text:
|Publication number
|Publication type
|Application number
|Publication date
|Jan 6, 1981
|Filing date
|Apr 24, 1978
|Priority date
|Apr 24, 1978
|Publication number
|Thomas J. Ingalz
|Original Assignee
|International Diagnostic Technology
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (5), Referenced by (13), Classifications (12), Legal Events (6)
|External Links: USPTO, USPTO Assignment, Espacenet
This is a continuation application of U.S. application Ser. No. 703,578 filed July 8, 1976, which is now abandoned.
Reference is made to a patent application in the name of Richard A. Harte, entitled "Fluorometric System, Method and Test Article", Ser. No. 703,579, filed simultaneously herewith, now U.S. Pat. No. 4,056,724, and Ser. No. 627,941, filed Nov. 3, 1975, now U.S. Pat. No. 3,999,948.
This invention relates to a photoluminescent system for the detection of sample substances, as derived from biological fluids or tissue labelled with photoluminescent materials and to fluorometers adapted for accurate measure of such labelled samples on a support surface of a carrier member.
There are many techniques available for the detection of an unknown quantity of a biologically derived sample (e.g., serum or urine). During such techniques, a labelled substance which has reacted with the sample must be separated from the unreacted labelled substances which includes free and non-specifically bound substance. This separation in liquid form is known to be inefficient, unreliable, and tedious. Many solutions have been proposed to solve this problem by the use of diagnostic reagents coated on a solid surface which combine with the labelled substance.
In one technique, reagents are coated upon plastic test tubes by physical adsorption of antibodies specific to the sample substance to be tested. See, e.g., article by Catt et al in the Journal of Biochemistry, 1966, Vol. 100, page 31c and in Science, Vol. 158, page 1570, 1967. This technique is difficult to control because of the non-uniformity of the plastic surface and imprecision in the coating technique. Furthermore, during washing to remove unreacted labelled substance, a relatively weak physical coating bond holding the antibodies can be disrupted resulting in their loss along with reacted labelled substance. Also, this technique requires a separate procedure for the coating of each test tube. This would be particularly time consuming, especially to insure reproducibility, if covalent attachment were employed to prevent the loss of diagnostic reagent. In addition, such test tube coating does not lend itself to the precise viewing required in a solid front surface fluorometric system. Furthermore, use of a test tube restricts covalent attachment to the material of construction used in making the tube.
Another solid surface approach is set forth in Bratu, Jr., et al U.S. Pat. No. 3,826,619. This system employs a physically adsorbed diagnostic reagent coated on the tip of a holder. This tip is first fitted into a receptacle for the sample and then into a receptacle for the labelled substance. This system is subject to inaccuracies because of losses in rubbing of the unprotected tip against the close fitting receptacle. Also, there is no ability to stir the tip in the receptacle leading to long incubation periods. Additionally, this technique does not lend itself to reproducible mass production as each holder must be individually coated with diagnostic reagent. Furthermore, it is undesirable for precise viewing.
An improved solid surface system is described in Deindoerfer et al patent application, Ser. No. 627,941, filed Nov. 3, 1975. In a preferred embodiment, a disc bearing a fluorescently-labelled diagnostic reagent is carried by an enlongate holder and inserted into a viewing housing of a fluorometer for precise positioning of the disc. However, the disc is not essentially isolated from the remainder of the fluorometer or the environment. Therefore, if the fluorescent label on the disc is read while coated with a liquid layer, it is subject to evaporative cooling. As it is known that the intensity of a fluorescent signal is dependent on temperature, such cooling would cause a drift in signal. If the evaporation is sufficient to cause the liquid layer to become discontinuous, this produces an additional source of signal drift based upon a modification of the optical characteristics of the surface. The advantages of reading the surface while wet are set forth in the copending Bolz et al patent application, Ser. No. 690,975, entitled "Method for Reading a Wet Fluorescent Surface", filed May 28, 1976, now U.S. Pat. No. 4,025,310, and are summarized hereinafter.
