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Select Local Merchants
Valley Social Dance StudioRead MoreCheck out the amazing schedule at Valley Social Dance Studio for a list of the best shows Menasha offers. Parking is plentiful, so guests can feel free to bring their vehicles.167 Main StreetMenasha, WI US
Educational DesignersRead MoreShop laptops, computer parts, printer_copier_scanner_string, and desktop computers and other great computers and go home with a new and reliable computer you can count on. With the many parking choices near Educational Designers, finding parking within walking distance is a breeze.N3608 State Road 110Weyauwega, WI US
Brain BalanceRead MoreSeek alternative medical treatment from the specialists at Mequon's Brain Balance. Offering a number of alternative services, such as homeopathy and naturopathic therapies, this clinic allows you to choose your preferred method of care. The alternative methods used by the doctors at Brain Balance have produced great results in their patients. Call to find out more.11649 North Westport CircleMequon, WI US
Mathnasium - The Math Learning CenterRead MoreMore than three decades ago, educator Larry Martinek set out on a mission to develop a curriculum that would radically change the traditional approach to teaching math. Noting a "disconnect between students' basic skills training and the curriculum they [must] master in the years to come," Larry created an original teaching method designed to turn students into miniature mathematicians capable of thinking critically to solve problems. His approach, which he describes as the cultivation of number sense, strives to sharpen students' math instincts, rather than drill them with repetitive, memory-based exercises or force them to blackmail accountants to crunch the numbers. Soon after students began using Larry's method, their test scores began to rise. In the spring of 2002, Larry's dream came true. Peter Markovitz and David Ullendorff, leaders in the education industry, made Larry and his curriculum the driving force of Mathnasium. Larry introduced his curriculum as the Mathnasium Method. Today, Mathnasium centers can be found throughout the world. Informed by Larry's visionary innovations, the program's tutors give personalized coaching that focuses on bolstering critical thinking through written materials and mental math, forsaking many of the teaching tools found in a traditional classroom. In addition, the tutors also focus on boosting students' enthusiasm for the subject, helping them overcome a lack of confidence in the classroom or their innate fear of prime numbers.N78W14569 Appleton AveMenomonee Falls, WI US
Hands On Art StudioRead MoreTreat your eyes to some world-class art at Fish Creek's Hands On Art Studio. Check out the restaurant at this museum for a delicious meal. Tots are more than welcome to hang with their parents at this museum. Parking is plentiful, so guests can feel free to bring their vehicles.3655 Peninsula Players RoadFish Creek, WI US
Northland Baptist Bible CollegeRead MoreThere are so many cultures in the world, each with its own beauty and distinct traditions. Experience something culturally new at Northland Baptist Bible College in Dunbar. Parking is plentiful, so patrons can feel free to bring their vehicles.W10085 Pike Plains RdDunbar, WI US


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SEATTLE, Dec. 2 /PRNewswire/ -- A "challenge donation" appeal at last night's Hutch Holiday Gala helped push the total funds raised to more than $4.2 million for Fred Hutchinson Cancer Research Center.
The annual black-tie Gala is the largest fundraiser for the Hutchinson Center. The event took place from 5 p.m. until midnight at the Seattle Sheraton Hotel.
"Help the Hutch" is the portion of the live auction during which every dollar donated is matched by challenge donors. One of those donors was Susie Morganti of Bellevue, Wash., who lost her husband, Johnny, to cancer last summer after 73 years of marriage. Following Johnny's death, Susie decided to donate $500,000 in memory of her husband and a late niece, Jackie Bylund. Morganti, who yesterday celebrated her 95th birthday, said she was inspired by the prospect of leveraging more donations for cancer research.
"We were pleased to present the Hutch Holiday Gala challenge in honor of loved ones we've lost," said Gary Bylund, Morganti's nephew and family spokesperson. "In one way or another, most of us have experienced life changes because of cancer. The Hutch Holiday Gala is our opportunity to positively impact cancer research at the Center, and thereby the lives of our family and friends."
Dr. Lee Hartwell, president and director of the Hutchinson Center, said donations such as Morganti's are essential to help Center scientists make discoveries that improve the future of human health.
"We are so fortunate that our guests recognize the power of private support in the fight against cancer," Hartwell said. "Through the Hutch Holiday Gala, we partner with these generous families and others in the community to empower our science against cancer and other diseases."
Gala patrons also opened their pocketbooks for lavish live-auction
packages such as dinner for 20 with the Hutchinson Center's three Nobel
laureates, which sold for $26,000; a Lake Union cruise and scavenger h
|SOURCE Fred Hutchinson Cancer Research Center
Copyright©2007 PR Newswire.
All rights reserved

subset: commoncrawl


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All posts by Garyclevewilson
We Wanted a Test Tube Baby
The doctors warned us that it wouldn't be easy. We didn't know how right they were.
The odds for having a child of our own were slim to none. We took a chance on slim.
Texas Primer: "Tex"
It's the best nickname you could have, even if you've never been to Texas.


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The free market occasionally annoys, but the government often makes matters worse. Consider the Americans who purchase a cellphone from the big providers such as Verizon, AT&T, T-Mobile and then regret it. Though most modern cellphones are capable of working on the networks of other providers, companies often lock up the devices so that a consumer can't make the switch to a competitor.
Americans being Americans, enterprising customers often figure out how to bypass these artificial constraints through a process called "jailbreaking." Jailbreaking software gives owners the power to install third-party apps, custom ringtones and other applications. To the delight of the phone companies, a recent decision by the Librarian of Congress, as empowered by Congress, cracked down on freedom by declaring jailbreaking a serious crime.
Congress in 1998 passed the Digital Millennium Copyright Act, which gives the librarian power to decide whether certain acts, such as unlocking a phone, should be considered a copyright violation. The recent ruling means consumers who free their phone without their carrier's consent can be fined up to $2,500. Willful violators could be sent to prison for five years and required to pay a $500,000 fine.
It's not the first time, and certainly won't be the last time, that corporate interests have used government as a means of limiting competition and padding their bottom lines. Big content industries have been lobbying Congress to restrict innovation and technology for generations. In the late 1970s, for instance, Hollywood went all the way to the Supreme Court in an effort to hobble the VCR, which was then the way we recorded the television programs and movies we wanted to watch later.
Passed by a Republican Congress in 1998 and signed into law by President Clinton, the Digital Millennium Copyright Act is a classic example of bipartisanship that favors corporate money interests at the expense of inventors, competition, technology and innovation. Movie studios, the music industry and technology conglomerates were chief advocates of the law, and Congress gave them what they wanted — the legal authority to create closed technology platforms, often forcing taxpayers to pay for enforcement of their copyrights.
Lawyers for these industries have used the law to limit consumer choices and stifle competition. For example, in 2001, a Russian programmer was arrested, accused of writing software for his employer that converted Adobe e-books to PDF. Hollywood sued and shut down a company that made software that enabled consumers to make backup copies of their own DVDs. Homeland Security officers have arrested students who modified their Xbox game consoles, and the 1998 copyright law has been used to block aftermarket competition for garage-door openers and laser-printer cartridges.
We can't count on reform soon. Hollywood's grip on Congress remains as tight as ever. Recently, a conservative staff member who had the temerity to write a memo advocating reasonable changes to our copyright statutes was summarily fired.
Whether it's jailbreaking an iPhone or making copies of purchased DVDs, the Digital Millennium Copyright Act has unbalanced the copyright system by according copyright holders complete control over all possible uses of a work — a proposition clearly counter to the intent of the Constitution, which created copyright as a "limited" right.
On Monday, the White House issued a statement agreeing the librarian's decision should be overturned. That should help advance the cause of unshackling innovation and technology from this misconstrued and misguided act.
The Washington Times


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Uomo Moschino
I have the posibility of buy one of these two fragrances very cheap for wearing those days that you want to use a comfortable and easy-to-wear fragrance...I think both have that style. Which one do you suggest me?
Thanks and happy new fragrant year!!!
Uomo Moschino
Yes, Moschino Uomo ?
Uomo, although I don't wear it nearly as much anymore. Nightflight is great, but very fleeting on my skin (2-3 hours tops)
The hunt for a Signature Fragrance is not an easy one! I see the light at the end of the tunnel! Edit: 2015... I've given up and given in
Want to buy or swap: A*men Ultra Zest, Bentley Intense, Mont Blanc Legend Intense, Paco Rabanne Invictus,
Albeit I fancy Nightflight, I have to vote for "Uomo?"
Funny I just bought a 3.4 oz of Nightflight for a whooping $15.00
Nightflight, clearly!


