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Clomiphene Citrate – Impending Shortage
The Therapeutic Goods Administration (TGA) has provided medicine shortage information on clomiphene citrate in the Australian market. This information is provided to assist health professionals and consumers when there is a temporary or permanent disruption (discontinuation) to the supply of a medicine in Australia. Read more.
17 July 2010
For Immediate Release
The Royal Australian and New Zealand College of Obstetricians and Gynaecologists (RANZCOG) has today officially released a statement on swine flu for all pregnant women and has advised them to be alert to the possibility of infection and seek appropriate medical help if they have concerns about themselves or the health of their pregnancy.
The College statement, available on the RANZCOG website: 17 July 2009 Swine Flu and Pregnancy Statement (163.41 kB), emphasises that basic personal hygiene measures are the most important strategies in the prevention of H1N1 influenza in pregnancy, suggesting that simple hand washing and covering the mouth and nose when sneezing or coughing and hygienic disposal of tissues are all simple but effective measures.
'Pregnant women are an at-risk group and efforts should be made to avoid those situations where transmission might occur such as in crowded areas or in the workplace', said RANZCOG President Dr Ted Weaver.
'If women are very concerned that they may contract the infection or have other risk factors such as, cigarette smoking, asthma or other chronic diseases, they could take further precautions such as wearing a face mask. This is not essential if the exposure risk is low.
'In an event where a woman does contract swine flu, it is likely that the benefits of anti-viral treatment (e.g. tamiflu) will outweigh any theoretical risks', said Dr Weaver.
The Royal Australian and New Zealand College of Obstetricians and Gynaecologists (RANZCOG) is dedicated to maintaining the highest possible standards in obstetrics and gynaecology in Australia and New Zealand.
For further information or to arrange an interview with Dr Weaver, please contact:
Julia Serafin
Media & Communications Senior Coordinator
(w) 03 9412 2961
(m) 0488 367 476
Copyright © 2011 Royal Australian and New Zealand College of Obstetricians and Gynaecologists. ABN: 34 100 268 969


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James Darren
James William Ercolani, 8 June 1936, Philadelphia, Pennsylvania, USA. This photogenic actor/singer was signed to Columbia Pictures in the mid-50s, his first movie being Rumble On The Docks in 1956. He first sang in Gidget in 1959, from which the title song made the US chart. Other notable starring roles include Because Theyre Young in 1960 and the epic The Guns Of Navarone in 1961. In 1962 he reached the US Top 5 with his lucky 13th single Goodbye Cruel World and followed it with two more transatlantic hits Her Royal Majesty, a Carole King song, and Conscience, written by Barry Mann. Darren later recorded with little success on Warner Brothers Records in 1965, Kirshner in 1969, Buddah Records in 1970, MGM Records in 1973, Private Stock in 1975 (where he returned briefly to the US Top 100 in 1977 with You Take My Heart Away, from the hit movie Rocky) and RCA Records in 1978. Darren is now best known for his...

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great place for family with kids. Second weekend Of every March ostrich festival, great food, rides and much more
Your coupon (that we'll email you) gets you 20% off when you spend at least $250 on tours, activities and more that you can book on TripAdvisor or Viator. The more you spend, the more you save!
To learn more about this Book on TripAdvisor Bonus, Viator, and the full list of official rules, click here.


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There are market makers as well that will buy ones stock back cheap if desperate to sell.But if this were to goto 16 soon which it probably will nobody will want stock from 17.Lots of people wanted Lehman Brother and Enron stock the week and month before it was a formal bankruptcy.That is quite possible here if there is more fraud we do not know about (yet).
You have a wonderful imagination. bankruptcy? Do you think the ToTal deal from Nov. didn't have rpes and warrantees or DD? This Aubry nonsense was designed for max effect on the down. Complete with S&P downgrade. SEC "Informal inquiry" which means nothing criminal happened. The guy is a crook but we know that already and the BoD needs to do something soon.....all spelling relief rally to the 25 range where this belongs.


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The creative and often humorous mid-century modern aesthetic of George Kovacs (1926-2007) made a lasting impression on contemporary lighting. The extraordinary George Kovacs lighting collection, which also includes pieces by Alecia Wesner, Karim Rashid and other mid-century modern lighting designers, is now part of the Minka Group.
The Squared Bath Bar is available with the following:
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A low-profile display of geometric symmetry. Along a narrow steel body, the Mirage Bath Bar by Hinkley Lighting showcases multiple cubed shades made of Etched Opal glass. Small square frames separate each shade at a consistent distance, providing an interesting ...
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A picturesque scene at the church, Lake Tekapo.
via 500px http://ift.tt/1U6gJ1J
A picturesque scene at the church, Lake Tekapo.
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My favorite thing in the world, is the stars. Songs, quotes, movies, anything with stars in it, I will just love it. It is so magical that, something so distant and tiny from our view, can make us realize how small we are in this universe. 264 more words
Time for some sleep :) here's a taste of tonight's Milky Way show down in Tekapo, it didn't disappoint! However I think the tourists that plague this place need a good lesson on Astro Etiquette before they even set foot on the Church grounds. 27 more words
Fifteen Years ago my wife and I where married in this Church. So we are back and it is just as beautiful as we remember, This time i was lucky enough to be able to Photograph it. 140 more words


