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Loose Lips Quotes of 2009: Vincent Gray
"Maybe we ought to just disband this council. Why did we spend hours working on a budget...only for you to second-guess us?"
—D.C. Council Chairman Vincent C. Gray, Oct. 29
Michelle Rhee's make-nice tactics ended on Sept. 16, when she announced that teachers would be laid off for the first time in years. When 229 of them got the boot on Oct. 2—at least some of them assisted by police—local legislators went nuts, none more than Gray. Exacerbating the conflict was Rhee's insistence that the cuts were due to mid-summer budget cuts by the council; Gray insisted that he and his colleagues had cut only summer school, not teachers. What came out at an Oct. 29 hearing was that the school system's budget woes were worse than the council knew: Millions in overspending were kept in the dark, and Rhee decided to cut teachers rather than summer school. And At-Large Councilmember Michael A. Brown became the first legislator to publicly suggest that Mayor Adrian M. Fenty's control of the school system be revoked. "It's time to think about reforming the reform," he said on the dais.
Photo by Darrow Montgomery

subset: commoncrawl


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IDAHO FALLS — Rex Rammell has had a change of heart regarding who is invited to his "elders of Israel only" meetings.
At a press conference Thursday, the Idaho gubernatorial candidate issued a formal apology with regards to his exclusion of non-Mormons from his upcoming meetings dealing with the so-called "White Horse Prophecy."
Reading from a prepared statement, Rammell said he was wrong to assume that nonmembers of The Church of Jesus Christ of Latter-day Saints wouldn't be interested in hearing his message.
"To all those citizens who are not members of the LDS faith, who have expressed a sincere interest in attending my meetings and discussing this prophecy and how we can step forward and save the United States Constitution, I would like to apologize and invite them wholeheartedly to join us," said Rammell.
Rammell also says that he hopes that the meetings will bring people of all faiths together to "unite in a common cause … to save the Constitution."
"The theme of my campaign has been, will be, and as governor, will always be states' rights," Rammell told reporters. "And so, you don't have to be LDS to contribute to that discussion, and I think they will contribute greatly."
The LDS Church has issued a statement distancing itself from Rammell. The church also does not consider the prophecy, which is said to have come through its founder, Joseph Smith, as doctrine.
Although Rammell's controversial meetings have made regional and state-wide news for several weeks, earlier this week, the controversy was picked up by various national media organizations, including the DrudgeReport.
Rammell's first meeting will be on Jan. 19, at the Hampton Inn in Idaho Falls at 7 p.m. A subsequent meeting will be held in Rexburg on Jan. 21, at the Marriott Hotel at 7 p.m.
The events are free and open to everyone.
For more information about Rex Rammell or his campaign, visit www.voterammell.com.


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New Mont Blanc La Vie Boheme 101731 Ladies Leather Wallet - Mother's Day Special For SaleMontblanc
Ref No. 101731 - Lades Compact Wallet
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23-year-old pseudo-adult, grad student, writer, and word search enthusiast.
Forever devastated over the loss of inkpop though I will eventually settle in here. Will also eventually learn to make better looking covers. Be patient, my friends.
Location:Cleveland, Ohio
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6 Published Works
From an Empty Bed
Description: Subpar, 3 AM poetry penned under the influence of Jack Daniels (and a few less awesome men). *Note: There is a fair amount of quoted material throughout this collection. Some are idioms, most are song lyrics. There are also a few allusions to songs...
Other Works by ElyssaAllen.
Carolina Summer
Fresh off the heels of his sophomore year at the University of Baltimore, Tanner Coyne is not thrilled a...
Meet Me at the Lighthouse
The first flash fiction piece I wrote for my creative writing class. The assignment was to write a stor...
A segmented essay I wrote for Creative Nonfiction Workshop, broken into the months spent with a boy who...
Letters to You
I fell in love at seventeen and it was terribly inconvenient. Parts of these letters have made their wa...


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Are you going to let your car define where you go or let your driving needs define the car you pick?
Your thought that "With many of the square formatists I look at however, their chosen frame format defines their aesthetic" is in my opinion backward. I think that their chosen aesthetic drove their frame format choice. I.e. they bought the car that fit their driving needs.
I would also suggest that there are many photographers who use a variety of formats successfully. I know some of these guys personally. One, as far as I know only has two cameras, an 8x10 and an iPhone. Another does digital at his day job and 4x5 for all his personal stuff. For both the work they do with each camera is very different, but both sets of work for each are great. For both their camera choices are defined by the result they want in a given setting.


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It's time to be a #HarryPotterSpotter! From now through November 2, Bloomsbury Publishing is sponsoring a Harry Potter artwork scavenger hunt. Twelve prints of Jim Kay's illustrations for Harry Potter and the Philosopher's Stone are currently on display in twelve art museums across Britain, and fans are invited to find them all and document their search on social media.
The hunt is in collaboration with Kids in Museums and The Telegraph. Clues to the locations of the prints within the museums in the UK and Ireland are on the Bloomsbury website. Each print is on display in a free museum or in free areas of museums. Only one print needs to be found to enter the contest, but participants are welcome to search for all twelve.
The overall winner of our Harry Potter Spotter competition will receive family tickets to the Warner Bros. Studio Tour London, a Jim Kay signed print of their choice and a copy of the Deluxe Illustrated Edition of Harry Potter and the Philosopher's Stone. Twelve additional prize winners will receive a Harry Potter tote bag, a Jim Kay signed print (of the print they spotted) and a copy of Harry Potter and the Philosopher's Stone Illustrated Edition.
To play, simply take a photograph of yourself in front of one of the prints on display in a museum. (If you don't want to be in the photo, you can just take a picture of the print.) Share your photo on Twitter or Instagram, using #HarryPotterSpotter. Images should be tagged to @KidsBloomsbury and the specific museum on Twitter, or @BloomsburyPublishing and the specific museum on Instagram. Don't forget to see more of the museum while you are there!
For a full slideshow of the artwork, participating museums, and clues, see here.Add a Comment


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- Northern New York Newspapers
- Watertown
- Ogdensburg
- Massena-Potsdam
- Lowville
- Carthage
- Malone
- NNY Business
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Paul Singer, a billionaire hedge fund manager a Republican donor, has contributed to a third political action committee supporting congressional candidate Elise M. Stefanik.
On May 14, Mr. Singer donated $100,000 to New York 2014, a PAC connected with John Faso, a 2006 gubernatorial candidate for New York state.
New York 2014 has spent $366,299 on direct mail and television advertising supporting Ms. Stefanik in the Republican primary election.
Mr. Singer also donated $250,000 to American Crossroads, a GOP super PAC running attack ads against Ms. Stefanik's opponent, Matthew A. Doheny, and is connected to Winning Women, a PAC that donated $110,000 to Ms. Stefanik's campaign.
The primary election is Tuesday. Ms. Stefanik has been endorsed by the New York state Conservative Party and Mr. Doheny has been endorsed by the state Independence Party.
Democrat Aaron G. Woolf and Green Party candidate Matthew J. Funiciello are also in the race.
Thousands of readers like you have made Northern New York Newspapers the number one ranked website* for local news and sports coverage in the north country.
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District: 651.351.8340 | Fax: 651.351.8380 | email1875 Greeley Street South, Stillwater, MN 55082
Program named to National Speech and Debate Association's prestigious 100 Club.
School board members are considering a proposal to add an elementary Spanish immersion program for the 2017 school year.
We want your feedback on the proposed 2016-2017 school calendar.
District Office: 1875 Greeley Street South, Stillwater, MN 55082 | Office: 651.351.8340 | Fax: 651.351.8380 | email us© 2015. Stillwater Area Public Schools Privacy Policy
Serving the communities of Afton, Bayport, Baytown Township, Grant, Hugo, Lake Elmo, Lakeland, Lakeland Shores, Lake St. Croix Beach, Marine on St. Croix, May Township, Oak Park Heights, St. Mary's Point, Stillwater, Stillwater Township, West Lakeland Township, Withrow, and Woodbury.


