text:
Kevin Newcomb | October 19, 2006 | Comments
Google revenues in the third quarter rose 70 percent, and profits were up 92 percent over last year.
Google reported strong revenues for the third quarter, led by search, and expects its recent video content deals to bring more success in the future.
"Business is very, very good here. We had an excellent quarter in all respects, especially international," said Google CEO Eric Schmidt.
Google revenues reached $2.69 billion for the third quarter, up 70 percent over last year's $1.6 billion in the third quarter, and up 10 percent over last quarter's $2.46 billion. Net income for the quarter rose 92 percent from the same period last year to $733 million, or $2.36 per share; a slight increase over last quarter's $721 million, or $2.33 per share.
Revenues from outside of the United States contributed 44 percent of total revenues in the third quarter of 2006, compared to 42 percent in the second quarter of 2006 and 39 percent in the third quarter of 2005.
Google-owned sites generated 60 percent of total revenues in the quarter, or $1.63 billion, while AdSense partner sites brought in 39 percent of total revenues, or $1.04 billion. In the same period last year, Google-owned sites brought in 56 percent of revenues.
Traffic acquisition costs (TAC), or the revenues shared with Google's AdSense sites and distribution partners, increased to $825 million in the third quarter, up 56 percent from $530 million a year ago, and up 5 percent from $785 million in the second quarter. TAC as a percentage of advertising revenues fell to 31 percent in the third quarter from 34 percent a year ago, a slight dip from 32 percent in the second quarter. That number shows more of Google's revenue coming from sites it owns.
Schmidt outlined five main drivers of success in the quarter: strong user growth and improved search quality; better ad quality; diversity of Google's business; a "blizzard" of new product launches; and Google's partnerships.
Google struck several partnership deals, notably several distribution deals with major studios made right before the YouTube acquisition was announced. Many of those deals include provisions for some form of revenue sharing, according to Schmidt.
"The best partnership comes when both partners have a share in the success of those deals," Schmidt said.
Applying Google's monetization engine to quality video content from top publishers will bring success for both, Brin said. Improved ad targeting, and a growing advertiser and user base, will allow Google to come up with new ways to monetize that content, he said.
To address the confusion that began to result from so many product launches, Sergei Brin, Google's co-founder and president of technology, has begun a push inside the company to build horizontal "features" integrated into existing products instead of separate new products, he said.
"I'm concerned that if we continue to develop individual products in silos, users will have to search for the features first before they can do a search," Brin said. To that end, Google is beginning to integrate more of its search products into its general search results, he said.
Earlier this week, Yahoo reported disappointing results, and chairman and CEO Terry Semel immediately went on the offensive, accelerating the launch of its Project Panama search marketing platform, and touting Yahoo's video prowess in a clear response to Google's YouTube acquisition.
Kevin Newcomb joined ClickZ in August 2004, covering search marketing and other online marketing topics. He has been reporting on web-based businesses since 2000.
Before the bubble burst, Kevin was a marketing manager for an online computer reseller, handling copywriting, e-mail marketing, search marketing and running the affiliate program.
With a combination of real-world marketing experience and years of business journalism, Kevin brings to ClickZ a unique ability to deliver news and training materials that help online marketers do their jobs better.
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This is a group for young Nerdfighters to meet and mingle. I made this group because I noticed that the only teenage nerdfighting group hadn't been touched since '09. So..... enjoy!!!!
Location: Nerdfighteria
Members: 3046
Latest Activity: Aug 15
After seeing everyone introduce themselves on the group page I decided that we needed a place for everyone to introduce themselves and get to know those in the group....so I guess I'll start :)My…Continue
Tags: Awesome, Teens, Introductions
Started by Indira Sanchez. Last reply by martina krakora Aug 12.
Not a single cinema in my city of Lahore pakistan is playing paper towns and I and my nerdfighter friends are desperate to watch, could we somehow get this matter to John
Started by hammad. Last reply by Aparajita Jul 27.
Is is bad that I get very frustrated when I have to explain who the Nerdfightaria are and who John and Hank Green are. I need nerdfighter friends, Centralia is so obtuse.
Hey I was just wondering if there were any other nerdfighter new york city teens out there. I'm sixteen and go a an all girls school here in the city which i despise with a burning passion and am looking for someone who either a) does not reside within the confines of my social circle or b) does and reghards it with as much distaste as I do. I love Vlogbrothers, crash course and all the John Green books (especially Paper Towns {Question for the grammar nerfighters; Does the period go inside or outside the parentheses?. Also was that supposed to be a colon or a semi-colon?}) I love to have safe nerdy fun and am a little crazy. But yeah, NYC anyone?
P.S. As you can tell I suck at grammar. I happen to be a huge History and Science nerd though.
Hey guys me and some friends set up a website, please check it out :) http://ideasonpaper.co.uk
Adina, there was an episode in which it explained some of the basics of being a nerdfighter, look for that one. Also, maybe if you explain what dftba means, they'll stop asking about that one.
Hi all. iv only recently joined nerdfighters but have already become fully obsessed. im having trouble explaining to my friends and family why i am watching blogs about two brothers and saying wierd things lik dftba. could you help me? thanks:)
Good to have you around Maricruz.
Hey Everyone!!! I'm new to Nerdfighteria but I've loved John and Hank's videos since I discovered John's book TFIOS from a friend. I'm 16 and from the United States. I love to read I am a huge fan of authors like F. Scott Fitzgerald and Ray Bradbury. I love being a nerdfighter! DFTBA:)
Jorgina you sound great.
Hey Guys!! I make covers on youtube and I would appreciate if you could give this video a "like" (If you like it of course) DFTBA
I'm fine and I appreciate you all care.
© 2015 Created by Hank Green. Powered by

subset: commoncrawl


text:
Exploring bikeability in a metropolitan setting: stimulating and hindering factors in commuting route environments
1 The Research Unit for Movement, Health and Environment, The Åstrand Laboratory, GIH - The Swedish School of Sport and Health Sciences, SE-114 86 Stockholm, Sweden
2 School of Health and Medical Sciences, Örebro University, SE-701 82 Örebro, Sweden
3 Department of Health Sciences, Mid Sweden University, SE-831 25 Östersund, Sweden
BMC Public Health 2012, 12:168 doi:10.1186/1471-2458-12-168Published: 8 March 2012
Route environments may influence people's active commuting positively and thereby contribute to public health. Assessments of route environments are, however, needed in order to better understand the possible relationship between active commuting and the route environment. The aim of this study was, therefore, to assess the potential associations between perceptions of whether the route environment on the whole hinders or stimulates bicycle commuting and perceptions of environmental factors.
The Active Commuting Route Environment Scale (ACRES) was used for the assessment of bicycle commuters' perceptions of their route environments in the inner urban parts of Greater Stockholm, Sweden. Bicycle commuters (n = 827) were recruited by advertisements in newspapers. Simultaneous multiple regression analyses were used to assess the relation between predictor variables (such as levels of exhaust fumes, noise, traffic speed, traffic congestion and greenery) and the outcome variable (hindering - stimulating route environments). Two models were run, (Model 1) without and (Model 2) with the item traffic: unsafe or safe included as a predictor.
Overall, about 40% of the variance of hindering - stimulating route environments was explained by the environmental predictors in our models (Model 1, R2 = 0.415, and Model 2, R2= 0.435). The regression equation for Model 1 was: y = 8.53 + 0.33 ugly or beautiful + 0.14 greenery + (-0.14) course of the route + (-0.13) exhaust fumes + (-0.09) congestion: all types of vehicles (p ≤ 0.019). The regression equation for Model 2 was y = 6.55 + 0.31 ugly or beautiful + 0.16 traffic: unsafe or safe + (-0.13) exhaust fumes + 0.12 greenery + (-0.12) course of the route (p ≤ 0.001).
The main results indicate that beautiful, green and safe route environments seem to be, independently of each other, stimulating factors for bicycle commuting in inner urban areas. On the other hand, exhaust fumes, traffic congestion and low 'directness' of the route seem to be hindering factors. Furthermore, the overall results illustrate the complexity of a research area at the beginning of exploration.


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$7.99Jocelyn Butler has been hiding from her past for years. But all her secrets are about to be laid bare… Four years ago, Jocelyn left her tragic past behind in the States and started over in Scotland, burying her grief, ignoring her demons, and forging ahead without attachments. Her solitary life is working well—until she moves into a new apartment on Dublin Street where she meets a man who shakes
Valentine: An On Dublin Street NovellaJoss & Braden. Ellie & Adam. Jo & Cam. Olivia & Nate. Hannah & Marco. Shanon & Cole. And one Valentine's Day they'll never forget.It's Valentine's Day! How do Joss & Braden, Ellie & Adam, Jo & Cam, Olivia & Nate, Hannah & Marco and Shannon & Cole keep the fire burning in their relationships while trying to juggle busy careers and the joyful chaos of parenthood? Find out in this special novella
$5.99From the New York Times bestselling author of On Dublin Street comes a seductive story of forgiving the past and making up for lost time.…Shannon MacLeod has always gone for the wrong type of man. After she drifted from one toxic relationship to the next, her last boyfriend gave her a wake-up call in the worst possible way. With her world shattered, she's sworn off men—especially those of the bad
$5.99Logan from Echoes of Scotland Street is back with his own smoldering story, as the New York Times bestselling On Dublin Street series returns…Logan spent two years paying for the mistakes he made. Now, he's ready to start over. He has a great apartment, a good job, and plenty of women to distract him from his past. And one woman who is driving him to distraction…Grace escaped her manipulative
$3.99The New York Times bestselling author of On Dublin Street returns to the bar where it all began with Braden and Joss for a white-hot new novella full of passion and drama. When he's not working at the club, Craig Lanaghan looks out for his mother and little sisters. So when it comes to women, all he wants is a good time. But once Rain Alexander walks into his life, there's no denying that this
The House on Parkgate Street and Other Dublin StoriesA child wandering alone through a racecourse; an elderly lady grasping at a thread of memory; a young girl watching prostitutes from her window as they ply their trade; an Indian taxi driver living in his cab: these are some of the fractured lives and fragile hearts we meet in The House on Parkgate Street and Other Dublin Stories. Set in various eras, at various times of life, each story is a
The High-Street Bride's Guide: How to Plan Your Perfect Wedding On A BudgetSamantha Birch has written for GLAMOUR, Brides, You & Your Wedding and Cosmopolitan Bride. She knows a thing or two about planning a wedding on a budget, how much you can expect to pay for everything and where to go to get it for less. And she's put it all down here. You can say your vows in a catwalk gown so beautiful it reduces your mum to tears (and not because she paid for it). You can style a


