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Dos and Don'ts of Pet Boarding »
Just like the planning that went into your vacation, there is planning needed before boarding your pet. Here are some dos and don'ts to help make the process a little easier.
Serving the Grand Rapids area.
3242 Leonard St NW, Grand Rapids, MI 49534
7750 Clyde Park Ave SW, Byron Center, MI 49315
9030 N Rodgers Ct SE, Caledonia, MI 49316
2883 E Paris Ave SE, Grand Rapids, MI 49512
2330 44th St SW, Wyoming, MI 49519
680 Plymouth Ave NEGrand Rapids, MI 49505
Best place in all of Grand Rapids to take your pet. I saddly had to put my very sick 18 month old kitten to sleep because of his illness. They treat…
3130 Breton Rd SEGrand Rapids, MI 49512
From Business: * New Patients Welcome Please Contact Us for Additional Information.
3012 Shaffer Ave SEGrand Rapids, MI 49512
I have mixed feelings about this place. While the vets are nice friendly and knowledgeable the place has always been pricey and nosw they try to pus…
2883 E Paris Ave SEGrand Rapids, MI 49512
Staff and Doctors go above and beyond the call of duty to care for our dogs.
3242 Leonard St NWGrand Rapids, MI 49534
After using many veterinarians around Kent County, I have to say that for everyday veterinary care, problem solving, and treatment, no clinic can ma…
2883 E Paris Ave SEGrand Rapids, MI 49512
133 28th St SEGrand Rapids, MI 49548
knowledgeable about all my exotic pets. Appointments available quickly.
4769 Cascade Rd SEGrand Rapids, MI 49546
Dr Stasiak is a very friendly, honest vet!
3215 Breton Rd SEGrand Rapids, MI 49512
Friendly and 2 locations! I love it!
3166 29th St SEGrand Rapids, MI 49512
This clinic may be a bit high priced, but it's well worth the extra cost to me to make sure my pet gets the VERY best care. The entire clinic is ver…
1527 Michigan St NEGrand Rapids, MI 49503
4547 Cascade Rd SEGrand Rapids, MI 49546
Ronnie has suffered for many years with a hereditary disease that causes awful nerve pain, twitching and crippling headaches. We took him to Dr. Rac…
1852 Breton Rd SEGrand Rapids, MI 49506
4609 Plainfield Ave NEGrand Rapids, MI 49525
I just moved to the area and I found a great vet hospital in Plainfield Pet. I was able to schedule my appointment, after chatting with the staff o…
1538 44th St SEGrand Rapids, MI 49508
After trying four different veterinarian facilities around town my wife and I decided on The Animal Hospital of Kentwood. We are a multi-cat and one…
2665 5 Mile Rd NEGrand Rapids, MI 49525
The care at this clinic is great. The staff and doctors treat you and your cat with the utmost respect.
3425 Plainfield Ave NEGrand Rapids, MI 49525
This is the best place to take your Great Dane for ear cropping! No doubt about it they do a very unique, cool cut! The best that I have seen!
3425 Plainfield Ave NEGrand Rapids, MI 49525
1971 E Beltline Ave NE Ste 110Grand Rapids, MI 49525
Dr. Thellman was on the phone with us twice for not less than 15 minutes each time, and when we brought our pet there for examination, he took plent…
1350 Lake Dr SEGrand Rapids, MI 49506
3012 Shaffer Ave SEGrand Rapids, MI 49512
1425 Michigan St NE Suite FGrand Rapids, MI 49503
133 28th St SEGrand Rapids, MI 49548
1052 Wealthy St SEGrand Rapids, MI 49506
3425 Plainfield Ave NEGrand Rapids, MI 49525
3425 Plainfield Ave NEGrand Rapids, MI 49525
2665 5 Mile Rd NEGrand Rapids, MI 49525
6730 Cascade Rd SEGrand Rapids, MI 49546
1527 Michigan St NEGrand Rapids, MI 49503
133 28th St SEGrand Rapids, MI 49548
Just like the planning that went into your vacation, there is planning needed before boarding your pet. Here are some dos and don'ts to help make the process a little easier.
When getting a new pet, you may be concerned about whether pet insurance is right for you. Find out if you should work pet insuran…
Paying for your vet's veterinary costs can get tricky. Learn how to make the most of your vet visits and pay for your furry friend…
I was referred to this vet clinic by the owner of the company I work for. He gave them great reviews about his experiences with his personal pets as well as his office cat. I figured for as happy as he was I had to try them out and am I glad I did. They are so personable and caring, they make you feel like your pet is as important as one of their own. (As far as price, my feelings are this...Most of us don't work for free because we have to pay our bills some how, why should we expect a vet to be any different. They charge prices that are average for the industry in exchange for a very important and good quality service and that is perfect in my opinion) In the 9 years that I have been taking my pets there, I have also turned on many family and friends to this facility whom also now rant and rave about how great Plymouth Roads employees are. Some of us have gone through the loss of a pet from terminal illness problems to old age and never once has this facility held guilt over our heads to make an extra buck. They are so great about providing all options possible at all cost ranges and then supporting what we choose is best for our personal situation. We even got a sweet card from them after our office cat was put down from old age (I mean really old age!) 8 Years later, that extra step that they took to go above and beyond still sticks in my head. Now that is customer service with a heart! I do understand that others have comment on a bad experience(s) that they have had. I can't comment on that because I don't know their personal situation but I can say for myself and all 10 other people that I personally know that take their pets there, we couldn't be happier. Plymouth Road Vet is a place that is very compassionate, caring, clean and over all and A+ in our book. I hope this review helps you and I suggest that you give them a try, I'd bet you'll love them like we do!
After trying four different veterinarian facilities around town my wife and I decided on The Animal Hospital of Kentwood. We are a multi-cat and one dog family. Of course we love our pets and take their care and well being as seriously as we would our own. We have found the Doctors and the entire staff to be very helpful, friendly, compassionate and knowledgeable. These folks really do live up to their mission statement. We have a kitty that had to go in a few months back. He was on an IV and had to stay for over a week. Every day after work we would stop in and they would let us see him. It was just like you would visit someone in a hospital! It was a warm and loving experience. He recovered, got to come home and is doing fine. We have also adopted a baby kitten from their cat adoption program (she's a sweetie!). We are glad to have found our "Veterinarian Family" and we would recommend The Animal Hospital of Kentwood to anyone seeking a Veterinarian to care for their beloved pets. C&D, Cutlerville Mi
I would like to thank Dr. Happel and the staff at Eastown Veterinary Clinic for the amazing veterinary care they provide to my personal cats/dogs and rescue/foster pups. They are extremely knowledgeable regarding preventative and diagnostic veterinary medicine. I have experienced veterinary care in 3 others states; my animals have received the best care from Eastown Veterinary Clinic. I am an extremely particular cat/dog owner and want answers to all my questions. Dr. Happel is amazing at applying her scientific knowledge. I have friends who travel 1.75 hours for veterinary care at Eastown Veterinary Clinic. Love Eastown Veterinary Clinic!! Thomas G.
Choosing the right vet for your pet can be tough. After all, your furry friend can't tell you how he or she feels about the doctor. Even though you're not the one treated by the vet, whoever your animal sees is obviously your decision. Since many veterinary diseases and injuries can turn into emergencies very quickly, it's important to have a go-to vet. This way, you can ensure you'll know whom to see when your animal needs care.
Speak to your friends and family about vets who've treated their pets. You can even talk to your groomer or an animal shelter worker for referrals. When you visit the clinics you've been referred to, check that the facility is clean, animals are separated and the staff is calm and courteous. Not all clinics are American Animal Hospital Association accredited. This accreditation isn't a legal necessity, though a clinic that's AAHA-accredited is guaranteed to offer high-quality medical care. To receive accreditation, the clinic has to meet the AAHA's standards in the areas of facility, equipment and quality care.
If you're looking for a specialist, you want to make sure he or she is board-certified to practice in that specific area of animal medicine. You'll want to make sure your vet is also convenient to visit, so there are factors to take into account.
The type of animal you own should play a part in which vet you choose as well. While your options are vast if you have a dog or cat, you may have to visit an avian clinic for your bird or an exotics clinic for your snake.
