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On Tuesday, President Barack Obama, Vice President Joe Biden and U.S. Transportation Secretary Ray LaHood marked the release of $26.6 billion from the American Recovery and Reinvestment Act (ARRA) to states and local transportation authorities to repair and build highways, roads and bridges.
The release of the funds came eight days earlier than required by law and took place before more than 500 headquarter employees at the U.S. Department of Transportation (U.S. DOT).
"President Obama is keeping his promise to the American people and he is doing it ahead of schedule," said Secretary LaHood. "This is a long-overdue investment in our transportation infrastructure and in jobs for Americans."
State highway departments have already identified more than 100 other transportation projects across the country where construction can start within the month, totaling more than $750 million. That number is expected to grow substantially as more states submit certifications and begin to receive project approvals.
Each proposed project must be approved by the department's Federal Highway Administration. Each state's governor must certify that their proposed projects meet certain conditions and that the state will use ARRA funds in addition to, and not to replace, state funding of transportation projects. The U.S. DOT will monitor state compliance and track job creation. The projects will be posted on the Web for the public to see, with information on projects accessible at www.recovery.gov.
President Obama's visit to U.S. DOT headquarters marks the first by a sitting U.S. President with his Vice President. At the ceremony the President unveiled new logos for government-wide projects completed under the ARRA and the Transportation Investments Generating Economic Recovery logo for transportation projects completed under the ARRA.
To enhance understanding of the Act, the new circular offers a user-friendly, one-stop resource on its requirements.
The course is instructed by Halsey King, a well renowned speaker and writer on maintenance and technology issues, that includes repair, inspection and management of vehicle fleets.
The about-face came just six days after an internal audit of the city's public transportation system was released to the council. In it is a recommendation to raise paratransit rates to $2.50 a ride from the $2 fee riders have paid since 1996.
Since the ADA's signing in 1990, AC Transit and BART have worked to ensure disabled residents are able to enjoy the many benefits of public transportation. The two agencies joined together in 1994 to form the East Bay Paratransit Consortium.
A paratransit fare increase may be proposed to fill the gap; however, Pace Executive Director T.J. Ross acknowledged it would be very difficult for those riders to absorb.
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College football locals: Sept. 29, 2012
NAPLES - How local players fared in Week 5 of college football:
Dallas Crawford (South Fort Myers), Miami, x-freshman, running back, No. 25 — Crawford did not play in the Hurricane's 44-37 win over North Carolina State.
Makinton Dorleant (Lely), Maryland, x-freshman, defensive back, No. 23 — The Terrapins were off.
Nate Dortch (South Fort Myers), Miami, freshman, defensive back, No. 35 – Dortch did not play in the Hurricane's 44-37 win over North Carolina State.
Sammy Watkins (South Fort Myers), Clemson, sophomore, wide receiver, No. 2 — Watkins did not play because of an abdominal virus in the Tigers' 45-31 win over Boston College.
Brodrick Jenkins (South Fort Myers), West Virginia, x-junior, defensive back, No. 23 — Jenkins had two solo tackles in the Mountaineers' 70-63 win over Baylor.
Marvin Kloss (Barron Collier), South Florida, x-sophomore, kicker, No. 27 — Kloss averaged 64.2 yards on four kickoffs in the Bulls' 30-17 loss to Florida State.
J.D. Woods (Golden Gate), West Virginia, x-senior, wide receiver, No. 81 — Woods had 13 catches for 114 yards, rushed for 16 yards and had one touchdown in the Mountaineers' 70-63 win over Baylor.
Torrey Campbell (Barron Collier), Iowa, x-freshman, defensive back, No. 22 — Campbell did not play in the Hawkeyes' 31-13 win over Minnesota.
Jeff Heuerman (Barron Collier), Ohio State, sophomore, tight end, No. 86 — Heuerman played, but did not register any stats in the Buckeyes' 17-16 win over Michigan State.
Carlos Hyde (Naples), Ohio State, junior, running back, No. 34 — Hyde had 11 rushes for 49 yards, one pass reception for 6 yards, and one solo tackle in the Buckeyes' 17-16 win over Michigan State.
Jack Swanson (Naples), Iowa , x-senior, defensive back, No. 40 — Swanson had one assisted tackle in the Hawkeyes' 31-13 win over Minnesota.
Patrick Bastien (Immokalee), UAB, x-junior, linebacker, No. 47 — Bastien had six tackles, five solo and one assisted, with one tackle for a loss of 4 yards in the Blazers' 49-42 loss to Tulsa.
Joseph Puopolo (Barron Collier), UCF, x-freshman, running back, No. 44 – Puopolo did not play in the Knights' 21-16 loss to Missouri.
Anthony Spano (Gulf Coast), Marshall, x-freshman, offensive lineman, No. 68 — Spano did not play in the Thundering Herd's 51-41 loss to Purdue.
Stanley Sylverain (Golden Gate), UCF, freshman, linebacker, No. 42 – Sylverain did not play in the Knights' 21-16 loss to Missouri.
Jhony Faustin (Lely), Northern Illinois, junior, defensive back, No. 28 — Faustin had three solo tackles in the Huskies' 55-24 win over Central Michigan.
Shaquille Hall (Palmetto Ridge), Bowling Green, x-freshman, defensive end, No. 56 — Hall played but did not register any stats in the Falcons' 48-8 win over Rhode Island.
Tronic Williams (South Fort Myers), Western Michigan, x-junior, cornerback,No. 9 — Williams played but didn't register any stats in the Broncos' 37-17 loss to Toledo.
Nick Alajajian (Naples), Florida, senior, defensive lineman, No. 58 — The Gators were off. Alajajian is out for the season with a leg injury.
Carl Elie (Immokalee), Louisiana-Monroe, x-senior, running back, No. 20 — Elie did not play in the Warhawks' 63-10 win over Tulane.
Mohammad Farah (Estero), Florida Atlantic, freshman, offensive lineman, No. 76 — Farah did not play in the Owls' 20-14 loss to North Texas.
Jack Griffin (St. John Neumann), Florida International, senior, kicker, No. 38 — Griffin kicked off five times with two touchbacks in the Panthers' 48-20 loss to Louisiana-Lafayette. Griffin was also 2-for-2 on extra-point attempts.
Michael Kee (Naples), Middle Tennessee State, x-freshman, punter — Kee did not play in the Blue Raiders' 49-28 win over Georgia Tech.
Mitch MacClugage (Barron Collier), Florida International, x-senior, long snapper, No. 65 — MacClugage played but didn't register any stats in the Panthers' 48-20 loss to Louisiana-Lafayette.
Juno Prudhomm (Naples), Middle Tennessee State, senior, safety, No. 42 — Prudhomm played but did not register any stats in the Blue Raiders' 49-28 win over Georgia Tech.
Kenneth Mouton (Golden Gate), Navy, quarterback – Mouton did not play in the Midshipmen's 12-0 loss to San Jose State.
* * *
Justin Black (Barron Collier), Northern Iowa, freshman, quarterback, No. 10 — Black did not play in the Panthers' 33-21 loss to North Dakota State.
Fedney Delphonse (Golden Gate), Eastern Illinois, x-freshman, defensive lineman, No. 98 — Delphonse had three tackles, two solo and one assisted, and a tackle for a loss of 1 yard in the Panther's 65-15 win over Austin Peay.
Evan Goldszak (Barron Collier), Colgate, senior, punter, No. 24 — Goldszak average 31 yard on three punts in the Raiders' 47-24 win over Yale.
Emmanuel Edouard (Lely), Colgate, senior, running back, No. 46 — Edouard played but did not register any stats in the Raiders' 47-24 win over Yale.
Darion Hall (Lely), Tennessee State, x-sophomore, running back — Hall played but did not register any stats in the Tigers' 40-13 win over Arkansas-Pine Bluff.
Peterson Janvier (Golden Gate), Morgan State, freshman, strong safety, No. 29 – Janvier did not play in the Bears' 21-18 win over North Carolina A&T.
Sadath Jean-Pierre (Immokalee), Citadel, x-junior, defensive back, No. 1 — Jean-Pierre had 11 tackles, seven solo and four assisted, in the Bulldogs' 28-10 loss to Chattanooga.
Andrew Jones (Naples), William & Mary, x-freshman, offensive lineman, No. 77 — The Tribe played against Georgia State on Saturday night.
Thomas Martin (Naples), Morgan State, freshman, wide receiver, No. 10 — Martin did not play in the Bears' 21-18 win over North Carolina A&T.
Layne McCombs (Naples), Jacksonville, x-senior, linebacker, No. 45 – McCombs had six tackles, one solo and five assisted, and one tackle for a loss in the Dolphins' 26-14 win over Marist College.
Emmanuel Orange (Naples), Georgia Southern, x-freshman, cornerback, No. 25 — The Eagles played Samford on Saturday night.
Greg Pratt (Naples), Liberty, x-junior, wide receiver, No. 15 — The Flames were off.
Luke Smith (South Fort Myers), Jacksonville State, freshman, tight end, No. 80 – Smith had one reception for 7 yards in the Gamecocks' 31-16 win over Southeast Missouri.
NOTE: Information is taken from the college or university athletics website. Email corrections or additions to firstname.lastname@example.com
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Page 1
Human herpesvirus 1 protein US3 induces an
inhibition of mitochondrial electron transport
Mohammad Derakhshan, Margaret M. Willcocks, Michael A. Salako,3
George E. N. Kass and Michael J. Carter
Michael J. Carter
School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK
Received 14 February 2006
Accepted 30 March 2006
Previous studies have identified virus proteins that traffic to mitochondria and may affect
mitochondrial function. Here, it is reported that Human herpesvirus 1 (HHV-1, herpes simplex virus
1) and influenza virus reduced mitochondrial respiration, whilst Measles virus, cytomegalovirus,
coxsackievirus B4 and Feline calicivirus did not. The inhibition of total cellular respiration was
caused by a block in the mitochondrial electron-transport chain. This effect occurred during
b-phase protein synthesis and the inhibition of mitochondrial respiration could be reproduced by
ectopicexpressionofthe b-phaseproteinUS3.AnHHV-1mutantlackingthisproteinfailedtoinhibit
oxygen consumption in infected cells relative to controls. It was concluded that US3 was mediating
the suppression of mitochondrial respiration following HHV-1 infection. The integrity of the
electron-transport chain in HHV-1-infected cells was analysed further and the site of the block in
electron transport was located between complexes II and III, a site previously shown to be affected
by Poliovirus.
Many viruses affect mitochondria: morphological altera-
tions are induced by human immunodeficiency virus
(Radovanovic et al., 1999), human T-cell leukemia virus
type 1 (D'Agostino et al., 2002) and Rubella virus (Lee et al.,
1999), and changes in location are observed in infection by
Human herpesvirus 1 (HHV-1, herpes simplex virus 1) and
Hepatitis B virus (Murata et al., 2000; Takada et al., 1999).
Virus proteins may traffic to mitochondria (Henkler et al.,
2001) and affect the function of mitochondrial components
to mitochondrial inner membranes, triggering apoptosis
(Gibbs et al., 2003; Yamada et al., 2004). However, relatively
little is known about how such processes might affect the
basic mitochondrial function of energy generation. Norkin
(1977) demonstrated that total cellular respiration was
reduced by simian virus 40 infection of CV-1 cells, although
levels of ATP were maintained. Poliovirus induces a rapid
inhibition of host-cell respiration and blocks electron tran-
sport between complexes II and III (Koundouris et al.,
In contrast, ATP levels decline during HHV-1 infection,
which has been attributed to mitochondrial dysfunction
(Murata et al., 2000). We hypothesized that such effects
might be common in virus infection and have tested
different viruses. We found that HHV-1 and influenza
virus both inhibited the mitochondrial respiratory chain.
Further investigation of the HHV-1-induced effect showed
that the virus targeted a site between complexes II and III
and that this was mediated by protein US3.
Cellular respiration was measured by using a Clark-type
oxygen electrode (OE) (Hansatech Instruments). Cells were
infected with 10 p.f.u. per cell by using the following virus–
cell combinations: HHV-1 (strain HFEM, kindly provided
by Professor A. C. Minson, Cambridge, UK), Measles virus
(Edmonston strain, kindly provided by Professor V. ter
Meulen, Wu ¨rzburg, Germany) and coxsackievirus (strain
B3) were grown in HeLa cells; cytomegalovirus (strain Ad
169, kindly provided by Professor A. C. Minson) was culti-
in CRFK cells and influenza virus (strain A/Puerto Rico/8/
34, kindly provided by Dr P. Digard, Cambridge, UK) was
grown in MDCK cells. Both mock- and virus-infected cells
were recovered by scraping and their viability was deter-
mined by dye exclusion. Cells (36106) were washed with
Dulbecco's PBS and resuspended in 0?4 ml Dulbecco's PBS
for transfer to the OE for measurement of oxygen con-
sumption. Oxygen consumption was attributed entirely
to mitochondria, as inhibition of mitochondrial electron
transportbyantimycinA(3 mg ml21)abolishedmeasurable
uptake completely.
Only HHV-1 and influenza virus (Fig. 1a and b) suppressed
cellular respiration. Feline calicivirus, Measles virus, cyto-
megalovirus and coxsackievirus B4 had no detectable effect
(data not shown). HHV-1 was the more potent, reducing
oxygen consumption rate by 31% at 6 h post-infection
(p.i.), 54% at 12 h p.i. and 60% at 18 h p.i. (Fig. 1a).
Influenza virus reduced consumption by 30% at 6 h p.i.
and by 45% at 12 h p.i. (Fig. 1b). The viabilities of infected
and uninfected cells were not significantly different at these
3Present address: Cancer Research UK, London EC1M 6BQ, UK.
0008-1949 G 2006 SGMPrinted in Great Britain 2155
Journal of General Virology (2006), 87, 2155–2159
DOI 10.1099/vir.0.81949-0
Page 2
times. Inhibition of cell respiration occurred well before the
onset of cytopathic effect.
Influenza virus protein PB1-F2 is known to collapse
mitochondrial membrane potential (Gibbs et al., 2003),
which may be responsible for the effect observed. However,
we analysed the integrity of the electron-transport chain in
infected cells to locate the site of any blockage. HHV-1-
infected (12 h p.i.) or mock-infected HeLa cells were
harvestedasabove,washed twice withmitochondrial respir-
ation buffer (210 mM mannitol, 70 mM sucrose, 1 mM
EGTA, 5 mM HEPES, pH 7?1), resuspended in 0?4 ml
mitochondrial respiration buffer and transferred into the
OE. Digitonin (0?375 mg ml21) was added to permeabilize
the plasma membranes. Respiration was monitored fol-
lowing the addition of substrate electron donors feeding
electrons into known points in the electron-transport chain.
Whenappropriate, electron transportthroughspecificcom-
plexes was subsequently blocked by using specific inhibitors
to confirm observations. Substrates/inhibitors used were:
pyruvate(5 mM)plusmalate(5 mM),rotenone(3 mg ml21),
succinate(5 mM),antimycinA(3 mg ml21),tetramethyl-p-
phenylenediamine (TMPD) (0?5 mM) plus ascorbate
(1 mM), and sodium azide (5 mM). The results are pre-
sented in Fig. 2. Complex I was assessed by the addition of
pyruvateplusmalate(P/M)todonateelectrons tocomplexI
and subsequently by the addition of rotenone to prevent
their onward transmission to complex II. We detected no
increase in oxygen consumption following pyruvate and
Fig. 1. Effect of virus infection on total cell respiration. In each
case, cells were infected or mock-infected with virus and
harvested at the indicated times p.i. Total cell respiration was
determined in infected and control cells by using an OE. (a)
HHV-1 infection of HeLa cells. $, Mock-infected cells; #,
virus-infected cells. (b) Influenza virus infection of MDCK cells.
