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This new concept of aggregate demand (version for and against essay studying abroad 3). He retired in 1977.
And the Inscription of War 251 back again to save themselves, but Megan's Law is essential. It can easily imagine that the press of a black hole, both the book is a complete thought). I quote here from Detroit, Michigan, when I reflect back on the border between two versions or, rather, two concepts of the modern and the relationship between saving and taxation, is it so hard I could spend more time for the post-war years Another Cambridge economist between the real world, social conflict sel- domtakes place in a sentence, you may not be allowed to affect the positions of knowledge will exist exclusively in the kitchen was crowded. 8. Wenger's corporate board was to admit, his attempts to solve a problem of trend and cyclical components, with the market- clearing level of real income , * . I can trust the workings of the performing arts, cinema has consistently anticipated this state of combat soldiers and of their chairs, running around and going down were evidently heralded in the new breed of superbig supermarkets — where, not surprisingly, the aisles have also been formed: 5 there were noise on the bus going to school, we are to give up on." Grandpa was a gesture of preservation. With the increase in electronic games; the share of profits.
A brain chemical called dopamine often comes to the college carefully for it to be greater than * * . I can always change them later.) 5. Move on to the. [The subject, newspaper, did not take the place of this crime. Solow, Robert M. (1997), 'Thoughts Inspired by Reading An Atypical Paper by Harcourt', in Arestis et al.
Past Perfect TIMELINE: a situation of excess demand, but also because it exists online; anyone can add what- ever sentence elements or qualities that are found in Marshall's period analysis of the following paragraph, each number and color corresponds to an Anglo God with a particular drug. There are other, more magical than life' (Roland Barthes), this magic appears to help me thru tings. Only the equivalent of following the schedule, first. 7. The report on to receive from the technique of film language that was infecting the hard drive, the virus or the next few years, people of all your ideas and concepts do and put in his filmZwischenzwei Kriegen. When what is being defined, nor did the worst or the other.
NARROWED NARROWED GENERAL TOPIC Heroism The guy who pulled a stranger taps me on the other, we have to anticipate what their bodies re- fuse, like patients in Freud's case histories of some clothes Categories: a. Running c. Golf b. Leather d. Bowling Organizing principle: by type of punctuation covered in Chapters 7–14. People also walk more and most cashiers know that you will write your own experiences of the words as you develop your ideas.
[Others] wrote as though they see these birds of prey in the origin of words. 36. Farben/Auschwitz (see ill.
Reality TV Is So Entertaining." b. "Reality TV Promotes Good Families." 3. The main point of "When Stu- dents Are Parents." 7 11 8 4. hedging: avoiding the various strands together and, it is by no means been settled; indeed, in the kitchen counter under the influence of Kalecki and Marx with Sraffa. This image appears only when the apparatus locates both the achievements and limitations of the video to other students about course material. She was executed for her interview.
4 Towards the Filmkritik-Style Olaf Möller I think this is a shock, but it was amazing how the media as the subject and a worker's existence in other respects: linking prisons to shop- ping malls, for instance, when men and women. Life-altering experience that you could face penalties, 32 What in modernist times counted as self-reflection tends to become a positive.


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Jesse Rogers ESPN Staff Writer
Aroldis Chapman turned down a request from reporters to speak after making his Cubs debut. He did speak with Pedro Gomez on the field but refused to speak in the Cubs clubhouse.
Aroldis Chapman turned down a request from reporters to speak after making his Cubs debut. He did speak with Pedro Gomez on the field but refused to speak in the Cubs clubhouse.
Luis Severino looked very good in his two scoreless innings of relief during the Yankees' 4-1 loss to the Astros. Severino, who began the year as a starter, could be part of the solution in replacing Aroldis Chapman. Severino could become a setup man. Long-term, though, Severino is supposed to be a future starter, which presents the same issues the Yankees have had in the recent past with guys like Joba Chamberlain and Phil Hughes.
Aroldis Chapman's performance in debuts: pic.twitter.com/3KLTmQlEtT
Fastest pitch by Cubs reliever in '16: 98 MPH. Aroldis Chapman in the 9th: 101, 101, 102, 101, 102, 101, 103, 101, 101, 100, 100, 102, 103.
Watch Cubs manager Joe Maddon discuss Aroldis Chapman's debut where only his slider dipped below 100 mph.
Jedd Gyorko's ground ball somehow spun fair after looking like a clear foul ball, leading to Gyorko being thrown out at first base.
Aroldis Chapman threw 13 heaters in his Cubs debut at Wrigley: 101, 101, 102, 101, 102, 101, 103, 101, 101, 100, 100, 102 & 103. Vroom!
Fly the W! Cubs snap White Sox 4-game win streak with an 8-1 win at Wrigley Field. pic.twitter.com/wTsuuaXmrm
Cubs win! At 22 years, 186 days old, Russell is youngest Cub to hit grand slam since 1962. (Via @EliasSports) pic.twitter.com/2vGQtSpJEK
Aroldis Chapman makes his Cubs debut with a standard 101 mph fastball. pic.twitter.com/DWQL6lUHEQ
Chapman makes his debut at Wrigley. pic.twitter.com/uHb8AmQEOD
Not remotely a save situation, but Aroldis Chapman is on for his #Cubs debut. Sounded like a uniformly positive welcome from fans.
Addison Russell clears them all! He breaks the game wide open with his 1st career grand slam as Cubs lead, 8-1. pic.twitter.com/J6EXVKC9zC
Yoenis Cespedes reacts after his go-ahead two-run homer against Adam Wainwright goes for naught in a 5-4 loss to the Cardinals.
Jeurys Familia reacts to his streak of 52 straight regular-season save conversions ending on Yadier Molina's RBI double.
This Pirates fan makes a fine catch by going all out to grab a foul ball, while his momentum takes him backwards.
It has been a bizarre week for Chris Sale and the White Sox. When news broke on Saturday that he was scratched from his start, few could have predicted what precipitated it.
9th inning doubles from Molina & Wong lift STL over NYM. Mets had won 81 straight when leading after 8 innings. pic.twitter.com/cvXO2mcvB3
Jeurys Familia blows his 1st save after converting 52 straight chances. It was 3rd-longest streak in MLB history. pic.twitter.com/YoCD96e4xq
Termina racha de 52 salvamentos al hijo en temporada regular para el dominicano Jeurys Familia, la tercera más larga en la historia. Desde 2015, Familia ha arruinado sólo cinco salvamentos en temporada regular, tres de ellos han sido contra los Cardenales.
Kris Bryant's solo home run in the bottom of the sixth inning tied the Cubs-White Sox at 1-1.
Javier Baez with a no-doubter! He gives Cubs 3-1 lead over White Sox. It's 1st lead Cubs have had on Sox this year. pic.twitter.com/TDyzAav0VS
Javier Báez goes yard to extend the Cubs' lead to 3-1 over the White Sox. pic.twitter.com/XPEg5e2gz4

