text:
I will do Mixed Local Business Directory Submission
About This Gig
!!!Before start order we will need to discuss work clarification, it's avoid to misunderstanding both of us and project cancellation!!!
Mixed Link Building Service
What we do:
1st step
Create one Local Directory link
Manual Submission
Company Logo, photo, contact number, Address and URL
2nd step
Spread local directory link to following medias
Top 10 Web 2.0 Property Creation
- Article provide by client
- PR5+, 40+ Trust flow, Do-follow and high domain authority sites
- 1 back link with multiple anchor text
- Niche related 2 banner and 2 content images
- Customized thematic sites
10 Social bookmarking
- PR3+ sites
- Different titles and description
- Google friendly sites
10 High quality social media profile submissions
- Complete business/personal profile creation
- PR3+ sites
Do manual submission only
100% White hat method
Article provide by client
Complete report with login details


text:
Windows HPC Server 2008R2 Monitoring and Management Part One
Download this episode
Kathy Palmer, Lead Program Manager of the HPC Team, presents the management and monitoring enhancements released with R2. The notion of personal supercomputing is a core operational paradigm that is rapidly evolving in the numerical computing ecosphere. Various visualization aids, such as heat maps, provide administrators with hierarchical and aggregate views that simplify utilization, health management, and troubleshooting.
Available formats for this video:
Actual format may change based on video formats available and browser capability.
More episodes in this series
Overview of Microsoft Technical Computing and HPC Server 2008R2
Related episodes
Windows Azure Cloud Services Role Lifecycle
Introduction to Windows Azure Worker Roles (Part 1)
Introduction to Windows Azure Worker Roles (Part 2)
Azure RemoteApp Hybrid Deployment overview
Azure RemoteApp Cloud Deployment overview
Windows Azure Cloud Services Concepts (Part 1)
Windows Azure Cloud Services Concepts (Part 2)
Azure Batch
Azure RemoteApp pricing overview
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subset: commoncrawl


text:
|Property type
|Minimum Stay
|3 nights
Welcome to the stunning vacation place. A beautiful property with 3 spacious bedrooms & 2 elegant bathrooms, a property that can accommodate comfortably up to 8 guests. With hardwood floors throughout, with granite counter tops, with a fully equipped kitchen, with high ceilings, with high speed Wi-Fi, with stainless steel appliances, with full washer & dryer, with a balcony, with 4 big screen TV's, with 4 total beds ( 2 queens & 2 fulls), with walking closets, with a superb pool & spa ( please note that pool & spa are open from April 15th until November 15th), a fully equipped gym, as well as a BBQ area. With all of these features, amenities and more you will feel amazing everyday of your stay & you will come visit us again!!!
The property was perfect for our needs. We had relatives nearby that we were visiting, and four of us stayed here and had our own, very comfortable space. It's pretty close to Red Rock Canyon, and we hiked there a couple of days. Trader Joe's and other stores are close, plus there's a nice park / trail a couple of blocks away in Summerlin.
perfectly clean and nice. good price and close to the strip Uber $ 15.
will rent again next time we come down.
My husband and I along with our two daughters rented this home for a week in December. It was really a great set-up for us. First, the home was very clean and comfortable with all the conveniences (a fully equipped kitchen, washer & dryer, WI-FI, and four flat screen TVs ). It is tastefully decorated and looks amazing. The grounds of the complex were nicely kept. The complex had a big pool and a spa. It is located very close to the Strip and the Buffalo shopping plaza. This home was a very nice place to stay and we wouldn't hesitate to stay there again. The owner was also very pleasant!
The townhome/condo worked out really great for us. The unit is in a gated community and is close to grocery shopping (Albertson's, Trader Joe's, CVS, etc.) and the freeway. The furnishings are really modern and comfortable, with a large TV in the living room and all bedrooms. I would have preferred a King bed in the master, but we made it work. The home was clean, and there were plenty of bath towels. The appliances are new and excellent quality. I hosted a family dinner one night. There were 9 of us all together, and we had room for all. I did have to get creative serving dinner, as there are no serving platters or bowls. Parking was available, but not close to the unit. Be prepared to walk a bit unless you get lucky with a close spot. I would definitely stay here again!
This place was such an amazing place to stay while on vacation. Within 15 minutes of both the strip and Fremont Street. Thoroughly enjoyed the homestyle feeling while on vacation. Highly recommend LV Rentals, they did an amazing job and look forward to staying with them again in the future.
A charming & very well equipped home in a great location. Suitable for four adults in three comfy bedrooms with two large bathrooms. Felt like a home everyday of our stay. l would stay there again. I've already recommended it to my friends!
|Rental Period
|Minimum Price
|Additional Nights
|Weekend Night
|Event Price
My Standard Rate
3 night minimum stay
for 3 nights
|Damage Protection Fee
|Cleaning Fee


text:
Fel-Pro 194-292 Chevy Head Gaske INLNE 194-230-250-292
Steel Core Laminate Head Gaskets, 4.175" Bore, .041" Compressed Thickness, GM Inline 6-Cylinder
Limited Supply: only 1 remaining
Availability: Same Day Shipping!
Shipping: Ships from Michigan.
Was: $61.10
Sale: $47.87
Save: 22%
Save: $13.23
Fel-Pro 194-292 Chevy Head Gaske INLNE 194-230-250-292 1025 Steel Core Laminate Head Gaskets
4.175" Bore .041" Compressed Thickness GM Inline 6-Cylinder Pit Stop USA offers Fel-Pro 194-292 Chevy Head Gaske INLNE 194-230-250-292 1025 at low everyday prices. Be sure to check out all of our products from Fel-Pro Performance Gaskets including Fel-Pro Performance Gaskets Cylinder Head Gaskets - Chevy Inline 6.
- 84113010255
- Fel-Pro Performance Gaskets
- Available
- Same Day Shipping!
- New
Shipping Information
- Item Requires Shipping
- 0.5 lbs.
- W7.2500" x H1.0000" x L31.7500"
- Ships from Michigan.
Customer Reviews
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text:
An Amazon.com employee was injured when he leaped off a building at the company's Seattle headquarters in what police said was a suicide attempt.
A married teaching assistant carried out six sex acts on a 15-year-old pupil during a night flight home from a school trip.
A married businessman killed his burlesque-dancer lover after learning she was planning to fleece him of his fortune, a court has heard.
An eighth person has died following a freak thunderstorm that hit Victoria in November.
Philippine President Rodrigo Duterte hit out at what he called "bulls**t" Western threats to seek his indictment by the International Criminal Court.
Tens of thousands of Cubans have gathered in Havana to pay their last respects to the late Fidel Castro.
Eleven people have been injured in a car and knife attack at Ohio State University's Columbus campus.
Green Party's Jill Stein requests a recount in Pennsylvania as Donald Trump's inner circle slams such "nonsense".
Germany's highest court has rejected an appeal filed by a man who was sentenced to four years in prison for his role in the murder of 300 000 people.
A US company has come up with DNA technology which could detect slavery in supply chains in the cotton industry. (VIDEO)
Cubans will gather on Havana's Revolution Square for two days to commemorate revolutionary leader Fidel Castro.
A Muslim woman made the semi-finals of the Miss Minnesota USA pageant while wearing a hijab.
A tiny baby pygmy marmoset monkey stolen from a wildlife park in Sydney has been recovered, Australian police say.


text: Following on form the OV10 thread in Aviation, I mused at the thought of the MoD doing the procurement for the Death Star. I decided that it would end up being called 'The Slightly Unwell Asteroid'. In a similar vein, what if the MoD did the procurement for Vaders Empire? What would Darth Vader actually end up with?


text:
Students who live in this rental at 1670 Market Street attend the following Unified School District (Unified School District) public schools:
|School Level
|Primary School
|Chinese Immersion School at Deavila
|Primary School
|Chinese Education Center Elementary School
|Primary School
|Fairmount Elementary School
3 More Primary Schools
|Middle School
|James Lick Middle School
|High School
|S.F. International High School
|High School
|Academy of Arts and Sciences
|High School
|Thurgood Marshall High School
11 More High Schools
|Mixed School
|Rooftop Alternative School (K8) (5-8 Mayeda Campus)
|Mixed School
|Claire Lilienthal Alternative School (K-2 Madison Campus)
|Mixed School
|Buena Vista/Horace Mann
9 More Mixed Schools
|Area Type
|This listing is priced
|Zip Code
|2% less than
|94102 Condos for Rent
|22% less than
|Van Ness - Civic Center Condos for Rent
|15% less than
|San Francisco Condos for Rent
|15% less than
|San Francisco Condos for Rent
|15% more than
|CA Condos for Rent
|Primary School
|27% less than
|Chinese Immersion School at Deavila Condos for Rent
|Middle School
|32% less than
|James Lick Middle School Condos for Rent
|High School
|27% less than
|S.F. International High School Condos for Rent
|Mixed School
|27% less than
|Rooftop Alternative School (K8) (5-8 Mayeda Campus) Condos for Rent
|Unified School District
|27% less than
|San Francisco Unified School District Condos for Rent