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Hôtel Saint Jean, Wimereux
- Free WiFi
Main amenities
- 24 guestrooms
- Near the beach
- Breakfast available
- Spa tub
- Bar/lounge
- Sauna
- Self parking
- 24-hour front desk
- Tour/ticket assistance
What's around
- Heart of Wimereux
- Wimereux Golf (1.6 mi / 2.6 km)
- Colonne de la Grande Armee (2.4 mi / 3.9 km)
- Nausicaa (3.6 mi / 5.8 km)
- Basilique Notre-Dame (4.1 mi / 6.6 km)
excellent budget hotel ideal for overnight stop before or after ferry crossing.…
Good, friendly, small hotel in a pleasant town.
Enter dates to check prices
Hotel Info: 020 3027 9808from £56
- Double Room
- Single Room
- Twin Room
- Triple Room
- Quadruple Room
Also popular with travellers from the UK
At a glance
Key facts
Hotel size
- This hotel has 24 rooms
- This hotel is arranged over 3 floors
- Check-in time starts at 3:30 PM
- Check-out time is noon
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Pets allowed (dogs only) *
- Free WiFi in public areas
- Free WiFi in rooms
- Self parking *
- Accessible parking
Nearby Three Star Wimereux hotels
In the hotel
- Continental breakfast daily (surcharge)
- Bar/lounge
- 24-hour room service
- Spa tub
- Sauna
- 24-hour front desk
- Tours/ticket assistance
- Number of buildings/towers - 1
- Elevator/lift
- Disabled parking
- Accessibility equipment for the deaf
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- Climate control
- Soundproofed rooms
- Sleep Number by Select Comfort mattress
- Private bathroom
- Bathtub or shower
- Hair dryer
- Satellite TV channels
- Desk
- Free WiFi
- Phone
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Mandatory fees
You'll be asked to pay the following charges at check in or check out:
- A tax is imposed by the city: EUR 1 per person, per night
Optional extras
Self parking costs EUR 8 per night
Continental breakfast is offered for an extra charge of EUR 12 for adults and EUR 7 for children (approximately)
Pets are allowed for an extra charge of EUR 14 per pet, per day
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


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1111 Model
AmberfetteModel Female Toronto, Ontario, Canada
My Website: Amberfette on Deviant Art
Mayhem # 195726
About Me
I often model for photographers updating their portfolios, for hair contest photo shoots and have been used on a flyer. I also have an interest in photography, as it is one of my hobbies and passions, but I do not photograph people. I prefer taking photos of places, things, and abstract creative photography. I am not here to find people to photograph. My style ranges, but I am usually used for the punk/rock/gothish style. I have a very unique look and am quite the character.
I am open to working with all kinds of photographers and styles.
Contact me through this site if you have any interest in working with me.
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Our new "How to Study the Bible" series starts soon! Sign up today.
(beginnings), one of the towns in the district east of the Dead Sea allotted to the tribe of Reuben, (Joshua 13:18) given by the Merarite Levites. (Joshua 21:37; 1 Chronicles 6:79) It possibly conferred its name on the "wilderness," or uncultivated pasture land, "of Kedemoth." (Numbers 21:33; 2:26,27) etc.


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Spanish Prisoner
A Con Man identifies a potential mark—someone with wealth and naivete. The con man convinces the mark that he serves a dethroned princess who is being held prisoner in, say, Spain. If the mark can come up with just a few hundred dollars, then a guard can be bribed and the princess can flee to the US (where the mark lives) in eternal gratitude. The mark can easily part with a few hundred, and so, though he is wary, he falls far enough for the con man's smooth line. A week goes by. Two. The mark has come to understand that he's been tricked, but before that last spark of hope can die, the con man reappears with a letter from Her Highness. She is free and in France. Now she needs a few thousand dollars for her final passage by sea, and the mark gladly shells it out. Next, the princess might need money to bail out her mother and father, the ex-monarchs; then, she'll need to buy off a Spanish spy who has discovered her escape. The mark keeps paying for as long as the con artists can keep him fooled. The con man shows the mark photographs of the beautiful princess (who is really Dotty from the Nighthawk Diner in a ten-cent tiara). More players are brought in to act out additional roles, each earning part of the take. Finally, the mark can be brushed off. One way to do this is for the princess to arrive, at last, on the mark's doorstep. The con man is there, too, but then the Spanish spy shows up and murders the con man. (It's an act, of course; the con man usually has a "cackle bladder" full of fake blood in his mouth, and he usually makes sure to get some of it on the mark's clothes as he falls.) The mark is terrified, and the princess runs to his side, kisses him tenderly, and tells him that she must go into exile or she will endanger his life as well as her own. And so, the mark never sees the princess again, and the con man, princess, and Spanish spy agent all split the take. In this way the Mark never realizes he was conned and doesn't go to the police. There are real-life examples of marks who discovered the con only when they were approached by the police and asked to testify. That's the classic Spanish Prisoner. Today's updates are called Russian Bride and Save This Hooker. It's one of The Oldest Tricks in the Book. A variant frequently encountered on the Internet these days is the "419 Scam" (named after the relevant section of the Nigerian banking code, where some of these scams originate), in which the "prisoner" is replaced by a relative of some recently deceased Third World dictator, who asks the potential mark to help him smuggle and/or launder stolen UN aid money. Or get bank boxes out of a third country. Or steal the ill-gotten windfall profit of an over-invoiced oil contract. If you're reading this, you've probably received spam about it yourself. The total amount of over-invoiced oil on all of these supposed "lucrative" contracts probably exceeds the output of Saudi Arabia for ten years. While the two tropes are similar, in works the Spanish Prisoner scam is much more likely to drive the whole plot, while Four One Nine Scams are often used as a bit of characterization or as a joke.
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- A variation of this scam is the main modus operandi of Michael Caine's character in Dirty Rotten Scoundrels. Michael Caine generally plays the Prince himself, and is in exile already (though in desperate needs of funds to finance La Résistance back home) when he meets the mark (usually an unsophisticated, rich, FEMALE, American tourist). When Steve Martin comes on as Caine's apprentice of sorts, Martin takes on the role of the Prince's "special" brother to drive the mark away of her own volition once the money has been obtained. Thus, any unseemly violence (fake though it would be) is avoided entirely. (Caine is almost caught in one scene in the movie where he is recognized by a former mark while working on another one, using another identity, and has to use some fast thinking to prevent both from getting suspicious.)
- Cleverly, David Mamet's The Spanish Prisoner includes a detailed explanation of this con, the purpose of which exposition is to disguise the fact that it is not the con being performed.
- A particularly stellar Renaissance-esque example appears in The Lies Of Locke Lamora by Scott Lynch, with a large shipment of soon-to-be rare brandy standing in for the prisoner. The big, wonderful twist? Halfway through the con the con men dress up as secret agents for the government, explain that the brandy merchant is a notorious con man and advise the mark to continue to give them money while the authorities move in to catch them. Naturally they advise the mark to tell no one about this, lest the "con men" suspect something and run. That twist is a variation on a common scam. Once you have figured out that you can take the mark once, send in accomplices who let the mark think they are getting the con men arrested.
- In Neil Gaiman's American Gods, Mr. Wednesday names this con as an old favorite of his, though he never describes it in detail.
- An Inspector Morse mystery centered around the Russian Bride version of this scam.
Live Action TV
- Leverage, "The Stork Job" - the mark of the week is in with the Russian Mafia, and runs a version using Serbian war orphans where the prospective parents have to keep paying "overhead" costs. Sophie explicitly compares the scheme to the Spanish Prisoner.
- A That Mitchell and Webb Look episode featured three millionaires (all suffering from a speech impediment that made them sound exactly like automated call generators) who actually were handing out massive yachts for just a small fee to people whose phone number had been selected at random. They just did not understand why everyone kept hanging up on them.
- Hustle - a few times over the course of the series, but most notably in season 7, they pulled this off comfortably against a new mark they'd not been expecting AND against the other three marks they'd had on the hook at the beginning of the episode, but who had not since been mentioned, earning them a triumphant record of pulling off the Spanish Prisoner against more marks in one day than any other crew.
- Burn Notice has one guy taken in by an interesting version of this particular con: he's convinced to invest in a new club in Cuba (highly illegal for US residents), necessitating a cash-only transaction. He's convinced by profits shown from an earlier investment (actually a Ponzi Scheme) and just when he's called to meet with the investor to discuss profits, the FBI conducts a sting operation, hustling him out the back door while they arrest the investor. Of course, the "FBI" was in on the plot, so he asks Michael to step in and get his money back (lest he incur the wrath of the loan sharks he got the money from in the first place), so Michael pulls a more complicated version of the same scheme on the con man.
- Community has Abed's internet friend Toby, a banker in Nigeria experiencing financial difficulties and requiring Abed to send him $700 to enable him to fly out of the country to meet him. After Abed explains this, Britta is just about to explain that Abed has fallen for one of these... when Toby shows up, thanks Abed, pays him back the $700 and complains how Abed was the only one to actually help him out.
- Message No. 419 by MC Frontalot is about the rapper receiving such an email scam, and then turning it around on the scammer.


