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The Tonics® Ladies' Cosmic AST Back-Zip Paddock Boot in European leather has a convenient rear zipper and an ergonomically-shaped pull for easy on/off. This paddock boot is built for durability.
Technical detailing in the Tonics® Sirius AST Half Chap maximizes fit. This half chap in European grain leather has an ergonomically-shaped elastic insert. A durable, elasticized strap allows it to be attached to the boot. Durable, anti-slip leather on the inner calf ensures grip.
The Tonics® Ladies' Space UST Zip Paddock Boot in top-quality, soft Italian leather is waterproof and breathable. This ladies' paddock boot closes with a double-sided, waterproof YKK® front zipper with an easy-to-hold pull. Elastic inserts and a padded tongue area provide a comfortable fit. Press-button system attaches this boot to the Galactic UST Half Chap.
The Tonics® Ladies' Stardust AST Paddock Boot is made of European leather with elasticized laces for enhanced comfort. The patented X-static® orange mesh lining with silver threads keeps foot dry.
Anatomically-shaped details enhance the fit and grip of the Tonics® Triton AST Half Chap. Made of European leather, this half chap features an ergonomically-shaped rear leather flap with elastic insert for optimal calf adjustment. Durable, anti-slip leather on the inner calf ensures grip.
Slip your feet into the soft, warm comfort of these Smartwool® Traditional Snowflake Socks. Made of an itch-free, mid-weight merino wool blend, these soft, wool winter socks regulate your foot temperature, wick moisture and resist odor.
Tredstep™ Ladies' Giotto Front-Zip Paddock Boot offers European-inspired styling and the convenient on/off of a front zipper. Made of supple full-grain nappa leather, this paddock boot is loaded with comfort features.
The Tredstep® Side Zip Half Chap is made with super-soft, full grain leather and lined with soft amara suede. This half chap is designed to offer a personalized fit and a comfortable ride from day one. It has an elegant Spanish topline and subtle stretch panel over the forefoot and front. Full-length side zipper closes from top to bottom.
Tredstep Custom Fit Half Chaps give you the feel of a high-performance, custom-made half chap. In premium soft, oiled full-grain leather with a full leather lining, this elegant half chap molds to your leg for security and fit. A stretch panel along the back zipper eases the fit and allows for the expansion of leg muscles while riding.
The Tredstep™ Deluxe Half Chap in full-grain leather offers a great fit and comfort thanks to its stretch leather panel. Heavy-duty outside YKK® zipper with keepers at top and bottom.
The Tredstep™ Donatello Field Boot provides comfort and an exceptional fit that supports top performance. In high-quality, full-grain napa leather with a supple leather lining, this slim-fit riding boot follows the contours of your leg with its rear stretch panel and top gusset. Tall height.
Tredstep Half Chaps-the original in stretch suede from Tredstep Ireland™-mold snugly to your legs for optimal performance and comfort. An elastic back panel offers the right amount of give for a precise fit. The full-length side zipper closes from the top down. A great value in a comfortable chap!
The Tredstep Ireland™ Pure AirCool Socks offer moisture wicking and cushioning without bulk. Has added piqué weave panel in the upper foot and foreleg for cooling ventilation. Dryarn in the foot. Fits comfortably inside boots.
The Tredstep™ Ladies' Michelangelo Dress Boot blends form and function for a high-performance, incredibly beautiful boot. It's handcrafted in Italy from exquisite materials and with painstaking attention to detail. The carefully shaped calf ensures a sculpted, personalized fit. Thermo Molded Insoles deliver comfort, stability and support.
The Tredstep™ Ladies' Michelangelo Field Boot will please even the most discerning riders. Handcrafted and beautifully designed, this high-performance field boot features exquisite hand-sourced materials. The carefully shaped calf offers a sculpted, personalized fit. Thermo Molded Insoles provide comfort, stability and support.
Tredstep™ Raphael Boots define a new category of tall boots with a refined design and enhanced performance that flatters you no matter your discipline. These luxurious boots are fashioned of exquisite Italian analine calf leather with a full grain leather lining and a high performance outsole. An elegant profile and super-close fit is achieved by the discreetly covered rear stretch panel on the inside and top gusset.
Serious riders will appreciate the high-tech performance benefits of the Tredstep Ireland™ Solo Pro Sock. Made of advanced technology yarns, this riding sock protects the heel area with anti-friction Tencel®. It has a cushioned foot with odor-neutralizing fibers that keep the foot fresh all day. Moisture-wicking properties ensure that skin stays dry.
The Tredstep Ireland™ Solo Sport Sock is ideal for the active rider. This moisture-wicking, cushioned, riding sock keeps skin dry as odor-neutralizing Deocell® fibers neutralize odor and wick moisture. Great inside riding boots, barn boots and everyday footwear!
The Child's TuffRider® Micro Touch Half Chap is designed with the look of a tall boot in mind. Made of synthetic stretch leather, this easy-care half chap for children has a discreet rear zipper that closes from top to bottom. It's styled with a high-cut topline, sewn-down swagger tab and zipper keeper.
The TuffRider® Starter Paddock Boot pleases beginners--and anyone--with its easy-care, super-soft faux leather. This synthetic, all-weather paddock boot with zipper carries you through any weather conditions.


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Article ID: 2832072 - View products that this article applies to.
After you boot your computer to the Windows 8 or Windows 8.1 Start screen, you may find that some or all of the Windows 8 and 8.1 app tiles have an "x" in the lower-right corner. When you click one of the app tiles to start the app, you receive the following message:
This app can't open
Check the Windows Store for more info about <AppName>.
Go to the Store
This behavior may be caused by an error that occurred when an app package was first installed or injected into the Windows 8 or Windows 8.1 image. Or, it may be caused by out-of-date software.
This issue may be resolved by just waiting several minutes, as the issue may be fixed by Windows.
If the issue is not resolved after several minutes, first try checking for Windows updates, as updating Windows may fix the problem. To check for updates, follow these steps:
If updating Windows does not work, you may be able to resolve this issue by either installing a new app from the Windows Store or by reinstalling or updating existing apps. Doing this may update or fix a dependency that multiple apps rely on.
If this does not resolve the issue, you may have to contact the original equipment manufacturer (OEM) that you purchased the computer from. Or, contact Microsoft Support.
More information
If you are the individual or engineer who creates the Windows image, you may want to closely monitor the injection of app packages to look for errors or messages that indicate that there was a failure. KB article 2813187
(http://support.microsoft.com/kb/2813187)provides some of the logging information that's used by the Windows Store Inbox Applications Diagnostics tool for troubleshooting Windows 8 apps. This information may be useful in your investigation.
This issue may also occur after you perform a refresh of the system by using the push-button reset features. For more information, see Push-Button Reset overview
Article ID: 2832072 - Last Review: January 21, 2015 - Revision: 5.0
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Context of questionable relevance It was exactly one year ago today that I first uttered the phrase, "paleo is...
Archive for category: Science
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It should be no secret that I'm no fan of regurgitated arguments. If you're going to recite a standardized...
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Preface *Skip to below the videos if you don't care about an aside about doctors. I almost feel bad...
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Prepare yourself to be (not at all) surprised: Alexander McQueen has made something cool that I desperately want. Now prepare yourself to be (not at all) surprised again: it involves skulls. This is a label that doesn't even need to surprise you to turn out something edgy and effortlessly cool…McQueen is that good. The item in question this time is the Cameo Silk Scarf, which features a framed skull portrait print and a McQueen logo in the corner (because let's face it…if you're wearing McQueen, you want everyone to know). (Price: $395.00 BUY)
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October 03, 2015
Models, not celebrity guests, turn heads in Paris
Forget the celebrity guests; it's all about the models at this season's Paris Fashion Week. Kendall Jenner and Gigi Hadid ...
October 02, 2015
Cindy Crawford talks selfies, models today and daughter Kaia
Score one for the selfie stick: Cindy Crawford believes those photos of you by you can help boost self-image.
October 01, 2015
Kendall Jenner steals Balmain as Rihanna arrives in Paris
Celebrity is in the air at Paris Fashion Week as Rihanna — in a baggy black hoody, shades and sandals — arrived low-key at ...
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Courreges returns to Paris, Carine Roitfeld debuts at Uniqlo
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91 shows and the jet-set Jenners: It's Paris Fashion Week
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Sophia Loren graces Armani front row at Milan Fashion Week
Giorgio Armani had the last word at Milan Fashion Week, which wrapped up six days of womenswear previews for next spring and ...
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Fashion crowd applauds US open winner Pennetta at Ferragamo
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Heidi Klum gently, but persistently cajoled her boyfriend Vito Schnabel buy her a giant plastic snail at the 7th annual Milan ...
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Peter Dundas underlines brand's ease in Cavalli debut
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Versace launches camo-glam looks for next summer
Light and transparent fabrics — from organza to lace to netting — dominated the shows Friday at Milan Fashion Week.
September 24, 2015
Massimo Giorgetti makes Pucci debut
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All Hail Athens - Go Athena!!!
... Greek Parthenon, which part of it was plaster to protect it from harm. After we explored the Parthenon we went to the museum that had the missing relics, which were very beautiful. They had a smaller model of the Parthenon made out of Legos. After, we decided to explore the overcrowded town. As we were walking a pickpocket almost mugged Mom. We looked around some shops. I got ...
Ancient Athens
... crossed the road to see the temple of Zeus ( or what remains of it) and look up to the view of the Acropolis, high on the hill. It was 28 degrees this afternoon as we were walking around so still pretty warm. We met back up with the tour group at the designated time to get back on the bus and catch our ride back to the ship. We spent the afternoon lazing about and catching up on a few ...
I don't wanna cause no fuss, jump on the magic bus
... I could take in heat, one of us likes the heat more than the other ;) Jess is doing a helluva job taking pics and documenting our trip. Following the beach we waited for the bus to pick us up again for about 40 minutes while we just took in the view and drank very very warm water. We then took our bus ride and metro ride back to our area of Athens and crushed a cold water and a sparkling lemonade for TB! Poco poco for eats tonight before we ...
Athens' Achilles heel(s) and then some
... decided against the High Tea (with a glass of bubbles); had a lemon eclair with chocolate sauce and a hot chocolate (probably would have been better value to have had the High Tea but it looked like it would have needed to have been shared; not the glass of bubbles, mind you!) Had my retail therapy hit at Attica, a fairly up-market department store; then wandered along some of streets of the Plaka - very interesting array of shops...can only ...
Acropolis Apocalypse
... being a fantastic view looking down on the Acropolis. On reaching the top we were rewarded with a real panoramic view of the whole of Athens. As we trekked back down again we stopped to chat to an American single traveller who also had become addicted to snapping the Acropolis - now, she was older than us but her memory and knowledge of Greek history (and beyond) put us to shame - and she didn't stop talking, we stood listening for an hour and learnt quite a ...