text:
Summary(Masterpieces of World Literature, Critical Edition)
Blood Wedding bestowed fame and fortune overnight on its author. In 1928, García Lorca read a newspaper account of a wedding that ended in tragic circumstances near Almería in southern Spain. He clipped the article, reread it five years later, and in a week finished his play, which became a hit in Madrid, Barcelona, and Buenos Aires. In Blood Wedding, García Lorca forcefully presents the theme of his three tragedies: Love that is unfulfilled because of the need to preserve honor and appearances results in death. A good-natured, hardworking young man contracts matrimony with a woman. The bridegroom is the only surviving member of a family that has been involved in a feud with the Felixes, and his mother is still overcome with a mixture of rage and fear that her only surviving son will meet the same fate. In rural Spain, where there were no secrets, it was known that the bride had been seeing someone else before the engagement. She is still madly in love with Leonardo (of the Felix family), who is married and the father of a boy. While the wedding celebration continues with singing and dancing, Leonardo rides away with the new bride. He is pursued by the groom, and the two men kill each other, thus causing the mother's forebodings to come true.
This simple plot summary does little to account for the sharp visual and verbal impact of the drama. García Lorca assigned a different color to each one of the scenes and characters. The groom's house has yellow walls, a pink cross accents the bride's dwelling, and the scene of the wedding has shades of whites, grays, and cool blues. Flowers are assigned to each character: carnations to the groom, a crown of orange blossoms to the bride. Folk lullabies are used for their musical effect and to advance the plot, and folk dances enliven the foreground of the wedding, while in the...
(The entire section is 761 words.)
Summary(Critical Survey of Literature for Students)
The Bridegroom's Mother is unhappy when she learns that her son wishes to be married to the woman he desires. In spite of her sorrow at losing him, she commands him to go buy fine presents for the Bride. The Bridegroom's Mother is also unhappy because the Bridegroom is her only surviving child. Her husband and her older son were killed many years before in fights with members of the Felíx family. Since then, the Bridegroom's Mother lived in fear that the only surviving man in her family, the Bridegroom, might also fall a victim to someone's knife or gun. She tells her son that she wishes he was born a girl, to sit in the house and knit instead of going out among men.
After the Bridegroom leaves the house to go buy gifts for the Bride, gifts to be presented when the parents meet, a neighbor stops to see his Mother. The neighbor tells the Bridegroom's Mother that there is bad blood in the Bride's veins, inherited from her mother. She also says that Leonardo, a member of the hated Felíx family and a cousin of the Bride, wooed the Bride unsuccessfully before his own marriage three years earlier. The Bridegroom's Mother grows uneasy at the news, but she determines to carry through her part in the marriage customs because her son is in love and because the Bride's Father owns rich vineyards comparable to those of her own family.
Meanwhile word of the proposed marriage reaches Leonardo, who still is in love with the Bride. In fact, he rides many miles to her house to see her whenever he has the chance. For some time both Leonardo's Wife and her mother realized that something was wrong. Leonardo is curt and sharp with his Wife for no reason at all, and he fails to take much notice of their child.
The next day the Bride's servant prepares her to meet with her father, the Bridegroom, and the Bridegroom's Mother in order to make plans for the wedding. The servant accuses the Bride of permitting Leonardo to visit late at night. The Bride, without denying the fact, merely indicates that she is not very happy at the prospect of marrying the Bridegroom.
After the arrival of the Bridegroom and his Mother, it is decided to have the wedding take place on the following Thursday, the Bride's twenty-second birthday. The Bride says that she will welcome the chance to shut out the world from her life and devote herself to the Bridegroom. A short time after the Bridegroom and his Mother depart, Leonardo's horse is heard neighing beneath the Bride's window.
The day of the wedding arrives, and early in the morning the servant begins to prepare the Bride for the ceremony. The Bride is not happy. When the servant begins to...
(The entire section is 1097 words.)


text:
2141 E Thistle Lane UNIT none,Auburn AL, 36832
Sold Price
3 beds , 2 Full baths
2,064 sq. ft
Status: Sold
Berkshire Hathaway HomeServices Preferred Real Estate
(334) 826-1010
Last Updated: 2/21/2017 5:38 PM
Description for 2141 E Thistle Lane UNIT none Auburn, AL 36832
Lovely Home situated on 1.84 acres in the City Limits of Auburn .....Very well maintained....numerous updates....lots of room....large bedrooms....updated bathrooms.......Basement would be great for playroom, media and office....Carpet in Bedrooms
Listing Information
Electric Water Heater
School Information
Room Information
Breakfast Area
Washer/Dryer Connection
Interior Features
Dishwasher, Disposal, Washer
Ceramic Tile, Vinyl, Carpet, Wood
Heat Pump
Heat Pump
Finished, Heated
Walls - Panelling
Exterior / Lot Features
No Pool
No Fence
Financial Considerations
Inside AccessTM
Current market conditions for 2141 E Thistle Lane UNIT none
Active Market Trends
Source:MLS DATA
Median list price$320,000
Days on market145
Properties for sale291
Sold Market Trends
Source:MLS DATA
Median sold price$230,500
Sales to list price9,789.0%
Properties sold218
Median sq ft price$114
Arrows indicate 90 day trend
Listing Price History for 2141 E Thistle Lane UNIT none
Auburn Real Estate
Auburn is located in Alabama. Auburn, Alabama 36832 has a population of 53,817.
The median household income in Auburn, Alabama 36832 is $37,590. The median household income for the surrounding county is $43,189 compared to the national median of $53,046. The median age of people living in Auburn 36832 is 23.1 years.
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(334) 826-1010
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text:
Is St. Michael The Archangel Catholic Church incorrect or missing key business information? Update your Hotfrog listing today.
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text:
It's the 1st of December and Milly Arnold is facing a miserable Noel. Newly single, the last thing she wants to do is join her settled sister and her young family for the first of their traditions: the writing of the Christmas lists. However Milly's nephews have other ideas and insist she write a list of her own (with illustrations to help Father Christmas decipher her terrible handwriting). Milly duly makes a request for a bunch of roses, some chocolate and a new pair of shoes. She doesn't expect to get any of it. However, that very day, a box of chocolates appears....
"Great little Christmas story."
There's only one thing worse than being jilted on the day of your wedding: being ditched a week before you have to be your friend's chief bridesmaid. If you are the jilted bride, at least you don't have to go through with the ceremony. As the recently dumped bridesmaid, you don't have the luxury of locking yourself in your bedroom and sobbing your heart out. Lizzie Jordan is waiting for her great break and in the meantime is happily settled with her boyfriend, Richard - until she hears those fateful words, "We have to talk".
Have you ever had your heart broken? How did you get over it? Did you delete his number and start again? Are you now friends with your ex? Perhaps you're godmother to his children? In which case, you're a weirdo and this book is not for you. But if you reacted with denial, begging or a spot of casual witchcraft, then you've come to the right place.


text:
ROSE - Rural Opportunities for Social Empowerment
About us
Rural Opportunities for Social Empowerment (ROSE) is a community organization promoting women's welfare through sustainable socio-economic opportunities. Together with its mother organization (non-profit Blossom) and women's empowerment program Poomalai, ROSE focuses on social entrepreneurial openings for rural and urban women. It builds off community micro-credit institutions and Self Help Groups to provide entrepreneurial services and women's economic capacity.
Contact information
Email: Log in to view email addresses.
Phone: 91 9750956860
Fax: 91 04562 269238
77,Sekkilar Street, Virudhunagar, Virudhunagar, State of Tamil Nādu, 626 001, India


text:
we are not our failures.
~la dispute.
Member since Aug 2011
just some random chill music.
100 4 20 tracks
a bunch of random songs i threw together that i can listen to on my phone
1,300 85 18 tracks
songs for and about that youthful journey to wherever your destination may be
for my obsession with California.
609 62 32 tracks
a playlist created out of my unhealthy desire to move to the glorious golden state-- songs written fr...
1,235 87 25 tracks
30+ calming and beautiful tracks including Coldplay, Bob Dylan, and Bright Eyes


text:
White House Run
By: Thomson Reuters
Welcome to the walkthrough for the iOS game White House Run, if you are looking for ways to make it to the next level, you've come to the right place!
The walkthrough contains the solution and will guide you through each of the levels with tips, tricks and cheats, to enjoy White House Run on your iPhone, iPad and Android devices.
Here you will find guides, answers, tutorials, helpful tips, as well as a discussion section for each level. It will help you if you're stuck!
We provide a community for gamers to discuss, learn and share anything about White House Run. Anyone can join in, share their experience and tips with others. If you have any questions, request or need extra help; feel free to ask by making a comment.
White House Run game app is developed by Thomson Reuters and available on iTunes and Google Play. For more details on the app, check out our review.
- App name: White House Run
- Seller: Thomson Reuters
- Category: Trivia
- Updated: Feb 22, 2016
White House Run iOS/Android Gameplay
Check out these tutorial for all levels of White House Run. We'll continue to update this list with the latest answers as new levels are released.
White House Run Answers
- Basic Game Guide Part One - dunkn dash game trailer
Hit like if this helped you!
Until the Walkthrough is completed, feel free to ask for help in the comments section.
Looking for a trick all your friends? Check out the White House Run cheats list to find collectibles guides, easter eggs and secrets.


text:
I will exorcise spirits, break curses, remove spells in the name of Jesus(42) 1 Orders in Queue
About This Gig
The Kingdom of Heaven is Here
Spirits only respond to one thing, the authority of God himself, the Lord Jesus Christ.
We have 16 years of experience casting out demons, we use no nonsense methods, without mumbo jumbo.
All we have to do is ask the Lord to deal with it, the trick is to know what to ask for! Our team includes followers of Jesus operating in all the spiritual gifts, including the biblical gift of discernment of spirits, and prophecy.
Tell us how you feel spirits are affecting you and we will pray for you.
(All proceeds put to charitable use, to help victims of extreme abuse)
Frequently Asked Questions
What is the skype "premium" appointment option?We provide one on one prayer ministry appointments via skype, on Thursday afternoon at or after 4pm GMT. You may have 4 appointments. If there is a queue you will have priority.
What is the "Standard" skype waiting room option?We provide one on one prayer ministry appointments, on Thursdays after 4pm, the prayer minister will help everyone but you will have to wait your turn. You may have 2 sessions.
How long will it take to answer my fiverr email?The "prayer activist" team meets regularly on a weekly or fortnightly basis, we aim to consider your requests, to pray about them and to reply as soon as we are able. This means that a response is unlikely to be immediate since the complete process of consultation does take some time.


