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1992: Happy anniversaries in Washington, D.C.
Keith Bundy breaks into a big, happy laugh, "I love the outdoor space. And I love the kitchen," he says. "I love to cook, and meals are so important to the family core, just being together."
The home Bundy moved into with his wife, Pam, and their three children in 1992 is one of 10 built in Washington, D.C., when the Carter Work Project brought the former president and first lady back to town.
Bundy remembers lots of news coverage and being quite happily surprised with the entire process. "I never thought that it would happen so fast," he says. "After 6 months labor, we were in the house." He lets out an amazed chuckle, remembering what felt like a whirlwind. "Just before the build, we came out to the site where the home was to be built, and it was just a concrete slab. And we finished the build before that week was out."
As years have passed, two of the Bundy children have moved out. The third, a son with a physical disability, lives with Keith and Pam, who has health issues, as well. Their conditions give Keith one more item to add to the list of things he loves about his house: no stairs. "I love that everything is one level, especially as I see my wife struggling," he says. "She's been struggling the last few years with balance and walking, so the one level is terrific."
Having a stable, affordable place they love to call their own for the last 21 years has helped the Bundy family focus on more important things like health — and each other. The day they moved in, June 18, is twice as special for them. This year, Bundy explains, "My wife and I celebrated our 26th wedding anniversary on the anniversary of moving into the house."
ALSO IN 1992: In addition to the 10 homes completed in D.C., 10 row houses were rehabbed in the Sandtown area of Baltimore.


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subset: commoncrawl


text:
Honolulu, HI - "el pollo loco" search results
About El Pollo Loco
El Pollo Loco, Spanish for, "The Crazy Chicken," is a Mexican chain restaurant. Originally starting in Mexico as a roadside food stand, this small chicken chain made its debut in the United States in 1980. Hence their name, "The Crazy Chicken's" speciality is their juicy and tender, flame grilled Mexican chicken. This fast food corporation is also highly involved in charity fundraising and community events. They have developed their own, El Pollo Loco Charity, a nonprofit 501(c) charity program which was both designed, implemented and is now run by volunteers from the company. They are committed to serving the community through both their charity efforts and by using only fresh, authentic ingredients. Every franchise uses only the best quality avocados, peppers and tomatoes, chopped and diced daily, and serves up only the freshest chickens availble.�
Loco Boutique
150 Kaiulani Ave, Honolulu, HI 96815(808) 922-7160 Write a review
Loco Boyz Surf School
744 Kapahulu Ave, Honolulu, HI 96816(808) 739-5588 Write a review
Loco Boutique
2211 Kuhio Ave, Honolulu, HI 96815(808) 926-7131 Write a review
Loco Trendz
2255 Kuhio Ave Ste 779, Honolulu, HI 96815(808) 924-5351 Write a review
Loco Wind
140 Liliuokalani Ave Ste 201, Honolulu, HI 96815(808) 926-4445 Write a review
Loco Moco Drive Inn
140 Liliuokalani Ave Ste 201, Honolulu, HI 96815(808) 625-8778 Write a review
Loco Moco Drive Inn Waipio
94-800 Ukee St Ste 301, Waipahu, HI 96797(808) 676-2888 Write a review
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Kunia Wal Mart Ctr, Waipahu, HI 96797(808) 688-2868 Write a review
Loco Boutique
Kunia Wal Mart Ctr, Waipahu, HI 96797(808) 943-2445 Write a review
Eggs 'n Things
343 Saratoga Rd , Honolulu, HI 96815(808) 538-3447
3 Dishes Yelpers Love at Honolulu's Best Brunch...Write a review
More on El Pollo Loco
Although known in particular for their delicious and authentic style Mexican chicken, El Pollo Loco offers a varied Mexican menu. They frequently offer promotional deals and incorporate new items into their menu. Many family chicken meals are available to select from as well as individual sized chicken meals. Their menu also includes a value menu and kids combo menu. For those with special dietary or health concerns, El Pollo Loco has a "healthier for you" section as well as a section for items under 500 calories. For those who are wheat gluten intolerant, wheat gluten free corn tacos and tortillas are available. Traditional Mexican sides, such as pinto beans, Mexican rice, flame grilled corn and sweet corn caked are also offered. Mexican dessert favorites such as churros and flan are available for dessert. Nutritional information for all of the menu items can be downloaded online from their official website or can be provided upon request in any local franchise.


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Interest Level
Grades PreK - 1
Reading Level
Grade level Equivalent: 3.5
Lexile® Measure: 600L
Guided Reading: K
Type of Book: Read-Aloud Book
- Classics
- Dolls, Toys, Puppets
- Love
- Determination and Perseverance
- Friends and Friendship
About This Book
Don Freeman's classic character, Corduroy, continues to stir the hearts and imagination of children and adults around the world. Published thirty years ago, this children's book, in which a bear leaves the safety of his department store shelf in order to do almost anything to be adopted by a little girl, is one of the most endearing and enduring in existence. Instilling the theme of hope and compassion, there's no doubt that this beloved picture book will continue to be a favorite for each new generation waiting to have their own "Corduroy" experience.
Born in San Diego, California, Freeman moved to New York City in the 1940's to study art while making a living as a jazz trumpeter. He died in 1978, leaving his beloved audience with a treasury of classic children's stories. Corduroy is a story that all children can relate to.
Items Added to Cart:
1 Paperback
2 Hardcover
1 Teacher's Edition
$48.99 Subtotal


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My Mother's Records
As I've noted before, I grew up in a Sinatra-loving household, both my parents worshipped the ground he walked on and to this day I can't see a Capitol Records label or one of those Reprise Records labels with Sinatra's photo on it without picturing it on the turntable of our old mono Bush record player.
My mother's favourite Frank Sinatra record has always been the slinky "Witchcraft" while I'm of the rather cliched and uninteresting opinion that "I've Got You Under My Skin" is his the best thing he ever did. But just because it's the conventional view doesn't mean it's wrong, I think it's one of the greatest recordings of 20th century popular music.
So you'd think I wouldn't be inclined to like The Four Seasons smooth, easy listening version of the song from 1966 but it's a record that my mother played to death when I was a kid which gives me a certain rose-tinted view of it and how do you separate pop records from your memories of them? Impossible I think (there's a post idea right there). It lacks the passion and hunger of Sinatra's version and takes a rather more dreamy approach but I still think it's very pretty with a sweet arrangement full of strings and bells, and I love the big drum break after the pause at the end.
Download: I've Got You Under My Skin – The Four Seasons (mp3)


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Ebenezer Pentecostal Church Assemblies of God, will be having our 2nd annual shoe box packing party. Bring your child\'s shoe box items and join us; as we celebrate the \"Power of a simple gift\".


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Heather Shirley Venable posted this tip on the Bargain Mom wall -- Join us for our GRAND OPENING tomorrow & Saturday! There will be door prizes & refreshments available! We will be open 10am-6pm both days. Please help us spread the word. We would love to have a great turn out! The store is filled with great, unique things you won't find in any other stores! When you shop with us, you are supporting over 25 local crafters.
You can see some of the things we have to offer on our FB page too: https://www.facebook.com/


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In 1992 millions of people in cinemas around the world laughed their hearts out watching Whoopi Goldberg and her nun sisters. In the musical version we again meet Deloris the nightclub singer, who is hiding in the convent from gangsters and making the convent walls shake with her nuns' choir. Their antics have audiences at the Stage Metronom Theatre at the CentrO Oberhausen in stitches.
What could be more whacky than a tale about a nightclub singer having to hide from mobsters in a convent? In 1992 SISTER ACT brought a richly comic story of two totally different worlds on a constant collision course to cinemas and became a massive worldwide hit.
The stage version is just as brilliant as the film. When Deloris van Cartier, a talented nightclub singer, escapes from a group of gangsters and ends up in a strict Catholic convent with nuns whose singing is not what it might be, everyone's in for a shock. But the audience of 1,800 at the Stage Meteronom Theatre at the CentrO Oberhausen is in for a treat. SISTER ACT is an unforgettable experience full of energy, divine singing and toe-tapping tunes. As Whoopi Goldberg, who co-produced the musical, says: "You'll have a fabulous time! There's no deeper meaning, no hidden messages – just go to the theatre and you'll have a fantastic evening. You don't have to be a genius to see this show. Just come along and have fun!"
Lisage siia oma lemmikud. Kinnitage, liigitage, jagage ja printige oma valik ning plaanige nii terve oma Saksamaa-reis.
0 Lemmikud on välja valitud
Kaks kasulikku klahvikombinatsiooni brauseris suumimiseks:
Suurendamine: +
Vähendamine: +
Täpsemat teavet saate, kui klõpsate brauseri pakkuja ikoonile:


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BEGIN:VCALENDAR PRODID:Godengo, Inc VERSION:2.0 METHOD:PUBLISH X-WR-CALNAME:Calendar X-WR-CALDESC:This is a calendar exported from the Rivista calendaring syste m X-WR-TIMEZONE:US/Pacific BEGIN:VEVENT UID:firstname.lastname@example.com DTSTAMP:20161021T231039Z CATEGORIES:MUSIC DESCRIPTION:
\n Jim Brickman has revolutionized the sound of solo piano with his pop-style instrumentals and star-studded vocal collaborati ons.\n
\n With 6 Gold/Platinum albums and multiple PBS Spec ials to his credit\, Jim is currently Billboard&rsquo\;s most charted Adul t Contemporary Male Artist. Being that he normally performs in much bigger venues\, we&rsquo\;re extremely proud to be offering a truly rare matinee &rsquo\; performance by this masterful musician/songwriter.\n
\n Sin
ce the release of his debut album No Words in 1994\, Jim Brickman&rsquo\;s
romantic piano sound has made him the best-selling solo piano artist of o
ur time. Brickman will bring one of the most entertaining and romantic con
cert events of the year to cities across the country in 2013. An Evening w
ith Jim Brickman\, brings Brickman&rsquo\;s signature solo piano melodies
and hit songs to life\, plus audiences will experience brand new music fro
m his hit CD\, Believe.
\n Brickman'\;s best-known compos itions include the chart-toppers &ldquo\;Valentine\,&rdquo\; &ldquo\;The G ift\,&rdquo\; &ldquo\;Love of My Life\,&rdquo\; &ldquo\;Simple Things&rdqu o\; and &ldquo\;Peace.&rdquo\; He&rsquo\;s established a reputation for hi s collaborations with many gifted musical artists: Lady Antebellum\, Marti na McBride\, Kenny Loggins\, Michael W. Smith\, Carly Simon\, Herb Alpert\ , Collin Raye\, Michael Bolton\, Donny Osmond\, and Olivia Newton-John.


