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Single-molecule techniques have been developed for commercial DNA sequencing1,2. One emerging strategy uses a nanopore to analyze DNA molecules as they are driven electrophoretically in single file order past a sensor3-5. However, uncontrolled DNA strand electrophoresis through nanopores is too fast for accurate base reads6. A proposed solution would employ processive enzymes to deliver DNA through the pore at a slower average rate7. Here, we describe forward and reverse ratcheting of DNA templates through the α–hemolysin (α-HL) nanopore controlled by wild-type phi29 DNA polymerase (phi29 DNAP). DNA strands were examined in single file order at one nucleotide spatial precision in real time. The registry error probability (either an insertion or deletion during one pass along a template strand) ranged from 10% to 24.5% absent optimization. This general strategy facilitates multiple reads of individual template strands and is transferrable to other nanopore devices for implementation of DNA sequence analysis.
A commercial nanopore device for de novo DNA sequencing will require integration of six features: i) Automated capture and processing of genomic DNA templates in single file order from a heterogeneous mixture over many hours. This is essential to eliminate extensive DNA sample preparation common to other sequencing technologies and thus fully exploit the speed of nanopore sequencing; ii) systematic spatial control at circa 5 Å precision; iii) temporal control at 0.1-1000 ms per nucleotide; iv) absence of complex active voltage control. This is necessary to avoid crosstalk in a compact electronic array of thousands of nanopores; v) a sensor that can determine single base identity; and vi) a counter that can identify transitions between nucleotides along homopolymeric regions.
Devices based on the pore-forming protein α-HL are common in nanopore technology 3-5, 8. Briefly, a single α-HL nanopore is inserted in a lipid bilayer that separates two wells that contain 100 μl each of a buffered KCl solution (Fig. 1a). Negatively charged single-stranded DNA (ssDNA) is added to the cis well. A voltage applied between the wells (trans side +) causes the ssDNA to enter and electrophorese through the nanopore. This event results in a brief current blockade that is influenced by DNA strand length3 and base composition5.
A consensus has emerged that the average rate of ssDNA electrophoresis through nanopores (approximately 3 μs nt-1 at 120 mV for α-HL) is too fast to allow accurate base identification 6. Therefore a functional nanopore sequencing device will require a means to systematically slow DNA template movement. One proposed strategy involves coupling a protein motor to the nanopore 7. This strategy is attractive because many processive enzymes including polymerases ratchet along DNA strands one nucleotide at a time, up to tens of thousands of times in succession in bulk phase 9. In addition, as the polymerase drives the DNA strand through the nanopore sensor, the force of the electric field acting in the opposite direction is predicted to hold the strand taut, and therefore reduce base read errors caused by Brownian motion10.
Thus far most progress has been made with DNA polymerases. T7 DNA polymerase (T7 DNAP) was shown to bind to DNA captured in the α-HL pore, and then catalyze nucleotide additions that advanced the template strand through the pore against an 80 mV applied voltage 11. Replication was blocked in bulk phase using synthetic DNA 'blocking oligomers', and was initiated only after each DNA template was captured by the nanopore with attendant removal of the block by voltage-driven unzipping. Although an important proof of concept, this method could not be used in a practical sequencing device for two reasons: 1) at most three sequential ionic current steps could be observed before the T7 DNAP dissociated from the DNA template under load; and 2) to remove the blocking oligomer and subsequently bind T7 DNAP, the DNA was tethered in the pore and driven back and forth by reversing voltage polarity at 10 ms intervals. This would result in an unacceptable level of crosstalk between pores in a compact commercial array.
More recent experiments showed that a B family polymerase, phi29 DNAP, remained bound to DNA captured in the α-HL pore approximately 10,000-fold longer than did A family polymerases12. DNA replication by phi29 DNAP controlled sequential movement of at least 50 bases through the α-HL pore from a precise starting point on a DNA primer strand without active voltage control. The rate of elongation and template displacement was tens of milliseconds per nucleotide, consistent with previously measured rates 13,14. However, these experiments relied upon transient chemical protection of the DNA primer terminus to prevent elongation and excision in bulk phase 12. This approach permitted only a 20 minute window to capture unmodified DNA from bulk phase, and therefore is not suitable for a commercial sequencing device.
In the work presented here we combine phi29 DNAP-dependent template control with an improved blocking oligomer strategy for capture, activation and electrophoresis of up to 500 DNA molecules in single file order through individual nanopores. Importantly, we discovered that blocking oligomers potentiated formation of phi29 DNA polymerase-DNA complexes in bulk phase that were quiescent for at least five hours. This eliminated the need for active voltage control to effect polymerase binding to DNA. It also enabled automated forward and reverse ratcheting of each DNA strand through the pore, exploiting phi29 DNAP in two distinct mechanistic modes for template strand analysis.
Figure 1b illustrates features of the blocking oligomer designed for use with phi29 DNAP. The DNA substrate is a 23-nucleotide (nt) primer annealed to a synthetic 70-nt DNA template (Supplementary Fig. 1). To protect the DNA primer from phi29 DNAP-dependent extension and digestion in bulk phase, a blocking oligomer (dashed line, Fig. 1b, i) is annealed immediately adjacent to the DNA primer/template (p/t) junction. The blocking oligomer includes a ~25-nt complement to the template strand and two acridine (z) residues attached to the 5′ end (Fig. 1b, ii). One of these acridines substitutes for a nucleotide and abuts the primer terminus; the other is an added 5′-overhang that is presumed to intercalate into the DNA duplex. The blocking oligomer was appended with a 3′- three carbon spacer (s) followed by seven abasic (1′, 2′-H) residues (x's). This appended segment has two functions: protection of the blocking oligomer against exonucleolysis by phi29 DNAP (Supplementary Fig. 2), and facilitation of blocking oligomer removal as the phi29 DNAP-DNA complex is pulled into the nanopore by an applied voltage.
The blocking oligomer protected the DNA primer strand from phi29 DNAP-dependent extension and digestion in bulk phase (Fig. 1c). In these experiments, DNA substrates and phi29 DNAP were incubated in nanopore buffer (0.3 M KCl, 10 mM Hepes/KOH pH 8, 1 mM EDTA, 1 mM DTT) supplemented with 10 mM Mg2+ for 5 hours at 23 °C. The products were then analyzed by denaturing polyacrylamide gel electrophoresis. Absent the blocking oligomer, phi29 DNAP digested the DNA primer strands (-dNTP, lane 3) or extended them (+dNTP, lane 4). In contrast, when protected by the blocking oligomer, the primer strands were neither digested (-dNTP, lanes 6), nor extended (+dNTP, lanes 7) by phi29 DNAP.
Our next objective was to remove the blocking oligomer from each individual DNA template captured in the nanopore so that phi29 DNAP could bind at the (p/t) junction. Initially we considered a proven strategy wherein active voltage control was used to unzip the blocking oligomer from the DNA template upon capture, followed by a voltage polarity reversal to drive the newly exposed DNA primer-template junction into the cis well to 'fish' for a polymerase molecule 11,15. This complex method proved to be unnecessary. Figure 2 shows an experiment using a 94mer DNA template strand bearing five abasic (1′, 2′-H) residues spanning positions +25 to +29 from the n=0 position (Fig. 2a). This abasic insert serves as an ionic current reporter during strand displacement through the α–HL pore 11-12,16-17. The DNA template was annealed to a 23-nt primer, and the 3′-terminus of the primer strand was protected from bulk phase modification by the blocking oligomer described above (Fig. 1b, ii).
Addition of this DNA construct alone to the nanopore cis chamber resulted in ionic current blockades with a median duration of ~4ms and an average residual current of 22.5 pA that are similar to translocation events for DNA substrates bearing short duplex regions described previously18. Events greater than 200 ms duration were rare. By comparison, Figure 2 shows an ionic current trace typical of 200 events when phi29 DNAP and dNTPs were subsequently added (see also Supplemental Videos 1 & 2). Capture of a DNA substrate molecule (Fig. 2b, i) resulted in a 23-24 pA residual ionic current that lasted several seconds (Fig. 2b, ii). Under a sustained 180 mV load, the ionic current then stepped through a series of discrete levels that traversed a 35 pA maximum (Fig. 2b, iii) before dropping to 22 pA and settling at a characteristic 25 pA amplitude (Fig. 2b, iv). These current levels were caused by sequential movement of the five abasic residues of the template strand through the α–HL trans-membrane pore. This effect is especially pronounced as the abasic residues enter then pass through the α–HL limiting aperture circumscribed by lys147 12. Upon reaching the 25 pA amplitude, the ionic current steps reversed direction and retraced the 35 pA peak (Fig. 2b, v) at about ten times the speed that the first peak was traversed, before stalling at 24 pA (Fig. 2b, vi). In this experiment, there were also 178 events where the ionic current series began as shown in Figure 2, but did not progress completely through the two peaks either due to enzyme dissociation (112/178), a stall within the first ionic current peak (23/178), or a stall in the second ionic current peak (44/178).
These nanopore data suggest that when phi29 DNAP was added to the nanopore bath in the presence of the protected DNA substrates, it formed stable but enzymatically inactive complexes due to the presence of the blocking oligomers. Activation of a given complex was achieved only upon nanopore capture. Successive stages of this hypothetical process are illustrated in Figure 2c: i) the open channel; ii) nanopore capture of a polymerase-DNA complex with a blocking oligomer bound; iii) mechanical unzipping of the blocking oligomer promoted by the applied voltage, which ratchets the DNA template through the nanopore. This gives rise to the first 35 pA current peak as the abasic insert traverses the major pore constriction; iv) release of the blocking oligomer, which exposes the 3′-OH terminus of the DNA primer within the polymerase active site; v) DNA replication by phi29 DNAP, which ratchets the template in the reverse direction through the nanopore, giving rise to the second 35 pA current peak; vi) stalling of DNA replication when the abasic residues of the template strand reach the catalytic site of phi29 DNAP.
This model makes three testable predictions. First, traversal of the first 35 pA peak due to voltage-driven unzipping of the blocking oligomer should be independent of phi29 DNAP catalytic capability. Therefore it should be observed in the absence of the Mg2+ ions required for both polymerase and exonuclease function. In experiments in which complexes of phi29 DNAP and the substrate shown in Figure 2a were captured absent free Mg2+, the first 35 pA peak was indeed traversed, followed by a stall at the 25 pA level, and eventual voltage-promoted dissociation of the complex (Supplementary Fig. 3). The second 35 pA peak was not observed in the absence of Mg2+, supporting the second prediction of the model: because traversal of the second 35 pA ionic current peak requires DNA replication, it should be dependent upon the presence of both Mg2+ and a full complement of dNTP substrates. As an additional test, traversal of this second peak should stall if one of the required dNTP substrates was withheld. Results consistent with this prediction are described in Supplementary Figure 3.
The third prediction of this model is that progression into the proposed replication-dependent peak should be influenced by the chemical identity of the DNA primer terminus. In particular, substitution of the 3′-OH terminus with a 3′-H terminus should delay appearance of the second 35 pA current peak by causing a stall as the p/t junction is positioned in the polymerase active site (iv in Fig 2b,c). This prediction also proved to be correct (Fig. 2d). That is, using a substrate bearing a 3′-H terminated primer, the first 35 pA peak was traversed as it was with the substrate bearing a 3′-OH terminus, due to voltage-driven unzipping of the blocking oligomer. The ionic current then stalled for several seconds at 25 pA (red horizontal arrow, Fig. 2d). Eventually, traversal of the second 35 pA peak was observed. This recovery was due to excision of the 3′-H terminated residue by the phi29 DNAP exonuclease and subsequent strand elongation beginning at the newly exposed 3′–OH of the neighboring dGMP nucleotide 12.
Together these experiments indicate that phi29 DNAP can be used to control forward and reverse ratcheting of individual DNA templates through the α-HL pore. In the forward direction, the template strand is driven 5′-to-3′ through the nanopore by applied voltage as its complementary blocking oligomer is unzipped at the bound phi29 DNAP enzyme. In the reverse direction, replication by phi29 DNAP extends the primer strand and thus biases movement of the template in the 3′-to-5′ direction relative to the pore. To quantify the average rate of DNA template movement per nucleotide in each mode, it was necessary to account for all 25 template nucleotides in each direction along the trace. We first established an ionic current map by building a composite derived from 10 traces that traversed both amplitude peaks (Fig. 3a,b). Thirty-two reproducible amplitudes were resolved for more than one-half of the replicated DNA templates using a 3 ms minimum cutoff. These amplitude steps were symmetric around a 25 pA midpoint (ionic current state 0) except for state -1 which was not observed in a majority of traces. To confirm that the 16 ionic current states in the replication-dependent peak correspond to displacement of 25 template nucleotides, we measured translocation pauses when one dNTP substrate at a time was reduced to 100 nM in the nanopore buffer while all other dNTPs were held at 100 μM (Supplementary Fig. 4). As anticipated, when these concentration-dependent pauses were assembled in logical order, 25 nucleotide additions to the DNA primer strand could be accounted for within ionic current states 0 to 16 of the map (n0 to n24, Fig. 3b). Along this 25 nucleotide DNA segment, the median rate of replication was 40 nt s-1 (IQR = 12 nt s-1, n=200). The ionic current states during voltage-driven unzipping (states 0 to -16) mirrored the states observed during replication. If we assume 25-nucleotide displacement during that process as well, the median rate of translocation during unzipping was measurably slower (median = 2.5 nt s-1 , IQR= 3.2 nt s-1, n=200).
To increase throughput we reduced the length of the blocking oligomer segment annealed to the target DNA template from 25 to 15 nucleotides (in the context of the blocking oligomer design shown in Figure 1b, ii). When annealed to a p/t substrate (Supplementary Fig. 5a), this blocking oligomer still afforded protection of DNA in bulk phase (Supplementary Fig. 5b) but allowed faster removal of the blocking oligomer at the nanopore. With this shorter blocking oligomer, up to 500 molecules were processed in single file order at a rate of 130 per hour through one pore.
We next determined the probability of template registry errors that occur when using this phi29 DNAP control strategy (Fig. 4a-d). There are two types: 1) errors that occur when the rate of strand displacement past the pore sensor exceeds the rate of data acquisition (the nucleotide at a given position would be missed or 'deleted' (compare Fig. 4a. i & ii)); and 2) errors that occur when the strand slips back and forth so that a given position is read more than once (a nucleotide would be falsely 'inserted' into the sequence order (compare Fig 4a i & iii)). Together these would result in registry-dependent "indel" errors - insertion or deletion of bases relative to the correct sequential series.
To accurately measure the frequency of these registry errors, it was essential to focus on ionic current states that correspond to single nucleotide positions bounded by clearly discernible neighboring states. The data in Figure 3b suggested that state 8 (bounded by states 7 and 9), and state 11 (bounded by states 10 and 12) within the replication-dependent peak satisfy these criteria. This was confirmed using a DNA mapping strategy described previously12-14 (Fig 4b & Supplementary Fig. 6). By extension, the mirror image ionic current states -8 and -11 of the voltage-driven peak were also used.
The model we used to quantify registry errors is illustrated in Figure 4d(i-iii) for the replication-driven ratchet with examples in Figure 4a (i-iii). A correct read was called when the current advanced from ionic current state 7 to state 8 and resided there for at least 3 ms before advancing to ionic current state 9 (Fig. 4d,i, green arrows; & Fig. 4a,i). A deletion error was called when the current advanced from ionic current state 7 directly to state 9 without residing in state 8 for at least 3 ms (Fig. 4d,ii, red dashed arrow; & Fig. 4a,ii). An insertion error was called when the current advanced from ionic current state 7 to state 8 and resided there for at least 3 ms, but then returned to state 7 for at least 3ms before advancing once again through state 8 and then to state 9 (Fig. 4d,iii , grey dashed arrow; & Fig. 4a,iii). Corresponding arrows centered at states -8 and -11 in Figure 4c (voltage-driven zipper) and state 11 in Figure 4d (replication-driven ratchet) have the same meaning.