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- Italian
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Papa Joe's
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May 07 2016
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I came to this place from San Francisco after swimming across the bay with my iguana. I ordered the pepperoni pizza, and when it came I ate it. The cheese...read more
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The best pizza in Napa!! It's crust is delicious and the pizza is always cooked perfectly. When I have a party I always order Papa Joes!! I would highly...read more
Jan 29 2016
Brittany J. via Yelp
This place is awful! It's 4am and I had their pizza for dinner and I'm dying of food poisoning at the moment. Have given this places lots of chances because...read more
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Hematopoietic differentiation critically depends on combinations of transcriptional regulators controlling the development of individual lineages. Here, we report the genome-wide binding sites for the five key hematopoietic transcription factors—GATA1, GATA2, RUNX1, FLI1, and TAL1/SCL—in primary human megakaryocytes. Statistical analysis of the 17,263 regions bound by at least one factor demonstrated that simultaneous binding by all five factors was the most enriched pattern and often occurred near known hematopoietic regulators. Eight genes not previously appreciated to function in hematopoiesis that were bound by all five factors were shown to be essential for thrombocyte and/or erythroid development in zebrafish. Moreover, one of these genes encoding the PDZK1IP1 protein shared transcriptional enhancer elements with the blood stem cell regulator TAL1/SCL. Multifactor ChIP-Seq analysis in primary human cells coupled with a high-throughput in vivo perturbation screen therefore offers a powerful strategy to identify essential regulators of complex mammalian differentiation processes.
► Genome-wide TF binding analysis in primary human megakaryocyte ► Regulatory network reconstruction from TF, epigenetic, and expression data ► Simultaneous binding of five TFs marks hematopoietic regulators ► Five-factor binding reveals eight genes essential for zebrafish blood development
Complex gene-regulatory networks control all metazoan development (Davidson, 2001). Transcription factors (TFs) and the cis-regulatory sequences to which they bind form the building blocks of these gene-regulatory networks. Connectivity in these networks has historically been determined using individual gene-based assays or inferred from expression profiling. However, gene by gene characterization of regulatory elements is slow and inference of regulatory relationships based on variations in gene expression alone cannot readily differentiate between direct and indirect interactions. New genome-wide technologies permit potentially rapid access to all regions bound by a given factor. However, binding of a factor does not necessarily equate with direct regulation of nearby genes. New strategies are therefore required to identify the subsets of binding events that are central to regulatory network function.
Hematopoiesis has long served as a model process for studying stem cells (Orkin and Zon, 2008). One of the earliest branch points during hematopoietic differentiation is the specification of the megakaryocyte (MK)/erythroid progenitor (MEP) (Adolfsson et al., 2005). Subsequent formation of immature MKs leads to the production of large numbers of anucleate platelets. As the second most abundant cell in the blood, their primary role is to maintain hemostasis and instigate wound healing on vascular damage.
Being derived from a common precursor, the MK and erythrocyte lineages share a number of TFs critical for their development, including GATA1, FOG1, SCL, and GFI1b. Other factors, like EKLF (erythroid) and GABPα, FLI1, and RUNX1 (megakaryocytic) have been implicated in the bifurcation of these lineages. Analysis of cell-type specific regulatory elements is consistent with the notion that combinations of these TFs constitute lineage-specific regulatory codes. For example, sites for GATA1 and SCL are found in several erythroid specific enhancers (Anderson et al., 1998; Wadman et al., 1997) and several enhancers active in MKs contain GATA and ETS binding sites (Minami et al., 1998; Yamaguchi et al., 1998).
We have recently reported genome-wide combinatorial interactions for ten key regulators of hematopoietic stem/progenitor cells in the multipotent mouse hematopoietic progenitor cell line 7 (HPC-7) (Wilson et al., 2010a), which revealed combinatorial interactions between a heptad of TFs (Scl, Lyl1, Lmo2, Gata2, Runx1, Erg, Fli1). Subsequent in vivo validation confirmed a previously unrecognized synergism between the three central regulators of blood stem cell development Scl, Gata2, and Runx1. Another elegant study examined the dynamic binding and long-distance regulation by multi-TF complexes during erythroid maturation using a mouse erythroleukemia cell line model (Soler et al., 2010). Furthermore, recent studies have extended genome-wide analysis of key hematopoietic transcription factors to human leukemia cell lines (Fujiwara et al., 2009; Pencovich et al., 2011). However, all the above studies were performed in transformed cell lines. Any findings therefore come with the many caveats associated with the unphysiological nature of immortalized cells. Here we report the genome-wide binding of five key hematopoietic regulators in primary human MKs. Knockdown in zebrafish of eight genes, regulated by all five TFs and with no known function in hematopoiesis, showed all to be essential for thrombocyte and/or erythroid development, suggesting that multifactor chromatin immunoprecipitation sequencing (ChIP-Seq) combined with high-throughput zebrafish screening is a widely applicable strategy to identify regulators of complex mammalian developmental processes.
To determine genome-wide TF binding, primary MKs were cultured from cord blood CD34+ hematopoietic progenitor cells (HPCs; ≥98% pure) for 10 days in the presence of thrombopoietin and interleukin-1β (Figure 1A). 3.3 million CD34+ cells produced a total of 94.7 million primary cells, sufficient for comprehensive genome-wide analysis. After culture, 71%–93% of cells expressed CD41 (Figure 1B) and 20%–56% expressed the more mature marker CD42a (data not shown). Cytospin and modified Wright's stain demonstrated that most cells were at the megakaryoblast stage, however, some pro-MKs with nuclear separation were also seen (Figure 1B). Greater than 90% of cells tested negative for CD34 expression and few cells expressed markers of other lineages (CD11c, 0.4%–4.1%; CD14, 2.4%–2.7%; CD15, 0.7%–2.3%; CD66a, 0.5%–1%; data not shown).
ChIP-Seq technology was then used to generate genome-scale catalogs of sequences bound by GATA1, GATA2, RUNX1, FLI1, and SCL in primary human MKs. Before sequencing, ChIP material was validated by quantitative polymerase chain reaction (PCR). We have previously shown that the RUNX1 +23 enhancer is bound by GATA2, RUNX1, FLI1, and SCL in mouse progenitor cells (Wilson et al., 2010a), and the SCL +40 enhancer by GATA1 in erythroid cells (Ogilvy et al., 2007). As shown in Figure 1C, significant binding of the relevant factors to these two regions was also observed in primary human MKs.
The ChIP material was sequenced yielding between 10.3 million and 14.1 million mappable reads for each factor. Visualization of the raw data across the RUNX1 and TAL1 loci reproduced the PCR results with specific enrichment of the RUNX1 +23 and SCL +40 regions (Figures 2A and 2B, respectively). Inspection of several gene loci for MK-affiliated genes showed that (1) the ITGA2B locus (coding for integrin αIIb, the CD41 marker used to determine the purity of the MK cultures) is bound by all five TFs (Figure 2C); (2) GP9 coding for the MK-specific protein GPIX is bound by all but GATA2 (Figure 2D); and (3) GP1BB is bound by all five TFs just like CD41 (Figure 2E).
To comprehensively identify all binding peaks across the genome, we generated a control IgG ChIP sample to permit the use of software tools specifically designed to eliminate artifactual peaks found in both test and control samples, which allowed us to identify 4722 peaks for GATA1, 2475 peaks for GATA2, 7345 peaks for RUNX1, 8688 peaks for FLI1, and 3085 peaks for SCL (Figure 2F). Over 33% of RUNX1 and FLI1 peaks were situated in promoter regions, whereas for GATA1, GATA2, and SCL this did not exceed 14%.
To obtain new insights into combinatorial TF interactions, we next asked whether the frequency of colocalization of specific combinations of TFs on the same target regions might indicate preferential coregulatory activities. To this end, we determined the number of overlapping peaks for all 26 possible combinations involving binding of two or more factors (Figure 3A, left; see Tables S1 and S2 available online for all peak coordinates and candidate target genes). To address statistical significance, we used a lower end estimate of 80,000 regions potentially available for binding (Boyle et al., 2008; McDaniell et al., 2010), determined the expected frequencies for all 26 binding patterns and calculated significance of deviation between observed and expected values (Figure 3A, right).The most underrepresented pattern was binding by GATA1 and FLI1 without any other factor, suggesting that binding by GATA1 and FLI1 in the absence of larger multi-TF-complexes is not favored. Interestingly, seven of eight combinations involving GATA1 and SCL were overrepresented pointing to the importance of this pairing in megakaryopoiesis (Figure 3A). By far the most significant combinatorial pattern was binding of all five factors together suggesting an important role for this complex in MK transcriptional control. Of note, there was only a minor overlap between the peaks identified here when compared to those from a recently published study where four of the five factors were analyzed in a mouse hematopoietic progenitor cell line (Figure S1), suggesting that cell-type specific binding previously reported for individual TFs (Heinz et al., 2010) extends to multifactor combinatorial binding.
We next used sets of regions with particular TF occupancy patterns for de novo motif discovery, which recovered the expected consensus binding motifs (Figure 3; see Figure S2 for expected consensus motif sequences). For example, analysis of sequences bound only by SCL and GATA1 recovered a typical SCL/GATA1 composite binding motif (Figure 3Bi), also seen in recent ChIP-Seq studies of Scl in erythroid cells (e.g., Kassouf et al., 2010 and Soler et al., 2010) and now recognized as the likely docking site for Scl/Gata complexes. This analysis also recovered the SCL binding E-box motif (Figure 3Bii) as well as a motif resembling an E-box, but not present in current collections of transcription factor consensus binding sites (Figure 3Biii). Analysis of other subsets such as all regions bound by SCL and GATA1 plus one or more of the other three factors recovered additional expected motifs such as GATA, ETS, and RUNX consensus sequences (e.g., Figures 3Biv–3Bvi), thus demonstrating that expected motifs were present in the regions identified by ChIP-Seq analysis.
Having established the potential importance of combinatorial interactions between GATA1, GATA2, RUNX1, FLI1, and SCL, we next investigated the connectivity within the core network formed by these five factors. As shown in Figure 4A, all five TFs are characterized by a positive autoregulatory feedback loop, and in addition contain extensive cross-regulatory links, thus resulting in the formation of a densely connected core circuit. Studies in lower model organisms have suggested that regulatory networks commonly consists of densely connected TF core circuits that control large numbers of tissue-specific effector proteins (enzymes, structural proteins, etc.) (Davidson, 2010; Roy et al., 2010). With occupancy patterns being nonrandomly distributed (Figure 3A), we next investigated the nature of the candidate target genes for all possible 31 occupancy patterns, and therefore mapped TF binding peaks to nearby genes thus generating 31 target gene lists (Table S2). To identify those sets of target genes most likely to be important for the megakaryocyte phenotype, we next performed gene set enrichment analysis (GSEA), which demonstrated that six occupancy patterns were associated with genes highly enriched for MK-specific expression (Figure 4B and Table S3). Of note, four of these six peak occupancy patterns (P1, P2, P3, P5) had also been found to occur much more frequently than expected by chance (see Figure 3A). This convergence between statistical analysis of peak pattern frequency and GSEA analysis of neighboring genes therefore suggests that key aspects of the megakaryocyte transcriptional program can be captured from comprehensive analysis of our data set.
Of note, none of the 31 gene lists showed significant correlation with genes specifically repressed in MKs, thus suggesting that the five-factor core circuit analyzed here largely functions as a positive regulator of MK expression. To further explore this notion, we performed additional ChIP-Seq experiments to generate genome-wide maps indicative of active and repressed chromatin. Trimethylation of histone H3 lysine 4 (H3K4me3) was used to map active promoters, histone H3 acetylation (H3acet) for active promoters as well as more distal elements, and trimethylation of histone H3 lysine 27 (H3K27me3) as a mark for repressed chromatin. All three data sets displayed the expected patterns of enrichment (Figure S3A). We therefore proceeded to categorize all human promoters into three categories (inactive, bivalent and active) based on their pattern of histone modification (Figure S3B). As expected, when gene expression in the three categories was examined, the highest level of expression was found in the category of "active" promoters (Figure S3C). Moreover, the fraction of promoters bound by the five transcription factors was lowest for the inactive promoters, at an intermediate level for bivalent promoters and highest for the active promoters (Figure S3D). Genome-wide analysis of chromatin status was therefore consistent with the notion that the core TF circuit defined here is predominantly involved in positive regulation of gene expression.
With analysis of combinatorial binding patterns, gene set enrichment and chromatin analysis all converging on a consistent role for the GATA1/GATA2/RUNX1/FLI1/SCL core circuit within wider MK transcriptional programs, we next developed a regulatory network model (Lin et al., 2010) illustrating the connectivity of all genes controlled by TF peaks correlating with MK-specific expression (e.g., the gene sets corresponding to patterns P1 to P6 from Figure 4B). As illustrated in Figure 4C, target genes controlled by the six different types of peaks are largely nonoverlapping. This analysis therefore suggests that control of MK effector genes mainly occurs via a single regulatory element whereby several distinct combinations of GATA1/GATA2/RUNX1/FLI1/SCL can be involved in contributing to MK-specific expression. Taken together, comprehensive bioinformatic analysis has allowed us to generate a regulatory network model for megakaryocyte-specific expression with a densely connected core circuit controlling several hundred effector genes through a relatively shallow hierarchy, a network architecture recently predicted to be common for tissue-specific gene expression programs (Davidson, 2010). It is important to note, however, that not unexpectedly, the current five-factor network model does not capture MK-specific expression in its entirety. Of the top 200 megakaryocyte-specific genes, 67 form part of the current network model, thus suggesting that alternative TF combinations mediating MK-specific expression remain to be discovered.
With all five factors binding together being the most significant combinatorial pattern in our data set (Figure 3A) and the most highly enriched for MK-specific genes (Figure 4B and Table S3), we hypothesized that concurrent binding by these five factors represented an important control mechanism for hematopoietic development. To explore this hypothesis further, we used the genomic regions enrichment of annotations tool (GREAT) (McLean et al., 2010), which defines genomic neighborhoods for TF-bound peaks by attaching weights to flanking genes based on their distance to the peak. Weighted gene lists are then used to identify overrepresented biological functions. GREAT analysis for the 144 regions bound by all five TFs showed strong enrichment for a number of phenotypes, all of which were hematopoietic and included decreased platelet count (Table 1). Taken together therefore, concurrent binding of GATA1, GATA2, RUNX1, FLI1, and SCL highlights a set of genes highly enriched for known (and potentially unidentified) regulators of hematopoiesis in general and of megakaryopoiesis more specifically.
To further explore the biological significance of the subset of genes bound by all five TFs, we performed functional studies using zebrafish as a model for high-throughput in vivo validation. Mapping peaks to genes identified 151 candidate target genes for the 144 regions bound by all five TFs of which 24 (16%) have a role in transcriptional control and 28 (19%) are involved in signal transduction (Table S4). Fifty-seven of the 151 genes were (1) expressed in MKs based on a recent compendium of gene expression profiles in primary human hematopoietic cell types (Watkins et al., 2009) and (2) displayed increased expression in a recently published MK differentiation time series data set (Fuhrken et al., 2008). Eighteen of these 57 genes had known hematopoietic functions, and of the remaining 39 genes, zebrafish orthologs could be readily identified for 37 genes. Morpholino antisense oligonucleotides (MOs) were designed against eight of these genes, chosen randomly to provide an unbiased sampling, as well as against the TF nfatc1. Nfact1 was included because even though NFATC1 had been implicated in transcriptional control in lymphocytes (Shaw et al., 1988), there were no prior data suggesting possible functions in megakaryopoiesis (see Table S5 for a summary of selected genes). MOs targeting these nine genes were injected into one-cell stage zebrafish embryos and assayed for their effect on two hematological traits: number of erythrocytes and number of thrombocytes. Remarkably, for all genes except nfatc1, injection of MOs caused a significant reduction in the number of circulating blood cells at 48 hr postfertilization (hpf) (Figure 5A) as confirmed by o-dianisidine staining for hemoglobin production. Whereas emilin1 and sufu MOs had a mild effect on erythropoiesis, ncor2, march2, smox, pttg1Ip, max, and pdzk1ip1l MO injected embryos showed a profound reduction in the number of mature erythrocytes.
