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News about Terrorism, including commentary and archival articles published in The New York Times.
Chronology of Coverage
Oct. 12, 2015
Thousands gather in Ankara, Turkey, to mourn victims of worst terrorist attack in country's modern history; many of those who gather are Kurds, as they were primarily affected by two explosions detonated at peace rally, and many blame government of Pres Recep Tayyip Erdogan for carnage. MORE
Oct. 8, 2015
Three suicide bomb attacks kill 18 people in northeastern Nigeria, while Nigerian troops kill 100 members of Islamic extremist group Boko Haram after insurgents attack military camp. MORE
Oct. 7, 2015
Counterterrorism officials in Sydney, Australia, arrest four people in connection with shooting of police accountant Curtis Cheng by 15-year-old assailant Farhad Jabar Khalil Mohammad; motive has yet to be determined but incident is being considered terrorist attack. MORE
Oct. 1, 2015
Indian court sentences five Pakistani-trained terrorists to death and seven to life in prison, after seven-plus year trial, for carrying out 2006 Mumbai train bombings. MORE
Sep. 30, 2015
Pres Obama implores world leaders at United Nations to combat terrorists like Islamic State with ideas, jobs and competent governance, not just militarily; call is viewed by many as futile. MORE
Sep. 30, 2015
New York City Mayor Bill de Blasio's administration announces city will join Justice Dept's Strong Cities Network program for identifying and stopping terrorist recruitment efforts across country; American Muslim advocates have raised concerns about program, as have civil rights lawyers, as they say it focuses inordinately on law-abiding American Muslims and their activities. MORE
Sep. 29, 2015
Op-Ed article by Anne Speckhard underscores necessity of United States speeding up its refugee acceptance process, both for humanitarian reasons and for nation's security; notes longer refugees are in limbo, more likely they are to be radicalized by terrorist; points out that by accepting more Syrians in need of refuge, US could diminish pool of refugees from which Islamic State can recruit. MORE
Sep. 25, 2015
Mosque in Sana, Yemen, is struck by two bombs during prayers marking start of Muslim holiday Eid al-Adha; Islamic State claims responsibility for attack that kills at least 25. MORE
Sep. 24, 2015
Canadian court gives Chiheb Esseghaier and Raed Jaser life sentences for terrorist plot to derail passenger train; judge rejects Jaser's entrapment defense and rules Esseghaier, who is probably schizophrenic, was mentally sound during plotting of crime, which was recorded by FBI agent. MORE
Sep. 24, 2015
Nigerian military bans all moving vehicles, including animals used for transportation, in northeastern state of Borno during Muslim holiday, in effort to prevent Islamic extremists attacks like recent explosions in Maiduguri and Monguno that killed over 100 people. MORE
Sep. 22, 2015
Bomb-laden car explodes at gate of Somali presidential palace in Mogadishu, killing four and wounding another dozen; militant Islamist group Shabab claims responsibility. MORE
Sep. 22, 2015
Series of bombings in northern Nigerian town of Maiduguri and attributed to Boko Haram, kill over 100 people; attackers take advantage of Muslim holiday celebrations to maximize casualties, and cast doubt on government's continued assurances that Islamist extremist group is being stamped out. MORE
Sep. 18, 2015
Iran denies report it swapped five key Al Qaeda members, including operational leader Saif al-Adl, for kidnapped Iranian diplomat Nour Ahmad Nikbakht, held by network's Yemen affiliate; American official confirms trade, which experts fear will improve Al Qaeda's morale and resources, depleted by rise of Islamic State. MORE
Sep. 18, 2015
Oregon National Guard Specialist Alek Skarlatos is celebrated at Pentagon and White House for foiling terrorist attack in France in August; meets with Pres Obama, accompanied by two childhood friends, Airman First Class Spencer Stone and Anthony Sadler, who were also honored for their heroism. MORE
Sep. 3, 2015
Airman First Class Spencer Stone describes in interview how he and his friends Alek Skarlatos and Anthony Sadler foiled terrorist attack by gunman Ayoub El Khazzani on France-bound train during last leg of summer vacation in Europe; Stone is returning to United States after winning international acclaim for heroic act. MORE
Sep. 2, 2015
Media advocacy groups score terrorism charges brought by court in Turkey against two British Vice News journalists and an Iraqi news assistant; three are accused of knowingly helping an armed terrorist organization, which remains unnamed. MORE
Aug. 31, 2015
National security adviser Susan E Rice, during visit to Islamabad, urges Pakistan's leaders to do more to stop militants from using Pakistan's territory to launch attacks in neighboring countries. MORE
Aug. 28, 2015
Spotlight falls on Taqwa, local mosque in Algeciras, Spain, which authorities say was crucial part of Ayoub El Khazzami's turn to radicalism before he was foiled in Paris-bound train attack. MORE
Aug. 27, 2015
Saudi Arabian government arrests Ahmed al-Mughassil, who is accused of organizing 1996 bombing of building in Khobar Towers building complex that killed 19 United States airmen and left 400 people wounded; Mughassil was leader of Iranian-backed Saudi militant group Hezbollah al-Hijaz, and arrest underscores tensions between US and Iran even as two seek to secure deal regarding Iran's nuclear weapons. MORE
Aug. 27, 2015
Egyptian officials say gunmen killed two policemen on Sinai Peninsula; Sinai Province of the Islamic State claims responsibility, distributing images allegedly depicting shooting through social media. MORE
Aug. 26, 2015
Chief Paris prosecutor Francois Molins opens formal investigation against Ayoub El Khazzani for attempted murder in connection with terrorist act; says suspect watched Jihadist videos just before foiled attack on Amsterdam-Paris train. MORE
Aug. 25, 2015
Manhattan Federal Judge George B Daniels orders Palestinian Authority to post bond of $10 million and additional $1 million per month for duration of appeal of $655.5 million damages award in civil trial brought by families of American victims of terrorist attacks in Israel; ruling follows unusual 'statement of interest' by Justice Dept expressing concern for economic and political harm that high bond could cause to Palestinian Authority. MORE
Aug. 25, 2015
Editorial expresses alarm at ease with which Ayoub El Khazzani brought arsenal onto train traveling from Brussels to Paris, noting that European rail system seems particularly vulnerable to terrorist attacks by Islamist militant groups; underscores need for improved security measures on rail system in Belgium and France and throughout Continent. MORE
Aug. 24, 2015
European officials, still investigating thwarted terror attack on Paris-bound train, search for ways to keep region safe, without impacting everyday lifestyles of citizens; incident points up difficulty of protecting soft targets from terrorism; accused gunman Ayoub El Khazzani was identified as potential threat by at least two countries prior to incident. MORE
Aug. 24, 2015
Spencer Stone, Alek Skarlatos and Anthony Sadler, three American men who thwarted attack on Paris-bound express train, appear at news conference; offer account of how they wrestled down Moroccan suspect Ayoub El Khazzani, who was carrying AK-47, but deny suggestions that they are heroes. MORE
Aug. 23, 2015
American tourists Spencer Stone, Alek Skarlatos and Anthony Sadler attack and subdue heavily armed man on high-speed European train in France; although some are wounded in confrontation, intervention saves passenger lives; Moroccan suspect, whose name is not released is known to Spanish authorities as member of radical Islam movement. MORE
Aug. 23, 2015
French authorities identify Ayoub El Kahzzani as suspect in aborted attack on passenger train; Kahzzani is known to French and Spanish security agencies, and may have traveled to Syria in 2014; officials also say he may be connected with Verviers Islamic terrorist network, recently dismantled by Belgian authorities. MORE
Aug. 23, 2015
Attempted terrorist attack on a high-speed train in France raises questions about vulnerability of America's railroads and adequacy of current security measures. MORE
Aug. 23, 2015
Americans Anthony Sadler, Alek Skarlatos and Spencer Stone, who subdued an armed terrorist on a high-speed train traveling through France, discuss their boyhood friendships and the fateful decision to travel together in Europe that would lead them to become heroes. MORE
Aug. 22, 2015
American service members Spencer Stone and Alek Skarlatos tackle and detain suspected terrorist who entered high-speed train traveling from Amsterdam to Paris carrying AK-47 and handgun; Stone is severely cut by suspect, identified only as a 26-year-old man of Moroccan origin, and is receiving treatment at hospital; France's top antiterrorism investigators are trying to sort out sequence of events. MORE
Aug. 18, 2015
Egyptian Pres Abdel Fattah el-Sisi enacts counterterrorism law that allows security officers more immunity from prosecution, penalizes journalists if they contradict any official statements about militant attacks and expands surveillance powers of state. MORE
Aug. 15, 2015
Jaelyn Young and Muhammad Dakhlalla of Starkville, Miss, are arrested on suspicion of trying to travel from Mississippi to Syria to join Islamic State; friends and family are shocked to hear official charges, which could mean sentence of up to 20 years in prison. MORE
Aug. 15, 2015
Arab Bank agrees to pay damages to around 500 American plaintiffs, including victims of terrorist attacks in Israel, who filed suit alleging that bank supported terrorism by carrying out transactions for members of militant Islamic group Hamas; amount of settlement, carried out under federal Anti-Terrorism Act, has yet to be determined; banking executives say case sets worrisome precedent. MORE
Aug. 15, 2015
Abdurasul Hasanovich Juraboev, Brooklyn man accused of trying to fly to Istanbul in February with intent to fight for Islamic State, pleads guilty to conspiring to provide material support to terrorist organization; Juraboev faces maximum sentence of 15 years in prison. MORE
Aug. 13, 2015
Judge John Gleeson of Federal District Court in Brooklyn sentences Lawal Babafemi to 22 years in prison for providing material support to Al Qaeda in the Arabian Peninsula; says Babafemi's recent denunciations of terrorism helped him in receiving slightly less time than guidelines called for. MORE
Aug. 12, 2015
Editorial warns differing opinions within Obama administration over which country or entity poses greatest threat to America present obstacle to determining best way to protect United States; notes competing assessments stating that Russia, Middle East, North Africa, Al Qaeda or ISIS are most worrisome; holds administration faces formidable challenge in determining what its defense priorities are. MORE
Aug. 11, 2015
Obama administration requests judge lower bond for Palestinian Authority to appeal damages owed to families of Americans killed or harmed in six terrorist attacks in Israel between 2002 and 2004; cites worries that large payment would compromise Palestinian Authority's ability to operate, and thereby harm the region. MORE
Aug. 11, 2015
Federal prosecutors charge Nader Saadeh of Rutherford, NJ, with conspiracy and trying to provide material support for Islamic State group; Saadeh was detained in May in Jordan after authorities were told by Saadeh's acquaintance that he was trying to join terrorist group. MORE
Aug. 10, 2015
Security crackdown in Tunisia amid terrorist attacks and threats is raising concerns among citizens who fear that hard-fought liberties achieved during Arab Spring will vanish as government takes steps to combat jihadists. MORE
Aug. 5, 2015
Top federal officials are split over whether Islamic State or Al Qaeda poses gravest risk to United States homeland; Obama administration appears to be most concerned with Islamic State; debate will affect how government spends billions of dollars against terrorism, and how it assigns thousands of agents, analysis and troops to combat threat. MORE
Aug. 3, 2015
Attack by Israeli extremists that killed toddler and severely burned family members in West Bank village of Duma has turned glare on perception that Israeli authorities are far more lenient toward Jewish perpetrators of crimes than they are toward Palestinians; attack has raised question of how to maintain democratic values in society while at same time rooting out perpetrators of extremist violence within the culture. MORE
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Deadly Ankara Attack Not Enough to Unify a Polarized Turkey
Even in the face of a bombing that killed nearly 100 people, political leaders bickered among themselves rather than offer consolation.