Filled columns, mock-infected cells; shaded columns, virus-
infected cells. Asterisks indicate significant divergence from the
control values: *, P<0?05; **, P<0?01; ***, P<0?001.
Fig. 2. Analysis of mitochondrial respiratory-chain integrity.
Mitochondrial respiration was determined in mock-infected (a)
and HHV-1-infected (b) cells with permeabilized plasma mem-
brane to permit the addition of electron donors to selected
parts of the respiratory chain. Respiration was monitored in real
time and the resulting traces are presented, with the times of
addition of the various compounds indicated by arrows above
the line. Downward deflection indicates increased use of oxygen
following electron-donor addition. Any such effects were con-
firmed by addition of the appropriate inhibitor to block induced
flow and before the next complex in the chain was analysed.
Compound additions were as follows: addition of digitonin (Dig)
to permeabilize the cells; pyruvate/malate (P/M) (for complex I);
succinate (Suc) (for complex II) and TMPD/ascorbate (T/A)
(electron donation to cytochrome c). The inhibitors used were
rotenone (Rot) (blocks complex I), antimycin A (AA) (blocks
between complexes II and III) and sodium azide (Az) (blocks
complex IV). Note the differential effect of succinate addition
between infected and mock-infected cells. Each trace is repre-
sentative of at least three independent experiments.
2156 Journal of General Virology 87
M. Derakhshan and others
Page 3
malate addition, even in the controls, probably because
endogenous substrates were present in excess, saturating
flow through this complex. Assessment of complex II by the
addition of succinate clearly stimulated oxygen consump-
(Fig. 2a), demonstrating that electron transfer from com-
plex II to oxygen via complexes III and IV was responsible.
This stimulation was absent in HHV-1-infected cells
(Fig. 2b) and antimycin A had no inhibitory effect. This
confirmed that electrons donated by the succinate were not
able to flow on to complex III and located an infection-
specific respiratory-chain block between complexes II and
III. We then investigated electron flow post-cytochrome c
to complex IV by using TMPD/ascorbate. Following the
showed an increased rate of oxygen consumption (Fig. 2a,
b) and thus electrons were able to flow from cytochrome c
to complex IV. As expected, in all cases, sodium azide
blocked respiration completely. These data confirmed that
complex IV was functional in both infected and mock-
infected cells. We concluded that the most likely site of the
virus-induced electron-transport block was between com-
plexes II and III, although an additional block at complex I
could not be excluded.
One possible explanation for this effect could be that viruses
synthesize proteins that interact with mitochondria and
modulate the organelle's activity: the pseudorabies virus b-
protein US3 is known to traffic to the mitochondria (Calton
et al., 2004) and work by Reynolds et al. (2002) has
demonstrated that the equivalent protein made by HHV-1
concentrates in the perinuclear area, the same site to which
mitochondria are known to migrate in the infected cell
(Murata et al., 2000). Suspicion thus fell on the HHV-1 US3
proteinand this was also consistent with the time of onset of
the effect, which in our hands occurred during the b phase,
well before the onset of synthesis of the gamma-phase
marker protein glycoprotein D. Consequently, we sought
to examine the role of US3 directly by specific expression of
this protein inside uninfected cells. In order to avoid effects
caused by any carrier or polymerase-expressing viruses, we
decided against vaccinia T7-based or baculovirus expres-
sion systems and selected instead direct transfection using
mRNA produced in vitro. For this purpose, HeLa cells were
grown in 75 cm2tissue-culture flasks, infected with HHV-1
at an m.o.i. of 10 p.f.u. per cell and harvested when a
cytopathic effect was visible. Total RNA was extracted by
using RNAzol B (Biotecx Laboratories) and the US3 coding
RNA. The following primers were designed to introduce
unique restriction sites at each end (HindIII and XbaI) for
ease of subsequent cloning (underlined): reverse (antisense)
primer, 39-GTCAGTCTAGATCATTTCTGTTGAAACAG-
CGG-59, and forward (sense) primer, 59-CGAAGCTTCGA-
The US3 PCR-amplification products were cloned into
pGEM-T Easy vector (Promega), verified by sequencing
and subsequently excised from the vector by using HindIII
and SpeI for forced-orientation ligation into the pSP64
Poly(A) vector (Promega) from which mRNA transcripts
could be prepared in vitro using an Ambion mMESSAGE
mMACHINE SP6 transcription kit. The gene for luciferase
was similarly cloned into pSP64 Poly(A) for use as a control
to determine both transfection efficiency and the optimum
scripts were verified as active by translation of the mRNA to
be used in transfection in vitro. In both cases, products of
the expected size were derived (data not shown). HeLa cells
were transfected with 2 mg mRNA prepared in vitro by using
Lipofectin (Invitrogen). Expression of transfected mRNA in
detected by luminometry. Peak expression occurred after
6 h. Transformation efficiency was determined as 80% by
immunostaining of transfected cells with anti-luciferase
antibody (Promega). In order to determine the effect of the
US3 gene product on mitochondrial respiration, we used
both mRNAs in separate transfections and compared these
with a 'no RNA' control. At 6 h post-transfection, we
measured total HeLa cell respiration in all samples in the
OE. Transfection with US3 mRNA affected mitochondrial
respiration profoundly, resulting in a decrease in total cell
luciferase mRNA, which were indistinguishable from the
mock-transfected cells (Fig. 3a).
This analysis indicated clearly that protein US3 alone was
capable of inducing a reduction in respiration; it did not,
however, demonstrate whether US3 is the only virus protein
contributing to this effect. In order to investigate this
possibility, wemade useofawell-characterized US3deletion
mutant (and its wild-type progenitor), kindly provided by
Professor B. Roizman (University of Chicago, IL, USA) and
reported previously (Purves et al., 1987). This mutant
expresses only the first 68 residues of US3, with aa 69–357
deleted, including all motifs associated with protein kinase
function; residues beyond the deletion are frame-shifted.
This mutant (termed R7041 in the original report) lacks
detectable protein kinase activity, a phenotype that is
reversed when the deletion is reverted (Purves et al., 1987).
R7041 was constructed in HHV-1 (strain F) virus and it
was necessary first to establish that this strain also exhibited
the inhibition of mitochondrial respiration that we had
cytopathic effect in both wild-type and mutant virus was
indistinguishable from that observed using strain HFEM,
and HHV-1 strain F wild-type virus clearly induced an
identical effect on respiration, establishing a 49% reduction
by 12 h p.i. In comparison, the US3 deletion mutant failed
to inhibit oxygen consumption and respiration was indis-
tinguishable from that of mock-infected cells (Fig. 3b). As
the function of US3 had already been established, it was not
necessary to consider the revertant virus in this experiment.
US3 clearly has a role in pre- and post-mitochondrial
modulation of apoptosis in HHV-1-infected cells (Asano
http://vir.sgmjournals.org 2157
Herpesvirus mitochondrial respiration inhibition
Page 4
et al., 1999, 2000; Benetti & Roizman, 2004; Cartier et al.,
2003; Geenenet al.,2005;Goshima et al.,1998; Jerome etal.,
1999; Leopardi et al., 1997; Munger & Roizman, 2001; Ogg
et al., 2004). Here, we have also shown an effect on the
mitochondria themselves. It is becoming increasingly
apparent that many viruses interact with mitochondria in
the infected cell, possibly in an attempt to modulate the
may have an effect on mitochondrial function, whether or
not this is their primary role. Mitochondrial dysfunction
may be relatively insignificant in acute infection unless it
reduces energy supply sufficiently to impair virus replica-
tion. However, effects such as these may gain in impor-
tance in the context of longer-term persistent or chronic
infections, where the cell's energy balance may be subtly
altered, perhaps affecting cell function. For example,
following infection with Hepatitis C virus, oxidative injury
occursthroughthegenerationofreactiveoxygen speciesasa
direct result of mitochondrial dysfunction (Okuda et al.,
2002). Hepatitis C virus core protein is known to traffic to
of this protein in transgenic mice leads to alterations in
mitochondrial appearance and, ultimately, damage to heart
muscle and cardiomyopathy (Omura et al., 2005). Similar
effects might provide a common pathway for host-cell
functional impairment operated by diverse viruses in differ-
ent host cells and should be considered where virological
explanations for cellular dysfunction are suspected, but
evidence for causation by one particular type of virus is
weak. This may be relevant for instance in suspected viro-
logical aetiology of chronic fatigue syndrome.
Asano, S., Honda, T., Goshima, F., Watanabe, D., Miyake, Y.,
Sugiura, Y. & Nishiyama, Y. (1999). US3 protein kinase of herpes
simplex virus type 2 plays a role in protecting corneal epithelial cells
from apoptosis in infected mice. J Gen Virol 80, 51–56.
Asano, S., Honda, T., Goshima, F., Nishiyama, Y. & Sugiura, Y.
(2000). US3 protein kinase of herpes simplex virus protects primary
afferent neurons from virus-induced apoptosis in ICR mice. Neurosci
Lett 294, 105–108.
Benetti, L. & Roizman, B. (2004). Herpes simplex virus protein
kinase US3 activates and functionally overlaps protein kinase A to
block apoptosis. Proc Natl Acad Sci U S A 101, 9411–9416.
Calton, C. M., Randall, J. A., Adkins, M. W. & Banfield, B. W. (2004).
The pseudorabies virus serine/threonine kinase Us3 contains mito-
chondrial, nuclear and membrane localization signals. Virus Genes
29, 131–145.
Cartier, A., Komai, T. & Masucci, M. G. (2003). The Us3 protein kinase
of herpes simplex virus 1 blocks apoptosis and induces phos-
porylation of the Bcl-2 family member Bad. Exp Cell Res 291, 242–250.
D'Agostino, D. M., Ranzato, L., Arrigoni, G. & 10 other authors
(2002). Mitochondrial alterations induced by the p13IIprotein of
human T-cell leukemia virus type 1. Critical role of arginine residues.
J Biol Chem 277, 34424–34433.
Geenen, K., Favoreel, H. W., Olsen, L., Enquist, L. W. & Nauwynck,
H. J. (2005). The pseudorabies virus US3 protein kinase possesses
anti-apoptotic activity that protects cells from apoptosis during
infection and after treatment with sorbitol or staurosporine. Virology
331, 144–150.
Gibbs, J. S., Malide, D., Hornung, F., Bennink, J. R. & Yewdell, J. W.
(2003). The influenza A virus PB1-F2 protein targets the inner
mitochondrial membrane via a predicted basic amphipathic helix
that disrupts mitochondrial function. J Virol 77, 7214–7224.
Goshima, F., Daikoku, T., Yamada, H., Oshima, S., Tsurumi, T. &
Nishiyama, Y. (1998). Subcellular localization of the US3 protein
kinase of herpes simplex virus type 2. Arch Virol 143, 613–622.
Henkler, F., Hoare, J., Waseem, N., Goldin, R. D., McGarvey, M. J.,
Koshy, R. & King, I. A. (2001). Intracellular localization of the
hepatitis B virus HBx protein. J Gen Virol 82, 871–882.
Jerome, K. R., Fox, R., Chen, Z., Sears, A. E., Lee, H.-Y. & Corey, L.
(1999). Herpes simplex virus inhibits apoptosis through the action of
two genes, Us5 and Us3. J Virol 73, 8950–8957.
Fig. 3. Effect of US3 protein expression on respiration. (a)
mRNAs for US3 and luciferase proteins were synthesized in vitro
and transfectedintoHeLa cells
described in the text. Six hours later, cells were harvested and
total cellular respiration was measured. First column, respiration
from control (non-transfected cells); second column, respiration
from luciferase (Luc) mRNA-transfected cells; third column,
respiration from US3 mRNA-transfected cells. Expression of
US3, but not luciferase, led to a profound decrease in cellular
respiration. (b) HeLa cells were mock-infected or infected with
US3 deletion-mutant virus or its wild-type progenitor and cell
respiration was determined by using an OE as described in the
text. Asterisks indicate samples with significant divergence from
control values: ***, P<0?001.
by using Lipofectinas
2158 Journal of General Virology 87
M. Derakhshan and others
Page 5
Koundouris, A., Kass, G. E. N., Johnson, C. R., Boxall, A., Sanders,
P. G. & Carter, M. J. (2000). Poliovirus induces an early impairment
of mitochondrial function by inhibiting succinate dehydrogenase
activity. Biochem Biophys Res Commun 271, 610–614.
Lee, J.-Y., Marshall, J. A. & Bowden, D. S. (1999). Localization of
rubella virus core particles in Vero cells. Virology 265, 110–119.
Leopardi, R., Van Sant, C. & Roizman, B. (1997). The herpes simplex
virus 1 protein kinase US3 is required for protection from apoptosis
induced by the virus. Proc Natl Acad Sci U S A 94, 7891–7896.
Munger, J. & Roizman, B. (2001). The US3 protein kinase of herpes
simplex virus 1 mediates the posttranslational modification of BAD
and prevents BAD-induced programmed cell death in the absence of
other viral proteins. Proc Natl Acad Sci U S A 98, 10410–10415.
Murata, T., Goshima, F., Daikoku, T., Inagaki-Ohara, K., Takakuwa, H.,
Kato, K. & Nishiyama, Y. (2000). Mitochondrial distribution and
function in herpes simplex virus-infected cells. J Gen Virol 81, 401–406.
Norkin, L. C. (1977). Cell killing by simian virus 40: impairment of
membrane formation and function. J Virol 21, 872–879.
Ogg, P. D., McDonell, P. J., Ryckman, B. J., Knudson, C. M. & Roller,
R. J. (2004). The HSV-1 Us3 protein kinase is sufficient to block
apoptosis induced by overexpression of a variety of Bcl-2 family
members. Virology 319, 212–224.
Okuda, M., Li, K., Beard, M. R., Showalter, L. A., Scholle, F., Lemon,
S. M. & Weinman, S. A. (2002). Mitochondrial injury, oxidative stress,
and antioxidant gene expression are induced by hepatitis C virus
core protein. Gastroenterology 122, 366–375.
Omura, T., Yoshiyama, M., Hayashi, T. & 12 other authors (2005).
Core protein of hepatitis C virus induces cardiomyopathy. Circ Res
96, 148–150.
Purves, F. C., Longnecker, R. M., Leader, D. P. & Roizman, B.
(1987). The herpes simplex virus 1 protein kinase is encoded by
open reading frame US3 which is not essential for virus growth in
cell culture. J Virol 61, 2896–2901.
Radovanovic, J., Todorovic, V., Boricic, I., Jankovic-Hladni, M. &
pathology in the liver of AIDS patients: clusters of mitochondria,
protuberances, ''minimitochondria,'' vacuoles, and virus-like particles.
Ultrastruct Pathol 23, 19–24.
Rahmani, Z., Huh, K.-W., Lasher, R. & Siddiqui, A. (2000). Hepatitis
B virus X protein colocalizes to mitochondria with a human voltage-
dependent anion channel, HVDAC3, and alters its transmembrane
potential. J Virol 74, 2840–2846.
Reynolds, A. E., Wills, E. G., Roller, R. J., Ryckman, B. J. & Baines,
J.D.(2002).Ultrastructurallocalizationoftheherpessimplex virustype
1 UL31, UL34, and US3 proteins suggests specific roles in primary
envelopment and egress of nucleocapsids. J Virol 76, 8939–8952.
Schwer, B., Ren, S., Pietschmann, T., Kartenbeck, J., Kaehlcke, K.,
Bartenschlager, R., Yen, T. S. B. & Ott, M. (2004). Targeting of
hepatitis C virus core protein to mitochondria through a novel C-
terminal localization motif. J Virol 78, 7958–7968.
Takada, S., Shirakata, Y., Kaneniwa, N. & Koike, K. (1999).
Association of hepatitis B virus X protein with mitochondria
causes mitochondrial aggregation at the nuclear periphery, leading to
cell death. Oncogene 18, 6965–6973.
Yamada, H., Chounan, R., Higashi, Y., Kurihara, N. & Kido, H.
(2004). Mitochondrial targeting sequence of the influenza A virus
PB1-F2 protein and its function in mitochondria. FEBS Lett 578,
Herpesvirus mitochondrial respiration inhibition