text:
Watch Nosgoth Warband Pack trailer
An eternal war between human and vampires takes place over centuries, is known as Nosgoth war, in which both races face each other for survival. Nosgoth is an online multiplayer game that puts us in a war between two sides with their own skills: On the one hand we have the human faction, focusing on ranged combat, with weapons capable to crush vampires and including are pistols, rifles, bows and crossbows. And on the other hand we have the powerful vampires, specializing in melee combat, with awesome and unique abilities. This Warband package includes: instant access to game Nosgoth, 10000 Runestones (worth $ 50) for use in the ingame store, 2 invitations to friends, 2 silver chests and 2 gold chests, the chance to reserve the name of your clan, 25% shared boost to your team and a Warband Badge wich you can show during battles.
Access now and get 5 tickets as reward to join to weekly giveaways. Easy and for free!
Don't opt to win a game and Comment without register


text:
I will design an awesome Vintage or Retro LOGO in 12 hours(1)
About This Gig
Frequently Asked Questions
Differences between packagesThe design process is the same for all packages because it is the foundation of a good and timeless logo. It is the complexity of the project that will vary from package to package. Also, the files that you receive will be a key factor when you decide to buy a package from us.
Do you have a refund policy?Yes, we have a refund policy in the following situation: if you don't like the first concept without asking for any additional new designs, we can proceed with a mutual cancellation and you will be refunded. Else, if you request changes to the concept, we can't offer a refund.
Do I have full legal rights for the logo design?In order to have full copyrights (legal) over the design, please make sure you have ordered the Source Files. Otherwise, you will not be the sole owner of the logo, and we, Hivetex Design Studio will have the permission to resell the graphic symbol in the future.
What do I get for unlimited revisions?Unlimited revisions means you will get unlimited changes to the original concept (color changes, shape changes, font changes), not unlimited new logos. So you will get unlimited revisions to a concept, not unlimited new concepts.
How long does it take to design a new logo?We do normally take 24-48 hours to deliver the concepts. If you need 24 hours delivery time, then you need to order $10 extra for Extras fast service. Just checkout our All 3 packages.
What is Souce / Vector file and How does it works?Source files means completely editable file. It will come as .Ai or .EPS formats. its very high resolution file and with full editable layers. for access this, you must install Adobe Illustrator software in your PC/Mac.
Do you charge extra for Source / Vector file ?No. its completely free. However, if you need both vector files on same order , then we do charge extra. Because we do give 1 free vector file for 1 order.
Which designs includes in Social Brand pack and Stationary PackIn the Social brand pack. you will get cover page designs for Facebook, Google+, Twitter and YouTube. For Stationary, you will get Business card, letterhead and Envelope.


text:
Shocking Twist in Tashan - E - Ishq
The forthcoming chapter of Zee Tv's Tashan E Ishq will show that Yuvi is trying hard to sort out problem in Sarna family and winning Twinkle's friendship back.
Sarna family gets an offer of wedding where Yuvi manages to entertain the bridegroom family which makes Sarna family happy.
Yuvi dances with actress Aneri Vajani on the song Gall Gudia and not only this he brings entire Sarna family together on the dance stage.
Yuvi finds difficult to melt down Twinkle (Jasmin Bhasin)
Yuvi tries to prove Twinkle that he has changed but it is not quite easy for Yuvi to do it.
Sarna family starts to believe on Yuvi who is taking care of them after Kunj's death.
Keep Reading For More Updates Of Tashan – E – Ishq
Shocking Twist in Tashan - E - Ishq Reviewed by Telly Buzz on 08:32 Rating:


text:
I will get traffic for you from nigeria
About This Gig
i share blog and website posts online *mostly on facebook*. i have many facebook friends expecially from nigeria and on many facebook groups and pages that i share it to.
Order Details
massive traffic
i am in over 99 facebook groups with many facebook friends and pages. i will be sharing your blog.
- 500000 Visitors
- 5 Regions
Frequently Asked Questions
how many groups do you share it tomore than twenty


text:
Companies hoping to enter FTSE indices will have to float a greater proportion of their shares under a proposed tightening of the listing rules.
The FTSE Group launched a consultation yesterday for plans to raise the minimum proportion of shares that can be bought and sold, the so-called free float, for a company to list on Britain's leading indices.
Three FTSE 100 companies would be barred from inclusion in the index if the threshold were to be raised from 15 per cent to 25 per cent in line with the proposed changes.
The proposal is in response to criticism from


