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BBQ ribs, pulled meats, burgers, sandwiches and Friday fish dinners make this a popular family friendly restaurant.
Historic Montgomery County Courthouse
This historic courthouse was built circa 1858.
Illinois Vietnam Veterans Memorial
This powerful granite monument and its eternal flame pay tribute to the 2,981 Illinois residents who served in the Vietnam War and died or are still missing. Located at Oak Ridge Cemetery.
This historic home is furnished with memorabilia from the early days of Montgomery County, and is open by appointment.
Trails for bird watchers and nature lovers; 200 acres.
Hard-to-find old records, tapes, CDs, furniture and lots of miscellaneous.
Choose and cut your own scotch pine, white pine, Austrian pine and other nursery trees. Shrubs, flowering trees and evergreens are also sold.
Serving contemporary, American cuisine.
China, silver, candles and miscellaneous gifts.
Luggage and accessories.
Traditional pub food, specializing in Horseshoe Sandwiches and Irish fare
Brand New Hotel offers a variety of rooms from standard double queen rooms to king and whirlpool tub suites. Relax in the hotel indoor heated pool and whirlpool or host your next meeting in our executive conference room.
Quality Inn & Suites - Litchfield
The Quality Inn & Suites is conveniently located on historic Route 66, only 45 miles from Springfield and several historic points of interest, including the Abraham Lincoln Presidential Library and Museum.
Baymont Inn and Suites - Springfield
Double, king, business king, leisure suite, whirlpool suite and executive board room which accommodates up to 25.
Authentic, Mexican cuisine.
Quality and authentic Italian flavor in homemade pizzas,calzones, pastas, salads and sandwiches.
Welcome travelers, wayfarers and refined persons. Celebrating 100 years, this historic inn is on the Courthouse Square in Hillsboro. Restaurant and bar at the facility.
Raymond Shoal Creek Golf Course
This nine-hole course is par 36.
Interior corridors, 3 floors, elevators, 104 rooms, guest laundry, truck parking, data ports in all rooms, free local calls, coffee 6am-10am, handicapped rooms, AARP discounts for members, children 17 and under stay free in parents room.
Covering 1200 acres, has picnic areas, marina and public boat slips.
Conveniently located just off I-55 at exit 100B, this beautiful hotel offers guest-friendly hospitality along with free breakfast, cable/HBO, local calls, newspaper, and parking. Ammenities include indoor pool, fitness center.
Featuring a wide variety of Asian specialties including sushi, teriyaki, Thai dishes and a full bar with speedy service and a pleasant dining area
Since 1991, stylish and price-savvy women like you have been raiding My Sister's Closet for your favorite labels. Louis Vuitton, St. John, Prada, Pucci, Chanel, Armani, Gucci, Hermes, Juicy, Vivienne Tam, True Religion, Antik Denim, Milly and Nanette Lepore,
Nick & Nino"s Penthouse Steakhouse
Perched 30 stories above historic downtown Springfield, Nick & Nino's provides a relaxed, inimate atmosphere in which to enjoy the finest food in Springfield. Located in the Hilton Springfield.
Northfield Inn Suites & Conference Center
Newer 107-room facility with adjoining conference facilities and own banquet kitchen & separate restaurant/bar. King/Queen jacuzzi rooms & suites, indoor pool, fitness center & continental breakfast. Also offer free HBO and USA today.
Holiday Inn Express - Litchfield
Conveniently located between Springfield, IL and St. Louis, MO. Near the historic Old Route 66. Litchfield Lake Lou Yeager boasts a beautiful lake, nature trails and picnic areas, 3 mi from the hotel. There are a variety of restaurants nearby.
Magic Kitchen presents fantastic Thai food. We are currently operating in two great locations throughout Springfield, one conveniently located downtown at 115 North Lewis Street and the other near the Fish Man Pet Center at 4112 North Peoria Road. We guarantee that you will leave with excitement and a full stomach.
Magic Kitchen presents fantastic Thai food. We are currently operating in two great locations throughout Springfield, one conveniently located downtown at 115 North Lewis Street and the other near the Fish Man Pet Center at 4112 North Peoria Road. We guarantee that you will leave with excitement and a full stomach.
Conveniently located off Interstate 55 at - less than 30 minutes south of Springfield attractions. Enjoy close proximity to shops and dining in downtown Litchfield, historic Route 66 attractions and outdoor recreation on Lake Lou Yaeger. Facility has 61 rooms with 2 suites. Free Wi-Fi and kids 17 and under stay free.
Two buildings with drive-up rooms on two floors.
Camp Butler is one of the 120 national cemeteries that served as a Civil War training camp, as well as a POW camp for Confederate soldiers. Call the cemetery office to schedule a tour.
Hamilton's Catering Service Inc.
Offering a variety of menus and services, and specializing in buffet style meals, but can also offer elegant plated meals. Since 1913.
Queen-size beds, HBO, ESPN, cable TV, free continental breakfast, guest laundry, children stay free with parents. Pets allowed with permission.
General antique shop.
Lincoln Souvenir and Gift Shop
Springfield and Lincoln souvenirs, jewelry, lots of miscellaneous gifts. Open March-November.
Sports Bar and a great place catch all the action for your favorite sports teams.
24-room property - two-story interior corridor hotel. Located near Lake Hillsboro, Glenn Shoals Lake and Coffeen Lake.
Toys, jewelry and lots of miscellaneous.
Women's clothing, candles, jewelry and gifts.
Mario's Italian Restaurant & Pizzeria
Casual family-style pizza parlor featuring a pizza lunch buffet, pastas, salads, sandwiches and other specialities.
A relaxed dining experience in an unique nostalgic setting. A Springfield tradition since 1988. Live entertainment Fri and Sat night.
Housewares and a variety of items.
Newly rennovated hotel with meeting facilities for groups uo to 200 people, outdoor pool, studio suites, free parking and scenic setting.
Hillsboro Sherwood Forest Campground Montgomery County
Camping, Hiking, Fishing along with many special events. Call ahead for information.
This open air market specialized in items prior to 1980 including: antique, vintage, and refurbished items. Hundreds of vendors flood the streets of Downtown Litchfield every second Sunday of the month from April to October from 9 AM to 3 PM, beginning on the corner of Union Avenue (Route 16) and State Street. Food is available and live entertainment happens all day in Library Park.
Many sites and attractions in historic Springfield will be brimming with free live performances and fun activities that will transport you and your family back in time – to the Springfield Abraham Lincoln knew and loved. From storytelling and interaction with costumed interpreters to visits with Mr. Lincoln, himself, this is a trip to the past you won't want to miss! History Comes Alive will run through August 13th.
Cooper's Hawk Winery & Restaurant - Springfield
After four years of planning and construction, Cooper's Hawk Winery & Restaurant opened in 2005 in Orland Park, a south suburb of Chicago. The concept was unique. We would create a signature menu with a selection of dishes designed to pair perfectly with our own Cooper's Hawk wines, made in our own private winery. With sophisticated food and exceptional wines, our concept was born. The Cooper's Hawk concept includes four distinct components: an upscale casual dining restaurant, full-service bar, private barrel-aging room, and Napa-style tasting room and retail gift store…all under one roof. Each Cooper's Hawk location is distinctive in layout and décor, but carries the same warmth and elegance that guests have come to expect.
This event will take you back in time with a historic re-enactment of military life from 1750-1820. Military and camping demonstrations will be provided daily. Both campers and public are welcome.
Route 66 Half Marathon, 5K, Mile Dash
Run down Historic Route 66 in this race down memory lane. On this flat course, you will have the opportunity to set a personal best. Plus, its filled with some of the oldest landmarks on Historic Route 66.
Try your hand at the Litchfield TriathLou at Lake Lou Yaeger. Swimming in Lake Lou Yaeger is the best open water swim you will have in the Midwest. There really is no comparison to this large beach with pristine flat racing water. The bike course is flat and fast with some great curves to keep you entertained the whole ride. Both the 5k and 10k runs see very honest mile paces with plenty of flats, and a couple hills throughout. Sprint and Olympic distances are available.
This remote control jet rally gathers some of the best scale model jets in the country. Live stunts and performances will be available to the public.
Enjoy 10 days of thrills and excitement at this family-friendly fair featuring world renowned concert artists, food, carnival rides, livestock shows, agricultural exhibits, contests, educational displays, free entertainment, harness racing, auto racing and much more.
Along a peaceful country road, just minutes from Illinois' capital, you will find one of the most unconventional and unpretentious destinations around. This is home to Danenberger Family Vineyards, where our winery dogs will greet you and you will be treated less like a visitor and more like a friend. When you visit Danenberger Family Vineyards will enjoy memorable experiences that stimulate and engage the senses. Our passion for grapes, wine and food is a part of our lifestyle, and we love to share it.
CITY:New Berlin
Custom Cup is a roast-to-order coffee service in Springfield, IL. They specialize in small batch hand selected beans from around the world, roasted per customer order, and delivered to your door or shipped nationwide.