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Intercontinental Dusseldorf
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Airport Transportation
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Historical Traveler Reviews Intercontinental Dusseldorf Düsseldorf
Top Service
Wir sind gerade von einem wunderbaren Aufenthalt im Interconti in Düsseldorf zurückgekehrt. Wir waren bereits zweimal zuvor im Interconti in Köln und haben dieselbe Qualität in Düsseldorf erwartet. Wir wurden nicht enttäuscht! Alle, von der Rezeption bis hin zum Housekeeping waren sehr freundlich! Hier versteht man, dass es wirklich um den Gast geht. Die Inneneinrichtung ist anders, als jedes Hotel, in dem wir übernachtet haben, insbesondere die Lobby mit einem hohen Glasdach war wunderschön. Auch die Zimmer waren im Altbaustil mit hohen Wänden und wertvollen Möbeln. Das Frühstück war auch sehr gut und die Angestellten waren sehr hilfsbereit und freundlich. Es ist schade, dass man für Holmes Place (das Fitnesscenter) bezahlen muss, denn in der Vergangenheit war die Nutzung kostenlos. Ein Tipp: Wenn Sie, so wie wir, ein Ambassador Mitglied der Interconti Gruppe sind, dann erhalten Sie einige Privilegien. Überprüfen Sie die Webseite für weitere Informationen.
We were friends traveling for fun! We stayed four nights booked online special deal. The place is perfect for all sorts of trips. Facilities are modern and optimized for customers. The lobby is inviting. The staff is so friendly and helpful, from the porters, concierge, to the restaurant staff.
The hotspot feature is excellent, however the facilities in the business center are not as good, with internet speed so slow and the printer was out of order throughout our stay!
The rooms are excellent with all amenities. Cleaning was delayed when
returning after 5 hours out to an undone room. The bathroom was not always cleaned to the anticipated level expected from such hotel.
In general, an excellent hotel and experience. Needs a bit more of quality assurance on house keeping.
We stayed here twice last December. Both times it was perfect. They helped us verry well, the room was gorgeous and breakfast was perfect. Besides that the location is top.
We will definitly return to this hotel.
Top service
Just returned from a wonderfull stay at the intercontinental in Dusseldorf. We've also been a couple of times to the intercontinental in Koln so we expected the same qualitylevel in Dusseldorf. They didn't disapoint us! Everybody, from frontdesk to housekeeping, was very friendly. They really understand it's all about peoples business. The interior is different then any other hotel we stayed in. Espacially the lobby with the high roof of glass was very beautifull. Also the room was high end quality furniture. The breakfast was also very good and also the staff was very helpfull and friendly. It is a pitty that you have to pay for holmes place (fitnessclub) because it was for free in the past. tip: If you are an ambassador member of the intercontinental like us you will receive some privileges! check there website for more information. A.Mateman, Netherlands
A lot of money for a poor experience
When you pay top dollar you expect the best. I did, and didn't get it. For more than EUR400 per night (yes, you read that correctly) I received a small room, with a peculiar absence of drawers (a common feature in Germany, it seems). Plus a foul stench emanating from the bathroom that someone had to attend to fix. My experience (and mood, given the comparative sums we had paid) was not helped either by the presence of a vast group of visiting Mexican football fans, who effectively rendered facilities like the buffet breakfast unusable - the hotel just couldn't cope with the number of simultaneous arrivals for meals, etc. My point, then, is that they either shouldn't have taken their booking in the first place or should have offered everyone else in the hotel a suitable discount by way of compensation. Instead, it seems, I paid a hefty premium. From a PR perspective, this was a disaster for InterCon and they should have been a great deal more intelligent.
On the bright side, the location is good - assuming, that is, that anything in Dusseldorf qualifies for such a description.
Best in Dusseldorf, but restaurant not perfect yet.
The brand new Intercontinental is a great hotel. Some minor teething problems like missing room directions and a not perfect check-in (compensated by a nice upgrade), but nothing too bad. Very well designed, great quiet rooms, perfect location (quiet part of the Ko) and wonderfull beds. Bathroom was also very nice.
Very nice doormen and other staff.
Breakfast was excellent and service very well.
Also had diner here one night. Not bad, but to be honest not good enough for the price.
Next time I'm in Dusseldorf I'm staying here.
Fantastic hotel, fab location
Stayed here for 3 nights over Xmas, and this hotel was everything we hoped for and more. The staff were really very helpful (thanks Stefan), and the service, ambience, feel and warmth of the hotels staff shone through this excellent, up market hotel.
Would LOVE to go back one day, when I'm rich!
Very classy hotel but not perfect
Stayed here in March 2006 on business and was immediately impressed with the design, very simple and very elegant - in fact very Intercontinental!
Lobby was grand, very well designed with excellent atrium
Room is very european, dark wood and beige interiors.
Very comfatable furnishings and veryyyyyyyyyyy good bed BUT NO FLAT SCREEN TV, kinda dissapointed for a newly built European five star hotel.
Bathroom was excellent, separate shower and big bathtub.
Room service was quick and efficient, brought exactly what I ordered (sirloin and roast potatoes) within 15 mins of putting down the phone, food was very yummy!
Club lounge was very good, good selection of foods and drink but it only has two PC's with internet access and was surprisingly empty.......very comfatable lounge with excellent reading materials though!
Breakfast was your typical European stuff, Sausages, bacon, eggs, pasteries, breads, juices, yougert, fruit cerael etc.......very good quality and plenty of it!
Check in and check out was fast and efficient! Staff are very polite.......However......
Took two calls to get a DVD player installed, not really good enough for a five star service
Also, emailed them to say I left my belt in the hotel room and they charged me extra for breakfast when this was included in the room rate.........still waiting a response from the hotel....bad after sales service.
But all in all, a very impressive modern five star joint, just a few rough edges though which robs it of a five star mark in my opion.
The first to stay in our room!
We were here 1 week after they opened and even received a welcome cake in our room as we were the first to have stayed in it! The hotel is very airy/modern and full of windows and natural lighting. The rooms are a decent size (as are the modern bathrooms). The bed was very comfortable, my only complaint would be that you were only given a feather comforter. It was luxurious, but with it on I was too hot, and with it off, too cold, so I kept waking up through the night. Another option would have been nice. Other than that, absolutely no complaints! Great location off the Ko, and a couple block walk to the Altstadt. Only a block to the U-Bahn (subway). Would definitely stay here again!
New 5-Star Hotel Downtown
This hotel just opened at the KÖ in Düsseldorf.
All shops are in walking distance.
The rooms are very nice and spacios.
I recommond this hotel if you stay in Düsseldorf.
Very good breakfast.
A minus as you have to pay extra for fitness/sauna/ pool.
TripAdvisor Reviews Intercontinental Dusseldorf Düsseldorf
Travel Blogs from Düsseldorf
Getting' ready to cruise
... never to go on that kind of tour, no matter how decrepit we become. We have enough independence in us to resist such a tour. It sounded ghastly. Also bought some more Loratadine hay fever tablets. We've discovered they sell 100 hay fever tabs here for $15 Aus. I pay four times that in Melbourne, so who is making all the profit? We met some people at the hotel also headed for the Richard Rohr cruise. They were catching a taxi, but we decided to train it, which we ...
Fun day in Cologne
... way around the malls. Reminded me of medieval troubadours and didn't seem to be asking for money. Just doing it for the love of making people happy. We succeeded in getting ourselves hopelessly lost. We thought we were going in the right direction but discovered we weren't when a nice German man asked if we needed help. He showed us that we were actually heeded in the opposite direction to our hotel. We thought we were going towards it. Rosanne suggested it would ...
Super-sized barges
... in we head over the bridge covered in padlocks to visit the cathedral. It must have been one of the few buildings to remain after Cologne was heavily bombed.
Lovely city..so we hopped on the inevitable tourist bus which zipped us round the sights...
Dinner at another beer hall...very different from the others. Beer is served in a 0.2l ...
... that was outside the city. From what we gathered it was a steel and/or energy production plant. It was interesting to see how large the machines and structures stood. It also offered quite a view. There was also a little food stand where I trie my very first bratwurst. It was actually quite tasty with some mustard on it. The girls then proceeded to take me to a place called magic mountain. ...
School and Chinafest
... mornings, school has gone well this week, and I even took a whole 45-minute class on my own! The teacher told me 2 minutes before the end of break that he had to do something urgent, and that I should read them a story, check comprehension and go through some true/false questions with them, and so I did that, and had lots of time left so also made them search for Names, Adjectives and Verbs. I think I did an alright job, especially ...


