text:
The shooting death of Mike Brown, a black teenager, by a police officer in Ferguson. Mo., comes as the public, courts, and lawmakers have started debating limits to immunity and arguing about policies to recalibrate the role of police.
North Carolina voters without photo ID won't be turned away from the polls this November, but they will be asked to sign an acknowledgment of the new ID requirement – a step critics worry will make for longer lines to vote.
Eric Garner's death by illegal chokehold during an arrest for minor street crime is forcing Mayor de Balsio to come to terms with his campaign pledge to lower crime and improve relations between New York City police and poorer residents.
Theodore Wafer said he shot Renisha McBride on his front porch out of fear. The guilty verdict became another milestone in the US debate over liberalized gun rights and self-defense laws.
The D.C. Metro says Alex Young cannot leave his guitar case open for tips when he plays for commuters. His lawsuit says the open case sends a message to society, making it protected free speech.
Bankrupt Detroit has seen rising paranoia over race and crime, and the police chief has urged residents to arm themselves. Homeowner Theodore Wafer shot Renisha McBride through his porch door.
The convergence of cases could produce a massive showdown over gay marriage, as early as next year, when the cases are expected to be appealed to the US Supreme Court.
A federal judge on Monday ruled that an Alabama law violates women's constitutional rights by making abortions too hard to get. This follows a similar ruling last week about a Mississippi law.
The homicide ruling, along with simmering resentment in the city's poor neighborhoods over tough police tactics, will put pressure on prosecutors to level murder or manslaughter charges against police officers.
Digital currency lawsuits reveal weaknesses in a burgeoning industry with little regulatory oversight. Bitcoin is turning to third parties to reassure users that millions of dollars of value will not vanish without a trace.
The petition to the Supreme Court centers on text in the Obamacare law that appears to sharply restrict the provision of federal tax credits. Federal appeals courts issued opposing rulings on the issue last week.
Act 10 essentially ended collective bargaining for most public workers, sparking court challenges and protests. But a ruling Thursday by the Wisconsin Supreme Court leaves opponents with little choice but to move on.
A panel from the Fifth Circuit Court of Appeals ruled that a 2012 Mississippi law mandating clinic doctors have admitting privileges at local hospitals deprived women of their rights.
The Supreme Court upheld the individual mandate citing Congress's tax-writing authority, the lawsuit noted. But the federal appeals court said Obamacare's purpose is not to raise revenue, so it did not have to originate in the House.
Displaying the large steel cross pulled from the rubble of the World Trade Center did not promote religion, the court ruled. This cross is also 'an inclusive symbol for any persons seeking hope and comfort in the aftermath of the September 11 attacks.'
After a federal appeals court on Monday ruled against state gay marriage bans, North Carolina's attorney general said 'there are no more arguments to be made' against same-sex marriage. Not all in the Bible Belt agree.
The 2-1 ruling from the Fourth US Circuit in Richmond is the third in recent weeks by a federal appeals court striking down a state same-sex marriage ban, making it more likely the Supreme Court will take up the issue.
A federal judge released a decision Saturday that the Washington D.C. ban on carrying handguns outside the home is unconstitutional. Effectively immediately, registered D.C. gun owners may carry a firearm in public.
Some jails and prisons are pushing to sign up exiting inmates for Medicaid, courtesy of Obamacare. The idea is that if ex-prisoners receive regular health care on the outside, fewer will reoffend. Not all experts are believers, however.
The Florida law bars doctors from discussing firearms safety with their patients. It was passed after several individuals perceived questions about gun ownership as intrusive and offensive. An appeals court upheld the privacy law.
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text:
Arizona, country suffered entrepreneurial lapse, Brookings report says
PHOENIX — The land of opportunity appears to be turning into a wasteland for entrepreneurship.
According to the Brookings Institution, business dynamism plummeted in all 50 states and all but one of the 366 metro areas in the country during from 1978-2011. Only McAllen, Texas, has had more new businesses open than close.
Brookings defines business dynamism as "the process by which firms continually are born, fail, expand, and contract, as some jobs are created, others are destroyed, and others still are turned over".
In Arizona, more than twice as many businesses have shut down than opened their doors during that time period. Robert Litan with Brookings said his research group doesn't have a definitive answer as to why this is happening, but he does have a few theories.
"One may be that society is becoming more sclerotic (not being able to adapt). Big firms may be coming bigger. There may be more regulation that may be deterring new firms from forming," he said.
While he doesn't know of a definitive reason, Litan said he has the perfect solution for America to start churning out entrepreneurs once again: Immigration.
"Greatly liberalize our immigration laws to let in high-skilled immigrants — people who come here to study to get master's and PhD's in the so-called tech areas," he said. "(Another solutions is) to let in foreign entrepreneurs."
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subset: commoncrawl


text:
This question already has an answer here:
Should be Simple. I'm using Rails and doing something along the lines of:
<% n = 15 / 2 %>
<%= n %>
However, whenever I output variable n, it doesn't give me a decimal. Based on other calculations it looks like it's constantly rounding to floor, But I haven't added any sort of round method anywhere.
Could you explain why? I want it to actually round to ceil.


text: On May 19, 2004, at 3:15 PM, Omega wrote: > I just discovered Fire, and it is simply a great product. Plus, I like=20= > the "free" and open source oriented philosophy. Therefore, I'd like to=20= > contribute and translate this app into French, my mother tongue. > > I suppose that I need first to check if anyone is currently doing so,=20= > or has done it. I (as you see) subscribed to the localization mailing=20= > list, but I cannot reach the list archive, how can I do it ? I also=20 > got the localization how to, but I will wait for an answer before to=20= > start. > > Best regards, > No=E9 Falzon (aka omega, aka tenoch) As far as I know, no one is currently working on French. We did have a=20= localization of the 0.32.x series in French, which hopefully you could=20= use as a starting point for a delta localization with AppleGlot. The list archive is here: http://sourceforge.net/mailarchive/forum.php?forum_id=3D9789 -Jason=