To further explore potential hematopoietic functions for the nine candidate genes, we examined cd41 expression at 72 hpf in the caudal hematopoietic tissue (CHT) of MO injected fish using the Tg(cd41:EGFP) transgenic line (Lin et al., 2005) (Figures 5B and 5C; for pictures and cell counts in multiple embryos, see Table S6 and Figure S4). The CHT is widely recognized as the anatomic site of definitive hematopoiesis in zebrafish embryos, and cd41low and cd41high are considered to be the markers of hematopoietic stem cells (HSCs) and thrombocytes, respectively. For six genes (march2, max, smox, pttg1lp, emilin1, and sufu) MO depletion resulted in a severe decrease in the number of thrombocytes and HSCs. Ncor2 depletion resulted in a mild phenotype. Pdzk1ip1l and nfatc1 MO injected embryos showed no change in the number of cd41 positive cells when compared to control embryos. Taken together, in vivo functional validation therefore demonstrated that genes adjacent to DNA sequences concurrently bound by GATA1, GATA2, RUNX1, FLI1, and SCL are highly enriched for previously unknown hematopoietic regulators.
Identification of a hematopoietic function in zebrafish for pdzk1ip1I was of particular interest because (1) its mammalian ortholog PDZK1IP1 (also known as MAP17) encodes a membrane associated protein shown to modulate the levels of reactive oxygen species (Guijarro et al., 2007) that have recently emerged as important determinants of blood stem cell function (Tothova et al., 2007); (2) PDZK1IP1 is situated immediately 3′ of the hematopoietic master regulator SCL (see Figure 6A); (3) this arrangement is conserved between mammals and zebrafish (Gottgens et al., 2002a); and (4) an erythroid enhancer regulating murine Scl expression is located 3′ of PDZK1IP1 (+40 element; Figure 6A) (Delabesse et al., 2005). We previously argued that this Scl 3′ enhancer was the cause for close linkage between Scl and Pdzk1ip1 during evolution because major rearrangements would have interfered with SCL expression (Ogilvy et al., 2007). However, the identification of a hematopoietic function for pdzk1ip1l suggests that the intimate linkage of SCL and PDZK1IP1 might be due to the fact they share regulatory elements to mediate their respective functions in hematopoietic cells. Interestingly, PDZK1IP1 is upregulated during megakaryocyte differentiation from CD34+ HPCs (Fuhrken et al., 2008) and we have previously observed that the Scl +19 enhancer targets expression to megakaryocytes (Silberstein et al., 2005).
To investigate the possibility of coregulation, we first determined expression of Pdzk1ip1 in mouse embryos. As shown in Figure 6B, Pdzk1ip1 was expressed in the midgestation mouse embryo both in the dorsal aorta (AGM region) and fetal liver in the hematopoietic domains that also express Scl. Of note, expression in dorsal aorta endothelium and hematopoietic clusters as well as a subset of fetal liver cells was highly reminiscent of the expression pattern previously seen in transgenic mouse embryos with the +19 enhancer fused to an Scl-promoter-lacZ reporter gene (Sanchez et al., 1999).
To examine hematopoietic expression of Pdzk1ip1 further, we generated embryonic stem cells (ESCs) with a lacZ reporter gene knocked into the ATG start codon of Pdzk1ip1 (Figures 6C, S5A, and S5B) and then used lacZ expression during ESC differentiation as a surrogate for Pdzk1ip1 expression. ESCs were differentiated into embryoid bodies to produce hematopoietic cells that could be analyzed by flow cytometry and colony assays. At day 6 of differentiation, ~25% of embryoid body cells derived from Pdzk1ip1 knockin (KI) ESCs displayed lacZ staining (Figure 6D) with the vast majority of hematopoietic colony forming activity found in the lacZ expressing cells (Figure 6E). Pdzk1ip1 expression therefore marks hematopoietic cells in both mouse embryos and differentiating ESCs within the known expression domain of Scl.
To investigate whether the regulatory elements previously shown to drive Scl expression can also act on the Pdzk1ip1 promoter, we generated transgenic mouse embryos carrying the Pdzk1ip1 promoter with and without the Scl +19 enhancer fused to a lacZ reporter gene. Of 15 transgenic embryos with the Pdzk1ip1 promoter alone, only two showed expression (in the brain and limb buds, respectively). By contrast, 7 of 15 transgenic embryos for the promoter/enhancer construct showed staining, with five of seven embryos displaying strong staining in the fetal liver (Figure 6F) in a pattern highly reminiscent to what we observed previously for the +19 enhancer in conjunction with the Scl promoter (Sanchez et al., 1999). Of note, histological analysis demonstrated that LacZ expressing cells included fetal liver megakaryocytes (Figure S5C). These results demonstrate that a regulatory element previously thought to simply control expression of Scl can act in vivo to mediate expression from the Pdzk1ip1 promoter. However, the Scl +19 enhancer was previously shown to interact with both Scl as well as heterologous promoters (Sanchez et al., 1999). The experiments presented here therefore do not directly prove that the +19 enhancer normally mediates specific Pdzk1ip1 regulation in vivo. Our results are nevertheless suggestive of a model whereby two very different proteins are maintained in close linkage throughout vertebrate evolution because they share key regulatory elements to sustain their expression in hematopoietic cells where they both perform important functions. Similar coregulatory relationships may be common throughout the genome, and thus an important consideration to be taken into account when utilizing ChIP-Seq studies for the reconstruction of regulatory networks.
Detailed molecular studies have shown individual TFs to play critical roles at various stages of MK maturation. For example, a hypomorph mouse model of Gata1 displays reduced platelet numbers associated with deregulated MK proliferation and severely impaired cytoplasmic maturation (Shivdasani et al., 1997). The other TFs investigated in this study, GATA2, RUNX1, FLI1, and SCL, have also been previously shown to play important roles in MK differentiation (Growney et al., 2005; Hart et al., 2000; Huang et al., 2009b; Ichikawa et al., 2004; Mikkola et al., 2003; Song et al., 1999; Spyropoulos et al., 2000). Of note, these four factors also represent key players in regulatory network circuits operating in HSPCs (Gottgens et al., 2002b; Landry et al., 2009; Pimanda et al., 2007) consistent with the previously suggested notion of significant overlap between transcriptional control mechanisms in MKs and HSPCs (Huang and Cantor, 2009).
The five-factor ChIP-Seq data set generated made it feasible for us to perform a global analysis of combinatorial transcriptional control in a human primary myeloid cell type. Previous studies, which only examined individual promoter fragments, had revealed transcriptional cooperativity in MKs between RUNX1 and GATA1 (Elagib et al., 2003; Xu et al., 2006) as well as between FLI1 and GATA1 (Huang et al., 2009a). The latter was somewhat surprising given the well characterized antagonism between GATA1 and ETS family TFs during erythroid differentiation. Our genome-wide data sets support frequent co-occupancy of GATA1 and FLI1 in MK cells with a total of 1335 regions bound simultaneously by both factors. However, the vast majority (1094) of these are bound by additional factors and binding of GATA1 and FLI1 without any of the other factors was in fact the most underrepresented occupancy pattern. These observations suggest that activatory interactions between GATA1 and FLI1 may at least in part be mediated through the assembly of larger multiprotein complexes. Of particular relevance may be the observation that >700 regions are bound by GATA1 and FLI1 together with RUNX1. RUNX1 has been shown previously to interact at the protein level with both GATA1 and FLI1 (Huang et al., 2009a; Elagib et al., 2003). Moreover, RUNX1 expression is maintained in MKs whereas it is downregulated during erythroid differentiation (Elagib et al., 2003). Our combinatorial binding data are therefore consistent with a model where additional factors such as RUNX1 mediate transcriptional cooperation between GATA1 and FLI1 in MKs whereas antagonistic interactions may prevail in their absence as previously reported for the erythroid lineage.
Genes next to regions bound by all five factors were highly enriched for known regulators of MK differentiation and/or function. This observation prompted us to investigate the possibility that genes of unknown hematopoietic function with binding peaks for all five factors may also be enriched for the same functional categories. To provide biological insight, genome-scale hypothesis-generating screens such as ChIP-Seq experiments need to be coupled with meaningful assays for downstream functional validation. Here we took advantage of the zebrafish model because hematopoietic control mechanisms are highly conserved between zebrafish and human/mouse, yet only the zebrafish allows relatively high-throughput knockdown analysis in vivo in the context of a whole animal. Coupling the multifactor ChIP-Seq screen with zebrafish in vivo validation allowed us to identify eight regulators of thrombopoiesis and/or erythropoiesis. Of note, none of the MOs caused a lack of blood circulation and at the concentration used, with the exception of two (smox and max), MO knockdown did not affect wider aspects of morphology, underscoring the hematopoietic specificity of the uncovered phenotypes. Given that seven MOs affected both erythrocytes and thrombocytes, it is possible that at least some of those genes may operate at the level of immature progenitors or blood stem cells. Given the overlap of key transcriptional regulators in MKs and HSCs, potential functions in blood stem cells might perhaps not be too surprising but will require further fine dissection of knockdown phenotypes.
Recent ChIP-Seq studies of individual transcription factors in two different hematopoietic lineages suggested that binding patterns are largely lineage-specific (Heinz et al., 2010) with shared peaks in one study of Scl being as low as 6% (Palii et al., 2011). Comparison of the megakaryocyte data generated here with a recently published hematopoietic progenitor data set (Wilson et al., 2010a) confirmed the predominance of lineage-specific binding patterns, and in addition demonstrated that regions bound by multiple factors are also largely lineage-specific. This suggests that additional lineage-specific transcription factors may play a part in controlling cell-type specific accessibility of regulatory regions through interactions with chromatin modifying enzymes. The likely relevance of additional MK-specific transcription factors is further emphasized by our observation that the five factors studied here may only account for 30% of MK-specific expression. Additional important players within MK transcriptional programs are likely to include NF-E2, MEIS1, and E2A (Shivdasani, 1996; Hisa et al., 2004; Semerad et al., 2009). Our demonstration that PDZK1IP1 shares transcriptional regulatory elements with the blood stem cell regulator SCL has implications reaching beyond a better understanding of this particular gene locus. The notion that a given regulatory region can control multiple genes is well established, but past experimental analysis has largely focused on clusters of related genes such as the β-globin locus (Tolhuis et al., 2002) or tandem arrays of interleukin genes (Loots et al., 2000). Coregulation within these gene loci is likely to have arisen in parallel with local gene duplication events resulting in coordinated control of evolutionary and functionally related genes. By contrast, SCL and PDZK1IP1 encode unrelated proteins and it is likely that their original juxtaposition within vertebrate genomes was accidental. Our identification of PDZK1IP1 as a hematopoietic regulator together with the demonstration that SCL and PDZK1IP1 share transcriptional enhancer elements provides a rationale for the tight linkage between these two genes throughout vertebrate evolution. Moreover, our data illustrate the potential pitfalls of mapping transcription factor binding events to a single target gene when reconstructing regulatory networks. The +19 enhancer is approximately equidistant to the SCL and PDZK1IP1 promoters and evidently can control both. Transgenic in vivo analysis as performed here will be too time consuming and costly for global mapping of regulatory interactions between distal enhancers and the promoters of neighboring genes. However, further reductions in DNA sequencing costs may mean that chromatin-capture based methods providing a genome-wide view of promoter/enhancer interactions (Fullwood et al., 2009) will eventually perform this integral part of regulatory network reconstruction.
Previous multi-TF ChIP-Seq studies have revealed layers of information that can only be obtained from integrated analysis of multiple factors. For example, computational analysis of a 12-factor ChIP-Seq data set from mouse ESCs suggested that genome-scale analysis of combinatorial TF occupancy has the potential to predict absolute and differential gene expression (Ouyang et al., 2009). A five-factor ChIP-Seq study in an erythroid model cell line revealed that multi-TF complex binding often marks sites of long-range genomic interactions (Soler et al., 2010). Here we have shown that multifactor ChIP-Seq surveys coupled to high-throughput in vivo functional screening provide a powerful strategy toward isolating key regulators of cellular phenotypes. Future detailed functional analyses of the eight hematopoietic regulators identified here, has the potential to integrate new regulatory pathways into an emerging framework of blood development.
Cord blood was obtained after informed consent under a protocol approved by the National Research Ethics Service. CD34-positive cells (≥98%) isolated by magnetic cell sorting (Myltenyi Biotec), were seeded at 1 × 105 cells/ml in CellGro SCGM medium (CellGenix) with 100 ng/ml human TPO (CellGenix) and 10 ng/ml IL-1β (Miltenyi Biotec) and incubated for 10 days. Flow cytometry was performed on a CyAn ADP 9 color (Beckman Coulter) as described (Macaulay et al., 2007) using the following antibodies: CD11cPE-Cy5 or V450 (clone B-ly6; Becton Dickinson [BD]), CD14PB (clone M5E2; BD), CD15APC (clone HI98; BD) or V450 (clone MMA; BD), CD34PE (clone 581; Beckman Coulter), CD41APC (clone HIP8; BD), CD42aF (clone ALMA.16; BD), CD66PE (clone B6.2; BD), and IgGAPC (clone MOPC-21; BD).
ChIP assays were performed as previously described (Forsberg et al., 2000) with 9.4–12.6 × 106 cells and anti-GATA1 (ab11963, Abcam), anti-GATA2 (clone H-116, Santa Cruz), anti-RUNX1 (ab23980, Abcam), anti-FLI1 (ab15289, Abcam), anti-TAL1 antibody (clone C-21, Santa Cruz) and nonspecific Rabbit IgG (I5006; Sigma Aldrich). Samples were sequenced using the Illumina GII Genome Analyzer. Sequence data have been submitted to http://www.ncbi.nlm.nih.gov/geo (GEO record GSE24674).
Three peak finding programs (Peakseq [Rozowsky et al., 2009], Findpeaks [Fejes et al., 2008] and model-based analysis of ChIP-Seq [MACS] [Zhang et al., 2008]) were used, based on previous observations that there is no single algorithm of choice when calling peak regions for different TFs (Laajala et al., 2009). De novo motif discovery was performed using MEME (Bailey and Elkan, 1994) or bioprospector (Liu et al., 2001) and motifs were compared with the JASPAR_CORE database (Bryne et al., 2008). Matches to consensus sequences were determined as described using TFBSSearch (Chapman et al., 2004). Peaks in promoters and introns were allocated to that gene and the remainder of peaks to the nearest 3′ and 5′ genes in a 100 kb region. Intersects of gene lists were generated using Galaxy (Goecks et al., 2010), gene ontology terms were analyzed using GO Term Mapper (Lewis-Sigler Institute for Integrative Genomics at Princeton University), and gene set enrichment analysis was performed using the GSEA software (Subramanian et al., 2005).
General maintenance, collection, and staging were carried out according to the Zebrafish Book (Westerfield, 1994). Antisense MOs (Gene Tools) were designed complementary to the 5′ sequence near the start of translation (max and sufu), or at splice junctions (ncor2, march2, smox, nfatc1, pttg1lp, emilin1, and pdzk1ip1l) (Table S7). An aliquot of 0.8 nl morpholino-containing solution was injected in zebrafish embryos at the one- to two-cell stage. For march2 and pdzk1ip1l a MO concentration of 3 μg/μl and for ncor2, smox, nfatc1, pttg1lp, max, emilin1, and sufu a MO concentration of 6 μg/μl was used. Efficiency of splice-site MOs was determined by RT-PCR (Table S7). Total RNA was isolated from control and splice MO injected embryos using the RNeasy Mini Kit (QIAGEN) and cDNA synthesized using SuperScript III (Invitrogen). Staining of hemoglobin by o-dianisidine was performed as described (Detrich et al., 1995). Photomicrographs were taken with a Zeiss camera AxioCam HRC attached to a LeicaMZ16 FA dissecting microscope (Leica Microsystems).
To generate Pdzk1ip1 KI ESCs a targeting vector was generated by bacterial recombineering (Liu et al., 2003) where 131bp of exon 1 of Pdzk1ip1 (containing the ATG site) were substituted by a LacZ reporter gene and a LoxP-PGK-Neo-LoxP cassette. Targeting was determined by Southern blot using 5′ and 3′ probes outside the targeting vector. Targeted cells were transiently transfected with PGK-Cre for deletion of the Neo cassette (Figure S5). Pdzk1ip1 KI ES were differentiated into embryoid bodies as described (Keller et al., 1993) and stained with X-gal as described (Smith et al., 2008). For sorting, embryoid bodies were disrupted at day 6 of differentiation and stained with Fluorescein di(β-D-galactopyranoside) (FDG) (Sanchez et al., 1999). Cells were plated in methocult (Stem Cell Technologies) with appropriate cytokines (Peprotech, R&D) and scored after 7 days.
Pdzk1ip1-Pr/Lac and +19/Pdzk1ip1-Pr/Lac transgenic constructs were generated by inserting a 4.5 kb fragment containing the Pdzk1ip1 promoter and a 640 bp fragment containing the +19 enhancer into the pGLac vector. Methods for transgenic mice generation and in situ hybridization are described elsewhere (Wilson et al., 2010b).
We thank S. Charnock-Jones and staff at the Rosie Hospital for their help obtaining cord blood samples, G. Kiddle for help with sequencing, and A. Rendon for help with mapping ChIP-Seq reads. M.R.T. was supported by the Netherlands Organisation for Scientific Research (NWO; 2008/05927/ALW) and is currently a Marie Curie Intra-European Fellow (237296). A.C. and D.L.S. are supported by the Wellcome Trust (grants 082597/Z/07/Z, 077037/Z/05/Z, 077047/Z/05/Z). W.H.O. and P.A.S. are funded by the National Institute for Health Research/NHS Blood and Transplant (RP-PG-0310-1002). R.F., A.F., and A.R.G. are supported by the Wellcome Trust and K.O. and S.H.O. by the Kay Kendall Leukaemia Fund. Work in the Göttgens lab is supported by Leukaemia and Lymphoma Research, the Leukaemia and Lymphoma Society, the Medical Research Council, and the National Institute for Health Research Cambridge Biomedical Research Centre.
Sequence data have been submitted to the NCBI Gene Expression Omnibus database (accession number GSE24674).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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I was thrilled when AmoroK played all my iTunes files from my Windows partition, however if I try to import aac's it comes back with nothing, except a few mp3's i have from my days with Windows Media Center... It act's like it doesn't know the format even though it plays. I really want to use linux for everything i do on my computer, and i do, except for putting music on my ipod. If there is no way to get my aac's to work properly in AmoroK then i would appreciate it if someone told me how to convert them to them to oggs,
because i could then put linux on my ipod and be happy... (http://ipodlinux.org/Main_Page).


