text:
The Library
Browse by Warwick Author
|Up a level
Group by: Official Date | Item Type | Funder | No Grouping
Number of items: 44.
Hu, Jiamiao, Kyrou, Ioannis, Tan, Bee K., Dimitriadis, Georgios K., Ramanjaneya, Manjunath, Tripathi, Gyanendra, Patel, Vanlata H., James, Sean, Kawan, Mohamed, Chen, Jing and Randeva, Harpal S.. (2016) Short-chain fatty acid acetate stimulates adipogenesis and mitochondrial biogenesis via GPR43 in brown adipocytes. Endocrinology . en.2015-1944. ISSN 0013-7227 (In Press)
Davies, Julie, Chen, Jing, Pink, Ryan, Carter, David, Saunders, Nigel, Sotiriadis, Georgios, Bai, Bo, Pan, Yanyou, Howlett, David, Payne, Annette, Randeva, Harpal S. and Karteris, Emmanouil. (2015) Orexin receptors exert a neuroprotective effect in Alzheimer's disease (AD) via heterodimerization with GPR103. Scientific Reports, 5 . pp. 1-12. 12584. ISSN 2045-2322
Chen, Jing, Zhang, Rumin, Chen, Xiaoyu, Wang, Chunmei, Cai, Xin, Liu, Haiqing, Jiang, Yunlu, Liu, Chuanxin and Bai, Bo. (2015) Heterodimerization of human orexin receptor 1 and kappa opioid receptor promotes protein kinase A/cAMP-response element binding protein signaling via a Gαs-mediated mechanism. Cellular Signalling, 27 (7). pp. 1426-1438. ISSN 0898-6568
Zhou, Zhichao, Chen, Jing, Cao, Huiluo, Han, Ping and Gu, Ji-Dong. (2015) Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes. Frontiers in Microbiology, 5 . 789. ISSN 1664-302X
Chen, Jing, Zhou, Zhichao and Gu, Ji-Dong. (2015) Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes. Applied Microbiology and Biotechnology, 99 (3). pp. 1463-1473. ISSN 0175-7598
Chen, Jing, Jiang, Xi-Wen and Gu, Ji-Dong. (2015) Existence of novel Phylotypes of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in surface and subsurface sediments of the South China Sea. Geomicrobiology Journal , 32 (1). pp. 1-10. ISSN 0149-0451
Tan, Bee K., Chen, Jing, Hu, J., Amar, Omar, Mattu, Harman, Ramanjaneya, Manjunath, Patel, Vanlata H., Lehnert, Hendrik and Randeva, Harpal S.. (2014) Circulatory changes of the novel adipokine adipolin/CTRP12 in response to metformin treatment and an oral glucose challenge in humans. Clinical Endocrinology, 81 (6). pp. 841-846. ISSN 0300-0664
Bai, Bo, Jiang, Yunlu, Cai, Xin and Chen, Jing. (2014) Dynamics of apelin receptor/G protein coupling in living cells. Experimental Cell Research, Volume 328 (Number 2). pp. 401-409. ISSN 0014-4827
Ban, Bo, Bai, Bo, Zhang, Manman, Hu, Jiamiao, Ramanjaneya, Manjunath, Tan, Bee K. and Chen, Jing. (2014) Low serum cartonectin/CTRP3 concentrations in newly diagnosed type 2 diabetes mellitus : in vivo regulation of cartonectin by glucose. PLoS One, 9 (11). pp. 1-5. e112931. ISSN 1932-6203
Bai, Bo, Cai, Xin, Jiang, Yunlu, Karteris, Emmanouil and Chen, Jing. (2014) Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2and cell proliferationviaGαq-mediated mechanism. Journal of Cellular and Molecular Medicine, Volume 18 (Number 10). pp. 2071-2081. ISSN 1582-1838
Chen, X., Bai, B., Tian, Y., Du, H. and Chen, Jing. (2014) Identification of serine 348 on the apelin receptor as a novel regulatory phosphorylation site in apelin-13-induced G protein-independent biased signaling. Journal of Biological Chemistry . ISSN 0021-9258
Bai, Bo, Liu, Lulu, Zhang, Ning, Wang, Chunmei, Jiang, Yunlu and Chen, Jing. (2014) Heterodimerization of human apelin and bradykinin 1 receptors : novel signal transduction characteristics. Cellular Signalling, Volume 26 (Number 7). pp. 1549-1559. ISSN 0898-6568
Chen, Jing, Zhou, Zhi-Chao and Gu, Ji-Dong. (2014) Occurrence and diversity of nitrite-dependent anaerobic methane oxidation bacteria in the sediments of the South China Sea revealed by amplification of both 16S rRNA and pmoA genes. Applied Microbiology and Biotechnology, 98 (12). pp. 5685-5696. ISSN 0175-7598
Patel, Vanlata H., Adya, Raghu, Chen, Jing, Ramanjaneya, Manjunath, Bari, Muhammad F., Bhudia, Sunil K., Hillhouse, Edward W., Tan, Bee K. and Randeva, Harpal S.. (2014) Novel insights into the cardio-protective effects of FGF21 in lean and obese rat hearts. PLoS One, Volume 9 (Number 2). Article number e87102. ISSN 1932-6203
Wang, Chunmei, Pan, Yanyou, Zhang, Rumin, Bai, Bo, Chen, Jing and Randeva, Harpal S.. (2014) Heterodimerization of mouse orexin type 2 receptor variants and the effects on signal transduction. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843 (Number 3). pp. 652-663. ISSN 0167-4889
Tan, Bee K., Chen, Jing, Hu, Jiamiao, Amar, Omar, Mattu, Harman, Adya, Raghu, Patel, Vanlata H., Ramanjaneya, Manjunath, Lehnert, Hendrik and Randeva, Harpal S.. (2013) Metformin increases the novel adipokine cartonectin/CTRP3 in women with polycystic ovary syndrome. The Journal of Clinical Endocrinology & Metabolism, 98 (12). E1891-E1900. ISSN 0021-972X
Ramanjaneya, Manjunath, Karteris, Emmanouil, Chen, Jing, Rucinski, R., Ziolkowska, A., Ahmed, Naila, Kagerer, S., Johren, O., Lehnert, Hendrik, Malendowicz, L. K. and Randeva, Harpal S.. (2013) QRFP induces aldosterone production via PKC and T-type calcium channel-mediated pathways in human adrenocortical cells : evidence for a novel role of GPR 103. American Journal of Physiology: Endocrinology and Metabolism, 305 (9). E1049-E1058. ISSN 0193-1849
Tan, Bee K., Chen, Jing, Adya, Raghu, Ramanjaneya, Manjunath, Patel, Vanlata H. and Randeva, Harpal S.. (2013) Metformin increases the novel adipokine adipolin/CTRP12 : role of the AMPK pathway. Journal of Endocrinology, Volume 219 (Number 2). pp. 101-108. ISSN 0022-0795
Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2012) Protective actions of globular and full-length adiponectin on human endothelial cells : novel insights into adiponectin-induced angiogenesis. Journal of Vascular Research, 49 (6). pp. 534-543. 338279. ISSN 1018-1172
Punn, Anu, Chen, Jing, Delidaki, Maria, Tang, Jiyou, Liapakis, George, Lehnert, Hendrik, Levine, Michael A. and Grammatopoulos, Dimitris K.. (2012) Mapping structural determinants within third intracellular loop that direct signaling specificity of type 1 corticotropin-releasing hormone receptor. Journal of Biological Chemistry, Volume 287 (Number 12). pp. 8974-8985. ISSN 0021-9258
Huang, Taipeng, Chen, Jing, Yang, Qiuyan, Zhang, Yongkui and Ding, Ranfeng. (2012) Biodegradation of heavy oil with high asphaltene content by Kocuria sp. Asian Journal of Chemistry, 24 (6). pp. 2717-2720. ISSN 0970-7077
Chen, Jing, Huang, P. T., Zhang, K. Y. and Ding, F. R.. (2012) Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil. Journal of Applied Microbiology, 112 (4). pp. 660-671. ISSN 1364-5072
Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2012) Protective actions of globular and full-length adiponectin on human endothelial cells : novel insights into adiponectin-induced angiogenesis. Journal of Vascular Research, Vol.49 (No.6). pp. 534-543. ISSN 1018-1172
Chen, Jing, Yang, Qiuyan, Huang, Taipeng, Zhang, Yongkui and Ding, Ranfeng. (2011) Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation. World Journal of Microbiology and Biotechnology, 27 (6). pp. 1381-1389. ISSN 0959-3993
Tan, Bee K., Adya, Raghu, Chen, Jing, Lehnert, Hendrik, Cassia, Louis J. Sant and Randeva, Harpal S.. (2011) Metformin treatment exerts antiinvasive and antimetastatic effects in human endometrial carcinoma cells. Journal of Clinical Endocrinology & Metabolism , Vol.96 (No.3). pp. 808-816. ISSN 0021-972X
Tan, B. K., Chen, J., Adya, Raghu and Randeva, Harpal S.. (2011) Phosphoprotein enriched in diabetes gene product (Ped/pea-15) is increased in omental adipose tissue of women with the polycystic ovary syndrome : ex vivo regulation of ped/pea-15 by glucose, insulin and metformin. Diabetes, Obesity and Metabolism, Vol.13 (No.2). pp. 181-184. ISSN 1462-8902
Ramanjaneya, Manjunath, Conner, Alex C., Brown, James E. P., Chen, Jing, Digby, Janet E., Barber, T. (Thomas), Lehnert, Hendrik and Randeva, Harpal S.. (2011) Adiponectin (15–36) stimulates steroidogenic acute regulatory (StAR) protein expression and cortisol production in human adrenocortical cells : role of AMPK and MAPK kinase pathways. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1813 (Number 5). pp. 802-809. ISSN 0167-4889
Karteris, Emmanouil, Markovic, Danijela, Chen, Jing, Hillhouse, Edward W. and Grammatopoulos, Dimitris K.. (2010) Identification of a novel corticotropin-releasing hormone type 1 beta-Like receptor variant lacking exon 13 in human pregnant myometrium regulated by estradiol-17 beta and progesterone. Endocrinology, Vol.151 (No.10). pp. 4959-4968. ISSN 0013-7227
Patel, Vanlata H., Chen, Jing, Ramanjaneya, Manjunath, Karteris, Emmanouil, Zachariades, Elena, Thomas, Peter, Been, Martin and Randeva, Harpal S.. (2010) G-protein coupled estrogen receptor 1 expression in rat and human heart : protective role during ischaemic stress. International Journal of Molecular Medicine, Vol.26 (No.2). pp. 193-199. ISSN 1107-3756
Ramanjaneya, Manjunath, Chen, Jing, Brown, James E. P., Tripathi, G. N., Hallschmid, Manfred, Patel, Suketu, Kern, Werner, Hillhouse, Edward W., Lehnert, Hendrik, Tan, Bee K. and Randeva, Harpal S.. (2010) Identification of nesfatin-1 in human and murine adipose tissue: a novel depot-specific adipokine with increased levels in obesity. Endocrinology, Vol.151 (No.7). pp. 3169-3180. ISSN 0013-7227
Chen, Jing and Randeva, Harpal S.. (2010) Genomic organization and regulation of the human orexin (hypocretin) receptor 2 gene : identification of alternative promoters. Biochemical Journal, Vol.427 (No.Part 3). pp. 377-390. ISSN 0264-6021
Kaur, Jaspreet, Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2010) Identification of chemerin receptor (ChemR23) in human endothelial cells: chemerin-induced endothelial angiogenesis. Biochemical and Biophysical Research Communications, Vol.391 (No.4). pp. 1762-1768. ISSN 0006-291X
Tan, Bee K., Chen, Jing, Farhatullah, S., Adya, Raghu, Kaur, Jaspreet, Heutling, Dennis, Lewandowski, Krzysztof C., O'Hare, J. Paul, Lehnert, Hendrik and Randeva, Harpal S.. (2009) Insulin and metformin regulate circulating and adipose tissue chemerin. Diabetes, Vol.58 (No.9). pp. 1971-1977. ISSN 0012-1797
Tan, Bee K., Adya, Raghu, Chen, Jing, Farhatullah, Syed, Heutling, Dennis, Mitchell, Daniel Anthony, Lehnert, Hendrik and Randeva, Harpal S.. (2009) Metformin decreases angiogenesis via NF-kappa B and Erk1/2/Erk5 pathways by increasing the antiangiogenic thrombospondin-1. Cardiovascular Research, Vol.83 (No.3). pp. 566-574. ISSN 0008-6363
Tan, Bee K., Chen, Jing, Brown, James, Adya, Raghu, Ramanjaneya, Manjunath, Menon, Vinod, Bailey, C. J. (Clifford J.), Lehnert, Hendrik and Randeva, Harpal S.. (2009) In vivo and ex vivo regulation of visfatin production by leptin in human and murine adipose tissue : role of mitogen-activated protein kinase and phosphatidylinositol 3-kinase signaling pathways. Endocrinology, Vol.15 (No.8). pp. 3530-3539. ISSN 0013-7227
Ramanjaneya, Manjunath, Conner, Alex C., Chen, Jing, Kumar, Prashanth, Brown, James E. P., Joehren, Olaf, Lehnert, Hendrik, Stanfield, Peter R. and Randeva, Harpal S.. (2009) Orexin-stimulated MAP kinase cascades are activated through multiple G-protein signalling pathways in human H295R adrenocortical cells : diverse roles for orexins A and B. Journal of Endocrinology, Vol.202 (No.2). pp. 249-261. ISSN 0022-0795
Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2009) Pre-B cell colony enhancing factor (PBEF)/visfatin induces secretion of MCP-1 in human endothelial cells : role in visfatin-induced angiogenesis. Atherosclerosis, Vol.205 (No.1). pp. 113-119. ISSN 0021-9150
Tang, Jiyou, Chen, J. (Jing), Ramanjaneya, Manjunath, Punn, Anu, Conner, Alex C. and Randeva, Harpal S.. (2008) The signalling profile of recombinant human orexin-2 receptor. Cellular Signalling, Vol.20 (No.9). pp. 1651-1661. ISSN 0898-6568
Ramanjaneya, Manjunath, Conner, Alex C., Chen, J. (Jing), Stanfield, Peter R. and Randeva, Harpal S.. (2008) Orexins stimulate steroidogenic acute regulatory protein expression through multiple signaling pathways in human adrenal H295R cells. Endocrinology, Volume 149 (Number 8). pp. 4106-4115. ISSN 0013-7227
Tan, Bee K., Heutling, Dennis, Chen, Jing, Farhatullah, S., Adya, Raghu, Keay, Stephen D., Kennedy, C. Richard, Lehnert, Hendrik and Randeva, Harpal S.. (2008) Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance. Diabetes, Vol.57 (No.6). pp. 1501-1507. ISSN 0012-1797
Adya, Raghu, Tan, Bee K., Punn, Anu, Chen, Jing and Randeva, Harpal S.. (2008) Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways : novel insights into visfatin-induced angiogenesis. Cardiovascular Research, Vol.78 (No.2). pp. 356-365. ISSN 0008-6363
Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2008) Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation. Diabetes Care, Vol.31 (No.4). pp. 758-760. ISSN 0149-5992
Tan, Bee K., Chen, Jing, Lehnert, Hendrik, Kennedy, Richard and Randeva, Harpal S.. (2007) Raised serum, adipocyte, and adipose tissue retinol-binding protein 4 in overweight women with polycystic ovary syndrome: effects of gonadal and adrenal steroids. Journal of Clinical Endocrinology & Metabolism , Vol.92 (No.7). pp. 2764-2772. ISSN 0021-972x
Mistry, T., Digby, J. E., Chen, Jing, Desai, K. M. and Randeva, Harpal S.. (2006) The regulation of adiponectin receptors in human prostate cancer cell lines. Biochemical and Biophysical Research Communications, Vol.348 (No.3). pp. 832-838. ISSN 0006-291X
This list was generated on Mon Feb 27 13:48:30 2017 GMT.


