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Mutations in the cytoplasmic linker regions of receptor histidine kinase and chemoreceptor proteins have been shown previously to significantly impair receptor functions. Here we demonstrate significant sequence similarities between these regions in numerous histidine kinases, methyl-accepting proteins, adenylyl cyclases and other prokaryotic signalling proteins. It is suggested that these 'HAMP domains' possess roles of regulating the phosphorylation or methylation of homodimeric receptors by transmitting the conformational changes in periplasmic ligand-binding domains to cytoplasmic signalling kinase and methyl-acceptor domains.
The intracellular response of prokaryotic cells to environmental change is mediated by phosphorylation- and methylation-dependent signalling circuits. Transmembrane receptors of extracellular ligands include molecules with histidine kinase or methyl-accepting functions. Homodimeric histidine kinases, such as Escherichia coli EnvZ, catalyze transphosphorylation of specific histidine residues and the phosphoryl groups are subsequently transferred to specific aspartyl residues, usually on response regulator domains homologous to E. coli CheY [1–3]. Methylation and demethylation of homodimeric chemoreceptors such as E. coli Tar, Tsr, Trg, Tap and Aer are catalyzed by methyltransferases and methylesterases homologous to E. coli CheR and CheB, respectively (reviewed in [4,5]).
Although considerable advances have been made in understanding the functions of these enzymes, much remains to be determined of the mechanisms that regulate the enzyme and pathway specificities. Assistance in these investigations is offered by the finding that histidine kinases and chemoreceptors are often accompanied by regulatory domains that occur within the same polypeptide. Sequence analysis has demonstrated the co-occurrence with histidine kinase and methyl-accepting domains of PAS predicted ligand binding and dimerization domains [6,7] and GAF putative cyclic nucleotide-binding/small ligand-binding domains [8]. These regulatory domains are in addition to the two known domains of histidine kinases, the α-helical dimerization 'A' domain and the α/β catalytic domain similar to the amino-terminal domains of a type II topoisomerase and a chaperone, Hsp90 [9–12].
Sequence similarities have been noted between two receptor histidine kinases (EnvZ and NarX) and chemoreceptor methyl-accepting proteins in cytoplasmic coiled coil-like regions that occur between the C-terminal transmembrane helix and the kinase or methyl-accepting domains [13–16]. However, a recent study was unable to detect similarities between large numbers of histidine kinase sequences and the chemoreceptor coiled coil-like domain [17]. The predicted α-helical regions of chemoreceptors and histidine kinase have been demonstrated to be crucial for proper receptor function [13,18–20]. Consequently, it was our aim to quantify the significance of sequence similarities between these regions of chemoreceptors and histidine kinases, and to investigate whether this region occurs in other multidomain contexts.
Materials and methods
The position-specific, iterative version of BLAST (PSI-BLAST) [21] was used to search a non-redundant version (ftp://ncbi.nlm.nih.gov/blast/db) of current sequence databases. Subsequent analyses included comparison of a hidden Markov model (HMM) with sequence databases using HMMER2 (S. Eddy, unpublished; http://hmmer.wustl.edu/). The domain architectures of signalling proteins were established using SMART (http://coot.emb-heidelberg.de/SMART/) [22].
Results and discussion
A PSI-BLAST [21] search of current sequence databases was used to investigate the putative α-helical region of E. coli EnvZ (amino acid residues 180–234). The search used a threshold for inclusion of sequences in subsequent iterations of E<0.01, where E is the number of sequences expected to be found in the search purely by chance. The first three iterations of PSI-BLAST revealed significant (E<10−3) similarities to similar regions of seven distinct receptor histidine kinases: Bordetella bronchiseptica RisS, Pseudomonas aeruginosa NarX and KinB, Streptomyces coelicolor AfsQ2, Bacillus subtilis YycG, Mycobacterium tuberculosis Rv0982, and Myxococcus xanthus SasS. The fourth iteration revealed significant similarities to numerous histidine kinases and methyl-accepting chemotaxis proteins including four from B. subtilis, TlpA, TlpB, McpA and McpB [23]. After nine PSI-BLAST iterations and convergence, 200 proteins were identified with significant similarities to the putative coiled coil region of E. coli EnvZ. From the domain architectures of these proteins determined using SMART [22], 119 are predicted to be histidine kinases, 66 to be methyl-accepting chemotaxis proteins, eight to be adenylyl cyclases and one to be a PP2C-type serine/threonine phosphatase. Thus, these results indicate that the α-helical region common to chemoreceptors and histidine kinases represents an approximately 50-amino acid conserved domain that is present in multiple signalling contexts. Multiple alignments of this and other domains in prokaryotic signalling proteins are available from the SMART Web site (http://coot.embl-heidelberg.de/SMART/).
We term this homologous domain HAMP (domain present in histidine kinases, adenylyl cyclases, methyl-accepting proteins and phosphatases). Construction of a multiple alignment of HAMP domain sequences allowed additional domain homologues to be detected by comparison of a HMM with sequence databases using HMMER2 and an E value threshold of 0.1; further homologues were detected using reciprocal PSI-BLAST searches and an E value threshold of 0.01 (Fig. 1). Fifteen of the 30 known E. coli histidine kinases and all five methyl-accepting chemotaxis proteins contain a single HAMP domain. There is a strong correlation between the presence of a HAMP domain and transmembrane regions in a protein: in addition to the aforementioned 20 transmembrane E. coli proteins, all of the eight initially detected adenylyl cyclases are predicted to be membrane-bound.
Single HAMP domains are not limited to methyl-accepting, histidine kinase and adenylyl cyclase molecular contexts. Single domains were observed in a putative receptor, Aquifex aeolicus aq_1825 and its homologues, and were found to accompany other signalling domains such as PAS, PP2C-type phosphatases, diguanylate cyclases, phosphodiesterases A, an ammonium transporter and a winged helix-turn-helix-type DNA binding domain (Fig. 2).
HAMP domains do not always occur as single repeats, nor are they restricted to prokaryotes and nor do they always occur in membrane-associated proteins. Soluble histidine kinases such as S. coelicolor SC7C7.03, Neurospora crassa Nik-1 [24] and Candida albicans COS1 [25] contain 12, six and five HAMP tandem repeats, respectively. Prokaryotic methyl-accepting receptors were found to contain a single HAMP. The exceptions to this are halobacterial methyl-accepting receptors [26,27], Rhizobium sp. NGR234 Y4FA and Agrobacterium tumefaciens McpA, that contain a pair of tandem HAMPs.
Mutagenesis studies [13,19,28] provide evidence that receptor histidine kinase HAMP domains negatively regulate receptor activation rather than mediating dimerization, as was proposed for some of the predicted coiled coil regions [17]. Amino acid substitutions at eight positions in the HAMP alignment (Fig. 1) produce constitutively active receptor histidine kinases [13,19,28]. How this is achieved remains unknown. However, we propose from the predicted secondary structure of HAMP of two α-helices (α1, α2) (Fig. 1) that the bihelical HAMP domain of monomeric histidine kinases binds intramolecularly to the bihelical dimerization domain [12] thereby forming a four-helix bundle similar to that seen in dimeric CheA [12]. This internal interdomain association would prevent spontaneous signalling in the absence of cognate extracellular ligands by obstructing molecular dimerization involving pairs of bihelical dimerization domains. This proposal implies that ligand-bound monomeric receptor histidine kinases alter their conformations allowing dissociation of the HAMP domain, and subsequent formation of the four-helix bundle within histidine kinase dimers.
We propose a similar protein interaction function for HAMP domains in chemoreceptors, that is modulated by conformational changes initiated by periplasmic ligand-binding events. Mutagenesis studies of the chemoreceptor Tar [18,20,29,30] demonstrate that the Tar HAMP domain is required to form functional heterodimers, although it is clear that, as with the receptor histidine kinases, it is not necessary for dimerization of the Tar cytoplasmic domains. Eight Tar variants with single amino acid substitutions in the HAMP domain have been shown to be unable to generate CheA histidine kinase activation in vitro [20] (Fig. 1). Thus it would appear that the two HAMP α-helices α1 and α2 adopt two contrasting conformational states dependent on the ligand-bound state of the chemoreceptor. Switching between these states is likely to result from the known piston-like displacement of the transmembrane 'signalling helix' that follows aspartate binding [31]. In this model, aspartate binding results in the bihelical HAMP domain adopting a conformation within Tar homodimers or oligomers that inhibits autophosphorylation of CheA within the Tar-CheW-CheA multimolecular complex.
The presence of HAMP domains in prokaryotic signalling molecules other than histidine kinases and chemoreceptors suggests that similar dual regulatory mechanisms may operate for HAMP-containing catalytic adenylyl cyclase, diguanylate cyclase/phosphodiesterase and sigma-regulating PP2C phosphatase proteins. However, it is apparent that this does not account for the functions of single HAMP domains that are unaccompanied by catalytic domains, such as the HAMP in the putative receptor, A. aeolicus aq_1825. An intriguing possibility is that these domains act in a manner similar to the truncated Tar receptor molecules [29,30] and provide new functional specificities by associating with other receptors.
One further possible function of the HAMP domain is suggested by the presence of multiple, tandem domain homologues in fungal and actinomycete histidine kinases such as C. albicans Cos1 and N. crassa Nik-1 (Fig. 2). Here multiple HAMP domains alternate with an intervening predicted α-helical conserved sequence. Consequently, a serial intramolecular association of these HAMP domains might form a regular superstructure that is necessary for mediating interactions of these proteins with target molecules.
In conclusion, a ~50-amino acid α-helical domain has been identified with statistical significance in multidomain proteins that participate in a variety of signal transduction processes. The HAMP domain differs from 'input' sensing domains such as PAS, and 'output' receiver domains such as CheY homologues, and in receptor contexts is likely to act not as an adapter module, mediating association of signalling complexes, but rather as a sensor of conformational change and inhibitor of constitutive histidine kinase signalling. Recognition that HAMP domains are widespread among prokaryotic signalling proteins should assist in the understanding of the molecular mechanisms that mediate the intricate regulation of prokaryotic membrane-associated signalling arrays [5].


