text:
SAN DIEGO (AP) - Jesse Hahn pitched well into the seventh inning against his former team and Stephen Vogt hit a grand slam as the Oakland Athletics beat the San Diego Padres 9-1 on Monday night.
VANCOUVER, British Columbia (AP) - Abby Wambach will do whatever it takes to win the Women's World Cup, even if that means she's coming off the bench.
SAN DIEGO (AP) - Bud Black understood full well the expectations for San Diego to win now with a remade, star-studded roster - and with a new front office, too.
HAYWARD (AP) - Andre Ward is never one to shy away from a fight.
DENVER (AP) - The Denver Nuggets took 105 days to find their new head coach. Now that they've agreed to a deal with Michael Malone, they're off and running.
UNIVERSITY PLACE, Wash. (AP) - Robert Streb walked off the third green at Chambers Bay, looked both directions and headed left down a dirt path toward Puget Sound.
After dealing with anxiety issues, former top-10 player Mardy Fish is going to give professional tennis another try. And this time, he's bringing pal Andy Roddick along with him.
SAN FRANCISCO (AP) - No no-no this time for Chris Heston.
OAKLAND (AP) - Stephen Curry came off a screen, dribbled behind his back and crossed over Matthew Dellavedova. He stepped back and swished a 3-pointer, then pounded his chest and pointed to the roof, seemingly controlling the sellout crowd of 19,596 on his fingertips.
ANAHEIM (AP) - Only twice have the Oakland Athletics had a full-time starting pitcher lead the American League in earned run average at the end of a season. Their names were Catfish Hunter in 1974 and Vida Blue in 1971.
BROOKLYN, Mich. (AP) - Kyle Larson knew the rain was coming - and wanted it to arrive as soon as possible.
CHICAGO (AP) - Kris Versteeg could have sulked or pouted when he was pulled out of the lineup for the Chicago Blackhawks. Instead, the veteran forward proved he belonged on the ice.
WINNIPEG, Manitoba (AP) - Once one of the prominent faces of the U.S. women's team, promoted alongside stars like Alex Morgan and Abby Wambach, Hope Solo is now largely relegated to the background while she faces continued scrutiny over a domestic assault case last year.
HARRISON, N.Y. (AP) - Inbee Park won her third consecutive Women's PGA Championship on Sunday - and accomplished a whole lot more.
Page 164 of 790
NEW YORK (AP) - Technology industry leaders have urged NBA Commissioner Adam Silver to move the All-Star Game out of Charlotte unless a North Carolina law ...
Tim Duncan never wanted the spotlight, only the trophies. He never wanted the endorsements, only the camaraderie. He never wanted the accolades, only the collective ...
First Kobe Bryant, now Tim Duncan. A two-decade chapter of NBA history has closed.
TROON, Scotland (AP) - Golf waited 112 years to get back into the Olympics. The top four players in the world are waiting a bit longer.
SAN DIEGO (AP) - London is not calling for Major League Baseball next year.
TROON, Scotland (AP) - Jason Day is more motivated by failure than success, which helps explain how he reached No. 1 in the world.
PARIS (AP) - The Portuguese party had just begun when a message briefly flickered on the Stade de France big screen: "Merci pour tout, Michel."
LOS ANGELES (AP) - In less than 16 years, the UFC has grown from a money-losing company in a widely reviled sport into a global entertainment ...
SAN DIEGO (AP) - Goose Gossage keeps a bottle of Weibel Vineyards champagne with "Cubs" on the label perched on a shelf in his pantry, a ...
SAN DIEGO (AP) - Chris Sale can admit it now: He was a little too in love with the strikeout.
SAN DIEGO (AP) - Giancarlo Stanton wore out Petco Park with a record display of power in the All-Star Home Run Derby on Monday night, peppering ...
EAST LANSING, Mich. (AP) - Golden State Warriors star Draymond Green was arrested on a charge of misdemeanor assault and battery over the weekend near the ...
NEWTON, Iowa (AP) - Josef Newgarden had made a habit out of finishing second at Iowa Speedway.
EUGENE, Ore. (AP) - Allyson Felix had the star power to change the Olympic schedule.
SAN MARTIN (AP) - Just when it seemed as if the U.S. Women's Open would come down to the final hole of the three-hole ...
Page 11 of 36
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- The Recovery Shop totem...Wednesday 4 May 2016
- A gallery tour with Ron Radford, outgoing Director of the National Gallery of Australia. ...Thursday 18 September 2014
- Dark Mofo festival organisers continue to entice audiences to embrace the dark of winter with a feast of underground artforms in underutilised spaces across the southern capital....Friday 13 June 2014
- Local Hobart artists Sally Rees and Mish Meijers are busy setting up for their exhibition at Contemporary Art Tasmania, formerly known as Cast gallery in North Hobart as they take time out to explain what being an artist means to them. ...Wednesday 16 April 2014
- In the lead up to the 2014 Tasmanian state election there's been a lot of talk and discussion about health, education and jobs within the state, but according to arts consultant Kylie Eastley not enough focus on the creative industries. ...Thursday 13 March 2014
Explore by Topic
Latest Sculpture stories on ABC Hobart
Latest Sculpture stories on ABC Online
- Thursday 12 May 2016
- A Japanese artist arrested in 2014 has been found guilty of breaking the country's obscenity laws after she sent data which enabled people to 3D print a cast of her vagina. Megumi Igarashi produced a series of artworks modelled on her vagina, which were deemed obscene under the Japanese criminal ...Tuesday 10 May 2016
- Members of an Aboriginal community affected by the nuclear bomb tests at Maralinga in the 1950s are connecting with nuclear bomb survivors in Japan. A group of artists from Yalata in South Australia are flying to Nagasaki today to unveil a sculpture for its peace park - it will be the first ...Thursday 14 April 2016
- We look at the life of the Chinese artist Ai Wei Wei....Wednesday 30 March 2016
- Have we warmed to the sculpture dubbed 'The Yellow Peril' when it first took pride of place in Melbourne's city square? Artist Ron Robertson-Swann reveals the echoes of Vault hidden around the city....Friday 19 February 2016

subset: commoncrawl


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New probiotic combats inflammatory bowel disease
You know the probiotics in your peach yogurt are healthful, but now it appears they may also be a powerful treatment for disease.
You know the probiotics in your peach yogurt are healthful, but now it appears they may also be a powerful treatment for disease.
(PhysOrg.com) -- Five decades after the discovery of a rare but potentially pivotal immune cell, scientists at Washington University School of Medicine in St. Louis have found a way to eliminate it in mice.
Scientists from the Walter and Eliza Hall Institute have identified the key immune cell population responsible for regulating the body's immune response.
Scientists have identified genetic errors in women with autoimmune diseases that increase the risk of preeclampsia, a potentially life-threatening condition that occurs in 10 percent of all pregnancies.
Researchers at Tokyo Institute of Technology and the Weizmann Institute of Science identify a means of controlling biological processes that could help treatments for immune disease, neurological disorders and cancer.
(PhysOrg.com) -- Australian scientists have contributed to an important international discovery, which could play a critical role in the future treatment of cancers and autoimmune diseases.
Researchers from across multiple disciplines at NYU Langone Medical Center created a new protein molecule derived from the growth factor progranulin may provide the basis for new therapies in inflammatory diseases, such as ...
Scientists have blocked harmful immune cells from entering the brain in mice with a condition similar to multiple sclerosis (MS).
Mount Sinai researchers have discovered that microglia, the immune cells that reside in the brain, have a unique origin and are formed shortly after conception. It was previously thought that microglia originated at the ...
(PhysOrg.com) -- A recently identified immune cell that directs other cells to fight infection plays a critical role in regulating the immune system in both health and disease. Researchers from the University of Pennsylvania ...


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I want to losslessly rotate all JPEGs that have been shot in portrait mode and remove the rotate flag, so iPhoto doesn't stops rotating them. Any easy way to do that?
