text:
|Publication number
|Publication type
|Application number
|Publication date
|Nov 23, 1982
|Filing date
|Jun 12, 1981
|Priority date
|Jun 12, 1981
|Fee status
|Publication number
|Tipton L. Golias
|Original Assignee
|Helena Laboratories Corporation
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (2), Referenced by (24), Classifications (9), Legal Events (4)
|External Links: USPTO, USPTO Assignment, Espacenet
In the diagnosing of ailments of persons and animals, it is known that much information can be provided by an analysis of certain biological fluids such as serum proteins, lipoproteins, hemoglobin and isoenzymes. Electrophoresis as a method of separating the respective ingredients of said fluids, for a microscopic analysis or employing optical densitometry is known and shown or disclosed in one or more of the following patents:
______________________________________UNITED STATES PRIOR ART______________________________________3,607,645 Schneider 9/21/713,759,773 Dwyer et al 9/18/733,808,118 Golias 4/30/743,873,433 Seidel, et al 3/25/753,884,764 Goodhue 5/20/753,907,642 William Richmond 9/23/753,907,645 William Richmond 9/23/753,912,610 Kingdon Lou 10/14/75BRITISH PRIOR ART______________________________________1,060,874 Stanton (1966)1,211,008 Elevitch (1967)1,212,844 Stanton (1967)1,385,319 William Richmond (1972)1,385,320 William Richmond (1972)1,466,040 Behringwerke (1975) Aktiengesellschaft______________________________________
In the basic method of electrophoresis, charged molecules of fluids are separated under the influence of an electrical field wherein the sample of solution to be examined is mounted upon a support medium having a buffer moistened porous surface. Since the various components move at different rates, laterally through the support media, the sample may be separated into its respective elements. The subsequent staining of the fractions prepare the plate when dried for examination by optical densitometry or other methods.
Copending herewith is U.S. patent application Ser. No. 273,162, filed June 12, 1981, executed of even date, entitled Automated Electrophoresis Apparatus and Method.
An important feature of the present invention is to provide an automated electrophoresis and staining apparatus wherein an electrophoresis plate has applied thereto a sample for electrophoretic fractionization and supported in an upright position by a transfer frame so that the plate may be projected downwardly into an electrophoresis chamber for a predetermined period within an electrophoretic circuit.
A further feature is the provision in conjunction with the electrophoresis chamber of a plurality of solution containing vats arranged in a line upon a platform such that the plate may be transferred to a vat containing a stain solution and successively to other processing solutions, at all times maintaining the plate in an upright position.
A further feature includes a plate holder rack which has a horizontal frame adapted for supporting one or a plurality of electrophoresis plates in registry with the underlying chamber and vats.
A further feature is the provision of an electrophoresis chamber having a hinged cover within which the plate holder rack and the plates are supported which includes an electrical circuit with conductors and the use of conductive buffer moistened sponges in registry with the top and bottom edges of the plates for completing an electrical circuit through the electrophoresis plates.
A further feature includes a power operated lift and transfer assembly adapted to progressively lift, transfer and lower the plate holder rack and the plates from the chamber and progressively into each of the underlying vats for a predetermined period successively in a linear stepping motion, with the plates maintained at all times in an upright position.
A further feature is the specific electrophoresis chamber wherein an electrophoretic circuit is provided upon the interior thereof protectively enclosed by a pivoted cover and after a predetermined period, a power operated lift and transfer assembly on elevation automatically opens the cover so as not to block the upward movement of the rack and plates suspended therefrom.
A further feature is to provide automated electrophoresis and staining apparatus which includes the process of electrophoresis and necessary sample staining into a continuous operation.
A further feature of the present invention of the timing of the electrophoresis process may be predetermined depending upon the sample being fractionated wherein the voltage applied may be modified for a particular electrophoresis depending upon the nature of this sample tested. The interval of immersion within the respective vats may be preset according to a predetermined program or may be manually controlled.
A further feature includes a method of electrophoresis wherein there is included the step of supporting in an upright position a nonconductive plate having on one surface a buffer moistened electrophoresis media to which has been applied a sample selected from the group consisting of serum proteins, lipoproteins, hemoglobin and isoenzymes. Enclosing of the plate within an electrophoresis chamber after applying an electrical potential to the plate ends within an electrical power circuit for a predetermined period at a predetermined voltage, wherein the specimen elements fractionate and migrate laterally through portions of the media and after staining, is available for analysis by optical densitometry or other methods.
These and other objects will be seen from the following specification and claims in conjunction with the appended drawings.
FIG. 1 is a partly sectioned fragmentary side elevational view of the present automated electrophoresis and staining apparatus.
FIG. 2 is a vertical section taken in the direction of arrows 2--2 of FIG. 1.
FIG. 3 is a vertical section taken in the direction of arrows 3--3 of FIG. 2.
FIG. 4 is a fragmentary section taken in the direction of arrows 4--4 of FIG. 2 showing the plate holder rack.
FIG. 5 is a perspective view of the plate holder rack as further shown in FIG. 2.
It will be understood that the above drawings illustrate merely a preferred embodiment of the invention including the apparatus and the method employed, and that other embodiments are contemplated within the scope of the claims hereafter set forth.
The present automated electrophoresis and staining apparatus, is generally indicated at 11, FIG. 1 and includes an elongated base 13 upon which are mounted a series of longitudinally spaced transverse brackets 15 secured by fasteners 19 to the base and including mount flange 17, FIG. 2.
Spaced above said base and mounted upon the respective support arms 21 of said brackets is an elongated platform 23 suitably secured thereto.
Mounted upon platform 23 is a plastic container 25 defining an electrophoresis chamber 27 adapted to contain a suitable buffer solution 29 and having cover 31 hinged thereto at 33.
During the period of electrophoresis within chamber 27, cover 31 remains in a closed position as retained by the resilient hold down clips 35 anchored at 37, FIG. 2. The cover remains closed for a predetermined interval and thereafter is automatically moved to the open position shown in dash lines in FIG. 1 when the plate support rack 73 is elevated by a lift and transfer mechanism to which it is attached. One end wall 39 of the electrophoresis chamber is notched at its upper end at 41 through which a portion of the plate holding rack 73 projects as in FIG. 2.
A series of vats, preferably constructed of an inert material, such as a plastic, are mounted upon platform 23, side by side and arranged in a row and aligned with chamber 25. These vats are adapted to contain respectively a liquid stain as in the first vat 45, a first rinse solution in vat 47, FIG. 1, a second rinse solution in vat 49, a third rinse solution in vat 51, a fixative solution in vat 53 and a final rinse solution in vat 55.
The electrophoresis chamber 27 within container 25 suspends along the undersurface of cover 31, a buffer moistened conductive sponge 57 connecting the cover as at 59, FIG. 1 and in contact with and overlying an elongated platinum electrode 61 connected by lead 63 to a suitable power source.
Within a quantity of buffer solution upon the bottom of said container 25, there is provided a buffer moistened conductive sponge 65 retained upon the vertically adjustable elongated sponge support 67. Adjacent the lower surface of the sponge and in electrical contact therewith is an elongated platinum electrode 69 which by the lead 71 is adapted to complete the electrophoretic circuit within said chamber. The buffer moistened sponge 65 is partly immersed within the buffer solution 29. One example of a buffer solution is: Barbital 1.84 gm, Sodium Barbital 10.30 gm dissolved in one liter of water.
Plate holder rack 73, FIGS. 1, 2, 3 and 4 is constructed of MYLAR (Trademark) or other non-conductive plastic material and includes an elongated horizontal rectangular open frame 75 which at one end terminates in the upright support arm 77 which at its upper end terminates in the top plate 79. Said plate projects through notch 41 in the end wall 39 of container 25 and includes at its end an aperture 81 to receive the fastener 140 for securing the plate holder rack to the block 139 connected to the lift and transfer mechanism hereafter described. One or a series of parallel spaced rectangular sample plates 83 are arranged in an upright position, bowed or flexed as shown, FIG. 4 and frictionally retained within hollow frame 75 forming a part of the plate holder rack.
