text:
RhoB modifies estrogen responses in breast cancer cells by influencing expression of the estrogen receptor
DOI: 10.1186/bcr3377
© Médale-Giamarchi et al.; licensee BioMed Central Ltd. 2013
Received: 6 August 2012
Accepted: 10 January 2013
Published: 22 January 2013
RhoB has been reported to exert positive and negative effects on cancer pathophysiology but an understanding of its role in breast cancer remains incomplete. Analysis of data from the Oncomine database showed a positive correlation between RhoB expression and positivity for both estrogen receptor alpha (ERα) and progesterone receptor (PR).
This finding was validated by our analysis of a tissue microarray constructed from a cohort of 113 patients and then investigated in human cell models.
We found that RhoB expression in tissue was strongly correlated with ERα and PR expression and inversely correlated with tumor grade, tumor size and count of mitosis. In human breast cancer cell lines, RhoB attenuation was associated with reduced expression of both ERα and PR, whereas elevation of RhoB was found to be associated with ERα overexpression. Mechanistic investigations suggested that RhoB modulates ERα expression, controlling both its protein and mRNA levels, and that RhoB modulates PR expression by accentuating the recruitment of ERα and other major co-regulators to the promoter of PR gene. A major consequence of RhoB modulation was that RhoB differentially regulated the proliferation of breast cancer cell lines. Interestingly, we documented crosstalk between RhoB and ERα, with estrogen treatment leading to RhoB activation.
Taken together, our findings offer evidence that in human breast cancer RhoB acts as a positive function to promote expression of ERα and PR in a manner correlated with cell proliferation.
Hormone therapy is recommended in breast cancers that express estrogen receptor alpha (ERα) and/or progesterone receptor (PR). This therapy is largely effective but there are nevertheless many cases of systemic resistance. A number of studies have addressed the question of the mechanisms of resistance to hormone therapy [1, 2]. ERα transcriptional effects are not only determined by ligands as estradiol (E2), but also by crosstalk between ERα and growth factor signaling [3]. The hierarchy among these associations is not known and various growth factor receptors are likely to be required [3].
Prenylated proteins such as Rho GTPases are key elements in growth factor signal transduction pathways [4]. A variety of growth factors present in the tumor microenvironment activate Rho proteins [5], especially RhoB [6–8]. As a Rho protein, RhoB cycles between GTP and GDP bound states, forming interactions with a variety of effectors that modulate activity and influence important processes in cancer [9]. RhoB, in contrast to its relatives RhoA and RhoC, has been shown to function as a tumor suppressor gene on the basis of investigations of genetically RhoB-deficient strains [10] and in human cancer cells [11–13]. RhoB is an immediate early response gene that is induced by a variety of stimuli, including growth factors [6, 14–21]. Although no mutation of Rho GTPases have been detected in human tumors, a correlation has been demonstrated between Rho protein overexpression and poor clinical outcome in breast cancers [22]. RhoB overexpression has been correlated to disease progression [23], although this is a controversial issue [24], and overexpression of guanine exchange factors (GEF) for Rho GTPases have been correlated to prognosis in breast cancers [25]. Indeed, breast tumor progression is accompanied by a decrease in expression of the pro-oncogenic RhoGEF Tiam1 [26]. Moreover, expression of Rho-GDI α, a negative regulator of Rho proteins, is reported to correlate with the outcome of patients with breast cancer treated by adjuvant chemotherapy [27]. From a molecular point of view, scaffold proteins involved in Rho functions, such as Rho-GDI or Dblx, have been observed as part of ERα-containing complexes [28, 29] with direct interaction between ERα and Rho-GDI [30].
Besides the suggested specific involvement of RhoB in ER signaling, there have been no detailed investigations in breast cancer cells, including the assessment of any correlation with the expression of hormone receptors in tumors. A major goal of the present study was therefore to determine the involvement of RhoB in hormone-dependent breast cancers and to investigate hypothesized crosstalks between RhoB and ERα signaling.
Materials and methods
Immunohistochemical analysis of tissue microarrays
A tissue microarray was constructed from the 113 patients described in Table S1 in Additional file 1. As detailed in Additional file 2, the clinical trial was conducted about 30 years ago with no consent required at that time. Cores (600 μm diameter) of histologically confirmed invasive breast carcinomas were extracted from the original paraffin blocks and re-embedded into a gridded recipient paraffin block using a tissue arrayer (Alphelys; Beecher Inc., Plaisir, France). For each case, three tumor cores and one normal breast core were taken from the original block.
Correlation of RhoB expression with clinical variables was assessed using Mann-Whitney and Spearman's rank tests. Univariate survival analysis was performed for disease-free survival by applying the log-rank test to RhoB expression levels stratified by median value. The Kaplan-Meier method was used to link the disease-free survival according to RhoB expression in the tumors.
Cell culture and reagents
The human breast adenocarcinoma cell lines MCF-7, ZR75, T47D, SK-BR-3 and MDA-MB-231 were obtained from the ATCC (Molsheim, France). Cells were grown routinely in DMEM (Lonza Levallois-Perret, France), supplemented with 5% fetal bovine serum (FBS; Pan-Biotech, Aidenbach, Germany). Hormonoresistant LCC2 cells (R Clarke, Karmanos Cancer Center, provided the parent MCF-7) were grown in F-12 (Lonza) phenol red-free medium, containing 5% dextran-coated charcoal (DCC)-treated FBS. Cryopreservation of cell cultures ranged from passage 1 to 10. Cells were used for experiments during up to 20 passages. Mouse embryonic fibroblasts (MEFs) were grown in DMEM with 10% FBS.
Where indicated, cells were deprived of E2 by growing them in phenol red-free medium containing 5% DCC-treated FBS. Cells were treated with 50 nM E2 or 50 ng/ml epidermal growth factor or 2 mM ICI-182, 780 (Sigma-Aldrich Chimie, Lyon, France).
siRNA transfection
Transient transfection of 40 nM siRNA was performed using Oligofectamine® (Life Technologies - Invitrogen, Saint Aubin, France) according to the manufacturer's instructions. The siRNA sequences of the oligonucleotide duplexes (Eurogentec, Angers, France) were siB1 (5'-CCGUCUUCGAGAACUAUGU-dTdT-3') and siB2 (5'-UGAUAUCCCUUGUCUGUAA-dTdT-3'), siER (5'-GGGAGAAUGUUGAAACACA-dTdT-3') and the nonspecific sequence siControl (5'-GACGUGGGACUGAAGGGGU-dTdT-3').
Adenoviral constructs expressing RhoB and transduction protocol
Replication-defective (ΔE1, E3) adenoviral vectors expressing RhoB under the transcriptional control of the CMV promoter were constructed with the Adeasy System (Qbiogen, Illkirch, France) as described previously [31]. Cells were transduced with adenoviral vectors (control empty vector or expressing RhoB) at a multiplicity of infection of 300:1.
Western blot analysis of human cell lines
Cells were lysed and protein analyzed by SDS-PAGE [18]. Antibodies were used against RhoB (sc-180), ERα (sc-543) and, ERβ (sc-53494) from Santa Cruz Biotechnology, phospho-ERα (Ser118, 2511 and Ser167, 2514) from Cell Signaling (Saint Quentin Yvelines, France), β-actin (MAB1501) from Chemicon (Merck Millipore; Darmstadt, Germany), and secondary antibodies were anti-mouse (MP21120) and anti-rabbit (MP23145) horseradish peroxidase from Interchim (Montluçon, France) using a chemiluminescence detection kit (ECL; Pierce, Thermo Fisher Scientific, Courtaboeuf, France). Protein abundance was quantified by Image Quant TL analysis (GE Healthcare, Velizy-Villacoublay, France).
Analysis of mouse embryonic fibroblasts generated from RhoB-deficient mice
Heterozygous (+/-) and homozygous (-/-) mutant strains of RhoB-deficient mice [10] were kindly provided by G Prendergast (Lankenau Institute for Medical Research, Wynnewood, PA, USA). Claudius Regaud Institute animal ethics committee approval was obtained (# ICR-2012-001-A) for the use of the animal model and the study protocols. Mice were housed in polycarbonate cages in controlled conditions. MEFs were generated [10] before lysis [18]. Protein were extracted and analyzed as described above with antibody against murine ERα (sc-542) or RhoB (sc-180; Santa Cruz Biotechnology).
GST pull-down assay
The level of activated RhoB and GTP-bound RhoB protein was measured using the GST fusion protein containing the Rho binding domain of Rhotekin [8]. The amount of GTP-bound RhoB and the total amount of RhoB in cell lysates were determined by western blot as described above [32].
The level of ERα and PR staining was determined by evaluation of the percentage of tumor-stained cells and staining intensity, allowing assessment of an IRS.
Quantitative reverse-transcribed PCR
Total RNA was isolated 16 hours after stimulation by E2, extracted using the RNeasy kit following the manufacturer's instructions (Qiagen, Courtaboeuf, France) and reverse-transcribed using the iScript™ cDNA Synthesis Kit (Biorad, Marnes la Coquette, France). Quantitative PCR was performed with an iQreal-time PCR detection system (Biorad). The specific human primer pairs used were: for GAPDH, 5'-TGCACCACCAACTGCTTAGC-3' and 5'-GGCATGGACTGTGGTCATGAG-3'; for 28s, 5'-TCGCTGGGTCTTGGATGTC-3' and 5'-AGCAGATTGTGACAGACCATTCC-3'; for PR, 5'-CGCGCTCTACCCTGCACTC-3' and 5'-TGAATCCGGCCTCAGGTAGTT-3'; and for ERα, 5'-CCACCAACCAGTGCACCATT-3' and 5'-GGT CTTTTCGTATCCCACCTTTC-3' (Eurogentec).
Luciferase assay
Development of stable transfectants of MCF-7 cells (MELN cells) has been described previously [34]. These cells, kindly provided by P Balaguer (INSERM 540, France), were established by transfecting MCF-7 cells with ERE-β-globin-luc-SV-Neo plasmid and thus express luciferase in an estrogen-dependent manner.