text:
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text:
Skip to comments.Rasmussen poll gives McAuliffe a 17-point lead over Cuccinelli
Posted on 10/22/2013 11:25:05 AM PDT by Alter Kaker
A poll released Tuesday suggests Democrat Terry McAuliffe has pulled away in the campaign for Virginia governor, opening a 17-point lead against Republican Attorney General Kenneth T. Cuccinelli II two weeks from Election Day.
The poll, conducted by Rasmussen Reports, has Mr. McAuliffe ahead 50-33 by far the largest margin of any poll thus far in the race.
The survey of 1,000 likely voters has a 3 percentage point margin of error. It was conducted Sunday, a day after former Secretary of State Hillary Rodham Clinton campaigned with Mr. McAuliffe in Falls Church.
The results come after Rasmussen last month had Mr. McAuliffe with a 6 percentage point lead, 44 percent to 38 percent, over Mr. Cuccinelli. That poll was conducted before the two-week federal government shutdown, which other surveys have suggested had a disproportionately negative effect on Republicans.
(Excerpt) Read more at washingtontimes.com ...
What is wrong with VA that they would elect an absolute crook like McAuliffe?
Northern Virginia has been taken over by the Rat feces..VA is basically a done deal
Call it. Corey Booker didn't even have those kinds of numbers.
I hope Virginians enjoy getting ransacked by Fast Terry's crew. He'll strip it down like a chop shop in East Brooklyn.
Cuccinelli is losing because he's playing RINO - he didn't want to be photographed with Ted Cruz because it would scare the mushy moderates.
Americans know, a proven crooked Democrat is better than any Republican.
Hang onto your hats folks, 2014 is going to be bumpy.
What makes us think these numbers are fact-based? So much voter fraud, so little defense.
Cuccinelli was a fool to attack Cruz 3 weeks ago. The margin has exploded open since then.
Virginia turns permanently 'rat? Next: Florida. then: Texas.
Our Republic is dead.
Northern VA is a suburb of DC.
I don't watch television but friends have told me the democrats are running around the clock attack ads on the GOP since they have all that Hollywood $$ pouring in.
They want their own Owe Malley.
One day at a time.
Cuccinelli only needs to pick up 1.2 points each day between here and the election and he will win the race.
It can be done.
A bunch of money to precinct captains in Richmond and Norfolk.
If the tables were turned, this is the time some damaging personal information would be leaked about the front-runner. Not that I'm suggesting anything...
Good for them. They'll get what they vote for. A crook.
The only thing worse then a commie 'Rat is a commie wannabe RINO.
He is losing because VA is a purple state trending blue. We are outnumbered by the liberals—simple as that. And it doesn't help that the RINOs led by Bill Bolling are trashing Cuccinelli. A true conservative will have a very difficult time ever winning a statewide race in VA.
Virginia turns permanently rat? Next: Florida. then: Texas....
I'm afraid your right in Texas.
No one knows the Republican candidate for Governor except political junkies.
Never trust "The New Rasmussen." Scott sold out and I think he must have taken "his secret sauce" with him.
Immigration and domestic migration from the Northeast have changed the demographics of VA. It will be solid blue within a decade.
Time for more of Virginia to secede and make West Virginia bigger?
Why in the hell did Cuccinelli hire David Rexrode as campaign manager (worked for McCain) and Matt Rhoades for opposition research (Drudge BFF who was Romney's campaign manager)? They're both proven failures who hate grassroots conservatives.
I sincerely hope and pray that this poll is very wrong, beccause it would be a grave sin to elect McCauliffe.
Voters love candidates with damaging personal information. It makes them "humannnnnnnn". We are almost a one party nation at this point. The liberals own immigrants, gays, dark minorities, women, college students, media, and welfare recipients. That pretty much covers most of America except for white men and nobody cares about them until payday.
I grew up in Northern VA, it is now a cesspool of bureaucrats, liberals and hipsters, which all vote Communist.
VA is like CA, the population centers are all dependent or Liberal and the hinterlands all Conservative.
All dun.
Fund Obamacare with a tax on entertainment.
Sounds like Cuccinelli has the same RINO consultants that McCain and Romeny had.
It wasn't always the case. Remember when anti-ObamaCare warrior Cuccinelli used to come on Mark Levin's show every week? What a downfall.
You can't fix stupid!
"What is wrong with VA that they would elect an absolute crook like McAuliffe?"
Most everybody living in VA is either a crook or on the government payroll or sucking the government tit in another form.. It just a mater of preserving a way of life... not my or your kinda life but their way none the less.
The ones straight downhill are usually the smoothest.
Women voters
"Cuccinelli only needs to pick up 1.2 points each day between here and the election and he will win the race.
It can be done"
not when he is pretending to be Jeb Bush light
Hope the GOPe is watchin.......lots of RINOs are going down...keep an eye on what happens to the fat RINO gov...
He jumped the shark when he dissed Cruz.....
Hope the GOPe is watchin.......lots of RINOs are going down...keep an eye on what happens to the fat RINO gov in NJ...
Revolt is coming.
Giving Terry McAuliffe a seat in government is like giving an escaped death row convict a fully automatic rifle and a pallet of ammunition...
Why? Is Ken that bad of candidate? Did he have a Macaca moment? Is he running that bad of campaign?
[ He jumped the shark when he dissed Cruz.....
Hope the GOPe is watchin.......lots of RINOs are going down...keep an eye on what happens to the fat RINO gov... ]
The RINO fools will ALWAYS be in DENIAL, they will always blame their own downfall on the Tea party and never the fact that they are bunch of country club liberal minded elitists that cannot connect with the man on the street...
I see 2014 rather as the year the TP movement will really, really take off.
I doubt that had anything to do with it... it's more that Virginia has simply succumbed to the tidal wave of liberals from DC and MD. Demographics killed us.
[ Virginia turns permanently rat? Next: Florida. then: Texas.
Our Republic is dead. ]
That one way trip to Mars looks better and better every day....
Yep... conquered territory.
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text:
The very early beginnings of the UI for a new Codeschool course, launching in December!
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search for a card
Result of the search for the collectible card:
The following 12 users match with the requested search. Click on their names to contact them.
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Veteran Graves Honored by 'Wreaths Across America'
Veteran Graves Honored by 'Wreaths Across America'
119 results for 'maryland'. Showing results 37 - 54
Veteran Graves Honored by 'Wreaths Across America'
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text:
Stylish Design. Innovative Technology. Affordable Price.
Discover our capsule loyalty club. Join today and receive 2 free stamps on us!
For coffee-shop quality tea and coffee, with new, dedicated tea function
Enjoy a healthier lifestyle with fast, nutrient-rich juices
Part of traditional Easter fare, this classic Easter cookie is delicately spiced and lightly fragranced with the flavor of lemon peel. Try using biscuit cutters to cut into seasonal shapes of a rabbit, an egg or an Easter chick. A perfect teatime treat to serve alongside Simnel Cake.
Preheat oven to 200c. Lightly grease two baking sheets.
Using Food Processor (large bowl and dough blade) place all ingredients in bowl, except egg white and milk. Mix. Add sufficient milk to mix to a fairly soft dough.
Turn the dough to a floured surface, knead lightly until smooth, then roll out using a floured rolling pin to about 5mm thick. Cut the dough into rounds using a 5cm fluted biscuit cutter. Transfer the rounds to prepared sheets and bake for 10 mins.
Beat the egg white, then brush over the biscuits. Sprinkle with caster sugar and return to the oven for a further 10 mins, until golden. Transfer to a wire rack to cool.


text:
Warring States XDUniversal App – Optimized for iPhone and iPad.
Published by: Chen Hao
Price: FreeWas
$4.99Track this app for price drops.
- Current Version 1.50
- Size 9.87 MB
- Released Dec 16, 2010
- Age 9+
- Category Strategy
- User Ratings 3.50 (5 votes)
App Description
★★★★★ Free for limited time! Thanks for supporting us!
★★★★★ Big Sale! Download now! Thanks for supporting us!
★★★★★ You will get 80 Gems by logging in with this app for the first time!!!
This is our popular MMO RPG-Strategy game. You are a chieftain of a small village during the Shang period in China (1600 BC), and by making key decisions in research, commerce, city planning, and ultimately warfare, you can develop your village into a mighty empire. The game needs long-term strategy planning and RPG combat and is ideally suited to be played on mobile devices.
* Classic Simcity/ Civilization style build, research and expand.
* Easy step by step tutorials guide you in game.
* Train and promote general, forge your great army, fight with NPC and other players.
* Offline development, when you log out, your empire will continue to run based on your orders.
* Huge world map filled with quests, NPC, resource outpost, and mysteries waiting for you to explore and conquer!
* Great gears and items can be looted during battles.
* Quests for all levels of players, complete them for great rewards.
* Intelligent city attack/ defense system!
* Create or join clans to fight and win together with friends!
* Chat with other players online.
What's new in version 1.50
Bugs fixed.
Need help? Use our Warring States XD Walkthrough or sign up and make your contribution today.
Warring States XD is a universal app, and is available on the iTunes App Store for free with in-app purchases. The game is compatible with all devices running iOS 3.2 or later, including the new iPhone 6 and iPad Mini. To download the Warring States XD app click the button below to go to the app's download page.
If you've enjoyed this app, rate and review it on the App store and in the comments section below.
iPhone/iPad Screenshots
Customer Reviews
Please. Please bring this game back
This Game. Can not type amounts. Do not buy this game until this is fixed.
Amazing game BUT******. Can't enter amounts or type in game. Chat talk is useless and training is useless. Don't think this game is optimized for ios7.
Sent email to dev. Team couple days ago and no response. Uninstalled til
Historical characters!. Rare historical generals and leaders should be added to boost the games' enjoyment. Recruiting a rare general is an excitement for
all gamers! Beside creating your own general, there should be a tavern or something to find and recruit historical characters.
Completing quests to be able to recruit th
Sorry. That previous post was by my buddy who set up my iPhone account. Just told me he did that. He's just butthurt he can't afford gems
to play.
Share this Review
This review of Warring States XD was written by Carol Swift
Download Warring States XD App Latest Version on Android and iOS. You can get it directly on your device or download the app to your PC or Mac computer then install the app on your tablet or smart phone.
Related Apps
Age of Warring Empire
Warring States
Warring States Pro


text:
Integrated Modeling of Transport Phenomena in Fusion Liquid Metal Flows
Award Information
Small Business Information
Hypercomp Inc.
2629 Townsgate Road, Suite 105, Westlake Village, CA, 91361
Principal Investigator:
Ramakanth Munipalli
(805) 371-7500
(805) 371-7500
Business Contact:
Vijaya Shankar
(805) 371-7556
(805) 371-7556
Research Institution:
AbstractAccurate prediction of coupled transport phenomena is critical to the design of liquid metal blankets used in fusion reactors. While rapid advances are being made in the computer modeling of blanket systems in areas of fluid flow, magnetohydrodynamics (MHD), heat transfer, structural mechanics, and neutronics, there is a need for advancing the physical models of coupled transport processes (e.g., tritium permeation and corrosion of structural materials in liquid metals in the presence of a strong magnetic field). In particular, a capability is needed to integrate multi-species flow computations with multidimensional solvers, thus benefiting from the comprehensive modeling of flows consisting of two or more species, electromagnetics, and heat transfer. This project will build a solver for coupled transport phenomena in generalized geometries, and will account for momentum, heat, and mass transfer processes under liquid metal blanket conditions. Commercial Applications and other Benefits as described by the awardeeThe proper evaluation of transport processes in liquid metal MHD is vital to the design of breeding fusion blankets, and should lead to improvements in the safety and economic feasibility of fusion reactors.
* information listed above is at the time of submission.
An Official Website of the United States Government


