text:
Not to spring a spoiler on anyone, but halfway through the movie there's an intermission where Anna Kendrick performs a ten-minute solo softcore scene.
Why review a flick that's not playing in Charleston?
Ponijao from Namibia was actually a girl! The scene where she was practicing "carrying" on her head showcases this. In this culture, that is not something that a male child would do.
This movie was awesome! Actors were awesome and the writer of this article was NOT awesome. Shame on you for putting a negative report on this movie.
I enjoyed it, and as a lifelong Marvel fan, I appreciated that it remained mostly true to the original comic.
My, don't you have a rarified sense of humor.
So saw this last night.. not terrifying at all. Pretty disappointed actually. They had the right ingredients but it just came out wrong.
Sounds like I'll wait to see this one. I will say that I agree Pulp Fiction is his best work, but people tend to overreact to a stinker. I personally really enjoyed Inglorious Basterds, especially the performance of Christoph Waltz. It's probably my second favorite Tarantino. Part of his genius is that he's always introducing me to new and interesting people. Selma Hayak blew my doors off. There's also Steve Buscemi and Tim Roth whom he introduced me to. Tarantino reinvented Harvey Keitel and John Travolta. Christopher Walken was great with Dennis Hopper in True Romance before he did Pulp Fiction. I love his taste in actors, excepting Uma Thurman, of course.
Agreeing with Mat & Prong, Pulp Fiction was the Greatest Tarantino. As for this one its the same old same old same old. I started getting antsy 25 minutes into the movie having shoveled in most of the popcorn so I got up and went to the snack bar for a box of year old duds and a coke so I could at least feel wide awake and sick instead of just bored, killed 15 minutes that way. Using the not feeling good ploy allowed me to escape the movie for another 25 minutes a short while later. I just wandered around the lobby. It was worth the "Why do you eat that crap, you always do this lecture" to not to have to sit through that movie. All that talent wasted and lets be honest....it was boring, predictable and really overrated. Any movie that I start thinking about how my ass hurts from sitting and my adult ADD starts boiling is not good. On a scale of 1 to 10 Ass Hurts and ADD rating, this was a 8. Who wants to see The Revenant? Alejandro González Iñárritu and DiCaprio. Now that looks like a movie that I wont start thinking about my ass and what kind of snack I should get next.
Tarantino is a jerk and I will not knowingly see anything he has done.
^ Agreed, this movie was excellent and a crash course for many in what happened and what will happen again. Moody's and S & P are still a joke, who believes anything those whores say? Certainly no smart investor takes them seriously. The sad thing is they were still settling with fines for lying in 2015. No jail time for big dollar crooks, the DoJ lets them buy their way out instead of prosecuting those responsible. We all know if it happened on a smaller scale by a regular business, heads would roll and somebody would do some time. S & P paid 1.5 billion in settlement in 2015 as well as an additional almost $80 million to state and federal authorities over claims it misled investors in 2011 (That's right folks 2011 - right back to bullshitting after the 2008 fiasco) about ratings of commercial-mortgage backed securities in January 2015. So in other words, NO, you absolutely, positively can not and should not ever believe the Bullshit Bond Ratings. The ratings are fueled by 2 of the continuous proven - heavily fined bullshitters ever. Did you know that S & P accepted a DoJ condition and were not allowed to give ratings over this past year in part of the commercial-mortgage backed securities as punishment? They kept that on the down low and out of the press as much as possible. This is a a situation where both money and bullshit talks and neither walks.
Unfortunately, Tarantino does not make very good movies. This was not a good movie at all. Not even entertaining.. I found it boring too many times as it progressed and actually progressed is the wrong word - boring too many times as the time went by....
One of the best movies I saw in 2015. Carell will be nominated for an Oscar I predict. Gosling is a real a-hole in this one and steals the screen when he's on. Bale is nothing short of amazing also as (probably) on the autism spectrum. He also will be nominated. The film is funny but FULL of anger- the director's and Baum's, played by Carell.
Here's the thing- has it truly stopped, this greed and corruption? In the movie they make it clear that BOTH Moody's and S&P overrated bonds by a wide margin due to greed, fees. Ergo, you can't believe the bond ratings, probably even today. This film will scare the living sh*t out of you. Go see asap.
People that think he's the best filmmaker going are typically the kind of meatheads who don't ever read.
^ Bang on.
Peaked with "Pulp Fiction" (1994) (masterpiece). Made one more great film ("Jackie Brown").
Parts of the "Kill Bills" were entertaining, but both a country mile from being great films.
The case could be made that he's become the most overrated filmmaker of this generation.
Tarantino was never what most folks made him out to be. Sure, he was a talented filmmaker, in exactly the same way all those folks who crank out near-perfect reproductions of famous paintings are talented painters.
So, if he's only left to mimic himself now, that seems just about perfect.
Meanwhile, five days later... VICE agrees: http://motherboard.vice.com/read/the-force…
Thoughts the movie was very good. Lots of comic relief, good action sequences, new characters to continue the franchise. Not let down at all.
No click bait, this is the most honest review out there. I just finished seeing it, and it was just plain awful. Nothing more than reprocessed cheese.


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A new benchmark: performance monitoring without the WM Charity Index
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Stephen Orr
Seeing Trees: Discover the Extraordinary Secrets of Everyday Treesby Nancy Ross Hugo
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Have you ever seen the delicate flowers of a red maple? The emerging leaves of a tulip poplar? The twigs of a beech? When you look at a tree up close, you begin to appreciate trees in a whole new way. Seeing Trees invites readers to watch trees with the care and sensitivity that birdwatchers watch birds. Focusing on 10 common trees of North America, Nancy/i>
Have you ever seen the delicate flowers of a red maple? The emerging leaves of a tulip poplar? The twigs of a beech? When you look at a tree up close, you begin to appreciate trees in a whole new way. Seeing Trees invites readers to watch trees with the care and sensitivity that birdwatchers watch birds. Focusing on 10 common trees of North America, Nancy Ross Hugo highlights the rewards of tree viewing and describes some of the most visually interesting leaves, flowers, fruits, buds, leaf scars, twigs, and bark of familiar trees. Using software developed for work with microscopes, Robert Llewellyn created incredibly sharp close-up photographs of the tree detail by stitching together 8 to 45 images of each subject—each shot at a different focal point. The combination of these lavish photos with Nancy Ross Hugo's writing makes each page come alive with the beauty of the growing process. The result is a gorgeous journey into the life cycle of trees.
Featured trees include the American Beech, Ginkgo, Red Maple, Southern Magnolia, Tulip Poplar, White Oak, White Pine, American Sycamore, Black Walnut and Eastern Red Cedar.
Editorial Reviews
Stephen Orr
Mary Beth Breckenridge
"A whole new world of tree mystery has opened up."
"If you love trees, or if you love good photography, you will love this book."
"A new exciting book."
"Gorgeous images & observations."
"You will begin to appreciate trees in a whole new way."
"This fascinating celebration of trees will delight gardeners, botanists, students of natural history, and nature photographers."
"The book to change us all into unabashed tree worshippers."
"The only way I can describe my reaction to receiving Seeing Trees was like a child being taken into a sweet shop."
"A beautiful and exciting book."
"A splendid book."
"Hugo writes with real passion about trees…. Beautifully produced and fascinating to read."
"A botanical masterpiece."
"Filled with surprises."
"Their call to seeing what nature offers is magical and the photographs are works of art."
"This seems like the sort of book which sprinkles a bit of fairy dust on something we see everyday, so that just taking a walk or stepping outside feels magical and fresh."
"If you love books and nature, this is one to own for reference and to ponder during the long winter months."
"A captivating book."
"A gorgeous book, a great reference and a beautiful addition to the nature lover's bookshelf."
"Will take your breath away."
"My favorite new book this season is Seeing Trees…This book is made for us nearsighted gardeners, who long ago learned the thrill of peering at plants."
"You can't help but be bowled over by the beauty at play in the science."
"The authors have brought the level of observation to new heights."
"Through [Llewellyn's] lens we take flight with a red maple's charming helicopterlike seed pods and can almost feel the smooth, muscular, steel-gray bark of an ironwood."
"Llewellyn's extraordinarily crisp photographs alone force the reader to consider trees differently, if only because there are so few illustrations of entire trees, trunk, crown and all."
A beautifully produced and photographed new book. I highly recommend this book to anyone who would like to know more about this fascinating group of plants both in the wild or in your backyard.
Filled with arresting close-ups.
You'll be dazzled.
Seeing Trees opens our eyes to a tree's shy magnificence and invites us to deepen our relationship with these earthly treasures.
Seeing Trees opens our eyes to a tree's shy magnificence and invites us to deepen our relationship with these earthly treasures.
"Vivid, fascinating botanical biographies."
"The resulting images are full of detail."
"An idiosyncratic portrait of common trees and their life span, Hugo's book introduces aspects of tree culture that delight and bemuse."
