text:
Consumers Approve User Generated Commerce
We know that user generated content influences consumers to buy products more effectively than advertisements do. At Fashion Digital NY we met fashion, technology, inspiration, and trends blogger, Alice Chan. Anytime I post about a person it's about someone that influenced me and inspired me. LG: Has user generated content influenced you to buy a product?
Fashion Influencer Marketing: What Good Looks Like
social influencer is someone who uses a social network like Facebook, Twitter, Pinterest, Instagram, YouTube, or other sites to deliver opinion pieces or feedback about a particular product. How many Facebook posts, news articles or blogs have you seen that start with "This person is wearing a stunning X to the Met Gala?" User-Generated Content (UGC) and Social Influencer Marketing.
Social media giants tighten their grip on small content producers
grow - Practical Marketing Solutions
Youtube's your place to be. Popular social media platforms have tightened their grip on user generated content. A few years ago, Facebook limited your business/fan page reach in exchange for payment. The newest social media giant to limit one's content marketing is the video platform — and second biggest search engine — YouTube. Start a podcast.
10 soul-grabbing brands that prove you don't need SEO to succeed
grow - Practical Marketing Solutions
And if you're Google, it's worth comparing the short-term upside of strangling the best (thoughtful, personal, informed) content to the long-term benefit of creating a healthy ecosystem. Often, content moves on the web simply because a person or brand is powerful. We all have personal brands but we don't all have Heroic Brands and clearly Seth Godin is a Heroic Brand.
11 Ways Influencer Marketing Can Increase Your Brand's Audience
From someone telling their friends and family members about a brand on Facebook to a YouTube personality with a large following sharing product reviews, influencer marketing is both prevalent and pertinent in today's market. Influencer marketing is native advertising, meaning it is part of the content users already view rather than being separate from it. Boost your SEO.
83 Exceptional Social Media and Marketing Statistics for 2014
internet users researching products and services online. ( B2B Marketing ). However, just over a third of marketers view each channel as valuable for lead generation. 24. 8% of Internet users account for 85% of online display ad clicks. B2B marketers rate in-person events and case studies as the most effective content marketing tactics (see full list below). ").
Web 2.0 and Personal Learning Environments (PLEs) " Learning Matters!
Buzz Marketing for Technology
and Personal Learning Environments (PLEs). think because of this, we were kinda able to get right into it and riff because we each knew where each other stood and we got to take the conversation into the generative learning and co-creation stage very quickly. Time is on the same channel here: First, get an account on blogger and start to converse publically. Learning Matters!
Best Web Presence Optimization Guides and Tips of 2011
One of the most interesting aspects of web presence optimization (WPO) is how frequently bloggers and journalists write about the concept without actually using the term. Krista LaRiviere (see below), a co-founder of gShift Labs , is one of the few bloggers who have embraced the term. Web Presence Optimization (WPO) Guides and Insights. The New Breed of B2B Buyer by Chaotic Flow.
The Ultimate Glossary: 120 Social Media Marketing Terms Explained
AddThis - AddThis is a social bookmarking service that provides a code users can put on their websites so that when people visit that site, they have the option to share via Facebook, Twitter, etc. Avatar - An avatar is an image or username that represents a person online within forums and social networks. B. and then provides every user with his or her Klout score. link] ). V.
Visual Content Marketing Insights from 13 Genius Thought Leaders
Humans are simply wired to process visuals more effectively, and the increasing prevalence of visually-oriented platforms like Pinterest and YouTube has only fed this demand. Using YouTube or Vine for marketing is within nearly anyone's budget , and we're inclined to believe Kozlowski that it's the direction in which content marketing is headed. 2. Sometimes, they're user-generated.
The Ultimate Glossary: 101 Social Media Marketing Terms Explained
Avatar - An Avatar is an image or username that represents a person online within forums and social networks. Bit.ly - Bit.ly is a free URL shortening service that provides statistics for the links users share online. Blogger - Blogger is a free blogging platform owned by Google that allows individuals and companies to host and publish a blog typically on a subdomain.
30 Low-Cost Ideas for Content Marketing on a Shoestring Budget
Not only are memes easy to make with the help of aonline tool like memegenerator, they can be generated in less than five minutes, and they're the epitome of low cost marketing. Pass the spotlight to other talented content creators in your niche, and save yourself some time, by creating a round-up post, sharing in infographic, or embedding a YouTube video on your website. Slideshare.
How B2B Marketers Can Succeed on the 6 Big Social Networks
So this post will break down how you can be successful on the 6 most popular social networks: LinkedIn, Twitter, Facebook, Google+, YouTube, and Pinterest. Did you know that LinkedIn is 277% more effective for lead generation than Facebook and Twitter? Why not add a call-to-action in those slides, repurpose them as lead generation content, and share it on LinkedIn? At HubSpot, we update our LinkedIn company status 4 times a day, each time publishing either a new blog post, or a lead generation offer. Take a look for yourself. 5) Generate leads from pins.
Why Niche Social Networks Like Empire Avenue Matter to Social Media Marketers
WindMill Networking
Outside of Facebook, people are spending on average more time on Empire Avenue than any other of the above leading social media websites , spending even more time on average than on YouTube: Facebook 32.3 minutes YouTube 17.8 This information on social scores, which is broken down by platform, is then given for every user. minutes Empire Avenue 25.1 minutes Reddit 10.1
Marketing Edge
Marketing Edge
Subscribe to our podcast « GM Fastlane's Bob Lutz: Keeping the faith on blogging Provident Partners increases its workforce by 50 percent » VSocial connects viral video and business objectives OK, YouTube is great for parties, vacation videos and has some utility for business, but is it the perfect platform for a company's multimedia social engagement? As we uncover with vSocial CEO and co-founder Mark Sigal, other tools on the vSocial platform let you build microsites that can be filled with user-generated content. What should I do?
How NOT to pitch a blog | Online Marketing Blog
delicious b2bmarketing
Home About Resources Archives Subscribe Consulting Contact How NOT to pitch a blog 29 Comments | Posted by Lee Odden on Aug 29th, 2007 in Blog Marketing , Blogging , Online Marketing , Online PR In the past I've written about blogger relations offering tips on how marketers or PR professionals ought to present their story ideas to bloggers. Pitching bloggers and print journalists are somewhat similar, but in the end, they can be very different things. If you can't bother to find out the name of the blogger, then your message isn't really that important.
19 New Featured Sources on the B2B Marketing Zone
The B2B Marketing Zone has really taken off over the past few months with the recent addition of numerous great bloggers. the BMZ) was officially launched a year ago as the first content aggregation hub for leading business-to-business bloggers. B2B Voices ( Trade Youtube Twitter Leads Sales ). B2B Sales Lead Generation using SMM , Monday, July 19, 2010. June 7, 2010.
Marketing Edge
Marketing Edge
REI can build a larger following in 2008 across its wide variety of constituents: campers, cyclists, kayakers and on and on. 2) The Twitter piece can tie into a microsite with a combination REI expert- and user-generated content for REI Adventure. Give us more: more personality, more views, more experience. What should I do? That's not very green. Didn't crack the code.
