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Jeanne Jennings | August 6, 2012 | Comments
Learn how to create engaging content for your email newsletter, how to identify quality content, and get creative with content types and sourcing options (with bonus printout spreadsheet).
I am a big fan of well-done email newsletters. Why? Because they are one of the best ways to build relationships via email and boost your email return on investment (ROI).
As I've mentioned in past columns, editorial email messages (read: email newsletters) generate very high open and click-through rates compared to other types of email messages. Done properly, with a mix of no more than 40 percent marketing content and at least 60 percent editorial content, they can be a huge driver of conversions and revenue.
The biggest challenge that I see my clients face is sourcing quality editorial content for email newsletters. Here is an exercise I use with my clients (there's a spreadsheet for you to print below); it's simple and it will help you create engaging content for your email newsletter.
First, let's talk about how to identify quality content. You're looking for information that:
A great piece of quality content meets all four of these criteria; if it doesn't meet these four criteria, you should work with it until it does or not use it. A quick word on the last item, "supports your business goals." This doesn't mean that it should be promotional, just that it is in line with what your end goal is. A business goal could be showcasing your knowledge of the needs of the industry or other things that build relationships with your prospects and clients.
Next, you need to get creative with content types and sourcing options; I think of this as a mix-and-match proposition. Here's a short list of options:
|Types of Content
|Sources of Content
Notice the many different types of content, or content presentations, I've listed. Making your email newsletter more than just a bunch of articles helps with engagement. There is also some content that lends itself better to certain presentations than to others. Be sure you include both passive and interactive content in your newsletter.
Also, don't think that every item has to be 500 words or more. I like to mix in quick quotes and statistics to add interest. Only the quote or the stat actually appears in the newsletter, although I always include attribution with a link back to the original source for people that want to learn more. This is a great way to repurpose third-party content in a limited way.
Where to get the content is another conundrum many of my clients face. While it's always nice to create content from scratch, don't overlook the value of repurposing.
If you've got a presence on Facebook, LinkedIn, or another social network, or if you have one or more corporate blogs, there's a huge repurposing opportunity here. Pulling in interesting posts or, even better, comments, is an easy way to get content for your newsletter and drive people back there to contribute to the discussion. You can do the same with third-party blogs, but I love using social to drive email subscriptions and then using email to drive people to engage with you socially.
OK, here's where the exercise comes in.
You'll want to print the image below, or recreate it. You'll need to set aside some time (30 minutes to an hour) to focus on this exercise without any distractions.
The easiest way to begin is to think in terms of content you can repurpose. From there, let your imagination wander to what you could create from scratch. You should be familiar with what's available to be repurposed from your own company and the broader industry, but if you're not it's OK to be vague and go back to the Internet later to get more specific.
Your goal should be to have at least one item for each content type - even better, to fill the page with 24 content item ideas (they don't all have to appear in the same issue of your newsletter), which will give you a good starting point to develop your next editorial calendar.
Give the exercise a try and let me know how it goes!
Until next time,
Jeanne Jennings is a recognized expert in the email marketing industry and managing director of digital marketing for Digital Prism Advisors. She has more than 20 years of experience in the email and online marketing and product development world. Jeanne's direct-response approach to digital strategy, tactics, and creative direction helps organizations make their online marketing initiatives more effective and more profitable. Digital Prism Advisors helps established businesses unlock significant growth and revenue opportunities in the digital marketplace; our clients learn to develop and implement successful digital strategies, leveraging data and technology to better meet bottom line goals. Want to learn more? Check out Jeanne's blog and Digital Prism Advisors.
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An oversized item surcharge may apply to some heavy or bulky items due to size, weight or special handling required. This item surcharge is in addition to standard shipping rates and not included in shipping promotions.
Bath towels that can add a decorative touch to any bathroom. This bath towel features an embroidered hibiscus design & stripe border. Bath towel is 25 x 50.
Web Id: P000219135

subset: commoncrawl


text:
|By PR Newswire
Article Rating:
|April 7, 2014 09:09 PM EDT
LA JOLLA, Calif., April 7, 2014 /PRNewswire/ -- AV1 Group, Inc. (OTC Pink: AVOP) is pleased to announce that the Company's management has approved a friendly takeover by the Marijuana Incubator Group, Inc., an early contender in the rapidly evolving newly legalized Cannabis industry, in the interest of its shareholders and growth potential.
Marijuana Incubator Group, Inc. is an ambitious, now publicly traded, Company which is tapping into the groundbreaking Marijuana marketplace by seeking to develop the Company through Merger and Acquisition, and Joint Venture partnerships with established Cannabis related businesses such as harvesting operations and dispensaries, crop enhancement technologies for increased yields and revenue producing ecommerce industry related projects.
Currently cannabis is legal medicinally in 20 states including Colorado and Washington, but with the national elections impending, legalized territories are highly expected to increase. As momentum develops closer to a nationwide acceptance, the Company has set its sights on expansion into the California and Colorado markets, which are among the current forerunners in Marijuana legalization.
"We are pleased to be an early competitor in this robust evolving new industry; established and in operation as a public entity," stated Bryen Beglinger, Chief Executive Officer of Marijuana Incubator Group, Inc. He continued, "The opportunities currently available for a public company in this swiftly growing market have the potential to yield exponential growth, realizing a significant value for our shareholders."
The Company will be updating shareholders very soon regarding the direction of the Company and anticipated milestones.
About Marijuana Incubator Group, Inc.: Marijuana Incubator Group, Inc., also known as MIG, seeks to identify, secure and monetize emerging marijuana related companies that are positioned for exponential growth. We seek out talented entrepreneurs and potent ideas that are likely to make a major footprint in this sector. Our comprehensive business model allows us to foster these companies under one umbrella bringing a variety of experience, knowledge and expertise to every venture. MIG explores every opportunity to help our clients exceed their revenue goals while building close, active working relationships with our portfolio companies. For more information visit: www.MJIGINC.com
Safe Harbor Act: Forward-Looking Statements are included within the meaning of Section 27A of the Securities Act of 1933, and Section 21E of the Securities Exchange Act of 1934, as amended. All statements regarding our expected future financial position, results of operations, cash flows, financing plans, business strategy, products and services, competitive positions, growth opportunities, plans and objectives of management for future operations, including words such as "anticipate," "if," "believe," "plan," "estimate," "expect," "intend," "may," "could," "should," "will," and other similar expressions are forward-looking statements and involve risks, uncertainties and contingencies, many of which are beyond our control, which may cause actual results, performance, or achievements to differ materially from anticipated results, performance, or achievements. We are under no obligation to (and expressly disclaim any such obligation to) update or alter our forward-looking statements, whether as a result of new information, future events or otherwise.
Logo - http://photos.prnewswire.com/prnh/20140407/LA00334LOGO
SOURCE AV1 Group, Inc.; Marijuana Incubator Group, Inc.
Containers are revolutionizing the way we deploy and maintain our infrastructures, but monitoring and troubleshooting in a containerized environment can still be painful and impractical. Understanding even basic resource usage is difficult - let alone tracking network connections or malicious activity. In his session at DevOps Summit, Gianluca Borello, Sr. Software Engineer at Sysdig, will cover the current state of the art for container monitoring and visibility, including pros / cons and li...