text:
Understanding the neurophysiology of fear extinction has important implications for the treatment of post-traumatic stress disorders. Here we report that fear conditioning resulted in an increase in AMPA/NMDA ratio as well as depression of paired-pulse facilitation (PPF) in neurons of the lateral nucleus of amygdala. These conditioning-induced changes in synaptic transmission were not affected by extinction training. D-cycloserine (DCS), a partial agonist at the glycine-binding site of the NMDA receptor, facilitated extinction and reversed the increase in AMPA/NMDA ratio without altering the depression of PPF when administered before extinction training. Extinction training, however, significantly increased the frequency and amplitude of miniature inhibitory post-synaptic currents and these effects were unaffected by the DCS treatment. Disruption of AMPA receptor endocytosis with a synthetic peptide containing a short C-terminal sequence of GluR2 (869YKEGYNVYG877, GluR23Y) specifically blocked DCS-induced reversal of AMPA/NMDA ratio and the facilitation of extinction. These results suggest that extinction training mainly increases inhibitory transmission leaving conditioning-induced excitatory association unaltered. DCS does not affect inhibitory transmission but reverses the conditioning-induced post-synaptic memory trace when administered before extinction training.
In the lateral amygdala (LA) and basolateral amygdala (BLA), excitatory pyramidal neurons use glutamate as a neurotransmitter (McDonald & Augustine, 1993; Sah et al.2003). During fear conditioning, LA neurons receive conditioned stimuli (CS) from the auditory thalamus and cortex and aversive unconditioned stimuli (US) from the thalamus. Associative activation of CS and US pathways which accompany behavioural learned fear results in long-term potentiation (LTP) of synaptic transmission from auditory thalamus and cortex to LA (McKernan & Shinnick-Gallagher, 1997; Rogan et al.1997), occluded LTP-induced presynaptic enhancement (Tsvetkov et al.2002) and increased synaptic GluR1 subunit of AMPA receptors (AMPARs) (Rumpel et al.2005; Yeh et al.2006). Interference with synaptic incorporation of GluR1 receptors in LA impairs LTP and fear memory formation (Rumpel et al.2005; Sigurdsson et al.2007).
Extinction is a behavioural paradigm in which the conditioned response gradually disappears if animals receive the cue only without pairing with a shock (Myers & Davis, 2002). The majority of patients with post-traumatic stress disorders (PTSD) exhibit long-lasting re-experience of traumatic events and, it is thought that dysfunction of fear extinction plays an important role in the development of clinical symptoms of PTSD. Recently, it has been shown that a partial agonist at the glycine-binding site of the NMDA receptor (NMDAR) D-cycloserine (DCS) facilitated extinction of conditioned fear and cocaine-induced place preference in rats (Botreau et al.2006; Ledgerwood et al.2003, 2004; Walker et al.2002) and enhanced exposure therapy of acrophobia (Davis et al.2006; Ressler et al.2004), social anxiety (Guastella et al.2008) and obsessive–compulsive disorders (Kushner et al.2007) in clinical trials.
Currently, there are two hypotheses concerning the mechanism of how DCS facilitates extinction. First, activation of a glycine site by DCS enhanced the function of NMDARs. Therefore, the facilitation of extinction by DCS could result from its enhancement of NMDA responses and subsequently the consolidation of extinction memory. Alternatively, DCS could facilitate extinction by unlearning because conditioning-induced increase in GluR1 was found to be reversed when DCS was infused into the amygdala before extinction training (Mao et al.2006). Consistent with the latter hypothesis, rats failed to show reinstatement when DCS was given immediately after extinction training (Ledgerwood et al.2004). However, failure to display reinstatement after DCS treatment does not necessarily mean the effect results from unlearning. It could be due to enhanced inhibitory conditioning of the context during extinction training. Further experiments by Woods & Bouton (2006) revealed that DCS did not eliminate renewal of conditioned fear suggesting that the original association was not impaired. Thus, it remains to be determined whether DCS facilitates extinction by erasing memory trace and/or by enhancing inhibitory learning. Despite these hypotheses, the effect of DCS on extinction has not been assessed electrophysiologically before. In the present study, we aim to unravel the effects of DCS on the excitatory and inhibitory synaptic transmission in the LA neurons and explore their relationship with the extinction of conditioned fear.
All procedures were approved by the Institutional Animal Care and Use Committee of the College of Medicine, National Cheng-Kung University, Taiwan. Animals were housed in cages of four rats per cage in a temperature (24°C)-controlled animal colony, pelleted rat chow and water were available ad libitum. The rats were maintained on a 12-h light–dark cycle (lights on 07:00 hours). All behavioural procedures took place during the light cycle.
Male Sprague–Dawley rats (175–200 g), were anaesthetized with sodium pentobarbital (50 mg/kg i.p.), and mounted on a stereotaxic apparatus and a cannula of 22-gauge stainless-steel tubing was implanted into the LA or BLA [anteroposterior (AP), −2.8 mm; mediolateral (ML), ±4.5 mm; dorsoventral (DV), −7.0 mm]. A 28-gauge dummy cannula was inserted into each cannula to prevent clogging. A TAT-conjugated peptide (GluR23Y, YKEGYNVYG) designed to impair AMPAR endocytosis was dissolved in 0.9% NaCl and infused into the LA or BLA (15 pmol/side) bilaterally 30 min before extinction training. The control peptide had the sequence AKEGANVAG (GluR23A). The dose was chosen with regard to Brebner et al. (2005).
Behavioural apparatus and procedures
Expt 1: fear conditioning
Rats were trained and tested in a stabilimeter device. Behavioural experiments of fear conditioning were performed in standard operant chamber (San Diego Instruments, USA). The acoustic startle stimulus was a 50 ms white-noise at 95 dB intensity. The visual CS was a 3.7 s light produced by an 8 W fluorescent bulb attached to the back of stabilimeter. The US was a 0.6 mA footshock of 0.5 s duration. Rats were placed in the startle test boxes for 10 min and returned to their home cages on three consecutive days to habituate them to the test chamber and to minimize the effect of contextual conditioning. Rats were placed in the startle boxes and received 10 light-footshock pairings with an inter-trial interval (ITI) of 2 min. Unpaired rats received the same number of CS and US presentations, but in an unpaired pseudo-random fashion. The rats were tested for fear-potentiated startle 24 h after conditioning. The test involved 30 startle-eliciting noise bursts presented alone (noise-alone trial) and 30 noise bursts presented 3.2 s after onset of the 3.7 s light (light-noise trials). The two trial types were presented in a balanced mixed order (ITI, 30 s). One hour after the test, the rats were sacrificed and amygdala slices were prepared for electrophysiological recordings and biochemical measurements.
Expt 2: fear extinction
Rats were trained with 10 light-shock pairings and retention of memory was tested 24 h later (test 1). On day 3, the rats received three sessions of 10 light-alone presentations (extinction training) and retention of memory was tested 24 h after extinction training (test 2). Context exposure control rats were exposed to the startle chamber for the same amount of time without receiving light-alone trials. DCS (20 mg/kg) or vehicle was injected intraperitoneally (i.p.) into the rats 30 min before extinction training. One hour after the test 2, the rats were sacrificed and amygdala slices were prepared for electrophysiological recordings and biochemical measurements.
Slice preparation
Male Sprague–Dawley rats aged 5–8 wk were decapitated and their brains rapidly removed and placed in cold oxygenated, artificial cerebrospinal fluid (aCSF) solution. Subsequently, the brain was hemisected and cut transversely posterior to the first branch and anteriorly to the last branch of the superior cerebral vein. The resulting section was glued to the chuck of a Vibroslice tissue slicer. Transverse slices of 500 µm thickness were cut and the appropriate slices placed in a beaker of oxygenated aCSF at room temperature for at least 1 h before recording. The aCSF solution had the following composition (in mm): NaCl 117, KCl 4.7, CaCl2 2.5, MgCl2 1.2, NaHCO3 25, NaH2PO4 1.2 and glucose 11. The aCSF was bubbled continuously with 95%O2/5%CO2 (pH 7.4).
Whole-cell patch-clamping recordings
Whole-cell patch-clamp recordings were made from the LA neurons. Excitatory post-synaptic currents (EPSCs) were evoked at 0.03 Hz by extracellular stimulation of fibres emerging from the internal capsule which originate in the medial geniculate nucleus of the thalamus and project monosynaptically to the LA using a bipolar electrode. Patch electrodes were pulled from thick-wall glass capillary (0.86 mm i.d., 1.5 mm o.d.) to a tip resistance of 3–5 MηΩ. The composition of the internal solution was (in mm): K-gluconate 140, KCl 10, EGTA 1, phosphocreatine 10, Mg-ATP 4, Na-GTP 0.3, Hepes 10 for recording neuronal properties and action potentials. The composition of the internal solution was (in mm): caesium methane-sulfonate 128, NaCl 20, EGTA 1, CaCl2 0.3, MgCl2 1, Na-ATP 3, Na-GTP 0.4, Hepes 10 for recording AMPA/NMDA ratio. The final pH of the internal solution was adjusted to 7.3 by adding 1 m KOH or CsOH; the final osmolarity was adjusted to 290–300 mOsm by adding sucrose. Records were low-pass-filtered at 2.5–20 kHz and digitized at 5–50 kHz. The signal was monitored and recorded with an Axopatch 200B amplifier. Online analysis and control of experimental acquisition was accomplished via a 586 (Intel)-based PC clone and a Digidata 1320 computer interface. AMPAR-mediated EPSC (AMPA EPSC) was evoked when the neurons were voltage-clamped at −70 mV, whereas NMDAR-mediated EPSC (NMDA EPSC) was determined as current amplitude at 50 ms after peak EPSC amplitude at a holding potential of +40 mV (Du et al.2008). AMPA/NMDA ratio was also measured by recording EPSCs at +40 mV before and after application of the NMDAR blocker D-APV (50 µm). NMDA EPSC was calculated by subtracting the response in the presence of D-APV from control response without adding D-APV. The peak of the AMPA EPSC was divided by the peak of the NMDA EPSC to yield an AMPA/NMDA ratio (Ungless et al.2001).
Data analysis
A single-factor ANOVA and post-hoc comparisons were used to analyse the differences in fear-potentiated startle and EPSCs among naive, unpaired and paired groups. Unpaired t test was used to analyse differences of AMPA/NMDA ratio and paired-pulse facilitation (PPF) between vehicle- and DCS-treated rats. All values are mean±s.e.m. p values <0.05 were considered to be statistically significant.
Alterations of excitatory transmission in the amygdala after fear conditioning
Rats were randomly assigned to naive, paired and unpaired groups. Rats in the paired group received 10 light-shock pairings and learned that light appearance predicted subsequent shock presentation. The unpaired group received 10 lights and 10 shocks in a pseudo-random manner and consequently did not learn light-shock association. Naive rats did not receive any training. As a result of fear learning, fear-potentiated startles were significantly elevated in the paired rats compared to those of unpaired and naive controls (Fig. 1a). An ANOVA comparing the difference between light-noise and noise-alone showed a significant effect of groups (F2,21=101.3, p<0.001). Newman–Keuls post-hoc analysis revealed that paired group was significantly different from both unpaired and naive groups (p<0.001) (Fig. 1a). Mean baseline startle was not different among groups (p>0.1).
Whole-cell recordings were made from the soma of visually identified pyramidal-like neurons located in the LA. Neurons were identified as projection neurons based on their morphology in caesium methane-sulfonate-containing electrodes. In potassium gluconate-containing electrodes, these neurons were also identified by their intrinsic electrophysiological properties, in particular spike frequency adaptation in response to depolarizing current injection. We determined whether the excitatory synaptic transmission in the LA neurons was altered after fear conditioning by measuring the relative contribution of AMPAR and NMDAR to the EPSCs which could minimize the effect of slice-to-slice variability and has been proven as a sensitive assay for detecting difference in the glutamatergic synaptic strength (Bellone & Luscher, 2006; Clem & Barth, 2006; Kourrich et al.2007; Ungless et al.2001). AMPA EPSC was evoked when the neurons were voltage-clamped at −70 mV whereas NMDA EPSC was determined as current amplitude at 50 ms after peak EPSC amplitude at a holding potential of +40 mV (Du et al.2008). In naive and unpaired rats, the AMPA/NMDA ratios were 1.43±0.11 (n=6) and 1.34±0.14 (n=6), respectively. The ratio was significantly higher in the paired rats (3.09±0.2, n=6, p<0.01) (Fig. 1b).
We also determined AMPA/NMDA ratio by measuring EPSCs at +40 mV before and after application of the NMDAR blocker D-APV (50 µm). NMDA EPSC was calculated by subtracting the response in the presence of D-APV from control response without adding D-APV. The peak of the AMPA EPSC was divided by the peak of the NMDA EPSC to yield the AMPA/NMDA ratio (Ungless et al.2001). Consistent with the above result, the paired rats exhibited a significantly higher AMPA/NMDA ratio (1.04±0.11, n=5) than the unpaired rats (0.55±0.08, n=5, p<0.01).
PPF is a measure of short-term plasticity widely used to probe for changes in presynaptic function because changes in PPF are inversely related to transmitter release. To determine whether the increased synaptic strength recorded 1 d after fear conditioning involved a presynaptic mechanism, we analysed PPF in slices from naive, unpaired and paired rats. Ratios of the amplitude of the second EPSC to the amplitude of the first EPSC were examined at different interpulse intervals. PPF at 30- and 60-ms intervals in the paired group was less significant (30 ms: 0.81±0.10; 60 ms: 0.96±0.10, p<0.05) than those of naive (30 ms: 1.44±0.16; 60 ms: 1.29±0.08) and unpaired (30 ms: 1.27±0.07; 60 ms: 1.33±0.10) rats (Fig. 1c). This result suggests that enhanced synaptic efficacy after fear conditioning is mediated at least in part by an increase in presynaptic release probability.