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Bloody Coochie
Mixing instructions:
Fill glass half full of ice Pour in vodka, triple sec, sour mix, OJ, and fruit punch Stir, or shake
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Page 1
EUKARYOTIC CELL, Nov. 2005, p. 1794–1800
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Vol. 4, No. 11
Cyclic AMP-Independent Regulation of Protein Kinase A Substrate
Phosphorylation by Kelch Repeat Proteins
Ailan Lu and Jeanne P. Hirsch*
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029
Received 27 July 2005/Accepted 27 August 2005
Pseudohyphal and invasive growth in the yeast Saccharomyces cerevisiae is regulated by the kelch repeat-
containing proteins Gpb1p and Gpb2p, which act downstream of the G protein ?-subunit Gpa2p. Here we show
that deletion of GPB1 and GPB2 causes increased haploid invasive growth in cells containing any one of the
three protein kinase A (PKA) catalytic subunits, suggesting that Gpb1p and Gpb2p are able to inhibit each of
these kinases. Cells containing gpb1? gpb2? mutations also display increased phosphorylation of the PKA
substrates Sfl1p and Msn2p, indicating that Gpb1p and Gpb2p are negative regulators of PKA substrate
phosphorylation. Stimulation of PKA-dependent signaling by gpb1? gpb2? mutations occurs in cells that lack
both adenylyl cyclase and the high-affinity cyclic AMP (cAMP) phosphodiesterase. This effect is also seen in
cells that lack the low-affinity cAMP phosphodiesterase. Given that these three enzymes control the synthesis
and degradation of cAMP, these results indicate that the effect of Gpb1p and Gpb2p on PKA substrate
phosphorylation does not occur by regulating the intracellular cAMP concentration. These findings suggest
that Gpb1p and Gpb2p mediate their effects on the cAMP/PKA signaling pathway either by inhibiting the
activity of PKA in a cAMP-independent manner or by activating phosphatases that act on PKA substrates.
Activation of protein kinase A (PKA) by cyclic AMP
(cAMP) is a conserved feature of eukaryotic signaling systems.
In the yeast Saccharomyces cerevisiae, PKA regulates cell
growth and morphology in response to nutrient and stress
signals (31). Yeast PKA functions downstream of the mono-
meric G proteins Ras1p and Ras2p, which are activated by an
unknown mechanism (3). Ras1p and Ras2p stimulate adenylyl
cyclase to produce cAMP (8, 9, 35), which activates PKA
through the well-established mechanism of binding to the reg-
ulatory subunit of PKA and releasing active catalytic subunits
(33). There are three isoforms of the catalytic subunit, called
Tpk1p, Tpk2p, and Tpk3p, which are encoded by different
genes (34). Deletion of the three genes encoding the catalytic
subunits is lethal, but any one of the three genes can provide
the essential function of PKA. There is one regulatory subunit,
called Bcy1p, which is thought to bind to each of the three
catalytic subunits (33). Activation of PKA results in phosphor-
ylation of substrates that are involved in intermediary metab-
olism, stress responses, and filamentous growth (10, 31).
Yeast PKA also appears to function downstream of the G
protein ?-subunit Gpa2p (5, 17, 21). Gpa2p is coupled to a cell
surface receptor, called Gpr1p, that contains seven membrane-
spanning domains, a structure that is found in other G protein-
coupled receptors (16, 36, 37). The effects conferred by a
deletion of GPR1 are suppressed by constitutive activation of
Gpa2p, as would be expected for a G protein that acts down-
stream of its coupled receptor (22, 30). In contrast to the
situation with Ras proteins, little is known about the way in
which the Gpa2p pathway regulates cAMP/PKA signaling. The
effect of Gpa2p on PKA responses has recently been shown to
involve the kelch repeat-containing proteins Gpb1p and
Gpb2p (also called Krh2p and Krh1p, respectively) (1, 14).
Gpb1p and Gpb2p physically interact with Gpa2p, suggesting
that they function in the signaling pathway. Deletion of GPB1
and GPB2 results in phenotypes that are characteristic of in-
creased PKA signaling, indicating that Gpb1p and Gpb2p in-
hibit a component of the cAMP/PKA pathway. Gpb1p and
Gpb2p appear to transmit the signal generated by Gpa2p to
downstream components, because gpb1? gpb2? mutations sub-
stantially suppress the defects in pseudohyphal and invasive
growth conferred by a gpa2? mutation. Gpb1p and Gpb2p
appear to act upstream of PKA, because the increase in sig-
naling conferred by gpb1? gpb2? mutations is substantially
blocked by deletion of TPK2, which encodes a PKA catalytic
isoform. These results are consistent with a model in which
activation of Gpa2p relieves the inhibition of PKA that is
either directly or indirectly mediated by Gpb1p and Gpb2p,
resulting in increased PKA activity.
Here we show that Gpb1p and Gpb2p affect signaling by
altering the level of phosphorylation of PKA substrates. How-
ever, the function of Gpb1p and Gpb2p does not require
changes in the intracellular concentration of cAMP. These
results imply that the signaling process initiated by the Gpa2p
?-subunit does not constitute a linear pathway that acts solely
by stimulating the production of cAMP but rather has at least
one component that acts downstream of adenylyl cyclase.
Plasmid construction. To construct a HIS3 disruption of TPK1, a 1.7-kb XbaI
fragment from pHIS3-Bs.1 was cloned into the XbaI sites of plasmid pTPK1-
50.1, which consists of vector YCp50 with a 1.7-kb insert containing the TPK1
gene, to produce plasmid ptpk1-1::HIS3. To construct a HIS3 disruption of
TPK2, a 1.7-kb EcoRI-NotI fragment from pHIS3-Bs.2 was cloned into the
EcoRI-NotI sites of plasmid pTPK2N-Bs.1 to produce plasmid ptpk2-2::HIS3.
Plasmid pTPK2N-Bs.1 consists of a 2.0-kb BglII fragment containing the TPK2
gene cloned into the BamHI site of pBluescript, in which a NotI site was inserted
* Corresponding author. Mailing address: Department of Pharma-
cology and Biological Chemistry, Mount Sinai School of Medicine, Box
1603, 1 Gustave L. Levy Place, New York, NY 10029. Phone: (212)
241-0224. Fax: (212) 996-7214. E-mail: firstname.lastname@example.com.
Page 2
before the stop codon by site-directed mutagenesis. To construct a HIS3 disrup-
tion of TPK3, a 1.7-kb EcoRI-XhoI fragment from pHIS3-Bs.1 was cloned into
the EcoRI-XhoI sites of a plasmid with a 2.7-kb HindIII fragment containing the
TPK3 gene to produce plasmid ptpk3-3::HIS3. Plasmid pCYR1.Bs contains a
4.5-kb KpnI-XbaI fragment from pCYR1 cloned into the KpnI-XbaI sites of
pBluescript. To construct a URA3 disruption of CYR1, a 1.0-kb HindIII-NsiI
fragment from pURA3.Bs (1) was cloned into the HindIII-NsiI sites of plasmid
pCYR1.Bs to produce pcyr1::URA3.Bs.
Strain construction and media. Strains used in the present study are listed
in Table 1. Construction of strains containing the pde2::HIS3 allele (36) and
the gpb1::URA3 and gpb2::HIS3 alleles (1) was described previously. The
tpk1-1::HIS3 allele was made by transformation of cells with the 2.4-kb EcoRV-
AatII fragment from plasmid ptpk1-1::HIS3. The tpk2-2::HIS3 allele was made
by transformation of cells with the 2.6-kb SacI-SalI fragment from plasmid
ptpk2-2::HIS3. The tpk3-3::HIS3 allele was made by transformation of cells with
the 2.7-kb NcoI-EcoRV fragment from plasmid ptpk3-3::HIS3. The gpb2::TRP1
allele was made by transformation of a gpb2::HIS3 strain with a 3.8-kb SmaI/
XhoI fragment from marker swap plasmid pHT6 (7). The cyr1::URA3 allele was
made by transformation of cells with the 2.9-kb KpnI-XbaI fragment from plas-
mid pcyr1::URA3.Bs. The cyr1::TRP1 allele was made by transformation of cells
with the 3.7-kb SmaI fragment from marker swap plasmid pUT11 (7). The
pde1::kanMX allele was made by transformation of cells with a pde1::kanMX
cassette amplified from genomic DNA of a strain containing this allele (Open
Biosystems). Strain constructions involving transformations were confirmed by
Southern blot or PCR.
Strains were grown on YEPD (yeast extract-peptone-dextrose) with 2% glu-
cose, and strains under selection were grown on synthetic dropout media, as
described previously (13).
Yeast methods and protein assays. Invasive growth assays were performed
according to the method of Kuchin et al. (18). Yeast transformations were
performed by the lithium acetate method according to standard procedures (13).
Cell lysates for immunoblots were prepared from cells growing in log phase
(optical density at 600 nm ? 0.5 to 0.85). Cells were washed once with cold water
and resuspended in lysis buffer, made essentially as described previously (27),
with the exception that 25 mM ?-glycerophosphate was substituted by 15 mM
p-nitrophenyl phosphate. Cells were disrupted by shaking in the presence of glass
beads for 5 min at 4°C and cleared by centrifugation for 3 min. For immunoblots,
proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electro-
phoresis (SDS-PAGE) and transferred to nitrocellulose. Blots were probed with
the following antibodies: anti-myc 9E10 at a dilution of 1/1,000, anti-phospho-
CREB (Ser133) (Cell Signaling) at a dilution of 1/1,000, and anti-green fluores-
cent protein (GFP) serum (Molecular Probes) at a dilution of 1/1,000. Secondary
antibodies were donkey anti-rabbit immunoglobulin conjugated to horseradish
peroxidase (Amersham) at a dilution of 1/10,000 and goat anti-mouse immuno-
globulin conjugated to horseradish peroxidase (Amersham) at a dilution of
1/10,000. Immune complexes were detected with an ECL or an ECL Plus kit
Heat shock assays and yeast RNA extraction were performed as described
previously (36) except that cells were incubated for 15 min at 50°C. Northern
blots were performed as described previously (1).
Effect of gpb1? gpb2? mutations is not specific to Tpk2p.
Deletion of GPB1 and GPB2 causes an increase in diploid
pseudohyphal growth and haploid invasive growth, phenotypes
that are characteristic of increased activation of the cAMP/
PKA signaling pathway (1, 14). It was therefore of interest to
determine which pathway component is the target of inhibition
by Gpb1p and Gpb2p. In yeast, total PKA activity results from
the combined activities of the three catalytic subunits of
cAMP-dependent kinase, Tpk1p, Tpk2p, and Tpk3p (34). To
test the effects of gpb1? gpb2? mutations on the individual
PKA catalytic isoforms, phenotypes associated with filamen-
tous growth were investigated in strains in which two of the
three TPK genes were deleted. Previous results have shown
that TPK2 has a positive effect on filamentous growth and that
TPK1 and TPK3 have negative effects on filamentous growth
when TPK2 is present (26, 28). However, in cells containing
either TPK1 only or TPK3 only, a small but reproducible in-
crease in invasive growth was observed in gpb1? gpb2? cells
compared to wild-type cells (Fig. 1A). The most straightfor-
ward interpretation of these results is that TPK1 and TPK3
have a modest positive effect on invasive growth in the absence
of TPK2 and that GPB1 and GPB2 inhibit that effect. Expres-
sion of FLO11, which encodes a cell surface flocculin that is
required for both pseudohyphal and invasive growth (20), was
undetectable in strains containing either TPK1 only or TPK3
only (Fig. 1B, lanes 3, 4, 7, and 8), indicating that the signal
generated in cells lacking TPK2 is quite low. Cells containing
TPK2 only display very high levels of invasive growth and
FLO11 RNA that were not affected by gpb1? gpb2? mutations
(Fig. 1A and B, lanes 5 and 6). In this strain, the high level of
Tpk2p activity is due to lack of inhibition by Tpk1p and Tpk3p.
However, detectable FLO11 RNA abundance in wild-type cells
depends on TPK2, and gpb1? gpb2? mutations increase the
level of FLO11 RNA in wild-type cells (Fig. 1B, lanes 1 and 2).
These results imply that the presence of GPB1 and GPB2
causes a decrease in Tpk2p activity. In summary, these findings
are consistent with the idea that GPB1 and GPB2 are able to
inhibit each of the PKA catalytic isoforms.
TABLE 1. Strains used in this study
MATa ura3-52 trp1::hisG leu2::hisG his3::hisG
MATa pde1::kanMX
MATa gpb1::URA3 gpb2::HIS3
MATa gpb1::URA3 gpb2::HIS3 pde1::kanMX
MATa tpk2-2::HIS3
MATa gpb1::URA3 gpb2::HIS3 tpk2-2::HIS3
MAT? cyr1::URA3 pde2::HIS3
MAT? cyr1::TRP1 pde2::HIS3
MAT? gpb1::URA3 gpb2::HIS3 cyr1::URA3 pde2::HIS3
MATa gpb1::URA3 gpb2::HIS3 tpk1-1::TRP1 tpk3-3::HIS3
MATa tpk1-1::TRP1 tpk3-3::HIS3
MATa gpb1::URA3 gpb2::HIS3 tpk2-2::TRP1 tpk3-3::HIS3
MATa gpb1::URA3 gpb2::HIS3 tpk1-1::HIS3 tpk2-2::TRP1
MATa tpk2-2::TRP1 tpk3-3::HIS3
MATa tpk1-1::HIS3 tpk2-2::TRP1
S. Palecek and S. Kron
This study
This study
This study
This study
This study
This study
This study
This study
This study
This study
This study
This study
This study
This study
aStrains with a SKY762 background are indicated with an asterisk. SKY762 is derived from ?1278b (19).
VOL. 4, 2005Gpb1p AND Gpb2p CONTROL PKA ACTIVITY1795
Page 3
PKA activity is increased in gpb1? gpb2? cells. The exper-
iments described above indicate that strains containing gpb1?
gpb2? mutations display phenotypes that are characteristic of
increased activation of PKA. However, these experiments do
not demonstrate whether gpb1? gpb2? mutations affect the
activity of PKA itself. The results are also consistent with the
possibility that gpb1? gpb2? mutations affect the activity of a
related kinase that regulates the same physiological processes
as PKA, such as Sch9p (32). To test directly whether Tpk2p
activity is affected by the presence of Gpb1p and Gpb2p, the
phosphorylation state of a Tpk2p substrate was examined in
different mutant backgrounds.
Expression of FLO11 is regulated by the transcriptional re-
pressor Sfl1p, which is thought to be an in vivo substrate of
Tpk2p based on the following observations. Sfl1p acts down-
stream of Tpk2p because deletion of SFL1 restores FLO11
expression in tpk2? mutant cells (27, 28). Sfl1p binds specifi-
cally to Tpk2p, and in vitro phosphorylation of Sfl1p by Tpk2p
inhibits its binding to the FLO11 promoter (6, 27, 28). Finally,
phosphorylation of Sfl1p in vivo requires the presence of
Tpk2p (27). Phosphorylation of Sfl1p by Tpk2p can be de-
tected as a change in mobility on a gel (6, 27, 28). In wild-type
cells, deletion of GPB1 and GPB2 results in a significant in-
crease in the phosphorylated form of Sfl1p (Fig. 2A, lanes 1
and 2). There was no detectable phosphorylated Sfl1p in
cells containing a tpk2? mutation, and deletion of GPB1 and
GPB2 had no effect in this mutant background (Fig. 2A,
lanes 3 and 4).