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Have you ever burnt the side of your arm on an oven when turning a dish so it cooks evenly? Not only is this a safety hazard, but it slows service down while you are taking care of your burn. Tongs have many benefits, one of which is giving a person the extra reach you need when reaching into the oven.
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MTX ThunderForm Unloaded Enclosure
Holds two 10" subs fits select 2000-up Ford SuperCab pickups (Tan)
Item #: 236250T20U
In Stock
$349.99 Earn up to 350 Rewards points
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Our take on the MTX ThunderForm Unloaded Enclosure by Crutchfield's Jo Greene
Crinkled french fries, discarded receipts, old gum wrappers you probably have everything in the hidden corners of your vehicle except great bass. ThunderForm enclosures fill the nooks and crannies of your vehicle with low tones that stand out, but their factory look blends right in.
MTX engineers evaluate the "sonic signatures" of top-selling vehicles so they can design ThunderForms as custom acoustic matches. Each ThunderForm fits and blends inconspicuously into your vehicle, so you get great bass without losing any space. They're made of high-density polyethylene resin to curb resonances they deliver bass that's tight, hard-hitting, and way down low. The airtight enclosure provides the correct internal volume to yield optimum bass response.
This enclosure comes without speakers you install two of your favorite 10" subwoofers. For best results, MTX strongly recommends using a speaker designed for an enclosure with a volume of 0.58 cubic feet. The enclosure mounts under the rear bench seat of select 2000-up Ford SuperCab pickups. The jack stays in the factory location, and tools are stored under the enclosure.
Please check with us before purchasing by using the vehicle selector on our site, or calling an Advisor at 1-888-955-6000 to verify fit information.
What's in the Box:
- Custom-fit unloaded dual 10" subwoofer enclosure
- Hardware
- Installation instructions
Product Highlights:
- custom-fit tan subwoofer enclosure for two 10" subs
- mounts under rear bench seat
- fiberglass construction
- volume: 1.17 cubic feet (0.58 per sub)
- dimensions: 6-9/10"H x 55-1/8"W x 10-3/4"D
- mounting depth: 5"
- warranty: 2 years
- Our 60-day money-back guarantee
MTX ThunderForm Unloaded Enclosure Reviews
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What's in the box?
Download the MTX ThunderForm Unloaded Enclosure manual
- Custom-fit unloaded dual 10" subwoofer enclosure
- Hardware
- Installation instructions
Features and Specs
|Box Type
|Number of Boxes
|Number of Speakers Per Box
|Speaker Size
|Cutout Diameter or Length (inches)
|9 3/16
|Maximum Speaker Depth (inches)
|Box Volume (cubic feet)
|Tuning Frequency
|Height (inches)
|Width (inches)
|Depth (inches)
|Second Depth (inches)
|Wood Thickness (inches)
|Speaker Wire Connectors
|Spring posts
|Box Color
|Box Finish
|Parts Warranty
|2 Years
|Labor Warranty
|2 Years
Product Research
Vehicle Compatibility: This Bass Slammer custom-fit enclosure is designed specifically to fit the 2000-2010 Ford F-250/350 Super Cab pickup truck.
Note: Please refer to the Crutchfield Vehicle Selector to confirm fit for your vehicle.
Mounting Location: Then enclosure mounts behind the rear seats, firing down, in Ford F-250/350 Super Cab trucks. The vehicle's jack stays in its factory location and tools relocate to under center of enclosure.
Enclosure Type: The sealed enclosure features fiberglass construction and fits two 10" woofers.
Tools For Installation:
- Powered screwdriver
- 6" Bit holder extension
- #2 Phillips head bit
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Customer Q&A
1 question already asked
1 answer What sub and amplifier recommended for this Enclosure? And is there room behind order with this for an amplifier [ Michael Aug 20, 2015 ]
I forwarded your question to an Advisor and they responded with the following: You can use almost any 10" sub and amp combo that you want, just make sure the sub works with .58 cubic feet of air space and is no more than 5" deep. The box wasn't made with anything specific in mind so it just comes down to your budget and the level of performance you want. Due to lack of airflow I would not put an amp behind it as this could cause it to overheat. If you would like to discuss further you can contact an Advisor directly by phone (1-800-324-9695) or online chat. [ Krissy Aug 24, 2015 ] Staff