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USC's Dawson, Harris expect to play vs. Syracuse
USC was without three key defensive contributors last week, but at least two of them appear to be on the way back.
Linebacker Lamar Dawson and cornerback Torin Harris both pronounced themselves fit and ready to play at Syracuse after battling injuries during training camp.
Dawson thought he'd be back last week but "didn't want to rush things." He said he was about 95 percent recovered from a reported calf injury.
Harris hasn't played since Sept. 24, 2011, when he suffered nerve damage in his shoulder. He sat out spring practice, then injured a hamstring a week into training camp.
"I kept getting knocked down, but I just had to find a way to get back on the field," Harris said. "I've been through a lot worse than just a hammy pull."
Dawson is listed as the staring middle linebacker on the official team depth chart. Harris is listed as the second-team right cornerback. How much they play against Syracuse remains to be seen.
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A widget that accesses your phpBB forum and displays recent posts on your WordPress page.
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Whatever happened to logic?
Here's a good question from Elizabeth Smiley at American Thinker. …How can you use the very same expressions you claim are so bothersome? It defies logic. The were many signs about science at the womyn's march, yet those same people … Continue reading
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Greenbuild Conference and Expo 2015
Washington, D.C., November 18-20, 2015
The U.S. Green Building Council's Greenbuild International Conference & Expo convenes the industry's largest gathering of representatives from all sectors of the green building movement. Three days of extensive educational programming, workshops, a vast exhibition floor and ample networking events provide unrivaled opportunities to learn about the latest technological innovations, explore new products, and exchange ideas with other professionals.
Greenbuild includes over 100 educational sessions featuring world-renowned speakers, LEED workshops, off-site educational sessions, and tours of local green building sites. Past speakers have included Former Secretary of State Hillary Clinton, Archbishop Desmond Tutu, President Bill Clinton, General Colin Powell, Paul Hawken and David Suzuki.
The 2015 show will be held November 18-20 in Washington, D.C. at the Washington Convention Center. The event will offer an excellent opportunity to engage leaders in a region known for green building and responsible forest management. Greenbuild's theme this year is "Monumental Green", to celebrate the leadership of the green movement.
Greenbuild is the finest sponsorship opportunity with FSC in the U.S. FSC's prominent presence on the Expo Floor, our high-profile annual FSC Leadership Awards Ceremony, and the sponsorship benefits make this an exclusive opportunity.
If you have any questions about Greenbuild or the Leadership Awards, please do not hesitate to contact Amelia Harris, Business Development Manager (email@example.com, +1-612-353-4511). We would love to work with you.
Thank you to our 2014 Greenbuild Sponsors
Platinum Sponsor - The Home Depot
Gold Sponsor - Kimberly-Clark Professional
Silver Sponsors:
SCS Global Services
Roy O Martin
F.D. Sterritt
Mendocino Redwood Company
US Floors


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You missed it This great deal for Bapi Italian Ristorante is over. But don't despair, we've got more great deals every day.
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Dr. Boyan Majstorovic - General & Family Dentistry!
When you visit Alpha Dental Centre, your smile and health is our top priority. Kelowna Dentist Dr. Boyan Majstorovic and his entire team are dedicated to providing you with the personalized, quality dental care that you deserve. Dr. Majstorovic has been practicing family dentistry in B.C. since 1996 and is proud to serve patients from Kelowna, Penticton, Summerland and all of British Columbia.
At Alpha Dental Centre you will experience all that modern dentistry has to offer, including a comprehensive list of general dentistry, restorative treatments, cosmetic dental services, and Invisalign to meet the needs of the whole family. Our goal is to assist each patient in achieving and maintaining long term dental health and a beautiful smile. Some of the services we provide include:
View Our Dental Specials!
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Spyder Women's Tres Chic 3-in-1 Systems Ski Jacket
The Spyder Women's Tres Chic 3-in-1 Systems Ski Jacket is a luxurious, versatile and warm piece that performs on and off the hill. The Tres Chic is a supple 360º full stretch waterproof and breathable fabric that will combat the worst of conditions without sacrificing mobility and comfort. The silky, warm, lightweight PrimaLoft® insulated liner will keep you toasty warm without packing on the bulk. More often than not we hear, "this jacket is too thin, surly it can't be warm." Yes it can! Thick and bulky doesn't mean warm it just means, not flattering. This flattering, low profile, jacket is built to keep you warm in the cold. Once waterproofing, breathability and warmth have been addressed the rest is just icing on the cake. And there is a lot of icing on the cake. Removable heavy weather hood so if you're done on the hill and want to hit the town you can remove some excess material. On the inside you have a zip pocket, lipstick pocket and a satin mini purse with chamois lens wipes. In the left sleeve is a data card pocket and for comfort there are adjustable sleeve cuff zippers. The Tres Chic is tres magnifique.
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- Age
- Adult
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- Female


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Moni.ai is an interactive virtual assistant which allows you to voice control your business.
IFTTT is a service that lets you create powerful connections with one simple statement: "if this then that".
With Moni.ai's Channel on IFTTT you can start putting the Internet of Things to work for you. In order to add voice to your favorite products and services, follow these 3 easy steps:
Create a new recipe and select Moni.ai as your "this" Channel
Come back to Moni.ai and start performing actions with your voice (be sure you are signed into the Moni.ai website or app before testing your IFTTT recipes!)
Detailed Instructions: How to create a recipe using the Moni.ai Channel on IFTTT.
As the Internet of Things expands we need an easier way to interact with our technology. Processes are getting more and more complex and we need a way to manage them. That is why Moni.ai is a virtual assistant that speaks your language. Watch the video to learn how Moni.ai can help you automate your business processes. After watching the video start building your first Voice Channel.
Moni.ai enables us to control technology with our most natural form of communication, our voice. In order to let you talk with Moni.ai in natural language we developed the Moni.ai apps that are available on Android and iOS. Download the app on iTunes or on Google Play now! Or add Moni.ai as a contact on Skype.
Be part of the voice revolution and control your business with your own Voice Channel: Companies and developers can implement custom capabilities into Moni.ai. This allows you to define new ways to interact with your customers or voice control your business by automating internal processes (e.g. business analytics, reporting, etc.).
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Our neuronal network is unique and inspired by the human brain. It can 'predict' results instead of 'finding' results. The outcome is a self-organizing architecture that is unlimited in content, features, languages, etc.


