text:
Is your resume in need of a little disruption?
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making things work better.
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StartUpHire is the place to find Director-of-Data-Engineering jobs in and near . Review the list below of Director-of-Data-Engineering jobs. These Director-of-Data-Engineering jobs represent some of the most rewarding careers available. You can refine your job search for Director-of-Data-Engineering jobs in by adding a job title, company name, or keyword to the search box above … or click the "Advanced Search" link for more options. Don't see the employment opportunity you're looking for? Check back soon for more Director-of-Data-Engineering jobs in , or sign up for an email alert (link in upper left) to receive periodic updates on new Director-of-Data-Engineering jobs in , .
We're seeking a Sr. Director, Data Science to join our Data Science team in NYC.RoleAs a member of the Data Science team you will be solving complex problems across a wide range of areas related to causality, marketing attribution, ad viewability, online fraud detection, video7/4/2016 New York, NY Save This Job
Program Management Alameda, California APPLY Description Position at 24/7 Director Engagement Management ABOUT THE POSITION As part of the Platform Solutions Group (PSG), the Director of Engagement Management is responsible for leading a team of Engagement and Senior6/30/2016 Alameda, CA Save This Job
at PulsePoint New York JOB DESCRIPTION The Director is responsible for setting the strategy and pace of data-driven product innovation for the Data Science Team, while ensuring high standards of consulting excellence, proactive insights, and leveraging cutting edge7/1/2016 New York, NY Save This Job
San Francisco, CA Blurb is seeking a Director of User Experience Design who will work closely with stakeholders across product, engineering, marketing and customer service, to build amazing consumer products for the company. The ideal candidate will be a creative thinker,7/12/2016 San Francisco, CA Save This Job
Product Management Foster City, CA, United States About Conviva Conviva?s real-time big data platform enables content publishers and service providers to achieve a TV-quality viewing experience, despite the unpredictability of a complex and fragile Internet and device7/11/2016 Foster City, CA Save This Job
SAN MATEO JOB DESCRIPTION The Director, Data demands an analytical, detail-oriented self-starter who possesses strong data sense, project management skills and the ability to handle multiple tasks with confidence and maturity. This person must possess an understanding of latest6/27/2016 San Mateo, CA Save This Job
Product Management Foster City, CA, United States With a view into billions of streams per month from billions of unique devices, only Conviva can deliver online video providers the power to navigate the pitfalls of delivering video over the Internet. Our solutions record7/11/2016 Foster City, CA Save This Job
Global Services - San Diego, CA - Full Time JOB DESCRIPTION: Verimatrix is a high-growth software company that operates at the leading edge of the pay-TV industry, protecting movies and television from piracy, and our customers from theft of service. We are currently deployed at6/20/2016 San Diego, CA Save This Job
Please fill in the information below and use the navigational buttons to continue. Warning: Using your browser's Back and Forward buttons may produce undesirable results. SUMMARY: Reporting to the Sr Director of Engineering, the Principal Data Engineer is responsible for7/12/2016 Redwood City, CA Save This Job
Venice, CA Snapchat is seeking an exceptional leader for our growing team of Data Scientists focused on defining critical questions for the success of our product and business, analyzing rich sets of data, and generating powerful insights that directly inform our most strategic6/28/2016 Venice, CA Save This Job
Job Description Director, Product Management ? MedPage Today and DoctorDirectory - (16021) Description Everyday Health?s Professional Division is seeking a Director of Product Management to help lead the growth of our leading professional products MedPageToday.com and6/5/2016 Asheville, NC Save This Job
Location: Ann Arbor, MI Type: Full Time Min. Experience: Senior Manager/Supervisor Who We Are Deepfield is a fast-growing startup that builds the analytic and security solutions that monitor and defend over 90% of internet backbone networks. We produce cutting-edge research at7/14/2016 Ann Arbor, MI Save This Job
Finance/Accounting Austin, Texas Description Using data to improve processes and drive business goals something you love? CreditCards.com is looking for a passionate Data Analyst to turn data into information, information into insight and insight into business6/20/2016 Austin, TX Save This Job
Job Description Director, Product Management - (1602H) What you'll do: Everyday Health, a leading provider of digital health and wellness solutions. is looking for a Senior Director of Product Management to lead the consumer health units product strategy from ideation to6/5/2016 New York, NY Save This Job
Location:Boston, MA Job Code:554 # of Openings:1 Description Director of Product Management DataXu?s vision is to make marketing better using data science. The world?s top brands and agencies partner with DataXu to better understand and engage their customers across all7/11/2016 Boston, MA Save This Job
Requisition Number16-0030 Post Date6/30/2016 TitleDirector - Customer Support CityCarbondale, IL DescriptionDirector of Customer Support The Director of Customer Support for Liaison Technologies will report to the Senior Vice President of Customer Operations and serve a7/5/2016 Carbondale, IL Save This Job
Location: Seattle, WAType: Full TimeMin. Experience: Manager/Supervisor Our growing company, Rover.com, was named one of Forbes? top 50 startups of 2015. We are looking for passionate, hard-working individuals to join us in helping to make dog ownership possible for everyone. We6/4/2016 Seattle, WA Save This Job
Lead, Platform Engineering Meet Zuora. We?re a Micro Services, Docker, AWS, Redshift, and Java 8 shop that deals with extremely high volume data processing in the billions of dollars monthly. We are the market leader in subscription management helping companies transform from6/20/2016 Foster City, CA Save This Job
Recommind builds breakthrough software solutions that harness the power of information to solve concrete business problems. Our patented innovations at the nexus of law, big data, and predictive analytics help extract critical insights from massive volumes of unstructured data -6/18/2016 San Francisco, CA Save This Job
Department: Marketing Location: Boston, MA Director, Product Marketing, SaaS The Director, Product Marketing, SaaS at Acquia is the face of Acquia?s SaaS products?Acquia Lift, Acquia Content Hub, and Acquia Search?and primary advocate to the market. We are seeking an experienced7/26/2016 Boston, MA Save This Job


text:
Donnis' Break Hundreds & Hearts EP is out on iTunes now.
Donnis - Gimme (Video)
Wale Performs "My PYT" On 'Fallon'
Before the mixtape from Friday, Wale performs his next album's single on The Tonight Show.
Is A PARTYNEXTDOOR x Jeremih Project Coming?
According to one of them, yes.
Marion Write Hopes For Unity In Uplifting Flip Of Terrace Martin's "With You"
Denounce the hate, embrace the love.
DJ Mustard - "Don't Hurt Me" f. Nicki Minaj & Jeremih
Mustard returns with one of his more traditional offerings.

