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Heteromultimers of DEG?ENaC subunits form
H?-gated channels in mouse sensory neurons
Christopher J. Benson*†, Jinghui Xie*, John A. Wemmie‡, Margaret P. Price*, Jillian M. Henss*, Michael J. Welsh§,
and Peter M. Snyder*
Departments of *Internal Medicine and‡Psychiatry, and§Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242
Contributed by Michael J. Welsh, December 17, 2001
Acidic extracellular solution activates transient H?-gated currents
in dorsal root ganglion (DRG) neurons. The biophysical properties
of three degenerin?epithelial sodium (DEG?ENaC) channel sub-
units (BNC1, ASIC, and DRASIC), and their expression in DRG,
suggest that they might underlie these H?-gated currents and
function as sensory transducers. However, it is uncertain which of
these DEG?ENaC subunits generate the currents, and whether they
to large mouse DRG neurons differed from BNC1, ASIC, or DRASIC
expressed individually, but were reproduced by coexpression of
the subunits together. To test the contribution of each subunit, we
studied DRG from three strains of mice, each bearing a targeted
disruption of BNC1, ASIC, or DRASIC. Deletion of any one subunit
did not abolish H?-gated currents, but altered currents in a manner
consistent with heteromultimerization of the two remaining sub-
units. These data indicate that combinations of two or more
DEG?ENaC subunits coassemble as heteromultimers to generate
transient H?-gated currents in mouse DRG neurons.
These currents were thought to mediate acid-induced pain
sensation (2). However, their molecular basis remained uncer-
tain until the recent discovery of degenerin?epithelial sodium
channels (DEG?ENaC), which reconstitute several properties of
these currents: H?activation, Na?selectivity, and amiloride
Within the DEG?ENaC family, three genes encode H?-gated
channels: BNC1 (3), ASIC (4), and DRASIC (5).¶BNC1 and
ASIC each have alternative splice forms [BNC1a and BNC1b (3,
6) and ASIC? and ASIC? (7), respectively], which differ in a
segment including the N terminus and first membrane-spanning
domain. mRNA corresponding to each of these subunits are
present in mammalian dorsal root ganglion (DRG; refs. 7–10),
and protein expressions for some of the subunits have been
identified at sensory nerve terminals (9–11) where they may
participate in the transduction of acid-mediated pain (8, 11) and
mechanosensation (9, 11).
Expression of BNC1, ASIC, or DRASIC individually in
heterologous cells generates transient H?-gated Na?currents.
Moreover, when coexpressed in combination, they associate (12,
13) and produce currents with unique functional properties (6,
12, 13), suggesting that they have the capacity to form hetero-
multimers. Previous data comparing the properties of native
ENaC subunits provide some insight into the in vivo channel
composition. For example, the properties of DRASIC match the
H?-gated current properties in rat cardiac sensory neurons (14).
subunits is lacking, and the precise composition of the channels
in vivo remains uncertain.
Our goal, therefore, was to determine the functional compo-
sition of the DEG?ENaC channels in DRG neurons. We used
two strategies. First, we compared the properties of the currents
in mouse DRG neurons with those of the cloned mouse channel
ransient proton-activated currents in peripheral sensory
neurons were first described more than 20 years ago (1).
subunits expressed individually or in combination. Second, we
examined the properties of the currents in genetically altered
mice lacking BNC1, ASIC, or DRASIC.
Materials and Methods
Culture of DRG Neurons. Thoracic DRG neurons were collected
and dissociated from 6- to 12-month-old mice as described (9).
DRGs were dissociated with papain, collagenase, and dispase,
plated on poly(D-lysine)?laminin-coated plastic, and stored at
either room temperature in L15 medium supplemented with 50
nM nerve growth factor, or at 37°C in F12 medium supple-
mented with nerve growth factor. Neurons 20–35 ?m in diam-
eter were studied 18–48 h after plating.
cDNA Constructs. Mouse BNC1a was cloned from mouse brain
mRNA (CLONTECH) by reverse transcription–PCR by using
the 5? primer: 5?-CCA TCG ATG GAG CCA TGG ACC TCA
AGG AGA GCC CCA GC-3?, and 3? primer: 5?-G GGG TAC
Mouse ASIC? and ASIC? were cloned by reverse transcription–
PCR from total RNA isolated from mouse brain and DRG,
respectively, by using the primer pairs: ASIC? (5? primer:
G-3?; 3? primer: 5?-CGG GGT ACC TTA GCA GGT AAA
GTC CTC AAA CG-3?), ASIC? (5? primer: 5?-GCC ATC GAT
ATG GAG CTG GAT GAG GGT GAC TC-3?; 3? primer:
CG-3?). Mouse DRASIC was cloned as described (15). The
constructs were cloned into pMT3 for expression in COS-7 cells.
Heterologous Expression of cDNA in COS-7 Cells. cDNAs were
expressed by electroporation (950 ?F, 320 V, GenePulser II;
pH-evoked current. cDNA for green fluorescent protein (3
?g/0.4 ml) was expressed with DEG?ENaC subunits (15 ?g/0.4
ml) to facilitate detection of transfected cells by epifluorescence.
For DEG?ENaC coexpression experiments, cDNAs were trans-
fected at equal concentrations for a total of 15 ?g/0.4 ml. Cells
were cultured in DMEM with 10% FBS and 1% Pen?Strep at
37°C and were studied 48–72 h after transfection.
Electrophysiology. Whole-cell patch-clamp recordings (at ?70
mV) from DRG neurons and COS-7 cells were performed with
an Axopatch 200B amplifier (Axon Instruments, Foster City,
CA) and acquired and analyzed with PULSE?PULSEFIT 8.30
Abbreviations: DRG, dorsal root ganglion; DEG?ENaC, degenerins?epithelial sodium
†To whom reprint requests should be addressed. E-mail: firstname.lastname@example.com.
¶BNC1a has also been named MDEG (33), BNaC1 (34), and ASIC2a (8), and its splice variant
BNC1b has been called MDEG2 (6) and ASIC2b (8). ASIC? has also been named BNaC2 (34)
called ASIC3 (8).
The publication costs of this article were defrayed in part by page charge payment. This
article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C.
§1734 solely to indicate this fact.
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February 19, 2002 ?
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no. 4 www.pnas.org?cgi?doi?10.1073?pnas.032678399
Page 2
(HEKA Electronics, Lambrecht, Germany) and IGOR PRO 3.16
(WaveMetrics, Lake Oswego, OR) software. Currents were
filtered at 5 kHz and sampled at 2 or 0.2 kHz. Micropipettes (2–5
M?) were filled with internal solution: 100 mM KCl, 10 mM
EGTA, 40 mM Hepes, and 5 mM MgCl2, pH 7.4 with KOH.
External solution contained: 120 mM NaCl, 5 mM KCl, 1 mM
MgCl2, 2 mM CaCl2, 10 mM Hepes, 10 mM Mes, pH adjusted
with tetramethylammonium hydroxide, and osmolarity adjusted
with tetramethylammonium chloride. Extracellular solutions
were changed within 20 msec by using a computer-driven
solenoid valve system (16).
We focused on the transient H?-activated currents because
they are believed to be carried solely by DEG?ENaC channels
(8), whereas the sustained transient H?-activated currents are
probably the result of multiple channel types, including the
vanilloid receptors (17, 18). Data are means ?SEM unless
otherwise stated. Kinetics of desensitization and recovery from
desensitization curves were fit with single exponential equations
and time constants (?) reported. pH activation curves were fit by
using the Hill equation: Fraction of open channels ? 1/{1 ?
(pH10/K0.510)n}, where K0.5is the pH at which half of the channels
are opened.
Generation of ASIC? and BNC1 Knockout Mice. The generation and
description of the BNC- and DRASIC-null mice have been
reported (9, 11). Mice with disruption of the gene for ASIC
(J.A.W. and M.J.W., unpublished work) were generated by
similar methods. All mice were F2 generation from 129?
Sv:C57BL-6 crosses. Wild-type littermates from BNC1 and
ASIC knockout mice were analyzed separately, however, no
differences were observed between the two groups and so their
data were pooled.
Individual DEG?ENaC Subunits Do Not Reproduce the H?-Gated Cur-
rents of DRG Neurons. To determine which subunits generate
transient acid-evoked currents in DRG neurons, we compared
currents in mouse DRG with those of mouse H?-gated DEG?
(BNC1b does not form homomeric H?-gated channels; ref. 6).
We focused on three properties that distinguish between indi-
vidual subunits (14). First, the DEG?ENaC subunits form
channels with differing sensitivities to pH. We found that the pH
sensitivity of DRG neurons did not match that of DRASIC,
ASIC?, or BNC1a (Fig. 1B). Although DRG neurons and
ASIC? showed similar half-maximal activation, the slopes of
their pH-activation curves differed markedly. Thus, a single
H?-gated DEG?ENaC subunit cannot reconstitute the pH
sensitivity of DRG neurons.
Second, we measured recovery from desensitization. After
prolonged exposure to acidic pH, DRG currents recovered by
?50% in 300 ms (Fig. 1C). Expression of DRASIC or BNC1a
generated currents with similar recovery times, but ASIC? and
ASIC? currents recovered more slowly. These data indicate that
neither ASIC? nor ASIC? alone account for H?-gated currents
in DRG neurons. However, the rate of recovery from desensi-
tization does not exclude channels composed of DRASIC or
BNC1a subunits.
Third, we examined desensitization kinetics during an acid
stimulus. DRASIC desensitized faster than BNC1a, ASIC?, or
ASIC? (Fig. 1 D and E). Currents from mouse DRG neurons
desensitized even faster than any of the individual subunits.
These data (summarized in Table 1) indicate that no single
DEG?ENaC subunit generates the acid-evoked currents ob-
served in DRG neurons; none of the individual subunits
reproduced the pH sensitivity, recovery from desensitization,
and desensitization kinetics, suggesting the currents result
from coexpression of two or more DEG?ENaC subunits in the
same cell. Subunits could assemble as homomultimers, with
total native current equaling the sum of current from the
various homomultimers. However, this arrangement could not
explain our finding that acid-evoked DRG current desensitized
faster than any individual subunit. Alternatively, subunits
could assemble into heteromultimers containing two or more
of the DEG?ENaC subunits. Such a model could explain the
data if coassembly generated a channel with faster kinetics
Coexpression of BNC1a, ASIC?, and DRASIC Mimics the pH-Evoked
Current in DRG Neurons. To test the hypothesis that multiple
DEG?ENaC subunits coassemble to generate DRG H?-gated
Fig. 1.
by any one DEG?ENaC subunit. (A) Representative acid-evoked currents in a
wild-type DRG neuron and COS-7 cells expressing the indicated DEG?ENaC
subunits. The bar above each current represents a fast solution change from
pH 7.4 to pH 6, except for BNC1a, which is evoked by pH 5 (because of the
relative pH insensitivity of BNC1a channels). Vertical scale bar: 2.71 nA DRG,
1.23 nA DRASIC, 1.78 nA ASIC?, 0.943 nA ASIC?, 3 nA BNC1a. (B) Effect of pH
on currents from DRG and COS cells expressing the indicated subunits from a
holding pH of 8. Currents were normalized to those evoked by pH 5 (except
BNC1a, which was normalized to pH 4 current). Lines are fit of Hill equation.
n ? at least four cells for all data points. (C) Time course of recovery from
desensitization. Current was completely desensitized by a prolonged pulse to
pH 6 (pH 4 for BNC1a). Cells then were bathed in pH 7.4 solution for the
Recovery is percentage of current evoked by the second pulse compared with
the first. Lines are fit of single exponentials. n ? at least three cells for each
data point. (D) Superimposed currents evoked by a solution change from pH
7.4 to pH 6 (pH 4 for BNC1a). Vertical scale bar: 1.27 nA DRG, 0.36 nA DRASIC,
1 nA ASIC?, 4.95 nA BNC1a. (E) Mean time constants of desensitization as
measured from single exponential fits to the falling phase of the currents
evoked by pH 6 (pH 4 for BNC1a) application to wild-type DRG neurons (n ?
29) and COS-7 cells expressing indicated subunits (n ? at least five cells).
Properties of acid-evoked currents in DRG neurons are not matched
Benson et al.
February 19, 2002 ?
vol. 99 ?
no. 4 ?
Page 3
currents, we coexpressed BNC1a, ASIC?, and DRASIC. Coex-
pression reproduced the fast DRG desensitization kinetics;
current desensitized faster than any individual subunit (Fig. 2 A
and B). Coexpression also reproduced the DRG pH sensitivity
(Fig. 2C) and recovery from desensitization (Fig. 2D) reasonably
well. Thus, BNC1, ASIC, and DRASIC subunits assemble in
some combination as heteromultimeric channels.
pH-Evoked Current in DRG Neurons from BNC1-Null Mice. To test
directly which subunits contribute to DRG proton-gated cur-
rents, we studied neurons from mice with targeted deletions in
BNC1, ASIC, or DRASIC. Deletion of BNC1 did not abolish
acid-evoked currents (Fig. 3A) or alter the peak amplitude
(Table 1), but dramatically altered current properties. Cur-
rents in BNC1-null mice were more pH sensitive (Fig. 3B)
(pH0.5? 6.6, BNC null; 6.2, wild type), and recovered more
slowly from desensitization (Fig. 3C) (? ? 0.68 sec, BNC null;
0.42 sec, wild type), than neurons from wild-type mice. Thus,
BNC1 contributes to H?-gated currents in wild-type DRG
neurons. However, deletion of BNC1 did not slow current
desensitization (? ? 0.15 ? 0.01 sec; Fig. 3A), and because it
was faster than any individual subunit, this finding suggests
that more than one DEG?ENaC subunit coassembles to
generate currents in BNC1-null neurons.
We therefore tested the hypothesis that two remaining sub-
units, ASIC and DRASIC, generate the acid-evoked currents in
the BNC1-null mice. Coexpression of ASIC? and DRASIC
generated acid-evoked currents that desensitized faster than
currents from either subunit expressed alone, reproducing the
fast kinetics of currents of BNC1-null mice (Fig. 3D). Coexpres-
sion also reproduced the pH sensitivity and recovery kinetics of
the BNC1-null mice (Fig. 3 E and F). These data suggest that
ASIC and DRASIC form heteromultimeric channels in BNC1-
null neurons.
pH-Evoked Current in DRG Neurons from Neurons ASIC-Null Mice. To
test whether ASIC contributes to DRG acid-evoked currents, we
studied neurons from mice containing a targeted deletion of
ASIC. Similar to BNC1-null neurons, deletion of ASIC did not
eliminate acid-evoked current (Fig. 4A), but altered its proper-
nA; wild type, 4.07 ? 0.45 nA, P ? 0.01). Second, currents in the
ASIC-null neurons desensitized more slowly than wild-type
Table 1. Functional properties of mouse DRG neurons and DEG?ENaC subunits expressed
individually and in combination (in COS-7 cells)
(nA at pH 5)
maximum pH
? desensitization
? recovery
(sec, at pH 7.4)
DRG neurons
ASIC null
BNC1 null
DRASIC null
DEG?ENaC subunits expressed
Coexpressed DEG?ENaC subunits
DRASIC ? BNC1a
ASIC? ? BNC1a
DRASIC ? ASIC? ? BNC1a
4.1 ? 0.45
1.4 ? 0.18*
3.9 ? 1.00
0.15 ? 0.01
0.20 ? 0.02
0.15 ? 0.01
2.66 ? 0.40*
0.32 ? 0.02*
4.0 ? 0.35*
1.3 ? 0.20*
2.8 ? 0.55*
0.14 ? 0.01
0.26 ? 0.02*
0.90 ? 0.20*
0.18 ? 0.02
Values without standard errors are derived from curve fits of the means (n ? 3–14 for each data point on the
curves). Current amplitude at pH 5 (n ? 22–65); ? desensitization at pH 6, except BNC1a is at pH 4 (n ? 5–29).
Asterisks denote statistical differences from wild-type DRG for data with standard errors of the mean (P ? 0.01,
two-tailed t test).
Fig. 2.
the pH-evoked current in wild-type DRG neurons. (A) Currents evoked by
application of pH 6 from pH 7.4 solutions in a DRG neuron, and a COS-7 cell
coexpressing DRASIC, ASIC?, and BNC1a. Vertical scale bar: 1.32 nA DRG, 0.5
nA COS-7. (B) Mean time constant of desensitization of the current evoked by
pH 6 application to COS-7 cells expressing all three subunits (n ? 6; other bars
represent data collected in Fig. 1 for comparison). (C) pH dose-response data
six cells for all data points) compared with wild-type DRG neurons. All data
were collected and fit as per Fig. 1.
Coexpression of DRASIC, ASIC?, and BNC1a mimic the properties of
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neurons (ASIC null, 0.20 ? 0.02 sec; wild type, 0.15 ? 0.01 sec,
P ? 0.014), although still faster than individual DEG?ENaC
subunits (Fig. 4A). Third, the ASIC-null cells recovered faster
from desensitization (ASIC null, ? ? 0.20 sec; wild type, ? ? 0.42
sec) (Fig. 4C). These altered properties indicate that ASIC
contributes to the acid-evoked current in wild-type DRG neu-
rons. In contrast to BNC1-null neurons, deletion of ASIC did not
alter pH sensitivity (pH0.5? 6.2) (Fig. 4B).
Two findings in ASIC-null neurons could not be explained
by the summation of currents from individual DEG?ENaC
subunits; the currents desensitized (Fig. 4D) and recovered
from desensitization (Fig. 4F) faster than either DRASIC or
BNC1a currents. Coexpression of DRASIC and BNC1a re-
produced both properties (Fig. 4 D and F). Moreover, the pH
sensitivity of ASIC-null neurons was reproduced by coexpres-
sion of BNC1a and DRASIC, but not by either subunit alone
(Fig. 4E). These data suggest that DRASIC and BNC1 coas-
semble as heteromultimers to generate H?-gated channels in
ASIC-null mice.
pH-Evoked Current in DRG from DRASIC-Null Mice. To test the
contribution of DRASIC, we studied DRG currents in mice with
a targeted deletion in this subunit. We focused on the rate of
desensitization, because this rate was a key property in differ-
entiating heteromultimeric channels from individual DEG?
ENaC subunits.?H?-gated currents in DRASIC-null mice de-
sensitized almost 10-fold slower than neurons from wild-type,
and BNC1 reproduced these slow kinetic values. Thus, DRASIC
unpublished data.
neurons in a manner consistent with expression of the remaining subunits.
(A) Representative currents evoked by changing the solution from pH 7.4
to pH 6 in wild-type and BNC1-null DRG neurons demonstrating similar
desensitization rates. Twenty-six percent of BNC1-null DRG (n ? 82) re-
59% of wild type (n ? 112). Vertical scale bar: 1 nA wild type, 0.38 nA BNC1
null. (B) pH dose-response and (C) recovery from desensitization data for
BNC1-null DRG neurons (n ? at least eight cells for all data points) com-
pared with wild type. (D) Superimposed currents evoked by a solution
change from pH 7.4 to pH 6 in a BNC1-null DRG neuron, and COS-7 cells
expressing indicated subunits demonstrating that only coexpression of
subunits reproduced the fast kinetics of the BNC1-null neuron. Vertical
DRASIC ? ASIC?. (E) pH dose-response data and (F) recovery from desen-
six cells for all data points). Also shown are the BNC1-null DRG data from
B and C and the fits of the data for subunits expressed individually for
comparison. Data were collected and fit as per Fig. 1.
Targeted disruption of BNC1 gene alters pH-evoked current in DRG
neurons in a manner predicted by expression of the remaining subunits. (A)
Representative currents evoked by a solution change from pH 7.4 to pH 6 in
wild-type and ASIC-null DRG neurons demonstrating slightly slower desensi-
tization of the ASIC-null neurons. Forty-eight percent of ASIC-null DRG (n ?
48) responded to pH 5 with greater than 150 pA transient current. Vertical
scale bar: 1 nA wild type, 0.47 nA ASIC null. (B) pH dose-response and (C)
recovery from desensitization data for ASIC-null DRG neurons (n ? at least 12
cells for all data points) compared with wild type. (D) Currents evoked by
application of pH 6 from pH 7.4 in an ASIC-null DRG neuron and COS-7 cells
expressing indicated subunits demonstrates that only coexpression of sub-
nA ASIC-null neuron, 0.4 nA DRASIC, 5.5 nA BNC1a, 0.41 nA DRASIC ? BNC1a.
(E) pH dose-response data and (F) recovery from desensitization data for
DRASIC and BNC1a coexpressed in COS-7 cells (n ? at least seven cells for all
data points). Also shown for comparison are the ASIC-null DRG data from B
and C and the fits of the data for subunits expressed individually. Data were
collected and fit as per Fig. 1.
Benson et al.
February 19, 2002 ?
vol. 99 ?
no. 4 ?
Page 5
also contributes to the formation of H?-gated channels in
wild-type DRG neurons. Moreover, DRASIC is a necessary
component of the native channel; the fast desensitization kinetic
(Fig. 5B).
Our data indicate that three DEG?ENaC subunits, BNC1,
in mouse DRG neurons. Coexpression of all three subunits
reproduced the biophysical properties of the DRG currents.
Moreover, targeted disruption of BNC1, ASIC, or DRASIC did
not abolish the acid-evoked currents, but altered the properties
in a manner predicted by residual expression of the other two
Do DEG?ENaC Channels Assemble as Homomultimers or Heteromul-
timers in DRG Neurons? There are two general ways the DEG?
ENaC subunits might generate H?-gated channels. BNC1,
ASIC, and DRASIC might form homomultimeric channels.
Conversely, two or more subunits might assemble to form
heteromultimeric channels.
In distinguishing between these two alternatives, the rate of
current desensitization was the most informative property. Acid-
evoked currents in DRG neurons desensitized faster than any
individual DEG?ENaC homomultimeric channel. This property
is not explained by the sum of multiple homomultimeric chan-
nels; the desensitization rate in this case would be intermediate
between the rates for the individual subunits. On the other hand,
coexpression of two or more DEG?ENaC subunits reproduced
the fast desensitization kinetics. Desensitization was also fast in
mice lacking either BNC1 or ASIC, suggesting the remaining
subunits formed heteromultimers in these mice.
The rate of current recovery from desensitization was also
informative in differentiating homo- from heteromultimers.
Currents in ASIC-null mice recovered from desensitization
faster than any individual DEG?ENaC homomultimeric chan-
nel. Coexpression of DRASIC and BNC1a reproduced this
property, suggesting that they form heteromultimeric channels.
Our data, showing that DRASIC (11) and BNC1 (9) are
expressed in most medium to large DRG neurons, support these
Heteromultimerization seems to be a common arrangement
for the DEG?ENaC family of channels: the mammalian epithe-
lial Na?channel (ENaC) is formed by three subunits (?, ?, and
?) (19, 20), and the degenerins in Caenorhabditis elegans are
composed of multiple related subunits (21).
What Is the Subunit Composition of the Heteromultimeric DEG?ENaC
Channels in DRG Neurons? Coexpression of DRASIC, ASIC?, and
BNC1a reproduced relatively well the properties of the transient
acid-evoked currents in mouse DRG neurons. Thus, it is possible
that these three subunits form a heteromultimeric channel.
However, other subunit combinations could also form H?-gated
channels in the DRG.
First, channels might be composed of two subunits. For
example, the cell might express a mixture of BNC1?DRASIC
because coexpression of these combinations produced currents
with properties similar to coexpression of the three subunits.
Moreover, our knockout animals do not allow us to differentiate
the relative contribution of the BNC1 and ASIC splice variants.
Nevertheless, our data suggest that DRASIC is necessary to
reproduce the fast desensitization kinetics of DRG neurons;
targeted disruption of DRASIC markedly slowed desensitiza-
tion. However, DRASIC alone is not sufficient to explain fast
desensitization; currents produced by DRASIC expressed alone
desensitized more slowly than those from DRG neurons,
whereas coexpression of DRASIC with either ASIC or BNC1
reproduced the fast desensitization of native currents.
Second, additional DEG?ENaC subunits might contribute to
the channel complex. For example, ENaC subunits are located
in sensory nerve endings (22, 23), and transcripts of BLINaC
(24) and SPASIC (25, 26) have been detected in the nervous
system. Although protons do not gate these subunits by them-
selves, they could coassemble with BNC1, ASIC, and DRASIC
to form H?-gated channels. Other as-yet-unidentified DEG?
ENaC family members might also contribute to the channel
complex. Although we cannot exclude these possibilities, cur-
rents in BNC1, ASIC, and DRASIC knockout mice had bio-
physical properties that matched remarkably well with coexpres-
sion of the other two known H?-gated subunits. Thus, BNC1,
ASIC, and DRASIC apparently are the predominant DEG?
ENaC subunits responsible for transient H?-gated currents in
DRG neurons.
Although most transient H?-gated currents from DRG have
properties similar to those we observed, previous studies de-
scribed small subsets of cells with different properties. For
example, a minority of rat DRG cells have markedly slower
kinetics of desensitization (2, 16, 27). Although in this study of
mouse neurons we did not observe such currents, they are likely
generated by ASIC subunits; the slow kinetic values are consis-
tent with ASIC? and ASIC?, and these currents are blocked by
psalmotoxin 1, a tarantula toxin specific for ASIC? homomul-
Fig. 5.
pH-evoked current in DRG neurons. (A) Superimposed currents evoked by a
DRG, 2.2 nA DRASIC-null DRG, 1.39 nA COS-7 cell. (B) Mean time constants of
desensitization of the acid-evoked currents from wild-type and knockout DRG
at least five cells), and coexpressed in COS-7 cells (n ? at least six cells) demon-
strates that only DRASIC coexpressed with other subunits reproduced the fast
desensitization kinetics of the DRG neurons. Currents were evoked by pH 6,
except BNC1a currents, which were evoked by pH 4.
Deletion of the DRASIC gene slows the desensitization kinetics of
2342 ?
www.pnas.org?cgi?doi?10.1073?pnas.032678399 Benson et al.