Error estimates for individual molecules read once in each direction are summarized in Figure 4e. We found that the insertion error probability at one nucleotide spatial precision ranged from 5% to 10.5%, and the deletion error probability ranged from 5% to 15%. The combined error probability (either an insertion or deletion at a given position in a single pass) ranged from 10% to 24.5%. Errors at position 11/-11 were about ½ as frequent as errors at positions 8/-8.
We conclude that DNA substrates pre-bound to phi29 DNAP can be protected from enzymatic modification for many hours using blocking oligomers, and that activation of each complex for replication occurs upon capture on the α–HL pore. Because the DNA molecules are enzymatically modified only at the nanopore, it is possible to combine all components of the replication reaction (i.e. DNA template, dNTP, Mg2+, polymerase) in the nanopore chamber at time zero and run a lengthy analysis of many DNA templates without further user intervention. Thus, DNA strand sequence could be determined for an unknown template in a homogeneous preparation by analyzing a series of single molecules. Alternatively, DNA strand sequencing could be performed on molecules captured one-byone from a heterogeneous mixture (e.g. a genomic digest) with multiple reads per individual strand to achieve consensus.
Finally, processive circa 5 Å DNA template displacement was documented at two positions each for the voltage-driven zipper and for the replication-driven ratchet. This allowed us to make initial probability estimates for registry errors due to uncontrolled strand motion at the single nucleotide scale. These DNA template registry errors (10% to 24.5% combined probability for insertions and deletions at a given position) must be reduced for a commercial nanopore sequencing device. This will be a fruitful area of research because several factors that influence template motion and the ionic current signal-to-noise ratio have not been optimized. These include ionic strength, polymerase mechanics, enzyme identity, applied voltage, and composition of the trans-side buffer. Even absent improvements, proof of concept DNA sequencing is conceivable using this robust control strategy when coupled to nanopores capable of nucleotide discrimination18-20.
Heptameric α-HL was provided by Oxford Nanopore Technologies. Diphytanoylphosphatidylcholine (DPyPC) lipid was purchased from Avanti Polar Lipids. Wild-type phi29 DNAP (833,000 U ml-1; specific activity 83,000 U mg-1) was supplied by Enzymatics Corporation. Phosphoramidites (including abasic residue (dSpacer), 3′-spacer C3 CPG, and acridine) were from Glen Research. DNA oligonucleotides were synthesized at the Stanford University Protein and Nucleic Acid (PAN) Facility. Lyophilized oligonucleotides received from PAN were re-suspended in 7M urea, 0.1× TBE, purified by denaturing polyacrylamide gel electrophoresis, and quantified using a Nanodrop™ 1000 (Thermo Scientific).
2 μM each of a 70mer DNA template (Supplementary Fig. 1) and a 23-nt DNA primer bearing a 5′ fluorescein (6-FAM) label, were combined in the presence or absence of blocking oligomer (Fig 1b(ii)) at 2.4 μM final concentration. This mixture was incubated at 90 °C for three minutes in 1X TE , 100 mM KCl buffer, followed by slow-cooling to room temperature. 1 μM of preannealed DNA substrate was then incubated for 5 hours at room temperature (approximately 23°C) in nanopore buffer (22.5 μL final volume) supplemented with 10 mM MgCl2, 0.75 μM phi29 DNAP, and 100 μM dNTPs as indicated (Fig. 1). Reactions were terminated by the addition of buffer-saturated phenol. Following phenol/chloroform extraction and ethanol precipitation, dried DNA pellets were dissolved in 7M urea, 0.1X TBE and resolved by denaturing polyacrylamide gel electrophoresis. Gels were 17% acrylamide:bisacrylamide (19:1), 7 M urea, 1× TBE, and run at 18 W. 6-FAM labeled DNA primer products were visualized on a UVP Gel Documentation device using a Sybr Gold filter.
Setup of the nanopore device and insertion of an α-HL nanopore into a lipid bilayer have been described 4. Briefly, a single α-HL nanopore was inserted into a lipid bilayer that separates two wells that each contained 100 μl of nanopore buffer (pH 8). A 180 mV potential was applied across the bilayer and ionic current was measure through the nanopore between AgCl electrodes in series with an integrating patch clamp amplifier (Axopatch 200B, Molecular Devices) in voltage clamp mode. Data were recorded using an analog-to-digital converter (Digidata 1440A, Molecular Devices) at 100 kHz bandwidth in whole-cell configuration then filtered at 5 kHz using a analogue low-pass Bessel filter. Experiments were conducted at 23°C with 1 μM DNA substrate preannealed to blocking oligomer in nanopore buffer with 0.75 μM phi29 DNAP, 10 mM Mg2+, and 100 μM of each dNTP added to the nanopore cis well unless otherwise noted. A single nanopore experiment is defined as the time during which ionic current data were acquired from one α–HL nanopore in an intact bilayer before termination by bilayer rupture, loss of channel conductance, or completion of a preset number of translocation events. Successful forward and reverse DNA template translocations ranged for 50 to 500 per experiment .The ratio of fast (<200 ms) DNA alone events to polymerase-bound DNA events was approximately 10 to 1 unless otherwise noted.
Ionic current output from the nanopore device was analyzed using Clampfit 10.2 software (Axon Instruments) after additional smoothing of data at 2 kHz using a low-pass Gaussian filter unless otherwise noted. The standardized ionic current map in Figure 3b was compiled from ten translocation events from the experiment in Figure 2. These ten events each started at 23 pA, traversed two peaks at 35 pA, and ended by stalling at 23-24 pA. Ionic current steps within these ten translocation events were counted as discrete states if their durations were 3 ms or greater. For the data summarized in Supplementary Figure 4, sequence-dependent pauses were scored if the duration of a discrete ionic current state between 0 and 16 was 200 ms or greater, or if fluctuations between any two discrete states between 0 and 16 was 200 ms or greater.
The authors thank Oxford Nanopore Technologies (Oxford, U.K.) for supplying α-HL heptamers, Peter Walker and Yen Tran (Stanford University Protein and Nucleic Acid Facility) for expert oligonucleotide synthesis, Enzymatics Corporation for supplying concentrated phi29 DNAP, and Ai Mai for DNA purification. Hongyun Wang, Robin Abu-Shumays and Hugh Olsen commented on drafts of the manuscript. This work was supported by NHGRI grant HG006321.
1Nanopore Group, MS SOE2, Department of Biomolecular Engineering University of California, Santa Cruz, CA 95064.
GC co-wrote the manuscript and performed and conceived experiments, KL conceived experiments and edited the final draft, HR designed and performed PAGE assays, CL performed nanopore experiments, KK articulated the indel error problem in the context of nanopore sequence analysis and co-wrote the paper, and MA co-wrote the manuscript and directed the project.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Jobless benefits end for nearly 15,700 in Conn.
The recession in Connecticut is lasting long enough to generate a grim new statistic: unemployment benefits expired this year for 15,687 jobless residents, a number that swells each week by an estimated 500 people.
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House of Run and Tri
3709 Eastern Ave, Cincinnati, OH 45227 513-871-2200
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Hilton Garden Inn Troy, Troy
- Free WiFi and free parking
Main amenities
- 127 smoke-free guestrooms
- 2 restaurants
- Indoor pool
- Breakfast available
- Free airport shuttle
- Fitness center
- 24-hour business center
- Coffee/tea in a common area
- Air conditioning
- Daily housekeeping
- Garden
- Lobby fireplace
What's around
- Rensselaer Polytechnic Institute (1.1 mi / 1.8 km)
- Experimental Media and Performing Arts Center (1.2 mi / 2 km)
- Arts Center of the Capital Region (1.4 mi / 2.2 km)
- Troy Savings Bank Music Hall (1.5 mi / 2.3 km)
- Rensselaer County Historical Society and Museum (1.5 mi / 2.4 km)
Hilton Garden Inn Troy, Troy
Hotel Info: 855-239-9477from $118
- One King Bed, Accessible
- Presidential Suite, 1 King Bed
- One king bed evolution room
- Junior Suite, 1 King Bed
- Room, 2 Queen Beds, Accessible
- Two queen beds evolution room
- Room, 1 King Bed, Accessible
- Access one king evolution room
- Evolution suite one king bedroom
- Room, 2 Queen Beds, Accessible
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 127 rooms
- This hotel is arranged over 8 floors
- Check-in time starts at 4 PM
- Check-out time is noon
- Express check-out
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
- Minimum check-in age is 21
Travelling with others
- Pets not allowed (service dogs only)
- Free WiFi and wired Internet access in public areas
- Free WiFi and wired Internet access in rooms
- Free airport shuttle during limited hours *
- Free train station pickup
- Free self parking
Other information
- Smoke-free property
Nearby Three Star Troy hotels
In the hotel
- Full breakfast daily (surcharge)
- 2 restaurants
- Bar/lounge
- Room service (during limited hours)
- Coffee/tea in a common area
- Indoor pool
- Fitness facilities
- Golf lessons available nearby
- Golfing nearby
- 24-hour business center
- Number of meeting rooms - 12
- Conference space
- Meeting rooms
- Conference space size (feet) - 32,000
- Conference space size (meters) - 3
- Dry cleaning/laundry service
- Laundry facilities
- Free newspapers in lobby
- Wedding services
- Number of buildings/towers - 1
- Year Built 2009
- Elevator/lift
- ATM/banking
- Safe-deposit box at front desk
- Garden
- Fireplace in lobby
- Television in common areas
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- In-room climate control (air conditioning)
- Air conditioning
- Minibar
- Coffee/tea maker
- Iron/ironing board
- Blackout drapes/curtains
- Sleep Number by Select Comfort mattress
- Private bathroom
- Shower/tub combination
- Free toiletries
- Hair dryer
- 32-inch LCD TV
- Pay movies
- Premium TV channels
- IPod docking station
- Desk
- Free weekday newspaper
- Free WiFi
- Phone
- Refrigerator
- Microwave
- Daily housekeeping
- Connecting/adjoining rooms available
Top Troy hotels
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"We only stayed one night, but our room was very comfortable. We did not get a chance to explore the property as much as we wanted, but were very impressed with what we saw.…"Jul 26, 2016
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DiningRecovery Sports Grill - This restaurant specializes in American cuisine and serves lunch and dinner. Guests can enjoy drinks at the bar.
Great American Grill - This restaurant specializes in American cuisine and serves breakfast only.
Hilton Garden Inn Troy, Troy's small print
Also known as
- Hilton Garden Inn Hotel Troy
- Hilton Garden Inn Troy
- Troy Hilton Garden Inn
- Hilton Garden Inn Troy Hotel Troy
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
For transfers, guests must contact the property before arrival at the number on the booking confirmation.
Optional extras
Rollaway beds are available for USD 15 per night
Full breakfast is offered for an extra charge of USD 11.95 per person (approximately)
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Recent reviews
Excellent 4.4 /5 from 189 reviews
We stayed here while my son went to camp at RPI (it was cheaper than him staying on campus, in a double w/o air conditioning in the middle of July for a week). The hotel was very close to RPI and it was very accommodating. My younger son and I entertained ourselves during the day by going to Albany which was about a 15-20 minute drive and Saratoga was about a 30 minute drive from the hotel. There isn't very much to see while in Troy.
Nice hotel. Good breakfast cheap!
Nice hotel. Not walkable to nightlife down by the river.
attending a wedding near by
Very pleasant staff. Clean, comfortable rooms. Good buffet breakfast. We will be back
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Vancouver, BCCanada
October 18, 2012
I am looking to write a book within the next 12 months.
Hello. My name is Sam. I am currently in the midst of a career change from real estate to becoming an author.
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Campus NewsRowan hosts Attracting Women into Engineering for middle school girls | More
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American Opportunity & Lifetime Learning Tax Credit Info
The American Opportunity Credit takes the place of the HOPE Credit. The HOPE credit is for tax years 2008 and prior. The Lifetime Learning Tax Credit remains.
IRS Publication 970, Tax Benefits for Higher Education will assist taxpayers in determining their eligibility for the tax credits. You may obtain this Publication from any IRS office, or download it from the Internet at www.irs.gov/forms_pubs/pubs/p970toc.htm. The IRS addresses specific questions that taxpayers may have concerning their eligibility for the tax credits at http://www.irs.gov/newsroom/article/0,,id=213044,00.html.
In addition, if you have not supplied your social security number to Rowan University, please complete the IRS FORM W-9S part 1, which can be found at http://www.irs.gov/pub/irs-pdf/fw9s.pdf.
Form 1098T will be mailed to students by Jan. 31st, unless the student has opted to receive this form electronically. It is informational only. It alerts the student that they may be eligible for federal income tax education credits.
The college provides in Box 2 the tuition and fees that were billed to you and qualify as tuition/fee expenses. IRS education credits are based on amounts paid by you, not billed to you. To determine the amount of qualified tuition and fees paid, and the amount of scholarships and grants received, a taxpayer should use their own financial records.
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Thanks for the feedback fellow wardens! :)
Type: Posts; User: avilla; Keyword(s):
Thanks for the feedback fellow wardens! :)
I'm new to tanking, although I think I have a fairly good grasp of the warden's tanking skills. As I understand it, the only skill that enables us to grab multiple mobs fast is Defiant Challenge (10...
I have to agree with you there. Time for an update.
Greetings! You might like to consider the Celainedhil (http://www.celainedhil.com/index.php). We're a small and close-knit group, we've been around a long time, and we're still going strong with a...
Suilad randir!
The Celainedhil (the Elves of the Two Rivers) still flies its banner. Please feel free to contact me or Icidian in game, and see our website at http://www.celainedhil.com/index.php....
We are still recruiting intrepid adventurers of all races and classes.
Come explore Dunland with us!
We are holding our Summer Recruiting Drive! If you'd like to learn more about us, please contact me or see us at the Party Tree, Thursday, 6/30, at 8EST.
Does anyone know of a good newbie guide for the Lost Temple, particularly T2?
I see that Colred wrote a nice one for Sari-Surma:...
Welcome! You'll find all kinds of players here, from serious raiders to RPers.
The Celainedhil has always encouraged RP, and we have several new RP events coming up this summer. Most of our members are in the US, but one of our best RPers is a fellow Aussie.
Check us out at...
We are still recruiting stalwart adventurers of all classes, races, and levels.
Whether you're looking for regular instance runs, roleplaying events, or easy-going comeraderie, you will never...
Elven tradition. An easy-going atmosphere. Mutual respect, loyalty, and support. What more could you ask?
Consider making us your home in Middle Earth.
It is worth it. Once you reach L57 or so, break out into the light to visit Lorien and then Mirkwood. Once you reach L60, Enedwaith awaits you; it's beautiful and filled with interesting quests. ...
Indeed we will. Good to hear from you Keptwise.
We missed it too, but what a simple and wonderful idea. Please do it again.
What a great idea. I'll spread the word to the Celainedhil.
Many of our members continue to enjoy storytelling and RP. Come see us!
People of Middle Earth, the Celainedhil are still recruiting.
If you're a mature gamer looking for a strong, friendly, and tight-knit kinship, please consider joining us.
Please Turbine. If Codemasters can do it, so can you. Make it happen. We may lose a highly respected member of our kin who was recently hacked. This will severely weaken us, and if it...
The Celainedhil are still seeking good people.
Please check us out--we could be the home for you.
Then when Fingon heard afar the great trumpet of Turgon his brother, the shadow passed...
Mae govannen!
Welcome to the Celainedhil, the Elves of the Two Rivers.
For intrepid new warriors and heroic veterans, I encourage you all to consider the Celainedhil kinship for your new...
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text: AdChoices Terms of Use Privacy Policy Hide TOP 10 Top 10 Fitness Top 10: Push-Up Pro Variations Share Tweet 0 Shares 10. Weight vest It sounds simple, but donning a 5-or-10-pound weight vest while you do traditional push-ups will add an immediate challenge and help you build muscle just a little quicker. As this becomes easier, it's fun to wear a heavier vest and see how much weight you can carry. If you don't want to spring for a vest, just add any sort of weight to your back or have a workout partner push down on your back as you rise. Next Page TOP 10 9 8 7 6 5 4 3 2 1 Comments Share your opinion Your name More Like This Fitness & Workouts 7 Exercises That Will Quickly Add Muscle To Your Frame Read More Fitness & Workouts This Workout Plan Will Help You Gain 10 Pounds Of Muscle In Just 12 Weeks Read More Fitness & Workouts How One Actor Got Ripped For Elysium In 3 Months Read More Fitness & Workouts These Progress Pics Are Proof That Anyone Can Bulk Up Read More Fitness & Workouts We're About To Make It Super Easy For You To Achieve The Perfect Deadlift Read More Sponsored Ad Content Fact: You Aren't As Cut As You Could Be Read More Around the Web Special Features Special Feature The Ultimate Guide To Hooking Up Online Read More Guy Gear The Best Watches Under $500 Read More


