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The newest pseudo-candid queen Nathalia Ramos was at the beach the other day, playing ping pong of all things in a bikini with her inconceivably perfect little body. She looks like she's in a Noxzema ad from the 90s in these, just having a grand old time laughing and smiling with her perfect skin, playing in the sun and being hot. All you need is some Color Me Badd playing in the background and you'd have the essence of the typical 90s after school TV commercial break moment come back to life. I wanna grab some Surge and fruit roll-up and watch a Fresh Prince marathon. That was a weird place to go in my mind.
Click on each photo to enlarge!


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Emerging from the covers like an overdue baby. He is going to look so cute in diapers.
Posted in Daily Chuck | Tagged Chuck, dog
Burial Ground
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Contemplative Coco
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Bright "Indienne " cotton poplin print. Sold by the yard.


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Due to continued rain in Tuscaloosa, Tuesday's game between No. 9 Alabama and UAB has been postponed and rescheduled for Wednesday, April 9, with first pitch scheduled for 6:05 p.m. CT.
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Alabama and UAB Baseball Game Postponed until Wednesday
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Aggravated Assault Suspect Evades Garland PoliceThe SWAT team made numerous attempts to reach out to Randall. They even used tear gas to bring him out the home but they later realized he'd gotten away.
Standoff In Ft. Worth Ends With Suspect Jumping Out Of WindowA Fort Worth Police SWAT team arrested a man who barricaded himself in an apartment complex after allegedly critically shooting a woman multiple times Saturday morning.
Standoff With Felony Suspect EndsA man wanted on four felony warrants and evading arrest barricaded himself inside a home on E. Kellis Street for about three hours.
Arlington Standoff Ends With SurrenderA man accused of threatening a UT-Arlington student is in custody after a four-hour stand-off.
Man Barricaded In Mansfield Home, SWAT StandoffA loved one of the man called police saying an unstable family member had barricaded himself inside the home Friday afternoon.
Hours Long Fort Worth Standoff Ends Without IncidentA standoff in Sansom Park has ended with police taking the suspect into custody without incident.
Pizza Delivery Leads To Irving StandoffA police standoff in Irving ended after nearly four hours with the suspect being taken into custody. And it all started with a pizza delivery driver's determination to get paid.
Dallas Police Discover No Suspects After SWAT Situation In LakewoodThe Dallas Police Department SWAT team was involved a suspected standoff in the Lakewood area. But after more than three hours police confirmed that they approached vehicle, but found no one inside. No arrests were made.
Mesquite Police Standoff Ends Peacefully Police closed both the east and westbound lanes of Highway 80 at Collins Monday afternoon in Sunnyvale due to a standoff with an armed man.
Police Standoff Closes Richland Middle SchoolAuthorities are involved in a standoff on Tuesday morning at a home in Richland Hills. The scene is located less than a mile away from Richland Middle School.
Standoff Ends Peacefully In Haltom CityA standoff ended peacefully in Haltom City on Wednesday after a car stolen out of For Worth was spotted in the area.
Dallas Police Standoff Ends PeacefullyA police standoff in Dallas ended peacefully on Thursday morning after seven hours. An armed women who reportedly had mental issues refused to come out of a home.


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Prescott sticks by criticism of schools planJohn Prescott yesterday stood by his outspoken attack on a key element of the government's flagship school reforms as the ripples caused by his remarks threatened to cause deeper divisions in the Labour ranks.
Legal review will mean fewer murder chargesThe first comprehensive review of the law of murder for more than 50 years will recommend a fundamental change that would see many killings downgraded to manslaughter, the Guardian has learned.
Front page
Beleaguered Bush steps up PR blitz with live addressGeorge Bush last night used a live TV address to spell out his strategy for winning the Iraq war, part of an attempt by the administration to shrug off a disastrous summer which saw the president's approval ratings fall to an all-time low. By Jamie Wilson in Washington.
Top p3
Lorry used to steal £3m Moore sculpture found on housing estate· Police fear bronze may be sold for £5,000 scrap value
· Reward offered for return of two-tonne masterpiece
Cherie Booth's role in Cyprus land dispute angers presidentThe president of Cyprus, Tasson Papadopoulos, has condemned Cherie Booth for agreeing to defend a British couple in a land dispute that has become one of the most sensitive issues on the island.
Rooney pipped by God in survey of under-10sForget holidays by the sea, computer games and religion. According to a survey of under-10s released today, "money and getting rich" is the top priority for the nation's children.
National news p5
Adams should resign over spy scandal, urges SDLP· Buck stops with Sinn Féin chief, say rival nationalists
· Republicans speculate real mole is more senior figure
National news p6
The recipe for success: get happy and you will get ahead in life
· Feeling good 'is cause, not effect, of achievement'
· Same principle applies to nations, academics claim
Britain helped US seize pair held in Guantánamo, says ex-ministerBritish agents helped the Americans seize two UK residents and incarcerate them in Guantánamo Bay where they are still detained, a former Foreign Office minister said yesterday.
Family of five killed in house fireChristmas tree lights may have started a house fire that killed a family of five people, investigators said.
National news p7
PC with high arrests tally told to curb his zealNottinghamshire policeman Diederik Coetzee, 48, made a record-breaking 309 arrests this year, once logging 11 in one day. But his superiors told him to be a little less earnest.
Last-minute shopping for presents to top £9bnShoppers will spend £9.58bn on presents in the week before Christmas, £1.91bn more than last year, research suggests.
Two in five check work emails at Christmas break
Even Christmas cannot quell workers' need to check their office emails, a survey reveals today. More than two in five staff plan to log on to their work emails over the festive period, the survey from Fujitsu Siemens Computers showed.
Gatting's bust-up with umpire just wasn't cricket, said British envoyá Messages to Whitehall criticised England team
á Confrontation that halted Test 'reflects badly on us'
Westlife beaten by JCB to top of the pop charts
An unknown folk-pop duo reached the top of the UK singles chart yesterday with a song about a JCB digger.
National news p8
Champagne, pedicures, and a place in history for the Belfast bridesSame-sex couple take first plunge in last place in UK to decriminalise homosexuality.
National news p9
Celebrities pay high price for open justiceMarcel Berlins: In each case someone had told a tabloid of their arrest and there followed massive publicity, focusing of course on the sex lives of the two musicians.
Clerks' strike likely to close many magistrates courtsMagistrates courts in London and 20 other centres are expected to grind to a halt tomorrow because of the first ever strike by clerks and managers, according to a confidential report by Whitehall managers.
National news p10
BSE still lurking in dirty feed binsFarmers have continued to unwittingly give BSE to their own cattle, a government investigation has suggested.
Schools team up to teach unruly pupilsAlmost 300 secondary schools are to set up joint units to teach the most disruptive pupils as part of a package of measures designed to improve classroom behaviour, it emerged last night.
Blair says EU budget rebate will increase· PM claims victory in face of widespread criticism
· Pro- and anti-EU MPs line up to attack deal
Blair considers plan to scrap sleaze watchdog·Body that led to Blunkett's downfall facing abolition
· Appointments committee has clashed with PM
National news p11
Rise in poll complaints troubles Iraq vote monitorsSuspected polling violations on voting day last week far exceeded the number in Iraq's first election in January, local and international monitors said yesterday.
Sharon in hospital after suffering stroke· Israeli leader 'conscious and undergoing tests'
· Health now major issue in looming elections
International news p12
Inquiry begins into work of stem-cell pioneer
Seoul National University launched an investigation yesterday into the work of the leading stem cell researcher Hwang Woo-suk, amid allegations that he faked nine of his 11 stem cell colonies.
Prizewinning author involved in peace talksColombia's Nobel prizewinning novelist Gabriel García Márquez yesterday admitted he has been secretly involved in almost every recent attempt, all unsuccessful, to achieve peace in his country.
King to step down and hand over to son
The monarch of the tiny Himalayan kingdom of Bhutan announced yesterday that he would step down as ruler in 2008 and hold the country's first national elections for parliament.
Bulgakov classic to enthral RussiaThe streets are expected to be deserted tonight at 8.55 when millions switch on their televisions for the first Russian screen adaptation of The Master and Margarita, a surreal 1930s novel that features a gun-slinging cat and the devil as a magician.
City left to shiver as heating system breaksMore than 100,000 people were left without heat and hot water amid subzero temperatures in the Siberian city of Kyzyl after a breakdown at the central heating plant, an emergency official said.
Rampaging Santas take too much Christmas spiritA gang of drunken Santas has caused mayhem in Auckland, New Zealand, throwing bottles, robbing stores and assaulting security guards in protest at the commercialisation of Christmas.
Sharon's aide helps Spielberg promote controversial film
· Israelis condemn movie on 1972 Olympic killings
· Widow of one of 11 dead athletes defends director
International news p13
Gulf states show concern at Iran's nuclear plansGulf Arab leaders yesterday discussed turning the Middle East into a nuclear-free zone amid growing unease over Iran's nuclear intentions.
Bolivian poor glimpse victory for their championBolivians went to the polls yesterday to elect a new president, with popular indigenous leader Evo Morales hoping for a historic victory in south America's poorest country.