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Real-Time Platform for Machine Data (RTTK)
Capture, Analysis, Distribution, and Visualization of Real-Time Streaming Machine Data Learn about RTTK with two videos RTTK Intro Video RTTK Intro & How It Works Video The Real-Time RTTK Platform provides a set of tools, services, and frameworks for monitoring and control of real-time distributed...
Virtual REX (VREX) – Simulate Impractical Complex Radar
Electromagnetic sensors include a "front-end" that generates and receives the electromagnetic waves through a subsystem known as a Receiver/EXciter (REX). They also include a "back-end" that performs signal and data processing, typically implemented in software. These separate subsystems must...
Real-Time Engineering Services
FishEye Software provides high-technology software engineering for embedded real-time systems to defense, government, and commercial customers. Our engineering team averages over 25-years real-time experience in software development, integration, and test for real-time industries. We're...
Real-Time Platform – Real-Time Data Capture Analysis Distribution Fisheye's Real Time Platform (RTTK) provides a platform for capture, analysis, distribution complex real-time systems data streams. The Real Time Platform manages the data flow and meta-data for systems comprised of varying...
Synthesizing the World's Real-Time Data
Real-Time Engineering
An engineering team averages over 28 years' experience in real-time systems, software and firmware development, integration & test. [more]
Real-Time Platform (RTTK)
Fastest infrastructure for real-time machine data capture, analysis & distribution. [more]
Virtual Radar REX (VREX)
Simulate impractical complex electromagnetic targets and environments in real-time affordably.[more]
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Software, firmware, and systems engineering services and real-time products for defense, government, & commercial industry. [more]
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Radar Analyst – Huntsville, AL
FishEye is hiring at least one junior to mid-level radar analyst with UEWR & Cobra Dane background and MatLab experience. Familiarity with the new UEWR software and hardware (UV2000 and 9.x software) is a...Read More
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A: Advantages and Disadvantages of archiving text and HDF5 data formats. HDF5 is a data storage format that can store a variety of datatypes and is designed for flexible and efficient I/O and for high volume and...Read More
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UEWR Operator in Huntsville, Alabama
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The primary objective of this core is to facilitate the scientific and academic advancement of Project Leaders. The core is designed to provide the Project Leaders with the guidance and/or assistance they need to navigate the Institutional Animal Care and Use Committee and to access university-sponsored core facilities and instrumentation, editorial assistance (with grant and manuscript writing), and biostatistical assistance (e.g., with experimental design, data analysis). Ensuring access to these state-of-the-art services is key to supporting the research efforts of the Project Leaders and ensuring that the objectives of the COBRE are met. The Core Leader will work with Project Leaders to identify and remove any banriers that might impede research or faculty development.
Specific Aim 1. Establish multiple-color flow cytometry capabilities in the existing Flow Cytometry Core Facility. We propose to acquire a BD LSRFortessa cell analyzer (BD Biosciences, Franklin Lakes, NJ) and to provide expert technical and managerial oversight for instrument use. Multiple-color flow cytometry analysis currently is needed by all of the COBRE projects. This instrument meets the needs of those projects and expands the capabilities of the existing core facility by providing significant enhancement in multiple-color flow cytometry analysis. The technical and managerial structure is already in place and provides expert, high-quality flow cytometry analysis for investigators at UAMS and surrounding campuses (Arkansas Children's Hospital and Research Institute, University of Arkansas at Little Rock, University of Central Arkansas, and others).
Specific Aim 2. Provide Project Leaders with resources and support needed for project and faculty development. Core personnel will provide Project Leaders with direct assistance in developing animal-use protocols, writing manuscripts and grant applications, and biostatistical analysis and will facilitate Project Leaders'use of existing shared facilities or resources. To facilitate efficient use of Project Leaders'time and resources, and of the facilities and instrumentation currently in place on the UAMS campus, Core B will assist the Project Leaders in identifying and accessing needed resources and support services available through the various offices at UAMS. This will remove barriers that could limit productivity, and it will provide learning experiences that Project Leaders will use throughout their careers.
The services described in Core B will directly impact the biomedical research proposed in the COBRE application by providing the investigators with the tools necessary to carry their projects to fruition. Additionally, the resources will provide investigators with life-long learning experience that will enhance their professional development into biomedical researchers who can compete successfully for research funding to make discoveries that directly impact public health.
|Kovak, Matthew R; Saraswati, Sarika; Schoen, David J et al. (2014) Investigation of galectin-3 function in the reproductive tract by identification of binding ligands in human seminal plasma. Am J Reprod Immunol 72:403-12
|Pawar, Snehalata A; Shao, Lijian; Chang, Jianhui et al. (2014) C/EBP? deficiency sensitizes mice to ionizing radiation-induced hematopoietic and intestinal injury. PLoS One 9:e94967
|Winchell, Caylin G; Graham, Joseph G; Kurten, Richard C et al. (2014) Coxiella burnetii type IV secretion-dependent recruitment of macrophage autophagosomes. Infect Immun 82:2229-38
|Nakagawa, Mayumi; Coleman, Hannah N; Wang, Xuelian et al. (2014) IL-12 secretion by Langerhans cells stimulated with Candida skin test reagent is mediated by dectin-1 in some healthy individuals. Cytokine 65:202-9
|Maddukuri, Leena; Ketkar, Amit; Eddy, Sarah et al. (2014) The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DNA polymerase kappa. Nucleic Acids Res 42:12027-40
|Kennedy, Joshua L; Schroeder, Nikhila; Palacios, Thamiris et al. (2014) Fifty-five-year-old man with chronic yeast infections. Allergy Asthma Proc 35:415-22
|Fortune, Danielle E; Lin, Yi-Pin; Deka, Ranjit K et al. (2014) Identification of lysine residues in the Borrelia burgdorferi DbpA adhesin required for murine infection. Infect Immun 82:3186-98
|Coleman, Carrie B; McGraw, Jennifer E; Feldman, Emily R et al. (2014) A gammaherpesvirus Bcl-2 ortholog blocks B cell receptor-mediated apoptosis and promotes the survival of developing B cells in vivo. PLoS Pathog 10:e1003916
|Groshong, Ashley M; Fortune, Danielle E; Moore, Brendan P et al. (2014) BB0238, a presumed tetratricopeptide repeat-containing protein, is required during Borrelia burgdorferi mammalian infection. Infect Immun 82:4292-306
|Beenken, Karen E; Mrak, Lara N; Zielinska, Agnieszka K et al. (2014) Impact of the functional status of saeRS on in vivo phenotypes of Staphylococcus aureus?sarA mutants. Mol Microbiol 92:1299-312
Showing the most recent 10 out of 16 publications


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Mitsubishi Lancer estate (1993 to 1997)
also known as - Mitsubishi Mirage estate
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Roma director Franco Baldini insists Aurelio Andreazzoli won't necessarily leave the bench this summer and shoots down links with Massimiliano Allegri.
This morning the Corriere della Sera reported that there was already a three-year deal in place with the Milan Coach, but both clubs released statements categorically denying the story.
"There is no deal with Allegri," added director general Baldini on Sky Sport Italia ahead of tonight's match with Fiorentina.
"Everyone seems to assume Roma will change Coach, but Andreazzoli is doing very well. This is our last call for a Champions League spot. We'll keep trying until it becomes mathematically impossible, but are facing a very strong side who have had an excellent season.
"I expect it to be a very entertaining game tonight with two teams who make possession their primary strength."
Keep up to date with the latest news and action from Spain's Primera Division with Football Espana - from the team behind Football Italia.