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Home Prices Fell 3.9% in January From Prior Year, FHFA Says
U.S. home prices fell 3.9 percent in January from a year earlier as the housing market struggled to recover from the worst crash in seven decades, according to the Federal Housing Finance Agency.
The drop was led by an 8.6 percent slump in the region that includes Arizona and Nevada, followed by a 5.6 percent retreat in the area that includes Florida, the agency said in a report today. Prices nationwide fell 0.3 percent from December, compared with the 0.2 percent decline that was the average estimate of 17 economists in a Bloomberg survey.
Mounting foreclosures are depressing values and bloating the inventory of properties for sale. The number of previously owned homes on the market rose 3.5 percent in February to 3.49 million, the biggest gain in almost a year, the National Association of Realtors said yesterday.
"Foreclosures remain extraordinarily high, which is adding to the supply of homes and depressing prices," said Richard DeKaser, an economist at Parthenon Group in Boston.
The number of homes in foreclosure in January rose to a record 2.2 million, according to Lender Processing Services Inc. in Jacksonville, Florida. About 23 percent of homeowners with mortgages had negative equity in the fourth quarter, meaning their home-loan balances were higher than the value of their properties, CoreLogic Inc., a research company in Santa Ana, California, said in a March 8 report.
Fannie, Freddie Loans
The FHFA measures transactions of homes financed with mortgages backed by Fannie Mae or Freddie Mac. The data are based on repeat sales transactions that compare prices of the same properties over time. The report measures changes in values without providing a specific sales price.
The median U.S. price for a previously owned home sold in January was $157,900, according to the National Association of Realtors. It fell last month to $156,100, a nine-year low, the Chicago-based group said yesterday.
Sales of U.S. previously owned homes dropped 9.6 percent in February to a 4.88 million annual rate, according to the National Association of Realtors' report. Purchases fell in all regions, led by a 12 percent decline in the Midwest and a 10 percent slump in the South.
At the current sales pace, the inventory of homes on the market in February would take 8.6 months to sell compared with 7.5 at the end of the prior month, according to the Realtors group. About 39 percent of the sales were properties with mortgages in default.