Oct. 10, 2015 12:00 AM EDT Reads: 244
Too often with compelling new technologies market participants become overly enamored with that attractiveness of the technology and neglect underlying business drivers. This tendency, what some call the "newest shiny object syndrome," is understandable given that virtually all of us are heavily engaged in technology. But it is also mistaken. Without concrete business cases driving its deployment, IoT, like many other technologies before it, will fade into obscurity.
Oct. 10, 2015 12:00 AM EDT Reads: 171
Containers have changed the mind of IT in DevOps. They enable developers to work with dev, test, stage and production environments identically. Containers provide the right abstraction for microservices and many cloud platforms have integrated them into deployment pipelines. DevOps and Containers together help companies to achieve their business goals faster and more effectively.
Oct. 10, 2015 12:00 AM EDT Reads: 211
As a CIO, are your direct reports IT managers or are they IT leaders? The hard truth is that many IT managers have risen through the ranks based on their technical skills, not their leadership ability. Many are unable to effectively engage and inspire, creating forward momentum in the direction of desired change. Renowned for its approach to leadership and emphasis on their people, organizations increasingly look to our military for insight into these challenges.
Oct. 9, 2015 11:15 PM EDT Reads: 198
SYS-CON Events announced today that Dyn, the worldwide leader in Internet Performance, will exhibit at SYS-CON's 17th International Cloud Expo®, which will take place on November 3-5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. Dyn is a cloud-based Internet Performance company. Dyn helps companies monitor, control, and optimize online infrastructure for an exceptional end-user experience. Through a world-class network and unrivaled, objective intelligence into Internet condit...
Oct. 9, 2015 11:00 PM EDT Reads: 644
Today air travel is a minefield of delays, hassles and customer disappointment. Airlines struggle to revitalize the experience. GE and M2Mi will demonstrate practical examples of how IoT solutions are helping airlines bring back personalization, reduce trip time and improve reliability. In their session at @ThingsExpo, Shyam Varan Nath, Principal Architect with GE, and Dr. Sarah Cooper, M2Mi's VP Business Development and Engineering, will explore the IoT cloud-based platform technologies driv...
Oct. 9, 2015 10:15 PM EDT Reads: 137
The IoT market is on track to hit $7.1 trillion in 2020. The reality is that only a handful of companies are ready for this massive demand. There are a lot of barriers, paint points, traps, and hidden roadblocks. How can we deal with these issues and challenges? The paradigm has changed. Old-style ad-hoc trial-and-error ways will certainly lead you to the dead end. What is mandatory is an overarching and adaptive approach to effectively handle the rapid changes and exponential growth.
Oct. 9, 2015 10:00 PM EDT Reads: 244
Who are you? How do you introduce yourself? Do you use a name, or do you greet a friend by the last four digits of his social security number? Assuming you don't, why are we content to associate our identity with 10 random digits assigned by our phone company? Identity is an issue that affects everyone, but as individuals we don't spend a lot of time thinking about it. In his session at @ThingsExpo, Ben Klang, Founder & President of Mojo Lingo, will discuss the impact of technology on identity....
Oct. 9, 2015 10:00 PM EDT Reads: 453
SYS-CON Events announced today that Spirent Communications, the leader in testing navigation and positioning systems, will exhibit at SYS-CON's @DevOpsSummit Silicon Valley, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. Spirent Communications enables innovations in communications technologies that help connect people. Whether it is service provider, data centers, enterprise IT networks, mobile communications, connected vehicles or the Inte...
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There are many considerations when moving applications from on-premise to cloud. It is critical to understand the benefits and also challenges of this migration. A successful migration will result in lower Total Cost of Ownership, yet offer the same or higher level of robustness. Migration to cloud shifts computing resources from your data center, which can yield significant advantages provided that the cloud vendor an offer enterprise-grade quality for your application.
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DevOps is gaining traction in the federal government – and for good reasons. Heightened user expectations are pushing IT organizations to accelerate application development and support more innovation. At the same time, budgetary constraints require that agencies find ways to decrease the cost of developing, maintaining, and running applications. IT now faces a daunting task: do more and react faster than ever before – all with fewer resources.
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The web app is agile. The REST API is agile. The testing and planning are agile. But alas, data infrastructures certainly are not. Once an application matures, changing the shape or indexing scheme of data often forces at best a top down planning exercise and at worst includes schema changes that force downtime. The time has come for a new approach that fundamentally advances the agility of distributed data infrastructures. Come learn about a new solution to the problems faced by software organ...
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The buzz continues for cloud, data analytics and the Internet of Things (IoT) and their collective impact across all industries. But a new conversation is emerging - how do companies use industry disruption and technology enablers to lead in markets undergoing change, uncertainty and ambiguity? Organizations of all sizes need to evolve and transform, often under massive pressure, as industry lines blur and merge and traditional business models are assaulted and turned upside down. In this new da...
Oct. 9, 2015 08:00 PM EDT Reads: 316
Achim Weiss is Chief Executive Officer and co-founder of ProfitBricks. In 1995, he broke off his studies to co-found the web hosting company "Schlund+Partner." The company "Schlund+Partner" later became the 1&1 web hosting product line. From 1995 to 2008, he was the technical director for several important projects: the largest web hosting platform in the world, the second largest DSL platform, a video on-demand delivery network, the largest eMail backend in Europe, and a universal billing syste...
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Electric power utilities face relentless pressure on their financial performance, and reducing distribution grid losses is one of the last untapped opportunities to meet their business goals. Combining IoT-enabled sensors and cloud-based data analytics, utilities now are able to find, quantify and reduce losses faster – and with a smaller IT footprint. Solutions exist using Internet-enabled sensors deployed temporarily at strategic locations within the distribution grid to measure actual line lo...
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Sapphire Radeon HD 6870 1GB Vapor-X Video Card Review - Final Thoughts
Final Thoughts
The Sapphire HD 6870 Vapor-X didn't shock us. We've come to expect the Vapor-X series from Sapphire to deliver, and again it does with the HD 6870 getting the treatment in this case.
Performance doesn't hold any surprises with Sapphire choosing to just use the reference clocks in these situations, but you can always overclock yourself. The higher quality cooler should also bring with it a little more headroom thanks to the temperature being a little lower at stock speeds.
What is great to see from the model, though, is the cables included in the box. It's nice to see that Sapphire hasn't just added the mini-DP to DP convertor and HDMI cable to its higher end HD 6900 series, but has also put it in this HD 6870. There's nothing worse than getting the card home to find you can't make use of that sexy DisplayPort connection, and instead have to order an adapter separately which will take time to come and also add more expense on the card.
We've looked at a couple of great HD 6950s in the last few days, but for some the card continues to be a little expensive. If you want to take it a step down, save some money but still get strong performance, the HD 6870 Vapor-X from Sapphire is a great buy at $229.99 over at Newegg.
With strong performance and excellent cooling, it's another hard AMD model to pass up if it's in the price range you're looking at.
PRICING: You can find products similar to this one for sale below.
United States: Find other tech and computer products like this over at Amazon's website.
