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Custom Project Jan 03 2013 US Fans
- Status Closed
- Budget $10 USD / hour
- Total Bids 1
Project Description
hello, I need 1000 fans added to a Facebook fan page. All fans must be from US ONLY. I do NOT want: - fans from other countries being mixed in - fans with pornographic or sexual images being added All fans must be real accounts and ..must be active Facebook users .This is a test job to find a new supplier for large company. This job must be completed in 3 hours during one week . If not your bid will be down graded. I need more suppliers to handle our work load. The money will be paid 2 weeks after job is complete and all the fans are still on the Facebook page
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According to me, escort service is not a mere business but it is a profession that is purely close to glamour world. I mix my own passion and whole lot of sensation to make my clients satisfied and happy. Hence my clients get awesome results after spending good times with me. I am very much aware of my beauty and know how important it is to blend in class and culture with it. The elite clients who get accompanied by me, finds the whole interesting and memorable, because the class and sophistication they look for in their women companions, get actually in me.
Excitement gets doubled when men find lovely and sexy women companions by their sides in their holiday trips. Obviously I am a mature and professional escort but the way I accompany the clients during their holidays is just mind blowing in a word. My usual young self get wings while holidaying with fun loving clients. As an important part of outcall service, I accompany clients in short weekend trips, long vacations in regional and foreign locations. Those who love to have the pleasure of enjoying a romantic long drive with me can also have their share of fun and romance by getting in touch with me. I assure premium quality services to all my clients if they are willing to spend their holidays with me.
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subset: commoncrawl


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Slow PunctureLast updated at 12:01AM, March 26 2016
The government is neglecting its promises to cyclists
A descendant of Queen Victoria has admitted hitting a cyclist and driving off without stopping while riding his Harley-Davidson past Hyde Park Corner.
Nicholas Brandram, whose grandmother was Princess Katherine of Greece and Denmark — the last surviving great-granddaughter of Queen Victoria — struck Martha Franklin's bicycle on March 6.
Brandram, 31, told City of London Magistrates' Court that the roar of his 1948 Harley-Davidson might be the reason he did not recall any impact, but he questioned how Ms Franklin had managed to stay on her bike.
The decorated former British Army captain, who has served in Iraq, Bosnia, and Afghanistan, admitted crashing into Ms Franklin and driving off.
Brandram, a stockbroker who represented himself, said that as a cyclist he was aware of the risks involved and that he took "full responsibility" for any damage to Ms Franklin's bicycle.
"On March 6, Mr Brandram was driving his Harley Davidson motorcycle on the Hyde Park Corner when he collided with a cyclist," Kerry McNulty, prosecuting, said.
"Her back wheel was damaged and I have no injuries caused. Mr Brandram then left the scene without exchanging details."
Brandram told the court he drove away from the scene because he was unaware he had collided with Ms Franklin.
"I remember an incident while on my motorbike in the area described by the prosecutor and I remember a big group of cyclists in traffic," he said.
"I had right of way as the zebra crossing was 60 metres away — I kept going when one of the cyclists came ridiculously close to me.
"The claim is that I damaged their back wheel but I don't understand how they wouldn't have fallen off and me as well.
"I provided first aid before to someone who has had an epileptic fit so I would have stopped if I'd have known. If I've caused damage I take full responsibility for that."
Brandram's 1200cc Harley Davidson is large enough to knock a cyclist over at five miles an hour, the court was told.
He added: "I have a very loud bike so it's not impossible I collided with her without me realising it. At the same time, with such a big bike, I don't know how I didn't feel the collision."
Brandram, from Fulham, West London, pleaded guilty to driving without due care and attention and failing to stop after a road accident. He was given five penalty points, a £200 fine and told to pay £105 compensation.
The government is neglecting its promises to cyclists
The cycling and walking investment strategy pledges £300 million over five years, which is less than 14 per cent of the recommended amount
A minister labelled cyclists "the biggest challenge for a commuter", as the number found guilty of neglect of traffic directions more than doubled
Mary Bowers, 31, has severe brain damage after Petre Beiu, a driver for Lynch Haulage, failed to see her waiting on her bike in front of his truck
RBS 6 Nations stars on how they come together as a team to succeed
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- Mechanical Keyboard
- Cherry MX Blue Switches
- 104 Normal Keys
Rosewill MEchanical Keyboard 02/15/2013
This review is from: Rosewill RK-9000I - Mechanical Keyboard with Cherry MX Blue Switches
Excellent Quality. If you love the tactile for touch typing, this is the keyboard for you. Comes with 2 cables for use with older PS2 interface keyboards.
Did you find this review helpful? Yes / No Thanks for the valuable feedback you provided!


text: In an analysis of data from the Nurses' Health Study and the Health Professionals Follow-up Study, researchers found that individuals who ate nuts at least five times a week were 29% and 11% less likely than those who did not eat nuts to die from heart disease or cancer, respectively. Eating nuts regularly was linked to a lower risk of dying from any cause in the 30 years studied. The findings appear in the New England Journal of Medicine.


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This is a photo of an arilbred seedling of mine. Its parentage is (King
Solomon's Mines X Welcome Reward). It is a 1/2 aril arilbred (OGB).
A short while after opening, the line coming from the bottom of the signal
darkens and reaches the tip of the falls. To me, it is a bewitching
seedling in its coloration, and I have just filled out a registration form to
name it and register it with AIS. It is fertile both ways, as is the (One
More Kiss X Sheik) seedling I posted a photo of earlier tonight.
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4 results found
Carne now has twenty-seven of the most dramatic, natural duneland holes in the world."(Robin Hiseman, European Golf Design, 2013)
Read more
Westport will leave great memories of a unique course on the shores of Clew Bay. The 11th, with its Everest like fairway, the par three 12th and the index one, par four 13th, is the Amen Corner.
Read more
The 40 shades of green visible from the clubhouse greatly enhance the scenic aspect of the course, where the lush fairways, carefully manicured greens and strategically placed water hazards and bunkers all add variety to the golfing challenge presented.
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Golfmayo is Mayo's leading corporate and holiday golfing specialists.
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The beginning of summer makes me thirsty.
Well, more like the hot weather in the South drives me to drinking…
at least to always have a cold beverage within arm's length.
I've admitted it before that I'm a drinker. I LOVE drinks. Not necessarily the spiked kind, but definitely most everything else.
And so when summer rolls around I've got to have a cold glass of lemonade. (Or sweet tea, or iced coffee, or a strawberry and banana smoothie… whatever floats my boat at the moment.)
Last summer I was testing out a new combination and love, love, loved the addition of lavender to my regular ol' lemonade. And I'll share my secret to making this the easy way!
Lavender Lemonade Ingredients:
1. 1 cup of sugar
2. 2.5 cups of water
3. 1 cup of freshly squeezed lemon juice (I used 9 medium lemons)
4. 2-3 drops of Lavender essential oil (quality matters, so I would only recommend using therapeutic-grade essential oils that are safe for ingestion. I personally only use Young Living.)
5. 3-4 more cups of water
6. (optional: 1/4 cups of lavender buds to replace the lavender essential oil.)
1. Heat the sugar and 2.5 cups of water over low-medium heat until sugar is dissolved.
2. Remove syrup from heat and let cool for about 15-20 minutes, then add the lavender essential oil to taste. I used 3 drops, but if you want a more subtle taste, try 2 drops. You can always add more, but can't take it out. 🙂
3. Pour into a large jug and top with lemon juice and the remaining water. I use 4 more cups of water, but add to your personal taste.