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Calgary, ABCanada
June 3, 2013
I was just introduced to SFQ at a workshop I attended with Sue Crites (in Edmonton) and I am interested in learning more!
All aspects!
Moved to Calgary recently from Edmonton and I am interested in exploring anything to do with alternative health and wellness.
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Category Archives: evangelization
Misericordiae Vultus 20abc: Mercy and Justice
We turn to the topic much fretted about these days. 20. It would not be out of place at this point to recall the relationship between justice and mercy. These are not two contradictory realities, but two dimensions of a … Continue reading
Misericordiae Vultus 19: Conversion
Pope Francis has not let up on his challenge to organized crime and other forms of political corruption. Another mention here: 19. May the message of mercy reach everyone, and may no one be indifferent to the call to experience mercy. … Continue reading
Misericordiae Vultus 18: Missionaries of Mercy
Pope Francis explains Missionaries of Mercy. As I read this, I have a friend I'd like to nominate. 18. During Lent of this Holy Year, I intend to send out Missionaries of Mercy. They will be a sign of the Church's … Continue reading
Misericordiae Vultus 17cd: Confession
Some words on one of Pope Francis' favorite sacraments, an initiative we've seen in many dioceses around the world: The initiative of "24 Hours for the Lord," to be celebrated on the Friday and Saturday preceding the Fourth Week of … Continue reading
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Misericordiae Vultus 17ab: Lent in a Jubilee of Mercy
Lent 2016 will be a special time for the Church. Remember, we will be in year C, perhaps the cycle most dedicated to the theme of penance and mercy as it is proclaimed in the Lenten Lectionary. 17. The season … Continue reading
Misericordiae Vultus 16: The Isaian Mission of Jesus
One of the key Gospel passages is the commencement of the Lord's public ministry. In reading the scroll at synagogue, Jesus explains the vector of his service for and call to Israel. And to us, certainly. 16. In the Gospel … Continue reading
Misericordiae Vultus 15c: Inescapable Mercy
Yesterday we were reminded of the works of mercy. We cannot escape them, it seems. Pope Francis offers some concrete examples in conducting these in the jubilee and beyond: We cannot escape the Lord's words to us, and they will … Continue reading


