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Hampton Inn & Suites Buffalo Downtown
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How has this hotel rated in the past?
- Suites
- Continental Breakfast
- Shuttle bus service
- Free High-Speed Internet
- Swimming pool
- Restaurant
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Historical Traveler Reviews Hampton Inn & Suites Buffalo Downtown
Best Option in Downtown Buffalo
If you are staying in Downtown Buffalo for business the Hampton Inn and Suites on Delaware and Chipewa is the best option. The hotel is new and well cared for. The rooms are clean and comfortable. The staff in the lobby are friendly and helpful. They offer a free buffet breakfast which is decent. The Hampton Inn is located in a small area of cafes, restaurants and bars which makes it about the only place in downtown Buffalo where you can go out after 5:30pm and not be the only person on the street. The hotel is about 4 blocks from the train that goes up and down Main Street.
Excellent hotel
Stayed here on business for one night and was favorably impressed. Staff was friendly and helpful, parking is free and so was internet access. Clean room and spacious as well. In a less than spectacular area of downtown but I don't think downtown Buffalo is impressive overall. Security seems tight and the place really was convenient for business downtown.
Favorably impressed
Wow! A surprisingly upscale, downtown look for a Hampton Inn! Rooms were spacious, high-ceilinged, and ours had a gas fireplace! Walking distance to bars, coffeehouses, restaurants, theater. Staff was friendly and accomodating (we asked to switch from 2 doubles to a king and they did so immediately and then called a little while later to see if we liked our room). Breakfast was so-so. Only downside was that there were noisy guests coming in during the wee hours after drinking on Chippewa St. Don't know if this is a frequent or unusual occurrence at this hotel. At any rate, I would recommend this hotel - good bargain and gives a nice impression of Buffalo.
Hampton Inn & Suites is a very Beautifull Place
I recently stayed at the Hampton Inn & Suites in Downtown Buffalo located right in the Entertainment District. The hotel was great, furnished very stylish, friendly staff, and our room had it's own patio, kitchen area, hot tub and a view of Lake Erie. At night time (Friday Night) my wife and I walked around the area checking out some of the dance clubs and restaurants. The next morning after a hang over we had helped ourselfs to the Complimentary Breakfast buffet and coffee. Warm eggs and bagels..yumm.
I would definitely book another room at this hotel on my next visit to Buffalo.
We stayed here on our honeymoon night. My fiance stayed there the night before in a different room, and you'd think with it being his wedding day, they could have been more accommodating. NO! He asked for either a late check out or early check in but they was told "we don't do that". When he said we would both be staying there that night and asked for room service to leave some champagne for our first night together they "didn't do that either" and NO ROOM SERVICE??? NO Restaurant?? For a downtown hotel? You would have thought the "City of Good Neighbors" would have given the poor guy a break on his wedding day, but they made him have to go to management to finally get a late check out. We should have stayed at Salvatores Italian Gardens in the Honeymoon suite with free breakfast!
An up to date renovation that's a great deal
A coworker and I coincidentally visited Buffalo within a month of each other and couldn't have had two hotel experiences more different. C. stayed at the Hyatt, a luxury brand. She found the hotel quite disappointing. The Hampton Inn, however, provides an experience worth far more than the $110 corporate rate. Nicely appointed rooms, a great location downtown that's also a block from the beginning of the Elmwood corridor, a coin laundry, computer lounge, and across the street from a fine indie coffee shop, The Spot, as well as a Starbucks. It may be more expensive than a typical Hampton, but it's worth it.
Best Location for Fun
Just steps away from the "entertainment" street. If you're out to have some fun, you can't beat the location. The rooms were above average and the free breakfast more than acceptable.
One thing after another....
The desk clerk who processed our reservation was surly and offensive.
The fire alarm went off at 3:00 a.m. for no valid reason. While we were out of our room the next day, a bag of possessions went missing. It turned out to have been taken by a housekeeper. One item was recovered and I was reimbursed for the rest, but only after my persistent phone calls in the next few days while they slowly unravelled the matter.
When I arrived at the front desk to collect the money, one of the staff in attendance had his mouth full of food. Please come back, he said, as I left. Right!
Tough to beat for the price !!
With a student at Canisius college we've made many trips to Buffalo and have tried several places for overnight accomodations. This Hampton has become our #1 place to stay in Buffalo. It is in immaculate condition with friendly staff and excellent customer facilities including indoor pool and business center. We particularly enjoy it's central location making for easy access to a full range of dining and entertainment locations. We like the security of this Hampton which requires your room key for everything from parking to elevator use. Highly Recommended.
Outstanding hotel
Compared to the generally pathetic state of most Buffalo hotels (and just about every building downtown for that matter), the downtown Hampton Inn and Suites is off the charts. Brand new, large rooms, FREE on-site parking, large in-door swimming pool and sauna, free continental breakfast, secure facility (you can't anywhere without a key card, including the parking lot), beautiful lobby, halls and rooms. I can't believe I'm saying this about a Hampton Inn. Stayed at the Hyatt Regency downtown Buffalo the first night and it was one of the worst hotels I've EVER stayed at. Absolutely horrible. If you have the misfortune of being stuck in miserable, depressed, bankrupt downtown Buffalo, try the Hampton and you won't be disappointed. It's an oasis.
TripAdvisor Reviews Hampton Inn & Suites Buffalo Downtown
Travel Blogs from Buffalo
The wonders of the waterfalls
... bliddy Maydin, maaan!!"), all three of us sink into our seats and become even more mute, resolving to sit as far away from them on the bus as we can. Eventually they get the hint and once onboard start talking to other unsuspecting souls instead. I feel guilty for, ooh, seconds...
And so ...
Niagara Falls...just as beautiful as you'd expect
... we are on a bus all day to Salt Lake. We have been on here since 7.15am and it is now 4pm and we still have 2 hours more. But we get some rest stops so you can stretch so it has been fun, just a lot of bus travel but at least you see the country instead of the sky.
Hopefully I can get Boston done in the next few days but I am catching up here and there having just done Alaska for you so I know you will enjoy the pictures in this blog especially.
Love to you all,
Toni ...
... just to make sure that we could get the train to Albuquerque on Saturday, which we could, so we booked it, and then headed out as we still had a few hours to kill before our bus was due to come and pick us up.
We headed back down to the falls and took loads of pictures, we were going to do some of the activities but they are just too expensive and we didn't really have the time to do them properly either. The falls do ...


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Hourly - Entry Level ($) - Est. Time: More than 6 months, Less than 10 hrs/week - Posted
We are looking for a Umbraco Web Developer who can help maintain and keep our site functional and attractive. You will need to be organised so that the coding for site is clean and organised. Planned Projects for the site. - Making the site mobile responsive - Ensure that we have a good blogging framework - Create Supplier Membership Area to manage own travel deals - Fix up the way we work with images inside Umbraco CMS - Fix up XML feeds from partner travel sites Ongoing Expectations - Ensure we have solid backup plan - Code is SEO ready - Ensure packages and plugins are up to date Requirements - Good English Skills - Good Communication Skills - Problem and Solution Solving Skills - Be available for weekly video conference (Skype etc) If you think you would like to help us and fix up and make sure our site is humming! We want to hear from you.

subset: commoncrawl


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The ultimate frequent flyer: Air passenger who bought 'unlimited' American Airlines ticket 20 years ago clocks up 38 MILLION miles
- Jack Vroom has completed enough flights to circumnavigate the world 1,600 times
- American Airlines remove his 'unlimited' pass after realising it costs them $1m a year
An air passenger has staked a claim as the ultimate frequent flyer - after clocking up a staggering 38 million airmiles.
Jack Vroom made a long term investment in 1989 when he snapped up a lifetime, first class travel ticket for any global route from American Airlines for almost $500,000.
And the Texan air traveller has racked up a record amount of mileage for the two decades since thanks to his supposedly 'unlimited' air pass for himself and any companion.
Grounded: Jack Vroom has been denied his American Airlines unlimited pass after over 23 years
But after amassing enough air miles to circumnavigate the world 1,600 times, Mr Vroom has now been grounded - after American Airlines accused him of selling his companion tickets.
Mr Vroom, 67, paid around $350,000 plus interest for the ticket 23 years ago, and at times travelled the world four times a week as he cashed in on his investment.
He would regularly take friends and family on worldwide trips as his 'companion', and even helped fly home AIDS victims to see their families thanks to his extra seat.
Mr Vroom bought the AAirpass in 1989 while working as a consultant for mail order catalogues.
He already travelled alot by plane on business and bought the pass after hearing about it from a friend.
Mr Vroom estimates that there are only around 40 people in the world who currently own the AAirpass, which the airline stopped selling in 2004.
Premium access: Mr Vroom's pass allowed him first class travel with American Airlines on any global route
American Airlines removed Mr Vrooms AAirpass after making an accusation of fraud against the frequent flyer
When American Airlines calculated, however, that the AAirpass used by Mr Vroom and other frequent flyers was costing the airline around $1m a year, they grounded him over accusations of 'improper activity'.
Jack Vroom's 23 years as a holder of the AAirpass allowed him to make dozens of seemingly unnecessary journeys, all without spending a dime.
One flight saw him travel from the U.S. all the way to London just so he could visit a Lush cosmetics store on the King's Road.
He also flew to Washington D.C. to pick up his father-in-law so he could babysit for Vroom's children, explaining to a bemused passenger that it was 'cheaper than paying a local teenager'.
When his son was in college Mr Vroom would regularly fly to Maine every Saturday to watch him play football.
