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Discussion BoardsOpen MenuDiscussion Boards Open Menu
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how do I change my email address[ Edited ]
07-04-2014 09:45 AM - last edited on 07-04-2014 09:50 AM by Russel-E
I want to change me email address so that I can access photosmart essential
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Definitions for exacerbating
This page provides all possible meanings and translations of the word exacerbating
Princeton's WordNet
aggravating, exacerbating, exasperating(adj)
making worse
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For More Information
To discuss your case with us please call us at either 713-572-4VET (4838) or toll free 877-425-4VET (4838) or complete our contact form .


text:
Aventine the new flagship?
View Single Post
December 9 2008, 05:14 AM
Lieutenant Junior Grade
Re: Aventine the new flagship?
Herbert wrote:
Sorry about that. In future I will quote my sources.
True, the
is a test bed of new technologies at the moment. But a working quantum slipstream drive in a Federation starship is a major leap in technology and capability. Like fighters moving from propeller driven propulsion to jet engines or breaking and surpasing the sound barrier. Such a leap that previous classes have been rendered obsolete if they cannot be fitted to the new drive system.
How DARE you not quote your WIKIPEDIA source and their dictionary definition of a flagship, which, if you had written yourself, would have been almost the same! This is an egregious error! Please, please, don't let that happen again.
just kidding.
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- Infowars - http://www.infowars.com -
Senate Intelligence Committee to hold hearings on Libya attack after election
Posted By kurtnimmoadmin On October 25, 2012 @ 2:36 pm In Old Infowars Posts Style,Tile,War on Terror | Comments Disabled
Greg Miller
October 25, 2:55 PM
The Senate Intelligence Committee announced plans Thursday to hold a series of hearings on the attack on U.S. diplomatic facilities in Libya. But the panel scheduled its first session on the politically charged issue for Nov. 15, a week after the presidential election.
The committee said its inquiry, starting with a session closed to the public, will focus on "the intelligence and security situation surrounding" the Sept. 11 attack in Benghazi that killed the U.S. ambassador to Libya and three other Americans.
Sen. Dianne Feinstein (D-Calif.), the chairman of the committee, recently said in a television interview that it "was possibly a mistake" for the nation's spy chief to provide talking points to lawmakers about the nature of the attack before details were clear.
Article printed from Infowars: http://www.infowars.com
URL to article: http://www.infowars.com/senate-intelligence-committee-to-hold-hearings-on-libya-attack-after-election/
Click here to print.
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New York Knicks v Los Angeles Lakers
Carmelo Anthony of the New York Knicks steps onto the floor to take... News PhotoBasketball - Sport,California,Carmelo Anthony,City Of Los Angeles,Horizontal,Los Angeles Lakers,NBA Pro Basketball,New York Knicks,One Person,People,Sport,Staples Center,USAPhotographer Noah GrahamCollection: National Basketball Association 2012 NBAELOS ANGELES, CA - DECEMBER 25: Carmelo Anthony #7 of the New York Knicks steps onto the floor to take on the Los Angeles Lakers at Staples Center on December 25, 2012 in Los Angeles, California. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and/or using this Photograph, user is consenting to the terms and conditions of the Getty Images License Agreement. Mandatory Copyright Notice: Copyright 2012 NBAE (Photo by Noah Graham/NBAE via Getty Images)


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The Wound Care Clinic is located at: 2355 Union Rd. Cheektowaga, NY 14227.
Health Care Studies Professor Justine Tutuska, accompanied by her students, traveled to the Dominican Republic to work on Public Health Issues and school building.
©2014 Daemen College : 4380 Main Street, Amherst, NY 14226 : 800.462.7652


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A Chicago pizza delivery man died Jan. 13 after he was shot and robbed one day before.
According to The Chicago Tribune, Charles Gordon, a pizza cook at Arnie's Pizza, had just finished his shift on Jan. 12, when he agreed to deliver a few pizzas as a favor for his boss. When he arrived at the location he determined the apartment building was vacant and turned to leave. Immediately, two men emerged from behind the building and attacked him.
The pair punched Gordon, threw him to the ground and, after robbing him of $10, one man shot him in the chest with a .380 caliber handgun.
Gordon then drove himself several blocks to a Burger King where his wife, Roschelle, worked, and told her he'd been shot. He then gave police descriptions of the men, and was taken to the hospital.
Following a foot chase, police arrested the two men and recovered the weapon suspected of killing Gordon. Adam Titus, 17, of Markham, was charged with first-degree murder and armed robbery in connection with the death. Charges are pending against a second man.
Gordon, who worked nearly every day as a pizza cook to support his seven children, died the next morning during surgery.
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North Georgia History Begins at the Cherokee County History Museum and Visitors Center.
The Museum is located at 100 North Street, Suite 140
Canton, GA 30114
Get Directions
It is open Wednesday – Friday 10:00 a.m. – 5:00 p.m. and Saturday 10:00 a.m. – 3:00 p.m.
Admission is free.
The Cherokee County History Museum and Visitors Center is operated by the Cherokee County Historical Society with support from the Cherokee County Board of Commissioners. The Historical Society relies on the support of volunteers to staff the Museum; if you are interested in volunteering in the Museum, please contact the Executive Director, Stefanie Joyner, at firstname.lastname@example.com. The small Museum features permanent exhibits about Cherokee County history, including interactive iPad presentations. In addition, the Historical Society staff presents temporary exhibits on different subjects 3 to 4 times a year.
The Museum also features these short videos about real Cherokee County citizens who have a story to tell. The Historical Society received an Archival Excellence Award from the Georgia Archives for this exhibit.
Cherokee County's history is filled with rich figures; all with a tale to tell. The following people would like to guide you through the county they knew – the places and people and the events that shaped their lives…click their image to watch the videos.
Little Fourkiller
1838, age 10 years
Shoal Creek
He and his family were forcibly removed along
with over 14,000 Native Americans to Oklahoma
on the Trail of Tears.
Clara Bedell
1902, Age 18
She was the daughter of the sheriff of Canton
and attended Reinhardt Normal School.
Magnolia Thomas
1930, Age 40
She was a graduate of Spelman Seminary
in Atlanta and she taught school in Woodstock.
New Temporary Exhibit
Digging for Treasure
A History of Mining in Cherokee County
May 20 – September 26, 2015
The exhibit explores the many types of mining which have occurred in the county, beginning with the Gold Rush of 1829. Maps showing the various mines and geology of the area are on display, as well as historic photographs of the mines. Artifacts from the Pascoe, Creighton, Franklin and Cherokee gold mines are highlighted, and specimens of several locally mined minerals are included.