Three days after siRNA transfection, MELN cells were seeded in DMEM-DCC-treated FBS during 3 days. They were then treated with E2 or ethanol for 16 hours. Cells were then lysed in reporter lysis buffer (Promega, Charbonnières Les Bains, France). The luciferase activity was measured with luciferase assay reagent (Promega) according to the manufacturer's instructions. Protein concentration was measured to normalize the luciferase activity data.
Chromatin immunoprecipitation assay
Chromatin immunoprecipitation assays were performed with modifications of the procedure of Metivier and colleagues [35] as described elsewhere [36]. Briefly, 106 cells were synchronized by 3 days of culture in DMEM 5% DCC-treated FCS and treated during 1 hour with E2. Immunoprecipitation antibodies were ERα (sc-543), HDAC1 (sc-6299), and polII (sc-899) from Santa Cruz Biotechnology, and acetylated histone H3 (ab1791) and H4 (ab193) from Abcam (Paris, France). Quantitative PCR were performed on an iCycler (Biorad) using the primers 5'-GGCGACACAGCAGTGGGGAT-3' and 5'-TCTCCTCCCTCTGCCCCTATATTC-3' (Eurogentec) to amplify the fragment of the human PR promoter flanking the +745 AP-1 site [37].
Cell growth determination
Forty-eight hours after siRNA transfection or transduction, cells were seeded in DMEM 5% FBS with ethanol or E2 and counted daily during the next 4 days with a coulter counter (Beckman Coulter, Grenoble, France).
RhoB expression correlates positively with receptor status and negatively with grade in human breast tumors
Using the Oncomine microarray database, a correlation between RhoB and either ERα or PR expression was documented in datasets from 19 breast cancer studies. Moreover, four studies showed an inverse correlation between RhoB expression and tumor grade. To confirm the clinical relevance of RhoB expression levels in breast cancer oncogenesis and outcome, we compared the expression levels of RhoB in breast carcinomas from a cohort of 113 patients treated or not by tamoxifen in an adjuvant setting in a randomized prospective study. Patient characteristics are described in Table S1 in Additional file 1. ERα and PR assessments were initially performed with biochemistry techniques at the time of diagnosis (between 1980 and 1983). They have all been performed again at the time of analysis, in parallel with RhoB assessment. After new pathological analysis, 65 tumors were classified as grade I and II, 74 were ERα-positive, and 59 were PR-positive. Among these tumors, 23 presented a lymphovascular invasion and 39 cases presented with positive lymph nodes. After randomization, we determined that age, ERα and PR status, histological grade, and type and lymphovascular invasion status were similar in the two groups of patients, whether treated or not. Nonetheless, patients treated with tamoxifen (n = 62) had less favorable prognostic factors regarding positive lymph nodes, pathological tumor size and number of mitoses.
Figure 1A shows the RhoB immunohistochemistry stainings of two representative tumors of patients displaying opposite hormone receptor status (ERα/PR-negative and ERα/PR-positive), illustrating the direct correlation between RhoB and both ERα and PR expression. Enlargement of the photographs (Figure 1B) illustrates in the tumor cells of the ERα/PR-negative patient that RhoB is present and specifically cytoplasmic, although the staining is weak. For the ERα/PR-positive patient, in addition to cytoplasmic staining, a strong staining was also observed at nucleus level.
Statistical analysis of the results indicated that RhoB expression in tumors is strongly correlated with the percentage of ERα (Spearman's ρ = 0.3659, P = 0.001) and PR (ρ = 0.2544, P = 0.0076) expression, but inversely correlated with histological tumor size (ρ = -0.2344, P = 0.0166) and number of mitoses (ρ = -0.2009, P = 0.0362). We divided the patients with ERα-positive tumors into two groups with either low or high RhoB expression, and in each group we further divided the patients into groups that did or did not receive tamoxifen. This analysis argued that the level of RhoB expression was not correlated with disease-free survival of patients with ERα-positive tumors, regardless of tamoxifen treatment or not (Kaplan-Meier curve shown in Figure S1 in Additional file 3).
The RhoB IRS (see Materials and methods) integrating both the percentage and intensity of the staining (Figure 1C) was significantly higher in ERα-positive tumors (median 10.5 (3 to 12)) as compared with ERα-negative tumors (median 8 (1 to 12)), in PR-positive tumors (median 9 (3 to 12)) as compared with PR-negative tumors (median 8 (1 to 12)), and in patients with tumor grade I and II (median 9 (3 to 12)) as compared with grade III (median 8 (1 to 12)). The line in the center of each box represents the median value of the distribution, and the upper and lower ends of the box are the upper and lower quartiles, respectively. The RhoB level of expression was also higher in the smaller tumor size (≤ 2 cm, median 12 (2 to 12)) as compared with larger tumors (> 2 cm, median 8 (1 to 12)). The RhoB IRS score was not correlated with the presence of lymphovascular invasion (P = 0.26), nor with the presence of lymph node invasion (P = 0.74).
RhoB regulates ERα expression in MCF-7, in vivo and in other breast cancer cell lines
To rule out the possibility of off-target effects of the siRNA approach, we transduced MCF-7 cells with an adenoviral vector expressing RhoB (Figure 2A, right panel). The observed 4.5-fold overexpression of RhoB increased the expression of ERα (186%), further supporting the hypothesized regulatory relationship.
We extended the study of RhoB downregulation on ERα expression to additional cell lines (Figure S2 in Additional file 4), confirming a decrease of ERα expression with RhoB depletion using siB1 and siB2 in T47D and ZR75 cells (hormone-dependent cells) or in LCC2 cells (hormone-resistant cells). We confirmed the involvement of RhoB on ERα expression in vivo using mice that are genetically deficient in RhoB (Figure 2B). A major decrease of ERα expression was visualized in MEFs collected from RhoB+/- mice with an even more dramatic decrease in MEFs collected from RhoB-/- mice. These results extended the support for a hypothesized regulatory relationship between RhoB and ERα.
We also studied the levels of ERα phosphorylated forms whose involvement as prognostic markers is discussed below (Figure 2C). We observed that the decrease of ERα expression observed when RhoB expression is downregulated is not associated with any significant specific change of P-Ser118 or P-Ser167 ERα level. Indeed, the ratio phosphorylated/total ERα is not modified after RhoB inhibition.
We then demonstrated that ERα expression is dramatically decreased in the presence of the pure anti-estrogen ICI-182, 780, even when RhoB is overexpressed (Figure 2D).
Estrogen induces RhoB activation
Together these results suggested that RhoB and estrogen signaling are integrated into a feed-forward loop that may positively modify the biological effects of estrogen treatment.
RhoB regulates ERα, but not ERβ, expression in the absence and presence of estradiol in MCF-7 cells
We analyzed further the effect of RhoB downregulation on ERα and ERβ expression in the absence and presence of E2 (Figure 3B). As expected, E2 treatment induced a major significant decrease of ERα expression and concomitantly of RhoB expression. In the presence of siB2, a 36 to 41% decrease of ERα expression was observed in the absence of E2 and 48 to 52% in the presence of E2. In contrast, ERβ expression was not clearly altered by RhoB downregulation (Figure 3B). Using immunocytochemistry, we confirmed these results suggesting that RhoB regulates ERα expression and observed no dramatic changes of RhoB subcellular localization within MCF-7 cells (Figure 3C). ERα was highly concentrated in the nucleus of the untreated control with a significant staining of the related cytoplasm. In the presence of E2, the staining intensities of both nuclei and cytoplasms were clearly decreased with no more detectable staining in the cytoplasm, confirmed by IRS assessment. For siB2-treated cells, a similar major decrease of the labeling intensity was observed in the cytoplasm and the nucleus, both in the presence and the absence of E2. In the presence of E2, the IRS score was maintained at a value of 2 because, in spite of the difference from 30 to 20% of stained cells induced by siB2 treatment, the intensity is kept very low in both cases (value of 1).
We then investigated whether RhoB downregulation modulates ERα mRNA expression (Figure 3D). We first confirmed the expected decrease of ERα mRNA expression in control cells treated by E2 alone (to 0.49). In the cells treated with siB2, a dramatic decrease of ERα mRNA was observed in the absence of E2 (from 1 to 0.53) and much lighter in the presence of E2 (from 0.49 to 0.37).
RhoB promotes ERα transcriptional activation function induced by estrogen
Regarding PR, a major target gene of ERα, PR mRNA expression was induced in the presence of E2 (7.7-fold) in the siRNA control cells (Figure 4B). In cells treated with siB1, there was a significant decrease of PR mRNA levels as compared with their respective control, both without and with E2 (rates 0.5 and 0.6, respectively). Consequently, E2 induction was maintained for cells transfected by both siRNA control and siB1 (respectively 7.7-fold and ninefold).
Using immunocytochemistry, we confirmed that RhoB regulates ERα (Figure 3C) plus PR (Figure 4C) expression, with no dramatic changes of their subcellular localization within MCF-7 cells. PR is mainly detected in the nuclei of the untreated control. Besides, the presence of siB2 again significantly decreases IRS scores both in the absence and presence of E2.
Cells treated with siB2 exhibit low levels of ERα protein and mRNA in both the absence and presence of E2 (Figure 3B, C, D). Nonetheless, the effects of RhoB on PR expression may be supported in part by other mechanisms. We used chromatin immunoprecipitation analysis to study the effect of RhoB inhibition on the recruitment of ERα, ER transcriptional co-factors and RNA polymerase II onto PR gene promoter 1 hour after E2 stimulation (Figure 4D). Following siB1 transfection, the recruitment of RNA polymerase II to the PR gene promoter dramatically decreased (rate 0.3 compared with siControl). Recruitment of acetylated H3 and H4 histones were also clearly decreased by RhoB downregulation (rates 0.36 and 0.33, respectively). Moreover, RhoB downregulation induced a significant decrease in the recruitment of ER to the PR gene promoter (rate 0.52) paralleled by an increased in recruitment of the ERα co-repressor HDAC1 (rate 1.67). Together, these results indicate that RhoB may support to some extent ERα transcriptional activation function by interfering with its cofactor recruitment, besides the direct effect on ERα expression itself.