Just as there are many types of doctors, there are many types of vets. Some focus on livestock or house pets, while others may specialize in dentistry or surgery. They may work in a veterinary clinic or zoo, working specifically with the animals housed there, or travel to farms to work with livestock. Since horse racing and other equestrian activities are so popular, some vets are trained to work just with horses.
Diseases, like malaria and yellow fever are also transmitted through animals. Some vets have insight to diseases that affect both humans and animals. Vets have contributed to the treatment and cure of many diseases that plagued both humans and their furry friends.
Government agencies employ veterinarians as well. When an animal comes from a foreign land, these vets quarantine them and check for any diseases that may be present in an effort to control new diseases that can be brought into the country. Other Specific types of vets include:
A vet assistant works alongside the veterinarian and helps out around the clinic. In some cases, they may assist vets in surgery or restrain struggling animals during tests or lab work. The everyday duties of a veterinary assistant include; monitoring and caring for animals after surgery, keeping medical records, cleaning animals' teeth, feeding and bathing them, cleaning cages, sterilizing surgical equipment, giving animals medication, collecting samples for testing and performing laboratory tests, and offering grief counseling to pet owners.
It's a good idea to bring your pet to the vet regularly. This way, he or she becomes familiar and comfortable with the care providers, and you can stay on top of your pet's preventative care. If the animal is small enough, bring it to the office in a carrier. Just as you visit the doctor for a yearly check up, you should bring in your pet for regular check ups as well. During a routine veterinary visit, the vet will probably begin by asking you if there have been any changes in your pet's behavior or habits.
The vet will then take your pet's vitals, like weight, temperature, pulse and respiration rate, and perform a physical examination of the pet. During a physical exam, the vet checks the abdomen for swollen organs, and the legs, feet and joints for any potential problems. Depending on the age, breed or condition of your pet, your veterinarian may also check the eyes, ears and mouth.
When your vet conducts a full body examination, he or she will check out your pet's coat and skin, noting any hair loss, itchy spots or lumps. Keep note of your animal's shedding habits so you can let the vet know if anything seems abnormal. The vet will check for parasites, fleas, ticks, mites and heartworms as well.
Vaccinations are also important to your pet, especially if you have a cat or a dog, and your vet will suggest that you make sure they're current. Keeping up to date with vaccinations can prevent your furry friend from getting distemper, rabies, hepatitis and lyme disease. Some vaccinations last longer than others, so speak to your doctor about staying caught up with your animal's shots.
Just like your own health insurance, you want to make sure your animal is covered before he or she needs veterinary services. Some common animal surgeries can cost thousands of dollars, and you don't want to end up having to foot a surprise bill that costs more than your paycheck.
There's no set price for pet health insurance. Costs can depend on factors such as where you live, the age and breed of your pet, and how much coverage you want. Before you take out a pet insurance policy, you'll want to meet with your vet to go over what he or she thinks your animal should be covered for. Many vets believe that you should make sure cancer, chronic disease, hereditary and congenital disease, and common breed-related medical conditions are all addressed in your policy.
Some pet owners can't afford insurance for their pet, so there are other options to make paying for surprise pet visits as easy as possible. Some pet stores have wellness plans - which tend to be much cheaper than an insurance policy - that offer shots, check ups, screenings and discounts on various procedures your pet may need. A lot of veterinary offices offer payment plans for pricey procedures as well, as long as you have decent credit history. For a last-ditch option, there are even privately funded organizations that offer pet owners financial aid for their pet's treatments.

subset: commoncrawl


text:
September 2011
BS ISO 10845-2:2011 Construction procurement. Formatting and compilation of procurementBS ISO 10845-2 establishes, in respect of supply, services and engineering and construction works contracts, at both main and subcontract levels,
Procurement documents are required in order to:
A uniform format for the compilation of calls for expressions of interest and tender and contract documents provides the platform for the standardization of the component documents and improved communications between those engaged in the procurement process.
Contents for BS ISO 10845-2 includes:
ScopeTerms and definitionsGeneral requirements for procurement documents
Component documents
Notice and invitation to submit expressions of interest
Tender documents
Contract documents
Compiling tender documents
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text: Joss Whedon explains why even The Avengers's outtakes matter, plus Nicolas Cage's skull will explode... IN 3D!!!Alasdair Wilkins8/26/10 9:00amFiled to: morning spoilersThe AvengersTransformers 3twilightBreaking dawnthe hobbitnever let me goGhost RiderGhost Rider 2devilChuckWarehouse 13Three inchesVsmallville120EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalink Joss Whedon writes scenes that he knows will never make the finished Avengers — but they're still important. James Marsters will lead Syfy's team of unimpressive superheroes. Plus Ghost Rider 2, V, Smallville, Never Let Me Go and The Hobbit. The Avengers: The Sydney Morning Herald has an excellent overview of the trials and tribulations of Joss Whedon's career, but at least it has a happy ending (for now), what with him directing The Avengers and all. He says that this is the job "I've waited for my whole life" and shares some details on his writing process for the film: Advertisement "Right now I'm working on a movie that's got enormous stipulations and is going to be changing and fluid every second. I've come up with dozens of scenes and lines and exchanges and monologues that I adore that are not going to be in it. But while I'm writing them they feed me, excite me and they ultimately inform the character. It all goes in." [The Sydney Morning Herald] Transformers 3: Sponsored An ABC Chicago news report notes that Kevin Dunn and Julie White, who play Sam's parents, are in town for filming, which suggests they feature at the end of the movie in some capacity. [TFLAMB] Producer Lorenzo di Bonaventura explained why they've spent so much time filming in Chicago: "It's funny, we keep extending it here because we're having such a good time, to tell you the truth. We were supposed to leave a few days ago, actually, and we kept extending it. Right now, around September 1st, but if we have any say in it, we'll keep staying here." Not sure if that means they're simply doing a lot of additional photography, shooting more scenes in Chicago than they had originally intended and having the city stand in for other locations, or even adding new stuff (probably the first, but you never know), but at least it explains why I thought they had finished Chicago filming at least two or three times now. [Chicago Breaking News Center] Twilight - Breaking Dawn: Advertisement Summit Entertainment says they may abandon their plans to film part of the movie in Rio de Janeiro due to a recent hotel shootout in which 35 tourists were taken hostage. Brazilian officials are hoping to convince the filmmakers to stay. No word on where they might relocate to if they don't film scenes in Rio. [AFP] The Hobbit: No, there's no news, and no rumors, but there's rumors of news. (Or maybe it's news of rumors. No, no, rumors of news, definitely.) Peter Jackson recently said that MGM appears to be making good progress on sorting out its hellish bankruptcy fiasco, and there should be more certainty on The Hobbit soon. So...we might know something soon, possibly. [The Dominion Post] Never Let Me Go: Here are some artsy posters for the British cloning drama that stars Sally Sparrow, the new Spider-Man, and Keira Knightley. [CinemaBlend] [gallery 5622185] Ghost Rider 2: The sequel to the wildly unacclaimed 2007 movie teams Nicolas Cage and Crank masterminds Mark Neveldine and Brian Taylor in what will either be the awesomest or the stupidest movie ever made (or, hopefully, both). It turns out their insanity can't be contained to just two dimensions - the film will shoot in 3D. [Hot News] Chuck: This season will reportedly introduce us to two members of