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Joan Huntington (I)
Joan Huntington was born on May 31, 1934 in the USA. She is an actress and writer, known for What Ever Happened to Aunt Alice? (1969), Gaily, Gaily (1969) and Hell Raiders (1968). See full bio »
Born:in USA
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My wife and I spend a morning in bed in our Hawaiian hotel room
When my wife and I got married in 1999, we went to Hawaii on our honeymoon. We stayed a few nights in Honolulu and then flew over to Maui for a week. It was a fantastic time and we vowed to go back for our five year anniversary. While there the first time, we were talking to another couple we had met in our hotel and talked about a resort on Maui that catered to a "sexual crowd." They told...Read On
Added: 18 Jul 2015 | Category: Wife Lovers |
Avg Score: 5 |
Words: 4,256 |
Tags: wife oral anal shower sex facial tit fuck cumshot
| 10 Comments
The message promised beer, cigarettes, and stimulating conversation - no strings attached.
Before Friends Shared Everything The glow from my monitor was the only light in the room. I sat, eyes transfixed, and silently pondered the message open before me. My heart raced, spurred on by the promise of a new adventure. Sure he seemed like a great guy, but at first glance they all do. Paula and I had found his stories first, reading them together and revelling in their raw...Read On
Added: 14 Nov 2014 | Category: Wife Lovers |
Avg Score: 4.88 |
Words: 2,358 |
Tags: mmf wife sharing exhibitionism bisexual oral sex
| 37 Comments
A special night and a special gift!!!
THE Night! A true tale from Castlequeen- "It's.....ready!!! Come see!!!" I step into the backyard and can't suppress the giggle. "What's so funny, Miss Smartypants?" he demands. What is so funny is our brand spanking new hot tub. Not the tub itself, but the setup surrounding it. Our plan is to install a low barrier surrounding it with lattice work that we'll grow ivy on so it's...Read On
Added: 27 Aug 2009 | Category: Wife Lovers |
Avg Score: 4.63 |
Words: 2,569 |
Tags: fucking husband and wife hot tub cum orgasm
| 3 Comments
A wife gives private show to her husband's buddy.
This takes place in the era before cell phones, the internet, etc. ************************ Mike had received a phone call from a good service buddy. They had not talked for some time. He had been in his home office upstairs and went downstairs to the family room where his wife Lynn was watching television. "Who was that honey?" "That was Chuck. He and I were best buddies when we...Read On
Added: 13 Feb 2015 | Category: Wife Lovers |
Avg Score: 4.86 |
Words: 4,330 |
Tags: wife infidelity cuckold voyeurism exhibitionism
| 9 Comments
Tis better to give than receive.....
They say women hit a sexual peak in their 30's. Having just turned 34, my husband of 10 years and I were enjoying a resurgence in our sex life with my insatiable new sexual appetite. I couldn't get enough of his cock and we spent every night trying new ways to please each other. After a month of nightly marathon sex sessions, my husband finally asked me for a threesome. He opened up after...Read On
Added: 26 Jan 2012 | Category: Wife Lovers |
Avg Score: 4.63 |
Words: 1,523 |
Tags: threesome bi-curious anal
| 3 Comments
A traveling musician's dream comes true.
Some years ago when I was Director of Music at a junior college on Long Island, I was leading a tour of some of the smaller towns in New England with our 30 piece orchestra. We traveled in one large bus and stayed with host families in the towns where we played our church hall concerts. One hot evening, after a long ride up the I-95, we arrived in Watervale, Maine, and made our way to the...Read On
Added: 06 May 2011 | Category: Wife Lovers |
Avg Score: 4.73 |
Words: 5,010 |
Tags: exhibitionism shaving filming strip tease straight sex masturbation oral sex
| 6 Comments
Wife goes to sex club with a girlfriend and shows her sexual freedom.
It was Amy and Mark's first real trip to Twist. They had gone once before, a few years earlier, but that trip was curiosity driven more then a trip to engage in anything. This visit was to fulfill one of Mark's fantasies. Twist was an adult club for couples who were either swingers or just wanting to explore different sexual avenues. It was also welcoming to singles looking for a 'no...Read On
Added: 19 Jan 2016 | Category: Wife Lovers |
Avg Score: 4.98 |
Words: 4,804 |
Tags: wife stranger sex club voyeur bareback
| 31 Comments
Part two of our first hotwife experience
We were just about to pour ourselves another glass of merlot when we heard a car pull into the drive and then the door shut and alarm being set. I looked at Karen with trepidation and rose just as the doorbell rang. I opened the door to be greeted with a huge smile and a strong handshake as Colin introduced himself. I ushered him in and led the way into the lounge. Karen was standing by the...Read On
Added: 04 Mar 2015 | Category: Wife Lovers |
Avg Score: 4.75 |
Words: 1,156 |
Tags: hotwife interracial first time hung
| 12 Comments
Thirty years ago my wife and I were living in the Dallas area and I had taken a new job as a sales representative. I had to attend training at the home office on the East Coast and was flying in to Dallas on Friday afternoon. My wife was picking me up at the airport and we were going to celebrate our first wedding anniversary. She met me in the terminal and was wearing a strapless sundress...Read On
Added: 09 Jun 2010 | Category: Wife Lovers |
Avg Score: 4.5 |
Words: 1,749 |
Tags: watching wife
| 5 Comments
Horny wife seduces lawn boy
You have the best looking lawn in the neighborhood. It may have something to do with the frequency that the young man who comes to mow, weed and fertilize. You love his strong muscular body, sweating in the sun as he works. And I'm sure he loves how you're always half dressed as you clean your husbands house. The look on his face was priceless the first time you offered him a drink of water...Read On
Added: 14 Sep 2011 | Category: Wife Lovers |
Avg Score: 4.53 |
Words: 1,467 |
Tags: horny wife handy man tease exhibitionist
| 2 Comments
My boyfriend Dan brings home a surprise for me one night and oh, what a surprise it was!
My boyfriend Dan and I always cook dinner together, bumping into each other and rubbing against each other "accidentally" in the kitchen as we prepare dinner. It's like the preview to the main attraction of bedtime activities to come later. Tonight, Dan is rubbing against me more than usual and he can't seem to keep his hands from copping a feel each and every time he comes near. Each time...Read On
Added: 28 May 2013 | Category: Wife Lovers |
Avg Score: 4.93 |
Words: 1,955 |
Tags: cuckold oral anal cum cock pussy
| 21 Comments
Second part of the true account of how my wife and I fulfilled our fantasy for threesome.
A knock at the door and I let Howard in. It had been 3 weeks since he had come round to take up where he left off all those years ago. An old flame that had never got beyond foreplay until just recently when Jan and I had agreed he would be the perfect candidate to see if we could indulge in a threesome, with her the centre of attention. Again Jan had set the rules of engagement and as...Read On
Added: 05 Jan 2012 | Category: Wife Lovers |
Avg Score: 4.79 |
Words: 1,443 |
Tags: threesome mmf
| 2 Comments
The riveting 2nd half to a wife's very loving 12 day Christmas marathon!
6. THE SIXTH DAY OF SEXMAS...Monday December 19 th , 2011 I woke up expecting some sort of hot kinky sex, but found the bed empty. I felt a surprising wave of disappointment to not see her in bed. I yawned and could hear music downstairs, so I grabbed my robe and made my way to the kitchen. I could smell the delicious aroma of bacon. Once I reached the kitchen my morning got even better. ...Read On
Added: 16 Dec 2011 | Category: Wife Lovers |
Avg Score: 4.98 |
Words: 8,243 |
Tags: stockings wife love romance anal voyeur
| 10 Comments
18 year old invited to learn the art of sex
Menage a Trois. It was just a couple weeks before my 18 th birthday. I had graduated from high school in June. Because of the poor job climate, I put a notice on the "Jobs Wanted" board outside a local market. "Hard working young man willing to mow lawns, clean garage, basement, and attic, pick-up truck for hauling trash, odd jobs. Reasonable Rates with guarantee of satisfaction!" along...Read On
Added: 23 Aug 2010 | Category: Wife Lovers |
Avg Score: 4.73 |
Words: 3,131 |
Tags: wifelover threeway instructing young man
| 4 Comments
My wife and I have a great night out after being away.
I was away overseas for a few months so when I came home I had planned a special night for the wife and I. I ended up booking us into a nice hotel downtown and got the in-laws to look after the kids. As part of the package, I had also planned a wonderful meal at a rooftop restaurant that overlooked the city. During dinner I gave my wife an expensive golden necklace that I had purchased for...Read On
Added: 05 Mar 2014 | Category: Wife Lovers |
Avg Score: 4.75 |
Words: 962 |
Tags: strip club exhibitionism blow-job voyeur
| 4 Comments
Wife's husband sets up the start of her sexual adventure with a trip to Las Vegas
Andee carefully removed the letter from the envelope. She had just come home from work to find it placed on her pillow, plainly marked "Just For You." She knew it was from her husband, as he had departed on his business trip earlier that day. And, as he often did, he had some scheme cooked up to add a little excitement to her life. This time the plan was for her to travel to meet him at...Read On
Added: 24 Mar 2013 | Category: Wife Lovers |
Avg Score: 5 |
Words: 7,050 |
Tags: wife masturbation cheating stranger hotel business flirting
| 18 Comments
husband watches wife fuck two men
I have written some true stories about our adventures, this is an account of a recent event. Jaq had gone to the pub a lunchtime with a work collegue, the two ladies had attracted the attention of two guys and were flirting with them. When it was time to go back to work Jaq slipped her phone number to the guys. Later on in the day Jaq got a text message from one of the guys asking to meet...Read On
Added: 02 Jul 2015 | Category: Wife Lovers |
Avg Score: 4.5 |
Words: 932 |
Tags: cock cage wife lovers
| 23 Comments
We make our wife watching fantasy come true
I am one of the luckiest men in the world. Let me tell you about my wife Joan. She is the smartest and sexiest woman I have ever known. She has a glorious body. She has very firm medium sized breasts that never need a bra, slim waist and a solid ass. She is also a natural exhibitionist. She loves to wear scandalous clothes that show off her body and excite men. Some of her favorite...Read On
Added: 16 Apr 2015 | Category: Wife Lovers |
Avg Score: 4.39 |
Words: 1,173 |
Tags: bi-sexual exhibitionism wild jamaican sex interracial
| 2 Comments
A couple explores swinging.
This Story is based upon several couples experiences that I melded to together into one story, I hope you like it. Let me start by introducing ourselves, I'm Karri and my husband is Terry. I know; Karri and Terry. We were made for each other. Terry is 28, 6' 200 lbs., his head is shaved after his hair started to recede but he is pretty cute that way. He keeps fit, as we both like the...Read On
Added: 21 Nov 2015 | Category: Wife Lovers |
Avg Score: 5 |
Words: 3,596 |
Tags: swinger fuck suck
| 26 Comments
Two adventures in one day, lead to finding one big secret.
Phone rang early on Saturday morning; I was still asleep, it was my weekend off work and wanted a lay in. I wasn't going to answer it but when I looked at who was calling; it was my brother's home number. I answered the phone, "Hello," in a groggy morning voice. It was my brother's wife Tracey. Tracey was twenty-two and an athlete, a four hundred meter runner. So having said that, you...Read On
Added: 20 Nov 2015 | Category: Wife Lovers |
Avg Score: 5 |
Words: 6,572 |
Tags: wife lover cheating big cock cunnilingus squirting athletic creampie
| 7 Comments
My cock sheathed in her pussy, Joe's cock naked in her mouth...
"So one day, I and my wife were busy fucking …" It wasn't strictly true, we weren't quite actually fucking, but we were teasing each other, and I jokingly asked her if she ever fantasized about having two cocks to play with at the same time. She grinned and said, "Well, duh! Who doesn't?" Well, I don't, for one, but the thought of my sweet Jennifer sinking her pussy onto a new cock...Read On
Added: 29 Nov 2011 | Category: Wife Lovers |
Avg Score: 4.85 |
Words: 1,724 |
Tags: sharing wife interracial creampie exhibitionist surprise
| 6 Comments
Wife helps neighbor while his wife is out of town
My neighbor, David, is happily married to his wife of 13 years, Sandra. They hail from the UK and have lived in the US for about the past ten years. They have three daughters which they adore. Every few years, they return to the UK to visit family. This year, Sandra and the daughters returned to the UK without David. Sandra and David planned to remodel their kitchen and it would be...Read On
Added: 31 Oct 2015 | Category: Wife Lovers |
Avg Score: 4.87 |
Words: 3,271 |
Tags: wife lovers cuckold lesbian neighbor voyeur
| 9 Comments
Grace never knew what she had been missing
Grace was agitated. She looked at the clock again 6:20 pm. Jason, her husband was due home at 6:00 pm. Jason had been gone for almost three weeks, working somewhere in Chicago. He was a junior architect in a private firm that specialized in all-glass buildings. He had called her yesterday telling her that he would be home Friday for at least the weekend. His flight from Chicago plus...Read On
Added: 24 Mar 2013 | Category: Wife Lovers |
Avg Score: 4.73 |
Words: 3,275 |
Tags: cuckold anal interracial blow job cheating wife lust
| 10 Comments
I recently started a new job and with it came a warehouse of young men and a few areas to hideaway for some masturbation if it was needed. As I got my tour on the first day I noticed not only the guys, but areas that I could possibly hide away for a few private moments but like most places of work there was always the chance of being seen. After the first week I noticed a...Read On
Added: 05 Apr 2009 | Category: Wife Lovers |
Avg Score: 4.18 |
Words: 879 |
Tags: wife lover mature straight
| 4 Comments
Wife meets a new gym partner
It's the middle of the semester and my wife and I recently switched gyms. Prior to the move, the two of us going together was fairly sporadic but with the new gym we had trustworthy child care and more convenient proximity to work, allowing us to meet up during our lunch break to lift. We are both early thirties and the average age of the members in the gym was early twenties. Even so,...Read On
Added: 28 May 2016 | Category: Wife Lovers |
Avg Score: 4.79 |
Words: 2,463 |
Tags: gym hotwife
| 7 Comments
My ass now loved it
Fantasy turned into reality "Hey cuckold, did you enjoy fucking Ann last night, would you like to fuck her again?" my wife shouted across the room. "Oh yes, it was fucking great and please can I fuck her again," I pleaded. "If you are a good boy, I will arrange another meet but I have another surprise for you this Wednesday," she said. This had become our sex party night and...Read On
Added: 19 Mar 2012 | Category: Wife Lovers |
Avg Score: 4.8 |
Words: 1,324 |
Tags: cuckold bi wifes lovers
| 1 Comment
Tied up wife enjoying a stranger!
It was a freezing cold night in January as Mike came in from the back garden. He grabbed two glasses, a bottle of wine and started to pour. "I'm off up for a shower now," said his horny little wife, Chloe. "Okay,"replied Mike. "Just remember, the children are at your parents for the rest of the night." "What's that supposed to mean?" asked Chloe, knowingly. "It means, when you come...Read On
Added: 20 Feb 2016 | Category: Wife Lovers |
Avg Score: 4.48 |
Words: 1,196 |
Tags: wife stranger tied table
| 6 Comments
It was Mike and Chloe's anniversary weekend, so Mike decided to take Chloe away overnight. The trip would start with a bit of shopping followed by plenty of eating and drinking. They said goodbye to the children and boarded the northbound train. Once they got going Mike opened a bottle of bubbly. "What are you up to?" quizzed Chloe. "I thought this trip was about retail therapy, not a...Read On
Added: 11 Mar 2016 | Category: Wife Lovers |
Avg Score: 4.94 |
Words: 2,242 |
Tags: wife flashing threesome
| 3 Comments
I breast feed in front of m husband's best friend
Setting the context : First, a little about me. I am twenty three, and married to my high school sweetheart. I am five foot, nine inches tall, with a slender athletic frame. My hair is blond and I frequently wear it in a ponytail, giving me a youthful, innocent look, even when naked. Normally, my breasts are small, but firm and perky with large prominent nipples. However, at the time...Read On
Added: 15 Apr 2016 | Category: Wife Lovers |
Avg Score: 4.98 |
Words: 10,024 |
Tags: breast feeding wife sharing orgasm
| 63 Comments
It was the 7th of August and I was with my husband. We had been invited to a barbeque by one of my husband's workmates. I had never met Mathew or his wife. But my husband said they were both friendly and nice people. Well, when we got into the car at this point I had no idea of how the day would end. Should also say that I was not in a mood to go somewhere on this weekend, because I...Read On
Added: 19 Nov 2011 | Category: Wife Lovers |
Avg Score: 4.55 |
Words: 1,375 |
Tags: swinging wifey
| 9 Comments