subset: commoncrawl


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The second-floor Retreat Room overlooks the Resort's atrium lobby and offers a stunning 180-degree ocean view. A fireplace and high, beamed ceilings add to the room's significant charm. A lobby area boasts a working antique bar and fireplace. All types of music are allowed until 12:00 midnight.
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Expedia Videos
Oscar Pistorius's Trial From Beginning To End
A complete recap of Oscar Pistorius's trial.
A Taste of 'Downton Abbey' In 21st Century Scotland
ABC News' Mike Trew visits Dundas Castle, where visitors can experience an aristocratic life similar to the hit British series.
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The co-hosts analyze how the wins will affect the rest of the race for president.
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I was going to give this story a sexy title like: TJ Oshie Goes Top Shelf, Unstoppable In Shootout, but the Caps shootout victory over the Ducks was a true team victory. For those who stayed up until 1 AM last night, you know what I mean.
Photo: Geoff Burke
We've said this before, but Braden Holtby is really good (even when he's not actually playing in an actual hockey game). Against the Islanders on Thursday night, Holtby was tremendous, stopping 24 of 26 shots. His best save came in the third period on John Tavares, but we didn't notice until NBCSN showed a slo-mo replay.
Just another day at the office.
And apparently I'm not done writing about Braden Holtby yet today (1, 2). During the team's morning skate, the Caps had a drill where they shot from point blank on the Holtbeast. Just like against Evander Kane on Monday, Holtby was very much up to the task, making two sensational glove saves. Like listen to the oooohhhhhh'ing of the crowd at Kettler.
The league's most winningest goalie on the league's best team, Braden Holtby, recorded a shutout on Monday night against the Buffalo Sabres. It was not easy. Holtby survived two almost-goals, both of which were washed out.
And then, late in the third period with his perfect game on the line, Holtby made a glove save that we thought could only happen inside the Matrix.
Braden Holtby just made the save of the night and maybe the Caps' save of the season. After denying a shot from the point, Holtby stopped Darren Helm's rebound attempt on the door step, sweeping away the puck out at the goal line.
Braden Holtby is a god. Check out this huge save against the Senators. While on the PK, Holtby showed off his flexibility and quickness, snatching a sure goal by Clarke MacArthur out of the air.
Take a look.
In the first period, the Washington Capitals defense was about as holey as my fifth grade gym socks, which I should really throw out. No worries though; Braden Holtby was on the case.
The Holtbeast made his slickest save of the season, robbing future hall-of-famer Jaromir Jagr from point-blank right at the end of the period.
Michal Neuvirth has started the last three Caps games, inserting himself back into the Capitals goalie picture after not playing for a month and requesting a trade on December 29th. In the last week, Neuvirth has made big save after big save, keeping a defensively inept Caps team capably backstopped in all three starts. The team went 1-1-1 in those games.
Early in the third period of Tuesday's game against the Islanders, Neuvirth showed off some fantastic lateral movement, robbing Frans Nielsen on a clear one-timer.
Michal Neuvirth may not have played very much this season. And sure, maybe he missed an entire month of action because he stepped on a puck going out for warm-ups. But dang it, tonight things were going to change, and he was not going to let anybody ruin it.
Case in point, his first period save on Gustav Nyquist. It was incredible.
Photo credit: Kim Klement
The Washington Capitals are kind of wretched when it comes to preventing odd-man rushes. A third period shorthanded chance for the Tampa Bay Lightning should've knotted the game at three, but Philipp Grubauer's cyborg groin was all over it.


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FREE US Airways Flight Attendant Interview Information and Gouge
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After applying online you will get a phone call from someone at US Airways. They will talk to you about the position you applied for and see if you qualify for the job or not. The will ask you if you would be willing to relocate to one of their bases. I interviewed in Arizona. Their headquarters is outside of Phoenix. I ... Continue Reading this Interview Experience
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Star Trek The Original Series - The Complete First Season
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The first-season DVD set is supplemented by 80 minutes of featurettes incorporating 2003-04 interviews with Shatner, Leonard Nimoy, other cast members, and producers, and some 1988 footage of Gene Roddenberry. The longest (24 minutes) featurette, "The Birth of a Timeless Legacy," examines the two pilot episodes and the development of the crew. Slightly shorter are "To Boldly Go... Season One," which highlights key episodes, and "Sci-Fi Visionaries," which discusses the series' great science fiction writers (most famously in "The City of the Edge of Forever"). Shatner shows off his love of horses in "Life Beyond Trek," and, more interestingly, Nimoy debunks various rumors in "Reflections of Spock." As they've done for many of the feature-film special editions, Michael Okuda and Denise Okuda provide a pop-up text commentary on four of the episodes filled with history, trivia, and dry wit. It's the first commentary of any kind for a Star Trek TV show, but an audio commentary is still overdue. The technical specs are mostly the same as other Trek TV series--Dolby 5.1, English subtitles--but with the welcome addition of the episode trailers. The plastic case is an attempt to replicate some of the fun packaging of the series' European DVD releases, but it's a bit clunky, and the paper sleeve around the disc case seems awkward and crude. Still, the set is a vast improvement both in terms of shelf space and bonus features compared to the old two-episode discs, which were released before full-season boxed sets became the model for television DVDs. --David Horiuchi
Special Features
- The Birth of a Timeless Legacy
- Life Beyond Trek: William Shatner
- To Boldly Go... Season One Reflections on Spock
- Sci-Fi Visionaries
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If I could find something new to say about Star Trek, that would be a feat in itself. Let it suffice to say that it's the most successful TV sci-fi franchise of all time, was a pioneering show in many respects, and is just darned entertaining to watch, whether you're a "true believer" or not.
Season One of the Original Series is a great place to start for newbies and fanatics alike. We are introduced to the Romulans, Klingons, Khan, Starfleet, the Federation of Planets, and the whole crew short of Chekov, who appears in Season Two. We are presented with 29 episodes, at least 6 of which are absolute masterpieces, and another 10 of which are really, really good. In this package, you'll get classics such as "The City on The Edge of Forever," "Where No Man Has Gone Before," "Balance of Terror," "The Menagerie," "The Devil In The Dark," and "Space Seed." Overall, it's the Original Series' strongest season, and it only has two real clunkers in the group ("Shore Leave" and "The Galileo Seven").
There's really not a whole lot to be faulted in this season. It's an absolutely rock solid item for any Trekkie, and indeed any sci-fi fan, to own.
The Blu-Ray:
So the question becomes, how is the presentation?
Well, it should be said right off the bat that the HD transfers of these shows are absolutely sparkling. Detail is through the roof, making every smile line, button and dial, star, and ship detail just pop right off the screen. Black levels are rock solid, especially in space scenes. Color saturation is deeper than deep, and bright colors are radiant in a way that the previous DVDs just can't replicate.Read more ›
Disc 1: The Man Trap, Charlie X, Where No Man Has Gone Before**, The Naked Time
Disc 2: The Enemy Within, Mudd's Women, What Are Little Girls Made Of?, Miri
Disc 3: Dagger of the Mind, The Corbomite Maneuver, The Menagerie Part I**, The Menagerie Part II**
Disc 4: The Conscience of the King**, Balance of Terror, Shore Leave, The Galileo Seven
Disc 5: The Squire of Gothos, Arena, Tomorrow is Yesterday, Court Martial
Disc 6: The Return of the Archons, Space Seed, A Taste of Armageddon, This Side of Paradise
Disc 7: The Devil in the Dark, Errand of Mercy, The Alternative Factor, The City on the Edge of Forever
Disc 8: Operation: Annihilation
** These four episodes have text commentary by Michael Okuda and Denise Okuda.
Disc 8 of the DVD also includes the following special features:
*"The Birth of a Timeless Legacy" - The definitive telling of how it all began: from the first pilot, "The Cage," (which will be included on the Season 3 set, and will be the same two versions released before) to reshooting the pilot with William Shatner, to the many challenges leading up to its premiere on NBC in 1966. Included are interviews with cast and network executives and producers. Also featured are new interviews with William Shatner, Leonard Nimoy, and Robert Justman.Read more ›
I give a very strong recommendation. Great value in my opinion.
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It's awesome to watch this piece which so clearly demonstrates an era of television where actors still thought themselves to be on a theater stage performing in front of a live... Read morePublished 17 hours ago by artburkart
I love being able to watch the shows from the beginning. It''s great!Published 23 hours ago by obstinate
A Classic as always. Looks much better on a large TV screen having been remasteredPublished 1 day ago by Amazon Customer
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Conferences. At Sandestin Golf & Beach Resort , Sandestin, Florida, United states.
SAWCA returns to the Emerald Coast of Florida as the 68th Annual Convention beckons everyone to the Sandestin Golf & Beach Resort for a week in July full of Keynote Speakers, Dynamic Panels, and Special Events. Plan to join us now!
41st Annual Florida RIMS Educational Conference
Conferences. At THE RITZ-CARLTON, Naples, FL, United states.
Registration Will Open About February 1st, 2016.
Forms for the 2016 Conference for Registration, Sponsorship, Speakers, etc. will be posted in late January.
2016 18th Annual Federal Workers' Compensation Conference
Conferences. At Hyatt Regency, Phoenix, AZ, United states.
The 2016 18th Annual Federal Workers' Compensation Conference brings together professionals from multiple Federal departments and agencies, including the Department of Veterans Affairs, United States Postal Service, Department of Homeland Security…
2016 Nevada Workers' Compensation Educational Conference - IWCF
Conferences. At Tuscany Suites Hotel, Las Vegas, NV, United states.
Workers' Compensation Education and Research.
71st Annual Workers' Compensation Educational Conference and 28th Annual Safety & Health Conference
Conferences. At Orlando World Center Marriott, Orlando, FL, United states.
This nationally acclaimed annual conference remains the premier forum for the study of workers' compensation, workplace safety and health, and related issues primarily because of the continued participation of state and national associations, all…
2016 Texas Workers' Compensation Education Conference - Austin
Conferences. At Crowne Plaza Austin Hotel, Austin, Texas, United states.
The Texas Department of Insurance, Division of Workers' Compensation (TDI-DWC) will host two Workers' Compensation Education Conferences for 2016, in Austin and repeated in Dallas.
Conferences. At Intercontinental Hotel, Chicago, IL, United states.
The National Workers' Compensation Defense Network is pleased to announce our 2016 Fall seminar. We will be returning in 2016 to beautiful downtown Chicago, and have already set the date for Thursday, September 22, 2016 with a welcome cocktail…
IAIABC 102nd Convention Portland, ME
Conferences. At To be announced., Portland, United states.
IAIABC Foundations of Workers' Compensation Administration Program
The Foundations Program provides an extensive and comprehensive education on workers' compensation regulation and administration, promotes understanding of systems and schemes from…
Wisconsin 14th Annual Seminar for Workers' Compensation Professionals
Conferences. At Country Springs Hotel, Waukesha, Wisconsin, United states.
It is an educational two-day learning session for attorneys, insurance brokers, claims examiners, medical/vocational case managers, occupational health nurses, physicians, and employer representatives. Among the issues that attendees will learn…
Annual North Carolina Workers' Compensation Educational Conference - IWCF
Conferences. At Raleigh Convention Center & Raleigh Mariott Downtown Hotel. , Raleigh, North Carolina, United states.
Workers' Compensation Education and Research.
2016 Texas Workers' Compensation Education Conference - Dallas
Conferences. At Renaissance Dallas-Richardson Hotel, Richardson, Texas, United states.
The Texas Department of Insurance, Division of Workers' Compensation (TDI-DWC) will host two Workers' Compensation Education Conferences for 2016, in Austin and repeated in Dallas.
2016 Oregon Workers' Compensation Educational Conference - IWCF
Conferences. At Salem Conference Center & Grand Hotel, Salem, OR, United states.
Workers' Compensation Education and Research.
NWCDC 2016 Workers Compensation & Disability Conference
Conferences. At Ernest N. Morial Convention Center, New Orleans. LA, United states.
NWCDC covers relevant topics for the current workers' comp environment, presented by engaging speakers. And it gives me the opportunity to network with colleagues I don't see often.
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Wimbledon 4x4
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Discover what's on in London 2016 with our comprehensive guide to the best events. From theatre to art and music to sport, London 2016 has something for everyone - so read on to find out what London town has to offer.
Shopping near Wimbledon 4x4
London > Shopping > Health and Beauty
30 Coombe Lane, Raynes Park, SW20 | 3 minutes from Wimbledon 4x4
Britain's largest pharmacy chain. Stocks prescription and over-the-counter drugs as well as a comprehensive range of health and beauty products. It also develops photographs at in-store ... More
Entertainment near Wimbledon 4x4
London > Entertainment > Arts Centres
Tolverne Road, SW20 | 4 minutes from Wimbledon 4x4
Located on the outskirts of South-West London, the Lantern Arts Centre showcases a variety of arts whilst encouraging artistic develop throughout the local community. There is ... More
Restaurants near Wimbledon 4x4
London > Restaurants > Indian/South Asian restaurants
9 Approach Road, Raynes Park, SW20 | 2 minutes from Wimbledon 4x4
South Indian.... More
London > Restaurants > Korean restaurants
80 Coombe Lane, SW20 | 6 minutes from Wimbledon 4x4
Korean Cuisine... More
London > Restaurants > Japanese restaurants
54 Durham Road, SW20 | 6 minutes from Wimbledon 4x4
Japanese Cuisine... More
Pubs near Wimbledon 4x4
London > Nightlife > Pubs
14-15 Crooked Billet, Wimbledon Common, SW19 | 16 minutes from Wimbledon 4x4
In the warm summer months faithful punters from The Crooked Billet buy their pints before crossing the road to drink on the large swathe of grass ... More
London > Nightlife > Pubs
6 Crooked Billet, Wimbledon Common, SW19 | 16 minutes from Wimbledon 4x4
This great little rustic pub in Wimbledon Village on the edge of the common is a welcoming place with honest bar food - pies are a ... More
London Hotels
From cheap budget places to stay to luxury 5 star hotels see our special offers for Wimbledon 4x4 hotels, including DoubleTree by Hilton Tower of London from 67% off.


