text:
Here is a hypothetical possibility that we cannot rule out. It is predicated on two major - but very possible - 'ifs.'
If late in August Obama is ahead in the national polls by double digits - say 52-40 - and equally ahead in the Electoral College by say 290 to 170;
And if Obama's fund raising is just cruising along - as expected - to where he will ultimately raise $300 million to $400 million vs. McCain's $85 million from the Treasury;
If we have that scenario - McCain trailing badly and certain to be outspent by 3-1 or 4-1 - then McCain is going to have to gamble and throw a Hail Mary pass to get himself back into the race.
Already the above scenario is taking shape:
Obama opted out of the federal election system because he knows he can raise that $300 million - or more - from his 1.5 million regular donors.
Obama is setting up shop in all 50 states with paid staffers - an unheard of development for Democrats. He is really going for a Big Win in a Big Year for the Democrats.
Obama is already airing a very effective new bio ad in 22 states - including several like Montana, North Dakota and Georgia which normally are ceded to the GOP. But clearly Obama has the money and the will to stress the McCain operation by expanding the states he will make a serious attempt to win.
MoveOn.org's very effective 'Baby Alex' ad is also rocking the McCain campaign. While airing nationally on cable and in just a few states locally, this type of independent expenditure used to be the specialty of conservative groups. But not this year! Apparently there is no anti-Obama money lining up to take on the Democrats this year. McCain - who castigated the 527s in the past - will have none helping him this year. Sweet justice, eh?
So, with all of the above in force this year, McCain by the end of August may do the following:
Once the Olympics are over and Obama has made his Veep selection (the Democratic Convention is the last week of August and the GOP's is Sept. 1-4), McCain will be a desperate candidate. His last chance to become President will be slipping away from him. He will be facing a fall campaign in which he is swamped by a better-funded candidate who is playing in states that McCain is now having trouble defending. He will see the possibility of getting blown out and of being a forgotten loser-for-all-time like Bob Dole, Mike Dukakis and George McGovern.
So he will need to do the unexpected:
He will seriously contemplate selecting New York City Mayor Mike Bloomberg as his running mate.
Because once he is on the ticket Bloomberg can then write out a check for $500 million for the campaign and - BOOM - the McCain/Bloomberg ticket suddenly can outspend Obama by two-to-one!
How is that for a shocker?
Would it work?
Probably not.
Bloomberg left the GOP in a huff two years ago and the thought of the Republican Party nominating an apostate for Veep is indeed hard to believe. But it is also hard to believe that McCain is the GOP nominee. Nothing should surprise us this year. Bloomberg is even more liberal than Obama! He is pro-choice, pro-high tax and way 'out there' on many issues. But he is rich and will spend the money - and McCain and he are friends, too.
Will Mccain do this?
He just might if he is trailing badly in the polls and in fund raising.
And, if he did it, he'd have the money to compete. But it would seem so transparent and phony as to cause McCain to lose by an even larger margin.
Still, don't rule this out. It is a wacky year and McCain is a total wack-job.
There is no telling what he might do.
The new spate of Quinnipiac polls from Florida, Ohio and Pennsylvania have rocked the GOP political establishment.
Here is the brief overview from Quinnipiac:
This is the first time Sen. Obama has led in all three states. No one has been elected President since 1960 without taking two of these three largest swing states in the Electoral College. Results from the independent Quinnipiac (KWIN-uh-pe-ack) University polls show:
Florida: Obama edges McCain 47 - 43 percent;
Ohio: Obama tops McCain 48 - 42 percent;
Pennsylvania: Obama leads McCain 52 - 40 percent.
In the three states, Obama leads McCain 10 to 23 percentage points among women, while men are too close to call. The Democrat trails among white voters in Florida and Ohio, but gets more than 90 percent of black voters in each state. He also has double-digit leads among young voters in each state. "Finally getting Sen. Hillary Clinton out of the race has been a big boost for Sen. Barack Obama. He now leads in all three of the major swing states, although his margins in Florida and Ohio are small," said Peter A. Brown, assistant director of the Quinnipiac University Polling Institute.
Believe me: these results - especially in Florida (thought to be secure for McCain) and Ohio (very winnable for the GOP - especially with Governor Ted Strickland stating he would not accept the Veep slot from Obama) - have shaken the McCain campaign and renewed the fear that an anti-GOP, anti-Bush tsunami is rolling in this fall - and it may take not only McCain along with it but dozens of GOP House and Senate hopefuls, as well.
Now, some questions need to be raised:
What can McCain do to reverse this pro-Obama trend?
Is it more anti-GOP - or anti-McCain? Or, to put it another way, could a different GOP candidate do better than McCain?
McCain seems mired at 42% - in these state-by-state polls and in the recent ABC and Gallup national polls. Why can't he grow above that?
There are still four and 1/2 months to go before Election Day. What events can shake up this race?
Some attempts to answer/analyze:
John McCain is - technically in the TV era - a disastrous, awful, unattractive, inarticulate, boring, mis-guided and not-too-bright candidate. Period. Plus, he doesn't want to run the kind of campaign necessary to destroy and thus to defeat Obama. (Witness Mccain's prohibition of even mentioning Obama's middle name - Hussein.)
McCain as a former POW is a valuable political biography. But his conflicted personality and 'inside-the-beltway' contradictions (he is surrounded by lobbyists after decrying government pork) undermine what could have been a pristine personal story: Mr. POW Hero Comes to DC and Takes On the Corrupt Establishment.
Instead, the story has been turned into: Mr. Principle Becomes A Bush Lackey and A Corporate Suck-Up - Who Switches Positions More Often Than John Kerry.
McCain's overall campaign strategist - Charlie Black - is a smart guy who will maximize all that is possible with such a weak candidate. But he does not have much to work with.
There is a built-in resistence to Obama that will still help McCain - or another GOP nominee should McCain not be nominated in September (still a possibility). Obama is at the mid-to-high 40s; a moderate, non-leftist Democrat would today be in the mid-50s and be virtually unbeatable in November.
The GOP must show the American voter that Obama is too risky - and too radical to be entrusted with the Oval Office.
The problem is that McCain is tied to Bush's hip, is bad on TV and doesn't connect with voters.
And McCain's base - the so-called Mainstream Media - is already abandoning him for their real love: Obama.
Do you know what is needed? A real conservative GOP candidate to emerge and to challenge not only McCain but also the fact that the media chose our nominee for us.
The American people distrust the media.
It is too bad both nominees are creations of that media.
On Tuesday the Obama campaign suffered the biggest blow to their chances in November since the emergence of the Reverend Wright videotapes.
The stunning - and totally unexpected announcement - on NPR by Ohio Governor Ted Strickland that, under no circumstances, would he run/accept the Veep slot with Obama threatens Obama's chances to garner the 270 required Electoral Votes.
On the popular show, "All Things Considered," Strickland was
asked if he was angling to become Senator Barack Obama's vice-presidential running mate.http://www.npr.org/templates/story/story.php?storyId=91360615 He said, "Absolutely not. If drafted I will not run, if nominated I will not accept, and if elected I will not serve."
This unexpected announcement removes the most likely Veep choice for Obama.
Strickland was crucial to Obama's attempt to win two of the three key swing, battleground states: Florida, Ohio and Pennsylvania.
McCain is way ahead in Florida and most astute political pros do not think Obama can make the Sunshine State even close in the fall.
Pennsylvania, which in recent years has gone into the Democratic column, is slightly leaning toward the Democrats again this year.
So it is Ohio - like 2004 - which may hold the key to winning in November. And for that reason yours truly has been telling everyone that Obama's most likely Veep choice was Governor Strickland. A former US Congressman and an ordained minister, the Governor would have been an ideal choice - and the type the Democrats have not recently made.
