text:
Objective Pigment epithelial-derived factor (PEDF) is a potent anti-angiogenic factor whose effects are partially mediated through the induction of endothelial cell apoptosis. The pathway mediating endothelial cell apoptosis has not been fully established. Here we investigated the participation of peroxisome proliferator-activated receptor δ (PPARδ) and p53 in the apoptosis of human umbilical vein endothelial cells (HUVECs).
Methods and results HUVECs pretreated with either PPARδ antagonist or PPARδ small interfering RNA (siRNA) suppressed PEDF-induced apoptosis as determined by TUNEL assay, annexin V-FITC/PI staining, and cleavage of procaspase-8, -9, -3. PEDF sequentially induced PPARδ and p53 expression as observed in immunoblotting and immunofluoresence assays. PEDF also increased the transcriptional activity of PPARδ as evident from electromobility shift assays, and p53 as determined by the phosphorylation and acetylation of p53 and the induction of Bax. The induction of p53 by PEDF was abolished by either PPARδ antagonist or PPARδ siRNA. PEDF-mediated HUVEC apoptosis and cleavage of procaspases were significantly attenuated by p53 siRNA.
Conclusions Our observations indicate that PEDF induces HUVECs apoptosis through the sequential induction of PPARδ and p53 overexpression. With the growing interest in anti-angiogenesis as a novel approach to cancer therapy, defining the mechanism of PEDF-mediated HUVEC apoptosis may facilitate the development of new therapeutics.
This article is referred to in the Editorial by Gaetano et al. (pages 195–196) in this issue.
The normal vasculature is maintained by a balance between angiogenic factors and anti-angiogenic factors [1,2]. Neovascularization provides a nutrient supply route to the tumor and usually involves a shift towards angioinductive activity [2]. Anti-angiogenic agents can thus be used to fight cancer and other diseases involving neovascularization.
Pigment epithelium-derived factor (PEDF), a 50-kDa secreted glycoprotein, is widely expressed throughout the human body [3], with expression decreasing during human hepatocellular carcinoma and breast cancer progression [4–6], choroidal neovascularization in patients with age-related macular degeneration [7], and diabetic retinopathy [8]. PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown [9,10]. PEDF protects neurons against insults such as glutamate toxicity and oxidative damage and may be a more effective anti-angiogenic factor than angiostatin [3,11]. Systemic injection of recombinant PEDF protein is reported to prevent the development of retinal neovascularization in mice with oxygen-induced ischemic retinopathy by promoting apoptosis of vascular endothelial cells [12].
PEDF exerts anti-angiogenic activity by arresting VEGF- or bFGF-mediated endothelial cell migration [11], inhibiting capillary morphogenesis [13], and inducing endothelial cell apoptosis [12,14–16]. In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase-8, which initiates the downstream apoptotic cascade [14]. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases-8 and-9 [16]. It remained unclear whether Fas signaling is also involved in HUVECs apoptosis and how activated p38 MAPK induced activation of multiple caspases.
Peroxisome proliferator-activated receptor gamma (PPARδ) is a ligand-dependent transcription factor belonging to the nuclear hormone receptor super-family [17]. PPARδ is expressed at low levels in many tissues, including the smooth muscle and endothelial cells of the vessel wall [18]. Exposure of endothelial cells to 15d-PGJ2, a natural ligand of PPARδ, as well as PPARδ overexpression achieved by transient plasmid transfection, each induce caspase-mediated endothelial cell apoptosis [19]. p53 is a well documented pro-apoptotic molecule. It mediates apoptosis primarily via the mitochondrial pathway that induces the release of potent apoptotic activators like cytochrome c[20]. Adenovirus-mediated p53 overexpression in HUVECs has been shown to induce HUVEC apoptosis and inhibit capillary-like differentiation in vitro[21,22]. However, the involvement of PPARδ and p53 in the PEDF-mediated apoptosis has not been studied.
To further our understanding of the mechanism of PEDF-induced apoptosis, we investigated the role of PPARδ and p53 in apoptosis. Our present findings suggest that PEDF activates PPARδ, which in turn leads to overexpression of p53 and apoptosis of HUVECs.
Materials and methods
Cell culture and treatment
HUVECs (Cascade Biologics, Inc., Portland, OR, USA) were grown in Medium 200 with Low Serum Growth Supplement (LSGS Kit, supplement contains 1.9% fetal bovine serum, 3 ng/ml bFGF, 10 μg/ml heparin, 1 μg/ml hydrocortisone, and 10 ng/ml EGF). Culture plates were coated with 2% gelatin. Cells (passages 4−8) were cultured at 37 °C in a humidified atmosphere of 5% CO2. The recombinant PEDF derived from E. coli was prepared as described previously [23]. Treatments with PEDF (200 ng/ml, unless differently specified), PPARδ inhibitors or caspase inhibitors (Calbiochem) were performed on cells (5×105/ml) seeded in LSGS medium.
Evaluation of apoptosis
After treatment, the cells were fixed in 4% (w/v) paraformaldehyde for 16 h at 4 °C and then stained using TdT-mediated dUTP nick-end labeling (TUNEL)-based kit (Roche Molecular Biochemicals) following the manufacturer's instructions. Cell number was monitored by counterstaining with 4',6-diamidino-2-phenylindole (DAPI). The percentage of TUNEL-positive nuclei was calculated in six randomly selected fields of the three different chambers (∼7200 cells). Specimens were examined and photographed on a Zeiss microscope equipped with phase-contrast and epifluorescence optics. Pictures were recorded on Zeiss software. The percentage of HUVECs apoptosis was also confirmed using the Annexin V-FITC Apoptosis Detection kit (Roche). Stained cells were analyzed by flow cytometry (FACScaliber; Beckman).
Western blot analysis
Cells were scraped into lysis buffer (150 μL/35 mm well) containing 20 mM HEPES (pH 7.4), 1% SDS, 150 mM NaCl, 1 mM EGTA, 5 mM β-glycerophosphate, 10 μg/mL leupeptin, and 10 μg/mL aprotinin. Total cell lysate was separated into cytoplasmic and nuclear fractions using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce). Samples containing 20 μg of protein were analyzed by 12% SDS-PAGE and then were electrotransferred to PVDF membranes (Immobilon-P; Millipore) and processed for immunoblot analysis. Antibodies used in this study were for active p38 (Promega), p38/SAPK2, acetyl-p53 (Lys373), caspase-8, caspase-9, and cytochrome c (Upstate Biotechnology), PPARα, PPARβ, and PPARδ (Santa Cruz Biotechnology), p53 (Chemicon), phospho-p53 (Ser15 and Ser20), acetylated-p53 (Lys382), and eNOS (Cell Signaling Technology), cleaved caspase-3 (Abcam), β-actin (Sigma). Proteins of interest were detected using the appropriate IgG-HRP secondary antibody (Santa Cruz Biotechnology) and ECL reagent (Amersham). X-ray films were scanned on the Model GS-700 Imaging Densitometer (Bio-Rad Laboratories) and analyzed using Labworks 4.0 software. For quantification, blots of at least three independent experiments were used.
Semi-quantitative reverse transcriptase (RT)-PCR
Total RNA was extracted from HUVECs with TRIzol reagent (Invitrogen). Synthesis of cDNA was performed with 1 μg of total RNA at 50 °C for 50 min, using oligo (dT) primers and reverse transcriptase (Superscript III, Invitrogen). The amplification mixture (final volume, 25 μl) contained 1×Taq polymerase buffer, 0.2 mM dNTPs, 1.5 mM MgCl2, 1 μM primer pair, and 0.5 U of Taq DNA polymerase (Life Technologies). cDNA was equalized in an 18–22 cycle amplification reaction with PPARδ primers 5′-caggagcagagcaaagaggtg-3′ (forward) and 5′-caaactcaaacttgggctcca-3′ (reverse), yielding a 300-bp product. The number of cycles for the PPARδ primer set (denaturation, 20 s, 94 °C; annealing, 30 s, 57 °C; and polymerization, 40 s, 72 °C) was chosen to be in the linear range of amplification.
Transfection studies
The sequences of PPARδ1 siRNA and control pGL3 siRNA duplexes were synthesized (Dharmacon) as previously described [24]. p53 siRNA was purchased from Santa Cruz Biotechnology. For the transfection procedure, cells were grown to 70% confluence, and siRNA was transfected using TransIT-TKO Transfection Reagent (Mirus Corporation). The final concentration of siRNA was 200 nM. By 24 h after siRNA transfection, cells were resuspended in new culture media, and treated with PEDF.
PPARδ transcriptional activity assay
After treatment, nuclear extracts were collected using a NucBuster protein extraction kit (Novagen), protein concentration was determination by Micro BCA Protein Assay Reagent Kit (Pierce), and the PPARδ transcriptional activity was measured using NoShiftTM II PPAR Transcription Factor Assay Kit (a modified electromobility shift assay; Novagen) as specified by the manufacturer. The specificity of protein binding was established using TransCruzTM Gel Shift PPAR specific and mutant oligonucleotides (Santa Cruz Biotechnology). Luminescence was measured by microplate luminometer (Molecular Devices).
Cells were plated on 2% gelatin-coated coverslips in LSGS medium. After treatment, cells were fixed with 4% paraformaldehyde and then treated at 4 °C with methanol for 10 min, and blocked with 1% goat serum and 5% BSA for 1 h. Cells were stained with antibodies to PPARδ (1:500, Santa Cruz Biotechnology) or p53 (1:1000, Chemicon), incubated with FITC-conjugated goat anti-mouse IgG antibody (1:600 dilution; Santa Cruz Biotechnology) for 1 h, and viewed with an Olympus epi-fluorescence microscope.
Statistical analysis
Data are expressed as mean±standard deviation (SD) of three independent experiments, each done in triplicate (n=3–4 dishes). The Mann–Whitney U test was used to determine statistically significant differences. P values<0.05 were considered significant.
PPARδ antagonist abolishes PEDF-induced HUVEC apoptosis and caspase activation
To examine the involvement of PPARδ in the signaling of PEDF-induced HUVEC apoptosis, cells were pretreated with PPARδ antagonists (GW9662 or T0070907; 10 μM, 1 h) and then exposed to PEDF for 24 h. The apoptotic cells were assayed by TUNEL staining. As shown in Fig. 1A, both PPARδ antagonists can markedly inhibit PEDF-induced apoptosis. Pretreatment with the broad spectrum caspase inhibitor, zVAD-fmk (20 μM, 1 h), inhibited the PEDF effect, indicating caspase mediation. Quantification of apoptosis by annexin V-conjugated FITC and propidium iodide (PI) staining and fluorescence activated cell sorting (FACS) showed that treatment of HUVECs with PEDF for 24 h increased late apoptotic cells from 3.3±0.6% to 17.2±1.9% and pretreatment with GW9662 suppressed PEDF-induced apoptosis to ∼4.8±0.9% (Fig. 1B, upper right). Thus, inhibition of PPARδ activity attenuated PEDF-induced HUVEC apoptosis.
PEDF-mediated HUVEC apoptosis is associated with cleavage of procaspase-3, -8 and -9 [16]. Pretreatment with the caspase-8 inhibitor or caspase-9 inhibitor (20 μM, 1 h) partially inhibited PEDF-induced apoptosis (13.5±1.8% or 7.1±1.5% versus PEDF+DMSO; 17.2±1.9%). The PEDF-induced apoptosis was almost completely blocked by pretreatment with both inhibitors combined (Fig. 2A). To investigate whether Fas-FasL death cascade is involved in PEDF-induced HUVEC apoptosis, FasL neutralizing antibody (NOK-2) was employed. As shown in Fig. 2B, NOK-2, which effectively blocks PI3/Akt inhibitor (25 μM LY-294002)-induced Jurkat cell apoptosis as previously reported [25], could not protect HUVECs from PEDF-induced apoptosis. This suggests that PEDF-induced HUVEC apoptosis is Fas-independent. Western blot analysis also revealed that treatment with PEDF but not PEDF solvent for 16–24 h significantly increased levels of cleaved caspases-8, -9 and -3 (Fig. 2C). Cytochrome c (Cyt-c) release from mitochondria to the cytosol has been shown to promote caspase-9 activation during apoptosis [20]. Western blot analysis showed a significant increase in Cyt-c release after PEDF treatment for 14 h (Fig. 6A). These studies confirmed that activation of both caspase-8 and -9 is involved in PEDF-triggered apoptosis and that no increased procaspase-8 and -9 cleavages occur at 1–12 h of incubation with PEDF (data not shown). Importantly, the PEDF-induced procaspases cleavages and Cyt-c release were markedly inhibited by pretreatment with GW9662.