Chairs on Parade is an event where Carlinville businesses purchases a chair and decorates it to their liking. The event is all about bringing old fashioned fun, family and shopping together while supporting the local business community. The chairs will be on display outside of the businesses beginning on Saturday, August 6th. Part of the event includes the first ever Carlinville Restaurant Week from Monday, August 22nd through Sunday, August 28th, celebrating local food establishments and retail stores. The highlight of that week will occur on Friday, August 26th with a flotilla parade and afterwards an auction for chairs that are donated back to Shop Local First. The profit from the chairs will go to the local food bank. The night will also include Mid-West Largest Squirt Gun fight, and a street party with Captain Geech & The Shrimp Shack Shooters rock band.
Celtic Mist Pub is a home away from home for both locals and visitors alike. The atmosphere has a touch of nostalgia yet it is an alluring new rendezvous for today's modern crowd. The owners, John and Margaret O'Riordan pride themselves on welcoming everyone and providing an atmosphere where friends will gather to chat, laugh and enjoy the banter of the goings-on.
Our craftsmen consist of local Firefighters and Veterans who meticulously dismantle these barns, which have stood as a sign of progress and livelihood for families in rural America for over 100 years. Our Craftsmen then take special care to clean and prepare the wood while retaining the strength and beauty that gives it the unique appeal that cannot be imitated. These barns have stood as a symbol of the American Spirit for generations. Let them continue to stand, in your home, for generations to come.
Vixen Hill Winery is a family-owned, wine-making estate with the vision to create a special destination for hand-crafted wines and warm hospitality in Central Illinois. The winery produces well-balanced wines that pair well with everyday foods.
President Abraham Lincoln Springfield - a DoubleTree by Hilton Hotel
All of the beautiful guest rooms and suites at this hotel in Springfield, IL, feature modern amenities and thoughtful touches including a flat-screen TV, premium bedding, and complimentary WiFi access. Work out on the latest equipment in the 24-hour fitness center or swim in the indoor pool. Start your day with a hearty Midwestern breakfast buffet at Lindsay's Restaurant and visit The Globe Tavern to enjoy hand-crafted specialty drinks, premium draft beers and live sports on seven flat-screen TVs. Host a conference at this Springfield, IL hotel with a 24-hour business center and 13,000 sq. ft. of flexible meeting space, ideal for a variety of events for up to 600 guests.
Engrained Brewing Company is a locally owned micro-brewery and farm to table restaurant. Brewed onsite, our beer lineup is crafted to bring friends together with a broad range of styles. Our menu features fresh and affordable food from local family farms. Our brewmaster is always crafting fresh new brews to give you a unique experience with every visit. Gather at Engrained for a drink with friends, a family dinner, or special event at your own local brewpub!


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The authors have no conflict of interest.
DMP1 Depletion Decreases Bone Mineralization In Vivo: An FTIR Imaging Analysis†
Version of Record online: 22 AUG 2005
Copyright © 2005 ASBMR
Journal of Bone and Mineral Research
Volume 20, Issue 12, pages 2169–2177, December 2005
Additional Information
How to Cite
Ling, Y., Rios, H. F., Myers, E. R., Lu, Y., Feng, J. Q. and Boskey, A. L. (2005), DMP1 Depletion Decreases Bone Mineralization In Vivo: An FTIR Imaging Analysis. J Bone Miner Res, 20: 2169–2177. doi: 10.1359/JBMR.050815
- †
Publication History
- Issue online: 4 DEC 2009
- Version of Record online: 22 AUG 2005
- Manuscript Accepted: 15 AUG 2005
- Manuscript Revised: 28 JUL 2005
- Manuscript Received: 12 JAN 2005
- Abstract
- Article
- References
- Cited By
- dentin matrix protein-1;
- Fourier transform infrared imaging;
- mineralization;
- osteomalacia model;
- mouse;
- bone geometry
The role of DMP1 in mineralization was analyzed by comparing bone mineral and matrix properties in dmp1-null female mice to heterozygous and wildtype controls by FTIR imaging spectroscopy. The observed decreased mineral content in dmp1 null mice indicates a key role for dmp1 in bone mineralization. Indirect effects of DMP1 on other systems also determine the KO phenotype.
Introduction: Dentin matrix protein 1 (DMP1), an acidic phosphorylated extracellular matrix protein, is highly expressed in mineralized tissues. In vitro, DMP1 peptides can promote or inhibit mineralization depending on the extent of phosphorylation, the peptide size, and concentration. To clarify the biological function of DMP1 protein on in vivo mineralization, this study analyzed bone properties of dmp1 knockout (KO) mice compared with heterozygous (HET) and wildtype (WT) controls.
Materials and Methods: Tibias from dmp1 KO and age-, sex-, and background-matched HET and WT mice at 4 and 16 weeks (Ntotal = 60) were examined by Fourier transform infrared imaging (FTIRI), histology (n = 6 per genotype and age; N = 36), and geometry by μCT (n = 4 per genotype and age; N = 24). Serum ionic calcium and phosphate concentrations were also determined.
Results: The mineral-to-matrix ratios (spectroscopic parameter of relative mineral content) were significantly lower in dmp1 KO mice tibias compared with WT and HET at 4 and 16 weeks. The mineral crystallinity (crystal size/perfection) was significantly increased in dmp1 KO and HET mice relative to WT. Collagen cross-link ratios (a spectroscopic parameter related to the relative amounts of nonreducible/reducible collagen cross-links) in dmp1 KO were not significantly different from WT and HET. Based on μCT, cortical bone cross-sectional areas at 16 but not 4 weeks were significantly reduced in the KO compared with controls. Maximum, minimum, and polar cross-sectional moments of inertia were significantly lower in dmp1 KO than in HET at 16 weeks but not at 4 weeks. Histological analysis and μCT 3-D images suggested that dmp1 KO mice had osteomalacia. Dmp1 KO mice had significantly lower ionic calcium and phosphate concentrations relative to WT, whereas in the HET, values for phosphate were equivalent, and calcium values were decreased relative to WT values.
Conclusions: The findings of decreased mineral-to-matrix ratio and increased crystal size in bones of dmp1 KO mice suggest that DMP1 has multiple roles (both direct and indirect) in the regulation of postnatal mineralization. We suggest that direct effects on mineral formation, crystal growth, and indirect effects on regulation of Ca × P concentrations and matrix turnover all contribute to the dominant phenotype in the dmp1 KO mouse.
DENTIN MATRIX PROTEIN-1 (DMP1), initially named AG1,(1) an acidic noncollagenous phosphoprotein, is a member of the SIBLING (small integrin binding ligand N-linked glycoprotein) family,(2) which includes osteopontin, bone sialoprotein, DMP1, dentin sialophosphoprotein (DSPP), enamelin, and matrix extracellular phosphoglycoprotein (MEPE). These proteins share some common features such as their location on human chromosome 4q21, their multiple phosphorylation sites, their highly acidic nature, and the presence of an arginine-glycine-aspartate (RGD) cell attachment domain. Each of these proteins is thought to play an important role in tissue mineralization.(1,3,4)Dmp1 was first cloned from dentin(1) and later found in bone,(5–7) cartilage,(8) and cementum.(9) Thus, it was originally thought to be specific to mineralized tissue. Recently, however, DMP1 was detected in a number of nonmineralized tissues including liver, muscle, pancreas, kidney, brain,(5,10) and salivary glands,(11) as well as in human lung adenocarcinoma,(12) phosphaturic mesenchymal tumors,(13) and cancers of the breast, uterus, and colon.(14) DMP1 is a multifunctional protein that has been found to regulate cell attachment(15) and cell differentiation,(16,17) to activate matrix metalloproteinase-9,(18) and has been postulated to play a significant role in biomineralization.(19)
In vitro, DMP1 acts as a hydroxyapatite (HA) crystal nucleator with very high calcium ion binding capability(20) and binds specifically to the N-telopeptide region of type I collagen in the gap region.(21) In addition, studies of fetal rat calvarial cell cultures showed that the expression of DMP1 is associated closely with "bone nodule" formation and mineralization.(8) These studies suggest that DMP1 is a promoter of mineralization. However, in a gelatin-gel diffusion system,(22) different forms of DMP1 act as nucleators or inhibitors. In the gelatin-gel system, the nonphosphorylated rDMP1 and the highly phosphorylated C-terminal 57K peptide of DMP1 act as apatite nucleators, the phosphorylated full-length bovine DMP1 expressed in marrow stromal cells is a mineralization inhibitor, and the rDMP1 phosphorylated in vitro had no effect on HA formation and growth.(23) Thus, further study is needed to understand the in situ effects of DMP1.
In vivo studies showed that embryo and newborn dmp1-null mice could not be distinguished visibly from wildtype littermates by whole skeleton radiography and Alizarin red/Alcian blue staining; femora of dmp1-null mice embryos did not show obvious abnormalities by H&E staining, although the dmp1-null mice had slightly expanded hypertrophic zones and modestly increased bone diameter compared with wildtypes.(24) Ye et al.(19) recently reported that dmp1 null mice postnatally displayed a profound tooth phenotype characterized by increased width of predentin with a reduced dentin wall thickness and dentin hypomineralization. In addition, vertebrae and long bones in postnatal dmp1-null mice were shorter and wider with delayed and malformed secondary ossification centers and an irregular and highly expanded growth plate, creating a phenotype resembling dwarfism with chondrodysplasia,(25) suggesting that DMP1 is critical for the postnatal development of mineralized tissues.