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So sorry, but only 4 items are allowed for comparison. I blame the gnomes and so should you.
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So sorry, but this item is sold out.
You probably would've looked amazing with it too.
We do have some similar items available right now though.
Check out Paddle BoardingView Other Similar Stuff
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Tuesday, June 6, 2006
© Copyright 2015 Gwinnett Daily Post
LAWRENCEVILLE - Police have decided not to charge a Lawrenceville homeowner who shot and injured an alleged burglar at his home last week.Detective S.K. Shaw of the Gwinnett County Police Department said Monday he will not take out any arrest warrants for 27-year-old Ronald Ramsey. The case file will be forwarded to Gwinnett County District Attorney Danny Porter's office for review. "There was no overt violation of criminal law the way we interpret the code section, but obviously Danny may be able to interpret it a little better than us," Shaw said. Ramsey had a valid permit to carry a concealed weapon, according to police. He could not be reached for comment Monday because his phone number is unlisted. Shaw said Ramsey had been staying with family members in order to avoid media that have attempted to contact him at home. Ramsey returned to his house on Oakland Walk Court May 30 to find a teenager in the process of burglarizing it. Ramsey's 3-year-old twins were in the car with him at the time, Shaw said. Police believe Ramsey grabbed a gun that was in his car and fired several shots at the suspect, later identified as 17-year-old Alrazi Basher, as the teen was walking toward a stolen Ford Expedition parked on the street. One of the bullets struck Basher in the abdomen and several others damaged the SUV. Despite being injured, Basher managed to climb into the Expedition and flee to a friend's townhouse several miles away. Residents of that townhouse on Waverly Hills Drive called 911 after they opened the door to find the wounded teenager on their doorstep. Basher is expected to be discharged from Gwinnett Medical Center in Lawrenceville some time this week, Shaw said. He has refused to be interviewed by police. Basher faces felony charges of burglary and theft by taking a motor vehicle. A complication with this case as it relates to self-defense laws in Georgia is that Ramsey shot the intruder in the front yard, not inside his home, Porter said. State law mandates that deadly force is justified only if entry to a home is made in a "violent and tumultuous manner for the purpose of assaulting someone in the dwelling, or if force is necessary to prevent the commission of a felony. "Of course I have not seen the reports or the statements, but the fact that the shooting took place outside of the home may complicate things," Porter said. Porter said he would ask for an expedited review of the case as soon as his office receives evidence from the Gwinnett County Police Department.
More like this story
- Police continue to investigate shooting ( June 1, 2006 )
- Burglar shot after owner comes home ( May 31, 2006 )
- Man apologizes in fatal shooting ( June 6, 2006 )
- 16-year-old girl wounded in bowling alley shooting ( June 7, 2006 )
- Two more charged with murder in 2005 case ( February 17, 2006 )


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Decennial Minerals Exploration Conferences (DMEC) was created in 2007 as the legal entity responsible for managing the Exploration 07 conference and is the corporation that now holds the copyright for the proceedings of the conference. The Exploration 07 conference, held in September 2007, was the fifth and most successful of a series of conferences which were held every 10 years since 1967. These meetings have been designed to summarize the advancements in the state of the art in minerals exploration technology over the previous decade through a series of focused technology reviews and case studies in the fields of geophysics, geochemistry, remote sensing and information processing and data management.
While the previous four decennial events were organized on an ad hoc basis by volunteers from within the Canadian provincial and federal governments, minerals exploration and service communities, the organizers of Exploration 07 were legally required to incorporate in order to properly manage an event of the size and complexity as the decennial conference. When Exploration 07 was officially finished in early 2008, with the distribution of the final conference DVD, the DMEC executive felt that the message conveyed by the Exploration 07 was of sufficient importance to the industry that more of an on-going presence within the exploration community was warranted.
The main purpose of DMEC is the planning, organizing and running of decennial conferences (the next one scheduled for September 2017). Additionally, DMEC will look for shorter term opportunities to promote its vision that the successful use of technology will most likely occur when technology is meaningfully integrated into programs and that this in turn, will most likely occur when the geoscientists involved are working in a collaborative fashion.
Interested in helping organize a DMEC event? Please contact one of the DMEC executives.


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Donyas HouseSeptember 17, 2011Home • United States
Persian WORLD
Featuring: retro parents, cooky Casey, killer kimmy, affirmative ally, shwastwd Shane, bragadotious boots, and daring Diego!
Who ever you are MAYNARD...I KNOW FOR A FACT THAT YOU ARE NOT AT BLAKES OFFICE OR MY CAR SO STOP CHECKING IN THERE. yours sincerely, THE REAL FUCKING MAYOR.
Casey and Shane you two are beautiful!!!! Have kids!


text: Evil Whisperers Tafsīr of Sūrah al-Nās by Shaykh Riyādh ul Haq Date: Friday 27th April 2012 Time: 8:30 PM Venue: Al Kawthar Academy, Leicester Sisters Entrance: 62 Gough Road, LE5 4AN. Brothers Entrance: Opposite Masjid Ali, 45-53 Smith Dorrien Road, LE5 4BF. Rightfully so, Muslims all over the world exert themselves in both listening to and reciting the Glorious Qur'ān. Indeed this is a commendable act, but another obligation is overlooked; that is, to understand the meaning and messages of the Qur'ān. In order to help fulfil this important obligation, every last Friday of this month, Shaykh Riyādh ul Haq will deliver a comprehensive discourse detailing the meanings, messages and themes of the Glorious Qur'ān. Understand the richness and deep meaning of each word and verse of the Glorious Qur'ān by attending these lessons of tafsīr. Shaykh will be continuing the commentary of the Glorious Qur'ān with Sūrah al-Nās on Friday 27th April 2012. Please come yourselves and invite others for as the Holy Prophet, may peace and blessings be upon him, said, 'Whomsoever guides towards a goodness, will get virtues equal to the one who performs that goodness.' [Muslim] You can get full directions from your place of origin to any destination around the UK by using the route planners on either Google Maps or Breakdown Cover | Car Insurance | Route Planner | RAC This lesson will be broadcast live on our website. For details on how to listen click here For further information: Call (+44) 116 246 1011 or visit Al Kawthar Academy::


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Contrasting habitat associations of imperilled endemic stream fishes from a global biodiversity hot spot
- Equal contributors
1 South African Institute for Aquatic Biodiversity, Private Bag 1015, Grahamstown, 6140, South Africa
2 Department of Ichthyology and Fisheries Science, Rhodes University, P.O Box 94, Grahamstown, 6140, South Africa
BMC Ecology 2012, 12:19 doi:10.1186/1472-6785-12-19Published: 26 September 2012
Knowledge of the factors that drive species distributions provides a fundamental baseline for several areas of research including biogeography, phylogeography and biodiversity conservation. Data from 148 minimally disturbed sites across a large drainage system in the Cape Floristic Region of South Africa were used to test the hypothesis that stream fishes have similar responses to environmental determinants of species distribution. Two complementary statistical approaches, boosted regression trees and hierarchical partitioning, were used to model the responses of four fish species to 11 environmental predictors, and to quantify the independent explanatory power of each predictor.
Elevation, slope, stream size, depth and water temperature were identified by both approaches as the most important causal factors for the spatial distribution of the fishes. However, the species showed marked differences in their responses to these environmental variables. Elevation and slope were of primary importance for the laterally compressed Sandelia spp. which had an upstream boundary below 430 m above sea level. The fusiform shaped Pseudobarbus 'Breede' was strongly influenced by stream width and water temperature. The small anguilliform shaped Galaxias 'nebula' was more sensitive to stream size and depth, and also penetrated into reaches at higher elevation than Sandelia spp. and Pseudobarbus 'Breede'.
The hypothesis that stream fishes have a common response to environmental descriptors is rejected. The contrasting habitat associations of stream fishes considered in this study could be a reflection of their morphological divergence which may allow them to exploit specific habitats that differ in their environmental stressors. Findings of this study encourage wider application of complementary methods in ecological studies, as they provide more confidence and deeper insights into the variables that should be managed to achieve desired conservation outcomes.


