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Since living in Shanghai I have seen Chick Corea, Beyoncé, Björk, and Harry Connick, Jr. None of those artists are exactly the sort of people I normally see in concert. In fact, likely as not were I still living in the States I would not have shelled out good money to see any of them. For some reason though, China makes me rush to see everybody whose name I at least know.
Except Celine Dion. Screw that.
I paid more money than I've ever paid to see anybody to see Björk, and I barely know any of her music. I'm actually more familiar with her work in the Sugarcubes than as a solo artist. I know her songs from those crazy videos she released in the early 90s, and I have a copy of her recent release Medúlla, but while I find that disk strangely beautiful, I've not listened enough to really know the songs.
Björk played Shanghai at the International Gymnastic Gymnasium which remains an active gym complete with bad lights, hardwood floors, and a section of tables for the judges. In the off season they have erected a stage in one corner, and thrown down some folding chairs on the floor. As such neither the view or the sound quality is particularly great for a concert.
Björk hit the stage a few minutes behind schedule but with such gusto that I forgave her immediately. Her band consists of a ten piece horn section, a keyboardist, a percussionists and some guy on a lap top. It was the horns that came out first blasting a lively, booty shaking romp before the gut obliterating percussion came rolling out. What the room lacked in refined acoustics, the band made up for in sheer volume and reverberations. The beats vibrated my insides and shook the seats. It made me itch. Literally. The vibrations made my skin crawl and between dance moves I had to lay down some scratching of my own.
Björk gyrated about the stage with frantic energy and wailed with a voice that is a cross between sweet soul music and an angry grizzly bear. It was fantastic! I know some of her songs are in English and I believe she said a few words in my native tongue, but honestly the only word I understood all night was the Chinese word for "thank you." It didn't matter a lick though, as the music and the sounds emanating from her voice was beautiful, even if I didn't actually understand it.
Björk seems to be just as well known for her peculiar wardrobe as her music, but she was somewhat subdued this night. Dressed in a brightly colored smock with what appeared to be tight leather leggings, she didn't look any weirder than some of the girls I see walking about the city (well except for the face paint.) The Icelandic horn section were also decked out in bright outfits complete with poles and flags attached to their backs. The keyboardist was strangest of all as he was fitly dressed in a nice suit and tie which did nothing but stick out amongst all the color.
Though I didn't recognize but one or two songs they had me shaking in my seat from song one. In fact the seat was no longer needed after about song three as nearly the entire audience got to their feet and shook it for the rest of the show. The kinetic power of the audience mixed with the strobe like effects of the lights made me feel more like I was out clubbing at some techno joint rather than at a concert. Or what I imagine going clubbing at a techno joint would be like, as the closest I've ever gotten to one is the cineplex.
There has been a great deal of controversy over the encore, "Declare Independence." It is a song of personal independence and during the chorus Bjork found herself chanting "Tibet, Tibet" several times. She has been banned from the country for that performance and there has been much talk over what effect this will have on future pop acts in China.
It has only been the last few years that the government has been allowing in popular musical groups and it is such outbursts that I suspect have kept them so timid for so long.
I'd like to stand proud having been there at such a big moment. I'd like to describe in detail what happened when when she chanted for liberation. How the crowd got to their feet in support and the powers that be tried to squash the Icelander immediately, but I simply can't.
Honestly, I thought the lyrics were "erase your flag" and neither myself nor anybody I know had any idea she mentioned Tibet until days later when the controversy started. From memory the reaction of the crowd was nothing more than enjoying the encore. By that point in the show pretty much everybody was on their feet, shouting, and dancing. There weren't any boos, or noticeable reactions to the song or her chant. Things went on as normal, and I hadn't the slightest idea I had just seen something controversial.
Though she won't be back to China anytime soon, and despite the bad acoustics and controversy, Björk put on a wild, rollicking show. I highly recommend her to everyone.
