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Publications (94)475.22 Total impact
Article: B-Raf Regulation of Integrin 4 1-mediated Resistance to Shear Stress through Changes in Cell Spreading and Cytoskeletal Association in T Cells[Show abstract] [Hide abstract]
ABSTRACT: The regulation of integrin-mediated adhesion is of vital importance to adaptive and innate immunity. Integrins are versatile proteins and mediate T cell migration and trafficking by binding to extracellular matrix or other cells as well as initiating intracellular signaling cascades promoting survival or activation. The MAPK pathway is known to be downstream from integrins and to regulate survival, differentiation, and motility. However, secondary roles for canonical MAPK pathway members are being discovered. We show that chemical inhibition of RAF by sorafenib or shRNA-mediated knockdown of B-Raf reduces T cell resistance to shear stress to α4β1 integrin ligands vascular cell adhesion molecule 1 (VCAM-1) and fibronectin, whereas inhibition of MEK/ERK by U0126 had no effect. Microscopy showed that RAF inhibition leads to significant inhibition of T cell spreading on VCAM-1. The association of α4β1 integrin with the actin cytoskeleton was shown to be dependent on B-Raf activity or expression, whereas α4β1 integrin affinity for soluble VCAM-1 was not. These effects were shown to be specific for α4β1 integrin and not other integrins, such as α5β1 or LFA-1, or a variety of membrane proteins. We demonstrate a novel role for B-Raf in the selective regulation of α4β1 integrin-mediated adhesion.Journal of Biological Chemistry 06/2014; 289(33). DOI:10.1074/jbc.M114.562918 · 4.57 Impact Factor
Article: The PI3-Kinase Delta Inhibitor Idelalisib (GS-1101) Targets Integrin-Mediated Adhesion of Chronic Lymphocytic Leukemia (CLL) Cell to Endothelial and Marrow Stromal Cells[Show abstract] [Hide abstract]
ABSTRACT: CLL cell trafficking between blood and tissue compartments is an integral part of the disease process. Idelalisib, a phosphoinositide 3-kinase delta (PI3Kδ) inhibitor causes rapid lymph node shrinkage, along with an increase in lymphocytosis, prior to inducing objective responses in CLL patients. This characteristic activity presumably is due to CLL cell redistribution from tissues into the blood, but the underlying mechanisms are not fully understood. We therefore analyzed idelalisib effects on CLL cell adhesion to endothelial and bone marrow stromal cells (EC, BMSC). We found that idelalisib inhibited CLL cell adhesion to EC and BMSC under static and shear flow conditions. TNFα-induced VCAM-1 (CD106) expression in supporting layers increased CLL cell adhesion and accentuated the inhibitory effect of idelalisib. Co-culture with EC and BMSC also protected CLL from undergoing apoptosis, and this EC- and BMSC-mediated protection was antagonized by idelalisib. Furthermore, we demonstrate that CLL cell adhesion to EC and VLA-4 (CD49d) resulted in the phosphorylation of Akt, which was sensitive to inhibition by idelalisib. These findings demonstrate that idelalisib interferes with integrin-mediated CLL cell adhesion to EC and BMSC, providing a novel mechanism to explain idelalisib-induced redistribution of CLL cells from tissues into the blood.PLoS ONE 12/2013; 8(12):e83830. DOI:10.1371/journal.pone.0083830 · 3.23 Impact Factor
Article: CD25 and CD69 induction by α4β1 outside-in signaling requires TCR early signaling complex proteins.[Show abstract] [Hide abstract]
ABSTRACT: Distinct signaling pathways producing diverse cellular outcomes can utilize similar subsets of proteins. For example, proteins from the T cell receptor (TCR) early signaling complex (ESC) are also involved in interferon-α receptor signaling. Defining the mechanism for how these proteins function within a given pathway is important in understanding the integration and communication of signaling networks with one another. We investigated the contributions of the TCR ESC proteins Lck, ZAP-70, Vav1, SLP-76, and LAT to integrin outside-in signaling in human T cells. Lck, ZAP-70, SLP-76, Vav1, and LAT were activated by α4β1 outside-in signaling but in a manner different from TCR signaling. TCR stimulation recruits ESC proteins to activate the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK). α4β1 outside-in-mediated ERK activation did not require TCR ESC proteins. However, α4β1 outside-in signaling induced CD25 and costimulated CD69 and this was dependent upon TCR ESC proteins. TCR and α4β1 outside-in signaling are integrated through the common use of TCR ESC proteins; however, these proteins display functionally distinct roles in these pathways. These novel insights into the crosstalk between integrin outside-in and TCR signaling pathways are highly relevant to the development of therapeutic strategies to overcome disease associated with T cell deregulation.Biochemical Journal 06/2013; 454(Pt 1). DOI:10.1042/BJ20130485 · 4.40 Impact Factor
Article: Small Molecule Agonist of Very Late Antigen-4 (VLA-4) Integrin Induces Progenitor Cell Adhesion[Show abstract] [Hide abstract]
ABSTRACT: Activation of the integrin family of cell adhesion receptors on progenitor cells may be a viable approach to enhance the effects of stem cell-based therapies by improving cell retention and engraftment. Here, we describe the synthesis and characterization of the first small molecule agonist identified for the integrin α4β1 (also known as very late antigen-4 or VLA-4). The agonist, THI0019, was generated via 2 structural modifications to a previously identified α4β1 antagonist. THI0019 greatly enhanced the adhesion of cultured cell lines and primary progenitor cells to α4β1 ligands VCAM-1 and CS1 under both static and flow conditions. Furthermore, THI0019 facilitated the rolling and spreading of cells on VCAM-1 and the migration of cells toward SDF-1α. Molecular modeling predicted that the compound binds at the α/β subunit interface overlapping the ligand binding site thus indicating that the compound must be displaced upon ligand binding. In support of this model, an analog of THI0019 modified to contain a photoreactive group was used to demonstrate that when cross-linked to the integrin, the compound behaves as an antagonist instead of an agonist. In addition, THI0019 showed cross-reactivity with the related integrin α4β7 as well as α5β1 and αLβ2. When cross-linked to αLβ2, the photoreactive analog of THI0019 remained an agonist, consistent with it binding at the α/β subunit interface and not at the ligand binding site in the inserted ("I") domain of the αL subunit. Co-administering progenitor cells with a compound such as THI0019 may provide a mechanism for enhancing stem cell therapy.Journal of Biological Chemistry 05/2013; 288(27). DOI:10.1074/jbc.M113.479634 · 4.57 Impact Factor
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ABSTRACT: Disseminated prostate cancer (PCa) cells must survive in circulation for metastasis to occur. Mechanisms by which these cells survive are not well understood. By immunohistochemistry of human tissues, we found that levels of β1 integrins and integrin-induced autophosphorylation of FAK (pFAK-Y397) are increased in PCa cells in primary PCa and lymph node metastases, suggesting that β1 integrin activation occurs in metastatic progression of PCa. A conformation-sensitive antibody, 9EG7, was used to examine β1 integrin activation. We found that β1 integrins are constitutively activated in highly metastatic PC3 and PC3-mm2 cells, with less activation in low metastatic LNCaP and C4-2B4 cells. Increased β1 integrin activation as well as the anoikis resistance in PCa cells correlated with metastatic potential in vivo. Knockdown of β1 integrin abrogated anoikis resistance in PC3-mm2 cells. In agreement with β1 integrin activation, PC3-mm2 cells strongly adhered to type I collagen and fibronectin, a process inhibited by the β1 integrin neutralizing antibody mAb 33B6. mAb 33B6 also inhibited the phosphorylation of β1 integrin downstream effectors, focal adhesion kinase (FAK) and AKT, leading to a 3-fold increase in PC3-mm2 apoptosis. Systemic delivery of mAb 33B6 suppressed spontaneous metastasis of PC3-mm2 from the prostate to distant lymph nodes following intra-prostatic injection and suppressed metastasis of PC3-mm2 to multiple organs following intra-cardiac injection. Thus, constitutively activated β1 integrins play a role in survival of PC3-mm2 cells in circulation and represent a potential target for metastasis prevention.Molecular Cancer Research 01/2013; 11(4). DOI:10.1158/1541-7786.MCR-12-0551 · 4.38 Impact Factor
Article: Synthesis and Evaluation of an Anti-MLC1 × Anti-CD90 Bispecific Antibody for Targeting and Retaining Bone-Marrow-Derived Multipotent Stromal Cells in Infarcted Myocardium[Show abstract] [Hide abstract]
ABSTRACT: A key issue regarding the use of stem cells in cardiovascular regenerative medicine is their retention in target tissues. Here, we have generated and assessed a bispecific antibody heterodimer designed to improve the retention of bone-marrow-derived multipotent stromal cells (BMMSC) in cardiac tissue damaged by myocardial infarction. The heterodimer comprises an anti-human CD90 monoclonal antibody (mAb) (clone 5E10) and an anti-myosin light chain 1 (MLC1) mAb (clone MLM508) covalently cross-linked by a bis-arylhydrazone. We modified the anti-CD90 antibody with a pegylated-4-formylbenzamide moiety to a molar substitution ratio (MSR) of 2.6 and the anti-MLC1 antibody with a 6-hydrazinonicotinamide moiety to a MSR of 0.9. The covalent modifications had no significant deleterious effect on mAb epitope binding. Furthermore, the binding of anti-CD90 antibody to BMMSCs did not prevent their differentiation into adipo-, chondro-, or osteogenic lineages. Modified antibodies were combined under mild conditions (room temperature, pH 6, 1 h) in the presence of a catalyst (aniline) to allow for rapid generation of the covalent bis-arylhydrazone, which was monitored at A(354). We evaluated epitope immunoreactivity for each mAb in the construct. Flow cytometry demonstrated binding of the bispecific construct to BMMSCs that was competed by free anti-CD90 mAb, verifying that modification and cross-linking were not detrimental to the anti-CD90 complementarity-determining region. Similarly, ELISA-based assays demonstrated bispecific antibody binding to plastic-immobilized recombinant MLC1. Excess anti-MLC1 mAb competed for bispecific antibody binding. Finally, the anti-CD90 × anti-MLC1 bispecific antibody construct induced BMMSC adhesion to plastic-immobilized MLC1 that was resistant to shear stress, as measured in parallel-plate flow chamber assays. We used mAbs that bind both human antigens and the respective pig homologues. Thus, the anti-CD90 × anti-MLC1 bispecific antibody may be used in large animal studies of acute myocardial infarction and may provide a starting point for clinical studies.Bioconjugate Chemistry 08/2011; 22(8):1706-14. DOI:10.1021/bc200309h · 4.51 Impact Factor
Article: An anti-PR1/HLA-A2 T-cell receptor-like antibody mediates complement-dependent cytotoxicity against acute myeloid leukemia progenitor cells[Show abstract] [Hide abstract]
ABSTRACT: PR1 (VLQELNVTV) is a human leukocyte antigen-A2 (HLA-A2)-restricted leukemia-associated peptide from proteinase 3 (P3) and neutrophil elastase (NE) that is recognized by PR1-specific cytotoxic T lymphocytes that contribute to cytogenetic remission of acute myeloid leukemia (AML). We report a novel T-cell receptor (TCR)-like immunoglobulin G2a (IgG2a) antibody (8F4) with high specific binding affinity (dissociation constant [K(D)] = 9.9nM) for a combined epitope of the PR1/HLA-A2 complex. Flow cytometry and confocal microscopy of 8F4-labeled cells showed significantly higher PR1/HLA-A2 expression on AML blasts compared with normal leukocytes (P = .046). 8F4 mediated complement-dependent cytolysis of AML blasts and Lin(-)CD34(+)CD38(-) leukemia stem cells (LSCs) but not normal leukocytes (P < .005). Although PR1 expression was similar on LSCs and hematopoietic stem cells, 8F4 inhibited AML progenitor cell growth, but not normal colony-forming units from healthy donors (P < .05). This study shows that 8F4, a novel TCR-like antibody, binds to a conformational epitope of the PR1/HLA-A2 complex on the cell surface and mediates specific lysis of AML, including LSCs. Therefore, this antibody warrants further study as a novel approach to targeting leukemia-initiating cells in patients with AML.Blood 02/2011; 117(16):4262-72. DOI:10.1182/blood-2010-07-299248 · 10.45 Impact Factor
- Cancer Research 01/2011; 70(8 Supplement):1725-1725. DOI:10.1158/1538-7445.AM10-1725 · 9.33 Impact Factor
Article: 2E8 binds to the high affinity I-domain in a metal ion-dependent manner: A second generation monoclonal antibody selectively targeting activated LFA-1[Show abstract] [Hide abstract]
ABSTRACT: The activation of leukocyte function-associated antigen-1 (LFA-1) plays a critical role in regulating immune responses. The metal ion-dependent adhesion site on the I-domain of LFA-1 α(L) subunit is the key recognition site for ligand binding. Upon activation, conformation changes in the I-domain can lead LFA-1 from the low affinity state to the high affinity (HA) state. Using the purified HA I-domain locked by disulfide bonds for immunization, we developed an mAb, 2E8, that specifically binds to cells expressing the HA LFA-1. The surface plasmon resonance analysis has shown that 2E8 only binds to the HA I-domain and that the dissociation constant (K(D)) for HA I-domain is 197 nm. The binding of 2E8 to the HA I-domain is metal ion-dependent, and the affinity decreased as Mn(2+) was replaced sequentially by Mg(2+) and Ca(2+). Surface plasmon resonance analysis demonstrates that 2E8 inhibits the interaction of HA I-domain and ICAM-1. Furthermore, we found that 2E8 can detect activated LFA-1 on both JY and Jurkat cells using flow cytometry and parallel plate adhesion assay. In addition, 2E8 inhibits JY cell adhesion to human umbilical vein endothelial cells and homotypic aggregation. 2E8 treatment reduces the proliferation of both human CD4(+) and CD8(+) T cells upon OKT3 stimulation without the impairment of their cytolytic function. Taken together, these data demonstrate that 2E8 is specific for the high affinity form of LFA-1 and that 2E8 inhibits LFA-1/ICAM-1 interactions. As a novel activation-specific monoclonal antibody, 2E8 is a potentially useful reagent for blocking high affinity LFA-1 and modulating T cell activation in research and therapeutics.Journal of Biological Chemistry 10/2010; 285(43):32860-8. DOI:10.1074/jbc.M110.111591 · 4.57 Impact Factor
Article: Potent in vivo suppression of inflammation by selectively targeting the high affinity conformation of integrin α4β1[Show abstract] [Hide abstract]
ABSTRACT: The development of antagonists to the α4 integrin family of cell adhesion molecules has been an active area of pharmaceutical research to treat inflammatory and autoimmune diseases. Presently being tested in human clinical trials are compounds selective for α4β1 (VLA-4) as well as several dual antagonists that inhibit both α4β1 and α4β7. The value of a dual versus a selective small molecule antagonist as well as the consequences of inhibiting different affinity states of the α4 integrins have been debated in the literature. Here, we characterize TBC3486, a N,N-disubstituted amide, which represents a unique structural class of non-peptidic, small molecule VLA-4 antagonists. Using a variety of adhesion assay formats as well as flow cytometry experiments using mAbs specific for certain activation-dependent integrin epitopes we demonstrate that TBC3486 preferentially targets the high affinity conformation of α4β1 and behaves as a ligand mimetic. The antagonist is capable of blocking integrin-dependent T-cell co-activation in vitro as well as proves to be efficacious in vivo at low doses in two animal models of allergic inflammation. These data suggest that a small molecule α4 integrin antagonist selective for α4β1 over α4β7 and, specifically, selective for the high affinity conformation of α4β1 may prove to be an effective therapy for multiple inflammatory diseases in humans.Biochemical and Biophysical Research Communications 10/2010; 400(4):619-24. DOI:10.1016/j.bbrc.2010.08.114 · 2.30 Impact Factor
