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#651 of 1387
Lewiston, Maine,
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Frozen Balls Four
Bob Orsillo
Photograph - Original Fine Art Photography By Bob Orsillo
Frozen Balls Four - Original fine art abstract macro photography by Bob Orsillo
Golf balls frozen in ice
Copyright (c)Bob Orsillo / http://orsillo.com - All Rights Reserved.
Buy art online. Buy photography online
September 27th, 2013
Viewed 827 Times - Last Visitor from Seattle, WA on 10/05/2015 at 1:43 AM
Sales Sheet
More from Bob Orsillo
Starachowice, sw - Poland
Brilliant work,Bob!Wonderful ,red freezer! LF
Little Falls, MN - United States
Hi Bob, Great photo with RED and interesting texture of frozen bubbles. This image would look great in the group "Loving the Color Red" and get more promotion. L/f
Corvallis, OR - United States
I call this "Golf With A Passion" ... Excellent freeze art Bob! Cheers to you in 2014 ;-)
Fife, Sc - United Kingdom
Bob this a wonderful series and this is a fine example why. v/f
Kailua Kona, HI - United States
Love the energy and beautiful colors in this image! L/F
Walnut Creek, CA - United States
Looks like ice in a glass of coke, very cool
Bradenton, FL - United States
Gorgeous, vibrant color and tones and interesting textures. Nice work, Bob. v26/fv
Weston, FL - United States
Such great colors and energy on this art. Great work Bob!
Kamloops, BC - Canada
What creativity and innovation! This is fascinating to look at, Bob! Very cool! V
Port Hardy, BC - Canada
Love it...very cool to say the least. f/v
Staffordshire - United Kingdom
makesa brilliant abstract :)
Strykersville, NY - United States
From the mundane to the spectacular - only you could do this so effectively Bob. This gives new meaning to macro photography - it's almost like time lapse of a whole new world. The universe in microcosm. The way the light reflects off the bubbles and edges is just amazing. I could get lost in this world. F/V FB T G
Sioux City, IA - United States
Fantastic abstract Bob, beautiful colors love all the bubbles!! f/v/t/g+
Sumner, IA - United States
San Francisco, CA - United States
Fabulous bubbly abstract! f/v
Grants Pass, OR - United States
Very cool image, my friend!! :) F/V
Sayre, PA - United States
So eye catching image! Love the textured and bubles detail! F&V
Long Beach, CA - United States
Beautiful! I love all the texture and colors! f/v/tweet/su/g+
Doniphan, MO - United States
So beautiful and amazing! Wonderful bubble details, love the color as well! So creative! v.f
London, mi - United Kingdom
wonderful vibrant shot Bob !!! wonderful!!! fv
Sussex, WI - United States
The macro world opens so many wondrous possibilities as in this fine work, well done, Bob! v/f
Santa Rosa, CA - United States
Yes, another W O W factor Bob!!! f/v
Wayne, PA - United States
This is fantastic! VF!!!
Bucharest, Ro - Romania
Wow ! Soooooooooooo rich and cool ! Love the red tones in this extraordinary abstract photo, my friend. f,v, SU
Greensboro, NC - United States
Stunning abstract, light dance in the bubbles, another original, Bob, beautifully done capture!! f/v.
Singapore, SG - Singapore
Wow! What a skillful take, Bob! I love the way the bubbles appear in different sizes all over and in different tones of red too!
Milwaukee, WI - United States
Bob-- I love this whole frozen ball series. Fascinating experiments. V/F
Vancouver, BC - Canada
Oh this looks yummy! And I love red!!f/v/tw/g
Daytona Beach, FL - United States
I love this Bob!!! f/v/t
South Lake Tahoe, CA - United States
Cool Bob Looks like something from outer space. V
New York, NY - United States
Very weird idea with really great results!
North Windham, CT - United States
WOW!! This is amazing!! F/V
Milwaukee, WI - United States
This is pretty cool Bob, so original...love it!!!! f/v
Please Wait...
Beverly Hills, CA (United States)
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Singer Robin Thicke is definitely improving with age. See his evolution from bushy hair to "Blurred Lines."
Photo: Getty ImagesMore from Robin Thicke
Photo: Wire ImageMore from Robin Thicke
Photo: Youtube
Photo: Splash NewsMore from Robin Thicke
HAIM: 1989 Tour Pre-Party
Wiz Khalifa Meet + Greet
Tove Lo at The Verb Hotel

subset: commoncrawl


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Town Square
Police arrest suspect in downtown groping
Original post made on Jun 11, 2013
Read the full story here Web Link posted Tuesday, June 11, 2013, 1:08 PM
Comments (3)
a resident of Another Palo Alto neighborhood
on Jun 11, 2013 at 2:25 pm
Got to wonder what this guy was thinking about when he stopped his car?
a resident of another community
on Jun 11, 2013 at 4:29 pm
Hide yo kids, hide yo wife!
a resident of Green Acres
on Jun 11, 2013 at 9:46 pm
Brave determined woman. Way to go! Get the police to tell you the loser's address and get a bunch of friends to go there and kiss his ass.
Glad you are safe!!! Thank you for reporting this incident.
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Mademoiselle Colette, now open in downtown Menlo Park
By Elena Kadvany | 13 comments | 2,850 views
By Chandrama Anderson | 0 comments | 946 views
The Big Four - Part one
By Laura Stec | 2 comments | 750 views
How early is too early to make or buy a Halloween costume
By Cheryl Bac | 9 comments | 497 views
Breakfast for dinner
By Sally Torbey | 9 comments | 487 views


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We will seriously need this once he will be on
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Yes, we will and waiting for something spetial from other guys too
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|11 December 2010 at 4:54pm by -Mrinalini-
||Gurmeet-Dhrshti/Geet-Maan Appreciation thread||
Author: SunShine_A Replies: 1200 Views: 74986
|22 April 2010 at 4:05pm by -Lana
Daily Discusion Thread
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|06 April 2010 at 1:12pm by -Lana
Take a look at the hotties with beard...
Tellybuzz gets chatty with Drashti Dhami!
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Deviant Art
My film I've been working on for quite awhile. (since beginning of school year)
It doesn't seem like much for such a long time to work on it but then again I only have my animation class for an hour a day.
Plus I had OTHER assignments to focus on WHILE working on this one.
So I pushed my film aside to finish all my assignments so I could focus on it, once I finished the assignments it was half the year over. then I was finally able to focus on my film.
I finished it quite quickly too.
If I had more time I would of added a LOT more things and fixed up a lot of things that bother me here.
About the film.
The film begins the way it ends.
It's about a girl who is forsaken from others, she doesn't understand things the way other normal people do.
She also has a strange power that is shunned.
But a nice girl tries to befriend her.
She shows her kindness which the forsaken seems to respond to.
The nice girl tries to show forsaken her own friends but forsaken becomes quite jealous at this act.
She doesn't understand it's okay to have multiple friends and she wanted the nice girl to herself so she does an unspeakable act.
After the girl pushes her and runs off.
Forsaken is left alone once again.
I wish I could of inked it outlined it and showed you how look, I'll do a few digital pictures of them to show you guys~
Art, Animation and concept © by =bag-chan / Christina Brockus
Character designs © by =bag-chan and ~Ukarifirelover
|Sta.sh Submit


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| 5/26/2015 9:14 am
Perfect gift!by Nikki
My friend had been talking about these stones for ages and I was so happy when I found them on IWA. He loved them. We tried them out and now I see why he wanted them. They keep your drink chilled without watering it down. It's a really neat product.