subset: commoncrawl


text:
(Colorado Music Festival / Courtesy photo)
classical musicAmong classical pillars, Colorado Music Festival offers hip-hop crossovers this week
The Colorado Music Festival's 2016 season is loaded with more events than ever, and the back end of the six-week musical smorgasbord is particularly rich in content and variety. Full Story
Review: Vadim Gluzman completes landmark week at Colorado Music Festival
07/22/2016 06:24 AM MDT - At the midpoint of the Colorado Music Festival season, world-class violinist Vadim Gluzman arrived, 1690 Stradivari in hand, to perform in three straight programs. The new SeiSolo Artist-in-Residence program allowed Gluzman to connect deeply with both the audience and the musicians. His comments from the stage have been affable, his performances transcendent. Full Story
'Tosca' is taut, tight and thrilling
07/20/2016 10:58 PM MDT - If you goWhat: Central City Opera presents Puccini's "Tosca." When: 8 p.m. Friday; 2:30 p.m. Sunday, Tuesday, July 30, Aug. Full Story
Review: Michael Christie cheered by Chautauqua crowd in Boulder
07/15/2016 07:15 AM MDT - Colorado Music Festival tonight: Concert at 7:30 p.m. with Orion Weiss performing chamber music at Chautauqua with cellist and CMF creative partner Joshua Roman. Full Story
Vadim Gluzman joins Colorado Music Festival for residency
07/14/2016 08:41 PM MDT - Among the many new aspects to this summer season at the Colorado Music Festival, the engagement of a highly prominent soloist as artist-in-residence for three concerts over the course of a week is one of the most exciting. Full Story
Review: 'The Ballad of Baby Doe' opens Central City Opera season
07/11/2016 11:13 AM MDT - A new production marking the 60th anniversary of the opening of "The Ballad of Baby Doe" opens the 2016 Central City Opera season. The troupe's deft handling of the opera's epic scope, which covers events traversing the last twenty years of the 19th Century, is made possible by technologies common enough today, but in their infancy in 1956. Full Story
Colorado Music Festival welcomes Michael Christie's return for concert
07/14/2016 04:39 PM MDT - When Jean-Marie Zeitouni was appointed music director of the Colorado Music Festival, it was understood that a guest conductor can be expected regularly in the second or third week of the festival. But the conductor for Thursday's large festival orchestra concert cannot in any sense be described as a "guest" to the Chautauqua stage. Full Story
Review: Zeitouni makes bold statement in first Brahms concert
07/08/2016 07:07 AM MDT - It may not have been the most original idea for Jean-Marie Zeitouni to present all four symphonies by Johannes Brahms on two consecutive nights. But it was a risky one. Full Story
Colorado Music Festival's Brahms cycle includes all four symphonies
07/01/2016 12:00 AM MDT - The two-night survey of the complete Brahms symphonies in the Colorado Music Festival's second week is part of music director Jean-Marie Zeitouni's vision for the festival's future. Full Story
Review: Colorado Music Festival's Zeitouni pulls out all stops in opener
07/01/2016 06:15 AM MDT - The Colorado Music Festival opened its 2016 season Thursday night at Chautauqua Auditorium, launching the second summer of symphonic concerts under the festival's third music director, Jean-Marie Zeitouni. Full Story
(Nicola Ianuale / The Denver Post)
Road to RioFairview grad Mara Abbott headed to Rio
In a sport like cycling, perseverance defines the course of any career. Mara Abbott, a Boulder-based Olympian, found that without the hardships she had to overcome to get to this point, she wouldn't be going to Rio de Janeiro this summer. Full Story
(Matt York / Associated Press)
College footballCU football: Buffs reeling from first blowout loss
Turnovers, sacks allowed pile up in defeats
After many of Colorado's Pac-12 losses over the years, there was a helpless feeling coming from the Buffaloes.Full Story
Second Story Garage: Bandits
Boulder is pretty good at producing rock bands, and by "rock," we mean the in-your-face, guitar-heavy, leather-clad variety — you know, the good kind. For a prime example, look no farther than BANDITS. Full Story
Local & National Video


text: Pluto just got denied planetary status AGAINRobbie Gonzalez1/29/13 6:20pmFiled to: GoofballeryMike BrownAstronomySpaceScienceScitweetFbPlutokillerCheer Up117EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalink Way over yonder in Berlin, Germany, the annual festival of media art and digital culture known as "transmediale" is just getting underway. This year's theme: BWPWAP (Back When Pluto Was a Planet).Mike Brown, the astronomer who killed Pluto, was invited to speak at the festival's opening ceremony, which included a debate over Pluto's demotion and a vote to reinstate its planetary status. Brown, of course, accepted, and has been documenting the festival on twitter. Here's where the festival is going down: Advertisement Judging from Brown's latest string of tweets, the Pluto debate is over, and the vote — conducted with yellow cards — has been carried out: Aaaaand here's the final tally on the Resolution to reinstate Pluto: Sponsored BOOM. Pluto is denied. Again. As it damn well should be. And Brown, twitter handle still in good order, proceeds to Paris to continue spreading the good word: All images via the Pluto Killer himself . Learn more about transmediale here . Read more about how Mike Brown killed Pluto and why it had it coming here Reply117 repliesLeave a reply


text:
This question already has an answer here:
I'm currently working with arrays and i decided to try and speed up one of my functions with replacing and iterative search of a sorted array with .include? much to my surprise the total run time of the program went from 2:12 to 9:53. confused by this i went to look at the source code for .include? and it turns out it's just an iterative check written in C
rb_ary_includes(VALUE ary, VALUE item)
long i;
for (i=0; i<RARRAY_LEN(ary); i++) {
if (rb_equal(RARRAY_AREF(ary, i), item)) {
return Qtrue;
return Qfalse;
is there a faster way to determine whether a certain item is in a sorted array, maybe a gem? or do things need to be done the hard way with writing in your own binary search method?


text:
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Sublimated shirts leave a permanent, vivid image with a soft-to-the-hand feel. Tees are made with high-quality, 100% spun polyester and deliver the look and feel of organic cotton with the wickability of poly-performance fibers, providing a great fit with a soft feel.
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text:
Perry Pagan
Published: Thursday, April 16, 2009 at 10:13 a.m.
Last Modified: Thursday, April 16, 2009 at 10:13 a.m.
Perry Clark Pagan, 52, a native El Dorado, Ark. and resident of Houma, died Monday, April 13, 2009.
Visitation will be from 5 to 6 p.m. Friday at Samart Funeral Home in Houma. Service will be at 6 p.m. Friday at the funeral home.
He is survived by his mother, Bonnie H. De Laneuville; one daughter, Charlotte Noel Zedlitze; two brothers, Harold Jr. and Drew Whit Pagan; and one grandson.
He was preceded in death by his father, Harold E. Pagan Sr.
He was a member of Christ Baptist Church.
Samart Funeral Home of Houma is in charge of arrangements.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.
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text:
Reviews written by registered user
|2 reviews in total
So this movie was not for everyone, I personally thought this movie was
An exorcism movie that does not involve the devil is refreshing to see, and the experience of the movie is played out quite well in the pacing and how events unfold. The fact it features the Jewish religion over Christianity is a very nice touch, one you rarely see in a movie of this style as most people hear exorcism and assume the Catholic church. While many may complain it is too slow, and other such things let me ask you this question.
What were you expecting from a movie that was purely plot driven?
Personally the acting was also great, I could empathize with the characters and understand them. The father was just worried about his daughter, who hid her signs of the problem well until things got bad. I mean when it really showed and she was hitting the kid at school, that looked like something that night happen in school over even something small like a favorite pencil or item. There was in my opinion no weak performances in the acting, coupled with a solid and strong story makes for quite a good movie.
Lastly the execution was some of the best I had seen this far, it flowed and was not jumpy like say the bourne movies. Nothing was out of place and the movie felt creepy and eerie throughout with all of the silence that you could almost hear in many parts of it. If your looking for a truly good movie that will have you walking away satisfied this is one of them.
It is a well written, acted, and executed movie that while it may seem slow moving is well worth the watch. Think of it what you will, but I recommend this movie as one of my top ten movies of 2012.
*** This review may contain spoilers ***
This is an honest review of this movie from me, I am not going to hold
anything back so please understand if I really hate an element of it I
will say so. First off the characters and I will try to keep it short,
simple, to the point.
Marty is my favorite character, I love how he portrays the fool and the stoner as he does an excellent job and is the only redeemable character in the movie. I actually agree with his philosophy that humanity needs a reboot, he is the only character that makes sense in this movie and properly uses his head even before the gas hits them. The fact the pot blend he uses makes him immune, I would be worried to if I were one of the people controlling this. He is the biggest threat to this plan, hands down.
The Jock is decently played but he is the scholarly kind, Chris Hemsworth is a great actor, though I am not a fan of stereotypes and how his character is also on a scholastic scholarship actually bends them. So no issues here, but I think his death coulda been different and more interesting than an evil kinevil stunt.
The slut just plays it as mentioned before, however I liked her before the gas and after it I just despise her. I am not surprised she is among the first to go, cliché there and this kinda lame death too. Unimpressed with this character, but it's catering to the typical audience. I do not even think the actress who plays her is even pretty, then again I do not have a thing for celebs as their more trouble than their worth.
The virgin acts so naive that it's not even funny, seriously her role is the lamest of the bunch. I am sick of horror movie "virgins" acting so naive and stupid, and walking into the scenario like a blind man walking into the pit. Her character annoys me the most, which makes her the most unlikeable.
The scholar is OK, nothing much to say about him except that he should have the common sense even under the influence of the gas to know what is going on. Honestly he is my third favorite character after the fool and the jock. The jock himself could have easily passed for the scholar, so this role is kind of redundant except they needed 5 sacrifices.
For characters I give this movie a 4/10, Marty saves it as I love his character.
Plot: The plot as mentioned before is rather lackluster, while the concept is good it is poorly executed and this is my honest opinion. Joss Whedon has done much greater things in Buffy, ANgel, Firefly, Serenity, Dollhouse, and other series as well as movies. I know this is supposed to be a satire but I'm not laughing, I am rather bored at how the character interaction seems to develop awkwardly and none of the characters can really relate to each other unless manipulated. The first half of the movie is by far the worst part, only until the ending does this half baked and mediocre plot actually show promise.
One would think the absence of stars would be a bad thing, honestly if I saw that I'd be assuming something is up and trying to find the cause immediately. The whole feel of the movie leading up to the ending is like they tried to cram too much into the short span of the movie at once. This movie felt rushed and thus the poor execution, and if anybody suggests splitting up in a setting like this I too would be immediately pushing to stay as a group and force my opinion on the group.
The ending however is genius and the only saving grace, almost all movies end up with survivors and they move on to live disturbed but productive lives. Happy ending somewhat, this movie takes that cliché and burns it in the fire. So there is little to this movie besides the ending, that I enjoyed plotwise. How the fool survives being killed is beyond me, and leaves a huge and glaring plot hole. You don't see them die but also you do not see them escape, which leaves me to wonder if this part was not thought through too well. Honestly come on Joss, you can do better than this kind of shoddy writing that leaves holes in the plot where it seriously looks and sounds like someone died.
Clichés are good and all IF they mesh together well, but honestly their over-saturating this film and there seems to be no escape from them in Hollywood today. So overall for plot I'd give this movie a 3/10, not horrible but not even average or sub par. This is sub-sub-par and in my opinion a movie to watch as background noise.
Now as for the quality of acting, what can one expect? With headliners like Chris Hemsworthm Fran Kranz, and others this has decent acting. Though it is stereotypical and a satire I still think they could have done way better, but the acting is nothing special and very typical. I give the acting job a 2/10 with the exception of Chris Hemsworth and Fran Kranz. Amy Ackers is also great, though she has such a minor role in it, seriously she should have had more of a role in my opinion.
Overall I give this movie a 3/10 rating as it could have been way better even for a satire, but due to many factors it fails as a movie. This also rates as being classified at background noise, a movie to watch while your doing something monotonous to break up the tedious nature of whatever your doing with something to distract your eyes.