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BACtrack Keychain Breathalyzer Portable Keyring Breath Alcohol Tester, Black
- Advanced MicroCheck Sensor Technology - BACtrack's proprietary sensor technology delivers reliable, accurate BAC results you can trust.
- Ultra-Compact Design - Fits inside a purse or pocket and can be easily used as a keychain
- Effective Breath Capture - The innovative fold-out mouthpiece ensures only your breath goes into the breathalyzer, while ambient air, fumes, and other airborne gases are kept out.
- One Touch Operation - Simply power on your BACtrack Keychain and BAC results are displayed on a bright, easy-to-read LCD display in seconds
- Manufactured by BACtrack - The #1 most-trusted brand of breathalyzers, with over 14 years of experience providing the most accurate, convenient and affordable breath alcohol testing devices on the market
Frequently Bought Together
Customers Who Bought This Item Also Bought
Product Description
Product Details
Color: Black
From the Manufacturer
Available in: Purple, Green, Blue & Black
The Leader in Breathalyzers
BACtrack, one of the most-trusted brand of breathalyzers, with over 14 years of experience in the industry, provides the most accurate, convenient and affordable breath alcohol testing devices on the market.
With its personal, professional and smartphone breathalyzers being sold in more than 20,000 retail stores in North America and in over 25 countries worldwide, BACtrack truly is the leader in breathalyzers.
BACtrack Keychain Breathalyzer
Perfect for BAC testing on the go
Quickly and easily estimate your blood alcohol content with the small and affordable BACtrack Keychain Breathalyzer. The folding mouthpiece pulls out during testing and slides back down during storage – blow for accurate BAC results in just 5-seconds. Best of all, you can't forget your Keychain Breathalyzer as it travels with you wherever you carry your keys.
- Ultra-portable, take anywhere design
- Easy to read, two-digit LED display
- Accurate, reliable results from 0.00-0.40% BAC
- Operates with one AAA battery, included
- FDA 510(k) cleared for personal use
- Full one-year warranty
BACtrack Keychain Breathalyzer Features
The Smallest Breathalyzer Ever Created
Measuring just 1.5 inch x 2.5 inch x 0.5 inch and weighing a mere 2 ounce, the BACtrack Keychain Breathalyzer is the smallest, lightest breathalyzer in the world. Its ultra-compact size makes it easy to carry in a purse or pocket and can even be used as a keychain.
Reliable, Accurate BAC Results
Powered by Advanced MicroCheck Sensor technology, the BACtrack Keychain Breathalyzer delivers reliable, accurate BAC results you can trust. You can expect up to 75 tests per single AAA battery.
Share With Friends and Family
With its innovative folding mouthpiece design and three included reusable mouthpieces, the BACtrack Keychain Breathalyzer is perfect for testing yourself as well as your friends and family. Gain peace of mind and keep your loved ones safe with BACtrack.
BACtrack best rated Breathalyzer Brand
In an industry-wide comparison of breathalyzers by Car & Driver Magazine, BACtrack was rated one of the best overall breathalyzer brand, being given high marks for accuracy, portability and price.
'Best of What's New in Health' Award
Popular Science awarded BACtrack its 'Best of What's New in Health' Award, praising BACtrack's breakthrough technology that allows people to know when to stop drinking and to stay off the roads.
Reviews From Around the Web
BACtrack breathalyzers have received glowing praise from several notable industry leaders, including:
- Entrepreneur
- Time
- Wired
- Yahoo Tech
- Business Insider
What the Press is Saying
'Incredibly precise and easy to use.' - Newsday
'The best way to monitor your blood alcohol level before the authorities do it for you.' - Examiner
'Helps people understand alcohol's effect on their bodies.' - Fast Company
'A handy tool to have around.' - NPR
'One of the smallest breathalyzers available.' - Popular Science
|BACtrack Mobile Smartphone Breathalyzer
|BACtrack Vio Smartphone Keychain Breathalyzer
|BACtrack S80 Professional Breathalyzer
|BACtrack Trace Professional Breathalyzer
|BACtrack S35 Personal Breathalyzer
|BACtrack Keychain Breathalyzer
|Ideal Use
|Personal & Professional
|Personal & Professional
|PersonalPersonal & Professional
|Sensor Type
|Fuel Cell
|Fuel Cell
|Fuel Cell
|Fuel Cell
|Accuracy Rating
|10 - Most Accurate
|7 - Very Accurate
|10 - Most Accurate
|9 - Extremely Accurate
|7 - Very Accurate
|6 - Accurate
|LCD Screen
|Bluetooth Connectivity
|ZeroLine - Estimates time to 0.000% BAC
|Battery Type
|Mini-USB Rechargeable Lithium Ion
|1 x AAA battery
|2 x AA batteries
|2 x AAA batteries
|2 x AAA batteries
|1 x AAA battery
|One Year Warranty
Important Information
Actual product packaging and materials may contain more and different information than what is shown on our website. We recommend that you do not rely solely on the information presented and that you always read labels, warnings, and directions before using or consuming a product. Please see our full disclaimer below.
Customer Reviews
Top Customer Reviews
When I first got this little doodad, I put the battery in and went hog playing around with it, drinking with some buddies.
The next day, it started acting really funny. It would turn on and start counting down, and then shut off almost immediately. If I left it alone for several hours, I could do a test, but then it was unable to do another test. Sometimes I could do a test and it gave me the 'Lo' message. I thought the battery was dead. I checked the battery with a volt meter, and the battery didn't look dead at all. I thought I got a lemon... and then I got a new battery. Now it's fine.
To those who have seen the "it was working great the first night, and then it quit on me" reviews, this is what's going on:
This device seems power hungry, and I have a feeling that the AAA battery can't immediately produce the power it needs, so it needs to harvest the energy slowly while not in use so that it can do a test. I didn't like that this behavior isn't explicitly explained in the manual. The manual does say to wait a few minutes between tests. If you don't follow this, it will actually kill the battery. If you played with it non-stop all night and it won't work anymore on a seemingly good battery, replace the battery and use it properly. It will function perfectly. This is something that should be clearly addressed in the manual, and I think would eliminate the negative reviews that I have seen.
Realize, this device was designed for a responsible drinker who wants to stay safe. That person will use it perhaps 4 times at most throughout an evening.Read more ›
Will update this after I compare it to the BACtrack S80 fuel cell model that I recently ordered arrives.
Update: Drank two beers 24 oz. total. Here are the results as my body eliminated the alcohol. Three digit numbers on the left are the pricy fuel cell model results.
6:56pm .029 vs. .06 BAC
7:12pm .022 vs. .04
7:21pm .020 vs. .03
7:38pm .016 vs. .03
8:36pm .000 vs. .00
The two digit numbers on the right are this inexpensive keychain model. The three digit numbers on the left are the BACtrack S80 $136. model. My stomach was completely empty when I did the above comparison. My old alcohol sensor unit was more accurate than this one. This is little more than a keychain trinket/party toy as far as I am concerned.
Blew into a fuel cell model years back when a cop thought I was drunk. He was puzzled for I was well below 0.08 but had alocohol on my breath... Am glad I have an accurate breathlizer now. Cheap insurance considering the potential ramifications of being over 0.08% BAC. Figure on the high probability of a blood test for any and all drugs if you look/smell/act impaired. The courts have upheld the use of blood tests at the discretion of the cops. In the event of a fatal accident, your blood will be tested.
Went partying yesterday afternoon. Got two back to back readings with my S80 model of 0.Read more ›
When used correctly, it registers consistently exactly 0.01 below my other unit. I don't know which is "correct", but with mathematical computations based on my body weight vs. number of fluid ounces of alchohol, both register either exactly or very close to what they are supposed to. When I say "correctly", I mean not within 15 minutes of having a drink, or no repeated blows too close together (as the directions clearly state and clearly what multiple reviewers chose to ignore.)
I had one isolated incident where it read 0.00 a couple of consecutive times, but setting it down for 10 minutes and trying again seemed to correct it's minor brain lapse.
The real negative here is that the battery is seemingly only good for a few days (with our use, maybe 10-12 blows.) I would love for other users (or the manufacturer) to chime in and let me know if this is a defective unit, or truly a design deficiency, because this would be a 5-star product without this issue. Regrettably with it, it's only 3.
After publishing this review, I mailed bactrack customer service. They responded within about 4 business hours of my request with this very nice reply - upping my rating to 4 stars, and will change to 5 if the new one gets the proper battery life:
Thank you for contacting us with your Customer Support issue. I'm sorry to hear that you are having a little trouble with your breathalyzer.
According to manufacturer specifications and general customer experience, the KC10 typically has a battery life of about 50-75 uses.Read more ›
Customer Images
Most Recent Customer Reviews
I bought two of these so that we would never risk driving while under the influence, since a DWAI is .05 in Colorado (as little as one drink). Read morePublished 1 day ago by Liz Atwood
I originally had on of the original key chain BAC trackers that you could get at Costco only and it worked great. Read morePublished 2 days ago by Adam
Fun little gadget to see just how drunk you are. I would not use it as a gauge to see if you're okay to drive. Be smart, don't drink and drive. Read morePublished 2 days ago by Amazon Customer
my friends and i love having contests with this...who can get the highest! lol works great...just keep it in your car to test yourselfPublished 9 days ago by Amazon Customer
Secret Santa present. I haven't heard anything bad, so I'm sure it's fine. It's probably not too accurate, but it does keep the party going as everyone tries to get the high... Read morePublished 9 days ago by Steven
Works better and easier than I even imagined it might. Nice to keep in the armrest of my car just in case. Read morePublished 10 days ago by The Puzzle Maker
It works well and gives you a general idea of your BAC but it is not as accurate as similar products that are slightly more expensive. Read morePublished 11 days ago by Erica
Horrible!!!!! Would give it 0 stars if I could.
Not ever accurate. No matter what it reads .00. DO NO BUY THIS PRODUCT!!!!!!