The apparatus of the present invention analyzes a sample by photoluminescent techniques. The sample is supported as an exposed layer by a surface portion of an elongate carrier member. The carrier member is inserted into a quiescent enclosure substantially isolated from atmospheric conditions and preferably light-tight. The apparatus includes an optical system for delivering excitation radiation to the exposed side of the sample surface and a photoluminescent collector which receives the illumination emitted from the same exposed side. The surface is read while coated with an aqueous film. The quiescent enclosure about the carrier member during reading minimizes evaporation of the aqueous film.
It is an object of the invention to provide an apparatus particularly adapted for precisely positioning the sample containing carrier member for viewing by a fluorometer.
It is another object of the invention to provide an apparatus of the foregoing type in which the sample surface of the carrier member is substantially isolated from ambient atmospheric conditions.
It is another object of the invention to provide an apparatus of the foregoing type which is substantially light-tight with the carrier member in an inserted position.
It is a further object of the invention to provide a method for reading a sample coated onto a carrier member and covered with a continuous aqueous film while enclosed in an apparatus of the foregoing type.
It is another object of the invention to maintain the sample surface in an isotherm condition to minimize drift.
Further objects and features of the invention will be apparent from the following description of the preferred embodiments taken in conjunction with the accompanying drawings.
FIG. 1 is a schematic cross-sectional view of a fluorometer constructed in accordance with the present invention shown with external portions in phantom lines.
FIG. 2 is an optical diagram of the fluorometer of FIG. 1.
FIG. 3 is a prospective view of a test stage assembly including a sample holder constructed in accordance with the present invention and particularly adapted for use in the fluorometer of FIG. 1.
FIG. 4 is a cross-sectional view, in elevation, of the test stage assembly of FIG. 3.
FIG. 5 is a cross-sectional view taken along the line 5--5 of FIG. 4.
FIG. 6 is a top plan view of the sample holder of FIG. 2.
The present invention relates to a photoluminescence detection system and method to quantitatively detect and measure a photoluminescent sample substance coated as a layer on a surface of a carrier member. The term "photoluminescence" includes within its scope fluorescence and phosphorescence. A fluorometer is employed to measure both types of emissions. As defined herein, the term "photoluminescent labelled sample substance" is one which includes a material derived from either a biological fluid or tissue and which, alone, or in combination with other materials, emits photoluminescence as a result of excitation with a selected wavelength of light in a solid layer form.
Referring to FIG. 1, there is shown such a lens type fluorometer, the overall outward appearance of which is shown in phantom lines. The optical system of the fluorometer is developed within an opaque block 104 of plastic, as for example, Delrin, selected because of its dimensional and thermal stability. Also, Delrin has low thermal conductivity and so provides excellent thermal insulation to the stage surrounding the sample surface to maintain it in an isothermal condition to minimize fluorescent drift due to temperature variation. The block is machined to accept the various components as shown in the drawings. The details of the machining are not believed essential to understanding the invention and, accordingly, they have been omitted for the sake of clarity.
Referring simultaneously to FIGS. 1 and 2, the system generally consists of three major optical systems: an illumination optical system 105 for supplying excitation light to the sample; a collection optical system 106 for receiving fluorescent output from the sample; and a reference level optical system 107 for periodically establishing and checking zero and preset intensity levels.
The illumination optical system 105 consists of lamp 110, condensing lens 111, excitation filter 112, chopper 113, and its lens system 114a and 114b, as driven by an electric synchronous motor 115 and focusing lens 116 for imaging excitation light onto a test stage 150 including sample 118.
The collection optical system 106 consists of a collecting lens 120, emission filter 121, and photodetector lens 123 which images the sample member surface onto a photodetector 124.
The reference level optical system 107 consists of a beam-splitter 130, a turning mirror 131, chopper 113 and associated reference lens systems 133, a diffusing screen 134, a portion of which is developed by lens 135 and re-inserted at beam splitter 136 into the collection optical system 106. Rotation of the chopper 113 causes light to pass alternately through the illumination optical system 105 or through the reference optical system 107. Thus the output at the detector is an alternating signal during one period of which the intensity of the excitation source is measured while the other period measures the fluorescent output.