text:
[edit] Background
Banjo and Kazooie take to the skies again with Banjo Pilot. Pick out your favourite from an unlikely squadron, each bringing along their own customisable flying machine, then launch yourself headlong into 3D courses. You can even link up with three friends, competing for the title of squadron leader or simply blast each other out of the sky in frantic dogfights.
[edit] Features
- Unmatched handheld air racing with fast, fluid gameplay and soaring, sweeping action
- Choose from different pilots from the Banjo-Kazooie series, each with their own unique stories and custom aircraft
- Choose from tracks filled with obstacles, power ups, and numerous opponents
- Game Boy Advance Game Link cable support for up to four players


text:
First trailer of UT3 360 released, game dated as "summer"
See it after the link. As you can see, at the end of the video a man who clearly smokes to much pegs the 360 version of Epic's shooter for a summer release date. According to this IGN piece, it's going to have split-screen and five new maps over the PS3 and PC versions. The game's being shown off at Midway's Las Vegas event this week, so expect plenty of playtests in the not-too-distant future.
Sometimes we include links to online retail stores. If you click on one and make a purchase we may receive a small commission. For more information, go here.


text:
Nir Shaviv
Nir J. Shaviv
- Ph.D., Physics. Israel Institute of Technology, 1996.
- M.A., Physics. Israel Institute of Technology, 1994.
- B.A., Physics. Israel Institute of Technology, 1990.
Source: [1]
Jump to navigation
Justin Trudeau and his cabinet must uphold their promise to respect First Nations rights when it comes to federal decision-making for the ...
The UK's largest pension company will call on the government to discuss legislative backing for clean energy investment later this year.
The University...
Source: [1]
Source: [1]
Dr. Harrison Hagan "Jack" Schmitt is a geologist who worked as a scientist-astronaut with NASA from 1965 to 1975. He is noted as being the last of the Apollo astronauts to arrive and set foot on the Moon.
Roger Cohen is the former Manager of Strategic Planning and Programs at ExxonMobil Corporation.
He is a George C. Marshall Institute "Expert," and has led a push for the American Physical Society (APS) to weaken their position on climate change.
Cohen became involved in studying global warming when Exxon realized that its "business environment" could be affected. [2]
Source: [1]
Jan L. Breslow is a physician and professor at Rockefeller University, specializing in cardiovascular disease. He is a former president of the American Heart Association. [1]
Source: [1]
Antonino Zichichi is an Italian physicist who specializes in the area of nuclear physics. He was president of the Istituto Nazionale di Fisica Nucleare from 1977 up to 1982 and is currently an emeritus professor of physics at the University of Bologna. [2]
Claude Allègre, 75 years old, is a French geochemist and politician. He is a former minister of the French Socialist Party, [2] and does academic work at the Institut de Physique du Globe de Paris (IPGP) — Institute of Geophysics, Paris.
Source: [1]
Source: [1]
Edward David is an American electrical engineer.


text:
Broccoli Vinaigrette
|Frozen broccoli/Watercress
|1 Pound
|4 , skinned
|Salad oil
|3 Tablespoon
|1 Tablespoon
|Ground black pepper
|To Taste
|Dry mustard
|1 Pinch
|To Taste
1) In a pan boil salted water and cook broccoli till done.
2) In a bowl whick dressing ingredients.
3) Drain broccoli and pour the dressing on top when still hot.
4) Keep aside and cool.
5) Serve on a bed of lettuce or watercress. Scatter chopped tomatoes on top. Serve with cold meats.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 626 Calories from Fat 406
% Daily Value*
Total Fat 46 g70.9%
Saturated Fat 2.2 g11.2%
Trans Fat 0.8 g
Cholesterol 0 mg
Sodium 546.3 mg22.8%
Total Carbohydrates 41 g13.8%
Dietary Fiber 16.9 g67.4%
Sugars 13 g
Protein 20 g41%
Vitamin A 348.9% Vitamin C 798.2%
Calcium 26.8% Iron 9.1%
*Based on a 2000 Calorie diet


text:
Grey shorts for men made of 86% polyester and 14% spandex, engineered with Dri-Fit technology, has an in-built boxer inner, elasticated waist with a drawstring, side meshed panels with white piping, a zipped pocket at the back and printed nike swoosh on the left hem
Wash care
Machine wash cold at 30 degrees with like colours
Do not bleach, dry clean or iron
Line dry
Stylish to a T, these polyester shorts from nike are designed with Dri-Fit technology to keep you fresh and comfortable while you sweat it out at the gym or on the trail. The broad waistband sits snugly, while you can further customise the fit with the drawstring. The in-built boxers offer greater comfort. Team it with Nike Sports tees and shoes for that smart, sporty look.


text:
White Hinterland: 'The Destruction Of The Art Deco House'
"The Destruction of the Art Deco House"
White Hinterland makes hazy pop music perfect for a rainy day. The group from Jamaica Plain, Mass., is fronted by singer Casey Dienel who's got a soulful, bluesy voice. White Hinterland's new CD is Phylactery Factory, a soothing, folk-flavored album with shades of chamber pop and jazz.
Phylactery Factory opens with "The Destruction of the Art Deco House," a song with a quiet ambiance that highlights Dienel's unique voice. The song moves from a sullen jazzy introduction to haunting experimentation with various string instruments and Dienel rumbling a piano.
Phylactery Factory gives a unique twist to jazz and folk with a range of unconventional instruments such as saw, mellotron and euphonium.
Despite having gear stolen after a recent gig, White Hinterland's future tour plans look strong, as they will be supporting Bon Iver and fellow labelmates Phosphorescent for a spring tour.
Featured Artist
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text:
I'm using the current version of both WordPress and Suffusion.
I have created a new page, but can't seem to find how to disable the "Comments" section.
Can someone tell me how to do that?
I'm using the current version of both WordPress and Suffusion.
I have created a new page, but can't seem to find how to disable the "Comments" section.
Can someone tell me how to do that?
Hello, to remove easily comments from posts, go to main menu: "all posts" and "quick edit" of each post; uncheck "allow comments" and if un wanted too, "pingback". Work the same for "pages".
Since a number of my sites are made for others, I don't like adding yet another thing for them to remember to do when they make a post.
For a site where comments are not wanted, it's not enough to change the settings in the Discussion menu. That gives you just the ugly "Comments are close" message under every post. I find I need to create a child theme in which the PHP code for commenting is edited out of index.php, single.php, and page.php.
I confess I sometimes have to do a bit of experimenting to get it just right. :-)
To remove the comments are closed message simply go to Suffusion options---------then select backend-------- then scroll down to ' Hide "Comment form closed" Message on Posts '------tick the hide the message box-----------save,--------- and you are done.
Keep in mind that comments are content and indexable by the search engines so can be useful to have.
They can however be unsightly on sites like my woodblock print site.
Rather than turn them off I'm thinking of creating a child page and having comments there and link to it from the parent/gallery page. That way I keep the content but it doesn't clutter up the appearence of my gallery pages.
Hi Michael,
Thanks for your comment. You are quite right, comments are indexable by search engines and are therefore very useful but are, for some people, unnecessary and, as you say, unsightly.
I don't know about creating a child page, perhaps you might think about adding a forum with a direct link from your gallery; after all there are many old Japanophiles like myself who would enjoy the the 'banter' between former residents of Tokyo.
By the way I love your site, might I ask if your gallery is a simple plugin or is it bespoke? If the former I would greatly appreciate a link.
Hi Will,
I use the NextGEN plugin for the gallery and Suffusion theme (using Minima skin) with a little bit of CSS to tailor the NextGEN grid view appearence.
Regrads, Mike.
Hi Mike,
Many thanks for the info.
This topic has been closed to new replies.