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(#Fannerisms also marks the brand's launch on Instagram, driving about 2,200 followers in the first week.)
Bob Ruhland, vice president of North American marketing at Buffalo Wild Wings, says the brand has used Instagram Direct because it wanted to be "as cutting-edge as possible" and wanted to go "from zero to 60" in a short amount of time.
With Instagram alone, Buffalo Wild Wings fans uploaded nearly 1 million photos to the platform prior to the brand's launch on the photo sharing service. That figure is derived from user-uploaded photos with hashtags like #BuffaloWildWings, which had more than 435,000 mentions, and #BDubs, which had more than 248,000, notes Evan Carpenter, senior community strategist at creative agency Periscope.
While Buffalo Wild Wings is certainly attempting to make the most out of Instagram's Direct feature, few other brands have utilized the product. In fact, since its launch there appears to be only a small handful who have used Direct for marketing purposes. These include Gap, where followers were asked to submit photos in its "What I Wore Today" contest; Kardashian Kollection, which offered a behind-the-scenes photo to followers who reposted an image with #KKDirect, driving 4,600 submissions; Michael Kors, which asked fans to post images with #MKDirect for a special message, generating 900 posts; and GoEnnounce, which asked high-schoolers to send images of their college acceptance letters with the hashtag #GoEccepted in exchange for a welcome to college gift, driving 179 posts.
According to Ian Chee, chief strategy officer at creative and technology agency MRY, Instagram Direct is a one-to-one mechanism that brands tend to use for stunts that will then create buzz. He calls this direct messaging another form of CRM and says brands that use it lose some reach, but can potentially drive buzz and organic reach if consumers actually talk about it.
He says Buffalo Wild Wings' #Fannerisms campaign didn't seem to warrant one-to-one messaging, but notes there is still value in being a first mover.
"I think when new technology comes along, brands jump on the bandwagon without understanding their strategic imperatives. It's to get PR value - 'I can't believe they jumped on so early. They're really forward-thinking,' and that in and itself has its own intrinsic value," Chee says. "I'm not really sure how many people they are going to reach...but trial and error is powerful in this day and age and I commend them for trying, but I'm not sure what success they are going to achieve and I'm not sure what their goals are."
One-on-one communication is also more expensive, but it makes fans feel special because a brand is engaging with them in a more personal way. In the end, Chee says it depends on a brand's priorities and objectives as to whether it is worth sacrificing cost-effectiveness in a gamble to create potentially more powerful and engaging campaigns.
"[Instagram Direct is] not a platform that I delve heavily in with clients," Chee says. "I haven't found a specific reason to recommend it other than being a first mover and getting organic reach out of it. But, as with all new things, they always find new ways to use it."
In addition to photo contests, former media strategist Tessa Wegert says additional branded applications for Instagram Direct include soliciting consumer feedback on brand assets like a new logo or product packaging, as well as conducting surveys or gleaning customer insight on market trends.
In addition to ClickZ and Search Engine Watch, Lisa's work has appeared in The Huffington Post, The Luxury Spot, LearnVest, MarthaStewart.com, GoodHousekeeping.com, amNewYork, and The Wall Street Journal. She's a graduate of Columbia's School of Journalism.
October 21-22, 2015
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October 13, 2015
1pm ET/ 10am PT
November 12, 2015


