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ISIS and Muslim Brotherhood Killing Christians
in War
PLEASE SHARE: Episode #319: Voices of Global Freedom Radio is interveiwing Dr. Ashraf Ramelah besieged Middle East Coptic Christian Leader.
"Dr. Ramelah briefs Presidents, parliaments, on Coptic Christian oppression in Egypt, Iraq, and Syria," says Roy ('Backpack') Baron host of Voices of Global Freedom Radio ."Coptic Christians are targeted for beheading and turture by ISIS, Muslim Brotherhood, and Al-Qaeda Jihadist Terrorists throughout the Middle East".
Dr. Ramelah, founder and President of the Coptic Christian organization "Voice of the Copts", talks live on the mass genocide of Coptics http://www.voiceofthecopts.org
Don't miss this lively, high energy, entertaining, informative show covering current threats to our liberty, actionable tactics and strategies to survive and thrive in these dangerous troubled times.
Voices of Global Freedom Archives
Muslim Brotherhood-Sharia Law-USA
in Politics
We will discuss the history Sharia Law, what's in it, what Muslim Brotherhood and CAIR are bringing to Americas shores with Billions of Dollars of funding. President Obama is in favor of this. We will present proof of all that is said. This is for your children's future
PIJN News: Egypt Sentences Muslim Brotherhood Ex-President to Death
in Christianity
Dr. Chaps reports on these important issues:
* Egypt has just sentenced their Muslim Brotherhood Ex-President to death.
* President Obama continues to pressure Israel to divide Jerusalem.
* Congress has now subpoenaed Hillary Clinton's top aide to testify about Benghazi.
The Muslim Brotherhood
(Personally, I prefer the term "moslem" as they find that insulting, but for sake of edification...)
..and speaking of edification, I referred to PRESIDENT Hosni Mubarak as "Prime Minister." My deepesh, sheepish, apologies...
"God is our objective. The Prophet is our leader. The Qur'an is our law. Jihad is our way. Dying in the way of God is our hope." ~ The Egyptian Muslim Brotherhood motto...
Welcome to Red Fox Blogger with your Host, Kel Fritzi, heard here Mondays and Thursdays (and the odd impromptu show) @ 3PM ET
I will return to Prime Time and phone calls in September.
Also, follow RFB @ redfoxblogger.blogspot.com
The Betrayal Papers- Pt I - Under Obama, US Captured by Muslim Brotherhood
The Betrayal Papers will trace the influence of the Muslim Brotherhood in the Obama administration's foreign and domestic policies.
The six-part series will present Muslim Brotherhood USAa picture of a conspiracy that is manipulating the American government to the benefit of a totalitarian, genocidal movement that seeks to establish a global Islamic State.
The Betrayal Papers -- Obama's connection to the Muslim Brotherhood.
in Politics
The Betrayal Papers reveal Obama's submission to the Muslim Brotherhood. Joining guest host, Andrea Shea King, will be a panel of researchers who have come together to reveal the Muslim Brotherhood's ties to Obama, its influence on his administration and the problems they've caused in the world.
We'll explore Obama's fascistic approach to the American economy, and Muslim Brotherhood's fingerprints along with those of George Soros on various domestic policies and scandals.
In-depth articles on Common Core, the transformation of DHS, 'comprehensive' immigration reform, demonization of military vets as domestic terrorists ar part of their research and has shown that these are all related to the Muslim Brotherhood within our government.
After months of research, the Coalition of Concerned Citizens has published three of five formal articles with the intent of proving beyond a reasonable doubt that Obama is a Muslim Brother and is directing US policy to their ends. On the show: William Palumbo, founder of the Coalition of Concerned Citizens, a group of retired military, researchers, analysts, and journalists who are focused on exposing the Muslim Brotherhood in the US. Bethany Blankley, journalist and conservative columnist for the Washington Times. She is a political commentator for Fox News and has appeared on several networks.
Wallace Bruschweiler, active with intelligence and counter-terrorism on four continents for three decades.
The Awakening - Unmasking the Muslim Brotherhood w/Clare Lopez
For G_d, For Country, For Freedom.
Tonight a very special night at The Awakening as we welcome Clare Lopez, Vice President for Research and Analysis at the Center for Security Policy. Ms. Lopez and I will drill down deep into the workings and goals and tactics of the Muslim Brotherhood.
Ms. Lopez manages the Center's counterjihad and shariah programs, bringing with her also an expertise on Iran, Hizballah, and southern border issues. From 2010-2014, she was a Sr. Fellow with the Center. Lopez began her professional career as a CIA operations officer and later applied her national security expertise as a consultant, intelligence analyst, and researcher in various contract positions within the defense sector. She has been an instructor for military intelligence and Special Forces students and lectures widely on Iran, Islam, and the Muslim Brotherhood around the country. Earlier an advisor to EMP Act America, in February 2012 Ms. Lopez was named a member of the Congressional Task Force on National and Homeland Security, which focuses on the Electro-Magnetic Pulse (EMP) threat to the nation and is a member of the Center's Secure the Grid Coalition.
Lopez is the co-author of two published books on Iran, author of an acclaimed paper for the Center, The Rise of the Iran Lobby, and co-author/editor of the Center's Team B II study, "Shariah: The Threat to America". Ms. Lopez received a B.A. in Communications and French from Notre Dame College of Ohio and an M.A. in International Relations from the Maxwell School of Syracuse University. She completed Marine Corps Officer Candidate School (OCS) in Quantico, Virginia before declining a commission to join the CIA.
Wm. Palumbo on Muslim Brotherhood deadly USA threats
in News
Episode #292: Exclusive! Voices of Global Freedom Radio Hosts Yoda and Backpack will interview President William Palumbo, President of the Coalition American Pariots daring to expose the deadly dreadfull threat to America - The Muslim Brotherhood.
The world's oldest Jihad terrorist organization has infiltrated the highest level of government and Fortune 500 Corporations. This terrorist group has the proactive support of Obama, Senators, Congressmen, and Captains of industry.
Listen and learn about this deadly enemy within and how they are infiltrating your city, effecting your job, your money, and your kids schools.
Learn how to protect yourself and your family.
Call in speak your mind, ask questions, and make comments.
(646) 652-4667
When: 4:00 PM (Pacific) on Saturday, December 13th, 2014.
The show will be available in archives http://www.blogtalkradio.com/voicesofglobalfreedom and on http://www.magnifiedview.com
Muslim Brotherhood Official Calling In From Egypt
in News
Don't miss Wael El` Manzalawy, Muslim Brotherhood official who will be calling-in live to our sister radio program, Voices of Global Freedom, on Saturday, October 4, 2014 at 4 PM Pacific time from Egypt. Wael reveals explosive facts about Islamic Jihadist attrocities and ambition to conquer America and the world with a Caliphate. In Wael's previous appearances Wael predictions have materialized.
We encourage callers so call in at (646) 652-4667
Learn more about hosts Backpack and Yoda:
Hidden Agendas, Islam, and Muslim Brotherhood on Prophecy Headline News hour
in Religion
Hidden Agendas, Islam, and Muslim Brotherhood on Prophecy Headline News:
Today we feature Hidden Agendas, Islam, and Muslim Brotherhood on Prophecy Headline News. It is here in the end of days we find the world caught up in the chaos of war, bloodshed, and God. It is here we find people that kill Christians "thinking they are doing God a service"! They kill to bring in their worldwide Caliphate, they deny the Father, and deny the truth of the Son of God Jesus Christ. Those who deny the Father and the Son are Anti-Christ.
WIBR/WARN Ministry sites: www.warn-radio.com www.warn-usa.com www.wingswatchman.org www.dday4america.com
The Muslim Brotherhood
This segment of Peter Hebert's On The Money will take a look at the Muslim Brotherhood by examining it's own website, the organization's mission and goals, and the implications for the world.
ISIS, al-C.I.A.da and Muslim Brotherhood are not a true Muslim groups.
in Politics
Tonite (8.28.2014) we exposed:
The lie of Big Brother that ISIS, al-C.I.A.da, Muslim Brotherhood, Hamas, Islamic Jihad, Hezbollah, etc. are grass root Muslim terrorist groups. They were all founded by Big Brother to do his bidding.
Big Brother plans to use his attacks on ISIS to knock off President Bashar al Assad of Syria and hand that nation over to ISIS.
The goal of Big Brother is to have a showdown between its Muslim dupes against the West ending in the total destruction of the Muslim nations (World War III). The demise of the Muslim nations is predicted in Ezekiel 38-39. Once the Muslim nations have been destroyed it will clear the way for Big Brother to establish its Old World Nightmare with the Antichrist as his puppet dictator.
Visit - FreeWorldFilmWorks.com for DVDs exposing the CRIMES of Big Brother.
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65pmblair wrote on JoseOmatic's wall
"Callsign: Nugget. Yo! Starbuck and I are going to frak up some cylons. You in? :) Hi, Dudette!"
