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What does Puck's soliloquy mean, at the end of A Midsummer Night's Dream?
2 Answers | Add Yours
I guess you can paraphrase Puck's final soliloquy that ends the play as follows: If the actors and events that you have seen have upset you, I recommend that you imagine it was all a dream and that you were asleep when you saw all of it. Audience, please don't get upset with me. If you pardon us, we will make everything OK. I am an honest kind of guy, and I promise that if you don't boo us, we will make it up. If not then you can call me a liar. So farewell to you all. Please clap your hands if we are friends and I will make it all OK. One of the points that you must realise in this soliloquy is that it touches on a major theme of the play - that of dreams vs reality. In Act V scene 1 we see the lovers debating about whether what happened to them was a dream or real, and here Puck echoes those thoughts to end the play on a very whimsical note. If we are offended by what happened, much better to pretend it was all a dream.
Puck is apologizing for the upsetting events in the play. Basically, he suggests to the audience that if the fairies' activities upset them, they could pretend it was all a dream and that the audience had merely slept there. Puck's final monologue sets things right with the audience.
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text:
You can't even remove anything from your library without making the files hidden or removing them from the directory they're in.
I haven't seen any screenshots of it where it's set up in the way I want it to be either. But I'm not even going to bother with that when I can't even manage which individual files I want to appear in my library, that's a dealbreaker for me.
Why would you want songs in your library to not be displayed in your library? Isn't that what creating playlists is for? I don't get it.
You can easily arrange music in your windows music section to appear in foobar in all sorts of ways.
Edited by Mani ATH 87 - 1/21/13 at 11:19am


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Our goal is to define how a bacterial cell integrates the timing of DNA replication and the polar positioning of the chromosomal origin region to control the temporal and spatial expression of cell cycle events. A lynch-pin in the temporal coordination of replication initiation and the transcription of genes encoding global cell cycle regulators is DnaA. This protein functions both to initiate replication and to activate the transcription of over 40 cell cycle-controlled genes. DnaA is controlled at multiple levels, including transcriptional regulation by differential methylation of its promoter that is linked to the progression of the replication fork, and the control of its activity by the replisome-associated HdaA protein. We will determine if changes in DNA methylation state that functions to 'clock'the sequential transcription of both dnaA and ctrA controls the temporal transcription of multiple cell cycle regulated genes, including those controlled by GcrA master regulator. We will also determine if a temporally-controlled dnaA anti- sense transcript contributes to the control of dnaA expression. The dynamic spatial deployment of proteins and the origin region of the chromosome are critical factors in cell cycle control. We have shown that upon replication of the chromosomal origin, the actual DNA sequence that moves toward, and is captured by, the cell pole is parS bound to the ParB partition protein. If the ParB/parS complex is not anchored to the pole, segregation of the rest of the chromosome is impaired and the FtsZ division ring is misplaced. We have recently identified PopZ as a protein that forms a polar polymeric network and functions to anchor ParB/parS to the new cell pole. Important questions are how the polar ribosome free zone formed by the PopZ network is established and how it functions. To define how ParB/parS moves across the cell to be captured by PopZ, we have initiated an analysis of the essential ParA segregation protein. We have generated multiple mutants of ParA and visualized ParA behavior both in vivo and in vitro. We will now identify and characterize the factors that mediate its role in DNA movement and determine the mechanism that drives chromosome segregation.
Based on our elucidation of the genetic circuitry that runs a bacterial cell cycle, we designed a new class of boron-based antibiotics that are in phase two trials. In addition, we identified a small molecule inhibitor of the MreB bacterial actin, with the goal of using this as the basis for a new family of antibiotics.
|Mera, Paola E; Kalogeraki, Virginia S; Shapiro, Lucy (2014) Replication initiator DnaA binds at the Caulobacter centromere and enables chromosome segregation. Proc Natl Acad Sci U S A 111:16100-5
|Gonzalez, Diego; Kozdon, Jennifer B; McAdams, Harley H et al. (2014) The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach. Nucleic Acids Res 42:3720-35
|Childers, W Seth; Xu, Qingping; Mann, Thomas H et al. (2014) Cell fate regulation governed by a repurposed bacterial histidine kinase. PLoS Biol 12:e1001979
|Ptacin, Jerod L; Gahlmann, Andreas; Bowman, Grant R et al. (2014) Bacterial scaffold directs pole-specific centromere segregation. Proc Natl Acad Sci U S A 111:E2046-55
|Schrader, Jared M; Zhou, Bo; Li, Gene-Wei et al. (2014) The coding and noncoding architecture of the Caulobacter crescentus genome. PLoS Genet 10:e1004463
|Hong, Sun-Hae; Toro, Esteban; Mortensen, Kim I et al. (2013) Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement. Proc Natl Acad Sci U S A 110:1674-9
|Ptacin, Jerod L; Shapiro, Lucy (2013) Chromosome architecture is a key element of bacterial cellular organization. Cell Microbiol 15:45-52
|Blair, Jimmy A; Xu, Qingping; Childers, W Seth et al. (2013) Branched signal wiring of an essential bacterial cell-cycle phosphotransfer protein. Structure 21:1590-601
|Christen, Beat; Abeliuk, Eduardo; Collier, John M et al. (2011) The essential genome of a bacterium. Mol Syst Biol 7:528
|McAdams, Harley H; Shapiro, Lucy (2011) The architecture and conservation pattern of whole-cell control circuitry. J Mol Biol 409:28-35
Showing the most recent 10 out of 25 publications


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Need Press ReleaseWriter
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Project Budget$30 - $250 USD
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Career Center
| The Administrator is responsible for the oversight and operations of the community. This effort includes the supervision, direction, and coordination of all community operations as well as ensuring compliance with all regulatory requirements. The Administrator establishes specific and measurable goals that align with the community's strategic plan, organizational growth and culture and ensures that resident satisfaction is maintained.
