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- Most bookedSchlosshotel Mailberg
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"This is a unique hotel with a long history. It belongs to the Order of the Maltese and was built in 1628. Unfortunately he restaurant was closed due to renovation but the town has a fantastic alternative only 5-10 waking minutes down the road. The rooms are unique. We stayed in room 5. I ..."
A Traveller, Oct 2016, CA
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"I chose the hotel because of its location as I was just travelling from Austria to Czech Republic. As much as the hotel is aimed at the casino and spa visiting clientele, I felt looked after by the staff and was actually surprised how nice the room and the food in the restaurant were. Staff were ..."
A Traveller, Jun 2016, GB
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- Penzion KaplankaNo Guest Reviews yet
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"We've visited Therme Laa - Hotel & Spa twice during a posting in Vienna. The hotel is easily accessible from Vienna also by train. The hotel offers a free shuttle bus to and from the nearby train station in Laa. The hotel has a high standard and a rather good restaurant. The therme complex is t..."
A Traveller, Aug 2015, GB
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"clean and tidy room but remember to get the key of the main entrance of the hotel, otherwise you cant get out of the hotel at night."
A Traveller, Jan 2017, AS
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"Room and service very comnfortable. Great location. Use of spa facilities reportedly free in the morning. Best breakfast I'd had in Czech Republic hotels! Omelette chef on standby! Hotel had own brewery and winery! Only downside was couldnt get teh wifi to work in public areas. A few other guest..."
A Traveller, Aug 2016, GB
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I am a cat person.
I often leave my cell phone at home or keep it turned off: I can make plans without endless texted revisions. Besides, I think my mental health requires long periods of uninterrupted spacing out.
Music matters. I front a band. I'm always writing a new song in the back of my head.
I try to see movies in the theater. No plasma screen with surround sound can match the experience of sitting in the dark with strangers.
People think I'm younger than I am.
I shoot black and white film on an old Leica. I would rather peer through a loupe into a grainy contact sheet than flip through hundreds of images on my computer.
I enjoy old things. History interests me and I like to approach life with a little pageantry.
I used to be a contrarian but, nowadays, I keep my contrarianism on a tight leash, preferring to turn my attentions to higher interests.
I have a problem with authority figures, especially ones in uniforms. I know- boring. I'm getting over it.
I tend to overdress. I like surprises. My parties are well-attended and tend to go late into the evening.
I have spiritual interests- I read the tarot.
I have a lot of female friends but am striving to achieve gender parity in my inner circle.
I think professional sports are interesting on a sociological level and that they can be aesthetically beautiful but I don't watch them.
I'm a bike-rider.
I lived in New York for a long time. I still think it's unfair when the bars close at two.
I have a diastema. I have no tattoos or piercings but I have some interesting scars.
Making and recording sounds. And songs.
2.) "Five Easy Pieces", "The Third Man", "The Royal Tannenbaums", "Being There", "Two Lane Black Top", "Tree's Lounge"
3.) Cate Le Bon, The Beach Boys, The Kinks, The Flaming Lips, Judee Sill, Gary Numan, Nick Drake, Fleetwood Mac, Big Star, Roxy Music, The Stooges, David Bowie, Nick Cave, The Hold Steady, Elvis Presley, Serge Gainsbourg, Black Sabbath, Van Halen, the usual AOR favorites...
4.) Mexican, Thai, raw vegetables, Cafe Flore (on Sunset)...
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subset: commoncrawl


text: On the Road to Nowhere September 20, 2010August 19, 2014 A: Yes Written by The Puppet Hi kids!. I'm so much more than a tube sock. I write words and stuff. Not a fan of bleach or teenage kid feet. Website Q: Does a bear shit in the woods? Roscoe P. Coltrane, hearing the call The better part of this is that the photo radar installation 100 yards away from this cop is dismantled as of today. Like this:Like Loading... Related Post navigation Same old Cardinals Ohio University : We're pussies


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Lyon need to overturn a 2-1 deficit but they head into this match in dodgy form after losing three of their last four games in all competitions. Remi Garde's side did get back to winning ways on Sunday with a convincing 4-0 victory at Bordeaux, and they currently sit second in the Ligue 1 table and trail the big spending leaders Paris Saint-Germain by three points.
Visitors Tottenham head to France in good form and are unbeaten in their last ten league games. The north Londoners sit fourth in the Premier League and are currently on course to qualify for next season's Champions League. Spurs goalkeeper Hugo Lloris returns to his former side although it is not clear if he will play after boss Andre Villa-Boas picked second choice Brad Friedel for the first leg.
KICK-OFF: Thursday, 6pm
Tottenham Hotspur 2 (Bale 2) Lyon 1 (Umtiti), Europa League, Round of 32, February 2013
Lyon have beaten Tottenham at home before, securing a 1-0 win in the first leg of a round two 1967-68 European Cup Winners' Cup tie, and a repeat scoreline would send the French side through.
That 1-0 win over Spurs is Les Gones' only home win against an English side. They have drawn five and lost one of the other six matches.
Lyon have won their last four European home games, beating APOEL 1-0 in the last 16 of last season's Champions League and recording victories against Sparta Prague, Athletic Bilbao and Hapoel Kiryat Shmona in the group stage of this season's Europa League.
Tottenham are unbeaten in Europe this year after seven matches, with three wins and four draws.
Spurs are unbeaten in their last four Europa League away matches, although the last three have been draws and their last victory was a 4-0 success at Shamrock Rovers in December 2011.
Tottenham won the inaugural UEFA Cup in 1971/72 and reclaimed the trophy in 1983/84, and could become the fourth side to win this competition for a third time, along with Juventus, Inter Milan and Liverpool.
Lyon to win: 2.34
Tottenham to win: 3.3
Draw: 3.5
TV: Live on ITV4 at 6pm, highlights on ITV4 at 6pm on Friday.
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The prognostic significance and value of cyclin D1, CDK4 and p16 in human breast cancer
DOI: 10.1186/bcr3376
© Peurala et al.; licensee BioMed Central Ltd. 2013
Received: 5 September 2012
Accepted: 10 January 2013
Published: 21 January 2013
Loss of the retinoblastoma protein tumor suppressor gene (RB) coding for a nuclear phosphoprotein that regulates the cell cycle is found in many human cancers and probably leads to disruption of the p16-cyclin D1-CDK4/6-RB pathway. Cyclin D1 is known to activate CDK4, which then phosphorylates the RB protein, leading to cell cycle progression. p16 inhibits CDK4, keeping RB hypophosphorylated and preventing cell cycle progression. The significance of these three markers, cyclin D1, CDK4 and p16, for breast cancer and carcinogenesis is nevertheless still controversial.
The material consisted of 102 formalin-fixed human breast cancer samples, in which cyclin D1, CDK4 and p16 expression was evaluated immunohistochemically. The amounts of cyclin D1 mRNA present were analyzed by quantitative real time PCR.
High cyclin D1 expression statistically significantly correlated with lower tumor grade, estrogen and progesterone receptor positivity and lower proliferation activity in breast tumors and increased breast cancer-specific survival and overall survival. Tumors with high cyclin D1 protein had 1.8 times higher expression of cyclin D1 mRNA. CDK4 expression did not correlate with cyclin D1 expression or the survival data. p16 expression was associated with Human Epidermal Growth Factor Receptor 2 (HER2) negativity and increased breast cancer-specific survival and disease-free survival. No statistical correlations between cyclin D1, CDK4 and p16 were found.
Cyclin D1 was associated with a good breast cancer prognosis but functioned independently of CDK4. High cyclin D1 expression may be partially due to increased CCND1 transcription. p16 correlated with a better prognosis and may function without CDK4. In conclusion, it appears that cyclin D1, CDK4 and p16 function independently in human breast cancer.
CDK4 activity is deregulated in many human tumors [9]. It has been shown that CDK4 and CDK6 are dispensable when it comes to driving the essential cell cycle, but they are required in specialized tissues and possibly for achieving higher levels of proliferation [10, 11]. CDK4 has been shown to be absolutely crucial for various oncogenic transformation processes, suggesting that many cancer cells may be addicted to high CDK4 activity [9].
Cyclin D1 is a product of the CCND1 gene [12], which is today considered a well-established human oncogene [5]. The gene and its product have been extensively examined in cases of cancer and there is significant evidence of their involvement in breast, lung, colon, bladder and liver cancers, melanoma, oral squamous cell carcinomas and mantle cell lymphoma [4, 5, 13]. The amplification of CCND1 and the overexpression of the cyclin D1 protein frequently occur in breast cancer, although overexpression of the protein is not always due to gene amplification [4, 5]. Estrogen uses cyclin D1 as one of its target genes to mediate its mitogenic effects [12]. Cyclin D1 expression has been shown in previous human breast cancer studies to correlate with positive ER status [12, 14], the protein being predominantly expressed in the well-differentiated, low-grade, slow-growing subtypes of breast cancer [12].