RhoB induces proliferation in ER-positive but not in ER-negative breast cancer cell lines
In conclusion, we documented that RhoB had stimulatory effects on proliferation via ERα signaling that paralleled its effects on hormone receptor expression.
Although the tumor suppressor function of RhoB has been documented in many human cancers [11, 12], RhoB overexpression was suggested to be associated with tumor progression in breast cancers. Using cellular and human breast tumor analytical approaches, we have shown a positive crosstalk between RhoB and ERα expression and the critical role of RhoB in regulation of the proliferation of ERα-expressing breast cancer cells.
Our data bring together three lines of evidence to support the crosstalk between ERα and RhoB. First, in human breast cancer tissues we clearly showed a strong correlation between RhoB expression and the expression of ERα and PR. Moreover RhoB expression was associated with a low tumor grade and size, suggesting that RhoB expression is correlated with good prognosis markers. The second strand of evidence came from cellular results showing that the level of RhoB controls the expression of ERα in ERα-positive breast cancer cell in the presence or absence of E2. The positive modulation of ERα expression by RhoB was evidenced both at the protein and mRNA levels. We showed that the ERα mRNA level is controlled by RhoB, suggesting that transcriptional regulation could play a key role in this regulation. The phosphorylation of Ser167 of ERα in tumors has been related to longer overall survival whereas the phosphorylation of Ser118 could be a good prognostic marker [38, 39]. We found a dramatically decreased level of ERα phosphorylation at both serine sites that can be attributed to the major decrease in total ERα expression (RhoB downregulation induced no significant change in the phosphorylated/total ERα ratio). Third, we clearly demonstrated in vivo the RhoB control of ERα expression in MEF cells derived from a RhoB-/- mouse model. This regulation is of interest in the context of the epithelial-stromal interactions, particularly given that breast adipose fibroblasts determine the expression of aromatase [40]. Further, we demonstrated that ERα controls the activation and expression of RhoB. Notably, E2 treatment induces an increase of active GTP-RhoB within 30 minutes, without changing RhoB expression at that time.
ERα upregulates or downregulates the transcription of hundreds of genes [41] and PR is a well-known ERα target gene with a major physiological role in cell proliferation. Both PR mRNA and protein expression were significantly decreased as a consequence of RhoB and related ERα downregulations. Further findings evidenced a clear decrease of the recruitment of RNA polymerase II, acetylated H3/H4 histones and ERα onto the promoter of the PR gene. In parallel, we described the recruitment of the major transcriptional co-repressor HDAC1, which is known to repress RhoB expression [42]. These results provide the demonstration of a regulatory role for RhoB in ERα expression and in the balance of the associated co-regulators of ERα to control transcription of its target genes. The possibility of a direct interaction of RhoB with the ERα-dependent transcriptional machinery should not be excluded since a direct interaction between ERα and RhoGDIα in breast cancer cells has been demonstrated [30] and we have shown here a strong nuclear localization of RhoB in ERα-positive tumor tissues.
In contrast to other cancer models, RhoB is critical for the proliferation of ERα-expressing breast cancer cells, suggesting its role as a positive regulator in this model. It is noteworthy that the RhoB effect is also observed on the proliferation of the ERα-positive, tamoxifen-resistant LCC2 model cell line. Inversely, in ERα-negative cell lines (SK-BR-3 and MDA-MB-231), RhoB has no effect on proliferation - thus reinforcing the idea that RhoB promotes cell proliferation through ERα expression. These results suggest that RhoB downregulation in breast cancer cells could be associated with tumor progression in parallel to ERα extinction, with a chronology that remains to be elucidated.
We have demonstrated that RhoB GTPase is a key inducer of ERα and a key regulator of PR expression. RhoB acts through various complex mechanisms underlying a feed-forward loop that may control estrogen effects, including cell proliferation. Our new findings shed light on the role of RhoB in tumorigenesis involving a dual effect conferred by cellular context with a potential pro-oncogenic function in hormone-dependent breast cancer cells.
dextran-coated charcoal
Dulbecco's modified Eagle's medium
estrogen receptor
fetal bovine serum
ImmunoReactive Score
mouse embryonic fibroblast
polymerase chain reaction
progesterone receptor
reverse transcriptase
- siRNA:
small interfering RNA.
This work was supported by the National Institute of Cancer, Canceropole Grand Sud-Ouest, Fondation RITC (C Silvagni), INSERM, Claudius Regaud Institute, University of Toulouse (UPS) and the French Ministry of Research (MRES). Thanks are due to GC Prendergast for fruitful discussion and reviewing the manuscript.
Authors' Affiliations
- Musgrove EA, Sutherland RL: Biological determinants of endocrine resistance in breast cancer. Nat Rev Cancer. 2009, 9: 631-643. 10.1038/nrc2713.View ArticlePubMedGoogle Scholar
- Miller TW, Balko JM, Arteaga CL: Phosphatidylinositol 3-kinase and antiestrogen resistance in breast cancer. J Clin Oncol. 2011, 29: 4452-4461. 10.1200/JCO.2010.34.4879.View ArticlePubMedPubMed CentralGoogle Scholar
- Fox EM, Andrade J, Shupnik MA: Novel actions of estrogen to promote proliferation: integration of cytoplasmic and nuclear pathways. Steroids. 2009, 74: 622-627. 10.1016/j.steroids.2008.10.014.View ArticlePubMedGoogle Scholar
- Guilluy C, Garcia-Mata R, Burridge K: Rho protein crosstalk: another social network?. Trends Cell Biol. 2011, 21: 718-726. 10.1016/j.tcb.2011.08.002.View ArticlePubMedPubMed CentralGoogle Scholar
- Jaffe AB, Hall A: Rho GTPases in transformation and metastasis. Adv Cancer Res. 2002, 84: 57-80.View ArticlePubMedGoogle Scholar
- Jahner D, Hunter T: The ras-related gene rhoB is an immediate-early gene inducible by v-Fps, epidermal growth factor, and platelet-derived growth factor in rat fibroblasts. Mol Cell Biol. 1991, 11: 3682-3690.View ArticlePubMedPubMed CentralGoogle Scholar
- de Cremoux P, Gauville C, Closson V, Linares G, Calvo F, Tavitian A, Olofsson B: EGF modulation of the ras-related rhoB gene expression in human breast-cancer cell lines. Int J Cancer. 1994, 59: 408-415. 10.1002/ijc.2910590320.View ArticlePubMedGoogle Scholar
- Gampel A, Mellor H: Small interfering RNAs as a tool to assign Rho GTPase exchange-factor function in vivo. Biochem J. 2002, 366: 393-398. 10.1042/BJ20020844.View ArticlePubMedPubMed CentralGoogle Scholar
- Karlsson R, Pedersen ED, Wang Z, Brakebusch C: Rho GTPase function in tumorigenesis. Biochim Biophys Acta. 2009, 1796: 91-98.PubMedGoogle Scholar
- Liu AX, Rane N, Liu JP, Prendergast GC: RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells. Mol Cell Biol. 2001, 21: 6906-6912. 10.1128/MCB.21.20.6906-6912.2001.View ArticlePubMedPubMed CentralGoogle Scholar
- Adnane J, Muro-Cacho C, Mathews L, Sebti SM, Munoz-Antonia T: Suppression of rho B expression in invasive carcinoma from head and neck cancer patients. Clin Cancer Res. 2002, 8: 2225-2232.PubMedGoogle Scholar
- Mazieres J, Antonia T, Daste G, Muro-Cacho C, Berchery D, Tillement V, Pradines A, Sebti S, Favre G: Loss of RhoB expression in human lung cancer progression. Clin Cancer Res. 2004, 10: 2742-2750. 10.1158/1078-0432.CCR-03-0149.View ArticlePubMedGoogle Scholar
- Zhou J, Zhu Y, Zhang G, Liu N, Sun L, Liu M, Qiu M, Luo D, Tang Q, Liao Z, Zheng Y, Bi F: A distinct role of RhoB in gastric cancer suppression. Int J Cancer. 2011, 128: 1057-1068. 10.1002/ijc.25445.View ArticlePubMedGoogle Scholar
- Lebowitz PF, Davide JP, Prendergast GC: Evidence that farnesyltransferase inhibitors suppress Ras transformation by interfering with Rho activity. Mol Cell Biol. 1995, 15: 6613-6622.View ArticlePubMedPubMed CentralGoogle Scholar
- Zalcman G, Closson V, Linares-Cruz G, Lerebours F, Honore N, Tavitian A, Olofsson B: Regulation of Ras-related RhoB protein expression during the cell cycle. Oncogene. 1995, 10: 1935-1945.PubMedGoogle Scholar
- Gampel A, Parker PJ, Mellor H: Regulation of epidermal growth factor receptor traffic by the small GTPase rhoB. Curr Biol. 1999, 9: 955-958. 10.1016/S0960-9822(99)80422-9.View ArticlePubMedGoogle Scholar
- Engel ME, Datta PK, Moses HL: RhoB is stabilized by transforming growth factor beta and antagonizes transcriptional activation. J Biol Chem. 1998, 273: 9921-9926. 10.1074/jbc.273.16.9921.View ArticlePubMedGoogle Scholar
- Skuli N, Monferran S, Delmas C, Lajoie-Mazenc I, Favre G, Toulas C, Cohen-Jonathan-Moyal E: Activation of RhoB by hypoxia controls hypoxia-inducible factor-1α stabilization through glycogen synthase kinase-3 in U87 glioblastoma cells. Cancer Res. 2006, 66: 482-489. 10.1158/0008-5472.CAN-05-2299.View ArticlePubMedGoogle Scholar
- Fritz G, Kaina B, Aktories K: The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments. J Biol Chem. 1995, 270: 25172-25177. 10.1074/jbc.270.42.25172.View ArticlePubMedGoogle Scholar
- Canguilhem B, Pradines A, Baudouin C, Boby C, Lajoie-Mazenc I, Charveron M, Favre G: RhoB protects human keratinocytes from UVB-induced apoptosis through epidermal growth factor receptor signaling. J Biol Chem. 2005, 280: 43257-43263. 10.1074/jbc.M508650200.View ArticlePubMedGoogle Scholar
- Srougi MC, Burridge K: The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. PLoS One. 2011, 6: e17108-10.1371/journal.pone.0017108.View ArticlePubMedPubMed CentralGoogle Scholar