The New York Times Book Review
Product Details
- 9781604692198
- Publisher:
- Timber Press, Incorporated
- Publication date:
- 08/16/2011
- Pages:
- 245
- Sales rank:
- 131,502
- Product dimensions:
- 8.50(w) x 10.10(h) x 1.00(d)
Meet the Author
Nancy Ross Hugo has been combining her love of the outdoors with her love of the written word for more than thirty years, as garden columnist for the Richmond Times-Dispatch, education manager at the Lewis Ginter Botanical Garden, and writer for Virginia Wildlife, Horticulture, and American Forests, among other publications. She is co-author, with Jeff Kirwan, of Remarkable Trees of Virginia (2008) and, in collaboration with photographer Robert Llewellyn, of Seeing Trees (2011), upon which this book is based. Nancy and her husband, John, divide their time between Howardsville and Ashland, Virginia. Visit her at nancyrosshugo.com.
Robert Llewellyn has been photographing plants and landscapes for more than forty years. His photographs have been featured in major art exhibits, and more than thirty books featuring his photography are in print. His book Washington, The Capital was an official diplomatic gift of the White House and State Department. Llewellyn's previous books, Seeing Trees and Seeing Flowers have both earned glowing reviews; Seeing Trees was chosen as one of the best gardening books of the year by the New York Times. To learn more about Robert Llewellyn and his work, visit www.robertllewellyn.com.
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GLPVC Presents James Olson - Merging the Hemispheres of your Brain for Genius - 11/12 @ 11pm est
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Very interesting topic. I have been in search of what can open up the Pineal Gland "seat of the soul" or "gateway to enlightenment" – Our Third Eye. De-calcifying and activating the gland after years of being poisoned by fluoridated water and chemical laced foods.
If this is truly the "seat of the soul" or the "gateway to enlightenment" then humanity has successfully scuttled this natural path to self-knowledge. The environment we are in right now, sitting in front of our computers, is having a negative effect on our pineal gland. So I ask the question...is there anything we can do to improve the health of this important brain structure to be able to merge both hemispheres of our brain?
Thank you!!
Wake Up
Just did a search on this site (
Adv. Search
link located top right side of page)and there are over 50 thread on the pineal gland within the past 2 years. I'm going to go back and read them tonight, most referenced things I have not read about before in relation to pineal gland. Looks really interesting
Ralph--a house dog
Thanks Ralph...I will check some out when I have more time.
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13 Important Elements To Look For In Every Staged Shooting Event
Gestapo In The USA: FCC Intimidates Press and Kills Free Speech at 5G Rollout
Democratic National Convention in Philly Is Not What We're Told About in the Media
Federal Judge Rules Hidden Microphones in Public Do Not Violate Constitution
Deutsche Bank's net income plummets
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Black Protesters at DNC SHOUTS: "Don't Vote For Hillary, She's Killing Black People"
Hillary Clinton will nominate justices to the Supreme Court who are prepared to overturn Citizens United."
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Superhuman Tech? Most Fear Worst..
Wikileaks Proves Primary Was Rigged: DNC Undermined Democracy
WIKILEAKS JULIAN ASSANGE Says He Has NEW Emails That Should Indict Hillary…And Why He's A Trump Fan
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DNC erects four-mile eight-foot tall fence around Philly convention site
Should the Postal Service Handle Your Loans as Well as Your Mail? Elizabeth Warren Thinks So.
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The Press has reluctantly given Cecile Meier leave to visit France and get married. In her absence, some of the people mentioned in her column get a right of reply. Today it's her fiance's turn.
I've been villainised in this column for almost a year. I've become known as the guy who got Cecile lost in the mountains, who made her ride narrow trails on the Port Hills by saying they were easy, and who asked her to bring him a beer while she was sworn off alcohol for a month.
Now I have a chance to clear my name and dish some dirt in return. I could tell you about the "Pensky Incident", what she does with her slippers in the morning, or what she thought a shed was. But I'm not going to.
Mostly that's because of how big-hearted I am. But also because it's not long until the wedding and she'll read this beforehand.
It's not that Cecile makes stuff up, it's just that she doesn't understand what some words mean in Kiwi - mostly outdoors-related words.
Whenever we go tramping, Cecile insists I find "easy" tracks. The problem is that "easy" and "sweeping vistas with alpine streams" don't always go together and I really like alpine streams. I don't ignore her request, I just tend to interpret "easy" in the way I was taught by various Barry Crump books. Easy was anything that didn't kill you.
Next thing we're slogging up some easy "track" using tree roots for handholds and somehow I'm the bad guy. They don't have Barry Crump in France.
Cecile got very upset once because we were lost. This was a challenge for me because I didn't think we were lost at all. To me, you really need to be tired, cold, hungry, out of food, with no tent or sleeping bag, and not know where you are to be "lost". That wasn't us - we just happened not to be on the track, which meant being creative in our route choices. This explanation fell on deaf ears - Cecile was confident we were going to die. Thankfully, that was when I worked out where the track was.
It's not all a one-way street though - I'm learning too. Example: how to react to being cold. I grew up thinking cold was just one of those things you got sometimes. You put on a jacket maybe and if that didn't help you popped a couple of harden-up pills and got over it. Cecile doesn't share this view.
Her attitude to being cold is a bit like my attitude to totalitarian regimes. Something must be done! The world must act!
On this, I'm coming around. Stoic endurance of cold when there's no option is hardcore, worthy of bragging. Stoic endurance of cold when there are perfectly viable alternatives though, that's misguided. I still swim in mountain tarns, but I wear all the hats and down jackets afterwards.
We've warped each other I guess. Seems like a good foundation for marriage.
In closing: I wasn't lost and the trail was easy. On the beer charge, I plead not guilty on grounds of insanity through thirst.
- The Press
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Reflection lost in the glare of screens
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Some cities are just far too tempting
Statistics almost put me off biking in Christchurch
Cecile: How to avoid the blame game
Week in review: Where the women are tough and the rats are scared
Editorial: The Government has to get the new agency for children right this time
Building blocks in place for Chch to be a city with opportunity
Editorial: ECan's promise to get tough on people stealing water needs monitoring
Publicly-funded surgery granted days after Doug Pike sold his house to pay for surgery
Neighbour heard man scream for help while Christchurch flat burnt down
Kindergarten teacher saves child from abuse
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Jordie Barrett a star on the rise in Canterbury rugby
Former Crusader lock to miss Canterbury plate final due to mouthguard gaffe
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Super Rugby showdown looms as All Blacks trial for halfbacks Perenara and Kerr-Barlow
Senior Sergeant Richard Ryan farewelled by 500-strong guard of honour
All Whites great Steve Sumner urges men to 'get tested' as his own cancer spreads


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I am planning to renew EAD/AP for me and my family. I have applied my employment based I-485 in Jan 2012. We received EAD/AP combo cards in Mar 2012. My family (wife and kid) had traveled to India for 2 months. They have used their AP at port of entry. I'm still maintaining my H1B.
1. Do I need to pay any renewal fees? I am guessing since I applied after 30 July 2007, I don't have to pay any fees.
2. Form I-765
a. Column 13: Manner of last Entry. Is it Paroled or Parolee or Advanced Parole?
b. Column 15: Current Immigration Status . H4/AOS Pending
c. Column: 16 (C) (9)?
3. Form I-131 Column: 3 Class of Admission. ?
4. Which USCIS address should I send? My service center is Nebraska.
5. Do I need to send all EAD/APs together or in different envelops?
Thanks in advance,
Immigration Attorney
1. Any time your applying for benefits from USCIS they will make you pay application fees unless you get a waiver and those are hard to obtain and I would not recommend you applying for one it could jeopardize your I-485 application.
You should hire competent counsel to assist you with the rest of the application specific question. Call us or any of the other colleagues.
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IndyGo donating summer bus passes to Project Indy
The partnership was announced Wednesday.
The partnership was announced Wednesday.
Talk about fun for both kiddos and parents! Today on Indy Style, Amber and Nina "try out" some kid-friendly products that you're sure to lov…
The victim originally said Miley molested her in May and June of last year but has since said no criminal act was committed.
A Hobart man jumped off a Lake County bridge late Tuesday night after crashing his car on Interstate 65.
Being a homeowner definitely comes with its share of projects and repairs. When it comes to your home's roof though, how do you know when it…
The Consumer Product Safety Commission said Wednesday that it has received 99 reports.
Owners who lost their pets can walk through the shelter or visit Animal Care and Control's website.
The homes were raided Wednesday.
Any true bicyclist knows…. there is a right way and a WRONG way to ride. Brian Gootee, of Gray Goat Sports, explains how we should "mind our…
Akram Musleh had faced a July 11 detention hearing in Indianapolis.


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A Covington man was shot in the hip on May 28 following an end-of-the-year party on Stone Road, according to the Newton County Sheriff's ...
Long-time Oxford city council member Terry Smith resigned from the council, Mayor Jerry Roseberry announced today.
Richard Shaw and his band Creation will appear at Local Grown Jazz by the River, a fund raiser for the Washington Street Community Center in ...
The Porterdale Police Department and Porterdale Citizen's Police Academy Alumni will hold block parties throughout the village on Saturdays through July 23.
The Georgia Department of Transportation (GDOT) announced its schedule for construction this summer and beyond. While there are four projects on the GDOT list, only ...