The purpose of this study was to clarify the role of DMP1 in bone mineralization in vivo. We used Fourier-transform infrared imaging (FTIRI) and μCT to characterize changes in both the mineral and matrix of the bones of dmp1 knockouts (KOs) and age-, sex-, and background-matched wildtypes (WTs) and heterozygotes (HETs). FTIRI, in which an array detector is coupled to an infrared microscope allowing acquisition of multiple spectra simultaneously from selected areas of bone sections, provides unique spatially resolved information on the amount of mineral present, mineral crystallinity, and collagen properties. High-resolution μCT was used to provide 3-D information on bone geometry.
Experimental animals
Dmp1-null mice targeted to replace the dmp1 exon 6 with a lacZ knock-in gene and a neo-cassette in embryonic stem cells were developed at University of Missouri-Kansas City as described elsewhere.(24) The heterozygous dmp1-null mice, verified by Southern blot analysis, were interbred to generate homozygotes in the C57BL/6 background. The Institutional Animal Care and Use Committees of University of Missouri-Kansas City and the National Institute of Environmental Health Sciences, NIH, approved all animal experimental protocols.
Ten tibias per genotype per age were obtained from WT, HET, and KO female mice at 4 and 16 weeks (Ntotal = 60). Four tibias per group were cleaned of soft tissue, stored in 90% ethanol, and scanned by μCT as detailed below. The proximal ends of the other six tibias were cut off and embedded in Spur's medium (Electron Microscopy Science, Hatfield, PA, USA). Longitudinal nondecalcified sections of the proximal ends of the tibia (four sections per bone) were cut by a HM360 microtome (Microm, Walldorf, Germany) at 2 μm thickness and mounted on barium fluoride (BaF2) infrared windows (Spectral Systems, Hopewell Junction, NY, USA) for FTIRI analyses. Another two sections per bone were cut at 4 μm thickness and stained by the von Kossa method and counterstained with neutral red(26) and Goldner's trichrome.(27)
Tibial geometry was determined from μCT (Enhanced Vision Systems Model MS-8 In Vitro μCT scanner; GE Healthcare, London, Ontario, Canada). Tibias, cleaned of soft tissue, were scanned in saline (0.9% sodium chloride; Baxter Healthcare, Deerfield, IL, USA). 2-D projections of four tibias per scan were collected by Evolver software (GE Healthcare); isotropic voxel size was 12 × 12 × 12 μm. The hardware of the Enhanced Visions System μCT scanner is engineered to minimize beam hardening associated with polychromatic X-ray to ensure uniformity of the beam at the detector array. Filtering of the low-energy portion of the X-ray spectrum with aluminum, acrylic, and a saline bath results in a flat field where the attenuation within a uniform material is independent of spatial position. To minimize noise, a large number of views, increased frame averaging, and increased shutter exposure time were used, resulting in 4-h scans. Reconstruction of projections into CT volume data were accomplished by Beam software (GE Healthcare) with a modified Parker algorithm.(28,29) 2-D images, 3-D volume generation, and threshold analysis were calculated with Microview (GE Healthcare). Reconstructed grayscale volumes for each cortical subvolume at the middle of the tibias were extracted and segmented with the individual threshold defined by the auto-threshold function of Microview, namely generating the attenuation histogram of the volume of interest and fitting the data with the Otsu method.(30) We used the auto-threshold method because it segregates mineralized tissue based on the distribution for a given specimen of background and mineralized voxels and helps to reduce experimental error associated with temporal effects. A MATLAB (version 6.5; Mathworks, Natick, MA, USA) program based on an integrative method(31) was used to calculate the cross-sectional area, maximum cross-sectional moment of inertia (Imax), minimum cross-sectional moment of inertia (Imin), and polar cross-sectional moment of inertia (J). Moment of inertia is a geometric property of a beam that measures the distribution of mineral about a given axis, representing the ability to resist bending or torsion.
Tibia sections of 4- and 16-week-old mice were examined by FTIRI to acquire spectral images using either the BioRad FTS-6000 Stingray system (BioRad, Cambridge, MA, USA) or the Perkin Elmer Spotlight Imaging system (Perkin Elmer Instruments, Shelton, CT, USA). The spectral resolution was 16 cm−1, and areas 400 × 400 μm2 were examined on both instruments. The spatial resolution was ∼7 μm. Spectra were transferred to yield images corresponding to infrared band areas, peak height ratios, and integrated area ratios by a combination of instrument software and ISYS Chemical Imaging Software (v 2.1; Spectral Dimensions, Olney, MD, USA).(32,33) Background spectra were collected under identical conditions from the same BaF2 windows. IR data of three areas per anatomical bone site at primary spongiosa, secondary spongiosa, and cortex were collected (Fig. 1). After acquisition, spectra were truncated to allow analysis of the spectral data of interest and zero-corrected for the baseline, and the spectral contribution of Spur embedding media was subtracted using ISYS software.
Three spectroscopic parameters were calculated: mineral-to-matrix ratio, crystallinity, and collagen cross-link ratio (XLR). The mineral-to-matrix ratio (v1, v3 PO4 band [900–1200 cm−1]/amide I band [1590–1720 cm−1] integrated areas ratio) is a measure that corresponds to ash weight measurements.(34) Mineral crystallinity is a parameter that corresponds to the crystallite size and perfection as determined by X-ray diffraction, and was calculated from the intensity ratios of subbands at 1030 (stoichiometric apatite) and 1020 cm−1 (nonstoichiometric apatite).(33) XLR is a parameter reflecting the relative ratio of nonreducible and reducible collagen cross-links, expressed as the absorbance ratio at two specific wavenumbers (1660 and 1690 cm−1).(35) Details for the spectral processing methods and reproducibility of measurements are published elsewhere.(31,32,34–36) In the spectral images, pixels devoid of bone (no mineral and/or matrix spectral signature) were set equal to zero and masked to be excluded from calculations. The spectroscopic results were expressed as histograms describing the pixel distribution of the three parameters above, mean values, and SDs of the pixel distribution, and corresponding color-coded images were generated at the same time by ISYS. Means and SDs were averaged for multiple sites in each animal and among six different animals for each age and genotype using Microsoft EXCEL.
Serum obtained by the cardiac puncture from WT, HET, and KO animals was analyzed for calcium and phosphate content by ion chromatography (DX-120 ion chromatograph; Dionex, Sunnyvale, CA, USA) as described elsewhere.(37)
Statistical analysis
Departures from normal distribution were assessed graphically and by the Kolmogorov-Smironov test. None of the outcome variables departed from normality. Data were analyzed by two-factor ANOVA with interaction. The first factor was genotype (dmp1 WT, HET, and KO) and the second factor was age (4 and 16 weeks). The ANOVA type I error rate was set at 0.05. If the interaction term between genotype and age was significant, the conclusion was drawn that the effect of genotype was dependent on age. This gave justification for examining effects of genotype separately for each age.(38) Posthoc comparisons were made using Tukey pairwise mean comparison tests. The Tukey experiment-wise error rate was set at 0.05. If the ANOVA interaction term was not significant, the main effects were examined. If the main effect of genotype was significant, contrasts among levels of genotype were tested. The contrast error rate was set at 0.05. Analyses were conducted using SYSTAT @ 9 Statistics I (SPSS, Chicago, IL, USA).
Dmp1 KO mice showed evidence of osteomalacia
In histological analysis, morphologic changes characteristic of osteomalacia were observed in the tibia sections of dmp1 KO mice: widespread excessive osteoid and increased width of osteoid seams at the surfaces of both cancellous and cortical bone, structure deformities, smaller and disorganized trabeculae, irregular and expanded growth plate, enlarged metaphysis, smaller secondary ossification center, and considerable cortical porosity (Fig. 1).
Geometric parameters were decreased in diaphysis of dmp1 KO mice tibias
Proximal tibias of dmp1 KO mice were shorter and wider than those of dmp1 WT and HET by μCT, with enlarged flaring metaphysis, expanded growth plates, smaller secondary ossification center, fewer trabeculae, thinner diaphyseal cortical walls, and larger marrow space (Fig. 2).
Cross-sectional areas of the tibial midshaft were significantly smaller in dmp1 KO mice than in both dmp1 WT and HET mice at 16 weeks of age (Table 1). The cross-sectional moments of inertia including Imax, Imin, and J were significantly lower in dmp1 KO than in dmp1 HET at 16 weeks. No significant differences in these parameters were found comparing dmp1 WT and KO mice. In WT and HET mice at 16 weeks, cross-sectional areas and moments of inertia were larger than those of corresponding genotype mice at 4 weeks, indicating growth. In the dmp1 KO mice, only the cross-sectional area increased with age from 4 to 16 weeks; the moments of inertia did not get larger.