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PAUL'S BLOG (Click here to see past guest blogs).
The Weight: A guest blog by Cassie J Sneider
I discovered that I could make a butt with my stomach when I was in the third grade, catapulting my belly up the list of favorite body parts, right up there with my armpits and any area that could generate a fart sound.
"Mom, check it out! A BUTT!" I would say, squeezing together the fat around my belly button and dancing a little, sometimes a shoulder shimmy, sometimes a rolling motion like a sexy extra in the director's cut of Aladdin. My younger sister would try to mimic me, but she took after my mother's side, small-framed, skinny ... (Read the Rest of This Post)
(Read the Rest of This Post)
What to Expect When You're Expecting to Die: Guest Blog by Glenn Rockowitz
I don't cry anymore. And I hate that I don't. I have always attributed this weird fucking paralysis to the emotional lockdown I developed years ago when I was diagnosed with cancer and given only a few months to live. A survival mechanism I designed to keep my eyes fixated only on reaching the living Me at the other side of the brushfire. And it has served me well. Or so I have told myself.
This morning I cried in a way I haven't since I was kid. The guttural kind of sobbing that only comes from a body purging ... (Read the Rest of This Post)
(Read the Rest of This Post)
Navigating Higher Education with a Disability: Guest Blog by Dr. Shirag Shemmassian
How I Navigated Higher Education with a Disability and How You Can Help Your Child Do the Same
Dr. Shirag Shemmassian
Attending Armenian schools in Los Angeles throughout my childhood was rough. Sure, I was distractible in class and sometimes spoke out of turn, but that's not why peers and teachers kept their eyes on me. That attention came from my facial and vocal tics, which led to a Tourette Syndrome (TS) diagnosis around age 9 that I carry to this day.
TS is interesting because tics are so obvious; you can manage them for so long before people literally ... (Read the Rest of This Post)
(Read the Rest of This Post)
Rewiring the Brain After Trauma: A Guest Blog by Rachel Grant
It's Nothing But a Neuron! Exploring How to Re-train the Brain and Heal from Sexual Abuse
Have you ever walked by a pie shop and, upon smelling a fresh backed pumpkin pie, been transported back in time to a fond memory of Thanksgiving? Or maybe caught a glimpse of a stranger with certain features and found yourself thinking about that girl or guy from way back when? How about a significant other who one day playfully wrestles with you, and all of a sudden you find yourself lashing out at him without really understanding why? What exactly is occurring neurologically ... (Read the Rest of This Post)
(Read the Rest of This Post)
Cutting Contact With Her Mom: An email from Barbara in NC
Hi Paul-
I have tried to start this letter to you so many times (and here I go again!), but listening to what you said about missing your mom on a recent show finally got me to do it. I don't think anyone that has never experienced the point of getting to where you have to cut ties with a parent for your own survival could ever understand.
Since I don't want this to turn into a long, drawn out story, I will hit the highlights:
- Mother was raised Irish Catholic, so guilt and shame and not talking about feelings
(Read the Rest of This Post)


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I'm building a website, and I'm using IE9 to test it (because I have to :( ). Recently I found out that IE is running on compatibility mode for IE7 which is ruining some of my layout.
To fix this I'm using the
<meta http-equiv="X-UA-Compatible" content="IE=edge"> tag.
So my question is, how do other browsers (Chrome, Firefox, etc...) behave upon this tag?


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Denny'sJune 8, 2014American • Orlando, United States
Ask for Charneise!!!!!
Bar service kinda sucks. Jim is okay but Gigi was not :0(
Ask for Wolverine (Chris) at the inside bar or Trinidad (Junior) if you're at the outside bar. Both are amazing & funny :0)
It was so much fun!!!! The captain & crew were very nice. Tip them well, they do an awesome job!!!!!
The hot sauce is HOT!! It's no joke!
If you're at the bar, ask for Tracy. She is AMAZING!!!!!
Hurry in and try the Gingerbread Martini!
The fish n' chips is spectacular!!! Well worth $6.95
Everyone here is always super nice. I love shopping here :o)
The seats here are AWESOME!! Stadium seating rocks XD