A College Park police officer was killed in a single vehicle crash Memorial Day on Interstate 20, according to the Newton County Sheriff's Office ...
It has been undeniable that a community needs water to survive and thrive.
The Newton County Board of Commissioners (BOC) heard from constitutional officers from the horseshoe at the Newton County Historic Courthouse Thursday on why their respective ...
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The longstanding land condemnation case between Newton County and Emmett Denby, the lone remaining resident to live on land planned for the future Bear Creek Reservoir, was decided this week in Superior Court when the county was ordered to pay Denby $800,000 for his property.
Spring break camp registration is going on at the Covington Family YMCA for children ages 5-12. The cost is $95 a week per child or $25 a day. The camp will run April 2-6. There is a $35 program membership fee if applicable. Financial assistance is available based on need.
A company wanting to build a private landfill in Newton County could yet get its way after the Georgia Supreme Court this week overturned a ruling that had effectively ended any chance of a landfill.
A Covington woman was arrested for the fourth time for shoplifting in Newton County Tuesday, this time at Kmart.
A store employee noticed Margaret Watkins, 26, take clothing and jewelry into the fitting room, then leave the store immediately after exiting the fitting room.
A call to a Bent Pine Court home early Sunday morning for stalking led to several arrests, including two for marijuana.
As the officers approached the front door of the residence they could immediately smell marijuana, and although they could see two males asleep on the couch through the blinds, officers knocked for about five minutes before anyone came to the front door.
The simple act of swallowing is something many of us take for granted. Yet every year, approximately 10 million Americans are diagnosed with swallowing disorders, known as dysphagia. Nearly all dementia patients develop dysphagia, and swallowing disorders are associated with stroke, progressive neurological disorders including multiple sclerosis and Parkinson's.
Two years ago, Cheryl East received news that would change her life and the lives of all who knew her. That's when she found out she had breast cancer. She's been fighting for her life ever since - a fight which hasn't been easy because of her rare blood type.
The brave actions of a victim, police and shoppers outside the Hobby Lobby on Ga. Highway 138 helped fend off an armed kidnapper and potentially saved the lives of a Conyers woman and her grandchild Wednesday afternoon.
The Social Circle City Council approved to temporary cutting water and sewer fees in half to spur home construction.
Interstate 20 eastbound was down to one lane around exit 88, because the state's Specialized Collision and Reconstruction Team was mapping the fatal crash scene from a pedestrian fatality, Georgia State Patrol spokesman Gordy Wright said in an email.
Roland L. Wilson was found guilty in the 2009 murder of 21-year-old William Okafor, who died as a result of injuries sustained from being struck in the head with a brick following an argument outside his home.
Police arrested Jamarcus Pridgett, the younger brother of convicted murderer Jamaal Pridgett, late Friday morning, after he reportedly tied up and assaulted his girlfriend.
A vehicle stop for a busted tail light turned into an arrest on drug charges early Sunday morning on Alcovy Road.
Faith Eller of Covington will participate in the 2012 Miss Jr. Teen Atlanta pageant Saturday. Eller submitted an application and took part in an interview session conducted by this year's Atlanta Pageant Coordinator.
The end of March is a busy time for the Newton County Judicial Center, with several trials, including those for murder, coming before the Superior Court judges.
Page 373 of 641


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Narrow your search
Planetary Resources, the new company for mining asteroids, has outlined its plans to pinch materials from space.
Planetary Resources co-founders Peter Diamandis and Eric Anderson discuss the space startup's goal to mine nearby asteroids for water, platinum, and other natural minerals for use on Earth.
A space startup says nearby asteroids can be mined for water, platinum, and other natural resources to enable space exploration and bring those valuable materials to Earth.
Marking the most successful Kickstarter campaign for a space project, Planetary Resources now has $1.5 million to launch its Arkyd telescope and bring asteroid, galaxy, and planet exploration to the public.
Asteroid mining company Planetary Resources has launched a $1 million Kickstarter campaign to raise money to put a publicly-accessible telescope into space, and fund educational research.
Planetary Resources seeks to mine asteroids to further space exploration and to bring resources back to Earth. Experts say the idea is no longer science fiction.


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This site hosts Web Audio samples which are referenced in my book "Web Audio API: Advanced Sound for Games and Interactive Apps". You can either read this book for free or, if you'd like to support the author, buy it from O'Reilly or Amazon.
- Web Audio API W3C specification
- Web Audio Examples
- Getting started with the Web Audio API
- Developing game audio with the Web Audio API
- Scheduling Web Audio with precision
- Web MIDI API W3C specification
- Web Audio playground
If you'd like to change something about this site (eg. add a link to your blog post, fix a sample, tweak the design), or have some other idea, please submit a pull request to the github repo. If you spot an error in the book, please contact me.
-- Boris Smus


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HTC Windows Phone 8X
The HTC Windows Phone 8X is one of the most attractive phones we've come across, but is not without its faults
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My first impression after unboxing the Q702 is that it is a nice looking unit. Styling is somewhat minimalist but very effective. The tablet part, once detached, has a nice weight, and no buttons or switches are located in awkward or intrusive positions.
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text:
VP North America at Neul, Luke D'Arcy, will present on the IoT technologies engineered by Neul with an emphasis on challenges and successes throughout their IoT initiatives.
You won't get better networking anywhere else! Hope to see you there!
6:30PM Networking (pizza & beverages)
6:50PM Brief announcements
7:00PM Luke D'Arcy presents on Neul
7:45PM Networking
Good information on a new wireless network approach...
0 · August 20, 2013
FYI: Neul was also a founder of the Weightless SIG for M2M over White-Space spectrum... more at: http://www.weightless.org
1 · July 30, 2013
Argh, it looks so cool. Can you do a live broadcast like google hangout? Or an audiocast.
0 · July 30, 2013
Meetup members, Log in
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text:
Eagle Group Steam Table Parts and Components
Outfit Your Steam Table with Sneeze Guards, Tray Slides, and More Using these Steam Table Parts and Components
Outfit your steam tables and cold food tables with sneeze guards, tray slides, shelves, cutting boards, and more! We offer steam table parts that will help you fix worn pieces of your equipment as well as make it more versatile. From heating elements to drain components, we have the items you need to ensure your food heats properly and water drains quickly. If you need a new cutting board, we carry replacements options, too.Also make sure to check out other great items from Eagle Group.


text:
Skip to comments.Barbra Streisand on love and politics [ ever been in love with a Republican,........]
Posted on 12/13/2012 12:53:27 PM PST by Sub-Driver
Barbra Streisand on love and politics
By CAITLIN MCDEVITT | 12/13/12 1:36 PM EST Reuters
Republicans hoping to romance Barbra Streisand are out of luck.
Asked by CNNs Piers Morgan if shes ever been in love with a Republican, the famously liberal actress replied: Never.
"Could you ever be? Morgan followed up.
"No," she replied. "Well, I mean unless there was an enormous sexual chemistry and, you know, and I had to - we never talked about politics, maybe. But I can't quite imagine it, no."
(Excerpt) Read more at politico.com ...
"Republicans need to put their differences aside and rebuild this nation"
"Gooba Gabba One of Us One of Us"
There is no tolerance for diversity.
Stalinists lie. Always.
George Orwell was aware of this and wrote several books about it. Apparently people never got the message.
You're good at that ... or used to be.
Another open minded Hollywood liberal.
Tell us what in your 6th grade education qualifies you to understand politics, Barbra ?
It's more like, 'A Republican could NEVER be in love with Barb!'
I used to LOVE it when a Freeper posted excerpts from her website; the grammar and spelling errors were HILARIOUS!
Why are they asking her this? Did James Brolin finally sober up and dump her ample libtard @ss?
That said, God did give her an amazing gift...
In my wildest hallucination or my worst drunken state, I wouldn't touch her.
If some how it would somehow happen, I would immediately take a bath, then I would repent, apologize to everyone I know and then submit to a public hanging, for atonement.
I could never be that stoned or drunk?!
What conservative or pub would even want to be in the same building with Babs!
Could you imagine waking up with this snuggled up to you?
I'd chew my damn arm off just to get out from under her and make my escape.
unless there was an enormous sexual chemistry....................................... Uuh? I'd need a jar full of Viagra just to get started with her. then again, I'd doubt if that would work.
Mrs./Ms. B.S, whatever they call it now. :p, can kiss me hairy a... erm... hairy feet.. she ranks pretty much alongside Hanoi Jane and Purple/Pink Kerry..
They can all lay on a busy highway, for all I care..
Jon Peters!
Apparently it is not essential that celebrities be able think clearly, nor that they be able to age well.
Babs, the feeling is mutual.
I am embarrassed and slightly ashamed that I was thinking the exact same thing (including exact same picture)...
It's not that people didn't get the message, it's that liberals looked at them as instruction manuals, and conservatives looked at them as the warnings they were.
Well, to be fair, every last one of us will look something like that eventually. Time is no friend to youthfulness.
Barbra: then stop being so bi-partisan in your concerts. You get us in and then you talk about how "we are all Americans" and then you dump on a large portion of the population when the tour is over.
