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The mammalian circadian timing system consists of a master pacemaker in neurons of the suprachiasmatic nucleus (SCN) and clocks of a similar molecular makeup in most peripheral body cells. Peripheral oscillators are self-sustained and cell autonomous, but they have to be synchronized by the SCN to ensure phase coherence within the organism. In principle, the rhythmic expression of genes in peripheral organs could thus be driven not only by local oscillators, but also by circadian systemic signals. To discriminate between these mechanisms, we engineered a mouse strain with a conditionally active liver clock, in which REV-ERBα represses the transcription of the essential core clock gene Bmal1 in a doxycycline-dependent manner. We examined circadian liver gene expression genome-wide in mice in which hepatocyte oscillators were either running or arrested, and found that the rhythmic transcription of most genes depended on functional hepatocyte clocks. However, we discovered 31 genes, including the core clock gene mPer2, whose expression oscillated robustly irrespective of whether the liver clock was running or not. By contrast, in liver explants cultured in vitro, circadian cycles of mPer2::luciferase bioluminescence could only be observed when hepatocyte oscillators were operational. Hence, the circadian cycles observed in the liver of intact animals without functional hepatocyte oscillators were likely generated by systemic signals. The finding that rhythmic mPer2 expression can be driven by both systemic cues and local oscillators suggests a plausible mechanism for the phase entrainment of subsidiary clocks in peripheral organs.
Author Summary
In contrast to previously held belief, molecular circadian oscillators are not restricted to specialized pacemaker tissues, such as the brain's suprachiasmatic nucleus (SCN), but exist in virtually all body cells. Although the circadian clocks operative in peripheral cell types are as robust as those residing in SCN neurons, they quickly become desynchronized in vitro due to variations in period length. Hence, in intact animals, the phase coherence between peripheral oscillators must be established by daily signals generated by the SCN master clock. Although the hierarchy between master and slave oscillators is now well established, the respective roles of these clocks in governing the circadian transcription program in a given organ have never been examined. In principle, the circadian expression of genes in a peripheral tissue could be driven either by cyclic systemic cues, by peripheral oscillators, or by both. In order to discriminate between genes regulated by local oscillators and systemic cues in liver, we generated mice in which hepatocyte clocks can be turned on and off at will. These studies suggest that 90% of the circadian transcription program in the liver is abolished or strongly attenuated when hepatocyte clocks are turned off, indicating that the expression of most circadian liver genes is orchestrated by local cellular clocks. The remaining 10% of cyclically expressed liver genes continue to be transcribed in a robustly circadian fashion in the absence of functional hepatocyte oscillators. These genes, which unexpectedly include the bona fide clock gene mPer2, must therefore be regulated by oscillating systemic signals, such as hormones, metabolites, or body temperature. Although temperature rhythms display only modest amplitudes, they appear to play a significant role in the phase entrainment of mPer2 transcription.
Citation: Kornmann B, Schaad O, Bujard H, Takahashi JS, Schibler U (2007) System-Driven and Oscillator-Dependent Circadian Transcription in Mice with a Conditionally Active Liver Clock. PLoS Biol 5(2): e34. doi:10.1371/journal.pbio.0050034
Academic Editor: Steve O'Rahilly, University of Cambridge, United Kingdom
Received: August 3, 2006; Accepted: December 1, 2006; Published: January 30, 2007
Copyright: © 2007 Kornmann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was supported by the Swiss National Science Foundation (through an individual research grant to US and the National Center of Competence in Research program Frontiers in Genetics), the State of Geneva, the Louis Jeantet Foundation of Medicine, the Bonizzi-Theler Stiftung, and the 6th European Framework Project EUCLOCK. JST is an Investigator at the Howard Hughes Medical Institute.
Competing interests: The authors have declared that no competing interests exist.
Abbreviations: bp, base pair; CAMKII, calcium/calmodulin-dependent kinase II; Dox, doxycycline; HSE., Heat-shock element; HSF, heat-shock transcription factor; kb, kilobase; SCN, suprachiasmatic nucleus; TRE, tetracycline-responsive element; tTA, tetracycline-dependent transactivator; WT, wild-type
In mammals, virtually all body cells possess self-sustained, cell-autonomous circadian clocks [1–3]. The oscillators in peripheral organs are entrained by a master pacemaker residing in the suprachiasmatic nucleus (SCN) of the brain's hypothalamus, which is itself synchronized by daily light–dark cycles [4]. The molecular details of the signaling pathways used by the SCN to phase-entrain peripheral clocks are still obscure; however, daily feeding–fasting cycles, circadian hormones, and body temperature appear to play pivotal roles in this process [5–9]. The accumulation of mPER1 and/or mPER2, two integral clock components, is altered upon the administration of phase-shifting cues. Hence, these proteins are likely to be involved in the synchronization of circadian clocks [10,11].
On the molecular level, mammalian circadian oscillators are thought to rely on two interconnected negative loops of clock gene expression [12,13]. According to this model, the principal feedback loop is driven by the repressors PER1, PER2, CRY1, and CRY2 and the PAS-domain basic helix-loop-helix (PAS-bHLH) transcription factors BMAL1, CLOCK, and probably NPAS2 [14]. The transcription of the repressor-encoding genes is activated by these PAS-bHLH transcription factors until the PER-CRY complexes reach critical concentrations at which they annul the transactivation potential of the PAS-bHLH proteins and thereby inhibit transcription of their own genes. The concentration of PAS-bHLH activators is adjusted by an accessory feedback loop in which the orphan nuclear receptor REV-ERBα (and, probably to a lesser extent, its paralog REV-ERBβ) periodically represses Bmal1 transcription. The inhibitory activity of REV-ERBα counteracts the transactivation activity of ROR nuclear orphan receptors, which bind to the same RORE elements within the Bmal1 promoter [15]. The cyclic expression of REV-ERBα is itself governed by the PAS-bHLH activators and CRY-PER repressors of the principal negative feedback loop, thereby interconnecting the Rev-erbα-Bmal1 feedback loop directly to the principal feedback loop [16,17]. Since BMAL1 and CLOCK are metabolically more stable than CRY and PER proteins, their abundance varies only slightly throughout the day [16,18,19].
Post-translational protein modifications are also believed to play important roles in the modulation of PER and CRY activities [18,20,21]. However, to date, Bmal1 is the only known clock gene whose inactivation immediately leads to arrhythmicity of behavior and to the ablation of mPer1 and mPer2 mRNA accumulation cycles in the SCN [22].
Transcriptome profiling studies have uncovered a large number of cyclically expressed genes [23–27]. Although most of these genes appear to be involved in output functions, some may also serve as input regulators participating in the synchronization of local clocks. The oscillating activity of these latter genes would be expected to integrate systemic cues such as circadian hormones, metabolites, or body temperature rhythms, into the clockwork circuitry of peripheral cell types. In the intact organism, the cyclic expression of such genes should not necessarily depend upon functional local clocks.
In this study, we describe our attempt to engineer a mouse strain with conditionally active hepatocyte circadian clocks. We used these mice to classify mRNAs into transcripts whose circadian accumulation in the liver do or do not require local circadian oscillators. The identification of systemically driven and oscillator-driven genes not only provides insight into the structural organization of the mammalian circadian timing system, but should also open new avenues to study the phase entrainment mechanisms of peripheral clocks.
The Construction of Mice with a Conditionally Active, Liver-Specific Rev-erbα Transgene
We wished to engineer a mouse strain with conditionally active circadian oscillators specifically in hepatocytes, in order to examine the contribution of local clocks and systemic Zeitgeber cues to rhythmic liver gene expression. As mentioned above, BMAL1 is a constituent of the molecular oscillator whose loss of function immediately results in the abolishment of all manifestations of circadian physiology and gene expression [22]. We thus thought that the conditional expression of Bmal1 specifically in hepatocytes may provide such a model system. Bmal1 transcription follows a high-amplitude circadian cycle, owing to the circadian accumulation of REV-ERBα, a nuclear orphan receptor that strongly represses Bmal1 transcription (Figure 1A) [16]. We thus exploited this regulatory mechanism to produce a mouse strain with a conditionally active Bmal1 gene in hepatocytes. First, a transgenic mouse strain was established in which transcription of an HA epitope-tagged REV-ERBα version (HA-REV-ERBα) is controlled by tetracycline-responsive elements (TREs). These mice were crossed with LAP-tTA mice, which express a tetracycline-dependent transactivator (tTA) specifically in hepatocytes [28]. In LAP-tTA/TRE-Rev-erbα double transgenic mice, HA-REV-ERBα accumulated to constitutively high levels and suppressed Bmal1 expression throughout the day in the absence of the tetracycline analog doxycycline (Dox) (Figures 1B, 1C [left panel], and S1). LAP-tTA/TRE-Rev-erbα mice thus produced HA-REV-Erbα in a liver-specific and tetracycline-dependent fashion. In the presence of Dox, the TRE-Rev-erbα transgene remained silent, and circadian oscillator function was not perturbed in liver cells (Figures 1B, 1C [right panel], and S1). As shown in Figure 1B, the regulation of TRE-Rev-erbα transgene expression was exquisitely tight, since in Dox-fed mice, neither mRNA nor HA-REV-ERBα protein was detectable by Taqman RT-PCR assays and Western blot experiments, respectively. At least in part, the low levels of Bmal1 mRNA and protein observed in the liver of untreated animals may have been contributed by endothelial cells, bile duct cells, or Kupffer cells, which did not express the LAP-tTA transgene [28]. In rat liver, nonparenchymal cells contribute about 35% of all cells and about 10% of the cellular hepatic volume [29,30], and we thus expect that around 10% of the liver RNA is contributed by cells that do not express the LAP-tTA transgene.