Joe Lieberman in 2000 and John Edwards in 2004 were total failures because they brought nothing to the ticket: no state, no region and no constituency.
The Veep pick must give the Presidential nominee something he would not have had without it.
In Strickland, Obama would have had a more than even shot to win the Buckeye State, plus the Governor was for Hillary so he would help heal that divide. And his executive experience would help, too.
If the revelation of Reverend Wright on those explosive videotapes was the single biggest blow to Obama so far, this sudden announcement by Ted Strickland - while not as sexy or seemingly news-worthy - is indeed a major setback for Obama's ability to garner those 270 Electors.
He now may have to revamp his strategy and focus on other states which are more difficult for him to win, such as North Carolina, Colorado and Virginia. Democrats do not win these states in Presidential elections; maybe this year will be different. But the Obama brain trust knows their odds are worse in these states.
So, who now goes to the top of Obama's Veep list?
Is it Bill Richardson (supposedly has a zipper problem, and can he really deliver the Hispanic vote)? Is it some retired general (dubious, would be a huge mistake, generals are not usually adept at the skills necessary to be a candidate, risky to bet the ranch on an untested candidate), Bill Nelson of Florida (a pretty boy lightweight who probably couldn't deliver Florida anyway so it would be a wasted pick) - or does this surprise by Strickland put Hillary back in the running?
Only time will tell.
On the GOP/McCain side, Mitt Romney is ahead in the Veep stakes - mainly to help in Michigan and to help raise money.
But there is a long, long way to go - even to the two conventions.
No decisions will be made until mid-August.
Hillary's campaign is almost toast - or so the media says.
But, for the sake of argument, let us assume that sometime in the next few days or weeks Hillary concedes and then suspends her campaign. And then she announces her "unqualified" support for Obama. And then she publicly campaigns for Obama.
Here is what will be said:
"Hillary is taking the high road. She is helping unite the Democratic Party in opposition to McCain and the GOP. She is also preserving her right to run again in 2012 if Obama should lose this year. She is trying to be seen as a 'team player' and a 'good loser.' In sum, she is taking the High Road.
But here is what the truth will be:
Yes, she will look as though she is taking the high road; in reality, she will also be taking the lowest road imaginable. Here is how:
While smiling at Obama and speaking sweet sounds of support, Hillary (and Bill) - bitter and vengeful - have a team of private detectives fanned out gathering salacious dirt on Obama. The rumored tape of Michelle Obama spewing anti-white diatribes is just such an example of the Clintons' dirty work. So is another rumor circulating in Democratic circles of Team Clinton having the dirt on Obama and a woman.
You can bet Team Clinton has a Dirty Road Plan: try to expose enough dirt on Obama - leaked through cut-outs - to derail him before the August 27th Democratic Convention. The Clintons figure if enough bad stuff comes out before then, the Super Delegates will see Obama as a certain loser in November and may still switch their votes to Hillary at the convention.
Or, if the dirt from the Low Road Plan comes in after the convention, Team Clinton will either leak it out or give it to McCain to use (if that wimpy campaign has the guts to attack Obama).
Her Low Road Goal? Have Obama lose in November - whereupon she will immediately begin her I Told You So campaign. She will remind every Democratic Party official and leader that she and Bill had told them all along that she could and would have won in the general election and that Obama would not win. This is the beginning of Hillary 2012.
This is what Team Clinton is planning.
Anyone who honestly accepts them on their word is a total fool. The Clintons are a political machine dedicated to one thing only: acquiring and then keeping political power. Period. Finis. Done. That's All, Folks.
So, ignore their High Road words - and instead focus on the obvious Low Road they are on. There are going to be new revelations about Obama. And he may still not be the nominee.
The Clintons are masters of the Low Road.
And it may still lead them to their Promised Land.
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text:
Last edited by Full House; 01-08-2014 at 22:21.
Cramping like period pains can sometimes be a sign of pre term labour. With your BH can you feel your tummy tighten if you put your hands on your belly? Does the cramping get worse with the BH? As a PP mentioned, take some paracetamol, pop on a heat pack and go to sleep. If the cramps don't go away/get worse/accompanied by any fluid loss, bleeding or decrease in your babies normal movements, call you hospital/midwife as you might need a once over. If they disappear completely then it's most likely normal pregnancy aches and pains. But if at any point you're not convinced it's normal, call your hospital/midwife. YOU need to know that everything is ok, and any doubt in YOUR mind is worth a check out. A mothers instinct is sometimes a lot stronger than we think.
Cramping for two days with suspected BH / contractions should be enough to take you off to your OB or even hospital. I had similar at 24 weeks last pregnancy, with the pain radiating down my in to my thighs and around my back, not relieved by heat packs, panadol etc. At first I felt silly by taking myself off to the hospital with what I thought I was going to be told was BH.
Turns out I was contracting due to a very large clot behind my placenta and it was serious. (Please don't let the story scare you, as it's not guaranteed by any means that it could be what is happening in your situation, but just an example of why you might want to get checked).
Rang the hospital they said the cramps might be from dehydration to rest tonight and drink plenty of water and if it hasn't stopped by morning to going
Thank you
If it helps, @BigMumma83, when bub was pressing on my cervix in a certain way, I definitely felt it in my clitoris and a couple of the girls in my DIG said the same thing. I think it's just the way you worded it.
As the others said, the cramping is very concerning but again, girls in my DIG had the same thing due to dehydration. Did you end up going to the hospital?
I had the same goblin
Because the clitoris continues inside by a couple if cm's, sometimes I would feel that sensation too
I also got bh 5 or so times a day from 28w. Started engaging at 34w
Spontaneous at 39w4d
Hi @BigMumma83,
How are you feeling today?
I'm 25 weeks and already I feel very heavy, sore and swollen 'down there'. My Ob/gyn said its just due to the pressure of baby and will most likely get worse. You may be feeling a numbness or tingling sensation. She checked to make sure it wasn't varicose veins.
Hope this helps.
With regards to the cramps, could it just be Braxton hicks? Im getting them already.
Last edited by LouLaa; 03-08-2014 at 13:20.
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Former USC quarterback Mitch Mustain was arrested late Tuesday night and held on a felony narcotics charge, Los Angeles Police Department spokesperson Bruce Borihanh confirmed Wednesday to ESPN Los Angeles.
Mustain was arrested at 8 p.m. PT on Tuesday. Bail was set at $30,000. Mustain has not paid bail and is still in jail, Borihanh said.
The LA Times reported "the arrest came as the result of a sting operation."
Mustain, 22, might be the nation's best-known backup quarterback. Considered one of the nation's top QB recruits in 2006, he signed with his home-state team, Arkansas, and he went 8-0 as the Razorbacks starter as a true freshman. But he opted to transfer to USC in 2007 after a highly publicized falling out with coach Houston Nutt.
At USC, however, he was unable to beat out Mark Sanchez and then Matt Barkley. While he only started one game at USC -- a 20-16 loss to Notre Dame this season, when Barkley was hurt -- he was still considered an NFL prospect.
This arrest, obviously, dims those prospects.
Mustain is the second former Trojan to be arrested this week. Former defensive end Everson Griffen, now with the Minnesota Vikings, was arrested Monday on suspicion of felony battery, when LAPD officers used a Taser to subdue him after a traffic stop near campus. Prosecutors told the LA Times "that felony charges would not be filed because officers were not injured and Griffen lacked a criminal history. The case is being forwarded to the Los Angeles city attorney's office."