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I had gastric bypass 4 years ago and lost 200 pounds. I have extra skin on my arms I would like removed and I also would like a Breast lift or Breast implants. Last year, I had emergency bowel obstruction surgery and got a large hernia as a result. I want to get all three procedures done at one time: Arm lift, Breast lift, and hernia repair. Is that possible?
Arm Lift, Breast Lift and Hernia Repair All at Once?
Doctor Answers 13
Combining cosmetic procedures with hernia repair can be done safely depending on the patients health..
Combining procedures can often be done safely depending on the complexity of the procedures, the health of the patient and the risk factors for complications. Each patient must have a thorough evaluation prior to having longer operations that have a large elective or cosmetic component. It is best to have your surgeons and your medical doctor review your specific situation and health issues to evaluate whether you are a good candidate.
Web reference: http://www.casas.md/breast-lift/
Combined Surgery?
Thank you for the question.
Combined surgery is very commonly and safely performed. Whether this can be done in your case should be based on careful consultation (history, physical examination, and consideration of risks/complications/operating room time).
Based solely on your description, the breast and arm surgery certainly can be done in one session (assuming you are medically cleared to proceed with surgery). The "large hernia" probably should be addressed first.
I hope this helps.
Web reference: http://www.poustiplasticsurgery.com/Procedures/procedure_armlift.htm
Multiple body procedures
I think that if you have a complicated hernia to repair, you may be better off fixing that first and then having the other procedures done at a later time.
Web reference: http://www.stevenwallachmd.com
You might also like...
Combination surgery is challenging to coordinate
No question- the arm and breast lift can be done safely together- this is a combination that makes a lot of sense. Your complex abdominal wall reconstruction should be done in a hospital setting by an experienced general surgeon. I'm a board certified General Surgeon as well as a plastic surgeon, and I would never consider doing these three operations on anyone. Its going to be tough to find two surgeons willing to work simultaneously to pull off these three big operations. Take care of your functional problem first- the hernia- and then think about doing the cosmetic procedures.
Web reference: http://www.seattleface.com/html/after_weight_loss.php
Do breast lift and arm lift together, but not hernia repair.
The breast lift and the arm lift should be done together. In massive weight loss patients, those incisions have to be coordinated. But the hernia repair can be a big deal, and I would do that separately.
Arm Lift, Breast lift, and hernia repair in one surgery
It is really NOT an issue of CAN they all be done at the same time. It IS an issue of SHOULD these three operations be done together generally and then, in you.
As Plastic surgeons were are Surgical Super-Specialists. The vast majority of Plastic surgeons completed and were Board Certified in ANOTHER surgical field BEFORE training in Plastic Surgery -- with the vast majority having trained fully in General Surgery.
As a former General Surgeon, I can tell you that Hernias do not get the "respect" they should be getting. Hernias still kill thousands of people every year and their management can be quite complicated. You often read about hernias recurring despite repairs by good surgeons and of intestine twisting and dying inside hernias.
I would STRONGLY advise you to get your hernia repaired first BEFORE embarking on other repairs. If you wanted to combine the hernia repair with a Tummy Tuck repair (which may be simple or require a COMPONENT SEPARATION TECHNIQUE) I would have no problem with that.
I would save the Breast Lift and Arm lift for a later time once the hernia has healed and does not pose a threat.
Good Luck.
Consult with your surgeon.
Thank you for your question.
It techinically is possible. However, it is important to weigh the benefits vs. the risks. Your hernia repair will definitely take prescedent. It is important to take care of the necessities first. I would schedule a consultation with a board certified plastic surgeon. He/she should consult with your primary doctor and general surgeon and they will figure out what is the best option for you. It may be best to stage the surgeries. It all depends on your medical condition and other factors.
Best regards,
Dr. Speron
Multiple procedures
The fact that you describe your hernia as large and occured after a complicated bowel obstruction strongly suggests that your first priority should be the hernia repair. Discuss this thoroughly with your general surgeon who will do this repair and may possibly use a mesh. The potential problem is that the general surgeon will likely have to do an extensive dissection to free all of the scarred tissues. Also, if the hernia is so large the compression resulting from a "tight" closure can potentially impair your post op lung function and you need to concentrate on this. The length of time for these multiple procedures and the signifantly increased risks I feel negate considering these together. One consideration would be to do the hernia repair and IF everything went extremely smoothly (better than expected) and if the plastic surgeon is willing to "hang around", then the procedure could possibly by done. But this seems unlikely. Erring on the part of safety for the patient is hardly an error in the end.
Arm Lift, Breast lift and Hernia Repair at same time
I have done many body contouring procedures. I have done these procedures at the same time. However, I had to use a plastic surgeon as an assistant in order to speed up the process.
Make sure your surgeon is board certified and that your procedures are being performed at an accredited facility.
Multiple procedures common with Massive weight loss
It is very common to have multiple proceudres done at the same time in massive weight loss patients. However, the decision is based on the risks vs the benefits of doing multiple surgery, vs individual procedures. You also would need to have an assessment from your medical doctor.
As a plastic surgeon we always defer to the primary surgeon and his procedure. If they fel that their procedure should be done by itself we respect that opinion. If they approve then we discuss the positives and negatives with the patient
These answers are for educational purposes and should not be relied upon as a substitute for medical advice you may receive from your physician. If you have a medical emergency, please call 911. These answers do not constitute or initiate a patient/doctor relationship.


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Students from Miller Education Center West's MIKE Program (Multicultural Integrated Kidney Education) dedicated a mural they painted in a celebration on Wednesday, May 14. The MIKE Program teaches that health is a positive balance of social, emotional, and physical well-being. The mural sums up 6 months of hands-on health education.
John Solano
Reanna Smithey
Sarah Place
Makala Wolf
Haven Meyer
Jesus Cardoso Bobadilla
Erik Pintor Delgado
Rachael McBain
Samantha Rogers
Lauren Mapes
Gilberto Catalan
Mayra Bautista Gomez


text:
Europe can't seem to shake off the lingering effects of the Great Recession, but is that about to change? Recent data indicate the eurozone could be turning a corner. In the video below, markets analyst Mike Klesta dives into the data and discusses some ways to invest, including the Vanguard FTSE Europe (NYSEMKT: VGK ) as well as Dow stalwarts Coca-Cola (NYSE: KO ) or McDonald's (NYSE: MCD ) .
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Allynn Howe
We rely on our partner organizations, the Center for Responsive Politics and the National Institute on Money in State Politics, to uniquely identify individuals' campaign finance and lobbying activity. If data appears to be missing, it may be that they have not identified all influence activity for this individual. If the data is completely different than you expected, it may be that you're looking at a different person with the same name.
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Click here for campaign contributions with the name "Allynn Howe".
Campaign Finance$1,000 Given
Figures are based on itemized contributions reported to the Federal Election Commission and state agencies. Please note that:
- contributions under $200 are not reported, and so are not included in totals.
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- contributions are matched based on organization and recipient name reported within each election cycle. Contributions using an incorrect or non-standard version of the name may be missed.
- corporate name changes and mergers may cause figures to differ from those of the Center for Responsive Politics.
- organization totals include known subsidiaries of the organization.
For more information, please see our campaign finance methodology page. Lobbyist bundling data is described on our lobbyist bundling methodology page.
Top Politicians
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Lobbied with the Following FirmsHowe, Allynn
Most Frequently Disclosed Lobbying Issues
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Clients of Firms Allynn Howe Worked ForCouncil of Better Business Bureaus, City of Cerritos, CA, City of Waterloo, IA, Village of Matteson, IL, Midwest Affordable Housing Corp, City of Belzoni, MS, City of Fayette, MS, City of Laredo, TX, Dayton Philharmonic Orchestra, Jackson Medical Mall


text:
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No Love Lost Between Ann Coulter + World Net Gayly
Damn you, Ann Coulter. Damn you for making me fall in love with you for five seconds! After signing up to headline GOProud's Homocon in September, the incredibly rude Coulter found herself sparring with World Net Daily editor Joseph Farah, who dropped her for his own conference. (Coulter says she was never officially booked.) Coulter fired back calling Farah "swine," and then went on television to say more mean things!
Farah, who has a whole website to use as propaganda against Coulter, follows up: "Coulter called me a 'publicity whore' for my decision. But look who is on television talking about this – throwing mud, name-calling, smearing not only me but my entire staff. I will not engage in the kind of ad hominem attacks that have made Coulter so famous and that are making her even more of a media darling in this age of reckless anger and character assassination for the sake of entertainment. Every day, since we made this decision at WND, I thank God for giving me the clarity of mind and discernment to make the right choice."
What happens when neither the liberals nor the conservatives want to be on Team Coulter?
[Ed: We don't link to WND, but you can copy/paste the link if you really want to go. http://www.wnd.com/index.php?fa=PAGE.view&pageId=194229]
Eric Thor
The guy in the middle of the video with the fro, was a bit awkward.Aug 23, 2010 at 12:35 pm · @Reply ·
It's all about publicity and getting her name in print for Coulter. I would think this would be obvious by now. She would appear at the opening of an envelope on LOGO if asked; she doesn't believe half of what she says, she just says and does what will sell her as a personality. I would bet anything that she's a huge Madonna fan because her career arc follows Madonna's clsoely; she says and does increasingly outrageous things like Madonna did in the 80's & 90's, which culminated in the near-porno book "Sex" for Madonna. I don't know what Coulter's bullshit is going to culminate in but I'm assuming she's going to call for Obama's execution or something else that's not going to be possible to come back from as an encore. Then she'll fade away a rich, right-wing hero. I don't know if this makes her as bad or worse than the true believers; I tend to think better because while she does indeed cause our side damage occasionally, she's also using the right-wing for her own financial ends and playing them for a fool, which makes me smile a little inside.