Cherokee County History Museum
100 North Street, Ste. 140; Canton, 30114
Wednesday—Friday 10:00am—5:00pm
Saturday 10:00am—3:00pm
Free Admission


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Plant circadian systems regulate various biological processes in harmony with daily environmental changes. In Arabidopsis thaliana, the underlying clock mechanism is comprised of multiple integrated transcriptional feedbacks, which collectively lead to global patterns of rhythmic gene expression. The transcriptional networks are essential within the clock itself and in its output pathway.
Here, to expand understanding of transcriptional networks within and associated to the clock, we performed both an in silico analysis of transcript rhythmicity of transcription factor genes, and a pilot assessment of functional phenomics on the MYB, bHLH, and bZIP families. In our in silico analysis, we defined which members of these families express a circadian waveform of transcript abundance. Up to 20% of these families were over-represented as clock-controlled genes. To detect members that contribute to proper oscillator function, we systematically measured rhythmic growth via an imaging system in hundreds of misexpression lines targeting members of the transcription-factor families. Three transcription factors were found that conferred aberrant circadian rhythms when misexpressed: MYB3R2, bHLH69, and bHLH92.
Transcript abundance of many transcription factors in Arabidopsis oscillates in a circadian manner. Further, a developed pipeline assessed phenotypic contribution of a panel of transcriptional regulators in the circadian system.
The Arabidopsis thaliana (Arabidopsis) circadian clock drives growth and development in response to daily and seasonal change [1]. This is of ecological relevance as the clock has been shown to be critical for plant fitness and appears to be evolving in correlation with latitude [2,3]. In Arabidopsis, the clock system is proposed to be composed of integrated transcriptional feedbacks [4-6]. These loops drive global gene expression rhythms [7]. In fact, estimates of the total global consortium of cycling genes has ranged from 2% to 36% of all Arabidopsis transcripts [8-10]. These global regulatory patterns of transcript abundance demonstrate that whole regulatory and metabolic pathways are under clock control [8,10,11]. This exquisitely coordinated regulation is thought to be the purpose of the clock. Overall one can see an emerging, systems-level understanding of the complicated biological mechanisms composed of transcriptional networks driven by the clock. Functional tests of these hypotheses are required to fully expand the integrated network.
Understanding the molecular nature of the circadian oscillator is an ongoing task. Within the currently understood core of the oscillator are the sequence related MYB-like factors CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY). These genes were first discovered through misexpression studies, as overexpression of either was found to generate an arrhythmic clock [12,13]. Further work on these factors [14], and the identification and characterization of other clock genes [15], resulted in an elegant description of the rhythm-driving oscillator [16-18]. Here a four-loop model has been proposed where in the core of this oscillator lies CCA1/LHY and the pseudo-response regulator TIMING OF CHLOROPHYLL A/B-BINDING PROTEIN (CAB2, also termed LHCB1*1) GENE EXPRESSION 1 (TOC1) [16-18]. This core was confirmed as the cca1 lhy toc1 triple mutant has seriously attenuated rhythmic behavior [19]. CCA1/LHY are genetically transcriptional repressors of TOC1, and TOC1 is a positive genetic factor, with an as of yet unproven biochemical function [20], that functions in transcriptional induction of CCA1 and LHY. The CCA1/LHY loop is further regulated by a morning loop that contains the TOC1 sequence-related genes PSEUDORESPONSE REGURATOR 9 (PRR9) and PRR7. In turn, the TOC1 arm of the clock is also regulated by a loop that includes the GIGANTEA (GI) flowering-time gene [15,17]. Current models infer as of yet unidentified transcription factors in this looped network [16].
Circadian-regulated transcription factors should confer the complete array of phased rhythms of transcript accumulation that is observed [8,10]. As for example, the MYB-like transcription factors CCA1 and LHY, thought core for normal clock function, are predicted to drive output regulation [10,8]. Additionally, the MYB-transcription factor EARLY PHYTOCHROME RESPONSIVE 1 (EPR1), the MADS-domain factor FLOWERING LOCUS C (FLC), and a GARP transcription factor, LUX ARRYTHMO (LUX), were also reported to be involved in circadian system [21-23]. These three genes could additionally control a suite of transcript outputs from the clock. Another example of the regulation of circadian outputs by clock-controlled transcription factors is the regulation of the anthocyanin biosynthesis pathway, where structural enzymes for this secondary metabolite are encoded by genes coordinately regulated by a cycling output transcription factor called as PRODUCTION OF ANTHOCYANIN PIGMENT 1, PAP1 [10]. Thus, not all rhythmic transcription factors feedback to the oscillator. We believe it is likely that a small set of transcription factors await to be discovered that can modulate clock function, and just as importantly, we expect that a large set of transcription factors are themselves regulated at the transcript accumulation level to drive the physiological suite of rhythmic outputs.
For the circadian clock to drive rhythmic expression of such a large part of the genome, and for these genes to be phased at all times of the subjective day (no phase bias exists, as shown by [10]), a suite of transcription factors must be implicated in the clock-output system. The Arabidopsis genome encodes more than 1500 transcription factors that belong to more than 30 different families [24]. Each family of transcription factors was characterized based on the definition of containing a highly conserved DNA-binding domain(s). For example, the Arabidopsis genome contains 133 members of MYB transcription factor superfamily, 162 genes encoding basic helix-loop-helix (bHLH) transcription factors, 75 distinct members of basic region/leucine zipper motif (bZIP) transcription factors [25-27]. Some transcription factors were reported as activators and repressors to compose complexly integrated regulatory loops in the plant circadian system [17]. However, functional characterization of the vast majority of Arabidopsis transcription factors still remains.
Here, we took two overlapping genomic approaches to further catalog the repertoire of transcription factor use within the oscillator and in expression of output traits. These companion approaches identify previously uncharacterized plant genes involved in the circadian system, and further dissects this complex signaling network. For this, we surveyed existing microarray results of the MYB, bHLH, and bZIP transcription factor families and determined those that are clock regulated, and separately, systematically analyzed circadian rhythms in misexpression mutants targeting transcription factors, via time-lapse imaging. We report the discovery of three misexpression lines that have altered circadian parameters. Our suite of analyses lead us to conclude that although many transcription factors do not contribute to normal clock function, transcription factors previously non-described within the clock can be discovered through systematic tests, including computational surveys.