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Whispering Grass
"Whispering Grass (Don't Tell The Trees)" is a popular song written by Fred Fisher and his daughter Doris Fisher. The song was first recorded by Erskine Hawkins & His Orchestra in 1940. The Ink Spots featuring Bill Kenny also recorded it the same year. A live instrumental version was played and recorded by Johnny Hodges with Duke Ellington and his orchestra in the Cristal Ballroom, Fargo, North Dakota, also in 1940.
The words of the song are reminiscent of those of Kahlil Gibran: "If you reveal your secrets to the wind, you should not blame the wind for revealing them to the trees."[1]
"Whispering Grass" was a 1975 UK number-one single by Windsor Davies and Don Estelle.[2] Davies and Estelle were actors in the successful sitcom It Ain't Half Hot Mum. It was number one in the UK Singles Chart for three weeks from 7 June 1975.[3] The record is a comic version of The Ink Spots' 1940 recording, and was sung in character.
Other versions[edit]
The song was also recorded by:
- Ringo Starr on his 1970 album Sentimental Journey.[4]
- Sandy Denny on her 1973 album Like an Old Fashioned Waltz.
- Kay Starr on her 1977 album Cry By Night/Losers Weepers.
- Kat Edmonson on her 2012 album Way Down Low.
- The Skatalites, as 'Whispering Dub' on their Herb Dub Collie Dub album first issued in 1976, re-issued in 2001.
- ^ Gibran, Kahlil. "goodreads".
- ^ Roberts, David (2006). British Hit Singles & Albums (19th ed.). London: Guinness World Records Limited. p. 314. ISBN 1-904994-10-5.
- ^ UK Chart Stats
- ^ Miles, Barry (1998). The Beatles a Diary: An Intimate Day by Day History. London: Omnibus Press. ISBN 9780711963153.
External links[edit]
|Preceded by
"Stand By Your Man" by Tammy Wynette
|UK number one single
7 June 1975 for three weeks
|Succeeded by
"I'm Not in Love" by 10cc
|This 1970s single–related article is a stub. You can help Wikipedia by expanding it.