text:
|Publication number
|Publication type
|Application number
|Publication date
|Dec 27, 1994
|Filing date
|Oct 18, 1993
|Priority date
|Oct 16, 1992
|Fee status
|Also published as
|Publication number
|Ezio Savazzi
|Original Assignee
|Fidia S.P.A
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (4), Referenced by (19), Classifications (10), Legal Events (4)
|External Links: USPTO, USPTO Assignment, Espacenet
The present invention relates to a keyboard for the remote control of machine tools and the like, comprising a plurality of keys and/or controls connected to one or more boards which can generate coded electrical signals indicative of the commands put into effect by the operation of the keys and/or the controls.
In conventional keyboards, all the keys or controls are disposed on a single surface and consequently the item is not easy to grip, particularly if there is a large number of keys or controls.
The object of the present invention is to provide a keyboard which has a large number of keys or controls but which is compact and easy to grip.
According to the invention, this object is achieved by virtue of the fact that the keys and/or controls are divided into at least two sets disposed on at least two faces of a body which can rotate within a tubular casing having a display window, the faces being positionable in the display window in turn as a result of the rotation of the rotary body.
Further characteristics and advantages of the present invention will become clear in the course of the detailed description which follows, purely by way of non-limiting example, with reference to the appended drawings, in which:
FIG. 1 is a perspective view showing the keyboard according to the invention, supported by a pedestal,
FIG. 2 is a perspective view of the keyboard of FIG. 1,
FIG. 3 is a section taken on the line III--III of FIG. 1,
FIGS. 4 and 5 are partially exploded perspective views of the portions indicated by the arrows IV and V in FIG. 2,
FIG. 6 is a section taken on the line VI--VI of FIG. 2, and
FIGS. 7, 8 and 9 are schematic perspective views showing the portion indicated by the arrow VII in FIG. 5, in three different positions.
With reference to FIG. 1, a keyboard, generally indicated 1, for the remote control of a machine tool, generally indicated 2, is connected to the machine tool 2 by means of a cable 4. As can be seen in greater detail in FIG. 4, the keyboard 1 comprises a tubular casing constituted by two semicircular shells 6, 8 which enclose a rotary body 10.
With reference to FIG. 3, the rotary body 10 comprises two longitudinal elements 12 with arcuate cross-sections and two flat faces 14, 16, on which two sets of keys 18, 20 are disposed. A series of boards 22--of known type--for generating coded electrical signals indicative of the commands put into effect by the operation of the keys 18, 20 is disposed within the rotary body 10 in the space defined between the faces 14, 16, and the arcuate elements 12.
As well as the two sets of keys 18, 20, the rotary body 10 carries a pair of wheel-like control members 24 (see FIGS. 3 and 4) the diameters of which are greater than the distance between the two parallel faces 14,16, so that a portion of each member projects from each of the faces 14, 16. The wheel-like control members 24 are connected to respective potentiometers which, typically, are used to bring about a percentage reduction in the speed of rotation of a mandrel and in the speed of advance of a shaft of the tool, after these parameters have been set at a predetermined value by means of the keyboard 1. The faces 14, 16 of the rotary body 10 may also have display devices or the like.
With reference to FIG. 4, the shell 8 has a display window 26 in which the faces 14, 16 are disposed in turn by virtue of the rotation of the rotary body 10 through 180� about its longitudinal axis 28. The rotary body 10 is supported for rotation in the shells 6,8 by means of a pair of bearings 30 which engage tubular extensions 32 (only one of which is visible in FIG. 4) of the body 10. The outer ring of each bearing 30 is housed between two semicircular seats 31 formed at one end of the shells 6, 8. The right-hand end of the tubular casing constituted by the shells 6, 8 in FIG. 4 carries a micrometric adjustment wheel 34 (of known type) for advancing a shaft of the tool 2 micrometrically. Still with reference to FIG. 4, two elements 36 of transparent material with C-shaped profiles are disposed at the ends of the window 26 and lamps 38 situated in correspondence therewith, illuminate the face of the rotary body 10 which is in the display window 26.
With reference to FIGS. 3 and 4, an eyelid-like closure element, indicated 40, is rotatable in the tubular casing 6, 8 in the space between the inner wall of the casing and the outer profile of the body 10. The closure element 40 is made of transparent plastics material and can adopt an open position--shown by a continuous line in FIG. 3--and a closed position shown by a chain line in the same drawing. In the closed position, the element 40 closes the display window 26 and protects the body 10 from external agents (mechanical and liquid), affording maximum security against the accidental operation of the keys 18, 20 or the members 24 when the machine is not manned, whilst enabling the functions to be seen from a distance.
A support element in which the keyboard 1 can be snap-engaged is indicated 42 in FIG. 2. The support 42 is fixed in an adjustable manner to a rod 44 which can be fixed to a pedestal, as shown in FIG. 1, or directly to the structure of the machine 2. Two strips 46 of resilient material applied to the internal surface of the support element 42 have the function of facilitating the snap-engagement of the support 42. The strips 46 also constitute an electrical insulation between the pedestal or the body of the machine tool and the casing of the keyboard. A further function of the strips 46 is to damp vibrations so that the keyboard 1 can also be fixed to parts of the machine 2 which are subject to a great deal of vibration.
With reference to FIGS. 2 and 3, the support element 42 has a pair of protective flanges 48. The flanges act as screens against the ambient and background light to improve the contrast (and hence the readability) of the illuminated face. The flanges 48 also have an anti-collision function to protect the keyboard from accidental impacts with parts of the machine or with stray objects and to prevent the risk of the keys being pressed unintentionally. For this purpose, the flanges 48 are disposed in a manner such that, the line 50 (FIG. 3) joining the ends of the flanges 48 in a cross-section in a plane perpendicular to the axis of the keyboard is situated outside the profile of the keyboard 1.
The keyboard 1 may have a resilient handle (not shown) which enables the keyboard also to be held in one hand when the keyboard is removed from the support 42. The keyboard 1 may also have a sensor which detects whether the keyboard 1 is fitted correctly in the support 42 or whether it is held in an operator's hand. This sensor may activate a signalling system or may prevent some functions of the machine if one of these conditions is not verified.
The mechanism which controls the rotation of the rotary body 10 in the casing 6, 8 will now be described with reference to FIGS. 5 to 9.
With reference first to FIGS. 5 and 6, a pin 52 is fixed to the tubular extension 32 of the body 10, parallel to and spaced from the axis of rotation 28 of the body 10. A first end of a helical tension spring 54 is fixed to the pin 52 and its second end is anchored to a flange 56 fixed to a disc 58 which is fixed to the casing 6, 8 and is slightly spaced from the end wall thereof (see FIG. 6 in particular). A stop element 60, fixed to the flange 56, has a stop surface 62 for the abutment of the pin 52.
The disc 58 carries four idle rollers 64 which support for rotation a cup-shaped element 66 connected axially to the casing 6, 8 by means of radial screws 68 (FIG. 6) inserted in the space defined between the end wall of the casing 6, 8 and the disc 58. The cup-shaped element 66 carries an entrainment member 70 constituted by a disc from which a sector extending through an angle slightly larger than 90� has been removed. The surfaces 72 and 74 of the entrainment member 70 are intended to cooperate with the end 76 of the pin 52.
An operating knob 78 fixed to the cup-shaped element 66 can be gripped by hand in order to rotate the element 66 relative the casing 6, 8. The cup-shaped element 66 also carries an emergency switch 80 with an external push-button 82 which can de-activate all the functions of the machine tool.
The operation of the mechanism which rotates the body 10 will be described below with reference to FIGS. 7, 8 and 9.
In the configuration shown in FIG. 7, the spring 54 restrains the pin 52 against the surface 62 of the stop element 60. In this configuration, the face 14 of the rotary body 10 is in the display window 26. The rotary body 10 is in a position of stable equilibrium since the biasing effect of the spring 54 prevents any undesired rotation of the body 10. In order to rotate the body 10, the knob 78 is operated in the sense indicated by the arrow 84 in FIG. 8. The surface 72 of the entrainment member 70 contacts the end 76 of the pin 52 and rotates the body 10 (see FIG. 8). During this rotation, the spring 54 extends until the body 10 has rotated through 90� about its axis 28. After this, the cup-shaped element 66 and the entrainment member 70 remain stationary and the spring 54 brings the pin 52 into abutment with the surface 62 again, causing the body 10 to perform a further 90� rotation (FIG. 9). In this second configuration, the face 16 of the body 10 is in the display window 26. Starting from the configuration of FIG. 9, the knob 78 is operated in the sense indicated by the arrow 86 in order to return the keyboard to the initial configuration shown in FIG. 7.
Clearly, the mechanism described above enables the rotary body 10 to be rotated through 180� by means of a 90� rotation of the cup-shaped element 66 which can easily be brought about solely by means of a wrist movement.
|Cited Patent
|Filing date
|Publication date
|Aug 8, 1990
|Sep 17, 1991
|Emerson Electric Co.
|Housing assembly for electrical components with window for viewing electrical components, sealing rings, and sleeves accepting different sizes of conduit
|Feb 19, 1991
|Apr 7, 1992
|At&T Bell Laboratories
|Dual position computer arrangement
|Mar 13, 1991
|Jan 19, 1993
|Kabushiki Kaisha Toshiba
|Book type wireless remote control apparatus
|Apr 2, 1993
|Dec 7, 1993
|Kabushiki Kaisha Toshiba
|Portable computer with keyboard and having display with coordinate input tablet rotatably mounted to face either toward or away from keyboard when closed over keyboard
|Citing Patent
|Filing date
|Publication date
|Jun 7, 1995
|Mar 25, 1997
|Ascom Hasler Mailing Systems, Inc.
|Electronic postage scale system and method
|Jan 11, 1996
|Jul 15, 1997
|Deckel Maho Gmbh
|Control console for a program-controlled machine tool
|Jun 7, 1995
|Oct 7, 1997
|Ascom Hasler Mailing Systems, Inc.
|Electronic postage scale system and method
|Jun 7, 1995
|Jul 14, 1998
|Ascom Hasler Mailing Systems, Inc.
|Electronic postage scale system and method
|Oct 14, 1993
|May 18, 1999
|Ascom Hasler Mailing Systems, Inc.
|Electronic postage scale system and method
|Jul 17, 1997
|Aug 17, 1999
|Badewitz; Charles J
|Handy keystroke rotary guide for computer programs
|Jul 16, 1997
|Jan 11, 2000
|Ascom Hasler Mailing Systems, Inc.
|Adjustable display for use with an electronic postage scale system
|Jun 7, 1995
|Nov 28, 2000
|Ascom Hasler Mailing Systems, Inc.
|System and method for an electronic postage scale with variable function keys and window screens
|Jun 7, 1995
|Oct 8, 2002
|Hasler, Inc.
|Electronic postage scale system and method
|Mar 20, 2001
|Jan 27, 2004
|Logitech Europe, S.A.
|Computer keyboard
|Oct 8, 1998
|Jun 22, 2004
|Gateway, Inc.
|Remote control panel
|Aug 3, 2000
|Feb 8, 2005
|Matsushita Electric Industrial Co., Ltd.
|Multi-sided remote control device
|Mar 31, 2007
|Sep 14, 2010
|Ohaus Corporation
|Weight indicator housing with a top and bottom cover attachable together in more than one configuration
|Aug 10, 2012
|Jan 13, 2015
|Originatic Llc
|Mountable device having a pivotable input device support
|Aug 31, 2004
|Mar 2, 2006
|Arthur Rishar
|Remote control storage assembly
|Oct 25, 2012
|Smith Renato L
|Mountable device
|Dec 6, 2012
|Smith Renato L
|Accessory enabling chassis conversion for increased utility of electronic devices
|Mar 28, 2000
|Dec 6, 2001
|Sommer Gmbh Mechanische Fertig
|Handheld transmitter e.g. remote controller has pair of oblong outer housings, one of which is longitudinally slidable with respect to other housing
|Mar 28, 2000
|Dec 4, 2008
|Sommer Antriebs- Und Funktechnik Gmbh
|U.S. Classification
|International Classification
|Cooperative Classification
|European Classification
|Oct 18, 1993
Owner name: FIDIA S.P.A., ITALY
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:SAVAZZI, EZIO;REEL/FRAME:006725/0917
Effective date: 19930914
|Aug 12, 1998
|Maintenance fee reminder mailed
|Dec 27, 1998
|Lapse for failure to pay maintenance fees
|Mar 9, 1999
|Expired due to failure to pay maintenance fee
Effective date: 19981227