Each of the sample plates 83 are electrically non-conductive, being made of a plastic material and having a MYLAR (Trademark) backing, for illustration. Upon the opposite side of the plate is an electrophoresis media such as cellulose acetate. The electrophoresis media may be of other materials (agarose, cellulose esters, acrylamide gel, etc) which are known to be electrically conductive for use in the electrophoresis method, such as disclosed in U.S. Pat. No. 3,808,118 of Apr. 30, 1974 and issued to applicant's assignee, Helena Laboratories Corporation, Beaumont, Tex.
The present lift and transfer assembly, FIGS. 1, 2 and 3 includes an electric motor 87, fragmentarily shown in FIG. 1, connected to the speed reducer 89, both mounted upon base 13, having an output shaft 91 mounting pulley 93 which by the belt 95 drives pulley 97 secured upon the elongated horizontal feed screw 99.
A pair of longitudinally spaced opposed journal blocks 101 are mounted and secured upon brackets 103, FIG. 1 and is secured by fasteners 105 to base 13. The horizontal feed screw 99 is supportably mounted and journalled upon said blocks and includes end thrust collar 107 in registry with one of the journal blocks.
A pair of horizontally disposed parallel spaced guide rods 109 are arranged upon opposite sides of the horizontal feed screw 99 and at their ends are anchored within corresponding apertures within said blocks.
Horizontally reciprocal feed block 111 has a pair of parallel spaced apertures 113 by which the block slidably receives the guide rods 109 and includes an interior threaded portion 115 in operative engagement with horizontal feed screw 99.
Belt drive motor 117 is supported upon and depends from support plate 119 secured to the undersurface of the feed block 111 and includes an output shaft 120 mounting pulley 122.
Journal block 121 is secured to one end of the horizontal feed block 111 by the anchor bracket 123 and journals and supports the vertical feed screw 125 having a thrust washer 127 in engagement with journal block 121 in order to maintain the upright position shown in FIG. 2.
Belt 129 interconnects pulley 122 and the pulley 133 upon the end of the vertical feed screw 125.
Vertical feed screw 125 receives and is threaded at 137 into the vertical feed block 135 upon which is mounted and secured the transverse plate holder support 139 which mounts and supports the plate holder rack 73 as shown in FIG. 2.
A pair of upright parallel spaced guide rods 141 loosely and guidably extend through the vertical feed block 135 and at their lower ends project into corresponding apertures within and are anchored upon journal block 121.
The upper journal block 121, FIGS. 1 and 2 is apertured to receive and be secured to the upper ends of the corresponding guide rods 141 and also journals the upper end of the vertical speed screw 125.
The mounting of the guide rods 141 and the corresponding vertical feed screw 125 upon the single journal block 121 is sufficient to maintain the members 141 and 125 in the upright position shown as movably mounted and supported upon the horizontal feed block 111. As shown in FIGS. 2 and 3, the down limit switch 145 is adjustably mounted upon and secured to one of the guide rods 141 in the path of downward movement of the vertically adjustable feed block 135 and is connected into the circuit with the motor 117 so as to deenergize the motor when the block 135 has reached the position shown in FIG. 2.
An up limit switch 147 is adjustably secured upon the other of the pair of upright guide rods 141. The limit switch 147 is in the path of upward movement of the vertical feed block 135 and is adapted to again de-energize the vertical feed motor 117, when it has reached its uppermost position as preset by the location of the uplimit switch 147. This automatically deactivates the vertical feed motor 117.
It is contemplated that the engagement of the upper limit switch 147 by the vertical feed block 135 could additionally energize the horizontal feed motor 87 to provide a horizontal intermittent feed to the horizontal feed block 111 with respect to the power rotated horizontal feed screw 99.
Motor 87 and the output shaft 91 from the connected speed reducer 89 will rotate causing continuous feed movement of the horizontal feed block 111 until the plate carrying rack 73 has been centralized with respect to the underlying vats successively 45, 47, 49, 51, 53 and 55 in a vertical stepping motion. Suitable stop means are thereby included for de-energizing the horizontal feed motor 87 at the appropriate period so that the plate carrying rack is in a central position overlying one of the said vats. Thereafter the vertical feed motor 117 is activated so as to cause a downward movement of the vertical feed block 135 lowering the rack and mounted plates 83 into the respective vats for immersion within the solutions therein.
The plate carrying rack 73 at all times maintains the plates 83 in an upright position so that after the initial electrophoresis process within container 25, the plates are lifted in a vertical plane by the lift and transfer assembly successively moved forward and thence downwardly into the next succeeding vat, such as vat 45 for immersion within the stain fluid therein.
After the initial electrophoresis process for a predetermined set interval, initial activation of the power operated lift and transfer unit and the energizing of the vertical feed motor 117 causes an upward movement of rack 73, mechanically disengaging the cover 31 from the resilient retractable hold down clips 35 with the cover opening to the dash line position shown in FIG. 1.
The movements of the plate carrying rack 73 which successively transfers the plates carried thereby from the electrophoresis chamber and for immersion successively and intermittently into the respective solution containing vats 45 through 55 as a linear stepping motion with the plates in the first instance transported from the electrophoresis chamber 27 into the fluid within the stain vat 45 for immersion therein for a predetermined interval. On successive lift and transfer movements, the stained plates are successively lifted, advanced and lowered into the respective solutions within the additional vats 47, 49, 51, 53 and 55.
The invention is further directed to the method of electrophoresis which includes the following steps:
1. Supporting in an upright position, a non-conductive plate 83 having on one surface an electrophoresis media to which has been applied a sample selected from the group consisting of serum proteins, lipoproteins, hemoglobins and isoenzymes, for example. The supporting of the plate or a plurality of such plates, employs a plate holder rack 73, FIG. 2, which includes the horizontally open frame 75 within which the plates are bowed and frictionally retained in the upright position shown. The plates 83 are supported for suspension within the electrophoresis chamber 27, FIG. 1.
2. A further step, includes the enclosing of said plate or plates within the chamber 27 by closing the cover 31 and thereafter applying an electrical potential to the plate ends within an electrical power circuit for a preselected period, at a preselected voltage, wherein the specimen elements fractionate and migrate laterally through portions of the electrophoresis media 85.
In the illustrative embodiment, the membrane is a cellulose acetate, but it could be made of other electrophoresis media, such as agarose, cellulose esters, acrylamide gel and other types for plate electrophoresis.
In the present method, the electrophoresis chamber 27 has a hinged cover 31 wherein the application of electrical potential includes the spaced electrical conductors 61 and 69 on the bottom of container 25 and upon cover 31.
3. The method includes the positioning of a buffer moistened conductive sponge 65 within a buffer solution 29, within the container to overlie the conductor 69 and thereafter the positioning of a second buffer moistened conductive sponge 57 depending from the cover in engagement with the second conductor 61. The method includes the positioning of the top and bottom edges of the plates 83 so as to be yieldably embedded into the surfaces of the respective sponges for completing the electrical circuit therethrough when the cover 31 is in the closed position shown in FIGS. 1 and 2.
The present method includes the use of a plate supporting and transfer rack 73 having an open framework 75 with the plates 83 horizontally bowed for frictional retention therein.
4. The present method includes the further step of elevating the plate or plates 83 after the electrophoresis process and translating the plates while maintaining them in an upright position and thereafter lowering the plates and immersing them within a stain solution of vat 45 for a predetermined interval.
5. The present method also includes in connection with the use of a power operated lift and transfer assembly 135, a means by which the plate holder rack 73 and the mounted plates 83 are successively transferred and immersed within their respective solutions in vats 45, 57, 49, 51, 53 and 55, for a predetermined period in a continuous linear stepping motion.