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Dripping Springs TX Real Estate & Homes For Sale
255 Properties Found
The median home value in Dripping Springs, TX is $304,600.
This is
higher than
the county median home value of $176,500.
The national median home value is $170,100.
The average price of homes sold in Dripping Springs, TX is $304,600.
Approximately 58.21% of Dripping Springs homes are owned,
compared to 29.29% rented, while
12.5% are vacant.
Dripping Springs real estate listings include condos, townhomes, and single family homes for sale.
Commercial properties are also available.
If you see a property you're interested in, contact a Dripping Springs real estate agent to arrange a tour today!
1 - 10 of 255 Properties Displayed
Courtesy: Keller Williams - Lake Travis
View additional info
Home built by Chris Elder Homes. Situated in the Texas Hill Country. 10ft ceilings, above and below cabinet lighting, granite countertops, raised upper cabinets, ss appliances, hand scraped wood flooring, natural chiseled edge Travertine. Home will be complete and ready for closing within 30 to 45 days.
Courtesy: CTI Real Estate Service, Inc
View additional info
This beautiful Hill Country home is located on a little less than 2 acres & many upgrades have been added by the owners since purchased. The home is located in the award winning DSISD, it is also located on a private street nestled in the back of West Cave Estates & on a cul-de-sac! Amazing location! The owners have taken great care of this property. This home is a must-see and will not last long. The two 18'x21' Sheds on the back of the property are negotiable! PROFESSIONAL PHOTOS COMING SOON!
Courtesy: Dripping Springs Realty
View additional info
This modern ranch home is perched high atop 5 beautiful hill country acres with terrific views & a creek across the back. For those who enjoy the great outdoors, you can meander through the native trees & plants, over the rocky outcroppings and hike the creek all the way down to the Pedernales River! This artist retreat has 3 full beds each with an en-suite bath along with a detached studio, several decks & a balcony to enjoy the distant views. Make this your weekend getaway or enjoy paradise full time!
Courtesy: Dripping Springs Realty
View additional info
Enjoy the stunning views from your heavenly hilltop home perched high on a knoll in a gated community in the beautiful Texas Hill Country. You're just minutes from Austin & the Bee Cave area for shopping & restaurants yet you get the feeling of living far from it all. This lovely Texas style home is replete with high-end finishes & walls of widows to bring the outside in. The equestrian lover will enjoy the huge lighted arena & 4 stall barn. Coveted Dripping Springs Schools & wildlife exemption in place!
Courtesy: Boyd & Boyd Properties
View additional info
Fabulous residence on top of the hill with exquisite views from almost every room. Private, spacious, and a rare find in the very desirable neighborhood of Sawyer Ranch. Great floor plan with stainless steel appliances, and kitchen open to the Family room. Wood-look tile in Family room. Master Bedroom downstairs, and Guest Bedroom, with private entrance and full bathroom also downstairs. 3 Bedrooms upstairs, with a HUGE playroom that leads into the very private Media room.
Courtesy: Coffman Real Estate
View additional info
Rare lot in Dripping Springs ISD that is zoned both single family or multi family. Several options for the person that wants to build a custom home, duplex for investment cash flow, or duplex for affordable housing if you live on one side and rent the other to offset mortgage payment. Plan and specs require HOA approval, see restrictions. The lot is flat with trees and plenty of building space back off the road for privacy.
Courtesy: Stanberry & Associates Inc.
View additional info
This is a remarkable 7.922 +/- Acre Gentlemens Ranch. This builder paid attention to all of the fine details in this gorgeous home. Lots of room with 3 large bedrooms and 2.5 bathrooms. A Beautiful covered patio is excellent for entertaining and outdoor cooking. The garage is separate with a workshop on one side and garage on the other with a covered dive through in between.Home has a 21K gallon Rain Water Collection system. This is the one that you want to call your dream home!
Courtesy: Keller Williams Realty
View additional info
Gorgeous custom home on quiet cul-de-sac on almost two heavily treed acres. Fantastic location near H.S. & in the heart of Dripping Springs, this home offers 3 bedrooms, 2 full and one half bath, study (with walk in closet), fml. dining, lg. kitchen w/breakfast open to family room w/ fireplace. Custom upgrades include wood a& overlay concrete flooring, granite in kitchen, baths, & laundry, stone fireplace, built in entertainment center in family room & built in bookcase in study & more.
Courtesy: Listing Spark
View additional info
Great showing property on nearly 2.5 acres of gorgeous hill country land! Enjoy country living in comfort! New features for 2015 include wood laminate flooring and carpet, paint, new roof and new HVAC! Owner financing available.
Courtesy: Rose Real Estate
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Fabulous, private home with larger tracts on 3 sides. Lots of windows. All closets are large. Home has screened porch on east side with small covered patio attached. Large front porch. Acreage is suitable for animals. Seasonal Creek at back. 24' X 40' detached garage/workshop with full bath, washer/dryer connections & extra sink - portion of garage could be converted into guest accommodations. Lovely outdoor sitting area under large oaks. Large, stone, antique cistern being used as swimming pool.
1 - 10 of 255 Properties Displayed
78620 Real Estate Listings Provided By:
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Have a question or need assistance? Please fill out the information below and we'll be glad to assist you.
Dripping Springs & Nearby CitiesDripping Springs Real Estate Driftwood Homes For Sale Henly Homes For Sale Lakeway Homes For Sale Point Venture Homes For Sale Wimberley Homes For Sale Bee Cave Homes For Sale Briarcliff Homes For Sale Manchaca Homes For Sale Mountain City Homes For Sale Buda Homes For Sale
Dripping Springs ZIPs78620 Homes For Sale


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Manager: Boxer Williams paralyzed after crashing motorcycle in Georgia
Boxer Paul Williams was paralyzed from the waist down Sunday after being involved in a motorcycle crash in the Atlanta suburbs and doctors said it is unlikely he will continue his career, his manager, George Peterson said Monday.
"From the waist down, he has absolutely no movement. He's in very good spirits, though," Peterson told The Associated Press. "He still believes he's going to fight again."
Williams severed his spinal cord after falling on his back and head when he was thrown from his motorcycle Sunday morning in Marietta, Ga., Peterson said. Williams has been listed in serious but stable condition on Monday at an undisclosed hospital.
Williams was scheduled to fight Saul Alvarez on Sept. 15 in Las Vegas but that event has been canceled, Peterson said.
Horse racing
Belle Royale defeated Quiet Oasis by a half-length and won the $250,000 Gamely Stakes at Betfair Hollywood Park in Inglewood, giving trainer Simon Callaghan and jockey Joel Rosario back-to-back wins in the Grade 1 race. In the $100,000 Los Angeles Handicap, Roman Threat led all the way to win by 1¼ lengths for Hall of Fame trainer Bob Baffert.
•Shackleford held off a furious rush to the finish by Caleb's Posse and won the $750,000 Metropolitan Handicap by a nose at Belmont Park in New York.
Evelyn Stevens won the Exergy Tour in Boise, Idaho, following a final-stage breakaway, firmly cementing her claim to a berth on the U.S. Olympic team for the London Games.
Stevens, of Boulder, Colo., took second place in a sprint with Germany's Claudia Haeusler after the 46.7-mile stage. But the 29-year-old Stevens finished far enough ahead of the pack to beat American Amber Neben for the overall title.
•Tim Duggan held off a chasing group of about a dozen riders to win the USA Cycling Professional Road Race Championship in Greenville, S.C.
Mamitu Daska took the lead a mile in, and she never looked back to win the 6.2-mile Bolder Boulder road race in Boulder, Colo. The 24-year-old Ethiopian covered the course in 33 minutes, 6.50 seconds. Janet Cherobon-Bawcom (33:23.57) was second, and American Deena Kastor (33:28.57) was third.
Kenya's Allan Kiprono was the men's winner in 29:54.43. Tadese Tola (30:03.73) was second and fellow Ethiopian Brihanu Gedefa (30:07.14) was third.
College baseball
Florida, ranked No. 1 in every major preseason poll, has been selected as the top seed for the 64-team NCAA Division I tournament. The Gators (42-18) will host one of 16 four-team, double-elimination regionals that begin Friday. The other national seeds, in order, are: UCLA, Florida State, Baylor, Oregon, North Carolina, LSU and two-time defending College World Series champion South Carolina.
NCAA finals
Steve Johnson of USC won his second consecutive NCAA tennis singles title with a 6-4, 6-4 victory over Kentucky's Eric Quigley in Athens, Ga. Stanford's Nicole Gibbs won the women's final, rallying to beat teammate Mallory Burdette 2-6, 7-6 (7-5), 6-3.
•Loyola of Maryland beat Maryland 9-3 to win the NCAA men's lacrosse title in Foxborough, Mass., behind Eric Lusby's four goals. Lusby set a record with 17 goals in the tournament.
The Mercury Insurance Open is seeking volunteers and ball kids for the 2012 event, which is set for July 14-22 at La Costa Resort and Spa. For more information, visit http://www.mercuryinsuranceopen.com/About/Volunteer.aspx or email Gail DeWeese at email@example.com. ... American Mardy Fish had a medical procedure last week to correct a heart problem, he told USA Today.
Compiled from U-T San Diego news services


