text:
Property Details
House, 4 Bedrooms, 3.5 Baths, 8 Sleeps.
This new house is a beautiful 3 floor home by the bay
with 4 bedrooms and 3.5 baths.**** There are two large
balconies with the sunset visible from the 3rd floor balcony and a
44-feet private dock space. It sleeps 8 people very comfortably. Relax on the patio. The home is located near the black water sound on the gulf coast side.
balconies accessible from the living room, dining room and bedrooms
via sliding door. Enjoy the beautiful view overlooking the bay and
ocean to spend calm nights with an inviting, delightful sunset in
this spacious new home on the bay.
Just 40-45 minutes from the Miami International Airport, it is
a very convenient location for travelers. The main house consists of
3 bedrooms and 2 ½ baths with one guest bedroom and one full
bathroom (on the first floor). The guest room has a separate central
air. A private boat slip is located in the back yard for sailing and
fishing pleasure. There are also many activities available to engage
in throughout the surrounding area. Take pleasure sailing, fishing,
boating, and swimming from your own backyard and the surrounding
The back patio is beautifully decorated with ceramic tiles
and complete with patio furniture and a hammock for your resting
pleasure so enjoy the view and beautiful twilight!
Recent Review
Lovely property, lots of space!
About the Owner
3 Reviews
5/5 -
(3 traveler reviews)
Lovely property, lots of space!
Nice home in Key Largo, well maintained and clean. Very spacious with a large kitchen - dining area, the bedrooms are nice and large with comfortable beds and plenty of storage. The location is very central to the Key Largo shops and restuarants but it's on a nice quiet street and canal. The owner is a pleasure to deal with. I would definately stay there again.
Great home away from home!
Wonderful home in Key Largo! This house is very nice and is in a great location with lots to do. It is well maintained, well equipped and clean. Also has a great layout, big kitchen, and the rooms and bathrooms were perfect. Our family of 6 was very comfortable staying here. Being able to keep our boat docked and have easy access to the ocean was priceless. The owners are great... very nice and helpful. We had a great time, and wished we could have stayed longer :) Will definatly stay again!
Absolutely love the house! Highly recommend !!!!
We have rented this house twice and absolutely love it! It is one of the biggest on the block with the balcony view of the water canal. It has the dock for jetski and backyard with grill. Inside there are 4 bedrooms, a lot of bathrooms, master bath has jacuzzi. Overall, house has plenty of space. The owner is great and thoughtful of the guests needs. We like to visit Key Largo because it's a very laid back place with still plenty to do outside: fishing, kayaking, snorkeling etc. It's close to Miami for the night life and 2 hours of driving to Key West. Highly recommend this house for vacation!
Activity and Interests
Experience other water-fun activities such as diving,
snorkeling nearby the renowned Coral Reef, swimming with the
dolphins and jet-skiing (rentals accessible). One half mile to JOHN PENNEKAMP.
Underwater State Park and the Glass Bottom Boat Ride, markets,
restaurants, and a variety of shops are just a few other attractions
and necessities included into this extensive and charming vacation
packet. Enjoy the leisure of this tropical paradise and escape to
this sun-filled environment as you relax and tour key largo on your
vacation days.
Rates & Availability
Rental rates
Additional information about rental rates
Monthly Rate: $2700/ for least 6 month staying flat rate. Not including cost of utilities and others.
For more information contact the Owner.
A $170.00 /$ 195, cleaning fee will be deducted from your
6 months to a year rental are available. A $160.00 cleaning fee will be deducted from your
Owner's Cancellation Policy
- 12
- Available arrival
- 12
- Available stay (no arrival)
- 12
- Unavailable
Property Type
- house
- Adventure
- Budget
- Family
- Luxury
- Romantic
- Air Conditioning
- Clothes Dryer ...
- Hair Dryer
- Internet
- Iron & Board
- Linens Provided ...
- Living Room
- Parking ...
- Towels Provided ...
- Washing Machine ...
- Coffee Maker
- Dishes & Utensils
- Dishwasher
- Kitchen
- Microwave
- Oven
- Refrigerator
- Stove
- Toaster
- Dining Room
- Satellite / Cable ...
- Stereo
- Television ...
- pets considered
Location Type
- Beach View
- Water View
- Waterfront
- bay/sound
- churches
- cinemas
- festivals
- library
- marina
- playground
- recreation center
- reefs
- restaurants
Leisure Activities
- eco tourism
- scenic drives
- sight seeing
- walking
Sports & Adventure Activities
- cycling
- fishing
- golf privileges optional
- jet skiing
- pier fishing
- sailing
- scuba diving or snorkeling
- snorkeling
- swimming
- water skiing
- wind-surfing
Floor Area
- 2200 sq. ft.
- Bathroom 1
- Bathroom 2
- Bathroom 3
- Half Bathroom
- 4 Bedrooms, Sleeps 8
- Bedroom 1
- Bedroom 2
- Bedroom 3
- Bedroom 4
- Balcony ...
- Outdoor Grill ...
- Guests provide their own meals
- General: air-conditioning Alarm-Clock Ceiling-Fans central heating Cold-Water-Dispenser linens provided Local-Activities-Guide Paper-Towels Restaurant-Dining-Guide Toilet-Paper towels provided Whirlpool Kitchen: Blender clothes dryer coffee maker cooking utensils dish washer freezer grill Ice-Maker microwave oven refrigerator Stove-Top-Burners toaster washing machine Living Room: comfy seating for 6 people Dining: dining area eating seating for 6 people Bedrooms: 4 bedrooms, sleeps a maximum of 12 people Bedroom 1 - King Bedroom 2 - Queen Bedroom 3 - 2 Double Bedroom 4 - Quen/ Sofa Bathrooms: 3 full baths, 1 half bath Bathroom 1 - Toilet, Tub, Shower Bathroom 2 - Toilet, Combination Tub/Shower Bathroom 3 - Toilet, Shower Bathroom 4 - Toilet Entertainment: Wireless Internet (optional) audio tape player cd DVD radio Basic cable stereo system TV Outside: balcony Deck-Wood Dock-BoatSlip garage Outdoor-Grill-Gas parking Parking-On-Street Walkway-to-Water Patio and balcony furniture


text: An avid investor since the age of 17, Joshua Norman is currently a graduate student at Bentley University's McCallum Graduate School of Business and is a Graduate Department Assistant with Bentley's Hughey Center for Financial Services. Joshua has a fresh perspective and a passion for making money. He has a track record of identifying value stocks that outperform the market.

subset: commoncrawl


text:
Programs and Services
Daily Drop-in
The center houses the Returning Women's Café, a multifunction drop-in lounge, the Alice Miller computer lounge and a resource center. Coffee and tea are available during regular office hours.
Fall 2016 Hours
Monday–Thursday: 10 a.m. – 6 p.m.
Friday: 10 a.m. – 4 p.m.
Additional hours are available upon request. Please call to confirm hours when school is not in session.
Crisis Intervention
The center provides crisis intervention and advocacy services to Brooklyn College students on academic- or nonacademic-related issues.


text:
Arturo Gatti net worth: Arturo Gatti was a retired professional boxer who had a net worth of $3 million dollars. Born in Casino, Italy, Arturo Gatti grew up in Canada, and was in the process of training for the Canadian National Team in 1992, when he decided to turn professional instead. He won the majority of his early fights and in 1995, he signed a deal with HBO to fight his bouts on HBO Boxing. He won all of his fights in 1996 and 1997. Ring Magazine nominated two of his bouts for "Fight of the Year". He moved from Super Lightweight to Lightweight in 1998 and lost all of his fights that year. He won his one fight in 1999, and then began 2000 with a highly controversial win over Joey Gamache who suffered permanent brain damage during the match and was never able to box again. He won the rest of his fights in 2000. In 2001 he went up a weight class unsuccessfully, so he dropped back down after one loss to Oscar de la Hoya. He fought for the next six years with varying success, and chose to retire in 2007. He died in Brazil while on vacation with his wife and child under mysterious circumstances in 2009. His death is still under investigation. During his career, he won 40 of his 49 career fights, 31 by knock out.
Arturo Gatti Net Worth
Arturo Gatti
Professional Boxer
Date of Birth
Apr 15, 1972 - Jul 11, 2009 (37 years old)
5 ft 7 in (1.71 m)
148 lbs (67 kg)
More Boxers
All net worths are calculated by applying a proprietary algorithm. The results are then fact checked and confirmed by a team of editors. We scan all publicly available data and resources and work diligently to ensure that our numbers are the most accurate and up to date net worth data you will find anywhere on the internet.