text:
These two authors equally contributed to the work.
Original Article
Tryptophan hydroxylase 2 (TPH2) in a neuronal cell line: modulation by cell differentiation and NRSF/rest activity
Version of Record online: 28 SEP 2012
DOI: 10.1111/jnc.12004
© 2012 The Authors Journal of Neurochemistry © 2012 International Society for Neurochemistry
Journal of Neurochemistry
Volume 123, Issue 6, pages 963–970, December 2012
Additional Information
How to Cite
J. Neurochem. (2012) 123, 963–970.
Publication History
- Issue online: 4 DEC 2012
- Version of Record online: 28 SEP 2012
- Accepted manuscript online: 7 SEP 2012 06:10AM EST
- Manuscript Accepted: 1 SEP 2012
- Manuscript Revised: 29 AUG 2012
- Manuscript Received: 27 JUL 2012
Funded by
- Fondi di Ateneo
- Second University of Naples
- Grant-in-Aid for Scientific Research(C). Grant Number: 16591170
- Abstract
- Article
- References
- Cited By
- neural cells;
- serotonin;
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
Serotonin (5-HT) is a neurotransmitter involved in many aspects of the neuronal function. The synthesis of 5-HT is initiated by the hydroxylation of tryptophan, catalyzed by tryptophan hydroxylase (TPH). Two isoforms of TPH (TPH1 and TPH2) have been identified, with TPH2 almost exclusively expressed in the brain. Following TPH2 discovery, it was reported that polymorphisms of both gene and non-coding regions are associated with a spectrum of psychiatric disorders. Thus, insights into the mechanisms that specifically regulate TPH2 expression and its modulation by exogenous stimuli may represent a new therapeutic approach to modify serotonergic neurotransmission. To this aim, a CNS-originated cell line expressing TPH2 endogenously represents a valid model system. In this study, we report that TPH2 transcript and protein are modulated by neuronal differentiation in the cell line A1 mes-c-myc (A1). Moreover, we show luciferase activity driven by the human TPH2 promoter region and demonstrate that upon mutation of the NRSF/REST responsive element, the promoter activity strongly increases with cell differentiation. Our data suggest that A1 cells could represent a model system, allowing an insight into the mechanisms of regulation of TPH2 and to identify novel therapeutic targets in the development of drugs for the management of psychiatric disorders.
- cAMP
cyclic adenosine mono phosphate
Central nervous system
fetal bovine serum
Glial fibrillic acidic protein
hypothalamic–pituitary–adrenal axis
hypoxantine phosphoribosyl transferase
minimal essential medium
neuron-restrictive silencer element
neuron-restrictive silencer factor/repressor element-1-silencing transcription factor
neuron-specific enolase
polymerase chain reaction
Reverse-Transcription polymerase chain reaction
selective serotonin reuptake inhibitor
tryptophan hydroxylase
Serotonin (5-HT) is an important neurotransmitter involved in many aspects of the neuronal functions including the regulation of anxiety, depression, appetite, sleep, and circadian rhythm.
The synthesis of 5-HT is initiated by the hydroxylation of the amino acid tryptophan, which is the rate-limiting step catalyzed by tryptophan hydroxylase (TPH). Two isoforms of TPH (TPH1 and TPH2), encoded by different genes, have been identified with TPH2 almost exclusively expressed in the brain, whereas TPH1 is predominantly expressed in the pineal gland (Walther et al. 2003; Abumaria et al. 2008) and in the peripheral tissues (Malek et al. 2005; Turner et al. 2006; Nakamura and Hasegawa 2007). Genetic or epigenetic factors affecting TPH2 gene expression or catalytic properties may alter 5-HT neurotransmission and thereby modify behavioral traits, drug responses, and disease susceptibility. In fact, a loss-of-function mutation in human TPH2 gene was identified in patients with major depression (Zhang et al. 2005), and a loss-of-function mutation in mice was associated with a reduced aggressive behavior (Kulikov et al. 2005). Also, psychiatric disorders such as bipolar disorder (Lopez et al. 2007), attention deficit/hyperactivity disorder (Walitza et al. 2005), and suicidality (Zill et al. 2004), have been associated with TPH2 promoter polymorphisms. Moreover, Chen et al. also reported a functional polymorphism in the 3′ untranslated region of the TPH2 gene in rhesus macaques, which was related to differential hypothalamus–pituitary–adrenal (HPA) axis functioning, and, among peer-reared infants, was associated with the aggressive behavior (Zhang et al. 2004).
The expression of TPH2 in brain exhibits a circadian rhythm (Liang et al. 2004; Malek et al. 2005) and is influenced by specific stressors and hormones, such as sexual hormones suggesting that TPH2 gene expression may be frequently and subtly regulated under specific physiological or stress conditions. This distinctive feature of TPH2 supports the finding that 5-HT is involved in the function of the HPA axis, a critical neuroendocrine system that responds to stress and also shows a circadian rhythm. The dysfunction of this system is implicated in numerous psychiatric diseases. Thus, genetic polymorphisms or other cis-acting factors affecting TPH2 gene expression might result in the alteration of HPA axis function or other physiological processes related to 5-HT (Chamas et al. 2004; Zill et al. 2004; Clark et al. 2005; Leonard 2005; Sanchez et al. 2005; Brown et al. 2006; Hiroi et al. 2006; Chen et al. 2010).
Accordingly, mechanisms that specifically regulate TPH2 expression and its modulation by exogenous stimuli may be important for the understanding of the etiopathogenesis or management of psychiatric disorders (Chen and Miller 2012).
Recent studies have identified regulatory factors of serotonergic neuronal gene expression. These include the nuclear deformed epidermal autoregulatory factor-1, the Freud-1 and -2, and the neuron-restrictive silencing factor (NRSF) also known as repressor element-1-silencing transcription factor (REST) (Patel et al. 2007; Albert and Francois 2010). Patel et al. described a functional motif in the TPH2 promoter that is a novel bipartite variant of the binding site for NRSF (Patel et al. 2007). It is well known that NRSF limits tissue expression of neuronal genes through the canonical 21-bp motif called neuron-restrictive silencer element (NRSE) and is implicated in neurogenesis (Tateno et al. 2006), neuronal differentiation (Abrajano et al. 2009), and transcriptional regulation of a network of genes. NRSF represses transcription of its target genes by recruiting a silencing complex of chromatin-remodeling proteins, variably including SIN3A, histone deacetylase, CoREST, and methyl-CpG-binding protein 2 (Schoenherr and Anderson 1995; Abrajano et al. 2009).
In this study, we addressed the issue of the modulation of endogenous TPH2 expression in undifferentiated and differentiated neural cells and, in particular, of the characterization of TPH2 human promoter and its molecular regulation. For this purpose, we used a mouse mesencephalic embryonic cell line, A1 mes-c-myc (A1) cells. A1 cells were immortalized by means of infection with c-myc carrying retroviral vector of early neural precursors (E11), thus these cells express markers belonging to different neural cell lineages including neural stem cells such as neuron-specific enolase (NSE), glial fibrillic acidic protein (GFAP), and nestin. These cells proliferate and remain undifferentiated when grown in the presence of serum, whereas they cease to proliferate and differentiate, ensuing neurite outgrowth, neuronal electrophysiological properties, and expression of neuronal markers when serum is withdrawn and cAMP is added (Colucci-D'Amato et al. 1999).
In a previous study, we characterized, by means of RT-PCR, the expression of serotonergic markers in the A1 cells before and after differentiation. In particular, we demonstrated that A1 cells express the transcripts of the two rate-limiting enzymes, TPH1 and TPH2, necessary for serotonin synthesis, and that the two enzymes were differently expressed in proliferating and differentiated cells. We also found that TPHs were modulated by fluoxetine and citalopram, two selective serotonin reuptake inhibitor (SSRI) drugs widely used in therapy (Di Lieto et al. 2007). In Fig. 1 are represented the main features of undifferentiated/proliferating A1(uA1) cells and differentiated/non-proliferating A1(dA1) cells.
Materials and methods
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
Cell cultures
A1 cells were generated in our laboratory immortalizing, by means of c-myc infection, a culture obtained from embryonic mesencephalon of CD1 mice at day 11 (Colucci-D'Amato et al. 1999)
A1 cells were cultured and differentiated as previously reported (Colucci-D'Amato et al. 1999). Briefly, A1 cells were cultured in minimal essential medium (MEM)/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and were differentiated by serum withdrawal and stimulation with 1 mM cAMP (Sigma Aldrich) and N2 supplement (Invitrogen).
CD1 male mice (Charles River, Milan, Italy) were used for the study as they belong to the strain used to obtain A1 cells. The animal facility was maintained at 21°C with a reversed 12 h:12 h light/dark cycle (lights off at 08.00 a.m.). After arrival, animals were habituated to the conditions for 10 days and handled daily. Animals were lightly anesthesized with isofluorane and killed between 10:00 and 11:00 a.m. Animal experiments were conducted in accordance with the European Council Directive of November 24, 1986 (86/609/ECC).
Eight CD1 male mice (18–20 weeks of age) were decapitated, brains were carefully removed, and the pineal glands were quickly removed and frozen at −80°C. Brains were dissected into two parts by cutting perpendicularly to the cortical surface, dorsal raphe nuclei (DRN) were rapidly removed and immediately frozen over liquid nitrogen and stored at −80°C until use.
RNA isolation and real-time PCR analysis
Isolation of the total RNA from cells and tissues was performed using the TotalRNA isolation Kit (Promega, Paisley, UK) according to the manufacturer's instructions. In particular, 1 × 106 adherent cells were rapidly harvested by manual scraping in the presence of RNA Lysis buffer®. Brain tissues frozen in liquid nitrogen were ground using a mortar and pestle, and RNA Lysis buffer® was added to the ground tissues.
cDNA was synthesized from 1 μg of total RNA using SuperScript II reverse transcriptase (Invitrogen) and random hexamer oligonucleotides. cDNA was then used for real-time PCR. Forward (f) and reverse (r) primers used were (5′–3′) TPH1 f GGCTTGCTTTCTTCCATCAG; r ATGGAGAGAGGGCGAGAGACA. TPH2 f GCAGCCCGCAATGATGATGT; r GCACGTTGTCTTCCCTGTAG. HPRT f CCTGCTGGATTACATTAAAGCACTG; r CCTGAAGTACTCATTATAGTCAAGG; NRSF f GTCTCAGTGGTCCAGGTAAGTA; r CCTTTTGTCCGTCTGT G.
Amplification reactions were run in triplicate in MicroAmp 96-well plates (Applied Biosystems, Carlsbad, CA, USA) containing 5 μL cDNA, 2 μL of primers mix (final primer concentration of 300 nM each), and 12.5 μL of Power SYBR Green PCR Master Mix (Applied Biosystems) in a final volume of 25 μL. Amplifications were carried out in an ABI PRISM® 7900 Sequence Detection System (from Applied Biosystems). To activate the Taq Polymerase, reactions started with two initial steps of 50°C for 2 min and 95°C for 10 min. The reaction proceeded with 40 cycles of 95°C for 15 s and 60°C for 1 min for gene amplification. A final dissociation step was always performed to obtain the melting curves (thermal profile) of the amplicons obtained in the reactions. mRNA levels were expressed as 2−ΔCt using hypoxantine phosphoribosyl transferase (HPRT) mRNA level as internal standard.
Protein analysis
Isolation of cytosolic protein was performed by scraping cells in lysis buffer (25 mM Tris HCl, pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.1 mM NaF, 0.1 mM Na3VO4). Protein concentration was determined by the Bradford method. Forty micrograms of protein extract was subjected to a 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and electroblotted on nitrocellulose membranes, which were blocked at room temperature for 1 h in blocking solution [5% milk in Tris-Buffered Saline Tween 20 (TBS-T)], incubated overnight at 4°C with anti-TPH1 or anti-TPH2 antibodies (1 μg/mL; Abcam, Cambridge, UK). Membrane was incubated with peroxidase-conjugated anti-rabbit (for TPH1) and anti-goat (for TPH2) antibodies (1 : 10 000; Amersham Biosciences, Piscataway, NJ, USA). Proteins were revealed by an ECL kit (Amersham). Normalization was performed with anti-beta actin antibodies (1 μg/mL in TBS-T; Tansduction Lab., Lexington, KI, USA) for 1 h at 25°C and incubated with peroxidase-conjugated anti-mouse IgG (1 : 10 000 in TBS-T). Proteins were revealed as above. The intensity of the bands was quantified by scanning densitometry using Scion Image version 4.5 software (Scion Corp., Frederick, MD, USA).
Preparation of reporter constructs TPH2-55 and TPH2-9
A genomic clone covering the human TPH2 gene promoter region (GenBank accession No.; AC090109) was isolated from the human genomic library (Clontech, Mountain View, CA, USA). The 8.7-kb Bam HI/Bam HI fragment (−8755 to −2; adenine of the translation start codon was assigned to +1) was cloned into the Bgl II-digested pGL4.10 [luc2] (pGL4-Basic) (Promega), giving TPH2-23. Then, TPH2-23 was digested with Xho I/Afl II to generate TPH2-55 containing a 2-kb fragment upstream of the translation start site (−1990 to −2). TPH2-55 has a potential non-canonical NRSE sequence (5′-TTCAGCACCAGGGTTCTGGACAGCG CC-3′, −133 to −107) (Patel et al. 2007). To make an NRSE-disrupted mutant, TPH2-55 was used as the template for PCR using QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) and mutation primer (5′-gggaggattcgcattgctcTTCAGCAGTTTTCTGGACAGCGCCccaagc agg-3′; upper case letters indicate disrupted NRSE). The resultant mutant was referred to as TPH2-9 having a 3-bp (CAC) deletion and a 2-bp (GG to TT) substitution in the NRSE.
Transient transfection analysis of the TPH2 gene promoter activity
A modified transfection protocol was used to analyze promoter activities in differentiated A1 cells in transient transfection experiments (Troelsen et al. 2003).
A1 cells (2 × 105) were cultured in 24-well tissue culture dish containing 0.5 mL of proliferating medium (MEM/F12, 10% FBS) for 2 days. Transfection was performed by using 2 μg of the reporter construct (TPH2-55 or TPH2-9) or the molar equivalent amount of the pGL4-Basic and TransIT-Neural (Mirus, Milton Keynes, UK) reagent, and by incubating A1 cells at 37°C for 5 h according to the supplier's protocol. Then, all culture medium was removed, and 0.5 mL of fresh differentiation medium (MEM/F12, 1XN2, 1 mM cAMP) was added to each tissue culture dish. Incubation was continued for additional 43 h, 67 h, 115 h, and 163 h (namely 2, 3, 5, and 7 days after transfections) under differentiating conditions.
Medium was changed every 2 days. Cells were harvested at each time point and luciferase activities were measured using a dual luciferase assay system (Promega) with Lumicounter 700 (Microtec. Co. Ltd., Chiba, Japan). In some experiments, A1 cells (8 × 104) were cultured in 24-well dish containing 0.5 mL of proliferating medium for 2 days. After transfections as described above, 0.5 mL of fresh proliferating medium was added and A1 cells were incubated for additional 43 h (namely 2 days after transfections) under proliferating conditions to measure luciferase activities.
Each reporter construct was cotransfected with 0.1 μg of the phRL-TK (Promega) to correct transfection efficiencies. Total amount of transfected DNA was adjusted to 2.1 μg by adding pUC 19, if required. TPH2 promoter activities are expressed as fold changes relative to luciferase activities in cells transfected with pGL4-Basic (set at 1.0) and represent mean ± SEM of five separate experiments; each was performed in duplicate.
In all the experiments, statistical significance was determined using the two-tailed t-test.
RNA silencing
dA1 cells were transfected with NRSF shRNA and scrambled shRNA (OriGene, Rockville, MD, USA), using Turbofection8 solution according to the manufacturer indications. Transfected cells were cultivated in MEM-F12 10% FBS and incubated in 5% CO2 incubator for 48 h before harvesting for RNA analysis.
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
TPH1 and TPH2 are differentially expressed in uA1 or dA1 cells
In this study, we investigate TPH1 and TPH2 expression in A1 cells as both mRNA and protein levels, by means of quantitative real-time PCR and western blotting, respectively.
As shown in Fig. 2, real-time PCR analysis shows that TPH1 mRNA expression is higher in uA1 cells as compared with dA1 cells. On the other hand, TPH2 mRNA is more expressed in dA1 cells as compared with uA1. TPH protein expression, performed by western blotting analysis on the total protein extracts obtained from uA1 and dA1 cells, confirmed that TPH1 is significantly more expressed in uA1 cells as compared with dA1 cells (Fig. 3). As far as TPH2, we also show that it is more expressed in dA1 than uA1 cells (Fig. 3). These results were quantitatively confirmed by densitometric analysis of the protein signals normalized to beta-actin expression. Protein extracts from mouse dorsal raphe nuclei and mouse pineal glands were used as positive controls and protein extracts from NIH3T3 cells were used as negative controls (Fig. 3).
NRSF mRNA is more expressed in uA1 than dA1 cells, and the TPH2 gene promoter activity increased in dA1 cells
To better understand how TPH2 expression is regulated in A1 cells, we analyzed, by means of real-time PCR, the expression of NRSF, a zinc-finger transcription factor involved in the repressions of the neuronal genes in neuronal progenitor cells (Schoenherr and Anderson 1995). As shown in Fig. 4a, we observed that NRSF is significantly more expressed in uA1 cells as compared with dA1 cells. To further clarify NRSF role in the regulation of TPH2 gene expression in uA1 and dA1 cells, we cloned a 2-kb fragment upstream of the translation start site of the human TPH2 gene into pGL4-Basic, giving TPH2-55. It was recently reported that the promoter fragment of 2-kb length relative to the transcription start site (corresponding to −141) conferred almost complete specificity to serotonergic neurons (Chen et al. 2009; Chen et al. 2009). This suggests that TPH2-55 (−1991 to −2) has essential control sequences for the expression of the human TPH2 gene in serotonergic neurons. Moreover, it was reported that TPH2 gene has a functional NRSE in the vicinity of its transcription start site (Benzekhroufa et al. 2009, Patel et al. 2007). On the basis of these findings, we generated an NRSE-disrupted construct TPH2-9 to efficiently analyze the NRSF-mediated regulation of the TPH2 gene promoter activity (Fig. 4b).
Reporter constructs were transfected in proliferating conditions and then A1 cells were further incubated either in proliferating (for 2 days) or differentiating (for 2, 3, 5, and 7 days) conditions to measure luciferase activities (Fig. 4c). TPH2-55 gave a luciferase activity about 1.5-fold higher than that of pGL4-Basic, suggesting that the promoter activity of TPH2-55 was distinct but low. The luciferase activity of TPH2-55 remained nearly constant for the whole period of observation (2–7 days of differentiation). Concomitantly, TPH2-9 gave about three-fold higher activity as compared with pGL4-Basic in proliferating conditions (Fig. 4c, 2#), and the luciferase activity of TPH2-9 progressively increased in differentiating conditions, reaching about a five-fold higher activity at 7 days after induction of differentiation (Fig. 4c, 2–7). Finally, to definitely clarify the role of NRSF in the regulation of TPH2 expression, we performed NRSF knock down in uA1 cells by means of shRNA transfection. As shown in Fig. 4d, NRSF knock down in uA1 cells is able to enhance TPH2 mRNA expression, whereas the transfection with a scrambled shRNA did not affect TPH2 mRNA expression in uA1 cells.
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
In this study, we confirm by different approaches and extend the findings that A1 cells, a neuronal immortalized cell line from embryonic mouse mesencephalon, express the rate-limiting step enzymes necessary for 5-HT synthesis: TPH1 and TPH2. In particular, in this article we show, by means of real-time PCR and western blotting analysis that A1 cells are able to express both TPH1 and TPH2 mRNAs and proteins, and that this expression is dependent on cell proliferation/differentiation status. TPH1 is more expressed in undifferentiated cells, whereas TPH2 expression is higher in differentiated cells. This finding is in line with the well-known observation that TPH2 is almost exclusively expressed in the brain, whereas TPH1 is predominantly expressed in the pineal gland and the peripheral tissues (Malek et al. 2005; Turner et al. 2006). Thus, our findings demonstrate that A1 cells in their differentiated status are able to turn enzyme expression from non-neural TPH1 isoform to the neuron-specific TPH2 isoform.
Actually, TPH1 is expressed preferentially during the late developmental stages in the mouse brain, whereas TPH2 is expressed in the adult brain (Nakamura et al. 2006). Importantly, undifferentiated and differentiated A1 cells reproduce this alternative pattern of expression of TPH1 and TPH2 enzymes. Therefore, A1 cells, with their opposite regulation of TPH1 and TPH2 expression, may provide a useful tool to study the relevance and the molecular mechanisms underlying this switch.
Furthermore, it is also conceivable that A1 cells could represent an ideal model system to study 5-HT enzymatic pathway. Indeed, expression of recombinant protein in Escherichia coli is indispensable and has been widely used to obtain purified TPH1 and TPH2 in large quantities for further detailed structural and functional analysis. However, mammalian cell culture systems can provide conditions for protein expression, which are closer to the in vivo environment. To date, pheochromocytoma PC12 cells have been established as a model system to study TPH2 regulation and function when TPH2 is exogenously expressed (Wang et al. 2002). Moreover, TPH2, as well as other members of the superfamily of aromatic amino acid hydroxylases, forms homotetramers. Previous studies using recombinant TPH1 revealed a tendency of the purified proteins to form aberrant oligomers and aggregates, a phenomenon which may or may not occur in vivo (Yang and Kaufman 1994; Clark et al. 2008). Therefore, the use of mammalian cell systems that endogenously express TPH2 can provide a rapid functional analysis mimicking the in vivo condition. In contrast to the growing number of genetic association studies, little is known about the regulation of TPH2 expression. Limited in vivo studies suggest that TPH2 mRNA levels are decreased following stress corticosteroids (Lunyak and Rosenfeld 2005; Clark et al. 2005) and increased by ovarian steroids (Sanchez et al. 2005; Bethea et al. 2011), or hypotensive stress (Brown et al. 2006). For this reason, we focused our attention on TPH2 in A1 cells and aimed to better clarify the mechanisms underlying its expression in A1 cells.
Recently, Patel et al. identified a functional bipartite NRSE in the TPH2 gene promoter and observed that this particular NRSE permits TPH2 gene transcriptional repression by NRSF and that TPH2 mRNAs were up-regulated by dominant-negative NRSF in rat C6 glioma cells (Patel et al. 2007).
We analyzed the expression of the NRSF and TPH2 mRNAs by real-time PCR in A1 cell system. We show that NRSF mRNA is more expressed in uA1 cells than dA1 cells and, inversely, TPH2 mRNA is more expressed in dA1 than uA1 cells (Figs 2 and 4a). These results suggest that NRSF-mediated repression of the endogenous TPH2 gene transcription was, at least partly, relieved via a decrease in NRSF levels.
To further clarify the role of NRSF in the regulation of TPH2 gene promoter activity in uA1 and dA1 cells, we prepared an NRSE-intact (TPH2-55) and an NRSE-disrupted (TPH2-9) reporter construct to efficiently analyze the NRSF-mediated regulation of the TPH2 gene promoter activity. If endogenous TPH2 gene expression is regulated by NRSF binding to NRSE, TPH2-55 reporter activity is expected to be directly correlated with changes in endogenous TPH2 gene expression levels in dA1 cells. As shown in Fig. 4c, however, the luciferase activity of TPH2-55 remained low, even though higher than that of pGL4-Basic, and nearly constant in spite of a decrease in NRSF levels, implying that the reporter promoter activity does not necessarily correlate with the endogenous TPH2 gene expression. At this moment, the cause of this apparent discrepancy remains unclear, but might relate to the fact that the transiently transfected DNA lacks its proper chromatin structure and is thought to be more accessible to binding of factors (Hebbar and Archer 2008). We also could not exclude the possibility that additional as yet unidentified long-range regulatory elements may be involved in the regulation of the endogenous TPH2 gene transcription in dA1 cells.
It should be emphasized that luciferase activity of the NRSE-disrupted construct TPH2-9 progressively increased depending on A1 cell differentiation (Fig. 4c). These results show that dA1 cells indeed express transcription factors necessary for an activation of the TPH2 gene promoter in a differentiation-dependent fashion. These results also support the idea that A1 cells are useful to identify these potential transcription factors, which may play a pivotal role in regulation of the TPH2 gene expression in central serotonergic neurons.
More importantly, we demonstrated that NRSF knock down in uA1 cells are able to enhance TPH2 mRNA expression, definitely clarifying the role of NRSF in the regulation of TPH2 expression (Fig. 4d). Recently, it was reported that NRSF mRNA expression in dorsal raphe neurons was significantly increased in female subjects affected by major depressive disorder compared with matched controls, indicating that an alteration in NRSF expression may be associated with diminished 5-HT neurotransmission and the higher incidence of depression in women (Goswami et al. 2007).
It is now evident that genetic or epigenetic factors affecting TPH2 gene expression or catalytic properties may alter 5-HT neurotransmission and thereby modify behavioral traits, drug responses, and disease susceptibility. In particular, based on genetic studies, there is accumulating evidence that altered function of TPH2 in the brain plays a role in anxiety- and depression-related personality traits (Zhang et al. 2005). Findings from psychophysiological and functional imaging studies are indicative of various TPH2 polymorphisms directly influencing serotonergic function and thus impacting on mood disorders and on the response to antidepressant treatment (Zill et al. 2004; Walitza et al. 2005; Lopez et al. 2007).
Moreover, it is worth noting that, we have recently published the proteomic profiling of both uA1 and dA1 cells (Chambery et al. 2009). Data mining and comparative proteome analysis can be used to identify protein networks involved in TPH2 regulation and may provide a tool to discover molecular biomarkers of drug action and novel therapeutic targets.
In conclusion, our data suggest that A1 cells could represent a model system able to allow a deep insight into the mechanisms of regulation of TPH2 gene expression and to identify novel therapeutic targets in the development of more useful drugs for the management of psychiatric disorders.
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
We thank Dr. Antonio Di Lieto (Napoli) for helpful comments, Dr. Elvira de Leonibus (Napoli) for providing mice, Mafalda Giovanna Reccia (Caserta) and Yoko Okada (Kawasaki) for technical support.
Financial disclosure
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
This work was supported by "Fondi di Ateneo", Second University of Naples to LCD and by Grant-in-Aid for Scientific Research(C) (16591170) to HM.
The authors reported no biomedical financial interests or potential conflicts of interest with regards to this manuscript.
- Top of page
- Abstract
- Materials and methods
- Results
- Discussion
- Acknowledgements
- Financial disclosure
- References
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Crazy Substitute Teachers
Enjoy, NG4.03 / 5.00 1,880 Views
Vacuum Salesman
This dude and his Megazord vacuum cleaner4.00 / 5.00 2,540 Views
Markiplier Animated | Mark Of The Survey Corps
This took far too long to make...3.90 / 5.00 4,862 Views
kinda odd, but pretty cool animation tho :)
Why thank you Kovskin...I'll take that as a compliment:)
Please update the file and put action script stop(); at the end. The teaser is kinda slow , the music is cool so is the animation , can't wait to see the release of it.
Thanks...I will update that..I love frame by frame "old school" animation:)
some quality key frames in some of the battle movements
i'm glad you liked it!:)
= I guess they call it a "Teaser" for a reason.
Seems to look promising, my assumption is that this will be an action adventure, The animation Templates are pretty good, but nothing makes sense, even for a trailer, if there were other sprites involved it would of sparked more curiosity for the viewer.
I look forward to seeing the final product.
Thats a great response ...I was trying to get a feeling for the overall world at first...now I can add in some new animation for th final...Thanks!!:)
The label epic is right! It was. The game idea looks awesome but two things... One I thought at first the green bug was the bad guy because it didn't look like a fly but that is the samurai fly. So that confused me. Second, don't samurais have swords?
Ya Ya! This is a dragonfly bug before he become a adult dragon fly.His final look he will probably have many swords:)