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I take the time to stop and smell the flowers. I enjoy time and find creative ways to spend it.
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27 • Portland, ME • Man
I'm looking for
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My details
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- 5' 9" (1.75m)
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- Buddhism but it's not important
- Sign
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text:
|Publication number
|Publication type
|Application number
|Publication date
|Mar 3, 1981
|Filing date
|Aug 21, 1979
|Priority date
|Sep 8, 1976
|Also published as
|Publication number
|Hans Bohn, Wilhelm Winckler
|Original Assignee
|Behringwerke Aktiengesellschaft
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (8), Non-Patent Citations (8), Referenced by (14), Classifications (22), Legal Events (1)
|External Links: USPTO, USPTO Assignment, Espacenet
______________________________________ Variation Mol % coefficient (%)______________________________________lysine 5,64 12.7histidine 1.05 15.0arginine 3.83 9.2aspartic acid 9.84 6.8threonine 4.33 15.7serine 4.78 8.2glutamic acid 11.29 3.5proline 6.41 9.2glycine 8.65 9.8alanine 7.51 6.4cystine/2 2.11 12.6valine 6.68 7.9methionine 0.98 14.5isoleucine 3.32 7.4leucine 14.43 4.2tyrosine 5.10 14.9phenylalanine 3.08 13.5and tryptophan 1.14 11.4______________________________________
This is a continuation-in-part-application of our application Ser. No. 830,775 filed Sept. 6, 1977, now abandoned.
This invention relates to a tissue specific protein, i.e. a substance which is a constituent of parenchymatous tissues, and to a process for preparing the same.
Several, already described, substances can be isolated in a relatively pure form from disintegrated organs of mammals. There is known, for example, the iron-containing protein ferritin, which can be prepared from placental tissue and which has been detected also in the stomach, spleen, liver, kidney, uterus and lung.
It is the object of the present invention to provide a novel tissue protein which is characterized by a protein proportion of 94Ý3%, essentially consisting of
α-amino acids,
an amino acid analysis of
______________________________________ Variation Mol % coefficient (%)______________________________________lysine 5,64 12.7histidine 1.05 15.0arginine 3.83 9.2aspartic acid 9.84 6.8threonine 4.33 15.7serine 4.78 8.2glutamic acid 11.29 3.5proline 6.41 9.2glycine 8.65 9.8alanine 7.51 6.4cystine/2 2.11 12.6valine 6.68 7.9methionine 0.98 14.5isoleucine 3.32 7.4leucine 14.43 4.2tyrosine 5.10 14.9phenylalanine 3.08 13.5and tryptophan 1.14 11.4______________________________________
a carbohydrate proportion of 5.4Ý2.2% consisting of 3.0Ý1% of hexoses, 1.2Ý0.5% of hexosamine, 0.2Ý0.2% of fucose and, 1.0Ý0.5% of neuraminic acid,
a sedimentation coefficient S20,wc=0.1 of 3.5Ý0.5 S,
a molecular weight determined by the sedimentation equilibrium method of 37,100Ý1,400,
an isoelectric point of 4.9Ý0.3, measured with polyamide gel plates and of 4.5Ý0.3, measured in an electrofocussing column, an electrophoretic mobility in the range between the α2 - and β1 -globulins,
an extinction coefficient E1 cm1% (278 nm) of 11.9Ý1.0, and a specific immunologic reaction with an antibody specifically directed against the protein.
It is a special characteristic of the novel tissue protein that it can be detected in fetal organs as well as in adult organs of mammals. In primates, and especially in men, the protein has been identified in the following fetal organs: heart, liver, kidney, lung, stomach, cerebrum, and in the following adult organs; heart, lung, skin, stomach, kidney, uterus, liver, spleen, suprarenal gland, colon, rectum, and bladder. In general, at least 10 mg of the protein can be detected in 100 g of tissue by quantitative immunologic methods. The product is also contained in the placenta in an amount of 10 mg per 100 g of tissue. For the isolation of the protein, the placenta is especially suitable. In erythrocytes 1 mg of the protein can be detected in 100 ml of erythrocytes, whereas in the plasma or serum of healthy individuals the protein cannot be detected.
To define the characteristic features of the protein the following methods were used:
The sedimentation coefficient was determined in an analytic ultra-centrifuge at 60,000 revolutions per minute in double sector cells with the aid of the migrating bands of the substance in the ultraviolet range at a wave length of 280 nm using water as solvent and a protein concentration of 1 mg/ml in a layering cell of an analytical ultra-centrifuge analogous to the method described by Vinograd inProc. Acad. Sci. USA, 49, 902 (1963)
The molecular weight was calculated, on the one hand, by determining the sedimentation equilibrium according to Yphantis. A value of 37,100Ý1,400 was found.
On the other hand, by analyzing the protein in a carrier gel consisting of polyacryl amide and containing 0.1% of sodium dodecyl sulfate, the major proportion of the protein was decomposed into 2 sub-units having a molecular weight of 22,000Ý2,000, from which a molecular weight of 44,000Ý4,000 could be derived for the native protein.
To determine the isoelectric point, the process of isoelectric focussing was used in the apparatus and with the reagents sold by Messrs. LKB, Stockholm, Sweden.
Using polyamide gel plates of Messrs. LKB, an isoelectric point of 4.9Ý0.3 was found, whereas electro-focussing in a column of the same firm with the recommended reagents indicated an isoelectric point of 4.5Ý0.3 for the protein of the invention.
The electrophoretic mobility of the protein was determined with the use of cellulose acetate as a carrier film.
The carbohydrates were determined according to H. E. Schultze, R. Schmidtberger, H. Haupt "Untersuchungen uber die gebundenen Kohlenhydrate in isolierten Plasmaproteinen" Biochem. Z., 329, page 490 (1958).
The amino acid analysis was carried out according to S. Moore, D. H. Spackman, W. H. Stein "Chromatography of amino acids on sulfonated polystyrene resins" Anal. Chem. 30, page 1185 (1958) with the use of liquid chromatography. It was found that leucine, glutamic acid, aspartic acid and glycine were the most frequently occurring amino acids in the peptide chain.
The most simple way for an immunological characterization of the substance is a known diffusion process wherein an antigen, i.e. the protein, and an antibody, directed against the protein, or the antiserum without enriched antibody diffuse towards each other in a carrier medium, for example agar. When the two reaction components meet each other in a favorable ratio, a visible precipitate is formed. With this knowledge, it is obvious to the exerpt that all immunologic techniques for the detection and determination of the novel tissue protein and of the antibodies directed against said tissue protein can be used.
It is another object of the invention to provide processes for the preparation of the aforesaid protein, which processes comprise fractionating organ extracts, generally aqueous extracts of organs containing the said protein, with consideration of the following criteria.
The protein can be precipitated with neutral salts. With ammonium sulfate, which is generally used for precipitations of this kind, the protein can be precipitated, with a saturation concentration of the salt of from 30 to 60%, in a pH range near the neutral point.
According to its molecular weight, the protein can be isolated by measures suitable for separating substances having a molecular weight in the range of from 35,000 to 50,000, preferably by gel filtration of ultrafiltration.
At a neutral or weakly alkaline pH, the tissue protein can be adsorbed on weakly basic ion exchangers. In this process a relatively weakly concentrated buffer solution is used because an increase of the salt concentration or a reduction of the pH value can prevent adsorption. On the other hand, this behavior makes it possible first to adsorb the tissue protein and then to elute it again with the use of higher concentrated salt solutions of buffer solutions with reduced pH.
It has been found that the tissue protein cannot be precipitated with water-soluble organic bases of the acridine and quinoline series as generally used in precipitation processes. With the concentrations generally used in these processes, the protein remains in the aqueous supernatant. Hence, it is possible to use an acridine base, for example 2-ethoxy-6,9-diamino-acridine lactate, or a quinoline base, for example bis(2-methyl-4-aminoquinolyl-6)-carbamide hydrochloride, for precipitating accompanying proteins, while the tissue protein of the invention remains in the supernatant.
Similar considerations are valid for the use of hydroxyl apatite as an adsorbent for proteins. The tissue protein of the invention does not show a specific affinity for hydroxyl apatite, whereas a series of accompanying proteins are held back by hydroxyl apatite.