text:
re: Terrence Jones has agreed to one-year deal with Pelicans - Catdaddy13 on 7/14/16 at 10:50 am
A 24 year old with history of averaging over 12 ppg for a playoff team taking the league minimum to come off the bench for the pels? There has to be another move coming... If he does for a low contract props to the front office for sure imo...Jump to Post - View Topic - Replies (1)
re: Trade for saric - Catdaddy13 on 5/18/16 at 2:09 pm
"Saric would be tied to his rookie-scale deal if plays in the NBA next season. Utah Jazz forward Trey Lyles, who was selected 12th overall in 2015, will make $10.4 million during his four-year rookie contract. Saric will make a little more than that if he comes over this summer. He would be able to...Jump to Post - View Topic - Replies (0)
Trade for saric - Catdaddy13 on 5/18/16 at 1:36 pm
Sixers could be looking for a veteran help. Simmons and Ingram seem to play a simalar style to saric. Does anyone think that pels would be giving up too much if they gave up tyreke and #6 to get saric and maybe another late draft pick in the first?...Jump to Post - View Topic - Replies (8)
re: should have kept dawson - Catdaddy13 on 7/5/15 at 1:25 am
Seriously although this dude is being a little negative for my taste and I trust dell.... For $600,000 which is what we got for Dawson.... Idk if we could have found another 12 or 13th man that could learn to be that high energy versitile defender that Dawson could potentially be... Did the move hel...Jump to Post - View Topic - Replies (0)
re: We should go after Tom izzo - Catdaddy13 on 5/21/15 at 9:27 pm
[quote]People have given you all kinds of reasons. You don't want to listen to anyone though. That's cool. But you should have just started a "We should go after Izzo" thread. [/quote] There you go changed the title for ya... but you have not given me a reason and I cannot tell if you would not w...Jump to Post - View Topic - Replies (0)
re: We should go after Tom izzo - Catdaddy13 on 5/21/15 at 1:18 pm
[quote]Probably because he's mentioned after every offseason and has never left Michigan State. Why mention him again when there are other coaches to mention?[/quote] I think if calapari is and Donavan would have been on our wish list then izzo should be right there with them... He's never ruled ...Jump to Post - View Topic - Replies (1)
re: We should go after Tom izzo - Catdaddy13 on 5/21/15 at 11:47 am
[quote]He is 60 years old and has it made at Mich State, so not sure why he would leave, but I would listen. [/quote] I know age would be somewhat of an issue, but I just don't see how he hasn't gotten as much mention after every other offseason in the past him being mentioned and at least listen...Jump to Post - View Topic - Replies (2)
We should go after Tom izzo - Catdaddy13 on 5/21/15 at 10:24 am
All of the coaching lists involve coaches such as Van Gundy, hoiberg, calapari, Thibodeau, and other assistants that everyone can make a case for. Why no love for Tom Izzo. Every year Izzo's name is brought up, and it seems that he is willing to listen. I think his teams are always playing a physica...Jump to Post - View Topic - Replies (3)
re: My dark horse Coach - Catdaddy13 on 5/19/15 at 2:15 pm
Van gundy was always #2 on my list for pels coaches... #1 is someone also that has been waiting for the right opportunity, and that is TOM IZZO... I don't see how people are not talking about him being a very high target.... I know he has turned down many different coaching jobs, but he has never go...Jump to Post - View Topic - Replies (1)
re: New Name for DC Search: Don Pellum - Catdaddy13 on 1/8/15 at 8:08 am
Posted on Coaching ChangesJump to Post - View Topic - Replies (0)
Andrew luck - Catdaddy13 on 12/25/14 at 12:36 pm
Anybody thinking pagano will play Luck or starters for more than a half? I've rode the luck to hilton connection all year... I'm staying away from Hilton this week, but thinking that luck could at least have a better chance of putting up a couple touchdowns on the titans rather than trying to rely o...Jump to Post - View Topic - Replies (1)
Week 17 leagues - Catdaddy13 on 12/23/14 at 8:20 am
Week 17 leagues I know you are all out there playing to the final whistle!!! With that being said... Please tell me there's a chance!.. Who's sitting or am I screwed because it's week 17 and basically 3 games with teams not trying to suck? I am down 29.1 with: QB Andrew Luck, @Ten RB Le'Veon ...Jump to Post - View Topic - Replies (0)
re: Is TY Hilton in or out? - Catdaddy13 on 12/21/14 at 2:10 pm
Let's go moncrief!!!!...Jump to Post - View Topic - Replies (1)
re: Do we wait for T.Y.'s status for the 3:30 game? or start a nooner? WWYD? - Catdaddy13 on 12/19/14 at 6:24 pm
I've got the ty AND Julio delima I already have Harry d for Julio if he is out... I just don't know if I should trust moncrief over Shane Vareen, Charles Johnson, or even Jordan reed who I think is due(for no reason) at the flex.. Now I'm just typing my thoughts... Championship week getting to my h...Jump to Post - View Topic - Replies (1)
Moncrief if ty sits - Catdaddy13 on 12/19/14 at 12:44 pm
Do I trust him just like I trust Harry Douglas if Julio sits... Yes I have both Julio and Ty for the ship It's either Vareen, Charles Johnson, or moncrief at the flex if he sits.... Just don't know when they announce Ty's status for the 3pm kickoff...Jump to Post - View Topic - Replies (0)
re: Post your championship match ups for all to see! - Catdaddy13 on 12/17/14 at 12:48 pm
QB Andrew Luck, Ind QB @Dal RB Le'Veon Bell, Pit RB KC RB Tre Mason, StL RB NYG WR T.Y. Hilton, Ind WR Q @Dal WR Josh Gordon, Cle WR @Car TE Jimmy Graham, NO TE Atl FLEX Julio Jones, Atl WR Q @NO D/ST Bills D/ST D/ST @Oak K Dan Bailey, Dal K Ind Vs QB Ben Roethli...Jump to Post - View Topic - Replies (0)
re: Teams that will be resting starters - Catdaddy13 on 12/16/14 at 3:03 pm
Gone all year without a backup behind luck... Rg3, Andy dalton, or Alex smith are looking like they will b the only options if luck sits week 17 hopefully I can get a pretty big cushion from week 16 points...Jump to Post - View Topic - Replies (1)
Teams that will be resting starters - Catdaddy13 on 12/16/14 at 8:43 am
I'm in a league that we unfortunately play all 17 weeks... I've been watching the colts record as I have the dominate Luck and Hilton connection.. Pagano has been quoted saying that they will be playing to win... I'll see how much they are trying to win this week and start the players that have been...Jump to Post - View Topic - Replies (2)
Seantavious Jones - Catdaddy13 on 12/12/14 at 9:19 am
Any word if he can help the team? I remember hearing about him during preseason, but did not watch him while looking for Brandon Coleman to make the 53 instead....
Posted on Saints TalkJump to Post - View Topic - Replies (1)
re: Trading for demarco Murray - Catdaddy13 on 11/11/14 at 12:20 pm
Jeez tigerdroppings has really cleaned up... I would never thought after all these years of spying on the rant crowd that everyone would be so ethical!! Like I said earlier I was offering, and now it is probably declined in which it should be with all playoff seeding and collusion aside... I guess t...Jump to Post - View Topic - Replies (0)
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text:
Tuesday, Oct. 27, 2009 | Housing prices in San Diego County rose again in August, rounding out a summer of monthly price gains, according to data released Tuesday.
Prices rose a seasonally adjusted 1.52 percent between July and August, the Standard & Poor's/Case-Shiller home price index showed.
Though prices in August were still down 8.85 percent from the same month in 2008, that year-over-year gap has been shrinking, reaching single digits for the first time in nearly two years. Those gaps were larger than 20 percent for more than a year of the downturn.
"It seems to me that it is the process of turning around," said Lynn Reaser, president-elect of the National Association for Business Economics and chief economist at Point Loma Nazarene University. "The lower end in particular seems to have turned a corner."


text:
Well! I think it's about time I shared my thoughts on this little product, Hydraluron.
Unless otherwise indicated products reviewed are press or brand samples. Links may be affiliate links.
Facebook Conversations
7 Responses to Hydraluron Review
- British Belle November 14, 2012 at 12:44 pm #
This sounds so good …I'm so tempted to try it!
Eysilix is fab. It really does work but it reacted badly to my skin 🙁
Laura xo
- Beauty Daze November 20, 2012 at 12:20 am #
Glad you like it, plus you've helped me understand how it works from a practical non-science crap perspective. Boots have a deal on atm, buy any Indeed Labs product and get Peptalash free! Go.buy Eysilix now, I think the offer ends Nov 24th! I'm getting Hydraluron 😀
Steph xx
- Ree ReallyRee November 20, 2012 at 9:23 am #
ha ha!! I can't do science!!! I have the Eysilix. Trying it now. Oooh I haven't tried Peptalash. Be interested to hear what you think xxx
The free Peptalash deal is still on at Boots, plus it's glitching so if you buy more than one, money comes off, making it really cheap! I've explained how the glitch works better in my blog http://www.sundaycakesuk.blogspot.co.uk so have a look if you're planning on buying any more. 🙂


text:
Baseline regulatory T cell (Treg) characteristics in subjects with multiple sclerosis (MS). A, Representative Treg coculture assay [3H] thymidine incorporation data (mean counts per minute [standard deviation]) showing dose-dependent Treg suppression for a subject with MS (86% suppression). Up to 5 × 103 fluorescence-activated cell sorter (FACS) sorted CD4+ CD25high (Treg) cells were titrated in coculture with 5 × 103 CD4+CD25− (responder) cells for 5-day stimulation with anti-CD3 antibody. B, Baseline Treg suppression in subjects with MS (mean [SD], 69.7% [18%]) compared with healthy donors (HD) (mean [SD], 79.7% [13%]; P = .27). C, Frequency of peripheral blood CD4+Foxp3+ cells from subjects with MS (mean [SD], 2.7% [1.6%] of lymphocytes) compared with age-, race-, and sex-matched HD (mean [SD], 3.2% [1.0%] of lymphocytes; P = .37). D, Representative CD4+ gated FACS plots from a subject with MS showing negative CD69 and low or negative CD127 expression in forkhead box P3 (Foxp3+) cells (data representative of 6 subjects with MS and 3 HD), and attenuation of interleukin (IL)-2 production following 6-hour phorbol myristate acetate/ionomycin stimulation (data representative of 4 subjects with MS and 3 HD).
Reduction in regulatory T cells (Treg) during anti-CD25 antibody treatment. A, Anti-CD25 antibody saturation and total CD25 expression on lymphocytes over time. Antibody saturation and CD25 expression were evaluated by fluorescence-activated cell sorter (FACS) analysis of whole blood using 2 fluorochrome-labeled antibodies (Anti-Tac and 7G7) directed at noncompeting epitopes on CD25. Complete antibody saturation of CD25 was demonstrated by lack of fluorochrome-labeled Anti-Tac binding. Total CD25 expression (7G7) declined by 13% (P < .001, repeated-measures analysis of variance; n = 12). Months 0 and 0.5 represent the first 2 infusions. Monthly administration followed thereafter, with month 13.5 representing the final infusion. B, Antibody saturation (Anti-Tac) and CD25 expression (7G7) in cerebrospinal fluid (CSF) lymphocytes over time (P = .04; n = 10). C, Histograms show representative signal transduction and activator of transcription 5 phosphorylation (pSTAT5) in peripheral blood mononuclear cells obtained during anti-CD25 antibody treatment (bold line) compared with baseline (thin line) and control (shaded) following 15-minute ex vivo interleukin (IL)-2 exposure. The box plot compares pSTAT5 during treatment compared with baseline (P = .005; n = 7). D, Representative CD4+ gated FACS analysis of CD4+CD25+ forkhead box P3 (Foxp3) conventional activated T cells (upper left quadrants) and CD4+CD25+Foxp3+ Treg (upper right quadrants) at baseline and during treatment. E, Reduced Foxp3 mean fluorescence intensity during treatment compared with baseline (P < .001; n = 12). F, Reduction in frequency of CD4+Foxp3+ cells over time during the course of anti-CD25 antibody treatment (P < .001, repeated-measures analysis of variance; n = 12). The dashed line indicates the end of treatment.
Regulatory T cell (Treg) in vivo proliferation and ex vivo suppression are impaired during anti-CD25 antibody treatment. A, Representative fluorescence-activated cell sorter (FACS) analysis comparing Ki67 expression in forkhead box P3 (Foxp3+) cells at baseline and during anti-CD25 antibody treatment (the percentage of Ki67-expressing cells is in parentheses). The bar graph compares the mean (SD) proportion of CD4+Foxp3+ cells expressing Ki67 at baseline and during treatment (P = .001; n = 12). B, Representative CD4+ gated FACS analysis showing that most CD4+Foxp3+ cells (blue) are CD25+CD127low/neg (polygonal gate) at baseline and remain CD25+CD127low/neg during treatment. The numbers indicate the frequency of cells that are Foxp3+ within the polygonal gate and, in parentheses, the percentage of total lymphocytes that are CD4+Foxp3+ cells. C, Representative [3H] thymidine incorporation data (mean [standard deviation] counts per minute) shown for up to 5 × 103 FACS-sorted Treg (CD4+CD25+CD127low/neg) titrated in coculture with 5 × 103 responder (CD4+CD25−CD127+) cells. Closed circles represent cells from baseline and open circles represent cells obtained during anti-CD25 antibody treatment. D, Reduced suppressive capacity (mean [standard deviation] percentage of suppression) of circulating Treg during anti-CD25 antibody treatment (P = .03; n = 4).