Many of the optical components shown and described herein could be selected from a wide variety of available more complex designs, but the following have been found to be particularly satisfactory for use in the present invention. All of the lenses are of aspheric type and are standard products of Mells Griot, product 01 LAG 005. These lenses have f/0.75 and are planar on the non-aspheric side. Lens 120 which collects the light from the sample station is the only lens for which a substitution is not readily available in view of the large aperture and solid angle of collection required of that lens.
FIG. 2 contains ray tracing lines thereon which serve to illustrate the functions of the lenses and optical components. Thus, lens 111 collimates the light from the light source and passes it in parallel rays through the first filter at 112 after which the beam-splitter 130 divides off a certain portion of the light to be delivered to the reference optical system 107. The remainder passes to the chopper lens pair 114 in the excitation optical system 105. The first lens of this pair 114a focuses the light down to a small spot so that the cut-off and cut-on times and general level of intensity of light exiting from the chopper is well-defined in value over an appropriate period of time. The second lens 114b of that pair collimates the light for delivery to a focusing lens 116 which brings an image of the filament down into a small focused spot on a central area of the sample carrier 118.
The output from the sample is collected by planospheric lens 120 over a wide solid angle and delivered in parallel rays through the emission filter 121, through a beam insertion device 136, and thence focused by detector lens 123 onto the active element of the photodetector 124.
The portion of the beam which is removed for reference purposes is turned by turning mirror 131 to follow a path parallel to and alongside the excitation path. This light is passed through lens pair 133 which also brings the light source to a focus in the plane of the chopper so as to also provide for rapid turn-on, turn-off, and well-defined value of intensity when the reference beam is on. The reference beam is then passed in parallel rays to a diffusing screen 134 which can consist of a film of polyester, such as that made by DuPont (Type A), having a dull matte finish on the side facing the detection system 106. The matte finish serves as a light scattering function and thus converts the film into a secondary or reference source of high uniformity. A portion of the certain area of the film is taken by lens 135 and the rays made parallel for being passed to insertion mirror (partial) 136 lying in the collection stream path between the emission filter 121 and the photodetector focusing lens 123.
Referring now more particularly to FIGS. 3-5, the test stage 150 is shown in detail. This stage 150 is removable in its entirety to facilitate cleaning. The state 150 is inserted into a cylindrical passageway in block 104 at a lowermost position such that the carrier 118 containing the sample thereon faces upwardly and in a substantially horizontal plane within the assembly as a whole. By making this provision, together with certain structural features of the sample carrier, it is possible to insert and measure a sample having a substantially free liquid containing surface while simultaneously maintaining that surface free of changes during analysis (such as by evaporation). The stage is rotationally oriented for insertion into the optical block 104 by virtue of an elongate axial slot 151 formed along one side of the stage and adapted to accomodate a locating pin 153 mounted in the optical block. A similar locating pin 152 projecting in the elongate slot 151 indicates when the stage has been brought to a fully inserted position within the block.
The sample carrier 118 is mounted on a removable sample carrier member in the form of a spatula 154 (shown inserted in FIGS. 3 and 4 and separately in FIG. 6) consisting of an end portion 155 having a substantially planar surface 156 and an area of slight depression 157 formed at that end of the member on which carrier 118 is disposed to form a support for a film or coating of sample. The spatula 154 further consists of an elongate blade 158 having a registry projection 159 thereon and terminates at its other end in a paddle 160 suitable for being easily gripped by the operator. The spatula is easily removed and inserted into the stage, the latter normally remaining in place within the instrument. Thus, an elongate slot in the form of slot 162 is provided within the stage for receiving the carrier, together with an upwardly extending groove 163 which accomodates projection 159. When fully home, the parts appear as shown in FIG. 4. It will be further noted that the outward extremity of the mounting slot 162 (at 164) is provided with a converging wedge-shaped configuration so that when the spatula is fully seated, the end 166 thereof is urged downwardly against the floor of slot 162 into precisely positioned contact within the stage. With the utilization of plastic parts for many of the components, it is found preferable that these components be restrained laterally and in every other dimension so that any slight warpage of the plastic parts of which the carrier member is made is compensated for, the member being urged into exact position against the bottom of slot 162 within the analysis stage.
