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Limited openings with MNC for Blended process ( 0 - 1 yrs)
BPO Talent Squad
RS 2,50,000 To 3,75,000 Mira-Bhayandar, Mumbai, Mumbai Suburb, Thane, Navi Mumbai
Dear Candidate, Currently we have Openings in Voice process for Day and Night Shifts. Designation: - Associate / Senior Associate / Specialist / Customer Service Representative Department: - Operations Location: - Thane JOB D...
Key Skills : customer care agent,customer associate,customer care executive,bpo executive,back office assistant,backend executive,back-office operations,service...
Talent Acquisition Consultant ( 0 - 1 yrs)
RS 1,00,000 To 1,25,000 Thane
Job Description We are hiring Consultant-Talent Acquisition/Senior Consultant – Talent Acquisition for our Thane Office Salary: Best as compared to industry standards Industry: Recruitment / Staffing Functional Area: HR, Rec...
Key Skills : communication skills,talent acquisition,talent acquisition specialist
AceTalent Consulting
RS 1,00,000 To 3,00,000 Mumbai
Job Description Job Role & Responsibilities Handling end-end recruitment (Screening, shortlisting, conducting initial telephonic discussion, processing to client).. Requirement Understanding & Identifying their needs.. Understandi...
Key Skills : recruiter,recruitment associate,recruitment specialist,human resource management,hr assistant,hr generalist,hr recruiter
Manas Sarovar
RS 50000 To 1,00,000 Pune
Jd : opening is for Mantra Resort At Bhor District 50 Kms, from Pune. overall Hr Admin Activities Related to the Resort. will have to Stay At the Resort employee Welfare, Time Keeping, Admin Activities, Co - Ordination with the Corporate Off...
Key Skills : hr executive,hr assistant,hr associate,hr generalist,hr recruiter,hr specialist
IT and HR Training Combo Offer for all Candidates at OSK... ( 0 - 1 yrs)
Osk Training and Placement
RS 50000 Nagpur
OSK We providing training with internship and live project in IT OSK Job Placement... Live Project Training with Internship for all Freshers/Pursuing or passed out Student in BBA/BCA/BE / BSc/DIPLOMA-CS,IT Combo package for all Students to l...
Key Skills : hr trainee,it engineer,pmis specialist,ict application engineer
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Register with us now to apply to these jobs
Walk-in for Hr Executive ( 0 - 1 yrs)
Aexonic Technologies Pvt Ltd
RS 1,00,000 To 1,25,000 Pune
How to Apply: To get reply Only Mail your application on sansoftinfotech@rediffmail or yahoo.com [Others will not get replJob Title: HR Specialist Why US? We are a small, energized team who has honed our skills across different verticals in s...
Key Skills : hr recruiter,payroll management,hr managememnt,erp-enterprise resource planning,erp operations,crm...
Walkin and take away the offer letter for IT freshers ( 0 - 1 yrs)
PC Technology Pvt Ltd
RS 1,00,000 To 2,00,000 Thane
Looking for Young, Talented and Expertise It Freshers Having Excellent Hands Over Java, .net, Php, Testing Platforms. qualification Required: bcs, Bca, Mca, Be Btech (it,Computers and Extc with Specialization Courses in Java,Php,Dot Net and Testi...
Key Skills : it engineer,it executive,it associates,java,pmis specialist,ict application...
Staff Nurse Required in Reputed Multi Specialist Hospital in Thane / Mumbai ( 1 yrs)
Rainbow Manpower (hr) Pvt Ltd
RS 1,75,000 To 3,00,000 Mumbai, Thane
(We Are Authorized Recruitment Channel Partner Of Reputed Hospital in Mumbai / Thane) WE provide Hospital jobs on Hospital payroll NOTE-we required good English communication skill candidate - Experience -1 to 3 yrs - Salary -As per Ind...
Key Skills : Staff nurse,B.sc nurshing,Nurse,nurshing
Sr. HR Officer - Core HR and Recruitment (IT Company) ( 1 yrs)
Logos Solutions
Experience: 1 to 3 years working in IT Company Job description - PF Formalities, Recruitment, Payroll, Core HR, Training and Development, Performance Appraisal, Legal Formalities.
Key Skills : hr officer,hr coordinator,hr specialist,hr supervisor
Area Sales Manager ( 0 - 3 yrs)
Pnp Polytex Pvt. Ltd
RS 2,25,000 To 6,00,000 Guwahati, Ahmedabad, Bangalore, Mumbai, Jaipur, Lucknow, Kolkata, Delhi, Hydrabad
B2b and B2c Selling. to Interact with Architects & Interior Designers Both Residential and Commercial and Builds a Strong Relationships with Them. to Set Up Dealers & Distributors Networks. to Handle the Core Strategies and Operation Teams to Gro...
Key Skills : area sales executive,area sales incharge,area sales manager,area marketing manager,business development manager
Magneto IT Solutions Pvt. Ltd
RS 1,00,000 To 2,50,000 Pune
Dear Candidates, Greeting from Magneto IT Solutions..!! Job Title: Sr. SEO Executive Salary: Commensurate with Experience Location: Ahmedabad RESPONSIBILITIES *Review and analyze client sites for areas that need to improved, deleted, ...
Key Skills : seo executive,senior seo executive,seo specialist
Hiring HR Recruiter ( Fresher)
RS 1,00,000 To 2,50,000 Thane
- End to End Recruitment. - Sourcing Candidates Through Various Job Portals. - Taking the Telephonic Round of the Interview. - Maintaining Proper Feedback.
Key Skills : hr associate,hr consultant,hr recruiter,hr specialist
Job Opening for the Post of Asst Mgr Hr with Our Client At Goregaon ( 3 - 10 yrs)
Prism Manpower Services
RS 3,00,000 To 6,00,000 Mumbai, Mumbai Suburb, Navi Mumbai
We have a job opening for the post of Asst Mgr HR with our client at Goregaon,Mumbai. Please below the job description for your kind perusal. Job Description : Designation : Human Capital & Corporate Communications Assistant Manager R...
Key Skills : human resource,human resource management,hr head,hr manager,asst hr manager,hr officer,hr specialist,mba,mba in hr
Required HR Consultant ( 1 yrs)
Napean India Outsourcing
RS 90,000 To 2,00,000 Nashik
1) Handling End to End Recruitment. 2) Sourcing, Screening, Taking Care of the Documentation. 3) Follow Up with the Selects. 4) Data Management. 5) Joining & Exit Formalities 6) Provide Training to the New Employees 7) Vendor Management 8)...
Key Skills : recruiter,recruitment,bpo,kpo,hr consultant,hr coordinator,hr specialist
Senior Manager/AGM - Talent Acquisition ( 6 - 10 yrs)
Talent Leads Consultants
RS 7,00,000 To 9,00,000 Mumbai
Strategic Talent Acquisition role - Employer Branding / Campus Recruitment - HR Projects - Lead delivery of end to end Recruiting service across all hiring levels, including branding, sourcing, and contracting - Manage resource alloca...
Key Skills : hr executive,hr manager,hr,he recruiter
Sr HR Manager - Strategic Talent Acquisition ( 4 - 6 yrs)
Talent Leads Consultants
RS 2,00,000 To 4,50,000 Mumbai, Mumbai Suburb
Employer Branding / Campus Recruitment - HR Projects - Lead delivery of end to end Recruiting service across all hiring levels, including branding, sourcing, and contracting - Manage resource allocation within the recruitment team - M...
Key Skills : hr manager,hr,hr recruiter,hr executive
Executive- Purchase ( 0 - 2 yrs)
RS 1,25,000 To 1,50,000 Goregaon, Mumbai
Position: Executive- Purchase & Admin Qualification: Graduate Experience: 0.5 to 2 Years Salary: 9 k-11 k Location: Goregaon Department: Procurement & Admin- Report to HR Manager & GM Procurement Job Description: 1. Adapt at managing admini...
Key Skills : executive- purchase,purchase executive,purchase officer,purchase head,procurement specialist,purchase coordinator,purchase manager,procurement...
Senior Manager - Recruitment - Call Center ( 10 - 15 yrs)
Talent Leads Consultants
RS 2,00,000 To 3,25,000 Mumbai
Leading a recruitment team for Talent Acquisition for call centers - Employer Branding / Campus Recruitment - HR Projects - Lead delivery of end to end Recruiting service across all hiring levels, including branding, sourcing, and contrac...
Key Skills : call center manager,marketing manager,business development manager
urgently require HR Generalist ( 2 - 8 yrs)
Prism Manpower Services
RS 2,00,000 To 4,75,000 Mumbai, Mumbai Suburb, Navi Mumbai
1) To handle all General ADMIN / HR Activities. 2) Recruitment , 3) Issuing Offer letter, 4) Maintaining all organization Documents. 5) Staff Retention, 6) Training, 7) Attendance, 8) Grievances, 9) Salary & Incentive Management, 10) ...
Key Skills : hr specialist,hr generalist,hr assistant,hr coordinator,hr executive,hr head,hr manager,assistant hr manager,hr officer,human resource management,hr...
Hr Payroll Specialist ( 1 - 4 yrs)
Ajit Sweets & Farsan
RS 1,25,000 To 2,25,000 Mumbai
Good At Payrolls , Musters Handling Paymnets and Fnf Settlement
Key Skills : payroll executive,salary administration,settlements employee,employee relations manager,settlements employee
opening for IT Engineer ( 0 - 1 yrs)
Pc Technology Pvt Ltd
RS 1,00,000 To 2,00,000 Mumbai
Job Description:- We are hiring for PHP, JAVA, .NET and Testing platform. You can select any one platform (Only one) among these platforms. The platform which you are applying for make sure you will have basic knowledge of it. SELECTION PROCESS: ...