Marketing Edge
Marketing Edge
In the letter and the YouTube video, there was no mention of what the passengers who were so inconvenienced were to receive. Second, the YouTube video featuring JetBlue's CEO was a good use of that venue, but I think it could have been done better. Now on to the NewComm Forum and our drawing to send one lucky person to the forum in Las Vegas. What should I do? Come on.
How Social Media Is Impacting Search With Rand Fishkin [@InboundNow #22]
Getting bloggers to link to you. Leveraging user-generated content with YOUmoz. and author authority (How important is this person who tweeted it?). Getting Bloggers to Link to You. One of my favorite things that people say is 'I really want to get links from all these bloggers. How do I get links from all these bloggers?' This is the direct impact.
Marketing Edge
Marketing Edge
Internet users will consume online video by 2011, up from 62 percent last year — that's 114 million people who watched video online in 2006. Whether it's purchasing video online from iTunes, consuming viral video from YouTube, or taking in the growing number of high-end video channels like Cigar Cinema , there are as many varieties of video technologies as there are videos on the 'Net. Brightcove gave a little tremor to the user community a couple of weeks ago when it announced changes to its consumer accounts, which, as of Dec. What should I do?
Marketing Edge
Marketing Edge
Subscribe to our podcast « Multimedia search & viral video essentials YouTube gives us voice: Now will we listen? The personal stories and the emotional elements lend themselves perfectly to using new media to tell stories effectively. About Us What We Do Spotlight Case Studies Contact Us Team Experience Public Relations Social Media Consulting Digital Content Production Health Care Communication News & Updates Marketing Edge Blog & Podcast Events News & Updates Marketing Edge Blog & Podcast Events Dear Provident Partners, I have a problem.
100 Social Media & Content Marketing Predictions for 2010
Junta 42
With shoot-and-upload video getting easier and easier, everyone's going to jump onto YouTube. believe that the next "big thing" will be a greater sense of personalization and location-based offerings. Graeme Harrison The best marketers/brands will generate great ideas, and create well told stories that are told across a variety of media channels. User expectations have changed and consumers are no longer willing to settle for mediocre experiences. They'll see it as a way to engage consumers and generate some publicity. Seth Godin.


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With all due respect, don't you Alden gents think the 'nephew - uncle' thing is kind of creepy?
post #40591 of 122416
1/8/13 at 6:43am
This question always seems to create some entertaining fireworks.
Maybe the Shoe Mart. Probably not in stock, but they will list those sizes on their website as sizes they can order.

subset: commoncrawl


text:
Broiled Whitefish
|Whitefish fillets
|8 Ounce
|Vegetable oil
|1 Tablespoon
|1⁄4 Teaspoon
|Lemon wedges
|Parsley sprigs
|To Taste
|To Taste
1) Debone the fish fillets.
2) Gently run your fingers across the fillets from head to tail to raise the bones, then use needle-nose pliers to pull out the bones.
3) Rinse the fillets and pat dry with paper towels.
4) Preheat the broiler.
5) Grease a baking sheet and brush the fillets with oil.
6) Season the fish with salt and pepper.
7) Broil 8 to 10 minutes until opaque throughout.
8) Sprinkle the fish with paprika and parsley.
9) On a serving platter arrange lemon wedges and place the fish fillets and serve.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size
Calories 111 Calories from Fat 64
% Daily Value*
Total Fat 7 g11%
Saturated Fat 1 g5.1%
Trans Fat 0 g
Cholesterol 34 mg11.3%
Sodium 128.4 mg5.4%
Total Carbohydrates 0.77 g0.26%
Dietary Fiber 0.38 g1.5%
Sugars 0 g
Protein 11 g21.9%
Vitamin A 5.2% Vitamin C 7.7%
Calcium 1.9% Iron 1.9%
*Based on a 2000 Calorie diet


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Breaking News:
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Project Breakthrough
I believe that you and I wish the same thing, just like everybody in the Online Marketing world.
Business Freedom meaning – never ever have two turn my bank account upside down and wishing that I had
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what you want to do every day. You have your own agenda and you have passive income streams coming
in both when you sleep and when you are celebrating a luxury vacation with your loved ones.
Now if Business Freedom is equal to "Project Breakthrough" – would you believe me? Probably not.
Project Breakthrough – 14 Day Online Marketing Training at No Cost For You
If I told you that Project Breakthrough isnot costing you a cent…
It´s a 14 day Online Marketing training carried
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idea to teach newbeees in the Online Marketing World on how to earn their first commission ever. The interest was
overwhelming and it did not take long to understand that this was something everyone wished to go through, newbie as experienced!
To make the the training meaningful, not only for teaching you on how to build a business in any
trade, an Income Stream was designed. It turned out to be 28 Passive Income Streams right now and a Back End with a possibility to a
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If I were you, I´d give it a try!
Talk soon
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Andiamo outdoor pizza oven
The Andiamo portable outdoor pizza oven is a family of fully-assembled pizza ovens with a metal enclosure and stand on casters that are easy to move. These wonderful pizza ovens deliver a true Italian wood-fired cooking experience where you want it. Featuring an attractive metal gabled enclosure, stand, and locking casters, the Andiamo portable pizza oven requires zero set-up and they can be easily rolled into place—whether it is onto a patio, or out of the garage for a mid-winter pizza party or roast turkey.
Like the other Forno Bravo assembled ovens, the Andiamo Series takes advantage of high-tech refractories and space-age insulators to deliver outstanding cooking performance and durability. The Andiamo pizza ovens provide the ultimate balance of being big enough for serious cooking—while staying small enough to be easily moved where you want it.
The Andiamo pizza ovens are available in two sizes: the Andiamo60 offers a 24" cooking floor (drawing), while the Andiamo70 delivers more cooking space with a 28" cooking floor (drawing). Both ovens features a 6" oven landing, which is perfect for keeping food warm, and for resting pots and roasting pans.
The Andiamo outdoor pizza oven is the right choice for homeowners who do not have the space, time or budget for a larger wood fired oven, but who still want to enjoy authentic wood fired cooking, Vera Pizza Napoletana and hearth bread.
The ovens include a traditional Italian-style vent and stove pipe flue. The flue is essential to proper oven operation, allowing efficient heat movement for authentic high-heat cooking. On a practical note, it keeps hot air and smoke away from the chef, and keeps the front of the pizza oven less sooty.
The Andiamo outdoor pizza oven has an attractive and weather-proof enclosure, stand, and casters comes fully assembled, so there is nothing to set up. You just roll it out of the shipping crate. And the enclosure and stand are both powder-coated for years of rust-free use.
The Andiamo outdoor pizza oven combines fast heat-up (as quick as 20 minutes) with excellent heat-holding capabilities. The outer shell of the oven stays cool, while the pizza oven chamber easily maintains 750ºF plus, to bake authentic Italian pizza in two minutes.
The Andiamo outdoor pizza oven includes a steel door with a thermometer. Optional accessories including pizza peels, an oven brush, and infrared thermometer sold separately.
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text:
Many natural products with antibiotic, anticancer and antifungal properties are synthesized by non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), yet most microbes do not express the great majority of their NRPS or PKS enzymes. In the first granting period of this R01, we developed mass spectrometry (MS) into a facile approach to directly detect covalent intermediates in ~30 systems that have been studied in vitro. We propose to continue this activity for peptide antibiotics and iterative PKS systems (Specific Aim 1).