JoseOmatic Of course!3 years ago
65pmblair Hahaha yesss! we still on for BSG later? *may not reply because she is going on Walk with ZR in a few*3 years ago
JoseOmatic Yup! Have fun!3 years ago


text:
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Multiple Sites of the Cleavage of 21- and 25-Mer
Encephalytogenic Oligopeptides Corresponding to
Human Myelin Basic Protein (MBP) by Specific Anti-MBP
Antibodies from Patients with Systemic Lupus
Anna M. Timofeeva1, Pavel S. Dmitrenok2, Ludmila P. Konenkova3, Valentina N. Buneva1,4,
Georgy A. Nevinsky1,4*
1Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia, 2Pacific Institute of Bioorganic
Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, Russia, 3Institute of Clinical Immunology, Siberian Division of Russian Medical Academy of
Sciences, Novosibirsk, Russia, 4Novosibirsk State University, Novosibirsk, Russia
IgGs from patients with multiple sclerosis and systemic lupus erythematosus (SLE) purified on MBP-Sepharose in contrast to
canonical proteases hydrolyze effectively only myelin basic protein (MBP), but not many other tested proteins. Here we have
shown for the first time that anti-MBP SLE IgGs hydrolyze nonspecific tri- and tetrapeptides with an extreme low efficiency
and cannot effectively hydrolyze longer 20-mer nonspecific oligopeptides corresponding to antigenic determinants (AGDs)
of HIV-1 integrase. At the same time, anti-MBP SLE IgGs efficiently hydrolyze oligopeptides corresponding to AGDs of MBP.
All sites of IgG-mediated proteolysis of 21-and 25-mer encephalytogenic oligopeptides corresponding to two known AGDs
of MBP were found by a combination of reverse-phase chromatography, TLC, and MALDI spectrometry. Several clustered
major, moderate, and minor sites of cleavage were revealed in the case of 21- and 25-mer oligopeptides. The active sites of
anti-MBP abzymes are localised on their light chains, while heavy chains are responsible for the affinity of protein substrates.
Interactions of intact globular proteins with both light and heavy chains of abzymes provide high affinity to MBP and
specificity of this protein hydrolysis. The affinity of anti-MBP abzymes for intact MBP is approximately 1000-fold higher than
for the oligopeptides. The data suggest that all oligopeptides interact mainly with the light chains of different monoclonal
abzymes of total pool of IgGs, which possesses a lower affinity for substrates, and therefore, depending on the oligopeptide
sequences, their hydrolysis may be less specific than globular protein and can occur in several sites.
Citation: Timofeeva AM, Dmitrenok PS, Konenkova LP, Buneva VN, Nevinsky GA (2013) Multiple Sites of the Cleavage of 21- and 25-Mer Encephalytogenic
Oligopeptides Corresponding to Human Myelin Basic Protein (MBP) by Specific Anti-MBP Antibodies from Patients with Systemic Lupus Erythematosus. PLoS
ONE 8(3): e51600. doi:10.1371/journ `l.pone.0051600
Editor: Vladimir N. Uversky, University of South Florida College of Medicine, United States of America
Received October 17, 2012; Accepted November 1, 2012; Published March 8, 2013
Copyright: ? 2013 Timofeeva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was made possible in part by grants from the Presidium of the Russian Academy of Sciences (Molecular and Cellular Biology Program, 6.7;
Fundamental Sciences to Medicine, 5.13), Russian Foundation for Basic Research (10-04-00281), and funds from the Siberian Division of the Russian Academy of
Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
* E-mail: firstname.lastname@example.com
It is known, that the occurrence of auto-Abs in increased
concentration is a distinctive feature of various autoimmune
diseases (ADs) (reviewed in [1–8]). It was shown that small
fractions of auto-Abs can possess different catalytic activities [1–8].
Catalytically active artificial antibodies (Abs) or abzymes (Abzs)
against transition chemical states of different reactions were
studied intensively (reviewed in [1]). Healthy humans and patients
with many diseases with insignificant autoimmune reactions
usually lack abzymes or develop Abzs with very low catalytic
activities, with these activities being often on a borderline of the
sensitivity of detection methods (reviewed in [2–8]). Natural
abzymes hydrolyzing DNA, RNA, polysaccharides, oligopeptides
(OPs), and proteins are described from the sera of patients with
several autoimmune (systemic lupus erythematosus, Hashimoto's
thyroiditis, polyarthritis, multiple sclerosis, asthma, rheumatoid
arthritis, etc.) and viral diseases with a pronounced immune system
disturbance (viral hepatitis, AIDS, and tick-borne encephalitis)
(reviewed in [2–10]). Abzymes may play a significant positive and/
or negative role in broadening Ab properties, forming specific
pathogenic patterns and clinical settings in different autoimmune
conditions [1–10].
Multiple sclerosis (MS) and systemic lupus erythematosus (SLE)
are well known ADs. MS is a chronic demyelinating disease of the
central nervous system. Its etiology remains unclear, and the most
widely accepted theory of MS pathogenesis assigns the main role
in the destruction of myelin to the inflammation related to
autoimmune reactions [11]. Several recent findings imply an
important role of B cells and auto-Abs against myelin autoantigens
PLOS ONE | www.plosone.org1 March 2013 | Volume 8 | Issue 3 | e51600
Page 2
including myelin basic protein (MBP) in the pathogenesis of MS
SLE is a systemic autoimmune polyetiologic diffuse disease
characterized by disorganization of conjunctive tissues with the
paramount damage to skin and visceral capillaries [14]. The
polyetiologic and polysyndromic character of SLE leads to highly
variable manifestations of this disease in terms of many
biochemical, immunological, and clinical indices. SLE is usually
considered to be related to patient's autoimmunization with DNA,
since the sera of such patients usually contain DNA and anti-DNA
Abs in high concentrations [15]. At the same time, in comparison
with healthy donors, an increased concentration of auto-Abs was
observed for various antigens (% of patients): DNA (60),
cardiolipin (48), thyroglobulin (42), microsomal fraction of
thyrocytes (48), and rheumatoid factor (23) [5].
It should be mentioned, that SLE and MS demonstrated some
similarity in the development of the same medical, biochemical
and immunological indexes. MS is a chronic disease of the central
nervous system leading to the manifestation of different nervous
and psychiatric disturbances. However, neuropsychiatric involve-
ment occurs in about 50% of SLE patients and carries a poor
prognosis (reviewed in [11]). SLE predominantly affects the central
nervous system, and within its cerebral complications it has a
particular propensity-perhaps more than any other systemic
inflammatory disease– to cause psychiatric disorders [11].
Peripheral nervous system involvement is much less common.
The distinctive production of diverse auto-Abs seems to be related
to defective clearance of apoptotic cells. Antibody-mediated neural
cell injury and rheological disturbances represent the two principal
suggested mechanisms of tissue injury [11]. Interplay between
these processes, underlying genetic factors, their modification by
hormones, complicated by a number of secondary factors, may
explain the wide spectrum of features encountered in this disease.
Some indicators of disease common to SLE and MS were
observed [11].
For diagnostics of MS, thirteen Poser's medical indices are often
used [16], but clinically definite MS diagnosis is usually based on
the tomographic detection of specific plaques in the brain, which
appear on late stages of not only this disease, but also in SLE
patients. Similarly to SLE, the high-affinity anti-DNA Abs has
been recently identified as a major component of the intrathecal
IgGs in MS patient's brains and cells of the cerebrospinal fluid
[17]. It was recently shown that titers of Abs against human myelin
basic protein in SLE patients 4.2-fold higher than in healthy
individuals, but 2.1-fold lower than in patients with MS [9]. In
addition, abzymes from the sera of patients with SLE and MS
possess by the same catalytic activities (see below).
It was shown that SLE IgGs and IgMs effectively hydrolyzed
DNA, RNA, and polysaccharides [18–22]. Similarly to SLE,
homogeneous IgGs from the sera and the cerebrospinal fluid of
MS patients were active in the hydrolysis of DNA, RNA, and
polysaccharides [23–25]. Whereas only 18 and 53% of MS
patients contained increased concentrations of Abs to native and
denatured DNA, respectively, as compared with healthy donors,
DNase abzymes were found in ,80–90% of MS patients [23–24].
Since DNase abzymes of MS patients [4] similarly to SLE patients
[26] are cytotoxic and induce apoptosis, they can play an
important role in SLE and MS pathogenesis.
It has been recently shown that MBP-hydrolyzing activity is an
intrinsic property of IgGs, IgMs, and IgAs from the sera of MS
patients [27–30] and the specific sites of the neural antigen cleaved
by abzymes have been established [31]. Recognition and
degradation of MBP peptides by serum auto-Abs was confirmed
as a novel biomarker for MS [31]. In MS, anti-MBP abzymes with
protease activity can attack MBP of the myelin-proteolipid sheath
of axons. The established MS drug Copaxone appears to be a
specific inhibitor of MBP-hydrolyzing activity of the abzymes [31].
Consequently, MBP-hydrolyzing abzymes may play an important
negative role in MS pathogenesis. At the same time, the similarity
in some immunological indexes between MS and SLE can speak
in favour of that anti-MBP Abs with proteolytic activity can occur
in SLE patients. Recently, it was shown that electrophoretically
and immunologically homogeneous IgGs (approximately 86% of
SLE patients) purified using several affinity resins including
Sepharose with immobilized MBP (MBP-Sepharose) specifically
hydrolyze only MBP but not many other tested proteins [9].
Several rigid criteria were applied to show that the MBP-
hydrolyzing activity is an intrinsic property of SLE IgGs but not
from healthy donors. It was shown, that the immune systems of
individual SLE similarly to MS patients can generate a variety of
anti-MBP abzymes with different proteolytic properties, which can
attack MBP of myelin-proteolipid shell of axons and play an
important role in pathogenesis not only MS but also SLE patients.
Anti-MBP abzymes from the sera of MS patients hydrolyze
MBP at several sites localized within four known immunodomi-
nant regions of MBP [31]. In addition, it was shown that anti-
MBP abzymes from the sera of SLE patients hydrolyze MBP at the
same four immunodominant sites of MBP [9]. Four peptides
corresponding to known immunodominant regions of MBP are
encephalytogenic and can play a negative role in the MS and SLE
pathogenesis [31].