4. Chill until ready to drink
5. Optional: If you want to use lavender buds, you can use them in place of the essential oils. Just add 1/4 cup of lavender buds to the water/sugar mixture in step 2. Let it steep for 1 hour, then strain out the buds and finish with steps 3 and 4.
Yields ~2 quarts.
Note: Lavender and Lemon are two of my very favorite essential oils. Come find out how I use them:
14 Amazing uses of Lavender Essential Oil
12 Amazing uses of Lemon Essential Oil
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This post is sponsored by my YL. I love having natural options and these essential oils have helped my family tremendously in our pursuit of natural wellness. They are also my business and are so well-loved that they sponsor my blog!! Enroll by clicking here.
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100 Ideas for Teaching Personal, Social and Emotional Development
By (author) Judith Thwaites
Format: Paperback
Normal Price: $26.99
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100 Ideas for Teaching Personal, Social and Emotional Development by Judith Thwaites
Book DescriptionThis book provides a practical, dip-in resource to help early years practitioners meet the requirements of the Personal, Social and Emotional area of the EYFS framework.Every Early Years practitioner, including childminders, will have a statutory duty to deliver the new Early Years Framework. This book provides a practical, dip-in resource to help them meet the requirements of the Personal, Social and Emotional area of the framework.This series offers 100 practical and innovative ideas for all practitioners working with children in the early years.
Buy 100 Ideas for Teaching Personal, Social and Emotional Development book by Judith Thwaites from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9781847063823
(244mm x 169mm x 10mm)
Pages: 128
Imprint: Continuum International Publishing Group Ltd.
Publisher: Bloomsbury Publishing PLC
Publish Date: 10-Oct-2008
Country of Publication: United Kingdom
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Author Biography - Judith Thwaites
Judith Thwaites has worked in Education for over 30 years. Having taught at Foundation Stage and Key Stage 1 she is concerned with devising strategies for delivering the EYFS curriculum in stimulating and meaningful ways.
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Colonels to open '12 season in Corvallis
THIBODAUX — The Nicholls State University football team will open the 2012 football season in Corvallis, Ore., against the Oregon State Beavers of the Pac-12, Nicholls athletics director Rob Bernardi announced today.
The opener at OSU will mark the first time a Colonel football team will play a Pac-12 (formerly Pac-10) opponent.
"Part of what I want to do here at Nicholls is give our student-athletes a great experience," said Nicholls football head coach Charlie Stubbs. "I had the pleasure of coaching at Oregon State for six years. This is now a wonderful opportunity for our student-athletes to compete against a Pac-12 school and see the beautiful Pacific Northwest. Beavers head coach Mike Riley runs a first-class operation and we're very much looking forward to the game."
Oregon State is one of three FBS opponents on the Colonels' 2012 schedule. Nicholls is slated to play Tulsa and South Alabama in non-conference contests as well.
"As we're building our program, we need to play in these types of games," said Coach Stubbs. "We're bringing in competitive student-athletes who are eager to see to how they stack up against high-level competition."
The Colonels open the 2011 season on Thursday, Sept. 1 at 6:30 p.m. against Evangel University at John L. Guidry Sta-dium in Thibodaux.
Tickets are available by calling (985) 448-4790 or by logging on to ticketmaster.com.
Nicholls will host its Colonel Kickoff Luncheon at 12 p.m. this coming Monday at Envie Restaurant and Oyster Bar in Thibodaux. The cost to attend is $15. More information is available at www.geauxcolonels.com.
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Christian Classics
Treat yourself or someone you love to one of these Christian classics from Family Christian, celebrations of faith that never go out of style. These classic Christian books always make great gifts! And like a good friend, they are always there when you need them most.
In Everything by Prayer
A. W. Tozer, W. L. Seaver$14.99Add Your ReviewIn Everything By Prayer combines the best of Tozer on prayer into one volume. Tozer was captured by God s glory, and he regarded prayer as the primary means of coming into His presence. But if our days are filled with the barrenness of busyness and there is no serious urgenc... Learn More
Andrew Murray, Donna Partow, Irene Hannon, Andrew Murray$7.99Add Your ReviewThe Journey Toward HolinessWhen Jesus "made himself nothing...taking the nature of a servant," He modeled for all believers true humility. Andrew Murray calls this "our true nobility" and "the distinguishing feature of discipleship." With insightful, penetrating clarity, Mur... Learn More


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It seems there are endless lists of "best employers", "most engaged", "most diverse"… but we all know that with the good there must be bad. These bad are rarely in the spotlight, unless it's due to a court case or a high profile resignation.
Websites such as Glassdoor allow employees to rate companies on a range of factors, now 24/7 Wall Street has used that data to examine publicly traded companies with more than 300 reviews to track the low-rated US companies – and look at the reasons behind those numbers.
10. GameStop
The common complaints here are similar to those heard from many retail workers – too much emphasis on making the sale, even at the detriment of the customer. For every Zappos sending free shoes to an inept groomsman there's a manager telling staff that sales matter more than anything else.
"Priority is placed on sales instead of games and customers, pushing people to pre-order games can place them in a situation where they spend good money on a bad game with no possibility of a refund, business models place customers at a disadvantage," read one review. The attitude isn't doing the company any favours – GameStop made Consumer Report's annual "naughty" list for bad customer service last year.
9. Rite Aid
The drugstore chain settled an overtime lawsuit for almost $7 million in 2009, but it doesn't look like they've been able to change their staff's attitude. Reviewers repeatedly suggested that managers did not know what they were doing because they are not "given clear directions on what they should be doing." Reviewers also consistently objected to "mandatory overtime" and "working holidays."
8. Hewlett-Packard
After going through two CEOs in as many years – one leaving after a scandalous relationship with a contractor – Hewlett-Packard Co. could perhaps be forgiven for some management turmoil. Unfortunately it seems its inconsistency has affected staff, who complain about the company's poor performance and management failings. However, there could be good news on the horizon as Glassdoor's CEO rating for Meg Whitman, appointed in 2011, is high despite a recent announcement that HP would cut 27,000 jobs – which reviewers say will not solve the company's problems.
7. Robert Half International
It could be the industry that Robert Half International Inc. works in that is affecting its grade. As a provider of temporary workers their turnover is understandably high so these out-going workers could sway results.
The main complaint from reviewers was that the amount temps are paid is undercut by the amount Robert Half takes out of each paycheck. "Pay is below what you can earn in similar sales roles, considering how much you are charging your clients. They want to make a huge margin making it impossible to be competitive with pay for placements." A number of reviewers also said that the company's focus on "activity metrics" and "growth expectations" over "team morale" created a "hostile work environment."
6. Sears Holdings (Sears/Kmart)
The international department store's "ancient systems" are in desperate need of repair, according to employees, who are unhappy with compensation. Sears employees consistently pointed to low starting salary and even lower annual raises. Kmart employees complained they cannot get enough pay as they are limited to fewer than 32 hours a week with shifts only "four to six hours long." In 2011, Sears' American Customer Satisfaction Index score was a 76 out of 100. Among all department stores and discount retailers, only Walmart received a lower score.
Find out the top five here: The worst companies to work for: Part 2
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Channel sultry, sixties allure with these on-point Cat Eye Sunglasses. A style that flatters all, these chic sunnies have been finished with tinted and gradient pink lenses for cool and contemporary effect.