text:
ADB is helping India extend the first metro train line in the heritage city of Jaipur and draw up plans to build a second line for the north-south corridor. The project will add a 2.3-kilometer underground section of the metro from Chandpole to Badi Chopar, along with two stations, which will provide access to the central business district.
Latest Project Documents
Consulting Notices See also: CMS
No notices are currently available for this project.
Procurement Notices See also: Operational Procurement
No notices are currently available for this project.
Procurement Documents
|Jaipur Metro Rail Line 1-Phase B Project: Procurement Plan
|Jul 2016
Project Data Sheets (PDS) contain summary information on the project or program. Because the PDS is a work in progress, some information may not be included in its initial version but will be added as it becomes available. Information about proposed projects is tentative and indicative.
The Public Communications Policy (PCP) recognizes that transparency and accountability are essential to development effectiveness. It establishes the disclosure requirements for documents and information ADB produces or requires to be produced.
The Accountability Mechanism provides a forum where people adversely affected by ADB-assisted projects can voice and seek solutions to their problems and report alleged noncompliance of ADB's operational policies and procedures.
In preparing any country program or strategy, financing any project, or by making any designation of, or reference to, a particular territory or geographic area in this document, the Asian Development Bank does not intend to make any judgments as to the legal or other status of any territory or area.

subset: commoncrawl


text:
Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
Hi fellows! Check out the new icon. Continue to experiment with baby theme :) Made it just for fun and practice with simple shapes and masked photo textures (using iphone camera and the real pram as a source).
Check out @2X and the process for more details.
keyboard shortcuts:
L or F like
Z toggle zoom
post comment
Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2017 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


text:
More Videos...
NMCA Gateway International
Gateway International Speedway - 1965 Ford Fairlane Ricart Racing 2010
Grant Klohn - 67 Fairlane 4 spd.
Sunday May long weekend at Mission Raceway Park
Dodge Dart vs Ford Fairlane at National Trails
This is one of the drag racing match ups at the Goodguys Friday Night Drags at National Trail Raceway in Hebron, Ohio that took place in 2012. Wrench Moran Facebook Hub- http://www.facebook.com/pages/Wrench-Moran/580062675345380 Tractor Pulling Facebook Hub- http://www.facebook.com/pages/Tractor-Pulls/243781515784304 Rough Truck Facebook Hub- http://www.facebook.com/RoughTrucks
Doorslammer Street Car 67' Fairlane - 2013 Thunder In The Valley, Port Alberni, BC.
Licensed street driven 67' Fairlane qualifies as a doorslammer, SWEET!!!