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MAYS LANDING - A defendant in an upcoming voter fraud trial has been accused of coaching a witness on how to testify.
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Computer problem
2001 Cadillac DTS V8 Two Wheel Drive Automatic 61550 miles
Car keeps cutting off while driving, however, will start right back up again. Now the Check engine light is on and had a diagnostics test done said Crank Shaft Sensor (upper and lower) are gone bad. Is this a common problem with this model Cadallic?
Wednesday, February 11th, 2009 AT 7:15 AM


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Existing Posts
But then I wouldnt be able to view all the useless geekiness on this site...
Go ahead
Id rather stick pins in me eyes.... How dull.....
check out my Maude DVD website: www.maudeondvd.com
At long last : MAUDE - The Complete First Season While you guys fight it out over Bea Arthur, I'm going for Adrienne "Boom Booom" Barbeau
Well then your being FAR too serious.......
Yes, when you're dealing with a serious individual.
Oh okay!! But I meant comedy? Or such like.. Yes I like to play with words.. Is that bad??
a theatrical play.. what else do they perform on Broadway?
you remind me of someone who is always playing with words..
you remind me of someone who is always playing with words..
Performs in what context??
She's a Broadway actress.. she performs.
Perfom what?? Tricks???
Seems you haven't heard her perform either
She looks like a bloke
Then she served her purpose
She makes my body do strange things
you gotta love the artwork!
Just so that studios can give a title maximum publicity at a given time I imagine. It's a little bit annoying but we all do our best to try and adhere to their rules - apart from that particular site.
Why do these rules exist though?
(just curious)
(just curious)
Would be very nice if that site would stop breaking announcement dates. Shouldn't have been added until today (8th January). That's the reason neither us or TVShowsOnDVD.com added it.
Can't get enough of that fox Bea Arthur!
At long last : MAUDE - The Complete First Season
great eh ?
great eh ?
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28,981,249,043 zombies slaughtered...
Valve has announced that a total of 28,981,249,043 zombies have been bludgeoned, hacked apart, perforated or exploded since Left 4 Dead 2 was released on 17 November 2009. That's nearly 29 billion in 86 days. Not bad.
The games developer offers a few statistics to compare that number to. The entire population of the planet has been killed 4.26 times over, for starters. It also means that we're about quarter of the way through killing the total number of humans who have ever lived - about 100 billion. If you say a zombie's average height is 6 feet, then you could stack them end to end around the world 1322 times.
We love that Valve implemented a total zombie kill counter in the game in the first place, and hope that the company has a big screen in its HQ that constantly ticks upward at a rate of about 4000 per second. Valve doesn't specify what the total is when you add the figures from the original Left 4 Dead - who knows what heights we've scaled there.
Still, it's a mightily impressive figure. If you've contributed, then give yourself a pat on the back.
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|Don't want to.
#4955956, By Sub-kamikaze Left 4 Dead Organise Online Games Thread.
Sub-kamikaze 2,417 posts
Seen 6 years ago
Registered 9 years ago
Pokémon Go guide, best tips and tricks Best ways to catch and level up Pokémon out in the real world.
How to get XP fast in Pokémon Go Earn a whopping 60,000 XP in 30 minutes.
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|Don't want to.


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Citizens Bank recently provided a $7.2 million construction loan to finance the rehabilitation of a historic mill complex in Providence, which is slated to become a culinary and startup incubator. more
By PBN Staff
A New Zealand-based custom home builder, G.J. Gardner Homes, has launched a New England franchise with headquarters in Dracut, Mass., and is seeking contractors, entrepreneurs and suppliers. more
By Mary MacDonald
Legislation that could allow more foreclosed homeowners to stay in their homes, and repurchase them through nonprofit organizations, has been approved by the General Assembly. more
By Mary MacDonald
The state's housing market rebounded in 2015 to its largest volume of single-family sales in a decade, according to statistics released Wednesday by the Rhode Island Association of Realtors. more
By Mary MacDonald
The Massachusetts economy is continuing its "robust expansion" as employment is growing at rates not seen since the late 1990s, according to the MassBenchmarks editorial board. more
By Lori Stabile
Melina Lodge has been appointed as the executive director of the Housing Network of Rhode Island, an association that represents 16 nonprofit community development corporations. more
By Mary MacDonald
Rhode Island Housing has approved refinancing or tax credit packages for three projects that will rehabilitate housing developments in several communities. more
By Mary MacDonald
In 2015 the national branch of the Local Initiatives Support Corporation invested $1.3 billion in grants, loans and equity to projects, $315 million of which was invested into local Rhode Island housing projects, according to the organization's annual report released earlier this month. more
By Emily Gowdey-Backus
Rhode Island will receive $3 million in federal funds for lead-hazard reduction programs, as well as $400,000 in Healthy Homes supplemental funds, U.S. Sen. Jack F. Reed announced on Monday. more
By PBN Staff
Washington Trust recently provided $12.5 million to refinance an off-campus student housing complex near the University of Connecticut. more
By PBN Staff


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3 ways to cut your capital gains taxes
Cutting your taxes through capital gains is a very smart financial planning strategy.
Did you know that Canadian capital gains taxes are actually taxed at a much lower rate than interest? This is a huge advantage to investors and can substantially… Read More


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I live in house with two other people, in the heart of Uptown and I'm loving every minute of it! I enjoy biking the lakes, seeing live shows, walking to my favorite bars and cafes, checking out new restaurants, and otherwise having a good time.
I'm all about pursuing my goals and dreams, and I definitely know who I am and what I'm looking for out of life. I love it when I meet others who are also on that level, people who know what they want and are more than willing to go after it!
Yeah, with the wild hair and everything, hahaha.
I do professional branding and design, some of which I do for the government, but that work also entails some marketing, copywriting, event planning, and project management.
Someday, I'd like to release a few tracks to get my singing talent out there, and meet some other people to work with on writing original material. Music is hands down my passion in life.
Movies: Drop Dead Fred, Donnie Darko, Land Before Time (haha), The Goonies, Fifth Element. Aeon Flux.
Shows: Battlestar Galactica, Arrested Development, Breaking Bad, Bob's Burgers, Trailer Park Boys, LOST, The IT Crowd, The Walking Dead, Stargate Atlantis, Fringe. Pretty much anything hilarious, intellectual or sci-fi.
2) Positivity
3) Positivity
4) Positivity
5) Positivity
6) Negativity
5:1 ratio, preferred.
D/s dynamics
(imagination is key)
- A grown adult; reliable, considerate, kind, caring, supportive, emotionally mature and available; accountable.
- Has a career, or is actively working on one's goals; financially stable/responsible.
- Doesn't call women "girls."
- Is fulfilled in life. (happy, self-assured, "knows thyself," has a lot of interests, has close friends, is accepting and respectful).
- We have shared values and a similar idea of the future (conservatives need not apply).
- Has had good romantic relationships, which ended amicably.
- Still knows how to have fun and be positive; willing to play just cuz we can!
- Must love dogs.
Awesome! High fives. - Now get to messaging!!