text:
For the first time at Roosevelt High School the girls were on par with the boys in receiving special scholarship money.
A grass-roots committee that has been raising money to correct an inequity for young women at the Portland school reached its high mark with Wednesday's distribution of almost $50,000 to five seniors.
"These are amazing young women, they have worked so hard, and we are so proud of them," said Principal Deborah Peterson.
Berenise Delgado, Emily Walters, Anthea Vang, Zoraida Navarrete and Fakiya Omer received the scholarships at an awards ceremony in the school auditorium. They didn't know they were receiving the award and the amount they were getting.
"It means a lot. It means that all my hard work has paid off," said Walters, who will pursue a degree in radiology this fall. "I'm always striving to be better at school, and this proves that I am smart and I can achieve anything."
That's the idea behind the Theodore Roosevelt Women's Scholarship Association. It was founded to counter a scholarship fund set up only for Roosevelt's young men.
After a student was killed, the boy's family bequeathed money to the school to set up the Mock Scholarship, with the stipulation that the money go only to male students.
The school tried for years to change the endowment to include female students but didn't succeed, so the women's scholarship association formed with the task of raising money for them.
The group awarded its first grant in 1991. This is the first year it was able to award almost $50,000, the amount the young men get every year.
Part of the money came from Oregon State University, which matched the grant given to two of the seniors who will attend the university in the fall.
The rest of the money came from donations by businesses and private individuals, and from interest on the association's investments, said Charlotte Cook, a member of the women's scholarship association board.
"The money is necessary, but I think the encouragement and the faith we have in them is equally important," Cook said.
As for the Mock Scholarship, five young men were awarded $9,920 each: Deondrae Rhone, Rodrigo Perez Carrasco, Terrell Malley, Jeffrey Moua and Jordan Erickson.
This year was also special at Roosevelt for another reason: Vincent Spillman became the school's first Bill & Melinda Gates Foundation Scholar, which comes with a scholarship worth up to a half-million dollars.
-- Phillip Swarts; email@example.com


text:
Gemini woman is extraordinarily talkative. Her constant need to precise her views and ideas makes her an excellent communicator. Exploratory in nature, she likes to travel and find out new places and gain novel experiences. However, such people have an inclination to divert from their actual destination and therefore the new-found which means would be a lot of necessary to her than the initial one. Witty, charming and provocative, Gemini females are simple to approach and friendly.
A Gemini woman has an optimistic outlook in life. She tends to be terribly rational in her thoughts and ideas. whereas this tendency will result in someone being extremely smart, versatile and quick-minded, it may create her dispersed and mentally fatigued. A Gemini woman longs to be a baby at heart and can still do therefore all her life. She would love the exciting little adventures that she meets along her manner in life.
It solely in middle ages that a Gemini woman would begin acting mature and have a spotlight in life. A multi faceted individual, she has several friends and may be a totally different person in every of the social cluster she belongs to. She includes a wide selection of interests and goals still. A Gemini feminine is additionally a multi-tasker and may accomplish lots of things at an equivalent time. amendment is basically the foremost necessary a part of a Gemini woman, a requisite for her emotional well being.


