text:
- Expert Pool Builders in Hammond, LA
- Professional Pool Builders in Hammond, LA
- Free Estimates with Pool Builders in Hammond, LA
Pool Builders in Hammond, LA
The pool builders Hammond, LA has to offer can provide you with the perfect way to relax and cool down on a hot summer day. You will find that Hammond pool builders will be able to build the exact type of pool you want, whether it is aboveground or underground. It is important that you take the time to think about the type of pool that you want, as there is a fairly big difference between the two. You will also need to consider how much space you have in back of your house for a new pool.
Avg Costs for Pool Builders
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$6,640.30 - $9,291.81
Find the Best Quality Pool Builders - Hammond, LA
The pool builder in Hammond, LA that you hire will be able to build the pool you want according to your exact specifications so you are not disappointed in the slightest. The pool builders in Hammond, LA specialize in doing this type of work so you won't have to do any of it yourself. A good pool builder will be able to construct the kind of pool you want so you and your family will have an excellent way to spend time together while cooling off in the warmer months of the year. There are pools available for everyone's budget, so you shouldn't have a problem affording one.
Pool Builders Companies
Tropical Pools
Hammond, LA 70401
Prime Cut Construction
11318 Highway 190 W
West, 11 70401
Mystic Pools, L.L.C.
47162 Oak Creek Trace
Hammond, LA 70401
H2O Pools LLC
Albany, LA 70711


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Nuts are a great way to add flavour and texture to meals, while also adding protein and healthy fatty acids. Try these recipes to put the crunch into your lunch!
Waldorf coleslaw
Mix together ½ a finely sliced white cabbage, 2 grated carrots, 2 chopped celery stalks, 2 tbs of golden raisins or sultanas and 2 tbs of plain peanuts. Chop an eating apple and toss in the juice of ½ a lemon and add to the salad. Stir in 1 tbs plain yoghurt and 1 tbs reduced fat mayonnaise, freshly ground black pepper and 1 tbs of freshly chopped parsley.
Makes 4 servings Nutrition information: 175 calories and 8g fat per serving.
Chicken cashew stir-fry
Heat 1 tsp sunflower oil in a non-stick frying pan or wok. Cut 2 small chicken fillets into diagonal strips and stir-fry over a high heat until cooked through. Add one sliced onion, ½ red and ½ green pepper, sliced, ½ inch of fresh ginger, peeled and grated and a crushed clove of garlic.
Cook for a few minutes. Stir in 2 tbs of oyster sauce, 2 tbs light soy sauce and the contents of a small tin of waterchestnuts, drained. Cook for another couple of minutes until heated through and top with 2 tbs of cashew nuts. Serve with noodles or rice.
Makes 2 servings. Nutrition information: 325 calories and 16g fat per serving.
Walnut Bread
In a large bowl, combine 300g strong flour and 200g wholemeal flour. Stir in a sachet of dried yeast, 75g of roughly chopped walnuts and 1 tsp walnut oil. Gradually add 300ml lukewarm water, stirring in with a knife and then using your hands as the dough comes together.
Flour a worktop generously, tip out the dough and knead gently for around 10 minutes or until the dough becomes more elastic. Return to a clean bowl, cover with a damp teatowl and leave to prove in a warm place for around an hour or until doubled in size. Turn the dough back on to the floured worktop and knead gently for another couple of minutes.
Place on a floured baking tray and bake at 190 degrees C for an hour or until the loaf sounds hollow when tapped underneath.
Makes 10 slices. Nutrition information: 230 calories and 6g fat per slice.
Start a diet plan at tescodiets.com
Thanks to tescodiets.com who have provided this article.

subset: commoncrawl


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According to the English media reports , Manchester United are after several top-quality and big name strikers , as the club's captain Wayne Rooney is still struggling with his poor form this season.
Reports claim that the Manchester United are desperately trying to sign Young Juventus starlet Paulo Dybala, who joined the Serie A champions from Palermo early on in the summer for a €32 million transfer fee.
Dyabla only just recently signed for the Bianconerri and there is no doubt that United will have to spend big to get Juventus to sell the young Argentina international.
United's main man has been out of form all season long bagging just a couple of goals on some occasions. LVG is keen on providing some added fire power to the United attacking third.
Louis Van Gaal is also reported to be after pacy wingers to improve United's goal scoring threat as United hasn't scored much goals this season.
Manchester United most recently recorded a home victory over West Brom last weekend with Juan Mata and Lingard getting on the scoresheet in that game.
This deal, if it happens, will surely frustrate Chelsea and Jose Mourinho as it was the Blues who were initially thought to be signing him as a replacement for Falcao who according to reports will be leaving Chelsea this summer.


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Best known as Guitar Guy from The Jeff Dunham Show, Brian Haner is that perfect combination of skilled guitar player and stand-up comedian. Brian was part of the largest comedy tour in the world while on the road with Jeff, seen live by over 2 million people. He was also part of the Jeff Dunham Christmas Special, the highest rated show in the history of Comedy Central, seen by 20 million people. While many guitar comics simply play chords while they do their comedy act, Brian is a whole different kind of musician. Brian spent twenty-five years as a guitarist, recording and touring with musicians ranging from Avenged Sevenfold to Frank Zappa. For the last four years he has opened for The Jeff Dunham Tour on more than 500 shows, in just about every city in America, as well as South Africa, Australia, and a tour across Europe. Brian is now headlining clubs across the country in his own rock 'n' roll comedy show. With over 5 million views on his YouTube page, Brian has created a rabid fanbase for his live shows.
Born in Youngstown, Ohio and raised Italian American and Catholic. My father was involved in a family business and my mother was/is an elementary school teacher. As a family, we moved back and forth between northeast Ohio and South Florida throughout my childhood. Started playing contact football at age 9 in Florida and wrestling at age 13 in Ohio. My senior year of high school I placed second in the Florida AAA State Tournament and went on to college at Pennsylvania State University where I wrestled in 1991-1992 and tutored student athletes. Moved to Philadelphia, Pennsylvania in 1995 with my bachelors degree in criminal justice. Began work counseling adjudicated teens and children with mental health issues. Started teaching special education in the Philadelphia School District in 1998. Received my masters in Special Education in 2001 from Cabrini College and continued to teach in both public school and residential school settings. Started stand up comedy in 2000 and moved to New York City in 2003. Was the 1983 and 1984 Spelling Bee runner up at Pine Grove Elementary School. I failed to advance to the national bee in Washington D.C. because of the complexity of the word roothless, which I still spell with two o's.
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My five must-have hair products!Thursday, January 23, 2014
Hask Placenta. Yep, you read that right. With a main ingredient like placenta, sheep's placenta to be exact, I can understand any hesitations you have with this product. I've been using it for years though and couldn't be happier with the results. I spray it on everyday before I brush my hair out after showers. It works wonders when my hair is less than manageable after bleaching and keeps my hair shiny, always.
2, 3 + 4 // Coconut oil, EVA NYC Therapy Session Hair Mask and Macadamia Oil Deep Repair Mask are three products I like to use before bleaching my hair (not at the same time, of course.) They each smell wonderful and leave my hair so much more smooth, shiny and manageable than before applying. Before bleaching your hair you want it to be in as good of shape as possible, using a hair mask will definitely help. Of course, after dying your hair you could use one as well but since they all need rinsed out in the end there is the chance of your color fading so I recommend using them often in the days leading up to the day you plan on bleaching.
5 // Boar hair brush. I picked up a boar hair brush at Target one day just because I needed a new brush. I got in the car, started brushing my hair and immediately realized this new brush was making my hair reeeeeal greasy. Come to find out, boar hair brushes are great for distributing your scalps natural oils to the rest of your hair. The thing is I have very fine hair so this brush makes my hair embarrassingly greasy. This was actually a really great discovery though. Now I always brush my hair thoroughly with this brush before bleaching my hair so the bleach does as little damage as possible. Your oils help protect your hair.
Other than those things I don't use much else. I use Sparks Color Care Sulfate-Free Shampoo + Conditioner which is awesome for helping maintain my hair color.
These products won't save super damaged hair and they're not miracle workers but they definitely help keep my hair super shiny and as healthy as possible. What are some of your must-have hair products? I love trying new hair masks!
25 thoughts
ok. I'm ready for a long shopping on line session.ReplyDelete
Hello! Another great product to use while bleaching is a protein filler. When you bleach you obviously take a lot of good stuff out of your hair. Sally's sells protein filler in four differenr tones (I use platinum). Mix your bleach, put half the bottle of filler in it, mix, & slap it on. It puts protein back into hair while also making the bleaching process produce more even results! (If you already do this, or have tried, I feel silly, but it couldn't hurt to share the info!)ReplyDelete
I bleach my roots every 3-4 weeks. I love It's a 10 Miracle leave in product, and Chi. They help protect hair from drying/tools.ReplyDelete
I use Dove Intensive repair conditioner and I swear it has improved my hair.
Ok, loved the Hask placenta one. Never heard about it and I'm gonna try it. I'm kinda on the phase I cut my hair now I want him to grow :)ReplyDelete
I have a hair mask from The Body Shop, but it's more lightweight moisture. I also got one from Lush as a present last year and it was really good. I don't know if you have Lush in the US. I use coconut oil for baking and in my homemade deodorant, but I haven't tried it on my hair yet.ReplyDelete
Thank you for sharing! I'll be bleaching my hair for the first time soon and love to hear what other people use for their hair. I'm using the Hask Henna-n-Placenta mask every so often leading up to it, and WOW. Y'all. Listen to her. Hask products are ah.ma.zing.ReplyDelete
Elyse @ Cuddly as a Cactus
Do you have to order the Hask Placenta from somewhere special or can you find it at a place like Sally Beauty Supply?ReplyDeleteReplies
You know, I'm not a 100% positive but I THINK I've seen it at Sally's. It'd never hurt to ask them when you're in there. :)Delete
I found that sulfate & paraben free shampoos didn't strip the color quite as fast as normal stuff. I use Whole Foods 365 brand since it's cheap. (Here a normal sized bottle is $3-4 and the gigantic bottle is $6)ReplyDelete
Thanks for the post! My hair needs some serious help and I plan on getting some coconut oil! ♥Replies
Oh, definitely! Sulfate is naaaasty for your hair, which is why it's so hilarious it's the first of second ingredient in most shampoos.Delete
I really need to get a hair mask. Thanks for sharing.ReplyDelete
Chrissy of crashbeauty.com
Thank you a ton for posting this!ReplyDelete
I'm in great need of new hair care products. c:
Thank you for posting this! I've gone platinum again and my hair is a fried mess.ReplyDelete
It makes me sad seeing all these cool things on Amazon when they won't ship any of them to Australia (apparently Priceline used to have the Hask and Seche Vite products, but I can't find them there any more - I can't seem to find anyone that will ship here!)ReplyDelete
I love the hair masks from TVAL.ReplyDelete
I don't hold them for the full 5 mins, but use them as conditioner twice a week (I dont user conditioner generally) and my hair is so soft. Didn't try their leave in shampoo or any of the solids though.
Yes, they sell hask placenta at Sally's. I just picked some up there. They only had small vials, though. Not the bigger bottles.ReplyDelete
I've heard that coconut oil is amazing in both your hair and body but I have only used it in my food. haha! Have you used Aussie's 3 minute miracle? If not I highly recommend it to you, it amazing!ReplyDelete
hello, i'm a silver haired girl.. my hair saviours are John Masters Organics products <3ReplyDelete
Thank you for posting this! I am looking into re-dying my hair soon to another funky color and this can help me better take care of it. Would you do a post on how to bleach your hair? I need more help in that department & I'm sure others do as well.ReplyDeleteReplies
I already did. There's actually a link in this post to that entry.Delete
- natalieFebruary 3, 2014 at 3:12 PM
Hi Kaylah have you ever tired putting coconut oil in your hair and then applying bleach over top. Its meant to protect the hair while its getting bleached but it doesnt affect the lightening of the hair...look it up :)ReplyDeleteReplies
I've heard of that but never tried it because what I do works insanely well for me.Delete
I must say, you certainly take care of your hair! I know lots of people who do terrible things to their hair! Coloring, heat, etc...and do nothing to protect it! Good job!ReplyDelete
How about a good dry shampoo?ReplyDeleteReplies
I've been using this recently, and loving it!Delete