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Edward Jones - Financial Advisor: Sean E Pigg, Securities, listed under "Securities" category, is located at 1590 Bonnie Lane Suite 104 Cordova TN, 38016 and can be reached by 9016244442 phone number. Edward Jones - Financial Advisor: Sean E Pigg has currently 0 reviews.This business profile is not yet claimed, and if you are the owner, claim your business profile for free. If you are not the owner you can invite this business to join by email or by phone.Browse all Securities in Cordova TN. Discover census data for Cordova, TN.
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40 CFR Part 233, Subpart G - Eligible Indian Tribes
58 FR 8183, Feb. 11, 1993, unless otherwise noted.
Title 40 published on 2015-08-22
The following are ALL rules, proposed rules, and notices (chronologically) published in the Federal Register relating to 40 CFR Part 233 after this date.
Title 40 published on 2015-08-22.
The following are only the Rules published in the Federal Register after the published date of Title 40.
For a complete list of all Rules, Proposed Rules, and Notices view the Rulemaking tab.


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full size
Product Details
Petite pencil skirt in lightweight wool
Petite pencil skirt in lightweight wool is rated out of 5 by 3.
Rated 5 out of 5 by macgirl from Perfect Skirt I ordered in black and went back and ordered a second in gray. Looks great for work with a jacket or blazer. I can't believe how great my J. Crew blazers look with the skirt. Everyone at works love my new look.
Date published: 2015-10-15
Rated 4 out of 5 by Katie from Bought this with matching jacket- great price! I bought this and the matching jacket in 0P. I think I got it 40% off, so that's a fantastic deal for a women's suit. The skirt is lined and I found that it fits TTS. I often have to get skirts taken in at the waist, but this one was perfect. It's also a nice color gray that can be used for all seasons--not a super light gray, but a darker, more substantial color. The only reason for my 4 stars is that the clasp at the back of the skirt was hidden so much it couldn't be accessed. The hook separated so a little metal piece stuck out. I had to take the skirt to the tailor to replace the hook and clasp. I appreciate trying to hide the clasp, but this one went a little too far! Not sure if this is an issue with all skirts or I just got a fluke. Overall, that was a minor adjustment to be made. Just something for the designers to keep in mind!
Date published: 2015-10-08
Rated 4 out of 5 by Claire from Lining seems cheap Looks great, but the lining seems like it's made of paper material, not smooth to the touch or against the skin.
Date published: 2015-06-25


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Kinsarvik Tourism & Travel Guide
Norway, the land of mid-night sun, is a paradise for nature lover....
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Places To Stay 55 Places
About Kinsarvik
Kinsarvik, named after the wild Kinso river which runs through the village, is the starting point for the hike to four stunning waterfalls in Husedalen valley. The first two are approximately half an hour simple hike from the starting point. The next two are a good 4 hours hike away, make sure that you have proper hiking gear before you venture on this trail. Coming back to Kinsavik, visit Hardanger Bestikk, the largest cutlery manufacturing unit in Scandinavia. The family run company organises a free guide tour everyday at 1pm. It give a sneak peak in the culture of the region.
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Ho Hum, Just Another Ambient Dub Masterpiece. From Japan This Time
Come on, people, we've been over this: ambient dub doesn't need any more masterpieces. We've already had Dubchek's Down Memory Gap Lane and the Orb's Adventures Beyond the Ultraworld and a few others, and they're all very lovely and psychedelic and expansive, and they're enough already. (Not to mention my whole drunken argument thing about dub being the ultimate expression of ambient music, and about how Eno and his ilk were already influenced by dub anyway. I'll save that for some other time.) The last thing we need is for some Japanese dude to come along and try to create some kind of beautiful thing full of echoes and synths and muted trumpet and lovely melodies.
But of course that's what Kazufumi Kodama A.K.A. "Echo" from Dub Station has done here. (I'll just call him Kodama from here on in, but that whole long moniker is what he's credited as, on the disc. So you know.) As a pioneer in Japanese dub—yeah, it's a scene, what of it?—with Dub Station, he's got the pedigree, he's put in the hours. So here, on this solo disc (originally released in Japan in 2000), he has constructed a loose concept album (don't worry, only two tracks have vocals) about space, and made it swing. Kodama has built a hell of a soundtrack for the sci-fi movie in his head, and it's a good thing that it's available here for the first time.
Kazufumi Kodama A.k.a. "echo" from Dub Station
(Red Ink)
US: 11 Mar 2003
UK: Available as import
He opens with the street-cred move of "Gate of Planet (Introduction)": you know a brother's serious when he busts out the kalimba. But this is just an opening trifle, and stuff gets serious on "Earth". This is a bass-heavy concerto for Kodama's jazz trumpet against his own synth-string arrangement. It's like a lot of ambient dub, with unobtrusively pretty melodies that don't try too hard and deceptively simple rhythm figures. If "Earth" sounds too facile on first listen, try it again; you'll notice the depth of the sonic layering and whisper "wow". Then you'll get how hip-hop the percussion line runs and say "dude", and then you'll dig the Miles Davisisms of the muted trumpet and be all like "yeahhhhhh" like a damned hippie raver punk rasta or something.
Let's just say here that Kodama is a really amazing trumpet player. He's not flashy, he doesn't try to impress anyone with the sheer volume of his notes-to-bar ratio, but he's great nonetheless because all he's trying to do is paint the corners with that trumpet. "Funky Planet" is the centerpiece of the LP, and it comes to life largely because Kodama plays that thang like he's muttering a love poem to the Queen of Sheba. Never loud, rarely blistering, always on point, Kodama nails moody Rastafari ("Stars") and smooth Caribbean lovers' rock ("Romance" with its steel drum sounds) while still sounding like himself.
Since Kodama is responsible for every single sound on this record except one vocal (UA's turn on "Moonlight Waltz"), he gets to get all playful. "Mars" finds him trading fours with himself in both piano-man and trumpet-jock modes; it sounds natural and cool, especially when the piano guy starts to do little free-jazz freakouts—because in comes trumpet-jock to save the day with soft cadences and honeyed tones. He'd be good if he was just a trumpeter but he isn't just an anything, and therein lies his power.
Actually, I think the aspect of this disc that I didn't get until the third or fourth listen is just what a great drum programmer he is. "Herb Yard" has a crazy forward drive based entirely on hi-hat and rimshot sounds, framing the horn flourishes the way they should be framed. "Romance" is all vibes and steel drums until you whip out the ol' headphones, at which point it comes alive with found footsteps and wind noises and fake vinyl crackles, and some really kickin' down tempo cymbal sounds. And "Waveless Wave (Dub Station Philharmonic Version)" is the slowest damned thing that you'll ever fall in love with, especially with all those faux-cellos and not-quite-oboes and I-can't-believe-it's-not-harp sounds echoing all around the mix.
Okay, so you'll hate this if you need speed and shifting textures and Loudness! in your music. (Uh, except for "45F", which brings in a humongous hip-hop beat and crashing cymbals, only to disappear after just 92 seconds.) And okay, if you're not down with the idea of a Japanese trumpeter making a truly wonderful ambient dub record, then . . . well, in that case, you're too far gone for me.
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Chick Quest 'Model View Controller' (album stream) (premiere) // Music
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Stone Foundation "Strange People" feat. William Bell (premiere) // Music
20 Questions: Nashville Singer-Songwriter Natalie Hemby
// Sound Affects
"Natalie Hemby's Puxico is a standout debut from a songwriter who has been behind the scenes for over a decade.READ the article