text:
mozaBook expands teaching tools with its versatile and innovative illustration, animation and presentation possibilities. Users can create exercise books and digital textbooks, which can be enriched with interactive content (3D models, educational videos, sound files, images). More than 70 thematic applications help to arouse students' interest and help acquiring knowledge. The software is available for students, teachers and schools.
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Small 35½" x 25½" outside dimensions, dogs up to 40 lbs. Medium 40" x 26½" outside dimensions, dogs 40-60 lbs. Large 50" x 37" outside dimensions, dogs 60-120 lbs.
Overall: 4.4 / 5 based on 16 reviews
13 of 16 reviewers would recommend this product to a friend.
Not working out..... -
By: Molly5 from Hermosa Beach, CA
Sadly my dog gets close enough to sniff the bed and for some reason will not go near it. I have tried everthying to get her to even sit on the bed. No go. Disappointed. Something doesn't smell right.
Awesome dog bed -
By: KrisLabLover from Leesburg, VA
My 9 year old lab was starting to have some joint issues so I bought her this bed to help ease her discomfort. She loves it and sleeps in it any chance she gets. My kids were jealous-they said the dog has a better bed than they do!
Happy Dogs, Superb High Quality Bed -
By: SarahT from Chicago, IL
Our beloved 10-yr-old Shih Tzus (approx 9 lbs each) adore sleeping with us on our king-size Tempur-Pedic bed. During the day, however, they often lounge on one of many dog beds/pillows (soft, pillowy fabric & stuffing) throughout our home. However, there is a bed in the foyer which is their favorite (so they can watch for Mom, Dad & the beloved dog nanny). Recently, I began to notice that our Lola seemed to be a bit sore & needed to stretch her back legs much more after coming out of this bed after a long nap...so my antennae went up thinking arthritis may be starting given her age. Our vet confirmed that isn't the case yet but also noted the curled up position Lola favored in this small bed may not be helping. So, having discovered that Orvis sold the only REAL Tempur-Pedic bed, I ordered one immediately. Our babies love it - especially our long little Lola. She stretches out to sleep & I have not seen her momentarily favoring an uncomfortable limb post-nap since this new bed arrived! We love our little ones like family and since my husband & I love & gain relief from our own Tempur-pedic bed, how could we not provide this comfort for our "maturing" furry family members 24/7? Truly, I was impressed by the thickness of the Tempur-Pedic material & the "Small" size is ideal for our two dogs (they can both stretch out fully yet still feel cozy). While I selfishly wish the fabric options extended to lovely damask prints to match my decor (we got the mocha which is quite nice), I honestly would have purchased this bed even if it only came in hideous neon green polka dots simply because it makes our beloved dogs so happy & truly comfortable :) Thank you for providing such a high quality product for dogs, Orvis, and thereby acknowledging what important family members they are to so many happy households.
Awesome Purchase -
By: mlb2304 from Stafford, VA
I bought this dog bed about 2 years ago when my Dalmatian was a puppy. In anticipation that my puppy would be a very large dog one day, I ordered the largest size possible. At first at about 8-10 weeks he didn't like to sleep on it because it was so big (I think), but eventually as he grew I put it in his very large crate and he loves it. Because I've been training him with it, this bed is great because you can remove the outside cover to wash it in case of any accidents and there is thin plastic layer to protect the memory foam; contributing to the durability of the product. Also, I just called Orvis Customer Service, (TWO YEARS LATER) and asked how I would go about replacing the protective liner between the soft fabric outside, and the memory foam and they sent me a complimentary replacement! I was so excited that they did this for free and I didn't even ask. Orvis products are definitely the way to go. It's a quality product with a SERIOUS 100% satisfaction guaranteed. I'm a very happy camper!(:
love it. -
By: beagL
my 12 yr old hound just loves this bed... he won't leave it many times even for his favorite treat.. just wondering how they make that yellow lab pose in the same exact sleeping postion on all the different colored choices for this bed?
Best Bed -
By: Bedavies from Virginia
Best bed on the market for elderly or dog with arthritis.....hands down!!!
Bed too Hard -
By: mon28 from Chicago, IL
The bed looks nice however, my dog refused to sleep on it because it was too hard and typically he likes to snuggle himself in. He was unable to do so.
Nice heavy memory foam but... -
By: SebyCat from SC
I wanted to give this bed five stars, but I can't because the bolster is very understuffed. The foundation memory foam is very high quality and my dogs love it. However, the bolster is not firm enough/ doesn't have enough fill and so when my dogs lean on it it collapses over. Other than that I love the color and the soft fur, and my dogs seem to be enjoying laying on it. I got the large size and my Brittany and my Boxer mix share it.
excellent bed -
By: Bingosmom from Baton Rouge, LA
We ordered this bed for My 15 y/o Jack Russell & 6 y/o Boston Terrier. My JRT's arthritis & back pain had become severe. This bed's tempupedic mattress gives great joint support & keeps him warm. Now he rest well, the pain is reduced which decreased the need for as much pain medication. However, I do not recommend the faux fur. It sheds like crazy. We've have had them for 6 months & are ordering replacement covers. The beds are very expensive. Now I'm spending more $$ on replacement covers.
best bed for elderly golden retriever -
By: jebber from houston
This is the most high quality bed I have ever purchased. It is perfect for my elderly, 65 lb golden retriever. Though quite expensive, it is worth knowing that she is comfortable while fighting cancer and I would get it for future dogs. It is the first bed that she took to immediately. Usually, it takes a few weeks. The butternut color was backordered, so I went ahead with the chocolate color. It was fine, but I finally received the butternut color and it is blends in much better with home furnishings. I first got the medium and she could "snuggly" fit in it, but I exchanged for the large. It's more than enough room (see pic). I wish there was a size in between. The cover seems more durable and looks better than the one-piece memory foam bed. One suggestion is to have a handle on it so that it can be moved easily. Overall, you won't be disappointed!