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Rafal Maka
Rafal Maka is a professional in the game industry.
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ANG4043, a novel brain-penetrant peptide-mAb conjugate, is efficacious against HER2-positive intracranial tumors in mice
Demeule M.,AngioChem |
Tripathy S.,AngioChem |
Tripathy S.,Institute for Research in Immunology and Cancer |
And 6 more authors.
Molecular Cancer Therapeutics | Year: 2015
Anti-HER2 monoclonal antibodies (mAb) have been shown to reduce tumor size and increase survival in patients with breast cancer, but they are ineffective against brain metastases due to poor brain penetration. In previous studies, we identified a peptide, known as Angiopep-2 (An2), which crosses the blood-brain barrier (BBB) efficiently via receptor-mediated transcytosis, and, when conjugated, endows small molecules and peptides with this property. Extending this strategy to higher molecular weight biologics, we now demonstrate that a conjugate between An2 and an anti-HER2 mAb results in a new chemical entity, ANG4043, which retains in vitro binding af finity for the HER2 receptor and antiproliferative potency against HER2-positive BT-474 breast ductal carcinoma cells. Unlike the native mAb, ANG4043 binds LRP1 clusters and is taken up by LRP1-expressing cells. Measuring brain exposure after intracarotid delivery, we demonstrate that the new An2-mAb conjugate penetrates the BBB with a rate of brain entry (Kin) of 1.6 x 10-3 mL/g/s. Finally, in mice with intracranially implanted BT-474 xenografts, systemically administered ANG4043 increases survival. Overall, this study demonstrates that the incorporation of An2 to the anti-HER2 mAb confers properties of increased uptake in brain endothelial cells as well as BBB permeability. These characteristics of ANG4043 result in higher exposure levels in BT-474 brain tumors and prolonged survival following systemic treatment. Moreover, the data further validate the An2-drug conjugation strategy as a way to create brain-penetrant biologics for neuro-oncology and other CNS indications. © 2014 American Association for Cancer Research. Source
Lacombe J.,Institute for Research in Immunology and Cancer
Blood | Year: 2013
SCL/TAL1, a tissue-specific transcription factor of the basic helix-loop-helix family, and c-Kit, a tyrosine kinase receptor, control hematopoietic stem cell survival and quiescence. Here we report that SCL levels are limiting for the clonal expansion of Kit+ multipotent and erythroid progenitors. In addition, increased SCL expression specifically enhances the sensitivity of these progenitors to steel factor (KIT ligand) without affecting interleukin-3 response, whereas a DNA-binding mutant antagonizes KIT function and induces apoptosis in progenitors. Furthermore, a twofold increase in SCL levels in mice bearing a hypomorphic Kit allele (W41/41) corrects their hematocrits and deficiencies in erythroid progenitor numbers. At the molecular level, we found that SCL and c-Kit signaling control a common gene expression signature, of which 19 genes are associated with apoptosis. Half of those were decreased in purified megakaryocyte/erythroid progenitors (MEPs) from W41/41 mice and rescued by the SCL transgene. We conclude that Scl operates downstream of Kit to support the survival of MEPs. Finally, higher SCL expression upregulates Kit in normal bone marrow cells and increases chimerism after bone marrow transplantation, indicating that Scl is also upstream of Kit. We conclude that Scl and Kit establish a positive feedback loop in multipotent and MEPs. Source
News Article
Site: http://www.nature.com/nature/current_issue/
NOD/SCID Il2rgnull mice (Jackson Laboratory) were bred and maintained in the Stem Cell Unit animal barrier facility at McMaster University. All procedures were approved by the Animal Research Ethics Board at McMaster University. All patient samples were obtained with informed consent and with the approval of local human subject research ethics boards at McMaster University. Human umbilical cord blood mononuclear cells were collected by centrifugation with Ficoll-Paque Plus (GE), followed by red blood cell lysis with ammonium chloride (StemCell Technologies). Cells were then incubated with a cocktail of lineage-specific antibodies (CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; StemCell Technologies) for negative selection of Lin− cells using an EasySep immunomagnetic column (StemCell Technologies). Live cells were discriminated on the basis of cell size, granularity and, as needed, absence of viability dye 7-AAD (BD Biosciences) uptake. All flow cytometry analysis was performed using a BD LSR II instrument (BD Biosciences). Data acquisition was conducted using BD FACSDiva software (BD Biosciences) and analysis was performed using FlowJo software (Tree Star). To quantify MSI2 expression in human HSPCs, Lin− cord blood cells were stained with the appropriate antibody combinations to resolve HSC (CD34+ CD38− CD45RA− CD90+), MPP (CD34+ CD38− CD45RA− CD90−), CMP (CD34+ CD38+ CD71−) and EP (CD34+ CD38+ CD71+) fractions as similarly described previously18, 19 with all antibodies from BD Biosciences: CD45RA (HI100), CD90 (5E10), CD34 (581), CD38 (HB7) and CD71 (M-A712). Cell viability was assessed using the viability dye 7AAD (BD Biosciences). All cell subsets were isolated using a BD FACSAria II cell sorter (BD Biosciences) or a MoFlo XDP cell sorter (Beckman Coulter). HemaExplorer20 analysis was used to confirm MSI2 expression in human HSPCs and across the hierarchy. For all qRT–PCR determinations total cellular RNA was isolated with TRIzol LS reagent according to the manufacturer's instructions (Invitrogen) and cDNA was synthesized using the qScript cDNA Synthesis Kit (Quanta Biosciences). qRT–PCR was done in triplicate with PerfeCTa qPCR SuperMix Low ROX (Quanta Biosciences) with gene-specific probes (Universal Probe Library (UPL), Roche) and primers: MSI2 UPL-26, F-GGCAGCAAGAGGATCAGG, R-CCGTAGAGATCGGCGACA; HSP90 UPL-46, F-GGGCAACACCTCTACAAGGA, R-CTTGGGTCTGGGTTTCCTC; CYP1B1 UPL-20, F-ACGTACCGGCCACTATCACT, R-CTCGAGTCTGCACATCAGGA; GAPDH UPL-60, F-AGCCACATCGCTCAGACAC, R-GCCCAATACGACCAAATCC; ACTB (UPL Set Reference Gene Assays, Roche). The mRNA content of samples compared by qRT–PCR was normalized based on the amplification of GAPDH or ACTB. MSI2 shRNAs were designed with the Dharmacon algorithm (http://www.dharmacon.com). Predicted sequences were synthesized as complimentary oligonucleotides, annealed and cloned downstream of the H1 promoter of the modfied cppt-PGK-EGFP-IRES-PAC-WPRE lentiviral expression vector18. Sequences for the MSI2 targeting and control RFP targeting shRNAs were as follows: shMSI2, 5′-GAGAGATCCCACTACGAAA-3′; shRFP, 5′-GTGGGAGCGCGTGATGAAC-3′. Human MSI2 cDNA (BC001526; Open Biosystems) was subcloned into the MA bi-directional lentiviral expression vector21. Human CYP1B1 cDNA (BC012049; Open Biosystems) was cloned in to psMALB22. All lentiviruses were prepared by transient transfection of 293FT (Invitrogen) cells with pMD2.G and psPAX2 packaging plasmids (Addgene) to create VSV-G pseudotyped lentiviral particles. All viral preparations were titrated on HeLa cells before use on cord blood. Standard SDS–PAGE and western blotting procedures were performed to validate the effects of knockdown on transduced NB4 cells (DSMZ) and overexpression on 293FT cells. Immunoblotting was performed with anti-MSI2 rabbit monoclonal IgG (EP1305Y, Epitomics) and β-actin mouse monoclonal IgG (ACTBD11B7, Santa Cruz Biotechnology) antibodies. Secondary antibodies used were IRDye 680 goat anti-rabbit IgG and IRDye 800 goat anti-mouse IgG (LI-COR). 293FT and NB4 cell lines tested negative for mycoplasma. NB4 cells were authenticated by ATRA treatment before use. Cord blood transductions were conducted as described previously18, 23. Briefly, thawed Lin− cord blood or flow-sorted Lin− CD34+ CD38− or Lin− CD34+ CD38+ cells were prestimulated for 8–12 h in StemSpan medium (StemCell Technologies) supplemented with growth factors interleukin 6 (IL-6; 20 ng ml−1, Peprotech), stem cell factor (SCF; 100 ng ml−1, R&D Systems), Flt3 ligand (FLT3-L; 100 ng ml−1, R&D Systems) and thrombopoietin (TPO; 20 ng ml−1, Peprotech). Lentivirus was then added in the same medium at a multiplicity of infection of 30–100 for 24 h. Cells were then given 2 days after transduction before use in in vitro or in vivo assays. For in vitro cord blood studies biological (experimental) replicates were performed with three independent cord blood samples. Human clonogenic progenitor cell assays were done in semi-solid methylcellulose medium (Methocult H4434; StemCell Technologies) with flow-sorted GFP+ cells post transduction (500 cells per ml) or from day seven cultured transduced cells (12,000 cells per ml). Colony counts were carried out after 14 days of incubation. CFU-GEMMs can seed secondary colonies owing to their limited self-renewal potential24. Replating of MSI2-overexpressing and control CFU-GEMMs for secondary CFU analysis was performed by picking single CFU-GEMMs at day 14 and disassociating colonies by vortexing. Cells were spun and resuspended in fresh methocult, mixed with a blunt-ended needle and syringe, and then plated into single wells of a 24-well plate. Secondary CFU analysis for shMSI2- and shControl-expressing cells was performed by harvesting total colony growth from a single dish (as nearly equivalent numbers of CFU-GEMMs were present in each dish), resuspending cells in fresh methocult by mixing vigorously with a blunt-ended needle and syringe and then plating into replicate 35-mm tissue culture dishes. In both protocols, secondary colony counts were done following incubation for 10 days. For primary and secondary colony forming assays performed with the AHR agonist FICZ (Santa Cruz Biotechnology), 200 nM FICZ or 0.1% DMSO was added directly to H4434 methocult medium. Two-way ANOVA analysis was performed to compare secondary CFU output and FICZ treatment for MSI2-overexpressing or control conditions. Colonies were imaged with a Q-Colour3 digital camera (Olympus) mounted to an Olympus IX5 microscope with a 10× objective lens. Image-Pro Plus imaging software (Media Cybernetics) was used to acquire pictures and subsequent image processing was performed with ImageJ software (NIH). Transduced human Lin− cord blood cells were sorted for GFP expression and seeded at a density of 105 cells per ml in IMDM 10% FBS supplemented with human growth factors IL-6 (10 ng ml−1), SCF (50 ng ml−1), FLT3-L (50 ng ml−1), and TPO (20 ng ml−1) as previously described25. To generate growth curves, every seven days cells were counted, washed, and resuspended in fresh medium with growth factors at a density of 105 cells per ml. Cells from suspension cultures were also used in clonogenic progenitor, cell cycle and apoptosis assays. Experiments performed on transduced Lin− CD34+ cord blood cells used serum-free conditions as described in the cord blood transduction subsection of Methods. For in vitro cord blood studies, biological (experimental) replicates were performed with three independent cord blood samples. Cell cycle progression was monitored with the addition of BrdU to day 10 suspension cultures at a final concentration of 10 μM. After 3 h of incubation, cells were assayed with the BrdU Flow Kit (BD Biosciences) according to the manufacturer's protocol. Cell proliferation and quiescence were measured using Ki67 (BD Bioscience) and Hoechst 33342 (Sigma) on day 4 suspension cultures after fixing and permeabilizing cells with the Cytofix/Cytoperm kit (BD Biosciences). For apoptosis analysis, Annexin V (Invitrogen) and 7-AAD (BD Bioscience) staining of day 7 suspension cultures was performed according to the manufacturer's protocol. Lin− cord blood cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions. Fixed and permeabilized cells were immunostained with anti-MSI2 rabbit monoclonal IgG antibody (EP1305Y, Abcam) and detected by Alexa-488 goat anti-rabbit IgG antibody (Invitrogen). CD34+ cells were transduced with an MSI2-overexpression or MSI2-knockdown lentivirus along with their corresponding controls and sorted for GFP expression 3 days later. Transductions for MSI2 overexpression or knockdown were each performed on two independent cord blood samples. Total RNA from transduced cells (>1 × 105) was isolated using TRIzol LS as recommended by the manufacturer (Invitrogen), and then further purified using RNeasy columns (Qiagen). Sample quality was assessed using Bioanalyzer RNA Nano chips (Agilent). Paired-end, barcoded RNA-seq sequencing libraries were then generated using the TruSeq RNA Sample Prep Kit (v2) (Illumina) following the manufacturer's protocols starting from 1 μg total RNA. The quality of library generation was then assessed using a Bioanalyzer platform (Agilent) and Illumina MiSeq-QC run was performed or quantified by qPCR using KAPA quantification kit (KAPA Biosystems). Sequencing was performed using an Illumina HiSeq2000 using TruSeq SBS v3 chemistry at the Institute for Research in Immunology and Cancer's Genomics Platform (University of Montreal) with cluster density targeted at 750,000 clusters per mm2 and paired-end 2 × 100-bp read lengths. For each sample, 90–95 million reads were produced and mapped to the hg19 (GRCh37) human genome assembly using CASAVA (version 1.8). Read counts generated by CASAVA were processed in EdgeR (edgeR_3.12.0, R 3.2.2) using TMM normalization, paired design, and estimation of differential expression using a generalized linear model (glmFit). The false discovery rate (FDR) was calculated from the output P values using the Benjamini–Hochberg method. The fold change of logarithm of base 2 of TMM normalized data (logFC) was used to rank the data from top upregulated to top downregulated genes and FDR (0.05) was used to define significantly differentially expressed genes. RNA-seq data have been deposited in NCBI's Gene Expression Omnibus (GEO) and are accessible through GEO Series accession number GSE70685. iRegulon26 was used to retrieve the top 100 AHR predicted targets with a minimal occurrence count threshold of 5. The data were analysed using GSEA27 with ranked data as input with parameters set to 2,000 gene-set permutations. The GEO dataset GSE28359, which contains Affymetrix Human Genome U133 Plus 2.0 Array gene expression data for CD34+ cells treated with SR1 at 30 nM, 100 nM, 300 nM and 1,000 nM was used to obtain lists of genes differentially expressed in the treated samples compared to the control ones (0 nM)2. Data were background corrected using Robust Multi-Array Average (RMA) and quantile normalized using the expresso() function of the affy Bioconductor package (affy_1.38.1, R 3.0.1). Lists of genes were created from the 150 top upregulated and downregulated genes from the SR1-treated samples at each dose compared to the non-treated samples (0 nM). The data were analysed using GSEA with ranked data as input with parameters set to 2,000 gene-set permutations. The normalized enrichment score (NES) and false discovery rate (FDR) were calculated for each comparison. The GEO data set GSE24759, which contains Affymetrix GeneChip HT-HG_U133A Early Access Array gene expression data for 38 distinct haematopoietic cell states4, was compared to the MSI2 overexpression and knockdown data. GSE24759 data were background corrected using Robust Multi-Array Average (RMA), quantile normalized using the expresso() function of the affy Bioconductor package (affy_1.38.1, R 3.0.1), batch corrected using the ComBat() function of the sva package (sva_3.6.0) and scaled using the standard score. Bar graphs were created by calculating for significantly differentially expressed genes the number of scaled data that were above (>0) or below (<0) the mean for each population. Percentages indicating for how long the observed value (set of up- or downregulated genes) was better represented in that population than random values were calculated from 1,000 trials. A unique list of genes closest to AHR-bound regions previously identified from TCDD-treated MCF7 ChIP–seq data14 was used to calculate the overlap with genes showing >1.5-fold downregulation in response to treatment with UM171 (35 nM) or SR1 (500 nM) relative to DMSO-treated samples3 as well as with genes significantly downregulated in MSI2-overexpressing versus control treated samples (FDR < 0.05). The percentage of downregulated genes with AHR-bound regions was then plotted for each gene set. P values were generated with Fisher's exact test for comparisons between gene lists. AHR transcription factor binding sites in downregulated gene sets were identified with oPOSSUM-328. Genes showing >1.5-fold downregulation in response to treatment with UM171 (35 nM) or SR1 (500 nM) relative to DMSO-treated samples3 were used along with significantly downregulated genes (FDR < 0.05) with EdgeR-analysed MSI2-overexpressing versus control-treated samples. The three gene lists were uploaded into oPOSSUM-3 and the AHR:ARNT transcription factor binding site