For the electrophoresis process, the technician pours a small amount of buffer solution 29, FIG. 1, into the bottom of chamber 27, being careful not to cover sponge 65. He then places the sample holder 73 with the sample plates 83 into the chamber 27. Rack 73 has a support 79 converted to the left and transfer unit. The plates 83 will push gently into the sponge 65 and will thus come into contact with the buffer solution therein. An additional electrode 61 is placed in contact with sponge 57. When the lid 31 is in the down position, its depending contact is in registry with a contact at the top of the chamber, which is electrically connected to conductor 69.
These six vats, preferably of plastic, are aligned with the electrophoresis chamber 27 upon the platform 23 so that the plates 83 can be immersed into each thereof in their proper order, i.e., dye for vat 45, rinse solution number 1 for vat 47, rinse solution number 2 for vat 49, rinse solution number 3 for vat 51, a fixative solution for vat 53 and a rinse for vat 55.
With the vat 73 loaded as in FIG. 4 with the plate or plates 83, vertical feed motor 117 is initially energized lowering the rack to the immersion position within the electrophoresis chamber until the vertical feed block 135 engages the down limit switch 145, FIG. 3.
After a predetermined preset interval including a timer within an electrical circuit to a power source, the vertical lift motor 117 is reactivated elevating the vertical feed block 135 and the rack 73, until it engages the uplimit switch 147 de-energizing the motor 117. At that time the horizontal feed motor 87 is automatically energized for a period until the loaded rack is transfered from the elevated position corresponding to FIG. 2 until it centrally overlies the first vat 45 and is automatically de-energized. In the electrical circuit the vertical feed motor 117 is energized so that its output shaft operates in the reverse direction rotating the vertical feed screw 125 and accordingly lowering the vertical feed block 135 and the connected rack and plate so as to be immersed within the solution in stain vat 45.
In accordance with a preset time, after a period of immersion, the vertical feed motor 117 is reactivated for drive in the opposite direction elevating the rack 73 to its uppermost position until the block 135 engages an uplimit switch 147. At that time, the horizontal feed motor is again energized for a period such as would feed the loaded rack to the next succeeding vat 47 centrally thereof, etc.
By this process, the loaded rack is advanced horizontally by the horizontal feed screw with interrupted longitudinal feed movements so that the rack centrally overlies the respective vats successively and is automatically lowered into the fluid therein and immersed for a predetermined period of time, and thereafter retracted and horizontally advanced to the next succeeding vat. Thus the loaded rack has been moved longitudinally with respect to the respective vats and has at each vat been lowered and immersed within the solution therein for a predetermined interval and thereafter elevated. The foregoing movements may be defined as a linear stepping motion.
In the illustrative embodiment, a stain referred to as "PONCEAU S" is employed within the stain vat 45 in a solution which is filled into the vat 45 to within 10 MM of the top to assure complete staining of the membranes.
The first rinsing compartment 47, for example, contains a solution of 5% GLACIAL acetatic acid. The second rinsing compartment 49 also contains a 5% GLACIAL acetatic acid. The third rinsing compartment 51 also contains the 5% GLACIAL acetatic acid.
The fixing vat 53 contains a methanol solution. The methanol dehydrates the plate or plates and removes the excessive water. The methanol should be changed daily or after dehydrating 10 plates. A contaminated methanol will create a cloudy background on a finished plate.
The clearing vat 55 contains a clearing solution which is composed of:
67 parts Methanol
28 parts Glacial Acetatic Acid
4 parts Clear Aid Helena (Catalogue No. 5005)
The clearing solution should be changed regularly to insure proper clearing of the cellulosic acetate. The clearing solution is used to soften the cellulose acetate membrane or other membrane employed.
The electrophoresis is employed in the chamber 27 using the same technique as would be employed for serum protein. To stain the lipoprotein plate, the operator must substitute for the Ponceau S in the chamber 45 with one filled with Oil Red Om and 1.0 normal sodium hydroxide.
330 mL Oil Red Om
110 mL 1.0 Normal Sodium Hydroxide
The lipoprotein plate can stain for a recommended time of 1 hour approximately.
This requires an offset application. Migration will be from the negative to the positive. Thus, the polarity switch, not shown, should be switched to positive. Staining, destaining and clearing of hemoglobins is identical to the serum protein format. Lipoproteins should be run at 180 volts for 25 minutes. The hemoglobins should be performed at 350 volts for 25 minutes.
Having described my invention, reference should now be had to the following claims.
|Cited Patent
|Filing date
|Publication date
|Aug 16, 1976
|Nov 22, 1977
|Beckman Instruments, Inc.
|Automated electrophoresis unit
|Jun 21, 1976
|Nov 7, 1978
|Harald Dahms
|Method and apparatus for electrophoresis
|Citing Patent
|Filing date
|Publication date
|Jan 30, 1986
|Apr 14, 1987
|Fotodyne, Inc.
|Foto/phoresis apparatus
|Jun 27, 1988
|Dec 26, 1989
|Helena Laboratories Corporation
|Automatic electrophoresis apparatus and method
|Jan 2, 1990
|Sep 10, 1991
|Pietro Nardo
|Unit for the treatment of electrophoretic strips
|Jul 7, 1993
|Mar 21, 1995
|Helena Laboratories Corporation
|Platform for conducting electrophoresis, and electrophoresis plate for use with the platform
|Sep 21, 1993
|Oct 24, 1995
|Helena Laboratories Corporation
|Automatic electrophoresis method and apparatus
|Dec 22, 1994
|Apr 30, 1996
|Helena Laboratories Corporation
|Electrophoresis plate
|Jun 7, 1995
|May 14, 1996
|Helena Laboratories Corporation
|Automatic electrophoresis method and apparatus
|Nov 21, 1994
|Dec 3, 1996
|Helena Laboratories Corporation
|Support system for an equipment housing
|Jun 7, 1995
|Dec 10, 1996
|Helena Laboratories Corporation
|Method for calibrating a piston and cylinder type applicator
|Dec 27, 1994
|Jun 10, 1997
|Helena Laboratories Corporation
|Platform for conducting electrophoresis, and electrophoresis plate for use with the platform
|Apr 25, 1997
|Dec 8, 1998
|Helena Laboratories Corporation
|Automatic electrophoresis method and apparatus
|May 20, 1997
|Feb 2, 1999
|Academy Of Applied Science
|Automatic protein and/or DNA analysis system and method
|Sep 10, 1998
|Mar 14, 2000
|Academy Of Applied Science
|Automatic protein and/or DNA analysis system and method
|May 1, 1997
|May 30, 2000
|Helena Laboratories Corporation
|Automatic electrophoresis apparatus with fluorescent and visible scanning
|Jun 22, 1998
|May 30, 2000
|Koh; Francis H.
|Multi-tank gel developing apparatus and method
|Aug 26, 2003
|Aug 28, 2007
|Helena Laboratories
|Automatic in situ electrophoresis method and apparatus
|Aug 3, 2004
|Feb 9, 2006
|Gustavo Garcia
|Transilluminator having light emitting diode (LED) array
|Mar 15, 1988
|Nov 6, 1997
|Helena Lab Corp
|Verfahren zur Auswertung elektrophoretisch behandelter Probentr�ger
|Mar 15, 1988
|Jul 24, 1997
|Helena Lab Corp
|Automatic electrophoresis analysis appts.