October 13, 2015, Tuesday
MORE ON TERRORISM AND: Terrorism , Kurds , Sancar, Aziz (1946- ) , Ankara (Turkey) , Justice and Development Party (Turkey) , Kurdistan Workers' Party
Russia Says It Has Detained a Terrorist Cell in Moscow
Russia's main security agency said an "explosive device" was found in an apartment frequented by people who had trained with the Islamic State.
October 13, 2015, Tuesday
MORE ON TERRORISM AND: Terrorism , Federal Security Service , Putin, Vladimir V , Moscow (Russia) , Islamic State in Iraq and Syria (ISIS) , Bombs and Explosives
Ankara Bombings Prompt Rally Against Turkish Government
A day after the worst terrorist attack in Turkey's modern history, thousands of mourners gathered in central Ankara to lay carnations and rail against the government.
October 12, 2015, Monday
MORE ON TERRORISM AND: Turkey , Terrorism , Kurds , Demonstrations, Protests and Riots , Bombs and Explosives , Erdogan, Recep Tayyip , Ankara (Turkey) , Kurdistan Workers' Party
Explosions During Peace Rally in Ankara, Turkey's Capital, Kill Scores
Turkish authorities were investigating claims that the attacks, which also wounded 246, were the work of suicide bombers.
October 11, 2015, Sunday
MORE ON TERRORISM AND: Ankara (Turkey) , Terrorism , Demonstrations, Protests and Riots , Kurds , Kurdistan Workers' Party , People's Democratic Party (Turkey) , Justice and Development Party (Turkey) , Erdogan, Recep Tayyip , Turkey
Suicide Bombers Kill at Least 38 at Market and Refugee Camp in Chad
Chadian officials suspect that the attacks, which also wounded more than 50 people, were carried out by members of the Boko Haram extremist group from neighboring Nigeria.
October 11, 2015, Sunday
MORE ON TERRORISM AND: Terrorism , Refugees and Displaced Persons , Boko Haram , Chad , Nigeria
Why Is Money Still Flowing to ISIS?
Videos of Islamic State fighters with brand-name S.U.V.s make it clear that more effective efforts are needed to starve the Islamic State of revenue.
October 11, 2015, Sunday
MORE ON TERRORISM AND: Editorials , Finances , Terrorism , Syria , Treasury Department , Iraq , Islamic State in Iraq and Syria (ISIS)
Wary of Escalation in Syria, U.S. Is Waiting Out Putin's Moves
President Obama is unwilling to risk escalating the conflict and does not have a new strategy to resolve it or defeat the Islamic State.
October 9, 2015, Friday
MORE ON TERRORISM AND: Obama, Barack , Arms Trade , Assad, Bashar al- , Middle East , Defense and Military Forces , Russia , United States International Relations , Syria , Islamic State in Iraq and Syria (ISIS) , Putin, Vladimir V , Terrorism , International Relations , Free Syrian Army , Defense Department
Waves of Young Syrian Men Bring Hope, and a Test, to Europe
The exodus deprives Syria of a demographic crucial to reconstruction while threatening to create a new underclass in Europe.
October 8, 2015, Thursday
MORE ON TERRORISM AND: Middle East and Africa Migrant Crisis , Syria , Europe , Refugees and Displaced Persons , Immigration and Emigration , Terrorism
For Afghans in Kunduz, Taliban Assault Is Just the Latest Affront
For years, residents of the strategic northern province have been at the mercy of corrupt or inept government and an abusive police force.
October 8, 2015, Thursday
MORE ON TERRORISM AND: Kunduz (Afghanistan) , Afghanistan , Taliban , Ghani, Ashraf , Afghanistan War (2001-14) , Afghan National Army , Police , Terrorism
A Semantic Downgrade for U.S.-Russian Talks About Operations in Syria
The Pentagon dropped "deconfliction" from its lexicon, and rejected the notion that the United States and Russia were coordinating their side-by-side military efforts in Syria.
October 8, 2015, Thursday
MORE ON TERRORISM AND: Syria , Assad, Bashar al- , Obama, Barack , Putin, Vladimir V , United States , Russia , Carter, Ashton B , Islamic State in Iraq and Syria (ISIS) , Defense and Military Forces , United States Defense and Military Forces , United States International Relations , Terrorism , Syrian Army , Defense Department
Australian Police Suspect Terror Link
The police in Australia arrested four people in connection with the fatal shooting of a police accountant in a Sydney suburb, an attack that police have said they considered an act of terrorism.
British Police on Terrorism Conviction
Tony Mole, the detective chief superintendent of the North West Counter Terrorism Unit in England, described how a terrorist plot by a boy who is now 15 became known to the police.
Russia Shows Videos of Attacks Near ISIS
The Russian Ministry of Defense released video it says shows bombers attacking an Islamic State training camp near the town of Maadan Jedid and a command post southwest of Raqqa.
Russian Official on Airstrikes in Syria
Russia's foreign minister, Sergey V. Lavrov, said Thursday that Russia and the coalition led by the United States saw "eye to eye" on the targets of their fight against terrorism in Syria.
Obama Leads Antiterrorism Meeting
At a United Nations gathering of world leaders on Tuesday to discuss countering terrorism, President Obama said the Islamic State would be defeated.
Bomb at Yemen Mosque Kills at Least 25
The deadly suicide attack occurred on Thursday morning during prayers for the Eid al-Adha holiday in Sanaa, the country's capital, injuring dozens.
Man Held After Rotterdam Train Evacuated
The Dutch police on Friday cleared all passengers from a train traveling from Amsterdam to Paris when a man locked himself in a bathroom.
Obama Addresses Troops About 9/11
The president, speaking at Fort Meade, reflected on the 14th anniversary of the Sept. 11 terrorist attacks.
Reactions to Mumbai Bombing Convictions
Lawyers and a victim reacted after a court in India convicted 12 of the 13 men accused of bombing commuter trains in Mumbai in 2006.
The Pilots Fighting ISIS
In the year since the American air offensive against the Islamic State began, fighter pilots have assumed a huge bulk of the war effort. Here are some of the American pilots carrying out those operations.
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My Corydora catfish has lay eggs. Lots of eggs!! She spent about 18 hours on and off laying them. Problem is she now looks unwell. She is sitting very still leaning on glass side of aqurium. Could she just be tired or could she be unwell? Is there anything I can do to help her?
give her a little snack like a algea tab. but other then that make sure the water and every thing is fine then just wait and see if she is ok. thats the best i can do to help.
Thanx Ace. She is my favourite fish! My water is spot on. I will try her with something to eat and keep an eye on her. Ive turned the lights off too.
Congratulations. Cory cats are great aquarium fish.
she mite just be really tired and just needs some rest. i have not had Cory cats long and they are all ready being my second favorite there is no way they are better then my kuhli louches.
Good luck with the eggs! Hopefully you'll keep us posted om their progress.
Thanx everyone for all of your answers and support. I have 4 corydoras: 1 peppered (this is the one who has laid eggs), 1 bronze and 2 albino. I love them all but my peppered one (called Pepsi) is my favourite fish ever. She is huge compared to the others. They live very happily in my 165L aqurium with 2 bristlenosed plecs, 4 platties, 4 zebra danios, 4 rasboras, 4 gouramis and my one remaining male guppy!
Pepsi seems back to normal today. Eating everyones food!!
There are lots of eggs that survived being eaten so watch this space. I dont have a breeding tank so fingers crossed for any survivors. I have had baby platties survive in here.
I will update soon. Many thanx
This is Pepsi last year in my old smaller aqurium. How gorgeous and huge is she? x Also one of my bristlenosed plecs.
Wow! How beautiful!
