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The precision with which X-ray crystallography and nuclear magnetic resonance (NMR) have provided structural models of biologically active and inactive conformations of countless proteins belies an easily overlooked dilemma. Proteins are inherently dynamic, exhibiting conformational freedom on timescales from picoseconds to seconds, implicating structural rearrangements that are essential for their biological function.1 Classical structural biology determines static models, that afford little insight into the underlying conformational equilibrium. The role that structural dynamics play in biological processes can only be understood by characterizing all thermally accessible protein conformations and their populations.2
NMR spectroscopy is uniquely sensitive to the presence of conformational dynamics in solution. Residual dipolar couplings (RDCs) measured in weakly aligned proteins,3, 4 scalar couplings, and chemical shifts,5–7 probe motions occurring on timescales faster than 100 s of microseconds. These parameters therefore offer general tools to characterize protein motion on physiologically important timescales.8–10
A common approach to the dynamic interpretation of RDCs is to combine experimental restraint terms with a classical potential-energy force field to develop a conformational ensemble in agreement with experimental data.11–14 RDCs have also been exploited to characterize the conformational space sampled by the protein backbone either by fitting experimental data to determine angular excursions of internuclear bond vectors,15–18 or in comparison with different levels of accelerated molecular dynamics (AMD)19, 20 to describe the most appropriate ensemble.21, 22 Comparison of motions modeled using the Gaussian axial fluctuation (GAF) model, with ensembles derived from restraint-free AMD, demonstrated that such methods can provide a convergent description of protein motion.18, 23
The general application of these approaches is hindered by the amount of experimental data that are required to accurately describe the motion. For many experimental systems it is not possible to measure RDCs in a sufficiently diverse combination of alignment media to allow the application of analytical approaches. Comparison with AMD, on the other hand, supposes that simulations carried out at the "optimal" level of acceleration correctly describe all accessible conformations and their populations. Existing protocols18, 21, 22 are therefore poorly adapted to the quantification of modulations in the Boltzmann ensemble as a function of environmental conditions or the presence or absence of a partners.
We present a general method that exploits experimental RDCs to map the free-energy landscape occupied by folded proteins in solution, determining populations of accessible conformational sub-states contributing to the dynamic equilibrium. The method initially exploits multi-level AMD simulation, flooding the conformational space available to the protein as completely as possible to sample different sub-states, which are combined to provide an extensive pool of conformers, comprising both high-and low-energy conformations. Boltzmann-weighted ensembles are then assembled by comparison with experimental NMR data. Ensemble selection is achieved using model-free interpretation of RDCs combined with a specifically designed genetic algorithm. The approach is termed SUPERNOVA (sub-state populations on potential-energy surfaces using restraints from NMR spectroscopy and conformational oversampling).
The accuracy of SUPERNOVA, and its robustness against bias in the pools of structures from which the ensembles are selected, were tested using a synthetic dataset simulated from a hypothetical system. Two 200 ns MD simulations of the SH3C domain from CD2 AP were performed (see the Supporting Information). The first, referred to as "A", sampled the native fold, and the second, "B", an alternative conformation, that was adapted in the loop region from residues 32 to 44 (Figure 1). 1000 structures were retained from each trajectory (target A and target B) from which RDCs were simulated, while 10 000 different structures from the same trajectories constituted sampling pools (pool A and pool B) for selection. Conformers from target A and target B were combined with populations PA=100, 90, 75, 50, 25, 10, 0 % and PB=(100−PA) %. Noise-modulated RDCs were simulated from these targets using four orthogonal alignment tensors (supporting information). Variation of RDCs and order parameters (S2NH) between targets are shown in Figure 1.
SUPERNOVA selections aimed at the seven targets described above were initiated from seven different pools, containing the varying proportions of pools A and B. The results are summarized in Figure 1, showing the proportion of selected structures having the A or B loop conformation as a function of the pool composition. For calculations performed with a full set of RDCs (1DNH, 1DCC, 2DCHN and 1DCN from all four media), the proportion of A-type structures selected matches the target to within ±1 % for all targets and pools. Additional tests show similar accuracy for populations as low as 5 % (Figure 1 D). Figure 2 illustrates typical selected ensembles, in the {ϕ/ψ} space, using principal component (PC) analysis. SUPERNOVA correctly and reproducibly identifies populations of major and minor species (90:10) in spite of unfavorable initial bias (10:90). Repetition using sparser data identifies populations as low as 10 % using only 1DNH from three media, or four RDCs from one medium. 1DNH from one medium is insufficient to accurately determine sub-state populations (Figure 2 E).
Application of SUPERNOVA to experimental data requires that the ensemble of conformers in the sampling pool adequately covers the conformational space available to the protein. To address this challenge, we initially identify the level of acceleration that best reproduces experimental data, using published AMD protocols.18, 22 Additional sampling is then achieved using higher levels of acceleration. Distinct conformational sub-states in the potential-energy landscape are identified from all AMD calculations, from which standard MD simulations are carried out, ensuring the energetic viability of all conformers in the pool, as well as allowing analysis of fast motional properties. Conformational sub-states that are populated in different proportions to those present at the optimum level of acceleration, are then combined as a function of the experimental data.
The selection protocol was applied to an extensive set of experimental RDCs measured from the protein SH3C from CD2AP dissolved in 10 alignment media.18 The optimal number of structures within the ensemble (40 in this case) was determined using systematic cross-validation, removing one entire dataset from each of 10 analyses (Figure 3 a). Figure 3 shows S2NH of selection and pool, compared to fast (<4 ns) motions determined from spin relaxation.24 Critically, the analysis was repeated using only 1DNH from three alignment media, giving identical S2NH profiles (Figure 3 E), confirming that the procedure is robust when fewer data are available. Statistical uncertainty in the ensemble description was assessed using noise-based Monte–Carlo (MC) simulations. This uncertainty is expressed in terms of error bars of S2NH or dispersion in PC or dihedral angle analysis (Figures 3 and 4).
The n-SRC loop, comprising residues 36 to 42, exhibits the most motion and shows clear modulation of the population of different sub-states relative to the pool ensemble for residues 37 and 40. Residues 18 and 19 also show population redistribution, resulting in a more flexible equilibrium than present in the pool (Figure 3). {ϕ/ψ} distributions of the three regions in which slow motions of significant amplitude are present again highlight population redistribution (Figure 4). PC analyses of pool and selections (the three highest populated modes account for 30 % of the total motional amplitude) maps the accessible potential-energy landscape (Figure 4). Populations of different sub-states can be estimated on this basis (see Table S2 and Figure S5 in the Supporting Information). Representative structures of the major conformation, and two weakly populated sub-states are shown in Figure 4. PC analysis also identifies the most significant component of collective motion, involving the same loops (19–20 and 37–43).
Figure 4 shows a representative SUPERNOVA ensemble of 40 structures, indicating the position of the three regions exhibiting large-amplitude slow motions. The conformational sampling occurring within three of the most populated sub-states is also shown, corresponding to motions sampled on timescales up to the nanosecond range. Interconversion between the sub-states evidently occurs on the slower timescales (nanosecond to microsecond scales) defined by RDCs and chemical shifts. This separation of timescales is an essential feature of AMD-based RDC analysis, allowing for the differentiation between motional averaging regimes that are specific to each NMR parameter.18, 21, 22
Reproduction of entire RDC datasets from different media not included in the selection improves significantly compared to those calculated from the pool (Figure 3). Similarly 15N, 1HN, 13Cα, and 13Cβ backbone chemical shifts calculated using the program SHIFTX25 and averaged over the ensembles are significantly better reproduced by the RDC-based selection than the pool. All chemical shifts from the loop regions exhibiting slower motions show improved reproduction compared to the pool and to the individual structure that best fits all RDCs (average improvements of 22 and 30 %, respectively; see the Supporting Information). SUPERNOVA was also tested against an alternative pool composition, derived from three different fully solvated MD simulations (a total of 1.5 μs). This pool samples conformational space differently for a number of amino acids, but converges to a very similar selection, in terms of {ϕ/ψ} distribution, PC analysis, and S2NH (Supporting Information).
The growing volume of direct experimental evidence indicating the importance of weakly populated states for the function and malfunction of proteins,26 highlights the importance of developing methods that can provide a quantitative molecular description of all sub-states present in solution. The SUPERNOVA approach addresses this central question by combining solutions to four principal difficulties that have hindered progress in the resolution of quantitative conformational sub-state populations.
Ensemble analysis of RDCs has suffered from the difficulty of predicting the physical alignment of the protein in all but the simplest cases of steric alignment.27 We bypass this problem, generating a common mathematical solution reporting on all alignments, but more importantly, providing a measure of data reproduction by the ensemble of conformers that is essential to the selection algorithm.28 Secondly, we present an approach that is demonstrably robust at manageable experimental costs, with as few as three sets of 1DNH or different peptide plane RDCs from a single medium, both achievable for a large number of proteins. Thirdly, a genetic algorithm combines members of a large ensemble of energetically viable conformers into sub-ensembles in agreement with the data,29 avoiding the unpredictable effects of combining experimental and physical terms in a hybrid force field. Finally, the structural pool is generated using an enhanced sampling approach, based on multi-level AMD simulation, in which conformational space is over-sampled following an initial comparison to experimental data. Conformers from all simulations are combined into a pool from which sub-ensembles are selected, allowing for modulation of the rugosity of the potential-energy landscape as a function of experimental data.
SUPERNOVA remains accurate in the presence of relatively sparse experimental data, allowing the application of cross-validation procedures that demonstrate its predictive power. Crucially, the limits of the resolution of each experimental dynamic description can be readily established a posteriori, using MC noise-based analyses as shown in the application to SH3C from CD2AP. In this case SUPERNOVA identifies the presence of motions in the nanosecond to millisecond range, in three surface loops, two of which are involved in recognition of physiological partners.24 Future comparison of slow dynamic interconversion between sub-states in the free form of the protein with those present in physiological complexes will provide fascinating insight into their role in molecular recognition.
In conclusion, the combination of RDCs with innovative approaches to the efficient sampling of conformational space and specifically designed ensemble selection, provides access to dynamic averaging occurring on timescales extending from the picosecond to the millisecond. RDCs characterize the presence of dynamics on an amino-acid-specific basis, whereas AMD and ensemble matching map these motions within the accessible conformational space. This combination is predictive of independent experimental data, and provides a significantly better description of protein dynamics than static or alternative dynamic descriptions. The method is shown to provide statistically meaningful ensemble descriptions of protein motions, and is viable using experimental NMR measurements that are accessible for a large population of soluble proteins, ensuring a broad applicability of the approach to the study of physiologically relevant protein dynamics.