1. Models the transforming culture of Integrace by demonstrating knowledge and awareness of the mission, vision, and values through leadership practices and decisions. Consistently reveals Integrace's guiding principles and supporting behaviors of the transforming culture to all associates, visitors, and residents.
2. Provides oversight responsibility for all levels of leadership, culture philosophy and the values of Integrace into the specific functional discipline of the incumbent and executes assignments within a culture that is shared and collaborative across all Integrace communities.
3. Maintains a philanthropic leadership role in cultivating and developing major and planned charitable gifts that support Integrace and effectively represents Integrace to the local community at large.
4. Oversees the daily operations (all functions) of the facility to include, but not limited to: personnel management, resident care and satisfaction, financial considerations and budgets, and regulatory & legal compliance.
5. Creates a vision for success and builds relationships, internally and externally, to ensure vision attainment.
6. Partners with Executive and Senior Leadership to develop the strategic direction and long term initiatives for the community and facilitates successful strategic alignment in community operations.
7. Creates and maintains a positive atmosphere of leadership including continuous quality improvement, teamwork, respect, professionalism and accountability.
8. Performs other duties as assigned.
nursing home administrator, CCRC, NHA, administrator
|Master's Degree preferred, Bachelor's Degree Required in Healthcare Administration or related field. Nursing Home Administrator license required. 1 - 3 years direct experience working with the Dementia population required. At least 2 years as a manager or administrator in an assisted living or CCRC.


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We are all ambassadors for our island and the style of our hotel. When I meet with the chambermaids every morning – we have one per suite – I always ask them the same question Count Manfredi asked me twenty-five years ago: "What do our guests want? What else can we do to make the beauty of Punta Tragara unforgettable?" Just as it did then, the answer lies in the question, in always listening and giving guests our generosity and discretion.
By Anna Lardaro - Housekeeper


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Most Popular
Most Discussed
© 2016 MNM Media LLC.. Privacy Policy is applicable to you. All rights reserved.
Tag - DVD burner
Samsung shows Smart Media Hub with integrated DVD burner
Samsung has finally demonstrated its SE-208BW Smart Media Hub, which was originally announced in September. The device offers the same basic functionality as many other media hubs, however it adds a CD/DVD burner. Users can stream audio CDs or DVD content over Wi-Fi networks to play on smartphones, tablets or PCs.
iSkysoft DVD Creator 2.0 improves video importing, editing
iSkysoft Studio has upgraded its DVD burning software, DVD Creator. Version 2.0 adds support for additional output options including DVD Disc and DVD Folder, which can be used with most popular video formats. Other features include animated menu templates for creating DVD menus, along with the ability to save imported files to the Project File for later editing or re-burning.
Now AAPL Stock: 136.66 ( + 0.13 )
Cirrus creates Lightning-headphone dev kit
Apple supplier Cirrus Logic has introduced a MFi-compliant new development kit for companies interested in using Cirrus' chips to create Lightning-based headphones, which -- regardless of whether rumors about Apple dropping the analog headphone jack in its iPhone this fall -- can offer advantages to music-loving iOS device users. The kit mentions some of the advantages of an all-digital headset or headphone connector, including higher-bitrate support, a more customizable experience, and support for power and data transfer into headphone hardware. Several companies already make Lightning headphones, and Apple has supported the concept since June 2014. http://bit.ly/29giiZj
Apple Store app offers Procreate Pocket
The Apple Store app for iPhone, which periodically rewards users with free app gifts, is now offering the iPhone "Pocket" version of drawing app Procreate for those who have the free Apple Store app until July 28. Users who have redeemed the offer by navigating to the "Stores" tab of the app and swiping past the "iPhone Upgrade Program" banner to the "Procreate" banner have noted that only the limited Pocket (iPhone) version of the app is available free, even if the Apple Store app is installed and the offer redeemed on an iPad. The Pocket version currently sells for $3 on the iOS App Store. [32.4MB]
Porsche adds CarPlay to 2017 Panamera
Porsche has added a fifth model of vehicle to its CarPlay-supported lineup, announcing that the 2017 Panamera -- which will arrive in the US in January -- will include Apple's infotainment technology, and be seen on a giant 12.3-inch touchscreen as part of an all-new Porsche Communication Management system. The luxury sedan starts at $99,900 for the 4S model, and scales up to the Panamera Turbo, which sells for $146,900. Other vehicles that currently support CarPlay include the 2016 911 and the 2017 models of Macan, 718 Boxster, and 718 Cayman. The company did not mention support for Google's corresponding Android Auto in its announcement. http://bit.ly/295ZQ94
Apple employees testing wheelchair features