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Ramada Inn Resort Eastgate
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
- Room service
- Swimming pool
- Wheelchair accessibility
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Historical Traveler Reviews Ramada Inn Resort Eastgate Kissimmee
Old hotel
My Daughter and i stayed in the ramada christmas eve 2003 for a 3 night stay. The hotel is old and the rooms are in need for a remodel. We had no prolbems with the elevators has great acess to old town and great serivce for shopping and to the major attractions. The on site restraunt BEWARE!!!!!!!!!! verry discusting you will be better eating of site at the Las Vegas Buffet. Also at the poolside bar isn't really poolside it is more like in the hotel with a view of the pool. if you order a hot dog for your kids don't do it the price is $4.00 for one.
The lakeside is more like a swamp. They do have a pavillion and swings that rock like the ones on a front porch they are verry old and look like they are about to fall apart. This hotel is within greatr access to everything and easy walking distance to old town and not that far from disney's town of celebration. the pool is great and so is the hot tub other than older rooms your family should stay here .
What a dump!!!!
I booked this hotel for a two night stay after looking at the virtual tour offered. What a joke that was. The room was a dump, stains on the walls, mattresses, curtains, peeling wallpaper in the bathroom. The water barely came out in a trickle in the bathroom faucet. The top security lock was broken, the hairdryer was broken, worn and dirty comforter on the beds. Disgusting!!!!! The fire alarms on the second floor went off at 10:30 at night and when I called down to the front desk, I was told that there was probably not a problem, but it was our perogitive if we wanted to leave the building. I had prepaid for two nights, but I left the following morning, and it was worth losing my money to find a clean hotel. I would never go to any Ramada Inn anywhere because of this experience.
We wished we were somplace else!
we booked the hotel based upon the description and the picture on Hotels website. What a disappointment. You are advised to step to the next window after you're checked in to get your 'welcome kit'. The welcome kit includes discount tickets to Disneyworld, with a catch - you get a free breakfast and then have to sit through an hour and half presentation about time shares. The lady at the welcome booth ought to be a used car salesman - she talked so fast that you weren't exactly certain about the time share deal unless you were paying close attention! Just what I want to do on my vacation! (not!) The room was a joke, flaking paint in the bathtub, old dirty box spring mattresses, mold on the celing, etc. It looked like a $20 a night room. They're 'all you can eat seafood buffet' was crap. We won't be going back.
Great for the price
We just returned from our 8 day stay at the Ramada Eastgate. We
traveled in end of Nov/ begining of Dec and the prices were good 49.50
(expedia) for a pool view kidsuite. We had a microwave and fridge which
helped us with breakfast and sandwiches and snacks. Room was smaller
than expected with the name 'suite' in it. Nice balcony- could have
used furniture on them. Large clean pool that the kids loved, nice hot
tub for adults. The room was a little old but was good for the price.
Sheets were old and scratchy but service was excellent. Nice to have a
paper in the morning and a safe in your room.
We would stay again at our bargain/off season rate but not at higher/in season rates.
Decent price, decent hotel
Just returned from our six day stay at the Ramada Inn Resort Eastgate. Our room showed signs of age (flaking paint in bathroom, worn carpet and a light that only worked part time). But, all in all, we were happy with the hotel. The room was clean, the staff was very friendly and helpful and the swimming pool area was terrific. We booked a "package deal" and felt it was good value for what we paid. One note, however, if you book via the Internet, be sure to take copies of all your emails with the hotel. On checkout, they were going to charge us the regular rate but I had the reservation confirmation with me and it reduced the bill by about $500.
Good Experience at Ramada Eastgate
My husband and I stayed here in May 2003 for six days. Overall, the hotel suited our basic need of having a place to sleep after visiting the parks all day. The air-conditioner unit did not work in our original room, and it was really noisy having a room by the pool, so we asked to be moved. They moved us without any problem, and our next room was fine and very quiet at the end of the hall. It had a king size bed (not a very comfortable one though), a couch, coffee table, desk, two sinks and a balcony. The maids did a good job cleaning each day. We did not eat at the restaurant because there were numerous chain restaurants along highway 192. We didn't use the pool or any of the activities such as the tennis court. They provided a newspaper each day (you pay for this in the resort fee), and it had an on-site laundry room. The elevator wasn't air-conditioned, smelled bad and sounded like it would break at any moment. The people at the front desk were courteous and set a wake-up call for us each morning. This hotel is in a good location to the parks and the area seemed very safe. It is not fancy, but if you get a good rate for a room (we paid $35/day), I think it is worth that.
OK but just
The location is excellent. Easy enough to get to and close to
everywhere we wanted to go. The room was large enough for 4, the maids
worked hard and did their best to make a fusty smelling room with damp
on the ceiling look good but it was abit off putting. The buffet
breakfasts were good value but never wnet near the sea food dinners as
they seemed expensive unless you're into crab etc. Overall I probably
was paying ecomony and it felt that way. They have a push on some sort
of timeshare and you get cheap tickets but I didn't check it out.
Toilet blocked a couple of times but they sent someone around
immediately to sort it out. Hate that happening in the first place
Couple of neighbours thought they would practice their basketball on the floors and walls during the night but security sorted that out very quickly once someone complained. Perhaps its hit and miss with the rooms but I feel it should have been better.
A Great Price Value
We had come down on our annual family vacation to find a great price value close to Disney. We found the Ramada to be convenient to Disney by car (just up 192) and nearby to the other parks and attractions as well. The staff at the hotel were friendly and helped us with getting around the area quickly. Our room was only $49.99 with our own private balcony overlooking the pool. The room was clean and had two full beds.
The buffet breakfast was great and much cheaper than would you pay at Disney for food.
Beware of the Ramada eastgate
I stayed at the ramada eastgate in july I had to change rooms because the room smelled awful. the rug was wet amd moldy so it smelled . the grounds were ok xcept for the swamp they called a lake with the broken gazebo that had caution tape on it . I cant speak for the food I didnt even try it the restaurant had a buffet style seafood dinner for 20.00 pp the shuttle buses to disney only made 3 pickups so you had to go into the parks by 11 45 am or you werent going unless you called a cab . the staff was friendly but the rooms need a totlal renovation from the dingy shower curtains to the cigarette burned linens it was not even standard in my book i am a seasoned traveler and i will never stay at a ramada again. go with the competetion like holiday inn or travelodge i met some people from there and they had NO complaints
Took one look and walked out
We had booked into this hotel as we had stayed there a few years before and it was fine. However, on this occassion we pulled into the car park and were gobsmacked when we saw the state of the place. There was rust running down the outside balconies and the hotel looked like a 'Batesy's' Motel!We thought maybe it would be better on the inside so went inside to look only to find the restaraunt packed full of people who looked like they were auditioning for a Jerry Springer show!The man on the front desk was unhelpful and scruffy. The floor tiles in reception were all loose and rocked back and forth when you stood on them.We decided to venture no further and got out of there as quick as we could, even though we had paid for our accomodation up front and would lose the money paid.
TripAdvisor Reviews Ramada Inn Resort Eastgate Kissimmee
Travel Blogs from Kissimmee
We have had an absolutely amazing time in Orlando! We started by having 3 days in Universal riding all the awesome roller coasters. Our favourite rides were the hulk, rock it, the dragons and gringotts! We experienced Harry Potter world whilst we were there which was amazing! The amount of effort that has gone into making it look real is incredible. We also went to watch the blue man group perform and got VIP passes to ...
The Land of Mickey
... the wait time sign said 45 minutes but people leaving the ride said it was less than 10 minutes. Ted and I watched Wendy while the rest ran on in. Reese said this was his favorite ride of the park. Now with everyone happy we walked the long walk back to the monorail, the wait for the trams ant the the walk to Sparky. We got everyone settled in the back for the 45 minute ride back to the condo. We had a marvelous day with happy well behaved children.
Last minute shopping....
... found more 'bargains' but I was living under this obvious misconception that most shops would be 'giving away' their wares, being an outlet mall. Anyway, it was incredibly hot again when we finally bade farewell to Orlando where we had had a great time and created some fantastic memories! We flew out on Lufthanza to London, via Frankfurt, a trip that was going to take approximately 18 hours ( including ...
Star Wars Day
... We went to an Indiana Jones Stunt Show which had Harrison Ford's stunt double as India and they did lots of stunts. We also went on Star Tours again for a different ride and then to Beauty and the Beast, a musical.
Then we took a bus back to the hotel and relaxed.
USA fact: There are a lot of people with mobility scooters at ...
Leisurely start - Epcot
After exploring the resort for a little while we headed to Epcot. The bus to Epcot arrived after about five minutes. Once there, we went to Club Cool to try out some of the new Coke flavors from around the world. Since we had time to spare before lunch, we decided to do something we've never done, O'Canada. It's a 15 minute 360° movie showing the beautiful ...