Here's a clip of the controversial moment:
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|Named Person:
|Rachel Carson; Rachel Carson
|Material Type:
|Document Type:
|All Authors / Contributors:
Jim Lynch
|1582346054 9781582346052
|OCLC Number:
|247 pages ; 22 cm
|Jim Lynch.
|More information:
A mesmerizing, allegorical, and beautifully wrought first novel about one boy's fascination with the sea during the summer that will change his life. One moonlit night, thirteen-year-old Miles O'Malley slips out of his house, packs up his kayak and goes exploring on the flats of Puget Sound. But what begins as an ordinary hunt for starfish, snails, and clams is soon transformed by an astonishing sight: a beached giant squid. As the first person to ever see a giant squid alive, the speed-reading Rachel Carson-obsessed insomniac instantly becomes a local curiosity. When he later finds a rare deepwater fish in the tidal waters by his home, and saves a dog from drowning, he is hailed as a prophet. The media hovers and everyone wants to hear what Miles has to say. But Miles is really just a teenager on the verge of growing up, infatuated with the girl next door, worried that his bickering parents will divorce, and fearful that everything, even the bay he loves, is shifting away from him. While the sea continues to offer up discoveries from its mysterious depths, Miles struggles to deal with the difficulties that attend the equally mysterious process of growing up. In this mesmerizing, beautifully wrought first novel, we witness the dramatic sea change for both Miles and the coastline that he adores over the course of a summer - one that will culminate with the highest tide in fifty years.
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Washington Reads (Winter 2008) Selection
by WashingtonStateLibrary (WorldCat user published 2008-03-10) Permalink
Olympia author Jim Lynch's masterful first novel intertwines the untold stories of the sea with many of life's mysteries. Lynch's knowledge and sense of wonder with the natural world shine through this book. Many Olympia and Mud Bay locations are named, while other names are fictitious. This coming of...
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The molecular events leading from cardiomyocyte ischaemia to inflammatory cytokine production are not well understood. We previously found that heat shock protein 60 (HSP60) appeared in extracellular space after cardiomyocyte ischaemia. This study examined the activation and regulation of toll-like receptors (TLRs) by HSP60 in cardiomyocytes.
Cytokine production and TLRs regulation mediated by TLRs signalling were examined in response to exogenous HSP60 (exHSP60) and endogenous HSP60 (enHSP60) released extracellularly under ischaemia. The results showed that exHSP60 induced inflammatory cytokine production in adult rat cardiomyocytes and H9c2 cells (a standard cardiac cell line derived from embryonic cells), through a pathway dependent on TLR4, myeloid differentiation factor 88 (MyD88), p38, and nuclear factor-κB (NF-κB). Further study revealed up-regulated expression of both TLR2 and TLR4 by exHSP60, which was dependent on the activation of TLR4, MyD88, c-Jun NH2-terminal kinase (JNK), and NF-κB, but not on p38. In myocytes exposed to ischaemia, enHSP60 was released into the media, and triggered cytokine production and TLR2/4 overexpression, through the same pathways as exHSP60. In rats subjected to LAD ligation, the released enHSP60 contributed to cytokine production and TLR2/4 overexpression in the ischaemic myocardium.
Extracellular HSP60 induces cytokine production via TLR4-MyD88-p38/NF-κB pathway, and up-regulates TLR2/4 expression via TLR4-MyD88-JNK/NF-κB pathway. Both pathways contribute to myocardial inflammation induced by ischaemia.