- Tang Y, Olufemi L, Wang MT, Nie D: Role of Rho GTPases in breast cancer. Front Biosci. 2008, 13: 759-776. 10.2741/2718.View ArticlePubMedGoogle Scholar
- Fritz G, Brachetti C, Bahlmann F, Schmidt M, Kaina B: Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters. Br J Cancer. 2002, 87: 635-644. 10.1038/sj.bjc.6600510.View ArticlePubMedPubMed CentralGoogle Scholar
- Jiang WG, Watkins G, Lane J, Cunnick GH, Douglas-Jones A, Mokbel K, Mansel RE: Prognostic value of rho GTPases and rho guanine nucleotide dissociation inhibitors in human breast cancers. Clin Cancer Res. 2003, 9: 6432-6440.PubMedGoogle Scholar
- Lane J, Martin TA, Mansel RE, Jiang WG: The expression and prognostic value of the guanine nucleotide exchange factors (GEFs) Trio, Vav1 and TIAM-1 in human breast cancer. Int Semin Surg Oncol. 2008, 5: 23-10.1186/1477-7800-5-23.View ArticlePubMedPubMed CentralGoogle Scholar
- Stebel A, Brachetti C, Kunkel M, Schmidt M, Fritz G: Progression of breast tumors is accompanied by a decrease in expression of the Rho guanine exchange factor Tiam1. Oncol Rep. 2009, 21: 217-222.PubMedGoogle Scholar
- Ronneburg H, Span PN, Kantelhardt E, Dittmer A, Schunke D, Holzhausen HJ, Sweep FC, Dittmer J: Rho GDP dissociation inhibitor alpha expression correlates with the outcome of CMF treatment in invasive ductal breast cancer. Int J Oncol. 36: 379-386.
- Rubino D, Driggers P, Arbit D, Kemp L, Miller B, Coso O, Pagliai K, Gray K, Gutkind S, Segars J: Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action. Oncogene. 1998, 16: 2513-2526. 10.1038/sj.onc.1201783.View ArticlePubMedGoogle Scholar
- Su LF, Knoblauch R, Garabedian MJ: Rho GTPases as modulators of the estrogen receptor transcriptional response. J Biol Chem. 2001, 276: 3231-3237. 10.1074/jbc.M005547200.View ArticlePubMedGoogle Scholar
- El Marzouk S, Schultz-Norton JR, Likhite VS, McLeod IX, Yates JR, Nardulli AM: Rho GDP dissociation inhibitor alpha interacts with estrogen receptor alpha and influences estrogen responsiveness. J Mol Endocrinol. 2007, 39: 249-259. 10.1677/JME-07-0055.View ArticlePubMedGoogle Scholar
- Couderc B, Pradines A, Rafii A, Golzio M, Deviers A, Allal C, Berg D, Penary M, Teissie J, Favre G: In vivo restoration of RhoB expression leads to ovarian tumor regression. Cancer Gene Ther. 2008, 15: 456-464. 10.1038/cgt.2008.12.View ArticlePubMedGoogle Scholar
- Lajoie-Mazenc I, Tovar D, Penary M, Lortal B, Allart S, Favard C, Brihoum M, Pradines A, Favre G: MAP1A light chain-2 interacts with GTP-RhoB to control epidermal growth factor (EGF)-dependent EGF receptor signaling. J Biol Chem. 2008, 283: 4155-4164.View ArticlePubMedGoogle Scholar
- Delfour C, Roger P, Bret C, Berthe ML, Rochaix P, Kalfa N, Raynaud P, Bibeau F, Maudelonde T, Boulle N: RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. J Mol Diagn. 2006, 8: 157-169. 10.2353/jmoldx.2006.050105.View ArticlePubMedPubMed CentralGoogle Scholar
- Balaguer P, Francois F, Comunale F, Fenet H, Boussioux AM, Pons M, Nicolas JC, Casellas C: Reporter cell lines to study the estrogenic effects of xenoestrogens. Sci Total Environ. 1999, 233: 47-56. 10.1016/S0048-9697(99)00178-3.View ArticlePubMedGoogle Scholar
- Metivier R, Penot G, Hubner MR, Reid G, Brand H, Kos M, Gannon F: Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter. Cell. 2003, 115: 751-763. 10.1016/S0092-8674(03)00934-6.View ArticlePubMedGoogle Scholar
- Baron S, Escande A, Alberola G, Bystricky K, Balaguer P, Richard-Foy H: Estrogen receptor alpha and the activating protein-1 complex cooperate during insulin-like growth factor-I-induced transcriptional activation of the pS2/TFF1 gene. J Biol Chem. 2007, 282: 11732-11741.View ArticlePubMedGoogle Scholar
- Petz LN, Ziegler YS, Schultz JR, Kim H, Kemper JK, Nardulli AM: Differential regulation of the human progesterone receptor gene through an estrogen response element half site and Sp1 sites. J Steroid Biochem Mol Biol. 2004, 88: 113-122. 10.1016/j.jsbmb.2003.11.008.View ArticlePubMedGoogle Scholar
- Jiang J, Sarwar N, Peston D, Kulinskaya E, Shousha S, Coombes RC, Ali S: Phosphorylation of estrogen receptor-alpha at Ser167 is indicative of longer disease-free and overall survival in breast cancer patients. Clin Cancer Res. 2007, 13: 5769-5776. 10.1158/1078-0432.CCR-07-0822.View ArticlePubMedGoogle Scholar
- Murphy LC, Niu Y, Snell L, Watson P: Phospho-serine-118 estrogen receptor-alpha expression is associated with better disease outcome in women treated with tamoxifen. Clin Cancer Res. 2004, 10: 5902-5906. 10.1158/1078-0432.CCR-04-0191.View ArticlePubMedGoogle Scholar
- Bulun SE, Lin Z, Zhao H, Lu M, Amin S, Reierstad S, Chen D: Regulation of aromatase expression in breast cancer tissue. Ann N Y Acad Sci. 2009, 1155: 121-131. 10.1111/j.1749-6632.2009.03705.x.View ArticlePubMedGoogle Scholar
- Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M: Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006, 38: 1289-1297. 10.1038/ng1901.View ArticlePubMedGoogle Scholar
- Wang S, Yan-Neale Y, Fischer D, Zeremski M, Cai R, Zhu J, Asselbergs F, Hampton G, Cohen D: Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line. Oncogene. 2003, 22: 6204-6213. 10.1038/sj.onc.1206653.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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|Expansive Natal Charts
|Compatibility Reports
|Mini - Chart Configurations
and Graphics
|Mini Insight Report
|15, 30, 60, 90 Minute Phone Readings
|6 or 12 Month Transits & Progressions Reports
|Unknown Birth Time
|e-Book - Using Your North & South Node
|Aspects & Their Symbols
|Applying & Separating Aspects
|Astrology Brain Dump™
|Dignity & Debility
|Glossary of Terms
|Grand Cross
|Grand Trine
|Moon in a Sign
|Moon Phases
|North & South Nodes
|Pet Sun Signs
|Planetary Glyphs
|Retrograde Motion
|Rising Signs
|Rising Sign Calculator
|Sun Signs
|Transits and Progressions
|2017 Void of Course Moon Calendar
|2017 Major Aspects & Astro-Memes
|2016 Retrograde Planetary Calendar
|2016 Moon Phase Calendar
|North & South Node Calendar
|Saturn in Sagittarius Transiting Degrees Calendar
|North Node in Virgo
South Node in Pisces Transiting Degrees Calendar
|Mars Moving Through The Signs Calendar
|Jupiter Moving Through The Signs Calendar
|Saturn Moving Through The Signs Calendar
|Neptune Moving Through The Signs Calendar
|Uranus Moving Through The Signs Calendar
|Pluto Moving Through The Signs Calendar
|Saturn in Virgo through the Houses
|North Node Virgo & South Node in Pisces
|Scorpio Moon Shopping & Surgery
|What is a Void of Course Moon
|How are horoscopes written?
|How can I be a Christian & practice Astrology?
|What is a Natal Chart?
If you are looking at a Natal Chart that I wrote for you, the YOD in the middle of your Chart Wheel will be the color BLUE for the sextile lines and the color RED for the quincunx lines. The picture to the right shows the quincunx lines in green. In a chart that I write, you will see these quincunx lines in RED.
A "Yod" is a triangular pattern in an astrology chart, which consists of 3 connecting points. It is an isosceles triangle with two long equal sides and a short side. The triangle has two 150 degree angles (quincunx) and one 60 degree angle (sextile). The triangle is pointing directly at something, which astrologers refer to as "The Finger of God," because it is a direct indicator giving the chart-holder a clue. The "yod" or "yud" is named for the 10th letter of the Hebrew alphabet which means, "Finger of Yahweh."
View the Yod in: Martha Stewart's Chart below. It's pointing directly at her Moon in Sagittarius in her 2nd house. It's saying, "USE YOUR MOON IN SAGITTARIUS IN YOUR 2ND HOUSE OF MONEY!" Sagittarius is "global and foreign affairs" and Martha certainly is global. Ten bucks says she will be bigger and better than ever and even more global.
The "Yod" will give you direct clues to opportunities for certain action, although the opportunities can be unconsciously restricted since you are continually making adjustments in your Life. So the bottom line here is; if you know you have a "yod" in your personal astrology chart, then it is telling you to MAKE UP YOUR MIND . . . MAKE A CHOICE . . . THERE'S AN ARROW POINTING AT THE ANSWER . . . CHOOSE TO DO WHAT THE ARROW IS POINTING TO!!!
The planets involved in the "yod" are usually not compatible with each other, so the energy isn't necessarily the easiest. But again, if you look at where the "yod" is pointing, it's a direct hit. Yod's have an element of destiny in their pattern. This pattern is usually bringing forward something that is needed in order to make further progress. Sometimes progress doesn't feel that great going through it, but once the individual has moved through the energy, there is gratitude for the lesson.
For example: IF there is a "Yod" that is pointing directly at Neptune in Aquarius then Neptune is the planet of illusions, dreams, sleep, anesthesia, drugs, feeling without physical contact and psychics. It is also cold because it is so distant from the Sun. It is moist, neutral and convertible. It is also mysterious and questionable. So now what do you do with this information?
Keeping in mind that Uranus is currently retrograde in Aquarius and with the constant doubt and indecision, it would be a time to take the bull by the horns. Aquarian's motto is "I know, therefore I AM," and in order to move forward they have to bust through the illusion of doubt. Doubt is the opposite of what they "know." Uranus represents sudden changes and that would mean it would be a Good time to go with flow of change and learn from the change, keeping in mind not to be fooled by illusions.
Yod's happen all the time and sometimes there may be as many as 6 or more Yod's in one day, pointing at various planets in a particular sign.
If you have an astrological finger pointing right at you, it is begging you to look at it and find the clue. If a "Yod" appears in your natal astrology chart and depending where the "yod" is, this will determine if this lesson is about relationships, career, family, money, children, etc.
**This web site's goal is to provide you with information that may be useful in attaining optimal health. Nothing in it is meant as a prescription or as medical advice. You should check with your physician before implementing any changes in your exercise or lifestyle habits, especially if you have physical problems or are taking medications of any kind.