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A 3-D carbon nanotube mesh enables rat spinal tissue sections to reconnect in culture.
Faces for Fighting?
June 10, 2014|
Scientists propose that hominin facial bones evolved for protection against the powerful blows of combat.
Daily News Roundup
June 10, 2014|
Scientists propose that hominin facial bones evolved for protection against the powerful blows of combat.
June 9, 2014|
An advisory committee for the BRAIN Initiative says that to fully fund the goals of the neuroscience research program, taxpayers should fork over $4.5 billion.
May 23, 2014|
The International Institute for Species Exploration announces its picks of novel species discovered in the past year, including a carnivorous mammal, a tiny shrimp, and a fungus.
May 23, 2014|
Sperm-sex–sensing sows; blocking a pain receptor extends lifespan in mice; stop codons can code for amino acids; exploring the tumor exome
May 21, 2014|
A watchdog group claims that the federal agency improperly influenced the investigation of a study involving the treatment of preterm infants.
May 15, 2014|
The funding agency will require grant applicants to address sex balance in all future cell and animal studies.
May 8, 2014|
Disgraced psychology researcher Marc Hauser, who was found guilty of data fabrication and falsification during his time at Harvard, publishes two new papers.
May 7, 2014|
In a proof-of-principle study, scientists show that the three-dimensional shape of a cancer cell genome can reliably classify subtypes of human leukemia.
May 1, 2014|
Researchers unearth little evidence to suggest modern humans are superior to their Neanderthal ancestors.
April 11, 2014|
Researchers collate a list of the 100 most rare and unique avian species facing extinction.