Inflammatory cytokines such as tumour necrosis factor α (TNFα) and interleukin (IL) are secreted locally in the heart or systemically in a broad range of cardiac conditions including heart failure, cardiac ischaemia–reperfusion, myocarditis, allograft rejection, and sepsis-induced dysfunction.1,2They are secreted by virtually all resident cell types in the myocardium, including the cardiomyocytes themselves, as well as leucocytes that migrate into the heart.2Through binding their cognate receptors, inflammatory cytokines have important effects on cardiac function and structure.3The biological stimuli that initiate inflammatory cytokine production in myocardium include myocardial injury, haemodynamic stress, autoimmune reactions and rarely, microbial infections.2Activation of inflammatory signalling may proceed through dedicated cell-surface receptors such as toll-like receptors (TLRs), in response to poorly understood endogenous ligands.2,4
TLRs are highly conserved pattern recognition receptors (PRRs) that recognize invading microbial pathogens and trigger innate immunity.5,6At least 10 TLRs have been identified in humans, and six in mice heart.7Upon binding to specific ligands, TLRs signal through two distinct pathways, respectively, dependent on the adaptor proteins myeloid differentiation factor 88 (MyD88) and Toll/IL-1 receptor (TIR)-domain-containing adaptor protein inducing interferon (IFN)-β-mediated transcription factor (Trif). MyD88 and Trif activation leads to activation of nuclear factor-κB (NF-κB) and IFN regulatory factors (IRFs), which result in the production of inflammatory cytokines.4All TLRs except TLR3 signal through MyD88-dependent pathway. TLR4, but not TLR2, partially signals through Trif-dependent pathway.8Accumulative evidence demonstrates that the TLRs play critical roles in modulating inflammation and tissue damage following non-infectious insults such as myocardial ischaemia–reperfusion.9–13A number of endogenous mediators produced by tissue damage likely serve as ligands activating TLRs in the absence of infectious microbial agents.14Importantly, heat-shock proteins (HSPs) have been identified as ligands for TLRs in isolated cells.15,16
HSPs are a class of molecular chaperones that promote protein folding intracellularly. They may be released into extracellular spaces after cell trauma and interact with adjacent cells or distant cells via bloodstream delivery.17We previously found that HSP60, a mitochondrial and cytosolic protein, appeared in the serum, the cardiac plasma membrane fraction, and on the surface of a subset of cardiac myocytes after permanent coronary artery occlusion in rats.18Furthermore, exogenous HSP60 (exHSP60) induced apoptosis in isolated adult rat cardiomyocytes via TLR4.19ExHSP60 has been reported to induce inflammatory responses through mechanisms both dependent and independent of TLR2 and TLR4 in the immune cells.10,16,20,21Endogenous HSP60 (enHSP60) that appears in the serum after myocardial ischaemia and reperfusion has been found to play a role in activating cardiac inflammation.10However, direct evidence and complete identification of the involved inflammatory signal pathway(s) activated in cardiomyocytes by extracellular HSP60 are still lacking. The present study addresses these issues.
An expanded Methods section is provided in the Supplementary material online.
Rat model of myocardial ischaemia
Myocardial ischaemia was induced by ligation of the left-anterior descending coronary artery (LAD) in anaesthetized rats, as described previously.22The rats were anaesthetized with sodium pentobarbital (60 mg/kg, i.p.), and maintained under anaesthesia by bolus injections of sodium pentobarbital (3–6 mg/kg, i.v.) as required. A neuromuscular blocking agent, rocuronium bromide (0.6 mg/kg, i.v.), was used to allow a more stable preparation. The adequacy of anaesthesia was assessed periodically by testing blood pressure and heart-rate responses to toe pinch. After 4 h of LAD ligation, rats were sacrificed with an overdose of sodium pentobarbitone (60 mg/kg, i.v.), and blood and heart tissue were obtained for study. All animal procedures were approved by the Second Military Medical University, China, in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication, 8th Edition, 2011).
Isolation of adult rat cardiomyocytes
Cardiomyocytes were isolated from adult male Sprague Dawley rats and cultured as previously described, with modifications.23,24Briefly, hearts were digested with calcium-free Krebs–Henseleit buffer containing 1 mg/mL collagenase type 2. Dissociated cells were then filtered, collected, brought back to calcium-containing buffer, pre-plated to remove fibroblasts, and cultured in DMEM supplemented with 10% foetal bovine serum. This procedure yielded 70–80% rod-shaped cardiomyocytes. After 1 h of culture, myocytes were treated with low endotoxin HSP60 for indicated time periods.