Taking this into account, it was interesting to study the Ab-
dependent hydrolysis of MBP specific sequences in more detail. In
this paper, we have analyzed site-specific degradation of two
oligopeptides (21- and 25-mer) corresponding to two AGDs of
MBP using combination of reverse-phase
(RPhC), thin-layer chromatography (TLC), MALDI spectrometry,
affinity chromatography, and enzymic kinetics.
Abzyme characterization
In this work, electrophoretically and immunologically homoge-
neous polyclonal IgGs (pIgGs) were purified from the sera SLE
and MS patients as well as healthy donors by sequential
chromatography of the serum proteins on Protein G-Sepharose
under conditions that remove non-specifically bound proteins,
followed by gel filtration under the conditions that destroy
immune complexes as in [9]. Electrophoretical and immunological
homogeneity of the pIgGs was confirmed respectively by SDS-
PAGE with silver staining and by Western blotting similarly to
[9,27–30]. To analyze an ''average'' situation, we have prepared a
mixture of equal amounts of homogeneous pIgGs from the sera of
ten SLE (sle-IgGmix), ten MS (ms-IgGmix) patients, and ten healthy
donors (hd-IgGmix). Then sle-IgGmix, ms-IgGmix, and hd-IgGmix
preparations having affinity for hMBP were separated by affinity
chromatography on MBP-Sepharose as in [9]. The fractions of
IgGmixeluted from MBP-Sepharose with 3 M NaCl were used in
this study. To exclude possible artefacts due to traces of
contaminating proteases, these fractions were separated by SDS-
PAGE and their proteolytic activity was detected after the
extraction of proteins from the excised gel slices as in [9]; only
sle-IgGmix and ms-IgGmix preparations were active, when hd-
IgGmixwas catalytically inactive. The detection of MBP-hydro-
lyzing activity of these Abs similarly to [9] in the gel region
corresponding only to IgGs (150 kDa) together with the absence of
any other band of the activity or protein, provided a direct
evidence that all pIgG preparations used are not contaminated
Multiple Sites of Myelin Basic Protein Cleavage
PLOS ONE | www.plosone.org2March 2013 | Volume 8 | Issue 3 | e51600
Page 3
with canonical proteases. In addition, similarly to [9] it was shown
that, in contrast to canonical proteases, the SLE and MS IgGmix
purified on MBP-Sepharose specifically hydrolyzed only MBP but
not many other tested proteins.
Ab-dependent hydrolysis of oligopeptides
It was shown previously, that thyroglobulin-directed proteolytic
IgGs effectively hydrolyzed not only thyroglobulin but also Pro-
Phe-Arg-methylcoumarinamide (MCA) at the Arg-MCA bond
with a significantly lower affinity [32]. Polyclonal IgG preparations
from patients with rheumatoid arthritis also displayed Pro-Phe-
Arg-MCA-hydrolyzing activity [32]. Anti-integrase IgGs and IgMs
from HIV-infected patients hydrolyze not only globular HIV-1
integrase but also different specific and nonspecific tri- and
tetraoligopeptides [33,34]. Therefore, first we have analyzed the
efficiency of hydrolysis of nonspecific tri- and tetrapeptides using
sle-IgGmixand ms-IgGmixpurified by affinity chromatography on
MBP-Sepharose. It was shown that anti-MBP sle-IgGmixand ms-
IgGmix cannot hydrolyse short peptide Pro-Phe-Arg-MCA (sh-
OP1), but after 24–48 h of the incubation they cleavage Boc-Val-
Leu-Lys-MCA (sh-OP1) and Boc-Ile-Glu-Gly-Arg-MCA (sh-OX3)
with very low efficiency (Fig. 1A). The relative rate of the MCA
formation was approximately 4-5- (sh-OP2) and 20-25-fold (sh-
OX3) higher in the presence of ms-IgGmixthan that for sle-IgGmix.
Similar situation was observed for longer nonspecific 20-mer
oligopeptides in-OP1 and in-OP2 (Fig 1B) corresponding to viral
integrase, that, as revealed by a MALDI analysis, contain several
sites of IN cleavage in the case of anti-IN IgGs from HIV-infected
patients [33,34]. Nonspecific in-OP1 and in-OP2 oligopeptides
corresponding to HIV integrase were slightly hydrolyzed nonspe-
cifically after 24 of the incubation, but there was no detectable
difference in the fluorescence intensities of the spots after the
incubation of these OPs without (lanes 2) and with sle-IgGmix
(lanes 3) (Fig. 1B). Consequently, if sle-IgGmix can hydrolyze
nonspecific in-OP1 and in-OP2, this hydrolysis is a very negligible.
First, we have shown that all ten individual SLE IgG
preparations before purification on MBP-Sepharose produced,
according to TLC, the same products of specific (corresponding to
MBP) X-OP21 and X-OP25 oligopeptides cleavage, but every
preparation was characterized by a specific ratio of formation of
Figure 1. TLC analysis of the hydrolysis of different OPs. A, Boc-Val-Leu-Lys-MCA (shOP1), Pro-Phe-Arg-MCA (shOP2), and Boc-Ile-Glu-Gly-Arg-
MCA (shOP3) (5 mM) were incubated for 24 h without Abs (lanesC) and in the presence of 0.05 mg/ml MS IgGmixor SLE IgGmixpreparations (lanes
shown on the panel) demonstrating comparable relative activities in the hydrolysis of intact MBP. B, Nonspecific in-OP1 and in-OP2 were incubated
for 24 h without Abs (lanes 1) and in the presence of 0.05 mg/ml SLE IgGmix(lanes 2); lanes C correspond to in-OP1 and in-OP2 before incubation. C,
Specific X-OP21 (C) and X-OP25 oligopeptides were incubated for 7 h with hd-IgGmix(lane C) and in the presence of 0.02 mg/ml sle-IgGmix. These OPs
were used in different concentrations (mM): 0.05 (lanes 1), 0.1 (lanes 2), 0.2 (lanes 3), 0.3 (lanes 4), 0.4 (lanes 5), and 0.5 (lanes 6).
Multiple Sites of Myelin Basic Protein Cleavage
PLOS ONE | www.plosone.org3 March 2013 | Volume 8 | Issue 3 | e51600
Page 4
different cleavage products. In addition, using TLC it was shown
when sle-IgGmix and ms-IgGmix after purification on MBP-
Sepharose produce the same X-OP hydrolysis products, but at
remarkably different ratios. To analyze in more detail an
''average'' situation concerning hydrolysis of X-OP21 and X-
OP25 by SLE anti-MBP IgGs we have used sle-IgGmix; Figs 1C
and 1D demonstrate efficient hydrolysis of these specific
encephalytogenic OPs by sle-IgGmix.
Thus, anti-MBP IgGs from SLE patients can efficiently
hydrolyze not only globular molecules of MBP (not many other
tested proteins) [9], but also specific OPs corresponding to AGDs
of MBP. Interestingly, sle-IgGmix-dependent proteolysis of both
specific OP21 and OP25 leads to the formation of several products
corresponding to 5-10 fluorescent spots (Fig. 1).
The dependency of the initial rate on the X-OP21 and X-OP25
concentrations in the reaction catalyzed by sle-IgGmix was
consistent with Michaelis–Menten kinetics (for example, Fig. 2).
The Km and apparent kcat values for X-OP21 (2.860.3 mM;
1.160.1min21) and X-OP25 (1.660.2 mM; 1.460.2 min21) were
MALDI spectrometry analysis of specific peptides
Fig. 1 demonstrates that hydrolysis of specific X-OP21 and X-
OP25 by anti-MBP sle-IgGmix produces several fluorescent
oligopeptides, the relative amounts of which increase with the
increase in the concentration of these OPs. TLC alone cannot
unambiguously determine the sequences of these products, since
their TLC mobility depends on many factors including the amino
acid content, relative hydrophobicity, the nature of the terminal
amino acids, etc. To identify major sites of IgG-mediated
proteolysis of these OPs, we analyzed products of peptide cleavage
by a combination of RPhC, TLC, and MALDI massspectrometry.
First, we have analyzed the products of nearly complete X-
OP21 hydrolysis after 7 h of incubation (Fig. 3). Seven major and
several very small peaks corresponding to fluorescent products of
X-OP21 hydrolysis were revealed by RPhC (Fig. 3A). The
products of all peaks were analyzed by TLC (Fig. 3B) and by
massspectrometry (Figs 3C and 3D). One can see that only the 4th
and 7thRPhC peaks according to TLC contain a single
predominant product of the hydrolysis. According to TLC and
massspectrometry major peak 2 contains seven products of the
cleavage (n=6, 7, 8, 9, 10, 12, and 13) and initial non-cleaved X-
OP21 having comparable affinity to RPhC-resin (Fig. 3A), but
different mobility at TLC (Fig. 3B). Badly separated 4thand 5th
peaks (Fig. 3A) contained mainly 4- and 5-mers (Fig. 3D), while 7th
peak 2- and 3-mer X-OPs.