text:
Latest Post:
Started By:
14 days ago.
by amos57usa
Letters To Ed :-)
"We've been duped by the Republicans. Kentucky Sen. and minority leader Mitch McConnell in a news conference one day after the election, stated that the single and sole goal of the GOP over the next two years is: to make Barack Obama a single term president. What an arrogant and obnoxious goal and statement. There agenda before the election was to get votes by promising that there first goals were jobs, the economy and health care reform. It seems their goal is to grab power, not to do things for the people that are in need of jobs and or unemployment pay, health care for those that don't have it, and the people losing their homes. This is the same party that wanted the auto plants and their suppliers to go out of business. We are just coming back from what they did to this country, and now we have them back to do the same things to our country again. What a mess the next two years is going to bring to this country. Talk about partisanship." Carl D. Leow Rogers Cit
Member Comments
Why is it when lefties, not only got draft deferments, but actually aided and abetted the enemy, they are brave, strong, peacenick, heroes, etc. but when conservatives get deferments, they are cowardly scum? Any of you non-commies out there notice that? Any non-commies out there? Here's a little something for the commies then: Baaaaaaa.
Two Presidential terms for Ted Nugent are in order. You know, just to separate the namby from the pamby, which, as we have already seen below, there's a 100% chance of today.
Obama should invite, as his guests, 20 million unemployed folks, the number-crunchers at Standard and Poor's, any living relatives of Marx and Engels, and a talented bugle player to perform a haunting rendition of Taps.
I'd rather carve a birch tree canoe with a spoon than listen to anything O' has to say. Picking out truths from any of his teletubby talks is like an impossible scavenger hunt, where the only thing hidden is truth.
Posted 1353 days ago.
Then there's this from the gOP:
"I am excited to have a patriot like Ted Nugent joining me in the House Chamber to hear from President Obama," Congressman Stockman said in a press release. "After the Address I'm sure Ted will have plenty to say." That's a good bet; apparently Stockman is encouraging reporters to interview Nugent after the speech. Nugent, of course, is best known for making this claim, which was investigated by the Secret Service:
"If Barack Obama becomes the president in November again, I will either be dead or in jail by this time next year," Nugent said, according to a video that the NRA posted on YouTube. "If you can't go home and get everybody in your lives to clean house in this vile, evil, America-hating administration, I don't even know what you're made out of."
So there's either going to be an assasination attempt or Draft-dodging Teddy is going to commit suicide, stay tuned.
Posted 1353 days ago.
After the Republican response to President Obama's SOTU, the Tea Party and Rand Paul is going to give a response. The introduction should be something like this: "Thank you Senator Rubio for the response of The Stupid Party (per Bobby Jindal). Now if you thought that was crazy, wait until you get a load of this one....."
Posted 1353 days ago.
Also, you need vocabulary assistance, as your tired little schoolyard names calling and taunts are not clever, as you seem to think, they are merely sophomoronic.
Posted 1354 days ago.
It's comical, pathetic, really, how you (shrill) have been WRONG about nearly every issue that comes down the pike. We all remember fondly, your misdiagnosis of the landslide election, and your unwavering support of the primary frontrunner of the day, during the GOP clown show, as well as your vehement opposition to any sensible legislation, along with the obstructionist Congress. Like the wolf crying boy, and the tinfoil hat wearing Trump, merely crowing at the conspiracy of the day, does not make a thoughtful opinion. I, on the other hand, have been correct about the aforementioned Clown Primaries, as well as the election. Only, I never incorrectly boasted about it, like your severely il;l infromed self. So, in the spirit of teat for tat, go dumpster diving your own self, I do not have time.
Posted 1354 days ago.
See Richard Blumenthal (D) who lied during a political campaign (2010) about serving in Vietnam. That's what a blatant military service lie looks like.
Now, get back in that dumpster, ya' little wienerschnitzel
Posted 1354 days ago.
Lindsey Graham, the effeminate ChickenHawk is a Gulf War ERA veteran, NOT a Gulf War Veteran. See the difference? He promotes himself as something he is NOT. That makes him a LIAR.
Posted 1354 days ago.
"Senator Lindsey Graham is a member of the Air Force Academy's Board of Visitors, having been appointed to the Board by the vice president in 2011.
Graham serves in the U.S. Air Force Reserves as a colonel assigned as a senior instructor at the Air Force Judge Advocate General School. He served on active duty for more than six years, including a four-year assignment at Rhein-Main Air Base, Germany, from 1984-1988.
Upon leaving active duty Air Force in 1989, Graham joined the South Carolina Air National Guard, where he served until 1995. During the first Gulf War in the early '90s, Graham was called to active duty and served state-side at McEntire Air National Guard Base, S.C., as Staff Judge Advocate where he prepared members for deployment to the Persian Gulf region."
(Source: USAFA)
Posted 1354 days ago.
Have you been reading through the "E" section of your dictionary?
First, I disagree with your vacuous ass-essment. Second, nothing "effeminate" about him. Third, your effluent efflorescence is mere virulent pus in bloom...or is that Puss N' Boots? Either way, you might qualify as either medical waste, or a polyester sock.
Go Occupy a dumpster.
Posted 1354 days ago.
The effeminate WarHawk LIED about being a WAR veteran, and got elected despite the lies. Curious. It just shows to go you how gullible the electorate can be. Now look at him. A GOP 'leader.
Posted 1355 days ago.
This morning we are treated to the fake, false Lindsey Graham, an effeminate WarHawk, who boasted of being a Gulf War Veteran (he was an Air Force lawyer and NEVER left South Carolina), during that conflict, blathering on about Benghazi.This is a leader? Thanx for the vote of confidence, Amos, but all politicians are liars, by my definition, and I refuse to compromise my integrity, so replacing Benishek will have to be by vote, so we will get him next time.
Posted 1355 days ago.
We should draft Jeffry Feiger to replace Snyder and Norman Lake to replace Beneshek.
Posted 1355 days ago.
"A businessman was confused about a bill he just received so he asked his secretary for some mathematical help. He asked, "If I were to give you $20,000 dollars minus 14% how much would you take off?"
She replied, "Everything but my earrings."
Posted 1356 days ago.
Simple solution: Adequate signage.
"Fraud Free Zone"
Posted 1357 days ago.
"The Hamilton County (OH) board of elections has issued 28 subpoenas involving double voting and general voter fraud in the 2012 Presidential election.
42 U.S.C. § 1973i(e) makes it a felony punishable by a fine of $10,000 or five years in prison for anyone to vote twice in a federal election.
Meloweese Richardson admits to voting for herself, her daughter and other family members. She is also a poll official in her Ohio neighborhood.
The vote fraud deniers and enablers are corrupting the sanctity of American elections. Academics like Justin Levitt at Loyola School of Law have ridiculed those of us who care about voter fraud, saying it is as rare as Sasquatch."
Obama won in every state that did not require a Photo ID and lost in every state that did require a Photo ID.
Posted 1357 days ago.
I apologize for making much less income while paying much more in taxes.
I don't qualify for Green Energy tax credits, Green Energy Dept. Of Energy loans, or any of that. I'm also not one of the dozens of companies to get Green Energy loans right before filing for bankruptcy.
It does motivate the heck out of me to pay through the nose in taxes, money that my family could certainly use, so that this administration can give it right to their crony capitalism friends.
Crony Capitalism...Green Energy. The Government doesn't create a darn thing. They shouldn't be punishing some companies while rewarding others.
Quid Pro Quo is out of the closet and wearing a very loud costume and dancing around...Gangnam Style.
Flat tax. Scrap the entire tax code.
Posted 1357 days ago.
The next four top Corporate Welfare Corps...Goldman Sachs paid 1.4% tax with a profit of 2.3 Billion and 800 Million from the Fed. CitiGroup 4 Billion profits, NO Tax, 2.5 Trillion Bailout from 'us'. ConocoPhilips, 16 Billion profit '07-'09 461 Million Tax break. Carnival Cruise Line 11 Billion profits, income tax 1.1%. Special interest for the Corporate Welfare State, trickle down pennies for the rest of us. Go get' em Bernie! and let the excuses and apologies begin from you know where.
Posted 1357 days ago.
The top 6 tax scoundrels of America...Exxon Mobil 19 Billion in profit in 2009, NO tax paid on income, with 156 Million rebate from 'our' IRS. Bank of America, 1.9 Tax refund, to go with 4.4 Billion Profit, along with a ONE Trillion Bailout. General Electric 26 Billion profit over last 5 years, to go with a 4.1 Billion refund from 'our' IRS. Boeing, with a 30 Billion contract from the Pentagon to build 179 airborne tankers 124Million tax refund from 'us'.Valero Energy 68 Billion sales, 157 Million Tax refund from 'us', along with a 3 year, 134 Million tax break for oil& gas tax deduction. Chevron 19 Million refund from 'us', last year from 'us' after 10 Billion in profits min '09. Thank You Bernie Sanders, for having the courage to bring this up, and doing something about Corporate Welfare.
Posted 1357 days ago.
It sure appears that the "stupid party" is comming apart with the rich fighting idiots who think earth is 9,000 years old. They will give us eight years of Hillary which is a bit scary.
Posted 1357 days ago.
"In a society of wolves, you do not fight back by creating more sheep!" (Dan Bongino: Former Secret Service Agent)
Why did the Swiss survive WW II yet millions of the Jewish population were put to death in Nazi gas chambers? A well armed Jewish population would have had a better chance of survival. They were disarmed first though.
Why does Obama and his followers want to confiscate citizen's weapons, yet has no problem arming Middle Eastern rebels and Mexican drug cartels? Please explain.
Those who allow history to repeat itself will become actors in the rerun. Watch the movie "Defiance" with Daniel Craig. It's a true story.
Posted 1358 days ago.
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text:
He needs a family with another energetic cat or cat-savvy kids who can handle his high activity level. A busy household would be a great environment for him. The more people around to play with him and love him, the better.
Cash does have feline immunodeficiency virus (FIV), but it's not contagious to people or non-cat pets, and he should live a long life. Cash does need to be either the only cat or can live with another FIV-positive cat.
To see Cash or any of the more than 200 other cats available for adoption, visit the Cat Adoption Team (C.A.T.), an adoption shelter, 14175 S.W. Galbreath Drive, Sherwood. Adoption hours: 11a.m.-7 p.m. Monday-Friday, 10 a.m.-6 p.m. Saturday-Sunday; 503-925-8903 or www.catadoptionteam.org.
Each week The Oregonian features an adoptable cat or dog from one of the local animal shelters. Featured animals are subject to prior adoption.


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With the approval of voters in hand, the Jenison Pubolic Schools Board of Education will meet March 7, instead of the regularly scheduled March 14, to propose a resolution to sell bonds. "We must get into the market as soon as it appears profitable," said Superintendent Tom TenBrink.
The new tax rate, approved on Feb. 22 to pay for improvements to buildings, technology, and to build a fine arts center, could be collected this summer, depending on when bonds are issued. The district will begin seeking bids this week for design work for the fine arts center, which will probably be be built in spring 2012.
The board meets at 7:30 p.m. at the Jenison High School media center.


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Thread: Authentication Proxy
08-19-2008, 12:06 PM #1Senior Member
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Authentication Proxy
Todd talks in his current CCNA book about authentication proxy. I was actually thinking of setting an FTP auth proxy up in my environment, but the Cisco documentation is unclear. Any chance someone could post an example? Do you have to use RADIUS or TACACS+, or could you use the built-in AAA in a current router?
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Here's our District 3-A girls' basketball rankings for the 2012-13 season. Records through Jan. 27. Rankings are independent of D3 power ratings used for seeding purposes.
1. Steel-High (15-2) – last week: 1 – In lone battle last week, Rollers produced 94 points against Capital Division foe Camp Hill. Big, and likely historic, week coming up for Jeff Chisholm's crew.
2. Lebanon Catholic (10-9) – last week: 2 – A pair of losses to Lancaster Mennonite and Lancaster Catholic hasn't diminished our faith in the Beavers one bit. In fact, we fully expect the L-L challenger to run the regular-season table and hit D3 play in stride.
3. Halifax (10-8) – last week: 3 – Although the Wildcats are still searching for more consistency, another triumph over Millersburg last week has allowed Halifax to remain in the Tri-Valley League title hunt.
4. Harrisburg Christian (11-5) – last week: 4 – Rebounded from tight loss with 3-AAA Donegal with three straight wins. A good indication of how far the Knights have traveled happens tonight against Camp Hill.
5. High Point (9-3) – last week: not ranked. It's time we handed High Point and those double-digit leaders, Tori Moll, Paige Hansen and Lorraine Piccirillo, a shot to defend the ACCAC entry.
Dropped out: Millersburg
Bible Baptist (11-6)
Millersburg (8-9)
Mount Calvary (13-4)
ON TWITTER: @threejacker