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text: From Deep in the Central Valley: The Tercelamino!Davey G. Johnson3/21/06 2:00pmFiled to: Custom Cars/Hot RodsEl CaminoToyota0EditPromoteShare to KinjaToggle Conversation toolsGo to permalink This image was lost some time after publication. Our man Damon from Drivewire happened by this rare specimen of Caminage at his local DMV. A 1980s Toyota Tercel 4WD wagon converted to keg-haulin', Semper Fidelisin' duty on the backroads of the grand San Joaquin Valley, the customizer ingeniously used the rear tailgate as the back window of the pickup cab and (we're assuming), welded the rear doors shut a-la the General Lee. Can you say "meth runner"? We knew you could. Advertisement More El Caminos [Internal]ReplyLeave a reply


text:
This shirt was made as a thank you to our rad pastor, Perry Noble. It's Pastor Appreciation Month and I created this shirt design of the saying "Hold Onto Your Fork, The Best Is Yet To Come" that Perry often says & Ephesians 3:20. I also created a thank you card for his awesome leadership of this church!
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USGTF Certification Courses are currently being offered for all Korean-American citizens that want to become qualified golf teaching professionals but lack proficient English to gain their certification. If you are US-Resident and wish to take the course in the Korean Language – please click here. These courses are conducted by the same standards and costs as the USGTF (English) courses.
The following schedule of Certification Courses are for Korean-American Citizens only and are conducted by Mr. Joseph Lee (Master Golf Teaching Professional and Certified Examiner) in the Korean language only:
Certified Golf Teaching Professional® courses:
|April 13-17, 2016
|Bear's Best GC
|Suwanee, GA
|May 11-15, 2016
|Needwood GC
|Derwood, MD
|May 25-29, 2016
|Wilson GC
|Los Angeles, CA
|June 22-26, 2016
|Seattle, WA
|July 13-17, 2016
|Brunswick, NJ
|August 3-7, 2016
|Westridge GC
|Fullerton, CA
|September 8-11, 2016
|Battle Ground GC
|Deer Park, TX
|October 5-9, 2016
|Lakewood GC
|Lakewood, CA
|November 2-6, 2016
|Wilson GC
|Los Angeles, CA
Master Golf Teaching Professional® courses:
|May 16-19, 2016
|Needwood GC
|Derwood, MD
|November 6-9, 2016
|Wilson GC
|Los Angeles, CA
For more information on these courses or to register, please contact: Joseph Lee (Master Golf Teaching Professional / USGTF Examiner) at (714) 313-6447 / firstname.lastname@example.com


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Listen to free music by Victorious Cast
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shef7 Ok, my friend liked this song... its okayy
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Excellent hotel. Awesome rooms and service in a great location. Popular among people traveling with friends.
Guest Review Highlights
- Great location
- Great room
- Rather comfortable
- Fantastic service
- Friendly service
- Great reception
Wego searches multiple travel sites so you can find and compare the best rates.
Based on 26 reviews from across the web.
Excellent hotel. Awesome rooms and service in a great location. Popular among people traveling with friends.
Check-in: 14:00
Check-out: 12:00
All the team of MY HOUSE TRAVEL is happy to satisfy your requests to make your stay in LUXURY APARTMENTS more pleasant and comfortable and let you feel the history of Prague that spans over thousands of years. Today Prague center is a mosaic of old houses, churches, towers and bridges. You will definitely feel the ambience of the Medieval Prague while exploring the maze of narrow streets.My House Apartments are in the Prague Jewish quarterSome of our luxury apartments are located in the famous Prague Jewish quarter. You will be only minutes away from ancient synagogues, Old Town square, Charles Bridge, Wenceslas square, National Theater and other major attractions. Here you can feel the spirit of ancient centuries and feel the true soul of wonderful Prague.Prague center is full of history and magic. Old buildings look magnificent any time of day. Location of our luxury apartments will let you enjoy Prague center any moment convenient for you - at sunrise, during the day or at night with My House Travel.


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Can You Can Money In Virtual World? – SecondLife.com Review
Second Life (SecondLife.com) is a virtual world with the population of almost 2 million people. Here you can create your avatar (the three dimensional character) and be able to meet and chat with other people (who play this game on the other end). Here, everything can be done, it's a virtual world (some people say that they hold online meetings with Second Life). Using this website you can buy, sell or rent land and also you can personify your avatar by creating your own clothes, working spaceship, or apartment.
If it's a virtual world, there are money to be made too, right? Yes, but how do you make money with SecondLife.com?
Before I start telling you about earning money with Second Life, I need to tell you what are the basic necessities.
The first thing you need to get started – fast internet connection and powerful computer with a awesome graphics card. It's pretty much the same as any other Internet game. SecondLife is a three dimensional world and the graphics are sweet.
The next step is to sign up at SecondLife.com. You can register free basic account, which can be upgraded for 10$ a month, once to get really good with the game.
Now more about the local currency – Linden Dollars
Every person in this virtual world gets weekly salary, which is about few hundred Linden dollars ($L). Virtual money can be converted to actual money and the exchange rate is about 250$L to 1$
In addition, you can work for someone or start your own business to earn Linden dollars. So people has become PROs in this game and are earning their full time income online in virtual world.
How can one make good money with Second Life?
Design and Construction Businesses
If you have some programming 3d modeling or photoshop skills then you can start making some good money here.
Let me give you some ideas –
3d modeling
Depending on your creativity you can create anything to sell in this virtual world (anything from custom bodies to vehicles, plants, homes to commercial buildings). It can be done to anything, if it does look good. Anything can be sold.
Here you have a lot of options too. You can creat custom textures for anything from clothing design to marble flooring can earn you good money. People over there want to look good and would pay you to make them look fabulous.
There are still more ways to earn with this website, the potential is just huge if you have some skills. But you never know what your skills are, so you can try out your luck!
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About The Author
For almost 10 years the IncomeBooster team has been looking for extra income opportunities, reviewing them and sharing with the community in order to make the dream of working in the comfort of one's own home, while enjoying flexible schedule, come true.


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Doctor Data Recovery
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Man Allegedly Caught Sneaking Guns into BU Graduation
A 28-year-old Marshfield man is in police custody after he allegedly tried to bring two handguns into the Boston University commencement ceremony Sunday.
Andrea Massa was arrested by Boston University Police at a nearby security checkpoint around 1 p.m., Metro Boston reports.
Boston University spokesperson Colin Riley told Boston.com the suspect "didn't want to let police stop him," which prompted additional scrutiny.
Riley said Massa is not a student at the school.
The campus was under heightened security for the event, which included commencement speaker Gov. Deval Patrick.
Massa will be arraigned in Brighton District Court Monday, facing charges of disorderly conduct, carrying a firearm on school grounds, and violating the terms of his license to carry for hunting and target practice, which is a civil offense, according to the Boston Globe.
WHDH-TV News reporter Adam Harding reportedly spoke to Massa's wife, who called the incindent a "misunderstanding."


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Scullers Jazz Club
Saturday, Jan 26, 2013 8:00 PM EST
Jose James
Saturday, Jan 26, 2013 8:00 PM EST
Scullers Jazz Club, Boston, MA
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"No Beginning No End" CD Release!
James has already established himself as a trailblazer for his intoxicating blend of jazz, hip-hop, R&B and electronica from his previous three albums.


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Jan. 3, 2013, Tucson, AZ — The January Tomato and Lettuce Intensive Short Courses (Jan. 2 – 9) of the University of Arizona/Controlled Environment Agriculture Center have been successfully filled to capacity, and are now in progress!
That's good news for the program, but not so for those with interest and need, but who could not attend.
UA/CEAC has created this Special Free Opportunity, and invite growers to view selected presentations on Jan. 7.