subset: commoncrawl


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Jason Bourne
Jason Bourne is a film that, as a fan of the series, I kept trying to like. It just wouldn't let me.
Jason Bourne is a film that, as a fan of the series, I kept trying to like. It just wouldn't let me.
A wrenching and ultimately inspirational film about college linebacker Steve Gleason's battle with ALS.
"The Ballad of Narayama" is a Japanese film of great beauty and elegant artifice, telling a story of startling cruelty. What a space it opens…
Patrice Leconte's "Monsieur Hire" is a tragedy about loneliness and erotomania, told about two solitary people who have nothing else in common. It involves a…
Ken Loach's "I, Daniel Blake" wins the Palme d'Or at the 69th Cannes Film Festival.
Barbara Scharres on the cats of Cannes 2016.
The complete list of movie recommendations from writers at RogerEbert.com.
Writers at RogerEbert.com pick their favorite cinematic remedies to elevate their moods.
Writers at RogerEbert.com pick their favorite movies featuring star-crossed lovers.
Writers at RogerEbert.com pick their favorite movies featuring aliens and UFOs.
A table of contents featuring our coverage from the 2016 San Diego Comic-Con.
A report from Comic-Con 2016 on the various ways to celebrate the 50th anniversary of "Star Trek."
"Due Date" is nearly a down-market retread of the great comedy "Planes, Trains and Automobiles." It pairs up Robert Downey Jr. and Zack Galifianakis in the Steve Martin and John Candy roles, puts them in a car together, sends them down the highway, and doesn't neglect to rip off not one but two car doors.
The first film by director Todd Phillips since his blockbuster "The Hangover" cheerfully includes some of the same raunchy humor and the same dogged persistence in the face of overwhelming character defects. It's not as funny, but few films could be, and it does have some very big laughs. Unfortunately, the story holding them together isn't as successful.
Peter Highman (Downey) and Ethan Tremblay (Galifianakis) have a Cute Meet, or more of a Brute Meet, at the Atlanta airport, when Ethan's beater knocks off the door of Peter's Town Car. Peter is a neat, precise architect with a schedule to meet. Ethan is a shaggy man-child who confesses that on the way to the airport he and his buddy split a six pack — of 40s.
Ethan is like Joe Btfsplk, that character in "L'il Abner" who always had a black rain cloud over his head, with the difference that the cloud is over the head of whoever he's standing next to. Ethan efficiently destroys Peter's trip by switching carry-ons with him, shoving his hairy belly into his face (under stress, to be sure), and getting them both thrown off the flight by insisting on using the words "bomb" and "terrorist." That scenario leads to a run-in with TSA agents (who, oddly enough, seem to be morphing into Hollywood's villains du jour).
All cross-country trips involving odd couples require deadlines. Peter agrees to accept a ride in Ethan's rental car only because he must be back in Los Angeles with his wife, Sarah (Michelle Monaghan), who will deliver their child very soon by C-section. Ethan needs to get to L.A. because he dreams of becoming an actor. If you doubt Ethan Tremblay has a future in acting, simply consider Zach Galifianakis.
There are stops along the way to visit Western Union, Peter's old buddy (Jamie Foxx), and Ethan's practitioner in "herbal medicines," a pot dealer played by the scene stealer Juliette Lewis. Those visits are funny. There are also running gags involving Ethan's beloved pet dog and a coffee can containing the ashes of his father. When Ethan performs the rather private act of pleasuring himself in the car's front seat, it's not funny, but when the dog is inspired to copy him, it is. No matter how much I think, I can't decide whether training a dog to do that is cruelty to an animal.
So the movie probably contains enough laughs to satisfy the weekend audience. Where it falls short is in the characters and relationships. "Due Date" may offer enough, but with the example of the classic "Planes, Trains and Automobiles," it could have offered more.
Downey and Steve Martin are more or less a stand-off. But John Candy (and director John Hughes) made his character earnest, inept and lovable. The Galifianakis character seems more an exercise in passive aggression. He lacks social skills so urgently it's hard to believe he doesn't know what he's up to. Surely no one could be that obnoxious by accident. In comedy it helps if we have a bottom line of affection for the characters, and Galifianakis and Phillips make affection awfully hard to feel for Ethan.
There's a moment in "Planes, Trains and Automobiles" when the Steve Martin character thinks he has seen the last of John Candy, and then pauses, reconsiders and returns. There's a moment something like that here, but frankly, we don't know why the Downey character returns.
This movie is trying to kill these women, but they endure.
"It's a madhouse!"
A Blu-ray review of "Batman v Superman: Dawn of Justice (Ultimate Edition)," arriving July 19.
Women are nicer than men. There are exceptions. Most people of both sexes are probably fairly nice, given the nat...


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WASHINGTON, DC—US banks stepped up their commercial real estate loan lending in the first quarter while easing terms, according to the April 2014 Senior Loan Officer Opinion Survey on Bank Lending Practices.
On balance, the Fed reported, banks eased their lending policies for commercial and industrial and commercial real estate loans. At the same time, these institutions reported stronger demand for both types of loans over the quarter.
More domestic banks reported that they had eased rather than tightened standards on each of the three categories of CRE loans included in the survey: construction and land development loans, loans secured by nonfarm nonresidential structures, and loans secured by multifamily residential structures.
In each of these categories, reports of stronger demand also outnumbered reports of weaker demand.
The April survey also included a special question regarding changes in specific lending policies for CRE loans over the past year.
During the past 12 months, many more domestic banks reported having reduced rather than increased spreads on CRE loans. More domestic banks also reported having eased rather than tightened policies regarding the maximum size and maturity of such loans and having increased rather than decreased the market areas served.
These banks indicated few changes in their policies for debt service coverage ratios, loan-to-value ratios, or requirements for take-out financing.
In addition, several foreign respondents reported having decreased spreads, while few reported changes in other terms.


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Is there a way to bypass or remove the file lock held by another thread without killing the thread?
I am using a third-party library in my app that is performing read-only operations on a file. I need a second thread read the file at the same time to extract out some extra data the third-party library is not exposing. Unfortunately, the third-party library opened the file using a Read/Write lock and hence I am getting the usual "The process cannot access the file ... because it is being used by another process" exception.
I would like to avoid pre-loading the entire file with my thread because the file is large and would cause unwanted delays in the loading of this file and excess memory usage. Copying the file is not practical due to the size of the files. During normal operation, two threads hitting the same file would not cause any significant IO contention/performance problems. I don't need perfect time-synchronization between the two threads, but they need to be reading the same data within a half second of eachother.
I cannot change the third-party library.
Are there any work-arounds to this problem?