Lack of correlation between reduction in the frequency of regulatory T cells (Treg) and changes in acute central nervous system inflammatory activity. A, Reduction in brain inflammatory activity indicated by reduction in the total number of contrast-enhancing multiple sclerosis (MS) lesions per month (total number of gadolinium dithylenetriamine pentaacetic acid [Gd-DTPA] lesions) on serial brain magnetic resonance images (P < .001; n = 13). B, Regression analysis of correlation between change in total number of contrast enhancing lesions per month (percentage of reduction in Gd-DTPA+ lesions) and change in the frequency of circulating Treg (percentage of reduction in the number of Treg) at month 7.5 during anti-CD25 antibody treatment (r2 = 0.0171; P = .68, Pearson correlation coefficient). C-F, Analysis of Treg in lesional skin. C, Hematoxylin-eosin (H&E)–stained tissue section (subject MS 9) showing epidermal changes of the skin lesion, characterized by compact hyperkeratosis, acanthosis, and focal spongiosis with exocytosis of lymphocytes (magnification ×20). D, H&E-stained section showing histologic changes in the superficial dermis, characterized by a perivascular chronic inflammatory infiltrate comprised predominantly of lymphocytes. E, Immunohistochemical staining for CD3. F, Immunohistochemical staining for forkhead box P3 (Foxp3).
Oh U, Blevins G, Griffith C, Richert N, Maric D, Lee CR, McFarland H, Jacobson S. Regulatory T Cells Are Reduced During Anti-CD25 Antibody Treatment of Multiple Sclerosis. Arch Neurol. 2009;66(4):471-479. doi:10.1001/archneurol.2009.16
© 2017
Maintenance therapy with anti-CD25 antibody has emerged as a potentially useful treatment for multiple sclerosis (MS). Constitutive CD25 expression on CD4+CD25+ regulatory T cells (Treg) suggests that anti-CD25 antibody treatment may potentially target a subset of T cells that exhibit immune suppressive properties. We examined changes to CD4+CD25+ Treg in patients with MS receiving maintenance anti-CD25 monoclonal antibody treatment to determine the effect of treatment on Treg and, consequently, on immunological tolerance.
Peripheral blood and cerebrospinal fluid samples obtained from a before-and-after trial of anti-CD25 antibody monotherapy were examined to compare baseline and treatment differences in CD4+CD25+ Treg.
A total of 15 subjects with MS. One subject was withdrawn owing to an adverse effect.
Sustained reduction of the frequency of CD4+CD25+ Treg was observed during treatment. Anti-CD25 antibody treatment led to evidence of impaired in vivo Treg proliferation and impaired ex vivo Treg suppression. Inflammatory MS activity was substantially reduced with treatment despite reduction of circulating Treg, and there was no correlation between changes in the frequency of Treg and changes in brain inflammatory activity. However, new-onset inflammatory disease, notably dermatitis, was also observed in a number of subjects during treatment.
The reduction in Treg did not negatively affect maintenance of central nervous system tolerance during anti-CD25 antibody treatment. The incidence of new-onset inflammatory disease outside of the central nervous system in a subset of patients, however, warrants further studies to examine the possibility of compartmental differences in the capacity to maintain tolerance in the setting of reduced CD4+CD25+ Treg.
The anti-CD25 monoclonal antibody daclizumab targets the α subunit of the high-affinity interleukin (IL)-2 cytokine receptor complex. The upregulation of CD25 following T cell activation and the subsequent IL-2 signaling constitutes a key event in T cell clonal expansion and differentiation. Abnormalities of the IL-2–CD25 cytokine pathway have been reported in a number of immune-mediated diseases including multiple sclerosis (MS) and suggest that CD25 is a potential target for MS immunotherapy. Increased soluble CD25 levels and abnormally high IL-2 responsiveness of autoreactive T cells in subjects with MS implicate an aberrant IL-2/CD25 circuit in the pathogenesis of MS, and constitute the rationale for anti-CD25 antibody treatment to modulate IL-2 signaling in MS.1,2 A number of clinical studies are beginning to demonstrate the immunomodulatory effect of the anti-CD25 monoclonal antibody daclizumab in subjects with MS.3,4
Experimental evidence of the past decade has made increasingly clear that a subset of CD25-expressing CD4+ T cells demonstrate suppressive or regulatory properties and contribute to the maintenance of immunological self-tolerance by their inhibitory influence on autoreactive T cells.5 These CD4+CD25+ regulatory T cells (Treg) are distinguished from conventional activated T cells by constitutive high expression of CD25 and by the expression of Treg lineage specification factor forkhead box P3 (Foxp3).6,7 Conventional activated T cells, by contrast, express intermediate levels of CD25 and lack Foxp3.6,8 Whereas conventional activated T cells coordinate and amplify immune responses, CD4+CD25+ Treg actively suppress immune responses, including those involved in autoimmunity.9 The development of multiorgan inflammatory disease following Treg depletion indicates that CD4+CD25+ Treg make a critical contribution to the maintenance of immunologic self-tolerance.10 The loss or dysfunction of CD4+CD25+ Treg has been implicated in the pathogenesis of a growing number of disorders including systemic lupus erythematosus,11 psoriasis,12 aplastic anemia,13 and MS,14 suggesting a potentially broad relevance with respect to human autoimmune diseases.
The shared expression of CD25 on conventional activated T cells and CD4+CD25+ Treg suggest that both are potentially targeted by anti-CD25 antibody. Based on the knowledge that CD4+CD25+ Treg contribute to maintenance of tolerance, an inhibitory effect on Treg could potentially exacerbate existing inflammatory disease or unmask underlying predilection for new inflammatory disease. We therefore examined the changes to the CD4+CD25+ T cell subsets in subjects with MS undergoing anti-CD25 antibody treatment. In particular, we asked what effect an antihuman CD25 antibody has on CD4+CD25+ Treg, whether changes to CD4+CD25+ Treg affected the immunomodulatory effect of treatment, and whether changes to CD4+CD25+ Treg affected maintenance of overall immunological tolerance.
Subjects with MS15 were enrolled in an open-label trial of anti-CD25 antibody (daclizumab). Subjects were free of immunomodulatory therapy for 24 weeks prior to enrollment and received intravenous infusion of daclizumab monotherapy (1 mg/kg) every 4 weeks for 54 weeks. Peripheral blood was obtained at baseline and during treatment. Cerebrospinal fluid was obtained at baseline and during treatment. Whole blood was processed immediately for fluorescence-activated cell sorting (FACS) analysis. Peripheral blood mononuclear cells (PBMC) available from 12 subjects were processed by Ficoll-Hypaque density centrifugation and cryopreserved for later use. All treatment samples were obtained just prior to administration of treatment (trough sample). Unless otherwise stated, baseline PBMC samples were compared with treated PBMC samples obtained at month 2.5 (trough sample following third dose). Brain magnetic resonance images were obtained monthly as previously described.3 Informed consent was obtained from each subject. The study was reviewed and approved by the National Institute of Neurological Disorders and Stroke institutional review board.
The following antibodies were used according to manufacturer's instructions: CD3, CD4, CD8, CD25 (M-A251), CD56, CD127, IL-2, Ki67, and pSTAT5 (signal transduction and activator of transcription 5) antibodies were obtained from BD Biosciences (San Jose, California). The Foxp3 phycoerythrin (PE) or allophycocyanin antibodies were obtained from eBioscience (San Diego, California). The CD25 (Anti-Tac) fluorescein isothiocyanate conjugate was from Immunotech (Westbrook, Maine). The CD25 (7G7) PE was from Ancell (Bayport, Minnesota). The FACS analysis of surface markers were performed on erythrocyte-lysed washed whole blood samples. The PBMC were used for all other analyses. Green Dead Stain (Invitrogen, Carlsbad, California) was used for live or dead cell discrimination with Foxp3 staining. Flow cytometric data was acquired on FACSCalibur (BD Biosciences) and analyzed on FlowJo (TreeStar, Ashland, Oregon).
To compare Treg suppression between patients with MS at baseline and healthy donors, CD4+ T cells were purified from PBMC by negative selection magnetic beads (Miltenyi, Auburn, California). Purity was more than 95%. The CD25-PE–labeled CD4+ T cells were then sorted (FACS diva; BD Biosciences) into CD4+CD25high Treg (highest, 3% CD25 expression) and CD4+CD25− responder cells. The Treg coculture suppression assay was performed as previously described,8 with minor modifications. Soluble anti-CD3 (HIT3a, 0.1μg/mL; BD Pharmingen, Franklin Lakes, New Jersy) was used for stimulation. Irradiated (3000 rad) T cell–depleted (MACS [magnetic activated cell sorting] cell separation/anti-CD3 microbeads; Miltenyi) autologous PBMC was used as accessory cells. Cells were plated in triplicate into 96-well plates, incubated at 37°C for 5 days, and pulsed with [3H] thymidine for the final 18 hours of incubation. The percentage of suppression was calculated as %suppression = [1−(cpm of coculture well/cpm of responder well)] × 100, where cpm indicates counts per minute, and the coculture well ratio was 1:1 (responder, Treg). To compare the baseline and treatment Treg suppression, CD4+ T cells were purified from viably cryopreserved PBMC by negative selection magnetic beads. Purified CD4+ T cells were labeled with CD127-PE and CD25-PE/Cy5 antibodies. The CD4+CD25+ CD127low/neg (Treg) and CD4+CD25−CD127+ (responder) cells were sorted by FACS and used for Treg coculture suppression assay as described above.
Formalin-fixed paraffin-embedded tissue sections were prepared on poly-L-lysine coated slides. Immunohistochemistry for CD3 (Dako, Glostrup, Denmark) and Foxp3 (Abcam, Cambridge, Massachusetts) was carried out on consecutive tissue sections and developed with 3,3′-diaminobenzidine, tetrahydrochloride chromogen. A semiquantitative assessment of CD3 and Foxp3 expression was carried out by counting the number of positively stained lymphocytes under an objective microscopy lens at magnification ×40.
Statistical significance was determined by unpaired t test to compare subjects with MS with healthy donors and by paired t test to compare baseline and treatment values. Where appropriate, comparisons were made using general linear model repeated measures analysis of variance. Pearson correlation coefficients were used to analyze relationships between parameters.