Found it:
You must have both jhead and jpegtran installed. jhead can be found here (OSX binary available). jpegtran can be found here (download the source and compile it using


text:
We found 1 that has recently dropped in price.
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2002 Mazda Protege5 Wagon 4dr Wagon (2.0L 4cyl 5M)
Mazda wasn't even on my list of cars I
considered when I was shopping for one.
However, the Mazda dealership was next
to the Mitsubishi one, so I stopped
there... I test drove the Civic, Focus,
Eclipse, Tiburon et al., but there was
no contest. The handling of the vehicle
is in a class of its own. The ride is
firm, precise, sporty. The interior is
very attractive, all the materials look
and feel very high-class, no cheap
plastic. Nice versatility with the form
factor. Has needed nothing except for
regular oil changes. Standard
maintenance very cheap. Seats four
adults comfortably.
Handling, steering wheel and interior.
Adjusted gearing for the manual. It's
running too high RPMs at highway
speeds. Offer engine immobilizer as an
option. More compartments in trunk,
slightly better rear seat design for
I've had the P5 for about 5 months now
and really think it is a great little
car. The engine is peppy and with the 5
speed is quick enough for most city and
highway driving. Gas mileage is okay
(around 27 mpg mixed driving). We have
the sunroof and roof rack on and have
never noticed the so called whistle
like some others have, even going above
75 mph. We really like the room this
car has. It is surprising just how much
stuff can fit in the cargo area
(similar to the Protege Sedan trunk in
terms of surprise). We also liked the
fact that we could put two car seats in
with our seats back and travel in
comfort for a 6 hour drive.
Styling of car is great. We get a lot
of compliments about how nice it looks
in black. Many people can't believe its
a Mazda, others say Zoom Zoom when they
see it.
Better stock tires for locations that
experience winters with snow. The tires
are sport tires that are great on dry
road, but a little slippery on wet and
snowy roads.
Bought the 2003 P5 last month after 6
months of research. It's exactly what
this single gal needs! Got rid of a
Miata and Saab for my Protege5 (loaded,
w/ leather, roof, 6-disc the works) and
couldn't be happier with the price,
performance, and handling. Highly
recommend this versatile, sporty, and
(gasp!) economical ride!!
funnest car i've driven. more
practicle that i would've thought.
antilock brakes, sunroof, front & side-
airbags, four-wheel disk brakes, and
quick. handling is incredible.
more hp for better acceleration at
higher speeds.
I love the P5. Great tuner car with
above average handling, priced to buy
and great styling. A+
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14 Trade Fairs in Portugal from November 2017
Exhibition calendar with 14 trade fairs from November 2017 in Portugal sorted by exhibition date. Most trade fairs in Portugal take place in Leca da Palmeira with 7 trade fairs. The 3 most common types of fairs in Portugal are Gems and Jewellery Trade Fairs & Exhibitions, Furniture Trade Shows and Timber Industry & Forest Trade Shows
International exhibition for the construction industry
professional visitors and general public
Exponor - Feira Internacional do Porto Leca da Palmeira, Portugal
Alimentaria & Horexpo
International trade fair for food, restaurant, food and beverage industry
professional visitors only
Feira Internacional de Lisboa Lisbon, Portugal
Schedule of trade fairs with current 14 trade fairs in Portugal. Of course also other interesting trade fairs can take place in Portugal, which are not yet known to us. If you know trade fairs in Portugal that are not listed, you can transmit them to us via announce new trade fair Our team will update them in a timely manner to promote these fairs as well. Trade fairs with expired dates or trade fair dates yet unknown to us can be found at the end of the above list, labelled with the button "report new date of event".


text:
Small hop-diameter sparse spanners for doubling metrics
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T-H. Hubert Chan
Carnegie Mellon University, Pittsburgh, PA
Anupam Gupta
Carnegie Mellon University, Pittsburgh, PA
Published in:
· Proceeding
SODA '06 Proceedings of the seventeenth annual ACM-SIAM symposium on Discrete algorithm
Pages 70-78
Society for Industrial and Applied Mathematics
Philadelphia, PA
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Small hop-diameter sparse spanners for doubling metrics
A spanner or wrench is a tool used to provide grip and mechanical advantage in applying torque to turn objects¿usually rotary fasteners, such as nuts and bolts¿or keep them from turning. In British English, spanner is the standard term. The most common shapes are called open-ended spanner and ring spanner. The term wrench refers to a type of adjustable spanner. In American English, wrench is the standard term. The very most common shapes are called open-end wrench and box-end wrench.
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Ackermann function
In computability theory, the Ackermann function, named after Wilhelm Ackermann, is one of the simplest and earliest-discovered examples of a total computable function that is not primitive recursive. All primitive recursive functions are total and computable, but the Ackermann function illustrates that not all total computable functions are primitive recursive.
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Not to be confused with ¿ or ¿. For, E# redirects here. For the musical note, see E¿ (musical note). For the mathematical constant, see e (mathematical constant). For other uses, see E (disambiguation). E ISO basic Latin alphabet Aa Bb Cc Dd Ee Ff Gg Hh Ii Jj Kk Ll Mm Nn Oo Pp Qq Rr Ss Tt Uu Vv Ww Xx Yy Zz E is a vowel and the fifth letter in the ISO basic Latin alphabet.
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Metric space
In mathematics, a metric space is a set where a notion of distance between elements of the set is defined. The metric space which most closely corresponds to our intuitive understanding of space is the 3-dimensional Euclidean space. In fact, the notion of "metric" is a generalization of the Euclidean metric arising from the four long-known properties of the Euclidean distance.
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An asterisk is a typographical symbol or glyph. It is so called because it resembles a conventional image of a star. Computer scientists and mathematicians often pronounce it as star (as, for example, in the A* search algorithm or C*-algebra). In English, an asterisk is usually five-pointed in sans-serif typefaces, six-pointed in serif typefaces, and six- or eight-pointed when handwritten. It can be used to censor vulgar words or objectionable text.
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M is the thirteenth letter of the ISO basic Latin alphabet. It is used to represent one thousand in Roman numerals.
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Inverse function
In mathematics, an inverse function is a function that undoes another function: If an input x into the function ¿ produces an output y, then putting y into the inverse function g produces the output x, and vice versa. i.e. , ¿(x)=y, and g(y)=x. More directly, g(¿)=x, meaning g(x) composed with ¿(x) leaves x unchanged.
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In geometry, a diameter of a circle is any straight line segment that passes through the center of the circle and whose endpoints are on the circle. The diameters are the longest chords of the circle. The word "diameter" derives from Greek ¿¿¿¿¿¿¿¿¿ (diametros), "diagonal of a circle", from ¿¿¿- (dia-), "across, through" + ¿¿¿¿¿¿ (metron), "a measure"). In more modern usage, the length of a diameter is also called the diameter.
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Are You on the Same Page as Your Customers?
Leave it to kids to remind us that that we need really good listening skills.
Our young neighbor Jack, who's all of 7, came biking by our house early Saturday morning. "We're leaving for the Cape in about an hour," he yelled over, with evident glee, "and we're staying for a really long time!"
"Wow, that's great!" says I. "How long?"
"Till Monday!" says he.
Ha! I was expecting him to say, "The whole summer!" (Or at least a week or two.)
The joke was on me. But Jack taught me a couple of things we can all apply to business.
First of all, when a print customer says she needs something printed ASAP or even "right away," you need clarification. For all you know, she's thinking same day or next day—or maybe to her, next week is fine. If you don't ask, you'll assume it means what you think it means. Wrong!
Likewise, when you're discussing delivery dates for jobs or proofs, sometimes the date itself can mean different things to different people. "Friday" might mean end of day to you (5 p.m. your time), but a client could be thinking morning or early afternoon, so she can check out her samples or distribute some to her team.
Call it a misunderstanding or plain-old miscommunication, but whatever you call it, it can create real headaches for both you and your customer.
I remember the time when a critical direct mail campaign was scheduled through my printer. The company handled the mailing as well as the printing. I'd received the postage estimate earlier (natch) and, thinking everything was hunky dory, calmly waited for the mailing to take place within the week.
