text:
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JOURNAL OF CLINICAL MICROBIOLOGY, May 1996, p. 1313–1316
Copyright ? 1996, American Society for Microbiology
Vol. 34, No. 5
Detection of Parvovirus B19 DNA in Bone Marrow Cells by
Chemiluminescence In Situ Hybridization
Institute of Microbiology1and Department of Pharmaceutical Sciences,2
University of Bologna, Bologna, Italy
Received 30 October 1995/Returned for modification 16 January 1996/Accepted 15 February 1996
A chemiluminescence in situ hybridization method was developed for the search of B19 parvovirus DNA in
bone marrow cells, employing digoxigenin-labeled B19 DNA probes, immunoenzymatically detected with a
highly sensitive 1,2-dioxetane phosphate as chemiluminescent substrate. The light emitted from the in situ-
hybridized probe was analyzed and measured by a high-performance luminograph connected to an optical
microscope and to a personal computer for the quantification of the photon fluxes from the single cells and for
image analysis. The chemiluminescence in situ hybridization was applied to bone marrow cell smears of
patients with aplastic crisis or hypoplastic anemia, who had been previously tested by in situ hybridization with
colorimetric detection, dot blot hybridization, and nested PCR. The chemiluminescent assay provided an
objective estimation of the data, proved specific, and showed an increased sensitivity in detecting B19 DNA
compared with in situ hybridization with colorimetric detection.
Human parvovirus B19 is the etiologic agent of a wide range
of clinical syndromes such as erythema infectiosum, fetal hy-
drops, postinfectious arthropaty, transient aplastic crises in
patients with haemolytic disorders, and chronic bone marrow
failure in immunocompromised patients (1, 4, 26). As parvo-
virus B19 cannot be efficiently grown in established cell cul-
tures, the diagnosis of B19 infection relies mainly on the de-
tection of specific immune response to B19 and/or detection of
B19 genomes by hybridization assays or by PCR (11, 18, 20, 29,
31). In situ hybridization is a successful method for the local-
ization of specific viral nucleic acids inside individual cells, with
the preservation of cellular morphology also permitting retro-
spective studies on archival specimens. In situ hybridization
techniques have been successfully used for the detection of
B19 nucleic acids in bone marrow cells and in fetal tissues
using either isotopic or nonisotopic probes such as biotinylated
or digoxigenin-labeled probes (11, 17, 25, 27, 28). Digoxigenin-
labeled probes have proved very sensitive and specific for the
detection of B19 and other virus genomes in cells or tissue
specimens, giving levels of positive signal similar to those ob-
tained with radioactive probes and proving more sensitive and
specific than biotinylated probes (9, 19, 24).
In in situ hybridization assay, digoxigenin-labeled probes are
immunoenzymatically revealed by using antidigoxigenin Fab
fragments labeled with alkaline phosphatase or with peroxi-
dase followed by colorimetric substrates giving a final colored
product at the site of the enzyme (11). In recent years, chemi-
luminescent substrates have been proposed as a more sensitive
alternative to colorimetric substrates in various analytical tech-
niques in which small amounts of analytes or enzymes have to
be detected (7, 14–16, 22), and they have also been successfully
employed for the search of B19 DNA in dot blot hybridization
assays (13, 23, 30). Moreover, using newly synthesized dioxet-
ane derivates as chemiluminescent substrates for alkaline
phosphatase, we were able to detect as little as 10 fg of B19
DNA in a dot blot hybridization format, with the chemilumi-
nescent substrates proving about 10- to 50-fold more sensitive
than the colorimetric ones (12). Continuing improvements in
chemiluminescent substrates have been matched by new de-
velopments in photon imaging instrumentation such as high-
performance luminographs based on a charge-coupled device
video camera or high-dynamic-range pickup tube (Saticon)
combined with a video amplifier. These instruments not only
allow a quantification of emitted light at the single-photon
level but also permit localization of the chemiluminescent
emission on a target surface. Moreover, by connecting the
luminograph to an optical microscope, it is possible to localize
the light emission inside tissues or cells. All these data
prompted us to explore the use of highly sensitive chemilumi-
nescent substrates and a high-performance luminograph con-
nected to a light microscope for the in situ detection of B19
DNA inside bone marrow cells of patients with aplastic crisis
or hypoplastic anemias using digoxigenin-labeled B19 DNA
probes constructed in our laboratory.
Samples. Bone marrow aspirates from 15 patients with
aplastic crisis or hypoplastic anemias caused by B19, diagnosed
by in situ hybridization with colorimetric detection and dot blot
hybridization or nested PCR in peripheral blood serum sam-
ples were analyzed in the study (2, 11, 20, 21, 31). Dot blot
hybridization was performed with digoxigenin-labeled probes
as previously described (2), and the sensitivity of the assay with
colorimetric detection ranged between 1.5 ? 105and 3 ? 104
genome copies. Nested PCR was performed as previously de-
scribed (20) on peripheral blood samples, treated with a lysis
solution, and the sensitivity of the assay was between 1 and 20
Of the 15 patients, 6 were bone marrow transplant recipi-
ents, 6 were patients with lymphoproliferative disorders under
immunosuppressive therapy, 2 were thalassemic patients, and 1
was an AIDS patient with haemophilia. Moreover, samples
from a bone marrow recipient with hypoplastic anemia who
had proved positive for B19 DNA by in situ hybridization but
negative by dot blot hybridization and nested PCR in periph-
eral blood were also analyzed.
As controls, bone marrow aspirates from 24 patients with
* Corresponding author. Mailing address: Institute of Microbiology,
Via Massarenti 9, 40138 Bologna, Italy. Phone: 39 51 302435. Fax: 39
51 341632.
Page 2
hematologic disorders who had proved negative for B19 DNA
by in situ hybridization with colorimetric detection, dot blot
hybridization, and nested PCR in peripheral blood were stud-
Bone marrow aspirates from each patient were processed for
chemiluminescent and colorimetric in situ hybridization with
the same probes and in the same run. Samples were analyzed
in duplicate for both chemiluminescence detection and color-
imetric detection.
As negative controls cells from the interleukin 3-dependent
leukemic cell line TF-1 were used.
B19 parvovirus DNA probe. The B19 probe was prepared
from the molecular clone of a 5.0-kbp insert (nucleotides 282
to 5310), which represents the complete coding sequence of
B19 DNA, cloned in vector pUC18 (10). Routine methods for
large-scale preparation of plasmids were used. Digoxigenin-
labeling of the probe was performed as previously described
(2) from the excised insert, by the randomly primed DNA
labeling method. The digoxigenin-labeled probe was hybrid-
ized with serially diluted unlabeled parvovirus B19 DNA
bound to nylon membranes, and between 0.5 and 0.1 pg of B19
DNA (corresponding approximately to 1.5 ? 105and 3 ? 104
genome copies) could be visualized by colorimetric detection,
and 10 fg (corresponding approximately to 3 ? 103genome
copies) could be visualized by chemiluminescence detection.
The B19 DNA probe could be stored at ?20?C for at least 1
year with no decrease in its activity.
Bone marrow smear preparation. Bone marrow aspirates
were smeared on slides pretreated as previously described (11)
and were air dried and fixed with 4% paraformaldehyde in
phosphate-buffered saline (PBS) (0.15 M, pH 7.4) for 10 min.
After fixation, smears were washed three times in PBS for 10
min each and then dehydrated with ethanol washes (30, 60, 80,
95, and 100%) for 5 min each. Smears were then air dried and
stored at 4?C until use.
In situ hybridization reaction. Cell smears were hydrated in
PBS and placed in 0.02 N HCl for 10 min. After three washes
with PBS, cells were treated with 0.01% Triton X-100 in PBS
for 2 min. After three further washes with PBS, cell smears
were treated with pronase (Boehringer, Mannheim, Germany)
(0.5 mg/ml in 0.05 M Tris-HCl [pH 7.6], 5 mM EDTA) for 5
min. Smear preparations were then washed twice with PBS
containing 2 mg of glycine per ml. After these treatments cell
smears were postfixed with 4% paraformaldehyde in PBS and
washed twice with PBS containing 2 mg of glycine per ml.
Smears were then dehydrated by ethanol washes (30, 60, 80, 95,
and 100%). Dehydrated monolayers were overlaid with 10 ?l
of the hybridization mixture. The hybridization mixture con-
sisted of 50% deionized formamide, 10% dextran sulfate, 250
ng of carrier calf thymus DNA per ?l, and 2 ng of digoxigenin-
labeled probe DNA per ?l in 2? SSC buffer (0.3 M NaCl, 0.03
M sodium citrate [pH 7.0]). A clean glass coverslip was then
applied, and the edges were sealed with rubber cement to
prevent loss of the mixture during denaturation and hybridiza-
tion. Cell smears and hybridization mixture were denatured
together by heating in an 85?C water bath for 5 min and were
then incubated at 37?C overnight. After hybridization, the cov-
erslips were carefully removed, and cell smears were washed in
stringent conditions (11).
Chemiluminescence detection. For the detection of hybrid-
ized probes, slides were briefly washed in a 100 mM Tris-HCl
buffer, pH 7.5, containing 150 mM NaCl. Smears were then
incubated for 30 min with sheep polyclonal antidigoxigenin
Fab fragments, conjugated to alkaline phosphatase, and di-
luted 1/500 in blocking reagent (Boehringer). After incubation,
bone marrow smears were washed by two 15-min washes with
Tris-HCl buffer and equilibrated for 2 min with equilibration
buffer (100 mM Tris-HCl, 100 mM NaCl, 50 mM MgCl2[pH
9.5]). The chemiluminescence detection of alkaline phos-
phatase was performed by treating the cells with undiluted
adamantil-1,2-dioxetane phenyl phosphate substrate (CDP-
star) (Tropix, Inc., Bedford, Mass.). After an optimized incu-
bation of 30 min at room temperature, the solution was re-
moved, and the luminescent signal from the hybrid formation
was detected and analyzed with a system which consisted of
luminograph LB-980 (EG&G Berthold, Bad Wilbad, Germa-
ny), which is a high-performance, low-light-level imaging ap-
paratus with a high-dynamic-range pickup tube (Saticon) com-
bined with a video amplifier, connected to a model BH-2 light
microscope (Olympus Optical, Tokyo, Japan) and to a per-
sonal computer with a commercially available program for
image analysis. The microscope was enclosed in a dark box to
prevent contact with the external light. The system operated in
consecutive steps. Firstly, bone marrow cells were recorded in
transmitted light; then, the luminescent signal from the hybrid
formation was measured; and then, after a computer elabora-
tion of the luminescent signal with pseudocolors corresponding
to light intensity, an overlay of the two images on the screen
provided by the transmitted light and by the luminescent signal
allowed the spatial distribution of the target analyte to be
localized and evaluated. Digital images of the light emission
from bone marrow cells were optimized with 2-s integration