The words "sexual chemistry" and "Barbra Streisand" in the same paragraph is pretty nauseating...
True... that includes myself, but she was always a bowser and an insufferable leftist to boot.
Good grief. She could render useless a double dose of Viagra.
Diana, I rarely disagree with you but I am now. I've never considered her talented or even a singer that I like to hear.
I suppose "amazing gift", like beauty, is in the eyes and ears of the beholder.
On the flip side, I do not find the conservative Dennis Miller the slightest bit humorous either, ditto Bob Hope.
On the other hand, when I first read my tag line, a quote from Larry Elder, I literally laughed out loud.
Reminds me of the Priest in Harold and Maude....
"Now, Harold, the Church has nothing against the union of the old and the young. Each age has its own beauty. But a marital union is concerned with the conjugal rights. And the procreation of children. I would be remiss in my duties if I did not tell you that the idea of -- (he swallows) - intercourse - the fact of your young, firm --(growing disturbed) -- body commingling with those withered flesh, sagging breasts, and flabby buttocks - makes me --(falls apart) - want to vomit."
Think Hillary Clinton 4 years from now, hideous imagery but somehow comforting though it might not should be that way, America will not elect the hag because she's.......a hag.
Fine. We can disagree. I like her older songs and her movies, 'Funny Girl' and 'Funny Lady' but the latter mostly for Omar Sharif! *SWOON*
But PLEASE tell me that you DO NOT find 'The Three Stooges' funny! OMG! I'll NEVER get them! It has to be that 'X-Y Chromosome Thing' between men and women because I'll NEVER get it. Ever. *SHRUG*
Never have, nor do I find videos funny where people get hurt, like landing on their heads or men getting "racked", people slipping on ice and falling, etc.
Thank Goodness! :)
That being?
klik Peeple!
I don't think she will ever have to worry about that. Looking at her recent photos I would have to say HAS ANYONE EVER SEEN BARBAR AND SHEMP IN THE SAME ROOM TOGETHER.
Roses are red,
Violets are blue.
You have a nose
Like a B-52.
xxx, td
Babs putting that enormous snozze into my business is snotty...
She plays that thing like a flute.. drawing rats of every kind..
The Pied Piper of Moscow.. maybe Peking..
And Piers with his gun control blather is so illogical he trips over his tongue..
They both have Logical Tourettes.. with Autistic tendencies..
Yes, we all know her husband.
I listened to Brolin's live interview on WPLR the day he said it..
He wished everyone a "Happy 9/11". He and Her are perfect for each other.
Her role as communist SHJ Katie Morosky (ironic name?) in "The Way We Were" was off-putting enough. We don't need to hear constant reprises of the role . . .
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
amol, 22 May 2012it is very very bad phone....
touch screen is very bad.
it is waste of money.hai,
i would like to buy this one, whats the problems this phone
it is very very bad phone....
touch screen is very bad.
it is waste of money.
were can i find the "document viewer" in this phone...i haven't found that yet..but its given in the phone's specification
It is waste mobile.I think not only this samsung is waste. Go for Nokia. Previously i used Nokia Xpress music compare to Nokia X music it doesn't have 50% software options. i think if hardware is getting problem i.e model(C3312) problem. But in this software is like in china mobile not only this model all samsung mobiles have this problem. I found no difference between samsung and china mobiles. Larger(size more than 1MB) images are not opened.Music library is worst,We can't set image as wallpaper if image size is 300K. Samsung providing worst software for images,audio and videos.
Anonymous, 20 May 2012I am much interested in this phone...im having one doubt,does this phone has document viewer..... morehi.samsung 3312 duos is a gd budget phone with nice social networking apps like facebook,instant messager,chat on,orkut,twitter.it will nt support pdf documents files,it cannot read&support....its best only 4 surfing net,music&videos...........
i like this phone very much but ihav 1 doubt that is does this phone has "document viewer"....can we able to read pdf and .doc files...please reply
When I receive a new Message it Shows "1 New Message"
on screen and wn I unlock the Fn Message goes to in box automatically. Please advice why it is happening why it is not showing new message with sender Name ?
I am much interested in this phone...im having one doubt,does this phone has document viewer....can we able to read pdf and .doc files...please reply asap...thanks in advance:-)
Hey users need ur help yr,
As such 3 days back i have posted a query bt no one replied yet.... i am placing the Question again for your kind reply, if possible 4 u....
"I have very little knowledge about touch screen mobile. But now i think this "Samsung C3312 Duos" can be a good one for me. But in this mobile, when try to search for a particular "contact name", what will be the search method??? means.... do I need to drag the contact list ups & down ? or there is a side bar? or there is a search option, as we can find in our general mobile set? "
please answer.....
!!Very good budget phone for this Price!!
I was looKing for a decent phone in my price range(5K).
I bought this phone for 3500 online(ebay).
Very good music and sound quality is great.
Battery bacKup is fabulous 2-3days with music.
Good display, good for watching videos.
The touch is new, i mean i cannot say it is purely resistive but has a very good touch response.
Nothing much to say about the camera but a decent one considering the price tag.( Come..on nobody would expect the cam to be a professional one....I fall in those group of people who rarely taKes pictures)
Surprisingly samsung has provided a handsfree that sounds liKe its made for music.
The only disappoint atleast for me is that the phone supports pc synch but a usb cable was not provided in the box.(A minor one, could be ignored)
My conclusion:
If u want to buy a phone with features that u will utilize 100% and u don't want to spend much on other costlier phone with many features that u wont even remember at times...(LOL) Go for this blindfolded u wont repent..
Tanq 4 readin..
vicky, 18 May 2012hai i am using samsumg champ c3312 ... in my mobile i have problem in bluetooth ..... if i tra... moreVery good phone wow...?;-)
i am the user of samsung c3312 first of all this is made by china which is not good second it has bluetooth problem third is dual sims not active together. im not satisfy this cell and im very disheart to purchase it.


text:
Fashion Pack
|"Fashion Pack (Studio 54)"
|Single by Amanda Lear
|from the album Never Trust a Pretty Face
|"Black Holes"
"Lili Marleen" (Japan, Italy)
"Forget It" (Russia)
|7", 12"
|Euro disco
|5:05 (album version)
4:23 (single edit)
|Ariola Records
|Anthony Monn, Amanda Lear
|Anthony Monn
|Amanda Lear singles chronology
"Fashion Pack" is a song by French singer Amanda Lear released in 1979 by Ariola Records.
Song information[edit]
"Fashion Pack" was released as the second single off Never Trust a Pretty Face in early 1979. "Black Holes" from the same album made the B-side on most of the single releases. The song was a collaboration between Anthony Monn, who composed the music and produced the track, and Amanda Lear, the author of the lyrics. Musically, "Fashion Pack" is a typical upbeat track of the disco era. The lyrics captured the feel of disco eminence of the late 1970s, telling about the legendary Manhattan-based Studio 54 (which was the reason for the "Studio 54" subtitle on the single cover).[1] Lear also mentioned in the song a number of celebrities who were Studio 54's regular attendees, such as Andy Warhol, Margaux Hemingway, Liza Minnelli or Bianca Jagger. Names of Vogue, Interview and Ritz magazines appear here as well as names of trendy French venues Maxim's and Chez Régine. The lyrics also refer to Yves Saint Laurent, "travolting" and haute couture. The song met with success and remains one of Amanda Lear's biggest hits to date.
Amanda Lear has re-recorded "Fashion Pack" three times in her later career. An "updated" version has made a release on her 1993 dance album Cadavrexquis. It doesn't mention most of the stars from the 1979 original and replaces their names with the likes of Madonna, Elton John and Naomi Campbell. It also drops any connotations to Studio 54.
In 1998 Lear made another remake of the song, for the Back in Your Arms album, which saw her revisiting her disco-era hits. That version retained the original lyrics.
In 2016, she recorded an new "updated" version in her album Let Me Entertain You. It doesn't mention most of the stars from the 1979 original and replaces their names with the likes of Joe Dalessandro, Kate Moss and Naomi Campbell and talk about instagram at the end.
Spanish singer Pedro Marín covered the song on his 2006 record Diamonds, a tribute album including interpretations of chosen Amanda Lear's tracks.[2]
Other versions[edit]
"Fashion Pack" was performed by Louise English and Sue Upton during the "Madame Louise Summer Collection" segment of the March 5, 1980 edition of The Benny Hill Show.[3]
Track listing[edit]
- 7" Single (1979) [4]
- A. "Fashion Pack" - 4:23
- B. "Black Holes" - 5:00
- Japanese 7" Single (1979)
- A. "Fashion Pack" - 3:37
- B. "Lili Marleen" - 4:40
- German 12" Single (1979)[5]
- A. "Fashion Pack" - 5:10
- B. "Black Holes" - 5:06
- Italian 12" Single (1979)[6]
- A. "Fashion Pack" - 5:09
- B. "Lili Marleen" - 4:45
- Russian 7" Single (1981)
- A. "Реклама вокруг нас" ("Fashion Pack")
- B. "Забудь это" ("Forget It")
Chart performance[edit]
- ^ "Studio 54 @ Disco-Disco.com". www.disco-disco.com. Retrieved 2011-05-20.