Mineral-to-matrix ratio decreased significantly in dmp1 KO mice
As shown in Fig. 3, at 4 and 16 weeks, mineral-to-matrix ratios of dmp1 KO mice in the cortex, primary spongiosa, and secondary spongiosa were significantly lower than those of WT and HET mice. Interestingly, the mineral-to-matrix ratio of 16-week dmp1 KO mice increased significantly from 4-week values, whereas WT and HET mice did not show significant increases in this parameter in primary and secondary spongiosa. These findings are shown by representative color-coded images of mineral-to-matrix ratio at cortex and secondary spongiosa of all genotypes at both ages (Figs. 4A and 4B).
Crystallinity increased in dmp1 KO mice
Crystallinity, a measure of crystal maturity, a parameter that increases with bone age in normal animals, was significantly higher in the dmp1 KO mice with respect to WT mice at 4 and 16 weeks at all bone sites (Fig. 3B). However, there was not a significant age-dependent effect. Interestingly, this parameter was greater in the HET than in the KO mice at 4 and 16 weeks for cortical bone and was significantly greater than WT mice but not significantly different from KO mice for HET primary spongiosa and secondary spongiosa. Representative FTIRI images of 1030/1020 ratio at cortex and secondary spongiosa of all genotype mice at both ages show the intensity and spatial distribution of crystallinity in the same color scale (Figs. 4C and 4D).
Collagen cross-link ratio did not change in dmp1 KO mice
The collagen cross-link parameter, XLR, calculated from the intensity ratio 1660/1690 wavenumbers, were not significantly different in the dmp1 KO mice bones compared with values in similar bone sites of WT and HET mice at both ages (Fig. 3C).
Serum chemistry
Serum calcium and phosphate concentrations in mice of intermediate age (12 weeks) are summarized in Table 2. The KO mice had significantly lower ionic calcium and phosphate concentrations relative to WT mice, whereas in the HET mice, values for phosphate were equivalent and calcium values were decreased relative to WT values.
Bone is a unique mineralized tissue composed of several cell types and a structured organic matrix impregnated with oriented hydroxyapatite crystals. Bone cells synthesize and secrete the bone matrix, consisting of type I collagen and noncollagenous proteins.(4) Although the detailed mineralization mechanism remains obscure, phosphorylated noncollagenous proteins including the SIBLING proteins have been shown both to nucleate formation of apatite crystals within collagen gap zones and to block the formation and growth of these crystals.(3,4,21,39–41) DMP1, a member of the SIBLING family, has very high calcium ion and apatite binding capabilities,(20) is secreted into the extracellular matrix during endochondral bone formation,(42) and may have a role in initial mineralization.
This study has shown that deletion of the dmp1 gene in mice results in bone changes reminiscent of those noted in osteomalacia,(43,44) decreased bone mineralization, and increased mineral crystallinity. Dmp1-null mice bones are less mineralized than their age-, background-, and sex-matched WT and HET counterparts, as reflected by a consistently decreased mineral-to-matrix ratio by FTIRI. Furthermore, the mineral crystallinity, as estimated from the 1030/1020 wavenumbers intensity ratio, was increased in the dmp1-KO mice bone relative to the corresponding WT mice. In addition, 16-week dmp1-KO mice had smaller cross-sectional area than dmp1 WT and HET mice and had lower cross-sectional moments of inertia compared with the HET but not with the WT mice.
The dmp1-KO mice had flared metaphyses, thinner cortices, and reduced diaphyseal moments of inertia that did not increase with age. This, as well as the decreased mineral content, would make the bones weaker. Whereas the widening of the metaphysic might be a response to the decreased mineralization, the failure of the bones to grow most likely is directly caused by the mineralization defect, although it might relate to the role of dmp1 in regulating bone cell differentiation.(45)
These findings support a role for DMP1 or DMP1 peptides in the mineralization process. Qin et al.(46,47) isolated two fragments of DMP1 protein from bone, namely the N-terminal 37-kDa fragment and C-terminal 57-kDa fragment, and indicated that DMP1 is proteolytically cleaved at four X-Asp bonds. Isolation of the complete autochthonous form of DMP1 protein has not been successful. The carboxyl-terminal 57-kDa fragment of DMP1 and the unphosphorylated recombinant protein acted as nucleators of hydroxyapatite.(20,21,23) In contrast, a phosphorylated full-length bovine DMP1 expressed in marrow stromal cells, and presumed to represent the actual intact DMP1 made in bone cells, was a mineralization inhibitor.(23) We thus speculate that, as the complete phosphorylated DMP1 is secreted into extracellular matrix, some proteinases cleave it into fragment(s) that initiate mineralization. Analogously, DSPP, another SIBLING member, considered to be an inactive precursor protein, is cleaved at X-Asp bonds to release a functional C-terminal fragment, dentin phosphoprotein (DPP), which is an extremely effective nucleator of hydroxyapatite.(3,39,46,48) It should be noted that the enzymes responsible for DMP1 proteolysis have not been conclusively identified, although BMP-1/Tolloid-like proteinases(49) and phosphate regulating gene with homologies to endopeptidases on the X chromosome (PHEX) enzyme(46) have been postulated to be involved. Steiglitz et al.(49) have successfully used BMP-1/Tolloid-like proteinases to cleave full-length DMP1-generating fragments similar in size to those previously isolated from bone and showed that fibroblasts from mouse embryos lacking this proteinase have deficient DMP1 processing.
In addition to its postulated function as a hydroxyapatite nucleator, DMP1 also is involved in calcium and phosphate metabolism through the kidney.(10) A kidney effect is suggested by the significantly reduced serum concentration of calcium and phosphate in the dmp1-KO as contrasted with the WT and HET mice. The kidney effect would have an indirect impact on mineralization but could account for much of the observed phenotype because lower Ca × P products, as seen in rickets and osteomalacia, would decrease the formation of new mineral and would tend to increase crystal size, because it is energetically easier for whatever crystals are present to grow in the presence of low Ca × P products than for new crystals to form.(50)
Similarly, because DMP1 can bind pro-MMP-9 to activate MMP-9,(18) an additional indirect effect that could account for the hypomineralization phenotype would be the need for DMP1 activation of MMP-9 for matrix modification before calcification. There are other activators of MMP-9, so this could be a minor effect. However, the MMP-9 KO also has impaired mineralization associated with blocked vascular invasion,(51) although frank osteomalacia has not been reported in MMP-9 KO mice, and there are other DMP1-independent pathways that can be used for MMP-9 activation.(18)
The increased crystallinity noted in the dmp1-KO mice in part can help distinguish between these mechanisms. The hydroxyapatite crystals formed in the presence of 57-kDa C-terminal DMP1 fragments were smaller than those formed in its absence,(23) indicating that DMP1 can regulate crystal growth. Thus, it is possible that the crystals are larger in the dmp1-KO mice because a regulator of growth is missing or because nucleation is impaired and thus fewer nuclei are available. Because there is a tendency for existing crystals to grow rather than for new crystals to form, especially in an environment in which Ca × P product is reduced, this could account for the increased crystallinity in the dmp1-KO mice. Crystals in animals with decreased or modified proteoglycans tend to be larger,(52,53) suggesting that the failure to activate MMP-9 probably is not a key factor in the mineralization defect in these animals, although it may account for the unusual shape of the growth plate, because vascular invasion would be impaired if MMP-9 were not activated. The lack of detectable difference in matrix age, reflected by XLR, implies that the matrix may not be persisting for a longer time, although the scatter in the data indicates that the matrix distribution in the WT mice tends to be much more heterogeneous than in the KO mice, implying that the KO matrix might be processed to different extents.
It is important to note that values for crystallinity in dmp1 HET mice were not intermediate between values for KO and WT mice, but were greater than KO values. This implies that this is a dominant trait and stresses the importance of dmp1 expression for normal development. Serum Ca and P levels in HET mice were intermediate between WT and KO mice; thus, the alterations in crystal size are probably not directly caused by a physicochemical effect, but are more likely dependent on direct actions of DMP1.
In conclusion, the findings of decreased mineral content and increased crystal size in dmp1-KO mice show depletion of DMP1 in vivo decreases bone mineralization and reveal DMP1 has multiple roles (both direct and indirect) in the regulation of postnatal mineralization. We suggest that direct effects on mineral formation and mineral crystal growth and indirect effects on regulation of Ca × P concentrations and matrix turnover all contribute to the dominant phenotype in the dmp1-KO mouse.
The authors thank Christopher Fritton for assistance with the μCT and MATLAB program and Dr Stephen B Doty and Orla O'shea for assistance with the histology. This work was supported by National Institutes of Health Grants DE-04141 (ALB), AR0476121, and AR051587 (JQF). This study was conducted in a facility constructed with support from Research Facilities Improvement Program Grant C06-RR12538 from the National Center for Research Resources. The imaging data was obtained in the HSS Musculoskeletal Core Center (AR0476121). HFR is a recipient of a T32 fellowship (DE07294).