Article: Adrenergic modulation of focal adhesion kinase protects human ovarian cancer cells from anoikis[Show abstract] [Hide abstract]
ABSTRACT: Chronic stress is associated with hormonal changes that are known to affect multiple systems, including the immune and endocrine systems, but the effects of stress on cancer growth and progression are not fully understood. Here, we demonstrate that human ovarian cancer cells exposed to either norepinephrine or epinephrine exhibit lower levels of anoikis, the process by which cells enter apoptosis when separated from ECM and neighboring cells. In an orthotopic mouse model of human ovarian cancer, restraint stress and the associated increases in norepinephrine and epinephrine protected the tumor cells from anoikis and promoted their growth by activating focal adhesion kinase (FAK). These effects involved phosphorylation of FAKY397, which was itself associated with actin-dependent Src interaction with membrane-associated FAK. Importantly, in human ovarian cancer patients, behavioral states related to greater adrenergic activity were associated with higher levels of pFAKY397, which was in turn linked to substantially accelerated mortality. These data suggest that FAK modulation by stress hormones, especially norepinephrine and epinephrine, can contribute to tumor progression in patients with ovarian cancer and may point to potential new therapeutic targets for cancer management.The Journal of clinical investigation 04/2010; 120(5):1515-23. DOI:10.1172/JCI40802 · 13.22 Impact Factor
Article: Co-engagement of α4β1 integrin (VLA-4) and CD4 or CD8 is necessary to induce maximal Erk1/2 phosphorylation and cytokine production in human T cells[Show abstract] [Hide abstract]
ABSTRACT: The alpha(4)beta(1) integrin VLA-4 (very-late activation antigen-4) and the lineage-specific CD4 and CD8 receptors have been proposed as putative co-stimulatory receptors on T cells. To assess the relative contribution of signaling through the TCR, CD28 and these accessory molecules, we activated human T cells using soluble antibodies recognizing all four of these T-cell receptor classes (CD3, CD28, CD4/CD8, and VLA-4), and we assessed the degree of activation using higher-order flow cytometry detecting intracellular Erk1/2 phosphorylation and production of IL-2 and IFN-gamma. We found that: (1) co-stimulation via CD4/CD8, in addition to CD28, is required for optimal T-cell activation; (2) VLA-4 binding consistently potentiates CD4(+) and CD8(+) T-cell activation; (3) augmentation of T-cell activation through VLA-4 binding is most pronounced following engagement of CD4/CD8. These results confirm that multiple signals, including VLA-4 engagement, are necessary for maximal T-cell activation beyond that induced via the TCR and CD28.Human immunology 10/2009; 71(1):23-8. DOI:10.1016/j.humimm.2009.09.360 · 2.14 Impact Factor
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ABSTRACT: The Integrin α4β7 mediates lymphocyte adhesion to VCAM-1 on activated endothelium, fibronectin in the extracellular matrix, and the mucosal vascular addressin MAdCAM-1. It is unclear whether α4β7 performs any function beyond directing specific adhesion reactions. We addressed the possibility that triggering of α4β7 with a specific monoclonal antibody was capable of delivering an accessory stimulus that would coactivate T cells and lead to proliferation. At submitogenic levels of anti-CD3 stimulation, triggering of α4β7 by immobilized mAb ACT-1 resulted in T cell blastogenesis, IL-2 production, expression of the IL-2 receptor α chain CD25, and ultimately DNA synthesis. These results indicate that the integrin α4β7 is involved in more than lymphocyte adhesion and homing but also plays a role in cell signaling.Cell Communication & Adhesion 07/2009; 2(6):539-547. DOI:10.3109/15419069409014217 · 1.52 Impact Factor
Article: Cytokines and angiogenic factors in patients with metastatic renal cell carcinoma treated with interferon-α: Association of pretreatment serum levels with survival[Show abstract] [Hide abstract]
ABSTRACT: To correlate serum cytokine and angiogenic factor (CAF) levels with overall survival (OS) in metastatic renal cell carcinoma (mRCC) treated with interferon-alpha (IFN-alpha). Serum CAF levels were measured in 103 patients treated on a randomized trial with IFN-alpha 0.5 million units (MU) twice daily or 5 MU daily. Concentrations of 17 analytes were determined by multiplex bead immunoassays [vascular endothelial growth factor A (VEGF(A)) and several cytokines] or enzyme-linked immunosorbent assay (basic fibroblast growth factor). We used proportional hazards models to evaluate the effect of CAF levels and clinical factors on OS. Pretreatment serum interleukin (IL) 5, IL-12 p40, VEGF(A), and IL-6 levels and Memorial Sloan-Kettering Cancer Center risk grouping independently correlated with OS, with hazard ratios of 2.33, 2.00, 2.07, 1.82, and 0.39, respectively (concordance index = 0.69 for the combined model versus 0.60 for the CAF model versus 0.52 for the clinical model). Based on an index derived from these five risk factors (RFs), patients with 0-2 RF had a median OS time of 32 months versus 9 months for patients with 3-5 RF (P < 0.0001). Serum CAF profiling contributes to prognostic evaluation in mRCC and helps to identify a subset of patients with 20% 5-year OS.Annals of Oncology 06/2009; 20(10):1682-7. DOI:10.1093/annonc/mdp054 · 7.04 Impact Factor
Article: LFA-1 Affinity Regulation Is Necessary for the Activation and Proliferation of Naive T Cells[Show abstract] [Hide abstract]
ABSTRACT: The activation of LFA-1 (lymphocyte function-associated antigen) is a critical event for T cell co-stimulation. The mechanism of LFA-1 activation involves both affinity and avidity regulation, but the role of each in T cell activation remains unclear. We have identified antibodies that recognize and block different affinity states of the mouse LFA-1 I-domain. Monoclonal antibody 2D7 preferentially binds to the low affinity conformation, and this specific binding is abolished when LFA-1 is locked in the high affinity conformation. In contrast, M17/4 can bind both the locked high and low affinity forms of LFA-1. Although both 2D7 and M17/4 are blocking antibodies, 2D7 is significantly less potent than M17/4 in blocking LFA-1-mediated adhesion; thus, blocking high affinity LFA-1 is critical for preventing LFA-1-mediated adhesion. Using these reagents, we investigated whether LFA-1 affinity regulation affects T cell activation. We found that blocking high affinity LFA-1 prevents interleukin-2 production and T cell proliferation, demonstrated by TCR cross-linking and antigen-specific stimulation. Furthermore, there is a differential requirement of high affinity LFA-1 in the activation of CD4(+) and CD8(+) T cells. Although CD4(+) T cell activation depends on both high and low affinity LFA-1, only high affinity LFA-1 provides co-stimulation for CD8(+) T cell activation. Together, our data demonstrated that the I-domain of LFA-1 changes to the high affinity state in primary T cells, and high affinity LFA-1 is critical for facilitating T cell activation. This implicates LFA-1 activation as a novel regulatory mechanism for the modulation of T cell activation and proliferation.Journal of Biological Chemistry 04/2009; 284(19):12645-53. DOI:10.1074/jbc.M807207200 · 4.57 Impact Factor
Article: Clustering T cell GM1 Lipid Rafts Increases Cellular Resistance to Shear on Fibronectin through Changes in Integrin Affinity and Cytoskeletal Dynamics[Show abstract] [Hide abstract]
ABSTRACT: Lipid rafts are small laterally mobile microdomains that are highly enriched in lymphocyte signaling molecules. GM1 gangliosides are a common lipid raft component and have been shown to be important in many T-cell functions. The aggregation of specific GM1 lipid rafts can control many T-cell activation events, including their novel association with T-cell integrins. We found that clustering GM1 lipid rafts can regulate beta1 integrin function. This was apparent through increased resistance to shear flow-dependent detachment of T cells adherent to the alpha4beta1 and alpha5beta1 integrin ligand fibronectin (FN). Adhesion strengthening as a result of clustering GM1 enriched lipid rafts correlated with increased cellular rigidity and morphology through the localization of cortical F-actin, the resistance to shear-induced cell stretching, and an increase in the surface area and symmetry of the contact area between the cell surface and adhesive substrate. Furthermore, clustering GM1 lipid rafts could initiate integrin 'inside-out' signaling mechanisms. This was seen through increased integrin-cytoskeleton associations and enhanced soluble binding of FN and VCAM-1, suggesting the induction of high-affinity integrin conformations. The activation of these adhesion-strengthening characteristics appears to be specific for the aggregation of GM1 lipid rafts as the aggregation of the heterogeneous raft-associated molecule CD59 failed to activate these functions. These findings indicate a novel mechanism to signal to beta1 integrins and to activate adhesion-strengthening processes.Immunology and Cell Biology 02/2009; 87(4):324-36. DOI:10.1038/icb.2008.103 · 4.15 Impact Factor
Article: Distribution of SIBLING proteins in the organic and inorganic phases of rat dentin and bone[Show abstract] [Hide abstract]
ABSTRACT: The SIBLING protein family is a group of non-collagenous proteins (NCPs) that includes dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), and osteopontin (OPN). In the present study, we compared these four proteins in different phases of rat dentin and bone. First, we extracted NCPs in the unmineralized matrices and cellular compartments using guanidium-HCl (G1). Second, we extracted NCPs closely associated with hydroxyapatite using an EDTA solution (E). Last, we extracted the remaining NCPs again with guanidium-HCl (G2). Each fraction of Q-Sepharose ion-exchange chromatography was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Stains-All stain, and with western immunoblotting. In dentin, the NH(2)-terminal fragment of DSPP and its proteoglycan form were primarily present in the G1 extract, whereas the COOH-terminal fragment of DSPP was present exclusively in the E extract. The processed NH(2)-terminal fragment of DMP1 was present in G1 and E extracts, whereas the COOH-terminal fragment of DMP1 existed mainly in the E extract. Bone sialoprotein was present in all three extracts of dentin and bone, whereas OPN was present only in the G1 and E extracts of bone. The difference in the distribution of the SIBLING proteins between organic and inorganic phases supports the belief that these molecular species play different roles in dentinogenesis and osteogenesis.European Journal Of Oral Sciences 05/2008; 116(2):104-12. DOI:10.1111/j.1600-0722.2008.00522.x · 1.49 Impact Factor
Article: CD45RA T-cell activation without proliferation by a partial agonist monoclonal antibody to β1 integrin[Show abstract] [Hide abstract]
ABSTRACT: CD45RA T cells are fully co-activated by natural beta1 integrin ligands fibronectin (FN) and VCAM-1, as well as monoclonal antibody (mAb) 19H8, which binds a combinatorial epitope of the alpha4beta1 heterodimer. These integrin ligands stimulate CD3-dependent proliferation and the upregulation of early activation markers CD25 and CD69. However, beta1-specific antibody 33B6, which binds to a similar range of the predominant T-cell integrins as natural ligands FN (alpha4beta1 and alpha5beta1) and VCAM-1 (alpha4beta1), failed to costimulate proliferation in the CD45RA subset, while retaining the ability to costimulate early activation markers CD25 and CD69. After addition of exogenous human interleukin-2 to the culture media, 33B6 costimulation of proliferation is restored. These data provide evidence that a branch of the alpha4beta1 integrin-signaling pathway in CD45RA T cells can be independently regulated and exploited through the use of partial agonist ligands, including mAbs to the integrin heterodimer.Immunology and Cell Biology 01/2008; 86(4):381-4. DOI:10.1038/sj.icb.7100165 · 4.15 Impact Factor
Article: Diaz-Montero CM, Wygant JN, McIntyre BW.. PI3-K/Akt-mediated anoikis resistance of human osteosarcoma cells requires Src activation. Eur J Cancer 42: 1491-1500[Show abstract] [Hide abstract]
ABSTRACT: Considerable advances in understanding the mechanisms associated with anoikis resistance of normal and malignant epithelial cells have been made. However, little is still known about the pathways involved in anoikis resistance of non-epithelial cells such as fibroblasts and sarcomas. Our results show that Src activity contributes to anoikis resistance of human osteosarcoma SAOS-2 cells. Src was found to be upregulated in anoikis resistant SAOS cells, and pharmacological inhibition of its activity resulted in the restoration of anoikis sensitivity. A normal pattern of dephosphorylation of FAK was observed upon cell detachment of both anoikis sensitive and resistant SAOS-2 cells, suggesting that FAK activity during anoikis resistance is not essential. The activity of Akt was found to be upregulated in anoikis resistant SAOSar cells and the pharmacological inhibition of PI3-K activity restored sensitivity to anoikis resistant cells, reconfirming the critical role of PI3-K/Akt pathway in cell survival. Furthermore, pharmacological inhibition of Src resulted in a decrease of Akt phosphorylation at Ser473. Altogether, these studies indicated a survival pathway mediated by the Src-dependent activation of the PI3-K/Akt pathway in a manner independent of FAK activity.European Journal of Cancer 08/2006; 42(10):1491-500. DOI:10.1016/j.ejca.2006.03.007 · 5.42 Impact Factor
Article: Contrasting Roles for Domain 4 of VCAM-1 in the Regulation of Cell Adhesion and Soluble VCAM-1 Binding to Integrin 4 1[Show abstract] [Hide abstract]
ABSTRACT: Cell adhesion mediated by the interaction between integrin alpha4beta1 and VCAM-1 is important in normal physiologic processes and in inflammatory and autoimmune disease. Numerous studies have mapped the alpha4beta1 binding sites in VCAM-1 that mediate cell adhesion; however, little is known about the regions in VCAM-1 important for regulating soluble binding. In the present study, we demonstrate that 6D VCAM-1 (an alternatively spliced isoform of VCAM-1 lacking Ig-like domain 4) binds alpha4beta1 with a higher relative affinity than does the full-length form of VCAM-1 containing 7 Ig-like extracellular domains (7D VCAM-1). In indirect binding assays, the EC50 of soluble 6D VCAM-1 binding to alpha4beta1 on Jurkat cells (in 1 mM MnCl2) was 2 x 10(-9) M, compared with 7D VCAM-1 at 11 x 10(-9) M. When used in solution to inhibit alpha4beta1 mediated cell adhesion, the IC50 of 6D VCAM-1 was 13 x 10(-9) M, compared with 7D VCAM-1 measured at 150 x 10(-9) M. Removal of Ig-like domains 4, 5, or 6, or simply substituting Asp328 in domain 4 of 7D VCAM-1 with alanine, caused increased binding of soluble 7D VCAM-1 to alpha4beta1. In contrast, cells adhered more avidly to 7D VCAM-1 under shear force, as it induced cell spreading at lower concentrations than did 6D VCAM-1. Finally, soluble 6D VCAM-1 acts as an agonist through alpha4beta1 by augmenting cell migration and inducing cell aggregation. These results indicate that the domain 4 of VCAM-1 plays a contrasting role when VCAM-1 is presented in solution or as a cell surface-expressed adhesive substrate.The Journal of Immunology 05/2006; 176(8):5041-9. DOI:10.4049/jimmunol.176.8.5041 · 4.92 Impact Factor
Top Journals
University of Texas MD Anderson Cancer Center
Houston, Texas, United States
- Department of Immunology
Baylor College of MedicineHouston, Texas, United States
Rice University
Houston, TX, United States
- Department of Bioengineering
University of HoustonHouston, Texas, United States
Stanford Medicine
Stanford, California, United States
- Department of Cardiothoracic Surgery
Johns Hopkins University
Baltimore, MD, United States
- Department of Medicine
Stanford University
Stanford, California, United States
- Department of Pediatrics