Other regular journeys saw him fly to Guadalajara, Mexico with a sheet of raw silver, where he would visit his favourite craftsmen and return with a bag full of newly-made belt buckles.
The incident in 2009 has bought to a halt years of air travel which Mr Vroom said was 'like a 9 to 5 job'.
Mr Vroom's staggering airmiles make George Clooney's, who clocks up a meagre 10 million miles in Up in the Air, look like a part-time flyer.
Mr Vroom travelled around the U.S., as well as Europe and other parts of the world, often staying at his destination for just a few hours before flying back again without spending anything.
He told CBS that during one flight he travelled to Milan just to pick up some exhaust pipes for a motorcycle.
While his son was in college in Maine, Mr Vroom flew 2,000 miles every Saturday to watch him play football.
His travel adventures have since ground to a halt following a flight back from London, where he had used his companion pass to fly his daughter's friend back to the U.S.
An American Airlines spokesperson said they still offer the lifetime pass, but have removed Mr Vrooms as they 'had issues' with him.
A spokesperson said: 'Our AAirpass program, including unrestricted, fixed-rate air travel to both domestic and international markets, is expected to remain unchanged.'
Small fry: George Clooney's character in Up in the Air clocks up a mere 10 million air miles - not a patch on Mr Vroom's 38 million
'In addition, all VIP AAirpass privileges, including complimentary Admirals Club membership, Priority AAcess privileges, companion travel benefits and AAdvantage mileage accrual are expected to remain intact during the Chapter 11 process.'
'As part of our ongoing security practices and normal business operations at American Airlines, we actively analyze all of our ticketing and program policies for any improper activity, including with our AAirpass accounts.'
'If we determine that any activity has violated our policies or is fraudulent in nature, including the non-fraudulent provisions that were included in these original contracts in question, we take the actions we deem appropriate.'
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Walder DJ, Walker EF, Lewine RJ. Cognitive functioning, cortisol release, and symptom severity in patients with schizophrenia. Biol Psychiatry 48: 1121-1132
ABSTRACT There is substantial evidence of dysregulation of cortisol secretion, hippocampal abnormalities, and memory deficits in schizophrenia and other psychotic disorders. Research also suggests that cortisol secretion augments dopaminergic activity, which may result in increased symptom expression in this clinical population.
We examined the relations among cortisol release, cognitive performance, and psychotic symptomatology. Subjects were 18 adults with schizophrenia or schizoaffective disorder, seven with a nonpsychotic psychiatric disorder, and 15 normal control subjects. Tests of memory and executive function were administered. Cortisol was assayed from multiple saliva samples.
Findings indicated the following: 1) patients with psychotic disorders scored below the comparison groups on the cognitive measures; 2) for the entire sample, cortisol levels were inversely correlated with performance on memory and frontal tasks; and 3) among patients, cortisol levels were positively correlated with ratings of positive, disorganized, and overall symptom severity, but not with negative symptoms.
The present results suggest that abnormalities in the hypothalamic-pituitary-adrenal axis and hippocampal systems play a role in observed cognitive deficits across populations. Among psychotic patients, elevated cortisol secretion is linked with greater symptom severity.
Full-textDOI: · Available from: Rich Lewine, Sep 26, 2015
Click to see the full-text of:
Article: Walder DJ, Walker EF, Lewine RJ. Cognitive functioning, cortisol release, and symptom severity in patients with schizophrenia. Biol Psychiatry 48: 1121-1132
75.72 KBSee full-text
- SourceAvailable from: Alfonso Gutierrez-Zotes
- "Among different biological factors, hormones may play an important role in the pathogenesis of cognitive impairment in psychotic disorders. Most studies have focused on cortisol (Walder et al., 2000), although others, such as thyroid hormones, are also important . Thyroid hormones play an important role in the differentiation and growth of a healthy human brain and consequently in cognition. "
Article: Free thyroxine levels are associated with cognitive abilities in subjects with early psychosis[Show abstract] [Hide abstract]
ABSTRACT: Subjects with a psychotic disorder show mild to moderate cognitive impairment, which is an important determinant of functional outcome. The underlying biological process of cognitive impairment in psychosis is unclear. We aimed to explore whether hypothalamic-pituitary-thyroid axis hormones or thyroid autoimmunity modulate cognitive functioning in subjects with early psychosis. We studied 70 patients with a psychotic disorder (<3years of illness) and a control group of 37 healthy subjects (HS). Plasma levels of thyroid-stimulating hormone (TSH), free thyroxine (FT4) and thyroid-peroxidase (TPO-Abs) and thyroglobulin antibodies (TG-Abs) were determined. Cognitive assessment was performed with the MATRICS Cognitive Consensus Cognitive Battery. We also explored the relationship between thyroid variables and cognition in three subgroups of psychotic patients: psychosis not otherwise specified, affective psychosis (bipolar disorder or schizoaffective disorder) and non-affective psychosis (schizophrenia or schizophreniphorm disorder). In patients with early psychosis, higher FT4 levels (but not TSH or thyroid antibodies) were associated with better cognitive performance in attention/vigilance and overall cognition. The relationship between FT4 levels and the attention/vigilance domain remained significant in a multivariate analysis after adjusting for education level, age, gender, substance use, and benzodiazepine and antipsychotic treatments. We did not find a significant association between FT4 and cognitive performance in HS. In the exploratory analysis by psychotic subtypes, subjects with affective psychosis had increased FT4 levels and better cognitive profile than those with non-affective psychosis. Our study suggests that FT4 levels are associated with cognitive abilities (attention/vigilance and overall cognition) in individuals with early psychosis. Copyright © 2015 Elsevier B.V. All rights reserved.Schizophrenia Research 05/2015; 166(1-3). DOI:10.1016/j.schres.2015.04.030 · 3.92 Impact Factor
- "This study found a strong negative correlation between dexamethasone abnormalities (dexamethasone non-suppressors) and cognitive performance in type II schizophrenics, implying a relationship between HPA axis hyperactivity and cognitive impairment. Furthermore, Walder et al. (61), evaluating 18 patients with chronic psychosis, 7 patients without psychosis, and 19 healthy controls, found that, in the entire sample, cortisol levels were negatively correlated with performance on memory and executive tasks. "
Article: A Systematic Review of Cognitive Function in First-Episode Psychosis, Including a Discussion on Childhood Trauma, Stress, and Inflammation[Show abstract] [Hide abstract]
ABSTRACT: Objective: To carry out a systematic review of the literature addressing cognitive functions in first-episode psychosis (FEP), divided into domains. Although this is not a full "cognitive-genetics-in-schizophrenia review," we will also include putative ideas of mechanism(s) behind these impairments, focusing on how early stress, and genetic vulnerability may moderate cognitive function in psychosis. Method: Relevant studies were identified via computer literature searches for research published up to and including January 2013, only case-control studies were included for the neurocognitive meta-analysis. Results: Patients with FEP present global cognitive impairment compared to healthy controls. The largest effect size was observed for verbal memory (Cohen's d effect size = 2.10), followed by executive function (effect size = 1.86), and general IQ (effect size = 1.71). However, effect sizes varied between studies. Conclusion: Cognitive impairment across domains, up to severe level based on Cohen's effect size, is present already in FEP studies. However, differences in levels of impairment are observed between studies, as well as within domains, indicating that further consolidation of cognitive impairment over the course of illness may be present. Cognitive abnormalities may be linked to a neurodevelopmental model including increased sensitivity to the negative effect of stress, as well as genetic vulnerability. More research on this field is needed.Frontiers in Psychiatry 01/2014; 4:182. DOI:10.3389/fpsyt.2013.00182
[Show abstract] [Hide abstract]
- "For example, basal cortisol levels are higher in patients with manic psychoses than in patients with schizophrenia (Christie et al., 1986); the post-mortem distribution of glucocorticoids receptors in the central nervous system is decreased in both patients with schizophrenia and those with bipolar disorder, but it differs according to anatomical site in the two diagnostic groups (Webster et al., 2002); and HPA axis responses to pharmacological challenges is different among different subtypes of schizophrenia (Duval et al., 2003). When examining populations with mixed diagnoses (schizophrenia and affective psychoses), two studies did not find significant associations between cortisol levels and specific symptoms (Pruessner et al., 2008; Garner et al., 2011) and one found an association with both positive symptoms and disorganization (Walder et al., 2000). Christie and colleagues compared subgroups of patients with different diagnoses of psychotic disorders, at various stages of the illness: in their study, cortisol levels correlated positively with the severity of positive symptoms irrespective of the diagnosis, but they also correlated with negative symptoms in schizophrenia, with depressive symptoms in depressive psychoses, and with excitement symptoms in manic psychoses (Christie et al., 1986). "
ABSTRACT: Abnormalities in hypothalamic-pituitary-adrenal (HPA) axis activity have been reported in patients with psychosis, but it is still unclear how these are related to the clinical symptomatology. Inconsistent findings have emerged from previous studies on the association between cortisol levels and clinical symptoms. Methodological and/or clinical factors, such as patients' diagnosis or illness phase, might partially account for these inconsistencies. The aim of this study was to investigate the association between HPA axis activity and clinical symptoms in first-episode psychosis, taking into account diagnosis and illness phase. Saliva samples were collected in 55 subjects with first-episode psychosis to assess the Cortisol Awakening Response (CAR) and diurnal cortisol levels (AUC-DAY). Severity of symptoms was assessed with the Positive and Negative Syndrome Scale (PANSS). Scores for subscales and symptom dimensions were used as predictors in multivariate analyses in different diagnostic subgroups and in clinically remitted patients. In addition, a systematic review of the literature on this topic was conducted. In subjects with schizophrenia (n=36), the CAR was predicted by the severity of positive symptoms (beta=0.47, p=0.04); in subjects with depressive psychoses (n=8) the CAR was predicted by excitement (beta=0.58, p=0.005), disorganization (beta=0.39, p=0.007) and depressive symptoms (beta=0.32, p=0.005). In patients with bipolar psychoses (n=11) AUC-DAY was predicted negatively by disorganization (beta=-2.82, p=0.009) and positively by excitement (beta=2.06, p=0.009) and positive symptoms (beta=1.28, p=0.02). In the sample in clinical remission (n=9), the CAR was associated with the severity of positive symptoms (beta=1.34, p=0.009) and, negatively, with excitement (beta=-1.05, p=0.04). The systematic review (on a total of 28 papers, including n=1022 patients), found that in patients with psychosis cortisol levels have been associated with the severity of multiple symptom dimensions. HPA axis activity is associated with the severity of multiple types of symptoms in first-episode psychosis. Patients' diagnosis and clinical phase partially influence these associations.Psychoneuroendocrinology 09/2011; 37(5):629-44. DOI:10.1016/j.psyneuen.2011.08.013 · 4.94 Impact Factor


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310 Views 1 Reply Latest reply: Feb 9, 2013 5:26 AM by jcorbin69993
jcorbin69993 Feb 9, 2013 5:26 AM
Re: File sharing - Before and after OS X Server installation in response to jcorbin69993 Solved
My solution to this was easy. I just used OS X server to manage back ups. Once I turned Time Machine on in server.app I could select the required disc and it created a back up folder on the drive. I was then able to select this folder/drivein Time Machine preferences as the backup disc.