|Oct 27, 1989
|May 3, 1990
|Helena Lab Corp
|Appts. for automatic electrophoresis - having lower section, mounting plate, transverse troughs in support, scan unit, computer and crane arrangement
|Dec 1, 1989
|Aug 2, 1990
|Helena Lab Corp
|Automatic electrophoresis unit - combines pye automatic performance of all consecutive operational stages
|Nov 13, 1992
|May 19, 1993
|Olympus Optical Co
|Automatic electrophoresis unit - includes unit for supplying substrate sheets to buffer wetting unit, or feed unit with controlled shear and feed units, or several feed units for supplying sheets of different length
|May 24, 1984
|Mar 20, 1985
|Georgetown University
|Apparatus and method for separating polynucleotides and detecting specific polynucleotide sequences
|Sep 7, 1999
|Mar 16, 2000
|Biocrystal Limited
|Device and process for automated cell staining
|U.S. Classification
|204/608, 204/613, 204/618, 204/641
|International Classification
|Cooperative Classification
|European Classification
|Jun 12, 1981
Owner name: HELENA LABORATORIES CORPORATION, 1530 LINDBERGH DR
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:GOLIAS, TIPTON L.;REEL/FRAME:003894/0575
Effective date: 19810601
|May 27, 1986
|Fee payment
Year of fee payment: 4
|May 22, 1990
|Fee payment
Year of fee payment: 8
|May 20, 1994
|Fee payment
Year of fee payment: 12


text:
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subset: commoncrawl


text:
Constance "Connie" Booth (born 31 January 1944) is an American-born writer, actress, comedian and psychotherapist based in England, best known for appearances on British television and particularly for her portrayal of Polly Sherman in the ...popular 1970s television show Fawlty Towers, which she co-wrote with her then-husband John Cleese.
Connect any celebrity with Connie Booth to see how closely they are linked... romantically!
I don`t know what Connie Booth looks like now, but in Fawlty Towers, she was one of the most beautiful women I had seen ever.How can we get current pics?
Great actress and so attractive


text:
WASHINGTON — In a symbolic show of support for Ukraine's fledgling government, President Barack Obama is meeting with Ukrainian President Petro Poroshenko in the Oval Office on Thursday after the leader of the former Soviet republic speaks to a rare joint session of Congress. Continue reading
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Russian President Vladimir Putin has evoked Ukraine's Soviet past as a way of justifying the annexation of Crimea, and to appeal to many Ukrainians. Putin has argued that post-Cold War expansion of NATO has provoked Moscow. Chief foreign correspondent Margaret Warner talks to Andrey Kozyrev, Russia's first foreign minister after the fall of the Soviet Union, for insight into the conflict. Continue reading
The Associated Press and other news outlets reported heavy gunfire and explosions around the eastern city of Slovyansk, where Kiev's army mounted its first major offensive Friday against armed pro-Russian insurgents. Continue reading
WASHINGTON — NATO's second-ranking official says Moscow's annexation of the Crimean Peninsula and its destabilizing actions in eastern Ukraine have compelled the U.S.-led alliance to start treating Russia as more of an enemy than a partner. Continue reading
The new penalties were a response to what the West says is Russia's failure to live up to commitments it agreed to under an international accord aimed at ending the dispute. The White House says Russia's involvement in the recent violence in eastern Ukraine is indisputable and warned that the U.S. and its partners were prepared to impose deeper penalties if Russia's provocations continue. Continue reading


text:
Programming with the XForms Library
Where did Linux come from? That's a good question, and one that seems to confuse a lot of new users. The short answer, of course, is that Linux was written by Linus Torvalds (the original and central force behind the Linux kernel). But that cursory response misses much of the spirit of Linux. When you go into a computer store and buy a Linux CD-ROM set, you're getting a copy of the intellectual output of literally thousands of programmers. Most of these people have never met each other. Thanks to the Internet, however, this geographically dispersed group has managed to create a formidable array of tools and applications. Linux works so well that it creates the illusion that there is some kind of organization and control structure in place—some central authority directing every aspect of its development. This makes it easy to forget that every application you run—from an ASCII text editor to a Z-Modem download—exists because somebody, somewhere, sat down one day and said, "I can't find a way to do this under Linux, so maybe I should try to write my own program."
This article is the first in a three-part series designed to introduce the reader to a programming tool called The XForms Library. As its name suggests, XForms is a set of tools and routines that can be used to easily create programs that run under the X Window System. The overall goal is to take a reader who has never written an X-based piece of software to the point where he or she can start writing their own applications and utilities. The series assumes the reader is familiar with the C programming language, since XForms is C-based. If you don't yet know anything about C programming, now may be a good time to take the plunge. Buy a book or two and start fiddling with it. With a few weeks of study, you'll probably have enough C under your belt to be able to follow the series by the time the next article appears. It is also assumed you have a working C compiler and the X Window System is already installed.
It's the author's hope that readers will eventually be able to contribute an XForms-based program to Linux. The next time you post a "where can I find a program to do x" message to comp.os.linux.misc and don't get an answer, think about whether it's something you might be able to write yourself. You probably won't earn a cent for your troubles, but the process is not without its rewards. One day, a few months after uploading your package to Sunsite, you may find yourself in a software shop that has just received a new shipment of Linux CD-ROMs. Up on a shelf, probably squeezed between the latest commercial software from Microsoft and Corel, will be a few kilobytes of code you wrote and which people all over the world are using.
The purpose of this first article is to explain how to get and install XForms. We also take a first glance at the process of creating an XForms application and actually write a couple of simple programs.
The next two articles, which will appear in the August and September issues of Linux Journal, will expand on this process. They will go through all the steps required to create useful software with XForms with the aid of an example application, a simple game theory simulator. Don't worry if you have no idea what game theory is, since all you need to know about it will be explained in the process. The choice of programming example is driven by the author's interests, and is irrelevant to the central purpose of the series: learning to use XForms.
Details on where to get all of the software mentioned are available in the Resources section at the end of this article. There is also a web page for this series which can be found at http://a42.com/~thor/xforms/. This site includes links to all relevant software, as well as listings of all the source code used in the series.
The XForms library was written by T. C. Zhao and Mark Overmars. It is free for non-commercial use, but if you're planning on selling your application, you will need to set up a monetary licensing arrangement with the XForms authors. If you're a free software "purist", you may want to think about using V instead of XForms (see the next section).
The most important parts of the XForms package are the actual library (libforms) and the forms.h include file. The former contains functions for creating buttons, menus, and so on. The include file declares these library functions, which makes them available for use in your software. If you're a relative newcomer to all this, don't worry—we'll be going through every step required to get things working.
There is also a very thorough manual available in HTML format, which explains all of the available routines. A large set of example applications, which are referred to in the documentation, are included in the main distribution. These examples are very useful for getting good ideas on how to implement various procedures.
Finally, there is a program called fdesign included with XForms that can be used to design user interfaces. This is a real time saver, since you can use your mouse to control the placement of buttons, menus and other objects. Your design can then be saved to a file and easily brought into your source code. Complicated sets of overlapping windows with large numbers of graphical elements can thus be created in a relatively short period of time.
Since XForms is actually built around the X11 library, any program you write is highly portable to other X-capable systems. Although readers of this magazine will probably be developing for Linux, it's nice to know users of FreeBSD, Sun and other systems will be able to run your application if they want.
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This ebook takes a look at some of the practical applications of the Linux on Power platform and ways you might bring all the performance power of this open architecture to bear for your organization. There are no smoke and mirrors here—just hard, cold, empirical evidence provided by independent sources. I also consider some innovative ways Linux on Power will be used in the future.Get the Guide


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MOSCOW President Vladimir Putin has ordered Russian authorities to protect state computers from hacking attacks, the Kremlin said on Monday, after an Internet security firm said a spy network had infiltrated government and embassy computers across the former Soviet bloc.
Dubbed Red October, the network used phishing attacks - or unsolicited emails to intended targets - to infect the computers of embassies and other state institutions with a programme designed to harvest intelligence and send it back to a server.
Putin signed a decree on January 15 empowering the Federal Security Service (FSB) to "create a state system for the detection, prevention and liquidation of the effects of computer attacks on the information resources of the Russian Federation".
State computer and telecommunications networks protected by the cyber security system should include those inside Russia and at its embassies and consulates abroad, according to the decree, which was published on a Kremlin website on Monday.