One phenotype that is affected by all three forms of PKA is
the response to stress, which is mediated, in part, by the PKA
substrate Msn2p. Msn2p is a stress-activated transcription fac-
tor that is negatively regulated by PKA phosphorylation (11,
29). Msn2p is phosphorylated on serine 620 by PKA, and the
phosphorylated epitope is recognized by an antibody specific
for the phosphorylated form of mammalian CREB (12).
Strains transformed with a construct expressing an Msn2p-
GFP fusion protein were used to assay the level of Msn2p
phosphorylation in different mutant backgrounds by compar-
ing the signal obtained with the anti-phospho-CREB antibody
to that obtained with an anti-GFP antibody. A substantial
increase in the level of Msn2p phosphorylation at serine 620
was observed in cells containing gpb1? gpb2? mutations com-
pared to wild-type cells (Fig. 2B, lanes 1 and 2). A pronounced
effect on Msn2p phosphorylation was also seen in cells
containing an activated allele of RAS2, which is known to
increase PKA activity by stimulation of adenylyl cyclase activity
(Fig. 2B, lanes 3 and 4). These results establish that gpb1?
FIG. 1. PKA activity is increased by gpb1? gpb2? mutations.
(A) The following strains were patched onto YEPD–2.5% agar me-
dium, incubated for 4 days at 25°C, and photographed before (Total
growth) and after (Invasive growth) rubbing the surface of the plate with
a glass rod under a stream of water: wild-type strain SKY762, gpb1?
gpb2? strain HS182-3B, tpk1? tpk2? strain HS234-1C, gpb1? gpb2?
tpk1? tpk2? strain HS232-2B, tpk1? tpk3? strain HS230-15B, gpb1?
gpb2? tpk1? tpk3? strain HS229-11D, tpk2? tpk3? strain HS233-8B,
and gpb1? gpb2? tpk2? tpk3? strain HS231-29A. (B) RNA isolated
from the log-phase cultures of the strains described in panel A was
transferred to nylon membrane, hybridized with a FLO11 probe, and
rehybridized with an ACT1 probe.
FIG. 2. Phosphorylation of in vivo PKA substrates is increased by
gpb1? gpb2? mutations. (A) Extracts made from the following strains
expressing Sfl1p-myc from plasmid pXP181 (27) (provided by X. Pan
and J. Heitman) were analyzed by SDS-PAGE and immunoblotting
with anti-myc antibody 9E10: wild-type strain SKY762 (lane 1), gpb1?
gpb2? strain HS182-3B (lane 2), tpk2? strain HS192-2C (lane 3), and
gpb1? gpb2? tpk2? strain HS196-5A (lane 4). (B) Extracts made from
the following strains expressing Msn2p-GFP fusion protein from plas-
mid pAdh1-Msn2-GFP (12) (provided by C. Schu ¨ller and W. Reiter)
were analyzed by SDS-PAGE and immunoblotting with antibodies to
P-CREB (S133) and GFP: wild-type strain SKY762 (lane 1), gpb1?
gpb2? strain HS182-3B (lane 2), SKY762 carrying vector YCplac33
(lane 3), and SKY762 carrying plasmid YCp50-RAS2ala18val19(lane 4).
Page 4
gpb2? mutations cause an increase in phosphorylation of PKA
substrates in vivo at sites that are specifically phosphorylated
by PKA.
Effect of gpb1? gpb2? mutations is independent of adenylyl
cyclase and the high- and low-affinity cAMP phosphodiester-
ases. The observation that gpb1? gpb2? mutations affect the
phosphorylation level of PKA substrates raises the question of
whether Gpb1p and Gpb2p function by inhibiting the activity
of adenylyl cyclase. Adenylyl cyclase activates PKA by produc-
ing cAMP, which binds to the PKA regulatory subunit, result-
ing in the release of active PKA catalytic subunits. The PKA
regulatory subunit, Bcy1p, is common to all three PKA cata-
lytic isoforms (33). Therefore, if Gpb1p and Gpb2p were to
function by inhibiting production of cAMP, all three PKA
catalytic isoforms would be expected to be affected by deletion
of GPB1 and GPB2. To test this idea directly, the effect of
deleting GPB1 and GPB2 was determined in a strain contain-
ing a deletion of the CYR1 gene, which encodes adenylyl cy-
clase. The viability of cells containing a cyr1? mutation can be
maintained by deletion of PDE2, a gene encoding a high-
affinity cAMP phosphodiesterase (2). In these cells, viability is
maintained by supplementing the medium with exogenous
cAMP. Cells containing cyr1? pde2? mutations were viable
when supplied with 1.5 mM cAMP but did not grow on 0.75 or
0.5 mM cAMP (Fig. 3, top panels). In contrast, cells lacking
adenylyl cyclase and carrying gpb1? gpb2? mutations were
viable on 0.75 mM cAMP. However, cyr1? pde2? gpb1? gpb2?
cells were inviable on 0.5 mM cAMP. Wild-type and pde2?
cells were viable at all concentrations of cAMP (data not
shown). Therefore, gpb1? gpb2? mutations do not confer via-
bility to cells containing very low levels of cAMP but are able
to remediate the loss of viability at an intermediate concentra-
tion of cAMP. cyr1? pde2? cells containing either an activated
allele of RAS2 or empty vector were viable when supplied with
1.5 mM cAMP but did not grow on 0.75 mM or 0.5 mM cAMP
(Fig. 3, bottom panels). Therefore, activation of Ras has no
effect in cells lacking adenylyl cyclase, a finding consistent with
previous results showing that Ras2p directly activates adenylyl
cyclase (35). The finding that deletion of GPB1 and GPB2 has
an effect on cells that cannot synthesize cAMP eliminates the
possibility that the inhibitory function of Gpb1p and Gpb2p
acts solely on adenylyl cyclase.
The level of cAMP in cells results from the combined effect
of adenylyl cyclase-mediated synthesis of cAMP and phos-
phodiesterase-mediated degradation of cAMP. The results
shown above demonstrate that gpb1? gpb2? mutations have an
effect on cells that lack adenylyl cyclase and the high-affinity
cAMP phosphodiesterase Pde2p. In addition to these enzymes,
the level of cellular cAMP is regulated by the low-affinity
cAMP phosphodiesterase Pde1p (23). To test the involvement
of Pde1p in mediating the effect of the kelch repeat proteins,
strains containing a pde1? mutation in combination with other
mutations that affect cAMP levels were constructed. However,
strains containing cyr1? pde1? pde2? mutations grew ex-
tremely slowly in several different cAMP concentrations, and
they rapidly generated faster growing variants that were likely
to contain suppressor mutations. In addition, strains contain-
FIG. 3. Effect of gpb1? gpb2? mutations is independent of adenylyl
cyclase and the high-affinity cAMP phosphodiesterase. Tenfold dilu-
tions of the following strains were spotted onto medium containing the
indicated concentrations of cAMP: HS222-3A (cyr1? pde2?) and
HS224-3D (gpb1? gpb2? cyr1? pde2?) grown in synthetic complete
medium containing 2 mM cAMP (upper panel), and HS222-3A.T
pde2?) carrying vector
RAS2ala18val19grown in medium lacking uracil and containing 2 mM
cAMP (lower panel). Plates were photographed after 2 days of growth.
YEp352or plasmid YCp50-
FIG. 4. Effect of gpb1? gpb2? mutations is independent of the
low-affinity cAMP phosphodiesterase. (A) Extracts made from the
following strains expressing Msn2p-GFP fusion protein from plasmid
pAdh1-Msn2-GFP were analyzed by SDS-PAGE and immunoblotting
with antibodies to P-CREB (S133) and GFP: wild-type strain SKY762
(lane 1), gpb1? gpb2? strain HS182-3B (lane 2), pde1? strain SKY.p1k
(lane 3), gpb1? gpb2? pde1? strain HS182-3B.p1k (lane 4). (B) The
strains described in panel A were grown to saturation for 2 days in
synthetic complete medium, incubated at 50°C for 15 min, and diluted
and plated to determine the percent survival. Values for GPB1 GPB2
strains are represented by the open bars; values for gpb1? gpb2?
strains are represented by the filled bars. Values shown are the mean
and standard deviation from three independent experiments.
VOL. 4, 2005Gpb1p AND Gpb2p CONTROL PKA ACTIVITY 1797
Page 5
ing cyr1? pde1? mutations were inviable at all concentrations
of cAMP tested (data not shown). Therefore, the effect of
gpb1? gpb2? mutations was tested in a strain containing only a
pde1? mutation.
In strains containing a pde1? mutation, Msn2p phosphory-
lation at serine 620 was substantially increased in gpb1? gpb2?
cells compared to GPB1 GPB2 cells (Fig. 4A, lanes 3 and 4).
This effect is similar to that conferred by gpb1? gpb2? muta-
tions in a wild-type strain (Fig. 4A, lanes 1 and 2). Therefore,
Pde1p is not required for the decrease in Msn2p phosphory-
lation caused by the presence of Gpb1p and Gpb2p. To deter-
mine whether PDE1 is involved in the effect of gpb1? gpb2?
mutations on a physiological phenotype that is controlled by
PKA, a heat shock experiment was performed. Deletion of
GPB1 and GPB2 results in a very large increase in heat shock
sensitivity in a wild-type background (1). In strains containing
a pde1? mutation, the effect of gpb1? gpb2? mutations on heat
shock sensitivity was the same as that seen in wild-type strains
(Fig. 4B). These results demonstrate that the inhibitory func-
tion of Gpb1p and Gpb2p does not act solely on the low-affinity
cAMP phosphodiesterase Pde1p.
The kelch repeat-containing proteins Gpb1p and Gpb2p are
negative regulators of the cAMP/PKA pathway that were iden-
tified on the basis of their interaction with the G protein
?-subunit Gpa2p (1, 14). Here we show that deletion of GPB1
and GPB2 causes an increase in the phosphorylation level of
physiologically relevant PKA substrates. These effects corre-
late with changes in PKA-dependent characteristics such as
invasive growth and heat shock sensitivity. However, the in-
crease in signaling conferred by gpb1? gpb2? mutations does
not require any of the three enzymes known to be involved in
cAMP metabolism, which include adenylyl cyclase and the
high- and low-affinity cAMP phosphodiesterases. The most
straightforward interpretation of these results is that Gpb1p
and Gpb2p either directly or indirectly inhibit a component of
the cAMP/PKA pathway that acts downstream of cAMP. In
fact, signaling through Gpb1p and Gpb2p could account for
the observation that there is a cAMP-independent mechanism
for regulating PKA-dependent phenotypes (4). Potential tar-
gets of Gpb1p and Gpb2p include the PKA regulatory subunit,
PKA catalytic subunits, and phosphatases that act on PKA
These results appear to contradict an earlier report showing
that mutation of GPB1 and GPB2 causes changes in the intra-
cellular concentration of cAMP, which would suggest that
Gpb1p and Gpb2p modulate the activity of enzymes involved
in cAMP metabolism (14). In the previous report, it was shown
that wild-type cells and cells containing either a gpb1? or
gpb2? mutation display a twofold increase in cAMP levels
upon readdition of glucose to starved cells. However, whereas
in wild-type cells the concentration of cAMP returns to its
basal level after one minute, in mutant cells it remains at the
elevated level for several minutes. In contrast, cells containing
double gpb1? gpb2? mutations do not display a twofold in-
crease in cAMP levels upon readdition of glucose to starved
cells. Therefore, deletion of either GPB1 or GPB2 results in
slightly higher cAMP levels, and deletion of both genes results
in slightly lower cAMP levels. These observations are some-
what unexpected given that the defects conferred by individual
gpb1? and gpb2? mutations are similar to, though less severe
than, the defects conferred by double gpb1? gpb2? mutations
for all other phenotypes tested, including pseudohyphal
growth, invasive growth, FLO11 expression, heat shock sensi-
tivity, sporulation efficiency, and glycogen accumulation (1,
14). Moreover, all of the observed phenotypes of gpb1? gpb2?
cells are consistent with an increase in PKA activity, rather
than a decrease. Given that the glucose-induced spike in
cAMP levels is extremely sensitive to the nutritional status of
the cells (5), it is possible that the reported differences between
wild-type, gpb1?, gpb2?, and gpb1? gpb2? cells were caused by
differences in the nutritional state of the cells in the culture
sample used for the assay. Alternatively, the effects of GPB1
and GPB2 mutant alleles on cAMP levels could be due to a
feedback mechanism that regulates the concentration of
cAMP in response to PKA activity (25). In any case, the finding
that loss of Gpb1p and Gpb2p causes significant effects in
cyr1? pde2? and pde1? strains indicates that the kelch repeat
proteins can function independently of cAMP metabolism.
The G protein ?-subunit Gpa2p has been thought to medi-
ate signaling through direct activation of adenylyl cyclase, like
mammalian G?s, based on the following observations. First,
the glucose-induced spike in cAMP concentration is increased
in cells that overexpress GPA2, and it is eliminated in cells that
contain a gpa2? mutation when the cells are grown under
certain nutritional conditions (5, 22, 24). Second, the addition
of cAMP compensates for the defect in pseudohyphal growth
conferred by a gpa2? mutation (17, 21). However, here we
show that the Gpa2p-interacting proteins Gpb1p and Gpb2p
affect signaling through the cAMP/PKA pathway by a process
that is independent of cAMP metabolism. These observations
can be reconciled if Gpa2p and the kelch repeat proteins
impinge on the cAMP/PKA pathway at different points. Such a
situation might be expected based on the complex relationship
between the functions of Gpb1p, Gpb2p, and Gpa2p. For ex-
ample, although it has been proposed that Gpb1p and Gpb2p
bind to Gpa2p in a manner that mimics G protein ?-subunits
(14), deletion of GPB1 and GPB2 in either wild-type cells or in
gpa2? cells causes a substantial increase in PKA-dependent
phenotypes (1, 14). These results suggest that Gpb1p and
Gpb2p inhibit PKA signaling in both the presence and the
absence of Gpa2p. Therefore, one possibility is that the kelch
repeat proteins act as effectors of the G protein. However,
deletion of GPA2 in gpb1? gpb2? cells causes a two- to three-
fold decrease in FLO11 RNA abundance compared to that
seen in gpb1? gpb2? cells. Similarly, gpa2? gpb1? gpb2? cells
display a decreased level of pseudohyphal growth compared to
gpb1? gpb2? cells. Therefore, part of the signal present in
gpb1? gpb2? cells is dependent on Gpa2p. One possible model
to explain these results is that Gpb1p and Gpb2p negatively
regulate both Gpa2p and another component of the PKA
signaling pathway that is downstream of adenylyl cyclase. De-
letion of GPB1 and GPB2 activates both Gpa2p and the other
target, resulting in very high signal generation. In a gpb1?
gpb2? mutant background, deletion of GPA2 eliminates the
part of the signal that originates with Gpa2p but leaves intact
the part of the signal resulting from the other target of Gpb1p
and Gpb2p. Direct activation of adenylyl cyclase by Gpa2p