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Nizza said Stellaris Hospitals have not asked Empire for reimbursement increases that are any greater than what they already receive from other insurers in the market. In order to maintain their position in providing high-quality health services, Stellaris Hospitals need an adequate revenue base. In the past six months, Stellaris Hospitals have reached renewal agreements with two national and two regional insurance companies. Empire has stood alone in its refusal to agree to reimbursement rates that are even close to those that other insurance companies in the market are paying.
As hospitals and physicians struggle, Empire Blue Cross Blue Shields' parent company, WellPoint, Inc., continues to report record revenues and profits. For 2009, WellPoint's total revenues were $61 billion with net income of $4.7 billion, nearly double its 2008 results. This is the same WellPoint that was recently criticized by a Congressional committee for raising rates by as much as 39% for individual insurance plans in California. The Congressional committee also rebuked WellPoint for raising rates for subscribers while spending millions on lavish corporate retreats.
"While the insurance conglomerates such as WellPoint raise insurance premiums and provide inadequate reimbursement to providers to maintain their bloated profits for their investors, Stellaris Hospitals have invested over $100 million in the last several years alone for new facilities and state-of-the-art technologies," Nizza said. "Our commitment is to our patients and the communities we serve, not to shareholders or Wall Street analysts. Insurers such as Empire need to recognize that sufficient reimbursement rates are necessary in order to sustain the fiscal well-being of
|Source: PRWeb
Copyright©2010 Vocus, Inc.
All rights reserved


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India celebrates 64th Republic Day
Defense Mnister AK Antony receives the chief guest King of Bhutan,... News PhotoBhutan,Celebration,Delhi,Guest,Horizontal,India,Jigme Khesar Namgyel Wangchuck,New Delhi,Parade,Politics,Receiving,Republic DayPhotographer Hindustan TimesCollection: Hindustan Times 2013 Hindustan TimesNEW DELHI, INDIA - JANUARY 26: Defense Mnister AK Antony receives the chief guest King of Bhutan, Jigme Khesar Namgyel Wangchuck during the 64th Republic Day parade celebration at Raj path on January 26, 2013 in New Delhi, India. India marked its Republic Day with celebrations held under heavy security, especially in New Delhi where large areas were sealed off for an annual parade of military hardware. (Photo By Sonu Mehta/ Hindustan Times via Getty Images)


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- Northern New York Newspapers
- Watertown
- Ogdensburg
- Massena-Potsdam
- Lowville
- Carthage
- Malone
- NNY Business
- NNY Living
- NNY Ads
Republican Matt Doheny is accusing his likely Democratic opponent in November, Rep. Bill Owens of Plattsburgh, of "selling out" his constituents in favor of big-moneyed interests.
Mr. Owens "can't quit (the) bill because he risks turning off SOPA spigot," the news release says.
The not-so-subtle suggestion from the Doheny campaign is that Mr. Owens's vote is for sale.
"These allegations are absurd," said Sean Magers, a spokesman for Mr. Owens. "Outrageous claims like these do nothing to promote a civil discussion on the issue of internet piracy."
This is in reaction to the Stop Online Piracy Act, which seeks to stem the tide of content theft on the Internet from foreign sites. Critics have called the legislation overly broad and harmful the Internet. Mr. Owens, a sponsor of the bill, has said he's open to changes in it; piracy is a problem that costs billions of dollars to the economy and hundreds of thousands of jobs, Mr. Owens has said.
The news release from Mr. Doheny lists industries whose members have donated to Mr. Owens, and calls that his "price" for "selling out his constituency."
But drawing a direct line from campaign contributions to support for a particular bill is a difficult and foggy proposition (I've learned the hard way). For example, "unions" and "lawyers" are two categories of SOPA supporters, and they've also been generous to Mr. Owens. Unions and lawyers, though, are big donors to Democratic causes, whether SOPA is on the table or not. Did the unions and lawyers that donated to Mr. Owens do it so that he would support SOPA? That's a huge stretch.
Mr. Owens is actually nearer to the top of the list of recipients of anti-SOPA cash than he is for pro-SOPA cash.
This war of words comes amid a pretty interesting discussion that I was having with Mr. Owens in the editorial boardroom today, when I asked him whether his calls for civility were consistent with some of his fundraising emails that label Mr. Doheny as "a far-right millionaire." More on that later.
Mr. Owens has received a whole lot of calls that oppose SOPA, but said in the editorial board meeting today that his support for the legislation remains.
"It's never too late to reconsider," Mr. Doheny said in a statement.
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Come meet Debbie Miller - Author of A King Salmon Journey. She will be at the Kiss and Release on May 16th at Blackie's Pasture. The book is simply wonderful.
On May 16th, with all the fan faire and celebration we could muster, 2000 Chinook Salmon Smoltz were released into Richardson Bay in Tiburon.
Thank you to all the entries we received to our poster contest. We received some amazing entries.
Check out our article in the SF Chronicle from March 17th, 2013!
San Francisco State University officials may allow a popular Tiburon salmon education and conservation program to remain at Romberg Center.
The Tiburon Salmon Institute won a 30-day eviction reprieve pending talks the university said are aimed at retaining the salmon project.
The university's about-face comes amid mounting political pressure that includes Lt. Gov. Gavin Newsom and state schools superintendent Tom Torlakson, who both serve as state university regents, state Sen. Mike McGuire, Rep. Jared Huffman and Marin officials.
We are shocked and dismayed that San Francisco State University SFSU sent a June 1st Demand letter ordering us to remove our project and equipment from it's home at the Romberg Tiburon Center for Environmental Studies RTC in Tiburon by July 1st. The project has been there for 42 years raising salmon and connecting with the community. The San Francisco Tyee Foundation and their partners established the project in 1973. The property is owned by San Francisco State University.
Our annual salmon display is underway with Salmon Signs painted by the community all over Mill Valley and Tiburon. We receive many compliments every year from them. Last Friday night someone took an exceptional amount of signs off Tiburon Blvd. If anyone has information regarding these signs and why someone took them, please let us know. If you know of a salmon sign that swam into someone's yard or property, ask them about making a $20,00 donation through pay pal on our web site. We enjoy putting them out, but they do take time to make and there is a cost to the supplies.
Come meet Debbie Miller - Author of A King Salmon Journey. She will be at the Kiss and Release on May 16th at Blackie's Pasture. The book is simply wonderful. It chronicles the journey of Chinook a King Salmon up the Yukon River and through Alaska. It is wonderfully illustrated and a beautiful story. It is a great compliment to the children's own release of a salmon at Blackie's Pasture. Your child will come away with an understanding of salmon and what they need to survive. Please come to the event and buy a copy of her book.
Do you need to fill community service hours for school? Come help us get ready for our Big Event!!!! We need help with stapling salmon hats, putting up posters all over Marin or soliciting donations to help cover the Big Kiss and Release. If you would like to help, please contact Brooke at email@example.com.
Hope to see you at Kiss and Release,
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text: Jordan Baker | IBDB: The official source for Broadway Information IBDB: The official source for Broadway Information Official Broadway League sites: - sites - broadway.org broadwayleague.com broadwayfanclub.com kidsnightonbroadway.com stagespecs.org tonyawards.com Quick Search For: In This Category: All Show People/Org. Theatre Season Jordan Baker Performer Female Relations: Wife of Kevin Kilner ProductionsAwards ProductionsDate of ProductionsThe Normal Heart[Play, Drama, Original] Understudy: Jordan Baker [Dr. Emma Brookner]Apr 27, 2011 - Jul 10, 2011Garden DistrictSuddenly Last Summer[Play, Original] Performer: Jordan Baker [Catharine Holly]Oct 10, 1995 - Nov 05, 1995 1996 Theatre World AwardGarden District [recipient]Performer: Jordan Baker Find out where Jordan Baker and are credited together © 2001-2015, The Broadway League, All Rights Reserved. (Copyright and Disclaimer)


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Showing off her amazing UK tan.
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I like em pale myself. She's a little chin-y, but she works. Also, bewbz.
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my subwoofer has more tone.
im digit the chick on the left!
Tits can make even the ugliest of women fuckable.
Wikipedia claims she is a multi-instrumentalist, yet all her songs seem to be computer generated. In any case, I prefer the brunette on the left.
3 gunts walk into a poolside bar …
It's nice to see that the Women's Studies class vacations together.