(A) Hepatocyte-specific, Dox-dependent expression of HA-REV-ERBα was achieved by placing a 5′-HA-tagged REV-ERBα cDNA transgene under the control of seven TREs (Tet-responsive elements). In the liver of mice expressing the tetracycline (Tet)-responsive transactivator (tTA) from the hepatocyte-specific C/ebpβ-LAP locus control region, HA-Rev-erbα transcription is constitutively repressed in the absence of the tetracycline analog Dox (tet-off system). This leads to an attenuation of circadian oscillator function, since Bmal1 is required for circadian rhythm generation.
(B) LAP-tTA/TRE-Rev-erbα double transgenic mice display high HA-Rev-erbα mRNA and protein levels throughout the day in the absence of Dox (−Dox). In the presence 3 g/kg Dox in the food (+Dox), neither HA-Rev-erbα mRNA nor protein can be detected.
(C) The levels of both Bmal1 mRNA and BMAL1 protein are dramatically down-regulated in the absence of Dox (compare the lanes on the left to those on the right).
We have used mice homozygous for both the LAP-tTA and TRE-Rev-erbα transgenes in these experiments, in order to maximize transgene expression. Double homozygous mice were born at Mendelian ratios and did not show any phenotype with regard to morphology, vigor, litter size, or circadian behavior. We also determined the integration site for both transgenes (see Materials and Methods). LAP-tTA was found to be inserted in reverse orientation into the first intron of Zfp353 (Chromosome 8), more than 100 kilobases (kb) downstream of the first exon and about 200 kb upstream of the second exon. TRE-Rev-erbα was found to be inserted in reverse orientation into the first intron of Semaphorin3ε (Chromosome 5), about 6 kb downstream of the first exon and more than 100 kb upstream of the second exon. We thus consider unlikely that the transgene integrations interfered with circadian clock function.
The Hepatic Expression of Putative BMAL1 Target Genes in Mice Fed with or without Dox
As would be expected for BMAL1 target genes, the expression of mPer1, Dbp, and endogenous Rev-erbα was low in the absence of Dox, when HA-REV-ERBα overexpression attenuated Bmal1 transcription. Unexpectedly, however, the circadian clock genes mCry1, mCry2, and mPer2 displayed milder expression differences in Dox-treated and untreated animals (Figure 2). Remarkably, the rhythmic expression of mPer2 mRNA and protein levels was almost unaffected by the down-regulation of Bmal1 expression. As reported previously [16], mCRY2 oscillated in abundance during the day despite nearly constant mCry2 mRNA levels. Conceivably, the association of mCRY2 with PER proteins—i.e., mPER2 in the absence of Dox—affected the metabolic stability of mCRY2 in a daytime-dependent manner.
(A) TaqMan real-time RT-PCR of cDNA was performed with liver whole-cell RNA for the transcripts of Dbp, endogenous Rev-erbα, mCry1, mCry2, mPer1, and mPer2 from untreated LAP-tTA/TRE-Rev-erbα mice (solid lines; −Dox) and Dox-treated LAP-tTA/TRE-Rev-erbα mice (dotted lines; +Dox).
(B) Western blot analysis of liver nuclear extracts from LAP-tTA/TRE-Rev-erbα mice that were fed with normal chow (−Dox) or Dox-treated chow (+Dox). In accordance with the temporal mRNA profiles shown in (A) and (B), mCRY1 and mPER1 display reduced levels in untreated mice, whereas mPer2 and mCry2 accumulate to similar levels in nuclei of Dox-treated and untreated animals. The varying mPER1 migration is probably due to oscillating protein phosphorylation and dephosphorylation (see [18]).
The robust circadian expression of mPer2 in the liver of mice not receiving Dox is in stark contrast to the in situ hybridization experiments with coronal brain sections of Bmal1-deficient mice, which indicated that in the absence of BMAL1, mPer2 mRNA accumulates to insignificant levels throughout the day in SCN neurons [22]. However, it is in keeping with the relatively high constitutive mPer2 mRNA concentrations observed in the liver of these Bmal1 knockout mice (J. S. Takahasi, unpublished data), assuming that in liver, mPer2 transcription depends less on BMAL1 than in the SCN. Nevertheless, our observation could be interpreted in two ways. Either, the residual BMAL1 levels in the liver of animals not treated with Dox were still sufficient to drive mPer2 transcription, or cyclic mPer2 expression was governed by oscillating systemic signals in these mice. In order to distinguish between these two scenarios, we wished to monitor temporal mPer2 expression in cultured liver explants, which obviously do not receive periodic signals from a master pacemaker. To this end, we crossed LAP-tTA/TRE-Rev-erbα mice with mPer2::luc knock-in mice [2], in which a luciferase open reading frame (ORF) was inserted by homologous recombination into the endogenous mPer2 locus. The mPER2::LUCIFERASE fusion protein encoded by this knock-in allele is fully functional, since it rescues all known rhythm phenotypes of mPer2 knockout mice [2]. Tissue explants from the LAP-tTA/TRE-Rev-erbα transgenic mice carrying an mPer2::luc fusion allele were placed into culture medium containing luciferin, and bioluminescence was recorded in real time by photomultiplier tubes [2,31]. As shown in Figure 3A (top right panel), liver explants from these mice did not produce circadian luminescence cycles in normal culture medium, suggesting that overexpression of HA-REV-ERBα indeed arrested the hepatocyte clocks. However, when tissue pieces from the same livers were cultured in Dox-containing medium (Figure 3A, right center and bottom panels), circadian luminescence rhythms similar to those observed for explants of mPer2::luciferase mice not carrying the LAP-tTA and TRE-Rev-erbα transgenes (Figure 3A, left panels) could be observed. Interestingly, circadian luminescence cycles recorded from Dox-treated liver explants of LAP-tTA/TRE-Rev-erbα/mPer2::luc mice fed with normal chow (Figure 3A, right center panel), displayed a phase delay of approximately 6 h when compared to those obtained from liver explants of mPer2::luciferase mice (Figure 3A, left center panel). This phase delay probably reflected the time period required for the decay of HA-Rev-erbα mRNA and protein, and for the consecutive accumulation of BMAL1 to levels compatible with circadian rhythm generation. In keeping with this conjecture, no significant phase differences were observed between luminescence cycles monitored for liver explants from mPer2::luc and LAP-tTA/TRE-Rev-erbα/mPer2::luc mice pretreated with Dox by intraperitoneal injections 48 h and 24 h before being sacrificed, (Figure 3A, bottom panels). We have examined liver explants from five mice homozygous (Figure 3A, and unpublished data) and three mice heterozygous (Figure S2A) for the LAP-tTA/TRE-Rev-erbα transgenes, and in all cases, circadian mPer2::luc expression strictly depended upon the addition of Dox to the culture medium. As expected, lung explants from either homozygous (Figure 3B) or heterozygous (Figure S2B) LAP-tTA/TRE-Rev-erbα/mPer2::luc mice displayed circadian luminescence rhythms, irrespective of whether or not Dox has been added to the culture medium. Indeed, TRE-Rev-erbα transgene expression is not detectable in this tissue by quantitative TaqMan real-time RT-PCR (unpublished data).
(A) Liver slices from mPer2::luc (left) and LAP-tTA/TRE-Rev-erbα/mPer2::luc (right) mice were cultured in luciferin-containing medium in the absence (−Dox) or presence (+Dox) of 10-ng/μl Dox. Luminescence was recorded using photomultiplier tubes. −Dox and +Dox samples are from the same animal; +Dox (pretreated) samples are from mice that have received two intraperitoneal injections of Dox 48 h and 24 h before being sacrificed.
(B) Lung explants from LAP-tTA/TRE-Rev-erbα/mPer2::luc mice were cultured as above in the presence or absence of Dox.