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Germany's Loans
NPR's Edward Lifson reports that German stocks, particularly banking shares, are sharply down because of the Russian financial crisis. German banks have made some 30 billion dollars in loans to Russia, backed by the Bonn government. Analysts say, however, that even a complete default on the Russian loans would not create financial upheaval in Germany. Nevertheless, Chancellor Kohl's challenger in the upcoming federal election, Gerhard Schroeder, is making political hay out out of the current financial turmoil.
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Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research.
Protein patterning is becoming increasingly important for a myriad of applications in biosensors (Ekins 1989; Wang et al. 2007a; Zhu and Snyder 2003), developmental biology, stem cell biology, and tissue engineering (Fischbach et al. 2007; Herbert et al. 1997; Khademhosseini et al. 2006; Shen et al. 2008; Von Philipsborn et al. 2006). It is generally accepted that chemical and molecular cues play a critical role in cell differentiation and migration during early embryonic development (Gurdon and Bourillot 2001). However, applying this property for tissue engineering applications has been thus far limited, primarily due to the technical constraints in producing gradients of molecular cues or cell binding ligands as close as native extracellular matrix environments. On the other hand, it is also generally acknowledged that other factors including the topographic and mechanical properties of the environment can affect cell development (Clark et al. 1991; Engler et al. 2006; Janmey and McCulloch 2007). As an example, both molecular (Nishiyama et al. 2003) and topographic (Clark et al. 1991) cues can guide axon growth in tandem. It is difficult to identify specific contributions of each factor during certain cell developmental stages due to technical limitations in reproducing the effects with comprehensive microenvironment control ex vivo.
While many protein patterning techniques, including contact imprinting (Choi and Newby 2003; Von Philipsborn et al. 2006), diffusion processes (Dertinger et al. 2002; Georgescu et al. 2008; Jeon et al. 2000; Luo and Shoichet 2004; Wang et al. 2008), and optical lithography (Herbert et al. 1997; Hypolite et al 1997; Li et al. 2005) are available, each of these methods has technical limitations that restrict its usefulness for biological research. Close approximations of in vivo biological environments require that certain characteristics of protein patterns be considered. First, in order to study cellular processes that occur over days or weeks, stable protein patterns created using covalent binding is preferred over physical adsorption methods (Zhu and Snyder 2003). Second, molecular cues or cell binding ligands should be presented as continuous gradients that are convenient to design and reconfigure. Many contact imprinting methods only produce discrete dots that can be patterned to form a gradient density (Von Philipsborn et al. 2006). Diffusion processes, e.g. integrated microfluidic channels (Dertinger et al. 2002; Georgescu et al. 2008; Jeon et al. 2000; Wang et al. 2008), are able to produce continuous gradient protein patterns, however they often require new microdevice design and fabrication to reconfigure the gradient profile. Third, as mentioned above, mechanical and topographic properties of the cell culture environments also play very important roles in cell development (Clark et al. 1991; Engler et al. 2006; Janmey and McCulloch 2007, Mai et al. 2007). Therefore, a flexible protein patterning method is desired; one that can be used on traditional cell culture substrates (e.g., glass and tissue-culture polystyrene) and that has mechanical properties similar to the native ECM environment. The optimal method would be highly efficient to enable high throughput patterning and screening of multiple microenvironments. While scanning optical lithography is a very successful way to produce protein gradients in polymer environments, (Herbert et al. 1997; Hypolite et al 1997; Li et al. 2005) it is hampered by its relative slowness. Finally, cells in vivo grow, migrate, and differentiate in three-dimensional (3D) microenvironments, however many in vitro protein patterning methods are limited to patterning of two-dimensional (2D) substrates. In this paper, we present a novel protein patterning method that addresses each of the limitations described above. We demonstrate a simple dynamic mask lithography method to fabricate any user-defined 2D or 3D gradient pattern from an artificial extracellular matrix (aECM) protein. The cell-binding domains are covalently incorporated into the aECM environments and produced in continuous gradient patterns. This is a high throughput optical lithography method where the pattern shapes and gradient profiles can be conveniently reconfigured. We test the generated patterns and show them to be compatible for cell cultures.
In order to develop a flexible protocol capable of fabricating both 2D and 3D patterns of arbitrary geometry, we employed a dynamic mask projection lithography system that has been designed previously for microstereolithography applications (Lu et al. 2006; Sun et al. 2005). This system utilizes a Digital Micromirror Device (DMDTM, Texas Instruments) as a dynamic lithography mask instead of a conventional, permanent mask or other scanning methods. This lithography process offers several distinct advantages over conventional masks or scanning protocols including: parallel patterning, high throughput prototype development, and particularly easy procedures for obtaining any desired gradient via gray scale lithography. Another major advantage of this system is the high resolution, 3D complex structures that can be fabricated (Lu et al. 2006; Sun et al. 2005; Zhang et al. 1999). Due to the parallel processing capabilities of DMD, the applications have been extended to parallel manipulating, sorting, and stimulating of cells (Chiou et al 2005; Wang et al. 2007b). However previous reports regarding the applications of this fabrication tool have been primarily limited to photo-sensitive, synthetic polymer materials. Because proteins are not intrinsically photoactive and are often denatured or degraded by the reagents commonly used in photolithographic processing, protein patterning using dynamic mask lithography has not been previously described.
Recent reports have demonstrated that a genetically modified strain of Escherichia coli is capable of synthesizing intrinsically photoactive proteins that can be directly patterned through optical lithography (Carrico et al. 2007). This technique has been used to synthesize a family of artificial extracellular matrix proteins (designated as aECM-N3) with partial replacement of the canonical amino acid phenylalanine with the photosensitive non-canonical amino acid para-azidophenylalanine (pN3Phe) (Carrico et al. 2007). Multiple cell-binding domains, such as the CS5 or RGD sequences derived from fibronectin (Heilshorn et al. 2005, 2003; Liu et al. 2004) can be designed and inserted into the aECM-N3 proteins to promote cell adhesion through specific cell-surface integrin receptors. Additionally, the elastic moduli of the photocrosslinked aECM-N3 protein film can be modulated through the ratio of pN3Phe incorporated and through the intensity and duration of light exposure. Taken together, these results demonstrate that aECM-N3 photocrosslinked scaffolds have similar mechanical and cell binding properties to native extracellular matrix proteins, making them ideal materials for potential use in medical devices, drug and cell delivery vehicles, and tissue engineering scaffolds (Carrico et al. 2007).
By combining dynamic mask projection lithography and protein engineering with non-canonical photosensitive amino acids, we are able to create both 2D and 3D protein patterns with cellular-level resolution using engineered aECM-N3 proteins.
Engineered Escherichi coli (designated AF-IQ pNS-CS5-ELF) harboring the aECM-N3 plasmid and capable of replacing the canonical amino acid phenylalanine with the non-canonical amino acid p-azido-phenylalanine (pN3Phe) was a kind gift from Professor David Tirrell Caltech laboratory (Carrico et al. 2007). Primary amino acid sequence of the aECM-N3 proteins is shown in Fig. 1a. Cultures were grown in minimal medium supplemented with the 20 canonical amino acids (20 mg/L each except 12 mg/mL phenylalanine). Expression of aECM protein was induced at OD600 of 0.8-1 with 1 mM isopropyl-β-D-thiogalactoside. Ten minutes post-induction, pN3Phe (final concentration < 250 mg/L) was added. Cells were harvested 4 h post-induction and protein production was monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the whole cell lysate, using an HRP-conjugated T7-tag™ antibody. Cell pellets, produced by spinning down (10,000g, 10 min, 25°C) 1 L of expression culture, were resuspended in 20 mL of TEN buffer (10 mM Tris, 1 mM EDTA, 0.1 M NaCl) by sonication and subsequently frozen. 1 mM PMSF and 10 μg/mL each of DNase and RNase was added to frozen lysate. This mixture was agitated for 4 h at 37°C. Target protein was partitioned to the pellet via centrifugation at a temperature above the expected LCST of the protein (22,000g, 60 min, 25°C), and subsequently extracted from the pellet into 4 M urea at 4°C. This suspension was clarified by centrifugation (22,000g, 60 min, 2°C). The resulting supernatant was exhaustively dialyzed in cold (4°C) distilled water and subsequently lyophilized. Typical experiments yielded 40 mg of protein per liter of culture.
The principle of the gradient lithography of aECM-N3 proteins is shown in Fig. 1b. For 2D lithography, the aECM-N3 proteins were dissolved in water (100 μg/μl, 4°C) and spin-coated at 1,000 rpm onto an aminated glass coverslip at 4°C. Clean glass coverslips were prepared by sonication in ethanol saturated with potassium hydroxide for 15 min. The slides were then washed with water and aminated in a solution of ethanol-acetic acid-diethyldiaminotriethoxysilane (93:5:2) for 15 min. Aminated surfaces were washed thoroughly with deionized water and dried under a stream of argon. For 3D lithography, the aECM-N3 proteins were dissolved in dimethyl sulfoxide (DMSO, 100 μg/μl, RT) and pipetted into the center of a glass coverslip (20 μl on an 18-mm round coverslip), surrounded by a thin circle of vacuum grease, and sandwiched with a rectangular coverslip (24×36 mm) to a separation thickness of 2 mm. The vacuum grease applied at the edges provided a good seal to prevent evaporation.
The dynamic mask projection lithography system is shown as Fig. 2. The gray-scale patterns can be easily designed and generated using commercial software like Matlab (Mathworks, Inc) and Photoshop (Adobe Systems, Inc). These images were then loaded and projected on the aECM sample using a DMD with UV (ultraviolet) LED (Light Emitting Diode) at 380 nm as the light source. This procedure allowed the gradient modulation of UV light intensity on the sample. The reduction ratio from DMD to sample was from 10× to 4× for different spatial resolution and lithography area requirements. For different reduction ratios, spatial resolution varied from 2 μm to 4 μm and the corresponding lithography area varied from 2 mm to 4 mm, light power density on the sample plane varied from 1 to 0.2 mW/mm2. The exposure time for spun-on aECM-N3 sample was 3 to 5 min, for the liquid film sample, it varied from 5 min to 20 min for the reduction ratios from 10× to 4×.
Details of the DMD projection lithography system have been described previously (Sun et al. 2005; Wang et al. 2007b). After irradiation, samples were washed three times by agitation in 0.1% Sodium Dodecyl Sulfate (SDS) for a minimum of 4 h, thoroughly rinsed with water, and stored in PBS at 4°C until further use.
To visualize the 2D protein patterns, the T7 tags at the N-termini of the engineered proteins were specifically labeled using fluorescent antibodies. Briefly, samples were blocked with 1 ml 10% bovine serum albumin in PBS for 30 min followed by addition of 0.5 μl T7 tag-primary antibody and 6 h incubation at room temp. Samples were rinsed three times with PBS for 5 min, then blocked with 1 ml 10% bovine serum albumin in PBS for 30 min. Secondary antibody (100 μl of anti-mouse-Cy2 reconstituted to 1 mg/ml with water and then diluted with an equal volume of glycerol) was added and incubated for 2 h, samples were rinsed four times with PBS, 5 min each. Then the patterns were imaged using a confocal microscope.
