text:
Our list of the best smartphones available to buy with a budget of £100
A couple of years ago you had to fork out a significant amount to get your hands on a phone with a decent display, good performance and the ability to browse the internet. But the price of smartphones has decreased rapidly in recent times, meaning there are some true bargains to be had.
We've picked out the best smartphones that are priced under £100 (SIM-Free or on Pay As You Go). You'll be pretty amazed at what you can get your for your money. All the mobiles listed are available to buy in the UK and we'll continue to update our list throughout 2015.
Anyway, enough talk and on with our list of the best smartphones available for under £100.
All of these phones are cheap, but at just £60 the Nokia Lumia 530 is almost unbelievably affordable. For that money you get a 4.0 inch 480 x 854 display with a pixel density of 245 pixels per inch for clear visuals, a 1.2GHz quad-core Snapdragon 200 processor and 512MB of RAM, so it's a surprisingly well specced device.
It's solidly built too, with a colourful plastic shell which you can swap out if you ever feel like a change. There's no 4G, just a 5 megapixel camera and only 4GB of storage, but there's a microSD card slot too so you can easily expand on that and it runs Windows Phone 8.1 so you can get brand new features like Cortana and Action Centre without having to spend much money.
The original Motorola Moto E was incredible value and sold well as a result. Now Motorola's back with a slightly better and marginally more expensive successor.
At just £90 it's still incredibly affordable but it's surprisingly good too, with a 4.5-inch 540 x 960 screen for a pixel density of 245 pixels per inch, a 1.2GHz quad-core Snapdragon 410 processor and 1GB of RAM.
It also has a fairly attractive, albeit plastic design and feels solidly built. Plus it's water resistant so it can take a splash and while it only has 8GB of built in storage there's also a microSD card slot.
The 5 megapixel camera is pretty poor and it lacks advanced features like NFC, but it's a reliable smartphone with solid performance for under £100 and it even has 4G, which still isn't standard at this sort of price.
The Nokia Lumia 635 is essentially a tweaked version of the Nokia Lumia 630, adding 4G connectivity and changing from a matte plastic cover to a more eye-catching glossy one.
Other than that it's the same as that already impressive handset, with a clear 4.5-inch 480 x 854 IPS LCD display, a 1.2GHz quad-core Snapdragon 400 processor, 512MB of RAM, 8GB of storage, a microSD card slot, an 1830 mAh battery and a 5 megapixel camera.
That's combined with the lightweight and sleek Windows Phone 8.1 operating system that leaves it with slick performance and you can get all of this for just £85, or if you can live without 4G you could always opt for the Lumia 630 and save yourself £15.
The Sony Xperia E1 doesn't do much to stand out. It's a plastic block which has none of the premium design features found on most Xperia handsets. But it's solidly built and with just a 4.0 inch screen it's one of the most pocket-friendly smartphones around.
That's not the only reason it's worth considering though, as it also has a 100Db speaker for loud, clear audio quality and a dedicated Walkman button so you're never far from your music.
Under the hood there's a 1.2GHz dual-core Snapdragon 200 processor and 512MB of RAM, so it should be up to the task of carrying out anything you could expect from a smartphone.
With a 480 x 800 display it manages a reasonable pixel density of 233 pixels per inch too and while it only has a 3.2 megapixel camera Sony has included a whole bunch of modes and filters to play with, so it's easy to get creative with your photography.
It might not be the most exciting phone on the planet but it covers the basics, delivers a music-centric experience and will fit in even the smallest of pockets, all for just around £55.
The EE Harrier Mini can be seen as the successor to the EE Kestrel, along with the pricier EE Harrier.
For under £100 it gives you a sharp 4.7-inch 720 x 1280 display, a 1.2GHz quad-core processor, 1GB of RAM and a surprisingly high spec 8 megapixel camera.
Being an EE phone it also supports 4G of course, so your mobile data needs are covered and with Android 5.0 its software is up to date.
Perhaps most excitingly it's currently the cheapest phone to offer WiFi Calling, giving it a serious selling point, particularly as only a handful of other handsets have the feature at all.
With solid battery life, a microSD card slot and a decent if unexceptional build there's not much that's missing here, especially not for such a low price.
The original Moto G with 4G is one of the best budget smartphones on the planet. With starting prices of £110 it comes in at just over £100 but it often pops up on deals for around £100, so keep your eyes peeled.
In any case it's well worth the extra, as with a 1.2GHz quad-core Snapdragon 400 processor and 1GB of RAM it's a snappy performer, while its 4.5 inch 720 x 1280 IPS LCD display is both big and high quality as low end phones go, with a pixel density of 326 pixels per inch.
There are other good things to say about it too. It runs the latest version of Android, has a capable 5 megapixel camera and an attractive build and with 8GB of storage (or more if you pay more) it's not as limited as some cheap phones.
It's since been superseded by a 2nd generation Moto G with a larger screen and that's a great option too but it will stretch your budget even further.
This is a name you might not know, but you should, as this Huawei handset is fantastic value for money.
It comes with a big and surprisingly high resolution 5.0-inch 720 x 1280 display, a 1.3GHz quad-core processor, 1GB of RAM and a generous 16GB of built in storage, plus a microSD card slot.
But the camera is the real star here, as its 8 megapixel snapper is capable of taking detailed shots and it even has a solid 2 megapixel camera on the front for taking selfies.
The design is bland and Huawei's Emotion UI is a bit simplistic for our tastes, but with a big screen, a good camera and a decent amount of power it's well worth what it costs.
The long line of high quality yet affordable Lumia's has arguably reached its peak with the Lumia 640.
The phone squeaks in at just over £100, but you can expect to find it on pay as you go for less and you get an awful lot for your money. Its large 5.0-inch 720 x 1280 display is hard to ignore, while its 1.2GHz quad-core Snapdragon 400 processor and 1GB of RAM ensure it's not short on power, plus it has a capable 8 megapixel camera and runs Windows Phone 8.1.
It's not likely to become dated any time soon as it will be upgraded to Windows 10 for phones when that launches and on top of the phone itself you also get a one year subscription to Office 365 when you buy it.
That's valued at £59.99 in itself and includes 1TB of OneDrive storage, so even though there's only 8GB of built in memory there's no reason to run short.
Not content with just providing SIM cards and contracts, Vodafone also makes own-brand handsets and the Smart Prime 6 is one of its best yet, combining an affordable price tag with surprisingly impressive specs.
The highlight is arguably its impressive battery life, as while Vodafone hasn't confirmed how big its juice pack is it's rated for up to 18 hours of talk time. That's well above average for any smartphone, let alone a budget one, so it's a great choice for power users.
It also has a 1.2GHz quad-core processor and 1GB of RAM so it's fairly powerful and it has a large 5.0-inch 720p screen. Its 8 megapixel camera is seriously impressive for the price too and while it only has 8GB of storage it also has a microSD card slot with support for cards of up to 64GB. It even supports 4G for speedy mobile data.
The Vodafone Smart Prime 6 is surprisingly stylish too, with a slim 9mm thick build and metal keys, though the casing is still plastic.< Back
All content and images are copyright © 2015 3G.co.uk all rights reserved


text:
Jobs Companies & Reviews Companies Salaries Interviews
Hiring? Post a Job
Travel Account Manager Interview Questions
Sort: Popular Date
Sort: Popular Date
Did you mean companies matching "Travel Account Manager"? See Companies
Jan 6, 2014
"Background check is extremely intrusive. They even asked me questions about my husband which I didn't really feel was appropriate."
1–1 of 1 Interview Question

subset: commoncrawl


text:
Coming up in the next {{countdown}} {{countdownlbl}}
Coming up next:
Skip to this video now
Now Playing:
Play Video
This transcript has been automatically generated and may not be 100% accurate.
Johnny Cash's Son Authors Children's Book
John Carter Cash announces new book and the release of the Johnny Cash stamp.
Rosanne Cash on Her Father
She speaks about life with her father, Johnny Cash.
Now Playing: Johnny Cash's Son Authors Children's Book
Now Playing: Rosanne Cash on Her Father
Now Playing: Johnny Cash's Son Reveals Father's Personal Life
Now Playing: 7/10/1985: Consumers Say No to 'New Coke'
Now Playing: Volkswagen Executives Apologize After Scandal
Now Playing: Controversy Ignites As SeaWorld Tries To Build Bigger Tanks for Its Killer Whales
Now Playing: VW America CEO Grilled on Capitol Hill
Now Playing: Deals and Steals: Fall Fashion
Now Playing: Jessica Mendoza Ignores Sexist Twitter Attack, Accepts Radio Host's Apology
Now Playing: Woman Faces Criminal Charges for Allegedly Shooting at Suspected Shoplifters
Now Playing: Auto Strike Deadline Looms for Fiat-Chrysler
Now Playing: NBA Legend Jalen Rose Describes His Remarkable Life Story
Now Playing: Texas Mom Sparks Publisher to Revise Textbook
Now Playing: How to Save Money on Dental Procedures
Now Playing: New York's Attorney General Launches Fantasy Sports Investigation
Now Playing: Powerball Jackpot Winner Quits Job
Now Playing: McDonald's All-Day Breakfast Menu Is Here
Now Playing: General Mills Recalls 1.8 Million Boxes of Cheerios
Now Playing: Newly Released Tapes Reveal Visionary Genius of Apple's Steve Jobs
Now Playing: Fantasy Sports Employee Scores Big on Rival Site
Now Playing: Shop to End Poverty
Now Playing: Forbes '30 Under 30' Summit
Now Playing: {{itm.title}}


text:
This session is designed to help you understand some of the inter-workings of the MHC IRB:
(1) How the IRB determines if a research project is human subject research vs. non-human subject research
(2) When is informed consent required for participation in a study or when can it be waived.
(3) How the IRB determines if a new research projects qualifies for expedited or full board review
In this brown bag session we will Review the AAHRPP standards and required elements for Clinical Trials Agreements. We will also explore the responsibilities of MHC HRPP,-MCRI Contract and Budget Specialist, and the researcher with regards to the executed CTA.
A breach of privacy or confidentiality violates an individual's rights and poses a risk of dignitary harm to the research participant, ranging from social embarrassment and shame, to stigmatization, and even damage to social and economic status. This session will discuss concepts of privacy vs. confidentiality, HIPAA privacy rule as it relates to research and how to protect the research subject's privacy and confidentiality.
Humanitarian Use Devices (HUD) and emergency use of investigational drugs prior to IRB approval are intended for a distinct population of subjects under specified circumstances. This session will help you to understand and know:
(1) How a medical device qualifies for HUD designation.
(2) What are the criteria for using a HUD?
(3) MHC IRB notification requirements for initial and continuing approval
(4) HRPP policy on emergency use of investigational drugs and devices
***Registration and / or access information will be sent out prior to each session. Please call the HRPP office at (810) 342-1003 if you have any questions.
The human subject regulations provide the authority to review, approve, require modifications in (to secure approval) or disapprove all research covered by those regulations [45 CFR 46.109(a); 21 CFR 56.109(a)]. While there are criteria for the approval of research (45 CFR 46.111; 21 CFR 56.111), the regulations do not provide direction on the quality of the review. This session will discuss key items that are related to the quality of the review of a protocol by the convened IRB.
One of the most problematic areas for IRBs, and a common finding in ORHP Compliance Letters, is that IRBs approve research with conditions that are not minor. The regulations do not explicitly address IRB approval with conditions, the Office of Human Research Protections allows for such IRB decisions. This session will help establish the difference between what is a minor condition from what is a substantive condition.
IRBs are known to spend a lot of time dissecting, revising and rewording consent forms, and making sure that the required elements of consent are all adequately covered in the document. The required and additional elements of consent appear in 45 CFR 46.116(a) and (b). This session will discuss the preface to those requirements, which sometimes takes a back seat, but is just as important as the consent requirements.
This session will discuss the IRB's responsibility to assess the scientific validity of proposed research. This charge is related to the regulatory determinations required for approval of research and to the IRB's ethical responsibilities to protect subjects.