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The Mix-Up
|CDN$ 16.75 & FREE Shipping on orders over CDN$ 25. Details
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Track Listings
|1. B For My Name
|2. 14th St. Break
|3. Suco De Tangerina
|4. The Gala Event
|5. Electric Worm
|6. Freaky Hijiki
|7. Off The Grid
|8. The Rat Cage
|9. The Melee
|10. Dramastically Different
|11. The Cousin Of Death
|12. The Kangaroo Rat
Product Description
Product Description
The Mix-Up is Beastie Boys' first-ever full album of all-new instrumental material. The follow-up to 2004's To The 5 Boroughs, The Mix-Up features Diamond, Horovitz and Yauch back on drums, guitar and bass, with able assistance from Keyboard Money Mark and percussionist Alfredo Ortiz, on 12 brand new wordless, sample-less, scratchless originals. Sure to please fans of the instrumental cuts from Check Your Head and Ill Communication and the cult hit compilation album made up largely of those tracks, The In Sound From Way Out!, The Mix-Up finds NYC's favorite sons drawing on one of their arsenal's primary strengths and pushing it into bold new directions.
Having long since shed their image as hip-hop's clown princes, the Beastie Boys now bring what feels like their emeritus recording, a celebratory instrumental memoir of all of the influences (except punk) that brought them to their secure place among hip-hop's fickle elite. The party opens with the aptly titled "B for My Name," its plodding bounce staking claim to the mid-tempo path the album treads almost throughout. "14th St. Break" picks up the pace, especially in the auxiliary percussion breakdown, complete with rally whistle. Then, beginning with "Suco de Tangerina," the album drops into a deep groove cut from dub- and dancehall-tinged ostinati that carry through a full third of its tracks. Among these, "The Gala Event" suffers from a lack of developmental motion that characterizes many of these tracks, but highlights still abound. "Off the Grid," for example, departs from the otherwise unbreakable chill and rips the proceedings wide open, blooming again and again in a series of pulsing riffs that celebrate the very institution of the instrumental groove. More than 20 years since Licensed to Ill took a long, irreverent piss into the mainstream, it seems you can still fight for your right to party. --Jason Kirk
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Top Customer Reviews
While 'The In sound from Way Out' was a compilation of instrumental songs from other albums like 'Ill Communication', 'Mix-Up' is a collection of original instrumental songs created for the actual album.
Songs like Suco de Tangerina and Biscuits and Butter that are grittier than the sounds coming from bands such as Soulive and more raw that Medeski Martin and Wood. It's unique but easy to listen to. I really like it.
Most Helpful Customer Reviews on Amazon.com (beta)
Maybe the Beasties weren't trying to sound like Medeski, Martin & Wood. Maybe they were just doing their own thing. Expanding on a sound and style they had already established if only hinted at on previous albums. And maybe you could write better music yourself right chief? I highly doubt it.
This is a great album and a natural progression from a band that has long since earned the right to do whatever they want to do. I hope this becomes a trend and we see more instrumental releases from them.
1. You like the BB's as hip hop artists, especially the funny lyrics, and enjoy the old school beats. You like Hello Nasty and To the Five Boroughs more than Check Your Head. Chances are good that you won't care for this album---don't buy it.
2. Your favorite album is Check Your Head and you really like the funky instrumental interludes on that and other albums of that era. BUT, you are not an avid listener of funk/acid jazz and are not familiar with Medeski, Martin, and Wood, etc. You will probably like this album and should buy it.
3. You fit into Category 2 EXCEPT that you ARE familiar with MMW, recent collaborations by John Scofield, Grey Boy Allstars, etc. You will be disappointed in this album because it just doesn't measure up. Frankly, this album comes off as very amateurish and shows that, though the BB's have developed some chops, they can't pull off a full album of instrumentals. The interludes in Check Your Head were just that---interludes---and you can't support a full album on interlude music. Not being a hater---I love the BB's too---but check out any of Stanton Moore's albums and you will see exactly what I mean.
You can hear how much fun the guys had recording this. It's a funky, fresh release. It's great chill out music. Buy "The Mix-Up" now; you won't regret it. Plus, you'll enjoy and appreciate it long before the Johnny-come-latelys come around and start singing its praises!
For those of you out there who want something a little groovy and different, get it. Meanwhile I'm going to mix myself a gimlet and listen to a great little combo called the Beastie Boys. Their new album is cool as hell.
This is the perfect time for them to stop talking and showcase their musical talents - without sampling. These guys love to jam out, and it shows.
I've been a huge Beastie Boys fan since 1985, and I do enjoy The Mix Up. I'm giving it 4 stars only because many of the songs tend to blend together, almost as if the album is one continuous track. There's not quite as much grit in this album as I had expected when looking back at the prior instrumentals.
It is very enjoyable to listen to, and I applaud the Beastie Boys' move away from rapping on their albums. Just buy it - it's great for chillin.
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text:
Maritime Today
The Maritime Industry's original and most viewed E-News Service
In the next few years, demand for bandwidth on the high seas will grow, in no small part due to technology that is making operations more efficient and keeping crews and passengers healthy,
Bored with palm-fringed beaches and turquoise seas? Then the gigantic oil platforms of the North Sea beckon. The first ever "rig-spotting" cruise just ended off the coast of Norway,
Three State-owned enterprises from the shipping, tourism and construction sectors will cooperate to equip up to eight cruise liners and offer diversified sightseeing
The Panama Canal authority (ACP) says a Chinese container ship's damaging scrape with the canal's new wider locks was caused by bad weather, Reuter quotes ACP's administrator, Jorge Quijano.
The Fishing Company of Alaska, based in Renton, owns the 238-foot Alaska Juris that started sinking in the Bering Sea shortly before noon on Tuesday, says a report in Seattle Times.
A barge sank east of the Galveston Causeway railroad bridge Tuesday, causing the U.S. Coast Guard to issue a safety zone and temporarily restrict traffic on the Gulf Intracoastal Waterway.


text:
Hey guys,
I've been struggling with this for a while now. On my site I have a login section that is opened from a ThickBox. The design went slightly wrong in one respect so I've had to overcome that. The site in question can be found here. You will notice in the top right that there is a login section with one field for username. Obviously you need two fields for a login so I've made it so that on the submit button click it brings up a ThickBox window with the proper login form. What I would like to have happen is that when a name is input into the form and submit is clicked, that value is automatically passed into the value of username in the thickbox window, when it pops up.
I have managed to edit the ThickBox.js with the following, so that the value for the member name is attached to the url and passed to the thickbox, but for some reason when I echo that value in php, nothing appears? Am I going about this the wrong way, or have I missed something?
I've added the above 3 lines just after:Code:var theMember = $('#formtext').attr('value'); url = url + '&member=' + theMember; alert (url);
And it will bring up an alert box when submit is clicked that displays whatever you submitted at the end of the URL, but getting it to appear in the ThickBox is causing problems!Code:if(url.indexOf('TB_iframe') != -1){// either iframe or ajax window
Thanks for any help!


text:
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Press Ctrl+Alt+Shift+S to bring up the Styles window. Click Manage Styles (third button at the very bottom of the window).
Edit the font settings for the entries below (for easy browsing, sort them alphabetically). For each, make sure that New documents based on this template is selected (near the bottom of the window).