subset: commoncrawl


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Wholesale Golf Equipment
Both R1 fairway wood and driver apparently will feature an all-lofts-in-one-head design similar to R11 and R11s driver. TaylorMade may even use a refined head shape with weights in the R1 placed closer to the face.
Print this page
PING engineers made the iron club face larger and added 27 grams to the perimeter for a higher MOI. The
TaylorMade R9 Irons(US Model) is on sale at Golf Wholesale >> Velocity-Control Chamber in 4 - 6-irons for increased consistency,
The TaylorMade Tour Preferred CB Iron Set is the latest and greatest from one of the top names in golf.
The Ping K15 Driver has an elongated head design that combines with an external weight pad to position the center
The Callaway RAZR Hawk Driver is uncompromised performance. Once again Callaway has made a giant leap forward with Forged Composite
MB irons feature a uniform muscle shape that provides more mass behind the impact area to achieve the quintessential soft,
The R11 Rescue hybrid offers high performance with bold aesthetics. Its distinctive white crown and black face makes the Rescue
Callaway Golf core technologies like VFT, S2H2, a 360-Degree Undercut Channel and Modified Tru-Bore cooperate with each other. This perfects
The MP-53's distinctive Diamond Muscle ensures that greater forgiveness is not achieved at the cost of workability or feel. Created
The forged, multi-metal design, of the Anser Forged Irons combines an 8620 steel body, a dense tungsten sole, and machined
The white 460cc head features a 3° Flight Control Technology sleeve, a new 5-way Adjustable Sole Plate and Taylormade's Movable
Callaway has reached a new level of performance in a forged iron, offering avid players the chance to play with
The 445cc Titleist 910D3 driver delivers longer distance through a technologically advanced head design with a responsive, fast face insert.
The Mizuno MP-63 Iron Set is the evolution of Mizuno's most popular Tour iron. The legendary Grain Flow Forging process
The G15 driver is engineered for golfers seeking a maximum forgiveness driver that delivers distance and accuracy. Its 460cc titanium
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535 Florida Street
San Francisco, CA 94110 ·
Office For Lease
Former Potrero Brewing Company Building
|Rental Rate
|Building Size
|Property Type
|Building Class
|Property Sub-type
|Listing ID: 17318132
|Date Created: 09/02/2011
|Last Updated: 11/21/2013
One of the most unique commercial spaces you will find in SOMA - Mission District, this property was renovated originally for a beer brewing company / full service restaurant. Inside you will find a commercial kitchen area, large windows, and open space on two levels. There is an internal stairwell and passenger elevator connecting the two levels. There are two large patio decks with sliding glass doors accessing that area. We are marketing the space to all commercial users that fit UMU zoning, including creative company tenants that want to have a large kitchen on site, an event center, or a commercial kitchen operation.
535 Florida is located within the Mariposa Square complex of buildings which comprise approximately 200,000 square feet of office and retail space. The property has a beautiful courtyard and underground parking. There is a Pilates Studio and Starbucks Coffee located in the complex. In addition, there are several well known restaurants in the neighborhood.
- Open space, high ceilings, large windows
- Full commercial kitchen with walk-in freezer space
- Two outdoor patios
- Underground parking available
- Suited for many creative uses including flex-office or a commercial kitchen operation
- Signage
535 Florida Street, San Francisco, CA 94110 (San Francisco County)
Property Record Data - Historical Listings, current tax, mortgage, owners & tenant info for this property
San Francisco Market Trends - Asking price index trends, sale price trends, asking rent trends
John-Clay Morris's Other Listings
733 Front Street, C1A
San Francisco, CA 94111
56,000 SF Building
Not exactly what you're looking for? Start a new custom search to find the perfect property
The LoopNet service and information provided therein, while believed to be accurate, are provided "as is". LoopNet disclaims any and all representations, warranties, or guarantees of any kind.


text: Designed for anglers with lots of storage pockets for organizing your gear. Tackle Gear Pack has a durable internal frame with a breathable foam back support and padded shoulder straps for comfort while carrying heavier loads. Fold-out lure storage pockets, a waterproof pouch system, padded horizontal security pocket and 10" sink compartment make it an ideal choice no matter what your storage necessities. Deluxe pack model features a padded laptop computer compartment, utility tackle trays, pockets for water bottles and large, oversized main compartment. Ergonomic shoulder pads for easy carrying. Smaller daypack model has two large compartments for books or extra layers and additional outside pockets to organize your essentials. Padded shoulder straps for comfortable transport.


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Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
Here's a quick shot of the portfolio index page design. I decided to keep with the simple is better format. Unlimited portfolio pages so that filtering isn't required or necessary, 2 column - because who wants 150 x 150 images of their work? So, nice large images that once clicked (or title or read more is clicked) it will go through to a portfolio detail page - shot coming up later to show this.
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Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2017 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


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A big forest fire broke out, Tuesday morning, next to the Jerusalem-area community of Beit Zayit. There were no initial reports of injuries.
Six firefighting crews from Jerusalem and Beit Shemesh were sent to battle the blaze, with support from two firefighting aircraft.
Tags:Jerusalem, Beit Shemesh, Fire Fighting, Beit Zayit


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We provide you with all the information you need in just one search. Plan and book every single step of your trip with just one click.
All prices include taxes and fees. There is not extra charge based on the payment method you decide to use.
Need help? Our support team is here to follow you from the very beginning to the moment you leave for your trip.
Travel from Lissone to Piacenza and discover Emilia-Romagna city of 93.2 Thousand inhabitants. The distance from Lissone to Piacenza is 73.1 kilometers; with Wanderio you can compare the available treno so that you can organize your trip choosing the fastest route, the most comfortable or the cheapest one. For example, by choosing a treno from Lissone to Piacenza, you'll get to your destination in about 1 hour on average; the average treno ticket prices are 7 €.
Compare flights, trains, buses and transfers to get from Lissone to Piacenza
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You can get from Lissone to Piacenza by train, thanks to the 28 daily routes provided by Intercity, Regionale Veloce, Frecciabianca, Regionale, InterCityNotte. The train ride from Lissone to Piacenza takes about 1 hour on average and the average train ticket is 7.3 €. Travelling Italy by train can often be cheap.


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Bonbrook Plantation is a master-planned community of new homes in Rosenberg, TX, minutes from Brazos Town Center and Sugar Land. With an array of amenities, you can choose from one- or two-story, energy efficient homes offering up to 5 bedrooms & 3.5 baths.
8802 Synder Farm LaneRosenberg, Texas
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Mon-Sat 10am-6pm
Sun 12pm-6pm
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The Fed recently raised interest rates, and experts predict three more hikes in 2017. If you wait to buy until after rates rise again, you'll pay the price for the next 30 years. Lock in a historically low rate and payment on a new K. Hovnanian® Quick Move-In Home.
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Equal Housing Opportunity | Terms & Conditions | © 2017 K. Hovnanian® Companies®, LLC.


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San Juan Hospital & Clinic - Monticello
Organization Name: San Juan Health Services
Address: 380 West 100 North
Monticello, UT 84535
Monticello, UT 84535
Phone: 435-587-5054
Fax: 435-587-3004
Service Categories:
Services Provided: San Juan Health Services District includes San Juan Hospital, Clinics in Blanding and Monticello. Additional space, current technology and remodeling have kept the physical facility and equipment current and capable of meeting today's healthcare needs.
|Original Source:
|United Way 2-1-1
|Last Updated:


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Recent Comments
Sherry D'Ambrosio Tue, 06/04/2013 - 13:38
That is the sweetest little face! The layout sets her off quite well.
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About Me
I love to design- anything and everything. I love to create. I am not very good with photoshop and wish I had more time to play!! I found Elements easier and loved the program. I have my own website where I display digital stamps, card kits, scrapboooking elements and anything I am currently working on.