To establish pretreatment characteristics of CD4+CD25+ Treg in this cohort of subjects with MS, Treg suppression was measured by an in vitro Treg coculture assay8 (Figure 1A). Most subjects in this cohort demonstrated baseline Treg suppression within the range for healthy donors (P = .27) (Figure 1B). Likewise, the frequency of circulating CD4+Foxp3+ Treg in this cohort of subjects with MS did not differ significantly at baseline compared with that of age-, sex-, and race-matched healthy donors (P = .37) (Figure 1C). Furthermore, as in healthy donors, Foxp3+ cells from subjects with MS were characterized by negative CD69 and low or negative CD127 expression16,17 and demonstrated attenuation of IL-2 production6,18 (Figure 1D).
Antibody saturation was monitored during the course of anti-CD25 antibody treatment by flow cytometry using 2 fluorochrome-labeled antibodies (anti-Tac and 7G7) that bind noncompeting epitopes on CD25. Complete antibody saturation of CD25, demonstrated by the absence of fluorochrome-labeled anti-Tac binding, was maintained during the course of treatment (Figure 2A, anti-Tac), whereas reduction in the mean total CD25 expression on lymphocytes was less pronounced (13%) but nevertheless statistically significant (P < .001) (Figure 2A, 7G7). Examination of cerebrospinal fluid demonstrated complete antibody saturation of CD25 on cerebrospinal fluid lymphocytes and a 20% decline in mean total CD25 expression during treatment (P = .04) (Figure 2B).
Signaling of IL-2 was inhibited by anti-CD25 antibody treatment. STAT5 phosphorylation, which mediates downstream IL-2 signaling, was used as a marker of IL-2 signaling. Lymphocytes obtained during treatment demonstrated nearly complete absence of STAT5 phosphorylation in response to low-level (10 U/mL) IL-2. Significant reductions in STAT5 phosphorylation were also observed at higher levels (50 and 100 U/mL) of IL-2 (P = .005) (Figure 2C).
The effect of anti-CD25 antibody treatment on CD4+CD25+ Treg was analyzed by examining Foxp3 as a marker of Treg. Flow cytometric analysis (Figure 2D) demonstrated a reduction in mean fluorescence intensity of Foxp3 expression during treatment compared with baseline (P < .001) (Figure 2E). Reduction in Foxp3 expression at the single cell level during treatment is consistent with previous studies implicating STAT5 as a regulator of Foxp3 gene transcription.19 Furthermore, the frequency of total Foxp3-expressing CD4+ cells were reduced, with approximately 30% reduction in the mean frequency of CD4+Foxp3+ cells observed by month 2.5 and 44% reduction by month 7.5 (P < .001) (Figure 2F). Similar reductions were observed in the frequency of CD4+CD25+Foxp3+ cells (45% reduction; P < .001). Posttreatment samples available from a limited number of subjects demonstrated recovery of Treg frequencies to near baseline levels.
Based on the known role of IL-2 in promoting cell cycle progression in conventional T cells,20 we asked whether altered homeostatic proliferation of Treg could account for the reduction in frequency of Treg during treatment. The effect of anti-CD25 antibody treatment on Treg proliferation was examined using Ki67 expression to estimate the in vivo proliferating fraction,21 determined as the proportion of CD4+Foxp3+ cells expressing Ki67. The PBMC from baseline and treatment were stained ex vivo for intracellular expression of Ki67. Consistent with a previous study demonstrating high in vivo proliferative kinetics of human Treg,22 CD4+Foxp3+ cells demonstrated high Ki67 expression at baseline compared with total CD4+ cells (mean [SD], 11.7% [2.2%] vs 2.13% [0.8%], respectively). Analysis of treatment samples showed a reduction in the Ki67-expressing proliferating Treg fraction (P < .001) (Figure 3A), suggesting impaired homeostatic proliferation of Treg during anti-CD25 antibody treatment.
To assess Treg function, coculture suppression assays were performed to compare Treg suppression at baseline and during treatment. Because of altered CD25 expression during treatment, an additional surface marker, CD127, was used to sort Treg, which express low or no CD127 (CD127low/neg).17 The FACS analysis demonstrated that most CD4+Foxp3+ cells were contained within the CD25+CD127low/neg subset at baseline and during treatment (Figure 3B). To determine the effect of anti-CD25 antibody treatment on Treg suppressive capacity, CD4+CD25+CD127low/neg cells were sorted by FACS from PBMC obtained at baseline and during treatment and cocultured with autologous CD4+CD25−CD127+ (responder) cells. Treatment samples demonstrated impaired Treg suppression compared with baseline samples (P = .03) (Figure 3C and D), indicating a functional impairment of ex vivo Treg suppression during anti-CD25 antibody treatment.
In addition to changes in Treg, anti-CD25 antibody treatment led to significant alteration in conventional activated CD4+CD25+ T cells and CD56bright natural killer (NK) cells. The frequency of conventional activated T cells (CD4+CD25+Foxp3−) was reduced during anti-CD25 antibody treatment (P < .001), which corresponded to the contraction of Ki67+ proliferating fraction of conventional activated T cells. In contrast, the proportion of proliferating CD56+ NK cells was increased during anti-CD25 antibody treatment and corresponded to the expansion of CD56bright NK cells observed during the course of therapy (P <.001).
In the setting of simultaneous changes to activated conventional T cell and NK cell compartments, Treg were not necessarily the major determinants of acute central nervous system inflammation. Contrast (gadolinium dithylenetriamine pentaacetic acid [Gd-DTPA]) enhancement of MS lesions on brain magnetic resonance imaging, a marker of MS inflammatory activity, was assessed on a monthly basis. The number of MS lesions demonstrating Gd-DTPA enhancement (Gd-DTPA+ lesions) was significantly reduced during treatment with anti-CD25 antibody (P < .001) (Figure 4A). To assess whether the reduction in Treg had any negative effect on the immunomodulatory effect of anti-CD25 antibody, we analyzed the relationship between changes in the frequency of Treg and brain inflammatory activity. No significant correlations were observed between changes in the frequency of Treg and changes in brain inflammatory activity measured as the total number of Gd-DTPA+ lesions per month (r2 = 0.0171; P = .69) (Figure 4B) or the number of new Gd-DTPA+ lesions per month (r2 = 0.0157; P = .71) when assessed at month 7.5. Analysis of earlier and later time points (months 2.5 and 12.5) yielded similar results (r2 = 0.068 and r2 = 0.0391, respectively, for correlation between change in total number of Gd-DTPA+ cells and Treg).
Dermatitis occurred in 3 of 15 individuals who were taking anti-CD25 antibody (Table). The onset of dermatitis occurred during anti-CD25 antibody treatment in 2 subjects and at the end of treatment in one subject who nevertheless still demonstrated more than 80% saturation of CD25 at the onset of dermatitis. An additional subject with a family history of rheumatoid arthritis developed palindromic rheumatism during treatment. Reductions in the frequencies of Foxp3+ cells for subjects who developed dermatitis are shown in the Table. Histologic examination of lesional skin from 2 subjects who developed dermatitis during treatment showed spongiotic to psoriasiform epidermal changes with perivascular lymphocytic inflammatory infiltrate in the subjacent superficial dermis (Figure 4C and D). In situ quantitative detection of Foxp3 in the lesional skin showed that approximately 13% of infiltrating CD3+ cells were Treg (Figure 4E and F).
Here we demonstrate that long-term maintenance anti-CD25 antibody treatment led to sustained reduction of CD4+CD25+ Treg in subjects with MS. In contrast to the hypoproliferative nature of Treg in culture, we and others22 find evidence that Treg exhibit high replicative capacity in vivo. Reduced proliferating Treg fraction corresponds to decline in Treg numbers in the setting of impaired IL-2 signaling and suggests that IL-2–supported Treg proliferation accounts for a substantial portion of the human circulating Treg pool. Clinical trials in patients with cancer demonstrated upregulation of Foxp3 and increased frequency of Treg following administration of IL-2.23,24 Our data demonstrates, conversely, that negative perturbation of IL-2 signaling reduces Foxp3 expression and reduces the circulating Treg pool. Collectively, these studies indicate that IL-2 plays a major role in controlling the homeostatic set point for the size of the human circulating Treg pool.
We asked whether reduction in circulating Treg during anti-CD25 antibody treatment negatively affected MS inflammatory activity. Overall brain inflammatory activity was reduced during treatment, suggesting a shift toward tolerance. The lack of a correlation between changes in frequency of Treg and changes in brain inflammatory activity suggests that sustained reduction in circulating Treg did not negatively affect disease activity. One likely explanation is that simultaneous changes in other cell subsets during anti-CD25 antibody treatment countered any negative affect of reduced Treg. Anti-CD25 antibody treatment led to a contraction of the CD4+CD25+ activated conventional T cell fraction and an expansion of CD56bright NK cells. A previous study demonstrated the capacity of CD56bright NK cells to suppress inflammation; expansion of CD56bright NK population is potentially a major determinant of acute brain inflammatory activity during anti-CD25 antibody treatment.25 Alternatively, the lack of correlation between changes in circulating Treg and changes in brain inflammatory activity suggests the possibility that Treg are not a major determinant of acute inflammatory activity in MS. Data from experimental autoimmune encephalomyelitis, an animal model of MS, have not yet reconciled what role CD4+CD25+ Treg play in modulating acute central nervous system inflammation. Loss of Treg appears to confer susceptibility to experimental autoimmune encephalomyelitis in an otherwise resistant strain of mice,26 but central nervous system antigen-specific Treg failed to inhibit central nervous system effector T cells during the acute phase of experimental autoimmune encephalomyelitis, possibly owing to the in situ cytokine milieu, particularly IL-6, that renders effector T cells resistant to Treg suppression.27
Dermatitis occurred in 3 of the 15 subjects receiving treatment. An additional subject with a family history of rheumatoid arthritis developed migratory tenosynovitis during treatment, diagnosed as palindromic rheumatism. The incidence of new-onset inflammatory disease during anti-CD25 antibody treatment raised the possibility that there may be compartmental differences in the capacity to maintain tolerance in the setting of reduced circulating Treg. A recent study showed that CD4+CD25+ Treg contribute to routine immune surveillance and inflammatory response in the human skin.28 Furthermore, the availability of circulating Treg capable of migrating into the skin was shown to be critical to the maintenance of skin-specific tolerance in an animal model, suggesting that the skin may be particularly vulnerable to reduction in circulating Treg.29 Histologic findings from skin biopsies taken from our subjects were relatively nonspecific, but not inconsistent with what has been described in the Foxp3-deficiency syndrome immune dysregulation, polyendocrinopathy, enteropathy, X-linked (syndrome).30 In situ quantitative detection of Foxp3 in the lesional skin showed that approximately 13% of infiltrating CD3+ cells were Treg, which represents a lower frequency of Treg at the site of skin inflammation compared with historical controls.31 Reduction in the frequency of Treg was relatively high (above the cohort mean/median) in subjects who developed new-onset inflammatory disease, but did not reach statistical significance compared with those who did not develop new inflammatory disease on treatment. The relationship between reduction in Treg and new-onset inflammatory disease during anti-CD25 antibody treatment, though suggestive, is inconclusive, and further work is required to determine whether there are compartmental differences in requirements for CD4+CD25+ Treg to maintain organ-specific tolerance.