text:
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text:
Defense Management:
DOD's Conference Policy Is Generally Consistent with OMB's Requirements
GAO-14-150: Published: Jan 21, 2014. Publicly Released: Jan 21, 2014.
Additional Materials:
- Highlights Page:
- Full Report:
- Accessible Text:
(202) 512-5741
Office of Public Affairs
(202) 512-4800
What GAO Found
The Department of Defense's (DOD) September 2012 policy on conferences and its November 2013 update are generally consistent with the requirements established by the Office of Management and Budget (OMB) in May 2012. The key elements of OMB's May 2012 requirements for agencies are the prohibition of conferences with costs in excess of $500,000 unless the agency head signs a waiver, establishment of a Deputy Secretary-level review process for conferences with estimated costs in excess of $100,000, and public reporting annually on the costs of these conferences. DOD adopted a tiered approval structure for the senior-level approval of waivers and all conference-related costs. DOD's policy, which cites the department's size and complexity, places the approval authority for conference waivers and for conferences costing less than $500,000 at lower levels than called for by OMB. For example, OMB requires that waivers approving conferences with costs in excess of $500,000 be signed by the head of an agency, while DOD's policy delegates this authority to 23 senior leaders throughout the department. DOD's policy is more expansive as it requires senior-level review and pre-approval of all conference-related costs, compared to OMB's requirement for senior-level review of conferences only when the estimated costs exceed $100,000. DOD's policy also fully addresses OMB's requirement to publicly report conference costs annually and adds a requirement for quarterly internal reporting of conference costs.
In implementing DOD's September 2012 policy (the existing policy when GAO conducted the majority of its review), DOD components--including the military departments--have taken various approaches to reviewing and approving conference requests, all of which are consistent with the policy. For example, DOD's policy allows senior officials within each component to delegate certain approval authority to lower-level officials for DOD-hosted conferences costing $500,000 or less and non-DOD hosted conferences costing $100,000 or less, but the components have delegated approval authority to different degrees. A majority of the 563 conference requests that GAO reviewed addressed and documented key elements consistent with DOD and component-level guidance. In requests that were missing documentation of one or more key elements, GAO found that a specific element was not missing from a significant number of requests. While the components' implementation of the conference review and approval process has generally been consistent with DOD's policy, some officials within the components and military service commands have identified concerns, particularly with the lengthy approval process. The officials explained that requests to attend conferences have to pass through multiple offices and individuals, sometimes taking several months to be approved. In particular, officials raised questions about the efficiency of reviews for requests to attend conferences that incurred no cost or a low cost (under $20,000) to DOD, which at the time of GAO's review went through the same process as higher-cost conferences. Almost 94 percent of the 405 requests to attend non-DOD hosted conferences that GAO reviewed were for conferences with no cost or a low cost to DOD. DOD in November 2013 updated its policy to state that approval is not required for conferences incurring no cost for DOD. However, a DOD official involved in writing DOD's conference policy stated that components still have the option to review conferences with no cost to DOD to facilitate senior leaders' visibility over conference attendance by personnel within their component.
Why GAO Did This Study
DOD hosts conferences and sends its personnel to external conferences for training, professional development, and continuing education. However, concerns about executive agencies' spending on conferences prompted OMB in 2012 to direct agencies to establish policies and practices for conference hosting and attendance. DOD issued its policy in September 2012 to improve oversight of conference costs and updated it in November 2013, citing lessons learned from implementing the September 2012 policy, among other things.
The conference report accompanying the National Defense Authorization Act for Fiscal Year 2013 mandated that GAO review DOD's oversight and management of conferences. This report assesses (1) the extent to which DOD's conference policy is consistent with OMB's conference requirements and (2) how DOD components have implemented DOD's conference policy. GAO assessed DOD's 2012 conference policy and 2013 update against OMB requirements and reviewed components' implementation of the policy. GAO analyzed responses to a questionnaire completed by officials from 72 components and military commands and reviewed 563 requests for conferences planned for the second and third quarters of fiscal year 2013.
GAO is not making recommendations in this report. In written comments, DOD concurred with GAO's findings and noted that it remains committed to balancing conference spending oversight with the benefits of hosting and allowing personnel to attend conferences.
For more information, contact Johana Ayers at (202) 512-5741 or email@example.com.
Oct 1, 2015
Ford Class Aircraft Carrier:Poor Outcomes Are the Predictable Consequences of the Prevalent Acquisition CultureGAO-16-84T: Published: Oct 1, 2015. Publicly Released: Oct 1, 2015.
Tricare Pharmacy Pilot:Improved Monitoring Needed with Expansion of Pilot RequirementsGAO-15-768: Published: Sep 30, 2015. Publicly Released: Oct 1, 2015.
Sep 30, 2015
DOD Contract Services:Improvements Made to Planning and Implementation of Fiscal ControlsGAO-15-780: Published: Sep 30, 2015. Publicly Released: Sep 30, 2015.
Sep 28, 2015
DOD Financial Management:Continued Actions Needed to Address Congressional Committee Panel RecommendationsGAO-15-463: Published: Sep 28, 2015. Publicly Released: Sep 28, 2015.
Sep 21, 2015
- Department of Defense's Waiver of Competitive Prototyping Requirement for the Air Force's F-15 Eagle Passive/Active Warning and Survivability System (EPAWSS) ProgramGAO-15-800R: Published: Sep 21, 2015. Publicly Released: Sep 21, 2015.
Sep 17, 2015
Prepositioned Stocks:Marine Corps Needs to Improve Cost Estimate Reliability and Oversight of Inventory Systems for Equipment in NorwayGAO-15-651: Published: Sep 17, 2015. Publicly Released: Sep 17, 2015.
Sep 9, 2015
Defense Infrastructure:In-Kind Construction and Renovation Projects Initiated by DOD during Fiscal Year 2013GAO-15-649: Published: Sep 9, 2015. Publicly Released: Sep 9, 2015.
Military Compensation:DOD Needs More Complete and Consistent Data to Assess the Costs and Policies of Relocating PersonnelGAO-15-713: Published: Sep 9, 2015. Publicly Released: Sep 9, 2015.
Sep 3, 2015
Military Personnel:Additional Steps Are Needed to Strengthen DOD's Oversight of Ethics and Professionalism IssuesGAO-15-711: Published: Sep 3, 2015. Publicly Released: Sep 3, 2015.
Aug 27, 2015
Weapon System Acquisitions:Opportunities Exist to Improve the Department of Defense's Portfolio ManagementGAO-15-466: Published: Aug 27, 2015. Publicly Released: Aug 27, 2015.
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Page 1
JOURNAL OF VIROLOGY, Sept. 2003, p. 10099–10105
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Vol. 77, No. 18
Combinations of Two Capsid Regions Controlling Canine Host Range
Determine Canine Transferrin Receptor Binding by Canine and
Feline Parvoviruses
Karsten Hueffer,1Lakshman Govindasamy,2Mavis Agbandje-McKenna,2and Colin R. Parrish1*
James A. Baker Institute, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University,
Ithaca, New York 14853,1and Department of Biochemistry and Molecular Biology, Center for Structural Biology, The
Brain Institute, College of Medicine, University of Florida, Gainesville, Florida 32610-02452
Received 10 March 2003/Accepted 23 June 2003
Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline
transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into
FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed canine
cell infection, although neither change alone altered either property. In CPV the reciprocal changes of VP2
residue 93 or 323 to the FPV sequences individually resulted in modest reductions in infectivity for canine cells.
Changing both residues in CPV to the FPV amino acids blocked the canine cell infection, but that virus was
still able to bind the canine TfR at low levels. This shows that both CPV-specific changes control canine TfR
binding but that binding is not always sufficient to mediate infection.
Canine parvovirus (CPV) emerged in the late 1970s as the
cause of a new disease in dogs and is now prevalent in dogs
worldwide. CPV was most likely derived as a host range variant
of the long-known feline panleukopenia virus (FPV), which
infects cats and some other carnivores, but not dogs (11, 18).
FPV became adapted to dogs through a series of steps, the first
being the emergence of the ancestor of the CPV viruses, which
gave rise to a variant (designated CPV type 2) which spread
worldwide during 1978 (30). However, by 1980 the CPV type 2
strain had been replaced worldwide by a variant strain desig-
nated CPV type 2a, which has remained prevalent in dogs with
only a small number of additional changes (19, 30). Each of
these evolutionary steps involved alterations in the capsid,
which changed both its antigenic and host range properties (19,
Parvoviruses have 25-nm-diameter T?1 icosahedral capsids
which are assembled from 60 copies of a combination of the
overlapping VP1 and VP2 proteins (31). The VP1 and VP2
structures contain an eight-stranded antiparallel ?-barrel, with
four large loops inserted between some of the ? strands mak-
ing up much of the viral surface (31). Features of the capsid
surface include cylinders around the fivefold axes, depressions
(the dimples) spanning the twofold axes of symmetry, and
22-A ˚-high raised regions (the threefold spikes) at the threefold
axes of symmetry (1, 31, 33).
Residues in three regions of the capsid surface control the
ability of CPV to infect dog cells. Two of those were seen as
differences between FPV isolates and CPV type 2, where the
changes in FPV of VP2 residue Lys 93 to Asn and Asp 323 to
Asn allowed the virus to infect dog cells (6, 9). In a CPV
background, changing either residue 93 or 323 to the FPV
residue reduced the ability of the virus to infect dog cells (6).
The side chain of residue 93 is exposed on the surface of the
capsid on the top of loop 1 (1, 31), and in FPV Lys 93 forms
two hydrogen bonds with backbone oxygen atoms of residues
225 and 227 of loop 2, while those bonds are not formed by the
Asn in CPV (1, 31, and L. Govindasamy, K. Hueffer, C. R.
Parrish, and M. Agbandje-McKenna, submitted for publica-
tion) (Fig. 1). VP2 residue 323 is separated from residue 93 by
20 A ˚, and the structure near that position is controlled in part
by the binding of up to three divalent ions (25 and L. Govin-
dasamy et al., submitted). A third region controlling infectivity
for dog cells is within a ridge on the side of the threefold spike
(the shoulder), and that contains three differences between
CPV type 2 and FPV (VP2 residues 80, 564, and 568) which
are not exposed on the surface of the capsid (13, 17). The
shoulder region also acquired three additional and surface-
exposed differences during the evolution to the CPV type 2a
strain (19). In addition, some changes in that region can elim-
inate canine cell infection of CPV (13, 17).
CPV and FPV both bind to the feline transferrin receptor
(TfR) and use that receptor to infect feline cells (16), and the
host range of CPV for canine cells is determined by the specific
ability of that virus to bind the canine TfR (11). Binding to the
canine TfR was lost in a host range mutant of CPV which had
a single substitution within the shoulder region of the threefold
spike (Gly 299 to Glu), but whether the other residues con-
trolling host range also affect canine TfR binding and its use
for infection is not known (11). Here we examined the specific
* Corresponding author. Mailing address: James A. Baker Institute
for Animal Health, Department of Microbiology and Immunology,
College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Phone: (607) 256-5649. Fax: (607) 256-5608. E-mail: crp3@cornell
Page 2
roles of residues 93 and 323 in controlling the binding of the
canine TfR and the effects on infection of canine cells.
To determine the functions of residues 93 and 323, we re-
placed VP2 residue 93 and neighboring residues on an adja-
cent loop with alternative amino acids (Fig. 1 and 2). Residue
323 was also changed alone or in combination with residue 93
so as to explore the functional interactions between those
residues (Fig. 1 and 2). Mutant viruses were prepared either by
site-directed mutagenesis of M13 uracilated single-stranded
DNA (12) or by using the Gene Editor protocol on the intact
infectious plasmid (Promega, Madison, Wis.). Some mutants
had previously been isolated as antibody neutralization escape
mutants (28).
When mutant genomes were tested for viability and host
range, it was apparent that considerable variation was allowed
in the structure of the CPV capsid near residue 93 without
affecting feline cell infection. All the viruses retained feline
TfR binding and feline cell infection (Fig. 2). Most CPV mu-
tants with single substitutions also retained the ability to infect
dog cells, although many showed lower titers in those cells than
in feline cells (Fig. 2B). In that assay, NLFK or A72 cells in
96-well plates were inoculated with 10-fold dilutions of virus,
FIG. 1. Structures of CPV and FPV within the areas examined in this study. (A) Atomic model of the structure of the CPV capsid in the vicinity
of VP2 residue 93. Labels indicate the VP2 residues that were substituted in these studies. Loop 1 is shown between VP2 residues 91 and 95, while
loop 2 is shown between residues 222 and 229. (B) Atomic model of FPV around residue 93 in the same orientation as in panel A. Labels indicate
the VP2 residues that were substituted during these studies. Hydrogen bonds between residue 93 and the backbone oxygen atoms of residues 225
and 227 are indicated by green lines. (C) Residues mutated in these studies shown on a roadmap of one asymmetric unit of the CPV capsid (24),
showing surface-exposed residues examined in this study. The number following the VP2 residue indicates the relationship of the VP2 molecule
containing the residue relative to the reference VP2 monomer in the standard orientation (2?, twofold; 3?, threefold; 5?, fivefold) (1). Residues
that were changed in CPV are marked in blue, those changed in FPV are marked in green, and those changed in both viruses are colored in red.
Residues 299 and 300, which were not altered in these studies but which also affect canine host range, are shown in brown. Other surface-exposed
residues are not shown here. The position of residue 323 in a neighboring asymmetric unit is indicated by the dashed lines.
Page 3
were incubated for 1 h, and then were incubated for a further
2 days in growth medium, after which the cells were fixed and
stained for viral antigen (17). The ratio between the titers
obtained for each virus in dog and cat cells measured the
efficiency of canine cell infection (Fig. 2B). Wild-type CPV
infected both NLFK and A72 cells to similar titers, but all CPV
mutants with single changes of residue 93 infected dog cells at
10- to 100-fold lower titers than were seen with the feline cells
(Fig. 2B). CPV with a single change of residue 323 to Asp
(CPV-N323D) also showed a 10-fold reduction in canine cell
infection, but the double mutant CPV-N93K/N323D was re-
stricted by 10?4, similar to the case of FPV (Fig. 2B).
FPV was highly restricted in dog cells, infecting only to 10?5
the titer seen in cat cells. However, FPV-K93N/D323N in-
fected canine cells to levels only 10-fold lower than levels for
CPV, but changing either residue 93 or 323 alone to Asn
resulted in no increase in FPV infectivity for canine cells (Fig.
2B). Replacing residue 93 in FPV with Arg instead of Lys
along with the 323 change to Asn (FPV-K93R/D323N) re-
sulted in a 250-fold increase in canine cell infectivity compared
to that for wild-type FPV (Fig. 2B).
Infectivities for NLFK and A72 cells were also examined by
testing for replicative-form (RF) DNA production after inoc-
ulation with CPV and FPV mutants containing reciprocal sub-
stitutions of residues 93 and 323 or with alternative changes of
residue 93 (CPV-N93D, CPV-N93R, FPV K93R, and FPV-
K93R/D323N). A72 or NLFK cells seeded at 104cells per cm2
in 9.6-cm2cultures were inoculated with a 50% tissue culture
infective dose (TCID50) of each virus of 0.5 per cell (as deter-
mined in NLFK cells), and then low-molecular-weight DNA
was recovered 2 days later according to a modification of the
method of Hirt (8, 17). After electrophoresis in a 1% agarose
gel containing 1 ?g of ethidium bromide/ml, the DNA was
blotted to a membrane and was probed with a32P-labeled DNA
sequence from the CPV genome. All mutants were infected at
similar levels in NLFK cells, while in A72 cells CPV-N93K,
CPV-N93D, CPV-N93R, and CPV-N323D showed reduced
levels of both infection and RF DNA production compared to
those of wild-type CPV, but CPV-N93K/N323D did not infect
to a detectable level (Fig. 2C). FPV-K93N/D323N infected
more efficiently than FPV-K93R/D323N but did so at lower
levels than CPV, and little or no infection was observed for
wild-type FPV, FPV-K93N, FPV-D323N, or FPV-K93R (Fig.
2C). Although the results of the TCID50and Southern blots
were closely correlated, differences in the assays occurred for
CPV-N93D, which showed more DNA in the canine cell cul-
tures than might be predicted from the TCID50assay. This is
likely explained by the fact that the TCID50detects only the
initial cell infection, while the RF DNA would measure mul-
tiple rounds of replication for the viruses that can infect the
In earlier studies using plaque titrations, a greater decrease
in canine cell infection was observed for single-point mutants
in a CPV background, although the results of DNA analysis
were similar to those observed here (6). The TCID50assay
used here is considerably more sensitive than the plaque assay
for detecting infections by viruses in cells that are relatively
resistant to the virus, as multiple rounds of infection of cells
would be required to form a detectable plaque (6).
The antigenic properties of the mutant viruses were tested
FIG. 2. Mutants prepared in these studies and their relative titers
in feline and canine cells. (A) The genetic background (CPV or FPV)
of each virus is indicated, as well as the changes(s) introduced into the
VP2 sequence. The single-letter code for amino acids was used, with
the letter before the number indicating the amino acid of the wild-type
virus and that following indicating the change(s) introduced. (B) Rel-
ative infectivity of each virus for canine cells shown as the ratio of the
TCID50titer of the virus stock in A72 cells to that in NLFK cells. Error
bars show one standard deviation of the mean for at least three inde-
pendent experiments. For the CPV-P229A mutant, data from one
(ratio, 2.8 log10) of seven independent experiments was excluded from
the analysis. (C) Infection and replication in NLFK and A72 cells of
selected viruses examined by the production of viral RF and single-
stranded DNA (ss). Cells seeded in six-well plates were inoculated with
0.5 TCID50per cell and were incubated for 2 days, and then the
low-molecular-weight DNA was recovered, electrophoresed in an aga-
rose gel, transferred to a membrane, and detected with a32P-labeled
DNA probe. The samples from NLFK cells were exposed for half the
exposure of the A72 cell-derived samples.
Page 4
using monoclonal antibodies (MAb) in a hemagglutination
inhibition (HI) assay (20, 21, 28). Most mutated viruses showed
reduced binding to several MAb that recognize antigenic site
A, which includes the regions around residue 93 (28) (Fig. 3).
An Asn at residue 93 creates an epitope recognized by all the
CPV-specific MAb tested (MAb 14, 7, 13, and 2A9), and sub-
stitution of residue 93 with any of several other amino acids
reduced or eliminated that epitope (Fig. 3). Antibodies recog-
nizing antigenic site A were clearly subdivided into two groups:
the CPV-specific antibodies that were affected by changes of
residue 93 from Asn to most other amino acids and those that
were not affected by changes of residue 93 but which were
affected by changes of residues 222 and 224 (Fig. 3). These
mutants show that the wild-type CPV structure was not re-
quired for the virus to infect canine cells, as many mutant
viruses showing reduced or no reactivity with CPV-specific
MAb still infected canine cells, albeit with reduced efficiency
compared to that of the wild type (Fig. 2 and 3). Changing
residue 323 alone did not alter the reactivity with any of the
MAb tested, and none of the mutants showed altered reactivity
with MAb 8, which recognizes antigenic site B (Fig. 3).
Infection of canine cells partially correlated with the degree
of virus binding and uptake into those cells (Fig. 4). For the
binding assays, cells seeded at 5 ? 104cells per cm2in 10-cm2
dishes were incubated for 1 h at 37°C with a 10-?g/ml concen-
tration of purified empty capsids of the indicated viruses (Fig.
4) (11). After being washed twice in cold Hanks buffered saline
solution without Mg2?or Ca2?(HBSS), cells were detached
with 1 mM EDTA in HBSS for 10 min at 4°C and then were
fixed for 10 min at 22°C with 4% paraformaldehyde. Cell-
associated virus was detected with MAb 8 conjugated to Cy2
(Amersham Biosciences, Piscataway, N.J.) in phosphate-buff-
ered saline with 0.5% (wt/vol) bovine serum albumin and 0.5%
(vol/vol) Triton X-100. Cells were analyzed by using a FACS-
calibur flow cytometer and Cell Quest software (Becton Dick-
inson, San Jose, Calif.). All viruses tested bound efficiently to
the feline NLFK cells, although FPV, FPV mutants, and CPV-
N93D and CPV-N93R all showed two- to threefold reduced
binding levels compared to those for CPV (Fig. 4A).
Levels of virus binding to canine Cf2Th cells were more
variable than those for binding to feline cells (Fig. 4B), and
similar results were obtained for A72 cell binding of the same
capsids (data not shown). CPV showed the strongest canine
cell binding, while mutants of CPV with residue 93 or 323
replaced bound at lower levels, some at close to background
levels (Fig. 4). The correlation between binding and infectivity
of dog cells was most clearly seen for FPV and its mutants,
where FPV-K93N/D323N bound and infected while wild-type
FPV and the mutants FPV-K93N and FPV-K93R/D323N did
not bind or infect those cells (Fig. 2 and 4B).
In previous studies viruses labeled with35S or3H were in-
cubated with cells at 4°C, and no significant differences in
binding to canine cells were seen between the closely related
mink enteritis virus and CPV or between a host range mutant
of CPV and wild-type CPV (10, 17). We examined the binding
of the viruses to feline and canine cells at 37 and 4°C and found
that at 4°C the CPV capsids bound to canine cells at levels
close to background, similar to the binding seen for FPV cap-
sids or for no capsids (Fig. 4C). At 4°C the binding to NLFK
cells showed moderate decreases of three- to fourfold for CPV
and 1.5- to 3.5-fold for FPV (Fig. 4C). This shows that binding
is temperature dependent and that temperature directly influ-
ences the association between the capsids and the cell surface
or the receptor(s).
We have shown that the specificity of capsid binding to the
feline and canine TfRs correlates with the host range of the
viruses and the susceptibility of the host cells (11). The binding
of mutant capsids to the canine TfR was therefore tested on
TRVb cells expressing the canine TfR from a plasmid after
transfection, and the cells were simultaneously incubated with
iron-loaded canine transferrin conjugated to Cy5 (11). All vi-
ruses tested efficiently bound the feline TfR expressed on
TRVb cells (results not shown) (11, 16). All of the viruses that
infected canine cells showed efficient binding to the expressed
canine TfR at 37°C (Fig. 5). Mutants CPV-N93K, CPV-N93D,
and CPV-N93R all bound the canine TfR, but they did so at
levels lower than were seen for the wild-type virus, as higher
expression levels of the TfR on the cells (as seen by transferrin
binding) were necessary before virus binding was detected
(Fig. 5A). Although CPV-N93K/N323D was not able to infect
the canine cells, it still bound the canine TfR, although at levels
that were lower than those for the single mutants (Fig. 5A).
Of the FPV mutants, FPV-K93N and FPV-D323N did not
show detectable binding to the canine TfR, while FPV-K93N/
D323N, which was able to infect canine cells, bound at levels
that were similar to those of CPV-N93K/N323D (Fig. 5B). It
FIG. 3. Antigenic analysis of the viruses examined by using MAb in
an HI assay. Eight hemagglutinin units of virus were incubated for 1 h
with twofold dilutions of monoclonal antibodies in the assay, and then
erythrocytes were added. The results are shown as the titer of the
antibodies for each virus relative to that of wild-type CPV. Filled
boxes, mutant HI at 10 to 100% wild-type titer; cross-hatched boxes,
less than 10% but greater than 1% of the wild-type titer; open boxes,
less than 1% of the wild-type titer.
Page 5
therefore appears that canine TfR binding is required for in-
fection of canine cells, that additional host-specific steps are
required for successful infection, and that infection is influ-
enced by residues that act in combination with VP2 residues 93
and 323. In previous studies of CPV binding to the canine TfR,
binding to the canine TfR expressed on TRVb cells led to only
low levels of infection by the CPV type 2 strain, while those
cells were more efficiently infected by the CPV type 2b strain
VP2 residues 93 and 323 are separated by 20 A ˚in the capsid
structure and there is no direct association between them, but
both sites are required for successful capsid interactions with
the canine TfR. Other sites in the CPV capsid are also involved
in controlling canine TfR binding and canine cell infection.
This was seen for CPV-N93K/N323D, which bound the canine
TfR despite the FPV-derived Lys and Asp at residues 93 and
323, indicating that other structural differences in that virus
must allow binding to the canine TfR in the presence of those
changes. However, that virus-receptor interaction was not suf-
ficient for infection. Mutant CPV-G299E does not infect ca-
nine cells or bind the canine TfR (11, 17), and residue 299 is
?30 A ˚from both residues 93 and 323 (Fig. 1C), indicating that
FIG. 4. Binding of CPV, FPV, or mutant virus capsids to feline or canine cells. Cells were incubated with 10 ?g of virus/ml for 1 h at 37°C, and
then after cell detachment, fixation, and permeabilization the capsid antigen was detected by antibody staining and flow cytometry. (A) Binding
to feline cells of CPV capsids and mutants in a CPV background (upper panel) or FPV capsids and mutants in an FPV background (lower panel).
(B) Binding to canine cells of CPV capsid and mutants in a CPV background (upper panel) or FPV capsids and mutants in an FPV background
(lower panel). (C) Effect of temperature on binding of CPV or FPV capsids to feline or canine cells. Cells were incubated with 10 ?g of virus/ml
for 1 h at 4°C or at 37°C, and then after detachment, fixation, and permeabilization viral antigen was detected by antibody staining and flow cytometry.