intervals for 1 min of total accumulation time. The light emis-
sion from each cell was quantified by defining a fixed area and
summing the total number of photon fluxes per second from
within this area.
As negative controls TF-1 cells were analyzed as described
above, providing threshold background levels. A mean of 50
negative cells were analyzed for each run, and the average
value of photon fluxes per second plus fivefold the standard
deviation was considered the positive threshold for chemilu-
minescence in situ detection of B19 DNA. Corrections for
instrumental background and flat-field variations were auto-
matically performed by the LB-980 apparatus.
To assess the specificity of the chemiluminescence assay, a
series of controls were performed: (i) B19-positive bone mar-
row cells were hybridized with the unlabeled B19 DNA probe;
(ii) B19-positive bone marrow cells were hybridized with the
unlabeled B19 DNA probe; (iii) B19-positive bone marrow
cells were hybridized with the digoxigenin-labeled B19 DNA
probe, but the incubation with anti-digoxigenin antibody was
replaced with an incubation with nonimmune sheep serum;
and (iv) B19-positive bone marrow cells were hybridized with
the digoxigenin-labeled B19 DNA probe, but the incubation
with antidigoxigenin antibody was omitted. The light emission
from control experiments was analyzed and evaluated as de-
scribed above.
Colorimetric detection. Hybridized bone marrow cells were
treated with the colorimetric alkaline phosphatase substrate as
previously described (11), and the development of a dark blue
precipitate at the enzyme site in positive cells was monitored
by microscopic examination.
Of the 15 bone marrow aspirates from patients who had
been proved positive for B19 DNA both by in situ hybridiza-
tion and by dot blot hybridization and/or nested PCR, all 15
samples proved positive by chemiluminescence in situ hybrid-
ization (Fig. 1). When a comparison between the numbers of
cells found positive by chemiluminescence in situ hybridization
and by colorimetric in situ hybridization was made, after the
samples from the same patient had been processed in the same
run and with the same batch of probe, the two methods showed
a high correlation (P ? 0.0005; Spearman rank correlation).
Page 3
With the chemiluminescence method a mean of 19.8 positive
cells per 100 counted cells could be found versus the mean of
4.6 positive cells per 100 counted cells obtained with the col-
orimetric method. This difference was highly significant (P ?
0.0007; Wilcoxon signed test for paired data).
The bone marrow samples from the patient with hypoplastic
anemia who had been proved positive by in situ hybridization
with colorimetric detection but negative by dot blot hybridiza-
tion and nested PCR in peripheral blood was confirmed pos-
itive by chemiluminescence in situ hybridization.
Of the 24 bone marrow aspirates from patients who had
previously proved negative for B19 DNA by in situ hybridiza-
tion with colorimetric detection, by dot blot hybridization, and
by nested PCR in peripheral blood, 23 samples proved nega-
tive by both chemiluminescence in situ hybridization and col-
orimetric in situ hybridization. One sample, however, proved
positive by chemiluminescence in situ hybridization but nega-
tive by colorimetric in situ hybridization. The sample was from
a patient with a chronic anemia after bone marrow transplan-
tation. To establish whether this sample was a false positive, we
tested the sample with another B19 DNA probe of 700 bp that
we had used in previous studies (2, 11, 23, 31), and the positive
result obtained by the chemiluminescence in situ hybridization
and the negative result obtained by the colorimetric reaction
were confirmed. As the peripheral blood from this patient
when retested with nested PCR was confirmed negative for
B19, the nested PCR was performed directly on bone marrow
cells, and a clear positive result was achieved.
A series of control experiments definitely proved that the
chemiluminescence hybridization reaction was detecting par-
vovirus B19 sequences specifically. In fact, no chemilumines-
cent signal was observed when B19-positive bone marrow cells
were hybridized with the plasmid pUC18 control DNA labeled
probe and treated with antidigoxigenin Fab fragment conju-
gated with alkaline phosphatase and with the chemilumines-
cent substrate. Similarly, no luminescent signal was detectable
after hybridization with unlabeled probes followed by the im-
munoenzymatic chemiluminescence treatment. Moreover, B19-
positive bone marrow cells were completely negative after hy-
bridization with the B19 labeled probes when the primary
incubation with antidigoxigenin antibody was either omitted or
replaced with incubation with nonimmune sheep serum.
In recent years there has been a growing interest in the
application of in situ hybridization for the diagnosis of viral
diseases, especially for those viruses, like parvovirus B19, that
cannot be diagnosed by isolation procedures. The possibility of
using a nonradioactive label, such as digoxigenin, which has a
sensitivity similar to that of radioactive labels and a better
resolution, has made this technique more attractive for diag-
nostic laboratories trying to avoid problems related to the short
life of radioactive compounds, disposal, and personnel safety.
In this study we have developed a chemiluminescence in situ
hybridization assay for the search of B19 DNA which could
combine the specificity of digoxigenin-labeled probes, the sen-
sitivity of alkaline phosphatase chemiluminescent substrates,
and the spatial morphological resolution of in situ hybridiza-
tion, using a high-performance, low-light-level imaging lumi-
nograph connected to a light microscope and to a computer for
image analysis. Although the technological equipment em-
ployed in this work is mainly a research tool at this time, in our
study we tried to explore its potentiality for diagnostic pur-
poses. In our assay we used digoxigenin-labeled probes which
were immunoenzymatically revealed by using antidigoxigenin
Fab fragments conjugated with alkaline phosphatase. The use
of Fab fragments in the detection of hybridized probes can be
highly specific without any background due to the presence of
cells expressing Fc receptors, and the use of alkaline phos-
phatase avoids many of the disadvantages of horseradish per-
oxidase, such as interference from endogenous peroxidase ac-
tivity in cells and the need to pretreat cells to inhibit the
enzyme. The chemiluminescence detection of the alkaline
phosphatase-labeled antibody was performed with the 1,2-di-
oxetane substrate CDP-star, which represents one of the most
sensitive detection systems, being able to reveal as few as 1.6
zeptomoles of the enzyme (3); moreover, CDP-star has glow-
ing kinetics with a steady-state emission which permits easier
handling of the specimens, and, since the signal intensity is
proportional to enzyme activity or concentration, it also allows
accurate analysis of the samples. In our chemiluminescence in
situ hybridization assay, signal acquisition was done after the
liquid film of the substrate over the section was removed. This
permitted a sensitive and sharp topographical localization of
the signal, as the mobility of the luminescent product within
the liquid film was avoided and the path and scatter of the
photons were as limited as possible. Moreover, processing the
images by using an image superposition function avoided the
problems which may arise from the sequential analysis of the
FIG. 1. Chemiluminescence in situ hybridization revealing B19 parvovirus in
bone marrow cells. From top to bottom: live image, luminescent signal, and
overlay of the live image and luminescent signal.
Page 4
tissue structure and the chemiluminescent signal obtained on
the screen in turn (15).
A sharp topographic distribution of the probe within the cell
was achieved, as the LB-980 instrumentation with the conven-
tional setup has a spatial resolution of 240 ?m, i.e., one pixel
corresponds to this value, but once the instrument is connected
to the microscope and with the use of the 40? and 100?
lenses, pixel size is reduced to 1.1 and 0.41 ?m, respectively.
Our chemiluminescence in situ hybridization to detect B19
DNA in bone marrow cells proved very specific and very sen-
sitive, as all the positive samples from patients with a diag-
nosed B19 infection also proved positive with our assay, with a
larger number of positive cells per sample thus permitting an
easier evaluation of the sample. Moreover, the sample which
had proved positive by in situ hybridization with colorimetric
detection, from a patient who had tested negative for the
presence of B19 DNA by dot blot hybridization and nested
PCR in peripheral blood, was confirmed positive by chemilu-
minescence in situ hybridization.
Moreover, among 24 samples from patients with hematolog-
ical disorders who had proved negative for B19 DNA by in situ
hybridization with colorimetric detection, dot blot hybridiza-
tion, and nested PCR in peripheral blood, chemiluminescence
in situ hybridization confirmed the negative result for 23 sam-
ples and for 1 sample gave a positive result, proving as sensitive
as nested PCR performed on bone marrow cells. Our chemi-
luminescence in situ hybridization thus offers the possibility of
diagnosing B19 infection in a small percentage of the total
number of cells without extraction of viral nucleic acids and
obtaining information on the molecular aspects of B19 infec-
tion, with wide applications for both research and diagnostic
purposes. As the ability of B19 to persist in bone marrow cells
over long periods of time in the absence of detectable viremia
has been demonstrated by PCR directly on bone marrow as-
pirates (4, 6, 8), our chemiluminescence in situ hybridization
could also be useful for monitoring persistent replication of
B19 in bone marrow cells in chronic infections.
The skillful technical help of Marinella Plazzi is gratefully acknowl-
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text:
Re: Journal Entry-Eve, November 20 - Eve
Expand Messages
- --- In meditationsocietyofamerica@y..., "tosime" <tosime@b...> wrote:
> Hi Eve,your
> Thanks for such a beautiful journal entry. I really enjoyed your
> recollection of the holographic image - it was touching.
> How did it feel when the image changed between the willow tree and
> room? Was it you doing the shifting or was it out of your control?Hi Tony,
> ...Tony
I don't recall noticing a difference in how I felt when the image
would change. At first, the double image just happened, but then I
would consciously do it. I found it helpful in keeping my awareness
in the room. I feel it's a good idea to keep some awareness of my
environment when I am meditating because I think it may help when
trying to experince a meditative awareness throughout the day. I
don't know if this is actually true or not, just seems right to me.
>Has this happened before? Was there any connection with you as a kidThis was the first time this happened. There wasn't any connection
>looking at that book?
with being a kid. I just mentioned it to help describe the image,
thinking maybe someone else had one of those books too.
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subset: commoncrawl