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Mae Carlos
Published: Wednesday, November 11, 2009 at 8:57 a.m.
Last Modified: Wednesday, November 11, 2009 at 8:57 a.m.
Mae Ella Marie Carlos, 70, a native of Chauvin and resident of Houma, died at 12:40 p.m. Monday, Nov. 9, 2009.
Visitation will be from 9 a.m. to funeral time Thursday at St. Joseph Catholic Church. Service will be 11 a.m. Thursday at the church, with burial in the church cemetery.
She is survived by two daughters, Jodi Carlos Trahan and Kayla Leigh Carlos, both of Houma; one brother, Floyd Dupre and wife, Carolyn, of Bourg; five grandchildren, Veronica Vail, Maverick Boudreaux, Raven Boudreaux, Ty Theriot and Meekel Boudreaux; and four great-grandchildren, Wesley, Erin, Jude and Averyn.
She was preceded in death by her husband, John Joseph Carlos; her parents, Clifton Francis Dupre and Mildred Thibodaux Dupre; three brothers, Russell Dupre, Clifton Dupre Jr. and Leroy Dupre; and one sister, Agnes Smith.
She was a wonderful wife and mother. Although she didn't birth her children naturally, they were conceived in her heart long before they were born.
Chauvin Funeral Home is in charge of arrangements.
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Official Sonic the Hedgehog store opens in Europe
An official online store for Sonic the Hedgehog merchandise in Europe has opened, SEGA and RHM Solutions have announced.
Sonic and Me
Sonic the Hedgehog is the reason I got into gaming. I'm not trying to over-dramatise that, as I'm sure if it wasn't for him there would be some other 90s mascot that triggered my lifelong hobby. But for me it was Sonic, and that means something.