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India has extended the benefit of nil custom and production tax on bunker fuels to domestic coastal movement of containers, in a move to decongest its roads and reduce carbon emissions,
Simrad HALO Pulse Compression Radar has been awarded the Technology Award at the 2015 National Marine Electronics Association (NMEA) Conference and Expo in Baltimore, Md.
Sanmar Shipyard of Turkey has recently completed two RAscal 1500 tugs for Jordanian owners. The tugs are now delivering marine services to the FSRU berthing
Today, at the ITS World Congress and Exhibition, Xerox and Mitsubishi Heavy Industries (MHI), both leaders in transportation solutions, signed a Memorandum of Understanding to explore,
Sociedad Puerto Industrial de Aguadulce S.A. (SPIA), a unit of International Container Terminal Services, Inc. (ICTSI) in Colombia, recently received four super


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CES Report, Part 2: The Highlight Reel
For photographers, a few bright spots
There were several innovative, darn-near-revolutionary photo products introduced at CES 2010, but if you scanned consumer electronics web sites over the last week or so, you would hardly know it. Let's look at key photo products and trends that were overlooked because they weren't 3D TV, new gaming devices, tablet computers, or new competitors to the iPhone.
A new chapter for APS sensors
With the long-awaited introduction of the Samsung NX-10, a new chapter opened for the APS sensor. APS sat forlornly on the sidelines last year as Olympus and Panasonic, amid much ballyhoo, announced competing mirrorless interchangeable-lens cameras built around their pint-sized Micro Four Thirds sensor. Four Thirds is smaller than APS and so image quality has its limits, but APS sensors have the potential to deliver big blow-up prints at higher ISOs, and with Samsung's announcement APS finally has the chance to strut its stuff in a smaller body.
As with Micro Four Thirds cameras, the NX-10 replaces the space-eating mirror prism or pentaprism optical finder with an electronic viewfinder showing a live image. Without the optical prism's bulk, the camera body can be slimmed down. A new line of small lenses that accommodate a shorter flangeback (distance between the rear of the lens and sensor surface) has been introduced in a new mount, although an adapter for Samsung/Pentax SLR lenses is promised.
The only question now is not if, but when the next major DSLR maker jumps on this "mini APS" bandwagon. I'm betting we'll see more next month at PMA.
Kodak gets it
Kodak has spent several awkward years trying to transform itself from a film-and-darkroom company to a high-tech digital powerhouse. At this CES, Kodak introduced the Playsport, Pulse, and Slice—definitely cutting edge-sounding products. The Playsport is a waterproof, pocket-sized HD camera that was named by Kodak's Twitter followers and Facebook fans. The Pulse is a touch-sensitive digital frame with Wi-Fi connectivity and 4,000 image capacity. The Slice (shown) is a 14MP camera that can capture 720p HD videos and is operated via a touchscreen. Say what you will about the excessive megapixel count, the Slice represents a big jump into high-tech cameras.
My take on Kodak at CES is that it has become more Internet savvy and is using social networks to become more attuned to its customers. They introduced more relevant and eye-catching consumer products, which hopefully will help boost sales. After several years of wandering in the digital wilderness (with the slow sales to show it), Kodak is doing it right, and may have found the right path.
Polaroid apologizes
It takes a big company to admit that it screwed up. The outpouring of grief and anger over the last couple of years in the wake of Polaroid's discontinuing its instant film apparently got through. While the current owners of the Polaroid name didn't say it in so many words, their announcement of the imminent return of Polaroid Color 600 film and a new OneStep camera has been joyously accepted Polaroid fans as an apology.
Polaroid's owners couldn't help but notice that Fujifilm stepped into the huge vacuum they left by bringing the Instax line of instant film cameras into the US. They might have felt pressure from a group of former employees, the Impossible Project, which worked, with some success, to revive Polaroid instant film by purchasing a former Polaroid facility in the Netherlands. Or perhaps the thousands of angry forum posts and discussions by the niche market they abandoned shamed them into it. The result is what counts: Polaroid Instant film and cameras are coming back, and the people are happy. Non-scientific evidence to confirm this is the fact that my Tweet announcing the return of Polaroid was one of the most popular I've ever posted.
It's in the (memory) cards
Memory cards continue to get faster and hold more data. Lexar and Panasonic led the way. Lexar announced 300x CF cards in 32GB capacity and 233x cards in 16 and 32GB, while its 133x cards now reach Class 10 transfer speeds. This is very good news for video recording, as the cards will record more video time, faster. Panasonic unveiled the first SDXC format cards, an expansion in speed and capacity of the SD format. Its new Class 10 cards come in 48 and 64GB capacities. That's enough to hold up to 8.5 hours of 1080i HD video.
Sony surprised some by introducing a line of five SD and SDHC memory cards and left some wondering if the electronics behemoth is moving away from its proprietary, pricier MemoryStick card format. Reaction from my Twitter stream? "I hope so" and "It's about time" were typical. Perhaps Sony realizes that it is limiting sales by forcing people to use an unpopular storage card.
One lonely DSLR
The sole DSLR introduced at CES came from Sony—the Alpha 450, which seems to be a "high-end basic" DSLR, which means it's being marketed to and designed for first-time DSLR buyers, but it boasts some mid-range DSLR features. For instance, it has a 7fps burst rate, a feature most first-timers wouldn't appreciate, and built-in HDR, a feature that enthusiasts would understand but beginners may not. 14MP resolution is fine for big prints, but most step-up shooters don't need that much and in fact, the big files produced could gum up their computer's processing power. In other words, the A450 looks like a very capable camera, but it has a bit of an identity problem.
Canon becomes a little fish in a big pond
For items of interest to serious photographers, the pickin's were slim: Canon, which will not be at PMA this year, had plenty of new products, but very little of interest for serious photographers. They announced a replacement for their 70-200mm pro lens and wireless transmitters for their high-end DSLRs—not the sort of stuff that will get much of a spotlight at a show like CES.
Canon did announce a new line of Vixia camcorders http://www.adorama.com/news/Canon-launches-new-Vixia-camcorder-line that record videos onto SD memory cards—which is good and appropriate for CES—and four basic compact still cameras. But unlike PMA, where Canon was one of the biggest and most prominent exhibitors, at CES it was just another company vying for attention.
Megapixel madness—still?
I give Canon credit for not going overboard with pixel density on their compact cameras, limiting their highest resolution to 12MP, which is already more pixels than necessary. But apparently, Casio, Kodak, Olympus, Panasonic, GE, Sony, Samsung and even Vivitar didn't get the memo. All of them introduced at least one 14MP camera, even though we've established in so many ways that squeezing that many pixels on a pinkie nail-sized sensor will cause artifacts that will adversely affect overall image quality.
I've said this before, and I'll say it again: If you're like 95 percent of the population, the largest print you're going to make is 4x6, maybe an occasional 5x7. For that, all you need is a 6MP camera, and even if you plan on making 8x10s, anything above 8MP is overkill. Shop for features and performance, not pixels.
But this is the mass-market mentality that CES feeds into: More megapixels are better. What a shame that so many people, dazzled by the pixel count, will be disappointed with their image quality while their image files become unmanageably huge.
Final words
If CES proved anything from the perspective of the photography industry this year, it is that CES is not a photography show. Digital photography products may have been in the CES spotlight for a few years, but this year some innovative cameras were ignored for the sake of flashier gadgets. The public has moved on.
I note that there were no new product announcements from hardcore photo companies such as Nikon, Sigma, Tamron, Tokina, or Pentax, although I suspect we'll hear plenty from them in Anaheim next month.
As I said on Friday, there has been some discussion in the industry of folding PMA into CES, and because of the sheer size of the show and the fickle nature of the consumer electronics world, I think this would be a very bad idea. Keep PMA strong and give photography a stage where it will continue to be the star. Perhaps letting the general public in on the last day of the show could give the show a boost
What was your biggest CES news? Any disappointments? Leave a comment!
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text: Chas Vincent Montana Lawmakers Critical Of New 'Waters of the U.S.' Rules By Steve Jess • Jun 1, 2015 Listen Listening... / 1:56 Montana Lawmakers Critical Of New 'Waters of the U.S.' Rules Monday at the Montana Capitol the Legislature's Water Policy Interim Committee raised questions about new federal clean water rules intended to give the Environmental Protection Agency a say in regulating many streams and ditches that are now the domain of state and local regulators. Montana lawmakers from both parties say they're troubled by the new rules. Lawmakers Debated Flathead Compact Saturday, Vote Monday By Steve Jess • Apr 12, 2015 Flickr, Peggy2012CREATIVELENZ Listen Listening... / 5:06 Hear some of the dozens of people who came to Helena Saturday to argue for and against the Flathead water compact. The Montana State Capitol was bustling with activity Saturday, as literal busloads of people arrived from the Flathead Valley to weigh in on the controversial Flathead Reservation compact that will settle tribal water rights and determine how much water farmers and ranchers of that region will receive in future years. Flathead Water Compact On Saturday's Legislative Agenda By Madelyn Beck - UM Legislative News Service • Apr 10, 2015 William Marcus Listen Listening... / 1:00 Flathead Water Compact On Saturday's Legislative Agenda The Montana Legislature is holding a special weekend hearing Saturday for the hundreds expected to come to speak their mind on the Salish Kootenai water compact. If passed, the compact would outline water rights on and around the Flathead Reservation. Veto Likely For Two Gun Bills Passed By State Senate By Steve Jess • Mar 11, 2015 Webmaster 102 (CC-BY-3.0) The Montana Senate Wednesday passed two gun-rights bills that are virtual copies of bills the Governor vetoed in the last session. Libby Republican Chas Vincent presented his bill to eliminate the need for gun owners to obtain a concealed-carry permit. Vincent says it's a minor change, since permits have not been needed outside city limits in Montana for over two decades. "Since 1991, everybody's been able to carry concealed outside of city limits. So for 24 years, and there hasn't been any data to suggest that that has been a bad policy," said Vincent. 'Capitol Talk' Reviews This Week At The Montana Legislature By Sally Mauk • Feb 27, 2015 Eliza Wiley Listen Listening... / 12:19 'Capitol Talk' Reviews This Week At The Montana Legislature This week on Capitol Talk: Sally, Mike and Chuck look back at the week's events at the Montana Legislature, from the Flathead water compact, to dark money, to the death of the death penalty repeal. Flathead Compact Survives The Senate By Steve Jess • Feb 25, 2015 William Marcus Supporters of the proposed Flathead Water Compact, involving the state, the federal government, and the Confederated Salish and Kootenai Tribes have a victory to celebrate. The compact, one of the most contentious issues of the current Legislative session not only survived a debate and vote in the Montana Senate, but did so with a sizable margin. Flathead Water Compact Gets Resurrected After Successful 'Blast' Motion By Steve Jess • Feb 24, 2015 Montana Legislature The Flathead Water Compact working its way through the Montana Legislature was briefly killed today, but quickly brought back to life. Because the massive water-rights agreement contains $8 million for canal system upgrades, the bill was routed to the Senate finance Committee. There, Dayton Republican Janna Taylor tried to amend it to add financial accountability. The Sponsor Republican Chas Vincent, saw ulterior motives. Flathead Water Compact Advances To Senate Floor After Contentious Debate By Steve Jess • Feb 20, 2015 The Montana Legislature took a step toward ratifying the state's final outstanding water compact this morning, with a vote in the Senate Judiciary Committee. The compact involving the federal government and the Confederated Salish and Kootenai Tribes has drawn more heated debate than any issue except perhaps Medicaid expansion. Flathead Water Compact Faces First Committee Vote Friday By Madelyn Beck - UM Legislative News Service • Feb 19, 2015 William Marcus On Friday, the Salish-Kootenai water compact faces its first big vote. If the Senate Judiciary Committee advances the bill, it will face the anger of irrigators who say they won't get as much water. Dozens Testify At Flathead Water Compact's First Hearing By Steve Jess • Feb 16, 2015 Eric Whitney On the calendar it may have been Presidents Day, but for the Senate Judiciary Committee, Monday was no holiday. Flathead Water Compact On Monday's Legislative Agenda By Madelyn Beck - UM Legislative News Service • Feb 13, 2015 William Marcus Monday, the Montana legislature will hear the bill to pass the Salish-Kootenai water compact, essentially mapping out water rights for the Flathead reservation and surrounding area. The bill's sponsor is Republican Senator Chas Vincent of Libby, who was the compact's opposition last session. He says, even amid death threats, he believes this year's compact is better and absolutely necessary to ensure Montana keeps it's water. Libby Lawmaker Educates Colleagues On Flathead Water Compact By Steve Jess • Feb 9, 2015 Steve Jess This past weekend, about 40 of Montana's state legislators did something unusual. After a very busy week, they stuck around when they could have gone home. They spent close to three hours on a Saturday morning in the old Supreme Court chamber of the capitol, at the invitation of Republican Senator Chas Vincent of Libby. 'Capitol Talk' Recaps This Week At The Montana Legislature By Sally Mauk • Feb 6, 2015 Eliza Wiley This week on "Capitol Talk", tax cut bills are getting a lot of attention at the Montana Legislature this session. One legislator, Nicholas Schwaderer of Superior, introduced a bill that would put income tax cuts up to a vote via referendum. The bill drew bipartisan opposition during its hearing in the House. Schwaderer defended the bill saying, "taxation of income is theft." Tax cuts could face vetoes from Gov. Bullock, who is intent on keeping at least a $300 million surplus. More asking for re-negotiation on Flathead Water Compact By Dan Boyce • Mar 24, 2014 Flickr, Peggy2012CREATIVELENZ More are calling for a re-opening of negotiations on the Flathead Water Compact, soon possibly including the state commission which helped craft the compact. But the Confederated Salish and Kootenai tribes are holding firm with the current version. The CSKT have been working on this agreement with the state and federal governments and private irrigators for at least a dozen years. It would settle disputes over how water is shared on the Flathead reservation. Lawmakers, public still divided on Flathead water compact By Dan Boyce • Jan 8, 2014 Flickr, Peggy2012CREATIVELENZ A committee of state lawmakers said this week they still don't they have enough information to recommend passage of the long-awaited Flathead Water Rights Compact. The Montana Department of Natural Resources and Conservation submitted a report to the Water Policy Interim Committee which was intended to answer lawmaker's questions about the compact after the document was tabled by the 2013 Legislature. Committee Chairman Sen. Chas Vincent (R-Libby) said the report did not fully accomplish that goal.