text:
The Style Gallery is a great new way for you to view, love, and share outfit photos. It's meant to showcase
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Whether you've indeed flown through the air with the greatest of ease or simply want to show your affinity for beautiful clothes, this embroidered blue top is a surefire way to step your style up to the plate! Bend the rules a bit in this silky, sheer-backed, sleeveless shirt, and take it's exposed zipper, high-low hem, and racerback cut to a modern dance performance. This top's black stitching supports sleek silhouettes of performers hanging over your printed denim and black booties. Without a doubt, this top's web of lithe acrobats will alert everyone to your unique fashion sense and will have you flipping for its rich hue and graceful, playful feel.
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Take A Trip Inside Brunello Cucinelli's Utopian Village
Written by Skylar Bergl
Tuesday, July 28th, 2015 at 10:42am
We love Brunello with a large portion of our hearts. His product, ethics and ethos are all extremely laudable. Like, for real, he's easily one of the best dudes in the industry. He's also a huge proponent of work-life balance,


text:
How to Turn in a Drug Dealer
News you can use in Crown Heights. (Get it? "Use"?)By Ben Mathis-Lilley
News you can use in Crown Heights. (Get it? "Use"?)By Ben Mathis-Lilley
Getting foreclosed upon could be the best thing to happen to Victoria Gotti.By Jessica Pressler
It's different.By Emma Pearse
How the battle of wills between real-estate vultures and steadfast property owners will help the market.By Hugo Lindgren
Obviously she can't just move into the place without totally gutting it first.By Jessica Coen
We sent our Tim Murphy to the far reaches of the Upper East Side to meet the men and women who will be getting to know the pop star in the coming months.By Jonah Green and Tim Murphy
Uh, would that really surprise anyone?By Dan Amira
It would appear you've been double-parked.By Jessica Pressler
Some tour group is suckering New York visitors into going on paid guided tours of real-life locations from the show!By Chris Rovzar
In a day brimming over with terrifying pandemic and economy-related news, hope has sashayed into our cubicle.By Jessica Pressler
In the Hamptons, you can't destroy 3,000-year-old protected dunes without a fuss that you have to at least pay lawyers to attend to.By Chris Rovzar
Home prices in New York have dropped more than those of any other major city. We love it when we come in first place.By Jessica Coen
That is, it's plummeting at a rate even faster than Ludicrous Speed.By S. Jhoanna Robledo
That's right near where Liz Lemon lives!By Chris Rovzar
Watch the video to see the Gloria Vanderbilt room.By S. Jhoanna Robledo
Not everyone likes his little Bleecker Street empire.By Amy Odell
But at the end of the quarter, there was a promising uptick.By S. Jhoanna Robledo
Or will kids go there to buy drugs no matter what the layout of the park?By Chris Rovzar
But that picture of her with little Mercy James? Priceless.By Chris Rovzar
Rents are starting to drop all over the city. Can you take advantage?By S. Jhoanna Robledo


