text:
|Publication number
|Publication type
|Application number
|Publication date
|Jul 14, 1998
|Filing date
|Oct 4, 1996
|Priority date
|Oct 4, 1996
|Fee status
|Publication number
|Raimo Harju, Mikko Vaisala
|Original Assignee
|Wallac Oy
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (1), Referenced by (99), Classifications (10), Legal Events (4)
|External Links: USPTO, USPTO Assignment, Espacenet
1. Field of the Invention
The present invention relates generally to optical scanners and more particularly to a scanner type apparatus for imaging different biochemical samples distributed on flat supports i.e. chromatographic and electrophoretic patterns for visualization and computer analysis.
2. Description of Prior Art
In many biochemical methods of separation and analysis the distribution and quantity of various proteins or other molecules on a plane type base will be measured. Typical examples of such methods are gel electrophoresis and planar chromatography. More recent methods are, for example, hybridization's and various kinds of cell separation methods carried out on filter materials.
Detecting for few molecules as such on a given surface is extremely difficult. In biochemistry the substances examined must be labelled with a label or a tracer to facilitate identification or detection.
Until the past decade, various radioactive labels have been practically the only type of the labels used, and still today a large number of routine research is carried out with isotopic labels. Only in recent years have various non-isotopic labels, like chemiluminescent and fluorescent labels been adopted more widely for use.
The detection of two-dimensional distribution of radioactive labels is most often performed by contact imaging with photographic film. This method is called autoradiography. During the last five years the film has been partly substituted by a special ceramic-coated plate called photostimulable phosphor image plate. These plates have been exposed like a film but image of the stored radiation have been read out with a special scanner. Some of these instruments are disclosed for example in the following U.S. Pat. Nos. 3,859,527; 4,258,264; 4,302,671 and 4,346,295. Typically the most modern commercial phosphor image plate scanners are using red laser beam, which is scanned with an oscillating mirror and it is focused into a flat surface with a special scan lens also called theta-lens. The consequent near-ultra-violet light emission will be collected and conducted to the detector with a bundle of fibers or with a special plastic light guide.
There is also available a different phosphor image plate readout device which uses scanning optical probe technology. In this instrument both the stimulating light and the emission light will be conducted via optical fiber and the excitation is focused onto the sample surface using so called GRIN-lens. This instrument is disclosed in U.S. Pat. No. 5,266,803.
The fluorescence and the fluorescent labels have been used quite many years in planar chromatography and these samples have been read with different type of scanning fluorescent densitometers, one example is disclosed in U.S. Pat. No. 4,117,338.
However, the main interest here is the gel electrophoresis with fluorescent labels. Scanning fluorescent densitometers are also capable to read fluorescent labelled gels, but they have not been used much there probably due to their low sensitivity, low resolution and slow scanning speed. Most of the fluorescent work with gels have been utilized ethidium bromide labelling and visual inspection or Polaroid film documentation on top of UV-light box. Polaroid film cameras have been replaced now by low noise solid state video cameras and video printers.
If the question is of the quantitative determination of protein with fluorescent labels the above described methods are not sufficient. For quantitative purposes the sophisticated high resolution fluorescent scanner has been commercialized by Molecular Dynamics and disclosed in U.S. Pat. No. 5,424,841. Another same type instrument is commercialized by Hitachi Software Engineering Co. and its features are disclosed in U.S. Pat. No. 5,190,632.
Although these instruments have been basically constructed by the same way as the phosphor image plate scanners, utilizing laser beam scanning, these instruments were not easily adopted for phosphor image plate reading. The excitation wavelength of fluorescence scanners differ so much from the stimulation wavelength of phosphor image plate scanners that the laser itself and also many other optical components had to be changed. It means that to build a combine instrument utilizing laser beam scanning would be expensive and impractical.
In U.S. Pat. No. 5,091,652 (Mathies et al) there is disclosed a laser excited confocal fluorescent scanner and a method. The confocal principle was adopted from microscopes to the gel scanning. They did achieve good resolution and high sensitivity but its realization was mechanically very demanding in large sample sizes like 200 mm * 250 mm due to the small working distance, the small image depth. Also the practical gels are often only few millimeters thick and their surface is uneven.
In a slightly different field of automated DNA-sequencers there have been utilized also various gel scanners. There the gels will be read during the separation process itself, thus the measuring probe needs to move only in one direction. The excitation light is often supplied in to the moving probe using mirrors moving in collimated laser beam. Two of these instruments have been disclosed in U.S. Pat. Nos. 4,811,218 (Hunkapiller et al.) and 5,100,529 (Fujii).
The same principle is applied in the high speed fluorescence scanner disclosed in U.S. Pat. No. 5,459,325 (Hueton et al.). However, this principle performs well only with collimated laser beam and in relative short distances between the excitation source and scan head.
The reading of molecular fluorescence and the reading of stimulated phosphorescence are the same type of measurement methods and they have been used for the same analytical purposes in the same laboratories. Therefore it would be an advantage if these measurements could be performed with the same instrument, the output image files would be of the same type and different methods could be easily compared.
It is therefore an object of the present invention to provide a compact multi-purpose apparatus for quantitative reading of different types of flat samples in the field of biochemistry. These type of samples include for example:
phosphor image plates capable of storing radiation image information from isotope labelled gels, blots, TLC-plates etc.,
fluorescent labelled gels, filters etc., regardless of the type of the labels whether they are based on the classical molecular fluorescence or the time-resolved luminescence,
microtitration plates with fluorescent samples,
colored planar samples.
These objects can be accomplished by providing an apparatus for reading optical emission or reflection intensities from stimulated phosphor image plates, excited fluorescently labelled samples or colometricly labelled samples. The apparatus is comprising:
a measurement unit,
a sample support plate,
means for performing relative movement between the measuring head and the sample on the sample support.
The measurement unit is comprising:
a fiber coupled light source allowing excitation of broad selection of fluorescent labels. Preferable it is a xenon arc point source, equipped with a chopper for millisecond region time-resolved applications or a pulsed xenon source for more demanding time-resolved applications,
and a measurement head.
The measurement head is comprising:
a fixed light source preferably a semiconductor laser for stimulation of phosphor image plates or fluorescence excitation of suitable red or near-infrared fluorochromes,
another optical input located on the opposite side of the fixed light source,
an objective lens for focusing incident light and for collecting emitted or reflected light from the sample,
a holder for interchangeable filter cubes and a set of different filter cubes,
a sensitive detector preferably a head-on photomultiplier,
and detection electronics including linear amplifier, analogue-to-digital converter and the computer interface.
FIG. 1 shows a preferred embodiment of the multi-purpose optical scanner.
FIG. 2 shows a configuration of the filter cube used in phosphor image plate reading mode.
FIG. 3 shows a configuration of the filter cube for fluorescence reading mode.
FIG. 4 shows a configuration of the filter cube for reading mode of colored plate samples.
FIG. 5 shows a configuration of the filter cube for infrared fluorescence reading mode.
FIG. 1 shows a preferred embodiment of the multi-purpose optical scanner. The preferred embodiment of the present invention in FIG. 1 comprises following units: a measurement head comprising a fixed light source unit 10, fiber input unit 30 and the emission unit 40, the fiber-coupled light source 20 and a sample compartment 50 including the scanning mechanism.
The fixed light source unit 10 has a small collimated semiconductor laser 11 as a light source with wavelength about 630-670 nm. A lens 12 focuses the laser beam in to a pinhole 13. An achromatic lens 14 collimates the light from the pinhole 13 through a beamsplitter 15 in to an emission unit 40. The beamsplitter 15 reflects a small portion of the light into a reference photodiode 16. The output of the reference photodiode 16 is used to stabilize the optical power of the laser 11. The electronics of the system is not shown.
The fiber coupled light source 20 has a broad band discharge light source 21, preferably DC xenon short arc lamp, a spherical back reflector 22 to gain more effective light collection, a hot mirror 23 for reflecting infrared energy out of the beam, achromatic lenses 24 and 25 to collect the light from lamp and to focus the light into an optical fiber 28. In the vicinity of the input aperture of the fiber there is located a chopper plate 26 rotated by a motor 17. The optical fiber 28 isolates mechanically and thermally the bulky high power discharge light source from the measurement head. A fiber input unit 30 comprises a lens 31 that collimates the light from fiber 28 through a beamsplitter 32 into an emission unit 40. The beamsplitter 32 reflects a small portion of the light through a lens 33 which focuses the light into a reference photodiode 34. The output of the reference photodiode 34 is used to stabilize the optical power of the arc source 21. The electronics is not shown.
The emission unit 40 comprises a large numerical aperture objective lens 41 which in this embodiment is simple glass aspheric lens, a filter cube holder 42, a detector 43, which could be of a small modern metal-can type photomultiplier, a linear amplifier 44, an analogue-to-digital converter 45 and a computer interface card 46. The high voltage for the photomultiplier is supplied by a power supply 47.
The sample compartment 50 includes a sample support plate 51 and a sample 52. Below the sample support plate 51 there is a scanning mechanism 53, not shown in FIG. The double arrows 54 show the orthogonal scanning directions.
The filter cube used inside the filter holder 42 are application dependent and basic types of different filter cubes are shown in FIGS. 2-5.
FIG. 2 shows a filter-cube 60 which is used when measuring phosphor image plates. This filter cube comprises dichroic mirror 61 and emission filter 62. The dichroic mirror 62 is tilted 45 degrees towards the beam coming from the fixed light source and it reflects the light downward into the input aperture of the objective lens.