The product of the CDKN2 gene, p16, acts as a tumor suppressor [15], while inactivation of the gene has been found to be a common event in nearly half of all human cancers studied [16]. Normal proliferating cells do not express significant levels of p16 prior to extensive rounds of cell division, which may suggest a late-stage antiproliferative role for p16, as in the senescence of replicative cells [17]. The activation of p16 expression can be triggered by DNA damage, oncogenic stress or physiological aging [7]. The significance of p16 overexpression is not fully understood, however, and it has been associated with both better and poorer prognosis of cancer. p16 has been associated with a poor prognosis for neuroblastoma and cervical, ovarian, breast and prostate tumors [6]. Recent studies have shown a correlation between overexpression of p16 and both an infiltrative tumor border pattern in breast cancer [16] and the basal-like phenotype [18-19].
In the present work, we assessed the expression of cyclin D1, CDK4 and p16 in human invasive ductal mammary carcinoma samples, correlated the findings with the known prognostic factors for breast cancer and investigated the correlations of these three markers with survival functions. As has been said, all the factors studied here contribute to the RB pathway, and the aim was to reveal the significance of cyclin D1 and its regulators, CDK4 and p16, and their interrelations in human breast cancer.
Patient and tumor material
Patient characteristics
n= 102
Estrogen receptor (ER)
Progesterone receptor (PR)
Adjuvant treatment
Immunohistochemistry (IHC) and scoring
Tissue processing
The surgical specimens were placed in formalin prior to embedding in paraffin for subsequent routine light microscopy and immunohistochemical analysis of cyclin D1, CDK4 and p16. The immunostaining was carried out as follows. Three micrometer sections were deparaffinized and treated with TRIS/EDTA with pronase for epitope retrieval. After this the sections were incubated with blocking solution (EnVision Detection System, Dako, Glostrup, Denmark) to block nonspecific binding of IgGs and then incubated with polyclonal antibodies to cyclin D1, diluted 1:25 (M3635, Dako North America Inc., Carpinteria, CA, USA), CDK4, diluted 1:100 (DCS-35: sc-23896, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), and p16, non-diluted (CINtec, mtm Laboratories AG, Heidelberg, Germany). The color was developed with diaminobenzidine tetrahydrochloride (DAB) (EnVision Detection System, Dako). All the steps were followed by washes with tween/phosphate-buffered saline (PBS). Finally, the sections were lightly counterstained with hematoxylin before analysis by light microscopy and scoring as for DAB staining. For negative controls, the sections were incubated with PBS instead of the primary antibodies. Immunohistochemical analysis of the expression of PHD1-3, HIF-1α and HIF-2α in the same cohort has been described before [21].
The cytoplasmic and nuclear stainings were evaluated individually for all three markers, and the intensities of both were scored on a scale from 0 to 3, where 0 = negative, 1 = week, 2 = moderate, and 3 = strong staining. Also, the percentage of nuclei was assessed in each case and scored on a scale of 0 to 100%. Evaluation of the staining of PHD1-3, HIF-1α and HIF-2α has been described before [21].
The cut-off points for Ki-67 were: negative < 5%, + 5 to 15%, ++ 16 to 30%, and +++ > 30%. Human Epidermal Growth Factor Receptor 2 (HER2) was considered positive in IHC when the result was either ++ or +++ (reflecting moderate to strong intensity in all or almost all tumor cells), and gene amplification status was determined using chromogenic in situ hybridization. Cancers with six or more gene copies were considered HER2 positive [22].
The cut-off value for estrogen and progesterone receptors (ER and PR respectively) was 10%. Tumor samples with ER or PR expression less than 10% were considered negative and the others positive.
RNA extraction, cDNA synthesis and quantitative real time PCR (Q-PCR)
RNA was extracted from the paraffin sections of breast tumors with NucleoSpin FFPE RNA/DNA (Macherey-Nagel, Düren, Germany). A total of 100 ng of RNA was used for cDNA synthesis performed with iScript (Bio-Rad, Hercules, CA, USA). The amount of cyclin D1 mRNA relative to 18S rRNA in the samples was analyzed by Q-PCR performed in a Stratagene MX3005 thermocycler with iTaq SYBR Green Supermix and ROX (Bio-Rad) and the primers qHs3CCND1For 5'-GCTCCTGGTGAACAAGCTCAA-3', qHs3CCND1Rev 5'-TTGGAGAGGAAGTGTTCAATGAAA-3', Hs18SFor 5'-GACTCAACACGGGAAACCTC-3' and Hs18SRev 5'-AGCATGCCAGAGTCTCGTTC-3', respectively.
Statistical analyses
The statistical analyses were carried out with SPSS 17.0 (SPSS Inc., Chicago IL, USA). The clinical characteristics were expressed as percentages. The Chi-square test or Fisher's exact test was used to evaluate the associations and correlations, as appropriate. A two-tailed P-value was used in all the analyses, and a P-value < 0.05 was considered statistically significant. Disease-free survival, breast cancer-specific survival and overall survival were analyzed by the Kaplan-Meier method using Cox regression. Hazard ratios (HR) and 95.0% confidence intervals (95.0% CI) are indicated. Disease free survival was considered as the primary endpoint.
For the statistical analyses, the staining results for the proteins studied were combined to form a positive and a negative group. Cyclin D1 was considered positive when the proportion of stained nuclei was > 40% and when the intensity of the nuclear staining was strong and negative in other cases. CDK4 and p16 were scored as negative when ≤ 2% of the nuclei were stained and the nuclear intensity was negative or weak and as positive in other cases.
All the 102 patients were women and their median age was 59 years (range 28 to 87 years). The prognostic factors are described in Table 1. The majority of the tumors were of low tumor stage (64.7% were T1), nodal negative (56.9%), and steroid receptor positive (76.5% were ER positive and 65.7% PR positive). A total of 17.6% of the tumors were HER2 positive. Grades I to III were represented in almost equal numbers. About 40% of the cancers were intermediate or highly proliferated. The adjuvant treatments provided for the patients are described in Table 1. Only 12% of the patients did not receive any adjuvant therapy (Table 1).
The purpose here was to correlate the immunohistochemical expression of cyclin D1, CDK4 and p16 with the main clinical prognostic factors for breast cancer: tumor stage, nodal status, tumor grade, steroid receptors (ER and PR), HER2 status and proliferation rate.
Cyclin D1, CDK4 and p16 expression in breast cancer in relation to clinicopathological variables
Cyclin D1 - n (%)
Cyclin D1 + n (%)
Cdk4 - n (%)
Cdk4 + n (%)
P16 - n (%)
p16 + n (%)
21 (52.5)
41 (73.2)
14 (56.0)
39 (65.0)
27 (64.3)
32 (62.7)
13 (32.5)
12 (21.4)
10 (40.0)
14 (23.4)
10 (23.8)
15 (29.4)
3 (7.5)
3 (5.4)
1 (4.0)
5 (8.3)
2 (4.8)
4 (7.8)
3 (7.5)
0 (0.0)
0 (0.0)
2 (3.3)
3 (7.1)
0 (0.0)
20 (51.3)
34 (61.8)
15 (65.2)
34 (56.7)
26 (61.9)
29 (59.2)
19 (48.7)
21 (38.2)
8 (34.8)
26 (43.3)
16 (38.1)
20 (40.8)
9 (22.5)
20 (35.7)
11 (44.0)
14 (23.3)
11 (26.2)
17 (33.3)
10 (25.0)
23 (41.1)
6 (24.0)
22 (36.7)
14 (33.3)
16 (31.4)
21 (52.5)
13 (23.2)
8 (32.0)
24 (40.0)
17 (42.5)
Estrogen receptor (ER)
18 (45.0)
3 (5.4)
6 (24.0)
14 (23.3)
10 (23.8)
12 (23.5)
22 (55.0)
53 (96.6)
19 (76.0)
46 (76.7)
32 (76.2)
39 (76,5)
Progesterone receptor (PR)
18 (45.0)
13 (23.3)
8 (32.0)
22 (36.7)
15 (35.7)
18 (35.3)
22 (55.00)
43 (76.8)
17 (68.0)
38 (63.3)
27 (64.3)
33 (64.7)
30 (75.0)
48 (85.7)
21 (84.0)
48 (80.0)
28 (66.7)
47 (92.2)
10 (25.0)
8 (14.3)
4 (16.0)
12 (20.0)
14 (33.3)
4 (7.8)
8 (20.0)
12 (21.4)
6 (24.0)
9 (15.0)
8 (19.0)
10 (19.6)
10 (25.0)
24 (42.9)
11 (44.0)
21 (35.0)
15 (35.7)
18 (35.3)
7 (17.5)
13 (23.2)
2 (8.0)
16 (26.7)
10 (23.8)
9 (17.6)
15 (37.5)
7 (12.5)
6 (24.0)
14 (23.3)
9 (21.4)
14 (27.5)
Although about 70% of the tumor samples showed nuclear CDK4 expression, no statistically significant correlation was found between this and any of the clinico-pathological factors studied (Table 2). There was, however, a tendency for CDK4 expression to correlate with a high tumor grade (P = 0.156) (Table 2), but not with any of the survival functions (data not shown).