For characterization of the 3D lithography protein patterns, the hydrated protein patterns were imaged in dark field by an inverted optical microscope (TE2000, Nikon). Then the sample was dried and its topographic profile was measured by the profilometer (Alpha-Step IQ Surface Profiler). After 20 nm gold was coated through sputtering, the sample was characterized by SEM (quanta 200 HV from FEI Company).
In order to get the elastic moduli of crosslinked aECM-N3 protein, a 1 mm thick layer of crosslinked protein film was made and characterized in PBS solution by AFM (Dimension 3,100, Vecco). The standard AFM force curve measurement was performed on the full hydrated protein sample with a relative blunt tip (cantilever spring constant 0.6N/m, NSC19/Cr-Au, MikroMasch). The elastic moduli were estimated through the slope of the AFM force curve (Dimitriadis et al. 2002).
PC-12 cells (ATCC, Manassas, VA) were cultured in PC-12 complete growth media (F12 Kaighn's media, 10% horse serum, 5% fetal bovine serum, and 1% penicillin-streptomycin) at 37°C, 5% CO2. Cells were differentiated in PC-12 differentiation media (F12 Kaighn's media, 1% penicillin-streptomycin, 50 ng/mL recombinant human β-NGF (R&D Systems, Minneapolis, MN)) at 37°C, 5% CO2. Media was changed every other day for all cell experiments. aECM-N3 patterned substrates were soaked in poly-L-lysine for 1 h to promote cell adhesion. PC-12 cells were seeded directly on the 3D patterned substrate and cultured for 48 h in complete growth media followed by one week of culture in differentiation medium. Cultures were then washed 3× with PBS buffer, stained with 1 μg/ml DAPI (Roche) and immunostained with neuronal class III β-tubulin (Tuj1) primary rabbit monoclonal antibody (1:250, Covance, Berkeley, CA) followed by secondary antibody (goat-anti-rabbit) conjugated with Alexa Fluor 488 (1:500, Invitrogen, Carlsbad, CA). Immunostained samples were fluorescently imaged with an inverted Zeiss Axiovert 200 microscope (Oberkochen, Germany, 20× objective) and a CCD camera.
Figure 3 shows a representative 2D gradient protein pattern made using the DMD projection lithography system. The aECM-N3 protein (100 μg/μl in water, at 4 degree) was spin-coated at 1,000 rpm onto a glass coverslip, resulting in a film around 450 nm thickness (Carrico et al. 2007). The sample was exposed to a gray-scale mask image (Fig. 3a) generated by the computer via the DMD. This mask figure was a linear gray-scale gradient pattern with a grayscale range from 0 to 255 for the individual pixels enclosed by a bright rectangular box outline (pixel intensity of 255). Upon irradiation, the aECM-N3 protein underwent covalent chemical crosslinking both to other aECM-N3 proteins and to the aminated glass substrate (Fig. 1). To develop the pattern, the substrate was simply rinsed in a suitable buffer to remove uncrosslinked protein from the substrate. Standard immuno-histochemistry was utilized for fluorescence labeling of an N-terminal peptide tag present on each protein chain (Fig. 3b). The image distinctly shows the gradient protein profile, enclosed by a rectangular box which is also present in the design. Because the spun-on aECM-N3 layer is very thin, this gradient can be approximated as a two-dimensional surface concentration gradient of aECM. Quantification of the fluorescence intensity demonstrates excellent fidelity to the gray-scale dynamic mask (Fig. 3c). Each gray scale pixel here corresponds to 3.4 µm in the sample pattern. This method represents a simple and efficient procedure for designing and creating user-defined concentration gradient protein patterns that can be easily calibrated. Rough features in the gradient protein pattern and noise in the characterization curve (Fig. 3b, ,c)c) are presumably due to aggregates of the aECM-N3 proteins or the fluorescently labeled antibody detection reagents. In general, this is a very convenient method for making 2D concentration gradient protein patterns by projecting gray-scale mask images via DMD; the parallel nature of DMD dramatically improves throughput compared to light scanning lithography techniques. Additionally, since the aECM-N3 proteins form covalent linkages when exposed to UV, the fabricated gradient pattern is very stable for studying long term cell cultures.
3D gradient protein patterns were achieved using aECM-N3 proteins and a dynamic mask protocol. A solution of aECM-N3 proteins (100 μg/μl in DMSO) was sandwiched between two coverslip substrates with an approximate 2 mm thick spacer. The irradiating UV light was focused at the substrate-solution interface for exposure, and then the coverslips were separated and rinsed in phosphate buffered saline (PBS) for 7 days. The elastic modulus of a fully hydrated and crosslinked sample depends on the ratio of incorporated pN3Phe (Carrico et al. 2007). Using AFM measurements, we estimate the value of this parameter to be in the order of ~ 102 kPa, which is within the appropriate bounds for testing cellular responses to mechanical and topographic environments (Engler et al. 2006; Janmey and McCulloch 2007). Using this protocol, we made a gradient structure for cell culture testing with periodic-ridges using a dynamic gray-scale mask as linear pixel gray-scale gradient lines from 0 to 255 with 200 μm periodicity. The scanning electron micrograph of the dried crosslinked aECM-N3 protein shows a clear repeating pattern of 3D gradient ridges, Figure 4a. A profile of the cross-section topography measured by profilometer (Alpha-Step IQ Surface Profiler) indicates the ridges have a 200 µm pitch in periodicity, as designed, and that the ridges form 3D gradients that gradually increase in height, peaking at about 20 to 30 µm, Fig. 4b. We estimate the fully hydrated ridge height should be about 40 µm high, due to swelling during hydration (Carrico et al. 2007). In order to demonstrate the flexibility of this method to make any potential geometric shape for various potential applications, two other 3D topography gradients were fabricated with non-linear geometry. Figure 4c and d display repeating structures with zigzag and semi-circle patterns respectively, with precisely controlled distances between the structural repeats. In these examples, the height, topographical curvature, variety of shape, and periodicity of the aECM-N3 protein patterns were designed to have length scales appropriate for future studies linking topography cues to cell development (Mai et al. 2007).
We tested the biocompatibility and cell responses to the 3D aECM-N3 patterned gradients. PC-12 cells, a neuronal-like cell line, were cultured on ridge patterns similar to those in Fig. 4a but with varied ridge width and periodicity and coated with poly-L-lysine (PLL) to initiate non-specific cell adhesion. Cells were found to adhere, differentiate, and extend neurite-like processes on both the ridges and in the troughs of the 3D substrates (Fig. 5a and andb,b, respectively). For cells growing both on ridges and in troughs, the neuritic processes tended to grow parallel to the pattern direction and align along the ridge and trough edges where the topographic curvature was relatively large (arrows in Fig. 5). The aECM-N3 scaffolds employed in this study contain the CS5 cell-binding domain derived from fibronectin, a ligand for the α4β1 cell-surface integrin receptor, which is generally not found on neuronal cells. Negative control experiments confirmed this hypothesis, as PC-12 cells were unable to adhere to aECM-N3 films without PLL coating (data not shown). Therefore, changes in cell morphology observed on these patterned structures are attributed solely to the 3D topography gradient and not to potential variations in local cell-binding domain density. These results demonstrate that our materials and fabricated protein patterns are biocompatible and that the covalent crosslinking of our proteins can stabilize the gradient pattern for a very long time (up to several months). We believe that this method could be an ideal tool for studying long term effects of cell growth, migration, and differentiation on gradient protein patterns in either 2D or 3D.
We have developed a tool that is highly efficient in making user-defined spatial 2D and 3D gradient protein patterns. We achieve this by combining recent advances in synthesizing intrinsically photoactive proteins with a projection lithography system that employs a Digital Micromirror Device (DMD) as a dynamic mask. The simplicity of our technique makes it highly amenable to high throughput applications requiring such gradients.
The system that we report in this paper is highly flexible. While we have demonstrated the viability of our system using a few specific user-defined complex shapes, we believe that the dynamic mask being utilized here makes reconfiguration to any desired gradient profile quite easy. The efficiency and ease of use makes it particular useful for complex shape and gradient profile patterning compared to currently available techniques. One prevailing technique to create gradient patterns uses integrated microfluidic channels with diffusion profiles. Gradient profiles created by this technique are limited by the diffusion process—therefore, redesign and reconfiguration of such profiles can be complicated.
The properties of the pattern obtained using our methods are very suitable for high throughput biological studies. Pattern size and resolution can be varied by delivering light through different reduction rate lenses. Such flexibility in pattern size and resolution provides the opportunity for studies ranging from subcellular to tissue-length scales. For example, a millimeter-sized pattern yields submicron resolution suitable for subcellular microenvironment control (Takayama et al. 2001). In contrast, patterned areas up to centimeters can be easily produced for studying cell interactions, migration, and organization within groups of cells. As proof of the utility of this technique, we demonstrate that the aECM-N3 patterned substrates are biocompatible. PC-12 cells grown on these substrates demonstrate typical neuronal morphologies with neuritic processes observed to exhibit path-finding modulated by 3D gradient topography.
The fabricated patterns are very stable due to covalent crosslinks between the protein polymers and the aminated glass substrate. The mechanical properties of the aECM can be tuned by tailoring the concentration of N3 incorporated, the extent of crosslinking, and the weight percentage of the protein solution. In addition, due to the templated synthesis of aECM engineered proteins, the scaffolds can be easily tailored by including multiple peptides with distinct functionalities for specific cell and tissue applications (Carrico et al. 2007; Straley and Heilshorn 2009). Additionally, this method can be extended to create patterns of multiple proteins by following the initial patterning step with subsequent patterning steps. These types of complicated gradient patterns have been inaccessible to experimentalists to date due to technical barriers.
The capability of fabricating complex 3D microstructures makes this technique ideal for the creation of microenvironments with highly tailored chemical, mechanical, and topographical patterning. The ability to easily create these patterns across a range of length scale – from subcellular to tissue-level – makes this technique ideal for studying cell-environment and cell-cell interactions. Thus we have developed a method that can design and fabricate a wide variety of 2D and 3D engineered in vitro cellular environments for future research in developmental biology, regenerative medicine, and tissue engineering.
Phenylalanyl-auxotrophic Escherichia coli harboring the aECM plasmid and mutant PheRS gene was a kind gift from David Tirrell, Caltech. S. W., A. W. and X. Z. acknowledge financial support from NIH Nanomedicine Development Center (Center for Cell Control, PN2 EY018228) and NSF Nano-scale Science and Engineering Center (NSEC, DMI-0327077).
Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Sarah C. Heilshorn, Email: ude.drofnats@nrohslieh.
Xiang Zhang, Email: ude.yelekreb@gnaix.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Children's Colorado in Colorado Springs is now home to 30-plus pediatric specialists, ranging from orthopedics, neurology, audiology, endocrinology, and much more. Simply put, your neighbors are the specialists you'll find across all three of our locations here in town. Whether you see us at Briargate or Printers Park, our experts will be ready to partner with you and your child's doctor to keep your child healthy, safe and well. After all, that's what living in the Springs is all about.