profile was used with the matrix score threshold set at 80% to analyse the region 1,500 bp upstream and 1,000 bp downstream of the transcription start site. The percentage of downregulated genes with AHR-binding sites in their promoters was then plotted for each gene set. Fisher's exact test was used to identify significant overrepresentation of AHR-binding sites in gene lists relative to background. Eight- to 12-week-old male or female NSG mice were sublethally irradiated (315 cGy) one day before intrafemoral injection with transduced cells carried in IMDM 1% FBS at 25 μl per mouse. Injected mice were analysed for human haematopoietic engraftment 12–14 weeks after transplantation or at 3 and 6.5 weeks for STRC experiments. Mouse bones (femurs, tibiae and pelvis) and spleen were removed and bones were crushed with a mortar and pestle then filtered into single-cell suspensions. Bone marrow and spleen cells were blocked with mouse Fc block (BD Biosciences) and human IgG (Sigma) and then stained with fluorochrome-conjugated antibodies specific to human haematopoietic cells. For multilineage engraftment analysis, cells from mice were stained with CD45 (HI30) (Invitrogen), CD33 (P67.6), CD15 (HI98), CD14 (MφP9), CD19 (HIB19), CD235a/GlyA (GA-R2), CD41a (HIP8) and CD34 (581) (BD Biosciences). For MSI2 knockdown in HSCs, 5.0 × 104 and 2.5 × 104 sorted Lin− CD34+ CD38− cells were used per short-hairpin transduction experiment, leading to transplantation of day zero equivalent cell doses of 10 × 103 and 6.25 × 103, respectively, per mouse. For STRC LDA transplantation experiments, 105 sorted CD34+CD38+ cells were used per control or MSI2-overexpressing transduction. After assessing levels of gene transfer, day zero equivalent GFP+ cell doses were calculated to perform the LDA. Recipients with greater than 0.1% GFP+CD45+/− cells were considered to be repopulated. For STRC experiments that read out extended engraftment at 6.5 weeks, 2 × 105 CD34+ CD38+ cells were used per overexpressing or control transduction to allow non-limiting 5 × 104 day zero equivalent cell doses per mouse. For HSC expansion and LDA experiments, CD34+CD38− cells were sorted and transduced with MSI2-overexpressing or control vectors (50,000 cells per condition) for 3 days and then analysed for gene-transfer levels (% GFP+/−) and primitive cell marker expression (% CD34 and CD133). To ensure that equal numbers of GFP+ cells were transplanted into both control and MSI2-overexpressing recipient mice, we added identically cultured GFP− cells to the MSI2 culture to match the % GFP+ of the control culture (necessary owing to the differing efficiency of transduction). The adjusted MSI2-overexpressing culture was recounted and aliquoted (63,000 cells) to match the output of half of the control culture. Three day 0 equivalent GFP+ cell doses (1,000, 300 and 62 cells) were then transplanted per mouse to perform the D3 primary LDA. A second aliquot of the adjusted MSI2-overexpressing culture was then taken and put into culture in parallel with the remaining half of the control culture to perform another LDA after 7 days of growth (10 days total growth, D10 primary LDA). Altogether, four cell doses were transplanted; when converted back to day 0 equivalents these equalled approximately 1,000, 250, 100, and 20 GFP+ cells per mouse, respectively. Pooled bone marrow from six engrafted primary mice that received D10 cultured control or MSI2-overexpressing cells (from the two highest doses transplanted) was aliquoted into five cell doses of 15 million, 10 million, 6 million, 2 million and 1 million cells. The numbers of GFP+ cells within primary mice was estimated from nucleated cell counts obtained from NSG femurs, tibias and pelvises and from Colvin et al.29. The actual numbers of GFP+ cells used for determining numbers of GFP+ HSCs and the number of mice transplanted for all LDA experiments is shown in Supplementary Tables 3–5. The cut-off for HSC engraftment was a demonstration of multilineage reconstitution that was set at bone marrow having >0.1% GFP+ CD33+ and >0.1% GFP+ CD19+ cells. HSC and STRC frequency was assessed using ELDA software30. For all mouse transplantation experiments, mice were age- (6–12 week) and sex-matched. All transplanted mice were included for analysis unless mice died from radiation sickness before the experimental endpoint. No randomization or blinding was performed for animal experiments. Approximately 3–6 mice were used per cell dose for each cord blood transduction and transplantation experiment. CLIP–seq was performed as previously described15. Briefly, 25 million NB4 cells (a transformed human cell line of haematopoietic origin) were washed in PBS and UV-cross-linked at 400 mJ cm−2 on ice. Cells were pelleted, lysed in wash buffer (PBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40) and DNase-treated, and supernatants from lysates were collected for immunoprecipitation. MSI2 was immunoprecipitated overnight using 5 μg of anti-MSI2 antibody (EP1305Y, Abcam) and Protein A Dynabeads (Invitrogen). Beads containing immunoprecipated RNA were washed twice with wash buffer, high-salt wash buffer (5× PBS, 0.1% SDS, 0.5% Na-Deoxycholate, 0.5% NP-40), and PNK buffer (50 mM Tris-Cl pH 7.4, 10 mM MgCl , 0.5% NP-40). Samples were then treated with 0.2 U MNase for 5 min at 37° with shaking to trim immunopreciptated RNA. MNase inactivation was then carried out with PNK + EGTA buffer (50 mM Tris-Cl pH 7.4, 20 mM EGTA, 0.5% NP-40). The sample was dephosphorylated using alkaline phosphatase (CIP, NEB) at 37° for 10 min followed by washing with PNK+EGTA, PNK buffer, and then 0.1 mg ml−1 BSA in nuclease-free water. 3′RNA linker ligation was performed at 16° overnight with the following adaptor: 5′P-UGGAAUUCUCGGGUGCCAAGG-puromycin. Samples were then washed with PNK buffer, radiolabelled using P32-y-ATP (Perkin Elmer), run on a 4–12% Bis-Tris gel and then transferred to a nitrocellulose membrane. The nitrocellulose membrane was developed via autoradiography and RNA–protein complexes 15–20 kDa above the molecular weight of MSI2 were extracted with proteinase K followed by RNA extraction with acid phenol-chloroform. A 5′RNA linker (5′HO-GUUCAGAGUUCUACAGUCCGACGAUC-OH) was ligated to the extracted RNA using T4 RNA ligase (Fermentas) for 2 h and the RNA was again purified using acid phenol-chloroform. Adaptor ligated RNA was re-suspended in nuclease-free water and reverse transcribed using Superscript III reverse transcriptase (Invitrogen). Twenty cycles of PCR were performed using NEB Phusion Polymerase using a 3′PCR primer that contained a unique Illumina barcode sequence. PCR products were run on an 8% TBE gel. Products ranging between 150 and 200 bp were extracted using the QIAquick gel extraction kit (Qiagen) and re-suspended in nuclease-free water. Two separate libraries were prepared and sent for single-end 50-bp Illumina sequencing at the Institute for Genomic Medicine at the University of California, San Diego. 47,098,127 reads from the first library passed quality filtering, of which 73.83% mapped uniquely to the human genome. 57,970,220 reads from the second library passed quality filtering, of which 69.53% mapped uniquely to the human genome. CLIP-data reproducibility was verified through high correlation between gene RPKMs and statistically significant overlaps in the clusters and genes within replicates. CLIP–seq data have been deposited in NCBI's GEO and are accessible through GEO Series accession number GSE69583. Before sequence alignment of CLIP–seq reads to the human genome was performed, sequencing reads from libraries were trimmed of polyA tails, adapters, and low quality ends using Cutadapt with parameters–match-read-wildcards–times 2 -e 0 -O 5–quality-cutoff' 6 -m 18 -b TCGTATGCCGTCTTCTGCTTG -b ATCTCGTATGCCGTCTTCTGCTTG -b CGACAGGTTCAGAGTTCTACAGTCCGACGATC -b TGGAATTCTCGGGTGCCAAGG -b AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-b TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT. Reads were then mapped against a database of repetitive elements derived from RepBase (version 18.05). Bowtie (version 1.0.0) with parameters -S -q -p 16 -e 100 -l 20 was used to align reads against an index generated from Repbase sequences31. Reads not mapped to Repbase sequences were aligned to the hg19 human genome (UCSC assembly) using STAR (version 2.3.0e)32 with parameters–outSAMunmapped Within –outFilterMultimapNmax 1 –outFilterMultimapScoreRange 1. To identify clusters in the genome of significantly enriched CLIP–seq reads, reads that were PCR replicates were removed from each CLIP–seq library using a custom script of the same method as in ref. 33; otherwise, reads were kept at each nucleotide position when more than one read's 5′-end was mapped. Clusters were then assigned using the CLIPper software with parameters–bonferroni–superlocal–threshold-34. The ranked list of significant targets was calculated assuming a Poisson distribution, where the observed value is the number of reads in the cluster, and the background is the number of reads across the entire transcript and or across a window of 1000 bp ± the predicted cluster. Transcriptomic regions and gene classes were defined using annotations found in gencode v17. Depending on the analysis, clusters were associated by the Gencode-annotated 5′UTR, 3′UTR, CDS or intronic regions. If a cluster overlapped multiple regions, or a single part of a transcript was annotated as multiple regions, clusters were iteratively assigned first as CDS, then 3′UTR, 5′UTR and finally as proximal (<500 bases from an exon) or distal (>500 bases from an exon) introns. Overlapping peaks were calculated using bedtools and pybedtools35, 36. Significantly enriched gene ontology (GO) terms were identified using a hypergeometric test that compared the number of genes that were MSI2 targets in each GO term to genes expressed in each GO term as the proper background. Expressed genes were identified using the control samples in SRA study SRP012062. Mapping was performed identically to CLIP–seq mapping, without peak calling and changing the STAR parameter outFilterMultimapNmax to 10. Counts were calculated with featureCounts37 and RPKMs were then computed. Only genes with a mean RPKM > 1 between the two samples were used in the background expressed set. Randomly located clusters within the same genic regions as predicted MSI2 clusters were used to calculate a background distribution for motif and conservation analyses. Motif analysis was performed using the HOMER algorithm as in ref. 34. For evolutionary sequence conservation analysis, the mean (mammalian) phastCons score for each cluster was used. CD34+ cells (>5 × 104) were transduced with an MSI2-overexpression or control lentivirus. Three days later, GFP+ cells were sorted and then put back in to StemSpan medium containing growth factors IL-6 (20 ng ml−1), SCF (100 ng ml−1), FLT3-L (100 ng ml−1) and TPO (20 ng ml−1). A minimum of 10,000 cells were used for immunostaining at culture days 3 and 7 after GFP sorting. Cells were fixed in 2% PFA for 10 min, washed with PBS and then cytospun on to glass slides. Cytospun cells were then permeabilized (PBS, 0.2% Triton X-100) for 20 min, blocked (PBS, 0.1% saponin, 10% donkey serum) for 30 min and stained with primary antibodies (CYP1B1 (EPR14972, Abcam); HSP90 (68/hsp90, BD Biosciences)) in PBS with 10% donkey serum for 1 h. Detection with secondary antibody was performed in PBS 10% donkey serum with Alexa-647 donkey anti-rabbit antibody or Alexa-647 donkey anti-mouse antibodies for 45 min. Slides were mounted with Prolong Gold Antifade containing DAPI (Invitrogen). Several images (200–1,000 cells total) were captured per slide at 20× magnification using an Operetta HCS Reader (Perkin Elmer) with epifluorescence illumination and standard filter sets. Columbus software (Perkin Elmer) was used to automate the identification of nuclei and cytoplasm boundaries in order to quantify mean cell fluorescence. A 271-bp region of the CYP1B1 3′UTR that flanked CLIP–seq-identified MSI2-binding sites was cloned from human HEK293FT genomic DNA using the forward primer GTGACACAACTGTGTGATTAAAAGG and reverse primer TGATTTTTATTATTTTGGT AATGGTG and placed downstream of renilla luciferase in the dual-luciferase reporter vector pGL4 (Promega). A 271-bp geneblock (IDT) with 6 TAG > TCC mutations was cloned in to pGL4 using XbaI and NotI. The HSP90 3′UTR was amplified from HEK293FT genomic DNA with the forward primer TCTCTGGCTGAGGGATGACT and reverse primer TTTTAAGGCCAAGGAATTAAGTGA and cloned into pGL4. A geneblock of the HSP90 3′UTR (IDT) with 14 TAG > TCC mutations was cloned in to pGL4 using SfaAI and NotI. Co-transfection of wild-type or mutant luciferase reporter (40 ng) and control or MSI2-overexpressing lentiviral expression vector (100 ng) was performed in the NIH-3T3 cell line, which does not express MSI1 or MSI2 (50,000 cells per co-transfection). Reporter activity was measured using the Dual-Luciferase Reporter Assay System (Promega) 36–40 h later. For MSI2-overexpressing cultures with the AHR antagonist SR1, Lin− CD34+ cells were transduced with MSI2-overexpression or control lentivirus in medium supplemented with SR1 (750 nM; Abcam) or DMSO vehicle (0.1%). GFP+ cells were isolated (20,000 cells per culture) and allowed to proliferate with or without SR1 for an additional 7 days at which point they were counted and immunophenotyped for CD34 and CD133 expression. For MSI2-overexpressing cultures with the AHR agonist FICZ, Lin− CD34+ cells were transduced with MSI2-overexpression or control lentivirus. GFP+ cells were isolated (20,000 cells per culture) and allowed to proliferate with FICZ (200 nM; Santa Cruz Biotechnology) or DMSO (0.1%) for an additional 3 days, at which point they were immunophenotyped for CD34 and CD133 expression. Lin− CD34+ cells were cultured for 72 h (lentiviral treated but non-transduced flow-sorted GFP− cells) in StemSpan medium containing growth factors IL-6 (20 ng ml−1), SCF (100 ng ml−1), FLT3-L (100 ng ml−1) and TPO (20 ng ml−1) before the addition of the CYP1B1 inhibitor TMS (Abcam) at a concentration of 10 μM or mock treatment with 0.1% DMSO. Equal numbers of cells (12,000 per condition) were then allowed to proliferate for 7 days at which point they were counted and immunophenotyped for CD34 and CD133 expression. Unless stated otherwise (that is, analysis of RNA–seq and CLIP–seq data sets), all statistical analysis was performed using GraphPad Prism (GraphPad Software version 5.0). Unpaired student t-tests or Mann–Whitney tests were performed with P < 0.05 as the cut-off for statistical significance. No statistical methods were used to predetermine sample size.
Vincent K.,Institute for Research in Immunology and Cancer |
Vincent K.,University of Montreal |
Roy D.-C.,University of Montreal |
Perreault C.,Institute for Research in Immunology and Cancer |
Perreault C.,University of Montreal
Blood | Year: 2011
Allogeneic hematopoietic cell transplantation led to the discovery of the allogeneic GVL effect, which remains the most convincing evidence that immune cells can cure cancer in humans. However, despite its great paradigmatic and clinical relevance, induction of GVL by conventional allogeneic hematopoietic cell transplantation remains a quite rudimentary form of leukemia immunotherapy. It is toxic and its efficacy is far from optimal. It is therefore sobering that since the discovery of the GVL effect 3 decades ago, the way GVL is induced and manipulated has practically not changed. Preclinical and clinical studies suggest that injection of T cells primed against a single Ag present on neoplastic cells could enhance the GVL effect without causing any GVHD. We therefore contend that Ag-targeted adoptive T-cell immunotherapy represents the future of leukemia immunotherapy, and we discuss the specific strategies that ought to be evaluated to reach this goal. Differences between these strategies hinge on 2 key elements: the nature of the target Ag and the type of Ag receptor expressed on T cells. © 2011 by The American Society of Hematology. Source
Piekny A.J.,Concordia University at Montreal |
Maddox A.S.,Institute for Research in Immunology and Cancer
Seminars in Cell and Developmental Biology | Year: 2010
Anillin is a highly conserved multidomain protein that interacts with cytoskeletal components as well as their regulators. Throughout phylogeny, Anillins contribute to cytokinesis, the cell shape change that occurs at the end of meiosis and mitosis to separate a cell into daughter cells. Failed cytokinesis results in binucleation, which can lead to genomic instability. Study of Anillin in several model organisms has provided us with insight into how the cytoskeleton is coordinated to ensure that cytokinesis occurs with high fidelity. Here we review Anillin's interacting partners and the relevance of these interactions in vivo. We also discuss questions of how these interactions are coordinated, and finally provide some perspective regarding Anillin's role in cancer. © 2010 Elsevier Ltd. Source