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Rock Dog
I can report that it enraptured and delighted, and most importantly, made quiet, the houseful of little kids and their nannies with which I watched…
I can report that it enraptured and delighted, and most importantly, made quiet, the houseful of little kids and their nannies with which I watched…
We need more directors willing to take risks with films like Get Out.
Roger Ebert on James Ivory's "Howards End".
"The Ballad of Narayama" is a Japanese film of great beauty and elegant artifice, telling a story of startling cruelty. What a space it opens…
An Ebert Fellow on films and programs from Sundance that focus on the environment.
A report on three documentaries from Sundance that tackle race relations in the era of Black Lives Matter.
A news brief on the Agnès Varda exhibition set to premiere at New York's Blum & Poe on Thursday, March 2nd.
The boy loves soccer.
Jana Monji journeys to the legendary Disney vaults to learn more about the creation of Disney's "Pinocchio."
Four honorees were celebrated during a special luncheon preceding the African American Film Critics Association awards on February 8.
The RogerEbert.com staff's Oscar pick for Best Picture.
The RogerEbert.com staff's Oscar pick for Best Director.
"Predator" begins like "Rambo" and ends like "Alien," and in today's Hollywood, that's creativity. Most movies are inspired by only one previous blockbuster.
The movie stars Arnold Schwarzenegger as the leader of a U.S. Army commando team that goes into the South American jungle on a political mission and ends up dueling with a killer from outer space. This is the kind of idea that is produced at the end of a 10-second brainstorming session, but if it's done well, who cares?
"Predator" is filmed very well. It's a slick, high-energy action picture that takes a lot of its strength from its steamy locations in Mexico. The heroes spend most of their time surrounded by an impenetrable jungle, a green wall of majestic vistas populated by all sorts of natural predators in addition to the alien. I've rarely seen a jungle look more beautiful, or more convincing; the location effect is on a par with "Fitzcarraldo" and "The Emerald Forest."
As the film opens, Schwarzenegger and his comrades venture into this jungle in search of South American officials who have been kidnapped by terrorists. They track and locate the fugitives, and move in for the kill. But as they find the bodies of team members skinned and hanging from trees, they begin to realize they're up against more than terrorists.
The predator of the movie's title is a visitor from space; that's established in the opening scene. What it is doing in the jungle is never explained. The creature lives in the trees, even though it seems to be a giant biped much too heavy to swing from vines. When Schwarzenegger finally grapples with it, we discover it is wearing a space suit, and that inside the suit is a disgusting creature with a mouth surrounded by little pincers to shove in the food.
Such details are important, of course. Stan Winston, who designed the creature, has created a beast that is sufficiently disgusting to justify Schwarzenegger's loathing for it. And the action moves so quickly that we overlook questions such as (1) Why would an alien species go to all the effort to send a creature to Earth, just so that it could swing from trees and skin American soldiers? Or, (2) Why would a creature so technologically advanced need to bother with hand-to-hand combat, when it could just zap Arnold with a ray gun?
At one point in the movie, the creature removes its helmet so it can battle Arnold mano-a-mano, and I was cynical enough to assume that its motivation was not macho pride, but the desire to display Winston's special effects.
None of these logical questions are very important to the movie. "Predator" moves at a breakneck pace, it has strong and simple characterizations, it has good location photography and terrific special effects, and it supplies what it claims to supply: an effective action movie.
Students of trivia might want to note that the actor inside the predator costume is Kevin Peter Hall, who also occupies the Bigfoot costume in "Harry and the Hendersons."
This guy must really be a good sport.
A review of Netflix's new series, Lemony Snicket's "A Series of Unfortunate Events," which premieres January 13.
This message came to me from a reader named Peter Svensland. He and a fr...
Our resident awards expert predicts who will go home with an Oscar on Sunday night.
At the ripe age of 89, Oscar can still be a notoriously picky fellow when it comes to what constitutes a contender fo...


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Houses for Rent in 65652, MO
|335 Listings Found
Oaklawn Estates is a premier residential community of single family homes in Rogersville, month, featuring high-quality homes, unique...
107 Bluegrass St, Rogersville, MO, 65742Details »
A recently remodeled and very nice 2 beds, two full bathrooms, garage for 1 car opener remote Duplex home featuring nice landscaping,...
212 Beatie, Rogersville, MO, 65742Details »
Cozy farmhouse on twenty fenced acres. Barn, paddocks, water spikets, out building and large pond. Hardwood flooring, gas fireplace,...
6409 E Highway 60, Rogersville, MO, 65742Details »
OZARK -\. TAN PLEX - NO PETS - CALL OFFICE FOR SHOWING INFO - 3 BEDs, two BATHs, 2 CAR GARAGE WITH OPENER, LIVING ROOM, KITCHEN...
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5776 E Gatehouse Drive, Strafford, MO, 65757Details »
Great home that has been remodeled located in the Turnberry subdivision and features 3 bedrooms, two bathrooms and a garage for 2...
5125 N 10th St, Ozark, MO, 65721Details »
This patio home is 1350ft of comfort with a nice master bedroom suite with a great bath and walk in closet. The living space has ten...
3520 E Lombard, Springfield, MO, 65809Details »
Beautiful custom built home in Ruskin Heights you don't want to miss! - This home is a MUST SEE located in the beautiful Ruskin...
3924 E Ridgeview St, Springfield, MO, 65809Details »
This is a great home located in East Springfield just past Hickory Hills golf course on East Cherry built in 2012. This home features...
829 S Pin Oak Drive, Springfield, MO, 65809Details »
This patio home is 1350ft of comfort with a nice master bedroom suite with a great bath and walk in closet. The living space has ten...
3520 G E Lombard, Springfield, MO, 65809Details »
This exceptional 3 beds two bathrooms home has many nice features including new floor coverings in living room, nice open 4N808R...
500 Wrenwood, Strafford, MO, 65757Details »
Duplex Triplex for rent in Springfield. Close to 4NMFZJ dining and shops, bright, gas stove, trash 4NMFZJ included.
3413 E Sunset St, Springfield, MO, 65804Details »
Walk to Fi ey River Park. Beautiful area. Range supplied. Large shop. Rent $875, Deposit $875 No Pets 4NM7F9 Applications are also...
402 W McCracken, Ozark, MO, 65721Details »
This brand new home is located in Hoovers Mill featuring 3 beds, two bathrooms and a garage for 2 cars. Quality construction home on a...
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Rental Terms Rent: $925 secure Deposit: $925. Close to 4NHPA0 dining and shops, bright, gas stove, trash included.
2005 E Jay St, Ozark, MO, 65721Details »
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True Interior's Story: Part II
Editor's Note: This is the second part in a series going behind the scenes at new ecommerce website True Interior. Co-founders Paul Rogers and Rob Carter are frank about the challenges and successes their ecommerce business has faced. Catch up on all the True Interior posts here.