text: PokePower:Project Redlink Revision as of 17:21, July 8, 2012 by Latiasfan001 (Talk | contribs) (diff) ← Older revision | Latest revision (diff) | Newer revision → (diff) 10,922pages on this wiki Edit History Talk0 Active This project is part of the PokePower group. Any questions about this Project should be asked on the associated talk page or on the Project leader's talk page. Shortcut:PP:PRLPP:PRPP:Redlink Project Redlink (Codename:Twizzlers) is a project by PokePower. The goal of this project is to create and expand much needed articles. Any user is welcome to join! Just add your signature to the "members" list at the bottom of the page. When you have created a page, use the <s>text</s> tag to show this. Contents[show] Aims Our aim is to create needed articles that are filling up the Wanted pages page. Our other focuses are to add more info to existing articles, add some new info to orphaned pages and correct dead-end pages. References Categories Category:Article stubs Special pages Special:WantedPages Special:ShortPages Special:OrphanedPages Special:DeadendPages Sub-Projects Hoenn Routes Members please focus on creating these pages! Hoenn Route 126 Hoenn Route 132 Hoenn Route 115 Hoenn Route 130 Hoenn Route 129 Created by Latiasfan001 Hoenn Route 109 Hoenn Route 128 Created by Latiasfan001 Hoenn Route 127 Hoenn Route 107 Hoenn Route 125 Hoenn Route 122 Hoenn Route 133 Hoenn Route 108 Cheers LF 001 17:21, July 8, 2012 (UTC) Create 25 random redlinked pages EM013: Skirting Around Surskit I (107 links), EM001: Never Spritz a Knotty Sudowoodo (107 links), FRLG013: Some Things Are Better Left Unown (105 links), EM033: The Final Battle IX (105 links), FRLG016: It Takes Patience, Knowledge and a Really Quick Beedrill (104 links), FRLG034: Storming the Forretress (104 links), FRLG015: Going Green (104 links), FRLG023: A Well-Journeyed Jumpluff (104 links), EM020: Susceptible to Sceptile (104 links), FRLG022: Surprised by Sneasel (104 links), EM005: Gotcha Where I Wantcha, Glalie (104 links), EM004: Pinsir Me, I Must Be Dreaming (104 links), EM011: You Need to Chill Out, Regice (104 links), EM009: A Dust-Up With Dusclops (103 links), FRLG017: Red, Green, Blue and Mewtwo Too (103 links), FRLG025: Bested by Banette (103 links), EM008: Just My Luck... Shuckle (103 links), EM022: A Cheeky Charizard Change-Up II (103 links), EM007: Moving Past Milotic (103 links), EM006: As Luck Would Have It, Kirlia (103 links), FRLG031: Start the Countdown, Starmie (103 links), EM019: Cunning Kirlia (103 links), FRLG020: Secrets from Sneasel (103 links), EM003: Interesting Interactions Involving Illumise (103 links) and FRLG028: Double Down Deoxys (103 links) Expand See Special:ShortPages Contributors The Latias Fan Crimsnnavy C S Retrieved from "http://pokemon.wikia.com/wiki/Pokepower:Project_Redlink?oldid=333350"