text:
Orange Bowl: Clemson vs. Ohio State—Everything You Need to Know
Tajh Boyd and Braxton Miller square off in this matchup of two of the nation's top offenses.
Tajh Boyd and Braxton Miller square off in this matchup of two of the nation's top offenses.
Magnet Man may sound like a superhero villain, but it's actually a real person.
Ugly Christmas sweaters are almost as much a part of the holiday season as chestnut roasting on an open fire, so why not turn the trend into a few extra bucks in your pocket?
Josh McCown passed for 348 yards and four touchdowns, and the Bears routed the Dallas Cowboys, 45-28, in frigid Chicago on Monday night.
Every college has unique traditions, but this one is worth shouting about.
There aren't many games left this season, but the ones on tap are huge. Here are the big questions as we go into championship weekend:
Love him or hate him, Tom Brady is a star. So, when one man got the chance to get his picture taken with the New England Patriots quarterback, he went for it.
That decision cost him his job.
There's one commercial people won't be talking about after the Super Bowl.
The NFL reportedly refused to air a commercial during the big game for an outfit called Daniel Defense because it is for guns...
The holidays can bring out people's stranger sides, and Thanksgiving is no exception.
UPDATE 3:15 p.m. EST: The lockdown and shelter-in-place orders have been lifted, according to the Yale alert system. Police have been going door-to-door on campus searching for the gunman, though authorities will not confirm whether it may have been a hoax.
Thanksgiving, of course, is a time to pause and reflect about all the good in our lives, but what exactly are we thankful for this year?
Black Friday can be crazy, which may be why some folks are going to extremes to make sure they don't miss out on the deals.