New features included in the forthcoming watchOS 3 are being tested by Apple retail store employees, including a new activity-tracking feature that has been designed with wheelchair users in mind. The move is slightly unusual in that, while retail employees have previously been used to test pre-release versions of OS X and iOS, this marks the first time they've been included in the otherwise developer-only watchOS betas. The company is said to have gone to great lengths to modify the activity tracker for wheelchair users, including changing the "time to stand" notification to "time to roll" and including two wheelchair-centric workout apps. http://bit.ly/2955JDa
SanDisk reveals two 256GB microSDXC cards
SanDisk has introduced two 256GB microSDXC cards. Arriving in August for $150, the Ultra microSDXC UHS-I Premium Edition card offers transfer speeds of up to 95MB/s for reading data. The Extreme microSDXC UHS-I card can read at a fast 100MB/s and write at up to 90MB/s, and will be shipping sometime in the fourth quarter for $200. http://bit.ly/294Q1If
Apple's third-quarter results due July 26
Apple has advised it will be issuing its third-quarter results on July 26, with a conference call to answer investor and analyst queries about the earnings set to take place later that day. The stream of the call will go live at 2pm PT (5pm ET) via Apple's investor site, with the results themselves expected to be released roughly 30 minutes before the call commences. Apple's guidance for the quarter put revenue at between $41 billion and $43 billion. http://apple.co/1oi1Pbm
Twitter stickers slowly roll out to users
Twitter has introduced "stickers," allowing users to add extra graphical elements to their photos before uploading them to the micro-blogging service. A library of hundreds of accessories, props, and emoji will be available to use as stickers, which can be resized, rotated, and placed anywhere on the photograph. Images with stickers will also become searchable with viewers able to select a sticker to see how others use the same graphic in their own posts. Twitter advises stickers will be rolling out to users over the next few weeks, and will work on both the mobile apps and through the browser. http://bit.ly/29bbwUE


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Russell on Religion
Selections from the Writings of Bertrand Russell
By (author) Bertrand Russell
Edited by Louis Greenspan Stefan Andersson Escher
Format: Paperback
Normal Price: $58.99
Your Price: $53.09 AUD, inc. GST
Shipping: $7.95 per order
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Availability: Available, ships in 7-10 days
Russell on Religion by Bertrand Russell
Book DescriptionRussell on Religion presents a comprehensive and accessible selection of Bertrand Russell's writing on religion and related topics from the turn of the century to the end of his life. The influence of religion pervades almost all Bertrand Russell's writings from his mathematical treatises to his early fiction. Russell contends with religion as a philosopher, as a historian, as a social critic and as a private individual. The papers in this volume are arranged chronologically for optimum coherence of the development of Russell's thinking and are divided into five main sections: * Personal statements * Religion and Philosophy * Religion and Science * Religion and Morality * Religion and History. Students at all levels will find this a valuable insight into Russell's thought on religion.
Buy Russell on Religion book by Bertrand Russell from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780415180924
(216mm x 138mm x 20mm)
Pages: 272
Imprint: Routledge
Publisher: Taylor & Francis Ltd
Publish Date: 9-Sep-1999
Country of Publication: United Kingdom
Other Editions...
Books By Author Bertrand Russell
Philosophy and Politics, Paperback (June 2016)
This book presents the 1946 National Book League lecture, delivered by Bertrand Russell on the relationship between philosophies and the development of political systems.
Impact of Science on Society, Paperback (April 2016)
Previously published: New York: Simon and Schuster, 1953.
In Praise of Idleness, Paperback (March 2016)View all books by Bertrand Russell
New York Times bestselling author Bradley Trevor Greive breathes new life into Bertrand Russell's classic work, In Praise of Idleness, with a magical package that includes BTG's new introduction, biographical afterword, historical notes, additional quotations and...
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Automotive Locksmith
Give Hemet Locksmith a call when you need the help of an automotive locksmith. We act quickly by sending out locksmith technicians your way in a hurry
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Is Katie Holmes pregnant?
Are Katie and Tom expecting another baby?
Katie Holmes is expecting her second child with Tom Cruise.
The couple joined Victoria Beckham as guests of honour at David's last football match at Real Madrid. The four of them then joined the post-match party.
Suri was left in Berlin where Tom has been preparing for a film.
A source said: "Katie and Tom are thrilled about having another baby on the way. She is glowing with happiness."
Is Katie copying Posh with her new chic bob? Have your say on the Cosmo Gossip forum.
Want hair as sleek as Katie's? Check out our hair masterclass.


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- Product 708090
BBE Sonic Maximizer Plug-In
- Write a Review
The BBE Sonic Maximizer Plug-In is a new software version of BBE's award-winning Sonic Maximizer that features revised code to support more platforms...Click To Read More About This Product
We're sorry-this item is unavailable.
Find a similar product below or contact our experts for a recommendation of great alternatives. Call us at 800-449-9128.Customers Also Viewed:
Based on the 4th generation 882i analog unit.