text:
Gifts by Price
Gifts by Recipient
Gifts by Occasion
Featured Gifts
Daily Sales Up to 70% OFF Limited-time deals start every day at 12 pm ET.
Free Shipping on orders over $49.00
Product Information:
Apply microfiber feather technology to every hard-to-reach dusting job with the softest touch and toughest dust-grabber in the industry. Conveniently sized for home and office use. Tiny microfiber hooks grab and hold dust particles without damaging sensitive surfaces. Dusts without chemicals. Reduces bacteria and allergens. Hand-washable, allowing for hundreds more uses than a disposable product.
Weights & Dimensions
|Item: Dusters
|Country of Manufacture: China
About the ManufacturerUNISAN offers an array of products that we are pleased to offer to you!
More About This Product
Free Shipping on orders over $49.00
Ships to:
Ships via: FedEx/UPS
Enter your shipping zip or postal code to calculate shipping estimatesGet Delivery Estimate
We want you to love it. If you don't, you may return your purchase within 30 days of delivery. All you need to do is visit our Self-Service Return Center for instructions. Return credits are issued within 24 hours once the item is received and inspected. Please note that shipping charges are non-refundable. The cost of return shipping will be deducted from your refund. 30-Day Return Policy
Tell Us What You Think!
Tell us what you think about this product. It will help us provide you with better products in the future and help your fellow shoppers make better purchases.Write the first review


text:
New Roles for Earth Science in Climate Policy - Feature - January 12, 2009
New Roles for Earth Science in Climate Policy
January 12, 2009
In a new issue brief, "Climate Change and Policy Considerations: New Roles for Earth Science," RFF Senior Fellow Molly Macauley outlines the important role observations taken from space may have in designing global climate policy.
Macauley says that as policymakers work to design new strategies and metrics, Earth observation should receive serious consideration. She identifies a range of areas where data collected from space could play an important role, including:
Climate Change Policy and Considerations: New Roles for Earth Science
Molly Macauley
January 2009
- Quantifying CO2e (thus, greenhouse gases including CO2, N2O, CH4, HFCs, PFCs, SF6)
- Observing other climate variables and proxies for them (e.g., land use, tree cover, freshwater quality and quantity, and ecosystem status)
- Contributing to understanding effects of many types of mitigation policy
- Informing assessment of overall effects (costs) to the economy, various sectors (e.g. electricity, transport, agriculture), demographic groups, and managers of environmental and other non-marketed natural resources
- Observing unintended effects (e.g. reallocation of land to agricultural production and its effects on carbon sequestration)
- Assessing effects of offsets
- Assessing effects of possible banking regimes (by consistently observing policy effects over time)


text:
Search the Collection
Artist associated with 1 portrait
after C. Bond
C. Bond
Artist associated with 1 portrait
Thomas Pitt, 2nd Baron Camelford
after C. Bond
mixed-method engraving, 1804 or after
Tell us moreback to top
Can you tell us more about this person? Spotted an error, something missing, or do you know anything that we don't know? If you have any information to share please complete the form below


text:
- Hot Animes This Season
- Overlord,
- Charlotte,
- Gangsta,
- Gate,
- Rokka no Yuusha,
- God Eater,
- Monster Musume,
- Durarara!X2 Ten,
- Akagami no Shirayuki
Dragon Drive Episode 18
Watch Dragon Drive Anime Episode 18 for free with English Subbed. View Dragon Drive Episode 18 Online.
if Dragon Drive – Episode 18 are not working, please try one of the Video Mirrors.
Upload by: gerard
Updated: November 13, 2012
You are watching Dragon Drive Episode 18 in High Quality English subbed. Dragon Drive Episode 18 is from Dragon Drive anime series. If you enjoyed Dragon Drive Episode 18 please share it with others! Please note that this Episode is hosted on a third-party video sharing website, and it is not affiliated with Chia-Anime.
Anime › Dragon Drive › Episode 18


text:
WEDNESDAY, Aug. 8 (HealthDay News) -- Older people who engage in balance and strength training can significantly reduce their risk of falling, a new study suggests.
Researchers from Australia found that incorporating these exercises into routine activities reduced falls among the elderly by nearly one-third. However, less than 10 percent of older people regularly participate in strength training and even fewer exercise to improve their balance, according to study author Lindy Clemson, a professor in occupational therapy and aging at the University of Sydney, and colleagues.
Their study involved more than 300 men and women aged 70 or older, all of whom had fallen at least twice or injured themselves in a fall in the preceding year. The researchers assigned the participants to one of three exercise programs.
One group followed what the researchers called the "Lifestyle-integrated Functional Exercise" program. This program incorporated balance and lower limb strength training into the participants' daily routines. By following this program, the older people worked on their strength and balance while they walked, stepped over objects and moved from sitting to standing.
The second group was given a structured exercise program to complete three times a week using ankle cuff weights. The third group, the "control" group, was assigned gentle "sham" exercises.
All of the groups recorded any falls they had over the course of one year. The researchers also measured their static and dynamic balance, ankle, knee and hip strength, and examined their daily living activities and quality of life.
Clemson and colleagues estimated that the lifestyle-integrated exercise group had a 31 percent reduction in rate of falls, compared to the control group. The lifestyle-integrated exercise participants also improved both static and dynamic balance and ankle strength. Moreover, the study showed they had enhanced function and
|Copyright©2012 ScoutNews,LLC.
All rights reserved


text:
The Style Gallery is a great new way for you to view, love, and share outfit photos. It's meant to showcase
all the expressive, creative, and inspirational personalities of our community!
Love you too!
Your email has been sent!
Show you care about the environment by carrying all your consumables around in this reusable, recycled bag! Woven from 95% post-consumer material, this sunny yellow sack displays an eco-eager beaver on both front and back, resting against a backdrop of slightly gnawed trees. Are you also ready for a snack? Stuff your bento box or sandwich bag in this neon-handled tote when you head off to work, or use it to bring books back from the library, and you'll be logged into an engagingly eco-friendly look!
Sorry this item isn't available anymore
Remarkable fashion & decor, inspired by our community, & delivered with uncommon care.
Copyright ©2002-2015 ModCloth.com. All Rights Reserved.
Site Map
CA Notice
Privacy & Security
Terms of Use