Taken together, our observations made with LAP-tTA/TRE-Rev-erbα mice and tissue explants suggest that in liver, circadian mPer2 expression can be driven by systemic Zeitgeber cues in the absence of functional hepatocyte clocks as well as by hepatocyte oscillators in the absence of systemic Zeitgeber cues.
Genome-Wide Mapping of Circadian Transcripts in Liver Cells with Operative or Attenuated Circadian Oscillators
To discriminate between oscillator-dependent and -independent circadian gene expression in a genome-wide fashion, we compared the circadian liver transcriptomes of mice fed with or without Dox by Affymetrix (MOUSE 430 2.0) microarray hybridization (for details and data analysis, see Materials and Methods, the microarray data are available from the ArrayExpress repository [http://www.ebi.ac.uk/arrayexpress/] under accession number: E-MEXP-842). This analysis revealed 351 circadian transcripts (represented by 432 feature sets) for Dox-treated animals, including most mRNAs known to fluctuate with a robust daily amplitude (e.g., mPer1, mPer2, mPer3, mCry1, Rev-erbα, Rev-erbβ, Bmal1, Clock, Dbp, Tef, Nocturnin, Rorγ, E4bp4, Cyp7a1, or Alas1). In keeping with previous studies [23–27], many cyclically expressed genes are involved in various aspects of liver physiology such as xenobiotic detoxification (e.g., P450 oxidoreductase, Por, Cyp2b9, Cyp2b10, Cyp2g1, and Fmo5), carbohydrate and energy metabolism (e.g., Gk, and Pepck), or lipid and sterol homeostasis (e.g., Elovl3, Insig2, Lipin1, and Cyp7a1). Importantly, the cyclic expression of most rhythmically active genes appeared to depend on an intact hepatocyte oscillator, as the amplitude of circadian accumulation was greatly affected in animals not receiving Dox-supplemented food (Figure 4). Nevertheless, using the algorithms described in Materials and Methods, we identified 31 different transcripts (represented by 41 feature sets), whose circadian accumulation was not affected by the Dox treatment. These are listed in the phase maps of Figure 5A (compare left and right panels), and the circadian expression of some of these genes in the presence and absence of Dox has been validated by Northern blot hybridization (Figure 5B). As expected on the basis of the results displayed in Figure 2, mPer2 mRNA was included among the transcripts whose cyclic accumulation was controlled by systemic cues. Other genes whose transcripts accumulate with phase angles similar to that of mPer2 mRNA were the heat-shock protein genes Hspca (encoding HSP90), Hspa8 (encoding HSP70 isoform 8), Hspa1b (encoding HSP70 isoform 1A), Hsp105 (encoding HSP105), and Stip1 (encoding Stress-Induced Phoshoprotein 1, also known as Hsp70/Hsp90 organizing protein), and a tubulin gene (Tuba4). These genes were expressed in phase with mPer2, suggesting that their cyclic transcription was perhaps governed by similar systemic timing cues. Nocturnin (Ccrn4l), Fus, Chordc1, and Cirbp were additional genes whose circadian expression appeared to be system driven. However, the transcripts issued by these genes belonged to different phase clusters (Figure 5A and 5B), and their synthesis must thus have been regulated by mechanisms different from those governing rhythmic Hsp and/or mPer2 transcription. Particularly interesting was the diurnal expression of heat-shock protein genes and Cirbp, a gene encoding a cold-induced RNA-binding protein. While heat-shock protein mRNAs reached zenith levels at Zeitgeber times when body temperature was maximal, Cirbp mRNA levels peaked at Zeitgeber times when body temperature was minimal [5,32]. Hence temperature cycles oscillating by only a few degrees (35 °C to 38 °C) appeared to be translated into antiphasic Hsp and Cirbp expression cycles.
LAP-tTA/TRE-Rev-erbα mice fed with Dox-supplemented chow (+Dox) or normal chow (−Dox) were sacrificed at twelve 4-h intervals, and the liver transcriptomes were profiled by Affymetrix oligonucleotide microarray hybridization. Circadian transcripts were retrieved as outlined in Materials and Methods from the 24 datasets, and their temporal expression patterns were aligned according to phase. Note that the circadian accumulation of most transcripts is severely blunted in the livers of untreated mice. The heat scale to the right of the panels represents amplitudes in a linear scale, where green and red represent minimal and maximal expression levels, respectively.
(A) A subset of transcripts whose circadian accumulation is not significantly affected by the HA-REV-ERBα overexpression is displayed according to the criteria used for (A). We conclude that the circadian rhythms of these genes are driven by systemic timing cues.
(B) Northern blot quantification of some systemically driven circadian genes. Some of the results of (A) were validated by Northern blotting. For all six genes tested (Hsp105, Hspca, Fus, Nocturnin/Ccrn4l, Cirbp, and mPer2), neither amplitude nor phase is affected in a significant manner by REV-ERBα overexpression. The sharpness of the bands for 18S RNA and Cirbp are due to a shorter migration of this particular formaldehyde agarose gel.
We also considered the possibility that some systemically regulated liver genes could display mRNA accumulation cycles with higher amplitudes in the absence of functional hepatocyte oscillators. For example, the accumulation of liver transcripts whose synthesis is influenced by local oscillators and systemic cues in an antiphasic manner may only be circadian in mice not containing hepatocyte clocks. However, our failure to identify such transcripts did not support such a regulatory mode (see Figure S3A and S3B, and corresponding figure legends), we thus feel that few if any genes produce robust daily mRNA accumulation cycles only in the absence of functional hepatocyte clocks.
Temperature-Dependence of mPer2 Expression
The expression of mPer2 and Hsp appears to be similar with respect to systemic regulation. We thus suspected that a common regulator might influence the transcription of these genes. Since Hsp transcription is governed primarily by heat-shock transcription factors (HSF) [33], we wondered whether mPer2 transcription was also inducible by elevated temperature. In order to examine this conjecture, we incubated cultured organ explants from LAP-tTA/TRE-Rev-erbα/mPer2::luc mice during 150 min at 40 °C (Figure 6) and recorded bioluminescence in real time. Although luciferase activity was somewhat decreased during the heat shock itself, presumably due to a general inhibition of translation [34], a subsequent strong increase in luciferase activity was observed in both liver and lung. Liver explants cultured in the absence of Dox showed a consistent 2-fold enhancement of luciferase activity, suggesting that the heat-dependent regulation of mPer2 did not require a circadian clock (Figure 6A). Lung explants, in which circadian oscillators are operative under these conditions (see Figures 3B and S2B), displayed a phase-specific induction of temperature-induced luciferase activity (Figure 6B). Thus, when the heat shock was performed at a circadian time at which luciferase activity was minimal, a strong induction was observed. On the other hand, a heat shock performed at a circadian time when luciferase activity was maximal did not result in a noteworthy increase in luciferase activity. Taken together, these results indicate that mPer2 is heat inducible and that the strength of this induction is gated by circadian time.
(A) Liver explants of LAP-tTA/TRE-Rev-erbα/mPer2::luc mice were cultured as in Figure 3 and subjected to heat shock (150 min at ∼40 °C) using homemade culture-dish heating devices, and luminescence was recorded as in Figure 3. Temperature plots are extrapolated from periodic temperature measurement. The time window during which organ cultures were exposed to an elevated temperature is depicted by a grey box.
(B) Lung explants were subjected to heat shock as in (A) at two different circadian times.
The minimal HSF binding sites (heat-shock elements [HSEs]) consist of two or more inverted or everted repeats of the pentameric sequence 5′-NGAAN-3′ (where N can be any nucleotide). Taking the two complementary DNA strands into consideration, the statistical frequency of HSEs is approximately 1/2,000 in random DNA, and it is thus impossible to identify functional HSEs solely by sequence inspection. Nevertheless, known functional HSEs are located within 5′-flanking regions of heat-shock protein genes [35], and the sequence analysis of mPer2 revealed a cluster of five HSEs within the 1,700 base pairs (bp) located upstream of the transcription initiation site (Figure S4). Of note, one of these elements (centered around −1,630) lies within a 22-bp sequence block that is 100% identical in mouse, rat, human, and dog (Figure S4). Whether this or any other HSEs displayed in Figure S4 are involved in the temperature-regulation of mPer2 will have to be examined by site-directed mutagenesis and chromatin immunoprecipitation experiments.
We generated a mouse model system in which hepatocyte circadian oscillators can be attenuated in a conditional fashion. The system is based on the tetracycline-dependent, liver-specific overexpression of the nuclear orphan receptor REV-Erbα, a potent repressor of the essential clock gene Bmal1. Thus, when the tetracycline analog doxycycline was omitted from the food, REV-ERBα accumulated to high levels throughout the day and thereby inhibited Bmal1 transcription constitutively in LAP-tTA/TRE-Rev-erbα mice. As a consequence, the expression of obligatory Bmal1 target genes was decreased to a level that no longer supports local oscillator function. When doxycycline was added to the food, the Rev-erbα transgene was silenced, and hepatocyte oscillator function was reestablished in LAP-tTA/TRE-Rev-erbα mice.