If anyone else is as much as a novice as I am and wasn't getting any help from this forum, hopefully this will help you.


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Courtney Pine
4 of 6 portraits of Courtney Pine
© Trevor Leighton / National Portrait Gallery, London
Courtney Pine
by Trevor Leighton
bromide fibre print, 27 September 1990
11 7/8 in. x 9 in. (302 mm x 228 mm)
Given by Trevor Leighton, 1990
Photographs Collection
NPG x35749
Click on the links below to find out more:
Sitterback to top
- Courtney Pine (1964-), Saxophonist. Sitter in 6 portraits.
Artistback to top
- Trevor Leighton (1957-), Photographer. Artist associated with 248 portraits, Sitter in 3 portraits.
Subjects & Themesback to top
Tell us moreback to top
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The New England Patriots were poised to get the ball back in overtime after Jet's kicker Nick Folk missed a 56-yard field goal, but a penalty changed all of that and left the Patriots stunned and confused.
On the missed field goal, Patriot's defensive tackle Chris Jones was called for an unsportsmanlike penalty for pushing a teammate into the Jets line, giving the Jets a first down, and a few plays later the win when Folk hit a 42-yard field goal for a 30-27 victory.
Logan Mankins joined Michael Felger on PostGame live to discuss the bizarre ending to the game.
"That was a tough one," Mankins said. "It was great to see him miss it until you saw that flag on the ground. Hugely disappointing. I'm still not exactly sure what happened, so I'm going to have to wait to hear that one."
To try to make the game safer, pushing a teammate into the opponent's offensive line during a field goal attempt became a penalty this year.
"I'm aware of the rule, they just change that this year," Mankins said. "I guess if they saw that they saw that. Kind of ticky-tacky for the game-winning play there. It is what it is. They called it and it really hurt us."


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|Up a level
Ainsworth, J. D. and Carruthers, E. and Crouch, P. and Green, N. and O'Flaherty, M. and Sperrin, M. and Williams, R. and Asghar, Zahid and Capewell, S. and Buchan, I. E. (2011) IMPACT: a generic tool for modelling and simulating public health policy. Methods of Information in Medicine, 50 (5). pp. 454-463. ISSN 0026-1270
Leontidis, Georgios and Caliva, Francesco and Al-Diri, Bashir and Hunter, Andrew (2015) Study of the retinal vascular changes between the early stages of diabetes and first year of diabetic retinopathy. In: IOVS Association For Research in Vision & Ophthalmology, 2 - 7 May, 2015, Denver, USA.
Flynn, John (2009) Excel dataset for Chi-squared test, risk ratio and odds ratio. [Dataset] (Unpublished)


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Author has written 14 stories for D N Angel, Static Shock, Naruto, Ben 10, Harry Potter, South Park, and Twilight.
I can now be found on AO3, where things with a more explicit rating (Not that I plan to write any at the moment) can be found. It's also a quicker place to receive updates and contact me.
As for here... I actually don't write much these days, but anything I do write that would be allowed here will show up. I deleted my newer things out of sheer embarrassment. Of the things that are here, they were all written when I was a teenager, between 13 and 18 (In fact, the most recent fic was posted the day after I turned 18). I'm 22 now and a lot has changed. I'll be cleaning up the profile and deleting any stories I don't plan to work on.
I'm considering writing Suicide Watch again. I actually kind of liked it. We will see.
|General: All Categories


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|By Liz McMillan
Article Rating:
|February 16, 2014 11:00 AM EST
DevOps Summit Early Bird Savings here!
Development teams have a tendency to think that once they push the deploy button, the release is done. But with DevOps, it's quite the opposite now. More software changes, and more deploys, make every production release a new piece of code with new idiosyncrasies. This has made what has historically been a more static production environment now highly dynamic. Teams have responded by layering in a lot of new monitoring and performance technologies, which has caused even more information overload.
Continuous Deployment makes it easier to push code to production, and it has created an additional priority within the company - the need to provide Continuous Support. Continuous Support addresses the human side of how to harness your team in more effective ways, using new methodologies to adapt to a much different world.
In his session at 2nd DevOps Summit, Todd Vernon, CEO of VictorOps, will outline best practices for implementing - and sustaining - Continuous Support.
Explore DevOps Summit Sponsorship & Exhibit Opportunities !
Speaker Bio:
Todd H. Vernon Founded VictorOps Inc. in 2012 and serves as its Chief Executive Officer. Mr. Vernon has been Executive Vice President of Technology at Federated Media Publishing, Inc. since October 2011. Mr. Vernon Founded Lijit Networks, Inc. in 2006 and serves as its Chief Executive Officer. He serves as a Mentor at TechStars, LLC. Mr. Vernon served as the Product Architect and Lead Developer at Rogue Wave Software, where he was responsible for the design and development.
A Rock Star Faculty, Top Keynotes, Sessions, and Top Delegates!
DevOps Summit at Cloud Expo® 2014 New York, June 10-12, at the Javits Center in New York City, New York, will feature technical sessions from a rock star conference faculty and the leading Cloud industry players in the world.
The growth and success of Cloud Computing will be on display at the upcoming Cloud Expo® conference and exhibition in New York City, New York, June 10-12, 2014.
All main layers of the Cloud ecosystem will be represented at the 14th International Cloud Expo® - the infrastructure players, the platform providers, and those offering applications, and they'll all be here to speak, sponsor, exhibit and network.
Sponsorship Opportunities
for 2nd DevOps Summit
Sponsorship, exhibit, and keynote opportunities can be obtained from Carmen Gonzalez by email at events (at) sys-con.com, or by phone 201 802-3021.
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DevOps Summit delegates can pre-register for 2nd DevOps Summit with Early Bird Savings here.
About SYS-CON Media & Events
SYS-CON Media (www.sys-con.com) has since 1994 been connecting technology companies and customers through a comprehensive content stream at www.sys-con.com - featuring over forty focused subject areas, from Cloud Computing to Web Security - interwoven with market-leading full-scale conferences produced by SYS-CON Events. The company's internationally recognized brands include among others Cloud Expo® (www.CloudComputingExpo.com), Big Data Expo (http://BigDataExpo.net), Virtualization Conference & Expo (www.VirtualizationConference.com), DevOps Summit (http://DevOpsSummit.sys-con.com), WebRTC Summit (http://WebRTCSummit.net), Government IT Conference & Expo (www.GovITExpo.com), Cloud Computing Bootcamp (www.CloudComputingBootcamp.com), and UlitzerLive! New Media Conference & Expo (http://events.sys-con.com).
Cloud Expo® is a registered trademark of Cloud Expo, Inc.
The last decade was about virtual machines, but the next one is about containers. Containers enable a service to run on any host at any time. Traditional tools are starting to show cracks because they were not designed for this level of application portability. Now is the time to look at new ways to deploy and manage applications at scale. In his session at @DevOpsSummit, Brian "Redbeard" Harrington, a principal architect at CoreOS, will examine how CoreOS helps teams run in production. Attende...
Oct. 8, 2015 05:45 PM EDT Reads: 1,215
The buzz continues for cloud, data analytics and the Internet of Things (IoT) and their collective impact across all industries. But a new conversation is emerging - how do companies use industry disruption and technology enablers to lead in markets undergoing change, uncertainty and ambiguity? Organizations of all sizes need to evolve and transform, often under massive pressure, as industry lines blur and merge and traditional business models are assaulted and turned upside down. In this new da...