- ^ "Pedro Marin Diamonds (cd) Disco Compact Discs & MP3s". www.discomusic.com. Retrieved 2011-04-29.
- ^ "The Music of The Benny Hill Show - Page 4 (1978-1981)". www.runstop.de. Retrieved 2013-04-07.
- ^ "Amanda Lear - Fashion Pack / Black Holes (Vinyl) at Discogs". www.discogs.com. Retrieved 2011-05-19.
- ^ "Amanda Lear - Fashion Pack (Vinyl) at Discogs". www.discogs.com. Retrieved 2011-05-19.
- ^ "Amanda Lear - Fashion Pack / Lili Marleen (Vinyl) at Discogs". www.discogs.com. Retrieved 2011-05-19.
- ^ "Chartverfolgung / LEAR,AMANDA / Single". Musicline.de (in German). Retrieved 2009-10-08.
- ^ "Discografie Amanda Lear". dutchcharts.nl (in Dutch). Retrieved 2009-09-23.
- ^ "Indice per Interprete: L". www.hitparadeitalia.it (in Italian). Retrieved 2009-10-08.
- ^ "Discography Amanda Lear". swedishcharts.com. Retrieved 2009-10-08.
External links[edit]


text:
Evidence for widespread changes in promoter methylation profile in human placenta in response to increasing gestational age and environmental/stochastic factors
The human placenta facilitates the exchange of nutrients, gas and waste between the fetal and maternal circulations. It also protects the fetus from the maternal immune response. Due to its role at the feto-maternal interface, the placenta is subject to many environmental exposures that can potentially alter its epigenetic profile. Previous studies have reported gene expression differences in placenta over gestation, as well as inter-individual variation in expression of some genes. However, the factors contributing to this variation in gene expression remain poorly understood.
In this study, we performed a genome-wide DNA methylation analysis of gene promoters in placenta tissue from three pregnancy trimesters. We identified large-scale differences in DNA methylation levels between first, second and third trimesters, with an overall progressive increase in average methylation from first to third trimester. The most differentially methylated genes included many immune regulators, reflecting the change in placental immuno-modulation as pregnancy progresses. We also detected increased inter-individual variation in the third trimester relative to first and second, supporting an accumulation of environmentally induced (or stochastic) changes in DNA methylation pattern. These highly variable genes were enriched for those involved in amino acid and other metabolic pathways, potentially reflecting the adaptation of the human placenta to different environments.
The identification of cellular pathways subject to drift in response to environmental influences provide a basis for future studies examining the role of specific environmental factors on DNA methylation pattern and placenta-associated adverse pregnancy outcomes.
The human placenta is a temporary organ that facilitates the exchange of nutrients, gas and waste between maternal and fetal circulations. In order to carry out these functions, it is comprised of heterogeneous cell types including several trophoblast cell populations (cytotrophoblasts, extra-villous trophoblasts and syncytiotrophoblast), fibroblasts, mesenchymal cells, as well as fetal and maternal vascular tissue and blood cells. The extra-villous trophoblast cells must first invade the maternal decidua and remodel maternal arteries, to allow direct contact between maternal blood and the placental syncytiotrophoblast cell layer
The placenta also undergoes several physiological changes throughout gestation, with one of the most significant being the flooding of placenta villi by maternal blood at the end of the first trimester (~12 weeks gestation), resulting in a rise in oxygen concentration as well as a decrease in trophoblast invasion. It is believed that the inability of the placenta to respond to this change in oxygen concentration can lead to placental disease, such as preeclampsia
The molecular mechanisms behind these morphological and functional changes are now beginning to be understood at both the gene-specific and genome-wide level. Wide-ranging genome-wide gene expression differences between placentas at different gestational ages were reported in two recent studies
The importance of epigenetic factors in placental development and function has long been known through the study of imprinted genes
Due to its role as the interface between the mother and fetus, the placenta is exposed to a myriad of environmental factors, some of which have been shown to alter placental gene expression, as well as epigenetic marks
Several genome-scale DNA methylation studies have focused on finding tissue-specific differentially methylated regions (tDMRs) between placenta and maternal blood, as a means of detecting placental pathologies and fetal chromosomal trisomies using non-invasive methods (reviewed in
The aim of the current study was to build on recent knowledge obtained through genome-scale gene expression
Genome-scale DNA methylation analysis of first, second, and third trimester placenta
Genome-scale DNA methylation analysis of 18 first trimester (8-12 weeks), 10 second trimester (17-24 weeks) and 14 third trimester placenta (34-41 weeks) samples was performed using the Illumina Infinium HumanMethylation27 BeadChip (see Additional file
Additional file 1
Summary of placental samples analysed in the study.
Click here for file
Additional file 2
Correlations between average methylation in first, second and third trimesters. Correlations between average methylation in (A) first and second trimesters, (B) second and third trimesters, and (C) first and third trimesters. This analysis revealed that first and third trimester methylation was most discordant, as expected, while second trimester placenta is more similar to third trimester placenta in terms of overall promoter DNA methylation. Furthermore, this figure visually shows the increase in methylation in third trimester compared to first (C).
Click here for file
Figure 1
Cluster dendrogram based on all autosomal Infinium probes distinguishes placentas of different gestational age
Cluster dendrogram based on all autosomal Infinium probes distinguishes placentas of different gestational age. Dendrogram showing the relationship between placental samples from three gestational ages based on DNA methylation levels (β-values) of all analysable Infinium probes. All samples clustered within their gestational age group, with no overlap between gestations, suggesting there are consistent genome-scale DNA methylation patterns associated with each gestational age. First trimester samples clustered away from second and third trimester samples, indicating that overall Infinium methylation patterns are more similar in second and third trimester compared to first trimester.
Additional file 3
Correlation between Infinium and Sequenom methylation levels. Correlation between Infinium HumanMethylation27 BeadChip and Sequenom EpiTYPER locus-specific methylation analysis. Methylation levels in 12 genes were measured using Sequenom MassARRAY Epityping targeting the same CpG sites interrogated on the Infinium BeadChip Arrays. Correlation between platforms was 0.76, supporting the use of the Infinium HumanMethylation27 BeadChip for profiling DNA methylation in this study. Genes interrogated are listed in Additional file
Click here for file
Additional file 4
Sequenom EpiTYPER primer sequences.
Click here for file
Transition to normoxia is not associated with major changes in placental DNA methylation profile
Despite the overall interspersed pattern of clustering of first trimester samples of various gestations (8 - 12 weeks) (Figure
Additional file 5
Unsupervised clustering of first trimester placenta based on differentially methylated probes between 8 and 12 weeks gestation. HeatMap showing unsupervised clustering of 8 and 12 week placenta samples based on 12 probes that showed a Δβ > 0.2 between 8 week and 12 week placenta. The 12 probes were associated with 11 genes, with two probes associated with the
Click here for file
Gestational age is associated with promoter methylation levels
In order to gauge the effects of gestational age on the overall methylation level at gene promoter regions enriched on the Infinium HumanMethylation27 BeadChip, the mean methylation across all probes (Methylation Index - MI) was calculated for each sample (Figure
Figure 2
Average methylation of all samples for first, second and third trimester
Average methylation of all samples for first, second and third trimester. Methylation Index (MI) was calculated for each sample by calculating the mean of all analysable Infinium β-values (26, 162 probes) for that sample. The MIs were then grouped by gestation and shown as box and whisker plots. First and second trimester placentas show a similar overall level of methylation (p = 0.46) with median MIs of 0.238 and 0.241, respectively. Third trimester samples show significantly elevated average MI values (median = 0.256) relative to both first and second trimester, indicating that there is a significant increase in methylation level from second to third trimester.
Additional file 6
Proportion of probes within a particular methylation level. Pie Charts showing the proportion of probes within a particular methylation level for first, second and third trimester. The percentage of probes with a 'β < 0.02' is the same in all three gestational ages (63%), suggesting that probes with low methylation in first trimester remain low over placental development. Furthemore our data suggests that probes with an intermediate methylation in first trimester are the ones that increase over gestation, with a lower proportion of probes in the 'β = 0.2 - 0.6 group' in second (22%) and third (20%) compared to 1st trimester (24%); and a higher proportion of probes in the β > 0.6 group in 2nd (15%) and term (17%) placenta compared to 1st trimester (13%).
Click here for file
Absolute differences in mean methylation between first and second, first and third, and second and third trimesters were generally small, with only 149, 954 and 157 probes respectively, showing Δβ > 0.2 (Table
Table 1
Number of probes showing differential methylation between first, second and third trimester placental tissue.
Differentially methylated probes
(adj. p < 0.05)
Difference β ≥ 0.1 and adj. p < 0.05
Difference β ≥ 0.2 and adj. p < 0.05
First v Second
8, 240
411 ↓
1, 077 ↑
12 ↓
137 ↑
First v Third
8, 298
755 ↓
2, 581 ↑
71 ↓
883 ↑
Second v Third
7, 669
288 ↓
1, 515 ↑
6 ↓
151 ↑
↑: Increase in methylation; ↓: Decrease in methylation
Additional file 7
Average methylation level of probes that increase in methylation over gestation. Box plot showing average methylation of (A) all probes (n = 26, 162) in first, second and third trimester placenta, and (B) probes that shown an increase in methylation from first to third trimester of β > 0.2 (n = 883). This analysis shows that probes with intermediate levels of methylation in first trimester are the ones that increase over time. On the other hand, probes with low methylation (β < 0.2) in the first trimester do not appear to increase in methylation in the third trimester placenta.