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EMD: an ensemble algorithm for discovering regulatory motifs in DNA sequences
1 Department of Computer Science, Purdue University, West Lafayette, IN, 47907, USA
2 Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA
3 Markey Center for Structural Biology, Purdue University, West Lafayette, IN, 47907, USA
4 The Bindley Bioscience Center, Discovery Park, Purdue University, West Lafayette, IN, 47907, USA
BMC Bioinformatics 2006, 7:342 doi:10.1186/1471-2105-7-342Published: 13 July 2006
Understanding gene regulatory networks has become one of the central research problems in bioinformatics. More than thirty algorithms have been proposed to identify DNA regulatory sites during the past thirty years. However, the prediction accuracy of these algorithms is still quite low. Ensemble algorithms have emerged as an effective strategy in bioinformatics for improving the prediction accuracy by exploiting the synergetic prediction capability of multiple algorithms.
We proposed a novel clustering-based ensemble algorithm named EMD for de novo motif discovery by combining multiple predictions from multiple runs of one or more base component algorithms. The ensemble approach is applied to the motif discovery problem for the first time. The algorithm is tested on a benchmark dataset generated from E. coli RegulonDB. The EMD algorithm has achieved 22.4% improvement in terms of the nucleotide level prediction accuracy over the best stand-alone component algorithm. The advantage of the EMD algorithm is more significant for shorter input sequences, but most importantly, it always outperforms or at least stays at the same performance level of the stand-alone component algorithms even for longer sequences.
We proposed an ensemble approach for the motif discovery problem by taking advantage of the availability of a large number of motif discovery programs. We have shown that the ensemble approach is an effective strategy for improving both sensitivity and specificity, thus the accuracy of the prediction. The advantage of the EMD algorithm is its flexibility in the sense that a new powerful algorithm can be easily added to the system.