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Here's a view of what would be the back of the wedding invitation. I was thinking these would also look pretty nice blind-embossed. The orange color was just...
February 14, 2012
Well, I said I'd post a few shots of my finished samples for the Bridal Expo (today! aaaah!). Here's my favorite. The paper is handmade from Etsy and the arc...
February 12, 2012
Letterpress arcade-inspired invitations.
Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2016 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


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12 articles
53,948 page views for Sista Irie articles and news.
Sista Irie has been a radio producer and program host since 1989 at 88.7 FM KAZI in Austin, Texas. KAZI focuses on the African American community and features the roots of black music through the education as well as entertainment. Sista Irie hosts "The Conscious Party" every other Sunday from 2-5 CST in an effort to promote the serious societal messages of reggae music. She is also a photojournalist, shooting many of reggae's best known stageshows within the United States and Jamaica. Sista Irie is married to Rastafarian elder, Brother Ricky, and enjoys a home both in Westmoreland, Jamaica and Austin, Texas.
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In 2016, the Organisation of the Swiss Abroad is celebrating its centenary and 100 years of working on behalf of the Swiss Abroad. The anniversary year takes the title of 'Switzerland – part of the world'.
The programme of the 94th Congress of the Swiss Abroad which will take place from 5-7 August 2016 in Bern is now available online (in German and French).
The Swiss Post Office is joining in the OSA's centenary celebrations by issuing a special stamp. After a competition organised by the Swiss Post Office, it is Tom Künzli who was given the tricky task of representing the diversity of the Swiss Abroad on a few square centimetres.
Watch the live stream of the centennial festivities, the meeting of the Council of the Swiss Abroad and the congress conference of the 94th Congress of the Swiss Abroad on our website.
Over a two-week period, 100 young Swiss Abroad – divided into four groups – will set off from the four corners of our nation to discover Switzerland. They will report on their adventures on the Swissinfo website and in the SSR-SRG media outlets.
Who is the OSA?
The Organisation of the Swiss Abroad (OSA) represents Swiss expatriates' interests in Switzerland. It informs Swiss living abroad about what is happening in Switzerland and provides them with a wide selection of services.


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Sign In
In a thin provisioning environment, storage usage is shown in terms of consumed and subscribed capacities:
By default, reclamation reports use subscribed capacity when they calculate thin LUN capacity. If you want reclamation reports to use consumed capacity when they calculate thin LUN capacity, select in the upper-right corner of the report. The Use Consumed Size option is available in the following reports:
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See Host Storage Assessment report: assess reclaimable storage across datacenter hosts.
Allocated LUNs Reclamation report
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Terms of use for this information are found in Legal Notices.


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Genre: Talk
Premise: Over the course of 25 years, many guests -- famous and not -- graced the stage of ``The Oprah Winfrey Show'' to chat with the legendary talk-show host. But viewers don't always find out what happens to the guests once they leave the stage (what ever happened to that Tom Cruise guy after he jumped on her couch) until now. ``Oprah: Where Are They Now?'' revisits some of Winfrey's most-famous -- or in some cases most-infamous -- guests to see what they're doing today. Returning guests include musical group Hanson, beating victim Rodney King (in his final interview before his death), and shooting victim Mary Jo Buttafuoco.
Hulk Hogan says that his life fell apart after "Hogan Knows Best" was canceled in 2007, and that ...
"When our kids grow up, they may see their father's latest interview and wonder why he made untru...
A Different World Cast Reunion; Soap Star Finola Hughes, Kiss' Paul Stanley
S04, E10 (First Aired: Oct. 26, 2014)
The cast of ``A Different World'' reunites; updates from ``General Hospital'' actress Finola Hughes and Kiss' Paul Stanley.
Bo Derek, Robin Leach, Eric Roberts & the McCaughey Septuplets Turn 17
S04, E15 (First Aired: Nov. 30, 2014)
Actress Bo Derek; actor Eric Roberts; TV personality Robin Leach; the McCaughey septuplets turn seventeen.