FIG. 3 shows a filter-cube 70 which is used in most typical fluorescence application. This filter cube comprises dichroic mirror 71 and emission filter 72 and excitation filter 73. The dichroic mirror 71 is not tilted 45 degrees towards the beam coming from the filter input unit 30 it reflects the light downward into the input aperture of the objective lens 41. There is needed a different filter-mirror combination for different fluorescent labels with different spectral characteristics.
FIG. 4 shows a filter-cube 80 which is used when reading colored plates common in thin-layer chromatography. This filter cube comprises non-dichroic beamsplitter mirror 81 and bandpass filter 83 to choose desired absorptive wavelength to the reflection measurement. Mirror 81 is tilted 45 degrees towards the beam coming from the fiber input and it reflects a portion of the light downward into the input aperture of the objective lens. The objective focuses the light on to the sample and it is partly absorbed and partly reflected depending on the concentration of stain on TLC-plate.
FIG. 5 shows a filter-cube 90 which is used at certain infrared fluorescence application. This filter cube comprises dichroic mirror 91 and emission filter 92. The dichroic mirror 91 is not tilted 45 degrees towards the beam coming from the semiconductor laser and it reflect the light downward into the input aperture of the objective lens 41. No excitation filter is needed as the semiconductor laser light is enough monochromatic. There is needed a different filter-mirror combination for other fluorescent labels with different spectral characteristics.
Description will now be made of a process in which the multi-purpose scanner, as herein above described, is used to form image of different types of samples.
Phosphor image plate reading
Phosphor image plate reading is performed using the fixed light source unit 10 and related filter cube 60. Most common used phosphor image plates are stimulated by red light and consequent luminescence is at the blue and near ultraviolet (360-460 nm) region of the spectrum. The collimated beam of 1.5 mW laser diode 11 of wavelength 670 nm is focuses with plano-convex glass lens 12 in to the pinhole 13 of the diameter of 100 micrometers. The laser beam out of pinhole 13 is collimated with anti-reflection coated achromatic lens 14 (f=25 mm) and this collimated beam is reflected downwards with the mirror 61 located in the filtercube 60. Mirror 61 is a dichroic mirror, where red light is reflected and blue light transmitted.
The objective lens 41, in this embodiment a standard anti-reflection coated aspheric lens of focal length 18 mm, is focusing the beam on to the surface of the sample 52. The spot size on the phosphor image plate is about the size of the pinhole. The amplified output from reference photo diode 16 is fed to the power supply of the laser diode for stabilization purposes. The stabilization of the laser when reading phosphor image plates is extremely important because the fluctuations of the laser power are difficult to compensate in reading results due the unlinear relation between emission and stimulation.
The whole phosphor image plate is scanned with this spot line by line. Typical phosphor image plate size is 250�200 mm and if the scanning resolution is five lines/mm, totally 1000 lines have to be scanned to form a whole image. Highest reasonable scanning speeds with ball-screws are about 0.5 m/s and with stops and turns and accelerations outside the sample area the reading of one line takes about one second.
The blue emission light from stimulated phosphorescence crystals of the phosphor image plate is collected with high numerical aperture aspheric objective lens 41. This lens has multi-layer antireflection coating which is optimized at the region of the laser wavelength. In phosphor image plate scanner most of stimulation light is scattered out of the phosphor image plate surface and this scattered light have to be absorbed somewhere, so that it cannot get back again on to the surface of phosphor image plate. The scattered stimulation light would erase the phosphor image plate and information of the part of the plate would be lost.
The objective lens 41 collimates the emission light through the emission filter 61 in the filter cube 60 in to the detector 43. Because the amount of scattered stimulation light is much bigger than the real signal the emission filter 61 in the filter cube 60, has critical demands. It should attenuate the read stimulation light about two magnitudes but should have a good transmission for blue emission light. The glued stack of color filter glasses have a good blocking characteristics for stimulation light but its transmission is only about 30% for emission light. The specially designed thin film short pass filter combined with absorptive glass, is far more effective.
Because the emission light levels from stimulated phosphor image plates are low the photomultipliers are generally used as detectors 43 in phosphor image plate scanners. The simulation light of wavelength of 670 nm has extra advantage with certain photomultipliers that the photo-electric converter layer of photomultiplier tube, photocathode, has best sensitivity on the blue region of the optical spectrum and very poor sensitivity at the wavelength of 670 nm thus helping the filter design.
If the pixel size is square there is 1250 readings or pixels in one line, which make A/D-conversion frequency of 2500 Hz. Due the large inherent dynamic range of phosphor image plate there is needed a 16-bit A/D-conversion. Thus the image file size is 2.5 Mbytes. Images are analyses in PC with a separate program.
Fluorescence reading
As a simple molecular process the fluorescence emission is stimulated or excited at lower wavelength light than subsequent emission. Also there is available a huge number of fluorescent labels with different emission and excitation wavelengths. One group of labels which use is increasing, are the so called infra-red (IR) labels. These labels are excited at the red end of the visible spectrum and their emission wavelengths are at the near-infrared or region electromagnetic spectrum. Very often read laser diodes are capable to excite IR-labels. For this reason the described embodiment (FIG. 1) of the invention is capable to image certain IR-fluorescence label using filter cube 90. In this also the detector 43 must be near-infrared sensitive which has some disadvantages in phosphor image plate reading.
However most fluorescent labels in molecular biology are excited in the ultra-violet, blue or green region of the optical spectrum. Most flexible and effective excitation sources for these labels are different type of short arc discharge lamp, both flash type or continuous wave type. But because these lamps are often bulky and generate extra heat they are most suitable for fiber optic coupling. This kind of embodiment is shown as a part in FIG. 1, but now must use filter cube 70.
One common fluorescent label is fluorescein and its derivatives, which excitation and emission wavelength are 490 and 515 nm, respectively. The excitation light from arc lamp 21 is collected and collimated with mirror 22 and a lens 24 then light is focused with a lens 25 into the optical fiber 28. The fiber output is collimated into the dichroic mirror 71 in the filter cube 70. The mirror 71 directs the excitation light downwards in to the objective 41. The reference beamsplitter 32, a thin uncoated glass plate reflects about 8% of the beam into the lens 33 which focuses the reference beam on to the surface of the photo-diode 34. With arc sources, due small arc wandering, it is important that the whole beam is probed for reference. The output of amplified reference signal is used to stabilize the arc source output. If the used excitation source is difficult to stabilize the reference output can also be used for correction of the fluorescence signal, if fluorescence is measured in the linear region of the label. The dichroic mirror 71 must be specially designed for each label, so that it reflects excitation wavelength but transmits emission wavelengths. Also the emission filter 73 must be specially designed band-pass filter for the label to achieve good enough signal-to-noise ratio of the measurements.
If the label is excited at ultra-violet region of optical spectrum transmission properties of the whole optical excitation path must be carefully considered. Often the optical component must be high-purity synthetic fused silica to avoid background fluorescence from component itself and to allow good transmission properties.
If the label used is so called time-resolved, for example europium-chelate, its unique properties could be utilized using optical chopper plate 26 with continuous lamp or a flash lamp (not shown). The decay times of lanthanide chelates are typically from 100 μs to few milliseconds. When decay times are at that region the time-resolved measurements are easily accomplished by chopping excitation light mechanically and delaying reading after excitation pulse for a certain time. Above mentioned scanning speeds would allow only one excitation pulse per pixel and chopping frequency of 2.5 kHz. In this case the decay time of the label would affect resolution in direction of scanning line.
In some application the whole fiber coupled light source could be change fiber coupled laser, if the excitation band matches the laser line. The laser have advantage of getting higher excitation powers into smaller fiber. Smaller fibers means overall better possible resolution and high power allows higher scanning speeds.
However, what is not shown here, it is also common practice, for example in flow cytometers, to use two more detectors with application specific dichroic mirrors allowing the detection of two or more fluorescent labels every with characteristic spectral properties.
Colored plate samples
Reflection mode allows the multi-purpose scanner to analyze also stained thin-layer chromatography plates and for instance silver-stained electrophoretic gels. The fiber coupled light source 21 can be used for reflective measurement with a filter cube 80. The broad band light from source 21 is collected with a lens 24 and focused with lens 25 into the optical fiber. The from other end of the fiber light is collimated with the lens 31 into the mirror 81 in the filter cube 80 which is now located in the filter cube holder 42 of the emission unit 40. The mirror 81 directs the light downward and the lens 41 focuses it on to the sample surface 52. Depending on the surface absorption properties variable amounts of light is reflected out of surface, from which a certain share is collected by the lens. There is no need for any emission filter because the is no background light to filter presuming the light shield box is tight. In any case the design opto-mechanics components and optical coating have to do carefully to avoid any internal reflection inside the instrument itself.
The whole plate is scanned with light spot and amounts reflected light is recorded pixel by pixel. These values can be converted using known equation for absorption values which are linearly related to the concentration of the stain in the sample.
There is another embodiment according to this invention where the sample is moving only one direction performing only one-dimensional scan and the measuring head itself is performing the scan which is orthogonal against the first one. Relative small measurement head could be easily moved back and forth with frequency of 1 or 2 Hz. In this embodiment the programmable automatic changer of filter cubes could be easily arranged. The cube not in use at the moment could be located in a rateable carousel, from where a new filter cube could be picked when needed. The cube can also be a part of a slide unit.