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Tampa has a population of 303,447 and is located in Hillsborough county.
Industrial Design schools in Tampa charged students an average tuition of $13,050.00 if they were Florida residents, and $14,666.00 if they were non-residents, during the 2009 - 2010 school year.
The average cost of books and supplies for industrial design schools in Tampa is $1,144.00. Students who live on campus while attending a industrial design school in Tampa, can expect to pay an average of $8,078.00 for room and board. Students who live at home can expect average costs to be $4,926.00. There is one industrial design college in Tampa for students to choose from. 65 students were reported to have graduated from industrial design programs in Tampa in the 2008 - 2009 school year.
|Average Salary
|Number Employed in City
|Commercial and Industrial Designers


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George Clooney showed off his beard at the New York bash for the Film Critics Circle Awards at the glitzy Crimson club on Monday night.
Clooney was accompanied by his girlfriend, Elisabetta Canalis, an Italian television presenter and model, as he was given the Best Actor award for his performances in Up in the Air and Fantastic Mr Fox, ahead of the Academy Awards in March. Meryl Streep, who won Best Actress for Julia & Julia, chatted to Lynn Redgrave at the cocktail reception before dinner. She was joined by the former Sopranos actor James Gandolfini, who collected an award for In The Loop, in which he starred. The Armando Iannucci film, a spin-off from the BBC comedy The Thick of It, won Best Screenplay.
The Chairman of the New York Film Critics Circle, Armond White, made the most of meeting the director Kathryn Bigelow, who was named Best Director for her Iraq war movie, The Hurt Locker, which also won Best Film. The two had not seen each other since they were film students at Columbia University and White had always been a big fan of the music video Bigelow made for New Order in 1987, for the song "Touched by the Hand of God".
The Metropolitan director Whit Stillman was in deep conversation with the director of Summer Hours, Olivier Assayas, the winner of Best Foreign Language Film. The director of the British film Hunger, Steve McQueen, who won Best First Feature, stuck close to the film's star, Michael Fassbender.
In his acceptance speech, Clooney offered comforting words to the film critics who had voted for him. His dad had been a reporter, he said, and he understood how difficult it was for journalists losing their jobs due to the shrinkage of newspapers.Reuse content


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Online Promoter
Job Description
The candidate should have the following requirements:-
1. Basic computer knowledge required.
2. Social network marketing knowledge.
The candidate should fulfil the following job responsibilities:-
1. Promote online services.
2. Posting advertisements on internet.
About Company
United Capital Club was founded in 2012 with the single mission of delivering high quality Travel & Tourism services to the worldwide customers. United Capital Club is a state of the art futuristic company providing Travel & Tourism services by integrating people, processes and technology, to focus on the customers. UCC brings together the best in people, process and technology to ensure delivery of the highest quality services. The Club has been handling Inbound and Outbound voice and web based service center for our customers across India.
United Capital Club exists to understand the Travel needs of the customers & provide them the best-in-class services. We see ourselves as a strategic brand rather than a vendor. We engage with customers to establish and maintain long-term and high touch relationships.
Well before services are provided, we go to great lengths to seek to understand our customer's preference & priorities. We customize our business to fit yours. We are flexible, responsive and easy to do business with. As a brand, UCC is essentially a one stop shop for all your Travel needs. We understand the intricacies of our business and take the complete ownership to meet or exceed your expectations.


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TV star Yash Dasgupta will romance Mimi Chakraborty in the Birsa Dasgupta film. After a long 40 days of shoot in Turkey, Birsha Dasgupta's team wraps up their shoot on August 14. The unit including Mimi Chakraborty and the debutant star Yash comes back to Kolkata soon.
The film traces the journey of a gangster who meets a girl and falls in love.The Puja release made under the banner of Venkatsh Films, will have music by Arindom Chatterjee has been already scheduled for this Puja.
#Turkey Last day shoot #ThisPuja #SVF Production 101 @mimichakraborty @Yash_Dasgupta @BirsaDasgupta @iammony RT Fans pic.twitter.com/1yyES1zn8n
— Tolly Planet Bangla (@Tolly_Planet) August 15, 2016
Thanks to casting director @p00namjha.. Yash and Mimi di ke khub sundar Kore sajanor jonney.. Thanks a lott.. pic.twitter.com/vjc8HYaf6e
— Mim (@yashian_mim) August 14, 2016
P's Diary : YashD winning the hearts and minds in Turkey https://t.co/ziPKEgHPnO via @YashhD_FC
— Yash (@Yash_Dasgupta) July 26, 2016
Don't pursue happiness, create it 😊 pic.twitter.com/8Xt80xc9fk
— Yash (@Yash_Dasgupta) August 1, 2016


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Are you looking for Logistics Services in North Eastern VIC? Hotfrog showcases 40 popular businesses related to Logistics Services near North Eastern VIC! Find a specific suburb using the left navigation.
Wangaratta, Vic
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Armies of Dragons
By: Animoca Collective
Welcome to the walkthrough for the iOS game Armies of Dragons, if you are looking for ways to make it to the next level, and to improve gaming experience as a master player, you've come to the right place. Here you will find Armies of Dragons tips, tricks and guides, as well as the opportunity to share your own discoveries to help others move on and beat the game.
If you can't find the answer to your question you are welcome to post it in the comments section below. This way others who know the answer can help you by sharing their own tips and advices.
Armies of Dragons game app is developed by Animoca Collective and available on iTunes and Google Play. For more details on the app, check out our review.
- App name: Armies of Dragons
- Seller: Animoca Collective
- Category: Simulation
- Updated: Aug 26, 2014
Armies of Dragons iOS/Android Gameplay
Check out these tutorial for all levels of Armies of Dragons. We'll continue to update this list with the latest answers as new levels are released.
Armies of Dragons Answers
- Basic Game Guide Part One - game review ios / android gameplay
Hit like if this helped you!
Until the Walkthrough is completed, feel free to ask for help in the comments section.
Looking for a trick all your friends? Check out the Armies of Dragons cheats list to find collectibles guides, easter eggs and secrets.


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Sommer Cable HC Sinus Control 1,5m
Sommer Cable HC Sinus Control 1,5m, Phono-Twincable with ground line, Hicon HI-CM06 RCA-plug, golden, grounding cable with cable shoe on each side, length 1,5 Meter
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Deep...<quoted text>
That's only really true for people like right wingers and, I don't know, perhaps people with Down's Syndrome ...
The truth is what you want to hear so what you want to hear must be the truth.
A curious phenomenon to those who can think.
the legacy of who the network goes to is important to us (Current) and we are sensitive to networks not aligned with our point of view.
al-Jazeera was founded with the same goals we had for Current.
You may want to align yourself with Al Gorzerra, not I.