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Quantum computing: achievable reality or unrealistic dream
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Today, really desperate to get a job, I filled out an application for a dishwashing job. My application got tossed out, because I'm not an economics major like the other guy applying for the same job. FML
By Anonymous - / Sunday 10 February 2013 17:55 / Norway - Bergen
Top comments
By 0RedPenguin0
I know that going to college can help you get a job, but I didn't realize that economics were a part of washing dishes
What is this world coming to?
By 0RedPenguin0
I know that going to college can help you get a job, but I didn't realize that economics were a part of washing dishes
Too many negative votes, comment buried. Show the comment
My guess would be maybe OP doesn't have a degree? But then they should higher her because they could pay her less
Most employers will pick someone with any 4 year degree over someone without a degree at all.
They're usually more willing to pay more for someone with a degree.
Degree show you can finish what you start
Home economics!
Anyone who wants to try out a awesome free game, type in league of legends download it and sign up. Btw when it asks for someone who reccommended you copy paste this name: MasturbatingB3ar
29, not when you're applying for minimum wage jobs-- the higher the degree, the better chances of you being hired. I know this because many of English major friends are working minimum wage jobs (mostly baristas) and the only reason they got those jobs was because they had a BA as opposed to GED/high school diploma/Asssociate's that others had. I was an English major too but I did a double major in Biology, as well. I'm pretty sure a dishwashing job wouldn't pay any more than minimum wage. Pretty much any job that is based on hourly pay (and not salary) would prefer to hire someone with a BS/BA over anything less. The way I see it: even a BA in Business in better than an associates in biochem (if that's even a thing?) simply because I've never come across a job that wants to hire someone with an associate's; they require either high school grad or a college grad. Like my company's HR head said, "Associate's might as well be renamed to 'college dropout' degree unless you actually go to college after getting one."
47 - Associates are more for vocational schools. Careers such as lab techs, RNs, CT/x-ray techs, and ultrasound techs are often only offered associates degrees, with continued education as they progress in their field. The degrees are mostly career-specific and are usually programs that have combined externships for the field.
I was mostly referring to the people who get an associate's degree and think they're all done with school. I suppose I struck a nerve there with some of them with that last comment. I just think it's worthless to go to a CC and get one, unless you're actually doing something else after it. Like enrolling in a college or nursing school. A good four-year college education is absolutely essential to succeed in today's world. I'm thankful to be in med school but I can't imagine having to try to find a job with just an associate's.
That's because of hipsters and their parent's money.
Except wet noodles.
Somebody killed a good thread^^^
Yup, My toe is whore, for it also bangs everything. So I really know the feeling.
To be a barista all you need is an art degree.
No, it really is F OP's life.
At least he got the job
By GingerNinja7
Think of it this way, at least you didn't spend thousands of dollars for schooling only to find work as a dish washer
So not true. People with degrees CAN go places, just depends how hard they want to work to get there. Don't go convincing kids that university is a waste of time... Broaden your social network OP! The more people professional you know, the easier getting a (good) job will be!
Hard work? More like start kissing major @$$.
Um, excuse me, I have two undergraduate degrees, and am getting my third this year, thank you. I'm currently a barista. Don't go shooting your mouth off about something I never said. I don't discourage anyone from getting a higher education. I'm just saying facts are facts. It's not about what you know these days, but who. College graduates are still having the hardest time finding work post graduation, which is why I went back so I could do exactly what you're recommending, broadening my social network and making contacts.
You said 'college degrees get you nowhere.' That's just not true.
I know my own friends don't constitute a viable data pool, but I have many many friends and acquaintances who have graduated, some bilingual with master's degrees, some with bachelor's degrees, and maybe one or two are actually doing anything pertaining to their field for a livable wage. It's in the newspapers all the time that college degrees aren't getting anyone as far as they used to. Recently I read an article that used a young man who graduated two years ago with the same degree I have and is still working at a coffee shop, same as me except he's crushed under piles of student loan debt. So you can be as high and mighty as you like but the truth is staring you right in the face. People with degrees and those without are in the same boat going in the same direction except one is more broke from loans and debts than the other. Maybe in five or ten years that'll change if the economy recovers. But when you're paying through the nose for your college education by working a minimum wage job that barely pays your living expenses, let alone college expenses, you don't have five or ten years to wait for things to get better.
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Simon Baybrooke-Gibbens is a self-confessed
The Web designer – "Si" to his friends – is British, but just got married in Alabama. But first he and his now-bride, Claire, stopped off for a bit of shopping at the World of
Si has 100 items in his
'The most expensive thing I've brought to sell'
My bottle by American designer Britto. He did a series of six Coke bottles. It was actually a wedding present from my wife. In return I gave her a necklace made from the rim of a Coke bottle. I love the Britto bottle, but I know I there are more out there.
We got married in Alabama, and Coke was a big part of our wedding. In fact, our to-do list was: 1. Visit the World of
'My favorite piece'
Is the National Champions Crimson Tide Coke bottle. I've got another one of these – it's at home with our wedding flowers in it. One of my cousins works for Alabama University and that's their football team. They were national champions again this year so Coke did a set of bottles just for Alabama. I also like the gold