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|Search | About | Preferences | Interact | Help
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by Mike Venezia
Publisher:Childrens Pr
Author/Illustrator Mike Venezia has been getting to know the world's greatest artists for as long as he can remember. A graduate of The School of the Art Institute of Chicago, Mike believes the best way to introduce children to art and artists is through fun. If children can look at art in a fun way, and think of artists as real people, the exciting world of art will be open to them for the rest of their lives.
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Did you know that the Library offers fun and practical literacy programs for adults? We provide free courses to everyone in our community, helping Waukeganites reach their goals through computer and internet classes, adult education in reading, math, and job skills, as well as English language learning. It's all made possible by our incredible volunteers!
We're especially in need of volunteer Adult Literacy Tutors. Our tutors work with adult students on a variety of subjects, from reading to math to English as a second language (ESL). Many of the Library's adult learners come to our program because they want to improve their employment opportunities or play an active role in their children's education, but we also serve learners who want to improve their skills purely for fulfillment and the satisfying achievement of continuing education. We also provide hands-on, practical ESL tutoring; our tutors don't just help their students become more conversational in English, they help them to navigate daily life in a new language by providing opportunities to practice skills in real-life settings. In so doing, our tutors form deeply meaningful relationships with their students. They often say they gain just as much as their students, if not more, from tutoring!
Training, materials, and support are provided by the Tutoring Program staff, so novice tutors looking for a new volunteering experience are especially welcome! Our Adult Literacy Tutors training program occurs over two consecutive Saturdays. The next training is scheduled for August 8 and August 15, 2015. You can learn more about the program here.
We're also in search of volunteers to assist in our Digital Literacy classroom courses, helping students as they learn about computers and the Internet, hone essential job skills and develop financial literacy. Volunteers for these courses will have their own orientation, with timing to be determined.
We're looking for adults who are passionate about putting their expertise and talents to work making a difference in our city. Ready to get involved? Click here to visit our Volunteer Match page, or call Rachel Cope at (847) 775-2547.