text:
This review is long overdue. My husband and I stayed here in May 2012 when we cycled the Shimanami Kaido (which was fantastic!). We found it a lovely, friendly little place. Easy to get to, only a few minutes on the ferry from Onomichi station. There is an elementary school across the road from the house, as we walked by... More
If you own or manage B&B Shiokaze, register now for free tools to enhance your listing, attract new reviews, and respond to reviewers.


text:
Although Basenotes strives to ensure that all of the reviews that appear on the site follow the review guidelines, occasionally one or two reviews may slip through the net.
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Kelly Caleche is quite strange. To my nose, it's a harsh leather scent with pink floral undertones.
I find it quite consistent in being a blend of both softness and sharpness. I applaud Jean-Claude Ellena for being so daring in his approach, however this blend leaves me a little dumbfounded.
I find myself being rather drawn to it, yet its occasional hints of masculine soapiness turns me off it slightly. Especially towards the drydown however, the soapiness vanishes and is replaced with a delicate and feminine powderiness.
Hermes creations often have a very distinguishable vegetal quality in their fragrances. I initially thought that this quality was absent in Kelly Caleche, yet I must admit I was wrong and that the vegetal smell comes across rather strongly with the leather in the opening. I can't say I'm a fan of this smell.
While the leather in Kelly Caleche is mild when compared to the likes of Gres Cabochard and Robert Piguet's Bandit, it is certainly present and quite loud alongside the florals. The longevity is wonderful I must say, and this is for the EDT version.
Overall, I recommend Kelly Caleche based on its uniqueness, however for those that like leather scents to be highlighted by woods rather than flowers, I'd probably skip this.
20th April, 2012
Basenotes is an online guide to perfume and fragrance, featuring news, a database of fragrances, perfume glossary, fragrance forums, user reviews and more.


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Hmm, I'm Wondering Where Are The D4s Low Light Image Samples!
Started Mar 5, 2014 | Discussions thread
Shop cameras & lenses ▾
Contributing Member • Posts: 589 • Gear list
JMD-70 wrote:
I'm really wanting to see some low light comparisons of D3s, D4, D4s and 1Dx.
Should be exciting if done right.-- hide signature --
...D3s/D3/WT-4/Eye-Fi 8GB X2 Pro
......ITP Pro 2.0
.........Nikon forever~
Yes! The Eye-Fi works with D3 and D3s!
Here ya go:
benjaminblack's gear list:benjaminblack's gear list
Nikon D4 Nikon D810 Nikon AF-S Nikkor 24-70mm f/2.8G ED Nikon AF-S Nikkor 70-200mm f/2.8G ED VR II Nikon AF-S Micro-Nikkor 105mm f/2.8G IF-ED VR +5 more
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Featured Video
Calendar of Events
Latest School News
Fine Arts ProgramRead more...
As part of our Fine Arts program, Amazing Grace students have recently attended:
Amazing Grace and Renton Preparatory Christian Schools to Receive New York's CUEM First Annual Lester R. Bayer Award for Excellence in Urban EducationCUEM, located in New York City, is pleased to announce the recipient of the first annual Lester R. Bayer Award for Excellence in Urban Education. Read more...
Celebrating Diversity with an International PotluckStudents, their families, and friends joined together to celebrate the end of the school year by sharing in a potluck.Read more...
Students Sing for DedicationRead more...Amazing Grace and Renton Prep students enjoyed the opportunity to sing at a dedication.
Renton Prep Students Invited to Visit Microsoft Labs and LeadersRead more...Some of our Renton Prep students had an amazing opportunity to visit the Microsoft labs and leadership team.
Renton Prep Students Selected to Attend the 2015 ISTE ConferenceRead more...Big things happen at the ISTE Conference & Expo.
Renton Prep Students at the TheaterRead more...Renton Prep Students attend Othello and Phantom of the Opera.
Renton Prep Awarded 1st Place in Education Design ChallengeRead more...Prep students had the opportunity to submit proposals and participate in an educational learning design challenge at the WIPTTE 2015 Conference.
Stanford University Engineering Course
AGCS 7th and 8th Grade students have successfully completed a two month course from Stanford University.Read more...
Music of Today Series
Amazing Grace 5th-8th grade students attended the Music of Today series on the University of Washington, Seattle campus.Read more...
Princeton University World History Course
AGCS 7th and 8th Grade students have successfully completed a four month World History course from Princeton University.Read more...
STEAM Project Challenge: Build a Bridge!
The Transdisciplinary STEAM Amazing Grace Middle School students collaborated in researching, designing, and constructing a bridge that spans 50 centimeters using a maximum of 20 straws and tape provided.Read more...
University of Virginia Physics Course
AGCS 7th and 8th grade students have enrolled in a University of Virginia Physics Course.Read more...
Stanford University CS 101 Challenges Students
Amazing Grace has enrolled middle school students in several university courses beginning with Stanford University's Computer Science 101.Read more...
Aaron Copeland and George Gershwin Study
Amazing Grace Middle School students studied Aaron Copland and George Gershwin.Read more...
Historic Seattle Paramount Theater Field Trip
See the sights and sounds captured by Amazing Grace Middle School students during their experiential learning activities.Read more...
Harlem Renaissance Study
Amazing Grace Middle School students studied the Harlem Renaissance by utilizing a variety of resources.Read more...
STE(A)M Tall Tower ChallengeAmazing Grace Middle School STE(A)M Tall Tower Challenge project included science, technology, engineering, art, and mathematics.Read more...


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Baz Luhrmann's Miami Hotel
The filmmaker and his wife, the costume designer Catherine Martin, created dramatic, tropical interiors.
The filmmaker and his wife, the costume designer Catherine Martin, created dramatic, tropical interiors.
By JEFF OLOIZIA
By VALERIYA SAFRONOVA
Among its high-priced neighbors, the Croydon is a reasonably priced, convenient and comfortable option with perks that make choosing it worthwhile.
By ONDINE COHANE
The restaurant, in an Art Deco building just a few steps away from Ocean Drive, stands out for its unpretentious vibe.
By SHIVANI VORA
Standard International hotels offer an instant-booking application for same-day room reservations.
By CHERYL LU-LIEN TAN
This property, which opened in April 2013, aims for a retro beach vibe in a quiet nook of South Beach.
By NEIL GENZLINGER
On a cruise between Miami and the Bahamas, live music is just the beginning of the fun.
By SETH SHERWOOD
After a total renovation, this 64-room property reopened last July, featuring colorful, playful details, but minimal glitz.
By STEPHANIE ROSENBLOOM
Shivering in frosty temperatures where you are? Thaw out with a trip to South Beach, where the attractions extend far beyond the warm weather.
By EMILY BRENNAN
Reopened under the James's ownership in November, this property exudes laid-back luxury and offers a host of amenities.
By EMILY BRENNAN
You're never far from the water at this newly reopened property, with a 45-foot-pool on the roof and the beach just across the street.
By KIM SEVERSON, MICHAEL TORTORELLO, JOHN ELIGON and EMILY BRENNAN
At these four restaurants, from Miami Beach to Minnesota, regional flavors are layered with personal stories.