PEDF induces PPARδ expression and activity
To investigate whether PEDF exposure can induce PPARδ expression, time-course analysis of PPARδ mRNA by RT-PCR was performed and revealed that the level of PPARδ mRNA was increased at 2 h, peaked at 4–6 h, and then dropped at 8 h as compared with an untreated or solvent-treated control (Fig. 3A). When HUVECs were pretreated with actinomycin D for 1.5 h or 3 h prior to PEDF exposure for an additional 6 h, the PPARδ mRNA level was suppressed (Fig. 3B), suggesting the increase of PPARδ mRNA was transcription dependent. The influence of PEDF on PPARδ protein expression assayed by immunoblotting revealed increased expression at 4−8 h and peak expression at 6–8 h as compared with solvent-treated cells (2.7±0.4 fold; after treatment for 6 h; Fig. 3C). Western blots also revealed expression of both PPARα and PPARβ in HUVECs, but PEDF treatment had no effect on their levels (Fig. 3C). Immunofluorescence analysis of PPARδ localization showed nuclear accumulation of PPARδ protein in untreated cells but increased nuclear and cytoplasmic accumulation of PPARδ protein after treatment with PEDF for 6 h (Fig. 3F). Thus, PPARδ expression was upregulated by PEDF at both the mRNA and protein levels.
A recent report indicated that PEDF induces p38 MAPK-dependent HUVEC apoptosis [16]. As shown in Fig. 1A, pretreatment with the p38 MAPK inhibitor, SB203580 markedly abolished PEDF-induced HUVEC apoptosis. In addition, PEDF stimulated a ∼3 fold increase in p38 MAPK phosphorylation for intervals ranging between 15 and 25 min (Fig. 1C). Therefore, our results confirmed the involvement of p38 MAPK. To investigate the role of the p38 MAPK signaling in PPARδ expression in HUVECs, SB203580 was also tested. RT-PCR analysis revealed that pretreatment with SB203580 completely blocked PEDF-induced PPARδ expression, whereas pretreatment with PD098059 (ERK inhibitor), SP600125 (JNK inhibitor), or DMSO had no such effect (Fig. 3D). Western blot analysis also revealed that pretreatment with SB203580 (but not PD098059 and SP600125) significantly blocked PEDF induction of PPARδ (Fig. 3E). Therefore, we suggest that PEDF induces p38 MAPK activation to upregulate PPARδ expression in HUVECs.
To investigate whether the PPARδ transcriptional activity could be increased after PEDF induction of PPARδ expression, a modified electromobility shift assay was employed to investigate the interaction between PPARδ and its responsive DNA. We found that the exposure of cells to varying concentrations of PEDF for 6 h induced a dose-dependent increase of DNA binding of PPARδ (Fig. 4) suggesting enhancement of PPARδ transcriptional activity. To identify the PPARδ isoform responsible for this effect, HUVECs pretreated with the PPARδ specific inhibitor GW9662 (10 μM, 1 h) before PEDF treatment. The suppression of the DNA binding effect indicates the major binding factor is PPARδ.
PPARδ induction is critical for PEDF-mediated apoptosis
The above results suggest that PPARδ mediates PEDF-induced apoptosis (Figs. 1 and 2B). To further establish this notion, HUVECs were transfected with PPARδ-specific siRNA before PEDF treatment to prevent PPARδ expression. RT-PCR and Western blotting verified that transfection with PPARδ siRNA (but not control siRNA with a sequence unrelated to PPARδ) abolished PEDF induction of PPARδ (Fig. 5A and B). Importantly, compared with control siRNA, PPARδ siRNA significantly reduced PEDF-induced cleavages of procaspases (Fig. 5B) and apoptosis (Fig. 5C). This confirms that PPARδ expression is required for PEDF-mediated apoptosis.
PEDF induces p53 gene expression and activates p53-mediated transcription through PPARδ
PPARδ can upregulate p53 gene expression [26]. We performed Western blot analysis to find out whether p53 protein level is increased by PEDF treatment. p53 levels were increased at 6 h after PEDF exposure and peaked at 10–14 h. Moreover, GW9662 pretreatment blocked p53 induction of PEDF (Fig. 6A). Transfection of HUVECs with PPARδ-specific siRNA attenuated PEDF-induced p53 overexpression (Fig. 6B and C), confirming the involvement of PPARδ in p53 induction. Immunofluorescence analysis of p53 localization showed nuclear accumulation of p53 protein before PEDF treatment and increased nuclear and cytoplasmic accumulation as compared with untreated control after treatment for 12 h (Fig. 6D). Thus increase in both PPARδ level and activity is essential for the induction of p53 expression. Endothelial nitric oxide synthase (eNOS) activation can be a candidate mechanism mediating endothelial apoptosis [27]. However, PEDF exposure did not change the levels of eNOS protein (Fig. 6A)."
Phosphorylation and acetylation of p53 are important for its promoter targeting and influence on cell fate [28,29]. To confirm that the PEDF-induced p53 is transcriptionally active, we investigated posttranslational modifications of p53. As shown in Fig. 7, PEDF stimulation caused increase in p53 acetylation (K373 and K382) and phosphorylation (S15 and S20) in a time-dependent manner, in parallel with PEDF induced increase in the expression of total p53, indicating the overexpressed p53 is modified. Since Bax is a p53 target gene and a marker of p53-mediated proapoptotic effects [28], we investigated whether Bax is induced by PEDF stimulation. As shown in Fig. 7, PEDF treatment induced Bax expression accompanying p53 overexpression.
Taken together, our results indicate that PEDF upregulates p53 protein expression and transcriptional activity in HUVECs and these PEDF effects are mediated by PPARδ.
PEDF triggers p53-dependent HUVEC apoptosis
To verify that PEDF-induced p53 overexpression is required for PEDF-mediated apoptosis, HUVECs were transfected with p53-specific siRNA before PEDF treatment to prevent p53 expression. RT-PCR and Western blotting verified that transfection with p53 siRNA abolished PEDF induction of p53 (Fig. 8A and B). Western blot analysis also revealed that the ability of PEDF to induce procaspase 8, 9 and 3 cleavage was significantly suppressed in p53 siRNA-transfected HUVECs as compared with mock or control siRNA-transfected cells (Fig. 8B). Quantitative analysis of the numbers of TUNEL-positive cells revealed that PEDF-induced apoptosis of p53 siRNA-transfected HUVECs was significantly lower than control siRNA-transfected cells (Fig. 8C, P<0.01). Thus, p53 level also controls PEDF-induced apoptosis.
Induction of apoptosis of proliferating endothelial cells is an important PEDF anti-angiogenic activity. However, apoptosis regulation by PEDF signaling remains poorly understood. Here we used HUVECs to investigate proapoptotic inducers of PEDF signaling. We demonstrated that PEDF sequentially induces PPARδ and p53 expressions. Blocking any one of these effects caused attenuation of apoptosis. Use of proapoptotic inducers of endothelial cells may provide a new therapeutic avenue to restrict pathological neovascularization.
Our present study is the first to report the participation of PPARδ in PEDF signaling. We found that PEDF increase the expression (Fig. 3) and transcriptional activity (Fig. 4) of PPARδ in HUVECs. To establish the essential role of PPARδ in PEDF effect, we employed PPARδ-specific siRNA and PPARδ antagonist to inhibit PPARδ signaling and demonstrated the prevention of apoptosis (Figs. 1 and 5C) and caspases activation (Figs. 2C and 5B). Interestingly, the capability of PPARδ to induce endothelial apoptosis has been demonstrated in a previous report that exposure to PPARδ agonists or transfection-mediated overexpression of PPARδ can stimulate endothelial apoptosis [19]. The connection between PEDF and PPARδ is less clear. However, a recent report indicated that PEDF receptor is a phospholipase A2 (PLA2)-linked cell membrane receptor [10], while PLA2 can enhance the formation of PPARδ ligands to activate PPARδ in vascular endothelial cells [24]. These observations provide possible signaling mechanism between PEDF and PPARδ and support our model that PEDF signals through PPARδ.
Recently, PEDF-mediated HUVEC apoptosis was reported to be associated with activation of p38 MAPK [16]. We further found that treatment with the p38 MAPK inhibitor extensively blocks PPARδ mRNA and protein expression (Fig. 3) indicating that p38 MAPK is upstream to PPARδ in PEDF signaling. The mechanism of p38 MAPK-mediated PPARδ expression remains elusive. It has been found that early growth-response factor-1 (Egr-1) acts as transcriptional activator of PPARδ1 gene [30]. Whether p38 MAPK can regulate Egr-1 activity to promote PPARδ expression in HUVECs awaits further investigation.
Our study is the first to demonstrate that p53 is a mediator of PEDF-induced apoptosis (Fig. 8) and to reveal that PEDF upregulates p53 expression via PPARδ (Fig. 6A and B), identifying p53 as a major target in PPARδ-mediated apoptosis. The demonstration that PPARδ induces p53 expression by binding directly to the promoter region of p53 gene in human MCF7 breast cancer cells [26] suggested that PEDF might induce a similar transcriptional response in HUVECs. However, our results cannot rule out that PPARδ may trigger entry into a proapoptotic state by repressing the expression of survival/anti-apoptotic genes in HUVECs. In that report, association of nuclear receptor corepressor complexes with PPARδ caused NF-κB promoter silencing [31]. NF-κB expressed in ECs is considered important for resistance to many apoptotic stimuli [32]. The potential role of NF-κB in PEDF-mediated cytotoxicity can be tested experimentally.
Previous reports show that PEDF generates anti-angiogenic signals by activating the Fas-FasL death cascade. This effect is prevented in ECs by treatment with neutralizing antibodies against FasL or is absent in mutant mice defective for Fas or FasL [14,33]. Fas-mediated signaling mainly induces procaspase-8 autoproteolytic cleavage, although it may subsequently cause activation of procaspase-9 [34]. Our finding revealed that caspase-9 inhibitor provides more extensive protection than caspase-8 inhibitor from PEDF-induced apoptosis (Fig. 2A). In addition, we found that the FasL neutralizing antibody could not protect HUVECs from PEDF-induced apoptosis (Fig. 2B). These suggest that PEDF-induced apoptosis in HUVECs is Fas-independent and occurs primarily through activation of procaspase-9. In support of this notion, PEDF was found to inhibit ocular angiogenesis in mice deficient in Fas or FasL [35] and induces caspase-9 activation in HUVECs [16]. However, our data do not rule out differences in Fas signaling between HUVECs and ECs from other sources.
Expression of PEDF (an important multifunctional factor) decreases during progression of many diseases. We are the first to show that PPARδ and p53 acts as an apoptotic activator in PEDF-mediated signaling. This finding may contribute in part therapeutic solutions for conditions such as cancer. In addition, the pleiotropic effects of PPARδ and p53 on different cell types may help to explain the multiple biological functions of PEDF.
We thank Ju-Yun Wu for technical support. This study was supported by grants from the National Science Council, Taiwan (NSC 95-2314-B-195-009-MY3, NSC 96-3112-B-195-001) and Mackay Memorial Hospital (MMH- E - 96006).


