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Thioredoxin glutathione reductase (TGR) is a member of the mammalian thioredoxin reductase family that has a monothiol glutaredoxin (Grx) domain attached to the thioredoxin reductase module. Here, we report a structure of the Grx domain of mouse TGR, determined through high resolution NMR spectroscopy to the final backbone RMSD value of 0.48±0.10 Å. The structure represents a sandwich-like molecule composed of a four stranded β-sheet flanked by five α–helixes, with the CxxS active motif located on the catalytic loop. We structurally characterized the glutathione-binding site in the protein and describe sequence and structural relationships of the domain with glutaredoxins. The structure illuminates a key functional center that evolved in mammalian TGRs to act in thiol-disulfide reactions. Our study allows us to hypothesize that Cys105 might be functionally relevant for TGR catalysis. In addition, the data suggest that the N-terminus of Grx acts as a possible regulatory signal also protecting the protein active site from unwanted interactions in cellular cytosol.
Citation: Dobrovolska O, Shumilina E, Gladyshev VN, Dikiy A (2012) Structural Analysis of Glutaredoxin Domain of Mus musculus Thioredoxin Glutathione Reductase. PLoS ONE 7(12): e52914. doi:10.1371/journal.pone.0052914
Editor: Michael Massiah, George Washington University, United States of America
Received: October 12, 2012; Accepted: November 22, 2012; Published: December 26, 2012
Copyright: © 2012 Dobrovolska et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: AD acknowledges the support from NT Faculty, NTNU. ES acknowledges the NT Faculty, NTNU, for financial support through a post-doctoral fellowship. OD acknowledges the NT Faculty, NTNU, for financial support through PhD fellowship. This work was also supported by United States National Institutes of Health GM065204. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Thioredoxin (Trx) and glutaredoxin (Grx) systems are two major thiol pathways that control cellular redox homeostasis [1]. The Trx system is composed of thioredoxin reductase (TR), thioredoxin (Trx) and Trx peroxidase, whereas the Grx system consists of glutathione reductase (GR), glutathione (γ-Glu-Cys-Gly tripeptide; GSH), glutaredoxin (Grx) and glutathione peroxidase (GPx). In these systems, electron flow is directed from NADPH through GR and TR towards their respective protein substrates. GR and TR belong to the pyridine nucleotide-disulfide oxidoreductase family. They are homodimers and contain a tightly bound FAD molecule in each subunit [1]. Mammalian TR and GR were found to be structurally and functionally similar, although TR has an additional C-terminal selenocysteine-containing active site, which serves as a substrate for the N-terminal active site [2], [3], [4], [5], [6], [7].
Three TRs genes have been identified in humans, including TXNRD1 (cytosolic TR, TR1), TXNRD2 (mitochondrial TR, TR3) and TXNRD3 (thioredoxin glutathione reductase, TGR) [8], [9], [10], [11], [12], [13]. TGR is unusual among TRs in that it has an additional N-terminal Grx domain, which is fused to a canonical TR module [13], [14], [15], [16]. The amino acid sequence of the TR module of TGR is more closely related to TR1 than to TR3 [13] and its Grx domain has a monothiol CPHS catalytic motif [13], [14], [15], [17]. The active site motif of the Grx domain of TGR can receive electrons from either the TR module or from GSH, and the protein was proposed to function predominantly in disulfide bond formation and isomerization in sperm proteins during spermatogenesis [14]. Mammalian TGR exhibits broad substrate specificity and can reduce various components of both Trx and Grx systems [16]. In particular, it was demonstrated that TGR can catalyze reactions associated with Grx (deglutathionylation), GR (NADPH-dependent reduction of GSSG) and TR (NADPH-dependent reduction of Trx) activities. It was argued that Grx and GR activities of TGR are mediated by its Grx domain [16].
Structural characterization of proteins is an essential step for establishment of their functional peculiarities. Structures of platyhelminth TGR (pdb code 2V6O) and the Grx domain of human TGR (pdb code 3H8Q) have been recently determined. We previously reported NMR resonance assignments of full-length and shortened (lacking 22 N-terminal amino acids) forms of the Grx domain of Mus musculus TGR [18]. In the present work, we report solution structure of this Grx domain using high-resolution NMR spectroscopy. This Trx-fold structure validates the model of the Grx domain [19] and is consistent with the structures of other Grx. We further used the structure to carry out comparative sequence, structure and charge distribution analyses of Grx and Grx domains in order to explain structural and functional peculiarities of the TGR's Grx domain.
Materials and Methods
Reduced glutathione (GSH) was purchased from Acros Organics, and oxidized glutathione (GSSG) from Sigma Aldrich. Both compounds were dissolved in a buffer containing 10 mM sodium phosphate, 10 mM NaCl, pH 7.5.
Sample preparation
Protein expression and purification of a uniformly isotope labeled (15N/13C) His-tagged version of the full-length and shortened forms of the Grx domain of mouse TGR (hereafter Grx and sGrx, respectively) was carried out as described previously [18]. NMR samples of reduced 1 mM Grx or sGrx in 10 mM sodium phosphate buffer, 10 mM NaCl, 10 mM β-mercaptoethanol, in 95% H2O/5% D2O and 100% D2O, pH 7.5, were analyzed by NMR.
NMR spectroscopy
NMR spectra were recorded at 298 K on a Bruker Avance 600 MHz spectrometer equipped with a 5-mm z-gradient TXI (H/C/N) cryoprobe. Three-dimensional 13C- and 15N-edited 1H Nuclear Overhauser Effect Spectroscopy (NOESY) spectra were recorded in D2O and H2O, respectively. NMR data were processed using Bruker XWinNMR, version 3.5. NMR spectral analysis was performed using CARA version 184.108.40.206.
Structure calculation
NOE cross-peaks were identified, assigned and integrated in the aforementioned NOESY spectra using the CARA program. The CALIBA subroutine in CYANA 2.1 was used to convert cross peak intensities to distance constraints. Dihedral angle constraints were derived from secondary chemical shifts using the TALOS program [20]. Based on the input, the structure was calculated using the torsion angle dynamics program CYANA2.1 [21]. Twenty conformers with the lowest final CYANA target function values were further energy minimized in vacuum using AMBER force field with the aid of AMBER 9 program [22]. The mean structure was generated using MOLMOL 2k.2.0 [23] and further energy minimized in AMBER.
Structure analysis
Quality of structures was analyzed using MOLMOL and PROCHECK - NMR [24]. The relevant figures and electrostatic potentials were prepared using MOLMOL version 2k.2.0.
NMR experiments
In order to characterize the glutathione-binding site of Grx NMR 15N-1H HSQC titration experiments were performed. A 1 mM sample of 15N-labeled Grx in buffer, containing 10 mM sodium phosphate, 10 mM NaCl, pH 7.5, was titrated with unlabeled GSH and GSSG at room temperature in the following proportions: 1∶1/3; 1∶1/2; 1∶2/3; 1∶1; 1∶2; 1∶10, either in the absence or presence of 10 mM β-mercaptoethanol. In the membrane environmental modeling experiment, 5% w/v SDS was added to the solution of 15N-labeled Grx and the 15N-1H HSQC spectra of the mixture were recorded at 30°C and 42°C. For water exchange experiments, samples of 15N-labeled Grx or sGrx in the NMR buffer were lyophilized and further dissolved in D2O. A course of subsequent 15N-1H HSQC spectra for each protein was recorded every 30 min.
Bioinformatics analysis
Protein multiple sequence alignments were performed with ClustalW [25]. Sequence similarity analysis was performed by the SIAS server (http://imed.med.ucm.es/Tools/sias.html). Structural superimposition was carried out using SuperPose [26]. An analysis of the N-terminal region (residues 1–22) was performed using iPSORT [27] and MITOPROT [28].
Results and Discussion
Structure description
Solution structure of the Grx domain of mouse TGR was calculated based on the NOE-derived geometrical constraints and dihedral angles obtained from TALOS. The geometrical constraints used in the calculations are summarized in Table 1. In total, 894 NOE-based upper distance limits and 182 ψ and φ torsion angle restraints were used to derive the Grx structure. The resulting Grx family was further energy-minimized. The geometrical constraints and coordinate files of the minimized Grx family were deposited in the PDB under the code 2lv3. Figure 1 shows a superimposition of the final 20 minimized conformers with the lowest target function, together with a ribbon representation of the minimized conformer closest to the mean structure showing the secondary structure elements, active site cysteine C48, and C-terminal C105 (further discussed). The calculated structure is of high quality and fully corresponds to the experimentally determined constraints.
Left: overview of backbone superimposition of 20 conformers with the lowest target function. Right: overview of the ribbon representation of the minimized conformer closest to the mean structure. The figure also shows the active site cysteine (C48) and C-terminal C105.
The N-terminal region of Grx (first 22 amino acids) was excluded from the structure calculation as most of the corresponding HSQC and NOE signals were not detected [18]; hence, the structure of Grx starts with Ala 23. Analysis of the structure shows that the Grx domain is a compact Trx-like spherical molecule with a central core of four-stranded β-sheets flanked on either side by five α-helices arranged in the order α1-β1-α2-β2-α3-β3-β4-α4-α5 (Figure 1). The N-terminal region begins with an α1 (residues Arg 24 - Glu 36), followed by β1 consisting of residues Val 40 to Ser 44. The active site Cys 48 - Ser 51 (-CPHS- motif) is situated on the unstructured loop between β1 and α2 (residues Arg 53–Ser 59). The strand β2 comprises residues Asn 66 to Glu 69; following a loop, α3 consists of residues Gly 76 to Ser 87, followed by β3 (Asn 94–Val 97) and β4 (Val 100–Gly 103). The C-terminal region includes α4 (residues Arg 107–Asn 114) and α5 (residues Leu 116–Leu 120), connected through a hinge section. Strands β1 and β2 are parallel, and strand β3 is antiparallel with β1 and β4. Helices α1 and α3 pack on one side of the β-sheet, whereas α2, α4 and α5 are on the other. Packing of the sandwich-like architecture is mainly maintained by hydrophobic interactions between the sheet and helices. The determined Grx structure shares significant structural similarity with the modelled Grx domain of mouse TGR [19].
N-terminal region