We next determined whether fear conditioning affected NMDA EPSC which was isolated in the presence of AMPA (CNQX 10 µm) and GABAA receptor (bicuculline 10 µm) antagonists. In agreement with a previous observation (Zinebi et al.2003), fear conditioning reduced the amplitude of NMDA EPSC (Fig. 2b) whereas the voltage-dependence of NMDA EPSC was not altered (Fig. 2a).
Alterations of excitatory transmission in the amygdala after fear extinction
We determined the changes of EPSC following the extinction of fear memory. Rats were trained with 10 light-shock pairings and retention of memory was tested 24 h later (test 1). On day 3, the rats received three sessions of 10 light-alone presentations (extinction training) and retention of memory was tested 24 h after extinction training (test 2). DCS (20 mg/kg) or vehicle was injected i.p. into the rats 30 min before extinction training. Figure 3a shows that vehicle- and DCS-treated rats exhibited significant reduction of fear-potentiated startle after extinction training. However, fear-potentiated startle in the rats treated with DCS in test 2 was less than that of the vehicle controls (p<0.05), confirming facilitation of fear extinction by DCS (Ledgerwood et al.2003; Mao et al.2006; Walker et al.2002).
Amygdala slices from vehicle- and DCS-treated extinction rats were prepared 1 h after test 2. A no-injection group which received conditioning followed by extinction training but without drug injection served as control. As illustrated in Fig. 3b, the AMPA/NMDA ratio in DCS-treated extinction rats (1.48±0.07, n=7) was significantly lower than that of vehicle-treated rats (3.04±0.26, n=7, p<0.01) and no-injection extinction rats (2.92±0.18, n=7, p<0.01). AMPA/NMDA ratios were not significantly different among paired (3.16±0.21, n=7), no-injection and vehicle-treated extinction rats (p>0.2), suggesting that extinction did not affect conditioning-induced increase in AMPA/NMDA ratio. In addition, there was no difference in AMPA/NMDA ratio among naive, unpaired and DCS-treated extinction rats (F2,12=0.16, p>0.5), indicating the reversal of conditioning-induced increase in the AMPA/NMDA ratio by the combination of DCS with extinction training.
Analysis of PPF at 30-, 60- and 150-ms intervals revealed no difference in slices from the paired, DCS- and vehicle-treated extinction rats (p>0.05) (Fig. 3c). The result suggests that neither extinction itself nor extinction in combination with DCS altered conditioning-induced depression of PPF.
In contrast to AMPA/NMDA ratio, conditioning-induced reduction in NMDA EPSC could be reversed after extinction training (Fig. 2b).
Effects of disruption of AMPAR endocytosis on extinction
A synthetic peptide containing a short C-terminal sequence of GluR2 (869YKEGYNVYG877, GluR23Y) has been shown to block LTD, depotentiation and AMPAR endocytosis in the hippocampus, nucleus accumbens and amygdala (Ahmadian et al.2004; Brebner et al.2005; Dalton et al.2008; Kim et al.2007). When GluR23Y was fused to the cell membrane transduction domain of the HIV-1 Tat protein (Tat-GluR23Y), it became membrane permeable and was able to impair extinction of fear memory. To determine whether GluR23Y actually has an effect on the LA neurons, we tested its effect on NMDA-induced internalization of GluR2. Amygdala slices were prepared and incubated with aCSF, GluR23Y (10 µm) or GluR23A (869AKEGANVAG877) (10 µm), a control peptide which lacks an effect on AMPAR endocytosis for 60 min. Figure 4 shows that the levels of internalized GluR2 were not significantly different among these three groups. NMDA application (20 µm, 3 min) caused an increase in internalized GluR2 (164.5±11.9% of aCSF control, n=4). Co-administration of NMDA with GluR23Y blocked the effect of NMDA (107.5±11.2%, n=4, p<0.01). In contrast, GluR23A (10 µm) had no effect on NMDA-induced GluR2 internalization (101.0±6.6% of aCSF control, n=4). This result demonstrates that GluR23Y is able to block NMDA-induced GluR2 internalization in the in-vitro amygdala slices.
We performed behavioural assessment to determine whether Tat-GluR23Y influenced extinction. Rats were divided into four groups: rats that received Tat-GluR23Y (15 pmol in 0.8 µl saline per side) or Tat-GluR23A, followed by DCS (20 mg/kg i.p.) or vehicle 30 min before extinction training. Memory retention was assessed 24 h after extinction training. A two-way ANOVA revealed no effect of group (Tat-GluR23Yvs. Tat-GluR23A, p=0.17) and drug treatment (DCS vs. vehicle, p=0.24) but a significant effect of interaction (p<0.05). Fear-potentiated startle in the Tat-GluR23A-DCS rats was significantly less than that in the Tat-GluR23Y-DCS rats (p<0.05) (Fig. 5a). Furthermore, startle potentiation in the Tat-GluR23Y-vehicle rats was comparable with that of Tat-GluR23A-vehicle rats (p=0.47) indicating that Tat-GluR23Y was without effect in the absence of DCS. Interestingly, in the Tat-GluR23Y rats, startle potentiation in the DCS-treated rats was comparable with that of vehicle-treated rats (p=0.31) indicating that Tat-GluR23Y specifically blocked the effect of DCS on extinction.
We determined whether Tat-GluR23Y affected excitatory transmission by recording the AMPA/NMDA ratio. In order to keep the integrity of the slices, Tat-GluR23Y was administered i.p. Thus, rats received i.p. injection of saline or DCS (20 mg/kg) followed 30 min later by extinction training. Tat-GluR23Y (3 µmol/kg i.p.) or Tat-GluR23A (3 µmol/kg i.p.) was injected 60 min before the administration of DCS. Memory retention was assessed 24 h after extinction training. A two-way ANOVA revealed no effect of group (Tat-GluR23Yvs. Tat-GluR23A, p=0.09) and drug treatment (DCS vs. vehicle, p=0.14) but a significant effect of interaction (p<0.05). Startle potentiation in the Tat-GluR23A-DCS rats was significantly less than that in the Tat-GluR23Y-DCS rats (p<0.05) (Fig. 6a).
Amygdala slices from each group were prepared 24 h after extinction training. Figure 6b shows that the AMPA/NMDA ratio was significantly lower in DCS+Tat-GluR23A-treated rats compared to the DCS+Tat-GluR23Y groups (p<0.001). These results suggest that Tat-GluR23Y itself has no effect but specifically blocks the effect of DCS on the AMPA/NMDA ratio.
Effect of DCS on inhibitory transmission
Rats were trained as described above and received an i.p. injection of DCS (20 mg/kg) or vehicle 30 min before extinction training. We compared miniature inhibitory post-synaptic currents (mIPSCs) among slices from the DCS-, vehicle-treated extinction, and paired (no extinction training) rats. As shown in Fig. 7, both mIPSC frequency and amplitude were lower in slices from the paired animals than those from the DCS- and vehicle-treated extinction animals (p<0.001), suggesting that extinction training increased the inhibitory tone. It should be noted that both frequency and amplitude of the mIPSC were not different between DCS- and vehicle-treated extinction animals indicating that DCS was without effect on mIPSC.
In the present study, we showed that AMPA/NMDA ratio in the thalamo-LA synapses was elevated in the conditioned but not unpaired rats. In addition, PPF of EPSC was depressed in the conditioned rats indicating that synaptic transmission underwent LTP after fear conditioning and the processes involved both pre- and post-synaptic mechanisms. These results were consistent with previous reports that neuronal changes mediating association between the cue and the aversive stimuli occur in the amygdala (Humeau et al.2003; McKernan & Shinnick-Gallagher, 1997; Rogan et al.1997; Sigurdsson et al.2007).
Because of clinical importance as an animal model of exposure therapy for the treatment of anxiety disorders, over the past several years extinction mechanisms have received increasing attention. In theory, there are three major models concerning the mechanisms of extinction. Extinction could be due to (1) devaluation of the US representation (Rescorla, 1973); (2) unlearning or erasure of the CS-US association (Rescorla & Wagner, 1972), or (3) formation of a new inhibitory CS/no-US association (Bouton & King, 1983; Bouton & Peck, 1989). Although extinction training significantly reduced fear-potentiated startle, notably we showed here that it affected neither conditioning-induced increase in AMPA/NMDA ratio nor the depression of PPF. In addition, extinction training resulted in an increase in frequency and amplitude of IPSCs. These results favour the view that extinction is a result of increased inhibitory learning but not erasure of the original excitatory association.
It is known that NMDAR plays a critical role in the extinction of fear memory and in the acquisition of new fear memory in previously conditioned animals (Falls et al.1992; Lee & Kim, 1998). Consistent with a previous report (Zinebi et al.2003), we observed that fear conditioning reduced the amplitude of NMDA EPSC. Interestingly, conditioning-induced reduction in NMDA EPSC could be reversed after extinction training, a result in contrast to that of the AMPA/NMDA ratio. PPF, an index of presynaptic transmitter release, was unaltered after extinction training suggesting a post-synaptic change of NMDARs. It is speculated that during fear conditioning excitatory synaptic transmission increased concomitantly with a decrease in inhibitory transmission. Therefore, the reduction of NMDA EPSC could be a mechanism that protects against excitotoxicity (Zinebi et al.2003). After extinction training, excitatory synaptic transmission remained elevated which could be counteracted by the increase in inhibitory transmission leaving the compensatory alteration of NMDARs unnecessary.
By binding to the strychnine-insensitive glycine recognition site, DCS enhances NMDAR-mediated synaptic response in the LA. Therefore, the facilitation of fear extinction by DCS could, theoretically, be due to its enhancement of inhibitory learning. In the present study, we found that conditioning-induced increase in AMPA/NMDA ratio was reversed by DCS in combination with extinction training. Analysis of frequency and amplitude of mIPSC revealed that there was no difference between DCS- and vehicle-treated extinction rats indicating that DCS did not affect GABAergic inhibitory transmission. Furthermore, Tat-GluR23Y, a synthetic peptide which inhibits AMPAR endocytosis, blocked only the additional reduction caused by DCS treatment, rather than returning the fear-potentiated level to that of fear-conditioned animal without extinction. These results coupled with the observation that DCS promoted receptor internalization (Mao et al.2006) imply that DCS facilitates extinction by erasing AMPAR expression but not by enhancing the inhibitory extinction memory. These results are in agreement with the observation that animals exhibit less reinstating effect after been treated with DCS before extinction training (Ledgerwood et al.2004).
However, a recent report suggests a different view showing that the DCS-mediated extinguished response could be renewed when the rats were returned to the context where fear had been conditioned (Woods & Bouton, 2006). Somatosensory input is strong and it is likely that US-alone presentations cause robust depolarization to LA neurons and then to the BLA neurons. Projections from BLA to the mPFC synapse on both principal pyramidal neurons and parvalbumin-immunoreactive GABAergic interneurons (Gabbott et al.2006). The inhibitory interneurons innervate the soma and initial axonal segments of mPFC pyramidal cells leading to the inhibition of spontaneous firing which in turn disinhibits LA neurons and reinstates the fear response (Buffalari & Grace, 2007; Rosenkranz et al.2003). The reinstatement was attenuated by DCS because conditioning-induced increase in AMPA/NMDA ratio was eliminated. However, the increase in glutamate release, elicited by previous conditioning which was unaffected by DCS treatment, may drive downstream structures to exhibit fear response when the rats are returned to the context in which fear has been conditioned (renewal effect).
An interesting characteristic of DCS is that systemic administration of DCS facilitated extinction at the first time but not the second time (Langton & Richardson, 2008). One possible explanation for the failure of DCS to enhance re-extinction after re-training is that DCS pre-treatment induced the development of tolerance (Richardson et al.2004). Alternatively, neural circuitry associated with re-extinction is different from initial extinction. In view of the fact that during re-extinction mPFC lesions caused greater resistance to extinction compared to mPFC lesions during initial extinction, the mPFC may play a more prominent role in re-extinction than initial extinction whereas amygdala is not required for re-extinction (Morgan et al.2003). In this respect, our result showing that inhibition of AMPAR endocytosis in the amygdala specifically blocks DCS-mediated facilitation of extinction seems to be consistent with the latter explanation.
In summary, using electrophysiological and behavioural assessments, we tackled fundamental issues regarding the extinction of conditioned fear. First, we provided direct evidence that synaptic potentiation associated with fear conditioning was the result of a combination of pre- and post-synaptic modifications of excitatory and inhibitory transmission. Second, we found that conditioning-induced increase in AMPA/NMDA ratio and depression of PPF was unaffected by extinction training. Third, we found that DCS treatment plus extinction training reversed conditioning-induced increase in AMPA/NMDA ratio without affecting the depression of PPF. These results suggest that DCS does partially erase the original excitatory association. The context-dependent renewal of learned fear after DCS treatment could be explained by the inability of DCS to reverse conditioning-induced increase in presynaptic transmitter release.
This study was supported by grants N08I97N from the National Health Research Institute, NSC96-2628-B-006-005-MY3 from the National Science Council and Landmark Project (R026) of the National Cheng-Kung University of Taiwan. We thank Dr Min-Der Lai for critical comments on the manuscript.
Statement of Interest