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- 162001 Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1. Proc Natl Acad Sci USA 98: 4516–4521., , , , ,
- 172004 Dentin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFP-transgene. Bone 35: 74–82., , , , , , , ,
- 182004 Three small integrin binding ligand N-linked glycoproteins (SIBLINGs) bind and activate specific matrix metalloproteinases. FASEB J 18: 734–736., , ,
- 192004 Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J Biol Chem 279: 19141–19148., , , , , , , ,
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- 331999 Infrared spectroscopy, microscopy, and microscopic imaging of mineralizing tissues. Spectra-structure correlations from human iliac crest biopsies. J Biomed Opt 4: 14–21., ,
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- 371999 Renal sulfate secretion is carbonic anhydrase dependent in a marine teleost, Pleuronectes americanus. Am J Physiol Renal Physiol 276: F288–F294., , ,
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- 411995 Osteopontin and related phosphorylated sialoproteins: Effects on mineralization. Ann NY Acad Sci 760: 249–256.
- 422001 Dentin matrix protein 1 is predominantly expressed in chicken and rat osteocytes but not in osteoblasts. J Bone Miner Res 16: 2017–2026., , , , , ,
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- 452004 Transcriptional regulation of dentin matrix protein 1 by JunB and p300 during osteoblast differentiation. J Biol Chem 279: 44294–44302., , , , , , ,
- 462003 Evidence for the proteolytic processing of dentin matrix protein 1. Identification and characterization of processed fragments and cleavage sites. J Biol Chem 278: 34700–34708., , , , , ,
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- 521992 Treatment of proteoglycan aggregates with physeal enzymes reduces their ability to inhibit hydroxyapatite proliferation in a gelatin gel. J Orthop Res 10: 313–319., , ,
- 531991 Hydroxyapatite formation in the presence of proteoglycans of reduced sulfate content: Studies in the brachymorphic mouse. Calcif Tissue Int 49: 389–393., , ,


text:
I just had the instrument cluster replaced and now am told the heater core needs replacing. Could this possibly make the new instrument cluster malfunction?
heater core replacement on 1991 Mercedes-Benz 500SL
Get Your Car Fixed at a RepairPal Certified Shop.
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Not unless it is spraying the cluster or its electrical connections with coolant. Please keep in ming that the vapors from antifreeze are toxic and you should not breath them- driving a car with a bad core could expose you to health risks! Greg


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Our Prime Directive . . .Serving hamburgers prepared with pride and care using Naturally Raised Black Angus Beef. This is beef raised with no hormones or antibiotics and primarily grass fed on family-owned sustainable ranches. Throughout our menu we use the highest quality ingredients, in their purest form, often organic and from local sources.
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Kennedy Plaza For Lunch, Dinner At Promenade Apartments / Guest Tip War Today
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text: Hi, Do we have any examples, tutorials of using VBO? Mike, I have seen some articles on your blog. I would be interested to test VBO using pyOpenGL. I have already done some tests using display list (~1 million polygons of fluid simulation) and I would like to compare it using VBO. Prashant Connect with friends all over the world. Get Yahoo! India Messenger at http://in.messenger.yahoo.com/?wm=n/


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Police: Man broke $2,400 signMarch 02,2013BENNINGTON A Farley Drive man was charged with a felony Monday after police said they believed he broke a wooden sign on Elm Street on Feb. 1 that cost nearly $2,400 to replace.
Matthew Bortell, 27, of Bennington, pleaded innocent in Bennington criminal court to a felony count of unlawful mischief resulting in more than $1,000 in damage.
In an affidavit, Officer Thalia Hudson, of the Bennington Police Department, said she was dispatched about 2:45 a.m. Feb. 1 to Elm Street. At the scene, Hudson spoke to a woman who said she had heard the sign for Edwards Investments being broken and saw two men leaving the area.
Hudson said Bortell was spotted on Main Street a short time later. While Bortell admitted to being on Elm Street earlier in the morning with another man, Hudson said he wouldnt give police a last name or address for the other man and denied breaking the sign.
Staff at Edwards Investment gave police an estimate of $2,390.46 to replace the sign.
Bortell has a previous conviction for unlawful mischief resulting in more than $1,000 damage from November 2009.MORE IN This Just InPARIS You cant put a guard in every church and patrol every beach. Full StoryA New Hampshire man has been arrested for possession of heroin following a traffic stop on I-91... Full StoryGROTON State police have arrested a Groton man for a drug-related death that occurred at his... Full Story
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Report: 7-11 dumps Citgo (Update: Dropped before)
posted at 10:34 pm on September 26, 2006 by Ian
WFLA of Orlando, FL is reporting:
BHL has obtained a copy of a letter from Janey Carpenter, Manager of Consumer Affairs that states 7-11 Stores are ending their 20-year supply agreement with CITGO Petroleum Corp. According to Carpenter, the contract ends next week and 7-11 is now making the switch to its own branded gasoline. The company says the change has nothing to do with derogatory comments made last week by Venezuelan President Hugo Chavez at the United Nations. Nevertheless, 7/11 Stores will begin selling in October gas provided by US companies, such as Tower Energy Group in Torrance, CA., Sinclair Oil of Salt Lake City and Frontier Oil Corp of Houston. Carpenter says the signs will start to change, with CITGO signs coming down from the stores' gas canopy and off gasoline dispensers. More than 2,100 stores are affected. While most of the signs will be changed by end of '07, not all CITGO signs will be off gas canopies until '08.
Update: Susser Holdings dropped Citgo a few weeks ago:
Valero's gain is CITGO's loss. In awarding Valero a virtual supply exclusive on its 320-plus company-operated stores, Susser Holdings Corp., Corpus Christi, Texas, is radically downsizing its 18-year relationship with CITGO, a company that is undergoing a significant realignment of its own.
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Product Description
Adapted from an Elmore Leonard novel, the script is hardly subtle or original and it never makes the gaping chasm between the search for justice and the bloody reign of terror work in any dramatic terms. It's a rather amorphous morality tale without a solid grounding, but it's dutifully violent, charged with righteous anger, and makes good use of the stark landscape (it was shot in Spain, giving it even more of the flavor of a spaghetti Western). It's a bit embarrassing how Hollywood puts the spotlight on racism by casting a white actor to play the wronged minority, but Lancaster creates a wonderful character in Valdez. With gentle eyes and a tired smile behind a face covered in brown Hollywood makeup, he brings sure-footed authority and calm ruthlessness to a mission of justice by a man wronged one too many times. --Sean Axmaker


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In-depth Study – Coming Fall 2016…
St. Augustine Parish offers several opportunities each year to study God's Word and how it applies to our daily lives. Each year from September through May, several groups gather on a weekly basis using Catholic Scripture Study International's acclaimed and doctrinally sound program for an in-depth study of one or more books of the Bible. The next session begins in September 2016. Sessions meet at one of two times each week:
- Tuesdays 1-2:30pm
- Wednesdays 7-8:30pm
Registration and more information will be available in August. There is a fee of $65 helps to cover the cost of materials. Please email Danette Morris with your name, contact info (phone and preferred email), and which time option you prefer.
Each session meets for 1 1/2 hours a week to discuss the current reading, share answers to a set of study questions and view a DVD featuring a theologian who provides the groups gathered with deeper insight into the passages just studied. These studies are suitable for all adults from beginner through advanced.
We also offer short (4-6 week) studies several times per year especially during the penitential seasons of Advent and Lent. Information about each study is posted in the bulletin at least 4-6 weeks prior to its start.


text:
Defying Evil, and Mortality
Published: August 1, 1996
(Page 2 of 2)
One enters her house through twin sitting areas with mirrored walls and bookcases. Ms. Giovanni lives with a friend, Virginia C. Fowler, an English professor at Virginia Tech, and Wendy, 5, a Cairn terrier.
At one point, as Ms. Giovanni sits talking, Ms. Fowler steps out to have a pensive smoke on the screened-in back porch. She wrote the introduction to Ms. Giovanni's selected poetry and has written a book about her. She writes often of black women writers.
The women get along well, Ms. Giovanni says, acknowledging that she is not the easiest housemate, for like most writers, she says, she lives too much inside her own head.
Ms. Giovanni is working on a book of love poems. She is also wrestling with a book about her cancer, which she has titled ''A Deer in Headlights.'' Ms. Giovanni expects it will take a year or so to finish.
''Last year, I had a very productive year,'' Ms. Giovanni says. ''I'm really not going to put myself on a deadline. The tendency with writers is always to say, 'What's the next thing?' I don't have any hard deadlines.
''I want to enjoy what I've done. You get sick of that 'you've got to top yourself.' I don't want to be on that track.''
Her eyes well with tears as she remembers the support of her students when she told them she might be too sick to finish their writing course, or might even be dead. Ms. Giovanni teaches an introductory and an advanced course in creative writing and a course on the Harlem Renaissance.