The only "cost" is your time. This opportunity will bring to you presentations by our experts in greenhouse crop production, including:
• Dr. Patricia (Pat) Rorabaugh and Myles Lewis
1:00 – 2:30 p.m. [Tucson Time – Mountain Time]
Environmental Control Requirements for Greenhouse Tomato and Lettuce Production — Set-points (for tomato and lettuce); how the environment affects plants (and vice versa); real-time monitoring (example, CEAC – "Tomatoes Live2!"); and deciphering your greenhouse (example, USDA "Virtual Grower.")
• Dr. Merle Jensen
7:00 – 8:00 p.m. [Tucson Time]
Hydroponic Growing Systems and the Crops They Support: Today and Tomorrow — Structures, plant environments and nutrient solution delivery.
Click on this link at the times indicated above, and join the presentation. You will have access to the audio and video of the presentations. And, you could send an email to
with your questions during the presentations, and we will respond during the Q&A period.
This seminar will be web cast at http://cals.arizona.edu/ecat/webcasts/ceac
This link provides help and information on downloading Media Player for PCs and MACs: http://cals.arizona.edu/ecat/webcasts/webcast_help.htm.
Questions from online viewers can be emailed to
either before or during the webcast.
The next Short Course is planned for April 7–12. Follow the planning at http://ag.arizona.edu/ceac.
Selected UA-CEAC short course sessions online
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Student Choice is committed to providing tools that help families better understand the financial aid process. As part of that commitment, we offer a quarterly webinar series, led by our College Access Counselor. Check out and register for our next topic "Deciphering Award Letters".
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Best Dishes at Burger King
These dishes from the Burger King menu are contributed by Menuism users directly, as part of a restaurant review, or as part of an image upload.
breakfast burrito, daily, whopper lunch, dinner ()
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This email was created by [MemberName] <[MemberEmail]> who thought you would be interested in the following Article:
"Exploring Client Callback" Client Callback is one of the very important features provided by ASP.NET. Earlier I was not aware on this. In my last application, I implemented this and got a very good result. This is another way to avoid full post back on the page and can be a great alternative of the Ajax update panel. Also [..
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text:
|Publication number
|Publication type
|Application number
|Publication date
|Aug 22, 2000
|Filing date
|Jul 3, 1998
|Priority date
|Jul 11, 1997
|Fee status
|Also published as
|Publication number
|Paul Thomas Coughlin, George Frederick Lyman, Gregory Mathus
|Original Assignee
|Corning Incorporated
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (18), Non-Patent Citations (1), Referenced by (62), Classifications (10), Legal Events (5)
|External Links: USPTO, USPTO Assignment, Espacenet
This application claims the benefit of the priority filing date of U.S. Provisional Application No. 60/052,311, filed Jul. 11, 1997.
The invention relates to a product for the culture of cells.
Conventionally, cells have been grown attached to glass or plastic roller bottles and flasks. This approach does not lend itself to high density growth of cells or continuous cell culture, and requires large amounts of medium and space. Further, the approach is labor intensive.
To achieve higher density growth conditions, various attempts have been made to use arrangements of stacked plates, the surfaces of the stacked plates providing increased surface area for cell attachment and growth. In spite of various attempts, the cell culturing devices of the prior art all have various drawbacks, including the need for excessive amounts of medium, the inability to provide a continuous flow of nutrients to all cell-growth surfaces, the need for labor intensive monitoring and care of the growing cells and the inability to operate continuously.
One attempt to solve the problems of the prior art is disclosed in commonly assigned U.S. Pat. 5,240,854. This patent discloses a cell culture system for large scale production of cells and cell products. While this culture system solves many of the problems of the prior art, a demand exists for a similar device for small scale production. The advantage of having a cell culture system of small scale, such as the present invention, is twofold. First, the cost of a smaller system would enable smaller scale facilities to harvest cells with minimal effort and thereby save the expense of purchasing a larger scale unit. Second, a smaller unit would enable users to test and perfect procedures and growth conditions for a particular application, in the laboratory, and prior to transferring to a large scale production level system.
It is therefore an objective of the present invention to provide a cell culture device having the following characteristics: high cell growth density compared to the amount of nutrient medium in the system; an even flow of medium over a plurality of growth chambers; automatic and continuous addition of nutrient medium and removal of medium containing waste; a self contained media oxygenator; a self contained media reservoir; a pre-plumbed system having a minimum of sterile connections; a continuously monitored cellular environment; the ability to harvest cell products such as biochemicals, vaccine virus, and pharmaceuticals; cost effectiveness; and usefulness for small scale experimental pilot projects.
FIG. 1 is an exploded view of the cell growth system of the present invention.
FIG. 2 is a three dimensional view of the cell growth system of the present invention.
FIG. 3A is a three dimensional view of a cartridge of stacked plates that, together with the cell growth tank, form the cell growth chambers of the present invention.
FIG. 3B is a three dimensional expanded view of a portion of a plate of the present invention.
FIG. 3C is a bisection of FIG. 3B.
FIG. 3D is a cross-section of a plate of the present invention.
FIG. 4 is a three dimensional view of a one piece combination construction of a reservoir tank lid and cell growth tank of the present invention.
FIGS. 5A and 5B are three dimensional views of the cell growth tank lid of the present invention.
FIG. 6 is a three dimensional view of the reservoir tank of the present invention.
FIG. 7 is a schematic drawing illustrating the operation of the present invention.
FIG. 8 is a cross-sectional view of one embodiment of an oxygenation device of the present invention.
FIG. 9 is a cross-sectional view of the present invention taken along the section line A--A of FIG. 1.
FIG. 10 is a cross-sectional view of the present invention taken along the section line B--B of FIG. 1.
The cell culture device of the preferred embodiment includes two separate tanks, one smaller cell growth tank seated within a larger reservoir tank holding cell growth media. Ideally, the media will circulate from the reservoir tank through the cell growth tank, and back to the reservoir tank.
FIG. 1 shows an exploded view of the preferred embodiment of the present invention. The reservoir tank 12 is a substantially rectangular box having roughly a square horizontal cross-section. The inner cell growth tank 14 and attached reservoir tank lid 16 fixably insert within the reservoir tank 12. The reservoir tank lid 16 has projecting from it, a series of nozzles or fittings 18 capable of attachment to hoses, and a container neck 20 capable of receiving a screw-on cap 22. Below the lid, and in fluid communication with certain nozzles, are a series of tubes 24 that descend downward into the reservoir tank 12. As will be discussed later, the tubes descend to varying depths within the reservoir tank. A cartridge of stacked plates 26 fixably mounted together, fits slideably into the cell growth tank 14. The individual plates are preferably all shaped identically, and the cartridge as a whole preferably conforms roughly to the interior dimensions of the cell growth tank 14. A lid 28 having a penthouse roof shape covers the cell growth tank 14. The lid 28 has an outlet conduit 30 in the form of a nozzle located at its peak. The nozzle is capable of attachment to a hose.
Once assembled, as shown in FIG. 2, the cell culture device 11 appears as a unit having four vertical side walls 32, a top surface 34, and a bottom 36. The top surface comprises the lid 16 covering the reservoir tank and the lid 28 covering the cell growth tank. From the top surface, a plurality of air and liquid tight fittings 18 protrude upwardly. A screw-on cap 22, covers a neck providing access to the reservoir tank. As an alternative to a standard cap, a vent cap, with integral sterility filter can be used to help vent the system.