text:
TRC2004 Rugged Military Computer
- Front Access Storage Drives
- 18 Inch Enclosure Depth
- DP or UP uATX Motherboard
- PCI Express & PCI I/O Support
- Overview
- Tech Specs
- Downloads
- Options
The TRC2004 Rugged Military Computer
The TRC2004 rugged military computer is a ideal system solution for government & defense applications. The system's MicroATX (uATX) motherboard and the rugged computer design enables deployment across a wide spectrum of applications. The TRC2004 rugged military computer features a dual or single-processor motherboard with Intel® Xeon® processors.
Motherboard options include the dual-processor JXM7031 and the single-processor JXMS7031 or JXMI8001, all of which are designed and manufactured by Trenton Systems to ensure maximum longevity that results in extended 2U rackmount computer system configuration stability.
Additional 3rd party motherboards that are validated by Trenton for maximum performance are available with the TRC2004 rugged military computer. This 19″ rackmount computer features front access /hot swap storage drives in a shallow depth 18″ enclosure.
Key features of the TRC2004 rugged military computer include:
- Rugged, lightweight aluminum chassis
- Built to order system configuration flexibility
- Accommodates up to four hot swap, front access 2.5″ HDD/SDD storage drives
- Efficient chassis cooling for long-term system reliability
- Features I/O support for PCI Express and PCI plug-in cards
- Dual or single-processor MicroATX motherboard options feature multi-core processor
Like all of our rugged built-to-order computer systems, the TRC2004 is designed, integrated and supported by Trenton Systems in the United States. Our exclusive 5-year factory warranty on motherboards manufactured by Trenton is standard with this rugged military computer.
Tech SpecsMODEL NUMBERBOARD TYPE
The Trenton TRC2004 is designed for applications that require outstanding system performance in a stable computing platform having extensive data storage capacity. This rugged and yet lightweight (22.0 lbs/10.0 kg) industrial computer is constructed using a 2U, rackmount aluminum chassis with a shallow 18" depth for mounting in areas where component space is at a premium. The TRC2004 is configured with Trenton's exclusive dual-processor, MicroATX long-life motherboard (JXM7031) with an available configuration option that supports a single CPU. The dual-processor configuration supports one x16 PCI Express® 2.0/1.1 card as well as two additional x8 PCIe option cards. The system also supports a 32-bit/33MHz PCI option card.
The TRC2004 system solution supports up to four storage drives, a slim-line optical media drive and offers multiple power supply configuration options. The 18.0" chassis depth enables support for the wide variety of full-length/low-profile option cards typically used in diverse applications such as military systems, industrial automation machinery and medical diagnostics.DEPTH
18″/457mmCHASSIS STANDARD
Lightweight Rugged Aluminum EnclosureCOLOR
19″ Rackmount Chassis, 2U enclosure height, MicroATX long-life motherboard in a shallow-depth enclosure with front access HDD drive carriers, a slim-line optical drive, front panel USB ports with rear panel I/O ports and PCIe and PCI option card slots with a fixed or removable power supply optionDRIVE BAYS
• 2 – 3.5″ bays support 2 – 3.5″ fixed HDDs or 4 – 2.5″ front removable/hot swap HDDs (max)
• 1 – Slim-line optical drive bay
2 – Front access 3.5″ drive carriers for a SATA HDDs
4 – Front access 2.5″ drive carriers for a SATA HDDs
1 – Slim-line optical drive bayPOWER SUPPLY
1U rear-mounted and removable or 2U fixed rear-mounted ATX/EPS
1U rear-mounted and removable 400W ATX/EPS power supply
2U fixed rear-mounted 650W ATX/EPS power supply
Other supply options available upon request.CPU
Dual-Processor MicroATX Motherboard (JXM7031)
Features two, Intel® Xeon® C5500 Series long-life, quad-core or dual-core processors
Single-Processor MicroATX Motherboard (JXMS7031)
Features one, Intel® Xeon® C5500 Series long-life, quad-core or dual-core processor
Other industry standard MicroATX motherboards are supported in the TRC2004 system. These alternative motherboard options provide additional processor choices.OPTION CARD SLOT DETAILS
The following option cards slots are available when using the Trenton dual-processor JXM7031 motherboard in the TRC2004:
1- x16 PCI Express 2.0/1.1
2 – x8 PCIe 2.0/1.1
1- 32-bit/33MHz PCI
The following option cards slots are available when using the Trenton single-processor JXMS7031 motherboard in the TRC2004:
2 – x8 PCI Express 2.0/1.1
1- 32-bit/33MHz PCI
The TRC2004 rackmount computer supports low-profile option cards where H = 1.43" (36.07mm) to 2.54" (64.41mm).COOLING
3 – 80mm fans (front-mounted), 67CFM each, other system fan options availableINDICATORS
Front LEDs for HDD activity and power status and rear LED for LAN activityFRONT CONNECTOR PORTS
1 – Power On/Off
1 – System ResetAIR FILTER
Front access system filter for cleaning and maintenanceMETRIC DIMENSIONS
48.3cm (W) x 8.9cm (H) x 45.7cm (D)ENGLISH DIMENSIONS
19.0″ (W) x 3.5″ (H) x 18.0″ (D)CHASSIS NET WEIGHT
22.0 lbs. (10.0 Kg.) includes chassis, dual-processor MicroATX motherboard and power supply only
Note: Final system weight is a function of a your specific TRC2004 system configuration
DownloadsCHASSIS DRAWINGSDATASHEETCONFIGURATION DETAILS
Trenton Motherboards