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FILM; Three Films, Three Genres, One Work
By DAVE KEHR
Published: January 25, 2004
NOT just for Greek tragedies any more, the trilogy has become the all-but-obligatory format for major franchise films. Both ''The Matrix'' and ''The Lord of the Rings'' had grand finales last year, packing them in at multiplexes around the world.
But trilogies have been turning up in the art houses, as well, in part because of the critical and commercial success of Krzysztof Kieslowski's ''Three Colors'' series of 1993 and 1994. Kieslowski, a Polish filmmaker then based in Paris, worked through three apparently unrelated tales, each named for a color of the French flag: ''Blue,'' ''White'' and ''Red.'' As the trilogy unfolded, it revealed a complex internal structure, based on the repetition of themes and the rhyming of images.
Kieslowski died before he could complete a planned second trilogy, based on the Catholic accounts of heaven, hell and purgatory, though the German director Tom Twyker made ''Heaven'' (2002) from the screenplay by Kieslowski and his frequent collaborator, Krzysztof Piesiewicz. Rebecca Miller's one-film trilogy, ''Personal Velocity,'' also in 2002, packed parallel stories of three women's search for freedom into one feature. Lars von Trier's Danish film ''Dogville,'' which will be released in America in March, is the first panel in a planned triptych on European visions of American life. Mr. von Trier says he will continue the series even though the star of ''Dogville,'' Nicole Kidman, has dropped out of the next two.
Beginning this week in New York (with a national release to follow) is Lucas Belvaux's series ''The Trilogy'' (''La Trilogie),'' a great critical success in France when it was released in 2002. The film's three feature-length installments -- titled, in the order they will be shown in the United States, ''On the Run,'' ''An Amazing Couple'' and ''After Life'' -- are all set in the mountain city of Grenoble, where the three female protagonists work in a high school. The women and their husbands, lovers and rivals, cross over from film to film, though each movie has its own story and, more particularly, its own genre.
''On the Run'' (opening on Friday) is a political thriller, in which Mr. Belvaux (long a professional actor) plays a militant radical who escapes from prison and forces an old comrade, a schoolteacher played by Catherine Frot, to help him plan a new series of bombings. In ''An Amazing Couple'' (opening on Feb. 6), the tone shifts to something approaching screwball comedy for the tale of a Grenoble industrialist (François Morel) whose wife (Ornella Muti) mistakenly believes he is having an affair. For the final film, ''After Life'' (opening on Feb. 13), Mr. Belvaux shifts to a more intimate, naturalistic tone for a domestic drama about the tortured relationship of a corrupt police inspector (Gilbert Melki) and his morphine-addicted wife (Dominique Blanc) who is harboring the radical.
The stories take place simultaneously, with events seen from different perspectives in different films and major characters in one film becoming supporting players in another.
''It began with a theoretical question,'' Mr. Belvaux, speaking French, said during a recent visit to New York. ''What is a secondary character? When I was making my first film, 'Parfois Trop d'Amour' in 1992, I thought I had three very sympathetic leading characters, but I realized that from the point of view of one of the secondary characters, they were really quite antipathetic. I thought then of making a series of films in which the characters would change their stature -- principals become secondary and supporting characters become principals. At the time, I was reading lots of Balzac, and in his novels you see the same thing: characters who are important in one story but who appear as background figures in another. It is all a question of point of view.''
Where in the past many Hollywood trilogies -- Francis Ford Coppola's ''Godfather Saga,'' for example, or the ''Die Hard'' movies -- seemed to become so after the fact, driven by commercial demands for sequels, the recent European trilogies have been imagined from the very beginning as single unified works (as were, of course, ''The Matrix'' and ''The Lord of the Rings,'' and George Lucas has said that he planned the ''Star Wars'' series as three interlocking trilogies from the start). For Mr. Belvaux, the configuration made inherent sense; paraphrasing Aristotle on the principle of the golden mean, he says: ''Two is too little and four is too much. And also, three gives a sense of unbalance, of irresolution, where an even number is too symmetrical, it suggests a conclusion.''
Mr. Belvaux, a pleasant, rumpled 42-year-old, began writing ''The Trilogy'' shortly after he completed his second feature, ''Pour Rire'' (''For Laughs''), in 1996. ''The three films advanced in parallel,'' he said. ''Roughly, I worked about a week on the global project, defining the idea of the three principal couples who would pass through three different genres. I needed to find a way to cross the three stories without making it seem too forced, so I settled on the idea that if the three women were friends and all worked in the same place, necessarily the stories would intersect.


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Report NEP-CTA-2014-04-29This is the archive for NEP-CTA, a report on new working papers in the area of Contract Theory & Applications. Simona Fabrizi issued this report. It is usually issued weekly.
Subscribe to this report: email, RSS or Twitter.
Other reports in NEP-CTA
The following items were announced in this report:
- Ulbricht, Robert, 2014. "Optimal Delegated Search with Adverse Selection and Moral Hazard," TSE Working Papers 14-475, Toulouse School of Economics (TSE).
- Christoph Kuzmics, 2014. "A full characterization of all deterministic dominant strategy incentive compatible, ex-post individually rational, and ex-post budget balanced direct mechanisms in the public good provision problem w," Center for Mathematical Economics Working Papers 504, Center for Mathematical Economics, Bielefeld University.
- Dirk Bergemann & Benjamin Brooks & Stephen Morris, 2013. "The Limits of Price Discrimination," Cowles Foundation Discussion Papers 1896RR, Cowles Foundation for Research in Economics, Yale University, revised Apr 2014.
- Martin Kuncl, 2014. "Securitization under Asymmetric Information over the Business Cycle," CERGE-EI Working Papers wp506, The Center for Economic Research and Graduate Education - Economics Institute, Prague.
- Aanesen, Margrethe & Czajkowski, Mikolaj & Falk-Peterson, Jannike & Hanley, Nicholas & LaRiviere, Jacob & Tinch, Dugald, 2014. "Effects of Experience, Knowledge and Signals on Willingness to Pay for a Public Good," Stirling Economics Discussion Papers 2014-04, University of Stirling, Division of Economics.
- Andrea Attar & Thomas Mariotti & François Salanié, 2014. "On Competitive Nonlinear Pricing," CEIS Research Paper 314, Tor Vergata University, CEIS, revised 18 Apr 2014.
- Czajkowski, Mikolaj & Hanley, Nicholas & LaRiviere, Jacob & Simpson, Katherine, 2014. "What is the Causal Effect of Information and Learning about a Public Good on Willingness to Pay?," Stirling Economics Discussion Papers 2014-05, University of Stirling, Division of Economics.
- Hatchondo, Juan Carlos & Krusell, Per & Schneider, Martin, 2014. "Asset Trading and Valuation with Uncertain Exposure," Working Paper 14-5, Federal Reserve Bank of Richmond.
- Czarnitzki, Dirk & Hall, Bronwyn H. & Hottenrott, Hanna, 2014. "Patents as quality signals? The implications for financing constraints on R&D," ZEW Discussion Papers 14-023, ZEW - Zentrum für Europäische Wirtschaftsforschung / Center for European Economic Research.
- Cogley, Timothy & Matthes, Christian & Sbordone, Argia M., 2014. "Optimized Taylor Rules for Disinflation When Agents are Learning," Working Paper 14-7, Federal Reserve Bank of Richmond.
- Zuazo Garín, Peio, 2014. "Uncertain Information Structures and Backward Induction," IKERLANAK 12097, Universidad del País Vasco - Departamento de Fundamentos del Análisis Económico I.
- Seungjin Han, 2014. "Sellers' Implicit Collusion in Directed Search Markets," Department of Economics Working Papers 2014-05, McMaster University, revised Sep 2015.
- Schnedler, Wendelin & Vanberg, Christoph, 2014. "Playing 'Hard to Get': An Economic Rationale for Crowding Out of Intrinsically Motivated Behavior," IZA Discussion Papers 8108, Institute for the Study of Labor (IZA).
- Roig, Guillem, 2014. "Competition and the Hold‐U p Problem: a Setting with Non‐exclusive Contracts," TSE Working Papers 14-481, Toulouse School of Economics (TSE).
- Björn Bartling & Nick Netzer, 2014. "An externality-robust auction: theory and experimental evidence," ECON - Working Papers 153, Department of Economics - University of Zurich.
- Josef Falkinger, 2014. "In search of economic reality under the veil of financial markets," ECON - Working Papers 154, Department of Economics - University of Zurich.


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We are planning a vaginal birth. I know that sometimes baby's head gets a little smushed in the vaginal birth process and comes out a shaped a little like a cone. Knowing this, I've been preparing for a cone head baby by buying a lot of cute hats, so that my little's one's first pictures are still super cute. I mean, I expect they would be anyway, but you know what I mean.Ā
Anyway, the other day I was talking with my mom and aunt about this, and my aunt acted like I was crazy. ĀShe seems to think that if it didn't happen with her kids that it just doesn't ever happen, which you know, is silly and I know better.Ā
However, it did get me to thinking...am I being ridiculous? I mean, I've SEEN cone headed babies before, so I know it happens, but I guess I don't know how often it happens.
So, I'm posing the question to you lovely ladies. Have you had a cone headed baby? Have you seen them? Do you have any idea how often this kind of thing happens?Ā
For the record, the hats will be used regardless. We live in Thailand. It's really freaking hot and sunny here. Our baby will likely be wearing hats to keep the sun out her eyes and off her face regardless of the shape of her head.