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Belgium Highlights
If Belgium's spotlight on the European stage is a little dim, it's only because its people are rarely boastful. This slow-burning country has more history, art, food and architecture packed into its tiny self than many of its bigger, louder neighbors.
A rich and bubbling vat of beer, chocolate, oil paint and bureaucrats, Belgium gives off the heady smell of the bourgeoisie. But stir the pot a little and you'll find an 'artificial state' made up of two parts Germanic Flemings to one part Celtic-Latin Walloons.
Top Belgium AttractionsSee all
- 1 Magritte Museum (Musée Magritte)
- 2 Manneken Pis
- 3 Ypres
- 4 Grand-Place
- 5 Brussels Royal Palace (Palais Royal de Bruxelles)
What's Happening Now
Two Countries, One Trip: How to See More on Your ...
February 2016
Carnival Celebrations Around the World
January 2016


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Env: Windows XP, Bon Echo nightly build 20060731, and JAWS 7.1.
Problem: When running FF2 with the javascript alert (see attached file)the contents of the popup is not read by JAWS.
Steps to recreate:
1. Download the attached file, start JAWS and FF2.
2. Ins + down arrow till you get the the example heading of "Javascript Popup current Time"
3.Tab to the button "Display the Current Time" button.
4. JAWS announces only the title of the Popup window which is "Javascript Application" and stops there. It should read the information inside the window which is "The current time is ...."
Expected results: FF2 & JAWS should read the string within the Popup window.
Created attachment 231455 [details]
*** Bug 346708 has been marked as a duplicate of this bug. ***
Does this work with WindowEyes? If so, file a bug against JAWS.
Created attachment 268002 [details]
Much simpler testcase
Indeed this is only a problem with JAWS, but I will try to use describedby or some other way to get this spoken.
I'm unable to work around this. JAWS should notice that it's ROLE_ALERT and read all the static text in the dialog.
Tim, can you take this one up with Freedom Scientific using the "Much simpler testcase" file. Once there's a bug filed there you can close this one as INVALID.
I've filed it with them, but I'll keep this around until I hear from them.
This is working correctly in Window-Eyes but not in JAWS. Marco, can you make sure it is filed with Freedom Scientific?
Joanie, this is something to try in Orca.
Not a Firefox bug.
Aaron, confirmed that JAWS does not read the text inside the Alert. However, in looking at the window with AccExplorer, I notice that there are actually three children, the first of which is an invisible text object, the second one is the text object containing the word "hello", the third one being the OK button. What's up with this invisible first child?
The invisible child is made visible when there is a different kind of alert. JAWS should just skip over invisible objects. I don't think that's the issue, but I guess I could be wrong.


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< back to Lynne's profile page
Agent Ratings for Lynne Sawyer
(26 reviews) Overall star rating: 5.0
Past 3 months: N/A
Closed clients: 5.0
Past 3 months: N/A
Closed clients: 5.0
Ratings Breakdown:
5 stars(26)
4 stars(0)
3 stars(0)
2 stars(0)
1 stars(0)
Showing All Reviews (26) Show only: 5 stars| 4 stars| 3 stars| 2 stars| 1 star| show all
Carole C.
Condo seller in Smyrna
Lynne is really a full-service agent. There are always a hundred tiny ridiculous things that pop up in any transaction, and Lynne never hesitates to address all of them promptly. Our last sale was a little dragged out by the buyer's lender, and Lynne called for updates and kept the communication going.
Previous review from Carole C.
I really appreciate Lynne's quick response time. She is always on the ball for me and gives me not only the information about the property I am looking at, but also the comps and tax and any other information that helps me make buying decisions.
Previous review from Carole C.
Lynne was extremely responsive and thoroughly explained every step of the process we were going through. I always felt in control of or at least totally involved in the process. She also offered great advice about values and pros and cons.
Sharon T.
Home buyer in Decatur
Lynne Sawyer is a terrific agent with your best interests at hand.Her dedication is devoted to helping you through the whole buying process side-by-side. She continues to help even after you've closed on your home. My husband Willie and I will highly recommend Lynne. Thanks sooooo very very much Lynne.
Previous review from Sharon T.
Lynne Sawyer is a terrific agent with your best interests at hand.Her dedication is devoted to helping you through the whole buying process side-by-side.She continues to help even after you've closed on your home.Willie (my husband) and I will highly recommend Lynne.Thanks sooooo very very much for everything, Lynne.
Previous review from Sharon T.
Lynne Sawyer is a terrific agent with your best interests at hand.Her dedication is devoted to helping you through the whole buying process side-by-side.She continues to help even after you've closed on your home.Willie (my husband) and I will highly recommend Lynne.Thanks sooooo very very much for everything, Lynne.
Teresa D.
Home buyer in Loganville
Lynne was exceptionally knowledgeable and always made herself available, even the day before Thanksgiving. She was honest and truthful about everything. She took the time to look at homes we inquired about before showing them to us, thus saving us time. She educated us and held our hands through the whole process. we could never have done this without her
Rebecca L.
Home buyer in Decatur
Lynne's a great agent with deep knowledge of the market and the business. Her candor -- pointing out trouble spots when she could've just as easily let them slide for the sake of a quick sale -- is as refreshing as it is rare. Teaching me, along the way, things to look out for. As a first-time homebuyer, I was glad to have her on my team. She's a Master of her craft. As disappointed as I was after my first offer fell through, she turned around and had 6 great places to show me. The first one we toured that day was is the place I now call home. We closed in a month. Wouldn't have happened without her coaching me through the process.
Karen M.
Townhome buyer in Decatur
Lynn made my buying experience a pleasure. Her knowledge and expertise made a huge difference in where in bought my home. She has an awesome winning personality and I would recommend her to anyone who wants to take a little stress out of moving. She has a wealth of information and you can trust that she has your best interest in mind.
Sandra L.
Home buyer in Stone Mountain
Lynne was a heaven sent. Throughout the process Lynne was my Realtor, my therapist and my paralegal. She educated me on processes I knew nothing about even though this was my second home purchase. Lynne made the entire experience fun and help me see the bigger picture when I started freaking out. She is knowledgeable and one of the kindest souls I have had the opportunity to meet. If I know anyone looking for a home I will refer them to her and she will be my Realtor as long as I an in GA.
Nora F.
Home buyer in Decatur
Lynne was not only knowledgeable about real estate in general, but so enthusiastic about looking at properties. She made the whole process a lot of fun. Because she has been attending home inspections along with her clients for years, she was able to point out pros and cons in each property that most realtors miss completely. And we know, because we had four different agents show us houses. But once we went looking with Lynne, we didn't waste anymore time with anyone else.
Previous review from Nora F.
Extremely helpful. Guiding through all the processes and knows the area like no other.
Brooke H.
As a first time home buyer, I was dreading the process. I had heard how difficult and tedious it could be. Thank goodness my Mother In Law recommended Lynne. She was so easy to work with!! Lynne was available every time I needed her, down to earth and fun to be around, and VERY knowledgeable! We closed on our house a week ago and she is still helping us with a septic tank issue. It was so great to work with her and I recommend her 100%!
Levi S.
Home buyer in Tucker
As first time buyers we lacked the knowledge to complete the purchase of a home on our own. Coming recommended from a friend, Lynne bridged that gap, and guarded against pitfalls that I'm sure we'd have encountered without her. She proved to be more than just an agent, but an ally and friend in the process as well. We'll use here in the future and would recommend anyone else do the same.
Doina P.
Townhome buyer in Atlanta
Lynne is very professional, reliable, and knowledgeable. She was always available to address my questions and concerns and made the home purchase a stress-free process. Thanks, Lynne!
Anne L.
Home buyer in Stone Mountain
Lynne really knows her stuff! She directed us through the various technicalities and stresses without fail, and always alerted us to the next step. And this is AFTER I insisted that I didn't want a Buyer's agent and would use my own bank and vendors! Well, I ended up very grateful for Lynne's reference to her tried and true trusted vendors, who saved the contract my vendors nearly ruined for us. Lynne is a gifted woman, professionally and personally. She was able to recognize and work with my own strengths and weaknesses, and guide us through the necessarily complicated process of transferring property ownership. Whether buying or selling, we will return to Lynne for the benefit of her skills and personality!
Home buyer in Atlanta
Lynne really made the entire transaction smooth. She was always available to answer any questions.
Adrainne T.
Home buyer in Lithonia
Lynne Sawyer is very professional, reliable, liberal and last but not least you can trust that what ever she states is exactly whats going to be done. She has brought PURE JOY to me & my family of 3 in our purchase of our very first HOME. The entire process was PERFECT so my advice to all--if you want a HOME contact LYNNE SAWYER! My 5 STAR friend for life.
Franck d.
Home buyer in Snellville
No review submitted
Previous review from Franck d.
Lynne is definitely the best agent I have ever worked with. She was diligent and prompt to react, not to mention always providing useful information or update by phone or email. I would definitely recommand her to anybody else
Autumn L.
Home buyer in Atlanta
Lynne was an superb agent. She pointed out the pros and cons of each property and helped us decide the best place for us. I can not imagine a better agent. I recommend her to all my friends and family. I chose her as my agent because she was highly recommended by a friend of mine.
Joseph H.
Home buyer in Snellville
Lynne worked with me and my whole family while I was deployed oversees. I know that my situation was a difficult one but Lynne helped me through it. She is a great realitor and friend. I would definitely suggest her to anyone I know is buying a home in the area
Home buyer in Pine Lake
Lynne Sawyer's personal interest and dedication to helping me find my house was invaluable. Her patience and and professional advice saw me through my first home purchase and made the process a fun and educational experience.
Home buyer in Stone Mountain
Lynne was always available to address any questions and concerns. Her energy and personal attention made this purchase possible for me. Thank you, Lynne!
Gwen F.
Home buyer in Lithonia
lynn Sawyer was the best, she was a blessing to my family, she was sent from. GOD. I want to say thank you so much. GWENDOLYN FAVORS.
Evelyn F.
Townhome buyer in Alpharetta
We thought that Lynne was an excellent representative of Zip Realty. She was extremely organized and professional. We have had other transactions with realtors and Lynne was by far the best we have worked with. She was extremely patient at times and that was greatly appreciated. We have had a great experience with Zip Realty starting with the excellent web site..Thank you.......Chris Fecarotta
Eric M.
Townhome buyer in Atlanta
I had been through 3 other agents, and Lynn was the first one to show me properties that were within my budget and with the amenities I wanted. She was very helpful and not the slightest bit pushy. I would defenitly recommend her to friends looking for homes.


