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My wife is reading 50 Shades of Gray. Your thoughts...
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can anyone give some advice i really need to hear some honest advice.
eve incognito 27803051
any kind of abuse, even if it is only slightly sexually tinted, will open unwanted doors in your psychology. it's ok to explore what's beyond those doors, but please make sure you stay on top of things (pun not intended).
Anonymous Coward 15782258
i appreciate the effort, and i wish you could elaborate your opionion a bit.
i don't think you understood properly, i'm pretty heatlhy sexually, the abuse never twisted anything much in me, maybe it got me a little bit more inhibited then i'd liek to be, but alos i was never really in love as much as i am now, so that could be it.
we wernt experimenting with SM, he thought i cheated on him to humiliate him in front of his friends, and he was funrious and he ahd furious, me tarzan u jane sex with me.
it was still scary, very disrepsceful and hurtful, and nother me nor my bf like id very much.
he enver did it before that or after, but i am afraid it will hapen again, enxt time he misunderstands me, and quite honestly it messed me up greatly more than any kind of abuse or hardship i ever experienced in my life.
the thing that makes me think im not well anymore is exactly the fact that i love him.
even after he id that i love him.
but also, given my history of abuse, maybe i am too se3nsitive to stuff.
maybe it wasnt as bad as i think it was.
he was wrong to do it i am not excusing his behaviour but maybe some woman who was never raped wld understand that her bf is a very passionate man, he was hurt and angry and he took things too far, but he didn't hit me, or humiliate me, he was just furious, acting macho and not considered of the fact that his huge penis will do internat damage if he keeps on thrusting that ahrd.
i was scared and naturally couldnt climax, but he dint like what he was doing so he cldnt to, whcih only prolonged my agony...
i remmebr myself saying would you please cum, i am starting to hurt, and i proceded to beg him to cum, not coz i was scyred he'd hurt me but coz i knew he had to be mad and obviously that made him not careful about his penis and frankly it was starting to hrt a bit, but i was determined to stick it out, coz he obviously had this ened to man handle me and get it out of his system
again, he wasnt abusive, he was jsut not very careful and i couldve stopped him but i never attempted to stop him i knew he msutve been feeling awful thinkkinh his own gf got her kicks out of humiliating him, so i wanted him to just have an orgasm, see that this will not make him feel better, or fix anything and realize we have to work things out in anone aggressive manner.
but he cldnt come or he dint want to do it before i did, and i cldnt coz i was scared, sdo it prolonged everything a bit and ended up a total catastrophy.
he dint rape me or anything, or abuse me, he just let his pride get the best of him.
i am not defending him but he is 27 years old, he was 26 at the time and he was manipulated by people older than him and less passionate but more calculated, into thinking that he isnt aplha enough with me and thats why i cheated.
ia m not defending him, but 26 year old people are still people of the heart, they did not learn yet that they have no freinds in this world and my bf took some very bad advice from a man who was supposetly his best friend, but in reality lied to him coz he wanted me to break up with my bf coz he wanted to sleep with me.
since then my bf learend he didnt have a freind in this man and made some new freinds with decent men...
do u still think i should leave him?
eve 27803051
Sounds like he has trust issues. Trust is pretty hard to gain especially when something in their past prevents them from trusting others( I remember when I found out one of my past exes cheated, it took a while to trust another woman again). As for the sex, thats pretty odd that he does that and you're actually putting up with it. Him seeing you put up with it means that he can do it as much as he wants. Have you tried telling him "hey this needs to stop" ?
I'd leave the guy, but you love him, so that puts you in a tough spot.
he doesn't do it. he did it once, under very very strange circumstances....
ur right about trust issues, only itps not him i had trust issues when i met him.
i was running away from very bad people, i had bee married prior to meeting my bf and the guy i was married to abused me.
so i was still very sensitive and in a wierd place when i met my bf....
this is exactly my dilemma here....
was i overreacting to what my bf did, coz i asceped from an abusive spouse and was afraid that my bf is exactly like my ex...
or did i just lose my faculties a bit after everything...
actually no i didnt lose my faculites, my faculties are all in place....
I JUST LOVE HIM; abdul, that' his name....
i cannot imagine what my life wld look like without him....
but what if we try again and next time someone is after me, coz i do not know if there are still people out there from the mafia that was after me....what happens next time my husband cant understand why im acting strange...
will he again procede to hurt me....
he was really nasty to me....he told me i was ugly, old
(and i am older than him, and in all honesty less atractive then him)
i was stunned and confused after the rough sex i said u raped me ??? coz i wasnt sure if he was attepmting to rape me or what the hell was he thinking..
he said ur ugly old why wld i rape u look at urself look at me....
it was really quite bad at the time....
now he says he dint mean it...that he doesnt say stuff he means when angry, but quite the opposite of what he really means....
but i was left with the feeling that it was exceptionally cruel of him, since he was younger and better looking to point that out in such a cruel manner....
i mean i know what i look like...and i see what he looks like....i know he is both younger and better looking....
i just thought it wasnt that important if people love each other....
but once ur younger better looking bf calls u and ugly bitch, u start wondering if he really thinks so but only says it when mad, or maybe he was jsut very hurt...
still...if i was better looking i cld understand him saying that knowing that i was pretty so it was ok to swing such cheap shots....but in reality i am not that pretty....
so he jsut pointed out the facts, that i am not that pretty....
i'd never call an ugly person ugly....
that is jsut low.....
sometimes if im angry i can call a pretty person ugly, coz nobody likes to be called ugly, but pretty people can get over it....
but he basically called his ugly gf ugly, now that is exceptionally cruel....
i have a mirror i know im not that attractive, but i make up for it with great personality and very alive spirit....still....
who calls an ugly person ugly, even if they are abgry...that is jsut too cruel for school....
any thoughts?
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|Publication number
|Publication type
|Application number
|Publication date
|Jul 3, 2012
|Priority date
|Dec 7, 2007
|Fee status
|Also published as
|Publication number
|Thomas Downing
|Original Assignee
|Thomas Downing
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (20), Referenced by (2), Classifications (23), Legal Events (1)
|External Links: USPTO, USPTO Assignment, Espacenet
There is no cross-reference to related applications.
The present invention generally relates to polymer synthesis. More particularly, the present invention is an improved apparatus which is highly efficient.
To meet the increasing demand for nucleic acid synthesis, there has been an increase in the variety of designs, and the volume of production of nucleic acid synthesizers. Unfortunately, the currently available synthesizers are not designed to adequately meet the needs of the industry. In particular, available synthesizers are limited in their ability to efficiently synthesize large numbers of oligonucleotides. While synthesizers have been developed to simultaneously synthesize more than one oligonucleotide at a time, such machines are not efficient at the production of different types of nucleic acids simultaneously (e.g., different lengths of nucleic acids) and are unacceptably prone to performance failures and environmental contamination. Furthermore, available synthesizers are not suitably configured for integration into large-scale automated production facilities.
DNA synthesis is presently performed on automated instruments which are capable of concurrently producing multiple DNA segments. Frequently the apparatus uses reaction columns in which a support material for the reaction is positioned within the columns on top of inert, porous filters, referred to as frits. The support material generally has a starter material bound to the support onto which desired oligonucleotides may be synthesized. The reaction columns are placed within the automated apparatus and chemicals are added to the columns in sequence in appropriate amounts in an automated fashion. In order to address today's large demand for high throughput oligosynthesis, most automated apparatuses have a large footprint and take up a great deal of premium laboratory space.
Most currently known automated synthesizers can produce only a few oligonucleotides at a time, which is limited by the number of reaction columns located within the machines. The number of reaction columns is limited as a practical matter by the increased complexity of the plumbing and valving network, as the number of columns increases. In addition, the system must be airtight to avoid contaminating the chemicals with air or water and to avoid human exposure to the chemicals.