By using this novel mouse model, we were able to discriminate between genes whose cyclic expression is driven either by local hepatocyte oscillators or by systemic circadian cues that are controlled directly or indirectly by the SCN. The transcription of liver genes whose expression displayed daily oscillations in LAP-tTA/TRE-Rev-erbα mice despite arrested hepatocyte clocks are likely under the control of physical and/or chemical cues whose systemic rhythms are driven by the central SCN pacemaker. Such systemically regulated genes are expected to include genes involved in the synchronization of hepatocyte clocks. Genome-wide profiling of the liver circadian transcriptome of LAP-tTA/TRE-Rev-erbα mice fed with Dox-supplemented chow revealed about 350 transcripts with robust circadian accumulation. Less than 10% of these transcripts displayed rhythmic accumulation with high amplitude and magnitude in mice fed with normal chow, suggesting that the cyclic transcription of most circadian genes is influenced by local oscillators. We cannot formally exclude that the cyclic expression of some of the genes resilient to HA-REV-ERBα overexpression was driven by a second, yet unknown and BMAL1-independent oscillator. However, this hypothesis clearly did not apply to mPer2, since mPER2::LUC expression ceased to be rhythmic in liver explants not treated with Dox. We thus consider it more likely that the rhythmic expression of genes in the absence of Dox was governed by systemic cues, which were directly or indirectly controlled by the master pacemaker in the SCN. As illustrated in Figure 7, the system-driven circadian expression of mPer2 is of particular interest with regard to the entrainment of the peripheral oscillators by SCN-borne timing cues. The oscillatory mechanism that is at the center of the circadian clock is thought to involve a negative feedback of CRYs and PERs on their own transcription. An externally driven mPer2 transcription cycle would thus gate the phase of the peripheral clock to that of the systemic signals. Indeed, the system-driven expression of mPer2 provides a direct link between circadian systemic signals and the phase of peripheral oscillators. Although the molecular mechanisms responsible for system-driven mPer2 transcription remain to be identified, the observation that many heat-shock protein genes were found to be expressed in phase with mPer2 suggests that the cyclic transcription of mPer2 and Hsp genes shares certain regulatory mechanisms. Of note, real-time bioluminescence recordings of mPer2::luc-expressing liver and lung explants exposed to a heat shock showed that mPer2 transcription can indeed be influenced by temperature. Moreover, the 5′-flanking region of mPer2 harbors five heat-shock response elements (HSEs) within 1,700 bp, of which one is 100% identical in mouse, rat, man, and dog. The identification of the physiologically relevant HSEs within the mPer2 gene will be particularly important, since the activity of HSF1 can also be influenced by chemical cues (e.g., oxidants) [33]. As feeding cycles are the most dominant Zeitgebers for peripheral clocks thus far identified, it is tempting to speculate that HSF1 senses rhythmic metabolism and thereby synchronizes peripheral clocks by gating mPer2 expression.
In the intact animal, the phase of circadian mPer2 cycles is dictated by systemic Zeitgeber cues such as temperature or chemical cues influencing HSF activity (see text). Since mPER2 is also an integral part of the clockwork circuitry, this protein might confer the phase of systemic Zeitgebers to the local oscillator. If the oscillator is inactivated (e.g., by the repression of Bmal1), mPER2 is still expressed in a circadian manner in the intact animal. Under free-running conditions (i.e., in liver explants cultured in vitro), rhythmic mPer2 expression persists, but with the phase and period imposed by the local oscillators. However, when this oscillator is arrested, the expression of mPer2 and probably that of all clock and clock-controlled genes becomes arrhythmic.
Similar to certain nuclear hormone receptors, HSF1 forms functionally inert cytoplasmic complexes with chaperones and co-chaperones in the absence of activating cues [36]. Upon exposure to elevated temperature, oxidative stress, heavy metals, or endobiotic substances (e.g., arachidonic acid), HSF1 gets activated in multiple consecutive steps [37]. These comprise: release from chaperones and co-chaperones, trimerization via an unmasked coiled-coil domain, binding to its cognate DNA sequences in regulatory regions of target genes, and stimulation of the transactivation potential via the calcium/calmodulin-dependent kinase II (CAMKII)-mediated phosphorylation of a serine residue within the HSF1 regulatory domain [38]. Of note, CamkIIb mRNA is among the transcripts whose diurnal accumulation is governed by systemic cues and whose phase is in keeping with a role of CAMKIIb in the circadian activation of HSF1 (see Figure 5A). In addition, CAMKIIb might also participate in the synchronization of peripheral clocks by a more direct mechanism. Thus, in fruit fly cells, CAMKII phosphorylates CLK, the Drosophila ortholog of CLOCK, and in cotransfection experiments. this enhances the stimulation of CLK-CYC target genes [39].
Nocturnin and Cirbp are two systemically driven genes encoding proteins potentially involved in mRNA metabolism and/or activity. Nocturnin, the vertebrate homolog of yeast CCR4, is an mRNA deadenylase [40] with rhythmic expression in many mouse tissues [41]. As both mRNA stability and translation efficiency can depend on poly(A) length, Nocturnin could influence the rhythmic accumulation of circadian proteins by post-transcriptional mechanisms. Likewise, CIRBP, a nuclear, ubiquitously expressed RNA-binding protein [42], could affect the cyclic accumulation or translation of target mRNAs in a temperature-dependent fashion, as diurnal Cirbp expression correlates negatively with body temperature rhythms. In addition, CIRBP has been demonstrated to activate the extracellular signal-regulated kinase (ERK) pathway in NIH3T3 fibroblasts [43]. Similar to CAMKII, Drosophila ERK2 can phosphorylate CLK and thereby increase the transactivation potential of this transcription factor. Cirbp mRNA and protein levels have previously been found to oscillate in brain, but not in liver [32]. However, in the latter tissue, the accumulation of Cirbp transcripts has been determined only for two time points, and the cyclic accumulation of Cirbp mRNA in liver (see Figure 5B) may thus have escaped this analysis. The similarly high amplitude of diurnal Cirbp mRNA accumulation in brain [32] and liver (this study) is somewhat surprising. In fact, most brain areas display much shallower accumulation cycles for clock and clock-controlled mRNAs than the liver. For example, circadian mPer1 and Dbp mRNA levels oscillate about 13-fold and 100-fold, respectively, in liver, but only about 1.4-fold and 2-fold, respectively, in brain [44]. The low-amplitude rhythms in the latter tissue may be the consequence of an incomplete phase entrainment of local oscillators in brain neurons by the SCN, perhaps because the transport of chemical timing cues across the blood-brain barrier is inefficient. The high amplitude of Cirbp expression in the brain may thus be caused by daily temperature fluctuations, which have similar amplitudes in the brain and peripheral organs [45].
In conclusion, we have established a transgenic mouse model that allowed us to study rhythmic liver gene expression genome-wide in the presence and absence of functional hepatocyte oscillators. The identification of genes whose amplitude and phase are nearly identical under these two conditions revealed possible mechanisms by which peripheral oscillators could be entrained. The observation that in liver the circadian expression of mPer2 can be governed by both systemic cues and hepatocyte oscillators provides a plausible mechanism for the phase entrainment of molecular oscillators in peripheral tissues. Strikingly, heat-induced and cold-induced genes were also identified among the genes whose rhythmic expression is driven by systemic cues. Of note, body temperature rhythms have previously been shown to contribute to the phase entrainment of peripheral clocks [8], and it is thus tempting to speculate that the molecular mechanism governing temperature-dependent Hsp and/or Cirbp expression are involved in this process. We feel confident that the in-depth analysis of cis-acting regulatory elements and transcription factors participating in the systemic control of circadian gene transcription will provide valuable information on the phase-entrainment pathways operative in peripheral tissues.
Materials and Methods
Generation of TRE-Rev-erbα transgenic mice.
TRE-Rev-erbα mice were generated by pronuclear injection as described in [46]. A cDNA containing the full-length REV-ERBα coding sequence was obtained from F. Damiola. This cDNA contains the first 134 bp of the mouse cDNA (up to the BamHI site) preceded by two HA tags and followed by the remaining of the rat REV-ERBα sequence (F. Damiola and U. Schibler, unpublished data). The cDNA sequence was then PCR amplified using the primers "Bcl1-HA-Revα" and "Bcl1-downstream-Revα" (see Table S1). The PCR product was cut with BclI and cloned into the BamHI site of the pTRE-2 plasmid (ClonTech, Mountain View, California, United States). This new plasmid was then cleaved with XhoI and AseI, and the resulting 3,645-bp fragment encompassing the seven TREs, the minimal CMV promoter, and the HA-tagged REV-ERBα ORF followed by the rabbit β-globin 3′UTR, was used for microinjection into the pronuclei of mouse zygotes. The transgenic mouse line used in this study was selected among 21 lines obtained from 21 different founder mice for its high and strictly Dox-dependent expression of the TRE-Rev-erbα transgene (as assessed by TaqMan real-time RT-PCR).