text:
9ct white gold quarter carat diamond solitaire earrings£110
Product code: 4571789
Return within 30 days for full refund or exchange.Delivery Info Returns Info
These gorgeous stud diamond earrings weigh a quarter of a carat and have a three-claw 9ct white gold setting to allow for maximum light, which means maximum sparkle.
An enduring pair of earrings you'll love wearing forever.
- Birthstone
- April
- Diamond
- 1/4 carat
- Material
- 9ct White Gold
- Stone setting
- Claw
- Stone shape
- Brilliant (Round)
- Stone style
- Solitaire
- Stone type
- Diamond
- Width
- 0.3 cm
Delivery & Returns Information
Standard delivery costs £3 for orders under £100 and is free when you spend £100. You can have your order delivered Next Day delivery to any address in the UK for £5 or purchase online and have your order delivered to store for free. View all delivery information.Return within 30 days for full refund or exchange.


text:
RCBS Bench Mounted Primer Pocket Swager
Product #: 319662 RCBS #: 9474 UPC #: 076683094742
- Information
- Videos
The RCBS Bench Mounted Primer Pocket Swager is a dedicated bench mounted tool that makes the removal of primer pocket crimps a simple task. This rugged tool requires 35 percent less force than other tools on the market to complete the same task. A hardened steel rod supports the case from the inside allowing the case to float for perfect alignment with the hardened steel swager head. All a reloader has to do is rotate the case down into position and with a pull and push of the handle, the job is done.
Technical Information
Function: Primer Pocket Swager, remove the primer pocket crimp that is present on most military brass
Works with: Removes the crimp from both large and small primer pockets
Accessories: Comes with hardened steel swaging heads and rods for both large and small primer pockets
Hardened steel rod supports
Hardened steel cam
Die cast aluminum base
foam padded grip handle
35% less force to remove primer pocket crimps than other comparable tools
Works with cartridge cases from 22 caliber and larger
Warranty: RCBS Limited Lifetime Warranty
Technical Information
Function: Primer Pocket Swager, remove the primer pocket crimp that is present on most military brass
Works with: Removes the crimp from both large and small primer pockets
Accessories: Comes with hardened steel swaging heads and rods for both large and small primer pockets
Warranty: RCBS Limited Lifetime Warranty
Delivery Information
The total weight of the product, including the product's packaging as it sits on our shelf. Since some products ship directly from the Manufacturer, their weight may be unavailable. Because of variations in the manufacturing process, similar products may have differing weights. Unless the product is designated as "Ships Alone", Shipping Weight does not include the shipping box or packing materials associated with your order.
Product ships from MidwayUSA, separate from any other products on your order due to its size. Products that are Shipped Alone incur separate "Per Order" and "Per Pound" shipping charges.
Product is regulated by the US Department of Transportation (DOT) and cannot be shipped via USPS or air shipping methods. DOT-Regulated products must ship UPS Ground.
Product is restricted by shipping carriers and cannot be shipped to certain locations or via certain shipping methods.
Additional shipping charge for HazMat products. Carriers charge extra to ship packages with products they consider hazardous, such as reloading powder and primers.
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Select Local Merchants
Tempero Do BrasilRead MoreSeafood stew and skewered meats are served fresh from the grill at Tempero Do Brasil, a Brazilian spot that will not disappoint. None of the fare at Tempero Do Brasil is low-fat, so you'll have to put the diet aside for a visit here. Order a bottle for the table if you like — Tempero Do Brasil has a full bar stocked with the best wine, beer, and more. Have a few picky young eaters in the family? Not a problem at Tempero Do Brasil, where the food and ambience are perfect for family dining. Make the most of the warm summer months by dining outdoors in Tempero Do Brasil's beautiful outdoor seating area. Casual dining at its best, Tempero Do Brasil customers are free to enjoy themselves in jeans and a T-shirt. Tempero Do Brasil can also cater your next party; call today for details. Carry-out is also available for those who prefer to enjoy Tempero Do Brasil's cooking from the comfort of their own home. Guests take to street parking at Tempero Do Brasil's University Way NE spot. The menu at Tempero Do Brasil is reasonably priced, with most items costing less than $30.5628 University Way NESeattle, WA US
Novilhos Brazilian SteakhouseRead MoreWhen night falls over southern Brazil, groups of gauchos gather around flickering fire pits and celebrate the end of another day by slowly grilling meat over the open flames. Novilhos Brazilian Steakhouse aims to capture the spirit of these traditional meals by hosting all-you-can-eat churrasco feasts that Zagat scored as "very good to excellent." The chefs roast up more than a dozen cuts of meat, including filet mignon, bacon-wrapped chicken, lamb chops, and pork sausages. Servers dressed as gauchos parade these freshly grilled skewers throughout the dining room, stopping at diners' seats and offering to carve them slices tableside. In between these protein-laden servings, guests can visit the salad bar and load their plates up with more than 60 different side dishes, such as fresh-cut vegetables and imported cheeses.901 Fairview Ave. NSeattle, WA US
Samba Brazilian RestaurantRead MoreAt Samba Brazilian Restaurant, guests can expect one of the more inexpensive meals in Seattle. The nice atmosphere and reasonable prices allow customers to focus on what matters: the fantastic food. Streetwear attire is acceptable, so feel free to come as you are. Also, though the prices are considered to be lower than average, you aren't going to sacrifice any quality. There really is something for everyone, with gluten-free options, as well as low-fat and vegetarian items on the robust menu. When you put it all together, Samba Brazilian is a tasty choice when you're in the mood for Latin cuisine, and want to break away from the standard Mexican fare.9400 Roosevelt Way NESeattle, WA US
Jimmy macRead MoreJimmy mac in Federal Way is a well-known steakhouse, offering a relaxed atmosphere with terrific steak and tasty burgers. It's a local favorite for customers that want great food. No specific attire is required, so feel free to dress casually and comfortably. Also, it's often reported that the background noise can get a little loud here. It's been tabbed as a nice option for both families with children and large groups. WiFi's available if you want to link your device up, and in addition to its convenient take-out menu, the restaurant even provides catering for events around town. Or, if you just want to stop by for a beverage, the restaurant has a pretty broad selection at its bar. Specializing in both lunch and dinner, Jimmy mac is definitely an appetizing option to quell your next munchies craving. The restaurant is easily reachable via public transit, and visitors who drive have access to a private lot nearby.34902 Pacific Hwy SFederal Way, WA US
Salty's at Redondo BeachRead MoreFresh fare can be found at Salty's at Redondo Beach, where diners seek to sample every seafood dish on the menu. Life is all about choices, and they are not limited here with plenty of gluten-free and low-fat dishes. Unwind with a glass of wine or cocktail with your meal — Salty's at Redondo Beach has a wonderful selection of drinks to accompany your dinner. Salty's at Redondo Beach is a terrific spot for families to gather with its kid-friendly ambience and menu. Salty's at Redondo Beach will be able to accommodate your large party. You can tote your laptop here to take advantage of the free wifi. Sit back, relax, and enjoy the beautiful weather during your meal at Salty's at Redondo Beach. Drift away from stuffy dress-code conventions and dine in comfort at Salty's at Redondo Beach. Or, take your food to go. Catering services are also available. There are many generous parking options near Salty's at Redondo Beach, including street, valet and garage. Fancy snacks do come at a higher price, but wow are they delicious. Early risers and night owls alike can enjoy Salty's at Redondo Beach since it offers breakfast, lunch, and dinner.28201 Redondo Beach Dr SDes Moines, WA US
Angelo'sRead MoreAngelo's puts a fresh spin on traditional Italian cuisine. Don't expect to find any low-fat fare on Angelo's' menu — you'll need to be prepared to indulge a bit. Don't go thirsty during dinner! Angelo's also offers a splendid drink list featuring wine, beer, and more. The perfect place to take the kids, dining out at Angelo's won't cost you a sitter. Angelo's features some of the most affordable happy hour deals in town. Get connected at lightning fast speeds with Angelo's' complimentary wifi. Skip long waits and head to Angelo's with your large group for easy seating. Be sure to call for a reservation if the restaurant is part of your weekend plans — it can get crowded on Fridays and Saturdays. Call Angelo's for catering if you have a big event coming up. You can also grab your food to go. Find a close parking spot on the street or in a parking lot near Angelo's. For those who prefer to travel by bike, Angelo's is a great option due to its generous bike parking options. Frugal foodies will especially love the prices at Angelo's, with a meal typically hovering under the $15 mark.601 SW 153rd StBurien, WA US