In Specific Aims 2 and 3, we also propose to streamline NRPS/PKS assays using high throughput LC-MS. This blends aspects of enzymology with microbial proteomics of native producers into an entirely new approach to natural product discovery and the study of NRPS and PKS systems. By virtue of their huge size (often >2000 amino acids) and unique marker ions deriving from their common cofactor (phosphopantetheine), we show proof-of-concept for mass spectrometry-based proteomics with highly selective detection of expressed NRPS/PKS gene clusters in native proteomes - without requiring genome sequence information a priori. This method (dubbed "PrISM") will allow unambiguous detection of NRPS/PKS systems in Bacilli, Streptomyces, and even fungi. We therefore advocate a new "proteome-first" strategy to find gene clusters that are actually expressed and efficiently link them to the natural products they produce. The new approaches proposed here are especially timely in an era when new types of mega-enzyme "assembly lines" that defy classification and cloning are being discovered at an accelerating rate, but relatively few new approaches are available to study them. With emphasis on this area of natural products expanding, our activities will assist in the fundamental understanding of chemical processes involved in biogenesis of new natural products of the types that have provided so many of our societies useful drugs to fight both bacterial infection and cancer.
The discovery of new and novel natural products is burdened by the rediscovery of common antibiotics and antiproliferative compounds. Compounds made by NRPS/PKS enzymes have proven to provide diverse modes of action toward myriad different targets. The use of proteomics technology to discover natural products is a novel approach to this difficult problem.
|Doroghazi, James R; Albright, Jessica C; Goering, Anthony W et al. (2014) A roadmap for natural product discovery based on large-scale genomics and metabolomics. Nat Chem Biol 10:963-8
|Albright, Jessica C; Goering, Anthony W; Doroghazi, James R et al. (2014) Strain-specific proteogenomics accelerates the discovery of natural products via their biosynthetic pathways. J Ind Microbiol Biotechnol 41:451-9
|Chen, Yunqiu; McClure, Ryan A; Zheng, Yupeng et al. (2013) Proteomics guided discovery of flavopeptins: anti-proliferative aldehydes synthesized by a reductase domain-containing non-ribosomal peptide synthetase. J Am Chem Soc 135:10449-56
|Chen, Yunqiu; Unger, Michelle; Ntai, Ioanna et al. (2013) Gobichelin A and B: Mixed-Ligand Siderophores Discovered Using Proteomics. Medchemcomm 4:233-238
|Chen, Yunqiu; Ntai, Ioanna; Ju, Kou-San et al. (2012) A proteomic survey of nonribosomal peptide and polyketide biosynthesis in actinobacteria. J Proteome Res 11:85-94
|Vagstad, Anna L; Bumpus, Stefanie B; Belecki, Katherine et al. (2012) Interrogation of global active site occupancy of a fungal iterative polyketide synthase reveals strategies for maintaining biosynthetic fidelity. J Am Chem Soc 134:6865-77
|Ames, Brian D; Haynes, Stuart W; Gao, Xue et al. (2011) Complexity generation in fungal peptidyl alkaloid biosynthesis: oxidation of fumiquinazoline A to the heptacyclic hemiaminal fumiquinazoline C by the flavoenzyme Af12070 from Aspergillus fumigatus. Biochemistry 50:8756-69
|Evans, Bradley S; Ntai, Ioanna; Chen, Yunqiu et al. (2011) Proteomics-based discovery of koranimine, a cyclic imine natural product. J Am Chem Soc 133:7316-9
|Evans, Bradley S; Robinson, Sarah J; Kelleher, Neil L (2011) Surveys of non-ribosomal peptide and polyketide assembly lines in fungi and prospects for their analysis in vitro and in vivo. Fungal Genet Biol 48:49-61
|Hollenhorst, Marie A; Ntai, Ioanna; Badet, Bernard et al. (2011) A head-to-head comparison of eneamide and epoxyamide inhibitors of glucosamine-6-phosphate synthase from the dapdiamide biosynthetic pathway. Biochemistry 50:3859-61
Showing the most recent 10 out of 57 publications


text:
Corporations Are Not People and five other books are listed in this election-season roundup of "six great books about money in politics." I'm proud to be in such good company, and hope that you can check out all of them.
Twitter Updates
- RT @usapromise: Poll: 89% Of Illegal Immigrants Would Prefer Path To Corporate Status theonion.com/article/poll-8… via @theonion 9 hours ago
Corporations Are Not People
". . . that government of the people, by the people, and for the people shall not perish from the Earth."


text:
Antibody-mediated delivery of IL-10 inhibits the progression of established collagen-induced arthritis
DOI: 10.1186/ar2115
© Trachsel et al.; licensee BioMed Central Ltd. 2007
Received: 12 October 2006
Accepted: 29 January 2007
Published: 29 January 2007
The antibody-mediated targeted delivery of cytokines to sites of disease is a promising avenue for cancer therapy, but it is largely unexplored for the treatment of chronic inflammatory conditions. Using both radioactive and fluorescent techniques, the human monoclonal antibodies L19 and G11 (specific to two markers of angiogenesis that are virtually undetectable in normal adult tissues) were found to selectively localize at arthritic sites in the murine collagen-induced model of rheumatoid arthritis following intravenous (i.v.) administration. The same animal model was used to study the therapeutic action of the L19 antibody fused to the cytokines IL-2, tumour necrosis factor (TNF) and IL-10. Whereas L19–IL-2 and L19–TNF treatment led to increased arthritic scores and paw swellings, the fusion protein L19–IL-10 displayed a therapeutic activity, which was superior to the activity of IL-10 fused to an antibody of irrelevant specificity in the mouse. The anti-inflammatory cytokine IL-10 has been investigated for the treatment of patients with rheumatoid arthritis, but clinical development plans have been discontinued because of a lack of efficacy. Because the antigen recognised by L19 is strongly expressed at sites of arthritis in humans and identical in both mice and humans, it suggests that the fusion protein L19–IL-10 might help overcome some of the clinical limitations of IL-10 and provide a therapeutic benefit to patients with chronic inflammatory disorders, including arthritis.
The therapeutic potential of recombinant cytokines is often limited by severe toxicities, even at low doses, thus preventing dose escalation and the establishment of a sufficient concentration at target tissues. In principle, monoclonal antibodies could be used to deliver cytokines to sites of disease, thus increasing their potency and sparing normal tissues. Indeed, several antibody–cytokine fusion proteins have been investigated for cancer therapy applications, often with impressive therapeutic results [1–4].
Components of the modified extracellular matrix (ECM) are particularly attractive for antibody-based targeting applications, because these antigens are often stable and abundant [5]. In the past, our group has focused its efforts on the development of human monoclonal antibodies, which recognise ECM components that are virtually absent in normal tissues but strongly expressed at sites of disease and have a prominent perivascular pattern of expression [6]. Splice isoforms of abundant ECM components seem to be particularly suited for antibody-mediated in vivo targeting applications. For example, the extra domai [7–10] and C domain of tenascin-C [11–13] are two of the most promising markers of angiogenesis, which can be targeted using the high-affinity monoclonal antibodies L19 and G11, respectively [14–19]. Several derivatives of the L19 antibody have exhibited impressive therapeutic activity in animal models of cancer [1, 2, 4, 20–24] and angiogenesis-related ocular disorders [25]. In particular, the antibody–cytokine fusions L19–IL-2 and L19–tumour necrosis factor (TNF) and the radiolabelled antibody SIP(L19) (SIP, small immunoprotein) are currently in clinical development [26, 27].