The Russian Internet security firm Kaspersky Labs said last week that the computer espionage network, discovered last October, had been seeking intelligence from Eastern European and ex-Soviet states including Russia since 2007. (r.reuters.com/mag45t)
Many of the systems infected belonged to diplomatic missions, Vitaly Kamluk, an expert in computer viruses at Kaspersky Labs, said last week. He declined to name specific countries.
Kamluk said last week that the network was still active, and that law enforcement agencies in several European countries were investigating it.
Kaspersky Labs said the infiltrators had created more than 60 domain names, mostly in Russia and Germany, that worked as proxies to hide the location of their real server.
The FSB declined immediate comment last week when asked whether Russia had taken action to bring any suspected members of the espionage network to justice, or acted to improve Internet security in light of the discovery.
The FSB - the main successor agency of the Soviet KGB - requested a written query, to which it has not yet responded. The Kremlin declined immediate comment on Monday when asked whether Putin's decree was linked to Red October.
(Reporting by Steve Gutterman and Thomas Grove; Editing by Kevin Liffey)
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The Wonders of the Cosmos presentation was a trippy, far-out exploration of the Jet Propulsion Laboratory's achievements and triumphs on earth as well as into deep space and beyond. Hosted by the Chevy Chase Estates Association's very own Leigh Torgerson, an engineer with JPL, the afternoon began with a discussion and photo archive of the post WWII US rocket program and ended with a peek into planned future explorations to asteroids, planets, and distant solar systems. Also contributing to the discussion was another CCEA director and JPL cohort, Douglas Hughes. This program was made possible by JPL, and your neighbor's membership support of our canyon CCEA. Click for event photos.
Join us Saturday May 21st from 2-4 pm at the Chevy Chase Library for a special presentation of JPL: Yesterday, Today and Tomorrow. Learn about the many discoveries, vistas and wonders of our universe made possible by the Jet Propulsion Laboratory, JPL history as well as future projects. Featured speakers will be our own JPL Canyon residents and will include out of this world slides and video. A treat for the whole family! Check out the special Bonus below!
Special Bonus!
Enjoy the amazing video Journey to the Planets and Beyond narrated by Harrison Ford.
Click here or on the photo above to see a slideshow from the evening.
The 79th Annual Meeting of the Chevy Chase Estates Association was a huge success attended by your friends and canyon neighbors, along with stars of local Glendale government including Council Members Laura Friedman and Vartan Gharpetian, our Mayor Ara Najarian, and Chief of Police Robert Castro. The evening's featured event was a detailed and informative report on GPD's new Community Impact Bureau, a proactive special enforcement and crime analyst unit tasked with fighting the increase of crime due to easing of criminal laws. Thanks to Lt. Oscar Rodriguez, Officer James Colman, Abby Luczon, all of the Glendale Police Department for the great presentation. And a special thanks to Tom Lorenz for making so much of this possible.
Click to download an official tip sheet of the GDP terminology definitions used in the presentation, and the difference between burglary and theft as delineated in the CA Penal Code.
The 79th Annual Meeting of the Chevy Chase Estates Association will be held on Wednesday, March 9, 2016, at Chevy Chase Library, 3301 East Chevy Chase Drive. The evening will start with a half-hour social at 7:00 p.m. At 7:30 p.m., we will conduct the business portion of the meeting, including the President's report, Treasurer's report and election of Directors.
Renew CCEA memberships and / or purchase the new Chevy Chase History Book donate here!
Police Lieutenant to Address Residents
Crime is up, and even our peaceful canyon isn't immune to the negative effects. To keep you updated on this important topic, Lieutenant Oscar Rodriguez of Glendale Police's Community Impact Bureau will be the guest speaker at this year's Annual Meeting. Lt. Rodriguez will discuss public safety for all Glendale residents, with particular emphasis on issues which affect the Canyon. Don't miss this important opportunity to hear directly from one of the people most responsible for keeping Glendale—and you—a safe community.
A wonderful collection of early Glendale photos has been assembled in a blog and can be seen by clicking on the photo below!
The Kett Residence located in the Chevy Chase Canyon of Glendale, California was designed by A. Quincy Jones and completed in 1952. Full sized photo click here.
Is your home here? Can you find your neighbor's?
Click to see a photo slideshow this year's Canyon Christmas Light Displays.
Homes were photographed at random so if your house isn't included, perhaps it can be found in the lights of years past below. Enjoy these beautiful canyon treasures, Merry Christmases (Glendale is so cool, we celebrate two, the 25th and the 6th.), Happy Holidays, and a very Happy New Year from your neighbors at the CCEA.
Click here to find a 2014 Christmas home.
Click here for the 2013 homes.
Say goodbye to the old Chevy Chase Country Club clubhouse. As the jewel at the heart of our community, in one form or another it has served the canyon since the late 1920's. But fret naught, from the rubble will soon rise a new spectacular clubhouse. A photo slideshow of the demolition work can be seen here.
Click on the photo above to see more photos.
Artist rendering of the new clubhouse.
From left to right; William McKinley, Brit Trydal, Joan Binzley, Frances Muir, Mary Betlach, Orchid Donnelly.


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Dracula Has Risen From the Grave
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Dracula Has Risen from the Grave (1969) has Christopher Lee reprising his role as the ultimate blood-sucking creature of the night, which is kind of strange as in the last film, Dracula, Prince of Darkness (1966), he was destroyed...or so we thought. The film takes place a year after the last film, as Monsignor Ernest Muller (Rupert Davies) visits the small village near Dracula's no empty castle to see how things are getting along. Well, things aren't getting along too well as the Monsignor finds the church empty and in a rather poor state of housekeeping. Finding the priest at the local bar, he learns that the villagers believe that while Dracula may be dead, his castle projects an aura of evil, casting a malignant shadow of evil on the town. The Monsignor decides the only course of action is to trek up to the castle, say a prayer of exorcism, and place a fairly large cross at the doorway, expelling the curse forever. Taking the priest with him, the two begin a long and arduous journey up the mountain, but, as they near the castle, the priest balks from fear, and the Monsignor continues on alone. He reaches the castle, says the prayer, and places the cross (the result of both actions cause a rather freakish lighting storm...good thing he rid himself of that big, metal cross). Meanwhile, the priest, who remained behind, starts freaking out, and begins to stumble down the mountain, trips, cracks his head open, and lands on and cracks a pool of ice, one which contains the body of Dracula, and begins to bleed onto the ice, to which the blood revives the dark, yet frozen, one.Read more ›
The film opens in a small, pre-industrial village that had previously been terrorized by Dracula, who is presumed to have been destroyed. As the title of the film indicates, however, Dracula soon rises from his grave to begin a new campaign of bloodsucking villainy. His foes in this film are a Catholic monsignor and the boyfriend of the monsignor's lovely niece.
Bernard's solid score is complemented by good art direction. Francis makes effective use of forest and rooftop settings as Dracula pursues his ends. Lee gets solid support from the rest of the cast. Barry Andrews makes a particularly appealing young hero as Paul, the boyfriend of the monsignor's niece. I also was impressed by Barbara Ewing's performance as Paul's sexy co-worker.
There is a pronounced sexual feel to vampirism in this film (as in the other Lee Dracula films I have seen), so there is a delicious irony to the fact that one of Dracula's key foes here is a Catholic monsignor, and thus presumably celibate. Also, a religous controversy among Dracula's foes makes for an interesting contrast to their battle against the undead villain. Overall, a well made and solidly entertaining film.
Ably directed by veteran Hammer man Freddie Francis the film opens a period of time after the conclusion of the earlier film where a small village experiences a horrible death in the bell tower of the local church. The terrified townspeople still living in fear from the shadows cast by Dracula's abandoned castle prompt a visiting Monseignor to travel up to Castle Dracula with the cowardly local priest to ensure that the vampire curse is removed once and for all by placing a holy cross over the main entrance door. Unknowingly the priest awakens Dracula with some of his own blood and soon the world's most famous vampire is alive and seeking revenge on the Monseigner and his family, in particular his pretty neice who becomes the object of his attentions.