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Under new maritime laws all vessels and boats using Fiji waters have to abide by the rules and regulations under the Marine Transport Decree, reports FBC
Turkey launched the 140-meter amphibious ship "Bayraktar," the first of two vessels that are part of a domestic Landing Ship, Tank (LST) project, reports Local media.
The Coast Guard urges the public Thursday to use extreme caution and to prepare for the onset of heavy weather, extreme sea conditions, storm surge and high
The U.S. Coast Guard now believes that the missing cargo ship El Faro sank after sailing into the path of Hurricane Joaquin in the Bahamas, a spokesman said on Monday.
The Coast Guard medically evacuated a 46-year-old man from a 738-foot container ship approximately 6 miles south of Santa Rosa Island, Sunday.
At 10:01 a.
Search and rescue teams on Sunday located debris which appeared to belong to the cargo ship El Faro that went missing in the eye of Hurricane Joaquin with 33 mostly American crew members aboard,


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Beaumont, California Pet Friendly Hotels & Lodging
United States » California » Beaumont
Looking for a hotel in Beaumont, CA that you can bring your pet to? We made sure that each one of the hotels below are pet friendly, so you can enjoy your trip with your pet by your side! Once you have found a pet friendly hotel, you can book your stay instantly from our website or call 866-212-1803 to speak with a pet travel agent.
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625 E. Fifth St. - Beaumont, CA (Map)
Americas Best Value Inn allows pets to accompany their people at their pet friendly accommodation.
Overview | Pet Policy | Map
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1265 E 6th St - Beaumont, CA (Map)
Pet guests are welcome at pet friendly Rodeway Inn Beaumont. Each room can have no more than 1 pet which can be up to 50 pounds. The pet fee is $10 per pet, per night.
Overview | Pet Policy | Map


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Foursquare © 2015 Lovingly made in NYC & SF
2901 Bayview Ave. (in Bayview Village)
2901 Bayview Avenue, Unit #C105 (at Sheppard Ave E)
"Like us on facebook!! Gap bayview village"
Banana Republic
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Re: September Weather Hong Kong
No problem, just good to know that the pilots are use to the September weather. Actually I think September is a great time to enjoy Hong Kong. So don't worry, bring a umbrella.
cheers tommy x
Re: September Weather Hong Kong
Usually, there are not many flights affected by typhoons in September. As far as I recall it...there was one day last year in September where flights had been delayed or canceled due to typhoons. The HK Airport is quite efficient and pilots, crews and airport staff are aware of these weather changes. In addition, most typhoons hit Taiwan first in the past and then arrived weaker in HK and Mainland China bringing lots of rain.
Re: September Weather Hong Kong
Thank you both for your prompt responses! This certainly puts my mind at rest, I can now relax and look forward to our trip to HK in September. Kelly
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Is it common to be nervous about a miscarriage? If so how do you deal with it? is there ways to help prevent a miscarriage? I am 7 weeks along and after my first child i had a miscarriage at 11 weeks and am very nervous now that i'm pregnant again..
nervous about miscarriage
Comments (3)
I am sorry your first pregnancy ended in miscarriage.
There is nothing that will make you stop worrying. I lost my first baby, so I know the worry won't stop. Keeping a journal helps. I bought "The Pregnancy Journal" early in my (successful) pregnancy and it helped. Talking with others that have lost babies helps as well.
You should be considered high risk by your OB/GYN and get more ultrasounds, etc. This will help catch problems early, if there are any. Otherwise I don't know what would help prevent miscarriage. Was the cause of the first miscarriage found and treated? Getting enough sleep, eating well and light exercise, such as walking, are good for pregnant women. Also, since you're high risk, it would be a good idea to go on "pelvic rest", meaning no sex until you get past that "danger point" and there's no longer a worry about miscarriage.
Good luck to you.
Once you've experienced a miscarriage, yes, it's natural to be scared that you might have another one. I had a miscarriage at age 20. I got pregnant with my son in 2010 and was scared until after I hit 12 weeks. They say if you get past 12 weeks, you're in the clear. Just think positive and try not to stress about it. You have to keep yourself busy for the next few weeks. GL!
Jackson Carl. Nov. 4th, 2010. 9 lbs 9 oz, 22.5 inches long.
Honestly I was freaked out about it with all my Pregnancys... even before I ever lost one. I've had 2 miscarriages now and I found a site on facebook that has helped a lot www.facebook.com/pages/Angel-babies... ... check it out if you want. I found it was a lot more helpful to me than anything else. and is helping me be hopeful in this pregnancy as well.


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The Xcalibur EEratic Shad 4.5" Jerkbait features erratic, lifelike action and a self-adjusting weighting/rattle system that is great for long casts. #6 Tx3 in-line treble hooks. 1/2 oz. 4-1/2" long.
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Important Messages!!
E-bills will be uploaded on October 6th. Payment will be due on
October 27th.
Non-payment of this bill by October 27th will result in a hold being placed on your account. Holds prevent receipt of transcripts and/or registration for future classes.
Billing and Registration Schedule
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The forum observed recently that the two respondents were government officers and they performed their duties as per law.
A business forum for opening up new opportunities for trade and commerce between India and the Middle East will be held in November.
The AIEF shall be focusing on opportunities in infrastructure, alternative energy, digital India, tourism and finance, the forum said in a statement.
A consumer forum here has held that it cannot order Thane Police Commissioner and RTO to fine autorickshaw union for its strike in the city in April this year.
The World Forum of Fisher Peoples (WFFP) today highlighted the need for a moratorium on arrest of fishermen whether it was in Indian, Pakistani or Sri Lankan waters.
Unlike other angel networks in the country, Keiretsu does not charge companies a percentage of the money raised or demand an equity from them.
China will host a major conference on the Silk Road initiative to boost international trade and cooperation on infrastructure projects with Central, South and West Asian countries.
The forum - initiated and supported by embassies and consulates of Brazil, Russia, India, China and South Africa - will be held on September 9 at Dubai.