As mentioned above, the N-terminal region of Grx was not detectable in HSQC and NOE spectra. The absence of the corresponding cross-peaks could be attributed to the higher mobility of this protein region. A decrease in temperature may slow down protein mobility and thus allow detecting the missing cross-peaks. Nevertheless, the 15N-1H HSQC spectra of Grx recorded at a lower temperature (8°C) did not show additional signals in the spectra (data not shown). Further analysis of the first 22 amino acids of Grx using iPSORT suggested the presence of a candidate mitochondrial targeting peptide. Indeed, the N-terminal sequence is rich in positively charged and hydrophobic residues that may constitute the targeting helix [29]. Analysis of the N-terminus performed by MITOPROT predicted the cleavage site after the first 19 residues (MSSPPGRRARLASPGTSRP). However, analysis of cellular distribution of TGR suggested that the enzyme occurs in the cytosol of spermatids at the time of mitochondrial sheath formation [14]. In these spermatids, TGR is accumulated near the site of mitochondrial sheath assembly. It was shown that mammalian sperm is stabilized by disulfide bond (S-S) bridges cross-linking thiol-rich proteins present in the membranes of sperm mitochondria [30]. Thus, TGR, which combines the elements of Grx and Trx systems, might be involved in disulfide bond formation during spermatogenesis. The N-terminus can also act as a regulatory sequence. Showing little structural organization in solution, mitochondrial targeting sequences are predicted to form amphipathic α-helixes in the membrane or membrane-like environment. The amphipathic nature of these structures is thought to be important for their specific recognition by the protein import machinery [31]. A membrane-like media are prepared by self-association of surfactants in aqueous solutions, which are divided into two large groups: detergents (form micelles) and lipids (form bilayers) [32]. For preparation of micelles, which are widely used in NMR structural studies, negatively charged SDS detergent is often used [33]. In NMR spectroscopy, the formation of the protein's secondary structure results in the appearance of a set of well-dispersed HSQC cross-peaks. To further examine the N-terminal part of Grx, we recorded 15N-1H HSQC spectra of the SDS-treated Grx domain at 30°C and 42°C [34]. The obtained 15N-1H HSQC pattern was shifted towards the lower field of both dimensions; however, it did not change dramatically, and, as expected, contained additional well-dispersed cross-peaks, as shown in Figure 2. The number of new cross-peaks corresponded to (but did not exceed that of) the number of amino acids constituting the N-terminal segment, therefore, confirming that the positively charged N-terminus becomes structured in the negatively charged environment.
Green shows Grx HSQC spectrum at 30°C, light blue shows Grx HSQC spectrum in the presence of SDS at 30°C, and black corresponds to Grx HSQC spectrum in the presence of SDS at 42°C. For more details see the text.
Although the 1H-15N HSQC patterns of Grx and sGrx resemble each other, indicating overall structural correspondence of the two protein forms, several differences between them were observed. First, the full-length protein had a higher stability than the shortened form. Second, the signals in 15N-1H HSQC, corresponding to residues C105 and D106, were not found in Grx, while they were present and assigned in sGrx [18]. Weak HSQC signals and any NOESY patterns were also found for residues D74 and A77 in the corresponding spectra of Grx [18]. These findings correlating with the presence/absence of the N-terminus further highlight structural differences between the two protein forms (see the following paragraphs).
Electrostatic potential
Figure 3 shows electrostatic potential calculated for the obtained structure of Grx. It is apparent from the analysis of this figure, that the missing NOE signals of D74, A77, C105, D106 (see above) amino acids belong to the negatively charged region closest to the N-terminus. As the N-terminal region of Grx is composed of positively charged (R7, R8, R10, R18) and polar (S2, S3, S13, T16, S17, S20, S21) amino acids, their involvement in electrostatic interaction with negatively charged protein surface can be suggested. The broadening of the NMR signals corresponding to D74, A77, C105, D106 due to this interaction may be a reason that the mentioned amino acid signals in the NMR spectra of the full length form of Grx where not detected. Indeed, the resonances belonging to D74, A77, C105, D106, not observed in Grx protein, were firmly detected in sGrx lacking the mentioned electrostatic interaction. Interestingly, the active site motif of Grx (C48, P49, H50, S51), as monitored by our structural studies, resides on the neighbouring loop near residues C105 and D106. Thus, the N-terminal region, positioned in proximity to the active site, could shield it from the solvent and, therefore, protect from unwanted reactions stabilizing the full-length protein.
D2O exchange experiments
To further comparatively characterize both Grx and sGrx proteins, we carried out water exchange experiments monitoring behaviour of 15N-1H HSQC spectra in D2O for both full-length and shortened forms of the 15N-labeled Grx domain of TGR. Interestingly, during the first 30 minutes in D2O, Grx exchanged ten residues more with respect to sGrx (D33, G37, N38, V40, S59, V63, D71, Q72, E85, T108). However, after 3 hours of incubation in D2O, both Grx and sGrx reveal an identical pattern of exchanged/not exchanged residues. Therefore, our experiments show that while the final rate of water exchange is the same both for Grx and sGrx, the short term dynamics of the water exchange is different for these proteins. The observed differences mostly regard residues belonging to the negative patch involved in the suggested interaction with the positively charged N-terminus (see above). The fact that these residues exchanged within the first 30 min in Grx, while in sGrx they exchanged only 3 hours later indicates that the N-terminus in some way promotes faster rates of water diffusion into Grx protein.
Comparative sequence analysis of Grx
Grx occur in the majority of organisms in the three domains of life. Structures of many of these proteins were determined either by NMR or X-ray crystallography. Two main groups of Grx can be distinguished based on phylogeny, active site motifs, and domain structure: (i) 'classical' dithiol Grx containing the active site consensus sequence Cys-X-X-Cys (i.e., two Cys separated by two other amino acids); and (ii) monothiol Grx with a Cys-X-X-Ser active site consensus sequence. The latter Grx utilize only the N-terminal active site Cys in their catalytic mechanism, which is used together with two glutathione molecules, while dithiol Grx can use either one or both Cys in the active site. Both types of disulfides formed during Grx catalysis are reduced in vitro by GSH or TRs [35].
We analyzed an alignment of the Grx domain of mouse TGR with both Grx and Grx domains of TGR from various organisms, which contain mono- or dithiol active sites (Figure 4). The active site residues (highlighted with a red rectangle), and residues involved in the interaction with GSH (marked with black rectangles) are conserved in mono- and dithiol Grx, including the Grx of mouse TGR [36].
The active site residues of Grx are highlighted with a red rectangle, and the residues involved in the interaction with glutathione are within black rectangles. Positive and negative charged amino acids are marked with blue and red colour, respectively. Cysteines residues are marked in green. Sequences abbreviation: HS, Homo sapiens; SM, Schistosoma mansoni; XL, Xenopus laevis; MM, Mus musculus; DR, Danio rerio; EC, Escherichia coli.
Since interaction of the Grx domain with glutathione is assisted by electrostatic interactions, we next analysed the distribution of charged amino acids. As shown in the figure 4, the active site of Grx is surrounded by positively charged amino acids (marked in blue). Interestingly, only in E. coli Grx3 and the Grx domain of Xenopus laevis TGR, negative residues are found in the vicinity of the active site (Fig. 5, marked in red). It was suggested that these negative residues influence the redox potential of these proteins [37]. In addition, the C-terminal segment of the Grx domain of TGR from human, mouse, Danio rerio, Xenopus laevis and Grx3 from E. coli harbor an additional Cys residue. Our structural data indicate that the distance between the active site cysteine (Cys 48) and C-terminal Cys105 (Figure 1, right panel) is 10–15 Å. Although the distance might be too large for the formation of an intra-molecular disulfide bridge, according to our data there is no steric hindrance between these two cysteines located on unstructured loops. It can be hypothesised that the formation of an intra-molecular disulfide bridge between the two cysteine residues (e.g., as observed in mammalian MsrB1 protein [38], [39]) may have functional and, perhaps, even catalytic relevance.
Green corresponds to free Grx and magenta to Grx incubated with GSH/GSSG. Only the residues for which alteration of NMR parameters upon titration was observed are marked. Panel C: qualitative representation of the data. Solid and dashed horizontal lines below the Grx amino acid sequence highlight the residues interacting with GSH and GSSG, respectively.The N-term of Grx is marked in blue. For more details see the text.
Structural comparison with other Grx
Our analysis, as well as other structural studies of a set of GSH-dependent Grx [26] revealed common elements present in their binding sites: i) a CXXC/S active site motif; ii) a Tyr or a Phe in close proximity to the catalytic Cys; iii) a TVP motif with Pro in the cis conformation; iv) a GG kink in proximity to the active site; and v) conservation of charged residues at both edges of the substrate binding groove (GSH binding pocket). The structure of the Grx domain of mouse TGR was generally similar to other Grx structures, but varied in secondary structure elements attached to a common Grx core. For example, an N-terminal α-helix (α1 in our structure) was present in many Grx including human TGR (PDB code 3H8Q), human Grx2 (PDB code 2FLS), Grx of SmTGR (PDB code 2V6O), monothiol E. coli Grx4 (PDB code 1YKA), and poplar Grx C1 (PDB code 2E7P), but not in the dithiol E. coli Grx3 (PDB code 1FOV). A lack of C- and/or N-terminal α-helices together with the length of the loop preceding the active site motif, was suggested to play a key role in constraining the degree of conformational adaptability for substrate binding displayed by Grx [40]. Minor structural differences were also observed between the Grx domain of mouse TGR and the Grx domain of SmTGR: the latter was characterized by a shorter C-terminal α-helix [41].
Recent studies [42] demonstrated that SmTGR may function via two catalytic mechanisms: monothiol and dithiol. In the monothiol mechanism, when the GSH concentration is high, glutathionylated catalytic Cys (Cys28) of Grx gets resolved by GSH. At low GSH concentrations, a second, dithiol mechanism applies, wherein the C-terminal Cys (Cys31) in the CXXC motif acts as a resolving group, breaking the disulfide bond between Cys28 and GSH, forming an internal Cys28-Cys31 disulfide and releasing GSH. An oxidized Grx can be further reduced by the redox-active Cys/Sec–Cys pair of the TR domain [43]. The same study analyzed the deglutathionylation activities of a SmTGR variant, in which Cys31 was replaced with Ser (making it analogous to mouse TGR), which exhibited 22% of wild type SmTGR activity. This study suggested that the role of the second Cys in the monothiol mechanism is to stabilize the thiolate anion of the N-terminal Cys through a hydrogen bond, thus facilitating its nucleophilic attack on GSSG.
Grx titration with GSH/GSSG
Figure 5 illustrates fragments of the 15N-1H HSQC titration of the Grx domain with reduced (Figure 5A) and oxidized (Figure 5B) glutathione. Upon interaction with these two molecules the chemical environment of the nuclei involved in the interaction changes, which results in perturbation of the corresponding NMR signal: i.e., chemical shift change or signal broadening occurs. By performing NMR titration of 15N-labeled Grx with its unlabelled partners, GSH and GSSG, we monitored their interaction and mapped the Grx residues involved in binding with the respective partners.
Upon titration of Grx with either GSH or GSSG, nearly 90% of the signals remained unaltered; however, some of the signals appear changed (mostly broadened). The titration experiments of the Grx domain titration with GSH revealed signal broadening that corresponded to the following amino acids: S44, K45, C48, P49, H50, S51, T52, R53, V54, E81, T91, V92, P93, N94, G103, G104, R107. Grx titration with GSSG showed changes for the following residues: N38, K45, C48, P49, H50, S51, T52, R53, V54, E81, T91, V92, P93, N94, G103, G104, and R107 (Figure 5C). Interestingly, amino acids observed in the two experiments almost coincided. These experimental data not only point to the glutathione binding site in the Grx domain of TGR, but also suggest that the binding sites for reduced and oxidized glutathione largely overlap.