The BBE Sonic Maximizer Plug-In is a new software version of BBE's award-winning Sonic Maximizer that features revised code to support more platforms including Mac, PC VST, and Direct X. The Sonic Maximizer Plug-In allows unlimited user-definable presets, realtime preset changes, and full MIDI implementation. It's like having a rack full of the 882i units at your command for processing multiple tracks. The
Sonic Maximzeer Plug-In can add brilliance, depth, detail, and definition to your music in many PC audio software applications that accept Direct X plug-ins, from multi-track recording to sampling to remixing.
The BBE Process--"What it Is"
Loudspeakers have difficulty working with the electronic signals supplied by an amplifier. These difficulties cause such major phase and amplitude distortion that the sound reproduced by the speaker differs significantly from the sound produced by the original source.
In the past, these problems proved unsolvable and were thus relegated to a position of secondary importance in audio system design. However, phase and amplitude integrity is essential to accurate sound reproduction. Research shows that the information which the listener translates into the recognizable characteristics of a live performance are intimately tied into complex time and amplitude relationships between the fundamental and harmonic components of a given musical note or sound. These relationships define a sound's "sound".
When these complex relationships pass through a speaker, the proper order is lost. The higher frequencies are delayed. A lower frequency may reach the listener's ear first or perhaps simultaneously with that of a higher frequency. In some cases, the fundamental components may be so time-shifted that they reach the listener's ear ahead of some or all of the harmonic components.
This change in the phase and amplitude relationship on the harmonic and fundamental frequencies is technically called "envelope distortion." The listener perceives this loss of sound integrity in the reproduced sound as "muddy" and "smeared." In the extreme, it can become difficult to tell the difference between musical instruments, for example, an oboe and a clarinet.
BBE Sound, Inc. conducted extensive studies of numerous speaker systems over a ten year period. With this knowledge, it became possible to identify the characteristics of an ideal speaker and to distill the corrections necessary to return the fundamental and harmonic frequency structures to their correct order. While there are differences among various speaker designs in the magnitude of their correction, the overall pattern of correction needed is remarkably consistent.
The BBE Process is so unique that 42 patents have been awarded by the U.S. Patent Office.
Order today and make your projects sound clearer, brighter, warmer, and punchier.
Reviewed by 9 customers
I have used this plug-in with Sound Forge for years to add punch to voice-overs for broadcast. One bonus is it's easy to use. We are a Mac based video production company, and the only negative is it doesn't support AU plug-ins. But, we have kept a PC running Sound Forge, mainly so we can continue to use this specific filter.
I bought this plugin a year or two ago and never blinked once in regret. It's great for making the bass richer and increasing the clarity of mids and highs. You can overdo it so avoid extreme settings. On sale now ? That's a good deal. I use the low end hardware model too, for enhancing analog mix playback.
This little plug really works, so well that it's hard to imagine mastering without it. I've had it for a while, and much later, got Ozone 3. With all of Ozone's separate features, it got easy to forget about the BBE for a while. As time went on, the Sonic Maximizer has regained a place in my standard mastering procedure, in combination w/ Ozone. A lot of people write about using it on individual tracks. I don't use it too much that way, but sometimes if a track sounds dull, the BBE always seems to work well.
Prior to purchasing the BBE Sonic Maximizer Plug-In, I wrote to BBE to ask them about the product. I got no response. I kept reading reviews and decided to purchase the BBE Sonic Max Plug-in when I purchased BIAS PEAK LE 5 for my iMac. The BIAS PEAK installed fine, and works fine. The BBE Sonic Maximizer Plug-in installaed just fine. It even shows up on the VST list of effects for the BIAS. But, ever time I try to access the Sonic Maximizer within Bias Peak, I get an error message. So: I wrote to BBE again. And I wrote to BIAS. BIAS responded within hours: something they have done every time I have written to them. In fact, their support was so good prior to my purchase, I felt that BIAS would be a great to work with...and they are. But, BBE has yet to respond. Do you know what BIAS said? Here's the quote: "This is an issue that BBE is aware of and they should have an easy fix for you". Yet< I get no response or help from BBE. Do not buy the product if you want support. I did use install the BBE on my Windows PC and have used it with Sound Forge. It works well. But, unless they help me with support so I can run it on my main work station, my iMac, it is useless to me.
This is a plug-in everyone should have. Both instruments and vocals come out clean and clear. It is more useful than a single equalizer because it is easier and it is quicker to dial in a good sound. I was amazed when I first heard the BBE sound and I think you will be too.
One of the best values I've seen for improving home recordings. Good substitute for processor-hungry EQ plugins. Dials in the highs perfectly.
I have been playing around on a cheap E-MU 0404 and the Cubasis VST program for a year and just couldn't get the "presence" effect that I wanted. I bought a nice GT55 mic and a Presonus tube pre, but still wansn't satisfied. The recordings got clearer but the EQ was still lacking power. The Cubasis VST EQ is quite limited though. I got this program and bam! increased clarity. It is like turning up the presence control on a guitar amp from a muted dull tone. Maybe someone with a full featured software package like Reason 3.0 or Cubase SX 3 doesn't need it, but it is a must for any cheap, entry level package. I feel satisfied enough with my sound now that I'm not going to buy the new, expensive preamp or software that I was considering. I was just after a clear, lively sound and I found it. It is a bit expensive for a single plugin though.