text:
A pack of holographic, peelable and reusable foil stickers, which feature a variety of different species of insects. They are suitable for craft projects or decorating stationery, as well as being ideal party bag fillers.
Thank you for visiting Insect Stickers on shoppingfind.co.uk
We bring the products from many websites to one website for you to search, save and compare.

subset: commoncrawl


text:
Keep strawberries fresh and firm with this pod from Joie. Crafted from BPA-free plastic, it provides ventilation and protection but can also be used to rinse, strain and serve other berries as well as strawberries.
Thank you for visiting Joie Strawberry Storage Pod on shoppingfind.co.uk
We bring the products from many websites to one website for you to search, save and compare.


text:
The I've Been on the Internet Test
I Built the Net
To you, the internet is a tool. A tool to get the most degraded garbage you can.
You don't even do the nice things on the net! You just go straight for the garbage! What is this obsession? What's wrong with you?
You know, it's people like you who built the internet. Sickos. Porn-obsessed weirdos. I bet you're sitting on a fat wad of cash from all your cam girls and Nigerian businessmen scams, aren't you?
All possible test results
You might have been on this big ol' internet, but you haven't seen the half of it! And if you're a halfway decent person, after doing this test you probably don't want to see anymore. Y... Read more
You haven't seen much internet, but you've had a good, long cry. That makes you a goth. In the small amount of things you've seen on the web, almost all of them seem to be sad things. L... Read more
How did you even get into this category? It should be impossible to do!!! No really! You can't have done all the sadest stuff on the internet without DOING the sad stuff. If you ... Read more
Site Seer
You've done all the fun stuff. You've seen all the freebies. You're an A-OK internet type person. With all the possibilities online, you've stayed a true path. You don't do degrading or... Read more
Average Netter
Congratulations! You're average. You've been to plenty of places and done plenty of stuff. Some of the stuff is a little sad, probably your LiveJournal is what's letting you down. But d... Read more
I Built the Net
To you, the internet is a tool. A tool to get the most degraded garbage you can. You don't even do the nice things on the net! You just go straight for the garbage! What is this obsessi... Read more
Net Nanny
You're a master of the internet. You do everything you can. That is, of course, without doing anything nasty. You've managed to suck the maximum amount of good, clean fun out of this ol... Read more
Old Hand
And you thought you'd seen it all! You've been around this old internet of ours a number of times. You've seen some pretty sick stuff, and maybe you even liked some of it, but you've ma... Read more
You're becoming a wierdo. Is this really where you want to go? You've managed to see a whole lot of tears, but you haven't seen everything just yet. I bet you're curious as to wh... Read more
Holy Mother
Did you seriously get into this category? I didn't think it was possible. How did you manage to miss all the tears yet see all the stuff? Please, please message me and let me know. You'... Read more
Congratulations! You're the internet master. You've managed to see just enough of it without turning yourself into a monster. And don't go any further! It's dehumanizing from here on in... Read more
Colonel Kurtz
I've seen horror... horror that you've seen. You've seen it all. This is just another pitiful test in your long line of degraded porn, sub culture horror movies and snuff footage. The i... Read more
Tests others are taking
Ginger-skies24 / m / Straight
The Sidekick One Question Test
Simplytashie23 / f / Straight
The Nonrepresentational Aesthetics Test
Justin-Jzx26 / m / Straight
bollzye25 / m / Straight
CLS85197442 / f / Straight
More tests we think you'll like
Connect your existing OkCupid account
Sign in
Welcome Back!
Restore your account to continue meeting new people.
Reset your password
We'll email you a link to reset it.
Email sent!
An email is on its way to .
If you don't see it, try checking your Spam folder.
Enter Authentication Code
Sign in
Not so fast!
You need to complete your basic info on the mobile app before you can use the desktop site.


text:
Cheyenne is a Friesian/Appaloosa mare offered for sale in Lakeville, MN. Rides English and Western. Likes to jump, has NICE movement and likes to go somewhere…SOLD
Looking for a horse that does it all? Easy is your guy! -Name is Easy -Registrable, 5 year old, Smutty Buckskin, Gelding -Has been used for lessons…SOLD
For Sale: Oldenburg/Morgan filly. 2 years old and will dapple out. Minimal handling and needs experienced horse owner! Must free up a stall ASAP. Make…SOLD
This is an exceptional 4 year old gelding. He is a FFA project for a 14 year old young man. He is still in training but you can take him on the trail…SOLD
"MOONIE" is and exceptionally well put together mare!! She is broke, broke, broke!! Ponied colts, checked miles and miles of fence, just a great all…SOLD
Two Registered Half Sisters Yearling Fillies For Sale. Foaled in spring of 2012 out of the same sire. Located in Princeton, MN $450 each, $650 if…SOLD
"Jet" is an absolutely stunning mare by our stallion Little Haidas Lena x the legendary Haidas Little Pep and out of a daughter of Gay Bar Lena x the great…SOLD


text:
TW Racquet Experts Selecting the right racquet and string for your game can be a difficult task. When you consider the tremendous amount of racquets available, and pair them with the even larger selection of strings on the market, you are looking at a mountain of combinations! Here at Tennis Warehouse, we want to make the selection process a bit easier for you. That's why we have a talented group of Racquet Experts on staff to help each player find the right racquet and string combination for their play style. Call or email us with your questions, and a Racquet Expert will be happy to point you in the right direction!
Toll Free: 1.800.799.9230
Email: firstname.lastname@example.com
Hours: M-F 6am-6pm, S/S 9am-6pm
One of the best options if you are looking to dominate the court with power and spin, the Pure Drive Plus offers tremendous access to both. From the baseline, the racquet provides extra leverage to maximize power. The crisp response and open string pattern result in excellent ball speed off the face of the racquet. Both topspin and slice can be taken to new levels with this one. Feel from all areas of the court is improved with Babolat's Active Cortex technology. The result is impressive comfort and a more dialed in response on touch shots. One of the best racquets for powerful serving, our TW playtesters also found impressive consistency with this one thanks to the added spin which enabled them to control the power. Add your favorite multifilament string to further improve power and comfort, or your favorite co-polyester to take control and spin to the next level and you can tailor this one to taste. A great option for singles and doubles players, this versatile racquet manages to be the perfect fit for intermediate players all the way through to the pro tours. Note: Due to racquet stiffness, when stringing with a polyester or a co-polyester we highly suggest 54lbs as a starting point for tension.
|Head Size:
100 sq. in. / 645.16 sq. cm.
|Length: 27.5in / 69.85cm
|Strung Weight: 11.2oz / 317.51g
|Balance: 5 pts HL
|Swingweight: 317
|Stiffness: 70
|Beam Width: 23.5mm / 26mm / 22.5mm /
|Composition: Graphite
|Power Level: Low-Medium
|Stroke Style: Medium-Full
|Swing Speed: Medium-Fast
|Racquet Colors:
|Grip Type: Babolat Syntec
|String Pattern:
16 Mains / 19 Crosses
Mains skip: 8T 8H
Two Pieces
No Shared Holes
|String Tension: 50-62 pounds
Volkl Organix 7 (310g)
Sale: $99.00 Was: $189.99
Buy 2 or more for $89.00 each.


text:
Synaptic plasticity is essential for the development of brain, learning and memory. Hebbian-type plasticity such as long-term potentiation and long-term depression is rapid and synapse-specific modification. In contrast, homeostatic plasticity involves global modification of synapses, operates over longer timescales, and is believed to be crucial for the maintaining and orchestrating neuronal network function. Hebbian-type plasticity is mediated mainly by the trafficking of AMPA receptors but not much is known for the mechanisms of homeostatic plasticity. Recently, activity-dependent protein turnover at the synapses by ubiquitin-proteasome system has emerged as crucial mechanisms associated with various types of synaptic plasticity including homeostatic plasticity. However, it is unknown how activity orchestrates concomitant ubiquitination/degradation and recruitment of specific group of proteins at synapses. Among the activity-regulated proteins, GKAP is one of the major scaffolding proteins in the postsynaptic densities and provides a molecular link for PSD-95/NMDA receptor complex and Shank/Homer. Our preliminary studies suggest that activity controls the recruitment and removal of GKAP from synapses, both through Ca2????dependent protein kinase II (CaMKII). Further, we found that the activity-dependent turnover of GKAP is required for synaptic scaling in hippocampal neurons. In this proposal, we will investigate the molecular mechanisms by which CaMKII controls ubiquitination/degradation or recruitment of GKAP to synapses, and the functional significance of the GKAP turnover at the synapses in various types of synaptic plasticity.
Aim 1 will map the CaMKII phosphorylation site(s) and ubiquitinated lys site(s) that induce ubiquitination of GKAP, using a combination of mutagenesis and biochemical assays.
Aim 2 focuses on understanding the role of DLC, MyoV, and CaMKII for GKAP recruitment to synapses by molecular genetic approaches. We will also perform real-time imaging to understand dynamic GKAP trafficking with greater spatio-temporal resolution.
Aim 3 will assess the functional significance of GKAP removal/recruitment at synapses for the activity-dependent modification of synapse compositions and various forms of synaptic plasticity, by using GKAP mutants lacking the activity- dependent turnover. Since aberrant synaptic plasticity is implicated for a variety of neurological and neuropsychiatric diseases, the proposed studies will not only allow us to gain novel and fundamental insight into the molecular mechanisms for long-lasting changes in synapse compositions but also are relevant to these brain diseases.
Synaptic plasticity is a fundamental mechanism by which neurons store experience and forms a foundation for learning and memory. The main subject of this research project, GKAP, is implicated for number of neurological diseases including autism, schizophrenia, and obsessive-compulsive disorder. Thus, studying the function of GKAP in synaptic plasticity not only help understanding the higher cognitive function of human but also is directly relevant to disease.