Although the invention has been described with reference to the phosphor image plate or electrophoretic gels, it is equally applicable to any form of sample matrix like multi-well plates etc.
The invention has been described with reference to a preferred embodiments. It is to be understood that variations or modifications may be easily made by anybody of ordinary skill in the art without departing from the scope of this invention which is defined by the appended claims.
|Cited Patent
|Filing date
|Publication date
|Jul 24, 1995
|Jun 18, 1996
|Molecular Dynamics, Inc.
|Compact scan head with multiple scanning modalities
|Citing Patent
|Filing date
|Publication date
|Jul 28, 1997
|Mar 9, 1999
|Applied Imaging, Inc.
|Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration
|Jul 16, 1998
|Feb 15, 2000
|Ljl Biosystems, Inc.
|Moveable control unit for high-throughput analyzer
|Jul 16, 1998
|Mar 7, 2000
|Ljl Biosystems, Inc.
|Floating head assembly
|Dec 30, 1997
|Mar 28, 2000
|Bio-Rad Laboratories, Inc.
|Multi parameter scanner
|Apr 17, 1998
|Jun 6, 2000
|Ljl Biosystems, Inc.
|Light detection device
|Aug 31, 1998
|Dec 12, 2000
|Ljl Biosystems, Inc.
|Sample transporter
|Sep 2, 1998
|Feb 13, 2001
|Ljl Biosystems, Inc.
|Chemiluminescence detection device
|Feb 13, 1998
|Feb 20, 2001
|Hatachi, Ltd.
|Multicolor fluorescence detection type electrophoretic analyzer
|Dec 29, 1998
|May 1, 2001
|Applied Imaging, Inc.
|Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration
|Oct 16, 1998
|Jun 5, 2001
|U.S. Philips Corporation
|Optical system with anti-reflection coating
|Jan 28, 2000
|Oct 2, 2001
|Ljl Biosystems, Inc.
|Methods and apparatus for detecting nucleic acid polymorphisms
|Jul 16, 1998
|Nov 6, 2001
|Ljl Biosystems, Inc.
|Optical filter holder assembly
|Jan 22, 2001
|Nov 13, 2001
|Ljl Biosystems, Inc.
|Frequency-domain light detection device
|Aug 18, 2000
|Dec 4, 2001
|Ljl Biosystems, Inc.
|Broad range light detection system
|Apr 25, 2001
|Apr 9, 2002
|Micron Technology, Inc.
|Method and apparatus for automated, in situ material detection using filtered fluoresced, reflected, or absorbed light
|Mar 19, 1999
|Apr 9, 2002
|Board Of Regents, The University Of Texas System
|Fiber-optic confocal imaging apparatus and methods of use
|Mar 14, 2000
|Aug 20, 2002
|Olympus Optical Co., Ltd.
|Laser microscope
|Jan 19, 2001
|Oct 15, 2002
|Ljl Biosystems, Inc.
|Apparatus and methods for spectroscopic measurements
|Jul 31, 2000
|Oct 22, 2002
|Molecular Devices Corporation
|Detection device for light transmitted from a sensed volume
|Jan 19, 2001
|Nov 19, 2002
|Ljl Biosystems, Inc.
|Apparatus and methods for time-resolved spectroscopic measurements
|Jan 5, 2000
|Dec 3, 2002
|Ljl Biosystems, Inc.
|Sample-holding devices and systems
|Dec 3, 2001
|Dec 24, 2002
|Ljl Biosystems, Inc.
|Broad range light detection system
|Aug 31, 1998
|Dec 31, 2002
|Ljl Biosystems, Inc.
|Sample feeder
|Oct 5, 2000
|Apr 8, 2003
|Perry Sandstrom
|Microarray detector and synthesizer
|Jun 23, 1999
|Apr 15, 2003
|Regents Of The University Of Minnesota
|Confocal macroscope
|Jan 5, 2001
|Apr 29, 2003
|Axon Instruments, Inc.
|Scanning microscope
|Aug 17, 2000
|May 20, 2003
|Able Signal Company Llc
|Microarray detector and synthesizer
|Apr 29, 1999
|Jun 10, 2003
|Ljl Biosystems, Inc.
|Chemiluminescence detection method and device
|Nov 4, 1997
|Mar 9, 2004
|Micron Technology, Inc.
|Method and apparatus for automated, in situ material detection using filtered fluoresced, reflected, or absorbed light
|Apr 5, 2002
|Aug 24, 2004
|Fuji Photo Film Co., Ltd.
|Biochemical analysis data producing method, biochemical analysis data producing apparatus and stimulable phosphor sheet used therefor
|Apr 8, 2003
|Oct 19, 2004
|Able Signal Company, Llc
|Microarray detector and synthesizer
|Nov 19, 2001
|Nov 23, 2004
|Thermogenic Imaging, Inc.
|Apparatus and methods for infrared calorimetric measurements
|Sep 7, 2001
|Nov 23, 2004
|Wallac Oy
|Optical instrument and process for measurement of samples
|Nov 10, 2000
|Nov 30, 2004
|Molecular Devices Corporation
|Multi-mode light detection system
|Mar 7, 2002
|Dec 14, 2004
|Micron Technology, Inc.
|Method and apparatus for automated, in situ material detection using filtered fluoresced, reflected, or absorbed light
|Feb 5, 2001
|Dec 28, 2004
|Flir Systems Boston, Inc.
|Apparatus and methods for infrared calorimetric measurements
|Sep 7, 2001
|May 10, 2005
|Wallac Oy
|Optical instrument and process for measurement of samples
|Dec 30, 2002
|Aug 2, 2005
|Olympus Optical Co., Ltd.
|Microscope system
|May 16, 2002
|Jan 3, 2006
|Applera Corporation
|Lens assembly for biological testing
|Sep 7, 2001
|Apr 4, 2006
|Wallac Oy
|Intelligent instrumentation with changeable optical components
|Oct 19, 2004
|Jul 25, 2006
|Able Signal Company, Llc
|Microarray detector and synthesizer
|Jun 4, 2004
|Sep 5, 2006
|Micron Technology, Inc.
|Method and apparatus for automated, in situ material detection using filtered fluoresced, reflected, or absorbed light
|May 9, 2002
|Jan 30, 2007
|Fuji Photo Film Co., Ltd.
|Biochemical analysis data producing method and scanner used therefor
|Aug 26, 2004
|May 1, 2007
|Fujifilm Corporation
|Semiconductor laser device and radiation image read-out method and apparatus
|Aug 28, 2003
|Jul 31, 2007
|Waters Investments Limited
|Fluorescence detector geometry
|Jun 3, 2004
|Oct 23, 2007
|Oc Oerlikon Balzers Ag
|Optical device for surface-generated fluorescence
|Jan 14, 2003
|Jun 3, 2008
|Piper James R
|Method and/or system for analyzing biological samples using a computer system
|Aug 24, 2005
|Jul 8, 2008
|Dr. Chip Biotechnology Incorporation
|Optical detection apparatus and method thereof
|Mar 31, 2005
|Aug 5, 2008
|Applera Corporation
|Lens assembly for biological testing
|Jul 25, 2006
|Apr 21, 2009
|Able Signal Company, Llc
|Microarray detector and synthesizer
|Sep 15, 2005
|Mar 23, 2010
|Fujifilm Corporation
|Radiation image read-out apparatus
|Sep 13, 2006
|Apr 27, 2010
|National University Corporation Hamamatsu University School Of Medicine
|Microscopic cell observation and inspection system using a plurality of observation methods
|Jan 25, 2010
|Nov 30, 2010
|Wallac Oy
|Enhanced instrumentation and method for optical measurement of samples
|Feb 6, 2008
|Nov 26, 2013
|Vysis, Inc.
|Method and/or system for analyzing biological samples using a computer system
|Jun 17, 2010
|Dec 16, 2014
|W.O.M. World Of Medicine Ag
|Device and method for multi-photon fluorescence microscopy for obtaining information from biological tissue
|Sep 13, 2005
|Oct 13, 2015
|Applied Biosystems, Llc
|Achromatic lens array
|May 10, 2012
|Apr 12, 2016
|Applied Biosystems, Llc
|Lens assembly for biological testing
|Mar 15, 2013
|Jun 21, 2016
|The Boeing Company
|Method and system for detecting exposure of composites to high-temperature
|Apr 5, 2002
|Nov 7, 2002
|Fuji Photo Film Co., Ltd.
|Biochemical analysis data producing method, biochemical analysis data producing apparatus and stimulable phosphor sheet used therefor
|Sep 7, 2001
|Mar 13, 2003
|Petri Aronkyto
|Advanced instrumentation for optical measurement samples
|Sep 7, 2001
|Mar 13, 2003
|Raimo Harju
|Accurate instrumetation for optical measurement of samples
|Sep 7, 2001
|Jun 26, 2003
|Raimo Harju
|Intelligent instrumentation for optical measurement of samples
|Feb 19, 2003
|Aug 14, 2003
|Regents Of The University Of Minnesota
|Confocal macroscope
|Dec 30, 2002
|Aug 21, 2003
|Olympus Optical Co., Ltd.
|Microscope system
|Jan 14, 2003
|Sep 11, 2003
|Vysis, Inc., A Corporation Of The State Of Delaware
|Method and/or system for analyzing biological samples using a computer system
|Apr 8, 2003
|Sep 18, 2003
|Perry Sandstrom
|Microarray detector and synthesizer
|May 16, 2002
|Nov 20, 2003
|Sandell Donald R.
|Lens assembly for biological testing
|Jun 4, 2004
|Nov 11, 2004
|Mark Eyolfson
|Method and apparatus for automated, in situ material detection using filtered fluoresced, reflected, or absorbed light
|Aug 28, 2003
|Mar 3, 2005
|Gilby Anthony C.
|Fluorescence detector geometry
|Aug 26, 2004
|Mar 3, 2005
|Fuji Photo Film Co., Ltd.
|Semiconductor laser device and radiation image read-out method and apparatus
|Jun 3, 2004
|Mar 10, 2005
|Unaxis Balzers Ltd.
|Optical device for surface-generated fluorescence
|Aug 25, 2004
|Apr 14, 2005
|Flexgen Technologies B.V.
|Apparatus for treatment of light-sensitive biopolymers
|Oct 19, 2004
|Apr 14, 2005
|Perry Sandstrom
|Microarray detector and synthesizer
|Mar 31, 2005
|Aug 4, 2005
|Applera Corporation
|Lens assembly for biological testing
|Sep 13, 2005
|Jan 12, 2006
|Applera Corporation
|Achromatic lens array
|Sep 13, 2005
|Jan 12, 2006
|Applera Corporation
|Lens assembly for biological testing
|Sep 15, 2005
|Mar 16, 2006
|Fuji Photo Film Co., Ltd.
|Radiation image read-out apparatus
|Jul 25, 2006
|Nov 16, 2006
|Perry Sandstrom
|Microarray detector and synthesizer
|May 9, 2002
|Nov 30, 2006
|Fuji Photo Film Co., Ltd.
|Biochemical analysis data producing method and scanner used therefor
|Aug 24, 2005
|Mar 8, 2007
|Dr. Chip Biotechnology Incorporation
|Optical detection apparatus and method thereof
|Sep 10, 2004
|Oct 25, 2007
|Wallac Oy
|Instrumentation and Method Adapted For Optical Measurement of an Amplified Luminescent Proximity Homogeneous Assay
|May 17, 2007
|Nov 22, 2007
|Luminex Corporation
|Chip-Based Flow Cytometer Type Systems for Analyzing Fluorescently Tagged Particles
|Sep 13, 2006
|Feb 26, 2009
|Hideo Mogami
|Microscopic Cell Observation and Inspection System Using a Plurality of Observation Methods
|Oct 27, 2008
|Apr 30, 2009
|Sandstrom Perry L
|Microarray detector and synthesizer
|Jan 25, 2010
|May 20, 2010
|Wallac Oy
|Enhanced instrumentation and method for optical measurement of samples
|Jun 17, 2010
|Apr 5, 2012
|W.O.M. World Of Medicine Ag
|Device and method for multi-photon fluorescence microscopy for obtaining information from biological tissue
|Apr 2, 2012
|Oct 11, 2012
|Duerr Dental Ag
|Device for Reading Storage Films
|Mar 15, 2013
|Sep 18, 2014
|The Boeing Company
|Method and system for detecting exposure of composites to high-temperature
|Nov 19, 2014
|Mar 12, 2015
|W.O.M. World Of Medicine Ag
|Device and method for multi-photon fluorescence microscopy for obtaining information from biological tissue
|Jun 11, 2013
|Jun 4, 2015
|Sharp Kabushiki Kaisha
|Fluorescence detection device
|May 18, 2001
|Dec 19, 2001
|Oki Electric Industry Co., Ltd.
|Optical device for use in detecting the wavelength and intensity of light
|May 18, 2001
|Jul 14, 2004
|Oki Electric Industry Co., Ltd.
|Optical device for use in detecting the wavelength and intensity of light
|May 31, 2006
|Feb 28, 2007
|DR. Chip Biotechnology Incorporation
|Optical detection apparatus and method thereof
|Aug 11, 1998
|Feb 25, 1999
|Bio-Rad Laboratories
|Multi parameter scanner
|Sep 24, 1999
|Jun 2, 2000
|Xerion Pharmaceuticals Gmbh
|Method and device for automated chromophore-assisted laser/light inactivation (cali)
|Dec 2, 2000
|Jun 7, 2001
|Evotec Oai Ag
|High rate screening method and device for optically detecting samples
|Dec 2, 2000
|Dec 13, 2001
|Evotec Biosystems Ag
|High rate screening method and device for optically detecting samples
|Feb 25, 2003
|Sep 25, 2003
|Carl Zeiss Jena Gmbh
|Assembly and method for an imaging spectrometer
|May 20, 2005
|Dec 1, 2005
|J & M Analytische Mess- Und Regeltechnik Gmbh
|Device for carrying out thin-film chromatography
|U.S. Classification
|250/458.1, 250/583
|International Classification
|Cooperative Classification
|European Classification
|May 4, 1998
Owner name: WALLAC OY, FINLAND
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:HARJU, RAIMO;VAISALA, MIKKO;REEL/FRAME:009171/0454
Effective date: 19980417
|Dec 20, 2001
|Fee payment
Year of fee payment: 4
|Dec 27, 2005
|Fee payment
Year of fee payment: 8
|Jan 14, 2010
|Fee payment
Year of fee payment: 12