U.S. Pat. No. 5,368,823 issued Nov. 29, 1994, and U.S. Pat. No. 5,541,314 issued Jul. 30, 1996, address the need for producing a large number of oligonucleotides by disclosing a method and apparatus for oligonucleotide synthesis in which the plumbing and valving network is simplified. The patents disclose a system in which there is one supply line and one outlet located in the synthesis chamber for the delivery of reagents into the reaction columns. The outlet can be positioned above the inlet end of each of the columns so that nucleotide reagents, capping reagents, deblocking reagents, wash chemicals, etc. can be provided to each of the reaction columns. All of the reagents are located in a supply system which includes reservoirs and valving to connect the reservoirs with the supply line. A flush/prime column is also located within the chamber so that the supply line can be flushed and primed between each different chemical reagent addition. A vacuum source, located outside of the reaction chamber, is connected to the outlet end of the reaction columns to rapidly draw the chemicals from all columns simultaneously, thus leaving the columns dry and ready to receive the next reagent.
The disclosed apparatus in these two patents provides multiple reaction columns, but the single supply line requires flushing and priming between the addition of each reagent. These steps are time consuming and waste reagents. Moreover, a large footprint is required to accommodate a reaction chamber encompassing the moving supply line and the reaction chambers as well as a vacuum source outside of the reaction chamber. The large footprint is a drawback to space-constrained laboratories.
Another group of patents, U.S. Pat. No. 5,472,672 issued Dec. 5, 1995, U.S. Pat. No. 5,529,756 issued Jun. 25, 1996, and U.S. Pat. No. 5,837,858 issued Nov. 17, 1998, addresses the need for high throughput oligosynthesis by disclosing a polymer synthesis apparatus with many stationary supply lines. The patents disclose an apparatus with a head assembly with many nozzles, with each nozzle coupled to a reagent reservoir. Further, a base assembly has at least one reaction well but can utilize 96-well and other plates. A transport mechanism is coupled to the head assembly and/or base assembly to produce relative movement between the two. The transport mechanism moves horizontally to align a selected reaction well and a selected nozzle to deposit a selected liquid reagent into the reaction well for synthesis of a polymer chain. A sliding seal is positioned between the head assembly and the base assembly to form a common chamber that encloses both the reaction wells and nozzles therein. The seal is constantly being rubbed down by the movement of the metal piece back and forth to move the synthesis block. This wearing down of the seal results in a less efficient seal.
Thus, the art is in need of polymer synthesizers that are efficient, flexible, and are amenable to large-scale production and automation for the synthesis of polymer, and more specifically of DNA.
It is a first object of the present invention to provide an apparatus to synthesize polymers having high output efficiency.
Another object of the present invention is to provide an apparatus to synthesize polymers which needs less inert gas during the synthesis.
A further object of the present invention is to provide an apparatus to synthesize polymers which is capable of producing a plurality of polymers in the same batch.
Other and further objects and advantages of the present invention will be obvious upon an understanding of the illustrative embodiments about to be described or will be indicated in the appended claims, and various advantages not referred to herein will occur to one skilled in the art upon employment of the invention in practice.
A polymer synthesizer having an airtight cabinet configured to maintain a positive gas pressure, comprising a lid, synthesis columns having a lower extremity, and connected to a plurality of reagent containers through reagent dispense lines and wherein the reagents are dispensed with tip dispensers into the synthesis columns, comprising a block comprising a plurality of waste columns disposed in groups, each of the waste column being adapted to receive the lower extremity of one of the synthesis column; a plurality of waste channels, wherein each waste column of one of the groups is continuously connected to a single waste channel, and wherein all of the groups are simultaneously connected to their corresponding waste channels; and a plurality of waste channel valves connected to each of the waste channels to selectively drain at least one of the waste channels.
The polymer synthesizer may be used to synthesize polymers with a flow-through process, such as, for example, oligonucleotides, peptide nucleic acids (PNA), polypeptides, nucleic acids, DNA or RNA. The containers may also contain the solutions used in the reactions, such as deblocking agent, washing solution, coupling activator, capping agent, oxidizer or other solutions required for a reaction, which are also designated by the term "reagent" in the present document.
The synthesis columns are sealingly connected into the waste columns of the block to make an airtight fit with sealing means such as, for example, pressure fit, a flat mat or an o-ring. However, any other suitable sealing means may be used for this purpose. The synthesis of a polymer occurs in the synthesis column in a derivatized Controlled Porosity Glass (further referred as CPG), which is a solid support for covalent attachment of biological molecule, or parent molecule, as known in the art. The solid support can be derivatized CPG, polystyrene or any other convenient material having a large surface area and capable of being derivitized (during the process of derivitization, a protected nucleotide or linker is attached to which the growing DNA chain is attached). The CPG is a white powder held between two porous plastic frits. Other solid phase supports may also be held between frits or imbedded in a solid frit. The reagents are dispensed at the top of the synthesis column and left for an appropriate time, and then drained through waste column by overpressure. The sealed box in which all of this occurs is continuously pressurized. When a valve is opened to provide an exit pathway then the reagents on the columns will flow out the exit pathway provided. The next step of the synthesis is then carried out as others reagents are dispensed into the column until the polymer is completed. The reagents are dispensed into the synthesis columns by tip dispensers, which are controlled by valves, connected to the corresponding pressurized reagent containers. The number of tip dispensers installed on an apparatus is variable and adapted to the occurring synthesis. Furthermore, it is possible to synthesize different polymers in the different synthesis columns in the same batch or the same block. To further improve the efficiency of the apparatus, when multiple reagents are dispensed sequentially into the same synthesis columns without drainage, the less reactive reagent may be dispensed first into the corresponding synthesis column to save time and then the other reagents are dispensed. Usually the reagent containers (and order of dispensing) are alphabetically ordered with the name of the reagent but in the present invention, the reagent containers are decreasingly ordered depending of the reagent reactivity increasing again the efficiency of the apparatus. To further improve efficiency and to avoid errors, the synthesis columns may be optically identified and verified by their color when they are color coded, prior to or at the beginning of a synthesis run, as known in prior art.
The airtight cabinet has a lid on the top to place or remove the block and the synthesis columns. The tip dispensers are sealingly fixed in the lid and a translucent window may be installed in the lid. They may be threaded to the lid or inserted in a hole and sealed with sealing means. The lid configuration may be interchangeable to allow a different number or configuration of the tip dispensers.
To displace the block, two linear slides, controlled by a servomotor or step motor, are used in both the y and x axes in a horizontal plane. The block may be adapted to be disposed directly on the slides or on a movable plate connected on the slides and adapted to receive a block. This block is moved so as to place the appropriate synthesis column under one of the dispense tips so that a specific reagent can be dispensed into that synthesis column. An optical device may be used to identify a synthesis column prior to the displacement. A plurality of synthesis column may be filled by a plurality of tip dispensers at the same time with different reagents. The motion of the slides is controlled by a computer which sends the appropriate instructions to the valves and motion controllers of the slides. The computer can be either on-board or outside the apparatus. Several polymer synthesizers can be controlled by one computer.
The block is used to hold the synthesis columns by their lower extremity and to drain the waste, or used reagents, to a waste container. The block is a structure having a two dimensional matrix of vertical holes, or waste columns, that are connected to a waste container through waste channels. The waste channels may drain the waste of a plurality of synthesis columns or only one synthesis column. The evacuation of a plurality of waste columns or a sole waste column is controlled by a valve installed on the waste line that is preferably controlled by a computer. The reagent is allowed to stay in the synthesis column for a defined amount of time and is drained through the synthesis column to waste with overpressure when the waste valve is opened. Because a plurality of waste columns or a sole column may be controlled independently from the others, there is no need to wait for other longer reactions to drain wastes, increasing the efficiency of the apparatus. Each group of columns which is continuously connected to a waste channel functions independently of the other groups or banks. Each group may therefore use an entirely different synthesis cycle. A purge waste port is also provided to drain and/or to prime a dispense lines.
The block may be composed by only one part or by a plurality or parts. For example, the block may be composed by two parts; a spacer block, or top part, and an o-ring block or bottom part. The idea is to fix the sealing means, in this case o-rings, in the waste columns of the bottom part. When the synthesis columns are inserted in the two part block, the pressure tight seal is made by the pressure of the o-ring on the column. This allows for a more effective seal since the columns may have a slightly different size. In addition, the pressure required to insert or remove the columns is much less than in a design with a pressure fit to fix the synthesis column into the waste columns. In this example the sealing means are inserted into the bottom part but they may be inserted at the bottom extremity of the spacer or any other suitable positions.