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Fiona Apple Announces Concert at Fillmore Miami Beach on September 30
Finding success in the music industry is hard. Pleasing both critics and commercial fans is even more difficult. Doing it all, plus setting a Guinness World Record by the time you're 23 is not only an achievement, it's an awesome feat. And apparently it sends you into hiding.
That's the oversimplified story of Fiona Apple, rock 'n' roll's mad poetess of the late '90s and queen of feminine angst. But the good news is she's coming out of her second self-imposed hiatus with a new album, The Idler Wheel. And she also just announced that she'll be bringing her elusive self to the Fillmore Miami Beach on September 30.
Apple's fourth studio album is the much-anticipated follow-up to her controversial Extraordinary Machines. The new record was produced in secret. Not even her record label knew of the sessions until she showed up with the final product earlier this year.
The Idler Wheel, which goes on sale today, has once again received critical acclaim, almost across the board. But the most exciting part is the chance to catch her live at the Fillmore this fall.
Upcoming Events
TicketsSat., Jul. 30, 7:00pm
Sociedad Proarte Grateli: Aquellos Tiempos Felices-La Habana De Los 50
TicketsSat., Jul. 30, 7:15pm
Laura Pausini
TicketsSat., Jul. 30, 8:00pm
Pitbull: The Bad Man Tour
TicketsSun., Jul. 31, 7:00pm
Olivia Holt
TicketsSun., Jul. 31, 7:00pm
If her return to the stage at this year's SXSW is any indication, Apple's upcoming Miami Beach performance should be awesome, emotional, artistic, and kinda crazy.
Fiona Apple. Sunday, September 30. Fillmore Miami Beach, 1700 Washington Ave., Miami Beach. The show starts at 8 p.m. and tickets cost $34.50 to $65.50 plus fees via livenation.com. All ages. Call 305-673-7300 or visit fillmoremb.com.
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"Lola Basyang" is the pen name of Severino Reyes, founder and editor of the Tagalog magazine Liwayway. From 1925, Reyes wrote a series of stories for children under the title Mga Kuwento ni Lola Basyang (The Stories of Grandmother Basyang). The original magazine stories have been adapted for comics (komiks), television, the cinema, and published in book form. Wikipedia
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"A small masterpiece of political theory with implications far beyond the educational context."--Suzanna Sherry, University of Texas Law Review
"Gutmann has created a theory of extraordinary coherence, comprehensiveness, and depth."--Alison M. Jaggar, The Philosophical Review
"Dr. Gutmann attempts to construct a democratic theory of education with great conceptual clarity and good common sense.... Democratic Education belongs in a liberal tradition that goes back to the birth of the Republic.... It is a tradition that began with the founders and is represented today by people like Thomas Nagel and John Rawls. It is the America de Tocqueville celebrated."--The Times Higher Education Supplement
"Amy Gutmann has written a courageous book.... One of Gutmann's most remarkable successes is her ability to sustain a principled argument through a dazzling range of issues of great moment and complexity."--Herbert M. Kliebard, Academe
"[Democratic Education] is unusual in offering a specifically political theory of education.... [The theory] is rigorously deployed and its practical implications are conscientiously demonstrated in close, well-documented and instructive discussion of controversial issues in the politics of American education."--Jean Floud, The Times Literary Supplement