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this wiki
We are currently editing over 2,759 articles and 26,877 images with 291,472 edits and we want you to help!
Beyblade Burst
The next generation of Beyblade has arrived!
Beyblade: Shogun Steel
Experience the finale of the Metal Saga!
Classic Beyblade
Revisit the Original Series!
Learn about Beyblade!
About this Wiki | Statistics | Policy | New Pages | New Files | Categories | Wiki Tutorial | Help Pages | Disclaimer
Beyblade Wiki is a collaborative encyclopedia about the hobby and anime, Beyblade. It is kept up-to-date by editors like you, to make sure Beyblade Wiki becomes the largest database on Beyblade. Become a member and you can help us expand Beyblade so it may grow. Need an introduction to Beyblade Wiki, then go to the Community Portal. Be sure to read our Policies, as it provides the guidelines for the content posted. Also, use our tutorial for information on how to create, format, and edit pages. Read more...
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May 2016
- May 21st: B-44 Booster Holy Horusood .U.C is released.
- May 21st: B-45 Beyblade Light Launcher & Long Winder is released.
June 2016
- June 18th: B-46 Booster Obelisk Odin .T.X is released.
- June 18th: B-47 Weight Tamper is released.
July 2016
- July TBA: B-48 Starter Xeno Xcalibur .M.I is released.
- July TBA: B-49 Random Booster Vol. 3 Yeager Yggdrasil is released.
- July TBA: B-50 Burst BeyStadium Wide Type is released.
- July TBA: B-51 wbba. Finger Band is released.
- July TBA: B-52 BeyMisanga Maker is released.
- July TBA: B-53 BeyMisanga Victory Valkyrie Ver. is released.
- July TBA: B-54 BeyMisanga Storm Spriggan Ver. is released.
- July TBA: B-55 BeyMisanga String Set is released.
August 2016
- August TBA: B-56 Booster Unlock Unicorn is released.
- August TBA: B-57 Beyblade Deck Set is released.
- August TBA: B-58 Karabiner Grip (Burst) is released.
September 2016
- September TBA: B-59 Starter Zillion Zeus is released.
- September TBA: B-60 Metal Weight is released.
Late 2016
- Beyblade Burst begins release in North America.
Early 2017
- Beyblade Burst begins release in western countries.
|B016: Surprise! The Xhakuenji Special!!
|ZG045: The Bridge to the Future
|"As the battle between Zyro and Kira rages on, an interference from Doji puts them in a dangerous situation! Meanwhile, Gingka attempts to break free and take on Doji, who has transformed himself into an enormous Beyblade!"
Read more...
|4D052: A Ray of Hope
|"The final battle between Gingka Hagane of the Legendary Bladers and the God of Destruction, Nemesis continues! Pegasus, who is put in a tough position by Nemesis' barrage of attacks, holds it's ground using the spirits of it's fallen allies. At that moment, the wishes of Bladers across the world begin to cluster within Gingka's body..."
Read more...
|GR051: Beybattle for the Ages
|"Brooklyn, who has been corrupted by his inner depression, takes his battle with Tyson towards the skies in an attempt to isolate himself from everyone else. Tyson, who realises he cannot defeat Brookyln without first consoling him and showing him a fun battle with friends, follows Dragoon above the clouds to find Brooklyn. Using the wishes of both his and Brooklyn's loyal friends, can Tyson put Brookyln out of his destructive misery? "
Read more...
|#04: Chapter 04
|"Having reached the Semi-Finals of the tournament, Valto and Shu decide to train together to prepare themselves for their opponents. Valto's opponent is Wakiya Komurasaki, who uses the Defense-type Wyvern Armed Massive. Can Valto advance to the finals where Shu is waiting for him?"
Read more...
|#09: Beyblade Forever!
|"Zyro's battle to thwart Kira's attempt at total global destruction continues. All hope rests with the Synchrome of his Samurai Ifrit W145CF and Shinobu's Shinobi Salamander SW145SD, the Salamander Ifrit W145CF. Motivated by his dream of meeting Gingka Hagane, Zyro presses onward in his decisive battle against Kira..."
Read more...
Coming soon...
Coming soon...
| Beyblade Wiki Network
Summer Season 2016 Anime Wikis
All • Action • Adventure • Comedy • Drama • Fantasy • Horror • Mecha • Mystery • Romance • Sci-fi • Slice of Life • Sports • Supernatural • Memes • Funimation
|Member of Wikia: Toys and Games
Wikia: Toys and Games (TaG) is a family of open-content encyclopedias based on toy/game franchises, vowing to help each-other, wherever help is needed, and create a family of unified wikis.