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A mechanical engineer by education, I'm currently working for HP's
Large Format Printing Specialty Printing Systems Advanced Platforms Group in Barcelona, Spain, San Diego, CA, dealing mostly with printing algorithms computer vision firmware.
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My nickname Miki i am from thailand i love vocaloid music facebook https://www.facebook.com/profile.php?id=100007033843088 18 year old slogan Every layer is my idol .
Hi I am SquallV3, I am a cosplayer, gamer and aspiring martial artist. I got into cosplay thanks to my beautiful and very talented wife, who gives me my ideas and inpirations for the cosplay outfits and who also is my seamstress. FB page: www.facebook.com/Kalikittycosplay Youtube: www.youtube.com/channel/UCYk6ecropZDpMbsM3N5uwsw
Proud Carioca. Cosplayer since 2011. K-POP Addicted. Anime Lover. #DeviantArt: http://pabas.deviantart.com/ #Tumblr: http://nankaa.tumblr.com/ #Ample: http://ample-cosplay.com/?u=0000002672
No soy nadie~ http://alicemmh.deviantart.com/ http://www.pixiv.net/member.php?id=3664480 https://www.youtube.com/user/alicemmh
Hi... I love made cosplay because for me it is full creativity
Currently residing in Texas, The primary Smiths of the Falamangosa Props Armory, Taylor and her Husband Kristoff fabricate and model their armor's across Austin and the state. Going from props and armor made for others, to wearing and promoting their stuff was an interesting turn of events, but worth it entirely. The exhilaration from wearing something that makes people stop in awe cant be beat.
Hi, I love Cartoon and Anime, Nice to meet you.
Fotografos: Marcos Vinicius João Victor Produtora: Cinthya Solaira Tatiana Travassos Cosmodel: Nick j ( Kaede Chan ) Luciana Lopes ( Asuna ) Gisele Solaira Nathália Prado ( Naty Teshii ) Tatiana Travassos ( Lindinha ) Editores: Gisele João Victor Marcos Vinicius Camilla Barçante Tatiana Travassos Designer: Camilla Barçante E-mail: email@example.com Facebook: www.facebook.com/mphotos Flick: /www.flickr.com/photos/128339694@N07/
Hello, Im M.J Nepacena ^_^ feel free to add me or follow me on facebook :) i love chatting ^_^ lets be friends :) 【FB】: https://www.facebook.com/deathscythe146 【FB page】:https://www.facebook.com/MJSieghart146 i hope we could be good friends ^_^
Hi o/ My name is Gii and I'm a brazilian cosplayer since July 2013 I hope you enjoy my work >__< Thanks for visiting, follow and likes ♣ Instagram: http://instagram.com/gii_oliveira_ ♣ Facebook Page: https://www.facebook.com/GiiCosplay ♣ CosGamersBrasil: http://www.cosgamersbrasil.com.br/giioliveira/ ♣ Tumblr: http://a--normalize.tumblr.com
Hi! I'm Andy, 22 years old, Brazilian cosplayer! I have a facebook page! Check it out! https://www.facebook.com/pages/AndyEddie/321369548059874 "...Façamos da interrupção um caminho novo. Da queda um passo de dança, do medo uma escada, do sonho uma ponte, da procura um encontro!.." Fernando Sabino
おはよう みなさん! I'm StarDust★Blader I'm a Filipino-Canadian cosplayer from Montréal, QC, Canada. I'm a rookie with a BIG dream ^^ I believe EVERYONE deserves to cosplay :3 •FB: https://www.facebook.com/TSUKINoStarDustCosplay •Deviant: http://stardustdrivedragon.deviantart.com/ •ASK: http://ask.fm/StarDustBlader •WC: http://worldcosplay.net/member/307164 •WC: http://worldcosplay.net/member/356527 •Tumblr: https://www.tumblr.com/blog/stardustblader •AMINO: https://aminoapps.com/p/yk914


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Poll: I've been publishing some Non-Harry Potter stuff on an alternate account. Should I move them to this account? Vote Now!
Author has written 9 stories for Harry Potter, and X-overs.
Rune Reader Progress Update
Currently on Chapter 13 in the writing. I'll probably post the next chapter, which would be chapter 9 by my numbering system, the next opportunity I get.
Concerning a schedule: I told myself I'd stop this "Update every 2 months and 2 chapters at a time" bullshit, but then I somehow decided that the next few chapters would push over 5k words, so...
Personal Notes
1. Everyone who's ever read any of my stories probably knows that I am greatly inspired by music. Those inspirations come at strange times sometimes, and one of my ways of citing these inspirations is that my chapter titles and some of my dialogue is often lifted from some of the songs, usually the ones that inspired the chapters/lines.
2. Tobias Sammet's Avantasia might be my favorite band of all time, and I've yet to discover a song I don't like off any of their albums. Thus, Avantasia is a near constant source of inspiration for my stories, and a number of plot devices or character titles have their origin in Avantasia (i.e. The Dweller, Savior in the Clockwork, Battle of the Seven Angels, etc.)
3. That being said, one might think I like solely Power Metal and Classic Rock (as I often use CR songs as inspirations). This is not the case, as I've discovered I like music from across a number of genres, including Rap, Country and Dubstep.
4. Originally, I cited a number of Animes that were also sources for ideas in some of my stories. However, my more recent stories do not have this, as I've discovered that my personal dislikes in a number of Anime now surpass what I like about them.


text: previous next Fullscreen Music • June, 16 2013 • By David Fischer Pharrell Remixes Woodkid's "I Love You" Shares Share Tweet Email Reddit Flipboard WhatsApp Comments Pharrell produced a remix of the recent Woodkid single "I Love You." With only very minimal changes to the original, choosing a favorite might be difficult. You can listen to the new version now here below. Filed under: Pharrell Williams, woodkid