A few days later, I called my print rep to make sure everything was copacetic.
You may know Margie as the producer of the annual Print & Media Conference. Although she's exited the event production business, she's still publishing her Print Tips newsletter. She looks forward to helping companies create and style all of their content so their potential customers sit up and take notice. For details and to sign up for her Print Tips and new marketing blog, visit www.margiedana.com or e-mail Margie at email@example.com.
I have to say that is a great stance. I totally agree with it all. Its funny that you mentioned that last statement. I end every email or conversation on the phone with this " if there are any questions or if i can help in any way please don't hesitate to ask." What do you think?
Robert Bloecker
At my company in Vegas, we do a ton of convention printing, and we have had to actually come up with a set procedure where "timeframes" are involved. When is your show? When do you arrive? What hotel are you staying at? When can we have files? When are you available for proofing? You get the idea. As a las vegas printing company, http://www.rapidcolor.com has had to completely make "time" a integral part of our sales process, and we get more and more customer feedback about how great and professional it is. Customers can tell we are used to dealing with Convention printing situations, and thank us for our professionalism.
"And how about ending every critical conversation about planning a project with this: "Do you have any questions at all for me? Don't hesitate to call or email me if you do."
In all my years in this business, I don't recall any sales rep saying something like that to me."
That's so very sad…


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Ozy and Millie by Dana Simpson
- May 01, 2013
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Hmm. wisdom is explaining things as they are, not gibberish like "If the beer can is empty, the man was drunk in Chicago"
Here's some line from one of the most wise but stupid persons ever on the planet,
"13 I also saw under the sun this example of wisdom that greatly impressed me: 14 There was once a small city with only a few people in it. And a powerful king came against it, surrounded it and built huge siege works against it. 15 Now there lived in that city a man poor but wise, and he saved the city by his wisdom. But nobody remembered that poor man. 16 So I said, "Wisdom is better than strength." But the poor man's wisdom is despised, and his words are no longer heeded."
Learn, then unlearn… story of my life. Every time I read one fact, someone else comes along to refute it.
I don't see how this is any different than attending school and then coming home.
When your school IS home, there's no coming home to assist the unlearning.
At the end, of all what you learn in school, you only use 10% during he rest of your life, thanx John Dewey, you were a real stupid.
Nature of fact. It exists independent of unbelief. I've seen 2+2=5 often enough, but never juggled enough to change the real answer no matter what I believed.
You must always be ready to lean the new and discard the old.
"And how is education supposed to make me feel smarter? Besides, every time I learn something new, it pushes some old stuff out of my brain." – Homer Simpson
Homer has that crayon stuck up his nose pressing on his forebrain. Take it out and his IQ rises about 20 points. He did once but stuck it back in.
- Refresh Comments.
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My Work
Meadow Rue Merrill is an award-winning writer, contributing magazine editor and mom of six–including Ruth, who was adopted from Uganda. Meadow doesn't wear a clerical collar. She didn't graduate from seminary, but she believes that God is intimately involved in her everyday life, an experience she shares in her weekly newspaper column, "Faith Notes." Please subscribe to connect!
Redeeming Ruth
Ruth was 16-months old when she arrived in Maine from an orphanage in Uganda. She couldn't sit up, roll over or lift her head. In seven years Ruth never spoke a word, yet her life spoke volumes about the importance of family, the meaning of faith, and the power of love. "Redeeming Ruth" is her story.
Faith Notes: Words that Encourage
Life is often rough, mine included. Some days seem designed to crush your hopes and dreams. Faith Notes are brief, Scripture-inspired meditations that are meant to encourage. Each Monday I offer a new reflection based on what God is doing in my everyday life. You can also find Faith Notes on Fridays in The Times Record. Or subscribe to have it delivered to your in-box.
Bringing lasting change
Sometimes it feels that we can do little to stop the violence and hatred. Whether in our own country or far away, there is so much strife and misunderstanding, such fear. It is easy to become paralyzed. How can we, with our little efforts, bring lasting change?read more
Finding the kindness to care
I wasn't going to read the news reports on the murders at the Bangladeshi café. I was scanning the paper last Sunday morning when I saw the headline on the siege at the Dhaka bakery and passed right over it, not wanting to see one more bloody, terrifying image of pure hate. But the horror was so overwhelming, I found myself reading anyway.read more
Amid heartbreak, a cry for justice
It is a terrible time, when you have to talk to your children about what to do if they are confronted with a shooter. When you discover that another depraved, hate-fueled attack has shattered lives again, here in Florida or in a Nigerian village or the battered towns of Syria.read more
Perseverance, when you want to quit
On a recent Saturday, I caught a TED Radio Hour talk on NPR with Diana Nyad, the first person to swim 111 miles non-stop from Cuba to Florida. For the next quarter-hour, I was spell-bound as Nyad shared her journey of perseverance, overcoming multiple failures, physical challenges, and pain so severe that jellyfish stings caused her body to go into convulsions.read more
Prayer is like this…
After a decade of working to write a memoir about how God changed our lives through our daughter, Ruth, it seemed that I had come to the end of everything. I had already done everything in my power to see it published and could do no more. My best efforts to find a publisher who shared my family's vision of helping other children through Ruth's story had failed. Miserably.read more
What if it didn't depend on you?
Ever claim to believe one thing only to discover that you are in fact practicing a completely contrary conviction? Imagine a person who tells everyone how important it is to eat natural, whole foods while secretly binging on overly processed, chemically altered garbage. Yep, that was me.
Not the food so much, although I admit to an unholy addiction to vanilla ice cream, but the dualism of believing one thing and practicing another. While claiming that my life wholly depends upon the goodness, grace and supremacy of God, I have, simultaneously been acting as if it all depends on me.read more


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Professional Cleaners
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Our "Mighty Maids" will clean your San Diego home or business and leave it looking spotless!
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Garboldisham CricketForce - Young members of Garboldisham Cricket Club give the ground's fence (and themselves) a good coat of paint
Garboldisham CricketForce - Even the youngest got 'raked' in to CricketForce at Garboldisham CC in Norfolk!
Bloxwich CC NatWest CricketForce - Another team that performed some great CricketForce deeds - this time at Bloxwich CC.
Bloxwich CC NatWest CricketForce - A 15 ton HGV and Bobcat Digger at Bloxwich CC helping do the heavy work to turn a tennis court into an outdoor nets area.
Harborne CricketForce - ECB chief executive David Collier and England spinner Ashley Giles prepare to get involved with the work at Harborne CC.
Harborne CricketForce - Ashley Giles dons overalls and begins painting the ceiling in the clubhouse at Harborne CC.
Harborne CricketForce - Ashley Giles proves he is a true all-rounder as he heads outside to help with the sanding.
Harborne CricketForce - Some of the many volunteers at Harborne CC pose for photos with Ashley Giles and ECB chief executive David Collier.
NatWest CricketForce Reed CC - Up on the roof: volunteers do some general repairs at Reed CC in Hertfordshire as part of thier CricketForce event.
Blyth CC NatWest CricketForce Chris Read4 - Blyth CC in Nottinghamshire arranged their own helicopter to fly in England and Notts keeper Chris Read as the star attraction of their CricketForce event - an amazing achievement! © Worksop Guardian


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I am not very good at coding I started off with HTML then stopped and come back and all this new coding and Im just so bewildered.
Let me first tell you what I would like to do.
I want to have a membership website, where there are three selections.
1. Free Matches - still need to be registered
2. Premium Account- i.e. $20 a year
3. Battles where you must pay a virtual credit in which you must pay with real money.
Ive done some research and paypal has an Xpress Checkout that allows API of virtual credit system.
Though I'm not sure how it would recieve my users exact account "bank"
Also to be honest I'm not even sure how to be able to get users to sign up with there own information, then that information is processed into a profile, with a selection for BANK
Can anyone help me please. I can offer some more explanation if need be.