Kong fails to grab US filmgoersIts gorilla may be gargantuan and its special effects budget spectacularly huge, but the latest blockbuster movie from America is having problems pulling in cinemagoers in equally enormous proportions.
International news p14
Stampede at Madras relief centre kills 40At least 40 people, mostly women, were killed and more than 50 injured in a stampede for food coupons at an aid shelter in Madras yesterday.
Warlords and women take seats in Afghan parliament· First session of elected body for three decades
· New MPs keen to make progress despite cynicism
International news p15
Unprotected sex pays double, so poverty helps spread of HIVIn Congo, Médecins sans Frontières is encouraging prostitutes and pimps to use condoms.
International news p18
Lamy calls on Blair to broker trade deal
Hopes pinned on fresh talks after failure to deliver but campaign groups say poor have been betrayed.
Financial p20
The world pays a heavy price for our cheap Christmas miraclesMadeleine Bunting: This age of almost disposable clothing is doing huge damage to developing countries and the environment.
Hope at last for schoolsRoy Hattersley: A backbench rebellion should put paid to any plans for a return to the 11-plus in schools.
Debate & comment p23
The nadir of occupationSalim Lone: Last week's election does little to heal the wounds that are splitting Iraq up into violent fiefdoms.
A score draw in EuropePeter Preston: Britain didn't get its own way on the EU budget this time, but Gordon Brown need not despair.
Civil partnerships are divorced from realityPeter Tatchell: Today's rich variety of relationships and lifestyles is not reflected by this new one-size-fits-all legislation.
Debate & comment p24
Cameron's impact means Kennedy and Blair must goJackie Ashley: Labour and the Liberal Democrats both need to change leaders now the Tory party is back as a political force.
Help - send more gunsHester Lacey: The authorities want us to prepare for a disaster, but any fool knows what we will need is firepower.
Debate & comment p25
Corrections and clarifications
Stormontgate's hall of mirrorsLeader: One of the differences between today's jihadist terrorism and yesterday's IRA version, senior police officers have said, is that police have no advance warning about the former, but were often kept well-primed by secret agents about the latter.
Country diaryMark Cocker: Hardley, Norfolk
A bigger achievement than it seemsLeader: The Brussels EU budget summit may not have been anyone's triumph - but that does not mean that it was a disaster - and there is a good argument for seeing it as a solid success for the 25-nation Europe as a whole.
In praise of ... Sir Tim Berners-Lee
Leader: It may seem mundane to others, but to the sleepless fanatics of the world wide web it has been their Princess Diana moment, a catalyst for a worldwide release of affection and emotion.
Leaders and replies p26
Rural idylls and farming realitiesLetters: Simon Jenkins hits the nail on the head (We must help farmers hold back the threat of a rampant suburban sprawl, December 16). Since this year and the new single payment system, the CAP itself no longer subsidises farmers for their output of food.
We will stand firm on our liberal principlesLetters: Martin Kettle writes "centrist" when he really means "rightwing" (A botched putsch by people who yearn to be ministers, December 17). He writes "moderniser" (not a term Liberal Democrats use in internal debate), when he really means "Blairite".
Pointless ivyLetters: Tony Blair supports the redistribution of wealth from rich to poor nations within the EU yet baulks at doing the same in respect of individuals in this country (Blair criticised over EU deal, December 17). Why?
Have some faith in shamans and spiritualityLetters: I fully agree with Margaret Cook about the development of notions of morality being outside of religiosity (Letters, December 17), but she is wrong to talk of so-called high-flyers seizing roles of dominating shamans
Making it like a manLetters: Let me get this straight: Kate Moss is worthy of the same kind of commendation as the McCartney sisters, Marla Ruzicka, Rosa Parks, Mukhtaran Bibi and Rania al-Baz (Those we have loved, G2, December 16)?
Open doorThe readers' editor on ... a 'protected space' for expressions of faith.
Leaders and replies p27
John SpencerObituary: US actor best known for his role as astute political sidekick in The West Wing.
Jon ClarkObituary: An expert in industrial relations active on behalf of the police.
Obituaries p28
Annie DoddsObituary: Documentary maker who gave a voice to Holocaust survivors.
Abdulkadir HersiObituary: Abdulkadir Hersi, better known by his nickname Yam Yam, who has died in a car accident in Columbus, Ohio, aged 60, was an influential Somali poet and playwright whose creative output did not wane following the state's collapse and subsequent civil war.
Edward SzczepanikObituary: The last prime minister of the Polish government in exile.


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Swansea City became the first Welsh club to reach England's top flight since 1983 when they beat Reading 4-2 in an enthralling promotion playoff final at Wembley on Monday.
Scott Sinclair's hat-trick, including two penalties, and a Stephen Dobbie goal earned Swansea victory in a game worth an estimated 90 million pounds ($148.3 million) in increased revenue.
Swansea, who finished third in the Championship behind promoted Queens Park Rangers and Norwich City, led 3-0 at halftime but Reading roared back with a Joe Allen own goal and a Matt Mills header.
However, Sinclair struck his second penalty with 10 minutes remaining to ease te nerves of 40,000 Swansea fans inside the stadium and return the club to the top echelon for the first time since they were relegated in 1983.


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"If you like your health plan, you can keep it" was last year's Lie of the Year. When millions of cancellations stacked up, the game of make-believe was over. So the Democrats, hoping to survive another election cycle, shifted to a new mantra: they claim they want to fix the law. The facts show this is just another Obamacare lie.
The hundreds of thousands of "nonpartisan Obama War protesters" have been found!
"God bless you, and God bless the Homeland."
As ISIS appears to be gaining ground in Iraq, there seems to be a lack of a grand strategy coming out of the White House. The low hum of drone warfare, as opposed to coordinated decisive victories like in Irbil, creates a greater potential for feeding ISIS' propaganda machine. The United States must think more comprehensively than a military intervention.
On the long road to 2016, Chris Christie would be well advised to remember the 1992 Clinton adage, "It's the economy, stupid." Because it's the Jersey economy - not the bridge scandal - that's likely to dog his nascent steps toward the GOP nomination.
I guess the college "Greek system" was always Greek to me.
Get this: Home-cooked family meals are tyrannical.
Politicians write books to establish bona fides, earn money, or posit for higher office. A new one by Kirsten Gillibrand, the popular Democratic senator from New York who once had an A rating from the NRA, seems to have the unintended consequence of shooting its author in the foot.
What if we held politicians to the same standards as football players? When football players break the law, Americans demand they be benched, cut, or suspended before they get due process. But when politicians are indicted they get to stick around, spending our money until a jury of their peers-there's a terrifying thought-passes judgment. It's possible that Americans have misplaced priorities.
Last week, the House and Senate voted to rubber stamp President Obama's war plans for the Middle East. Both bodies, on a bipartisan basis, authorized the US to begin openly training and arming the rebels who have been fighting for three years to overthrow the Assad government in Syria.
Does the "civilized" world have what it takes to defeat the self-declared Islamic State, otherwise known as ISIS?
Thank you for taking a minute from watching the omnipresent anti-war protests to read this column.
Just sit right back and you'll endure a tale...
Domestic violence just doesn't happen on elevators.
On November 30th, voters in Switzerland will head to the polls to vote in a referendum on gold. On the ballot is a measure to prohibit the Swiss National Bank (SNB) from further gold sales, to repatriate Swiss-owned gold to Switzerland, and to mandate that gold make up at least 20 percent of the SNB's assets. Arising from popular sentiment similar to movements in the United States, Germany, and the Netherlands, this referendum is an attempt to bring more oversight and accountability to the SNB, Switzerland's central bank.
Page 35 of 133
What's a teacher to do if he sees a wired briefcase that looks like a homemade bomb in the classroom?
To the one thirteenth of all Americans who watched the latest GOP debate, congratulations on surviving the political equivalent of the 24 hours of Le ...
Is the current refugee crisis gripping the European Union "all America's fault"? That is how my critique of U.S. foreign policy was characterized ...
The Marine Corps just spent $36 million to learn that an average-sized woman is not as big and strong as an average-sized man. I'm ...
I didn't watch CNN's telecast of the Republican debate because I was there in person.
Democrats are bailing on scandal-plagued Hillary Clinton, and the big Democratic Party money can't possibly be enthusiastic about socialist Bernie Sanders.
Apologies are a first world problem. Sometimes, the apology is pro-forma, as in "I am sorry if anyone was offended by my words." Sometimes, an ...
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On October 5, we hosted Dr. Rob Sellers of University of Michigan who spoke on how diversity is an essential component of excellence in education. Read more
Our Fall 2015 Brown Bag Series takes place on select Mondays from 12:00 to 1:15 in the Faculty Commons located in 116 Pearson Hall. Beverages and cookies provided; bring your own lunch. All are welcome. Read more
The Center for the Study of Diversity has released its second annual report, outlining the Center's most recent activities, accomplishments, and goals.