Having knowledge of the electrophoretic mobility, preparative zone electrophoresis can be used for the concentration or isolation of the tissue protein.
The affinity of the tissue protein as a result of its immunological behavior can be utilized to concentrate the protein with the aid of so-called immuno-adsorption processes. To this end, an immuno adsorbent, i.e. an antibody for the tissue protein bound to a carrier, can be prepared which specifically adsorbs the tissue protein. The protein can then be eluted again by changing the medium conditions.
By a selected combination of the aforesaid methods, which are suitable, on the one hand, to concentrate the tissue protein and, on the other, to separate it from the other accompanying substances, it is possible to isolate the protein of the invention. One of the objects of the present invention is, therefore, a combination of individual concentrating steps and processes for the purification of the tissue protein.
The process for concentrating the tisue protein comprises at least one of the following treatments of organ extracts or solutions obtained therefrom and containing the tissue protein and the subsequent isolation of the fraction enriched with tissue protein:
(a) Addition of water-soluble derivatives of an acridine or quinoline base, preferably 2-ethoxy-6,9-diamino-acridine lactate, in a pH range of from 5 to 10, preferably about 8, to a final concentration of about 0.8% (weight to volume), whereby the tissue protein substantially remains in the supernatant.
(b) Addition to neutral salts until the tissue protein is precipitated, preferably ammonium sulfate at an approximately neutral pH, until 30 to 60% of the saturation concentration of ammonium sulfate has been reached.
(c) Adsorption of the tissue protein on a weakly basic ion exchanger, for example diethylaminoethyl cellulose at a conductivity of the solution of 0 to 2 mS and with a neutral or weakly alkaline pH, for example by using about 0.01 molar buffer of a pH of about 8, preferably tris-hydroxymethylaminoethane--HCl, with subsequent elution of the tissue protein by shifting the pH to a value below 7.0 or by increasing the conductivity to a value above 5 mS.
(d) Separation on the basis of the molecular size (molecular sieve fractionation), preferably by gel filtration in a column filled with a polymer having an appropriate pore size, for example dextran cross-linked with epichlorohydrin, prepared and sold under the name of SEPHADEXÛ by Messrs. Pharmacia, Uppsala, whereby proteins having a molecular weight of about 50,000 are concentrated; products such as ULTROGELÛ by LKB, Bromma or BIO-Gel PÛ by Bio-rad Laboratories, Richmond, Calif. being likewise suitable.
(e) Adsorption with hydroxyl apatite. Under the conditions generally maintained in preparative biochemistry, the tissue protein is not adsorbed by hydroxyl apatite and, therefore, this substance is a suitable reagent to remove accompanying tissue proteins from solution. The adsorption is suitably carried out at a pH near the neutral point of the solution and the conductivity of the solution is maintained at about 1 mS.
(f) Preparative zone electrophoresis. A solution containing the protein and suitable for an electrophoresis of protein, preferably an alkaline buffer solution such as a sodium diethyl barbiturate buffer of pH 8.6, ion strength 0.1, is introduced into an apparatus for preparative electrophoresis, for example as described by N. Heimburger and R. Schmidtberger in "Behringwerke-Mitteilungen," number 43, pages 83 et seq, more particularly pages 119 to 120, comprising a horizontal open trough in which electrophoresis is carried out on a carrier material. To dissipate the Joule's heat generated in the electrophoresis, the carrier material is cooled to a temperature below 10¯ C. As carrier material, substances which are inert towards proteins are used, preferably polyvinyl chloride or copolymers of vinyl chloride in the form of fine granules.
It proved advantageous to carry out the electrophoresis in the alkaline pH range, preferably at about pH 8.6, at an ion strength of from 0.08 to 0.12 and a field intensity of 4 to 6 volts per cm. Using 0.1 molar sodium diethyl barbiturate buffer of pH 8.6, the tissue protein migrates in the electrical field into the region of the plasma proteins classified as α2 and β1 zones.
To obtain the high molecular weight α-globulins, this zone is cut out and the desired substance is removed from the inert carrier material by elution with water or aqueous salt solutions, for example sodium chloride solution of 0.5 to 1% strength. In a last purification step, the eluate can be subjected to a gel filtrate, preferably after having been concentrated on an ultrafilter.
For the preparation of the novel tissue protein, several of the aforesaid measures can be combined and, after each step, the fraction containing the tissue is further treated while the remaining fractions are rejected.
Without constituting any limitation, the following description illustrates, by way of example, a possible mode of preparation of the tissue protein from parenchymatous tissue.
Human placentae are comminuted and extracted with water or a dilute salt solution of less than 10% strength, preferably a 0.5% neutral salt solution, for example of sodium chloride. About 1 to 5 liters of extraction solution are preferably used for 1 kg of placenta. The undissolved portions are separated from the extract by centrifugation or filtration.
It proved advantageous to isolate the tissue protein at a temperature below room temperature, for example 10¯ C. To the neutral or weakly alkaline placental extract, which is preferably adjusted to a pH of about 8, there is then added a water-soluble derivative of an acridine base, for example 2-ethoxy-6,9-diamino-acridine lactate up to a concentration of 0.8Ý0.4%, or a water-soluble derivative of a quinoline base, preferably bis(2-methyl-4-aminoquinolyl-6)-carbamide hydrochloride, up to a concentration of 0.3Ý0.15%. The precipitate formed is rejected. The supernatant contains the major proportion of the tissue protein to be concentrated. The precipitating agent in excess is expediently separated by adding compounds which form a precipitate therewith. The acridine bases, for example, form difficulty soluble compounds with halides so that the addition of sodium chloride offers itself for separating the acridine base. A satisfactory separation is achieved with a concentration of about 3 to 7% (wt/v) of sodium chloride. The supernatant protein solution is then admixed with a salt suitable for the precipitation of proteins in an amount such that the tissue protein is caused to precipitate. To this end, ammonium sulfate is preferably used in an amount to maintain a saturation concentration of said salt of 40 to 60%, preferably 40 to 50%. The precipitate formed after the addition of ammonium sulfate is separated by centrifugation or filtration and then redissolved in water. Generally, precipitates of this type obtained by salt precipitation include residues of the precipitating agent and, therefore, it is recommended to perform a dialysis thereafter. The protein solution is dialyzed suitably against a little concentrated buffer solution, for example as used in ion exchange chromatography, which makes possible an adsorption of the tissue protein on a weakly basic ion exchanger.
The dialyzed solution of the tissue protein is then mixed, in a weakly concentrated buffer medium, with a weakly basic ion exchanger, for example diethylaminoethyl cellulose and, after adsorption of the tissue protein on the ion exchanger, the latter is separated from the remaining solution, for example by filtration. If the adsorption is carried out in a weakly alkaline medium (about pH 8.0) with a buffer having a concentration of approximately 0.01 mol per liter, the ion exchanger can be washed with a 0.02 molar buffer of slightly reduced pH (about 6-7). The elution buffer may have a total salt concentration of about 0.2 mol per liter.
It is advantageous to add to the eluate an amount of neutral salt sufficient to precipitate the protein in the dilute solution. By dissolving the precipitate the protein is then obtained in concentrated form. For further purification the dissolved protein can then be subjected to a gel filtration. Fractions highly enriched with the desired tissue protein are obtained by using for the gel filtration a three-dimensionally cross-linked dextran of Messrs. Pharmacia known by the name of SEPHADEXÛ, Type G 150, preferably in column chromatography. For the elution buffer, solutions common in biochemistry can be used, to which neutral salts can be added to improve the fractionating effect, for example 0.01 to 0.1 mol of tris-hydroxymethylaminomethane hydrochloric acid buffer with an addition of 0 to 5 mols of NaCl, which solutions have an approximately neutral to basic pH. In this elution process the eluted fractions are analyzed, preferably by immunological methods, to find the tissue protein and the fractions containing the same are separated and collected.