Casey's old Special Ops team. They will apparently have shades of Justin Long and John Hodgman's personae from the "I'm a Mac/I'm a PC" commercials. [TV Guide] Warehouse 13: Here's the promo for next week's episode, "Vendetta": [SpoilerTV] Click to view Three Inches: James Marsters has been cast as Troy, the leader of a team of people with entertainingly useless superpowers. (The main character Walter has the ability to move anything up to three inches with his mind, hence the title.) No word yet on what powers Troy will have, but I say they should just make him Captain John from Torchwood. Because really, has there ever been a more entertainingly useless character than Captain John? [Digital Spy] V: Elizabeth Mitchell discussed the upcoming season in a recent interview. She explained, with a look of relief that was maybe just a tad too obvious: "It's so much better. We kinda set the groundwork and...it's off and running, which I needed it to be. And it's more fun for me." She also explained that her character is a lot more intense this year, and does things that apparently left three men utterly shocked. (That's her metric for intensity by the way, not mine. I might need to start using it though.) [E! Online] And here are some set pics from the season premiere, "Red Rain": [SpoilerTV] [gallery 5622200] Smallville: According to executive producer Kelly Souders, there's a twist in the premiere that: "rocks [Clark's] world and sends him on a different trajectory... He realizes that he's not the guy that everybody looks to when they think of Superman, an inspirational totally pure-hearted guy. That's not who Clark Kent is right now, and that becomes very clear to him." [TV Guide] And here are some new photos from the set: [SpoilerTV] Additional reporting by Kelly Faircloth and Charlie Jane Anders.Reply120 repliesLeave a reply


text: Fake Bratpack Minimix Series - PART 1, Mixed by Emoh Instead by Fake Bratpack published on 2011-05-16T10:25:51Z "Darkness shrouds the grimy streets of Sydney city. The foul stench of the past lingers over the towns-people like a suffocating blanket. Broadcasted through every stereo, the Dark Lord of the Top 40 spread's his P!nk propaganda, corrupting the minds of the weak, and disturbing others. Nothing is left untouched by the filth –not even puppies. Plagued by Mylie Cyrus and Rebecca black, the city's inhabitants cry out for a saviour. No longer will they put their hands up for Detroit. They need something more. This city needs a hero. Meanwhile, back at FakeClub headquarters, four men dared to dream of a better world. They realised that with their powers combined they might just stand a chance. And almost like a divine intervention, the prayers of the people were answered. Through the power and might of It's Rain, Elmo is Dead, Jace Disgrace and Granger Danger, the Fake BratPack was formed. Banding together, this underground vigilante syndicate fights back to topple the Dark Lord's rule, and end his reign of terror once and for all"... 120 Tracks in under 20 minutes Yeah Bitches! Comment by Smokty genial 2015/06/06 10:08:44 +0000 Comment by DTG This mix is awesome, did any others ever get made 2014/11/15 14:08:09 +0000 Comment by Delroy ID all of these 2013/10/22 22:44:13 +0000 Comment by Delroy ID ID ID !! 2013/10/22 22:44:01 +0000 Comment by Delroy ID? 2013/10/22 22:43:11 +0000 Comment by Boundski loving this! 2013/02/07 02:15:15 +0000 Comment by JASTI what you mashing with style here?? 2012/10/31 05:58:05 +0000 Comment by Le Dupe This mix is mustard! 2012/10/25 13:02:13 +0000 Comment by Le Dupe @lombardo92: TC- Where's my money, mashed with something else 2012/10/25 12:48:51 +0000 Comment by Fakenswitch good job boys fucking love this mixtape 2011/11/22 23:39:18 +0000 Comment by MAINst track id ????????????????????????????????????????????????? 2011/05/28 11:03:32 +0000


text:
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Posted: Jul 22
The Rainbow Festival 2016, Playwrights
- Performs two weekends in September at the new 60-seat Roebuck Theater in Midtown NYC.
- Seeking submissions from: New York, NY
- Seeking submissions nationwide/worldwide
Seeking playwrights for the AlphaNYC Theater Company's first ever Rainbow Theater Festival. Producer states: "We are seeking plays written with at least one LGBT character and/or that deal with themes or causes important to the LGBT community. There is no charge to participate or submit a play. Plays must be ...
Posted: Jul 22
NY Kids Club, Weekend Special Events Performer
- Instructors are expected to be available Fri. (3-8 p.m.), Sat. (8 a.m.-6:30 p.m. or 12-8:30 p.m.), and Sun. (10 a.m.-6:30 p.m.) in NYC; weekday hours are also offered on an ongoing basis.
- Seeking submissions from: New York, NY
Seeking part-time teachers to be a part of the New York Kids Club's Weekends Special Events Team. The company has opportunities in all 12 of its children enrichment centers throughout New York City. Recruiting manager states: "The New York Kids Club is the premier children's enrichment center in New York. ...
Posted: Jul 22
- Shooting takes place August and September 2016 in Tinley Park, Mokena, and University Park, IL. Must be present for every assigned shoot date.
- Seeking submissions from: Tinley Park, IL; Orland Park, IL; Mokena, IL; Southwest Suburbs, IL
Casting for a short film "The Wild Cats," about an experienced Illinois lawyer who represents his long-time friend in a court case against a corrupt corporate CEO while he constantly tries to keep his two kids from getting into trouble.
Posted: Jul 22
- Rehearses in Sept.; shoot begins in Oct. in Baltimore, MD.
- Seeking submissions from: Baltimore, MD
Casting "Ok What Now?," an LGBT web series. Synopsis: "Ok What Now?" is about a girl from an Afghan family accepting her sexuality and culture together.
Posted: Jul 22
- Rehearses in Aug. and Sept.; performs in Oct. in Atlantic City, NJ.
- Seeking submissions from: Atlantic City, NJ
Casting "Rent," the musical. Synopsis: Artists living in early-'90s New York City deal with AIDS, poverty, art, and love all while trying to find meaning in their lives.
Posted: Jul 22
- Shoots Aug.-Dec. in the L.A. area.
- Seeking submissions from: North Hollywood, CA
Casting "Canaan Land," a film about a con man preacher who falls in love with a true believer and realizes the con isn't a game anymore.
Posted: Jul 22
- Rehearsals are generally scheduled for Sun. evenings; the rehearsal space is in Old Town, Chicago, IL.
- Seeking submissions from: Chicago, IL
Seeking female singer for Candy Town, a clown-faced musical act playing original material which draws stylistically from Kurt Weill, Tom Waits, and the Beatles. The presentation is very theatrical, with elements of vaudeville and burlesque
Posted: Jul 22
Princess & Mermaid Cast for Events & Parties
- Works weekends based on your availability in Orange County and L.A. Costume pick up in O.C.
- Seeking submissions from: Irvine, CA
Seeking cast members to play storybook princesses and swimming mermaids for events and parties.
Posted: Jul 22
Stronger Health Fitness, Personal Trainers
- Work dates TBD in the NYC area.
- Seeking submissions from: New York, NY
Seeking actors with a background in personal training to join Stronger Health Fitness's health and fitness team. Coordinator states: "We are excited to cast this role in 'real life' due to the overwhelming response we received casting our project. We are an integrated multidisciplinary comprehensive health and fitness company that ...
Posted: Jul 21
- Shoots in Aug. in L.A.
- Seeking submissions from: Los Angeles, CA
Casting "Cleansed," an independent web series. Synopsis: "Cleansed" follows a jaded L.A. thirty-something who goes on a magical juice cleanse.
Posted: Jul 21
- Shoots Aug. 6 in L.A.
- Seeking submissions from: Los Angeles, CA
Casting a teaser trailer for the short film "Papa."
Posted: Jul 21
- Shoot dates TBD.
- Seeking submissions from: Los Angeles, CA
- Seeking submissions nationwide/worldwide
Seeking people with best/worst stories for "Best/Worst Tale," a reality show featuring best/worst stories told by the people who lived them.


text:
|Publication number
|Publication type
|Application number
|Publication date
|May 22, 2007
|Filing date
|Nov 15, 2002
|Priority date
|Nov 15, 2002
|Fee status
|Also published as
|Publication number
|David J. Rakestraw, Don W. Arnold, Phillip H. Paul
|Original Assignee
|Eksigent Technologies, Llc
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (12), Non-Patent Citations (2), Referenced by (7), Classifications (27), Legal Events (6)
|External Links: USPTO, USPTO Assignment, Espacenet
Chemical analysis is very important for life sciences research, clinical diagnostics and a wide range of environmental and process monitoring. An important class of measurements are conducted on particles such as cells or beads. For most of these application it is desirable that the analysis tools and methods be accurate, fast, easy to use and low cost.