''I said: 'I have been diagnosed with cancer. I may or may not survive. Even if I do, you're not going to get my attention. If you want to transfer out of this class, you probably should. If you want to stay, I'll do the best I can.' '' She pauses, her voice drops, then cracks. ''I would have left. Not a one did. If ever I loved a class, I loved them. Because they didn't have to do it.''
She is trying to teach her students to think like writers, which, she says, means getting into the heads of other people -- telling the story of a people in poetry, rather than leaving it to sociologists or historians.
''I think black people are fantastic,'' she says. ''Any other people, knowing that tomorrow doesn't hold anything, would despair.''
And she dismisses the notion that the current crop of young people are apolitical at best or nihilistic at worst.
''I have a lot of respect for this generation,'' she says. ''It's a cheap shot to say we did so much more than them. In some ways, the struggle is more difficult now. I'd rather take what we did -- if we were killed or beaten, you knew you were fighting the system. These young people leave their house, and people can just decide they don't like the way you look and kill you.''
The battle is more difficult now, she says, because the racism is more sophisticated and insidious than segregated drinking fountains.
Ms. Giovanni believes the racism of everyday life in the United States is comparable to the complicity of ordinary Germans in the oppression and murder of Jews in Nazi Germany.
''You can't have a racist cop up in L.A., a racist cop in Cincinnati, a racist cop in Philadelphia and not see a systemic pattern of brutality,'' Ms. Giovanni says. ''These are not isolated incidents.''
Still, even for her, not everything is so deep. There are the simple pleasures of being home, of grilling on the back deck and watching the birds. Ms. Giovanni is a sports fanatic, enjoying anything where she can watch a ball. She enjoys spending time with kids and old people, science fiction, watching the Weather Channel. She is Southern enough to apologize that a delicious homemade poundcake is two days old.
''To me, God is in his heaven and all is right with the world if you have a nice red or white potato with butter,'' Ms. Giovanni says.
''I think people think life is some game you're playing,'' she says. ''The life you're leading is your life -- it's no 'Wait until I graduate.' Whatever you are here and now, it's what you are.''
Photo: Poet and her dog: Nikki Giovanni and Wendy, in Virginia. (Cindy Pinkston for The New York Times)


text:
Greg LittleWR BUF (10) 6 months ago
Little was signed as a free agent Tuesday, BuffaloBills.com reports.
EDGE Analysis
Little has had three 40-plus catch seasons since getting drafted in the second round by the Browns in 2011, but has fallen on hard times recently, with just six catches in 2014 while not seeing any action this past season. He'll compete for a spot for a fourth, fifth, or sixth wideout in camp this summer.
Greg LittleWR BUF (10) 10 months ago
Little is inactive for Sunday's game against the Ravens.
EDGE Analysis
Little's inability to play on special teams has prevented him from earning an appointment to the active roster for any of the Bengals' three games this season. Expect Little to enter the lineup only if one of the team's top four receivers goes down with an injury.
Greg LittleWR BUF (10) 10 months ago
Little is inactive for the Bengals' season opener against the Raiders, Paul Dehner Jr. of the Cincinnati Enquirer reports.
EDGE Analysis
Little was able to claim a spot as the Bengals' No. 5 receiver in training camp, but because of his limited ability to contribute on special teams, he'll be in street clothes for the opener.
Greg LittleWR BUF (10) 11 months ago1 Comment
Little surprisingly won a roster spot with the Bengals, due in part to his ability to play on special teams.
EDGE Analysis
Little isn't guaranteed to be dressed for Week 1 - he essentially will battle week-in, week-out with rookie Mario Alford.
Greg LittleWR BUF (10) 1 year ago
Little was released by the Bengals on Friday.
A former second-round selection of the Browns, Little has struggled with drops throughout his career, along with an inability to consistently get separation. He does offer an excellent combination of size and strength for a wide receiver, so he shouldn't have much trouble finding a team that will let him compete for a roster spot. At this point, it's probably safe to write Little off as nothing more than a depth player.
Greg LittleWR BUF (10) 2 years ago
Little (illness) is inactive for Sunday's game at Tampa Bay, Coley Harvey of ESPN.com reports.
An illness is excuse enough to deem Little inactive for the third week in a row, and eighth time in 12 contests this season.
Greg LittleWR BUF (10) 2 years ago
Little is inactive for Sunday's game against the Texans.
That's back-to-back games as a healthy inactive for Little, who has clearly fallen to the bottom of the Bengals' wide receiver depth chart.
Greg LittleWR BUF (10) 2 years ago
Little is inactive for Sunday's game against the Saints.
As a healthy scratch Sunday, Little remains a break or two away from fantasy relevance.
Greg LittleWR BUF (10) 2 years ago
Little had three catches for 42 yards in the Bengals' win over the Ravens on Sunday. Two of those catches came in the red zone, getting the Bengals inside the 5-yard line.
Little actually was open in the end zone on another target, but Andy Dalton missed him. Little's action could decline, however, when A.J. Green returns, possibly as early as Week 9.
Greg LittleWR BUF (10) 2 years ago
Little signed with the Bengals on Tuesday, NFL.com reports.
Little has been released by both the Browns and Raiders this season, but now joins the Bengals to help their situation at wide receiver. With both A.J. Green (toe) and Marvin Jones (ankle) slated to miss Sunday's game versus the Colts, Little's signing adds depth to Cincinnati's wideout options. While he's likely to be buried behind Mohamed Sanu, Brandon Tate, Dane Sanzenbacher and James Wright in the Bengals' pecking order at the position, Little now has the chance to catch-on with another franchise.


text:
Industry News
Intersil Corporation, a leading provider of innovative power management and precision analog solutions, today announced financial results for the second quarter ended July 1, 2016. Second quarter revenue of $134.0 million was up 3.7% sequentially and 1.2% year-over-year. Both Consumer and Computing and Industrial and Infrastructure revenue were up year-over-year. I&I demand improved broadly, resulting in 7.8% sequential growth...."/>
PR Newswire - 07/28/2016 4:05 PM ET
MicroStrategy ® Incorporated, a leading worldwide provider of enterprise software platforms, today announced financial results for the three-month period ended June 30, 2016. Second quarter 2016 revenues were $123.1 million versus $132.9 million for the second quarter of 2015, a 7% decrease. Product licenses and subscription services revenues for the second quarter of 2016 were $31.2 million versus $36.4 million for the second..."/>
PR Newswire - 07/28/2016 4:05 PM ET
A10 Networks, Inc., a technology leader in application networking and security, today announced financial results for its second quarter ended June 30, 2016.. Second Quarter 2016 Financial Highlights. Record revenue of $57.1 million, up 20 percent year-over-year Enterprise revenue of $32.0 million, increased 16 percent year-over-year Product revenue of $38.8 million, up 16 percent year-over-year Cash and marketable securities increased to $113.7..."/>
Business Wire - 07/28/2016 4:03 PM ET
Power Integrations today announced financial results for the quarter ended June 30, 2016. Net revenues for the second quarter were $97.2 million, up 14 percent from the prior quarter and also up 14 percent compared to the second quarter of 2015."/>
Business Wire - 07/28/2016 4:02 PM ET
Microsemi Corporation, a leading provider of semiconductor solutions differentiated by power, security, reliability and performance, today reported unaudited results for its third quarter of fiscal year 2016 ended July 3, 2016.. Net sales for Microsemi's third quarter of fiscal year 2016 were $431.4 million, up 36.0 percent from $317.1 million in the third quarter of 2015.. GAAP gross margin for the third quarter of 2016 was a record..."/>
PR Newswire - 07/28/2016 4:02 PM ET
NexJ Systems Inc., a provider of enterprise customer management solutions to the financial services industry, today announced financial results for its second quarter ended June 30, 2016.. $15.1 M in revenue for the six month period represents 13% growth over the same period in the prior year $396,000 in EBITDA for the six month period is a $1.3 M improvement over the EBITDA loss of $911,000 for the comparative period $11 M cash balance at the end of the..."/>
Canada Newswire - 07/28/2016 4:01 PM ET
Brooks Automation, Inc., a leading worldwide provider of automation and cryogenic solutions for multiple markets including semiconductor manufacturing and life sciences, today reported financial results for the third fiscal quarter of 2016, ended June 30, 2016.. Fiscal Third Quarter of 2016 Financial and Operational Highlights:. Revenue was $147.5 million; GAAP Net Income was $8.6 million with diluted EPS of $0.12; Non-GAAP Net Income was..."/>
GlobeNewswire - 07/28/2016 4:01 PM ET
Intuit Inc. will announce its fourth-quarter and full fiscal year 2016 financial results on Aug. 23 following the close of market. The company' s fiscal year ends on July 31.. Intuit executives will discuss the financial results on a conference call at 1:30 p.m. Pacific time on Aug. 23."/>
Business Wire - 07/28/2016 4:00 PM ET