FIG. 3 A shows a plurality or cartridge 26 of plates that, together with the side walls of the growth tank, form the growth surface for cells. The space between each stacked plate defines individual growth chambers. Cell growth may occur on the plates forming both sides of this chamber. Preferably, 15 plates are spaced 0.04 inches apart or greater. Of course, the system is scaleable and the number of plates may be increased or decreased accordingly. Although the plates may be spaced closer to one another, air bubbles tend to become trapped between the plates and interrupt the even flow of medium when spaced less than about 0.04 inches apart. The surfaces of the plates may be roughened, corrugated, convoluted or otherwise irregular to increase their surface area and the number of cells capable of growing on a given plate. If irregular, the 0.04 inch spacing between the plates should be between the facing peaks of irregular surfaces. The surfaces of plates may also be surface treated in a variety of different ways in order to promote cell growth. Typical treatments include carboxyl group treatments, collagen treatments, plasma treatments, fibronectin treatments, or feeder cell layers.
The plates according to the preferred embodiment are molded from polystyrene. Suitable plate materials include styrenic materials, polyester, polycarbonate, polymethyl pentene or K-resin. However, the plate may be made of virtually any material that is sufficiently strong, nontoxic, biocompatible, and otherwise suitable for cell tissue culture.
The plates of the present invention have a construction that facilitates flow distribution and manufacturing. Preferably, each of the plates are designed with at least one 0.04 inch high rib 40 or bead on their surface. The six raised pins 42 on the sides of the plate are also preferably 0.04 inches high, so that when the plates are stacked, the open area between plates is uniformly 0.04 inches thick. The pins 42 interlock with corresponding pins of successive plates and thereby serve to hold the stack of plates together while also preventing warpage of individual plates.
Plates can be made without the centralized rib portion for instances in which a confluent layer of cells is desired for harvest, for skin transplants, as an example. In these instances, the pins may serve as the only means for maintaining the required separation between plates.
Further, the plates are preferably rectangular in shape with a tapered section 44 narrowing to a double triangle point 46. As shown in FIG. 3B, a three dimensional partial view of a plate 29, each triangle is molded onto one side of a plate surface and raised 0.04 inches above the plate surface. A passage 45 extends between the triangle shaped features to the interior space defined by the side walls of the stacked plates. Each passage has side walls defined by chamfered corners 41 of the two raised triangular features 46, the plate surface, and the flat under surface of an opposing stacked plate. The extreme end of the plate is a beveled edge 39 which serves to break up and reduce the size of any bubbles that may enter through the passage 45. FIG. 3C shows a section cut of the plate 29, bisecting the passage 45 of FIG. 3B. The beveled entry 39 to the chamber can be seen clearly. The particular shape of the passage in the preferred embodiment includes a narrowed internal portion having a width of 0.04 inches. The cross-section of the passage then increases continuously and in a direction toward the growth chamber. The passage may be shaped in any of a variety of ways, including straight walled, walls flaring in, walls flaring out, or walls narrowed in the center, for example.
It is preferred that the plates fit into the growth tank such that, taken as a whole, they roughly conform to the shape of the tank. For this reason, it is preferred that the plates increase in thickness from one end to the other as shown in the exaggerated plate cross section of FIG. 3D. Pins 42 are shown on both sides of the plate 29. These pins will interlock with pins from successive plates. The pins 42 and the triangular element 46 are all raised 0.04 inches above the surface of the plate. This way, the chambers that are formed by the plates will remain a uniform 0.04 inches in width, while the plates, taken as a whole will conform to the slight draft angle of the side walls on the cell growth tank. Maintaining a uniform chamber width facilitates a smooth and even flow of media through the cell growth tank. The edges of the plates are also preferably molded with a draft that conforms with the draft on the end walls of the growth tank.
FIG. 4 shows the growth tank 14 of the present invention. The growth tank is a self contained unit that fits inside the reservoir tank. The growth tank 14 comprises two opposing side walls 50 and two opposing end walls 52 together defining an open top 56. The bottom wall 58 includes two descending ramp sections 60 that converge in a rounded trough 62. A nozzle that serves as an inlet conduit 64 for the media, extends from the center of the trough 62 and is in fluid communication with the trough. The tank 14 is preferably an injection molded thermoplastic material and thereby has a slight draft to its side walls 50 and end walls 52 to enable ejection from a mold cavity. Ideally, when inserted within the reservoir tank, one side wall 50 from the cell growth tank 14, along its entire surface, will contact a side wall from the reservoir tank. The open top section 54 of the tank is skirted by a lid 16 that will fittingly engage the reservoir tank. The cell growth tank is shaped to hold a cartridge of attached plates. The outermost plates of the cartridge and the interior opposing side walls of the growth tank form additional chambers for cell growth. The spacing between the side walls and the cartridge is preferably 0.04 inches.
FIGS. 5A and 5B show a lid 28 that engages the open top 54 of the cell growth tank 14. The lid 28 is preferably removable so the user may remove the cartridge of plates held therein and harvest the cells that have grown on the plates. Further, the lid 28 peaks in a penthouse roof leading to a nozzle which is an outlet conduit 30 for the tank. The lid 28 takes this shape so that any air bubbles contained within the tank will ascend out through the outlet conduit and not be trapped anywhere within the tank. Further, the removable lid 28 is capable of providing an air and fluid tight seal to the cell growth chamber.
FIG. 6 shows the reservoir tank 12 of the present invention. The reservoir tank comprises four side walls 32 defining a bottom 36 and an open top 70. The single piece reservoir tank 12 is also preferably injection molded, and therefore preferably has a slight outward draft to its side walls 32 to enable ejection from a mold cavity. The reservoir lid/growth tank (FIG. 4) fittingly engages the reservoir tank 12 thereby forming the top of the device 34, and is preferably permanently secured together with the reservoir tank 12 along the periphery of the top of the reservoir tank by any of a variety of fabrication methods including ultrasonic welding, press fitting, gluing, solvent welding, or even gaskets.
FIG. 7 shows a schematic diagram outlining the function of the cell culture device 11 of the present invention. The cell growth tank 14 is situated within the reservoir tank 12. The reservoir tank 12 holds media, the level of which is designated by a dotted line. Lines with arrows designate connections by hose, arrow directions depict the flow pattern of the media or air within these hoses. Media is drawn from the reservoir and through an air and liquid tight fitting 80 on the reservoir tank lid by a peristaltic pump 82, which serves as a circulation pump for the device. The media drawn from the reservoir is circulated through the pump, through a liquid and air tight fitting 84 on the lid of the reservoir tank. This fitting is attached to a tube 86 that projects into the reservoir tank. The bottom of this tube 86 is fitted with a connector hose that connects with the inlet conduit 64 on the bottom wall of the cell growth tank. Media is fed along this completely sealed route into the cell growth tank 14 and flows upwardly and evenly through the growth chambers created by the stacked plates. Upon reaching the top of the tank, the media is then fed out through an outlet conduit 30. A connecting hose links the outlet conduit on the cell growth tank lid to a specialized oxygenator nozzle 88 on the lid of the reservoir tank. The now deoxygenated media is next forced back into the reservoir and preferably sprayed against a side wall of the reservoir tank, which has been treated to be hydrophilic, and allowed to run down the side wall of the tank, into the media pool below as depicted by arrows leading from the oxygenator nozzle 88. As will be discussed later, the oxygenator nozzle 88 is specially designed to spray the wall of the reservoir tank in order to prevent foaming that would result if the media were allowed to fall directly into the pool.
To replenish oxygen, which is consumed by the cell growth process, air is introduced to the system, preferably by a common aquarium pump, through a tube connected to another fitting 90 on the top of the device. The pump provides sufficient air pressure for air to breathe out of the system through another fitting 92. Thereby, when media is running along the side wall, after passing through the oxygenator nozzle 88, it will pick up the fresh oxygen that is being pumped into the system. As an alternative to atmospheric air, pure oxygen may be pumped through fitting 90 or a combination of air and oxygen. Any air or oxygen entering the system is preferably first pumped through an air filter in order to preserve the sterility of the system. It should be noted that a pump is not the only means available to introduce gases to the system. The gas may be introduced by tanks or other means. The hose running from gas outlet fitting 92 is preferably fitted with a T-fitting connected to a pressure relief valve 91 to help compensate for any pressure build-up within the system.