Models of cultured rat H9c2 cardiomyocytes
The H9c2 rat-ventricular cell line was cultured as described previously.22When cells were 80% confluent, they were serum-deprived for 12 h for synchronization, and then exposed to HSP60 or ischaemia. Ischaemia was induced by exposing cells to 1% O2–94% N2–5% CO2in serum-free low-glucose DMEM for 12 h. The p38 inhibitor SB203580, c-Jun NH2-terminal kinase (JNK) inhibitor SP600125, NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC), or TLR2/4 neutralizing antibody (anti-TLR2/4) were added 15 min before HSP60 or ischaemia treatment. The MyD88 homodimerization inhibitory peptide (Inhi MyD88) or the control peptide (Cntl MyD88) was added 24 h before HSP60 or ischaemia treatment. SiRNAs were used to specifically knock down the expression of TLR2/4, as detailed in the Supplementary material online.
Quantitative reverse transcriptase–polymerase chain reaction
Real-time RT–PCR was performed to determine the mRNA levels of TLR2, TLR4, and inflammatory cytokines, as detailed in the Supplementary material online.
Western blot and co-immunoprecipitation analysis
Western blot was used to determine the protein levels of TLR2, TLR4, MyD88, and Trif, as well as the phosphorylated and total protein levels of p38 and JNK. Co-immunoprecipitation (co-IP) was performed to examine the interaction between HSP60 and TLR2/4, and between TLR4 and MyD88/Trif.
Determination of NF-κB activation
The nuclear translocation of p65, a subunit of the NF-κB heterodimer, was used as readout for NF-κB activation. We examined p65 translocation by immunohistochemistry and by probing western blots of nuclear extracts for p65.
ELISA analysis for TNFα, IL-6, and HSP60
The TNFα, IL-6, and HSP60 levels in cell-culture supernatant and rat serum were measured using commercial ELISA kits (Enzo Life Sciences), following the manufacturer's instructions.
Statistics and data analysis
All the data were expressed as the means ± SD. Data were analysed by a one-way ANOVA followed by the Fisher's least significant difference (LSD) test to determine the differences between groups. SAS 9.0 statistical software (SAS Institute, Inc., USA) was used for data analysis. A P< 0.05 was considered statistically significant.
ExHSP60 induces cytokine production in cardiomyocytes through TLR4-MyD88 pathway
In primary adult-rat cardiomyocytes, as well as H9c2 cells, exHSP60 significantly increased the TNFα and IL-6 mRNA levels as well as the release of these inflammatory cytokines. Time-course studies showed the greatest increase at 6 h (Supplementary material online, Figure S1). Therefore exHSP60 was incubated for 6 h in the following experiments.
A dose–response was observed for exHSP60 in both adult and H9c2 cardiomyocytes. 5 μg/mL exHSP60 increased the mRNA levels and the release of TNFα and IL-6 more than 1 μg/mL exHSP60. The TLR4 agonist, lipopolysaccharide (LPS, 1 μg/mL), was used as a positive control. LPS significantly induced cytokine production in both adult cardiomyocytes and H9c2 cells. To exclude the possibility that endotoxin contaminated in the recombinant exHSP60 may induce inflammation, treatment with heat-inactivated HSP60 (1 μg/mL, heated at 100°C for 5 min) was done. This failed to induce cytokine production, supporting that the observed pro-inflammatory effects are a specific effect of HSP60. (Supplementary material online, Figure S1)
A series of experiments was done to investigate the signalling pathway(s) activated by exHSP60. First, we examined the interaction of exHSP60 with TLR2/4 by co-IP experiments. When the lysates of H9c2 cells were precipitated with antibodies against HSP60, TLR4 but not TLR2 was co-precipitated. In control, untreated cells, only a trace amount of TLR4 was co-precipitated with HSP60. In contrast, in exHSP60-treated cells, a significant amount of TLR4 was co-precipitated (Figure 1Aand B). Secondly, we investigated the adaptor proteins, MyD88 and Trif, which TLR4 signals through. We precipitated cell lysates with anti-TLR4 antibodies, and observed that MyD88, but not