text:
This is Waylon Lewis' weekly editor's letter, that appears first in our free email newsletter, which gathers the 10 best blogs/videos/events/articles each week. Overwhelmed by twitter, Facebook? Want a pretty, professionally designed email once a week, free? Subscribe.
Peace on Earth! Hold that thought.
"Do not rejoice when your enemies fall, and do not let your heart be glad when they stumble. ~ Proverbs 24:17
"In the Shambhala [Buddhist] warrior tradition, we say you should only have to kill an enemy once every thousand years." ~ Chogyam Trungpa
We Americans must all remember: when we pray for revenge, or war, or victory, we inadvertently pray for other things, too.
While our celebrations in America, last night, have been joyous, and bipartisan, and have not vilified Muslims (thank God), there is still the shadow of an uncomfortable visual parallel to those images that fill us with so much fear and hate: young men in Iran, etc., celebrating by burning our flag, chanting Death to America.
Death is not sport.
And so while I'm grateful Osama is gone—his absence gives me hope for peace, for safety of innocents civilians—I do not know that we should indulge in the satisfaction of sated hatred.
That said…
"I've never wished a man dead, but I have read some obituaries with great pleasure." ~ Mark Twain
hot on elephant
A Letter to my Children: You do not come from a Broken Home. These People are Rare Gems—Keep Them, Fight for Them, don't Give Up on Them. Mom, can I Call her Mom, Too? My Marriage had to End—for my Life to Begin. The Day I Stopped Running. Why your Yoga Goals are (Probably) Irrelevant, if not Downright Dangerous. My Favorite Part of Sex is You. {Adult} Dear Woman in the White Car at Margaritas Mexican Grill in West Memphis, Arkansas on July 15th, 2012. Overcoming the Storm by Becoming the Storm. A Toast to PTSD: The Solution Starts with One Question.


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text: This family-oriented community event will celebrate the 10-year anniversary of the research institute, raise awareness of the research institute and show some of the breakthroughs being made there. It will be a street fair with many activities for the community, especially those with kids. Some of the activities planned include interactive stations where kids and adults can learn about science and research; a bike helmet giveaway, booster seat education and tips for healthy eating; a research institute welcome center open house; and food trucks.


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Wednesday January 23, 2008 4:29 pm
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Conceived and designed the experiments: JDK MRH BBJ BM KFF. Performed the experiments: JDK BBJ BM KFF. Analyzed the data: JDK MRH BM KFF. Contributed reagents/materials/analysis tools: RMN. Wrote the paper: JDK KFF.
Multiple sclerosis (MS) is a demyelinating disease of unknown origin that affects the central nervous system of an estimated 400,000 Americans. GBV-C or hepatitis G is a flavivirus that is found in the serum of 1–2% of blood donors. It was originally associated with hepatitis, but is now believed to be a relatively non-pathogenic lymphotropic virus. Fifty frozen specimens from the brains of deceased persons affected by MS were obtained along with 15 normal control brain specimens. RNA was extracted and ribosomal RNAs were depleted before sequencing on the Illumina GAII. These 36 bp reads were compared with a non-redundant database derived from the 600,000+ viral sequences in GenBank organized into 4080 taxa. An individual read successfully aligned to the viral database was considered to be a "hit". Normalized MS specimen hit rates for each viral taxon were compared to the distribution of hits in the normal controls. Seventeen MS and 11 control brain extracts were sequenced, yielding 4–10 million sequences ("reads") each. Over-representation of sequence from at least one of 12 viral taxa was observed in 7 of the 17 MS samples. Sequences resembling other viruses previously implicated in the pathogenesis of MS were not significantly enriched in any of the diseased brain specimens. Sequences from GB virus C (GBV-C), a flavivirus not previously isolated from brain, were enriched in one of the MS samples. GBV-C in this brain specimen was confirmed by specific amplification in this single MS brain specimen, but not in the 30 other MS brain samples available. The entire 9.4 kb sequence of this GBV-C isolate is reported here. This study shows the feasibility of deep sequencing for the detection of occult viral infections in the brains of deceased persons with MS. The first isolation of GBV-C from human brain is reported here.
Multiple sclerosis (MS) is a chronic demyelinating disease of unknown cause, which affects the brain and spinal cord of about 400,000 individuals in the U.S. A number of viral infections of the CNS can lead to demyelination, including distemper (dogs), measles (SSPE, humans), and influenza (humans). [1] Viruses have long been suspected as causative agents in MS based on the epidemiology of the disease including geographic patterns, isolated outbreaks, and migration studies. [2], [3], [4], [5] Novel viruses that cause human disease continue to be discovered using molecular techniques including hepatitis C (1989), corona virus NL63 (2004), bocavirus (2005), and rhinovirus C group(2007). [6], [7], [8], [9] New human polyoma and arenaviruses, identified by high-throughput or "deep" sequencing of pathologic specimens, were recently discovered as causes of serious human diseases(2008). [10], [11]
To date most studies into the possible viral etiology of MS have focused on human DNA viruses. Unfortunately, because DNA viruses can become latent or quiescent within the CNS, it is difficult to discern whether latent, commensal viruses associated with MS lesions (e.g. HHV-6 and EBV) are causative or just opportunistic (i.e. "along for the ride"). To avoid the identification of latent viruses that are not transcriptionally active, the present investigation focused on viral RNA – both genomic (from RNA viruses) and message (from either RNA or DNA viruses).
Deep sequencing offers a new approach for the discovery of pathogens. In contrast to PCR, this approach allows a relatively unbiased survey of nucleic acid sequences found within a sample. Deep sequencing provides millions of short sequences less than 100 bp in length. The completion of the Human Genome Project allows subtraction of human sequences from the dataset resulting in a large number of plausibly-nonhuman sequences that can then be compared to existing databases such as GenBank. This technique was recently employed to discover novel picornaviruses linked to respiratory illness and diarrhea in children. [12], [13]
GB virus-C (GBV-C or hepatitis G virus) is a human flavivirus originally isolated from a surgeon (with the initials G.B.) who was experiencing acute hepatitis. [14] The GB viruses are approximately 10 kb in size and are most closely related to hepatitis C virus. Other flaviviruses include dengue viruses, West Nile virus, yellow fever virus, and the tick-borne encephalitis viruses. In addition to humans, GBV-A and GBV-B infect monkeys, GBV-C infects chimpanzees. The recently described GBV-D infects only bats. [15], [16] Animal inoculations suggested that GBV-C might be a cause of hepatitis, but subsequent human observations did not confirm this. GBV-C is known to be lymphotropic and has been found in human serum, muscle, lymphoid tissue, spleen, kidney and liver, but not brain (0 of 8 specimens) or spinal fluid (0 of 17 specimens). [17], [18], [19] Surveys show that active viral replication occurs in the blood in 1–2% of human blood donors while about 5% of the general population is seropositive. [20] Viral replication can persist for years at high titer (up to 50 million copies per ml blood). This virus is believed to be nonpathogenic, but has been associated with non-Hodgkin's lymphoma. [21], [22], [23] Resolution of GBV-C infection is often accompanied by antibody production against the envelope (E2) protein. GBV-C infection appears to induce antibodies that cross-neutralize HIV, probably explaining the observed survival benefit of persons infected with both viruses over those with HIV alone. [24]
We report the results of deep sequencing of diseased brain tissue (plaque) taken from deceased patients with multiple sclerosis. This method identified one occult viral infection with GBV-C in a patient who died with primary progressive MS.
One hundred mg pieces of cryopreserved white matter from MS plaques or control specimens were obtained from the Human Brain and Spinal Fluid Resource Center, West Los Angeles VA Medical Center and from the Rocky Mountain MS Center, University of Colorado, Denver. These specimens were collected after death and were either fresh frozen or snap frozen in liquid nitrogen. This research was submitted to the University of Utah Health Sciences IRB and, since it was performed on deidentified pathologic material, was found to be exempt from review and oversight.