text:
Thousands upon Thousands Americans on the Medicare program are discovering that with the correct Medigap Coverage they will be able to:
Get a Medigap Policy today with the protection you want at a price that fits your budget.
Protect your physical health and secure your fiscal health with a Medicare Supplemental Insurance Plan
Like the many of people that our company have assisted since we first began years ago, you assuredly seen that attempting to make the decision on the ideal Medigap Policy is aggravating. Plus, finding a Medigap Broker to coordinate with in studying costs can be nerve-racking.
Happily, it doesn't have to be. Locating a Medicare Supplemental Company that that is trustworthy is no bother after you find out that:
Get a Medicare Supplemental Policy through our company and obtain peace of mind about both your affordable costs and the excellent protection.
Moreover that's not all our company is willing to do for you. Did you ever tried before to register for an insurance plan like a Medicare Supplement? Hundreds of thousands of people 65 years of age and older are sadly declined coverage or gotten limited coverage on their policy that they caught unawares once they had taken care of the initial premium. There can be stressful hassles involved in collecting a refund or finding other suitable Medicare Supplemental Insurance Coverage or other plans.
Things like this don't happen under our watch. The Medigap Agents we've hired are also highly trained "field underwriters.†So we'll not only aid you to locate the best for Medicare Supplement Costs, but we'll make certain you will meet the provider's requirements for the that particular plan first, then we'll get to the paperwork.. With a couple of quick quick questions about your health and medical history, you could instantly approved for Medigap Insurance Coverage that will accept you immediately , without hassles or anxieties of canceled policies.
It our policy, we do not counsel a specific policy for you unless we are certain you qualify for approval by the Medicare Supplemental Insurance company Furthermore, our Lowest Rate Guarantee means that you never ever pay anything for our services and are always making payments the affordable available rate on the suggested policy you qualify for.
What's more, for your convenience, we have made arrangements with all of our Medicare Supplemental Insurance Providers to make it easy for you apply for their plans right over the phone in just minutes. You receive the least expensive Medicare Supplement Insurance Prices available in your area, protection for life and, if you pick a Medicare Supplement Insurance Plan F, you will never ever pay a penny going forward for Medicare-covered medical necessities like x-rays, therapy, exams, prescription medication, etc..
Medicare Supplemental Insurance is also known as a "Medigap Planâ€
Medigap is another name given to health insurance plans called Medicare Supplements. Medigap Insurance Plans supplement the gaps in Medicare health protection left by Medicare Part A and Medicare Part B.
A Medigap Insurance Plan is not an stand-in for traditional Medicare like a Medicare Advantage Policy, instead it works side-by-side with traditional Medicare. And, Medigap Plans are different from Medicare Advantage Plans because they have no deductibles, no co-pays and no issues with being "in nework" that could impede you healing when you can't see the right doctor.
When you opt for the perfect Medicare Supplemental Medicare Supplemental Policy like Medicare Supplement Plan F you will never expect to fork over a single dime when you get medicare care in a physician's office, hospital or specialty treatment center that accepts Medicare.
This means you sign up for a Medicare Supplement Plan F, whether your health care expenses are massively huge, you will never ever have to pay any additional bills for health care expenses when Original medicare green-lights a penny of the charges.
Medicare Insurance Policies: How they give you freedom
When you require healthcare assistance with a specialty treatment center, doctor or hospital, simply show them your Medicare Supplemental card to the doctor, specialty treatment center or hospital. Then everything will, almost magically, be taken care of for you.
The fact is that, when your claim is submitted with Medicare for Medicare Part B charges, Medicare will in most cases reroute your claim direct to your Medigap Insurance Company without you needing to do anything. After that, your Medicare Supplement Carrier will take care of the claim, ordinarily without it ever touching human hands
With a easy comparison of Medigap Quotes, you will learn about the one that's right for health, finances and other life situations and then you won't have to worry about extra medical expenses ever again.. Again, if you select a Medigap Insurance Plan F from our company, you will never, ever get remaining healthcare expenses to deal with.
Our company stroungly believes that physicians out to be permitted prescribe what the absolute best medical attention is for you while your Medigap Plans guarantees you aren't weighed down with health care expenses. Also, when you enroll a Medicare Supplement Insurance Policies like a Medicare Supplemental Plan F it's YOURS FOREVER as long as you keep paying the premiums. Unlike Medicare Advantage Plans, provided that you keep on paying the monthly premiums, the Medicare Supplement Company can never, ever expropriate that policy away from you… EVER!
Just like all the thousands and thousands of people that we have assisted over the years, you have discovered that looking reach a decision on the right Medicare Supplement Insurance Plans is usually stressful right?. And, attempting to obtain a Medicare Supplemental Provider who will help out when assessing Medicare Insurance Rates is often difficult.
Fortunately, not every brokerage is like this. Working with a a Medicare Supplement Insurance Plan Broker whom is worthy of your trust is no problem because:
Plus there's no need to pay for our services.
Medicare Insurance Plans: Why purchase through our company?
If you are on Medicare, you're possibly encountering crucial final decisions about choosing the ideal Medicare Supplement Insurance Policy and you're possibly being taken for a ride by Medicare Advantage salespeople.
Medicare Supplemental Policy Companies are all mandated extend the same coverage options. Therefore it's so very, very important to compare and contrast Medicare Supplement Insurance Plan Rates from the leading providers with a brokerage you can trust.
We're different, because we work for you, not an individual Medicare Insurance Policy Carrier. licensensed agents scrutinize every of the Medigap Policy Providers that sell Medicare Insurance Quotes and only work with the good ones that have a good reputation and also they will be able to honor their commitments forever. It's pretty simple,, licensensed agents rejects the insignificant Medicare Supplement Insurance Policy Carriers and the unprofessional.
Once we've found the right Medigap Providers that match our rigid terms and stipulations, we employ our state of the art and our proprietary software to make Medicare Supplemental Policy Comparisons. Thanks to our exhaustive research, our licensed agents find the best deals for your particular situation based on your specific needs, not what the Medicare Supplement Policy Companies want you will buy.
Furthermore that is not all that our agents want to do. Ever tried to take out a policy like a Medicare Supplemental Insurance Policy? Many who do, are denied coverage or gotten restrictions on their policy that they didn't see coming only after they'd paid the first bill. Unfortunately there's often great hassles in getting the money you put down for the first premium or signing up for different Medicare Supplement Insurance Policy or other coverage.
We won't let this happen to you.
The licensed Medicare Supplement Policy Agents in our company are also Medicare Supplement Policy Field Underwriters. So that means we'll not only aid to sign up for the least expensive Medicare Supplemental Policies, but we'll make certain that you'll be qualified for the policy before we ask you to apply. By answering some fast questions about your health, you could easily be instantly signed up for a Medigap accepts your application you when you first submit it, sans problems or concerns about of losing coverage.
We will not recommend a Medigap Policy for you until we're certain you will qualify be approved by the Medigap Policy company. Morever, our Best Monthly Premium Guarantee makes certain that you never receive a bill for our professional and that you pay the best price on the policy you qualify for.
To make this easy, we have made arrangements with all of our Medicare Insurance Plan Carriers to be able to let you sign up for a policy over the telephone in a matter of minutes. You will receive the lowest Medigap available, a policy for life and, when you sign up for a Medigap Plan F, you'll never pay another dime again for Medicare-covered Medicare-eligible.


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Niclas Rothman <n...@lechill.com>
|Subqueries / multivalued fields / huge documents
|Fri, 05 Feb 2010 10:40:21 GMT
Hi there, I'm facing a problem that im having difficulties to solve and im wondering if any of you could help on the right way. I have an index storing information about media like videos. Every video object has information about which browsers it is compatible with, e.g. video 1 is compatible with firefox, internet explorer and soforth. An example of such document can be visualized like: <doc> <media> <id>12345</id> <title>A title</title> <description>My description</description> <useragents> <!-- The useragents element can contain up to 15000 entries!!!! --> Mozilla/5.0 (Linux; U; Android 1.5; en-gb; HTC Magic Build/CRB43) AppleWebKit/528.5+ (KHTML, like Gecko) Version/3.1.2 Mobile Safari/525.20.1 Mozilla/4.0 SonyEricssonW910iv/R1CA Browser/NetFront/3.4 Profile/MIDP-2.1 Configuration/CLDC-1.1 UP.Link/220.127.116.11.0 NokiaN81 . . .n </useragents> </media> </doc> Except from finding media objects that are relevant to a users search, e.g. where title equals "A title" I may only serve / display items that are compatible with the users browsers useragent, e.g. find all media objecst that are compatible with Firefox. Problems: 1. One media object can contain up to 15000 useragent entries, can I index this with deccent performance? 2. The useragent values can be "partial" or wildcarded, e.g. "*NokiaN81*" meaning that the media object is compatible with all browsers having a useragent containting "NokiaN81". 3. Can I get any performance out of this, FilterQueries, RangeQueries how? Any help very much appreciated!!!! Regards Magnus
|View raw message