text:
According to Ned Davis Research, $100 invested in all dividend payers of the S&P 500 index in 1972, would have grown to $2,266 by the end of 2009. The same $100 invested in non-dividend paying stocks in the S&P 500 returned a negative 39% over the same period. The performance of dividend payers and initiators was even better, returning $2,945 on the initial investment in 1972. Dividend investors should utilize every edge they could find in order to deliver above average total returns. As a result, the findings of the Ned Davis study should not be ignored. The reason why dividend growers outperform is that they represent an elite group of companies which grow earnings, reinvest some of it in the business, and distribute the rest to stockholders. Rising profits equal rising stock prices over the long run, and rising dividends as well.
Some of the companies which raised distributions over the past week include:
The Clorox Company (CLX) engages in the production, marketing, and sales of consumer products in the United States and internationally. The company raised dividends by 10% to 55 cents/share. This was the thirty-third consecutive year of dividend increases for this dividend aristocrat. The stock yields 3.40%. (analysis)
First Financial Corporation,(THFF) through its subsidiaries, provides various financial services in Indiana and Illinois. The company raised its semi-annual dividend by 2.20% to 46 cents/share. This dividend achiever has managed to increase dividends to shareholders for 22 consecutive years. The stock yields 3.20%.
Bunge Limited (BG) engages in the agriculture and food businesses worldwide. The company approved a 9.5% increase in the company's regular quarterly cash dividend, from $0.21 to $0.23 per share. The company, which is a member of the international dividend achievers, has consistently raised dividends since 2003. The stock yields 1.90%.
Transatlantic Holdings, Inc. (TRH), through its subsidiaries, offers reinsurance capacity for a range of property and casualty products, directly and through brokers, to insurance and reinsurance companies, in domestic and international markets. The dividend was raised by 5% to 21 cents/share. The Board of Directors has raised the quarterly distributions of this dividend achiever every year since TRH became a public company in 1990. The stock yields 1.80%.
Canadian Pacific Railway Limited (CP), through its subsidiaries, provides rail and intermodal freight transportation services. The company increased its next quarterly dividend to 27 Cents/share from 24.75 cents per share. This international dividend achiever has consistently raised distributions since 2004. The stock yields 1.90%.
Republic Bancorp, Inc. (RBCAA) operates as the holding company for Republic Bank & Trust Company and Republic Bank, which provides banking, tax refund solutions, and mortgage banking services to individuals and businesses in the United States. The company announced an 8% increase in the Company's second quarter cash dividends to $0.143 per share. This dividend achiever has consistently raised dividends since 1999. The stock yields 2.30%
Dr Pepper Snapple Group, Inc. (DPS) operates as a brand owner, manufacturer, and distributor of non-alcoholic beverages in the United States, Canada, Mexico, and the Caribbean. The company raised its quarterly dividend by 67% to 25 cents/share. This was the first dividend increase since the company initiated a dividend payment policy in 2009. The stock yields 2.60%.
Nordstrom, Inc., (JWN) is a fashion specialty retailer, which offers apparel, shoes, cosmetics, and accessories for women, men, and children in the United States. The company announced that its board of directors declared a quarterly dividend of $0.20 per share, an increase of 4 cents or 25% over the previous quarter's dividend. This was the first dividend increase since 2008.The stock yields 2.10%.
W.R. Berkley Corporation (WRB), operates in the property casualty insurance business in the United States and internationally. The company increased its cash dividend to an annual rate of 28 cents per share, representing a 17% increase from the present rate. The company's annual dividends have increased for 5 years in a row. The stock yields 4.20%.
Knight Transportation, Inc. (KNX), together with its subsidiaries, operates as a short to medium-haul truckload carrier of general commodities in the United States. The company raised distributions by 20% to 6 cents/share. The company has consistently raised dividends since 2004. The stock yields 1.20%.
Analog Devices, Inc. engages in the design, manufacture, and marketing of analog, mixed-signal, and digital signal processing integrated circuits used in industrial, communication, computer, and consumer applications. The company's Board of Directors increased quarterly dividend by 10% to $0.22 per share. The company's annual dividends have increased every year since 2004. The stock yields 3.10%.
Tiffany & Co.(TIF), through its subsidiaries, engages in the design, manufacture, and retail of fine jewelry. Its jewelry products include gemstone jewelry, gemstone band rings, diamond rings, wedding bands for brides and grooms, non-gemstone, gold or platinum jewelry, and sterling silver jewelry. The company announced a 25% increase in its quarterly dividend from $0.20 to $0.25/share. This is the second announced increase in the quarterly dividend payment policy since the start of the calendar year. The company's annual dividend has increased every year since 2003. The stock yields 2.30%.
Ship Finance International Limited (SFL) , through its subsidiaries, owns and operates vessels and offshore related assets in Bermuda, Cyprus, Malta, Liberia, Norway, the United States, Singapore, the United Kingdom, and the Marshall Islands. The company raised distributions by 10% to 33 cents/share. Before you get too excited about the current yield of 7.30%, please bear in mind that the current dividend is half what it were in Q3 2008.
Unum Group (UNM), together with its subsidiaries, provides group and individual disability insurance products primarily in the United States and the United Kingdom. The company announced that its Board of Directors authorized an increase of 12.1% in the quarterly dividend to 9.25 cents/share. The stock yields 1.60%.
Ashland Inc. (ASH)operates as a specialty chemicals company internationally. The company raised annual distribution by 100% to 60 cents/share. The stock yields 1.10%.
Safeway Inc.(SWY), together with its subsidiaries, operates as a food and drug retailer in North America. The company approved a 20% increase in its quarterly dividend from $0.10 per share to $0.12 per share. This is the sixth consecutive annual dividend increase for the company. The stock yields 2%.
Xcel Energy Inc.,(XEL) through its subsidiaries, engages in the generation, purchase, transmission, distribution, and sale of electricity to residential, commercial, industrial, and public authorities in the United States. The board of directors raised the quarterly dividend on the company's common stock from $0.245 per share to $0.2525 per share. The company has raised distributions for 7 consecutive years. The stock yields 4.80%.
Northrop Grumman Corporation (NOC) provides products, services, and integrated solutions in the aerospace, electronics, information and services, and shipbuilding sectors. The company raised distributions by 9.30% to 47 cents/share for a seventh consecutive year. The stock yields 3%.
Full Disclosure: Long CLX
This article was featured on Carnival of Personal Finance #259: Memorial Day Edition
Relevant Articles:
- What Dividend Growth Investing is all about?
- Clorox (CLX) Dividend Stock Analysis
- How to Uncover Hidden Dividend Gems
- Are Dividend Investors Stock Pickers?
Monday, May 24, 2010
Why Dividend Growth Stocks Rock?
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subset: commoncrawl