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David S. Dockery is president of Union University in Jackson, Tennessee. He has also served as chairman of the board of the Council for Christian Colleges and Universities. Dockery has authored or edited thirty books including Renewing Minds, Shaping a Christian Worldview, and Christian Leadership Essentials. In addition, he serves as a consulting editor for Christianity Today magazine.
George H. Guthrie, author of Read the Bible for Life, is the Benjamin W. Perry Professor of Bible at Union University in Jackson, Tennessee. He helped establish and is now a Senior Fellow at Union's Ryan Center for Biblical Studies, which is committed to promoting sound Bible reading, study, and interpretation at the grassroots level of the church. Guthrie has also participated in developing or revising several popular Bible translations including the HCSB, English Standard Version, and New Living Translation.


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Ezekiel Elliott signed a huge deal with the Dallas Cowboys Wednesday.
Ohio State wants walkers only on Oval
COLUMBUS, Ohio - Responding to a series of accidents on or near campus last fall, Ohio State University officials said they only want walkers on its iconic Oval and will tell bicyclists and skateboarders to stay clear.
The university is starting an information campaign on April 17 to make sure students understand traffic-safety rules and keep the Oval's sidewalks pedestrian friendly. Starting in the fall, police will write tickets to violators, an OSU spokeswoman said.
Signs posted around the Oval's perimeter will declare it a "walk zone."
"This is about starting a culture change on campus," Ohio State spokeswoman Lindsay Komlanc told The Columbus Dispatch (http://bit.ly/11OJtSS ) for a story Thursday.
The walk zone was among recommendations by a university task force created after a series of pedestrian, bicycle and motor-vehicle crashes during the fall semester last year.
The task force also raised concerns about bicyclists who do not know the rules of the road, and jaywalking and distracted pedestrians who might not be aware of the traffic around them. The university held an event recently focused on jaywalking and teaching students and faculty and staff members about the law.
Some students see the new policy as drastic.
Allison Chapman, 20, said she thinks fining people would be "a little excessive."
"Bicyclists sometimes get in the way," she said. "It's part of the process of getting to class. This is a main passageway."
Emily Lebsock, 19, also questioned the policy.
"I don't think it's fair," she said. "It's how people get to class. I've never been run over by a bike or skateboard of anything."
Campus accidents in the fall left three students seriously injured. An 18-year-old student was hit by a car while walking, another 18-year-old student riding a bicycle was hit by a dump truck, and a 21-year-old woman was hit when she stepped in front of a bicyclist.
Related Stories
Ezekiel Elliott fans were happy to learn during the draft Wednesday that the running back didn't leave his famed fashion trend behind when he left Ohio State after last season.
Ezekiel Elliott already has a lofty spot in Dallas Cowboys lore. Now the former Ohio State running back has to try to live up to it.
Ohio State running back Ezekiel Elliott will be surrounded by several familiar faces for the first round of the NFL draft.
UPDATE: The 'shelter in place' alert was lifted after 2 p.m., according to OSU EMFP.
An attorney for the family of Will Smith says he did not brandish a gun or have it on him before he was shot to death.
A former marching band director fired from Ohio State University after an investigation into what the school said was a sexualized culture within the band has been hired by a small university.
A woman is in critical condition after she fell out the window of a sorority house at Ohio State University overnight.
Basketball's king has revealed who he will be rooting for during the National Invitation Tournament in Ohio.
Tickets for the National Invitation Tournament, in which Ohio State takes on the University of Akron, go on sale to the public Monday.


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Pueblo Police confirm that a fatal accident that occurred on E. 4th St. near N. Hudson Ave. around 4:20 p.m. Tuesday. The name of the victim has not yet been released.
Investigators say the man crashed his scooter into the back of a 1995 Honda Passport being driven by a 73-year old man.
The SUV was stopped for traffic mid block on 4th street. Just as it began to move forward, it was hit from behind by a scooter driven by a 52-year old man.
The scooter driver had been unable to stop in time and the scooter slid on its side for about 30 feet before it hit the Honda.
Police say the scooter rider was not wearing a helmet at the time of the accident.
The scooter rider was taken to Parkview Hospital by ambulance and died a short time later.
The driver of the Honda Passport was not injured.
Alcohol and speed do not seem to be a factor in this accident.
Stay with 11 News and KKTV.com for more information as it becomes available.