A support may be used to hold a plurality of synthesis columns. The support allows the columns to be inserted all at once and is then inserted and removed as a unit into the block. Using a dimensionally slightly modified version of this same block, multiwell plates or synthesis plates may also be used in which the wells comprise an opening at their lower extremity and acts as synthesis columns.
The present invention provides also a method to synthesize one or more polymer by simultaneously chemically linking a plurality of monomers to a plurality of parent molecules. The method allow the making at least one type of polymers by chemically linking a plurality of monomers to a parent molecule attached in a synthesis column one at a time in an apparatus comprising an airtight cabinet with a lid, a block having a plurality of waste columns disposed on a horizontal plane having x and y axles, a plurality of synthesis columns connected to waste columns of said block by their lower extremity, and reagents being delivered from a plurality of reagent containers through reagent dispense lines and wherein the reagents are dispensed with tip dispensers into synthesis columns, the waste columns being controlled by waste valves, the method comprises displacing the block along both y axis and x axis to dispense reagents in synthesis columns through tip dispensers with means to displace said block, draining the synthesis columns by the opening of waste valves, repeating the dispensing of reagents and the draining of synthesis columns as needed for a polymer.
The chemical linkage may be, for example, a phosphodiester bond, a phosphorothioate bond, a phosphonate bond, a phosphoramidate bond, an amide bond, an imine bond, a carbamate bond, an azo bond, a sulfone bond, a sulfonide bond, a sulfonamide bond, a sulfide bond, a disulfide bond, an ether bond, an ester bond, a thiourea bond, a urea bond or a carbon-carbon bond.
The polymers synthesized by the present polymer synthesizer may be nucleic acids, DNA, RNA, peptide nucleic acids (PNA), polypeptides or any other product suitable for preparation by solid phase synthesis. The monomer may be a modified nucleotide comprising a minor groove binder.
In a preferred embodiment, the steps to create a molecule by chemical linkage include:
It is to be noted that the previous sequence is for only one monomer added. Any of the operations of this sequence may be repeated when necessary depending of the resulting polymers to be synthesized.
The features of the present invention, which are believed to be novel, are set forth with particularity in the appended claims.
The above and other objects, features and advantages of the invention will become more readily apparent from the following description, reference being made to the accompanying drawings in which:
A novel polymer synthesizer will be described hereinafter. Although the invention is described in terms of specific illustrative embodiment(s), it is to be understood that the embodiment(s) described herein are by way of example only and that the scope of the invention is not intended to be limited thereby.
The synthesis occurs in the synthesis column 140 which are connected to the block 110. The reagents are carried to the synthesis column 140 through the tip dispensers 162. The block 110 comprises the waste columns (not visible in
The wastes are drained with a plurality of lines of waste channels forming the waste system shown in
The polymer synthesizer is controlled by a control system as shown in
A first embodiment for the block 410 is shown in
The motion of the plate 520 is shown in
While illustrative and presently preferred embodiment(s) of the invention have been described in detail hereinabove, it is to be understood that the inventive concepts may be otherwise variously embodied and employed and that the appended claims are intended to be construed to include such variations except insofar as limited by the prior art.
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|University Of Georgia Research Foundation, Inc.
|Method for automated synthesis of oligonucleotides
|Dec 8, 1995
|Aug 26, 1997
|Moritex Corporation
|Automatic solid phase extraction device with interchangeable nozzle holder head
|Jul 20, 1995
|Oct 28, 1997
|Neves; Richard S.
|High throughput oligonucleotide synthesizer
|May 30, 1995
|Nov 17, 1998
|The Board Of Trustees Of The Leland Stanford Junior University
|Method for polymer synthesis using arrays
|May 28, 1999
|Oct 17, 2000
|Mettler-Toledo Bohdan, Inc.
|Pressurized reaction block
|May 4, 1999
|May 28, 2002
|Orchid Biosciences, Inc.
|Multiple fluid sample processor with single well addressability
|Mar 27, 2001
|Dec 9, 2003
|Irm, Llc
|Apparatus and method for preparing fluid mixtures
|Dec 13, 2000
|Dec 16, 2003
|Illumina, Inc.
|Oligonucleotide synthesizer
|Nov 28, 2000
|Sep 7, 2004
|Symyx Technologies, Inc.
|Parallel reactor with internal sensing and method of using same
|Mar 16, 2000
|Oct 5, 2004
|Scott P. Hunicke-Smith
|Polymer synthesizer
|Mar 8, 2001
|Mar 22, 2005
|Tibotec Bvba
|Method and apparatus for dispensing a liquid into a series of wells
|Jan 26, 2001
|Aug 23, 2005
|Third Wave Technologies
|Nucleic acid synthesizers
|Apr 11, 2003
|Apr 11, 2006
|Illumina, Inc.
|Apparatus and methods for reformatting liquid samples
|Nov 14, 2001
|Apr 25, 2006
|Shafer David A
|Expression miniarrays and uses thereof
|Oct 29, 2001
|Jun 6, 2006
|Tecan Trading Ag
|Device and system for dispensing or aspirating/dispensing liquid samples
|Feb 22, 2001
|Mar 7, 2002
|Jianming Xiao
|Method and apparatus based on bundled capillaries for high throughput screening
|Aug 15, 2001
|Jun 19, 2003
|Third Wave Technologies, Inc.
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|Aug 15, 2001
|Jul 3, 2003
|Third Wave Technologies, Inc.
|Polymer synthesizer
|Jan 5, 1999
|Jul 15, 1999
|Cartesian Technologies, Inc.
|Method and apparatus for high-speed dot array dispensing
|Citing Patent
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|Publication date
|Feb 22, 2011
|Nov 12, 2013
|Oligoco, Inc.
|Wicking-based reagent-solution draining in an automated system
|Sep 29, 2011
|Hsing-Yeh Parker
|Wicking-based reagent-solution draining in an automated system
|U.S. Classification
|International Classification
|Cooperative Classification
|European Classification


text:
Top Definition
to drive around aimlessly while smoking marijuana usually with other people, but can be done alone
There's nothing going on tonight, so we're just gonna route all night.
od uživatele Stevie P 05. červen 2006
Trending Now
To leave quickly, or to run away.
When the cops raided the keg everyone routed.
od uživatele brian. 07. listopad 2007
To leave a place. A double entendre combining a shortening of the phrase "roll out" and the noun "route".
Yo this party's a sausage fest, tryin' to route?
od uživatele Quizzle Rat 04. říjen 2007
A female who is a hoe..
Someone who sleeps around usually a girl
Someone who sleeps around usually a girl
Yo, I'm bout to go over this routes house so I could hit.
od uživatele Tr3y 22. duben 2007


text:
Last update: Tuesday 19 July 2016 [664 news items]
Taiwan market: Samsung unveils entry-level tablet Galaxy Tab A
Apr 28, 11:41
Samsung Electronics has unveiled Galaxy Tab A, an entry-level tablet with an S Pen touch pen, for launch in the Taiwan market in early May.
AMD to launch Godavari APUs at end of May, say Taiwan makers
Apr 27, 11:04
AMD will launch Godavari series APUs at the end of May to compete with Intel's Broadwell and Skylake platforms, according to Taiwan-based supply chain makers.
Samsung tops global smart TV market in 2014
Apr 13, 22:00
Samsung Electronics held a 28.2% share in the global smart TV market during 2014, the largest stake compared to any other vendor, while LG Electronics held a 15.2% share, according...
Taiwan market: HTC ranks as top smartphone vendor in 2014, says IDC
Apr 8, 22:06
HTC ranked as the number one smartphone vendor in the Taiwan market in 2014, followed by Samsung Electronics, Asustek Computer, Apple and Sony Mobile Communications, according to...
Digitimes Research: Samsung expected to see increasing orders on 14nm process
Apr 2, 12:01
Qualcomm's next-generation application processor (AP), the Snapdragon 820, is expected to outsource to Samsung Electronics using a 14nm node, which the Korea-based foundry house has...
Samsung reportedly ranks among highest average employee salaries
Apr 1, 22:05
Korea media reports have stated the average yearly salary at Samsung Electronics broke the US$90,000 mark in 2014 to rank among the top average-paid companies in South Korea.