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If you want to see what the new normal looks like when the American Civil Liberties Union (ACLU) calls the shots, look no further than Cranston, R.I. That city of 80,000, the third-largest in the Ocean State, is at the epicenter of the ACLU's war on the normal.
It makes a perverse sort of sense, because Cranston reportedly is the inspiration for Quahog, R.I., the fictitious setting of Seth MacFarlane's vulgar, anti-family Fox TV comedy "Family Guy." Mr. MacFarlane, who has been tapped to host the 85th Academy Awards on Feb. 24, is an avowed atheist who has grown rich by mocking America's core values.
Earlier this year, the ACLU got a federal judge to order a public high school in Cranston to tear down a banner that had inspired students toward exemplary behavior for 50 years. In September, the ACLU managed to get a father-daughter dance canceled on the grounds that it encouraged "gender stereotypes."
In both cases, the community rallied around the schools, but the ACLU ground them down. In the banner case, the ACLU quickly dunned the district $178,000 in legal fees, courtesy of Cranston taxpayers, who already had funded the defense. As in many communities, Cranston district officials did not change heart on the issue — they just wanted to stop the bleeding. This is why a bullying letter from the ACLU often kills resistance before it starts.
Americans have almost grown accustomed to the ACLU using the courts to rip out Ten Commandment monuments, trash city hall Nativity scenes, even force the removal of highway crosses memorializing fallen state troopers, and make schools officially atheist in form and function. But the dance cancellation takes the absurdity to new heights.
So, how did the ACLU justify attacking the father-daughter dance? Apparently the event might indicate that males and females are different. Seriously. Plus, not every girl has a dad or father figure who will take her, so some girls might be left out. Therefore, it's better to cancel the whole thing.
After school district officials threw in the towel, the ACLU applauded them, saying, "The school district recognized that in the 21st century, public schools have no business fostering the notion that girls prefer to go to formal dances while boys prefer baseball games. This type of gender stereotyping only perpetuates outdated notions of 'girl' and 'boy' activities and is contrary to federal law."
No, it's not. Federal law permits exceptions for gender-based events, just as it permits the existence of separate restrooms for girls and boys.
In the other case, a U.S. district judge in January ordered Cranston High School West to take down a prayer banner, which the school did. Despite a flurry of support, including a petition signed by 4,000 people to keep the banner, the school district declined to appeal. Here's the text of the nondenominational school prayer, which had hung in Cranston High School West's auditorium since 1963:
Our Heavenly Father, Grant us each day the desire to do our best,
To grow mentally and morally as well as physically,
To be kind and helpful to our classmates and teachers,
To be honest with ourselves as well as with others,
Help us to be good sports and smile when we lose as well as when we win,
Teach us the value of true friendship,


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Re: Harbin is fun (and the snow is real!)
The nearby ski resort at Yabuli is a fun day trip with cheap ski rentals. My very first skiing experience was at Yabuli, which was also the site of a recent Asian Winter Games. You can bet your boots there will be snow in December.
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Hostel Grand Place
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TravelPod Member ReviewsHostel Grand Place Brussels
This is a good hostel, once you actually get there. I checked in at a different hostel before having to head to the actual one I was due to stay in. Great rooms and great location though
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Waffles, Fries, Chocolate...Belgium
It's been the busiest two weeks of the school year around here; four essays and two midterms due, but I'm here to travel abroad, amirite?
I took a 1.5hour flight to Brussels, Belgium (fun fact: the de facto capital of the European Union ) with Rosalie, an exchange friend from Quebec City. We arrived late Friday night to a dorm room of 8 …
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... the area (H&M and Zara fist obviously) and I even bought another pair of fun pants. That day we unknowingly walked past Mannekin Pis and didn't even think twice about that being the tourist attraction; it was just so small. After wandering around the city for a while we followed a clumsy woman with a yellow balloon to the bus station and took a bus to the Automium and Mini Europe. I did not expect ...
Bittersweet Finale with My Depeche Mode
... to wear eyeliner which left me with the usual fuzzy feeling inside. Rock Werchter was obviously rocking. It wasn't as crazy as I expected it to be even though it was a pretty crowded venue. There were three stages set up but for our purposes we utilized just two of the stages as we went back and forth to see various acts.
As a final date with my DM, it was bittersweet. Truth be told, I have learned to be better at not ...
Brussels - Andrew's lowdown
... Of course there is however no other way to see so much of Brussels without spending a lot more time and investing ourselves in the "derelicte" public transport! Anyhow on our last day we set off to Bruges to check out this nice little Flemmish speaking city and we were glad we did! V v quant and different to Brussels. Nice to wander around. However the two biggest things we did were: Chocolate and Play in the Snow!! The chocolate was everywhere and we spent an obscene ...


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May 11, 2013
Bobby Deren
The safety position loses one starter from last year and will have a different look heading into the 2013 season. Here's a glimpse at how the position looked throughout spring practice. ...More... To continue reading this article you must be a member. Sign Up Now!
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Bill Stenger of Jay Peak Resort said that he should have communicated better with the first investors in his Jay Peak development projects. The news surfaced recently that some of the foreign investors who gave money to build the Tram Haus Lodge at Jay Peak Resort, known as Phase I, expressed concerns with the deal. Stenger modified the agreement and did not tell investors until after the fact. The move was not illegal, but according to best practices the investors should have been told ahead of time. Investors also became concerned that they may not get their money back. Â
"I sincerely apologized for the slow and inadequate communications," Stenger said Tuesday.
For the rest of the story see Wednesday's Express!


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PDT and Periodontal Treatment in DMT2 Patients (PDTDMT2)
- Full Text View
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- Disclaimer
- How to Read a Study Record
The relationship between diabetes mellitus (DM) and periodontal disease is bidirectional. DM is a predisposing and modifying factor of periodontitis, which, in turn, worsens glycemic control and increases proteins found in the acute phase of inflammation. The gold standard for the treatment of periodontal disease is oral hygiene orientation, scaling and planing. Moreover, systemic antibiotic therapy may be employed in some cases. In an effort to minimize the prescription of antibiotics, photodynamic therapy (PDT) has been studied as an antimicrobial technique and has demonstrated promising results. The aim of the proposed study is to determine whether PDT as a complement to periodontal therapy (PT) is helpful in the metabolic control of individuals with type 2 diabetes and the reduction of acute phase inflammatory markers. The patients will be randomized using a proper software program into two groups: 1) PT + placebo PDT or 2) PT + active PDT. All patients will first be examined by a specialist, followed by PT performed by two other healthcare professionals. At the end of each session, PDT (active or sham) will be administered by a fourth healthcare professional. The following will be the PDT parameters: diode laser (660 nm); power output = 110 mW; exposure time = 90 seconds per point (9 J/point); and energy density = 22 J/cm2. The photosensitizer will be methylene blue (50 µg/mL). The patients will be re-evaluated 15, 30, 90 and 180 days after treatment. Serological exams with complete blood count, fasting glucose, glycated hemoglobin and crevicular fluid exams to screen for tumor necrosis factor alpha, interleukin 1, interleukin 6, osteocalcin, osteoprotegerin/RANKL will be performed at each evaluation. At baseline and 180 day periapical radiographs will be performed to evaluate the alveolar bone crest level. The data will be statistically evaluated using the most appropriate tests.
Diabetes Mellitus Type 2
Procedure: Periodontal treatment
Procedure: Periodontal treatment and PDT
|Study Type:
|Study Design:
|Allocation: Randomized
Endpoint Classification: Efficacy Study
Intervention Model: Parallel Assignment
Masking: Double Blind (Subject, Caregiver, Outcomes Assessor)
Primary Purpose: Treatment
|Official Title:
|Combination of Photodynamic Therapy and Periodontal Treatment in Patients With Type 2 Diabetes Mellitus: Randomized, Double-blind Clinical Trial
- Change in the clinical attachment level at 180 days. [ Time Frame: 180 days ] [ Designated as safety issue: No ]Clinical attachment level is the depth measured from the CEJ (cement-enamel junction) to the bottom of the gingival sulcus. It indicates the amount of attachment that has been lost.
- Changes in the fasting blood and glycated hemoglobin (HBA1C) [ Time Frame: Baseline, 15, 30, 90 and 180 days ] [ Designated as safety issue: No ]These tests reflects the glycemic control at the moment of the dental exam and the average blood sugar level for the past two to three months.
- Cytokines in the crevicular fluid [ Time Frame: Baseline, 15, 30, 90 and 180 days ] [ Designated as safety issue: No ]The crevicular fluid will be collected and investigated for the presence of IL-1, IL-6, TNF-alfa, osteocalcin, osteoprotegerin/RANKL in the crevicular fluid.
|Estimated Enrollment:
|Study Start Date:
|October 2013
|Estimated Study Completion Date:
|December 2016
|Estimated Primary Completion Date:
|December 2015 (Final data collection date for primary outcome measure)
|Assigned Interventions
Sham Comparator: Periodontal treatment
Conventional periodontal treatment with hygiene orientation, calculus removal and root scaling and planing. Sham PDT.
Procedure: Periodontal treatment
Periodontal treatment will consist of oral hygiene instructions, with the monitored orientation of brushing technique and recommendation of the daily use of dental floss. Calculus deposits will also be removed using ultrasound equipment with the appropriate tips for periodontics as well as scaling and planing with dental instruments, as recommended by the AAP (2001). Treatment will be carried out in two to four one-hour sessions.
Patient will receive sham PDT.
Experimental: Periodontal Treatment and PDT
Conventional periodontal treatment with hygiene orientation, calculus removal, root scaling and planing. Active PDT with diode laser and methylene blue photosensitizer
Procedure: Periodontal treatment and PDT
Patients will receive periodontal treatment as previously described and PDT. PDT will be administered at the end of the periodontal treatment session at sites with pocket depths equal to or greater than 4 mm. A diode laser will be used at the 660 nm wavelength, P= 100 mW, 9 J/point, D = 22 J/cm2 (per point). The photosensitizer (BlueMet 0.005 % DMC)will be deposited at the fundus of the periodontal pocket using a syringe. The laser will be applied to the mucosa over the oral epithelium that lines the periodontal pocket using an optical fiber.
Detailed Description:
Randomization of the patients to the different groups will be performed with the appropriate software program. Only the lead researcher will have access to the randomization list. Allocation to the two groups will be carried out in blocks of four.
The patients will be sent to Operator A for the periodontal exam. Without knowledge of the results of this exam, the patients will be treated by Operators B and C for periodontal treatment, which will involve oral hygiene instructions as well as supra-gingival and sub-gingival scaling and planing in up to four sessions. At the end of each session, the patients will be sent to Operator D, who will administer either placebo or active PDT based on the content of the sealed envelope. Thus, both the patients and operator in charge of the periodontal exams will be blinded to the PDT protocol (double-blind study).
Fasting blood sugar and glycated hemoglobin exams will be performed at baseline as well as 15, 30, 90 and 180 days after the completion of treatment. All exams will be carried out at the same laboratory affiliated with the university.
The clinical periodontal exams will be performed by a single examiner specialized in periodontics, who will evaluate six regions of each tooth with a 15-mm probe (North Carolina model, Hu-Friedy, Chicago, IL, USA). The following aspects will be recorded: plaque index, bleeding upon probing, probing depth, gingival recession and attachment loss (Armitage, 2004). Evaluations will be carried out performed at baseline (prior to initial treatment) as well as 15, 30, 90 and 180 days after the completion of treatment.
Patients will receive periodontal treatment and photodynamic therapy (active or sham depending on the allocation).
Crevicular fluid will be collected from the previously defined sites with a probing depth equal to or greater than 4 mm that will receive treatment. Collection will be performed with relative isolation using cotton rolls following the removal of supra-gingival plaque with periodontal curettes and air drying for five seconds. Absorbent paper strips (PerioPaper, ProFlow Inc., Amityville, NY, USA) will be individually inserted into each site for 30 seconds (Rudin et al., 1970). The collections will be performed by a single researcher. The volume of crevicular fluid samples will be measured using the Periotron 8000 micro-moisture meter (Oraflow Inc., NY, USA). The samples will then be placed in duly identified sterile plastic microtubes, temporarily stored in dry ice and subsequently stored in a freezer at -80 ºC
|Ages Eligible for Study:
|18 Years and older
|Genders Eligible for Study:
|Accepts Healthy Volunteers:
Inclusion Criteria:
- Compensated type 2 diabetes mellitus or with adequate control based on the criteria of the Brazilian Society of Diabetes (SBD, 2012);
- Chronic periodontitis (AAP, 2001);
- Presence of at least 15 teeth and at least four teeth with bleeding upon probing and probing depth greater than 4 mm;
- Aged 18 years or older;
- Under follow up with an endocrinologist.
Exclusion Criteria:
- Uncompensated diabetes, based on SBD criteria;
- Smoking habit in 12 months prior to treatment;
- Anemia;
- Active cancer;
- Current pregnancy;
- History of antibiotic therapy in previous six months;
- History of anti-inflammatory therapy in previous three months;
- Clotting disorder (use of anti-coagulant, presence of liver disease, thrombocytopenia and immunosuppression);
- Currently undergoing orthodontic treatment.
Please refer to this study by its ClinicalTrials.gov identifier: NCT01964833
|Contact: Cristiane M. Franca, PhD
|55 11 9 8244 1343
|Universidade Nove de Julho
|São Paulo, SP, Brazil, 01504015
|Contact: Cristiane M Franca, PhD 55 11 98244 1343 email@example.com
|Principal Investigator: Cristiane M Franca, PhD
|Principal Investigator:
|Cristiane M Franca, PhD
|University of Nove de Julho
No publications provided by University of Nove de Julho
Additional publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
|Responsible Party:
|Cristiane Miranda Franca, Professor of the Post Graduation Program of Biophotonics applied to Health Sciences, University of Nove de Julho
|ClinicalTrials.gov Identifier:
|NCT01964833 History of Changes
|Other Study ID Numbers:
|375.454, 375.454
|Study First Received:
|October 10, 2013
|Last Updated:
|October 14, 2013
|Health Authority:
|Brazil: National Committee of Ethics in Research
Keywords provided by University of Nove de Julho:
clinical trial
Additional relevant MeSH terms:
Diabetes Mellitus
Diabetes Mellitus, Type 2
Endocrine System Diseases
Glucose Metabolism Disorders
Metabolic Diseases
Mouth Diseases
Periodontal Diseases
Stomatognathic Diseases
ClinicalTrials.gov processed this record on October 07, 2015