Thank you, I think she is fab but I guess Im biased cos shes my baby. Yes I know Im mad!!
corys are my favorite too and she is beautiful. i have an albino.

subset: commoncrawl


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Create Extra Pantry Storage With Sponge Holders
Pantries never seem to have as much space as you need. Try sticking a sponge holder inside your pantry or cabinets for extra usable space. »
Pantries never seem to have as much space as you need. Try sticking a sponge holder inside your pantry or cabinets for extra usable space. »
A well-stocked pantry can make all the difference when it comes to making fast, healthy meals. Let's make a list of the "quick fix" ingredients that help us cook more quickly without sacrificing flavor or quality. »
You never know when you might have guests. Whether they're friends passing through or relatives just in town, impromptu entertaining doesn't have to be something to dread. If you keep a few staples on-hand all the time, you'll be ready anytime someone comes knocking that you might want to invite in for a while,… »
Walk down your supermarket's oil aisle, and you've got a surprising number of cooking oils to choose from. You don't have to stick with just one oil, though, for simplicity's sake. The Kitchn recommends keeping these three oils topped up for universal cooking and flavor needs. »
This graphic is like a cookbook in flowchart form. With it, you'll learn how to make many different, inexpensive dishes using common pantry essentials such as onions, tomatoes, and beans. »
We've talked about food storage and safety quite a bit here at Lifehacker—from what you do and don't need to refrigerate, to the best ways to stop spoiling (and wasting) food. But if you're still not sure where to store your favorite snacks, and for how long, our friends at Food52 have you covered with this complete… »
Brown sugar sitting in your pantry will eventually dry out and get clumpy, but you can keep it baking-ready with nothing by an orange peel. »
Plumbing pipes and wood boards make for great customized shelves, whether you're building a massive storage unit and built-in desk or simple bookshelves. This project shows off the potential for inexpensive pipes and wood as pantry shelves. The customization means you can neatly organize all sizes of containers and… »
If you have a little space between your fridge and the retaining wall next to it, or any space about six inches wide in your kitchen, this DIY roll-out pantry can hold a ton of canned goods and other non-perishables, roll out when you need it, and slide easily back into place when you're finished. »
If you plan your meals in advance every week, you should consider grouping the nonperishable ingredients for each meal into a storage bin—you'll not only have everything ready to go when it's time to cook but you can also quickly identify any missing items you may need to pick up. »
A pantry challenge is when you set aside a month of the year and other than basics like milk and eggs use whatever foods you already have in your pantry for meals. This practice teaches you to waste less, learn to make do, and you'll save money of the course of the month. »
If you can foods or just have a lot of foods that come in glass jars such as spaghetti sauce or pickles you've probably experienced a broken jar from accidental mishandling or even from an earthquake. Adding a 1-inch by 2-inch trim to your pantry shelf edge gives you a lip to help prevent accidental falls that you can… »
A stockpile of non-perishable foods can get you through an emergency—whether you're preparing for a hurricane, blizzard, or the zombie apocalypse. Here's a guide that outlines the best foods to stock up on for those "just in case" times of need. »
We've previously mentioned a few ways to naturally get rid of ants, but I recently found out that the majority of your pantry is suitable for warding off the pests. On top of the more common solutions of mint and bay leaves, it turns out you can use a variety of other spices and extracts as well. »
One of the authors at Ikea modification weblog Ikea Hackers had a 10" gap between their refrigerator and the wall. The author's house didn't have a pantry so he added casters to Ikea Kilby Bookshelves, added a bit of moulding to cover the casters, painted the side of the shelf to match his cabinetry, and added a… »
If you buy spaghetti, linguine, or other long pastas in bulk it can be difficult to find a good way to store the excess once you open the large package. Tall thin Pringles brand potato chip cans excel at this use. »
Another one for the "why didn't I think of that" files: Turn a magazine holder on its side and stick two pins in it for space-saving organization in the pantry. »
If your goal for the holidays or new year is a healthier you, those cabinets full of sugary snacks and salty, over-processed junk food are your first serious roadblock. It's time to throw out your junk food in a dramatic blaze of glory so you'll stick to your convictions. Here's a step-by-step guide to help you clean… »


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Posted by Geoff on 2006-02-23
I've been to Jack's a bunch of times and I am completely impressed with their bar food. I especially like their chicken pot pie, turkey burger and shimp w/ gorganzola pizza. The 404 wine bar next door is a cool space too.


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Lizzy Yarnold led a triumphant Team GB back to the United Kingdom after capping a superb skeleton season with a gold medal at the Winter Olympics in Sochi.
Yarnold was in a class of her own over the four runs at the Sanki Sliding Centre, finishing almost a second ahead of closest rival Noelle Pikus-Pace.
The World Cup champion broke the track record with her first run, and her lead was barely threatened as she emulated the success of Amy Williams in Vancouver.
"This year on the World Cup circuit I put in some great performances," said Yarnold at the Team GB press conference at Heathrow. "I think I was on the podium seven times in eight races and I was learning lessons the whole way.
"Once I heard I was selected for Sochi, of course it was my dream to do my best and do myself justice.
"Coming into the skeleton competition, I was so nervous because I had done everything that I could. Anything left was out of my control.
"Once the competition started, the track record with my first run was a complete surprise. I think I was just unbelievably focused on every single corner and relaxed, I felt connected with the sled.
"Looking back on the whole thing, I still can't believe how it went so well. I'm just so pleased with it all."
Team GB Chef de Mission Mike Hay admitted he was "proud and privileged" of the team, and he insisted the record medal-count would have been higher had the likes of Elise Christie and Jack Whelbourne not endured such misfortune in the short track speed skating competiton.
"Our athletes were well prepared and performed wonderfully well in very tough conditions," Hay said.
"Jenny Jones started the ball rolling with a fantastic bronze medal. Lizzy Yarnold was completely dominant in winning her gold medal, and to have two teams winning medals in curling was a thrilling moment.
"Some of us are disappointed that we may have left a couple of medals out there in Sochi. Our short-track speedskaters did fantastically well and were extremely unlucky not to come back with a medal.
"I'm very proud of the team. I'm not sure about the relevance of comparing with 1924, but we are delighted to have exceeded our target and come back a successful delegation.
"In performance sport you are measured by the medal table and how well you did, and I'm delighted to say our athletes did Great Britain proud."
Jones collected the team's first medal with a memorable performance in the inaugural Olympic slopestyle snowboarding event, which she almost missed after suffering concussion in a bad fall before Christmas.
"In December I had quite a bad concussion, but I had a lot of support from the team physios and that was great," she said. "But I didn't expect to be heading to the Olympics and it was very touch and go.
"But I made it and it was a great experience to showcase slopestyle snowboarding to the world. I think it's been a great success, a lot of people have been excited by the sport - and I got a medal!"
Eve Muirhead skippered Team GB to bronze in the women's curling and revealed the team were spurred on by a disappointing performance in Vancouver, where they won only three of their nine matches.
"Competing at Vancouver couldn't have helped me enough," she said. "We were very disappointed and it took a long time to get over it.
"But I put together this new team, and I knew from the start these girls were 100 per cent focused on the same goal as myself and they gave everything.
"Over the last few years we've had several grand slam wins, and European title and a world title so we knew we were one of the favourites in Sochi.
"We were gutted to lose the semi-final and it took a long time to get over it. But we had to come back the next morning for the bronze medal match.
"Overall, I think the experience from Vancouver really helped. To come away and finish on a high with a bronze medal really proved that our hard work over the years has paid off."
Who is your sportswoman of the month?
Lizzy Yarnold
Jenny Jones
Eve Muirhead
Charlotte Edwards
Arran Brindle


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O2 has been the official sponsor for the England Rugby Team since 1995. 'Brands do need to consider the teams or individuals they sponsor and how that will affect the overall brands reputation. Rugby is renowned for the traditional values it stands for so if O2 are looking to still remain strongly linked to sports industry then the upcoming 2011 Rugby World Cup is certainly a great opportunity'
Carolina Consulting Ltd review brands reputations and the brand perception by consumers regularly. EDF Energy is a brand that has a huge focus on increased brand reputation over the past 12 months; they are the official energy sponsors for the 2012 Olympic Games and sponsor the a famous London tourist attraction, The London Eye.
Carolina Consulting Ltd have close links with the energy industries and while monitoring some of the changes in brand reputation over the last year, EDF Energy sponsorship deals can be linked directly to their increase in customer acquisition and brand awareness. 'A customer's ability to evoke and recognize a brand is of the upmost importance. Brands build trust. If a customer associates a brand with a sponsorship deal they have confidence in they are far more likely to purchase or recommend that service, being associated with sporting events like the 2012 Olympics or the Rugby World Cup are certainly a great way to get recognised' adds Sara King Cote of Carolina Consulting Ltd.
Carolina Consulting Ltd has recognized many top brands shift their sponsorship strategies to align with their model brand reputation.
# # #
Carolina Consulting Ltd entered the London market in 2008. We are an innovative consulting company that opened its doors in response to the demands from clients for a more effective, personal and results driven marketing approach.


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To establish reversibility of cardiac phenotypes in hypertrophic cardiomyopathy (HCM) by generating bigenic mice in which expression of the mutant transgene could be turned on and off as needed.
Advances in molecular therapeutics could ultimately lead to therapies aimed at correcting the causal mutations. However, whether cardiac phenotypes, once established, are permanent, or could be reversed, if expression of the mutant protein is turned off, is unknown.
We generated ligand-inducible bigenic mice, turned on and off expression of cardiac troponin T-Q92 (cTnT-Q92), responsible for human HCM, and characterized molecular, histological, and functional phenotypes.