text:
A diet-induced mouse model for glutaric aciduria type I
In the autosomal recessive human disease, glutaric aciduria type I (GA-1), glutaryl-CoA dehydrogenase (GCDH) deficiency disrupts the mitochondrial catabolism of lysine and tryptophan. Affected individuals accumulate glutaric acid (GA) and 3-hydroxyglutaric acid (3-OHGA) in the serum and often suffer acute striatal injury in childhood. Prior attempts to produce selective striatal vulnerability in an animal model have been unsuccessful. We hypothesized that acute striatal injury may be induced in GCDH-deficient (Gcdh−/−) mice by elevated dietary protein and lysine. Here, we show that high protein diets are lethal to 4-week-old and 8-week-old Gcdh−/− mice within 2–3 days and 7–8 days, respectively. High lysine alone resulted in vasogenic oedema and blood–brain barrier breakdown within the striatum, associated with serum and tissue GA accumulation, neuronal loss, haemorrhage, paralysis, seizures and death in 75% of 4-week-old Gcdh−/− mice after 3–12 days. In contrast, most 8-week-old Gcdh−/− mice survived on high lysine, but developed white matter lesions, reactive astrocytes and neuronal loss after 6 weeks. Thus, the Gcdh−/− mouse exposed to high protein or lysine may be a useful model of human GA-1 including developmentally dependent striatal vulnerability.
- striatum
- subarachnoid haemorrhage
- animal model
- blood–brain barrier
- encephalopathy
- 3-OHGA = 3-hydroxyglutaric acid
- BBB = blood–brain barrier
- GA = glutaric acid
- GA-1 = glutaric aciduria type I
- GCDH = glutaryl-CoA dehydrogenase
- GFAP = glial fibrillary acidic protein
- OHGA = hydroxyglutaric acid
- RITC = rhodamine isothiocyanate
- WT = wild-type
Glutaryl-CoA dehydrogenase (EC 220.127.116.11) (GCDH) activity is required for normal mitochondrial oxidation of glutaryl-CoA produced by lysine and tryptophan catabolism. Autosomal recessive inheritance of defective alleles of GCDH results in glutaric aciduria type I (GA-1) and a loss of normal breakdown of glutaryl-CoA detected as accumulation of glutaric acid (GA), 3-hydroxyglutaric acid (3-OHGA) and glutaryl-carnitine in tissue, plasma and urine (Goodman and Frerman, 1995; Kolker et al., 2003; Strauss et al., 2003; Funk et al., 2005).
The most striking feature of GA-1 is bilateral striatal degeneration of acute or chronic onset associated clinically with severe disabling dystonia and choreoathetosis. Histopathological findings include neuronal loss and astrogliosis in the striatum, subdural haemorrhages and spongiform vacuolation of the white matter (Goodman et al., 1977; Leibel et al., 1980; Chow et al., 1988; Bergman et al., 1989; Soffer et al., 1992; Kimura et al., 1994). Currently accepted therapy for GA-1 employs dietary lysine restriction through low protein diets to reduce GA-producing substrates and carnitine supplementation to prevent carnitine deficiency (Baric et al., 1998). Unfortunately, about one third of children with GA-1 suffer striatal degeneration regardless of adherence to therapy (Strauss and Morton, 2003). Design of more effective therapies aimed at protecting all affected children will require a better understanding of GA-1 pathophysiology.
A GCDH-deficient mouse was generated in order to study the pathophysiology of GA-1 (Koeller et al., 2002). The GCDH knockout mouse (Gcdh−/−) accumulates GA and 3-OHGA in serum, urine and tissue, similar to the human disease. Behavioural testing of these mice revealed mild motor deficits in otherwise healthy animals. The Gcdh−/− mice have not been shown to develop striatal degeneration typical of the human disease, either spontaneously or after application of metabolic or infectious stressors thought to trigger acute striatal injury (Koeller et al., 2002). Adult Gcdh−/− mice develop vacuolation primarily in the frontal cortex.
The rationale for lysine and protein restriction as therapy for GA-1 is based on the hypothesis that the accumulation of downstream metabolites is responsible for the pathogenesis of the disease. We asked if increased dietary protein, lysine or tryptophan would aggravate disease presentation in the Gcdh−/− mouse. In the present study we report neuropathological, biochemical and magnetic resonance imaging changes that are associated with developmentally dependent lethality and neuropathology induced by high protein or lysine diets.
Materials and methods
All chemicals were purchased from Sigma (St Louis, MO, USA) unless otherwise specified. 3-OHGA was prepared from the dimethyl ester precursor as previously described (Cohen and Khedouri, 1960). The concentration was confirmed by gas chromatography/mass spectroscopy with a deuterated internal standard.
Gcdh−/− mice and wild-type (WT) littermate controls, both of mixed C57Bl/6J × 129SvEv background (Koeller et al., 2002), were generated from heterozygotes and maintained at Penn State College of Medicine (Department of Comparative Medicine) in accordance with IACUC research guidelines set forth by Pennsylvania State University and the Society for Neuroscience Policy on the use of animals in neuroscience research. Male and female WT and Gcdh−/− mice from F1 and F2 generations were placed on special diets as 4-week-old weanlings (youngest available age to wean and place on special diets) or 8-week-old adults. Mice were sacrificed when they exhibited symptoms such as paralysis and seizures, or after 6 weeks. Mice were anaesthetized with 100 mg/kg pentobarbital (i.p.), perfused with lactate Ringer's followed by 4% paraformaldehyde for 15 min. Brains were removed and post-fixed for 48 h, and paraffin embedded.
To test blood–brain barrier (BBB) integrity, 4-week-old WT and Gcdh−/− mice were exposed to either normal or high lysine diets for 3 days (n = 3 each group). The mice were then perfused transcardially with 200 ml of 0.5% Evans blue in phosphate-buffered saline (PBS) with 500 units of heparin as previously described (Reynolds and Morton, 1998). Brains were removed and fixed for 48 h prior to dissection.
Special diets
All diets were purchased from Harland Teklad (Indianapolis, IN, USA). The high protein diet (TD.03637) (70% casein) contains 62%, rather than the 18% protein of standard diets (Harland Teklad 2018 rodent diet). This high protein diet is 4.7% lysine and 0.7% tryptophan. The high lysine diet (TD.04412) was prepared by adding free lysine to a standard diet to achieve 4.7% total lysine (5-fold of the normal diet). The high tryptophan diet (TD.04411) was prepared by adding free tryptophan to achieve 1.5% total tryptophan (7.5-fold normal). These lysine and tryptophan levels are below those previously shown to cause adverse effects in normal animals (Benevenga and Steele, 1984; Tsubuku et al., 2004).
Behavioural evaluation
All special diet treated animals and corresponding controls were evaluated for motor deficits using a quantitative neurological scale developed previously (Ouary et al., 2000). Motor abnormalities were scored on the presence and severity of motor symptoms consisting of dystonia (intermittent dystonia of one hindlimb, score = 1; intermittent dystonia of two hindlimbs, score = 2; permanent dystonia of hindlimbs, score = 3), gait abnormalities consisting mainly of uncoordinated, wobbling gait (score = 1), recumbency (animals lying on one side and showing uncoordinated movements when stimulated, score = 5; near death recumbency characterized by almost complete paralysis with rapid breathing, score = 6). In addition, the capability of the animals to grasp, for a few seconds, a cage grid with their forepaws (able = 0; unable = 1) and their hindpaws (able = 0; unable = 1) was assessed. The presence of these motor abnormalities was studied on a daily basis and a neurological score was calculated for each animal as the sum of all indices (minimum = 0, normal animal; maximum = 8, animal showing near death recumbency). Body temperature was measured rectally using a SAII temperature monitor (SA Instruments, Stony Brook, NY, USA). Dehydration was evaluated by weight and skin redundancy. Animals that lost 20% of their body weight within two days and exhibited skin redundancy were considered dehydrated.
Biochemical analysis
Amino acids
Serum amino acids were separated and quantified as previously described (Morton et al., 2002) using a Hewlett-Packard (Palo Alto, CA, USA) 1090 series II HPLC and an Amino Quant column containing ODS-Hypersil.
Organic acids
Deuterated internal standards for GA (d4-GA) (0.0092 µmol) and 3-OHGA (d5-HGA) (0.0023 µmol) were added to 80–120 mg of brain tissue or to 20 µl of serum. Brain tissue was homogenized in 100 µl of 10 N NaOH (pH >10) and heated for 30 min at 80°C. A single 1 ml ethyl acetate extraction was used to remove cholesterol and neutral lipids. Saturated salt solution (200 µl) and 28% HCl (100 µl) were added to the remaining aqueous phase. The brain samples were extracted in 1 ml of methylene chloride to remove long-chain fatty acids and phospholipids. Serum and brain samples were extracted into 3 ml of ethyl acetate, dried under nitrogen and derivatized with 100 µl of BTSFA at 80°C for 20 min. Samples were diluted with 100 µl of ethyl acetate and 1 µl was injected onto a Hewlett-Packard 6890 gas chromatograph with a Hewlett-Packard 5973 mass spectrometer. The mass spectrometer measured total ion current for selected ions 158 and 261 (GA), 349 and 185 (3-OHGA), 265 (d4-GA), and 354 (d5-HGA) over appropriate retention times.
Liver enzyme tests
Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) analyses were performed by the Department of Comparative Medicine using a CX7 Synchron automated analyser (Beckman Instruments, Fullerton, CA, USA).
MRI was performed on a 3.0 T Medspec S300 (Bruker Instruments, Ettlingen, Germany) using a 2.5 cm slotted tube resonator. MR images were obtained from 4-week-old (n = 9) and 8-week-old (n = 5) Gcdh−/− mice, and 4-week-old WT (n = 3) prior to and 3–5 days following high lysine diet exposure as they developed symptoms (hypothermia, hypoactivity, paralysis and seizures). After the baseline scan, Gcdh−/− mice on the high lysine diet were imaged once they appeared hypoactive (neurological score ≥ 3) and 1–3 additional times depending on survival with at least 48 h between scans. WT 4-week-old mice did not have any apparent behavioural deficits and were imaged after 3 weeks of high lysine exposure. Adult (8-week-old) Gcdh−/− mice remained asymptomatic and were imaged after 6 weeks of high lysine, and sacrificed afterwards. Prior to imaging, mice were anaesthetized with 2 mg/kg xylazine and 15 mg/kg ketamine (i.p.). Each animal was imaged with a T2-weighted multi-echo spin echo sequence (ten 0.5 mm thick slices, TR/TE = 3000/10–152 ms, 15 echoes, 156 × 78 µm2 resolution, 2 averages) and diffusion-weighted imaging (four 1 mm thick slices, 0.5 mm slice separation, TR/TE = 1500/70 ms, Δ = 30 ms, δ = 16 ms, b-value = 1030, 208 × 208 µm2 resolution, 1 average, 288 s total imaging time). Transverse relaxation time constant (T2) was calculated on a pixel-by-pixel basis from the exponential fit using CCHIPS software (Schmithorst et al., 2001). Calculated T2-values are displayed as colour-coded map. Following imaging, mice were kept at 37°C for 1 h and allowed to recover.
H&E slides were prepared from WT and Gcdh−/− mice 4–6 weeks and 12–14 weeks of age on normal or high lysine diets. Three animals were used from each group. Coronal 10 µm thick brain slices were made within 0.5 mm of the bregma line for striatal sections and between bregma −1.5 and −2.0 for hippocampal sections.