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Psychometrics is a field of study within psychology focusing on the theory and techniques of assessment. Psychometrists study the differences between individuals by examining personality characteristics, abilities and attitudes. The first instruments used for psychological assessment involved testing an individual's intelligence, such as the Stanford-Binet IQ test. Psychometrics has evolved to include testing for all aspects of human personality. With the appropriate education and experience, you can embark on a career in the psychological speciality of psychometrics.
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- Challenging
Other People Are Reading
- 1
Obtain a bachelor's degree in any branch of psychology. More education is required to become a psychometrician, but you must have post-high school education in the field of psychology from an accredited institution of higher learning.
- 2
Obtain at least a master's degree. Psychometrists must hold a minimum of a master's degree and most even have a doctorate degree. It's common for most psychometricians to have a master's degree in psychology.
- 3
Participate in a graduate internship. Study psychometrics with a licensed professional while you are continuing your education. This provides you with valuable knowledge and experience in the field of psychometry.
- 4
Obtain certification from the Board of Certified Psychometrists (BCP). This national certification may assist you in obtaining a job in some states. BCP credentials are considered to be the gold standard in the field of psychometry and will increase your value and credibility as a psychometrist. To take the exam to become board certified, you must have a bachelor's degree from a regionally accredited college or university and a minimum of 3,000 hours of testing experience, or a master's or doctorate degree from a regionally accredited college or university and at least 2,000 hours of testing experience. The exam is given once each year. The date and location can be obtained from the Board of Certified Psychometrists.
Tips and warnings
- Take a variety of math and statistics courses. You will find that many psychometrists have extensive coursework in statistics due to the nature of psychological assessment. Psychological testing requires knowledge of statistics in developing, reading and interpreting test results.
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100 comments found.
Hi Sike. Thanks for your awesome plugin. Unfortunately, I didn't know what Visual Composer was, and I don't have it – so I can't use this plugin. Are you able to refund my money please? I have literally only just purchased it and won't be able to use it. Thank you, Kiara Purchase code: d1175e51-9998-4d25-ba28-2926e5ac1a98 PayPal details: email@example.com
Sorry right now the refund doesn't cover this.
Hello Sike, 1- How do we change the font for the image overlay content (or for the flipbox)? 2- Both image overlay and the flipbox seem to have problems working with the frontend edit of Visual Composer. I make changes and it doesn't show any of them on the frontend edit page. Any way to solve this problem?
Hi, right now it doesn't support change font family in the editor, please try to customize via custom CSS. And the frontend editor of Visual Composer is very limit now, please try to edit the content in the backend of WordPress.
We are not seeing the flip boxes actually flip in IE11. All other browsers and mobile are great. This is the only one we are having trouble with. Can you help us? Thanks so much!
Hi due to the IE CSS3 limit, right it doesn't support the flip transition.
Do you have a solution for IE11 so that we can have the flip boxes for all other browsers, but the text on the back of the flip box will show below the text from the front of the flip box in IE11? Thanks again!
Hi, great plugin, but customization for the font-family is hard. I tried to change the font-family in custom css, h4 .cq-flipbox-title {font-family:roboto;} but that didn't work. I also tried to be more precise, including the other classes, but I would appreciate some help on how to achieve this. thank you in advance, Fran
h4.cq-flipbox-title { font-family:robots!important; }Regards
Using the parallax item, the image gets deformed to about 1500 pixels, whereas it's only 1000 pixels and I would like to keep it this size.
Adding custom CSS didn't help, as the element is styled inline.
Changing the cover ratio didn't help, quite the contrary: it moved the image off-frame.
Can you help? Thanks!
Hi I have installed the plugin, Flip Box – https://codecanyon.net/item/visual-composer-addon-image-overlay-flip-box/10366019 but I can not find the settings for WordPress How do I add photos to flip stand, how to use it Thanks
Hi, please make sure the Visual Composer is installed in your site. Then you can access it in the backend menu of VC. If you can find it on the menu, try to navigate to the setting of VC, enable the elements under the Role Manager.
On the product page is not written must buy Visual Composer If I knew I would not buy from you, It's not fair
any resolution to this? i'm having the same issue. looks great, but when you go back to the page, the photos, etc. do not render.http://oxfordandbond.com/marathon_advisors/about_marathon-advisors/
İt's fixed height guys your not can use it in mobile…
hi can you help me? is there a way of stopping the flip box from flipping? just need the front showing not the flip
Hello the add appears transparent on Safari while works well for Chrome, Mozilla. Kindly assist to fix the issues As soon as possible.
Does it work with all the browsers?
Is there a quick way to add a 3d effect to the flip box as in here: http://themenectar.com/demo/salient-service/ when you hover over the boxes text and images appear to be lifted off the box
Just purchased the plugin and looks good, though am having some issues straight away.
I am using the image overlay and certain fields are not taking effect such as:
1. Size of overlay – changed to 300×300 but remains the default 2. Overlay content color not changing from default 3. there is a background colour I have to use css to remove e.g. .cq-imageoverlay { background:none !important; } 4. No color for title/content hover.
Hi thanks your feedback, 1) Please try 300 only, which stand for 300×300. 2) Have fixed it, will be available in the next update. 3) You can try set the alpha of background color to 0 in the backend. 4) Right now it doesn't support hover color, maybe add it in the future update.
Can you confirm if this plugin has a installable WordPress package?
Hi, please unzip file download from CodeCanyon first, the installable plugin package is inside it.
Yes but this causes issues when installing through Envato Market plugin. See here https://github.com/envato/wp-envato-market/issues/54
Is it possible to create 2 variation e.g. 1 for install only and other for all (includes docs etc).
Hi, i need to get some basic list item formatting or a class onto my back card copy. How can i turn on html formatting or make a code change so that it supports this?
Hi, you may have to customize the php file to add more advanced html content.
Hi! Can this be used inside the grid builder? I would love to add a button to my grid that flips over the item to show an image. Can it be done with this plugin?
Hi you can see the effect in the demo. And yes, you can add it inside a column in a row via Visual Composer.
I've just updated Visual Composer and the Back Card Content has disappeared. I also can't get it to save when I type it back in. Are you working on an update for this? Thanks!
Hi, please send me your page and WP account via the contact form in my profile page, then I can try to help.
Does the add-on do the following: I want to have 5 images in a row and when the user Hovers an Image, to show a List of "Linkable Text Options". Like this demo image: https://s24.postimg.org/qru33d945/qwe.png
Thank you for your help
See the reply in another item.
Why is this not responsive? When I set a height and width – it's not responsive. And when I set both to 100% the images disappear. Please help!
Hi, which element you're referring to? You can send me your preview page too.
Some plugins not working with my theme
I haven't had a chance to go through them all yet but I am having issues with the following:
Type Writer (blank page) Draggable Timeline (plugin elements visible but don't work) Zoom or Magnify (blank page)
I am looking to get the Draggable Timeline working. When added it results in a blank page. Are you abel to assist with this?
Wordpress: Version 4.7.2 PHP: Version 7.0.0 Visual Composer Add-on – Image Overlay & Flip Box: Version 1.4.1
Hi, but the elements you're referring to is not included in this plugin. Something wrong here?
Sorry posted in the wrong support page. I was referring to Visual Composer Extensions Addon All in One
I have reposted in that comment section .