The antibody-mediated targeted delivery of cytokines is largely unexplored in arthritis and other chronic inflammatory diseases. Although it is well established that pro-inflammatory cytokines (such as TNF and IL-1) can have a negative effect on arthritis [28, 29], anti-inflammatory cytokines might provide a therapeutic benefit. For example, IL-10 has been shown in a collagen-induced arthritis (CIA) mouse model to inhibit paw swelling and an increase in arthritic score [30–33]. Recombinant IL-10 was found to synergize with TNF-blocking antibodies [33] and has been tested in clinical trials in combination with methotrexate [34, 35]. The clinical development of recombinant human IL-10 was discontinued because of a lack of efficacy of the compound in humans.
In this article, we show that both L19 and G11 antibodies display an impressive ability to selectively localize at sites of arthritis in the CIA mouse model. Furthermore, whereas L19–IL-2 and L19–TNF treatment led to increased arthritic scores and paw swelling compared with controls, the fusion protein L19–IL-10 displayed therapeutic activity, which was superior to the activity of IL-10 fused to an antibody of irrelevant specificity in the mouse. These findings might be of clinical significance because EDB has an identical sequence in humans and mice [7, 36], is virtually absent in normal adult tissues [37] and is strongly expressed at arthritic sites in patients [38–40].
Materials and methods
Animal model
Male DBA/1 mice (8–12 weeks' old) were immunized by intradermal injection at the base of the tail with 200 μg of bovine type II collagen (MD Biosciences, Egg, Zurich, Switzerland) emulsified with equal volumes of Freund's complete adjuvant (MD Biosciences). The procedure was repeated 2 weeks after the first immunization, but incomplete Freund's adjuvant (MD Biosciences) was used to emulsify the collagen in the second procedure. Mice were inspected daily and each mouse that exhibited erythema and/or paw swelling in one or more limbs was assigned to an imaging or treatment study.
Arthritis was monitored using two disease indices (clinical score and paw swelling). For the clinical score, each limb was graded daily in a nonblinded fashion (0 = normal, 1 = swelling of one or more fingers of the same limb and 2 = swelling of the whole paw), with a maximum possible score of 8 per animal. Paw swelling was assessed every second day using a calliper to measure the thickness of each limb under isoflurane anaesthesia. The paw thickness of each animal was expressed as the mean value of all four paws.
Immunohistochemical analysis
Frozen sections of arthritic paws were fixed in ice-cold acetone and stained for EDB. For immunohistochemical analysis, the L19 antibody was used in the miniantibody format (SIP(L19)). As secondary detection antibodies, we used rabbit antihuman immunoglobulin (Ig) E (Dako, Golstrup, Denmark) followed by biotinylated antirabbit IgG (Biospa, Milan, Italy) and streptavidin–alkaline phosphatase complex (Biospa). Fast red TRsalt (Sigma, Saint Louis, Missouri, USA) was used to develop the staining. Slides were counterstained with haematoxylin, mounted with Glycergel® mounting medium (Dako) and analysed with a Zeiss Axiovert S100 TV microscope (Feldbach, Switzerland).
For immunofluorescence, a double staining for EDB and CD31 was performed. The following primary antibodies were used: SIP(L19) and rat antimouse CD31 (BD Pharmingen, Erembodegen, Belgium). As secondary detection antibodies, we used rabbit antihuman IgE (Dako), followed by Alexa Fluor 594 goat antirabbit IgG (Molecular Probes, Leiden, The Netherlands) for EDB and Alexa Fluor 488 goat antirat IgG (Molecular Probes) for CD31. Slides were mounted and analysed, as described above.
Near-infrared imaging of arthritic paws
The selective accumulation of SIP(L19) and SIP(G11) in arthritic mice was tested by near-infrared imaging analysis, as described by Birchler and colleagues [41]. Briefly, purified SIP(L19), SIP(G11) and SIP(HyHEL10) were labelled using Alexa750 (Molecular Probes), according to the manufacturer's recommendations, and injected into the tail vein of arthritic mice. Mice were anaesthetized using ketamine, 80 mg/kg body weight, and medetomidine, 0.2 mg/kg body weight, and imaged in a near-infrared mouse imager 24 hours after injection [41].
Biodistribution experiments
The in vivo targeting performance of SIP(L19) in arthritic mice was evaluated by biodistribution analysis, as described previously [14, 17]. Briefly, purified SIP(L19), SIP(G11) and SIP(F16) were radio-iodinated and injected into the tail vein of arthritic mice (5 μg corresponding to 12 μCi SIP(L19), 5 μg corresponding to 10 μCi SIP(G11) and 5 μg corresponding to 11 μCi SIP(F16) per mouse). Mice were sacrificed 24 hours after injection and their paws were exposed to a phosphorimager screen (Fujifilm, Dielsdorf, Switzerland) for 1 hour and read in a PhosphorImager (Fujifilm BAS-5000).
Cloning of L19–IL-10 and HyHEL10–IL-10
The human IL-10 gene was amplified from a commercial cDNA panel (Clontech, Basel, Switzerland) by PCR using the following primer sequences: a backward antisense primer, 5'-AGCCCAGGCCAGGGCA-CCCAGTCTG-3'; and a forward sense primer, 5'-GTTTCGTATCTTCATT-GTCATGTAGGCTTCTATG-TAGTTGATGAAGATG-3'. The resulting fragment was then further amplified using the following primer sequences: a backward antisense primer, 5'-TCGGGTAGTAGCTCTTCCGGCTCATCGTCCAGCGGCAGCCCAGGC-CAGGGCACC-3'; and a forward sense primer, 5'-TAATGGTGATGGTGATGGTGGTTTCGTATCTTCATT-GTCATGTAGGCTTC-3', which appended part of a 15 amino acid linker (SSSSG)3 at its N-terminal and a His6 tag, stop codon and NotI restriction site at its C-terminal.
The genes for the single-chain variable fragments (L19) and (HyHel10) were coamplified with a signal peptide using the following primer pairs, respectively: a backward antisense primer, 5'-CCCAAGCTTGTCGACCATGGGCTGGAGCC-3' and a forward sense primer, 5'-GAGCCGGAAGAGCTACTACCCGATGAGGAAGATTTGATTTCCACCTTGGTCCCTTG-3'; and a backward antisense primer, 5'-CCCAAGCTTGTCGACCATGGGCTGGAGCC-3' and a forward sense primer, 5'-GAGCCGGAA-GAGCTACTACCCGATGAGGAAGATTTTATTTCCAGCTTGGTCCCC-3'. Using this strategy, a HindIII restriction site was inserted at the N-terminal and a complementary part of the linker sequence was inserted at the C-terminal.
The single-chain Fv and IL-10 fragments were then assembled using PCR and cloned into the HindIII and NotI restriction sites of the mammalian cell-expression vector pcDNA3.1(+) (Invitrogen, Basel, Switzerland).