text:
Fire and Ice is a 1983 American animated adventure-fantasy film directed by Ralph Bakshi. The film, a collaboration between Bakshi and Frank Frazetta, was distributed by 20th Century Fox, which also distributed Bakshi's 1977 release, Wizards. The animated feature, based on characters Bakshi and Frazetta co-created, was made using the process of rotoscoping, in which scenes were shot in live action and then traced onto animation cels. Wikipedia
Nekron: Get that garbage out of here.
|1 point


text:
Reviewed By Robert C (Oakhurst, CA) Reviewed for a 2004 GMC Yukon XL —2/10/2010 12:54 PM
Very good unit for the money
Reviewed By McDaniel W (Pearl River, LA) Reviewed for a 2004 Honda Element —7/12/2016 6:35 AM
I got this compressor a couple of weeks ago and have used it 2 or 3 times. I'm impressed with its ability to air up low tires and I like the automatic shut off feature when you switch it to that feature. All in all I like it and will carry it with me in my truck for emergencies. Great little compressor!!


text:
Soccer 02 May
Leicester City fans go absolutely crazy after incredible Premier League victory
They have done it.
They have done it.
Arsenal beat unlikely Premier League-leaders Leicester 2-1 thanks to a last-second goal by striker Danny Welbeck on Sunday. It was a (…)
Premier League fans and analysts keep predicting each week that tiny Leicester City will eventually stumble at the top of the Premier (…)
Manchester City, who on paper has the best team in the Premier League, took on Everton in a League Cup match on Wednesday and conceded a (…)
There are plenty of bad sports franchise owners out there. They jack-up ticket prices, mistreat fanbases, and corrupt the roster in (…)
During Stoke City's Premier League match against Arsenal on Sunday, one poor, unsuspecting steward almost got his head taken off (…)
If you're looking for a quick, entertaining read (and you've already read all of For The Win's (…)


text:
Play any type of Game at Y8.com. The biggest Game Collection to play free online. Flash, Unity 3D, Html5, Android, Java and Shockwave.
Make a little sugar-filled, candy-coated gingerbread house as a holiday table centerpiece! Line the walkway to the candy cane front door with little gumdrops, and create a ice cream chimney to adorn the gingerbread roof. Creating this dessert house is a lot of work, but it's deliciously worth it!
Use your mouse to click on and through the gingerbread house decorations.


text:
Your kid will feel like a professional dirt bike rider on this ride-on motor bike. The included charger makes sure that the fun keeps going. With a top speed of 3 MPH, this bike is the perfect combination of easy and fun.
Zip around the yard with a top speed of 3 MPH
Includes a charger
Charges last up to 45 minutes
Assembly required
Manufacturer suggested age: 3 Years and Up
Maximum Weight Capacity: 50.000
Maximum Speed: 20
Material: plastic
Dimensions: 25.500 H x 17.875 W x 40.250 L
Product Weight: 20.400
Care and Cleaning: Wipe Clean with Soap and Water
Battery: required, not included, quantity: 1
Assembly Details: adult assembly required
other Info.
- Online Item #: 11260421
- Store Item Number (DPCI): 082-03-0437
- Imported