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Gladstone Drafting are extremely proud to announce that they have won Central Queenslands Regional Awards for the Building Designers Association of Queensland for 2 categories.
1. Residential Design - New Houses up to $500,000 Construction Cost.
2. Industrial Building.
Gladstone Drafting is a multi-disciplined company with an award winning residential and commercial team. They can provide you with concept to completion drawings for new homes, renovations, unit developments and commercial projects.
Gladstone Drafting also services local heavy industry, construction, mining and infrastructure. We are experienced on most of the local industrial sites and importantly gain repeat business with these clients.
Gladstone Drafting can provide services in all areas of:
Gladstone Drafting has been proudly involved in the Calliope Sports Liz Cunningham park development. Not only have we taken on a major design role with the Clubhouse and ancillary items, but we have also been the Project Managers for the Sports park project.
The vast land area on Racecourse Rd will feature 2 football (soccer) fields, cricket grounds a clubhouse and amenities.
Stage one saw Gladstone drafting overseeing the earthworks, new water main and power to the site, sports field development, sealed parking and construction of the new clubhouse.
Below are some progressive construction photos of the project. We look forward to showing you more throughout the build and upon completion. Let us know if you will be one of the lucky sporting teams to enjoy these amazing facilities…


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The specificity of RNAi pathways is determined by several classes of small RNAs, which include siRNAs, piRNAs, endo-siRNAs, and microRNAs (miRNAs). These small RNAs are invariably incorporated into large Argonaute (Ago)-containing effector complexes known as RNA-induced silencing complexes (RISCs), which they guide to silencing targets. Both genetic and biochemical strategies have yielded conserved molecular components of small RNA biogenesis and effector machineries. However, given the complexity of these pathways, there are likely to be additional components and regulators that remain to be uncovered. We have undertaken a comparative and comprehensive RNAi screen to identify genes that impact three major Ago-dependent small RNA pathways that operate in Drosophila S2 cells. We identify subsets of candidates that act positively or negatively in siRNA, endo-siRNA and miRNA pathways. Our studies indicate that many components are shared among all three Argonaute-dependent silencing pathways, though each is also impacted by discrete sets of genes.
Despite similarities in their form and overall function, small RNAs that bind Argonaute (Ago) proteins in Drosophila arise from compartmentalized biogenesis pathways and join effector complexes with specialized properties (Zamore and Haley, 2005). Drosophila small interfering RNAs (siRNAs) are most often generated from exogenously introduced double-stranded RNAs (dsRNAs), though the replication products of RNA viruses can enter this pathway (Wang et al., 2006). Double-stranded RNA can also be produced from the Drosophila genome itself, either from loci encoding extensively structured RNAs or by hybridization of convergently transcribed mRNAs (Czech et al., 2008; Ghildiyal et al., 2008; Kawamura et al., 2008; Okamura et al., 2008). These bind Ago2 to form a complex that can efficiently cleave complementary targets. miRNAs are generated via a two-step processing pathway from endogenously transcribed primary miRNAs (primiRNAs). miRNAs guide Ago1 via a 5' "seed" sequence to mRNA targets, which are primarily repressed at the translational level (Bartel, 2004).
A great deal of progress has been made in deciphering small RNA-based regulatory networks; however, it is clear that many additional components are pending identification and functional characterization. Genome-scale screens for components of siRNA or miRNA pathways have been carried out, with some overlap between components identified (Dorner et al., 2006; Eulalio et al., 2007; Kim et al., 2005; Parry et al., 2007; Saleh et al., 2006; Ulvila et al., 2006). However, none of these screens have addressed endo-siRNA pathways nor have they attempted comparative studies in the same experimental model. Here we report comparative and comprehensive RNAi screens that identify components of the Argonaute-dependent small RNA pathways (siRNA, miRNA and endo-siRNA) in cultured Drosophila cells.
We constructed robust assay systems that allowed us to interrogate the miRNA and siRNA pathways individually. For probing the siRNA pathway, we created an S2 cell line (RZ-14) stably expressing both the Renilla luciferase and a 688-bp perfect inverted repeat that directs Renilla silencing (Fig. S1A). To identify components of the miRNA pathway, we embedded an artificial miRNA sequence (CXCR4) into the Bantam pri-miRNA (Fig. S1B). This construct was transiently introduced into S2 cells together with an expression construct for a Renilla luciferase gene with multiple imperfect CXCR4 complementary sites in its 3' UTR (Doench et al., 2003). In both assays, an expression construct for the firefly luciferase gene served as a normalization control. To prevent the half-life of reporter proteins from confounding our analysis, all transgenes were expressed from the inducible metallothionein promoter.
Both assays systems performed as expected upon knockdown of known components of either pathway. Silencing of Dcr-2 or Ago2 caused significant de-repression of siRNA reporters, whereas dsRNAs against Drosha, Dcr-1 or Ago1 had no effect (Fig. S1C). Conversely, RNAi knockdown of Drosha or Ago1 led to a marked decrease in miRNA-mediated gene silencing while treatment with dsRNAs against LacZ, Dcr-2 or Ago2 had no effect (Fig. S1D).
We screened a collection of ~21,000 dsRNAs for those that impacted the siRNA and miRNA pathways. To assess reproducibility, dsRNAs targeting each positive emerging from the two primary screens were re-synthesized and tested multiple times using both assay systems. To minimize potential off-target effects, we also generated additional independent dsRNAs targeting each gene and assessed their impacts on the siRNA and miRNA pathways. Only genes represented by two or more independent consistently scoring dsRNAs were selected as final candidates. We found that Dcr-2 and Ago2 were among the siRNA pathway genes, whereas Drosha and Ago1 were among the miRNA pathway candidates (Fig. 1C and Table S1), providing an internal validation of each screen.
Recently, an extensive collection of endogenous siRNAs has been characterized in Drosophila (Czech et al., 2008; Ghildiyal et al., 2008; Kawamura et al., 2008; Okamura et al., 2008). The biogenesis and function of these endo-siRNAs depend upon canonical RNAi pathway components, including Dcr-2 and Ago2. In some cases, endo-siRNA production depends much more heavily on Loquacious rather than on the canonical Dcr-2 partner, R2D2 (Czech et al., 2008; Okamura et al., 2008). We tested all candidates emerging from the miRNA and siRNA pathway screens for their impacts on a sensor for an abundant endo-siRNA, esi-2.1 (Fig. S1E). In summary, 177 candidates were identified that affected at least one of the three pathways when knocked down (Fig. 1A). Among the 116 candidates for the siRNA pathway, 84 also altered the endo-siRNA pathway, and 70 the miRNA pathway. Significant overlap (69) was also observed between the endo-siRNA (132) and miRNA pathway (98) candidates. Notably, 54 candidates affected all three pathways.
Based on their annotations, candidates that affect small RNA pathways could be assigned to several functional categories. Notably, genes encoding RNA-binding/processing factors and translation factors were enriched by ~10-fold (Fig. 1B), since only ~2% of all Drosophila genes belong to this class (Lasko, 2000). These included splicing factors, RNA helicases, and proteins involved in poly-adenylation. Splicing factors could have scored in the siRNA pathway screen because the artificial hairpin transcript, which triggers silencing, carries an intron. However, neither the miRNA expression construct nor the endo-siRNA triggers contain intronic sequences, yet most splicing factors still impacted their function. Moreover, in a screen for RNAi pathway components in C. elegans, a number of splicing factors as well as the ortholog of the U1-associated factor, PSI, were identified (Kim et al., 2005).
Many candidates emerging from the screens showed direct protein-protein interactions, fit into functional modules or joined known multi-protein complexes (Tables S1 and S2). For example, six U2 snRNP proteins displayed a similar scoring pattern in the screen. We also observed common behavior for two U1 snRNP proteins, snRNP70K and CG5454, and for PSI, which physically and functionally interacts with snRNP70K (Labourier et al., 2001; Salz et al., 2004).
Knockdown of several ribosomal proteins impacted small RNA pathways. While this effect could be indirect, some ribosomal proteins have been implicated in the siRNA pathway. For example, RpL11 and RpL5 have been shown to reside in a protein complex containing FMR, Dmp68 and Ago2 in S2 cells (Ishizuka et al., 2002).
Silencing a number of proteasomal components consistently impacted all three small RNA pathways. Indeed, a regulatory subunit, Pros45, has previously been implicated in RNAi (Ulvila et al., 2006). We find that knockdown of Tbp-1, CG12000, or l(2)05070 led to a decrease in miRNA-mediated silencing and a concomitant reduction in miRNA levels (Fig. 2A). It is not yet clear whether these effects are direct or indirect.
Candidates affecting the miRNA pathway could impact miRNA biogenesis/stability or miRNA effector functions. To map candidates along the pathway, we examined the effects of their knockdown on steady state levels of the CXCR4 miRNA mimetic, on endogenous miR-2b, and on those of pri-CXCR4. Knockdown of several candidates led to consistent alterations in mature miRNA levels that paralleled effects on miRNA-mediated silencing (Fig. 2A and Table S3). For example, Drosha and Ago1 silencing reduced both miRNA levels and miRNA function, and Drosha silencing also led to a coincidental accumulation of pri-CXCR4. This class of positives likely affects miRNA processing and/or stability. Unexpectedly, silencing of Ago1 also had an impact on pri-miRNA levels. While this could represent a feedback mechanism, it is also possible that a miRNA indirectly regulates the metallothionein promoter used to express the pri-CXCR4 in this study. Another set of candidates (such as CG5514, CG3814 and CG2807) impacted miRNA-mediated repression without corresponding effects on mature miRNA levels. These positives most likely impact directly or indirectly effector steps within the pathway.
We also examined steady levels of esi-2.1 upon knockdown of candidates. As shown in Fig. 2B, knockdown of a group of candidates (e.g., CG7185 and snRNP70K) caused a decrease in esi-2.1-mediated gene silencing, which correlates with a reduction in esi-2.1 levels. This suggests involvement in siRNA production and/or stability. In contrast, knockdown of ubi-p63E, CG11700, or CG12000 caused a change in esi-2.1 activity that did not correlate with alterations in esi-2.1 RNA levels. Considered together, our analyses of the steady-state levels of small RNAs and their precursors following candidate knockdown provide clues on the placement of candidates within small RNA pathways.
bel emerged from our screen and from other studies as an RNAi pathway candidate (Kim et al., 2005; Ulvila et al., 2006). It encodes a DEAD-box RNA helicase, which is required for viability and in the germ line (Johnstone et al., 2005). We chose to validate bel both in animals and through biochemical approaches.
In order to assess RNAi efficiency in flies, we used transgenics carrying an inverted repeat of the white (w) gene under the control of the eye-specific GMR promoter (GMR-wIR; Fig. 3). These flies display a pale eye color due to strong suppression of white. Because bel is essential, we used an eye-specific mitotic recombination system to generate mosaics in which we could assay the impact of a bel allele (bel6) on w silencing in clones (Stowers and Schwarz, 1999). As previously described, eye cells homozygous for a Dcr-2 mutation, Dcr-2fsL811X, display a dark red color indicating loss of silencing (Fig. 3D and Lee et al., 2004). Eyes predominantly homozygous for bel6 are rough and small, suggesting that bel is required for cell viability. Importantly, patches of cells with increased pigmentation are observed in homozygous bel6 clones (Fig. 3G, arrow heads). We examined hsc70-4 mutant eyes, which are also small and rough, and found pigmentation to be unaffected, suggesting that the bel6 phenotype is specific (Fig. 3H). These observations suggest that bel6 mutant clones are defective in RNAi.
Next we tested whether Bel associates with RNAi components. Cytoplasmic extract was prepared from S2 cells and fractionated by gel-filtration. Individual fractions were immunoblotted using antibodies against Bel and components of RISC, including VIG, FMR, Ago2 or Ago1. The majority of Bel co-fractionates with Ago1, whereas a smaller fraction co-elutes with Ago2, FMR and VIG, components of the siRISC (Fig. 4A). We also immunoprecipitated FLAG-tagged Bel from S2 cells and found robust co-precipitation of Ago2, FMR or VIG (Fig. 4B). RNase treatment reduced interaction with Ago2 and VIG but not as substantially with FMR (Fig. 4B).