Flow cytometry can be used for analyzing particulate elements including cells and beads, including beads having attached thereto biological materials. Exemplary flow cytometry apparatus and methods are described in U.S. Pat. Nos. 5,747,349; 5,981,180; and 6,139,800, which are incorporated herein by reference. Flow cytometry has disadvantages. For example the particles to be analyzed need to positioned very steadily, and only one particle can be detected at a time. Thus particles need to move at a steady flow rate for accurate results, but also at very high rates for high sample throughput. To obtain accurate results under these conditions, flow cytometry utilizes sophisticated, high speed, expensive detection electronics, which results in a relatively large and expensive instrument.
Other techniques for analyzing particles are described in Brenner, S.; et al, "Gene Expression Analysis by Massively Parallel Signature Sequencing (MPSS) on Micro Bead Arrays" Nature Biotechnology (2000), 18, 630–634; and Kitamori, T.; et al, "Integration of an Immunosorbent Assay System: Analysis of Secretory Human Immunoglobulin A on Polystyrene Beads in a Microchip" Anal. Chem. (2000), 72, 1144–1147.
The techniques described in these publications suffer from one or more disadvantages, such as an inability to analyze more than a single particle at a time, uncontrolled positioning of particles making analysis difficult, overlapping of particles so that the one particle interferes with the analysis of another particular, the need for all particles to have substantially the same size, and low signal to noise ratios, thereby making analysis difficult.
Accordingly, there is a need for a system for processing particles that is rapid, accurate, low cost and capable of processing multiple particles simultaneously, and that does not require all the particles to be of the same size.
The present invention provides a system that satisfies this need. The system includes a processor, also referred to as an analyzer, which is used for processing a feed stream, also referred to as a sample, containing a liquid and a plurality of particulate elements. The analyzer comprises (i) a body, (ii) a process section, also referred to as an analysis section, in the body, (iii) an inlet conduit into the body, and typically (iv) an outlet conduit from the body. The inlet conduit is sufficiently large that the feed stream can flow there through into the process section. Similarly the outlet conduit is sufficiently large to remove analyzed sample from the analysis section.
The process section comprises positioning means for positioning the particles in the process section so that substantially all the particles are constrained in a predetermined location, when introduction of the feed stream into the process section is stopped. The predetermined location is well-defined. Preferably the particles are constrained so that there is substantially no overlap of the particles. The process section is contained in a plane, and the positioning means positions the particulate elements so there is substantially no overlap in the plane of the process section when introduction of the feed stream into the process section is stopped. The positioning means can be a plurality of particle retainers, such as pockets or conduits through a weir, sized to receive the particulate elements, each particle retainer being sized to receive only one particulate element at a time. The particle retainers only temporarily retain the particulate elements, and thus, are configured to allow a retained particulate element to be removed therefrom.
The positioning means can also include means for selectively forcing the particulate elements into the particle retainers. As an example, the particulate elements can include a magnet responsive element and the analyzer comprises a magnet for selectively forcing particulate elements into the particle retainer. Alternatively the particulate elements can be electrically charged and the analyzer can include an electric field generator for forcing the particulate elements into the particle retainers.
Preferably the positioning means comprises a positioning fluid for selectively forcing particulate elements, into the particle retainer, where the body has a positioning fluid inlet and a source of positioning fluid that is introduced to the positioning fluid inlet. In this version of the invention, the body can have positioning fluid outlet for removing positioning fluid from the process section. Preferably the process section has a structure such as a weir, a porous material, vias, or posts that prevents the particulate elements from leaving the process section through the positioning fluid outlet.
The particulate elements can be processed by any technique found in the art, as is appropriate for the specific particulate elements being processed, including analysis of particles by use of a light source for producing a signal. The light source provides light of a selected wavelength and to which the particulate elements respond by producing a signal. The particulate elements can have a label such as a fluorescent dye to produce a light signal. A detector is used to detect the signal from the particulate elements.
An analyzer according to the present invention can have multiple process sections, each being provided with its own sample inlet conduit and sample outlet so that a large numbers of particles can be analyzed simultaneously.
In a method according to present invention (a) a feed stream is introduced into the analyzer through the inlet conduit to flow into the process section; (b) a first set of particulate elements is positioned in a predetermined location such as in the particle retainers; and (c) analyzed therein. The analyzed particles are then released, and steps (a) through (c) are repeated with additional sets of particulate elements.
The analyzer can include a control system for introducing sample intermittently, in defined time intervals, and intermittently positioning the particulate elements at the predetermined location for analysis.
Thus, according to the present invention a large number of particulate elements can be processed at one time, where the particulate elements do not interfere with the analysis of each other, in a system that provides a high signal to noise ratio, and that is fast, inexpensive, and efficient.
These and other features, aspects and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:
The present invention provides a system for analyzing a plurality of particulate elements simultaneously, quickly, efficiently, and at low cost.
A portion of an analyzer 12 that exemplifies some of the features of the present invention is shown in more detail in
The analysis chamber 30 is elongated, having a longitudinal flow path and a longitudinal axis 39 as shown in
The analysis chamber 30 has particle retainers such as a pair of weirs 40 a and 40 b that extend parallel to the longitudinal axis 39 of the analysis chamber 30 and serve to define a flow channel 42 for the sample. The weirs 40 a and 40 b have opposed side walls 41 a and 41 b respectively. The weirs 40 a and 40 b are sized so that particles in the sample cannot pass over the weirs, although liquid in the sample and the positioning fluid can pass over the weirs. Thus, with reference to
The positioning fluid inlet 36 and outlet 38 are constructed to provide relatively uniform flow across the entire interface area where they contact the analysis chamber. The positioning fluid inlet and outlet include flow diffusers 45 which assure that positioning fluid flow is uniform across the entire interfacial area in contact with the analysis chamber. Each flow diffuser 45 is essentially an elongated channel defined by one of the weirs 40 and one of the analysis chamber side walls 31.
For ease of illustration, all of features of the analyzer, including the analysis chamber 30, the sample inlet conduit 32, the sample outlet conduit 34, the positioning fluid inlet conduit 36, the positioning fluid outlet conduit 38, the weirs 40, and the flow diffusers 45 are shown as formed only in the bottom 28 b of the body 28. Fabrication of analyzers with features in both the top 28 a and bottom 28 b of the body can also be accomplished with standard fabrication techniques.
As shown in
The analyzer 12 can be fabricated from many different materials, including silicon, glass, silicate, silicone, quartz and other ceramics, plastics, elastomers, and metals. When the particles are analyzed by a light source at a selected wavelength, a portion of the analyzer 12 proximate to the pockets needs to be substantially transparent to the incoming analysis light, and also to any light produced by the reaction of the particles to the incoming light. The term "substantially transparent" means that at least 60% of indirect light reaches the particles, and at least 40% of light from the particles passes through the analyzer.
The features in the analyzer body 28, such as the conduits, the analysis chamber and the pockets, can be prepared by many of known fabrication techniques, including machining, laser machining, injection molding, embossing, wet chemical etching, dry chemical etching, water jet machining, and bead jet abrasive machining.
The sample conduits 32 and 34 are sufficiently large that particles can freely flow there through. Generally the smallest dimension is at least 100% larger than the largest dimension of the particles. In an exemplary embodiment, the conduits are semicylindrical in shape, because they are formed only in the bottom 28 b of the body 28, and have a width of about 75 microns and a depth of about 50 microns. The advantages of small sized conduits include the ability to rapidly introduce and position particles with minimal amounts of fluid and to maximize the number of conduits in a single analyzer 12 to provide a high rate of particle analysis. However if the conduits are too small, it is possible for them to be clogged by large particles and/or create substantial resistance to flow which increases the requirement on the pumps 14, 15, 16, and 17.
The analysis chamber 30 typically has the same depth as the sample conduits 32 and 34, and a width of about 40 to about 100 microns. In a typical system, the analysis chamber has a depth of about 10 to about 50 microns.
As shown in
The pumps 14, 15, 16 and 17 can be electroosmotic pumps, also known as electrokinetic pumps. Electroosmotic pumps are advantageous because they can accurately introduce as low as 100 pico liters of fluid per cycle, with a precise control. Electrokinetic and electroosmotic pumps are described in U.S. Pat. Nos. 6,013,164; 6,277,257; 6,013,164; and 3,923,426, which are incorporated herein by reference. Other types of controlled pressure devices that can be used are syringe-type pumps, piezo-actuated diaphragm pumps, microbubble pumps, pneumatic pumps, peristaltic pumps or any other pump that can deliver controlled volumes of fluid as necessary to manipulate the particulate-laden suspension in the analysis device.