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Welcome to the Town of Wawayanda. Our Town is located in Orange County NY, with a population of 7,266 as recorded by the 2010 census. The Town is in the western part of the county, south of Middletown. The Town of Wawayanda was established in 1849 from the Town of Minisink. It had been first settled after the American Revolution.
This site has been created and designed to provide you with convenient access to public information concerning our community, its people, its government and services.
We hope you find our site helpful. It is our goal to provide timely and useful information to you as well as another medium for you to communicate with your local government. Feel free to browse our site. Thank you and WELCOME!!
PLEASE TAKE NOTICE that the Local Development Corporation will be holding a Meeting at 4:00 P.M. on Monday, October 5, 2015 at the Town Hall,
80 Ridgebury Hill Road, Slate Hill, NY. The purpose of this meeting is to approve the minutes of the last meeting and address any other business that may come before the Board.
The Town of Wawayanda will make every effort to assure that the meeting is accessible to persons with disabilities. Anyone requiring special assistance and/or
reasonable accommodations should contact the Town Clerk.
Dated: September 30, 2015
Mark Specthrie - Secretary
Town of Wawayanda
Local Development Corporation
The pre-filing reports are public documents, available for download on the company's website at http://www.millenniumpipeline.com/valley_lateral_project.html
A paper copy is also available for review at the Wawayanda Town Hall.
The Assessors Office is in the process of updating our records to ensure current inventory of all properties.
The Assessor and Data Collector will be taking exterior photos for the next several months.
If anyone has any concerns please contact the Assessor's Office at 845-355-5700, Option 9
Effective on October 14, 2009, all open burning is prohibited in New York with several exceptions including the following:
In towns with a total population less than 20,000, you may burn tree limbs with attached leaves. The limbs must be less than 6 inches in diameter and 8 feet in length (also referred to as brush). However, this is not allowed from March 16 through May 14 due to the increased risk of wildfires.
Does NY State Owe You Money? Check the office of unclaimed funds
$39 Million Dollars in unclaimed funds is owed to Orange County Residents. See if you are one of them.


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Below is a list of all parts for the KitchenAid Range KESC308LSS0. To find the repair part you need, you can browse the list below or you can narrow your choices by searching for a part by name or description.
This part goes over top of the light bulb.
This hinge is located on each side of the oven door.
This broil element is found on the roof of the oven.
This is a 1500 watt surface element.
This cleaning cream is intended for use with smooth top ranges and cook tops with either black or white glass ceramic tops, stainless steel, or chromed surfaces. This cleaning cream is non-abrasive and will remove most cleaning soils.
This screw is sold individually.
10-32 hex - Sold Individually - Appliance originally takes 3 of this item.
Use this scraper for your ceramic/glass cooktop. It has a retractable blade and is an all-purpose tool for cooktops, mirrors, windows and tile. Use it to also remove paint, paper...
Sold individually.
This heating element is found under the base of the oven.
This temperature sensor detects the temperature within the oven cavity.
All brand logos are trademarks of their respective owners.
The PartSelect logo is a Registered Trademark of Atlantic Laundry Centres, Ltd.
Copyright © 1999-2015 , Eldis Group Partnership. All rights reserved.


text: The Brian Lehrer Show Edit this Published in The Brian Lehrer Show Reclaiming Kipling Listen 15 min Queue Download this audio Want to embed this player? Use the code below. More info. <iframe frameborder="0" scrolling="no" height="130" width="100%" src="https://www.wnyc.org/widgets/ondemand_player/wnyc/#file=/audio/json/29360/&share=1"></iframe> Share on Facebook Share on Twitter Email a Friend Bob Holman, poet and proprietor of the Bowery Poetry Club, comments on how Rod Blagojevich has taken to quoting Rudyard Kipling. Question of the Day: What work would you quote if you were embroiled in scandal? Comment below!


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As of Thursday, September 26, 2013
© Copyright 2015 Gwinnett Daily Post
Inequality For All
2 1/2 out of 4 stars
A graduate of both Yale and Dartmouth, a Rhodes Scholar and author of 14 best-selling books, Robert Reich — whether you agree with his politics or not — can rightfully be referred to as a well-respected economist. After serving in the Clinton Administration as Secretary of Labor, Reich became and remains a fixture on both the late night and Sunday morning political talk show circuits and when not a TV talking head he teaches classes on economics at Cal Berkeley.
Unlike most former presidential cabinet members, Reich is a natural in front of the camera. He is affable, eminently likeable, pokes fun at himself and regularly makes light of the affliction (Fairbanks Disease) that stunted his growth (he stands at 4' 10" — yet has a towering presence). If anyone was ever qualified to write, narrate and appear in a movie about economics it is Reich.
In manner not unlike that of Al Gore's "An Inconvenient Truth" and most movies by Michael Moore, Reich — despite his proclamations to the contrary — brings a marked liberal bias to "Inequality For All." This isn't such a bad thing per se; the movies by Gore and Moore are very entertaining — and the same can be said for Reich's film. It is never boring or dry, imparts a wealth of valuable information in a highly audience-friendly manner and — at less than 90 minutes long — never overstays its' welcome.
For the first half of the movie Reich — largely avoiding egghead, "inside baseball" jargon — presents his argument with workmanlike efficiency by employing stylish charts and graphs, stock video/newsreels and hard-to-argue-with facts and figures supporting his case: the ever-widening chasm between the haves and have-nots is a problem.
Reich's preferred visual is a graphic that is a profile view of a suspension bridge and he drives home that the highs, lows and trends of several economic indicators of the last century match each other almost identically. Whenever there is economic distress — like during the Great Depression or right now — the disparity in median income is significant. When everything was hunky dory — the time between the end of World War II and the mid-1970s — the difference in income percentage was far closer (and "fair"); management/ownership made only a little more than their employees, despite taking all of the risk.
Reich's reasons for these fluctuations are many and varied but the thread that ties all of them together is the same: the rich are greedy and the poor are victims. While presenting many reasons for the former, Reich essentially ignores the latter. He cites President Ronald Reagan's squashing of the Air Traffic Controllers Union as the first domino, then the proliferation of industry lobbyists on the (bipartisan?) Capitol Hill and ultimately the inflated salaries of company CEOs.
Reich touches briefly on the rise of industrial prowess in Asia, the replacement of assembly line workers in the U.S. with computer-based automation and alternately slams/extols the business model of Amazon. Reich fails to acknowledge the union-controlled U.S. public school system that has produced a sub-par workforce for decades and greed on the part of the middle-class-hopeful masses that bought homes they couldn't afford and opened lines of credit they weren't qualified for in the mid-2000s.
Reich and director Jacob Kornbluth deserve major kudos for including a scene taking place at a meeting at a western U.S. power plant where Reich was trying to prod the employees into unionizing.
Respectfully arguing against the union proposal, a skilled laborer states he feels he's being paid more than he deserves and has no problem with the company officers making way more than him. He adds that if he was smart and had gotten smarter by going to college, he'd be trying to get their jobs. Hmmm.
The filmmakers also get bonus points for including video/sound bites from wealthy people (including Warren Buffet) who find the current tax code (where they pay only 10 to 15 percent) ludicrous and they're right. Most of this could easily be eliminated with a flat or consumption tax — a subject Reich never broaches. Though he never uses the actual words, Reich's intent is to suggest an American society based on the quasi-socialist "redistribution of wealth" dictum. People with drive, dedication, vision and a strong work ethic should supplement the layabout lifestyles of those who do the absolute bare minimum (or less) in order to just get by — while of course always owning of the latest smart phone.
This brings to mind a number of quotes from someone you might have heard of. "Most folks are as happy as they make up their minds to be." "Always bear in mind that your own resolution to succeed is more important than any other." "My great concern is not whether you failed but if you are content with your failure." "That some achieve great success is proof to all that others can achieve it as well." All of those quotes were spoken or written by President Abraham Lincoln. (Radius-TWC)
More like this story
- Greater Atlanta Christian's Wood finds right fit at Dartmouth ( February 5, 2014 )
- RAMPELL: Limousine liberalism's good works ( July 23, 2014 )
- PARKER: The real meaning of words ( January 8, 2014 )
- Obama warns divided Congress that he will act alone ( January 29, 2014 )
- Ivy League, Army the impressive destinations for GAC seniors ( February 5, 2014 )