A functional defect of CD4+CD25+ Treg cells has been reported in subjects with MS.14,32 We found no significant difference in mean Treg suppression between our cohort of subjects with MS and healthy donors, suggesting either that a functional defect of Treg may not be a uniform finding in all subjects with MS or that the differences are on a scale that requires a larger cohort to adequately power such comparisons. Studies comparing Treg suppression in subjects with MS and healthy volunteers suggest that the differences may be age-dependent33 or disease stage–dependent.34
The collective clinical experience with anti-CD25 antibody treatment constitutes a large body of data that demonstrates its safety and efficacy as induction therapy in the prevention of allograft rejection35 and suggest its utility in an array of human disorders.36 The effect of short-term anti-CD25 antibody induction therapy on Treg is likely transient or modified by concomitant use of immunosuppressive agents.37 We now demonstrate that a consequence of long-term maintenance monotherapy with anti-CD25 antibody is a sustained reduction of CD4+CD25+ Treg. Maintenance therapy with anti-CD25 antibody is likely to be a valuable therapeutic option in a number of immune-mediated inflammatory diseases.3,38 Our findings underscore the need to clarify the organ-specific consequences of sustained reduction in human CD4+CD25+ Treg.
Correspondence: Steven Jacobson, PhD, 10 Center Dr, Bldg 10, Room 5C103, Bethesda, MD 20892 (email@example.com).
Accepted for Publication: September 11, 2008.
Author Contributions: Dr Oh had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Oh, Blevins, and Jacobson. Acquisition of data: Oh, Blevins, Griffith, Richert, Maric, and Lee. Analysis and interpretation of data: Oh, Griffith, Richert, McFarland, and Jacobson. Drafting of the manuscript: Oh, Blevins, Griffith, Richert, and Jacobson. Critical revision of the manuscript for important intellectual content: Oh, Blevins, Maric, Lee, McFarland, and Jacobson. Statistical analysis: Oh. Obtained funding: McFarland and Jacobson. Administrative, technical, and material support: Oh, Griffith, Richert, and McFarland. Study supervision: Jacobson.
Financial Disclosure: Dr McFarland reports being coinventor on National Institutes of Health patents related to the use of daclizumab in multiple sclerosis and, as such, received patent royalty payments.
Funding/Support: This research was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Neurological Disorders and Stroke.
Additional Contributions: We thank Joan Ohayon, CRNP, for clinical assistance, and Azita Kashani, BS, for technical assistance.


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online marketing
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- Budget ₹12500 - ₹37500 INR
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We are launching a new travel site in next few weeks. Need a full time/part time person who has good experience in online marketing. Knowledge of SEO is also appreciated. People based out of Mumbai or willing to work out of Mumbai shall apply.Get free quotes for a project like this
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Samuelle Couture
Ivory Light Pink
Ever since her design graced the cover of our SMP Weddings book, I've been wholeheartedly obsessed with Samuelle Couture. Because she doesn't just create dresses. She creates moments. And certainly isn't afraid to break the traditional "wedding dress rules" along the way. We caught up Sam to talk everything from bridal trends to motherhood, and the always amazing KT Merry was on hand to capture. Catch our full Q&A below and find even more photo goodies tucked away right here.
Style Me Pretty: How did you get your start in the bridal industry?
Samuelle Couture: I began when friends asked me to design their wedding dresses. A number of them were artists, people who were looking for something other than the traditional wedding dress. They were also people with a creative eye and openness to collaboration.
SMP: First article of clothing you designed?
SC: As a younger person, the people, the looks and the sounds of London town inspired me. I came of age with Post-Punk – a very creative time in the UK for both music and fashion – and my earliest clothing designs would have been for me and my friends when we travelled into the city to see bands. It all seems a long, long time ago!
SMP: The moment you realized you had "made it" in the industry?
SC: I'm not sure if one ever feels one's made it, it's always a work in process from collection to collection I strive to create looks that stay true to my ideal. I've had some wonderful moments while founding Samuelle Couture, and built some great relationships with creative and like-minded people in the industry. It was very special to recently sell a collection to a notable store and also to have one of my wedding dresses appear on Vogue.com
SMP: The greatest lesson you've learned in business?
SC: I've been careful to grow and nurture Samuelle Couture organically, in a way that I can sustain and develop. It's a learning process, and every business has it's own personality.
SMP: Do you have any predictions for upcoming bridal trends?
SC: I'm not much of a trend follower. I would say that some of each new collection has been inspired by conversations I've had with past brides, as well as watching the beautiful fabrics and detailing in the couture collections. For me, the perfect wedding dress combines the mood of the moment as well as classic couture. It should captivate and inspire the bride and the audience!
SMP: Your advice for selecting that perfect gown?
SC: Don't rush the decision, take your time and allow time.
SMP: How do you manage to balance work and motherhood?
SC: Balancing work and motherhood is a fine balance and quite a challenge! My new son is such a gorgeous beautiful spirit that I want to spend every minute with him. He makes me laugh instantly, and brings a new joy into my home. Designing and making wedding dresses has been my love for so many years, I am trying to nurture both!
SMP: Three things every woman should have in her closet?
SC: An inspired scent, some lovely underwear and a weekend get away bag!
SMP: Words to live by?
SC: Be true to yourself.
Photography: KT Merry Photography | Floral Design: Kat Flower | Reception Venue: Rosewood Mayakoba | Favors: One Girl Cookies | Gown Designer: Samuelle | Vendor: James Galanos


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electrical malfunction occurred when lights making snowflakes were
supposed to transform into the five Olympic rings. While four of the
five lit up as expected, the final ring remained a snowflake, according
to the Hollywood Reporter.
malfunctions of this sort are not unusual during grand events like the
opening ceremony, the electrical problems come after multiple reports of
unfinished hotels and bad water. Officials in Sochi are likely hoping
that this is it for technical issues.
Twitter, of course, loved this.
Sochi only needs four Olympic rings. The fifth one was way gay anyway. pic.twitter.com/IhembvL8OA
— Courtney Nguyen (@FortyDeuceTwits) February 7, 2014
NBC will air the Olympics opening ceremony starting at 7:30 p.m. ET/PT Friday.


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The mission of Planned Parenthood of Southwest and Central Florida is to provide affordable access to comprehensive reproductive healthcare and accurate health information through patient care,education and advocacy.
On July 1, 2015, we completed the successful merger of three Planned Parenthood affiliates, creating an affiliate that now encompasses 22 counties in the state of Florida, serving 40,000 patients annually in 11 health centers. Overseen by committees from three Boards of Directors and exercising due legal and financial diligence, the merger led to a stronger organization that serves the reproductive health care needs of our region.
Another accomplishment was training of our health center staff for insertion of a new, lower-cost IUD for women, a birth control method that is long-acting, effective and safe. This training was provided affiliate-wide by our Nurse Practitioners.
In addition, our affiliate mobilized significant numbers of activists of all ages to communicate with elected officials and thought leaders regarding the importance of access to women's health care and prevention information.
We increased our online presence through social media to reach our patients and young people seeking information where they are – online. This includes online appointment scheduling and 24/7 access to high-quality reproductive health information.
Our goals for this year include:
-the integration of limited primary care services, to be piloted in Sarasota County, into our reproductive health care delivery system.
- an increase in development revenue by 21% overall to support the provision of services and replace Federal grants monies lost resulting in downsizing of outreach education staff and programs.
-continue providing excellent experiences for our patients and staff to increase staff retention rates and grow our patient base.
-the implementation of strategies to build the advocacy base to encourage positive legislation regarding reproductive health care and information access.
The five most pressing needs for our agency are:
1. Funding to subsidize well-woman visits and pap tests to uninsured and underinsured women. With Florida's refusal to accept funds to expand Medicaid to the uninsured under the Affordable Care Act, teens and young women remain at risk of unintended pregnancy and disease with no preventative care. $20,000 would help to subsidize well-women visits and pap tests to community members.
2. Funding for our Sarasota-based SOURCE productions program to create and disseminate a film about teens, HIV, bullying and LGBTQ inclusion. We have raised $13,500 toward our $60,000 need.
3. Funding for a an outreach educator to reach teens and young people with messages of prevention, safety, and the information to help them make good decisions, working with at-risk youth at facilities including schools, youth shelters, LGBTQ drop-in centers, in-clinic sessions, and community agencies. $50,000 would provide the educator's salary, office space, travel expenses to youth-serving agencies and educational materials.
4. Funding for public policy and advocacy needs, including outreach and visibility material, such as tablecloths, pull-up banners, signage to affix to tents, t-shirts for volunteers and staff to wear and a lightweight folding table, and funds to send young activists to Tallahassee to meet with legislators and share their concerns about reproductive health, access and prevention information issues. ($2,000).
5. Enhancement of our security equipment in our three health centers in the greater Orlando area is a priority. Our needs include the installation of additional security cameras in parking areas, entrances/exits, and front lobby and locks on interior doors that currently do not have them to provide a greater number of "safe rooms" for staff and patients ($5,000).
Planned Parenthood of Southwest and Central Florida, Inc. (PPSWCF) is an independently operated affiliate of Planned Parenthood Federation of America, the oldest and largest voluntary family planning reproductive health agency in the U.S. Planned Parenthood of Southwest and Central Florida began providing medical prevention services in the basement of Sarasota Memorial Hospital in 1966, fueled by the commitment of community volunteers who sought to provide low-cost, respectful family planning service and information to their community. On July 1, 2015, the Planned Parenthood affiliates of Greater Orlando, Collier County and Southwest and Central Florida merged to create a large regional health care provider.
PPSWCF empowers individuals to make responsible decisions about their sexual and reproductive health and fertility, by providing comprehensive and affordable health care services and education programs addressing family planning, sexual health, human sexuality and family life through eleven health centers and outreach education programs in a 22-county area. We provide high quality services with special attention paid to the accessibility of our services to underserved populations from diverse backgrounds. With affiliate headquarters in Sarasota, PPSWCF has worked for 50 years to assure access to family planning information and services for all who want and need them. Patient fees comprise 65% of our budget.
Before my senior year in high school, my father took a job in Mexico and my family, very suddenly, moved there. This experience, living in a place where the population was growing 3.5% a year, people were really poor or very, very rich, with no middle class, people going through our garbage a night, looking for food, children begging on the streets, learning that families typically had 6 children, basically runaway population growth, was an eye-opener to me. The population there was growing faster than schools could be built to educate the children, a recipe for a life of poverty for a large percentage of the population.
Providing quality women's healthcare and educating men and women about their reproductive life and their responsibilities are fundamental directives of Planned Parenthood. To deliver quality care, we must use technology for outreach and efficiency. Our organization must prepare for the health delivery systems that America must embrace in order to be competitive in the global world we now all live in.