This study describes the NMR solution structure of the monothiol Grx domain of mouse TGR. As expected, the protein possesses a Trx fold and consists of a four-stranded β-sheet flanked by five α-helices. The active site motif containing the catalytic redox-active Cys is located on the protruding loop connecting strand β1 and α2.
Analysis of the N-terminal segment of Grx, which was not included in the structure determination, showed that it has features of a targeting sequence or a regulatory region. It was found, by analyzing 15N-1H HSQC spectra, that this segment becomes structured when protein is treated with a detergent, thereby mimicking membrane-like environment. Based on the analysis of surface charge distribution of the protein, we suggest that the N-terminus resides near the active site, shielding it from redox interactions.
Sequence alignment of the domains with other Grx and Grx domains revealed a characteristic GSH-binding site, which was further characterized with the help of NMR. The data suggest a significant overlap between the GSH and GSSG binding sites. Further analysis of mammalian TGR function would require structural information of the entire enzyme.
We gratefully acknowledge support from BioStruct, the Norwegian national graduate school in Structural biology.
Author Contributions
Conceived and designed the experiments: ES AD. Performed the experiments: OD ES AD. Analyzed the data: OD ES VG AD. Wrote the paper: OD ES VG AD.
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TazendaVer en iTunes
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Italian experimental rock group Tazenda started as a threesome in 1987, making their debut with a song called "A Sa Zente." A year later, the band released a self-titled album, followed by 1991's Murales, went on tour around the country, and even had the opportunity to become an opening act for Simple Minds. In addition, Tazenda teamed up with singer/songwriter Pierangelo Bertoli to participate in San Remo's Festival with a song called "Spunta la Luna Dal Monte," returning to the same event in 1992 with "Pitzinnos in Sa Gherra" and joining a concert organized by Amnesty International and the Italian-Tibetan Society. After Limba, the alternative group released a live album called Il Popolo Rock in 1993 and Fortza Paris in 1995. They recorded Sole Di Tazenda in 1997, Sardinia in 1998, and Bios — Live in Ziqqurat in 2001.
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Live Trane: The European Tours 1961-1963 Box set, Live, Original recording remastered
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Not just live Trane live PRIME Trane, for this 7-disc set takes its 37 performances from the three early '60s European tours Norman Granz produced under the auspices of his Jazz at the Philharmonic series. Over half of the selections here are unreleased, representing the biggest cache of newly discovered Trane in quite a while. Newly remastered, with notes wait till you hear the FIVE different versions of My Favorite Things !
For Coltrane fanatics, Live Trane represents another part of the endless jigsaw of recordings from live sources that have proliferated since his death. For the average jazz fan, it's a concentrated dose that gives a European dimension to the Impulse documentation of what the great man was doing with his working band live in the US in the early 1960s. All seven CDs are drawn chronologically from three tours Coltrane made of western Europe under the auspices of Norman Granz between November 1961 and November 1963: the recordings gather together and correctly identify (for the first time) the dates and locations of all the previous Pablo releases of this material. (Granz had no interest in such anorak activities). There is four hours of material culled from Granz's tapes of the tours that Pablo held back before this. Quite a lot of it has been floating around on bootleg LPs and CDs for years, but there are genuine discoveries--for example, the 25 November appearance in Hamburg (in poor sound, by the way, with Tyner on an out of tune piano) is even listed by the indefatigable Coltrane discographer Yashuro Fujioka as "three long titles, details unknown", with no indication that the concert was even recorded. A November 17, 1962 Paris appearance that is well represented here is almost as undocumented. So there's plenty of rare Coltrane to feast your ears on here, plus a fair sampling across two CDs of 1961-vintage Eric Dolphy. However, there is still unreleased music from the same concerts, and the remastering has often opted for a reverb-dominated sound and occasionally makes the music sound ghostly and diffused. This is an important document, then, but not a perfect one. --Keith Shadwick
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Backed by McCoy Tyner,piano,Eric Dolphy,alto sax,flute,bass clarinet,Reggie Workman or Jimmy Garrison,bass,and the prodigious Elvin Jones on drums,Trane plays incendiary choruses on his actual repertoire: "my favorite things","Mr P.C.","impressions","Naima","chasin' the Trane","the promise"...
The intensity of this music is such than it's very difficult to find words to describe it.Of course,you'll listen to five versions of "my favorite things",or "impressions",or "Mr P.C.",four of "Naima",but you won't be bored;each version is so different from the other ones that you'll find treasures in each one.There is also the most magnificent version of "Spiritual" Coltrane ever recorded;a somptuous version of "Afro blue";the haunting "Lonnie's lament",one of Trane's most beautiful tunes;and hours and hours of some of the most joyful,sensual,essential music of the past century.This 7 CD set is one of the most important issues of the past decade,and an essential milestone in JOohn Coltrane's recorded works.Beeing a Coltrane addict,it seems impossible to miss it.Here are hours of treasures,hours of one of the most fascinating music of all times.
The recordings here are technically not up to the Impulse standard, being all mono and of varying (though acceptable) quality. There is the occasional offmike blowing by Coltrane, and on some tracks (notably cd 4 and 5) one of the musicians' (Garrison?) hideous groaning almost drowns out the music. Still, these are minor irritations considering the importance of these recordings.
The repertoire is well familiar to Coltrane fans, and the differences between the various versions provide fascinating insights into the art of improvisation at the highest level. There are a few slightly uninspired performances here, but others belong among the finest Coltrane recordings ever, and there is some stuff here you won't find anywhere else.
It should be noted that experts have pointed out some serious discograhical errors in the cover notes (some of the recordings seem to actually stem from Birdland, NYC).
But what was John Coltrane, really - what was great about him? Well, he was a great soloist; I would argue that the length and rigor of his solos from about 1957 on make him THE MAN in this regard (with apologies to Bird, Miles, and Jimi among others). He was a great player generally, with a supremely confident tone who could play the blues unlike anyone else has. And from roughly 1960-63 he was a great bandleader, taking his famous quartet deep into the heart of African-American (as well as other) forms and coming out a winner every time.
All of those attributes are on display in this collection of music. There are other documents of Coltrane's quartet live (most notably the 1961 Village Vanguard recordings) but I think this is the best place to really catch how great these guys were. These recordings are from tapes made by concert promoter Norman Granz - and some were issued before on his Pablo label (as "Afro-Blue Impressions", "Bye Bye Blackbird", and "The Paris Concert"). Those were great records, records that showed this band playing at length and brilliantly. This collection is the same thing, times 3 or so (it's about 3 times as much material as we had before).
Any complaints about duplication in the repertoire, or so forth, miss the primary point of importance here. Which is that this is some of the best music ever made or recorded, and unlike the rest of Trane's recorded legacy. It's just fantastic stuff.
Most recent customer reviews
The music on this cd is just an incredible representation of the intensity that Coltrane brought to the bandstand. Read morePublished on Oct. 31 2003 by Zachary Lipton
I was glad to add these live sets to my Coltrane collection. The sound is good and the playing is excellent. Highly recommended.Published on Dec 17 2001 by Brian "Jazz Fan" J. B.
Yes, this is monophonic and has a rather distant and uneven sound. No, this doesn't matter much to the Trane Tribe, who cannot get enough of the classic quartet's exotic... Read morePublished on Dec 12 2001 by Douglas Groothuis
Historically this live cd falls within the most productive period of John William Coltrane's artistry. Read morePublished on Dec 10 2001 by scott mcdowell
Not as good as the 4-CD-boxset of the Village Vanguard Concerts, this latest one still deserves not less than 5 stars. Read morePublished on Nov. 27 2001 by Aydin Erer
I have a ton of Coltrane's discs&of course the whole Village Vanguard Set which is far Better to Me than this Compilation. Read morePublished on Nov. 3 2001 by MAXIMILLIAN MUHAMMAD
This doesn't come close in terms of quality or performance to any of the sets from Impulse but it is nonetheless an important release. Read morePublished on Oct. 29 2001 by A. Johnston
This box earns four stars (and should earn five) simply for the quality of the performances. On stage, the classic Coltrane quartet stretched the tunes out, often to more than... Read morePublished on Oct. 25 2001 by Dan Plankton
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text: Library Hours: Mon, Wed, Thurs 10:00-8:00 Tues, Fri 10:00-6:00 Sat* 10:00-4:30 *Open Saturdays from Labor Day to Memorial Day LIBRARY CATALOG ebooks/audiobooks Ask a Librarian Library News Calendar Services Upcoming Events Aug 1 Mon 2:00 pm Caricatures Workshop by Gina Min... Caricatures Workshop by Gina Min... Aug 1 @ 2:00 pm – 3:00 pm Come see Gina Minichino an artist who will teach and draw caricatures. Ages 5-13. Registration required. Limited space. 6:30 pm Pajama Storytime & Bring Your St... Pajama Storytime & Bring Your St... Aug 1 @ 6:30 pm – 7:00 pm Come in your pajamas. Bring your favorite stuffed animal & listen to stories!! Aug 2 Tue 11:00 am Beginner's Crochet ( Ages 9-14) ... Beginner's Crochet ( Ages 9-14) ... Aug 2 @ 11:00 am – 12:00 pm Interested in Crocheting? Come to our Beginner's Crocheting class for ages 9-14. Registration required. Limited space. Aug 3 Wed 3:30 pm Zumba for Kids (ages 5-13) : Reg... Zumba for Kids (ages 5-13) : Reg... Aug 3 @ 3:30 pm – 4:30 pm Zumba for Kids. Come shake and move your body to groovy music. Ages 5-13. Register for each class. Limited Space View Calendar On-going Services and Activities wifi-smchess-smslidesmg-afterschoolsmg-largeprintsmg-resume-helpsmg-daily-newspapers smg-interlibraryloanssmg-compclassesstorytime-smsldesmg-driveredslide National Parks and Recreation Month Union County Parks and Recreation NJ State Parks, Forests, Recreation Areas and Marinas National Recreation and Park Association National Ice Cream Month About National Ice Cream Month The History of Ice Cream Fun Facts about Ice Cream Signup to receive Library News Name Email Address 2 + 1 = Submit