The interrelations between the above three markers were analyzed, as they are linked together in the cell cycle, but no statistically significant correlation was found between cyclin D1 and CDK4 or between CDK4 and p16. There was a trend for a positive correlation between cyclin D1 and p16, however (P = 0.145). No significant correlation among all three factors was observed in a three-variable logistic regression model.
When we considered the relations between the expression of cyclin D1, CDK4 and p16 and hypoxia-inducible factor (HIF) prolyl 4-hydroxylases PHD1, 2 and 3, HIF-1α and HIF-2α, the expression of which we had studied earlier in the same samples [21], we found positive correlations between p16 and both PHD1 (P = 0.032) and PHD2 (P = 0.027) and a correlation between CDK4 positivity and PHD3 negativity (P = 0.031).
Disruption of the p16-cyclin D1-CDK4/6-RB pathway occurs frequently in many human cancers [2], and we investigated here the expression profiles of three distinct factors involved in this pathway: cyclin D1, CKD4 and p16. Overexpression of cyclin D1 has earlier been associated with breast cancer subtypes that are more indolent, estrogen receptor positive, and have a better prognosis [12], and our findings pointed to a similar pattern. Cyclin D1 was statistically significantly correlated with estrogen and progesterone receptor positivity, a lower tumor grade and lower proliferation activity, that is, with breast cancers that have a good prognosis. In addition, high cyclin D1 expression significantly increased breast cancer-specific survival and overall survival in our cohort. Gene amplification of CCND1 has been reported to be associated with an increased risk of breast cancer recurrence, while nuclear expression of cyclin D1 protein was associated with a decreased recurrence rate [23]. Amplification of the CCND1 gene has been identified in approximately 15 to 20% of human breast cancers, while overexpression of cyclin D1 protein has been demonstrated in 50 to 70% [5]. Our data showed a correlation between a high cyclin D1 mRNA level and high cyclin D1 protein expression. Hence, at least some of the increased protein expression of cyclin D1, if not all, must be due to the amplification of the corresponding gene. The prognostic significance of cyclin D1 overexpression with respect to cancer in general does not seem to be consistent, however. High expression of cyclin D1 has been associated with resistance to tamoxifen therapy [24–26]. And in another hormone-dependent cancer type, prostate cancer, overexpression of cyclin D1 is associated with a high proliferative index and a metastatic disease, and it has been suggested that high cyclin D1 may be related to the evolution of an androgen-independent disease form [27].
Although CDK4 was expressed in about 70% of our tumor samples, it surprisingly did not show any significant correlations with the main clinical prognostic factors for breast cancer nor with the survival functions. A tendency for CKD4 positivity to correlate with a higher tumor grade was noticed, however, coinciding with the notion that CDK4 promotes tumor cell proliferation [10]. This is not entirely surprising since others have reported earlier in a similar size German cohort that even though 16% of sporadic breast cancers overexpressed CDK4, no association between any of other clinical factors, except Ki-67, was observed [28]. It is thought that the ability of cyclin D1 to activate CDK4 is critical for driving tumorigenesis, and that CDK4-associated kinase activity is required to maintain this tumorigenesis, as shown in mice with Her2-induced, but not Wnt-1-induced [29] and Ras-induced breast cancer [30]. However, we did not find any correlation between CDK4 and cyclin D1 expression. It has, however, been reported that the oncogenic activity of cyclin D1 in human cancers is independent of CDKs [31]. Not only does the interaction between CDK4/6 and cyclin D1 suggest that they act interdependently, but cyclin D1 has also been reported to function independently of CDK4/6 in supporting proliferation by directly activating estrogen receptors [12]. As noted previously, our data point to a high correlation between positive ER status and cyclin D1 expression, which supports the suggestion of a direct estrogen receptor-mediated mode of function for cyclin D1. Collectively, these data together with some previous data [28] suggest that the role of CDK4 in human breast carcinogenesis differs from that in mouse and further emphasizes the importance of clinical studies.
As a tumor suppressor, p16 is a negative cell cycle regulator, and its inactivation appears to be a common event in many cancers [16], and in many cases it is associated with poorer prognosis. For example, p16 overexpression has been found in high-grade carcinomas of the oropharynx and the genital and genitourinary tracts [32–34]. Yet the role of its overexpression in human breast cancer is a point of controversy. We found a significant correlation between high p16 expression and HER2 negativity, and expression of the oncogene HER2 is considered a very poor prognostic factor for breast cancer [35]. An association of ER negativity, higher grading and high proliferation activity with the overexpression of 16 has been detected in previous breast cancer studies [36], as also has its association with the basal-like phenotype [18, 19]. However, in our cohort, statistically significant correlations between high p16 expression and both increased disease-free survival and increased breast cancer-specific survival were observed.
The expression of p16 has been shown to correlate with the inhibition of VEGF and angiogenesis, but the mechanism by which p16 regulates VEGF has not been properly explored. One hypothesis is that the activity of HIF-1α, which is responsible for hypoxia-induced malignant progression, including VEGF transactivation, can be attenuated by p16 [37]. Moreover, p16 itself has been shown to be hypoxia-inducible [38]. On the other hand, we did not find any correlation between p16 and HIF-1α or HIF-2α, having studied the expression of both of these in the same cohort earlier [21]. The HIF prolyl 4-hydroxylases, PHD1-3, are oxygen sensors that negatively regulate HIF-α [39]. In the present work, p16 had a positive correlation with PHD1 and PHD2, while CDK4 had an inverse correlation with PHD3. The levels of expression of PHD1-3 have been determined in the same set of samples earlier [21], and similarly to high p16 expression, high PHD2 expression had a tendency to promote breast cancer-specific survival and disease-free survival [21]. The most significant correlation found earlier for PHD3 was with a low tumor grade (P = 0.000) [21], while interestingly, there was a tendency for high CDK4 expression to correlate with a high tumor grade, supporting the identified inverse relation between these factors. PHD3 depletion under hypoxia has been shown in a recent study involving a head and neck squamous cell carcinoma line to be associated with cell cycle arrest at the G1/S interface, in that it reduces the amount of hyperphosphorylated RB [40].
To our knowledge, this is the first time when the interrelation between cyclin D1, CDK4 and p16 has been studied in human breast cancer patient samples. We show in this cohort that the expression of two of the studied markers, p16 and cyclin D1, correlates with better prognosis of breast cancer. A significant association of these factors in the rather small cohort with limited number of events highlights the strength of these findings. Our data on cyclin D1 and CDK4 agree with those in some earlier patient studies [12, 14, 28]. The failure to identify an interrelation among cyclin D1, CDK4 and p16 may be due to a limited cohort size, or a more complex interrelation beyond the protein level expressions studied here.
In summary, high p16 expression correlated with HER2 negativity and longer breast cancer-specific and disease-free survival in human breast cancer. In addition, our data confirm earlier findings correlating high cyclin D1 expression with a good prognosis for breast cancer. The expression levels of cyclin D1 protein and mRNA correlated with each other. Surprisingly, no significant correlations among cyclin D1, CDK4 and p16 were found in terms of their expression.
cyclin-dependent kinase
confidence interval
diaminobenzidine tetrahydrochloride
estrogen receptor
hypoxia inducible factor
hazard ratio
phosphate-buffered saline
progesterone receptor
quantitative real time PCR
We thank Tanja Aatsinki, Kari Mononen, Erja Tomperi and Mirja Vahera for their technical assistance.