Key Skills : java,php,.net,software testing engineer,it engineer,j2ee,struts,hibernate,advance java,spring,java beans,javafx,jface,enterprise...
Glenmark Pharmaceuticals Ltd
RS 2,25,000 To 4,75,000 Mumbai
Recruitment of Field Staff (FSO, DSM, ZSM & SM) - Partnering with the business to identify talent - internally and externally for field sales force across levels. To maintain 100% manning within TAT. - Attrition Control - Establishing touch poin...
Key Skills : hr manager,hr officer,hr specialist,hr analyst
Career and Technical Education India
RS 5,50,000 To 12,00,000 Mumbai
(1) Influencing and facilitating the process of organizational change and development, leading to improved organizational and individual performance. (2) Facilitating and providing technical inputs to the process of change management in cooperation ...
Key Skills : hr head,hr officer,hr recruiter,hr specialist
Priya It
RS 1,00,000 To 1,75,000 Pune
Hello Candidates, We are looking for HR cum Admin for Pashan Baner Location Eligibility: . • Smart, intelligent, and should be able to communicate in English and local language. Job Description • To maintain and pay bills online. •...
Key Skills : hr executive,hr assistant,hr associate,hr generalist,hr recruiter,hr specialist
ERP System Adminstrator ( 1 yrs)
Yodot Services
RS 50,000 To 2,00,000 Nagpur
Perform/Direct applications programming for ERP, HR, Payroll, analytics, intranet, public web site and other enterprise application systems. - Develop vision and roadmap for managing and assessing Enterprise applications. - Establish and oversee c...
Key Skills : erp,erp system administrator,erp executive,erp developer
Require Hr Recruiter in Consultancy ( 0 - 1 yrs)
Suggest Job Consulting Services
RS 70,000 To 1,25,000 Pune
- Reaching the Defined Targets for Hiring of Different Job Families (time, Costs, Quality) - Full Execution of the Recruitment Process (including Job Posting, Phone Interviewing, Job Interviewing, Regular Communication with Candidates and Managers) ...
Key Skills : hr,communication,administration
Recruiter or Hr Executive ( 1 yrs)
Pryme Placement
RS 1,00,000 To 1,50,000 Navi Mumbai
sourcing through job portals, networking. · Short listing resumes and initial screening of candidates. · setting up meetings/interviews. · making calls and sending mails. · handling queries. · Updating and maintaining database. ...
Key Skills : hr executive,hr assistant,hr associate,hr generalist,hr recruiter,hr specialist
Sr HR Manager - Strategic Talent Acquisition ( 4 - 6 yrs)
Talent Leads Consultants
RS 7,00,000 To 9,00,000 Mumbai
Strategic Talent Acquisition role - Employer Branding / Campus Recruitment - HR Projects - Lead delivery of end to end Recruiting service across all hiring levels, including branding, sourcing, and contracting - Manage resource alloca...
Key Skills : hr manager,hr,hr recruiter,human resource manager,human resource officer,placement manager,employee recruiter
Pc Technology Pvt. Ltd
RS 1,00,000 To 1,75,000 Thane
Greetings from PC Technology Pvt Ltd!!!!! Walk In & take the offer. We are hiring software freshers for the following platforms: • JAVA, • .Net, • PHP, • TESTING Educational Criteria: BCS , BCA, MCA, BE BTECH (IT,COMPUTERS and ExT...
Key Skills : it engineer,java,.net,php,software testing enigneer,pmis specialist,ict application...
Rajab Manpower Consultants
POST TITLE: Senior HR Executive EXPERIENCE: 4 to 5 years (in industry like salon, spa, wellness etc.) SALARY: UPTO 20-25K p/m QUALIFICATION: ANY GRADUATE/MBA/Diploma REPORTING TO: Assistant HR Manager DUTIES AND RESPONSIBILITIES: - Hard core r...
Key Skills : hr supervisor,senior hr executive,hr analyst,hr associate,hr coordinator,hr specialist
Hiring for Fresh IT Freshers ( 0 - 1 yrs)
Pc Technology Pvt. Ltd
RS 1,00,000 To 2,00,000 Thane
Greetings from Pc Technology Pvt Ltd!!!!! this is Ashwini from Pc Technology Pvt. Ltd. Its a Excellent Career Development Opportunity for Fresh It Graduates to Learn the Latest Cutting Edge Technologies On Live Projects. Candidate Should have Abilit...
Key Skills : java,it engineer,java developer,software developer,j2ee,struts,hibernate,advance...


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I am continuing with my year-end wrap-up posts for 2015. Today is part one in a two-part series debriefing my "Love it, Wear It" Challenge (LIWI), which I took on throughout all of last year. Part one will cover my clothes and part two, which I will publish early next week, will look at my shoes and accessories. In addition to writing about how the challenge impacted my wardrobe and style, I will also share an inventory of my wardrobe in these two posts. An Overview of LIWI But before I … [Read more...]
Archives for January 2016
"Love It, Wear It" Challenge Debrief & Year-End Inventory: The Clothes
January 2016 "Grab Bag" of Useful Links
We're almost at the end of the first month of 2016, believe it or not. I still have few more 2015 wrap-up posts to go, including a recap of my "Love It, Wear It" Challenge and a look at how I did with my purchases from last year. But before I publish those highly statistics-laden posts, I want to take a time out to share my latest "grab bag" of useful links (see previous editions here). Included below are links to articles I think you'll enjoy on the subjects of shopping and shopping … [Read more...]
2015 By the Numbers: Wardrobe Benchwarmers and More Statistics
Earlier this week, I shared the good news portion of my 2015 wardrobe review, my closet "all-stars." I appreciate all of the wonderful and detailed comments I received on that post. Some of you asked such great questions and made such insightful points that I now have several ideas for future posts. I really do get some of my best post ideas from readers. Before I delve into the flip side of my wardrobe numbers for last year, the "benchwarmers," I would like to share some more statistics … [Read more...]
2015 By the Numbers: The Wardrobe "All-Stars"
I am devoting a large proportion of my January posts to wrapping up last year. It's now time to take a look at some more statistics, this time my wardrobe "all-stars" for 2015. I have now been tracking my wardrobe for five years! Ever since the beginning of 2011, I've recorded how often I've worn the clothing and shoes in my closet. I mostly only track "out and about" clothing, but I've started tracking at least some of my at-home wardrobe as well. There is now more crossover between the … [Read more...]
A Pragmatic, Multi-Phased Approach to a Workable Wardrobe
The following is a guest post from Barb, who agreed to share her "story of recovery" with all of you. Barb is an active member of the "End Closet Chaos" closed Facebook group. She shared a big part of her story about downsizing her wardrobe and upping her style quotient via a multi-phase system over the past two-plus years. I thought Barb's pragmatic approach would be both educational and inspiring for readers of "Recovering Shopaholic," so I asked if she would be willing to share her story … [Read more...]
December Accountability Update and 2015 Wrap-Up
It's time for my final accountability update for 2015. As long-time readers know, these monthly reports are where I share what came into and left my closet during the previous month, what I wore, and how I did with my shopping budget. Sharing what I bought, what I culled, and how much I spent helps me to stay honest and on track with my wardrobe and shopping. This update will also include a year-end wrap-up in terms of my budget and in and out item totals. Other statistics – including … [Read more...]
Finding Balance in the Closet and All Areas of Life
For the past two years and at various points in the past, I have chosen a word or theme to guide my focus over the course of the year. For 2014, I actually selected two words, simplicity and joy, but I felt that diluted my focus a bit, so I returned to one single word, deliberate, to guide me during 2015. I've opted to continue the practice of choosing a word for the year for 2016. This post will be centered around sharing that word, why I have selected it, and how I see my theme unfolding … [Read more...]

subset: commoncrawl


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Looking for a Used Sequoia in your area?
CarGurus has 5,986 nationwide Sequoia listings starting at $1,495.
2005 Toyota Sequoia Overview
The 2005 Toyota Sequoia got a makeover for 2005, with a new grille and taillights among the cosmetic changes. The engine also got a boost in power, and the Limited has the option of second row bucket seats instead of a bench, which reduces the seating to seven people.
The 2005 Sequoia is available in two trims, the SR5 and the Limited. The Limited is the more luxurious version, with leather seating, front and rear air conditioning, and an upgraded stereo. Both share a 4.7-liter, 282 power V8, and both are available as a two or four-wheel drive. Not surprisingly, fuel economy is 15/18.
Drivers like the 2005 Sequoia because it handles well despite its size, offers plenty of cargo room and driver comfort, and handles well in the snow. One significant complaint is that there is limited visibility in the rear.
What's your take on the 2005 Toyota Sequoia?
2005 Toyota Sequoia Top Comparisons
Users ranked 2005 Toyota Sequoia against other cars which they drove/owned. Each ranking was based on 9 categories. Here is the summary of top rankings.
Cars compared to 2005 Toyota SequoiaPeople researching a 2005 Toyota Sequoia contacted a dealer about
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Toyota Sequoia Questions
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I bought a Toyota Sequoia from Nice Ride Auto. The people there were nice and the price was good.