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Accepting new patients
Accepting new patients
3010 Westchester Ave
Purchase, NY 10577 Phone number & directions
Purchase, NY 10577 Phone number & directions
License & Identifications
A medical license is required for a doctor to practice medicine in a particular state. Requirements vary by state but most require, at a minimum, post-graduate training in the doctor's specific specialty. An NPI number is a national identifier unique to an individual health care provider and is managed by the Center for Medicare & Medicaid Services (CMS).
Learn more about Dr. Pasquale Vascimini:
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The overall average patient rating of Dr. Pasquale Vascimini is Excellent.
Education & Awards
Dr. Vascimini graduated from New York University.
Home > Find a Dentist > NY > Purchase Dentists > Dr. Pasquale Vascimini, DDS
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- Additional Information
- Dr. Pasquale Patrick Vascimini DDS is a male dentist and practices in Family Dentistry.
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- Dr. Pasquale Vascimini has 1 office location.
Dr. Pasquale Vascimini practices Family Dentistry near Purchase, NY.
See Purchase Dentists.


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La Crosse Fish and Wildlife Conservation Office
Biologist Pilot Study Reveals Intersex Condition in Largemouth Bass from Lower Des Plaines River, Illinois
tissue of a largemouth bass collected from the Dresden Reach of the Illinois
River Waterway, Illinois, U.S.A. in 2014. Credit: Mark Fritts, USFWS
intersex analysis during 2014 in the Dresden
Reach of the upper Illinois River Waterway,
Illinois, U.S.A. Credit: Map courtesy of US
Geological Survey
In recent nationwide studies, one of the most commonly used biomarkers to assess the effects of EDCs on fishes is the presence of eggs in male fish testes (i.e., intersex condition). Previous studies have indicated that Chicago-area streams have relatively high concentrations of EDCs known to induce intersex condition. Many of these streams also feed eventually into the lower Des Plaines River and the Illinois River Waterway. Thus, during spring 2014, Illinois Natural History Survey Fish Biologist Mark Fritts, who is now a biologist with the La Crosse FWCO, headed an EDC pilot study in the Dresden Island reach of the Illinois Waterway near Joliet, Illinois. The objective of the study was to survey the severity of intersex in male largemouth bass in an area directly affected by surface runoff and wastewater effluents from the Chicago Metropolitan Area. Previous studies have shown that largemouth bass are known to be very vulnerable to EDC exposure. Largemouth bass were collected during April and May, when the probability of catching pre-spawn males – and therefore detecting intersex condition – would be greatest. Testicular tissue samples were processed by the Veterinary Diagnostic Lab at the University of Illinois-Urbana Champaign.
Intersex condition was detected in 21 of 51 (41%) male largemouth bass collected during 2014. Interestingly, intersex males did not differ from normal males in length, weight, age, gonadal/somatic and hepatic/somatic indices of condition. Although no discernable differences in these metrics were observed between intersex and normal males, there could still be behavioral or reproductive differences that went undetected in this pilot study. A peer-reviewed manuscript of these results has been accepted, and is available in the July 2016 edition of American Midland Naturalist. As a follow-up to this 2014 pilot study, current INHS biologists have expanded the spatial and taxonomic scope of the study during 2015-16 to include collections of bluegill and black crappie at riverine sampling locations extending over 100 km downstream of the initial collection sites sampled in 2014.


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Modern Healthcare has ranked B. E. Smith #1 in its "Top Healthcare Executive Search Firms" for the second year in a row.
Clinical Pharmacist for Critical Care/Progressive Care (Varied Shifts)
Permanent Clinical Pharmacist in Arkansas
Emergency Department Clinical Pharmacist $10,000 Sign-On Bonus Available
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Our success is the result of meaningful patient relationships and exceptional pharmacy teams!
Safeway, a division of Albertsons Safeway Inc., is an innovative industry leader in community pharmacy practice.
Our pharmacists are part of a community where they know their patients by name.
Safeway, a division of Albertsons Companies, is an innovative industry leader in community pharmacy practice.
Centene Corporation is a Fortune 500 managed care enterprise that serves more than 11 million members across the United States.
Under the direction of the District Pharmacy Manager, the Pharmacy Manager is responsible for the financial performance of the pharmacy and acts as pharmacist in charge.
The Pharmacist works under the direction of the Pharmacy Manager to assist in pharmacy operations by supporting the mission, vision, and values of Safeway, Inc.
This temporary district pharmacy manager role is to efficiently oversee the operations of the pharmacies within the Denver Division.