Authors' Affiliations
- Weinberg RA: The retinoblastoma protein and cell cycle control. Cell. 1995, 81: 323-330. 10.1016/0092-8674(95)90385-2.View ArticlePubMedGoogle Scholar
- Sherr CJ: Cancer cell cycles. Science. 1996, 274: 1672-1677. 10.1126/science.274.5293.1672.View ArticlePubMedGoogle Scholar
- Massagué J: G1 cell-cycle control and cancer. Nature. 2004, 432: 298-306. 10.1038/nature03094.View ArticlePubMedGoogle Scholar
- Tashiro E, Tsuchiya A, Imoto M: Functions of cyclin D2 as an oncogene and regulation of cyclin D1 expression. Cancer Sci. 2007, 98: 629-635. 10.1111/j.1349-7006.2007.00449.x.View ArticlePubMedGoogle Scholar
- Musgrove EA, Caldon CE, Barraclough J, Stone A, Sutherland RL: Cyclin D as a therapeutic target in cancer. Nat Rev Cancer. 2011, 11: 558-572.View ArticlePubMedGoogle Scholar
- Li J, Poi M, Tsai M-D: Regulatory mechanisms of tumor suppressor P16INK4A and their relevance to cancer. Biochemistry. 2011, 50: 5566-5582. 10.1021/bi200642e.View ArticlePubMedPubMed CentralGoogle Scholar
- Witkiewicz AK, Knudsen KE, Dicker AP, Knudsen ES: The meaning of p16ink4a expression in tumors: functional significance, clinical associations and future developments. Cell Cycle. 2011, 10: 2497-2503. 10.4161/cc.10.15.16776.View ArticlePubMedPubMed CentralGoogle Scholar
- Buckley MF, Sweeney KJ, Hamilton JA, Sini RL, Manning DL, Nicholson RI, deFazio A, Watts CK, Musgrove EA, Sutherland RL: Expression and amplification of cyclin genes in human breast cancer. Oncogene. 1993, 8: 2127-2133.PubMedGoogle Scholar
- Paternot S, Bockstaele L, Bisteau X, Kooken H, Coulonval K, Roger PP: Rb inactivation in cell cycle and cancer: the puzzle of highly regulated activating phosphorylation of CDK4 versus constitutively active CDK-activating kinase. Cell Cycle. 2010, 9: 689-699. 10.4161/cc.9.4.10611.View ArticlePubMedGoogle Scholar
- Santamaria D, Barriere C, Cerqueira A, Hunt S, Tardy C, Newton K, Caceres JF, Dubus P, Malumbres M, Barbacid M: Cdk1 is sufficient to drive the mammalian cell cycle. Nature. 2007, 448: 811-815. 10.1038/nature06046.View ArticlePubMedGoogle Scholar
- Thoms HC, Dunlop MG, Stark LA: CDK4 inhibitors and apoptosis. a novel mechanism requiring nucleolar targeting of RelA. Cell Cycle. 2007, 6: 1293-1297. 10.4161/cc.6.11.4312.View ArticlePubMedGoogle Scholar
- Roy PG, Thompson AM: Cyclin D1 and breast cancer. Breast. 2006, 15: 718-727. 10.1016/j.breast.2006.02.005.View ArticlePubMedGoogle Scholar
- Yang WI, Zukerberg LR, Motkura T, Arnold A, Harris NL: Cyclin D1 (Bcl-1, PRAD1) protein expression in low-grade B-cell lymphomas and reactive hyperplasia. Am J Pathol. 1994, 145: 86-96.PubMedPubMed CentralGoogle Scholar
- Boström P, Söderström M, Palokangas T, Vahlberg T, Collan Y, Carpen O, Hirsimäki P: Analysis of cyclins A, B1, D1 and E in breast cancer in relation to tumour grade and other prognostic factors. BMC Res Notes. 2009, 2: 140-147. 10.1186/1756-0500-2-140.View ArticlePubMedPubMed CentralGoogle Scholar
- Lukas J, Parry D, Aagaard L, Mann DJ, Bartkova J, Strauss M, Peters G, Bartek J: Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature. 1995, 375: 503-506. 10.1038/375503a0.View ArticlePubMedGoogle Scholar
- Rocco JW, Sidransky D: p16 (MTS-1/CDKN2/INK4a) in cancer progression. Exp Cell Res. 2001, 264: 42-55. 10.1006/excr.2000.5149.View ArticlePubMedGoogle Scholar
- Chae SW, Sohn JH, Kim D-H, Choi YJ, Park YL, Kim K, Cho YH, Pyo J-S, Kim JH: Overexpressions of Cyclin B1, cdc2, p16 and p53 in human breast cancer: the clinicopathologic correlations and prognostic implications. Yonsei Med J. 2011, 52: 445-453. 10.3349/ymj.2011.52.3.445.View ArticlePubMedPubMed CentralGoogle Scholar
- Bohn OL, Fuertes-Camilo M, Navarro L, Saldivar J, Sanchez-Sosa S: p16INK4a expression in basal-like breast carcinoma. Int J Clin Exp Pathol. 2010, 3: 600-607.PubMedPubMed CentralGoogle Scholar
- Herschkowitz JI, He X, Fan C, Perou CM: The functional loss of the retinoblastoma tumour suppressor is a common event in basal-like and luminal B breast carcinomas. Breast Cancer Res. 2008, 10: R75-10.1186/bcr2142.View ArticlePubMedPubMed CentralGoogle Scholar
- World Health Organization Classification of Tumours Pathology and Genetics of Tumours of the Breast and Female Genital Organs. Edited by: Tavassoli FA, Devilee P. 2003, Lyon: IARC PressGoogle Scholar
- Peurala E, Karppinen P, Haapasaari KM, Bloigu R, Jukkola-Vuorinen A: Expressions of individual PHDs associate with good prognostic factors and increased proliferation in breast cancer patients. Breast Cancer Res Treat. 2011, 133: 179-188.View ArticlePubMedGoogle Scholar
- Isola J, Tanner M, Forsyth A, Cooke TG, Watters AD, Bartlett JM: Interlaboratory comparison of HER-2 oncogene amplification as detected by chromogenic and fluorescence in situ hybridization. Clin Cancer Res. 2004, 10: 4793-4798. 10.1158/1078-0432.CCR-0428-03.View ArticlePubMedGoogle Scholar
- Lundgren K, Brown M, Pineda S, Cuzick J, Salter J, Zabaglo L, Howell A, Dowsett M, Landberg G: Effects of cyclin D1 gene amplification and protein expression on time to recurrence in postmenopausal breast cancer patients treated with anastrozole or tamoxifen: a TransATAC study. Breast Cancer Res. 2012, 14: R57-View ArticlePubMedPubMed CentralGoogle Scholar
- Jirström K, Stendahl M, Rydén L, Kronblad A, Bendahl PO, Stål O, Landberg G: Adverse effect of adjuvant tamoxifen in premenopausal breast cancer with cyclin D1 gene amplification. Cancer Res. 2005, 65: 8009-8016.PubMedGoogle Scholar
- Rudas M, Lehnert M, Huynh A, Jakesz R, Singer C, Lax S, Schippinger W, Dietze O, Greil R, Stiglbauer W, Kwasny W, Grill R, Stierer M, Gnant MF, Filipits M, Austrian Breast and Colorectal Cancer Study Group: Cyclin D1 expression in breast cancer patients receiving adjuvant tamoxifen-based therapy. Clin Cancer Res. 2008, 14: 1767-1774. 10.1158/1078-0432.CCR-07-4122.View ArticlePubMedGoogle Scholar
- Drobnjak M, Osman I, Scher HI, Fazzari M, Cordon-Cardo C: Overexpression of cyclin D1 Is associated with metastatic prostate cancer to bone. Clin Cancer Res. 2000, 6: 1891-1895.PubMedGoogle Scholar
- Lanzino M, Sisci D, Morelli C, Garofalo C, Catalano S, Casaburi I, Capparelli C, Giordano C, Giordano F, Maggiolini M, Andò S: Inhibition of cyclin D1 expression by androgen receptor in breast cancer cells--identification of a novel androgen response element. Nucleic Acids Res. 2010, 38: 5351-5365. 10.1093/nar/gkq278.View ArticlePubMedPubMed CentralGoogle Scholar
- An HX, Beckmann MW, Reifenberger G, Bender HG, Niederacher D: Gene amplification and overexpression of Cdk4 in sporadic breast carcinomas is associated with high tumor cell proliferation. Am J Pathol. 1999, 154: 113-118. 10.1016/S0002-9440(10)65257-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Reddy HK, Mettus RV, Rane SG, Grana X, Litvin J, Reddy EP: Cyclin-dependent kinase 4 expression is essentiel for neu-induced breast tumorgenesis. Cancer Res. 2005, 65: 10174-10178. 10.1158/0008-5472.CAN-05-2639.View ArticlePubMedGoogle Scholar
- Reddy HK, Graña X, Dhanasekaran DN, Litvin J, Reddy EP: Requirement of Cdk4 for v-Ha-ras-Induced breast tumorigenesis and activation of the v-ras-Induced senescence program by the R24C mutation. Gene Cancer. 2010, 1: 69-80. 10.1177/1947601909358105.View ArticleGoogle Scholar
- Lamb J, Ramaswamy S, Ford HL, Contreras B, Martinez RV, Kittrell FS, Zahnow CA, Patterson N, Golub TR, Ewen ME: A mechanism of cyclin D1 action encoded in the patterns of gene expression in human cancer. Cell. 2003, 114: 323-334. 10.1016/S0092-8674(03)00570-1.View ArticlePubMedGoogle Scholar
- Pinto AP, Degen M, Villa LL, Cibas ES: Immunomarkers in gynecologic cytology: the search for the ideal 'biomolecular Papanicolaou test'. Acta Cytol. 2012, 56: 109-121. 10.1159/000335065.View ArticlePubMedGoogle Scholar
- Guihard S, Jung AC, Noël G: High-risk human papilloma virus associated oropharynx squamous cell carcinomas: clinical, biological implications and therapeutical perspectives. Cancer Radiother. 2012, 16: 34-43. 10.1016/j.canrad.2011.11.001.View ArticlePubMedGoogle Scholar
- Yin M, Bastacky S, Parwani AV, McHale T, Dhir R: p16ink4 immunoreactivity is a reliable marker for urothelial carcinoma in situ. Hum Pathol. 2008, 39: 527-535. 10.1016/j.humpath.2007.08.005.View ArticlePubMedGoogle Scholar
- Shah S, Chen B: Testing for HER2 in breast cancer: a continuing evolution. Patholog Res Int. 2011, 2011: 903202-Google Scholar
- Milde-Langosch K, Bamberger A-M, Rieck G, Kelp B, Löning T: Overexpression of the p16 cell cycle inhibitor in breast cancer is associated with a more malignant phenotype. Breast Cancer Res Treat. 2001, 67: 61-70. 10.1023/A:1010623308275.View ArticlePubMedGoogle Scholar
- Zhang J, Lu A, Li L, Yue J, Lu Y: p16 modulates VEGF expression via its interaction with HIF-1alpha in breast cancer cells. Cancer Invest. 2010, 28: 588-597. 10.3109/07357900903286941.View ArticlePubMedPubMed CentralGoogle Scholar
- Zygmunt A, Tedesco VC, Udho E, Krucher NA: Hypoxia stimulates p16 expression and association with cdk4. Exp Cell Res. 2002, 278: 53-60. 10.1006/excr.2002.5564.View ArticlePubMedGoogle Scholar
- Kaelin WG, Ratcliffe PJ: Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol Cell. 2008, 30: 393-402. 10.1016/j.molcel.2008.04.009.View ArticlePubMedGoogle Scholar
- Högel H, Rantanen K, Jokilehto T, Grenman R, Jaakkola PM: Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells. PLoS One. 2011, 6: e27112-10.1371/journal.pone.0027112.View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Fourteen years have passed since the first prisoner arrived in Guantánamo Bay. Almost 800 men have passed through Guantánamo's cells. Today, fewer than 80 men remain. Originally fashioned as an "island outside the law" where terrorism suspects could be detained without process and interrogated without restraint, Guantánamo has been a catastrophic failure on every front. It is long past time for this shameful episode in American history to be brought to a close.