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Hampton Inn - Warner Robins
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The Lake in Georgia and Fried Green Tomatoes
... like to say look a bit inbred (who said that????) But the lady in the shop was very helpful and told us about a long lost friend who was British and had moved to America back in the day, she had an invite to Diana and Charles wedding but she couldn't make the wedding due to money so she sent Diana a present to say congratulations which was a tea set and Diana personally wrote back to the USA to say thank you and how unfortunate it was she couldn't make the wedding. (How cool is that) ...
Big traffic jams and high humidity
A quick blog today. I will add to when I have more time. At this stage all I will say is that we are late in due to horrendous traffic on various interstates. It has been very hot and humid so need a drink and some food. I will catch up with you ...
On the road again...
... speechless. LOL
I haven't seen mom for a couple of years so it was too bad that we had such a limited time to visit. We did stay for about an hour. Most of that time I worked on her computer (yes, my mother is 88 and does email!) while she and Jeff chatted.
Eventually, we had to say goodbye and get back in the car.
This day is going to be a little different than you may have thought (surely you aren't surprised!). Last night Jeff figured out that ...
Deep Cultural Southern Roots
... National Historic Site, Atlanta, Georgia. This historic site is centered around Martin Luther King, Jr's boyhood home near downtown Atlanta and also includes Ebenezer Baptist Church, which is where his father, and later Martin Luther King Jr, himself preached. We were able to take a tour of his home, but no photos were allowed inside. We also took a self guided tour of the church. One could almost hear his sermons that came from the pulpit. ...
A Thankful Thanksgiving
... thrown into the fray of crazy people trying to grab insignificant items for a few dollars. Just the combination of the people and intense atmosphere made me want to punch the nearest person in the face for almost no reason. I have been assured by Ible that our shopping experience should not be like that. So, I am looking forward to tomorrow morning and whatever it may bring.
On the 29th, after the ...


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Illinois Public Media
All articles tagged Media Matters
Former health insurance industry executive Wendell Potter
Danny Schechter, founder of MediaChannel.org
Mike Knezovich, Jenny Southlynn and Carl Estabrook
Stephen Zunes, professor of politics
Francis Boyle, professor
Barbara Ehrenreich, author
Carrie McLaren and Gary Ruskin
Sut Jhally, Professor
Page 1 of 1 pages
WILL Highlights
Vintage Vinyl is coming!
The Vintage Vinyl Sale to benefit Illinois Radio Reader returns on October 3. Click for the latest info.
WILL News wins PRNDI Award for "Unmet Needs" Radio Doc
The 2014 radio documentary, produced by former WILL staff and hosted by Scott Cameron, won second place in the Long Form Documentary category among medium-sized stations.
WILL Ag Director Dave Dickey retires
He carried on the farm broadcasting tradition, while innovating on the Internet.


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Airborne Vaginal Discharge. The squirting of ones vagina after rigorous ciltoral stimulation. The equivalent of a male ejaculation.
Dude that girl I took home last night was an AVDer! Just a fair warning I AVD! Did you hear about Ashley`s AVD last night?
by Big Bobby k December 06, 2009
A filthy smutty person.
Sis you shat on my pizza AVD.
by Henry The Tortoise December 11, 2008


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[META] A Slight Change to Title Formatting (2015) (self.NetflixBestOf)
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[META] 3rd Annual NetFlixBestOf 31 Days of Horror MEGALIST! (self.NetflixBestOf)
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[US] Dark Matter (2015) - 13 episode season. 6 very different people wake up on a space ship with no memory of who they are or where the ship is going. A mashup of Firefly, Pandorum, and Persons Unknown. Not groundbreaking, but thoroughly enjoyable binge watch. (netflix.com)
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[US] The French Connection (1971): "Popeye" Doyle (Gene Hackman) and his partner (Roy Scheider) are New York City police detectives on narcotics detail, trying to track down the source of heroin from Europe into the United States. Oscar winner for Best Picture, Best Director, Best Actor, 98% RT. (netflix.com)
submitted by chodegarglerLOL
[US] Outside Providence (1999): "Dildo" Dunphy gets into some trouble and ends up out of his element after being sent to a prep school in this comedy set in the '70s. Alec Baldwin is brilliant in his role as Dunphy's dad. Based on the novel by Peter Farrelly (of the Farrelly brothers.) (netflix.com)
submitted by MimsyMayhem
[Request] [US] A modern classic 80s/90s movie (self.NetflixBestOf)
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[REQUEST] Movies with a Tom Waits aesthetic. (self.NetflixBestOf)
submitted by MasterThomasPopkiss
[REQUEST] [UK] Box office bombs (self.NetflixBestOf)
submitted by chinberg
[REQUEST] Best movies for a dark and stormy night? (self.NetflixBestOf)
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Apps: Google Maps 3.0, Intake, Republique 2.0 + Tumblr 3.6
Google has updated Google Maps (free) to version 3.0.0. The major update includes many new features, including lane guidance within turn-by-turn navigation. Offline maps can now be saved for use when traveling or when dealing with a slow connection. The app also works in conjunction with Uber — users with the Uber app can open it straight from Google Maps. Users can now view travel time, distance, and estimated arrival at the same time in navigation mode, and transit results display total walking time and the next scheduled arrival of a bus or train. Additional new features include filtering business search results by rating, price, and opening hours, searching using Google Voice Search, and more.
Cipher Prime Studios has released some of the most visually appealing action-puzzle games we've seen for the iPad — Pulse, Splice, and Fractal among them, all solely for Apple's tablets rather than pocket devices. Intake: Be Aggressive ($3) is its latest title, and a very interesting, retro-influenced experience, even though it's not the company's best effort in the gameplay department. The gameplay is extremely simple: two colors of pills stream from the top of the screen to the bottom, and you need to tap every pill of at least one of the colors to keep playing; a glow at the bottom of the screen shows you which pill color will be absorbed by a shield if they're missed, rather than instantly ending the game with a fatal dosage. You can tap every pill if you want, but you get combo bonuses for focusing on a single color pill over and over; you'll only advance to the next level if you repeatedly hit the right color. While the gameplay is changed up a little by the eventual addition of neutrally-colored pills and unlockable powerups, what will hook you or not are the glowing special effects, the cool electronic soundtrack, and the increasing pace at which the pills drop over time. Intake's goal is to hook you with small but thoughtful aesthetic and gameplay elements; if you're intrigued by this description, you'll likely be pleased by the game.
Camouflaj's Republique, an action-adventure game that first hit the App Store last year, recently released episode two in version 2.0. Republique is a stealth-action game — a genre that's seen a lot of good entries into the App Store recently—controlled through taps and switching between different cameras as the main character is monitored from afar. But Republique is clearly more of an investment, as the game's developers describe the title as "one of the most ambitious games ever developed for iOS." Episode two is titled Metamorphosis, and includes new puzzles, a new environment, a new guard type, and a 3D map feature that works in both the first and second episodes. The game costs $5 — though it's currently on sale for $3 — and the new episode will set you back another $5. A season pass for all future episodes costs $15.
The newest update to Tumblr (free) gives you control of your blog's look directly from the app. Prior to version 3.6, a Tumblr's appearance could only be edited on the web. Now, users can change backgrounds, colors, fonts, and pictures all from the iOS app. When paired with the already existing ability to post updates from the app, Tumblr users now have even less reason to visit the web-based version of the site.