text:
Carter's Erin Girls Toddler Mary Jane Flat
Compare at $34.00
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Item # 291036
These fun and fashionable mary jane flat offer a sporty spin on a classic casual look. She will love to show off her Carter's Erin flats!
- Striped textile upper
- Elastic strap for secure fit
- Bow embellishment and patent cap toe
- Textile lining with cushioned insole
- Synthetic sole
- Imported
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By: Wearewinter
Location: Manitoba
February 23, 2014
Cute but elastic digs in
"Too bad these didn't come with a thin Velcro snap. The elastic dug in and left a mark on her feet and she could only wear for an hour before telling me it hurts."
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text:
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- To view this video download Flash Player
Fulfillment by Amazon (FBA) is a service we offer sellers that lets them store their products in Amazon's fulfillment centers, and we directly pack, ship, and provide customer service for these products. Something we hope you'll especially enjoy: FBA items qualify for FREE Shipping and .
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A soccer player is suddenly ripped from the field and finds himself on the floor of a horrific detention center. What follows is a living hell of interrogations and relentless mental and physical abuse. Alongside other young detainees, he battles to survive while awaiting his fate to be decided. With execution looking certain, he and three other prisoners will risk their lives and make a desperate and extraordinarily brave move for freedom.
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I love this film, especially the cinematography. The detail was photographs in high quality ground-breaking production. Surviving in ours for ever changing complex world.Published 23 months ago by john


text:
Superchips Cortex
3 Attachment(s)
I finally decided to make a purchase for my truck today since it has been awhile since I've done anything to my truck. I got the Superchips Cortex, pulled it out of the box to update it just to discover it's not supported by Mac. Fail! I'm thinking about going ahead and installing it on my truck to play around with it until I can update the software, and to help burn off the 87 in my tank until I get a couple of tans of 91 through the truck. I'm gonna upload some pictures just for a few laughs. The first is of the Cortex in the box, the second is an upgrade that I bet not many of you guys have (or ever want to have lol), and the third is to prove how fuel efficient these 5.7 Hemis really are!
I went ahead and installed the 87 tune on my truck before I had to head to work. It put the biggest smile on my face when I got on the on ramp for the interstate! I can already tell the difference in how the transmission shifts. I never thought I'd be able to bark the Nitto Terra Grapplers going into second gear on the on ramp! I have the feeling my MPG are going to go down for awhile until I get done playing with it lol. I was wondering if anyone knows how big of a file the software update is because I can use our windows based PCs at work to update it. We have a limited amount of bandwidth we can use, so I don't want to drain it before the week is up.
Get the trans upgrade if you really want to get the most from the tuner.
Does the trans upgrade have preset tunes? I'm not the most mechanically sound person, so I don't want to start playing with things too much and trash my transmission. I can usually tackle most of the wiring of electrical stuff, but when it comes to how things run I usually stay away lol
The trans upgrade does not have preinstalled tunes. You need to set the shift points and tm to meet your needs. If you get the upgrade and want a set of base values to start with let me know. I think I still have some stored on my computer from when I did beta testing for superchips.
Congrats on the upgrade. Got mine last week and have my first tank of 91 in her already LOL. I only have the 87 tune in right now however even that is a big improvement over the stock tune.
Not sure how big the updates are, but when I updated mine it took about 5 minutes to download...
Ok that's definitely good to know! Looks like I'll be updating that tonight! Should I return it to stock before trying to get the update and installing it? Also, will it mess anything up if I have to use 4WD? Just trying to make sure I don't mess anything up since I have to go down some rough roads sometimes with my job.
I can't believe that an update will wipe out your stock file but with computers you never know. To be safe I would return your truck to the stock program before updating the Cortex. I have not tried 4WD with the tune on however can't imagine there would be any problems. Maybe just let us know. If you do have problems you can always put the stock program back on, takes only 10 mins.
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Chelsmore Apartments
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The Journey to Vegas
... bitt racist. Food for thought - but I spent much of my time wondering am I Racist or was this a group of arseholes. We landed and then I was in Vegas. This place is large. I checked in to the MGM Grand. This hotel is the first f the big hotels on the "Strip". You join a line – there were so many people – it took 30 minutes to check in, but I had a room despite arriving early. ...
New York, New York - final stop
... the news of our engagement to another person, which was nice. Later we went to Coney Island, the seaside out in Brooklyn where I'd always wanted to go. We had some fantastic posh street food at a Smorgesboard which had just opened, sort of posh little food van versions of popular indy restaurants from the city - an arty, foody heaven. We also walked along the busy boardwalk soaking up all the people and the lovely weather. Coney Island is a ...
Gotham City at its best
... except where we are). You can already see a lot being done up and sold off in a way this is good as the architecture will be maintained but its going to change the feel of the neighbourhood and in all likelihood price the people who have lived in these areas all their lives out of the area which will be a pity.
One of the obvious things about Harlem is the number of churches. This is where you come to see Gospel in action. You can do ...
First day in New York
... him so we did some more shopping, ending up at Macy's, successfully getting gifts for Pam and Valerie.
Back to Greenwich Village and to the same pub as before, for a drink, then back to the hotel for a nap before going out to dinner. We took a recommendation from Jean-Pierre and went to the Blue Water Grill in Union Square. I have to say this was a disappointment. While the food was good,little else was. For a ...
Philadelphia, PA: Day 1
... one was injured! It taught mum a lesson though! That was also the second stack of the day; dad was also over-confident early on and tried to be fancy/silly, and nearly came a gutser. Ryan and I - aced it, naturally! We caught the bus back to our hotel which was a little way out of downtown, and went to the bar to catch the last 15 minutes of happy hour. Cheap beers, tired and hungry Aussies in unfamiliar territory leads to an eventful night! We learned mum is a cheap ...