Defining circadian expression within transcription-factor families
As suites of transcripts are clock regulated in Arabidopsis [10], we hypothesized that this was due to rhythmic accumulation of transcription factors. MYB, bHLH, and bZIP are the predominant factor families previously implicated in light- and clock-regulated accumulation of targets [25-27], so we decided to test how prevalent individual rhythms are within these families. Previously, we have collectively reported no less than 368 genes predicted to encode transcription factors in the MYB (131), bHLH (162), and bZIP (75) transcription factor families in Arabidopsis [25-27]; these were the target pools queried. We accessed expression profiles of circadian experiments, NASCArrays Experiment Reference Number: NASCARRAYS-108 [28], using Affymetrix ATH1 arrays containing 22,746 probe sets in the public microarray database, GENEVESTIGATOR [28,29]. This probe set represents 122 MYB, 111 bHLH, and 67 bZIP genes. In these circadian datasets, a total of 185 of these genes (51 MYB, 81 bHLH, and 53 bZIP) were found to be expressed at least one time point (at p < 0.06). We noticed that the expression of more than half of MYB genes was below detection level on these hybridization samples. Perhaps this implies that many MYB transcripts in this experimental protocol were tissue or growth-stage specific. In contrast, most bHLH and bZIP genes were detected in this array experiment. The expression data we processed from these 185 genes was sufficient for further in silico analyses.
We scored the expression values of the 185 genes using the modified Cosinor analysis [30]. This analysis was used successfully in previous experiments to score the circadian expression for genes in Drosophila, mouse, and Arabidopsis [10,31,28]. A previous study using this approach in Arabidopsis employed three threshold scales of significance to assess probability (pMMC-β): < 0.02, 0.05, and 0.1 [28]. We used the same confidence cut-offs to define rhythmic genes (Table 1). A total of 42 transcription factor genes with a pMMC-β value of 0.05, which reflected 9 MYB, 19 bHLH, and 14 bZIP transcription factors, were scored as rhythmic (Table 1, Additional file 1). The percentages of rhythmic bHLH and bZIP genes within each respective family were similar to, or even slightly higher than, that of the set of "all" genes (Table 1). The percentage of rhythmic MYB genes was less than that of the bHLH, bZIP, and the set of all genes; however, noteworthy is the percentage of expressed-MYB on the array that was, itself, lower than other sets. A graphic representation of the expression patterns illustrates when a given peak occurred during transcription factor oscillation (in (h) hours relative to zeitgeber time, which is the time of the last external temporal cue such as the dawn signal of lights-on), (Figure 1). It was noted that many bZIP genes were transcribed during the photophase of the day, whereas many MYB genes peaked during the skotophase (Figure 1). These collective results highlight that, as expected, many transcription factors oscillate, and do so at many discreet phases of the daily cycle.
Table 1. MYB, bHLH and bZIP genes scored as rhythmic by COSOPT. Total number of MYB, bHLH and bZIP on the genome, array and expressed in the circadian experiment (p < 0.1) is shown. Number of rhythmic genes is represented as total and percentage of the transcription factors on the array. The rhythmic scoring was performed at three different pMMC-beta thresholds.
Figure 1. Oscillation peaks of various transcription factors. Transcripts from 42 transcription factors oscillate with certain peaks in circadian phase of one subjective diurnal day. The peak expression of these genes is illustrated (ZT h). MYB, bHLH, and bZIP transcription factors are colored in pink, yellow, and blue, respectively. Each trace illustrating the cycling patterns of expression is shown on the right side of this figure.
Additional file 1. Supplemental Table 1 – Circadian regulated MYB, bHLH and bZIP genes. Circadian expression values available in the public database GENEVESTIGATOR were scored for circadian regulation using the modified cosinor analysis program COSOPT. Mean of expression levels, period length, phase values (ZT) and pMMC-β are represented. COSOPT (pMMC-β < 0.05) without linear regression are listed here.
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Circadian function of transcription factors
In the in silico analysis (Figure 1), we found several night-expressed transcription factors. We wondered if such transcription factors could be the as of yet unidentified clock components proposed by current models [16,17]. To generically test this hypothesis, we surveyed T-DNA tagged lines targeting these transcription factors, obtained from the public stock center, and measured circadian rhythms of these mutants in our system. However, no strong alternations of the circadian rhythm were observed in any of these lines under our assay condition (data not shown). Our observation suggested that none of these factors are the predicted clock element, and thus alternative approaches must be used to define such circadian mutants.
To identify transcription factors that function within the circadian system, as part of control or slave oscillator, we assessed rhythmic output of lines targeted to misexpress a given transcription factor. These experiments, because they are overexpression studies, allowed us to circumvent genetic redundancy. We feel that this is a particularly important consideration in the Arabidopsis clock, as the Mybrelated sequences CCA1 and LHY have strong circadian defects when overexpressed [12,13], but have only mild phenotypes as single gene loss-of-function alleles because of the redundancy inherent in the system, [14]. Our hypothesis was that members of the MYB, bHLH, and bZIP families are as of yet uncharacterized components of the oscillator and/or are slave components. As a test of this hypothesis, we made use of 198 plants over-expressing 39 MYB genes, 29 bHLH genes, and 4 bZIP genes (Table 2, Additional file 2). Overexpression of each transcription factor was confirmed by RT-PCR on rosette leaf cDNA from T1 plants (data not shown, e.g. Additional file 5).
Table 2. Plants targeting transcription factors analyzed in this study. A total of 198 mutants, including 198 misexpression were analyzed in this study. The misexpression lines targeted 39 MYB, 29 bHLH and 4 bZIP genes. The period phenotypes of 19 out of 22 over-expression lines were not consistent in multiple transgenics. Aberrant clock precision was only observed in 1 out of 3 bZIP48-ox lines. Numbers in ( ) indicated lines over-expressing same genes, which support that the phenotypes were caused by misexpression of the genes.
Additional file 2. Supplemental Table 2 – Estimated period length of transgenic lines overexpressing MYB transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.
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We constructed a high-throughput time-lapse imaging system, similar to one previously reported [32]. With this system, we measured the circadian rhythms of leaf movement in each transgenic line. For this, the seedlings were entrained under 24-hour light-dark cycles for ~10 days, and then the leaf positions of individual plants were imaged under constant light (LL) for an additional 7 days. The circadian parameters of the change in leaf position were analyzed (Additional files 2, 3, 4, 5 &6). We found 33 misexpressors with circadian phenotypes, and 13 out of 33 misexpressor lines targeted the three genes MYB3R2, bHLH69, and bHLH92 (Figure 2; Tables 2 and 3). MYB3R2-ox and bHLH69-ox displayed a 4–8 hour delayed phase of leaf movement rhythms (Figure 2A and 2C). Statistical analysis indicated no significant differences in circadian periodicity (Table 3; Additional files 2 and 3), while the phase difference was significant (Figure 2A–D). bHLH92-ox plants exhibited a 0.5~2 hour lengthened periodicity phenotype, compared to the wild type (Figure 2E). The periodicity differences were statistically significant; WT, 24.16 ± 0.45; bHLH92-ox line A, 26.32 ± 0.77; line B, 25.09 ± 0.32; line D, 25.16 ± 0.36; line E, 24.76 ± 0.41 (p < 0.05, R. A. E. < 0.4) (Table 3). Thus, MYB3R2 and bHLH69 controls circadian phase, and bHLH92 contributes to the regulation of circadian periodicity.