Read it here
The College of Arts and Sciences affirms our commitment to fostering a diverse and respectful educational environment that advances critical thinking, open inquiry and full participation by students, faculty and staff. Read More


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Are you Guido Macchiarelli?Claim your profile
Publications (103)173.51 Total impact
Article: Mancozeb affects mitochondrial activity, redox status and ATP production in mouse granulosa cells[Show abstract] [Hide abstract]
ABSTRACT: Background: Mancozeb (MZ) is a fungicide that belongs to the subclass of metal (Mn/Zn) ethylene-bis-dithiocarbamate pesticides. In mouse and human granulosa cells (GCs) exposed to MZ (0.01μg/ml), morphological modifications and significant alterations of p53 expression level in comparison with control GCs were recorded. Objectives: To investigate if MZ (0.01μg/ml) induces oxidative stress and alters energy metabolism in exposed mouse GCs. Results: Following fungicide exposure, GCs showed low p53 content, a depolarized mitochondrial membrane potential (ΔΨm), as well as low ATP and reduced glutathione (GSH) levels associated with increased reactive oxygen species (ROS) generation. No remarkable differences on other parameters such as ATP/ADP ratio, energy charge, as well as induction of apoptosis and DNA damage were found. The activation of AKT and PDK1 kinases in MZ-treated cells was observed. Inhibition of ROS generation by the antioxidant N-acetylcysteine (NAC) restored a normal expression level of p53. Conclusions: Our results demonstrate that the low dose of MZ here used induces a mild oxidative stress in GCs, and provides evidence for the possible involvement of AKT/PKB signaling pathway in triggering adaptive and survival response.Toxicology in Vitro 09/2015; DOI:10.1016/j.tiv.2015.09.018 · 2.90 Impact Factor
Article: Ultrastructural markers of quality are impaired in human metaphase II aged oocytes: a comparison between reproductive and in vitro aging[Show abstract] [Hide abstract]
ABSTRACT: Childbearing delay contributes to the increase of subfertile couples that require assisted reproductive technology (ART). Subfertility relates with reproductive aging (RA). In vitro aging (IvA) (due to extended culture) may also impair oocyte competence. Aims of this study were to evaluate and compare the oocyte ultrastructure after RA and IvA. Cumulus-oocyte complexes (COCs) (n = 68), with metaphase II oocyte and expanded cumulus, from consenting patients (<35 years old and ≥35 years old, n = 36), were selected by phase contrast microscopy and fixed at pick up, or after 24 h culture. COCs (n = 44) were studied by light and qualitative/morphometric transmission electron microscopy. Two-way ANOVA, with age and culture as grouping factors, was applied for statistical analysis (p < 0.05). Metaphase II cumulus-free oocytes (n = 24) were selected for confocal microscopy observations. Significant decrease of mitochondria-smooth endoplasmic reticulum aggregates, increase of mitochondria-vesicle complexes size and amount, decrease of cortical granules and microvilli, and alterations of the spindle structure characterized both RA and IvA oocytes. These changes were significantly more evident in the RA oocytes submitted to IvA. RA oocytes also showed changes of the zona pellucida and occurrence of vacuoles after culture. Cumuli appeared re-compacted after culture, irrespective of the age of the patients. These data demonstrated that aging is related to decay of oocyte ultrastructural quality, and that oocytes from elder women are more sensitive to prolonged culture (IvA) than the oocytes from younger women. These morphological results should be considered when applying ART in aged patients, rescue ICSI, or artificial oocyte activation.Journal of Assisted Reproduction and Genetics 08/2015; DOI:10.1007/s10815-015-0552-9 · 1.72 Impact Factor
Article: Differences in the Kinetic of the First Meiotic Division and in Active Mitochondrial Distribution between Prepubertal and Adult Oocytes Mirror Differences in their Developmental Competence in a Sheep Model[Show abstract] [Hide abstract]
ABSTRACT: Our aim is to verify if oocyte developmental potential is related to the timing of meiotic progression and to mitochondrial distribution and activity using prepubertal and adult oocytes as models of low and high developmental capacity respectively. Prepubertal and adult oocytes were incorporated in an in vitro maturation system to determine meiotic and developmental competence and to assess at different time points kinetic of meiotic maturation, 2D protein electrophoresis patterns, ATP content and mitochondria distribution. Maturation and fertilization rates did not differ between prepubertal and adult oocytes (95.1% vs 96.7% and 66.73% vs 70.62% respectively for prepubertal and adult oocytes). Compared to adults, prepubertal oocytes showed higher parthenogenesis (17.38% vs 2.08%respectively in prepubertals and adults; P <0.01) and polispermy (14.30% vs 2.21% respectively in prepubertals and adults; P<0.01), lower cleavage rates (60.00%vs 67.08% respectively in prepubertals and adults; P<0.05) and blastocyst output (11.94%vs 34.% respectively in prepubertals and adults; P<0.01). Prepubertal oocytes reached MI stage 1 hr later than adults and this delay grows as the first meiotic division proceeds. Simultaneously, the protein pattern was altered since in prepubertal oocytes it fluctuates, dropping and rising to levels similar to adults only at 24 hrs. In prepubertal oocytes ATP rise is delayed and did not reach levels comparable to adult ones. CLSM observations revealed that at MII, in the majority of prepubertal oocytes, the active mitochondria are homogenously distributed, while in adults they are aggregated in big clusters. Our work demonstrates that mitochondria and their functional aggregation during maturation play an active role to provide energy in terms of ATP. The oocyte ATP content determines the timing of the meiotic cycle and the acquisition of developmental competence. Taken together our data suggest that oocytes with low developmental competence have a slowed down energetic metabolism which delays later development.PLoS ONE 04/2015; 10(4):e0124911. DOI:10.1371/journal.pone.0124911 · 3.23 Impact Factor
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ABSTRACT: In this chapter, we will describe the standard technical protocols used to obtain blood vascular cast for the study with SEM, as well as we will briefly review the recent advances in the observation of vascular network through the use of this tool, and its application in the study of angiogenesis mainly according to our experience in the mammalian ovary.Handbook of Vascular Biology Techniques, 2015 edited by Mark Slevin, Garry McDowell, 04/2015: chapter Part II: pages 153-171; Springer., ISBN: 978-94-017-9715-3 (Print) 978-94-017-9716-0 (Online)
Article: Fine morphological assessment of quality of human mature oocytes after slow freezing or vitrification with a closed device: a comparative analysis[Show abstract] [Hide abstract]
ABSTRACT: Human mature oocytes are very susceptible to cryodamage. Several reports demonstrated that vitrification might preserve oocyte better than slow freezing. However, this is still controversial. Thus, larger clinical, biological and experimental trials to confirm this concept are necessary. The aim of the study was to evaluate and compare fine morphological features in human mature oocytes cryopreserved with either slow freezing or vitrification. We used 47 supernumerary human mature (metaphase II) oocytes donated by consenting patients, aged 27-32 years, enrolled in an IVF program. Thirtyfive oocytes were cryopreserved using slow freezing with 1.5 M propanediol +0.2 M sucrose concentration (20 oocytes) or a closed vitrification system (CryoTip Irvine Scientific CA) (15 oocytes). Twelve fresh oocytes were used as controls. All samples were prepared for light and transmission electron microscopy evaluation. Control, slow frozen/thawed and vitrified/warmed oocytes (CO, SFO and VO, respectively) were rounded, 90-100 mum in diameter, with normal ooplasm showing uniform distribution of organelles. Mitochondria-smooth endoplasmic reticulum (M-SER) aggregates and small mitochondria-vesicle (MV) complexes were the most numerous structures found in all CO, SFO and VO cultured for 3-4 hours. M-SER aggregates decreased, and large MV complexes increased in those SFO and VO maintained in culture for a prolonged period of time (8-9 hours). A slight to moderate vacuolization was present in the cytoplasm of SFO. Only a slight vacuolization was present in VO, whereas vacuoles were almost completely absent in CO. Amount and density of cortical granules (CG) appeared abnormally reduced in SFO and VO, irrespective of the protocol applied. Even though, both slow freezing and vitrification ensured a good overall preservation of the oocyte, we found that: 1) prolonged culture activates an intracellular membrane "recycling" that causes the abnormal transformation of the membranes of the small MV complexes and of SER into larger rounded vesicles; 2) vacuolization appears as a recurrent form of cell damage during slow freezing and, at a lesser extent, during vitrification using a closed device; 3) premature CG exocytosis was present in both SFO and VO and may cause zona pellucida hardening.Reproductive Biology and Endocrinology 11/2014; 12(1):110. DOI:10.1186/1477-7827-12-110 · 2.23 Impact Factor