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Strona główna
Informacje Konsularne
About Yemen:
Climate and weather
Geographical Divisions
Historical Background
Yemen Parliament
Central Bank of Yemen
Ministry of Planning
General Investment Authority
Ministry of Planning
Tax Authority
Customs Authority
Ministry of Oil
and Mineral Resources
Ministry of Human Rights
The Republic of Yemen lies in the South of Arabia (The Arabian Peninsula), south-west of Asia, between latitudes 12 and 20 degrees to the north of the equator, and between longitudes 41and 54 degrees to the south of Greenwich, bordered on the north by Saudi Arabia, on the south by the Arab Sea and the Gulf of Aden, on the east by the Sultanate of Oman and on the west by the Red Sea.
The administrative units of the Republic of Yemen consist of 19 Governates and the capital secretariat, the city of Sana'a. Each Governate is composed of a number of' districts and centers, the Governates are as follows: Sana'a, Aden, Taiz, Hudaidah, Hadhramout, Sa'da, Al .Jouf. Marib, 'Shabwa, Abyan, Dhamar, Ibb, Lahj, Mahweet, Al-Mahara, Al-Baydha, Hajja, Amran, Al-Dhalia.
Climate and weather
Since Yemen is endowed with a varied topography, the climate, in general, also varies, although there are no major seasonal differences. We can say there are two long seasons: summer and winter During summer, the temperature and humidity are high in the coastal areas, while the climate is moderate during winter. From time to time the rainfall, due to the monsoon, moderates the temperature over the coastal areas in summer. In the mountain areas, the weather is moderate in summer and cold in winter during the night and in the early morning, but the bright sun rays raise the temperature during the day making the weather very moderate in these parts of the country.
Geographical Divisions
In view of its topographical structure, the Republic of Yemen is divided into five regions: Mountain, Highland, Coastal,the Empty Quarter, and the Yemeni Islands.
1. Mountain region with heights ranging between 1000-3760 m high
The western mountain range represent a large area of the country. It is the range called Al-Surat mountains stretching longitudinally from the north to the south and transversally from the west to the east which resembles the letter "L". These heights go down in different directions. They descend sharply westwards to the Red Sea, southwards to the Gulf of Aden, and eastwards and northwards to the interior desert areas. In the mountain heights, stretching from the north to the south, there are a number of basins (beds), of considerable agricultural importance with large populations, such as Sana'a basin, Yarim basin, Jahran flatland, Sa'da flatland, Alboun flatland, Aqwat Hadhramout bed and Si'aith bed in Mahara. The most prominent mountains in these heights are the mountains of Attaq, Bayhan, Mukairas, Al-Dhalie', Yafie', Sabra, Hudhour Alsheikh, Miswar and Prophet Shu'aib which at 3766m above sea level is the highest mountain in Arabia.
2. The Highland region
This area lies to the east and north of the mountainous heights going in parallel to the heights towards the Empty Quarter. The maximum height of this area is 1000m gradually descending. Such area is represented by the highlands in Sana'a, Al Jouf, Shabwa, Hadhramout and Mahara. The out- skirts of this area in the north overlap with the Empty Quarter.
3. The Coastal Area
This area includes the coastal plains overlooking the Red Sea, Gulf of Aden and Arabian Sea. They are connected to each other forming a coastal strip that extends from the Oman border south- westward to Bab Al-Mandab. This strip then changes its direction northward to the borders of Saudi Arabia, thus making it more than 2400km. long. The width of the plains range from 30 to 60 km.
4. The Empty Quarter
This is a Yemeni desert area located to the north of Hadhramout highlands, and to the south of the western heights. This area consists of desert plains covered with gravel, sand and sandy dunes in Volving in some parts desert plants and vast oases, that were formed due to the collection of the seasonal water. It is inhabited by the wandering nomads because of its pastoral land.
5. The Yemeni Islands
This part of the country consists of islands scattered along the coasts of Yemen. There are 120 islands, most of which are located in the Red Sea. The biggest islands are Kamaran, Greater Hunaish, Minor Hunaish, the rest of Archipelago, Zaqar, Zubair, AL-Tayr and the strategic island of Meon at the Bab AI-Mandeb. In addition there are islands located in the Gulf of Aden and thc Arabian Sea, the largest of which is Soqatra.
Historical Background
The diversity of the natural environment: plains, mountains and deserts may be common in many countries, but it is unique in Yemen. Yemen's highlands are an extension of a chain of the Trans-Arabian Peninsula's high mountains which cut across, in parallel, from the Red Sea in the west to the Arabian desert, The Empty Quarter, in the east, the shape of which resembles the letter "L". Because of the height of this mountain chain, which is the highest in the Arabian Peninsula, rain clouds, carried by the seasonal winds from the Indian Ocean during the spring and summer, causes rainfall, which differs greatly from 1he rest of the area.
The rains are not normally heavy, but when it pours, torrents flow across a number of valleys. Some goes east to the desert and some west to the Red Sea, or south to the waters of the Arabian Sea. Under these circumstances and climate, people of the Stone Age lived here tens of thousands of' years ago experiencing the various stages of that period. There are many relics dating hack to this era are now in exhibit at the National Museum in Sana'a. The facade of caves show distinct traces of the Hunter Gatherers Society. While on top of the eastern mountain chain, 2000*2300m above sea level, more sophisticated societies lived during the Bronze Age.
Unsuccessful attempt at dam construction can be traced back to the bronze age. The people, after this failure, emigrated down-stream to the valleys in the East of Yemen, and it is here where civilization in this part of the world began to flourish. Yemeni civilization didn't flourish on the hanks of rivers, as in many other ancient civilizations, but in the valleys. The Sheba dynasty flourished in the valleys of Dhanah and Ma'een in the Jouf Valley to the north of Sheba. Similarly, other dynasties such as Qataban in the Baihan Valley, Hadharmout in the valley of Armah and Osan in the valley of Markhah.
is the oldest of the Yemeni civilizations. Historians consider the start of the Sabean Dynasty as the beginning of historical accounts for Yemen's ancient states. This historical period began in 715BC, the year when Yathea'a Amirbayn, one of the oldest Macarabi, King of Sheba, paid protection money to Serjoun, the King of Assyria, as told by the Assyrian annals.
The first line of Sabean Macarabis and kings existed around the same time as the kings of Ma'een. The only time Ma'een was defeated was by Sheba, its neighbor. The sovereignty of Sheba also extended to cover the ancient states of Osan, Qataban and Hadramout. Qataban and Hadramout had sided with Sheba against the State of Osan.
Regardless of which Yemeni dynasty was the oldest, strongest or reigned longest" the most recent archaeological researches state that the Iron Age extended from 1200BC until 332BC. Thus, the beginnings of the flourishing history of Southern Arabia civilizations was 1st century BC. The people lived by means of agriculture and it was around this time that dams and canals were constructed to irrigate the land to provide food and camels domesticated to help with the work load. The development of political systems was similar in each dynasty. Their location, between 1ndia and Africa, on one side, and between Egypt and Syria on the other, generated a good income from the taxes paid for the protection of the camel caravans transporting incense from Hadhramout and Dhofar (Oman) in the East. These caravans were also used to carry other goods, whatever would fetch a good price and was light enough for the camels to carry, which arrived into Yemen at the port of Qana on thc Arabian Sea. From there they would be transported through the cities and stations of Hadhramout, Qataban, Sabean and the Ma'een Dynasties on to the port of Gaza on the Mediterranean coast. Cities flourished and the architectural style developed. The temples of the Astrological Triangle' were built along with the dams and canals which surrounded the capital cities of Marib, Ma'een, Tamna'a and Shabwa.
The capitals of the civilizations of southern Arabia had strong contact with ancient Eastern civilizations. Before the birth of Christ, a campaign by a Roman Commander failed in an attempt to take control of' the incense road. However, Hippalos, a Greek sailor, had discovered the closely guarded secret of the ancient Yemenis: the monsoon winds, which gust eastward to India during the summer and to the west towards Africa during the winter and made transportation by sea so very difficult. The discovery by the Greeks on how to use the monsoon winds to their advantage, was followed by a ban, introduced by the Christian Church, on the use of incense which resulted in the incense road losing its importance. The Great Dam also collapsed and the Sheba Dynasty dispersed to the hills. This was evident by the dams, cities, palaces and temples which sprang up in Sama'i, Sana'a and Dhafar, and also on the trade road across the plateau by way of the Assa'd' path.
The sand dunes, however, preserved the outer crust of the ancient Yemeni civilization in Eastern Yemen, in a way similar to the process of preserving flowers pressed within the pages of a book, and as a result, archeologists have been able to trace the history of ancient Sheba.
The Himyarite Dynasty followed with its rulers, the Kings of Sheba, Dhu Raidan, Hadramout, Yamant and their tribes on the high plateau and coastal region, until the Abyssinian (Ethiopians), occupied Yemen in the year 525AD. The Abyssinians ruled for fifty years until 575AD. That year was known as the Year of the Elephant', because Abraha used them in his failed campaign to occupy the Qa'ba, Islam's most holiest of shrines. Saif Ibn Dhi Yazan expelled the Abyssinians from Yemen with the help of the Persian Empire, but only to place Yemen under the direct influence of Persian Empire. This continued until the people of Yemen heard the Islamic call. They voluntarily adopted Islam as their new religion; and Yemeni battalions were at the vanguard of the Islamic armies. Yemenis played leading roles in the building of the Islamic state, which stretched from China in the east to Andalusia to the west.
Yemen came under the direct administration of the Islamic state during the rule of the Caliphs in Madina, continuing through the Ommiad Dynasty in Damascus and the Abbasid Dynasty in Baghdad. However, on the decline of Islamic state in Baghdad, several dynasties ruled Yemen starting from the 9th century AD. At various stages in the history of Yemen there was more than one dynasty in existence, fighting each other at times and peacefully co-existing at other times. Some of them had formally followed either the Caliphate of Baghdad or the Caliphate of Cairo. The direct rule of some of these states included the largest parts of Yemen. Among these states were:
Ziyadiya State
the capital of which was Zabid, 819 * 1018AD.
Ya'firiya State
in Shibam kawkaban, 861* 956AD.
Zaydi Imamate
State in Sa'da, 892*1962AD.
It co-existed with all other states for more than one thousand years. Its influence stretched from Sana'a to Najran, diminishing to include only the north-eastern regions. The Zaydi Imamate ruled most parts of greater Yemen at one period in history.
Sylihya State
in Jibla, 1047 - 1138AD.
Ayoubid State
in Taiz, 1174 - 1229AD.
Rasulide State
in Taiz, 1229 - 1454AD.
Tahirid State
in Mikrana Rada'a', 1446 - 1517AD.
Despite the fact that these states were independent from the central states, they were in touch with what was going on in the main cities. Several Yemeni cities flourished during the lslamic era on the roads of trade and pilgrimage across the plateau and coastal plains. They were influenced by the Islamic civilization which also prevailed at other Arab and Islamic cities. Students and scholars came to the centers from both inside and outside Yemen. The most famous cities of the period were Zabid, Sana'a, Sa'da, Tarim, Jibla and Dhamar. Yemen also fell under the influence of Islamic states such as the Mamelukes and the Ottomans in the wake of the Portuguese invasion of the coasts in the early 16th century AD.
The Turkish Ottomans ruled Yemen during two periods. The first began in 1535 and continued until 1638, while the second was from 1872 until the end of the 1st World War in l918. The British occupied the southern part of Yemen in 1839.
During the period of conflict over the borders between the Ottomans in the northern part of Yemen and the British in the south, border posts were installed between the South and North of Yemen for the first time in its history. After that, Yemen suffered from terrible isolation imposed by a clerical utilitarian rule in the North and a humiliating colonial hegemony in the South.
During this period, poverty, ignorance, injustice and a series of deadly diseases prevailed in the country. The circle of isolation was not broken until the Eternal Revolution of the 26th September against the Imamate rule and the Revolution of the 14th October 1962, and the South achieved independence from Britain in 1967.
From 1962 North Yemen became known as the Yemen Arab Republic, with its capital in 'Sana'a, while the South of Yemen became known as the People's Democratic Republic of Yemen with its capital in Aden. Twenty years after the British evacuation on 30th November l967, the constitution of the existing Republic of Yemen was signed on 30th November, 1987. This event crowned pro- longed efforts to unify the country. On 22nd May 1990, there was great news, Yemen became united and was named the Republic of Yemen. The historic city of Sana'a was named the capital of the unified Yemen, and a new era in Yemen's history began.
Created by
, 2009