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The Christmas and New Year's period are a fun and festive time of year. So when January rolls around, many of us want minimal fuss when disposing of our Christmas Trees, wrapping, packaging, etc.
Use our handy recycling and disposal guide to assist you with this holiday season's waste:
Artificial Christmas trees cannot be recycled, but there are many ways we can reuse them if they are still in good shape:
Real Christmas trees can be picked-up in the special kerb-side pick-up, click here to find out more.
ALL decorations, lights, tinsel, nails, stands, tree bags MUST be removed.
These popular Christmas tree decorations can be disposed of in the General waste (red-lid).
If they are still in good condition they can be taken to the Salvage and Save Shop at the hard waste- Resource Recovery Centre, or the e-waste can be dropped off FREE at the transfer station.
Christmas cards that are 100% paper can go in the recycling bin (yellow-lid), staples do not need to be removed.
If you have received Christmas cards that are not hazardous or recyclable place in the general waste bin (red-lid).
If you are feeling creative you can always turn your cards into new gift tags, cards, or wreaths for next year. Find out more here.
Christmas crackers make a fun addition to Christmas Lunch/Dinner. You can place them in the recycling bin (yellow-lid bin).
Before doing so make sure you remove any decorations including ribbons and toys.
Drink bottles and cans can go in the recycling bin (yellow-lid), remember some cans and bottles have a 10 cent refundable deposit which you can drop off at the Elizabeth or Munno Para Scout Recycling Centre.
Any cardboard cask boxes can go into the recycling bin (yellow-lid) and place the cask bladder in the general waste (red-lid).
*Do not crush cans or bottles.
Festive food leftovers can go along way with many recipes on the internet to help with tasty turkey and ham leftovers.
However, for scraps you can put them in the organics bin (green-lid) this includes:
The organics bin is collected every two weeks, but if the bin gets smelly just throw some grass clippings or leaves to neutralise the smell.
These items can be scraped clean and put into the recycling bin.
Styrofoam cannot be recycled. Rinse clean if necessary and place in the general waste bin (red- lid bin).
If the trays/foils are mostly clean and rinsed, they are accepted for recycling (yellow-lid), dispose of lids that are not 100% aluminium and heavily soiled wraps/trays in the general waste (red-lid).
*Foil needs to be in balls of at least 3 inch diameter.
If the ornaments are in good condition consider donating them to an organisation that can use them.
Ornaments that are Glass, clay, treated-wood and plastic place in the general waste bin (red-lid).
Ornaments that are 100% paper can be recycled, If they have been decorated with noodles, photographs, ribbons, and other decorations remove and place those items in the general waste (red-lid).
Plastic casings go into the general waste bin (red-lid).
Streamers and cardboard disc goes into the organics bin (green-lid).
Tinsel is a beautiful way spruce up your Christmas tree however it is not great for the environment. We recommend reusing your tinsel as long as you can before placing it in the general waste bin (red-lid).
Also remember! The internet is a great source for crafting the old into new items. Why not get the kids involved for a school holiday project?
Toys and gift wrap/ packaging can be tricky to dispose since not all parts are recyclable.
Find out below what items go in what bin below:
Toys and gifts come in a combination of cardboard and hard plastic. Separate the cardboard and hard plastic before placing both in therecycling bin (yellow-lid).
*Remember to flatten boxes to make room in your recycling bin.
Gift bags are great as they are easy to store away and reuse again. If it is damaged the bag can be recycled (yellow-lid) but any ribbons, handles or decorations will need to be removed and placed in the general waste (red-lid).
Ribbons, ties, cords, and raffia are great to be reused for future presents and crafts.
You can look up some great craft ideas here.
If they cannot be reused, please place them in thegeneral waste (red-lid).
Paper wrapping can either be stored away for reuse or placed in the recycling bin (yellow-lid).
A great idea for the creatives! Mix it up a bit by using newspaper, tea towels or bags for gift wrapping. Re-useable ribbon also makes a great alternative for sticky tape. You can also give your presents a personal touch by using wrapping made from Children's artwork. Find out more about these tips here.
Tissue paper can go into the compost or organics bin (green-lid), make sure to remove any sticky tape and ribbon.
Foil or plastic wrapping, this includes cellophane can be placed in the general waste bin (red-lid).
Unfortunately wreaths and table decorations consist of too many materials, so they are best placed in the general waste (red-lid).
Non-alkaline batteries are banned from landfill and CANNOT be put into any of the kerbside collection bins.
However, batteries can be dropped off at a Playford Library or ALDI shop.
Great saver idea! Purchase rechargeable batteries and a charger, as they have 32 times LESS impact on the environment than the disposable batteries, after a few uses you can save money as well as energy.
Download the My Waste app on GooglePlay for Android or the AppStore for iPhone to keep up to date with your NAWMA news and information.
Info updated from OnkaparingaCity.com