Planned Parenthood's Reproductive Health medical program serves disadvantaged populations from diverse ethnic/racial backgrounds who typically do not have access to sexual and reproductive health care and/or education. The Reproductive Health Medical Services program provides clinical services that include reproductive health care, counseling and education, pap tests, birth control supplies, testing and treatment for cervical cancer, testing and treatment for sexually transmitted infections, including HIV,vasectomies, colposcopies, LEEPS and both medication and surgical abortion. We offer reduced fees for our services, and collaborate with area research institutions (USF, Becton Dickinson –amedical technology company that manufactures medical supplies, UCSF, and PPFA (Planned Parenthood Federation of America) for clinical research and training. Our affiliate serves over 40,000 patients in our 11 health centers, with 6,300 receiving services in Sarasota and Manatee Counties.
Our goal is to maintain affordable and accessible sexual health care in our service area. Patients accessing our medical services have the need for sexual health and family planning services and information to help prevent unintended pregnancies and sexually transmitted infections and disease. Information shared by our appointment-setting staff and health center staff results in more than 68% of our patients choosing a method of birth control during their visit. In addition, patients receive breast health screening, along with information about and testing for cervical cancer and testing and treatment for sexually transmitted infections. We know our services are effective with more than 83% of our area patients sharing that the health center staff's knowledge and customer service skills exceeded their expectations as did the quality of care and information they received during their appointment.
Our goal is to ensure that every person has access to sexual and reproductive healthcare to prevent unintended pregnancies, sexually transmitted infections and breast and cervical cancer. Our healthcare component has had remarkable success measured by the increase in patient numbers year to year, and the expansion of our service area, while keeping our health care fees contained affordable and our services accessible. As our community changes, we continue to see increases in patient from racially and ethnically diverse communities. Recent years have seen a major upswing in the rates of sexually transmitted infections with the Centers for Disease Control in Atlanta reporting that one in four young women aged 26 and younger have a sexually transmitted disease, some leading to cancer or infertility. Long term success will see a decrease in the spread of sexually transmitted infections and disease as well as a continuing decrease in teen pregnancy.
Planned Parenthood employs multiple tracing and assessments tools, including an accreditation conducted by our national organization every three years to examine and conduct quality assurance tests on medical protocols and follow up measures, yearly surveys of patients to assess patient satisfaction with quality of service, hours of operations and staff's ability to impart their knowledge in a customer service friendly fashion.
We have yearly lab and chart reviews by the state of Florida licensing division and Agency for Health Care Administration and conduct at least annual surveys with our patients to capture their assessment of our customer care, access to appropriate information to keep them safe and healthy and, hours of service and clinic expertise. We generally see patients with 48 hours of a call for an appointment and have walk-in hours daily.
In 2014-2015, our 11 health centers provided over 40,000 patients with reproductive health services, including 4,148 HIV tests; 32,855 STD tests; and 47,580 oral contraceptive/birth control refills to help prevent unintended pregnancies.
Following are letters from our patients to staff and other patients:
"I am here at PP to get tested for STDs. I am scared to death but so thankful that there are places like PP because without them I would prob {sic} never get tested. Thank you for being here for me PP."
"I just got done with my first ever Pap exam. I was completely freaked out about this but as it turned out I was worried about nothing because it didn't hurt at all (so don't freak like I did!) The nurse and staff that work here are really nice and make you feel welcome and at home. I'm definitely glad I came and will come back. Thank you Planned Parenthood for being here for me and my fellow sisters. Where would we be without you?"
Barbara Zdravecky has been Planned Parenthood's President and CEO for more than 21 years. Ms. Zdravecky has a nursing degree and has worked in the non-profit sector in various roles as a social worker, counselor and registered nurse, specifically in the fields of community mental health, and women and children's healthcare.Prior to joining PPSWCF, she held several executive positions for Manatee Memorial Hospital.She was Divisional Manager of Women's Health Services, Director of the Life Management Center and Director of Community Relations. In 2006 she was awarded the distinguished Ruth Green Award for being selected as the CEO of the year from more than 120 Planned Parenthood affiliates nationwide. She is a 1999 graduate of Leadership Florida and was appointed by Senator John McKay to the Florida Commission on the Status of Women.
Planned Parenthood collaborates with Healthy Start, Teen Pregnancy Prevention, Community AIDS Network, Safe and Drug Free Schools, and HIV/AIDS and County Health Department coalitions and task forces throughout our service area. This allows for coordination of efforts with other local agencies to avoid duplication of services and maximize our program reach throughout the communities we serve.
Indirect Public Support HelpIndirect public support represents revenue received through solicitation campaigns. This includes funding United Way and other federated fundraising organizations, but does not include donor designated contributions.
Earned Revenue HelpEarned revenue represents income generated in direct exchange for a product or service.Earned income includes income from government contracts.
Profiles in The Giving Partner do not indicate endorsement by partner foundations.
Copyright © 2015 The Giving Partner
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|[jira] Commented: (DIRSEDA-8) ListenerConfig abstraction for TCP, UDP, and intra-VM pipes.
|Thu, 16 Sep 2004 15:31:38 GMT
The following comment has been added to this issue: Author: Alex Karasulu Created: Thu, 16 Sep 2004 8:30 AM Body: Could you update your working copy, regenerate the patch, and submit again? I had a few of the patch chunks fail and after looking at the rejects it occurred to me you were working with a couple of stale files in your working copy. You might run into a conflict or two on an update however. Thanks, Alex --------------------------------------------------------------------- View this comment: http://issues.apache.org/jira/browse/DIRSEDA-8?page=comments#action_53147 --------------------------------------------------------------------- View the issue: http://issues.apache.org/jira/browse/DIRSEDA-8 Here is an overview of the issue: --------------------------------------------------------------------- Key: DIRSEDA-8 Summary: ListenerConfig abstraction for TCP, UDP, and intra-VM pipes. Type: Improvement Status: In Progress Priority: Major Project: Seda Framework Assignee: Alex Karasulu Reporter: Trustin Lee Created: Thu, 16 Sep 2004 1:49 AM Updated: Thu, 16 Sep 2004 8:30 AM Description: ListenerConfig interface is currently specific to TCP transport, and we need to generalize it and create subclasses we planned to support; SocketListenerConfig that covers TCP and UDP, and PipeListenerConfig that covers intra-VM pipes. Additionally, DefaultListenerManager is renamed to TCPListenerManager. --------------------------------------------------------------------- JIRA INFORMATION: This message is automatically generated by JIRA. If you think it was sent incorrectly contact one of the administrators: http://issues.apache.org/jira/secure/Administrators.jspa If you want more information on JIRA, or have a bug to report see: http://www.atlassian.com/software/jira
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What began as a series of articles in Men's Health magazine has become a food revolution. In five years, David Zinczenko and Matt Goulding's Eat This, Not That ! books have changed family eating habits and, in some cases, chain food menus. Based on the belief that we can find healthy alternatives to the foods we love, the series zeroes in on the restaurant and supermarket foods that we just can't resist and then offers something equally appealing with less caloric and fat baggage. A paperback that pays for itself in health.
Eat This, Not That! 2012: The No-Diet Weight Loss Solutionby David Zinczenko
With an angry food industry hot on their heels and a ravenous fan base clamoring for more, Zinczenko
and Goulding once again redefine the American food landscape.
Featuring up-to-the-minute information on the ever-changing array of supermarket and restaurant
offerings, Eat This, Not That! 2012 reveals the shocking calorie counts, mind-bending sugar/i>
With an angry food industry hot on their heels and a ravenous fan base clamoring for more, Zinczenko
and Goulding once again redefine the American food landscape.
Featuring up-to-the-minute information on the ever-changing array of supermarket and restaurant
offerings, Eat This, Not That! 2012 reveals the shocking calorie counts, mind-bending sugar and fat
loads, and deceptive advertising and marketing techniques that are making America fat—and gives
readers the information they need to fight back.
Packed with cool tips, industry secrets, and essential nutrition knowledge, Eat This, Not That! 2012
is a must-have for anyone who cares about what they eat—and how they look.
Editorial Reviews
Product Details
- 9781609610654
- Publisher:
- Rodale Press, Inc.
- Publication date:
- 09/13/2011
- Pages:
- 368
- Product dimensions:
- 6.50(w) x 6.40(h) x 0.70(d)
Meet the Author
DAVID ZINCZENKO is the editor-in-chief of Men's Health magazine and the author of New York Times bestsellers The Abs Diet, The Abs Diet for Women, and Cook This, Not That! He splits his time between New York City and Allentown, PA.
MATT GOULDING is a contributing food and nutrition editor of Men's Health and former professional chef. He lives in North Carolina.
Customer Reviews
Average Review:
Most Helpful Customer Reviews
See all customer reviews
I am glad I purchased Eat This Not That 2012 Edition. I have taken my Nook to the grocery store so I can access this book when purchasing groceries. I have lost 2.5 lbs using the book as a guide. Because of this my husband is a beleiver in the Eat This Not That books. I am going to purchase the 2012 Edition for my friends for Christmas.
Gives great insight on foods to eat at resturants as well as chosing better foods at the supermarket
I've read every book in this series. They're all extremely helpful and quite eye-opening as well. This book is a must for those who want to lose weight without denying themselves of their favorite restaurants.
I think it is well written. But it is not what I expected. I wanted a book to tell me what specific ingredients to exchange for ones that are not so good for us. This is written more for people who eat out all the time. And I rarely do that, so it wasn't as valuable to me as I would have liked. I will finish reading it to glean whatever I can that will help in my quest for better eating habits, but most of what they were exchanging, good and bad were already off my diet.
Its great!
Book seems to have useful information, but keeps locking up.
I got the 2011edition and its good but this is better. Im only 11 and this fasinates me
Love the book
I like it. Gives a lot of good ideas
I have attempted to warn consumers but Nook will not post bad reviews on this book...credibility + integrity quickly diminishing!


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Sol Elite Palmeras
Value Score
Poor Value
Costs More than similar hotelsSee Popular Hotels
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My sweet honeymoon...
I stayed a week in 1994 during my honeymoon, when the hotel was named 'Sol Palmeras'.
We spent a fabulous week, enjoying the hotel amenities, the friendly staff and the superb location.
Unique Quality: Great views of the nearby sea and beaches.
Wonderful pool.
Excellent shows at nigth (in five laguages!!)
Quiet place!!!
Veradero Hotel
Just returned from a week at Sol Elite Palmeras. Very nice hotel. Easy check in, friendly and helpful staff. Our room had a nice patio looking onto the pool and a short walk to the beautiful clean beach. Lots of choices for full meals or snacks.
We had dinner at the steak house,(not our favorite) Italian, Cuban, (my favorite) and Chinese. We were a group of 13, most of the evenings.
Buffet was plentiful with many choices.