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Lingering fear over public pot smoking threatens to derail the District's marijuana decriminalization bill by keeping intact policies that allow police to stop and search people when they smell the drug, decriminalization advocates say.
Key portions of legislation to decriminalize marijuana use in the District are expected to come under fire Tuesday, when the full D.C. Council is set to consider the measure for the first time.
Lawmakers have floated the possibility of amendments to the legislation, which replaces the criminal penalties in place for marijuana possession or use with civil fines. But some council members want to amend the bill to preserve the criminal sanctions for smoking marijuana in public.
Such an amendment would "gut" the effectiveness of the proposal, said a spokesman for the bill's chief sponsor, D.C. Council member Tommy Wells, Ward 6 Democrat.
"Tommy is not going to be on board with any amendment that would strip the bill of effectiveness in actually decriminalizing marijuana," spokesman Jack Pfeiffer said.
Other amendments in the works would preserve police officers' ability to search a person or vehicle when the smell of marijuana is detected, with the goal of preventing driving under the influence. But advocates who believe that odor alone should not be cause for police to stop and search a person say such a change would defeat the bill's purpose.
"If they make changes that remove the language of reasonable articulable suspicion, it will undercut the bill," said Seema Sadanandan, program director for the American Civil Liberties Union of the Nation's Capital. "Now you have decriminalized marijuana, but it will only be decriminalized for people who were not getting stopped and searched in the first place."
Marijuana decriminalization legislation was introduced after the publication of an ACLU report highlighting the disparate rate of arrests of blacks over whites for marijuana possession in the District. The report states that blacks are arrested eight times as often as whites for the offense, even though rates of use among both races are thought to be relatively similar.
The version of the bill up for consideration would make possession of up to an ounce of marijuana a civil offense punishable by a $25 fine and confiscation of the drug. Anyone caught smoking marijuana in public could face a $100 fine. Current law makes possession punishable by up to six months in jail and a fine of up to $1,000.
"A vote to weaken any part of this bill is a vote to perpetuate racial disparities and injustice," said Bill Piper, director of national affairs at the Drug Policy Alliance. "Black men shouldn't have to fear being searched just for walking down the street, and they shouldn't face arrest or a heavy fine for doing something that affluent whites get away with every day."
Tuesday's scheduled vote will be the first of two before adoption of the legislation.