Taiwan market: Samsung launches Galaxy S6, S6 Edge
Mar 27, 13:12
Samsung Electronics has unveiled its new flagship family products, the Galaxy S6 and Galaxy 6S Edge, in the Taiwan market and the new smartphones will be available starting April...
Samsung loses top position in global 4Q14 smartphone market, says Gartner
Mar 4, 22:11
Samsung lost the top spot to Apple in the global smartphone market in the fourth quarter of 2014, according to Gartner. Samsung had been number one since 2012.
Samsung, LG and Sony to purchase 42% of global TV panel supply in 2015, says IHS
Mar 2, 21:28
Leading global TV vendors Samsung Electronics, LG Electronics and Sony, gained market share and increased their on-year shares of LCD TV shipments by an average of 11% in 2014, which...
Samsung to adopt 20nm process for over 50% of its DRAM output in 2015, say sources
Feb 17, 11:01
Samsung Electronics began mass production of DRAM memory using 20nm process technology in 2014. The node technology will be adopted by the vendor for more than 50% of its overall...
Global 4Q14 DRAM output value rises 8%, says DRAMeXchange
Feb 11, 15:31
Worldwide DRAM output came to US$12.98 billion in value during the fourth quarter of 2014, up 8.2% sequentially, according to DRAMeXchange. Samsung Electronics continued to lead the...
Global NAND flash market rises 2% in 4Q14, says DRAMeXchange
Feb 10, 20:11
The worldwide NAND flash memory market posted revenues of US$8.75 billion in the fourth quarter of 2014, up 2% sequentially, according to DRAMeXchange. Samsung Electronics remained...
Samsung mass producing high-density ePoP memory for Smartphones
Feb 4, 20:52
Samsung Electronics has announced that the company is mass producing ePoP (embedded package on package) memory, a single memory package consisting of 3GB LPDDR3 DRAM, 32GB eMMC and...
Taiwan market: Samsung cuts 55-inch Ultra HD TV pricing below US$1,000
Feb 4, 20:49
Samsung Electronics is holding sales events in Taiwan to promote Ultra HD TVs, with 55-inch units now being sold for just under NT$30,000 (US$1,000), according to local retailers.
Samsung to increase smartphone production in India, says paper
Jan 29, 14:38
Samsung Electronics, in view of fast growing demand for smartphones in India, will invest INR5.17 billion (US$84.23 million) in its smartphone factory there to expand production,...
Samsung reportedly to expand DRAM capacity
Jan 28, 10:52
Samsung will build additional DRAM production capacity during 2015, which may create uncertainty for the industry bit supply growth and pricing, according to industry sources.
Samsung starts smartphone production lines in Indonesia, says report
Jan 27, 15:07
Samsung Electronics' production lines for smartphones in Indonesia have reportedly started operating recently, according to a Korean-language Edaily report citing sources...
Samsung and Globalfoundries reportedly win majority of A9 orders
Jan 15, 23:17
Samsung Electronics and Globalfoundries have secured a combined 70% of orders for Apple's A9 processors with their 14nm FinFET technology, according to industry sources.
Samsung said to dump Qualcomm chip in Galaxy S6
Jan 14, 23:10
Rumors have circulated in the industry that Samsung Electronics will dump Qualcomm's Snapdragon 810 processor for use in its next-generation Galaxy S-series flagship smartphone, in...
Samsung reportedly grabs processor orders for Apple Watch
Jan 9, 10:37
Samsung Electronics' 28nm process technology has reportedly obtained orders for the Apple Watch set for launch in March 2015. The orders call for 3,000-4,000 12-inch wafers monthly,...
Samsung to post lower sales and profits for 2014
Jan 8, 21:21
Based on the company's guidance for the fourth quarter, Samsung Electronics is set to post a sales decline in 2014.
Sony ready to launch large-size tablet in 1H15
Dec 25, 14:57
In addition to Samsung Electronics and Apple, Sony is also expected to launch a large-size tablet in the first half of 2015 and the device will enter mass production shortly, according...
Taiwan market: Samsung launches MILC Smart Camera NX1
Dec 8, 21:32
Samsung Electronics on December 8 unveiled its Smart Camera NX1, a flagship MILC (mirrorless interchangeable-lens camera), for immediate launch in the Taiwan market at a recommended...
Samsung offers large price cuts for Ultra HD TVs in North America
Dec 4, 15:40
Samsung Electronics has made large reductions in the retail prices of Ultra HD LCD TVs in the North America market. Prices at Best Buy have been cut by US$700 from US$1,499 to US$799...
Asustek expects to achieve 2015 shipment goals despite pessimistic outlook
Dec 4, 15:29
Despite many research firms having estimated that global tablet and smartphone shipment growths will dramatically weaken in 2015 and Apple, Samsung Electronics and Asustek Computer...
Samsung Electro-Mechanics to expand businesses in China in 2015, says paper
Nov 27, 16:05
The Samsung Group's component manufacturing affiliate Samsung Electro-Mechanics has seen its performance seriously impacted by Samsung Electronics' weakening smartphone sales recently...
Mobile DRAM revenues rise 6% in 3Q14, says DRAMeXchange
Nov 27, 12:24
Revenues for mobile DRAM reached US$3.46 billion in the third quarter of 2014, up 6% from US$2.27 billion in the second quarter, according to DRAMeXchange. Growth was driven by increased...
BOE shifts focus from Samsung to China vendors
Nov 26, 14:51
China-based panel maker BOE is shifting its focus away from Samsung Electronics to China-based vendors as Samsung's smartphone sales have recently seen lower-than-expected results.
TSMC to grab 40-50% of A9 chip orders
Nov 26, 13:56
Taiwan Semiconductor Manufacturing Company (TSMC) is expected to secure a combined 40-50% of Apple's A9 processor orders for next-generation iPad and iPhone devices, according to...
Supply chains gearing up for expected flexible display demand in 2015
Nov 19, 15:12
Following developments by Samsung Electronics and LG Electronics to produce devices with flexible displays, panel makers from China, Taiwan and Japan, and related component supply...
Samsung, TSMC still competing for Apple A9 chip orders
Nov 4, 00:23
Samsung Electronics and Taiwan Semiconductor Manufacturing Company (TSMC) are still in the race for orders for next-generation SoCs from Apple, which is expected to confirm the chip...
Samsung to quit LED lighting business in overseas markets, says paper
Oct 28, 14:20
Samsung Electronics on October 26 announced the stoppage of its LED lighting business operations in all overseas markets due to low-price competition, but it will continue production...
Dell, Asustek and Lenovo eye Chromebook market
Oct 28, 13:59
Seeing that Chromebooks are enjoying demand from the education sector, brand vendors such as Dell, Asustek Computer and Lenovo have started becoming aggressive about the market, while...
Taiwan market: HTC maintains largest market share by sales volume in September
Newswatch - Oct 24, 09:45
There were 730,000 handsets sold in the Taiwan market in September and HTC maintained the largest market share at 20.8% in terms of sales volume, followed by Samsung Electronics with...
Samsung, TSMC Solid State Lighting reportedly to quit LED lighting business, say Taiwan makers
Oct 21, 22:23
Samsung Electronics will reportedly withdraw from the global LED lighting market except South Korea due to low-price pressure from China-based makers, while Taiwan-based TSMC Solid...