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The Belmont TavernRead MoreSpice up your week with a trip to Belmont Tavern in Milwaukee, and enjoy grub at the top of its game. Enjoy the cool summer breezes on Belmont Tavern's seasonally available outdoor seating. Music lovers will appreciate Belmont Tavern's freshly mixed tunes spun by live DJs. Take note that the restaurant can get a bit loud, so vocal cords and eardrums should be in tip-top shape. The restaurant fills up on the weekends, so keep that in mind before heading out. Belmont Tavern patrons can find street parking at the N Jefferson St location. Belmont Tavern's diners can store their bikes safely at the rack around the corner.784 North Jefferson StreetMilwaukee, WI US
Mikey'sRead MoreMikey's is serving up American favorites with a tasty tweak. If gluten is something you try to avoid, check out the G-free menu at Mikey's. Low-fat fare is also available for those keeping an eye on their diet. Enjoy a drink with your dinner — this restaurant has a full bar to serve up a glass of wine, beer, or more. Load up the mini-van and bring the kids to this restaurant — they'll love the menu and scene here as much as mom and dad. Looking to host a party but don't have the space at home? You'll love the private room offered at Mikey's — just right for large and merry gatherings. At Mikey's, the prime seating is on the patio. Come check out what all the buzz is about. Volume levels at the restaurant can approach ear-splitting levels between the noisy crowds and the booming music. Shake off the stiff workday duds at Mikey's — attire is casual. You can also grab your food to go. Street parking and valet parking options are located around the corner from Mikey's. For a decently-priced meal that s not too fancy, Mikey's hits the nail on the head. At Mikey's, you can pay with any major credit card. For a dish just like mom made, you'll definitely want to stop by Mikey's' tasty restaurant. Make your way over to the highly-rated Mikey's and taste your way through some great American dishes. When you're feeling hungry, come satisfy your hunger with some tasty and trendy American eats from Mikey's.811 North Jefferson StreetMilwaukee, WI US
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This is a great sign for Atlantic City. The Revel crane - which has towered over the building and the city for how long? - is being lowered. Its work is finally done. When you consider how it once looked like this building would loom over the resort half-finished for years, bringing down this crane is a major milestone. (And it also means the crane contractor got paid.)
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Slots have got the be the most commonly used features of any casino. Anyone that goes into casino's if you ask them if they go on the slots, will answer yes. They usually go on them as they are the easiest games to play, you don't really have to think and they are a game of luck but there are still a few things you should know to get you winning those $1000's.
There are many different types of slots but most are used by inserting money in the slot (not if your using an online casino) and pressing a button or pulling a handle. Also, some slot machines have 3 reels and some have 5 reels or maybe even more. The jackpots are usually higher on the machines that have 5 or more reels as to get the jackpot you have to get a certain combination which will be harder with 5 reels.