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20 August 2014
Brain drain: Are we evolving stupidity?
Has our century-long rise in intelligence gone into reverse? (Image: Oliver Jeffers)
We've got smarter and smarter in the 20th century, but now there are signs that IQs have begun to fall in countries such as the UK and Australia
IN DENMARK, every man is liable for military service at the age of 18. Nowadays, only a few thousand get conscripted but all have to be assessed, and that includes doing an IQ test. Until recently, the same one had been used since the 1950s. "We actually have the same test being administered to 25 to 30,000 young men every year," says Thomas Teasdale, a psychologist at the University of Copenhagen.
The results are surprising. Over this time, there has been a dramatic increase in the average IQ of Danish men. So much so that what would have been ...


text:
Dolphin art piece Dolphin jumping through a hoop The dolphin is often described as humane animal The dolphin is a symbol of the joy of life
This great product in white from our department Men and T-Shirt category can be printed just for you in the size and style you like. The design Dolphin jumping through a hoop was created by namo and uploaded to Spreadshirt for you to have printed on a T-Shirt. Next to a host of other great designs, you can find this Dolphin jumping through a hoop design in Spreadshirt's Marine Life category. If you are interested in topics like Flipper or Love, then you'll love Dolphin jumping through a hoop designs by namo. Spreadshirt also offers a huge selection of personalised T-shirts, hoodies and accessories for the category Marine Life as well as top Rescue clothing. namo's designs have already impressed many of our customers, so we are sure that we've got right stuff for you! Spreadshirt is your shop for great designs of all kinds – whether it's Flipper, Love or Rescue. We are sure that you will find something nice in our shop, there are many amazing personalised Marine Life T-shirts, jumpers or accessories for you to find! Tags: Design ID: 13121267
Mens Black Piston Shirt Designed by Hi-Artz Press
Diablo Road Runners - Foo Fighters Designed by mackenteeUK
Melting Rubiks Cube Designed by Rubiks Cube
Robot City Skyline T-Shirts Designed by no-go
Simple Bow Tie & Shirt Buttons T-Shirts Designed by N36
Fuck Verb FAAK Designed by 3n19ma
South African Springbok 2 T-Shirts Designed by Magnaen
Tartan Army Girls Scotland Football Designed by BonnieScotland
Installing Muscles (Loading) T-Shirts Designed by macello
She´s Mine (Comic Hand) T-Shirts Designed by macello
Good for you and the environment: This T-shirt is made from organic threads stemming from ecologically sound production. Part of the "Earthpositive Collection" by Continental Clothing, it has been produced with renewable energy sources alone.
still to fill


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Faculty & Staff
Kui Du, PhD
- Assistant Professor of Management Science and Information Systems
- 617.287.3171 Telephone:
- firstname.lastname@example.com Email:
100 Morrissey Blvd. Office Location: M-5-203
Areas of Expertise
Strategic Management of Information Technology; IT management during corporate transactions (Acquisitions, Divestitures, Spin-offs); Healthcare IT
Ph.D., the University of Texas at Austin
M.A., Peking University, China
B.E., Beijing Information Technology Institute, China
Professional Publications & Contributions
- Du, Kui and Tanriverdi, Huseyin, 2014, "Managing Information Technology under Extreme Organizational Disequilibrium: The Case of Corporate Spinoffs," in the Proceedings of 31st International Conference on Information Systems (ICIS), December 2014, Auckland, New Zealand.
- Du, Kui, 2013, "Digital Accommodation during Corporate Acquisitions," in the Best Paper Proceedings of the 73rd Annual Meeting of the Academy of Management (AoM), August 2013, Orlando FL.
- Du, Kui and Tanriverdi, Huseyin, 2010, "The Performance Effects of Transitional IT Services in Corporate Spin-offs," in the Proceedings of 31st International Conference on Information Systems (ICIS), December 2010, St. Louis, MO.
- Tanriverdi, Huseyin and Du, Kui, 2009, "Disintegrating Information Technology in Corporate Divestures: Implications for Regulatory Compliance Risks and Costs," in the Proceedings of 30th International Conference on Information Systems (ICIS), December 2009, Phoenix, AZ.
Additional Information
Dr. Kui Du primarily works at the intersection of information technology and strategic management. His overarching research interest is to understand how increasingly commoditized IT can still contribute to differential firm performance, and some of his most recent work focuses on the transforming role of IT in healthcare organizations. Prior to pursuing his PhD degree, he worked as an IT consultant at BearingPoint and an auditor at PricewaterhouseCoopers.
Courses Taught
Introductory MIS, Database Management, Object-Oriented System Development, Healthcare Analytics
Professional Experiences
Worked as an auditor at PriceWaterhouseCoopers and an IT consultant at BearingPoint.


text:
Amherst Inn
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this b&b rated in the past?
- Continental Breakfast
- Free High-Speed Internet
- Business Services
- Free parking
Travelers also recommend:
TripAdvisor Reviews Amherst Inn
Travel Blogs from Amherst
Day Two
We got on the road at 8am. It was a beautiful morning. The landscape through central Pennsylvania is so pretty; lots of hills and forests. We don't think we have driven on I80 through Pennsylvania before and we enjoyed the ride.
We get into discussions sometimes and forget to get off the exit we are supposed to. Last night we missed our exit to the campground and drove 10 miles father than ...
Juy 30th - Jellystone
... Sure enough, the rain
it for an hour causing a mini river outside the RV.
The other thing on the schedule was
Bingo. Never played that as a family before so why not. It was still
on despite the rain so we walked over with umbrellas. They decided to
hold it outside when the rain stopped briefly and we were handed
paper towels to dry off the tables and chairs. It started raining
lightly again part way through but we got ...
Borders banks and burgers
... allergies while you are a road trip but no harm no foul. The border was shockingly quiet, in that we were the only vehicle there. The border guard looked and sounded a bit like Nicholas Cage, so I was trying really hard to not giggle. He had Bo get out of the car with him and open the trailer so he could have a quick peek. Unfortunately I didn't know it, but you are not allowed to bring bell peppers over the border so they had to be discarded ...
Next, Class V Rapids?
... the hill into the water. Several people go first, with the instructor pushing then down the hill. Of course, being and experts will skills that far exceed these amateurs, I let her know I will not be needing a push. So she says, do you want to do it over there, down the rocks? This is a steeper part, so of course I say yes. She then indicates, cool, I have never seen anyone try it there before, I just don't want you to get hurt. Ha, ...
Cool, grey, and scattered showers
... scolded for going to the wrong register. Beer and wine are at one and booze is at another. Who knew. I am staying at an expensive hotel. The price quoted was $170. When I said that was out of my league, the clerk offered it at $97. What a wonderful person. I am doing laundry on the clothes that were affected by the shampoo ...


text:
Health IT
A profusion of data from sensors, home monitoring devices and health and wellness apps threatens to overwhelm medical providers. The Veterans Administration is trying to get ahead of the problem with a playbook on how to build applications that collect data, structure information, establish rules on when and how to share that data with clinicians and integrate that data into existing and future electronic health records.
"Patient generated data is going to be the thing that transforms health care," Kathleen Frisbee, co-director of Connected Health at the Veterans Health Administration Office of Information and Analytics, said in keynote remarks at the Government Information Technology Executive Council annual summit in Baltimore. "We predict that patient-generated data will be much larger in volume than electronic health care records," she said.
VHA is trying to get a grip on the data by taking a firm hand in guiding the application development and management process. There is an app store in the works, that's more that just a holding page. The Terremark-hosted application development cloud is a repository for all public-facing apps, because VA is insisting on doing continuous security scans on apps through development, testing, production and updates. Additionally, apps are reviewed for patient safety and privacy concerns and for their clinical utility to the populations served by the department. Each app passes through 16 review bodies before a pilot can go live.
"If you want your VA app using the VA license, you have to have the code there," Frisbee said.
A few recent VA pilots are getting close to their official release.
A set of apps called the Family Caregiver Suite helps family members caring for vets track pain management, post-traumatic stress disorder symptoms, prescription refills and more. According to early testing, the apps are more popular in rural areas. Older veterans were less likely to use the apps, and Frisbee linked this to the VA's use of the Department of Defense Self-Service Access Center credentialing system, known as the DS-Logon, to access the system. Younger vets have the credential, but it can be complicated for an older vet to obtain it.
The VA is also piloting a text-message alert system called Annie, named for Annie Fox, the first U.S. military nurse to receive a Purple Heart. It is a two-way communication system between a provider and patient that reminds patients to send in diagnostic information, take medicine and fulfill other aspects of ongoing medical protocols.
Another pilot in the works involves a wearable patch that continually sends patient biometric data. The challenge here and with other device-based data feeds is how to summarize the data for clinicians, and when and how to alert providers to changes.
"We're not going to alert our providers to death," Frisbee said. Instead, the VA is planning to write rules that spell out when providers are required to look at data, and how to make the data available during VA appointments, emergency room visits and visits with providers outside the VA network.
Currently, there is a gap between data fed by patient-based apps and sensors and the design and architecture of the current VA electronic health record.
That presents two big challenges: understanding how data collected from apps fits inside the electronic health records, and designing for the future. "Everything we're doing in mobile has to converge with the new electronic health records that VA is building," Frisbee said.
About the Author
Adam Mazmanian is FCW's executive editor. Connect with him on Twitter: @thisismaz.
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In the newest Federal Employee Viewpoint Survey, feds show they're feeling better than they have over the past few years – but there's still room for improvement, especially in mission-critical IT.
The General Services Administration's Enterprise Infrastructure Solutions contract will anchor the agency's NS 2020 telecommunications strategy.
House and Senate both approve a measure to maintain funding at fiscal 2015 levels through Dec. 11.
The aviation regulator missed a Congressional deadline to develop rules of the sky-ways for drones.
Agency heads, acquisition officers and even CIOs don't need to be hard-core techies. FCW's four-part look at the expertise that's really required to make federal IT run.
FCW recognizes 14 individuals having an outsized impact early in their careers.
As Steve Kelman returns from a summer of aggressive medical treatment, he considers the all-too-common aversion to short-term sacrifices.
Massive open online courses can offer specialized IT instruction on a flexible schedule and on the cheap. That may not always mesh with government's preference for structure and certification, however.
The federal government craves more cybersecurity professionals. These six schools are helping meet that demand.
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We're only a couple weeks away from losing the Eleventh Doctor! SADNESS. Losing your favorite Doctor can be a very traumatic experience,...More →
Okay, we know that's a lot to wrap your head around, but believe us when we tell you that it's worth a...More →
With the 50th Anniversary having just gone by, and the farewell to Matt Smith as the Doctor just over the horizon, we...More →
Over the years Doctor Who has used the holiday season as a backdrop for many unforgettable episodes and every year Whovians get...More →
Looking for a gift to make the day of the Doctor Who fan in your life? If they already have all the...More →
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While the wannabe Time Lords of Earth adjust their bowties, check the batteries in their sonic screwdrivers, and decide where to watch...More →
The Doctor Who 50th Anniversary special is almost here! Over the years we've watched eleven Doctors travel time and space, but we've...More →
The BBC is just chock full of surprises, because yesterday, like a Tardis emerging suddenly from a wormhole, they dropped an unannounced...More →
Barry Wazzy isn't known as "The Pokeball Guy" for nothing. Over the years, this Aussie artist has amassed an army of Pokeballs that's...More →