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CHARLOTTE GRAY (PG-13) A well-intentioned but largely ineffective romance, this vehicle for Cate Blanchett stars the ubiquitous Aussie as a principled, patriotic Scotswoman who becomes a spy in Occupied France during WWII. While Australian director Gillian Armstrong may aspire to present a feminist heroine, the film's obsession with Charlotte's romantic entanglements makes this endeavor feel conventional. --Felicia Feaster
ORANGE COUNTY (PG-13) Lawrence Kasdan's son Jake directs Tom Hanks' son Colin in a comedy about an ambitious high-schooler who panics when Stanford University rejects his application. The cast includes Jon Lithgow, Lily Tomlin and Shallow Hal's Jack Black, who fuels the comic relief for the inevitable road trip.
VA SAVOIR (PG-13) Legendary 73-year-old French director Jacques Rivette presents a romantic roundelay among a group of theater people and other Parisian sophisticates. At two-and-a-half hours, this soft-spoken comedy isn't exactly riveting, but it makes wise observations about the conflicts of the heart and features thoughtful actors, especially Jeanne Balibar as a neurotic thespian. As it progresses, the script takes increasingly melodramatic turns, until it begins to resemble a stage play. Why? As the title translates, "who knows?" --CH
Duly Noted
ANNA WUNDER In this bittersweet coming-of-age story, the 12-year-old title character struggles to care for her neglected little brother and her alcoholic mother. Introduced by director Ulla Wagner. New Films From Germany, co-sponsored by the Goethe-Institut Atlanta. Jan. 11 at 8 p.m., Rich Auditorium, Woodruff Arts Center. $5.
GHOST WORLD 1/2 (R) Terry Zwigoff follows his documentary on cartoonist R. Crumb with a sharp feature based on Daniel Clowes' comic book serial about hip best friends (Thora Birch and Scarlet Johansson) who drift apart after high school. The film hilariously shows young people faced with the insipid mediocrity of consumer culture vs. the loneliness of personal authenticity, embodied by Steve Buscemi as a hapless record collector. The kind of film David Lynch or Woody Allen should be trying to make, Ghost World draws ideal performances from its leads while refusing to offer easy solutions to their dilemma. GSU's cinefest, Jan. 4-10. --CH
JAY & SILENT BOB STRIKE BACK (R) Kevin Smith provides a light-hearted coda to his "New Jersey" trilogy of films with this low brow, cameo-heavy road movie that boasts some hilarious spoofs on classic and current films. The foul-mouthed Laurel & Hardy team of Jay (Jason Mewes) and Silent Bob (Smith himself) offers a few gay jokes too many, but if the film isn't as good as Pee Wee's Big Adventure, it's at least better than Beavis & Butt-Head Do America.GSU's cinefest, Jan 4-10. --CH
MONTY PYTHON AND THE HOLY GRAIL They're Knights of the Round Table. They dance whene'er they're able. They do routines and chorus scenes with footwork impecc-able. It's a busy life in Camelot. They sing from the diaphragm a lot. (This re-release boasts a whopping "24 seconds" of hitherto unseen footage.) GSU's cinefest, Jan. 11-18. --CH
A NIGHT OF SHORTS The Atlanta Screenwriters Group presents the premiere screening of four short films from its members -- "The Last Day in November", "e-date", "I Love You..." and "The Walkers" -- as well as two trailers for upcoming features. Jan. 9 at 8 p.m., The Fountainhead Lounge, 485 Flat Shoals Ave.
THE RECKLESS MOMENT Remade as The Deep End with Tilda Swinton, Max Ophul's suspense film finds Joan Bennett as a mother being blackmailed by a suave cad (James Mason), who begins to fall in love with his victim. Films at the High, The Incomparable James Mason film series, Jan. 12 at 8 p.m., Rich Auditorium, Woodruff Arts Center. $5.
THE ROCKY HORROR PICTURE SHOW (R) The cult classic of cult classics, the 1975 horror spoof follows an all-American couple (Susan Sarandon and Barry Bostwick) to the castle of Dr. Frank-N-Furter (Tim Curry), a drag-queen/mad scientist from another galaxy. It's all fun and games until Meatloaf gets killed. Dress as your favorite character and participate in this musical on acid. Fridays at midnight, Lefont Plaza Theatre, 1049 Ponce de Leon Ave., and Saturday at midnight at Blackwell Star Cinema, 3378 Canton Road, Marietta.
ALI (PG-13) Director Michael Mann focuses on a single, tumultuous decade in the life of Muhammad Ali, from his championship bout against Sonny Liston to his refusal of the U.S. draft to "the Rumble in the Jungle." The film's first hour, placing the prizefighter's life in the context of America's racial and religious unrest, is as stinging and nimble as the boxer himself. A bulked-up Will Smith captures Ali's trash-talking and, more surprisingly, his moments of silent resolve, but neither Smith nor Mann can keep the film's last hour from losing dramatic interest, meticulously re-creating a fight whose outcome we already know. --Curt Holman
AMELIE (R) A popular and critical hit in France, this not-to-be-missed sweet-as-pie, stylistic knockout is a dazzling live-action cartoon for grown-ups. The ultra-cute Audrey Tautou is a do-gooding sprite living in a magical Montmartre who dedicates herself to helping others. From Jean-Pierre Jeunet, the director of The City of Lost Children and Delicatessean. --FF
A BEAUTIFUL MIND 1/2 (PG-13) In an either bold or ignorant move, director Ron Howard may have made the first action-adventure film about schizophrenia. Russell Crowe stars in this story of real life Princeton mathematician John Nash who won the Nobel Peach Prize, but also suffered from mental illness. Howard allows emotional button-pushing to triumph over character development and insight in this earnest but flat entry in Hollywood's disability canon. --FF
BEAUTY AND THE BEAST (G) The only animated feature ever to be nominated for a Best Picture Academy Award -- and one of the best classic-style musicals of the past 20 years -- Disney's 1991 animated gem gets a polish to fit the scalle of a really, really big IMAX screen. Mall of Georgia IMAX Theater, I-85 at Buford Drive, Buford.
BEHIND ENEMY LINES 1/2 (PG-13) One-note ubiquity Owen Wilson, a head-scratching choice for Hollywood's latest flavor of the month, plays Chris Burnett, a pilot shot down over ravaged, corpse-strewn Bosnia, which the film treats with the sensitivity of a video game. Director John Moore makes his movie debut after helming zippy commercials, so expect lots of choppy splicing of scenes filmed in the grainy style popularized by Saving Private Ryan -- but made dull by the number of hacks who have shamelessly copied it. --Matt Brunson
BLACK KNIGHT (PG-13) Sort of "A South Central Homeboy in King Arthur's Court," Martin Lawrence goes medieval as a theme park employee zapped back to the 14th century. Lawrence gets mileage of okay jokes like his pseudonym "Sir Skywalker," but he'd do well to emulate Eddie Murphy and go for a quiet laugh every once in a while. Look for Atlanta actor Dikran Tulaine, who has few lines but plenty of screen time, as one of the rebels. --CH
THE BUSINESS OF STRANGERS 1/2 (R) This assured, sleek debut film from Columbia grad Patrick Stettner is far more than just a girlified retread of Neil LaBute's corporate melodrama In the Company of Men. Stockard Channing astonishes as a driven, steely post-menopausal executive who shares her grievances about work and a life sacrificed for the almighty surge of power with an underling (Julia Stiles), who goads her to take violent action. --FF
GOSFORD PARK (R) As close to a masterpiece as this year in movies has seen, Gosford Park invests a familiar upstairs-downstairs theme of the upper and servant classes of English country life with a degree of compassion and sensitivity that proves director Robert Altman has something human lurking beneath his patented misanthropy. --FF
HARRY POTTER AND THE SORCERER'S STONE (PG) The big screen version of J.K. Rowling's first Harry Potter book mostly enchants. Following the title character (Daniel Radcliffe) in his first year at a Hogwarts School of Witchcraft and Wizardry, the film has so many clever details and visual delights that they dwarf the supposed plot about sinister goings-on surrounding the titular stone. John Williams overdoes the "magical" music and Radcliffe underplays Harry's emotions, but the film has enough charms to make the already-planned sequels feel welcome. --CH
HOW HIGH (R) If you're a fan of rappers Redman and Method Man, or are simply nostalgic for the cinema of Cheech & Chong, you might want to sample this comedy about two stoners who get into Harvard thanks to an IQ-boosting supply of marijuana -- which quickly runs out. With Fred Willard as the chancellor.
IMAX Lost Worlds: Life in the Balance (Not Rated) Harrison Ford narrates an IMAX film exploration of the world's biological diversity, from the Poles to the Tropics, with an in-depth focus on the lush, remote plateaus of Venezuela.Through March 22. Majestic White Horses (NR) The pomp, history and legend of the famous Lipizzan horses of Austria and the Spanish Riding School of Vienna gets the really big screen treatment. Through May 23. Fernbank Museum of Natural History IMAX Theater, 767 Clifton Road. --Steve Warren
IMPOSTOR (PG-13) Based on a Philip K. Dick story of the same name, this high-tech take on The Fugitive finds Gary Sinise as a futuristic engineer accused of being an alien spy. Featuring Madeleine Stowe and Vincent D'Onofrio, the film has literally spanned a millennium, having seen its theatrical release postponed for nearly two years.
IN THE BEDROOM (R) A thoughtful, admirable, first-director effort from actor Todd Field (Eyes Wide Shut), this story of how two chilly, noncommunicative Maine WASPS cope with their only son's death has a writerly attention to character, nuance and human behavior. Though Field's representation of his characters' suffering can at times feel a little meta-Bergman and precious, this timely story of grief and the search for revenge has enough application to the current social climate to make it resonate. --FF
JIMMY NEUTRON: BOY GENIUS (G) An accident-prone boy inventor comes to the rescue when aliens kidnap his parents in a computer-animated, big-screen version of the Nickelodeon cartoon. Voice actors include Martin Short, Andrea Martin and Patrick Stewart.
JOE SOMEBODY (PG) His work as Buzz Lightyear aside, Tim Allen is the new Steve Guttenberg, a bland actor whose generic films keep getting bankrolled. Allen's disrespected corporate employee gets punched out by a co-worker, and declares that he wants a rematch, which suddenly earns him love and respect (is this a multi-million dollar corporation or an elementary school?) You know you're in trouble when you're actively waiting for Jim Belushi to make an appearance in a movie. --MB
KATE & LEOPOLD 1/2 (PG-13) Meg Ryan, whose ceaseless attempts to remain the pixie queen of frothy romantic comedies are becoming embarrassing, plays Kate, an ambitious sales executive who falls for a 19th century Duke (Hugh Jackman, making another career misstep) transported to present-day New York. After an insufferable first half in which we watch Leopold predictably become perplexed by modern-day gadgets, the second half marginally picks up thanks to the pleasing presence of Breckin Meyer as Kate's good-natured brother. --MB
THE LORD OF THE RINGS: THE FELLOWSHIP OF THE RING (PG-13) Adapting the first and longest book of J.R.R. Tolkien's trilogy, Peter Jackson offers an all-but-perfect fantasy epic that's no simple piece of story-book escapism. The titular fellowship (played by a superbly cast ensemble including Elijah Wood and Ian McKellan) contend with evil forces as journey across land of Middle-Earth to destroy a magic ring. Jackson offers a full immersion in an imaginary world, and even when some virtual environments look fake, they bristle with personality. Thrilling -- and exhausting -- at a full three hours, Fellowship's greatest achievement is that it never loses sight of the human side of its fanciful story. --CH
THE MAJESTIC (PG) Jim Carrey makes another bid for respectability in director Frank Darabont's fantasy set in the McCarthyite 1950s. Carrey plays Pete, a blacklisted, amnesiac screenwriter mistaken for a long-lost WWII vet in a small town. The first part of the movie, dealing with Pete's involvement with the town's perpetually chipper residents will strike some viewers as inspiring and others as manipulative, while the final act, centered on Pete's stand against the House Un-American Activities Committee, whitewashes a tragic chapter in U.S. history. --MB
MONSTERS INC. (G) Remember the Warner Bros. cartoons in which the coyote and sheepdog would carry lunchpails and punch time clocks? Pixar's latest feat of computer animation employs a similar blue-collar incongruity, depicting the endearing monsters who scare children, and the chaos that erupts when a human kid gets loose in their world. The script isn't as solid as the Toy Story movies', but the creepy-crawlies come in wildly imaginative shapes and hues, and the climactic chase is excitingly surreal. With the voices of John Goodman, Billy Crystal and Steve Buscemi.
NOT ANOTHER TEEN MOVIE (R) The ferocity with which director Joel Gallen and his five writers deconstruct and devour the teen flick deserves some respect, as the film includes letter-perfect take-offs on everything from the John Hughes oeuvre of the 80s right up to the student-skewering hits of today. But for all its eager-beaver zeal to deliver raunchy laughs, the film provides the gross-outs but not the gags -- at least, not enough good ones to make it anything more than a quickie toss-off. --MB
OCEAN'S ELEVEN 1/2 (PG-13) There are a number of ways Steven Soderbergh could have botched his replay of Lewis Milestone's tongue-in-cheek 1960 Rat Pack Heist film. But Soderbergh shows, yet again, his flexibility when it comes to tackling a new genre, and an ability to adapt the winking cool of the original to the swingers generation. Hip, funny and laid-back, the only thing missing is an explanation for why former-indie-with-a-vision Soderbergh is so hung up on recrafting old genre material and rehashing others' work. --FF
OUT COLD (PG-13) No one I recognize is in the commercials, but supposedly Lee "The Six Million Dollar Man" Majors is on hand for this slovenly teen comedy about snowboarding and other "extreme" pastimes.
THE ROYAL TENENBAUMS 1/2 (R) Director Wes Anderson maintains the idiosyncratic brilliance of Rushmore with a pixilated portrait of an ingenious but dysfunctional family. The likable cast centers around Gene Hackman's rascally paterfamilias, but it's more a film about its textures -- nearly subliminal sight gags, wall-to-wall pop tunes from the '60s and '70s, fictional locales in an idealized Manhattan --- that subtly and overtly suggest the experience of reading a novel, especially the kind John Irving likes to write. --CH
SHALLOW HAL (PG-13) A nerd (Jack Black) only interested in physical beauty is "de-hypnotized" to perceive a 300-lb woman as a svelte beauty (Gwyneth Paltrow in a convincing fat suit). Most of the fat jokes in the Farrelly Brothers' winning romantic comedy have been crammed into the trailer, allowing the rest of the movie to make its case as a sympathetic tale about getting past surface appearances. Black's performance is a delight, retaining his character's goofball persona while also showing us the blossoming adult underneath, but Paltrow's empathic contribution is also key. --MB
THE SHIPPING NEWS 1/2 (R) Lasse Hallstrom's adaptation of E. Annie Proulx's Pulitzer Prize-winning novel is thankfully more in the tradition of the director's low-key What's Eating Gilbert Grape than his unctuous Chocolat. Kevin Spacey stars as a haunted man with a dead wife and a small daughter on his hands who returns to his family home in Newfoundland to start afresh. Hallstrom treats the subject of myriad family dysfunctions with sensitivity, but the historical sweep and plethora of characters eventually prove unmanageable. --FF
SIDEWALKS OF NEW YORK 1/2 (R) Ed Burns' stab at grown-up filmmaking is Jiffy Pop cinema: just add heat to an assortment of tried and true conventions, most of them cribbed from the self-derivative Woody Allen. Burns uses a faux-documentary form to chart the romantic ups and downs of a variety of Manhattanites who make you care less about their bedroom antics. If you're that desperate for pretentious sex play, just rent Husbands and Wives again. --FF
SPY GAME (R) If you enjoyed Tony Scott's sleek but smart efforts Crimson Tide and Enemy of the State, then this one's a good bet as well, mixing hard-hitting thrills with a decidedly less-than-benevolent look at U.S. government agencies. Robert Redford makes the most of his best role in years as a veteran CIA operative who learns that his former protege (Brad Pitt) is due for execution in China, and uses every trick in the book to save his neck. --MB
VANILLA SKY 1/2 (R) His soundtrack selections still fascinate, but writer-director Cameron Crowe flair for dialogue fails him in this ambitious, unsatisfying remake of Spain's Open Your Eyes. Tom Cruise plays a callow magazine publisher who loses his grip on reality after a disfiguring car accident. The film's ideas about love, dreams and mass entertainment pull the plot in opposing directions, while its resolution has been used in too many sci-fi scenarios. Cameron Diaz and Penelope Cruz both give vivid performances, though why Cruise's role so prefers one to the other is one of the film's unanswerable questions. --CH
THE WASH 1/2 (R). If you're not already a fan of Dr. Dre and Snoop Dogg, you'll probably find their comedic effort a wash out. Friday scripter DJ Pooh wrote and directed this nearly plotless look at the tension between two friends when they both take jobs at the same scruffy L.A. car wash. A few laughs lurk in the margins -- Pooh giving himself the funniest role as a dimwitted kidnapper -- but the cast has a much better time than the audience eveer will. Tommy Chong, Pauly Shore, Shaquille O'Neill and Eminem provide cameos. --CH