With reference to
Step 1—As shown in
Step 2—As shown in
Step 3—The positioned particles in the pockets 46 are analyzed.
Step 4—As shown in
The number of particles in the analysis chamber is preferably correlated with the number of pockets 46. To be sure that all the particles find a pocket and to minimize stacking of particles, i.e., more than one particle in a pocket, preferably the number of pockets is at least about 1.5 and can be up to about 3 times the number of particles in the chamber, also referred to as a process section. Thus as shown in
The whole procedure can be performed rapidly, in the order of 1 millisecond for analyzing as many as 5000 beads in a single analysis chamber.
The controller can be any properly programmed microprocessor.
The positioning fluid is a liquid, and needs to be selected so that it does not adversely interact with the particles. For most biological samples, appropriate saline conditions must be satisfied as necessary.
Other methods are available for positioning the particles against the weirs 40 a and 40 b and into the pockets 46. With appropriate selection of substrate materials and particle suspension fluids, as known to those knowledgeable in the art of electroosmosis, the particles can be positioned with electroosmotically-driven fluid flow by application of an appropriate electric field. Alternatively, for charged particles, electrophoretic techniques can be used, where electrodes are used to apply an electric field across the chamber. For situations where particles naturally do not have a sufficient charge for electrophoretic techniques, the particles can be provided with such a charge, which is easily done in a case of bead based analysis. An advantage of electrophoretic positioning is that pockets 46 can be on both weirs 40 a and 40 b , and particles having opposite electrical charge can be placed in opposing sets of pockets.
Another technique that can be used is magnetic positioning, where the particles have a magnetic responsive element, such as beads with an iron coating. By application of a magnetic field to the analysis chamber, either with a natural magnet, man-made magnet, or electromagnet, the magnetic responsive particles can be forced into pockets.
By the term "means for positioning" there is meant not only the positioning fluid, natural magnets, artificial magnets, electromagnetics, and electrical field generators specifically described, but also any structure presently existing or invented in the future effective for positioning particles in a predetermined location for processing.
The particles that are analyzed can be naturally occurring material such as cells, including red and white blood cells, and cell fragments. The particles can also be a material such as silica, aluminum, metal, ceramic, and polymers. The particles can be of any type that have been used in cytometry including polystyrene latex particles, acrylate or methacrylate derived particles, hydrogel polymer particles, polymerized micelle particles, particles produced by grinding cast film, particles produced by photopolymerization of aqueous emulsion, and particles produced by solvent casting as described in U.S. Pat. Nos. 4,302,166 and 4,162,284.
Beads for cytometry typically range from about 0.1 μm to about 50 μm, and more typically from about 1 μm to about 20 μm in diameter. Their density is typically from about 0.5 to about 2 grams per milliliter. The particles need not be of the same size.
Typically such polymer-based beads are coded with detectable labels. For example, beads can be coded with one or more fluorescent labels, and can have on their surface capture agents for capturing a target analyte in sample, resulting in a detectible signal from the bead, particle or labeling coating. Preferably the particles incorporates coding indicia which enable unambiguous identification of the particle type, and consequently enables the analysis system to assign measurement signals from the particle and any specific analyte with which the bead interacts. Particle labeling or coding can be accomplished by varying detectable particle properties such as intensity of fluorescence from fluorescent dyes associated with the particles; ratios of intensities of fluorescence from multiple fluorescent dyes associated with the particles, size, shape, reflectivity, and relative number of the particles, and combinations of any of the above characteristics integrated over the entire particle or the spatial distribution of such properties within each particle. A "detectable signal" can be the absence of light, i.e., a fluorophore could be quenched so that it gives off no light. It is also possible to code the particles by adding materials with other detectable properties such as magnetic materials. Additionally information about useful coding schemes may be found in Fulwyler, U.S. Pat. No. 4,499,052; Coulter Electronics, UK Patent No. 1,561,042; and Tripatzis, European Patent No. 126450.
The target analytes can be any of a large number of materials, including:
Once the particles are positioned, a variety of different light interrogation techniques can be used to identify the particles and to determine the response of the particles to the assay. For instance one or more dyes can be used, and different colors and/or different dye concentrations can be used for generating different codes from different particles. Interrogated properties can include, but are not limited to size, shape, morphology, reflectivity, fluorescence intensity, fluorescence wavelength, composition, and bound target analyte. Detection methods can be based on magnetic properties, radioactive properties, and electrochemical properties including electro chemiluminescence. For example, by patterning electrodes into the analysis chambers, voltage can be applied to the particles after they are parked in the pockets, and then it is possible to measure an electrochemical signal or electrochemically generated optical signal (i.e., through chemiluminescence) as detected with an optical detector.
Alternatively, as shown in
Among the detection devices 60 that can be used are CCD cameras, CID cameras, diode arrays, and photographic film.
The filters 58 can be used for taking images of different colors by changing filters or using a tunable filter or using a multitude of filters in parallel. This allows use of multiple, different wave light sources, and multiple, different wavelength labeled particles.
For each detection position defined by the pockets 46, the detector 60 collects a signal relating to one or more interrogated properties from the particles in the pockets. High throughput is achieved because multiple particles are analyzed simultaneously. The signal from the particles can be captured in a time frame in the order of milliseconds to seconds and the data can be analyzed while the sample is removed from the chamber and a new sample is positioned for analysis, which again can take place in the order of milliseconds. By analyzing thousands of particles simultaneously with this method, very high sample analysis rate can be achieved.
In some instances, a detection system may not be needed, in that a human can visually inspect the particles for distinguishing characteristic of interest.
As shown in
Instead of using pockets as the particle retainers, as shown in
In a dynamic system according to the present invention, assays can be conducted while the particles undergo dynamic change while they are positioned for detection in the chamber. For example the composition of fluid that is in contact with the particles can be altered to induce chemical or physical changes to the particles, any species bound to the particles or the binding interaction of the species and the particles. Through proper interrogation methods it is possible to carry out kinetic measurements by monitoring the particles and/or a bound species as a function of time.
With reference to
Step 1—As shown in
Step 2—As shown in
Step 3—The positioned particles in the pockets 46 are monitored as a function of time, using the detection method appropriate, for the changes that occur upon positive (or negative) interaction between the particle-based and the positioning-fluid entrained species as described in Step 2. Changes in the detected signal for each particle are monitored as the analyte is flowed over the particle in the particle positioning fluid stream. Time-dependent interrogation in the chamber can provide additional real-time kinetic and dynamic information about the interactions.
Step 4—As shown in
The system of the present invention has many advantages. It can operate on a micro scale, thereby needing only very small sample volumes. It provides rapid, sensitive and inexpensive analysis of particles, allowing multiple particles to be interrogated simultaneously without interference from other particles. In addition, it is possible to interrogate a single particle at one time, and thus the present invention is not limited to analyzing multiple particles simultaneously.
Although the present invention has been described in considerable detail with reference to certain preferred versions thereof, other versions are possible. For example, the system of the present invention has many uses in addition to analyzing particles. For example, it can be used for chemical synthesis using particles as a solid phase support in which the synthesis occurs. It is particularly useful for a synthesis involving a reaction that can be monitored in real time. The small volume of the analysis chamber minimizes the volume of reagent that is required, and the sequential steps of a reaction can be carried out in one or more analysis chambers, utilizing the positioning fluid conduit for providing different reagents. As an example, a serial four-chamber system can sequentially apply guanine, cytosine, thymine, and adenosine into the chambers for oligonucleotide synthesis. Similarly, the system can be used for sample preparation, pre-concentration, extraction and clean up. Discrimination for low-concentrations of target analytes and high background analyte concentrations can be achieved by passing a sample over capture particles positioned in the analysis chamber. Accordingly, the scope of the appended claims should be limited to the description of the preferred versions contained herein.
All documents cited herein are incorporated herein by reference in their entirety. All features disclosed in the specification, including the claims, abstracts, and drawings, and all the steps in any method or process disclosed, may be combined in any combination, except combinations where at least some of such features and/or steps are mutually exclusive. Each feature disclosed in the specification, including the claims, abstract, and drawings, can be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features.