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Seized cars to be sold at auction. Photos courtesy A.J. Willner Auctions. Owners can auction off cars for any number of reasons: to turn a profit on an investment, to raise funds for another vehicle purchase, or, perhaps, simply because the vehicle can no longer be driven in the desired manner. Som ... Read more
Shelby G.T. 500 KR. Photo by Eric English. No shortage of attention is given to the mechanical heroes created under the guidance of Carroll Shelby during the heyday of his Shelby American operation and its collaborations with Ford Motor Company. Yet, despite the scrutiny applied to these machines y ... Read more
1973 Ford Econoline Custom 100. Photos courtesy R. Gilligan Auctions. Of the various lots from the Larry Brown estate sale that took place last weekend, none got more attention than Brown's 1969 Shelby G.T. 500, a 9,000-mile original that – like the rest of the vehicles in the estate sa ... Read more
1969 Shelby G.T. 500. Photos courtesy R. Gilligan Auctions. It probably would have been very interesting to talk with Larry Brown about the vehicles he bought during the late 1960s and early 1970s, to hear about his preferences in them and the experiences he had. Unfortunately, Brown died during th ... Read more
Bill Cook doesn't say how old he was when he and his father stopped by Ed Martin Ford in Indianapolis on the way to the Brickyard in May 1969, but he was obviously enthused about the chance to get behind the wheel of Ed Martin's 1969 Shelby G.T. 500, as recounted on his Flickr page full ... Read more
Former Shelby American designer John Chun. Photo courtesy John King. John Chun, an industrial designer who began his automotive career with the "dream job" of sketching design proposals for Shelby American, died on Saturday, July 6. For years, Chun was known to friends and neighbors onl ... Read more
1967 Shelby G.T. 500 Super Snake. All photos courtesy Mecum Auctions. The one-of-one built 1967 Shelby G.T. 500 Super Snake may never have been, except for a conversation between Carroll Shelby and car sales executive (and former Shelby American employee) Don McCain. McCain suggested that there was ... Read more
One-of-one 1967 Shelby G.T. 500 convertible. Photos courtesy Concours d'Elegance of Texas. When it comes to exclusivity, it's hard to argue with one of one built, and most exhibition events are happy to display a single example of such a vehicle. Not so with this year's Concours d ... Read more
Automobiles and the U.S. Post Office have had a relationship since the first editions of the America on the Move series were released in 2005. Designed by Carl Herrman and illustrated by artist Art Fitzpatrick, that five-piece '50s Sports Cars collection consisted of the 1953 Corvette, 1954 K ... Read more
Photos courtesy Mecum Auctions. One hundred seventy miles per hour might be nothing for a modern supercar, but in 1967 not every car could lay claim to that speed. One that could, the one-of-one Shelby G.T. 500 Super Snake, will cross the block this spring. As the story goes, the Super Snake projec ... Read more


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Your grand-father taught me the politics of pride, your grandmother taught me the politics of poverty. I am beholden to both for the fine synthesis. To you, my darling daughter, I give only one message. It is the message of the morrow, the message of history. Believe only in the people, work only for their emancipation and equality. The paradise of God lies under the feet of your mother. The paradise of politics lies under the feet of the people.Zulfikar Ali Bhutto
In talking, shyness and timidity distort the very meaning of my words. I don't pretend to know anybody well. People are like shadows to me and I am like a shadow.Gwen John
Peace is not a relationship of nations. It is a condition of mind brought about by a serenity of soul. Peace is not merely the absence of war. It is also a state of mind. Lasting peace can come only to peaceful people.Jawaharlal Nehru
Old people have fewer diseases than the young, but their diseases never leave them.Hippocrates
As part of the spring ritual of National Poetry Month, poets are symbolically dragged into the public square in order to be humiliated with the claim that their product has not achieved sufficient market penetration and must be revived by the Artificial Resuscitation Foundation (ARF) lest the art form collapse from its own incompetence, irrelevance, and as a result of the general disinterest among the broad masses of the American People. The motto of ARF's National Poetry Month is: "Poetry's not so bad, really."Charles Bernstein
The safety of the people shall be the highest lawMarcus Tullius Cicero
The nice thing about being a celebrity is that when you bore people, they think it's their fault.Henry Kissinger
Abundance ~ An endless supply of water, trees heavily laden with fruit, a sun that shines every day, seasons in their never-ending cycle, a constant supply of air, an infinite wellspring of love, so many people to share with, an eternal cascade of imagination and creativity, unlimited lessons and growth, much valuable work to do, limitless potential for expanding and opening our hearts, unending forests to wander, evermore books to read and write, a vast mind of thoughts to explore, overflowing goodness, exponential wonder, overwhelming mercy, ~ a copious abundance of abundance to ponder.Jinjee and Storm Talifero
A secret of abundance is to stop focussing on what you do not have and shift your consciousness to an appreciation for all that you do have No limit people are so in charge that theycan trust their instincts, be childlike, be creative and do anything that makes sense to them.unknown
When you accustom people to believe that priests and clergy can forgive sins... you will have sins in abundanceunknown
People with a scarcity mentality tend to see everything in terms of win-lose. There is only so much; and if someone else has it, that means there will be less for me. The more principle-centered we become, the more we develop an abundance mentality, the more we are genuinely happy for the successes, well-being, achievements, recognition, and good fortune of other people. We believe their success adds to...rather than detracts from...our lives.îStephen R. Covey
It was not a fashionable place, but even among the pleasant people there, the girls made few friends, preferring to live for one another.Alcott, Louisa May
Simple, sincere people seldom speak much of their piety. It shows itself in acts rather than in words, and has more influence than homilies or protestations. Beth could not reason upon or explain the faith that gave her courage and patience to give up life, and cheerfully wait for death. Like a confiding child, she asked no questions, but left everything to God and nature, Father and Mother of us all, feeling sure that they, and they only, could teach and strengthen heart and spirit for this life and the life to come. She did not rebuke Jo with saintly speeches, only loved her better for her passionate affection, and clung more closely to the dear human love, from which our Father never means us to be weaned, but through which He draws us closer to Himself. She could not say, "I'm glad to go," for life was very sweet for her. She could only sob out, "I try to be willing," while she held fast to Jo, as the first bitter wave of this great sorrow broke over them together.Alcott, Louisa May
When a community is composed of honest, sober and virtuous people, your forming a bad opinion about anyone of its members, when nothing wicked has been seen of him, is a great injustice to him. On the contrary in a corrupt society to form good opinion of anyone of them and to trust him is to harm yourself.Ali bin Abu-Talib