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Via MillerOutdoorTheatre:
An Evening with War Live FREE at Miller Outdoor Theatre, Saturday July 6, 2013 at 8:30pm. Sounds like the perfect date night to me. Click here for more details
Also On Majic 102.1:
Like Father, Like Son: 7 Times Denzel Washington's Son John David Stole Our Heart
10 photos Launch gallery


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|Jul 30 22:33 GMT
Forex Expos
Fundamental Analysis Reports
Weekly Economic and Financial Commentary
Jul 30 04:25 GMT, by
Economic activity improved slightly in the second quarter to 1.2 percent lifted by a surge in consumer spending. Meanwhile, the Federal Open Market Committee (FOMC) left the fed funds target range un...
The Weekly Bottom Line
Jul 30 04:06 GMT, by
Appropriately for the dog days of summer, there was plenty for markets to chew on this week. U.S. economic news was generally mixed. And, a highly anticipated Bank of Japan decision ultimately fell s...
Market Review & Outlook: Central Bank Parade Marches On
Jul 30 03:56 GMT, by
The past week has seen heightened market volatility surrounding monetary policy decisions from major central banks, along with a deluge of company results in the thick of earnings season. The US Fede...
Week Ahead Central Bank Stimulus in Focus
Jul 30 03:53 GMT, by
The U.S. Federal Reserve and the Bank of Japan (BOJ) showed the two sides of central bank action this week. The Fed's Federal Open Market Committee (FOMC) ended with no change to the U.S. benchmark i...
Second Quarter Growth Disappoints Despite Consumption Strength
Jul 29 13:47 GMT, by
Real GDP increased by an annualized 1.2% in the second quarter of 2016 according to the advance BEA estimate, or about a half of the expected growth rate of 2.5%. To make matters worse, growth in the...
Bank of Japan Eases Less than ExpectedAll Daily Fundamental Reports >>
Jul 29 13:44 GMT, by
Yesterday the Bank of Japan (BoJ) decided to ease monetary policy by almost doubling its annual purchases of ETFs to 6 trillion yen from 3.3 trillion yen. In addition, the bank doubled its U.S. dolla...
Technical Analysis Reports
EUR/USD Appproaching 1.11 Level On Friday
Jul 29 08:13 GMT, by
The common European currency surged on Thursday to 1.1076 by the end of day's trading session. In addition, it was more volatile during the session to the upside, as the pair touched the 1.1120 level...
GBP/USD Risks Suffering More Losses
Jul 29 08:12 GMT, by
Yesterday the Cable behaved according to expectations, as the immediate support cluster succeeded in limiting the losses. Technically, the GBP/USD pair should now rebound and end the week with anothe...
USD/JPY Falls After BoJ Fails To Deliver
Jul 29 08:11 GMT, by
The US Dollar remained relatively unchanged against the Japanese Yen on Thursday, as a calm before the storm. And the storm did deliver, the USD/JPY currency pair plunged over 250 pips after the BoJ ...
Gold Continues To Fluctuate Around 1,340 Level On Friday
Jul 29 08:08 GMT, by
As it was stated yesterday, all what the FOMC announcement achieved was a movement of gold price fluctuation by 20 US dollars northwards. On Friday morning, the yellow metal continues to fluctuate ar...
Forex Technical Analysis
Jul 29 07:46 GMT, by
EUR/USD Current pullback after 1.1120 peak should be considered corrective, preceding another rise towards 1.1190 resistance area. Key support lies at 1.1030-40.
Wave Analysis Clarify Extensive Correction Triangles In ForexAll Daily Technical Reports >>
Jul 29 06:44 GMT, by
The EUR/USD continues its long-term consolidation pattern by staying between support (green) and resistance (orange). A bearish breakout is needed before the wave count is confirmed whereas any move ...
Analysis Report Search
Long Term Forecasts
WTI Crude Oil A Year Ahead - Trends And Expectations 2016
Dec 24 11:45 GMT, by
The second half of 2014 saw a sharp drop in the price of WTI Crude Oil, which had traded at $105 a ...
DAX Year Ahead – Trends And Expectations 2016
Dec 24 11:11 GMT, by
It has been another testing year for the German economy but once again it has shown the kind of str...
British Pound Year Ahead - Brexit To Dominate 2016
Dec 22 12:49 GMT, by
This has been something of a turnaround year for the UK in which unemployment has fallen back to pr...
Gold for Bear Market?
Oct 13 13:23 GMT, by
A "bear market" is frequently defined as a decline of at least 20% in the S&P 500 index. Troubl...
Euro Area Outlook for 2015: Impact of Broad-Based QEAll Long Term Forecasts >>
Jan 17 03:46 GMT, by
The expected ECB QE programme will support economic activity through a number of channels and hence...
|ActionForex.com © 2016 All rights reserved.


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Jim Cramer broke down what he thinks is the real story behind the Verizon and Yahoo deal. » Read More
There's no question that journalism needs a new business model. But until now, no one has come up with the solution. Three veterans of print publications are teaming up to launch their solution to the media-monetization problem.
With stocks rallying for 5 weeks in a row now, dividend yields are falling back to Earth. The average dividend yield of the Dow 30 has fallen over 25% since the rally began in early March. See how the 30 companies in the Dow compare.
From the "Obama effect" to a rebound in consumer confidence, Research in Motion is tapping into several trends besides the technology inherent in its products in order to drive its stronger-than-expected earnings performance.
The Dow rallied for a third day on Thursday, and traded above 8,000 for the first time since early February on an intra-day basis.
Individual performance doesn't seem to matter when it comes to manager salaries. It's all about what the next guy makes.
Even the business world's worst executives are making big money. So much for performance-based compensation.
These companies will tell us whether or not Monday's rally was real.
Here is a breakdown of where the jobs are right now by sector. See what companies are hiring what types of jobs and in which states. Click through to see all major sectors.
Cramer makes the call on viewers' favorite stocks.
Tuesday: Rep. Barney Frank (D-Mass.) said the uptick rule will soon be reinstated but the SEC said that mark-to-market regulations would remain in place. Citigroup shares skyrocketed nearly 40 percent and many other financials followed suit — lifting the market with them. General Electric* shares enjoyed an assurance from Citi and AT&T said it'd add — yes, add — 3,000 jobs and invest up to $18 billion. CNBC heard from experts who said that investors are nearly ready to get out of cash — and gave a prescription for bear repellant.
Stocks declined Wednesday as a late warning from President Obama about stricter oversight for Wall Street knocked major indexes off their highs for the day.
Is there such a thing as a safe dividend yield? RNC Genter Capital Management's Dan Genter says an investor should look not to the size of the dividend, not even in relation to the stock price, but to consistency.
Following its second best day of the year yesterday, the markets are poised for a slightly lower open, with little reaction to President Obama's first speech to a joint session of Congress.
US stocks looked set to give back some the gains from the previous session at the open Wednesday, after Federal Reserve Chief Ben Bernanke soothed investors by stepping back from bank nationalization plans and saying the recession may end this year.
Cramer makes the call on viewers' favorite stocks.
The Dow and S&P slumped to 11-year lows on Monday as investors lost faith that the U.S. government will be able to stabilize the financial system.
Stocks fell flat as investors grew more confident that the government will stabilize the battered financial sector, but technology remained weak.
US stock index futures pointed to a higher open Monday as shares of Citigroup soared over 25 percent in pre-market trading, following reports the government could up its stake in the Wall Street giant.
Investing is a Darwinian death match these days. Here's how you live through it.
Looking for a formula for successful stock investing? RBC Bank's Joseph Keating sums it up in three words: exceptionally high quality. Companies in that category, he says, are likeliest to hold onto their dividends. (Part One)


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Skip to Main Content
IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards,
eBooks, and eLearning courses.
Learn more about:
IEEE membership
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With the spread of information home appliances certified by Digital Living Network Alliance (DLNA), it has become quite easy to share digital contents within a home network. In the meantime, the mobile Internet has been drawing much attention along with the popularization of high-performance mobile devices and the development of high-speed wireless communication technologies. Under such circumstances, there is a growing desire to obtain digital contents stored in a home network from a remote network in a simplified manner. In this paper, we propose a DLNA-based, remote communication method that enables the sharing of contents between a home network and a foreign network, by expanding functions of NTMobile (Network Traversal with Mobility), which is our original technology that can realize mobility and solve the NAT traversal simultaneously. By implementing our proposed method in a prototype system, we confirmed that a node can transmit media contents over a NAT and keep on browsing the contents even when the node moving to another network during communication.
A not-for-profit organization, IEEE is the world's largest technical professional organization dedicated to advancing technology for the benefit of humanity.© Copyright 2016 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.


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Get Back to Nature Stage
New for 2016 is the Get Back to Nature Stage sponsored by Ready Camp.
Pick up great ideas for exploring the best of the British countryside in your caravan, motorhome or camping with the family on this brand new feature. There will be a host of fascinating talks from celebrity speakers including TV Presenter and Marine Adventurer Monty Halls, as well as presentations by experts highlighting the best bird watching spots, cycling routes and walking trails in the UK. Click here to see who will be speaking at the show.
There's plenty to get the kids involved too! Forest Rangers will be on hand uncovering the magic of woodland; the kids will discover animals, birds and bugs as well as how to pitch a tent and what's involved in doing their Duke of Edinburgh Award!
Discover an exciting line-up of expert speakers and interactive talks which will get you raring to put your wellies on and get exploring the beautiful British countryside.
Ready Camp is the Camping and Caravanning Club's new glamping option, available at a network of 17 different locations across the UK. Stay in a pre-erected safari style tent, complete with real beds and a relaxing sofa – a unique camping experience, and all you need to bring is the bedding.


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Many Angelenos have blissfully allowed the outside world to think of our city as overrated, but I'm going to let you in on a little secret: it's pretty... Read More
After the success of the Hello Kitty truck, which cruised Los Angeles during the Hello Kitty convention last year (yes, that's a thing), parent company... Read More
One thing we can all relate to is that sinking feeling that comes when you arrive at the airport before a long flight only to be greeted by hordes of... Read More
Life—and especially vacation—is too short to spend on time-wasters. San Francisco, though known for its creative culture, is not immune to having its... Read More
Black Friday is historically a day of pushing and shoving and haggling. It's called "black," for a reason. All of that scrambling for stuff makes our... Read More
In honor of its Diamond Anniversary, L.A.'s Beverly Hilton is offering a package with the precious sparkler as inspiration. Partakers begin... Read More
Federal security authorities at the Los Angeles International Airport today seized 450 pork tamales from one traveler's luggage. The man, coming back... Read More
Sanrio—Japanese toy company, creator of Hello Kitty, and purveyor of all things cute—has teamed up with Plan Check, L.A.'s popular burger-and... Read More
Los Angeles is a world-class shopping destination—the first U.S. city to have flagship stores for Neapolitan tailoring masters Isaia, and COS, the... Read More
Wanting to travel is never the problem; finding the time and the money often can be, though. But in these days of flash sales and incentives,... Read More
You're in San Francisco for Thanksgiving this year and have a free day to roam the city. Since you decided against joining the Black Friday mobs in... Read More
It might seem near impossible to enjoy a weekend in a major metropolitan area like Los Angeles on a budget, but it can be done—especially if you opt for... Read More
Ignore those depressing headlines about how expensive San Francisco has become (both for renters and travelers)—there's still plenty to do on a budget... Read More
A mysterious light in the night sky shocked San Diego residents and led many to call law enforcement out of safety concerns. Now, the U.S. Navy says a... Read More
San Francisco doesn't need another shop stuffed to the gills with midcentury modern, and thanks to Jonathan Rachman, it's not getting one right now. The... Read More
San Diego: where the beer flows like water. Perhaps better known for its beautiful beaches, deliciously ubiquitous Mexican food, and pristine year-round... Read More
After running an eight million dollar campaign in the San Francisco city election, Airbnb has emerged victorious, winning 55 percent of votes in the... Read More
The San Diego International Airport stopped all landings after police reported an active shooter in the city's Little Italy neighborhood. ... Read More
San Francisco is suffering from a housing crisis that has propelled rents into the stratosphere. And according to the supporters of a ballot measure to... Read More
Over the last few years, San Francisco has developed a bit of a crush on gin. A number of small batch artisanal distilleries have opened around the Bay... Read More
Tucked into St. Helena's serpentine Silverado Trail, in a tangled grove of knotty oaks and pine trees, out of view of the limos and luxury wine-tasting... Read More
In San Francisco, tourists celebrate Halloween; locals celebrate Dia de los Muertos, Mexico's "Day of the Dead." The holiday dedicated to honoring the... Read More
Reality check: the ubiquitous pumpkin spice latte is not the only drink that signals fall. Especially not in San Francisco, where the bartenders are... Read More
As of this week, the Bay Area boasts a brand new three-Michelin-starred restaurant. Michelin announced on Tuesday that Manresa, the Los Gatos restaurant... Read More


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I was about to start customising the CSS for the admin myself when I thought I cannot be the only one who is frustrated by the lack of contrast within the WordPress admin.
The plugin works as it says. Even if you have 20/20 vision you will struggle to view the inputs and options within WordPress. I cannot believe the core team thinks this is acceptable. My vision is excellent but my new LED monitor is extremely bright which makes the subtle shades of grey and white indistinguishable. If I tweak the contrast to suit this the colours suffer. Even in a well lit room you can struggle to view the admin boxes.
Thanks very much for wrapping this up in a plugin.
I'd love to see some options in the future for a high contrast version (for DDA, etc.) but maybe there is another plugin out there already.
My eyes finally rest :-) Thank you !
Does exactly what it says on the tin :)
You feel like you got glasses
You must log in to submit a review. You can also log in or register using the form near the top of this page.