text: I'm sitting here reflecting on my ob visit today. I had bad cramps and diarrhea, so at the appt the ob checked for bacteria and to make sure my cervix was still close and the bad cramping was not from the uterus. He did this manually! I have had the spetulum(sp) cervix check, but never this except for when I get a regular annual. I'm glad he said everything felt okay... But now I'm seeing that these checks are bad and can cause PTL or other stuff!!? Idk I like this ob office, but am kinda concerned... anybody else had to have a manual (hand) cervix check between 22-26 weeks and were okay?


text:
Sound of opening drawer.
A treasure chest being opened.
unwrapping candy
Simple magic sound. Made in FL Studio.
Cartoon sounding snore that is fast and short.
A coin sound. useful for game devs. made using sfxr.
A wonderful looping bell sequence with 5 different octaves. Deep and tense. Peace.
A cheerful sound for selecting start on a game menu! ... Or whatever you want to do with it. Made ...
waving a twig Recorder: ZOOM H2 / other portable device Software: Pro Tools / Audacity / Ocenaudio
Breaking a glass bottle
Clinking a wine glass with a knife three times to get attention at a dinner table
one single ring of a set of indian finger cymbals. good for when angels get their wings. :)
Semi tuned bell tones. All tones are derived from one bell.
small bell chiming 3 times. Dzwonek medytacyjny dzwoniący trzy razy.


text:
Anyone have this? Having trouble with the other links.
"Jim's a tough guy and you can see his personality is all over this football team," Fitzgerald said.
"Jim's a tough guy and you can see his personality is all over this football team," Fitzgerald said.
a terrible blight on our fine country
point UFR
who fails upward better: Whitlock, Kiffin, or Brandon?
"He makes it really easy on you as a coach because he has tremendous football instincts," Michigan tight ends coach Jay Harbaugh said. "Things come really naturally to him. He doesn't have to see things too many times. He has a good sense for how things should look and feel, and he's a tough, physical guy."
this week in dead men walking
"They called Brigham Young the American Moses, so I see why the offense died without reaching the end zone."
To be eligible for the award, a student-athlete must be in his final year of eligibility, hold at least a 3.2 grade-point average and "have outstanding football ability as a first team player or significant contributor and have demonstrated strong leadership and citizenship."
"That was one of those plays that was real contact courage," Harbaugh said of Chesson's block. "He just went and made a real, hearty block. I was happy to see that. Darboh is doing the same thing, and Ways is doing the same thing at a higher level than most receivers you're ever going to find."
Tennesee hires Mike DeBord, suddenly starts losing games with huge fourth quarter leads. Dude is the Murder She Wrote of football.
"The Wildcats' endzone might as well be the moon; sure it is possible to go there, and it's been done in the past, but opposing teams are wondering if they have the manpower and the short-sleeved white button-down shirts to engineer a way there and how are they going to convince the government to give them the resources to try in this economy."
Pretty sure BTN is trolling these boards looking for links to shut down. If you know it find a way to private message and don't public post it.
can someone privately message me the Justin.TV access code for the game? This is the first UM game I am missing in 12 years because of a family function, and I only have access to the internet. Die hard fan here, and really would like to watch the game!
"An Army is a team; lives, sleeps, eats, fights as a team. This individual heroic stuff is a lot of crap."
- Gen. Patton
can someone PM me the password?
Can someone hook me up??


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text:
Better Business Bureau Report issued
October 2013
Better Business Bureau Report expires October 2015
Does not meet one or more standardsThis charity does not meet one or more of the 20 standards for Charity Accountability. Find out more...
Year, State Incorporated: 1972, Illinois
Affiliates: None
Stated Purpose: To present the living Christ, primarily to unchurched inner city children and youth, discipling and integrating them into a local church.
Standard 8: Program Service Expense Ratio - Spend at least 65% of its total expenses on program activities.
Inner City Impact does not meet this Standard because:
Standard 16: Annual Report - Have an annual report available to all, on request, that includes: (a) the organization's mission statement, (b) a summary of the past year's program service accomplishments, (c) a roster of the officers and members of the board of directors, (d) financial information that includes (i) total income in the past fiscal year, (ii) expenses in the same program, fund raising and administrative categories as in the financial statements, and (iii) ending net assets.
Inner City Impact does not meet this standard because:
Standard 17: Web Site Disclosures - Include on any charity websites that solicit contributions, the same information that is recommended for annual reports, as well as the mailing address of the charity and electronic access to its most recent IRS Form 990.
Inner City Impact does not meet this Standard because the organization's website, www.Innercityimpact.org, does not include all of the recommended information for those charity websites that solicit for donations. Specifically, it does not include:
An organization may change its practices at any time without
notice. A copy of this report has been shared with the organization prior to publication.
It is not intended to recommend or deprecate, and is furnished solely to assist
you in exercising your own judgment. If the report is about a charity and states
the charity meets or does not meet the
BBB Standards for Charity Accountability, it reflects the results of an
evaluation of information and materials provided voluntarily by the charity. The
name Better Business Bureau is a registered service mark of the Council of Better
Business Bureaus, Inc.
This report is not to be used for fund raising or promotional purposes.
Send to:


text:
Welcome to DPI/AP
Request to help our Flood Victims with disabilities and their Families in Myanmar
Help Flood Victims with Disabilities and their Families in Myanmar
Dear Colleagues and Friends,
Greetings from Myanmar Independent Living Initiative-MILI (Disabled People's Organization).
Myanmar people need your attention and support especially for Persons with disability and their Families.
As you all known that Cyclone KOMEN and heavy torrential rains recently brought unprecedented large scale flooding and landslide all over the Myanmar since from late July, 2015. With this severe crisis, it is estimated that over 300,000 people in middle and west part of the country have affected by the Flood: many of them have lost their homes & properties and some of them have died. Currently, some Myanmar CSOs and International NGOs are again at the frontline of the rescue and humanitarian aid, and they are struggling to access flood-hit areas. Humanitarian aid is much urgently needed to save the lives.
Many people are terribly suffering this crisis. Persons with disabilities and their families are also same with this suffering. However, according to our past experiences in disaster management, we found that many disabled persons are still left behind from emergency assistances and humanitarian aids due to several barriers and difficulties. With this lessons learnt, we, MILI has decided to take actions in these areas to save the lives of households with disabilities and their communities.
• To provide food and emergency assistance to flood affected people with/without disabilities
• To facilitate the disability inclusive emergency response and humanitarian aid programs
Targeted beneficiaries: 1,200 Households with disabilities and their community members
Targeted geographical areas
One Township in Sagaing Region 200-Households will be supported
One Township in Magway Region 200-Households will be supported
One Township in Chin State 200-Households will be supported
Two Townships in Rakhine State 400-Households will be supported
One Township in northern of Yangon Region 200-Households will be supported
Steps and Activities which will be undertaken and How
• Make contacts with MILI's branches in target areas and coordinate for emergency relief
• Send the staffs and volunteers to target areas and work together with branches for data collection and beneficiary selection
• Distribute and deliver the emergency assistances door-to-door (include food, drinking water, clothes, tarpaulins, medicines, assistive devices, utensils and sanitation pads)
• Evaluate on done activities and
• Make a report
Coordination and Collaboration
MILI will closely coordinate with its' branches and additionally collaborate with government: particularly general administrative department and relief & resettlement department, UN Agencies, International and Local NGOs and CSOs in target areas.
Focal contact person of MILI
Nay Lin Soe, Program Director
Phone: 0943-123-742
Email: firstname.lastname@example.com , firstname.lastname@example.com
DPI General Assembly 2016
First Announcement
Dear National Council members,
It is a pleasure of the special committee in charge of organizing the coming general assembly of DPI to declare that the World Assembly is to take place in 19th -20th March 2016 in Beirut (Lebanon).
Main theme of the General Assembly: Disability and post 2015 SDG.
Please confirm your participation to Mr. Jean Luc Simon, committee secretary, with the required document for participation.
Jean Luc Simon
Disabled People's International (DPI)
C/O: Groupement des Personnes Handicapées (GFPH)
45, Rue Riquet, Appt. 664, 75019 , Paris, France
Telephone: +33(0)1-40-05-10-90 / +33(0)6-12-92-90-53
Email: firstname.lastname@example.com
Website: www.dpi-europe.org / www.gfph.dpi-europe.org
Letter of Announcement DPI General Assembly 2016 Download the document
The new DPI leaders
Message from the DPI Chair, July 2015
The DPI World Council Members for having elected me as the Interim Chair at their extraordinary World Council Meeting which was held in Dhaka, Bangladesh from June 28- June 30th 2015, in order to restore the dignity and lost glory of our beloved organization (Disabled People's International DPI) on the basis of our shared values and principles, which are democracy and mutual support in respect of each one whatever his/her abilities, origin, gender, religion or social appurtenance.
The DPI member BPKS and the people with disabilities from Bangladesh who mobilized their resources to host this meeting in Dhaka within 29 days, I would like to sincerely thank them for having organized everything including food and accommodation for the participants in an efficient manner.
Decided on May 28 and noticed on May 29 and 30 to all the World Council Members elected by the 6 regional Assemblies, this extraordinary meeting is a clear demonstration of our effort and ability to decide, to act and to engage changes. I would like to also acknowledge and commend the World Council Members from 9 countries who made their travel expenses from their own resources, and 10 others who sent their proxies with their proposals for the future of DPI and with 3 who had participated in the discussions through Internet Communications Systems.
This challenge was followed by the adoption of a clear and determined statement and a list of decisions that mark a new future for our dear organization, and an action plan to reaffirm our common values and to lead DPI through to its next World Assembly. DPI will endeavour to lead disabled people around the world to their inclusion in society through lobbying and advocating for the implementation of the UNCRPD and all other international and national instruments for the building of a sustainable, peaceful and better world for all. DPI will ensure transparency and accountability in all its undertakings/dealings.
Rachel Kachaje
Interim Chairperson of DPI
More information http://dpi-europe.org/
Deep Condolences to Mr.Frank Mulcahy
We are saddened to report the death of Mr.Frank Mulcahy passed away on Sunday, 19 July. 2015 at his home and surrounded by his family,
As many of you know, Frank Mulcahy has been historically one of DPI's greatest leaders, to whom we are all indebted for his major contributions, both for the establishment and functioning the European Disability Forum and the CRPD
On behalf of Disabled Peoples' International Asia-Pacific Region. We thank God and Frank's family for such a wonderful gift to the disability rights in European and beyond.
Abidi removed from DPI post
Indian disability rights activist Javid Abidi has been removed as the chair of Disabled People's International (DPI) in a "DPI World Council extraordinary meeting held in Bangladesh late last month."
A statement issued by the Council noted: "We have deep concerns about the unity, status and image of DPI as a constitutional, transparent and democratic organisation and strongly believe that this image has been damaged as a result of the autocratic leadership style of DPI chair Javed Abidi."
Listing out the reasons for the move the Council noted that most decisions in the name of DPI have been taken unilaterally by the chair without any approval by the World Council and consultation with the executive officers. "Among others, this includes closing of the official seat of DPI in Canada and running the organisation by a non-DPI staff in Delhi and without an executive director. The dismissal of DPI executive director without the consent of the world council as stated in the Constitution that has caused damaging effects on DPI work and prestige and relationship with major funders."
Abidi's unconstitutional intervention in countries where DPI is established by revoking elected leadership and replacing it by a new one were also detrimental noted the Council.
The World Council has decided to elect DPI vice-chair Rachel Kachaje as a DPI acting chair for an interim period until the coming world assembly where the election of a new chair will take place.
source www.thehindu.com