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Hello support - My "upload a photo" function has disappeared from my Write/Post and Write/Page dashboard tabs.
I just uploaded Photo Album for WordPress. The gallery page on my blog now works like gangbusters. But the plugin is also supposed to "add a new Photos tab in your WordPress edit screen which will allow you to easily insert your Flickr photos into your blog posts." Alas, it seems to have wiped out the ability to add upload/add photos to my posts altogether. :(
Didn't find anything helpful on the plugin's support site. My host is dream host, theme is Swirl, WP 2.0, http://www.uppityrib.com.
Can anyone help? Thanks!


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Bring the true meaning of Christmas into your home with the Gifts of the Three Kings: actual gold, frankincense and myrrh nestled together in this beautiful collection.
Recommended by Glenn Beck. Sharpen your own Constitutional savvy while you teach your kids!
From producer Stephen McEveety ("The Passion of the Christ") comes an amazing documentary on the star of Bethlehem.
It was the cruelest of times. Under Herod's torturous reign families struggled to survive, and yet, in the midst of utter turmoil, a young woman's faith is put to the test. Join Mary (Keisha Castle-Hughes) and Joseph (Oscar Isaac) on an incredible journey of hope and discovery.
Daniel has nothing but his faith to stand between him and the lions' den in this top-selling film
Messianic Rabbi Jonathan Cahn looks at the evidence and finds historical confirmation for the Resurrection of Yeshua in an unlikely source – the rabbinical writings of the Sanhedrin itself.
Mystical death curses. Visions of Messiah. A renowned world political leader under the threat of death. Religious leaders in absolute shock. A cryptic death note ...
The 9/11 Anniversary NEVER FORGET Challenge Coin keeps the memory of those who died on 9/11 forever alive in our hearts and minds.
Accent your faith with a beautiful blossom from the Holy Land! Handcrafted in matte platted gold this cross design is set with the Rose of Bethlehem, a flower that repeatedly blooms with just a few drops of water.
Just as James Sundquist transports listeners with his lyrics, he transports listeners to another epoch by recording instrumental versions of well-known hymns and carols sung by saints throughout the ages.
Made of fine Nappa leather this RFID wallet protects your credit card info from "skimming" attacks.
Easy access to clean and safe drinking water anywhere! Filters at least 1,000 liters of water and removes up to 99.9% percent of waterborne bacteria
The wild party game that provides instant fun for everyone. Fast-moving and refreshing, Apples to Apples is perfect for any get-together with family and friends!
A charming and fun Christmas puzzle to put together on a cold winter day.
"Scandal" is the perfect inexpensive gift with a message that all conservatives can appreciate.
Officially licensed U.S. Marines mink blanket. Super soft with bright vivid colors
Vivid graphics and colors on 100% brushed twill cotton
The Hot New Releases Gift Box includes current top sellers. Limited availability.
The Rabbi Who Found Messiah: The Story of Yitzhak Kaduri and His Prophecies of the Endtime (Autographed) (Hardcover)
Mystical death curses, visions of Messiah, an important world political figure under threat, religious leaders in shock, a cryptic kabbalistic death note ...
50 Years later, stunning new revelations about the JFK assassination
Celebrate our Savior's birth with this simple yet beautiful silicone bracelet.


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Your shortcut to Asia
Fly the short northern route
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Finnair is Europe's first airline to fly the new, fuel-efficient and quiet Airbus A350 XWB*. Flights with the A350 aircraft start on 21 November 2015 to Shanghai. The following routes will be Beijing, Bangkok, Hong Kong and Singapore. The dates for the other routes will be announced soon. Routes to Bangkok, Hong Kong and Singapore will be flown with a mix of A350 and A330 aircraft. Please note that it is shown as A350-900 in the Finnair booking engine.