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List 100 items on eBay / Amazon ( long term cooperation )
This project received 49 bids from talented freelancers with an average bid price of $124 USD.Get free quotes for a project like this
Project Budget$30 - $250 USD
Total Bids49
Project Description
I'm looking for *****AN EXPERT***** on listing items on eBay / Amazon.
We will start with 100 items, but as mentioned I'm looking for a long term cooperation. if those 100 items done good, we will continue to list more ( I have endless amount of items to be listed about 100,000 at least )
- the most important thing, is that you should be an EXPERT on this, that knows how to built titles, do a good description, edit pictures, etc.
Skills Required
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


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My Spencer Storage Twin Bed is one untouchable value! Three stylish colors and three storage drawers make this panel bed a must have! At only $499, it's an absolute steal!
Colors Available:
- Black
- White
- Cherry
Product Details:
- Bed includes headboard, footboard and 2 wood rails
- Framing is constructed of solid hardwood, MDF and plywood.
- Moldings are constructed of poplar wood
- French dovetailed front drawers and English dovetailed back drawers
- Kenlin metal glides with built-in drawer stops
- Twin bed has three storage drawers, one on each side and one in the footboard
- Mattress not included
Spencer Storage Twin Bed
Twin Storage Bed
Shipping Info
Shipping Info
In stock items usually ship within 10 days via Bob's Delivery Service. Lamps, Rugs, Bed Pillows, Bed and Pillow Protectors, select Kids Chairs and select Mattresses ship via FedEx through my Bob's Value Express program when purchased alone or in combination with each other. If purchased along with furniture, Bob's Value Express items will ship via Bob's Delivery Service. Mattresses shipped via FedEx / Bob's Value Express require special set up. Click here for details. Bob's Gift Cards ship FREE via USPS Ground Shipping or via FedEx 2 Day Shipping for only $9.50. A Bob's representative will call within 24 hours of placing an order to schedule Bob's Delivery Service, arrange a Pick-Up date or give FedEx delivery information.
Please note:
**Due to limited quantities, most Outlet merchandise must be delivered within 2 weeks, pending available delivery dates. Some Outlet items may require transfer within distribution centers requiring up to 3 – 4 weeks for availability.
**Mattresses shipped via FedEx under the Bob's Value Express program do NOT qualify for removal of old mattress and foundation.
For more information about shipping and delivery charges, click here.
- What is Goof Proof Plus?
- Description
My Spencer Storage Twin Bed is one untouchable value! Three stylish colors and three storage drawers make this panel bed a must have! At only $499, it's an absolute steal!
- Specs
Short Description Twin Storage Bed SKU 20026383-config Size Twin Collection Spencer Warehouses Taftville, Shorewood, Aberdeen Color Black Height 52" Width 44" Depth 80" Material Wood Seat Height Stool Height Finish Style Traditional Motion Length