text:
Page 1
Regulation of Bovine Tumor Necrosis Factor-?-Induced
Protein 6 in Ovarian Follicles during the Ovulatory
Process and Promoter Activation in Granulosa Cells
Khampoune Sayasith, Nadine Bouchard, Monique Dore ´, and Jean Sirois
Centre de Recherche en Reproduction Animale and the De ´partement de Biome ´decine Ve ´te ´rinaire (K.S., N.B., J.S.)
and De ´partement de Pathologie and Microbiologie (M.D.), Faculte ´ de Me ´decine Ve ´te ´rinaire, Universite ´ de Montre ´al,
Saint-Hyacinthe, Que ´bec, Canada J2S 7C6
To study the regulation of bovine TNF?-induced protein 6
(TNFAIP6) prior to ovulation, preovulatory follicles obtained
after the treatment with human chorionic gonadotropin
(hCG) were used. RT-PCR analyses showed that levels of
TNFAIP6 mRNA were low before hCG but significantly in-
creasedafter hCG treatment in follicles. Further analyses and
immunohistochemistry indicated that this increase in tran-
script and protein levels occurred in theca and granulosa cells.
To investigate molecular mechanisms involved in TNFAIP6
studied in granulosa cell cultures. Mutant studies identified
the minimal region conferring full-length promoter activity,
in which activator protein-1 (AP1) and cAMP response ele-
ment (CRE) elements were required for promoter activity.
Overexpression of dominant-negative AP1 and activating
transcription factor/cAMP response element-binding protein
(CREB) inhibited forskolin-inducible promoter activity. DNA
binding assays demonstrated the importance of AP1 and CRE
the CRE complex. Chromatin immunoprecipitation assays con-
moter. Treatment with forskolin, prostaglandin E2, and cata-
lytic subunit protein kinase (cPKA) stimulated, but H89, PKA
inhibitor peptide, and indomethacin inhibited, TNFAIP6 pro-
moter activity and gene expression in granulosa cells. Collec-
tively, this study is the first to describe that the ovulatory pro-
cess in cows is associated with a gonadotropin-dependent
induction of TNFAIP6 in ovarian follicles and provide the mo-
lecular basis through which AP1 and CRE sites and PKA acti-
vation played important roles in the regulation of TNFAIP6 in
granulosa cells. (Endocrinology 149: 6213–6225, 2008)
hyaladherin superfamily of hyaluronan (HA)-binding pro-
teins (1). Its amino acid sequence consists of two contiguous
modules: N-terminal link and C-terminal complement pro-
tein subcomponents C1r/C1s, urchin embryonic growth fac-
tor, and bone morphogenetic protein 1 modules. The link
module is known to interact with several ligands, including
HA, serum-derived factor inter-?-trypsin inhibitor, aggre-
roles of the C1r/C1s, urchin embryonic growth factor, and
bone morphogenetic protein 1 module remain unclear (2).
Expression of TNFAIP6 is usually undetectable in normal
NF-?-INDUCED PROTEIN 6 (TNFAIP6) is an approx-
imately 35-kDa secreted protein that belongs to the
tissues but rapidly induced during inflammatory diseases
(3). Indeed, TNFAIP6 is rapidly activated by stimuli, such as
TNF-?, IL-1, and lipopolysaccharide in several cell types,
including fibroblasts, peripheral blood mononuclear cells,
chondrocytes, and synovial cells (3), whereas high levels of
of rheumatoid arthritis patients (4), in which its potent an-
tiinflammatory and chondroprotective roles in arthritis have
been suggested (2).
Ovulation is a complex process that involves a series of
biochemical and biophysical events ultimately leading to
the rupture of the preovulatory follicles and the release
of the oocyte. This process shares numerous signs of an
acute inflammatory reaction, including hyperemia, edema,
emigration of leukocytes, and induction of proteolytic
and collagenolytic activities (5–7). The preovulatory surge
of LH/human chorionic gonadotropin (hCG) triggers
the ovulatory process during which the expression of
TNFAIP6 is highly induced in follicular cells of rodents
and a mare (8–10). During ovulation, a marked production
of the extracellular matrix (ECM) surround the cumulus
cells was observed, leading to the characteristic expansion
of the cumulus-oocyte complex (COC) (11, 12). Several
studies have documented that TNFAIP6 plays an impor-
tant role at this level interacting with inter-?-trypsin
inhibitor and HA within the ECM, and mice lacking
TNFAIP6 gene have severely impaired cumulus expan-
sion, and are infertile (8, 13–17).
First Published Online August 7, 2008
Abbreviations: A-CREB, Dominant-negative CREB; A-FOS, domi-
nant-negative AP1; AP1, activator protein-1; AREG, ampiregulin;
C/EBP, CCAAT/enhancer-binding protein; ChIP, chromatin immuno-
precipitation; COC, cumulus-oocyte complex; cPKA, catalytic subunit
protein kinase; CRE, cAMP response element; CREB, cAMP response
element-binding protein; ECM, extracellular matrix; EGF, epidermal
growth factor; EREG, epiregulin; FSK, forskolin; GAPDH, glyceralde-
hydes-3-phosphate dehydrogenase; HA, hyaluronan; hCG, human cho-
rionic gonadotropin; INDO, indomethacin; PD, PD98059; PGE2, pros-
taglandin E2; PKA, protein kinase A; PKI, PKA inhibitor peptide; PMA,
SB20358; SDS, sodium dodecyl sulfate; STAT, signal transducer and
activator of transcription; TNFAIP6, TNF?-induced protein 6.
Endocrinology is published monthly by The Endocrine Society (http://
www.endo-society.org), the foremost professional society serving the
endocrine community.
Printed in U.S.A.
Endocrinology 149(12):6213–6225
Copyright © 2008 by The Endocrine Society
doi: 10.1210/en.2008-0459
Page 2
There are only a few studies on the molecular control of
TNFAIP6 induction, showing that the cis-acting elements
CCAAT/enhancer-binding protein (C/EBP) and activator
transcriptional activation in fibroblasts induced by cytokines
(18, 19). However, it is known that the relative importance of
elements for such a gene promoter varies greatly, depending
descriptive studies on TNFAIP6 in preovulatory follicles
during ovulation (8–10), and no attempt has been done to
unravel the molecular basis for the regulation of TNFAIP6
promoter in follicular cells of any species, including rodents,
horses, and cattle, leaving the mechanisms of TNFAIP6 stim-
ulation by gonadotropins unclear. Therefore, the objectives
of the study were to investigate the regulation of TNFAIP6
induced by gonadotropins in bovine follicles in vivo and to
characterize the molecular mechanisms involved in the bovine
TNFAIP6 promoter activation in vitro. Such studies are impor-
tant because they will likely be relevant to humans, a species
with a long ovulatory process also (36 h after hCG) (20).
Materials and Methods
??32P?dCTP was purchased from PerkinElmer Life Sciences (Wood-
bridge, Ontario, Canada). Lipofectamine Plus reagent, TRIzol total RNA
isolation reagent, a 1-kb DNA ladder, synthetic oligonucleotides, and
culture medium were obtained from Invitrogen Life Technologies (Bur-
lington, Ontario, Canada). Fetal bovine serum was purchased from
Hyclone Laboratories (Logan, UT). The dual-luciferase reporter assay
Promega Corp. (Madison, WI). Hybond-P polyvinylidene difluoride
membranes, Rainbow molecular weight markers, the ECL Plus Western
blotting system, horseradish peroxidase-linked donkey antirabbit or
antigoat secondary antibody, and restriction enzymes were obtained
from Amersham Pharmacia Biotech (Baie D'Urfe ´, Que ´bec, Canada). All
polyclonal antibodies used in the study and protein A/G-Sepharose
beads were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz,
CA). Aprotinin, leupeptin, antipapain, benzamidine, chymotrypsin,
pepstatin, prostaglandin E2 (PGE2), and phorbol 12-myristate 13-acetate
(PMA) were purchased from Sigma-Aldrich (Oakville, Ontario, Can-
ada). Proteinase K, OneStep RT-PCR system, DNeasy tissue kit, and
QIAquick nucleotide removal kit were purchased from QIAGEN Inc.
(Mississauga, Ontario, Canada). Expand high-fidelity PCR system was
obtained from Roch Applied Science (Laval, Que ´bec). Forskolin, indo-
methacin, protein kinase A (PKA) inhibitor H89, p38 MAPK inhibitor
SB20358, and ERK1/2 inhibitor PD98059 were purchased from Calbio-
chem (San Diego, CA). QuikChange site-directed mutagenesis kit was
obtained from Stratagene (La Jolla, CA). Vectastain ABC kit was pur-
chased from Vector Laboratories (Burlingame, CA). Expression vectors
for the catalytic subunit of PKA (cPKA) and PKA inhibitor peptide (PKI)
were generously provided by Dr. Richard Maurer (Oregon Health and
Science University, Portland, OR), whereas those for dominant-negative
AP1 (A-FOS) and activating transcription factor/cAMP response ele-
ment-binding protein (CREB) (A-CREB) were provided by Dr. Charles
Vinson (National Cancer Institute, Bethesda, MD). Bovine preovulatory
follicle blocks were kindly given by Dr. Jacques Lussier (Biome ´decine
Ve ´te ´rinaire, Universite ´ de Montre ´al).
In vivo models of ovulation
Holstein heifers 2–3 yr of age exhibiting normal estrous cycles were
used as a model of hCG-induced ovulation to study the regulation of
TNFAIP6 during the ovulatory process in vivo, as previously described
(21). Briefly, bovine preovulatory follicles were obtained after induction
of luteolysis on d 7 of the estrous cycle and iv administration of an
ovulatory dose of hCG (3000 IU) 36 h after induction of luteolysis. The
ovary bearing the preovulatory follicle was isolated by ovariectomy (via
of time from hCG administration to ovulation is 26–28 h in this animal
model. The preovulatory follicles were dissected from the ovary with a
scalpel, and pieces of follicle wall (i.e. theca interna with attached gran-
ulosa cells) were prepared and further dissected into isolated prepara-
tions of granulosa and theca cells (21). All tissue samples were stored at
70 C. All animal procedures were approved by the Institutional Animal
Care and Use Committee of the Universite ´ de Montre ´al and were con-
sistent with the guidelines of the Canadian Council of Animal Care.
RNA extraction and RT-PCR analysis
All tissues were obtained from a slaughterhouse, and total RNA was
isolated from bovine preovulatory follicles, granulosa cell cultures, and
tissues using the TRIzol reagent and a Kinematica PT 1200C polytron
homogenizer (Fisher Scientific, Montre ´al, Canada), according to the
manufacturer's instructions. RT-PCR were performed using extracts
(100 ng), the OneStep RT-PCR kit as directed by the supplier (QIAGEN),
sense and antisense primers specific for bovine TNFAIP6, and glycer-
aldehydes-3-phosphate dehydrogenase (GAPDH; internal control)
genes, producing a 636- and 850-bp DNA fragments, respectively (sup-
plemental Table 1, published as supplemental data on The Endocrine
reaction conditions were one cycle of 50 C for 30 min and 95 C for 15 min
followed by 29 cycles for TNFAIP6 or 25 cycles for GAPDH of 94 C for
30 sec, 59 C for 1 min, and 72 C for 2 min. The number of cycles used
was optimized for each gene to fall within the linear range of PCR
amplification. PCR products were resolved on 2% Tris-acetate/EDTA-
agarose gels containing ethidium bromide (0.5 ?g/ml), visualized by
UV, and digitized. The digitized signals were quantified by densitom-
etry using the ImageQuant software (Amersham Pharmacia Biotech).
Isolation, deletion, and site-directed mutants of the bovine
TNFAIP6 promoter
A fragment of bovine TNFAIP6 5?-flanking region was isolated by
PCR using specific primers ?oligonucleotides 1 (sense) and 2 (antisense);
supplemental Table 1?, bovine genomic DNA extracts, and Expand
high-fidelity PCR system (Roch Applied Science). Primers were de-
signed from bovine TNFAIP6 gene sequence available from GenBank
of 40 cycles of 94 C for 30 sec, 58 C for 60 sec, and 72 C for 2 min, and
products were subcloned into pGEM-Teasy and sequenced. This ap-
proach provided an 1875-bp fragment of bovine TNFAIP6 genomic
DNA, which spans the region of ?1782 to ?93 (?1 ? A of the trans-
lational initiation codon ATG).
For deleted mutants, five fragments of bovine TNFAIP6, including
?1767, ?1047, ?575, ?200, and ?109 relative to the translational ini-
tiation site were generated by PCR amplification using six specific sense
primers (oligonucleotides 3, 4, 5, 6, 7, and 8, respectively, supplemental
Table 1) and one common antisense primer (oligonucleotide 9). Each
referred to as ?1767/?1TNFAIP6.LUC, ?1047/?1TNFAIP6.LUC, ?575/
Site-directed mutants for AP1, cAMP response element (CRE), signal
transducer and activator of transcription (STAT), C/EBPa, C/EBPb, and
C/EBPc elements,were produced
?1TNFAIP6.LUC as template, specific primers listed in Table 1, and the
QuikChange site-directed mutagenesis kit following the manufacturer's
protocol (Stratagene). Mutations were confirmed by DNA sequencing.
usingthe construct
Cell culture, transient transfections, and reporter
activity assays
Primary cultures of bovine granulosa cells, transient transfections,
and reporter activity assays were performed as previously described
(22). Briefly, cells freshly isolated from bovine follicles were seeded in
24-well plates at a density of 1–2 ? 105cells per 0.5 ml of MEM sup-
plemented with l-glutamine, nonessential amino acids, 2% fetal bovine
serum, insulin (1 ?g/ml), transferrin (5 ?g/ml), and penicillin (100
Endocrinology, December 2008, 149(12):6213–6225Sayasith et al. • Regulation of Bovine TNFAIP6
Page 3
U/ml)/streptomycin (100 ?g/ml) and incubated at 37 C in a humidified
atmosphere of 5% CO2. For transfection, confluent cell cultures were
structs using 2 ?l of LipofectAMINE (Invitrogen Life Technologies) in