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Developer- and Publisher-specific Articles: GOG
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GOG.com has added the surreal point-and-click adventure title Toonstruck to their library. The 1996 adventure title from Interplay follows cartoonist Drew Blanc as he attempts to escape a strange cartoon world populated by a wide variety of weird creatures—ah, the '90s. The game also features digitized actors, and a handful of notables are involved, including The Simpson's Dan Castellaneta, Christopher Lloyd, and Tim Curry, also star of Interplay's Frankenstein: Through the Eyes of the Monster.
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GOG.com has teamed up with publisher Devolver Digital to offer five of their titles and 10 of their films in a new sales promotion. From January 30 until February 3, individual games and movies can be purchased at 50% off or in an all-in-one bundle for $13.75, a discount of 90%. A full list of all the films and titles currently on offer, which includes the 2013 Shadow Warrior, Hotline Miami, and Mars, can be found below.
GOG.com adds Rogue Squadron 3D, Rebellion, and Empire at War
After adding six LucasArts' titles to the service on Tuesday, GOG.com has announced that three more games have gone live today and three more will join them on January 27. As for today, the three games that have been added include Star Wars: Rogue Squadron 3D, Star Wars Rebellion, and Star Wars Empire at War: Gold Pack. Both Rogue Squadron 3D and Rebellion are making their digital debut. Next week will see the addition of Dark Forces II, Republic Commando, and Starfighter.
GOG.com adds X-Wing vs. Tie Fighter, X-Wing Alliance, and more
Flight sim fans rejoice! When GOG.com added X-Wing and Tie Fighter to their library, the service noted that there were plans to add more of LucasArts' popular but neglected catalog to the service in the near future. It seems that those plans are now coming to fruition with the announcement that six Star Wars titles have been added today, several of which are making their long-awaited digital debuts: X-Wing vs. Tie Fighter, X-Wing Alliance, Galactic Battlegrounds, Battlefront II, Dark Forces, and Knights of the Old Republic II: The Sith Lords. If one of your old favorites wasn't mentioned, fear not, 12 more titles are scheduled to go live over the next ten days.
GOG.com begins Big Fall Sale with discounts, copy of Mount & Blade
GOG.com has kicked off their Big Fall Sale. As has become a trend in recent years, the service will be offering free games throughout the promotion, including Mount & Blade and The Witcher 2: Assassins of Kings. The original Mount & Blade is the first freebie, and the first bundle of the day features Blade: Warband and Mount & Blade: With Sword and Fire for 75% off. By the end of the sale, over 700 titles will have been discounted for up to 90% off through on-going sales, two daily bundles, and 40-minute flash deals.
GOG.com adds Tie Fighter, X-Wing, Monkey Island, and more
Several of us here have been waiting years for this news: GOG.com has added Star Wars: X-Wing Special Edition and Star Wars: Tie Fighter Special Edition to their library. Actually, the service added six games in total as part of a new deal with new license holders Disney Interactive, including Indiana Jones and the Fate of Atlantis, Sam & Max Hit the Road, The Secret of Monkey Island: Special Edition, and Star Wars: Knights of the Old Republic. All are fantastic, but the two space-based combat flight sims in particular have been sorely missed. And this isn't all. This batch represents only the first wave of a 20-plus-title deal.
GOG.com giving away Aliens vs. Predator, Linux library grows
For the next 48 hours, GOG.com is offering a free copy of Aliens versus Predator to users who sign up to beta test GOG Galaxy. GOG Galaxy is the service's upcoming multiplayer client, and the game is being provided as a way for gamers to try the system and for GOG to see how the infrastructure holds up under increased stress. Bearing in mind that this is Aliens versus Predator Classic 2000, one of the better titles to use the licenses, and that the game will remain in the user's library, this is a pretty nice bonus. A cross-compatibility update with Steam is set to go live "soon." GOG-ers (if we can make that a thing) can head here to sign up for a copy.
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Shadowgate now on GOG at 20% off, with ringtones and other extras
Zojoi's re-imagining of the classic point-and-click adventure title Shadowgate is now available on GOG.com. To promote the launch, GOG.com is offering a 20% discount on both Mac and Windows versions. The service is also including several digital bonuses: an exclusive comic-style hint book, five wallpapers, and 13 downloadable ringtones taken from the NES version. With those ringtones, it'd be like every call is a mystery waiting to be unraveled!
GOG.com redesigned, adds section for DRM-free movies
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Fill these Halloween Mini Cookie Tin Favors with Halloween candies and cookies!
Our Halloween Mini Cookie Tin Favors are ideal for Halloween candies and cookies. Give the Halloween Mini Cookie Tin Favors as a prize for your party's costume contest winner or take grandma and grandpa a treat when you go trick or treating. Just choose one of our fun Halloween designs and add your own spooktacular Halloween message. Each mini cookie tin measures 6 inches in diameter x 1 3/4 inches high and is made from tin making it reusable.
Please allow ample time for delivery. Our ship date for this product is noted in your shopping cart. Many items are shipped separately, so please allow extra time for these items to arrive. Shipping charges are based on the value of the merchandise and not the number of shipments. For additional shipping information, contact our Customer Service Department at (firstname.lastname@example.com or 1-800-227-2789).
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Release Date:
May 1, 2002
Date Published:
May 1, 2002
Just released from prison, Shadow encounters Mr. Wednesday, an enigmatic stranger who seems to know a lot about him, and when Mr. Wednesday offers him a job as his bodyguard, Shadow accepts and is plunged into a dark and perilous world.
In a strange and wonderful science fiction tour-de-force that takes place in a contemporary America infested with strange and mythical beings, a recently released convict named Shadow accepts, perhaps unwisely, an ill-defined job from a mysterious man named Wednesday. He soon finds himself involved with ghosts, knights, and alternate planes of dimension as he is recruited into a battle, involving forces of myth and modernity, that he hardly understands. Winner of the 2001 Bram Stoker Award for Best Novel as well as the 2002 Hugo and Nebula awards.
Genre: Fiction + Literature Genres
Sub-Genre: Science Fiction + Fantasy
- Genre: Fiction + Literature Genres
- Subgenre: Science Fiction + Fantasy
- Publisher: HarperTorch
- Pages: 592
- Edition: Reprint
- Language: English
- Release Date: May 1, 2002
- Author: Neil Gaiman
other Info.
- Online Item #: 11829505
- Store Item Number (DPCI): 248-25-7986
- Item can be gift wrapped.
- Made in the USA or Imported


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Contract Type: Permanent
Category Engineering & Architecture


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A Common Referance To The Cappuciono.
I Would Like The Pooche.
by Pooche Ftw January 13, 2009
Trending Now
Top Definition
A moustache belonging to a person from pakistan
i said to my brother you need to shave your pooche.
by sascha bismarck March 23, 2009
the sound a vagina makes when you pull out. also used for randomly saying something when nothing else to say.
dude, that sounded like a pooche. (awkward silence) POOCHE!!
by rmzman27 February 01, 2011


text:
A very good friend recently confessed to me that sometimes their subconscious sugar cravings are so powerful, they get out of bed in the middle of the night to eat sweets.
This is what sugar — dubbed 'sweet poison' by NY Daily News — is doing to us. We've become a generation of zombies that seek sugar at any cost, even if it means interrupted sleep, expanding waistlines and poor emotional health. The worst part is, we don't even realize it's happening.
Cookies and candy get a lot of criticism, but at least these sugary foods are honest about what they contain. The real danger lies in foods that hide their sugary nature behind fancy monikers and mile-long ingredient lists. These hidden sugars make it possible for the average American to consume 152 pounds of sugar each year, despite filling their grocery carts with savory staples and so-called health foods. Once eaten, sugar affects the brain almost exactly like another powdery white substance we know and fear – cocaine — triggering an intense addiction that compels us to seek more.
Read: 5 Health Hazards of Excess Sugar
The first step to getting off the sugar addiction roller coaster is exposing these hidden sugars. There are literally thousands of foods that conceal this sweet poison behind savory labels, but these 10 are the worst, and most surprising. (Note: this list refers to the conventional and store brands many of us buy. Different rules apply to organic, artisan and homemade varieties. Obviously.)
10 Foods That Have the Most Hidden Sugar
1. Condiments
Grilling a hamburger? Mixing up a batch of Grandma's potato salad? Prepare for a sugar bomb. Ketchup, barbecue sauce, mayonnaise, steak sauces, salad dressing and, yes, even mustard all contain sugar. Some styles of BBQ sauce contain 13 grams of sugar (approximately 3 teaspoons) for every two tablespoons of condiment!
2. Yogurt
Yup. This is one of those "I thought it was healthy!" foods that are probably sabotaging your weight loss plans. An 8 ounce serving of the average fruit variety non-fat yogurt contains a staggering 47 grams of sugar (11 teaspoons)!
3. Pasta Sauce
Pasta with marinara sauce is a delicious savory meal. Too bad the hidden sugars make your body treat it like dessert. Store bought pasta sauce contains around 12 grams (around 3 teaspoons) of sugar for every half cup.
4. Dried Fruit
Unlike pasta sauce, we expect dried fruit to be sweet. Those trying to keep a healthy diet will often opt for this instead of candy, but it makes no difference to your body. Just ⅓ of a cup can have 24 grams of sugar (8 teaspoons) and it's not all naturally occurring: Most add a healthy dose of the refined stuff (as if fruit needs to be sweeter?!).
5. Granola Bars
For some reason, we've been duped into assuming granola = healthy. With 12 grams (4 teaspoons) of sugar, and sometimes much more, hiding in most conventional brands, you might as well be eating a Payday.
6. Bread
Love toast with your coffee? How about a sandwich for lunch? That innocent looking slice of bread contains around 2 grams of sugar per slice (nearly a teaspoon). Even worse, if you opt for cheap bread, it's likely added in the form of high fructose corn syrup.
7. Boxed Cereal
You don't have to be a nutritionist to know that Lucky Charms and Frosted Flakes contain added sugar, but it's hiding in "healthy" cereals, too. Just look at the labels of Raisin Bran (20 grams, or 5 teaspoons), Kellogg's Smart Start (14 grams, or 3.5 teaspoons) and Kashi GoLean Crunch (13 grams, or 3-plus teaspoons).
8. Peanut Butter
It doesn't matter whether you prefer chunky or creamy, if you're eating any of the popular peanut butter brands, you're getting a big dose of sugar — to the tune of 3 grams (a teaspoon) in each 2 tablespoon serving. And just think, most of the time we slather it on bread (sugar) with jelly (sugar) and then feed it to our kids!
9. Deli Meat
Bread and mayo isn't the only thing making our sandwiches unhealthy. Most deli meats are infused with corn syrup and/or aspartame (an artificial sugar) for some strange reason.
10. Tomato Soup
Another staple that most of us have eaten since childhood. Too bad there are 10 grams (over 3 teaspoons) of sugar in the average 1/2 cup serving of canned tomato soup (usually high fructose corn syrup). Not to mention 400 mg of sodium. Blech.
A Sweetener by Any Other Name: The morals of this story are: 1) always read the label, and 2) avoid processed foods at all cost. However, even if you do these things sugar can still sneak in. That's because it's not always called sugar. Check out this helpful post to learn how to spot other names for sugar on food labels.
Image via Thinkstock