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Utah Jazz notebook: Good price, but will team take Karl Malone up on his coaching offer?
Scott G Winterton, Deseret News
SALT LAKE CITY — Karl Malone is already The Mailman and a Hall of Famer.
If he gets his way, the former Utah Jazz star will be go by another description: assistant coach.
Malone doesn't want to replace any of Tyrone Corbin's current full-time helpers, but he wouldn't mind finding a spot next to Sidney Lowe, Jeff Hornacek, Michael Sanders and Brad Jones.
"All they've got to do is call me," Malone said during an interview with ESPN 700's Hans Olsen and James Rust on Wednesday afternoon. "I'll work with the big men for free for a while until it work."
This isn't the first time Malone has offered his coaching services to the organization he helped turn into a powerhouse and a two-time NBA Finals squad during his 18 years in Utah.
"I'm saying it again. Ain't nobody took me up on it," Malone said. "Maybe they don't want to hear me."
So, Coach?
Any room on the staff or a role for a legendary big man who dearly wants to do some pro bono work?
"Who knows? Who knows?" Corbin said. "We are where we are right now, but who knows what will happen."
Malone said he wouldn't necessarily want a full-time gig and he has no desire to travel with the team every game.
"But look. We can start off and see if it working," Malone said. "If not, I'll be the first one to say, 'Guys, it ain't working.' And they don't have to say nothing."
Malone has no doubt if the Jazz's former coach was still in charge — or is again elsewhere someday — he'd be in a gym teaching bigs how it's done.
"If coach (Jerry) Sloan ever got another coaching job, I would be with him sometime," he said. "End of story."
And, yes, he told Olsen and Rust he's not just popping off for fun about this idea.
"I'm being dead serious about this," Malone said. "I don't want no cameras around. I would be more than willing to come."
BIG REASON: One large factor Malone is interested: He's a big fan of the Jazz bigs.
"Utah Jazz is one of my favorite teams. I still have them doing damage," Malone said during the 25-minute interview. "Utah Jazz have a group of the best big men that's in the league. Go through any team (and compare)."
To that point, Malone said he wouldn't be in a rush to get rid of Al Jefferson or Paul Millsap.
"Everybody want to trade. But I got a news flash for you: They (trading partners) want a big man. If they (Jazz) want a good point guard, they're going to want your big guy," Malone said. "It's going to be very, very interesting with Paul and Al."
BIG TALENT: Malone wants to see what happens when injured Jazz guys return.
"All in all guys, don't start blowing up the team," he said in the radio interview. "If you don't have to get rid of a big guy, don't get rid of a big guy. You don't see a lot of them coming down the pike. But the fact of the matter is, we have talent on this team."
The sports talk-show hosts also asked Malone who he'd pick if he had to between Big Al and Malone's fellow Louisiana Tech product, Millsap. Malone grumbled and laughed about being put on the spot but then — you guessed — gave his opinion.
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South Beach Cultural Food and Walking Tour
Reviewed by: shelia m, July 2016
Very knowledgeable guide. Enjoyed each stop and taste along the way. An excellent introduction to Miami. I would highly recommend.
Reviewed by: Deborah A, July 2016
Carla was a great guide and gave us lots of information about all the lovely food we tasted as well as historic background of Art Deco district. A really nice way to be introduced to Miami Beach and I didn't have to worry about finding a restaurant for dinner! Larios made the best mojitos too extra, but worth it. Thank you!
Reviewed by: Jason H, July 2016
This tour was AWESOME!! Carla was our guide and did a fabulous job of showing us some of the best spots for Latin cuisine in the Art Deco Historic District. She was knowledgeable, passionate, and really made the tour a lot of fun. I would highly recommend this experience!!
Reviewed by: Thomas B, July 2016
This trip was awesome and our guide Lisa was the best guide we have ever had hands down.
Reviewed by: Kim.matt, June 2016
This was a great way to experience Miami food culture. The food was great our guide Lisa was very knowledgeable and enthusiastic which helps. Group of 12 of us. The food portions were reasonable and varied. The South Beach experience was memorable and I would highly recommend this tour.
Reviewed by: Evgenia L, USA, June 2016
As always, Viator selection of tours didn't disappoint! The guide, Lisa was outstanding - history of the South Beach mixed in with fun food selection at great local restaurants, not to mention super helpful suggestions about where we shall go on our own to eat. It was enough food to skip dinner that evening, so definitely worth the price. And we had so much fun walking and talking and trying out different places. Thank you!
Reviewed by: Nicola H, June 2016
Our guide, Carla, was absolutely fantastic. We were a small group of six and she made the tour friendly and informative with loads of facts about the art deco area. We tried foods we had never had before and we even went back to a couple of eateries in the followng days, so good was the food. The walking is well paced and the size of food portions are very substantial.


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Was in St Louis visiting colleges and decided to try the Ameristar for one night. Have been to Vegas many times along with a few non-LV casinos and I was pleasantly surprised at the Ameristar St Charles.
First of all, the hotel itself was really top notch. I had a Deluxe King Suite and it was surprisingly spacious, clean, nice bathroom amenities, two TVs. It was not very crowded so check in took no longer than 5 mins, bed was comfortable. Did not have much more interaction with staff but seemed courteous enough.
The casino itself was ok, Smoking always a problem but that's just how casinos are. Was glad to see there were more than just one or two crap tables since that is what I play mostly. Of course they try to replicate the Vegas casino feel but the attempts fall short without the size and scale of the Wynn, Bellagio, MGM etc. The dealers were all polite, efficient and friendly which is important for the overall gaming experience. For a local casino I was decently pleased overall and would probably stay here again if in the area.
- Official Description (provided by the hotel):
- The Ameristar Casino Resort Spa St. Charles is a 397-suite luxury hotel in the St. Louis area with many rooms containing floor-to-ceiling windows overlooking the Missouri River or the city below. This new, all-suite hotel features a 7,000-square-foot full-service spa with an indoor-outdoor pool. Have a great time at the region's largest casino, an action-packed night club or one of seven restaurants. You'll really get star treatment during your Ameristar vacation in St. Charles. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Expedia, Hotels.com, Hotwire, Orbitz, Travelocity, Agoda, Priceline, Odigeo and Jetsetter so you can book your Ameristar Casino Resort Spa St. Charles reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Ameristar Casino Resort Spa St. Charles Hotel Saint Charles
- Ameristar Hotel Saint Charles