2005 To Get A Score
My 4wd drive button was pushed accidentally on my 2005 Toyota Sequoia after that it will not drive or take reverse what could it be
Oil Type
What type oil does a 05 toyota sequia sr5 2wd 4dr v8 use
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Trouble starting.
The temp gauge on my 2005 Sequoia would rise as I sat at a stop light but would level out once I started moving down the road. I checked my coolant reservoir and it was just below "L". So I just stopp...
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Toyota Sequoia Experts
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Twenty-five years after Italia '90, Lady Robson reflects on her late husband's legacy
She has been a regular at St James' Park this season, just as her husband would have been. She had been present, too, for Sunderland's final home game, up in the stands, watching and feeling, maintaining a tradition in a manner that still surprises her. It is a link to her past, to Sir Bobby Robson and his great obsession, but after all this time, football has burrowed deep beneath her skin.
"I really didn't think it would play such a part in my life any longer," Lady Elsie Robson said. "But I suppose when you've lived with something so


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NYE on Carnival Valor: Carnival Valor Cruise Review by Airbubble
Overall Member Rating
NYE on Carnival Valor
Destination: Western Caribbean
Embarkation: Miami
New Year's Eve was amazing with the party on the lido and free flowing champagne with Times Square countdown on the big screen.
Dunns falls excursion with the carnival was only $20 different to a local company so easier and safer to book with the ship.
Getting to Washington dining room is a bit complicated as the ships galley is located in the centre of the ship so it's down to deck one or two walk to the aft then use them elevators to go up to deck three or four,you can't get there just walking along deck three,four or five.
Cozumel I booked a driver for the More day $90 for 3 hours then $20 an hour after that,he can take up to 8 people, he is crescencio Lopez Tun,email email@example.com or search him on Facebook he is the best and was recommended to me.
Kids clubs were great never saw the kids the whole two weeks when on sea days or evenings.
Staff were friendly and always called you by your name,on your time dining you can request where to sit so you can keep the same waiters if you wish.
I would use carnival again but maybe try a newer ship. Less
Published 01/18/13
Cabin review: 6A1240 Ocean View
Cabin 1240 in a very quiet location, nice size window, could do with more closet hangers, plenty of room with 2 adults and 2 kids in it. Tv needs updating. But overall not a bad cabin if wanting quiet time.Read All Ocean View (6A) Reviews >>
Port and Shore Excursions
Stingray Swim
Beach Break
Cobaya Gardens
Dunn's River Falls
Read Cruise Critic's Carnival Valor Review >>
Great 5 Night Cruise on the Ca...
It was ok, not our best cruise...
Avoid Promenade view stateroom...
Easy living
GREAT Experience! Loved this c...
Good value for a short cruise
Great 5 Night Cruise on the Ca...
Worst cruise EVER
It was ok, not our best cruise...
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text: Several election integrity activists have alleged that the Patient Protection and Affordable Care Act (PPACA), or Obamacare, does not appear to have been an effort to conduct healthcare reform to fix what was described in 2009 and 2010 as a broken healthcare system. The law passed Congress with just Democratic Party support. Instead of being an honest attempt to fix America's healthcare woes, "I think [it] is the biggest voter registration fraud scheme in the history of the world," said Gregg Phillips, the founder of the election integrity group Voters Trust, in an interview with Breitbart News Wednesday morning. Phillips and Catherine Engelbrecht of True The Vote, another election integrity group, cite as proof what left-wing organizations are saying about how they plan to use Obamacare. Demos, a left-wing organization funded by liberal billionaire George Soros, published a report earlier this year titled: "Building a Healthy Democracy: Registering 68 Million People to Vote Through Health Benefit Exchanges." http://www.breitbart.com/Big-Govern...st-Voter-Registration-Fraud-Scheme-in-History


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A very nice all day vape that is fruity but also has a nice cream taste to it. I make it at 70 vg 30 pg at 3% nicotine. Only problem is I can't get enough of it I just love the taste.
A very nice all day vape that is fruity but also has a nice cream taste to it. I make it at 70 vg 30 pg at 3% nicotine. Only problem is I can't get enough of it I just love the taste.
Using the flavour percentages how much liquid will this make.
SteveAll depends how much liquid would you like to make.
do you have to diluting when making your own ejuice sorry if this is a stupid question but i'm new to all of this thanks..
David, Your question is not stupid at all. Some people do use deionised water to dilute the e juice but is not something we recommend when mixing.
Hello every one maybe some one have or can tell me the Caribbean twist recipe? or ingredients? Long time i smoke this flavor and now i want to make it my self.
Hi ErnestasWe do have an unnamed Caribbean like recipe that we will be uploading onto our recipe page soon. The flavours include:8% Peachy Mix TFCC 5% Pineapple TFA 2% Mango Alfonso TFCC 1% Ethyl Maltol FWLeave to steep for 4-5 days.Thank you Jamie
I mixed this at 3mg, 70:30. Tried after a 2 week steep. It is a very nice fruity vape with a cream finish. Reminds me a little of Squid Ink by Kraken which I used to love. Well worth a try.
the 500 ml kits do i have to buy nicotine for it or is it premixed, if not what size bottle nicotine would i need for the 500ml kits, i am new to making my own so be 1st time also i like a description of the mixing accessories that come with the kit a better photo of them
also i plan to make single flavours juice what would you recomend to start of with, also a tobbacco flavour
Hi Harry,Our XL DIY E-liquid mixing kit include: 1 x 500ml - PG or VG 1 x 500ml - PG or VG 6 x 10ml - Flavour Concentrates 1 x Large - Mixing Accessory Kit Nicotine is not include in our kits, but you can add nicotine to your order. We have nicotine in a bottles of 100 ml and 50 ml, in two different strengths - 50 mg and 72 mg, and in VG or PG bases. I would suggest to start mixing smaller batches like 10 or 20 ml and maybe adding different flavour concentrates to try what you like. Have a look at our video guides on blog and YouTube, E-liquid recipes and E-liquid calculator. If you will have any questions, just give us a call!
My wife loves this, says it tastes like love heart sweets!
Hi Has anyone got a recipe for Cornish ice cream
Loving the very berry very smooth and creamy
Hi. I want to make this recipe but I'm confused by the fact that it says black cherry twice ?
Daniel, Thanks for pointing that out. It looks like a mistake in the site migration. The second one should be Raspberry TFA. I have now updated it. Thanks, James.
Lovely ... I've made a 50/50 mix with 12mg nicotine... it's awesome
hi, I want to make 100 ml 30pg/70vg.Is it still just 18% flavour?
Yes. It will always be 18%, but obviously the ml amount you have to add will change depend on amount of e-liquid you want to make.


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Class Notes
Class Notes | Obituaries
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Alicia Giovannini '96 and her husband, Andre Benguerel, welcomed their second son, Enzo Augustus Benguerel, on Dec. 10, 2013. The family lives in San Francisco, where Alicia works as a Realtor.
Lauren Gallagher '96 and husband Don Reierson welcomed their second child, Fiona Jane Reierson, June 28, 2012. She joins their 2-year-old daughter Adelyn Mae. The family lives in Los Gatos, Calif. Lauren is controller at Humane Society Silicon Valley.
Máire Ford '96 is an assisant professor of psychology at Loyola Marymount University.
Dr. Shana Fogarty '96 writes, "My husband and I just adopted our second son (Carter) in January 2015. We adopted our older son (Matthew) in February 2009. I work as a hospitalist and hospice medical director. We're celebrating our 10th anniversary next week. We've enjoyed the last 11 years in the Coeur d'Alene, Idaho, community."
Allan Evans MBA '96 has been appointed CEO of Samplify Systems Inc., provider of intellectual property for computing, consumer, and mobile device markets. Evans will lead Samplify to the next level in its corporate evolution by focusing the company, and its next-generation APAX(TM) application acceleration technology, on multibillion dollar markets including high-performance and cloud computing, Big Data, consumer electronics, and mobile devices.
Greg Czarkowski J.D. '99 and Susan Garci '98, M.A. '02 welcomed their second child, Xavier Francisco Garcia-Czarkowski on March 23, 2007.
Evangeline "Vangie" Maynard Cumming '96, MBA '13 married Stephen Ballard Cumming in a beautiful ceremony in Palos Verdes, Calif. Alumni in attendance included her brothers, Paul Maynard '89 and Ryan Maynard '91, and friends Valeri Yee '96, Christy Duncan-Anderson '96, Dawn McIntosh M.S. '10, and Sonya Duffin '98.
Michael Cole '96 and wife Teresa welcomed daughter Sienna on July 13, 2008. Michael is a pharmaceutical sales representative for Takeda Pharmaceuticals in San Diego.
Michael Cole '96 and wife Teresa welcomed their son, Cameron, on Nov. 3, 2011. Cameron joins his sister Sienna, age 4. They live in San Diego.
Kenton D. Chow '86, MBA '96 has joined Wine.com, the nation's No. 1 online wine retailer, as Chief Financial Officer. In this newly created position, Chow will oversee the finance functions, business development, mergers and acquisitions and investor relations. Chow brings more than 25 years of experience in high growth public and privately held companies. Prior to joining Wine.com, he was the Chief Financial Officer at a number of high technology companies in Silicon Valley, including Aurora Networks, Catena Networks and Cobalt Networks. His experience includes leading a successful IPO, mergers and acquisitions, including Sun Microsystems' $2 billion acquisition of Cobalt Networks, and over $350 million raised in multiple equity and debt financings. He also held senior financial management positions at Sun Microsystems, Symantec, Bay Networks and PriceWaterhouseCoopers. (Chow holds a M.B.A. and B.S. in Finance from Santa Clara University. He is a certified public accountant in California and also served on the Advisory Board for the Leavey School of Business at Santa Clara University.