Coretronic nets NT$1.13 per share for 1H16
Displays | 1h 58min ago
MLS upbeat about synergy with Osram
LED | 3h 5min ago
AUO to start 6G LTPS production in eastern China in 4Q16
Displays | 3h 14min ago
Nanya, Inotera deals with Micron still proceeding
Bits + chips | 3h 26min ago
Nuvoton reports NT$1.25 EPS for 2Q16
Bits + chips | 3h 28min ago
Analog Devices, Linear to merge
Bits + chips | Jul 27, 21:35
UMC looks to 2-3% shipment growth in 3Q16
Bits + chips | Jul 27, 21:34
BlackBerry launches new Android smartphone
Mobile + telecom - Newswatch | Jul 27, 21:33
SPIL reports NT$0.90 EPS for 2Q16
Bits + chips | Jul 27, 21:32
Pegatron automates factory in Shanghai, says chairman
IT + CE | Jul 27, 21:31
Nanya 2Q16 profits fall
Bits + chips | Jul 27, 21:28
Connector maker Sinbon reports earnings for 2Q16
IT + CE - Newswatch | Jul 27, 21:26
Digitimes Research: Taiwan ships nearly 8.5 million LCD TVs in 2Q16
Displays | Jul 27, 21:26
Digitimes Research: Over 4,000 models of LTE-enabled devices launched as of February 2016
Mobile + telecom | Jul 27, 21:24
Innolux turns to focus on shipping whole-set products to increase profits
Before Going to Press | Jul 27, 22:00
Sharp reportedly sees improving profitability
Before Going to Press | Jul 27, 21:59
Xiaomi announces new Mi Book Air notebooks
Before Going to Press | Jul 27, 21:11
Connector maker Sinbon reports earnings for 2Q16
Before Going to Press | Jul 27, 21:10
Taiwan sets aside budget of NT$107.85 billion for development of science and technology in 2017
Before Going to Press | Jul 27, 21:10
China reaches total PV installation capacity of 22GWp in 1H16, says NEA
Before Going to Press | Jul 27, 21:09
LeEco to acquire Vizio
Before Going to Press | Jul 27, 21:08
BlackBerry launches new Android smartphone
Before Going to Press | Jul 27, 21:07
Vizio acquisition expected to push LeTV-Foxconn partnership
Before Going to Press | Jul 27, 21:06
- Osram to sell lamp business unit to China-based consortium
- AimCore 2Q16 operations may turn profitable
- AUO reports operating profit for 2Q16
- Notebook players less keen to adopt USB Type C
- Samsung likely to unveil Galaxy Note 7 on August 2
- Taiwan to be flexible in allocating frequency band for IoT, says minister
- ET Solar, LONGi set up joint venture for PV module marketing
- State Power Investment procures 2.75GWp of PV modules
- Component makers to see weak orders for tablets and wearables
- China may lower target PV installation capacity in 13th 5-year plan
- HTC to face increasing competition in VR market in 2H16
- E-sports expected to push demand for gaming notebooks, desktops in 2H16
- Hangzhou factories concerned about impact of air pollution controls during G20 Summit
- Passive component maker Prosperity Dielectrics reports increased earnings for 1H16
- Digitimes Research: Launch of Android-based Pepper to ramp up market size
- Foxlink developing service-oriented robot products
- Young Optics suffers 1H16 net loss per share of NT$1.26
- Chip orders for notebooks from Huawei and Xiaomi falling, say sources
- OLED to become leading smartphone display technology in 2020, IHS says
- PTI posts profit growth in 2Q16
- Realtek 2Q16 profits soar over 70%
- Digitimes Research: Taiwan ships over 269 million small- to medium-size panels in 2Q16
- POS device supplier Posiflex reports strong earnings for 1H16
- Intel Dalian fab put into operation, says report
- AMD introduces new professional graphics
- Releases
- White papers
- Bulletin
- EuroBLECH 2016 reflects trend towards smart manufacturing in sheet metal working
- Advantest rated as Advanced Masks Technology Center GmbH & Co. KG's (AMTC) best supplier 2015 for the second year in a row
- CAYIN to rollout system software upgrade and new product line
- Advantest introduces V93000 wave scale cards for cost-effective testing of current and next-generation RF semiconductors
- Computer-on-modules deliver an ideal solution for Industry 4.0 intelligent automation
- Unisys IT Implementation of Unisys Stealth Solution for Data Center Segmentation
- AccuTouch Five Wire Resistive Touchscreens
- Getting the Most Out Of Your Next Generation Digital Security Surveillance System
- Embracing the New Generation Intel Atom Family with DDR3 Memory Support
- Got The Message? Ensuring efficient and reliable delivery of content across a mission critical digital signage network
- Perforce Software Wins Develop Industry Excellence Award for the Second Year in a Row
- Perforce Teams with Leading Git Client Developer fournova
- Perforce Introduces Helix GitSwarm: Flexible Git Ecosystem Mirrored to a Powerful Mainline Repository
- Perforce Helix Distributed Version Control System Brings Greater Flexibility While Maintaining Enterprise-class Security, Auditability and Control
- Perforce Powers Development of Legacy Quest for iOS and Android
28-Jul-2016 markets closed
|TSE electronic
|OTC electronic
- Verizon to end Yahoo survival fight with US$4.8 billion deal (Jul 25) - Bloomberg
- China sees slowest growth since financial crisis (Jul 14) - CNNMoney
- Messaging app Line sees shares surge 50% in Tokyo debut (Jul 15) - BBC News
- Major win for Microsoft in 'free for all' data case (Jul 15) - BBC News
- IBM rolls out job-cutting plan in the Netherlands (Jul 7) - EE Times
- Typhoon Nepartak: Taiwan on high alert as storm approaches (Jul 7) - BBC News
- In race to be Asia's fintech hub, Singapore leads Hong Kong (Jul 3) - Reuters
- BMW said to team with Intel, Mobileye on self-driving cars (Jun 30) - Bloomberg
- Digitimes Research: 4 million 9.7-inch iPad Pros to ship in 1H16
- Digitimes Research: Global all-in-one PC shipments to drop 3.9% on year in 2015
- Mentor Graphics Veloce VirtuaLAB adds protocols for networking designs
- Mouser Electronics undergoing expansion
- Digitimes Research: iPad Pro shipments to be less than 3 million units in 4Q15
Industry integration, 5G and IoT applications: Q&A with Qualcomm president Derek Aberle
Qualcomm president Derek Aberle spared his time with Digitimes for an exclusive interview...
The gaming notebook competition: Q&A with Jone Chang from Gigabyte notebook department
Gaming notebooks have become the main focus of Gigabyte Technology's notebook department and despite...
Powering the cloud: Intel embraces new identity
The next wave of computing is now beginning to define the IT industry, where the boundaries of computing...
A new cycle begins for desktop motherboards: Q&A with Gigabyte technical marketing manager, Leon Chen
The launch of virtual reality headsets for the consumer market and the increasing demand for ultra...
- Smartphone and tablet industry: The view from Taiwan - May 2012
Taiwan-based companies have a long and successful history of involvement in the supply chain for mobile devices, which for the purposes of this article consist...
- Taiwan motherboard industry overview - May 2012
The year 2011 saw the motherboard industry reverse its trend of declining shipments year-on-year, to post a slight 0.2% growth. This upturn in fortunes was expected...
- Notebook market overview - Jun 2011
The notebook industry in 2010 experienced several major events that significantly changed the industry's ecosystem for the year and the industry is also believed...
- LCD TV overview - Jun 2011
Growth of the global LCD TV market was not as strong as expected in 2010 as the market continued to feel the aftershocks of the economic recession that had hit...
- Wireless broadband developments in Southeast Asia markets
As of 2013, the 10 ASEAN nations had a total of over 700 million mobile subscriptions, with the CAGR from 2003-2013 reaching 24%. This Digitimes Research Special Report analyzes the various mobile broadband markets in ASEAN and looks at the respective trends in 4G LTE development for those markets.
- 2015 global tablet demand forecast
This Digitimes Research Special Report provides a 2015 forecast for the global tablet market and analyzes the strategies of key market players such as Google, Apple, Intel, and Microsoft.
- 2015 China smartphone panel trend forecast
This Digitimes Research Special Report analyzes the strategies of key China-based major panel makers BOE, Tianma and IVO for attacking the different market segments through technology and pricing, and their relationship to local vendors Huawei, Lenovo, ZTE, Xiaomi and Coolpad.


text:
- Miss Florida 2017 stripped of crown days after win - US Weekly
- Lindsay Lohan steps out amid relationship drama - TMZ
- Was Taylor Swift snubbed by the VMAs? - Gossip Cop
- Rachel Platten sings "Fight Song" with lots of stars - Just Jared Jr
- Farrah Abraham sizzles in a revealing bikini - Radar
- New pics from the Clooneys' Italian summer - Lainey Gossip
- Fox News snubs major DNC moment - The Hollywood Reporter
13 December 2012 at 1:56 AM
Megan Fox: 'This Is 40' Premiere with Paul Rudd!