Glial fibrillary acidic protein (GFAP) and neuron-specific nuclear protein (NeuN) were detected using deparaffinized 10 µm thick coronal brain slices. Tissue was blocked with normal serum and incubated with polyclonal α-GFAP (1:500) (Dako, Carpenteria, CA, USA) or monoclonal α-NeuN (1:20) (Chemicon, Temecula, CA, USA) in PBS overnight at 4°C. The slides were washed three times in PBS and processed further using Vectastain ABC kits (Vector Labs, Burlingame, CA, USA) for α-rabbit (GFAP) or α-mouse (NeuN) as per the manufacturer's instructions and developed with DAB. GFAP slides were counterstained with cresyl-violet.
Neuron counts
Neurons were counted under 10× magnification of 10 µm thick sections stained for NeuN as described above. Coronal sections at bregma zero were used from Gcdh−/− normal diet controls (n = 3), lysine treated for 3 days at 4 weeks of age (n = 5), and lysine treated for 6 weeks at 8 weeks of age (n = 5). Brain areas were determined according to 'The Mouse Brain in Stereotaxic Coordinates' (Franklin and Paxinos, 1997). For cortex a 500 µm × 600 µm rectangular area from each hemisphere was selected 2 mm lateral to the mid-line with the top edge parallel to the cortical surface and extending ventral to encompass each cortical layer. For striatum a 500 µm × 600 µm rectangular area from each hemisphere was selected between 2–3 mm from the mid-line and 2–3 mm from the base of the brain. Neurons were considered NeuN positive cells with identifiable nuclei.
Striatal/cortical microvessel cultures
Rat brain microvessels were isolated as previously described (Antonetti and Wolpert, 2003) with some modifications. Male Sprague-Dawley rats (n = 11) (Taconic, Germantown, NY, USA) were anaesthetized to remove brains. Striatal and cortical parts were pooled separately after removing meninges and the choroid plexus. Tissue was Dounce-homogenized in Dulbecco's modified Eagles's medium (DMEM) (Gibco, Rockville, MD, USA) and passed over nylon mesh. The mesh fraction was washed with DMEM and collected. Separate striatal and cortical fractions were grown in MCDB-131 media supplemented with 10% foetal bovine serum, 10 ng/ml epidermal growth factor, 0.2 mg/ml ENDO GRO (VEC Technologies, Inc., Rensselaer, NY, USA), 0.09 mg/ml heparin, and antibiotic/antimycotic (Gibco, Rockville, MD, USA). Cultures were grown in a humidified incubator at 37°C under air/CO2. Monolayer cultures were assessed for morphology by light microscopy and expression of ZO-1 (endothelial cell marker) by western blotting (data not shown).
Striatal/cortical microvessel permeability
Second and third passage striatal or cortical microvessel cultures were used to make monolayer cultures in 10.5 mm PET transwell filter plates with a 0.4 µm pore size (Becton-Dickinson, Franklin Lakes, NJ, USA) as previously described (Antonetti and Wolpert, 2003). Monolayers were confluent by the second day. GA or 3-OHGA was added at 3300 µM each. After one hour 10 µM 70 kDa rhodamine isothiocyanate (RITC)-dextran was added to the apical chamber and 100 µl aliquots were removed from the basolateral chamber at 15, 30, 45 and 60 min. The aliquots were quantified on a Fluorimager 595 (Molecular Dynamics) and P0 values (diffusive permeability) were calculated as previously described (Chang et al., 2000).
Cell viability
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2-H-tetrazolium bromide] reduction (Roche Molecular Biochemicals, Indianapolis, IN, USA) was used to assess differences in cell viability of striatal or cortical monolayer cultures used in the RITC-dextran flux assays after 6 h of continued treatment.
Normally distributed datasets were analysed by ANOVA with Fisher LSD post hoc test. Kruskal–Wallis one-way analysis of variance on ranks was performed with Student-Newman-Keuls post hoc test on samples that were not normally distributed. Sigma Stat software (Jandel Scientific, San Rafael, CA, USA) was used for analysis. A P-value < 0.05 was considered statistically significant.
Survival with special diets
High protein was lethal to all Gcdh−/− mice (Fig. 1A). All 4-week-old Gcdh−/− mice died within 2–3 days of high protein diet exposure and 8-week-old Gcdh−/− mice survived slightly longer, all dying after 7–8 days. WT animals appeared normal through the 90-day test period of high protein diet exposure (Fig. 1A).
Exposure to a high lysine diet had lethal consequences for 15 of 20 (75%) 4-week-old Gcdh−/− mice, while only 1 of 9 (11%) 8-week-old Gcdh−/− mouse died following 7 days of the same high lysine diet (Fig. 1B). The remaining Gcdh−/− mice in both age groups and all isogenic WT controls survived longer than 90 days on high lysine. All Gcdh−/− and WT mice in either age group survived and remained asymptomatic on high tryptophan longer than 90 days (data not shown).
Gcdh−/− mice that eventually died from high protein or high lysine as well as some that survived high lysine were the only ones to manifest obvious symptoms of toxicity. For 4-week-old Gcdh−/− mice on the high lysine diet, the onset of symptoms varied 1–2 days, with a common pattern of progression: within 2–4 days of starting the diet, Gcdh−/− mice became hypothermic (28–32°C n = 8, 32–34°C n = 4); after 2 more days, mice became dehydrated and hypoactive (neurological score ≤ 3), followed by paralysis and seizures within 24 h (neurological score > 5), followed in turn by death within 12 h. Attempts to hydrate some of these animals with subcutaneous saline injections did not alter the course of symptoms or death. Two of the five surviving 4-week-old Gcdh−/− mice on high lysine became hypothermic and dehydrated, but never exhibited abnormal movements or paralysis and eventually recovered. No Gcdh−/− or WT mice exhibited symptoms with high tryptophan or normal diets.
Biochemical analysis with special diets
Serum GA levels in 4-week-old Gcdh−/− mice on a normal diet were 150 ± 5 µM (mean ± SEM). The high lysine diet raised serum GA to 2481 ± 219 µM (mean ± SEM) only in animals that displayed severe symptoms (paralysis and seizures). Asymptomatic animals maintained serum GA levels of 269 ± 48 µM (mean ± SEM), which was 80% higher than Gcdh−/− mice on a normal diet (Fig. 2A). Organic acid analysis of brain tissue from 4-week-old Gcdh−/− mice fed high lysine revealed GA levels of 3267 ± 985 µmol/kg wet weight (mean ± SEM) only in animals that had severe symptoms. Gcdh−/− mice that remained asymptomatic on high lysine had brain GA levels of 641 ± 171 µmol/kg wet weight (mean ± SEM), which was a 68% increase over 385 ± 113 µmol/kg wet weight (mean ± SEM) found in Gcdh−/− mice on the normal diet (Fig. 2B). No significant accumulation of 3-OHGA was found in serum or brain samples from Gcdh−/− mice exposed to the high lysine diet (data not shown).
Asymptomatic and symptomatic Gcdh−/− mice as well as all WT mice had significant (P < 0.03) serum lysine accumulation of 1257 ± 240 µM (mean ± SEM) after being placed on the high lysine diet, compared with mice on normal or tryptophan diets with serum lysine of 609 ± 187 µM (mean ± SEM) (Fig. 2C). Serum lysine of normal rodents is 245 µM (Smith, 2000). There was no difference in serum lysine accumulation between WT, symptomatic and asymptomatic Gcdh−/− mice on high lysine; therefore unlike GA accumulation, serum lysine accumulation did not correlate with disease. Symptomatic Gcdh−/− mice on the high lysine diet did have serum branched chain amino acid accumulation. Leucine, valine and isoleucine serum concentrations were significantly elevated (888 ± 3 µM, 925 ± 50 µM and 323 ± 7 µM; mean ± SEM) in the symptomatic Gcdh−/− mice placed on the high lysine diet at 4 weeks of age (Fig. 2D). In normal mice, serum leucine, valine and isoleucine concentrations are 164, 222 and 103 µM, respectively (de Boer et al., 2002). AST values were slightly elevated in symptomatic Gcdh−/− mice (519 ± 12 mean ± SEM) compared with asymptomatic Gcdh−/− mice (309 ± 34 mean ± SEM) on the high lysine diet. There were no significant elevations or differences for ALT values and there was no obvious liver pathology (data not shown). There were no other significant correlations between diet and amino acid accumulation in the serum, including tryptophan (data not shown).
MRI findings were normal for all WT mice 3 weeks following high lysine exposure (Fig. 3A). In contrast, all symptomatic Gcdh−/− mice exhibited loss of cerebral ventricles and signal hyperintensity in deep cortical laminae and striatum on diffusion-weighted and T2-weighted images 5–9 days after high lysine exposure (Fig. 3B). Three Gcdh−/− mice that were symptomatic after 10 days of high lysine had elevated T2-values in the cortex and elevated T2-values in the striatum compared with WT (P < 0.02) (Table 1). MRI of a symptomatic Gcdh−/− mouse with paralysis revealed bilateral striatal injury evidenced by highly elevated T2-values (∼105 ms) and diffusion-weighted signal hyperintensity in the striatum, deep cortical laminae and lateral hypothalamus after 12 days of high lysine diet exposure started at 4 weeks of age (Fig. 3C). Acute haemorrhage was present in this and three other Gcdh−/− mice prior to death (Fig. 3C, red arrow). Adult (8-week-old) Gcdh−/− mice were imaged prior to and 6 weeks following exposure to the high lysine diet. All 8-week-old Gcdh−/− mice developed bilateral signal hyperintensity in the caudate/putamen (Fig. 3D), with significantly increased T2-values in the striatum and cortex compared with WT (P < 0.001) (Table 1). No abnormalities were found in Gcdh−/− mice on normal diets for up to 6 weeks.
|Animal type and the diet
|T2-values (ms)
|WT, 4-week old on high lysine for 3 weeks (n = 3)
|72.27 ± 0.72
|73.2 ± 0.62
|Gcdh−/−, 4-week old on normal diet (n = 3)
|73.07 ± 0.96
|74.23 ± 1.86
|Gcdh−/−, 4-week old on high lysine for 10 days (n = 3)
|76.4 ± 2.72
|82.33 ± 2.41*†
|Gcdh−/−, 8-week old on high lysine for 6 weeks (n = 3)
|77.07 ± 0.29*†
|88.47 ± 1.65*†
Gross dissection of brains from 4-week-old Gcdh−/− mice on high protein or lysine for 3–6 days revealed global vascular congestion and subarachnoid, subdural and intraventricular haemorrhages (Fig. 4). No obvious thrombus or other obstructions were found within the congested vessels of this group.
Microscopic examination of brains from Gcdh−/− mice on high lysine confirmed the ventricular obliteration shown by MRI (Fig. 5, middle). Higher magnification of the cortex and striatum revealed large irregular vacuoles and dilation of the capillary bed compared with normal diet fed animals (Fig. 5, left). Gcdh−/− mice on a normal diet had moderate vacuolation in the cortex that was less severe in the striatum, as previously reported (Koeller et al., 2002) (Fig. 5, left). Gcdh−/− mice surviving on high lysine for 6 weeks showed severe vacuolation of the white matter patches of the striatum bilaterally (Fig. 5, right). Moderately increased vacuolation was also found in the deeper laminae of the cortex. This pattern of increased vacuolation correlated with the location of elevated T2-values in these animals (compare Fig. 5, right, with Fig. 3D). There were no differences observed between WT animals on high lysine and normal diets.
Neuronal loss
Severe disruption of cortical architecture with neuronal loss, confirmed by NeuN positive cell counts, was found in Gcdh−/− mice exposed to high lysine for 3 days (Fig. 6). Gcdh−/− mice exposed to high lysine at 8 weeks of age for 6 weeks showed neuronal loss in the cortex and striatum, confirmed by NeuN positive cell counts, and severe vacuolation of striatal patches (Fig. 6).
GFAP staining of brains from 8-week-old Gcdh−/− mice on the high lysine diet for 6 weeks revealed gliosis of the cortex and hippocampus that was less obvious in the striatum (Fig. 7, right). The 4-week-old Gcdh−/− mice on the high lysine diet for 3 days developed some gliosis in the same areas, which was less severe compared with the 8-week-old Gcdh−/− mice exposed to lysine for 6 weeks (Fig. 7, middle). Gliosis was absent from WT and Gcdh−/− mice on a normal diet and WT mice on high lysine for 6 weeks (Fig. 7, left, WT data not shown).