Kenton Chow '86, MBA '96 is now the Chief Operating Officer of RetailNext, Inc., the leading big data analytics software company for physical retail stores worldwide. He and his wife of 19 years, Teri, live in Almaden Valley in San Jose with their two daughters, Kendra 17, and Kianna 14.
After spending the last 20 years as a CFO/COO for Silicon Valley based venture backed companies, Kenton Chow '86, MBA '96 joined as a partner with Financial Leadership Group (FLG) Partners (www.flgpartners.com), the premier CFO service firm in the San Francisco Bay Area. He and his wife, Teri, live in Almaden Valley in San Jose, and have three daughters: Mikaela (Babson College), Kendra (University of Virginia), and Kianna (Archbishop Mitty High School).
firstname.lastname@example.com www.flgpartners.com
Dennis Chiu J.D. '96, an attorney and community leader in Santa Clara County, is a candidate for the El Camino Hospital District Board. He is running on a reform platform that emphasizes transparency after the purchase of the Los Gatos hospital, a critical grand jury report in 2011, and a LAFCo service review and audit that raised concerns. Read at interview with him at losaltos.patch.com.
Shauna L. Chastain '91, J.D. '96 is President of the Solano County Bar Association, and Bay Area Chapter Director of the Association of Certified Family Law Specialists.
Coby B. Carlson '96 and Marlee A. (Hubbs) Carlson '96 welcomed a son, Cormac Sullivan, on March 27, 2010. He joins big brother Cian, 8, and big sister Maeve, 5, at the family home in Madison, Wisc.
Shelyna Brown J.D. '96 was appointed as a judge to the South Bay's 79-member bench by California Gov. Jerry Brown.
Shelyna Brown, 41, has been a public defender in San Jose since 1997. Like the governor, she is a Democrat. A graduate of Santa Clara University law school, Brown takes a seat vacated by the retirement of Judge Eugene Hyman. She will be one of four African-American judges on the local bench.
Sam Bhaumik MBA '96 joined Square 1 Bank, a diversified financial services company, as an executive vice president and head of Silicon Valley. Bhaumik, who brings more than 25 years of debt financing and investment experience, will be a member of the senior leadership team. Prior to joining Square 1 Bank, he was senior managing director with Hercules Technology Growth Capital. He also spent two years as the New York Stock Exchange's vice president of the western region and was co-founder of the emerging growth division at Imperial Bank (which was later acquired by Comerica). Bhaumik also previously served as a vice president with Silicon Valley Bank's Technology Group.
Mike Betz and Alisa (Harmon) Betz '97 welcomed their second child and future bronco, Henry Michael Betz, February 5, 2009. Hank and older sister Alice look forward to meeting all other future SCU students at their parents' 15 year reunions (if not sooner).
Mike Betz '96 and Alisa (Harmon) Betz '97 are pleased to announce the birth of their third (and final) future Bronco, Charles Donald Betz on Oct. 14, 2012. Charlie joins his siblings Alice and Henry and has the distinct privilege of being the proud owner of numerous articles of hand-me-down SCU gear! Here's to hoping they all get in to SCU! Third one's free, right?
David Bernhardt '96 and wife Dorea welcomed their first child and daughter, Sierra Riley, on April 26, 2010. The family lives in San Jose.


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Make a style statement at Abu Dhabi's first fashion week
Yas Mall in association with Pink Caravan and Burjeel Hospital is running a series of activities from 15-18 October.
Celebrate Eid Al Adha with your family and loved ones at Yas Mall. Enjoy various activities designed for the whole family.
Yas Mall celebrates the 1st Emirati Women's Day. Join us on August 28th from 5 pm – 8 pm at Town Square to experience this memorable event and participate in exciting activities.
Yas Mall presents in association with the Royal Thai Embassy, the Thai Festival at Town Square
Join us for summer fun at Yas Mall. Enjoy exciting promotions and great entertainment. Shop for a minimum of AED 300 at any of the outlets to qualify.
Celebrate this EID with your family and loved ones at Yas Mall. Enjoy various activities designed for the whole family.
Date: June 26, June 27, July 3 and July 4, 2015
Experience the holy month of Ramadan at Yas Mall where the spirit of giving surrounds you. Embrace heritage and culture through variety of activities. Enjoy wide array of delicious cuisines, shopping and exciting family entertainment.
This Friday, two-time UEFA Champions League winner Marcel Desailly is appearing at the UEFA Champions League Experience. Come down at 4pm to see a legend.


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City Seasons Al Hamra reopens after extensive renovation
Published Date
UAE, January 2012 - The City Seasons Al Hamra Hotel in Abu Dhabi has reopened following an extensive refurbishment of its rooms and public areas. The new look rooms feature a palette of warm oranges, cool greens and elegant dove grays with comfortable coordinating carpets and curtains.
The hotel's main entrance and lobby area has been completely remodelled with the addition of a gift shop as well as a new business centre. Café Trottoir, the coffee shop located on the ground floor, has been designed to look out onto the street making it accessible for passers-by.
Also completely reconfigured and remodelled the hotel's all-day dining outlet, the New Season Restaurant has been given a Mediterranean style feel and serves a buffet style breakfast and lunch as well as an a la carte option for dinner.
There has been a significant investment in the health club facilities with the creation of a bigger gym area and the addition of a sauna, steam rooms, massage facilities and beauty salon. The gym area features the latest fitness equipment and aerobic machines.
"Our plan was to not only give the hotel a facelift but also to add more facilities for guests and we have achieved that in less than a year" said Mohamed Al Aamri managing director and owner representative of City Seasons Group of Hotels "The extensive renovations, which were designed to enhance rather than change the character of the hotel, touched upon every aspect of the property to make the best of the product"
As part of the refurbishment, the City Seasons Al Hamra Hotel has also added a new Thai restaurant which is set to open its doors next month. Located in the lobby the Keway-mai restaurant will serve a wide range of Asian fusion dishes and authentic Thai food.
The final touches are being added to the banqueting and conference facilities and to the club floor which now has a brand new lounge. These are set to be completed next month.
The City Seasons Al Hamra Hotel is located on Electra Street, in the heart of the bustling capital, a short distance from the popular Abu Dhabi Mall, business and government districts, 15 minutes away from the Abu Dhabi National Exhibition Center and 20 minutes from Abu Dhabi International Airport.
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Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.
Citation: Yoshimura Y, Zaima N, Moriyama T, Kawamura Y (2012) Different Localization Patterns of Anthocyanin Species in the Pericarp of Black Rice Revealed by Imaging Mass Spectrometry. PLoS ONE 7(2): e31285. doi:10.1371/journal.pone.0031285
Editor: Gustavo Bonaventure, Max Planck Institute for Chemical Ecology, Germany
Received: October 7, 2011; Accepted: January 5, 2012; Published: February 17, 2012
Copyright: © 2012 Yoshimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Program for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry (BRAIN) to NZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Rice (Oryza sativa L.) is one of the most important cereal crops in the world, especially in Asian countries. One of the various rice cultivars is black rice, which contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. The crude anthocyanin-rich extracts of black rice bran improve serum triglyceride levels, which contributes to the suppression of atherosclerosis [1], [2], and protects against insulin resistance [3], alcoholic liver injury [4], and light-induced retinal damage [5], [6]. Due to these human health benefits, anthocyanin compositions have been investigated not only in black rice but also in various plants, such as berries [7], black soybeans [8], [9], [10], and dark-colored grapes [11], [12]. Several studies of the molecular species of anthocyanins in black rice have identified cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, peonidin-3-O-glucoside, and cyanidin-3-O-rutinoside [13], [14], [15], [16]. However, there are virtually no localization studies of individual anthocyanin species in plant tissue because methodologies for investigating the localization, such as determining specific antibodies to anthocyanin, have not yet been developed. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an emerging technology that allows the simultaneous investigation of the content and spatial distribution of a wide range of biomolecules such as lipids [17], [18], [19], glycolipids [20], [21], amino acids [22], proteins [23], and peptides [24], [25], as well as administered pharmaceuticals [26] without requiring antibodies, staining, or complicated pretreatment steps. Our previous study demonstrated that MALDI-IMS combined with the Kawamoto method, which uses adhesive films to prepare the cryosections, is an effective method for investigating the localization and composition of rice metabolites [27]. In this study, we investigated the localization of several anthocyanin species in black rice tissue in detail by MALDI-IMS and revealed the different localization patterns of anthocyanin species in the pericarp of black rice.