text:
Originally posted on FOX Sports | Last updated 10/2/13
The UFC World Podcast with Gareth A Davies is now out... Episode No 8 https://itun.es/i6xC2Yc Michael Bisping describes in detail the eye injury which has forced his withdrawal from UFC FN 30. Bisping reveals he faced "possible blindness" in the eye if it wasn't operated on and was still prepared to fight until Dana White made the decision to pull him out. Davey Grant talks about his days in the TUF house, and how he had to look away as Ronda Rousey and Miesha Tate's feud went on... and on... and on. "They really do hate each other," he explains, on the eve of his contest due to air this week against Louis Fisette. Tom Wright, CEO of the UFC in Canada, Australia and New Zealand reveals how he has changed the Law in Canada, to make professional MMA legal, and how he is now battling politicians in Australia who are insisting on a ring, not an Octagon, be used for MMA fights. And joining Gareth in the studio, fresh from a bruising sparring session against each other as they prepar...
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text:
20% off sitewide. Don't keep your cart waiting. Use SCREAM20
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text:
Pure Buffalo Braided Bully Sticks Dog Treats
|Available Options
|Your Price
Experience the stampede of goodness in lean, 100% all-natural Pure Buffalo™ treats. As a healthier alternative to beef and great for maintaining a dog's dental health, Pure Buffalo is available in 22 varieties and sizes including bones, femur knuckles, braided bully sticks, tendons, and lung steaks.
bull pizzle
Reviewed by 5 customers
Our dogs love these treats, and not a lot of odor like some treats. Also, very clean looking treats, compared to some. Will definitely buy them again, when these are chewed up. Very lasting for the toy breeds we have.
- Please tell us your pet's breed:
- chihuahua, pekingese, pug
I bought two of these, one for each dog. The larger one was hollow and my Lab crunched thru it and finished it off in less than 20 minutes. Pretty sad showing for the price. The second is about as long as my hand from wrist to mid-knuckle and is still holding up. So I have mixed feelings about these. I won't buy them again tho. Not worth the risk of money over quality.
- Please tell us your pet's breed:
- 2 Labradors
We have two dogs, one who loves to chew, the other gets bored when alone and will dig if left outside, we think it's separation anxiety. We bought these knowing it will take longer to consume than a regular bully stick and they love those. They love these even more and we can give them to our dogs when we leave and the one who normally digs in the backyard seems to be satisfied by these and stays occupied with his braided bully stick and doesn't dig! I also never seen a better price than the one Petflow offers for these!
- Please tell us your pet's breed:
- Labrador & Shepard mix
Great treat for dogs.One of their favorite treats.
- Please tell us your pet's breed:
- German Shepherds
I have two rottweilers, and they generally consume any chew treats quickly. These kept them busy a bit longer, and they really enjoyed them.
- Please tell us your pet's breed:
- rottweiler
Pet lovers who bought this also bought...


text:
Links to recent pieces, blogs, and videos
South by Southwest 2015 panel on "Does sports = brain damage"?
– listing
– publicity
– video of panel discussion
Concussion pieces:
– On Brandi Chastain donating her brain to science
Magazine work
– AYP Magazine: "Releasing the Pressure"; "How to Collaborate"; "How to mentor"
– Signature Magazine, cover story on Multi-Channel business communication strategy, Jan/Feb. 2014.
New York Times work:
– Oakland University's unsized star, Kay Felder, making big waves
– Neurology is on the football sidelines at Michigan and Michigan State.
– Grosse Pointe Woods-Shores Little League makes it big.
– U.S. figure skating champion Alissa Czisny's comeback.
– Exclusive story with Ford scion and chair of the board Bill Ford Jr. and Little Caesar's/Wings/Tigers executive Chris Illitch both loving to play hockey. And they're actually not bad.
– Story on how the CBC is missing from the 2010 Olympics.
– Fox Sports Detroit.com: columns on the Detroit Pistons
– Awesome Aussie snowboarder and 2010 Olympic gold medalist Torah Bright. January 2010
– Snowboarding Olympians training in Northern Michigan. January 2010
– U.S. ice dance champions Meryl Davis and Charlie White did things their way. January 2010
– The New York Times Olympic blog: I contribute from time to time.
espnW work:
– A quick link to my archive.
– A feature on Japanese star player Homare Sawa.
– A column on what winning the World Cup could mean to the U.S.
– Feature on English goalie Karen Bardsley, who was raised in California.
Video diaries I shot:
– on Dresden.
– on the German train system.
Other publications:
– A fun story on the Michigan Wolverines Meryl Davis and Charlie White and their love of UM. January 2010
– My humorous opinion piece on NPR.com
– A series on concussions in hockey, and how Jason Botterill survived his injury. December 2009
– A story for the U.S. Olympic Committee about speed skating hopeful JR Celski. November 2009
– Story in M Magazine, published by the University of Michigan, on the father-son coaching duo of John and Patrick Beilein. November 2009
– A story for the U.S. Olympic Committee about former Great Lakes sailor Anna Tunnicliffe. Aug 2009
– Me and Dickie V., a fully multi-media experience. Here's the video. March 2009
– Some NBA playoff goodness, from the 2008 Pistons-Celtics series.
– Acting as AWSM spokesperson: A Sports Illustrated article on female sportswriters.
– A column about female athletes doing good things: Sheila Taormina and company.
– A story in USA Today on the Pistons: Flip Saunders intro'd as coach.


text:
USA Truck is looking for Class A CDL Company Truck Drivers that will be hauling 100% No-Touch Freight in dry vans. Drivers will enjoy a great compensation plan that is based on experience, plus benefits and home every other weekend! Trucks are assigned to drivers (no slip seating) and there is a rider and pet policy too. Apply Today using IntelliApp ---- it auto populates for most of our drivers: ...
Get jobs by email for this search
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text:
Walt Disney World Marathon
We'll be back at Walt Disney World in January 2017 running four races in four days. Join us!
When things go wrong during a race for which you've trained months, it's not worth making them worse.
48.6 miles in four days is supposed to be tough, but not this tough.
RW Challengers from around the world ran four races in four days—for a total of 48.6 miles—with the Runner's World editors.
Cedric King finishes his fourth marathon since losing limbs in Afghanistan.
When completing 48.6 miles in a weekend isn't the lasting memory.
Married couple will race at the Walt Disney World Half Marathon this weekend.
Runner's World staffer and Disney fanatic Lori Adams shares her best advice for spectators and runners.
Four races in four days. That sounds like a lot of luggage.
What you eat and drink during your weekend of races can impact your performance.
After 91 days of training for the Dopey Challenge, I've learned a few things.