If you don't use the presets, and select more subtle settings (which is very easy to do), this plugin can really help bring out an instrument in the mix. The list price is overpriced though, so look for a deal.
I still laugh at individuals who won't use this plugin. If you won't use it at least consider getting the hardware version. It is incredibley stupid not to use this product...On everything you do musically. You can fiddle around EQ'ing all day and never accomplish the sweetness that this plugin will give you...In a matter of seconds! Awesome!


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Hi my name is Marc Anthony Vidrio. Latin-a-GO-GO is a band that has played for many Different types of events that include Weddings, Outdoor Festival's ,Conventions,Doctor'sConventions, Casino's, Hotel's,the World Famous Zoo and Sea World. We love to interact and play a variety of Latin, Salsa,Cumbia's ,Samba,Oldies, Latin Jazz, Reggage,Jazz,Funk,Rock&Roll,Classic R&R and soft relaxing mellow music.We...
Copyright © 2001-2017 OneWed Inc.
All Rights Reserved.
Copyright © 2001-2017 OneWed Inc. All Rights Reserved.


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civil rights
Tonight, a few lives remembered from the Civil Rights era.
They would have been in their early 60's now. Instead, Denise McNail, Carol Robertson, Addie May Collins and Cynthia Wesley were killed 50 years ago today in one of the worst acts of violence during the Civil Rights Movement. Also remembered tonight, Demetrius Newton, a Civil Rights attorney and Alabama legislator. Continue reading
1963 marked the beginning of The Birmingham Campaign, one of the most influential movements of the Civil Rights Era. In honor of its 50th anniversary this year, Alabama Public Television will be airing "Preserving Justice" on Sunday, October 13th at … Continue reading
The civil rights movement didn't just promote equality for African-Americans. It was also a social justice springboard for other causes. To explore how the movement impacted the fight for LGBT and women's rights, Ray Suarez speaks with George Chauncey of Yale University and Ruth Rosen of the University of California, Davis. Continue reading
George H.W. Bush signed the Americans with Disabilities Act into law in 1990, but more than two decades later, problems still persist in implementing portions of the landmark civil rights measure. Judy Woodruff reports on the struggle states face to fully transition individuals with disabilities out of institutional living. Continue reading
South Africans celebrated Nelson Mandela's 95th birthday by volunteering 67 minutes — the number of years Mandela spent fighting apartheid and serving as his nation's first black president. Kwame Holman reports on how people around the world marked the Nobel laureate's milestone. Continue reading
The George Zimmerman verdict has provoked passionate debate about legal justice and race in the U.S. Judy Woodruff gets reaction from Christina Swarns of the NAACP Legal Defense Fund, New Yorker contributor Jelani Cobb, Jonathan Turley of the George Washington University Law School and Carol Swain of Vanderbilt Law School. Continue reading
Syndicated columnist Mark Shields and Washington Post columnist Michael Gerson join Jeffrey Brown to analyze the political implications of the Supreme Court's major rulings on the Voting Rights Act and same-sex marriage. Continue reading
The Supreme Court decided in a 5-4 vote to strike down a provision of the Voting Rights Act that polices voting discrimination. Ray Suarez gets debate on the fate of the law from Edward Blum of the Project on Fair Representation and Sherrilyn Ifill of the NAACP Legal Defense and Educational Fund. Continue reading
Nearly half a century since his murder, civil rights activist Medgar Evers was honored in a ceremony at Arlington National Cemetery. Gwen Ifill examines the life and legacy of Evers — a World War II veteran and the NAACP's first field secretary in the South — with Jerry Mitchell of The Clarion-Ledger newspaper. Continue reading


text:
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This 5GHz wireless laser mouse is perfect for use at home or in the office.
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Ad ID: 291581


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Publisher's Description+
From capturengo: Dont lose another important receipt or business card again! No keying in data!capturengoTM is a new way to organize your receipts and business cards. The capturengoTM solution empowers your iPhone to do morethis powerful new app combined with our cloud management tool has all the benefits of a business card scanner AND an expense management tool.EXPENSE MANAGEMENT AT ITS BEST! Before you lose an important receipt, simply snap a photo of it, code your expense, add a note and send it to your capturengoTM secure account at www.capturengo.com Reduce the amount of time it takes to process expenses by 50%!ROBUST WEB SITE We key-in important data off your receipts so you can run expense reports without having to retype receipt infojust create an expense report and print. If you have a corporate expense management system, use capturengoTM as a front-end solution for grabbing your receipt images. Gone are the days of copying, taping, stapling and scanning just print out a Receipt Log and you are done! capturengoTM reduces your expense processing time by 50%. BUSINESS CARD CAPTURING capturengoTM has the most accurate solution on the market. Our virtual assistants hand-key data off your business cards and send you a vCard to download. So simple, so accurate.Turn your iPhone into a virtual assistant, download the capturengoTM solution...the perfect accompaniment for anyone on-the-go. see our web demo at www.capturengo.comDownload a FREE limited time TRIAL now!
What's new in this version:
Corrected transmission issue that locked up the outbox and prevented further captures from being sent.First time tutorial screens updated to finger scroll.
read more +


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The Sandlot You're Killing Me Smalls Mens Royal Blue T-shirt
- Adult men's US sizes, standard fit and length.