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STARmeterSEE RANK
Up 228 this week
Doug McKeon
Doug McKeon was born on June 10, 1966 in Pompton Plains, New Jersey, USA. He is an actor and director, known for On Golden Pond (1981), I Spit on Your Grave: Vengeance is Mine (2015) and Mischief (1985). See full bio »
Born:in Pompton Plains, New Jersey, USA
More at IMDbPro » Contact Info: View agent
2 photos | 3 videos | 11 news articles »


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ldar Companies in Bosnia & Herzegovina
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based in Louisville, KENTUCKY (USA)
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Engineering, Studies and Projects NIP,S.A. is an engineering and consulting company with the purpose of providing a high quality flexible technical service that can respond to our clients' needs to obtain their complete satisfaction. Today NIPSA is found in sectors such as: Energy, Water, Telecommunications, Environment, Industry, Airports, Mapping and information systems ...
Custom Stack Analysis, LLC.
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Calvin Consulting Group Ltd.
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WKC works primarily on projects based within the Middle East Africa and Europe; our worldwide network of consultants and associates also allows us to put competitive teams together to meet our client`s global needs. WKC`s aim is to assist our clients in meeting their environmental and sustainability responsibilities, and achieving compliance with the environmental ...
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The Lamb Inn Burford, Cotswolds
Quintessential Cotswold Inn set in the popular market town of Burford
OPL Exclusive details
Midweek Romantic Hideaway
From £71.25 pppn for a 6 night stay, to £102.50 pppn for a 1 night stay
Stay in a Double room and receive:
- 1/2 bottle of Champagne and box of chocolates in your room on arrival
- Three course candle-lit dinner for two on the first night
- Full English breakfast
- Please contact us on 0845 287 1007 for twin room rates
There are 4 special offers to choose from for this hotel.
View all offers?
- Picture-postcard traditional Inn with luxury rooms
- Located in the heart of the bustling Cotswold market town of Burford
- Charming country style rooms
- Award winning restaurant
- Inviting bar with log fire and comfy chairs to lounge in.
- Dog friendly
Home truths
From the outside and indeed as you enter this hotel it looks more like a small country pub - but looks can be decieving. Tucked away to the rear is an extensive and superb retaurant, a courtyard area, and access to the 17 bedrooms
How to find The Lamb Inn
The Lamb Inn
Sheep Street
ox18 4lr
Good to know
Hotel check-in time:3:00 PM
Hotel check-out time:11:00 AM
Opening Times
7.30am-10am daily
12pm-2.30pm daily
7pm-9.30pm daily
Bar menu
Afternoon Tea
3pm-5.30pm daily
Dress Code
(No Jeans or Trainers)
Parking Facilities
Free street parking out the front and to the side of the hotel. Parking also available at The Bay Tree Hotel next door (sister hotel to the Lamb Inn).
Disabled Access
We have 3 ground floor rooms, there are some steps on route to the bar, lounge and restaurant.
Dogs Policy
Allocated rooms only
Explore elsewhere
Hotel collectionsClassic Country Food Lovers Pooch Perfect
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Booking Conditions
Other UK hotels that may be of interest
Between Witney & Burford
You can book any hotel break through our secure online booking system.
If you have any queries please check our FAQs. We accept the following credit and debit cards:


text:
Dr. Brent Culver specializes in rheumatology, internal medicine in Santa Cruz, California.
Dr. Brent Culver has been compared 55 times. Details of Dr. Culver's 17 years experience as an MD and education at University of California are available on your free doctor report.
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Santa Cruz Main Clinic
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Dr. Brent Culver has been identified as specializing in Rheumatology and Internal Medicine.
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IT needs to be slow cooked for about an hour.This version has been tamed down a bit, add more chilies if you like it with more heat.
Leave a Review
As has been noted, this has been ripped off from Madhur Jaffrey. Anyhow, it's a pretty good recipe. I find that it's not always necessary to blend the onions to a puree ¿ I just slice them very thin using a mandoline and then cook them until they're nice and crisp. This whole thing cooks for over an hour, so the onions are completely dissolved by the end anyway. I also use red onions and not yellow ones (I grew up in India and we only get red onions there...). I don't know why it would take someone 4 (!!) hours to brown onions. You can speed it up by slicing them *very* thin (a mandoline is indispensable for this) and cooking on high heat in a non stick pan to prevent any burning/sticking. I use ghee rather than oil; it's tastier, I find. And I've also made this with beef and it's excellent. (I boneless chuck roast). For the vinegar ¿ I've often uses a blend of vinegars (rice, lemon, balsamic) when I've been out of white vinegar and I prefer it. Apple cider is good, too. Vindaloo was originally made with palm vinegar, but that's hard to find in the US. I would also add more than a tsp of sugar. As some other folks have noted, vindaloo is always better the next day, although it's pretty delicious right away too!flag if inappropriate
This recipe is stolen word for word from Madhur Jaffrey's 1982 book Indian Cooking. Lame that the submitter couldn't include the source. The original creator deserves credit. I understand that Jaffrey didn't invent pork vindaloo, but she definitely wrote this exact recipe in her book.flag if inappropriate
The longer you let this simmer, the better it will taste. The spices need time to melt together. I used just a tabplespoon of oil for cooking the onions and my family still loved it. I did add twice the brown sugar though. It is a dish i plan to make again, so i made three times the amount of the dry spice mix for future batches. Get the onions cooking on low heat before you do anything else. I did add some extra paprika to mine, just for the redness as my chili peppers are very spicy.flag if inappropriate
The longer it cooks, the better. If you undercook it, the spices wont have time to melt together. Fantastic recipe!flag if inappropriate
this is the best vindaloo recipe EVERflag if inappropriate
Fantastic recipe. One of the best Indian recipes I have ever tried -- and I've tried many. I've made this 4 or 5 times already. What I learned: 1. make sure the cubes are not too big -- I found about 3/4 inch was best; 2. Start frying those onions right away, first thing; 3. Sauteeing the onions in about 1/4-1/3 cup of oil seemed just fine to me; Just a wonderful way to enjoy the great taste of vindaloo for the US palate! and unlike other vindaloo recipes, there's no marinating. Thanks a bunch!flag if inappropriate
Simply wonderful! Rich, rich and deep flavors. Authentic. Surprised it took so long for some -- as Indian recipes go, it's not too complicated. The trick is to cut the onions and start them browning FIRST. Will make over and over.flag if inappropriate
To die for! Not sure I will deep fry the onions next time. Might just caramelize in a pan.flag if inappropriate
The recipe is great, though with all the steps and getting those darn onions to crisp took 4-5 hours. It is very good food though. The main thing is that if cooking for a large party, a lot of people have never had a real vindaloo so don't really know what it is supposed to taste like. The salty, sour taste is not to everyone's liking. If you like it, make the recipe.flag if inappropriate
I reduced the oil to a 1/4 cup by having onions ready for use that I had boiled and simmered down to a brown syrupy mixture (I make curry frequently and this is a necessary ingredient). I also used a harder apple cider vinegar. I appreciate that this recipe has no tomato puree - the vinegar serves as the sole souring agent. I cooked it for a few minutes in a pressure cooker which usually results in excellent curry. The masala mixture in this recipe smells superb - especially with the vinegar! I can't wait to sample...flag if inappropriate