On his first day in office, President Obama promised to close the prison at Guantánamo within a year. With the clock ticking down in his second term, the president's legacy is on the line. Every branch of our government shares responsibility for Guantánamo's ongoing damage to the human rights of detainees, our nation's reputation, and national security. But President Obama and his agencies have the power to end indefinite detention and close Guantánamo the right way.


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The Local Taphouse, St Kilda, is one of Australia's most awarded craft beer venues. We tap over 400 beers a year, dozens more in bottles and get experimental with an infuser. It's not all about the beer though; we love great food and the menu is designed for everyone at the table.


text: No recent wiki edits to this page. Jarail was a Human Sorcerer who fell in love with a female Sorceress named Ozama. Ozama was good hearted, but quick to anger, and always lusting after powerful magic artifacts and scrolls.Ozama's quest for magic led her to Myth Drannor, the ruined undergound city of the Mythral Weave. She bid Jarail to join her, and smitten with love, he could not say no, despite the danger. While exploring Myth Drannor, they got into a heated lovers quarrel. Ozama lost her temper, and in a moment of anger wove a spell that encased Jarial in a magic stone from the waste down. Jarial begged and pleaded to be freed but she told him a riddle instead, and said that once he figured out the answer to the riddle he would be free and could be with her again. Days turned to weeks, weeks to months, and months to years, and Jarail couldn't answer the riddle, or escape the stone prison. His mind went almost mad, in part because of the solitude, but also out of fear for the safety of Ozama. He was certain she was going to return for him after her anger subsided. But she had never came.The nature of Ozama's spell had made Jarial immortal while trapped in the stone, and he did not age, nor need food or water. After over a hundred years past, Jarial was freed of his prison by a band of heros from New Phain, on a quest to put a stop to the Pool of Radiance that had emerged. They were able to solve Jarial's imprisoning riddle and he joined thier party, in hopes to find the fate of his true love Ozama.Ozama's body was found not far from the room Jarial had been trapped in, she had been killed by orcs shortly after leaving Jarial. She was identifed by the magic boots she wore, still on her skeletal feet.


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Dracula's Coming Out - Bran Castle
... philosophers and artists tackled the issue ever more often. Bram Stoker's novel came as the pinnacle of a long series of works based on tales coming from the East. Back then, most readers were certain that the novel had been inspired by real facts and that its story was perhaps just a bit romanticized…
Vlad Tepes adopted the method of impaling criminals and enemies and raising them aloft in the town square for all to see. Almost any crime, ...
A man gave me flowers!
... Instead of going to the actual castle, Raluca, Don, Stella, Heidi and I eat cake and coffee at the cafe there. It's expensive for Romania but nice. Then Raluca and I go shopping but don't end up buying anything.
After that we go to Casa Nostra(Our house or home that would translate to) and I get to try sarmale. It's sour cabbage stuffed with pork and it is amazing-if I haven't covered it already, I LOVE Romanian food. I could eat it all day ...
A Short Stop for Fresh Air
... As King Carol I ruled Romania - from 1866-1914 - he spent much of his time her in Sinaia and had this castle built here. Being from Saxon origins himself, the castle featured styles similar to many of the castles that were built in Germany around the same time. Unfortunately we could not go inside the castle, as it was closed. Simply ...
The Roman Ruins of Jidova -Oct. 20 Part 3
... however, that life experiences, even the ones we do not remember, have a huge impact on how we grow up, and how we view the world.
For example, my mother was a "stay at home Mom" long before the expression was coined. Because of that, and the fact that we had four children in our family, we did not have a lot of disposable income. I also grew up in a time when going to McDonald's was not a common event, but a rare outing. We did not have all of the different ethnic foods ...
Make a Date with Dracula
... br> In 2006, in accordance with the new 2005 law allowing restitution claims on properties seized by the communist government, Prince Dominic of Tuscany, the son and heir of Princess Ileana was awarded ownership of Bran Castle. The Prince promptly put the castle up for sale for a whopping $78 million claiming he would only sell it to a buyer "who will treat the property and ...


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Informationen und Chancen für junge Menschen in ganz Europa.
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Greta Van Sustern's 2002 career move came on the heels of a flurry of cable news talent changes, including those of Paula Zahn (she jumped from FoxNews to CNN) and Geraldo Rivera (he went from NBC to FoxNews). When Van Susteren revealed that she had undergone blepharoplasty (an "eyelift"), she became the subject of headline news and gossip columns… Greta Van Susteren's 2003 book was titled My Turn at the Bully Pulpit… She has stated that Senator Joe McCarthy, a friend of her father's, died when she was "2 years and 10 months" old, confirming her birth year as 1954.
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Today was a great day! Started off rainy but then became the most lovely Uganda day! While I was trying to heat up some water I learned we were out of gas for our stove. We really need to get more gas but it was raining. If you have not heard about life in Uganda and rain let me fill you in…..
When it rains out here life comes to a stop! Taxis don't run, bodas find a place to wait it out, and people just wait. Wait for it to stop raining. I was blessed to wait it out in my flat! While waiting it out my neighbor invited me over for coffee (if you have not caught on us missionaries drink a lot of coffee and tea!). We had a great time chatting. It was really our first opportunity to get to know each other. Yes, I have been living here for almost two months but we are both busy ladies! After enjoy morning together I decided to try to get some gas….after all I did want to be able to cook something at some point today.
So, I set of to town with my friend and boda driver Emma. He was great. Got me in and round town EVEN with Starday traffic! I got to the store (not the gas store the other store) and I got a few things and then got really inspired! lets just say my living room has a new coat of paint! Well, almost all of it does. I am a little to short to reach up top so I will have to work that our some how. But wait I am not writing about paint! Or gas for the stove! It is about the film Narnia!
You, that is right the great movie that was first a book by C.S. Lewis! While painting my living-room I put in the movie because some how we had power! YES POWER (sorry but I get excited when we have power for more then 12 hours at a time!)!!!! Any way. As I was watching and painting I really saw the story of Christ in it. I know I know C.S. Lewis is a Christan and wrote it in a way that shows the story of Christ. Something this time really made me see it AND feel it. Maye it is living in a place that is so in slaved to darkness. Or the fact that everyone out here believes in the spiritual world but yet still not does know the one true God.
Lets just say I had a thought…while watching and painting ( am good a doing many things at the same time). What would happen if missionaries or any Christian would start showing Narnia instead of the Jesus film? Please don't take it the wrong way…the Jesus film is powerful and has changed MANY lives. But, what is people saw the story of Christ in a way that moved them, made them want to watch, and for those who are more "closed minded" didn't even know it was the story of Christ. People today are so passive. Whatever works for you is ok as along as whatever works for me is ok with you. COME ON PEOPLE! That way of thinking and living is only more reason to start showing Narnia!
So, I am just saying Narnia or Jesus Film?


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Order Tracking
This project received 40 bids from talented freelancers with an average bid price of $1333 USD.Get free quotes for a project like this
Project Budget$750 - $1500 USD
Total Bids40
Project Description
Need a small order tracker for the company's customer.
The customer is assigned an userid and password iinially.
The customer logs in the appln to retrieve and display list of orders with their Due dt and some notes.
This can be on a mysql db.
Data will be populated from an ERP once every 2 hrs .. to refresh in MYSQL DB..
Provision for .. Forget PW routine..
Activity log maintain / report
Skills Required
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- 1 a person whose job is to keep the records or accounts in an office, shop/store etc. an office clerk see also filing clerk See related entries: Job titles
- 2 an official in charge of the records of a council, court, etc. the Town Clerk the Clerk of the Court see also clerk of works, county clerk, parish clerk
- 3 (North American English) = sales clerk The clerk at the counter gave me too little change.