- Cotton/Polyester blend, medium weight, soft and durable.
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More details
Release Date: 13/06/2008
Originally designed for the PlayStation 2 and released in 2006, Okami now comes alive in an entirely new way on the Wii with its visually stunning art style and beautiful scenic 3D levels that have the appearance and texture of paper scrolls brushed with watercolor-like calligraphy art. Throughout Okami's vibrant and distinctive surroundings, Okami players must use the Wii remote to interact with a dynamic world and cast of characters. Okami's blend of stylised graphics, movements and unique gameplay will result in a rich and dynamic experience never seen before.
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Which Joe gave his name to 'sloppy joes'? We look at five interesting sandwiches and their lexical origins.
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Further reading
6 punctuation marks you might be using incorrectlyRead more
We take a look at several popular, though confusing, punctuation marks.
An A-Z of country name originsRead more
From Afghanistan to Zimbabwe, discover surprising and intriguing language facts from around the globe.
How brothers became buddies and brosRead more
The definitions of 'buddy' and 'bro' in the OED have recently been revised. We explore their history and increase in popularity.


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Tech Off Thread2 posts
Boot performance diagnostics
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Does anyone know how the "boot performance diagnostics" really work.
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Part of the circuitry snacks project: Edible models of functioning electronic circuits. Designed for fun, for geeks, for kids, and for teaching and learning electronics.
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Taken on July 10, 2007


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Citation: Stewart V, van Tilbeurgh H (2012) Found: The Elusive ANTAR Transcription Antiterminator. PLoS Genet 8(6): e1002773. doi:10.1371/journal.pgen.1002773
Editor: William F. Burkholder, Agency for Science, Technology, and Research, Singapore
Published: June 7, 2012
Copyright: © 2012 Stewart, van Tilbeurgh. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors received no specific funding for this article.
Competing interests: The authors have declared that no competing interests exist.
Regulated transcription termination provides an efficient and responsive means to control gene expression [1]. Intrinsic terminators, which consist of an RNA stem-loop followed by a poly-U tract, catalyze termination by disrupting the RNA polymerase elongation complex [2]. In antitermination, an antiterminator stem-loop is mutually exclusive with the terminator stem-loop [1], [3]. In different contexts, formation of the antiterminator stem-loop is governed by a translating ribosome [1], a ligand-binding riboswitch [4], [5], or a signal-responsive RNA–binding protein [3]. This latter mechanism is illuminated by Ramesh et al. [6], who studied antitermination by a broadly distributed class of signal-responsive RNA-binding proteins containing the ANTAR domain.
AmiR and NasR Define the ANTAR Regulators
Studies conducted in the 1990s characterized two related antitermination proteins: AmiR, which regulates aliphatic amide catabolism in Pseudomonas aeruginosa (amiECBRS operon) [7], [8], and NasR, which regulates nitrate assimilation in Klebsiella oxytoca (nasFEDCBA operon) [9], [10]. RNA binding occurs through the carboxyl-terminal ANTAR domain (AmiR and NasR transcription antitermination regulator) [11]. AmiR and NasR RNA binding responds to signal input mediated by the amide-binding AmiC protein [12], [13] and the nitrate-binding NIT domain [14], [15], respectively.
AmiR and NasR target the transcribed leader RNA upstream of the amiE and nasF operons, respectively [12], [14]. Both leaders encode two obvious stem-loop secondary structures: the distal intrinsic terminator (including a poly-U tract) [2], and P1, a proximal structure essential for antitermination (Figure 1A). Further analysis of the nasF operon leader identified three subelements essential for NasR binding and antitermination: the P1 stem; residues A1 and G4 in the P1 loop; and part of the linker region that connects the P1 and terminator stem-loop structures [16]. However, the RNA secondary structure(s) formed during antitermination remained a mystery.
Alternative stem-loop structures in transcribed leader regions from representative operons are shown. Terminator stem regions that participate in alternative structures are in blue, whereas antiterminator regions are in red. (A) NasR-responsive nasF operon leader in termination conformation. Dots indicate the critical residues A1 and G4 in the P1 and P2 loops. (B) Each ANTAR monomer is hypothesized to bind one of the loops, P1 and P2. Stabilizing the P2 antiterminator structure permits transcription readthrough [6] by shortening the terminator stem and separating it from the poly-U tract [2]. (C) LicT-responsive bglP operon leader in termination conformation. RAT is the ribonucleic antiterminator. (D) Each CAT (co-antiterminator) monomer binds to the antiterminator stem. Unusual base-pairing within the antiterminator stem is depicted schematically [25]. Stablilizing the antiterminator structure permits transcription readthrough [22] by shortening the terminator stem and separating it from the poly-U tract.
Although different ANTAR proteins use a variety of signal input domains, at least half contain a two-component signal transduction receiver domain [6], [11]. Receiver function is governed through phosphorylation and dephosphorylation by a cognate signal-responsive sensor kinase [17]. For example, the ethanolamine-responsive EutW sensor kinase controls the EutV response regulator, which mediates antitermination control of ethanolamine catabolism (eut operons) in Enterococcus faecalis [18]–[20]. Other ANTAR proteins, including AmiR, contain a non-phosphorylated pseudo-receiver domain [13], [17].