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Sales Advisors - Automotive/Motor Trade
3 days left
- Recruiter
- Search International Ltd
- Location
- London
- Salary
- 24500.0000
- Posted
- 02 Feb 2017
- Closes
- 02 Mar 2017
- Sector
- Engineering
- Contract Type
- Permanent
- Hours
- Full Time
The Role
For our Sales Advisor / Executive position, our client is looking for a self-starter who is a highly motivated, flexible, personable, energetic and highly organised. The Sales Advisor will report to the Manager.
The successful candidate will be crucial in launching the newest model in their market. They will be responsible for introducing the product to buyers and influencers and quickly growing the sales footprint of the new model their market.
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- Contribute at Test Drive and Test Drive Events with reservation holders and potential customers
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- Bachelor's Degree or any other Higher Education preferred. In lieu of a degree, relevant experience within the Automotive, Luxury, Customer Service or Retail industry.
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- Sales experience and Customer-facing experience required.
- Excellent Communication skills, both oral and written. Fluency in English required
- Valid driving license (2 years minimum)
Our client is committed to a policy of equal employment opportunity.


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Working Together To Strengthen Agency Collaboration January 8, 2010 January 8, 2010 Offered Jointly By: Humboldt State University, Northern California Training Academy UC Davis Extension Funded by California Department of Social Services Weaving Good Relations
Stepping onto the Path * Understanding The Past * Valuing The Present * Creating The Vision
Connecting Where did we come from? Where did we come from? Where are we going? Where are we going? Where did we come from? Where are we going? Connecting
Connecting Where did we come from? Where did we come from? Where are we going? Where are we going? How do we increase our awareness and potential to increase outcomes? What are our challenges?
What are our values?
Traditional Indian Values vs. All American Values Mainstream Values Clan/communal emphasisIndividual Emphasis SharingWinning Present-time orientationFuture Orientation SpiritualisticMaterialistic Time non-awarenessTime awareness Harmony with natureConquest of nature PassiveAggressive Giving/spendingAcquiring/saving Appreciates/honors silenceAvoids silence Respect of other religionsConverting/proselytizing (Source: The Indian Child Welfare Act, Handbook by Rose-Margaret Orrantia; Cultural Awareness; the Indian Perspective, Marilyn Robinson).
Tribal Values? University Values? County Values? Community Values? Family Values?
In these systems, who is the expert?
What is our history? What Events Have Shaped Our Current Cross-Cultural Relations With Native Communities?
The Apology Remarks of Kevin Gover, Assistant Secretary- Indian Affairs Department of the Interior at the Ceremony Acknowledging the 175th Anniversary of the Establishment of the Bureau of Indian Affairs September 8, 2000
What are our contributions?
What are our successes?
Examples of collaborative successes: Riverside Court-County-Tribal Alliance Native American Justice Project Tribal STAR This Conference HSU American Indian Programs
Tribal STAR is a result of a partnership between the SDSU School of Social Work, Academy for Professional Excellence and: Southern Indian Health Council SD HHSA Indian Specialty Unit Indian Health Council Santa Ysabel Band of Digueno Indians SD HHSA Independent Living Unit YMCA YFS South Bay Community Services San Diego Youth & Community Services Intertribal Court of Southern California, Southern CA Tribal Chairmen's Association County of San Bernardino Childrens Services County of Orange SSA Soboba Band of Luiseno Indians Casey Family Programs
CollaborationCoordinationCooperation Commitment to common mission Understanding of compatible mission Individual interpretations of mission Mutual goals and plans Some planning and division of roles Informal structure Well-defined communication channels operating on many different levels Communication channels established Information shared as needed Resources contributed and pooled or jointly secured Resources available Limited or no resources (Jones, et al., 1999).
Tools to consider: Mediation as conscious process.
Position vs Interest Neutral Reframe
Tips for Following Protocol Disproportionality Program Logic Model
Timing is everything. Re-Center around the consumer, focus on an outcome.
What are our concerns? What are our strengths?
Cornerstones Have direction and keep moving. Respond to community needs. Elevate partnership strengths, successes, and leverage PR for sustainability. Hold the family together.
Know who you are Where you are from You don't know where you are going Until you know where you come from
Connecting Tribal STAR, SDSU School of Social Work Academy for Professional Excellence, Pacific Mountain Philanthropy firstname.lastname@example.com@aol.com760-774-4782 firstname.lastname@example.com
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Manchester United's players are sleeping in special oxygen pods in between training sessions in an effort to speed up recovery times and reduce the threat of recurrent injuries.
The move is one of the latest examples of Louis van Gaal's meticulous and obsessive overhaul of United's Carrington training base and the club's fitness, training and rehabilitation programmes.
With double training a regular feature since Van Gaal's arrival at Old Trafford in July, players can now sleep in oxygenated chambers at lunchtime after a morning session and before the afternoon run-out to help to enhance performance levels and improve their quality


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Canadian Stocks Sink to Two-Week Low as Banks, Energy Retreatby and
Royal Bank joins global selloff in lenders on credit concerns
Oil producers retreat as crude hovers around $30 a barrel
Financial and energy companies dragged Canadian stocks lower for a third day, as equities worldwide sank amid deepening concerns about the health of the global economy.
The Standard & Poor's/TSX Composite Index slumped 2 percent to 12,282.65 at 4 p.m. in Toronto, the lowest level since Jan. 15. The benchmark gauge has fallen 5.6 percent this year, making it the second-best performing developed market tracked by Bloomberg, after New Zealand.
While the U.S. market staged an afternoon rebound to finish Tuesday little changed, Canada's resource-rich benchmark equity gauge was held back by plummeting oil prices. As crude fell more than 4 percent to settle near $28 a barrel, energy companies retreated for a third straight day and all 55 stocks in the industry declined. Ensign Energy Services Inc. plunged 10 percent to its lowest level since 2000.
Banks contributed the most to losses, joining a selloff in global financial shares amid growing concerns that the rout in oil will weaken lenders' balance sheets. Lenders in the gauge declined 2.2 percent and only five stocks in the group advanced. Royal Bank of Canada, Toronto-Dominion Bank, and Bank of Montreal all fell more than 2.3 percent.
Utility stocks fell 4.8 percent, the biggest decline since 2001 and the most among the 10 sectors. Fortis Inc. tumbled 10 percent, the most ever, after agreeing to buy ITC Holdings Corp. for $6.9 billion in cash and stock, adding its high regulated returns in what will be the largest Canadian takeover of a U.S. utility.
Industrial companies rose 1.1 percent for the biggest gain in the S&P/TSX on Tuesday. Bombardier Inc. increased 2.6 percent, as the company entered partnership with Turkish manufacturer Bozankaya to produce high-speed trains.