Would certainly recommend this hotel
great allin beach hotel in varadero
The All Inclusive Resort Sol Palmeras is located alongside magnificent Varadero beach. The resort is part of the Las Américas Resort hotel complex. Right next to Varadero golf course, close to the new Plaza Americas Shopping Centre and 8 km from the town of Varadero. 35.5 km distance from Varadero airport and 140 km from Havana airport.Rooms
376 Double rooms, 28 Junior Suites and 4 Suites
200 Bungalows with one or two bedrooms
Bathroom with hair-dryer
Satellite TV
Piped music
Services and facilities
Currency exchange
Tour desk
Car and moped rental
Gift shop, Boutique, Crafts and Mini-market
Hairdresser & Beauty parlor
Laundry service
Doctor on call 24-hours
Facilities for the disabled
Car park
Unique Quality: wonderful beach, excellent food
One of Varadero's Best
I stayed at the Sol Palmeras in 2006. A great all inclusive four/five star family orientated hotel. Very clean. Wonderfully friendly staff, Great pools, incredible beach and lots of Child Friendly activities.
An all round Great Experience. Would definitely stay there again.
Unique Quality: Pool Side Bar. Three In Hotel Bars. One Buffet Restaurant and three Specialty Restaurants. Offers a Bus to and from Varadero Airport and has a very unique free Shuttle train for getting around the property and to local shopping.
All Inclusive
Sol Palmeras is a great complex. The staff are very friendly and the accommodation is good. Some fellow guests staying in the bungalows complained of insects in the bungalows, but there were none in the hotal. However the food is not that great, the buffet has a variety of food but it is cooked in an interesting way. Also it is the only restraunt that is not air conditioned. The other restraunts on site you have to book for but they do not serve the quality of food that you would expect of a similar restraunt at home.
There is a lot of poverty in Cuba and staff relay upon their tips. It is usual to give 1 or 2 pesos for a small service and to leave a 1 peso daily tip for the chambermaid.
If you have any spare clothes?shoes (even if used) or any cosmetics or shampoo (especially toothpaste or tampons) please do leave them either in your room or give them to your rep, they ensure that those in desperate need receive them.
If you 'roll your own' cigarettes take all papers, tips and tobacco with you as you cannot get them in Cuba.
Varadero hotels
It was simply called "Sol Palmeras" in 1993, so I assume it's the same hotel as Sol Elite Palmeras in the VT drop-down prompt.
Sol Palmeras is a hotel, which also has bungalows in its grounds. It borders onto a very good beach. Food was of a high standard, service was generally good. The rooms were excellent. The gardens were lush & well-maintained.
Can't remember how much it cost. I know it wasn't the cheapest in Varadero, but it was cheaper (& better value for money) than most Caribbean destinations.
Cuba, Si!
Loved it enough to return three times this year! We enjoy the bungalow because the bedroom and livingroom are separate. Enjoyed the hummingbirds hovering by the patio. Had a lovely little dog that hungout with us one trip - sorry that we didn't find her again on the others. The servers in the steakhouse are awesome - especially Raulito - what a sweetheart. The chambermaids are also very sweet - we love coming into the room to find the beautiful towel swans! Barbara even taught me how to make my own - it is a great way to let Peter know I'm ready for another trip!
Unique Quality: The beach is incredible - very clean, warm, inviting.
- 1
Nearby Sol Elite Palmeras
Nearby HotelsSee all 107
Blau Varadero Hotel 0.4 miles away
Melia Varadero 0.4 miles away
Melia Las Americas 0.8 miles away
Melia Las Antillas 0.8 miles away
Mansion Xanadu 0.9 miles away
Nearby AttractionsSee all 10
Beach 2.1 miles away
Parque Retiro Josone 3.6 miles away
Sol Elite Palmeras
We've found that other people looking for this hotel also know it by these names:
- Sol Palmeras Cuba
- Sol Palmeras Varadero Cuba
- Sol Palmeras Hotel Varadero
Address: Carretera de las Morlas, , Cuba, 42200, Caribbean
Amenities at Sol Elite Palmeras
- Restaurant
- Suites
- Swimming Pool
- Bar / Lounge
Sponsored Listings
Varadero Travel Forum
Need advice about Varadero, Cuba? Visit the Travel Forum for Varadero and ask our Answer Gurus.


text:
Walt Disney World Railroad
|Walt Disney World Railroad
The No. 2 Lilly Belle locomotive stopped at Fantasyland Station in Magic Kingdom.
|Magic Kingdom
|Opening date
|October 1, 1971
|General statistics
|Attraction type
|Railroad attraction
|Baldwin Locomotive Works
|Retlaw Enterprises
|Riders per vehicle
|375 per train
|About 20:00
|No. of tracks
|Track gauge
|3 ft (914 mm)
|Track length
|1.5 miles (2.4 km)
Closed captioning available
The Walt Disney World Railroad (WDWRR) is a 3 ft (914 mm) narrow gauge heritage railroad and attraction in the Walt Disney World Resort's Magic Kingdom theme park in Bay Lake, Florida in the United States.[1] Its route is 1.5 miles (2.4 km) in length and encircles the vast majority of the park, with stations in the Main Street, U.S.A., Frontierland, and Fantasyland sections.[2] The rail line, which was built by Retlaw Enterprises (now Walt Disney Imagineering), is operated with four historic steam locomotives originally built by Baldwin Locomotive Works.[3][4] Each of the four locomotives pulls a set of five passenger cars with seating capacity for 75 passengers per car, for a total of 375 passengers per train.[5]
The Walt Disney World Railroad opened to the public for the first time on October 1, 1971, the same day that the Magic Kingdom park first opened.[6] Since then the WDWRR has become one of the world's most popular steam-powered railroads, with 3.7 million passengers served each year.[2]
Discovery in Mexico
The development of the Walt Disney World Railroad from the late 1960s to its opening in 1971 was overseen by Mapo, Inc. (the Retlaw Enterprises research and manufacturing branch) Vice President and General Manager Roger E. Broggie, who previously supervised the building of the Disneyland Railroad in Disneyland in Anaheim, California, the sister park of the Magic Kingdom.[3][7] From his experience with the railroad at Disneyland, Broggie determined that the best option in terms of what type of steam locomotives to use would be already-existing ones, as opposed to building them entirely from scratch like the Disneyland Railroad's first two locomotives.[3] To this end, he contacted rail historian Gerald M. Best who informed him of a possible location where these types of locomotives could be obtained.[8]
The location was a railroad boneyard in Mérida, Yucatán, Mexico owned by the Ferrocarriles Unidos de Yucatán, a 3 ft (914 mm) narrow gauge system (the same gauge as the Disneyland Railroad).[9] Broggie, along with fellow Disney employee and railroad-building expert Earl Vilmer, ventured down to Mérida in 1969 to investigate and determined that four locomotives (all built by Baldwin Locomotive Works) in the boneyard, along with a fifth locomotive (built by Pittsburgh Locomotive and Car Works) in a park in front of the railroad company's headquarters across the street, could potentially be salvaged.[9]
Broggie paid a total of US$32,750 for all five locomotives (US$8,000 for each of the four locomotives in the boneyard plus an additional US$750 for the fifth locomotive in the park).[9][10] The locomotives, along with an assortment of brass fittings and other spare parts given away for free, were immediately shipped by rail around the Gulf of Mexico back to the United States.[11]
Restoration in Florida
Walt Disney World Railroad
The five locomotives and spare parts acquired by Roger Broggie, in order to receive the aesthetic and mechanical restorations necessary to run on the planned Walt Disney World Railroad, were sent to the Tampa Ship Repair & Dry Dock Company in Tampa, Florida, the closest facility to the Walt Disney World site at the time with the space and equipment needed to accommodate full-size railroad equipment.[11] Here, Transportation Superintendent Earl Vilmer, who had accompanied Broggie on his trip to Mexico, along with Project Engineer Bob Harpur and the facility's Machinist Supervisor George Britton, were tasked with the project's completion.[12]
The four locomotives built by Baldwin Locomotive Works were given brand-new, smaller boilers built by Dixon Boiler Works.[13] They were also given new fiberglass cabs as well as new tenders, which utilized the bogies from the original tenders.[10][13] Many of the smaller original parts on the locomotives such as the domes and brass bells on top of the boilers, as well as the wheels and side rods, were successfully refurbished and included in the finished products.[10][13] The locomotives' fireboxes were also reconfigured to burn diesel oil for fuel to generate steam.[13]
Despite the successful restorations of the four Baldwin locomotives, they were not able to restore the Pittsburgh Locomotive and Car Works locomotive acquired along with them.[13] It was the oldest of the five locomotives purchased and, deemed to have too many problems to be rebuilt, was stored out of use in California for a period before being sold to a locomotive broker.[13]
Opening day to present day
The four newly restored locomotives for the Walt Disney World Railroad, as well as a set of five open-air passenger cars for each of them (twenty in total) made entirely from scratch, were completed in less than two years.[5] The first of the completed locomotives arrived at the Magic Kingdom park on May 15, 1971, several months before the park's opening.[14] Like the steam trains running on the Disneyland Railroad during Disneyland's opening day on July 17, 1955, the steam trains for the WDWRR were the first attraction in the Magic Kingdom park to be finished, and they have been operating in the park ever since it first opened on October 1, 1971.[6] George Britton, who was instrumental in getting the WDWRR's locomotives refurbished, became the railroad's foreman, and he held that occupation from the time the railroad opened until his retirement on June 6, 2006.[15]
During the first few months after the WDWRR opened to the public, Main Street, U.S.A. Station was the only stop for passengers along its route, making only complete round trips possible.[16][17] That changed on May 1, 1972 when the first Frontierland Station opened.[18] It was removed in November 1990 to make way for the new Splash Mountain attraction and was replaced by the current Frontierland Station, which opened in December 1991 just north of where the original station stood.[19] The WDWRR's third station, Mickey's Birthdayland Station, opened on June 18, 1988 in the Magic Kingdom park's brand-new Mickey's Birthdayland section east of Fantasyland, and the railroad was briefly renamed Mickey's Birthdayland Express to promote it.[20][21] On February 11, 2011, after getting its name changed to Mickey's Starland in 1990, Mickey's Toyland in 1995, and Mickey's Toontown Fair in 1996, the section and its WDWRR station closed to make way for the new Storybook Circus area, which would be part of a new expansion of the Fantasyland section.[22][23] The current Fantasyland Station, built on the site of the former Mickey's Toontown Fair Station, opened in March 2012.[24]
Ride experience
Beginning at Main Street, U.S.A. Station adjacent to the Magic Kingdom park's entrance, the trains of the Walt Disney World Railroad travel along its single track in a clockwise direction on its circular route.[25] Each train is operated by an engineer and fireman in the locomotive, as well as a conductor in the back of the train who supervises the passengers.[26] After going through a tunnel through the Splash Mountain log flume attraction, the train's next stop is at Frontierland Station.[27] Continuing down the line, passengers will be able to see numerous static and animatronic displays of Native Americans and wild animals on their way to the train's next stop at Fantasyland Station.[28] In the final phase of the train's journey around the park, it passes the Space Mountain roller coaster attraction before arriving back at Main Street, U.S.A. Station, completing what the park refers to as The Grand Circle Tour.[29]
A separately-priced tour of the WDWRR named Disney's The Magic Behind Our Steam Trains Tour is available once daily, and includes access to the railroad's otherwise-restricted roundhouse where the trains are stored and maintained.[30]
Rolling stock
Each Walt Disney World Railroad locomotive when working on the line consumes 25 US gallons (95 l) of fuel and 200 US gallons (760 l) of water per hour.[6] In the past, all four steam locomotives have received overhauls at the Tweetsie Railroad in Blowing Rock, North Carolina.[31]
The table below lists the details of the WDWRR's locomotives and their passenger cars.[4][32]
|Wheel arrangement
|Date built
|Baldwin Locomotive Works serial number
|Passenger cars
|Date entered service
|Walter E. Disney
|Walt Disney
|4-6-0 (Ten-wheeler)
|May 1925
|Five red passenger cars
|October 1, 1971
|This is the tallest locomotive in the WDWRR fleet at 11 feet 11 inches (3.63 m), giving it 1 inch (0.025 m) of clearance between the top of its smokestack and the top of the roundhouse doors.[5]
|Lilly Belle
|Lillian Disney
|2-6-0 (Mogul)
|September 1928
|Five green passenger cars
|October 1, 1971
|Returned to service in 2016 after being rebuilt at the Strasburg Rail Road in Strasburg, Pennsylvania.[33]
|Roger E. Broggie
|Roger E. Broggie
|4-6-0 (Ten-wheeler)
|May 1925
|Five yellow passenger cars
|October 1, 1971
|Originally planned to be named after Roy O. Disney, but given that he did not want his name attached to the locomotive nearly identical to the No. 1 named after Walt Disney, the No. 4 was named after him instead.[32]
|Roy O. Disney
|Roy O. Disney
|4-4-0 (American)
|February 1916
|Five blue passenger cars
|December 1, 1971
|Built in 1916, this is the WDWRR's oldest locomotive and predates the Magic Kingdom's oldest purpose-built amusement attraction: the Prince Charming Regal Carrousel, originally built in 1917.[34]
See also
- Hogwarts Express (Universal Orlando Resort)
- Rail transport in Walt Disney Parks and Resorts
- TECO Line Streetcar System
- ^ "Surviving Steam Locomotives in Florida". Steamlocomotive.com. Archived from the original on February 14, 2017. Retrieved February 14, 2017.