Table 3. Misexpression lines exhibit aberrant clock phenotype. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.
Figure 2. Representative leaf movement data for lines expressing a clock phenotype. Leaf movement rhythms were assayed under constant light for approx. 1 week (n = 14–28). (A, C, E) Representative traces of rhythmic leaf movement of wild-type (blue circles) and ox-lines (other colored symbols) are shown. (B, D) The phase angles normalized to a 24-h cycle (CT phase) are plotted with relative amplitude errors (RAE), which indicate the robustness of the rhythm (the lower the RAE the more robust the rhythm). The center of the circle represents a high RAE (= 1). (A, B) MYB3R2-ox, (C, D) bHLH69, and (E) bHLH92.
Additional file 3. Supplemental Table 3 – Estimated period length of transgenic lines overexpressing bHLH transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.
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Additional file 4. Supplemental Table 4 – Estimated period length of transgenic lines overexpressing bZIP transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.
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Additional file 5. Figure S1 – Confirmation of over-expression of MYB3R2 and bHLH69. Replicate seedlings from wild-type plants maintained under constant light were harvested every 4 hours. Total RNA was the substrate for RT-PCR of the coding regions of the transcription factors MYB3R2, bHLH69 and bHLH92. Results are presented as proportional to the average value after normalization with respect to TUB. (A) MYB3R2 and (B) bHLH69.
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Additional file 6. Figure S2 – Confirmation of transcriptional clock phenotype of bHLH92-ox in constant light and in constant dark. Seedlings harboring CCA1:LUC reporter genes were monitored for 4–5 days both under LL (A) or in DD (B). Representative traces of rhythmic expression of ox-plants (pink squares) and wild-type (blue circles) are shown. (C) Relative Amplitude Error (R. A. E.) calculated from the data under LL was plotted against period (h). bHLH92-ox exhibited a slightly long periodicity phenotype.
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During imaging experiments, we identified additional phenotypically altered lines in the pools of misexpression transgenics (Table 2, Additional files 2, 3 &4). A total of 19 over-expression lines exhibited aberrant periodicity, and one of the bZIPox plants (bZIP48-ox) lacked clock precision. However, phenotypes from these lines were not confirmed by other lines targeting the over-expression of the same gene. One plausible explanation is that these detected phenotypes are not correlated to the targeted gene, and perhaps was caused by coincident mutations occurring during TDNA transformation.
MYB3R2, bHLH69, and bHLH92 could influence circadian rhythms
To confirm the clock phenotypes observed in MYB3R2, bHLH69, and bHLH92 over-expression lines, we employed the promoter:LUCIFERASE (LUC) system as an assay that here is used to detect rhythmic patterns of gene expression [33]. LUC fusions to the well-characterized circadian-regulated promoter, CCA1 and to the COLD-AND CIRCADIAN-REGULATED 2 (CCR2, also termed AtGRP7) promoter were separately introduced into these MYB3R2-ox, bHLH69-ox and bHLH92-ox plants via fertilization. If these transcription factors act upstream of the clock, both CCA1 and CCR2 oscillations would be altered. Alternatively, if these genes act downstream, controlling the output pathway that regulates leaf movement, the CCA1 rhythm intimately associated with clock function would not be affected. MYB3R2-ox exhibited delayed phase phenotypes of both CCA1 and CCR2 rhythms under LL (Figure 3A and 3B). bHLH69-ox also delayed the phase of the CCA1 and CCR2 rhythm (Figure 3C and 3D). In our mathematical analysis, we could not find any significant effects on the circadian periodicity (Additional file 7). The second peak positions of CCA1 rhythms in control, MYB3R2-ox, bHLH69-ox and bHLH92-ox were 51.44 ± 0.44, 54.22 ± 0.71, 53.18 ± 0.45 and 53.49 ± 1.03, respectively (Additional file 7). P-values for MYB3R2-ox and bHLH69-ox were less than 0.01, while p-value for bHLH92-ox was 0.07. In the second peaks of CCR2 rhythm, the values in control, MYB3R2-ox and bHLH69-ox were 37.01 ± 0.23, 39.37 ± 0.63 and 39.81 ± 0.89 (p < 0.01). Thus, we found that overexpression of MYB3R2 and bHLH69 altered both CCA1 and CCR2 rhythms, suggesting that these genes can control core-clock functions, rather than being specific to the leaf-movement-output pathway.
Figure 3. Confirmation of transcriptional clock phenotypes under constant light. Seedlings harboring CCA1:LUC or CCR2:LUC reporter genes were monitored under constant light for 4–5 days. Representative traces of rhythmic expression of ox-plants (pink squares) and wild-type (blue circles) are shown. (A, B) MYB3R2-ox, (C, D) bHLH69-ox. (A, C) CCA1:LUC, (B, D) CCR2:LUC.
Additional file 7. Figure S3 – Estimated period and phase of MYB3R2-ox, bHLH69-ox and bHLH92-ox. Estimated period length and phase values were calculated by BRASS. (A) Estimated period of CCA1 rhythm. (B) Peak positions of second peak in CCA1 rhythm. (C) Estimated period of CCR2 rhythm. (D) Peak positions of second peak in CCR2 rhythm. Data are presented as mean ± S.E. with n of 12–24 plants. * P = 0.01. No significant difference in periodicity was observed (A and C).
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We measured CCA1 and CCR2 rhythms in MYB3R2-ox, bHLH69-ox, and bHLH92-ox plants in constant darkness (DD) (Figure 4). This allows us to compare their behavior to the LL phenotypes. Interestingly, MYB3R2-ox plants had an advanced phased CCR2 rhythm and a delayed phase of CCA1 expression in DD (Figure 4A and 4B). The peak positions in control and MYB3R2-ox were 37.44 ± 0.44 and 35.86 ± 0.46 in the CCR2 rhythm, and 51.23 ± 0.61 and 53.92 ± 0.69 in the CCA1 rhythm (p < 0.01) (Additional file 7). A delayed phase of CCA1 in DD was seen in bHLH69-ox lines (Control = 51.23 ± 0.61, bHLH69-ox = 54.05 ± 0.34; p < 0.01) (Figure 4C and 4D, Additional file 7). bHLH92-ox plants also exhibited a clock phenotype in DD. Here an effect on CCA1 phase was detected (Additional file 6). The values were 53.94 ± 0.57 (p < 0.01). We thus concluded that misexpression of any of these three transcription factors could alter clock parameters. However, the specific nature of the phenotypic effects depended on the light conditions and the output measured.