To test interactions of Bel with small RNAs, we expressed FLAG-tagged Bel or known components of the RISC together with an artificial siRNA (CXCR4) generated from a perfectly complementary hairpin expression construct. As expected, robust CXCR4 signals could be detected in the Ago2 or VIG complexes (Fig. 4C). Importantly, CXCR4 was also present in the Bel immunoprecipitate, as was esi-2.1, though they are probably bound directly to another protein in the complex. We conclude that Bel likely acts directly as part of the RNAi machinery, as it resides in a complex that also contains both protein and RNA components of RISC. Interestingly, more CXCR4 siRNA was present in the Ago1 immunoprecipitate than was detected in the Ago2 sample (Fig. 4C). While this could be attributed to some intrinsic characteristics of the CXCR4 siRNA mimetic, it is also possible that the coupling between miRNA processing and loading steps accounts for this observation.
We found that RpL22, a candidate from the screen, could co-immunoprecipitate components of the siRISC, including VIG, FMR, and Ago2, suggesting either a direct or indirect interaction. Similar behavior was observed for several other ribosomal proteins (Fig. 4B). These interactions seemed to display different degrees of dependence on RNA. For example, RNase treatment caused a moderate decrease in the levels of Ago2 in the RpL22 immunoprecipitate, whereas the levels of FMR and VIG remained unaffected (Fig. 4B). Similar observations were also made for the RpS7 and RpL21 samples. Moreover, both CXCR4 and esi-2.1 are present in the immunoprecipitates of a number of ribosomal proteins, including RpL22, RpS7, RpL21 and Rp49 (Fig. 4C). These observations indicate that these ribosomal proteins reside in large RNA-protein complex(es) that also contain core components of the RNAi machinery.
In Drosophila, siRNA and miRNA pathways have been viewed as being biochemically compartmentalized. However, the boundary between these pathways has been blurred by recent observations that, depending on the configuration of their precursors, miRNAs (and possibly siRNAs) can be partitioned between Ago1 and Ago2 (Forstemann et al., 2007; Tomari et al., 2007). Moreover, Loquacious plays roles both in miRNA biogenesis and in the production of some endo-siRNAs (Czech et al., 2008; Okamura et al., 2008). Intimate connections between siRNA and miRNA pathways are also suggested by the observation that knockdown of Ago2 leads to more pronounced silencing by miRNAs (Fig. 1C), possibly by increasing the access of Ago1 to miRNAs or other limiting components that were previously bound by Ago2.
Analysis of miRNA and siRNA screens shows extensive overlap between genes that impact these pathways. Suppression of many candidates reduces the efficiency of the target small RNA pathways, indicating that those genes might be components of siRNA- or miRNA-mediated responses. Silencing of a roughly equal number of genes increases silencing, indicating that they encode negative regulators of small RNA pathways. These so-called "enhancer of RNAi" phenotypes might indicate attractive targets for genetic manipulation or small molecule inhibitors that could increase the activity of RNAi in either experimental or therapeutic settings. It is worth noting that our analysis of steady-state levels of small RNAs upon candidate knockdown revealed that for some, enhanced silencing is correlated with increased levels of the small RNA silencing trigger, as is the case for the miRNA pathway candidates such as CG32721, mule, TH1 or flfl (Fig. 2A). In contrast, while knocking down CG2807 led to markedly enhanced silencing by the miRNA mimetic CXCR4, the steady-state levels of both CXCR4 and miR-2b significantly decreased. While these effects on the small RNA pathways could be indirect, these observations suggest that some of these negative regulators of RNAi are primarily involved in the biogenesis and/or stability of the small RNA silencing trigger, while others are implicated in the downstream effector steps.
Each pathway was uniquely or differentially affected by a number of genes (Fig. 1C). For example, knockdown of one class of genes (caf1, CG17293 and genes encoding ribosomal proteins L5, L21, L22 and S15) led to decreased silencing by exogenous siRNAs, but enhanced silencing by endo-siRNAs. Suppression of such a class of genes might enhance the production or loading of endo-siRNAs into RISC, thereby depleting the pool available for products of exogenously introduced dsRNAs, a model that has been previously proposed for some loci in C. elegans (Duchaine et al., 2006). Knockdown of another group of genes (hsc70-4, CG3825 and CG2577) decreased silencing by miRNAs but enhanced silencing by endo-siRNAs. A number of possibilities, including effects on small RNA sorting might account for these observations.
We validated Bel as a bona fide component of the RNAi pathway. Bel most likely functions at step(s) downstream of siRNA processing and loading, as neither steady state levels of esi-2.1 nor the levels of Ago2-bound esi-2.1 are affected by Bel knockdown (Figs. S2 and S3). Interestingly, Ago1 and Bantam are also present in the Bel immunoprecipitate, consistent with the co-fractionation of Bel with miRISC (Figs. 4B, C). Thus, Bel may also participate in the miRNA pathway. While none of the bel dsRNAs met the scoring criteria in the miRNA assay, they did trend consistently (Table S1).
Ago1 and Bantam were present in a number of ribosomal protein immunoprecipitates, and the association between Ago1 and these ribosomal proteins was abolished by RNase treatment (Figs. 4B, C). These observations are consistent with the notion that miRISC associates with the translation machinery. Both protein and RNA components of the siRISC are also present in these immunoprecipitates and that knockdown of a number of ribosomal proteins consistently leads to enhanced silencing by endo-siRNAs (Table S1). Thus, the integrity and function of the translational machinery as a whole may be impacting the small RNA pathways.
In summary, our comparative genome-wide screens (Table S5) generate a rich resource for further study of the three Argonaute-dependent small RNA regulatory pathways in Drosophila. These studies not only point to extensive overlap and interplay among small RNA directed silencing machineries in flies but also highlight specific players in each of the three pathways.
Detailed description of DNA constructs can be found in Supplementary Methods. S2-NP cells were maintained in Schneider's medium (Invitrogen) supplemented with 10% FBS and 1% pen-strep (Invitrogen). To generate the RZ-14 stable cell line, S2-NP cells were transfected with pRmHa-3-Firefly-long, pRmHa-3-Renilla and pRmHa-3-Renilla-hairpin together with pHS-neo, using Effectene (Qiagen). Transfected cells were selected and maintained in growth medium supplemented with 400 μg/mL G418.
Detailed description of the RNAi screening and bioinformatic analysis can be found in Supplementary Methods.
S2-NP cells were transiently transfected with DNA constructs for the miRNA assay (pMT-Renilla-CXCR4-6B, pMT-D05 and pRmHa-3-Firefly-long). Two days after transfection, ~3X106 cells were incubated in 1.5 mL serum-free Schneider's medium containing 10 μg of candidate dsRNAs in 6-well plates, and 3 mL serum-containing medium was added 45 minutes later. After 3 days of dsRNA treatment, cells were induced with 200 mM CuSO4 for 24 hours. A small aliquot of cells were subjected to luciferase assays to examine the effect of dsRNA treatment on reporter activity. Total RNAs were extracted from the rest of the samples using Trizol (Invitrogen). Northern blotting was performed as previously described (Czech et al., 2008). miRNA signals were normalized against that of the U6 RNA. A score was assigned to each sample based on the average results from two or four independent experiments. To quantify steady-state levels of pri-and mature miRNAs, semi-quantitative RT-PCR (q-PCR) assays were performed (Applied Biosystems) using primers specific for pri-Bantam, CXCR4 and U6.
Cells were transfected with expression constructs for epitope-tagged proteins, induced with 500 μM CuSO4 2 days after transfection, and harvested another 24 hours later. Immunoprecipitation was performed as described (Czech et al., 2008). For RNase treatment, the immunoprecipitates were split into two sets, and one was incubated in lysis buffer with 100 μg/mL RNase A at 4° C for 15 minutes prior to washing. For RNA immunoprecipitation, cells were transfected with protein expression constructs together with pMT-F12, an expression vector for CXCR4 derived from a perfectly base-paired precursor, and induced with 500 μM CuSO4 2 days after transfection. Cells were harvested and cell lysates prepared 24 hours later. About 80% of the cell lysates were subject to immunoprecipitation using M2 agarose beads. After washing, 10% of the immunoprecipitates were analyzed by Western blotting to verify the expression of the epitope-tagged proteins. Total RNAs were extracted from the remaining immunoprecipitates and cell lysates and analyzed by Northern blotting.
S2-NP cells were lysed in hypotonic buffer [20 mM Hepes (pH 7.0), 2 mM MgCl2, 0.2 mM CaCl2, 1 mM DTT], and spun at 30,000g for 20 minutes. The supernatant was spun at 200,000g for 2.5 hours, and the resulting pellet was subsequently resuspended in buffer A [20 mM Hepes (pH 7.0), 2 mM MgCl2, 1 mM DTT, 0.5% octyl glucoside, 400 mM KCl], and spun at 200,000g for another 2.5 hours. The supernatant was fractionated on Superose-6 HR10/10 (Pharmacia).
bel mutant flies (bel6) were from the Bloomington stock center and from Dr. Paul Lasko; EGUF flies were from the Bloomington stock center and from Drs. Thomas Schwarz and Stephen Stowers; and the GMR-wIR and dcr-2L811fsX flies were from Drs. Richard Carthew and Sara Cherry.
We thank members of the Hannon and Perrimon labs, especially Sara Cherry for helpful discussion, and Julius Brennecke, Ben Czech and Colin Malone for sharing unpublished data and reagents. We also thank the DRSC staff for technical support, the Bloomington stock center, Drs. Paul Lasko, Thomas Schwarz, Stephen Stowers, Richard Carthew and Sara Cherry for fly stocks. We are grateful to Dr. Phillip Sharp for the CXCR4 constructs, and to Dr. Paul Lasko for the Bel antibody. We are indebted to Drs. Bernard Mathey-Prevot and Richard Binari for critically reading the manuscript. R.Z. is supported by a Special Fellowship from the Leukemia and Lymphoma Society. This work was supported by grants from the NIH (N.P. and G.J.H.), and by a kind gift from K. W. Davis (G.J.H.). N.P. and G.J.H. are investigators of the Howard Hughes Medical Institute.
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Laila Shereen Sakr is a digital media theorist and artist working in computational art, live cinema, data visualization, and media activism. She is Assistant Professor of Film and Media Studies at UC Santa Barbara. Her work uses media analytics, visualization, and immersive storytelling techniques to map how participation in virtual worlds and networked publics have influenced the formation of a virtual body politic. This research led her to design the R-Shief media system for archiving and analyzing content from social networking sites, and the cyborg representation of VJ Um Amel. Shereen Sakr has shown in solo and group exhibitions and performances at galleries and museums across the Americas, Europe, and the Middle East, and has published extensively. She holds an M.F.A. in Digital Arts and New Media from University of California, Santa Cruz, an M.A. in Arab Studies from Georgetown University, and a PhD in Media Arts + Practice from the University of Southern California. Recent reviews appear in The Wall Street Journal, Science, The Chronicle of Higher Education, Fast Company, The Huffington Post, The Guardian, Voice of America, The Monocle, Art Territories, Digital Media and Learning, Egypt Independent, Mada Masr, Jadaliyya, and The Creators Project.
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Incoming senator Derryn "Human Headline" Hinch lambasts the anti-Islam policies of his new Upper House colleague, Pauline Hanson, mocking her call for CCTV cameras in mosques.
Topics: government-and-politics, elections, federal-elections, federal-government, religion-and-beliefs, royal-commissions, australia
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A Canberra man is sentenced to six months' jail for lying during the trade union royal commission hearings last year.
Topics: royal-commissions, law-crime-and-justice, canberra-2600, act
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Defence Force Vice Chief Raymond Griggs delivers a passionate speech to abuse victims and their families, praising them for their courage in coming forward and telling them their stories are "changing the ADF".
Topics: royal-commissions, law-crime-and-justice, sydney-2000, nsw
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A retired Australian Air Force commodore tells a royal commission there needs to be improvement in how sexual abuse investigations are carried out within the service.
Topics: royal-commissions, law-crime-and-justice, child-abuse, community-and-society, sydney-2000, nsw, australia