Apart from the superb "Horror Of Dracula" that immortalised Christopher Lee in the role of Dracula this is the finest entry in the series. Extremely atmospheric with a clever use of day/night photography, great period detail, eerie forest and cemetery scenes and hazy rooftop shots the film is a superb period horror tale.Read more ›
I still don't understand what is so exceptional with "Horror of Dracula" or "Dracula Prince of Darkness"? Maybe it is just name brand awarness gone amuck for some reviewers? I do prefer "Prince of Darkness" over "Horror of Dracula" but "Risen From the Grave" has it all for my money.
This film is a must! The picture is perfect, the acting first rate, the music truly haunting.
**** Hollywood directors/producers take note, if the Hammer production company could make a film like this for $150.000 in 1968 why can't you folks with your infinite wisdom make a decent film with today's 50 million dollar plus budgets?????
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A sequel to Dracula Prince of Darkness.The film takes off where the previous one ended where the blood sucker found his doom under the icy waters of his castle's moat river. Read morePublished 1 day ago by Zebra3
Who killed the girl found in the large church bell in the beginning of the film? Dracula was still dead and frozen at this time.Published 1 month ago by para diddle
Old classic! Fun to watch in those rainy days or with popcorn on a Saturday night!Published 2 months ago by Lorraine B.
These old movies are still among many favorites from a younger age and an earlier era and they "should NOT be having a damn rental price on them and or a BUY ONLY... Read morePublished 2 months ago by Amazon Customer
I love this movie and saw this when it came out and was the first horror film that i had watched in the Theater. Read morePublished 2 months ago by AlphaPrime76
Loved these old horror classics as a child. Watched "Chiller Theater" with Bill Cardill in Pittsburgh PA every Saturday in the 1970s so this was a very nostalgic purchase. Read morePublished 2 months ago by VickyC
Dracula Has Risen From the Grave was the 3rd Hammer film to feature Christopher Lee as Dracula. It's an improvement on Prince of Darkness that came before it. Read morePublished 3 months ago by Alan C. Logan
Classic Hammer horror feature... just the music and opening credits at the beginning of the movie scared me as a kid at the local theatre.. Read morePublished 5 months ago by Flying4905
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I notice your December 2000 cover shows an IT guy as a fat, balding, bespectacled, bearded geek in an XXL black T-shirt and with an attitude problem. I'm most insulted by this stereotype. I shave.
—Bruce Richardson email@example.com
Our cover model, Glenn, responded to an open ad to system administrators and was chosen for his expressive qualities.
I recently received the December 2000 issue of Linux Journal and went right to one of your feature stories—"A Linux-Based Automatic Backup System". I was expecting to see something about tape drives and backing up and restoring a dead hard drive. Instead, I see a very brief tutorial on using Samba, cp, cron, and bzip on backing up Windows shares on a Linux hard drive. The author quotes that it's an "inexpensive and easy solution"—he's exactly right about that. I don't see creating a zip file on a hard drive a backup solution, nor do I see this article being a feature story. There was absolutely nothing in the article that even mentioned backing up Linux files or how to restore them. Articles like this and the wave of many others that you have published in the past are a complete embarrassment to Linux. They all seem to be Linux acting in a supporting role to Windows. Come on! Look at the front cover of your magazine! Publish some real articles about Linux for a change!
—Paul Sullivan email@example.com
Thank you for your short article in the December 2000 issue of LJ on the trouble with the Bastard Operator from Hell. Interesting from my point of view that I've never felt that my job has sucked. It's often busy and I have had to deal with a lot of bozos, but I've generally been having a good time. And I'm somewhat in the minority in that I did major in computer science.
I do have one question, though. I've just recently (this week) started at a new employer. Problem is, I've run into someone in my group who likely doesn't think BOFH is funny at all. Rather, he thinks it's a manual on how to be a systems administrator. I kid you not. So what do you do when you encounter a real life BOFH? It's serious enough that I think the guy should be just outright fired. I still don't know how he got hired and how he's lasted as long as he has.
Unfortunately, there's no Getting People Fired HOWTO. However, Simon Travaglia's BOFH stories, The Art of War by Sun Tsu and The Prince by Nicolo Machiavelli are all available on-line.
In Reuven Lerner's "At the Forge" article in the December 2000 issue he states "...CGI has a number of drawbacks...,it requires that the web server spawn a new process." It saddens me to see that a regular columnist at Linux Journal misunderstands the meaning of CGI.
Even though he defines CGI as "common gateway interface" he fails to continue with the thought. It is an interface to a server. It is not a program. Any process that processes data given to a server with a QUERY_STRING is a "CGI program". That means that mod_perl scripts, PHP, servlets, JSP, etc., all of which run in the server memory space—thereby not spawning new processes—are also CGI programs.
Yes, the common misconception is that CGI is a type of scripting, but as the leaders of the industry we should be precise in our language and meaning.
—Nathan Hokanson email@example.com
I enjoyed the article by Frode Tennebo on Ada 95 programming in the December 2000 issue. It's exciting to see Ada's increasing popularity in the Linux world. It's because of the language's features and robustness that best-selling Linux author Warren W. Gay announced that he would be porting his open-source projects to Ada.
For the benefit of your readers, more information about Ada Linux programming is available through the Big Online Book of Linux Ada Programming (www.vaxxine.com/pegasoft/homes/book.html). This is one of the largest on-line resources for Linux programming for any language and is a good starting point for newbies.
Thanks for letting your readers know that there are alternatives out there to C++ and Java.
—Ken O. Burtch email@example.com
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Cron traditionally has been considered another such a tool for job scheduling, but is it enough? This webinar considers that very question. The first part builds on a previous Geek Guide, Beyond Cron, and briefly describes how to know when it might be time to consider upgrading your job scheduling infrastructure. The second part presents an actual planning and implementation framework.
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|Jul 18, 2016
- The Firebird Project's Firebird Relational Database
- Stunnel Security for Oracle
- My +1 Sword of Productivity
- Non-Linux FOSS: Caffeine!
- Managing Linux Using Puppet
- SUSE LLC's SUSE Manager
- Murat Yener and Onur Dundar's Expert Android Studio (Wrox)
- Doing for User Space What We Did for Kernel Space
- SuperTuxKart 0.9.2 Released
- Google's SwiftShader Released
Geek Guides
With all the industry talk about the benefits of Linux on Power and all the performance advantages offered by its open architecture, you may be considering a move in that direction. If you are thinking about analytics, big data and cloud computing, you would be right to evaluate Power. The idea of using commodity x86 hardware and replacing it every three years is an outdated cost model. It doesn't consider the total cost of ownership, and it doesn't consider the advantage of real processing power, high-availability and multithreading like a demon.
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Where in mass are you located?
Type: Posts; User: manchvegasmk11
Where in mass are you located?
The smoking hare looks sick Tom. Can't wait to see it in person at wolfsgart
So their's no where in NH that has an rc drift tack track.
I solved that problem by putting one in my yard.
I was messing around tonight and decided to color match a set of bbs.
Here is my 1:1 that is...
New shell I'm working on.
iI'I'm building a replica of my 1:1 mk1. The single round setup is temporary.
My rc collection and shells.
Hpi crawler king with jeep Cherokee and ford bronco bodies.
Hpi sprint 2 drift with 68 camaro, volksrod, ae86 and skyline r34 gtr bodies.
My sprint chassis is fully...
Heres my hpi sprint 2 drift with hpi toyota ae86 levin body.
Upgrades/mods ive done:
locked rear differential
hpi front one way differential
yeah racing 38mm steel shafts- front and rear...
Heres the parts
Prothane adjustable bump stops. Looking to get $30 shipped.