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SpringHill Suites Miami Airport South
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Suites
- Continental Breakfast
- Shuttle bus service
- Swimming pool
- Free High-Speed Internet
- Wheelchair accessibility
Travelers also recommend:
- More recommendations
Historical Traveler Reviews SpringHill Suites Miami Airport South
Great stay
I booked this hotel based on the great reviews I read and was not disappointed. Our visit was quick as we wanted somehwere close to the airport in order to catch a morning flight (shuttle leaves every hour on the hour). As was mentioned, it is a bit out of the way, but a good value. Rooms were clean and good size, the front desk helpful, and the large breakfast buffet a definite plus.
Couldnt have been more pleased
We stayed at the AmeriSuites Miami Blue Lagoon for 2 nights before a cruise at the end of May 2004. There was plenty of well-lit parking on arrival. We found the lobby to be very clean and comfortable with plenty of room for the breakfast serving area (which is in the lobby.) Our check-in was a breeze and within minutes of walking in the door we were headed up to our room. The front desk clerk was bi-lingual but spoke carefully to us and we were able to understand her easily. Even though it was late at night there was plenty of staff seen on hand and we felt very safe. The neighborhood is a bit secluded and not right in the middle of everything, which was a plus for us. Our suite was extremely clean (and I dont use that phrase generously) and spacious. We had a "TCB" handicapped suite (although we are not handicapped, it provided even more space for accessability. There were a number of other handicapped suites so I dont think we were "hogging." It wasnt a request of ours.) Upon opening our curtains we had a very lovely view of a nice section of Blue Lagoon and even the windows were clean. We never got a sense of that musty smell you get in most hotels at all, but the room smelled very clean and fresh. Even the bed was comfortable. Breakfast was sufficient and the attendant was very consciencious and friendly.
Our only complaint was that not knowing better we had scheduled our one night in Miami after our cruise at the AmeriSuites Airport West which is actually a bit further from the airport than this hotel. It was very run-down and I DONT recommend it at all.
Would Definitely Stay Here Again!
We visit Miami a lot, and this was the best hotel we've ever stayed at in Miami! Very nice and clean. Friendly and helpful staff, and delicious breakfast buffet!
Pleasantly Surprised!
After looking through all of the reviews for hotel around the airport I didn't know what to expect. I would recommend this hotel to all. It was very clean and the rooms were large with kitchenettes and a seperate sitting and work area. The complimentary breakfast was very nice. The pool was lacking but everything else made up for it.
Great Hotel!
I stayed 2 nights at this hotel. With my AAA discount it was incredibly cheap and the breakfast is really good. A friend of mine stayed in a hotel in south beach for almost the double price and their breakfast included only a bagel and coffee. Here you get cereals, bagels, muffins, eggs, danish, waffles, coffeee, orange juice etc. It is really great for the price. There is also free coffee at the reception 24 hours. The rooms are really large and clean. The hotel employees are very attentive. The view of the lake is a nice addition. I would highly recommend this hotel.
Very good choice, would recommend it!
Ok, its a little far from the action (20 minutes to South Beach), but its a great hotel. Nice and spacious rooms, very clean, friendly staff.
Lots of parking space and near the airport. I would definitely stay there again.
Great room, great services, loved the hotel
My best stay ever is with Amerisuites Miami blue lagoon. We stayed for 4 nights at this hotel, brand new rooms, clean everything, great breakfast buffet and very friendly staff. We were given a room with lake view, it was spectacular! The hotel is very close to the airport and it offers great rates too! I would strongly recommend this hotel to anyone travels to Miami!
Great Place to Stay!!
I will start off recommending Amerisuites (Blue Lagoon) to any budget minded, but demanding traveller. After exhaustively comparing the hotel deals I got from Hotels, I chose AmeriSuites because it satified my original criteria: good price ($65 a night!), free parking, pool and breakfast. The breakfast, which went from 7:30 to 9:30 (Sat & Sun: from 8:30 to 11:30) was more than satisfactory for my taste: apple and orange juice, waffles, toast, breakfast cereal, flakes and fruit, and others. The rooms were new, large and very clean, consisting of 2 double beds, a breakfast nook, "living room" with pullout couch, bathroom, area for grooming, refrigerator, sink, cabinets and microwave. I was also impressed with the view overlooking a large lake (lagoon?). (Not all rooms had this view. Some rooms overlooked the airport. I would suggest someone booking a room to request the view of the lake). I was equally impressed with the other amenities, such as the 24-hour security officer, free transportation to/from the airport, the pool (which is HEATED and always open). A free box is available at the front desk for valuables, a laundry room and fitness center. Also available was a special internet center. At the back of the hotel, there is a section, where guests can relax and take in the view of the lake enjoying a barbeque. The room service was prompt, and like all other personnel, very friendly! The distance to MIAMi (beaches) is approximately 10 minutes (take SR-839 East from the hotel). In summary, I would highly recommend this hotel to anyone.
Not for vacationers
Nice big rooms, close to the airport...but the buffet ends to early for people on vacation, and its too far from the beach.
Nice property, efficient staff
Stayed at this property 1 night. Was VERY impressed with the service of the staff and cleanliness of the rooms and entire property. Have never checked in/out of a hotel with such speed & efficiency! Airport shuttle driver went out of his way to make an extra stop for us. Did not expect a new property to have everything functioning this efficiently.
TripAdvisor Reviews SpringHill Suites Miami Airport South
Travel Blogs from Miami
... font-size: 17px; line-height: normal; -webkit-text-size-adjust: auto;">Wills was convinced he had been upside down on the Mummy ride and was disappointed when I told him it had not gone upside down. He was convinced he had survived a near death experience.
Flying Back to Toronto
... Nevertheless, two nurses came to help. We seemed to get priority landing in Toronto because of the medical emergency and had to wait for the paramedics to come on and take off the girl, who by this time claimed she was feeling much better. I whizzed through customs and then right past the baggage claim area to exit where my parents were eagerly waiting for me. Nothing like being home, well after a 1.5 hour drive more that ...
Random Zapping
... on me at all. NOPE. That's what I get for thinking the machines are actually and truly functional. I get beeped. There is nothing metal on my body at all. oh wait, my bra strap has two metal hook and eye clasps on it. Not even an underwire! No metal parts inside me either and yet I am zapped. So it has got to be random! Of course I now get a thorough pat down and that's never enjoyable to have some unknown female run ...
80F!!! thank the gods for air con!!
A smooth flight and some shut eye on the plane--all welcomed as I am a terrible flyer and I needed/wanted a few more minutes of sleep! The most excitement seemed to be the young lady beside me- needless to say I passed over my air sickness bag! American Airlines has ...
Im in miami bitch!
... a taxi, it was only then that we realised just how drunk we actually were!! We couldnt really walk and she kept throwing up, safe to say after two attempts at getting in the taxi we couldnt carry on as jess wouldnt stop being sick! So we swiftly asked for directions and paid the cab man. And started our journey of 80 blocks home!! We didnt realise how long at the time it was going to take!
Halfway through the walk home we came across ...