Consideration of the optical diagram with respect to the focusing of light onto the stage and to the carrier spatula and the collection of light therefrom as shown in FIG. 2 will explain the purpose for the relieved portions 168, 169 provided by the removed portions of the end of stage 150 and laterally located adjacent the sample 118. Once positioned, member 154 is maintained in position by virtue of urging contact made by a resiliently mounted setting member, spring-loaded ball 170, carried in the body 104 of the optical system and adapted to engage and urge the back inclined surface of projection 159 to urge the carrier member inwardly against slot 162 for precise positioning. In addition, ball 170 falls behind that back surface and assumes a position serving together with the projection itself to block light from passing through the slot 162. Such light is capable, if not blocked, of causing non-dark background readings.
An aperture 172 is provided through the stage and passes light from a small light-emitting diode 173 to a small light detector 174. This aperture is closed by the passing of the member 154 as the same is pushed into the unit and when closed provides a "ready" signal for the associated electronics. An additional aperture 176 extends from the region immediately below the sample carrying end of member 154 and in general alignment with the excitation light beam so that a photodetector 178 positioned immediately behind this second aperture indicates when the sample is actually in position and blocking light from the beam.
The importance of precise repeatable positioning of a fluorescent sample carried as a layer on a support surface of a diagnostic reagent holder is illustrated in Example 2 of co-pending application Ser. No. 627,941, filed Nov. 3, 1975. As illustrated there, even relatively minor differences of separation between the lens system and the surface to be measured cause significant differences in the measurement of the fluorescent signal.
It is a particular feature of the present invention that the test stage 150, even though removable together with its independently removable sample carrier member 154, nevertheless when assembled forms both a light-tight and air-tight enclosure within the optical assembly. The former eliminates background light; the latter retards evaporation from an aqueous film over the free sample surface at 118 during the measurement period. The latter generally quiescent enclosure is defined and formed by the carrier member 154 itself which closes aperture 176, by the stage and its close fitting relationship with the adjacent block, and by lens 116 and 120, both of which are sealed into contact within bores supporting the respective excitation and collection optical systems.
The intensity of a fluorescent signal on a surface is highly sensitive to the surface temperature. Thus, to prevent signal drift, it is important to maintain the sample surface in an isothermal condition during reading. This is accomplished by viewing the surface in a quiescent enclosure essentially isolated from ambient atmospheric conditions both within the apparatus external to the enclosure and also external to the instrument. The apparatus may be maintained within a few degrees of room temperature by a cooling fan in the apparatus external to the enclosure. Since the apparatus is formed of a material of low thermal conductivity, such as Delrin, such a small temperature differential is not transmitted to any significant extent into the quiescent enclosure of the test stage.
There are many advantages in reading a fluorescently labelled sample on a solid support surface carrier 118 of spatula 154 while the carrier is coated with a continuous aqueous layer or film. One is that a steady reading without drift is obtained so long as the surface is read prior to evaporation to discontinuity of the aqueous layer. In addition, the presence of the aqueous layer has been found to enhance the optical properties of the surface for transmitting a maximum signal. Furthermore, the last stage in an analytical fluorometric technique normally comprises washing the sample surface with an aqueous liquid. Thus, reading prior to complete drying of the surface speeds up the overall system. It is important to avoid evaporation of the aqueous layer to discontinuity as it is believed that such discontinuity is the cause of drift of the signal. The above factors are fully set forth in the aforementioned co-pending Bolz et al application Ser. No. 690,975, entitled "Method for Reading a Wet Fluorescent Surface", now U.S. Pat. No. 4,025,310.
A number of techniques are set forth in the last-named patent application for maintaining a continuous aqueous film during reading by a fluorometer, such as by reading in a horizontal position or by the use of a humectant. It has been found that the procedure of inserting the aqueous film containing sample support surface of the carrier member into a quiescent enclosure isolated from ambient atmospheric conditions external to the enclosure produces stable readings for significantly longer periods of time than such other techniques. It is believed that this is due to a marked decrease in evaporation of the aqueous film under such quiescent conditions.
Another advantage of reading the wet support surface of the present invention in a quiescent enclosure essentially isolated from the apparatus and the surrounding environment is to avoid evaporative cooling by convective air currents. As set forth above, the intensity of a fluorescent signal is inversely related to temperature. Thus, such evaporative cooling would create a source of upward drift in fluorescent signal.