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After doing a project level find the lower pane displays the text: "No Editor" and nothing else. This just started happening a couple of days ago. I had updated to the latest iPhone Xcode build, this may have had something to do with it.
I just deleted the Developer directory and did a re-install of Xcode 3.2.1. that did not help.
How an I get this to work again?


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Art Print by Vincent Van Gogh | #412895
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Sunflowers by Vincent Van Gogh
Sunflowers by Vincent Van Gogh
Sunflowers by Vincent Van Gogh


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tag:blogger.com,1999:blog-7688526164999265223.comments2016-07-17T19:05:48.156-07:00Jenny MatlockJennyhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-66421715728350633962016-07-17T19:05:48.156-07:002016-07-17T19:05:48.156-07:00Oh Jenny, my heart goes out to you. Though my blog...Oh Jenny, my heart goes out to you. Though my blogging is so sporadic, you have always been one that I'd stop by to visit. You were around when I first started some 7 1/2 years ago. You have been one to understand the depth of a few emotional posts I've written and have let me know.

I don't know what you're going through, but pray that medicine and miracles will work together to restore your mind and body.

I will continue to stop here when my blogging spurts happen, to see if you've updated.

May god bless you and your family.

ps. Love your infectious smile! And thanks for suggesting an awareness of others who might be experiencing pain.Anitahttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-90772135202071569252016-06-29T04:46:49.016-07:002016-06-29T04:46:49.016-07:00Hello Dear Jenny, I join with all of the others a...Hello Dear Jenny,

I join with all of the others and pray for comfort, as well as, recovery. You have always been a source of inspiration to me and a lot of my writing came easy with your encouragement. I, too, wish I could snap my fingers and have it all disappear. I pray for you, your family and your doctors. From the bottom of my heart, I wish only peace and happiness for you. You have touched so many with your story and your courage. God Bless you!noexcuseshttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-39830352557593553762016-06-27T19:13:26.151-07:002016-06-27T19:13:26.151-07:00I enjoy most of your articles,the articles are so ...I enjoy most of your articles,the articles are so nice for readers.

www.gofastek.comCindy Dyhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-15329251785542163982016-06-27T17:59:57.692-07:002016-06-27T17:59:57.692-07:00Came by just to see if you might have posted somet...Came by just to see if you might have posted something. I'm hoping and praying my friend.Bettyhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-11776549832665281182016-06-23T08:39:53.442-07:002016-06-23T08:39:53.442-07:00Thinking about you and wanted to say "Hi"...Thinking about you and wanted to say "Hi". Praying for you.
XXOOMy Journey Backhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-33850101319287532812016-06-22T09:17:50.827-07:002016-06-22T09:17:50.827-07:00Dear Miss Jenny... Thinking that I hadn't lin...Dear Miss Jenny... Thinking that I hadn't linked in awhile, I just came across this post. I am so sorry to hear about your health issues. You will be greatly missed in the blogging world. You brought many rays of sunshine into our lives. Thank you so much for giving of yourself to bring joy to all of us! Hugs and prayers sent your way from Arkansas!Esther Joyhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-74959406530680024652016-06-16T22:04:09.160-07:002016-06-16T22:04:09.160-07:00love and hugs and prayerslove and hugs and prayersSplendid Little Starshttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-31681872948495502372016-06-01T16:06:11.289-07:002016-06-01T16:06:11.289-07:00wishing you peace on your continued journey and ho...wishing you peace on your continued journey and hope the break gives you renewed strength.Gallery Juanahttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-71021998495419031322016-06-01T10:27:55.389-07:002016-06-01T10:27:55.389-07:00Take care. You have warmed my days with your wonde...Take care. You have warmed my days with your wonderful blog. I hope and pray you find relief from your pain. <3Bethhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-15082982835310117712016-06-01T08:31:59.325-07:002016-06-01T08:31:59.325-07:00Stopping in to Warm Your Heart today. Praying for...Stopping in to Warm Your Heart today. Praying for you. I hope your feeling better. Praying for Peace for you and Wisdom for your Doctors.
XXOOGrantham Lynnhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-17471261983445812462016-06-01T07:48:17.142-07:002016-06-01T07:48:17.142-07:00Just stopped by to let you know that I'm think...Just stopped by to let you know that I'm thinking of you. <3edenhillshttps://email@example.com:blogger.com,1999:blog-7688526164999265223.post-28860331539550723882016-05-30T19:00:31.551-07:002016-05-30T19:00:31.551-07:00Every person has the passion in any aspects or thi...
Every person has the passion in any aspects or things. If you love something and it came in front of you it completes your day and your mood turns into something you won't expected. I love your work and I want to read more about it. Visit my site if you have time . Thank you.