DNase treated RNA was extracted from the tissue sections using Qiagen RNA-lipid kits (Valencia, CA) on dry ice, under laminar airflow. Purified RNA was quantified on a Thermo Scientific Nanodrop spectrophotometer (Waltham, MA). Samples with absorbance (A260/A280) ratio <1.8 were rejected and re-extracted. Fifty ng of RNA from each sample was submitted the Huntsman Cancer Institute (HCI) Microarray Core Facility lab for analysis on an Agilent 2100 Bioanalyzer Nanochip (Agilent Technologies, USA). The extracted RNA samples were evaluated for their distribution of RNA sizes, their relative abundances, the height and relative ratio of the 28S and 18S rRNA peaks, and for RNA integrity (RIN) as determined by the Agilent software. Each sample was evaluated by an experienced technician and then independently by one of the investigators. Samples with an unfavorable electrophoresis trace or RIN were not sequenced.
To enhance the detection of non-human sequences, RNA samples that passed the quality control step above were subjected to rRNA removal using the RiboMinus kit (Invitrogen Inc., Carlsbad, CA). Recovered RNA (post rRNA removal) was again quantified and subjected to Bioanalyzer analysis.
RNA samples that passed the RNA quality control were submitted to the Huntsman Cancer Institute Microarray Core Facility for reverse transcription, cDNA library preparation, and high-throughput sequencing. Datasets of 36 bp sequences, or reads, were obtained from each brain sample. Low complexity (LZW compressibility >0.4) reads, reads with homopolymeric stretches longer that 18 bases and those with Bustard quality scores ≤5 in more than 13 positions were excluded from the analysis. The remaining reads are defined as "high quality reads." Reads which perfectly matched UCSC build 18 of the human genome, as judged by Bowtie alignments, were excluded from the analysis. Weaker matches to mammalian rRNA transcripts were detected and removed from the data set using MegaBLAST (word size=12) with a database of 45S pre-rRNA and 28S sequences (gis: 1261918 and 225637499). The remaining high quality, nonhuman, nonribosomal reads were defined as "screened reads." Comparative enrichment of viral sequence was then determined using these filtered sequences (screened reads).
A non-redundant database of viral sequences was built to reduce noise from highly oversampled taxa in the public databases. This nonredundant database is 98 megabases in size organized into 4080 viral taxa. All viral sequence in GenBank was obtained (more than 600,000 records). The longest sequence from each viral taxa was extracted from the input sequence set and placed in the non-redundant output set. All subsequences longer than 23 bases in the input that were compared to the sequence that was just extracted. Identities were removed from the input by replacing the identical subsequence with X's. The longest remaining sequence, thus screened, was then extracted from the input, placed in the non-redundant output and used to screen the remaining input sequences. The process was repeated until no unscreened subsequences longer than 49 bases remained in the input. The resulting database, NR_ViroBank is available online (http://fischer-lab.path.utah.edu/data/GBV-C).
MegaBLAST was used to align the brain sequences to NR_ViroBank using a low-stringency protocol. A word size of 16 was used, sequence filtering was disabled, and sequence-virus pairs with expect values ≤1.0 were considered in further analysis. The hit-rate (HRT-S) of a sample to a viral taxon was calculated using Equation 1 where the number of alignments to a given taxon (AT-S) is normalized by the number of filtered nonribosomal sequences obtained for the specimen (RS):
The mean and standard deviation of the normalized hit rates in the control specimens were determined for each viral taxon. The distribution of control hits was examined for normality. Taxa with non-normally distributed control hit rates were excluded from further analysis. The hit rates of each individual MS sample were then compared to the control hit-rate distribution for each taxon. Using the python programming language and scipy, the Bonferroni corrected Student's t-Test was used to quantify the statistical significance of any overrepresentations within each MS sample. Cluster and Java Treeview were used to generate an unsupervised cluster of significant sample-taxa p-value pairs. [25], [26]
PCR primers were designed from 4 regions of the GBV-C genome based on the deep sequencing results. Reverse transcription was performed using independent forward and reverse gene specific priming. Primers were selected from the 5′-NTR, E2, and NS3 gene regions based on Souza et. al. with modifications as suggested by the deep sequencing results. [27] Primer sequences (5′-3′) employed were:
Quantitation of GBV-C was performed using the following (5′-NTR) primer and probe sequences:
SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase (Invitrogen, Carlsbad, CA) was used with running conditions: 50°C 20 min, 95°C 2 min, 40 cycles of 95°C 15 sec, 58°C one minute. A standard curve was generated using log dilutions of synthetic standard GBV-C RNA (kindly provided by the Stapleton laboratory at the University of Iowa).
Three anti-GBV-C antibody preparations and controls were kindly provided by the Jack Stapleton laboratory at the University of Iowa. These included pre- and post-immune rabbit polyclonal antiserum against GBV-C E2 protein [24], mouse monoclonal anti-E2 #7067 [24], and mouse monoclonal 1C4 [28], plus control mouse IgG1 (Vector Laboratories, Burlingame, CA). Frozen MS and control brain tissues were thawed and fixed in 4% paraformaldehyde. Five micron paraffinized sections were used. Antigen retrieval was performed (Vector product H-3300). The tissues were penetrated with Triton-X and endogenous peroxidase was quenched. Sections were blocked with 5% normal goat serum (Vector product S-1000). Appropriate goat anti-rabbit or goat-anti-mouse IgG secondary antibodies (Santa Cruz Biotechnology, products sc-2004 and sc-2060) were diluted 1500–1000 as recommended by the manufacturer. Signal was developed with NovaRed (Vector product SK-4800).
Thirty-two specific primers to the MS-6 GBV-C isolate were designed using the GAII sequences that were highly homologous to known GBV-C isolates. Overlapping amplicons were obtained using RT-PCR with freshly extracted total RNA from sample 3840. Amplicons were TOPO-cloned and sequenced using an ABI 3730 sequencer. The Sanger sequences were processed and assembled using the phred, phrap and consed programs using a known GBV-C scaffold (gi: 1666805). [29], [30] The GAII reads were added to this intermediate assembly using consed.
Fifty frozen MS plaque and 15 control, undiseased brain specimens were extracted. Among these, 17 MS and 11 control brain specimens were selected for sequencing based on favorable RNA quality profiles. These brain specimens were collected between the years 1983 and 2004. They were kept frozen at −70 degrees until being subjected to RNA extraction in our laboratory. Characteristics of the sequenced brain specimens are shown in Table S1. The post-mortem intervals between the MS and control specimens were similar: 12.9±2.2 vs. 11.5±1.6 hours (p=0.64 by unpaired t-testing). The MS subjects were younger than the controls at the time of specimen collection: a median of 55 vs. 69 years, (p=0.01 by unpaired t-testing). The proportion of specimens obtained from each of the two brain banks was similar between the MS and control groups (p=0.7 by Fisher's Exact test). The 17 sequenced MS specimens came from deceased patients that carried the diagnoses of primary progressive MS (3), chronic progressive multiple sclerosis (5), secondary progressive MS (5), relapsing-remitting MS (2), or multiple sclerosis, subtype not specified (2).
High-throughput sequencing on the Illumina GAII yielded 4–24 million 36 bp sequences ("reads") from each of the 28 brain specimens (17 MS, 11 normal controls). These reads are designated as "high quality reads" in Table S1. These sequences represent cellular RNA, not DNA, and therefore correspond either to viral genomes or to actively transcribed genes, both human and nonhuman, present in the specimen. Human and ribosomal reads were subtracted from the dataset, providing 0.9–7.4 million screened reads for analysis. There were no significant differences in the number of high quality reads or screened reads between the MS and control specimens (p>0.5 by Mann-Whitney testing).