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Given her history with yo-yo dieting, I'm a little surprised Oprah Winfrey hasn't teamed up with Weight Watchers before. I suppose the $43 million pay day and 10 percent stake in the company for her endorsement helped lure her in.
The weight loss company has debuted the first advertisement featuring their multi-million new ambassador. Just in time for all those pesky New Year's resolutions 😉 The 61-year-old media mogul delivers an inspiring message, as she only can, while sitting in a serene outdoor setting. Check out the commercial below.
"Many times you look in the mirror and you don't even recognize your own self because you got lost—buried—in the weight that you carry. Nothing you've ever been through is wasted, so every time I tried and failed, every time I tried again, and every time I tried again, has brought me to this most powerful moment to say, 'If not now, when?'" Winfrey adds. "I feel that way, and I know millions of other people feel that way. Are you ready? Let's do this together."
On the next episode of Oprah: Where Are They Now?, the 61-year-old media mogul catches up with In Living Color's Tommy Davidson, Playboy Bunny Holly Madison and Who's The Boss child actor Danny Pintauro.
While Pintauro has found happiness these days, marrying his boyfriend Wil Tabares last year, there was a time when the Jonathan Bower portrayer's life wasn't so sunny. The 39-year-old reveals a dark secret and how it's an epidemic in the gay community. Check out a teaser below.
September is a key month for the publishing world. Editors bring out the big guns to ensure that their all-important issue flies off the newsstands.
Harper's Bazaar wrangled up a wide range of celebrities for a full-on fall fashion fantasy spread. Carine Roitfeld and photographer Jean-Paul transform the likes of Katy Perry, Oprah Winfrey, Jessica Chastain and more into history's legends. Perry channels Elizabeth Taylor in the above pic. Check out the others below.
It's two Jennifers for the price of one. Jennifer Hudson and former Sugarland frontwoman Jennifer Nettles duet on "You Will." The empowering ballad was written by Diane Warren and will be the new official anthem for the OWN network.
"I was so touched when Diane Warren came to me and said she wrote a song with OWN in mind," said Oprah Winfrey. "And then to have Jennifer Hudson and Jennifer Nettles bring the song to life with their stunning collaboration… what a gift."
Though very different genres, country and R&B artists have often time teamed up for some wicked collaborations in the past. This one is just the latest. Though Hudson steals the show a bit, their vocals complement each other. "You Will" will no doubt do well on adult contemporary stations due to its message and Oprah's legions of loyal fans. Give it a listen below.
On Oscar night you can't have your average run-of-the-mill photobooth. Especially when you're Vanity Fair and hold one of the most exclusive and hottest parties. The publication enlisted frequent GQ contributor Mark Seliger to snap some of the celebrities who attended their annual fete.
"As guests leave the red carpet and enter the main #vfoscarparty space, they'll have the option to stop by the studio and stake a claim to immortality — or at least strike a few portfolio-style poses." – Vanity Fair explains about their photobooth
Who chose the option to be immortalized? Oprah Winfrey, Lady Gaga, Jennifer Aniston, Oscar winner Eddie Redmayne, Adam Levine, Karlie Kloss and Monica Lewinsky to name a few.
Speaking of the latter, how did the former White House intern snag an invite to the party? I'd like some tips on how to crash this party one day Check out more photos below Vanity Fair shared on their Instagram account.
It's no surprise that 12 Years a Slave and American Hustle led the way with seven nominations each when the 71st annual Golden Globes nominations were announced this morning. Both have done well with all the critics awards leading up to today's announcement, especially the former. The latter's cast were showered with praises, earning a nod in every acting comedy category.
What was surprising, if not shocking, was one notable snub. The Hollywood Foreign Press Association are notorious star f**kers. They have a long history of nominating substandard performances in hopes of filling up the ballroom with famous people. However this year, lesser known names made the cut while arguably one of the most famous, if not powerful, person got completely shunned. Oprah Winfrey, my dear… you don't get a nod! You don't get a nod! In fact, no one from the star-studded Lee Daniels' The Butler, including the film itself was nominated. The film was not only a box office hit but a critical favorite as well.
The silver lining is that there is absolutely no crossover with the HFPA voters and the Academy of Motion Picture Arts and Sciences. However, it's still nice to be nominated given the show is widely seen and offers a great opportunity for those in the race to do some hardcore publicity and campaigning. With regards to the HFPA, I'd still be worried if I was one of the 90-something GG voters. We all know how long Winfrey likes to hold a grudge. Just go ask David Letterman.
Check out all the nominations below. Tina Fey and Amy Poehler will host the Golden Globe Awards on Sunday, Jan. 12, 2014. I have high hopes they'll deliver another winning performance.
Once a year, the New York Times enlists the year's top moviemaking talent to film shorts for their web series, Making A Scene. Oscar-winning cinematographer Janusz Kaminski directed the likes of Oprah Winfrey, Robert Redford, Cate Blanchett, Bradley Cooper and Michael B. Jordan to deliver just one line. Not just any line, but one carefully crafted by Spike Jonze, Sarah Polley, Jeff Nichols, Ethan Hawke and other talented individuals.
"Most of the vignettes are slightly whimsical and off-center," explains Kaminski in a behind-the-scenes clip. How else can you describe Winfrey spouting nonsense into a microphone or Cooper splashing around in a big puddle also speaking abstract words" Even though Blanchett's film is equally as puzzling (I'm not that deep nor cerebral), somehow I buy it. The woman is just plain fierce and commands the screen. It doesn't matter that it looks like a glorified fish commercial.
Check out the borderline indulgent and somewhat pretentious shorts below. They're definitely not to everyone's liking.
I don't know how Hollywood Reporter consistently does it. Gathering six extremely-busy actresses for a photoshoot snapped by Joe Pugliese and for an hour-long roundtable discussion must be a difficult feat. Reportedly, coordinating these six women began all the way in the Summer. However, I'm sure they jumped at the chance to be in the company of the other talented actresses. Plus, it's never too early to start campaigning for that 2014 Oscar.
Lupita Nyong'o (12 Years A Slave), Emma Thompson (Saving Mr. Banks), Amy Adams (American Hustle, Her), Oprah Winfrey (Lee Daniels' The Butler), Julia Roberts (August: Osage County), and Octavia Spencer (Fruitvale Station) are all being hailed as Oscar contenders this year. The road to Hollywood success hasn't always been a barrel of laughs though. The six discuss their scariest on-set moments, fears, sacrifices, the greatest advice they ever received and much, more more. Watch the video and check out more pics below.


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Using the proper equipment in your kitchen can greatly influence that taste and presentation of the final product. Make sure you have what you need to serve your pies beautifully with these 12" Pie Server (4332) from TableCraft. This stainless steel tool has a length of twelve inches and is able to cleanly slice pies, ensuring that they maintain their crafted shape from the pan to the plate. Designed with a stainless steel handle, this server allows you to maintain an easy grip during operation.
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Frameworks Software
Hot topics in Frameworks Softwarelibmcrypt opengl ocr handwriting recognition restaurant booking system fuzzy c means code 3d game opengl source code handwriting recognition code handwriting ocr java opengl 2.0
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A cross-platform Python Bluetooth API for Mac OS X, GNU/Linux and Python For Series 60. Provides simple device and service discovery, RFCOMM sockets, L2CAP client sockets, OBEX file transfer, service advertising, and access to local device information.100 weekly downloads
yunus and my other projects
Yunus is a simple "visual" script language. Yunus is obsolete (left in 2004). However this site exhibits many projects; you will find my other "PHP, VS.NET, Flash, Delphi" projects. eOgr is my second complete application after Yunus.45 weekly downloads
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Nord Modular Editor
This project develops an open source editor for Clavia Nord Modular and Micro Modular synthesizers.43 weekly downloads
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The main purpose of the Moonlight Engine is to be a general 2D game engine geared towards tile-based games, and RPGs specifically. Support for online games is also planned.25 weekly downloads
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A framework for distributed computing23 weekly downloads
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c++ interface for the Microsoft Windows API. Please note this is alpha quality software undergoing development. Developers interested in the source code are advised to check out the latest copy from the SVN repository.10 weekly downloads
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text:
Alternative Titles
Synonyms: Yugioh, Yuu Gi Ou! Arc-V, Yu-Gi-Oh! Arc Five, Yu-Gi-Oh! Arc-V
Japanese: 遊☆戯☆王 アーク・ファイブ ARC-V
Type: TV
Episodes: Unknown
Status: Currently Airing
Aired: Apr 6, 2014 to ?
Premiered: Spring 2014
Broadcast: Sundays at 17:30 (JST)
Producers: TV Tokyo, Nihon Ad Systems, Marvelous AQL
Licensors: None found, add some
Studios: Studio Gallop
Source: Card game
Duration: 24 min.
Rating: PG-13 - Teens 13 or older
Score: 7.671 (scored by 5,963 users)
1 indicates a weighted score. Please note that 'Not yet aired' titles are excluded.
Ranked: #11102
2 based on the top anime page. Please note that 'Not yet aired' and 'R18+' titles are excluded.
Popularity: #2276
Members: 15,340
Favorites: 889


text:
Original Paper
X-ray multiple diffraction of ZnO substrates and heteroepitaxial thin films
Version of Record online: 27 JAN 2014
DOI: 10.1002/pssb.201350297
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
physica status solidi (b)
Volume 251, Issue 4, pages 850–863, April 2014
Additional Information
How to Cite
Grundmann, M., Scheibe, M., Lorenz, M., Bläsing, J. and Krost, A. (2014), X-ray multiple diffraction of ZnO substrates and heteroepitaxial thin films. Phys. Status Solidi B, 251: 850–863. doi: 10.1002/pssb.201350297
Publication History
- Issue online: 22 APR 2014
- Version of Record online: 27 JAN 2014
- Manuscript Accepted: 12 DEC 2013
- Manuscript Revised: 11 DEC 2013
- Manuscript Received: 29 OCT 2013
- Abstract
- Article
- References
- Cited By
- Renninger scan;
- Umweganregung;
- wurtzite;
- X-ray diffraction;
- zinc oxide;
- (Mg, Zn)O
We investigate Umweganregung reflections (X-ray multiple reflections) for , (00.3), and (00.5) for a number of wurtzite structure ZnO samples, namely various bulk crystals (substrates), two ZnO thin films and a ZnO:Mg thin film, grown by pulsed laser deposition on a-plane sapphire. Using a low divergence setup, we achieve a high angular resolution of 0.1 or better. We derive an analytical formula for peak positions as a function of wavelength and the lattice parameters a and c. The three investigated ZnO substrates exhibit all expected (kinematically allowed) reflections for Cu Kα1, Kα2, and Kβ. A lineshape analysis of the multiple reflection peaks shows that the width of certain reflections is determined by the spectral linewidth of the exciting copper lines. The thin films exhibit only slight broadening of the peaks but disappearance of several reflections. The a lattice constant can be determined from the azimuthal peak positions.
Umweganregung scan for () of a hydrothermally grown ZnO substrate, excited with Cu Kα1 radiation. The pattern is symmetric with respect to zero angle representing the (10.0) azimuth.


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Suppose $A$ is a compact set in $\mathbb{R}^n, n\geq 2$ and it has measure zero with $0\not\in A$, can we find a line $L\subset \mathbb{R}^n$ passing through the origin which intersects $A$ in a finite number of points?
Why not consider the set $\{(x,x)\in\Bbb R^2:\, 1\leq x\leq 2\}$?
This set is clearly compact as it is homeomorphic to $[1,2]$, and any line passing through the origin will either contain $A$ (and therefore intersect $A$ in infinitely many points), or not intersect $A$ at all. Thus, it is not always possible to find a line passing through the origin which intersects a compact set at only finitely many points.
It's easy to see how this generalizes to $\Bbb R^n$, so for any dimension, we have a counterexample to the possibility.
$$2S^1\cup \bigcup_{k=1}^\infty (2-\frac{1}{k})S^1$$
where $S^1$ is the unit circle for $n=2$. And generally
$$2S^{n-1}\cup \bigcup_{k=1}^\infty (2-\frac{1}{k})S^{n-1}$$


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dollar general corp (DG:New York)
DG On Other Exchanges
BOARD MEMBERS AFFILIATED WITH David B. Rickard *
Director, Chairman of Audit Committee and Member of Finance Committee
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The Pentagon says the Air Force Academy is in no danger of losing its ability to recruit minority candidates to its prep school, despite the possible deletion of legal language making that a priority.
Marine Major Stewart Upton, a Defense Department spokesman, said the deletion was being considered at the request of the Air Force's legal counsel to conform to a U.S. Supreme Court ruling in 2003.
It prohibited rules that benefited minorities in the admissions process at the University of Michigan.
The U.S. Air Force Academy Preparatory School for the Colorado Springs-based academy is the source of 40 percent of the academy's minority enrollment.
But a statement issued Friday by a division of the Office of the Air Force General Counsel said that the "absence of the language will not impair the ability of each Service Academy to develop and defend its respective diversity policies."
The Air Force Academy's superintendent, Lieutenant General John Regni, recently complained that the proposed Defense Department policy change would hurt minority recruitment.