A suitable technique for analyzing a sample label with a photoluminescent substance on carrier 118 is to deposit the labelled substance on the surface and to coat the same with a continuous aqueous film. Then, such surface end of the carrier member 154 is placed into stage 150 which, together with the illumination means and collection means, forms a quiescent enclosure around the sample. Excitation radiation is impinged upon the sample surface and collected at an optical photoluminescence collection end of the fluorometer while the surface is coated with an aqueous film. The surface is free of any liquid volume contained independently of the surface during reading (as in a test tube). It is apparent that the photoluminescence collection end of the fluorometer is spaced apart from the aqueous film.
When inserted, the elongate blade of spatula 154 serves to substantially fill elongate slot 162 due to close clearances. This not only creates the aforementioned quiescent conditions at the sample end of carrier 150 during reading, but also blocks light which would otherwise pass through the channel. Also, projection 159 from blade 158 blocks groove 163.
|Cited Patent
|Filing date
|Publication date
|Feb 23, 1965
|Dec 26, 1967
|Hazleton Lab Inc
|Bioluminescence reaction chamber
|Aug 28, 1974
|Feb 10, 1976
|Damon Corporation
|Apparatus for monitoring the rate of a chemical reaction
|Apr 12, 1976
|Mar 29, 1977
|Baxter Travenol Laboratories, Inc.
|Method and apparatus for delivering blood oxygen association curve information
|May 28, 1976
|May 24, 1977
|International Diagnostic Technology, Inc.
|Method for reading a wet fluorescent surface
|Title not available
|Citing Patent
|Filing date
|Publication date
|Jul 11, 1986
|Nov 1, 1988
|Murex Corporation
|Interactive medical laboratory specimen apparatus system
|Jul 25, 1989
|Apr 13, 1993
|Louis Thomas A
|Electro-optical measuring instruments
|Jan 27, 2005
|Feb 16, 2010
|Elan Vital (Uk) Limited
|Fluid analyser systems
|Mar 1, 2006
|Mar 16, 2010
|Aleksandr Ostrovsky
|System, method and apparatus for use in blood testing through luminescence
|Jan 26, 2010
|Nov 18, 2014
|Roche Diagnostics Operations, Inc.
|Orientation identification label, reagent container carrier structure, analyzer device and reader module
|Jan 29, 2004
|Aug 4, 2005
|Hoots John E.
|Method of tracing process printing fluids
|Mar 13, 2006
|Jul 13, 2006
|Hoots John E
|Method of tracing process printing fluids
|Mar 1, 2006
|Sep 6, 2007
|Gemoscan Canada, Inc.
|System, method and apparatus for use in blood testing through luminescence
|Jan 27, 2005
|Nov 8, 2007
|Moor Timothy N
|Fluid Analyser Systems
|Nov 10, 2009
|Mar 4, 2010
|Elan Vital (Uk) Limited
|Fluid analyzer systems
|Jan 26, 2010
|Jul 29, 2010
|Roche Diagnostics Operations, Inc.
|Orientation Identification Label, Reagent Container Carrier Structure, Analyzer Device And Reader Module
|Sep 17, 1999
|Apr 6, 2011
|Massachusetts Institute of Technology
|Biological applications of semiconductor nanocrystals
|Jan 27, 2005
|Aug 18, 2005
|Elan Vital (Uk) Limited
|Fluid analyser systems
|U.S. Classification
|356/244, 250/429, 356/246
|International Classification
|Cooperative Classification
|European Classification
|Mar 7, 1985
Owner name: WHITTAKER CORPORATION ,10880 WILSHIRE BOULEVARD, L
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:INTERNATIONAL DIAGNOSTIC TECHNOLOGY,INC.;REEL/FRAME:004390/0038
Effective date: 19850226
|Jun 22, 1989
Owner name: WHITTAKER CORPORATION, A CORP. OF DE.