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Help others choose the best products...Write a review
|Price incl. VAT
|£57.43 £45.19
|Price excl. VAT
|Return products within two months £2.26 Possibility to return products within two months
This Italian cutie for you and your loved ones a few moments to prepare delicious coffee with a captivating aroma. Moka pots are now returning to our homes, because under favorable purchase price offered excellent coffee prepared in the traditional way, without unnecessary fuss and complexity. This device is also electric so it will not occupy the plate on the stove. Creative design adheres to centuries of proven octagonal shape, thus aluminum body mugs excellent heat conductor.
Specifications can be changed without notice. Images are for illustrative purposes only. Retail Price (MSRP) means the manufacturer's/supplier's suggested retail price.
Link to Manufacturer's Website: Enter link to the external review


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Bonehead is a short film about a tragic fire / murder in Georgia circa 1985 and the effects it had on a young gay boy who was friends with one of the victims.
A tragic fire in a small Southern mobile home park disrupts lives including an adolescent boy who was good friends with one of the victims of the fire.
When investigations reveal a horrible crime the child most deal with some challenging truths about the violent nature of others and himself.
Burdened by his guilt and conflicting ideals of his community the child's future seems doomed to be darkened by the tragedy forever.
Written, Produced and Directed by Matt Lamb
Wes Miller – Bonehead
Matt Lamb – Preacher
Roman Lopez – Stunt Double
Steven Santillan
Andrea Garza
Written, Produced and Directed by Matt Lamb
Wes Miller – Bonehead
Matt Lamb – Preacher
Steven Santillan
Illustrations by:
Andrea Garza
Stunt Double:
Roman Lopez
Special thank you for contributions by:
Ann and Ralph Morris
Janie and Bucky Newton
Ashley Gibbs, Brett Gibbs and Family
Jessica Roberts Santacruz
Beth Lamb, Gary and Patsy Lamb
Matt Muma and Wild West Traders, Austell GA
BoneHead was made on location in Powder Springs and Talbotton, GA
In memory of Patrick Lamb
Copyright 2012 RuffHouse Studios. All Rights Reserved.


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| Next
Premium Camo Shotgun Case 48" Floating PVC Shotgun Case
Leapers UTG
Typically ships the same business day when ordered before 4pm CST.
The perfect case for storing, transporting and protecting your long guns! Keeps moisture out with its superior PVC outer shell and extra-thick padding. This case is loaded with features that would cost much more elsewhere: Super-durable, water-resistant 1680-denier PVC outer shell 48" long 2" of thick, high-density foam padding Soft linings to protect your gun Floats with gun in the case Fully reinforced stitching and edges for extra durability D-ring ends 10"x8" accessory pocket with hook-and-loop closure Sturdy wraparound carrying handle 1.5"-wide adjustable, padded, shoulder strap Tough-edge nose and back support Lockable high-quality, rugged zipper resists snagging Covered zipper blocks out moisture 4-color woodland camo pattern Imported Air freight charges will be $50.00 per case
Be the first to review this product.


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Without knowing it, we are our own worst enemies - all that rubbing and scrubbing to remove your eye make-up pulls your delicate eye contour downwards, creating bags and lines that age your skin! I cr...
published 18/03/2012
AdvantagesRemoves all makeup quickly
DisadvantagesQuite oily in consistency and a little overpriced
published 13/12/2010
AdvantagesRemoves heavy, persistent makeup in one wipe and thoroughly removes lip makeup
DisadvantagesSlightly too pricey and it feels quite greasy on the eye lids.
published 28/12/2011
AdvantagesDoes its job very well
Disadvantagesslightly greasy residue, expensive


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Greetings Guest!!Dismiss Notice
In order to combat SPAM on the forums, all users are required to have a minimum of 2 posts before they can submit links in any post or thread.
Do you need a few Spring Cleaning points?
Discussion in 'UO Lake Superior' started by Nafaustu, Sep 13, 2008.


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I agree with Steve about boredom, and especially about crating. My dogs (3
right now) are always raised in crates and they consider it HOME, sort of a
safety blanket. When they get tired they go crawl in the box and go to
sleep. It's not a given how a mature dog will react to a new idea. If you
decide to start with a box, leave it open for some time (days) and put his
toys in it and toss a biscuit in there for him to fetch, now and then.
Don't make the crate punishment... make it fun. This is all IMHO, but it
p.s. Pardon the gender specific language, I have 2 boys 1girl.
This ends my Dr. Doolittle routine, but I have dog pictures if any body
wants to see !! shan


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Holiday houses and apartments in North Bruny
» Huon Valley-D'entrecasteaux, TAS, Australia
3 properties found.
Bruny Island Accommodation Services
Holiday house - Huon Valley-D'entrecasteaux - in South Bruny
from A$155.16
Bruny Ocean Cottage
Holiday house - Huon Valley-D'entrecasteaux - 12km from North Bruny (Adventure Bay)
from A$190.00
from A$160.00
Displaying 1-3 of 3 results.
Click on each property location for details


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TCM Archive Materials VIEW ALL ARCHIVES (0)
Recent DVDs
Mrs. Miniver ... Memorable spirit-lifter about an idealized England that tends it's prize-winning... more info $15.96was $19.98 Buy Now
Forever Amber ... Linda Darnell, Cornel Wilde, Richard Greene, Jessica Tandy. A penniless young... more info $18.36was $19.98 Buy Now
Sneakers ... Robert Redford, Sidney Poitier, Dan Aykroyd, River Phoenix. A high-tech security... more info $5.95was $5.98 Buy Now
The Non-Stop... Originally released in 1926. Directed by Emilie Johnson, Emory Johnson. Starring... more info $10.95was $16.95 Buy Now
That Forsyte... Errol Flynn, Greer Garson. Beautiful, breathtaking Irene Forsyte: men adore... more info $15.96was $19.99 Buy Now
TCM Greatest... Features Lassie Come Home, Son Of Lassie, Courage Of Lassie and Hills Of Home. more info $11.95was $19.98 Buy Now
|Also Known As:
|Cause of Death:
|Birth Place:
|Cast ...
Cast (feature film)
During the Great Depression, a common-man hero, James J. Braddock--a.k.a. the "Cinderella Man"--was to become one of the most surprising sports legends in history. By the early 1930s, the impoverished ex-prizefighter was seemingly as broken-down, beaten-up and out-of-luck as much of the rest of the American populace who had hit rock bottom. His career appeared to be finished, he was unable to pay the bills, the only thing that mattered to him--his family--was in danger, and he was even forced to go on Public Relief. But deep inside, Jim Braddock never relinquished his determination. Driven by love, honor and an incredible dose of grit, he willed an impossible dream to come true. In a last-chance bid to help his family, Braddock returned to the ring. No one thought he had a shot. However Braddock, fueled by something beyond mere competition, kept winning. Suddenly, the ordinary working man became the mythic athlete. Carrying the hopes and dreams of the disenfranchised on his shoulders, Braddock rocketed through the ranks, until this underdog chose to do the unthinkable: take on the heavyweight champ of the world, the unstoppable Max Baer, renowned for having killed two men in the ring.
A biopic inspired by true events in the life of renowned boxing manager Jackie Kallen, a middle-aged Jewish mother from Michigan, who guided the careers of several, hi-profile, professional boxers, including four world champions such as: middleweight champ James "Lights Out" Toney, as well as Bronco
Telefilm depicting stories from the book "Rescuers: Portraits of Moral Courage in the Holocaust," involving the courageous efforts of non-Jews who risked their lives to rescue victims of the Holocaust. In "Malka Csizmadia," young Malka befriends and falls in love with Szarany, a man from the Jewish
Cast (TV Mini-Series)
Please support TCMDB by adding to this information.Click here to contribute