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"Bringing The Great American Song Book into the 21st century"
Hey friends & fans,
Thanks for visiting my site. Browse around, listen to my music, check out the photo's and video's page, see where I'm playing, Buy a CD. Hope to see you at one of my shows, or on the bandstand.
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Dedicated to the preservation of "The Great American Song Book" Jerry Costanzo is considered one of the best and busiest singer/bandleaders on the scene today. Jerry has gained popularity among connoisseurs of this great music. He appeals to audiences young and old, his interpretations of timeless "Standards" from the song writing legends will set the stage and carry listeners back to an era when this sultry and swinging music was the "Pop" of the day.
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An antisense transcript of histone H2a that has no significant protein-coding region has been cloned from a mouse full-length cDNA library. In the present study, we evaluated this transcript by using RT-PCR and compared the expression patterns of the sense and antisense transcripts by using quantitative RT-PCR (qRT-PCR).
This antisense RNA was expressed in three mouse cell lines. We call it ASH2a. ASH2a includes not only the complementary sequence of the transcript of Hist2h2aa2 (a replication-dependent histone H2a gene), but also that of the promoter of Hist2h2aa2. The upstream genomic sequence of the transcription start site of the ASH2a-coding gene (ASH2a) lacks both CCAAT and TATA boxes. This absence suggests that the regulation of ASH2a is different from that of the replication-dependent histone H2a genes. Findings from qRT-PCR indicated that the expression pattern of ASH2a was different from that of Hist2h2aa2. Expression of Hist2h2aa2 peaked at 2 to 4 h during S-phase, but that of ASH2a peaked at 1 h.
We showed the existence of ASH2a, a histone H2a antisense RNA, in mouse cultured cells. The expression pattern of ASH2a is different from that of the sense RNA.
A comprehensive search of the Functional Annotation of Mouse (FANTOM) database revealed about 30000 full-length cDNA clones without a significant protein-coding region [1]. Indeed, antisense transcripts seem to be present in 10% to 20% of genes in human and mouse genomes [2-6]. These findings suggest that many biological reactions related to antisense transcripts and/or protein-noncoding transcripts are still unrevealed [7]. On the other hand, the cDNA database sometimes includes reverse complements of real transcripts. These artifacts are excluded from the database on the basis of the sequences of intron-splicing sites. Therefore, transcripts without any introns need more experimental evaluation than computational annotation to ascertain their validity.
Histone mRNAs regulated by the cell cycle increase at the beginning of S-phase and decrease at the end of S-phase [8]. Among 20 histone H2a-coding genes, 18 are replication-dependent; the other 2 are replication-independent [9]. The replication-dependent genes lack introns and a poly (A) signal and contain a highly conserved stem-loop structure at the 3' end of the mRNA. This stem-loop structure plays an important role in mRNA processing and stability [10-12]. The promoters of the replication-dependent histone genes contain CCAAT and TATA boxes [13].
As far as we know, Drosophila histone H3 antisense [14] and Leishmania histone H1 antisense [15] transcripts have been reported, but no histone H2a antisense RNA or mammalian histone antisense RNA has been reported. FANTOM 2 [1] contains an antisense transcript (FANTOM clone ID 2210403F13; accession number AK028129) of histone H2a. We call this antisense transcript ASH2a. Comparison of the nucleotide sequence of ASH2a and the mouse genome sequence showed that the ASH2a-coding gene (ASH2a) lies on chromosome 6 without introns. The sequence of ASH2a is exactly complementary to that of the coding region of Hist2h2aa2 (a replication-dependent histone H2a gene) and that of the promoter. In the present study, we evaluated ASH2a transcript by using RT-PCR and compared the expression patterns of Hist2h2aa2 and ASH2a by using qRT-PCR.
Detection of ASH2a
First, cDNAs were synthesized by using the random hexamer oligonucleotide for RNAs from the Hepa 1–6, 3T3, and LLC cell lines. RT-PCR was performed for two targets, the sense-antisense overlap region (between F1 and R1 in Fig. 1) and the overlap region plus the antisense-unique region (between F1 and R2 in Fig. 1). All PCR products for both targets had expected sizes (Fig. 2a). To check the PCR products, we digested them with PstI (Fig. 2b). All digests of the PCR products for the region had the expected sizes (251 and 267 bp for the sense-antisense overlap regions; 251 and 858 bp for the overlap region plus the antisense-unique regions).
Figure 1. (a) Nucleotide sequence of ASH2a. ASH2a is encoded from positions 1 to 2427. Bold characters indicate overlap with the Hist2h2aa2 transcript (italic = protein-coding region). Arrows indicate primers used in this study. (b) Relationship between Hist2h2aa2 and ASH2a RNAs. Arrows indicate the locations of the primers.
Figure 2. (a) RT-PCR products from cDNAs obtained by priming total RNA with random hexamers. Lanes: 1, DNA ladder (100-bp ladder, TOYOBO); 2–7, RT-PCR products amplified with primers F1 and R1 (upper) and those amplified with primers F1 and R2 (lower). RNA was extracted from Hepa 1–6 (lanes 2 and 5), 3T3 (lanes 3 and 6), and LLC (lanes 4 and 7) cells. Superscript III was not added in the reaction of lanes 2–4. Lane 8, PCR product of genomic DNA amplified with primers F1 and R1 (upper) and that amplified with primers F1 and R2 (lower). Arrows indicate the expected products. (b) Patterns of digestion of PCR products by PstI. Lanes: 1, DNA ladder; 2–4, digests of PCR products amplified with primers F1 and R1. PCR product was produced from Hepa 1–6 (lane 2), 3T3 (lane 3), and LLC (lane 4). Lanes 5–7, digests of PCR products amplified with primers F1 and R2. PCR product was produced from Hepa 1–6 (lane 5), 3T3 (lane 6), and LLC (lane 7). Arrows indicate the expected products. (c) RT-PCR products and the EcoRI-digest patterns of cDNAs obtained by priming total RNA with the specific primer R3. Lanes: 1 and 8, DNA ladder; 2–7, RT-PCR products amplified with primers F2 and R3. RNA was extracted from Hepa 1–6 (lanes 2 and 5), 3T3 (lanes 3 and 6), and LLC (lanes 4 and 7). Superscript III was not added in the reaction of lanes 2–4. Lanes 9–11, EcoRI-digests of PCR products amplified with primers F2 and R3. PCR product was produced from Hepa 1–6 (lane 9), 3T3 (lane 10), and LLC (lane 11). Arrows indicate the expected products.
Second, to elucidate the expression of ASH2a, we transcribed the first-strand cDNA with primer R3, which hybridizes specifically to ASH2a. A product of the expected size (685 bp) was obtained (Fig. 2c). In addition, EcoRI digested the PCR product to 269- and 416-bp fragments (Fig. 2c). These results are consistent with the nucleotide sequence of ASH2a.