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Owner name: WHITTAKER CORPORATION, A CORP. OF CA., CALIFORNIA
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|Feb 9, 1990
Owner name: SECURITY PACIFIC NATIONAL BANK
Free format text: SECURITY INTEREST;ASSIGNOR:WHITTAKER CORPORATION;REEL/FRAME:005311/0926
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|Mar 6, 1990
Owner name: WHITTAKER CORPORATION, A CORP. OF DE, DELAWARE
Free format text: MERGER;ASSIGNOR:WHITTAKER CORPORATION, A CORP. OF CA;REEL/FRAME:005268/0473
Effective date: 19861031
|Dec 16, 1991
Owner name: BIOWHITTAKER, INC. A CORPORATION OF DELAWARE
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:WHITTAKER CORPORATION, A CORP. OF DE;REEL/FRAME:005978/0016
Effective date: 19911029
Owner name: BIOWHITTAKER, INC., STATELESS
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:WHITTAKER CORPORATION, A CORP. OF DE;REEL/FRAME:005978/0016
Effective date: 19911029
|May 9, 1996
Owner name: WHITTAKER CORPORATION, CALIFORNIA
Free format text: RELEASE OF LIEN;ASSIGNOR:SECURITY PACIFIC NATIONAL BANK;REEL/FRAME:007815/0366
Effective date: 19900605


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One westbound lane of Interstate 16 remained closed early Thursday, a day after 27 cars and trucks collided in a fiery pileup. Georgia Department of Transportation officials said the right, westbound lane is expected to be closed until noon Thursday. The chain-reaction accidents around 8:10 a.m. Wednesday killed four people and sent nine others to a hospital.
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2003 Ford Mustang Mach 1 Premuim Mach 1 2dr Coupe (4.6L 8cyl 5M)
The re-issue of the Mach 1 Mustang is an excellent performance value for the money. This car has a tremendous amount of low end torque and easily runs the quarter mile in high 13 second range without any modifications, just as from the factory. The Mach has a distinctive muscle car exhaust note that is reminiscent of big block cars from the 60's. One drawback is the interior, which could have been done up with better quality. Fuel tank is relatively small (15 gallons) and requires frequent re-filling , especially if the car is driven hard.
Functional ram air hood scoop, 32 valve double overhead cam engine, body striping
Wider rear tires
This car has standard bad traits of "performance" cars that are normally forgivable - poor gas mileage, high insurance premiums, etc. Even the severely minimalist interior (ie., cheap and uninspired) was explained away as being for the "serious" performance enthusiast. Three years after purchase, I'm still waiting for my car to perform. It has no power in first gear - which is fairly important in city driving as you often don't get a chance to shift up before you're cut off. Gunning it to get high rpms looks kind of stupid in heavy traffic. This vehicle has also developed some suspicious rattles during its limited 4700 miles, including weird A/C noises. Maybe it's a lemon.
Suspension, handling & braking are fantastic - cornering in this car is a thrill. Nice stereo. Shaker hood. Exterior styling (although you have to be gentle with the black accents).
Remember that hp at 5600rpm isn't all that important if you never get to 5600 rpm. Spend a little time & money on a better interior. Better pipes! 4.9L should make some noise. And there was no excuse not to put a 6 speed in a car with a $29k MSRP - Example: Nissan 350Z.
What a blast to drive... This baby
handles and looks great. Real eye
catcher. Chicks love it!
Shaker hood scoop. Power Plant!
Dress up the interior better. Too
Great car! Not new to Mustangs but
this is the best so far. Interior is
getting old on design but is still
great. Exterior on the Mach 1 is just
awesome. Great performance. Great
bang for the buck overall! Excellent
engine. Awesome brakes! The seats
are the best in a Mustang yet. Highly
recommend this car for the muscle car
Shaker hood, 32V V-8 engine, great
I had issues with the A/C not
working. It was under warranty so FMC
fixed it. However, there was some
mumbling at the dealership about
seeing Mustangs coming back with
similar issues with the A/C. Go
figure. Gas mileage is poor, but you
don't drive this car to save gas.
Shaker hood scoop.
Shorter throw on the shifter.
More of a throaty sound from the
mufflers. Improve exterior/interior
components. The mirrors, cowl under
the wipers, door handles, interior
trimmings, etc. All appear to look
and have the feel of cheap plastic.