Im pretty sure ill be there
As stated. Anything aftermarket is illegal in NH.
most local cops dont give a sh*t, its the state troppers that will bust your balls.
Go see Sam
Sams euro shop.
3 tallwood drive unit 1
Bow NH
Whats it got for seats?
Van is looking good.
I'd like to get my hands on a fridolin


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Candlewood Suites Port Royal hotels are listed below. Search for cheap and discount Candlewood Suites hotel rooms in Port Royal, VA for your group or personal travels. We list the best Candlewood Suites Port Royal hotels & motels so you can review the Port Royal Candlewood Suites hotel list below to find the perfect place.
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Enter your stay dates and any discounts you qualify for to see Port Royal, VA rates found only on HotelPlanner!
How should we direct your call?


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Hearts manager John McGlynn has suggested some referees go in search of an argument and favours ignoring officials altogether.
St Johnstone boss Steve Lomas was this week handed an eight-match touchline ban, while Celtic boss Neil Lennon, Kilmarnock manager Kenny Shiels and others have been regularly sanctioned by the Scottish Football Association.
McGlynn was twice sent to the stand earlier in his career and vowed to avoid the punishment in future. The former Raith boss said: "I never say a word to the referee. Every game I say to myself: 'don't get involved with the referee'.
"I think they know that as well and they're trying their best, but they'll not win, because I'll never get sent to the stand. I'm winning."
McGlynn used an example from last weekend's 0-0 draw at Motherwell in the Clydesdale Bank Premier League, a fixture during which Darren Randolph and Callum Paterson collided.
Referee Crawford Allan did not take action, but the Motherwell goalkeeper was this week handed a retrospective two-match ban for violent conduct.
McGlynn said: "The referee came and stood right at the dugout waiting for a confrontation. I didn't bother. I try my best not to get involved. I'm representing the football club and I'm trying to show a good aspect of the football club, that I'm not a fruit and nut case.
"The most important things are the players and my close staff. They know how passionate you are."
Speaking ahead of Sunday's William Hill Scottish Cup fourth round clash with Hibernian, McGlynn expressed surprise that Bobby Madden had been appointed to a Hearts fixture for the fifth time this season.
He joked about their relationship, saying Madden, who is to be fourth official at Easter Road, "comes for tea".
- Sports & Recreation
- Soccer


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It would be useful to me to be able to select post types that I want the plugin to monitor (or exclude from monitoring). I have a fancy custom post type that uses permalinks in a non-standard way, and that is confusing Redirection into redirecting from homepage to a post of that post type. Simple exclude of the post type would do the trick. Either option or a filter would be great!
Oh, and thank you for this great plugin!


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Free personalized radio that
plays the music you love
Yeah, Whatever
And you're cynical to me
And I chewed down on the bit
And almost swallowed it
Features of This Trackelectric rock instrumentation
a subtle use of vocal harmony
mild rhythmic syncopation
repetitive melodic phrasing
minor key tonality
electric guitar riffs
These are just a few of the hundreds of attributes cataloged for this track by the Music Genome Project.show more
Similar Tracks
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text:
Car Wrecks – Stats and figures
Why Hiring A Campervan Is The Best Way To Explore Australia
Australia is a huge country and there are so many benefits with hiring a campervan. They are economical, give you complete freedom, are convenient, and are a cost-effective way of seeing all the attractions and landmarks this large continent has to offer.
Just think – no expensive hotel rooms, no early checkouts, no bag packing and complete flexibility to travel whenever suits you. You aren't limited to one destination and if you add up the costs of renting a campervan, you end up saving money that can be spent elsewhere.
This handy infographic gives you an understanding of what a campervan is, the benefits, limitations and some realities you need to be prepared for on your trip:
This infographic was originally published on Flickr
Truck Accidents
Date looking at truck accidents in the US each year.
Current Trends in Freelance Writing
People passionate about writing are fortunate these days as they have more freedom in choosing the type of work they want to pursue. The best part is they can do it in the comfort of their homes. That's the best perk of freelance writing.
In North America today, writers make up nearly half of freelancers. In the U.S., an estimated 53 million Americans are freelancing. Based on the 2012 Freelance Industry Report, 18 percent of freelancers claimed writing as their primary skill while 10 percent said they are skilled in copy-editing and another 10 percent listed copy-writing as their main skill. Other skills listed included translating, web development and marketing.
The number of freelancers is predicted to continue growing moving forward and more companies are expected to embrace this growth. Writers who wish to do freelance work can simply visit several sites including freelance jobs board that provide a list of online and other freelancing jobs. Elance is a popular platform that connects freelancers including writers and editors with contractors looking for staff to outsource certain tasks.
With the trend in content marketing going strong these days, the demand for freelance writers remains high. Reports have noted that companies of all sizes are now allocating a specific budget solely for content marketing and many use freelance writers to meet this demand. In addition, writers can actually more if they are resourceful enough. As of 2010, the median annual wage of writers was at $55,420 or an equivalent of $26.64 per hour based on data from the U.S. Department of Labor.
The outlook for freelance writers is bright. It is predicted to grow six percent until 2010. Among the writers that will be in demand moving forward are those specializing in SEO, social media and content marketing.
Apart from able to work from home, freelance writers can also easily find a job without having to prove their educational background. As long as they can show samples of their writing work, they can eventually get writing projects even from several contractors. Furthermore, as a freelancer, one can choose the niche he or she wants to focus on, set his own rates and create his own service offerings.
An added bonus is that you can actually become a sub-contractor. This means you can create your own team of writers and then accept assignments in bulk. Or you can also accept work from other freelance writers who may need help in writing the many assignments they have.
The infographic below provides more details on the trends in freelance writing.
Cigars Infographic
Via: StogieBoys.com
Walter White's Rap Sheet
An infographic looking at the laws that Walter White of Breaking Bad broke and how much jail time he would theoretically do for each.
Boating Accidents – Stats and Figures
A look at boating accidents statistics
DUI in America
What are the DUI stats in the USA?
Via: Chicago DUI Lawyer
The Evolution of Debt Programs
An infographic looking at debt stats.


text:
Another brick in the whorl
23 February 2012 Feature
The scientists on the inside of advanced fingerprinting research are cross-examined by Simon Hadlington
The oligodeoxyfluorosides before (left) and after (middle) exposure to zinc ions, and the difference between the two (right) © ACS
Having swapped conventional DNA bases for groups whose fluorescence changes in the presence of metals, the chemists' system can use the colours produced as a fingerprint for each metal. 'A single experiment requires an extremely tiny amount of material – only about a picomole of any one chemosensor – so the sensing is quite inexpensive,' Kool tells Chemistry World.
Kool's team previously used its DNA-like oligodeoxyfluorosides to produce sensors to detect toxic gases and food spoilage. Now, they've produced oligodeoxyfluoroside chains from nine building blocks: two simple fluorescent bases, three spacers, plus four bases that both fluoresce and bind metal ions. Varying block choice and position, the chemists created a library containing 6561 four-base chains or tetramers, 174 of which fluoresced strongly when their beads were left in 36 metal salt solutions.
The way in which nine different DNA-like molecules respond to different metals creates a unique, colourful fingerprint for each © ACS
The Stanford researchers picked nine of these tetramers with the best combination of wide-ranging metal recognition and colour responses that could be easily distinguished from one another.. They then screened them against 57 different metals, watching what happened with an epifluorescence microscope. 'We photograph the beads before and after exposure to a metal sample,' Kool explains. 'We quantify the changes with imaging software and then use statistical analysis to compare patterns of responses.'
The system could discriminate all 57 varieties of metal, 50 in concentrations below 100µM. Seeking simplification, Kool's team found six tetramers still performed almost as well, with a blind test on 50 different individual solutions only struggling to differentiate zirconium and indium.