Fig. 4A demonstrates the data of RPhC of the cleavage products
corresponding to X-OP21 after its complete hydrolysis (12 h). In
this case there was observed only four major and many very small
peaks. According to TLC (Fig. 4B, lane 2) and massspectrometry
(Fig. 4C), peak 2 contained mainly three products: 6-, 7-, and 8-
mer X-OPs. Peak 3 corresponded to 4-mer (Fig. 4D), while 4th
peak to 2-mer X-OP (Fig. 4E). Thus, long products of the
hydrolysis finally can be hydrolyzed by SLE-Abs to short X-dimer
and X-tetramer. In addition, after a long incubation small amount
of a free X-fluorescent compound and X-monomer were revealed
(peak 1, Figs 4A and 4B) using TLC and massspectrometry. It
should be mentioned that massspectrometry analysis of products of
X-OP21 hydrolysis after 3 h of incubation has shown that three
OPs with low TLC mobility (Fig. 1C) correspond to 10-, 12-, and
13-mer X-OPs.
Nine brightly expressed and several additional very small peaks
corresponding to fluorescent products of X-OP25 medium
hydrolysis were revealed by RPhC (Fig. 5A). After RPhC the
same products of the hydrolysis were identified by TLC and by
massspectrometry in several peaks (Fig. 5). It means that some
individual products of X-OP25 hydrolysis can be eluted from the
sorbent by different concentrations of acetonitrile. The reaction
mixture contains Tris-HCl and trifluoroacetic acid, which
components can interact with positively and negatively charged
amino acid residues of products of the X-OP25 hydrolysis. In
addition, one cannot exclude that some of OPs can form
interpeptide complexes. An existence of multiple forms of the X-
OP products can lead to the elution of these forms of the same X-
OPs from the sorbent by different concentration of acetonitrile.
Using the combination of RPhC, TLC, and MALDI-TOF
analysis of the products of the X-OP25 hydrolysis were identified.
For example, the highest peak 4 according to TLC (Fig 5B, lane 4)
and massspectrometry (Fig. 5C) contained several X-OPs: 5-
demonstrates the spectrum of initial X-OP25 and the signals of
Figure 2. Determination of the Kmand Vmaxvalues for OP21 (A)
and OP25 (B) in the reaction catalyzed by sle-IgGmix(0.1 mM)
using a Lineweaver–Burk plot. The reactions were performed as
described in Materials and Methods. The average error in the initial rate
determination from two experiments for each substrate concentration
did not exceed 7–10%.
Multiple Sites of Myelin Basic Protein Cleavage
PLOS ONE | www.plosone.org4March 2013 | Volume 8 | Issue 3 | e51600
Page 5
Figure 3. Profile of RPhC of SLE IgGmix-dependent products of X-OP21 relatively deep hydrolysis (A) and analysis of X-OP products
of the hydrolysis corresponding to different peaks after RPhC by TLC (B) or massspectrometry (C–E): (–), relative fluorescence (A).
Numbers of lines in panel B coincide with numbers of peaks on panel A; lanes C1, C2, and C3 correspond to the products of reaction mixture before
their separation by RPhC, X-OP25 incubated in the absence of IgGs, and a free fluorescent compound X, respectively. The arrows (and numerals on
Multiple Sites of Myelin Basic Protein Cleavage
PLOS ONE | www.plosone.org5 March 2013 | Volume 8 | Issue 3 | e51600


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Investors scouring details of the proposed Covidien acquisition might have noticed that Medtronic plans to indemnify certain officers and directors against the cost of an excise tax related to the deal.
The excise tax stems from a 2004 law that was meant to provide a deterrent to "inversion" deals in which a company moves headquarters overseas, as Medtronic is doing.
Medtronic officials stressed that the tax is distinct from the capital gains tax that all shareholders will face as part of the Covidien transaction.
"These gross-up payments will not cover any capital gains tax imposed on any exchange of shares of Medtronic stock held by Medtronic's directors or executive officers," the company said in a regulatory filing.
Those directors or officers "will be responsible for such capital gains tax just like all other Medtronic shareholders."
In the Covidien acquisition, the excise tax would only apply to about 10 top officials at Medtronic, according to the company.
Gross-ups to cover the excise tax let executives "focus on the welfare of the company, not their personal finances," Medtronic said in a statement. "They should not be discouraged from taking action that they believe is in the best interest of Medtronic and its shareholders."
But the idea is controversial.
"These people are fiduciaries, who are legally required to put shareholders' interests ahead of their own," wrote Allan Sloan, a senior editor at Fortune, in a magazine article published July 7.
Sloan joined a panel of experts testifying before a U.S. Senate committee July 22 about whether Congress should act to prevent inversion deals.
"The inversion virus now seems to be multiplying every few days," Sen. Ron Wyden, a Democrat from Oregon, said in prepared remarks.
"Medtronic, Mylan, Mallinckrodt and many more deals have either occurred recently or are currently in the works."
Sen. Orrin Hatch, a Republican from Utah, added: "The ultimate answer to this problem -- and the only way to completely address the issue of inversions -- is to reform our tax code."
Medtronic's acquisition of Covidien is set to close late this year or in early 2015.
Christopher Snowbeck can be reached at 651-228-5479. Follow him at www.twitter.com/chrissnowbeck.


text: I've created this website to help sell my house in Les Allues in the heart of the Three Vallees Ski Area, and to share my experiences of living for over twenty years in one of the worlds top ski resorts.I first came to the Three Vallees in 1994 and have had some fantastic times but all good things come to an end and it's time to think of hanging up my ski things and retiring.Perfect for both winter and summer with easy access to the Olympic Telecabin to Meribel Centre or the Meribus which runs via Meribel Village both within 500m of the Chalet. The nearest large town is Moutiers (17 minutes by car) where there are 3 modern supermarkets as well as the traditional markets each Tuesday and Friday mornings.The Eurostar runs direct from London (St Pancras) and Ashford twice a week to Moutiers during the winter season. There are 4 international airports within easy reach;Chambery 1 hr 10 minutesGeneva 2 hrsLyon St Exupery 2 hrsGrenoble St Geoirs 2 hrs


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Best of Entertainment
This picture taken on May 8, 2013 shows a sculpture made of 13... Photo d'actualitéArts Culture et Spectacles,Chine,Créativité,Décalé,Fabriquer,Horizontal,Province de Wuhan,Province du Hubei,Quartier résidentiel,Retraite,Sculpture,Ville,VoiturePhotographer STRCollection: AFP 2013 AFPThis picture taken on May 8, 2013 shows a sculpture made of 13 retired cars in 'Jiangcheng No.1' Creative Culture Park in Wuhan, central China's Hubei province. The sculpture, 'Rebirth 2013', is placed inside the park that used to be a factory back in last century. The first walking tractor of China was produced here in the factory in 1957. CHINA OUT AFP PHOTO (Photo credit should read STR/AFP/Getty Images)


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the reach adjust jammed up after a bit of a crash. i think the lever was pulled forward as it hit the ground, brake still works but can only adjust it with an allen key but if i move it from the position its in the lever becomes stiff to pull and locks up.
does anyone have a fix for this, also tried to take off the lever but i haven't a clue how to get it off so i can have a closer look.
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If the flash sensed the distance of that which you were focused on, knew what modifiers were attached, what kind of surfaces your were bouncing off of, how big the room is, etc., and altered the flash power to match that, your exposures for the subject at the plane of focus would be near perfect every time.
...but this is not how TTL flash metering works. This would be an impossible system to design for practical use. By the time you input all the variables, your shot would be gone.
You will get better flash exposures learning to adjust your flash power and/or f stop with distance than you will ever be able to get with any TTL flash meter....not to even mention incident flash meters....the best method for accurate flash exposures. They can be used in more hand held speedlight-lit situations than one would imagine (though not all of them).
E-TTL is a way to get some sort of workable exposure an average amount of the time for average people who who shoot average-toned compositions and cannot and/or will not do the above. It is not the way to get the ideal exposure.
Does this mean it has no use? Of course not! It just means that, like with any automated function, you have to be so aware of what it is doing, and always fudging it this way and that, that you are basically taking manual control of it anyhow! In this application, it can be a slight time saver, though you still need a manual thought process to get the best out of it.
It can also get you that quick, sloppy, ugly, lazy shot in a printable, though not ideal, way. IMO, this is its greatest use! Sometimes, having the freedom to not worry about being technically perfect can free you to snap pix at exactly the right time. An less-than-ideal exposure on a greatly-timed and positioned shot is better than a perfect exposure on a shot in which you missed your desired composition and timing.


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I am going through the source code for the above project and I don't understand the following lines of code can anyone help explain it to me please? I am trying to get the code to work with color images as it currently only works with greyscale images. I have the main methods working however the filters only get applied to the top quarter of the returned images.
//In the heeder file.
inline uint8_t* operator[](const int rowIndex) {
return m_yptrs[rowIndex];
//in the .mm file
void Image::initYptrs() {
m_yptrs=(uint8_t **) malloc(sizeof(uint8_t *)*m_height);
for(int i=0; i<m_height; i++) {
From my understanding it looks like it is creating a a reference to the pixels in the images however i don't understand this line of code.
Thanks in advance.


text:
Like most people, Hedrick Ellis grew up listening to his parents and grand parents tell family stories. As a teenager, he often tuned them out, but this year, eager to keep those memories alive, he hired a personal historian to interview his father and mother.
"You hear these stories over the years, but nobody ever really gets around to writing them down," says Mr. Ellis, of Arlington, Mass. "This seemed like an easy and practical way of capturing them."
In this age of the memoir, not all fascinating lives belong to notable individuals. Across the country, people like Mr. Ellis are part of a growing cottage industry of amateurs and professionals eager to preserve the experiences of older generations. Armed with notebooks, tape recorders and video cameras, they are coaxing a lifetime of memories from beloved relatives.
