lamborghini Huracan GT3, Ford GT40 MK1, Scuderia ...more
The "M3 GTS" nameplate turns out to be more than just a single generation oddball. The Bavarian automaker continues the "GTS" lineage with BMW M4 GTS - more track focused, limited edition F82 coupe.
The biggest news is that BMW claims M4 GTS has achieved a sub 7:30 laptime at Nurburgring Nordschleife.
7:28 laptime puts the relatively "mundane" 3-series-derived coupe in exotic company, and places it slightly above "track greats" like Porsche ...more
added 0 - 200 kph for Ferrari F12 TDF and top speed for Ferrari F12 TDF
added laptime for Ferrari F12 TDF, 0 - 100 kph for Ferrari F12 TDF and Ferrari F12 TDF


text:
Thanks for helping us associate the correct Pornstars to this video!
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All Comments (14)
che gran figa la signora .sborrrooooooooooooooooooooooooooooooooooooooo
Not much in the way of dialogue. She looks nice.
She looks a bit like my slut wife but it can't be because she would be shagging a black cock not a white one.Swallows like mine as well.
she is one fine hot looking women!
she loves warm cum in her mouth
sei troppo bona ti sccoperei io
Name = Marylin Daha
Was fuer ein scheiss der Typ die ganze Zeit schwaetzt aber ansonsten geile fotze
the moaning is hysterical. sounds like 70's porn without the corny music...
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After the fellatio, there was a 69 position which could have been followed by a scene where SHE put HIS erect penis into her warm, moist waiting vagina. That did not happen at 10 minutes and 19 seconds. The next scene was a doggy style pose and a cow girl position could or should have had a scene where she put his penis into her vagina. Was this just another film showing how a man gets off with the use of a woman as an accessory?
If I was her age, and had a body like hers, and still enjoyed cock - like she does - I'd want to fuck every young stud I could get. If she can still do it with gusto, we will all watch her fucking herself silly. I think she's had enough after 20 minutes, though...


text: rachel loves niall, Twitter [ What Is the 'Charlie Charlie Challenge' Why Has It Taken Over the Internet? By TheFW Staff June 4, 2015 | TheFW While you were out enjoying a three-day Memorial Day weekend, the internet was enjoying a new craze. Read More|Comment Category: National News, Weird News Tags: Internet, paranormal, religion, social media, superstition Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


text:
iPad apps in the classroomMore Related Discussions
This post was created by a member of Edutopia's community. If you have your own #eduawesome tips, strategies, and ideas for improving education, share them with us.
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I agree. Frog Dissection is a great app. Also, there is an app for Rat Dissection as well. I am interested in other ways science teachers are using iPads in their classroom.
I use Cozi, which has a wonderful app for personal organization. There is also inClass which has a calendar, and will save notes in a pdf format. I use a couple of different local news and weather apps, the Kindle, USA Today, Pages and Numbers, a sudoku app, and Sticky Notes. A friend also recommended QuickOffice. I have students who are using most of these in some form or another. Our school district also subscribes to Google Docs for employees and students, so we are able to access these from netbooks, touchpads and iPads as well as traditional laptops and desktop computers. I also have students who really like iTunes U.
I have tons of organized links to apps and "all things" iPad here--
I use the Splashtop app to remotely control my desktop when I need greater functionality than a remote clicker offers. This way I can interact with the computer while I'm with the kids.
I also use a function generator app (Tone Generator Pro) to output pure tones to the speaker on my Ruben's tube and the standing wave generator.
I, too, have installed Splashtop and am looking forward to using it in my library. It is so much better than the interactive tablets because I can actually see the computer screen on my iPad.
I'll also be showing my students the various citation apps such as EasyBib, iCitation, and QuickCite.
My kids like Fruit Eater and The Jungle Book
Since iPads and most other tablets have web browsing features, I think we should also consider the world of web-based apps for education.
My teacher colleagues are very fond of Educreations and Smarter Cookie, both tools that help create engaging lessons. Also, 123ContactForm's web form builder can help creating great quizzes and teacher forms, both in class and outside of it.
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text:
Complete Farscape on DVD Nov 17
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November 19 2009, 04:13 PM
Fleet Captain
Location: Wisconsin
Re: Complete Farscape on DVD Nov 17
So what is the situation with the Farscape picture? Did A&E screw it up? Because when the Premiere came out I don't remember it being in widescreen.
LitmusDragon wrote:
I love the packaging on this set. The individual season ... boxes? (probably some technical term here I am forgetting) are quite thick and sturdy and the disc releases inside the boxes are the high-quality kind. I wish the DS9 and Voyager sets had this same level of quality. Voyager in particular had some really crappy packaging.
You are right about that at least R2 got good packaging for the slimline editions.