- 4(also desk clerk) (both North American English) a person whose job is dealing with people arriving at or leaving a hotel synonym receptionist See related entries: Hotel people Word Origin Old English cleric, clerc (in the sense 'ordained minister, literate person'), from ecclesiastical Latin clericus
nounjump to other results
BrE BrE//klɑːk//; NAmE NAmE//klɜːrk//Hotel people, Job titles
Check pronunciation: clerk


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We use a one-on-one process to analyze your unique situation and offer products of lasting value to provide you with long-term strategies to your needs.
IMG Financial Group - Proud donors to the Folds of Honor Foundation, which provides scholarships to the spouses and children of soldiers killed or disabled in service to America.
Service that excels is our trademark. We provide consistent, responsive service thats underscored by the dedication of our staff and our commitment to provide strategies that reflect the best interests of our clients.
At IMG Financial Group, Inc. we offer a comprehensive line of products and services, as well as diverse financial expertise and responsive service. From insurance needs to college funding, retirement or estate planning, we're committed to helping you accomplish your long-term financial objectives. Our corporate values of Trust, Integrity, Respect, and Quality define who we are as an organization since 1981.
We provide objective customized solutions to assist individuals, professionals and business owners in reaching their financial goals. >> Read More
Come join IMG Financial Group and Amcap's Annual Charity Golf Tournament benefiting the Folds of Honor Foundation on November 14th at Braeburn Country Club!
There will be fantastic hole in one prizes along with long drive and closest to the hole!
The tournament will be followed by an awards dinner, raffle, and silent auction. Please click here to for more information about The Folds of Honor Foundation and for general information about the tournament.
Investment Strategies
Retirement Strategies
Estate Planning


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HP, Lenovo, Dell Unite in $70 Million PC Ad Campaign
The OEMs, Intel and Microsoft look to revive PC sales by highlighting what new systems with Windows 10 and Skylake chips can do.The world's top three PC makers are joining with Intel and Microsoft in a six-week, $70 million advertising campaign designed to boost sales in what has been a declining market over the past three-plus years. In an unusual demonstration of solidarity that highlights the frustration they've felt as worldwide PC shipments have fallen since 2011 and the confidence they have in the new systems coming to market, Lenovo, Hewlett-Packard and Dell are working together on the campaign with the tagline "PC Does What?" The joint campaign—which will hit television, digital and social outlets as well as native advertising when it launches in the United States and China Oct. 19—is aimed at highlighting the new form factors and features in new and upcoming systems that will be powered by Intel's latest 6th generation Core "Skylake" processors and run Microsoft's new Windows 10 operating system. The combination of the new OS, processors and system form factors coming at the same time is unique, according to Steven Fund, chief marketing officer for Intel. "It's a time unlike any other in our industry," Fund said Oct. 15 during a streamed panel discussion that included his counterparts at the other four vendors.
The decline in PC shipments worldwide has been blamed primarily on the rise of mobile devices like smartphones and tablets, as consumers and business users redirected their technology dollars away from PCs and lengthened the lifespan of their systems. At the same time, PC vendors and component makers were slow to respond to the threat, failing to come out with new form factors and technologies that could entice users to come back.
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Windows 8 for Dummies + DVD
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About this item
This book and DVD bundle offers a two-pronged attack for helping new and inexperienced Windows users get a grip on the Windows 8 operating system. The book walks readers through the basics, from the core activities that don't change from version to version, to the tools that enhance the system, to the latest updates that make Windows 8 different from previous versions. The four-hour DVD offers short, two- to five-minute walkthrough video lessons on the most common Windows 8 tools.Bundle includes a copy of "Windows 8 For Dummies, " by bestselling For Dummies author Andy Rathbone, and four hours of video training on DVDOffers the core components of the desktop, navigating the dual interfaces, and managing the file and folder systemIncludes the basics for connecting to the Internet, e-mail options and operation, working with the Internet Explorer browser, setting virus protection, and other security toolsShows how to customize and upgrade Windows and add music, movies, and photosAddresses common problems and messages, switching to a new Windows 8 computer, and finding more help
This book and DVD bundle makes a winning combination for any Windows 8 user, no matter what experience level.


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Bessboro Builders, Inc.
(518) 962-4500, 252 Rte 22, PO Box 373 Westport, NY
www.bessborobuilders.com E-mail
New construction, restoration, remodeling, plumbing/heating, electrical, Retail hardware and lumber.
Bessboro Gifts & Clothing Boutique
(518) 962-8281, 6472 Main St. Rte 9N, 22 PO Box 426 Westport, NY
Gift shop featuring Vera Bradley handbags & accessories, jewelry, local pottery, candles, linens, stationery. Clothing boutique features Alps, Tribal, Splendor & Woolrich sportswear for men and women. Laundry, dry cleaning pick up; Gift wrap and mail, UPS.
Champlain National Bank – Westport
(518) 983-3316, 6478 Main St, PO Box 99, Westport, NY 12993
www.champlainbank.com – E-mail
Full service independent, local, community-owned commercial bank.
Champlain Valley Milling
(518) 962-4711, 6679 Main St. Rte 9N, Westport, NY 12993
Producers of full range of organic flours.
Chazy Westport Communications
(518) 962-8211, 2 Champlain Ave PO Box 249 Westport, NY 12993
Keeping YOU Connected since 1905… In addition to quality telephone service and unlimited nationwide calling we offer FIBER optic Broadband with speeds from 14 Mbps to 100 Mbps.
Crane Mountain Valley Horse Rescue
(518) 962-8512, 7556 NYS Rte. 9N Westport, NY 12993
Dedicated to equine rescue and rehabilitation, and to restoring horsemanship, the heritage and humane treatment of the horse. E-mail
Creative Paint
(518) 962-2043, 1167 Ledge Hill Rd, Westport, NY 12993
Peter and Carolyn Vanderhoof
Interior and exterior house painting business with 20 years experience. Quality work with attention to detail. Bonded and Insured. Also Ledge Hill Studio.
Hance Heating & Plumbing, LLC
518-429-3037, 780 NYS Route 22, Westport
Heating, Ventilating, and Air Conditioning.
Emmet Carter Green Design
518-907-4581 A full-service interior design firm fusing green design, ecological sustainability and historically sensitive architectural renovation. http://emmetcarter.com
Lakeside School
518-963-7385, 6 Leaning Rd. Essex, NY E-mail
A developing Waldorf School welcoming students from birth through 2nd grade on a working farm. Located at Black Kettle Farm
Law Office of Julie A. Garcia
(518) 546-1040, 4314 Main St, Port Henry, NY 12974
http://www.garcialawyer.net – E-Mail
Legal services, criminal defense, traffic tickets, real estate, wills, family law.
Ledge Hill Studio
(518) 962-2043, 1167 Ledge Hill Rd. Westport, NY 12993
Furniture shop — Art studio.
Meadowmount School of Music
(518) 962-2400, 1424 County Rte 10 Westport, NY 12993 www.meadowmount.com – E-mail A seven week, summer school for talented music students. Students work under outstanding faculty and present three concerts each week, open to the public, Sunday, Wednesday,& Friday 7:30 pm.
Mental Health Association in Essex County
(518) 962-2077, (800) 440-8074, 6096 NYS Route 9N, Westport, NY 12993
Advocates and provides mental health support services. Self Help; Community Centers; Crisis Alternatives: 24 hours, seven days. United Way of Clinton and Essex Counties agency. E-Mail
MLM Insurance Agency
(518) 962-4467, 192 Champlain Ave. Rte 22, PO Box 40, Westport, NY 12993
Wayne Shepard. General insurance, Home, Auto, Business, Boat, RV, Etc.
North Country SPCA
(518) 873-5000, 7700 Route 9N Elizabethtown, NY 12932
www.ncspca.org E-Mail
Essex County's SPCA. Shelter for abused, abandoned & surrendered dogs and cats and adoption center. New facility in 2013. A no-kill shelter that gives each animal individual attention, medical treatment and compassionate daily care until a loving home can be found.
Overtime Photography
(518) 412-2712, 60 Sisco St. PO Box 413, Westport, NY 12993 www.overtimephotography.com Large capacity photo booth entertains guests and creates memories with unlimited photos during events. Fun props, photo frames, a scrapbook, custom DVDs, and green screen options. Personalize photo strips with date, names, logo, etc. Custom layouts available. Serving New York and New England, but will travel anywhere. E-Mail for brochures and questions.
Ship's Store Boutique
(518) 962-4356, 20 Washington St, Westport, NY 12993
www.westportmarina.com – E-mail
Lake Champlain, Adirondack, children's books; toys, clothing, tableware, gifts
gifts, jewelry, marine supplies. Something for everyone! Expect the unexpected.
S.S. Stephens
(518) 962-4948, PO Box 421, Westport NY 12993
Interior design and fine furnishings. E-Mail
Taylor Tree Care & Landscaping
(518) 962-4986, 187 Youngs Rd, Westport, NY 12993 – Ted Taylor
Tree and shrub care, pruning, removals, stump grinding.Fully insured, Certified NYS Arborist – E-mail
Greenhouse & Nursery Landscaping, locally grown trees shrubs; Annuals, perennials.