Expression and purification of L19–IL-10 and HyHEL10–IL-10
HEK293 cells were stably transfected with the previously described plasmids and selection was carried out in the presence of G418 (0.5 g/l). Clones of G418-resistant cells were screened for expression of the fusion protein by ELISA using a recombinant EDB of human fibronectin or lysozyme as antigens and an anti-His6-tagged antibody (Sigma) for detection. The fusion proteins were purified from the cell-culture medium by affinity chromatography over antigen columns, as described previously [17, 42]. The size of the fusion proteins was analysed in reducing and nonreducing conditions on SDS-PAGE and in native conditions by FPLC gel filtration on a Superdex S-200 exclusion column (Amersham Pharmacia Biotech, Dübendorf, Switzerland).
The in vivo targeting performance of L19–IL-10 in tumour-bearing mice was evaluated by biodistribution analysis, as described previously [14, 17]. Briefly, purified L19–IL-10 was radio-iodinated and injected into the tail vein of female 129Sv mice grafted with a subcutaneous F9 tumour (3 μg corresponding to 4 μCi L19–IL-10 per mouse). Mice were sacrificed 24 hours after injection; the tumours and organs were weighed and counted.
Biological activity of human IL-10 was determined by its ability to induce the IL-4-dependent proliferation of MC/9 cells [43] using a colourimetric thiazole blue (MTT) dye-reduction assay (Sigma). In a 96-well microtitre plate, 10,000 MC/9 (murine mast cell line) (ATCC-LCG, Molesheim Cedex, France) cells/well in 200 μl of medium containing 5 pg (0.05 units)/ml of murine IL-4 (eBiosciences, San Diego, CA, USA) were treated for 48 hours with varying amounts of human IL-10. The human IL-10 standard and fusion proteins were used at a maximum concentration of 100 ng/ml IL-10 equivalents and serially diluted. To this, 10 μl of 5 mg/ml MTT was added and the cells were incubated for 3–5 hours. The cells were then centrifuged, lysed with dimethylsulfoxide (DMSO) and read for absorbance at 570 nm.
Targeted delivery of cytokines to arthritic lesions
Immunocytokines L19–IL-2 and L19–TNF-α were prepared, as described previously [1, 2]. Each mouse that exhibited erythema and/or swelling of one or more paws was randomly assigned to a treatment or control group and therapy was started. Mice were given an i.v. injection in the lateral tail vein with saline or L19–IL-2, L19–TNF-α or L19–IL-10 diluted in a volume of 200 μl of saline. The cumulative doses for the fusion proteins were 60 μg of L19–IL-2, 15 μg of L19–TNF and 450 μg of L19–IL-10 per mouse. The arthritic score was evaluated daily in a nonblinded fashion. A scoring system was used with the following scale: 0 = normal, 1 = swelling of single fingers or toes and 2 = pronounced oedematous swelling of the whole paw [29]. Swelling of the paws was measured every second day using a calliper and the paw thickness of each animal was expressed as the mean score of all four paws. The results are displayed as the mean ± standard error of the mean (SEM) for each group; each group contained seven mice. Experiments were performed in agreement with Swiss regulations and under a project license granted by the Veterinäramt des Kantons Zürich, Switzerland (180/2004).
Comparison of targeted versus systemic application of IL-10
Each mouse that exhibited erythema and/or swelling of one or more paws was randomly assigned to a treatment or control group and therapy was started. Mice were given an i.v. injection in the lateral tail vein with saline, L19–IL-10 (3 × 150 μg) or HyHel-IL-10 (3 × 150 μg). Six mice were analysed per group. The arthritic score was evaluated daily in a nonblinded fashion and paw thickness was measured every second day. Experiments were performed in agreement with Swiss regulations and under a project license granted by the Veterinäramt des Kantons Zürich (180/2004).
Statistical analysis
Data are expressed as the mean ± SEM. Differences in the arthritis score between different populations of mice were compared using a Student t test. P < 0.05 was considered significant.
Histochemical analysis of arthritic paws
The human monoclonal antibodies L19 and G11 selectively accumulate at sites of arthritis
We studied the in vivo targeting performance of L19 and G11 in miniantibody format (SIP) [14] in the CIA mouse model using both fluorescence and radioactivity for antibody detection. The SIP format consists of a single-chain Fv antibody fragment linked to the CH4 domain of human IgE, giving rise to a homodimeric protein of 80 kDa.
Twenty-four hours after i.v. injection of SIP(L19) and SIP(G11), which were radiolabelled with iodine (125I), the mice were sacrificed and paws were imaged by autoradiography. A preferential accumulation of radioactivity was observed in the inflamed extremities of mice injected with SIP(L19) and SIP(G11), whereas no preferential antibody accumulation could be detected in mice exhibiting comparable grades of inflammation that had been injected with a SIP antibody of irrelevant specificity in the mouse. Biodistribution analysis performed with radiolabelled antibody preparations have previously shown that the percentage injected dose of antibodies in SIP format per gram of tissue typically lies below 1% 24 hours after injection, both for blood and for all major organs [14]. The ranges of lesion to nonaffected paw ratios measured by phosphorimaging were 3.3–8.5 for SIP(L19) and 2.0–4.9 for SIP(G11) (Figure 2).
Cloning, expression and characterization of single-chain Fv–human IL-10 fusion proteins
The human antibody fragments single-chain Fv(L19) and single-chain Fv(HyHEL10), preceded by a signal sequence required for secretion of recombinant proteins, were genetically fused to human IL-10 with a 15 amino acid linker between the C-terminal of the single-chain Fv fragment and the N-terminal of human IL-10. A His6 tag was appended to the C-terminal of the fusion proteins and the resulting antibody derivatives were expressed in stably transfected HEK293 cells. L19–IL-10 and HyHEL10–IL-10 were purified from the culture medium by affinity chromatography on antigen columns at yields of 1–2 mg/l. Both fusion proteins migrated at the expected size of 43 kDa in reducing and nonreducing conditions (Figure 3c). The size-exclusion chromatography profile of the purified fusion proteins showed a main peak eluting at 13.5 ml, corresponding to the biologically active heterodimer, and a lower peak eluting at 16 ml, corresponding to the monomer (Figure 3d). The in vivo targeting properties of a radio-iodinated preparation of L19–IL-10 were evaluated in a biodistribution experiment [17] in 129SvEv mice carrying subcutaneous F9 teratocarcinomas [4]. Favourable tumor : organ ratios (ranging from 7 : 1 to 128 : 1) were observed 24 hours after i.v. administration (Figure 3e). The biological activity of human IL-10 was confirmed, by the ability of the two fusion proteins to induce IL-4-dependent proliferation of MC/9 cells [43], using the colourimetric MTT dye-reduction assay (data not shown).
Effect of targeted delivery of cytokines to arthritic lesions
In a first-therapy experiment, we compared the potential of L19–IL-10 with L19–IL-2 and L19–TNF, using mice with CIA. Saline-injected mice were used as a control group. Mice received three injections every 48 hours, starting on day 1 after the onset of arthritis. The cumulative doses, which were equal to the doses previously used for tumour therapy experiments [1, 2], were 60 μg of L19–IL-2 and 15 μg of L19–TNF. Each mouse received a dose of 450 μg of L19–IL-10 in this experiment and subsequent experiments with antibody–IL-10 fusion proteins, in line with IL-10 doses previously found to be active and nontoxic in mice [45].