"We're seeing an increase both in the number of people who want to do personal historian work and an increase in the number of elders who want to be sure their stories are handed down," says Paula Stahel, president of the Association of Personal Historians.
She attributes much of the interest to changing family structures. In the past, she says, "We'd see our aunts, uncles, grandparents and cousins regularly. … Today's retirees pick up and move someplace else, or their children take jobs in distant cities, so we don't have the experience of living the stories together."
Some families hire a professional who is skilled in asking questions and shaping responses into a cohesive narrative. Others take a do-it-yourself approach.
"It's always a baby boomer who has children and aging parents," says David O'Neil, a personal historian in Newton, Mass. "They look at their parents and their children and wonder, 'What are my children going to remember about my own parents, and how do I capture and preserve their life stories?' As the World War II generation is passing away, there are a lot of efforts to record their stories."
Many people don't think they have stories to tell. One reluctant husband told Mr. O'Neil, "We'll be done in 10 minutes." An hour and a half later, he was still talking. Some women say, "All I've done is raise children."
Mr. O'Neil challenges that modesty, calling these storytellers "ordinary people with extraordinary lives." In addition to audio and video formats, people preserve memories in books, ranging from simple bindings to customized leather editions.
Others use the Internet, answering questions online to create an "instant autobiographical book." Prices for professional services vary widely. Four hours of audio interviews can start at $500, Mr. O'Neil says. Transcription into a book with photos can cost more than $5,000.
Some families use a team approach. Last year, Dallas resident Scott Tims and his family interviewed his 94-year-old maternal grandmother on video.
Before they began, he created an outline of topics and questions to ask. He was careful to avoid sensitive subjects. "The challenge was keeping the interview on track, not allowing my family members to get us off on a tangent or tell her stories for her," he says. "Everyone learned interesting things about Nanny."
Dennis Stack, founder of Project StoryKeeper (www.sto rykeeper.org), makes a case for one-on-one interviews. He hopes to train 100,000 volunteers to capture the stories of older generations.
Using outsiders as interviewers offers a new audience, whereas family members are likely to say, "I don't need to hear that story; I already know it."
Donna Gold of Stockton Springs, Maine, discovered family stories she had never heard when she traveled to California to record her great-aunt's memories of pogroms in Ukraine and the family's journey to the United States.


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What is a monopoly?
The concept of a monopoly is largely misunderstood and the mere mention of the term evokes lots of emotions that make clear judgment almost impossible. The standard economic and social case for or against monopolistic businesses is no longer straightforward.
According to Mankiw (2009) a monopoly is defined as a market structure characterized by a single seller of a unique product with no close substitutes[1]. When a business dominates a market, it becomes a monopoly by virtue of its power. A company (or a group of affiliated companies) is considered to have a dominant position in a particular market if it exerts a decisive influence over the general conditions of trade in that market or can restrict access to that market for other businesses. Markets keep changing with the times and so are the conditions in which businesses must operate regardless of whether they have any noticeable market power.[2]
Monopolies have contributed significantly in transforming the US economy to be the leading economy worldwide. This is largely due to the benefits arising from legal monopolies created by the Patent and copyrights law. Monopolies are in effect powerful tools of spurring economic growth in the US.
How do monopolies arise?
Two major conditions contribute to formation of a monopolistic trade environment. A product which has no close substitutes faces no competition thus its producer becomes a monopolist. Exclusive ownership of a key resource may lead to creation of a monopoly. A classical case is exemplified by the control of the computer hardware, market by International Business Machines (IBM) for nearly forty years. Due to its market dominance over the hardware, institutions that intended to initiate a project had to do so with IBM. (Rise in Monopolies, n.d.) Monopolies also develop where there are barriers to market entry. These barriers are obstacles that make...


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Medium-deep ruby. Riper aromas of black cherry, licorice, minerals, spicy oak and menthol. Silkier and denser than the '98, but still on the hard side, with strong acidity and slightly green-edged blackberry and licorice flavors. Finishes with rather chunky, oak-enhanced tannins.Author: Stephen Tanzer
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Marco linear park trumps Gordon River project as Collier's top priority for federal money
NAPLES - The Gordon River Greenway Park is no longer Collier County's top priority on the a list of enhancement projects that could receive $800,000 in federal funding over the next two years.
Instead, the Colllier County Metropolitan Planning Organization on Friday moved the Marco Island Linear park trail project to the top of its list.
MPO members said their reason for moving the Marco project from fourth on the list to first was public safety.
The linear park trail from San Marco Road to Andalusia Terrace would provide connectivity between city facilities, Mackle Park, a racquet center, the library and a new museum. The trail would include bicycle and pedestrian enhancements. Ultimately, it would provide a safe means of transportation especially for children who frequent Mackle Park.
The $400,000 Gordon River Greenway request for enhancement funds would have built boardwalks along the river in downtown Naples. The total cost of the Gordon River project is estimated at $14.1 million.
"In my opinion, the sidewalks and safety trumps recreation," Naples Councilwoman and MPO member Dee Sulick said during Friday's meeting.
Over the next two years, the county will receive about $800,000 in federal enhancement funding. The funds, which must pass through FDOT, are made available for projects that expand transportation choices and enhance the transportation experience.
Currently, there is a tremendous amount of traffic around the Mackle Park area, said Marco Island City Council Chairman William Trotter, who serves on the MPO.
With the addition of the off-street trail, Trotter said a lot of foot traffic would be taken off the road.
Collier Commissioner Donna Fiala motioned to make the Marco Island linear park trail project, which would cost $209,000 to build, to the top ranking.
The MPO voted 4-3 to approve the proposed changes to the transportation enhancement projects list.
The Gordon River park fell to fourth on the priority list.
"I'm disappointed," Naples Mayor Bill Barnett said, adding that the Gordon River Greenway Park has been on the drawing board for years.
FDOT asked the MPO to rank the enhancement priorities, but not based on cost.
"It gives the department and the MPO a bit more flexibility using funding for enhancements that fiscal year at the MPO's choice," said Debbie Tower, spokeswoman for FDOT.
Chairman Frank Halas questioned if there was a possibility of additional funding for the projects.
FDOT District 1 Secretary Stan Cann said he wasn't aware of additional funding.
However, four of the projects could qualify for FDOT's stimulus funding.Those include the sidewalks at three schools and the Marco Island linear park trail, according to officials.
The county expects to get $4.7 million from the federal government for infrastructure improvements. About $40.4 million would go to enhancement projects throughout the state.
The rest of Collier County's top five priorities:
2. Gulfview Middle School sidewalk- a Safe Routed Schools Project. Estimated cost of construction is $383,000.
3. Lake Park Elementary School sidewalk- a Safe Routed Schools Project. Construction cost is $218,000.
5. Riverside Filter Marsh Project, which includes re-routing water flows to the south and east through a filter marsh pump station. Construction cost is $200,000.
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Add big style to your smaller powder room with the Fresca Grazioso Modern Bathroom Vanity, model FVN3337ES. Dramatic styling, a dark espresso finish and a bold glass sink and countertop combine with a waterfall-effect chrome and glass faucet and a matching mirror to enhance your decor. A two-door cabinet with chrome-accented handles offers storage. A compact design makes this free-standing bathroom vanity with mirror a good fit for any space.
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The Binding of Isaac
Binding of Isaac: Rebirth hits Xbox One, Wii U, and New 3DS July 23
Poo and tears for everyone!
Jul 07
// Zack Furniss
Rejoice, fans of fetuses and feces! The Binding of Isaac: Rebirth will be coming to the Xbox One, Wii U, and New 3DS on July 23. Please note the new there, as it will not be available on the older model. It's wonder...
Rebirth alone
The Binding of Isaac: Rebirth 3DS lacks local multiplayer, cross-platform saves
Will be $15
Jun 12
// Jed Whitaker
Local co-op will not be available on the New 3DS version of The Binding of Isaac: Rebirth, confirms Tyrone Rodriguez, the founder of Nicalis. Tyrone also notes that patching it in after release is unlikely. Cross save is also...
Is Nicalis teasing more 1001 Spikes or something new?
1001, 1002, 1003...
Jun 08
// Zack Furniss
[Update: A little digging on Twitter shows us that Tyrone Rodriguez from Nicalis and Samu Wosada of 8Bits Fantatics have been exchanging ninja graphics for a little while now. Samu also released a free game called Goody Bad H...
The Binding of Isaac
New boss teased for The Binding of Isaac: Rebirth expansion
Rag n' Bone
Jun 03
// Zack Furniss
The Binding of Isaac: Rebirth's expansion, Afterbirth, is shambling ever-closer. Yesterday, Tyrone Rodriguez of Nicalis teased a new boss, Rag Man. This mummy-lookin' homie has purple eyes, purple attacks, and the abilit...
Top 5: Trending stories
Updated every 15 minutes
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The Binding of Isaac
The Binding of Isaac: Afterbirth to have daily challenges
Now I can suck daily
May 27
// Zack Furniss
In the months since November 2014, I've slowly developed some semblance of skill in The Binding of Isaac: Rebirth. While I've still got quite a lot of playing to do if I wish to earn the Platinum God achievement, I'm impresse...
1001 Spikes
1001 Spikes Wii U patch offers new control options and Off-TV play
...but it has some issues
May 24
// Jonathan Holmes
Last April, Nicalis announced that I'll be a character in 1001 Spikes someday. As you might imagine, that announcement has changed the way my brain works. "Is today the day that I get to play as myself alongside Jonathan Blow...