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Why Alex Rodriguez suspension might actually be good news for Red Sox
|01.14.14 at 8:01 am ET
Surprise, surprise.
The prevailing thought throughout baseball circles upon hearing about Alex Rodriguez's 162-game suspension was that the Yankees' newfound budgetary relief would be an American League game-changer. And it very well might be. Just not in the way many originally thought.
Yes, the Yankees will be getting about $24.5 million in wiggle room for the 2014 season thanks to the Rodriguez decision. But, depending on how Hank and Hal Steinbrenner view their business model, that actually may have made things more complicated for the New Yorkers while offering renewed hope for their AL East rivals.
Here's what you should know:
— Even with the newfound financial flexibility, the Yankees are right up against the $189 million luxury tax threshold. If Rodriguez's suspension was knocked down to the point where he would be playing a good chunk of the season, they most likely would have been over.
— The Yankees have consistently gone on over the threshold, meaning if they were to do so for '14 the bill could be around $100 million for the next seasons (with each dollar spent over the limit being taxed in excess of 40 percent). The Yanks have long suggested this was the year they didn't want to go over the $189 million, a goal that was fading away after the free agent contracts already given out to Brian McCann, Jacoby Ellsbury and Carlos Beltran.
— With the Yankees now on the south side of the threshold because of the Rodriguez decision, they now have an interesting choice to make: Either they could aspire to stay under $189 million (which would mean no more major signings), or Brian Cashman's club could blow through the threshold and join the Dodgers in becoming baseball's most liberal spending organization in the history of the game.
That leads us to Masahiro Tanaka.
The 25-year-old free agent pitcher's importance in the balance of power in the American League was thought to be all about ability. And that is partially true. Tanaka is perceived as a potential top-of-the-rotation pitcher, the likes of which New York would seem to desperately need.
But it's more than that.
Tanaka is the one player the Yankees would seemingly throw Benjamins to the wind for, leaving the luxury tax in the rearview mirror. He is valued that much. And while New York desperately needs bullpen help, it's doubtful that it would incur such serious financial penalties in order to sign the likes of Grant Balfour. Even free agent starters Ubaldo Jimenez, Ervin Santana and Matt Garza might not be viewed worth storming past $189 million.
But if the Yanks do sign Tanaka, then that could open the door to New York exploring bringing in the likes of Balfour and the rest, filling what are currently a few glaring holds on its roster. The threshold will have already been crossed and belt-tightening won't be the priority. Simply put, once past the $189 million, the dollar diet will probably be done and the free agent buffet will be open once again.
If there is no Tanaka signing, and the Yankees see staying under $189 million as a possibility and priority, they will be left with an extremely thin bullpen and starting rotation that wouldn't seem to match up with the likes of the Red Sox and Rays.
So, other than the chaos that would follow Rodriguez throughout the '14 season, the absence of the player isn't any sort of game-changer for the Yankees. You could even make a case that it makes life more difficult for the Yankees, who not only have the luxury tax decision but are very likely going to have to live through a downgrade at third base. (A-Rod isn't the player he used to be, but he would offer more production than Kelly Johnson.)
This is hardly a get-out-of-jail-free card for the Yankees, the likes of which the Red Sox received via the Dodgers trade in '12. After making the deal with Los Angeles, the Sox freed up $54 million for '13, and another $53 million in '14 without having to worry about anything but how to spend it.
The same can't be said for the Yankees after Rodriguez's suspension.
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Catapult Doreen Flash Game
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Were You There? Reflections on the Final 24 Hours of Jesus' Life
April 13, 2011
The following Easter reflection is by the Rev. Adam Hamilton, founding pastor of the United Methodist Church of the Resurrection in Leawood, Kansas, who will be guest speaker at the California-Nevada Annual Conference's 2011 Annual Conference Session in June. Many of you are likely familiar with Rev. Hamilton's bestselling book, 24 Hours that Changed the World, which is the title after which this article was written.
By Adam Hamilton
When we think of the final day of Jesus' life, it is easy to imagine his suffering. Mel Gibson and a host of others have helped us visualize it in ways the Gospel writers only hint at. What is harder for some is to see themselves in the story. When I wrote 24 Hours That Changed the World, my aim was to see myself in each scene, and to help readers see themselves in each scene as well.
All of us can find ourselves in the story if we'll look closely. Join me for a quick journey through those 24 hours, and try imagining yourself in each scene.
At the Last Supper, 12 disciples enter a room to eat supper with Jesus. Each passes by a basin of water near the door, left there by the owner of the home so Jesus and his guests could wash their feet. Why do they ignore it? Is it possible each is afraid that if he stops to wash his own feet, he'll be expected to wash the feet of the others? None of them wants to be put in the position of a servant washing a fellow disciple's feet. In fact, that night, what is foremost on the minds of some disciples is which of them is the "greatest." (Luke 22:24) How I identify with those disciples as their egos get the best of them! And how easy it is to imagine their shame when Jesus gets up from the table, lifts the basin, and begins to wash their feet.
Before the evening is through, Judas has gone to betray Jesus for 30 pieces of silver, a month's wages. It isn't only greed that motivates Judas. Perhaps his feelings had been hurt when Jesus publicly chastised him. Perhaps his expectations of what Jesus would do have not been met. In a moment of weakness, and for a month's wages, Judas is willing to betray Jesus. I've been Judas, and so has nearly everyone I know. For some, it's money that lures them away. For others, it's sex. Sometimes, it's a desire for freedom. And sometimes, disappointment with an unmet expectation of God makes it easier to walk away. Yes, I've been Judas.
Jesus is arrested late that night in the garden. He is taken for trial before the religious leaders. These are the priests, biblical scholars, and great teachers of their day. All claim to love God, but they don't love this man. He has challenged their theology, blurred the lines between sinner and saint, and said things they consider blasphemous. How do religious people respond when someone challenges their convictions? We often question, judge, and then seek to silence the challengers. We call them names such as "fundamentalist" or "socialist" or "heretic" or "apostate." We sentence them to damnation of one sort or another. Yes, I see myself in the religious leaders who, threatened by this man whose teachings challenge their fundamental convictions, condemn him. Can you see yourself there?
As Jesus stands trial before the Sanhedrin, Peter waits in the courtyard of the high priest, surrounded by people who aided in Jesus' arrest. Three times Peter is asked whether he is a follower of Jesus. Three times he denies it. He's afraid of what might happen to him if, in this hostile environment, he admits he is a follower of Jesus. How many times have I remained silent when others acted unjustly, too afraid to speak out for fear of how many would leave the church or no longer like me? I've been Simon Peter at his worst.
At dawn, Jesus is taken to Pontius Pilate, the Roman governor charged with administering justice. Pilate finds him not guilty of a capital offense. Yet the crowd of merchants, priests, and rabble repeatedly calls for his death. Pilate, "wishing to satisfy the crowd," washes his hands of an innocent man's blood and sends Jesus to be abused and then crucified. How often have we, like Pilate, done what we knew was wrong because we wished to "satisfy the crowd"? Yes, I see myself in Pontius Pilate.
Jesus is taken to be abused at the hands of a Roman cohort of soldiers – 300 to 600 men. They strip him, mock him, crown him with thorns, and strike him repeatedly. Somehow the hate begets hate. Surely some in the crowd know this is wrong. But no one speaks out. I have a difficult time seeing myself among these abusers, though at moments I've felt hatred for those I saw as enemies. I feel more akin to those soldiers who stand by, knowing that what they are doing is wrong but too afraid to speak out.
As Jesus is crucified, those passing by deride him. The religious leaders mock him. Even the thieves being crucified on either side taunt him. Jesus hangs from the cross bleeding, naked, and dying, yet there is no compassion – only the cruelty of words meant to break his spirit. Despite it all, Jesus prays, "Father, forgive them; for they do not know what they are doing." Before long one of the thieves stops his curses and says, "Jesus, remember me, when you come into your kingdom." Jesus turns to the thief and replies, "Today you will be with me in paradise." I want to be that thief on the cross. Like the thief, I've strayed many times from God's path. And, like the thief, when I've finally seen Jesus' mercy and love, I want to put my hope in him.
Where do you find yourself in the story? Do you identify more closely with the actions of the disciples, the religious leaders, the Roman authorities, the soldiers, the crowd, or those passing by?
The last 24 hours of Jesus' life make a compelling case study in the human condition. In that one day, we see greed, betrayal, fear, self-preservation, abandonment, denial, pride, jealousy, anger, rage, mockery, and cruelty. The Sanhedrin think they are putting Jesus on trial, but is it not humanity that is on trial here? The righteous, the powerful, even the disciples are all found wanting. On the cross, humanity is shown to be guilty, but the Son of Man, before whom all humanity one day will stand, suffers in our place. The judge of the "quick and the dead" gives himself for the human race. There, on the cross, he holds up a mirror to our souls; and then, lowering the mirror, he shows us the depth of God's mercy and love.
This is the most compelling, disturbing, redemptive, and hopeful story ever told. It demonstrates to us the truth about humanity and, even more importantly, the truth about God. It points to what is broken in us, and to God's work to save, redeem, and deliver us. But, when Jesus' body is taken down from the cross and placed in the tomb, the story is not finished ...