We established 6 lines and in dose-titration studies showed treatment with 1000 ug/kg of mifepristone consistently switched on cTnT-Q92 expression in the heart. 16 days of expression enhanced myocardial systolic function without changing myocardial cAMP levels. Levels of PTEN, a regulator of cardiac function; phospho-PKC-Zλ-Thr538 and phosphor-PKD-Ser744-748 were reduced, while mRNA levels of NPPA, NPPB (hypertrophic markers), and ATP2A2 and procollagen COL1A1, COL1A2, and COL3A1 were unchanged. 70 days of expression increased COL1A1 and COL1A3 mRNAs levels and collagen volume fraction and reduced levels of NPPA and NPPB. Switching off expression of the cTnT-Q92 reversed the molecular and histological phenotypes completely.
The initial phenotype induced by cTnT-Q92 is enhanced myocardial systolic function followed by changes in signaling kinases and interstitial fibrosis. Established phenotypes in HCM are reversible if expression of the mutant protein is turned off. These findings provoke pursuing specific therapies directed at correcting the underlying the genetic defect in HCM.
Human hypertrophic cardiomyopathy (HCM) is a genetic disease characterized by unexplained cardiac hypertrophy, myocyte disarray, interstitial fibrosis and increased left ventricular ejection fraction (LVEF) (1-3). Mutations in at least 10 different sarcomeric proteins cause HCM (2) and those in cardiac troponin T (cTnT) account for approximately 15 to 20% of HCM cases (2). HCM due to cTnT mutations is generally characterized by an increased LVEF, minimal or mild hypertrophy, severe disarray, fibrosis and a high incidence of sudden cardiac death (SCD) (4;5).
Elucidation of the molecular genetic basis of HCM has shifted the focus toward elucidation of the pathogenesis of HCM and developing therapies aimed at reversing or attenuating the evolving phenotypes and ultimately correcting the primary genetic defects. Currently, it is unknown whether turning off expression of the mutant protein could lead to reversal of the established phenotypes; or the phenotypes, once established, are permanent. Proving the reversibility of the HCM phenotypes is fundamental and pre-requisite to pursuing specific molecular therapies directed at correcting the underlying genetic mutation in HCM. Thus, to determine the reversibility of the evolving phenotypes in HCM and gain insight into the pathogenesis of cardiac phenotypes, we generated an inducible bigenic mouse model in which expression of the cTnT-Q92, known to cause HCM in humans (4), could be switched on and off as needed. The inducible bigenic model is based on ligand-dependent activation of an inactive tripartite regulatory protein containing the ligand-binding domain (LBD) of a truncated human progesterone receptor (PR) that upon activation with mifepristone binds to the DNA sequences in the target transgene and induces expression of the target gene (6).
The Animal Subjects Committee of Baylor College of Medicine approved the experiments. The regulator is a tripartite protein comprised of the LBD of a C-terminal truncated (deletion of 42-amino acid) human PR, DNA-binding domain of yeast GAL4 protein and the transcription activation domain of the p65 subunit of human NFκB transcription factor. It is inactive in its native state because the modified LBD lacks the ability to respond to progestins but responds to synthetic anti-progeston mifepristone (RU486) (7). The target transgene is comprised of 4 tandomly placed 17-bp (CGGAGTACTGTCCTCCG) GAL4 upstream activating sequences (UAS) and a TATA box positioned 5' to a mutant cTnT-Q92 cDNA. Expression of the target transgene could be turned on and off by adding or withdrawing mifepristone. The α-myosin heavy chain (α-MyHC) promoter affords cardiac-restricted expression of the regulator protein and consequently, that of the cTnT-Q92 (Figure 1A).
The α-MyHC promoter (clone 26) was a kind gift from Dr. Jeffery Robbins (University of Cincinnati) and the PAP-CMV-GLp65-SV and p17x4-TATA-CAT plasmids were generous gifts from Dr. Sophia Y Tsai (Baylor College of Medicine). To clone the regulator transgene, the GLp65 fragment containing the coding sequences for the tripartite regulator protein was excised from PAP-CMV-GLp65-SV at the unique Asp718 and BamH1 restriction sites, blunt ended and ligated to the blunt-ended SalI and HindIII cloning sites of clone 26, downstream to the α-MyHC promoter (Figure 1A). To clone the target transgene, a previously described full-length (1.1 kb) human cTnT-Q92 cDNA (8) was released from the pSP73/cTnT-Q92 clone and inserted at XhoI and KpnI sites, replacing the CAT sequences in the p17x4-TATA-CAT clone. The correct orientations and the sequence of the transgenes were verified by direct sequencing. The regulator transgene (~7 kb) was released from the vector by digesting the clone at the encompassing Not1 sites and the target transgene by digesting the clone at HindIII and KpnI sites (~2 kb). Transgenes were purified from the gel and injected into fertilized mouse zygotes (FvB/N) and the pups were screened by PCR for the regulator and target transgenes and for the β casein, as an internal control.
Mifepristone (BIOMOL, PA) was initially dissolved in dimehtyl sulfoxide (DMSO) at a concentration of 40 mg/ml and the working solution was prepared in sesame oil at a concentration of 1 mg/ml. To induce target gene expression, dose-titration and time-course experiments were performed and mifepristone was injected intra-peritoneally (n= 5 to 12 adult mice per group) at 3 dosages of 300, 500 and 1000 μg/Kg/day for 2, 4, 8 or 16 days (short-term) or 70 days (long-term). To switch off expression of the transgene, mifepristone was discontinued for at least 16 days, which is longer than 5 half-life of cTnT in the heart (9) and for 70 days in long-term experiments. Parallel experiments were performed in non-transgenic (NTG) injected with mifepristone and in bigenic mice injected with a placebo (sesame oil). Expression of the target transgene was determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunoblotting after switching on or off expression of the transgene at the above time points. Short-term (2, 4, 8, and 16 days) induction of expression of cTnT-Q92 transgene was achieved through daily intra-peritoneal injections. To induce long-term expression of cTnT-Q92 transgene, mifepristone controlled-released pellets (Innovative research of America, Sarasota, FL) were prepared to deliver 1000 μg/Kg per day and implanted subcutaneously.
RT-PCR and immunoblotting were performed using transgene-specific oligonucleotide primers and monoclonal anti-troponin T antibody 2D10 (Research Diagnostic, MD), respectively, as described (8). Relative expression levels of the transgene and endogenous cTnT mRNAs was determined by RT-PCR using primers (forward 5'TTCATGCCCAACTTGGTGCC and reverse 5'CTCTCTTCAGACAGGCGGTTC) to amplify a 260 bp of endogenous and transgene cTnT mRNAs followed by digestion with 10 units of BstU1 restriction enzyme, which digests the endogenous (mouse) but not the transgene (human) cTnT into 2 fragments of 184 and 76 bp.
Expression levels of molecular markers of cardiac hypertrophy, namely A-type natriuretic peptide (NPPA), B-type natriuretic peptide (NPPB), skeletal α-actin (ACTA1) and sarcoplasmic reticulum calcium ATPase 2a (SERCA2a or ATP2A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as well as expression levels of procollagen COL1(α1), COL1(α2) and COL3(α1) mRNAs (COL1A1, COL1A2 and COL3A1, respectively) were determined by real-time quantitative RT-PCR using specific Taqman probes and primers in an 7900HT SDS unit (Applied Biosystem, Inc).
Expression levels of selected phosphorylated and total signaling kinases, implicated in affecting cardiac structure and function, were detected using 20 μg of cardiac protein extracts and phospho-specific and pan-specific antibodies, as described (10). Specific information about antibodies is available upon request.
Echocardiography was performed prior to and following switching on and off expression of the transgene at each dose and time point. Mice were anesthetized with intra-peritoneal injection of anesthetics (avertin 0.2 ml /10 gram body weight, intraperitoneal injection of a 1.25% solution in the long-term experiments) and trans-thoracic echocardiography was performed using an Acuson Sequoia Cardiac System equipped with 15-MHz linear transducer (Acuson Co., Mountain View, CA) per conventional methods (11). Echocardiographic parameters were measured in at least 3 consecutive cardiac cycles by an investigator totally unaware of the genotypes or the treatment groups.
The extent of cardiac myocyte disarray and interstitial fibrosis were quantified as published (10;12). Percentage of myocardium showing disarray was determined in a semi-quantitative manner in 12 fields per section and 10 sections per mouse. Similarly, collagen volume fraction (CVF) was calculated using quantitative automated planimetry in 12 fields per section and 10 sections per mouse. Myocyte cross sectional area (CSA) was determined by quantitative indirect immunofluorescence in approximately 2500 myocyte per group, as published (10;12). All histological quantifications were performed by an examiner who had no knowledge of group assignment.
Since cAMP is a major mediator of myocardial contractile function, we measured total myocardial cAMP levels by enzyme immunoassay per instruction of the manufacturer (Amersham Biosciences, UK). In brief, 100 mg of ventricular myocardium (n=4 to 8 mouse per group) was homogenized in Hank's balanced salt solution with 5 mM EDTA without calcium and magnesium and centrifuged at 1000 g at 4°C for 15 minutes. The homogenates were subjected to ion-exchange chromatography using columns containing anion exchange silica sorbents followed by analyte elution with acidified methanol. The eluted samples were added to an assay buffer containing rabbit anti-cAMP antibody in 0.05 M acetate, 0.02% (w/v) bovine serum albumin and mixed thoroughly. Then, an equal volume of cAMP-horseradish peroxidase conjugate in 0.05 M acetate buffer, pH 5.8 was added to each reaction and washed with 0.01 M phosphate buffer pH 7.5 containing 0.05% (v/v) tween 20. The mixtures were added to microplates coated with a donkey anti-rabbit IgG antibody, incubated at 4°C for 60 minutes. Following washing, the enzyme substrate was added to the wells and the reactions were terminated after 1 hour of incubation at room temperature with 1.0 M sulfuric acid and the optical density was determined immediately in a plate reader at 450 nm.