New Progress with EutV
Ramesh et al. [6] now report a substantial advance toward understanding ANTAR-mediated transcription antitermination. The eut gene cluster contains intrinsic terminators upstream of four genes (eutP, eutG, eutS, and eutA), facilitating the identification of shared sequence features. Each of these leaders contains a terminator along with two upstream stem-loop structures, P1 and P2 [6] (Figure 1B). The eutG leader P2 structure was noted independently by Baker and Perego [20]. Similar structures are present in the amiE and nasF leaders. Strikingly, both the P1 and P2 loops contain the critical residues A1 and G4, identified as essential for NasR-mediated antitermination [16]. Moreover, the distal stem of P2 overlaps part of the terminator proximal stem [6], [20], and thereby forms an antiterminator structure (Figure 1B). This suggests a general model for ANTAR-mediated transcription termination, in which the dimeric ANTAR protein binds simultaneously to structures P1 and P2, stabilizing the P2 antiterminator to enable transcription readthrough. (Indeed, restriction sites introduced into the nasF operon leader [16] destroy the P2 structure, explaining the resultant uninducible phenotype.)
To test this model, Ramesh et al. first constructed a variety of site-specific alterations in the eutP leader, and confirmed the subelements important for EutV binding and antitermination: the P1 and P2 structures; their relative spacing; and the P1 and P2 loop residues A1 and G4 [6]. Ramesh et al. then evaluated the EutV oligomeric states, finding that the wild-type protein required phosphorylation to dimerize. Truncated proteins lacking the receiver domain were isolated as dimers, although the most efficient RNA binding was observed with truncated protein that retains the central coiled-coil. This suggests that the coiled-coil enforces proper spatial orientation between the two ANTAR monomers. Presumably, each ANTAR monomer binds one of the two structures, P1 or P2.
Finally, Ramesh et al. conducted bioinformatic analyses to identify ANTAR target leaders in bacterial genomes. Examples were found in a broad range of species from the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Some of the leaders are adjacent to genes of unknown or uncertain function, whereas many others are adjacent to genes involved in inorganic nitrogen acquisition (ammonium, nitrate, or dinitrogen) or assimilation (glutamine synthetase and associated regulators). Many ethanolamine utilization clusters also were identified.
Together, these results provide concrete hypotheses for the structure and function of both ANTAR domain proteins and their leader RNA targets. Moreover, the association of many ANTAR regulators with aspects of nitrogen metabolism provokes intriguing questions about their physiology and evolution.
Comparison to CAT Domain Antitermination
Proteins of the BglG-LicT-SacY family, present in both gram-negative and gram-positive lineages, control genes for sugar catabolism. These homodimeric proteins contain PRD domains (PTS regulation domain) that place them under control of the phosphotransferase system for sugar uptake [21]. The carboxyl-terminal CAT (co-antiterminator) domain interacts with the RAT (ribonucleic antiterminator) element in the transcribed leader region. The CAT-stabilized antiterminator is mutually exclusive with the terminator structure [22] (Figure 1C and 1D).
The CAT domain is a homodimer of four-stranded β-sheets [23]. Phosphorylation results in massive structural changes [24] that bring the two CAT monomers into proper alignment to interact with a distorted minor grove in the antiterminator hairpin stem [25]. This stem is interrupted by two non-identical asymmetric internal loops, which are recognized similarly by the protein dimer. Sequence analysis suggests that this RNA-binding mode likely is conserved in homologous systems [25].
How Does ANTAR Bind RNA?
X-ray structures are known for three ANTAR domain proteins, each with a different amino-terminal input domain: AmiR, pseudo-receiver [13]; Mycobacterium tuberculosis Rv1626, receiver [26]; and NasR, NIT [27]. Each protein contains an α-helical coiled-coil connecting the two domains, but each structure reveals a different spatial orientation of the ANTAR monomers and a different configuration of the coiled-coil. It is likely that signal is propagated through the coiled-coil to bring the ANTAR monomers into proper alignment for RNA binding [6].
The ANTAR domain adopts a helix-turn-helix conformation, but how it binds RNA is unknown. RNA-binding helix-turn-helix proteins include σ70 region 4.2, which binds the −35 region of promoter DNA as well as 6S RNA [28], and Ffh, which binds 4.5 S RNA in the signal recognition particle [29]. Both of these RNA targets form bulged stem-loop structures, so the binding sites have characteristics of dsRNA.
Future progress in understanding ANTAR-mediated antitermination requires knowledge of ANTAR domain conformation and interaction with its RNA target; conformational shifts mediated through the coiled-coil; and kinetics of RNA binding in relation to transcription-driven formation of the P1, P2, and terminator structures.
- 1. Yanofsky C (2007) RNA-based regulation of genes of tryptophan synthesis and degradation, in bacteria. RNA 13: 1141–1154.
- 2. Peters JM, Vangeloff AD, Landick R (2011) Bacterial transcription terminators: the RNA 3′-end chronicles. J Mol Biol 412: 793–813.