Im not a big fan of the Heritage rims.
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text:
Coleman Silvertone Pocket Watch
We don't know when or if this item will be back in stock.
Product Description
- Product Dimensions: 6 x 3 x 2.5 inches
- Shipping Weight: 1 pounds
- Date first available at Amazon.com: July 11, 2010
- Average Customer Review:
Product Specifications
Watch Information
|Brand, Seller, or Collection Name
|Part Number
Customer Reviews
Top Customer Reviews
The inside of the cover is like brushed steel, with circle patterns and the face looked nice and simple, with a second hand. The numbers were easy to read and included military time in smaller red font beneath it, as well as minute conversion in small black just above them. It made it easy to tell the time at a glance.
My only qualm is the chain isn't a little bit longer and doesn't have a replaceable clip at the end.
When you pop out to set the time, the minute hand flops around a teenie bit so when you set the time you have to be careful how you click the set knob back in or it can move the minute hand a little. Not very accurate and really annoying to me.
However, yes, it's fine for the price I guess but I'm now looking for another pocket watch. It's not worth it for me to send it back so I'm donating it to a thrift store and spending more money this time for something with a bit more quality.
It will keep time fine for a few days, then will go bad, I'll reset it, it will keep good time again for a few days, then it will go bad again. Can't find any rhyme or reason.
Replaced the battery, but that didn't solve the problem.
EDIT: finally got frustrated, bought another one. It soon started doing the same thing.
A watch should do one thing: keep accurate time. This one doesn't. If you need a shiny $20 paperweight that will sometimes tell the correct time, buy it. If not, forget it.
Sometimes the cheap turns out expensive.
If I could drop my review down to one star, I would.
It most certainly did. It's practical for me because I'm forever doing things with my hands that kill wrist watches, but the pocket watch is out of the way, safe in its namesake. Furthermore, nobody expects to see pocket watches anymore as cell phones are quickly putting even wrist watches out of style. It's fun to see the looks I get when somebody asks for the time.
This design is basic with no bells or whistes. Time and date aren't automatically set or reset at Daylight Savings Time and there's no satelite in the sky telling my little watch I've strayed to far into another time zone, it doesn't even tell me whether it's morning or night but for me that works. It is perfect for first time Pocket-watch owners who want to see if the idea will work for them without forking out a lot of cash or worrying if it'll get scratched up.
I've enjoyed owning this watch for practical, aesthetic, and entertaining purposes. In fact, this simple little number has made me quite enamored with pocket watches in general. When the current battery runs down I intend to try and get it replaced. If I cannot I plan to simply purchase an identical watch to act as the functional staple in my new collection.
(by the way; I've tried but it doesn't seem to work too good for hypnotizing people into giving you cash. Sorry folks!)
Most Recent Customer Reviews
I normally like almost every item with the Coleman name, this watch has been a big dissapointment. I ordered this watch because of the features on the face. Read morePublished on March 11, 2010 by A. Banks
I have to give this watch a "don't buy rating". If a watch doesn't keep accurate time its not worth any price unless you need a prop in a play. Read morePublished on July 4, 2009 by john strait
I had been looking for something simple and affordable.
It had been a good while since I had had a time piece, and I needed one that would get me to class on time. Read more
I have owned two of these watches. The first one ceased keeping accurate time after I had the battery replaced. And I mean wildly inaccurate. Read morePublished on March 28, 2009 by Lynn
This is one of my best buys EVER!!! Ican't believe how sleek this watch is. I was a little shaky buying this watch at first but now i'm soooooo happy i bought this. Read morePublished on December 24, 2008 by Rebecca A. Dodge
I get complimented wherever I go now that I have a spiffy new pocketwatch. Whether people comment on the uniqueness, the shinyness, the "retro" factor, or just how classy it... Read morePublished on August 29, 2008 by Marshall Hughes


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|Scrambled Eggs with Mayo
|Tweet Topic Started: Jun 25 2011, 02:13 AM (341 Views)
|Post #1 Jun 25 2011, 02:13 AM
I saw this in http://allrecipes.com
2 eggs
1 teaspoon mayonnaise or salad dressing
1 teaspoon water (optional)
1 teaspoon margarine or butter
salt and pepper to taste (optional)
In a cup or small bowl, whisk together the eggs, mayonnaise and water using a fork. Melt margarine in a skillet over low heat. Pour in the eggs, and stir constantly as they cook. Remove the eggs to a plate when they are set, but still moist. Do not over cook. Never add salt or pepper until eggs are on plate, but these are also good without.