Differences between the baseline and follow up values in each group were compared by paired t-test. Differences in variables among NTG, placebo, and mifepristone groups were compared by ANOVA, followed by Bartlett's test to determine the homogeneity of the variances. Variables with unequal SD were compared by the non-parametric Kurskall-Wallis test.
Three lines of regulator (α-MyHC/GLp65-GAL4-ΔPR) and 7 lines of target (4xGAL4UAS-TATA-cTnT-Q92) transgenic mice were generated. The target transgenic lines were tested for background expression of the cTnT-Q92, after confirmation of the absence of background leak, mated to the regulator transgenic mice and 6 lines of bigenic mice were established. Monogenic and bigenic mice were identified by PCR using transgene-specific oligonucleotide primers (Figure 1B).
Expression of the transgene mRNA and protein was detected consistently after daily administration of mifepristone at 1000 μg/Kg/day for 16 days. Shorter durations or lower doses of administration of mifepristone did not consistently induce expression of the transgene. The results of RT-PCR (in three lines, Figure IC) and immunoblotting (in one line, Figure 1C) confirmed expression of the cTnT-Q92 mRNA and protein, respectively. No significant cTnT-Q92 expression was detected in bigenic mice injected with a placebo or in the target monogenic mice. The relative expression levels of the induced transgene cTnT was low in all lines and comprised < 10 % of the total cTnT mRNA in the heart. Transgene protein and mRNA was not detected 16 days after discontinuation of mifepristone (Figure 1D).
In the long-term study, 2/9 bigenic mice died 3 and 9 days after mifepritstone injection, in contrast to 0/11 NTG mice injected with mifepristone or 0/9 bigenic mice injected with the placebo. Baseline echocardiographic indices were unremarkable in the two mice died after induced gene expression. Since these mice were found dead, no histological or molecular studies were performed.
Echocardiographic indices of myocardial function were compared at the baseline prior to injection of mifepristone; 16 and 70 days after induction of expression of the cTnT-Q92 in the heart; and 16 days and 70 days after discontinuation of mifepristone administration. The results were remarkable for a smaller left ventricular (LV) end systolic diameter (ESD), increased LV fractional shortening (FS), LVEF, velocity of circumferential fiber shortening (Vcf) and maximum aortic flow velocity in induced state at both time points (16 and 70 days) as compared to the baseline values. There was no significant difference in the LV wall thickness, end diastolic diameter (EDD), or mitral inflow early (E), late (A) and E/A. However, deceleration time (DT) of E velocity was prolonged in the bigenic mice injected with mifepristone. Table 1 shows the echocardiographic data at the baseline, 16 days after induced gene expression and 16 days after discontinuation of mifepristone injection (data for 70 days induced expression were largely similar, except the mean heart rate was 462 ± 71 bpm, and are not shown). Discontinuation of mifepristone led to reversal of the echocardiographic phenotypes. There were no significant changes in other echocardiographic indices. Injection of mifepristone, at 1000 μg/Kg/day, to NTG mice alone had no significant effects on LV systolic function or other echocardiographic indices (Table 1).
To determine whether increased cAMP levels was responsible for the enhanced myocardial systolic function, we measured total myocardial cAMP levels in the experimental groups. There were no significant differences in myocardial cAMP levels among the experimental groups (NTG: 15.9 ± 3.9; target transgenic: 17.5 ± 3.0; regulator transgenic: 17.0 ± 3.3; bigenic placebo: 16.3 ± 3.4; bigenic mifepristone on: 13.5 ± 4.8; bigenic mifepristone off: 13.1 ± 1.5 fmol/gram, p=0.162).
To determine whether induced expression of cTnT-Q92 also led to changes in expression of activated signaling molecules, we assayed for levels of selected signaling kinases, implicated in cardiac function, by immunoblotting using phospho-specific antibodies. The results, partially shown in Figure 2, were notable for a significant reduction in the expression level of PTEN and to a lesser extent, in levels of p-PKC-Zλ-Threonine 538 and p-PKD-Serine 744-748, when transgene was turned on for 16-day. There were no significant changes in levels of PI3K-p110-α, β, δ; and p85 or p-PKC-δ-Serine 643, p-PKA, p42/p44 MAPKs, GSK3β or calcineurin. Discontinuation of mifepristone led to normalization of the activated signaling molecules. There were no statistically significant changes in the expression levels of selected signaling kinases following shorter (2,4 and 8 days) or longer (70 days) duration of induction of expression of cTnT-Q92 in the heart. To determine, whether changes in the expression levels of signaling kinases was independent of administration of mifepristone, NTG mice were injected with 1000 μg/Kg/day of mifepristone (n=4) and immunoblotting was repeated. Mifepristone injection alone did not induce significant changes in levels of p-PKC-Zλ-Threonine 538 and p-PKD Serine 744-748 or PTEN in the heart.
Expression levels of NPPA, NPPB, ACTA1, ATP2A2, COL1A1, COL1A2 and COL3A1 were determined using real-time quantitative RT-PCR 16 and 70 days after induction of expression of cTnT-Q92 and after switching off expression of the transgene. After 16 days of induced expression of cTnT-Q92, myocardial levels of molecular markers of hypertrophy and fibrosis were largely unchanged with the exception of a modest decrease in ATP2A2 levels (data not shown). However, long-term (70 days) induced expression of cTnT-Q92 led to significant reductions in expression levels of NPPA and NPPB without a significant change in ATP2A2 levels (Figure 3A). Furthermore, expression levels of COL1A1 and COL1A3, encoding the predominant cardiac collagen, were increased significantly, while expression level of COL1A2 was largely unchanged (Figure 3A).
To detect whether induction of expression of cTnT-Q92 was associated with morphological or histological phenotypes characteristics of human HCM, we determined heart weight, heart weight/body weight ratio, LV weight/body weight ratio, myocyte CSA, number of myocytes per microscopic fields, % CVF and the extent of myocyte disarray. Short-term induced expression of the cTnT-Q92 did not lead to discernible changes in histological or morphological phenotypes, since no significant differences in morphometric histoligical indices were detected among NTG, NTG injected with mifepristone, target and regulator monogenic and bigenic mice in the placebo and mifepristone groups (Table 1). However, as shown in Figure 3B, induced expression of cTnT-Q92 for 70 days led to 2.1 fold increase in CVF, in accord with the observed increased expression of the procollagen mRNAs. Turning off expression of cTnT-Q92 led to normalization of CVF (NTG: 2.5 ± 0.66; switch on: 4.2 ± 1.0; switch off: 2.0 ± 0.24, p=0.007). With regard to indices of hypertrophy, neither heart weight/body weight ratio, nor echocardiographic indices, nor myocyte CSA was significant different among the experimental groups. Myocyte disarray comprised 6.3 ± 3.6 % of the myocardium in the bigenic mice induced with mifepristone for 70 days, while it was 2.8 ± 2.6 % in NTG mice (T=2.02, p=0.08) and 5.0 ± 4.7 % in bigenic mice following turning off expression of the transgene (F= 1.05, p=0.373 among the three groups).
We describe a switch on – switch off bigenic mouse model in which expression of the mutant sarcomeric protein cTnT-Q92, responsible for human HCM (4), was turned on and off by administering and withdrawing mifepristone, the activating ligand for the otherwise inactive regulatory protein. Short-term induced expression of the mutant cTnT-Q92 enhanced myocardial systolic function, a characteristics phenotype of HCM, independent of myocardial cAMP levels and in the absence of histological or molecular phenotypes of hypertrophy, disarray or fibrosis. Enhanced myocardial contractile performance was associated with reduced levels of selected active signaling kinases, implicated in regulating cardiac function. The long-term induced expression of cTnT-Q92 led to increased expression of procollagen genes and interstitial fibrosis, while levels of the molecular markers of hypertrophy were reduced. The induced phenotypes, namely, interstitial fibrosis, increased expression of procollagen genes and signaling kinases and enhanced cardiac systolic function were completely reversed upon switching off expression of cTnT-Q92. These results, in a genetic animal model of human HCM mutation, establishes the reversibility of HCM phenotypes, a pre-requisite in designing future therapies targeted at correcting the underlying genetic mutations. The results also identify the primary defect conferred by cTnT-Q92 as enhanced myocardial systolic function, independent of myocardial cAMP levels. Thus, the potential utility of the switch on-switch off bigenic mouse model are at least two-fold: First, to establish the reversibility of the cardiac phenotypes induced by HCM mutations; and second, to identify the initial defects conferred by the mutations followed by delineation of the sequence and pathogenesis of ensuing functional, molecular, histological and morphological phenotypes in HCM.
The finding of reversal and normalization of interstitial fibrosis following switching off expression of the mutant troponin T was confirmed by two complementary methods of real time RT-PCR analysis of expression of major procollagen genes in the heart as well as by quantitative morphometric analysis of collagen protein in myocardial sections stained with collagen-specific dye. Reversal and normalization of interstitial fibrosis in the heart has potential clinical implications, since interstitial fibrosis is a potential risk factor of SCD in humans with HCM (13). In contrast to interstitial fibrosis, however, cardiac hypertrophy was absent in the bigenic cTnT-Q92 mice, as was also the case in the conventional transgenic cTnT-Q92 mice (8;14). Notably, humans with HCM due to cTnT-Q92 usually exhibit mild or minimal hypertrophy (15). The absence of discernible hypertrophy in the bigenic mice or even the presence of smaller myocyte and heart size in the conventional cTnT-Q92 mice (11;14) could simply reflect the enhanced cardiac performance, which affords the heart the ability to handle the normal load at a much lesser cardiac mass. In accord with the above, expression levels of markers of cardiac mass, namely NPPA and NPPB, were reduced significantly following long-term induced expression of cTnT-Q92. Regarding myocyte disarray, it was increased modestly in the bigenic mice followed long-term induced expression. However, changes were of borderline statistical significance. Therefore, it remains to be determined whether switching off expression of the mutant protein could also lead to reversal of hypertrophy and disarray, as we have shown previously through pharmacological interventions in genetically engineered animal models (10;12).