Oct. 8, 2015 05:30 PM EDT Reads: 221
The Internet of Things (IoT) is growing rapidly by extending current technologies, products and networks. By 2020, Cisco estimates there will be 50 billion connected devices. Gartner has forecast revenues of over $300 billion, just to IoT suppliers. Now is the time to figure out how you'll make money – not just create innovative products. With hundreds of new products and companies jumping into the IoT fray every month, there's no shortage of innovation. Despite this, McKinsey/VisionMobile data...
Oct. 8, 2015 05:30 PM EDT Reads: 138
As-a-service models offer huge opportunities, but also complicate security. It may seem that the easiest way to migrate to a new architectural model is to let others, experts in their field, do the work. This has given rise to many as-a-service models throughout the industry and across the entire technology stack, from software to infrastructure. While this has unlocked huge opportunities to accelerate the deployment of new capabilities or increase economic efficiencies within an organization, i...
Oct. 8, 2015 05:30 PM EDT Reads: 222
Containers are changing the security landscape for software development and deployment. As with any security solutions, security approaches that work for developers, operations personnel and security professionals is a requirement. In his session at @DevOpsSummit, Kevin Gilpin, CTO and Co-Founder of Conjur, will discuss various security considerations for container-based infrastructure and related DevOps workflows.
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Oct. 8, 2015 01:00 PM EDT Reads: 757


text:
Page 1
Thi s art i cl e w as dow nl oaded by: [59. 48. 106. 194]
On: 26 August 2015, At : 16:19
Publ i sher: Rout l edge
Inf orm a Lt d Regi st ered i n Engl and and W al es Regi st ered Num ber: 1072954 Regi st ered of f i ce: 5 How i ck Pl ace,
London, SW 1P 1W G
Nut ri t i on and Cancer
Publ i cat i on det ai l s, i ncl udi ng i nst r uct i ons f or aut hor s and subscr i pt i on i nf or m at i on:
ht t p: //www. t andf onl i ne. com /l oi /hnuc20
Gr ape Pol yphenol s I nhi bi t Akt / Mam m al i an Target of
Rapam yci n Si gnal i ng and Pot ent i at e t he Ef f ect s of
Gef i t i ni b i n Breast Cancer
Li net t e Cast i l l o- Pi char do a & Sur angani e F . Dhar m awar dhane a
a Depar t m ent of Bi ochem i st r y , School of Medi ci ne , Uni ver si t y of Puer t o Ri co , Medi cal
Sci ences Cam pus, San Juan , Puer t o Ri co
Publ i shed onl i ne: 12 Oct 2012.
To ci t e t hi s art i cl e: Li net t e Cast i l l o- Pi char do & Sur angani e F . Dhar m awar dhane ( 2012) Gr ape Pol yphenol s I nhi bi t Akt /
Mam m al i an Tar get of Rapam yci n Si gnal i ng and Pot ent i at e t he Ef f ect s of Gef i t i ni b i n Br east Cancer , Nut r i t i on and Cancer , 64: 7,
To l i nk t o t hi s art i cl e: ht t p: //dx. doi . or g/10. 1080/01635581. 2012. 716898
Tayl or & Franci s m akes every ef f ort t o ensure t he accuracy of al l t he i nf orm at i on (t he "Cont ent ") cont ai ned
i n t he publ i cat i ons on our pl at f orm . How ever , Tayl or & Franci s, our agent s, and our l i censors m ake no
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Cont ent . Any opi ni ons and vi ew s expressed i n t hi s publ i cat i on are t he opi ni ons and vi ew s of t he aut hors, and
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Page 2
Nutrition and Cancer, 64(7), 1058–1069
Copyright C ?2012, Taylor & Francis Group, LLC
ISSN: 0163-5581 print / 1532-7914 online
Grape Polyphenols Inhibit Akt/Mammalian Target
of Rapamycin Signaling and Potentiate the Effects
of Gefitinib in Breast Cancer
Linette Castillo-Pichardo and Suranganie F. Dharmawardhane
Department of Biochemistry, School of Medicine, University of Puerto Rico, Medical Sciences Campus,
San Juan, Puerto Rico
We recently reported that a combination of dietary grape
polyphenols resveratrol, quercetin, and catechin (RQC), at low
concentrations, was effective at inhibiting metastatic cancer pro-
inbreast cancer and explore thepotential of RQC as a potentiation
gefitinib. Our in vitro experiments showed RQC induced apoptosis
in gefitinib-resistant breast cancer cells via regulation of a myr-
iad of proapoptotic proteins. Because the Akt/mammalian target
of rapamycin (mTOR) signaling pathway is often elevated during
development of anti-EGFR therapy resistance, the effect of RQC
on the mTOR upstream effector Akt and the negative regulator
AMP kinase (AMPK) was investigated. RQC was found to reduce
Akt activity, induce the activation of AMPK, and inhibit mTOR
signaling in breast cancer cells. Combined RQC and gefitinib de-
creased gefitinib resistant breast cancer cell viability to a greater
extent than RQC or gefitinib alone. Moreover, RQC inhibited Akt
and mTOR and activated AMPK even in the presence of gefitinib.
Our in vivo experiments showed combined RQC and gefitinib was
more effective than the individual treatments at inhibiting mam-
mary tumor growth and metastasis in nude mice. Therefore, RQC
treatment inhibits breast cancer progression and may potentiate
anti-EGFR therapy by inhibition of Akt/mTOR signaling.
Breast cancer is the most commonly diagnosed form of can-
cer in women 40–55 yr of age and is the second cause of cancer
deaths (1). Recent clinical advances have increased cancer sur-
vival rates; however, de novo and acquired resistance to therapy
is common and contributes to disease relapse (2). Combina-
tion therapies that target several pathways are effective but can
have devastating side effects (3,4). Therefore, developing new
Submitted 26 February 2012; accepted in final form 23 July 2012.
Address correspondence to Suranganie F. Dharmawardhane, Ph.D.,
Department of Biochemistry, School of Medicine, University of Puerto
5067. Tel: 787-758-2525X1630. E-mail: email@example.com
strategies for prevention of breast cancer progression and over-
coming drug resistance represents a major challenge.
Grape polyphenols are desirable cancer therapeutics with
potential for combination therapy. Individual grape polyphe-
nols at high concentrations act as cancer preventives and have
antiproliferative, antioxidant, antiangiogenic, antiinvasive, and
pro-apoptotic properties (5). Moreover, grape polyphenols
specifically inhibit the growth of breast cancer cells with low
cytotoxicity toward normal mammary epithelial cells (6). These
compounds are of particular relevance for gynecological can-
cers such as breast cancer, because they have been shown to
act as selective estrogen receptor (ER) modulators and induce
differential gene expression via ERα and ERβ (7). Grape juice
constituents and grape seed extract (GSE) inhibit breast can-
cer initiation, reduce cancer in rodent models (8–10), and have
been associated with regulation of Akt and extracellular regu-
lated kinase (ERK) activities (11,12). However, the molecular
are not fully understood.
A growing debate on the cancer-preventive properties of
natural compounds is that dietary consumption is insufficient
to achieve cancer inhibitory concentrations at target tissues
(13–17). However, resveratrol, quercetin, or catechin (RQC)
are all considered viable chemopreventives because they are
absorbed and metabolized rapidly in vivo and can be detected
in plasma and urine samples in the intact form in humans and
rodent models (13–18).
RQC are usually conjugated to glucoronic acid and sulfate
during first-pass metabolism in the intestinal wall or the liver,
although a portion of these metabolites can also be methy-
lated (19–21). Glucorono-conjugates are hydrolyzed, yielding
the aglycone, which is more lipophilic and can remain trapped
in the tissues (19,22). Therefore, although the aglycone forms
of RQCs are rapidly cleared from plasma, they can still be
found in considerable amounts in tissues, together with conju-
gated metabolites (22). Accordingly, following oral consump-
tion, these polyphenols are found mainly in the serum and urine
as glucuronide and sulfate conjugates. In a human study, free
Downloaded by [184.108.40.206] at 16:19 26 August 2015
Page 3
polyphenols in the serum accounted for 1.7%–1.9% (resver-
atrol), 1.1%–6.5% (catechin), and 17.2%–26.9% (quercetin),
although more than 80% was absorbed (23). Therefore, stud-
ies that quantified plasma levels of polyphenols may be un-
derestimating the actual amounts that reach the tissues, and
more specifically the site of action. Although, more epidemi-
ological data on the effects of grape polyphenols in humans
need to be collected, a study reported that grape consumption
was significantly inversely associated with breast cancer risk
(24). However, few studies have documented the effect of grape
polyphenols on metastatic breast cancer.
cer progression and metastasis, including breast cancer, where
ical trials have demonstrated the utility of gefitinib, an EGFR-
specific tyrosine kinase inhibitor (26). However, de novo and
acquired resistance to gefitinib treatment is a recurrent problem
(27,28). Some of the early trials on the efficacy of gefitinib in
breast cancer were negative or discontinued because of adverse
effects or lack of response probably via resistance mechanisms
(29–32). Therefore, multiple studies have investigated the po-
(33). A number of recent trials have reported the efficacy of
gefitinib in combination with other therapeutics for both human
epidermal growth factor receptor 2 (HER2) positive and ER
positive breast cancers (34–38). Grape polyphenols are attrac-
tive candidates for potentiation to EGFR therapy because they
have been shown to individually inhibit EGFR 1 and 2 (HER2)
activities (39,40), and decrease HER2 expression, and regulate
pathways that confer therapy resistance (41).
for malignancy and evasion to gefitinib treatment (42,43). Akt
activates mTOR complex 1 (mTORC1), which contains mTOR
and regulatory associated protein of mTOR (Raptor), via inhi-
bition of tuberous sclerosis protein (TSC) 2. TSC2 is a GTPase
activating protein (GAP) and thus, a negative regulator of Rheb
GTPase that activates mTOR (44). AMP kinase (AMPK), a key
to inhibit mTORC1 via an activating phosphorylation of TSC2
and by direct inhibition of Raptor (45). Therefore, dual PI3-
K/mTOR inhibitors have potential in aggressive cancer therapy
(46). Because combination therapy can have devastating side
effects, a safer alternative is the use of dietary compounds, such
as grape polyphenols, with low toxicity that can inhibit both
pathways (47–51).