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This is the second follow-up piece in my series of why my blog and online presence will enhance future career opportunities. For the introduction click here and for part one about technology click here.
I mentioned this in passing last week, but one of the most incredible things about blogging and social media is the ability to build relationships with people. Not only those you see on a day-to-day basis, but also those you've never met before.
As I write this post, I've recently surpassed 90,000 views on my blog. This may seem small in the scheme of things on the internet, but it was still exciting to me. Every time I post something it is pushed out to my social media network and blog subscribers, that's over 1,800 contacts. And yet these are just statistics. They are not relationships.


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50 Cent back at Daytona looking for a kiss
DAYTONA BEACH, Fla. (AP) – 50 Cent wants to get kissed or die tryin' at Daytona.
The rapper was back for Daytona 500 weekend a year after he tried to plant an awkward smooch on Fox reporter Erin Andrews.
He failed to connect _ but 50 Cent has big plans for his follow up.
"I was looking forward to kissing somebody," 50 Cent said Saturday.
He didn't have to look far. 50 Cent shared a stage with Swan Racing drivers Cole Whitt and Parker Kligerman.
"I'm going to kiss Cole," 50 Cent said.
"Only if I win," Whitt responded.
50 Cent, who launched an ultra-successful, six-times platinum debut with "Get Rich or Die Tryin'" in 2003, was at Daytona to celebrate his latest business deal. He brought his headphones and audio line into NASCAR via a partnership with Swan Racing.
The rapper _ born Curtis Jackson _ will have branding for his SMS Audio line on both of Swan Racing's cars for Sunday's Daytona 500. The logos will also be on Kligerman and Whitt's uniforms, and the drivers will be featured on Swan-branded SMS Audio headphones.
Kligerman took 50 Cent for a wild spin around Daytona in the pace car and hit 135 mph early in the day.
"I didn't know a Camry could do that!" 50 Cent said.
Kligerman, who went airborne and landed on the roof in his first full Daytona 500 practice, kept 50 Cent and the car on four wheels. He was thrilled to be associated with one of his favorite artists. He said his favorite 50 Cent song is "How To Rob."
"That was the one in high school a lot of my friends talked about all the time," Kligerman said. "But for pump-up song, I know you've heard this a lot, but `Many Men.'"
This was just the warm up. Who knows what's in store for race day?
In the strangest part of the buildup to last year's Daytona 500, 50 Cent brought back memories of Joe Namath's awkward attempt to plant one on Suzy Kolber when he tried the same move with Andrews on pit road.
She turned her head one way, then the other, only allowing the "Candy Shop" rapper to get a peck on the cheek.
"I wanted to kiss her, man," 50 Cent said. "She handled it amazingly."
He's such a NASCAR fan, 50 Cent might be willing to spend some big bucks and invest in team ownership.
"I want to belong here. I like it," he told The AP. "I want to have ownership in a team. Someone should look forward to me investing in a team. I just like it. The energy of the actual racing is cool."
He's been busy with his day job.
After battling with his major label for years, 50 Cent has decided to become an independent artist. He left his longtime record label, Universal Music Group's Interscope Records, and Eminem's imprint, Shady/Aftermath.
The Grammy winner and his G-Unit Records have signed a distribution agreement with Caroline, the independent label at Capital Music Group. Capitol is one of the many labels that are part of Universal Music Group.
Jackson is an admitted former crack dealer turned rapper who has built an empire beyond entertainment. He invested early in Vitamin Water and has expanded his business interests into clothing and now audio equipment. Going independent was just another business decision.
"I can make deals now that I couldn't make under that structure," he told The AP. "On the brand extension, it's obvious I've been the leader in that actual area. When I fell in love with hip-hop culture, it was actually the opposite, it would be selling out. To be an artist and be associated with a major corporation was a crossover. I had enough of a bad-boy image at that point to do what I wanted to do and knew it was bigger business-wise."
50 Cent is about ready to drop his next album, "Animal Ambition." The first songs he plans to release are called "Don't Worry About It," and "Smoke."
Smoke, like Tony Stewart?
"Nah, this one was produced by Dr. Dre."
Dr. Dre produced songs on 50 Cent's new album, perhaps triggering the latest hip-hop rivalry: Which rapper has the freshest headphones, Beats by Dr. Dre or SMS Audio?
"I'm listening to mine, he's listening to his," 50 Cent said, laughing.
Before he left the DIS media center, 50 Cent met actor Gary Sinise of "Forrest Gump" fame and yelled, "Run Forrest!" before they shook hands.
(Copyright 2014 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