Any element in a claim that does not explicitly state "means" for performing a specified function or "step" for performing a specified function, should not be interpreted as a "means" or "step" clause as specified in 35 U.S.C. � 112, unless to sustain the validity of the claim.
|Cited Patent
|Filing date
|Publication date
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|Union Carbide Corp
|Electro-osmotic cell
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|Electro Dynamics Inc
|Electro-hydraulic transducer
|Aug 15, 1974
|Dec 2, 1975
|Alza Corp
|Electroosmotic pump and fluid dispenser including same
|Oct 1, 1998
|Jul 11, 2000
|Sandia Corporation
|Electrokinetic micro-fluid mixer
|Mar 18, 1999
|Aug 21, 2001
|Sandia Corporation
|Electrokinetic high pressure hydraulic system
|May 22, 1998
|Jun 18, 2002
|Lynx Therapeutics, Inc.
|Planar arrays of microparticle-bound polynucleotides
|Sep 26, 2002
|May 15, 2003
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|Bead trapping device
|Apr 1, 2003
|Apr 15, 2004
|Fluidigm Corporation
|Microfluidic particle-analysis systems
|Aug 27, 1999
|Mar 9, 2000
|Febit Ferrarius Biotechnology Gmbh
|Method and measuring device for determining a plurality of analytes in a sample
|Nov 27, 2000
|May 31, 2001
|The Governors Of The University Of Alberta
|Apparatus and method for trapping bead based reagents within microfluidic analysis systems
|May 14, 2001
|Nov 15, 2001
|Pyrosequencing Ab
|Microfluidic devices
|Apr 1, 2003
|Oct 16, 2003
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|Microfluidic particle-analysis systems
|Brenner, S.; et al., "Gene Expression Analysis by Massively Parallel Signature Sequencing (MPSS) on Micro Bead Arrays" Nature Biotechnology (2000) 18, 630-634.
|Kitamori, T.; et al., "Integration of an Immunosorbent Assay System: Analysis of Secretory Human Immunoglobulin A onPolystyrene Beads in a Microchip" Anal. Chem. (2000), 72, 1144-1147.
|Citing Patent
|Filing date
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|Oct 1, 2009
|Jan 8, 2013
|Koninklijke Philips Electronic N.V.
|Microfluidic device
|Jan 21, 2009
|Nov 19, 2013
|Sandia Corporation
|Micropores and methods of making and using thereof
|Jan 21, 2009
|Aug 26, 2014
|Sandia Corporation
|Methods and devices for immobilization of single particles in a virtual channel in a hydrodynamic trap
|Dec 19, 2005
|Mar 29, 2007
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|Micro electro-kinetic pump having a nano porous membrane
|Jan 21, 2009
|Feb 3, 2011
|Perroud Thomas D
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|Jan 21, 2009
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|Microfluidic device
|U.S. Classification
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|International Classification
|Cooperative Classification
|European Classification
|Feb 12, 2003
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text:
Cuil, pronounced "cool," has officially launched.
All the kool kids are talking about it. The question is whether anyone will use it.
As a new search engine, Cuil is a longshot. It's no Google Killer.
Check out Cuil.com. Google needs the competition. But don't expect a revolutionary search experience. The results page looks very much like Guy Kawasaki's Alltop.com.
Cuil was created by former Google engineers Anna Patterson, Russell Power and Louis Monier, who picked up $33 million in venture capital to launch the search engine.
So how is Cuil different than Google? They're claiming bragging rights for search index size: 120 billion Web pages. While Patterson says that's 3X the size of Google's index, most people acknowledge that size doesn't matter.
As Google's official blog notes, many pages not indexed either point to similar content or would diminish the quality of its search results in some other way. T
Of course, Cuil can't use PageRank to organize results. So Cuil apparently assesses the actual content of a page.
Cuil's results are most similar to universal search, displaying photos horizontally across the page. Sidebars can be clicked on to learn more about related topics.
In a nod to privacy, Cuil promises not to retain users' search histories or surfing patterns.
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text:
Published 23 April 2012 | Adelaide Writers' Week
Raising the Dead: History and FictionBooks and Authors
- Highlight
- watch now
This is a great panel from Adelaide Writers' Week with four outstanding writers who make meticulous use of historical archives to write fictional novels.
The writers are Javier Cercas, Jenny Erpenbeck, Kate Grenville and Ron Rash. Journalist David Marr moderates the discussion and opens with the question..."why, as writers, do you raise the dead?
Cercas explanation is..."You cant explain the present without ...the past", but he does go on to make this great statement..."truth is not always convincing!"; Marr comments that maybe the role of fiction is to make the truth convincing.
The discussion ranges over how each puts together and writes about the relationship between past and present; addressing the gap between the values of the present reader and the values of the characters of a particular period ; but they all resist labelling their works historical fiction.
Ron Rash is a writer from the American South. His works include the short story collection Burning Bright and the novel One Foot in Eden. He lives in North Carolina and he is Professor in Appalachian Cultural Studies at Western Carolina University.
Javier Cercas is a best selling Spanish novelist whose works include the experimental work about the Spanish Civil War, Soldiers of Salamis; Speed of Light about a Spanish novelist and Vietnam vet set in the US and Barcelona and his most recent work is the Anatomy of a Moment a non fiction work about the failed 1981 Spanish coup detat.
Jenny Erpenbeck has worked in opera and musical productions (she makes reference to being overlooked as an opera director for the Adelaide Festival!) but is also one of Germanys rising literary stars. Her books include The Old Child and the short story collection, The Book of Words. Her most recent work is the novel Visitation.
Kate Grenville is one of Australias finest writers with works including The Secret River, The Lieutenant and Sarah Thornhill. Other books include the Idea of Perfection which won the Orange prize for Fiction; Lilians Story; Dark Places and Joan Makes History.
Comments (5)
Strathpine Cookie :
19 Jul 2012 7:42:42pm
I saw the last of this today and didn't get the name of what was read prior to Ron Rash's reading from One Foot in Eden. Can someone please help?
Russell :
15 May 2012 6:27:34pm
Grenville is a positive pain in the posterior, her Secret River might have been a "rattling good story" but hardly great literature, trouble with writers is that they are often dull talkers but I suppose I'll have to put up with Grenville if I want to listen to the talk, David Marr should be make the sufferance worth while.
Susan Hose :
08 May 2012 12:07:53pm
This was fascinating to watch, especially since I am wanting to get my own work published one day. After watching this, I looked at my own work, (which is based in the distant future), and can see how issues of the past and present can still be present and still so relevant. Thank you all very much.
Peadar :
07 May 2012 11:52:27am
Great Panel;excellent moderation who all listened
'raising the dead' panel :
06 May 2012 2:22:51pm
fascinating and insightful discussion about the role of the past in fiction writing. Have read All of Grenville's novels, but none of the others. Their read excerpts led me to search for their novels. Thankyou.
p.s. David Marr's chairmanship was sensitive and witty.


text:
Executive Pastor at Proclaimers Norwich and Centre Manager of The Space
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Interested in a bargain?! We're buying new lecterns (pic) normally £350 each but have 12 for £150 - DM me!
@tomrawls inspiring the peopl at our BIG night of prayer
The cheese and crackers festival begins...
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Early soundcheck before we play the game live on the big screen!
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Making chairs while watching football!
Now that's a lot of chairs!
@tomrawls interviewing Dr @philtemple as part of our #healthcheck series - how to know if ur healthy
Beautiful wensum park needs some litter picking and bins. Sounds like a project for @lovenorwich
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Clinical-Style Validation, Regulation, and Quality Control...
Loss of Lives in Clinical Trials...
Capitol Hill Critics Second-Guess Agencies Using...
Co-Sourcing Improves Pharmaceutical Laboratory Productivity
Novozymes set up a biopharmaceutical ingredients business to develop and manufacture animal-free recombinant ingredients to support production. The initial focus will be the launch of a range of ingredients developed specifically for industrial-scale mammalian cell culture.