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Burak Angunes Photographer
Sephora Marie SylverneModel Female Miami, Florida, US
My Website: FacebookMy MM URL: http://www.modelmayhem.com/SephoraMS
Mayhem # 1451166
About MePetite Female, Born in the Carribean, just guess which Island? I am sexy without being provacative, I am very personable, & intelligible. Am open to most shoots *EXCEPT* Erotic, Full Nudity, Fetish. I am not 6'0 tall, but I have a tall personailty to compensate, and an ability to endure alot to get the shot (ask any photographer I've worked with). My job is to sell, as a model I try my best to make any and every product desirable and therefore profitable. Easy-going but a seriously hard worker.
If you are a professional and want TF work with me I'd be glad to shoot with you. I am more into acting than modeling so I want more commercial, lifestyle looks, but I am willing to do some creative projects to feed my inner artist, just send me details.
Themes for shoots I'd like to do for 2010, I plan on beefing up my port with alot of variety. Here are some, not all, but some ideas I'd love to do:
*Enchanted Woodland Fairy theme (Anything ethereal)
*Abondoned sites (buildings, airplane, train, or other non working equipment graveyards)
*Underwater (Although I can't really swim so has to be close to shore, in a flowy dress or swimwear)
*Underneath pier docks (swimwear)
*In an old fashioned car
*Couple shots (some intimate [NON-NUDE, maybe lingerie] some playful commercial shots, MF or FF)
*Editorial group shots
*Business attire/ Grunge business attire
*Vintage/Period shoots (medieval, forties, sixties etc.)
*Graffiti background with crazy outfits like a big tulle dress
*Wedding dress shots
*Alot of fitness/lifestyle/commercial/editorial
*Silhouettes doing dancing or yoga poses (outdoor w/ sunset)
*Bodypainting but full body coverage
All artists interested in doing these kind of shoots, please contact me I would love to collab. with you!
CreditsRobert Frederick
Ronnin Marco
Juan (Miramodels)
Philip (Kontast Photos)
KG Photography
KP Photos
Rupert Taylor Photography
Burak Angunes Photographer
Lara Marenco Model
PhotogJB Photographer
Zakiyyah05 Model
Steve Pereira Photographer
ragamufyn Clothing Designer
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Stacy-Ann Goodenon November 29, 2014 | no comments
After the big Thanksgiving feast and the kids are down for the count, my husband and I lie awake in bed chatting about what we're thankful for. We're grateful for our family and our health. We have two beautiful children, and agree that being a parent isn't always easy. There are sleepless nights, moments when… Read more »
Melissa Willetson November 27, 2014 | 49 comments
I had my pregnancy reveal all planned out. And it was going to be perfect! It was set to go down at our Thanksgiving dinner table, set with my best china and crystal, candles lit, the wine poured (mine was a decoy, te-he), turkey about to be carved, a fire blazing in the hearth behind us. I… Read more »
Whitney Barthelon November 26, 2014 | 3 comments
Sometimes being a mom is just plain hard. It is easy to lose the joy in taking care of little ones if you aren't careful. Trudging through life focusing on tedious day-to-day tasks can make you lose sight of what is important. This Thanksgiving I am making a real effort to take a step back… Read more »
Krissie Schmidton November 26, 2014 | 6 comments
Thanksgiving is fast approaching. I'm the kind of person who is usually grateful all year round but last year I was not feeling particularly grateful. Thanksgiving was only a few months after my son passed away and though I surprised myself by choosing to participate in the holidays, in hindsight I probably should not have…. Read more »
Melissa Byerson November 26, 2014 | no comments
"I'm so busy!" It's not the new black but still, it's what my life has become. I always swore we'd never be one of those overcommitted families where every member is going off in separate directions. I vowed we'd eat dinner together every night. I promised myself we were different – and we were until… Read more »
Carolyn Robertsonon November 24, 2014 | 7 comments
I always find fall to be a completely crazy and overwhelming time of year. The days are too short, too dark and too cold. The kids are back to school and everything else. That calendar that sat wonderfully empty all of those long summer days is suddenly bursting at the seams. And in the midst… Read more »
Sara McGinnison November 23, 2014 | one comment
Jennifer Garner and Ben Affleck have big plans to spend this Thanksgiving with another celebrity family. Violet, Seraphina and Samuel will have a quartet of playmates to hang out with — the four daughters of Matt Damon and wife Luciana! Ben recently opened up about their holiday plans by sharing, "My mother is staying with… Read more »
Lindsay Weisson November 20, 2014 | 2 comments
Because you know I'm all about those PIES ('bout those pies) No turkey I'm all about those PIES ('bout those pies) No turkey (Do you think I've been listening to top 40 radio too much?) I love pie. I love it so much that I've compiled a list of all the amazing pies you need… Read more »
Jamielyn Nyeon November 14, 2014 | no comments
Thanksgiving is a time for delicious food, spending time with family, and expressing gratitude for our blessings. In my family, we always share a few things we're grateful for as we enjoy our Thanksgiving meal together. It's an easy way to teach kids the importance of appreciating what we have. What are your Thanksgiving traditions?… Read more »
Kami Bigleron November 11, 2014 | one comment
Part of what makes Thanksgiving wonderful is the time we spend together with family. Each year we try to make the most of the opportunity by doing some special activities with the ones we love. Sometimes it may be a craft project that we make with our intermediate family to express our thankfulness. While other… Read more »
Lindsay Weisson November 11, 2014 | 5 comments
Side dishes and I don't really get along. I loathe them really. I mean, it's enough work for me to get a main dish on the table every night, never mind a bunch of extra food, extra work and extra dishes. I am the queen of one-pot meals where everything is thrown in together. No… Read more »
Kami Bigleron November 4, 2014 | 2 comments
It's hard to believe it's already November, but Thanksgiving is almost here. It's such a wonderful time to celebrate family and all our many blessings. But it's also hard not to get caught up in the craziness of the season. So, take a deep breath and let me help you with some of the planning…. Read more »
Jamielyn Nyeon November 27, 2013 | 2 comments
I'm sure many of you are prepping for Thanksgiving today like I am. There's lots to do: Set the tables out, bake the pies and clean the house. I've been making several lists and checking them twice. One thing I just realized I am missing is a centerpiece for my table. Thanks to Pinterest, I… Read more »
Leah Speeron November 27, 2013 | no comments
Thanksgiving can be stress-free. It just takes a little planning, a little flexibility and of course, a little lot of wine. My mother modeled seamless Thanksgiving dinners filled with family and friends; scrumptious hors d'oeuvres and homemade desserts; laughter and love. Not a suggestion of stress in the house. As I grew older, I would soon… Read more »
Laura Larsenon November 27, 2013 | 8 comments
Happy Thanksgiving! We give thanks to all our wonderful Community members who have shared pictures of their darling little ones with us! Peek through the slideshow to see twenty little turkey entries from our 2012 Thanksgiving Babies contest. Want to get in on the fun? My blog photo is from BabyCenter's 2013 Thanksgiving Babies photo… Read more »


text: Uniform Crime Reporting Program Data [United States]: Property Stolen and Recovered, 2005 United States Department of Justice. Federal Bureau of Investigation arrests crime rates crime reporting crime statistics larceny law enforcement offenses property crimes property crime statistics stolen property stolen property recovery Uniform Crime Reports ICPSR.XVII.E NACJD.VIII Since 1930, the Federal Bureau of Investigation has compiled the Uniform Crime Reports (UCR) to serve as periodic nationwide assessments of reported crimes not available elsewhere in the criminal justice system. Law enforcement agencies contribute reports either directly or through their state reporting programs. Each year, this information is reported in four types of files: (1) Offenses Known and Clearances by Arrest, (2) Property Stolen and Recovered, (3) Supplementary Homicide Reports (SHR), and (4) Police Employee (LEOKA) Data. The Property Stolen and Recovered data are collected on a monthly basis by all UCR contributing agencies. These data, aggregated at the agency level, report on the nature of the crime, the monetary value of the property stolen, and the type of property stolen. Similar information regarding recovered property is also included in the data. 2007-07-10 aggregate data 20160 10.3886/ICPSR20160.v1 United States 2005 ICPSR metadata records are licensed under a Creative Commons Attribution-Noncommercial 3.0 United States License (http://creativecommons.org/licenses/by-nc/3.0/us/).