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Beyonce releases new song 'Bow Down / I Been On': Listen
"I took some time to live my life, but don't think I'm just his little wife," sings Mrs. Shawn Carter, i.e. Beyonce, on "Bow Down/I Been On," a track she dropped via SoundCloud on Sunday.
[More after the jump...]
The trap- influenced track opens with a heavily-manipulated voice as a not-so-sugary sweet kid before Beyonce comes on full of attitude and ego, telling us "This is my shit/Bow down, bitches." There's only about 40 seconds of Beyonce sounding like Beyonce in the entire 3:34 minute track before the song segues into "I Been On," which features Beyonce's vocals, chopped and screwed and manipulated to sound like a man's, rapping about being all that. She's clearly in a mood to salute her Houston hometown.
Hit-Boy, who also produced Jay-Z and Kanye West's "Ni**as in Paris" and Lil Wayne's "Drop the World," produced "Bow Down/I Been On." He's now working with Britney Spears on her next album.
Fans have already taken the track and unpitched her voice so you can hear here (h/t MTV) what she really sounds like on the "I Been On" rap as she says "smack that trick." "Tell me who's going to take me off because I been on," she says before giving out a shout out to Willie D and to the late Pimp C and the UGK (Underground Kingz)
The song, the first new music we've heard from Bey since 2011's "Four," is all posturing and it's hard to think it's anything more than a buzz song to get us talking about Beyonce because it's been all of three minutes since we were, right? If It's a first single, it's not a strong one at all.
Beyonce's Mrs. Carter World Tour starts June 28. No word on the release date for the next album, although we expect it will be before then.
2008 | Science Fiction | PGSummary: Animated series continues the story of Obi-Wan Kenobi and Anakin Skywalker as they battle the Emperor Palpatine, Count Dooku and General Grievous, but also takes time to explore other smaller characters in the Star Wars universe.Director: George Lucas (creator)
Cast: Tom Kane, Dee Bradley Baker, Matt Lanter
2013 | Thriller | RSummary: Based on the true story of Daniel Lugo (Mark Wahlberg) a Miami bodybuilder who wants to live the American dream. He would like to have the money that other people have. So he enlists the help of fellow bodybuilder Adrian Doorbal (Anthony Mackie) and ex-convict, Christian bodybuilder Paul Doyle (D...Director: Michael Bay
Cast: Mark Wahlberg, Dwayne Johnson, Anthony Mackie, Tony Shalhoub
2007 | Comedy | PGSummary: Newlyweds Nick (Ice Cube) and Suzanne (Long) decide to move to the suburbs to provide a better life for their two kids. But their idea of a dream home is disturbed by a contractor (McGinley) with a bizarre approach to business.Director: Steve Carr
Cast: John C. McGinley, Ice Cube, Nia Long, Aleisha Allen
2013 | Drama | RSummary: Leonardo DiCaprio and Jonah Hill have boundless energy in the story of a real-life commodities crook who earned millions through scummy small-time stock trades.Director: Martin Scorsese
Cast: Leonardo DiCaprio, Jonah Hill, Margot Robbie
2013 | Comedy | NRSummary: Insanely funny comedy show created by Amy Schumer, who stars in brilliantly funny sketches about sex, city living, dating, and friendship.Director: Daniel Powell, Amy Schumer (creators)
Cast: Amy Schumer, Kevin Kane, Mike Houston
1996 | Crime | RSummary: Jerry, a small-town Minnesota car salesman is bursting at the seams with debt... but he's got a plan. He's going to hire two thugs to kidnap his wife in a scheme to collect a hefty ransom from his wealthy father-in-law. It's going to be a snap and nobody's going to get hurt... until people start ...Director: Joel Coen
Cast: William H. Macy, Frances McDormand, Steve Buscemi, Peter Stormare
1997 | Crime | RSummary: Quentin Tarantino adaptats an Elmore Leonard novel into this story of a few increasingly desperate people scraping to get by.Director: Quentin Tarantino
Cast: Pam Grier, Samuel L. Jackson, Robert Forster
1995 | Mystery | NRSummary: Denzel Washington plays an out of work WWII vet who takes the wrong job and is soon neck-deep in a mess of politics, murder, and jazz in '40s Los Angeles.Director: Carl Franklin
Cast: Denzel Washington, Tom Sizemore, Jennifer Beals
1993 | Sports | PGSummary: Emotionally powerful sports classic featuring Sean Astin as a skinny high school kid with big football dreams and the determination to make his way towards his dream team at Notre Dame.Director: David Anspaugh
Cast: Sean Astin, Jon Favreau, Ned Beatty
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The first nighttime care specially dedicated to the eye contour for the moisture lost at night. A combination of exceptional and specific active ingredients for the night in order to repair and reduce lines. When you are lying down, toxins and water tend to stagnate and cause the face and eye contours to look puffy. Escin and caffeine heighten the waste and toxin elimination process that mainly takes place at night to drain, detoxify and decongest. It purifies and eliminates toxins that cause a "puffy" and congested face in the morning. It intensifies nighttime regeneration of the eye contour, repairs wrinkles and purifies. In the morning, wrinkles are softened and the eyes appear younger looking, the complexion looks fresh, the skin purer and contours more defined.