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I didn't recognize her not being in a fucked up twisted pretzel position.
Taking a break on her Yoga quest…..but she has done well in losing all the pregnancy weight.
Hobbit feet
Sexy little spinner.
The woman to her right is Hilaria 30 years from now trying to warn her to leave before she is fully Baldwin'd.
I feel like I'm forgetting something. Let's see.
Nanny, check.
Dogs, check.
Nope, that's everything.
She is fuckin' HOT. Hilaria, I mean, not the other one.


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Triadelphia, West Virginia Pet Friendly Hotels & Lodging
United States » West Virginia » Triadelphia
Looking for a hotel in Triadelphia, WV that you can bring your pet to? We made sure that each one of the hotels below are pet friendly, so you can enjoy your trip with your pet by your side! Once you have found a pet friendly hotel, you can book your stay instantly from our website or call 866-212-1803 to speak with a pet travel agent.
Browse Pet Friendly Hotels In Triadelphia, West Virginia
Rates from
87 Jenkins Lane - Triadelphia, WV (Map)
Econo Lodge Inn & Suites Triadelphia - Wheeling is pet friendly. Guests are welcome to bring pets no more than 25 pounds of pet. The pet fee is $25 per pet, per night.
Overview | Pet Policy | Map
Rates from
40 Robinson Dr - Triadelphia, WV (Map)
Suburban Extended Stay Hotel Triadelphia welcomes pets at their pet friendly accommodation. Up to 2 pets are allowed. The pet weight limit is 80 pounds and the pet fee is $25-75 per pet (varies based on length of stay).
Overview | Pet Policy | Map


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Days Hotel Buffalo Airport
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Continental Breakfast
- Restaurant
- Free High-Speed Internet
- Room service
- Swimming pool
- Business Services
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- More recommendations
TripAdvisor Reviews Days Hotel Buffalo Airport Cheektowaga
Travel Blogs from Cheektowaga
They told us we would get wet
... br> Very steep walk down to the boat terminal and queue for tickets. We chose the simple ride $19.95 without audio tour which was $13 bucks more. Everyone was issued with a full length pink hooded plastic poncho. (on the American side they bot blue ones and those who walked down the American side got yellow ones) Gab and Chey were a little ahead of us and got on an earlier boat. We were among he first on our ...
Another exciting day at Niagara falls
... the White Water Walk the water races north into the Eddy Basin before opening out into the Whirlpool. The 90 degree turn in the river is evidence of a 4,500 year old geographical catastrophe where the Falls once intersected with the ancient St. David's Gorge.
The Whirlpool Rapids were created in a matter of days, possibly hours when the Falls tore out the glacial debris that filled the older gorge. Today the 90 ...
March-ean-ing on to Niagara
... checked in and headed straight for the mist. Wow, the falls are pretty big and noisy. A few snaps, then we headed to the hotel for a quick shower then dinner. Dinner was at Keg Steak House, we both ordered steak, but they were pretty small serves. It was then off for the fireworks display over the falls. Same old stuff, however little did we know the fireworks were going to continue in Room 109. The travellers resided in Room 107, which ...
The Big Day
... microphones, cables and sound gear weren't there yet - it was all coming with Mike, so they also sat around and watched the fun too. Meanwhile the girls in the party had a big room up on the 8th floor opposite ours to get dressed in, and the boys in the party used our room, which was kind of convenient as things and messages had to be passed between them from time to time, and the photographer didn't have to go far.
Mike and Lynne arrived about 10, and Mike started setting up ...
We're All Here
... the road to drop off one of Ashley's guitar pedals for repair, and lastly back to the tailor to drop Ashley off for more fittings. Mandy and I then sprinted out to the airport to drop off the car with 15 minutes to spare. The flight that Mandy's parents were arriving on was delayed by about two hours, so a convenient timing turned out to be quite a lengthy wait for Mandy, while I took ...


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I play too much fantasy football, but that doesn't necessarily mean I'm an expert when it comes to drafting, setting lineups, and scouring the waiver wire for fantasy sleepers. In all honesty, I love joining my friends' leagues, even if I've yet to come away with the elusive championship prize in nearly ten years of play.
Case in point — my family owns a coffee shop, and I started an eight-man league with our baristas last year, thinking I'd waltz to a title against the less-experienced coffee makers. By the end of the season, I finished in fifth place, missed the playoffs, and watched my twin sister dominate to finish as the league champion. Fantasy football is a cruel, but rewarding endeavor given the unpredictable nature of the "sport," and that's what makes the Vikings Territory contest so exciting!
As Brett has told you before, we're hosting a weekly contest through Draft Kings to bring Vikings fans together through easy-to-join DFS contests. For just $5, you can take part in the league and compete against a few of the VT writers, including myself, Brett, Adam, Brian, and Matt Engstrom! Setting your lineup takes no more than 10 minutes, and the top-3 teams walk away with cash prizes.


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GPU cracks 6 character password in 4 seconds
"People have worked out that the processing power of graphics cards, due to the architecture of the chips, is more powerful than a normal processor for doing certain tasks," said Neil Lathwood, IT director at UKFast.
About the author
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Booktuation: 'She is a very sweet and sensitive character who is difficult not to get along with'
From Tracy Beaker to Wuthering Heights to A Series of Unfortunate Events, the author of Salvage shares her favourite books about fostering and adoption
Bookworm Reader: 'It's a very hooking, descriptive and exciting book, I can't wait to read another book of Jacqueline Wilson's!'
The former Children's Laureate writes a love letter to Kingston Library, where she discovered Laura Ingalls Wilder and Virginia Woolf
The cover of The Butterfly Club – Jacqueline Wilson's 101st book – is revealed and recreated in glorious loom band technicolour!
SophieMaxim: 'Once you start reading, you will enjoy it and will be unable to take your eyes off the page!'
Mya C: 'It was very funny, and sad in some places'
Charlie123: 'This was Jacqueline Wilson's 100th book. It was also undoubtably one of her best, it's cleverly written with lots of description and links to real issues that happened in 1913-1914'
bunkbedbobby: 'I like this gripping tale because you never know what's around the corner for Sapphire Battersea'
Want to experience the magic of illustration in motion? Then watch these six-second videos of top children's illustrators drawing. From Axel Scheffler to Debi Gliori, watch the finest British illustrators doodling beautifully for us
Site members Ayesha and LottieLongshanks got to read Jacqueline Wilson's 100th book before it was published – and ask Dame Jacky their questions about Opal Plumstead and beyond!
Newsflash: the Reading Agency has started a poll to find out what book makes the most people the happiest. Help them find out!
Scoutingforbooks: 'What I thought was a great book, is now just another cliched Jacqueline Wilson storyline'
Scoutingforbooks: 'There is not a multi-cultural character in sight'
Back to school means back to your friends (and all the delights and strains that brings) – but how much do you know about the friendships explored in Jacqueline Wilson's books? Test your skills here!
Imogen Russell Williams: Aldi's decision to withdraw his book from sale in Australia is unfair to children, who appreciate – and need – a taste of rude reality
'Some of them look like opium dreams, which perhaps they were'
Statues of the great and good across the capital and Manchester will be able to chat to passers-by thanks to smartphone technology, writes Kit Buchan
How did Jacqueline Wilson's wonderful book Hetty Feather make it to the West End? The people who adapted the book into a play – plus Jacqueline – explain how it was done
Bookworm Reader: 'Jodie means everything to Pearl. She's her sister, her best friend, all until they start attending Melchester College'
Newsflash! A first look at Opal Plumstead, schoogirl, sweet factory worker and suffragette – and tipped to be Jacqueline Wilson's most outspoken and fiery heroine yet!
TMandy: 'My Sister Jodie is an emotional book written by an incredible author, and I would encourage anyone to read it. But be warned, you may need a box of tissues!'
Victoria – Millennium RIOT Readers: 'I give it a big 5/5 stars because it is so good!'
happyhorse911w: 'I like the way that Jacqueline Wilson made Pearl's adventure seem so real'
Life after Tracy Beaker and Hetty Feather: a locum Book Doctor has some top reading tips for nine-year-olds who love Jacqueline Wilson books so much they don't know what to read now they've finished reading them all!
My eight-year-old daughter and I couldn't wait to see this adaptation of Jacqueline Wilson's much-loved story – and it didn't disappoint, writes Emily Drabble
Jacqueline Wilson: When Novel Theatre wanted to stage my book, I felt as proprietorial as any mother. Who was going to play my Hetty?
Kat Winter: 'I read this book in a day because when I start I can't stop reading'
Shirin123 ''Altogether I loved the book and was drawn in by the very first sentence!"
- Children and teenagers
- Children's books: 8-12 years
- Friendship (children and teens)
- Teen books
- Historical fiction (children and teens)
- Children's books: 7 and under
- School
- Theatre
- Animals
- Circus
- Children's theatre
- Edinburgh international book festival
- London
- Anne Fine
- Funny books (children and teens)
- Fostering
- Art
- Roald Dahl
- Jeremy Paxman
- Mobile phones