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ABSTRACT: In vitro maturation (IVM) of immature oocytes retrieved from unstimulated ovaries may avoid side effects connected to hyperstimulation during IVF procedures, including the risk of cancer recurrence. In humans, the scarce availability of immature oocytes limits morphological studies. The monovular ovine may represent an experimental model for IVM studies. To assess if the scarce developmental competence of prepubertal oocytes (PO) is related to morphological changes we analyzed, by light and transmission electron microscopy, cumulus-oocyte-complexes (COCs) from lambs (30-40 days old) and sheep (4-6 years old) at sampling and after 7 h, 19 h, 24 h of IVM. Meiotic progression was determined at the same time points. At sampling, the germinal vesicle (GV) of PO was round and centrally or slightly eccentrically located, whereas in adult oocytes (AO) it was irregularly shaped and flattened against the oolemma. PO, differently from AO, showed numerous trans-zonal projections. Organelles, including cortical granules (CGs), were more abundant in AO. After 7 h, the percentage of AO that underwent GVBD-MI transition increased significantly. In PO, the oolemma was juxtaposed to the ZP; in AO, it showed several spikes in correspondence of cumulus cells (CC) endings. In PO, organelles and isolated CGs were scattered in the ooplasm. In AO, groups of CGs were also present under the oolemma. After 19 h, PO underwent GVBD-MI transition; their oolemma showed several spikes, with CC projections retracted and detached from the ZP. AO underwent MI-MII transition; their oolemma regained a round shape. CGs were located beneath the plasmalemma, arranged in multiple, continuous layers, sometime discontinuous in PO. After 24 h, both groups reached the MII-stage, characterized by a regular oolemma and by expanded CCs. PO showed CGs distributed discontinuously beneath the oolemma, while AO showed a continuous monolayer of CGs. Even if PO were able of reaching morphological maturation after 24 h of IVM, our ultrastructural analysis allowed detecting the presumptive sequence of cytoplasmic alterations connected with the delay of nuclear maturation, that might explain the reduced developmental competence of such oocytes. Data from the sheep model are of interest for zootechny, and provide an experimental basis for improving human IVM technology.Reproductive Biology and Endocrinology 11/2014; 12(1):115. DOI:10.1186/1477-7827-12-115 · 2.23 Impact Factor
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ABSTRACT: In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic organization of fresh and vitrified oocytes did not show significant changes. GVv oocytes showed significant ultrastructural alterations of the microvilli in 40% of the samples; small vacuoles and occasional large/isolated vacuoles were abnormally present in the ooplasm periphery of 50% of samples. The ultrastructure of nuclei and mitochondria-vesicle (MV) complexes, as well as the distribution and characteristics of cortical granules (CGs), were comparable with those of GVc oocytes. MIIv oocytes showed an abnormal ultrastructure of microvilli in 30% of the samples and isolated large vacuoles in 70% of the samples. MV complexes were normal, but mitochondria-smooth endoplasmic reticulum aggregates appeared to be of reduced size. CGs were normally located under the oolemma but presented abnormalities in distribution and matrix electron density. In conclusion, Cryotop vitrification preserved main oocyte characteristics in the GV and MII stages, even if peculiar ultrastructural alterations appeared in both stages. This study also showed that the GV stage appears more suitable for vitrification than the MII stage, as indicated by the good ultrastructural preservation of important structures that are present only in immature oocytes, like the nucleus and migrating CGs.Journal of Reproduction and Development 08/2014; 60(6). DOI:10.1262/jrd.2014-027 · 1.52 Impact Factor
Conference Paper: An adaptive learning agent integrated in a collaborative portal for advanced training in the biomedical field[Show abstract] [Hide abstract]
ABSTRACT: Academic communities need a collaborative portal to provide new distance learning opportunities and to improve personal training opportunities. An innovative portal must integrate an adaptive engine to assist learners individually, meeting them at their skill and knowledge levels. More than 300 biomedical students were enrolled to carry out the assessment of the new developed platform, which integrates a prototype adaptive engine based on Bayesian inference. Students who completed the web-based module left positive feedback and good overall satisfaction.FUSION 2014 - 17th International Conference on Information Fusion, IEEE, Salamanca, ES; 07/2014
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ABSTRACT: Unlike men, who have continuous spermatogenesis throughout most of their lifetime, women are born with a fixed supply of follicles, and this number progressively declines with age until the menopause. Beside age, the speed of follicle depletion can be regulated by genetic, hormonal and environmental influences. In the course of their lives, women are exposed to multiple chemicals and radiation sources that can increase the chance of developing permanent infertility and premature ovarian failure (POF). A wealth of experimental data indicate that iatrogenic (chemotherapy, radiotherapy) and xenobiotics agents (e.g., chemicals, pharmaceuticals) are potent ovotoxicants capable of accelerating ovarian reserve depletion. In the present review we reported the negative effects exerted on mammalian ovary by some widely diffused environmental chemicals, as polycyclic aromatic hydrocarbons (PAHs) and dithiocarbamate mancozeb, and by 1-3 butadiene and 4-vinylcycloexene, two occupational chemicals known to be capable of inducing ovarian cancer and infertility. Furthermore, attention has been devoted to the consequences of chemo- and radiotherapy on the ovary, both known to affect reproductive lifespan. Our increasing understanding of metabolic alterations induced by these agents is fundamental to individuate new therapeutic strategies aimed to prevent ovarian dysfunction in fertile women.Current pharmaceutical design 02/2014; 20(34). DOI:10.2174/1381612820666140205145319 · 3.45 Impact Factor
Conference Paper: A collaborative distance learning portal integrating 3D virtual labs in biomedicine.[Show abstract] [Hide abstract]
ABSTRACT: In recent years, there has been a growing interest about new tech- niques for information exchange in medical context. Academic communities need a collaborative portal with a certain degree of interactivity between speak- er and audience to provide new learning opportunities. The objective of the re- search is, therefore, addressed to provide a modular and user-friendly web por- tal that allows scientific information exchange and takes into account only the centrality of four essential elements: teachers, learners, learning-process and learning-content. This collaborative system contains also 3D learning objects to simulate highly interactive virtual laboratories. The collaborative portal is de- signed to provide an acceptable teaching tool to integrate face-to-face learning sessions with thematic web-seminars.3rd International Workshop on Evidence Based and User centred Technology Enhanced Learning, Trento, Italy; 09/2013
Article: Post-ovulatory ageing of mouse oocytes affects the distribution of specific spindle-associated proteins and Akt expression levels[Show abstract] [Hide abstract]
ABSTRACT: Abstract. The aim of this study has been to determine the effects of in vivo post-ovulatory ageing (POA) on the distribution of spindle-associated proteins, histone H3/H4 post-translational modifications and v-akt murine thymoma viral oncogene homolog 1 (Akt) expression levels. To this end, oocytes were retrieved 13, 29 and 33 h after human chorionic gonadotrophin (hCG) treatment. The presence and distribution at the meiotic spindle of acetylated tubulin, 5 g-tubulin, polo kinase-1 and Ser473/Thr308 phosphorylated Akt (pAkt) as well as histone H3 and H4 acetylation and phosphorylation levels were assayed via immunofluorescence. Akt expression levels were determined via reverse transcription–polymerase chain reaction and western blotting analyses. Spindles from oocytes recovered 13 h and 29 h after hCG treatment showed similar levels of acetylated tubulin but ageing induced: (1) translocation of g-tubulin from spindle poles to microtubules, (2) absence of Thr308- and Ser473-pAkt in 76% and 30% of oocytes, respectively and (3) a 10 significant reduction in phosphorylation levels of serine 10 on histone 3. At 29 h, a significant decrease in Akt mRNA, but not in pAkt or Akt protein expression levels, was recorded. By contrast, protein content significantly decreased 33 h after hCG. We conclude that POA impairs oocyte viability and fertilisability by altering the expression levels and spindle distribution of proteins that are implicated in cell survival and chromosome segregation. Together, these events could play a role in oocyte apoptosis.Reproduction Fertility and Development 04/2013; 26(4). DOI:10.1071/RD13010 · 2.40 Impact Factor
Article: Collaborative system for web seminars, distance learning sessions and virtual labs in biomedicine.[Show abstract] [Hide abstract]
ABSTRACT: In recent years, there has been a growing interest about new techniques for information exchange in medical context. Academic communities need a collaborative portal with a certain degree of interactivity between speaker and audience to provide new learning opportunities. The objective of the test program is to provide a modular and user-friendly web portal that allows the exchange of scientific information and takes into account only the centrality of four essential elements: teachers, learners, the learning process and the learning content. This collaborative system also contains 3D learning objects to simulate highly interactive virtual laboratories. More than 300 biomedical students were enrolled to carry out the assessment of the platform. Students who completed the web-based module left positive feedback and demonstrated good overall satisfaction with the assessment. The collaborative portal revealed an acceptable teaching alternative to integrate face-to-face instruction with thematic webinars.International Journal of Technology Enhanced Learning 01/2013; 5(3):198-212. DOI:10.1504/IJTEL.2013.059491
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ABSTRACT: The use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (OHSS) and potential cancer risk. This is also true in high risk women such as those polycystic with ovary (PCO) and polycystic ovarian syndrome (PCOS). In vitro maturation (IVM) of oocytes was primarily developed to make IVF safe for women with PCO and at high risk of OHSS. The application of IVM of oocytes to assist clinical infertility treatment remains poor because of the reduced developmental competence of oocytes after IVM, despite several decades of research. Reduced meiotic maturation and fertilization rates, as well as low blastocyst production reveal short-term developmental insufficiency of oocytes when compared with in vivo-matured counterparts. In this review, the structural role of human oocytes, revealed by different technical approaches, to the success of IVM technology is highlighted.Iranian Journal of Reproductive Medicine 01/2013; 11(1):1-10. · 0.19 Impact Factor
Article: Restoration of corpus luteum angiogenesis in immature hypothyroid rdw rats after thyroxine treatment: Morphologic and molecular evidence[Show abstract] [Hide abstract]