We recently reported that a combination of the major
polyphenols in grape and red wine, RQC, at low concentrations,
can inhibit proliferation, cell cycle progression, migration, and
survival in breast cancer cells and can reduce tumor growth
and site-specific metastasis (52,53). Herein, we show that RQC
induces apoptosis and reduces mTOR signaling probably via
inhibition of Akt and activation of AMPK in breast cancer and,
in combination with gefitinib, can reduce cell proliferation, tu-
mor growth, and metastasis of a gefitinib resistant breast cancer
cell line.
Cell Culture
The human metastatic breast cancer cell line MDA-MB-231
[ERα(-), ERβ(+)] expressing green fluorescent protein (GFP)
(kind gift of Dr. Danny Welch, The University of Alabama at
Birmingham, AL, in 2009) (54) was cultured, as described in
Ref. 52.
RQC were purchased as 99% pure compounds (LKT Lab-
oratories, St. Paul, MN) and stock solutions made in dimethyl
sulfoxide (DMSO) or ethanol. Stock solutions for gefitinib (LC
Laboratories, Woburn, MA) were prepared in DMSO.
Caspase 3 Activity Assay
of MDA-MB-231 cell lysates following vehicle (0.2% DMSO)
or RQC at 5 µM each for 48 h or 96 h using a Caspase-3 Colori-
metric Assay Kit as per manufacturer's instructions (Sigma-
Aldrich, St. Louis, MO). Briefly, the p-nitroaniline moiety
resulting from caspase 3-mediated hydrolysis of acetyl-Asp-
Glu-Val-Asp p-nitroaniline was detected at 405 nm (εmM =
10.5) after incubating the reaction mixture at 37◦C for 22 h.
Antibody Arrays
Total protein extracts from MDA-MB-231 cells treated for
48 h or 96 h with vehicle, or 5 µM RQC were incubated
overnight with a human apoptosis antibody array (R&D Sys-
tems, Minneapolis, MN), as per manufacturer's instructions.
Unbound proteins were washed away and the array incubated
with a cocktail of biotinylated antibodies that were detected
using streptavidin-Horseradish peroxidase chemiluminescent
Data was analyzed using average integrated density signal
(calculated using Image J software) for the 2 replicates of each
antibody. Then, the 2 sets of data were normalized using a
controls in 1 data set vs. the other. Fold changes compared to
vehicle controls were calculated from averages of 3 biological
Western Blotting
Quiescent MDA-MB-231 cells were treated with vehicle, or
5 µM RQC at 5 µM each; or 5 µM RQC, 30 µM gefitinib,
or a combination of 5 µM RQC and 30 µM gefitinib, for 15 or
30 min. Cells were immediately lysed as in Ref. 53, and total
protein was quantified using the Precision Red protein assay kit
(Cytoskeleton, Inc., Denver, CO). Equal total protein amounts
were Western blotted using anti-Akt, antiphospho AktS473, anti-
AMPKα, antiphospho AMPKαThr172, anti-ribosomal p70 S6 ki-
nase (p70S6K), or antiphospho p70S6KThr389(Cell Signaling
Downloaded by [188.8.131.52] at 16:19 26 August 2015
Page 4
Technology, Inc.,Danvers,MA)antibodies.Theintegratedden-
sity of positive bands was quantified using Image J software.
Cell Viability
MDA-MB-231 (5 × 104) cells in 6 well plates were treated
for 48 h (in culture media with 5% fetal bovine serum) with
vehicle, or RQC (0.167, 0.5, 1.67, 5, or 15 µM), gefitinib (0.1,
1, 10, 20, 30, or 40 µM), or RQC and gefitinib (0.5, 1.67, or
5 µM RQC and 10, 20, or 30 µM gefitinib). Cells were fixed,
nuclei stained with propidium iodide (PI), and the viable cells
For the generation of dose-response curves and the determi-
nation of IC50values, data from 3 independent experiments was
pooled and 4 parameter dose-response curves were fitted using
the nonlinear regression function of GraphPad PrismR ?.
Hairless severe combined immunodeficiency (SCID) female
mice, 5 to 6 wk old (Charles River Laboratories, Inc., Wilm-
ington, MA) were maintained under pathogen-free conditions
in Hepa-filtered cages (5 mice per cage) under controlled light
(12-h light and dark cycle), temperature (22◦C to 24◦C), and
humidity (25%). The animals received autoclaved AIN 76-A
phytoestrogen-free diet (Tek Global, Harlan Teklad, Madison,
WI) with 14% protein and 3.5% fat and water ad libitum. All
the animal procedures were conducted in a room dedicated to
immunocompromised mice at the institution's animal facility.
Anesthetized animals were euthanized by cervical dislocation.
This study was carried out in accordance with the recommenda-
tions in the Guide for the Care and Use of Laboratory Animals
of the National Institutes of Health.
Tumor Establishment
GFP-MDA-MB-231 cells (∼1 × 106) in Matrigel (BD Bio-
sciences, San Jose, CA) were injected at the fourth right mam-
mary fat pad under isofluorane inhalation (1%–3% in air using
inhalation chamber or nose cone, at 2 L/min) to produce ortho-
topic primary tumors as described in Ref. 53. Isofluorane was
chosen as the anesthetic agent as it requires minimal animal
handling and has a large margin of safety and quick recovery
times. After tumor establishment (1 wk postinoculation), the
animals from the same litter with similar weight and tumor
size were randomly divided into experimental treatment groups
(n = 10–12 per treatment group).
Diet Administration
Mice were orally gavaged either with vehicle for RQC treat-
ment (90% neobee oil, 10% ethanol), vehicle for gefitinib (1%
Tween-80 in 1× PBS, pH 7.4), a combination of 5 mg/kg body
weight (BW) resveratrol, 5 mg/kg BW quercetin, and 5 mg/kg
BW catechin (RQC), or 200 mg/kg BW gefitinib, in a 100 µL
volume every other day, 7 days a week. The group receiving
both treatments was gavaged 1 day with 5 mg/kg BW RQC, and
the next day with 200 mg/kg BW gefitinib in a 100 µL volume,
7 days a week. Treatments continued until sacrifice at day 84.
Whole Body Fluorescence Image Analysis
Mammary tumor growth was quantified as changes in the
integrated density of GFP fluorescence, using methods devel-
oped by Hoffman and coworkers (55). Mice were imaged 1
wk following breast cancer cell inoculation (on day 1 of diet
administration) and once a week thereafter. A 300 watt power
source with 2 optical delivery systems fitted with excitation fil-
ters (470/40 nm) was used for whole body imaging of GFP
fluorescence (LT99D2, Lightools Research, Encinitas, CA).
Images were captured with a Spot II charge-coupled device
(CCD) camera (Diagnostic Instruments, Sterling Heights, MI)
Rockingham, VT).
Tumor fluorescence intensities were analyzed using Image
J software (National Institutes of Health, Bethesda, MD). The
final images were acquired on day 84. Relative tumor growth
was calculated as the integrated density of fluorescence of each
fluorescence of the same tumor on day 1 of diet administration,
as described in Refs. 52 and 53. Tumor growth in vehicle for
RQC group was not statistically different than tumor growth
in vehicle for gefitinib group. Therefore, the average of both
groups was used for data analysis.
Analysis of Metastases
Following sacrifice, lungs, kidneys, and livers were excised
and analyzed using an Olympus MV10 fluorescence macro
zoom system microscope and images acquired with an Olym-
pus DP71 digital camera, as described in Refs. 52 and 53. Each
organ was imaged on both sides. The fluorescent lesions (green
component of RGB images) were quantified for integrated den-
sity of fluorescent pixels using Image J software.
Statistical Analysis
Data are expressed as the mean ± SEM. Statistical analyses
were done using Microsoft Excel and GraphPad PrismR ?. Dif-
ferences between means were determined using Student's t-test
or 1-way analysis of variance with Dunnett's Multiple Compar-
ison Test. The Kruskal-Wallis test with Dunn's Multiple Com-
parison was used for groups that did meet the requirements of
normality and/or equal variance for 1-way analysis of variance.
Differences between groups are considered to be statistically
significant at P ≤ 0.05.
Effects of Combined Grape Polyphenols on Apoptosis and
Apoptotic Signaling Proteins
We previously reported that the RQC combination of grape
polyphenols was more effective than RQC at inhibiting cancer
ERα(-), ERβ(+) MDA-MB-231 low metastatic breast cancer
cells and ER(-) MDA-MB-435 highly metastatic cancer cells
Downloaded by [126.96.36.199] at 16:19 26 August 2015
Page 5
(52,53). In addition, RQC induced apoptosis of MDA-MB-435
cells (53). Because RQC treatment at 5 µM each, for 96 h,
decreased MDA-MB-231 cell viability by ∼80% compared to
vehicle controls and caused S phase cell cycle arrest (53), the
effect of 5 µM RQC treatment on apoptosis of MDA-MB-231
cells was determined via caspase 3 activity. RQC at 5 µM did
not induce apoptosis in 48 h, whereas treatment with 5 µM
RQC for 96 h increased caspase 3 activity by 3-fold with a P
≤ 0.05 thus, implicating RQC treatment in apoptosis regulation
(Fig. 1A).