About this photo set: Megan Fox walks the red carpet at the premiere of her new film This Is 40 on Wednesday (December 12) at Grauman's Chinese Theatre in Hollywood. The 26-year-old… Read More Here
Photo #: 2774980
Posted to Megan Fox, Paul Rudd, This Is 40
powered by ZergNet


text:
We Need You
Volunteers just like you are an integral part of all we do at Awbury. When you volunteer, you make a difference for your entire community to connect with nature and history. Thank you for taking your time to invest in others and their future!
Whether you love to work outside in the garden, enjoy hosting events, like to lead tours, or prefer office tasks – we'd love to have you as part of our team!
Awbury welcomes volunteer groups. Join us to help with spring and fall plantings, invasive species removal, trail maintenance, pruning, mulching, litter patrol, and seed collection!
Fill out the form at the bottom of the page to let us know what you're interested in!
Opportunities for Awbury Volunteers:
Pollinator Habitat 2016 Seasonal Open Gardening Hours
Tuesday afternoons, Thursday mornings, and the last Sunday of each month*
Gardening to benefit pollinators is a wonderful focus to add to your gardening skills repertoire. Awbury's Pollinator Habitat is located in Awbury's Agricultural Village and is maintained by Penn State Extension Master Gardeners, but all gardeners are welcome to join! Come learn, share, and enjoy our pollinator-friendly garden.
* Start times may vary slightly. RSVP for schedule information and updates.
Led by Master Gardeners Leslie Cerf and Marty Hudson
Second Saturday Volunteer Days
Saturdays from June – October, 9:00 AM – 1:00 PM
Two shifts of gardening in the historic English Landscape:
– Followed by a tour of the Arboretum
Meet at the Cope House parking lot.
Led by Volunteer Coordinator Leslie Cerf
Volunteer at our
Heartwood Music Festival!
Sunday, June 12, 9:00 AM – 7:00 PM
Help us celebrate our centennial year at this day-long music festival, plus circus performances, petting zoo, disc golf course, kids' crafts, face painting, and much more.
We have a variety of roles and shifts for volunteers to fill, including staff support and children's activities.


text:
Our informational parent meeting will be 2/23/16. This year's team will be 5th grade & up. If you are interested in joining the team, contact Kelly Hutt at firstname.lastname@example.com. First practice will be Monday, Feb. 29th at 6PM @SCHS track. Please bring a copy of a current physical & $25 athletic fee to begin participating.
Spencer County Middle School
40071, Taylorsville, KY 40071
Phone: 502-594-4252
Home | Roster | Alumni | Schedule | Season Bests | Relay | Records | Announcements | Articles | Fan Shop
Team Announcements
We will have xc conditioning from July 6th thru July 9th and July 13th thru July 16th from 6-7:30 at the overlook at Taylorsville lake. The following week (July 16th thru July 19th) will be tryouts, any question please contact me at 594-4252.
GuestbookPost a comment
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text:
Theatre Ancaster
Theatre Ancaster is a community musical theatre company with a reputation for quality productions. Our mandate is to present excellent quality live musical theatre to the community and to assist in the development of musical theatre among the adults and youth of the community.
A sneak peak at our 2016/2017 season:
On-line ticket sales now open for Fall 2016 shows »
"I Love You, You're Perfect, Now Change", Old Firehall Fall Show- September, 2016
"Shrek the Musical", Main Stage Production - November/December, 2016
"Jingle All the Way", TA Chorus - December, 2016
"Christmas at the Old Town Hall", Theatre Ancaster friends- December, 2016
"Valentine Love Notes With a Twist", February, 2017
"James and the Giant Peach", Stage 2 Jr. Broadway - February, 2017
"Fame the Musical", Stage 3 Youth - February/March, 2017
"The Birth of Rock & Roll", Main Stage Musical Revue - April, 2017
"We're in the Army Now", TA Chorus - May, 2017See our Playbill page for details »
Theatre Ancaster Season Sponsors
Full Season Sponsors - Nine Shows
Glendale Motors: Doug Kloet and Peter Boeringa
State Farm Insurance:
Steve Dunn
Eric Heyer: Ancaster Computer Products
Beckett Wealth Advisors: John, Jeffrey, and Jason Beckett
Judy Marsales Real Estate Ltd.
Season Sponsor - Three Shows
Brian Biemann, C.A. Professional Corporation
Full Season Restaurant Sponsor:
Present a ticket stub from a Theatre Ancaster production during the run of the show and receive a 15% discount on your food purchase.
1242 Garner Road W., Ancaster
Full Season Youth Program Sponsor:
Ancaster Orthodontics:
Dr. Melissa Sander
Click here for more about our sponsors »
Theatre Ancaster incorporated as a not for profit organization in 1997, and is also a registered charity.


text:
James Kingsbery
I am the Optimization Team Lead at Yodle, a company in New York City that does search engine marketing for local small-to-medium businesses. My team develops Yodle's proprietary bidding algorithm.
Before that, I was a software engineer for two startups located in New York.
My engineering specialties are Java, Maven and release process automation.
- New York, NY
- kingsbery.net
- Member for 3 years, 1 month
- 0 profile views
- Last seen Feb 23 '15 at 13:22
Keeping a low profile.
This user hasn't posted... yet.
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text:
Vt car search after robbery finds dummy grenadeThe Associated Press | September 04,2013MORRISTOWN Vermont State Police had a scare when they were searching a car after a robbery and found what appeared to be a hand grenade.
Police say they also found what appeared to be a gun and notes threatening to use the weapons during Fridays search, which followed Thursdays robbery at the Kinney Drugs store in Cambridge.
The car was parked outside the Morristown Police Department, which was ordered evacuated. A state police bomb squad was called in. The grenade turned out to be a dummy, and the gun turned out to be an air gun.
Police say they do not believe the case was related to the discovery of a live hand grenade outside a Morrisville restaurant Aug. 21.MORE IN Vermont NewsPHILADELPHIA After standing at the podium to second the nomination of Sen. Full StoryBARRE A Middlesex man could serve as many as five years in prison if he fails to complete drug... Full StoryPHILADELPHIA With his presidential campaign officially over, independent Sen. Full Story
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text:
In addition to the PlayStation Vita version, it looks like Phantasy Star Online 2 has found yet another platform: smartphones. The iOS and Android versions, of course, won't be exactly the same as the PC/Vita version, although you will be able to share data with those versions. The smartphone version, due out in winter of this year, will have simpler character creation, controls, and social game elements all for a simpler and more 'personal' experience. The first screens of this version are below:
You can see screens of the Vita version here. This version will be out in Japan in spring of next year.
As for the PC version, there's lots of screens from the closed beta and character creation demo, so we've added 63 of them to our gallery below. The closed beta is planned for late next month, and players will experience some of the story in the beta, too. Up to 100,000 testers will be invited. Meanwhile, the character creation demo will be released on April 5th. You can use your character from this in the beta test, actually, and test out how well your PC runs the game. SEGA also plans to hold a character creation contest soon.
The PC version of Phantasy Star Online 2 will launch first - this summer in Japan.
Tales from the Borderlands July 31 • 9:00am PDT/12:00pm EDT
Sword Coast Legends Aug 7 • 9:00am PDT/12:00pm EDT
Breath of Fire IV Continues! Every Monday • 5:00pm PDT/8:00pm EDT
Star Wars: KotOR Continues! Coming soon...


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Educator and philosopher Cornel West is the Class of 1943 University Professor at Princeton University. Known as one of America's most gifted, provocative, and important public intellectuals, he is the author of the contemporary classic Race Matters, which changed the course of America's dialogue on race and justice, and the New York Times bestseller Democracy Matters. He is the recipient of the American Book Award and holds more than 20 honorary degrees.
Award-winning writer David Ritz is the co-author of the autobiographies of Ray Charles, Marvin Gaye, Aretha Franklin, and B.B. King.


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Ravell Call, Ravell Call, Deseret News
SALT LAKE CITY — Most Utah voters support increasing income taxes to fund education, according to a new poll commissioned by Exoro and the University of Utah's Center for Public Policy and Administration.
The poll, released Wednesday, indicates that 55 percent of Utah voters somewhat or strongly support increasing income taxes, compared to 43 percent who somewhat or strongly oppose an increase. The question was worded to make clear that income taxes are used to fund education, which Robinson said likely contributed to the positive results, said Jennifer Robinson, director of the Center for Public Policy and Administration.