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The Jordan Melo M12 debuted a few weeks ago and features specific technologies that feed off of Anthony's game. It features a lightweight, flexible upper that......
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Hon putting your babe in with you in bed isn't 'bad'. It's only an issue if you dont want to be doing it. there's absolutely nothing 'bad' about it.
Having said that, we went to sleep school
And it had been great for us.
Hon putting your babe in with you in bed isn't 'bad'. It's only an issue if you dont want to be doing it. there's absolutely nothing 'bad' about it.
Having said that, we went to sleep school
And it had been great for us.
Well I was totally disappointed. I only did half a day and they told me to go home as its a feeding issue aparently (DD also has low weight gains)
I asked for some other techniques I could try at home and was told no we just continue to try "pat & ssshhh" which my DD doesnt respond too. I was also told I was not to bounce her on my fitball (which is the only way I can currently get her to sleep) as it is like "shaking my baby"
So they took away my one thing that works, gave me nothing new to work with and told me to leave. So I came home feeling more helpless...
My DD does have other issues as I said and other people I know have said its great thats just my expereince.
Oh and there was NO CC they told me to pick her up straight away if she was upset.
I'm sorry they didn't help Jess, how disappointing How old is your little one? I'd be ignoring their advice to be honest... really it can't be that bad to bounce gently on a fit ball
I hope the experience is different for me. The only way I am getting through the days now is clinging to the hope that they help us. On lucky nights (maybe once or twice a week) I am getting about 4 hours sleep. Other than that I get maybe one, two hours then am up all night. Last night I got NO sleep. He woke up when I went to bed and fussed all night. He doesn't sleep during the day either Doesnt help I have a hopeless DF that does nothing but make everything harder. Feeling so tired and depressed.
Chloe a lot of other people have found them to be really good.
They were friendly and made sure you were comfortable with each step, pretty much I made the decisions and they helped with them.
Are you on their "on-call" list? if you get your mchn or gp to write a referral you should get in quicker too.
I went when DS2 was 13 months and I was 7 weeks pregnant.... I loved it. They were so supportive and we used a really gentle method with DS. It worked wonders. I didn't want to leave.
Did they offer you anything other than the pat&sshhh?
I still don't know what to do!
It worked so well for us and DS responded really well.
TBH I wouldn't go with a baby younger than 12 months....
How did you go at the sleep school we are thinking of going there for a 4 night stay
I have just returned home from a 4 day program at Tweddle. This is one of the threads I read prior to going in to get an idea of what was going to happen. Perhaps cry it out was used in the past or 'controlled crying' but that IS NOT what happens. This thread worried me and I wanted to write a post stating how wrong some of the info is! (I know it is 2yrs from when it started).
It has saved my LIFE and SANITY!
NOTE** if you want it to work for you, you need to have the following;
- positive attitude
- willingness to let others help
- desire to help your baby NOT just yourself
- ability to set aside your pride
the ladies are there to help if you dont listen (or go behind their back and do something they specifically said not to) any attempt to change anything will fail.
ok so:
my breastfed bub had never slept more then 3-4hours, noone could babysit and he refused solids. Oh and I breastfed him to sleep eek.
Using a soothing technique (patting and shushing) I was able to change his association of breast and sleep.. That in turn meant he didnt wake up expecting to be on the breast and could resettle himself. Needless to say he slept 10hours - the SECOND NIGHT!
NO cry it out or controlled crying - although on odd occassion if you have been trying to settle for a long period of time you step out of the room for 10-20sec. This says to bub that you are not going to play their game or give in.
WATCH for the tired signs.. By the time my bub rubbed his eyes it was over he was wrecked. By careful study I learnt that his early signs were needing to be on me, biting and staring.
DO NOT go by set times. Bub can be tired 10min before your set time and so by the time you try to settle they are over tired and not having a bar of it.
Breastfeed upon waking - never just before bed
If bub wakes up before an hour is up (during daytime) always try to resettle.
Always try to resettle at night before offering the breast.
I cannot tell you how much Tweddle helped and at no point was my bub distressed. I went there willing to accept help and lose a bit of sleep. My baby has changed so much in just 4 days. They help babies and toddlers and deal with a wide range of issues not just sleep.
Thankyou Tweddle!
Starfish30 (16-08-2013)
Ok parents so iam writting this review on the third and final night inside twiddle.so CONs first,1. yes walls are paper thin u can hear everyone and every flush.2. Change of shifts 4 of them.. means that no consistency.3. No "one policy" application. every person has their own ideas they wanna try (this can lead to wrong message to the baby). We almost told off one lady tonight.4. No followups after u leave.Ok now the PROs1. Almost all the staff is very friendly and helpfull2. No matter how bad a sleeper your child is ull find worse in here.. ull learn from their mistakes3. Different opinions mean new methods u can try with your little one see which works as we now know every child behaves differently to the same technique.4. You quickly realise your mistake/s at home.So to end this... Tweddle is no mirracle stick. Although most parents can see the difference straight away (unfortunately not us), it will take few consistant weeks afterwards.Hope this helps.
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Winnie Rider has the kind of fantasies all girls have, but most are never ready to admit. This hot blonde wants to be bound. She wants to have her big tits roughly handled. She wants her beautiful ass to be plundered. She wants to feel her tight pussy crammed full of cock. And if it all comes with a ball gag, some corporal punishment and miles of rope holding her firmly in place then it's even better as far as she is concerned.Format: mp4Duration: 52:47Video: 1280x720, AVC(H.264), 2681kbpsAudio: 109kbps
Winnie the Hun Part 3
Winnie the Hun 2


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First, he became known for his leather jacket and a black balaclava. Then he stunned the halfpipe world with unprecedented amplitude.
Joffrey Pollet-Villard, 22-year-old, broke the world record with a hit over 8 metres high. To him, it was more than a show. It's his way of living and protecting the spirit of freestyle skiing.
You got injured in New Zealand a year ago. What have you done since that time?
I took the time to relax and think about my way of skiing and about freestyle skiing in general. This unexpected break convinced me of what I wanted to do: go as high as possible, clean my tricks, bring new tricks to pipe skiing, have creative and original runs. I want to show that halfpipe can be fun, not only a rat race of rotations. Halfpipe riding is not only about competition.
You are one of the few skiers to say that.
Wingtai Barrymore is on the same wavelength. I'm looking for style and amplitude; I'm not working on the technical side, polishing my tricks on airbags. I want the spectators to think: This is beautiful!
Even if I know I can't get a medal like that. Maybe I'm stuck in the past, but I loved Simon Dumont throwing cork 900 on the first hit… he used to go so high, it was risky and beautiful.
Halfpipe is a dangerous and demanding discipline.
That's why I like it so much. Being high up there is scary… and landing on your feet is so satisfying. It takes a lot of work to understand how the curve works, how it can propel you into the air.
Since I was a kid my goal was to be at the X Games. I've done that, I finished 5th at Aspen in 2013, now I want to explore the other facets of freestyle skiing: urban jibbing, backcountry freestyle… We have such beautiful mountains in my hometown in La Clusaz.
Did you think of quitting halfpipe?
Yes. I don't want to ski like the others, but I want to share what I like, show the spirit of freestyle skiing… that has nothing to do with trampoline training. Halfpipe is not only about double rotations or even triple. This focus on technique will quickly limit our sport and turn into a new version of acro skiing, a discipline now dominated by gymnasts. I ski a lot, I don't train on trampoline or airbags, I spent time on the snow and that's how I can jump so high. We are skiers before being freestylers.
Can halfpipe evolve in its format?
Danny Davis' Peace Pipe or the B&E Inventational are a good example of what can be done towards more fun and playfulness, mixing pipe with modules or rails.
Why wear a black balaclava?
I like the idea of being hidden. I want to be judged only on my skiing.
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text: Seller: periodconcepts1 (2,458) 100%, Location: Salem, Oregon, Ships to: US, Item: 170916537190 Five New Fancy Brass Door Strike Plates - Antiqued Missing your door's strike plates? These new ones are cast from the original fancy Victorian plate and are a rather dark antiqued brass. They measures 7/8" wide and 3 3/8" tall. The openings are 1/2" by 3/4" (top) and 1/2" by 1 1/8" (bottom). Shipping is free! We believe in the wonderful artistry and fine quality hardware produced by 19th century craftsmen. Unless stated otherwise, every item on these pages was designed and made right here in our American past. And we'll guarantee that you'll be delighted with your purchases, which are always backed by our money-back return policy. See my other listings for more great items! We never charge a handling fee, and we'll get your item sent off within one business day! We accept Paypal. This will ship to your home in the USA for FREE. If not completely satisfied with your item, simply return it to us in the original packaging and we'll refund your purchase price. To view the showcase you need Flash 6 or higher. Click here to Install it now.--or-- View Other Listings for sarahandkeith txzj pxzj1101 txzj67 Material: Cast Brass, Style: Victorian, Original+or+Reproduction: Reproduction, Hardware+Type: Door Hardware


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In this webinar we'll provide an overview of the Nokia X platform and product offering. Then we'll dig into monetization opportunities offered by Nokia In-app Payment combined with Nokia's extensive operator billing network. We'll show you how you can benefit by bringing your apps to Nokia Store, take advantage of it's unique monetization capabilities and make your app stand out from the crowd.