text:
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The human CCCTC-binding factor, CTCF, regulates transcription of the double-stranded DNA genomes of herpesviruses. The architectural complex cohesin and RNA Polymerase II also contribute to this organization. We profiled the occupancy of CTCF, cohesin, and RNA Polymerase on the episomal genome of the Epstein-Barr virus in a cell culture model of latent infection. CTCF colocalizes with cohesin but not RNA Polymerase. CTCF and cohesin bind specific sequences throughout the genome that are found not just proximal to the regulatory elements of latent genes, but also near lytic genes. In addition to tracking with known transcripts, RNA Polymerase appears at two unannotated positions, one of which lies within the latent origin of replication. The widespread occupancy profile of each protein reveals binding near or at a myriad of regulatory elements and suggests context-dependent functions.
The human CCCTC-binding factor, CTCF, regulates gene expression by organizing DNA in the three-dimensional space of the nucleus. CTCF binds tens of thousands of sites throughout the human genome (Barski et al., 2007; Kim et al., 2007) and is necessary for the assembly of chromatin loops that bring together distal DNA to regulate transcription (Hou et al., 2010; Majumder and Boss, 2010; Majumder et al., 2008; Mishiro et al., 2009). Globally, the human genome is organized by long-range interactions (Lieberman-Aiden et al., 2009) and binding sites are significantly enriched at these nodes (Botta et al., 2010). CTCF also organizes the double-stranded DNA genomes of herpesviruses. Binding sites have been identified on the Epstein-Barr virus (EBV) (Chau et al., 2006; Day et al., 2007; Tempera et al., 2010), herpes simplex virus 1 (Chen et al., 2007), and Kaposi's sarcoma-associated herpesvirus (KSHV) (Stedman et al., 2008). Functional studies with EBV have focused on binding sites near the major promoters of latency and suggested possible roles for CTCF in the repression, activation, and insulation of latent transcripts (Chau et al., 2006; Day et al., 2007; Tempera et al., 2010). CTCF occupancy upstream of the C promoter arguably correlates with repression of EBNA2 transcription (Chau et al., 2006; Salamon et al., 2009). Mutation of a CTCF-binding site upstream of the Q promoter resulted in loss of EBNA1 transcription accompanied by the spread of repressive histone marks and CpG methylation (Tempera et al., 2010). Based on a myriad of functions, CTCF has emerged as a central component of transcriptional regulation in human and viral genomes.
CTCF may form complexes with diverse binding partners that include transcription factors, histone modifiers, and a variety of chromatin regulators (Wallace and Felsenfeld, 2007; Zlatanova and Caiafa, 2009). The CTCF protein consists of eleven zinc fingers (Filippova et al., 1996) and two flanking unstructured termini (Martinez and Miranda, 2010); both types of protein segments are capable of recruiting cofactors directly by molecular recognition (Dunker et al., 2002; Gamsjaeger et al., 2007). Specifically, the architectural complex cohesin and RNA Polymerase II (RNAP II) may play functional roles in CTCF-dependent transcriptional control. CTCF is necessary for cohesin positioning and colocalization throughout the human, mouse, and KSHV genomes (Parelho et al., 2008; Stedman et al., 2008; Wendt et al., 2008). Depletion of cohesin perturbs gene expression by the disruption of DNA looping and long-range interactions between transcriptional control elements (Hadjur et al., 2009; Mishiro et al., 2009; Nativio et al., 2009). RNAP II is thought to interact with CTCF directly through protein-protein interactions, resulting in colocalization at a subset of CTCF-binding sites (Chernukhin et al., 2007). Stalling of RNAP II tends to occur at sites of CTCF and cohesin colocalization (Wada et al., 2009), and a large fraction of CTCF sites are found within actively transcribed genes (Barski et al., 2007). The diversity and genome-wide distribution of the myriad of possible CTCF assemblies, given significant functional implications, remains actively investigated.
We initially hoped to use profiling of protein occupancy to determine the composition of CTCF complexes by identifying which proteins bind the same DNA at high resolution. Colocalization would strongly suggest molecular assembly and cooperation, perhaps characterizing the structural and functional heterogeneity or uniformity of complexes genome-wide. Indeed, we were able to do so and determined that CTCF colocalizes with cohesin but not RNAP II. In the midst of identifying binding sites, however, we also uncovered protein occupancy in unexpected regions of the EBV genome. We will discuss the functional implications of CTCF and cohesin colocalization as well as RNAP II binding outside regions of known transcription.
CTCF binds specific sites throughout the latent EBV genome. We identified fifteen sites of CTCF occupancy at high resolution across the entire unique sequence of the EBV genome using chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) (Fig. 1, Table 1). An overrepresented sequence motif, similar to that found in the human genome (Kim et al., 2007), is detected in every region of occupancy (data not shown). During the life cycle of EBV, only a handful of regulatory elements control expression of a few latent genes, but many more genes are transcribed upon lytic activation (Kieff and Rickinson, 2007) We find CTCF-binding sites proximal to many latent regulatory elements. Occupancy is observed near the C, W, and Q promoters that drive messages encoding for the EBNA proteins. Enrichment is also detected close to start sites of the EBER-1 ncRNA and RPSM1 BART transcript, as well as the LMP-2A and LMP-1 messages. We also find, however, CTCF-binding sites proximal to many lytic genes. For example, occupancy is detected near the promoter of BZLF1, an immediate early transactivator critical for the switch to the productive cycle (Biggin et al., 1987). Both transcripts expressed during latency as well as genes activated during the lytic cycle can be found proximal to CTCF-binding sites.
Cohesin colocalizes with CTCF throughout the latent EBV genome. We measured the occupancy of the kleisin subunit RAD21 and identified six binding sites for cohesin dispersed throughout the EBV genome (Fig. 1, Table 1). All of these cohesin-binding sites overlap with CTCF-binding sites at high resolution; no CTCF-independent sites of cohesin occupancy are found. We did not detect RAD21 enrichment at all sites of CTCF occupancy, presumbly due to weaker efficiency of immunoprecipitation with the RAD21 antibody compared to the CTCF antibody. Similar to the distribution of CTCF-binding sites, the overlapping cohesin-binding sites are found proximal to regulatory elements of both latent and lytic genes.
RNAP II does not colocalize with CTCF, instead tracking with regions of known transcription as well as appearing highly enriched at unannotated positions. We measured RNAP II occupancy and identified four significant regions of binding (Fig. 1, Table 2). The particular antibody used in our experiments, 4H8, was raised against an epitope containing phosphorylated serine 5 and therefore detects occupancy both at promoters and throughout a transcribed gene (Brodsky et al., 2005; Stock et al., 2007). Binding is observed at ~ 6.5–6.9 and ~ 6.9–7.1 kbp, regions corresponding to the two EBER transcripts that span ~ 6.6–6.8 and ~ 7.0–7.1 kbp (Rosa et al., 1981). The observed peaks are distributed across the entire gene, a pattern consistent with actively elongating transcription. Although the signals did not rise above our significance threshold, we also detected some RNAP II enrichment near other known transcription units. Occupancy at ~ 138.3–138.5 kbp corresponds to the initiation site of the RPSM1 BART transcript, and the binding at ~ 169.1–169.3 kbp occurs near the promoters of the LMP genes (Fig. 1). In addition to sites of active transcription, we observed the strongest levels of enrichment at two novel positions (Fig. 1, Table 2). Recruitment occurs ~ 1 kbp upstream of the EBER-1 transcript at ~ 5.9–6.0 kbp. Occupancy is also strong at ~ 8.1–8.3 kbp, inside oriP, the proposed latent cycle origin of replication (Yates et al., 1984), at a unique sequence adjacent to FR, repeats that function in replication (Reisman et al., 1985) and transcriptional activation (Gahn and Sugden, 1995; Reisman and Sugden, 1986). Although we detected multiple RNAP II-binding sites, both at known transcription units and unannotated positions, none of these sites colocalized with CTCF.
High-resolution mapping of protein positions with ChIP-seq on small viral episomes yields advantages over similar experiments in larger genomes: technical gains allow confident data analysis and verified annotations help generate functional hypotheses of protein function. The high copy number of episomes, ~ 45 in the case of EBV-infected Raji cells (Sternas et al., 1990), increases the signal-to-noise ratio of ChIP experiments because of deeper sequencing compared to the simultaneously examined human genome. Multifold coverage of the background consequently allowed us to statistically define an experimental baseline for experiments. This simplified peak calling to choosing a suitable enrichment over background; our conservative threshold of a 10-fold enrichment yielded an ostensible false discovery rate of 0 and strong confidence in our identification of binding sites. Viral genomes have also been well annotated and subjected to functional mutagenesis experiments. Once protein-binding sites have been determined at high resolution, we can correlate occupancy with known and tested sequence elements. These advantages yield reliable distributions of occupancy that allow us to make functional inferences based on protein positions.
Widespread distribution of CTCF and cohesin on the EBV genome reveals binding at or near both latent and lytic genes, suggesting distinct regulatory functions at different positions. Focus on CTCF function in EBV has been limited to binding sites within the latency control region, and consequently potential roles for CTCF have only involved the determination of latent promoter choice and establishment of boundaries between latent and lytic genes (Chau et al., 2006; Day et al., 2007; Tempera et al., 2010). Our experiments identified CTCF-binding sites widely dispersed on the genome, including in and around many lytic genes. We also observed colocalization with cohesin, similar to what is observed in the human and KSHV genomes (Parelho et al., 2008; Stedman et al., 2008; Wendt et al., 2008), that is likewise not limited to areas of latent transcription initiation. Because of the widespread occupancy distributions, we surmise that CTCF and cohesin may be performing functions near lytic genes separate from those previously suggested in latency control regions.
RNAP II does not colocalize with CTCF, and RNAP II positioning suggests functions separate from the transcription of latent genes. We find RNAP II at the EBER transcripts, short non-coding RNA with expression predominantly driven by RNA Polymerase III but also partially transcribed by RNAP II (Kirchner et al., 1991). We also find RNAP II at two novel positions, sites that yield the strongest enrichment of occupancy in our experiments yet do not map to any annotated transcripts. The strongest signal maps to the unique sequence adjacent to FR within OriP, the proposed origin of replication for latency. The second strongest RNAP II signal maps upstream of the EBER-1 transcript and does not appear to correspond to an annotated element. We were unable, however, to find CTCF occupancy at any of these RNAP II-binding sites. We also could not detect RNAP II and cohesin colocalization as observed in the human genome (Kagey et al., 2010). RNAP II, like CTCF and cohesin, binds near different regulatory elements throughout the genome, thus suggesting functions ranging from transcription to replication.