Identification of anthocyanins in the crude extract from black rice seed
Figure 1A is an image of a whole black rice seed used in this study. A 10-µm- thick cryosection shows that the black or purple pigment is localized in the pericarp and seed coat layers (Fig. 1B, C) and that the pericarp and aleurone layers were thicker in the dorsal side than in the ventral side (Fig. 1B). We used MALDI-MS to analyze the crude extract to identify the anthocyanins in the pigmented layers of black rice. As shown in Figure 1D, abundant anthocyanin signals at m/z 419, 433, 449, 463, 465, and 479 were observed in the mass spectrum. We analyzed precursor ions by tandem mass spectrometry (MS/MS) to identify the molecular species of anthocyanins in the crude extract. The MS/MS spectrum of the m/z 419 ion in the crude extract demonstrated an intense ion at m/z 287 (neutral loss of 132 Da), which would correspond to the loss of a pentose moiety (Fig. 2A). Unfortunately, the MALDI-MS system could not differentiate between molecular species of sugar moieties that have the same molecular mass, such as xylose and arabinose. The MS/MS fragmentation of the ion at m/z 287 would correspond to the cyanidin aglycone. Thus, we identified the m/z 419 ion as an [M]+ ion of cyanidin-3-O-pentoside (Fig. 2A). The MS/MS spectrum of the m/z 433 ion demonstrated an ion at m/z 271 (neutral loss of 162 Da), which would correspond to the loss of a hexose moiety (glucose or galactose), and the product ion at m/z 271 ion would correspond to the pelargonidin aglycone (Fig. 2B). Therefore, we identified the m/z 433 ion as an [M]+ ion of pelargonidin-3-O-hexoside (Fig. 2B). Similarly, because the ions in the black rice crude extract at m/z 449, 463, 465, and 479 also yielded the characteristic neutral loss of 162 Da corresponding to the loss of a hexose moiety, these ions were identified as the [M]+ ions of cyanidin-3-O-hexoside (Fig. 2C), peonidin-3-O-hexoside (Fig. 2D), delphinidin-3-O-hexoside, and petunidin-3-O-hexoside (Table 1), respectively. The molecular species identified in MS/MS analyses are summarized in Table 1. Recently, cyanidin-3-O-gentiobioside consisting of 2 glucose moieties and a cyanidin aglycone was identified in black rice [28]. We performed MS/MS analyses on the m/z 611 and 625 precursor ions to examine the possibility that this anthocyanin diglycoside might be included in the black rice crude extract used in this study. MS/MS analysis of the precursor ions at m/z 611 yielded an ion at m/z 287 corresponding to the aglycone cyanidin, which could be formed by a neutral loss of 2 hexoses (Fig. 2E). A small peak detected at m/z 449 indicated the possibility that the ions formed with the loss of a hexose moiety (Fig. 2E). To examine whether the neutral loss of 324 Da (m/z 611→287) corresponded to the loss of 2 hexoses, we performed further MS3 analysis on the product ions at m/z 449 formed from the ions at m/z 611. The ions at m/z 287 were detected and were shown to be formed from the ions at m/z 449 with a neutral loss of 162 Da, corresponding to 1 hexose (Fig. 2F). These results indicate that the ions at m/z 287 were formed from the ions at m/z 611 with a loss of 2 hexoses. Thus, we confirmed that the ions at m/z 611 in the black rice crude extract were [M]+ ions of cyanidin dihexoside.
A, Appearance of black rice. B, Black rice section stained with toluidine blue O. Scale bar: 1.0 mm. C, Enlarged image of the dorsal region of the longitudinal black rice section. D, Mass spectrum of the black rice crude extract.
A–D, [M]+ ions at m/z 419 (A), m/z 433 (B), m/z 449 (C), and m/z 463 (D). Neutral losses (NL) of 132 Da and 162 Da indicate losses of a pentose and hexose, respectively. E, F, [M]+ ions at m/z 611 (E) and MS3 spectrum of ions at m/z 449 derived from ions at m/z 611 (F).
Similarly, we identify [M]+ ions at m/z 625 as another anthocyanin dihexoside, which consisted of 2 hexoses and peonidin as an aglycone (Table 1).
IMS analysis of anthocyanins in black rice tissue
We analyzed black rice sections by MALDI-IMS to investigate whether anthocyanins can be visualized in black rice tissue. As expected from the localization of black pigment in the sections (Figs. 1B, 3A), ions at m/z 449 predicted as cyanidin-3-O-hexoside were distributed in the outer pericarp and seed coat layers (Fig. 3B), and were clearly separated from the nucellus epidermis/aleurone layer marked by phosphatidylcholine (PC; diacyl C36:3) (Fig. 3C), and the endosperm was marked by lysophosphatidylcholine (LPC; C16:0) (Fig. 3D). Similarly, ions at m/z 463 predicted as peonidin-3-O-hexoside also were distributed in the outer pericarp and seed coat layers (Fig. 3G). Merged images of anthocyanins, PC, and LPC are shown in Figure 3E and J.
A, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at m/z 449. B, Localization pattern of the ions at m/z 449. C, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3), which marks the nucellus epidermis/aleurone layer. D, Localization pattern of the ions at m/z 496 corresponding to LPC (C16:0), which marks the endosperm. E, Merged ion image of m/z 449 (red), m/z 822 (green) and m/z 496 (white). F, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at m/z 463. G, Localization pattern of the ions at m/z 463. H, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3). I, Localization pattern of the ions at m/z 496 corresponding to LPC (C16:0). J, Merged ion image of m/z 463 (red), m/z 822 (green) and m/z 496 (white). Scale bar: 1.0 mm.
IMS based on MS/MS analysis of anthocyanins on black rice tissue
To confirm the molecular species of anthocyanins in black rice tissue, we analyzed the black rice section with MALDI-IMS based on MS/MS (MALDI-IMS/MS) (Fig. 4A, B). Peaks corresponding to a neutral loss of 162 Da, hexose, were detected at each MS/MS spectrum. The product ions at m/z 287 (neutral loss of 162 Da) derived from the precursor ions at m/z 449 correspond to the cyanidin aglycone (Fig. 4A), and the product ions at m/z 301 derived from m/z 463 correspond to the peonidin aglycone (Fig. 4B). Although a single product ion at m/z 287 corresponding to a neutral loss of 162 Da was detected at m/z 449 in MS/MS analysis of the crude extract (Fig. 2C), other product ions at m/z 317 were detected in MS/MS analysis of the black rice section (Fig. 4A). The product ion at m/z 317 derived from the precursor ions at m/z 449 resulted from the neutral loss of 132 Da, pentose, and corresponds to the petunidin aglycone. Similarly, both m/z 301 and m/z 331 ions were detected from m/z 463 precursor ions in MS/MS analysis of the tissue section (Fig. 4B). The product ion at m/z 331 corresponds to the malvidin aglycone. These product ion images were used to investigate the distribution of each anthocyanin species in black rice tissue. Cyanidin-3-O-hexoside (m/z 449→287) was localized primarily in the dorsal posterior side of the pericarp (Fig. 4D, lower right corner), whereas petunidin-3-O-pentoside (m/z 449→317) was localized in the entire pericarp (Fig. 4E). Their distributions were different from the distribution of PC (diacyl C36:3), which was localized in the nucellus epidermis/aleurone layer (Fig. 4F). The merged image of these molecules is presented in Figure 4G. Similarly, peonidin-3-O-hexoside (m/z 463→301) was localized primarily in the dorsal posterior side of the pericarp (Fig. 4I, lower right corner), and malvidin-3-O-pentoside (m/z 463→331) was localized in the entire pericarp (Fig. 4J). We confirmed that their distributions were different from the distribution of PC (diacyl C36:3) (Fig. 4K, L). These results suggest that anthocyanins composed of pentose were distributed in the entire pericarp and anthocyanins composed of hexose were localized predominantly in the posterior dorsal side of the pericarp; in other words, the different localization patterns of anthocyanin species were due to different compositions of sugar moieties rather than to aglycones.
A, B, MS/MS spectrum of ions at m/z 449 (A) and m/z 463 (B) obtained from the pericarp of a black rice section. C, Optical image of a black rice section after analysis of the ions at m/z 449. D, E, Localization patterns of the fragment ions at m/z 287 (E) and 317 (F), derived from the parent ions at m/z 449, corresponding to cyanidin-3-O-hexoside and petunidin-3-O-pentoside, respectively. F, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3), which marks the nucellus epidermis/aleurone layer. G, Merged ion image of m/z 449→287 (red), m/z 449→317 (green) and m/z 822 (blue). H, Optical image of a black rice section after analysis of the ions at m/z 463. I, J, Localization patterns of the fragment ions at m/z 301 (I) and m/z 331 (J), derived from parent ions at m/z 463, corresponding to peonidin-3-O-hexoside and malvidin-3-O-pentoside, respectively. K, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3). L, Merged ion image of m/z 463→301 (red), m/z 463→331 (green), and m/z 822 (blue). Scale bar: 1.0 mm.