Viral alignments were performed on the sequences derived from each of the MS and control brain specimens. Hit rates were calculated for each taxon represented in the nonredundant viral database (N=4080). The mean and standard deviation of the hit rates in the control specimens were determined for each taxon. Only 109 out of 7947 (1.4%) viral taxa showed a non-normal distribution of control hit rates. These were removed from further consideration. Bacteriophage and plant virus taxa were excluded from further analysis due to less biologic significance compared with human and animal viruses. Retroviruses were reserved for later analysis due to the ubiquitous expression of human endogenous retroviruses (HERVS) in human specimens. Hits were observed to 2153 remaining taxa. The hit rates to each taxon for each MS sample were then compared to the control hit rate distribution for that taxon. Student's t-Test corrected for multiple comparisons using the Bonferroni method was used to quantify the statistical significance of any apparent over-representation observed in the MS samples. The 12 taxa with a least one MS sample with a over-representation more significant than p=0.05 were placed into a hierarchical cluster (Figure 1). Over-representation in at least one taxon was observed in 7 of the 17 MS samples.
The 36 bp reads from the Illumina sequencing are not likely to identify any viruses unambiguously. Overrepresented viral taxa (shown in Figure 1) had all the deep sequencing reads from the implicated sample aligned to a database containing all GenBank sequences for the taxon of interest. The reads were then joined into contiguous regions (contigs) using SSAKE, a short read assembler. [31] Contigs generated by SSAKE were then aligned back to the taxon specific database. These newly assembled contigs were then aligned to both host and taxon specific viral databases again. Contigs that had improved alignment quality (compared to single reads) to the candidate viral sequences and worse alignment to human sequences were used to design primers for virus specific RT-PCR. The results of this analysis are shown in Table 1. This strategy identified GBV-C as a promising candidate for PCR follow up in a single MS brain sample.
Table 2 shows the analysis of deep sequencing results for viral taxa previously implicated in the pathogenesis of MS. Overall, the MS specimen hit rate was relatively high for the human herpesviruses compared to other viral taxa, probably due to the detection of RNA expressed during latency. It is also possible that these higher hit rates are driven by short sequences derived from abundant human transcripts that align to the herpesvirus taxa. However, none of the human herpesvirus nor measles virus hit rates differed significantly between the MS and control distributions.
One subject who died with primary-progressive MS had >1000 36 bp sequences detected that mapped to GBV-C virus (hepatitis G), a human flavivirus not known to cause any persistent disease and never before detected in human brain. [14], [17], [18] This brain specimen was investigated further. An initial attempt to amplify GBV-C from this specimen using published primers was unsuccessful, later determined to be due to sequence variation of the isolate. [27] PCR primers for 3 viral regions (5′-NTR, E2, and NS3) were designed from the contigs assembled from the Illumina sequencing data on this particular subject. RT-PCR amplification of these regions revealed amplicons of the expected sizes, approximately 300 bp (Figure 2). Sequencing of the amplicons showed 96–99% identity to >100 known GBV-C sequences. Quantitative rtPCR of this brain sample showed that there were between 1.7×106 and 2.4×106 viral genome copies per gram of tissue. Interrogation of CSF from this subject showed 1.1×106 viral genome copies/ml. Blood from this subject was not available for analysis. Anti-GBV-C antibodies were not detected in CSF from this subject (personal communication from Dr. Jack Stapleton).
Specific RT-PCR amplification of 31 affected (MS) and 23 unaffected (control) brain samples was performed with each of the 3 GBV-C primer sets testing for both positive and negative strand viral RNA, using the existing GBV-C positive sample as a positive control. Both strands of GBV-C RNA were detected only in subject MS-6, both before and after ribosomal removal, and not in any of the other 53 brain RNA specimens studied. Twenty MS and seven control CSF samples were interrogated with 3 separate GBV-C specific primers (NTR-1, E2, and NS3). The MS-6 CSF sample was used as a positive control. GBV-C was amplified from none of these other 27 CSF samples.
The recovered sequence of GBV-C UU1 contained a open reading frame of 2842 codons, containing the structural and nonstructural genes of the virus (Figure 3). GBV-C UU1 was compared to 24 other genomic length isolates in GenBank. The isolate most similar (89.7%, nucleotide identity over the genome) to GBV-C UU1 was the East African isolate GBV-C (EA) (gi:1666805). [32] At the amino acid level the viral polyprotein was 97.7% identical to GBV-C (EA) (64 coding differences). The sequence of GBV-C UU1 has been deposited with GenBank (GB JN127373).
Immunohistochemistry with controls was attempted on GBV-C infected, MS afflicted brain (MS-6); other MS brain specimens where GBV-C was not detected by PCR; and several normal brain controls. Some anti-GBV-C dependent staining of neuronal cytoplasm was observed in both infected and uninfected brain sections with the polyclonal rabbit anti-GBV-C preparation. The monoclonal Ab preparations did not specifically detect GBV-C in any of the human brain tissues examined, including that from MS-6 (shown by molecular methods to contain GBV-C RNA).
This work demonstrates the feasibility of deep sequencing for the detection of occult viral infections in brain tissue. This method revealed several viral candidates. The presence of one of these candidates, GBV-C, was confirmed in the brain of one of the subjects who suffered and died with progressive MS. This virus has never been detected before in human brain tissue. It's relationship with the underlying disease, primary progressive multiple sclerosis, in this single person is not clear. RNA sequences from other viruses previously implicated in the pathogenesis of MS including EBV, CMV, HHV-6, and measles, were not significantly overrepresented in the diseased brain specimens compared with controls.
GBV-C or hepatitis G virus is currently believed to be nonpathogenic. However, this virus was originally isolated from a human who was suffering from acute hepatitis. [33], [34] Prior attempts to isolate GBV-C from the brain in infected subjects were unsuccessful. [18] Other flaviviruses certainly do infect the human central nervous system including West Nile virus, dengue [35], [36], tickborne encephalitis viruses, and Japanese encephalitis virus. Even hepatitis C virus has been isolated from diseased brain in a patient with fatal demyelinating disease. [37] The epidemiology of GBV-C infection is similar to that of MS itself where infection/disease is rare in children and much more common in women of childbearing age. [38] GBV-C is, in part, sexually transmitted. [39], [40], [41] The sex predominance of GBV-C infection is about 21 womenmen, again similar to MS. [42]
The presence of both positive (RNA genome) and negative (replication intermediate) strand flaviviral RNA in MS-6 suggests that the virus was actively replicating in the brain of this subject. Active replication is also supported by this subject's pathology report showing "active plaques" with a perivascular lymphocytic infiltrate (data not shown). The clinical history revealed onset of primary progressive MS beginning at age 44 in an otherwise healthy Caucasian woman. The disease was relentless causing the subject to use a wheelchair by age 55 and to become bed bound at age 60. Active replication GBV-C in the brain of MS-6 is also supported by the relatively high titer of virus observed in both tissue and CSF (1–2 million copies per ml) and the absence of anti-GBV-C antibodies in the CSF.
Actively replicating GBV-C in this subject's brain may or may not be causal. GBV-C may be found in the blood of humans for years after acute infection and it may be present at high titer – in the range of 108 copies per milliliter of blood. [14], [20] Resolution of GBV-C viremia usually coincides with the development of antibodies to the virus. GBV-C replicates primarily in peripheral blood mononuclear cells, including lymphocytes, so its presence in the brain of subject MS-6 may simply reflect penetration of the damaged blood-brain barrier by serum or virus-infected lymphocytes. [18] On the other hand, persistent GBV-C infection of the brain might have manifestations not previously described, such as progressive demyelination. Animal studies demonstrate that viral infections of the CNS can result in a demyelinating disease that is similar to MS. [43], [44], [45], [46], [47] The failure to specifically detect GBV-C by immunohistochemistry in brain sections from subject MS-6 was disappointing. This may be due to a relative absence of viral antigen in these tissues or the lack of suitable reagents for the detection of GBV-C antigen in fixed, paraffinized tissue. In any case, the lack of detectable GBV-C RNA from 30 other MS brain specimens suggests that this virus will not be isolated frequently from the brains of MS patients.
Deep sequencing is a powerful tool that has revealed new pathogens. This report shows the feasibility of this technique for the study of brain tissues affected by MS. A novel isolate of GBV-C was identified here in this manner. The presence of GBV-C in this brain specimen was confirmed by designing PCR primers from the sequencing results. The deep sequencing analysis in this study was based on the early Illumina GAII technology and limited by read length (36-base pairs) and sequencing from only a single end of the library inserts. The investigators believe that further improvements in sequencing technologies, such as longer reads and perhaps paired-end strategies, will significantly simplify the bioinformatics required for future clinical viral metagenomic studies.