triciajoy.com

www.triciajoy.comandrea chiuhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-53590338825332031602016-05-26T09:16:31.251-07:002016-05-26T09:16:31.251-07:00Jenny, I pray you are feeling well right now. SO s...Jenny, I pray you are feeling well right now. SO sorry to hear that you are not well. I came for the Alpha-Thursday (I guess I haven't been around for a while). Praying for you.Maria Gaglianohttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-37348309627254551152016-05-24T18:06:34.642-07:002016-05-24T18:06:34.642-07:00I'm sorry that you aren't feeling better J...I'm sorry that you aren't feeling better Jenny. I hope to see you back here sooner, rather but later. Later is okay though. We've been following each other's blogs for a long time, and you are one of my favorite people out there. So you do what you have to do and please take care of yourself.
Until that time Jenny...Pat Tilletthttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-39149929388291657402016-05-22T19:33:00.615-07:002016-05-22T19:33:00.615-07:00Thank you for putting an effort to published this ...Thank you for putting an effort to published this article. You've done a great job! Good bless!

www.gofastek.comCindy Dyhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-77527923971253369422016-05-20T08:47:24.002-07:002016-05-20T08:47:24.002-07:00We will all miss you like crazy, Jenny, and I will...We will all miss you like crazy, Jenny, and I will hold you in my heart and prayers. You are one of the best blog pals I've ever made, and I hope you will stay in touch via email. Love you lots and am hoping against hope that things improve for you soon.

xo
Sue
=)Susan Andersonhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-55963142172631603512016-05-18T06:40:27.267-07:002016-05-18T06:40:27.267-07:00Just wanted to say "I'm thinking of you.&...Just wanted to say "I'm thinking of you." May your heart be warmed and filled with Love.
XXOOGrantham Lynnhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-63373335056228042312016-05-16T18:58:28.658-07:002016-05-16T18:58:28.658-07:00شركه كشف تسربات المياه بالرياض كشف تسربات المياه...
 شركه كشف تسربات المياه بالرياض
 كشف تسربات المياه بالرياض
 شركه عزل اسطح بالرياض
 شركه تسليك مجارى بالرياض
 شركة مكافحة حشرات بالرياض مؤسسه الحرمينhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-59805787359187395732016-05-15T16:37:00.031-07:002016-05-15T16:37:00.031-07:00Hi Jenny, Although I too stepped away from the bl...Hi Jenny,

Although I too stepped away from the blogging world for the last couple years, my thoughts of you and your amazing help in opening up a creative window in my life have never left. I am sorry that your health issues have taken such a toll over the years, so much so that you need to leave the blog world for now. I know how much you enjoy it. I hope your next pain management endeavor proves successful. Best wishes for you always.

Tom Goette
Sophisticated Lunacy

Tgoettehttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-48461093208061906132016-05-14T13:26:20.202-07:002016-05-14T13:26:20.202-07:00I am so sorry to read that you are in such pain, J...I am so sorry to read that you are in such pain, Jenny. You have supported so many people through their trials, and been such a source of positivity (is that a word? I'll use it anyway) I hope that the trials you're about to endure will prove helpful in the end and that we will hear from you now and again. Hugs to you.......Pondsidehttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-71258868923981883302016-05-14T08:40:05.727-07:002016-05-14T08:40:05.727-07:00Love you my sweet friend. I'm sending prayers,...Love you my sweet friend. I'm sending prayers, good mojo and positive vibes your way. Hoping you're able to manage the pain and enjoy your days. XOXOBusy Bee Suzhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-28531072640839028042016-05-13T20:07:54.956-07:002016-05-13T20:07:54.956-07:00Sorry to hear this. I know I haven't been arou...Sorry to hear this. I know I haven't been around much but I do think about you. Take careTo the Toy Box and Beyondhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-52376050193690877502016-05-13T20:07:43.128-07:002016-05-13T20:07:43.128-07:00Sorry to hear this. I know I haven't been arou...Sorry to hear this. I know I haven't been around much but I do think about you. Take careTo the Toy Box and Beyondhttp://email@example.com:blogger.com,1999:blog-7688526164999265223.post-3520122981586107842016-05-13T15:53:03.967-07:002016-05-13T15:53:03.967-07:00Oh, my friend! I am so sorry that you are in pain!...Oh, my friend! I am so sorry that you are in pain!! Maybe you should Vlog….you know…because the blogging world just won't be the same without you in it. I will pray for you! Stefhttp://firstname.lastname@example.com:blogger.com,1999:blog-7688526164999265223.post-48511481428824774502016-05-13T10:26:25.692-07:002016-05-13T10:26:25.692-07:00You will stay in my thoughts, and I wish you all t...You will stay in my thoughts, and I wish you all the best.Julie Kwiatkowski Schulerhttp://email@example.com


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Florida Cookery
The Royal Palm's restaurant, Florida Cookery, features regional Florida Fare with a focus on locally-sourced ingredients.
Recently recognized by The New York Times as "…a spot that really celebrates Florida cuisine in a laid-back atmosphere," our South Beach restaurant offers guests an approachable menu that is local, fresh and unpretentious in an outdoor setting that captures the energy of South Beach.
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text: Zipcar If you're looking for a recent IPO that looks poised to break out, then take a look at Zipcar ( ZIP), which operates a car-sharing network. Zipcar provides over 400,000 members, also known as Zipsters, with self-service vehicles that are located in reserved parking spaces throughout the neighborhoods where they live and work. This stock is off to a weak start in 2011, with shares off by around 12%. If you take a look at the chart for Zipcar, you'll see that this IPO has just started to break out above a key descending trend line that had been acting as resistance on the stock for the past couple of months. In fact, anytime the stock traded near this descending trend line, it failed and immediately trended lower. Now the stock has started to trade above the trend line and it looks ready to make a big run higher. What trades should watch for now is for a big upside volume to start moving into this stock. I would suggest watching for upside volume that's well above its three-month average volume of 646,000 shares as ZIP starts to challenge some of its overhead resistance prices. Related: 5 Heavily Shorted Market Leaders Set to Soar One could simply be a buyer of this stock on any weakness as long as ZIP stays above the descending trend line. I would add to any long position once you see ZIP take out some near-term overhead resistance at $25 to $26.37 a share. A move above those levels could set the stock up for a run back toward its all-time highs of $30.80 a share. It's worth noting that this stock is heavily shorted by the bears. The current short interest as a percentage of the float for ZIP stands at 16.1%. The short-sellers have also been increasing their bets from the last reporting period by 19.7%, or by about 656,000 shares. This stock could easily be setting up to short squeeze big, so keep this name on your trading radar. Prev 4 of 6 Next


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Discussion (2)
The need to be taking pics, just in case the car "disappears" again.
I'm a little more worried about Fred disappearing. Until (and unless) they find out who owned the car and who was driving it, he still doesn't have closure.
The book proofs for Volumes 5 and 6 arrived and are being reviewed in preparation for final printing and delivery on June 1st!
And some exciting news: Bill Strickland, Editor in Chief of Bicycling, has written a beautiful foreword for Volume 6. His piece is the perfect way to kick off our newest volume of Kickstand Comics!
Enjoy the ride,
Rick and Brian


text: Alumni & Giving / Meet Our Alumni / Class Notes print Your Class Network Ever wonder what's going on with your former classmates? Welcome to Class Notes online where you can stay abreast of the latest professional activities and other important milestones in your classmates' lives. Visit often, as we are frequently updating the site, with information compiled from alumni, including news published in local and national media. 2010s 2000s 1990s 1980s 1970s 1960s 1950s 1940s 1930s 2010 2011 2012 2013 2014 2015 Jenna M. Adams joined the New York City Department of Transportation as its Director of Legislative Affairs, where she is responsible for drafting legislation to enhance the City's transportation infrastructure, promote bicycle use, and make the streets safer, and for formulating strategies to advance the Agency's priorities in the City Council and the State Legislature. Adams previously served as Director of Intergovernmental Affairs at the New York City Landmarks Preservation Commission. (8/26/2015) Valerie Durollari Biberaj joined the Kate Spade & Company Foundation as the executive director and Kate Spade & Company as the director of philanthropic programming. She is responsible for grantmaking and programming promoting women's empowerment in underserved communities in NYC. She previously served as director of development at The Lunchbox Fund, a non-profit organization dedicated to providing a daily meal for orphaned and vulnerable school children in township and rural areas of South Africa. (9/9/2015) Michael Kwiatkowski joined Meyer, Suozzi, English & Klein as an associate with the bankruptcy and business reorganization department in the firm's Garden City, Long Island office. He was previously an associate at Hogan Lovells. (4/29/2016) Shai M. Mehani joined the legal department of Hudson's Bay Company as corporate counsel. Hudson's Bay Company is the parent company of Saks Fifth Avenue, Lord & Taylor, and Gilt. (7/18/2016) Jeremy Raphael is a senior attorney at Miller, Egan, Molter & Nelson LLP and leads the firm's New York office. He represents emerging technology companies and entrepreneurs. (3/9/2016) Related Links: Make a Gift Alumni Services BLS LawNotes News & Events We want to hear from you! Please send us your news by fax to (718) 625-5242 or complete our online form for publication on these pages and for the next issue of BLS LawNotes.