"When people understand how their tax dollars are spent, they're more supportive of increases," she said. "What you really see from the results here is the priority is on education."
The poll also shows that an overwhelming majority of voters — 88 percent — think it's important for the state to focus on education, compared to other issues like economic development, health care reform, immigration policy and transportation, which scored 82 percent, 66 percent, 59 percent and 46 percent respectively.
"It's just an enormous number," Robinson said. "That's almost to the point where you say it's a mandate from the public to focus on education."
When asked where specific education issues fall on voters' priority lists, 87 percent placed third-grade literacy at or near the top, followed in order of support by investing in public education, focusing on high school career readiness and reducing class size.
Poll participants were also receptive to other tax increases, with 54 percent strongly or somewhat supporting an increase in the sales tax and 50 percent saying they support returning the sales tax on food to previous levels.
The poll showed that voters were not willing to raise the gasoline tax to address transportation needs, with 51 percent strongly or somewhat opposed compared to 45 percent in support.
The poll was based on telephone interviews with 537 registered voters and has a margin of error of 4.5 percent.
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Andy writes about how he came to write romantic fiction having previously written crime and thrillers, and why he wouldn't have it any other way.
Exclusively for Foyles, Midge Raymond, whose debut novel My Lost Continent, is set in Antarctica, writes about Antarctica as a literary character in its own right.
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Filters: Keyword is ice [Clear All Filters]
Regional and interannual variability in sea level over 2002-2009 based on satellite altimetry, Argo float data and GRACE ocean mass." Ocean Dynamics 60 (2010): 1193-1204. Abstract"
Global Ocean Mass Variation, Continental Hydrology and the Mass Balance of Antarctica Ice Sheet at Seasonal Time Scale." Geophysical Research Letters 27 (2000): 3755-3758. Abstract"
Regional patterns of observed sea level change: insights from a 1/4° global ocean/sea-ice hindcast." Ocean Dynamics 59 (2009): 433-449. Abstract"
Sea-level rise at tropical Pacific and Indian Ocean islands." Global and Planetary Change 53 (2006): 155-168. Abstract"
Measuring the Distribution of Ocean Mass Using GRACE." Space Science Reviews 108 (2003): 331-344. Abstract"
Sea-level fingerprint of continental water and ice mass change from GRACE." Geophysical Research Letters 37 (2010): 19605. Abstract"
Future sea-level rise from Greenland's main outlet glaciers in a warming climate." Nature 497, no. 7448 (2013): 235-238. Abstract"
Spread of ice mass loss into northwest Greenland observed by GRACE and GPS." Geophysical Research Letters 37 (2010): 06501. Abstract"
Sustained mass loss of the northeast Greenland ice sheet triggered by regional warming." Nature Climate Change (2014). Abstract"
Patagonia Icefield melting observed by Gravity Recovery and Climate Experiment (GRACE)." Geophysical Research Letters 34 (2007): 22501. Abstract"
Stability of the West Antarctic ice sheet in a warming world." Nature Geoscience 4, no. 8 (2011): 506-513. Abstract"
Toward global inverse solutions for current and past ice mass variations: Contribution of secular satellite gravity and topography change measurements." Journal of Geophysical Research (Solid Earth) 107 (2002): 2291. Abstract"
Measurements of Time-Variable Gravity Show Mass Loss in Antarctica." Science 311 (2006): 1754-1756. Abstract"
Recent contributions of glaciers and ice caps to sea level rise." Nature (2012). Abstract"
Global time variations of hydrological signals from GRACE satellite gravimetry." Geophysical Journal International 158 (2004): 813-826. Abstract"
Interannual variations of the mass balance of the Antarctica and Greenland ice sheets from GRACE." Global and Planetary Change 53 (2006): 198-208. Abstract"
Postglacial rebound and Earth's viscosity structure from GRACE." Journal of Geophysical Research (Solid Earth) 107 (2002): 2376. Abstract"
Satellite Gravity Measurements Confirm Accelerated Melting of Greenland Ice Sheet." Science 313 (2006): 1958-1960. Abstract"
Greenland's contribution to global sea-level rise by the end of the 21st century." Climate Dynamics 37, no. 7-8 (2011): 1427-1442. Abstract"
Effects of ice melting on GRACE observations of ocean mass trends." Geophysical Research Letters 34 (2007): 05610. Abstract"
Accelerated Antarctic ice loss from satellite gravity measurements." Nature Geoscience 2 (2009): 859-862. Abstract"
Interannual variability of Greenland ice losses from satellite gravimetry." Journal of Geophysical Research 116, no. B7 (2011). Abstract"
Uncertainty in ocean mass trends from GRACE." Geophysical Journal International 181, no. 2 (2010): 762. Abstract"
Past rates of climate change in the Arctic." Quaternary Science Reviews 29, no. 15-16 (2010): 1716-1727. Abstract"


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Original Article
Quantification of DNA damage by q-PCR in cryopreserved zebrafish Primordial Germ Cells
Version of Record online: 12 NOV 2012
DOI: 10.1111/jai.12089
© 2012 Blackwell Verlag GmbH
Journal of Applied Ichthyology
Special Issue: Proceedings of the Third International Workshop on the "Biology of Fish Gametes" : Budapest, Hungary, 7–9 September 2011
Volume 28, Issue 6, pages 925–929, December 2012
Additional Information
How to Cite
Riesco, M. F. and Robles, V. (2012), Quantification of DNA damage by q-PCR in cryopreserved zebrafish Primordial Germ Cells. Journal of Applied Ichthyology, 28: 925–929. doi: 10.1111/jai.12089
Publication History
- Issue online: 12 NOV 2012
- Version of Record online: 12 NOV 2012
- Manuscript Accepted: 20 JUL 2012
- Manuscript Received: 2 MAR 2012
Funded by
- MICINN. Grant Number: AGL2009-06994
- Fundación Ramón Areces and Ramón y Cajal program. Grant Number: RYC-2008-02339
- Abstract
- Article
- References
- Cited By
Cryopreservation of Primordial Germ Cells (PGCs) for gene banking in fish is very promising considering the limitations that exist in fish embryo. Via germ line chimerism, these cells allow surrogate production in teleost fish. However, cryopreservation involves a range of extreme factors that might be stressful to cells. Moreover, Reactive Oxygen Species (ROS) produced during cryopreservation can damage DNA. Quantitative PCR was validated to quantify DNA damage mediated directly by reactive oxygen species or by cellular consequences of exposure to ROS. In this study, quantitative PCR (q-PCR) was used for the first time to measure mitochondrial and nuclear DNA damage after PGCs cryopreservation. Our data showed that the smallest number of lesions per 10 Kb was observed in one of the regions of the nuclear genome (2.6). However, nuclear genome was generally more sensitive than mitochondrial genome to damage induced by H2O2 or freezing without cryoprotectants. Using our cryopreservation protocol, the number of DNA lesions per 10 Kb was never higher than 4.68. In summary, q-PCR represents a feasible technique to quantify DNA damage, and this study demonstrates for the first time that it could be successfully applied to analyze PGC protocols.


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text:
Validation of formamide as a detubulation agent in isolated rat cardiac cells
Fabien Brette, Kimiaki Komukai, Clive H. Orchard
American Journal of Physiology - Heart and Circulatory Physiology Published 1 October 2002 Vol. 283 no. 4, H1720-H1728 DOI: 10.1152/ajpheart.00347.2002
Fabien Brette
School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, United Kingdom
Kimiaki Komukai
School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, United Kingdom
Clive H. Orchard
School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, United Kingdom
Validation of formamide as a detubulation agent in isolated rat cardiac cells
Fabien Brette, Kimiaki Komukai, Clive H. Orchard
American Journal of Physiology - Heart and Circulatory Physiology Oct 2002, 283 (4) H1720-H1728; DOI: 10.1152/ajpheart.00347.2002
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text:
The next chapter in the groundbreaking real-time strategy franchise from critically acclaimed studio Relic Entertainment. This standalone game features two unique armies with full length campaigns. Players will experience fast-paced dynamic tactics like never before, in two of the most brutal World War II campaigns ever fought.
Take command of the tenacious British 2nd Army in the vicious battle for Caen, France, or lead the German Panzer Elite as they struggle to repel the largest airborne invasion in history.