The Binding of Isaac
Check out The Binding of Isaac: Rebirth on the Wii U GamePad
23 glorious seconds
Apr 29
// Zack Furniss
When The Binding of Isaac: Rebirth came out last November, I got sucked back into the dark, dank basement full of doo-doo for a good two months. This video of Isaac on a Wii U GamePad is enough to get me thirs...
Afterbirth update
The Binding of Isaac: Rebirth expansion to feature new transformations, ruin my life
Norman Bates chic
Apr 28
// Nic Rowen
Well I'm doomed. The Binding of Isaac: Rebirth expansion, Afterbirth, is going to include a whopping eight new transformations to discover, experiment with, and obsess over. I'm already addicted to transforming into Guppy the...
Isaac shirts
Bind yourself in Isaac shirts
Show your naked crying boy pride
Mar 22
// Nic Rowen
These Binding of Isaac shirts from theyetee.com combine the terror of Isaac with nostalgic love for Zelda, creating something that is making me reconsider my "no game t-shit" policy. The three new shirts include a...
Castle in the Darkness
Beard View: Castle in the Darkness
'Maybe I'm just good and you're not'
Mar 09
// Jed Whitaker
This weekend I was at PAX East and had been busy shooting some videos with our lovely bearded editor-in-chief when this was uploaded, so in case you missed it, check out the latest Beard View in which I show off one of my fa...
Beard View Live
Watch me die in Castle in the Darkness on Beard View Live
NES style Metroidvania greatness
Mar 01
// Jed Whitaker
Hey Beard View fans, I'll be playing Castle in the Darkness over at our Twitch channel at 6:30 pm Eastern / 3:30 pm Pacific. You can obviously watch it embedded below, but I encourage to come join the chat and keep me co...
Nicalis platformer
Ready for punishment? Castle in the Darkness awaits
Precious coins ease the pain
Feb 05
// Jordan Devore
I think I'm as ready as I'll ever be for Castle in the Darkness. It's out today for PC from publisher Nicalis and developer Matt Kap, who was also the lead artist on The Binding of Isaac: Rebirth. This super-challenging adven...
Cave Story
Cave Story 2 folder spotted on Nicalis desktop at PAX South
Could mean nothing, or it could mean everything
Jan 24
// Jonathan Holmes
PAX South is going on right now, and two of our most honey-loving sugarbears are in the fray as we speak, lapping up all that sweet golden joy. One of the games they're set to check out is Nicalis's Castle in the Darkness, a ...
Castle in the Darkness leaps to PC on February 5
Nicalis' forthcoming platformer hits Steam next month
Jan 22
// Kyle MacGregor
Castle in the Darkness, the next ultra-difficult platformer from Cave Story and 1001 Spikes publisher Nicalis, is poised to debut via Steam on February 5. The project was developed by Matt Kap, the lead artist behind Th...
More Binding of Isaac
Fan ideas will make it into The Binding of Isaac: Rebirth's expansion
A new mode will 'almost double the amount of things you can do'
Nov 25
// Jordan Devore
As a fan of The Binding of Isaac, I had lofty expectations for Rebirth and it more than delivered. Problem is, I don't have enough time to play it (and make any real headway). And now there's more content on the way. Like the...
We played Binding of Isaac: Rebirth because we haven't had a good cry in a while
Nov 19
// Bill Zoeker
Who beats off on paper? Werewolves. That's who.
Max and I started playing The Binding of Isaac: Rebirth because it's a good-ass game, and we like to do gaming. Max has actually never played any iteration of Binding of Isaac before, so I let him go first, knowing that I'd only have to wait a few minutes before my turn.
Review: The Binding of Isaac: Rebirth
Nov 11
// Nic Rowen
The Binding of Isaac: Rebirth (PC [reviewed], PS4, Vita)Developer: Nicalis Inc, Edmund McMillenPublisher: Nicalis, IncReleased: November 4, 2014MSRP:$14.99 Everything about Binding of Isaac is crazy and feels like it somehow shouldn't exist. But it does, and there is a kind of naughty thrill in that, like getting away with a rude joke at the dinner table. For those unfamiliar with the original, Isaac is a roguelike twin-stick shooter/dungeon crawler where you play as a small naked boy named Isaac, whose mother, believing she has heard the commanding voice of God, is trying to kill him. He desperately flees into the basement where he encounters deadly insects, mutated siblings, and every sort of grotesquery you could care to imagine. He fights them with his tears. Yup. Every run is a new experience, a single chance at heaven or hell. If you die, it's back to scratch. The game is deeply mysterious, featuring no fewer than 16 different ending, at least 8 unlockable alternate characters, 4 bonus levels, and literally hundreds of items to discover, unlock, and experiment with. It's a deep, dark rabbit hole and if you have any kind of completionist streak it will ruin your life (take it from me). Isaac may present itself in the familiar sheep's clothes of a Zelda clone, a fun romp through some old-school top-down action, but that's just the skin-suit pulled tight around the beast. At its heart, Isaac is its own twisted, beautiful monster. The most notable difference between Rebirth and the original Isaac is the shiny new engine and graphical overhaul. The original was made in Flash, giving the game a distinctly smooth and cartoony look, and with no shortage of technical problems to boot. Rebirth ditches Flash (and thankfully all of the bugs and glitches associated with it), dropping the smooth lines for a more detailed, SNES-era pixelated look. More importantly than the faux 16-bit trappings, the new engine allows for a smoother and more stable experience. Where the frame rate of the original would drop through floor like a bowling ball when too many shots or enemies got on the screen, that's no longer an issue. Rebirth runs at a flawless 60 FPS come hell or high water. With the smoother graphics come some gameplay changes. Rebirth is a much more shooty (to use my highly technical vocabulary) game than fans might be used to. The dependable frame rate allows for much more intense fights than the original ever dared to attempt, veering into bullet hell shmup territory on occasion. All of the new bosses introduced in Rebirth (and there are a lot of them) are much faster and aggressive than the old guard, and some of the returning enemies, particularly the final bosses, have been overhauled to be FAR more trigger happy than they used to be. I never thought I'd be happy to run into the likes of Loki or Peep, but I'll take them any day over the new recruits in Satan's army. Thankfully, bullet hell is a knife that cuts both ways. Rebirth does not shy away from crazy item combinations that completely break the game. At one point I had a fully upgraded rate of fire with floating anti-gravity tears mixed with ricocheting rubber cement and a boomerang effect. I would step into a room, hold down the fire button for about a second or two, let go, and watch the entire room be enveloped in tears and instantly eradicate everything. The game might have gotten more difficult, but there are also more items to help turn it around. Along with the other technical advancements, there is more variety in the shape and size of the dungeon chambers in Rebirth. Rooms are no longer limited to the single screen rectangular format they used to be. You will come across long hallways rigged with traps, huge arenas filled with enemies, big multi-screen affairs that will scroll along with Isaac's movement. These massive rooms have hosted some of the most intense moments I've had with Rebirth, the added space allowing for multiple mini-boss battles or elaborate traps. Rebirth includes a small, but delightful, two-player mode. At any time player two can join in as a tiny, floating ghost baby at the cost of one of player-one's heart containers. Ghost baby is definitely second fiddle, unable to plant bombs, walk through doors, or pick up items (no cheating and grabbing something from across a gap), but he will benefit from whatever kind of shot upgrade Isaac has collected. It won't become the new way to play Binding of Isaac any time soon, but having a wingman is loads more fun than it has any right to be. Rounding out the new additions are a few quality of life tweaks. Control pads are now supported on the PC version, and work perfectly fine if that's your preferred style. You can choose between "Normal" and "Hard" modes now, letting you somewhat regulate what brand of insanity you're looking for. Hard mode, of course, hides its own set of exclusive items and enemies, so anyone looking to collect all the goodies should prepare to suffer for the compulsion. Rebirth graciously now allows you to quit mid-way and return later, instead of holding you hostage to a good run. ("I'm going to be late but I have fully powered tears and twelve heart containers! I mean, the divorce rate around here is like 55%, so I can probably get another shot at being my brother's best man, right?"). It is potentially a life-saving addition for the truly possessed. Rebirth surfaces the randomized "seed" of each run, a small series of numbers you can input to generate the same map/item pick-ups. You can replay particularly great runs, near misses, or swap favorable map layouts with friends. This is a shockingly generous addition that seems to run counter to much of the game's otherwise unforgiving and hard-nose posture. It seems so out of place that the idea rankled me. I respect the purity of the one-chance, perma-death run. Watering it down with de facto do-overs cheapened the experience for me faster than fall of the Berlin Wall devalued the Soviet Ruble. As an addict of the first game, my favorite enhancement is also the least consequential, Rebirth is able to visually stack multiple items on Isaac with fewer conflicts than before. It may seem silly, but watching Isaac's strange transformation from a tiny naked boy to a pustulating, winged, blood trailing monster, or failed Siamese twin with a chemical burn, or lipstick wearing cyclops being followed around by the floating head of his dead cat, or whatever, is one of the greatest pleasures in the game to me. My heart always broke a tiny little bit when one item would overwrite another, or just not appear at all. I love just about everything about Rebirth and (if you couldn't already tell) I can't recommend the game enough. But I feel like I would be remiss if I didn't talk about the poop. There is a lot of poop in this game, a lot of tasteless dead baby jokes, gross out gags, and weird Christian imagery, all of which might rub some people the wrong way. Personally, I don't mind that stuff and I think the game earns some of it's nastiness (the very core of Isaac is a sad story of child abuse as seen through the eyes of the child experiencing it after all, it's going to be horrifying and juvenile), rather than just being gross for grossness sake. Still, it's going to be a deal breaker for some people. The Binding of Isaac: Rebirth is hands down the best version of Isaac. It improves upon the original, a fantastic game in its own right, in every conceivable way. If you haven't played Isaac yet, this is the version to get. If you are a fan of the original, these are so many new items, enemies, and experiences to be found in Rebirth that it feels far more like a sequel than a remake. Rebirth is an incredible experience that can't be missed. Descend into the basement, lock the trapdoor behind you, and don't look back.