Comparison of targeted delivery compared with systemic application of IL-10
To demonstrate a therapeutic advantage of a targeted version of IL-10, compared with the untargeted cytokine, the two fusion proteins L19–IL-10 and HyHEL10–IL-10 were investigated in the CIA model. Similar to the previous experiment, arthritic mice were treated with three injections of L19–IL-10, HyHEL10–IL-10 or saline every second day, starting on the first day of arthritis onset. For both fusion proteins, the cumulative dose administered to each mouse was 450 μg. As expected, L19–IL-10 showed a significant therapeutic response compared with the control (saline) group (P = 0.037), with both the arthritic score and paw swelling remaining low until day 9 after arthritis onset (that is 4 days after the last injection). Consistent with previous observations of the therapeutic activity of IL-10 in this model, the nontargeted HyHEL10–IL-10 fusion protein displayed a therapeutic benefit compared with the saline control [33]; however, this was not as efficient as L19–IL-10 (Figure 4c,d).
The expression of fibronectin and tenascin-C isoforms at sites of arthritis opens new biomolecular avenues for the treatment of arthritic conditions. The L19 and G11 antibodies displayed the impressive ability to localize in affected limbs in a mouse model of CIA. By contrast, antibodies of irrelevant specificity in the mouse failed to accumulate (Figure 2). It is conceivable that the L19 and G11 antibodies could be used for the molecular imaging of arthritis in patients using positron emission tomography (PET) [46], near-infrared fluorescence [36, 47], ultrasound [48] or magnetic resonance imaging (MRI) methodologies [49, 50]. Fluorescence-based approaches might be particularly attractive for studying inflamed joints and planning immunophotodynamic therapy experiments using antibody-photosensitizer conjugates [21, 25].
The ability of L19 and G11 to target arthritis in vivo raises the question of whether optimal antibody functionalization strategies could be used for therapeutic purposes. In the mouse model, the performance of L19–IL-10 was superior to untargeted IL-10 and similarly potent as TNF-neutralizing antibodies [28, 33]. Previous reports of synergies between IL-10 and TNF-blocking agents and between IL-10 and methotrexate strongly suggest that L19–IL-10 should be clinically investigated (either alone or in combination) as a superior alternative to recombinant human IL-10. Clinical development of recombinant human IL-10 was discontinued because of a lack of efficacy in clinical trials, but there is a consensus that targeted delivery of this cytokine to sites of inflammation might lead to a dramatic enhancement of the therapeutic index [51, 52].
The antibody-mediated targeted delivery of pro-inflammatory cytokines (such as IL-2, IL-12, TNF and interferon (IFN) γ) has resulted in impressive increase in the therapeutic index of these biopharmaceuticals, with striking curative activities in animal models of cancer [3, 6, 26, 53]. In summary, the development of anti-inflammatory cytokine-antibody fusion proteins is a novel field of research, which promises to deliver novel biopharmaceutical agents of superior quality. Similar to cancer research, the identification and validation of pathology-associated targets is of fundamental importance to antibody development. Novel proteomic technologies derived from terminal perfusion of mouse models of pathology with biotinylation reagents, followed by a mass spectrometry-based comparative analysis, might be used in the future for the discovery of novel accessible markers of arthritis [54, 55].
The results obtained with the EDB of fibronectin and C domain of tenascin-C suggest that components of the modified ECM, generated by a mechanism of alternative splicing and displaying restricted patterns of expression in normal organs, might be ideal candidates for the development of antibody-based therapeutic strategies. These findings might be of clinical significance because the EDB has an identical sequence in humans and mice [7, 36], is virtually absent in normal adult tissues [37] and is strongly expressed at arthritic sites in patients [38–40].
collagen-induced arthritis
extracellular matrix
extra domain B of fibronectin
enzyme-linked immunosorbent assay
fast performance liquid chromatography
- Ig:
- i.v.:
magnetic resonance imaging
thiazole blue
polymerase chain reaction
- scFv:
single chain variable fragment
standard error of the mean
small immunoprotein
tumour necrosis factor.
Financial contributions from the Swiss National Science Foundation, ETH Zurich and Bundesamt für Bildung und Wissenschaft (EU Project STROMA) are gratefully acknowledged.
Authors' Affiliations
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This project received 16 bids from talented freelancers with an average bid price of $569 USD.Get free quotes for a project like this
Project Budget$250 - $750 USD
Total Bids16
Project Description
Looking to add a virtual tour, (similar to google street view) to a company website.
The company has a manufacturing facility, and would like to show it to customers around the world.
We're not looking to just 360 views, but also going back and forth, between different rooms/area
Preferably without the use of flash.
I also need advice on how to prepare the photos and videos that you will need to create it. The site is Wordpress and Ill provide the specs of the host for compatibility if needed.
The job will start around the beginning of July, tentatively.
Thanks and regards,
Skills Required
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ooty tourism
248 hotels in ooty
64 Restaurants in
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#1 of 248 Hotels in Ooty
Taj Savoy Hotel
77, Sylks Road, Udhagamandalam, Sylks Road,
Location. Savoy Hotel is located in Udhagamandalam, close to Government Museum, St. Stephen's Church, and Oooty Lake. Nearby points of interest also include Botanical Gardens. Resort Features. Savoy Hotel's restaurant serves breakfast, lunch, and dinner. A bar/lounge is open for drinks. Room service is available 24 hours a day. The resort serves buffet breakfasts each morning in the coffee shop (surcharges apply). Recreational amenities include a children's club. This 3.5-star property has a 24-hour business center and offers a meeting/conference room and audio-visual equipment. Wireless and wired high-speed Internet access is available in public areas (surcharges apply) and the resort has an Internet point. This Udhagamandalam property has 70 square meters of event space consisting of a conference center, banquet facilities, and exhibit space. Valet parking and self parking are complimentary. Additional property amenities include a library, a coffee shop/café, and a concierge desk. This is a smoke-free property. Guestrooms. 40 air-conditioned guestrooms at Savoy Hotel feature fireplaces and minibars. Rooms are all accessible via exterior corridors. Accommodations offer garden views. These individually furnished and decorated rooms include desks and ergonomic chairs. Bathrooms feature bathtubs or showers with handheld showerheads. They also offer phones, slippers, and scales. Wired high-speed and wireless Internet access is available for a surcharge. In addition to complimentary newspapers and laptop-compatible safes, guestrooms offer cordless phones with voice mail. 29-inch flat-panel televisions have premium satellite channels, free movie channels, and TV Internet access (surcharge). Also included are coffee/tea makers and complimentary bottled water. Guests may request irons/ironing boards, extra towels/bedding, and wake-up calls. A nightly turndown service is offered and housekeeping is available daily. Guestrooms are all non-smoking.