text:
Apoptosis is not the major death mechanism induced by celecoxib on rheumatoid arthritis synovial fibroblasts
DOI: 10.1186/ar2342
© Audo et al.; licensee BioMed Central Ltd. 2007
Received: 11 May 2007
Accepted: 12 December 2007
Published: 12 December 2007
Synovial hyperplasia in rheumatoid arthritis (RA) has been associated with apoptosis deficiency of RA fibroblast-like synoviocytes (FLSs). Celecoxib is a non-steroidal anti-inflammatory drug that has been demonstrated to induce apoptosis in some cellular systems. We have therefore examined the dose- and time-dependent effects of celecoxib on RA FLS viability. Treatment of RA FLSs with celecoxib for 24 hours reduced their viability in a dose-dependent manner. Analysis of celecoxib-treated RA FLSs for their content of apoptotic and necrotic cells by Annexin V staining and TO-PRO-3 uptake displayed only few apoptotic cells. Caspase 3, a key mediator of apoptosis, was not activated in celecoxib-treated RA FLSs, and the presence of specific caspase 3 or pan-caspase inhibitors did not affect celecoxib-induced cell death. Moreover, we could not detect other signs of apoptosis, such as cleavage of poly(ADP-ribose) polymerase, caspase 8 or 9, or DNA fragmentation. We therefore conclude that apoptosis is not the major death pathway in celecoxib-treated RA FLSs.
Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid to prostanoids, which mediate mitogenesis, apoptosis, angiogenesis, blood flow, secondary injury (lipid peroxidation and oxidative stress), and inflammation [1]. The COX-1 isoform is constitutively expressed under physiological conditions, whereas expression of the COX-2 isoform is inducible under pathophysiological, mainly inflammatory, conditions [2]. Consequently, the current pharmacological strategy is to selectively inhibit COX-2 and thereby avoid unfavorable effects of combined COX-1 and COX-2 blocking [1, 2].
Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of joints, leading to a progressive and irreversible joint destruction [3, 4]. The aggressive front of synovial tissue, called pannus, invades and destroys the local articular structure [3, 4]. The pannus is characterized by a synovial hyperplasia that is mainly composed of fibroblast-like synoviocytes (FLSs) combined with a massive infiltration of lymphocytes and macrophages [3, 4]. Increased proliferation and insufficient apoptosis might contribute to the expansion of RA FLSs, and several reports suggest inducing apoptosis of RA FLSs as a therapeutic approach [3, 4].
Celecoxib (4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl] benzenesulfonamide) is an anti-inflammatory drug that specifically inhibits the COX-2. Celecoxib has been described as a pro-apoptotic factor in several human carcinoma cells [5–7]. In addition, it has been reported that high doses of celecoxib have a pro-apoptotic effect on RA FLSs [8]. Here, we report that the cell death induced by high doses of celecoxib on RA FLSs is rapid without displaying characteristics of apoptosis.
Materials and methods
Celecoxib and valdecoxib were generously provided by Pfizer Inc (New York, NY, USA) and dissolved in dimethyl sulfoxide (DMSO) at 100 mM. Indomethacin (Sigma-Aldrich, St Quentin Fallavier, France) was dissolved in ethanol at a final concentration of 100 mM. Pan-caspase inhibitor (z-VAD-fmk [benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone]), caspase 3 inhibitor (z-DEVD-fmk [benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK inhibitor]), and the caspase control inhibitor z-FA-fmk (benzyloxycarbonyl-phenyl-alanyl-fluoromethylketone) specific for cathepsins B and L (R&D Systems, Lille, France) were dissolved at 20 mM in DMSO. Annexin V was purchased from Roche Diagnostic (Meylan, France) and TO-PRO-3 from Invitrogen Corporation (Cergy Pontoise, France). Anti-caspase antibodies were obtained from Cell Signaling Technology (St Quentin Yveline, France), poly(ADP-ribose) polymerase (PARP) antibody from BD Pharmingen (BD Biosciences, Le-Pont-de-Claix, France), and peroxidase-conjugated secondary antibodies were purchased from Sigma-Aldrich.
Preparation of fibroblast-like synoviocytes of patients with rheumatoid arthritis
Fibroblasts were isolated from synovium obtained from patients who met the American College of Rheumatology criteria for RA (revised 1987) and who had undergone surgery for synovectomy or total joint replacement surgery [9]. Fresh synovial tissues were minced and digested in a solution of dispase (Gibco, now part of Invitrogen Corporation) and collagenase (Sigma-Aldrich) and DNase (Calbiochem, now part of EMD Biosciences, Inc., San Diego, CA, USA). Synovial fibroblasts were cultured in RPMI 1640 supplemented with 10% fetal calf serum (FCS). Cells were used at passages 4 to 10, when they constitute a homogeneous population of fibroblasts, free of detectable T cells or macrophages. Upon reaching confluence, the cells were passaged by brief trypsinization. For experimentation, the content of FCS in the media was progressively decreased from 10% to 1% with final starvation for 12 hours in RPMI 1640 media containing 1% FCS, as described previously [10].
Analysis of cell viability and apoptosis
Cell viability was measured by taking metabolic activity as a readout using the Celltiter 96 AQueous cell proliferation (MTS) assay (Promega Corporation, Charbonnières, France) after 24 hours of cell culturing according to the manufacturer's instructions. Apoptotic RA FLSs were identified by resuspending 1 × 105 cells in 100 μL of Annexin V Binding buffer containing 5 μL of Annexin V-fluorescein isothiocyanate (10 μg/mL; R&D Systems) for 15 minutes at room temperature. Upon addition of TO-PRO-3 (1:2,000), cells were analyzed by flow cytometry (FACSCalibur; BD Biosciences) [11].
Cell proliferation assay
Proliferation was evaluated measuring DNA synthesis by incorporation of tritiated [3H]thymidine. FLSs were seeded in 96-well flat-bottom culture plates at a density of 1 × 104 cells per well. Cells were cultured in RPMI 1640 with decreasing concentrations of FCS (10% and 5%) and then synchronized for 24 hours with RPMI 1640 and 1% FCS. FLSs were stimulated for 72 hours. Every condition was tested in quadruplicate. [3H]thymidine (1 μCi/well) was added 24 hours before the end of the assay. FLSs were lysed using a round of freeze-thaw cycle and then transferred onto a membrane filter using Harvester 96 (Tomtec, Hamdem, CT, USA). [3H]thymidine incorporated into DNA was quantified using a scintillation counter 1450 MicroBeta Trilux (Wallac, now part of PerkinElmer Life and Analytical Sciences, Inc., Waltham, MA, USA').
Western blotting analysis
Synovial cells were seeded in six-well plates at 2 × 105 cells per well or in 6-cm dishes at 4 × 105 cells. After serum starvation, RA FLSs were treated as indicated. Both detached and adherents cells were collected, washed twice with cold phosphate-buffered saline (PBS), and treated with lysis buffer as described previously [10]. Total cell extracts were resolved by SDS-PAGE and proceeded for immunoblot analysis as described previously [12]. Primary antibodies were diluted according to the manufacturer's instructions (1:1,000 dilutions for anti-caspase and anti-PARP antibodies and 1:5,000 dilution for anti-β-actin antibody), and nitrocellulose membranes were incubated overnight at 4°C with the primary antibodies (or for 1 hour at room temperature for β-actin). Equal loading was confirmed by β-actin expression.
Ac-DEVD-AMC (caspase 3/7 fluorogenic substrate) protease assay
Synovial fibroblasts were seeded in six-well plates at 2 × 105 cells per well. After serum starvation, RA FLSs were treated with either celcoxib or tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), and caspase 3 activation was measured using the Ac-DEVD-AMC protease assay according to the manufacturer's instructions (BD Biosciences).
DNA fragmentation
FLSs were seeded in 96-well flat-bottom culture plates at 1 × 104 cells per well, cultured with decreasing concentrations of FCS (10%, 5%, and 1%) as described above, and incubated for 12 hours with either celecoxib or TRAIL. Cells were collected, washed with PBS, and processed for quantification of DNA fragments using an enzyme-linked immunosorbent assay according to the manufacturer's instructions (Cell Death Detection ELISAPLUS; Roche Diagnostic).
Celecoxib decreases cell activity and proliferation of rheumatoid arthritis fibroblast-like synoviocytes
The decreased viability of celecoxib-treated RA FLSs was mirrored by their decreased proliferation capacity as measured by thymidine incorporation. Celecoxib inhibited RA FLS proliferation in a dose-dependent manner at all of the concentrations tested (20, 40, 50, and 60 μM), and only marginal thymidine incorporation was detectable in the presence of 60 μM celecoxib (Figure 1b).
Celecoxib-treated rheumatoid arthritis fibroblast-like synoviocytes bypass the state of early apoptosis
No caspase activity is detectable in celecoxib-treated rheumatoid arthritis fibroblast-like synoviocytes
One mechanism that is consistently implicated in apoptosis is the activation of a cascade of cytosolic proteases called caspases. Caspases are synthesized as inactive proenzymes that are processed by proteolytic cleavage to form an active enzyme. A member of this family, caspase 3 (CPP32, apopain, and YAMA), plays a central role in the execution of apoptosis in mammalian cells, and activation of caspase 3 is therefore a hallmark of apoptotic cells [14].
Active caspase 3 proteolytically cleaves and activates, among other targets, PARP involved in DNA repair and DFF40/CAD DNase, the executor of nuclear DNA fragmentation. Apoptotic cells are characterized by cleavage of the native 116-kDa form of PARP into 85-kDa and 25-kDa forms. Concurring with the observed absence of the active form of caspase 3, PARP cleavage was not observed in celecoxib-treated RA FLSs but was observed in those treated with TRAIL (Figure 7c). Moreover, we could not detect DNA fragmentation in RA FLSs treated for either 12 or 24 hours with celecoxib concentrations of 40, 50, or 60 μM, but we could in TRAIL-treated cells (Figure 7d). Taken together, our results suggest that the cell death pathway induced by celecoxib on RA FLSs occurs in a caspase-independent manner.
Apoptosis is a form of cell death in which a programmed sequence of events leads to the elimination of cells without releasing harmful substances into the surrounding area. Apoptosis plays a crucial role in controlling cell numbers by eliminating old cells, unnecessary cells, and unhealthy cells. Deregulation of apoptosis thus can lead to the survival and hyperproliferation of unwanted cells such as FLSs in RA. Therefore, one strategy for treatment is the design of drugs that can restore the normal apoptotic pathways in hyperproliferative cells.
The anti-inflammatory drug celecoxib, an inhibitor of COX-2, was reported by Kusunoki and colleagues [8] to be pro-apoptotic on RA FLSs. In that study, the viability of synovial cells was reduced by celecoxib in a dose-dependent manner similar to our observation. Kusunoki and colleagues observed that celecoxib strongly reduced cell viability of RA FLSs when used at concentrations of at least 30 μM. The authors concluded that celecoxib induces apoptosis in RA FLSs as they observed a strong DNA fragmentation in RA FLSs treated with 30 μM celecoxib.
We compared the characteristics of celecoxib-induced cell death in RA FLSs with those induced by the established pro-apoptotic factor TRAIL by Annexin V staining/TO-PRO-3 uptake. We have previously reported that 4 hours of co-culturing with TRAIL resulted in apoptosis of approximately 30% of RA FLSs as determined by Annexin V staining/TO-PRO-3 uptake. This technique allows investigators to distinguish early apoptotic cells (Annexin+/TO-PRO-3-) from late apoptotic/necrotic cells (Annexin+/TO-PRO-3+) [11]. Annexin V is a Ca2+-dependent phospholipid-binding protein with high affinity for phospatidylserine and can be used as a sensitive probe for the early phase of apoptosis that is characterized by phospatidylserine exposure on the cell membrane. Because of increased permeability, Annexin V binding can also occur during cell necrosis, and uptake of DNA stain TO-PRO-3 is taken as a parameter to distinguish necrotic, and thus permeable, cells [11].
Whereas we observed pre-apoptotic cell death in RA FLSs upon 4 hours of TRAIL treatment, pre-apoptotic cells were hardly detectable in celecoxib-treated cells. We exposed RA FLSs to different concentrations of celecoxib for various incubation times, but under none of the tested conditions were pre-apoptotic (that is, Annexin V+ and TO-PRO-3-) cells detectable. Cell death was observed only at concentrations above 40 μM celecoxib (Figure 2), although we detected a decrease in the metabolic activity of FLSs treated with lower concentrations of celecoxib, which is in agreement with Kusunoki and colleagues [8]. We also confirm that celecoxib strongly inhibited RA FLS proliferation, as shown in their study [8].
It has been suggested that cell death should be classified as apoptosis only if execution of cell death is dependent on caspase activity [14]. We therefore tested whether caspase 3 is activated in celecoxib-treated cells as caspase 3 is a key executor of apoptosis. Whereas the cleaved (thus active) form of caspase 3 was detectable in cell lysates of TRAIL-treated RA FLSs, only the proform of caspase 3 was visible in celecoxib-treated cells. Concurring with this observation, neither a pan-caspase inhibitor nor a specific caspase 3 inhibitor could protect RA FLSs against celecoxib-induced cell death. Similar observations were made with caspase 8 and caspase 9 inhibitors (data not shown). We therefore conclude that celecoxib-induced cell death in RA FLSs is independent of caspases.
These results are in contrast to those of Kusunoki and colleagues [8], who observed DNA fragmentation in RA FLSs treated for 24 hours with celecoxib and an inhibition of this DNA fragmentation by the addition of caspase 3 inhibitors. A possible explanation for these differences might be the different cell systems used. Kusunoki and colleagues used the first two passages of fibroblasts prepared from synovial tissue for their experimentation. We, however, employed RA FLSs between passages 4 and 10 in order to exclude a contamination of other cell types because approximately 30% of the synovium is composed of macrophage-like synoviocytes.
Moreover, evidence is accumulating that taking DNA fragmentation as the readout of caspase inhibition is not sufficient to conclude the contribution of caspases to cell death [15]. It is therefore possible that the caspase dependency of DNA fragmentation described by Kusunoki and colleagues is a secondary event during celecoxib-induced cell death, which could also explain their observed dose-independent effect of the caspase inhibitors. To conclude an apoptotic cell death, other parameters of cell death such as mitochondrial dysfunction, phosphatidylserine exposure, and plasma membrane permeabilization have to be considered [15]. Nevertheless, DNA fragmentation can also be observed in non-apoptotic cell death pathways, as for example during necrosis [16].
We conclude from our results that apoptosis is not the major death pathway in RA FLSs induced by high concentrations of celecoxib. This observation is in line with that of a previous report, in which celecoxib was described as being able to induce non-apoptotic cell death in cardiac myocytes [17]. Administration of antioxidants, such as dithiothreitol, N-acteyl-cysteine, or calcium-blocking reagents, did not alter celecoxib-induced cell death, excluding an implication of oxidative stress and intracellular calcium signals (data not shown). The morphological changes together with the rapid membrane permeabilization of RA FLSs during celecoxib treatment suggest rather a necrotic-like cell death. Necrosis, however, leads to the release of inflammatory cellular contents which is unfavorable in the treatment of RA. The proposed local administration of higher celecoxib concentrations in the synovial space of the joint of arthritis patients [8] must therefore be reconsidered.
= cyclooxygenase
= dimethyl sulfoxide
= fetal calf serum
= fibroblast-like synoviocyte
= poly(ADP-ribose) polymerase
= phosphate-buffered saline
= rheumatoid arthritis
= tumor necrosis factor-related apoptosis-inducing ligand
- z-DEVD-fmk:
= benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK inhibitor
- z-VAD-fmk:
= benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone.
This work was supported by Société Française de Rhumatologie and Pfizer Laboratory (New York, NY, USA). We thank Lourdes Planelles for help with the fluorescence-activated cell sorting analysis, Solange Desagher and Nelly Noraz for critical reading of the manuscript, and Michel Chammas and Bertrand Coulet for providing synovial tissues.
Authors' Affiliations
- Williams C, Mann M, DuBois R: The role of cyclooxygenases in inflammation, cancer, and development. Oncogene. 1999, 18: 7908-7916. 10.1038/sj.onc.1203286.View ArticlePubMedGoogle Scholar
- Crofford L: COX-1 and COX-2 tissue expression: implications and predictions. J Rheumatol Suppl. 1997, 49: 15-19.PubMedGoogle Scholar
- Pope RM: Apoptosis as a therapeutic tool in rheumatoid arthritis. Nat Rev Immunol. 2002, 2: 527-535. 10.1038/nri846.View ArticlePubMedGoogle Scholar
- Firestein GS: Evolving concepts of rheumatoid arthritis. Nature. 2003, 423: 356-361. 10.1038/nature01661.View ArticlePubMedGoogle Scholar
- Maier TJ, Schilling K, Schmidt R, Geisslinger G, Grosch S: Cyclooxygenase-2 (COX-2)-dependent and -independent anticarcinogenic effects of celecoxib in human colon carcinoma cells. Biochem Pharmacol. 2004, 67: 1469-1478. 10.1016/j.bcp.2003.12.014.View ArticlePubMedGoogle Scholar
- Liu X, Yue P, Zhou Z, Khuri FR, Sun SY: Death receptor regulation and celecoxib-induced apoptosis in human lung cancer cells. J Natl Cancer Inst. 2004, 96: 1769-1780.View ArticlePubMedGoogle Scholar
- Jendrossek V, Handrick R, Belka C: Celecoxib activates a novel mitochondrial apoptosis signaling pathway. FASEB J. 2003, 17: 1547-1549.PubMedGoogle Scholar
- Kusunoki N, Yamazaki R, Kawai S: Induction of apoptosis in rheumatoid synovial fibroblasts by celecoxib, but not by other selective cyclooxygenase 2 inhibitors. Arthritis Rheum. 2002, 46: 3159-3167. 10.1002/art.10692.View ArticlePubMedGoogle Scholar
- Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS, et al: The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988, 31: 315-324. 10.1002/art.1780310302.View ArticlePubMedGoogle Scholar
- Morel J, Audo R, Hahne M, Combe B: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt. J Biol Chem. 2005, 280: 15709-15718. 10.1074/jbc.M414469200.View ArticlePubMedGoogle Scholar
- Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995, 184: 39-51. 10.1016/0022-1759(95)00072-I.View ArticlePubMedGoogle Scholar
- Morel JC, Park CC, Zhu K, Kumar P, Ruth JH, Koch AE: Signal transduction pathways involved in rheumatoid arthritis synovial fibroblast interleukin-18-induced vascular cell adhesion molecule-1 expression. J Biol Chem. 2002, 277: 34679-34691. 10.1074/jbc.M206337200.View ArticlePubMedGoogle Scholar
- Posmantur R, McGinnis K, Nadimpalli R, Gilbertsen RB, Wang KK: Characterization of CPP32-like protease activity following apoptotic challenge in SH-SY5Y neuroblastoma cells. J Neurochem. 1997, 68: 2328-2337.View ArticlePubMedGoogle Scholar
- Leist M, Jaattela M: Four deaths and a funeral: from caspases to alternative mechanisms. Nat Rev Mol Cell Biol. 2001, 2: 589-598. 10.1038/35085008.View ArticlePubMedGoogle Scholar
- Kroemer G, Martin SJ: Caspase-independent cell death. Nat Med. 2005, 11: 725-730. 10.1038/nm1263.View ArticlePubMedGoogle Scholar
- Dong Z, Saikumar P, Weinberg JM, Venkatachalam MA: Internucleosomal DNA cleavage triggered by plasma membrane damage during necrotic cell death. Involvement of serine but not cysteine proteases. Am J Pathol. 1997, 151: 1205-1213.PubMed CentralPubMedGoogle Scholar
- Hasinoff BB, Patel D, Wu X: The cytotoxicity of celecoxib towards cardiac myocytes is cyclooxygenase-2 independent. Cardiovasc Toxicol. 2007, 7: 19-27. 10.1007/s12012-007-0002-8.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
The TSA announced the launch of its "trusted traveler" program on Tuesday, which they announced back in May.
This includes a "PreCheck" program which is an attempt by the TSA to distance themselves from a "one-size-fits-all approach," to a more risk-based one, as TSA Administrator John Pistole said in a press release. Willing travelers will provide more personal information at TSA checkpoints, in an attempt to focus the TSA's efforts on more risk-based travelers.
As Pistole said in the press release:
By learning more about travelers through information they voluntarily provide, and combining that information with our other layers of security, we can focus more resources on higher-risk and unknown passengers. This new screening system holds great potential to strengthen security while significantly enhancing the travel experience whenever possible for passengers.
During this pilot, TSA will use pre-screening capabilities to make intelligence-based risk assessments on passengers who voluntarily participate in the TSA PreCheck program and are flying domestically from one of the four pilot sites: Hartsfield-Jackson Atlanta International, Detroit Metropolitan Wayne County, Dallas/Fort Worth International and Miami International airports.
So who fits the bill? Participants include certain frequent fliers from American and Delta airlines as well as member of the Customs and Border Protection's Trusted Traveler programs. Passengers will go through expedited screening, the TSA stated. Here's the catch: Delta passenger participants must be flying out of Atlanta and Detroit and American Airlines passengers must be flying out of Miami and Dallas airports to participate, CNN reports.