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Natural Products Expo West, 2010…Saturday Before Goin' In…
Alright…the bus got me here in under two hours. I got myself to the press room, needing some time to pull my act together before going in. Had a late night writing and such. Needed to perk up and put my game face on. While in the press room, had a great chat with Kelly Gallagher, a local producer whom I've been seeing everywhere lately…have been buddies on facebook for a while, but just met in person recently at Conscious Life Expo. Need to follow up on that connection, for sure!
Yesterday, I felt terrible about chucking my coffee cup after gulping down a cup of joe, so I brought my own mug this time. Wish I didn't need the coffee, but that's my reality for today. Couldn't go in looking and feeling low on energy. Gotta sparkle, shine, give a good show.
Was wondering what Starbuck's coffee is doing in the press room? Claire Tindall told me that it's contracted through the convention center. Her company was hoping that someone (an organic and more conscioulsy-minded coffee company) would step up to the plate and sponsor the coffee in the press room. Guess it didn't happen this time around, but come on organic coffee people! This could be a great opportunity to show your support, spread awareness and promote your products! Maybe next time…
Anyhoo, a little coffee, some laughs, a chat with the ladies behind the scene in the press room, some time to put my game face on, and I'm almost ready to go. Am feeling like I do before a stage performance…jittery, heart jumping like something big is about to happen. Is it the coffee, or IS something big going to happen here for me? I mean, in addition to the reporting gig? We shall see! In any case, it's an honor to be reporting here again for Elephant Journal.
I took a little time to check out the display cases today to pull together a game plan. I'm planning to visit the supplements, "natural living" and bodycare sections today. Will continue to keep my eyes open for things of interest, taking more photos, getting the inside scoop. If you haven't read my other two posts yet, make sure to do so. Type in "Expo West Claire Amber" in the Elephant Journal search box. Wish I could feed you the links, but am using the computer at the Expo.
Alright, I'm going in. Wish me luck and stay tuned for more! Cheers!
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La Conchita civil trial opens with statements
Wrongful death, damage case stems from 2005 landslide
Engineers, hydrologists and other experts will testify in the wrongful death and property damage civil trial that was triggered by a 2005 landslide in La Conchita, but the case is really quite simple, a plaintiffs' lawyer told jurors Monday.
During opening statements in Ventura County Superior Court, attorney Anthony Murray said there will be complex testimony about the effect of water coming down a mountainside and landslides.
"But in the end you will find that this was rather a simple case," he said.
He said the "perfect recipe" for creating a landslide is putting a lot of water into an unstable slope.
The trial was triggered by an eight-second landslide that killed 10 people and destroyed 13 homes in the seaside community.
The trial could last up to four months and has a list of more than 200 potential witnesses, including experts, residents and county officials.
Nearly all of the plaintiffs were in court Monday and were introduced to the jury by Murray.
Accusations of poor drainage
Murray's firm, Loeb & Loeb of Los Angeles, is representing the plaintiffs. They include homeowners, residents of La Conchita, injured parties and the families who died.
Murray claims that La Conchita Ranch Co. saturated its orchards with water and didn't build an adequate drainage system.
This, he said, was coupled with the county building a retaining wall in 2000 at the base of Rincon Mountain.
Rainwater and irrigation water from the ranch was dammed up by the wall, he said. When the pressure got too great, the wall came tumbling down and onto the houses and streets, Murray told jurors.
He said the landslide couldn't have happened without the negligence of both the ranch company and Ventura County.
Attorney Richard E. Morton, who is representing the ranch company, described La Conchita to jurors as a "weakly cemented bunch of rocks" that was formed more than 40,000 years ago.
He said this 50-acre area has a history of landslides and mudslides. He said there have been many geologic and engineering studies as early the late 1800s.
"It is an area that is vulnerable to landslides and continues to be," Morton told jurors.
He detailed six landslides that have happened there through the years, including a 1909 landslide that killed four railroad workers.
Morton's law firm, Haight Brown & Bonesteel, which has offices in San Francisco, Los Angeles and Santa Ana, is representing the ranch company.
Attorney Robert Ceccon of Los Angeles is representing the county and is expected to make his opening statements today.
Jury to decide negligence
The first witness is expected to testify today. A jury of 12 people and six alternate jurors will hear the case and decide only whether La Conchita Ranch Co. is negligent. Judge Vincent O'Neill, who is presiding over the trial, will decide whether the county is negligent.
During opening statements, Murray tried to portray David Orr, president of Vista La Conchita Corp. and the person who runs the ranch and reports to the corporation board, as a man who showed little interest.
Murray said Orr didn't do much after a March 4, 1995, landslide that destroyed or damaged seven houses.
The 700-acre ranch sits on a mountain terrace and grows lemons and avocados.
Murray said the ranch could have put in 30-inch drainage pipes, which could slow the flow of the water. But the ranch axed that plan and instead bulldozed "directly across the face" of the 1995 landslide to dig a dirt channel, which wasn't lined with a plastic substance to keep water out. Murray said it took "several thousand dollars" and "two hours" to do that work.
Morton told jurors that the ranch company did a lot to resolve the problem, including warning residents of its experts' finding when large cracks began to appear in 1994.
After the 1995 landslide, some residents sued the ranch, Morton said. He said some of the same people named as plaintiffs in the 1995 lawsuit, which was settled out of court, are now plaintiffs in the lawsuit involving the 2005 landslide. People who knew about the 1995 landslide also are plaintiffs in the current lawsuit, said Morton.
After 1995, the county declared La Conchita a "geological hazard area," and structures had to post warning signs that also stated: "Enter at your own risk."
The ranch company's net income from 1996 to 2004 was $1.4 million, and it spent $2.3 million working on the drainage and maintaining it and trying to figure out what caused the 1995 slide, according to Morton.
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Where Time is a Frequency and Space is a Holographic Unity
Posted by RuBen SkyWalker on September 19, 2015 at 7:00pm — 2 Comments
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Welcome to Galactic Spacebook – Where Time is a Frequency and Space is a Holographic Unity!
The Mission of Galactic Spacebook is to evolve Galactic Culture on Earth.
As an evolving network, Galactic Spacebook was created to help us Unify in Time on the Return Journey to our Greater Universal Nature – a New Mind, a New Consciousness, a New Way of Life in universal attunement with the Earth and the Cosmos.
This site is a space for positive co-creation based on the Law of Time; and a place of mutual encouragement and support for Star Travelers, Time Beings and Planetary Artists to recover our cosmic memory and unite in a new frequency: 13:20.
The 13:20 frequency is a bridge to assist our emergence from the dense space of linear time to the magnificent simplicity and cosmic magic of natural time.
The 13 Moon calendar (synchronometer) is the key we use on Earth to access the vast realm of the synchronic order.
The positive unification of our minds and hearts on behalf of Galactic Culture sends a powerful ripple through the galaxy and into the noosphere.
It is up to us to create the positive/parallel alternative universe based on Love, Art and Synchronicity!
Vision ♦ Prayer of the Seven Galactic Directions ♦ Intergalactic Premise
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Hello Kin,I wanted to share this link with you all because of the profound impact it had on me and my own spiritual awakening a few years ago. I always think of David as a "homie", in that as spirit he is helping to enlighten those who are aware of…Continue
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What time in Denver, Colorado is Monday's event???
Started by Jed Dowd in 13 Moon Calendar Aug 9.
Hi everybody,https://youtu.be/KArdY3l4xKIi came across this video 2 days ago, and it makes alot of sense to me.Only it makes it seem that the 260 tzolkin calander is out of date,I am very curious for other…Continue
Tags: patern., orbit, shifting, time
Started by pascal Romeijn in Law of Time Aug 9.
all society is actually discordian. look around at our world?…Continue
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Anyone else interested in this event?! http://spacestationplaza.com/event.php?evid=103545&sesset=1Continue
Started by Alicia in General May 28.
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Milly J's Shoe Designs
Please be advised that all basic shoes are sourced from highly reputable shoe makers and then Milly J does her delicious work on them. Milly J ensures that all shoe scructures are long wearing, comfortable and well fitting if an Art to wear pair and robust if a sculpture.
Milly J never compromises class, execution, detail, playfulness and quality for a cheap price that may sell...read more


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I am more interested in meeting an interesting woman than I am in meeting a "nice, warm-hearted" woman. Nice is nice but interesting is....interesting. Interesting conversation is hard to find. And "warm-hearted" makes me think of my Bubbie Zelda who smelled of mothballs and death.
I joke around a lot. Sometimes people don't laugh. I like that.
I am in okay shape for my age. I'm skinny, actually, a 52 year-old skeleton. That makes me an anomaly in today's super-sized USA. Perhaps I should go to McDonald's more.
I work for a television station. That means I can afford to get two toppings on my pizza. Not three, though (if you only knew what tv pays).
I no longer own a car. This is by choice. I had a car. It was terminally ill. I chose to not pay its medical bills. I gave it to a homeless guy. Now he has an Accord to call his home and I ride the bus with toothless people.
I am Jewish. That said, I am not religious. I am not a believer. I can appreciate great fiction, though.
I love children, I just don't want any. Your kids? They are truly wonderful! I don't want them, either. But if you'd like to sell them, I know a guy.....
I also love animals. I like them more than many humans I meet, even when they crap on the floor (the animals I mean, although there was this one time....).
I think some people are so lonely that they'll go out with just about anyone. I find that sad. I mean, just buy yourself some "toys" and get a pet.
I believe your entertainment choices reflect who you are. Your favorite singer is Toby Keith? You like Adam Sandler movies? I'm sorry! Also, we probably won't be compatible. Me? I have very eclectic tastes. Right now I'm listening to Rodrigo y Gabriela, New Pornographers, Sleater-Kinney and Pink Martini. I watch "Louie," "Episodes" and "Mad Men." I loved "The Sopranos," "Breaking Bad" and "Arrested Development." I used to watch a lot of foreign films until they started to feel....foreign . I thought "12 Years A Slave" needed more laughs,
So who am I looking for? A smart cookie. An oddball. Someone who can make me laugh (no mean feat). Someone who's not all about money. Someone who's in decent shape for her age. Someone who's different (but not in a "Fatal Attraction" bunny boiling kinda way).
Someone who read my profile and went, "What the f....?" But who laughed.
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Spark Networks USA, LLC does not conduct background checks on the members or subscribers of this website. However, the safety and security of our members is our top priority. We urge all members to follow our online safety tips.
The Color Code icon tells you that this member has taken the FREE Color Code Personality Test and wants to share his/her results with you. You can read all about their Color Code profile on the new Color Code tab.
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Sometimes it is difficult to choose from the four options given, but there is usually one that fits better than the rest, or at least one that you could see yourself as more so than the others. If you're still unsure, try asking someone who knows you well, such as a parent or sibling. They can give clarity on areas of your personality that you may not notice.


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Westmoreland High School
Public School | Grades 9-12 | Sumner County School District
Westmoreland, TN 37186
(615) 644-2280
About This School
Westmoreland High School is located in Westmoreland, TN and is one of 10 high schools in Sumner County School District. It is a public school that serves 537 students in grades 9-12. See Westmoreland High School's test results to learn more about school performance.
In 2011, Westmoreland High School had 14 students for every full-time equivalent teacher. The Tennessee average is 15 students per full-time equivalent teacher.
In 2011, Westmoreland High School had 14 students for every full-time equivalent teacher. The Tennessee average is 15 students per full-time equivalent teacher.
Student Ethnicity (2011)
Students (2011)
Students Per Teacher (2011)
Female/Male (2011)
49% 51%
Subsidized Lunch (2011)
District Spending (2010)
$7,444per student
See All Test Scores
All Grades U.S. History Performance
TCAP Gateway/End-of-Course Results
write a review
Westmoreland High School Reviews
By a Parent on
Even if we lived anywhere else in Sumner County I would pay out of district for my children to come to this high school. It has great teachers who really care about the students. My son who is currently attending is a straight A honors student there and I am very proud of him. It is a great school!
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Photography blog + iPhone
Multimedia photojournalist John D McHugh discusses his new Marksta app, which adds watermarks to photographs before they are shared on social media
After 52 weeks of diligent smartphoning, we come to the end of a project to test the limits of iPhoneography and document the year in pictures. Hannah Waldram looks back
Karin Andreasson: A growing number of photojournalists are using smartphone apps to tell their stories. Should we see that as a bad thing?