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#2 of 248 Hotels in Ooty
Hotel Kluney Manor
196/197, Patna House, Mount Pleasant, The Nilgiris, Behind Brinda Hospital, the nilgiris ooty, mount peasant road, ooty,
Located just 1.5 kilometres from the bus stand and railway station, Kluney Manor lies on the higher reaches of the town. It offers a splendid view of the race course and the surrounding countryside. It is just 2 kilometres away from the Lake and Botanical Garden.A 165 year old heritage property, Kluney Manor is reminiscent of the Raj era with a stately colonial building that sits pretty on a 2 acre landscaped area. Having been an ancestral home for generations, it was extensively renovated two years ago. Although it does retain its old world charm, the renovation was carried out to ensure that guests enjoy a comfortable stay with all modern luxuries that they are accustomed to.Facilities and ServicesCurrency exchange | Outdoor activities | Internet facility | Restaurant | Car rental | Laundry | Conference facilities| Coffee shop | Railway/Airport transfer | SightseeingRoom AmenitiesStudy table | Sofa | Living room | Television | Intercom | Shower | Hot/cold water | Bathroom toiletriesDiningKluney Manor's multi cuisine restaurant, Le Cluney Hall serves delectable Indian, Chinese and continental cuisines. Conferences & BanquetsWhile there is no formal conference hall, the hotel's lobby can be used for smaller gatherings. The banquet lawn in front of the building is ideal for hosting events, functions and parties for upto 200 guests. The hotel also takes care of the decorations and catering for parties.Internet: Available | Parking Facility: Not AvailableCheck-In Time: 12:00 Noon | Check Out Time: 12:00 Noon "
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#3 of 248 Hotels in Ooty
Reflections Guest House
1/B, Near North Lake Road, Near North Lake Road, north lake road, ooty,
LocationReflections Guest House is situated on North Lake Road in Ooty (Ootacamund), Tamil Nadu. The nearest airport from the guest house is Coimbatore Airport, around 100 km away, while Mettupalayam Railway Station (50 km) is the nearest major railhead. The bus stand is located closeby. Away from bustling cities, Reflections Guest House is a quaint budget property, catering to the travellers visiting the picturesque hill station of Ooty. The guest house overlooking the lake along with a comfortable stay provides easy access to tourist spots in and around Ooty. RoomsReflections Guest House has elegantly appointed guests rooms that are available in two categories, namely Semi Deluxe rooms and Standard Air-Conditioned rooms. All rooms are nicely furnished and equipped with essential facilities. In-room amenities available at Reflections Guest House include television with cable connection, and an attached washroom with hot and cold running water facility.Dining and FacilitiesBesides traditional delicacies of South India, the vegetarian restaurant at the guest house serves Indian, Chinese and Continental cuisines.Other facilities and services available at the guest house include a common living/dining room, travel desk, doctor on call, fireplace, laundry service and car rental service.
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#4 of 248 Hotels in Ooty
Destiny Farmstay
Vakkiel Thotum, Muligur Village, Emerald, Udagamandalam, Muligur Village,
Tucked away in a pristine, untouched valley of Avalanchi (about 25 km past Ooty), and set in the Niligiri Biosphere, Destiny gives you the real experience of a farm stay, blended with a wildlife adventure resort unlike any other. Its a holiday where every moment is anything but usual. Do so much, or do absolutely nothing but take in the sight of the lake, the hills and the clouds beneath you. With all the comforts of the good life, no matter how old you are or what you do, theres always a new experience to inspire & refresh you.
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#5 of 248 Hotels in Ooty
Green Park Resorts
Singara Road, Masinagudi, Near Private Bus Stand, Near Private Bus Stand, masinagudi, ooty,
Well-maintained rooms and first-rate amenities ensure a cosy stay. Overseeing city lights, the hotel offers comfort at its best.
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#6 of 248 Hotels in Ooty
Hotel Meadows Residency
Charring Cross, Coonoor Main Road, Near Hotel Sunpark Grand, Near Hotel Sunpark Grand, charring cross, ooty, coonoor main road, ooty,
Location. Hotel Meadows Residency is located in Udhagamandalam, close to Botanical Gardens, St. Stephen's Church, and Government Museum. Nearby points of interest also include Oooty Lake. Hotel Features. Hotel Meadows Residency features a restaurant. The hotel serves a complimentary continental breakfast. Complimentary wireless Internet access is available in public areas. Guest parking is complimentary. Additional property amenities include laundry facilities. Guestrooms. All guestrooms at Hotel Meadows Residency feature complimentary bottled water. Wireless Internet access is complimentary.
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#7 of 248 Hotels in Ooty
Gem Park Ooty
Sheddon Road, sheddon road, ooty,
Gem Park Ooty is located in Ooty overlooking the Doddabetta Valley, about 3 hours from Coimbatore airport and 4 hours from Mysore. The Ooty railway station and bus stop is just 10 minutes away from the hotel. Mettupalayam is 2 hours away. The local market is 2 km away, while the Ooty golf course is 5 km away from the hotel.The ornate fountain in the driveway of the Holiday Inn Gem Park prepares you for the grandeur of the hotel. On entering the lobby and reception area, you are welcomed in to a huge space bedecked with wooden carvings, paintings, chandeliers, seating areas and of course the staff.Facilities and ServicesSecretarial service | Housekeeping | Dining hall | Conference facilities | Coffee shop | Shopping arcade | Currency exchange | Internet facility | Laundry | Doctor on call | Safety deposit locker | Parking facility | Travel desk | Accepts major credit cardRecreational: Spa | Steam room | Jacuzzi | Sightseeing | Outdoor activities | Kid swimming pool | Golf | Recreational centre | Sauna | Gymnasium | Childrens play areaRoom AmenitiesSatellite TV | Telephone | Room service | AC | Sofa | Bathroom toiletriesDiningThe hotel boasts of the Toda Cafe, which serves a wide range of mouth watering Indian delicacies. The Jade Garden is a Chinese specialty restaurant overlooking the Dodabetta Peak. You can also unwind with a drink or two at the Valley Bar which offers from well-stocked Indian and imported liquors. You have a memorable time here with the pianist rendering wonderful music. Internet: Available | Parking Facility: AvailableCheck-In Time: 02:00 PM | Check Out Time: 12:00 PM "
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#8 of 248 Hotels in Ooty
The King's Cliff
Havelock Road, Strawberry Hill, Near Monarch Hotel Signboard, Near Monarch Hotel Signboard, havelock road, ooty,
The hotel is located away from the hustle and bustle of the main market area, providing peaceful solitude and yet it offers easy accessibility to all areas of tourist interest. Within a four kilometre radius lie the Lake, bus stand, railway station and the Botanical Garden.King's Cliff is a 110 year old heritage property that was converted into a hotel just 4 years ago. It has been sufficiently renovated to provide the most modern facilities to guests and yet it retains the old world charm and heritage associated with it. Facilities and ServicesCar rental | Laundry | Conference facilities| Travel desk | Parking facility | RestauarntRoom AmenitiesGuest slippers | Sofa | Fireplace | Living room | Television | Study table | Bathroom toiletriesDiningEarl's Secret, the multi cuisine restaurant serves delectable Indian, Chinese and Continental cuisines. A buffet spread is served for breakfast from 8:30 am to 10:30 am while lunch (12 noon to 3 pm) and dinner (7 pm to 10:30 pm) are offered through an a la carte menu. Internet: Not Available | Parking Facility: AvailableCheck-In Time: 11:00 AM | Check Out Time: 10:00 AM "