0.3 ml of serum/antibiotic-free MEM, following the manufacturer's
protocol. Cotransfection with the SV40 Renilla luciferase control vector
(pRL.SV40; Promega) was performed to normalize results. A ratio of
experimental (TNFAIP6.LUC) to control vector (pRL.SV40) of 10:1 was
used. After 3 h of transfection, cells were incubated in 0.5 ml of fresh
MEM supplemented with 2% fetal bovine serum in the absence or
presence of forskolin (FSK; 10 ?m) or PGE2(50 ?m) for 24 h. After
incubation, cell lysates were prepared, and firefly and Renilla luciferase
activities were determined using the Promega dual luciferase assay
system and a Lumat LB 9507 luminometer (Berthold Technologies). In
some experiments, cotransfections were performed in the absence or
presence of cPKA or PKI expression construct (10 pmol), and cells were
incubated for 24 h in the absence or presence of PMA (100 nm), a protein
kinase A (PKA) inhibitor H89 (10 ?m), a p38 MAPK inhibitor SB20358
(SB; 10 ?m), ERK 1/2 inhibitor PD98059 (PD; 10 ?m), or indomethacin
(INDO; 10 ?m).
Granulosa cell nuclear extracts and EMSAs
(control) or presence of FSK (10 ?m) for 48 h, harvested, and submitted
to nuclear extraction as described (22, 23). Protein concentration was
determined by the method of Bradford (24) using the protein assay
(Bio-Rad, Hercules, CA). EMSAs were performed as previously de-
scribed (22, 23) with minor modifications. Briefly, an oligonucleotide
duplex (Table 1) depicted from TNFAIP6 promoter region ?575 to ?1
was end labeled with ??32P?dCTP and incubated in a binding buffer (20
?l) containing 15 mm Tris-HCl (pH 7.5), 1 mm EDTA, 100 mm KCl, 5 mm
MgCl2, 5 mm dithiothreitol, 12% (vol/vol) glycerol, 3 ?g of nuclear
protein extracts, and 1 ?g of poly(deoxyinosine-deoxycytosine) (Am-
ersham Biosciences). In competitive and supershift EMSAs, nuclear
extracts were preincubated for 20 min with unlabeled DNA compet-
itors or antiserum before the addition of other reagents. Binding
complexes were resolved by 5% acrylamide, 0.5? Tris-borate EDTA
gel electrophoresis.
Chromatin immunoprecipitation (ChIP)
ChIP assays were performed as described (23) with minor modifi-
cations. Briefly, granulosa cells were cultured in the presence of FSK (10
?m) for 24 h. After incubation, cells were incubated with MEM con-
taining 1% formaldehyde for 20 min at 37 C followed by the addition of
0.375 m glycine for 5 min to cross-link protein/DNA complexes, har-
vested in PBS, and centrifuged. Cell pellets were resuspended and lysed
in 200 ?l of PBS containing 1% sodium dodecyl sulfate (SDS), 10 mm
Tris-HCl (pH 8.0), 0.5 mm dithiothreitol, protease inhibitors (15 ?g/ml
aprotinin, 1 ?g/ml leupeptin, 2 ?g/ml antipapain, 10 ?g/ml benzami-
dine, 1 ?g/ml chymotrypsin, and 1 ?g/ml pepstatin), and 1 mm phe-
nylmethylsulfonyl fluoride. Cell lysates were sonicated (five cycles, 10
sec/cycle) using a model 450 sonifier (Branson, Danbury, CT) to obtain
DNA fragments of 0.5–1 kb, and debris was removed by low-speed
centrifugation. For each chromatin extract supernatant, one ninth was
kept as DNA input (positive control), and the remainder of each was
diluted 1:10 in dilution buffer containing 0.01% SDS, 16.7 mm Tris-HCl
(pH 8.0), 16.7 mm NaCl, 1.5 mm EDTA, and protease inhibitors. Diluted
samples were separately incubated overnight at 4 C in the presence of
normal rabbit serum (negative control) and antibodies against JunD,
FosB, Fra1, Fra2, CREB-1, CREB-2, C/EBP? proteins (Santa Cruz Bio-
Sepharose beads for 2 h and subsequently washed two times with PBS;
TABLE 1. Detail of primer sets used
Primers Sequences
5?-Flanking isolation
GGTTAATTTTCAACCCTTTCCTGG (forward)
CCCCACGAAGAAGCTCATGAGTG (reverse)
Deletious mutants
GTAGGTACCGGTTAATTTTCAACCCTTTCCTGG (forward)
GTAGGTACCAGAAAGAGATGTCAAAGCTGCC (forward)
GTAGGTACCGTTAGAGTGGGGAAGCTAGCACCG (forward)
GTAGGTACCCACTGGAGATAGTTCAGAATTGGCC (forward)
GTAGGTACCGCCTCAGACAGTGAGTTAATTGTG (forward)
GTAGGTACCAACACATTTAAAGATGTGGAAACCAG (forward)
GTTCTCGAGATCTCAGTCGTAGAGAAGGAATGTCC (reverse)
GAGTTGCCAATGCAA ATGTTTTAGAGGATTTGCCTCA (forward)
TGAGGCAAATCCTCTAAAACATTTGCATTGGCAACTC (reverse)
GCTTGACAATGTTTAGAAATAACTGAAGTATCAG (forward)
CTGATACTTCAGTTATTTCTAAACATTGTCAAGC (reverse)
GG AGGGCGCTTGCTGATTTTACAGCTGAAAAA AAAA (forward)
TTTTTTTTTCAGCTGTAAAATCAG CAAGCGCCCT CC (reverse)
AGACAGTGAGTTAAGGACGTAACTCTACAGCAGAAAATGC (forward)
GCATTTTCTGCTGTAGAGTTACGTCCTTAACTCACTGTCT (reverse)
GAGTTGCCAATGCAAATGAGTCAGAGGATTCGACTCGGACAGTGAGT (forward)
ACTCACTGTCCGAGTCGAATCCTCTGACTCATTTGCATTGGCAACTC (reverse)
CTTTCCAAGTAAGTGAATGCGGAAATGTAGATGATCGTTC (forward)
GAACGATCATCTACATTTCCGCATTCACTTACTTGGAAAG (reverse)
EMSAs (as probes)b
GAGTTGCCAATGCAAATGAGTCAGAGGATTTGCCTCAGACAGT (forward)
ACTGAGGCAAATCCTCTGACTCATTTGCATTGGCA (reverse)
GGAGGGCGCTTGCTGACCTCACAGCTGAAAAAAAAA (forward)
TTTTTTTTTCAGCTGTGAGGTCAGCAAGCG (reverse)
TACAAGCAGCTAGAGGCAGCC (forward)
CTTCAAGGTCATGAC ATTTCCTG (reverse)
GTTTCCAGTAGATTCCACCC (forward)
TCCACCACCCTGTTGCTGTA (reverse)
aItalic letters indicate the mutation sites.
bBold letters are for consensus AP1 and CRE motifs and italic letters for the C/EBPb element.
Sayasith et al. • Regulation of Bovine TNFAIP6Endocrinology, December 2008, 149(12):6213–6225
Page 4
two times with washing buffer containing 0.1% SDS, 1% Triton X-100,
20 mm Tris-HCl (pH 8.0), 500 mm NaCl, and 2 mm EDTA; and two times
with Tris-EDTA buffer containing 10 mm Tris-HCl (pH 8.0), and 1 mm
EDTA by repeated centrifugation/resuspension cycles. Beads were in-
cubated at 65 C for 4 h in 200 ?l of TE buffer in the presence of 1% SDS
to elute DNA complexes, and treated with proteinase K for 2 h at 50 C
to reverse protein/DNA cross-links. All DNA samples were purified
with the QIAquick nucleotide removal kit (QIAGEN), and PCR was
performed using sense (5?-GTT CTA TAT CCT TAG TTT GAG TTG-3?)
and antisense (5?-AT CTC AGT CGT AGA GAA GGA ATG TCC-3?)
primers specific for the bovine TNFAIP6 promoter. The expected PCR
product (262 bp) containing AP1 and CRE cis-elements was analyzed by
2% agarose gel electrophoresis.
Immunoblot analysis
Cell extracts were prepared from granulosa cell cultures as described
previously (22), whereas the medium of cell cultures was purified by
ultrafiltration using iCON concentrator column as directed by manu-
facturer (Pierce, Rockford, IL). Samples (100 ?g/well) were resolved by
one-dimensional SDS-PAGE and electrophoretically transferred to
polyvinyl difluoride membranes (Amersham Pharmacia Biotech). For
TNFAIP6, membranes were incubated with a goat polyclonal antibody
against human TNFAIP6 (1:100 dilution; Santa Cruz Biotechnologies),
and immunoreactive proteins were visualized by incubation with the
horseradish peroxidase-linked donkey antigoat secondary antibody (1:
5000 dilution) and the enhanced chemiluminescence system (ECL Plus)
according to the manufacturer's protocol (Amersham Pharmacia Biotech).
FosB (1:1000), Fra1 (1:1000), Fra2 (1:1000), CREB1 (1:500), CREB2 (1:1000),
C/EBP? (1:500), or GAPDH (1:2000), and the horseradish peroxidase-
linked donkey antirabbit secondary antibody (1:5000 dilution) was used.
Immunohistochemical analysis
Immunohistochemical staining was performed using the Vectastain
ABC kit (Vector Laboratories), as previously described (25). Briefly,
were prepared, and then the paraffin was removed using a series of
alcohol concentrations. Endogenous peroxidase was quenched by in-
cubating the slides in 0.3% hydrogen peroxide in methanol for 30 min.
After rinsing in PBS for 15 min, sections were incubated with diluted
normal goat serum for 20 min at room temperature. The antihuman
TNFAIP6 antibody (1:500 in PBS) or PBS (negative control) was applied,
and sections were incubated overnight at 4 C. After rinsing in PBS for
10 min, a biotinylated goat antigoat antibody (1:222; Vector Laborato-
ries) was applied, and sections were incubated for 45 min at room
temperature. After washing in PBS for 10 min, sections were incubated
with the avidin DH-biotinylated horseradish peroxidase H reagents for
45 min at room temperature, washed for 10 min, and incubated with the
chromogen substrate diaminobenzidine tetrahydrochloride (Sigma, St.
Louis, MO). Sections were counterstained with Gill's hematoxylin stain
and mounted.
Statistical analysis
One-way ANOVA was used to test the effect of different treatments
and constructs on reporter gene activities. When the results indicated
significant differences (P ? 0.05), Dunnett's test was used for multiple
comparisons of means. Statistical analyses were performed using JMP
software (SAS Institute Inc., Cary, NC).
Gonadotropin-dependent induction of TNFAIP6 mRNA in
bovine preovulatory follicles
To study the regulation of TNFAIP6 mRNA in bovine
follicles during the ovulatory process, preovulatory follicles
were isolated between 0 and 24 h after hCG treatment. Total
RNA were extracted from the follicle wall and analyzed by
RT-PCR. Results showed that levels of TNFAIP6 mRNA
were low at 0 h but significantly increased after hCG treat-
ment ?Fig. 1, A and B; n ? 4 follicles, except n ? 2 at 6 h after
hCG (each obtained from different animal)?.
To determine the cellular localization of TNFAIP6 in bo-
vine preovulatory follicles, RT-PCR analyses were per-
formed using RNA preparations of granulosa and theca cells
isolated from preovulatory follicles obtained between 0 and
24 h after hCG treatments. Results indicated that levels of
TNFAIP6 mRNA were very low at 0 h but highly increased
from 6 to 24 h after hCG in granulosa cells (Fig. 1C), whereas,
at 6 and 12 h after hCG, but decreased thereafter (Fig. 1D).
Low levels of TNFAIP6 mRNA in both follicular cells de-
tected before hCG treatment (0 h) (Fig. 1, C and D) are
consistent with that observed with the intact follicle wall at
the same time point (Fig. 1A). In any case, levels of GAPDH
mRNA remained relatively constant at all times of the study.
To verify the induction of TNFAIP6 protein, immunohis-
tochemistry was performed with sections of bovine follicles
isolated before and after hCG. Results indicated that follicles
obtained before hCG treatment (0 h) were characterized by
a compact granulosa cell layer and a very low immuno-
reactivity of TNFAIP6 (Fig. 1E), whereas treatments of
hCG caused a marked increase of staining in the extra-
cellular space (extracellular matrix) of granulosa cell layer
in follicles isolated at 18 (Fig. 1F) and 24 h after hCG (Fig.
1G) during which a loosening (i.e. expansion process) of
the layer was observed. At both time points, immuno-
staining intensity of TNFAIP6 was very low in the theca
interna layer, in keeping with low levels of its mRNA
detected in this cellular population. As expected, no im-
munostaining was observed with sections of follicles 24 h
after hCG treated with PBS (Fig. 1H).
Regulation of TNFAIP6 in primary cultures of bovine
granulosa cells
Because TNFAIP6 expression was regulated in granu-
losa cells in vivo, primary cultures of granulosa cells were
used to study the activity of TNFAIP6 promoter. Cells
were isolated from bovine dominant follicles and cultured
in the absence and presence of FSK for various times. Such
primary cultures have been used to study the regulation
of bovine genes as occurred in vivo (22, 26–28). Results
from RT-PCR analyses showed that levels of TNFAIP6
mRNA were very low or undetectable before FSK treat-
ment (0 h) but significantly increased from 12 to 72 h after
FSK, compared with 0 h (Fig. 2, A and B; n ? 3 independent
experiments). Western blot performed with media of cul-
tures and whole-cell extracts of granulosa cells revealed
that the intensity of the band corresponding to the pre-
dicted size of TNFAIP6 protein (38 kDa) was low in culture
media or undetected in cell extracts before FSK treatment
(Ctl) but highly increased by 24 h of FSK treatment in both
samples (Fig. 2C).
Characterization and transactivation of the bovine
TNFAIP6 promoter
To characterize the promoter activity of bovine TNFAIP6,
a 1767-bp DNA fragment of TNFAIP6 located immediately
Endocrinology, December 2008, 149(12):6213–6225Sayasith et al. • Regulation of Bovine TNFAIP6
Page 5
FIG. 1. Gonadotropin-dependent regulation of TNFAIP6 mRNA in bovine preovulatory follicles. Total RNA of follicle wall (A and B), granulosa
cells (C), and theca interna (D) were prepared from bovine preovulatory follicles isolated at 0, 6, 12, 18, and 24 h (Hrs) after hCG and analyzed
by RT-PCR for TNFAIP6 and GAPDH content. A, Representative results of TNFAIP6 and GAPDH mRNA levels are presented from one follicle
per time point. B, The TNFAIP6 signal was normalized to the corresponding GAPDH level, and results are presented as ratios of TNFAIP6
to GAPDH ?mean ? SEM; n ? 4 distinct follicles (each from a different animal) per time point; except n ? 2 at 6 h after hCG?. Bars marked
with an asterisk are significantly different from 0 h after hCG (P ? 0.05). Numbers on the right (A) indicate the size of the PCR fragment.
Granulosa (C) and theca cells (D) from two distinct follicles per time point are presented. Immunohistochemistry of TNFAIP6 protein was
performed on formalin-fixed sections of preovulatory follicles isolated at 0 (E), 18 (F), and 24 h (G) after hCG treatment. H, Control staining
from the follicular tissue presented in G with the use of PBS. The limits of the granulosa cell (GC) layer are indicated. The region above the
(TI). Magnification, ?400.
Sayasith et al. • Regulation of Bovine TNFAIP6 Endocrinology, December 2008, 149(12):6213–6225