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Ironwood Distressed Pine Small 40" Rectangular Coffee Tablepp5004090628
web ID: 7941162
The distressed finish of this rustic coffee table gives any space an ample dose of country-style character.
- solid pine construction with a metal frame
- distressed natural finish
- joined with bolts and lock washers
- fixed bottom shelf offers extra display space
- casters allow for easy mobility
Measures 40Wx24Dx16"H. Wipe clean with a soft cloth. Imported.
Rated 5 out of 5 by DebSue325 from Perfect! Hard to find the right size and look for these rustic tables but this was it. Then it was on sale, plus had great additional coupon & free shipping. Epic win! It's a sofa height & not too large for my smaller space. Top it's way cool rustic! My moms on her way over to look & I'll order her one too. Love!
Date published: 2014-07-15
true5 year accent plan $59.99 manufacturer shipped (ships in 1 week(s)),MS1W
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A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: A readout for early mitotic cells
Segrey C.V.,Italian National Cancer Institute |
Senesey S.,Italian National Cancer Institute |
Senesey S.,University of California at Los Angeles |
Lopontey S.,Italian National Cancer Institute |
And 10 more authors.
mAbs | Year: 2016
Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells. © 2016 Taylor and Francis Group, LLC. Source
Hosono Y.,Tohoku University |
Abe T.,The FIRC Institute for Molecular Oncology Foundation |
Ishiai M.,Kyoto University |
Takata M.,Kyoto University |
And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2011
DNA double strand breaks (DSBs) induced by etoposide, an inhibitor of DNA topoisomerase II, are repaired mainly by non-homologous end joining (NHEJ). Unexpectedly, it was found that at high doses of etoposide, proteins involved in NHEJ, such as KU70/80, DNA-PKcs and ARTEMIS/SNM1C, trigger apoptosis rather than repair of DSBs. Because ARTEMIS is a member of the SNM1 protein family that includes SNM1A and APOLLO/SNM1B, this study examined whether SNM1A and/or APOLLO are also involved in etoposide-induced apoptosis. Using SNM1A-/- and APOLLO-/- cells, it was found that both SNM1A and APOLLO participate in etoposide-induced apoptosis. Although cell viability monitored by MTT assay did not differ between SNM1A-/-/. APOLLO-/-/. ARTEMIS-/-, SNM1A-/-/. APOLLO-/-, and single gene knockout cells, DNA fragmentation monitored by TUNEL assay differed between these cells, suggesting that the three SNM1 family nucleases function independently, at least during the induction of apoptotic DNA fragmentation. © 2011 Elsevier Inc. Source
Monterisi S.,Italian National Cancer Institute |
D'Ario G.,Italian National Cancer Institute |
D'Ario G.,Swiss Institute of Bioinformatics |
Dama E.,Italian National Cancer Institute |
And 18 more authors.
Molecular Oncology | Year: 2015
Around 50% of all human microRNAs reside within introns of coding genes and are usually co-transcribed. Gene expression datasets, therefore, should contain a wealth of miRNA-relevant latent information, exploitable for many basic and translational research aims. The present study was undertaken to investigate this possibility. We developed an in silico approach to identify intronic-miRNAs relevant to breast cancer, using public gene expression datasets. This led to the identification of a miRNA signature for aggressive breast cancer, and to the characterization of novel roles of selected miRNAs in cancer-related biological phenotypes. Unexpectedly, in a number of cases, expression regulation of the intronic-miRNA was more relevant than the expression of their host gene. These results provide a proof of principle for the validity of our intronic miRNA mining strategy, which we envision can be applied not only to cancer research, but also to other biological and biomedical fields. © 2014 Federation of European Biochemical Societies. Source
Peleg B.,Weizmann Institute of Science |
Disanza A.,The FIRC Institute for Molecular Oncology Foundation |
Scita G.,The FIRC Institute for Molecular Oncology Foundation |
Scita G.,University of Milan |
Gov N.,Weizmann Institute of Science
PLoS ONE | Year: 2011
Cells exhibit propagating membrane waves which involve the actin cytoskeleton. One type of such membranal waves are Circular Dorsal Ruffles (CDR) which are related to endocytosis and receptor internalization. Experimentally, CDRs have been associated with membrane bound activators of actin polymerization of concave shape. We present experimental evidence for the localization of convex membrane proteins in these structures, and their insensitivity to inhibition of myosin II contractility in immortalized mouse embryo fibroblasts cell cultures. These observations lead us to propose a theoretical model which explains the formation of these waves due to the interplay between complexes that contain activators of actin polymerization and membrane-bound curved proteins of both types of curvature (concave and convex). Our model predicts that the activity of both types of curved proteins is essential for sustaining propagating waves, which are abolished when one type of curved activator is removed. Within this model waves are initiated when the level of actin polymerization induced by the curved activators is higher than some threshold value, which allows the cell to control CDR formation. We demonstrate that the model can explain many features of CDRs, and give several testable predictions. This work demonstrates the importance of curved membrane proteins in organizing the actin cytoskeleton and cell shape. © 2011 Peleg et al. Source
High levels of BRC4 induced by a Tet-On 3G system suppress DNA repair and impair cell proliferation in vertebrate cells
Abe T.,The FIRC Institute for Molecular Oncology Foundation |
Branzei D.,The FIRC Institute for Molecular Oncology Foundation
DNA Repair | Year: 2014
Transient induction or suppression of target genes is useful to study the function of toxic or essential genes in cells. Here we apply a Tet-On 3G system to DT40 lymphoma B cell lines, validating it for three different genes. Using this tool, we then show that overexpression of the chicken BRC4 repeat of the tumor suppressor BRCA2 impairs cell proliferation and induces chromosomal breaks. Mechanistically, high levels of BRC4 suppress double strand break-induced homologous recombination, inhibit the formation of RAD51 recombination repair foci, reduce cellular resistance to DNA damaging agents and induce a G2 damage checkpoint-mediated cell-cycle arrest. The above phenotypes are mediated by BRC4 capability to bind and inhibit RAD51. The toxicity associated with BRC4 overexpression is exacerbated by chemotherapeutic agents and reversed by RAD51 overexpression, but it is neither aggravated nor suppressed by a deficit in the non-homologous end-joining pathway of double strand break repair. We further find that the endogenous BRCA2 mediates the cytotoxicity associated with BRC4 induction, thus underscoring the possibility that BRC4 or other domains of BRCA2 cooperate with ectopic BRC4 in regulating repair activities or mitotic cell division. In all, the results demonstrate the utility of the Tet-On 3G system in DT40 research and underpin a model in which BRC4 role on cell proliferation and chromosome repair arises primarily from its suppressive role on RAD51 functions. © 2014 The Authors. Source