Someone commented that you should whisk the mayo and water first. Another recommended to use Hellmanns Mayo. If you are in the Philippines I know that many mayo brands use sugar in their products so maybe adding mayo to scrambled eggs may not be such a sweet idea. You may want to look for unsweetened brands on the shelves of your supermarket.
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1995 Subaru Legacy Sedan L 4dr Sedan AWD
Bought at 98k miles, sold at 125k. This car had an oil leak when I bought it, a pretty impressive one where the fumes made me nauseous. After I got that fixed (oil pump resealed), and put on new struts and windshield, it was a good little car. In typical Subaru fashion, I could take it anywhere, do anything in winter. It would go through snow up past the bumper like a little submarine, and cling to the road like a booger. This was the only car I've had that, on a blizzardy day, traffic would be going nowhere near fast enough for me, and I'd be that guy over in the snowy lane, driving the speed limit on two or three inches of powder. The reason I sold this car was it seemed like it was developing some weird AWD issues. On turning the wheel to the max, it experienced that AWD hesitation/shimmy, but also occasionally it would do this ultra-weird pronounced lurching. No idea what that was, let the next guy figure it out (yes, I told him).
1995 Subaru Legacy Sedan LS 4dr Sedan AWD
Bought my 95 LS AWD wagon in 2007 w/ 117K. Needed a cheap car that I could carry liability only due to a DUI. This little beauty was a trade-in that I got off the back lot before it was detailed and pumped through the $$ machine they call the service department. I live in Central Oregon (Bend) home of the oldest Subaru dealer in the West (they say) Lot's of snow and mountains. I now have 189K on this 'temporary' car, 7 years later. Every so often I change the oil. Replaced alternator, battery, starter and a few light bulbs over the years. A bread truck backed into me and I now have a free car after getting a check for the damage that 'gives it character'. I do have a nice ML320, but
cup holder is a laugh.
I called my Subaru a Deathwagon for a GOOD reason- It could handle ANYTHING I put it through. I drove my ninja to the ground (rarely did repairs & oil) and he still kept going strong. The only thing that stopped him was being T-boned, which bent the frame, and insurance totaled him out. His body died at 18 years old, but the engine (about 185,000) is going strong in another Subie :) I drove 40mph in HARSH Utah ice &snow storms. While every other car was pulling over, my Subaru handled every slip and slide with grace. I can't explain what this fantastic vehicle and I went through, as I am a very aggressive driver- car chases, sidewalks, fields, bicyclists, long road trips, barriers, etc.
Never died. It sat for 2 years in a drive way, one day I threw in some new gas and it started right up! Even when it was T-boned and smoking, he kept driving. I'd run into a steel beam and he would keep going. Drove over railroad tiles/barriers and he kept going. Check engine light was on for 2 years straight, he still kept going! I could ALWAYS depend on this car.
I'm a female in my 20's, I know nothing of cars and never fix anything on them- we were the best combo!
I'm sure everything is taken care of in the newer Subaru. I will always go after a Subaru when purchasing vehicles again.
My boyfriend bought me a 1995 Subaru LS AWD Sedan. I was scared at first because it's an old car (about as old as I am) but it is amazing. It has prooven me wrong! It's great in the winter (especially needed in CNY)!
I have automatic start which is always a plus in the winter, and I love the AWD. Winters where I live can get pretty bad and I know this car won't let me down. It's perfect for a first car!
The ABS light is on, which I read is a problem with these cars. Have to get it looked at!
I bought my Subaru in April, 2009. It's very reliable and I am able to get anywhere I want, when I want. It drives great in the snow. It's built very solid, which most cars these days are not. I would recommend this car to any new drivers. It is a really tough car!
AWD, Reliability, Handling, Durability.
More power to the engine, comfortability, and don't place the cup holder right in front of the radio!
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