text:
Email AddressVerified
Phone Number(▒▒▒) ▒▒▒- ▒▒ 43
228 Friends
Reviewed774 Reviews
Offline IDPersonal Info
Offline IDPassport
- School
- Pakuranga College, Huntington High School
- Work
- Rick's Cafe Americain, Ministry of Works
- Languages
- English
- Rick And Liz's Airbnb Symbol
Hey, I'm Rick And Liz!
I am originally from NYC. Moved to New Zealand when I was 17 with my mother and father and two sisters. Went to Auckland University and graduated with a degree in Architecture. Started playing the trumpet when I was 8 and worked my way through University playing in bands.
Moved to New Orleans about three years ago with my New Zealand wife, Liz and we love it.
Rick And Liz has completed online and offline ID verification.
Wish Lists (1)
Reviews (771)
Reviews From Guests
Rick and Liz were present to our needs and questions from the moment we arrived to their warm good byes... the home was charming and fun and the accomodation was gorgeous.
Wonderful stay, Rick, Liz and Avery + cats and dog are always here to make you feel confortable. Their house is perfectly located and it's really pleasant to ride bicycle all over the city. Don't hesitate! / Super séjour à NOLA, une famille et ses animaux toujours disponibles pour vous faire sentir comme à la maison. Très bien situé, de toutes façons, il est facile de faire du vélo partout. N'hésitez pas! THANK YOU LIZ, RICK & AVERY! AS+M
Originally, I booked a room with a different layout, but was pleasantly surprised to stay in a cozy little attic room with an ensuite bathroom. The room is air conditioned, and maintained a comfortable temperature throughout our stay. This room is super cozy, and would be great for a couple or single person. I shared the room with my cousin, and we were both very comfortable here. The neighborhood is old, quiet, and charming. A walk to the Quarter was about 15-20 minutes going at a leisurely pace. Safety is always a concern of mine, and I never felt unsafe in this area. My cousin and I did walk home a few nights between midnight at 3 AM, and the streets were well lit. Uber rides were between $6-$8 to/from the Quarter. Rick is very friendly, and willing to share any tips and recommendations within the neighborhood and city. His hospitality was superb, and I felt like I was sharing a home with a longtime friend. I can't wait to come back for a visit!
This was by far one of the best airbnb experiences I've ever had. Liz and Rick were absolutely wonderful and I felt more than welcome in their home. They were perfectly accommodating and there was no want for anything.
Rick et Liz sont des personnes très accueillantes. Ils ont été de très bons conseils pour diverses sorties marquantes de notre voyage hors des endroits touristiques. Ils ont été toujours disponibles durant notre séjour! Le cottage créole est un bel endroit et la chambre était telle que décrite, propre et chaleureuse; les photos la représentent bien. La maison se situe dans Tremé, un quartier tranquille à 15 mn à pied du French Quarter, et 5-10mn en vélo! (Rick et Liz prêtent des vélos à leurs visiteurs!). Le trajet en vélo de la maison vers les quartiers plus touristiques se fait parfaitement bien! Merci Rick et Liz pour ce beau séjour, nous avons maintenant notre adresse à La Nouvelle Orléans!
Rick and Liz were wonderful hosts! They were considerate and never made you feel like you were in their way. They have a warm and peaceful home. The animals are also super friendly (shout out to Sgt pepper). The location is about a 20 min walk from the French quarter. It's about $7 uber ride from the French Quarter and pretty close to the city park area.
I am new to airbnb and this was a great experience. Rick and Liz made me feel very welcome to their home and were very helpful in making recommendations for music venues that i would be interested in. The area is very interesting musically and everybody I met while walking around said hello, and were relaxed and friendly. The room as pictured is delightful, a quite neighbourhood - I slept well.
Enjoyed my stay at the Creole Cottage very much! Great location for exploring. Easy walk to the quarter. Used the bike to explore City Park and further. Rick and Liz were wonderful hosts and very interesting to chat with. Definitely recommend! Make sure you go to the Pagoda at least once.
Rick and Liz were really nice hosts, always here to help us, with the smile!! Just perfect. The place was really clean, with a garden! The bedroom very spacious.. we really enjoyed our stay with them!!
The room is lovely, as are the many household cats. The location is convenient. We had a car, but were able to walk to the most common tourist destinations in half an hour. Rick and Liz also offered bikes to help get around. Take this listing if you like interesting places and hosts.
Reviews From Hosts
Rick and Liz only spent one night with us but they were great guests (and so was Sgt. Pepper). They were fun to chat with and very respectful of our home. We would love to have them back for a longer stay any time.
Rick and Liz were wonderful guests. They stayed for the weekend with their dog and were a pleasure to host. The room was absolutely spotless on checkout.
Rick and Liz and Sgt. Pepper were easy and a treat to host. They are the type of guests you want to host, and wish all the others were more like them.
Rick and Liz are experienced Air B&B hosts, and they were so appreciative of their stay here, which made me very happy! They are truly a wonderful couple. Very at ease, friendly, and just a pleasure to have here. Their dog Sgt. Pepper was my first dog guest, and he was quiet and calm around my cats, and very sweet. I hope to visit Rick and Liz in New Orleans at the home they have so lovingly restored. They are interesting, such fun to talk with, and very respectful and caring. I'd love to host them again.
This was a magical experience with Nina and Dale and their 3 pussycats in Nina's wonderful stone cottage in the gorgeous gorgeous rolling hills of Wisconsin. Nina's surveyor uncle discovered the property in total disrepair in the early 1960's and she grew up with her family restoring it. She has a photo album recording the painstaking step by step process and what a marvellous final result.There are tramping tracks galore and lovely critters everywhere and I could go on and on and on but why don't you go and discover this lovely place and these lovely people for yourselves.
One of the best reasons for belonging to Airbnb is meeting people like Rick and Liz ( and Sargent Pepper- their dog) who were fun to have around. They were relaxed and felt comfortable with us in our home. We shared lots of stories about architecture, FLW and pets. We felt as we made new friends. Their enthusiasm was contagious which makes hosting very easy.
We have had lots of wonderful people stay with us and Rick & Liz were two of our favorites! We now have new friends who are welcome at our place any time and as often as they can come back. We hope we can go see them in New Orleans!! And their sweet, sweet dog Sgt. Pepper was a star with us and our dogs! Thank you Rick and Liz! We loved having you all as our guests and we would love to see you again!
Rick and Liz were wonderful guests! I so enjoyed meeting them! They were communicative and friendly, easy going and fun! I would host them again anytime!
Thank you for staying at my bnb. Please know you are welcome back anytime. Safe and happy travels!!
I couldn't wait to leave my review for these two great people. Maybe it was partly because we are fellow airbnb hosts, but we had an instant rapport. They're extremely friendly people, fun-loving and great conversationalists. I feel like I've made two new friends. I can't wait to come and be a guest in their airbnb in New Orleans. You're lucky if you get to host Rick and Liz!
References (8)
I have known Rick and Liz for about 10 years; met them through my partner Ronnie Taylor when Rick and Ronnie played in the same band. Rick is an amazing horn player and as Ronnie says, one of the top notch trumpet players in the world. Rick is fun to be around, talented, charming, kind and have always found him to be courteous and respectful of other people. Always has a big smile on his face. What to say about Liz, there are probably not enough adjectives to describe her; she is one of life's beautiful people, smart and so funny and when talking to you, makes you feel like you are the most important person in the world. Being around Rick and Liz is like going to watch a beautiful movie and feeling warm fuzzies for days after. I would highly recommend anyone wanting fabulous accommodation in New Orleans to stay at Creole Cottage, not only do you get wonderful accommodation but you have the privilege to meet two most fabulous people who will make your stay a talking point for a long time. Selma Broad. New Zealand
If you want to stay in an great place with interesting, well-travelled people this is the place for you. Rick will steer you towards the best places to listen to jazz and Liz is the shopping queen. You couldn't meet a more vibrant couple anywhere in the world.
As a member of the local Jazz and Blues music club I had frequently listened to Rick Roff's admirable talent as a performer. At the same time his wife Liz made her vivacious personality evident in the audience. It wasn't until I viewed a prospective property for sale that I realised her day job was as an Estate Agent while Rick was an Architect. These two engaging people have combined all their talents in hosting guests at their darling Creole Cottage. Knowing these two live wires I would definitely recommend a stay with them anytime for the simple pleasure of meeting them. Seeing New Orleans is an added bonus!
Rick and Liz are two of the nicest people you will ever meet, and their house is one of the coolest places you will ever find in your travels. You may arrive as a guest, but you will leave as a friend.
This man and wife are amazing people! If you have the good fortune to cross paths with them be sure you'll be blessed. Soft spoken, good hearted Rick Roff is also a sensitive and accomplished jazz musician who can put a gorgeous touch to anything he plays. Lovely Liz is the most lively person on Earth who has the gift of putting into a vibrant prose anything she sees around her. Once we get our travelling shoes on, we'll come down to NOLA for the Rick Roff Trio reunion and enjoy this great place you restored so tastefully. We wish you all the success and continuing happiness. Lots of love, Lorenzo, Nubia and Bernadette (woof-woof).
Rick is an all-round top guy - warm, friendly, kind, a great cook, hot jazz trumpet player, can build, design and gentrify - what more could you want in a man? And he's married to Liz, one helluva sassy woman! They used to be my neighbours in NZ, and I miss them.
To all you travellers out there that are looking for that something special and memorable in your travels, you just have to visit New Orleans one and only "Creole Cottage in Historic Treme with hosts Liz & Ric. I met Liz many years ago in a business capacity when she was selling Real Estate and I was a Mortgage Broker, well still am, and we formed a fantastic everlasting friendship ever since. She sold my house for me, even did my gardening before an open home as it wasn't up to her gorgeous green fingered standards and guess what - it sold! Ric is an unbelievably talented builder, architect, chef, musician - you name it he's done it sort of a guy. They have watched our kids grow up and our girls were their flower girls when they were married. What more can I say than, if you don't go and visit them then you will miss out on two very entertaining characters who have travelled the world and have lots of great stories to share with you. Enjoy your stay Best wishes Robyn Edwards
Rick and Liz are super friendly. My wife Colleen and I stayed with them at the Creole Cottage in February 2012, together with another couple from New Zealand. We had a fantastic stay. Liz told us where the good shopping is. Rick introduced us to the Jazz scene. They explained their restoration work on the cottage. We walk down tree-lined Esplanade to the French Quarter every day and returned every evening to a cheerful welcome and a clean, comfortable room.