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We believe in looking at your lifestyle choices and making sense out of them with you -- your nutrition, your emotions, your energy, your movement, what you put on your body and in your body -- to help you make a plan to live a fun lovin' life! Young Living Essential Oils is my first product choice to help support you in this wacky, joyful world of wellness. Connect with us here, where we believe in open communication, listening and hearing your own words guide you.
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Gift Certificate Basics:
- Gift certificates are available for 3, 6, and 12 month memberships only.
- Gift certificate codes do not expire. They are valid until they are redeemed.
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- Memberships paid by gift certificate are not automatically renewing. The membership will expire at the end of the billing period and the user will need to rejoin the school (and enter their own payment information) to continue to have access to the school.
Purchasing a gift certificate:
1. Click on "Purchase/Redeem Gift Certificates" at the top of the page, or use this link: http://artistworks.com/gift-certificates
2. Choose the school, select a gift certificate (received by email) or gift card (received by mail), and click "Purchase Now"
3. Select the duration you would like to purchase and click Go
4. Continue to the checkout process, and enter your billing details to complete your order
5. You will receive two emails: one with your receipt/order confirmation, and another with your gift certificate code. The gift code will begin with AWGIFT.
*Alternatively, you can purchase a physical gift card from Amazon.


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Question about RCA M4204 MP3 Player
Posted by douglassamuk on
SOURCE: I have freestanding Series 8 dishwasher. Lately during the filling cycle water hammer is occurring. How can this be resolved
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Users' Perspectives on Implantable Contraceptives for Women
Ortayli, Nuriye
Bibliographic Citation
Contraception 2002; 65: 107-111
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Meet the Barkers
By (author) Tomie de Paola
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Meet the Barkers by Tomie de Paola
Book DescriptionMorgie and Moffie can't wait to start school! They buy backpacks and pencil boxes, and off they go. Moffie, who knows the answers to all of the teacher's questions, gets a lot of gold stars. And Morgie, who likes sharing his dinosaur book, makes two new friends. But by the end of the week Morgie and Moffie help each other to understand that it's important to both get gold stars and make new friends. And they agree that they love school!"
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Book DetailsISBN: 9780142500835
(267mm x 235mm x 6mm)
Pages: 32
Imprint: Puffin
Publisher: Penguin Putnam Inc
Publish Date: 3-Jul-2006
Country of Publication: United States
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Many things happened the year Tomie's family built their new house at 26 Fairmount Avenue, in Meriden, Connecticut, including the hurricane of 1938, Tomie's first day of kindergarten, and more. This is the first in a series of chapter books told from Tomie's perspective as a little boy. A 2000 Newbery Honor Book. Illustrations.
Nana Upstairs & Nana Downstairs, Paperback (January 2008)View all books by Tomie de Paola
Four-year-old Tommy loves visiting the home of his grandmother, Nana Downstairs, and his great-grandmother, Nana Upstairs. But one day Tommy's mother tells him Nana Upstairs won't be there any more, and Tommy must struggle with saying goodbye to someone he loves. Full color.
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calvincollege"We're not solving the problems of this nation that can be solved by tech because we are not filling these jobs with women... We teach our girls to be perfect, and our boys to be brave... Because of that girls go into careers they are good at because they don't know they can do things they would be great at." - Reshma Saujani #JanuarySeries #calvincollege #girlswhocode
- _andrewoppongPreach sister Preach! You tell em!!!! YAAASSS!!!!
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The SpaceX SuperDraco engine has completed its 27th test firing and passed its development tests. The new engine is designed to be flexible, easily adapted, and to save the crew during an abort sequence.
As Alpha Centauri moves away from its companion star, astronomers are training new instruments on the system — hoping to catch sight of an elusive "superhabitable" planet.
Crashing is an ugly word, but you can also call it "lithobraking." Either way, NASA's successful MESSENGER mission to Mercury is about to come to an abrupt end.
NASA and the ESA announced today that the British probe Beagle-2 did indeed land successfully on Mars.
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Investor Relations
This site is designed to provide investors and other interested parties with access to WABCO's important public information.
We invite you to browse through our Investor Center and visit our 2015 Dynamic Annual Report.
If you don't find the information you're seeking, please contact our Investor Relations Contact or Media Contacts.
Thank you.
|$ 114.23
|+1.36 (1.21%)
Day Low: 111.88
Volume: 353,402
Feb 27, 2017
Investor Relations Contact
Christian Fife
Vice President, Investor Relations and Treasurer
Tel: +32-2663 99 58 (Belgium) / +1-248 270 9290 (United States)
Quick Links
Events & Presentations
BRUSSELS, Belgium, January 10, 2017 – WABCO Holdings Inc. (NYSE: WBC), a leading global supplier of technologies and services that improve the safety, efficiency and connectivity of commercial vehicles, will hold its Q4 and Full Year 2016 earnings conference call at 9:00 a.m. Eastern Time on Friday, February 17, 2017. Jacques Esculier, Chairman and Chief Executive Officer, and Prashanth Mahendra-Rajah, Chief Financial Officer, will review the company's financial performance for the period.
The call is also accessible by telephone in listen only mode. Dial-in number is +1 408 940 3818 and U.S. toll-free dial-in number is 877 844 0834.
A replay of the call will be available from 12:00 Noon Eastern Time on February 17 until 12:00 Noon Eastern Time on February 24, 2017. Replay dial-in number is +1 404 537 3406 and U.S. toll-free dial-in number is 855 859 2056. Conference ID is 47675989.