Quantitative RT-PCR
Because the sequence of the Hist2h2aa2 transcript is not unique, being completely overlapped by the ASH2a sequence, the expression level detected by qRT-PCR using random primers is the sum of the Hist2h2aa2 and ASH2a levels (Fig. 1). Because ASH2a has a unique sequence, the expression level of this antisense transcript can be detected separately. Observation using qRT-PCR indicated that the sum of the Hist2h2aa2 and ASH2a expression levels was always much higher than the level of ASH2a (Fig. 3). The difference between both CT values is more than 4. Thus, we estimated the expression of Hist2h2aa2 and ASH2a as that of Hist2h2aa2 in the following study. Along with cell cycle progression from S-phase, the expression of Hist2h2aa2 increased and peaked at 2 to 4 h, and then decreased (Fig. 4). After that, it increased again, but the expression level was lower than the S-phase peak. Thus, the expression is the highest in the middle of S-phase. On the other hand, that of ASH2a peaked at 1 h, and fell to basal level thereafter (Fig. 4). The rate of the increase of ASH2a RNA was lower than that of Hist2h2aa2 RNA.
Figure 3. Representative amplification plot. Curves indicate amplification from transcripts of GAPDH (first group of rising curves), Hist2h2aa2 (second), and ASH2a (third). Different colors indicate that each result from 0 h to 12 h (13 points). X-axis, cycle numbers; Y-axis, ΔRn.
Figure 4. Transcript expression patterns. (a) Transcripts of Hist2h2aa2. (b) Transcripts of ASH2a. X-axis, time (hours); Y-axis, relative expression level, adjusted to 1.0 at 0 h. The qRT-PCR analyses were performed 4 times (indicated by different colors).
An antisense transcript of histone H2a (ASH2a) was clearly expressed in three mouse cell lines, Hepa 1–6, 3T3, and LLC. This result strongly suggests that ASH2a is not regulated with tissue specificity. We checked the upstream regions of ASH2a and the promoter regions of mouse histone H2a genes. All promoters of replication-dependent histone H2a genes include CCAAT and TATA boxes [13]. On the other hand, the upstream region of ASH2a lacks such a structure (Fig. 1). This region has a G+C-rich sequence but lacks both CCAAT and TATA boxes (Fig. 1). Therefore, the regulation of ASH2a is strongly suggested to be different from those of replication-dependent histone H2a genes.
To check the synchronization of the cells, we compared the expression patterns of the replication-dependent histone gene Hist2h2aa2 and the replication-independent histone gene H2afz [16]. Hepa 1–6 cells used in the present study were well synchronized. In fact, the expression pattern of ASH2a was different from that of Hist2h2aa2. The amount of sense RNA was always much higher than that of ASH2a RNA at each time point. Three general functions of antisense transcripts have been identified: transcriptional interference, RNA masking, and dsRNA-dependent mechanisms, including RNA interference [17]. These functions are related to inhibition and/or degradation of sense RNAs. If ASH2a is related to the degradation of the sense RNA through dsRNA formation, ASH2a would be expressed when the sense RNA decreases. However, ASH2a is expressed during the early increase of the sense RNA.
On the other hand, ASH2a could hybridize not only to the Hist2h2aa2 transcript, but also to the transcripts of the other histone H2a-coding genes, because of the high similarity of protein coding sequences. A recent article showed that a small modulatory dsRNA can function as an activator of related genes [18], and the mechanism of action appears to be mediated through a dsRNA/protein interaction, rather than through siRNA or miRNA. Interestingly, ASH2a includes not only a sequence complementary to that of the Hist2h2aa2 transcript, but also a sequence complementary to the promoter region of Hist2h2aa2. Additional work is needed to elucidate the function of the ASH2a-related dsRNA.
Experiments using the high-density oligonucleotide arrays show that a large population of noncoding RNAs are expressed and regulated by similar molecular mechanisms to those involved in the control of protein-coding RNAs [19] and that many transcripts appear to be at very low abundance [20]. It is so important for genome research to elucidate the functions and regulation of noncoding RNAs and antisense RNAs at very low abundance such as ASH2a.
Materials and Methods
Cell lines
Murine hepatoma cell line Hepa 1–6, fibroblast cell line Flp-In-3T3 (Invitrogen), and lung carcinoma cell line LLC were cultured in DMEM supplemented with 10% fetal calf serum.
Cell cycle synchronization
Hepa 1–6 cells were synchronized at the end of G1 phase by the addition of thymidine-hydroxyurea. The cell cycle arrest was released by washing out the thymidine-hydroxyurea, then the cells were harvested at intervals of 1 h from 0 h to 12 h.
Total RNA fractions extracted from mouse cells were pre-treated with DNase I and used for RT-PCR. Reaction mixture containing the RNA (approximately 0.5 μg) and the strand-specific primer (3.3 pmol) or random hexamer primers was denatured at 70°C, and then reverse-transcription reaction was done with Superscript III (Invitrogen) according to the manual. Then the cDNA was amplified by PCR under the condition of 35 or 40 cycles of 30 s at 95°C, 30 s at 54°C, and 1 or 2 min at 72°C. The sequences of primers are shown in Fig. 1. For quantitative RT-PCR (qRT-PCR), approximately 12.5 ng of total RNA was used for reverse transcription followed by PCR amplification with primers qF1 and R1, or qF2 and R2 (Fig. 1) in reaction mixture containing SYBR premix Ex Taq (Takara) in an ABI PRISM 7700 sequence detection system (Applied Biosystems). The PCR conditions were an initial step of 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 30 s at 60°C. Expression was assessed by evaluating threshold cycle (CT) values. The relative amount of expressed RNA was calculated using Livak and Schmittgen's method [21]. Quantification of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA (primers 5'-TGTGTCCGTCGTGGATCTGA-3' and 5'-CCTGCTTCACCACCTTCTTGA-3'; product size 76 bp) was used as a control for data normalization. The qRT-PCR analyses were performed four times.
Authors' contributions
HN designed this study, carried out the molecular biological studies. YT carried out synchronization of cells and quantitative PCR. YO and YH participated in the design of this study.
We thank the anonymous reviewer for their helpful comments. We are grateful to Yap Chan Choo, Misato Nakanishi, Taku Tanaka, and Masanori Suzuki for helpful comments. This work was supported in part by a Research Grant for the RIKEN Genome Exploration Research Project, a Research Grant for Advanced and Innovational Research Program in Life Science, and a Research Grant for the Genome Network Project from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of the Japanese Government, and in part by a Research Grant for CREST of Japan Science and Technology Corporation to Y.H., and supported in part by grant 15770055 from the MEXT to H.N.
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www.pnbindia.in Recruitment 2015
Punjab National Bank has expanded an online advertisement regarding the www.pnbindia.in Recruitment 2015 for filling up 09 posts of Male Hockey players in Clerical Cadre. Punjab National Bank is looking for dedicated and talented contenders to fill these posts.
Contenders who have required eligibility and desire to make their career in banking sector can apply for PNB job opportunities by submitting PNB application form on or earlier than last date.
Quick Details of www.pnbindia.in Recruitment 2015
Name of Organization
Punjab National Bank
Name of Posts
Hockey Player (Male only) In Clerical Cadre
No. of Posts
Qualification Required
10th pass
Age Limit
18 to 26 yrs
Selection process
Sports Performance/ Field Trials And Personal Interview
Closing date to apply
20th June 2015
Punjab National Bank is one of the best bank of India, which works with Million of satisfied customers. PNB has always looked at technology as a key factor to provide better services to its customers. The Bank has more than 3700 ATMS has the widest ATM network amongst nationalized Banks. Now PNB invites applications from Indian Citizens for 09 Posts of Male Hockey Players in the Clerical Cadre. Interested candidates must hold this chance and apply for PNB job openings till the last date.