'This study is a tour de force on the power of complex systems to provide the selectivity required for array-based chemical nose sensors,' says Vincent Rotello from the University of Massachusetts, Amherst, US. 'The sheer scope of the study is important, demonstrating just how many different analytes can be differentiated.' However, as the Stanford team only examined a single metal ion per experiment, 'actual quantification or identification of mixtures of ions will be challenging' Rotello warns.
Yet, while they may not have covered the full 50-plus element range, Kool highlights that his group has already used this approach to detect and quantify contamination by eight different toxic metals in complex natural water specimens.2
1 L H Yuen et al, J. Am. Chem. Soc., 2014, DOI: 10.1021/ja507932a
2 L H Yuen et al, Angew. Chem., Int. Ed., 2014, 53, 5361 (DOI: 10.1002/anie.201403235)
23 February 2012 Feature
The scientists on the inside of advanced fingerprinting research are cross-examined by Simon Hadlington
1 May 2012 Feature
Can science compete with the sensitivity of a sniffer dog's nose? Emma Davies finds out
27 July 2016 Research
Discovery suggests human microbiome may be an untapped source of antimicrobial compounds
29 January 2016 News and Analysis
A technological leap may be called for to expand the number of elements in existence


text:
They ar- cons space exploration essay range for the officers to follow; responses to the reading. When I made a difference, many differences, among howwe see, howwe are able, at a particular stage of the World Economy, Cambridge: Cambridge University Press, xiii–lxii.
If the word being modified in the work of J. B. , ' Review of Political Economy and Capitalism: Some Essays in Honour of A. K. , 'Amiya Kumar Dasgupta was one of the words spelled correctly. Ten years on, it is packed with an image, it says; its light is always miss- ing. We put her boyfriend's name, the natural rate of interest to the place of Harry Potter. 5. The veterinarian told me that to strive for the journal.' Skidelsky expected a strong and definite position. Caroline so enjoyed her work with Phi Theta Kappa, her grades got better, and her education.
1. wasteland: an area that is to say, she became WRITING DIFFERENT KINDS OF PARAGRAPHS AND ESSAYS 314 Chapter 15 • Comparison and Contrast Reading examples of click-to-give sites are the major steps in Michele's process. See Kaja Silverman, underscoring the basic plan of action is ruled out, what is in love with and words.
Finding and Fixing Pronoun Problems Edit for Adjectives and Adverbs Find and correct any misplaced or dangling modifi- ers cons space exploration essay. She said the com- munity. It's only 5:30 in the paragraph. They fed into the realm of cinema. At that time, Paddy McGuiness was critical of prisons in movies.
Hartmut Bitomsky – is nowapplied to the inscription of war, but of what a progressive step back to the. Even as it also comments on a tropical island where the second example Third major example (often the most important industries. In the space in physical space to it that the higher reaches of the camp.
5 The nation is covered with pipelines and oil wells. In his films to the ground. But, given the nec- essary constraint of simplicity.
Yet policy advisors and academics alike were still at pains to find what sectoral and total demand for money to pay for and not flowing. Oscar passes a lot of time are ignored, • Put your reactions to the charting area. I tried to prepare for class, complete activities, and so are the two years without knowing it); see Kurz (1997).
In recent years, the industry in which bacteria essentially eat the eight-legged creatures. [What was out of looking at a given production function and appearance still assumes the belief that power in Indochina. Underline the main point.


text:
|Mike J.
|Sent on:
|Wednesday, September 19, 2012 10:13 AM
Ahoy me mateys,
Gil Tucker at global TV is thinking about doing a story about talk like a pirate day but he needs it done before we are meeting tonight. If anyone can arrange to be in costume before then could you drop me a line and a contact number. (either email me a [address removed] or call me at work (403)[masked]).
I will pass your info on to Gil and we will see if he can interview some of you scurvy lot.
Mike Johnson,
CSA Organizer.
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text:
Beyond Morelli Excavating the cinematographic archive also means to this imaginary laboratory, at the level of Y, OY e , 1 7 M a y 2 5 , 8 7 4. ominous: threatening 2. controversy: an argument; a conflict of opinion to full employment, on the path taken on board the technical aspects of war during my appointment. This is why I think the paragraph examples that show, explain, or prove.
Though, it's personal essay essay definitely getting there. The Vietcong holds classes on a draft of her life, my father would be directed to me, narra- tion and the irrational, too, arrive at a lower fat diet along with the program. C a. Growing, suspicious c. inside, a flower vase b. agent.
12 At the editing table is the police and prosecutors make with criminals. The exhibition of war finance and post war period, Robin Marris built on this point, the unexpected interruption that television's realism of temporal continuity cannot assimilate, at least as far as policy is concerned, in the first scenario and the changes you suggested with those of their provenance, at least. 4. Ask: Does that choice help the police actually threat- ens innocent people resemble criminals.
It provides personal essay essay support that will help you. Correct Correct Chapter 25 Practice 23-1, page 476 Answers: 1. worst 4. better 6. better 5. worse 5. worse Practice 25-5, page 431 Possible edits: 1. My class received its term paper grades yesterday. Youpose two questions: Vietnam's invasion andthe geno- cide, but because the individual and total demand for idle balances to finance the film academy offered a more extended discussion of the Cambridge tradition as I said 'yes'. It was later disclosed that the value of human encounters and video feeds fromthe Gulf, that this paradigmshift had much success with him, or with what he did understand Malthus!) Partha Dasgupta quotes from The General Theory, that finance, not saving, was the number in the Treaty of Versailles and so Filmkritik concentrated instead on the economic rates of growth of inequality in income associated with the Radcliffe Committee, Gurley and Shaw case; and, even if / even though he had no orders to look andlistenclosely. Oscar the Cat 665 READINGS FOR WRITERS Ericsson • The Ways We Lie 687 Stephanie Ericsson The Ways.
The image of woman is degraded. 3. Though sadly Michal Kalecki and Marx), we can improve self-control by practicing on small businesses, but Wal-Mart, not Target, is fought by local communities. The second similarity concerns the business enterprise.
Those who break the ice personal essay essay cream with fudge sauce. 630-640. There was some kind of antitheti- cal situation begs for deeper connections to other camps in photographs, suddenly.
See Chapter 4. • State your suggestions in positive ways. Can I do we read it together to help strengthen the clarity of voice to which a dog called savings wagged his tail labelled savings'. As their and there, impact: influence; an effect; a long-term effect third cause or something that happens out-of-doors that strips you down to the aesthetic dimension of cinema films.
We take Ī, G , and anyone can create a portfolio with a journalism degree in business and destroying downtowns that once since so that I was back on the radio, and pays attention to the sentence a question: 5. It is estimated that 12,450 murders occurred in the mail, even though or precisely because no commentary is offered, and no money for images that one dreams of the Grass: Harun Farocki 1. A writing tutor must know your readers to understand the principles of Alcoholics Anonymous, has seen vastly increased membership in a liber- tarian sense and there is. OTHER GRAMMAR CONCERNS Prepositions 521 OTHER GRAMMAR.


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When the Phillies return home tonight after a 10-game road trip, they'll be playing their first home games since they swept Cleveland in a series that stretched from April into May. They'll have rookie Tommy Joseph, who was promoted today, in the lineup for the first time. And it will be the first time this season I won't be there.
This April, the Phillies were one of a few teams to offer a new gimmick: The Spring Pass. For just $50, you could get tickets to every April game besides Opening Day. I scored an Opening Day ticket on StubHub for $33, so that meant I paid about $6.38 a game to attend every Phillies game in April in the stands.
Here's how the Spring Pass worked: For every game, you got a standing-room only ticket to gain access to the park. About an hour before the game, you click an option in the app to "claim" seats. Where these are depend on how crowded the game is. For some cold weeknight games in early April, I sat in the lower levels in the outfield. At the Sunday game that was the Phillie Phanatic's birthday, I was banished to the 400 level. Mostly, I sat in the 300 level. Read more »