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More Videos...
Sexy Subaru WRX Visits Maxxsonics HQ! Hot Import Nights Chicago
http://www.maxxsonics.com Aaron Vaccar stopped by Maxxsonics HQ with his heavily upgraded 2004 Subaru WRX Sti on the way to Hot Import Nights HIN at Soldier Field in Chicago. This vehicle is packed with performace and audio upgrades including the MB Quart Discus DWI304 subwoofers, DSC4125 and DSC500.1 amplifiers, DKH116 coaxial and DSH216 component speakers. This vehicle is also featured in the August 2009 issue of Performance Autosound Magazine http://www.pasmag.com . Aarons' Myspace: http://www.myspace.com/acvmotorsports ACV Mailorder Service for Performance Parts: http://www.acvmailorder.com Maxxsonics: http://www.maxxsonics.com MB Quart: http://www.mbquartusa.com Mike Stiers This video features beats from Mister K.A. Check out all of his beats at http://www.misterkabeats.com Powered by Maxxsonics MB Quart Hifonics Crunch Autotek
2006 Subaru STi - GT45R - 45psi
Inside AMR Performance: http://youtu.be/z1NWgmXh6hw www.fastsuby.com www.automasteramr.com / www.amrperformance.com "Like" us on facebook. Link: http://www.facebook.com/pages/AMR-Performance/121395404588072 2006 Subaru STi - GT45R - 45psi www.automasteramr.com
How To Upgrade Your Turbo
In this episode of MCM, the boys do a turbo swap on the Moogaru, a 2009 Subaru Forester XT. You can grab mad MCM gear here (including some of the music from this episode): http://www.mightycarmods.com/collections/all iTunes version of the TRANSFORMER EP by MOOG (featured in this episode) available here http://itunes.apple.com/au/album/transformer-single/id542490506 And check out the turbo Supporting Mods episode here: http://www.youtube.com/watch?v=y-bcPNJzwtA Discuss this episode on the MCM Forum in this thread: http://forums.mightycarmods.com/showthread.php?17010-***-NEW-EPISODE-turbo-Upgrade Also something to note around Mighty Car Mods: we are normal guys and are not trained mechanics. We like to make interesting car mods and show you how we've gone about it, but we can't promise that anything we show you will work for your particular car, or that you won't harm yourself, someone else, your car or your warranty doing it. Please be safe, be responsible and unless you know what you're doing, do not fool around with very serious machinery just because you've seen us make it look so easy. Talk to a qualified mechanic if you are in any doubt.
How to swap a Subaru turbo
Subiesport Magazine swaps a turbo in its 2007 Legacy GT project car. Includes some great tips for anyone doing this popular upgrade.


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Carlo Rivetti opens the doors to his brand Stone Island, headquartered in Ravarino, Italy. During a lengthy tour Carlo reveals the philosophy of a company that has always been founded on research and experimentation. Revealed is the passion and the deep know how that turns ideas and visions into garments and collections that are always innovative and in the vanguard. Enjoy!


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Prince will be playing 3 shows at this years event
Music fans can expect a host of big names at this year\'s edition of the Montreux Jazz Festival in Switzerland, with three concerts by Prince as well as shows by Leonard Cohen,Sting and Joe Cocker
, organisers announced on Thursday.
\"Prince wanted to come, he even asked himself. We don\'t know what he\'s going to do, but by definition it will be great,\" said the festival\'s new director Mathieu Jaton.
The July 4-21 event on the shores of Lake Geneva is the 47th edition of the festival, which has retained its jazz label despite expanding its repertoire over the years.
It is the first event since iconic founder Claude Nobs, who was widely respected in the music business, died in a skiing accident in January aged 76.
A traditional magnet for international stars, the festival will also see an 80th birthday concert by Quincy Jones, who worked closely with Nobs from the beginning of the festival.
Others on the eclectic bill include Paolo Conte, Diana Krall, George Benson, Deep Purple, Kraftwerk, ZZ Top, Ben Harper, Brian May, Marcus Miller, Wyclef Jean, Randy Crawford, Cat Power, Green Day and Woodkid with Orchestra.
To accommodate the star-studded list, this year\'s 18-day edition is two days longer than usual.


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Press Conference: Rally on the Green Asphalt Plant
37-98 Railroad Avenue
Long Island City, NY 10305
Senator Gianaris will be attending the Rally on the Green Asphalt Plant with Assemblymember Nolan, Councilman Van Bramer and Community Board 2 Chair Conely. This rally is being held in response to a plan to construct an asphalt plant in Long Island City without allowing the public or the Department of Environmental Conservation the required period of time to comment on it.
How a Bill Becomes LawLearn More
Senator has new policy idea
Idea is drafted into a Bill
Bill undergoes committee process
Senate and Assembly pass bill
Bill is signed by Governor


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Welcome to City Walls
Many pubs take their name from a point of interest somewhere vaguely in the area, but not The City Walls: the only way into this large and lively bar is through the city wall itself. An archway through Stirling's 16th-century defences leads you into a fascinating conversion of what was once an ironmongers' warehouse. The combination of ancient and modern attracts a classy crowd, whether they be tourists, families, locals, office workers, sports fans or weekend party lovers. Whoever you are, there's plenty of room - including the upstairs beer garden where you can enjoy your Belhaven Best and Belhaven Black with a view over King's Park.


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TEDx speakers talk gloves, war
Have you ever competed to build the tallest structure of mini marshmallows and spaghetti, topped with a jumbo marshmallow – with only one hand? If you answered yes, you probably attended the recent TEDxUCIrvine ideas conference.
UCI professors and students shared their creations, ideas and discoveries at the event, called "Collaborating Forward." The intermissions were filled with creative "labs," including the marshmallow spaghetti structure activity. Here is a sampling of the talks given:
Vincent Xu, a UCI student studying business and economics, discussed Ambience, a university club he founded that has expanded to 15 chapters at schools across the nation.
Ambience is a club for people who want to tell a story and express themselves using lights.
"I grew up with two left feet for my whole life," Xu said.
He loves melodic trance music, so he decided to become a "glover." Glovers wear white gloves with lights in the fingertips and perform in the dark. They also use other props with glow-in-the-dark elements.
"Gloves are the paintbrushes; imagination is the canvas," he said.
Gloving is a subculture that evolved from rave culture, he said. People often illogically equate gloving and drugs, because they connect raves and drugs. Not true, Xu said. He promotes light shows as a drug-free art form.
Al Valdez, a national expert on gangs, sociology and youth violence and prevention, teaches an introduction to gangs class at UCI. He is a UCI alumnus and chairs Westwood College's School of Justice in Anaheim.
He spoke about the value and necessity of forgiving others.
Valdez, who has five sons, recalled when he got the call no parent wants: It began with "I regret to inform you," he said. "You can't imagine the horror that went through me."
His son Joshua was shot in the head by a gang member and survived.
"I wanted an eye for an eye. I wanted revenge," he said. "I had to forgive the 18-year-old boy who hurt my son," he said.
"I learned a secret – help others. Join a fellowship of service. Align yourself with like-minded people, then you become the agents of change," he said.
Annessa Stagner, a doctoral candidate in the history department, talked about discoveries she made researching her dissertation, "Defining the Soldier's Wounds: U.S. Shell Shock in International Perspective."
Stagner, who delved into World War I soldiers' diaries, said if we mind our history, we can see a pattern. The headlines of war in 1921 are the same as today, she said.
"For many, the first World War was a nightmare that imprinted itself on soldiers in the form of shell shock," she said. Doctors thought they could fix soldiers so they no longer had problems. It didn't work.
Soldier trauma and suicide were a big problem then, and they're a big problem now.
"War looks different today, but war still produces trauma and our medicine still fails to cure it," she said. "Science and technology can't wipe away consequences of war."
Mahtab Jafari, director of UCI's Pharmaceutical Sciences Undergraduate Program and associate professor, pharmaceutical sciences, spoke at the conference
While not an expert in stress management, Jafari wanted to help her students who were stressed and using various medications to cope, so she created Life 101, a 10-week stress management course at UCI.
Jafari started the class after hearing from a number of students who were using Adderall to stay up and then sleeping medications to catch up with sleep.
When Life 101 was first offered in winter of 2013, 105 students registered. The purpose of the class is to change behavior and promote healthy lifestyles.
These skills are as important – maybe even more important – as physics or calculus, she said.
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The "Teacher" appears.
The "teacher" can be an actual person who can help you and . . .
it can also be something that people miss all the time . . .
Our willingness to accept a lesson.
Perhaps a lesson we've heard many times but we simply weren't ready . . .
To ACCEPT IT!
In that moment of acceptance . . . Your teacher has appeared.
The problem is that our ego can get in the way of that.
It loves to give us excuses and give us ways to transfer ownership by attributing this to the other person having a problem or attitude or simply not understanding us.
Fear has a way of getting in the way of the lesson too.
According to Dr Piers Steel its fear that's at the root of our procrastination and don't we have a way of putting off dealing with things?
Things that could be considered a "teacher" in their own right!
Perhaps your "teacher" has been staying away because you are too busy to accept them?
Some daily "thinking time" to reflect, ponder and plan might encourage the teacher to come knocking.
I hope you'll find a way to accept your "teacher" this year . . .
I can't think of a better way to accelerate your efforts and rust proof your personal growth!
For those of you who would like to attack the new year differently, I would highly recommend my Playbook For A Successful Year webinar on January 10th at 1:30 Eastern. Here's the link with all the detailshttp://www.instantpresenter.com/PIID=EA52DD82834F Oh and I intentionally set the date for this for that magical moment when our New Year's resolutions are starting to lose some of the initial momentum!
To hire Paul as your personal coach or to train your team, click here