Demographic, sequencing, and disease characteristics of the sequenced MS and control brain specimens. The source, age, sex, year of collection, post-mortem interval, and diseases associated with each specimen used for this study is shown here, along with the number of reads obtained.
The authors would like to acknowledge Drs. Jack Stapleton and James McLinden from the University of Iowa for GBV-C related advice, reagents, and CSF serology. Brian Dalley from Huntsman Cancer Institute Microarray Core Facility supervised the preparation of cDNA libraries and the Illumina sequencing. Theodore Wilson helped with the genome assembly.
Competing Interests: The authors have declared that no competing interests exist.
Funding: This investigation was supported by grants from the National Multiple Sclerosis Society. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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GREEN BAY, Wis. -- This is exactly what the Green Bay Packers wanted in the mid-1990s. Two decades later, they finally have the Dallas Cowboys at Lambeau Field in the playoffs.
Oh, what they -- and their fans -- would have given for this in 1993.
Or 1994.
Or 1995.
All three of those seasons ended with playoff losses to Troy Aikman, Michael Irvin, Emmitt Smith & Co. And all three of those playoff losses were at Texas Stadium.
It became both an obsession and a frustration for the Packers' organization and its fans. If the Packers were ever going to get to another Super Bowl, they were going to have to beat the Cowboys.
Even in faraway Charlotte, North Carolina, they understood. In the playoffs following the 1996 season, it looked like it might come down to the Packers and Cowboys once again in the NFC. While the Packers hosted the San Francisco 49ers in one divisional playoff game, the Carolina Panthers had the Cowboys in the other.
"I can remember them flashing to a bar in Wisconsin someplace, and all the fans were chanting 'We want the Cowboys,'" said Packers defensive coordinator Dom Capers, who was the Panthers' head coach at the time. "And somebody came in and said to me, 'Well, you disappointed the Packers fan,' because it became Carolina coming in here.
"Three years in a row they had gone down there and finally, the Packers had home-field advantage, and they wanted the Cowboys to have to come here."
No one in Green Bay will ever apologize for skirting the Cowboys on the way to Super Bowl XXXI -- the Packers beat Capers' Panthers 30-13 in the NFC Championship Game -- and there's no asterisk next to their championship, but the Packers will never know if they could have beaten Dallas in the '90s in the postseason.
"That was very frustrating to go down there all the time and lose to those guys," former Packers general manager Ron Wolf said this week. "Very frustrating."
Wolf conceded that the Cowboys "probably had better players." He called Aikman, Irvin and Smith "exceptional," but thought his quarterback, Brett Favre, canceled out Aikman. Likewise, he believed Reggie White was to the Packers' defense what Deion Sanders was to Dallas' (although Sanders played in only the 1995 playoffs). But Irvin and Smith, especially on the fast track in Texas, were too much.
"I always felt if we could ever get them on grass -- we were always playing down at their place, and they had the distinct advantage on AstroTurf," Wolf said. "It was one of those things where we were structured differently physically as a football team than they were. We were a grass team, and they were an AstroTurf team, not to diminish the quality of their players.
"When we did get them on the grass, we beat them."
They didn't just beat them. They destroyed them -- 45-17 in a 1997 regular-season game at Lambeau Field. By then, the Cowboys' dynasty had begun to crumble. They would go 13 years before they won another playoff game.
The Packers finally get the Cowboys in Green Bay for a playoff game Sunday in the NFC divisional round. Other than what they have heard or read about the Ice Bowl, the last playoff game between the teams at Lambeau Field, few of today's players have much knowledge of the playoff history between the two teams.
Packers receivers coach Edgar Bennett does. Bennett, a Packers running back from 1992 to '96, played in all three of those playoff losses in Dallas.
"It's always difficult going back to reliving some of those Dallas games," Bennett said this week. "You learn from those experiences, and I think we were able to get better."
The one that sticks out to him the most is the 1995 NFC title game.
"I just know that locker room following that game in '95, I know we were a different team after that moving forward," Bennett said. "We were a different team. For whatever reason, we were a different team, and that following year we ended up winning the Super Bowl."
But they never beat the Cowboys.
"People say that," Bennett said. "We didn't really care who we ended up playing because we felt like it was more about where we were at and what we're doing."


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92 reviews | 4.7 out of 5
i saw a review, a bad, how hard it is to set up, put child in, how it's only for little ones,,, well the lady that wrote that didn't probably have this product :)
I and everybody else in my family love it. It so easy to put it on and take it of and i'm 5'4". My parents are over 60 and they love it. I have two girls. At first i bought this for my little 5 month old but if you have kids you will know, i end up puting my 19 month old who weights 23lb in it to. I was shock, she went in and jump, swing and twisting, she had so much fun, and i got so happy. Normaly product say up to 25lb but you cant put child in when is 20lb, but this product don't lie, my 19 month old jump's a lot in it and she is 23lb. I realy recomend this product to any parent. This product help you if you need to do anything and it does not take space. At all. Thank you. Petra
my 3 month old loves to stand already so we put him in it and he loves the toys!
We decided to buy this product for our daughter because of it's price and it also looked good on the picture. We did not scrutinized the neither this one nor the other options. Nevertheless, it turned out to be a perfect decision made. Our daughter ( six months now ) loved it; she sometimes gets so tired jumping in it that she finally gets to sleep in it! The lights and sounds are a big plus. There are connections on the ropes so that u can attach additional toys on it. Definitely would recommend it!
My baby LOVES this! He jumps and jumps with the biggest smile on his face! the only downside is that the only doorway it work in is the one in our master bathroom. I wish there was a way to put it in the main living area so that we don't always hang out in the bathroom...
My daughter absolutely LOVES this! I wasn't sure at first if she'd actually jump in it, but she just "got it" a few days past 5 months, and she can't get enough! lol :-)
Usually, she whines when she needs to go to sleep, and I'll put her in bed. She likes the jump and go so much that I'll hear the spring for a bit, then not hear it, and she's asleep in it! lol :-D
Excellent value for the money!
It was easy to put together too, I didn't even need to look at the instructions.
I don't have anything bad to say about this at all, but a big THANK YOU to Evenflo for making such an awesome product!
my 7 month loves it
This jumper is SO great! We ordered it so that my six month old son, who isn't crawling yet, could get some energy out. He jumped in it for about an hour until he finally fell asleep- he was so worn out! I was worried that my son would be too big for it because he's got chubby legs and is already 20 pounds, but he fits just fine and it holds him just fine! It's so great and he has so much fun in it! The little music and lights toy is such a great bonus! I only wish that'd I'd gotten this months ago!
The product said it was for 4 months and older, but our son's (who is 20 weeks old) armpits do not come up to the top of the cloth pouch yet. We have only had it for 2 weeks and he is not jumping yet. When he has had a nap and is in a good mood, he enjoys pushing the buttons and making it play music. I wonder if this is distracting him from using his legs to learn to jump. It is hard to get him out of it with one person. His legs stick in the cloth pouch. I'm sure he will grow to love it.
I bought this for my 6 month old grandson. It was simple to use - already put together - just had to attach it to the door frame and shorten it a little. It has been fantastic. He immediately began bouncing in it and continues to love it. His siblings say he looks like he's dancing an Irish Jig. He loves to bounce and play with the attached toy. I would recommend it highly!!
I bought this for my grandson, who is 4 months old. He loves bouncing in this. I like the tray as an added safety feature so he doesn't hit the door frame when he bounces. I would recommend this product.