text:
News Headlines
Clinton Scrutinized Over RFK Remark
By now, you've no doubt heard about the latest dust-up in the Democratic race for the White House: Hillary Clinton's invoking of the assassination of RFK, as a justification for her continued pursuit of the nomination.
She made the comment during an interview with a newspaper editorial board:
What's your opinion of her comments?
Related Links:
Clinton Again Seeks to Explain RFK Remarks
'Let's Move Forward', Axelrod Says of Clinton RFK Gaffe
You must be signed in to leave a comment. Sign In / Register
Please keep your community civil. All comments must follow the NPR.org Community rules and terms of use, and will be moderated prior to posting. NPR reserves the right to use the comments we receive, in whole or in part, and to use the commenter's name and location, in any medium. See also the Terms of Use, Privacy Policy and Community FAQ.
I think that Hillary Clinton says very little that is not calculated.
This nomination was Senator Clinton's nomination to lose. She has done a bang up job of turning people who cared for her, against her. Instead of worrying about the prsidency, she should worry about how this bid will impact her next attempt at maintaining her senatorial seat in New York. I can not imagine that she will be seen in the same way she was seen prior to this attempt to run for the presidency, by anyone. I am afraid that the bloom is off the rose and it may never return.
I'm done with her
please discuss Keith Olbermann's reaction on Countdown last week.
According to Matthew 12:34, Jesus said, "from the abundance of the heart, the mouth speaks." I do not believe this statement would have departed from the lips of Mrs.Clinton were they first not in her heart.


text:
I have a form where logged in users can upload a file to a folder. I want to make it so they can restrict access to downloading this file -so only logged in users can see the link to download the file.
But of course, if someone isn't logged in and happened to come across the url of the file, they can download it. Is there anyway to make it so that files can't be downloaded unless the user is logged in?
I came across a thread that said the only way to do something like this is to make it so the files are uploaded to mysql. Is this really the case and if so, does anyone know how this is done?
Thanks in advance for any help.


text:
Eurodam Cruise Review by cru1sn: what a disappointment!
Overall Member Rating
what a disappointment!
Destination: Western Caribbean
Embarkation: Fort Lauderdale (Port Everglades)
Seven day Thanksgiving cruise, western caribbean, weather iffy, which was no fault of theirs, but they could of made up for it with better food and service.
Biggest gripe..LINES, in the buffet on the lido, you waited for everything, coffee, eggs. bacon, etc. all in separate lines! No table service unless you managed to snag a seat in the "mariner" designated tables, which were few and far between! After the second day, we resorted to room service breakfast, which was delivered hot and promptly.
We had main seating in the dining room, at the back where they had a long walk to the kitchen, after the second night, we started to dine at Tamarind, one of the specialty restaurants, yes, an upcharge but at least the food was edible! And, the waitstaff were attentive and prompt, something that we lacked in the dining room. The dining room dinner menu consisted of two vegan and two regular entrees, most of the time, inedible! They even messed up turkey on Thanksgiving, a More medallion of turkey per person and lousy dressing and NO mashed potatoes, and the guests were mainly American!
I had cruised on the Eurodam the year before in Norway and enjoyed it, nothing spectacular, but it did not disappoint. Beware of the Eurodam, it's marginal at best! Less
Published 01/08/14
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Cabin review:
Amazing getaway for 2!
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What is the preferred way to store some data on the users computer for long periods of time. I am unsure how long a session lives. As an alternative I could store the data in a cookie, but here I can't find any high level storing API which also takes care of signing my data and make sure it is not tampered with.
How do you typically solve this problem of having a persistent user id between sessions.


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ETF Forecasts, Swing Trades & Long Term Investing Signals
Is Another Black August Looming?
July 27, 2016
GET MY ETF TRADES & INVESTING SIGNALS – CLICK HERE The post Is Another Black August Looming? appeared first on ETF Forecasts, Swing Trades & Long Term Investing Signals.
Insiders Are Secretly Selling
July 26, 2016
A Cycle Top In The Stock Market Next Month It was "Panic Buying" that pushed the Dow Jones and the SPX Indices to record highs. To be sure there is trouble brewing in this rally. Smart money has left the stock market, as the charts at the end of this article will display! While Wall […]
Don't Buy The SPX Hope Rally!
July 25, 2016
All bubbles burst; the question is when? Quantitative Easing (QE) is much like an addiction. One needs more and more to get the initial effect, however, this becomes an asymptotic result, whereas, eventually, one needs an infinite amount that will no longer give a positive effect! So, now that QE has failed, I believe there […]
Large investors become major buyers of Bitcoin
July 22, 2016
The world has woken up to the fact that the Central Banks are a curse, rather than a boom to the global economies, and their time left is slowly coming to an end because of new technologies and currencies I talked about last week. People are starting to park their money in digital currencies, like Bitcoin, […]
Insight from Greed, Volatility, and Put/Call Ratio
July 21, 2016
I was recently looking at the CNN money website where they post this fear & green index. I caught my attention because many other aspects of the market are now also showing signs an imminent correction in the stock market. This analysis is a contrarians play, meaning you believe that when mass majority of market […]
This Terribly Boring Process will Make You Money
July 21, 2016
This Is Just Like Buckley's Cough Medicine Slogan… "Tastes Terrible, But You Don't Need It For Long!" Last week I mentioned what I did years ago to get my trading and emotions in control so that I could trade for both weekly income and long-term account growth. I will be honest with you, it was not […]
SPY Spike Alert Triggered!
July 19, 2016
On Friday, July 15th our exclusive filtered SpikeAlerts service nailed another winning trade to lock in a quick and easy $399+ profit in less than 10 trading hours for subscribers. This signal was sent out at 5:02pm ET which members were advised to buy UPRO, SSO, or ES futures contract in post-market trading hours. I should […]
Keep an eye on 'Bitcoin' as the next 'financial crisis' starts!
July 18, 2016
'Bitcoin' is on a tear away rally. Its' performance, over the last year, has been outstanding and it has outperformed most 'asset classes', by a wide margin. It is probably the only asset class which beats out both gold and silver, in 2016. Why is it shooting into outer space? People look at alternate asset […]
This Weeks Market Forecast – SPX, Oil, Gold & Bonds
July 18, 2016
GET THIS VIDEO EVERY DAY BEFORE THE MARKET OPENS – CLICK HERE The post This Weeks Market Forecast – SPX, Oil, Gold & Bonds appeared first on ETF Forecasts, Swing Trades & Long Term Investing Signals.
The Long Wave "Winter of Discontent"!
July 14, 2016
I wrote and exclusive article for CNA Finance today which I want to share with you. It talks about the Kondratiev Wave. Just like all major long-term/big picture trends and cycle changes, nothing happens quickly. Its very much a process and the market will continue to move in a direction that makes no sense longer […]
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text:
- Please call us for FREE help:
- (866) 873-0030
Rensselaer, NY Senior Living
Columbia Care Home is an assisted living facility. Assisted living facilities are an apartment-style habitat designed to focus on providing assistance with daily living activities. They provide a higher level of service for the elderly which can include preparing meals, housekeeping, medication assistance, laundry, and also do regular check-in's on the residents. Basically, they are designed to (more...)
Eastwyck Village is a Senior Community. Senior communities are for seniors who can take care of themselves. It is often a set of apartments in a variety of sizes from studios to large two bedrooms, where seniors live independently, but may receive additional services like transportation, pools, a convenience store, bank, barber/beauty shop, resident laundry, housekeeping, and security, are all s (more...)
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text: Corn Products International (CPO) Q2 2011 Earnings Call July 28, 2011 9:00 am ET Executives Ilene Gordon - Chairman, Chief Executive Officer and President Aaron Hoffman - Vice President of Investor Relations & Corporate Communications Cheryl Beebe - Chief Financial Officer and Executive Vice President Analysts Vincent Andrews - Morgan Stanley Christina McGlone - Deutsche Bank AG Adam Josephson - KeyBanc Capital Markets Inc. Heather Jones - BB&T Capital Markets Christine McCracken - Cleveland Research Company Kenneth Zaslow - BMO Capital Markets U.S. David Driscoll - Citigroup Inc Presentation Operator Compare to: Previous Statements by CPO » Corn Products International, Inc. Q4 2009 Earnings Call Transcript » Corn Products International Inc. Q3 2009 Earnings Call Transcript » Corn Products International Inc. F4Q08 Earnings Call Transcript Good morning, everyone, and welcome to the Corn Products International 2011 Second Quarter Earnings Call. Today's conference is being recorded. At this time, I would like to turn the conference over to Aaron Hoffman. Please go ahead. Aaron Hoffman Thanks, Corinne. Good morning, and welcome to Corn Products Second Quarter 2011 Earnings Call. Joining on the call this morning are Ilene Gordon, our Chairman and CEO; and Cheryl Beebe, our Chief Financial Officer. Our results were issued this morning in a press release that can be found on our website, cornproducts.com. The slides accompanying this presentation can also be found on the website and were posted about an hour ago for your convenience. As a reminder, our comments within this presentation may contain forward-looking statements. These statements are subject to various risks and uncertainties. Actual results could differ materially from those -- predicted in those forward-looking statements, and Corn Products International is under no obligation to update them in the future as or if circumstances change. Additional information concerning factors that could cause actual results to differ materially from those discussed during today's conference call or in this morning's press release can be found in the company's most recently filed annual report on Form 10-K and subsequent reports on Form 10-Q and 8-K. Prev 0 of 3 Next


text:
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Portland's name was decided by a coin toss. The settlement's owners each wanted it to be named after his hometown. Portland, Maine beat out Boston, Massachusetts.
Portland, Oregon is the only U.S. city with a volcano within its city limits.
Portland is home to the world's smallest park, Mill End's Park, at just 24 inches across.
Don Wildman: At the turn of the 20th Century, America was home to one of the most dangerous, vice filled cities in the world, Portland, Oregon. Today it's among the country's most desirable places but it secretly harbors an underworld darker than you could possibly imagine. Whether its trap doors used to kidnap unsuspecting victims, the eerie recesses of a drug utopia, the lost stash of the city's bootlegging queen or even an illegal underground fight club, Portland has more dark corners than any medieval dungeon, and danger looms around every corner. The sins of Portland's past are alive and well, they're just buried beneath the surface.
S 3 : Ep 13
Aired 2/9/09
S 3 : Ep 12
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Aired 1/12/09
User Score: 485
User Score: 16
User Score: 12
User Score: 5
User Score: 5
User Score: 1