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Where are yall from? :)
Originally from Nebraska, we live in Houston, TX now and I love it.
Me {27} DH {31} .: 10/24/09 :.
{SAHM } to DD#1 Camilla Brielle 9/13/11
DD#2 Callista Brenna 1/13/13
Katy, TX (20 minutes outside Houston)
I keep seeing "x # of miles from such n such city. LOL, in Houston its about the minutes not the miles!!
FTM~ James Matthew made his debut May 11th 2010 weighing 8.2 lbs & 21 1/2 inches.
Skylar Payton due Sept 11th 2011 but arrived Sept 20th, 2011.
Our Angel came into this world Sept 13th... 6 years after my terrible car accident... <3
born in west virginia, moved to florida as a baby where i grew up. now im in southern california (orange county.. past 9 yrs)
Ashley & Esco 7.13.06
Austin Connor 6.11.03 and Sadie Elena 2.20.08
& Samantha Nicole 8.23.11


text:
This work represents an extensive MD simulation / water-dynamics studies on a series of complexes of inhibitors (leupeptin, E-64, E-64-C, ZPACK) and plant cysteine proteases (actinidin, caricain, chymopapain, calotropin DI) of papain family to understand the various interactions, water binding mode, factors influencing it and the structural basis of differential inhibition.
The tertiary structure of the enzyme-inhibitor complexes were built by visual interactive modeling and energy minimization followed by dynamic simulation of 120 ps in water environment. DASA study with and without the inhibitor revealed the potential subsite residues involved in inhibition. Though the interaction involving main chain atoms are similar, critical inspection of the complexes reveal significant differences in the side chain interactions in S2-P2 and S3-P3 pairs due to sequence differences in the equivalent positions of respective subsites leading to differential inhibition.
The key finding of the study is a conserved site of a water molecule near oxyanion hole of the enzyme active site, which is found in all the modeled complexes and in most crystal structures of papain family either native or complexed. Conserved water molecules at the ligand binding sites of these homologous proteins suggest the structural importance of the water, which changes the conventional definition of chemical geometry of inhibitor binding domain, its shape and complimentarity. The water mediated recognition of inhibitor to enzyme subsites (Pn...H2O....Sn) of leupeptin acetyl oxygen to caricain, chymopapain and calotropinDI is an additional information and offer valuable insight to potent inhibitor design.
Recently the cysteine proteases of both the plant and animals have received a considerable attention because of their broad ranges of activities and critical role in the different intracellular / biological / pathological processes or disorders [1-7]. Uncontrolled proteolysis of certain proteases e.g. Cathepsins [8,9], Caspases [10,11], Cruzain [12,13] causes several pathological disorders. Therefore the development of potential inhibitors which can modulate or can moderately control the proteolytic activity has become a challenge in drug design. For combating that challenge, some stimulating investigation on the structural aspects of the complexes on those kind of cysteine protease with different organic inhibitors or ligands e.g. E-64, E-64-C, leupeptin etc have been done by x-ray methods, which revealed the 3D-interactional information about the substrate binding chemistry and the topological requisite for substrate-mimicking inhibitors. However, due to the lacking of detail inhibitor binding information, we are interested to carry out those studies by MD-simulation methods on plant cysteine protease-inhibitor complex of papain [14,15] superfamily.
In this regard, beside the common Sn-Pn (n = 1–3) interactional events of the inhibitor with the main chain of the active site residues in the respective complexes, the role of the water molecules can not be ignored as they are sometimes present as conserved at the ligand binding sites of homologous proteins. These structurally conserved water molecule can change or influence the shape and complimentarity of the inhibitor site, thus affect strategies for therapeutic design. So, in order to get valuable insight of the characteristics of the inhibitor, the modeled inhibitor-enzyme complex structures are analyzed in detail and compared with the crystallographic information. The model structures of the enzyme-inhibitor complexes are built for the present study using template of available x-ray structure of enzyme-inhibitor complexes from papain family. In each complex structure, either from a x-ray or model study, it is evident that binding and catalysis is a two fold mechanism and in each case the subsite (Sn-Pn) interactions [16] are the main features for consideration to understand their differences in specificity.
The chemical structures of the selected inhibitors E-64, E-64-C, leupeptin, ZPACK for the present study are shown in Fig. 1. The inhibitor, E-64, a trans epoxysuccinic acid attached to a modified dipeptide [(leucylamino)-4-guanidinobutane] [17,18] is a potent irreversible (covalent type) inhibitor for cysteine proteases in general and its binding modes with papain [19], actinidin [20], caricain [21] and other lysozomal cysteine proteases [7] have been reviewed. The other analogue, E-64-C [22], consisting of epoxysuccinyl, leucyl, and an isoamyl group, is also being studied for its mode of different subsite binding [23-25]. The efficacy of this kind of inhibitor, makes the epoxide and its derivatives potential candidates as drug for the suppression of elevated levels of cysteine protease activity associated with certain disease states [23].
Figure 1. Chemical structures of Leupeptin, E-64-C, E-64 and ZPACK
Figure 2. Stereoscopic view of average over the last 50 ps of MD simulation of the Calotropin DI (backbone only) with E-64 (inhibitor, ball and stick) complex
Figure 3. Stereoscopic view of average over the last 50 ps of MD simulation of the Actinidin (backbone only) with E-64-C (inhibitor, ball and stick) complex
Figure 4. Stereoscopic view of average over the last 50 ps of MD simulation of the Caricain (backbone only) with Leupeptin (inhibitor, ball and stick) complex
Figure 5. Stereoscopic view of average over the last 50 ps of MD simulation of the Chymopapain (backbone only) with ZPACK (inhibitor, ball and stick) complex
Leupeptin is another biologically important peptidyl aldehyde (AC-leu-leu-Arginal), a naturally occurring protease inhibitor [26] of microbial origin. The arginal residue at the C-terminus of leupeptin is found to be essential for its inhibitory activity which inhibits cysteine proteases as well as serine proteases [27]. Aldehyde inhibitors such as leupeptin are promising lead compounds in the design of anticancer protease inhibitors [28,29]. They are mostly composed of amino acids and therefore have few metabolic degradation products that are toxic [27].
ZPACK (N-benzoyloxycarbonyl-L-phenylalanine-L-alanylchloromethyl ketone) [17,18], a substrate – like synthetic inhibitor [30], is specifically designed for cysteine proteases.
We have done model enzyme-inhibitor complexes for which no crystal structure is available with a view that, these structural study of the complexes by modeling along with the x-ray structures will afford further insight and complementary information in this area.
Results and discussion
All the inhibitors selected for the present study (E-64, E-64-C, Leupeptin, ZPACK) can form the covalent bond with active site Cys 25 Sγ atom (with distance ~1.80 Å) of each cysteine proteases. This crucial interactional step which is mainly manifested in their inhibitory activity seems to remain conserved in all of our considered thiol proteases (papain, caricain, chymopapain, actinidin, calotropin DI) belonging to papain superfamily. The study reveals that each of the inhibitor molecule forms hydrogen bonds with the S1 subsite residues of enzyme molecule almost in a same fashion (Table I) as were observed in x-ray structures. The main residues that are involved in the conserved interactions are Cys 25 Sγ, Cys 25 amide nitrogen, the side chain of Gln 19 Nε2H, His 159 Nδ1H and in some cases carbonyl oxygen of Asp 158. The other residue Gly 66 offers its carbonyl oxygen and amide nitrogen to form hydrogen bond with the backbone of inhibitor molecule in most of the cases which are shown in Table I. It is observed that all these interactions help to orient the inhibitor molecule suitably in the active site cleft for the required nucleophilic attack by the active Cys 25 Sγ atom in the complex structure [31]. The S1-P1interactions of each of the inhibitor with the proteases papain, actinidin, caricain, chymopapain and calotropin DI are included in Table I. Both the MD simulation study and solved enzyme-inhibitor crystal structure of papain superfamily reveal, although S1-P1 interaction remains conserved / similar in all cases, however essential differences arise mainly at the S2-P2 and S3-P3 side chain interaction sites, depending on the amino acid residues constituting the respective sites of the proteases. The reaction propensities of the enzyme residues of the model enzyme-inhibitor complexes can be looked from DASA results (Fig. 6 – Fig. 9) which show distinctly the residues involved in electrostatic and hydrophobic interaction with the E-64, E-64-C, Leupeptin, ZPACK. The interaction pattern obtained from MD-simulation study indicates that the differential interaction in the S2 subsite is a dominant factor in defining the substrate specificity of cysteine proteases of papain family. This S2 subsite, which is generally hydrophobic in nature, is the best defined substrate binding site that deserved the term pocket and involves the residues both from -R and -L domain of enzyme [31]. Table II shows the involved residues of the selected proteases in S2 subsite region. Another residue (205, papain numbering) Ser for papain, Met (211) for actinidin, Gln (205) for calotropin DI at the back of S2 pocket is found to play a significant role in deciding the subtrate specificity of these enzymes at the P2 position of an inhibitor. The residues involved in S3 binding site (Table II) are generally found to be present on the enzyme surface and the interaction seems to prevail only in the side chain region with no main chain interaction.
Figure 6. DASA study of inhibitor-enzyme complexes
Figure 7. DASA study of inhibitor-enzyme complexes
Figure 8. DASA study of inhibitor-enzyme complexes
Figure 9. DASA study of inhibitor-enzyme complexes
Table I. Significant atomic distances (Å) of different catalytic site residues of cysteine proteases with selected inhibitors at S1 - P1 site
Table II. Residues involved in S2 and S3 sites of different protease systems
Probable interaction of the inhibitors with the active site residues ofActinidin, Chymopapain, Caricain and Calotropin DI
Binding mode of E-64 and E-64-C to proteases (Caricain, Chymopapain, Actinidin, Calotropin DI)
All the main chain hydrogen bonding are similar to those found in papain complexes (Table I). The P1 carbonyl oxygens of E-64 and E-64-C forms hydrogen bonds with Cys-25 N, Sγ and Gln 19 Nε2H. The P2 main chain keto O and amide NH (Table III) make two hydrogen bonds with NH and O of Gly-66 respectively in most of the cases forming one residue stretch parallel β sheet structure. Although the interaction at the S1 subsite of enzymes are similar in nature for both of the inhibitors, significant differences arise at the P2 position while making interaction to S2 pocket of the enzymes. P2 residue (Leu) of E-64 extends inside into the pocket (Fig. 10) whereas corresponding P2 residue of E-64-C is located just at the entry of the S2 hydrophobic pocket (Fig. 11) interacting weakly. This point also gets support from DASA study (Fig. 6 – Fig. 9) for E-64-C-protease complexes where the peak for one of the leading residue at the S2 pocket is either very low or absent : Gly-130 (for calotropin DI), Ile-133 (for chymopapain), Val-133 (for caricain), Ala-136 (for actinidin). Another significant feature arising from MD simulation study that also get support from DASA study, is the residues of proteases especially Asp-158 in model complexes with E-64, E-64-C show a considerable decrease of their accessibilities suggesting that the Sn (n = 1–3) subsites of the modeled complexes are perfectly shielded from the solvent phase by binding with the inhibitors. The large difference in accessibility may occur due to many interaction with the inhibitor molecule. There is a second mode of binding for E-64-C to protease where isoamylamide and leu group of E-64-C involved in hydrophobic interaction with S2 and S3 site (contrary to S3 and S2 sites)of proteases respectively, but it does not change any basic interaction characters at these sites. In both cases the attack on the active site thiol group of the cysteine proteases occur from the backside of the inhibitor resulting in opening of the epoxide ring and at the same time the formation of a covalent bond between inhibitor and enzyme [19,20,32,33].
Figure 10. Stereoscopic view of probable binding mode of E-64 with the active site residues of Calotropin DI
Figure 11. Stereoscopic view of probable binding mode of E-64-C with the active site residues of Actinidin
Table III. Residues involved in various subsites of the selected inhibitors
Binding mode of ZPACK and Leupeptin to proteases (Caricain, Chymopapain, Actinidin, Calotropin DI)
Both ZPACK (N-benzoyloxycarbonyl-L-phenylalanyl choromethyl ketone) and leupeptin form hydrogen bonds with the proteases in model complexes in usual fashion (Table I). But the important feature of β sheet nature of hydrogen bonding pair formed between the P2 moiety of ZPACK (phe) and Leupeptin (Leu) (Table III) with protease Gly-66 is antiparallel, different from E-64/E-64-C-protease complexes. The bulky P2 sidechain of ZPACK, which is Phe, is comfortably accommodated in the space available making hydrophobic interaction with all the residues Leu-133, Leu-157 (for chymopapain), Ala-136, Ile-160, Met-211 (for actinidin), Val-133 and Val-157 (for caricain), Gly-130, Val-154, Gln-205 (for calotropin DI). The P2 moiety (Table III) extends itself far inside the S2 pocket and thus away from carbonyl oxygen atom of Asp 158 (Fig. 12) which makes P2 backbone unable to form hydrogen bond with this residue. DASA study done on the model complexes with ZPACK shows the peak for Asp-158 for respective enzymes notably less prominent compared to other complexes. Thus it can be argued that the absence of this particular H-bond makes this protease-inhibitor complex less stable compared to other protease-inhibitor complexes. The residues involved in S2 subsite interaction for ZPACK-protease model are also same and responsible for preference of substrate splitting in leupeptin-protease complexes (Fig. 13). P2 moiety of leupeptin and E-64, despite being inside the S2 pocket, orients its backbone in such a way that they are able to form the additional H-bond (Table I) with carbonyl oxygen atom of Asp-158 giving extra stability to the respective complexes. There is another pocket on the left of the cleft called S3 subsite defined by the segment 61, 67 (papain numbering). The CBZ (benzoyloxycarbonyl) group of ZPACK simulating its P3 residue in the present complex makes aromatic-aromatic interaction with phenyl ring of S3 subsite with the shortest distance from aromatic to aromatic group > 4Å (Fig. 12), whereas the P3 moiety (Leu) of Leupeptin extends its sidechain towards the surface of S3 site interacting with its constituent residues (Fig. 13) of proteases.