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Are you Bunzo Mikami?Claim your profile
Publications (225)662.27 Total impact
Article: Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate[Show abstract] [Hide abstract]
ABSTRACT: The acidic polysaccharide alginate represents a promising marine biomass for the microbial production of biofuels, although the molecular and structural characteristics of alginate transporters remain to be clarified. In Sphingomonas sp. A1, the ATP-binding cassette transporter AlgM1M2SS is responsible for the import of alginate across the cytoplasmic membrane. Here, we present the substrate-transport characteristics and quaternary structure of AlgM1M2SS. The addition of poly- or oligoalginate enhanced the ATPase activity of reconstituted AlgM1M2SS coupled with one of the periplasmic solute-binding proteins, AlgQ1 or AlgQ2. External fluorescence-labeled oligoalginates were specifically imported into AlgM1M2SS-containing proteoliposomes in the presence of AlgQ2, ATP, and Mg(2+). The crystal structure of AlgQ2-bound AlgM1M2SS adopts an inward-facing conformation. The interaction between AlgQ2 and AlgM1M2SS induces the formation of an alginate-binding tunnel-like structure accessible to the solvent. The translocation route inside the transmembrane domains contains charged residues suitable for the import of acidic saccharides. Copyright © 2015 Elsevier Ltd. All rights reserved.Structure 07/2015; 23(9). DOI:10.1016/j.str.2015.06.021 · 5.62 Impact Factor
Article: Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase[Show abstract] [Hide abstract]
ABSTRACT: Moloney murine leukemia virus reverse transcriptase (MMLV RT) contains fingers, palm, thumb, and connection subdomains as well as an RNase H domain. The DNA polymerase active site resides in the palm subdomain, and the RNase H active site is located in the RNase H domain. The RNase H domain contains a positively charged α-helix called the C helix (H(594)GEIYRRR(601)), that is thought to be involved in substrate recognition. In this study, we expressed three versions of the RNase H domain in Escherichia coli, the wild-type domain (WT) (residues Ile498-Leu671) and two variants that lack the regions containing the C helix (Ile593-Leu603 and Gly595-Thr605, which we called ΔC1 and ΔC2, respectively) with a strep-tag at the N-terminus and a deca-histidine tag at the C-terminus. These peptides were purified from the cells by anion-exchange, Ni(2+) affinity, and Strep-Tactin affinity column chromatography, and then the tags were removed by proteolysis. In an RNase H assay using a 25-bp RNA-DNA heteroduplex, WT, ΔC1, and ΔC2 produced RNA fragments ranging from 7 to 16 nucleotides (nt) whereas the full-length MMLV RT (Thr24-Leu671) produced 14-20-nt RNA fragments, suggesting that elimination of the fingers, palm, thumb, and connection subdomains affects the binding of the RNase H domain to the RNA-DNA heteroduplex. The activity levels of WT, ΔC1, and ΔC2 were estimated to be 1%, 0.01%, and 0.01% of full-length MMLV RT activity, indicating that the C helix is important, but not critical, for the activity of the isolated RNase H domain. Copyright © 2015. Published by Elsevier Inc.Protein Expression and Purification 05/2015; 113. DOI:10.1016/j.pep.2015.04.012 · 1.70 Impact Factor
Article: Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein[Show abstract] [Hide abstract]
ABSTRACT: β-Conglycinin is a major seed storage protein in soybeans, which are an important source of protein. The major subunits (α, α′ and β) of β-conglycinin are sorted to protein-storage vacuoles in seed cells. Vacuolar sorting receptor (VSR) is an integral membrane protein that recognizes the sorting determinant of vacuolar proteins, including β-conglycinin, and regulates their sorting process. Vacuolar sorting determinants of the α′ and β subunits of β-conglycinin exist in their C-terminal peptides. Here, the preliminary X-ray diffraction analysis of the binding domain of soybean VSR crystallized with the peptide responsible for the sorting determinant in β-conglycinin is reported. X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to space group P 3 1 21, with unit-cell parameters a = b = 116.4, c = 86.1 Å.Acta Crystallographica Section F Structural Biology and Crystallization Communications 02/2015; 71(Pt 2):132-5. DOI:10.1107/S2053230X14027484 · 0.53 Impact Factor
Article: Metabolic fate of unsaturated glucuronic/iduronic acids from glycosaminoglycans: Molecular identification and structure determination of streptococcal isomerase and dehydrogenase[Show abstract] [Hide abstract]
ABSTRACT: Glycosaminoglycans in mammalian extracellular matrices are degraded to their constituents, unsaturated uronic (glucuronic/iduronic) acids and amino sugars, through successive reactions of bacterial polysaccharide lyase and unsaturated glucuronyl hydrolase (UGL). Genes coding for glycosaminoglycans-acting lyase, UGL, and phosphotransferase system are assembled into a cluster in the genome of pathogenic bacteria, such as streptococci and clostridia. Here, we studied the streptococcal metabolic pathway of unsaturated uronic acids and the structure/function relationship of its relevant isomerase and dehydrogenase. Two proteins (gbs1892 and gbs1891) of Streptococcus agalactiae strain NEM316 were overexpressed in Escherichia coli, purified, and characterized. 4-Deoxy-L-threo-5-hexosulose-uronate (Dhu) nonenzymatically generated from unsaturated uronic acids was converted to 2-keto-3-deoxy-D-gluconate via 3-deoxy-D-glycero-2,5-hexodiulosonate through successive reactions of gbs1892 isomerase (DhuI) and gbs1891 NADH-dependent reductase/dehydrogenase (DhuD). DhuI and DhuD enzymatically corresponded to 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (KduI) and 2-keto-3-deoxy-D-gluconate dehydrogenase (KduD), respectively, involved in pectin metabolism, although no or low sequence identity was observed between DhuI and KduI or between DhuD and KduD, respectively. Genes for DhuI and DhuD were found to be included in the streptococcal genetic cluster, while KduI and KduD are encoded in clostridia. Tertiary and quaternary structures of DhuI and DhuD were determined by X-ray crystallography. Distinct from KduI β-barrels, DhuI adopts an α/β/α-barrel structure as a basic scaffold similar to that of ribose 5-phosphate isomerase. The structure of DhuD is unable to accommodate the substrate/cofactor, suggesting that conformational changes are essential to trigger enzyme catalysis. This is the first report on the bacterial metabolism of glycosaminoglycans-derived unsaturated uronic acids by isomerase and dehydrogenase. Copyright © 2015, The American Society for Biochemistry and Molecular Biology.Journal of Biological Chemistry 01/2015; 290(10). DOI:10.1074/jbc.M114.604546 · 4.57 Impact Factor
Article: Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase from Mesorhizobium loti[Show abstract] [Hide abstract]
ABSTRACT: 5-Formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase (FHMPCDH) from Mesorhizobium loti is the fifth enzyme in degradation pathway I for pyridoxine. The enzyme catalyzes a dismutation reaction: the oxidation of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) to 3-hydroxy-2-methylpyridine 4,5-dicarboxylic acid with NAD(+) and reduction of FHMPC to 4-pyridoxic acid with NADH. FHMPCDH belongs to the l-3-hydroxyacyl-CoA dehydrogenase (HAD) family. The crystal structure was determined by molecular replacement and refined to a resolution of 1.55Å (R-factor of 16.4%, Rfree=19.4%). There were two monomers in the asymmetric unit. The overall structure of the monomer consisted of N- and C-terminal domains connected by a short linker loop. The monomer was similar to members of the HAD family (RMSD=1.9Å). The active site was located between the domains and highly conserved to that of human heart l-3-hydroxyacyl-CoA dehydrogenase (HhHAD). His-Glu catalytic dyad, a serine and two asparagine residues of HhHAD were conserved. Ser116, His137 and Glu149 in FHMPC dehydrogenase are connected by a hydrogen bonding network forming a catalytic triad. The functions of the active residues in the reaction mechanism are discussed. Copyright © 2014 Elsevier Inc. All rights reserved.Biochemical and Biophysical Research Communications 11/2014; 456(1). DOI:10.1016/j.bbrc.2014.11.028 · 2.30 Impact Factor
Article: Atomic structure of recombinant thaumatin II reveals flexible conformations in two residues critical for sweetness and three consecutive glycine residues[Show abstract] [Hide abstract]
ABSTRACT: Thaumatin, an intensely sweet-tasting protein used as a sweetener, elicits a sweet taste at 50 nM. Although two major variants designated thaumatin I and thaumatin II exist in plants, there have been few dedicated thaumatin II structural studies and, to date, data beyond atomic resolution had not been obtained. To identify the detailed structural properties explaining why thaumatin elicits a sweet taste, the structure of recombinant thaumatin II was determined at the resolution of 0.99 Å. Atomic resolution structural analysis with riding hydrogen atoms illustrated the differences in the direction of the side-chains more precisely and the electron density maps of the C-terminal regions were markedly improved. Though it had been suggested that the three consecutive glycine residues (G142-G143-G144) have highly flexible conformations, G143, the central glycine residue was successfully modelled in two conformations for the first time. Furthermore, the side chain r.m.s.d. values for two residues (R67 and R82) critical for sweetness exhibited substantially higher values, suggesting that these residues are highly disordered. These results demonstrated that the flexible conformations in two critical residues favoring their interaction with sweet taste receptors are prominent features of the intensely sweet taste of thaumatin.Biochimie 11/2014; 106C. DOI:10.1016/j.biochi.2014.07.016 · 2.96 Impact Factor
Article: Structure-based Conversion of the Coenzyme Requirement of a Short-chain Dehydrogenase/Reductase Involved in Bacterial Alginate Metabolism[Show abstract] [Hide abstract]
ABSTRACT: The alginate-assimilating bacterium, Sphingomonas sp. strain A1, degrades the polysaccharide to monosaccharides through four alginate lyases reactions. The resultant monosaccharide, which is nonenzymatically converted to 4-deoxy-L-erythro-5-hexoseulose uronate (DEH), is further metabolized to 2-keto-3-deoxy-D-gluconate by NADPH-dependent reductase A1-R in the short-chain dehydrogenase/reductase (SDR) family. A1-R-deficient cells produced another DEH reductase, designated A1-R', with a preference for NADH. Here we show the identification of a novel NADH-dependent DEH reductase A1-R' in strain A1, structure determination of A1-R' by X-ray crystallography, and structure-based conversion of a coenzyme requirement in SDR enzymes, A1-R and A1-R'. A1-R' was purified from strain A1 cells and enzymatically characterized. Except for coenzyme requirement, there was no significant difference in enzyme characteristics between A1-R and A1-R'. Crystal structures of A1-R' and A1-R'/NAD+ complex were determined at 1.8 and 2.7 Å resolutions, respectively. Because of a 64% sequence identity, overall structures of A1-R' and A1-R were similar, although a difference in the coenzyme-binding site (particularly nucleoside ribose 2' region) was observed. Distinct from A1-R, A1-R' included a negatively charged, shallower binding site. These differences were caused by amino acid residues on the two loops around the site. The A1-R' mutant with the two A1-R-typed loops maintained potent enzyme activity with specificity for NADPH rather than NADH, demonstrating that the two loops determine the coenzyme requirement and loop exchange is a promising method for conversion of coenzyme requirement in the SDR family.Journal of Biological Chemistry 10/2014; 289(48). DOI:10.1074/jbc.M114.585661 · 4.57 Impact Factor
Article: Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity[Show abstract] [Hide abstract]
ABSTRACT: Chitinase hydrolyzes the β-1,4-glycosidic bond in chitin. In higher plants, this enzyme has been regarded as a pathogenesis-related protein. Recently, we identified a class III chitinase, which functions as a calcium storage protein in pomegranate (Punica granatum) seed (PSC, pomegranate seed chitinase). Here, we solved a crystal structure of PSC at 1.6 Å resolution. Although its overall structure, including the structure of catalytic site and non-proline cis-peptides, was closely similar to those of other class III chitinases, PSC had some unique structural characteristics. First, there were some metal-binding sites with coordinated water molecules on the surface of PSC. Second, many unconserved aspartate residues were present in the PSC sequence which rendered the surface of PSC negatively charged. This acidic electrostatic property is in contrast to that of hevamine, well-characterized plant class III chitinase, which has rather a positively charged surface. Thus, the crystal structure provides a clue for metal association property of PSC.Bioscience Biotechnology and Biochemistry 09/2014; 79(1):1-6. DOI:10.1080/09168451.2014.962475 · 1.06 Impact Factor
Article: Crystal structure of a crustacean prophenoloxidase provides a clue to understanding the functionality of the type 3 copper proteins[Show abstract] [Hide abstract]
ABSTRACT: Unlabelled: Phenoloxidase (PO), which is classified as a type 3 copper protein, catalyzes the hydroxylation of monophenol to o-diphenol and subsequent oxidation to the corresponding o-quinone. The geometry and coordination environment of the active site of the arthropod PO are very similar to those of the arthropod hemocyanin (Hc). However, unlike the POs, Hc is an oxygen carrier in crustaceans, and does not possess PO activity in general. Recently, we identified a new type of proPO from a crustacean and designated it proPOβ. This enzyme has many characteristics that are rather similar to those of Hc, such as its maturation, localization, and oligomeric state. Here, we determined the crystal structure of proPOβ prepared from the hemolymph of kuruma prawns (Marsupenaeus japonicus) at 1.8-Å resolution. M. japonicus proPOβ forms a homohexamer rather similar to that of arthropod Hc. The geometry of the active copper site in proPOβ is nearly identical to that of arthropod Hc. Furthermore, the well-characterized 'place-holder' phenylalanine is present (Phe72). However, the accessibility to the active site differs in several ways. First, another phenylalanine, which shields the active site by interacting with a copper-coordinated histidine in crustacean Hc, is replaced by valine in the proPOβ structure. Second, two tyrosines, Tyr208 and Tyr209, both of which are absent in Hc, show the alternative conformations and form a pathway providing access to the reaction center. Thus, the present crystal structure clarifies the similarities and differences in the activity of two closely related proteins, PO and Hc. Database: Structural data are available in the RSCB protein data bank under the accession number 3WKY. ray crystallography (View interaction).FEBS Journal 04/2014; 281(11). DOI:10.1111/febs.12812 · 4.00 Impact Factor
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ABSTRACT: 4-Pyridoxolactonase from Mesorhizobium loti catalyzes the zinc-dependent lactone-ring hydrolysis of 4-pyridoxolactone (4PAL) to 4-pyridoxic acid (4PA) in vitamin B 6 degradation pathway I. The crystal structures of 4-pyridoxolactonase and its complex with 5-pyridoxolactone (5PAL; the competitive inhibitor) were determined. The overall structure was an αβ/βα sandwich fold, and two zinc ions were coordinated. This strongly suggested that the enzyme belongs to subclass B3 of the class B β-lactamases. In the complex structure, the carbonyl group of 5PAL pointed away from the active site, revealing why it acts as a competitive inhibitor. Based on docking simulation with 4PAL, 4PA and a reaction intermediate, 4-pyridoxolactonase probably catalyzes the reaction through a subclass B2-like mechanism, not the subclass B3 mechanism.Acta Crystallographica Section F: Structural Biology Communications 04/2014; 70(Pt 4):424-32. DOI:10.1107/S2053230X14003926
Article: Crystal Structure of Pedobacter heparinus Heparin Lyase Hep III with the Active Site in a Deep Cleft[Show abstract] [Hide abstract]
ABSTRACT: Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1-4 glycoside bonds between uronate and amino sugar residues. As different from Hep I and Hep II, Hep III is specific for heparan sulfate. Here we describe the crystal structure of Hep III with active site located in a deep cleft. The X-ray crystallographic structure of Hep III was determined at 2.20 Å resolution using single-wavelength anomalous diffraction. This enzyme comprised an N-terminal α/α-barrel domain and a C-terminal antiparallel β-sheet domain as its basic scaffold. Overall structures of Hep II and Hep III were similar, although Hep III exhibited an open form compared with the closed form of Hep II. Superimposition of Hep III and heparin tetrasaccharide-bound Hep II suggested that an active site of Hep III was located in the deep cleft at the interface between its two domains. Three mutants (N240A, Y294F, and H424A) with mutations at the active site had significantly reduced enzyme activity. This is the first report on the structure-function relationship of P. heparinus Hep III.Biochemistry 01/2014; 53(4). DOI:10.1021/bi4012463 · 3.02 Impact Factor
Article: Crystal Structure of a Bacterial Unsaturated Glucuronyl Hydrolase with Specificity for Heparin*[Show abstract] [Hide abstract]
ABSTRACT: Extracellular matrix molecules such as glycosaminoglycans (GAGs) are typical targets for some pathogenic bacteria, which allow adherence to host cells. Bacterial polysaccharide lyases depolymerize GAGs in β-elimination reactions, and the resulting unsaturated disaccharides are subsequently degraded to constituent monosaccharides by unsaturated glucuronyl hydrolases (UGLs). UGL substrates are classified as 1,3- and 1,4-types based on the glycoside bonds. Unsaturated chondroitin and heparin disaccharides are typical members of 1,3- and 1,4-types, respectively. Here we show the reaction modes of bacterial UGLs with unsaturated heparin disaccharides by X-ray crystallography, docking simulation, and site-directed mutagenesis. Although streptococcal and bacillus UGLs were active on unsaturated heparin disaccharides, those preferred 1,3- rather than 1,4-type substrates. The genome of GAG-degrading Pedobacter heparinus encodes 13 UGLs. Of these, Phep_2830 is known to be specific for unsaturated heparin disaccharides. The crystal structure of Phep_2830 was determined at 1.35 Å resolution. In comparison with structures of streptococcal and bacillus UGLs, a pocket-like structure and lid loop at subsite +1 are characteristic of Phep_2830. Docking simulations of Phep_2830 with unsaturated heparin disaccharides demonstrated that the direction of substrate pyranose rings differs from that in unsaturated chondroitin disaccharides. Acetyl groups of unsaturated heparin disaccharides are well accommodated in the pocket at subsite +1, and aromatic residues of the lid loop are required for stacking interactions with substrates. Thus, site-directed mutations of the pocket and lid loop led to significantly reduced enzyme activity, suggesting that the pocket-like structure and lid loop are involved in the recognition of 1,4-type substrates by UGLs.Journal of Biological Chemistry 01/2014; 289(8). DOI:10.1074/jbc.M113.522573 · 4.57 Impact Factor
Article: Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2[Show abstract] [Hide abstract]
ABSTRACT: Glycinin is one of the most abundant storage-protein molecules in soybean seeds and is composed of five subunits (A1aB1b, A1bB2, A2B1a, A3B4 and A5A4B3). A1bB2 was purified from a mutant soybean cultivar containing glycinin composed of only A5A4B3 and A1bB2. At 281 K the protein formed hexagonal, rectangular and rod-shaped crystals in the first [0.1 M imidazole pH 8.0, 0.2 M MgCl2, 35%(v/v) MPD], second [0.1 M sodium citrate pH 5.6, 0.2 M ammonium acetate, 30%(v/v) MPD] and third (0.1 M phosphate-citrate pH 4.2, 2.0 M ammonium sulfate) crystallization conditions, respectively. X-ray diffraction data were collected to resolutions of 1.85, 1.85 and 2.5 Å from crystals of the three different shapes. The crystals belonged to space groups P6322, P21 and P1, with unit-cell parameters a = b = 143.60, c = 84.54 Å, a = 114.54, b = 105.82, c = 116.67 Å, β = 94.99° and a = 94.45, b = 94.96, c = 100.66 Å, α = 107.02, β = 108.44, γ = 110.71°, respectively. One, six and six subunits of A1bB2 were estimated to be present in the respective asymmetric units. The three-dimensional structure of the A1bB2 hexamer is currently being determined.Acta Crystallographica Section F Structural Biology and Crystallization Communications 08/2013; 69(Pt 8):937-941. DOI:10.1107/S1744309113019684 · 0.53 Impact Factor
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ABSTRACT: Pyridoxine 4-oxidase (PNOX) from Mesorhizobium loti is a monomeric glucose-methanol-choline (GMC) oxidoreductase family enzyme, catalyzes FAD-dependent oxidation of pyridoxine (PN) into pyridoxal, and is the first enzyme in pathway I for degradation of PN. The tertiary structures of PNOX with a C-terminal His6-tag and PNOX-pyridoxamine (PM) complex were determined at 2.2Å and at 2.1Å resolutions, respectively. The overall structure consisted of FAD-binding and substrate-binding domains. In the active site, His460, His462, and Pro504 were located on the re-face of the isoalloxazine ring of FAD. PM binds to the active site through several hydrogen bonds. The side chains of His462 and His460 are located at 2.7 and 3.1Å from the N4' atom of PM. The activities of His460Ala and His462Ala mutant PNOXs were very low, and 460Ala/His462Ala double mutant PNOX exhibited no activity. His462 may act as a general base for abstraction of a proton from the 4'-hydroxyl of PN. His460 may play a role in the binding and positioning of PN. The C4' atom in PM is located at 3.2Å, and the hydride ion from the C4' atom may be transferred to the N5 atom of the isoalloxazine ring. The comparison of active site residues in GMC oxidoreductase shows that Pro504 in PNOX corresponds to Asn or His of the conserved His-Asn or His-His pair in other GMC oxidoreductases. The function of the novel proline residue was discussed.Biochimica et Biophysica Acta 03/2013; 1834(6). DOI:10.1016/j.bbapap.2013.03.004 · 4.66 Impact Factor
Article: Crystal structure of a major seed storage protein, 11S proglobulin, from Amaranthus hypochondriacus: Insight into its physico-chemical properties[Show abstract] [Hide abstract]
ABSTRACT: Amaranth is a crop known for its high quality proteins. 11S Globulin is one of the most abundant and important storage proteins of the amaranth grain. Here, we report the crystal structure of amaranth 11S proglobulin at a final resolution of 2.28 Å. It belonged to the space group P6(3) with cell dimensions a=b=96.6, c=75.0 Å. It contains one asymmetric unit consisting of 372 residues and 100 water molecules. Disordered regions in the model approximately correspond to the variable regions of the 11S globulins. The structure has an extended α-helix and β-barrel domains at both N-terminal and C-terminal regions, which are characteristic of the 11S and 7S globulins. The three dimensional structure suggests that its high thermal stability is due to the cumulative effects of many factors and its good emulsifying property depended on the balance between its surface hydrophobicity and hydrophilicity.Food Chemistry 11/2012; 135(2):819-26. DOI:10.1016/j.foodchem.2012.04.135 · 3.39 Impact Factor
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ABSTRACT: β-1,4-Mannanase (EC 188.8.131.52) catalyzes the hydrolysis of β-1,4-glycosidic bonds within mannan, a major constituent group of the hemicelluloses. Bivalves and gastropods possess β-1,4-mannanase and may degrade mannan in seaweed and/or phytoplankton to obtain carbon and energy using the secreted enzymes in their digestive systems. In the present study, the crystal structure of AkMan, a gastropod β-1,4-mannanase prepared from the common sea hare Aplysia kurodai, was determined at 1.05 Å resolution. This is the first report of the three-dimensional structure of a gastropod β-1,4-mannanase. The structure was compared with bivalve β-1,4-mannanase and the roles of residues in the catalytic cleft were investigated. No obvious binding residue was found in subsite +1 and the substrate-binding site was exposed to the molecular surface, which may account for the enzymatic properties of mannanases that can digest complex substrates such as glucomannan and branched mannan.Acta Crystallographica Section F Structural Biology and Crystallization Communications 10/2012; 68(Pt 10):1164-8. DOI:10.1107/S1744309112037074 · 0.53 Impact Factor
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ABSTRACT: The structures of two mutants (H192A and Y246F) of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate [4-deoxy-L-erythro-hex-4-ene-pyranosyluronate-(mannuronate)(2)-mannuronic acid] were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form of the H192A mutant. The final models of the complex forms, which comprised two monomers (of 353 amino-acid residues each), 268-287 water molecules and two tetrasaccharide substrates, had R factors of around 0.17. A large conformational change occurred in the position of the lid loop (residues 64-85) in holo H192A and Y246F compared with that in apo H192A. The lid loop migrated about 14 Å from an open form to a closed form to interact with the bound tetrasaccharide and a catalytic residue. The tetrasaccharide was bound in the active cleft at subsites -3 to +1 as a substrate form in which the glycosidic linkage to be cleaved existed between subsites -1 and +1. In particular, the O(η) atom of Tyr68 in the closed lid loop forms a hydrogen bond to the side chain of a presumed catalytic residue, O(η) of Tyr246, which acts both as an acid and a base catalyst in a syn mechanism.Acta Crystallographica Section D Biological Crystallography 09/2012; 68(Pt 9):1207-16. DOI:10.1107/S090744491202495X · 2.67 Impact Factor
Article: Crystal structure of enzyme–substrate complex of protein-glutaminase obtained by the mutant of pro-enzymeActa Crystallographica Section A Foundations of Crystallography 08/2012; 68(a1):s168-s168. DOI:10.1107/S0108767312096766 · 2.31 Impact Factor
Article: The extension peptide of plant ferritin from sea lettuce contributes to shell stability and surface hydrophobicity[Show abstract] [Hide abstract]
ABSTRACT: Plant ferritins have some unique structural and functional features. Most of these features can be related to the plant-specific "extension peptide" (EP), which exists in the N-terminus of the mature region of a plant ferritin. Recent crystallographic analysis of a plant ferritin revealed the structure of the EP, however, two points remain unclear: (i) whether the structures of well-conserved EP of plant ferritins are common in all plants, and (ii) whether the EP truly contributes to the shell stability of the plant ferritin oligomer. To clarify these matters, we have cloned a green-plant-type ferritin cDNA from a green alga, Ulva pertusa, and investigated its crystal structure. Ulva pertusa ferritin (UpFER) has a plant-ferritin-specific extension peptide composed of 28 amino acid residues. In the crystal structure of UpFER, the EP lay on and interacted with the neighboring threefold symmetry-related subunit. The amino acid residues involved in the interaction were very highly conserved among plant ferritins. The EPs masked the hydrophobic pockets on the ferritin shell surface by lying on them, and this made the ferritin oligomer more hydrophilic. Furthermore, differential scanning calorimetric analysis of the native and its EP-deletion mutant suggested that the EP contributed to the thermal stability of the plant ferritin shell. Thus, the shell stability and surface hydrophobicity of plant ferritin were controlled by the presence or absence of the plant-ferritin-specific EP. This regulation can account for those processes such as shell stability, degradation, and association of plant ferritin, which are significantly related to iron utilization in plants.Protein Science 06/2012; 21(6):786-96. DOI:10.1002/pro.2061 · 2.85 Impact Factor
Article: Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris[Show abstract] [Hide abstract]
ABSTRACT: The medaka fish α-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant α-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enzyme activity. The purified protein was also crystallized and examined by X-ray crystallography. The structure has the (α/β)(8) barrel fold, as do other known α-amylases, and the overall structure is very similar to the structure of vertebrate (human and pig) α-amylases. A novel expression plasmid was developed. Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein.Biochimica et Biophysica Acta 05/2012; 1824(8):954-62. DOI:10.1016/j.bbapap.2012.05.005 · 4.66 Impact Factor
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Seoul, Seoul, South Korea
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All posts by Rosemary Kent
King of the Catalogs
Who is Roger Horchow and why is he doing these terrible things to our Christmas budgets?
Hello, Walls/ Design
When is a wall not a wall? When itÌs a work of art.
Out of the Closet
Texas fashion is coming into its own, but no one yet has lost money by underestimating Texas taste.
Texas fashion is not exactly a rags-to-riches story.
A Homesick Texan's Guide to New York
There are times, no matter where you are, when you must have a nacho.
The Bronx is up and the Battery's down and there are little bits of Texas all over the place.