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I live next door to a couple who from time to time have tremendous arguments, and get into fights. The funny thing is that separately they seem quite nice people, and I get along with the husband tolerably well. If he has a violent streak, he certainly never shows it in my presence. Well, the other day he revealed to me that he usually kept a gun in his house for self-defence, and that the gun had gone missing. Did I know anyone, he wanted to know, who could sell him a new one? I do in fact know several people who deal in firearms (all quite kosher and legal, of course) and I could easily get them to sell him one. But should I?
Robin Cook writes: Yes, of course. It is for self-defence, isn't it?
Yes, that's what he says, but how can I be sure of that ? I mean, who's to say that in one of these domestic imbroglios he might not in the heat of the moment draw his gun and shoot his wife dead? I'd feel a bit guilty then.
Robin Cook writes: Well, what you have to do in these circumstances is get him to make a solemn declaration that he will use his gun for peaceful purposes only and not kill his wife. Then if he shoots his wife, or indeed anyone else, it won't be your fault.
I'm a bit worried about this idea of using a gun peacefully. How can a gun be used for peaceful purposes?
Robin Cook writes: Oh, easily. For target practice. For sport. For rabbit hunting. For starting a race. For deterring a burglar. As an intriguing paperweight... You might say that guns have more peaceful than violent purposes!
But what if he turns out to need the gun for criminal purposes? What if he is really an armed robber in pursuit of his trade? I would then be aiding and abetting a criminal act.
Robin Cook writes: Yes, that is a real consideration. There must always be an ethical dimension in our strategy, and it does you credit to remember it. On the other hand, if you don't sell him a gun, someone else will. So sell it to him, and stop shilly-shallying around!
But is that true? Will someone else always sell him a gun? And even if they do, is that justification for doing something that may lead to bloodshed?
Robin Cook writes: I have an idea that will allay your very real and respectable fears. Your only reservation, if I read you right, is that if you sell a gun to your neighbour, he may do some damage to his wife. Is that not correct?
Robin Cook writes: Then what you must do is go to the wife and point out that since her husband is getting armed, so, for her own protection, should she. It would also make sense to sell her a weapon of slightly higher firepower than the one you are selling to the husband.
Robin Cook writes: Partly so that she can protect herself. But partly so you can go back to the husband, tell him that he is outgunned on the home front, and suggest that he trade in his new weapon for an even newer and better one.
Won't this lead to an escalation whereby I shall find myself selling ever more powerful guns to both partners?
Robin Cook writes: Let us hope so. For it will lead not only to greater peace next door, but also to very good profits for you. This is what ethics is all about.
Robin Cook will be back soon. Keep those ethical problems rolling in!Reuse content


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Make Money With Best Seed
Are you spending $80-$125 per bag of seed corn? Why, certainly not to make more profit per acre. Many competing brands cost $20-$75 more per bag than the top quality hybrids sold by Best Seed Company.
On an average of $50 more per bag you could be spending as much as $17 per acre to plant one of our heavily advertised competitors. $2 corn means you have to make 8.5 bushels more per acre just to pay the seed cost! The research results show they will not do this.
Another way to look at the great difference is what could you buy for $17 per acre. Another chemical or insecticide application. Or, @$125 per ton, $17 would buy a split application of Liquid Nitrogen and an additional 30# of nitrogen per acre! I have never had a competitor say their $125 hybrids could out yield mine with a 30# nitrogen advantage. But the cost per acre is the same! How they justify these outrageous seed costs is beyond me.
Money is too tight to not consider what you could do with your money, rather than some seed company.
This Site Best Viewed at 800x600 or Higher Resolution.
Copyright © 2015 Best Seed Company, Inc.


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May 25-29, 2017
Singletrack Summer Camp at the Trans-Sylvania Epic Mountain Bike Race: May 25-29, 2017
Come back in 2017! for Singletrack Summer Camp at the Trans-Sylvania Epic!
A new look and format await you at the 2017 Trans-Sylvania Epic.
Now 5 days of stages! Arrive Wednesday, May 24 for a late day welcome gathering and the awarding of the first leaders jerseys of 2017 Ride, rinse, relax, repeat
New looks to classic stages. A remote start is back. Some new food options. And some more fun planned at camp!
Duo is back too! Bring a friend and spend week riding incredible trails together
A free tent site is included with every entry; upgrade to other on-site lodging or RV parking
All the other great pieces that makes our riders want to come back year after year will part of TSEpic again.
And of course all the #EastCoastRocks you want!
Registration Basics:
Solo, Duo, our non-racing "Experience" and "Epic Team" options exist at the Trans-Sylvania Epic. The details of these options and what they include is explained on the Categories Page of our site. After choosing your category you can continue to choose options for lodging, food. All registrations include a campsite as part of registration with on-site lodging upgrades available.
The Trans-Sylvania Epic offers all participants a:
Fully supported mountain bike vacation
Setting that allows allowing you to relax amidst the deep dark forest greenery, complete with camp lake, cold spring fed streams and swimming pool between stages.
Base camp style approach allowing you to bring anything and everything you need to one location for the whole event – spare parts, your coffee maker, back up bike, extra gear, the rest of the family, your favorite teddy – pack 'em all
Renowned mix of subpurb trails and courses, "The best mix of any stage race!" Colt McElwain
Singletrack Summer Camp atmosphere you get nowhere else!
For more information on Stage Info, Maps, Daily Schedule, Additional Services, Prize List, Photos, Media coverage and more, please visit the links on the right hand sidebar.
And don't hesitate to call or email with more questions!
"Due to your support our events have raised more than $20,000.00 for PA trails and the effort to launch the PA Interscholastic Cycling League. Please help us to continue to support these efforts by coming out and having some fun at our events and by donating directly to the PA League PAMTB.ORG."
Some of our great partners!
Thanks for joining us this year!
Relive the memories with our 2016 race coverage!
TRANS-SYLVANIA, PA (June 3, 2016) – The final stage of the NoTubes Trans-Sylvania Epic brought thrills and spills on a tough, rocky course made more difficult with a downpour in the middle of the day. Battles for the overall general classification (GC) wins didn't go…
TRANS-SYLVANIA, PA (June 2, 2016) – Stage 4 of the NoTubes Trans-Sylvania Epic was a wet and wild ride at R.B. Winter State Park, a mainstay of the event with some new trails and more miles added for this edition. A steady rain through most…
TRANS-SYLVANIA, PA (June 1, 2016) – Today was Enduro Day at the NoTubes Trans-Sylvania Epic – a day when descending prowess is key for every racer. While the elite solo general classification contenders tried to keep the rubber side down and their hopes of overall…
TRANS-SYLVANIA, PA (May 31, 2016) – Today's stage featured some of the signature trails of the NoTubes Trans-Sylvania Epic, including classic Eastern rocky singletrack that inspired racers to give it their all. Despite the challenging terrain, the top riders fought until the end and kept…