text:
Welcome back, lovers of dysfunction, willful wallowers and aficionados of absolute disgrace – Showtime's trashcan opera Shameless is back for a third season of laughs, thrills, and all the rest. I hope that you put on your chin-pad, because your jaw is going to start hitting the floor in about 3 … 2 … 1 …
Fiona (Emmy Rossum) wakes alone in her charmingly messy bed to the sounds of standard Gallagher family chaos downstairs. Her shins are covered in red, itchy bites – yowch! Are bedbugs the new Karen Jackson? Because I don't think I'm ready for that.
Downstairs, Jimmy-nee-Steve (Justin Chatwin) has gotten half the clan out the door and is finishing up with Carl (Ethan Cutkosky) and Debs (Emma Kenney) – who has been keeping track of the number of days that Frank has been missing (oh – and Frank has been missing) with ribbons and cards on the front fence. For the record, it's been 137 days. Fiona and Jimmy bicker about money and who loves the kids more (at least some things never change) and almost do it in front of wee baby Liam.
We then catch up with Frank (William H. Macy) as he is licked awake by a dog in some hot, dusty alley. He's in Mexico, and has no idea how he got there, or how he managed to miss Christmas. Shame – I hear your family roasted a bald eagle!
We then catch up with Lip (Jeremy Allen White) and Ian (Cameron Monaghan) as they flee (with security in hot pursuit) the university science building toting a metal suitcase. Lip takes the fall to let Ian escape, as he knows a rap sheet will mess with his military training. Aww!
Sheila (Joan Cusack) and Jody (Zack McGowan) are enjoying domestic bliss with Karen's baby (which they stole) – meaning that neither of them is getting any sleep. The baby cries and Sheila tries to get Jody to attend to it, finally screaming, "Get your ass up, you goddamned ponytailed Fabio motherf*cker!" It does the trick – and the baby stops crying until Jody starts playing the bongos to it, which makes it cry even harder. Bless his stupid ponytailed heart!
Frank hitches a ride to the US/Mexico border on the back of a chicken truck, and tries to use an egg as a passport to make his way back in. The border police isn't having it, telling him that he needs some sort of ID, be it a driver's license or Costco card. Frank replies, "Do I look like I got a Costco card?"
Back at the Gallagher compound, Fiona is washing her face in the bathroom when Jimmy barges in to pee and then "wash" his hands in her beauty regimen water. Men! Amiright, guys? [imaginary high-five]
Fiona goes over to complain to Veronica (Shanola Hampton) and Kev (Steve Howie), who are getting ready for a live webcam show. First off, let's all celebrate the fact that Steve Howie is back in fighting form this season, eh? Even with a broken leg. And let's all contemplate the magnificent wrongness of their webcam concept: it's a slave owner/slave BDSM revenge scenario. More on that later.
Fiona's new gig is cleaning up toxic waste, apparently, and yet she's upset that Jimmy has taken such a shine to being a househusband. V agrees: "There is nothin' sexy about a man with a mop." I don't know what universe these women live in, but it's apparently one without the Brawny Man.
At home, Jimmy is very, very nervous about the cars that seem to be parked outside at all times watching the house. He decides to confront them, and is immediately shaken down by … Estefania's dad? Uh-oh.
Lip shows uncharacteristic maturity at his hearing, and the judge points out that he's lucky he's still a minor.
Fiona, in a hazmat suit, steps into the filthiest bathroom this side of Trainspotting. Seriously, it's absolutely vile – and I may never eat Nutella again. She also misses a collect call from Frank, who is dialing for dollars in Mexico. He also tries Sheila – she initially gets excited because she thinks it's Karen, but when she hears Frank's voice she hangs up the phone.
Estefania's dad drags Jimmy over to their happily-married apartment, where Marco (Thierre Di Castro) – gorgeous, mean, corkscrew-shy Marco – struts out of the bathroom wearing only headphones and strawberry lube.
They shoot him. A lot.
Estefania (Stephanie Fantauzzi) screams. A lot.
Frank joins up with a coyote – a guy who helps smuggle people over the border into the States. This is gonna end well.
Ian and Mandy bail Lip out (well, Mandy, mostly – her family gets a bulk discount at the bail bondsman, apparently) and Ian learns that she hasn't seen Pig Pen Mickey recently, although he has asked her for more girl-on-girl porn, which is all he ever wants. Interesting?
Back at Estefania's house, her dad makes Jimmy help him and his crew cut Marco into pieces with a power saw. YIKES. Remember last season's boat party episode? I made a crack that it was like a crossover with Dexter just waiting to happen. Go ahead and double that. Her dad also tells Jimmy that he's taking his daughter – Jimmy's wife – to dinner.
Fiona gets fired because her boss – who is eating a bean burrito that looks to be composed of exactly the stuff that they were just knee-deep in – has to hire her nephew for the summer. Bummer!
Under the overpass, Lip is setting up whatever it is he stole from the university – it looks to be a laser? Oh, this is gonna end well…
Later, we see him attaching it to the top of a mini Mars rover.
Ian heads out for "study group". Fiona's concerned that Jimmy isn't at home – mostly because it means that nobody fed the kids. Wait, wasn't she just complaining that he's home too much? Ladies! Amiright, ladies? [imaginary chest-bump]
Debs – who has seriously grown between seasons – lights a candle for Frank. Fiona assures her: "Don't worry about him. Frank's like scabies." And apparently she'd know! Fiona then sees something out back and goes outside to find Jimmy, in his undies, burning his clothes. She doesn't ask and he doesn't offer, but he does throw his undies on the fire for good measure, and then invites her to take a shower with him.
Speaking of Frank, he's the most annoying border-jumper that these coyote guys have ever seen. But before they can shoot him and leave him for the vultures, they're ambushed by immigration officials. Frank gets chased and caught.
Ian is getting "tutored" by Lloyd (Harry Hamlin), whom he now knows happens to be Jimmy's father. I'm going to just have to go ahead and create a keystroke shortcut for "This is gonna end well."
Lloyd gives Ian a GPS thingie to help out with his military training, but Ian refuses the gift, mumbling, "I sort of have a boyfriend." Lloyd responds, "I sort of have a wife." Touche! He then asks if Ian and his fella would ever be up for a threesome. See? Now that's the spirit – take a potential negative and turn it into positive. When life gives you jailbait with a boyfriend, make jailbait-ade.
Also, Cameron Monaghan has certainly grown up fast, hasn't he? What are they feeding these children? Oh, right – bald eagle.
Lip's hanging out in the school's metal shop with Mandy, and he's making… something metally. The guidance counselor pops in to tell Lip to spend his summer doing something productive to pad his resume, and Lip asks why everyone thinks he's going to college. Mandy seems surprised to learn that Lip is a genius. Given her 1.2 GPA, this isn't a shocker.
Fiona is back at Kev and V's, moaning about how she can't find a job. V's getting ready for another webcam show, and asks, "Is this Aunt Jemima scarf too much?" No, V. No, it's not. V advises against any job for Fiona that involves a uniform: "Everyone looks like they have a d*ck in those pants!" She and Kev then get down to the business of show: he is Thomas Jefferson (oh sweet Lord…) and she is Sally Hemings, who is whipping him for raping her in the barn. "We shall overcome, motherf*cker!"
Fiona decides to revisit the club she used to work at, where the manager once offered her an assistant manager gig (but Fiona blew it). Meg (said manager) – who is now pregnant – isn't feeling it, but she tells Fiona that she will give her a night to promote if Fiona gives her $1000. Wait, what? Is that how that business really works? Later, V lends Fiona the cash (their webcam show was a big hit!) and gives her a pep talk about how she's Fiona Gallagher and she can do anything. Fiona goes back to Meg – she's reluctant to take the cash because of Fiona's family obligations, but she does.
Jimmy, meanwhile, is on Dexter Morgan's Slice of Life, and his father-in-law is eating pounds of exotic fruit and throwing parts of Marco's exquisite body into the sea. Estefania's dad – while skeet-shooting watermelon with a machine gun, I might add – tells Jimmy that he's going to be the husband to his daughter that he promised to be. Jimmy needs to keep his nose clean, though, because he wants his daughter to become an American citizen. This means getting a job. He does give Jimmy permission to continue "f*cking that ghetto whore." Manners, pal! It's "making love to that ghetto whore."
Frank begs for help and is taken to a guy who looks sort of like someone hit Bruce Campbell in the face with a shovel and then drew on a goatee. They yank down Frank's pants, force him to bend over, and check out his sun-don't-shine. Oh dear, I think I know where this is going – and not because it vaguely resembles most of the dates I had in my twenties. They issue Frank a fake passport and Flat-Face Bruce Campbell brandishes a massive lozenge of cocaine. Frank balks: "There's no way I can swallow that!" They never said anything about swallowing it … or the other three that go with it.
Turns out Lip is building a robot to enter into a Robot Wars-style battle competition. Okay, this is awesome. The College Douchebag running the show recognizes Lip and tries to block his entry ("We don't take food stamps.") but Lip gets his 'bot in, with Ian and Mandy's help. It initially looks like Lip's rather sad-looking robot (which draws jeers from the crowd of generic nerd-types) is going to get trounced by College Douchebag, but the Lip activates the laser and blows the other 'bot the eff up.
Are we all on the same page here, folks? The Gallaghers have WORKING LASERS now. Hide your kids, hide your wife.
Frank makes it past the border guard who bounced him the first time, and he's walking like he has a bowling ball in his pants. (Or a bowling-ball-sized eightball.) He's greeted on the other side by a grinning girl in a convertible who tells him that he's already earned the nickname "El Grand Canon" – because while the record for ass-coke-balloons used to be eight, Frank stashed a full EIGHTEEN. He replies, "It's like taking a sh*t backwards." Well, it's actually like taking EIGHTEEN SH*TS backwards, but who's counting? (Not the drug dealers, apparently – more on that later.)
Fiona nearly catches Carl watching Kev and V's web show (he says he's watching The History Channel), and Lip and Ian come in bearing a trophy and the $400 cash prize from the Nerd Olympics. He gives it to Fiona to put toward the property taxes, which have been hanging over her head the whole episode. The gang gathers for dinner, and Debs insists that they save Frank's seat.
Sure enough, Frank shuffles up the walk, chortling at Debs's display on the front fence. He steps inside and watches the gang talk animatedly around Jimmy's big bowl of spaghetti. It actually looks for a moment as though he may not go in – but then one by one the kids spot him in the doorway and fall silent. Debs is the only one who welcomes him back – as she rushes to hug her dad, the rest of the kids go back to talking and eating.
Later, we see Frank taking a crap, which is every bit as delightful as it sounds. He gives himself a half-assed wipe (pun intended), and then turns, reaches into the bowl and plucks out a big ol' coke balloon. He smiles: "Nineteen," and then adds, "Thank you, Sheila!" Wait, wha- OMG, because she spent so much time breaking him in with those massive dildos of hers. Okay, that's f*cking brilliant.
Not bad! It felt a bit like coming home, didn't it? That is, if your home was being cased by Brazilian drug lords, you were housing a girl for whom you recently threw an abortion fundraiser, and your kids were building FRIKKING LASERS at the kitchen table. V and Kev continue to amaze me, I'm cautiously optimistic that Lip might get his shizz together and step up as man of the house, and Ian … well, let's just say that I'm enjoying fully-cooked Cameron Monaghan a little more than I'm willing to admit. Big, round thanks to an alarmingly tan Justin Chatwin for delivering the rump roast this week, and let's all raise eight out of ten Old Styles to dearly departed studmuffin Marco:


text:
Hope everyone had a nice Christmas and looking forward to a great New Year. James was sick so we did not get to attend Christmas dinner at his niece, Amanda Medley's and the Eastern Gate program. Christmas Day we enjoyed ...Read More »
I hope the woolie worm we saw is right. Black on each end and a whole lot of brown in the middle. Winter will start off bad then have a long nice break of good weather only to end up ...Read More »
Rex and Shirley Halcomb went Friday evening and put Christmas flowers on our families graves at Highlonesome. Thanksgiving Day Rex and Shirley and Junior and Betty had dinner at David Halcomb's. Chris and Christy Lakey hosted a Thanksgiving dinner at ...Read More »
Gobble, gobble, hope everyone is full of turkey. Friday James and I visited Bob and Lou Rains and Gordan and Zelda Jones. Joel Blevins stopped by and visited and Russ Jones stopped by. Russ, Bub Payne, Brady Herd and Colton ...Read More »
Attendance at Mt. Olive (Highlonesome) was up Sunday. They had good services. The young people sang. We opened service with prayer reading from I John 3:1-3 and prayer requests were taken. Pastor Jerry Loveland preached on faith. Tina Swearengin and ...Read More »
Saturday evening several gathered at John and Connie Siler's to see their granddaughter, Kaylee Evans' 10 point buck. It was a very nice one. Her great-grandparents, Junior and Betty Halcomb and Kaylee's uncle, David Halcomb, and her great-uncle, Rex Halcomb ...Read More »
Sunday was a very nice day for the hayride hosted by the Garrison Church. They did the roadway trash pickup, hayride and weiner roast. We had a large crowd. We were very thankful for the great weather as it is ...Read More »
It sure would be nice to have a nice long fall, but the way the temps are it maybe short lived. The geese are traveling day and night. We picked up some walnuts and hope to finish them in the ...Read More »
Saturday we had a wonderful rain. My nephew, David Halcomb, gave me green beans. I canned a cooker and we have plenty to eat. He sure had a good garden. I think he got his green thumb from me. Yeah, ...Read More »
Friday evening we attended the Chadwick Carnival. Several children from Garrison Baptist Church attend school there and were attendants in the carnival. We visited with a lot of folks. Janie Morrison and her niece, Phyllis Bloomer, attended. I chatted a ...Read More »


text:
Havahart® 1.25 Deer and Rabbit Repellent Pellets (5620)
- Sub Brand: DeFence
- Animal Type: Deer & Rabbit, Deer
- Product Type: Animal Repellent
- Product Form: Granules
- Product Size: 1.25 lb.
- Duration: 90 day
- Concentration: 4.63 percent
- Active Ingredient: Putrescent Whole Egg Solids
- Number in Package: 1 pk
- Ready to Use: Yes
- Treatment Coverage Area: 625 sq. ft.
- OMRI listed for organic gardening
- Prevents deer and rabbit damage
- Protects garden, ornamentals and landscape up to 3 months
- Works year-round
- Fast acting- repels on contact Active ingredient: putrescent whole egg solids 4.63%
*Items shipped from our warehouse to stores in Alaska and Hawaii will receive a 70% shipping discount.
30-Day Return Guarantee
We want you to be fully satisfied with every item that you purchase from www.acehardware.com. If you are not satisfied with an item that you have purchased (excluding Gift Cards and gas-powered outdoor equipment*), you may return the item up to thirty days after the original purchase date to receive a store credit (less shipping, handling and other charges). We recommend that you make returns of online purchases to an Ace retail store near you which will issue your store credit. If you do not have an Ace retail store near you, please contact a Customer Service Specialist by calling 1-866-290-5334 for complete return instructions. Please refer to our Return Instructions for more information about returning a purchase.
The item must be returned in good condition, in original boxes (whenever possible), and with all paperwork, parts and accessories to insure full credit. After 30 days, please contact the manufacturer directly.
*Gas-Powered Outdoor Equipment cannot be returned once gas or oil has been put in it, even if the Equipment has been drained of these materials.


text:
There was a time when board games were wholesome family fun: mum, dad and children around the table, squabbling over whose turn it is and who owes what to whom. But a new game launched in the UK last week has a more sinister aim: make the most cash from dealing drugs without getting caught.
The game, called the Chaps from Amsterdam, is like the evil love child of Monopoly and TV's The Wire. It has enraged anti-drugs campaigners who say it both glamorises and trivialises the trade.
Players become dealers who vie to buy S.P.A.C.E (speed, puff, acid, coke and ecstasy) in Amsterdam for the lowest price and sell it on for the greatest profit.
Instead of Monopoly's Chance cards, there are Police cards, whose forfeits include: "Your bent copper loses the evidence; pay protection £150,000", and, "Your courier is busted for ecstasy; lose all your Es". Other cards detail the actions of informers and the courts.
The game is on sale online directly from its manufacturers and from eBay for £39.99. It was devised by a company called Hard Impact Board Games, who are careful to protect their anonymity. Describing themselves as "a collective of individuals from London aged 30-50", they claim not to have first-hand knowledge of the Dutch drugs scene, although one of the game's inventors is understood to have spent time in prison.
On their website they write: "The Chaps from Amsterdam has been formulated through years of hard work, in bedrooms, front rooms, pubs, clubs, raves, festivals, police cells and prison cells. Trust nobody and never be too flash with your cash as, like in real life, the police, courts and informers are waiting for you around each and every corner!"
Peter Stoker, director of the National Drug Prevention Alliance, said yesterday: "This so-called 'game' is a deeply cynical attempt to make money from a dirty business. They cite the usual get-out clauses about this game just being for 'educational, recreational and historical purposes' and they claim 'no intention to incite the use of controlled or illegal substances'. In fact it's self-evident that it's for money-making purposes, and, if it incites drug use, who are they to care? It's the rest of us who will have to deal with the damage they cause."
Mr Stoker continued: "They give the impression that drug use is a subject you can have fun with – but would you buy a board game about other illegal actions like robbery, or domestic violence, or rape? This game is just as damaging in its own way, and it belongs in the wheelie bin, along with its makers."
Martin Barnes, the chief executive of the charity DrugScope, said: "Games like this certainly don't help people to make informed decisions about drugs, and to some extent they trivialise the significant harms caused by the illegal drug trade."
Hard Impact Board Games denied this: "It's a game and it shows quickly the possible consequences of engaging in this type of activity, such as time in prison or rehab. The game also has some squares that will take you out of the game completely to show the ultimate consequence of drug taking and drug dealing."
Asked whether one of the game's inventors had spent time in prison and whether experience of drugs-related crime had been incorporated in the game, the spokesman declined to comment, saying: "It's just a concept of how things might be in that scenario. At the end of the day, it's a game."Reuse content


text:
Hello. Sign In
Please enter a valid city, state or zip code
18 reviews | 3.7 out of 5
I had previously purchased hanging solar outdoor lanterns from Sam's club and was hoping these were similar. But they are of inferior quality. They are plastic, smaller, and the light is not very bright. Instead of glass windows it's white plastic. We are very disappointed.
Disapointing, low light level, very light plastic so they keep blowing away in low wind. Not a good buy!
There's nothing quite like driving into the alley at night, coming home from a long day away, and being greeted by the twinkling of these lights along the gardens that edge my property boundaries and driveway! Trouble is, often they don't work. Many times a tap on the unit will cause the lights to go on. Other times it's of no use. I've probably bought 24 of these in order to have 8 that work. Make that work(ed), because 2 have just quit and nothing seems to jump start them. I love them, felt they were a good value, just wish they would ALL work ALL the time. I guess I go with the oft-amended line, "If we can put a man on the moon, why can't we have simple solar lights that work?"
Does not hold a charge for the time stated. Amber bulbs make almost useless for lighting
The lights are ok when they work. I tried to buy more but couldn't find any on the web site.
I've had this product a few weeks now and they look cool, but don't work as good as they look.
It takes awhile to get them charged and unless you can get them in direct sunlight for most of the day they won't make it through the night lit. They also aren't too bright, so you can't really rely on them for light, but they look cool if you just want to put them somewhere for looks.
The lights look just like a low fire glow in a lantern. Not much light given off, but they sure are pretty for what I want them for. They don't seem to hold the charge very well, but haven't had sunlight all day yet, so maybe will do better in the summer. The hangers are made sturdy and the lanterns are a good size. Very nice for the price!!
I think these lights are very nice outside my gazebo, however, one of the lights is more orange than the other (brighter). The poles are very strong and stand well in their holders.
These coach lights are a nice touch to the walk and the house.
Nice looking, work well. Hangers are a little flimsy
I love this set. It's really cute and cozy, yet handy. I have these right next to my door. If for some reason I get home and my outdoor light isn't on, I take a lantern up with me so I can see the keyhole in my door. I put these in the ground in the fall. I can't wait to use them in the summertime when I'm outdoors at night. They work great for kids as well when they're out playing in the yard. I'm really happy with these lights.
The only reason I did not give 5 stars is becuase I
had to buy a battery to replace one that did not work. They look beautiful!
The Lanterns are nice. They are of very nice quality BUT the lights inside are orange. They don't put off light enough to see by and they are a great disappointment. I thought they would show off the front of my house but they don't do anything, Looking for some bulbs or whatever to replace the insides. Bad decision on my part, I gave my other solar lights away.
These little lantern like solar lights are great. They glow an orange light at night to make you think they are really a lantern. We put one out at the cemetary on my Father-in-Laws grave and it looks wonderful. Would recomend these to everyone, and the price was very reasonable and the delivery time was excellent. Thumbs up on this all the way around!!!!
Could not wait afte purchasing the lights, to get the in our garden. After charging for a fewhours, they were glowing that night. Very pleased with this product.
These lanterns look great out by my gazebo. They are very sturdy and looks like they won't rust. Very happy with my purchase.
totally plastic,they look nicer in the picture,because they're not metal they dont go as well with other lights I have


text:
Porta Hotel Antigua
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Spa
- Suites
- Airport Transportation
- Drycleaning onsite
- Meeting rooms/conference facilities
- Banquet room
Photos of Porta Hotel Antigua
Travelers also recommend:
- Spa
- Heated pool
- Swimming pool
- Restaurant
- Outdoor pool
- Kids pool
- Fitness/Health center
- Bar/lounge
- Breakfast Available
- High-speed internet in room (surcharge)
- Wireless internet connection in public areas
- High-speed internet in room
- Wireless internet connection in room (surcharge)
- Reduced mobility rooms
- Non-smoking rooms
- Non-smoking hotel
- Wheelchair accessibility
- Free parking
- Pets allowed
- Family rooms
- Suites
- Drycleaning onsite
- Meeting rooms/conference facilities
- Airport Transportation
- Conference facilities
- Concierge desk
- Banquet room
- Babysitting service
- Room service
- Multilingual staff
- Business Services
- Meal plan
- Laundry
- Kids activities or Babysitting
TravelPod Member ReviewsPorta Hotel Antigua Antigua Guatemala
What a gloriously peaceful spot could stay here for a week
This review is the subjective opinion of a TravelPod member and not of TravelPod.com.
TripAdvisor Reviews Porta Hotel Antigua Antigua Guatemala
Travel Blogs from Antigua Guatemala
Manelleadventures venture back to El Salvador
... of a hike it was a slow incline which was great and we stopped to take pictures at varies view points which was lovely! Arriving at the top of the volcano was so cool looking down inside of the crater and seeing lava was just amazing and unbelievable. We took some pictures and then I sat their and took in the view of this splendid volcano. Preparing to go down was fun we ran part of the way down which was scary but adrenaline pumping kind ...
Suchitoto and the journey there
... was attending to other urgent business and would be back at 12; when I am leaving. Oh well, sister Peggy told me there had been one death and an invalid lady needed to be carried somewhere.I thanks her for her time. Many of the museums are run by the church in these parts. Next, I returned to Case de la Abuela and tried the chocolate artesana, a hot chocolate drink containing pure chocolate (with no preservatives). Lastly, I went to the internet cafe, skyped my ma and wrote this.
Food, waterfalls, horse rides & civil war history
... in the valley and Izalco volcano right next door, all in a beautiful panorama.
Once we were back down the volcano, a lovely group of locals asked us if we wanted to go out with them to a local bar, so we agreed they could pick us up later at our hostel. We got stood up though, as they ...
Beautiful Black Sand beneath my toes
... giant lake, on another side you could see several pueblitos sprinkled about and then finally, on the last side glittering in the sun was the ocean. Unreal, so incredibly beautiful. We just sat at the top for a long time taking it all in before we decided to climb down. At the base right as we were about to get in the car we realized that when we were taking pictures at the top had left the car keys on top of the rock....I wanted to cry, but of ...