Inter pericarp distribution of anthocyanins
To test the hypothesis, we examined the localization of 2 anthocyanin species that had the same aglycone but a different sugar moiety. In this experiment, we compared the distributions of cyanidin-3-O-hexoside (m/z 449→287), cyanidin-3-O-pentoside (m/z 419→287), and petunidin-3-O-pentoside (m/z 449→317). As mentioned above, the product ions derived from the precursor ions of cyanidin-3-O-hexoside (m/z 449→287) were localized in the posterior dorsal side of the pericarp (Fig. 5B); the fragment ions derived from the precursor ions of cyanidin-3-O-pentoside (m/z 419→287) and petunidin-3-O-pentoside (m/z 449→317) were distributed in the entire pericarp (Fig. 5C, D). Their merged image is illustrated in Figure 5E. We analyzed a transverse section of black rice to confirm the localization pattern of these anthocyanins. Cyanidin-3-O-hexoside (m/z 449→287) was localized predominantly in the dorsal side (Fig. 5G); cyanidin-3-O-pentoside (m/z 419→287) was colocalized with petunidin-3-O-pentoside (m/z 449→317) over a broad region of the pericarp (Fig. 5H, I). Their merged image is depicted in Figure 5J. Next, we quantified the intensity of each fragment ion in 4 regions on longitudinal sections of black rice (Fig. 5K). The intensity of the fragment ions corresponding to cyanidin-3-O-hexoside (m/z 449→287) was significantly higher in the posterior dorsal region than in the other regions (p<0.01, Fig. 5L). The intensity of the fragment ions corresponding to cyanidin-3-O-pentoside (m/z 419→287) (Fig. 5M) and to petunidin-3-O-pentoside (m/z 449→317) (Fig. 5N) was higher in the anterior dorsal region than in the other regions. Quantification of each fragment ion revealed the different localization patterns of anthocyanins in the pericarp of black rice. To confirm the results of quantitative analysis of MALDI-IMS/MS, we quantitated the contents of anthocyanin of each seed parts by an independent quantitative method. The black rice seed was divided into 4 pieces as mentioned in Figure 5K. Then anthocyanins in each part of 30 seeds were extracted, followed by separation and detection by high performance liquid chromatography (HPLC). HPLC chromatogram of anthocyanins from whole black rice seeds is shown in Fig. 5O. According to the retention time with corresponding authentic standards, peaks 3 and 4 were identified as cyanidin-3-O-glucoside and peonidin-3-O-glucoside, respectively. Peaks 1 and 2 could not be identified by comparison with authentic standards. Further studies will be needed to identify molecular species of these compounds. Although the peaks corresponded to cyanidin-3-O-pentoside and petunidin-3-O-pentoside were not detected in our HPLC analysis, cyanidin-3-O-glucoside and peonidin-3-O-glucoside in each seed part were successfully identified and quantified (Fig. 5P, Q). The contents of anthocyanin species quantified by HPLC analysis were in good agreement with the results of quantitative analysis of MALDI-IMS/MS (Fig. 5L and data not shown). These results indicate that the quantification of ion intensity on MALDI-IMS/MS image reflects practically the contents of anthocyanin in black rice seeds. Although the biological consequences of different distribution patterns of anthocyanin molecular species in the pericarp of black rice seed are not clearly defined now, our data suggested that anthocyanin species composed of different sugar moieties have specifically-different biological functions in black rice seed.
A–E, Longitudinal section images. Scale bar: 1.0 mm. F–J, Transverse section images. Upper side in transverse sections is the dorsal side of the black rice seed. Scale bar: 0.5 mm. A, F, Optical images. B, G, Localization pattern of the fragment ions at m/z 287, derived from the precursor ions at m/z 449, corresponding to cyanidin-3-O-hexoside. C, H, Localization pattern of the fragment ions at m/z 287, derived from the precursor ions at m/z 419, corresponding to cyanidin-3-O-pentoside. D, I, Localization pattern of the fragment ions at m/z 317, derived from the precursor ions at m/z 449, corresponding to petunidin-3-O-pentoside. E, J, Merged ion image of m/z 449→287 (red), m/z 419→287 (green), and m/z 449→317 (blue). K, Four regions defined in the intensity quantification of each fragment ion. L–N, Relative intensity of the fragment ions corresponding to cyanidin-3-O-hexoside (L), cyanidin-3-O-pentoside (M), and petunidin-3-O-pentoside (N). AV: anterior ventral region, PV: posterior ventral region, PD: posterior dorsal region, AD: anterior dorsal region. Data was collected from 7 sections of 3 black rice seeds (means ± S.E.). Different denote significant differences among groups means from a Tukey-Kramer test (p<0.01). O, HPLC chromatogram of anthocyanins in the black rice crude extract. Peak 1 and 2, unknown; peak 3, cyanidin-3-O-glucoside; peak 4, peonidin-3-O-glucoside. P, Q, Contents of cyanidin-3-O-glucoside (P) and peonidin-3-O-glucoside (Q) in each part of black rice seeds determined by HPLC and expressed in nmol per mg of seed pieces ± S.E in triplicate.
Although the distribution and composition of anthocyanin species in various plants have been studied previously [29], information about the interstitial localization of anthocyanin species in plant tissue is limited. In this study, we revealed the different localization patterns of anthocyanin species in black rice and demonstrated the efficacy of MALDI-IMS analysis for the detection, identification, and mapping of the various anthocyanin species in plant tissue.
Anthocyanins, a group of reddish-purple flavonoids, are the primary pigments in red and black seeds, and are responsible for the attractive red, purple, and blue colors of many flowers, fruits, and vegetables. Among the many species of naturally occurring anthocyanins, cyanidin-3-O-glucoside is the most common in higher plants [29]. Cyanidin-3-O-glucoside has been recognized as a potent bioactive molecule that protects against TNF-α–induced endothelial dysfunction [30], ethanol neurotoxicity in the developing brain [31], and tumor promoter-induced carcinogenesis and tumor metastasis [32] because of its high antioxidative activity. In addition, cyanidin-3-O-glucoside improves triglyceride metabolism in the liver of diabetic KK-Ay mice by inhibiting mitochondrial glycerol-sn-3-phosphate acyltransferase 1 activity through the phosphorylation of protein kinase C zeta [33]. Previous studies on the identification and quantification of anthocyanins in black rice have shown cyanidin-3-O-glucoside as the primary anthocyanin species [13], [14], [15], [16]. The data from this study support these previous studies (Figs. 1D, 5O). MALDI-MS and MS/MS analysis of anthocyanin-rich extract from black rice identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides (Fig. 2, Table 1). MALDI-IMS and MS/MS analysis on tissue sections revealed the existence of an additional 2 species of anthocyanin pentoside in the pericarp of black rice (Fig. 4A, B). These results suggest that MALDI-IMS analysis of tissue sections is more effective for identifying anthocyanins than MALDI-MS analysis of black rice crude extracts using acidified methanol as a solvent. Acidified methanol is an effective solvent for extracting anthocyanins from various plants (blueberry, potato, red cabbage); the acid lowers pH levels and prevents the degradation of anthocyanins. However, because hydrochloric or formic acid becomes concentrated as the acidified methanol evaporates, anthocyanin monoglycosides are converted into unstable aglycones [29]. Therefore, the minor anthocyanins in the black rice crude extracts may be degraded into sugar and aglycones during the evaporation step, leading to the trace detection of 2 species of anthocyanin pentosides in the crude extracts by MALDI-MS analysis (Fig. 2C, D). Even in the crude extracts prepared without concentration step, the minor anthocyanins were not identical in both MALDI-MS/MS and HPLC analysis probably due to their low amounts or less stability in extracting solvents (Figs. 2C, 2D, 5O). As a preparation step such as extracting and concentrating the target molecules is not required, MALDI-IMS analysis of tissue sections is a more effective method for identifying and mapping anthocyanin molecular species in plant tissues.
In this study, we conducted a detailed investigation of the localization of anthocyanin species in black rice tissue. Analysis of black rice sections by MALDI-IMS/MS revealed the different localization patterns of anthocyanin species composed of different sugar moieties. From the longitudinal section of black rice, we determined that cyanidin-3-O-hexoside (Figs. 4D, 5B) and peonidin-3-O-hexoside (Fig. 4I) were localized in the dorsal posterior pericarp. Observations of the transverse section determined that cyanidin-3-O-hexoside was localized predominantly in the dorsal pericarp (Fig. 5G). In contrast, cyanidin-3-O-pentoside (Fig. 5C), petunidin-3-O-pentoside (Figs. 4E, 5D) and malvidin-3-O-pentoside (Fig. 4J) were localized over a broad region of the pericarp. Glycosylation of anthocyanidins by glycosyltransferases increases their chemical stability and aqueous solubility in vacuoles. UDP-glucose: flavonoid 3-O-glucosyltransferase (UF3GT) is a well-characterized glycosyltransferase that catalyzes the transfer of glucose from UDP-glucose to the 3-position of anthocyanidins to form the corresponding anthocyanins. The expression of the uf3gt gene is one of the most regulated steps in anthocyanin synthesis and is required for the accumulation of anthocyanins in some plants [34], [35], [36], [37], [38], [39]. The seeds of the Arabidopsis thaliana mutant, anthocyaninless1 (ANL1) have a reduced anthocyanin content, and the gene responsible for ANL1 encodes UF3GT [36]. The rice genome has 2 orthologues of uf3gt, Os06g0192100 and Os07g0148200. Although these 2 orthologues have not been well-characterized, the mechanism of uf3gt expression in A. thaliana has been characterized. In A. thaliana seedlings, uf3gt expression is strongly up-regulated by sucrose [40]. In addition, A. thaliana uf3gt expression is induced by the production of anthocyanin pigment 1 MYB transcription factor, which is up-regulated by light exposure [41] and sucrose [42]. Sunlight exposure increases anthocyanin production in black rice [43], and several MYB transcription factors were identified as being seed-specific and regulating anthocyanin biosynthesis [44]. In the developing caryopses of rice, assimilated carbon such as sucrose is transported through the vascular parenchyma and pigment strand in the dorsal region of the rice seed, and forms a concentration gradient between the dorsal and ventral parts of the pericarp [45]. Sunlight and sucrose are thought to coordinate to induce uf3gt expression in the dorsal pericarp, resulting in dorsal localization patterns of anthocyanin species composed of a hexose moiety. This study will contribute to the investigation of expression patterns of anthocyanin-related genes and a better understanding of the biological significance of anthocyanin species in black rice.