With two intensely cinematic campaigns, mission persistence, improved vehicle AI and weather effects that directly impact the battlefield, Company of Heroes: Opposing Fronts supports DirectX 10 and is fully compatible with the original Company of Heroes. Battle online with these two all-new armies and a total of four playable armies - allowing players to wage the ultimate war for Fortress Europe.
Good day comrades,
Today we bring you a new update about the status of the Eastern Front mod in Steam. It was a while since the last update, and while we have been busy with our own commitments outside this mod, but we are not dead and we will never be unless you read an announcement from us and you can be sure, if that moment comes you will read about it here firsthand!
Now about the content of the update, it'll be divided in sections to make it easier to read, so let's start:
1. Why the delay on the Steam version?
Besides our other commitments and obligations, we had a problem with our repository and we needed to set up another one, to those unware of that word, it means the place where we used to store the version in development from the mod was nuked so we lost the tool to centralize the changes and to avoid everyone having different changes due to the difficult of merging hundred of files, we stopped development until the server issue was resolved. Of course, since we were already busy to begin with, we delayed some time to set up a new one.
2.- When the Steam version will be released?
We had planned to release it near the current date, however, due to the situation mentioned above, the date is uncertain... however, you can be sure it will be released this year.
3. What about the models needed to replace?
We aren't finished on that, but we are closing the gap.
4. What has been changed on Eastern Front in all these months then?
So far, we have been working tunning the Soviet and Ostheer factions; we haven't made a total rework, so don't worry about having to re-learn how to use both factions. So about the changes:
The changes of the Soviets are subtle compared with the Ostheer ones, because the faction has been worked since long time ago compared to the latter. Soviets don't have new units so far, but they have received a lot of balance changes because of the feedback received in the beta.
We'll work on an updated teching system based on the feedback given from the beta players. Furthermore, the incorporation of Strafniki squads will be different from now - instead of being assault rifle infantry with ppd34 they'll receive SVT rifles.
There will be further tweaks of how the units are accessible in the future aswell as some unit availability switches.
Once the setup is clear we'll give further informations. Right now there's nothing specific.
Due to the feedback received so far, we are changing the Pool mechanic to make it better and also we are restructuring the tech path of the faction. Ostheer still has four advanced structures, however, only three of them produce units the fourth being a support-type building:
We have removed the Infantry Gruppen post, but some of their units were put back in the remaining buildings. We are also considering how to improve the current gameplay with Ostheer, due to their use of pool based assets according to the feedback received from the beta players.
The new building, the Luftwaffe Divisions Quarter, has six plane-based abilities to support the Ostheer commander being half of them reserved to support pool and the other half for assault pool. Some of these abilities are from the Support Army Troops tree, which means we would have to replace them.
The plan we have right now(remember it is not final), is to add one of the reward command trees to replace the Support Army Troops one, and we are discussing about which will be.
There are some new variants of the halftrack planned to be used for Ostheer:
The first three are going to be upgrades from the regular halftrack and we are planning to use the last one as call-in, although it is still WIP.
Furthermore, the pools no longer unlock units and the amount of total units has been reduced to streamline the faction and distinguish each unit from each other. Infantry units receive tweaks to their gear to fit them into their roles better aswell as making them more versatile in the course of the match.
The upgrades are connected to Assault and support pools. For example, Landsers when played in assault pool can upgrade MP40, while in Support Pool they get access to light MG34. Similar adjustments are made for all infantry units available to the Ostheer.
Another unit type that is heavily affected by the pools are now vehicles. The gunner/driver/commander vet system is tweaked, it no longer grants skirts or mg gunners to vehicles. Instead, each tank (StuG III, Panzer III, Panzer IV) receives the option to either upgrade Armour Skirts (Assault Pool) or Periscopes (with Support Pool) which give larger vision and weapon range to the vehicle. MG34 turret gunners are always available to the Ostheer commander.
Regarding the AI of the mod, Robotnik made tweaks so they tech faster the higher the difficulty. They also make better unit and upgrade purchasing decisions the higher the difficulty.
This would be all for this announcement, but if you have a doubt we can try to answer it in the comment section. As soon as we have all the details ready for both factions, we will let you know about it!
See you next time!
Archaic Entertainment Team
The first (will there be more? not sure) competition hosted by Packy21. (now closed)
This update contains few design changes in basic units and command tree.
The long awaited v4.0 update of Company of Heroes: Back to Basics is now available!
Battle of Crete v3.5 compatible with Company of Heroes (New Steam Version). Mod based on real historical event during WW2 between May 20 and June 1 1941...
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This is the Latest Far East Battlefield Mod v20160630. Released on 1 July 2016
This is the newest release, it's work with the Blitzkrieg V184.108.40.206. Enjoy it! Known bug is the status at the end of the game are poorly sorted.
This is the latest Blitzkrieg patch at the moment, to be installed over any 4.8.x.x version. Simply install it over and run launcher.
Last update for Non-steam version: Includes Africa, Italy, Normandy, market Garden, The Scheldt, Ardennes and Operation varsity.
The v4.1 update of Company of Heroes: Back to Basics is now available!
This is the newest release, it's work with the Blitzkrieg V184.108.40.206. Enjoy it! Known bug is the status at the end of the game are poorly sorted.
No files were found matching the criteria specified. We suggest you try the file list with no filter applied, to browse all available. Add file and help us achieve our mission of showcasing the best content from all developers. Join now to share your own content, we welcome creators and consumers alike and look forward to your comments.
can someone do a mod for transformers, it would be epic
thats ********
nobody wants it except you
Any updates for trainer? in Company of Heroes Complete Edition by SEGA.
because other sites are not for free.
I wish someone made a mod on the pacific front.
same, i really wish there was a pacific front mod, are there any good strategy games that focus on the pacific front during ww2
We should stay strong!
Only registered members can share their thoughts. So come on! Join the community today (totally free - or sign in with your social account on the right) and join in the conversation.
Highest Rated (9 agree) 9/10
This is more than Command & Conquer and Star Craft "Get the resources, build a shit-load of units and steam-roll your enemy" style of game. Not that there is anything wrong with that. I do like to play a 15-20min game with no "consequence" for loosing or wining, from time to time. But ever since Company Of Heroes came out...it's just amazing This game is way more realistic and in-depth than any other WW2 RTS game out there*.You have your account and as you play you get higher military rank and…
Nov 21 2010 by P3ACE753


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Fifth Circuit Contract Cases
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Shrieve Chemical Products, Inc. v. Caremoli
as 4:2016cv02173
Plaintiff: Shrieve Chemical Products, Inc.
Defendant: Andrea Caremoli, Caremoli USA, Inc.
Cause Of Action: Declaratory Judgment
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as 3:2016cv00484
Plaintiff: AmeriHealth Caritas Louisiana, Inc.
Defendant: Promise Hospital of Ascension, Inc.
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Defendant: Global Receivables Solutions, Inc.
Cause Of Action: Fair Debt Collection Act
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as 2:2016cv00929
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as 3:2016cv00405
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Defendant: Troy J. LeBlanc
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text:
Have Confidence
In Yourself
The Code helps you show self-doubt, second-guessing and people-pleasing who's boss.
It builds on what you already know, leverages your natural inner confidence (yeah, you have it) and takes you to a place where you're ready to get out there, be yourself and put your dent in the universe.
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When you graduate, taking your first steps out of College or University into the big, bad world is, how to put it... ...a little bit fucked up. The structure and support of your education and … Read More.


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Created using this Craftsy indie pattern
This project was made from an independent Craftsy designer's pattern:
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Quiltingdragonfly in the swamp
QuiltingSpring Pond pillow
QuiltingSpring on the pond my version
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Fantastic. Your fabric selections really bring this to life. Impressive paper piecing too.
04/04/2014 Flag
love it....great background choice
04/19/2014 Flag
Love it, the wings are beautiful!
04/19/2014 Flag


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Inside a function, one can put (for gcc)
printf("%s: I am here!", __FUNCTION__);
to indicate where a particular message comes from. Now, suppose I have serval functions A1, A2, A3,..., which all might call function B(). I am wondering if there is a neat way to indicate which function calls B() at run-time.