ABSTRACT: Thyroxine (T4) plus gonadotropins might stimulate ovarian follicular angiogenesis in immature infertile hypothyroid rdw rats by upregulating mRNA expression of major angiogenic factors. Development of growing corpus luteum (CL) is strongly related to angiogenesis and to morphofunctional development of microcirculation. Our aim was to investigate if T4 is involved in CL angiogenesis and in the activation of capillary cells and angiogenic factors after ovulation in a spontaneous model of hypothyroidism, the rdw rat. Rdw rats were treated with T4 plus gonadotropins (equine chorionic gonadotropin plus human chorionic gonadotropin; eCG+hCG) or gonadotropins alone in order to evaluate the effects of T4 on early luteal angiogenesis, on microvascular cells and on expression of major growth factors which are involved in the regulation of angiogenesis. Wistar-Imamichi rats treated with gonadotropins were used as controls. The ovaries were collected 4 days after hCG administration and analyzed using morphologic and molecular approaches. Thyroxine plus gonadotropins stimulated the growth of CLs and follicles as in controls, differently from rdw rats treated only with gonadotropins, in which CLs were not found and only small follicles, often atretic, could be recognized. In T4 plus gonadotropin-treated rdw rats CLs showed increased microvasculature, numerous activated capillaries characterized by sprouting and other angiogenic figures, and associated pericytes. Quantitative analysis revealed that the number of pericytes in T4 plus gonadotropin-treated rdw rats was comparable with that found in control rats and was significantly higher than that found in gonadotropin-treated rdw rats. The mRNA expression of vascular endothelial growth factor and basic fibroblast growth factor was significantly higher in control rats and in T4 plus gonadotropin-treated rdw rats than in gonadotropin-treated rdw rats. mRNA expression of tumor necrosis factor α, transforming growth factor β, and epidermal growth factor did not show significant changes. Our data originally demonstrated that T4 promoted the growth of an active microcirculation in developing CLs of gonadotropin-primed hypothyroid rdw rats, mainly by inducing sprouting angiogenesis, pericyte recruitment, and upregulation of mRNA expression of vascular endothelial growth factor and basic fibroblast growth factor. In conclusion, we suggest that T4 plays a key role in restoring luteal angiogenesis in ovaries of immature hypothyroid rdw rats.Theriogenology 11/2012; 79(1). DOI:10.1016/j.theriogenology.2012.09.016 · 1.80 Impact Factor
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ABSTRACT: Since the introduction of human assisted reproduction, oocyte cryopreservation has been regarded as an attractive option to capitalize the reproductive potential of surplus oocytes and preserve female fertility. However, for two decades the endeavor to store oocytes has been limited by the not yet optimized methodologies, with the consequence of poor clinical outcome or of uncertain reproducibility. Vitrification has been developed as the promising technology of cryopreservation even if slow freezing remains a suitable choice. Nevertheless, the insufficiency of clinical and correlated multidisciplinary data is still stirring controversy on the impact of this technique on oocyte integrity. Morphological studies may actually provide a great insight in this debate. Phase contrast microscopy and other light microscopy techniques, including cytochemistry, provided substantial morphofunctional data on cryopreserved oocyte, but are unable to unraveling fine structural changes. The ultrastructural damage is one of the most adverse events associated with cryopreservation, as an effect of cryo-protectant toxicity, ice crystal formation and osmotic stress. Surprisingly, transmission electron microscopy has attracted only limited attention in the field of cryopreservation. In this review, the subcellular structure of human mature oocytes following vitrification is discussed at the light of most relevant ultrastructural studies.European journal of histochemistry: EJH 07/2012; 56(3):e38. DOI:10.4081/ejh.2012.e38 · 2.04 Impact Factor
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ABSTRACT: The heart is composed by a specialized muscle, whose form and function are essentials for an adequate work and shows an amount of connective tissue which support and provide insertion for this muscle, whose collagen fibers are responsible for determination of tissue feature. Our objective was to identify the structural arrangement of the heart collagen fibers in dogs. The hearts of the dogs were submitted to the process of the controlled digestion with NaOH solution and observed by scanning electron microscope. Our results showed that the collagen fibers of the endomysial wall have structural arrangement composed by an irregular network with one layer in normal dogs but in diabetic dogs the network acquires a greater amount of the fibers and layers, looking like a "rug" of fibers modifying the relationships of the stress/strain of the tissue. Ahead of the observed results we are able to conclude that exist increase in the amount and thickness of cardiac collagen fibers, beyond the increase of layers and architectural disarrangement in the endomysial wall in the diabetic dogs.Microscopy Research and Technique 11/2011; 74(11):1018-23. DOI:10.1002/jemt.20988 · 1.15 Impact Factor
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ABSTRACT: In vitro maturation of ovarian follicles, in combination with cryopreservation, might be a valuable method for preserving and/or restoring fertility in mammals with impaired reproductive function. Several culture systems capable of sustaining mammalian follicle growth in vitro have been developed and many studies exist on factors influencing the development of in vitro grown oocytes. However, a very few reports concern the ultrastructural morphology of in vitro grown follicles. The present study was designed to evaluate, by transmission and scanning electron microscopy, the ultrastructural features of isolated mouse preantral follicles cultured in vitro for 6 days in a standard medium containing fetal calf serum (FCS). The culture was supplemented or not with FSH. The follicles cultured in FCS alone, without FSH supplementation (FCS follicles), did not form the antral cavity. They displayed low differentiation (juxta-nuclear aggregates of organelles in the ooplasm, a variable amount of microvilli on the oolemma, numerous granulosa cell-oolemma contacts, signs of degeneration in granulosa cell compartment). Eighty (80)% of FSH-treated follicles formed the antral cavity (FSH antral follicles). These follicles showed various ultrastructural markers of maturity (spreading of organelles in ooplasm, abundant microvilli on the oolemma, scarce granulosa cell-oolemma contacts, granulosa cell proliferation). Areas of detachment of the innermost granulosa cell layer from the oocyte were also found, along with a diffuse granulosa cell loosening compatible with the antral formation. Theca cells showed an immature morphology for the stage reached. Twenty (20)% of FSH-treated follicles did not develop the antral cavity (FSH non-antral follicles) and displayed morphological differentiation features intermediate between those shown by FCS and FSH antral follicles (spreading of organelles in the ooplasm, variable amount of microvilli, scattered granulosa cell-oolemma contacts, signs of degeneration in granulosa cell compartment). It is concluded that FSH supports the in vitro growth of follicles, but the presence of a diffuse structural granulosa cell-oocyte uncoupling and the absence of theca development unveil the incomplete efficiency of the system. The present study contributes to explain, from a morphological point of view, the effects of culture conditions on the development of mouse in vitro grown follicles and to highlight the necessity of maintaining efficient intercellular communications to obtain large numbers of fully-grown mature germ cells.Reproductive Biology and Endocrinology 01/2011; 9(1):3. DOI:10.1186/1477-7827-9-3 · 2.23 Impact Factor
- Reproductive biomedicine online 10/2010; 20. DOI:10.1016/S1472-6483(10)62515-1 · 3.02 Impact Factor
Article: Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols[Show abstract] [Hide abstract]
ABSTRACT: To ascertain possible cell damage from cryopreservation, the ultrastructure of human oocytes cryopreserved by slow cooling was assessed. Cryopreservation was performed through two protocols with one-step or two-step propanediol. Fresh control oocytes were examined for comparison. Samples were processed for transmission electron microscopy analysis. By light microscopy, both fresh and frozen-thawed oocytes appeared regularly rounded, with intact zona pellucida, and homogeneous cytoplasm. By electron microscopy observation, organelles were abundant and uniformly dispersed. Mitochondria-smooth endoplasmic reticulum associations appeared regular. However, both the amount and density of cortical granules appeared abnormally reduced in frozen-thawed samples. Slight to moderate vacuolization was also found in the ooplasm of oocytes of both frozen groups. Slow cooling ensures a good overall preservation of human oocytes. However, cytoplasmic vacuolization and cortical granule loss appears associated with cryopreservation, irrespective of the protocol used.Journal of Assisted Reproduction and Genetics 02/2010; 27(4):131-40. DOI:10.1007/s10815-010-9394-7 · 1.72 Impact Factor
Article: Morphological expression of angiogenesis in the mammalian ovary as seen by SEM of corrosion casts[Show abstract] [Hide abstract]
ABSTRACT: In the mammalian ovary, follicular and corpus luteum cycle is associated with intensive microvascular remodelling. The complex angiogenic dynamics are finely tuned by numerous regulatory factors acting as activators (up-regulators) or inhibitors (down-regulators) of angiogenesis. Alterations of such a tight modulation are involved in several pathologies, including infertility, polycystic ovarian syndrome, ovarian hyperstimulation syndrome and ovarian cancer. We have demonstrated in several experimental models that ovarian function is critically and specifically dependent on angiogenesis for follicular development, ovulation, and corpus luteum growth. The aim of this review is to summarize the results we have obtained on the morphodynamic remodelling of ovarian microvascularization, in polyovulatory (rat, rabbit and pig) and monovulatory species (cow), using scanning electron microscopy of vascular corrosion casts. The knowledge of the morphological expression of the up- and down-regulation of angiogenesis occurring in mono and polyovulatory animals might provide useful information to preserve fertility and to increase of the effectiveness of reproductive management in species of domestic interest.Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia 01/2010; 115(1-2):109-14.