text:
Top Definition
Halo Map Tools, also know as HMT, is an unofficial Halo modifying tool, created by MonoxideC, that helps modders create simple to extreme mods.
Bill: Hey, I just created a Halo mod with Halo Map Tools!
Fred: I give a shit?
Fred: I give a shit?
by mastersync23 December 09, 2008


text:
Category Archives: RSF – REPUBLICIAN SINN FEIN
New laws attack right to free speech and political expression
A letter which appeared in yesterdays Irish News Tuesday August 26 2014, from RSF president (Republican Sinn Fein) Des Dalton.
THE announcement by the 26-county justice minister, Frances Fitzgerald, of draconian new laws marks a direct attack on the right to hold or communicate political opions and ideas as set out in the UN (United Nations) Universal Declaration of Human Rights as well as the International Covenant on Civil and Political Rights.
With the centenary of the 1916 Easter Rising fast approaching it is obvious that the 26-county administration is attempting to silence Irish Republicanism and to drive it underground. Such coercive methods have failed in the past as this present effort will also fail. The proposed new laws are intended to silence those who refuse to accept the normalisation of British rule in Ireland and the continued partition of our nation. However, these measures can also be extended to cover all forms of political dissent, be that political, social or economic. People need to be awake to this fact and speak out now. Civil and political rights bodies need to protest against this gratuitous attack on the human rights of Irish citizens. Article 19 of the UN Universal Declaration of Human Rights states: "Everyone has the right to freedom of opinion and expression; this right includes freedom to hold opinion and expression; this right includes freedom to hold opinions without interference and to seek, receive and impart information and ideas through any media and regardless of frontiers." Almost 100 years ago Padraig Mac Piarais (Patrick Perse) warned the predecessors of today's political class: "They think they have pacified Ireland. They think that have purchased half of us and intimidated the other half. They think they have foreseen everything, think that they have provided against everything; but the fools, the fools, the fools – they have left us our Fenian dead, and while Ireland holds these graves, Ireland unfree shall never be at peace." It is evident that the political establishment have not absorbed the lessons of history. They think that they can imprison an idea, that by locking up republicans they can suppress the desire for a free and independent Ireland. Irish republicanism has withstood centuries of repression at the hands of both the 26-county and British states. It has enjured and will continue to do so because it lives in the hearts and minds of the Irish people. Ireland unfree shall never be at peace!!!
With many thanks to: Des Dalton, Uacharan, Republican Sinn Fein (RSF), Dublin 1
COLOUR party uniforms belonging to Republican Sinn Fein (RSF) were seized during police raids in Craigavon last week. http://www.donegaldemocrat.ie/webimage/1.5560656.1381077197!/image/3090683238.jpg_gen/derivatives/articleImgDeriv_628px/3090683238.jpg The uniforms were due to be used during annual Easter commemoration in Lurgan this Saturday. It is understood as many as 18 uniforms were taken from a house in the Drumbeg estate on Friday. Sources say the uniforms were bought to replace two sets previously taken by the RUC/PSNI and they are regularly used during events organised by the hardline party. The same set of uniforms is believed to have been used by a guard of honour at the funeral of Co Donegal republican Joe O'Neill (pictured above) who died last year. The items recovered included trousers, jumpers, boots, scarves and berets. While it is not clear why they were seized it is understood a number of people arrested in the North Armagh area recently have been questioned about images that appeared in a calendar produced by Republican Sinn Fein. RSF spokesman Martin Duffy condemned the RUC/PSNI operation, claiming it was "heavy handed." "This happens the same time every year when Republican Sinn Fein is holding a commemoration," he said. "It's just harassment to try and stop it but they won't stop it." Meanwhile, a shotgun was also discovered during a search of another property nearby on Friday. A 49-year-old man and 52-year-old woman arrested at the time were later released. With many thanks to: Connla Young, The Irish News.
CIRA RSF REPUBLICAN SINN FEIN IRELANDs see retweet Irish Blog http://goo.gl/dUixAO
The Prison authorities' stance on compassionate leave for Republican Prisoners must be challenged.
Statement from the Charlie Agnew Cumann, Republican Sinn Féin, Armagh City
"Republican Prisoners have a long history of honouring their word when on compassionate leave. The current British administration seems intent on punishing Republican prisoners during the most difficult and vulnerable time they can go through" according to a statement from the Charlie Agnew Cumann, Republican Sinn Féin, Armagh City, on June 1, 2012.
"Republican prisoners who are currently incarcerated in Maghaberry prison are enduring the most horrendous conditions and barbarity at the hands of the British administration, propped up by all the politicos in Stormont. The prisoners have to go through invasive strip searching; controlled movement is imposed on Republican landings and free association completely removed.
"Life as a Republican prisoner is one of hardship and daily challenges. Due to the inhumane conditions, the Republican prisoners have been on a no wash protest to try and end the unacceptable conditions. Now it would seem Republican prisoners are facing the refusal of compassionate parole as a means to punish them for their political beliefs and protest.
"In January 2012, protesting POW Damien McKenna was refused compassionate parole to attend his father's funeral with no recourse to appeal the decision. The decision was engineered to be made after 4pm which meant the High court was finished for the day meaning the prison authorities decision was final. This Machiavellian behaviour in itself is unjust and must be challenged.
"In May 2012, Political Hostage Martin Corey applied for compassionate parole only to be passed from pillar to post for two full days before a decision was made. Finally a decision to allow release for one and a half hours was given 15 minutes before the funeral was due to start for his brother who had died.
"His conditions where deplorable. He was only allowed to the funeral Mass, he arrived at 1pm and had to be back in Jail for 3pm, the jail being more than a half and hours drive, in total he was only allowed with his family to grief for a short period.
"The issue of compassionate parole is an emotive one. Republican prisoners have always honoured their word and the British state, at various times, have always denied leave as a form of punishment.
"We the Republican people must now take this issue to our hearts, we must campaign for better compassionate parole conditions for prisoners and for the refusal of compassionate parole to cease.
"Along with this we must continue in our fight to secure Political status for prisoners and demand that Political hostages Martin Corey and Marian Price are released immediately", the statement concluded.
Related articles
- [R . S . F news] Provo MLAs act as jailers (seachranaidhe1.wordpress.com)
- British Security Forces harass Scottish and English solidarity with Irish Republican Prisoners (seachranaidhe1.wordpress.com)
- Statement from the POW Department Republican Sinn Féin 223 Parnell Street Dublin 1 00353-1-8729747 00353-87-9374277 (seachranaidhe1.wordpress.com)
- Statement from the Family & Friends of Republican prisoners, Maghaberry. 19th May 2012 (seachranaidhe1.wordpress.com)
- Free Marian Price " Don't Let Her Root in Prison ! (seachranaidhe1.wordpress.com)
Wolfe Tone Commemoration
Sunday, 10 June 2012
Wolfe ToneCommemorationBodenstownSunday, June 10, 2012Assemble 2.30pmSallins, Co Kildare
March to Wolfe Tone's grave at Bodenstown
organised by Republican Sinn Féin
Related articles
- [R . S . F news] Provo MLAs act as jailers (seachranaidhe1.wordpress.com)
- Statement from the POW Department Republican Sinn Féin 223 Parnell Street Dublin 1 00353-1-8729747 00353-87-9374277 (seachranaidhe1.wordpress.com)
- If you are looking for lemmings buy a Playstation (sluggerotoole.com)
- McGuinness and Sinn Fein finally return to the pluralist language of Tone… (sluggerotoole.com)
- The Rebel Shamrock Festival (seachranaidhe1.wordpress.com)
tiocfaidh Àr Là FREE THEM ALL STOP FUCKINGNG AROUND , FREE ULSTER, CUT THE SLF CONFUSED TRAITORS. PUSSY WILLIS'S NOT TRUE . WRE CLTS , TAKE THM OU.T OUT USED TO SELL EIRIN. ago
(Tiocfaidh Àr Là) ITS HERE,
Antony Mulvihill
Conspiracy theories have never been strangers to Republican circles. The most notable since the 1998 Belfast Agreement, is that so-called 'securocrats' are involved in a conspiracy to discredit the leadership of the Provisional movement and undermine the peace process. (1) Securocrats can be defined as "disgruntled members of the security forces who want to force Sinn Fein out of government." (2) The securocrats allegedly pull the secret strings of Northern Ireland. "These men, skulking in corners of the army, MI5, Special Branch and the Northern Ireland Office, form… a 'shadow government', bent on forcing its own, reactionary agenda on the province. In this view, their driving purpose is the defeat, discrediting and humiliation of Sinn Féin and the IRA – regardless of the policy pursued by Tony Blair and his 'official' government in Downing Street." (3) For example, last December the late Denis Donaldson was exposed as a British agent. He had been one of three officials charged with spying on other political parties at Stormont; the alleged 'Stormontgate' plot discovered in October 2002. The Provisional leadership insisted that this was proof that the spy ring had never existed and that the whole Stormontgate affair had been set up by the securocrats. "It was a British spy ring controlled by securocrats, by people within the establishment who are hostile to the peace process" declared Martin McGuinness. (4) This is questionable. Far from launching the Stormontgate affair to give the then Ulster Unionist leader David Trimble an excuse to walk away from power-sharing with the Provisional movement, the securocrats took the view that Mr Trimble should ignore the spying scandal and stay in government.
As Ed Moloney puts it, "The Sinn Féin conspiracy theory – that the spooks are out to destroy the peace process – suffers from a fundamental flaw. Not only is it rubbish, but the exact opposite is the truth. The peace process represents the wildest fantasies of the security establishment come true and the last thing the spooks want is to see it destroyed. The peace process has enabled MI5 and the Police Service of Northern Ireland special branch to achieve something that very few if any security forces have ever accomplished: to see their enemy defanged by its own leadership and led out of violent revolutionary ways into constitutional politics and a world where the principle of consent overrides the Armalite. MI5 and the PSNI know they could never have done this themselves, that they needed people like Gerry Adams and Martin McGuinness to do it for them. So why on earth would the spooks want to undermine them, to frustrate them and place obstacles in their way, as the Provo leadership claim they have consistently done – most recently with Stormontgate? To have done so would have been to act fundamentally against their own interests. It just wouldn't make sense." (5) Professor Paul Bew noted that "those whom Sinn Féin named as securocrats gave every sign of being inconvenienced by the Stormontgate affair. It was their job, after all, to deliver the institutions of the Good Friday agreement and to keep Mr Adams locked into the peace process. In that sense, there has been, for many years now, a profound commonality of interest between the British security establishment and Mr Adams." (6)
This is not to say that the securocrats are nothing but "a conspiracy theory that belonged in an airport thriller rather than the real world" as some think. (7) Such elements of the British security forces exist, "but they are not in the driving seat of British security policy. Those running the show know that there is more than one way to skin a cat. They are the type of people whom the Provisional leadership was meeting behind the backs of its membership and whose overriding objective was to ensure that Provisional objectives were never secured." (8) For example, when Chief Constable Hugh Orde alleged in December 2004 that the Provisional movement carried out the Northern Bank robbery, Martin McGuinness told a press conference that there was a political agenda by the government and "securocrats" to try to "criminalise and marginalise" his party and stop the peace process. (9) But it is not the agenda he would have us believe. "It is an agenda of bringing Sinn Fein more closely into the structure of the state. Had there have been any chance of a deal being stuck, Orde would have been under tremendous pressure to go no further than his under reported statement that a republican group was responsible for the Northern Bank heist, without specifying which." (10) Similarly, the Provisional movement accused senior NIO official permanent secretary Pilling of running a nest of securocrats. Pilling had in fact ensured during the 1998 Belfast Agreement negotiations that prisoners would not be released in exchange for decommissioning as a means to facilitate the peace process and the Provisional's transition into British institutions. Far from the British establishment "considering the process to be a threat to its rule in the North" or the securocrats "attempting to engineer political isolation and demonisation" of the Provisional leadership (11), we find British diplomat David Goodall claiming that everything was going almost according to plan and former MI6 director Michael Oatley expressing public admiration for the Provisional leadership. (12)
The Provisional movement also frequently allege that there is a conspiracy between the media and the securocrats, with journalists becoming a willing participant in the so-called 'dirty war' by spreading "misinformation" to attack Sinn Fein and the Peace Process. When the so-called 'Stake Knife' affair broke out in May 2003, the Provisional leadership denied everything and blamed the collusion of securocrats and 'journocrats'. "All of these stories are coming from nameless and faceless securocrats in British intelligence" said Martin McGuinness. (13) The peace process has indeed corrupted journalism, but not in the manner alleged by McGuinness. Quite the opposite in fact. The media has been accused by award-winning journalist Ed Moloney of covering up truth to protect the peace process and being reluctant to report events unhelpful to the peace process. (14) For example, when in October 2000 the Provisional shot dead Joe O Connor in Ballymurphy, their involvement in the murder was mostly brushed under the carpet by the media. Reporters and editors sympathetic to Sinn Fein's strategy branded journalists who asked awkward questions (such as Ed Moloney or Suzanne Breen) "JAPPS – Journalists Against the Peace Process". It would be more accurate to say that the peace process has in fact produced Journalists Against Journalism.
The securocrat conspiracy theory may be flawed, but it brings two political benefits for the Provisional movement. First it allows to present itself nationally and internationally as a perpetual victim; which is electorally advantageous. Secondly, it allows to shift the blame for deadlocks in the peace process away from itself to 'securocrats', 'malevolent blanketeers', 'japps' and other 'enemies of the peace process'. But the cost has been that crying securocrat wolf too often has ultimately undermined the credibility of the Provisional movement. Every time the Provisional leadership cries out 'securocrat', only the faithful believe.
NOTES(1) Roy Greenslade, The securocrats' revenge, The Guardian, 9 October 2002. One of the only mainstream articles taking the 'securocrat' conspiracy theory seriously. (2) Nicholas Watt and Rosie Cowan, Special branch blamed for leaks that damage Sinn Fein, The Guardian 23 April 2002(3) Jonathan Freedland, The strange collusion between Downing Street and Sinn Fein, The Guardian, 21 December 2005 (4) Henry McDonald, 20 Years of treachery, The Observer, 18 December 2006(5) Ed Moloney, Was there a Stormontgate? The Belfast Telegraph, 21 December 2005(6) Paul Bew, Shadowy alliance haunts Stormontgate, The Yorkshire Post, 22 December 2005(7) Jonathan Freedland, op.cit.(8) Anthony McIntyre, SF – Securocrat Fantasists,/(9) Mathew Tempest, IRA blamed for £22m Belfast bank raid, The Guardian 7 January 2005(10) Anthony McIntyre, op.cit.(11) Adam O'Toole, 'Stakeknife' turns out to have blunt British blade, An Phoblacht/Republican News, 22 May 2003(12) David Goodall, Actually it's all working out almost exactly to plan, Parliamentary Brief, May/June 1998, and Michael Oatley, Forget the weapons and learn to trust Sinn Fein, Sunday Times, 31 October 1999(13) Tom Happold and agencies, Adams: Scappaticci innocent until proven guilty, The Guardian, 16 May 2003(14) Henry McDonald, Reporters 'covered up truth' about IRA to help peace, The Obs
Related articles
- Now is the Time to Take a Stand Against the Anti Good Friday Agreement Unionists (seachranaidhe1.wordpress.com)
- Sinn Fein's Martin McGuinness offers to meet dissidents (belfasttelegraph.co.uk)
- Jeffrey Donaldson: IRA must say sorry (belfasttelegraph.co.uk)
- McGuinness in attack on dissidents (belfasttelegraph.co.uk)
- Martin McGuinness accuses dissident republicans of being enemies of Ireland (guardian.co.uk)
[R . S . F news] Provo MLAs act as jailers
IN A statement on May 25 the POW Department of Republican Sinn Féin said that the use of compassionate leave as a punishment against POLITICAL HOSTAGE Martin Corey, who is currently languishing in Maghaberry Jail with no charges against him, ws a new low even for the British and their lackeys in Stormont.
"Peter Corey, Martin's brother, died on Monday, May 21, 2012 after a battle with cancer. The prison regime refused Martin compassionate leave. One of the reasons given was their fear that Martin would abscond. Republicans have always honoured their word in regards to compassionate leave and to suggest otherwise is insulting. What is equally insulting was the suggestion by the prison service was that 'the lives of those attending the funeral would be endangered' should he get out for the day.
"An appeal was scheduled for the morning of the funeral – Thursday, May 24 at 9am. The High Court in Belfast refused to make a judgement; referred it back to prison service and suggested they reconsider their refusal.
"At 11.45am, 15 minutes before the funeral Mass was scheduled to begin, Martin's solicitor was informed that leave had been granted on the condition that two British Ministers belonging to the Provisionals escort him. One other independent person was also to accompany Martin. Martin was not allowed out of the Chapel grounds, even for the burial and had to be back in Maghaberry for 3pm.
"The escort by Provisional Crown Ministers is a classic case of poacher turned gamekeeper. The sight of O'Dowd and McCartney – ex-Republicans – performing the duties of the prison service shows them to be hollow shells of their former selves, something which was not lost on the community they claim to represent.
"The Provisionals are so totally embedded in the British establishment that they were entrusted with the 'job' of ensuring a Republican POW returned to jail after attending a Mass. Had Martin Corey been given dignified parole he would have returned to the jail at his appointed time, a man of his word does not need jailers.
"If the Provisionals have the power to act as jailers in the name of the British government and the prison service, how come they don't use that power to get Martin released? Or even get the inhuman conditions in the jail improved?
"The practices of the past are no longer afforded to Republican POWs, most recently POW Damien McKenna was point blank refused leave to attend his father's funeral.
"The cat-and-mouse games of the NIO and courts in relation to compassionate leave for Republican prisoners is mental torture against people who are already going through deep grief and anguish, this practice must stop immediately."
POSTED ON BEHALF OF : by Republican Sinn Féin on Friday, 25 May 2012 at 15:10
Related articles
- PoW Martin Corey refused compassionate leave on the death of his brother (seachranaidhe1.wordpress.com)
- Statement from the POW Department Republican Sinn Féin 223 Parnell Street Dublin 1 00353-1-8729747 00353-87-9374277 (seachranaidhe1.wordpress.com)
- Report on MagHaberry for UNHRW (seachranaidhe1.wordpress.com)
- Statement from the Family & Friends of Republican prisoners, Maghaberry. 19th May 2012 (seachranaidhe1.wordpress.com)
- Free Gerry McGaough campain letter (seachranaidhe1.wordpress.com)