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The Picture Stack effect
The Picture Stack effect
Another new guided Edit option, that is new in Photoshop Elements 10, is the Picture Stack Effect. This effect creates the illusion of several pictures stacked on top of one another, that together makes one overall image. You have to see it to believe it. Let me show you how it works. I'm beginning this video in the Elements 10 Organizer, and I'm going to scroll down in my Media Browser. To find a file that I think will work well with this effect. And the image that I've chosen to use is this image here, called _MG_9967.JPG. So with this photo highlighted, I'm going to come over here to the Fix tab. Click on the Drop Down menu and choose Guided Photo Edit. When this image is open in the Photoshop Elements Editor, I'm going to scroll down to the category called Photo Play.
And I'm going to click on the Picture Stack Effect to make that option active. The first thing that's going to show up here is this preview of what the image looks like. And it ...
The Picture Stack effect provides you with in-depth training on Photography. Taught by Chad Chelius as part of the Getting Started with Photoshop Elements 10
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- Photoshop Elements
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- Chad Chelius
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In this issue: Five building studies of housing schemes in London by Peter Barber Architects, Edgley Design, OMA with Allies and Morrison and Hayhurst and Co. PLUS RIBA Stirling Prize shortlist revealed, what effect will Brexit have on housebuilding? and a profile of RIBA president Jane Duncan
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25 July, 2016 5:45 pm
Developer Capco has revised down the value of its major 7,500-home Earls Court scheme, designed by Farrells, after Brexit jitters
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Architecture students on the Centre for Alternative Technology's Part 2 course have built a floating pavilion on a reservoir within the school's site in Machynlleth, Wales
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Kenyan Legislators visit the US and Canada to learn about televising Parliament
SUNY/CID conducted a two-week study visit to the United States and Canada for the Parliament of Kenya. The visit was funded by the Kenyan National Assembly's Joint Committee on Parliamentary Broadcasting and Library with the objective of learning how proceedings and sessions are recorded and presented to the public in other legislatures.
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Making Livestock Production Profitable in Kenya's Arid and Semi-Arid Lands
Following the enactment of the 2010 Constitution and the introduction of a devolved system of governance, Kenya's counties have become centers for growth. One resulting opportunity is the development of the livestock sector, especially in those counties that fall in the Arid and Semi-Arid Lands (ASAL) counties.
County Workers in Kenya Sign Performance Contracts, Promise Better Service Delivery
The Tharaka Nithi County Government in Kenya signed performance contracts with its staff in a launch ceremony themed 'what is measured, gets done'. The ceremony was a culmination of introducing results based management, by SUNY/CID through the Agile and Harmonized Assistance for Devolved Institutions program (AHADI) project supported by USAID.
- SUNY/CID Welcomes a New Director
- Making Water Provision a Reality in Kenya's Arid and Semi-Arid lands
- Kenyan Legislators visit the US and Canada to learn about televising Parliament
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Robert Nakamura, et al.: "Stregthening Deliberative Bodies: Legislative Engagement Reference Paper" (pdf)
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At SUNY/CID we connect people and ideas for integrated development through the design and implementation of large, multi-year donor funded projects, institutional capacity building projects, and research-intensive assessments and evaluations. Learn more about us and our mission.
For additional information, feel free to peruse SUNY/CID's 25th Anniversary Publication.


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May 6, 2016 + WordPress Plugins
Top WordPress Plugins to Keep Your Blog Organized
Those of you who have dipped their toes in blogging probably know that it involves a lot of planning and organization. The ones who haven't started yet and are down the path of starting a blogging career have to keep that in mind. You should give a careful thought not only what you write about but also how you organize it and present to… Read More
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Photo Details
An afternoon of smiles and fun with close family. Seated front and center is June Evelyn Fisher Davidson holding Cheryl Diane Davidson, Left is Clifton Norman Hammett, Lavon Liebhart, Ida Hammett Liebhart, Clifton Maywood Hammett, Margaret Mary Pio Zeh Fisher Hammett, Mildred Eloida Palmer Pio Zeh, JoAnn Liebhart, Lee John Davidson.
at Picnic 1949, Ohio


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marketing director jobs near Industry, CA
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VP, Account Director
RAPP - Los Angeles, CA
Demonstrate a deep knowledge of marketing, communications and CMR/direct marketing principles. Marketing and agency experience a must....
Director Market Intelligence
Beckman Coulter, Inc. - Brea, CA
Director Market Intelligence. Organization to shape business and marketing initiatives. Bachelor's degree in marketing, business, or related field with....
Marketing and Sales Director
Regency Park Senior Living - Pasadena, CA
Senior assisted living organization seeks an experienced sales and marketing professional to lead community sales efforts and coordinate marketing activities to...
Sales and Marketing Director
Established Senior Living Organization - Pasadena, CA
Sales, marketing, assisted living, long term care:. Established Assisted Living organization seeks an experienced professional to lead community sales efforts...
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DTT - Los Angeles, CA
Marketing for technology. College Degree, preferably in Business, Marketing, or Communications. We are searching for a highly motivated, talented, and qualified...
Director of Marketing Initiatives
Mayfield Junior School - Pasadena, CA
The Director of Marketing Initiatives will be expected to:. The Director of Marketing Initiatives will lead the strategic communication and marketing...
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Society of Hispanic Professional Engineers - Industry, CA
Director and management on tactic approaches to relationship management and communications. The role of the Corporate Relations Coordinator is of paramount...
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Uniworld - Los Angeles, CA
10+ years of combined experience in PR and brand marketing (Online marketing or e-commerce experience is a must)....
Director of Marketing and Business Development
LEISURE GLEN POST ACUTE CARE CENTER - Glendale, CA
Leisure Glen Post Acute Care Center 330 Mission Road Glendale, CA 91205 P: 818-247-4476 *Leisure Glen Post Acute Care Center is a 125-bed
Sr. Director of Marketing
Trafalgar - Anaheim, CA
Director of Marketing. Director of Sales). Minimum 10+ years of marketing experience. Travel industry, Salesforce (or other CRM application) and digital...