text:
New York—Kenneth Cole Productions today reported that it swung into a second quarter loss due to a sales decline and mounting costs.
For the quarter ended June 30, the company posted a net loss of $3.7 million, or 20 cents a share, compared with a profit of $579,000, or 3 cents a share, a year ago. Excluding expenses relating to a new distribution center and costs associated with founder Kenneth Cole's purchase of the company, adjusted loss would be 3 cents a share.
In June, Kenneth Cole, the company's founder, chairman and chief creative officer, struck a deal to buy stock he doesn't already own for $15.25 a share, about a $245 million deal.
Total revenue declined 5% to $97.3 million. Revenue at its wholesale division edged down 3% to $50.3 million primarily from private label footwear and Reaction handbags.
In its direct to consumer business, Kenneth Cole posted a 6.1% sales decline to $37.2 million as of its stores were closed. Comparable store sales declined 2.7%.
Licensing revenues declined 7.8% to $9.8 million as the company took its women's apparel business in-house and reset the contractual minimum royalties of a licensee.
Once Kenneth Cole's buyout is completed, Kenneth Cole Productions will be a private company again.
For commercial purposes, extract supplements can be obtained from the fruit of the pericarp. Garcinia cambogia reviews is performed by a physician Chen and Oz to prove that it is an herb for weight loss, a lot of research has been done. Studies, Garcinia cambogia extract supplementation decreases the weight of the body fat accumulation, was shown to have a positive effect on prevention. We are important substances such as cholesterol (LDL), reduces the serum leptin and triglycerides. In addition, it will increase the level of serotonin and cholesterol (HDL). The study also, Garcinia cambogia extract supplements, has proven that there are no significant adverse effects on many of the eight weeks, such as its use.


text:
I used your plugin, and put the short code into my "Welcome" page. It did not work so I went into "editor" and put it in there. That's when I got shut out of my website. I can't log in, I don't have access to my website, there is nothing I can do, I am stuck. This is the message that is given when you go to the site:
Parse error: syntax error, unexpected '<' in /home/content/38/8657138/html/wp-content/themes/twentytwelve/functions.php on line 451
Website: http://www.weddingexperiencepdx.com


text:
By ajcolores
Posted in IndustryMusic | Tags : Arizona, gadsen, gadsen records, indie, music, Phoenix, Record Label
Freddie Gibbs links up with DJ Drama to present his new mixtape Baby Face Killa. Guest features from Young Jeezy, Jadakiss, Kirko Bangz, Dom Kennedy, Jay Rock, Curren$y and more.
Track list and download link below.
Download: Link


text:
H! by Henry Holland Designer black studded biker jacket
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text:
'Words' spells minor disaster
Language of origin? A little too much raunch
Grade: C+
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Sony Ericsson Xperia Play lands - with 60 games
Yes, there are reports of shortages and yes, we know that it's not the fully branded PlayStation phone that the rumour mill has talked about for years - but the Sony Ericsson Xperia Play is still a big deal and it has just gone on sale in the UK.
And, for a mobile phone with gaming at its heart your first question is bound to be: "What games can I play on it?"
Well, luckily Sony Ericsson has detailed the launch range and it's a mix of original PlayStation games, existing "3D titles" and "popular casual games all optimized for the unique game pad". The phone giant has also stated that there are 20 developers on board "from big publishers to independents, from middleware providers Havok and Unity to engaging Android developers".
But we still haven't answered your question really, have we? So, here goes - this is a list of all the games that are available, or will be available within a week:
Bruce Lee Dragon Warrior from Digital Legends (pre-installed)
FIFA 2010 from EA Mobile (pre-installed**)
Sims 3 from EA Mobile (pre-installed**)
Star Battalion from Gameloft (pre-installed**)
Tetris from EA mobile (pre-installed**)
Asphalt 6 from Gameloft (free to download)
Dungeon Defenders: Second Wave from Trendy Entertainment
Galaxy on Fire 2 from Fishlabs
Assassins Creed from Gameloft
Need for Speed SHIFT from EA Mobile
Worms from EA Mobile
Nova 2 from Gameloft
Modern Combat Black Pegasus from Gameloft
Guns n Glory from Handygames
Reckless Racing from Polarbit
Real Football 2011from Gameloft
Gun Bros from Glu Mobile
Racer Luma from Arcade/Unity
Cordy from SilverTree Media
Aporkalypse from Handygames
Brothers in Arms Global Front from Gameloft
GuerrillaBob from Angry Mob Games/Unity
Let's Golf 2 from Gameloft
Zenonia 2 from Gamevil
Cyberlords from Handygames
Guitar Hero™ Warriors of Rock Mobile from Glu Mobile
Age of Zombies from Halfbrick Studios
Spectral Souls from HyperDevBox Japan
Splinter Cell Conviction from Gameloft
Air Attack from Art in Games/Unity
Armageddon Squadron from Polarbit
Uno from Gameloft
InfeCCt from Handygames
Spiderman total Mayhem from Gameloft
Millionaire City from Digital Chocolate
Avatar from Gameloft
Raging Thunder 2from Polarbit
Homerun Battle 3D from Com2us Games
Aftermath XHD from Jakyl
I Must Run from GameLion
Baseball Superstars 2011from Gamevil
Ground Effect Pro from Jakyl
ZeNonia from Gamevil
Super KO Boxing 2 from Glu Mobile
Farm Story from Team Lava
Wave Blazer from Polarbit
Soccer Superstars from Gamevil
Battle Bears from Skyvu
Tower Blocks New York from Digital Chocolate
SuperDynamite Fishing from Handygames
Iron Sight from Polarbit
Toonwarz from Polarbit
Restaurant Story from Team Lava
Escape from AMA
Raving Babies from AMA
Bakery Story from Team Lava
Denki Blocks! Deluxe from Jakyl
Rollercoaster Rush from Digital Chocolate
Crash Bandicoot® from Sony Computer Entertainment Inc.
Cool Boarders 2 from Sony Computer Entertainment Inc.
Destruction Derby from Sony Computer Entertainment Inc.
Jumping Jack Flash from Sony Computer Entertainment Inc.
MediEvil™ from Sony Computer Entertainment Inc.
Syphon Filter™ from Sony Computer Entertainment
Happy gaming.
See also - Sony Ericsson Xperia Play: the games
And - Sony Ericsson Xperia Play vs iPhone 4
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- EE Pocket-lint Gadget Awards: How the voting works
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- Microsoft Lumia 950 vs Lumia 950 XL vs Lumia 930: What's the difference?
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Fr. 897a $10 1914 Red Seal Federal Reserve Note PCGS Extremely Fine 40PPQ.. ...Click the image to load the highest resolution version.
Serial Number One Atlanta $10 1914 Red SealFr. 897a $10 1914 Red Seal Federal Reserve Note PCGS Extremely Fine 40PPQ.
Our landmark sale of seven serial number 1 Red Seal Federal Reserve Notes in our May Central States sale brought forth this hitherto unknown $10 serial number 1 Red Seal from the Atlanta district, which comes to us from the estate of M.B. Wellborn, the first Governor of the Atlanta Federal Reserve Bank. Maximillian Bethune Wellborn was born in Lewisville, Arkansas during the Civil War and raised and educated in Eufaula, Alabama before moving to Anniston around the turn of the century. As a young man he became general manager of the Alabama Frog & Switch Company, a railroad supply company, and then entered the banking business, founding banks in Jacksonville and Piedmont before becoming President of the First National Bank of Anniston in 1905, serving in that position until 1914, when he was appointed the first governor of the newly established Federal Reserve Bank of Atlanta. It was in that capacity he obtained this lovely note, which is accompanied by a handwritten notation on the front of a Federal Reserve Bank of Atlanta envelope which states "The first of note issues of Fed R Bank of Atlanta, No 1...Property of M.B. Wellborn, Nov 1914." Wellborn served as Governor of the Atlanta Fed until 1928, and lived to the age of 95 before passing away in 1957.
Prior to our May sale only two $10 serial number 1 specimens were reported, with only one, the number 1 from the New York district, in private hands. That example has not been seen since its last appearance in 1998. The Chicago district number 1 is in the hands of the Chicago Federal Reserve Bank. After that sale, the number in private hands is now three, with each in very strong hands. Like the Dallas number 1 Red Seal we sold in May, this piece has clearly never circulated, but was folded for storage in the envelope that accompanies the note. The colors, margins, and overall appearance are as sharp as the day the note left the press, with PCGS awarding this note its coveted Premium Paper Quality designation, and our estimate of this number 1 beauty along with its unquestioned provenance may well prove conservative in the extreme this evening.
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Book your room at Die Wasnerin G'sund & Natur Hotel
Get an overview of this hotelFamily-friendly hotel with full-service spa and free breakfast
This family-friendly Bad Aussee hotel is located in the mountains, 1.4 mi (2.3 km) from Kammerhof Museum, and within 9 mi (15 km) of Kammer Lake and Loser Ski Resort. Dachstein Ice Cave and Gjaid Cable Car are also within 12 mi (20 km).
Hotel Features
This smoke-free hotel features a full-service spa, a restaurant, and an outdoor pool. Free buffet breakfast and free WiFi in public areas are also provided. Additionally, a health club, a bar/lounge, and a poolside bar are onsite.
Room Amenities
All 88 rooms feature thoughtful touches like bathrobes and slippers, along with free wired Internet and balconies or patios. Guests will also find sofa beds, cable TV, and minibars.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
In addition to a full-service spa, Die Wasnerin G'sund & Natur Hotel features an outdoor pool and a health club. The hotel offers a restaurant. Guests can unwind with a drink at one of the hotel's bars, which include a poolside bar and a bar/lounge. A complimentary breakfast is offered. Public areas are equipped with complimentary high-speed wired and wireless Internet access.
This luxury hotel also offers a children's pool, a spa tub, and a sauna.
Die Wasnerin G'sund & Natur Hotel is a smoke-free property.
- 24-hour front desk
- Arcade/game room
- Babysitting or childcare
- Bar/lounge
- Pool umbrellas
- Children's club
- Children's pool
- Conference space size (feet) - 130
- Dry cleaning/laundry service
- Elevator/lift
- Express check-in
- Express check-out
- Fireplace in lobby
- Free breakfast
- Free WiFi
- Free wired high-speed Internet
- Full-service spa
- Gift shops or newsstand
- Health club
- Laundry facilities
- Library
- Meeting rooms
- Multilingual staff
- Number of floors - 4
- Outdoor pool
- Poolside bar
- Porter/bellhop
- Restaurant
- Safe-deposit box at front desk
- Sauna
- Shopping on site
- Ski storage
- Smoke-free property
- Spa services on site
- Spa treatment room(s)
- Spa tub
- Steam room
- Television in lobby
- Total number of rooms - 88
- Tours/ticket assistance
- Wedding services
Family Friendly Amenities
- Arcade/game room
- Babysitting or childcare
- Children's pool
- Cribs/infant beds (complimentary)
- Free Wi-Fi
- In-room childcare (surcharge)
- Laundry facilities
- Outdoor pool
- Sofa bed
Available in all rooms: Free wired high-speed Internet
Available in some public areas: Free WiFi , Free wired high-speed Internet
Room Amenities
- Balcony or patio
- Bathrobes
- Blackout drapes/curtains
- Cable TV service
- Free toiletries
- Connecting/adjoining rooms available
- Free cribs/infant beds
- Daily housekeeping
- Desk
- Free wired high-speed Internet
- Hair dryer
- Hypo-allergenic bedding available
- In-room childcare (surcharge)
- In-room safe
- Iron/ironing board (on request)
- Minibar
- Private bathroom
- Room service (limited hours)
- Separate bathtub and shower
- Slippers
- Sofa bed
Where to Eat
A complimentary hot/cold buffet breakfast is served between 7:00 AM and 11:00 AM.
Die Wasnerin G'sund & Natur Hotel has a restaurant serving breakfast, lunch, dinner, and light fare.
Room service (during limited hours) is available.
Nearby Things to Do
A health club and complimentary bicycles are featured at the hotel. An outdoor pool, a children's pool, and a spa tub are on site. Other recreational amenities include a sauna and a steam room.
The recreational activities listed below are available either on site or nearby; fees may apply.
- Free bicycles on site
- Yoga classes/instruction on site
- Downhill skiing nearby
- Cross-country skiing nearby
- Snowboarding nearby
- Rafting nearby
- Windsurfing nearby
- Mountain biking nearby
- Parasailing nearby
- Kayaking nearby
- Sailing nearby
- Scooter/moped rentals nearby
- Hiking/biking trails nearby
- Skydiving nearby
- Aerobics on site
- Golf driving range on site
- Golf lessons on site
- Snowshoeing on site
Spa Services
The onsite spa has massage/treatment rooms. Services include massages, facials, body wraps, and body scrubs. The spa is equipped with a sauna and a spa tub.
- Sauna in spa
- Spa tub in spa
- Massages
- Manicures and pedicures
- Body wraps
- Body scrubs
- Body treatments
- Facial treatments
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- In-room accessibility
- Accessible bathroom
- Accessible path of travel
- Accessible parking
Hotel Policies
Check-in time starts at 4 PM
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Free cribs (infant beds)!
Pets allowed
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- A tax is imposed by the city: EUR 2 per person, per night. This tax does not apply to children under 16 years of age.
- New Year's Eve gala dinner fee
- Resort fee
Optional extras
- Pet fee: EUR 15 per night
We should mention
Reservations are required for massage services and spa treatments. Reservations can be made by contacting the hotel prior to arrival, using the contact information on the booking confirmation. A resort fee is included in the total price displayed. A fee for a mandatory New Year's Eve gala dinner is included in the total price displayed for stays on 31 December. The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
National Rating
Hotelstars Union assigns an official star rating for properties in Austria. This property is rated 4 star Superior and is displayed on this page as 4.5 stars.