Xplore Technologies Corp., a manufacturer of fully-rugged and ultra-rugged tablet PCs, announced that it will release financial results for its fiscal 2017 first quarter, which ended June 30, 2016, on Wednesday, August 10, 2016 after the market close. The company will subsequently conduct a conference call and webcast to review its results at 4:30 p.m. ET.. Interested parties in the United States can access the call by dialing 877-269-7756;..."/>
GlobeNewswire - 07/28/2016 4:00 PM ET
Sunworks, Inc., a provider of solar power solutions, announced today that it will report its second quarter 2016 financial results before the market opens on Wednesday, August 10, 2016.. Management will host a conference call to discuss these results on Wednesday, August 10, at 10:00 a.m. ET. To access the call, please dial 1-800-901-2731 or 1-785-424-1096. The conference call will also be broadcast live over the Internet, which can be accessed via the..."/>
Marketwire - 07/28/2016 4:00 PM ET
PDF Solutions, Inc., the leading provider of process-design integration technologies to enhance integrated circuit manufacturability, today announced financial results for its second fiscal quarter ended June 30, 2016.. Total revenues for the second fiscal quarter of 2016 totaled $26.7 million, up 6% from $25.1 million for the first fiscal quarter of 2016 and up 15% from $23.2 million for the second fiscal quarter of 2015. ..."/>
GlobeNewswire - 07/28/2016 4:00 PM ET
CyberOptics Corporation today reported operating results for the second quarter of 2016 ended June 30.. Second quarter sales increased 82% to $18.6 million from $10.3 million in the second quarter of 2015. Earnings totaled $2.0 million or $0.29 per diluted share, compared to the loss of $761,000 or $0.11 per share in the year-earlier period.. Subodh Kulkarni, president and chief executive officer, commented:" The continuation of strong operating results in this..."/>
Business Wire - 07/28/2016 4:00 PM ET
Business Wire - 07/28/2016 4:00 PM ET
Grubhub shares popped about 25 percent on Thursday following a quarterly report that surpassed analyst estimates on revenue and earnings per share. The Chicago-based food ordering and delivery company posted revenue of $120.2 million, topping analyst expectations of $113.7 million. That's up 37 percent compared to the second quarter of 2015, when Grubhub revenue was $88 million."/>
McClatchy-Tribune - 07/28/2016 3:52 PM ET
McClatchy-Tribune - 07/28/2016 3:51 PM ET
McClatchy-Tribune - 07/28/2016 3:51 PM ET
NetSuite Inc. by
Oracle Corporation. On July 28, 2016, the two companies announced the signing of a definitive merger agreement pursuant to which Oracle will acquire NetSuite. Under the terms of the agreement, NetSuite shareholders will receive $109.00 for each share of NetSuite common stock."/>
Robbins Arroyo LLP: Acquisition of NetSuite Inc. (N) by Oracle (ORCL) May Not Be in Shareholders' Best Interests
Business Wire - 07/28/2016 3:41 PM ET
Systems Ltd. finished the second quarter with a 9.1% increase in non-GAAP revenue to $235.4 million and a $47.9 million non-GAAP net profit, compared with $39.5 million in the second quarter of 2015. Fully diluted non-GAAP net profit per share reached $0.79, 23% more than in the corresponding quarter last year."/>
McClatchy-Tribune - 07/28/2016 3:35 PM ET
GlobeNewswire - 07/28/2016 3:19 PM ET
BioTrackTHC and Cannabase Announce Integration Partnership Streamlining the Cannabis Wholesale Market with Robust Live Inventory Capabilities
PR Newswire - 07/28/2016 3:17 PM ET
McClatchy-Tribune - 07/28/2016 2:43 PM ET
Ixia to Showcase Solutions that Deliver Better Visibility and Smarter Security at Black Hat USA 2016
Business Wire - 07/28/2016 2:29 PM ET
Texas Instruments Incorporated Chairman, President and Chief Executive Officer Rich Templeton will deliver keynote remarks at the Pacific Crest 18 th Annual Global Technology Leadership Forum in Vail, Colo., on Monday, August 8, at 12:05 p.m. Mountain time. Templeton will field questions from analysts and investors, as well as discuss TI's business outlook and its strategy to address key markets for its analog and embedded processing..."/>
PR Newswire - 07/28/2016 2:15 PM ET
Mack Trucks using virtual reality to show off automated manual transmission [The Morning Call (Allentown, Pa.)]
McClatchy-Tribune - 07/28/2016 2:11 PM ET
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Toronto, ONCanada
April 18, 2014
Medium length weekday rides (evenings) and longer weekend day trips. Also, social rides.
Hi everyone! Just bought a new bike, excited to get out on the trails and meet new likeminded people.
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Ravell Call, Deseret News
Seniors are standing on the edge of the upcoming fiscal cliff, according to U.S. News.
While decisions in legislation will determine how much this affects individuals, here are six ways that it can affect seniors in particular, according to the U.S. News article.
Increased dividend taxes
Income received from stock and mutual fund investments are taxed at 15 percent currently, but if we go over the fiscal cliff, they will be taxed at ordinary income rates, which will reach up to 40 percent.
Increased taxes on capital gains
For investments held longer than a year, which are long-term capital gains, the tax rate will increase from 15 to 20 percent.
Social Security
Actually, the fiscal cliff could bring somewhat good news for Social Security. The benefit would not get higher, but the 2 percent payroll tax break that was established in 2010 would end. The payroll tax funds Social Security, so that tax could repair some damages that happened in the program funding.
Medicare cutbacks
There would be $11 billion cut out of Medicare, which would lower the payments made to doctors. Patients may potentially have to pay the difference. Because of this, doctors could also limit the number of Medicare patients they would see.
Value of homes
Mortgage rates are a big guessing game. It's unlikely for home prices to rebound with less government spending, less employment and less after-tax income. It could cause home values to drop again.
Investment value
If the economy suffers after going over the fiscal cliff, then investment returns would be lower. When that happens, lower returns are taxed at a higher rate. This could cause high-dividend stocks, defense stocks and banking stocks to take big losses.
EMAIL: email@example.com
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- Get all your outdoors work done by... 12
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The Spital Inn And The Hand of Glory
A Hand of Glory was used by a gang of thieves in an attempt to rob the long gone Spital Inn, North Stainmore in 1797. Read More »
Spofforth Castle
A holding of William de Percy, one of the early supporters of William the Conquer, who was given vast tracts of land in Yorkshire for his brave service. Read More »
The Spriggans
Spriggans is the name given to a family of fairies in Cornish folklore, they are the closely related to the Piskies, but were generally believed to be darker and more dangerous than their mischievous cousins. Whereas Piskies are generally described as being cheerful and fun loving, Spriggans are more spiteful and full of malice, directed at humans in the form of evil tricks. Read More »
Spring Cottage, Bloxwich
The present day Spring Cottage dates back to the turn of the 20th century and was built on the site of a former pub. The former pub on the site was reputedly used as a temporary gaol for prisoners in Victorian times. They would be incarcerated in the cellar where there was no chance of escape. Read More »
Spring Heeled Jack – The Terror of the Black Country
Out of the dark, supernatural depths of Victorian England one name stands out. Jack.
Not Jack the Ripper, but a more supernatural fiend - Spring Heeled Jack! Read More »
Spring Heeled Jack? Ewell 2012
The following story by Lauren May entitled 'Paranormal experts weigh in on dark figure mystery' appeared in the Epsom Guardian 8 March 2012 and concerns a strange experience reported by multiple witnesses that took place on Valentines Day. Read More »
SS Great Britain
The SS Great Britain ranks amongst the most famous ships every built. Over 160 years old she now rests in same the dry dock that was specially created for her construction in Bristol harbour. The dock itself is now airtight and environmentally controlled to preserve the mighty vessel and prevent her wrought iron hull from being eaten by corrosion. Read More »
St Albans Abbey
St Albans has a multitude of ghosts and strange stories, many of which are attached to the magnificent abbey. St Albans has been occupied from very early in its history, the Roman town of Verulamium once stood in the valley, in the area where the public park now lies. Read More »
St Alkelda's Well, Middleham
St Alkeda was a chaste Saxon maiden, sometimes described as a princess, noble woman or a nun. On 28th March 800AD, somewhere close to the site of St Mary's and St Alkelda's Church, she was strangled to death for her faith by two Danish women involved in a Viking raid. It has been suggested that they killed her by twisting a napkin around her neck. Read More »
St Andrew's Churchyard, Rodney Street
A pyramid monument to the William McKenzie (20 March 1794-29 October 1851) rests in the churchyard of the (currently disused and needing restoration) Scottish Presbyterian Church of St Andrew's, dating from 1824. McKenzie made his fortune as a civil engineer in the Victorian era but it is the nature of his burial, or lack of it that has become legendary. Read More »
St Andrew's Parish Church, Alfriston
St Andrew's Parish Church is a Grade I listed building dating back to 1370. It was built in a cruciform shape and is referred to as The Cathedral of the Downs. There is a siting legend attached to St Andrews Church dating back to its original construction. Read More »
St Andrews Church, Cobham
St Andrews in Cobham dates back to the 12th century, though it has been through extensive renovation during its 800 year history. The church is supposed to be haunted by a strange apparition, that of a blue donkey.