Figure 4. Confirmation of transcriptional clock phenotypes in constant dark. Seedlings harboring CCA1:LUC or CCR2:LUC reporter genes were monitored in constant darkness for 4–5 days. Representative traces of rhythmic expression of oxplants (pink squares) and wild-type (blue circles) are shown. (A, B) MYB3R2-ox, (C, D) bHLH69-ox. (A, C) CCA1:LUC, (B, D) CCR2:LUC.
MYB3R2 and bHLH69 could alter clock-gene expression
As described above, we detected circadian alternations when misexpressing given transcription factors. Because the effect of bHLH92-ox was dependent on the light condition, we continued our focus on MYB3R2-ox and bHLH69-ox to further characterize the molecular basis for their phenotypes. To this end, we analyzed transcripts of the central oscillator genes CCA1, LHY, TOC1, and GI in MYB3R2-ox and bHLH69-ox (Figure 5). The MYB3R2-ox and bHLH69-ox plants were entrained under 12 hour light/12 hour dark cycles, and then transferred to constant light conditions. Replicate samples from these plants were harvested every 4 hours for RNA isolation and expression analysis using reverse transcriptase (RT)-PCR. Both MYB3R2-ox and bHLH69-ox were found to result in a repressed transcript level of LHY and TOC1 (Figures 5A and 5B). CCA1 mRNA was found to be slightly decreased in MYB3R2-ox, while this was increased in bHLH69-ox (Figure 5C). Interestingly, in bHLH69-ox, induction of GI expression was found to display a nearly opposite phase with high expression level (Figure 5D). At zeitgeber time (zt) = 4, LHY and CCA1 mRNA are highly accumulated in MYB3R2-ox. This response could be accounted for by an acute light response. Alternatively, a phase delay or defective entrainment in MYB3R2-ox might cause the high expression seen at this time-point. Interestingly, the alteration in GI expression did not result in a dramatic alteration in the timing of the floral induction (Additional file 8). We suggested that MYB3R2 functions as a regulator of CCA1, LHY, or TOC1 transcription, and suggest that bHLH69 plays a similarly important role to regulate CCA1 and GI expression.
Figure 5. Clock-gene expression profiles in selected transcription factor misexpression lines. Seedlings from ox- and wild-type plants were harvested every 4 hours. Total RNA was the substrate for RT-PCR against the coding regions of the core-clock genes CCA1, LHY, GI, or TOC1, and as a control, TUBULIN (TUB). Results are presented as proportional to the average value after normalization with respect to TUB. Expression profiles in the control, MYB3R2-ox and bHLH69-ox were represented as blue, orange, and pink lines, respectively. (A) LHY, (B) TOC1, (C) CCA1, and (D) GI.
Additional file 8. Figure S4 – The effects of over-expression of MYB3R2 and bHLH69. Flowering time of MYB3R2-ox and bHLH69-ox plants was measured under long day. Leaf number at flowering time were plotted against the genotype and line tested. Data are presented as mean ± S.E. with n of 9–14 plants. * P = 0.038. No significant differences were detected in the flowering time of other lines.
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We next investigated whether the MYB3R2, bHLH69, and bHLH92 genes were transcribed in a circadian manner. We performed this experiment as the expression profiles of these three genes were not part of the publicly available datasets described above [29]. The mRNA accumulation patterns of these transcription factors were assayed by RT-PCR from RNA extracted from plants grown under light-dark cycles and then transferred to LL (Figure 6). MYB3R2 and bHLH92 were likely to be expressed in a circadian manner with a peak between late night and dawn. In contrast, the mRNA accumulation of bHLH69 was not found to oscillate.
Figure 6. Transcript accumulation of transcription factors that have clockcontrolling properties. Replicate seedlings from wild-type plants maintained under constant light were harvested every 4 hours. Total RNA was the substrate for RT-PCR of the coding regions of the transcription factors MYB3R2, and bHLH69 and bHLH92. Results are presented as proportional to the average value after normalization with respect to TUB. (A) MYB3R2, (B) bHLH69, and (C) bHLH92.
Identification of previously functionally uncharacterized transcription factors in the clock
Here we describe that MYB3R2, bHLH69, and bHLH92 can contribute to the plant-circadian system. Alongside the characterized transcription factors CCA1, LHY, EPR1, FLC, and LUX, this now adds to the list of transcriptional clock-modulators. We provide evidence that MYB3R2 and bHLH69 influences circadian phase, whereas bHLH92 influences phase and periodicity, dependent on environmental conditions. These effects were not as strong as misexpression of CCA1, LHY, or LUX. It is still unclear how MYB3R2, bHLH69, and bHLH92 function in the clock system. Their specific effects depended on the light environment and on the output measured. Expression analysis showed that misexpression of MYB3R2 and bHLH69 resulted in altered transcript levels of clock genes; however, the circadian rhythms still kept 24-h periodicity albeit with aberrant phase. Thus, transcription factors identified here may play a role in environmental input to the clock or the mediation of its effects, rather than functioning as central-clock components. Such processes are described [34]. A future effort to explore a detailed analysis of these transcription factors and the identification of target DNA elements remains to be carried out.
Plant circadian systems possess interlocked feedback loops [17-19]. In addition, there are various junctures for signal convergence and divergence in the input and output pathways [35,36]. Though a set of clock regulating transcription factors has already been identified sufficient to describe much of the oscillator framework [11], many other components are believed to still be lacking [10].
We described an additional 20 misexpression lines that exhibit altered clock phenotypes. Whether these genes are components of input core, or output pathways, is as of yet unknown. This was as the phenotypes detected in these lines were not substantiated with alternative transgenic inserts. Forward-genetic analysis of loss-of-function phenotypes of these lines is worth further attention to determine their function in the clock.
A current mathematical model proposes the existence of an unknown transcription factor "X", which activates CCA1 and LHY [16,17]. We tested hundreds of transcription factors as a pilot study of functional phenomics within the circadian clock, but it appears that in this test, we did not find "factor X." Still, functional analysis of more than a thousand transcription factors still remains. Thus, our pilot efforts substantiate that it is worthwhile to interrogate, via further functional genomic efforts, all known Arabidopsis transcription factors and to analyze their circadian responses.