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Many people were surprised to see that Teak Wood Thai and Sushi was coming to the large open space on 14th Street between N and Rhode Island Avenue. There are quite a few Thai restaurants within the Logan Circle neighborhood... especially with Thai Tanic located directly across the street. However, Teak Wood offers a classier and more upscale environment than some of the other local options, and also combines Thai food with a large Sushi bar to offer diners a nice mix of Asian cuisine.
The restaurant itself is much larger than it appears from the street. I was surprised to see how far back it goes, but also was pleased that they had dedicated a lot of space to a sushi bar towards the back that allows customers to enjoy a quick bite while watching their meal being handmade in front of their eyes. Teak Wood is filled with... a lot of teak wood. The tables and chairs are made of wood, and there are numerous large ornate mirrors carved from wood as well as buddhas and other interesting adornments throughout the space. It could be over the top, but it's actually quite okay because of the warm neutral colors and the relaxed vibe. While the restaurant is still in the midst of their "soft opening", it was nice to see a lot of locals testing it out as it has the potential to be a great neighborhood spot for those of us who live a few blocks away.
Teak Wood has two menus- their traditional Thai dishes which also offer many vegetarian options, and their sushi menu which has lots of choices including basic rolls for very reasonable prices as well as specialty rolls that can get a little more expensive depending on what you decide to order. I wanted to be sure that we got to try from both menus so my friend Andy and I started with a Spicy Tuna Roll as our appetizer. We both were holding our breath a little as we are in desperate need of a local spot with decent sushi as there aren't a ton of great options in the area. Thankfully the roll was delicious and the tuna was fresh and seasoned well with a nice kick to it. Good start to the meal.
I ordered the Pad Eggplant with chicken for my main course which included oriental eggplant in spicy black bean sauce with chicken. My friend ordered the red curry with chicken in coconut milk with eggplant, zucchini, yellow squash, and basil leaves. Both of us finished our plates completely and we were really impressed with the entrees. The eggplant in my dish was so soft and nicely cooked, and the sauce was just a touch spicy with a little sweetness to it at the end of each bite. The curry dish was aromatic and surprisingly light with a wonderful mix of vegetables in a pool of delicious sauce with hints of coconut and refreshing basil. I am anxious to try some of the seafood and beef options as well, but based on our positive first run of Teak Wood, I think I'll be heading back to round out my full taste test soon.
There were a few minor complaints that I had about Teak Wood including the discomfort of the chairs that are essentially wood slabs which will make it hard to enjoy a leisurely dinner, however, it is bound to do very well because of the Asian combination of cuisines and the light and airy space that it occupies. I think Teak Wood looks promising, and it might be one of the new post-gym dinner spots that I can add to the list along with Commissary and Sweetgreen. Let's see how the neighborhood responds, but I have high hopes.
Teak Wood
1323 14th Street NW
Washington, DC 20005


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Thanks for the reply.So, if I activate a shutter release, they will fire simultaneously?Thanks RoccoYour gonna miss 100% of the shots you never take, so keep shooting.Visit www.roccosphototavern.comThere's Something For Everybody At Rocco's Photo Tavern.Performance, Landscape, Portrait, Street, Creative and more.


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Fisher Space NonReflective Military Cap-O-Matic Space Pen, Matte Black (SM4B)
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The Cap-O-Matic feature makes it extremely easy to operate the pen, even with one hand.
I read the reviews before deciding to buy, and would like to address one of the consistent complaints against this pen's quality. Users reported on several occasions that the ball-point made clicking noises while writing. After a few days of use, I came upon the same problem, and found two ways to stop the clicking:
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2.) Second, because there are no gaskets inside the pen, there is nothing to prevent the cartridge and the inside of the casing from rubbing off on one another. This inevitably leads to wear ( especially if you press hard like me ), and the consequent 'clicking'.
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Needless to say, you will need to eventually replace the tape after a few months, but it really takes no time at all.
This is a fantastic pen for those who appreciate a simple design backed by excellent technology.
That's too bad, because the pen is very elegant looking and well balanced.
It is an excellent pen for outdoor activity - writes when wet, in any temperature, any orientation. I keep it in my hiking pack.
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Love fisher pens so much I own three. Looking to make that four.
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It's another wonderful product, but be warned this is a tiny pen! I wouldn't want to write on this sucker all day, else I would get annoyed. Read morePublished 1 month ago by Ray
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I've tried a few space pens, all of which were satisfactory. However, this one seems to be the cream of the crop. It's perfect if you are a pen-clicker like me. Read morePublished 2 months ago by J33f
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Use this pen everyday has not failed me yet just have to keep an eye out for whom i let borrow it because they want to keep it. Read morePublished 4 months ago by Javier and Andrea
Awesome pen. Clip hasn't broken yet, but seems somewhat flimsyPublished 4 months ago by Amazon Customer


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2004 Acura TSX 10100 miles
Recently my car can't accelerate at all. I rev it up till 3500 rpms but it takes so long for it to gain speed. And after I turn off the engine and get out, it smells like brunt rubber. What do I need to do?
Monday, December 28th, 2009 AT 10:59 AM


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Opening Nebraskas skies: Congress restores funds to North Platte airport
Davis at the Legislature: Paying tribute to the cowboy culture
More opinion
South Dakota Supreme Court: Cattle died from drowning in blizzard
Town Hall set to discuss mega-chicken plant near Fremont
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A traffic stop on Interstate 80 near North Platte Friday led to the seizure of 74 pounds of marijuana and two arrests.
A K-9 unit from the Dawson County sheriff's office indicated to the odor of drugs coming from the vehicle. A search of the vehicle led to the seizure of 74 pounds of marijuana that was located in an external luggage carrier on the top of the minivan.
The driver of the minivan, Jared J. Case, 36, of St. Petersburg, Fla., and a female passenger in the vehicle, Izula Maximillen, 30, of Arcata, Calif., were both taken to the Lincoln County Department of Corrections on charges of possession of marijuana with intent to deliver and child endangerment.
A two-year-old male child traveling with the couple was placed in protective custody with Health and Human Services.
Flatrock Publishing, Inc.
1300 E 4th St., Suite F
North Platte, NE 69101


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Reviewed By Matt P (FORT COLLINS, CO) Reviewed for a 2005 Audi Allroad —5/30/2016 9:24 PM
Easy to install. Sounds great with my bi turbo
Reviewed By Andrew K (Tonawanda, NY) Reviewed for a 2013 Audi A4 —7/4/2016 6:44 AM
Getting a lifetime oil less filter is a no brainier upgrade. Increased flow to the engine means a small increase in power and fuel economy. Which eventually pays for the filter in savings. Then instead of getting a new filter in 30k miles just wash the oil less one and you're good to go. Plus in most vehicles anyone can install the filter. It's incredibly simple and quick


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Tag Archives: video
We Gotta Have That Funk
Here's a riddle for you: What do Elton John, Kanye West, Skrillex, Frank Ocean, Wiz Khalifa and you have in … More »
Posted in News Tagged Bonnaroo, contest, Funkiest Dancer, Music Festival, video Leave a comment
Lauren Adams' Angels
When I find out there is a video on the internet featuring Jakob Karr that I somehow haven't seen, my … More »
A Love Letter to Ellen
We just couldn't let the year end without giving one final shout out to Ellen Degeneres. Basically, she wins 2013. … More »
Posted in News Tagged Ellen, Ellen DeGeneres, Halloween, So You Think You Can Dance, The Fox, Twitch, video 1 Comment


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Journey and Steve Miller Band Rock Tinley Park
* Additional fees apply. No coupon or promo codes necessary to enjoy the displayed discount price.
The last date listed for Journey and Steve Miller Band was Wednesday September 10, 2014 / 6:45pm.
Currently at Hollywood Casino Amphitheatre
Journey and The Doobie Brothers: Music Legends Live
- Full Price:
- $37.75 - $103.80
- Our Price:
- $20.00 - $55.00
Two of the most popular musical groups of the past 40 years, Journey and The Doobie Brothers, hit… More
Venue Details
Reviews & Ratings
Featured review from Goldstar Member
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I went with my husband and 13yo daughter. Our experience was made so awesome by the kindness of strangers. As we were waiting in line for the $10 upgrade, a gentleman gave us tickets for center stage, row W for free!! What an upgrade!!!
The music and performances were great!! So glad our daughter saw Journey in person.
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Both shows were great. It was too cold though to really enjoy it but that was my falt for not dressing accordingly. Missed most of Tower of Power.
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It was a fantastic show.
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Outstanding bands. LOVED Tower of Power
Food and Drink: First Midwest Bank Amphitheatre offers a wide variety of alcoholic beverages for purchase during your time at the venue. You are not permitted to bring any alcoholic beverages into the venue.
The First Midwest Bank Amphitheatre offers a wide variety of concession options. This includes cheeseburgers, hot dogs, nachos, pretzels, candy, popcorn, fountain soda (Coke Products), water, and Red Bull.
New items include the Portobello Mushroom Burger (vegetarian), a Tofu Rice Bowl created by Top Chef Hugh Acheson, Samba Hummus and Pretzels, and the new Braided Pretzel.
Drinking Water: Guests are permitted to bring in one factory sealed bottle of water up to one gallon in size for most events. Aluminum bottles, refillable plastic water bottles, and Camel baks are not permitted. This is subject to change without notice.
Water fountains are located near every restroom facility.