The eluates resulting from gel filtration also contain the tissue protein in dilute form and, therefore, a concentration by precipitation with neutral salts as described above can be performed.
The solution of the tissue protein obtained by re-dissolution of the salt precipitate is dialyzed against a strongly diluted buffer solution and thereby adjusted to conditions which permit adsorbing on hydroxyl apatite as many as possible of the impurities still present. Weakly concentrated phosphate buffers, for example about 0.005 molar sodium phosphate buffer having a pH in the range of from about 6.5 to 7.0 are especially suitable. When the protein solution comes into contact with the hydroxyl apatite, the tissue protein of the invention substantially remains in solution. Hence, in the following process step the hydroxyl apatite, which is preferably in a column, need not be considered any more. The solution passed through the hydroxyl apatite column contains the desired tissue protein in further enriched and purified form.
For further concentration of the tissue protein, the ion exchange chromatography can be repeated. However, at variance with the steps described above, the ion exchanger is introduced into a column, the solution containing the tisssue protein is charged to the column, and the column is eluted with a salt solution of increasing gradient. The process steps listed above by way of example yield the tissue protein with a purity of over 99%.
It is also possible, of course, to obtain the tissue protein with the same degree of purity by combining different measures, for example by inserting additionally a preparative electrophoresis or an immuno adsorption.
The tissue protein prepared according to the invention has antigenic properties. An immunization of animals performed therewith according to known methods leads to the formation of specific antibodies in the blood of the immunized animals. Sera can be obtained therefrom by usual methods and enriched with the antibodies contained therein.
Owing to its tissue specificity, the substance of the invention can be used in diagnostics. Tissue diseases can be detected by the migration of the protein from the tissue into the blood stream. To this effect, essentially the known immunological modes of detection using the specific antibodies for the tissue protein may be used.
The following example illustrates the invention.
150 kg of deep-frozen placentas were comminuted and extracted with 150 l of a 0.5% aqueous sodium chloride solution. The extract was adjusted to pH 8 with 2 N sodium hydroxide solution and 50 l of a 3% aqueous solution of diaminoethoxyacridine lactate were added. After a waiting period of 1 hour, the supernatant, which contained the tissue protein, was siphoned off, 5% of solid sodium chloride (11 kg) were added to precipitate the dissolved diaminoethoxyacridine lactate, the mixture was filtered, 30%, calculated on the weight of the liquid, of solid ammonium sulfate were added and the liquid was thoroughly stirred. After 1 hour, the precipitate was filtered off.
500 g of the precipitate collected on the filter were dissolved in 500 ml of distilled water and dialyzed against a 0.01 molar tris (hydroxymethyl)-aminomethane hydrochloric acid buffer solution of pH 7.0 containing 0.05% of sodium azide. The dialyzed solution was centrifuged and the supernatant was made up to 2,000 ml with the same buffer solution, adjusted to pH 8.0 with 0.1 N sodium hydroxide solution and stirred for 1 hour with 500 g moist diethylaminoethyl cellulose (Messrs. Serva, Heidelberg).
The diethylaminoethyl cellulose was separated from the solution by filtration, washed twice, each time with 1 liter of 0.01 molar tris(hydroxymethyl)-aminomethane-hydrochloric acid buffer of pH 8.0, and then eluted three times, each time with 500 ml of 0.02 molar tris(hydroxymethyl)-aminomethane-hydrochloric acid buffer of pH 6.5 containing 0.85% of sodium chloride and 0.05% of sodium azide.
Next, 30% of ammonium sulfate, calculated on the liquid weight, were added to the combined eluates and the whole was stirred. The precipitate containing the tissue protein was separated and dissolved in 300 ml of distilled water and subjected to gel filtration with SEPHADEXÛ G-150 using 0.1 molar tris-(hydroxymethyl)-aminomethane-hydrochloric acid buffer of pH 8.0 containing 1.0 mol of sodium chloride per liter (100:20 cm column). In the gel filtration the tris buffer was used as a suspension medium for the carrier for the gel filtration, i.e. SEPHADEXÛ G-150 and as eluting agent for the tissue protein dissolved in distilled water and charged to the column filled with SEPHADEXÛ.
The eluates were analyzed with specific antiserum for the tissue protein, the fractions containing the tissue protein were collected and the proteins precipitated again as described above with 30% solid ammonium sulfate.
For further purification, hydroxyl apatite (column 3æ23 cm) and a 0.005 molar phosphate buffer of pH 6.8 were used and then chromatography on diethylaminoethyl-SEPHADEXÛ (Pharmacia) (column 3æ23 cm) using a tris-HCl buffer of pH 7.0 was performed. For the elution a salt gradient of 0 to 2% NaCl was used. 40 mg of the novel tissue protein were obtained having a purity of 99.5%.
______________________________________ Variation Mol % coefficient (%)______________________________________lysine 5,64 12.7histidine 1.05 15.0arginine 3.83 9.2aspartic acid 9.84 6.8threonine 4.33 15.7serine 4.78 8.2glutamic acid 11.29 3.5proline 6.41 9.2glycine 8.65 9.8alanine 7.51 6.4cystine/2 2.11 12.6valine 6.68 7.9methionine 0.98 14.5isoleucine 3.32 7.4leucine 14.43 4.2tyrosine 5.10 14.9phenylalanine 3.08 13.5and tryptophan 1.14 11.4______________________________________
|Cited Patent
|Filing date
|Publication date
|Jun 15, 1965
|Nov 5, 1968
|American Cyanamid Co
|Concentration and purification of growth factor-placental origin (human)
|Apr 2, 1968
|Feb 24, 1970
|American Cyanamid Co
|Preparation of placental albumin
|Jan 17, 1974
|Sep 9, 1975
|Behringwerke Ag
|Process for isolating a fibrin stabilizing factor from human placenta
|Mar 14, 1974
|Oct 7, 1975
|Us Health
|Isolation of glucocerebrosidase from human placental tissue
|Dec 21, 1973
|Jan 6, 1976
|Behringwerke Aktiengesellschaft
|Process for isolating a fibrin-stabilizing factor
|Oct 24, 1974
|Apr 19, 1977
|Behringwerke Aktiengesellschaft
|Steroid-binding globulin and process for preparing it and antibodies thereto
|Jul 1, 1975
|Aug 9, 1977
|Behringwerke Aktiengesellschaft
|AP-glycoproteins and process for isolating them
|Dec 9, 1977
|Mar 4, 1980
|Behringwerke Aktiengesellschaft
|Pregnancy-specific β1 -glycoprotein and process for isolating it
|Bohn et al., Chemical Absts., vol. 76:70,559g (1972).
|Bohn et al., Chemical Absts., vol. 77:118,184f (1972).
|Bohn, Chemical Absts., vol. 76:32,096w (1972).
|Bohn, Chemical Absts., vol. 77:162,901m (1972).
|Bohn, Chemical Absts., vol. 83:92,637c (1975).
|Centonze et al., Chemical Absts., vol. 51:16,804e (1957).
|Centonze et al., Chemical Absts., vol. 55:23,724f (1961).
|Mansfield et al., Chemical Absts., vol. 65:9157a (1963).
|Citing Patent
|Filing date
|Publication date
|Sep 27, 1979
|Nov 17, 1981
|Behringwerke Aktiengesellschaft
|Ubiquitary tissue protein PP8
|Dec 17, 1979
|Nov 24, 1981
|Behringwerke Aktiengesellschaft
|Placenta-specific tissue protein PP10
|Dec 29, 1980
|Sep 7, 1982
|Behringwerke Aktiengesellschaft
|New protein PP15, a process for its preparation and its use
|Apr 8, 1981
|Jan 11, 1983
|Behringwerke Aktiengesellschaft
|Protein PP9, process for its enrichment and its isolation and its use
|Mar 11, 1982
|Sep 6, 1983
|Behringwerke Aktiengesellschaft
|Protein and process for isolating it
|Jul 29, 1983
|Aug 28, 1984
|Behringwerke Aktiengesellschaft
|Protein (PP17), a process for concentrating and isolating it and its use
|Aug 18, 1983
|Feb 19, 1985
|Behringwerke Aktiengesellschaft
|New protein PP13 extracted from human placental tissues and use thereof
|Apr 24, 1984
|Mar 26, 1985
|Behringwerke Aktiengesellschaft
|Tissue protein PP4, a process for isolating it and its use
|Sep 21, 1984
|Jun 18, 1985
|Behringwerke Aktiengesellschaft
|Membrane-associated proteins (MP2), a process for obtaining them, and their use
|Feb 7, 1985
|Jul 8, 1986
|Behringwerke Aktiengesellschaft
|Tissue protein PP19, a process for obtaining it, and its use
|Sep 19, 1986
|Mar 22, 1988
|Kowa Co., Ltd.
|Placenta-derived anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
|May 17, 1985
|May 24, 1988
|Behringwerke Aktiengesellschaft
|Isolated tissue protein PP18
|Jan 15, 1987
|May 31, 1988
|Kowa Co., Ltd.
|Thrombin-binding substance and process for preparing the same
|Jun 9, 1995
|Dec 21, 1995
|Aktsionernoe Obschestvo Zakryt
|Medicament base
|U.S. Classification
|530/389.1, 530/419, 530/417, 436/543, 530/851, 530/392, 530/394, 424/533, 424/583, 530/386, 530/806, 530/420
|International Classification
|Cooperative Classification
|European Classification
|Oct 3, 1997
Owner name: BEHRING DIAGNOSTICS GMBH,GERMANY
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BEHRINGWERKE AKTIENGESELLSCHAFT;REEL/FRAME:009168/0310
Effective date: 19970721