From an external reservoir of fresh media, a tube runs through another peristaltic pump to fitting 94. This perfusion pump very slowly introduces fresh media into the system. Spent media is drawn from the system through a sipper tube 96 and out through fitting 98 via a third pump leading to a waste container. This third pump will preferably move more media in a given time than the perfusion pump. This insures that the level of the media in the reservoir tank always remains at the level of the bottom of the sipper tube 96.
The "circulation loop", which runs from the reservoir, through fitting 80, through the circulation pump 82, through fitting 84, tube 86, and into the cell growth tank through inlet conduit 64, is preferably fitted with a series of T-fittings 83. One set of T-fittings connects the loop on either side of the pump to a pressure relief valve 89 which helps ensure that excess pressure does not build in the system. The other two fittings in the circulation loop are used to add seed media to the cell growth chamber, as will be discussed below, or can be used to extend the loop for, through a detector or analyzer, for example.
FIG. 8 shows a preferred embodiment for a nozzle design that aids in oxygenation. As described earlier, it is important that deoxygenated media be exposed to oxygen. This is accomplished by allowing media to descend from the top the reservoir tank to the pool at the bottom. The cylindrically shaped oxygenator nozzle 88 has a solid bottom surface 100, thereby restricting downward flow. The radial side wall 102 of the nozzle has at least one aperture 104 through which a stream of media may be projected in a substantially horizontal direction. Once contacted with the side wall 32 of the reservoir tank, the liquid is held against the side wall by surface tension as it falls into the pool of media below. The side walls of the reservoir tank are preferably treated to be hydrophilic so that the media will spread out across the surface and adhere to the entire surface. An alternative to spraying the media against a side wall is to hang a screen or curtain of synthetic fabric or non-woven polyester material from the top of the reservoir tank, into the pool below, running substantially the length of the reservoir tank. The screen is also preferably hydrophilic. The media is sprayed against the screen, or alternatively allowed to fill a trough and spill over onto the screen, and held by surface tension, falls to the pool below. Foaming of the media is prevented in either case.
FIG. 9 is a cross section of the cell culture device of the present invention taken along the section line A--A in FIG. 2. Like features from previous figures are denoted by the same numerals.
FIG. 10 is a cross section of the cell culture device of the present invention taken along the section line B--B in FIG. 2. Like features from previous figures are denoted by the same numerals.
The bottom wall 60 of the cell growth tank 14 is designed to facilitate even flow into the individual chambers of the cell growth tank. The inlet conduit 64 directs flow into a trough 62 that runs perpendicular to the conduit along the bottom width of the tank. The trough 62 is defined by the radial groove formed at the bottom most part of the cell growth tank and the axial alignment of double triangular points 46 of each stacked plate. Media enters through the inlet conduit 64 and spreads laterally along the trough 62. It then is forced upwardly through the passages 45 and chambers formed by the separated and stacked plates 26. The general wedge shape of the cell growth tank 14 and plates facilitates uniform flow upwardly through the tank. The preferred intersecting or double triangular element 46 attributed to the plates helps centralize any air bubbles that enter the tank 14 and promote their exit through the restriction port, and eventually through the centralized outlet conduit nozzle 30 on the lid 28.
It is important to note that the cell growth system of the present invention is entirely self contained and can grow cells independently and without human intervention. Further, due to the closed loop nature of the system, the only sterile connections required in the system are the "media feed" fitting 94, through which fresh media enters the reservoir tank, and the two T-fittings used to seed the cell growth tank (FIG. 7). The sterility of the internal system environment cannot be threatened from outside contaminants through any other access fitting. The only other fitting that leads into the system from an outside source carries air or pure oxygen that has been forced through an air filter.
The walls of the reservoir tank, walls of the cell growth tank, and plates are preferably optically clear. The transparency provides several advantages. First, proper use and function of the device can be monitored by sight. Excess foaming can be detected, color of the media can be monitored, all internal plumbing fixtures can be observed, and cell growth can be analyzed. Second, since one side wall of the cell growth tank is preferably flush against or close to a side wall of the reservoir tank, a microscope may be used to analyze cells through the two overlapping transparent walls when the unit is laid on its side.
The position of the cell growth tank should be within the focal length of the microscope. Several steps are required for operation of the device. The first step is to attach seed cells to both sides of the 15 plates, plus the interior of the side walls 50 of the cell growth tank 14. This is accomplished by first clamping the hose that leads from the fitting 80, prior to the T-fittings, then introducing media containing the seed cells through T-fittings 83 in the circulation loop. Once the cell growth tank is filled with seed media, the hose leading from the outlet conduit 30 to the oxygenator nozzle 88 is clamped, thus isolating the growth tank from the reservoir tank 12. The device is then tipped over so that it lies with the plates in the growth tank lying in a horizontal plane. The seeds are heavier than the media and will therefore settle on one surface of the plates, and because the plates are specially treated, the cells will eventually attach to the plates such that they will stay in place when the device is returned to the vertical position.
The media is next drained from the growth tank, through a T-fitting, and the tank is flushed with fresh media. It is possible to have additional nozzles or fittings in the lid of the growth tank to help facilitate drainage of the seed media. Fresh seeded media is next introduced to the growth tank. The device is then tipped such that the plates are again horizontal, but so that opposite side of the plate now faces upward. In this manner, both sides of each plate can be seeded.
After a proper time has been allowed for the attachment of the cells, the device is turned to its upright position as shown in FIG. 2, and the seed media is again flushed out. Fresh media is introduced into the reservoir preferably through the neck on the reservoir tank lid, or alternatively through the T-fittings located within the tubing connecting the circulation pump. With attachment of the proper hoses and initiating the operation of the pumps, the device will operate indefinitely.
For the cells to grow and multiply, it is necessary for a continuous moving film of fresh media to move over them for nourishment. The media follows the circulation path as described earlier. The circulation path repeats continuously for the duration of the cell growth period, which may be as long as several weeks.
At the completion of the growth cycle, it is sometimes desirable to examine the individual plates, for example to study growth patterns using a microscope. It is also sometimes desirable to harvest the cells from the plates using a scraping tool. For these reasons, the lid to the growth tank is preferably removable.
Although the invention has been described in detail for the purpose of illustration, it is understood that such detail is solely for that purpose and variations can be made therein by those skilled in the art without departing from the spirit and scope of the invention which is defined by the following claims.
|Cited Patent
|Filing date
|Publication date
|Feb 8, 1972
|Dec 10, 1974
|Nat Res Dev
|Cell culture systems
|Jun 9, 1978
|Sep 30, 1980
|Battelle-Institut E.V.
|Process for the in-vitro biosynthesis of hormones, especially insulin
|Jul 13, 1978
|Oct 14, 1980
|Toray Industries, Inc.
|Cell culture propagation apparatus
|Feb 6, 1985
|May 26, 1987
|Vxr, Inc.
|Sparger and apparatus for and method of growing cells
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|Jul 28, 1987
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|International Classification
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|European Classification
|Sep 4, 1998
Owner name: CORNING INCORPORATED, NEW YORK
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:COUGHLIN, PAUL T.;LYMANN, GEORGE F.;MATHUS, GREGORY;REEL/FRAME:009434/0001;SIGNING DATES FROM 19980708 TO 19980713
|Jan 29, 2004
|Fee payment
Year of fee payment: 4
|Feb 22, 2008
|Fee payment
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|Mar 3, 2008
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text:
The PokéCommunity Forums
Off Topic Discussions
> Has your birthday gone by?