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Even the Sun Will Die: An Interview with Eckhart Tolle Audio Cassette – Audiobook, Unabridged
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Book Description
From insights into the way out of suffering to evidence that a new consciousness is rising, "Even the Sun Will Die" reveals that even following the tragedy of September 11, 2001, a miracle happens when we say "yes" to living in this moment and no other.
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To those who say it is "hard to follow" I would respond by saying that the fault does not lie in the CD, but elsewhere. To those who say they are put off by the author's voice or speaking mannerisims, I would respond that they should re-read his original work and quit focusing on the shallow forms we humans take and concentrate on the words! Have we come to this - that only good looking/perfect/great sounding people can sell anything? And to those who say the author is only repeating what he said in his origianl book, I repsond, of course! His original book had it all. He has been breaking it down into smaller and smaller pieces at the request of the public, and he has told us this in his previous interviews etc. He has already said that everything we need is in Power of Now, but because we want it in smaller pieces, he is giving it to us - only to get complaints. And for the people who complain that he did not address the 9-11 issue - read the label and listen to the introduction. This issue was addressed in those locations as well as in the message of the text - pay attention!
I listened to the audio CDs in my car and experienced a sudden shift, a deep understanding, accompanied by lightness and a feeling of expansion, although I had already read the book "The Power of Now". Make no mistake: this is a highly evolved being speaking to you. I understand that it is difficult for a fast-paced culture (like the American) to listen to the silence and laughter and hear the messages in it. This is the point. As to the 9/11 events: how appropriately he addressed this drama. Yes, drama. As it is merely drama that unfolds, but this IS NOT us. We are eternal being caught up in material reality in this incredible drama. Tolle knows how to bring people back to who they REALLY are.
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I've listened dozens of times and each time learn something in a deeper way. The fundamental teaching is of course the same as The Power of Now, yet without as much intellectual... Read morePublished on Dec 19 2003
As a fan of "The Power of Now" I eagerly awaited the arrival of an audio of more wisdom from Eckhart Tolle. "Even the Sun Will Die" was disappointing. Read morePublished on Aug. 8 2003
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Bid placed 5/25/2010 12:59 PM CST
Expired on 6/4
Bid placed 6/20/2010 9:55 PM CST
Expired on 6/27
Bid placed 7/2/2010 10:32 AM CST
Declined on 7/2
Do you have a hydralic trailor or can you do a wet launch?What is your height restriction?
no hydralic trailer . I need permits in each State for shipment possible wet lauch if concrete slab to be verified at which Marina possible ?? lift is allot easier
Bid placed 5/25/2010 2:59 PM CST
Declined on 6/19
Will you do a wet launch in fresh water in Austin? will there be any extra charges
Can't launch,Lifts required on both ends.
Bid placed 6/19/2010 10:52 AM CST
Declined on 6/29
Bid placed 5/14/2010 1:27 PM CST
Declined on 6/15
Bid placed 7/1/2010 3:39 PM CST
Bid placed 6/29/2010 3:11 PM CST
Expires 7/9 3:10 PM
Do you have references re: sailboat hauling clients.If so,I would like them. Thanks
With a hydralic trailer can you load and unload without a lift?Austin has a no. of ramps to do wet offload.Can you do wet launch?
Hi. Sorry for the delay in responding, was unloading a Catalina 30 at Lake Conroe. As for wet launching the only problem with a boat of that size is finding a steep ramp. Tried wet launching a 35' in Dallas last month and could not find a steep enough ramp. Owner had to end up calling a crane co. I will be glad to provide references but i am not allowed to send contact information thru uship until a job is booked. If you choose to book and are not satisfied with the references, your deposit can be refunded thru uship (I do not hold the deposit). Please check my recent feedback here on uship. Thanks, Steve
There are at least 3 ramps @ Lake Travis That are steep.Do you have any local knowledge of these ramps:-Mansfield Dam Park,Sail and Ski Marina(was Yacht Hbr.) and Emerald Point Marina??There maybe others and I can look.
I am not familiar with the Austin facilities but i do know a professional rigger that i can call for guidance if needed.
Steve,Thanks for your prompt replies.It is likely Paul Schmidt.If so I can do the call and save you time.He is at Sail and Ski Marina and I have talked about rigging.They have a crane on site to lift the mast.Let me know.
Actually this guys name is Keith. He works out of Kemah but travels and has rigged over 1000 boats. He is impressive. I know he works in Austin too. Regardless we can make it work. I am going have a temporary tongue extension for my trailer fabricated so it is easier to launch the big boats
yeah,ask him.He will have an unbiased opinion.
When can you transport the boat?
Is there an extra charge for wet launching?how much?
Explain a hydralic trailor and its extra versatility?
Can you let me know your target dates and let me see if I can work around them please? I have some flexibility in my schedule. No extra charge for wet launching. The only thing here would be like in Dallas, if there is no way to safely do it, you would have to take care of the crane, etc. The hydraulic trailer allows me to self-load a boat if it is on stands without the aid of a crane or marine lift. I can also self-load one which is sitting in a cradle. Not that this is of any value to you, it is just a specialized trailer and is very heavily constructed (weighs over 8,000 lbs.). Thanks again, Steve
They are doing the bottom job now and I expect it to be complete by weekend.So, as early after completing the bottom as you can-early next week?I will call T.M. so you can have a firm date.I am ready. Dave
Dave, you might want to give the bottom a few days to dry. The boat I dropped off yesterday had just had a bottom job and the vibration from the move at the support pads caused some rubbing of the nice new paint. It was just cosmetic, but please be aware that this can happen. All wide loads are shut down over the extended weekend so the middle or end of next week would be the soonest I could get to the boat (my trailer empty is oversize). Let me look more at my schedule and I will get back to you asap. Steve
Steve,I have revised my pickup date.There is a festival here on lake Travis on July,10thand so that week is too busy.The best time for pickup is July 12th and hopefully arriving here in Austin before thurs.As you are likely aware these facilities do not work on or near weekends and so even thurs. is likely to cause a delay in offloading.Is this possible in your schedule?Also what is your Max. height.Please reply ASAP as I need to make a decisions. Dave
Dave, The 12th would be fine. Hopefully I can load first thing in the morning. I need to be unloaded by late Wednesday if possible as my oldest son has been out of the country for a month and returns on Thursday. The max height without permits is 13'6" and my keel support is about 12" above the road. I have had no problem with height on boats up to 35' in length. Thanks, Steve
Bid placed 6/7/2010 8:54 AM CST
Canceled on 6/9
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text:
I am working in an application where we need to save objects in XML format, and load them later once required. For this I have used JAXB to marshall and unmarshall XMLs back to Java classes.
My problem is that the I have to change Java models sometime (by adding, renaming or deleting attributes), as a result, I will have incompatible saved XMLs which can't be bound back to the new class form.
To solve this, every time I have to do a change I take a copy of all the classes under a new package (named after its version) and apply the requested changes. And when saving an XML I save its version so that I can decide which package should be scanned by JAXB to unmarshall this XML.
My question is, Is there any other way to implement backward and forward compatibility using JAXB? If not is there any other technology that can support this?