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View by: Most Popular | Alphabetical A to Z | Map
Giza Pyramids
The sole survivor of the Seven Wonders of the World, the Pyramids of Giza still live up to more than 4,000 years of hype. Their extraordinary shape, geometry ... Read more
Hanging Church (El Muallaqa, Sitt Mariam, St Mary)
Dedicated to the Virgin Mary, the Hanging Church, which is still in use, is called the Hanging or Suspended Church as it is built on top of the Water Gate of ... Read more
Khan al-Khalili
Jaundiced travelers often dismiss the Khan al-Khalili as a tourist trap; there's no ignoring the fact that it's a favored stop of tour buses and has all the ... Read more
Lake Nasser
Named after the Egyptian President who started the process, Lake Nasser is a result of the construction of the Aswan High Dam which effectively modernised ... Read more
Luxor Museum
While the size of its collection can't rival the treasures of Cairo, Luxor Museum is renowned as one of the thoughtfully assembled displays of antiquities ... Read more
Luxor Safaga Cruise Port
Located on Egypt's Red Sea coast, the port of Safaga has a small but lively tourism industry, primarily centered on scuba diving and surfing. For most cruise ... Read more
Luxor Temple
The enormous Luxor Temple was one of the great constructions of the New Kingdom (dating from the 14th century BC) dedicated to the god Amun. It was known as ... Read more
Mahmaya Island
This small island off the coast of Hurghada offers access to some of the clearest, bluest waters of the Red Sea away from the busy nearby shores. Accessed by ... Read more
Makadi Water World
This beachfront water park in Hurgada is a favorite for many families visiting the area. With 46 different water slides, a wave pool, water cannons, a wave ... Read more
Marine Biology Museum
Hurghada's Marine Biology Museum reveals insights into the aquatic wonderland lying offshore. Whether you're a keen snorkeler or want to learn more about the ... Read more
Medinet Habu (Temple of Ramses III)
Where the fertile Nile floodplain meets the desert lies the Mortuary Temple of Ramses III, known locally by its Arabic name Medinet Habu. The whole compound ... Read more
The city of Memphis was the capital of ancient Egypt. It was the King's residence and the political and administrative center until around 2,200 BC. It had ... Read more
Mons Porphyrites
The short trip to Mons Porphyrites is a popular excursion from Hurghada. Egypt's rare porphyry was highly valued by the ancient Romans, and these former ... Read more
Mount Sinai
For many visitors to St. Catherine's Monastery at the foot of Mount Sinai, the trek to the mountain's 2,285 meter (7,495 foot) summit is a highlight of ... Read more
Na'ama Bay
The focus of the Sharm el Sheikh resort action is Na'ama Bay, a collection of glittering seaside resorts fronting the water. Stroll the beachfront ... Read more


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Notes from the Manager
Related Strips: #556: The Third Miracle, Part Two; #565: What Price Victory?; #566: Do the Right Thing, Part One; #568: In the Gay Way
Some of you are going to say this little breakthrough of Jason's is ridiculous (and it's supposed to be… obviously), but at the same time, this realization that opinions of movies are (at least to some extent) a subjective thing is something a lot of you guys haven't managed to fully grasp, either. So… :P
Does this epiphany mean we're going to see a kinder, gentler, less-snobby Jason in the future…?! I wouldn't count on it, but stay tuned!!!
How much Multiplex can you handle?!
Multiplex is made possible by Andrew Hathaway at Can't Stop the Movies and readers like you via Patreon, who contribute over $600 per month to keep Multiplex updating and ad-free.
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Become a Multiplex Patron today!
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Deleted Scenes Blog
Bonus comics, drawings,
movie trailers and more
Major NASA Announcement
Tuesday, September 29, 2015
I know I don't usually post stuff like this here, but I was so amazed by this major NASA announcement yesterday. Check it out!
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SEC Awards Whistleblower Maximum Possible Share of Settlement in Dodd-Frank Retaliation Case
On April 28, 2015, the Securities and Exchange Commission announced that it was awarding a whistleblower 30 percent of funds recovered in settlement of the Commission's first retaliation charges brought under the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank).
The whistleblower's share will be more than $600,000. In deciding to award the maximum 30 percent, the SEC's Claims Review Staff weighed heavily the "substantial evidence that the whistleblower suffered unique hardships as a result of reporting."
In the Matter of Paradigm Capital Management, Inc. and Candace King Weir, File No. 3-15930 (June 16, 2014), the SEC charged the hedge fund investment adviser with retaliating against the whistleblower for reporting what the whistleblower believed to be misconduct to the SEC. The SEC found that Paradigm removed the whistleblower from the whistleblower's then-current position, changed the whistleblower's job function, and removed the whistleblower's supervisory responsibilities, among other retaliatory acts.


text: The Leonard Lopate Show Edit this Published in The Leonard Lopate Show The West in Iraq Listen 33 min Queue Download this audio Want to embed this player? Use the code below. More info. <iframe frameborder="0" scrolling="no" height="130" width="100%" src="https://www.wnyc.org/widgets/ondemand_player/wnyc/#file=/audio/json/53150/&share=1"></iframe> Share on Facebook Share on Twitter Email a Friend Barry Lando, a former investigative producer for "60 Minutes," exposes the ways in which Western interests helped devastate Iraq during the years of Saddam Hussein's rule. Web of Deceit is available for purchase at amazon.com


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You can buy this painting from this link (afremov.com) using a 10% discount coupon - deviantart10off
i like your style, watch for the shapes give them more mistery, great job. like your work
beautiful!!!! I love your paintings!!
Love the brush stroke.
Is that acrylic painting?
So beautiful....
So beautiful....
Wow, great job! You have an eye for color.
-pulls on hair- ugh!!! i wish i could paint like this!!!! argh. amazing!
Really fascinating style man!
it looks so creamy and juicy. I have to say I'm jealous
I just can confirm that. Love the colours!
The colors you used are absolutely beautiful! Great work as always.


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Vote for your favourite goal of the 12 listed below
Due to a technical issue we've had to take this poll down and create a new one. Please vote again on the new poll.
Last week we revealed our favourite goals from Europe in the past season and asked you to flag up yours. We've trawled through the list of stunning strikes, delicate chips and audacious displays of goalscoring from the continent and compiled the definitive list. Click on the links below to view the goal then cast your vote.
1) Yoann Gourcuff (BORDEAUX v Paris St Germain, Ligue 1)
2) Simon Cox (SWINDON TOWN v Walsall, League One)
3) Grafite (WOLFSBURG v Bayern Munich, Bundesliga)
4) Fernando Torres (LIVERPOOL v Blackburn, Premier League)
5) Achille Emana (BETIS v Sporting, La Liga)
6) Remi Mareval (NANTES v Marseille, Ligue 1)
7) Cristiano Ronaldo (Porto v MAN UTD, Champions League)
8) Moussa Dembélé (Willem II v AZ ALKMAAR, Eredivisie)
9) Eduardo (ARSENAL v Burnley, FA Cup)
10) Zlatan Ibrahimovic (INTER v Bologna, Serie A)
11) Geovanni (Arsenal v HULL, Premier League)
12) Giuseppe Mascara (Palermo v CATANIA, Serie A)
Would you have picked a different goal? Or why is the strike you have chosen a worthy winner? Have your say in the comments section below.


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Houses to let in Hertford Road, Enfield EN3 - 2 bedrooms
Keywords and filters like garden and parking live here
Your search area is too large to further filter your results. Please edit your search location if you would like to filter your results.
Filter your 1 result Clear filters
Property type
Letting type
£1,300 pcm (£300 pw)
Connop Road, Enfield EN3End terrace house to rent - 2 bedrooms
Lords Property is pleased to offer this immaculate 2 bedroom newly built house to let in Enfield, EN3 More details
Note: Distances are straight line measurements
- Turkey Street (0.5 miles)
- Enfield Lock (0.7 miles)
Marketed by Lords Property, EN3. Call 020 3641 4212


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"Amen" (feat. Drake)
Meek Mill's hit of the summer, featuring Drake, makes an encore appearance after debuting on Dreamchasers 2. In between the album's theatrical sturm and drang, it's a welcome light and happy moment, even if we've heard it a million times before.
(Photo: Courtesy of MMG)
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A woman returns to the Philippines after working abroad to find that her family has fallen apart in her absence.
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