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Elizabeth Gabriel has been a volunteer for Wild Care Cape Cod for over twenty years, a wildlife rescue and rehabilitation center for the lower Cape. Elizabeth also formed and serves on the board of the Provincetown Animal Welfare Committee.
Although it is impossible to leave no footprint, we want tread softly on our fragile environment whenever possible. At our Provincetown green hotel, we believe it is not just a decision, it is our moral responsibility.
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•January 1, 1970
Celebrity, television, film and music photos
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Zavarov: ...It was never Sanders's convention to begin with. The sole consolation for this hypocrite is the demise of Wasserman-Schultz, compliments of WikiLeaks's Julian Assange. If Sanders believes he's made an impact on Clinton's agenda, he's only deluding himself. Like Bernie in the film Weekend At Bernie's, Bernie Sanders is a walking dead. And like a worm being swallowed by a bird, he has lost his identity and has become a political protein incorporated now in what was once his enemy. This, to the disappointment of millions of his former followers.....


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Haha it's funny when you have MMA guys the know the deal on boxing! FUUUUU.CK YOU ARUM!
Thanks White!
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**** dana white hes a f**got
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Larry Merchant is the type of guy that will criticize you with a smile on his face to make it seem like he is just joking but he is dead serious.
I can't stand that old ****. That being said, what he said to Floyd was badass and he gets respect from me.
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I don't like Merchant but White is acting as if Merchant was criticizing Mayweather for the win which is bull****. Merchant was the only one on the HBO team who seemed content with the ending, I think he really enjoyed the fight.
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This tremendous colony ship is the largest ship of the Kel'Regar, grown and built in stages, birthed within the great 'dry' dock of Regar-kel-Osh. Fully organic, this ship is the ultimate expression of the Kel'Regar's power.
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U.S. voters also elected $10.9 billion in local-government debt Tuesday
Our nation's next president wasn't the only thing elected last night.
So was $10.9 billion in local-government debt.
Voters in the U.S. passed at least $10.9 billion in local government bond issuances, according to Bloomberg. There was $37 billion in borrowings up for vote yesterday, which is 45-percent less than in 2008.
"There is a reluctance to commit to new ongoing spending given the uncertainty of revenue," Tracy Gordon, a state and local-finance economist with The Brookings Institution, told Bloomberg. "Similarly, there is a hesitancy on the part of voters to incur debt when they are still recovering from the recession."
Some of the projects approved by voters include $642 million in funding for sewer improvements in Dallas and $1.3 billion in highway construction in Arkansas, according to Bloomberg.
About 63 percent of the $307 billion in debt issued since Oct. 25 has been to refund higher-cost debt.
"That trend is going to continue as voters across the country continue to feel the need to keep debt at a low level," John Loffredo, co-head of MacKay Municipal Managers in Princeton, New Jersey, told Bloomberg.
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text: Raekwon Reacts to him being #10 on Hottest MC list By admin | October 1, 2009 [COMMENTS] Raekwon Reacts to him being #10 on Hottest MC list Share this video Tweet Related Videos D.R.A.M. feat. Lil Yachty "Broccoli" Video Gucci Mane "Out Do Ya" Video Gucci Mane "Maybach" Video Joe Budden Speaks On Why He Dissed Drake – The Brilliant Idiots Birdman "Breathe" Video News & Songs › ‹ Ramriddlz – H2O Lil Wayne Walks Off Stage During High Times Concert (Video) Soulja Boy – Rockstar Vado Feat. Jeremih & Cam'ron – We Da Wave Gucci Mane Brings Drake Out At Homecoming Concert (Video) Remy Ma – My Shit (Remix) N.O.R.E. Feat. Memphis Bleek & Remy Ma – 4 Da Win Joe Budden – Just Because DJ Khaled Feat. Kendrick Lamar, Big Sean & Betty Wright – Holy Key Wale Has A Baby; Cancels New York Concert Drake Fined For Performing Too Long At Dallas Concert? ASAP Rocky & Kendall Jenner Dating?


text:
British Firm Tries Out Virtual Receptionist
Shanice is about to start her job as the receptionist at a new local government office in London. She also happens to be a hologram. Officials say that at a cost of $19,000, she's much cheaper than a living and salaried alternative.
And our last word in business today is more of an introduction: Welcome to Shanice, who is about to start her job as the receptionist at a new local government office in London. She also happens to be a hologram.
That's right, a hologram receptionist. Officials say that at a cost of $19,000, she is much cheaper than a living and salaried alternative. But she can only respond to a small number of questions - like directions to different offices, or maybe how to use the elevator.
SHANICE: To use the lift, please call it by holding the button and keeping it held until the lift arrives.
MONTAGNE: And hopefully, she also knows the answer to the question receptionists get most often: Where's the bathroom?
MONTAGNE: And that's the business news on MORNING EDITION, from NPR News. I'm Renee Montagne.
GREENE: And I'm David Greene.
Copyright © 2013 NPR. All rights reserved. Visit our website terms of use and permissions pages at www.npr.org for further information.
NPR transcripts are created on a rush deadline by Verb8tm, Inc., an NPR contractor, and produced using a proprietary transcription process developed with NPR. This text may not be in its final form and may be updated or revised in the future. Accuracy and availability may vary. The authoritative record of NPR's programming is the audio record.
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Press L, Z, R, C-Up, C-Down, C-Left, C-Right, Start at the main menu. A sound will confirm correct entry. All racers, the Joker and X cups, and the Master difficulty setting will be unlocked.
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text: At G2 Korean BBQ we welcome you to come and enjoy your next dine out at a modern fusion traditional Korean BBQ meal.At G2 we strive to provide you with traditional Korean dishes and at times adding a twist creating a modern fusion taste. We accommodate for all types of customers and groups. We are open 7 days a week and trade for lunch, dinner, and night everyday till 4am. So, for your next night out, birthday dine or a corporate function be sure to contact G2 Korean BBQ and experience quality Korean dining at the heart of Melbourne CBD.


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I soooo want this shirt. I can imagine me wearing it the next time I visit with my friend who so loved The Motorcycle Diaries. But, really ... it could be worn anywhere ... college campuses, liberal bookstores. The possibilities are endless.
Have we reached Peak Depravity yet?
13 hours ago