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From small villages to influences, our chat with two Bastillians turned out lively with surprise visitors and more. Watch the video where they reveal one of their idols is Rodney Rotten. Then hear RR's interview from the previous day.
May 262014
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If you've been to any concert in Los Angeles over the years, you surely have spotted our colleague Andrew Youssef, AKA Amateur Chemist, capturing the concert moments with his camera. Shooting for his blog Amateur Chemist, as well as the O.C. Weekly, Stereogum, and other numerous publications, he has been a staple in the photo pit—along with being a great guy to work with. Unfortunately, Andrew Youssef was diagnosed with Stage IV colon cancer three years ago, which has limited his ability to cover the music scene.
Continue reading "Help NIN Fan / Photographer Andrew Youssef" »


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Poster for the National Pike Arts and Music Festival that is taking place this weekend in good ole' Western Maryland. You can see the whole poster in the attachment.
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The Style Gallery is a great new way for you to view, love, and share outfit photos. It's meant to showcase
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See it to Bow-lieve It Flat in Pink
Every time you catch a glimpse of these pastel pink flats, you just cant believe how adorable they are! With a darling display of decorative bows and gold studs atop their T-straps, these vegan faux-leather cuties are nearly too good to be true!
Catch You On the Skip Side Flat in Warm
You just cant help it - every step you take in these watercolor-inspired flats is a buoyant bounce! Accentuated by a darling bow and petal-like brushstrokes in magenta, mint, and ivory hues, these vegan faux-leather slip-ons have you walking on air.
Creature Presentation Flat in Unicorn
And now for the worlds cutest flats on which to feast your eyes! Starring a sweet-as-can-be embroidered unicorn face supported by black piping and a rainbow horn, these fuchsia, vegan faux-leather kicks are a pair your street style followers would gladly pay admission to see.
Creature Presentation Flat in Sheep
And now for the worlds cutest flats on which to feast your eyes! Starring a sweet-as-can-be embroidered sheep face supported by sweet pink piping, these ivory, vegan faux-leather kicks are a pair your street style followers would gladly pay admission to see.
Show 'Em Who's Gloss Flat in Petal
Every time you flaunt these glossy flats, you assert your claim to stylish fame! With cognac brown, vegan faux-leather straps and a comfortably cushioned footbed, these petal pink peep toes boast fashion-forward panache.
Dapper Days Flat in Chocolate
From modish mornings to dashing evenings, youre a classic act in these rich brown Oxford flats. Crafted with pebbled vegan faux leather, pinked and perforated trim, and a low heel, this retro pair suits your stylish ways perfectly.
Dapper Days Flat in Cappuccino
From modish mornings to dashing evenings, youre a classic act in these taupe-brown Oxford flats. Crafted with pebbled vegan faux leather, pinked and perforated trim, and a low heel, this retro pair suits your stylish ways perfectly.
Tweed I Say More? Flat
As you can see from the chic silhouette and flirty fabric of these dOrsay flats, their panache speaks for itself! Petal pink, black, and white boucle stitching adorns this pointed-toe pair boasting volumes of statement-making style.
Like Two Peas in the Quad Flat
What a lovely couple the two of you make, strutting through campus - we mean you and this mocha brown pair of flats, of course! Smartly detailed with perforations, glossy pinked piping, and earthy plaid trim, these Oxfords are your pair-amour to adore from the first week to finals.
Rosette You Straight Flat
Should you feel at a loss for what to wear one day, these dOrsay flats will guide you in a glam direction! Pink roses perfectly blossom atop the black fabric of this pointed-toe pair - making these shoes a fashion choice thatll allow the rest of your ensemble to fall ...
Fanfare Arrival Flat
You touch down in England with bells on, as evident by your choice to sport these Anglophile flats! Patterned with Great Britain-themed motifs in khaki, red, blue, and tickled pink - and fancied up with bows at the toes - these canvas-like flats will have you traveling feeling chuffed to ...
By Word of South Flat in Cool
From Albuquerque to El Paso, word of this zesty printed pair is spreading at the speed of 'delight!' Gorgeously geometric, these bow-topped ballet flats boast navy, ivory, ash grey, and petal pink hues that pack a punch of southwestern panache with every endeavor.
The Bright to Remain Stylish Flat
Turn to the brilliant hues and patterns atop these fun flats to perpetuate your bold style choices! With a pleated surplice detail at the toe cap and glowing with turquoise, white, lime, black, cocoa, and neon pink stripes, this pair will have everyone heralding your unique taste.
Fleur-footed Flat
Root your ensemble in these embroidered fabric flats for fail safe style that all are sure to adore! Touched at the toes with glossy grey hearts and rhinestone-kissed bows, this pink, blue, yellow, and white pair displays a fantastically floral silhouette. No fashion slip-ups could possibly occur with these shoes ...
Garden Path Panache Flat
Strolling through the sunflowers always puts a pretty pep in your step, but its these floral flats that really elevate your elation! Hues of cornflower blue, rosy pink, and spring green pattern this pointy-toed pair, while black vegan faux-leather straps make these shoes even more distinct.
Tip Tap Toe Flat
Whether youre making a quiet entry into a meeting or tappin along to your favorite tune on the radio, these fab flats have got you covered on panache. Boasting a magenta, navy, and turquoise plaid pattern, these dOrsay slip-ons are prepped to make a statement about your style, no matter ...
Conservatory Concerto Flat In Navy
Ready to impress at your piano recital, you give a chic sashay across the stage in these crocheted lace flats! Piped with navy vegan faux-leather, adorned with an ivory and magenta floral underlay, and complete with glimmering gold accents, this precious pair earns itself an encore.
Tread Ahead Flat
Your fashion sense and line of thought share one significant quality - theyre both forward-thinking, exemplified in the details of these treaded Minnetonka flats! A collection of brightly hued, embroidered diamonds detail the toes of these fringed mocs, giving a fresh update to a charming classic.
Love and Appreciation Flat in Pink
On your busiest days, youre especially grateful for these darling muted rose flats. Thats because these vegan faux-leather lovelies have so much to admire including their classic Mary Jane-esque design, adjustable silver-buckled ankle straps, and tear-shaped cutouts at the rounded toes and counters. These ready-to-style shoes - matched with a ...
Off-Screen Darling Flat in Petal
The audience loves you on-screen, but your day-off looks are what youre known for! These delicate lace flats from Restricted boast a petal-pink hue and grosgrain ribbon and look stellar beneath dark skinnies and a striped top. Don them and add airy delight to errands or a lunch date and ...
Unmatched Charm Flat in Stone
Only you could take a shoe as simple as these grey flats and make them amp up every ensemble! The pinked trim, brogue accents, and buckle-adorned toes of these vegan faux-leather shoes only ever look entirely sophisticated on you.
On Easy Sweet Flat in Fuchsia
Youll be en route to adorable style the moment you slip into these bright flats! Trimmed with matching topstitching and charming cutouts, these supple vegan faux-leather slip-ons are a cute and comfy choice to wear wherever it is youre headed.
Vivid It Up Flat in Roses
Live your life with spirited vibrance in these black flats! A buckled ankle strap charms atop this pairs patent vegan faux-leather heel, while blush, emerald, and magenta flowers dazzle on a pointed toe to embolden your look with captivating color!
Washington Square Spark Flat in Coral
When its sunny in Philadelphia, buckle into this charming cutout pair and set out to catch some rays! These coral, twirl-detailed flats tout a vegan faux-leather construction and thoughtful detailing that pair perfectly with rekindling connections at the park.
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text: Students in the SOMI have several choices regarding curriculum tracks. All sophomore (year 2) students follow the same curriculum. At the end of the sophomore year, students may elect to continue in the radiography track or apply to the sonography track. Sonography will be limited enrollment and selection criteria is primarily based on MI coursework GPA. In the event of a tie, MU science courses and MU GPA will be used as the determining factor. Senior students (year 4) in either sonography or radiography have additional options. Radiography students will select from one of five advanced modality tracks. Sonography will select ABD/OB-GYN or Vascular/Echo. General Education requirements from Marshall University may change. Students should consult with the College of Health Professions at MU to determine any general education requirements. Students are required to complete both MU and SMMC graduation requirements prior to receiving their certificate from SMMC. After January 2015, applicants to the ARRT certification exam must also have the minimum of an AAS degree. The BS degree also meets an ARDMS requirement. Registered technologists wishing to complete the BSMI degree may enter the program in the fourth year.