Materials and Methods
Adhesive film (Cryofilm type IIC [10]) was purchased from Leica Microsystems (Tokyo, Japan). Glass slides (Fisherbrand Superfrost Plus) were purchased from Thermo Fisher Scientific (San Jose, CA, USA). Methanol and distilled water were purchased from Nacalai Tesque (Kyoto, Japan). 2,5-Dihydroxybenzoic acid (DHB) and carboxymethyl cellulose (CMC) were obtained from Bruker Daltonics (Bremen, Germany) and Wako Pure Chemical Industries (Osaka, Japan), respectively. Cyanidin-3-O-glucoside and peonidin-3-O-glucoside were purchased from Fujicco (Hyogo, Japan) and Tokiwa phytochemical (Chiba, Japan), respectively. All chemicals used in this study were of the highest purity available. Black rice (Oryza sativa L.) seeds harvested in Hyogo, Japan, in 2010 (Fig. 1A) were purchased from a local market in Nara, Japan.
Anthocyanin extraction from black rice
Mortars and pestles were used to grind 20 g of black rice. 1 g of the ground rice samples was extracted in 10 mL of methanol containing 1% HCl (v/v) for 24 hours in the dark at 4°C. The crude extracts were filtered with Whatman No. 2 paper. For quantification of anthocyanins in black rice seed parts, a black rice seed was divided into 4 pieces defined as anterior ventral (AV), posterior ventral (PV), posterior dorsal (PD), and anterior dorsal (AD) by a fine surgical knife as mentioned in Fig. 5K. Weight of each part pieces of 30 black rice seeds were 147 mg (AV), 165 mg (PV), 159 mg (PD), and 153 mg (AD). Then, anthocyanins were extracted from each part pieces of 30 seeds in 300 µL of methanol containing 1% HCl (v/v) for 2 days at 4°C. The crude extracts from seed parts were obtained by centrifugation at 10,000 g for 1 minute to remove debris. These crude extracts were then subjected to HPLC.
Quantification of anthocyanins in black rice seeds by HPLC
HPLC analysis was performed using a liquid chromatography system (SCL-10A, Shimadzu, Kyoto, Japan) equipped with two pumps (LC-10A), a control system (SCL-10A), a diode array detector (SPD-20A), a CTA-20A column oven, and a column COSMOSIL 5C18-AR-II 4.6×250 mm i.d. 5 µm (Nacalai tesque, Kyoto, Japan). The mobile phase consisted of water∶formic acid (96∶4, v/v) (A phase) and methanol∶formic acid (96∶4, v/v) (B phase). Sample elution used the time gradient program: 0–0.01 min 10% B, 0.01–5.00 min 20% B; 5.01–50.00 min 50% B; 50.01–60.00 min 100% B. 10 µl of sample were injected. Quantification was performed at λ = 520 nm. All chromatographic tests were carried out at 37°C with a flow of 0.5 mL/min. Cyanidin-3-O-glucoside and peonidin-3-O-glucoside was quantified using a calibration curve of the corresponding standard compounds. All determinations were performed in triplicate.
MALDI-MS and MS/MS analysis of anthocyanins
A 5-µL aliquot of black rice crude extract was mixed with an equal volume of 50 mg/mL DHB in methanol/water (7∶3, v/v). The mixed sample had been deposited onto glass slide and dried before MALDI-MS analysis. MALDI-MS analysis was performed using a MALDI linear quadrupole ion trap mass spectrometer (MALDI LTQ-XL; Thermo Fisher Scientific) equipped with a 337-nm nitrogen laser at a repetition rate of 60 Hz in positive ion mode. The laser energy was set to 8 µJ, and the mass spectrometer was operated in automatic gain control (AGC) mode. The number of micro scan and sweep shot were set to 1. Ions with m/z values in the range of 200–550 were measured. For MS/MS analysis, the selected precursor ions and the product ions obtained by collision-induced dissociation were ejected from the ion trap and analyzed. The collision energy was set to 20% of the maximum available energy required to completely fragment the precursor ion formed from the peptide Met-Arg-Phe-Ala [46]. The laser energy was set to 8 µJ. The number of MSn micro scan and sweep shot were set to 1.
Preparation of black rice sections (Kawamoto method)
Black rice sections were prepared according to the Kawamoto method with slight modifications [27], [47]. Briefly, black rice seeds were freeze-embedded with 2% CMC at −80°C. The frozen black rice seeds were attached to adhesive film and sliced to 10-µm thickness using a cryostat (CM 1850; Leica Microsystems, Wetzler, Germany). Sections were attached to a glass slide with electrically conductive adhesive tapes.
Imaging mass spectrometry
MALDI-IMS analysis was performed using the LTQ-XL mass spectrometer as described above. Samples were prepared according to the method published previously [27]. Briefly, 50 mg/mL DHB in methanol/water (7∶3, v/v) was used as a matrix. The DHB matrix solution (500 µL) was sprayed uniformly over the black rice sections using an airbrush with a 0.2-mm nozzle (Procon Boy FWA Platinum; Mr. Hobby, Tokyo, Japan). Data were acquired with a 50-µm step size in positive ion mode. The laser energy was set to 30 µJ, and the mass spectrometer was operated in AGC mode. The number of micro scan and sweep shot were set to 1. Ions with m/z values in the range of 350–1000 were measured. ImageQuest software (Thermo Fisher Scientific) was used to create 2-dimensional ion -density maps, normalize peak intensity, and to adjust the color scale. For MS/MS analysis of the anthocyanins in the sections, the collision energy was set to 35% of the maximum available energy for the LTQ-XL, and the laser energy was set to 30 µJ. The number of MSn micro scan and sweep shot were set to 2 and 5, respectively. Following MS/MS analysis, the same section was analyzed by MS as mentioned above or by another MS/MS. After MALDI-IMS analysis, the sections were stained with toluidine blue O. Semi-quantitative analyses of the ion intensity in a part of black rice section were carried out using ImageQuest software. Data was collected from 7 sections of 3 black rice seeds. Multiple comparisons between groups were made by Tukey-Kramer test.
Author Contributions
Conceived and designed the experiments: YY NZ TM YK. Performed the experiments: YY. Analyzed the data: YY. Contributed reagents/materials/analysis tools: YY. Wrote the paper: YY NZ.
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I know its a late reply but YES you can hes only using it to control a relay at the end of the day.View Instructable »
There are a lot of fixes (and causes) for that particular error.Its an old fix but still valid for some people.For more up to date answers I would suggest a search from inside the http://forum.arduino.cc/ forum to get all the stk 500 answers in a more usable bundle of results.View Instructable »
You might want to update the article soon as codebender is closing down...You could try the Arduino CREATE as that lets you share code tooView Instructable »
Have also started to notice manufacturers moving away from steppers to regular DC motors more so in printers and scanners. These also need quite a bit of accuracy esp. the high dpi units so methinks they have found a way to use DC motors without losing the accuracy of a stepper.View Instructable »
Watch out for those breadboard PSU'sThey can go south pretty quick if you draw too much current for a lone period of time. I have 3 in the garbage so far as the regulators on them are not always the best. Better to have a more reliable supply or one that can handle the current. So I recommend check the specs before you buy. I moved to some of the power puck types you can buy and things are a lot better here.View Instructable »
How did you get your guage on your public channel.?I don't get the same screens that you show and can only display on a private channelView Instructable »
This was a good primer.Some things seem to have changed a little but it still works.Now I just need to play a little to see how far I can take it. I wanted something better than the Arduino MKR1000 that I have because its so unreliable requiring full restarts and such and most of the projects require a paid subscription to Azure. N
OK changed my mind I didn't understand that this is a PAY FOR USE app.Unlike Thingspeak orEasyIOT which are free.
OK changed my mind I didn't understand that this is a PAY FOR USE app.Unlike Thingspeak orEasyIOT which are free.View Instructable »
just curious.you call R1 30k and R2 10k yet the schem shows 9k and 3k ?There is quite a difference and I just wondered which one was correct before I attempt this one.I would like to use this with a 7 seg display for my boat battery (12V) and have already made a sketch for it but before I hook it up I want to be sure I am not going to fry anything.Also am going to use a 3.3v pro mini so thinking that I should swap out the 5v (Vout) for 3.3 ??View Instructable »


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Derma E, Age-Defying Hand Creme, 4 oz (113 g)
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- All Skin Types
- Natural Solution: Age Reverse
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Age Defying Hand Creme helps reverse the visible signs of aging, relieve dry skin and support nail and cuticle health.
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derma e natural skincare: solutions that work
Suggested Use
Apply Age-Defying Hand Creme to your hands, including nails and cuticles. Massage in thoroughly. Apply whenever skin is dry and thirsty.
Other Ingredients
Water (aqua), allantoin, glycerin, ethylhexyl palmitate, caprylic/capric triglyceride, cetyl alcohol, dimethicone, tocopheryl acetate (vitamin E), organic aloe barbadensis leaf extract* (.1%), olea europaea (olive) fruit oil, cucumis sativus (cucumber) fruit extract, organic camellia sinensis (green tea) leaf extract* (.1%), polysorbate, stearyl alcohol, cetearyl alcohol, glyceryl stearate, glyceryl stearate SE, cetearyl glucoside, carbomer, panthenol, methyl sulfonyl methane, phenoxyethanol, ethylhexylglycerin, sweet pea fragrance.
*Certified Organic by Quality Assurance International or USDA.