Figure 12. Stereoscopic view of probable binding mode of ZPACK with the active site residues of Chymopapain
Figure 13. Stereoscopic view of probable binding mode of Leupeptin with the active site residues of Caricain
Role of water in inhibitor binding to the respective proteases
Water molecules have endowed several important role in the recognition and stabilization of the interaction between the ligand and its site. Table IV(A,B,C) gives a generalized view of interaction with the water molecules in active site cleft of the proteases. Extensive MD simulation / water dynamics study done on a series of enzyme-inhibitor complexes from papain plant family demonstrates some unique features of interesting nature. Conformational and dynamic properties of the model complexes are compared among themselves and with respect to x-ray structure. Solvent networks found in the x-ray structure were reproduced by the simulation which was unbiased with respect to the crystalline hydration structure. These networks seem to play an important role in the stability of the complexes, evidence of this is found in the structure of the ligand water interaction of the active site. We have identified water molecules, by studying a series of MD simulated complexes, in the active site cleft that may be essential in binding of inhibitor in the cleft.
Table IV (A). Water involved in the interaction inside active site cleft of the enzyme-inhibitor complexes
Table IV (B). Water involved in the interaction inside active site cleft of the enzyme-inhibitor complexes
Table IV (C). Water involved in the interaction inside active site cleft of the enzyme-inhibitor complexes
In all Leupeptin-protease model complexes we found P1 side chain (Arg) of leupeptin is extensively involved in water interaction network (Table IV(A,B,C)). These water interactions though less in number, are also found to be present in x-ray structure of papain-leupeptin complex [34]. These interactions support the fact that P1 side chain of inhibitor, leupeptin, makes few interactions with S1 site to avoid steric hindrance with carbonyl group of Gly-23 of protease molecule and extend straight up out of the cleft towards solvent [8] making a number of interactions. Moreover the keto oxygen (O3) of ZPACK, epoxy and carbonyl oxygens of E-64, E-64-C seem to stabilize through strong and weak H-bond with water molecules within the groove of the enzymes. The amide nitrogen (N11) of ZPACK and keto oxygen (O9) of E-64, E-64-C are observed to form H-bonds with water sites thus assisting the chemical potentiality of P2 site. MD simulations also characterize some other important stereochemically potential interaction sites of inhibitors in complexation with different proteases associated with P3 site of E-64, leupeptin, ZPACK and involved in solvation network within the active site. Guanidinobutane group of Arg of E-64, keto oxygen (O13, O14) of CBZ of ZPACK and acetyl keto oxygen of leupeptin involved in water interaction at S3 site with each protease in model complexes (Table IV(A,B,C)). The water molecules easily access the surface region of S3 site of enzyme to interact with the polar group involved in P3 site of those inhibitor molecules. Solved x-ray structure of complexes from papain family also shows this solvation network of P3 site of inhibitor only for papain-E-64 [19] and actinidin-E-64 [20] complexes but few in number. The inhibitor bonded water molecular bridging H-bonds of leupeptin acetyl oxygen directly to protein S2 / S3 subsite residues at amide oxygen of Val-157 (in caricain) and Gly-66 (in chymopapain) and at side chain Tyr-67-OH group of calotropinDI are more conducive allowing the ligand binding by Pn...H2O...Sn type of interaction which are evidenced and are supported from our studies. Thus the hydration dynamic study on series of modeled complexes helps us to understand the role of water involved in stabilizing the inhibitor molecules in S2 / S3 subsite of proteases, where polar group involved in P3 site of inhibitor may add extra stability to enzyme-inhibitor complexes by water mediating interactions of ligand and enzyme residues involved in this segment.
The other key feature and the most important one that has emerged out from the study is the participation of a key water molecule (marked by ** in Table IV(A,B,C)) in the vicinity of oxyanion hole within the active site of proteases which seemingly play a vital role in inhibitor binding. This particular water molecule found near the region of oxyanion hole, comprising of Cys 25 NH and Gln 19 Nε2H, forms hydrogen bond with the carbonyl / carboxyl oxygen of P1 moiety of inhibitor at a distance of ~2.63 Å to 3.47 Å [O18 of E-64 and E-64-C, O3 of ZPACK and mainchainO of Arg of leupeptin] (Fig. 10 – Fig. 13). This observation when extended to the solved x-ray structure of papain family either native or complexed [15,19-21,33,35,36], we found that this particular water is also present in several of these cases (PDB codes are 1PPN, 2ACT, 2AEC, 1MEG, 1GEC, 1YAL, 1PPP, 1PE6). This important observation lead us to suggest that the water molecule present in the solved structure and model complexes may be involved in stabilizing the inhibitor molecule during nucleophilic attack of cysteine protease. And perhaps this water which behaves like a conserved water, is involved in acylation / deacylation step during catalysis by proteases in papain family. Table IV(A,B,C) highlights that for each inhibitor the interactions are not random, but rather highly specific, usually involving specific residues / regions of the ligand. These water molecules contribute directly to the stability of the complex by holding themselves in the right position through network of hydrogen bonds. These water networks are probably crucial for the stability of protein-ligand complexes and may be important for any site directed drug design strategies. Thus our observation demonstrates that incorporation of water molecules into the system improves interpretation and predictive ability of the models and are in good agreements with the x-ray structural analysis of some complexes and offers valuable insights into new characteristics of the ligands which may be exploited for design of more potent inhibitors.
Material and Method
The different inhibitors (Fig. 1) were docked to the active site cleft of the selected proteases from the papain family using chemical intuition and viable interaction strategy. The position and orientation of these inhibitors in the active site cleft was primarily based on the knowledge of x-ray structures [19-21,30-34,37-39] of different cysteine proteases with the inhibitors.
Each of the modeled structures was subjected to constrained energy minimization to relieve residual strain maintaining the integrity of the model. The modeling and energy minimization was done with the InsightII software package (Molecular Simulations Inc., San Diego, CA, version 95.0) on a Silicon Graphics Indigo workstation. The minimization was done with the Discover Platform of the InsightII program with a consistent valence force field (CVFF). The algorithms utilized for minimization were typically the conjugate gradient options in vacuum and were chosen to be distance dependent. The covalently linked ligand was minimized while the enzyme backbone was kept rigid using the Discover option constraint. During energy minimization, in addition to the usual energy terms (bond, angle, dihedral, improper dihedral, electrostatic and van der Waals), distance constraints were also introduced initially. Different selected inhibitors were docked into the caricain [21,40], chymopapain [36], actinidin [35], calotropinDI [41] environments lead by the information of x-ray crystallographically solved complex structures of proteases from the papain family. The nonbonded interactions included van der Waal's and electrostatic forms which used the Lenard-Jones and Coulomb functions respectively. The cut off radius for nonbonded interactions was 30 Å.
Molecular dynamics (MD) simulations of the modeled enzyme-inhibitor complex structure
Each of the energy minimized structure was solvated by a 10 Å layer of water molecules using SOAK option of insight II (Molecular Simulations Inc., San Diego, CA, version 95.0). During molecular dynamic simulations and minimization, a distance dependent dielectric constant of 1.0 was used. The parameter used was the consistent valence forcefield (CVFF). Prior to simulations, the co-ordinates of all the enzyme-inhibitor atoms were fixed and solvent molecules were minimized using steepest decent method in all constrained minimizations for 1000 iterations. Finally, the conjugate gradient minimizations were performed with all atoms free to move until maximum derivative was < 0.5 Kcal/mol/Å. The minimized co-ordinates of the whole system were used as a starting point for NVT (constant volume and temperature) at 300K to generate possible stable conformation. After 20 ps equilibrium stage, the simulations were carried out using velocity verlet algorithm for another 100 ps. Only the amino acid residues and solvent within a 12 Å radius of the inhibitor was permitted to move during simulation; the remainder of protein was fixed throughout the simulation. Surrounding the sphere of moving residue and solvent was a layer of waters whose positions were fixed in order to ensure that none of the moving solvent molecules escaped from the vicinity of the active site simulation. The average structures over the last 50 ps of each kind of MD simulations were taken to find out probable interactions of the inhibitors with the active site residues (Fig 2 – Fig. 5).
All the optimized structures were finally analyzed by PROCHECK [42].
Solvent accessibility study
The difference accessible surface area (DASA) study of a protein with and without inhibitor reveals the potential interactional sites of the protein. In order to estimate the fit of contact between the protein and the inhibitor, the difference accessible surface area (DASA) was calculated using DASA = [ASA (enzyme) – ASA (complex)] where ASA represents the accessible surface area given by Lee and Richard [43] (1971). ASA (complex) and ASA (enzyme) are the accessible surface area of each residue in a protease structure with and without the inhibitor respectively. The DASA study, performed on each of the modeled complexes using Biosym (MSI, San Diego, CA, InsightII package, 95.0 version), shows distinctly the protease residues involved in electrostatic and hydrophobic interaction with the inhibitors. Difference plots of solvent accessibilities to indicate the interacting residues of different numbers of papain-family cysteine proteases are shown in Fig. (6,7,8,9).
We are thankful to the members of the Biophysics Department, Bose Institute. Prof. N. K. Sinha for his assistance and Prof. W. Saenger for providing us with Calotropin DI co-ordinate at 1.9 Å in 1992 through personal communication.
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text:
What's the name of this song? can any1 tell me?
Thanks for helping us associate the correct Pornstars to this video!
Help Us: Do these Pornstars appear in this video?
Pornstar Name:
All Comments (94)
What's the name of this song? can any1 tell me?
i want know the name of this song too !!
Its Pillar - Fireproof.
What a beautiful girl !!!!
u guy really ask for the song? isnt that a sad youtube thing?
insane body!!
Nice Chick Whoow xD
Sweet song with an even sweeter girl combined! Excellent! xD
excellent pussy.... boobs fentastic.....i like this girl...
The nice one of youporn
does anyone konow who that chic is?
The name of this chick is nikki
sick song + nice strip = win!
yeah thats a perfect girl
That's all I have to say! lmfao
fittest girl on youporn. easily.
shes one of the many girls that are actually too pretty for doing porn
some good shit that
Please tell me this girl has more movies.
Her NAME is Naomi McKidney
man i cant find this girl anywhere else but here dose she have a web page
ähh yes ANITA SCH...
I know that girl it goes in the class 8 a it is ANITA DAM!!!
i was on the other side of the caera on my pc. holy shit!
This is a very beatifuk girl shes naturaly sexy ! I also wann know whats the name of this song / Group ?
My 2 favorite things in the same video hot girls and good music darn that girl is hot
La ragazza piu' bella del mondo...!
wow, really are perfect tits!
Most beautiful girl I have ever seen hands down
hottest chick ive seen on here
She is a perfect lady !!
hey this gurl needs to chill with me i am looking for a hot girl like her
WOWEEEE! Her parents created a perfect body. When will they make better cam cameras?
A girl cannot be more perfect. You must see and enjoy what you see.
perfect pussy so sexy
i wanna fuck whit her
Perfect girl. Inviting, her pussy has to be handled by a fit man.
jesus... just one thing to say... PERFECT!
damn hot chick
I think i'm in love guys xD
i like her pussy, its not all fucked up
Gotosaaaaa. Hot hot hot. Brasil love you
shes perfect
I want to lick these pussy
i want to eat her pussy mmmmmmmm
... well I plan to spank my monkey for 4 o 5 videos ... as always... and this is first video I see... surprise! no more videos today... lol
One of my 2 favorite girls... God she's human perfection. Cute face, amazing body, beyond perfect breast and a pussy that literally begs for cock. 11/10.
The song playing is "Fireproof," a song by the Christian rock group Pillar (whose frontman is involved in some anti-porn activism). I'm just really amused by this whole scenario.
oh baby !!
This chick has too many videos of her sitting in her chair and just stripping. At least fucking play with yourself and get off, or bring in another chick to play with, or even a dude to pound you. SOMETHING!
omg omg omg omg O M F G!! What a face, what titts, what a body and what a tasty pussy .. too hot for porn!
face... ridiciously beautiful.... tits perfect size/shape... pussy: would marry her for that masterpiece... and last but most certainly not least that butt....
You're so beautiful ... looks like the actress that Chuck is that blonde = D
she is hot she should be a star im stuck masturbating on this ONE video
THUMBS UP IFU THINK THIS IS SEXY! ohh wait...
@"WTF" Shut Up!
damn, after years she's still my favorite on youporn
reminds me of me
Perfect body and boobs
anyone know this girls name?
She has the most perfect pussy ever!
She ist so perfect. I've never seen such a perfect body.
Song is Shine by Collective Soul. The chick is hot and she likes good music!!
The song is called fireproof by a christian band called pillar. lol look it up.
i like her great body good music
Naomi McKidney doesn't seem to be her name. I checked best I could.
The Name is Nikki, but it's hard to find more videos of her. Theres one where shes lying sideways and a lot where she strips on a chair... but that's it
looks like nina terror
i want to have sex with you..i really like this...
It's really sad that beautiful girls like her waste their lives as porn stars, really...
She's totally fucking hot
More Bogus Music to ruin the view...
By far the hottest girl I've ever seen
CHICA PERFECTA, FELICIDADESLeave a comment...
her name is nikki Dimaggio
What a honey. I could wank for her three times a day and she gets me spurting every time. :)
what's with the shit music that gets put on these vids. WHY
whats the real name of the girl?
Who is this girl? I wanna see so much more of her!!!
I want to fuck you baby ah ah ah
These girls are too hot and nice boobs man I like this
She may be a beginner but she does it like a professinal