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Walmart Electronics Associate Interview Questions
Interview Experience
Interview Experience
Getting an Interview
Getting an Interview
Interview Difficulty
Interviews for Top Jobs at Walmart
- Sales Associate (389)
- Wal Mart Cashier (326)
- Cashier (208)
- Overnight Stocker (197)
- Assistant Manager (155)
- Cashier Associate (108)
- Assistant Store Manager (93)
- Walmart Cashier (67)
- Department Manager (64)
- Pharmacy Technician (49)
- Guest Service Team Member - Cashier (42)
- Associate (40)
- Customer Service Manager (40)
- Electronics Sales Associate (37)
- Stocker (35)
- Shift Manager (31)
- Asset Protection Associate (31)
- Manager (30)
- Deli Sales Associate (25)
- Customer Service Manager (CSM) (25)
- Customer Service Associate (24)
- Customer Service Representative (21)
- Deli Associate (20)
- Area Manager (20)
- Inventory Associate (20)
- Store Manager (19)
- Electronic Sales Associate (19)
- Courtesy Associate (18)
- Certified Pharmacy Technician (18)
- Any (17)
10 Candidate Interview Reviews Back to all InterviewsSort: Popular Date Difficulty
Electronics Associate Interview
I applied online. I interviewed at Walmart.
Was easy mostly ethical questions, the supervisor who interviewed me was very friendly. They as question about how would you react when something happen and what if an angry customer came to you.
Interview Questions
- Tell me about a time you had a problem and solved it. Answer Question
Other Interview Reviews for Walmart
Electronics Associate InterviewAccepted OfferPositive ExperienceEasy Interview
I applied through an employee referral. The process took 1 day. I interviewed at Walmart.
had one interview and it was simple and straightforward. They interview you in a group, meaning you and like 3 managers. They ask basic questions like "if you were in a bad situation what would you do" or "name a time that you resolved a conflict"
Interview Questions
- no difficult questions Answer Question
Electronics Associate InterviewAccepted OfferPositive ExperienceEasy Interview
I applied online. The process took 2 weeks. I interviewed at Walmart (Shakopee, MN).
BEFORE the questions be sure to briefly mention about any skills that you have that are relevant to the job. Answer basic questions about your past experiences. If you have any gaps, use any reasonable answer, the only wrong answer is not having one. And I can't stress this enough, if you are not honest about when you are available and change it immediately, the system may not give you very many hours, and your supervisors will not like you.
Interview Questions
- People seem to always not include experience on their resume's, include EVERYTHING. 1 Answer
No negotiation on hourly positions, I don't know about salary, but you CAN talk about promotions and different positions available.
Electronics Associate InterviewAccepted Offer
I applied online and was called within a few weeks for an interview. I originally interviewed with someone who was forced out of her position by a power-hungry assistant manager. Following that, I interviewed with an assistant manager. Both interviews were fairly easy and generic. It's been a while since all of this, so I don't remember much else.
Interview Questions
- What was your most difficult encounter with a customer? Answer Question
Electronics Associate InterviewNo OfferNegative ExperienceAverage Interview
I applied online. The process took a week. I interviewed at Walmart in April 2013.
I got called for an interview. I went in, and proceeded to go through two interviews in the same day, neither of which I feel had questions that were relevant or very enlightening to the interviewer. The first person who interviewed me was not part of the department I was interviewing for, nor had they had a good deal of experience with interviewing. The reason I know this is because they told me it was their second time ever interviewing someone. I found the process along with the material of the interview to be ridiculous. Each question had several questions contained within it, which was very confusing and hard to keep track of. Also, the questions were so vague that it was hard to develop cohesive answers to them. I was not even done after two interviews. I was scheduled to do a third interview, but I declined as I thought it was ridiculous to go through three interviews at a place where half the people I saw working looked like they didn't want to be there. Walmart, you need to rethink your process.
Interview Questions
- All of them were difficult. I cannot remember any specifics though, as they were mostly vague, run-of-the-mill interview questions. Answer Question
Electronics Associate InterviewAccepted OfferPositive ExperienceDifficult Interview
I applied in-person. The process took 4 days. I interviewed at Walmart (Summerville, SC) in August 2012.
Pretty simple really.You fill in the application.You do so by going to a Kiosk usually set in the customer service area.You will receive a call to come in by a certain time for interview.(Important :Be on Time).They usually give you an interview.They may not call you back right away (for me it was a day or two between when I made the application and when they called me).If you get a call back to have a 2nd interview it's pretty safe to say you have the job.The second one is usually filling out the paperwork, doing your drug test, and getting the anti union stuff out the way.
Interview Questions
- They seemed to be fixated on whether or not you will go the extra mile for the company.So they may ask you to name a time when you did a extra project for your previous employer.Or can you tell me a time you've done more than what was expected of you on a project and what was the result? 1 Answer
Electronics Associate InterviewAccepted OfferPositive ExperienceEasy Interview
I applied online. The process took a week. I interviewed at Walmart (Cypress, TX) in April 2012.
I applied and about a week later i received a call back from the Personnel Office. Depending on if the store is hiring or not will determine how quickly they respond.