Very good hand cream
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Very good hand cream
This cream really helps smooth out the fine lines on the tops of my hands that have been forming from the aging processs. I wish I had found this in time for last winter when my other creams didn't keep moisturizing like this one does. It's a good idea to use at bed time so that it will have all night to work before you have to wash your hands again. I love how it is fairly non-greasy once it absorbs, and the fragrance is nice and clean-smelling; something like mango and flowers, but not too sweet or strong. It's a very good cream overall.
Great for dry hands
Posted by 5190714549484903729 on Feb 22, 2017
I keep this by the bed and love using it. It soaks in straight away.
Smells nice like a bubble gum but mild. Good absorption.
Posted by 5536654188703829287 on Feb 16, 2017
It smell nice like bubble gum but not that strong. The absorption is OK. It does moisturised your skin and stay moisturised for quite a long period. Slightly sticky but bearable. However, keep using daily. Don't skip. Else your hands feels dry again. Good to use in the cold office environment. I recommend it.
Posted by 5214173865143696831 on Feb 12, 2017
This is a great moisturising hand cream, perfect after washing dishes. Light berry scent which is not overtly sweet.
Posted by 5346577446004461777 on Dec 19, 2016
This is moisturizing, just great for my dry hands. Absorb fast too. If you have dry hands like mine, where the skin tends to peel around the fingernails, try this one. It works for me. And the price is acceptable too.
Derma E Age-Defying Hand Cream
Posted by 5723458311739742986 on Dec 01, 2016
Amazing - I can see a real difference in my forearms and hands in only two weeks. Well worth it! Recommend


text: Alegria Cocina Latina may be outside of your area. Please enter your delivery address to check availability Do you need any... Napkins & Utensils Continue to Checkout Alegria Cocina Latina Back to Menu Your order refresh Alegria Cocina Latina Your Shopping Cart is empty edit Delivery Address Please enter delivery address edit Delivery Time N/A* Add someone to this order Shrimp Al Ajillo Sautéed jumbo gulf shrimp with a garlic and tequila cayenne sauce and fresh tomatoes. Served with steamed white rice and sautéed spinach. x $20.00 Special Instructions: Label Item For: or Cancel


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Sold separately, metal flag stand presses easily into the ground; 15" x 40"H.
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From wacky to wonderful, our unique collections of outdoor and garden gifts sprinkle personality into the garden. Personalize any part of your yard with our one-of-a-kind windsocks, banners, markers and more. Check out our other Indoor Décor items while you're here.


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Salman Rushdie is Writing Novel With Trump-Like Villain
Salman Rushdie is working on a new novel for Vintage imprint Jonathan Cape, which will be published in September, 2017. The novel, called The Golden House, is a thriller which set in the current day. From the description of the novel, it seems clear that the satiric novel will take aim directly at President Trump. Read more... February 10, 2017
Javaka Steptoe Wins 2017 Caldecott Medal for Radiant Child: The Story of Young Artist Jean-Michel Basquiat
Javaka Steptoe has won the 2017 Caldecott Medal for Radiant Child: The Story of Young Artist Jean-Michel Basquiat (Little, Brown). The picture book, which he wrote and illustrated, is a vibrant biography of street artist Basquiat. The book also is the winner of the 2017 Coretta Scott King Illustrator Award Read more... February 2, 2017
Literary Agents Put Out an Open Call for Submissions From Muslim Writers
A group of literary agents has put out an open call for submissions from Muslim writers. The agents write in the open call, "We all agree that the current political climate demands a need for a greater presence of authors of Muslim heritage in the book marketplace. We are taking action to help make that happen." Read more... February 1, 2017
New York Times Dumps Comics and Mass Market Paperback Bestseller Lists
Publishers and authors are reeling from the news that The New York Times is dropping a number of its bestseller list as of February 5, 2017. On the chopping block are the bestseller lists for graphic novels, manga, mass market paperbacks, young adult ebooks and middle grade ebooks. Publisher's Weekly reports that the change is part of a larger restructuring of the books section of The Times. Read more... January 31, 2017
Kelly Barnhill Wins 2017 Newbery Medal for The Girl Who Drank the Moon
The 2017 Newbery Medal has been awarded to The Girl Who Drank the Moon by Kelly Barnhill. The book was published by Algonquin Young Readers, an imprint which was founded in 2013. This is the imprint's first Newbery award. The fantasy novel is about a girl who has an unusual upbringing: she is raised by a witch, a swamp monster and a Perfectly Tiny Dragon. Fox Animation has snapped up the film rights to the book. Read more... January 30, 2017
A French Translator Describes the Difficulty of Translating President Trump
Robert Zaretsky of the L.A. Review of Books interviewed translator Bérengère Viennot about the challenges and joys of translating the words of American presidents for a European audience. In particular she bemoans the difficulty translators are having turning President Donald Trump whose style is so different from President Obama's. Read more... January 29, 2017
Merriam-Webster Calls Out Kellyanne Conway for 'Alternative Facts'
President Trump's former campaign manager and current counselor Kellyanne Conway caused jaws to drop this morning when she used the term "alternative facts" to explain why White House press secretary Sean Spicer presented at least three deliberate falsehoods in his first press conference yesterday. Read more... January 22, 2017
Trump Nominee Monica Crowley Felled by Plagiarism Scandal
Monica Crowley, Donald Trump's nominee to be senior director of strategic communications for the National Security Council, has withdrawn from consideration for the post after a plaigiarism scandal. The New York Times reports that the conservative commentator and author withdrew after allegations that she plagiarized major sections of her 2012 book What the (Bleep) Just Happened? Read more... January 17, 2017
Damien Chazelle Wins Best Screenplay and Best Director at Golden Globes for La La Land
Damien Chazelle took home the Golden Globe awards for Best Screenplay and Best Director at the 2017 Golden Globes. His film La La Land starring Emma Stone and Ryan Gosling was the dominant film at the Globes this year. It won seven awards including Best Picture. Read more... January 8, 2017
Donald Trump Boots Biographer Off Golf Course
President-elect Donald Trump kicked one of his biographers off his golf course Friday in Florida. Harry Hurt, the author of The Lost Tycoon: The Many Lives of Donald J. Trump, was set to play golf at the Trump International Golf Club in Jupiter, Florida, with billionaire and conservative mega donor David Koch. Hunt's 1993 book is an unflattering biography of the new president elect. Read more... January 1, 2017
A Princess and an Author: Carrie Fisher Taken Too Soon
Fans are in mourning after the news that actress and bestselling author Carrie Fisher has died at the age of 60. She suffered a major heart attack while flying home from London to Los Angeles. Although she was given CPR on the plane and was taken immediately to the hospital upon landing, she never regained consciousness. Her daughter, Billie Catherine Lourd, had her publicist confirm the sad news. Read more... December 28, 2016
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Donald Trump is known for making typos on Twitter. He uses his phone to enter his tweets and does not always spell check. Today, the President-elect took to Twitter to attack China for confiscating a U.S. drone. Read more... December 17, 2016
James Patterson Donates $250,000 to Independent Booksellers
James Patterson, in cooperation with the American Booksellers Association, announced the names of the independent booksellers that will receive grants from his Holiday Bookstore Bonus Program. Patterson, who is passionate about literacy and reading, will give out $250,000 total divided between 149 independent booksellers. The individual amounts range between $1,000 and $5,000. Read more... December 15, 2016
Patti Smith Performed A Hard Rain's A Gonna Fall as Bob Dylan's Nobel Prize Acceptance Speech
1500 guests wearing black tie attended the ceremony at the Stockholm Concert Hall in Stockholm, Sweden to preset the 2016 Nobel Prizes. The Nobel Prize for Literature was awarded to singer songwriter Bob Dylan, who did not attend. Patti Smith accepted the award on behalf of Dylan. Read more... December 10, 2016
Author Emma Straub is Opening a Bookstore in Brooklyn
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Delaware is located in Delaware county and has a population of 25,243.
For Ohio residents, the average tuition for all Delaware personal training schools for the 2009 - 2010 school year was $34,570.00. For non-residents, the average tuition for Delaware personal training schools was $34,570.00.
Books and supplies for personal training schools in Delaware cost an average of $1,200.00. There is one personal training college in Delaware. In the 2008 - 2009 school year, reportedly 0 students completed personal training programs in Delaware.
|Average Salary
|Number Employed in City
|Athletic Trainers
|Fitness Trainers and Aerobics Instructors


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|Torsten Curdt <tcu...@dff.st>
|Re: [VOTE] Adding "Expires" headers to pipelines
|Sun, 26 May 2002 15:18:46 GMT
> > I always thought this would be a good thing. Have you implemented it for > > the compiled sitemap, for the treeprocessor or for both? > > Alas, I was too short on time to catch up both with TreeProcessor anc > compiled sitemap, so as of now this is available only on TreeProcessor. great > I might do my best to reconcile this change and port it to the compiled > sitemap too, but I don't expect this to be an easy task. Besides, I > think this is about time to discuss about this issue and put some rules. > I see these possible choices: aggreed > 1. choose one implementation (be it treeprocessor or compiled) and stick > to it; > 2. keep both, and let developers work with the one they prefer (with > possible out of synch changes); > 3. keep both, but refuse to release components that work only with one > sitemap engine. > > Personally I'd go for 1 and choose treeprocessor, unless there are > benchmarks stating that a compiled sitemap is definitely faster. 3 is > good to, but I'd avoid 2 as hell. I'd go for 1) , too. So maybe we should vote on deprecating the compiled sitemap then... -- Torsten --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, email: email@example.com
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