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Presently, the SZSE Investor Text Message Service Platform was officially put into use, and the SZSE comprehensive connection platform for investors has been preliminarily built up, covering many a channel such as telephones, mails, letters, text messages, online interaction, investor opening day, investor investigation and so forth.
(Paris, 28 September 2009) The World Federation of Exchanges (WFE) Secretariat is pleased to announce the winner of the first annual WFE Award for Excellence, Prof. Robert A. Schwartz of the Zicklin School of Business at Baruch College, CUNY. The WFE Award for Excellence is presented annually in recognition of an outstanding contribution to the advancement of regulated exchange markets. The Award salutes innovation and improvement in the exchange industry's operations which leads to greater understanding of capital markets.
Oslo Børs has chosen Scila Surveillance for their derivatives market. This makes Oslo the fourth market to take in the recently launched all-purpose surveillance system.
Buenos Aires Stock Exchange President Adelmo Gabbi has been appointed President of the Ibero-American Federation of Exchanges (FIAB), at the XXXVI FIAB General Assembly recently held in Lima, Peru.
Bolsas y Mercados Españoles (BME) has created the IBEX 35® IMPACTO DIV, which is meant to act as an underlying asset for derivative products. The index will start to be calculated and disseminated on September 30th.
Athens Exchange and Istanbul Stock Exchange jointly announce the issue of a common index. The Greece & Turkey 30 Index (GT-30), which is a customized index will be calculated, maintained and distributed by STOXX Limited, the leading provider of European equity indexes.
Toronto Stock Exchange ("TSX"), a member of TMX Group (TSX: X), announced that it has received approval from the Ontario Securities Commission ("OSC") for changes to its rules governing public company acquisitions. Effective November 24, 2009 (60 days from today), TSX listed issuers will be required to obtain security holder approval for public company acquisitions that will result in the issuance of 25% or more of their issued and outstanding securities (on a non-diluted basis).
The International Securities Exchange (ISE) announced that dual currency conventions are now available for four currency pairs in its portfolio of ISE FX Options. The "in US$" currency convention, typically used in the FX spot market, is now available alongside ISE's USD-based currency convention for options on the Australian dollar, British pound, Euro and New Zealand dollar.
NYSE Euronext (NYX) announced that its proposal for the expansion of the Securities and Exchange Commission's (SEC ) Penny Pilot program, filed by NYSE Arca options, has been approved for market-wide implementation by the SEC . The approval will extend the proposed pilot through December 31, 2010, including four quarterly additions to the pilot consisting of 75 issues each.
Jasdaq Securities Exchange, Inc. (Jasdaq) started using a trading system of Osaka Securities Exchange Co., Ltd. (OSE).


text: Any tips on cooking a leg of lamb for Easter dinner; a traditional dish in Rome, Italy! Wish me luck!! Share! Tweet Posted by: fleecarlin , April 5, 2012 fleecarlin About the author Related Posts DIY Tomato Beauty Tips Use summer's bounty in your beauty routine with these at-home recipes. The Case for Summer Shorts You can still wear shorts, we promise! Just follow these tips and trips for the perfect look. Moms Give Advice: Long Distance Travel Survival Tips What in the world do you do with the kids while traveling? Don't Derail: Detox After a Holiday Weekend Simple detoxifying steps to beat the bloat after a long weekend. Leave a Reply Cancel reply You must be logged in to post a comment.


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North Heights Recreation CenterRead MoreNature lovers will appreciate the convenience and beauty of North Heights Recreation Center in North Little Rock, a local park. If you're looking for an effective strength-building and cardio workout, MMA is for you. Parking is plentiful, so guests can feel free to bring their vehicles.4801 Allen StNorth Little Rock, AR US


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Moving to or from Jarrell, TX, 76537? Get FREE truck rental rate quotes at Sonterra Self Storage. U-Haul rental trucks are specifically engineered from the ground up to assist moving families, not freight. Our moving trucks have more safety features than other moving trucks in the industry including gentle ride suspension and high visibility mirrors to assist the driver. Whether you are moving a smaller apartment or a large four-bedroom house, U-Haul truck rentals in Jarrell, TX will provide you with the moving truck rental you need to get from point A to point B. Find the perfect size moving truck to assist with your move, U-Haul is home of the $19.95 rental truck!
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I was a little disappointed that I was unable to pick up the truck at my requested location, but customer service worked with me to find a suitable substitute.
The location is convenient but the hours where someone is onsite is difficult to determine. I called the number to see when I could drop off the trailer but there was no one present when I dropped it off. The email response afterward was very prompt.
Y'all were a pleasure to deal with and took a lot of stress out of my day, thank you again. John Miller
When we walked through the door, there was one man sitting at the desk. He did not acknowledge or even look at us and this was at 10am. I said hello and that we had a reservation at 10 for a 26 ft UHaul which I have a confirmation email stating so. The first thing he said was "we don't have a 26ft we only have a 20" I said "What do you mean? The woman called me and said this was the only location with a 26ft so she reserved it and I have an email." He only repeated himself and asked for my last name under the reservation. Then I told him we'll take the 20ft. We really had no choice because over the phone I was told there wasn't a 26ft available within 70 miles of my location. As he was entering my information, he kept tapping keys are, sighing in anger, then the phone rang and he was really rude over the phone (I'd be shocked if that customer didn't file a complaint) and he didn't say "Bye have a good day" or try to be helpful and just hung up and slammed the phone on the desk where it slid to the other side. I was truly appalled that this agent was acting how he was to myself and then to the customer over the phone in front of a customer (Myself). So rather than giving him an attitude back (As he looked like he would lost it or explode) I asked "Having a bad day?" He then relaxed a little, I think he realized his actions, and said "Yes, very bad start. I came in to the roof leaking, the internet keeps going down and out of all days that we have no one, all of a sudden everyone is coming in to rent a truck, and I'm hungover from last night"......Then he continued on how he hates the uHaul company that they always set their location up for failure. He couldn't complete our contract info on the computer due to the intermittent connection and called our drop off location to enter our details. He gave me a key and contract for the truck without verifying my ID or having me sign anything. I'm a corporate employee and I know the expectations of customer regardless the agents personal emotions. Customer satisfaction should be number 1 when you clock in. Everything else wrong, the customer shouldn't even have a clue that there is something wrong with you. This location was very very poor.
The equipment was rented in Cedar Park. A couple of days before were needed the truck and trailer, we were told to pick it up in the Anderson Mill location on Hwy. 183. At 8 pm the night before we were to pick it up, we were told by phone that we had to go to Jarrell, and that they closed at 2 pm. We lived in Lago Vista--a very long drive. Wife wasn't available to drive me to Jarrell, so I had to pay someone to take me. Very inconvenient, and I was not happy about it. Someone should have brought the truck and trailer to the Cedar Park location--very bad customer service.
Jeff Was Great.
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text: Today, I joined President Obama at M. Luis Construction in Rockville as he talked about the impact that the Federal government shutdown is having on our businesses, our communities and countless individuals all across our nation. As the President said, a small group of extremists have manufactured a crisis to create a "reckless and damaging shutdown." This government shutdown is bad for our economy, bad for our County and bad for our nation. In Montgomery County alone, we know the shutdown is affecting about 70,000 federal government employees and is costing the County at least $760,000 a day – not including revenues related to other fees and taxes, impacts on contractors and the businesses that serve the Federal government and its employees. Our local economic recovery is threatened when so many of our residents are without a paycheck. These hardworking employees deserve better – and so do Americans all over the country who benefit directly from their work. I call on the obstructionists in the House of Representatives to stop holding the American people hostage and stop the shutdown.


text: Kanye West Las Vegas Rant by Hilton Hater at October 28, 2013 11:38 am. Comments Kanye West is back to ranting! Watch him go off to a Las Vegas crowd here about the drug of Yeezus. Share Tweet Email Edit Delete Follow Kanye West? Facebook Disqus Star: Kanye West Related Videos: Kanye West Videos Related Post: Kanye West Rants in Las Vegas: F-ck Racism! The Truth is Inside You! Uploaded by: Hilton Hater Uploaded: October 28, 2013 Duration: 281 seconds


text:
Tivoli Audio i-Pal
Well built, decent tuner, good speaker
The price
So you bought your new iPod and want to share the music with all your friends. There are plenty of options from the inMotion speaker set to Creative's portable speaker options, Tivoli Audio are hoping to pip both to the post with the introduction of its restyled player to fit in with the iPod - the iPal. Described on the back of the unit as a Portable Audio Laboratory the results aren't far off.
A solid unit the size of a stubby house brick and weighing like one (it actually weighs 2lb - the same as a bag of sugar), the unit has a well made feel to it - first point scored. The second is that rather than just offering you the option of outputting your music from your iPod, Tivoli Audio has built in an FM/AM tuner into the mix.
Featuring a large dial that takes centre stage on the front of the player, the radio is not only easy to tune, it offers a 5:1 ratio tuning dial, but also produces a good reception without the need of the large aerial on the rear of the unit.
Those not interesting in the radio side of things can opt to simply plug their iPod or other portable MP3 or CD player into the "aux in" socket at the rear. Sound is pumped out from a single speaker at the front and the quality is very good. Sound is well rounded, not too tinny or bassy and more importantly capable of getting quite a clear loud sound. We found best results were achieved by letting the speaker do all the work and tuning down the volume to a low setting on the iPod or MP3 player we tested it with.
The whole device is powered by a NiMH battery, which Tivoli Audio says will charge in 3 hours. You can opt to have the charger plugged in all the time, but that sort of defeats the object. What doesn't defeat the object however is the rubberised seals on the rear of the player over the "aux in", headphones and power in sockets. Why are they rubberised? So you can take it outside in the fine English weather that's why. While not weather proof, it is weather resistant meaning it will withstand the odd spot of rain but not a full blown gale and rain storm.
This player has it all when it comes to providing either radio or speaker output for your iPod or MP3 player. Whether it's the Henry Kloss (he co-founded Cambridge soundworks) tuning dial, or the included white cable to connect your player, the unit has been well built and well executed. The box suggests using an iTrip in the mix to save the wire and with the precision of the tuning dial it makes sense. This is one gadget we are hoping we get to keep. What's the catch? The price, at £130 this is more than the inMotion set and twice the price of Creative's offering.
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text:
Gift Certificates can be used in the restaurant or in the hotel. Select multiple quantities to create a custom amount.
An elegant customized gift certificate will be mailed to the recipient.
27 Mine Brook RoadBernardsville, NJ 07924Directions
Complimentary upgradesfor 2016 weddings
Copyright © 2014 The Bernards Inn. A Hampshire Destination Property