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text: Albert Hall | IBDB: The official source for Broadway Information IBDB: The official source for Broadway Information Official Broadway League sites: - sites - broadway.org broadwayleague.com broadwayfanclub.com kidsnightonbroadway.com stagespecs.org tonyawards.com Quick Search For: In This Category: All Show People/Org. Theatre Season Albert Hall Performer Male Productions ProductionsDate of ProductionsScarlet Pages[Play, Drama, Original] Performer: Albert Hall [Court Stenographers]Sep 09, 1929 - Nov 1929Bewitched[Play, Original] Performer: Albert Hall [A Native of the Enchanted Forest]Oct 01, 1924 - Oct 1924Earl Carroll's Vanities [1924][Musical, Revue, Original] Performer: Albert Hall [Performer]Sep 10, 1924 - Jan 03, 1925 Find out where Albert Hall and are credited together © 2001-2015, The Broadway League, All Rights Reserved. (Copyright and Disclaimer)


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Poppy (Hawkins) is a London primary school teacher. After her bike is stolen she gets driving lessons, but her teacher, Scott (Marsan), turns out to be a bit of a nut. She begins learning flamenco. One of the kids in her class has problems at home. And all the while, she barely stops smiling…
Mike Leigh draws a bravura performance out of Sally Hawkins, and she in return makes Poppy one of Leigh's best characters yet - up there with the likes of Vera Drake or Beverly from Abigail's Party.
Reviewed by Dan Jolin
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Punctuality suffers due to his willingness to take time and truly listen to his patients. Refers patients to Top-Notch specialists when need be. Highly competent, good listener, addresses all your concerns.
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W. Stahle is a professor of Physical Geography and the
Conservation of Natural Resources at the University
of Arkansas. He received his B.A. in Anthropology from
the University of Arizona in 1973, his M.A. in Archaeology
from the University of Arkansas in 1978, and his Ph.D.
in Geography from Arizona State University in 1990.
research interests include all aspects of dendrochronology
(the study of tree rings), particularly climate change
and the proxy evidence for past variation in the El
Nino/Southern Oscillation and other large scale atmospheric
circulations. He has developed GIS-based predictive
models for the location of ancient forests, and is conducting
active research in the United States, Mexico and Africa.
Stahle's research is funded by NOAA, NSF, NPS and the
USGS and he has published in a variety of journals including,
Science, Nature, Journal of Climate and Bulletin of
the American Meteorological Society.
links to David Stahle's home page and other related infomation
please see our resources
responds :
are many sound arguments against the logging of our
last remaining old-growth forests in the USA, but the
threat of global climate change is probably not the
strongest. The remaining old-growth forests constitute
just a small fraction of the country, and the USA constitutes
just a small fraction of the land area of earth. So
logging what's left of old growth in the USA, bad though
that would be, probably would not have a big impact
on global climate, particularly if these ancient forest
sites were allowed to regenerate into some kind of forest
cover. It should be acknowledged, however, that net
deforestation worldwide is a different matter. Climate
simulations have indicated that replacing the triple
canopy virgin rainforest in Amazonia with cattle pasture
would probably indeed have a drastic impact on the regional
climate, and these changes would surely alter the global
atmospheric circulation as well.
rings are shedding new light on several historical mysteries.
White spruce trees from Alaska indicate that the coldest
summer in 400 years occurred in 1783. This unusual cold
was probably brought on by the 1783 eruption of the
Laki volcano in Iceland, and appears to have been responsible
for widespread starvation and population decline among
the Inuit during the "Time summer Did Not Come" (Gordon
Jacoby and others, Quaternary Science Reviews, 1999).
16th century "megadrought" across North America (roughly
extending from 1540-1590) has been linked with the disappearance
of the English Lost Colony of Roanoke Island (1587),
the abandonment of the Spanish colony of Santa Elena
at Parris Island, South Carolina (1587), the abandonment
of Tewa, Keres, and other Puebloan villages in New Mexico
(mid-16th century), and with two of the greatest human
mortality events in New World history....the cocoliztli
epidemics of 1545 and 1576 in Mexico when millions died
from hemorrhagic fevers possibly associated with a rodent
vector leveraged by extreme drought conditions (Stahle
and others, Eos, Transactions of the American Geophysical
Union, March 21, 2000).
Mike Baille describes tree ring and other evidence for
a climatic catastrophe in AD 536, which he postulates
might have been due to a comet impact with earth (Exodus
to Arthur: Catastrophic Encounters with Comets, Batsford
Ltd., London, 1999). The evidence for a 536 climate
extreme is impressive and includes record or near-record
narrow tree rings in Fennoscandia, Ireland, North America,
Chile, and Mongolia, frost rings in Mongolia, historical
evidence for dry fogs, unusual cold, crop failure, and
famine over Europe and the Middle East, and Chinese
records of dim sun, frost damage to crops and famine.
A cataclysmic volcanic eruption might also explain these
global scale climate anomalies of 536, but undisputed
ice core evidence for a major eruption at 536 or shortly
before has yet to be produced.
LaMarche (deceased) and Katie Hirschboeck describe tree-ring
evidence for a massive volcanic eruption in 1627B.C.,
which is believed by many analysts (but by no means
all) to have been the catastrophic explosion of Santorini
on the island of Thera, which may have played a role
in the collapse of the Minoan Civilization.
rings and other annually-resolved paleoclimatic proxies
such as glacial ice layers and coral bands record detailed
environmental histories and will no doubt provide many
interesting new insights into history and prehistory.
dating is an outstanding method for age determination
covering the late glacial and post-glacial period. In
any dating problem, it is always preferable to have
multiple dating methods providing concordant results.
But there is a special relationship between dendrochronology
and radiocarbon dating, because wood samples that have
been exactly dated to the calendar year by dendrochronology
have then been analyzed for radiocarbon content. It
was found early on in the development of radiocarbon
dating that carbon dates systematically deviated from
true calendar age determined from tree rings. This systematic
deviation is due to long-period variations in the solar
magnetic field that would alternately shield the earth
from incoming cosmic radiation and thereby alter the
production rate of atmospheric radiocarbon. This systematic
error in carbon dating is now well recognized and routinely
corrected. So it is entirely true to say that dendrochronology
has helped improve the accuracy of radiocarbon dating
over the last 10,000 years or so during which we have
continuous tree-ring chronologies.
dating is the most accurate and precise dating method
in geochronology, capable of dating determinations to
the season of a specific calendar year. However, the
oldest known trees on earth are just a shade less than
5000 years old, so tree-ring dating is really only possible
during the post-glacial period. Dendrochronologists
have been able to extend exact tree-ring chronologies
back to 10,000 years in rare circumstances by including
older wood found in archaeological or subfossil deposits,
but most tree-ring chronologies are less than 1000 years
are many other natural timekeepers. The horizontal sedimentary
rock strata seen in the sidewalls of the Grand Canyon
of the Colorado River have to be among the most famous
pages of earth history, recording deep geological time
with the evolutionary changes in life witnessed in the
beautiful rock strata of western North America. But
there are also many excellent natural archives that
record annual changes, and my personal favorites include
annually banded corals (the dating of which is referred
to as sclerochronology), varved sediments found at the
bottom of proglacial lakes and in a few other lacustrine
and marine settings, and annually banded ice found in
some glaciers worldwide.
my opinion, we are not doing enough to protect ancient
climate sensitive forest trees, banded coral heads of
the tropical oceans, or even the annually layered ice
found in tropical glaciers such as Quelccaya in Peru
or Kilimanjaro in East Africa. Coral reefs need more
serious protection from overfishing and water pollution.
And it may be ironic, but coral reefs in the tropical
oceans and ice sheets at the summits of tropical mountains
are both threatened by global warming, and yet many
political leaders are in denial about our wasteful overconsumption
of fossil fuels and the harmful consequences of that
waste to our environment, economy, and even national
forests with giant trees get a good deal of press, deservedly
so, but not the dwarfed, gnarly, old-growth trees found
on harsh sites throughout the United States and really
around the world. Our public vision of old growth tends
to perceive redwoods or other huge trees of temperate
rainforests, and not the stubby, stunted, and most ancient
of trees such as the bristlecone pine, the blue oaks
of the California foothills, or the craggy chestnut
oak still found along exposed positions of the Blue
Ridge Parkway. Out of ignorance for the true antiquity
of these sorts of super senescent trees, they continue
to be destroyed for very mundane reasons. You can see
an example of this type of low-stature noncommercial
woodland at our website on the Ancient Cross Timbers
in the south-central USA (http://www.uark.edu/xtimber).
I believe that if people realized that many trees in
noncommercial woodlands were old at the birth of American
democracy, then they might very well have a higher opinion
of these austere survivors and the intricate natural
histories they preserve in their centuries-long tree-ring
series. If we realized that these ancient woodlands
on stressful sites scattered throughout the United States
were indeed one part of the complex vegetation mosaic
that made up the presettlement natural landscape, then
we might think twice about scraping away these authentic
Americans for chip mills, parking lots, or new homes
landscaped with still more Bermuda grass and ornamental
pear trees. --
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