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text:
A woman was in bed with her lover when she
heard her husband opening the front door.
"Hurry!" she said, "Stand in the corner."
She rubbed baby oil all over him,
Then dusted him with talcum powder.
'Don't move until I tell you,'
She said. 'Pretend you're a statue.'
'What's this?' the husband inquired
As he entered the room..
'Oh it's a statue,' she replied.
'The Smiths bought one and I liked it
So I got one for us, too.'
No more was said,
Not even when they went to bed.
Around 2 AM the husband got up,
Went to the kitchen and returned
With a sandwich and a beer.
'Here", he said to the statue, "have this".
I stood like that for two days at the Smiths
And nobody offered me a damned thing!
How much of this joke is taken from your own life??
Another good one Donny boy. Anyone want a sandwich?
No thanks, but I will take the beer.


text:
Find out more about the Parish Giving Scheme.
Click here to find out more about elections to General Synod
Work is well under way to produce Parish Mission Action Plans.
Whether you are someone looking for a church to get married in, wanting to grow closer to Christ, or a church leader looking for resources and support, we hope this website provides you with the information and resources you need.
© Copyright 2015 Winchester Diocesan Board of Finance. All rights reserved. Site designed by Cubik


text:
Cumbria battered by severe gales
Last updated at 08:07, Thursday, 13 February 2014
Strong winds battered Cumbria as the latest winter storm moved across the country.
There was an unconfirmed report that part of the roof at Southfield school in Workington had been blown off and an area of the school closed as a result.
The school yesterday (Wednesday) afternoon confirmed there was an "issue" with the roof but declined to give any details. The county council said damage was "very minor" and repairs should be completed today.
A police spokeswoman said they received a call from the school just after 1.30pm saying that a large area of the metal roof was lifting due to the wind and that "precautions" had been put in place for students.
A flood alert was issued by the Environment Agency for the Rivers Caldew and Petteril. It warned that flooding was possible for areas including Greystoke, Newton Reigny, Plumpton, Stockdalewath, Sebergham and Denton Holme.
Alerts were also in place on the upper and lower River Eden, the upper River Derwent, Stonethwaite Beck and Derwent Water and the coast from Gretna all the way down to Millom.
At one point, about 5,000 customers were without electricity, mainly in south Cumbria, said Electricity North West.
That has since been reduced to 1,200 and the firm says supplies to those properties should be reinstated today.
The company has drafted in extra engineers to deal with the problems.
The firm said last night: "As the weather is still severe it is too dangerous for our engineers to repair the overhead power lines but we will be switching the network and getting people back on as soon as possible."
A spokeswoman added that the company's partners, the British Red Cross, were on standby to "offer extra support to vulnerable customers".
Residents across the county were advised to be prepared.
A recent image from @eumetsat showing the latest #ukstorm battering our shores. #c4news pic.twitter.com/gCYYtD3tPB
— Liam Dutton (@liamdutton) February 12, 2014
It had been predicted that parts of northern and western England would bear the brunt of the problems, with gusts of 60 to 70mph widely, potentially reaching 80 to 90mph in exposed areas. In fact, a speed of 112mph was recorded at Great Dun Fell.
The A66 was last night (Wednesday) closed in both directions - not just to high-sided vehicles - between the A685 junction in Brough and the A67 junction in Bowes due to high winds.
The B5300 Allonby to Silloth road was also closed at Dubmill Point.
The M6, between junctions 36 and 40, was closed to high-sided vehicles due to the high winds and the M6 northbound was closed between junctions 38 and 39 due to an accident.
This morning, the M6 north between junction 44 Carlisle North and junction 45 Gretna is closed, due to an overturned HGV.
A fallen tree has blocked the A6071 Longtown to Brampton road at the junction near Walton.
Police advised motorists to travel only if necessary. Drivers should slow down and drive in accordance with the conditions, not the speed limit, they said.
Inspections of the Cumbria coastal rail line are taking place at Parton, near Whitehaven, today after sea defences were breached.
Speed restrictions were in place as a precaution on Carlisle-Preston/Crewe, Carnforth/Barrow-Carlisle (Cumbrian coast line), Settle-Carlisle services from 4pm until midnight yesterday, Northern Rail said.
The restrictions meant extended journey times and for connecting services run by other operators.
A spokesman said yesterday: "So, please only travel if necessary. If you do have to travel today make your journey earlier if you can or allow more time if you are travelling this evening. Early indications are that road networks may also be affected, particularly in Lancashire and Cumbria.
"Tickets dated for travel today on north west routes will be valid for travel tomorrow."
Yesterday afternoon, Virgin Trains advised customers not to attempt to travel. Customers already on its services were taken to the nearest station.
All Virgin services out of London Euston were suspended due to various problems across the rail network. Virgin had said earlier that services would stop for two hours on the West Coast Mainline to Carlisle from 7pm yesterday.
Pupils were sent home from Millom School and out of hours activities or meetings today cancelled as a precaution yesterday.
Windermere Lake Cruises cancelled services due to the adverse weather. The company was hoping sailings would return to normal today.
Drivers were yesterday (Wednesday) morning urged to take care on Cumbria's icy roads. The warning, from police, comes after a night of roads chaos caused by snow and ice. One lane of the M6 southbound at Plumpton was blocked this morning after a vehicle skidded on ice. There are not believed to be any injuries.
Snow showers were expected in the north of England, including Cumbria. Accumulations of between two and five centimetres of snow were likely on ground above about 150 to 200m, with 10cm or more above 300m.
The Met Office warned that a "swathe of exceptionally strong winds" was expected to affect some areas of the north west last night, particularly in coastal areas.
Winds were expected to gust to 80mph widely, possibly causing structural damage, bringing down trees and also leading to loss of power supplies. In addition, large waves were likely to affect some coasts.
Members of the public were warned to take action to alter their travel arrangements during storm force winds.
The Met Office has also issued a yellow warning for snow in Cumbria.
Forecasters said rain would turn to snow over the high ground last night, becoming showery overnight into today (Thursday), with a risk of some snow to lower levels later.
Up to 3cm of snow are possible above 150-200m and 5-10cm above 300m. Combined with severe gales, blizzards are likely to affect higher level roads, they add.
First published at 14:26, Wednesday, 12 February 2014
Published by http://www.newsandstar.co.uk
Have your say
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- Cumbrian council set to axe chief executive job (14 comments)
- Former Carlisle hotel one of England's most at risk buildings (21 comments)
- Thirty new Cumbrian entries in Camra's latest 'beer bible' (5 comments)
- Parents furious as children kept out of lessons for flouting uniform rules (85 comments)
- 'Rude' Cumbrian town name banned at national WI fair (44 comments)
- Carlisle's Toni & Guy hair salon shuts (40 comments)
- Plans unveiled to revamp Carlisle's Castle Way (139 comments)
Court & crime
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- Driver lost control and crashed through Carlisle charity shop window
- Carlisle man jailed for lying for cocaine trafficker dad
- Cumbrian man convicted of raid on Dartmouth gift shop
- Men who turned Cumbrian mansion into cannabis farm jailed for total of almost 40 years
Anne Pickles
Quick links
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- Massive cost of dealing with stray dogs in Cumbria revealed (2 comments)
- Ex-Lookaround newsreader begins life as woman she always wanted to be (16 comments)


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text:
Mok'House B&B
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- Shuttle bus service
- Continental Breakfast
- Suites
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Day 52 (Perugia/Rome)
We woke up early again this morning to say goodbye to akashi and Georgie. After that we did the little packing we had to do, went out for breakfast near our hotel and then checked out and hit the road. It was meant to be a long drive but even though mum and dad got lost it only took two hours. Me, Nim and atifa all sat in the back listening to our iPods and acting like siblings. We stopped at an auto grill on the way but apart from that it was ...
Day 22: Vaticano!
... all over the place. Other rooms were filled with tapestries and maps of the ancient Catholic Churches and territories. Throughout the museum there was fresco after fresco all over the walls and ceilings. After an hour of walking through the crowds we finally reached the Sistine Chapel. Entering this my eyes were bombarded by frescos of angels and popes and more beautiful objects. It was dark and no photography ...
Gladiator City
... get into our room early and have a shower and put some clean cloths on. Enough of that boring stuff..... Rome what can we say. I'm sure everyone has their own picture of Rome in their mind in what they might find or how they would like to find it. For all the gladiator movie watchers sorry it's not like that. Rome is a city that mixes old with new and ancient and contemporary. With each corner you turn there is ...
The Vatican & Sistene Chapel
... I have now posted a parcel home from every country so far - England, Ireland and Italy. The language difficulties presented problems as well as the fact that they do things differently but they were so SLOW!!!!! Dad finally returned about 7pm and had done lots of walking, as well as bus travel. His walking also involved lots of stairs up and down the mountain. We were off to dinner and then had to get packed for the start of our Espana ...
Pope On A Rope
... 000 people. Staples Center can hold almost 20,000. Michaelangelo's Pieta is one of the most moving works of art I've ever seen. Lindsay and I were both moved by Roxanna's description of the piece, showing not only Jesus and Mary's relationship, but also a mother and son relationship. I looked at it and saw a powerful portrait of a mother holding her son's tortured body, a mother mourning the loss of her child. With Mother's Day in mind, I ...