text:
Aptamers have until recently been tightly held by IP restrictions, Base Pair Biotechnologies Founder Bill Jackson presents information and data they have generated on aptmers in diverse assays such as biosensor and lateral flow. Presentation is from the American Society for Cell Biology meeting Dec 16, 2012
What's it really like to be an engineer or a scientist? What do they really do all day? You're about to find out! Meet the next generation of engineers and scientists in these profiles of young professionals, who may just inspire you to join them. Beth Mooradian is fascinated by how the body works, always ready with more questions. Her job allows her to travel the world finding the answers. Find out what it's like to be an exercise scientist.
Presentation by Dr. Damasa B. Magcale-Macandog, Professor, UPLB CAS, University of the Philippines Los Banos, Philippines. Delivered during the International Conference on Climate Change Impacts and Adaptation for Food and Environmental Security, November 21-22, 2012 at SEARCA, UPLB, College, Laguna, Philippines.


text:
What are Singles Events?
Singles events are nights of entertainment and social connection, as well as opportunities for you to invite your single friends. They are typically social in nature, allowing you to meet other singles and make friends. They're also a great way to bring together singles from all six of our churches.
These Singles events typically happen three times a year: in spring, summer and fall. They include our do.justice service event as well as our annual Christmas Event.
Our next Singles event is:
Fall Singles Series
The Singles Series is an environment for all singles that takes place three times a year. Each series has three meetings, held on Tuesday evenings at 7:30 p.m. in the Auditorium at Buckhead Church. Single adults gather for worship and teaching on a topic that is relevant to them. Singles Series are designed to gather singles from all six of our churches and provide an environment for corporate worship. These events also help singles get connected into Singles Gatherings.
When: Tuesday Sept 15, Sept 22 and Sept 29
Who: Single Adults
What Time: Service starts at 7:30pm, but come early at 7pm and join us in the lobby for free coffee, dessert, corn hole and other fun games… it's a great way to meet new people before the night gets started!
Where: Buckhead Church Auditorium
Cost: It's FREE and no registration is required.
This event is for single adults only. Childcare will not be provided.


text:
Available in Aqua, Black, Coral
Style Deals- A chiffon dress featuring a high-low hem and a racerback. Partial button placket. Shirred elastic waist. Partially lined. Woven. Lightweight.DETAILS: 33-52" approx. length from high point shoulder to hem, 32" chest, 24" waist Measured from Small 100% polyesterHand wash cold, dry flat Imported


text:
317 Views 1 Reply Latest reply: Mar 5, 2013 3:37 PM by John Galt
John Galt Mar 5, 2013 3:37 PM
Re: Buying a new charger is the only way when it broken without any man-made reason after warranty? in response to zhaoxue217
Troubleshooting MagSafe adapters
Using and maintaining your Apple MagSafe Adapter
Reducing cable strain on your MagSafe power adapter
Whether or not to replace the power adapter under warranty is left to Apple's judgement. If you believe it malfunctioned due to a manufacturing defect, you must prove your claim.