Trif was co-precipitated, with a greater amount in exHSP60-treated cells (Figure 1C). Thirdly, we examined the activities of p38, JNK, and NF-κB, the effector molecules downstream of the TLR4-MyD88 pathway. These studies showed that p38 and JNK were activated by exHSP60, as shown by the increased phosphorylation of p38 and JNK (Figure 1Dand E). NF-κB was also activated by exHSP60, as shown by p65 translocation from the cytoplasm to the nucleus (Figure 1Fand G). Fourthly, we examined the effects of inhibiting upstream signalling molecules on exHSP60-induced p38, JNK, and NF-κB activation, as well as cytokine production. The results (Figure 1H–K) showed that TLR4 siRNA (100 nM), anti-TLR4 (5 μg/mL), and Inhi MyD88 (100 μM) prevented the activation of p38, JNK, and NF-κB, and significantly inhibited TNFα and IL-6 mRNA expressions in exHSP60-treated myocytes. SB203580 (20 μM) and PDTC (100 μM) also inhibited TNFα and IL-6 expressions. However, the negative control siRNA (NC siRNA, 100 nM), the control antibody (IgG, 5 μg/mL), Cntl MyD88 (100 μM), and SP600125 (20 μM) did not affect the pro-inflammatory effects of exHSP60. Surprisingly, TLR2 siRNA (100 nM) significantly inhibited exHSP60-induced cytokine production, but anti-TLR2 antibody (5 μg/mL) did not show any effects (Figure 1Jand K).
Furthermore, we investigated whether exHSP60 itself increased TLR2/4 expression in the H9c2 cells. The results showed that the mRNA and protein levels of TLR2 and TLR4 were both increased by exHSP60, and reached a maximum at 6 h, remaining elevated for 24 h (Figure 2A–C). To determine the signal pathway that mediated the increase in TLR2/4 expression by exHSP60, TLR2/4 siRNA (100 nM), Inhi MyD88 (100 μM), SB203580 (20 μM), SP600125 (20 μM), and PDTC (100 μM) were used to interfere with the signal transduction. We observed that TLR2 siRNA specifically down-regulated TLR2 mRNA; however, unexpectedly TLR4 siRNA down-regulated both TLR2 and TLR4 mRNA in exHSP60-treated cells. SP600125 and PDTC abolished the induction of TLR2/4 expression by exHSP60, whereas SB203580, at the same doses that inhibited cytokine production, had no effect on TLR2/4 expression. Thus, induction of TLR2/4 expression by exHSP60 is dependent on TLR4, MyD88, JNK, and NF-κB, but not TLR2 or p38 (Figure 2Dand E).
In sum, the above results showed that exHSP60 stimulated cytokine production via TLR4-MyD88-p38/NF-κB pathway, and enhanced TLR2/4 expression via TLR4-MyD88-JNK/NF-κB pathway in H9c2 myocytes.
EnHSP60 in the extracellular space contributes to cytokine production induced by ischaemia in cultured cardiomyocytes through the TLR4-MyD88 pathway
EnHSP60 is normally located in the mitochondria and cytoplasm. HSP60 was barely detectable by ELISA in the media of normal H9c2 myocytes. However, 3.7 ± 0.7 ng/mL of HSP60 was detected in the media after 12 h of ischaemia (Figure 3A). To examine whether the extracellular enHSP60 contributed to cytokine production induced by ischaemia, anti-HSP60 antibodies (10 μg/mL) were used to neutralize enHSP60. This significantly inhibited cytokine induction by ischaemia (Figure 3Fand G).
The association between enHSP60 and TLR2/4, and between TLR4 and MyD88/Trif was investigated further with co-IP experiments. In accordance with the results for exHSP60, a large amount of TLR4 co-precipitated with enHSP60 from ischaemic myocytes. However, no significant binding was seen between TLR2 and enHSP60 (Figure 3B). Additionally, TLR4 co-precipitated with MyD88, but not Trif. The amount of MyD88 bound with TLR4 was much greater in ischaemic myocytes than control cells (Figure 3C). In accordance with the ability of exHSP60 to activate p38 and JNK, enHSP60 contributed to the activation of p38 and JNK in ischaemic myocytes. The activity of p38 and JNK was increased by 13.2- and 3.7-fold, respectively, under ischaemia, which was significantly reduced to 5.3- and 1.6-fold of control by anti-HSP60 antibodies (10 μg/mL). Anti-TLR4 antibodies (5 μg/mL) and Inhi MyD88 (100 μM) also inhibited p38 and JNK activation (Figure 3Dand E).