Transcriptional networks in clock output pathways
To dissect the transcriptional regulation in circadian clock, we employed a systematic analysis of global-gene expression. In our in silico analysis, up to 20% of the transcription factors assayed were clock controlled (Table 1). This percentage is slightly higher than that of "all" circadian rhythmic genes in the Arabidopsis genome [10]. This implies that the circadian oscillations in the output pathways are not just regulated by a select group of transcriptional feedbacks, and actually require a large number of rhythmic transcription factors. In contrast, the mammalian system is reported to only use around 16 cycling transcription factors, which oscillate in a circadian manner, to regulate the clock [37]. The evolution of transcription-factor function and recruitment of molecular targets leads to the linking of many processes of plant physiology to the circadian system. Some components of these systems direct the clock itself, whereas others are only components of rhythmic physiological outputs.
Here we employed two genomic approaches as a pilot study to explore clock function. We found three genes that could modulate circadian parameters. Many other transcription factors oscillated in a circadian manner. This implicates these factors in physiological circadian outputs. Further exploration, with our described approaches, should bring novel insights in circadian input and output pathways, and identify genes previously non-described as functional within the central-clock oscillator.
Expression analyses through the public database
Gene codes of all transcription factors studied, referred to as AGI numbers, were collected within the Arabidopsis Information Resource. The gene-expression profiles in circadian experiment were available from the public microarray database Genevestigator [29,28]. Expression values from the data were subject to score circadian rhythms with COSOPT [30]. Data was collated without non-linear regression (Additional file 1).
Generation of transcription factor OX-lines
Full-length coding sequences (ATG-to-Stop) from MYB-, bHLH- and bZIP transcription factors were amplified from respective cDNAs by PCR using attB-sites containing gene-specific primers. Gateway Entry clones were generated via BP-reaction using the vector pDONR201 (Gateway system, Invitrogen, USA). Via LR-reaction the transcription factor cDNAs were transferred behind the double enhancer cauliflower mosaic virus 35S promoter in the plant expression vector pLEELA [38]. Transgenic lines were generated by Agrobacterium tumefaciens-mediated transformation of Arabidopsis plants (Col-0) according to the floral-dip protocol [39]. All transgenic lines were selected and self-fertilized. T2 plants were analyzed in this study.
Plant growth conditions
Seeds were surface sterilized with a 70% ethanol rinse, immediately followed by a rinse with 33% bleach, and then a twice washed with sterile water. The seeds were then aseptically sown on Murashige-Skoog (MS) 1.5% agar medium containing 3% sucrose (pH 5.7) with suitable antibiotic (25 mg/L Kanamycin or 12 mg/L Phosphinotrycin; dependent on the transgene) followed by stratification at 4°C for 4–5 days. Seedlings were grown for 9 days at 22°C under 12 hr light/12 hr dark cycles of 75 μmol m-2 sec-1 cool white fluorescent light. For flowering-time measurements, experiments were as described [40].
Leaf movement measurement
After 9 days of entraining growth, seedlings were transferred to fresh MS 1.5% agar medium (pH 5.7) containing 3% sucrose without antibiotic, and then agar blocks harboring single seedlings were placed to 25-well square tissue culture dishes (Bibby Sterilin). A set of twenty seedlings in a set within each dish were viewed from the side from plates in a vertically placement. The seedlings were entrained as described above for another day, and then were placed into a growth chamber for imaging over 1 week under constant white light (25–40 μmol m-2 sec-1) at a constant 22°C. A total of 14 dishes, containing 280 seedlings, were prepared and imaged with 14 monochromatic charge coupled device video cameras per an experiment. Images of seedlings from every camera were transferred to a computer via a Flashbus card and through a custom-built parallel-port controller unit (Universal Imaging, Germany) (system development by Visitron Systems), and were captured and saved every 30-min with a computer program Metaview 4.5 (Universal Imaging) over a week. The vertical positions of primary leaves from the images were measured and analyzed using Metamorph and BRASS, the latter provided by Prof. Andrew Millar (University Edinburgh) as described [28,32]. Period lengths were estimated from the leaf movement data by the fast Fourier transform nonlinear least-squares method [41]. Mean period estimates for each line were based on 10–20 leaf traces from two to four independent experiments analyzed.
Luciferase imaging
Imaging was performed as described via established protocols, where the light was provided from red and blue light-emitting diodes at ~2 μmol m-2 s-1 [5,42,43]. Period length and Relative Amplitude of error (RAE) were estimated using FFT-NLLS program [41].
RNA isolation and reverse transcriptase-PCR
Seedlings grown for 1 week under LD cycles and replicate samples were harvested every 4 hours under LL conditions. Total RNA was isolated from the seedlings using the RNeasy Plant Mini Kit (Qiagen, USA), and then was treated with DNase I before reverse transcription. Reverse transcription was performed on 1.0 μg of total RNA with SuperscriptII (Invitrogen). Quantitative PCR were performed with iQ5 real-time PCR system (BIO-RAD). Gene-specific primers were described previously: CCA1, LHY and TOC1 [44], GI and TUB [15]. Primers for MYB3R2, bHLH69 and bHLH92 were designed as follow:
bHLH69-FW, 5'-CCATCCTAATGACGCTCTCTTC-3'
bHLH69-RV, 5'-ATCAGTGGCTTGACCTCTCCTA-3'
bHLH92-FW, 5'-CTGAGAAAGAATTGGGAGGAGA-3'
bHLH92-RV, 5'-GACCATCCTTTGCTGATTTTTC-3'
Authors' contributions
SH, RS, MJ, BW, and SJD conceived the experiments. SH, MAD, and SJD wrote the paper. RS, MJ, TM, and BW generated and confirmed overexpression and insertion lines. SH carried out the circadian experiments. SH and MAD carried out the in silico analyses.
We are most grateful for editorial comments from Dr. Chiarina Darrah. Additional gratitude is extended to Mr. Wolfgang Feneberg (Visitron Systems GmbH, Germany) for helping to develop the leaf-imaging system, and to Dr. Kieron Edwards, Dr. Paul Brown, and Prof. Andrew Millar (University of Edinburgh, UK) for advising our data analysis. We thank Nottingham Arabidopsis Stock Centre for providing seeds. This work was supported in the SJD group by a grant from the Deutsche Forschungsgemeinschaft (DA1061/2-1), which financially supported MAD, from the German-Israeli Project Cooperation (DIP project H 3.1), and from the Max Planck Society. Work in the BW group acknowledges funding from the German Federal Ministry of Education and Research (BMBF) in the context of the German plant genomics program GABI (Förderkennzeichen 0312273).