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I am a first time home buyer and put an offer on a home. My real estate agent said I need to find a lawyer. I have no idea what this would cost. How do I know if the attorney's fee's are reasonable? I think this closing will be a typical closing with very few, if any, problems.
The best way to find out what a reasonable fee is for a buyer in your area is to ask your Real Estate Agent or your Lender to provide you with three names of attorneys that they know of who practice in this area. Since each of them would be working with Lawyers on a regular basis, they should know to whom to refer you. Alternatively, ask friends or colleagues who have bought property for a referral.
Call each attorney and ask their fee for a Buyer closing - giving as much detail as you can (Single family home or condo, date of closing, inspection issues of which you are aware, etc.). Then ask who will handle your file - will it be the attorney you are speaking to, an associate, a paralegal, a secretary? Don't commit until you have done your research.
You shouldn't look for the cheapest price - you should look for the attorney with whom you have a rapport and whom you feel will give you the best representation. You might also want to look to see what they say on their website and if they maintain an AVVO profile, so you have some idea as to their qualifications.
This answer does not constitute legal advice and does not and is not intended to create an attorney-client relationship. The law may vary depending on the state in which you reside. It is intended only to give some direction in which to seek assistance.
Dear Toms River,
I generally charge $850.00 for a purchase.
Aniello D. Cerreto, Esq.
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Journey through Lent with the best devotional writing of pastor and martyr Dietrich Bonhoeffer. Click to sign up.
These concluding exhortations, very like what one finds elsewhere (e.g., 1 Cor 16:13; 2 Cor 13:11; 1 Thess 5:12-24), are tailored to the situation in Philippi. Among other things they are to rejoice in the Lord, to let their "gentle forbearance" be evident to all (including those who oppose them) and to not be anxious about anything (given the present opposition and suffering), but let prayer and thanksgiving lead them to experience God's peace. The exhortations fall into two clear parts: verses 4-7, in which Paul appropriates his Jewish heritage, and verses 8-9, which reflect both his Hellenistic Jewish background (Jewish wisdom) and the best of Greco-Roman philosophical virtues.
The heart of the first set reflects the threefold expression of Jewish piety—rejoicing in the Lord, prayer and thanksgiving—which are basic to the Psalter: "the righteous rejoice in the LORD" (Ps 64:10; 97:12) as they "come before him with thanksgiving" (Ps 95:2; 100:4) to pray in his sanctuary (Ps 61:1-4; 84:1-8). For Paul these are the work of the Holy Spirit in the life of the believer and especially of the believing congregation. They are expressed as imperatives because, in keeping with the Old Testament, devotion and ethics are inseparable responses to grace. The truly godly person both longs for God's presence, where one pours out one's heart to God in joy, prayer and thanksgiving, and lives in God's presence by "doing" the righteousness of God. Otherwise piety is merely religion, not devotion.
Also in keeping with the Psalter, these (second-person plural) imperatives exemplify the conjunction between individual and corporate piety (see commentary on 2:4 and 13). For Paul joy, prayer and thanksgiving, evidenced outwardly by gentleness (v. 5) and inwardly by God's peace in their midst (v. 9), have first to do with the (gathered) people of God; but the fact that God's peace will serve as a garrison for your hearts and minds reminds us that what is to be reflected in the gathered community must first of all be the experience of each believer.
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This review is from: Dell P2312H Black 23" 5ms Swivel , Height & Pivot Adjustable Widescreen LED-Backlit LCD Monitor 250 cd/m2 DC 2 Million:1 (1000:1)
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More than half a century ago this week, August 12, 1958, some of the greatest jazz musicians of the day assembled in Harlem at what for them was pretty ungodly hour - 10 A.M. Fifty-seven jazz-makers came to East 126th Street to have their picture taken for Esquire magazine. Art Kane, the photographer, bunched them together in front of the steps of two brownstones. Some neighborhood kids plunked down down the curb and so did the pianist-bandleader Count Basie. And A Great Day in Harlem was captured in a black and white image. NPR's special correspondent Susan Stamberg says there were just three women in the photo, and now one of them is the subject of a documentary film.
SUSAN STAMBERG, BYLINE: It's Marian McPartland, beloved by fans of NPR's long-running Piano Jazz program. In the 1958 Esquire photo, she's wearing a halter dress like the one Marilyn Monroe wore when she stood over that up-windy subway grate. McPartland's dress sits flat and proper. The 94-year-old jazz pianist remembers the day that legendary photograph was taken.
MARIAN MCPARTLAND: I was working at the Hickory House and Nat Hentoff came rushing in and said you've got this date to have a picture taken 10 o'clock. And I didn't particularly want to get up that early but I did. And as you know, it turned out to be a fabulous photograph.
STAMBERG: So fabulous that in 1994 a documentary film was made about it.
UNIDENTIFIED MAN: This film is the story of a magic moment when dozens of the greatest jazz stars of all time gathered for an astonishing photograph.
STAMBERG: The picture shows Dizzy Gillespie and Thelonius Monk and Charles Mingus, Gene Krupa and Maxine Sullivan and Mary Lou Williams and Gerry Mulligan and Lester Young and Sonny Rollins - all the '50s gods of jazz.
STAMBERG: And Marian McPartland.
MCPARTLAND: I never get tired of looking at that picture, one of the world's greatest photos.
STAMBERG: Well, I'm just looking at you there and you stand out, as you must have, when you first went to New York. Because you had three things that could have gone so against you; you were a woman, you were white, and you were foreign. It occurs to me you had three things that were so in your favor. One was your enormous talent and, two, your great charm, and, three, you were some beautiful young woman - and still are, of course.
MCPARTLAND: Well, trying to be in my old age. It seems like everybody encouraged me and let me be myself. Nobody bothered whether I was black or white. I just wanted to play better and listen to a lot of people, people I really loved - Bill Evans and horn players like Sonny Rollins. And, oh, I just wanted to hear everybody.
STAMBERG: Now, there's a documentary film about Marian McPartland. "In Good Time: The Piano Jazz of Marian McPartland" is full of archival footage, family photos, interviews. A fellow named Huey - one name only - spent five years making the movie.
HUEY: I was very lucky. There's, oh, so many wonderful moments that I found. And one of the precious ones is the show where Jimmy McPartland, Marian's husband, and Marian are on together and talk about how they first met, which was a real gem to find.
JIMMY MCPARTLAND: How we met?
UNIDENTIFIED MAN: Yeah.
MCPARTLAND: The concert - first thing, they had a big jam session there. You know, they were having a big session. Jimmy McPartland's coming, Jimmy McPartland's coming, and I'm saying, you know, who's Jimmy McPartland?
STAMBERG: Jimmy McPartland was a famous jazz cornet player.
MCPARTLAND: I asked what do you want to play? Marian had said yes. She said, you know "Honeysuckle Rose?" I said, oh, yes, I know the guy that wrote it. Fast forward, yeah, and asked what should we do? Play. I said, OK. So, I beat off and stamp, beep-bo-ta-do-po, beep-ta-da-tat. She started the de-do-do-da-da and then she started to get excited. Den-ta-da, da-dat-da-da. And she's getting excited. And rushing, you know, and I, oh no. This is it, you know. But...
MCPARTLAND: Yes, you were right.
He was foot soldier and I was working in USO camp shows. And somehow we got together over there and we were married in Achen, Germany. So, we were married by the time we got back here to Chicago. It's Jimmy's hometown. And it took a while for us to uproot ourselves and move to New York, 'cause that's where things really happened more. And Jimmy found a gig for his band. And I was at the Hickory House, so we were all busy.
MCPARTLAND: It's just a very open kind of club. People just walked in. A lot of times, they would just jump on the bandstand and sit in with me. It was a ball.
STAMBERG: And I know that Duke Ellington came every night - he was a fan of yours - but I'm not sure he was coming every night just to hear you play.
MCPARTLAND: Well, I don't know. He probably came because the food was good. And his press agent was also the press agent for the Hickory House.
STAMBERG: Will you tell what it was that Duke Ellington said about your piano playing?
MCPARTLAND: Oh, I remember he made a sort of a subtle criticism. He said, oh, you play so many notes. I thought he's obviously telling me I'm playing too many notes. So, from that I kind of eased off a little bit in that I was probably showing off.
MCPARTLAND: Hi I'm Marian McPartland. My guest today on Piano Jazz...
STAMBERG: All those notes found a home for 30 years on public radio. Marian McPartland's Piano Jazz began in 1979. The first program was recorded in the Baldwin Piano showroom on 59th Street in New York. There was Marian, surrounded by pianos for sale, just playing away. Some 700-plus programs later - her last show was recorded in 2010 - the series keeps running and running and running - archival tapes, plus new ones, with new hosts. Marian remains the show's artistic director. Her radio guests over the years have included all the jazz greats: Billy Taylor, Bill Evans, Sarah Vaughan, Norah Jones, Oscar Peterson, George Shearing, Herbie Hancock, Ray Charles, Tony Bennett.
STAMBERG: There is moment in the film which brought me to tears, and it was you and Dave Brubeck.
MCPARTLAND: Oh, yeah.
STAMBERG: When he's playing a song he wrote for you based on rhythms of your name.
DAVE BRUBECK: Ma-ree-an Mc-part-land.
AUDIENCE: Ma-ree-an Mc-part-land.
MCPARTLAND: We managed to get three or four choruses out of it. We had fun. I know we were laughing with one another.
STAMBERG: I'd love to talk about your relationship with Diana Krall, because that's been a wonderful relationship over the years, hasn't it?
MCPARTLAND: Yes, it has. When I first met her, she was a very young girl. And she called me on the phone and left a message.
DIANA KRALL: I came home one day and my dad said Marian McPartland phoned. What are you doing calling Marian McPartland? I said, well, I needed, I wanted to ask her for advice on what she did, how did she do what she did. I just wanted to talk to her, 'cause she's the only woman jazz pianist I know of.
MCPARTLAND: I called her back and she seemed sort of surprised. We got along famously, it seems, right away. And I follow her and all her doings.
MARIAN MCPARTLAND AND DIANA KRALL: (Singing) There is nothing I couldn't do if I had you, if I, if I had you.
STAMBERG: A duet between friends - Diana Krall, accompanied by Marion McPartland - on "Marian McPartland's Piano Jazz." Krall, Brubeck, so many others appear in the new documentary film, "In Good Time: The Piano Jazz of Marian McPartland". It will be screened in the fall at jazz festivals in Savannah and Seattle. You can see the trailer for it at nprmusic.org. Ms. McPartland spoke to us from her home in Port Washington, New York. Thank you, Marion McPartland so much.
MCPARTLAND: Oh, it's a pleasure, Susan.
STAMBERG: Pleasure to talk to you. I'm Susan Stamberg, NPR News.
SIMON: This is WEEKEND EDITION from NPR News. I'm Scott Simon.
Copyright © 2012 NPR. All rights reserved. Visit our website terms of use and permissions pages at www.npr.org for further information.
NPR transcripts are created on a rush deadline by Verb8tm, Inc., an NPR contractor, and produced using a proprietary transcription process developed with NPR. This text may not be in its final form and may be updated or revised in the future. Accuracy and availability may vary. The authoritative record of NPR's programming is the audio record.


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Gefilte Fish
|3 Pound
|Yellow onions/2 small
|1 Large (Peeled)
|Matzo meal
|3⁄4 Cup (12 tbs)
|Cold water
|1 Cup (16 tbs)
|1 Teaspoon
|Fish stock
|1 Cup (16 tbs)
|To Taste
|To Taste
Put the fish and onion through a meat grinder, or have the fishmonger grind the fish for you.
Place the mixture in a bowl, add the other ingredients, and mix thoroughly.
Do not use all the matzo meal at once because you may not need much in order to make a firm and workable fish paste.
Season generously with salt and pepper.
Form into balls, and cook in the fish stock.
Let come to a boil; then cook slowly for 1 hour with a lid on the kettle.
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 2620 Calories from Fat 575
% Daily Value*
Total Fat 64 g98.1%
Saturated Fat 12.7 g63.6%
Trans Fat 0 g
Cholesterol 1344.4 mg448.1%
Sodium 1903.9 mg79.3%
Total Carbohydrates 178 g59.5%
Dietary Fiber 13.3 g53%
Sugars 6.2 g
Protein 315 g629.5%
Vitamin A 46.7% Vitamin C 0.39%
Calcium 27.1% Iron 73.6%
*Based on a 2000 Calorie diet


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Luke Kenneth Casson Leighton <l...@samba-tng.org>
|Re: cvs commit: apr/threadproc/unix thread.c
|Thu, 07 Jun 2001 14:07:50 GMT
> > See, that's where my overall view of "where we hope to get to" differs. > > <shrug> In my mind, APR depends on pools. Period. It would require a > > major overhaul for most APR operations to be safe WITHOUT pools (ie, lots > > of apr_sms_free operations would have to be added, which is exactly what > > the pools are meant to avoid). no, no, _wrong_! that's exactly what you _don't_ do, and anyone who proposes it, or thinks that that is what is being proposed, i will stamp on their fingers or break their keyboard in frustration. or something. no. we are _not_ proposing that pools be replaced [with something other than what looks exactly like pools]. same API [pools], different implementation. doing otherwise is just.. _nuts_! you know that, _i_ know that. what on earth makes you think _i_ think otherwise??? darn :) luke
|View raw message