text:
Healthcare Providers and Services
Company Overview of Catamaran Corporation
Executive Profile
Mark A. Thierer
Chairman and Chief Executive Officer, Catamaran Corporation
|Total Calculated Compensation
|This person is connected to 5 Board Members in 5 different organizations across 9 different industries.
See Board Relationships
As of Fiscal Year 2014
Mr. Mark A. Thierer serves as the Chief Executive Officer at Catamaran PBM of Illinois II, Inc. Mr. Thierer serves as President and Chief Executive Officer at Catamaran PBM of Illinois, Inc. He has been the Chief Executive Officer of Catamaran Corporation (Alternate Name: SXC Health Solutions, Corp.) since June 30, 2008. Mr. Thierer served as the Chief Executive Officer and President of Catalyst Health Solutions, Inc., since July 2012. He served as Chairman, President ...
Corporate Headquarters
1600 McConnor Parkway SchaumburgPhone: 800-282-3232
Schaumburg, Illinois 60173
United States
Fax: 224-231-1901
Board Members Memberships
Director and Member of the Advisory Board
Chief Executive Officer and Chairman
Chairman and Chief Executive Officer
Director and Member of Compensation & Leadership Development Committee
Nova Southeastern University
Nova Southeastern University
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text:
Adam Vinatieri's 5 FGs help Colts top Titans, extend AFC South lead
INDIANAPOLIS -- Adam Vinatieri still has it. Donald Brown does, too.
When both delivered throwback performances Sunday, it lifted the Indianapolis Colts closer to the playoffs.
Vinatieri, the oldest player in the league tied a career-high with five field goals and matched an NFL record with four 40-plus yard field goals in one game.
Brown, the Colts' forgotten 2009 first-round pick, scored on a 4-yard TD run with 1:56 left to give Indianapolis a 22-14 victory over Tennessee.
"Those are by no means chip shots," Andrew Luck said after another troubling offensive showing with only 264 total yards. "To have a guy like that (Vinatieri), it, well, it really makes a difference."
Indy (8-4) has a three-game lead over the Titans (5-7) with four left and a sweep of the season series. The Colts can wrap up their eighth AFC South title in 11 seasons with one more win, one more Titans loss or a tie by either team.
It hasn't been easy reaching this point, especially after losing five offensive starters with season-ending injuries, and with Trent Richardson struggling.
So coach Chuck Pagano shook up things after two blowout losses in the last three weeks. He surprised many by moving Brown into the starting lineup ahead of Richardson, the No. 3 overall pick in 2012 by Cleveland who Indy traded for in September. Pagano replaced Mike McGlynn at right guard with Jeff Linkenbach and also started cornerback Darius Butler ahead of Cassius Vaughn.
Vaughn picked off two of Ryan Fitzpatrick's passes, and Brown continued to beat up on the Titans despite rushing for only 8 yards in the first 52 minutes. He had 46 yards on the final drive and gave Indy its only touchdown.
"I guess it was time to put something together and with the division hanging in the balance, the guys manned up," Pagano said, explaining that Brown earned a rare start and the two backs would both continue rotate. "Just like the first game down there, we were able to run the ball, chew up the clock."
They needed Vinatieri even more than Brown.
The soon-to-be 41-year-old resembled his much younger self, tying a career high with four field goals in the first half and matching his full-game career high when he hit a perfect 49-yarder for a 15-14 lead late in the third quarter. His fifth field goal also equaled Jason Elam's NFL record with a 16th 100-point season, and he joined Morten Andersen as the only players to score 800 points with two teams. He's the 10th player in league history to make four 40-yard field goals in a game.
To Vinatieri, it was just another work day.
"You never know how it's going to go, and you don't know how you're going to be needed and used," he said. "Today was one of those days that I was needed and used a bunch."
Without him, the Colts might be lamenting their first back-to-back losses since December 2011.
Luck was just 17 of 32 for 200 yards with no TDs and one interception.
Fitzpatrick wasn't much better. Though he scored on a 1-yard plunge in the first half and gave the Titans a 14-12 lead in the third quarter on an ugly 1-yard TD pass to Chris Johnson, Fitzpatrick was 21 of 37 for 201 yards with one TD. Johnson managed only 69 yards on 18 carries.
"I didn't play well. We had a shot at the end there," Fitzpatrick said. "I didn't get the job done, and it hurts. For me to come out and not play well hurts."
The miscues proved costly.
Tennessee gave away three points just before halftime when Moise Fokou's personal foul extended the half by one play. Vinatieri cashed in with a 37-yarder.
Then, after taking the 14-12 lead, Fitzpatrick had the Titans in scoring position again until NFL sacks leader Robert Mathis stripped him of the ball. Jerrell Freeman scooped it up and ran it to the Titans 32. Four plays later, Vinatieri hit the 49-yarder to give Indy the lead for good.
"It puts us in a spot now where we've got to win four games in a row," coach Mike Munchak said. "We have no control of what that may or may not mean."
Fitzpatrick was picked off two more times before Brown sealed it with his strong run up the middle.
"Pros grind it out," Brown said. "They take it one week at a time and just keep rolling along."
Game notes
The Titans lost two more tight ends Sunday. Delanie Walker left with a concussion in the first quarter and Collin Mooney was taken by golf cart into the locker room with what appeared to be a left leg injury in the second half. ... Indy defensive tackle Ricky Jean Francois (sprained foot) and running back Daniel "Boom" Herron (strained pectoral) should have MRIs on Sunday night, Pagano said. ... Kicker Rob Bironas joined Al Del Greco as the only Titans in the NFL's 1,000-point club.
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Antonio Cromartie waiting for more from Odell Beckham Jr.
Jets cornerback Antonio Cromartie added fuel to the New York rivalry when he explained why he thinks Giants star wideout Odell Beckham Jr. is a "one-year wonder."
Bill O'Brien names Texans QB Brian Hoyer starter for Week 6 vs. Jaguars
Brian Hoyer is the Houston Texans' starting quarterback for the team's Week 6 game against the Jaguars. For how long he remains beyond that game is uncertain.
Marshawn Lynch out for consecutive games for first time with Seahawks
The Seattle Seahawks have ruled running back Marshawn Lynch out for Sunday's game against the Cincinnati Bengals due to a hamstring injury.
Andrus Peat prepares for first career start with Terron Armstead injured
New Orleans Saints first-round draft pick Andrus Peat will make his starting debut at left tackle against the Eagles in place of injured Terron Armstead.
Survey says: Stephen Gostkowski is the best kicker in the league
Stephen Gostkowski of the New England Patriots is the best kicker in the NFL, according to an NFL Nation survey of his peers.
Martha Ford is 'absolutely' in charge of Lions, Bill Ford Jr. says
Detroit Lions vice chairman Bill Ford Jr. said Friday on WJR Radio that his mother, owner Martha Ford, is in full control of the franchise.
2015 AFC South Standings


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Town Square
TV30 offering viewers chance to win gift cards, theater tickets in October
Original post made on Sep 23, 2013
At the Firehouse Theater in Pleasanton, Tri-Valley TV will be giving away tickets to Gypsy Soul on Friday, Oct. 4; School of Rock Live! Jr. on Friday, Oct. 18, and Creatures of Impulse NIGHTMARE on Friday, Oct. 25. TV30 viewers can go to the Tri-Valley TV website at www.trivalleytv.org to register to be a "Lucky Fan." There is no charge and the information will not be shared. TV30 then randomly selects a winner at various times from the "Lucky Fan" registrations. The winner will be announced on the TCTV Facebook page and on TV30. Viewers can enter as many times as they want until they win. For complete rules or to register to be a "Lucky Fan" go to www.trivalleytv.org and click on the "Lucky Fan" icon located on that page.
Read the full story here Web Link posted Sunday, September 22, 2013, 7:41 AM
Comments (4)
a resident of Another Pleasanton neighborhood
on Sep 23, 2013 at 2:01 pm
I just saw the drawing, so I quickly went to the site and filled out all the lines. THEN, right before clicking, I saw the line, winner will be posted on Facebook! UGH. I had to delete all my info. I am not ON Facebook, and will never be. I am a long time resident. Wish I could 'play' too. Such discrimination. I opt out of all companies who 'use' facebook, interact with them. Does it come with 'privacy protection' guarantees and insurance???
a resident of Livermore
on Sep 23, 2013 at 3:06 pm
The truth will eventually trickle out. I'm not going to sign up because I also believe that Facebook attracts lots of human waste waiting to sucker punch the public.
a resident of Downtown
on Sep 24, 2013 at 12:47 pm
First, TV30 Lucky Fans are notified by e-mail that they have won. Their first name and City only are announced on TV30 and on Facebook.
a resident of Downtown
on Sep 24, 2013 at 12:49 pm
First, TV30 Lucky Fan winners are notified that they have won via e-mail. The winner's first name and City only are announced on TV30 as well on the station's Facebook page.
Don't miss out on the discussion!
Sign up to be notified of new comments on this topic.
Editorial: Pleasanton Police Department's unwarranted silence unacceptable
By Gina Channell-Allen | 17 comments | 1,327 views
Doubling Dublin
By Tim Hunt | 2 comments | 605 views
The Cleric and the clerk*
By Tom Cushing | 12 comments | 463 views
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text: Amazon has found pretty significant success with some of its original shows -- particularly the Golden Globe- and Emmy-winning Transparent -- and it seems the company's TV ambitions don't stop with on-demand programming. According to Bloomberg, Amazon is considering an online TV service that broadcasts live and includes programming from major players like CBS and NBC. It would be the latest service to directly challenge the dominance of traditional cable providers like Comcast and join competitive options like Sony's PlayStation Vue and Dish's Sling TV. Both of those services offer a host of live content that has been typically tied to those with a cable subscription.