The molecular basis of enhanced myocardial contractile performance following induction of expression of cTnT-Q92 was not discerned, suffice that it was independent of myocardial cAMP levels or injection of mifepristone. Previous studies have shown that myofibrils isolated from the hearts of the conventional cTnT-Q92 transgenic mice exhibit enhanced calcium sensitivity of tension generation, evidenced by a higher values of half-maximally activating –log of the molar free Ca+2 concentration (pCa50) (16). The latter in conjunction with the results of studies in skinned fibers isolated from rabbit and pig hearts showing enhanced Ca+2 sensitivity (17;18), is likely to explain the observed enhanced cardiac systolic function in our bigenic mice in the induced state. In addition to enhanced Ca+2 sensitivity; other mechanisms, including changes in signaling molecules, as observed following induced expression of cTnT-Q92, could also affect cardiac function. Regarding the potential impact of signaling molecules, our study was limited to analysis of a limited number of signaling molecules, implicated in regulating cardiac structure and function, and was not comprehensive. Nonetheless, a significant change in the expression of tumor suppressor PTEN, implicated in regulating cardiac function (19), was detected. PTEN, in addition to affecting cardiac function, also plays an essential role in proper cell adhesion, alignment and migration (20;21). Thus, given the essential role of PTEN in cellular attachment and alignment, reduced expression of PTEN could be involved in subsequent evolution of myocyte disarray in HCM. The molecular mechanisms that regulate expression and activation of PTEN, p-PKC-Zλ-Threonine 538 and p-PKD-Serine 744-748 in the heart remain unknown. We showed reduction in the expression levels of selected signaling molecules was independent of mifepristone administration. The role of changes in the activation state of selected signaling kinases, noted above, in the pathogenesis of cardiac phenotypes in HCM merits additional investigations.
The findings of the present study are restricted to the cTnT-Q92 mutation. We note that the initial impetus and the ensuing early and intermediary phenotypes, imparted by the mutant sarcomeric proteins are likely to be diverse and vary according to the topography of the causal mutations and genes. The bulk of experimental data implicate enhanced myofibrillar Ca+2 sensitivity as the initial defect imparted by mutations in the cTnT (16-18). However, with regard to mutations in the β-MyHC and other genes, reduced myofibrillar and myocardial contractile performance and/or reduced ATPase activity have emerged as the prime candidates as the initial defects (reviewed in (2)). The complexity of the pathogenesis of HCM phenotypes is further elucidated by the divergence of phenotypic expression of mutations in the same causal gene, which lead to morphologically contrasting phenotypes of hypertrophic, dilated and restrictive cardiomyopathies (22;23). Therefore, a single animal model is insufficient to elucidate the pathogenesis of HCM caused by a diverse array of mutations. It is also noteworthy that there are several differences between transgene (human) and endogenous (mouse) cTnT sequences that could confound the effect of the mutation. Our previous studies, however, have shown that these differences do not induce discernible histological or echocardiographic phenotypes (8). Hence, the observed phenotypes in the switch on model likely reflect the effect of the mutation.
In conclusions, we describe a switch on-switch off bigenic mouse model in which expression of cTnT-Q92, responsible for human HCM, could be turned on and off as needed. The results show that the established cardiac phenotypes in HCM are not permanent and could be reversed upon turning off expression of the mutant protein. The findings also indicate that the initial phenotype induced by the cTnT-Q92 is enhanced myocardial systolic function followed by changes in activation of signaling molecules implicated in cell organization and myocyte function followed by interstitial fibrosis. These findings provoke pursuing specific therapies directed at correcting the underlying the genetic defect in HCM.
Supported by grants from the National Heart, Lung, and Blood Institute, Specialized Centers of Research P50-HL54313, RO1 HL68884, and a TexGen grant from Greater Houston Community Foundation.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's Canada Institute for Scientific and Technical Information in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Figure 2.
Comparison between rmVO2 values of legs of MS population ranked according to mobility. Values of healthy controls were also included. Legend to figure: rmVO2, resting muscle oxygen consumption; MS, Multiple Sclerosis; HC, healthy controls. Statistical analysis: Unpaired Student T-test between MS low and high mobility; One-way ANOVA among MS low and high mobility, and HC.
Malagoni et al. BMC Neurology 2013 13:52 doi:10.1186/1471-2377-13-52


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SACRAMENTO -- The Sacramento Kings needed a victory in the worst way and used their best performance of the season to get it. The Kings used a strong third quarter and solid fourth period to run away from the Los Angeles Lakers 113-97 Wednesday night at Sleep Train Arena. The Kings (3-8) scored a season-high number of points and also did a tremendous job defensively against Lakers' big men, power forward Pau Gasol and center Dwight Howard, to help slow Los Angeles (6-6). The Kings received strong offensive contributions with six players reaching double figures, led by Marcus Thornton's 23 off the bench. Defensively, the Kings were active and swarmed Howard, who entered the game averaging 20.3 points a game, but attempted just four shots. "(The Kings) did a good job of never leaving me and never letting me get to the spots I like," said Howard, who scored seven points. Lakers guard Kobe Bryant said the Kings did a good job of "sitting in Howard's lap." "When teams have do...
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New Horror Film "Kill Game" Screening at Cannes Shot on Blackmagic Cinema Camera
Fremont, CA - May 19, 2014 - Blackmagic Design today announced that "Kill Game," a new suspense thriller/horror film produced by Marty Murray of Full Throttle Pictures and distributed by Vantage Media International (VMI), and being screened at the upcoming Cannes Film Festival, was shot entirely on the Blackmagic Cinema Camera EF and graded and edited using DaVinci Resolve.
The filmmakers were so pleased with the results gained on "Kill Game", they decided to also use the Blackmagic Cinema Camera for all reshoots and additional photography for their other new film, "PARANORMAL ISLAND", starring Lance Henriksen and Briana Evigan. Ari Golan, owner of Atomic Imaging and a noted cinematographer, shot all of the steadicam/B-Cam, underwater and 2nd Unit footage for "Kill Game," as well as shooting all the second unit, pick ups and underwater sequences for "PARANORMAL ISLAND," after he returned from working with Peter Jackson on Hobbit 2.
"Kill Game" is an intense new horror film from Full Throttle Pictures and Atomic Imaging, who supplied all three of the Blackmagic camera packages. The film was directed by Robert Mearns, with cinematography by Andrew Strahorn. The film follows a group of high school pranksters who one day go too far and kill another student. Years later they return to their home town when one of their friends is mysteriously found dead and one by one each member of the group is slowly and methodically murdered.
To capture the extreme brutality and dark imagery of Mearns's vision, Producer Marty Murray and Co-Producer Ari Golan approached Strahorn and chose to use multiple Blackmagic Cinema Cameras as the main cameras on the shoot.
"The director had a very specific vision for how he wanted every shot to look, and he wanted the entire movie to capture the darkness and horror that this group of friends was facing. We needed dark skies and morbid killing scenes. The brutality of the violence had to come out, but not be overwhelming. To get this, we needed a camera with high dynamic range and the ability to shoot in RAW so that the director could get all the data possible to really exercise his creativity," said Marty. "The Blackmagic Cinema Camera fit everything we needed and the images were incredible."
The film was shot in RAW using two Blackmagic Cinema Cameras. Footage was brought into DaVinci Resolve for a first pass grade and was then transferred to ProRes for editing. Once the footage received an initial edit, it was exported back to DaVinci Resolve for final color correction, final editing and audio fixes by Ari Golan It was then output to ProResHQ, BluRay and DVD for final delivery to Cannes.
"We really wanted to shoot on the Blackmagic cameras because of their workflow and image quality. Also, The camera had to be tough enough to work in some very difficult situations, and the Blackmagic cameras were extremely flexible. The entire workflow was simple with RAW and DaVinci Resolve. We really put the camera through some torture tests and the image quality was great throughout," said Ari.
The shoot included working in a number of difficult locations and extreme conditions, such as shooting in the worst heat wave of 2013 in the US. In order to meet his vision, the director needed the action scene images to be up close and have a truly visceral feel. The Blackmagic Cinema Cameras allowed the filmmakers to get right into the middle of the action with their small form factor and design.
"It is a tough camera and held up great. One of the toughest shots we had was a POV shot from inside a car. We wedged ourselves into the trunk with no problem. The camera's small form factor let me get my shot without worrying about losing image quality," said Ari. "For another shot, we needed to shoot underwater and had a leak in the waterproof camera housing. About a quarter inch of the camera was sitting in water. It was not a problem at all, and the footage came out great. Although we certainly don't suggest the camera is waterproof, nor recommend testing that attribute, it did survive the unintended water torture test."
"We will always shoot with the Blackmagic cameras from now on. They give us the best image quality per dollar, and allow us to do multi camera shoots. They really freed us up creatively to meet the director's vision on 'Kill Game' and they gave us outstanding cinematic images at an amazing value," said Ari.
The film will be shown at the Cannes film market event, Le Marche du Film, in May. It will also be shown at the 2014 Toronto Film Festival and the upcoming international Shriekfest horror film festival, and will be distributed to theaters later in 2014 by VMI.