text:
Economists have big concerns about competition law 'effects test' proposal
Former head of the competition watchdog and Monash University Business and Economics Faculty Professor, Graeme Samuel, and Frontier Economics chairman, Philip Williams tell Lucy Barbour why they don't agree with calls for the introduction of an 'effects test' provision in competition law. The National Farmers' Federation has called for a 'hybrid' effects test in its submission to the Government's Competition Policy Review. The NFF's general policy manager, Tony Maher, says it would be a step towards creating a more transparent and balanced supply chain and fairer marketplace. Read more.
Source: ABC News | Duration: 4min 13sec
Topics: rural, agribusiness, business-economics-and-finance, industry, markets, advertising-and-marketing, agricultural-marketing, laws, law-crime-and-justice, retail, small-business, canberra-2600


text:
Massage Therapy
What to expect from us?
Step 1: Meet & Greet
Once you arrive at Grismby Massage Therapy Clinic, a therapist will greet you and ask you to fill out a confidential health history form. (Please come 5 – 10 minutes early to ensure you have time to fill out the form.) This Form is where you record any medical history, past/present injuries and other physical conditions. Your Registered Massage Therapist (RMT) will inquire about the reason(s) you are coming for a massage, your current physical condition and any specific areas in which you are experiencing pain or would like addressed during your massage.
Your Treatment Room
Your RMT will then take you to a peaceful, spa-like treatment room where a comfortable massage table fitted with a warming blanket and clean sheets awaits. There is a small shelf and chair for your personal belongings. Your RMT will leave the room to wash their hands and wait outside, while you disrobe to your level of comfort. Some clients prefer to completely disrobe, while some will remain partially clothed. It is completely up to you. Once comfortable, you will lie on the massage table, under the top sheet. Your RMT will give you a few minutes for this process and will knock on the door to ask if you are ready before entering your room.
Your comfort and privacy is important to us. Your massage therapist will only uncover the part of the body they are working on, ensuring that your modesty is respected at all times.
You can expect a tranquil and comfortable environment during your massage.
Your Needs
Before beginning the session, your therapist will ask you to alert them if, at any time during the session, a technique or stroke they are using is uncomfortable. Depending on your specific needs or requests, your massage therapist will either perform a customized full-body massage (for general relaxation and stress reduction) or focus on the specific parts of your body that you have requested. Our massage therapists have a variety of oils and lotions that are hypoallergenic for you to choose from for your treatment.
To Paradise and Back
Once your massage therapy session is completed, your therapist will leave the room so you can re-dress.
We look forward to seeing you on your next visit, and any questions you may have will gladly be answered. And please, if no one is available to take your call please leave a message and we will get back to you shortly.
Types of Massage Therapy Offered
Pregnancy Massage
Perfect for relieving those aches and pains of being pregnant!
- Increases blood circulation which can reduce swelling
- Provides relief from muscle cramps, spasms and myofascial pain
- Improves outcome of labour and eases labour pain
- Enhances the pliability of skin and underlying tissues
Deep Tissue Massage
Focuses on the muscles located below the surface of the top muscles. It is often recommended for people who experience consistent pain, are involved in heavy physical activity and who have sustained physical injury.
- Reduces chronic pain
- Improves blood pressure
- Rehabilitates injured muscles
Sports Massage
Perfect for athletes whose bodies are under stress due to physical activity.
- Prepare your body for peak athletic performance
- Relieves swelling
- Reduces muscle tension
- Promotes flexibility to prevent injuries
Couples/Girlfriends Massage
Perfect for some relaxation with your significant other or friends!
College of Massage Therapists of Ontario Standards of Practice.
What are Standards of Practice?
The College of Massage Therapists of Ontario has developed the Standards of Practice for Massage Therapists. Through the development and use of these Standards the College of Massage Therapists continues to display its commitment to the protection of the public, serving its members, and promoting the highest possible quality of massage therapy practice in a safe and ethical manner.
The Standards of Practice are intended to be generic and have been developed to describe the outcomes of the various tasks the therapist is required to perform within the Scope of Practice. The Standards of Practice describe how well a therapist is expected to perform. How to do each task will be determined by the curriculum developed by the educational programs.
The Standards of Practice form a live and dynamic document that will evolve as changes in practice evolve.
How Can Massage Be Medically Beneficial?
Massage Therapy helps you relax, re-align and rejuvenate. There are many positive aspects to receiving Massage Therapy on an ongoing basis, and with the busy lives we lead, we can all benefit from a little stress-management. The experienced, Registered Massage Therapists at Grimsby Massage Therapy Clinic provide exceptional care and will design an effective customized massage treatment to address your individual needs.
More Canadians are turning to therapeutic massage treatment to provide relaxation, relieve tension associated with the daily stresses of their busy lives, or simply to help maintain good health and achieve a balanced lifestyle.
Oh, and let's not forget how great it feels to be treated by a Registered Massage Therapist.
There are so many healthy benefits to receiving Massage Therapy on a regular basis:
- Relieves stress
- Encourages relaxation
- Improves posture
- May lower blood pressure
- Improves circulation
- Helps manages pain naturally
- Decreases muscle pain
- Helps improves flexibility and ranges of motion
- Helps relieve tension- related headaches
- May help post operative rehabilitation
- Eases pregnancy aches and pains
- Improves rehabilitation after injury
Massage Therapy in Ontario is a growing profession, that is part of the health care team, providing primary care to clients and to the general population concerned with maintaining and enhancing health and well being. Massage therapists use Swedish massage techniques and additional forms of soft tissue manipulation and joint mobilisations as a complement to existing courses of medical treatment or as an exclusive treatment programme.
Massage Therapists treat many conditions such as muscular "weakness" and "wasting" arising from disease, injury or immobility resulting from:
- Sprains and strains
- Fractures
- Trauma (i.e., motor vehicle accidents)
- Sports/athletic injuries
- Tendonitis
- Muscle spasms or spasticity
- Post operative care and rehabilitation
Massage therapists also treat chronic conditions resulting in musculo-skeletal involvement.
For example:
- Muscular tension headaches
- Chronic nonspecific low back pain
- "Stress" syndromes
- Chronic pain syndromes
- Muscular pain involvement in arthritis
- Muscular tension of the jaw, face and neck
- Cupping Therapy
- Indie Head Massage
- Massage Therapy
- Osteopathy
- Pregnancy Massage
- Reflexology
- Acupuncture
Recent News:
- Welcome Madelyne to our team!
Grimsby Massage Therapy Clinic would like to officially welcome R.M.T Madelyne Maingot to our team! Madelyne is an awesome addition to our already incredible team of massage therapists and practitioners. Book an appointment with Madelyne today by visiting our website or calling the clinic at 905-309-8694.
- Welcome RMT Valerie Eder to Grimsby Massage!!
Grimsby Massage gladly welcomes Valerie Eder, RMT to our clinic! Valerie is an awesome addition to our already incredible team of massage therapists and practitioners. She has a strong background in sports related injuries and athletics. Throughout the years she has had the opportunity to work with a wide range of populations including weekend warriors, […]
- Welcome Yanan and Samantha!
Grimsby Massage Therapy Clinic would like to officially welcome both Yanan Mou and Samantha Dunn! These two incredible ladies have just recently joined our team and we are beyond thrilled to be working alongside them. With a true passion in massage, these lovely ladies will devote their time and strength into your sore, tired muscles – […]