- ^ a b "How Walt Disney's Love of Trains Changed the World". The Huffington Post. March 18, 2015. Archived from the original on March 18, 2016. Retrieved June 19, 2016.
- ^ a b c Broggie 2014, p. 317.
- ^ a b Broggie 2014, pp. 393–394.
- ^ a b c Broggie 2014, p. 331.
- ^ a b c Broggie 2014, p. 333.
- ^ Broggie 2014, p. 216.
- ^ Broggie 2014, p. 318.
- ^ a b c Broggie 2014, pp. 320–323.
- ^ a b c Amendola 2015, pp. 148–149.
- ^ a b Broggie 2014, p. 324.
- ^ Broggie 2014, p. 328.
- ^ a b c d e f Broggie 2014, p. 329.
- ^ "May 15, 1971: Walt Disney World Railroad". Orlando Sentinel. May 15, 1971. Archived from the original on June 22, 2016. Retrieved July 21, 2016.
- ^ "This is a Mickey Mouse railroad!, page two". Trains. November 20, 2009. Archived from the original on July 21, 2016. Retrieved July 21, 2016.
- ^ "Walt Disney World Railroad Main Street U.S.A. Station". TouringPlans.com. Archived from the original on June 30, 2016. Retrieved January 14, 2017.
- ^ "Main Street, U.S.A". WDWRadio. September 14, 2007. Archived from the original on January 14, 2017. Retrieved January 14, 2017.
- ^ "Walt Disney World Railroad Frontierland Station". TouringPlans.com. Archived from the original on June 30, 2016. Retrieved January 14, 2017.
- ^ "Frontierland Trivia". WDWRadio. September 14, 2007. Archived from the original on January 14, 2017. Retrieved January 14, 2017.
- ^ "Walt Disney World Railroad Fantasyland Station". TouringPlans.com. Archived from the original on June 30, 2016. Retrieved January 14, 2017.
- ^ "Mickey's Something-land…". WDWRadio. February 9, 2005. Archived from the original on January 14, 2017. Retrieved January 14, 2017.
- ^ "A look at the closing of Mickey's Toontown Fair". Attractions Magazine. February 12, 2011. Archived from the original on September 11, 2014. Retrieved January 14, 2017.
- ^ "Unwrapping Mickey's Birthdayland at Magic Kingdom". D23: The Official Disney Fan Club. June 10, 2013. Archived from the original on January 7, 2014. Retrieved January 14, 2017.
- ^ "Disney World opens first phase of Fantasyland expansion". Los Angeles Times. March 14, 2012. Archived from the original on May 6, 2016. Retrieved January 14, 2017.
- ^ "Magic Kingdom and Fun Facts". WDWRadio. September 14, 2007. Archived from the original on January 9, 2012. Retrieved June 19, 2016.
- ^ "The Quick 10: 10 Magic Kingdom Attractions and Their Secrets". Mental Floss. October 1, 2008. Archived from the original on November 1, 2015. Retrieved June 19, 2016.
- ^ Bradshaw et al. 2013, p. 233.
- ^ "Celebrate 40th birthday of Walt Disney's Magic Kingdom (and check out these 'secret' spots)". Tampa Bay Times. September 28, 2011. Archived from the original on July 31, 2013. Retrieved June 19, 2016.
- ^ "Walt Disney World Railroad". Theme Park Insider. December 12, 2013. Archived from the original on September 5, 2015. Retrieved June 19, 2016.
- ^ "Disney's The Magic Behind Our Steam Trains Tour – official website". Walt Disney Parks and Resorts. Archived from the original on March 22, 2016. Retrieved June 25, 2016.
- ^ "This is a Mickey Mouse railroad!, page four". Trains. November 20, 2009. Archived from the original on July 21, 2016. Retrieved July 21, 2016.
- ^ a b Amendola 2015, pp. 150–157.
- ^ "Surviving Steam Locomotives in Florida". Steamlocomotive.com. Archived from the original on June 28, 2016. Retrieved December 14, 2016.
- ^ "Prince Charming Regal Carrousel – official website". Walt Disney Parks and Resorts. Archived from the original on April 18, 2016. Retrieved June 19, 2016.
- Amendola, Dana (2015), All Aboard: The Wonderful World of Disney Trains (1st ed.), Disney Editions, ISBN 978-1-4231-1714-8
- Bradshaw, Kate; et al. (2013), Fodor's Walt Disney World 2013 (1st ed.), Fodor's, ISBN 978-0-307-92944-0
- Broggie, Michael (2014), Walt Disney's Railroad Story: The Small-Scale Fascination That Led to a Full-Scale Kingdom (4th ed.), The Donning Company Publishers, ISBN 978-1-57864-914-3
External links
|Wikimedia Commons has media related to Walt Disney World Railroad.
- Official website
- Geographic data related to Walt Disney World Railroad at OpenStreetMap
- Amusement rides introduced in 1971
- Amusement rides by name
- 1971 establishments in Florida
- 3 ft gauge railways in the United States
- Fantasyland
- Frontierland
- Heritage railroads in Florida
- Magic Kingdom
- Main Street, U.S.A.
- Narrow gauge railroads in Florida
- Passenger rail transportation in Florida
- Rail transport in Walt Disney Parks and Resorts
- Railroads of amusement parks in the United States
- Railway lines opened in 1971
- Transportation in Orange County, Florida
- Walt Disney Parks and Resorts attractions
- Walt Disney World transit


text:
Http proxy
leticia freitaz
Posts: 3
leticia freitaz
Posts: 3
posted 5 years ago
Then you haven't finished the code. Your code which processes the request from the browser has to get its URL from that request, not from something which came from the command line when you started the server running. And it has to return the response from the URL back to the browser, not write it to the command line.


text:
Comentarios de clientes
Corte A/Princesa Un sólo hombro Hasta el suelo Chifón Dama de honor con Volantes #007004153
Precio regular:
€ 145.91
Ahorro € 28.99
Comentarios de clientes: Deje su comentario
I bought my bridesmaid dress from this company and am extremely pleased with it. I was a little apprehensive at first but I can happily say my dress fitted perfectly and was an exact replica of the dress advertised. I would recomend you to my friends.
Im considering this dress for my bridemaids and can you please send me some pictures of this dress in blue. I need some close up pictures of this dress.
can you please send me real pictures of this dress, in burgundy if possible.
thank you
could you please send me photos of this dress in all shades of blue you carry?
I have fallen in love with this dress, but a one shoulder dress is something i have never before considered, I will be needing x 6 for my bridesmaids, i am thinking i want it itn the pearl pink but if you could kindly send me some more pics of this dress in any colours that would be big help, I would love to see it in pink but any colour you have just to see more detail would be fantastic. If you could get these over to me asap as i really need to start making a decision and getting my order in ASAP as my wedding is in July.
Kind Regards
Hi love this dress for my bridesmaids, is it possible for you to send me a picture of it in red?
Thanks Mia
Hello, I like this dress for my bridesmaids but I would only want my maid of honor in floor length, is it possible to have the hem changed to be knee length for the other girls?
Could you send me some pictures of this dress in Sage? Also, could you send me a close up picture as I cannot see the detail on this dress very well.
Many thanks
Respuesta de JJsHouse 2012-02-08 00:29:49
Hi Amy,
Thank you for contacting us.
Please check the attachment to see the pictures of it in Sage. Hope they would be helpful to you.
If you have any other questions, please feel free to contact us.
hi could u send me a picture of this dress in gold ivory and brown please
Respuesta de JJsHouse 2012-02-04 21:58:26
Hi Isla,
Thank you for contacting us.
I have attached some pictures of it in Gold and Brown for your reference as pictures of it in Ivory are not available here. Hope these pictures would be helpful to you.
If you have any other questions, please feel free to contact us.
Can you make this dress in a clover colour?
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text:
Safety Charles Woodson would obviously love the Raiders to add DeSean Jackson.
Charles Woodson on Desean Jackson: "A guy as talented as he is, when u have that opportunity to bring him in, I think you take a shot at it"
— 95.7 The GAME (@957thegame) March 28, 2014
Jackson is from the area and the Raiders have the funds to offer him an appropriate contract. If the Raiders can get past Jackson's off the field issues then it is very possible.