Click here for file
Figure 3
Unsupervised clustering based on probes with Δβ > 0.2 between First and Third trimester
Unsupervised clustering based on probes with Δβ > 0.2 between First and Third trimester. HeatMap showing unsupervised clustering of all placenta samples (x-axis) based on 954 probes with a Δβ > 0.2 between First and Third trimester (y-axis). The majority of differentially methylated probes show higher methylation in third trimester (883 probes) compared to only 71 probes with lower methylation in third trimester. Second trimester placentas cluster as a separate group, and show a methylation profile that is an intermediate of first and third trimesters. Green corresponds to low methylation and Red to high methylation.
Changes in cell composition, primarily a decrease in cytotrophoblasts, from first to third trimester could be one the major factors contributing to methylation differences between the two gestational time points. In order to test this, methylation levels between purified first trimester cytotrophoblasts and first and third trimester placenta, were examined using both Infinium and Sequenom EpiTYPER analyses (22 data points) (Additional file
Additional file 8
Correlation between methylation levels in purified first trimester cytotrophoblasts and first and third trimester placenta. Scatter plot showing correlation (r2) between first and third trimester placenta and purified first trimester cytotrophoblasts methylation, based on Infinium HumanMethylation27 BeadChip and Sequenom EpiTYPER analysis. The correlations between first trimester placenta and cytotrophoblasts were (A) 0.96 and (C) 0.93, and between third trimester placenta and cytotrophoblasts were (B) 0.88 and (D) 0.88, using Infinium and Sequenom platforms, respectively. This finding suggests that both first and third trimester placenta methylation levels are indicative of cytotrophoblast levels. The lower correlation in third trimester is likely due to both lower numbers of villous cytotrophoblasts and their differentiation into the syncytiotrophoblast layer.
Click here for file
Differentially methylated genes between first trimester, second trimester and term
Probes that showed differences of Δβ > 0.2 (approximating 20% differential methylation) between each of the three gestational ages, (Table
Table 2
Top Canonical Pathways from IPA for probes showing differential methylation across gestation
First v Second trimester
# differentially methylated genes/# genes in the pathway
Communication between Innate and Adaptive Immune Cells
Role of Cytokines in Mediating Communication between Immune Cells
Altered T Cell and B Cell Signaling in Rheumatoid Arthritis
Calcium Signaling
Crosstalk between Dendritic Cells and Natural Killer Cells
Second v Third trimester
# differentially methylated genes/# genes in the pathway
Systemic Lupus Erythematosus Signaling
Crosstalk between Dendritic Cells and Natural Killer Cells
Role of NFAT in Regulation of the Immune Response
Agrin Interactions at Neuromuscular Junction
Wnt/β-catenin Signaling
First v Third trimester
# differentially methylated genes/# genes in the pathway
Communication between Innate and Adaptive Immune Cells
Systemic Lupus Erythematosus Signaling
Role of Cytokines in Mediating Communication between Immune Cells
Altered T Cell and B Cell Signaling in Rheumatoid Arthritis
Crosstalk between Dendritic Cells and Natural Killer Cells
Inter-placental methylation variation increases with gestational age
DNA methylation may be modulated in part by environmental influences and may serve as a mediator between the environment and genome function (reviewed in
Figure 4
Number of variable probes increases with gestation
Number of variable probes increases with gestation. The number of probes with high inter-individual variation increases over gestation. Variance (
Additional file 9
Number of probes showing variation at each gestational age.
Click here for file
Further analysis revealed that most of the gestational age associated variation was found in probes with intermediate methylation (0.2 < β < 0.6) rather than low (β < 0.2) or high (β > 0.6) methylation (Additional file
Additional file 10
Relationship between probe methylation level and variation. Relationship between methylation level and inter-individual variation at each gestational age (
Click here for file
While inter-individual variation of DNA methylation may in part reflect genetic polymorphisms
Figure 5
Variance levels of probes in the third compared to the first trimester
Variance levels of probes in the third compared to the first trimester. Scatter plot of probe variance (
Additional file 11
Number of probes showing variation at each gestational age. Venn diagram of variable probes (
Click here for file
The top pathways for genes that show variable methylation at each gestastional age are listed in Table
Table 3
Top Canonical Pathways for gene-associated probes that show variable methylation within each gestational age
Variable in First trimester
Arachidonic Acid Metabolism
Hepatic Fibrosis/Hepatic Stellate Cell Activation
Circadian Rhythm Signaling
Calcium Signaling
Nitrogen Metabolism
Variable in Second trimester
Circadian Rhythm Signaling
Glutathione Metabolism
Arachidonic Acid Metabolism
Sonic Hedgehog Signaling
Metabolism of Xenobiotics by Cytochrome P450
Variable in Third trimester
Glutamate Receptor Signaling
Valine, Leucine and Isoleucine Degradation
β-alanine Metabolism
Butanoate Metabolism
Tyrosine Metabolism
CpG density and genomic context influence variability in placental methylation
In order to examine the relationship between CpG density and methylation status, probe locations were assigned to either CpG Island (CGI) or non-CpG Island (non-CGI) genomic regions, as annotated by Illumina. Using a chi square test, we found that probes showing increased methylation across gestation were enriched for non-CGI regions (χ2 = 480.2, p < 0.001), while probes showing lower methylation in third compared to first trimester were not context dependent (χ2 = 2.68, p = 0.14 (Additional file
Additional file 12
Relationship between DNA methylation and genomic context. Probes were separated into two groups based on their genomic location - CpG Island (CGI) or non-CpG Island (non-CGI). The expected frequency was based on the proportion of all analysable Infinium probes (A) within a CGI or outside a CGI (0.76 and 0.24, respectively). Probes that increased in methylation over gestation were predominantly in non-CGI regions (B), while probes that decreased in methylation over gestation showed the expected proportions. Furthermore, probes that showed inter-individual variation in (D) both first and third trimesters, (E) first trimester only, and (F) third trimester only, were predominantly associated with CGIs. This finding further suggests that probes that change over gestation are not the same as those that show inter-individual variation within each gestation.
Click here for file
DNA methylation profile influences global gene expression in the placenta
In order to assess the overall effect of DNA methylation on gene expression profile, methylation β-values levels for first, second and third trimester placental tissue were correlated with publicly available expression data for matched gestational age placenta
Additional file 13
Relationship between DNA methylation and gene expression levels. Relationship between DNA methylation and gene expression levels in first, second and third trimester placenta. Infinium HumanMethylation BeadChip probes were quartiled into 4 groups (0-25%, 25-50%, 50-75%, 75-100%) based on methylation level, with the same number of probes in each quartile. The quartiles for each gestational age were plotted on the x-axis with the corresponding gene expression values obtained from publically available first, second and third trimester data (y-axis). This analysis shows a decreasing median gene expression level with increasing DNA methylation, highlighting the functional relevance of DNA methylation in placenta at all three gestational ages.
Click here for file
Figure 6
Correlation between methylation and gene expression change between first and third trimester
Correlation between methylation and gene expression change between first and third trimester. Methylation difference (Δβ) between first and third trimester (x-axis) was plotted against gene expression log fold change (y-axis) between first and third trimester. A positive change in log fold expression indicates higher expression in first trimester, while a positive change in methylation indicates higher methylation in the third trimester. Therefore, the top left panel includes genes which showed lower methylation and higher expression in first compared to third trimester. The three highlighted genes (CCR7, GNLY and CCL21) ranked highly in IPA analysis. Grey dots represent Infinium probes. Black dots represent specific genes of interest.
Additional file 14
Correlation between methylation and expression change between first and third trimester with more genes highlighted. Methylation difference (Δβ) between first and third trimester (x-axis) was plotted against gene expression log fold change (y-axis) between first and third trimester. A positive change in log fold expression indicates higher expression in first trimester, while a positive change in methylation indicates higher expression in third trimester. Highlighted genes are those that show a correlation between methylation and expression level. Grey dots represent Infinium probes, black dots represent most differentially methylated and expressed genes.