The molecular mechanisms of RQC-induced apoptosis were
studied using human apoptotic antibody arrays. Treatment of
MDA-MB-231 cells with combined grape polyphenols at 5 µM
in the apoptotic signaling cascade (Fig. 1B, Table 1). Table 1
summarizes results from western arrays of apoptotic signaling
proteins that were significantly regulated by RQC treatment for
48 h and 96 h in 3 independent experiments. At 48 h, prosur-
vival proteins such as cellular inhibitor of apoptosis protein 1
3 activity, indicating that regulation of expression of apoptosis
inhibitors precedes induction of apoptosis by RQC. At 96 h, a
significant increase in cleaved caspase 3 and FAS ligand and its
receptor was observed, correlative to RQC-increased caspase
3 activity (Fig. 1, Table 1). These results indicate a potential
death receptor-mediated regulation of apoptosis by RQC. The
observed decrease in Hemeoxygenase 1 (HO-1) levels by RQC
is in contrast to reports of quercetin upregulating HO-1 in re-
sponse to oxidant stress in normal cells (56). However, because
our study was conducted with aggressive cancer cells in an ox-
idant stress-free environment, this result may reflect a cancer
cell-specific effect or a combination effect of RQC.
Effects of RQC on Regulation of the mTOR Pathway
To further investigate the molecular mechanisms by which
RQC reduces breast cancer cell survival, we determined the
effect of RQC on PI3-K/Akt signaling by determining the ac-
tivation status, i.e. phosphorylation levels, of Akt. Akt is phos-
phorylated and activated by phosphoinositide-dependent kinase
when phosphatidylinositol 3,4,5-trisphosphate (PIP3) levels are
increased because of PI3-K activity. In MDA-MB-231 cells,
5 µM treatment with individual quercetin or RQC for 15 min
resulted in a ∼50% inhibition of Akt activity without affecting
total Akt expression (Fig. 2A). Treatment with 5 µM RQC for
15 min also decreased Akt activity significantly in MDA-MB-
435 cells (data not shown).
We previously reported that combined RQC was more effi-
cient than individual compounds at inhibiting breast cancer cell
is expected to inhibit breast cancer progression via additional
molecular mechanisms. The activities of AMPK, a negative
regulator of mTOR, and mTOR were determined by Western
blot analysis of total and phosphorylated AMPK and p70S6K,
3 activity assays following 48 and 96 h incubation with vehicle (Veh) or combined resveratrol (Res), quercetin (Quer), and catechin (Cat) at 5 µM each (RQC).
A: Caspase 3 activity relative to Veh (n = 3 ± SEM) as quantified from absorbance at 405 nm of p-nitroaniline, a product released by caspase 3 activity. An
asterisk indicates statistical significance (P ≤ 0.05) when compared to vehicle. B: Apoptotic signaling proteins regulated in MDA-MB-231 cells at 48 h following
Veh or 5 µM RQC treatment were studied using antibody arrays. Normalized data was analyzed using average integrated density signal for the two replicates of
each antibody. A representative image of 3 biological replicates is presented. Proteins that were significantly regulated by RQC are highlighted.
Effect of resveratrol, quercetin, and catechin (RQC) on apoptosis of MDA-MB-231 cells. Apoptosis of MDA-MB-231 cells was detected by caspase
Downloaded by [18.104.22.168] at 16:19 26 August 2015


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India has called on Saudi Arabia to cooperate in rape investigation
Indian police registered an allegation of rape, sodomy and illegal confinement against the unnamed official
Welcome to the new Independent website. We hope you enjoy it and we value your feedback. Please contact us here.
Indian police registered an allegation of rape, sodomy and illegal confinement against the unnamed official
Surgery which was donated after viral pictures, but the baby will still require lots of medical care
The condition of a 5-year-old girl who was allegedly kidnapped, raped and tortured by a man and then left alone in a locked room in India's capital for two days has improved, a doctor said today, as protests continued over the authorities' handling of the case.
Force of 7.8 magnitude quake could be felt from Kabul to Delhi and Dubai
Hardliners defied as historic blueprint to tackle violence against girls and women is agreed at the United Nations
Andrew Buncombe sees the five men accused of student's murder face a barrage of abuse as they arrive for Delhi hearing
Officials carried out a bone test today to confirm the age of a young suspect in custody over the fatal assault and gang rape of a woman on a bus in India's capital, while prosecutors will seek the death penalty for five men arrested with him.
A young woman who died after being gang-raped and beaten on a bus in India was cremated today amid an outpouring of anger and grief across the country by millions of people demanding greater protection for women from sexual violence.
The Indian government sought to douse protests against rape by simultaneously clamping down and announcing a slew of measures to assuage demonstrators, including a new help line for women's complaints and gender-appropriate training for the capital's police officers.
Laptop bag in one hand and BlackBerry in the other, investment banker Saurabh Chawla slid into his black Mercedes-Benz one morning and headed to his job in the Indian capital.
Indian authorities investigating a rape case say a sprinter who won a women's relay gold medal at the 2006 Asian Games is actually a man.
Formula One championship rivals Sebastian Vettel of Red Bull and Ferrari's Fernando Alonso both have cause for optimism about their victory chances in this weekend's Indian Grand Prix, as the title fight gets down to its final four races.
So who is this? He seems important…
Hamilton hails new track but suffers grid penalty after setting fastest time in first practice session
Two wild elephants have killed a man and injured others on a rampage in southern India.
Police fired tear gas yesterday to break up a hunger strike by a charismatic Indian yoga guru who is calling for an end to endemic state corruption. Officers forcibly removed him and thousands of his followers from a tent in New Delhi.
Lancelot Ribeiro was one of the most original Indian painters who settled in Britain after the Second World War. Although there has been a surge of dealer and collector interest in artists from the subcontinent, Ribeiro remains relatively unknown compared with contemporaries such as his half-brother FN Souza, Avinash Chandra, Balraj Khanna and Anish Kapoor.


text:
Education Bills a Breath of Fresh Air for Pesticide Foes
Mask, Teresa, Daily Herald (Arlington Heights, IL)
Byline: Teresa Mask Daily Herald Staff Writer
Stopping the use of pesticide sprays in schools is a battle Hinsdale resident Diana Fleming has been fighting for 13 years.
She said her son, Doug, now in college, had recurring asthma attacks while a student in Hinsdale Elementary District 181 as a result of exposure to pesticides.
Experts say other chemical reactions include rashes and respiratory problems. Some exposure has been known to lead to neurological problems and cancer.
Illinois legislators and health experts have encouraged school officials for years to adopt alternate pest-control measures to protect students sensitive to chemicals.
This week it appears legislators are moving a step closer to forcing school districts to use pesticide sprays only as a last resort.
The Senate education committee approved two bills to that end. One would require 48-hour notice to parents before any pesticides are used on school grounds, allowing those with chemical sensitivity to take precautionary measures. The other would require schools to use alternative pest-control measures.
"It's time for Illinois to join states across the country that are requiring IPM (integrated pest management)," said state Sen. …


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Dexter is back! The critically acclaimed and groundbreaking original series from Showtime starring Michael C. Hall, returns to DVD with all 12 riveting and chilling episodes in Dexter: The Second Season. Although committed to carrying out his twisted brand of vigilante justice-Dexter begins to doubt his murderous capabilities and continues to be haunted by his tortured past. His difficulties multiply when evidence of his deadly after-hours activities begin to surface and the FBI is brought in to investigate the city's new serial killer dubbed the 'Bay Harbor Butcher.' Dexter cant pause for a breathe as the noose tightens and the questions keep mounting. Will he be able to continue his serial-killing ways? Or will Dexter's dark past finally be uncovered?
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addicted to this series. you will fall in love with all the characters.Published 1 day ago by Jennifer Gerwick
First couple seasons of this show are great, 1-4. The later seasons are sortof weird, but I like the first few! DVDs play fine.Published 1 month ago by Charlie Forrester
Dexter vs Sgt. Doakes and the sexy "torch artist" and many more. Get it and enjoy.Published 1 month ago by Poet D
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|Warning! Bootleg Dexter DVDs on the internet
This is detailing my exact senerio...couldn't believe the Show Time Coupons, that was classic! A couple of the discs were acting up so I contacted the seller on eBay for a refund. The seller told me I could keep the DVDs and he would issue me a full refund within 3 days. Paid $60 for seasons... Read More
Feb 5, 2012 by Matthew R. Christie | See all 3 posts
|Dexter Season 2 Release Date, please
"SEE?? This is the crap I'm talking about! People posting stuff before it's available is VERY misleading and unfair to people interested in buying what's being sold!"
I think you missed the point of the post. What's being discussed is obviously not a review of the DVD as it hasn't been... Read More
May 8, 2008 by Stephen Hearn | See all 16 posts
|Dexter Season 2 Cheaper at Target
...and all these months later (it's June 7th), I just got it at Target for $18.99! Target just rocks ... period!