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We don't Support the unethical trade practices adopted by DREAMZ INFRA INDIA PVT LTD.
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Apartments in Mumbai /
Mumbai /
Kalyan East /
Gopika Enclave
Gopika Enclave is located in Mumbai. This project provides all features that a contemporary property buyer would want to have. Gopika Enclave is a part of the suburban locality of Kalyan East. Kalyan East is connected to near by localities by wide roads. inhabitants of the locality have easy access to public facilities like schools, colleges, hospitals, recreational areas and parks. The facilities within the project are thoughtfully created.
Units and Interiors:
Readily available housing options here include apartments of various dimensions. The size of Property available in this project varies from 590 sq.ft. to 740 sq.ft. Gopika Enclave floor plan provides best utilization of the space. From elegant flooring to airy balconies, standard kitchen size and superior-quality fitments, every little detail here gives it an attractive appearance. The master plan of Gopika Enclave includes a large number of desirable amenities that collectively guarantee a serene lifestyle. Fenced play-area has been provided for kids. Complete power supply has been provided with complete security via high-quality wires and PVC conduits. Security is certainly one of the biggest concerns while buying an home. Thanks to its security features, Gopika Enclave is a great place for you and your family.
Rai Residency Pvt. Ltd is one of the most prominent and trusted names in the real estate industry of MMR and has developed over 1 million square feet of residential and commercial spaces in and around KDMC. The group has set for itself very ambitious growth plans for the coming years. As part of its CSR activities, the group plans to set up a Vrudha Ashram and a degree college in the near future.
Rai Residency Pvt Ltd. is an AVERAGE builder. It has 1 on-going projects with total inventory value of N/A
This project has minimal amenities and hence is recommended for location which has high accessibility and high lifestyle ratings.
This information is being processed. You can still view the details of the project in the meantime.
Rs.24.8 Lac - 26 Lac
@ Kalyan East
Reliable Homemakers
Rs.26 Lac - 58 Lac
@ Kalyan East
Rai Residency Pvt Ltd
Rs.25.3 Lac - 55 Lac
@ Kalyan East
Rai Residency Pvt Ltd
Rs. 58.32 Lac Onwards
Apartments @ Bommanahalli, Bangalore
1.95 Cr - 2.55 Cr
Villas @ Konanakunte, Bangalore
74.2 Lac - 1.75 Cr
Apartments @ Ulsoor, Bangalore
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Looking for Ipad app log events Freelancers or Jobs?
Need help with Ipad app log events? Hire a freelancer today! Do you specialise in Ipad app log events? Use your Ipad app log events skills and start making money online today! Freelancer is the largest marketplace for jobs in the world. There are currently 17,764 jobs waiting for you to start work on!View Top 6 Graphic Designers View Top 6 Web Developers
Ipad app log events Jobs:
|Price (USD)
|Price (USD)
|We need to build the backed interface for this calendar on http://126.96.36.199:9000 for users to add events to dates then export the code with the events added undestend all/
|We need to build the backed interface for this calendar on [url removed, login to view] for users to add events to dates then export the code with the events added undestend all/
|PHP, Java, Javascript
|Feb 25, 2017
|Today6d 20h
|Design a facebook cover & facebook events
|Design a facebook cover & facebook event. Must be correct size for facebook. We want our logo included. "One Entry Price" "All Rides & Entertainment FREE" "3rd 4th & 5th of June 2017" It can be an illustration or you can use photos. Or you can use both illustrations and photos. I have uploaded our logo. I have uploaded photos that you can ...
|Banner Design, Graphic Design, Logo Design, Website Design
|Feb 25, 2017
|Today2d 19h
|PHP Script to run Google Search and log results
|I want you to create a PHP script that will use CURL commands from PHP to generate a Google search and then record the results. Basically, I want to simulate user searches on Google. I'll run this with a CRON job on my server, and have it run a VERY FEW times/day (4-6) to simulate a search. The script will randomly choose from a set of predefined search words each time it is run, and will...
|Feb 24, 2017
|Today6d 14h
|Write a report/training log/ article about endurance training -- 2
|- Introduction/background and goal for the training period - Training plan for 6 weeks - Two different kinds of endurance training workouts - Theory: Heating, endurance, strength, flexibility exercise and diet. - Training Principles (show that you have used those in training planning). - Result of the 6 week training, use tables and statistics (make a fake, but realistic diary o...
|Report Writing, Articles
|Feb 23, 2017
|Feb 23, 20175d 13h
|Create a Video for CS Explosivo Events
|i would Like a short promotional video about my company [url removed, login to view] our info is in Greek i translate it in English from google translate if you want more info sent me a message The CS Explosivo is a group that operates in the field of leisure and entertainment. We have the ability and experience to help you organize unique events and mainly private parties which is our speci...
|Video Services
|Feb 23, 2017
|Feb 23, 20175d 3h
|Write a report/training log/ article about endurance training
|- Introduction/background and goal for the training period - Training plan for 6 weeks - Two different kinds of endurance training workouts - Theory: Heating, endurance, strength, flexibility exercise and diet. - Training Principles (show that you have used those in training planning). - Result of the 6 week training, use tables and statistics (make a fake, but realistic diary o...
|Report Writing, Articles
|Feb 23, 2017
|Feb 23, 20175d 1h
|Troubleshoot/Fix a Paypal Chained Payments Gateway to function with Events Manager Pro Bookings in Wordpress
|I had a freelancer create a Paypal chained gateway for the Events Manager Pro Plugin so that I can have users book and pay for tickets for events on my site. The plugin seems to work great except that emails are not going to registrants after they register/book the events and their transaction information is not appearing in our bookings tables although the record does show as a Booking.. This is...
|PHP, WordPress, Troubleshooting, Software Architecture, PayPal API
|Feb 21, 2017
|Feb 21, 20173d 12h
|Build a Website to show events on a map
|It's a page that shows event on a map, each Event has: - Title (text) - Date (DD/MM/YYYY) - Location (on the Map "Geo coordinates") - Type (nested) - Hyperlink (clickable link to a webpage) The main page has: - Map control: (in satalite view, it has the normal control the map) and I can add pins on it (as Events). - Search field: A text field that has autocomplete and sh...
|PHP, Website Design, Graphic Design, MySQL, HTML
|Feb 20, 2017
|Feb 20, 20172d 6h
|Soap4Sale log
|I need a logo for our website and order forms: We sale laundry soap, dish soap, body wash, fabric softener and other stuff in 5 gallon buckets
|Graphic Design, Logo Design
|Feb 19, 2017
|Feb 19, 2017Ended
|Eventboard - Events Made Simple
|EventBoard is a generic platform to make communication between event holders and attendees simpler, more straightforward, and more interactive. In most cases, events holders have their own websites to attract and signup members, but when it comes to the actual gathering, more often than not they have a hard time communicating with everyone. Furthermore, it takes an abundant amount of time and mone...
|PHP, Website Design, Graphic Design, HTML
|Feb 19, 2017
|Feb 19, 20171d 11h
|Create Recurring Events in Website
|Store 1 line in the event_time table with the from/to date range & the correct day of week. That will resolve correctly
|PHP, Javascript, Codeigniter, MySQL, HTML
|Feb 19, 2017
|Feb 19, 20171d 6h
|Global Events
|i have a profesional soundline company. I have audio systems and djays, photography and video. I go to events (birthdays, weddings, proms etc) and i need a logo for this company. Name of the company is Global Events
|Graphic Design, Logo Design
|Feb 19, 2017
|Feb 19, 2017Ended
|mobile cross platform app - events - 17/02/2017 14:01 EST
|I need a very basic app developed. It needs to do the following: - Allow users to login using facebook or register - Allow users who forgot password for such to be resolved via email - require users to complete personal details - input details - allow users to create events and geotag location where such will be - allow users to search events by area and certain criteria - allow users to r...
|Mobile Phone, iPhone, Android
|Feb 17, 2017
|Feb 17, 2017Ended
|Home Events Sharing - Develop Web APP, iOS APP and Android APP
|This is a CUSTOM project. Please read thoroughly, attention to detail is required. This will be an portal where users can POST & HOST / PURCHASE various events (home based parties) that they advertise. Develop Web APP, iOS APP and Android APP Major deliverable's are: A. Applications as per requirements B. Code & Documentation C. Basic troubleshooting guide D. How To Use fea...
|Mobile Phone, iPhone, Android, Agile Development, App Developer
|Feb 15, 2017
|Feb 15, 2017Ended
|Developing or an apps for promoting marketing projects , start ups business with payment gateway and users can upload their project delete, edit , save , log in , register , using social media ,add project titles receive feed backs , answer questions , et
|I need an iPhone/iPad app. I would like it designed and built. The apps is like a marketing apps or advertising for promoting peoples projects globally but the other part of the project is to allow the user to invite contacts from other emails or social media like google + , Facebook , likeden, Facebook , twitter , yahoo etc , but also to alert user or remind them about any projects going on if th...
|Mobile Phone, iPhone, iPad, App Designer, App Developer
|Feb 15, 2017
|Feb 15, 2017Ended
|Design a log for "iDocus"
|we need to design a log for "iDocus"
|Graphic Design, Logo Design
|Feb 15, 2017
|Feb 15, 2017Ended
|Telephone Marketing Appointments for Timeshare Liquidation Events
|I need an experienced telemarketer to call through leads that I supply and book timeshare owners to come to a seminar in their area. We are a timeshare contract cancellation company with real proven results. Our services are 100% guaranteed and we haven't lost a case in the 6 years we've been in business. We have an A+ rating with the BBB and the only real testimonials in the busine...
|Mobile Phone, Telemarketing, Sales
|Feb 14, 2017
|Feb 14, 2017Ended
|Logo Design- Intrigue Events
|I need 2 versions of the same logo designed with different names. I have examples of what I looking for.
|Graphic Design, Logo Design
|Feb 13, 2017
|Feb 13, 2017Ended
|events registration / membership
|Tour registration Player Registration Coach registration Event Page: Every section needs a corresponding admin section The tour page is for companies that host tournaments to sign up with our services. Basic information for Tour Registration Tour name Contact First Name Contact Last Name Contact Email Contact phone This section will be added too at a later date like paym...
|PHP, Website Design, Graphic Design, HTML
|Feb 13, 2017
|Feb 13, 2017Ended
|OLPHCAB.org - can't log in to front end
|I have a site at [url removed, login to view], and while I can log into the /administrator end, I can't log into the front end. (500 error)
|Feb 13, 2017
|Feb 13, 2017Ended
Showing 1 to 20 of 6059 entries
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Decoding Darkness
The Search for the Genetic Causes of Alzheimer's Disease
By (author) Rudolph E. Tanzi Ann B. Parson
Format: Paperback
Normal Price: $30.45
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Plus...earn $1.37 in Boomerang Bucks
Availability: Available, ships in 8-11 days
Decoding Darkness by Rudolph E. Tanzi
Book DescriptionWorking from the intriguing hypothesis that Alzheimer's dementia is the result of a renegade protein-beta amyloid-Tanzi and others set out to find the gene responsible for its production. Decoding Darkness takes us deep into the minds and far-flung labs of many a prominent researcher, offering an intimate view of the high stakes of molecular genetics, the revolution that propels it, the obstacles that threaten to derail it, and the families whose lives are so dependent upon it. Tanzi and Parson ultimately reveal that Alzheimer's, like heart disease, may be effectively treated-even prevented.
Buy Decoding Darkness book by Rudolph E. Tanzi from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780738205267
(235mm x 156mm x 21mm)
Pages: 304
Imprint: Perseus Books
Publisher: The Perseus Books Group
Publish Date: 6-Dec-2001
Country of Publication: United States
Books By Author Rudolph E. Tanzi
Super Genes, Paperback (October 2016)
For years it was accepted knowledge that genes were fixed components of our bodies, and that we as individuals were incapable of altering our genetic make-up. Combining scientific research with insights from ancient traditions, the authors show how we need not be at the mercy of our genetic inheritance.
Super Brain, Paperback (August 2013)» View all books by Rudolph E. Tanzi
Shares an understanding of the brain and a prescriptive plan for how we can use it to achieve physical, mental and spiritual well-being. This title reveals 'the forgotten miracle' - the body's infinite capacity for change and renewal.
» Have you read this book? We'd like to know what you think about it - write a review about Decoding Darkness book by Rudolph E. Tanzi and you'll earn 50c in Boomerang Bucks loyalty dollars (you must be a member - it's free to sign up!)
Author Biography - Rudolph E. Tanzi
Rudolph E. Tanzi is Professor of Neurology at Harvard Medical School and Director of the Massachusetts General Hospital's Genetics and Aging Unit. Science journalist Ann B. Parson is co-author, with Isaac Schiff, M.D., of Menopause, and until recently taught in Boston University's graduate program in science and journalism.
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For every $20 you spend on books, you will receive $1 in Boomerang Bucks loyalty dollars. You can use your Boomerang Bucks as a credit towards a future purchase from Boomerang Books. Note that you must be a Member (free to sign up) and that conditions do apply.