Click here for file
In this study we performed genome-wide DNA methylation analysis of gene promoter regions, using the Illumina Infinium HumanMethylation27 BeadChip, in placentas of different gestational ages (18 first trimester (8-12 weeks), 10 second trimester (17-23 weeks), and 14 third trimester samples (34-41 weeks)). We further validated the array data by targeting 12 CpG sites (covering 12 gene promoters) using the Sequenom EpiTYPER platform. The correlation of r2 = 0.76 between the two platforms is comparable to that published for similar comparisons
Unsupervised clustering based on all Infinium probes (excluding those on the sex chromosomes) clearly separated all samples by gestational age, with first trimester samples clustering away from second and third trimester samples (Figure
Despite the interspersed clustering of first trimester samples based on the overall Infinium methylation patterns, we were interested in assessing DNA methylation changes occurring at the transition from the first to the second trimester. This period of placental development is characterised by the loosening of trophoblast plugs and the associated rise in oxygen concentration from 2-3%, to 7-8%
The separation of the trophectoderm from the inner cell mass at the blastocyst stage occurs at a time when genome-wide DNA methylation levels are at their lowest
IPA analysis of genes showing differential methylation between first and third trimesters indicated that the most affected pathways were 'communication between innate and adaptive immune cells' and other '
In addition to identifying CpG sites that consistently change over gestation, we were also interested in CpG sites that show inter-individual variability within each gestational age. Genes that show inter-individual differences in expression in placenta have previously been described by Sood et al.
Finally, contrary to CpG sites that increase in methylation across gestation, variable CpG sites are more likely to be located within CpG islands (Additional file
The direct role of DNA methylation in controlling placental global gene expression levels was examined by comparing our DNA methylation data with published gene expression data for placenta tissue of the same gestational age (Figure
In the present study, we performed genome-scale DNA methylation analysis of human placenta at three distinct gestational ages, using the Infinium HumanMethylation27 array. This platform targets over 14, 000 genes, however, is limited to the 5' promoter region. Our findings support the hypothesis that DNA methylation levels in the human placenta are dynamic and change over gestation, possibly in response to changing cellular composition and/or cumulative environmental influences. The identification of pathways that are likely to be affected by the latter (i.e. those that show higher variation at full term) will provide valuable candidates for testing in studies examining placenta-associated adverse pregnancy outcomes.
Sample collection
Use of cells and DNA isolated from 8- and 12-week gestational tissue of normal pregnancies was approved by the Cambridgeshire Research Ethics committee (CREC 04/Q0108/23). Additional 1st trimester (8-12 week gestation) and 2nd trimester (17-24 week gestation) placental tissue was collected from elective abortions with the approval from the ethics committees of the University of British Columbia and the Children's & Women's Health Centre of British Columbia. Term placental tissue was collected as previously described
DNA extraction
Tissue samples were incubated at 50°C overnight with shaking in DNA extraction buffer (100 mM NaCl, 10 mM Tris.HCl pH8, 25 mM EDTA, 0.5% (w/v) SDS), containing 200 μg/ml proteinase K. DNA was isolated by two rounds of phenol:chloroform extraction, followed by RNAse A treatment, precipitation in absolute ethanol containing 10% (v/v) sodium acetate (3 M, pH 5.2), and resuspended in 100 μl nuclease-free water (Ambion, Austin, TX, USA) or using salting out method followed by purification with Qiagen blood and tissue kit (Qiagen, Mississauga, ON, USA). DNA was stored at -20°C.
Infinium DNA methylation analysis
Infinium arrays were hybridized and scanned as per manufacturer's instructions (Illumina, San Diego, USA). Individual probe β-values (range 0-1) were are approximate representations of the absolute methylation percentage of specific CpG sites within the sample population. The values were derived by comparing the ratio of intensities between the methylated and unmethylated alleles using the following formula:
Where Signal B is the array intensity value for the methylated allele and Signal A is the non-methylated allele. Samples were processed using the Bioconductor package
Gene Ontology and Pathway analysis
Data sets were interrogated using the Ingenuity Pathways Analysis (IPA) application (Ingenuity® Systems, Redwood City, CA;
Locus-specific methylation analysis
Sequenom MassARRAY EpiTYPING was performed to validate Infinium methylation, as previously described
Raw data obtained from MassArray EpiTYPING was cleaned systematically using an R-script with the following three criteria (in order): (1) removal of samples that failed across 100% of CpG sites, (2) removal of CpG sites that failed (for example due to low or high mass, silent peaks, or low intensity readings) across more than 40% of samples, and (3) removal of samples that failed to generate data for more than 70% of CpG sites tested.
Publicly available gene expression microarray data analysis
Gene expression data was downloaded from Gene Expression Omnibus (Barrett and Edgar, 2006, Sayers et al., 2009), (Database issue D885-D890). CEL files were downloaded from series GSE9984
Authors' contributions
BN analysed the data, participated in critical discussion, and wrote the draft manuscript. LG processed the array data and performed data analysis. RKCY, JMC, RS and WR designed the study, participated in critical discussion and wrote the manuscript. MSP set-up experiments and processed array data. AS and AM provided first trimester villi samples. All authors approved of the final manuscript.
We would like to thank Dr. Dan Diego-Alvarez and Ruby Jiang for their technical assistance and Kristal Louie for placenta donor recruitment in Vancouver.
BN is supported by an NHMRC (Australia) Dora Lush Biomedial Postgraduate Scholarship. RKCY is supported by a graduate student scholarship from the Child & Family Research Institute. The Vancouver data was funded by a Canadian Institutes for Health Research Grant (to WPR). The Murdoch Childrens Research Institute is supported by the Victorian Government's Operational Infrastructure Support Program.
The anatomy of the normal placenta
Fetal tolerance in human pregnancy--a crucial balance between acceptance and limitation of trophoblast invasion
Placental origins of preeclampsia: challenging the current hypothesis
Placental-related diseases of pregnancy: Involvement of oxidative stress and implications in human evolution
Gene expression profiling of the human maternal-fetal interface reveals dramatic changes between midgestation and term
Profiling gene expression in human placentae of different gestational ages: an OPRU Network and UW SCOR Study
The importance of imprinting in the human placenta
Imprinted genes in the placenta--a review
Epigenetics in the placenta
Review: Complicated interactions between genes and the environment in placentation, pregnancy outcome and long term health
Transposable elements: targets for early nutritional effects on epigenetic gene regulation
Maternal epigenetics and methyl supplements affect agouti gene expression in Avy/a mice
Effect of maternal tobacco smoke exposure on the placental transcriptome
Quantitative methylation analysis of developmentally important genes in human pregnancy losses after ART and spontaneous conception
Altered global gene expressions of human placentae subjected to assisted reproductive technology treatments
Epigenetic approaches for the detection of fetal DNA in maternal plasma
Systematic search for placental DNA-methylation markers on chromosome 21: toward a maternal plasma-based epigenetic test for fetal trisomy 21
A microarray-based approach for the identification of epigenetic biomarkers for the noninvasive diagnosis of fetal disease
Sites of differential DNA methylation between placenta and peripheral blood: molecular markers for noninvasive prenatal diagnosis of aneuploidies
Fetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21
Human placental-specific epipolymorphism and its association with adverse pregnancy outcomes
Inter- and Intra-Individual Variation in Allele-Specific DNA Methylation and Gene Expression in Children Conceived using Assisted Reproductive Technology
DNA methylation profiling of human placentas reveals promoter hypomethylation of multiple genes in early-onset preeclampsia
Gestation-dependent changes in human placental global DNA methylation levels
Gene expression patterns in human placenta
DNA methylation profiling of human placentas reveals promoter hypomethylation of multiple genes in early-onset preeclampsia
Isolation of human extravillous trophoblast cells by attachment to laminin-coated magnetic beads
Plasma 25-hydroxyvitamin D levels in early-onset severe preeclampsia
Genomic surveys by methylation-sensitive SNP analysis identify sequence-dependent allele-specific DNA methylation
Genome-wide DNA methylation profiling using Infinium assay
Cell specific patterns of methylation in the human placenta
Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system
Temporal and regional changes in DNA methylation in the embryonic, extraembryonic and germ cell lineages during mouse embryo development
Tissue-specific differences in DNA methylation in various mammals
The 5-methylcytosine content of highly repeated sequences in human DNA
Granulysin produced by uterine natural killer cells induces apoptosis of extravillous trophoblasts in spontaneous abortion
Nutritional programming of the metabolic syndrome
Development of sucrase activity: effect of maternal hormonal status and fetal programming of jejuno-ileal differences
The relation of small head circumference and thinness at birth to death from cardiovascular disease in adult life
The fetal and infant origins of adult disease
Ischaemic heart disease in England and Wales around the year 2000
Placenta-specific methylation of the vitamin D 24-hydroxylase gene: implications for feedback autoregulation of active vitamin D levels at the fetomaternal interface
lumi: a pipeline for processing Illumina microarray
Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis
Methylation of the adenomatous polyposis coli (APC) gene in human placenta and hypermethylation in choriocarcinoma cells
The isolation and characterization of a population of extravillous trophoblast progenitors from first trimester human placenta
Wide ranging DNA methylation differences of primary trophoblast cell populations and derived-cell lines: implications and opportunities for understanding trophoblast function


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I'm building this site for a client that basically wants their own version of this: http://graphicnovel-hybrid4.peugeot.com/start.html
Ideally, I would like to use superscrollorama (http://johnpolacek.github.io/superscrollorama/) but I can't figure out how to switch to horizontal scrolling in the middle of the page. I tried using waypoints (http://imakewebthings.com/jquery-waypoints/#about) to change the direction, which worked but the animations stopped working in the horizontal section.
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Any help is appreciated! Thanks in advance!


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The competition has been a real laugh for UConn women's basketball over the last two seasons.