RNAP II occupancy detects a subset of known transcription units in the EBV-infected Raji cells, but CTCF proximity correlates with both active and repressed promoters. The two EBER RNAs are actively transcribed (Lerner et al., 1981) and strong RNAP II enrichment is observed. A complex set of alternatively spliced mRNAs, referred to as BARTs, are generated from the rightward transcription of the BamHI A fragment (Chen et al., 1992; Sadler and Raab-Traub, 1995). Different initiation sites have been proposed, but we detect a weak signal in only one promoter (Smith et al., 1993). Similarly, conflicting evidence exists regarding the identity of the transcription start site (Tao et al., 1998; Woisetschlaeger et al., 1990) for the set of alternatively spliced mRNAs that yield the EBNA genes (Allday et al., 1988; Petti et al., 1988; Wang et al., 1987). Although the W promoter is obscured from our analysis by repeat regions, we find RNAP II at neither the C nor Q promoters. This cell line also expresses the LMP1 gene (Contreras-Brodin et al., 1991), as well as LMP2A but not LMP2B (Sample et al., 1989). Although weak RNAP II enrichment is observed in the general promoter region for these cells, the peak does not correlate to an annotated element. Combining our RNAP II localization with literature describing known transcription in Raji cells gives us the opportunity to compare CTCF occupancy with polymerase activity. Again, however, neither activity nor putative repression of promoters correlates with CTCF proximity. We interpret CTCF occupancy near both active and repressed promoters as another suggestion of context-dependent gene regulation.
Combining confident, high resolution mapping of protein positions by ChIP-seq and the deep annotation of viral genomes allows us to make specific and testable inferences. What functions can we hypothesize for CTCF, cohesion, and RNAP II? In addition to operating within the latency control region, CTCF and cohesin colocalization near lytic genes may control those regulatory elements, for example by repressing lytic transcription. In addition to generating latent transcripts, occupancy at the origin may couple RNAP II recruitment with replication. CTCF, cohesin, and RNAP II all share the common potential of context-dependent functions throughout the EBV genome.
EBV genome-positive Raji cells (Pulvertaft, 1964) (ATCC CCL-86) were maintained in RPMI-1640 media containing 25 mM HEPES and supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) β-mercaptoethanol. Antibodies used were anti-CTCF (Upstate, 07–729), anti-Rad21 (Bethyl Labs, A300-080A), and 4H8 (Abcam, ab5408).
1 × 107 Raji cells were used per ChIP-seq experiment. Crosslinking was performed by adding formaldehyde to 1% (v/v) at room temperature for 3, 10, and 5 min for CTCF, Rad21, and RNAP II immunoprecipitations, respectively. The reaction was quenched by incubation with 125 mM glycine for 10 min at 4 °C. Cells were pelleted at 1,000g for 5 min at room temperature and resuspended in 50 mM HEPES-KOH, 1 mM EDTA, 150 mM NaCl, 10% (v/v) glycerol, 0.5% (v/v) Triton X-100, Complete Protease Inhibitor Cocktail Tablets (Roche), pH 7.4, for 30 min on ice. Crude nuclei were collected by centrifugation at 600g for 5 min and lysed in 2 ml of 10 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, 5% (v/v) glycerol, 0.1% (w/v) sodium deoxycholate, 0.1%(w/v) SDS, 1% (v/v) Triton X-100, Complete Protease Inhibitor Cocktail Tablets (Roche), pH 8.0. DNA was sheared with a Bioruptor water bath sonicator (Diagenode) to produce fragments ~ 100–200 bp in size. A fraction of the sample was set aside as a genomic DNA input control and crosslinking was reversed in 10 mM Tris, 1 mM EDTA, 0.7% (w/v) SDS, 200 mg/ml proteinase K, pH 8.0, at 55 °C for 3 hrs followed by incubation at 65 °C overnight. DNA-protein complexes were purified using 10 μg epitope-specific antibodies and 100 μl protein G Dynabeads (Invitrogen) for 4 hrs at 4 °C and followed by consecutive washes, first in 10 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, 5% (v/v) glycerol, 0.1% (w/v) sodium deoxycholate, 0.1%(w/v) SDS, 1% (v/v) Triton X-100, Complete Protease Inhibitor Cocktail Tablets (Roche), pH 8.0, second in the same buffer with 500 mM NaCl, and last in 20 mM Tris-HCl, 1 mM EDTA, 250 mM LiCl, 0.5% (v/v) NP-40, 0.5% (w/v) sodium deoxycholate, Complete Protease Inhibitor Cocktail Tablets (Roche), pH 8.0. Complexes were eluted from the beads with 10 mM Tris, 1 mM EDTA, 0.7% (w/v) SDS, pH 8.0, and crosslinking was reversed as with the input control DNA.
ChIP-seq libraries were prepared by adaptor-mediated amplification (Robertson et al., 2007) with a few modifications. Both ChIP DNA and 10 ng of input DNA were repaired and phosphorylated with T4 DNA polymerase, Klenow polymerase, and T4 polynucleotide kinase. Double-stranded oligonucleotides based on genomic adapters (Illumina) were synthesized such that the product corresponded to the two adapters joined together at blunt ends and separated by deoxyuridine. After the addition of a 3′ adenine to the repaired DNA, samples were ligated overnight to the modified adapters in a 2:1 adapter:sample ratio. Ligation reactions were digested with uracil-DNA glycosylase for 30 min at 37 °C. Amplification of the DNA libraries was performed using PCR primers 1.1 and 1.2 (Illumina) for 18 cycles. The resulting PCR products were purified using Ampure beads (Agencourt). Size fractionation and purity of the finished ChIP-seq DNA libraries was monitored with a DNA 1000 Assay (Agilent). DNA concentrations were quantified using a combination of Quant-iT PicoGreen (Invitrogen) and UV absorbance to obtain a diluted final concentration of 10 nM. Clusters were generated on a Cluster Station (Illumina) and 36 cycles of sequencing performed with a Genome Analyzer II (Illumina). All ChIP-seq experiments were performed with independent replicates.
After ignoring the first basecall, 32 bp sequence fragments were obtained and mapped to the EBV reference genome (Genbank ID: NC_007605.1) using the Pipeline software (Illumina) allowing for up to 2 mismatches. Unique sequence tags were extracted and merged for each duplicate experiment. For the input, CTCF, cohesin, and RNAP II experiments, the combined data sets consisted of 3,164 plus 1,674, 11,937 plus 41,829, 4,474 plus 13,965, and 11,336 plus 7,292 sequences, respectively. Data was further processed with scripts written in AWK. Corresponding to the original DNA fragment size loaded onto the sequencer, the 32 bp tags were extended directionally by 100 bp and the number of hits per genome position counted. Each data set was then normalized to the internal baseline. Hits corresponding to deleted and repeat regions of the genome were first masked. The unmasked values were then sorted by count number and the mean and median values calculated. This set was culled in 1% increments, removing the highest values, until the calculated mean equaled less than the median. This process simulates removal of peaks from a data set until only a background remains. The last calculated mean is thus equivalent to the average number of hits within the background. All sequence hit counts were then divided by this value. This normalization results in expression of the reported peak heights relative to background. The selection criteria used for identifying a binding site were that each peak contain an approximately equal proportion of forward and reverse reads and that the maximum of each peak have a minimum ten-fold enrichment over background in both replicate experiments. Peaks coordinates are expressed as the full width at half maximum height.
We thank Clement S. Chu, the UCSF Center for Advanced Technology, and Hiten D. Madhani for access to instruments needed in deep sequencing experiments. Our research is supported by an institutional grant to JJ L. Miranda from the UCSF Fellows Program, which is funded in part by the UCSF Program for Breakthrough Biomedical Research and the Sandler Foundation. Samantha B. Cooper and Keith R. Yamamoto are supported by National Institutes of Health grant CA020535 to Keith R. Yamamoto.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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|Message view
|« Date » · « Thread »
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|RGB ES <rgb.m...@gmail.com>
|Re: Artists wanted (was Re: Crystal and Oxygen images)
|Sat, 12 Nov 2011 19:25:08 GMT
2011/11/12 Pedro Giffuni <firstname.lastname@example.com> > Hi; > > Yes it's very difficult, but it's one of those things > where people with different (non-programming) skills > can make a huge contribution. > > Pedro. > > --- On Sat, 11/12/11, FR web forum <firstname.lastname@example.com> wrote: > > > Hello, > > > > ----- Mail original ----- > > >This may be an interesting opportunity to get a new > > set > > >of icons. I looked around and there is a set of icons > > >under different licenses here, that may serve as a > > >starting point: > > Very difficult to create this set. > > > > Maybe having the same system than Firefox skin: > > https://issues.apache.org/ooo/show_bug.cgi?id=18829 > > > Sorry for the two previous (and empty) emails... rekonq made me a joke, it seems. The problem here is not to create the icon but to package them in a way AOOo can use: If you look inside the compressed file there are thousands of images and the naming convention is not easy to understand. Someone will need to explain how to build that icon set. On a side question... freedesktop defined a standard way to name icons: if AOOo change to that standard (yes, I know, it's not easy, but I'm speculating...) maybe everything will be more easy. Maybe it could be possible to use the icons provided by the desktop environment. This way AOOo will only need ONE fall-back theme to use when something is missing. Just my 2 crazy ¢. Cheers Ricardo
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Russian Orthodox Church in Great Britain and Ireland
(Patriarchate of Moscow)
Diocese of Sourozh
Русская Православная Церковь в Великобритании и Ирландии
(Московский Патриархат)
Сурожская Епархия
Welcome to the website of the
Parish of St. Peter and St. Paul.
The Parish of St. Peter and St. Paul
will meet to worship at;
St. Joseph R.C. Church,
Tangier Road,
Copnor Bridge,
Portsmouth, P03 6JH.
Next service :
Sunday, 17th July 2016
The Divine Liturgy of St John Chrysostom
Glorious Apostles St Peter
and St Paul
Patronal Feast
followed by celebration
* * * * * * * * * * * * * * *
Confessions are heard on Sundays before the Liturgy or by prior arrangement with the Priest.
For Baptisms, Weddings and Panikhidas (Memorials), you are invited to contact the Priest, at least three weeks in advance.
The Parish is part of the Diocese of Sourozh within the Patriarchate of Moscow.
The Divine Liturgy is served in English and Church Slavonic.
The parish follows the Old (Julian) Calendar.
We are a multi-ethnic parish. After the Liturgy we enjoy
a 'bring-and-share' meal together.
All are welcome !
Archpriest Michael Gogoleff Tel: 01225 858792
fax: 01225 852211
Parish Warden: Michael O'Shea, e-mail: email@example.com
Russian Orthodox Church of Great Britain and Ireland,
Diocese of Sourozh - www.portsmouthorthodoxchurch.org.uk
Troparion to St Peter and St Paul :
Leaders of the apostles and teachers of the world,
pray to the Master of all to grant peace to the world
and great mercy to our souls.
Tone 4


text:
Philadelphia, PAUSA
Hometown: Allentown,moved many places since
September 11, 2012
Events in Philadelphia as opposed to the suburbs.
Relaxing during the week and meeting new people from different backgrounds.
No answer yet
Hi,I'm Ilene originally from Allentown by way of many places,the last being Utah.What I could tell you over a drink! No,just kidding. kinda sorta... Always love to meet new people and find new venues.
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Nightly Standard performs at Third Thursdays, WYEP's local music happy hour on January 16, 2014.
Set List:
Bye Bye
Our Days
All of You
Off the Beaten Track
Not Meant to Be
Hold Them Above
Nothin' to Say
I Just Wanna Make Love to You
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