I was a little unsure of buying this at first because I read reviews about the spring squeaking and the music being too loud. Though they springs were squeaky at first it went away in a couple of days after it had been used. The spring just needed to be broken in. As for the music, it has too different noise levels, both of which I don't think are loud at all. My baby is fine with both. For a five month old he is on the longer end, at 27 inches but that was no problem because the door jumper adjusts super easy. You just need common sense and you'll see how it adjusts or just read the manual, which is not big at all, or look at the pictures. It's simple. I like how it has the toy in the tray. It spins and on one side it has a mirror so my son really likes spinning that as well as the lights on it. He wasnt sure about jumping and spinning in it, but after a week he got use to it and enjoys being in it longer now.The toy can be removed so you can replace the batteries. It takes 3 AAA batteries that do not come with it.
My 5 month old LOVES this! I can sit him in it and he'll just sit and jump forever!!! My baby is a pretty big boy (17 lbs) and his chubby little legs fit just fine in the leg holes with room to spare. We love it!
both me and my baby love everything about this product! He loves the toys and it is easy for him to bounce in. Its great because its portable and can take it room to room in our home. The only slight complaint I have is that the spring is a little squeaky when he jumps. I don't know if that is common or if it was just the one I bought. I would highly recommend it. Its a little pricier the some but worth the money.
We purchased this as my son did not like the activity center we bought and the jumpers like them are so expensive. It is a great product for the price, The set up is easy, I feel secure putting my son in it,and he absolutely loves it! He will jump for an hour strait and then get mad cuz he gets tired and doesn't want to get out!
I have a day care and have numerous babies in this throughout the day. It has held up wonderfully! The toy bar is great. It gets a hard workout and it has held up to the jerking and pulling. I have babies from 4 months to 11 months jumping in this. It is great for the pre-walking stages. I would recommend this for all moms.
While my 5-1/2 month old LOVES to jump in his jumper I have just a few issues with it...here are the pros and cons. Pros - The bright colors and battery operated toy that attaches to the seat are great. Baby loves to hit the buttons and watch the lights. It is also really nice that there is a bit of a protective circle around the kiddo so that they aren't running into the wall as they bounce all around. Baby can play for a good long while before getting tired of this game! The height adjuster is also easy to use and the entire thing was easy to assemble. Cons - When we received the product and began using the jumper it was sooooo noisy. The spring was super squeaky. A spraying of WD-40 cured the squeak for the most part but it still makes a little bit of a noise. Also, the nylon strip that is used to connect the spring to the clamp which attaches to the ceiling gets wound up as baby bounces and hops around in circles. Because the strap gets wound so tightly as the baby plays it now looks wrinkled and if I had it to do over again I would have purchased a jumper that let the seat spin independent of the strap. It looks kind of wrinkled and ratty. Overall once we took care of the noise issue I'd say it was a fairly good purchase. Especially since the little one loves it so much.
I bought this for my grandson(4 months old). The first time, I put him in it, his head rested on the side of the strap in the back of the seat. I was a little concerned, took him out and tried adjusting the straps on chair(3 straps connected, in a y shape at base of spring, to strap of door mount), it allowed some movement to the front and I put him back in. He played for a while and enjoyed the sounds and all the different toys. Next day, I purchased a seat belt shoulder cushion (Walmart Auto Dept. $4.00 +-) to put on back strap to ease my mind! I think he is a little small for this, at this time, but I can see in the past 3 weeks that he loves it! When I take him out of it and stand him, in my lap, all he does is jump up and down! Guess that says it all! Can't wait for a few more weeks! Seems durable but will not stay facing front of doorway(gives him some changing scenery!}Hope this helps you.
My son loves this jumper. I hang it on the doorway in our house and he stays entertained while I fold laundry, etc. I purchased this because of the added music and toys but honestly, my son mostly jumps on it without really using the music option. He'll play with it for awhile but then just continue jumping. I also like the bumper feature especially if you are using it in a narrow doorway. Obviously you need to keep a close eye on our child while they are jumping. My son likes to hang his arms out to the side and I have to watch that he doesn't crash his arms into the doorway. Adjusting the height is a bit of a pain if you will be using it in different locations. If it is too high or low you have to take your child out and adjust it. Getting your child in and out is a little difficult put not impossible so that is why it is a pain to adjust.
My mother in-law bought this about 6months ago for my daughter who at the time was 5months.. She wasn't really into it when we first got it but the past 3-3and a half months she is completely in love with it. She goes crazy whenever my husband goes to pull it out of the closet to hang it up.. And I love the fact that you are able to adjust the straps. Since my daughter is on the short side, it was great she was still able to use it.. She tends to fall asleep in it while she bounces and plays with the music toys.. I do however have a little tiny issue with it..
It is kind of hard to get my daughter in and out of the seat without a little help.
I mean once your baby is able to move there hands and legs it gets little difficult.. But other then that it is wonderful.. I would defiantly recommend to all my family and friends!!
We had ordered this product a couple of weeks after we realized that our baby boy loved bouncing on our laps. so we could hardly wait for it to arrive. Once we got it and set it up, he quickly learned all the things he could do: play with the musical objects and jump to his heart's desire (or at least until Daddy or Mommy decided that it was naptime or time to change the diaper). We got the same noisy sound like other reviewers did. This comes from the spring inside the white tube that rubs against the inside of this white plastic tube. My Husband fixed it by putting some type of lubricant (not sure if it was WD40 or not). Also, it's a bit tougher than other activity toys to get our baby into and out of the seat. This is probably because of the way the supports are positioned. But this is a minor thing compared to the happiness it brings to our son and how it relieves me of having to hold him all the time, especially when a few weeks ago, he WAS still a supported sitter. Now he sits on his own! But this didn't hamper playtime in any way. He loves to jump up so high in it, especially when he gets excited such as when Mom and Dad are both watching him!


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Tools and techniques for creating custom Linux systems
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SUSE Joins the Microsoft Test Drive Program
Customers can take a free test drive of SLES for HPC on the Azure Cloud
SiFive Launches a Line of Open Source Chips
San Francisco-based chip company announces their first fully open source chip platform.
Fedora 24 Released
The whole distro gets rebuilt on glibc 2.3
Canonical Announces Cross-Distro Support for Snaps
Ubuntu Vendor tries to solve app packaging and distribution problem across distributions.
ownCloud Forked
Founder of ownCloud launches the Nextcloud project.
HP Enterprise Unveils a Futuristic Computer
Will The Machine change the way future programmers think about memory?
CoreOS Announces Distributed Storage System
The new Torus distributed storage system is available under an open source license on GitHub
Google Beats Oracle in the Android Lawsuit
Juries decides Google's use of Java APIs Was Fair Use
Linux is the Largest Software Development Project in History
But if you are not using the latest Linux kernel, your system is insecure.
Linksys Says Yes to Open Source Firmware
Home routers will give room for custom firmware but still comply with FCC rules


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I needed a hotel for an extended stay and found Candlewood to be the perfect location, clean rooms, and a very friendly and efficient staff. I would recommend this hotel to anyone.
- Official Description (provided by the hotel):
- Candlewood Suites Windsor Locks offers guests a comfortable extended stay. Conveniently situated near the Hartford Bradley International Airport, the Candlewood Suites Hotel in Windsor Locks, CT offers easy access to downtown Hartford, CT and Springfield, MA, as well as to top area attractions and businesses. Extended stay guests of our pet friendly Windsor Locks hotel can enjoy spending an afternoon at Six Flags New England or the Eastern States Exposition Grounds. Tours of the historic Mark Twain House, the New England Air Museum and the Springfield Basketball Hall of Fame are also less than 12 miles from our Windsor Locks, CT accommodations. While lodging in Windsor Locks, CT, business-oriented travelers can appreciate our hotel near Bradley International Airport and companies such as Hamilton Sundstrand, Ahlstrom, American Honda and Travelers. To best accommodate our guests' business needs, we offer over 900 sq. ft. of meeting space and a 24-hour business center. During your visit, you can enjoy the comforts of home and the convenience of a regular routine. A complimentary area shuttle and free high-speed Internet access are provided, and an indoor swimming pool, a well-equipped fitness center, a DVD lending library and an outdoor barbecue gazebo are also available on-site. As you plan your trip, we hope you will explore our exceptional Windsor Locks hotel rates and make your reservations with us today. ... more less
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