The best game you'll ever play about washing poop out of a basement with your tears
In 2011, I lost a chunk of my life. An insidious tendril of addiction, despair, and obsession caught me by the ankle and dragged me into the The Binding of Isaac's darkened basement. I lost dozens of hours, whole days at a ti...
Wii U
The Binding of Isaac: Rebirth teased for Wii U
Smiley face
Oct 07
// Jordan Devore
Fans of The Binding of Isaac have long wanted the game on 3DS and while that'd be great to see one day, I'd be just as happy to to have it on Wii U. Which might actually happen! I know! Nicalis uploaded this video yesterday and that blip at the beginning sure is a Wii U overlay. There's also this Twitter exchange between designer Edmund McMillen and Nicalis' Tyrone Rodriguez. [Via GoNintendo]
Nicalis kicked my ass again with Castle in the Darkness
Sep 10
// Jordan Devore
Let's get down to business
After checking out The Binding of Isaac: Rebirth at PAX Prime, I spent a decent chunk of time with another upcoming Nicalis project, Castle in the Darkness. It's a challenging platform-adventure PC game that feels all too app...
Binding of Isaac
The Binding of Isaac: Rebirth is everything I hoped it'd be
PS Vita owners especially are in for a treat
Sep 08
// Jordan Devore
One of my favorite parts of PAX Prime this year was an appointment with Nicalis. Not only was the meeting away from the crowded, noisy Washington State Convention Center, it was an opportunity to get sucked into The Binding o...
Binding of Isaac
Co-op in The Binding of Isaac: Rebirth is looking good
A short Let's Play preview from the McMillens
Aug 19
// Jordan Devore
Here's Edmund and Danielle McMillen previewing cooperative play in The Binding of Isaac: Rebirth including one of the game's twenty challenges, Head Trauma, in which you have tons of tears spraying all over the place but the...
Nicalis announces Castle in the Darkness for PC
Coming to Steam this summer
Aug 06
// Kyle MacGregor
Castle in the Darkness is leaping to Windows PC this summer, Nicalis announced today. The retro-style platformer draws inspiration from "the golden era of videogames," vaunting an expansive open-world and a "damning" difficulty curve. The story follows a kingdom's last remaining royal guard on a mission to save the land from a sorcerer and his evil army.
Binding of Isaac
Replay runs, customize controls in The Binding of Isaac: Rebirth
PS Vita visuals won't be exactly on par
Jul 29
// Jordan Devore
I convinced myself long ago that The Binding of Isaac: Rebirth was something I needed in my life, so every additional detail or feature we're told about in the lead up to release is just icing on top. This week, customizable ...
Another fair comparison: 1001 Spikes is better than Game of Thrones
Jul 05
// Jonathan Holmes
Death with a purpose Game of Thrones and 1001 Spikes are known for their surprise deaths. In Game of Thrones, these deaths usually manifest by witnessing one or more characters who were once pivotal to the larger narrative being maimed or murdered in a graphic and disturbing fashion. In 1001 Spikes, death usually pops up in the the form of a sharp object suddenly piercing into your flesh, resulting in an adorable and gruesome spray of blood-red pixels. Both types of deaths work to send the message that no one is ever safe. That's all well and good, but that message in and of itself doesn't say anything particularly novel. The world is a hostile place filled with lethal problems. We're all going to die. No one is safe. Water is wet. Hamburgers are not actually made of ham. These are all truths, but telling the truth is not in itself very compelling. There needs to be a message beyond the gimmick, an idea behind the violence for it to hold any lasting emotional resonance. Sadly, Game of Thrones doesn't manage to pull that off. Like so many other aspects of the show, its deaths are dressed up to appear sophisticated and meaningful but are really just softcore porn, in this case, of the gore-porn variety. After the shock and awe has passed, the drama continues, with no lessons learned or philosophies shared. In 1001 Spikes, every death is a lesson. Every surprise stabbing is an opportunity for growth. Those who start the game with a feeling of dread and hopelessness may learn, through many hardships, that they are smarter, stronger, and more capable of survival than they would have ever thought possible. Game of Thrones is a largely nihilistic one-note tune, where even the survivors end up emotionally/ethically dead on the inside in no time flat. 1001 Spikes is a celebration of the negative space that death offers to our mortal experience, and how it shapes every moment of brilliance, inspiration, and virtual athleticism that brings life to its fullest. Escape from the victim/victimizer/protector triangle You know what's hot in entertainment right now? Trauma and victimization. The Walking Dead, Orange is the New Black, 24, and of course Game of Thrones all chronicle the experiences of those in a constant state of fight-or-flight response. Our society is currently fascinated with witnessing what lies beneath society's veneer of civility and compassion. Why are we so obsessed with turning over the rocks of the human soul and seeing how gross the bugs are underneath? I'm guessing it's because we're living in the least safe time in human history. Everyone is spying on everyone. We can't go a month without hearing about another young man violently attacking a group of strangers. Drones are in our air space. Terrorists are in our news feed. Hackers are stealing our credit card information. Everyone is at everyone on Twitter/Facebook/Tinder and we're all thinking about how stupid, fat, and ugly everyone else is. When people are under threat, their minds naturally shift towards grouping everything around them into one of three categories: the victims, the victimizers, and the protectors. You see this all the time online. People take on victim posture in order to drum up sympathy and entitlement to attention and care, or abuse and threaten each other to give themselves the illusion of power and safety, or lash out at others that they deem to be victimizers, granting themselves entitlement to be as destructive and hostile as they want "for the greater good." This is exactly what we see happening in shows like Game of Thrones. Every character is moving around within this triangle, taking on victim, victimizer, or protector posture depending on what works best for them at the moment. The question is, where does it get anyone? Either dead, angry, alone, or evil. Those are the only options for an endgame in the victim/victimizer/protector structure. There is no room for empathy in that triangle. There's no room for enlightenment. There's only room for conflict. There is another way, though. There is a road out of the triangle's trap. There is the path of the victor. This is the path that 1001 Spikes presents us with. In 1001 Spikes, the environment is the victimizer, and Aban is the victim. In time, he learns to be the protector, actively fighting back against the forces working to render him powerless. That's not the end though. As any 1001 Spikes expert knows, truly mastering one of the game's levels doesn't feel like a fight. It feels like a conversation. The developer is communicating through their level design, and the player communicate back through their actions, and the back and forth gradually transforms from an angry screaming match to a beautiful duet. To get to know and love the developer through surviving, and eventually then thriving, the world of challenges that they created with you feels a lot like love. When you're able to elude every trap, make every jump, hit every note with pitch-perfect accuracy, you join with the developer in a way that the Spice Girls once sang about. That's not just surviving. That's living. That's living life right. A hunger that leads to improved nutrition Most of the aspects of Game of Thrones and 1001 Spikes affect us on an deep, guttural level. Human beings are suckers for the guttural. We spend so much time being self conscious, or worried about our futures, or obsessing about mistakes we made in the past that when something can help us turn off our brains for a while and grab us by the balls (or nipples, or whatever), it's intoxicating. Game of Thrones grabs our balls in multiple ways. Sex, violence, taboos, mythologies, drama -- all things that capture our attention on primitive level. But what do they offer us for emotional/psychological sustenance? Sadly, not much. If you're lucky, the story may help you to conceptualize and gain insight into your own relationship problems. Through witnessing the ugliness on display in Game of Thrones, you may become more aware of the ugliness in your own life. How is that fun? It's not, and it's also not why most people watch Game of Thrones. It's because it hooks them in a world of constant negative stimulus, constant emptiness and pain, and dangles a carrot in their faces promising that things might get better, that they might gain some emotional sustenance if they watch just one more episode. Stories like Game of Thrones (and certain videogames) are designed like sour candy. They start off sweet, with fun stuff like sex, intrigue, and power fantasy, but leave you hurting, with the sour taste of trauma and sorrow in your mouth. That sour leaves you hungry for an antidote to cancel out the sour, something like the sweet sex/power/intrigue that got you watching the show in the first place. So the cycle of sweetness, sourness continues, leaving the audience continually hungry and never truly fed. 1001 Spikes grabs you by these instincts as well, but it's all in the service of feeding you something real. 1001 Spikes and Game of Thrones both inspire a similar morbid curiosity for how bad things can possibly get next, but where Game of Thrones leaves you feeling like the world and everyone in it are horrible, 1001 Spikes inspires you to believe that you (and every one else) are capable of anything. Game of Thrones is a syrupy, acidic, sweet and sour soup made from anger, hopelessness, and nihilism. 1001 Spikes is a bitter but hearty broth that inspires patience, problem solving, and the knowledge that as long as you believe in yourself, anything is possible. So if you've got some internalized victim/victimizer/protector issues that you'd like to work out externally, stay away from Game of Thrones. It will only work to reinforce that maladaptive power dynamic and leave you hollow inside. Instead, try 1001 Spikes. While it's not as easy to digest as a show that invites you to sit back and passively watch as the atrocities unfold before you, it's well worth the effort if you're at all interested in becoming a more psychologically well-aligned and resilient person.