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As so many managers of so many different abilities have before him, Fabio Capello sought reassurance from Sir Alex Ferguson this month.
The England manager wanted to know if Phil Jones was capable of playing in central midfield at international level and whether, on top of that, he was capable of doing so against the world champions Spain.
Capello related the nature of his conversation with Ferguson yesterday as if to prove a point. "He [Jones] was in my mind when I met him [Ferguson] in Manchester [last week for the celebrations for Ferguson's 25th anniversary as United manager]. He [Ferguson] said he could play there. You know, last year he [Jones] played in midfield."
It was instructive that even for a fellow manager such as Capello, who is just four years Ferguson's junior and is one of the few managers alive with a record that even gets close to comparing, the word of the Scot is taken with such solemn assurance. Because, make no mistake about it, playing Jones against the best midfield in the world today is a risk. It is an intriguing, beguiling risk. One that may even turn out to be inspired. But a risk, none the less.
Jones will feature in holding midfield this evening, in all probability alongside Scott Parker, with Frank Lampard just ahead of the two, against what is the greatest international midfield of the modern era. Xavi Hernandez and Andres Iniesta are daunting opposition but Capello, like every international manager, is essentially a man in a hurry. He does not even feel that he can wait until Tuesday and the game against Sweden to give Jones his chance.
When it was put to Capello – not entirely seriously – that he should bear in mind when taking advice on the England team that Ferguson is Scottish, he laughed. But he appeared more amused at the memory of having seen Ferguson wearing a kilt than any suggestion that he might have been given a duff steer from a patriotic Scotsman.
Jones starts today, just his second England cap, in midfield against the world champions. But who are we to laugh at Capello's eccentricities when he has to endure so many of ours? This week the England manager has already seen his team at the centre of a row over poppies, his captain is the subject of a police investigation and the announcement of the team hotel for Euro 2012 basically came down to the question of where the WAGs would be staying.
Compared to all that, the fact that Capello's own son would be absent-minded enough to schedule his wedding for an international weekend seems relatively mild in comparison.
In a nutshell, Capello said yesterday that he cannot afford to treat today's game as if it is a match that must be won at the cost of trying out new players and different formations. He has a limited number of opportunities to prepare for Euro 2012 – and the three games without Wayne Rooney (pending an appeal) – and there is no time to waste.
It was with a degree of exasperation that Capello explained his position. "Guys, for me, these are important games [in which] to see the value of the players I have to select. These are difficult games. Spain and Sweden have different styles, completely different styles, and then the Dutch to come [in March].
"I want to understand what will happen with the players I select when we go to play the really important games at the Euros. Those matches at the Euros will be more difficult. So I need to know the value of the players. Also, these three games, we will play at home with the pressure. Here, the pressure is more than away from home. For this reason, you can understand the value of the players [better] here, than away."
In the case of Jones, Capello was effusive in his praise, even mentioning Franco Baresi in the same sentence as the 19-year-old. To clarify that point he said that Baresi, the old defender who was a mainstay of his first Milan team, was a better defender than Jones; but Jones the better midfielder.
As for the United teenager, Capello said: "He can play right-back, he can play centre-back, he can play, for me, as a midfielder because he's really a fantastic player. But I know him as a right-back, where he's really good. He played really well against Montenegro. I saw him twice as a central defender, at centre-back. I remember him as a central midfielder. I want to see him there in a really important game against Spain.
"I think he's got a lot of qualities, really good qualities. His potential is the top. The top. You need quality to be able to do that. When he goes forward, when he reaches the last metres near the box, his passing is really good. He can shoot. He can [get close to] the goal. I think so."
Without Rooney, Steven Gerrard, Jack Wilshere and Rio Ferdinand there is little more that Capello can do but try to make some changes and put a brave face on it if things work out badly today. There is much to be said in playing Jones – what would another cap for Gareth Barry add to Capello's understanding of this team? – the only real fear being that if it is a bad experience it may set the player back.
Otherwise, this is a team that will probably spend much of the game defending in a 4-5-1 formation. That leaves Capello with a dilemma over his striker. Darren Bent is more likely to seize on a half-chance that earns his team a goal but Danny Welbeck is a more obvious candidate to occupy the Spain defence. James Milner's form should earn him a start on the left with Theo Walcott on the opposite wing.
If anything, Jones's selection shows that England have, to some degree, moved on from the team that was eliminated by Germany in South Africa in July last year. Then the teenager would scarcely have elicited a flicker of recognition from Capello. Today he is starting against a team for whom the Italian can barely conceal his admiration.
Asked whether he was doing the right thing, Capello held his ground. "It's my job, guys. It's my job," he said. "I have no fear. Nothing. No fear. Yes, I think the team that will play [today] will be really, really, good." He sounded like a man who had weighed up the benefits of playing it safe and had decided, this time at least, to go for broke.
Defenders in midfield a good idea?
Is Fabio Capello right to play defender Phil Jones in midfield? Here are some cautionary tales:
Gareth Southgate
A great man-manager but never regarded as a master tactician, Kevin Keegan made a mistake in deploying Southgate in the holding role in the 2002 World Cup qualifier against Germany in the final game at the old Wembley in October 2000. It was far from Southgate's finest display, and eventually he was moved back into a safer central defensive position. England lost 1-0 thanks to Dietmar Hamann's free-kick, and Keegan resigned after the game.
Jamie Carragher
Was first used in midfield by care-taker manager Peter Taylor in the 1-0 friendly defeat in Italy in November 2000, coming on for Nicky Butt. He played in the holding role for Sven Goran Eriksson in the 3-1 World Cup warm-up win over Hungary in 2006. His performance was competent enough but he was switched to right-back for the second half.
Ledley King
Made his first England midfield performance coming on as a substitute for Paul Scholes in the 70th minute in the 4-2 group stage win over Croatia in Euro 2004. He went on to play the holding role excellently in a 2-1 World Cup qualifying win against Poland in 2005, starting in a 4-1-3-2 formation. However, he struggled a month later tracking the runs of Juan Riquelme in the 3-2 friendly win over Argentina.
Tom Sweetman
- More about:
- Europe
- Fabio Capello
- Premier League
- Sir Alex Ferguson
- Spain


text:
Western Sydney Academy of SportSports Cycling Clubs
Station St, Penrith, NSW 2750
Best match results for: CATEGORY = Bikes & Cycling Activities for Kids + LOCATION = Hawkesbury
Results include related kids activities and neighbouring locations.
Bikes & cycling activities for kids around Hawkesbury
Station St, Penrith, NSW 2750
31 Darling St, Abbotsbury, NSW 2176
Old Castlereagh Rd, Emu Heights, NSW 2750
Sport activities for kids in Hawkesbury
Tusculum Rd, Valley Heights, NSW 2777
Rickards Road, Warrimoo, NSW 2774
358-360 Hawkesbury Road, Winmalee, NSW 2777
Hawkesbury Rd, Winmalee, NSW 2777
Hawkesbury Road, Winmalee, NSW 2777
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Sport activities for kids around Hawkesbury
SPRINGWOOD, BLAXLAND, HAZELBROOK,LEURA, WINMALEE, Springwood, NSW 2777
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I sent my kids to mountain wolves tkd and they love it they have learned so much about self defence, discipline and heaps more and thats just in one term id recommend this place to every body doesn't matter skill or age i love it.
St Margaret's Church, 15A Wilson Way Blaxland, Blaxland, NSW 2774
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This business offers products or services online.
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3 Windswept Ln,Mansfield CT, 06250
4 beds , 3 Full baths
2,600 sq. ft
Status: Active
Berkshire Hathaway HomeServices New England Properties
(855) 295-8440
Last Updated: 2/25/2017 9:23 PM
Description for 3 Windswept Ln Mansfield, CT 06250
This 2.76 Acre lot is only 1 Mile from UCONN, stores, shopping and major highways. This beautiful lot borders 153 acres of UCONN Plant and Science division. It also borders 6 acres of Conservation easements that makes this lot the perfect spot for your dream home. Bring your plans or use ours, many to choose from. Don't miss out on this beautiful lot. Property is Broker owned. Specs can be customized to suit your needs. The house plans are one example of what can be built. Buyers are welcome to adjust any plans available or add their own features.
Listing Information
Storrs (54)
Well Private
Cable TV, Propane
School Information
Room Information
Interior Features
Central Air
Forced Air
Full, Unfinished Basement
Internet Connection
Exterior / Lot Features
Attached Garage, Asphalt, Paved Driveway
Deck, Patio, Porch
Level, Open
Financial Considerations
Inside AccessTM
Current market conditions for 3 Windswept Ln
Active Market Trends
Source:MLS DATA
Median list price$272,400
Days on market193
Properties for sale66
Sold Market Trends
Source:MLS DATA
Median sold price$200,000
Sales to list price9,999.0%
Properties sold55
Median sq ft price$111
Arrows indicate 90 day trend
Listing Price History for 3 Windswept Ln
Mansfield Real Estate
Mansfield is located in Connecticut. Mansfield, Connecticut 06250 has a population of 26,182.
The median household income in Mansfield, Connecticut 06250 is $67,615. The median household income for the surrounding county is $80,887 compared to the national median of $53,046. The median age of people living in Mansfield 06250 is 21.3 years.
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(855) 295-8440
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Speed & Explosive Power
Speed Agility Quickness
Combine / Showcase Prep
Recruitment Assistance
Every time my son comes home from your place he's in the most upbeat mood. You've really helped his confidence as a young man. Thank you.
Thank you for pushing me all these years and not allowing me to settle for anything less than my best!
4 Time Collegiate All-American
Love DIPT! They are the best out there. All custom workouts.
Proud Parent
Her coaches couldn't believe the progress she made...
M. Wilson
Monday - Thursday
6:00am - 9:00pm
6:00am - 7:00pm
7:00am - 4:00pm