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#9 of 248 Hotels in Ooty
Hotel Welbeck Residency
Welbeck Circle, Club Road, The Nilgris, Club Road, the nilgiris ooty,
Hotel Welbeck Residency is situated at Welbeck Circle on Club Road in Ooty (Ootacamund). The nearest airport is Coimbatore Airport, approximately 85 km away, while Mettupalayam Railway Station is 40 km away. Ooty Railway Station is 2.5 km away from the hotel, and the bus stand is located closeby. Hotel Welbeck Residency is a luxury hotel, offering a comfortable stay to its guests along with warm hospitality. Set at a quaint location in Ooty, Hotel Welbeck Residency is known for its exquisite interiors, state-of-the-art amenities and world class service.Facilities and ServicesInternet facility | Conference facility | Doctor on call | Restaurant | Parking facility | Travel deskRoom AmenitiesHotel Welbeck Residency offers a wonderful choice of well-appointed guest rooms, which are categorised as Deluxe rooms, Super Deluxe rooms and Luxury rooms. Each room at Hotel Welbeck Residency has been beautifully designed and furnished in traditional Ooty theme with modern facilities. Guests have access to in-room amenities like television, telephone facility, daily newspaper, and an attached washroom with hot and cold water supply. For the convenience of guests, round the clock room service is also available. DiningHotel Welbeck Residency has a well-appointed multi-cuisine restaurant, where guests can savour delicacies of South India in addition to North Indian, Chinese and Continental dishes. Besides this, the hotel also has a well-stocked bar, which offers a fine selection of heady drinks including cocktails and mocktails. Internet: Available | Parking Facility: AvailableCheck-In Time: 12:00 PM | Check-Out Time: 12:00 PM"
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#11 of 248 Hotels in Ooty
British Cliff Club Resort
Up Hill of Race Horse, Mount Peasant Road, Near Blue Mountain School, Near Blue Mountain School, mount peasant road, ooty,
Located on Mount Peasant Road, Hotel British Cliff Club lies in the heart of Ooty. It is just 2 kilometers away from the Botanical Gardens and 33 kilometers away from Dodapetta Peak. Coonoor is just an hour drive away from the property.Hotel FacilitiesLawns/Garden, Outdoor activities, Activity center, Car rental, Laundry, Library, Doctor on call, Restaurant, Travel desk, DiscothequeRoom AmenitiesTelevision, Telephone, Room service, Mineral water, Hot/cold water, Internet access, Fruit basketDiningDining outlets at Hotel British Cliff Club include a multi cuisine indoor restaurant and a barbeque shack. The hotel also boasts of a coffee shop and a discotheque. For the convenience of guests, room service is also available.Internet: Available & Parking Facility: Not AvailableCheck-In Time: 12:00 PM & Check-Out Time: 12:00 PM"
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#12 of 248 Hotels in Ooty
Highland Hotel
4/278, Kotagiri Road, Doddabetta Junction, Near Doddabetta Road, The Nilgiris, Near Doddabetta Road, the nilgiris ooty, doddabette road, ooty, kotagiri, ooty,
Highland Hotel is located at Doddabetta Junction on Kotagiri Road in Ooty. It enjoys a convenient accessibility of 102 kilometres from Coimbatore Airport and 6 kilometres from railway station. Highland Hotel stands tall with all elegance and style. A contemporary structure with a traditional feel, Highland Hotel Ooty is the highest hotel in Southern India and an inspiring destination for leisure. Honeymooners & corporate travelers with tradition of uncompromising service and state of the art amenities, one can be sure that banquet, meeting or corporate retreat will be a sure success.Hotel FacilitiesBusiness Centre, Conference Hall, Executive Lounge, Internet Facility, Wi-Fi Enabled, Indoor Restaurant, Coffee Shop, Dining Hall, Babysitting Service, Doctor on Call, Laundry Service, Bicycles on Hire, Central Heater, Parking Facility, Travel Desk, Sightseeing, Shuttle Service, Taxi Services, BonfireRecreational ActivitiesLibrary, Activity Centre, Games Room, Outdoor Activities Chargeable FacilitiesAirport Transfer, Railway Station Transfer, Guide ServiceRoom AmenitiesAir-Conditioning (Heater), Living Room (only in suite rooms), Cable/Satellite Television, Intercom Facility, DVD Player (on request), Piped/Channel Music, Data Port, Direct Dial Telephone, Wi-Fi Internet Access, Mini Bar, Tea/Coffee Maker, Refrigerator, Mineral Water, Iron & Ironing Board (on request), Dining Table, Sofa, Study Table, Safe Deposit Locker, Electronic Safe, Daily Newspaper, Room Heater, Fireplace, Hot/Cold Water, Bath Tub, Bathroom Toiletries. Highland Hotel offers 53 well-appointed rooms and are categorized as Deluxe and Suite with efficient room service and house-keeping. DiningHighland Hotel offers a wide array of delectable cuisines in its all day multi-cuisine restaurant "Seasons" and "Highland Cafe". Internet: Available (Wi-Fi Enabled) & Parking Facility: Available Check-In Time: 12:00 PM & Check-Out Time:...
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#13 of 248 Hotels in Ooty
Green County Resort
Mistair Meadows, Coonoor Road, Lovedale Junction, Lovedale Junction, coonoor main road, ooty, lovedale, ooty,
A cosy stay is ensured by orderly rooms and excellent amenities. The hotel overlooks city lights and offers comfort at its best.
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#14 of 248 Hotels in Ooty
Fortune Resort Sullivan Court
123, Selbourne Road, Opposite Rose Garden, Opposite Rose Garden,
This 4 Star, leisure hotel is set on the gentle slopes at Selbourne Road. Amid lush green lawns and overlooking the Nilgiri Mountains are 67 comfortable rooms that are maintained with 24 hours room service, which includes complimentary breakfast to two adults. Guests can lounge at Pavilion - a 24 hours coffee shop-cum-multi-cuisine restaurant, and for some North-West Frontier cuisine one can visit Khyber Restaurant with an outdoor ambiance.
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#15 of 248 Hotels in Ooty
Glyngarth Villa Resorts
Golf Club Road, Finger Post, Ootacamund, Finger Post,
Built in 1850 - this colonial-style budget resort is sprawled across four acres of woods and greenery. There are three deluxe double bedrooms and two master deluxe bedrooms to choose from. Guests are offered Continental, Chinese and a variety of local cuisine from the kitchen of this hotel, which is prepared from garden fresh vegetables and garnished with fragrant spices. There are loads of recreational activities that are organized on request, namely jungle safaris and trekking.
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#16 of 248 Hotels in Ooty
Ponmari Residency
No. 26-D, Kotagiri Road, Near Botanical Garden, Charring Cross, Near Botanical Garden, botanical garden, ooty, charring cross, ooty, kotagiri, ooty,
Close to the Botanical gardens, this 3Star smoke-free property maintains 36 well-furnished rooms, which include 2 Royal Suites, 5 Royal Honey Suites and 5 Family rooms. Designed with wooden carvings, the hotel is maintained with Ponmari, a Multi-Cuisine Restaurant, which serves delicious and mouth-watering fare. Corporate travelers can make use of the Maris Conference Hall to conduct official events.
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