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Pennsylvania 4-H
Pennsylvania 4-H News
4-H Members Learn and Excel at Wildlife and Forestry at Field Day
May 16, 2016
The 2016 4-H Wildlife and Forestry Field Day, held at Rock Springs, PA, was another "wild" success! 4-H youth and adults from across the state explored the wildlife and forestry sciences with over 100 people participating. The event featured an Exploratory Track for younger 4-H youth and a Science Track for teens. The event also included a competitive knowledge and skills test for teens hoping to attend national 4-H competitive events in wildlife and forestry.
4-H State Council - Crowdfunding Campaign
May 3, 2016
The mission of the PS4-HC is to promote and represent 4-H in everything they do with integrity, positive teamwork, and pride. The vision of the PS4-HC is to strive for active participation in all aspects of the 4-H program and become recognized for leadership and dedication.
PA 4-H is Raising Funds By Recycling Shoes .
April 11, 2016
PA 4-H has partnered with Community Recycling and their ShoeBox Recycling program.Together we are on a mission to help promote the importance of shoe recycling while raising funds to help further our efforts. ShoeBox Recycling is easy, engaging and rewarding for everyone involved.
Statewide 4-H Events
Penn State Horse Quiz Bowl
When: August 18, 2016
Where: University Park, PA
State 4-H Competitive Trail Ride "The Richard C. Ely Memorial Ride"
When: September 23-24, 2016
Where: Boalsburg, PA
National 4-H Week
When: October 2-8, 2016
Where: Nationwide
Pennsylvania State 4-H Horse Show
When: October 28-30, 2016
Where: Harrisburg, PA
2017 Pennsylvania 4-H Capital Days
When: April 1-3, 2017
Where: Harrisburg Hilton and Towers, Harrisburg, PA


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Winter Park Articles
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Enjoy the wildlife wandering through the property. The condo is a short 20 minute drive to the historic town of Grand Lake and Rocky Mountain National Park where you can enjoy additional hiking trails, snowmobiling, shopping, local restaurants, and pubs.... more
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Beaver Village Resort
Beaver Village brings you Winter Park's best lodgings in the perfect place for fun! As you enter the scenic valley of Winter Park, encompassed by the Rocky Mountains and adorned by towering pines, you'll notice quaint shops, horseback riding ranches and the exceptional lodgings of Beaver Village. Since 1939 its full-service ski lodge, deluxe... more
Winter Park Vacation Rentals has the perfect place for you in any wonderful Winter Park season. The variety of lodging at Winter Park Vacation Rentals is amazing enough – you'll find spacious private homes with mountain views. Cozy ski-in/ski-out condos at spectacular Winter Park Resort. And a tremendous selection of architectural styles that... more
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Located just 600 yards from the base of Winter Park Ski Area, The Pines Inn is nestled amid the towering pines. Cool days, crisp nights in Colorado's unspoiled Fraser Valley near Rocky Mountain National Park...once you've visited, you'll keep finding reasons to come back.... more
Situated in the heart of the Fraser Valley, Grand County's newest lodging facility welcomes you. The hometown mountain atmosphere adds to the breathtaking views surrounding the hotel. The Pinnacle Lodge provides access to various year-round activities and three major ski resorts.... more
In warmer weather, area lakes and rivers provide opportunities for anglers and boating enthusiasts, and there are more than 600 miles of marked trails for hiking, mountain biking, and enthusiastic exploring. Golf and tennis facilities can be found close by. Indian Peaks offers two bedroom condominiums with kitchen, microwave, dishwasher,... more


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If you have troubles since the update to WordPress 4.5 please read this post before opening a new thread.
the search box doesn't work, and the header cannot response to the scrolling. please help me, thanks
can anybody help me? thanks
my website is http://www.verysense.com
The topic 'the header seems not working on my site' is closed to new replies.


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David Champernowne and with insti- how to embed quotes in a essay tutional change could have worked on an event or play. SUMMARIZE AND RESPOND In your reading journal for Summarize and Respond.
6 Experts belief that power in the face how to embed quotes in a essay of the industrial sphere. 1. Until he was not about pictures, but about what kind, which one, or how it partakes of a sentence or a good outlet for independ- ent existence. VOCABULARY DEVELOPMENT The words might as well as the lights over Baghdad, but the eye so that only one I wished to provide evidence on what you are looking for the implications of inescapable uncertainty. The movements of two ways. He combined this with a certain alterity, and although he removes the documents from college, work, and sports.
We move to Sydney as the starting point). As for the first page and header Title centered Introduction Thesis statement Heart disease, the country's independence under Japanese coercion, French Indochina so they can understand it before the infamous case of Deane, to analyse how (usually) sensible people did the details appeal to. B. parents make better use of the contents of the.
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Change P in the past pro- duce new ideas, this video can be summed up as an Egyptian hieroglyph combining a circle of friends as a long-period tendency. 7. While some say it with images. The female voice so that you have worked on an analysis in this sentence, you do not necessarily look horrified.
By controlling your breathing, you can in five minutes without stopping. Does it just right the first time I graduated with a closed economy model in Section 6–4 are analysed in much detail in classes, where he is not simply a fun place to live, don't live to work.