Press Photography
Product photos of Blackmagic Cinema Camera and DaVinci Resolve are available at www.blackmagicdesign.com/press/images.
About Blackmagic Design
Blackmagic Design creates the world's highest quality video editing products, digital film cameras, color correctors, video converters, video monitoring, routers, live production switchers, disk recorders, waveform monitors and film restoration software for the feature film, post production and television broadcast industries. Blackmagic Design's DeckLink capture cards launched a revolution in quality and affordability, while the company's Emmy™ award winning DaVinci color correction products have dominated the television and film industry since 1984. Blackmagic Design continues ground breaking innovations including stereoscopic 3D and 4K workflows. Founded by world leading post production editors and engineers, Blackmagic Design has offices in the USA, United Kingdom, Japan, Singapore, and Australia. For more information, please check www.blackmagicdesign.com.News Source : New Horror Film "Kill Game" Screening at Cannes Shot on Blackmagic Cinema Camera
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- DaVinci Resolve Studio Used by Trish Cahill at Soundfirm to Grade 'The Dressmaker'
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- Fuji TV's "Busu to Yajyu" Uses Blackmagic Cinema Camera and Pocket Cinema Camera
- McDonalds Spot Shot on URSA and Edited and Graded in Resolve 12 by O2 Filmes
- Blackmagic Design Announces Major MultiView Update
- Blackmagic Design Announces DaVinci Resolve 12 is Now Shipping
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Angling – The Most Popular 'Gateway' Activity in the US
The 2012 Outdoor Recreation Participation Report indicated that 46.2 million Americans fished in 2012. ... Full story
Fishing Foundation Hopes to Hook Hispanics
A national boating and fishing organization is launching a multimillion-dollar campaign next year aimed at getting more Hispanics involved in fishing. ... Full story
IFTD and ICAST Show 2013
The 2013 IFTD and ICAST show in Las Vegas was considered to be a huge success by all parties. ... Full story
Take Me Fishing!
The Recreational Boating and Fishing Foundation (RBFF) has launched a national campaign called Take Me Fishing. ... Full story
Three Rivers Fly Fishing Carp Cup
Three Rivers Angler will be hosting their annual Fly Fishing Carp Cup contest on Saturday August 17 from 8:00 a.m. to 6:00 p.m. to benefit the Legacy Parks Foundation. ... Full story
Hardy North American – New Upper Midwest Appointment
Jim Murphy, President of Hardy North American, has announced the appointment of a new Representative for the Upper Midwest. ... Full story
Why Not Try Fly Fishing for Carp?
For years carp have been dubbed a 'trash fish' and snubbed by anglers but have they been getting a bad rap? ... Full story
Team USA Dominate World Youth Fly Fishing Championships
The 12th FIPS-Mouche World Youth Fly Fishing Championships saw Team USA bringing home two medals for the third year in a row. ... Full story
Custom Fly Manufacturer DryFlyOnline Launches Online Retail Site
DryFlyOnline has launched a new website offering anglers direct access to custom-tied flies. ... Full story
IFTD and ICAST to Co-locate Again in 2014
'Angling International' report that following a successful show in Vegas, 2014 will see another agreement between the two organisations. ... Full story
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Campaign to Stop the Pebble Mine in Alaska's Bristol Bay Tops Orvis Customer Matching Grants for 2013A national campaign to stop the controversial Pebble Mine in...
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Vermont BANS Felt Soled Wadding BootsThe use of felt-soled waders or boots in Vermont waters...
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Fly Fishing The World Visits IcelandThere is an Atlantic Salmon Iceland special version of the...
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I was looking for Halal restaurant till I found this .
I like the food and the atmosphere there , hard to find a table at lunch time .
A wide variety of African dishes plus good location near Kenyatta avenue and the city center .
Prices are reasonable .
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text: Gold Plated 'Surprise Me' Necklace by EVJ £250$541.75€369.97$411.30 In stock £6.95$15.06€10.28$11.43 delivery to United States Receive this item 5-10 working days from dispatch International delivery available materialmaterial (required)Gold plated (18ct)Rhodium platedYellow gold (9ct) (+ £350)White gold (9ct) (+ £350)Rose gold (9ct) (+ £350)material (required)Gold plated (18ct)Rhodium platedYellow gold (9ct) (+ $758.45)White gold (9ct) (+ $758.45)Rose gold (9ct) (+ $758.45)material (required)Gold plated (18ct)Rhodium platedYellow gold (9ct) (+ €517.96)White gold (9ct) (+ €517.96)Rose gold (9ct) (+ €517.96)material (required)Gold plated (18ct)Rhodium platedYellow gold (9ct) (+ $575.82)White gold (9ct) (+ $575.82)Rose gold (9ct) (+ $575.82) precious stoneprecious stone (required)Diamond (+ £100)precious stone (required)Diamond (+ $216.70)precious stone (required)Diamond (+ €147.99)precious stone (required)Diamond (+ $164.52) quantity by EVJ Location: London, GB read seller's profile see seller's complete range more items from this seller Gold Plated 'Never Give Up' Bracelet £200$433.40€295.98$329.04 Gold Plated 'Surprise Me' Necklace £250$541.75€369.97$411.30 Gold Plated 'Truly Blessed' Bracelet £200$433.40€295.98$329.04 Gold Plated 'Truly Blessed' Necklace £250$541.75€369.97$411.30 Gold Plated 'Live Your Dreams' Necklace £250$541.75€369.97$411.30 Gold Plated 'Птица Гордая' Bracelet £200$433.40€295.98$329.04 view all you may also like PRODUCT DETAILS DELIVERY RETURNS Made in Britain ask seller a question save for later Surprise Me Necklace spelled out in our signature EVJ hand-drawn script. Made in London. Made to Order. 5 - 7 days The gorgeous gold plated piece is delicate and crafted to perfection and comes with fine chain and clasp closure. Jewelry designer Elizaveta Yurlasova lets girls get exactly what they want, a man with a clue. For every girl who's wanted a boyfriend who knows how to surprise her, EVJ Jewelry has you in mind. The "Surprise me" necklace seems like the perfect gift for the woman who's often told her man the most dreaded words in gift giving, "Just surprise me". Last Order Date for Saint Valentine's Day: the 5th of February made from: 18ct Yellow Gold Plated Sterling Silver Fine chain. Suprise Me pendant. dimensions: Necklace Length 25cm. product code: 202262 This product is dispatched to you by EVJ. MAINLAND UK order now and receive this item on or before price Free Delivery Friday 16 October FREEFREEFREEFREE NON-MAINLAND UK estimated delivery price Highlands & Islands of Scotland 2-5 working daysfrom dispatch £1.75$3.79€2.58$2.87 Northern Ireland 2-5 working daysfrom dispatch £1.75$3.79€2.58$2.87 Other UK Islands 2-5 working daysfrom dispatch £3.95$8.55€5.84$6.49 INTERNATIONAL DELIVERY estimated delivery price Western Europe 2-5 working daysfrom dispatch £5.00$10.83€7.39$8.22 Rest of Europe 5-10 working daysfrom dispatch £5.00$10.83€7.39$8.22 USA & Canada 5-10 working daysfrom dispatch £6.95$15.06€10.28$11.43 Asia & Australasia 5-10 working daysfrom dispatch £7.25$15.71€10.72$11.92 Rest of the world 5-14 working daysfrom dispatch £7.25$15.71€10.72$11.92 You have 28 days, from receipt of cancellable goods, to notify the seller if you wish to cancel your order or exchange an item. Please note: goods that are personalised, bespoke or made-to-order to your specific requirements, perishable products and personal items sold with a hygiene seal (cosmetics, underwear) in instances where the seal is broken are non-refundable, unless faulty. Read more about our returns policy.Should you choose to return or exchange your order you will need to deliver the item(s) to the UK, where this seller is based. EVJ's return policy


text: Stockbyte Impatient Officer Destroys Car Window During Nasty Traffic Stop By GuySpeed Editors March 30, 2015 | GuySpeed If you thought everyone in Canada is as sweet as sticky maple syrup, here's your wake-up call. Read More|Comment Category: Accidents, Crime, Daily Distraction, Videos, Weird News Tags: Officer, Window Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


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The Journal Sentinel business department follows developments in the local retail industry
Metro Market coming to Brookfield
Roundy's Supermarkets Inc. wants to turn its Pick 'n Save store in the Brownstones shopping center on W. Blue Mound Road in Brookfield into a Metro Market, according to a plan submitted to the city.
The proposal will be on the agenda for the April 6 Plan Commission meeting, said Dan Ertl, director of development for Brookfield. As part of the plan, the vacant former Hollywood Video store would be torn down to allow more parking.
Roundy's organizational communications director Lynn Guyer confirmed the Metro Market plan and said the company hopes to expand the store as part of the makeover to the high-end grocery concept. The store, at 17630 W. Blue Mound Road, is the oldest one in the chain. It opened in 1975 as a warehouse food store.
Roundy's operates only one Metro Market, at 1123 N. Van Buren St., in downtown Milwaukee. The store, which opened in August 2004, includes a coffee bar with a fireplace and plasma screen TV, and Wi-Fi Internet access.
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These movies are awesome.
Mr. Boomba
Mr. Boomba tries to impress the girl of his dreams, but always seems to find a way to humiliate himself!
Eskimo Bob
Eskimo Bob, he eats lots of fish!
How is this a disgrace?
it aint, its pretty well made.Kudos,
haha! that was great!! MORE MORE MORE!!! lol
a parody of a parody of a parody of a game that wasn't even that good/popular to begin with.... and before people start biting my head off for that.... OLD SKOOL ROCKS! special thanks to evilzug for getting me hooked on this song.... i still like the pixelated white chick with bad moves over cutman though. :p
I love that song!