Jun 7, 2009 by R. J. Werner | See all 3 posts
|The Guardian
This show is awesome. Season Two is playing "On Demand" if you subscribe to Showtime and Season Three is set to begin sometime in September - next month! So check your local listings. It really seemed like forever between seasons so I know how you must have thought the show disappeared.... Read More
Aug 7, 2008 by G. Faretra-Reilly | See all 3 posts
I phone Amazon, they told me they received last Wenesday an email from the distributor saying that the release has been delayed and that they did not know when it was comming out, so they have cancelled all orders since now there is no specific date of release. Same thing happen in all websites,... Read More
Apr 19, 2008 by Hermes Navarro | See all 5 posts
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Back in July, the city of Chicago started levying an unpopular new tax on its residents: cloud services, including streaming media like Netflix, suddenly cost 9% extra. Neither denizens of the Windy City nor the businesses who serve them are at all happy with this, however, and now the lawsuits have begun. [More]
Chicago Sued Over 9% "Cloud Tax" On Streaming Services
New Spotify Privacy Policy Aims To Provide "Plain Language" Explanation For Collected Data
Just weeks after Spotify ticked off many of its 75 million active users with an updated, potentially invasive privacy policy, the music streaming service has once again revamped the terms, this time including an introduction that provides clear reasoning and examples of data collected. [More]
What Does Spotify's New Privacy Policy Actually Say, And Should I Be Worried?
Spotify has basically run away with the music market over the last couple of years, boasting over 75 million active users. But the popular streaming service this week ticked off a bunch of those customers this week when it updated its privacy policy and user terms and conditions. And their timing couldn't have been worse: the combination of seeming to add a dramatic and invasive new set of permissions to their apps, in a week when privacy concerns and hacks are already the top headline, set off an angry internet firestorm. [More]
Sen. Al Franken Calls For Federal Investigation Into Apple Music
In response to concerns arising from the recent launch of Apple Music, U.S. Senator Al Franken called on both the Department of Justice and the Federal Trade Commission to investigate the possibility that the tech giant may be creating an anticompetitive environment in the streaming music market. [More]
Starbucks Teams Up With Lyft To Give Drivers, Riders Extra Points Through Its Rewards Program
Take a Lyft, earn a free cup of coffee? Well, something like that anyway. Starbucks and ride-sharing service Lyft teamed up on Wednesday to unveil a new arrangement that gives customers – and drivers – of the car service extra perks through the coffee chain's loyalty program. [More]
Spotify Wants iOS Users To Skip Apple's App Store, Save $3
Apple's new streaming music service costs $10 per month. So does competing service Spotify, but if you subscribe through the Spotify iPhone app, they charge you an extra three bucks because of Apple's 30% cut of every subscription sold through apps on their devices. However, Apple's rules for what's okay to put in an app mean that Spotify can't actually tell you this, so they sent an e-mail to users explaining how to change your subscription. [More]
App Loops 30-Second Spotify Snippets To Support Your Favorite Artist
Do you want to support your favorite musicians, but do so without spending any money and without actually listening to their songs? A band came up with a brilliant scheme to extract as much royalty cash from Spotify for your favorite musicians in the shortest amount of time. It's called Eternify, and it renders your favorite songs unlistenable. [More]
Apple Music Offers Musicians Royalties Of 70% Of Nothing During Free Trial
Apple's streaming music service is coming to a device near you at the end of this month, since it's likely that there's some kind of device with iTunes on it near you right now. Yet while Apple is promising musicians over 70% of the revenue from the service as royalties, that also means musicians will get around 70% of nothing for the first three months of Apple Music, since the service will be free to users. [More]
Pandora CFO Not Worried By Apple Music Announcement
Yesterday's Apple Music announcement was seen as a long-overdue swipe at streaming music services like Pandora and Spotify that have dominated the market that Apple helped to create with the introduction of the iPod and iTunes, along with the iPhone and iPad mobile devices that people listen to the music on. Given Apple's ability to instantly reach the large swath of iOS users, you wouldn't fault these competitors from being frightened, but the Chief Financial Officer of Pandora doesn't seem terribly worried. [More]
Apple Knows You Hate iTunes And Love Spotify, So They're Launching A Streaming Music Service Too
Apple's iTunes digital storefront for music was a pretty big deal when it launched over a decade ago. But time, and data plans, march on. Where once being able to buy a cheap single was the new hotness, these days consumers are more likely to want to stream their music, through a service like Spotify or Pandora. And so, Apple being Apple, they're about to launch a new streaming service too. [More]
Starbucks & Spotify Team Up In Streaming Music Deal
Just two months after Starbucks stopped selling CDs in its stores, the coffee chain says it's going to be filling customers' ears with music another way, announcing a streaming partnership with Spotify that will let customers have a say in what's being played. [More]
Warner Music Is Totally Cool With Streaming Music Now That It's Making More Money Than Song Downloads
Once seen as the knife that would slay the music industry, it seems the popularity — and profitability — of streaming music is making some labels change their tune. Warner Music Group said revenue from streaming music has passed that from digital downloads for the first time. [More]
Report: DOJ Has An Eye On Apple As It Makes Moves To Push Free Streaming Music Rivals Out Of The Way
Let's be honest: It would be ideal for a business if it could somehow snap up every customer looking for a certain service. But because we (thankfully) live in a world filled with choices, companies instead must compete with rivals to get our hard-earned dollars. A new report says Apple is preparing to debut its new streaming music service by trying to put the squeeze on competitors like Spotify that offer content for free. [More]
Sony Ditches Its Streaming Music Service, Teams With Spotify For Playstation Music
If at first you don't succeed, try again using someone else's system: Sony is pulling the plug on its current streaming music offering, Music Unlimited, and hitching its wagon to Spotify's star with a new partnership for PlayStation. [More]
Uber And Spotify Pair Up To Allow Riders To Choose Their Own Tunes For The Drive
"What do you mean when you say you 'have no Janet Jackson'??!?!" at least one car full of people has gaspscreamed at at least one car service driver (sorry, guy). That outraged reaction could be a thing of the past for Uber customers, as the company has joined forces with Spotify to offer Spotify Premium account holders the ability to play their own playlists and control the music during some Uber rides. [More]
Spotify Launches Family Plan That Allows Users To Share A Discounted Subscription
As competition heats up in the world of streaming music, Spotify is following the lead of other streaming services like Netflix with a new family plan. The option allows up to five family members to subscribe together for a monthly discounted rate. [More]
Spotify Hacked; Asking Android Users To Upgrade
Welcome back from the holiday. What better way to celebrate that morning-after feeling with news that the popular music streaming service Spotify has been hacked and now you have to change your password and/or upgrade your app? [More]
Spotify Has 10 Million Paying Customers, Still Loses Money
There have been three broad eras so far of online music. There was the era of Napster, when rampant piracy was great for music-loving young people, and a seeming catastrophe for artists and record labels. There was the era of iTunes, which allowed fans to buy only the single song that they wanted to hear. Now the era of Spotify lets us simply stream music on a variety of devices. It's convenient, but is it sustainable for everyone…especially Spotify, which still doesn't turn a profit? [More]


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Here is a collection of the all-time best famous Robert Herrick poems. This is a select list of the best famous Robert Herrick poetry. Reading, writing, and enjoying famous Robert Herrick poetry (as well as classical and contemporary poems) is a great past time. These top poems are the best examples of Robert Herrick poems.
Search for the best famous Robert Herrick poems, articles about Robert Herrick poems, poetry blogs, or anything else Robert Herrick poem related using the PoetrySoup search engine at the top of the page.
Weigh me the fire; or canst thou find
A way to measure out the wind?
Distinguish all those floods that are
Mixed in that wat'ry theater,
And taste thou them as saltless there,
As in their channel first they were.
Tell me the people that do keep
Within the kingdoms of the deep;
Or fetch me back that cloud again,
Beshivered into seeds of rain.
Tell me the motes, dust, sands, and spears
Of corn, when summer shakes his ears;
Show me that world of stars, and whence
They noiseless spill their influence.
This if thou canst; then show me Him
That rides the glorious cherubim.
In man, ambition is the common'st thing;
Each one by nature loves to be a king.
Sweet country life, to such unknown,
Whose lives are others', not their own!
But serving courts and cities, be
Less happy, less enjoying thee.
Thou never plough'st the ocean's foam
To seek and bring rough pepper home:
Nor to the Eastern Ind dost rove
To bring from thence the scorched clove:
Nor, with the loss of thy loved rest,
Bring'st home the ingot from the West.
No, thy ambition's master-piece
Flies no thought higher than a fleece:
Or how to pay thy hinds, and clear
All scores: and so to end the year:
But walk'st about thine own dear bounds,
Not envying others' larger grounds:
For well thou know'st, 'tis not th' extent
Of land makes life, but sweet content.
When now the cock (the ploughman's horn)
Calls forth the lily-wristed morn;
Then to thy corn-fields thou dost go,
Which though well soil'd, yet thou dost know
That the best compost for the lands
Is the wise master's feet, and hands.
There at the plough thou find'st thy team,
With a hind whistling there to them:
And cheer'st them up, by singing how
The kingdom's portion is the plough.
This done, then to th' enamell'd meads
Thou go'st; and as thy foot there treads,
Thou seest a present God-like power
Imprinted in each herb and flower:
And smell'st the breath of great-eyed kine,
Sweet as the blossoms of the vine.
Here thou behold'st thy large sleek neat
Unto the dew-laps up in meat:
And, as thou look'st, the wanton steer,
The heifer, cow, and ox draw near,
To make a pleasing pastime there.
These seen, thou go'st to view thy flocks
Of sheep, safe from the wolf and fox,
And find'st their bellies there as full
Of short sweet grass, as backs with wool:
And leav'st them, as they feed and fill,
A shepherd piping on a hill.