text:
[ Posted Monday, August 25, 2003 ]
Party Photos: Cocktails in Los Angeles
At the Bigfoot Lodge, 08.13.03
Jesse, our editor, may have been trying to get out when he went
west on vacation recently, but mb, of course, kept pulling him back in. He didn't
mind: He got to host a fabbo cocktail party at L.A.'s wacky (and un-air-conditioned!)
logcabin lounge. He got to hang out with all the cool kids, have some tasty
cocktails, and even have a run-in with Smokey the Bear.
Learn more
about AvantGuild, our premium membership service. Click here
to receive invitations to mediabistro.com events in your city.
(Click for a larger
view of the pictures.)
Geoffrey Rose, author of upcoming book Let's Go, and Constance Whitelaw, co-owner of Conscious Colors and a freelance writer.
Freelance writers Richard Horgan and Gerri Miller.
Rachelle Kuchter, an asstistant editor at Trade Show Week and Caltech grad student Dylan Nieman.
Stuart Volkow, Pulse Entertainment's Spence Bovel, and Media Revolution's Paul Vandekamp.
Freelance writer Kai Stevens and record-label owner Maria Amador.
Whitney Friedlander, who's in book publishing, and Jennifer Stuart, who's innatchthe film industry.
Entertainment Tonight editor David Weiner and freelance writer Theresa Buyikian.
Freelance writers Mica Campbell and Mike Heavers with Primedia's Steve Kedrowski and Dave Druse of Leisure Publications.
Freelance writers Steven Hanna and Lauren Horwitch.
VirginMega.com managing editor Kim Taylor with Carrie Borzillo-Vrenna, senior editor and sex columnist at Gene Simmons' Tongue.
Freelance graphic designer Ken Wessel, freelance writer Sandra Matke, and Metro Mag's Leslie Davis.
Writer Kate Nielson, mb's Jesse Oxfeld, and Jesse's friend Dylan Nieman.
mb's Jesse with creative director Jill Whysel.
Orange Coast managing editor DeAnna George and editor Tina Borgatta with freelance writers Nelson Handel and Greg Cohn.
Photographs by Jill Whysel.
Pitch hard-hitting stories affecting girls and women to the grand dame of feminist reads.
This long-running publication wants scientific pitches with a strong news hook.
Pitch narrative stories that upend conventional wisdom about global politics, economics, people and power.
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text:
Oklahoma football: A platoon might clear up quarterback picture
Stoops' pal Steve Spurrier went all Tom Landry against top-ranked Florida State in 1997, and Florida pulled off a 32-29 upset as Doug Johnson and Noah Brindise shuttled in and out almost every snap.
But that was for strategic reasons. Stoops doesn't need a strategic infusion. He needs clarity.
And clarity comes on Saturdays.
That's not the situation Mike Gundy faces. In Stillwater, Gundy has two quarterbacks who have faced the fire. Both Clint Chelf and J.W. Walsh (and Wes Lunt, before his transfer) have quarterbacked multiple Big 12 games, to success. Which means Gundy has plenty of data and eyewitness accounts to make a decision. Gundy knows what he has.
Stoops does not. Stoops' quarterbacks have been on the driving range and the putting green. They have yet to stand on the tee box.
The same thing occurred in 2005, when Paul Thompson and Rhett Bomar battled for the job in August. Stoops announced Thompson as the starter but said both would play against TCU in the season opener. Both did play — poorly, and OU lost 17-10. Bomar started the next week and kept that status the rest of the season.
Stoops said he resorted to a planned platoon in that 2005 season opener "just to get a feel for both of 'em, because a lot of times, we like both of 'em or feel both of 'em are capable of winning and sometimes don't feel like at that point, where neither one has played a whole bunch, they've got a chance to help take some pressure off the other guy."
Again, I'm not advocating a platoon system. I'm calling for platooning until the quarterbacks decide the race for themselves, which is always the preferred method.
If takes a game or two or even three, so be it. When the gauntlet of Notre Dame (Sept. 28), TCU (Oct. 5) and Texas (Oct. 12) arrive, Stoops needs to know who is his best quarterback, instead of just hoping he made the right decision.
Berry Tramel: Berry can be reached at (405) 760-8080 or at email@example.com. He can be heard Monday through Friday from 4:40-5:20 p.m. on The Sports Animal radio network, including FM-98.1. You can also view his personality page at newsok.com/berrytramel.