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Rosemary Breakfast Potatoes. Buddha's Delight - Recipe. Traveling in Italy: Aubergine Rolls with Ricotta & Speck. The strong smell of tomatoes mixed with aubergines, basil & ricotta was wafting throughout the house.
"The house smells incredibly good," my husband greeted me as he entered the house. I knew he would say that because the combination of ingredients of the aubergine rolls is his favorite. This was his dinner, while the kids and I would be having fish with aubergine salad. Like me, both kids have a particular liking of aged cheese. Fresh cheese never attracted me but I am giving myself a chance to try out this dish. Amongst my big bundle of gastronomic souvenir from our vacation in the Dolomites, are some speck that should perk up the taste. I have to admit, the smell compelled me to grab myself a couple of rolls.
Carnal Dish. Carnal Dish My name is Resha and I love to cook!
I have many recipes in the pipeline, so sit tight ;) Food you crave, food you lust....food that'll get you laid ;) Carnal: [kahr-nl] –adjective 1. pertaining to or characterized by the flesh or the body, its passions and appetites; sensual: carnal pleasures. Dish: [dish] –noun 3. the food served or contained in a dish: The meal consisted of several dishes. **all photos taken by me. please ask for permission to use them other than reblogging. Gajar Matar (Carrots With Green Peas) ~ The Vegetarian Recipes. Gajar Matar is a healthy and easy recipe to make.
This mildly spiced, colorful dish can compliment just about any meal. Recipe will serve 4. Ingredients 2 cups carrots diced in about 1/2 inch pieces 1 cup potato cubed in about ½ inch pieces 1 cup green peas 2 tablespoons oil 1 teaspoon carom seeds (ajwain) 4 whole red chilies 1 tablespoon coriander powder (dhania) 1/2 teaspoon turmeric (haldi) 1 teaspoon salt 1 teaspoon lemon juice How to make?
Butternut, Roasted red pepper and Halloumi Salad. Winter Salad – Grilled Butternut, Roasted Red Pepper & Halloumi spicy butternut, roasted red pepper & halloumi salad In winter, I miss the bounty of fruit and vegetables available in abundance in the spring and summer months.
I miss crispy salads served with refrigerator-cold ingredients, the colder the better. Leaves alone are even more unappetising in winter than they are in summer, to me. Here, I offer you a winter wonderland salad, filled with the brightest colours and warming ingredients that are as healthy for the body as they are cheering for the spirit. The quantities you use will be determined by how many servings you wish to make (no surprises). Ingredients Serves 2 2 handfuls butternut cut into finger-sized batons, coated lightly in cumin & chilli flakes 3 T grilled red pepper slices 5 slices (thin) halloumi 2 cups salad leaves and herbs (of your choice) salt, to taste olive oil & balsamic vinegar Method On a plate arrange the salad leaves and herbs.
Toss and serve immediately. You don't have to be fearful of vegan cooking. April 07, 2014 Police investigating two shooting incidents by Jim Redden Police are investigating two shooting incidents with no reported victims.
According to police, at 7:28 a.m. on Sunday, April 6, North Precinct officers responded to multiple reports of gunshots… Pioneer Square celebrates 30th birthday by KOIN News 6 Portland's Pioneer Square hit a major milestone on Sunday. April 06, 2014. Jamie's 30-Minute Meals recipes - Channel4 - 4Food. The Fare Necessities: Roasted Red Pepper Sauce with Pasta. Creative name, huh?
Look, while everyone else is starting to cook lighter meals because of the nicer weather, I'm thinking of how crappy my week was. Nothing particularly went wrong, it's just that, I'm tired. From school, from life, from having water stuck in my ear (which incidentally, makes me feel like when I talk, I'm screaming, because I can't hear right, so I talk louder). Regardless, I'm looking more for a comforting meal, rather than something light. Enter: Roasted Red Pepper Sauce. Ingredients: 2 red peppers (or one full jar, drained)¼ of an onion1 tablespoon olive oil¾ cup heavy whipping cream2 tablespoons butter (or margarine)½ pound of short cut pasta1 teaspoon salt½ teaspoon pepper½ teaspoon garlic powderFreshly grated Parmesan cheese, for topping Cut onion in half, and then in half again. Place onion and peppers on a foil lined cookie sheet. Drizzle olive oil on onion. Place under broiler. Place peppers in a bowl and cover. Put onions in food processor and set aside.
The Delicious Life. Maple ♥ Spice. 100 Recipes for a Healthy Summer. Summer is finally here!
It's time for BBQ's and outdoor dinner parties with friends and family. We want to make it easy for you to make healthy dishes for your family, but still enjoy the cooking activities that define summer. We collected a list of 100 healthy recipes for you to enjoy all summer long! Appetizers 1. 2.