View Full Version :
Has your birthday gone by?
August 16th, 2009, 9:42 PM
Has your birthday ever gone by without the PC members even recognizing it. Happened to me to days ago. Im so hurt.
August 16th, 2009, 11:27 PM
Actually, my last birthday wasn't like that. In fact, I wasn't expecting to be recognized on my birthday. But the when logged on in the afternoon I got like 8 VMs wishing me a happy birthday o.o. Talk about an element of surprise. Jorah and many others that I knew and kinda knew.
It's because of my real life. I never expect to be recognized of anything because of the morons at my school.
August 16th, 2009, 11:29 PM
I haven't had a birthday while on PC yet. I joined a few weeks after my birthday, actually. XDD;
August 16th, 2009, 11:43 PM
eeh.. for my past birthday's on here I maybe got the occasional PM, that's about it though. Haven't had any birthdays pass since the VMs were released though, so I can't say much about that.
August 17th, 2009, 2:34 AM
My birthday was back in July, and I actually got several VMs from people I've spoken to over the past whatever, and a few random ones from people I didn't know. XD It made me feel special. x3
August 17th, 2009, 2:46 AM
Yes, but I have my actual birthdate hidden. Of course, it's easy to tell when someone's birthday is by looking at their age. Mine changed two days ago and no one even noticed. I feel so forgotten. TT~TT
Ayano Katagiri
August 17th, 2009, 2:55 AM
I've had three birthdays while I've been at PC I think and I got a few VM/PMs last time. I think I've had threads before, but they got chewed up by the DBE.
August 17th, 2009, 2:55 AM
I wouldn't know yet 0_0 My birthday isn't till September.. maybe some people will say Happy Birthday to me? xD
Jyn Erso
August 17th, 2009, 2:58 AM
3 Birthdays, no recognition.
Chances are I went on one of my 'vacations from PC' for them though so I dont mind.
August 17th, 2009, 3:00 AM
my birthday was last thursday the 13th of August and i didn't get any birthday wishes
didn't expect any though
August 17th, 2009, 3:15 AM
My first birthday here obviously went by without any recognition since I was very new. I had a thread and some VMs for my second one, which was very nice =P
.little monster
August 17th, 2009, 3:32 AM
No, I haven't been here long enough. However, in real life for my 13th birthday party my mom got this whole great party lined up, cost her a thousand dollars. I invited 30 people. Half of them claimed they forgot. ;o; I was grounded because of that too. That's great logic right there, ground your kid for not being liked/not controlling other peoples' memory.
August 17th, 2009, 3:33 AM
My birthday was around January 3rd, so it's passed...or approaching, could very well go both ways. No, I wasn't forgotten. But I do feel for those who have been forgotten when their special day arrived. :/
August 17th, 2009, 4:46 AM
I actually remove my birthday from PC a few days in advance so that someone I know can make my thread as opposed to the first Australian noob that sees my name at the bottom of the page. v_v;; I'll make it public again when I have a thread. I usually mention birthday plans and the like to friends before the day so usually someone remembers. (And if not, I'm sure LJ sends them annoying emails about it.)
Yes, but I have my actual birthdate hidden. Of course, it's easy to tell when someone's birthday is by looking at their age. Mine changed two days ago and no one even noticed. I feel so forgotten. TT~TT
...happy belated birthday! :( I never notice ages so I wouldn't notice something subtle like that, ahaha.
Ben Kenobi
August 17th, 2009, 5:45 AM
All 3 of my birthdays since I've joined here have gone unnoticed.
August 17th, 2009, 6:06 AM
Two members wished me happy birthday a few days ago. I'm so happy! :D
August 17th, 2009, 8:50 AM
Yes I was forgotten the 1st time due to not knowing anyone. I'll probably get a birthday thread this year! (Hopefully)
August 17th, 2009, 8:56 AM
Nope not yet, in a while it will be my bday :D
August 17th, 2009, 9:18 AM
Yeah, it went by. No one VM'd me about it, but I think someone said something in a thread? I don't remember. My memory fails me half the time. XD;;
August 17th, 2009, 10:01 AM
I've never had birthday greetings even though two birthdays have gone by since I joined PC (to be fair, I wasn't active when I joined, the month before the first birthday).
It doesn't really bother me. =V
August 17th, 2009, 2:17 PM
No my birthday hasn't come up yet.
Although its only 22 days away. :)
August 17th, 2009, 2:22 PM
I joined after my birthday
so i haven't had a b-day at PC yet :B
I better get happy birthday wishes though or I'm gonna have to shank some people in their sleep D:< /slapped /terrible person xD;; LMFAO
August 17th, 2009, 3:06 PM
I joined a few weeks after my birthday, and I haven't even passed a year yet, so there's no way that's happened yet.
Even if I did join before my birthday I'll probably get nothing due to being new.
August 17th, 2009, 5:46 PM
My birthday has gone by twice since I've joined PC. Each time, I've fortunately gotten vms and a thread. It's made me really appreciate how great a lot of people truly are. :]
August 17th, 2009, 5:53 PM
MINE'S IN TWO DAYS! MINE'S IN TWO DAYS!!! Everyone has to get me sour punch straws!! \o/
edit; last year there was maintenance going on and I couldn't get online on my birthday. =[
August 17th, 2009, 10:16 PM
I've only had one birthday here at PC, and people recognised it. Even some random people just posted on my profile wishing me a happy birthday. I hope I get a thread next year. *wink wink*
August 17th, 2009, 11:39 PM
I've had two birthdays at PC, wasn't noticed on either of em. I don't understand why, I post a lot and I've made lots of friends here... :P
I've never cared for birthdays anyway, so s'all good.
August 17th, 2009, 11:46 PM
I've had my 17th and 18th birthday on PC, both got noticeable threads. Not trying to boast or anything, it's just that my friends cared to enough to spam. XD; I really appreciate those who noticed though, even if it's not that big of a deal.
August 18th, 2009, 12:24 AM
I was never forgotten. I got this neat little message that said PC congratulated me!
but yeah, other than the whole forum congratulating me, I only got one message telling me happy birthday.
I guess the rom hacking section wasn't exactly a huge, mega popular place >_>
August 18th, 2009, 12:32 AM
Well today is my birthday :classic:
and i got a happy birthday form Luck who i don't even know:) but thats it
I'm so happy:)
Edit: 3 people so far:)
August 18th, 2009, 1:00 AM
Yeah, My birthday passed. Not that long ago,about a month. I didn't get any "Happy Birthdays" from any PC members,only the e-mail. I don't mind though,I was new.
August 18th, 2009, 1:02 AM
I actually received a nice-sized thread and good number of VMs for my birthday last year. Which surprised me, to be frank. I wasn't even remotely aware that so many people actually cared enough about me to spam up a thread. XD;
I don't think it's going to be quite so extravagant this year... I haven't been around as much. :x
~*!*~Tatsujin Gosuto~*!*~
August 18th, 2009, 7:16 AM
happy Birthday Bzuma
ummm, My birthday is on January 6th, and only 6 people recognized it but then again I wasn't active at the time of my B-day because of studying for Finals at the time
August 18th, 2009, 12:40 PM
Um... first birthday here a thread was made, but only like three people replied XD Second year no thread was made, but I got at least 20 PMs wishing me happy birthday. Third year a thread was made, and it got a fair number of replies :O As for this year, my birthday isn't for another month or so XD
Both times somebody special made my thread, and I would be kind of miffed if some random person ever snagged it instead of a friend XD;;
August 18th, 2009, 12:51 PM
One birthday so far, got three replies, but it's gooood. Next birthday is in Oct, so should get at least a couple more than last year.