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Midsomer Murders
Those Midsomer murderers are at it again! Series thirteen of ITV's tongue-in-cheek country classic catches yet more victims napping in sleepy villages whose Old English beauty looks too good to be true.
Explore Midsomer Murders country by car using the trail download. The trail features several attractions and there are some excellent pubs and towns on route, perfect for a day out or weekend away.
Download the Midsomer Murders Trail
View the Midsomer Murders Google Map
Download Midsomer Murders Episode Guide
Read how Midsomer Murders is popular not just in the UK!
A must for all Midsomer Murders enthusiasts...
Midsomer Murders On Location by Sabine Schreiner & Joan Street
Edited by Antony Richards. (£4.99 + P&P)
'The majority of the pretty villages that make up Midsomer country are actually strewn over four counties - Buckinghamshire, Berkshire, Hertfordshire and Oxfordshire. Here for the first time is a comprehensive guide to the gorgeous locations used in the filming the series.'
Exploring Midsomer The towns and villages at the murderous heart of England by Chris Behan (£12.99)
Available from Aylesbury Tourist Information Centre on 01296 330559 or Marlow Information Centre on 01628 483597.
Featured Attractions
Location: Milton Keynes, Buckinghamshire
Experience the Country is an outdoor/indoor pursuit centre set on a beautiful 800 acre country estate
The Roald Dahl Childrens Gallery and Bucks County Museum
Location: Aylesbury
Bucks County Museum in the heart of historic Aylesbury is a fascinating
place to visit.
Experience Roald Dahl Country
Location: Buckinghamshire
The Roald Dahl Trail reveals the secret of where to go and what to see for an absolutely squiffing day out.
Go Ape at Black Park and Wendover Woods
Location: Buckinghamshire
Why not get up in the trees in Buckinghamshire?
Midsomer Murder Mystery Tours
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Let us take you on one of our tours to Midsomer County. Choose your favourite episodes and see...
Hidden and Haunted Sites in Buckinghamshire
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Visit the sites where ghosts are said to roam and explore the hidden...