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"L'appartamento è grande e in un bellissimo quartiere. Ha diversi problemi di incuria però, in una delle due stanze da letto c'è il parquet sollevato di 10 cm per una probabile perdita d'acqua, le fodere delle poltrone sono sporche e l'intonaco è crepato in diverse stanze. La lavastoviglie è rotta e l'anta del frigo a incasso è divelta sia sotto che sopra nel freezer. Abbiamo trovato i vetri della sala molto sporchi e li abbiamo puliti, così abbiamo goduto del panorama su rue Vavin. Nel complesso, nonostante l'aria un tantino fatiscente, siamo stati molto bene."
"The owner was super helpful, explained all the features of the apartment well. Was more than helpful with getting one lost suitcase to us. Only issues...apartment floors need some work, some of the heaters did not work, walls had some peeling paint, grout on shower needs work. But we did love the apartment and would stay again. Fabulous location and quiet."
"Neighborhood is great, quiet with good access to stores, restaurants, etc. The condition of the apartment was very disappointing for 2 reasons: first, there were unrevealed damages to the bedroom floors and a window shade that were not disclosed until arrival, and second, the furniture was stained, and in general the apartment was "tired". Examples include cracks in a wall, wall scuff marks, gunk in the bathroom grout, cracks in laminate. When I contacted the manager, we did receive a 10% discount, but the project was a below-average experience for the dozens of places we've stayed around Europe. "
"Over all, good location. There were a few things that were not according to our expectation. The window shade in one of the bedrooms would not open and there is no AC in the room and it made it extremely hot and uncomfortable. The wood-floor is not even and we couldn't close the door in one room making it uncomfortable and not private. The small park in front attracts teenagers that are very loud at night forcing us to close the windows and since there is no AC it made the room hot. The Internet is very slow but manageable. Other than that this is an ok apartment."
"the location was fantastic and the space was great. The sitting was good but the bathrooms were very poor and should be updated, there was mould on the shower, paint falling off the walls (and also in the bedroom). There were bare light bulbs in places. The tumble dryer didn't work. This place has so much potential but has not been well maintained and just needs a good decorating to fulfil its potential."
"The flat was wonderful! 3 couples stayed and we had plenty of room. Great location, 2 blocks from a metro station. We loved opening up the door onto the Juliet balconies and enjoying the sights and sounds of Paris. We would stay here again in a heartbeat! Thanks for a great stay!"
"The location and space. The contact person, Anya, was very helpful and pleasant. Old food left by previous visitors, some furniture was torn, not enough room to store clothes or bags because there were other things stored presumably by the owner taking up the space. The apartment was reasonably clean, but only on the surface it really needed more thorough cleaning."
"The area around was great for restaurants, groceries and close to underground. A little further from the center than we would have liked. We had to get on metro to go anywhere and would prefer to walk. Only had use of elevator one day as key thing stopped working. We had to haul all cases down 2 flights even though we asked for replacement and knew there was a spare!"
"Just what we wanted, great location, they were there when we arrived, lovely...."
"Buurt was geweldig, alles dichtbij: uitgaansgelegenheden, park, metro etc. Voldoende ruim appartement voor 6, fijne eettafel. Uitzicht appartement. Kookgerei kan beter: weinig pannen, geen broodzaag aanwezig. Wat kleine reparaties nodig: koelkast stuk, hier en daar wat lichten stuk."
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The Korean underwater robot Crabster being deployed from its mothership while assisting rescue operations near the site of the Sewol ferry disaster.
Paramount Pictures Corporation
June 29, 2011 --
In the movie "Transformers: Dark Side of the Moon," Autobots, transforming robots from the planet Cybertron, work alongside humans to battle the Decepticons, a rival group of robots bent on destroying the universe. The latest Transformers installment may just be a work of fiction, but would real-life robots on Earth be up to the task of saving the planet from the threat of an overwhelming destructive force? Of course not, but that doesn't mean they won't get there one day. Until then, we present the next best thing in this slideshow of incredible real-life robots.
Alex Kossett and Nikolaos Papanikolopoulos, U
Rolling Robot
We begin the slideshow with an actual real-life transforming robot. Although it doesn't tranform into a sentient, humanoid machine, this robot can transform from what appears to be an elaborate motorized rolling pin to a helicopter when the terrain starts to get rough.
Take a look at this flying robot in action here. BLOG: Rolling Robot Transforms into a Helicopter
Boston Dynamics
What's more terrifying than a hungry, fully grown cheetah chasing you down? How about if it were made of metal? Meet CHEETAH, a robot designed by Boston Dynamics for one purpose: to hunt you down like an animal. Once built, this robot will be fast, agile and strong enough to chase down, catch and subdue even the fastest human runners. This robot is one of a line of prototypes known as "terror bots." An appropriate name, especially if you see this guy biting at your heels as you run full speed. BLOG: TERROR BOTS BEING DESIGNED TO HUNT YOU DOWN
If this robot reminds you of Scorponok from the "Transformers" film series, you wouldn't be far off. Designed by researchers at the University of Bielefeld, this advanced walking robot is based on a rather simple creature: an insect. HECTOR, short for Hexapod Cognitive autonomously Operating Robot, has six legs and elastic joints that allow its motions to mimic muscle movement. This construction allows the robot to navigate over uneven terrain. At a little more than three feet long and weighing in at 26 pounds, this robot probably won't be involved in any world domination schemes anytime soon. BLOG: HECTOR THE WALKING ROBOT INSPIRED BY INSECTS
Georgia Tech
Snake Robot to the Rescue
Cheetahs and insects aren't the only animals inspiring robotics' engineers. Mechanical snakes are also being designed to mimic their mechanical counterparts. Unlike CHEETAH, which is made to hunt you down, this snake robot, created by researchers at Georgia Tech Univeristy, is actually designed to come to the rescue. Their unique body shape allows them to burrow through uneven soil. With this unique feature, emergency responders could deploy these robots after a particularly devastating natural disaster, such as an earthquake, when victims are buried and out of reach. BLOG: SNAKE-LIKE ROBOT SWIMS TO THE RESCUE
Running Robot
Robots may not yet be able to conquer the Earth, but what about the wide world of sports? They're already playing soccer and tossing baseballs. Now it looks like they're competing in marathons. (Well, robot marathons anyway.) Last February, five bipedal robots ran a non-stop 26.2-mile race on a 100-meter indoor track in Osaka, Japan. But don't expect these machines to compete with humans anytime soon. Robovie-PC, the winner of the race, finished in just under 55 hours. BIG PIC: TOY-SIZED HUMANOID WINS ROBOT MARATHAN
Ingmar Posner, Oxford Mobile Robotics Group
Reading Robot
No this robot can't run or jump or slither or swim. So what can it do? This machine, known as Marge, has a very different ability entirely: It can read -- and it can learn. Marge may just look like a Tonka truck underneath a coffee pot, but this machine is actually smart enough to read The New York Times and BBC Online. It is even a skilled editor and can identify and correct misspellings. And because Marge's brains are built in its software, not its hardware, this same programming could make its way into other devices, such as cell phones. BLOG: ROBOT CAN READ, LEARN LIKE A HUMAN
Bret Eckhardt
Ethical Robot
Can anyone really tell the difference between right and wrong? Well, this robot can. This robot's ethical code is based on a software program modeled on an approach to ethics developed in 1930 by Scottish philosopher David Ross. As a result, this robot is designed to take the moral high ground -- and will tell on you if you're doing something wrong. Sure this robot doesn't have the firepower of an Ironhide or a Starscream, but a judgmental expression and a jittery nod of disapproval can be just as damaging. BLOG: ROBOT MAKES ETHICAL DECISIONS
Robonaut 2
Meet Robonaut 2, a humanoid robot that could one day be your co-worker -- or even take your job. Yes, this robot has everything any employer looks for in a diligent worker: It's capable and tireless, and it doesn't ever need lunch or bathroom breaks. In fact, you'll never guess where this robot is currently employed: the International Space Station. Robonaut 2 is currently working along astronauts, helping with basic maintenance tasks, such as cleaning. DNEWS VIDEO: MEET 'ROBONAUT 2,' YOUR FUTURE CO-WORKER
Robonaut 2 isn't the only robot on the space station with a job. Dextre, the Canadian robot that lives outside the International Space Station, has been tasked with refueling satellites while in low-Earth orbit. The robot will also be capable of performing minor repairs. In other words, this robot is essentially a space mechanic. Although a gas-pumping robot may not seem like much, Dextre could pave the way for an entirely new industry for satellite servicing. NEWS: SPACE STATION ROBOT LANDS A JOB
When Bong-Huan Jun first saw news of South Korea's Sewol ferry sinking with hundreds of high school students trapped on board, he had stopped by a highway service area on his way to work. But unlike many South Koreans helplessly watching the live broadcast on TV, Jun knew he had something that could help out — an experimental underwater robot named Crabster.
Crabster was designed by Jun and his colleagues at the Korea Research Institute of Ship and Ocean Engineering (KRISO) as a huge, six-legged robotcapable of scuttling along the ocean floor. The robot can withstand strong tidal currents and carries both sonar and acoustic cameras capable of seeing through murky underwater conditions — precisely the conditions divers had to struggle with as they searched the Sewol ferry wreck in the cloudy waters of the Yellow Sea near Jindo Island.