text:
Movie Summary
The Grace Card
Web Site: http://www.gracecardmovie.com/
Description: Everything can change in an instant ... and take a lifetime to unravel. When Mac McDonald loses his son in an accident, years of bitterness and pain erode his love for his family and leave him angry with God ... and everyone else. Can Mac and his new patrol partner, Sgt. Sam Wright, somehow join forces to help one another when it's impossible to look past their differences -- especially the most obvious one? Every day, we have the opportunity to rebuild relationships and heal wounds by extending and receiving God's grace. Offer "The Grace Card" ... and never underestimate the power of God's love.
Movie summaries and listings powered by Cinema-Source
Related Content on Movies from Infoplease:


text:
Blue Mountains Fuel Injection
Car Repair, Automobile Repair, Automotive Parts
Harry & Brad have been involved with Fuel Catalysts for almost 18 years, originally with Fuelstar and due to the early results and the benefits shown, the better design and subsequent improvements has led to them marketing the FuelMate Catalyst developed by Fuel Dynamics, here in Sydney.
What can you expect from a Fuelmate Fuel Catalyst:-
-Improved fuel economy
-More power
-Increased torque
-Prolonged engine life
-Lowers Toxic Exhaust emissions
-Converts leaded engines to run on unleaded fuel
(no pinging)
-5 year / 500,000 kms Manufacturers Warranty
-6 Month Money Back Guarantee
-Suits Petrol, diesel & LPG
For more information visit our website: http://www.bmfi.com.au/index.html
Or call us today on: 02 6351 3666
Print this page
Come in and let Brad advise you about your car, and....
"Deal with Mechanics that really love what they Do"
Dyno Tuning and Performance work made easy with the experts at Blue Mountains Fuel Injection!
Inter-Ject is the best Chemical in the world... It's what our customers say... and they love it!... (and it's because it works!)
Create your free Hotfrog business profile
Follow Hotfrog on:
Share on:
Hotfrog Small Business Hub


text:
The First Minister tells the Chief Constable that he must address what he calls a perception in the unionist community that they treat loyalists differently than republicans.
Belfast City Council want to light up City Hall on the Twelfth, St Patrick's Day and during Gay Pride but not on the Queen's Birthday or Remembrance Day.
Should office workers be allowed to keep a pillow under their desks so they can get a power nap while at work?
And apparently nearly a quarter of men in Northern Ireland don't change their underwear daily, and one in six have worn their partner's pants.


text:
Infrared and Raman Spectra of Inorganic and Coordination Compounds
Pt. A 6th Revised edition
By (author) Kazuo Nakamoto
Format: Hardback
Normal Price: $221.95
Your Price: $199.76 AUD, inc. GST
Shipping: $7.95 per order
You Save: $22.19! (10% off normal price)
Plus...earn $9.99 in Boomerang Bucks
Availability: Available to Backorder, No Due Date for Supply
Infrared and Raman Spectra of Inorganic and Coordination Compounds by Kazuo Nakamoto
Book DescriptionThe Sixth Edition of this classic work comprises the most comprehensive and current guide to infrared and Raman spectra of inorganic, organometallic, bioinorganic, and coordination compounds. From fundamental theories of vibrational spectroscopy to applications in a variety of compound types, this has been extensively updated. New topics include the theoretical calculations of vibrational frequencies (DFT method), chemical synthesis by matrix co-condensation reactions, time-resolved Raman spectroscopy, and more. This volume is a core reference for chemists and medical professionals working with infrared or Raman spectroscopies and an excellent textbook for graduate courses.
Buy Infrared and Raman Spectra of Inorganic and Coordination Compounds book by Kazuo Nakamoto from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780471743392
(234mm x 162mm x 24mm)
Pages: 432
Imprint: Wiley-Blackwell (an imprint of John Wiley & Sons Ltd)
Publisher: John Wiley and Sons Ltd
Publish Date: 27-Jan-2009
Country of Publication: United Kingdom
Other Editions...
» View all editions of Infrared and Raman Spectra of Inorganic and Coordination Compounds...
Books By Author Kazuo Nakamoto
Infrared and Raman Spectra of Inorganic and Coordination Compounds, Hardback (February 2009)
The 6th edition of this classic comprises the most comprehensive guide to infrared and Raman spectra of inorganic, organometallic, bioinorganic, and coordination compounds. From fundamental theories of vibrational spectroscopy to applications in a variety of compound types, it is extensively updated.
Drug-DNA Interactions, Hardback (July 2007)» View all books by Kazuo Nakamoto
Drug-DNA Interactions: Structures and Spectra describes the types of structural and bonding information that can be obtained by using specific physico-chemical methods. Structural and spectroscopic studies of drug-DNA adducts are classified first by bonding types and then by structure type of the drug.
» Have you read this book? We'd like to know what you think about it - write a review about Infrared and Raman Spectra of Inorganic and Coordination Compounds book by Kazuo Nakamoto and you'll earn 50c in Boomerang Bucks loyalty dollars (you must be a member - it's free to sign up!)
Author Biography - Kazuo Nakamoto
Kazuo Nakamoto, PHD, received his DSc from Osaka University in 1953 and stayed on as a faculty member until 1957. In 1958, he joined the faculty at Clark University, moved to Illinois Institute of Technology in 1961, and then to Marquette University in 1969, where he became the university's first Wehr Professor of Chemistry. Dr. Nakamoto has directed the research of more than eighty-five graduate students and postdoctoral associates, and has published over 225 research papers, twenty review articles, and twelve books.
Facebook Page | Twitter | Google+ | Bulletin e-Newsletter | Blog | RSS
Become a Member | Book Lists | Recently Released | Coming Soon | Fast Delivery Books
Bestselling Books: Our Current Bestsellers | Australia's Hottest 1000 Books | Bestselling Fiction | Bestselling Crime Mysteries and Thrillers | Bestselling Non Fiction Books | Bestselling Sport Books | Bestselling Gardening and Handicrafts Books | Bestselling Biographies | Bestselling Food and Drink | Bestselling History | Bestselling Travel Books | Bestselling School Textbooks & Study Guides | Bestselling Children's General Non-Fiction | Bestselling Young Adult Fiction | Bestselling Children's Fiction | Bestselling Picture Books | Top 100 US Bestsellers
For: Schools | Organisations | Libraries | Publishers | Authors | Book Clubs | Bloggers | Affiliates
About Us | Contact Us | Help Desk | Security | Privacy | Terms of Use | Copyright
Phone: 1300 36 33 32 (9am-5pm Mon-Fri AEST) - International: +61 2 9960 7998 - Online Form
Address: Boomerang Books, 878 Military Road, Mosman Junction, NSW, 2088
© 2003-2017. All Rights Reserved. Eclipse Commerce Pty Ltd - ACN: 122 110 687 - ABN: 49 122 110 687
For every $20 you spend on books, you will receive $1 in Boomerang Bucks loyalty dollars. You can use your Boomerang Bucks as a credit towards a future purchase from Boomerang Books. Note that you must be a Member (free to sign up) and that conditions do apply.


text:
Browse by Creator
Group by: Item Type | No Grouping | Creators | Year of publication, submission, or completion
Jump to: Book Section | Conference or Workshop Item
Number of items: 4.
Book Section
Sunitiyoso, Y., Avineri, E. and Chatterjee, K. (2010) Complexity and travel behaviour: A multi-agent simulation for investigating the influence of social aspects on travellers' compliance with a demand management measure. In: Silva, E. and de Roo, G., eds. (2010) A Planners Encounter with Complexity. Aldershot: Ashgate, pp. 209-226. ISBN 978-1-4094-0265-7 Available from: http://eprints.uwe.ac.uk/10717
Sunitiyoso, Y., Avineri, E. and Chatterjee, K. (2009) Role of minority influence on the diffusion of compliance with a demand management measure. In: Kitamura, R., Yoshii, T. and Yamamoto, T., eds. (2009) The Expanding Sphere of Travel Behaviour Research. Emerald Group Publishing Limited, pp. 643-672. ISBN 978-1-84855-936-3 Available from: http://eprints.uwe.ac.uk/10714
Conference or Workshop Item
Sunitiyoso, Y., Avineri, E. and Chatterjee, K. (2010) Applying an experimental economics tool to understand the role of social interaction in social dilemma situations. In: Pan-Pacific Conference XXVII: Managing and Competing in the New World Economic Order, Bali, Indonesia, May - June, 2010. Available from: http://eprints.uwe.ac.uk/13100
Sunitiyoso, Y., Avineri, E. and Chatterjee, K. (2007) The role of social interactions in travel behaviour: Designing experimental tools to explore behavioural assumptions. In: Frontiers in Transportation 2007: Social Interactions, Amsterdam, The Netherlands, October, 2007. Available from: http://eprints.uwe.ac.uk/9773
This list was generated on Mon Feb 27 02:26:18 2017 GMT.