Dox treatment.
Dox-containing food pellets were produced as follows: powdered mouse chow (Provimi Kliba, Kaiseraugst, Switzerland) was mixed with an equal weight of water containing 3-g/l Dox (Ufamed, Sursee, Switzerland). The suspension was allowed to stand for a few hours in order to saturate the powder with the Dox solution. Small pellets were then formed, and the water was removed by vacuum lyophilization. Mice were fed with these food pellets for at least 1 wk before they were sacrificed for the analysis of RNA and protein.
Determination of the transgene insertion sites.
We determined the chromosomal insertion site for the TRE-Rev-erbα and LAP-tTA transgenes in order to facilitate the genotyping analysis of transgenic mice by PCR experiments. To this end, transgenic genomic DNA was digested with a frequently cutting restriction enzyme that cleaves the transgene at defined sites (NlaIII for TRE-Rev-erbα and Sau3AI for LAP-tTA). After heat inactivation of the restriction enzyme, the DNA was diluted to a concentration of 2 ng/μl and ligated with T4 DNA ligase in order to circularize the DNA restriction fragments. These DNA fragments were then precipitated and re-linearized with an infrequently cutting restriction enzyme (SacI in both cases) that cuts the transgene between the restriction sites previously used for the production of circularized DNA fragments (composed of transgene and flanking genomic sequences). The DNA was then used for PCR amplification with primers "TRE-fwd" and "TRE-rev" for TRE-Rev-erbα and "pLAP-fwd" and "pLAP-rev" for LAP-tTA (see Table S1). The resulting PCR products were sequenced and the insertion sites determined.
Lap-tTA genotyping was performed by PCR using the primers "LAPtTAtg-fwd" for the transgenic allele, "LAP-tTAwt-fwd2" for the wild-type (WT) allele, and "LAPtTA-rev2" as common reverse primer (see Table S1). The resulting PCR products encompass 302 bp for the WT allele and 360 bp for the transgenic allele. Genotyping of TRE-Rev-erbα was performed by PCR using the primers "twdTRE" for the transgenic allele, 'TREvalphaWT2" for the WT allele, and 'TREvalpha-rev2" as common reverse primer (see Table S1). The resulting PCR products span 351 bp for the WT allele and 560 for the transgenic allele. All experiments shown in the main text of the paper were conducted using double homozygous mice for both Lap-tTA and TRE-Rev-erbα in order to maximize the expression of the transgene, whereas all mice used for in vitro liver explants were heterozygous for the mPER2::LUC allele. The experiments presented in Figure S2 were performed with Lap-tTA/TRE-Rev-erbα/mPER2::LUC triple heterozygous mice for reasons outlined in the legend to this figure.
RNA analysis.
RNA expression levels were determined using whole-cell RNA essentially as described in [16]. Liver whole-cell RNA was extracted according to reference [47], and Northern blot experiments were performed using 5 μg of whole-cell RNA and hybridization to radiolabeled DNA probes according to the Church protocol [48]. Bmal1 and mPer2 specific probes were generated using sequences encompassing the entire open reading frames as templates. For the Northern blot experiments displayed in Figure 5B, hybridization probes were generated from cloned PCR products encompassing the following sequences: bp 1,144 to 1,946 of Nocturnin, bp 429 to 1,583 of Fus, bp 2,325 to 2,991 of Hsp105/110, bp 1,032 to 1,784 of Hspca/Hsp90, and bp 552 to 1,139 of Cirbp. Real-time TaqMan RT-PCR was performed as described [16]. The primers and probes used in this study are all listed in Table S1. A primer-probe set for the Tata binding protein (TBP) transcript was used for normalization. The same TaqMan probe was used for mouse and rat Rev-erbα, because the mouse and rat sequences are identical in the region encompassing this DNA segment.
Western blotting.
Nuclear extracts were prepared by the NUN procedure as described [49], and Western blotting was performed according to standard protocols using affinity-purified rabbit polyclonal antibodies. αCry1, αCry2, αPer1, αPer2, αBmal1, and αREV-ERBα antibodies were kindly provided by S. Brown and J. Ripperger.
Bioluminescence analysis in liver explants.
Bioluminescence measurements of liver slices were performed essentially as described [2]. Mice were sacrificed by decapitation. The inferior vena cava was cut, and ice-cold Hank's Balanced Salt Solution (HBSS, Sigma Cat no. H1641; St. Louis, Missouri, United States) was perfused through the spleen in order to remove blood and refrigerate the liver. Tissue pieces were removed, placed into ice-cold HBSS, and sliced into smaller fragments (volume approximately 10 mm3). These tissue pieces were then placed on glass fiber filters in 35-mm tissue culture dishes containing 1.2–1.5 ml of HEPES-buffered phenol red-free DMEM (GIBCO Cat no. 1741; San Diego, California, United States) supplemented with 5% fetal calf serum, 2 mM glutamine, 100-U/ml penicillin,100-μg/ml streptomycin, and 0.1 mM luciferin. Only distal edges of the liver lobes were used, since they gave more reproducible and persistent cycles, perhaps owing to their favorable surface/volume ratio. When relevant, Dox was added to a final concentration of 10 μg/ml. Cultures were maintained at 37 °C in a light-tight incubator, and bioluminescence was monitored continuously using Hamamatsu photomultiplier tubes (PMT; Hamamatsu, Hamamatsu City, Japan) [31]. Photon counts were integrated over 1-min intervals. Dox pretreatment of animals (Figure 3) consisted of two intraperitoneal injections of 2-mg Dox in PBS. Temperature variations in tissue explant cultures were generated using homemade programmable heating chambers.
Microarray hybridization.
Thirty-six male mice double homozygous for the two transgenes fed with normal chow and an equal number of mice fed with Dox-supplemented chow were maintained on an LD12:12 light cycle and were used for these experiments. Six animals each for Dox-treated and untreated mice were sacrificed at ZT00, ZT04, ZT08, ZT12, ZT16, and ZT20, and whole-cell liver RNA was extracted from each animal. RNA pools of three male animals were assembled by mixing equal amounts of RNA. This resulted in 12 temporally staged RNA pools representing two entire days. A total of 5 μg of pooled RNA was used for the synthesis of biotinylated cRNA; 8.75 μg of biotinylated cRNA was then hybridized to 24 mouse Affymetrix 430 2.0 chips containing 45,000 feature sets and representing 39,000 genes, using standard Affymetrix protocols. The chips were washed and scanned, and the fluorescence signals were analyzed with the RMAexpress software using Robust Multi-array Analysis (RMA) [50].
The data thus obtained were used for a Fourier transform analysis, and the ratio of the F24 spectral power to the sum of the other Fourier components (i.e., ∞, 48, 12, 9.6, and 8 h) was calculated for each feature set [51]. The time points were then permuted 50,000 times, and for each of the permutations, the Fourier transform was calculated together with the ratio of the F24 Fourier component to the other components [52]. Expression data were fitted to a cosine curve. Temporal mRNA accumulation was considered as circadian if its amplitude was higher than 2-fold and if the ratio of the unscrambled Fourier transform was within the top 5% of the scrambled ratios. In order to find circadian genes unaffected by Dox treatment, we calculated a divergence (D) coefficient as follows: where ZT0:ZT44 represents the hybridization signal for the specified time points. We did not apply any filter for "presence" or "absence" flags (as determined by the MAS Affymetrix software), since 95% of the hybridization signals obtained for the 432 feature sets that we qualified as circadian by Fourier transform analysis scored as "present" in more than 12 experiments out of 24 by the MAS software. By comparison, only 35% of total (circadian and non-circadian) features sets were considered as present in at least 12 experiments.
Supporting Information
Figure S1. Quantification of Bmal1 Protein Levels in TRE-Rev-erbα/LAP-tTA Transgenic Mice
Figure S2. Temporal Luminescence Profiles of Liver and Lung Explants from Triple Heterozygous TRE-Rev-erbα/LAP-tTA/mPer2::luc Triple Transgenic Mice
Figure S3. Detection of Transcripts Displaying Circadian Accumulation Profiles in Mice Fed with Normal Chow (−Dox)
Figure S4. Identification of Potential HSF Binding Sites in the mPer2 Gene
Table S1. PCR Primers and Probes Used in This Study
Accession Numbers
The GenBank (http://www.ncbi.nlm.nih.gov/Genbank) accession numbers for the genes and gene products discussed in this paper are Cirbp (NM_007705), Fus (NM_139149), Hsp105/110 (NM_013559), Hspca/Hsp90 (NM_010480), and Nocturnin (NM_009834).