text:
Sycamore Partners buys The Limited for $26.75 million
The apparel chain filed for bankruptcy in January and closed its e-commerce site and stores.
Sycamore Partners buys The Limited for $26.75 million
The apparel chain filed for bankruptcy in January and closed its e-commerce site and stores.
Nordstrom eclipses $3 billion in online sales in 2016
Online sales now account for more than 22% of Nordstrom's total sales.
Sign up for IRNewsLink and get daily reports delivery to your inbox. Sign Up Now
Sponsored by 2013 Social Media 300
Liberty Media knows its way around online retail deal integration.
Liberty Media Corp, which owns and operates QVC.com, No. 11 in the Internet Retailer Top 500 Guide, has a good eye for acquisitions and an even keener sense for making the operation mesh once the deal is done. In the last few years, I've watched Liberty Media take what might seem like a series of random acquisitions, including ProFlowers.com, BuyCostumes.com, RedEnvelope.com, Bodybuilding.com and Backcountry.com, and turn the collective buying spree into a highly integrated business unit that's delivering pretty good top-line growth. In the first quarter ended March 31, sales for Liberty Media's acquired e-commerce companies increased 10.1% year-over-year to $268.0 million from $243.5 million. In 2009, those same integrated acquisitions grew sales 20% to $931.0 million from $776.0 million in the prior year. Sometimes when smaller web retailing companies are gobbled up by a bigger entity, the two sides don't mesh and senior executives from the acquired company – those entrepreneurs who knew the ins and outs of selling to their niche – bide their time and when their contract is up immediately take off for the next venture. But it's been four years since Liberty Media acquired BuyCostumes.com and founder and CEO Jalem Getz is still on the job using the big marketing base and deep resources of Liberty Media to grow his unit's merchandising niche in costumes and costume accessories. Finding a way to make acquisitions work and get different personalities and cultures to click is a pretty important job in today's consolidating e-commerce market. The deals are only going to intensify and the bigger players will only get bigger – witness Amazon.com's $900 million takeover of Zappos.com last year. Liberty Media has learned what it takes to make a key acquisition, make the parts fit into a bigger organization and grow the business. That's a lesson most other web retailers of any size have to learn as well.
Certain website features, such as customer reviews, have relatively little impact on driving traffic, but ...
Posted 08/06/15
When you think of consumer-facing e-commerce, you probably think of Amazon.com Inc., the dominant player ...
Posted 02/11/14
In the forefront of B2B e-commerce for many years, W.W. Grainger knows what it takes ...
Posted 02/04/14
Minimize clutter and maximize clarity, advices Jeffrey Vocell of testing and optimization firm SiteSpect.
Posted 10/07/13
The Supreme Court is surely going to find this question right back on their doorstep next year."


text:
Makes 8 wands
Let's get dipping!
4 ounces vanilla candy coating
or almond bark
Powdered food coloring
(optional: see note)
8 large pretzel rods
Assorted colored sugars, sprinkles,
and sugar candy decorations
Melt white candy coating according to package directions. Add powdered food coloring if desired. Stir to blend.
Dip or drizzle pretzel rods with candy coating. Place coated pretzel rods on a foil-lined sheet pan that has been sprayed lightly with nonstick cooking spray. Decorate with sugars, sprinkles, or candies while candy coating is still warm.
Place sheet pan in freezer for 10 minutes to harden candy coating. Remove and eat immediately or store in an airtight container for several days.
They're magical!
Liquid and paste food colorings cause melted candy coating and chocolate to "seize." This means when it is added to the melted ingredient, it makes it clump together and become lumpy and stiff. That's not good! Powdered food coloring may be found in craft or cake decorating stores and is the only type of food coloring you should use when working with chocolate and candy coating.
Pink Princess Cookbook
by Barbara Beery
Gibbs Smith
Hardcover with concealed spiral binding; $14.95
Recipe reprinted by permission.
This page created October 2006
The FoodWine
Main Page
Kate's Global Kitchen
Kate's Books
Cookbook Profiles
Global Destinations
Holiday & Party Recipes
I Love Desserts
On Wine
Caffeine and You
Cooking with Kids
New Green Basics
Conversions, Charts
& Substitutions
About FoodWine
Contact Info
Privacy Statement
Anatolia: Turkish Recipes
Bar Tartine
The Beer Bible
Beetlebung Farm Cookbook
Bird in Hand (Chicken)
Bitter: Flavor
Bob's Joke Burgers
Cooking Light
Darjeeling Tea
Dinner at Home
Everyday Baker
Fast Food (Andrew Weil)
Flavor Flours
Food 52 Genius
The Food Lab
Garden Recipes
Grandmas' Recipes
Hartwood: Yucatán
Humane Butchering
Heritage Southern Recipes
Jemima Code African Recipes
Kitchn Cookbook
Liquid Intelligence
Near & Far World Recipes
NOPI Restaurant Cookbook
Oxford Companion to Wine
Phoenix Claws: Chinese
Picnic: Recipes
Soda Politics
Southern Recipes
The Third Plate
V Is for Vegetables
Vegetarian India
What Katie Ate
The Whole 30
Whole Food Kitchen
Yucatán Recipes
Zahav Israeli Cooking
Copyright © 1994-2016,
Forkmedia LLC


text:
FIND YOUR HOME IN Moran
Moran Real Estate
Moran is located in Kansas. Moran, Kansas has a population of 432. Moran is less family-centric than the surrounding county with 25.22% of the households containing married families with children. The county average for households married with children is 28.39%.
The median household income in Moran, Kansas is $38,333. The median household income for the surrounding county is $39,714 compared to the national median of $53,046. The median age of people living in Moran is 47.6 years.
Moran Weather
The average high temperature in July is 92 degrees, with an average low temperature in January of 22.5 degrees. The average rainfall is approximately 39.7 inches per year, with 13 inches of snow per year.
Moran Map
Moran real estate offices
Real estate near Moran
Nearby Cities
Moran Zip Codes


text:
Out Now
First Contact by me and Bryan Coyle features in the pages of FutureQuake's 2016 Winter Edition, available from the FutureQuake Press site, priced £6.50 (+ shipping) or digitally from Comixology, priced £1.99.
Strontium Dog: Old Laing Zyne by me and Scott Twells is a free-to-air daily strip produced for Zarjaz.
Judge Dredd: Frostee The Snow-bot by me and Bolt-01 is a free-to-air daily newspaper strip.
Anderson, Psi Division: Clear & Present Danger by me, Dunk Nimmo and Bolt-01 is a free-to-air daily newspaper style strip.
The 13th Stone is a self contained digital one-shot by myself and Bryan Coyle, available from Gumroad, Sellfy and Payhip where you can pay whatever you want!
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales License.


text:
Results 1 to 1 of 1
- 01-13-2013, 04:44 PM Thread Author #1
- Posts
- 52 Posts
- Mentioned
- 0 Post(s)
- Tagged
- 0 Thread(s)
homework to do list
hey guys, i wanna know if anyone knows a good to do list or task list or whatever you wanna call it, for keeping in track of doing my homework. i want an app thats simple and quick, and have a nice Holo themed UI(optional), and also just work well in jotting dow my homework assingments.
Similar Threads
Shared to-do list (without subscription fees)By WarER4X in forum Android AppsReplies: 11Last Post: 04-05-2014, 02:58 AM
To Do list that syncs with PC?By Phoebe_n in forum Android AppsReplies: 9Last Post: 11-21-2010, 11:46 PM
a good TO DO LIST?By ricbon in forum Android AppsReplies: 25Last Post: 11-04-2010, 02:20 PM
To do list before a factory reset?By Wanda in forum Verizon Droid IncredibleReplies: 9Last Post: 10-07-2010, 10:51 AM
New Droid Incredible: best apps for battery, brightness, and To Do listBy mvershel in forum Verizon Droid IncredibleReplies: 13Last Post: 08-20-2010, 04:39 PM


text:
Hash House A Go Go Send to Phone
OpenBreakfast, Lunch & Dinner daily, Brunch Sat.-Sun.
- Parking lot
- Dress code: Casual
- Full bar
- Kid-friendly
- Outdoor dining
- Reservations suggested
* Click here for rating key
Text data on Hash House A Go Go to your phone, or a friend's cell phone.
Find out which restaurants and chefs top the list of GAYOT's 2016 Annual Restaurant Awards.


text:
Turns WordPress instalation into powerful pizzeria site backend with ability to add pizzas, beverages and pasta.
Clean and useful pizza overview for administrators
Frontend pizza filtering for easy choose
Handy extended description for your pizzas
Easy to use settins page
Simple and Quick admin menu
Edit your pizza's fast right from the overview
Ability to add ingredient image
Extended dashboard Right now widget
Requires: 3.4 or higher
Compatible up to: 3.5.2
Last Updated: 4 years ago
Active Installs: Less than 10
Got something to say? Need help?


text:
Jungbunzlauer's product groups
Based on years of experience and acquired knowledge, Jungbunzlauer offers a broad spectrum of biodegradable key ingredients of natural origin to a diversified range of industries worldwide.
Jungbunzlauer's main markets
Jungbunzlauer's know-how and experience in ingredients guarantees innovative solutions for a broad range of applications. In order to respond to changing consumer preferences.
Contact by region
Products smartfinder
Jungbunzlauer offers a broad variety of high quality products. Get the best matches for your application:
Latest news
ExCel, London, 04 April, 2017 - 06 April, 2017
Jungbunzlauer, a leading producer of biodegradable ingredients of natural origin, is again exhibiting at this year's in-cosmetics. The company tr...Read more
14 February, 2017
The demand for certified Non-GMO food is steadily increasing. More and more consumers seek reliability about the origin of their daily food and the...Read more
06 February, 2017
As a result of product and applications developments, Jungbunzlauer introduces innovative solutions to the industry of dairy and meat alternatives....Read more
Upcoming trade fairs
Jungbunzlauer at the in-cosmetics 2017
London, 04 April, 2017 - 06 April, 2017 Read more
Booth: B30
Jungbunzlauer at the PCHi 2017 in China
Guangzhou, 21 February, 2017 - 23 February, 2017 Read more
Booth: 3J48
Jungbunzlauer at the Hi Europe
Frankfurt, 29 November, 2016 - 01 December, 2016 Read more
Booth: F37 / hall 3


text:
Don't forget, you have $50.00 on us
Use code GIFT50 for $50 off any first purchase over $400.
Added to Shopping Bag
Kabana Black White Ring 0.06cts Diamond 14k White Gold Onyx Mop
Listed by Luxebuster
Size: N/A
Sale: $1,031.05
Returnable for Site Credit.
You can request a return for Tradesy Site Credit within 4 days of delivery. FREE return shipping.
If your item was misrepresented in any way, you can file a misrepresentation claim. Tradesy will investigate and you'll be eligible for a Full Refund.
Shopping Bag :
Reserved for -:--
Kabana Black White Ring 0.06cts Diamond 14k White Gold Onyx Mop
Shipping Included
Ships Next Day. Order today. Ships tomorrow.
Pay with Credit Card, PayPal, or Affirm.
2 people saved this item
This item has original tags and shows no visible signs of wear.
Kabana Black White Ring 0.06cts Diamond 14k White Gold Onyx MOP Sz 7 NWOT $2435
Ad # 30779

Item Description:
Kabana B&W Ring 0.06cts Diamond 14k White Gold Onyx Mother of Pearl Sz 7
***New Without Tag***
Retail Price: $2,435
Designer: Kabana
Model #: WRIF488OXMW
Item: Finger Ring
Clasp: N/A
Description: Alternating black onyx and white mother of pearl, separated by diamond clusters, set in white gold
Composition: 14k White Gold, Diamonds (0.06 TCW, SI1 quality and G-H color), Mother of Pearl, Onyx
Total Weight, Grams: 7.7
Measurements: Size 7, 9mm widest, 4mm narrowest, 5mm dome
Markings: Kabana, 14k
Condition: The piece is new, with the retail tag(s) removed.
This will appropriately packed to ensure safe arrival.