August 18th, 2009, 1:15 PM
I've had one, and I got a few VMs, but that was early days at PC.
August 18th, 2009, 2:01 PM
My 17th birthday passed on the third of May, but I did have my first birthday thread made for me here by Katherine. It was pretty nice and I didn't get to feel lonely that day here.
Hint: I'm Aero. aka f l a s h, remember...
August 18th, 2009, 3:36 PM
Yes. It happens all the time but I don't really care. There is a lot of people on PC and I don't know the 'popular' people so those people don't remember so they don't make a thread for me. Though I got to the point where I don't care nor does it ruin the day.
Now excuse me as I go to the corner and cry to be reminded that nobody remembered my birthday this year.
August 18th, 2009, 3:47 PM
Yes it's happened my birthday was November 14th only one person replied to the thread I made. It doesn't matter though.


text:
/// Information Diet: Carole Radziwill
Specs Age 49 Accomplishments Stars on Bravo's The Real Housewives of New York City ; Glamour columnis t ; author of The Widow's Guide to Sex & Dating (spring 2013) and The New York Times best-seller What Remains: A Memoir of Fate, Friendship and Love ; former ABC News producer. Base New York What's the first information you consume in the morning? I open my eyes around 8:30 a.m., but I like to tiptoe into the day. So I lounge around in bed listening to NPR in the background. I like the sound of reporters' voices. They are soothing. If anything earth-shattering has happened overnight, I'll hear about it even in my semi-sleep. Then I check texts, email and Twitter, all from bed. What do you read or watch or listen to at the breakfast table? When I get up, I get coffee and skim the New York Post to see if anyone I know got themselves involved in a scandal. I don't eat breakfast or have a breakfast table, but if I did, I'd have a morning show on in the background and be reading a magazine. I don't like my news too instant; it's like someone yelling all the time. I lean toward weeklies like Newsweek or Time . The information is delayed a bit, nonessentials are weeded out, sources have been checked. What a novel idea. What occupies your mind in the car, on the subway, train or bus? Stories. I just finished my first novel and am starting another, so I'm living in imaginary places with imaginary people all the time.
Here is the original post:
Information Diet: Carole Radziwill
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text:
Tracy, who doesn't have a blog, lives in Germany so if you win her donation expect to wait a bit longer to receive it, makes reversible little girl bonnets.
When she showed me photos of them I squealed, fondly remembering my days when I was obsessed with Little House on the Prairie.
I think all little girls would love an old fashioned bonnet like this.
735 views
1 fave
0 comments
Taken on August 11, 2004


text:
President Barack Obama, in San Jose on Friday, defended U.S. surveillance of telecommunications records.
He arrived in Silicon Valley on Thursday to raise money and stayed Friday to tout California's participation in the Affordable Care Act.
Protesters confronted him Thursday about the Keystone XL fuel pipeline, and reporters questioned him Friday about government snooping.
Speaking at the Fairmont Hotel in San Jose, Obama praised California for how it's implementing the Affordable Care Act, also known as Obamacare.
He said the new law will expand coverage through Covered California, the state-run insurance exchange. And he urged people not to focus on the criticisms of the plan.
"So, the bottom line is you can listen to a bunch of political talk, negative ads," he said. "Or alternatively you can actually look at what's happening in states like California right now."
President Obama also said the new law will cover more than 10 million uninsured Latinos, many of whom live in California.
After his remarks, reporters focused on recent reports that U.S. security agencies have requested records of Americans' phone and Internet usage.
From The Associated Press:
SAN JOSE, Calif. (AP) — President Barack Obama is defending his government's secret surveillance, saying Congress has repeatedly authorized the collection of America's phone records and U.S. Internet use.
In his first comments since the programs were publicly revealed this week, Obama says safeguards are in place. He says nobody is listening to the content of phone calls. And he says the Internet targeting is aimed at foreign nationals, not American citizens.
Obama says he increased some of the "safeguards" on the programs after taking office. And he believes they help his administration stop terrorist attacks.
Tweets from reporters covering the speech:
Obama now refers to his War on Terror over speech 2 weeks ago. "I welcome this debate." Cue: politico.com/story/2013/06/…
— Michelle Malkin (@michellemalkin) June 7, 2013
POTUS in San Jose: "You can't have 100% security and then 100% privacy and 0% inconvenience." Laments tradeoffs.
— John Myers (@johnmyers) June 7, 2013
"When it comes to telephone calls, nobody is listening to your telephone calls," says Obama in San Jose on NSA surveillance controversy..
— John Myers (@johnmyers) June 7, 2013
Obama: CA officials work to implement #Obamacare "have shown results in the biggest insurance market in the country"
— John Myers (@johnmyers) June 7, 2013
From the San Francisco Chronicle:
President Obama swooped through Silicon Valley Thursday for two private fundraisers, dogged by protesters who urged him not to approve the controversial Keystone XL pipeline project that would transport tar sands fuel across the United States.
Holding signs that said "Stop the Keystone XL pipeline" and chanting "Hey Obama. We don't want no pipeline drama," more than 350 demonstrators from activist groups, including CREDO Mobile and the Sierra Club, gathered near the Palo Alto home of Flipboard founder and CEO Michael McCue.
Background from The Associated Press:
SAN JOSE, Calif. (AP) — President Barack Obama is making a personal plea to Californians, Latinos and young people to sign up for coverage under the new health care law.
Obama on Friday will use his visit to California to highlight how the state is implementing the Affordable Care Act. He also will discuss California's collaboration with non-government groups to promote, primarily to Hispanics, the health care exchanges that are being created to help millions of now-uninsured consumers afford coverage, White House officials said.
California has the country's biggest insurance market and, with 6 million uninsured residents, it is a crucial part of the effort to get consumers to sign up for coverage, officials said.
Thirteen insurance companies will be offering multiple health plans that vary in coverage and price through California's exchange, even in some of the most rural regions of the state.
Getting young people to enroll through the exchanges also is critical; they cost insurers less money because they tend to have the best health and don't require a lot of costly medical care.
The Obama administration is looking for about 7 million people to enroll through the exchanges, and 2.6 million of them need to be younger in order to keep costs down for the overall pool of enrollees, officials said. Nearly one-third of these young people live in three states: California, Texas and Florida.
Among the private entities working with the state of California to promote enrollment are the Spanish-language TV networks Telemundo and Univision. The White House says the law will give more than 10 million uninsured Latinos across the country the opportunity to afford health insurance coverage.
Not all states are pushing the law. In many states, Republican governments are opposing the law's implementation and are leaving oversight of the exchanges to the federal government.
Obama arrived in California on Thursday evening to attend fundraisers in Palo Alto and Portola Valley to help Senate Democratic candidates.
The fundraising was to continue Friday with Obama's attendance at two Democratic National Committee fundraisers at the Santa Monica home of Peter Chernin, a former News Corp. executive, officials said. The events include a lunch for about 100 people, followed by a smaller 35-person reception. Tickets for the events range from $10,000 to $32,400.


text:
Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
Looking to draft! If you are interested in an invite please like this shot by Saturday 1/27/13, 11:59pm MST. Just make sure there is a link to your portfolio in your Dribbble profile and I will review everyone's and make a selection the following day :)
Can't wait to see everyone's work!
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