- 3. Henkin TM, Yanofsky C (2002) Regulation by transcription attenuation in bacteria: how RNA provides instructions for transcription termination/antitermination decisions. Bio Essays 24: 700–707.
- 4. Roth A, Breaker RR (2009) The structural and functional diversity of metabolite-binding riboswitches. Annu Rev Biochem 78: 305–334.
- 5. Gutiérrez-Preciado A, Henkin TM, Grundy FJ, Yanofsky C, Merino E (2009) Biochemical features and functional implications of the RNA-based T-box regulatory mechanism. Microbiol Mol Biol Rev 73: 36–61.
- 6. Ramesh A, DebRoy S, Goodson JR, Fox KA, Faz H, et al. (2012) The mechanism for RNA recognition by ANTAR regulators of gene expression. PLoS Genet 8: e1002666. doi:10.1371/journal.pgen.1002666.
- 7. Drew R, Lowe N (1989) Positive control of Pseudomonas aeruginosa amidase synthesis is mediated by a transcription anti-termination mechanism. J Gen Microbiol 135: 817–823.
- 8. Wilson SA, Wachira SJM, Norman RA, Pearl LH, Drew RE (1996) Transcription antitermination regulation of the Pseudomonas aeruginosa amidase operon. EMBO J 15: 5907–5916.
- 9. Goldman BS, Lin JT, Stewart V (1994) Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. J Bacteriol 176: 5077–5085.
- 10. Lin JT, Stewart V (1996) Nitrate and nitrite-mediated transcription antitermination control of nasF (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. J Mol Biol 256: 423–435.
- 11. Shu CJ, Zhulin IB (2002) ANTAR: an RNA-binding domain in transcription antitermination regulatory proteins. Trends Biochem Sci 27: 3–5.
- 12. Norman RA, Poh CL, Pearl LH, O'Hara BP, Drew RE (2000) Steric hindrance regulation of the Pseudomonas aeruginosa amidase operon. J Biol Chem 275: 30660–30667.
- 13. O'Hara BP, Norman RA, Wan PT, Roe SM, Barrett TE, et al. (1999) Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex. EMBO J 18: 5175–5186.
- 14. Chai W, Stewart V (1998) NasR, a novel RNA-binding protein, mediates nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader in vitro. J Mol Biol 283: 339–351.
- 15. Shu CJ, Ulrich LE, Zhulin IB (2003) The NIT domain: a predicted nitrate-responsive module in bacterial sensory receptors. Trends Biochem Sci 28: 121–124.
- 16. Chai W, Stewart V (1999) RNA sequence requirements for NasR-mediated, nitrate-responsive transcription antitermination of the Klebsiella oxytoca M5al nasF operon leader. J Mol Biol 292: 203–216.
- 17. Bourret RB (2010) Receiver domain structure and function in response regulator proteins. Curr Opin Microbiol 13: 142–149.
- 18. Del Papa MF, Perego M (2008) Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis. J Bacteriol 190: 7147–7156.
- 19. Fox KA, Ramesh A, Stearns JE, Bourgogne A, Reyes-Jara A, et al. (2009) Multiple posttranscriptional regulatory mechanisms partner to control ethanolamine utilization in Enterococcus faecalis. Proc Natl Acad Sci USA 106: 4435–4440.
- 20. Baker KA, Perego M (2011) Transcription antitermination by a phosphorylated response regulator and cobalamin-dependent termination at a B12 riboswitch contribute to ethanolamine utilization in Enterococcus faecalis. J Bacteriol 193: 2575–2586.
- 21. van Tilbeurgh H, Declerck N (2001) Structural insights into the regulation of bacterial signalling proteins containing PRDs. Curr Opin Struct Biol 11: 685–693.
- 22. Houman F, Diaz-Torres MR, Wright A (1990) Transcriptional antitermination in the bgl operon of E. coli is modulated by a specific RNA binding protein. Cell 62: 1153–1163.
- 23. van Tilbeurgh H, Manival X, Aymerich S, Lhoste JM, Dumas C, et al. (1997) Crystal structure of a new RNA-binding domain from the antiterminator protein SacY of Bacillus subtilis. EMBO J 16: 5030–5036.
- 24. Graille M, Zhou CZ, Receveur-Bréchot V, Collinet B, Declerck N, et al. (2005) Activation of the LicT transcriptional antiterminator involves a domain swing/lock mechanism provoking massive structural changes. J Biol Chem 280: 14780–14789.
- 25. Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M (2002) Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT. EMBO J 21: 1987–1997.
- 26. Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA (2004) The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis. Structure 12: 1595–1605.
- 27. Boudes M, Lazar N, Durand D, Gaidenko TA, Stewart V, et al. (2012) Structure of the NasR transcription antiterminator reveals a one-component system with a NIT nitrate-receptor coupled to an ANTAR RNA-binding effector. Mol Microbiol. In press.
- 28. Klocko AD, Wassarman KM (2009) 6S RNA binding to Esigma(70) requires a positively charged surface of sigma(70) region 4.2. Mol Microbiol 73: 152–164.
- 29. Batey RT, Sagar MB, Doudna JA (2001) Structural and energetic analysis of RNA recognition by a universally conserved protein from the signal recognition particle. J Mol Biol 307: 229–246.


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