Top Journals
Università degli Studi dell'Aquila
Aquila, Abruzzo, Italy
- • Department of Life, Health and Environmental Sciences
- • Center for Microscopy
- • Department of Experimental Medicine
Tohoku University
Sendai-shi, Miyagi-ken, Japan
- Graduate School of Agricultural Science
Catholic University of LouvainЛувен-ла-Нев, Walloon, Belgium
Sapienza University of Rome
Roma, Latium, Italy
- Department of Internal and Specialized Medicine
Toho University
Edo, Tōkyō, Japan
- Department of Obstetrics and Gynecology
Okayama UniversityOkayama, Okayama, Japan
Università degli Studi di Messina
Messina, Sicily, Italy
- Dipartimento di Medicina Clinica e Sperimentale


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300+ Apartment Rentals · Kotor
300+ Apartment Rentals · Kotor
Newly restored studio, in the backyard of the old stone villa. Waterfront location, just 10 minutes easy walk away from the Old Town. Two small pebble beaches are just a minute away. Ideal for couples. Learn More
Peaceful Location On The Waterfront, Just 10 Minutes Away From The Old Town Of Kotor (shops, market, restaurants, bars, concerts, cultural events, museums, churches). Private parking, and small pebble beach in front of the house. Grocery shops - 3 minutes walk away. Perfect for couples.
Located in the UNESCO-protected Old Town of Kotor. Set in a traditional stone house on the ''Piazza of salad'' (Pjaca od salate), on the Main entrance to the San Djovanni fortress, only a few step from the St. Tryphon Cathedral. Learn More
Studio apartment offers private entrance, terrace, free Wi-Fi, air-conditioned with LCD cable TV. Consist two beds first one with large double bad, and second sofa bed for sleeping two more person. Kitchen (fully equipped) with dining area. Equipped with washing machine, iron, iron table, hair dryer, etc, so the guests no need to carry nothing except their personal goods. Studio can accommodate up to 4 persons. Positioned in the heart of Old Town surrounded with many restaurants and ...
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In a quiet backstreet of Kotor Old Town, Montenegro's quaint and charming pedestrian-only walled village, with many shops and restaurants. Newly renovated, English owned, two bed apartment with en suite bathrooms and large kitchen/sitting room. Learn More
On the ground and first floors of an 18th century old stone building, comfortable and spacious, with airconditioning for the summer and heating in the winter. Your own home from home in Montenegro... You will have the whole two bedroom, two bathroom apartment to yourself. Downstairs toilet and tiny courtyard outside... There is keybox access, so if your flight is delayed or you are late there is no problem about getting into the apartment. I am always on email contact and my assistant ...
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Apartment Kotor Bay is located within the UNESCO-protected historic centre of Kotor. This One Bedroom Apartment offers air-conditioned accommodation with free Wi-Fi access. It is just 400 metres away from the public beach. Learn More
This newly renovated One Bedroom Apartment is located in historic building dated back to 13th century. All rooms fitted with wooden furniture and with traditional stone walls and wooden beams. The size is around 50 square meters. Two single beds are located in the living room and one king size bed located in the bedroom, so can comfortably sleep 4 people, and perfectly fits families or company of friends.There is also small shared terrace available for all guests. Cathedral St. Tryphon, ...
apartment in authentic haus in old town of Kotor, with wonderful terase and the wiew of the see and the mountains over the old town roofs. the place is isoleted, preety quiet, and you can heer the birds singing uring the Sun bath... Learn More
very old haus in the old town, near to the old castle wall. nice 3 pieces apartment, and a wonderful terase with wiew over the old town roofs, mauntains and see. all of them yes public transportation is wery close. like the public free or pay parking.
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Apartment in Central Location 50 sq/m 4 stars 200 meters from Waterfront Sleeps 2/4 (1 Bedroom + Sofa bed) Learn More
The apartment is situated in the heart of the ancient magical walled town of Kotor --a World Heritage Site, UNESCO-protected. The Old Town has a great number of monuments of medieval architecture, churches, cathedrals, palaces and museums. The apartment is in a very good quite location, with shared weave. Restaurants, shops (including a supermarket), café bars, and the 17th century Grgurina Palace (which today houses the Maritime Museum) are all just seconds away. The apartment is less th...
Apartment Old Town Boskovic is the right place if you'd like to discover our fantastic city. It is situated in the lively small square: a few steps from St. Tripun cathedral. The building is positioned in the oldest street in Kotor- 13th century . The Apartment is a nice and completely furnished private apartment, located in the lively Old town, in the heart of the Kotor. The Apartment has an independent front door and is composed by a kitchen, completely furnished with fridge, and sitting ...
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The apartment are newly built luxury,total area is 48m2 and has a living room,kitchen, bathroom, one bedroom and in living room soffa bed for two.The apartment have free HiFi wireless internet and cable TV, and free parking space for our quest. Learn More
Apartment here comprise a seating area with a sofa and cable TV. There is a fully equipped kitchen or a kitchenette with a dining area. The private bathroom come with a shower and a hairdryer Learn More
Located in a quiet part of Kotor, 300 metres from the Old Town, Studio Apartment Sandra offer a spacious garden furnished with table and chairs and parasols. Free Wi-Fi is provided, as well as free parking on site. Apartment here comprise a seating area with a sofa and cable TV. There is a fully equipped kitchenette with a dining area. The private bathroom come with a shower and a hairdryer. I am always ready to respond on any question or request so please do not hesitate to ask whatever ...
Studio near Old Town Kotor - 40 m from the sea. Studio is situated on the ground floor in a family house in Muo. The house is isolated from the car traffic. Learn More
Newly build studio with full privacy which you make fill comfortable. Studio of 25 m2 consists of a bedroom/living room, kitchen (fully equipped) and bathroom with shower. The studio can host up to 2 guests. Studio has terrace which is ideal for a peaceful rest surrounded by figs, oranges, pomegranates, olives trees … and greenery. NOTICE: NOT available. Please do...
Located in the Old town of Kotor, close to the beach and close to the main bus station, this bright and cozy fully furnished apartment is perfect starting point to explore the city. Learn More
Recently completely renovated and equipped with new furniture and appliance, apartment is brand new. Although studio, it has 32 square meters ( 344 sq foot) and can comfortably accommodate up to four. It has air conditioning, LCD TV, cable TC, wireless internet. Bathroom has a shower cabin and kitchen is fully equipped. Guest have access to the whole apartment and everything in it. There are no shared areas. I'm there to answer your questions and help if I can, but I won't be bothering yo...
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Set on hill with amazing view on Kotor Bay and Historical ramparts. Positioned directly under the mountain Lovcen, refreshing air mixture of mountain and sea breeze provides unique experience of beautiful morning to all guests of Studio Jelena! Learn More
Master's Apartment - 50m2 Superior Studio "Jelena" is set on nicest hill in Dobrota as a part of condominium "Silver Moon" with amazing view on Kotor Bay and Historical ramparts. Positioned directly under the mountain Lovcen,refreshing air mixture of mountain and sea breeze,provides guests with unique experience of first morning coffee in calm residance of Master's Apartment surrounded by centuries old olives trees. Free private parking is available on site outdoor and in garage. There is ...
A bright and airy double bedded room, ideally located in a quiet street inside the medieval town of Kotor. Kotor offers many cafe bars, restuarants, historical monuments and buildings. Kotor is an ideal location from which to explore montenegro Learn More
The Studio is located on the third floor which being an old building is only accessed by stairs !Tourist tax is not included in the price which is 1e per person per night!
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The apartment is located on the boardwalk in Dobrota, at a distance of 30 meters from the sea and 500 meters from the old town of Kotor. Nearby are: supermarket, drug store, "Health" and numerous bars and restaurants. Learn More
The apartment is 40 square meters hallway, bathroom, kitchen, bedroom and balcony. Air-conditioned, TV, cable, internet. Kotor is located at: - a half-hour drive to the luxury port "Porto Montenegro" in Tivat , - a half-hour drive to Budva, - 1 hour drive to Cetinje, - 1.5 hour drive from the Mausoleum on Mount Lovcen, - 2-hour drive to Dubrovnik, Trebinje and Podgorica. - Airport Tivat is a half hour drive. - Podgorica Airport is a 2-hour drive. - Airport Cilipi in Croatia ...