Interview Questions
- There were really no difficult questions. They just ask simple questions like: Describe a time you had to handle a difficult task, or if a customer is angry how would you handle the situation? Answer Question
Electronics Associate InterviewAccepted OfferNeutral ExperienceAverage Interview
I applied online. The process took 6 days. I interviewed at Walmart (York, PA) in February 2009.
I applied online and was called within a week and asked to interview the same day. I was offered the position, they called my references immediately, and asked me to come back to do paperwork and get form for drug test. I met the department manager and an assistant manager. I worked as a communications consultant and freelance designer for 20+ years, but had never worked retail. I had to provide answers to questions based upon my experience in other arenas, and found that my interviewers did not relate to the situations I described. I did not get "job credit" consideration on starting wage as 20+ years of professional self-employment was not considered applicable to this job.
Interview Questions
- How did you... Deal with a difficult customer. Act in a leadership role as part of a team. Provide good customer service. Solve an unexpected problem. Answer Question
There is no negotiation with regards to wage or position offered, even if there is more than one job open at the time of your interview.
Electronics Associate InterviewAccepted OfferNeutral ExperienceEasy Interview
I applied online. The process took a week. I interviewed at Walmart in July 2010.
its a joke they are so desperate for people they will hire about anybody that can pass drug test and background check. Expect to answer set questions that doesnt matter how you answer and also expect them to pay you the least amount possible. Also watch out for the 40 hour a week part time job offer.
Interview Questions
- Name a time when you had to work fast without sacrificing quality. Answer Question
would not negotiate
Electronics Associate InterviewAccepted OfferPositive ExperienceDifficult Interview
I applied online. The process took 1 day. I interviewed at Walmart in May 2010.
When I applied to Walmart, I completed a test online. I received a phone call from an assistant manager on a Friday morning, and was scheduled for a Monday interview. I was interviewed by 3 people; assistant manager, zone manager and merchandise supervisor. I was asked scenario questions, mostly "tell me about a time when..." The 3 of them met afterward, briefly, and the assistant manager asked me to come back later in the week for a drug test. I did that Thursday, and was hired on Friday.
Interview Questions
There was not any negotiation. I was presented with an offer and I chose to accept it.
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text:
MONDAY, July 28, 2014 (HealthDay News) -- Babies born prematurely appear to have a slightly increased risk of potentially fatal blood clots that they will carry into adulthood, Swedish researchers report.
Doctors have previously suspected that babies born earlier than 37 weeks' gestation have a raised risk of deep vein thrombosis and pulmonary embolism, two serious conditions caused by blood clotting in the veins, the researchers noted in background information.
This new study confirms that link, and takes it even further. Premature birth appears to be linked to an increased chance of blood clots in the veins in childhood and early adulthood, according to findings published online July 28 in the journal Pediatrics.
The researchers also reported that a baby's chances of blood clot-related illnesses are directly related to the degree of prematurity. "The more premature, the higher the risk," said Dr. Edward McCabe, chief medical officer of the March of Dimes. A full-term pregnancy lasts from 39 to 40 weeks.
While parents and doctors should keep this risk in mind, they should also be aware that the risk is not huge, said Dr. Kristi Watterberg, chair of the American Academy of Pediatrics' committee on the fetus and newborn. Watterberg and McCabe were not involved with the study.
The association between premature birth and clot risk seen in the study does not prove a cause-and-effect relationship.
The study involved 3.5 million babies born in Sweden between 1973 and 2008, including almost 207,000 born preterm. Out of all the births, only about 7,500 children -- 0.2 percent -- suffered either deep vein thrombosis or pulmonary embolism later in life.
"I think it's important scientifically to know, but it's such a low inc
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text:
Star by Julien Macdonald Set of 2 hand crafted 'Heart' champagne flutes
Item No.3340239013
Item No.3340239013
This set from Star by Julien Macdonald comes with two luxury designer champagne flutes, both of which are hand crafted from glass with with carved heart patterns and Swarovski Elements crystal detailing.
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In This Section
Journal Forum
Synthetic Reverberator - January 1960
1 comment
Multichannel Sound Reproduction Quality Improves with Angular Separation of Direct and Reflected Sounds - June 2015
1 comment
Clean Audio for TV broadcast: An Object-Based Approach for Hearing-Impaired Viewers - April 2015
2 comments
AES E-Library
An Integrated Solution For Digitization, Archiving, And Restoration Of Large Audio Collections
There is a growing demand to rescue large historic audio collections stored on different analogue media. The huge amount of historic recordings in audio libraries would take hundreds of years for high-quality transfer to new reliable digital media. Non-repairable defects can occur in the meantime, so this is obviously too long to wait. It is also too expensive, because very qualified experts are required to achieve professional results. This paper describes a system "already implemented as a prototype" that allows transferring and archiving large audio collections faster, easier and more cost effective. This system is also suitable for automatic embedding metadata coming from existing database systems, handling large wave files (over 2GB), and automatic restoration of digitized and archived audio files.
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