Applicants who are willing to apply for PNB Jobs may acquire complete details concerning to www.pnbindia.in Recruitment 2015 such as eligibility criteria, selection procedure, salary How to apply etc which is tuned below by the squad of privatejobshub.
Educational Qualification:
Applicants who want to take the advantage of PNB Jobs should pass 10th standard or equivalent.
Sports Qualification:
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How to apply for www.pnbindia.in Recruitment 2015?
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- Applicants are required to submit filled application form through proper channel along with all required documents/ certificates on or before the closing date.
Important Date:
Last date for submission of application: 20th June 2015
Note: To get more information about www.pnbindia.in Recruitment 2016 you may go through Official Link.
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At Dillard's, we implemented a security program to ensure that a human is trying to enter our site and not a computer. If you have any questions, please contact firstname.lastname@example.com.
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Item #04163141
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(based on 15 reviews)
Ratings Distribution
5 Stars
4 Stars
3 Stars
2 Stars
1 Stars
of respondents would recommend this to a friend.
Best Uses
Feels full size too small
Feels half size too small
Feels true to size
83% (10 reviews)
(10 reviews)
Feels half size too big
Feels full size too big
Feels too narrow
Feels true to width
Feels too wide
Most Liked Positive Review
Comfortable & cute wedge sandals
I purchased this wedge sandal in luggage tan in my normal 6.5 size. It fits great and feels comfortable after wearing even for long periods of time. I usually have trouble with blisters and...Read complete review
I purchased this wedge sandal in luggage tan in my normal 6.5 size. It fits great and feels comfortable after wearing even for long periods of time. I usually have trouble with blisters and have wide feet. Antonio Melani makes a great shoe that fits my feet perfectly. I love this shoe so much, I plan to purchase them in the red as possibly black as well!
Most Liked Negative Review
Cute but not comfy
These shoes are adorable! Even cuter in person then online. However, the right shoe was so tight around the top! I have never had that issue with any other pair of shoes...Read complete review
These shoes are adorable! Even cuter in person then online. However, the right shoe was so tight around the top! I have never had that issue with any other pair of shoes (and I own a lot of shoes). I took them to the store and tried on a different pair, just to be sure, and again the right shoe was Too tight, so maybe they are just for narrow feet (my feet are not wide, just normal). My advice is try them on first or be prepared (unless you have narrow feet!)
Reviewed by 15 customers
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Super Cute
By LV Mom of 2
from Leavenworth, KS
See all my reviews
Comments about Antonio Melani Amirah Wedges:
I love the look of these shoes, they are super cute and very comfortable!
Bottom Line Yes, I would recommend this to a friend
Was this review helpful? Yes / No
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Comment on this review
love them
By Boater girl
from Rocky River, Ohio
This is my second pair of basically the same shoe from last summer season. I wore them out. Loved them with skirts, shorts, cropped pants and felt they were a nice balance in between too dressy and too casual. This season's version has the brand name across the top which I do to love. Last year's had an attractive design on top without the brand name. Love the shoe overall. It's comfortable, a nice heel height (better than flats- makes legs look longer). The mixed materials allows me to wear it with nearly everything.
By Carolinacuties
from SC
About Me Stylish
Not so comfortable
By Thea
from Miami, Fl
I thought these would be comfortable because of the low heel and the cushioned insole, but the strap that goes over the foot was too tight and the piece that goes between the toes, as well as the sole were too stiff, which hurt my feet with every step. I exchanged them for another Antonio Melani sandals that although are high platforms are much more comfortable.
Bottom Line No, I would not recommend this to a friend
Very cute
By Deborah
from FL
Delivery was incredibly fast. I will definitely use your on-line shopping service again.
By Jade
from Lake Charles, la
When I got these in the mail they were better than I thought they would be from the website. I love them!
Too Short
By Lanier
from Florida
Runs small
very comfortable
By Ammy
from Texas
I have two pairs because i like the slight wedge.
Pleased with new purchase
By Insurance rep
from Humble tx
About Me Conservative
Great sandals ,very comfortable, not too much shine !
By Josie
from Tucson, AZ
About Me Trendy
These are AMAZING! Finally sandals that are adorable and comfy!


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The Top 10 In Action Sports
01Kenton Cool And The Legend Of Everest
02The Martyn Ashton Interview: Meet the Man Who Got Back on the Bike
03Legends Behind the Lens: The Dan Milner Interview
04We Asked Surf Star Coco Ho For Her Step-by-Step Guide to the Basics
05The Tony Hawk Interview: How Is The Birdman Still Flying High After All These Years?
06We Toured Britain's Action Sports Hotspots With The New Ford S-MAX
07The Legend Of El Dorado: One Man's Obsession With Finding A City Made Of Gold
08I Am Legend: How Audiences – Real Or GoPro'ed – Can Affect Your Mind And Performance
09Watch A Freerunning King Defy The Laws Of Gravity In This Urban Assault On London
10Living On The Blood and Urine of Dead Sea Turtles: The Shocking Story of A Sailor Stuck in Hell
Marek Zaprazny and Nikolas Tusl in Barcelona - May2010
A Skateboarding video by martinkaras - 18,020 views
Nike SB and City Stars rider Marek Zaprazny went to Barcelona in May 2010 with his friend Nikolas Tusl from Hype Skateboards. This is video from their 5day stay.
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Julie Podest | Robert Wexelblatt
Choosing between right and wrong isn't hard. I mean you might still choose badly, but if you do, you know it's wrong. Like if you're at the pool and a baby crawls to the edge and slips into the water because her mother's busy [more…]
Genre: Short Story


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Archived Discussion
This is discussion archived from a time before the current discussion method was installed.
Jonny D: Anyone who ended up here from Temple O Traps, it looks like someone went ahead and copied the content from there to a new Temple of Doom without setting up a redirect. Since it looks like Temple Of Doom is now the main version of this article, I've set up a redirect. The discussion from Temple O Traps appears below:
- Temple O Traps DiscussionTanto: Ugh, this name sucks.SAMAS: You got a better one, then use it with my blessing. Please, please use it. ^_^HeartBurn Kid: Temple of Doom? After all, there's no denying that most games that use temple levels draw scads of inspiration from Indiana Jones...


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UAE: Biggest climber in visa index
Click here for the top 10 rankings in the regionOctober 8, 2015 6:09
Register for our free newsletter
MIDEAST STOCKS-Markets to rise on stronger global environment
Abu Dhabi's ADWEA in talks with banks for $350 mln project bond
Emirates hints at no Dubai Airshow orders, pushes decision on A350/787 to next year
UAE minister says plans to boost oil output capacity on track
MIDEAST STOCKS-Saudi, Egypt markets barely move in early trade
Qatar Q2 GDP growth accelerates to 4.8 pct year/year
UAE says reforms will better protect foreign workers
United Arab Bank secures $125 mln three-year loan
MIDEAST STOCKS-Saudi hit by weak oil, commodities; Egypt down modestly
MIDEAST STOCKS-UAE markets narrowly mixed in thin, early trade
Emirate of Ras al-Khaimah eyes benchmark bond issue-paper.