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The apartment is 65m2. It consists of two bedrooms, one double bed and two single beds which can be connected, as well as the sofa that can serve as an extra bed for two people. Fully equipped kitchen (dishwasher, stove, oven, refrigerator). Learn More
The space The apartment is 65m2. It consists of two bedrooms, one double bed and two single beds which can be connected, as well as the sofa that can serve as an extra bed for two people. Fully equipped kitchen (dishwasher, stove, oven, refrigerator). The bathroom has a shower and washing machine. Guests can use a shared terrace behind the house. It is important to know that in the vicinity of the house cca. 2 mns are: supermarket, bakery, post office, pharmacy, local bus stop, ATM. - The ...
Studio apartment 3m from the sea. The brand new Studio apartment is situated in a NEW house 3 m from the sea in a little quite place Muo near Kotor (1.5km) . It is 10 minutes by walk from the picturesque Old town of Kotor. Learn More
Studio Apartment of 40 m2 consists half separated space , bath and sea view living room with kitchen and terrace . The apartment can host up to 4 ( double bed for 2 persons , living room flip bed for 2 persons) and perfectly fits both for families or company of friends. The house has a terraces and small garden, which is the perfect place for having rest under the shady palm tree. The apartment has: - everything for cooking at home; - fridge; - parking place. Garden is used as a...
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Set in a Mediterranean garden with palms, Tianis offers serviced apartments 50 meters from Kotor's Old Town and 50 meters from the beach. It features accommodation with private balconies and free Wi-Fi. Learn More
... Parking, wifi etc ..
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Apartment with a view of old fortres and old Town of Kotor.the old town is 10 minutes walking from the apartment and the road is near the sea.The apartment is located at the botom of hill Vrmac and is also near the sea. Learn More
In apartment there are two beds and one opening bad.There is also a small kitchen and inside bathroom with a shower.Beautiful view from apartment on fortress ,old town Kotor,port,marina and bay.The parking is behind apartment and its free of charge. The guests have access to a terrace with outside kitchen and grill,wifi free,outside shower.In apartment the guests have access to everything inside of the apartment. Normaly everything depends of the guest wishes.I can organise rent a car,and...
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Buy houses in Ladybarn Road, Fallowfield, Manchester M14 - 5 bedrooms
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Richmond Road, Fallowfield M14Terraced house for sale - 5 bedrooms
Flax & Co. Is delighted to offer to market this superb 5 bedroom investment property in the heart of Fallowfield's student community. Each of the bedrooms is able to comfortably fit a double bed in. The property is expected to bring in a rental ... More details
Note: Distances are straight line measurements
- Mauldeth Road (0.4 miles)
- Levenshulme (0.9 miles)
Marketed by Flax & Co, M20. Call 0161 937 6362


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Hourly - Intermediate ($$) - Est. Time: Less than 1 week, 30+ hrs/week - Posted
We are looking for a designer to create a creative illustration and some icons for our landing page. We want something that depicts a before and after contrast to a persons life after we help them with their business. Here are some sample images: http://madebyshape.co.uk/ http://www.relayme.be/ We are looking for a really creative graphic artist as we have other work related to this project. For consideration please submit your best examples
- Number of freelancers needed: 2


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"… and that's when my daughter, apropos of nothing (wider, please), announces she's going out trash-can tipping with some boy from college (hold still, thanks) I've never even met! Well, I tore into her… say, you've stopped flossing, haven't you?"
"… and that's when my daughter, apropos of nothing (wider, please), announces she's going out trash-can tipping with some boy from college (hold still, thanks) I've never even met! Well, I tore into her… say, you've stopped flossing, haven't you?"
Here's the deal, People. If you check thees McLink, you will notice that Cute Overload and Dilbert are neck and neck for top calendar in Amazon's Humor & Comic Calendars section. We must step eet up! Dilbert's calendar is all about living in a drab, fabric-paneled cube. Fair enough. But ours is all about BRINGIN' DA QTE and brightening up that drab, fabric-paneled cube.
So buy the 2013 Cute Overload Calendar now, in the Desktop version for Desktop People, and the Wall Calendar version for…Wall People. And make sure you tweet this link to all your Peeps! Everyone that tweets this link will receive…..our undying appreciation! Think of eet! Operators are standing by, maybe even Mr. Bezos! (Prolly not, though.)
Love ya Scott. Really.
P.S. First person who 'shops Bezos holding up two calendars in his hands WINS TWO CALENDARS!
Will you please check out Little Dude/Dudette, a resident of the Monterey Bay Aquarium. The Sea Otter exhibit is closed for the moment, so we can't bring you a live look at the little guys- but here's a live shot of Monterey Bay itself, and maybe a wild sea otter will swim into view.
From the Monterey Bay Aquarium Facebookster and Tumblr page.
Separate but equal never works, Robotix.
I see we got some darn fine knobullarity here, wouldn'tcha say?
So, this is Molly when she was a bebeh. Mama didn't let Molly nurse, so those nice Tufts Veterinary Medicine peeps down there fixed 'er right up. Ya sure, the neck bandage was for her catheter, don'tcha know. Well, Molly's three and a half years tall now and she's doin' just okie dokie!
I really love it when no one else is home so I can take off my socks, leave 'em where they lay and then hog up the whole couch.
Just try to relax, Pacotaco724.
Don't know what it is about 'em, but these chicks are just drawn to me, man. Some cats just got it and others don't, I suppose.
Via YouTuber IGNORAMUSKY.
Every day when I walk to school, The Cat stands in the middle of the sidewalk and won't let me pass unless I give him my lunch money. If I don't, he says he's gonna "Pound me one!"
This is all so embarrassing.
Via Attack O' Da Coot.
People, this is Monty. You might not know the name, but you'll remember his prosh face from earlier this summer.
Monty has died at the age of 13. He was one of Queen Elizabeth's three Corgis that were shown in the James Bond-themed Olympics opening film clip. Two other Pembroke Corgis remain in the Palace, Willow and Holly.
Here's more on the Queen and Her Majesty's Puppehs.
Full deets here.
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The HandleBand
I wasn't completely sold on the idea of this mount but my rockstar girlfriend has debated with me over coffee that this design is very thought out and will benefit many people. Not so secretly, they had me at bottle opener.
What Is The Handleband?
Our phones help us navigate and train. They're our cameras, our lights, and our emergency link home. So why don't we use them with our bikes?
The Handleband is a simple tool that brings to light the untapped potential of our smartphones. It offers a simple, elegant and universal means of uniting your smartphone with your bicycle.
Here are a few things that make it unique:
- (1) In use, it is smaller than a deck of cards.
- (2) It is removable (so its doesn't clutter your ride)
- (3) It works with any phone, bike and case.
- (4) It is simple and robust. (One reinforced unit)
- (5) It holds a lot (flashlights, pumps, diplomatic flags)
- (6) It is a bottle opener.
Check out Handleband.com and Kickstarter page
12 Comments
I listen to 1950s R&R on Pandora when I ride and it's a PIA to reach into my pocket for my phone to give a thumbs up or down on a song. This device sounds like an great solution to my problem.


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Select Local Merchants
John's Old Time Barber ShopRead MoreA classic barber pole is the first harbinger of Scott Anthony's Classic Barbershop's commitment to old-fashioned clipping techniques. Inside, clients lounge in large leather chairs in front of full-length mirrors and await an in-depth consultation to determine ideal hair length and rock star potential. Master barber and educator Scott Braga trims coifs into close-cropped cuts and fades, while Stephen Guillian renders jaw lines silky-smooth with soothing hot-towel shaves. The team wields their steel tools to create looks of any degree of difficulty, from shaved-in hair designs to distinguished whisker trims.194 West StreetMilford, MA US
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Diorio'sRead MoreNothing will make you feel more uplifted than a relaxing and professional hair care service from Diorio's in Worcester. Enhance your skin and get your glow back with a facial from this salon. Maintain your skin's health with a professional skin treatment, such as facial peels. Increase mobility and improve posture with a full body massage from this salon. Dealing with unwanted body hair? Diorio's understands and offers many services, including waxing and laser hair removal. This salon offers the perfect mani and pedi combination to keep your nails in style. Looking for make up application? Head to Diorio's for all of your beauty and cosmetic needs. From hair cuts and color and highlights to custom cuts and colors, the stylists at this salon are here to assist with all of your hair wants and needs. Yes, what you have heard is true! This salon accepts walk-ins for all beauty services. Diorio's prides itself on accepting various major credit cards for convenient payment. If you're driving, be sure to take advantage of the nearby lot. For true style and finesse, be sure to put your head in the hands of Diorio's.335 Plantation StWorcester, MA US
Mocine Salon and SpaRead MoreCraving a summer glow? Check out the tanning services at Mocine Salon and Spa in Worcester. A professional facial from this salon can reveal younger, healthier looking skin. To instantly hydrate and moisturize your skin, treat yourself to a skin care treatment from Mocine Salon and Spa. This salon offers a wide range of hair care services, including up-dos and color processes. Get rid of your stress with a relaxing massage from this salon. This salon offers professional nail enhancements that are both fashion-forward and seasonally trendy. Making an appointment is the only way to get through the doors of this highly-rated establishment. Paying by major credit card is easy and convenient for customers of Mocine Salon and Spa. Mocine Salon and Spa's guests can take advantage of the easy street and lot parking options. So ditch that at-home formulas and try a new kind of tan at Mocine Salon and Spa.258 Park AvenueWorcester, MA US
Saigon Nail SupplyRead MoreSaigon Nail Supply in Worcester is a craft nail salon that offers trendy and fashion-forward nail designs. Saigon Nail Supply will keep you looking your beauteous best with such services as make up application. Help your nails become more beautiful, stronger, and healthier with the terrific nail care treatments at this salon. Whether you want a fresh clean coat or cool nail art, the professionals at Saigon Nail Supply can give your nails the desired look.75 Green StWorcester, MA US


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Re: [tied] Whence Grimm?