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Divisional Playoffs - Houston Texans v Baltimore Ravens
Haruki Nakamura, LaQuan Williams and Morgan Cox of the Baltimore... NyhetsfotoAFC Divisional Playoff Game,Amerikansk fotboll,Baltimore - Maryland,Baltimore Ravens,Firande,Haruki Nakamura,Horisontell,Idrottsarena,LaQuan Williams,M&T Bank Stadium,Maryland - Delstat,Match - Sport,Morgan Cox - Amerikansk fotbollsspelare,NFL,Playoffs,Sport,USAPhotographer Patrick McDermottCollection: Getty Images Sport 2012 Getty ImagesBALTIMORE, MD - JANUARY 15: Haruki Nakamura #43, LaQuan Williams #15 and Morgan Cox #46 of the Baltimore Ravens celebrate on the one-yard line during the second quarter of the AFC Divisional playoff game at M&T Bank Stadium on January 15, 2012 in Baltimore, Maryland. Baltimore won 20-13 in regulation. (Photo by Patrick McDermott/Getty Images)


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Paradox is a 2009 British science fiction police drama, starring Tamzin Outhwaite as Detective Inspector Rebecca Flint. Written by Lizzie Mickery and produced by Clerkenwell Films for the BBC, it was filmed and set in Manchester, England.
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National Cattlemen's Beef Assn
National Cattlemen's Beef Association or NCBA, an advocacy group for beef producers in the United States, reports that it works "to increase profit opportunities for cattle and beef producers by enhancing the business climate and building consumer demand."
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- corporate name changes and mergers may cause figures to differ from those of the Center for Responsive Politics.
- organization totals include known subsidiaries of the organization.
For more information, please see our campaign finance methodology page. Lobbyist bundling data is described on our lobbyist bundling methodology page.
Latest FEC Datacovers through committee's December 31, 2012 filing.
Summaryoverview of the committee's finances
- Total Raised:
- $1,051,497
- Total Spent:
- $838,679
- Cash on Hand:
- $393,155
- Debts:
- $0
Standardized Donation Informationfederal data covers roughly through Q2 2014
state-level data is not available in bulk for 2014. for current state data, browse http://followthemoney.org/
Top Recipients
- PAC Color Block
- Organization's PAC(s)
- Employee Color Block
- Associated Individuals
Republicans vs. Democratsin dollars. "Other" includes 3rd parties and organizations without official party affiliation.
State vs. Federalin dollars
Top PAC Recipients
- PAC Color Block
- Organization's PAC(s)
- Employee Color Block
- Associated Individuals
Lobbying$656,187 Spent
Figures are based on lobbying activity reported to the Senate Office of Public Records. Reported dollar amounts are required to be accurate only to the nearest $20,000. For organizations whose primary business is lobbying, we display total income and top clients. For organizations that are not primarily lobbying firms, we display total amount spent on lobbying and top lobbying firms hired.
For more information, please see our lobbying methodology page.
Lobbying on Behalf of National Cattlemen's Beef Assn
Names of Lobbyists
Firm Hired Amount National Cattlemen's Beef Assn $336,187 Texas Cattle Feeders Assn $320,000
Most Frequently Disclosed Lobbying Issues
- Agriculture,
- Clean Air & Water,
- Fed Budget & Appropriations,
- Taxes,
- Trade,
- Animals,
- Environment & Superfund,
- Energy & Nuclear Power,
- Food Industry,
- Natural Resources
Most Frequently Disclosed Bills
Bill No. Title H.R.1259 Death Tax Repeal Permanency Act of 2011 H.R.1996 Government Litigation Savings Act S.1061 Government Litigation Savings Act S.1129 Grazing Improvement Act of 2011 H.R.763 Safe and Efficient Transportation Act of 2011 H.R.1633 Farm Dust Regulation Prevention Act of 2011 S.747 Safe and Efficient Transportation Act of 2011 H.R.4234 Grazing Improvement Act of 2012 H.R.910 Energy Tax Prevention Act of 2011 H.R.1287 3-D, Domestic Jobs, Domestic Energy, and Deficit Reduction Act of 2011
RegulationsMentioned in 39 dockets; Submitted to 27 dockets
All data is based on documents downloaded from Regulations.gov. The first table shows mentions: all documents that include the name of the company anywhere in the document or document metadata. The second table shows submissions: all documents where the submitter metadata included the company name. Each table shows the top 10 dockets, ranked by number of occurrences.
Matches are based on a search for the company name. Variations in the company name, such as acronyms, nicknames or alternate names may cause documents to be missed. The mention of a company name in a document may be incidental and does not necessarily indicate that the company has any relevance to the document. Company names that are common English words may erroneously match with text that is not referring to the company.
Not all agencies submit public comments to Regulations.gov. For a list of participating and non-participating agencies see here. Agencies that do submit to Regulations.gov have varying levels of accuracy and completeness.
Regulations and public comments can be downloaded in bulk here.
The tables show occurrences of "National Cattlemen's Beef Assn" in public comments on proposed federal regulations.
Documents Submitted by the Organization
- Submissions
- Agency
- Docket
- Date
View all submissions data for National Cattlemen's Beef Assn
- Toggle 2 FWS Regulations Governing Designation of Critical Habitat Under Section 4 of the ESA, 50 CFR 424 2012
- Toggle 2 FWS 50 CFR 402: Definition for "destruction or adverse modification"; Proposed Endangered Species Act Implementing Regulations 2011
- Toggle 2 FWS Policy regarding implementation of section 4(b)(2) of the Endangered Species Act 2011
- Toggle 1 USTR Request for Comments on Mexico's Expression of Interest in the Proposed Trans-Pacific Partnership Trade Agreement 2011
- Toggle 1 NOAA Endangered and Threatened Wildlife and Plants: Impact Analyses of Critical Habitat 2012
- Toggle 1 USTR Request for comments on SPS Measures 2012
- Toggle 1 APHIS Cattle Fever Tick; Importation Requirements for Ruminants From Mexico 2012
- Toggle 1 FWS Lesser Prairie-Chicken, Proposed Threatened Status 2012
- Toggle 1 USTR US-EU Regulatory Compatability 2012
- Toggle 1 USTR Request for Comments and Notice of Public Hearing Concerning China's Compliance with WTO Commitments 2012
Mentions in Document Text
- Mentions
- Agency
- Docket
- Date
View all mentions data for National Cattlemen's Beef Assn
- Toggle 9 EPA Definition of "Waters of the United States" Under the Clean Water Act 2011
- Toggle 5 WHD Child Labor Regulations, Orders and Statements of Interpretation; Child Labor Violations—Civil Money Penalties 2011
- Toggle 5 EPA National Pollutant Discharge Elimination System Concentrated Animal Feeding Operation (CAFO) Reporting Rule 2011
- Toggle 2 EPA Regulatory Flexibility Act (RFA) Section 610 Review of NPDES Permit Regulation and Effluent Limitations Guidelines Standards for CAFOs 2012
- Toggle 2 FDA Current Good Manufacturing Practice and Hazard Analysis and Risk-Based Preventive Controls for Food For Animals 2011
- Toggle 2 FWS Regulations Governing Designation of Critical Habitat Under Section 4 of the ESA, 50 CFR 424 2012
- Toggle 2 FDA Antimicrobial Animal Drug Sales and Distribution Reporting 2012
- Toggle 2 FMCSA Pilot Program on NAFTA Long-Haul Trucking Provisions 2011
- Toggle 2 FSIS New Analytic Methods and Sampling Procedures for the United States National Residue Program for Meat, Poultry, and Egg Products 2012
- Toggle 2 FWS 50 CFR 402: Definition for "destruction or adverse modification"; Proposed Endangered Species Act Implementing Regulations 2011


text:
St Mary the Virgin, Easton Neston
- About Us
- Find Us
- Contact Us
- Services and Events
- Features and Facilities
- More Info
The parish church of St Mary the Virgin served the old village of Easton Neston which was removed in the 18th century.