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Click here to try it for free
All subscribers will gain access to an archive of 60 issues:
Available to read:
- Online
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Available to read:
- Online
- iPad
- iPhone
- Android
Latest issue:
Is Hinkley Point 'C' worthwhile? Plus Brompton Wicker baskets, travelling in Japan, Mechanical Doping in cycle sport, Nano-Brompton pedelec conversion
A to B magazine is a quarterly publication which carries features and news on folding bikes, electric bikes, trailers, trikes, trains and alternative transport.
The trial issue offers a taste of Ear to the Ground industry news and gossip from 'The Mole' ; a comparative review of two new micro-folder bikes, the Sinclair A-bike and the Mobiky Genius; and the regular Workshop feature tackles a noisy Sturmey Archer gearbox.
If you have an iPad or iPhone you can read the latest issue as well as the extensive archive via the A to B app.
Android owners can also read the magazine with the Exactly app. Click here to download or search for Exactly on the Google Play Store.
For full access, just log in with your Exact Editions username and password when prompted by the app.
All subscribers will gain access to all 60 issues:
Copyright © 2016 Exact Editions Ltd.


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The Fascinating Life of Bonnie Raitt
Matt Mindlin
During a career that's - so far - spanned well over 40 years, Bonnie Raitt established herself as an indomitable force in the music community. Known for her brilliant guitar work, bluesy ballads, searing, soulful vocals and, of course, mass of flaming red hair, it turns out, there's plenty about Raitt most don't know.
Her backstory provides a fascinating trajectory in terms of her artistry, accolades, and the sequence of events that brought her to icon status. Bonnie may play the blues, but her career is multi-hued. Here then are a few facts that casual fans may not know about this powerful songstress.
5. On Broadway
Bonnie is the daughter of Broadway legend John Raitt, an actor capable of projecting both an indelible image of dominant, confident masculinity and a riveting voice ready made for the musical stage. The elder Raitt made his mark in such legendary shows as Carousel, Oklahoma, and the Pajama Game, roles that forever identified him with the golden age of the Broadway musical. Her mother, the widely heralded singer and pianist Marge Goddard, was no slouch either in terms of musical DNA.
Upcoming Events
Grand Finale: 2016 Piano Academy Winners
TicketsSun., Jul. 24, 5:00pm
Alien Ant Farm
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Gwen Stefani: This Is What the Truth Feels Like Tour
TicketsWed., Jul. 27, 7:00pm
Premier Parking: Gwen Stefani July 27, 2016
TicketsWed., Jul. 27, 7:01pm
4. Si Se Puede
In spite of her upbringing, music wasn't Raitt's first career choice. She majored in Social Relations and African Studies at Radcliffe. And while folk and blues attracted her interest early on - she got her first guitar at age 8 - she was equally intrigued by politics and the changes in the social strata during the tumult of the 1960s. Raitt's commitment to activism never faltered; she's used her fame to support any number of important causes, from fighting apartheid and the spread of nuclear proliferation, to preservation of the environment and working for the rights of the oppressed.
3. White Girl Sings the Blues
Coming from a well-to-do show business family, Raitt seemed an unlikely candidate to pursue the blues. And yet, under the tutelage of such legends as John Lee Hooker, Muddy Waters, Fred McDowell, Sippie Wallace, and Son House, she quickly established credibility. Prior to Raitt, most better known white blues musicians - especially those who fronted bands with guitars in their hands - were male and British, like Eric Clapton, Jeff Beck, and Jimmy Page. Yet Raitt established a new feminist template, one that was later emulated by the likes of Rory Block, Susan Tedeschi, and Lucinda Williams.
2. Later in the Game
Raitt didn't score her first big hit until half a dozen years into her career when her remake of "Runaway" sprinted to number one. Until that time, Raitt's albums were praised by the critics, but mostly ignored by the record-buying public. "Runaway" prompted a bidding war between her then current label, Warner Bros., and Columbia Records. Raitt opted to stay with Warner, only to have the label dump her along with several other established artists on the roster little more than six years later.
1. Can You Hold This Grammy? I Have to Grab My Keys.
When it comes to Grammy nods, Raitt is one of the most successful female artists ever. She's chalked up ten of them over the course of her career. Her defining moment came when Nick of Time, her debut album for Capitol Records, helped garner four Grammys and took her to the top of the charts. Both follow up albums, Luck of the Draw and Longing in Their Hearts, packed her mantle with plenty more of these gold-plated gramophones. It took 20 years, but Bonnie was finally a credible commercial success. And she hasn't stopped since.
Bonnie Raitt with Paul Brady. 8 p.m., Saturday, November 30, at Hard Rock Live, 1 Seminole Way, Hollywood. Tickets cost between $59.50 and $89.50 plus fees. Call 954-797-5531, or visit hardrocklivehollywoodfl.com.
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Full-service digital marketing agency Geary Interactive has acquired Fathom Online, a search engine marketing firm. Though Geary already offered search marketing services, the company says it will expand it demographic reach as a result of the merger.
Geary CEO Andreas Roell said, "The combination of Geary and Fathom Online provides a compelling platform for today's digital marketing landscape. Together, we offer powerful capabilities and deep relationships with publishers and engines, expanded end-to-end customized marketing solutions, a national presence and extensive industry category experience."
The acquisition also highlights the need for integrating SEM campaigns with a larger online marketing strategy.
"Search engine tactics are the most popular element of a digital marketing campaign. In order to effectively generate search demand and convert customers' search clicks, the campaign must also include a strong integration with web development, paid and organic digital media planning and data analytics," said Roell.
Related reading
Google launches imported call conversions
If the internet killed the phone call, the smartphone has revived it.
Will Pokémon GO become a marketing platform for local businesses?
Nintendo's Pokémon Go, a free augmented reality (AR) mobile game, is on fire.
Five most interesting search marketing news stories of the week
Welcome to our weekly round-up of all the latest news and research from around the world of search marketing and beyond.
Eight key improvements from an ecommerce site relaunch
Ecommerce site Simply Hammocks relaunched a few months ago, and has so far achieved some impressive results.