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Thanks to this product + Melatonin from the same company Now Foods
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Thanks to this product + Melatonin from the same company Now Foods
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A gentle alternative to antidepressants. Perfect for PMS!
stomach pain if take it an empty stomach..is it my problem only?
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stomach pain if take it an empty stomach..is it my problem only?
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I use it for my mood swings and appetite and so far I feel much better. My mother uses it for her nerves and since she is taking 5-HTP she has not taken a pill for her nerves yet! Amazing result. Sadly, I cannot say it works for the appetite, at least not for me, perhaps after another month of taking.
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muy buenooo!!
Uplift my Mood feeling Great when waking up.
Posted by MyPersonalReview on Jan 13, 2014
I took it before I sleep. it uplifts my Mood feeling Great when waking up. As I am a MASSIVE Believer in Alternate Medicine and wanting to contribute in the best way of possible to the world, please help to click my username and view my page in Iherb to see all the reviews I have written of those purchased products I ordered in Iherb. ( It may takes days to months before I write the review as I need to try them thoroughly ensuring the preciseness and accurate experience taking in comparison to the thousands of page of research papers I have read which is the main reason leading me to buy the product from Iherb because simply, I want to contribute myself in this humanity world.
good product
Posted by Reviewer2133762 on May 26, 2014
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Boiler Pressure - how to top up the system?
Discussion in 'Boiler Forum' started by CanuckMark, Oct 20, 2013.
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We have a semi-protected (breakwall) area on Lake Erie that our rec department wants to get pricing on for creating a "beach" area. It is a relatively small area (60' x 60). I cannot find any typical sections of beach design anywhere on the internet. If anyone has a good sketch/drawing or link of a beach typical section it would be helpful. We would like to find out costs prior to bugging the USACE and ODNR. Thanks for any help you could pass along.
This is my "GO FISH" project of the week, as it may or may not (more likely) come to fruition.


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- 80 PLUS BRONZE Certified
- 100 - 240 V 47 - 63 Hz
One of the worst expensive Power Supplies 01/23/2014
This review is from: Thermaltake toughpower W0117RU 750W ATX12V / EPS12V SLI Certified CrossFire Ready 80 PLUS BRONZE Certified Active PFC Power Supply
Worked great when it worked.
Only worked for about a year or so and that was with the Thermaltake 150 Graphics power supply running in concert to take the graphics load off the Toughpower. I would not call it a Toughpower, I would call it a No-Power as it does not work and I have a power supply from NewEgg that cost me 40 bucks that has worked for the last 4 years without fail, my toughpower is a paper weight and has been for the last 5 years or so. Which is rediculous. It lived just long enough to be out of warranty.
Other Thoughts:
I expected much more from Thermaltake. I have now switched to all Corsair Power supplies. Gold Series.
Did you find this review helpful? Yes / No Thanks for the valuable feedback you provided!


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Why is t you f*cking right whiners can't discuss the President without bringing up race?You are a perfect example of why our country is in such a sorry state and why we have a moron for president.
There are two basic reasons our country elected your anti-American light skinned negro.
1 - morons like you (Democrats) who think he defeated his "composition" came out in force to vote
2 - the corrupt leftwing media lies for Obama the pos
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<quoted text>
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Former 'Hawk improves on own meet record
Day three of the Kansas Relays saw former Jayhawk Scott Russell return to Lawrence to defend his 2006 javelin title.
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Hi Friends of Little Kids Rock,
As you know, Little Kids Rock transforms children's lives by restoring and revitalizing music education in our nation's public schools. We are happy to say that we just held our largest single teacher training ever in Dallas Texas last week. We trained a total of 58 teachers and equipped them with 900 brand new instruments and they are going to help us reach over 6,000 students in Dallas this year!
Check out what the Dallas Morning News had to say about us - http://www.dallasnews.com/sharedcontent/dws/news/localnews/stories/DN-music_28met.ART.Central.Edition1.26e893a.html
Our goal this year is to purchase and place 8,000 brand new instruments into our schools around the country. We have already placed 6,500 so far. If we reach our goal of 8,000, we will have DOUBLED the number of instruments we gave away just last year.
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Thanks for your interest in our kids and our work!
The Team at Little Kids Rock
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Two crescents: New moon, old Venus
Filed under pretty pictures, amateur astrophotos, Venus, the Moon, many worlds, amateur astronomers
Here is the image of just the Moon and Venus crescents compared.
Original image data dated on or about January 2, 2014.
This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. For uses not allowed by that license, contact us to request publication permission from the copyright holder
: Pal Varadi Nagy
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Hf Acid 7664 39 3 Hydrofluoric Fluic Solution - Chinaprice: contact company for price
Other Names: hydrofluoric acid, HF acid, Fluic acid; Hydrofluoric acid solution
CAS No. 7664-39-3
Molecular Formula HF
EINECS No.: 231-634-8
Appearance: colorless and transparent liquid, with a strongly irritating smell
Purity 30%~70%
UN No.: 1790
Hazardous Grade 8
HS code: 2811110000
Place of origin Hubei Province, China
Annual output 5000MT
Payment Terms: L/C, T/T, Western Union
Delivery Detail: In 15 days on receipt of deposit
Packing: (1) 25Kg/200Kg drums (2) Isotank with net weight 20000kg.
Loading capacity of 20'GP 21MT
Delivery Port Yangsi Port, Wuhan City or China Main port
Properties: The product is a colorless, transparent liquid. It has a strongly pungent odor. It is very corrosive and strongly toxic. White fumes can rise from it in air. Boiling point, 19.5°C.
Application: widely used in salts fluoride, fluoroplastics, fluoro-rubber, fluoro-medicine, agricultural pesticides industry etc.
Please feel free to contact me for more informations.
We supply it with good quality and competitive price, feel free to contact me for more informations.
Skype: lydia9098
Phone: +86-02783315521
Mobile: +8613469960910
Company Contact:
- Posted By: Wuhan Gains Chemical Co.,Ltd
- Phone: 8602783315521
- Address: Building 8th,Fuze Science Park, Gutian 3rd Road, Wuhan, China
- Email:
- Website: http://www.gainschem.com
Published date: November 6, 2013 -
- Business Description: involved in international trade import export of goods, products, and services
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Sodium Fluorosilicate Silicofluoride Ssf 16893 85 9Chemicals - Wuhan Gains Chemical Co.,Ltd - China - November 6, 2013 - contact company for price
Introduction of Sodium Fluorosilicate : Product Name Sodium Silicofluoride (Sodium Fluorosilicate) SSF CAS No. 16893-85-9 Molecular Formula Na2SiF6 Molecular Weight 188.06 Appearance : white crystal or crystalized powder. Purity 98.5% Place of origin...
Sodium Fluoride Sf 7681 49 4 NafChemicals - Wuhan Gains Chemical Co.,Ltd - China - November 6, 2013 - contact company for price
Product Name : Sodium Fluoride SF CAS No.: 7681-49-4 Molecular formula: NaF Molecular weight: 42 Appearance : a white powder or crystal. Purity 99% Place of origin Hubei Province, China annual output 10000MT Hazardous Grade 6.1 UN NO 1690 Packing : P...
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Product name: Aluminium fluoride;Aluminium trifluoride; CAS NO: 7784-18-1 Molecular Formula: AlF3 Molecular Weight: 83.98 Appearance: White crystal or powder. Purity: 90% Packing : Plastic woven bag lined with plastic bag, net weight 25kg. Loading ca...
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