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|Reviews for We Believe
| Flotsam And Jetsam chapter 4 . 3/8
Great story
| hatondog chapter 4 . 6/26/2014
Bravo. I've seen a number of fics tackle the post TRF period and yours is certainly among the most entertaining and satisfying. I love the articulate proSherlock reporter turned Editor, whose voice is like a ray of hope through the slime spread by others. I'm not in any way a Johnlocker, but you touched on that direction in a way that fit OK with the story. Well done.
| Once Upon a Faerytale chapter 4 . 1/17/2014
Love it, love it, love it.
| Ralina chapter 4 . 1/3/2014
I really liked the fanfic and I am glad there is a sequel. Can't wait to read it.
| Me chapter 4 . 11/26/2013
That was amazing. One of the best post-Reichenbach tics I've read. :D going straight to the sequel now
| rana2001 chapter 4 . 11/25/2013
Wow. WOOW.
At that bit before the last newspaper thing I started giggling and looked at my cat and she looked back weirdly and I pointed to the screen and went back to reading.
| Equality4Puppies chapter 4 . 9/21/2013
This is absolutely wonderful.
| FunkyColdMedina chapter 4 . 8/20/2013
That was just brilliant
| sammatha chapter 1 . 8/11/2013
This is one of the most beautiful stories i have ever read! I will read it over and over, you've captured all the Sherlock characters personalities perfectly. XD Your an amazing writer! Keep up the good work!
| NicoleCollard chapter 4 . 7/21/2013
Wonderfil story. I love the way you've brought all these important characters together in the plot. We'll always believe in Sherlock Holmes! :)
| autumnfierystar04 chapter 1 . 7/6/2013
OMG, have been looking for a story about the #ibelieveinsherlock movement for ages, thank you for such a wonderful first chapter!
| ResidentBunburyist chapter 1 . 7/4/2013
One of my favorite Post-TRF stories out there. You capture John so beautifully.
| Rudolphsd chapter 4 . 6/24/2013
Words cannot describe how amazing this fanfiction is. EVERY OTHER BLOODY JOHNLOCK FANFIC IS SO BAD! It's so lemony I want to throw up, with good plots but marred by the INSANE LEMON-NESS! You are the light at the end of the fanfiction tunnel. I was about to give up when I read this. IT. IS. AMAZING. Gahhh, I love you for writing this!
Thanks again
| Proper the Incredible chapter 4 . 5/4/2013
This fic was AWESOME!
| Riya Morut chapter 3 . 4/15/2013
You know, I don't like this "(Continued on page X)" nonsense. Cutting me off in the middle of a sentence is like ending a song on a half cadence. It just isn't done!
Other than than, I like where most of this is going. I'm not a Johnlock shipper (Mary Morstan is canon, and shipping the two of them always seems to imply that one can't have a good relationship unless it's based on romantic attachment) but I can overlook that for the plot. And really, you've done a fantastic job of turning this from one sentence into a huge movement. Kudos!