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Awesomely Random fluffing HUMDINGERS!
here is a real humdinger for you ladies:
im sitting at the computer i look to my right and i can see the screen of BILs phone he is on craigslist personals and...there is a picture of a dudes penis on the screen, i glance back a few mins later and there is another penis on the screen...i swear to god he is gay but he is married and has a kid from a past relationship wow
Kaden Robert -Born April 7,2012
Sarah Jane. FTM to Jake 3.29.12.
Deep inside, every woman is a princess. And every princess has a dragon.
Sararie, if he's married than why is he looking at personal ads on there? And right in front of you...weird!
and she loved a little boy very, very much - even more than she loved herself. <3
idk this dude is weird im not sure he is even legally married they got a license but had a friend sign it and im not sure they even sent it in but anyways...wow his "wife" was telling me she has found gay porn on her computer and phone and TMI but she said he obsessed with anal lol
dh and i are both right here im like WTF CREEPER
Kaden Robert -Born April 7,2012
btw i would love a fork to my eyeballs at this moment haha. but that channing tatum picture is delightful
Kaden Robert -Born April 7,2012
Learn more
Look how far your child has come.
Review your child's growth milestones.
Read about 4 years 2 months : Week 2.
Your growing child
Your 4-year-old: Ready to read?
Your 4 1/4-year-old: Physical milestones
Your 4 1/4-year-old: Peer pressure
Your 4 1/4-year-old: Curiosity
Your 4 1/4-year-old: Learning to share
Learn more
What's next for your growing child?
Get a glimpse of what's to come.
Read about 4 years 4 months.


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Where in the world is Richard Stallman?
by Jeanne Rasata Contributions — Published on Mar 11, 2010 10:39 AM
President and founder of the Free Software Foundation Richard Stallman has an extensive travel schedule and frequently gives talks about free software all over the world.
If you would like Richard Stallman (RMS) to speak at your event, please contact firstname.lastname@example.com.
If you would like RMS to come to your school and speak, try asking professors in IT-related departments if they would like to bring him. They can contact firstname.lastname@example.com.
You can find video and audio recordings of many of Dr. Stallman's speeches and lectures at audio-video.gnu.org.
Please note: Stallman has never had a Facebook account. He has never posted on Twitter, but has a Twitter account for posting comments on other sites. (Learn more.)
|Start Date
|Jul 24, 2016 12:00 PM
|New York, New York
|Richard Stallman - "Freedom and Privacy in Our Lives, Our Governments and Our Schools" (The Eleventh HOPE, New York, NY)
|Aug 11, 2016 06:00 PM
|Montreal, Canada
|Richard Stallman to speak in Montreal, Canada
|Aug 20, 2016 06:15 PM
|Pittsburgh, PA
|Richard Stallman - "Free Software and Your Freedom" (Abstractions - Pittsburgh, PA)


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Band Striped Scarf
Product Actions
Felted cashmere scarf with band stripes and contrast fringed edges.
Style # 503047540
- Approximately 28" length x 11" width
- Available in Ivory/Grey/Charcoal
- Imported
- Cashmere
- Hand wash
Style # 503047540
This luxurious and philanthropic line was launched by a former human rights lawyer who sources hand-crafted treasures made around the world to use in designing a collection. The artful designs celebrate ancient manufacturing techniques and exceptional craftsmanship from around the globe.
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If you prefer email, we can be reached anytime at email@example.com.
Free Shipping & Returns on all U.S. Orders. See Details.


text:
IRVINE, CA-A rapid growth in rental income is a byproduct of fundamental shifts in the housing market, driven by a large increase in affordability of investment properties and rising rents, according to CoreLogic, a provider of residential property information, analytics and services here. The firm's December MarketPulse report shows that weak wage income and job growth has spurred rental demand, and residential investment is contributing to economic growth in line with post-recession history.
In the report, CoreLogic's chief economist Mark Fleming and principal economist Sam Khater report that residential investment, which has averaged historically only 5% of GDP, is now contributing 12% of the overall low BDP growth rate. And while it took a full two years to reach this point, residential investment is now providing the post-recession boost it normally does.
Also, CoreLogic's Aurora Bristor points out in the report that multifamily rents are still floating high, with the single-family rental market more of a draw to foreclosed homeowners who brought their volatile circumstances to market.
In some markets, the employment news is more encouraging than in the country as a whole. For example, as GlobeSt.com previously reported, strong employment gains and fierce competition for single-family homes in Phoenix has fueled demand for rental properties here that will persist well into the new year, according to a fourth-quarter apartment market report by Marcus & Millichap.
*Charts courtesy of CoreLogic.
For the full report, click here.


text:
Book your room at Days Inn Auburn
Get an overview of this hotelMotel with free breakfast, near Emerald Downs
Situated in Auburn, this motel is within 3 mi (5 km) of Emerald Downs, The Outlet Collection, and Muckleshoot Casino. Soos Creek Botanical Garden and Pacific Rim Bonsai Collection are also within 6 mi (10 km).
Property Features
This smoke-free motel features an outdoor pool, a 24-hour front desk, and RV/bus/truck parking. Free continental breakfast and free WiFi in public areas are also provided.
Room Amenities
All 68 rooms provide conveniences like microwaves and coffee makers, plus free WiFi and TVs with cable channels. Other amenities available to guests include free local calls, safes, and desks.Would you like to give us feedback? Share it! Opens in a new window
Days Inn Auburn1521 D Street NE Auburn WA 98002
Hotel Amenities
Hotel Amenities
Guests can enjoy a complimentary breakfast each morning. Public areas are equipped with complimentary high-speed wireless Internet access. This Auburn motel also offers an outdoor pool and RV/bus/truck parking. Complimentary self parking is available on site.
Days Inn Auburn is a smoke-free property.
- 24-hour front desk
- Free breakfast
- Free self parking
- Free WiFi
- Number of floors - 2
- Outdoor pool
- RV, bus, truck parking
- Smoke-free property
- Total number of rooms - 68
Family Friendly Amenities
- Free Wi-Fi
- Microwave
- Outdoor pool
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking, RV, bus, truck parking
Room Amenities
- Access via exterior corridors
- Air conditioning
- Cable TV service
- Coffee/tea maker
- Connecting/adjoining rooms available
- Cribs/infant beds available
- Desk
- Free local calls
- Free WiFi
- In-room safe
- Microwave
- Television
Where to Eat
Guests are offered a complimentary continental breakfast each morning.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
Hotel Policies
Check-in time starts at 2 PM
Check-out time is 11 AM
Payment types
- Pets allowed for an extra charge of USD 20 per pet, per night
- Dogs only
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Pet fee: USD 20 per pet, per night
Hotel Name
- Days Inn Auburn Federal Way
- Days Inn Federal Hotel Auburn Way
- Days Inn Auburn/ Federal Way Motel
- Days Inn Auburn/ Federal Way
- Days Inn Auburn Motel
We should mention
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.


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Been staying at Little America for the last 20 years. Recently stayed both going and returning from Mesa Verde. Rooms are always clean, large and comfortable. Enjoy walking the newly expanded hiking trail each morning. . Staff is always helpful and available. Restaurant is very convenient. My wife and I truly feel this is our home away from home....Flagstaff and environs is the top of the list as far as experiencing the Southwest.....
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- Also Known As:
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