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Milwaukee Security Officer Solutions
Local Response Backed by National Support.
On April 8, Security Officers Ernest Johnson and Jason Knox received notification about a woman who had not paid bus fare. Upon their evaluation, it become clear that she was disorientated so they contacted the local police who confirmed she was a missing person. Our officers did an outstanding job of partnering with police and helping the woman return to the mental health facility where she resides.


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A Redverz - NICE! pretty jealous of that! SO you set it up in the garage, huh? I admit, the fly is a bit of a problem...
Type: Posts; User: cv66seabear
A Redverz - NICE! pretty jealous of that! SO you set it up in the garage, huh? I admit, the fly is a bit of a problem...
There's a forum for that? Well, of course there is, there's one for everything!
I found an ENO UQ on sale, but I know what you mean!
Read through some of the posts about questions setting up the kit, it's not hard, but it definitely takes a run through or two! I use...
Nice, I love tantos. Who is the maker?
Ok I missed something, I thought at first you said you could adjust it while you were in your rack - but you adjust it by taking up slack or releasing tension at the ring on either end?
Hi KM -
These pack in to a bag about 22" X 4" around. I know weight isn't an issue since I Moto-camp as well. This is about as long as a tent, but can be strapped on almost anywhere. I strap...
And Welcome from Wisconsin
Nice, I jjust can't buy another right now. Very fine blade, why can't I be one of those rich guys? I would have so many nice blades...
Good luck! You'll find someone who will appreciate a fine...
You can try these:
I think towards the beginning of Just Jeff's review of Handy Hammock there was someone who had another solution...
That's great! I wonder what the total number of members is? Jason?
I'll take it! It's just too nice to pass up! PM sent.
PM me in two weeks if you haven't sold it - I can't do it this paycheck! I love tanto blade knives. This is a very nice knife - Id love to own it.
Great Idea for a fundraiser. I think you should make sure this gets around to other troops.
You should have the older scouts do the whoopies and ridgelines (supervised, of course). Could be...
Here is the response from ENO regarding the Pro Straps:
Hi Kent,
Sorry to hear about your disappointment in the Pro Straps. Nylon does have a tendency to stretch, particularly the first few...
If you ever need toggles let me know - I have a local archery sport shop that will give me all the toggles I can use! Graphite and aluminum. I can send you a few?
I wrote to ENO tonight, will be interesting if they exchange the straps for a set of Atlas straps. I'm interested to see their response.
You should check out the new improved FF at Waupaca - a lot like Point!
Let's hang one day! Central Wisconsin Hang!
John - what a great offer (which I took you up on!) Thanks so much!
I bought the straps on sale at Gander Mountain a year ago, before I knew anything. I even bought the pro 10' ones. I didn't...
Must be coming soon...
I'm thinking of repairing it and adding a second layer to slip the pad in between. I would use the holy layer as the outside, and the fresh hammock as the inside.
I understand your concerns. ...
It was the first use of the ENOs as tree huggers, mabe I should have wrapped them twice.
Whoopies are on correctly, but I didn't milk my buries!
I have some tenacious tape I bought for tent...
Finally we have Spring here, so on my weekend Motorcycle tune up tech get together, I brought my new DIY (Tablecloth) hammock. This was my first hang and I broke the cardinal rule - I didn't try...
Welcome from Wisconsin!
Group buy through the 10th!
As a noob, I have to say that I have felt great about asking any question, no matter how ignorant. I can't wait to hang with you folks!
(To other noobs - get The Ultimate Hang by Derek Hansen -...
+1 on noobs! (Since I are one)
You could go to your local sport shop that cuts arrows and get some graphite or aluminum toggles free.
If you don't have a local shop, just pm me, and I'll send...
Gotta love autocorrect!
I hope this was a case of autocorrect! :)
+1 You couldn't have said it any better Floridahanger!
Thanks also to JustJeff and fuzzie for taking one on the backside!
Please post pics and your results of the "field test." I ordered mine today, bu would love a report.
From Jason:
Sorry for the group email - it's late here (3:30 am) :)
First off, wow! Thanks for your support/interest in Handy Hammock, it's
very humbling to see so much interest in our...
You are very welcome! It wasn't without some desire on my part to own this kit!
Hello All -
The Vendor Deal is on - 22 members committed in less than 24 hours to purchase the Handy Hammock sets.
Here's how to get in on the buy:
Email Jason at firstname.lastname@example.com...
Keep us posted!
True. This is a Vendor Sale, with me helping out with the posting (I get nothing for this, just like a group buy). I started this as a group buy, (on other forums this would still qualify),...
Definitely! - although I should have said MOMA - The Museum of Modern Art :D
Pesky house - always i the way!
Yep. Message me. We are 4 folks away from Jason's goal of at least 20 members. When we get there, I'll pm everyone who contacted me first with how to order, then make an announcement here for the...
In Jason's own words - "ALL NATIONS :)"
Come one, come all! We are nearly at 75%.
I know it's been set up with a Hennessy - I would think it will work with any camping hammock unless you have an exceptionally long hammock (over 11'). I know JustJeff tried it out.
I know that's...
We're just over halfway to the mark! Keep those cards and letters coming!
When we hit it, Jason will take over and everything will go through him.
I love your designs - some I have no idea what they do, but they belong in the MMA!
Here is JustJeff's review on the forum: https://www.hammockforums.net/forum/showthread.php?t=65826
I know what you mean. Good luck - we've already got 6 sets so I'm sure the buy will be on. I can see if Jason will go out another week, or so, too.
I appreciate it! THe buy is posted under the other vendors thread for Handy Hammock here:
The mods (quite rightly) didn't...
OK, here is what Jason can do within his budget for a "group buy." I am posting this here on behalf of Jason.
Please contact me by pm or email, and when 19 more folks agree to buy, I'll let...
Due to the nature of the buy, it will be offered as a deal on the Vendor page. I will set up the link and post the information for Jason at Handy Hammock.
Please give me a day to get this...
You should post some pics next hang! Sounds like a good way to control the tarp!
Nice sharpening system - still waiting for the "Eagle to poop" or I'd be interested. Becker is a nice knife, too.
So your tarp is pretty low and tight to your hammock?


text:
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Direct Phone: (203) ***-****
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Managing Member
Newberry Realty
Stanford Business School
CIRE Magazine Article
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Torrey D. Brooks, CCIM, vice president of Brooks, Torrey & Scott in Norwalk, Conn., uses a Xerox Phaser 850, which
ILWO - Westport Chamber of Commerce member directory
www.ilovewestport.com [cached]
Brooks, Torrey & Scott Torrey D. Brooks
Shawmut Bank v. Brooks Development (Connecticut, 1997)
www.fraudulenttransfers.com [cached]
One action was brought by Westfair, Inc., against Torrey D. Brooks, his wife Lauren F. Brooks, Connecticut National Bank (now Fleet Bank), Westport Bank and Trust Company, and Westbrook, Inc., to foreclose on a mortgage encumbering certain real estate. *fn1 The other action was brought by Shawmut Bank (now Fleet Bank) against Brooks Development Corporation (Development), Torrey D. Brooks (Torrey), Lauren F. Brooks (Lauren), Brooks, Torrey and Scott, Inc. (BTS), and Westfair, Inc. (Westfair), to collect on a promissory note, which was unsecured, and to set aside as fraudulent two mortgage conveyances, one of which was the conveyance at issue in the foreclosure action.
Member Directory
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Sue Brooks
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Heather Brooks
Emerald Coast Autism Center
John Brooks
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Jay Brooks
Woodlands Enterprises Inc


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