text:
30 Apr
Welcome to our new website
I am delighted to welcome you to our new school website.We are a three form entry junior school for ages 7 -11, with extended provision of a Breakfast Club from 7:30am. In addition, there are many after school clubs run by staff and volunteers.
Our school motto "Respect, Inspire, Learn Together" reflects our high aspirations and ambitions for children and staff and there is a determination to ensure that all pupils and adults in the school reach their full potential. We are continually looking for ways in which we can improve the school further.
Hempstead Junior is a happy and inclusive school of which our children are proud. We want them to have special and happy memories of their time at Hempstead Junior School.
Sharon Smith, Headteacher
See the Headteacher's full welcome here


text:
India vs Australia: Pro-Ealam outfit protests Sri Lankan umpire officiating match
Top Stories
About 50 activists of a pro-Ealam outfit were arrested today for staging protest outside M A Chidambaram stadium here against a Sri Lankan umpire officiating in the first India-Australia cricket Test match.
Members of the little-known 'Thanthai Periar Dravidar Kazhagam' were arrested for staging protest against the presence of Lankan umpire Kumara Dharmasena, police said.
The black outfit wearing activists raised slogans against Sri Lankan president Mahinda Rajapakse for the alleged cold-blooded killing of slain LTTE chief V Prabhakaran's 12-year-old son Balachandran and objected to the presence of a Lankan official for the sporting event.
Meanwhile, another pro-Ealam outfit, the "May 17 Movement", staged a protest outside TNCC headquarters Sathyamurthy Bhavan for the alleged killing of Balachandran.
Around 25 activists were detained, police said.


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Drivers get stoned and take road test to evaluate Washington's marijuana DUI laws in entertaining video
Three people smoked so much marijuana that one person couldn't touch their nose and another laughed and said 'I'm stoned' - and then they got behind the wheel of a car.
It was part of an experiment for a local television news program to test Washington's new DUI laws for marijuana, which just became legal there and in Colorado.
The two men and one woman, who soon lost the ability to drive safely after smoking just a few hits of weed, were on a closed course and had a driving instructor who had his own brake pedal in case they lost control.
Scroll down for video
For research: Three drivers smoked marijuana and then drove to test Washington state's DUI laws following the legalization of the drug
Okay at first: Addie Norton, 27, a regular marijuana smoker who was above the legal limit initially drove safely
Oops: Addy at one point backed up into a cone, which police said suggests she would have struck a person, another vehicle or other object
One of the drivers, Addie Norton, 27, is a medical marijuana patient and frequent user who arrived at the test three times above the legal limit because she smoked at home, said KIROTV.com.
The limit is five nanograms of THC per milliliter of blood.
While everyone is aware of the standard for how much is too much alcohol to consume before driving, there's much more debate over how to determine whether someone is too stoned to drive.
For example, during Norton's first road test, while she was above the limit, she still drove fine.
The other two volunteers, Dylan Evans, 34, and Jeff Underberg, 56, both had no marijuana in their systems but said they occasionally used the drug.
All three smoked .3 grams of a marijuana strain called 'Blueberry Train Wreck' before getting behind the wheel.
Police were on site to monitor as well as offer their opinions on whether they'd pull over the drivers.
Happy to help: Another one of the drivers, Jeff Underberg, said he occasionally smokes marijuana
Third man up: Dylan Evans, 34. smokes on the weekends and was able to drive normally after just a little pot
After smoking the first round, both Evans and Underberg said they felt stoned.
'I'm really buzzed, yeah,' said Underberg while Evans added he was feeling 'Relaxed and buzzed.'
Norton, a frequent smoker, said, 'I can't feel it yet.'
But when she got on the course, she was driving slower than normal and struck a traffic cone.
'I hit a cone. I see it in the rearviewmirror, ah,' she realized.
A test revealed she was now seven times above the legal limit at 36.7 nanograms.
Not good: This police officer on scene is not happy with the way Norton was driving after striking a parking cone
The driving instructor, Mike Jackson, claimed she was driving okay.
'I wouldn't pull her off the road yet. No, not at this point,' he said.
Then Evans started driving. After smoking the small amount of pot, he was more than five times the legal limit with a reading of 26 nanograms but drove normally.
Underberg also measured above the limit with a 21.7 nanograms. But his driving, while a little slower than normal, was excellent, Jackson said.
'He did real well,' said Jackson.
Too much: After smoke two more times, Norton was now 11 times above the legal limit and dangerous behind the wheel
The three drivers smoked two more times, for a total of .9 grams of pot, and drove. Each was now unable to safely operate a car.
Norton was now 11 times the legal limit and thrilled for the chance to drive.
'One time in your life you get to do this, you should do it. You should do it (laughs). Yeah. That's awesome,' she said.
'I'm starting to get it, oh yeah. I can feel it in my eyes. I can feel it in my body and my head,' she said.
She did not make any major mistakes and the police officer on site, Dave Claridge, wasn't sure he would have pulled her over if he spotted her on a road.
'(She's) borderline,' said Claridge.
Touch your nose: Officer Claridge took all three for a full field sobriety test
The two men had more trouble driving at this point.
Evans was confused and made a turn that took him off the designated course.
'What, is this cone in the middle?' he asked.
While trying to make a turn, Jackson was forced to grab the wheel to keep the car from striking a cameraman on scene.
'If I was to be watching him on a road, I would have stopped him,' Claridge said.
VIDEO Stoned drivers swerve their way through the course
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text:
The Mittenza
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Swiss Education System in a DAY!
... the teacher was able to balance her time with each grade. Many of the students were working on the metric system. The last lesson we watched was an eighth grade English lesson. This lesson was fun because we could understand what was going on much better! Fun to hear. Dinner at the Kellner's was the traditional Swiss dish of raclette which included boiled potatoes and raclette cheese. It was a beautiful evening sitting the backyard eating dinner with the ...
The Amazing Race
... from the previous class. It was only a ten minute test but it was very interesting because the students first looked at the quiz and tried on their own and then were quietly discussing the problems with their peers. After each student finished they were handed the guide to see how to get the correct answer. This then transitioned to the next lesson of the day. It was very similar to our classes and how we teach, including the discussion. The ...
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I need to mark Basel on the map & would also like to say to anyone reading this that might be planning a trip to the area, we stayed at The Passage hotel - it was EXCELLENT ! and I would highly recommend it; awesome shower, big rooms, comfy bed, mini fridge (if you can afford that sort of thing), a fantastic gym downstairs, and just a hop, skip, and jump away from anything and everything you could possibly want. ...
Happy Birthday Jake
... plus side it meant we knew where and what to find in the town. We crossed the medieval bridge that crosses the river Rhine one half being in Germany the other half in Switzerland. We went to Woolworths which although signed like we remember it was just a glorified pound shop. Although wasn't that all it was anyway? At the supermarket we stocked up on supplies and managed ...
... At 8:30 pm it was time to say 'Adios' and get on the train back to Brig. This time we did not miss the train. We made it back to Naters by 11:00 pm. Now we rest up for the next adventure. We will spend the weekend at a friend's house in the ...


text:
A 61 year-old Burkburnett man is facing multiple charges
A 61 year-old Burkburnett man is facing multiple charges
Two separate accidents on I-44 send three to United Regional Hospital
Summer is right around the corner and with the temperatures in North Texas easily in the triple digits we all need a place to cool off. Burkburnett residents wanted a new pool and they sure got what they asked for.
An unidentified man led Burk police on a brief high speed chase shortly before 8 am this morning.
Burk police arrest woman in connection with shooting
Burk man in custody after Saturday afternoon chase
If you are a regular listener of Kidd Kraddick in the Morning or you've tuned in once or twice in the last couple months chances are you've heard of their Classroom Musical contest...