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Take one random floating object, attach a radio beacon so you can find it again, drop it in the ocean and return later with a giant net and scoop up everything in sight. This is how much of the world's tuna is caught - using fish aggregating devices (FADs) with purse seine nets and it's a recipe for disaster. New Zealand's five big brands of canned tuna have committed to phase this destructive fishing method out of their supply chains.
This footage was given to Greenpeace by someone inside the tuna fishing industry who'd had enough of the destruction.
Help your cat join the CATavist movement against #badtuna at http://www.catsvsbadtuna.org and take action yourself at http://www.greenpeace.org/badtuna
The fishing communities of Kiribati, a Pacific nation comprising 33 small islands, speak about their loss of livelihood and erosion of their culture at the hands of destructive and oversized fishing vessels operating under European flags,...
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text: Jim Morrison May Finally Get Pardoned for Showing His PenisJim Newell11/08/10 5:07pmFiled to: PardonsCharlie CristJim Morrisonrock and rollthe doorsFloridaFb46EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalink Florida Gov. Charlie Crist, who will be unemployed in two months, may pardon the late Doors frontman Jim Morrison over a famous 1969 indecent exposure charge. (He drunkenly waved his penis around, allegedly.) Finally, hippies have something to cheer for.Reply46 repliesLeave a reply


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Category:Mohammad Khatami
From Wikipedia, the free encyclopedia
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The main article for this category is Mohammad Khatami.
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Wednesday, March 30, 2011
this one's for you
5 comments:
Well I don't know what the other 49 shots looked like but this one is stunning. You look healthy and beautiful.ReplyDelete
you look fantastic! almost there!ReplyDelete
ADORABLE...you look absolutely gorgeous! Wish I looked that chic pregnant, but at the time my go to outfit was a pair of navy cord overalls with a white t-shirt...I looked like a complete dork. Hope all is well! xx DanielleReplyDelete
You look great! Can't wait to keep updated to hear when the littlest moss makes his debut! Now go put your feet up! *AmyReplyDelete
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So much fun this week! Can't wait for the WILD CARD round at The Basement Theatre on Saturday, Apr 9 at 8pm! Check goldstar for cheap tix!!!
Jamie Ward is hilarious!!! Always fresh material and always trying different approaches to comedy! You should see him improvise too... he's hilarious on stage at The Basement in their improv shows!!!
DANCE TRUCK is AMAZING!!! They were at The Basement Theatre during Spontaneous COmbustion Atlanta... they were so cool! The dances were so evocative and engaging! I can't wait to see them again! Especially at MODA... cool locale for them!
I've seen the best comics here! And it's so cool to be in such an intimate setting. Each show has a theme, so it's fun to see the different comedians each week!
There's a monthly showcase where you vote for the best comics who get to feature the next month.
and now they have a Comedy Battle... the comics get a crazy topic 1 week before and have to write new material on that topic for the battle show.. then the winners get another topic that night, and then the winners of that round just go head to head to be funny off the cuff!
a great place for comedy!
We LOVE Shalewa - down in The Basement! She has played here a few times and always brings the house down!
hilarious! these guys are so much fun and so talented! I've seen several shows and it's always a laugh riot! They even bring people up on stage to be in the shows!
A coworker had her birthday party there and it was a blast! The whole show was based on her.
This is a definite MUST-DO... a cheap and awesome date night!!!
Bring Cash... they only take cash at the box office and bar... and they pour AWESOME drinks for CHEAP!!!


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ROCKVILLE, Md.—Choice Hotels International and Meyer Jabara Hotels announce the opening of the Cambria Hotel & Suites White Plains – Downtown. "The opening of the Cambria Hotel & Suites White Plains is an integral part to our continued expansion into ...Read More »
Author Archives: Lodging StaffFeed Subscription
Hilton to Sell Waldorf Astoria New York for $1.95 Billion
Hilton Worldwide Holdings Inc. has agreed to sell the Waldorf Astoria New York to Anbang Insurance Group Co. for $1.95 billion. As part of this long-term strategic partnership, Hilton Worldwide will continue to manage the property for the next 100 ...Read More »
G6 Hospitality Accelerates Franchise Growth
DALLAS—G6 Hospitality concluded its annual brand convention last week at the Hilton Hawaiian Village Waikiki Beach Resort in Hawaii by emphasizing three major initiatives: completing all Phoenix-design renovations; launching a new digital ecosystem; and opening new properties in key international ...Read More »
Marriott Fined $600,000 for Blocking Consumer WiFi Networks
Marriott International was fined $600,000 by the U.S. Federal Communications Commission for allegedly blocking customers from using mobile hotspots at the Gaylord Opryland Resort & Convention Center in Nashville, Tenn., and charging for access to the hotel's wireless network. According to ...Read More »
Loews Santa Monica Beach Hotel Appoints Jason Prendergast Executive Chef
SANTA MONICA, Calif.—Loews Santa Monica Beach Hotel has announced the appointment of Jason Prendergast as executive chef. A graduate of Culinary Institute of America in New York, Prendergast oversees all culinary operations at the hotel, including Ocean & Vine, Blue ...Read More »
IHG Launches Training Program for F&B Teams
InterContinental Hotels Group has launched a training program for the food and beverage teams in its hotels across the Asia, Middle East, and Africa (AMEA) and Greater China regions. The program, initially rolled out with more than 10 courses incorporating ...Read More »


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PORTLAND, Ore. -- Fred Meyer Inc. here has stepped up advertising of its everyday-low-price strategy for groceries, according to observers. In recent weeks, Fred Meyer print ads have urged consumers to compare its grocery prices with those at rival chains. The ads have also encouraged consumers to shop at other stores for specials but to come to Fred Meyer for their everyday supermarket needs. "They've been picking up media and print ads fairly aggressively in selected markets," especially ...
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- Score:
- rated 4.40 / 5 stars
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- metalgear
Credits & Info
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If you liked this, check these out!
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Author Comments
Rated 5 / 5 stars
That was awesome
I loved every second of it... Priceless face in the background.
Rated 4.5 / 5 stars
it was good
Rated 5 / 5 stars
Otacon hitting the bookcase when he realized Snake arrived was priceless. I guess Otacon has to learn to hide IN (not on) the box BEFORE he is seen
Rated 4.5 / 5 stars
well done
Otacon looks awsome same with snake. my favorite part is when Otacon falls in the box. Great job you draw really good
Rated 4.5 / 5 stars
Awww x3
I've never even played any of the games and I find this really cute XD maybe I'm just infected with a fatal case of the slashing virus, who knows?
Actually... I know, and that IS the reason. xD Aww poor Otacon :'3