Expand Messages
- --- In firstname.lastname@example.com, george knysh <gknysh@y...> wrote:
> --- tgpedersen <tgpedersen@h...> wrote:Correction: From Thuringia to Jutland and Fyn. Not Sjælland. Not the
> >
> > So what I would like to know is: in what way does
> > "the prevailing
> > view" construe a path of continuity from the
> > Przeworsk culture to the
> > later Germanic speaking ones
> I don't think it can
> > be denied that
> > Przeworsk was _a_ root of Germanic culture, but it
> > seems those roots
> > were widely divergent.
> *****GK: The argument seems to run thus (there are
> many sources for it; I have relied on the more recent
> Polish, Russian, and Ukrainian literature):
> The source of Germanicism in the east is the area of
> the Jastorf culture which existed in North Germany and
> Southern Scandinavia from the mid-first millennium BC.
Scandinavian peninsula.
> Many Jastorf groups migrated eastward beginning ca.Evidence? Not that I'm absolutely opposed to that; some of Kuhn's
> 300 BC, sometimes in conjunction with La Tene groups
> (Celtic) sometimes independently. They mixed with
> "local culture" groups there, and after a period of
> co-existence contributed to the emergence of new
> cultures most of which in the progress of time became
> preponderantly Germanic as to language.
supposed Nordwestblock words appear in North Germanic, which is
difficult to understand if they belong in the Nordwestblock
geographic area, since the invasion into Scandinavia by Germanic
speakers shouldn't have brought these words with them then.
>Przeworsk wasThat matches with the fact that the northern province of Jutland,
> one such culture. The early complex mix involved Celts
> (La Tene) Germanics (Jastorf) and "locals" (Late
> LUsatian, Pomorian etc.)of uncertain IE speech. By the
> period of the Roman Empire, Przeworsk can be
> associated with the historical Vandals.
north of the Limfjord which has evidence of Oder-Warthe intrusion is
named Vendsyssel < Wendlæ syssæl, the inhabitants are called
>The claim isAccording to Peschel and Jan Derk Boosen: "Das Oder-Warthe-gebiet in
> not that P. is THE source of Germanic culture. It is A
> Germanic culture. So when P. backflows into Jastorf
> (as you say) it is as though one Germanic culture
> mixed with another.
der Przeworsk-Kultur während der ausgehenden vorrömischen Eisenzeit
und der älteren Kaiserzeit" the Oder-Warthe elements in Jastorf
appear foreign. But we are quibbling over words. You are stressing
the Jastorf roots of the Przeworsk culture, I the eastern ones.
Besides, with the merging of two "Germanic" cultures we would expect
a number of words in Germanic that appeared similar, but were not
identical. That is not the case, apart from some of the supposed
Nordwestblock words.
>It is believed that the earliestNow that was a bad tactical move. Once you mention the Skiri, you'll
> attested name of a Germanic group in the east is that
> of the SCIRI (Skiroi of the Olbian inscriptions). This
> name was earlier analyzed on Cybalist. The date for it
> is "sometime prior to 230 BC".
bring up the Bastarnae, who, according to the contemporary sources,
were Skiri mixed with Sarmatians. This is of course not an
archaeological argument; don't hesitate to indicate if you feel it is
BTW the names 'Sciri' and 'Bastarnae' are good evidence that these
peoples spoke some type of Germanic. And the word 'saddle' (PGmc
*saðula, cf Slavic sedlo, Latin sella < *sed-la) which because it is
different from what we'd expect in an inherited Germanic word (cf.
Geraman Sattel "saddle", Sessel "seat") must be a loan. The /a/
for /e/ indicates Indo-Iranian, the retained /s-/ instead of
Iranian /h-/ indicates Indian (of which some languages still remained
at the Sea of Azov), and although Classical Sanskrit has /r/ for /l/,
Eastern Prakrits show that some Indic dialects might have had /l/.
The Oder-Warthe intrusive aristocratic graves in Jastorf occasionally
have spurs, so they must have been a mounted warrior caste.
> Ivo Hajnal, Methodische Vorbereitungen zu einer Paleolinguistik deslit.
> Balkanraums, in
> Bammesberger, Vennemann(eds.), Laguages in Prehistoric Europe
> ...vgl. thrak. (gloss.) skálme: "Schwert" < */skolma:/ (vgl.
> skélti)...Mangelhafte Erklärung seitens von Ivo Hajnal da es keine
> Torsten
ernstzunehmende Quelle bennent:-)
I suspect he is not the only one. It happens I have fount recently
the same mention in an another work and this is "Ancient Languages of
the Balkan" of Radoslav Katic^ic^
He mention too the word "skalme" but as Ivo Hajnal, there is no
reference where is mentioned this glosse and why it should
mean "sword". Again the same odd style of " we suppose it meant
so_and_so" because "see Lit. skelti" :-))
Thank you Thorsten . It appears there are a lot of shcolars which
simply copy each other without bother to verify what about. So bad
for a lot of conclusions from a such manner to abord the things.
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Sponsored By
Belleville Man Charged With Animal CrueltySparky the dog was found with muzzle taped shut.
Former Pro Randy Wells Opens Belleville Baseball CenterFormer professional baseball player Randy Wells has opened a baseball academy in southern Illinois and is coaching varsity baseball at a Catholic high school in Belleville.
Belleville Hospital Significantly Reduces Blood UsageHSHS St. Elizabeth's Hospital in Belleville is honored after it reduces the amount of blood used by 30 percent.
Officials Begin Process of Dissolving Belleville TownshipCurrently, Belleville residents have a line on their property tax bill for Bellville Township, which has the same borders as Belleville. The owner of a $100,000 home in the city pays about $40 each year to fund the township's budget.
Wanted Belleville Man Surrenders to St. Louis PoliceSt. Louis police say a Belleville, Ill., man wanted for a fatal New Year's Eve accident is now in custody.
Man Charged in Swansea Stabbing That Left Woman DeadAlvin Harris has been charged with first-degree murder in connection with a stabbing at a southwest Illinois apartment complex that left a woman dead and her 9-year-old son severely injured.
Motorcycle Accidents Claim Three Lives2 killed in Pike County, 1 in St. Louis County
Non-Stop Flights to Vegas Out of Mid-America Airport to Begin in NovemberAllegiant Air manager of airports Eric Fletcher says it's already a hot ticket, with an introductory fair - all taxes and fees included - of $49 each way.
Belleville Man Killed in Motorcycle CrashSt. Clair coroner's office identifies victim as Jackie Reichling
Man Charged in Sexual Assault of Mehlville GirlA man has been charged with the sexual assault of a 11-year-old girl he allegedly followed home from a grocery store last Friday.
Belleville Police May Still Wear American FlagsChief Bill Clay rescinds uniform policy outlined July 7th
CEO of Belleville Hospital Announces ResignationThe CEO of St. Elizabeth's Hospital announced Monday that she will leave the hospital at the end of July.


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Politicians take credibility
Published: Sunday, October 24, 2010 at 3:30 a.m.
Last Modified: Monday, October 25, 2010 at 11:18 a.m.
Dear Editor: It's time the people of this state wake up. Realize politicians need to take credibility for their own actions and wrong doings. We as voters need to open our eyes to whom really runs the political ring; the machines of Alabama are the big businesses and special interest groups.
Definitely a lot of handshaking going on, I'll do for you if you do for me tactics, a bunch of glorified political favors.
As for (Robert) Bentley, he is another Guy Hunt, a puppet for the Republican Party. Another Jed Clampett, no clue about what's going on but controlled by the greedy "Drysdales" of this state. Who would do anything to cater to their whims?
I think voters need to ask themselves, are the politicians in the political positions for the populace and the good of this state or themselves and their special interest groups?
No business good or bad is being allowed to come to the floor and if we're stupid enough to send more Republicans to office, this will only get worse, much worse.
The hard working class will be the ones to suffer for all the corruption taking place in our political platform. We need leaders to hear and promote the peoples' interest and not turn a deaf ear to the citizens of Alabama. Go to the polls Nov. 2 and allow your voice to be heard!
Elizabeth Alexander
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