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August 22-24 2016 Vienna, Austria
Stem cell treatments for Parkinson's are still in the Initial stages of development. stem cell treatment is advanced treatment for many diseases like Alzheimers,parkinson and other neurological disorders.Stem cells have the ability to change into other type of cells. Because stem cells they can change into neurons,bone,muscle and other type of cells. Many researchers they believe that these stem cells have the ability to cure many diseases. Researchers are using stem cells to grow dopamine-producing nerve cells in the invitro condition, and these Dopamine producing nerve cells can transplanted into patients.
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Elvis Presley's Birth Certificate Up for Auction at GracelandInvaluable.com, who will host the auction, estimates that the birth certificate will sell for between $80,000 and $100,000.
Elvis Presley's Stepbrother Claims His Death was No AccidentDavid E. Stanley tells a dark tale in his new book 'My Brother Elvis.'
This Day in Music History - June 9thThis is what interesting stuff happened on today's date in music history.
This Day in Music History - May 24thThis is what interesting stuff happened on today's date in music history.
63 Best Laughs in Pop MusicWhile there's countless songs that feature a good laugh, both canned and totally off the cuff, here are the 63 that we think deserve some recognition.
U2 Brought Out an Elvis Impersonator On Stage Due to Technical Difficulties: WatchThey ended up performing "Can't Help Falling in Love."
Jack White Bought Elvis Presley's First Recording, Plans to Reissue for Record Store DayRecord Store Day will occur April 18 this year.
Let the Bidding Begin on Elvis Presley's Planes, Janis Joplin's VestA new year, new opportunities for bidding on celebrities' possessions.
'Elvis & Nixon' Film to Star Michael Shannon and Kevin SpaceyWhat went down in their secret meeting? We may never know, but the film 'Elvis & Nixon' will no doubt have fun speculating.
See Intimate, Previously Unreleased Photos of Elvis PresleyThe book is a collector's edition with limited copies — in total, only 1,706 were produced, all numbered and signed by Wertheimer.
Barbra Streisand 'Partners' with John Mayer, Stevie Wonder, Elvis and More for Duets AlbumThe legendary singer will also feature Blake Shelton, Lionel Richie and John Legend on her upcoming release.
Here's a Map That Reveals the Best-Selling Artist From Each StateBilly Joel rules New York. Prince has got a lock on Minnesota. But who takes hold of Washington? Hint, it's someone who's not nearly as cool as Nirvana.