text:
Super 8 Lisbon
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
TripAdvisor Reviews Rocks Lane B&B Balcombe
Travel Blogs from Lisbon
Out of Sturgis
... along the fringe of Orange. That color orange... bad on a map. Well I wasn't on the fringe. No!! I was in it. 50 mph wind, sideways rain and intermittent HAIL. I ended up following a mobile home because I could see it's tail lights. About the only thing I could see.
This is, as my good friend Firm Lock would say, a white knuckle ride. Leaning into the wind, following tail lights, and watching my navigation to see when I could ...
The Land of 10,000 Lakes
Today we had an interesting day, crossing Wisconsin and Minnesota, and landing in Fargo, ND. The day began at 7:55 and 70 degrees with overcast skies. And it wasn't long before the rains came. The skies were threatening and it was clear we had a front passing through. At one point, in addition to the rain, we had a heavy black header with a white stripe below it, a patch of blue peeking through and crooked fingers of gray rising from below like many smoke ...
Day 4: Aberdeen, South Dakota
... almost an hour of lightning on the horizon...though the storm barely brushed past our little campsite. Tomorrow, we will set off diagonally across The state heading to Murdo to see the Pioneer Auto Museum before continuing west to Badlands NP, Mt. Rushmore, Sturgis, Deadwood, and into ...
On the move to Montana
... We ate indoors.
On our way to Stop 3 we passed thru the tiny town of Circle. It was about the only sign of life in the thousands of acres of farmland (growing wheat) that we came to.
The views across the massive rural landscape left us in awe even though we have seen this sort of limitless view across the plains before. It does make the sky look larger and you seem to be able to see the curve of the earth on the horizon.
One night only in Minnesota
This entry is only to keep my map current. We came across the eastern part of North Dakota, past Fargo and into Minnesota where we stayed at a great city park for the night. All grass and, with ************e there, peaceful.
I went for a run in the morning and then we headed across Minnesota towards Duluth...past ALL those lakes in Minnesota! So many of them.


text:
Town Square
Cutting off comments
Original post made by Walter_E_Wallis, Midtown, on Oct 28, 2007
Comments (1)
a resident of another community
on Oct 31, 2007 at 4:04 pm
Thanks for this posting.
I agree and I find it disturbing to see articles, like the insulting one on Rumsfeld posted on Town Square, locked so that no comments can be posted. What does this communicate? It is one thing to screen out inappropriate comments, but quite another to stop all comments. If comments are not allowed then the story should not be on Town Square.
Don't miss out on the discussion!
Sign up to be notified of new comments on this topic.
Su Hong Menlo Park changes hands after 38 years
By Elena Kadvany | 25 comments | 8,758 views
"Woman on Fire" a new Book for Sexual Empowerment
By Chandrama Anderson | 0 comments | 1,045 views
Guy Fawkes: Historical vs Misappropriated
By Douglas Moran | 4 comments | 852 views
The Food Party! Top Ten List
By Laura Stec | 0 comments | 806 views
Fee Waivers
By John Raftrey and Lori McCormick | 1 comment | 707 views


text:
A Project of the Annenberg Public Policy Center
Click here to read more
Q: Are 65,000 Syrian refugees being relocated to the U.S.?
A: No. The Obama administration says 1,000 to 2,000 Syrians will likely be resettled in the United States by the end of September, and at least 10,000 more in 2016.


text:
editor's review
VirtualWiFi provides you with a very interesting application software to help you quickly and easily enable the wireless network adapter on your computer connect to several wireless networks. While this may sound impossible with just one card, there are ways to achieve this through hardware virtualization.
There are a number of scenarios where it is desirable to have a wireless contrivance connect to multiple networks simultaneously. Currently, this is possible only by utilizing multiple wireless network cards in the contrivance. Haplessly, utilizing multiple wireless cards causes extortionate energy drain and consequent reduction of lifetime in battery operated contrivances.
VirtualWiFi facilitates simultaneous connections to multiple networks by virtualizing a single wireless card. The wireless card is virtualized by introducing an intermediate layer below IP, which perpetually switches the card across multiple networks. The goal of the switching algorithm is to be transparent to the utilizer who optically discerns her machine as being connected to multiple networks.
The design, implementation, and performance of the VirtualWiFi system, alongside the analyzed and evaluated buffering and switching algorithms in terms of delay and energy consumption, make the application agnostic of the upper layer protocols, and works well over popular IEEE 802.11 wireless LAN cards.
It should be possible for a utilizer to transmute individual parameters of each virtual adapter, and these always active adapters should withal be able to send and receive packets at any time. VirtualWiFi achieves this by multiplexing the wireless card across multiple networks.
Overall, the VirtualWiFi computer program utilizes an adaptive network hopping scheme where a card gets a time slot, called the Activity Period, for each network operating on a particular channel. The sum of the activity periods over all the connected networks is called the Switching Cycle.
top FREE alternatives
top PAID alternatives


text: This hotel leans on its historic background. The room was lovely very colonial in style but the service was not up to the standard I expected. Breakfast you could be served quickly or you were left waiting. They did not seem to have enough people to cope with the number of people in the restaurant. The same could be said... More


text:
Genetically Modified Foods Confuse Consumers
Genetically modified foods have been around for years, but most people in the United States have no idea if they are eating them.
The Food and Drug Administration says such foods don't need to be labeled, so some states are moving forward on their own.
Vermont recently became state the first to require labeling of genetically modified organisms (GMOs). Bills and ballot initiatives are pending in many more.
What about the rest of the country? And does labeling matter?
There's much confusion about genetically modified foods and their safety.
Opponents, who at times have protested in the streets, say consumers have the right to know whether their food contains GMOs. The food industry and companies that genetically engineer seeds have pushed back against the labeling laws, saying GMOs are safe and labels would be misleading.
A look at the debate and some of the facts about genetically modified foods:
GMOs are hard for the average consumer to grasp. You can't touch or feel a GMO.
Genetically modified foods are plants or animals that have had genes copied from other plants or animals inserted into their DNA.
It's not a new idea.
Humans have been tinkering with genes for centuries through selective breeding. Think dogs bred to be more docile pets, cattle bred to be beefier or tomatoes bred to be sweeter. Turkeys were bred to have bigger breasts -- better for Thanksgiving dinner.
What's different about genetically modified or engineered foods is that the manipulation is done in a lab. Engineers don't need to wait for nature to produce a desired gene. They speed up the process by transferring a gene from one plant or animal to another.
What are the desired traits? Most of the nation's corn and soybeans are genetically engineered to resist pests and herbicides. A papaya in Hawaii is modified to resist a virus. The FDA is considering an application from a Massachusetts company to approve a genetically engineered salmon that would grow faster than traditional salmon.
Most of the genetically modified corn and soybeans are used in cattle feed or are made into ingredients such as corn oil, corn starch, high fructose corn syrup or soybean oil.
Even in some of those products, the manufacturing process eventually may remove some of the modified genes.
A few fruits and vegetables are engineered -- the Hawaiian papaya and some squash and zucchini, for example. Only a small amount of sweet corn, the corn we eat, is genetically modified.
But there's no genetically modified meat or fish, like the fast-growing salmon, currently in the market for human consumption. The FDA has yet to approve any.
The vast majority of scientific research has found genetically engineered foods to be generally safe.


text:
- Load more related videosLikeAboutShareDownloadAdd to27,286 views92% 66 5From:Pornstars: + SuggestSuggest new pornstarsxSuggestedSubmitAdded on: 2 years agoShow moreShare with FriendsEmbedStart at:
Please Sign In560 x 340
Add to playlistJump to your favorite actionSequence
- 560 x 340
- 640 x 360
- 853 x 480
- 1200 x 720
All Comments (4)
Login or Sign Up now to post a comment!1 year agoHubby and i bang a cute college twink like that occasionally. Dude LOVES the dick. Actually wants to have another dude or two to join us to bang him.. that we havn't set up yet..1 month ago2 months agoTreasure Island still doesn't "get" they're decades overdue for either disappearing, or doing something beyond sad meth losers in the same old scenes.
But now you see how meth turns you into an embarrassment1 year ago[[dateAdd]][[commentMessage]]0
Playlists Containing This Video
Flag This Video
155 videos Play All View PlaylistTimTales and Lucio Saints Hot 682 favorites93%
47 videos Play All View Playlist
102 videos Play All View Playlisttimtales 68 favorites95%
The World's Biggest XXX Porno Tube
The PornHub team is always updating and adding more porn videos every day. It's all here and 100% free porn. We have a huge free DVD selection that you can download or stream. PornHub is the most complete and revolutionary porn tube site. We offer streaming porn videos, downloadable DVDs, photo albums, and the number 1 free sex community on the net. We're always working towards adding more features that will keep your porno addiction alive and well. Send us feedback if you have any questions/comments.
- Information
- Terms & Conditions
- Privacy Policy
- 2257
- Work With Us
- Content Partners
- Advertise
- Webmasters
- Amateur Payment Program
- Press
- Support and Help
- Contact Support
- Feedback Forum
- Sitemap
- Discover
- Pornhub Blog
- Insights Blog
- Mobile / Tablet
- More


text:
Mount Stewart
This section of the page features an image gallery, so if you're using a screen reader you may wish to jump to the main content.
Notice: Please note that the Coach House is closed until further notice, we apologise for any inconvenience caused.
Neo-classical house and celebrated gardens
Mount Stewart is one of the most inspiring and unusual gardens in the National Trust's ownership.
The garden reflects a rich tapestry of design and great planting artistry that was the hallmark of Edith, Lady Londonderry. The mild climate of Strangford Lough allows astonishing levels of planting experimentation. The formal areas exude a strong Mediterranean feel and resemble an Italian villa landscape; the wooded areas support a range of plants from all corners of the world, ensuring something to see whatever the season.
Mount Stewart House
The house has now re-opened after our 3 year long restoration project.We've brought back the elegance and charm of the house when it was home of the 7th Marchioness Edith, Lady Londonderry and her family in the early 20th century and we look forward to welcoming you to enjoy in all its splendour.
To find out more about the house and what we've done, visit our house page.
Festival of Light returns
Building on the success of the past two years, Mount Stewart will once again host a magical light show – but this year with a winter theme.
With the support of Tourism NI, Festival of Light will open from Friday 20 November until Sunday 29 November. Based on the Son et Lumiere shows that originated in France this year's show promises to be a fascinating display.
So much to see
There are so many things to see and do at Mount Stewart this autumn, you'll be hard pressed to fit it all into one visit.
Take a look at our ideas to help inspire your visit to Mount Stewart.
Whether you're bringing your family, friends, or just visiting by yourself, you'll find plenty of things to spark your interest.
The family residence has been recently restored to its former glory in a bid to preserve the building and its vast collection for future generations to enjoy.
Discover the breathtaking Central Hall and the more intimate rooms as previously lived in by the family.


text:
Sports Photos
A look at the five best signees in Oklahoma's 2014 class
Photo: @AllAmericaGame/Twitter
Justice Hansen, QB -- Hansen is the lone signal caller in this offense-laden class, especially at the top. Considered one of the top quarterbacks in the class, he might be the only one the Sooners need.
Related Content
To post a comment, log into your chosen social network and then add your comment below. Your comments are subject to our Terms of Service and the privacy policy and terms of service of your social network. If you do not want to comment with a social network, please consider writing a letter to the editor.