text:
Enjoy PayPal on-the-go with the PayPal Windows Phone app. SEND & REQUEST MONEY Whether you want to send money as a gift or pay back a loan from a friend – sending money is easy and FREE (when using a bank account or PayPal balance as the funding source) MANAGE YOUR ACCOUNT Check your balance, withdraw funds or view past transactions – anytime, anywhere ADDITIONAL FEATURES • New "Local" feature where you can find local merchants who are using our PayPal Here™ service and pay them with PayPal. • Online or on-the-go, your financial information is safer and more secure with PayPal. Please note: Your country may not support all features.More
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1-10 of 877 reviews
Very good app to control your PayPal account. I missed just a transparent tile.More
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Very basic app with limited functions, often inconsistent and no way to request help when it fails. Childish attempt.More
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Trying to sign up for an account but at the very end it tells me that there is not internet connection. I have tried this on two different phones with the same problemMore
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Didn't work at all. Logged in, hit the check mark button, and it closed down...More
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Not as good as Android app that I used to have but at least I can still keep track of my balance and perform basic tasks. Desparately need PayPal HERE app since I can no longer scan credit cards on my new Windows Phone. Business is really suffering without PayPal Here's card scanning ability!More
- Yes (1)
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Three things are wrong/missing. 1. We need PayPal HERE. Period. 2. For security, the app should log out/lock on close. 3. I can't log in with my new PIN.More
- Yes (3)
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Allows me to manage my PayPal on the go, and it's a real WP-specific app rather than a half-baked port.More
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A quick reskin for the updated PayPal branding/design. Would like to see a whole app make over keeping it in line with the other mobile platforms. Also the tile icon seems to be low resMore
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Like it but it can be much betterMore
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It's alright, I guess. Can't view tracking info on eBay purchases. Transaction info is very limited. When I withdraw money to a bank account I wish the app would show the name of the bank I'm transferring to instead of just the account #. Purchase history is sometimes duplicated and displayed in a strange way. The app really needs some work.More
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1-10 of 877 reviews
App details
- Publisher
PayPal Inc.More
- Category
Personal FinanceMore
- Approximate size
4.34 MB - 5.33 MBMore
- Age rating
For All AgesMore
- Supported processors
- Supported languages
Português (Brasil)Dansk (Danmark)English (Australia)English (United Kingdom)Français (France)Nederlands (Nederland)English (United States)עברית (ישראל)中文(中华人民共和国)日本語 (日本)Русский (Россия)Italiano (Italia)Norsk, Bokmål (Norge)Polski (Polska)Deutsch (Deutschland)Svenska (Sverige)Español (España, Alfabetización Internacional)中文(香港特別行政區)中文(台灣)
- Learn more
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- More
- Capabilities
Use your contactsUse an anonymous Microsoft accountUse your locationUse your musicUse the photos in your media libraryUse the media items that are currently playingUse your device network servicesUse your phoneSend push notificationsAccess your browserUse information about your deviceUse your media library
Version notes
188.8.131.52 Update - Update to the payment code functionality to support more retail locations


text:
I recently reported on how, fed up with both day after day of nasty newspaper headlines and one civil society leader after another abandoning them, Accra's gay community came out, one-by-one, at a September 30 major 'straight' forum.
The hosts of the forum, ACCRA [dot], a monthly film and discussion series in Ghana, "where the dopest artists and creatives meet over good cinema, talk, food and music," have now produced a video of what one gay Ghanian blogger described as a "remarkable" night.
Over the past few months, Ghana's President Mills, preachers and Parliamentarians have made vigorous speeches about homosexuality being ungodly and un-African. In contest to these public statements, September's Talk Party explored life in Accra for young gay men.
These men (from the Coalition Against Homophobia in Ghana) spoke proudly of their sexuality and answered questions from the curious crowd about the challenges of being gay in Ghana.
The Talk Party Series: Gay in Ghana from REDD Kat Pictures on Vimeo.
Related Stories:
Gay Ghanians Come Out as Civil Society Abandons Them
Ghana Official Orders Gays and Lesbians "Rounded Up," Arrested
Will the UK Deny Aid to Anti-LGBT Countries?
Read more: africa, africa lgbt, ghana, video
Photo credit from the REDD Kat Pictures video
is not good oooooooooo
quick correction, the forum's called ACCRA [dot] ALT rather than ACCRA [dot].
Apart from that, I salute these guys' strength and bravery... it's so hard to walk around hearing them bashing on the radio and in the newspapers every single day. I just hope and, yes, pray that we can find a way to open minds.
They're so brave!
What bravery..names and faces in this culture..i'm shocked and happy, but pray for their safety.
Thank you.
Being homosexual is one thing, accepting it as normal or desirable is another. Mind you we can expect nothing else from those who use the Gay community as propaganda to further their depopulation and anti family and reproduction agenda. Mark my words all this Gay propaganda is going to get us in deep water with no paddle to get us out of it. I never hear anyone say Love they neighbour as theyself and apply to Gays as well, but that does n,t mean we must accept it as normal. Normal it aint, and never will be. Just like someone who is born without arms or legs ,it's a pity but it's not normal. We are meant to love them as well.
Thank you for taking the lead!
They ought to round up Ghana's government officials and prosecute them for theft from the public, bigotry in the form of homophobia, and impersonating a government. The gays would do a far better job of running this country.
Congratulations to these people for their courage by coming out at great risk to their freedom and lives both from criminals who would attack LGBT people against the law and from the legal system that has chosen to criminalize people because of their sexual orientation.
Wonderful. Thanks Paul.
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grow big and strong budi. i hope you have a wonderful life.
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Republicans in Washington struggled to fill a leadership void on Thursday as the front-runner to take control of the House of Representatives, Kevin McCarthy, quit the race in a surprise announcement that heightened concerns about the party's ability to govern effectively. Representative McCarthy, the No. 2 House Republican, had been expected to win Thursday's contest for the nomination to succeed retiring Speaker John Boehner despite opposition from more conservative lawmakers demanding a harder line against Democratic President Barack Obama's agenda. "For us to unite, we probably need a fresh face," McCarthy, who is from California, told reporters after the election meeting ended in chaos.
A growing awareness of how the Ebola virus can hide in parts of the body such as eyes, breasts and testicles long after leaving the bloodstream raises questions about whether the disease can ever be beaten. Virologists said Friday's case of a Scottish nurse, Pauline Cafferkey, who had recovered from Ebola but is now suffering complications adds to signs that the virus is a long-term health risk and can lead to a "post-Ebola syndrome". "Over the past few years there has been mounting evidence of mental and physical health problems in Ebola survivors that can last for years after the virus is cleared from the bloodstream," said Ben Neuman, an Ebola expert and lecturer in virology at Britain's University of Reading.


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The Atherton Hotel at OSU
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Travel Blogs from Stillwater
Finally a home for a few nights
We stopped in Oklahoma for the weekend to spend time with Jenny and Camron (Tj's sis and bro-in-law) ...Zoe is so happy to be in a home around people who love her and not just a hotel! Camron is helping her play the piano and I think that is her new favorite hobby! We went to see a movie (Tommorowland was amazing)
Dogs are doing great
Cat is great
Carthage to Chandler
... into Oklahoma (yes Route 66 only lasts for 13 miles through Kansas). The first place we stopped was to visit the Worlds Largest Totem Pole which was nice, in the summer we imagine it would be a great place for picnics. Near there was Annies Diner where we had a couple of shakes and spoke to Annie about our journey. Her opinion was that Arizona is one of the best places on the route. We took a few peices of advice from here about places to stay ...
Guthrie OK is worth the stop
We took the car into Guthrie this morning and went through the Territorial Museum, which told about the relocation of indian tribes, the "land grab, and early life on the prairie and the hardships endured.
Government-owned property (once indian territory) was surveyed out in 160 acre tracts and at 12:00 noon on a day in August, the area was "opened" for whoever could stake a claim. Trains were packed with people who were kept on the train until ...
Interesting Wild West!
We visited the town of Guthrie this morning. They have designated the whole area as historical district, so being downtown was like being in an area during the turn of the century. All of the buildings had been restored, and the architecture was very interesting. We went through the Oklahoma Territorial Museum which told the history of the relocation of the ...
Oklahoma is O.K.!
We started out this morning from McAlester, Ok, made the trip up to Emporia, Kansas, and arrived this evening at Guthrie, O.K., not too far from where we started.
Come back tomorrow night ( if I have internet connection) and I'll tell you where we went and what we did.


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September 7, 2011
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I only like to fuck that pink scarf.
I like this movie...
That song is going to be my new ringtone
Today, girls get fucked in porn videos. Those days, the girls fucked the guys!
those videos are very nice...i like them


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Super small and friendly and warm guesthouse. Very cheap and Mat, the owner is very great and devoted to his gest. Very well located to eat and to catch taxi to go towards the Perentian islands. I strongly recommend this place
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- Timur Guesthouse Kota Bharu, Kelantan


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Does not nature itself teach you that if a man wears long hair it is a disgrace for him, (1 Corinthians 11:14)
Why is Jesus often depicted in paintings with long hair, if it's disgraceful for men?
Why is Jesus often depicted in paintings with long hair, if it's disgraceful for men?
Even if the premise that Christ had longer hair is true, it would not matter as that is not the point of this passage. If it is read in context i.e. read the chapter before and after, it is clear that Paul is talking about how Jewish customs and how even though they are no longer necessary due to Christ's sacrifice, they should still be observed if you are causing your brother to stumble:
Then he goes on in the start of Chapter 11 to talk about the custom of women covering their head to worship and men uncovering their head to worship and why that custom was in place: because angels needed to cover their head out of reverence to God. I'm not a Jewish custom expert but I do know that the point of this passage was not to show that it is disgraceful for men to have long hair but to show that you must respect the customs of the people you are around in church. This is shown by the ending phrase of that section:
It is important to always read God's word in context as it was meant or else you run the danger of missing the point.
Hope this helps :)
Being as there is no image of Jesus that has any historical credibility, the question is based on a faulty premise. We simply do not know what Jesus' hair looked like.
The paintings to which you allude tend to be those painted by the European masters in the 16th & 17th Centuries. At the time, it was fashionable for men to have longer hair, and thus it was not "a disgrace".
It is important to remember that Jesus is God's Word in the flesh, thus an eternal being. Although He entered into human consciousness during a period in history, He did not live in that period alone. The Son of God was always present even before the foundations of the earth and forevermore. If Jesus were portrayed with a certain haircut, it would put Him in a period. The portrait of Jesus cannot be presented with a hair-style or a haircut because He is an eternal being: the same reason why angels always have long hair.


text: With some of my colleagues I came for a conference in USF and stayed here. I got a spacious room (stayed alone) and bed is quite comfy. I got the room rate around USD 59 and its more than expected for that amount. I stayed in another hotel in Florida for USD 169 (shared with friends) and it can be compared to this place; and we didn't get breakfast.The staff is very friendly. The free breakfast quite ok and bit dull, but I dont think we can expect much in a continental breakfast. My room was next to gym and it is the frist room in ground floor, there was no disturbance (also close to lift and I only noticed it last day).


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After snapping-up a bushel of Brooklyn agents over the past year or so, Compass — the design-and-technology-conscious real estate upstart — is officially opening two offices in Brooklyn.
The Park Slope office will be at 514 2nd Street, just a stone's throw from the brokerage-blooming stretch of 7th Avenue running roughly from 5th Street up to Berkeley Place. We're awaiting information on the location of the Williamsburg office.