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Candlewood Suites San Antonio Downtown
Candlewood Suites San Antonio Downtown
Quick Info
- Check In: 3:00 PM
- Check Out: 12:00 PM
- Minimum Check-In Age: 21
- Late Check Out Available
- Parking: Free
- Print Fact Sheet
- Chat With Us
Important Update
- On-site Guest Self-Laundry Facilities
- Standard Wireless Internet service is free for all members
- On-site Fitness Center Available
- Outdoor Gazebo Grill
It's hard to leave your best buddy at home when you're traveling for extended periods, so we provide a pet friendly experience. Restrictions and fees apply.
Whether you are booking suites for a family reunion or suites for your company's training needs, Candlewood Suites hotels can accommodate your group.
Workout whenever you want: Candlewood Gym always has an open door and lots of aerobic and cardio equipment to make it easier to stick to your exercise routine, no sweat.
The IHG Green Engage system is an online tool that gives our hotels the means to measure their day-to-day environmental impact.
Getting Here
Guest Reviews
Overall Experience
July 25, 2016
Great coffee, comfortable beds, good service good location near downtown. Good attention to detail,
need a little customer 101!
Great Stay
July 19, 2016
I could not say enough great things about the staff!!! I enjoyed myself so very much and felt like family. I was there for 94 nights and the staff made me feel at home. Anytime I had a question or a need it was handled immediately. So many positives that it's hard to believe… Thank you Candlewood for such a great experience so far away from home…It truly felt like leaving family on the day I checked out.
gREAT FOR AN OVERNIGHTER
July 17, 2016
Well located and consistent with the best of Candlewoods, particularly liked the Candlewood Cupboard giving single traveller more flexibility. Very friendly staff
Pleasantly Surprised
June 20, 2016
We had a pleasant experience during our stay. The check in was simple and staff was very welcoming. The room was comfortable and at a good temperature. We spent most of our time at the River Walk. I wouldn't recommend walking from the hotel, but it's a quick 5 min. drive.


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Publisher: CollinsPages: 64
Snuffler is the youngest, and the only boy. His sisters think he is horrible, greedy and spoilt and needs to be taught a lesson - unfortunately for the girls he is also very clever! Here are 2 stories about the Gussie family of guinea pigs.
*A minimum purchase of $35 is required. Shipping is provided via FedEx SmartPost®
and FedEx Express Saver®. Average delivery time is 1 – 5 business days, but
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Bare Escentuals bareMinerals Well-Rested Face & Eye Brightener
13 reviews
23% would repurchase
Package Quality: 2.8
Price: $$
Package Quality: 2.8
Price: $$
on 5/15/2016 9:54:00 PM
Age: 19-24
Skin: Combination, Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
It doesn't conceal my under eyes at all or highlight. Maybe I'll just use it a base before I apply my powder highlight. Sadly, this product does not do what it claims to, the concealer isn't opaque at all.
on 1/8/2016 3:35:00 PM
Age: 44-55
Skin: Dry, Fair, Cool
Hair: Blond, Wavy, Fine
Eyes: Green
This is a sheer liquid highlighter. It does not have coverage if you need to hide dark circles; however, I absolutely love it and find that it really does make me look well-rested. I use it after I apply my eye-makeup and on top of foundation. If you need to conceal dark circles, use it on top of your concealer. It can dry very quickly so I pat it on my fingers first and then pat underneath my eyes and out across the tops of my cheekbones. If my skin is feeling dry, I'll add the tiniest drop of olive oil to the highlighter in my hand before I apply. It's almost miraculous on me. The one thing I can't stand is the packaging. It has a very tight-fitting, slick cap and if there is any makeup or moisturizer on my hands, which there usually is since I'm applying makeup, I can't get the lid off.
To Read all 13 Reviews, Login or Join
on 11/10/2015 1:57:00 PM
Age: 56 & Over
Skin: Dry, Fair-Medium, Not Sure
Hair: Brown, Other, Other
Eyes: Blue
I previously used well rested powder formula, but this creamy stick formulation is fabulous! It does the job and does not accentuate my wrinkles. I am over 60.
on 7/6/2015 7:06:00 PM
Age: 25-29
Skin: Acne-prone, Olive, Neutral
Hair: Other, Wavy, Coarse
Eyes: Hazel
review of: BE mineral powder concealer in the shade well-rested:
I returned this $18 product because:
Highlighted under eye bags: resulted in looking like I had 2 glowy, but fading black eyes!
Cap did not click shut right out of the bag.
I briefly tried this as an all over redness reducer and it worked better for that purpose. However, I found this product worked best as a matte, slightly banana-colored highlight on the brow bone and cupids bow. Similar to the UD shade foxy but without talc. $18 isn't terrible but it didn't deliver what the product description promised. Decent ingredients and spf 20 earned this an extra lippie. Would never recommend
on 7/5/2015 10:25:00 PM
Age: 18 & Under
Skin: Normal, Fair-Medium, Cool
Hair: Brunette, Straight, Fine
Eyes: Green
This is terrible. Did not do anything for dark circles.
on 4/6/2015 10:43:00 AM
Age: 36-43
Skin: Very Oily, Olive, Warm
Hair: Brunette, Straight, Coarse
Eyes: Brown
***Highlighter Pen Formula Review ***
I got the BE Stroke of Light (see review) which I disliked and decided to try this one out too. I'm currently looking for one of those highlighter/brightener pens. In the past, I've tried YSL, Dior, Maybelline and L'Oreal which all failed to meet my expectations. This was interesting because of the shade. It is straight up white with tons of micro glitter. Also, the name "well rested" intrigued me. I wanted to look awake but not necessarily want a product that was a concealer. I already own great concealers. I wanted simply a brightening effect for shadows of the face. It retails for $22 for 0.10 fl. oz. The packaging is nice. It's small and slim with a brush top. The brush tip is soft and comfortable to use. The packaging is all I cared for with this highlighter.
The Cons: Shade, formula, performance and effect. The shade along with the heavy micro glitter is very unnatural on the skin. It just turns your skin into fish scales. The formula is extremely thin and with the first strike of the pen, you see no color but a line of glitter. The effect is as though a magical snail rolled along your face. Really not the look I was going for.
on 3/27/2015 2:02:00 PM
Age: 30-35
Skin: Combination, Fair, Cool
Hair: Red, Wavy, Fine
Eyes: Green
This came in a set I bought. At first it seemed really useless. It doesn't provide coverage. It doesn't work very well as a highlighter. It's just sort of clear and sticky with a little shimmer. However, I was hoping to find some way to use it. So what I've found works well is to put it on my undereye area, toward the inner corners mostly, and then use my concealer. I have darkness there, and also dry skin, and this winds up providing a really nice base for my under eye concealer. It brightens just a touch, and also seems to help a bit to keep my undereye concealer drying out completely and fading off.
on 11/29/2014 7:16:00 AM
Age: 30-35
Skin: Dry, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
I'll preface this review by saying that I've never been a huge fan of Bare Escentuals. I've tried it so many times over the years and it just doesn't work for me. That being said, I do like some of their products so I was super excited to try the Well-Rested as I'd been on the hunt for the perfect natural looking highlight and according to all of the online and YouTube reviews, this was a hit. Yet again, this mineral powder just didn't do it for me and there are a few reasons why: 1) I'm light to medium skin-toned and this yellow-toned corrector made me look as if I had sat in the sun all day with a pair of sunglasses on; 2) It sat on top of my skin, regardless of how much I tried to buff it in, it just sat there and was very apparent, even 4 hours after application; 3) One tap of product into the lid was one tap too much. It's very hard to control quantity use.
Keep in mind that a lot of people love this brand and it works really well for them, so you might be happy with this, it just wasn't for me. I have relatively dry skin, and it's very sensitive so although this powder product is somewhat creamy, it was still drying and not as blendable as I would have liked.
on 8/29/2014 11:02:00 PM
Age: 25-29
Skin: Acne-prone, Fair, Warm
Hair: Brunette, Wavy, Medium
Eyes: Green
I was surprised at how much I enjoyed this. It came in a kit I bought and I used it since I had it. Brightened up my under eyes nicely to make me look more awake. Miss this product now that I've run out but not planning to rebut as it's not necessary to me.
on 8/29/2014 12:06:00 AM
Age: 30-35
Skin: Very Oily, Medium, Neutral
Hair: Brunette, Straight, Fine
Eyes: Brown
I was excited to try this as many people like the well rested powder, which I find a bit light for me. This was not any better. In fact it was too sheer for my under-eye and also looked unnatural. I am not a fan of any of the Bare Escentuals and I think this will be my last product I try from them. I always keep trying it expecting something new to happen but they are always a fail and an expensive one at that.
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