Signal inhibitors were also used to investigate the role of key proteins in cytokine production. Anti-HSP60 (10 μg/mL), anti-TLR4 (5 μg/mL), TLR4 siRNA (100 nM), Inhi MyD88 (100 μM), SB203580 (20 μM), and PDTC (100 μM) all significantly inhibited both the increase in TNFα and IL-6 production and release, while SP600125 (20 μM) had no effect (Figure 3Fand G).
Ischaemia increased TLR2 and TLR4 mRNA by 3.3- and 2.0-fold, respectively, and correspondingly increased their protein levels by 4.2- and 3.1-fold. Anti-HSP60 (10 μg/mL), anti-TLR4 (5 μg/mL), TLR4 siRNA (100 nM), Inhi MyD88 (100 μM), SP600125 (20 μM), and PDTC (100 μM) significantly inhibited TLR2 and TLR4 overexpression, but SB203580 (20 μM) had no effect. (Figure 4).
The above results showed that ischaemia-induced cytokine production in H9c2 cells was dependent on enHSP60-TLR4-MyD88-p38/NF-κB. In contrast, enhanced TLR2 and TLR4 expressions were dependent on enHSP60-TLR4-MyD88-JNK/NF-κB. These signalling cascades match those activated by exHSP60.
EnHSP60 in circulation contributed to cytokine production induced by myocardial ischaemia in intact rats
In anaesthetized rats, HSP60 was detectable by ELISA in the serum after 4 h of LAD ligation (8.3 ± 1.5 ng/mL), but undetectable after sham surgery (Figure 5A). Co-IP experiments detected significant interaction between HSP60 and TLR4, but not HSP60 and TLR2, in ischaemic myocardium (Figure 5B). Significant binding between TLR4 and MyD88 was detected in both sham and ischaemic myocardium, with more binding in the latter. However, no binding was observed between TLR4 and Trif in either sham or ischaemic myocardium (Figure 5C).
In ischaemic myocardium, the activity of p38 was increased by 53.3-fold (Figure 5Dand E), and the mRNA level of TNFα and IL-6 was increased by 3.7- and 3.3-fold, respectively (Figure 5F). The serum content of TNFα and IL-6 was increased from 16.9 to 81.8 pg/mL, and from 34.0 to 164.7 pg/mL, respectively (Figure 5G). By using anti-HSP60 antibodies (20 μg/kg, intravenously injected 15 min before LAD ligation) to neutralize serum HSP60 that appeared after LAD ligation, we observed reduced activation of p38 and decreased generation of TNFα and IL-6, confirming that enHSP60 (extracellular) in circulation contributed to p38 activation and cytokine production in the setting of myocardial ischaemia (Figure 5D–G).
Similar to our findings in the H9c2 cells exposed to ischaemia, increased TLR2 and TLR4 expressions were observed in the ischaemic myocardium. As with the H9c2 cells, anti-HSP60 antibodies significantly decreased the increase in TLR2 and TLR4 (Figure 6).
The present study provides direct evidence that extracellular HSP60 activates TLR4 and MyD88, but not TLR2 or Trif, in cardiomyocytes. We demonstrate for the first time that extracellular HSP60, either exogenous or endogenous (released by ischaemic myocardium), induces TLR2 and TLR4 expression. During myocardial ischaemia, HSP60 released from cardiomyocytes stimulates inflammatory cytokine production via TLR4-MyD88-p38/NF-κB pathway, and up-regulates TLR2 and TLR4 expression via TLR4-MyD88-JNK/NF-κB pathway.