The ArrayExpress repository (http://www.ebi.ac.uk/arrayexpress) accession number for the microarray data is E-MEXP-842.
We are grateful to Joszef Zakani for his advice on the generation of transgenic mice, to Patrick Descombes, Didier Chollet, and Michel Kocher for their valuable help in Affymetrix transcriptome profiling, to Nicolas Roggli for his expert preparation of illustrations, to André Liani for designing and constructing programmable heating/cooling chambers, and to Charna Dibner, Hans Reinke, David Gatfield, and Markus Stratmann for their critical comments on the manuscript.
Author Contributions
BK, HB, and US conceived and designed the experiments. BK performed the experiments. BK, OS, and US analyzed the data. HB and JST contributed reagents/materials/analysis tools. BK and US wrote the paper.
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text: Tape a Picture of a Face to Your Webcam to Become More Comfortable on VideoMelanie Pinola3/12/13 9:30amFiled to: VideoWebcamsMind HacksClever Uses21EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalink Do you tend to freeze up or get nervous when you're speaking to the webcam? One simple trick that might help you become more comfortable on screen is to use a picture of a person (drawn or a photograph) and place it in front of the camera—with a cutout for the camera eye, of course. That's it. As blogger Randy Wilson explains in his video above, instead of the strangeness of talking to a camera eye when you're in a video chat or recording yourself, you'll have a face to talk to. It'll also help you focus on looking into the camera directly when you speak. How to Be More Comfortable on Camera | Learn Success The Easy WayReply21 repliesLeave a reply

subset: commoncrawl


text:
Adriana Karembeu and Aram Ohanian (businessman) have been married since Jun 2014. [view couple]
Adriana Sklenaříková born on 17 September 1971 is a Slovak fashion model and actress. She is a former Guinness record holder for the longest legs among female models (at almost 1.24 m)....
Connect any celebrity with Adriana Karembeu to see how closely they are linked... romantically!
She is so pretty i love h e r !
mmm nice looking girl
hello carembeu dwiche frpm casablanca
she had an argument with ehr bf and went on a mn hunting spree.in one night she was found in intimate scenarios with a taxi driver,including a pool threesome and a spa foursome


text: We Still Need an Electrician to Help Out at Gizmodo Gallery!!!Adrian Covert9/19/09 11:30amFiled to: Giz Gallery '09Gizmodo Gallery20EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalinkWe've started setting up Gizmodo Gallery and we still need someone with electrical expertise to come in and wire us up a couple of outlets that run on a 220v circuit and string some extension cords for us. And we're willing to pay you MONEY. If yourself, or someone you know is up to the task, shoot an email over to email@example.com and we can talk. We'd love to get someone in ASAP so that we can bring the 103-inch TV and automatic pancake maker to the masses!!!Reply20 repliesLeave a reply


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Upcoming Photography, Photoshop and Lightroom Events
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Location: Berkeley Heights, NJ (Location will be provided to those who register) Fee: $169 for Workshop Fee includes a printed course guide, calibration print for screen... Learn more
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This Meetup is a little different than normal. I'm announcing another group's Meetup in Milltown, NJ. This one is a free photo lecture by Peter Alessandria, and it will... Learn more
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text:
Ill probably just wait till that under $15 sale which may likely be sometime next year. Ive waited one year whats another? Not like youll run out of games to play!
That if you create a female character that shows a lil ass that there will be a big backlash in the gaming community, and as such will create a lot of buzz and traffic to your game!
Ass= MMO success! Maybe thats why battleborn didnt do as(s) well!
This trailer wasnt very good. It felt like it was trying to tell me that "being a hacker is fun and hip and with it and so we should stick it to the man!" Its seemed like these teens maybe were creating chaos just cuz they could for no real reason.
That gamers love some good ole blasts from the past from time to time.
Is it only on Xbox? God I loved playing this game on my PC! Would love to play again!
completely agree
Whoa! Thats a bit too beautiful and a bit too much water! IGNs score for this game gonna be hella low now.
No farming/grinding of Titanites needed? We'll see. ( ͡° ͜ʖ ͡°)
The ending. The eeeennnnddddinnnngggggg.
Still up for debate if I will get it or not.
Cuz Im wondering if there will be a story like Destinys where you complete the story (Year 1) and should then focus on PVP? Divisions PVP being Dark Zone.
Beta impressions:
Majority of Buildings will be closed so youll have to be running on the streets and going around the block instead going inside a structure to use as a shortcut.
Dont know if receiving money wi...
Yeah but that Dark Cloud 2 is really what I care most about in this weeks PSN update!
Should there be censorship in video games?
Yes. And while your at it please censor films and television where youll have more people seeing what shouldnt be seen!
Like most my hype for this game has severly fallen and this trailer has risen it back slightly but i will actually wait for once for what reviewers say.
But my biggest worry is Ubis servers I have Rainbow six siege right now and there is severe lag, glitches like falling through the map, body parts sticking out of the walls for easy kills, application forced quit, and long matchmaking waiting times.
Ive got most these except for FO3 and Minecraft, but I do have Fallout 4s which is funny
The whole article reads as PR talk doesnt seem genuine in the IMO.
No lie a YEAR later? I will most likely forget its existence until I see an article that says its now $30 or whatnot. Seeing as their will be a plethora of games coming out next year!
It doesnt surprise me one bit! Xbox One was still more expensive ($349) than the PS4 ($300) on Black Friday!
This feels like it was inspired by Resogun with all those materials flying around when enemies are destroyed! Interesting must wait for more!
Minor Backlash? Lol with many forum threads and reddits being made with people saying they wont be playing Destiny cuz of this DLC? Sure. But at least they actually did something bout it!


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LOS ANGELES — On any other night, Kings defenseman Jack Johnson likely would have been the player lit up by cameras and surrounded by tape recorders.
But a few stalls to his right was goalie Jonathan Quick, basking in the glow of his franchise-record third consecutive shutout in a 1-0 victory Saturday night against the Dallas Stars at Staples Center.
The milestone might not have been possible if not for Johnson's blast from just inside the Dallas blue line following a face-off win by teammate Mike Richards with about 15 minutes remaining in the third period. The puck made its way out of the circle to Johnson's defensive partner, Rob Scuderi, who slid it to Johnson on his right.
With multiple bodies between Johnson and Dallas back-up goalie Andrew Raycroft, the puck knuckled through and hit the net just inside the far post for his third game-winner of the season. Johnson, in his sixth year in the NHL, had never had a game winner before this season.
"We're all really happy to win it for [Quick]," Johnson said. "He has been playing great for us all year, and he's been playing great ever since he got here, so he deserves this as much as anyone. Hopefully, he doesn't stop at three."
For most of the night, Dallas had made it tough on the Kings to get shots through. They blocked 20 shots through two periods and finished with 26.
"They were blocking a ton of shots," Johnson said. "I was just trying to get it by the first guy, make it high and hard enough so that if they were going to block it, hopefully, it would hurt one of them. Make them think twice about it."
Kings coach Terry Murray said he couldn't remember the last time the Kings had scored off a face off. He said the play was worked to perfection, however.
"It's one of those plays, we talk about it often, where you just want to shoot the puck and get it to the net," he said. "You've got their five players and you've got our three forwards down there in that mix off the face-off win, and that's why it's critical to shoot the puck off a clean face off."
Johnson's other two game-winners this season came during 4-on-3 power plays in overtime.
"He's on fire," said Kings center Anze Kopitar. "Every time the game's on the line he steps up. Tonight, he fired it from the blue line and the puck had eyes and went in."
And the rest was history.


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China makes way, way too much steel. In 2015, it produced nearly 500 million tons more than it needed. Production of that steel was subsidized by the Chinese government in ways that violate international trade rules, so the price was artificially low. And China suppresses the value of its currency, further falsely reducing the cost of the steel.
The fair trade movement and concern about ethical sourcing asks consumers, all of us, to think more carefully about what we buy, and to ask hard questions about how producers behave. Air travelers who believe they are people of good will, people who embrace the Golden Rule, should ask the same question when booking a trip.
I live in one of the most romantic towns in the world, San Miguel de Allende, and I had not had a single date since I arrived 15 years ago. I arrived depressed, hoping to disappear into the life of an expat in Mexico, after closing my beloved independent bookstore in Berkeley and filing for divorce.
The systemically low prices in cocoa have drastic consequences for farmers and their families. More than 2 million children in the Ivory Coast and Ghana are being deprived of their childhoods, either working in extremely hazardous conditions or working in lieu of going to school, so that we can get our chocolate fix.