To get a real-time shipping quote to your destination, enter your country and zip / postal code information into the shipping calculator and click Get Rate.
USPS Priority Mail
Put your auctions on autopilot with Kyozou eBay Auction Tools
Wed 3/1/17 - Sat 3/4/17 (3-6 days)
Feel free to return this item for any reason and get Tradesy Credit. Use your credit to buy something that makes you happy. Just a heads-up, purchases outside the U.S. are Final Sale.
Submit a return request within 4 days of delivery
Pack and ship the item using our free return shipping label
Skip the return request and bring the item to your nearest for instant Tradesy Credit.
Have a similar item? Sell Yours
Authenticity Promise
Tradesy only allows the sale of authentic designer goods. If you believe your purchase is a replica, send it to our team for a free evaluation. You'll get a full refund if anything's amiss.*Learn More
Our Guarantee
Get what you ordered, or your money back. We'll give you a full refund if the condition, style, or authenticity of an item are misrepresented in any way.*Learn More
Real People, Here to Help
Need help? Have questions? Our team in sunny Southern California is available by phone, email, and chat.Help & Support


text:
This servicemen featured in this drum-battle between the III Marine Expeditionary Force band and the Republic of Korea look like they are having a great time with a little friendly competition.
The video, uploaded to YouTube earlier this week pits the United States Marines against the ROK's best in a parking lot drum battle.
Even though they declared a tie, it's safe to say that the Americans won this round.


text:
- Players
- 1
- Genre
- Action
- Release Date
Wii U eShop
- 17th Apr 2014, $6.00 (USA)
- 3rd Jul 2014, £4.00 (UK/EU)
- Series
- Flowerworks
- Official Site
- nocturnal-central.com
About This Game
Follie has mysteriously crashed on an unknown planet. Solve the mystery and help Follie escape!
During a routine space trip, Follie's ship mysteriously malfunctions and crash lands on Elilia, losing all her precious Flowerwork seeds. Before long, her very sanity is threatened by drab surroundings and uncooperative gnomes.
Help Follie find her seeds, use Flowerworks to transform her surroundings and show those gnomes who is boss on the way to unlocking the secret of Elilia.


text:
As alumni of Clarke University, you likely felt Clarke was more than a school to you. It was a place that felt more like home. A place where you formed friendships, celebrated traditions, and made memories that you likely still cherish.
Today, through the Clarke Lives Endearment Fund, you can impact a whole new generation of students eager to make a difference in the world. Students who will gain so much from the valuable life lessons, deep traditions, and exceptional education they'll experience. Clarke lives in you and because of you, it will live on for generations to come.
Start your living legacy today. Call 888-225-2753.


text:
By Jefferson Graham, USA TODAY
SAN JOSE, Calif. Google CEO Eric Schmidt has a message for Google users: Your searches are safe.
AOL has been in hot water this week for inadvertently releasing customer searches for a research project. But Schmidt, speaking at an industry conference here, says Googlers have nothing to worry about.
KEVIN MANEY: AOL's data sketch sometimes scary picture of personalities searching Net
"Our No. 1 priority with our users is trust," Schmidt told some 2,000 attendees at the Search Engine Strategies conference. "It won't happen. We have systems in place that won't allow anything like that to happen."
Safa Rashtchy, an analyst at equity firm Piper Jaffray, takes Schmidt at his word. "Google is one of the most protective companies on the planet," he says. "They have a culture of secrecy and would never let anything like this happen."
This week Schmidt has been deep into what he describes as "deal mode," cutting landmark alliances with Viacom's MTV and News Corp.'s Fox Interactive Group to greatly expand Google's advertising network.
"These are huge deals," Rashtchy says. "Video is moving to the Web, and Google has figured out a way to make money from it."
Schmidt says the goal was to bring Google's red-hot advertising network beyond text ads into video. The MTV deal lets specific website and blog owners take clips from MTV and Nickelodeon TV shows, post them and get a majority cut of the advertising revenue that Google will sell.
"We see this as a whole new category, an undermonetized opportunity to reach an audience we're not reaching today," says Schmidt.
He's particularly optimistic about the growth of social networks, like Fox's MySpace, which has come from nowhere to become the sixth most-visited website, according to measurement firm ComScore Media Metrix. Additionally, Internet users spend more time at MySpace than at any other website, says ComScore.
"Social networking isn't a fad, but a real phenomenon," says Schmidt. "It's not something that will go away."
The Google/MySpace deal, valued at $900 million, brings Google's ad network to MySpace and other Fox properties.
Schmidt said he makes tons of searches daily on Google and uses it as a truth squad. He told of a politician who came to visit him who said there were more outhouses in the world than users of the TiVo digital video recorder.
After the politician left, Schmidt looked it up. "We live in a world where people make all sorts of claims, and I always wonder if they're true," he said.
Turned out the information was old — there are more TiVo users, he says. "Having the ability to search and get the answer is a nice way to live your life," he says.


text:
Greetings Guest!!Dismiss Notice
In order to combat SPAM on the forums, all users are required to have a minimum of 2 posts before they can submit links in any post or thread.
[Price Check] garg ring with hci ep ssi skill stam inc
Discussion in 'UO Trader's Hall' started by 4runnersport, Feb 21, 2015.


text:
- Parents.com
- Fun
- Arts & Crafts
- Photography
Now that digital cameras are affordable for all, it's a great time to introduce your child to photography and explore how he sees things. Learn the basics of photography like framing up the shot and focus, then try special effects to create a unique and artistic image.
12 Best Photo Apps for Moms
With all of the amazing photo apps available, there's simply no excuse not to be snapping away at your kid multiple times a day. The only hitch is deciding which apps are the best, and easiest, to use. Download any of these 12 mom-approved photo apps for your iPhone, iPad, or Android device to instantly feel like a professional photographer.
Make Your Family Photos Fun
We rounded up some super-simple photo ideas to help you pump up your pictures and put that family album back on your to-share list.
Creative Ways to Document Baby's First Year
A cute, personal memento of the first 12 months of parenthood is not only fun to make, it's also something you and your family will treasure for the rest of your lives.
10 Best Photo Gifts
We scoured the Internet to find affordable, fun photo gift options that anyone would appreciate receiving.
Simple Ways to Organize Your Family Photos
Are you suffering from digital photo overload? Get your photo collection under control with our guide to organizing, printing, and creating fun keepsakes from all those great shots.
Get Your Kid Noticed in Photo Contests
Use these tips to help your kid's photo rack up the most votes in one of our photo contests.
How to Dress for Family Photos
Family photos live on for decades, so you want to make sure your crew looks dressed to impressed. Here are some tips to get your group camera-ready.
How to Protect Your Kid's Photos Online
It's possible to protect photos of your child online when you follow these simple guidelines.
Cool Photo Crafts
10 simple ways to give your fave photos a new life -- on and off the computer.
Should You Hire a Birth Photographer?
One more thing to add to your hospital checklist? A birth photographer! Find out if hiring one is the right choice for your baby's big day.
Fun Crafts with Your Digital Camera
Use your digital camera to create cool crafts -- you won't believe how easy they are to make!
Readers' Best Family Vacation Photos
School season's here, but the weather's still ripe for outdoor activities. Gather the kids and try these ideas inspired by the summer photos Parents readers submitted in our first-ever Family Vacation Photo Contest.


text:
4 works Add another?
Most Editions | First Published | Most Recent
Reflections on the seven days of the week13 editions - first published in 1770
Essays on various subjects3 editions - first published in 1772 Read
The works of the late Mrs. Catharine Talbot2 editions - first published in 1780
Essays on various subjects in prose and verse1 edition - first published in 1773
Links (outside Open Library)
No links yet. Add one?
History Created April 1, 2008 · 3 revisions
|June 7, 2012
|Edited by VacuumBot
|Removed period from death date
|September 6, 2008
|Edited by RenameBot
|fix author name
|April 1, 2008
|Created by an anonymous user
|initial import


text:
Plenty of upsets on first day of NEC Wheelchair Tennis Masters tournament
MISSION VIEJO – There were plenty of upsets in first-round play of the 2013 NEC Wheelchair Tennis Masters tournament at the Marguerite Tennis Center Tuesday.
The biggest occurred in Pool 2 of the Men's draw when the world's No. 2 player, Stephane Houdet (France), fell to Joachim Gerard of Belgium, 6-3, 2-6, 6-3.
In that same draw, Maikel Scheffers of The Netherlands – the world's No. 3-ranked player – fell to 2008 Olympic gold medalist Michael Jeremiasz (France), 4-6, 6-4, 7-5.
And in women's competition, No. 1 seed and 2013 French Open champion Sabine Ellerbrock of Germany was defeated by Jordanne Whiley of Great Britain, 2-6, 6-1, 6-1. Whiley, 21, was Great Britain's youngest national wheelchair champion at age 14, and won a bronze medal in doubles at the 2012 Summer Parlympics.
In Pool 1 action, the world's No. 1 men's player, Shingo Kunieda of Japan, easily defeated No. 5-ranked Gustavo Fernandez of Argentina, 6-0, 6-1, and No. 4-ranked Gordon Reid of Great Britain beat No. 8 Nicolas Peifer of France in straight sets, 6-1, 6-2.
In the women's draw, No. 2 seed and nine-time Grand Slam champion Jiske Griffoen of the Netherlands rolled past Kgothatso Montjane (South Africa), 6-1, 6-1. Other winners in the Women's tournament included Japan's Yui Kamjii, the youngest player in the tournament at age 19, defeating Sharon Walraven in straight sets, 6-2, 6-0, and former French Open doubles champion Marjolein Buis of The Netherlands defeating British national champion Lucy Shuker, 6-4, 1-6, 6-4.
In Quads competition, the No. 1-ranked player in the world, David Wagner of Chula Vista, defeated No. 3 seed Andy Lapthorne (Great Britain), 7-5, 6-0, and No. 2 seed Lucas Sithole (South Africa) beat No. 4 Nicholas Taylor of the United States, 6-0, 6-4.
Action continues today from 10 a.m.-2 p.m. in Men's, Women's and Quad competition.
The tournament runs through Nov. 11 with day (10 a.m.-2 p.m) and night sessions (6-10 p.m.). The ITF Wheelchair Doubles Masters tournaments in Men's, Women's and Quad divisions will begin on Thursday.
For more information, visit itfwheelchairmasters.com. The tournament will be streamed live online at cityofmissionviejo.org/mvtv.
Contact the writer: email@example.com or 949-454-7309
Most Popular
- Suspect in Whittier cop killing, East L.A. slaying was AB 109 probationer
- 2 juveniles arrested after fight with off-duty police officer in Anaheim, a gun was fired
- Video shows Harrison Ford wrongly flying over plane at John Wayne Airport
- Oram: Incredulous, overconfident, tilting at windmills: How Lakers' Jim Buss did himself
- 2 new memos allow President Trump to set broad deportation rules for undocumented immigra
User Agreement
Comments are encouraged, but you must follow our Privacy Policy Policy & Terms of Use
Keep it civil and stay on topic. No profanity, vulgarity, racial slurs or personal attacks. People who harass others or joke about tragedies will be blocked. By posting your comment, you agree to allow Orange County Register Communications, Inc. the right to republish your name and comment in additional Register publications without any notification or payment.