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Companies demand return on their social-media investment. How to take a deliberate approach:
Build it wisely. Imagine a cocktail hour where everyone's in one room but no one's talking.
That's what happens when companies gather followers on Facebook (NASDAQ:FB) or similar platforms without bringing a purpose to the party.
"Counting the number of followers, that's not strategic," Mikolaj Piskorski, author of "A Social Strategy," told IBD.
Think it through. Give people a reason to show up.
"You have to put effort into the structure," Piskorski said.
Cisco Systems (NASDAQ:CSCO) did just that when it built virtual communities around its training programs.
Enhance the interaction. Imagine two people engrossed in a discussion at a cocktail party — until you pull up a chair and try to sell them something. "You are interrupting the conversation. You are not welcome," Piskorski said.
Beware of pushing an unwanted promotional message. Make sure you're offering something of value, like a hint on how to better use the product they're discussing.
Follow the audience. Peter Friedman, author of a forthcoming book on social media, managed Apple's (NASDAQ:AAPL) first online community nearly three decades ago.
Now CEO of LiveWorld, a social-content marketing firm, Friedman has watched virtual interaction between companies and consumers become ubiquitous. People expect to connect online. "Their eyeballs are there. Their hearts and minds are there," he said. "You have to have dedicated resources on it."
Hold on loosely. The key way that social-media marketing differs from conventional advertising: "Your customers are driving it," Friedman said.
That means that conversations can go off the firm's desired track. "You will have crises," he said. "Have a social-media crisis-management plan in place before it happens."
Play it out. Friedman's team engages in social-media war gaming by imagining troubling scenarios, working out logical responses and monitoring chatter to pick up rumblings of trouble. "Identify these things early," he said. "Have lots of responses worked out."
A thoughtful yet speedy reply lends credibility. "Even a day later can be too long," he said.
Gather backup. "You don't make friends in the middle of a crisis," Friedman said. Cultivate fans — also called brand advocates — before trouble erupts.
Those enthusiasts might come to your defense if the online conversation takes a negative turn.
Get more views. Think those YouTube videos go viral by accident? Virool CEO Alexander Debelov says firms are taking a more concerted approach to building buzz.
His company helps everyone from up-and-coming musicians to emerging brands draw the masses to their video content.
A first step before uploading a clip? "Reach out to tastemakers related to the video topic and give them a preview," Debelov said.
Tesla Motors (NASDAQ:TSLA) CEO Elon Musk says Apple (NASDAQ:AAPL) isn't a serious electric threat, telling German newspaper Handelsblatt that Apple hires from Tesla are failures. "They have hired people we've fired," Musk said. "We always jokingly call Apple the 'Tesla Graveyard.' If you don't make ...
Major stock ETFs reversed to close higher Thursday as newly released Federal Reserve minutes revealed policy makers' anxieties about the global growth outlook. SPDR S&P 500 (ARCA:SPY), tracking the broad market, finished up 0.9% in above-average volume. The $172.69 billion ETF punched through ...
Retailers posted September same-store sales gains Thursday that inched past analysts' views, as consumers stepped up spending at the end of the back-to-school season. Sales at stores that were open for at least a year rose 0.8% from a year earlier, said Ken Perkins, president of Retail Metrics. ...
Stereo headphones and tablets are expected to be the top technology gifts this holiday shopping season, according to a survey by the Consumer Electronics Association. So expect to see a lot of Beats headphones and tablets from Apple (NASDAQ:AAPL) and Amazon.com (NASDAQ:AMZN) being opened on ...
Stocks ticked up after the afternoon release of the Fed's Sept. 16-17 meeting minutes, closing near the high of the day. It was the sixth time in seven sessions the Nasdaq has finished near the top of its daily trading range. The Nasdaq rose 0.4%; the S&P 500 gained 0.9% and the Dow Jones ...
Milton Friedman constantly illustrated how free markets work. He would hold up a yellow wooden pencil and begin: "There's not a single person in the world who can make this pencil." The 20th century's apostle of capitalism would then explain: No single person on earth has the expertise, ability and ...
Field Marshal Alan Brooke was British Prime Minister Winston Churchill's chief military advisor, and together they helped lead the Allies to victory. But they nearly killed each other before it was over. "They disagreed loudly and frequently," Christopher Hamner, author of "Enduring Battle," told ...
For Miller Huggins, the choice of a baseball career was a matter of listening to his heart. Oh, and future President William Howard Taft. Huggins was the first legendary manager of the New York Yankees, who'd had seven skippers in 10 years before his 1918 arrival. Owner Jacob Ruppert credited ...
It's hard to imagine how William "Buffalo Bill" Cody overcame the logistical problems of transporting his "Wild West" show. After becoming a legendary Army scout during America's Western expansion, Cody wanted to preserve the memory of the vanishing frontier life. Trains carrying his 500 cast ...
Chances are you've seen Michael Strahan over the past few days. With his "Live! With Kelly and Michael" syndicated morning TV talk show, regular on-air contributions to ABC pillar "Good Morning America," football analyst gig Sundays on Fox, men's clothing line, production company and new book, the ...
Want more from your broker? See which stock brokers our annual survey reveals as the top five and how they rank in areas most important to investors. Check out all of them now!
Investor's Business Daily provides exclusive stock lists, investing data, stock market research, education and the latest financial and business news to help investors make more money in the stock market. All of IBD's products and features are based on the CAN SLIM® Investing System developed by IBD's Founder William J. O'Neil, who identified the seven common characteristics that winning stocks display before making huge price gains. Each letter of CAN SLIM represents one of those traits.


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All-Star Jam Session
Actor Joel Moore reacts to winning the Celebrity Slam Dunk on center... News PhotoActor,Adidas,Arts Culture and Entertainment,Basketball - Sport,Bestof,California,Celebrities,Center Court,City Of Los Angeles,Facial Expression,Joel David Moore,Los Angeles Convention Center,NBA Pro Basketball,Slam Dunk Contest,Sport,Topics,Topix,USA,Vertical,WinningPhotographer Joe MurphyCollection: National Basketball Association 2011 NBAELOS ANGELES, CA - FEBRUARY 20: Actor Joel Moore reacts to winning the Celebrity Slam Dunk on center court at Jam Session presented by Adidas during NBA All Star Weekend at the Los Angeles Convention Center on February 20, 2011 in Los Angeles, California. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and or using this photograph, User is consenting to the terms and conditions of the Getty Images License Agreement. Mandatory Copyright Notice: Copyright 2011 NBAE (Photo by Joe Murphy/NBAE via Getty Images)


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This Promotion is only for ordering the same item no.
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This offer is valid while stock last.
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This HA Eye Serum delivers intensive treatment for the delicate eye area. It helps to prevent and reduce fine lines, wrinkles and crow's feet, premature ageing, puffiness. Promote firmer, smoother and moisture eye contours and provides lifting and firming effects. The skin on the eye contours quickly nourished and hydrated. There is a visual plumping and improvement in the skin texture, giving back its youthful appearance
how to use it
Morning and evening, evenly apply on perfectly cleansed eye area by tapping gently with fingertips. Don't rub
Hyaluronic Acid,Ginseng Extract, Vitamins A, B1, C & E, Ginkgo Biloba, Horse Chestnut, Marine Collagen, Chamomile and Yeast Extract
suitable for
all skin type


text:
A gentleman, who's cool, calm and collected under pressure. A leader who has the ability to listen. A nice guy who happens to be single.
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46 • Alexandria, VA • Man
I'm looking for
- Women
- Ages 30–50
- Near me
- Who are single
- For new friends, long-term dating, short-term dating
My details
- Last online
- Today – 2:19pm
- Orientation
- Straight
- Ethnicity
- Black
- Height
- 6′ 2″ (1.88m)
- Body Type
- Fit
- Diet
- Mostly anything
- Smokes
- No
- Drinks
- Socially
- Drugs
- Never
- Religion
- Christianity, and somewhat serious about it
- Education
- Graduated from masters program
- Job
- Law
- Income
- Rather not say
- Status
- Single
- Offspring
- Has a kid
- Speaks
- English (Fluently)
My self-summary
Write a little about yourself. Just a paragraph will do.
What I'm doing with my life
Don't overthink this one; tell us what you're doing day-to-day.
Professionally - striving to make it happen
Personally - living stress and drama free
Personally - living stress and drama free
I'm really good at
Go on, brag a little (or a lot). We won't judge.
Being a father to my child
The first things people usually notice about me
I'm an empty essay… fill me out!
I'm a gentleman
Favorite books, movies, shows, music, and food
Help your potential matches find common interests.
TV Show - Finding your Roots
Book - currently reading, "Power Forward, My Presidential Education," by Reggie Love
Movies - Shawshank Redemption
music - depends on my mood
food - a nice ribeye, sushi and Korean Fried Chicken
Book - currently reading, "Power Forward, My Presidential Education," by Reggie Love
Movies - Shawshank Redemption
music - depends on my mood
food - a nice ribeye, sushi and Korean Fried Chicken
The six things I could never do without
Think outside the box. Sometimes the little things can say a lot.
good friends
a peace of mind
My man toys
good friends
a peace of mind
My man toys
I spend a lot of time thinking about
Global warming, lunch, or your next vacation… it's all fair game.
How to retire young
On a typical Friday night I am
Netflix and takeout, or getting your party on — how do you let loose?
Normally I'm relaxing after a long week, would love to have a companion to chill with.
The most private thing I'm willing to admit
I'm an empty essay… fill me out!
I can't swim
You should message me if
Offer a few tips to help matches win you over.
If you're seeking to meet a really nice guy.
FYI, my kid graduated from college (no small children or baby mama drama)
FYI, my kid graduated from college (no small children or baby mama drama)
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text:
- Reports (7)
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text: There is nothing but jaw dropping beauty, calm tranquil surroundings, excellent welcome the moment you cross the threshold. I could keep on going and going, there simply are no negatives! I mean that. If I had to really really try and find one, no I still can't. Michael the manager, his team, awesome. A real credit. Thank you all.