"We are very excited by the launch of this new business and what it represents to the wider biopharmaceutical industry", says Peter Rosholm, vp for the iIngredients business area. "We have combined Novozymes expertise in large-scale protein production and supply with a strong science base to provide a range of products specifically developed for biopharmaceutical production.
"In addition to addressing the need to produce ever-safer biopharmaceuticals, our cell culture range has a secure supply network to deliver ingredients that allow for process optimization whilst reducing customers regulatory and lot testing burdens."
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MDMA (commonly known as the empathogen "ecstasy") is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.Do you agree that ecstasy should be studied for its potential therapeutic benefits?
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Free Agents are players that would like to join an IM Sports team but don't have enough players to form their own team. To sign up as a Free Agent, you must follow the steps below.
Activate your RSF Membership
You need an RSF membership to play IM Sports. As a result of students voting to pass the Wellness Referendum in April 2015, all current and registered Fall semester Cal students are eligible for Fall IM Sports leagues but must first activate their membership prior to registering for IM Sports leagues. Your registration may be activated via the button below. Your status with the Registrar's Office must be current.
Non-students must purchase a membership to be eligible for IM Sports.
Pay the Free Agent Fees
Beginning January 25, if you've chosen to sign up as a Free Agent, you'll need to pay the $25 Free Agent fee at the Rec Sports Online Store. Once in the store, find the sport, day, and time that best fits your interest and schedule.
After paying the Free Agent fee, IM Sports will add you to a Free Agent team roster in Sportifik within a few days. You do not need to sign up as a Free Agent in Sportifik. Paying the fees will get you invited to the Free Agent team within a few days.
Set up an Account in Sportifik: The IM Sports Online Portal
Use the Sportifik system to create an account after you have paid the team fee. Sportifik is where you can track your new team's schedule and standings. You can also interact with your team via the team message board. A few days after paying the Free Agent fee your team info will appear on the site after logging in.
You do not have to sign up as a Free Agent in Sportifik – IM Sports will invite you to join the FREE AGENTS team a few days after paying the fee at the Rec Sports Online Store.
Important Note on Multi-team Enrollment
The Free Agent program is designed to accommodate individuals who are not already on a roster in the same sport. The fee covers placement on an IM Sports team roster and is non-refundable.
If a Free Agent later finds a different team or otherwise opts not to participate with the Free Agent team, no refund is issued. Sportifik blocks multi-team enrollment within the same sport – do not register as a Free Agent in the same sport if you are already on a roster.
Additionally, the Free Agent program is not intended to accommodate teams that are waitlisted or otherwise did not make it into the division of choice. Participants who abuse the Free Agent program will be banned from participation.
Step-by-Step Instructions
- Activate your Cal Rec Club membership to sign up for IM Sports.
- Create an account at Sportifik
- Review Free Agent schedules at Sportifik
- Search for teams named "FREE AGENTS" within the schedules. These are the only teams accepting Free Agents.
- Do not create a team in Sportifik unless you wish to create a full team of your own.
- Once you've decided on a sport, day, and time (not all sports have Free Agent teams), visit the Cal Rec Sports Online Store and pay the Free Agent fee for the sport of your choice.
- The Free Agent fee is $25 per Free Agent per team.
- Items in the store are clearly labeled as Free Agent items: IM FREE AGENT + (SPORT) + (GENDER). If an item does not have a gender, it is open to all.
- Once you pay for your Free Agent spot, we will invite you to join the FREE AGENTS team within a few days.
- You do not have to sign up as a Free Agent in Sportifik. We will invite you to join the Free Agent team you have paid for.
Captains: Finding Free Agents For Your Team
- Login to Sportifik
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Most people who attend Owens live off campus and commute to school. There are a lot of activities available to participate in but a lot of the students are working full time, raising families or starting families and have other commitments to attend to. Most students come to class and go home. The student body is very diverse, people from the greater Toledo area all come here for good education. I think going to college is about learning and accelerating your career, some students only care about having fun and that's where people go wrong, they care all about the wrong things. I have a few friends in my classes but outside of class I don't interact with anyone from the college.
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Automated Identification System
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Savage killing, sex at heart of Jodi Arias murder trial
There are 687 comments on the KTVK Phoenix story from Jan 8, 2013, titled Savage killing, sex at heart of Jodi Arias murder trial. In it, KTVK Phoenix reports that:
A nude Jodi Arias on Travis Alexander's bed. A naked Alexander in the shower. Then minutes later, an image of Alexander stabbed and slashed nearly 30 times in the heart, back, hands and torso, shot in the head, his throat slit from ear to ear.
Join the discussion below, or Read more at KTVK Phoenix.
#232 Mar 30, 2013
p.s. Speaking of behinds, what is her obsession with describing sessions of anal sex with him? Is that to try to further put out the pedophilia defense? Even if his door did swing that way, that is not a defense for her brutally killing him. I could see her steering away from him and maybe trying to get him to seek help if that were real. Nothing was found to substantiate her claims. I saw some testimony where she claimed that he rubbed against her and ejaculated on her back but that didn't make her angry. None of her stories make any sense. I don't think she has told the truth yet. There had to be something that made her snap. I also read (no I don't have a link) that she was ambidextrous and that might have been how she was able to do all the damage she did and clean up, etc.
I do think she thought she was smarter than others and could talk/flirt her way out of this situation. I don't buy the low self-esteem, PTSD and dissociative amnesia b.s. I think under the right conditions she could remember what she did. Unfortunately, there are laws against questioning a person in this manner. She gets all the rights. I saw the picture of his body stuffed in the small shower with all the wounds. She even went for his face and neck. She is a pit bull.
#233 Mar 30, 2013
Tex, you're right, we'll probably never know the truth. It's hard to get inside the mind of someone like her, but my reasonable mind keeps saying that there's just no justification for the death - particularly the brutality of it - that he suffered.
If we were all allowed to capitalize on and be excused for every injustice (perceived or real) against us in life, and slay our ways through it, we'd be extinct. Everyone's had problems and difficulties, but it's your own responsibility to address them. You can't just act on everything and then cry "mistreated and mentally off". We've swung so far to that in this society now that it's become a mantra.
If we don't stop the excuses, lack of accountability and responsibility, and start enforcing consequences for actions, we're sunk. We've set a precedent for excused violent and self centered behavior, and I think it starts with lazy parenting. It's easier to look the other way than address the reality of what our kids are doing.
#234 Mar 30, 2013
What sticks in my mind is that she a liar. Perhaps she has been able to lie her way out of things in the past and thought she could do so again.
Why do people feel sorry for someone who shot a person and when that person became incapacitated, she chose to stab him and slit his throat. I saw pics of the stab wounds to his head, neck and his slit throat. I'm not sure there is any sentence that would be appropriate for this. Maybe time in a jail cell with Manson? I believe if she got out, she would be a threat again. I am relieved that they are denying everything requested. At the very least, she needs to be behind bars the rest of her days.
United States
#235 Mar 30, 2013
You have an open mind, which many posters lack. Also give you credit for doing your research. So apparently you are a female (bangs).
Not bragging, but I called this one the first few pages of the main forum on this one. Even mentioned these extreme BDSM scenarios and that it can all result in tragedy and mayhem. Most women do like some Mild B&D, mosts are subs. TA was an Alpha Stud and was uping the ante with JA. Who knows how he got started into the B&D scene. Strange, when I was in my early 20's some very nice and proper GF's asked to Dom them. I had never even thought about it previously. At times it's just natural chemistry, especiall when a guy is holding strong and has the stamina.
United States
#236 Mar 30, 2013
Tell us something we do not already know, as to prosecutorial considerations. I do agree with financial considerations not being part of the equation, in true DP cases. In Cal, just last year, there was a ballot measure to abolish the DP since it costs too much (failed)
JA was overcharged, DP cases must be reserved for a selected few...she does not fit that profile...I'm a proponet of the DP, but cases like this one play into the hands of the abolishment movement.
Do you have one of those Konji tatts? Think about a different AKA.