text:
Arsenal manager Arsene Wenger has his sights set on securing Champions League football at West Brom on Sunday.
The Gunners went into the final game of the 2005/2006 campaign behind rivals Tottenham in fifth place and needing a favour from West Ham.
Spurs eventually lost 2-1 at Upton Park, while the Gunners beat Wigan in what was their final match at Highbury.
Afterwards, it was claimed by then Tottenham manager Martin Jol a bug caused by food poisoning at the team's hotel had depleted his squad's performance on the day.
An investigation by the Health Protection Agency ruled the pre-match meal was not the cause of the virus, with tests on the players showing one had a form of gastroenteritis which may have spread to the others affected.
Wenger, though, feels there is no need to dwell on the past as his team look to serve up a vintage display at The Hawthorns, which would make whatever Spurs produce against Fulham, now managed by Jol, irrelevant.
"I don't know why they should come out with that if it is not true. It can happen that you have a problem like that," Wenger said.
"They were at West Ham and we were quite down against Wigan - we were 1-0 down."
Wenger joked: "I didn't cook that day. If I had cooked, then more of them would have been ill!"
The Gunners boss added: "We will eat here [on Saturday night]."
Arsenal announced yesterday long-serving number two Rice, 63, will leave his role after 44 years with the club.
Former centre-back Steve Bould is to be promoted from his current role as youth team coach, which for Wenger will make it a seamless transition as the club moves forward.
"One coach doesn't make a new era. It is the team and the players," he said.
"What is important is that we make all the things right. Good players on the pitch make a good team."
Wenger has challenged Arsenal to play the game and not the occasion when they head to West Brom, out to cement their place in the Champions League next season.
The Gunners would have already been assured of third place in the Barclays Premier League had they managed to beat Norwich last Saturday, when the thrilling 3-3 draw did not prove too costly as other results went their way on Sunday.
With a slender one-point lead over Spurs and two from Newcastle, there is little margin for further error by a team which has drawn the last three games.
Throw in the fact Sunday will be Roy Hodgson's final game in charge at The Hawthorns before he takes over at England, and everything is set for a fascinating encounter.
Wenger, though, wants to keep things simple as he targets victory.
"We have a massive game in front of us, but I feel we are focused, switched on. I am confident we will do well," said the Arsenal boss, who has guided the club into the Champions League for the last 14 seasons.
"For us it is just to find the right mixture - to be focused, combative and relaxed, and especially be faithful to play our football like we want to.
"At the end of the day it will be down to playing well, no matter what is at stake."
Arsenal could have Theo Walcott back from a hamstring problem at The Hawthorns, with the England winger set to rejoin training today.
- More about:
- Arsenal
- Premier League
- Tottenham Hotspur
- UEFA Champions League


text:
Talking points
Our editors will be looking ahead today to these developing stories ...
Related topics
Figures that could alter US election
The most anticipated economic figures still to be released before the United States presidential election are out tonight: October's unemployment data. The figures are expected to draw close scrutiny after an unexpected fall in September's unemployment rate to 7.8 per cent - the lowest since Barack Obama took office - prompted accusations from leading business figures that the data had been massaged to help the president's re-election campaign. With the presidential election just days away, the jobless figures could factor into people's voting intentions.
Cathay heads back to the Big Apple
Cathay Pacific flights from Hong Kong to John F. Kennedy Airport resume following the disruption of Hurricane Sandy, according to an airline statement. Cathay cautioned travellers to expect "considerable damage" and limited services at the airport. The huge storm, which wreaked havoc along the US east coast, resulted in the cancellation of 12,000 flights.
First bite of the mini Apple
Less than two weeks after its very existence became known to the general public, the iPad mini is being launched in Hong Kong. The world's biggest technology company introduces its compact version of the Apple iPad in some 34 markets today. It will also release the fourth-generation iPad, which was unveiled last month.
Hopes for early heat as temperatures plummet
Some mainland cities are hoping their public heating systems may be turned on ahead of schedule after the China Meteorological Administration gives its monthly report in Beijing today. China has been suffering from a series of cold fronts that have brought temperatures down by up to 14 degrees. Temperatures in the capital are expected to fall below zero next week.
Carrie Lam tackles leadership change
Chief Executive Leung Chun-ying may want to take a few notes as Chief Secretary Carrie Lam Cheng Yuet-ngor delivers a lecture entitled "Leadership - Change and Innovation" at the Chinese University of Hong Kong. Lam, who graduated from the University of Hong Kong in 1980 with a degree in social science, has been invited to speak as part of a programme at Shaw College designed to broaden students' experience.
Debate resumes on finance committee rules
Lawmakers resume debate on the Beijing-loyalist camp's attempt to tighten the finance committee's procedures to prevent filibustering. Ip Kwok-him, of the Democratic Alliance for the Betterment and Progress of Hong Kong, proposed that members should be allowed to table just one motion per debate without prior notice. His suggestion followed last summer's marathon filibustering tactics by radical lawmakers. However, finance committee members will first have to vote on rules for tabling motions to amend procedures.
Most Popular
- 1
- 2
- 3
- 4
- 5


text:
Of all your B2B marketing efforts, e-mail seemingly has the least environmental impact.
But sending all those messages electronically hasn't led to a paperless office. The more we use computers and printers, the more paper and energy we consume. How many times each day do you print an e-mail to read later offline or to keep a paper copy "just in case?"
Most people assume global warming is caused by burning oil and gas. In fact, between 25 and 30 percent of the greenhouse gases released into the atmosphere each year — 1.6 billion tons — are caused by deforestation.
When you add the gas and oil we use by running computers to read e-mail — and to check it constantly on our BlackBerry devices — you can see that e-mail isn't all that environmentally benign.
With Earth Day coming up on Sunday, here are some ways to make your e-mail a little greener.
- Print less with GreenPrint: When you print e-mail and Web pages, there's usually one useless sheet at the end, the one with the unsubscribe message, your e-mail provider's logo, legal disclaimers, etc.
You can eliminate printing unnecessary pages by installing GreenPrint. The software previews the document and highlights potentially unnecessary pages for removal. If you agree a page isn't needed, it won't be printed. It works with Word or any other program.
Walter Mossberg favorably reviewed GreenPrint in the "Wall Street Journal,", and Gartner named it a Cool Vendor, quite a coup for a new company.
GreenPrint provides reports on how much ink, paper, and money you save. In the few months I've used GreenPrint, I saved 127 sheets of paper (worth $3.17), and $4.45 worth of ink, according to the report. Not a huge savings, but for large users, the amount can be substantial.
- Unplug your BlackBerry: Checking e-mail from a BlackBerry seems environmentally neutral, as no paper's involved. But did you know chargers for BlackBerry, cell phone, and handheld games can consume 10 percent of the electricity in your house?
I discovered this while working on the Polar Bear S.O.S. campaign for the Natural Resources Defense Council. Polar bears were one of the first major species to be victims of global warming. In reading about their plight and wondering what the average person could do, a light bulb went off in my head (compact fluorescent, of course!). The idea is stickers to put on your chargers with the words, "Unplug for Polar Bears." You can order these free stickers online. While there, consider making a donation!
- Recycle old electronics: Consumer electronics products contain thousands of substances, some of which are hazardous to human and environmental health. If old equipment isn't properly recycled, they can seep into air, soil, and water. Everyone should make an effort to learn more about how to recycle her devices.
And if you happen to live near my hometown of Brooklyn, you can recycle your electronics this Saturday, April 21 at the Park Slope Eco Fair. I'm not ready to recycle my BlackBerry yet, but I just packed up an old color printer to bring over. Perhaps I'll see you there.
Know of other ways to make B2B e-mail practices greener? Send them to Karen.
Karen is off this week. Today's column ran earlier on ClickZ.
Related reading
A wish for email in 2017
There are so many ways in which email continues to develop and progress, but in one way email still lives in the last decade.
What is email marketing and how do you create a successful strategy?
Email marketing may not be new, but it's still effective, so now is the time to dive into the best ways of mastering it to improve marketing success.
What did we learn from the 2016 US election? Not what you think
As the United States makes way for a new resident in the White House, I've been thinking about the election that led up to it. Others have pontificated about the impact email had on the presidential campaigns, but I'm not buying any of it.
A motivational message for retail email marketers (and everyone else, too)
With Halloween, the US presidential election and Thanksgiving in the rear-view mirror, we're now headlong into the all-holiday-all-the-time stream. And, we all know what's coming.


text:
With Instructables you can share what you make with the world, and tap into an ever-growing community of creative experts.
These are great pranks that will for sure make you laugh but not the victim.
First, you will need duct tape matching your sink's color, a sink with a sprayer, and glad wrap. Okay, so make sure everyone in your house is ...
You will need: - A quarter -A busy sidewalk -A toaster(optional) -A paper clip(optional) -Epoxy or some kind of crazy strong glue First, go to your sidewalk ...
April fools pranksby napalm008
APRIL FOOLS! Sometimes the simplest things are the best...Now you might wanna change ...by andrewlangham
sink sprayer squirt gun prankby openroad12
Join 2 million + to receive instant DIY inspiration in your inbox.
© 2016 Autodesk, Inc.


text:
We've set your shipping destination to:
Prices vary based on delivery destination (it's a tax thing), so please change it if you're not shipping within United States as it might affect the price! (Why?)
A powerful, high-proof cask strength Connemara. This is a peated Irish whiskey distilled at Cooley.
Nose: Subtle and soft, quite farmyardy, with nutty notes, blossom and peat.
Palate: Full, punchy and sweet with vanilla spice, smoke, cereals and peat.
Finish: Long, powerful and hot.
Please enter your details to make it easier for us to help you further.
The legal stuff: not providing your contact details may mean we have to delete your
comments if another member of our community complains about them. Whatever happens though, we'll never share
your contact details unless you specifically tell us we can.
Please click here for more details.
Intense turf flavour from Cooley. Connemara enjoyed @ cask strenght, could it get any better one might ask? And the answer is simply, No! For all you who enjoy Islay whisky this one you must buy straigh away.I promise you wont be disappointed. Bar the Turf Mor this is the best whisky I have ever tasted from Ireland./mike-stockholm-sweden
22nd August 2013
Yet another great whiskey from Cooley. This one is almost as good as the Turf Mor. Here we have irish peat at its best. You wont regret buying this one.
29th May 2013
A taste sensation if you like peaty whiskey! Not what you'd expect from a irish whiskey.
6th August 2012
...and I mean that in the best possible way.
14th April 2011


text:
As Banner Health's employed physician group with more than 1,300 physicians and advanced practitioners across more than 65 specialities, BMG, is transforming the delivery of care.
Actively seeking a pediatric intensivist to provide care for critically ill children and ward care.
Florida Pediatric Critical Care, P.A. is team of dedicated and highly qualified practitioners who care exclusively for children in the hospital setting.
Chief of Pediatric Critical Care Medicine - Cohen Children's Medical Center - NYC Suburbs
Seeking a full-Time Director, Peds Ambulatory Care at the Elmhurst Hospital
Thousands of new jobs posted every day. Let us bring them to you. Sign up for alerts.