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In Straight Talk with Your Kids About Sex, Josh and Dottie McDowell draw on their decades-long love and concern for youth. Building on the Why True Love Waits campaign and utilizing up-to-the-minute research from Josh's The Bare Facts resources, the McDowells provide a compact, easy-to-use guide to support parents in
- putting sex and sexuality in a biblical context of relationship to God
- gauging their child's emotional and spiritual readiness
- being alert to questions and opportunities
- being direct and open without violating their child's emotions
- proactively shaping their child's worldview in today's sex-saturated culture
Working from their own experience with four children, Josh and Dottie give readers encouragement and solid information in the sometimes-awkward process of guiding their child into a healthy understanding of God's gift of sex.
Product Reviews
IreneUnited States5 Stars Out Of 5January 20, 2013IreneUnited StatesQuality: 5Value: 5Meets Expectations: 5This review was written for Straight Talk with Your Kids About Sex.This book is a must read for parents! This is the best book I have read on this subject. It is easy to read and filled with helpful tips on having a great relationship with your child so any talk you have with them is beneficial.
I gave this book 5/5 stars. As I read this book I could tell that the authors really have a heart for helping families grow together. I liked how they approached this subject and wrote about it with honesty. The tips were helpful and easy to implement in your conversations with your children. I recommend this book to all parents no matter the age of your children. You will find this book helpful and it is well worth your time in reading!
I would like to thank NetGalley and the publisher for the copy of this book I enjoyed reading. I gave an honest review based on my opinion of what I read.
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text:
About Dick's Last Resort - Las Vegas
Dick's Last Resort is known for its outrageous, surly, and energetic servers who dish out good grub, cold booze, and heaping helpings of sarcasm. From rug-rats to old farts, from high-class to no-class, from the top of the food chain to the bottom of the barrel, Dick's is fun for all and all in fun for those who dare to enter. The servers are always fun, but if yer unlucky enough, you may even catch a little live music. There's never a dull moment in this 3-ring circus which is exactly how Dick's earned its infamous reputation!
WARNING: You'll have a good time whether you want to or not.
Dining Style:
Casual Dining
Barbecue, American
Hours of Operation:
Breakfast: Daily: 11:00am - 4:00pm
Lunch: Daily: 11:00am - 4:00pm
Dinner: Sunday - Thursday: 4:00pm - 11:00pm, Friday - Saturday: 4:00pm - 12:00am
Cross Street:
Dress Code:
Casual Dress
Price Range:
$30 and under
Payment Options
AMEX, Discover, MasterCard, Visa
Phone Number:
(702) 597-7991
Come see our live band every Saturday night from 11pm to 1am. Put on your dancin" shoes while you grub or drank! Watch our infamous "Taco", give lap dances and spin cool jams. Sing-a-long for more fun.
Executive Chef:
Michelle White
Private Party Facilities:
Our patio is available for up to 250 guests. Full bar available as well as private entrance. Semi private dining options available inside the restaurant.
Private Party Contact:
Emily Hedlund: 7025977991x
Banquet/Private Rooms, Bar Dining, Bar/Lounge, Beer, Counter Seating, Dancing, Entertainment, Full Bar, Gluten-free Menu, Happy Hour, Late Night, Non-Smoking Area, Non-Smoking Restaurant, Patio/Outdoor Dining, Private Room, Smoking Area, Smoking Area (patio only), Takeout, View, Wheelchair Access
Send a Gift Card
Buy now and send it instantly by email, or print a card to give in person.
Dick's Last Resort - Las Vegas Ratings and Reviews
This was my 1st time at a Dicks Last Res ...
This was my 1st time at a Dicks Last Resort and we had a great time! Our party of 10 ranged from 21 years old to 77 years old, I was pleasantly surprised everyone love the experience equally! I wish we had a Dicks closer to where we live, we'd definitely go back!
This was absolutely amazing. I had no i ...
This was absolutely amazing. I had no idea the experience I was about to embark on. The wait staff, balloon guy and everyone around us went above and beyond to make this a fabulous birthday experience for my family. I highly recommend it to anyone with a open, fun filled mind wanting a great laugh and good fun. Thanks to all!
Food was boring and way over priced. Se ...
Food was boring and way over priced. Seating options are shared picnic tables with other diners or the bar. Way over priced even for vegas. Servers try to force the fun and some are entertaining. quick with the food and drink refills.
Was overpriced for the type of food offe ...
Was overpriced for the type of food offered. Even that wouldn't have been too bad except the food wasn't even that great to recommend this restaurant. Different kind of experience due to the service but my husband, children & I all said we would not go back.
terrible service... ironically due to th ...
terrible service... ironically due to them not having a joke with you! we were a bachelor party and the groom dressed as a large appendage so we expected plenty of abuse but the waitress was not impressed and gave no banter out and when someone told her to cheer up she asked us to leave! terrible
Our waiter was fantastic. My wife and i ...
Our waiter was fantastic. My wife and i had been before and sat at the bar, so when we new we were going back with a group of six people we made a booking to show our friends. Everyone loved it and the food was great too.
Fun times, food not so great
We came to dinner here on of our nights in Vegas with a group of girls. We made a reservation but didn't need one because there was plenty of seating. The service was fantastic! We enjoyed our experience very much. However the food was not spectacular. One friend has appetizers, two of us has the fish n chips and another has the salmon. As a person who is particular with their food I didn't like that the menu offered no descriptions and the overall quality of the food was no good. The fish n chips was greasy, and mushy. I didn't eat much of the entree because it tasted so bad. On a better note our waitress was hilarious and we'll always remember those hats.
Fun but expensive for Ok food
We took our two sons and a friend (5 of us total). We enjoyed ourselves as the staff was Rude (per expected, in a good way), we laughed and enjoyed ourselves and watched everyone else and what was going on around us. The prices were ok but compared to the flavor of the food that we received it was a bit disappointing and the prices of the drinks aren't even displayed so you have no idea how much until you get the bill. Yes we had fun but for $200 we could have eaten at other restaurants and had a wonderful meal and drinks for less other than a fancy steakhouse them we would have spent about the same or a little more due to steaks & seafood. I had the ribs that weren't bad, ad to either chew through and spit out or peel off of of the meat and bones before I could eat it. One ordered the salad and it was a good portion size but barely any salad dressing for the amount of lettuce.
Enjoyed our experience
We went to Dicks for my daughter's 21st birthday, and enjoyed the whole ambience.
We ordered drinks in souvenir glasses, and they were pricey but ample. We had the
Dolly chicken breasts which were average, but filling. The best part was our server,
who was "rude" but not mean. We would definitely go again.
Subpar food and overpriced, it's Vegas we expect to pay for food but probably our least favorite in 6 trips. Our waitress was fine but seemed more interested in visiting coworkers than our table. The big thing at Dicks is the paper hats only 3 out of 6 received a hat. We will not return and will not give a positive review to any of our friends.
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text:
COCK A HOOP | The Sydney Roosters re-entered the winners circle last night, while the Broncos' form ...
15 hours ago - 2 Likes
The Bradford Bulls have confirmed the dates of a series of special events to celebrate their Centenary season in style.
1907 saw the start of the Bradford Northern Club following the decision of the original Bradford Club to switch to Association Football. The current Bradford Bulls are proud to trace their history back to this momentous year.
Bulls Chairman Peter Hood said today:


text:
Google's Sandbox Documented?
If you're into Search Engine Optimization you might be interested in checking out this article and the comments at this Threadwatch post titled Google's War on SEO. It refers to a recent patent by Google that could shed some light on where they're going with their algorithm. It is pretty ...moreSearch Engine Optimization
Google SEO Tips
If you want to spend some time this week optimizing your blog for Google then you might want to check out some of these articles on SEO for Google that came up in my RSS feeds this morning. They are good solid tips for people wanting to learn some SEO ...moreSearch Engine Optimization
What does Google display under your page title?
Antone over at SEO SEO Secrets of the PageRank Masters has finally updated his blog with a post on determining what Google displays under your page title in search results which a few people have asked me about recently. I hope it helps. 'So how do you control what text ...moreSearch Engine Optimization
Frequently Asked Questions on 'Links'
Wayne over at SEO Chat has a good article on Links – Links: Frequently Asked Questions. It might be of some use to those of you interested in optimizing your blogs for Search Engines. Don't be put off by the basics at the start (ie first question is – 'what ...moreSearch Engine Optimization
Formatting images for SEO
One of the most commonly known Search Engine Optimization (SEO) tips that go around has to do with the way you name and tag the images that you use on your site. Its fairly commonly accepted by most SEO experts that Google not only looks at the text on your ...moreSearch Engine Optimization
Increase Traffic to Your Blog from Search Engines – The Top 5 Tips
Tinu Abayomi-Paul post an article over at Search Engine Guide on how to Increase Traffic to Your Blog from Search Engines – The Top 5 Tips. Might be useful for some – although nothing really new here – you'll find it all in my SEO archives too.Search Engine Optimization
Getting Yahoo Traffic for your Blog
One of the constant frustrations that I've had with blogging over the years has been my inability to rank well in Yahoo's search engine results. Despite the fact that Google seems to love some of my blogs – Yahoo has always been rather standoffish with them. It does seem to ...moreSearch Engine Optimization
Search Engine Optimization for MSN Search
One of the nice surprises that we've had over at the Breaking News Blog collective over the past few months has been the way that MSN Search has ranked our blogs highly. Even though the blogs are new and are yet to have the many links that their competitors have ...moreSearch Engine Optimization
Google Back Link Update in Progress
Google seems to be doing a back link update at present. I've noticed significant changes in some of my blogs back links so far. Traffic changes and SERPs often follow such updates. Update: Others are seeing it too Update II : Sorry for my lack of explanation here – I've ...moreSearch Engine Optimization


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Experience the Valley's best and most celebrated Southwest holiday tradition as the magic of thousands of hand-lit luminarias light the pathways of the Garden. Enjoy the sounds of talented musicians throughout the Garden trails, and make plans to dine with family and friends. This year, Las Noches de las Luminarias is offered for 20 nights and will include the exhibition of Bruce Munro: Sonoran Light at Desert Botanical Garden.
Las Noches de las Luminarias Schedule:
November 27-28, 2015
Decemeber 10-13, 16-23, 26-31, 2015
To purchase tickets order online at www.dbg.org or call 480-481-8188
"America's Original ArtWalk" The 40-year tradition of Scottsdale ArtWalk takes place every Thursday evening from 7 p.m. – 9 p.m. in galleries along Main Street and Marshall Way in downtown Scottsdale. Once a week, the galleries extend…More information »
Celebrate Native American culture with weekly performances of dancers from around Arizona sharing the traditions of their tribes. The performance includes hoop dancing, known as the "dance of life." Performances take place at the Fountain Court at the Hyatt Regency Scottsdale Resort…More information »
Arizona is center stage for the 2016 College Football Playoff National Championship on January 11, 2016. It's a new era of a four-team playoff, with two semifinals played in bowl games and a championship game played in…More information »


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Possible Answer
Adultery can be used as a basis for divorce in North Carolina. In addition, North Carolina is one of the... - read more
Adultery in North Carolina: Does Cheating Affect Alimony? ... Under North Carolina criminal law, adultery... - read more
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