Top 10 Space Robot 'Selfies': Photos
But Crabster had only just begun underwater testing in July 2013 and remained relatively untested. Now it faced one of the most challenging tests for any underwater robotic vehicle during one of South Korea's greatest maritime disasters in its history.
"When I knew the rescue team had serious difficulties due to the high current and turbid water at the accident area, I called Dr. Sanghyun Suh, director general of KRISO and talked about Crabster's functions and possibilities for helping with the rescue," Jun said. "The task force team of my institute reviewed the underwater robots made in KRISO and agreed to send Crabster to the area."
5 Major Advances In Robotic Prosthetics
The Sewol ferry sinking on April 16 had already kicked off a frenzy of rescue operations by ships and divers. But South Korean government officials eventually requested the Crabster team's help on April 20 and allowed the team to move the robot from Namyangju to Jindo Island on April 21.
When Jun and the Crabster team arrived aboard a mothership, they found dozens of ships and a swarm of smaller boats surrounding the main rescue barge anchored near the ferry sinking site. Divers were working with a visibility of less than 20 centimeters at a depth of about 45 meters below the surface. They also had to deal with maximum tidal currents of more than 15 kilometers per hour. (Crabster experienced currents of less than 5 kilometers per hour during its initial deployment.)
"Crabster can stay deeper and longer, and it can see father," Jun explained. "But Crabster cannot go into the ship. We wanted to work together with human divers, but we had no chance to do."
The robot successfully used its acoustic camera and sonar to capture images of the seafloor, providing a stable platform for surveying the ferry wreck despite the tidal currents.KRISO
The South Korean Coast Guard refused to allow the relatively untested Crabster to work directly with human divers at the ferry wreck, but it eventually gave the robot's team one and a half hours to survey the sunken ferry from a distance of 70 meters. Jun and his colleagues had originally wanted permission to tie their ship to the large rescue barge that was firmly moored to the seafloor with four anchors, so that they could steady their smaller ship against the wind and waves while deploying Crabster. But they made the best of their circumstances during the 20 days they spent at Jindo Island.
Strong waves and wind created a rolling motion in the mothership that made it difficult to launch or retrieve the 700-kilogram Crabster during bad weather days. Such bad weather meant the team spent 15 of their 20 days of deployment stuck in port waiting for conditions to improve.
Top 10 Space Robot 'Selfies': Photos
During the initial survey, the mothership drifted too close to the rescue barge and required the help of a nearby tugboat to back out again. A lack of space aboard the mothership also meant the team went without sleep for the first three days and two nights, until they retired to the nearby Pengmok Harbor to rest and wait for additional windows of opportunity.
Still, Jun and his colleagues ended up launching the robot 13 times, allowing the robot to spend 15 hours and 36 minutes in the water. After an initial two-day survey, the team spent an additional three days surveying the sunken Sewol ferry from distances of 500 meters to 1 kilometer.
Despite its baptism by fire, the Crabster robot worked perfectly as it walked across the seabed containing mud, stones, and small pieces of shell. The robot successfully used its acoustic camera and sonar to capture images of the seafloor, providing a stable platform for surveying the ferry wreck despite the tidal currents.
5 Major Advances In Robotic Prosthetics
The Crabster team eventually returned to their home base in Namyangju on May 20 and unloaded the robot. There they joined their fellow citizens in confronting the grim death toll from the Sewol ferry disaster — at least 286 dead, most of them students on a school trip. South Korean president Park Geun-hye ordered the dismantling and reorganization of the country's coast guard on May 19 as part of several reforms in the wake of the tragedy, according to BBC News.
Jun believes that Crabster needs additional experience in the water before the South Korean government will fully trust the robot's capabilities — he had expected the robot to be used even more than what the coast guard had allowed. But he and his colleagues hope to build upon their experience to be better prepared for any future rescue missions that may come up.
"I think the next Crabster should be a powerful hydraulic-powered one having enough power to break the doors and windows of cabins," Jun said. "Then the robot will be able to install guide ropes and make routes to inside the ferry for divers at the initial rescue stage."
Get more from IEEE Spectrum
Robots Playing Ping Pong: What's Real, and What's Not?
nmanned Cargo Ships Face Industry Resistance, Are a Good Idea Anyway
Meet the Robots of Fukushima Daiichi
This article originally appeared on IEEE Spectrum; all rights reserved.


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Voted Rising Star on the Trumpet five years in a row by DownBeat Magazine, Jeremy Pelt performs works from his new CD Men of Honor. "Mr. Pelt plays brilliantly, with warmth and depth" (The New York Times).
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Virzì at the Oscars
There were nine other titles in race. So 2010 seems to be confirmed as a golden year for the Italian director
A choice that everybody agrees on because, with a good movie, it brings back one of the most important genres in our cinematography, loved in our country and in other countries: the Italian comedy.
There were nine other titles in race: the magnificent L'uomo che verrà by Giorgio Diritti, La nostra vita by Daniele Luchetti, La doppia ora by Giuseppe Capotondi, Io sono l'amore by Luca Guadagnino, Baciami ancora by Gabriele Muccino, Mine vaganti by Ferzan Ozpetek, 20 sigarette by Aureliano Amadei and the very interesting Basilicata coast to coast by Rocco Papaleo and Le quattro volte by Michelangelo Frammartino, both, either way, too out of line to take part in the competition for the Academy Award.
And so 2010 seems to be confirmed as a golden year for Paolo Virzì: the critical and public success for the movie that was released in January, the birth of his son Jacopo from his wife Micaela Ramazzotti in March, the silver ribbons in July (one for directing, one for his wife as best leading actress with Stefania Sandrelli and one for the screenplay), and the good sales of the movie in different countries.
La prima cosa bella, now released in dvd, describes the figure of a woman (portrayed, as we were saying, by Ramazzotti and Sandrelli) too beautiful for her exuberance and simplicity, through the memories and through the present of her son (Valerio Mastandrea) that finds her years later while she is dying.
Paolo Virzì and Micaela Ramazzotti
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Sasquatchfabrix. offers it's take on the Vietnam-era combat jacket. Signifying their anti-war outlook, the jacket is adorned with rear 'Do Not Kill' embroidery in Japanese characters. The front of the jacket features two large patch pockets with vertical entry. Rounding out the details is a rough finished hem, metal button closure and button cuffs. Made in Japan from a durable cotton twill fabric.
Model is 6'0" (182cm) tall, weighs 150lbs (68kg) and is wearing a size L
HAVEN will not accept any returned merchandise without prior written communication and valid RETURN AUTHORIZATION NUMBER. All items that may be authorised to be returned to HAVEN must be in 'brand new, unworn condition' and can be done so with proof of purchase within 14 days from purchase date and within 7 days for in store purchases. An instore credit will be provided, NO REFUNDS whatsoever. All sale items and discounted merchandise are FINAL SALES, no exceptions. Read More


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Crackers and Hackers
The aboriginal absorbing affair to apperceive is that there is a aberration amid a hacker and a cracker. A hacker tries to analysis a arrangement for accessible aegis and arrangement breaches, tries to apprentice as abundant as accessible from a arrangement and tries all strategies to analysis the arrangement for its optimum achievement level. That is all absolute and no awful intent. Whereas a cracker tries to bypass system's aegis checks for accomplishing his angry assets such as:
- Stealing abstracts about the registered users
- Getting authority of acclaim agenda numbers and passwords for accessible reasons
- Financial or business information
- Some added advice for accouterment abeyant accident to the buyer of the system
- They can get passwords with usernames and claimed pictures and photographs of ancestors and baby ones
- Our computer can aswell be acclimated for Denial of account attacks on added PC's
- Crackers can again advertise on this baseborn advice to online spammers. This can eventually advance to an alarming access in the cartage to some organizations' website for accessible blast down of its server. The PC can aswell be acclimated as spam bot
There is so abundant that a cracker can do that there is not limit. One affair for abiding is: every arrangement that goes online needs to be adequate adjoin such attacks. The bigger the website or organization, the added accident it is at.
Why do Passwords Leak
If you are into arena computer games and visiting online gaming websites, you should be acquainted of the abeyant arise threats that your arrangement is decumbent to. Gamers accept the addiction to actualize accounts on abounding websites' forums and account boards to get abreast of the latest updates and cheats etc. When they actualize their accounts on abounding websites, they leave their usernames and passwords with them. The added advice you accept on internet, the added at blackmail you are. Moreover, bodies are advised to be absorbed appear simple and simple paths so we accept passwords that are simple to bethink and this makes them abundant easier for the absurd to crack.
Various methods can be acclimated to able passwords like the animal force advance area a arrangement is bombarded with frequently acclimated words from a concordance and this is area simple to bethink passwords can be absolutely accessible for the crackers. Downloading bold mod is aswell a abeyant aegis threat. Such software can aswell accommodate bacilli that are downloaded with them and achieve in the PC, hidden from your ability and knowledge. Such bacilli can aswell save keystrokes and appropriately your passwords and usernames or any such advice can be leaked.