text:
PS2 Friday, March 31, 2006 | 11:51 AM
.hack//GU Part 1 trailer
Japan March trailer
Download: AVI
All comments
anyway I don't care, going to be great..
I don't know if you would like the game if you didn't like the first 4..but hey, it should still be a great RPG. Glad to see the playstation 2 still has life! and great JRPGS!
And the trailer looks awesome. The battle system in the previous hacks wasn't that good (it was passable, but hardly even that), but I absolutely loved the story. This seems to fix the bad battle system, and the plot seems interesting. Definitely one of my Most Wanted games at the moment (after FFXII and Okami).
About the game
- .hack//GU Part 1: RESURRECTION
- Published by Bandai co
- French release: 3rd quarter 2006
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- Red Dead Redemption on Xbox One Backward Compatibility
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- Koei Tecmo announces Berserk (working title)
- Techland officially becomes a global video game publisher
- No Man's Sky delayed to August 9
- Mass Effect: Andromeda will now ship in early 2017
- EA & Respawn developing a new Star Wars game
- Dishonored 2 will release worldwide on November 11
- Alicia Vikander to star as Lara Croft in Tomb Raider
- Patrice Désilets and Ubisoft settle on 1666 Amsterdam
- Lionsgate launches over 100 films on Steam
- Mirror's Edge: Catalyst release date pushed to June 7
- Dark Souls The Board Game on Kickstarter, funded in 3 min.


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Q & A Library
Safe, Sustainable Farmed Salmon?
What can you tell me about the new sustainable farmed salmon? Is it really a safe alternative to ordinary farmed salmon?
Answer (Published 3/4/2010)
You're referring to a specific method for farming Pacific coho salmon that was approved in January 2010 by the Monterey Bay Aquarium's Seafood WATCH program. (Seafood WATCH is the aquarium's effort to raise awareness of the link between the seafood we eat and the health of the oceans.)
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A little background on farmed salmon: almost all salmon served today is farmed ("Atlantic salmon"), which has less flavor, less protein, and more fat than wild salmon, and its health promoting ratio of omega-6 to omega-3 fatty acids may not be as favorable as in wild fish. It likely also contains residues of pesticides, antibiotics and other drugs used to control diseases that occur when fish are crowded together in the open-net pens of fish farms. Those diseases may escape and decimate wild populations of salmon. Moreover, it takes several pounds of feed fish to produce one pound of salmon. The net result is greatly accelerated depletion of the numbers of fish in the oceans. That is why I have recommended choosing wild Alaskan salmon. It tastes better, is more nutritious than farmed Atlantic salmon, and eating it does not have the environmental impact or health consequences of relying on farmed salmon.
The news that Seafood WATCH has approved the new salmon farming technique is certainly welcome. I understand that this action followed several months of investigation by scientists who inspected the production facilities and reviewed feed ratios, fish contaminant and pollution discharge levels at the salmon farms. Seafood WATCH explained that the new farming methods contain the fish and water in closed systems, such as tanks, rather than releasing them into the environment. In addition, the use of disease-free source water and strict security protocols "minimize the spread of disease to the wild" and "impact the environment less than open net pens." Its approval of the farming method means that the salmon from these new farms will be assigned a green "best choice" rating on the Seafood WATCH website and pocket guide to ocean-friendly seafood.
In order to be accorded the "best choice" designation, the salmon itself must provide the daily minimum of omega-3 fatty acids (at least 250 milligrams per day) based on 28 grams of fish and have levels of polychlorinated biphenyl (PCB) levels under 11 parts per billion. Limited supplies of the new farmed salmon are scheduled to be introduced in the Pacific Northwest this year. Although my personal favorite is still wild Alaskan salmon, I checked to see how the new tank farmed salmon will be priced, and found that this has not yet been determined.
Andrew Weil, M.D.
More information about Seafood WATCH.
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what kind of price you talking about if its stock? I have a 13 tdi sel and love it wouldnt mind seeing the wife get one as well.
Type: Posts; User: RedWabbitVR
what kind of price you talking about if its stock? I have a 13 tdi sel and love it wouldnt mind seeing the wife get one as well.
It'd be funnier if it wasnt true. :/ the vw tech sent me a video of the car up on the lift he spun the tires and sure enough, pisses me off that I just bought it from the dealer like that.like you...
see now, I saw something similar on a silver passat and it didnt look quite right. but on a black passat those Mirabeaus look nice.
So I bought my passat 12-31-15. Had to take it into the...
Last I recall those werent the headlights you started out with and they dont look like the china ones, where you get those?
also really dissapointed that most of the cars in here are silver,...
i see you have a gas pedal but do you happen to have the clutch pedal?
havent been on in a while but I want to thank you for your help. that was indeed the part and it was really easy to swap out. thanks.
But seriously my cousin brought over her a6 with the 3.0 v6 and I just replaced the stupid intake links that always break. after replacing them I started the engine to make sure they were working...
you should at least give us a clue to what is in the yard sale before making people drive out.
never gave a price for the car
hopefully tomorrow Ill be picking up this car I went and looked at it and did a scan, unfortunately the scan is on my friends laptop so I cant get it atm. But the car could not communicate with the...
couple a questions about this.
1. if one of the injectors were going bad could it build resistance and blow the fuse?
2. if the ground was loose could this also cause and increase resistance...
well I scanned it again today and cleared the codes, right after I did it fired up. I drove it some more tonight and after about 30 minutes in the snow/wet it did it again though instead of shutting...
ah yeah didnt think about that, I had only checked the ones under the hood. It was blown and I replaced but still not wanting to run anything else I should look for? I was thinking of clearing the...
VCDS -- Windows Based VAG/VAS Emulator
VCDS Version: Release 12.12.0 (x64)
Data version: 20130910
VIN: License Plate: WHITEJETTA
You bought a remaned part, they took an old part and fixed what was wrong with it and sold it to you. Ive known quite a few to break shortly after getting them, take it back and get a replacement.
I was interested but not after that smartass remark. If you are gonna be an ass about people asking questions don't post up for a friend.
Sent from under my car.
it was looking good when I drove by at lunch. curious though what where you working on?
:screwy: this looks more like it came out of a mk4? are you sure its a mk3 golf that it came out of?
But the module is saying that it's opening when I pull the button in the door. I even hear a relay clicking when I do it. I'll put a meter to it today when I get home
Sent from under my car.
oh yeah thats the only way to open the trunk. you have to put the key in the trunk to open it it. the remote or the door switch do not work.


text:
Wong Kar-wai's "The Grandmaster" to be screened at Berlinale
February 4, 2013 - 19:01 AMT
PanARMENIAN.Net - Wong Kar-wai has just finished the international version of The Grandmaster, with the auteur trimming 15 more minutes off the cut released in China last month, The Hollywood Reporter said.
The latest version, which will be shown to a selected group of European journalists in Paris before making its bow as the curtain-raiser of the Berlin Film Festival on Feb. 7, represents a further condensation of a film of which the first cut ran to four hours.
Wong eventually edited the film to 130 minutes for its Jan. 8 release in China, where it has since generated healthy box office earnings of US$45 million. The director has hinted in interviews last month that the longer version will be released later as a director's cut.
Revolving around a fictionalized account of the early years of real-life Wing Chun martial arts expert (and Bruce Lee's mentor) Ip Man (played by Tony Leung Chiu-wai), The Grandmaster is shown in Berlin as an out-of-competition entry, with Wong serving as the chairman of the festival jury this year.
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Regency Hotel Miami
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Shuttle bus service
- Suites
- Wireless internet connection in public areas
- Restaurant
- Refrigerator in room
- High-speed internet in room
Photos of Regency Hotel Miami
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- More recommendations
Historical Traveler Reviews Regency Hotel Miami
Decent room, Bad shuttle
I stayed for one night at this hotel because a flight was late. American Airlines comped the stay, but I was in a very bad mood and ready to rest from travelling all day.
Their shuttle service is terrible. It only runs once every 30 minutes. Standing outside the airport I saw several other hotel shuttles go by several times before we saw the small white van that is the hotel's shuttle. There were already two people in the shuttle, and a couple with a small child got on the same time I and my husband did. Then the shuttle stopped again and picked up some flight attendents. There were a total of 15 people in the van: two people were sharing the front seat and a woman sat on someone's lap in the back seat next to us. It was very dangerous and unsafe.
Getting off the shuttle at the hotel, the driver gave the luggage to all the flight attendents first, even though he had a stroller in his hand for the couple with the child. They had to wait to get their stroller. I thought this was very rude.
Aside from that bad experience, the food at the hotel's restraunt was decent. The room was also decent and they were able to give us a king sized bed which was nice. The TV stations such as ABC, NBC, CBS did not come in very well on the TV as there was much static.