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Video Industry News Video Company News Technology Industry News Technology Company News Novelty Industry News Novelty Company News Gay Industry News Gay Company News Legal Industry News All Company News
Released: Apr 03rd, 2007
Running Time: 119
Director: Rob Spallone
Company: VCA Pictures
Cast: Wanda Lust, Angelica Sin, Dick Delaware, Ginger Spice (II), Julie (I), Buster Good, Dave Pounder, Jenner, Cara Lott
Critical Rating: AAA 1/2
Genre: Specialty
Does anyone actually believe Wanda Lust could be a surgeon who "needs to relax" before her debut by balling the anesthesiologist in the operating room? Don't bother to answer; neither does Spallone — but he shot is anyway. Despite blunderbuss scenarios like this, MILF Shakes manages to entertain the crotch while numbing the brain.
At least the ladies are the right age. In Cara's case, this sexy Hall of Famer's back and better than ever, deep-throating Christian, eating his butt and orgasming during a tasty sidesaddle ride.
Also rounding the mountain is Julie, a moderately plump redhead, who loves to have Jenner tug her labia ring while slamming her rectum. She rewards him with a squirt!
As for technique, too many shots are unnecessarily overexposed — unacceptable for pops.
Retailing: Four hours of comps on bonus DVD!


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Stalin looks at you and thinks you are a pile of shit … Maybe he's right!
24 January, 2012
Stalin looks at youPosted by stalinsmoustache under Stalin | Tags: pile of shit |
Leave a Comment
24 January, 2012
Create a free website or blog at WordPress.com. — The Connections Theme.


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Million-dollar winner
The New York Lotterys Gretchen Dizer presented over-sized checks totaling $5 million to a group of recent Long Island Lottery winners each of whom won on a different Lottery game. Glenn Marrus of Plainview tells Newsday how he won his prize. Videojournalist: Barry Sloan (Jan. 30, 2014)
North Massapequa house fire kills 1 at scene, pedestrian injured in nearby accident involving responders Suffolk DA: 14 arrested in drug bust Still feeling the pain at anniversary of TWA Flight 800 Pro-police rally held in Selden Black Lives Matter marches held in East Hampton, Bridgehampton Latino Long Islanders protest Rep. Zeldin How to explore East End like a local Nun from LI reported missing in Austria Surveillance video shows car crashing into Patchogue restaurant DA: 31 indicted in huge hit to LI drug trade Dowling: No deal with Global University Systems LI principal drives summer bookmobile


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I have a client's site that needs to be redesigned, but I need to pull certain pieces of the old site into the new one...but don't have full access to all the code. I can get most of the JS, but need to incorporate it into the new site...on a different server.
Also have some CSS work to tie it all together.
Please email me at email@example.com with rates and previous work, if available.


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Gifts sought for local seniors
BARRE Santa and the Central Vermont Council on Aging could use help.
The council delivers hot holiday meals to seniors on Christmas Day and wants to include a gift with every meal.
The council is looking for shoeboxes filled with small items like slippers, socks, winter hats, pretty soaps, lotions, note cards, small decorative items, mugs, potholders and nonperishable food.
Those giving a box can either wrap it themselves or have the council wrap it. The council asks those donating to mark whether their gift is for a man or a woman. Financial donations are also accepted to help pay for the meals.
Shoeboxes or donations can be delivered or mailed to the council at 59 N. Main St., Suite 200 in Barre.
For more information, call the council at 479-0531.MORE IN This Just In
WESTMINSTER — A new Vermont State Police dispatch center in Westminster... Full StoryBARRE A Granite City man already facing multiple burglary charges is now facing more charges... Full Story
COLCHESTER — Police in Vermont have identified a Milton man who died after... Full Story
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Calm down.
Don't get excited.
Calm down.
Don't get excited.
Just in case you UD kiddies forgot, this is virtual reality. Things aren't always what they seem. There are trolls everywhere; including the UD admins that love to toy with you because they have your basic information acquired from your email and IP address. Are you assuming the worst in your BFs, GFs, or BFFs? If so, cool your jets. When there's a nerd that you love to hate because you assume they stabbed you in the back (and the front), think again. They might actually be a true friend that's tried their damnest to have your back (to no avail), but since there's life outside of the internet, stuff slips through the cracks. Have mercy! :D
by walking on eggshells October 10, 2013
Trending Now
Top Definition
It means to slow down, control your excitement (see hold your horses)
Cool your jets, your order will be ready in an hour.
by Bob L March 30, 2004
Swing dancing term used by vintage life-stylists to mean be calm or settle down
Jeez, cool your jets, it's not that big a deal.
by $ally August 04, 2007


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Watford graduates from Air Force basic training
Published: Monday, April 21, 2014 at 4:14 p.m.
Last Modified: Monday, April 21, 2014 at 4:14 p.m.
Air Force Airman Christopher A. Watford graduated from basic military training at Joint Base San Antonio- Lackland, San Antonio, Texas.
The airman completed an intensive, eight-week program that included training in military discipline and studies, Air Force core values, physical fitness, and basic warfare principles and skills.
Airmen who complete basic training earn four credits toward an associate in applied science degree through the Community College of the Air Force.
Watford earned distinction as an honor graduate.
He is the son of Shirley Erde of Boiling Springs and Phil Watford of Chesnee.
The airman is a 2010 graduate of Chesnee High School.
Call, email or mail Ashley Dill at:
-- 864-562-7272
-- email@example.com
-- Town Square, Herald-Journal, P.O. Box 1657, Spartanburg, SC 29304
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