text:
Hi, being a noobie on here I am hoping this is in the correct section
Has anyone in here fallen pregnant while breastfeeding and on the mini pill?
My daughter is nearly 11 months and breastfeeding regularly (up to 6 times a day but sleeping 12-13 hours at night), I take the pill every morning, give or take an hour or two. My periods have not returned (they didn't with my son until I went off the pill when he was 14 months old, got pregnant after my first period which was a week after stopping the pill)
The last week I have been very queasy, food smells while cooking make me not feel like eating what I've cooked and feeling generally tired and headachy.
I have POAS which come up negative. I have an app for the Dr on Wednesday so will get a blood test.
Please tell me it is highly unlikely- hubby and I have decided that we won't be trying for anymore kids (but a whoopsie baby would be very much loved anyway)
(my sister fell pregnant while BF and on the pill but wasn't regular with the times she took it, he girls are 12 months apart )


text:
Lemon Broccoli
|1⁄2 Cup (8 tbs), slivered
|1 Tablespoon
|Broccoli spears
|20 Ounce, frozen
|Cream cheese
|8 Ounce
|1⁄3 Cup (5.33 tbs)
|Grated lemon juice
|1 Teaspoon, grated
|1⁄2 Teaspoon, ground
|1⁄4 Teaspoon
Combine almonds and butter in small glass bowl.
Microwave on medium for 3 minutes.
Stir and continue cooking on medium 2 to 3 minutes or until almonds are light brown; set aside.
Place frozen broccoli in 2-quart glass casserole.
Cover with glass lid or plastic wrap.
Microwave on high for 4 to 5 minutes or until broccoli is tender.
Cover, and continue cooking on high for 6 to 7 minutes or until tender-crisp.
Let stand, covered.
Place cream cheese in 2-cup glass measure, Microwave on low for about 4 minutes or until softened.
Cream until smooth.
Stir in remaining ingredients.
Microwave on medium for 3 to 4 minutes or until hot.
Place broccoli spears on serving platter and pour sauce over.
Sprinkle with almonds and serve.
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 1536 Calories from Fat 1125
% Daily Value*
Total Fat 130 g199.6%
Saturated Fat 55.8 g278.8%
Trans Fat 0 g
Cholesterol 289.2 mg96.4%
Sodium 1432.4 mg59.7%
Total Carbohydrates 67 g22.4%
Dietary Fiber 23.5 g94.1%
Sugars 23.8 g
Protein 47 g94.5%
Vitamin A 137.1% Vitamin C 847%
Calcium 76.7% Iron 42.8%
*Based on a 2000 Calorie diet


text:
Don't think there should be a gardenchat discount... maybe a percent of our
purchases should go into the hort fund instead.
Of course, I am assuming you will be charging comparable pricing to other
garden nurseries/centers.
> If there's enough interest I can definitely get some -- I have a source.
> The question is, how much should the gardenchat discount be? :)
> Chris
To sign-off this list, send email to email@example.com with the
message text UNSUBSCRIBE GARDENCHAT


text:
We initiated the entrepreneurial journey by starting an IEDC under the mentorship of TTBI, Trivandrum. As a result of this great beginning, many of our students have come up with new innovative ventures. Also we have filed number of patent applications through KSCSTE. Presently, we are one of the best performing IEDCs under DST. To realise the inspiration and enthusiasm of our student community into marketable products, it is essential to scale up the IEDC to the level of TBI.
- Creation of Technology-Based incubates on continuous basis
- Help to create value-added jobs and services
- Introduction of Entrepreneurial culture to the students and common people.
- Create effective networking for the development of technology based start-ups
- Develop internationally accepted technologies
- Promote students to come up with commercially viable curriculum projects
- Create student entrepreneurs
- Create awareness about Technology Incubation and Commercialization of R & D products and processes.
- Promote small and medium industries.
An overriding objective of the venture is to achieve a transformation of minds of the students from being job-seekers to being entrepreneurs themselves, utilising the theoretical and practical lessons they get in the college. The TBI will also be the beginning of a stronger industry-academia-consumer linkage where ideas can flow to and fro through the three stakeholder groups so that everyone benefits. The industry will benefit from technological developments initiated by the TBI as well as qualified students who have an innovative mind to join their workforce. Academia will stand to gain from its constant interaction with industry, and consumers will gain from the innovations that are rolled out through the association.


text:
(952) 593-1169
Alerus Financial, N.A.
26 years in business11100 Wayzata Blvd Ste 570
Minnetonka, MN 55305-5534
Fax Numbers
- (952) 593-1634
- (651) 203-8339
Additional Email Addresses
- email@example.com
Find a Location
BBB File Opened: 11/21/2008
Business Started: 01/01/1991
Business Started Locally: 01/01/1991
Business Incorporated: 01/20/1995 in ND
Type of EntityCorporation
Business Management
- Jan Fitzer, President
- Nancy Johnson, Controller
- Solveig Nelson, Executive Vice President
- Kim Onnen, Vice President
Contact Information
- Principal: Jan Fitzer, President
Business Category
- Mortgage Brokers
- Mortgage Lender
- Mortgage Bankers
Service AreaAFTON, MN, ALBERTVILLE, MN, ANDOVER, MN, ANOKA, MN, APPLE VALLEY, MN, ARDEN HILLS, MN, BAYPORT, MN, BELLE PLAINE, MN, BETHEL, MN, BIG LAKE, MN, BISCAY, MN, BLAINE, MN, BLMNG PRAIRIE, MN, BLOOMING PRAIRIE, MN, BLOOMINGTON, MN, BROOKLYN CENTER, MN, BROOKLYN CTR, MN, BROOKLYN PARK, MN, BUFFALO, MN, BURNS TOWNSHIP, MN, BURNS TWNSHP, MN, BURNSVILLE, MN, CAMBRIDGE, MN, CANNON FALLS, MN, CENTERVILLE, MN, CHAMPLIN, MN, CHANHASSEN, MN, CHASKA, MN, CHISAGO CITY, MN, CIRCLE PINES, MN, COLLEGEVILLE, MN, COLUMBIA HEIGHTS, MN, COLUMBIA HTS, MN, COLUMBUS, MN, COON RAPIDS, MN, CORCORAN, MN, COTTAGE GROVE, MN, CROWN COLLEGE, MN, CRYSTAL, MN, DELANO, MN, DELLWOOD, MN, EAGAN, MN, EAST BETHEL, MN, EDEN PRAIRIE, MN, EDEN VALLEY, MN, EDINA, MN, ELK RIVER, MN, ELKO NEW MARKET, MN, ELKO NEW MRKT, MN, ELKO, MN, EXCELSIOR, MN, FALCON HEIGHTS, MN, FALCON HGTS, MN, FARGO, ND, FARIBAULT, MN, FARMINGTON, MN, FOREST LAKE, MN, FRIDLEY, MN, GLENCOE, MN, GOLDEN VALLEY, MN, GRAND FORKS, ND, GREENWOOD, MN, HAM LAKE, MN,
Alternate Business Names
- Alerus Mortgage
- Residential Mortgage Group/Alerus Financial, N.A.
Licensing, Bonding or RegistrationThis business is in an industry that may require professional licensing, bonding or registration. BBB encourages you to check with the appropriate agency to be certain any requirements are currently being met.
According to the information provided by Alerus Financial, this company offers residential mortgage and escrow services and full-spectrum lending programs.
BBB Reason for Ratings
Licensing information is provided in the BBB Business Profiles to inform the public about industries that may require professional licensing, bonding, or registration. Better Business Bureau encourages you to check with the appropriate agency to be certain any requirements are currently being met.
Advertising Review
BBB promotes truth in advertising by contacting advertisers whose claims conflict with the BBB Code of Advertising. These claims come to our attention from our internal review of advertising, consumer complaints and competitor challenges. BBB asks advertisers to substantiate their claims, change ads to make offers more clear to consumers, and remove misleading or deceptive statements.
Government Actions
BBB reports on known significant government actions involving the business's marketplace conduct.
Out of Business
BBB reports on a company that is out of business for three years from the date the company closes its doors or ceases to do business.
Misuse of Better Business Bureau Name/Logo
BBB reports on unauthorized use of the Better Business Bureau's name and/or logo for as long as the business continues to use it in any advertising, or for one year after the business ceases any repeated unauthorized uses.
BBB reports on a business's bankruptcy as long as the business remains in bankruptcy.
Mail Returned
BBB reports when mail sent to the business was returned by the Postal Service.


text:
*This item is not regularly stocked or displayed in our Showroom, however it may be Special Ordered for you.
Item Availability is subject to Manufacturer Drop or Discontinuation & Price may vary according to fabric grade selected. Please stop in or click Request More Info for additional details.
The casual-contemporary style of this 3 pc. sectional will freshen up large living rooms and family rooms with a clean-cut look. Designed to be simplistic with a hint of modern fashion, this group seating features plush seat cushions with large accompanying toss pillows. Exposed tapered wood legs and arched track arms add trend-setting elements from contemporary design. The perfect sectional sofa for family room fun, this piece seats five or more guests and fits easily into corners.
The Gulliver Casual-Contemporary 3 pc. Sectional from Rotmans. We proudly serve the Worcester, Boston, MA, Providence, RI, and New England area. Product availability may vary. Contact us for the most current availability on this product.
Create contemporary group seating the whole family will enjoy with this upholstery series. Marked by tapered wood legs and arched track arms, this plush seating exudes a casual-contemporary style fit for a wide-range of home decor. In addition, matching toss pillows add an extra layer of comfort and style. Choose from a versatile selection of modular components to create the configuration that is right for you and your family.
The Gulliver collection is a great option if you are looking for furniture in the Worcester, Boston, MA, Providence, RI, and New England area.
These images are representative of this group as a whole and may differ from this item.
Browse other items in the Gulliver collection from Rotmans in the Worcester, Boston, MA, Providence, RI, and New England area.
This item has been tagged as: Sectional, Couch, Sofa, Large Couches, Large Sofa, Sectional Couch, Sectional Sofa, Chaise Sectional, Sectional with Chaise, Couch with Chaise, Sofa with Chaise, Chaise Sofa, Chaise Couch, Sectional with Recliner, Recliner Sectional, Reclining Sectional, Modular Sectional, Modular Sofa Pieces, Stand Alone Couch Pieces, Curved Sectional, Conversation Sofa, Conversational Sofa, Sectional Furniture, Home Theatre Sectional, Home Theater Sectional, Sectional with Ottoman
Nothing on this page may be copied or reproduced without explicit permission.
Drag anItem Here
To start a new blank room plan, enter the desired room dimensions below and click Create Room.
To request a new password, enter your username or e-mail address and click submit. A new password will automatically be emailed to you.
If you are still having trouble logging on after receiving your new password, please email email@example.com


text:
Need Php developer
- Status Closed
- Budget ₹1500 - ₹12500 INR
- Total Bids 10
Project Description
i have coding in php, html, mysql with back end coding in php. code is converted from zend and need to remove errors from programs. Guessing around 100 Fattle and Parse error in all the programs. removing error is one task and build new functionality is 2nd task.
find the attachment with errors, php files of frontend and back end as well. facing same kind of problems in other programs.Get free quotes for a project like this
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online