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Lens+ is suprisingly good at what it does.
Lens+ is an easy to use retro camera app with a bunch of filters that look great. I'd say it's not a bad app at it's normal price of $1.99. Right now, it's been discounted for a very limited time to FREE. Read More


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The Dorset Heathlands Planning Framework SPD has been updated to cover the period 2015-2020 to take account of changes in the planning system, most notably the introduction of the Community Infrastructure Levy and its relationship with s106 agreements. In addition, the arrangements that have been in place since the introduction of the Interim Planning Framework in 2007 have been reviewed and the draft document reflects the new arrangements agreed by the local authorities.
The original SPD was adopted by the Borough of Poole at its Full Council meeting of 26th June 2012. The SPD came into effect on the 20th September 2012. Subsequently the Council at its meeting on 22nd April 2014 extended the timeframe of the document until 31st December 2014.
The SPD replaced the Dorset Heathland Interim Planning Framework (IPF). In practice there was no change to the way that the SPD works compared to the previous IPF. However, the advantage and purpose of converting the IPF to a SPD was to bring it inline with the current planning system with it becoming part of each of the participating local authorities Local Development Plans.
Consultation on the draft updated SPD commenced on Wednesday 7th January and closed on Wednesday 18th February 2015. The public consultation document can be found at the top of the downloads column to the right of this page. A hard copy can be obtained from the Council offices and local libraries of the relevant local authorities i.e. Bournemouth, Christchurch, Dorset, East Dorset, Poole and Purbeck.
The timetable for the updated SPD is set out below:
Consultation Document - 7th January to 18th February 2015.
Consultation Statement - Issues raised and Officer responses.
Adoption Statement
Adopted - Summer 2015.
The consultation on the draft Dorset Heathland Planning Framework SPD 2015-2020 has now closed. Representations and changes that are necessary are being considered. It is likely that a revised version will be available during the summer of 2015.
One aspect of the draft SPD is being implemented ahead of the document as a whole by the Borough of Poole, and this relates to the introduction of a charge for Strategic Access Management and Monitoring. Residential development that cannot avoid or mitigate its own adverse effects upon the Dorset Heaths Natura 2000 sites is required to make a contribution towards the overall Avoidance and Mitigation Strategy. This strategy has two elements, Heathlands Infrastructure Projects which in Poole's case are funded through the Community Infrastructure Levy (CIL), and SAMM, which is non infrastructure provision such as wardening, education and monitoring.
SAMM is not infrastructure and cannot be funded through CIL and therefore as from 8th April 2015 a new charge will be payable to cover this element of the strategy. The introduction of this charge was approved at a meeting of the Council's Cabinet on 24th March 2015 and will ensure that residential development is able to continue to be granted planning permission subject to other considerations. The SAMM charge is also effective for prior approval applications for the change of use and or conversion of non residential development to housing. This means that any new dwelling that comes forward by whatever course will pay a direct contribution toward mitigating the adverse effects of its impact upon the Dorset Heaths.
The charge is based on the cost of delivering SAMM in Poole and will be:
For a house £355
For a flat £242
The contribution is payable through either an Unilateral Undertaking or Section 111 agreement, together with an administration fee of £50 or 2%, whichever is greater. Standard templates for these can be found on the Section 106 Planning Obligations page under the downloads section, which is in the planning area of the website or by clicking here.
Supplementary Planning Documents
Local Development Framework
Email email@example.com
Telephone 01202 633321
Address Planning & Regeneration inc Building ConsultancyCivic CentrePooleBH15 2RU