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commented on a Explorer of the Seas cruise review. - "why do you think you had a great experience and many people are complaining about the food and crowds?
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Paper Mache Animal Decor Christmas Ornaments Fox and Cat
Out of stock
To add this product to your wish list you must Sign In or Create an account
FREE SHIPPING available on minimum spend of $ 35 and above Click for more information
- Paper mache hanging ornaments for Christmas tree
- Fox; 3 inch
- Cat; 3.75 inch
- Handcrafted and hand-painted
- Made in Kashmir India
DESCRIPTIONCelebrate this festive season with this unique set of animal pendants for your Christmas tree. This is a set of mountain fox and a cat. The fox is green in color that is decorated with Indian paisley designs over the green base and it stands on all four feet. The sitting cat is black and white in color, which is intricately painted over the base in order to make the cat seem more real with its face, legs etc. clearly visible. Both pendants have glossy paints over them that make them more appealing in their appearance. Although these are hanging pendants, they are suitable for home decor as well, as they both can stand perfectly over a flat surface without hanging them. They are light in weight so that if hung on the Christmas tree, it will remain sturdy at all times.
Shipping Policy Shipping Policy
- We currently offer FREE DELIVERY on all orders above $35. With free shipping, your order will be delivered 5-8 business days after all of your items are available to ship
- The large majority of our orders are shipped within 1-2 working days of the order, and delivered in 3-5 days from shipping.
- Some of our fast selling products are kept in the local fulfillment center of Amazon. Orders containing those products are fulfilled by Amazon, usually delivered within 2 to 5 working days
- Delivery to Your Door - Receive your purchase without leaving the comfort of your own home.Your order will be shipped directly to your door, and if you wish, you can have it gift-wrapped
- Estimated Arrival Date - The Estimated Arrival Date for our merchandise is displayed on product pages, in your shopping bag, and throughout the checkout process.
- We provide the Estimated Arrival as a guide to when your shalinindia.com order should arrive.
- We uses the best carriers in the business to make sure your order gets to you on time., and you can track your order online after it is shipped We deliver your orders to your street address only. We do not deliver to post box addresses
- In the unlikely event we are unable to deliver any items in your order, be assured that you will not be charged for that item, or you shall be refunded the full amount charged for the item.
- We ship on all week days (Monday to Saturday), excluding public holidays
- If you believe that the product is not in good condition, or if the packaging is tampered with or damaged, before accepting delivery of the goods, please refuse to take delivery of the package, and call our Customer Care or at email@example.com, mentioning your order reference number. We shall make our best efforts to ensure that a replacement delivery is made to you at the earliest.
- Please note all items will be shipped with an invoice. In case, inadvertently, the same is not received
- If your order meets the qualifications for Guaranteed Accelerated Delivery, we will refund the shipping costs if we miss our delivery promise
Return Policy Return Policy
- 100% Payment Protection : Not satisfied with your purchase? Facing an issue with your item? Get 100% payment protection on your purchases
- Returns : ShalinIndia stands behind the quality of everything we sell and hope you're happy us. If you're unsatisfied for any reason, you can return your item(s)
- Returns time: Our policy lasts 30 days. If 30 days have gone by since your purchase, unfortunately we can't offer you a refund or exchange.
- Refunds : Once your return is received, we will send you an email to notify you that we have received your returned item.
- If you are approved, then your refund will be processed, and a credit will automatically be applied to your credit card or original method of payment, within a certain amount of days.
- Late or Missing Refunds: If you still have not received your refund yet, please contact us at email@example.com.
- Items that you return should not be used, washed, altered/tampered or soiled.
- Replacements will depend on the availability of the item
- In case the replacement item is out of stock, we will refund your amount.
- Item is physically damaged/defective
- Varies from the description
- Wrong item delivered
- Wrong colour
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Notably home to one of the largest straw markets in the Caribbean, Port Lucaya has the best of both words: luxury shopping paired with hand made crafts. Plus, the view of the marina adds a particularly soothing ambiance that you won't find at most shopping centers.
Set on a five-acre peninsula that overlooks a marina, the architecture of the Port Lucaya Marketplace is a hodgepodge of styles. As you walk into the main entrance, you will encounter a Japanese style pagoda painted every pastel color imaginable. Then spread out over the property is 12 island-style buildings that house boutiques, restaurants, and 80 specialty stores. If the duty-free shopping weren't enough to draw you to the shopping center, perhaps one of the largest straw markets in the Caribbean will. Where else will you be able to find unique, hand-made crafts made by local artisans all together in one place?
Located near the center of Port Lucaya, Port Lucaya Marketplace invites tourists who are exploring and browsing shops in this part of Freeport. Whether or not you're staying particularly close, you should feel free to stop by.
If you can't find what you are looking for here, you will be able to find a couple of more miscellaneous retailers nearby.
|Ocean Motion Watersports
|Watersports Equipment Shop
|0.3 mi.
|Seashore Shop Centre
|Shopping Center
|1.0 mi.
Outside of shopping, you'll find at least a couple of local landmarks you could visit while you are in Port Lucaya. If visiting a museum sounds fun, plan on lingering at both Museum Of Underwater Exploration and Grand Bahama Museum. Additionally, you'll find some other fun activities and restaurants nearby.
Port Lucaya Marketplace accepts major credit cards.
Location: Sea Horse Road, Port Lucaya, Grand Bahama Island
Phone: (242) 373-8446
Secondary Phone: (242) 373-8632
Website: http://portlucayamarketplace.com/lucaya/
Email: firstname.lastname@example.com
Help us improve! We welcome your corrections and suggestions.


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25% off mugs, totes, and spiral notebooks. Use STRETCHFOR25
Sunny day, nice place and…. freedom. Bieszczady 2007 – in Poland.
bieszczady clouds grass mountain poland landscape
great perspective,,, love this shot, well done
Such a beautiful place, I love it!
Gorgeous photo!!! :-) That would be a wonderful place to lay down in the tall grasses? And just lay their and watch the clouds dance across the sky
Beautiful shot!
great expansive shot
wow!this ones amazing.i love the deep blue sky and the clouds look like theyre reaching out almost.good stuff!
Stunning shot!
Loading more work by houk...
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Oxmoor House
The Lime Crema really makes the recipe! If you don't have any mango on hand, pineapple makes a good substitute. Add a side of Cilantro-Lime Rice to complete your meal.
Serves 4 (serving size: 2 tacos)
+ Add To Shopping List
Mango Salsa:
1 3/4 cups diced peeled mango (about 2 mangoes)
1/4 cup diced red onion
2 tablespoons chopped fresh cilantro
Lime Crema:
1/2 cup reduced-fat mayonnaise
1/2 cup fresh cilantro leaves
3 tablespoons fresh lime juice
1/2 ripe peeled avocado
1 pound mahimahi
1/8 teaspoon salt
1/8 teaspoon freshly ground black pepper
Cooking spray
8 (6-inch) gluten-free corn tortillas
2 cups shredded red cabbage
Hands-On: 23 Minutes
Total: 33 Minutes
1. To prepare salsa, combine first 3 ingredients in a bowl. Cover and refrigerate until ready to serve.
2. To prepare Lime Crema, place mayonnaise and next 3 ingredients (through avocado) in a food processor; process until smooth. Cover and refrigerate up to 2 days.
3. To prepare tacos, preheat grill to medium-high heat. Sprinkle fish with salt and pepper. Place fish on grill rack coated with cooking spray; cook 5 minutes on each side or until fish flakes easily when tested with a fork. Remove from grill; break into chunks with a fork.
4. While fish cooks, warm tortillas according to package directions.
5. Spoon 3 ounces fish, 1/4 cup salsa, 1 1/2 tablespoons crema, and 1/4 cup cabbage onto each tortilla; fold in half.
Created date
August 2012
Nutritional Information
Calories 347
Caloriesfromfat 0.0 %
Fat 9.3 g
Satfat 1.5 g
Monofat 0.1 g
Polyfat 0.7 g
Protein 24.3 g
Carbohydrate 45.4 g
Fiber 4.6 g
Cholesterol 83 mg
Iron 1.8 mg
Sodium 454 mg
Calcium 57 mg


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LaLa Vazquez
Casual rock
Alani Vazquez, also known as Lala, is an actress and currently also a personality of the MTV USA
Overseas she is very popular also because of her friendships within the star system: at her wedding she hosted a number of celebrities such as Justin Timberlake and Spike Lee.
This time we catch her while she is attending an event in Los Angeles. Here she went for a casual rock look: leathered skirt dotted with studs, perfecto, striped black and white top, a perfecto and black peep-toe ankle boots.
This is totally a glam-rock outfit that perfectly suits night-time events that are not too formal. This style can be interpreted as masculine, sensual or street, without giving up its intrinsic energy. Speaking of which, today's gallery give some us some hints about it.
In absence of studded pieces of clothing, try with metal jewellery, fringes, tears, leather shoes, smoky make-up, and a Ramones album playing on the background.
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A chat with Kristin Prim, the founder of The Provocateur, the site that publishes letters written expressely by leading women addressing the whole female world. Vogue.it presents you an exclusive preview of the letter written by Dresden Dolls' Amanda Palmer
Fashion shows are changing: Burberry and all that's new on the runways
Menswear and womenswear collections will walk together and will be on sale right after the show. These are the news from Burberry but the evolution is generalized: runway shows are changing