I would not recommend someone to stay at this hotel if they want more than a place to sleep for the night.
It is ok
I don't understand what the others tripadvisors expect for a hotel so near the airport and for a reasonable price.
We took 2 rooms and both are clean and comfortable. That�s all what we need for sleep one night in Miami.
We didn't have any problem with the transport, we arrived to late in the night: at 1:00 a.m., called to the hotel and the shuttle came for us.
If you need more than that, like a big pool, spend more time in the hotel or something, maybe you should have to go any where else and pay more.
Not good, even when BA have paid for you......
Well we got sent here by BA having been bumped off our flight...so we weren't in the best of moods.
They are in the middle of doing some refurbishing which maybe didn't help.
Having arrived around 9.30pm what food was left in the restaurant (buffet) was pretty unappetising...
This was especially annoying as BA had given us $25/head allowance....!! Told at check in that most people only get about $7 and reckon you would have struggled to spend that!
Rooms are very antiquated and looking tired, but a reasonable size.
Noise from planes is present but not too bad - no surprise really.
Breakfast was ok.
Good point ? Staff were very good. As mentioned we were not happy bunnies and they certainly did all they could to help.
So-So Hotel, Not Worth the Price
We arrived on Saturday at the Airport Regency after 3pm, but the room was not ready, so the front desk person said we had to wait a half hour. If the hotel states that check-in time is at 3pm, then why should I have to wait? The key cards did not work when trying to ride the elevator, and we had to get replacements. I was told that the cardstrips were worn.
The room was clean and cozy. It was a quiet floor. However, the seafood restaurant next door had live music that night starting at around 11pm and continued until 4am. Obviously it kept me awake. This is downtown Miami, so I suppose the hotel could do nothing about this.
I won't stay there again.
It is as bad as everyone says
This hotel was awful, even for $80/night and a free shuttle to the port. They overbooked the shuttle, so we had to take a taxi. Our 7 year old even commented on how dark and dingy the room was. The staff is discourteous. We canceled our return reservation and stayed at the Embassy Suites. Thank goodness we did. Noone should stay here.
Get what you pay for--Really OK hotel
After reading all of the negative reviews...I had to post . The shuttle service was great. My room was SUPER clean. Front desk people were extremely helpful. Nice restaurants in the area. About 15 minutes away from South Beach, if you rent a car...you'll be all set! This hotel is really a bargain! As a matter of fact...I'm staying @ the Regency this May 2005!
Not nice at all
I ended up staying one night at the Airport Regency because of a flight problem... American Airlines gave us a discount voucher for this hotel as we were forced to spend a night in Miami.
Really, really disorganized check-in. None of the smart keys were working, for some unknown reason, so everyone who checked in had to come back down and ask for housekeeping. The room was unattractive, dirty and smelled of some dreadful flowery cleaner. The blanket was an ugly, scratchy, and very old - it was actually uncomfortable, even with a sheet in betwee. I would NOT recommend this place and feel that even the supposedly low $69/night we paid was far outside of the hotel's value.
Airpory Regency
When we arrived, we checked in, got our room, then went to our room. We were given someone elses room. We walked into someone elses room. Then we went down to the front desk to get a new room. We then enter our new room, the outlets don't work, the carpets are stained, the beds sheets are stained with ink. The bathroom has pubic hair on the floor. Later on we tried watching tv. The tv had no reception. We called orbitz, and called to complain, because the it was just horrible. The called the hotel, the hotel gave us a new room. they gave us the top floor which was is the penthouse floor. The tv worked better, the room was alot cleaner. The hotel is also in the middle of construction, mabey one day is nice. You look at the reception area in the pictures and you say its not that bad. But when you come into most room, it looked bad. the pool is not heated like it stated.
Go somewhere else if you want a clean room
Unfortunately, it wasn't our choice to stay here. Due to American Airlines' (American Eagle) supreme incompetence, we were bumped from our flight and stuck in Miami overnight (another long story I don't wish to relive).
In any case, you would think with the beautiful new marble tile that they were installing in the lobby, that the rest of the hotel was going to be just as wonderful. Not so. The first room we were sent to (rm 512) looked as if the carpets hadn't been cleaned in at least 5 years...maybe 10. No, I am not exaggerating (I have photos if anyone is interested). In addition, the a/c sounded like your own personal airplane in the room, there were holes in the walls where I'm assuming pictures had once hung and some sort of dirt in between the tiles on the ceiling. Wallpaper was starting to roll at the edges and there were some stains on some of the walls. There was a new armoire and headboard, but heaven forbid they clean floors. Someone elses hairs were in the bathroom, and the outdoor balcony had a nice blanket of mold growing in the corner below the a/c unit. The elevator refused to come to the fifth floor unless you got on at a different floor and selected "5" from inside the elevator. The morning we left, we stood for 15 minutes before a service guy happened to show up on our floor and took us down the service elevator. Our second room was only marginally bettert...either that or the fact that the carpet in this room was navy blue, rather than mauve, hid the amount of filth on the floor...in either case, I never walked barefoot...but I recall my sock stepping on a sticky portion of the floor.
I will say that the gentleman manning the desk in the evening (Oscar) was very friendly and helpful...he did his best considering his coworker had injured herself just shortly before our gang of American Airlines bumpees had arrived and she was at the hospital getting helped while he was stuck all alone trying to answer the phone, get us all rooms, and deal with an excruciatingly slow computer operating system. I did not try the restaurant, but was told by a fellow flight bumpee that they only had like 4 items on the menu.
I can also say the shuttle driver was friendly. Basically you have a lot of friendly people working in a poorly maintained hotel. Maybe the fact that they're renovating the lobby means that things will improve eventually, but I don't hold out much hope when someone thinks that renovating the lobby is more important than renovating the rooms that people need to sleep in. Someone needs to get their priorities straight.
My recommendation, give them a few years to get their act together...assuming you're not like us and actually have a choice.
I'll try to add photos here in the next couple days.
Would not do it again....
Bus Driver from Airport was friendly and give us tips on the City the Port of Miami..The Young Lady at the front desk was very nice and helpful .The Restaurant, that was open late, had no food...very limited selection..no hamburgers, no french fries, no salad, no ice for the ice tea???...THEN in the middle of the night all HELL broke loose, guests came in on our floor at 12:00 midnight being very loud, quited down in about a hour ONLY to start up again about 2:30 AM and go on until about 4:00..yelling in the halls, banging, throwing things (or people) against the walls and the phone was out of order???couldn't get the front deesk, couldn't get an outside line, nothing.. Finally about 4:30AM things quited down, phone still didn't work...We did report the incident to the Managers desk in the morning..Got a Sorry about that..and the phones go out often (I thought this was America 2004, and Downtown Miami at that).. OH WELL, WON'T DO THAT AGAIN..There has to be a Hotel in Miami that has Food in the Late Restaurant..Phones that work all night and Guest who arrive late will have some respect for those guests already in their rooms, that might be sleeping at 12:30 to 4:30 AM..that just might be a no brainer..
TripAdvisor Reviews Regency Hotel Miami
Travel Blogs from Miami
Cocktails, Ferraris, bikinis, skin and show
... take a photo of their details. Umm...you are a cab driver,click! And so began our fun and entertainment in Miami.
As our cab turned onto Ocean Drive in search of the Pelican Hotel, our home for the next 2 nights, we were hit with visual over load - traffic resembling a car park that had become a rave for the partially dressed and partially sober and then there were their passengers. Ahh Miami - we were excited already. We pulled up in front ...
... ride! His biggest concern was making sure he didn't lose his flip flops during the ride.
The weather was very hot and sunny but seemed to break into torrential rain and thunderstorms at about 4pm. I was not too bothered about getting wet as ...
Hi there
Hello! How do you feel? I am Anya. I like your page and I want to keep company with you. I suppose, that we can find a common language I intend to write you a letter and I hope to get an answer. I read your profile and I like that you wrote about yourself. Do you have some social networks? I will be waiting for your letter. My Email - firstname.lastname@example.com Best ...
... storm was rolling in over the ocean which gave the water that eerie mirror grey colour with just a few random rays of sunshine coming through. It was time to go, bags were packed and down the elevator we went. We said our goodbyes and before I knew it we in a cab in the pouring rain on our way to the airport. Danielle passed out in the cab, full drop jaw style. Typical. Our flight was delayed a few hours due to the weather which was fine as we decided to buy some booze and ...
Vacation's Over
For our last day in Florida, yesterday, we decided to hang out at the condo. We packed and lounged. Nothing very exciting happened.
Dinner found us at a nearby restaurant, Chuy's Mexican Food. I started with a Pacifico beer. To start, our waitress served us some chips and salsa. The chips were warm and paper thin. They reminded me of the chips we used ...


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