text:
After a long run that took his daughter from obscurity to Destiny's Child to solo fame, Mathew Knowles will no longer serve as his daughter, Beyonce's, manager.
"I am grateful for everything he has taught me," Beyonce said in a statement to People. "I grew up watching both he and my mother manage and own their own businesses. They were hardworking entrepreneurs and I will continue to follow in their footsteps."
"He is my father for life and I love my dad dearly," she continued. "I am grateful for everything he has taught me."
In 2009, Mathew and Beyonce's mother, Tina Knowles, filed for divorce after reports that he had fathered a child with a woman outside the marriage. At the time, according to the New York Post, Beyonce was not speaking to her father as of June 2010.
In a separate statement released on Monday (March 28), Matthew Knowles acknowledged that the decision for him to step out of Beyonce's career was mutual.


text:
As the leading consumer advocacy website, our technology is being continually upgraded to handle the volume of searches from consumers, the media, the authorities and millions of others from around the world. While we are always in the process of upgrading, our search results may not return all reports. This is only temporary and intermittent. If you are an attorney helping victims, the media, or law-enforcement, please contact us to have us run a complete database search to help your case or story.
|Specific Search
|General Search
|Title City, State
1, Report #881448
Dec 21 2012
|720Funding.com 720 Funding, Khann Inc Scammers, criminals, theives Internet, Internet Call Me so we can discuss! Author: Miami, Florida Credit & Debt Services: 720Funding.com Internet
Advertisers above have met our
strict standards for business conduct.


text:
Using the "offers from" options you can filter merchants from regions which are most common in our database: the UK, Germany, Austria and Poland.
If a checkbox near a flag is selected, offers from merchants from that particular region will be displayed, otherwise they will not be visible.
If you select offers from "all regions", all offers from all merchants will be displayed. These incude offers from merchants in countries not listed here.
Battery: 4.8V, 0.6Ah, NiCd Idle speed: 200rpm Tool fixture: 1/4" bitholder Special features: articulated joint, forward/reverse operation, LED work ...


text:
This much is true.
On 25 May 1928, returning from her third expedition to the North Pole, the airship Italia crashed on the Arctic icepack, killing one crew member on impact and stranding nine more. The remaining six members of the crew were carried off in the envelope of the airship, never to be found. An international rescue effort ensued, but because of apathy and political considerations on the part of the Italian Fascist government, it took more than 49 days to find the survivors.
For whatever reasons, the crash of the Italia has become a footnote in history. Truth to tell, I was completely unfamiliar with the incident before happening, quite by happenstance, upon Ben Towle's quietly brilliant graphic novel Midnight Sun. This is a work firmly entrenched with what makes historical fiction work when done properly, using the details of the event as a backdrop to tell a tale that touches the human condition.
History means precious little until it's placed in the context of how its participants view it in their own lives. In Midnight Sun, we view the crash through the eyes of a reporter only identified as "A. J.", an alcoholic whose last chance at redemption is covering the final fate of the Italia survivors. His paper books him passage aboard the Russian icebreaker Kasspin, headed to rescue the Italia survivors. Meanwhile, the survivors of the Italia grapple with survival on an ice flow moving inexorably north.
The ice flow is a metaphor for the story, to be sure—it represents salvation and death on several levels. And it works admirably, due in no small part to Towle's storytelling gifts. What really hooked me on Midnight Sun, though, was the way he illustrated the story. His style is stark, simple, a bit reminiscent of Herge, but with atmospheric details that call to mind the detail work of Eisner. Towles refers to himself as a cartoonist—not an artist—and that modest attitude about himself leaves him free to concentrate on telling his story.
At its heart, Midnight Sun isn't about a desperate rescue mission, or about coming to grips with one's mortality or even about a final shot at redemption. It's more about how events, seemingly unrelated, converge and influence each other, making for larger, equally unrelated events. Towle paints the story in stark lines, accentuated by minimal gray washes, creating an effect that reflects both the growing desperation of the stranded explorers and the edgy boredom of the rescuers and the reporter.
Midnight Sun isn't a bombastic graphic novel—in fact, I'd liken it more to a cinema verite than a graphic novel. In the confines of a 6.5"X5.5" format and a mere 136 pages, Ben Towle has managed to bring a historical environ to life. His ear for dialogue and his eye for little details make Midnight Sun a rare treat not only for comics fans, but a remarkable little piece of storytelling.Powered by Sidelines


text:
Resilient Regions is a neutral arena where the business sector, academia, municipalities and government agencies meet to solve regional challenges. Together we build a more resilient society with robust functions and flows – a society with the ability to quickly overcome and recover from societal pressures.
Resilient Communities
A Resilient Regions community addresses challenges in order to develop regional solutions. Members, the regional and local stakeholders with direct interests, responsibilities and needs, are the ones creating opportunities for strategic development initiatives, international knowledge exchange and access to "best practice".
Flows in focus
Different operations in a city are dependent on one another and bound together by flows. We offer cooperation and the development of functional cities with a focus on the flows of goods, services, money, people, energy and information. You can read more about us here.
Read more!


text: Hi Chuck Esterbrook, on 30-Nov-2001 you wrote: > On Thursday 29 November 2001 02:09 pm, Ian Bicking wrote: >> There's still something about Netscape 4.x, despite all it's lameness, >> that keeps me coming back from time to time. _It's just so much more >> responsive than all the modern browsers, even IE (though Opera could >> probably win me over if I gave it a chance). I can only recommend galeon 1.0 to all of you seeking for a better Linux browser. It combines the speed of NN4 with the superior rendering engine and privacy functions of Mozilla with added bonuses like "Auto Bookmarks" (bookmarking all open windows in case of a - rather rare - crash), the best bookmark editor/handling I have seen so far in ANY browser on x86 OSes, completely Mouse-configurable UI and arbitrary mixing of tabbed and windowed browsing. I guess once it gets to version 1.1, finishing off some more small bugs, I'll probably daresay its better than IE. Regards, Fionn


text:
81st Texas Legislature, Regular Session, House Bill 1841, Chapter 649
Description: Bill introduced by the Texas House of Representatives relating to he creation of the XS Ranch Municipal Utility District; providing authority to impose a tax and issue bonds; granting a limited power of eminent domain.
Date: June 19, 2009
Creator: Texas. Legislature. House of Representatives.
Item Type: Legislative Document
Partner: UNT Libraries Government Documents Department


text:
You get Free shipping on all orders shipped in the United States and US territories including Guam, US Virgin Islands and Puerto Rico.
You have a full 60 days to return your Audio Bible purchase. You have plenty of time to decide if your Audio Bible product is right for you. If you are not satisfied for any reason, just let us know. 1-800-758-0160 9AM to 9PM EST.
You pay NO restocking fee.
Benefits include:
We promise to never spam you, and just use your email address to identify you as a valid customer.
By Susanna H.
Came to India quickly and was easy to navigate. The blind lady really appreciated it!
Audio Bible
PO Box 6252
North Augusta, SC 29861
9am to 9pm EST


text:
The bug that prevented generall settings from working has been resolved.
Do as I say. Not as I do.
Forum Rules
i have 7 goyder river rainbows for sale at 5
dollars each. they range from 3-4 inches in
length. the ...
This ad is for TWO Dwarf Flag cichlid, 2-2.5", $
Eats frz brine and blood worm. Thanks for l ...


text:
SeamLink Zoot's proprietary stitch maximizes stretch, provides next-to-skin comfort, and minimizes chafing. UPF 50+ Protect yourself from harmful UV rays with UPF 50+ sun protection.
Thank you for visiting Zoot Womens Performance Tri Top - Exclusive To Wiggle Tri Tops on oakleysbikes.co.uk
We bring the products from many websites to one website for you to search, save and compare.


text:
Photo Gallery: A look back on the Norfolk Food and Drink Festival
15:28 08 October 2012
Archant © 2012
From the Battle of the Bangers to the Tour of Britain, have a look back at the events that have made this years festival one of the best yet.
There have been many great events in the Norfolk Food and Drink Festival that have showcased the best local produce that Norfolk has to offer.
To scroll through the pictures, see the photo gallery at the top-right of this page.


text:
Armchair Bow Tie Blue
Make little kids feel big with these fun, funky, Australian designed bean bag chairs that come wiRead More
Make little kids feel big with these fun, funky, Australian designed bean bag chairs that come with an inner calico bag for safety and ease of cleaning. The outer sleeve has a child proof zip and has been safety tested to Australian and New Zealand standards. Available in cute polka dots.
|100% Cotton
|Product Care
|Warm machine wash with like colours on a gentle cycle. Do not bleach soak or rub.
Standard Delivery
For smaller or lightweight items, Indigo Living will send your order by courier. After your order is dispatched from our warehouse, our customer service representative will send you an email with a link so that you can track your order. . There are different courier charges for different areas. Please see 'Accessories & Small items' under Delivery & Shipping to review the varying charges forHK, Kowloon, New Territories, Ma Wan and Discovery Bay. Indigo offers free delivery to HK, Kowloon and New Territories for purchases over HK$1000.
Hong Kong delivery within 3 working days
Express Delivery
Indigo Living offers Express delivery on accessories and small items. If you place an order before noon, you will receive your order on the same day. If you place the order after 12pm, then you will receive your order the following day. There is an additional charge of HK$200 for our Express delivery service.
Order by 2pm for next day delivery
Macau Delivery Info
Click & Collect
Click & Collect is a free online delivery option where you can easily buy online and collect accessories, small and some fragile items in-store. Our Flagship store in Horizon Plaza,our Landmark Prince's Building store as well as our Shatin and Cyberport stores currently accept Click & Collect orders. Please visit our store page to find your the store closest to you or see 'Click & Collect' for each store's collection schedule.
- Miller Junior Single BedHK$5,990.00
- Modern MooseHK$519.00
- Miller Junior 1 White Drawer Bedside TableHK$2,990.00
- SnurkHK$1,180.00