October 15, 2010 11:26 by Reuters
The government of Ras al-Khaimah, one of seven emirates making up the United Arab Emirates, is planning a bond issue worth at least $500 million, a newspaper reported on Friday, citing banking sources.
Royal Bank of Scotland and Citigroup were among the arrangers for the "benchmark-size" bond issue, the daily Gulf News cited banking sources as saying.
"The government could be targeting a larger deal," a banker involved in the deal told Gulf News.
A government official in Ras al-Khaimah, the UAE's most northern emirate, would not confirm plans for a bond issue but told Reuters the emirate was planning to reduce its debt towards the end of the year.
"Ras al Khaimah is looking at a number of mechanisms to do so," the official said, without giving further details.
Ras al-Khaimah has an A rating from Fitch and Standard & Poor's.
In May 2008, Ras al-Khaimah announced an Islamic bond programme worth up to $2 billion to fund infrastructure projects and launched the first, $272 million-tranche in the same month. Last year, the emirate issued another $400 million worth of sukuk.
The government of Dubai in September launched its first sovereign bond issue since a debt crisis that rattled investors a year ago, attracting healthy demand for the $1.25 billion on offer.
(Reporting by Raissa Kasolowsky; Editing by Susan Fenton)
Iran wants to discuss dates to start nuclear talks
October 15 2010
Zurich acquires Compagnie Libanaise D'Assurances
October 15 2010


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Swensen'sOctober 19, 2011American • Singapore
Try the beef baked rice and mango lychee...member pay only $1.00 for any drink!!!!
Try the beef baked rice and mango lychee...member pay only $1.00 for any drink!!!!
Must watch the uncle dance to beat of music while dishing up the deserts
Hoohoo 1 person than my turn...choose to see Regina instead of Sebastian (loooooonnnggg queue)
Dangerous lady driver in silver BMW car plate no. SJX....talking on mobile while coming out of carpark @ causeway point with children in the car!!!!
Always look for Esther, she's the best!!!!!
There's no Blk 810b in the whole of Sembawang!!!!!!!! Are you dreaming?!?!
Waited for more than 15mins before the staff came and took our order....bad service, doubt I'll ever come again
The uncle selling dessert dance to the beat of the music.....
There's a queue sign but when u stand in the queue nobody wants to take yr order!!!! so proud!!!!
This idiot with his family occupying subway seats bit eating MOS burger such a good model for his children!!!!
Got my willows for CNY at $7 plus staff helps to cut to required length..brilliant!!!
Never visit at 9pm esp on sun/min night. Long queue. Friendly staff


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Employment Law Issues for Health Care Employers
OFCCPs New Veteran/Disability Regulations Are Now in Effect. Are You Ready?
Should an employer have a written social media policy?
Employer Social Media Policies – Interview with Mitch Danzig, Member, Member, Mintz Levin
Two Key Elements Every Social Media Policy Should Include
Corporate Law Report: Global HR, Textual Harassment, Working Interviews, and Other Workplace Issues
What Next for the NLRB?
Corporate Law Report: Managing Cyber Risks, BYOD, Obama's NLRB Crisis, Iran Sanctions, and More
The Corporate Law Report: First-to-File Patents, Hiring for Cultural Fit, Roth Conversions Post-Fiscal Cliff, and Global Corporate Insights
Corporate Law Report: Economic Espionage Act, Top FCPA Enforcement Actions, Trademark Audits, and More
How Employers Should Address the Growing Use of Social Media
2007 seems like yesterday.
And yet, eight years after I started this blog and over 1800 posts later (and a Hall of Fame entry), I'm pretty sure 2007 WASN'T yesterday.
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The NLRB's Office of the General Counsel recently published an advice memorandum regarding an employer's social media policy that provides yet another example of the NLRB's disapproval of policies that use overbroad language...more
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In Boch Imports, Inc., the National Labor Relations Board (NLRB) found that the employer, a car dealership, violated the National Labor Relations Act because the dealership's social media and dress code policies were...more
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Over the past several years, EmployNews has reported dozens of cases involving challenges by the National Labor Relations Board's Office of the General Counsel to what appeared to be standard employee handbook provisions. In...more
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One of the biggest misconceptions employers have is that the National Labor Relations Act (NLRA) only applies to unionized employers. As a result, employers may hear of an adverse ruling from the National Labor Relations...more
In 2015, I predict an increased focus on employees' rights regarding their personal social media accounts. Since 2012, individual states have enacted laws prohibiting employers from requesting access to their employees' (or...more
During our workplace privacy segment, our presenters, Mintz Levin attorneys Cynthia Larose and Richard Block, and Vice President, Deputy General Counsel of Time, Inc., Michelle Goldstein, addressed several issues that...more
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Introduction -
If the National Labor Relations Board seemed to be on the ropes in 2013, it certainly came out swinging in 2014. Last year, we reported that the Board faced a number of serious legal battles. Although...more
Social media continues to play an important role in all aspects of a company's internal and public communications. Companies are utilizing social media to make SEC disclosures, coordinate targeted advertising and marketing...more
While the year is still young, here are 15 New Year's resolutions that employers may want to make:
1. Make sure your "independent contractors" are really independent contractors. "Independent contractors" are under...more
In This Issue:
- Message from the Chair
- On the Horizon: Is Obama Remaking the Workplace While Leaving Congress Behind?
- The 2014 Supreme Court Decisions Every Employer Should Know
- What to Expect from the...more
Handbooks in light of several recent legislative actions and judicial decisions. Employers should generally review and update Employee Handbooks as necessary on a yearly basis, but numerous changes will be necessary for most...more
Recent District of Hawaii decision suggests social media policies are more important than ever, despite NLRB's dramatic limits on employers' ability to police employee speech on the Internet -
Howard v. Hertz -...more
In the wake of the NLRB's aggressive crackdown on social media policies, many employers have asked: "Is there any limit to what employees can post on social media about their employers?" It appears that there is. Just last...more
As we reported previously, social media issues are troublesome for employers who must navigate unsettled or even conflicting federal and state laws and decisions. A recent ruling from the National Labor Relations Board...more
The National Labor Relations Board ("NLRB") continues to expand its reach beyond its traditional role involving unionized workforces. In particular, the NLRB has continued an aggressive campaign begun in 2011 to crackdown on...more
Stop me if you heard this one: the National Labor Relations Board recently reinstated employees who were discharged for comments made on their Facebook pages and found that the employer's social media policy was unlawful....more
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Rockstar calls time for GTA Online on Xbox 360 and PS3, halts further updates
Nearly two years after Grand Theft Auto V's online multiplayer was launched, the service appears to be reaching its end-of-life on the older generation of consoles.
State of Decay studio shutting down free-to-play RPG Moonrise
State of Decay developer Undead Labs has announced its RPG Moonrise will shut down at the end of this year.
Games absent from Top 25 US mobile apps chart - report
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Elite: Dangerous Xbox One full release set for October
Elite: Dangerous is heading for full release on Xbox One next month, developer Frontier has announced.
Guitar Hero among a trio of Activision titles announced for Apple TV
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Harmonix announces original Apple TV title Beat Sports
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Ninja Theory says Hellblade hasn't gone back to the drawing board
Having seemingly claimed that it had scrapped much of its work on Hellblade and started over, developer Ninja Theory now insists that the game remains on track.