You can rest easy, Beer Mat Flipping Guy and Backspinning Dude, the UConn women have given up their seats at the table of record-breaking 71s and popped a squat next to MJ and his 72-game winning Bulls. The Huskies beat the West Virginia Mountaineers tonight to take home their 16th Big East title and extend their consecutive wins streak to 72 games.
UConn's incredible run started on November 16th, 2008 and has continued on for 477 days. On top of winning all 72 games by double-digits, the Huskies trailed in the second half for a total of only 3:37 during their streak. With UConn's last loss coming 100 weeks ago to Stanford, Huskies coach Geno Auriemma has now reached Ron Popeil status: "Set it, and forget it!" Auriemma and Co. are just 16 wins shy of tying UCLA's 88-game win streak set back in 1974. And while that wouldn't take place until next season, it makes us wonder, what would it take to stop these women?
ESPN's Digger Phelps knows a thing or two about playing spoiler; maybe he could step in as a guest coach for UConn's opposition? While coaching Notre Dame, Phelps' Irish beat seven No. 1 ranked teams including one historic juggernaut. On January 19th, 1974, Phelps coached the Fighting Irish to a 71-70 win over John Wooden's Bruins, ending college basketball's longest winning streak of all-time. Besides being an X's and O's wizard, Phelps can create quite the distraction with his pregame dance ritual and that might be exactly what's needed to throw UConn superstar Maya Moore off her game.
UConn's opponent could also try casting a bad luck spell on the Huskies. How about the Curse of Destruction? The curse is said to deliver unbearable pain, mental and physical on whomever it's cast. However, the spell only works if you're polite to Mr. Moon. As spellsofmagic.com explains, "RESPECT THE MOON OR ELSE THIS CURSE WILL NOT WORK! TREAT THE MOON THE WAY YOU WANT TO BE TREATED!"
If neither option works, the powers that be should think about promoting the Huskies to a tougher league. If UConn women win the 2010 NCAA Tournament, the WNBA should adopt them as a franchise. Huskies center Tina Charles and guard Kalana Greene are likely first-round picks in this year's WNBA Draft while junior forward Maya Moore is certainly on pace to be the No. 1 overall selection in 2011.
Ultimately, it's going to take a monster effort to stop these women. If the streak remains intact at the end of this season, we have one request of the NCAA: Schedule the record-breaking game versus the Bruins next year. Having 99-year-old Wizard of Westwood, John Wooden, in attendance for the possible 89th win would be a magical moment in college hoops for all.


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Handmade Craft Ideas from 2010: A Year of Free Crafts
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How to Make a Unique Place Setting Utensil Holder
By: Linda Peterson
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Are you tired of boring old table linens? Celebrate spring and summer by learning how to make a unique place setting utensil holder and transform your table from Linda Peterson! This is a cheap and easy project that anyone can try.
Start with an old, clearn garden glove. Instead of throwing it away or stuffing it in a drawer, use fabric paint and other embellishments to give it a second life! Make a set of these from a variety of old gloves - it's a great way to create a unique twist on traditional table crafts. Use the glove as a napkin or let it hold utensils - it's that versatile!
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Welcome, Chicago Skeptics!
Skeptical Salon: The Signal & the Noise: Why So Many Predictions Fail
We're back on for the Skeptical Salon; please note the new location! This month, we'll be reading The Signal and the Noise: Why So Many Predictions Fail--but Some Don't... Learn more
- Jennifer and Jeff's
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Believing in Things We Cannot See
Is belief without sight rational? God may be beyond human comprehension, but then again, so is most science - at least to us mere mortals (with Liberal Arts degrees).... Learn more
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According to Meetup, this group was started in September 2006. That means it's one of those Big Deal Anniversary things, right? So let's have a party! Same time, same... Learn more
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Oakleigh House Nursery
Now taking registrations for 2016-2017 and beyond.
Click here to see our new Nursery film
Clubs and Activities
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Best for your child...best from your child
A Co-educational Independent Primary School in Swansea
Oakleigh House School is a co-educational independent primary school taking pupils from 2 years 6 months to 11 years of age. It is situated in the Uplands area of Swansea adjoining the beautiful Cwmdonkin Park. It houses a separate nursery department, a multi-purpose hall, modern dining facilities and a range of specialist classrooms. Throughout school the children develop their ICT skills by using iPads and laptops. There is plenty of outdoor space for the children to play and a nature area for them to explore.
Oakleigh House School benefits from a distinctive family atmosphere with a caring ethos and a positive attitude to learning. Expectations are high, with pupils of all abilities challenged to achieve their full potential in a secure, supportive environment.
There is an excellent pupil teacher ratio and a dedicated team of staff are well qualified to deal with children across the age range. Teachers plan a broad, modern curriculum, set clear goals and attach great importance to good manners and the development of self-discipline.
Latest News
Paralympic Rio 2016 Torch Tour29 June 2016
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Summer Fair at Oakleigh House24 June 2016
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Jordi Amat donates shirt to school16 May 2016
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Presented in part at the Veterinary Orthopedic Society meeting, February 20–27, 2010, Breckinridge, CO.
Original Article - Clinical
Evaluation of Topical Epidural Analgesia Delivered in Gelfoam for Postoperative Hemilaminectomy Pain Control
Version of Record online: 5 DEC 2012
DOI: 10.1111/j.1532-950X.2012.01082.x
© Copyright 2012 by The American College of Veterinary Surgeons
Veterinary Surgery
Volume 42, Issue 1, pages 79–84, January 2013
Additional Information
How to Cite
Barker, J. R., Clark-Price, S. C. and Gordon-Evans, W. J. (2013), Evaluation of Topical Epidural Analgesia Delivered in Gelfoam for Postoperative Hemilaminectomy Pain Control. Veterinary Surgery, 42: 79–84. doi: 10.1111/j.1532-950X.2012.01082.x
Publication History
- Issue online: 3 JAN 2013
- Version of Record online: 5 DEC 2012
- Manuscript Accepted: SEP 2011
- Manuscript Received: JUL 2010
- Abstract
- Article
- References
- Cited By
To evaluate efficacy of a topical epidural analgesia used alone, or in combination with hydromorphone, against a standard pain protocol for the 48 hours immediately after hemilaminectomy.
Study Design
Randomized, blinded, controlled, clinical trial.
Dogs (n = 30) with thoracolumbar intervertebral disc disease treated with hemilaminectomy.
Dogs were randomly divided into 3 groups: group 1 received intermittent hydromorphone postoperatively, group 2 received a topical epidural of preservative-free morphine and dexmedetomidine administered via gel foam, group 3 received both forms of analgesia. All dogs were monitored and assessed for pain for 48 hours immediately postoperatively. Data were analyzed using MANOVA and Wilcoxon Rank Sum Tests.
There was a significant temporal difference in treatment groups 1 and 3 when using a 0–10 pain scale (MANOVA, P = .02). There was also a significant difference at the 48th hour postoperatively between groups 1 and 3 (Wilcoxon Rank Sum Test).
Topical epidural of preservative-free morphine and dexmedetomidine administered via gelfoam is not sufficient analgesia alone post hemilaminectomy but in conjunction with other opioid administration may lead to superior pain relief.


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Tracy MorganWed, June 5, 2013 - 8:00 PMFletcher Hall at The Carolina Theatre Durham in Durham, NC
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A meal made of meats or spreads, generally placed between two slices of leaven grain product. (exception sometimes one slice of leaven grain product is used resulting in a open faced sammich.
Wow! I'm hungry I'm gonna grab a SAMMICH!
Beküldő: Hoth20 2004. június 7.
A sandwich made with pure love, typically by a Sgt that cares.
the Sgt made you a sammich now eat it! that's an order!
Beküldő: icebluecan 2012. június 28.
Hillbilly slang for those who cannot speak correctly.
Here is you beer and sammich.... now make sure ya'll brushes that toothe before ya hit that thar sack
Beküldő: Irishninja 01 2011. március 30.
A sammich is the coolest possible way to spell "Sandwich", in doing so you also have to pronounce as so:
I ate a sammich, it tasted good epic win on my part.
Beküldő: sparta kicking person 2011. május 25.
1. Any form of the common sandwich that is made by someone else, thus elevating it to new amazing levels.
Hey floor, make me a sammich!!!
Beküldő: Fisch 2006. december 12.
The best sandwich in the world, which has been prepared by a woman. Known to cause mouth orgasms and non-stop NOM-ing.
Woman: Here's your sandwich babe.
Man: Mmmmmm SAMMICH!!! OM NOM NOM NOM!!!
Man: Mmmmmm SAMMICH!!! OM NOM NOM NOM!!!
Beküldő: ummmmmmmmmmmmmmm...... 2011. április 19.
An extremely delicious sandwich prepared for a man by his woman, either waiting for him on the table when he gets home from work or delivered to him while he is relaxing on the couch.
I'ma have to choke a bitch if my sammich ain't ready when I get home.
Hey, why don't you take a break from blowing me for a minute and go make me a sammich, babe.
Hey, why don't you take a break from blowing me for a minute and go make me a sammich, babe.
Beküldő: AbomB320 2010. augusztus 1.