Ukampa > Westeros
Believe it or not, there once was a time when many, many people thought that Call of Duty: Advanced Warfare was better than Advance Wars. Thankfully, those days are over now. According to my numbers, 89.6% of those who o...
Speed Runs
Skyward Sword and 1001 Spikes speed runs will get your blood pumping
Glitch assisted or not, the athleticism shines through
Jun 28
// Jonathan Holmes
Skyward Sword and 1001 Spikes may not look like they have a lot in common, but they share a lot of the same design sensibilities. Both games are about exploring relatively small, densely packed areas that appear simple on th...
1001 Spikes
1001 Spikes leaps to Xbox One next week
Prepare to die. A lot.
Jun 07
// Kyle MacGregor
1001 Spikes is landing on Xbox One next week, publisher Nicalis recently announced. The insanely difficult platformer is already available on PlayStation 4, Vita, Steam, Nintendo 3DS, and Wii U, but Xbox One owners will ...
1001 Spikes
1001 Spikes knows how to do a launch trailer right
Plus footage of the 3DS version
Jun 03
// Jordan Devore
Ooh, this is looking good. In the lead up to 1001 Spikes, there's been plenty of footage shared but much of it appeared to be from the earlier levels. Now, with this launch trailer, we've got a proper preview of the misery t...
1001 Spikes
1001 Spikes also hitting Wii U, 3DS on June 3
May 30
// Jordan Devore
We heard the PS4, PS Vita, and Steam versions of 1001 Spikes were coming June 3. Which is great and all, but I know a number of us were going to hold out for the game on Wii U or 3DS -- no need to! Nicalis has confirmed those...
1001 Spikes comes to PS4, Vita, Steam on June 3
Go die
May 28
// Dale North
Nicalis' 1001 Spikes is a game I'm excited about but have somehow completely missed the chance to talk about. It's a particularly brutal action-platformer that also doubles as an outstanding Mario Bros. style party game. Ther...
Toribash on Steam
Turn-based fighting game Toribash now available on Steam
Or maybe 'Toriflail,' as I would play it
May 20
// Darren Nakamura
Do you know Toribash? It has existed as a free-to-play game for a while now on Mac and PC, and it even came to WiiWare several years ago. The turn-based, muscle micromanagement fighter starring Vectorman's cousins may now ga...
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So, I plan on using my GameStop Power-Up Rewards points (yes) to buy all the eventual P4DAN DLC. Even though the practices are very suspect. I am so not made of stone. Moreso blubber and Dr. Pepper.
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Pixie The Fairy
A Nintendo rep gave this to me today because she likes talking to me. I do have lots of the Old AC eReader cards I guess I could put in it... [img]http://i.imgur.com/KFvoFvWl.jpg[/img]
I'm still trying to figure out how to do it, but for the most part I like what I've seen of the new blog editor. My first blog post using it should go up tomorrow.
Pixie The Fairy
The demon threat is real.[img]http://www.destructoid.com//ul/313317-those-meddling-kids-persona-4-jumping-all-sharks/2015-10-03-180913.jpg[/img]
Mike Martin
Daily VGM #20 (Bonus) - Valak Mountain Night (Xenoblade Chronicles) [youtube]http://youtu.be/roRsBf_kQps[/youtube] This theme perfectly captures the essence of Valak Mountain. It feel serene and calm, as well as mountain-esque (lol).
What does one do when MGS V has lost its luster and Persona 4: DAN is growing stale? Listen to Queens of the Stone Age, of course. And wonder; is Steins;Gate worth the asking price? I want it, but not sure if I $30 - $40 want it...
Terry 309
Been trying to get Tales Of Phantasia finished before Zestiria comes out... How i'm going to get through that game I have no clue... especially when Exist Archive comes out in December and I have to play that too... also i've still yet to finish Grandia 2
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text:
Ck Be Cologne
By Calvin Klein for Men
Ck Be Cologne by Calvin Klein, Launched by the design house of calvin klein in 1996, ck be is classified as a refreshing, oriental, woody fragrance . This unisex scent possesses a blend of musk, mandarin, magnolia, peach and sandalwood. It is recommended for daytime wear. All products are original, authentic name brands. We do not sell knockoffs or imitations. ... Read More
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About Calvin Klein
History and background of Calvin Klein. A pioneer of designer jeans, underwear and the wholesome, all-American look, Calvin Klein is one of the fashion world's biggest names - and brands. Born in 1942, Calvin Klein taught himself how to sketch and sew as a boy. He went on to win a place at New York's High School of Art and Design and the Fashion Institute of Technology and finally launched his own label, in partnership with childhood Read more about Calvin Klein
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It is a good fragranc
I usually put 3 sprays a day and it works good but it dries out after about 2-3 hours probably because i use it during the winter, I have yet to try it...Read complete review
I usually put 3 sprays a day and it works good but it dries out after about 2-3 hours probably because i use it during the winter, I have yet to try it out during the summer. I love the smell of this! This is the first fragrance i've ever bought. The only thing is that it doesn't stay for long other than that its really good for a daily use and the smell is great
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Different gift option
I bought this perfume to give to a friend as a gift, but to be honest with you guys; this is not my favorite fragrance and it doesn't hold like the other ones I...Read complete review
I bought this perfume to give to a friend as a gift, but to be honest with you guys; this is not my favorite fragrance and it doesn't hold like the other ones I like to use!
Reviewed by 92 customers
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- Always Reliable
- Amazing Prices
Best Uses
Comments about Calvin Klein Ck Be Cologne:
I have been buying my perfume from this site for over a year now and I have never been dissapointed. The prices are the best I have found anywhere online or in stores. I tell everyone about this site and how amazing it is! I very much recommend his site to everyone.
- Fragrance Type:
- Ck Be
- Clean & Fresh
- Great Smell
- Sensual
- Sexy
- Too Expensive
Best Uses
- Daily Use
Comments about Calvin Klein Ck Be Cologne:
great fregrence
- Fragrance Type:
- Citrus/Fruity, Oriental/Spicy
- Great Smell
- Great Value
- Sensual
Best Uses
- Anytime
Comments about Calvin Klein Ck Be Cologne:
For every time usage
- Doesn't Hold
- Too Weak
Best Uses
Comments about Calvin Klein Ck Be Cologne:
It used to be a good product that i wore all the time.Now,it is just like water with slight aroma to it.I don't know if it manufacture's fault or the seller's but i will never buy it again...
- Clean & Fresh
- Draws Compliments
- Great Smell
- Pricey / Poor Value
- Too Expensive
Best Uses
- Anytime
Comments about Calvin Klein Ck Be Cologne:
this is a very nice, refreshing scent, not overpowering. doesn't grow with time like so many colognes do.
- Fragrance Type:
- Citrus/Fruity, Woody/Earthy
Displaying reviews 1-5
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text:
New Jersey Gov. Chris Christie (R) and former Secretary of State Hillary Clinton (D) are the two most favorable, or "hottest," politicians in a new national survey.
Christie topped the list with a 53.1 rating on the survey's "feeling thermometer" while Clinton scored a 52.1.
Quinnipiac University Polling measured the public's receptiveness to a number of political leaders using a scale from zero to 100 with the higher number representing "the warmer or more favorable" voters felt about a lawmaker.
However, Christie, ranks eighth among Republicans with a score of 59.8, behind a host of other potential GOP presidential contenders. The New Jersey governor leads among Independents.
Paul Ryan tops the list of Republicans with a 68.7 rating.
A Pew poll last week found Christie's favorability rating trailing with the GOP as well.
Two dark horses to score high on the list are freshman Sen. Elizabeth Warren (D-Mass.) who ranked third and Sen. Kirsten Gillibrand (D-N.Y.) who ranked fifth.
President Obama ranked fourth with a 47.6 score. Obama's rating has dropped nearly 10 points since a similar survey was taken in March 2011.
Unsurprisingly, the Democratic and Republican leaders in Congress bottomed out on the list, with Senate Majority Leader Harry Reid (D-Nev.) coming in last (33.8).
In other surveys, Congress's approval rating hangs in the low double digits.
The poll surveyed 1,468 national registered voters and has a margin of error of 2.6 percent.


text:
TUCSON (KGUN9-TV) - The pride behind the University of Arizona football team, the marching band, is being left out of the affair this year.
The Advocare V100 bowl game in Shreveport, La. is not PAC-12 affiliated nor close to Arizona, and band director Jay Rees said cost and travel played a huge factor in the decision.
When the band and athletic department originally looked into flying the Pride of Arizona to the bowl game, Rees said flights alone could cost upwards of $300,000, not to mention the cost of hotel and food for 250 band members.
He said taking buses had its own challenges, like a 6-day round trip more than 1,100 miles away for a 2-day event.
But even without the Pride of Arizona, Rees said that in an unusual twist you'll only see in the music world, Wildcat fans will still get to sing along and jump around to "Bear Down."
The Northwestern State University's marching band, a college in Louisiana, will play "Bear Down" and "All Hail Arizona," ditching their traditional purple for two days and doning cardinal red and blue.
The UA is paying for the band's cost and time, which is a much cheaper price tag than if the "Pride" were to march to Louisiana.
Rees said the band has not missed a bowl game since 1990 when the Wildcats played in the Aloha bowl in Hawaii. In that game, Syracuse shut out Arizona 28-0.
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