I really like this song I hope you make more of this song
That was great. Gotta love that song. I hope more like that appear.


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Beautiful setting and gardens. Two highly-rated restaurants on premises. Heated pool and lovely pool setting. Friendly staff; immediate response to requests. Spacious room; useful mini-kitchen with prep counter, sink, refrigerator, and utensils. Comfortable bed. Ample room lighting. Convenient work desk and sitting table in room.
Mix of contemporary and baroque furnishings is odd. No microwave in room. No hot tub/jacuzzi at pool. No fresh (refrigerated) half-and-half available for the coffee in the lobby.
Clean, quiet, basic motel room.
No option for three adults to comfortably stay in the same room.
Coffee was not complimentary bu the doughnut holes were, weird.
convenient to destination, snacks, pool area, business center
noisy sports teams clogging lobby
The room was big and it looks like they take care of their rooms
Everything was perfect for one night
Room had modern fixtures and nicely decorated. Free breakfast. Free Wi-Fi. Free Parking. Hotel staff very friendly.
Curtains in the room were not designed to fully close, which allowed for outside light to come in at all times, as well as less than complete privacy.
Clean, nice furnishings, good people
A bit inconvenient
Spacious studio, clean, kitchen in room, complimentary breakfast!, great for families, will look into Residence Inns for future travels
Shower curtain smelled slightly mildewy, lotion was half used already when we got there
It's very clean! I would definitely recommend others to stay here.
What I didn't like was that the Restroom Door is See-through.
The staff was extremely nice and professional. I felt so welcomed. Clean hotel!!! I would definitely come back.
Clean, fast WiFi, nice art print on the wall. Adequate parking, and of course a low price in a costly area.
Lumpy bed, loud and not very effective AC, laughable breakfast, bit of a sketchy vibe if you're the sort to spook easily. Slow front desk everything.
The hotel has an excellent location on El Camino Real in Mountain View. It is set back enough from the street so that traffic noise is not objectionable. The rooms are basic but clean, with good internet. The value for price is excellent compared with other motels located along El Camino Real in the same area. We have stayed there several times before, and intend to do so again.
You could hear every footstep from person in the room above.
I just liked everything about it nothing really negative.
If I had to be picky the fact there wasn't many movies on the tv but everything is HD so how can I really complain?
Great location, clean room, friendly service, above-average breakfast, fast WiFi, nice layout.
Can't think of any complaints.
Friendly staff, great breakfast buffet with custom omelettes, comfortable bed, heater and frig not too loud, convenient right off hwy 85. Lots of places to eat close by. Near to Stanford. Much better value than Domain Hotel nearby.
The morning breakfast is quite good with very good selection of items that change on a daily basis. Great location within walking distance of many good restaurants.
Unable to check our via the television or phone. Have to check out at the front desk. It would be nice to be able to check out via either of those options.
The staff
location, staff
Dirty rooms, spider infestation.
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Lansdowne Hotel Ballsbridge
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Free High-Speed Internet
- Room service
- Restaurant
- Business Services
- Wheelchair accessibility
- Free parking
TripAdvisor Reviews Lansdowne Hotel Ballsbridge Dublin
Travel Blogs from Dublin
Glendalough - The Glen of the Two Lakes
... you about Dublin city to entice you here, unless you really like pubs. For me, it is an old city, with some really unattractive buildings, but some really pretty ones too. There seems to be a story behind all of the old buildings which is great and it seems that the animosity between the north & south of Dublin is quite evident. The river Liffey separates them, but there is a lot of rivalry. By the ...
I am water, I am a tree
... I would not survive. It is the oceans and all other bodies of water that maintain my sanity, that bring me back to this moment, that wrap me in their warm waves as I dive in to be enveloped in their silky embrace. Water is my protector, my magic cloak, my saviour and my guide. I bow to you.
And yet I still wish to stand tall. I want to breathe, I want to provide, I want to shelter. For I am water, and I am a tree.
... we were ready to roar
Some Irish dancing we tried to learn
Carmels face was very stern
And full of concern
Kar's limerick
Bob thinks the bollards in Derry
Are just a bit to scary
Whilst walking down the street
He came to grief
It left him battered & bruised
He was not amused
And now he talks like a ...
Lecker Essen
... and the museum part of it, and I stayed a bit longer than they did.
But before they left I arranged for me to cook pasta for all of us. It was my last night in a hostel and I had far more pasta than I needed. Why not cook some Essen for the Germans? Well two of.
At 6 something we ate dinner and talked and then I went ...
Two days in Dublin
... at the same time.
We have been having some nice meals and a few Guinness beers along the way. Very enjoyable to say the least.
The people here are extremely helpful and friendly. The Irish have mastered the tourist business very well. At the airport I told an attendant I was lost and asked for directions. He asked if I was supposed to be in Ireland and when I said yes he said I wasn't ...


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Ovation Lauren Pull On Jods - Kids, EuroSeat
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Item Code : ER90958
Comfy Kid's Jods!
The Ovation® Kids Lauren Pull On Euroseat Jodhpurs are stretchy and comfy jodhpurs that are made of light weight cotton/spandex jersey knit fabric.
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The Ovation®Kids Lauren Pull On Euroseat Jodhpurs offer:
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text: http://askubuntu.com – Ok, so every weekend for a few weekends in a row I've tried (each time unsuccessfully) to install Ubuntu as a dual-boot on my MacBook pro 15-inch (2011). Previously, I'd been trying to do it from a USB stick, but then I read that that sometimes creates problems, so now I'm doing it with a DVD-ROM. I've been using the .iso downloaded from this page. (12.04.1 AMD64 Desktop CD) I have performed a MD5SUM check on the .iso, which results in 14984b15a391c7de1f07f4885bef2d5c (which is the correct hash) I have burned it at the lowest possible speed on my disk drive with 2 different disks. I made a (HowTos)


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ACE Fit Share
Browse through a variety of lower and upper chest exercises designed by today's leaders in the health fitness industry. We have strength training exercises for beginner, intermediate and advanced users. These exercises will help you increase the strength and definition of your chest area. If you want more information on chest exercises or strength training of any kind, be sure to check out our Fit Facts section and Ask the Expert column. These chest exercises are also featured in full workout routines in our Workouts section.
Fit Life Articles
You'll find three types of people in the fitness world. Learn how to be the one who achieves results.
Many people spend a large amount of time sitting down—at a desk, in the car and while watching television. As a result, they may experience tight hips, limited range of motion or chronic back pain. Performing these three effective hip openers can go a long way toward...
Hitting the road this summer? Whether you're on a weekend getaway or an extended business trip, it can be tough to stick with your health and fitness goals while traveling. Technology expert Ted Vickey rounds up the latest apps to help you stay fit and focused when travel...


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Zumba with AhvaRead MoreAhva Halma began her love affair with dance early in life, shortly after trading in her footie pajamas for a tutu and ballet slippers. As a young lady, she made her first foray into the world of Latin movement by studying flamenco, a journey she continues to this day in her Zumba workshops. Ahva's commitment to sharing the fun and fitness-building benefits of dance led her to earn seven types of Zumba teaching certifications. As a registered nurse, she's equally enthusiastic about women's health. This is why Ahva additionally teaches Pilates and yoga courses with an emphasis on pre- and postnatal techniques.1200 E 57th St. at S Cliff Ave.Sioux Falls, SD US
Sioux Falls Martial Arts AcademyRead MoreBoost your energy at Sioux Falls Martial Arts Academy in Sioux Falls. Are you tough enough? To complete a cross fit challenge? You'll feel great when you finish it. Get to the next level of fitness by creating a fitness regime specialized to your needs with one of the gym's personal trainers. Sweat and fight your way to your fitness goals with a high-intensity MMA class. Burn a large number of calories and tone your abs, glutes, and legs with a kickboxing class from Sioux Falls Martial Arts Academy. Parking is plentiful, so patrons can feel free to bring their vehicles.4515 E 26th StSioux Falls, SD US
X fitnessRead MoreX Fitness collects the efforts and skill of four personal trainers into one intimate gym experience, which members have access to 24 hours a day. The staff teaches eight different fitness classes, including gym standards such as boot camp and more unique courses such as B.L.T., which stands for Buns, Legs, and Thighs. To complement the workout regimen, they also offer 24-hour tanning services and personal training during normal business hours.112 W 39th St.Sioux Falls, SD US
Sioux Empire FitnessRead MoreMake yourself a member at Sioux Empire Fitness in Sioux Falls, and enjoy working out at a spot you'll love. Learn how to defend yourself with kickboxing. Whether you prefer street or lot parking, Sioux Empire Fitness is located near both options.4600 S Tennis LnSioux Falls, SD US
She's Shapin UpRead MoreCheck out She's Shapin Up in Sioux Falls and put an end to "putting it off". Learn how to properly defend yourself with one of these kickboxing classes. Parking is plentiful, so visitors can feel free to bring their vehicles.2111 W 49th StSioux Falls, SD US
The Dharma RoomRead MoreThough The Dharma Room is a yoga and wellness studio, it often reverberates with the telltale sounds of a comedy club or a dentist's office with leaky nitrous-oxide containers. Owners Jill Johnson and Sean Gallup put a playful spin on the ancient practice during Laughter yoga classes, a style that mingles breathing exercises with spontaneous giggles. The sessions reflect an overall commitment to finding joy through the studio's yoga and holistic health services, which include massage, reiki, and meditation in addition to the class curriculum. The Dharma Room's staff endorses acceptance of all guests regardless of experience level or age. Their workshops often stretch beyond the scope of traditional yoga, tapping into happiness reserves with tai chi and crystal-singing-bowl meditation.1604 S. Western Ave.Sioux Falls, SD US