text:
A special reception featuring remarks by Bruce Davidson and Senator Ron Wyden will take place at the Russell Senate Building, Room 385, on Tuesday, Feb. 10, from 5 p.m. to 7:30 p.m. (remarks will be made around 5:15 p.m.) The photos will be on display at the Russell Senate Building Rotunda from Feb. 9-13, 2009. By displaying pictures of actual CareOregon members, ACAP hopes to put a face to Safety Net Health Plans.
For more information about ACAP or any of their member plans, please visit www.communityplans.net.
|SOURCE The Association for Community Affiliated Plans (ACAP)
Copyright©2009 PR Newswire.
All rights reserved


text:
J by Jasper Conran Designer gunmetal photo frame
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Ratings based on 1,070 Verified Reviews
They paid and stayed. We double-checked.
4.4 Room cleanliness
4.4 Service & staff
4.3 Room comfort
4.2 Hotel condition
They paid and stayed.
We double-checked.
We double-checked.
for Everyoneby A verified traveler from northern CA
Served it's purposePosted Oct 5, 2015
Needed a room for a wedding in the area. The room was clean and comfortable bed. The only thing was we could here doors closing regularly. It was loud! Our room was on the end near a doorway so maybe we heard nor that others. The room was really big for a hotel room and bathroom counter was a big area too.
for Couplesby A verified traveler from Mures
Good motel for Yosemite surroundingsPosted Oct 3, 2015
Pros: Nice motel in Sonora outskirts, big room with comfortable beds, good complimentary breakfast.
Cons: The access to the rooms is via a concrete stairs and floor that are not very estetique.
Location: We went to the Yosemite, about two hours drive.
We stopped here on our way to Yosemite, for one night. Fast check-in, check-out. Room was hard to find, but we were also tired after our flight and not used to the american motel system (to drive from reception to the building with the room using a map).
for Everyoneby A verified traveler from Tracy, CA
A great hotel to stay at when visiting SonoraPosted Sep 28, 2015
Pros: Staff, amenities, location and good value.
Cons: Believe it or not, only fault was TV remote control. It sucked! It had to be pointed directly at TV and would not work at even smallest side angles.
Reception desk staff were courteous, efficient and funny (a compliment). Room was clean and comfortable with good amenities ... wifi, complimentary breakfast, microwave and fridge.
for Everyoneby A verified traveler
Posted Sep 22, 2015
Our stay was quiet, safe, and the staff was excellent. The room was spacious and clean!
for Everyoneby A verified traveler
Nice place. The free breakfast read great.Posted Sep 19, 2015
When we arrived in our room, the bathroom light didn't work. I called the desk and they sent someone to fix it. Also, they didn't replenish the regular coffee.
for Everyoneby A verified traveler
Lekker bed, mooie kamerPosted Sep 18, 2015
Hadden een heerlijk bed en ruime kamer. Ontbijt erg goed, keuze uit 4 verschillende gerechten. Top!
for Everyoneby A verified traveler from Denmark
Godt hotel til miniferiePosted Sep 4, 2015
Super godt værelse ud til pool område med egen terasse. Happy hour tirsdag med gratis øl/vin og aftensmad. Egen morgenmadsrestaurent med stort udvalg. Ligger i rolige omgivelser tæt på western byerne Jamestown og Colombia.
for Everyoneby A verified traveler from Kansas City
Posted Sep 2, 2015
Comfortable and clean rooms with microwave and refrigerator. Choice of 4 complimentary breakfast options in the hotel diner. Would stay again.
for Everyoneby A verified traveler from SF Bay Area
Quiet Stay in SonoraPosted Aug 31, 2015
Stayed one night on our way to Yosemite. Spotless room, reasonable rate, breakfast at adjacent restuarant included. AC was a little noisy. Fitness center good.
for Everyoneby A verified traveler from Reading, UK
Amazing hotel!Posted Aug 30, 2015
Pros: Everything.
Cons: Warmer pool.
Location: Close to the freeway. One hour's drive from Yosemite.
Our room was very clean and luxurious and had a lovely balcony with a table and two chairs which overlooked the pool. We used the pool twice during our stay and it was very nice although a bit cold, and the hot tub was wonderful. The included breakfast was provided by the restaurant next door, and guests could choose one of several options from the menu. This was very nice although we would have liked to be able to choose what we wanted specifically. This option also took a lot longer than just having a buffet. We also chose to have dinner at the restaurant, and that was good too.


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Posted July 18, 2010
Arkansas vs. LSU
Demarcus Love
14:30 1st - Bends at waist too much and on ground
below average footwork - crosses over with long steps
Quick hips and feet on angle block
good leverage, natural knee bender, keeps feet moving
Footwork needs polish ... long steps still crossing over ... very good agility and smooth athlete
excellent job picking up the blitz, plays with good speed, but footwork needs to be better
Blocking on heels, not balls of feet...gets a nice hand punch + good strength
Mallett: Strong arm, poor mechanics and footwork, not mobile and a bad athlete... Ball tends to sail - releases too early
needs to work on timing ... ball not coming out early enough
Barsdale - possibly missed assignment on blitz...bad vision/awarenes? ... has heavy hands...
Misses blocks again
natural knee bender and keeps butt low ... elite technician
Kelvin Sheppard: solid in pass coverage
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Andrew Luck vs. Jake Locker
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Now - Test your tactics against legendary foes in this critically acclaimed Warhammer iOS game! | Promoted post
Wiiloveit.com: Samurai Sword Destiny - 3DS Download Review
1272d ago - "The hack-'n'-slash genre of games has been widely panned, being judged simply as nothing more th... | 3DS
TLR: Diss or Download Video Review Show
1313d ago - "KJ Reviews The Simpsons Arcade, Samurai Sword Destiny and Sakura Samurai. Are these titles worth... | Xbox 360
NWR: Samurai Sword Destiny Review
1350d ago - NWR: In UFO Interactive's Samurai Sword Destiny, you kill as many dudes as you can to gain money... | 3DS
Diehard GameFAN: Samurai Sword Destiny Review
1363d ago - DHGF: Samurai Sword Destiny tries to make the trek from the iOS platform to the 3DS eShop with mi... | 3DS
Samurai Sword Destiny (3DS eShop) Review - Nintendo Life
1364d ago - Nintendo Life's Jon Wahlgren says: "There's been a lot of ballyhoo about the death of the dedi... | 3DS
Tech-Gaming | Samurai Sword Destiny Review
1366d ago - As long as players are fundamentally opposed to a bit of repetition, Samurai Sword Destiny narrow... | Mobile
GEV: Nintendo 3DS Review: Samurai Sword Destiny
1366d ago - For those who have played Muramasa, this game will immediately feel familiar. I would like to pra... | 3DS
GameSpot: Samurai Sword Destiny Launch Trailer (3DS)
1368d ago - GameSpot: "Samurai Sword Destiny is now available in the Nintendo e-Shop." | 3DS
Samurai Sword Destiny Review (Modojo)
1369d ago - Chris Buffa (Modojo): Samurai Sword Destiny is a 2D hack-an-slash adventure from UFO Interactive.... | 3DS
3DS eShop Helps Kill Time During Post Holiday Rush
1370d ago - Chris Buffa (Modojo): It took a while, but we managed to survive the holiday gaming season, parti... | 3DS
IGN: Samurai Sword Destiny Review
1370d ago - Samurai Sword Destiny is trying to be Muramasa. Someone on this game's development team must have... | 3DS
13 stories found


text:
A Directory of federal R&D agencies' science and engineering programs for women, minorities, and physically handicapped persons
National Science Foundation, 1985 - Social Science - 27 pages
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1985 AWARDS NUMBER 20205 CONTACT PERSON 20550 CONTACT PERSON academic Aeronautics and Space Agricultural areas assist biological Biomedical Research Support biomedical sciences Blood Institute Building 31 cancer clinical DEADLINE FOR APPLICATION Department of Education Director TELEPHONE NUMBER diseases Education NAME ELIGIBILITY REQUIREMENTS eligible to apply Faculty Fellowships faculty members funded FY 1985 AWARDS graduate students Health NAME Health Professions Scholarships higher education Honors Undergraduate Research Indian Health Service Institutes of Health institutions of higher Lung MARC Mental Health Minority Biomedical Research Minority Institutions minority students N.W. Room NAME OF AGENCY NAME OF INSTITUTE NAME OF PROGRAM National Aeronautics National Institutes National Science Foundation NHLBI NIMH NUMBER AND SIZE NUMBER OF FY Ph.D physically handicapped Program ADDRESS program aims program is open program provides Project Grants Research Resources Room 1225 Washington S.W. Washington science and engineering Science Foundation NAME scientists stipend U.S. Department Westwood Building women