The parish today includes the stately home called Easton Netson, its farms, the hamlet of Hulcote, and the new Shires estate on the northern edge of the town of Towcester.
The church building is a beautiful mediaeval church with a tower and inside are some superb monuments of the Fermor-Hesketh noble family which owned the estate from the sixteenth until the early twenty-first centuries, including the life-size alabaster tomb of Sir George Fermor and his wife Elizabeth.
Regular Services are once a month at 6pm on the first Saturday, Easter Eve, Advent Sunday, and Christmas Eve.
Occasional services include weddings, baptisms, funerals, and musical services.
The parish is part of the Tove Benefice. www.tovebenefice.org.uk
Parish boundary view Bird's eye view
You are seeing this message instead because your browser has not loaded the program code to produce the map.
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text:
Picked up this frame from ebay. It is the 1st bike for the year N=N+1 :-)
It has been repainted or powder coated and modernized so the component vintage is not that helpful in determining the frame. There is a serial no under the BB and it fits the Centurion format. Starts with M followed by 5 but it it is very faint it could also be a 3.
Some distinguishing features:
1- The rear dropouts are Suntour GS.
2- Wrap around seat stays
3- Long top tubes lugs
4- Seat post is SR Royal 26.8mm
It looks to be chrome plated underneath the paint but not 100% sure of that either.
I suspect its a Semi Pro but the 5 doesn't match.
Here are the pictures. I reach out to the collective wisdom of this forum for the final determination.
If indeed there is chrome under the lugs what is a good way to expose the chrome again.
Thank you for your help.


text:
Bioinformatics Solution Provider to Support High Throughput Molecular Data Analysis and Result Sharing across Kluyver's European Academic and Industrial Research Sites
Basel, Switzerland (PRWEB) March 23, 2010 -- Genedata, a leading provider of advanced software solutions for drug discovery and life science research, today announced a five-year-extension of an ongoing collaboration with the Netherland's Kluyver Centre, an industry and government consortium using microbial genomics to improve industrial fermentation processes. The Genedata Phylosopher biological data management platform and the Genedata Expressionist biomarker discovery platform will support central data storage and provide automated workflows to integrate transcriptomic, proteomic and metabolomic data for the centre's academic and industrial research partners.
"We are able to pool genomics expertise across the entire Kluyver Centre network using Genedata's standardized and integrated informatics platform," explained Prof. Han De Winde, business director of the Kluyver Centre. "Our research focuses on the development of a complete systems biology workflow, which is aimed at enhancing fermentation in yeast, filamentous fungi or lactic acid bacteria.
|Source: PRWeb
Copyright©2010 Vocus, Inc.
All rights reserved


text: On 04 Jun 2004 21:47:46 -0400, David Steuber <david@...> wrote: > I have a bit of code that parses a Lisp list of forms and does this if > it encounters an appropriate form (in a COND) > > ((fboundp (first form)) (apply (first form) (rest form))) > > The compiler makes a note about not being able to optimize away > possible call to FDEFINITION in the APPLY. That note goes away when > I change the form to look like this: > > ((fboundp (first form)) (apply (the function (first form)) (rest form))) > > However, I get kicked into the debugger when I parse a form that > looks like this: > > (print 1) > > The compiler complains that PRINT is not a function. > > If I leave out THE and ignore the note, the code will recognize PRINT > as a function and APPLY it properly. > > Is this a bug? Xor am I doing something wrong? In the general case of (FUNCALL X ...) the compiler doesn't know if X is a function object or a symbol. That's where the optimization note comes from. If it can be sure it's not a symbol (which it has to look up to get the function it refers to) it can optimize the FUNCALL. That's why the note goes away if you promise to the compiler that (FIRST FORM) will be a function object. However, you are lying - in the case of (PRINT 1) (FIRST FORM) is /not/ a function object but the symbol PRINT. Bummer! Maybe SYMBOL-FUNCTION is what you want here. I don't know the rest of your code. HTH, Edi.


text:
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(Program 10696)Guest: Dr. Edward SriWhat must it have been like on that singular day when Heaven came to speak to the youn...-1075
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(Program 10846)Guests: Anthony EsolenPope Leo the Thirteenth explained that Catholic social teaching offers a unified worl...-1198
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(Program 10847)Guests: Anthony EsolenOne of the most contentious issues facing our culture today is that of marriage - wha...-1199
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(Program 10861)Guests: Father Shenan BoquetThe contemporary culture has not been kind to recent pontiffs and many of the s...-1193
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(Program 10863)Guests: Father Shenan BoquetI am fairly confident that our programs this week have offended the sensibiliti...-1195
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(Program 10787)Guests:Susan Tassone, Father John Grigus, Father Daniel CambraHoly Mother Church tells us that only perfect...-1098
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(Program 10788)Guests:Susan Tassone, Father John Grigus, Father Daniel CambraThe Church teaches us that there are three ca...-1099
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(Program 10789)Guests:Susan Tassone, Father John Grigus, Father Daniel CambraIn our last episode we discovered that the Ch...-1100
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By David Thomas on August 22, 2010
Speaking of 2010 models, this week's podcast features three 2010s, and perhaps after you take a listen you'll want to see if there are any Labor Day deals on them. Or maybe you won't. Listen to editors Mike Hanley, Kelsey Mays and David Thomas offer their unvarnished impressions.
You can download the podcast now via iTunes here or get an MP3 version here.
We've also fixed a glitch that prevented downloading via iTunes on your iPhone.
2010|Lexus|GX 460
2010|Dodge|Grand Caravan
Managing Editor David Thomas has a thing for wagons and owns a 2010 Subaru Outback and a 2005 Volkswagen Passat wagon. Email David