text:
Reviews - The White Swan4
ROTHER STREET, Stratford upon avon, CV37 6NH
Price FromView Rooms
Customer Reviews
Our reviews are from genuine LateRooms.com customers. We verify that the customer has stayed before leaving a review.
Very Good
98% would recommend it to a friend
Customer service
Food quality
Room quality
We enjoyed our stay, beautiful building , food was excellent
Can't fault the place rooms are amazing ..location ideal
The White Swan
Loved the White Swan.
The room was beautiful and we really enjoyed the bath. The only change I would make is to invest in some quality linen and a king single rather than a single bed. For an adult this was rather small!
Read More Less
Stunning hotel with excellent service
Beautifully restored, all of the rooms and spaces of the hotel nod to quality, relaxation and care - a lot of thought has gone into it.
The welcome on arrival was wonderful and every member of staff we met had a smile on their faces. Cannot recommend highly enough.
Read More Less
A happy discovery
Friendly but efficient service.
Got the impression that it's a happy place to work. It's a beautiful building with wonderful historical features and it is very handy for the centre of town, river and theatre. We will return.
Read More Less
What a great hotel fantastic breakfast
Only negative was the parking.
It cost me an additional 14 pounds for overnight parking and bearing in mind I had paid 130 for the room I thought that the charge should be included in the room. apart from that the whole stay was great. The hotel is in a great position to explore the town.
Read More Less
Surprised by the room and the hotel itself.
For sure looked after and all the staff are provide of the place can be see by the cleanliness and service. Breakfast was brilliant
Read More Less
Lovely quaint hotel, amazing and helpful staff.
Breakfast was great. Location is ideal to most things Stratford has to offer
Read More Less
Excellent hotel, lovely stay
We decided at the last minute to extend our holiday by staying in Stratford Upon Avon and used the late rooms site to find us a hotel for Sunday night.
For £99 we chose a classic double room (with breakfast included) and although it was small, the character, cleanliness and general high level of the hotel were excellent. We ate in the restaurant and the food was very good, to a good a la carte standard. The staff were very friendly, welcoming and attentive. We wouldn't hesitate in coming back to this lovely, well situated hotel.
Read More Less
What a lovely place to stay!
Excellent place to stay very good food, beautiful room, staff helpful.
Hotel in a good spot just walk out the door and you are there among the shakesphere history.
Read More Less


text:
Fictitious portrait called King Stephen
4 of 20 portraits of King Stephen
© National Portrait Gallery, London
Fictitious portrait called King Stephen
after Renold or Reginold Elstrack (Elstracke)
line engraving, (1618)
3 1/4 in. x 1 3/4 in. (81 mm x 45 mm) paper size
Purchased with help from the Friends of the National Libraries and the Pilgrim Trust, 1966
Reference Collection
Click on the links below to find out more:
Sitterback to top
- King Stephen (circa 1092-1154), Reigned 1135-54. Sitter associated with 20 portraits.
Artistback to top
- Renold or Reginold Elstrack (Elstracke) (1570-in or after 1625), Engraver. Artist associated with 149 portraits.
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Subjects & Themesback to top
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Can you tell us more about this portrait? Spotted an error, something missing, or do you know anything that we don't know? If you have any information to share please complete the form below
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Parishes: Boughton Monchensie
The History and Topographical Survey of the County of Kent: Volume 5
Parishes Boughton Monchensie BOUGHTON MONCHENSIE LIES the next ... Boughton. It has the addition of Monchensie, (commonly pronounced Monchelsea) ... in the neighbourhood of it, Boughton...
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Sheet 038
Map of Cheshire
Blacon, Boughton Heath, Chester, Christleton, Handbridge, Hoole, Littleton, Mickle Trafford, Mollington, Piper's Ash, Saltney, Saughall, Sealand, Upton, Upton Heath, CH1 5, CH3 5, CH1 2, CH3 7, CH4...
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Sheet 015
Map of Flintshire
Balderton, Boughton Heath, Bruera, Christleton, Cuckoo's Nest, Dodleston, Eaton Hall, Eccleston, Handbridge, Rowton, Saighton, Saltney, CH4 9, CH3 5, CH3 6, CH3 7, CH4 7, CH4 8
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Sheet 053
Map of Kent
Boughton Malherbe, Chart Sutton, Egerton Forstal, Farthing Green, Five Wents, Grafty Green, Hawkenbury, Kingswood, Leadingcross Green, Liverton Street, Platt's Heath, Pye Corner, Sandway, Sutton Valence, Tong,
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OS Map name 038/16
Map of Chester and its Environs
Boughton Heath, Christleton, CH3 5, CH3 7
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House of Lords Journal Volume 4: 1 June 1641
Journal of the House of Lords: Volume 4, 1629-42
Witnesses about Boughton and Williams. Ordered, That a Commission ... in Military Affairs of William Boughton, Esquire, One of His Majesty's Justices ... of such Witnesses as they the said Wm Boughton...
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Parishes: Newbold-on-Avon
A History of the County of Warwick: Volume 6, Knightlow Hundred
Heath were inclosed under an Act of 1773. 11 Manors NEWBOLD, assessed ... by William Boughton 30 and presumably descended with his other estates ... Lawford, by Edward Boughton in 1542. 34 It is...
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Parishes: Bilton
A History of the County of Warwick: Volume 6, Knightlow Hundred
is said to have been built by Edward Boughton of Lawford; ... shield bearing the arms of Boughton with supporters. The finials ... Boughton (who already held the portion of Bilton that had...
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Parishes: Linton
The History and Topographical Survey of the County of Kent: Volume 4
of Cocks heath, lies the parish of Linton, antiently written LYLLYNGTON, ... to Cocks-heath, upon the ridge of quarry hills, the summit of which is the northern ... that district, only a small part of the...
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The History and Topographical Survey of the County of Kent: Volume 7
Manor and Buckwell, in Boughton Aluph, 390, 392. Austen, Sir Robert, heirs ... Susan, 554; Barrow's, of Hinxhill, 563, 564, 565; of Boughton Aluph, 394. ... Bartelot's, account of, 501, 510. Barton Manor, in...
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view all 17 photos
view all 40 songs
view all 6 albums
New original album by DeDe (with Andrew Fromm, Kevin DeClue, & Mick Walsh) coming late OCTOBER 2015 - "DREAM of YOU" stay tuned!
2015 Song of the Year.com Finalist- Prime of Life
2015 IMEA Song of the Year Nominations: Prime & Stronger
2014 IMEA AC ALBUM of the YEAR: Love & Fairy Tales
2014 IMEA HOLIDAY ALBUM of the YEAR: World of Christmas
2014 Dallas Songwriting Contest: Overall 2nd Place 2 in Categories
2013 & 2014 UK Songwriting Contest FINALIST: 2 songs: "Plans" & "Blame It"
International Songwriting Contest 2014 FINALIST: The Fairy Tale Song
Breathecast.com's Timothy Yap says, "Wedekind brings something fresh from each of these songs...some of the most intricate pieces from the American Songbook. When it comes to song interpretation, Wedekind is without peer."
DeDe has 3 albums including: WORLD of CHRISTMAS, produced by Grammy-Winner, Jeff Bova. Bova says, "World of Christmas is an album that will not only touch your heart but also your soul".
more bios
DeDe's new album is releasing OCTOBER 2015- Dream of You. Also getting my concert dates and social media, website updated with my House of Blues New Orleans show August 27, and Light @ Lighthouse San Pedro CA Sept. 5....Monthly News & monthly Music BLOG! Also rehearsing with DeDe & the Dreams band!
Recording in LA with Charlie Midnight next week - in August.
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broadjam.com © 2015


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- Age / Gender:
- 21, Male
- Location:
- Tulsa, OK
- Joined:
- 12/25/09
- All Stats >
Follow me on Twitter! @TacoSoupNG Check out the Channel! Youtube.com/soupsqua dNG Look us up on Soup Squad!
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Ranked as Police Sergeant
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- Krutchesdk
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Latest Movies
Ramen I miss You Rated Stars Ramen, I miss talking shit with you. Comedy - Parody Strawberry Desert Rated Stars strawberry forgets that he's the LAST FOOD EVER! Comedy - Original Clock Day's Big Hitters Rated Stars Three Tough Dudes, One Big Movie Experimental Plan 9 from Outer Sauce Rated Stars Ed Wood directs a movie! Comedy - Original
Recent Game Medals
SECRET MEDAL 10 Points Unlock this medal to see it's details. Medal Stats.
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100 Percent 100 Points 100 (one hundred) (Roman numeral C, reinforced by Latin centum) is the natural number following 99 and preceding 101. Medal Stats.
Secret #36 5 Points A soft drink (also called pop, soda, coke, soda pop, fizzy drink, tonic, mineral or carbonated beverage) is a beverage that typically contains water (often, but not always carbonated water), a sweetener, and a flavoring agent. Medal Stats.
Secret #5 5 Points A mobile phone (also known as a cellular phone, cell phone and a hand phone) is a device that can make and receive telephone calls over a radio link whilst moving around a wide geographic area. Medal Stats.
Secret #25 5 Points The incandescent light bulb, incandescent lamp or incandescent light globe is an electric light which produces light with a filament wire heated to a high temperature by an electric current through it, until it glows. Medal Stats.
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Total Medals Earned: 691 (From 130 different games.)
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Apocalypse: Survive it Rated Stars Survive the apocalypse! Oh, by the way; you are blind! Made for GameJolt New Years' Jam. Adventure - Other SS - Taco Dressup Rated Stars Dress up taco and fuck Gadgets - Dress Up SS - Carrot Dressup Rated Stars Dress up carrot and fuck Gadgets - Dress Up [Soup] The Search Rated Stars Search for the Disciple of Kocksoppa in this action-thriller. Action - Other
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Lead and Shrooms Rated 3.9688 Stars A psychedelic, blood soaked adventure in the forest. Action American Alfer Rated 4.1226 Stars I Enjoy Dissecting Girls. Music Video Rabbits Rated 3.8685 Stars DeadFish shows us one of his many independent films. Comedy - Original Stone N' Deadfish II Rated 3.6467 Stars another soupy short? Comedy - Original
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Kingdom Rush Rated 4.4999 Stars The kingdom is under attack! Defend your realm against the hordes of evil! Strategy - Tower Defense Frontier Rated 4.4415 Stars Make your own fortune in this sprawling Oregon Trail style RPG/Trading game Adventure - RPG The Golden Bird Rated 2.3497 Stars A modern tale of an unusual boy in search of a golden bird who stole the banker's apples. Puzzles - Other The Dead Case Rated 4.3347 Stars Solve the mystery of the ghosts Adventure - Point 'n Click
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