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JoneSoft UnInstall Cleaner
editor's review
JoneSoft UnInstall Cleaner is an application that can remove installed programs from your computer. It intends to replace the "Add/Remove Programs" function built into Windows.
This is a portable product, so installing JoneSoft UnInstall Cleaner is not required. In this case, you can store the tool on an external device (like a USB flash drive), plug it into any computer and directly run its executable file. More importantly, there will be no modifications made to your Windows registry items.
The interface of JoneSoft UnInstall Cleaner is plain and simple to work with. A list of all installed applications is automatically displayed upon initialization. You can check out a detailed description on each item when you select it.
Besides removing a program, you can also modify its values (display name and uninstall command), as well as add a new registry entry by inputting the display name, uninstall command and registry entry name.
The simplistic tool uses a very low amount of system resources (which can be barely noticed), has a good response time and didn't freeze, crash or pop up errors during our tests. On the other hand, there is no help file available, the interface is outdated and you cannot even use a search function. All in all, JoneSoft UnInstall Cleaner lacks many essential features which are required for an applications uninstaller. We recommend you look for something more advanced than this tool.
top FREE alternatives


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Photography:Phillip Glickman
Floral Design:Southern Floral
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Location:Austin, Texas, North America, United States
Dining under the stars. It's one of my favorite ways to celebrate saying "I do". And dining under the stars with a decidedly vintage vibe and layers upon layers of gorgeous blooms from Southern Floral? Even better. Which is why this sweet Austin affair plannedRead More On SMP


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Calmar Stadshotell
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Travel Blogs from Kalmar
Kalmar. Sweden
... and with a very strong tail wind. We ate in town harbour in the warm sun; fish of course, although John was denied a Wallenberg (fish cake) as the last one had just sold to the people just before him! After lunch we drove on through the amazing Swedish countryside, wheat and barley fields swirling to the horizon, huge farmsteads and barns, even a windmill. Unfortunately the canola wasn't quite ripe, but you can't have everything. But we did see a field ...
Heading South
... an ocean pool, a fairly impressive diving board and several sauna huts. No one was about though, summer is over and there is not a soul around. We started this gap year journey because we had enjoyed annual ski holidays in the northern hemisphere for so many years, and often wondered what the places we visited would be like in summer, and here we are in Scandinavia in September and summer is already over, imagine only having 2 months of summer a year!
The Kronan
... lost their lives and Sweden was now minus her largest warship and incidentally, the Admiral of the Realm and commander of the Swedish navy, Lorenz Creutz. Actually, things would get even worse as her second largest warship, the Sv�rdet under the command of the suddenly elevated replacement overall commander Admiral Claes Uggla, suffered a similar fate, as did he, poor chap and another 650 men died that day. All in all, such a bad result that sometime later, a special commission ...
And then there were Two
... than a return visit to the glassblower, who was there now and busy making some simple pieces, we may come back again when he is doing something more complex. That and a visit on board our neighbour' s boat – they came in late yesterday having motored for 15 hours from V�stervik. It was 'Freya of Mersey', belonging to Tony and Louise Halliwell. They keep Freya in Fehmarn too and we last spent time with them during the 2012 Rally in ...
Day in Harbour
... plug to a UK one! I was deeply impressed.
We then visited the museum, at least we got as far as the entrance but we discovered that it closed within an hour and unlike the art museum, I reckon that there would be at least an hour's worth of things to see, so the decision was made that Julie and I would call back over the coming wind-bound days and that the remainder of the afternoon would be spent mooching about the shops (not my idea but ...