#237 Mar 30, 2013
Since you have more knowledge of the 50 Shades of Grey Darker kind of scenes, what do you postulate happened that she was taking pictures of him in the shower one minute and 2 minutes later he was dead with his throat slit, 29 stab wounds to the back and neck areas, and a gunshot wound to his face? All of this done by one 125 Latina female? He looked pretty stocky in pics. It seemed like he could have fought her unless he was frozen with fear or she incapacitated him with the gunshot wound or blinded him in some way. What are your thoughts?
Some say he was just toying with her emotions and that you should never play cat and house with a potentially psychotic person, must less a sociopath/borderline person.
Sorry, but Samuels comes across as totally incompetent. Seems like maybe someone got to him and he flubbed things on purpose. Not sure about LaViollette. Looking at her size, I'd say she was a victim of abuse herself and therefore is going to see abuse whether it was there or not. Just some off the cuff opinions.
#238 Mar 30, 2013
Maybe Jodi was reading 9 1/2 Weeks. Travis was no Mickey Rourke and she was no Kim Basinger.
I haven't read the book, but it was supposed to be taken from a real woman's journal. I believe the woman was an English professor and got whisked off into the world of dom/sub. It was fun and exciting at first. Then, at some point, he tried to bring another party into the equation and she freaked. However, she left him instead of killing him. He let her know that he had done that many many times before with many other women. She asked him how he knew she would respond in the way she did and he said he saw himself in her.
I find myself just as annoyed with Jodi as Travis was and I don't even know her. I can't find a way to feel any compassion for her. She drove to him. She kept herself in that situation. She knew he wasn't seriously considering her for anything except sex. Her descriptions of sex don't even make sense sometimes. She would make a very bad writer of sexual material. The excerpt I read from 50 Shades of Grey was terrible writing, but Jodi's is even worse!
Since: Mar 13
Location hidden
#239 Mar 30, 2013
Tex here is a link that speaks to the clothing Arias wears.
I think it is a mighty big jump to assume LaViolette may have been abused herself based on her "size." It could be due to medication or physical issue (thyroid problems).
#240 Mar 30, 2013
I guess I should have further explained that I have worked with quite a few women in support groups that were LaViolette's size. I don't know of any that size that were not victims of either sexual abuse or physical abuse or both. It's just a comment, not a judgment or statement of fact.
Since: Jan 13
Bundoora, Australia
#241 Mar 30, 2013
lets stick to this murder and not go off track
Since: Jan 13
Bundoora, Australia
#242 Mar 30, 2013
Micky Rourke isn't even Micky Rourke any more.
Since: Jan 13
Bundoora, Australia
#243 Mar 30, 2013
La, I bought into your BDSM stuff at the beginning, then I wondered if there was some truth to the intruder story since that neck wound is pretty astounding. nice clean cut like nothing I can get done in my kitchen.
However, I can get ALV testimony. When you marry that with Chris and Sky Hughes and how their emails have been such a point of contention. JM didn't want them admitted. Sky said she didn't want her sister to date TA. It was Sky who introduced Jodi to Ryan. I don't know what to think of the Hughes except they made up their mind and didn't wait for the police investigation and the testimony at court and for the jury to decide. If you go to youtube and watch day 10 part 3 and see chris hughes being crossed by nurmi. Chris has to read a three page response to Travis email. He wrote it. He should be familiar with it but he takes ages to read it. For a smart man it is too long. He then asks what part they want to focus on. Nurmi said read the whole thing. He is not giving him any clues. Martinez objects and during the side bar you get to see chris reading his email and then he starts nodding his head like he has worked out what he will say. "yes, I'll spin it this way". Except Nurmi hardly lets him speak and he looks frustrated. If I were inclined to flights of fantasy I might wonder if the Ninja story is true. How come those letters that travis wrote were only photocopies? What happened to the originals? How come how travis died is suspiciously like blood atonement? How come Jodi allegedly stole the gun but Travis was really killed by a knife? How was she able to achieve all that in 96 seconds? Why does Jodi say in her police interview "it is better if people think I am the only one involved" Flores tries to get her to cough up another name.
Since: Jan 13
Bundoora, Australia
#245 Mar 30, 2013
If his door swung that way with an adult partner it doesn't matter. If his door swung that way with a child then it does matter. There is the layering of the recorded phone coversation where she is evidently leading him. As a man I could not be lead into that particular fantasy. EVER. I am not interested in anyone I can't have a reasonable conversation with. What do you talk about with 12 year olds? Barbie and the Crystal Palace? The Hughes emails are particularly telling. Go to Day 10 part 3 of the trial with Chris being crossed. JM didn't want the emails admitted. They seem to be a confession of Travis for his pedophilia.
Since: Jan 13
Bundoora, Australia
#246 Mar 30, 2013
I am not sure about family but certainly the Mesa Mormon community has a lot of clout simply because of their numbers there.
I think it was a foolish move. I believed the St Travis version before trial. Now I think he was a pedophile. I am not sure all the truth will come out but this trial has just added another layer to the Jeffs controversy. And I am going to make sure the next lot of Mormon missionaries that knock on my door know how I feel about them because of this case.
Since: Jan 13
Bundoora, Australia
#247 Mar 30, 2013
What about the Hughes email correspondence?
London, Canada
#248 Mar 30, 2013
all religion is messed up
United States
#249 Mar 30, 2013
Buddhist/Confuscious/Lao Tsu quotes of day may enlighten you. I like to start my day with them. Just Google them. We all need to seek humility and the Eastern Winds truly illustrate this.
Religous insitutions (Catholic, Jewish, Protestant) have contribted much to our N American civilization ... just look at the most advanced Universities and Medical Institions.. I was just in DT LA today and drove by Cedars Sanai and St Vincents and
reflected on the contributions of the big three.
There is the good and the not so good to Religions as we have known them.
JA & TA were seeking a higher path, but carnal desires overuled the prudent Mormon teachings.
United States
#250 Mar 30, 2013
You must be aware of Samuel Clements (Mark Twain). Here' one of his quotes:
Go to Heaven if you want good weather.
Go to Hell if you want good companionship.
#251 Mar 30, 2013
I don't know yet. I haven't seen the entire email to put what was referred to into context or heard any other testimony about it, and I'm not sure ALV isn't in someone's pocket or very biased. She seems to have taken what Jodi said and weaved it beautifully into her testimony, and that makes me suspicious. I respect her field and it's very necessary but so many professionals tend to read our studies into every situation. Cops become cynical when dealing with criminals daily, too. There is abuse, and then there is name calling. I'm not sure I can put them on the same level in my mind and justify violence in every case of a man not being a gentleman to a lady.
The Hughes, like many of Travis' friends, make me cringe with their self righteous attitudes and superior demeanors. I detest arrogance and sense so much from all this crowd (including Travis and Jodi). I believe they'll be back into this trial at some point so I'm going to wait for their spin on it.
#252 Mar 31, 2013
I went back and tried to watch the part where Chris was re-reading the e-mail. Yes, it took forever. It seemed like this was a stalling tactic. My patience wore out and I went to watch the 48 Hours interviews that included Chris and his wife, Det. Flores and others. It's funny that Jodi first said she wasn't there at all, but then when the photos showed up, she admitted she was there but that she had nothing to do with his murder. She even made the challenge that no jury would ever convict her. She's been watching too much "Big Love" with Roman and the polygamists. In the fundamentalist compounds, they do live by different rules/laws. As far as I know, she is not a Fundamentalist Mormon and wasn't interested in the religion until she was trying to reel in a big fish, Travis Alexander.
Interestingly, enough, when asked about her childhood, she said it was pretty much perfect. Did I hear that right? Somewhere I saw where Travis Alexander's was left than perfect with two parents addicted to crystal meth and that the kids were rescued by grandparents. I don't know at what age he was rescued.
I wasn't all that interested in this case. Then you get sucked into the vortex. She now has admitted she did all of it alone. There is no other DNA at the scene, taking away the intruder story possibility.
I also watched a reenactment where it was believed that the fatal stab to the heart was first and that he was then dragged into the bedroom where his throat was slit and that he was shot in the bathroom and stuffed into the shower. Others think he was shot first which I tend to believe. I would think she would have slit his throat next so he couldn't scream for help and then made the final blow to the heart , but I could be wrong. As far as dragging him around, how was she able to do so? There must have been a lot of adrenaline pumping for her to do all that in 96 Seconds.
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