For sports, for pageantry, and plays,
Thou hast thy eves, and holydays:
On which the young men and maids meet,
To exercise their dancing feet:
Tripping the comely country Round,
With daffadils and daisies crown'd.
Thy wakes, thy quintels, here thou hast,
Thy May-poles too with garlands graced;
Thy Morris-dance; thy Whitsun-ale;
Thy shearing-feast, which never fail.
Thy harvest home; thy wassail bowl,
That's toss'd up after Fox i' th' hole:
Thy mummeries; thy Twelve-tide kings
And queens; thy Christmas revellings:
Thy nut-brown mirth, thy russet wit,
And no man pays too dear for it.--
To these, thou hast thy times to go
And trace the hare i' th' treacherous snow:
Thy witty wiles to draw, and get
The lark into the trammel net:
Thou hast thy cockrood, and thy glade
To take the precious pheasant made:
Thy lime-twigs, snares, and pit-falls then
To catch the pilfering birds, not men.
--O happy life! if that their good
The husbandmen but understood!
Who all the day themselves do please,
And younglings, with such sports as these:
And lying down, have nought t' affright
Sweet Sleep, that makes more short the night.
More great poems below...
BE those few hours, which I have yet to spend,
Blest with the meditation of my end;
Though they be few in number, I'm content;
If otherwise, I stand indifferent,
Nor makes it matter, Nestor's years to tell,
If man lives long, and if he live not well.
A multitude of days still heaped on
Seldom brings order, but confusion.
Might I make choice, long life should be with-stood;
Nor would I care how short it were, if good;
Which to effect, let ev'ry passing bell
Possess my thoughts, next comes my doleful knell;
And when the night persuades me to my bed,
I'll think I'm going to be buried;
So shall the blankets which come over me
Present those turfs, which once must cover me;
And with as firm behaviour I will meet
The sheet I sleep in, as my winding-sheet.
When Sleep shall bathe his body in mine eyes,
I will believe, that then my body dies;
And if I chance to wake, and rise thereon,
I'll have in mind my resurrection,
Which must produce me to that Gen'ral Doom,
To which the peasant, so the prince must come,
To hear the Judge give sentence on the Throne,
Without the least hope of affection.
Tears, at that day, shall make but weak defense,
When Hell and horror fright the conscience.
Let me, though late, yet at the last, begin
To shun the least temptation to a sin;
Though to be tempted be no sin, until
Man to th'alluring object gives his will.
Such let my life assure me, when my breath
Goes thieving from me, I am safe in death;
Which is the height of comfort, when I fall,
I rise triumphant in my funeral.
Here, a little child, I stand,
Heaving up my either hand:
Cold as paddocks though they be,
Here I lift them up to thee,
For a benison to fall
On our meat, and on us all.
Come pity us, all ye who see
Our harps hung on the willow-tree;
Come pity us, ye passers-by,
Who see or hear poor widows' cry;
Come pity us, and bring your ears
And eyes to pity widows' tears.
CHOR. And when you are come hither,
Then we will keep
A fast, and weep
Our eyes out all together,
For Tabitha; who dead lies here,
Clean wash'd, and laid out for the bier.
O modest matrons, weep and wail!
For now the corn and wine must fail;
The basket and the bin of bread,
Wherewith so many souls were fed,
CHOR. Stand empty here for ever;
And ah! the poor,
At thy worn door,
Shall be relieved never.
Woe worth the time, woe worth the day,
That reft us of thee, Tabitha!
For we have lost, with thee, the meal,
The bits, the morsels, and the deal
Of gentle paste and yielding dough,
That thou on widows did bestow.
CHOR. All's gone, and death hath taken
Away from us
Our maundy; thus
Thy widows stand forsaken.
Ah, Dorcas, Dorcas! now adieu
We bid the cruise and pannier too;
Ay, and the flesh, for and the fish,
Doled to us in that lordly dish.
We take our leaves now of the loom
From whence the housewives' cloth did come;
CHOR. The web affords now nothing;
Thou being dead,
The worsted thread
Is cut, that made us clothing.
Farewell the flax and reaming wool,
With which thy house was plentiful;
Farewell the coats, the garments, and
The sheets, the rugs, made by thy hand;
Farewell thy fire and thy light,
That ne'er went out by day or night:--
CHOR. No, or thy zeal so speedy,
That found a way,
By peep of day,
To feed and clothe the needy.
But ah, alas! the almond-bough
And olive-branch is wither'd now;
The wine-press now is ta'en from us,
The saffron and the calamus;
The spice and spikenard hence is gone,
The storax and the cinnamon;
CHOR. The carol of our gladness
Has taken wing;
And our late spring
Of mirth is turn'd to sadness.
How wise wast thou in all thy ways!
How worthy of respect and praise!
How matron-like didst thou go drest!
How soberly above the rest
Of those that prank it with their plumes,
And jet it with their choice perfumes!
CHOR. Thy vestures were not flowing;
Nor did the street
Accuse thy feet
Of mincing in their going.
And though thou here liest dead, we see
A deal of beauty yet in thee.
How sweetly shews thy smiling face,
Thy lips with all diffused grace!
Thy hands, though cold, yet spotless, white,
And comely as the chrysolite.
CHOR. Thy belly like a hill is,
Or as a neat
Clean heap of wheat,
All set about with lilies.
Sleep with thy beauties here, while we
Will shew these garments made by thee;
These were the coats; in these are read
The monuments of Dorcas dead:
These were thy acts, and thou shalt have
These hung as honours o'er thy grave:--
CHOR. And after us, distressed,
Should fame be dumb,
Thy very tomb
Would cry out, Thou art blessed.
Come, Anthea, let us two
Go to feast, as others do:
Tarts and custards, creams and cakes,
Are the junkets still at wakes;
Unto which the tribes resort,
Where the business is the sport:
Morris-dancers thou shalt see,
Marian, too, in pageantry;
And a mimic to devise
Many grinning properties.
Players there will be, and those
Base in action as in clothes;
Yet with strutting they will please
The incurious villages.
Near the dying of the day
There will be a cudgel-play,
Where a coxcomb will be broke,
Ere a good word can be spoke:
But the anger ends all here,
Drench'd in ale, or drown'd in beer.
--Happy rusticks! best content
With the cheapest merriment;
And possess no other fear,
Than to want the Wake next year.
In prayer the lips ne'er act the winning part
Without the sweet concurrence of the heart.
Honour to you who sit
Near to the well of wit,
And drink your fill of it!
Glory and worship be
To you, sweet Maids, thrice three,
Who still inspire me;
And teach me how to sing
Unto the lyric string,
My measures ravishing!
Then, while I sing your praise,
My priest-hood crown with bays
Green to the end of days!
Anthea, I am going hence
With some small stock of innocence;
But yet those blessed gates I see
Withstanding entrance unto me;
To pray for me do thou begin;--
The porter then will let me in.
Love is a circle, that doth restless move
In the same sweet eternity of Love.
When I a verse shall make,
Know I have pray'd thee,
For old religion's sake,
Saint Ben to aid me.
Make the way smooth for me,
When I, thy Herrick,
Honouring thee, on my knee
Offer my lyric.
Candles I'll give to thee,
And a new altar,
And thou, Saint Ben, shalt be
Writ in my psalter.
Thou art to all lost love the best,
The only true plant found,
Wherewith young men and maids distrest
And left of love, are crown'd.
When once the lover's rose is dead
Or laid aside forlorn,
Then willow-garlands, 'bout the head,
Bedew'd with tears, are worn.
When with neglect, the lover's bane,
Poor maids rewarded be,
For their love lost their only gain
Is but a wreath from thee.
And underneath thy cooling shade,
When weary of the light,
The love-spent youth, and love-sick maid,
Come to weep out the night.
1 Among thy fancies, tell me this,
What is the thing we call a kiss?
2 I shall resolve ye what it is:--
It is a creature born and bred
Between the lips, all cherry-red,
By love and warm desires fed,--
CHOR. And makes more soft the bridal bed.
2 It is an active flame, that flies
First to the babies of the eyes,
And charms them there with lullabies,--
CHOR. And stills the bride, too, when she cries.
2 Then to the chin, the cheek, the ear,
It frisks and flies, now here, now there:
'Tis now far off, and then 'tis near,--
CHOR. And here, and there, and every where.
1 Has it a speaking virtue? 2 Yes.
1 How speaks it, say? 2 Do you but this,--
Part your join'd lips, then speaks your kiss;
CHOR. And this Love's sweetest language is.
1 Has it a body? 2 Ay, and wings,
With thousand rare encolourings;
And as it flies, it gently sings--
CHOR. Love honey yields, but never stings.
Charm me asleep, and melt me so
With thy delicious numbers;
That being ravish'd, hence I go
Away in easy slumbers.
Ease my sick head,
And make my bed,
Thou Power that canst sever
From me this ill;--
And quickly still,
Though thou not kill
My fever.
Thou sweetly canst convert the same
From a consuming fire,
Into a gentle-licking flame,
And make it thus expire.
Then make me weep
My pains asleep,
And give me such reposes,
That I, poor I,
May think, thereby,
I live and die
'Mongst roses.
Fall on me like a silent dew,
Or like those maiden showers,
Which, by the peep of day, do strew
A baptism o'er the flowers.
Melt, melt my pains
With thy soft strains;
That having ease me given,
With full delight,
I leave this light,
And take my flight
For Heaven.