St Andrew's Church, Banwell
The following article entitled 'The Glory of Banwell Church', edited by Jill Bailey, was originally published on 28 September 1963 and republished in the The Weston Mercury on 23 May 2008. Read More »
St Anne's Castle, Great Leighs
St Anne's Castle appeared in the Domesday Book (1086) and is one of the oldest pubs in the United Kingdom, if not the oldest. It is reputed to have a haunted room and poltergeist activity has been experienced in the past. Read More »
St Audries Black Dog
In 'Unexplained Phenomena: A Rough Guide Special' (2000) by John Michell, Bob Rickard and Robert J M Rickard, refer to a Black Dog that is thought to have haunted the road between St Audries and Perry Farm. They quoted their source as the Somerset Volume of County Folklore. The Dog is thought to have been witnessed by two people in 1960 shortly before their deaths.
St Augustines Well, Cerne Abbas
All the following details were made available on the information board inside the burial ground situated above St Augustine's Well; Read More »
St Bartholomew's Church, Fingest
St Bartholomews Church is a Grade I listed building and dates back to the 12th century. There is a folk good luck custom associated with weddings at St Batholomews, where the groom is expected to lift his bride over the church gate after the ceremony. To ensure this is done the church gate is usually kept locked on such occasions. Read More »
St Batholomew the Great Parish Church
Founded in 1123 by Rahere, a jester/minstrel in the court of King Henry I (1068 – 1 December 1135), making this one of the oldest churches in London. Originally established as an Augustinian Priory Church, its nave was demolished in 1539 when King Henry VIII ordered the Dissolution of the Monastery's. Read More »
St Bees Priory
The atmospheric church at St Bees is all that remains of a small Benedictine monastery closed down during the reformation. The priory is associated with the legend of St Bega, who is said to have fled here to escape an arranged marriage in Ireland. Read More »
St Botolph's Church without Bishopgate
In 1982 Chris Brackley took a famous photograph whilst he was in St Botolph's Church. The photograph was of the interior of the church, taking in the aisle, altar and main stain glass window. In the upper right hand side of picture there appears to be ghostly image of a figure dressed in period costume in the Choir Loft. Read More »
St Catherine's Church, Barmby Moor
Dating from around 1272, St Catherine's Parish Church was largely rebuilt in 1850 replacing much of the original Norman building. In the churchyard, just south of the main door is a stone which has been speculated may have been a place of pagan worship. Read More »
St Chad's Church, Saddleworth
Saddleworth church - dedicated to St Chad - has a legend associated with its location. It is said that the original site for the church was on nearby Brown Hill, but every night the stones were mysteriously moved to their present position. Eventually the builders gave up moving the stones back to Brown Hill, and built it where the stones were placed each night. Read More »
St Cuthbert's Churchyard, Carlisle
The graveyard of this old church was the scene of grave robbing, along with other sites in Carlisle during the 1820s.
St Ethelbert's Holy Well
Around 794AD, King Offa of Mercia demanded the head of the Christian King Ethelbert of East Anglia whilst he was making arrangements to marry Offa's daughter. Not far from the location of Marden Church the young king was assassinated and his body hidden. After rumours of Ethelbert's ghost being seen in the marden area, Offa asked the Pope for absolution. Read More »
St George's Day
Today St George's Day is not celebrated in England with anywhere near the vigour it was in past centuries, and is actually celebrated more in other countries that share his patronage, with traditions that have not been broken for hundreds of years. Read More »


text:
Watervliet, MI 49098
Jean LaFever specializes in Physician Assistant (PA) and practices in Hartford, MI. She practices at 1 location.
Are you Jean LaFever? Add your own statement here.
For the best healthcare for your needs, choose a doctor who specializes in your medical condition.
Check to see that this provider treats your medical condition.
Check to see that this provider performs the procedure that you need.
Check to see if your provider has any malpractices, board actions, or sanctions.
Learn more about where this provider went to medical school, residency, and more.
Check to see what languages this provider and/or staff speak.
Likelihood of recommending Jean LaFever to family and friends is 3.8 out of 5
Check out the quality of care at the 1 hospitals where Jean LaFever has admitting privileges.
More Physician Assistants like Jean LaFever at Stagg Medical Center


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LOOK: "Supergirl" Introduces Superman in First Family Photo
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The new digital-first Marvel comic will pair the Sorcerer Supreme with the ruthless vigilante in a story by John Barber.
Google Play is offering a sale on Marvel and DC books during SDCC to promote the new easy-reading Bubble Zoom feature.
The digital comics service is adding a handful of major indie titles -- but only while Comic-Con International is running.
The latest installment of the "Overwatch" digital comic introduces Ana Amari, the newest hero of Blizzard Entertainment's hit game.
Digital comic sales dipped by 10% in 2015, New York Comic Con events will kick off on Oct. 3, and more!
Keiynan Lonsdale dons the iconic gold-and-red Kid Flash costume in the latest photo for "The Flash" Season 3.
Interact with the stories behind the stories as CBR's Instagram takes you behind-the-scenes, offering shots you can't find anywhere else.
Four older manga series are making a comeback in digital form, comic pro reactions to Brexit continue, and more.
The mobile comics subscription service says goodbye to one of its two founding members just months after launch.
The "all you can eat" digital comics service expands; Svetlana Chmakova's "Awkward" wins the second Dwayne McDuffie Award; and more!
Was Lee Bermejo's Joker really based on Heath Ledger? And did Grant Morrison almost create a female Doctor for "Doctor Who?"
This alternate take on Hanna -Barbera's cult hit cartoon is unlike anything else on the comic stand today, in all the right ways.
The famous Grumpy Cat is getting her own web series comic strip featuring her "misadventures" with her sidekick Pokey.
Now that comiXology's offering a Netflix-like service for countless titles in its library, will you be subscribing?
CBR spoke to "2 Broke Girls" star Beth Behrs about co-writing the comic "Dents," set in an apocalyptic world where the polar ice caps have melted.
The digital comic will explain how this new Inhuman essentially causes the Marvel Universe's latest superhero conflict.
Acclaimed cartoonist Dean Haspiel discusses his new webcomic about a super thief turned reluctant hero living in a sentient, heartbroken Brooklyn.
Koyama Press profiled! Go inside the Art of EC Comics! Interviews with Tom Smith and Joe Simon's granddaughter Megan Margulies!
Animation veteran Eddie Pittman tells CBR about his popular webcomic "Red's Planet" arriving to print this month and what he learned in the process.
Mary Jane enters the fray, Iron Man learns the truth about his parents, "Apocalypse Wars" concludes and more!
New chapters of Hajime Isayama's hit manga, and 16 other Kodansha titles, will debut on comiXology and Kindle the same day they're released in Japan.
In July, Jody Houser, Pere Perez and Marguerite Sauvage will kick off "Faith's" first ongoing series at Valiant Entertainment.
The 10,700-square-foot flagship store will be located in the Disneytown entertainment district, just next door to the new theme park.
DC Comics is releasing their digital-first "Adventures of Supergirl" series in print, twice-monthly, starting in May.
"Insufferable: On the Road" writer and Thrillbent co-founder Mark Waid discusses his story and working in all aspects of the comic book industry.
Before the Mighty Morphin heroes return tomorrow, check out 16 new takes on the Green Ranger and gang.
Want to talk about DC's "Rebirth," your favorite new book or what you had for lunch? It's all fair game on the CBR Community.
Indie vets Evan Dorkin & Sarah Dyer tell CBR about their new Stēla series with Erin Humiston, H.P. Lovecraft's influence and comics on your phone.
Beth Behrs, one of the leads from CBS' hit sitcom "2 Broke Girls," has written a new comic with her best friend Matt Doyle, titled "Dents."
The company is growing its "Batman: The Animated Series" offerings, launch several Harley Quinn-based lines and Intrdouce more Greg Capullo figures.
Charles Soule tells CBR about "Daredevil/Punisher," why the characters are at odds and why Daredevil's new partner Blindspot gets involved.
Saul Goodman goes full superhero in AMC's "Better Call Saul" comic by show writers Jenn Carroll & Gordon Smith and artist Steve Ellis.
The new weekly "Gotham Stories" motion comic will tell an original, five-part story that will bridge both halves of "Gotham" season two.
Marc Andreyko & Richard Ortiz's DC Comics series returns on Monday Feb. 1 with Clayface hitching a ride on Wonder Woman's invisible plane.
The writer tells CBR about his return to "Supergirl" with a new comic based on the CBS series influenced by "Hush" and Hayao Miyazaki films.
The new publisher's entire line of titles is now available digitally as well as in print.
Chris Onstad's beloved "Achewood" webcomic made a surprise return on Christmas Eve, along with another installment on New Year's Day.
CSBG has a library of notable retcons, like whether Captain America's best pal Bucky Barnes was a teen sidekick or a trained killer.
Writer Fabian Nicieza answers your questions about Deadpool, Cable, time travel and chimichangas.
The bi-weekly digital first series will also feature artwork from Jonboy Meyers, Emanuela Lupacchino, and Emma Vieceli
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