Blood–brain barrier breakdown
Evans blue stain was found in the choroid plexus and circumventricular organs in all animals of each group tested. Gcdh−/− mice placed on high lysine at 4 weeks of age for 3 days showed extravasation of Evans blue in the striatum, indicating BBB breakdown. Evans blue was found to escape from smaller vessels of the striatum (Fig. 8, right). Some larger vessels in the striatum of this group had concentrated blue staining and vasodilatation consistent with congestion. These observations were absent from the cortex in this group. WT mice on high lysine and Gcdh−/− mice on a normal diet, both 4 weeks of age, showed no evidence of BBB breakdown (Fig. 8, left and middle).
Increased striatal microvessel permeability with GA and 3-OHGA
To investigate whether GA or 3-OHGA can induce breakdown of the BBB, a previously studied model (Antonetti and Wolpert, 2003) of rat striatal and cortical microvessels was isolated and permeability of monolayers to RITC-Dextran was measured. Striatal microvessels were significantly more permeable to RITC-dextran flux after treatment with 3300 µM GA or 3-OHGA compared with cortical microvessels (P < 0.03) and controls (P < 0.001) (Fig. 9A). Increased permeability was not due to significant endothelial cell death. There were no significant differences in cell viability of monolayer cultures between any treatment groups and controls after 6 h of continued treatment (Fig. 9B).
The lack of severe spontaneous neuropathology in Gcdh−/− mice provides an opportunity to test environmental manipulations that may trigger the neuropathology seen in human GA-1. We hypothesized that increased dietary lysine in the context of GCDH deficiency would result in increased GA accumulation and trigger neuropathology in the Gcdh−/− mouse. Here we provide the first evidence that high protein and high lysine diets can cause neuropathology and death in a mouse model of GCDH deficiency. By applying these diets to weanling and adult Gcdh−/− mice, we have uncovered developmentally dependent lysine toxicity and two distinct types of injury based on age and length of diet exposure. Weanling Gcdh−/− mice placed on high lysine at 4 weeks of age present with an acute onset of symptoms including paralysis and seizures, associated with large accumulation of GA in serum and brain. Gross and microscopic examination of brains from these mice reveal BBB breakdown in the striatum and haemorrhages. MRI examination of a paralysed young Gcdh−/− mouse on the high lysine diet revealed acute bilateral striatal damage resembling the characteristic injury of human GA-1 (Baric et al., 1998; Strauss and Morton, 2003). In contrast, all the 8-week-old adult Gcdh−/− mice that survived and remained asymptomatic on high lysine for 6 weeks developed specific striatal lesions, evident as hyperintense T2- and diffusion-weighted signals. Microscopic examination of the brains from this group revealed disruption of white matter patches resulting from intense vacuolation within the striatum. The striatal changes were accompanied by reactive astrocytes in the cortex and hippocampus and neuronal loss in the cortex and striatum. These findings are consistent with the recently described adult onset GA-1 leucodystrophy (Bahr et al., 2002). The results of high lysine and protein diet exposure in young Gcdh−/− mice strongly support the use of protein and lysine restriction as standard therapy in human GA-1. Furthermore, the detrimental effects of high protein and lysine on adult Gcdh−/− mice support the suggestion that GA-1 patients maintain life-long dietary restriction (Bahr et al., 2002; Kolker and Hoffmann, 2003).
Brain GA accumulation correlates with neuropathology
Brain GA levels correlated with and may be responsible for the severe neuropathology in young Gcdh−/− mice exposed to the high lysine diet. Gcdh−/− mice that developed severe symptoms of paralysis and seizures or died after high lysine exposure accumulated brain GA levels above 1600 µmol/kg wet wt (∼2 mM based on 80% water content). The level was consistently below 800 µmol/kg wet wt (<1 mM) in asymptomatic mice. Symptomatic and asymptomatic Gcdh−/− mice on the high lysine diet accumulated similar concentrations of serum lysine. Gcdh−/− mice were not found to accumulate significant 3-OHGA. Therefore, GA accumulation correlates with disease and lysine accumulation does not.
Extracellular exposure versus intracellular accumulation of GA
Previous studies of GA exposure to organotypic slice cultures (Ullrich et al., 1999) and immature oligodendrocytes (OLN-93 cells) (Gerstner et al., 2005) provide evidence that extracellular GA exposure to immature oligodendrocytes may explain observed vacuolation seen in human GA-1 and Gcdh−/− mice. Here we provide evidence that extracellular GA exposure (3.3 mM) can disrupt BBB properties of striatal endothelial cells. Conversely, studies utilizing the extracellular approach of GA exposure to neuronal cell cultures or intrastriatal injections in vivo have failed to demonstrate repeatable neurotoxicity at disease relevant concentrations that can be applied as a model of neuronal loss in GA-1 (Lima et al., 1998; Ullrich et al., 1999; Kolker et al., 2000; Funk et al., 2004; Gerstner et al., 2005). The neuropathology found in Gcdh−/−mice exposed to high lysine may represent the effects of intracellular GA accumulation generated from increased lysine metabolism in the context of GCDH deficiency. GA generated within cells may cause toxicity by disrupting normal cellular processes. Induction of mitochondrial permeability transition, inhibition of glutamate decarboxlyase and alpha-keto-glutarate dehydrogenase as well as glutathione depletion have all been demonstrated at GA concentrations below those found in symptomatic Gcdh−/− mice on high lysine (Stokke et al., 1976; Palmeira et al., 2000; Sauer et al., 2005). These effects would require GA accumulation within cells or subcellular compartments. Alternatively, the process of producing GA may be toxic. Rapidly generated glutaryl-CoA from increased lysine oxidation within mitochondria may temporarily sequester limited CoA from vital citric acid cycle reactions, reducing available ATP. Accumulation of glutaryl-carnitine with carnitine depletion observed in GA-1 patients supports the hypothesis of CoA sequestration. Carnitine exchange for CoA is required to extract glutarate from mitochondria and free up the limited CoA pool (Russell et al., 1995; Ramsay and Zammit, 2004). The differential toxicity between the high protein and the high lysine diet, having the same lysine content, further supports the hypothesis that the process of generating GA may be toxic. A high protein diet can induce increases in lysine oxidation. Blemings et al. (1998), showed that a 60% casein diet can double lysine oxidation over a 20% casein diet in rat liver homogenates (casein is 87% protein). A 70% casein diet would be expected to more than double the lysine oxidation over the high lysine diet having only 18% protein. However, we cannot rule out the possibility of a synergistic effect of increased lysine and tryptophan together or another amino acid causing the observed severity for all Gcdh−/− mice on the high protein diet. Within the brain it remains to be shown in which cell types, in what form (GA, glutaryl-CoA, glutaryl-carnitine, glutaryl-glutathione) and how much GA accumulates as well as possible uptake, transport or excretion between cells and how production and elimination of GA are regulated.
Vascular injury
While hypotheses of neurotoxicity in GA-1 have been widely studied, they fail to demonstrate mechanisms that can parallel the rapid progression and regional specificity of acute striatal necrosis (Funk et al., 2004; Kolker et al., 2004; Funk et al., 2005; Strauss, 2005). Vascular injury as a paradigm of pathogenesis in GA-1 has been far less explored and can account for the rapid progression of striatal necrosis. However, models of vascular injury including bilateral carotid occlusion (Canese et al., 1997) or induced renal hypertension (Del Bigio et al., 1999) do not show the degree of symmetry found in GA-1. Distended blood vessels, BBB breakdown and haemorrhages indicate a vascular component of this disease, as previously suggested for humans (Haworth et al., 1991; Osaka et al., 1993; Kimura et al., 1994; Woelfle et al., 1996; Hartley et al., 2001; Strauss et al., 2003; Muhlhausen et al., 2004; Funk et al., 2005). Our results reveal BBB breakdown as an early pathophysiological event for young Gcdh−/− mice exposed to the high lysine diet. Furthermore, our permeability studies support regional specificity by demonstrating a difference in the ability to maintain barrier properties between striatal and cortical derived microvessels when exposed to GA levels (3.3 mM) similar to those found in brains of Gcdh−/− mice on the high lysine diet and GA-1 patients (Funk et al., 2005). Although the effect of 3-OHGA on striatal microvessels was similar, this compound has not been found at these concentrations in brain of human or mice with GCDH deficiency (Koeller et al., 2002; Funk et al., 2005).
Branch chain amino acid accumulation was found in serum of symptomatic Gcdh−/− mice on the high lysine diet and suggest an acute phase reaction of liver damage (Tarasow et al., 2003). However, ALT levels and liver histology, of young symptomatic Gcdh−/− mice after high lysine exposure, are not comparable with a mouse model of hepatic encephalopathy (Matkowskyj et al., 1999).
In conclusion, our results indicate that the Gcdh−/− mouse may indeed be a good model to study the development of neuropathology that characterizes human GA-1. Dietary protein, specifically lysine restriction, is strongly supported as standard therapy for GA-1, and life-long dietary restriction should be considered. This model may be used to further elucidate the underlying pathophysiology of this disease, which may in turn lead to effective ways of protecting children identified by newborn screening from the devastating clinical sequelae of striatal damage.
The authors dedicate this work to the memory of Dr Linda Crnic, who contributed significantly to the development of the Gcdh knockout mouse and also generated the mice used in this work. The authors thank Dr Kevin A. Strauss and Dr D. Holmes Morton for help with biochemistry and thoughtful guidance and suggestions in the course of their work. The authors also thank Lindsay Rush for help with preparation of figures. This work is supported by the Laverty and Oxford Foundations. M.B.S. acknowledges the support of NIH R01 EB000454.
- © The Author (2006). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: email@example.com


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Welcome to the city's best Brazilian Churrascaria restaurant in Vietnam!
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New York continued to see more people move from the state in 2013 than relocate here, according to an analysis from Atlas Van Lines.
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On a short streetcar ride to Poydras Street, few people talked. The OSU fans sat on one side of the car, the LSU backers on the other.
Gone are the friendly back-and-forth chants from both sides. It's apparent the game is only hours away.
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John Martin scored the go-ahead goal in the second period and Zack Bryan recorded four assists to lead Robbinsville to a 4-3 victory over Middletown North at Loucks Arena in Lawrenceville.
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