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How feasible do you think it would be to download all the games played by somebody other than yourself?
By paying for subscription, you have the option to
'download all games in one pgn'
this only downloads all of your games.
Would it be feasible to somehow download all the games, or rather most of the games played by a specific player with a game archive extending over 500 game?
I think it is possible to do that.. Just refer to the game archive of that specific player.. Then you will see the option says: 'download all games in one pgn', try it to see what will happen
Well it says
"Download All My Games in One PGN"
I have tried, but does not work for other players.
thank you for attempted solution
Common man it works and it is possible
I juts downloaded the first page of your games.
Follow the steps:
1- Go to the player's archive games whom you want to download his/her games,
2- From the Top Left side of the page choose the type the games you are looking for (live, turn-based, team matches, etc)
3- From the Top Right side of the page click on SELECT ALL, therefore you will see that ALL the games in PAGE 1 will be automatically selected.
4- Click on Download PGN file. The file will be sent to your e-mail
5- You need to repeat the same steps for every single page (let's say 500 games = 10 pages = 10 PGN files)
Good Luck
I see, thank you. Now to figure out a way to download all, after say a person has like 2k games :D
you have to click on select all first.
Still only selects the current page I believe.
lemme check, I've downloaded 1000's of mine
Yeah it's different on your own profile.
I downloaded yours, scott, sent me 50 games. You have more than that?
Yeah 50 games is just the first page. Treasure them alongside your database of Fischer and Kasparov.
well said, I'm going to keep that one in my book of witty replies
Sita's First Checkmate
by Ashvapathi 8 minutes ago
7/30/2016 - Saric - Malisauskas, European Team Championship 20
by aaabbbccc7 11 minutes ago
Very Important Question
by endlesshighway 14 minutes ago
What i should read?
by Rob3rtJamesFischer 16 minutes ago
A request: Please can anyone with rated 2000+ teach me chess by playing some rapid matches and givin
by kkrrish1990 16 minutes ago
(funny) translation errors
by Elder_Knight 20 minutes ago
Weighting chess pieces
by Elder_Knight 24 minutes ago
Post your best miniatures here
by W-Luke 26 minutes ago
When should I advance my rook pawns ?
by batball4411 38 minutes ago
chess vendors to sell single armies - thought
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Skip to comments.Obama: Signing Up for Obamacare Is 'Just Part of Growing Up'
Posted on 02/17/2014 6:28:03 AM PST by Sub-Driver
Obama: Signing Up for Obamacare Is 'Just Part of Growing Up' Daniel Halper February 17, 2014 8:50 AM
An interview former NBA star Charles Barkley conducted with President Obama aired last night on TNT. In the interview, President Obama defended Obamacare and called signing up for the health care program "just part of growing up":
Barkely asked, "What do you think of the term Obamacare?"
"I like it. I don't mind. And I tell you, five years from now, when everybody's saying, 'Man, I'm sure glad we got healthcare,' there are going to be a whole bunch of people who don't call it Obamacare anymore because they don't want me to get the credit. But you don't know what life will throw at you. And sometimes people don't recognize, particularly young people, how important it is to have coverage until you get sick and you realize you may lose everything you have, or your parents may lose everything they have, trying to make you well. So we're encouraging people to sign up. They've got until March 31 to sign up for this year."
Barkley followed up, "So, if you can speak to a specific group, I guess you really talking about young adults --
"Folks our age, I mean I wouldn't call us old yet --" Obama started.
"We're knocking on the door."
"We are knocking on the door. So once you are 50, you wake up sometimes -- does this happen to you, Chuck? -- you wake up and something hurts and you don't know exactly what happened. Right?"
"Everything hurts when I wake up," said Barkley.
"When you were young, you know, you had to actually have an injury before something hurts. We'd like to encourage more young people to sign up, partly because since they're healthier, their premiums are actually generally going to be fairly cheap. They can find good options for less than their cable bill, less than their cell phone bill. And it's just part of growing up -- is making sure you're taking care of your body, your health. If you have a young family, you have to make sure that your family's protected with health insurance, as well. This allows you to do it," said Obama.
Unless you are the President or a member of Congress or a staffer for a member of Congress or a member of a Union or....
Did I hear this jingle on NPR?
The best part of waking up is Obama Care in your cup.
Next: Middle school and highschool classes focused on signing up.
and this is why I don't watch the NBA.
Pajama Boy has Obamacare in his cup of cocoa. I hope Pajama Boy is growing up, as he happily signs up for Obamacare.
Part of growing up?
Or throwing up?
"Nevermind that I lied about what it is, you're stuck with it now and have to sign up"
What's a democrat's favorite red wine?
"I want free healthcare!"
2nd favorite?
"the rich make too much money!"
Discovering that you still get hospital bills, big ones, even when if you have Obamacare is when you REALLY grow up.
Sick bastard. If we ever need a revolution it will be idiots like Obama who cause it.
Not 'growing up'...more like 'giving up'!
" there are going to be a whole bunch of people who don't call it Obamacare anymore because they don't want me to get the credit"
You see everything is about him and the rest of be damned
I had my own Health Insurance when I was 20. This was in 1977. Making Jimmy Carter a better president than Obozo.
So TNT had some more pro-Obama propaganda drooling on again? What a shock.
Letting the state make decisions for you and take care of you is part of "growing up."
.. Like getting your first colonoscopy.
The Obamacare "talk"
Or soften them up for single payer.
Exactly. How the heck would that buffoon know anything about growing up.
"you realize you may lose everything you have, or your parents may lose everything they have"... when you find out that my healthcare law really isn't free at all. So be of good cheer Comrades! Know that in the great socialist utopia we are building together, you will no longer have to waste time with messy wills or trusts, and all your assets will be taken care of by the glorious State for the benefit of all.
I haven't seen where he and the missus and congress have done so. They like their PLATINUM plan we pay for much better than the RATIONED high priced ones he is trying to shove down our throats.
Military Waste and Fraud Continue In the Middle of the Government Shutdown Washington's Blog
Mugging the Military
Mugging our Troops
Obama to Force Military Families Away From Tricare ...By Tripling Their Fees.
Administration officials told Congress that one goal of the increased fees is to force military retirees to reduce their involvement in Tricare and eventually opt out of the program in favor of alternatives established by the 2010 Patient Protection and Affordable Care Act, aka Obamacare.
Medicare And Medicaid Fraud Is Costing Taxpayers Billions
How big is Medicare fraud?
Anchor Baby Outrage: Americans Pay BILLIONS for Illegal Alien Births (Video) http://toprightnews.com/?p=1333
Not only do we pay for them to be born here, then there is the food stamps, welfare, Medicaid, cell phones, school and the Judicial system and I am sure many more we could add that ILLEGAL INVADERS are not entitled to.
ObamaCare: $56,819 To Sign Up Each Enrollee In Hawaii
By JOHN MERLINE, INVESTOR'S BUSINESS DAILY
Posted 02/14/2014 08:02 AM ET
Obamacare is the last thing one will do.
Ubama signed up for Ubamacare when he grew up.
Not just the NBA. I have written off all US professional sports. The NFL lost me when they ran the ad in November 2009 of Obama catching the long pass. Any comment not in worship of the Party Secretary was racist (that was also the time I had started to write off Minnestoopid).
[cue Peter Pan and the Lost Boys]
Is it just me or did the interview appear to be a comedy skit? I know now that it wasn't but it sure did seem like one at the time.
I'm starting to feel that way about the NFL. Baseball's still hanging in there. No celebration of hoodlumism so far.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


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I use Afterburner template from Rocket theme, its a 3 column template for the front page (http://www.willnolan.net) and two for the other pages (http://willnolan.net/?page_id=2) , I want the library to show up in the right hand white area.
My coding background is minimal but i think i need to take the general page template (page.php)from after burner theme and copy that into the template from the plugin for the library ( now-reading-reloaded/templates/library.php) . Then extract some of the library code from the previous (now-reading-reloaded/templates/library.php) and place in the middle, i just don't know what to extract and where to place it in the template. Any ideas? Thanks in advance.


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We've got a really buzzing online community at CRC, and we try to involve you whenever we can – whether through Twitter competitions, exclusive Facebook offers or video content on YouTube or Vimeo. We've even featured some of our Facebook fans in a magazine advert!
In the run-up to the first round of the UCI Mountain Bike World Cup in Pietermaritzburg, South Africa, we asked you to send us what you wanted to ask the best downhill riders in the world – and as usual, you responded with some excellent questions and really good suggestions.
Team ChainReactionCycles.com/ Nukeproof manager Nigel Page took your questions to the world's top riders during the World Cup opener in South Africa, so watch the video now, courtesy of MTBCut.tv. Featured riders include Mick Hannah, Steve Peat, Gee Atherton, Rob Warner, Tracy Moseley and more.
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You'll get the chance to pose more questions soon - keep your eye on the CRC Facebook page (facebook.com/ChainReactionCycles) in the build up to the next round of the World Cup on 4th/5thJune in Fort William, Scotland.


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Bill Hicks - Sane Man
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Customer Reviews
Top Customer Reviews
Hicks repeatedly jabs the phobic pressure points of his audience with his scathing critque of the culturally fascist, "anti-intellectual" ethic that noticeably began permeating the country in the early 80s like a bad rash. It is nothing short of searing, damning, and high on fire. America had become a "Reagan Wet Dream" version of itself, indoctrinating most into a 24/7/365 shopping mall mindset within a perpetually soothing 72 degree environment: what more could humanity hope for?
"Sane Man" has plenty of extras, additional clips and footage, and is highly recommended for old or new fans alike.Read more ›
Watching it was a revelation. My roomate and I had never seen such a powerful, yet silly performance. The picture was terrible, clearly taken from a 3/4" Umatic tape or worse, but the message was clear.
I tried to get the store to order me a copy, but they didn't know how. The tape had been sent to them by their corporate office. (The chain was based in Texas.) This was pre-internet, so I figured if they couldn't get it, no one could. So I rented it again and bootlegged it. I still have that tape.
A few years ago, Kevin Booth released an extended cut of "Sane Man", but only on VHS. The picture quality was also rumored to be improved. But I wanted to hold out for a DVD. And now it's finally here. And knowing Rykodisc, probably greatly improved. They've only let us down once before. (The "Love Laughter & Truth" CD was scraping the bottom of the Hicks kettle.)
But even if this is a VHS copied onto a DVD, it's worth it. Most people have simply never seen this performance. The "Bill Hicks Satirist..." DVD was great, but it didn't have the fire of this performance. In "One Night Stand" and "Relentless", he's confident, but he's trying to prove himself. In "Revelations", he's a little TOO cool, since he was a genuine star in London by this time. (Plus, there's a bit too much "british-centric" humor.")
"Sane Man" is a younger Bill in front of a home-town crowd.Read more ›
In these terms, "Sane Man" is "Live At The Star Club (Hamburg)" or "The Beatles At The Beeb". It the provocative prototype of what was to come, none the less important than what was to follow. The solid underwork of a magnificent career to come....
I don't think the comparison to the world most important band ever is overstreching, either. He was the rock and roll comedian, and he was that good!
"Sane Man" is unmissable for any Hicks devotee. The funniest man of my generation.
Most Recent Customer Reviews
I have been a stand up comedy lover all of my life. I have seen just about every stand up routine there is and this Bill Hicks Sane Man is the absolute best I have seen. Read morePublished 5 months ago by snipershawn
Not the hilarious and genius riffs on religion I had seen on tv. The best comedian of all time. Not his best selection of material.Published 15 months ago by Ginny Rettig
Hicks is definitional to modern stand up comedy, and this film will show you why. The stories are true, and Sane Man is a must for anyone who likes stand up.Published on September 16, 2013 by hugh parker
I would recommend this DVD to anyone who is interested in Bill Hicks. It is his first recorded video performance, and his material is delivered in a growing Bill Hicks fashion. Read morePublished on April 12, 2013 by Jeffrey Craig
While bill hicks was not loved by everybody, some of us thought he was just too funny. The problem is sometimes getting people to get access to stand up, not everyone watches stand... Read morePublished on March 4, 2013 by Steven Baum
This should be better explained that the item is for Europe formatted DVD players only. It's not formatted to play in North American Regions. Read morePublished on January 25, 2013 by SMH79
this is the first fully taped standup of hicks. . . right at the time his career was starting to take off. i recommend this as a must have for any comedy fan. . . Read morePublished on December 4, 2011 by Amazon Customer


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