Forza's tenth lap
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UK eSports specialist Multiplay targets worldwide expansion
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The Amazon Fire Phone is no more
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Nintendo plans Mario Kart 8 and Splatoon-level marketing push for Super Mario Maker
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UK games retail is hopeful that the Q4 excitement will continue into next year thanks to a 'superb' line-up of titles coming to stores in Q1 2016.
AMD spins-off graphics division, will focus on GPUs and VR
AMD's graphics card offering is now a business all of its own.
It's games journalism, but not as we know it
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The gaming credentials of the new Apple TV appear to be somewhat less potent than rumours had led us to believe.
Mobile game Pokémon Go to bring pocket monsters into the real world via GPS
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GameStop could yet hit UK High Street
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Two years after its presumed death, pre-owned is thriving
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Read about the new breed of games journalism in this week's MCV
As consumers turn to an ever-growing number of sources for their games knowledge, the traditional games media has had to change how it reports on the medium.
iOS 9 hits next week
iOS 9 is set to release next week, along with Apple Watch's latest operating system.
Apple unveils new iPhone 6S and iPad models
Apple has revealed the iPhone 6S and 6S Plus, along with two new iPad models.
New Apple TV arrives next month, adds gaming functionality
Apple has officially unveiled its newest iteration of the Apple TV, which will bring gaming into the fold for the digital media player.
Bethesda announces $30 Fallout 4 Season Pass... but no content
A Season Pass has been announced for Fallout 4, even though Bethesda does not yet know what DLC it will make for the game.
The Witcher 3 cost £53,000,000 to make
Developer CD Projekt Red has confirmed the investment needed to create RPG hit The Witcher 3: Wild Hunt.
Minecraft welcomes five-player, cross-platform multiplayer
Microsoft has announced one of the most significant updates ever to be released for Minecraft.


text: cwhp.org [ Central Wyoming Hospice Sets Volunteer Training By Brian Scott August 16, 2014 Central Wyoming Hospice & Transitions is seeking compassionate people to complete ordinary tasks for patients and their families. These t... Read More|Comment Category: Casper News, Morning Tags: central wyoming hospice, fall, training, volunteer Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


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Next Slideshow:
10 foods you should never refrigerate
"There will be live racing. We have 12 races on Saturday and 10 on Sunday," said Karen Weiss of the Maryland Jockey Club.
Horse racing fans hit the infield at Pimlico Race Course in Baltimore for a chance to catch the races and sample some wine at the Decanter Wine Festival.


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Found: The Point of Twitter
WebProNews Staff
Hopefully, we're over wondering what the point of Twitter is and we can move on to how, exactly, using it or other social media can be good for business. Sometimes the biggest obstacle is just wrapping your head around something.
The short and skinny of what is to follow is this:
Understanding humans better benefits business
Think overall strategy, not just tactics
Twitter has good search placement (tactic)
Twitter is a goldmine of permission-based marketing (strategy)
Building relationships also builds trust and opportunities
Niches are inevitable (and present); find yours
One-to-many communication is efficient and on your terms
Previously, I and others suggested Twitter was destined for spamminess, or at least some kind of deceptive marketing. Indeed it is and indeed it has already begun. That's not a reason, though, to avoid it. Use it while it still has value.
Also previously, I waxed existential about Twitter being, for me, a place to observe and learn from humanity. While that doesn't seem immediately useful (if you're old school, it seems like a real waste of time), a very large part of marketing is not just identifying your market, but understanding it better. Twitter provides a perch from which to observe and learn. A new app called Twittearth makes that perch that much more intriguing.
Search Engine Guide's Jennifer Laycock referred to it as "acceptable eavesdropping." From this perch, you can exercise a novel concept in marketing: listening. This is the difference between you and a spammer or spaghetti-against-the-wall message-pounder, always with his mouth open and his ears shut.
"Personally," says Enquiro's Gord Hotchkiss in a blog post on the difference between strategy and tactics, "I've felt that by providing glimpses into user behavior, I can help provide a lens to help see things from the outside in, an essential perspective for strategic evaluation. Part of any strategy in marketing always depends on gaining a deeper understanding of the common denominator, humans."
So what's the difference between a strategy and a tactic? A tactic would include actions like ones used by the aforementioned loud-mouth spammer, who, if we're just talking Twitter, would either never be followed to begin with or would be unfollowed as soon as he betrayed a Twitterer's trust. Or, it could include utility of the knowledge that Twitter, like Wikipedia, now ranks very well in Google's search results.
Rocketboom's Andrew Baron showed us this when he noted that only eight people ranked for "I got a call from eBay." Sure enough, there's more to it than the one freak occurrence. At Shoemoney, AJ Vaynerchuk shows how well Twitter users' personal brands ranked in Google's search results. Twitter profiles appeared on the first page almost without fail. Tactic: Establish a brand on Twitter, a site with high trust (the creator is a Googler, by the way), and you could establish some visibility in the SERPs.
What good does that do you? None if you don't have something to offer. But it could fall into the greater strategy of connecting with people and building relationships. The deeper, harder to crack usefulness of Twitter is via the oft theorized but little focused on concept of permission-based marketing. Twitter followers and followees are in your network by choice, and if you've earned it, they'll listen.
"It also gives you some insight into who companies and bloggers are as people; their likes, their dislikes, their personalities," says Ms. Laycock. "This can be invaluable when it comes to putting together pitches and building relationships both inside and outside of your industry."
In a small survey of online marketers done by SmallBizTrends, two social networks popped up most when asked which were the best for small businesses to use: Twitter and Facebook. That's because they allow you to unobtrusively interact with your market to build relationships and trust within – and here's the important part – your niche. You'll see more and more niche-based networks pop up and those will be useful too, but in the meantime, there's a good chance your niche is already hanging out at Twitter or Facebook or even LinkedIn.
Messages are short and to the point, which people like, and those same people have given you permission to interact with them. It doesn't have to be a marketing message (besides, nobody likes a pitch they can smell), but a genuine relationship to build trust. Think carefully about trust: When buying a car, you'd be more likely to deal with the guy your dad has dealt with for 30 years than you would with the kid that just shook your hand on the lot, right? It works the same way. People are more likely to do business with people they are already familiar with.
And you don't build that familiarity with on-message bombardments people are likely to ignore or resent. You build it by understanding them better and by helping them understand you better. Tweet to tell your audience about recent successes, about what happened on the way to dropping the kids off at school, about your thoughts on current events, about a really kick-butt blog post.
Really, that's the best part about these sprouting one-to-many, permission-based communication channels: It's your message on your terms. Just ask the Prime Minister of Britain, whose team has a Twitter account to deliver his messages without the media's filter/translation/supposed bias. You can wax about whether you can trust anything coming out of a governmental office, but the idea makes a lot of sense. Others have used it for free giveaways and to answer customer questions.
Hopefully, this illustrates how businesses can use Twitter and other social networks in a meaningful, strategic way; hopefully, how you apply the knowledge will foster two-way, permission-based relationships, not marketer-versus-customer tactics. Remember that box you're supposed to think outside of? Welcome to the outside.