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Translation of "junior high school" - English-Turkish dictionary
(Translation of "junior high school" from the Cambridge Learner's Dictionary English-Turkish © Cambridge University Press)
Get a quick, free translation!
- June noun
- jungle noun
- junior adjective
- junior college noun
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- junk food noun
- junk mail noun
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Word of the Day
a piece of cloth with a pattern or picture that is created by sewing or weaving different coloured threads onto a special type of strong cloth


text:
Department of Teaching and Learning
Ed.D. University of San Diego, Leadership Studies (Specialization: Character Education)
M.A. University of Hawai'i, East Asian Languages and Literature: Linguistics, Second/Foreign Language Pedagogy
B.A. University of Hawai'i, East Asian Languages and Literature: Japanese; Minor: Comparative Religion
USD Teaching and Learning Department
Sarina Chugani Molina currently serves as Assistant Professor in the Department of Learning and Teaching. She brings with her experiences of teaching Japanese language and culture to children and English as a foreign and second language to children overseas and adults at the community college and university level in the United States. She has taught multilevel English learners at the noncredit sector in Palomar College and was a credit instructor for the academic reading and writing courses at MiraCosta College. She also serves as the program director and trains teachers internationally in TESOL, a program she designed for the English Language Academy at the University of San Diego. She teaches "Introduction to Second Language Acquisition Theories," "Introduction to Linguistics and Linguistic Analysis," "Methods of Teaching Literacy for ESL," "Practicum in TESOL," and "Graduate Capstone Seminar (research)" courses in the M.Ed. in Literacy, Culture and TESOL program in the Learning and Teaching department, School of Leadership and Education Sciences at the University of San Diego. Her research interests include Teaching English as and International Language and TESOL teacher development, particularly as it relates to developing mindful, reflective practitioners.


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Real news. Real funny.
Real news. Real funny.
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The Walt Disney Family Museum Blog
Jul 8, 2016
From Sketch Artist to Animator: Inspecting Some Pieces by Retta Scott
Though the Walt Disney Family Museum offers exciting new displays and stories in each special exhibition that opens, there are also delightful new discoveries to be found in the main museum galleries when case rotations are made. Let's take a look at one of the latest rotations, in the case dedicated to one of Walt's most distinctive feature films, Bambi (1942).
Jul 2, 2016
In the Pool Hall with Three Disney Masters: A Closer Look at one of Pinocchio's Funniest Scenes
Being an epic of animated cinema, Pinocchio (1940) is ripe with moments of artistic beauty and raucously fun entertainment. The scene in the fabled pool hall, is a stand out for its combined mastery of the animated art form, thanks in particular to three of Walt's best animators.
Jun 29, 2016
A Whale of a Tale: An Ode to Monstro
In accordance with Shark Week and our latest special exhibition, Wish Upon A Star: The Art of Pinocchio, Monstro, the terrifying giant sperm whale that consumes Pinocchio, Geppetto, Figaro, and Cleo before sneezing them back out because Pinocchio, a character made entirely of wood, thought it prudent to start a bonfire.
Jun 17, 2016
"The Best Education I Ever Had": Mel Shaw at The Walt Disney Studios
Mel Shaw's life as an all-out renaissance man produced a near overwhelming amount of artistic creations, from painting to sculpture to sketches and pastels, many of which are exhibited in Mel Shaw: An Animator on Horseback at The Walt Disney Family Museum. The fascinating works on display include a selection of Shaw's work when he was working for Walt during the Studios' golden age.
Jun 15, 2016
Growing Up Disney
In honor of Father's Day, we share the stories told by Diane Disney Miller about her father, Walt Disney. In 1956, Diane sat down with journalist Peter Martin where she spoke candidly about what it was like to be Walt's daughter.
Jun 8, 2016
Paving the Way for Success: The Importance of Pinocchio
What makes Pinocchio such an important film in the Disney canon? Beyond the laudable artistic merits of the film itself, Pinocchio represents Walt Disney's greatest animated achievement.
Jun 3, 2016
Happy Birthday Jimmy Macdonald!
May 19th would have been Jimmy's 110th birthday. We invite you to celebrate Walt's chosen substitute by making some noise, Jimmy Macdonald-sound effects style!
May 26, 2016
John Canemaker Talks Tytla and Kahl and Pinocchio
On Saturday, May 21, 2016, John Canemaker, guest curator of Wish Upon a Star: The Art of Pinocchio, took guests on an in-depth exploration into the works of two great animation legends: Vladimir Tytla and Milt Kahl. Canemaker discussed Disney's extraordinary film, Pinocchio.
Oct 7, 2015
Remembering Kevin Corcoran (1949–2015)
"I am saddened to hear about the passing of Kevin Corcoran," said Miller. "My thoughts go out to Laura and his family. He was a great man and I will miss him."
Sep 16, 2015
American Experience: Walt Disney
WGBH's American Experience: Walt Disney, which aired on PBS this week, was created independently of The Walt Disney Family Museum. Here are our thoughts on the program.


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Spicy Melon Salad
Whisk 1 tablespoon each brown sugar and vegetable oil, 1/2 tablespoon fish sauce, 1 teaspoon Sriracha and the zest and juice of 1 lime. Drizzle over 1/2 each sliced cantaloupe and honeydew melon. Top with 2 tablespoons each chopped peanuts, cilantro and mint.
Photograph by Antonis Achilleos
Recipe courtesy of Food Network Magazine
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Melon Salad with Spiced Almonds
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Photographic line drawing of new york architecture
Credit: Michael Duva
Creative #: 167651861
License type: Rights-managedRights-managed products are licensed with restrictions on usage, such as limitations on size, placement, duration of use and geographic distribution. You will be asked to submit information concerning your intended use of the product, which will determine the scope of usage rights granted.
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Recently constructed and featuring a host of modern amenities, the residences at 9 Gardner Terrace are located on a quiet cul-de-sac that is just one block for the intersection of Commonwealth Avenue and Brighton Avenue in Allston's Packard's Corner neighborhood. Boston University, Star Supermarket, the MBTA Green Line (B), and Commonwealth Sports Club are all within very close proximity.
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Until you clear an area boss, the area is considered 'active' and that's what opens it up for invasions. That's also what allows you to gain humanity by killing the enemies in that area. Killing the boss 'clears' the area, so it's now safe. That allows players to be safe from invasions after successfully clearing an area. It's pretty obviously a balancing move and works pretty well.You seem incredibly ignorant of an incredibly large number of things. I recommend using the wiki to gain some basic understanding of how the game and community both actually work.
If there's no boss fog there's no way for the host to escape unwanted invasions. Its how the game is designed, deal with it?
You are not allowed to request a sticky.