Inflammatory cytokines serve as effectors of innate immunity and function as an early warning system to discriminate self and potential pathogens. Accumulated evidence has demonstrated that they are synthesized not only by immune cells but also by cardiomyocytes in response to ischaemia or mechanical stretch.1The importance of inflammatory cytokines to stressed cardiomyocytes is well accepted; however, the mechanisms that trigger cytokine production are not well understood.
Emerging data have identified the TLRs as critical mediators of cytokine production. TLRs are a family of PRRs expressed by immune cells that recognize pathogen-associated molecular patterns (PAMPs). In mammals, TLRs expressed on antigen presenting cells and macrophages serves as key PRRs with central roles in induction of innate immune responses, which are characterized by cytokine production, as well as the subsequent development of adaptive immune responses.5,6TLRs consist of an extracellular leucine-rich repeat region that is involved in microbial recognition, and an intracellular TIR domain that is necessary for signalling. Recognition of PAMPs by TLRs stimulates the recruitment of TIR-domain-containing adaptors, especially MyD88 and Trif.4,6MyD88 is recruited by all TLRs with the exception of TLR3. Recruitment of MyD88 activates the IL-1 receptor-associated kinases (IRAKs), which interact with TNF receptor-associated factor (TRAF) 6 and in turn activate transforming growth factor-β-activated protein kinase 1 (TAK1), a mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK). Ultimately, the MAPKs (ERK, JNK, p38) and the transcription factor NF-κB are activated, leading to the expression of inflammatory cytokine genes.4,6Trif is recruited by TLR3 and TLR4. Recruitment of TRIF leads to the activation of NF-κB, MAPKs, and the IFN-inducing transcription factor IRF3.6However, in the current study, TLR4 activated only the MyD88 pathway, but not the Trif pathway. Both the MyD88- and Trif-dependent pathways lead to a common final outcome, cytokine production, which contribute to innate immune responses.
In addition to immune cells, TLRs have been found in various cell types including cardiomyocytes. Six types of TLRs (TLR2, TLR3, TLR4, TLR5, TLR7, and TLR9) have been reported in murine cardiomyocytes.7Using the method of real-time PCR, we detected the expression of nine types of TLR (TLR1∼9) in cardiomyocytes isolated from adult rats, as well as the H9c2 cell line (data not shown). The present study showed that MyD88 but not Trif was co-precipitated with TLR4 in rat cardiomyocytes, despite the abundant expression of Trif (Figure 2B), suggesting that TLR4 recruits MyD88 but not Trif in response to HSP60 binding. In parallel with the increased recruitment of MyD88 to TLR4, we observed activation of p38, JNK, and NF-κB, as well as enhanced expression of TNFα and IL-6. These results were in accordance with the effects of TLR4-MyD88 signalling in immune cells as described earlier.4,6Additionally, we observed that the increase in TNFα and IL-6 was abolished by the inhibition of either p38 or NF-κB, but not by JNK inhibition. This is in agreement with the pro-inflammatory role of p38 and NF-κB and anti-inflammatory role of JNK described for cardiomyocytes,25,26but in contrast to the pro-inflammatory role of JNK described for immune cells.27,28
Studies with TLR deficient mice have demonstrated crucial roles of TLR2 and TLR4 in ischaemia–reperfusion injury-mediated inflammatory responses in the heart.4However, there are two questions here. First, how do TLR2 and TLR4 become activated by ischaemia–reperfusion in the absence of microbial pathogens? Secondly, whether TLR2 and TLR4 on cardiomyocytes or on infiltrated immune cells, or both, contribute to cardiac inflammation. Previous studies performed mainly in immune cells identified a number of endogenous molecules that induce cytokine production via TLR2 and TLR4, such as HSP60, HSP70, and hyaluronan.15,16,21,29Among these molecules, HSP60 was found by us to be abnormally translocated to cardiomyocyte plasma membrane and the extracellular space after myocardial infarction,18at least in part via exosomes.30This suggests the possibility that HSP60 may activate TLR2 and TLR4 located on the cell surface of cardiomyocytes, as well as immune cells in circulation or infiltrated into the myocardium, and this is supported by the findings of the current study.
In adult-rat cardiomyocytes, extracellular HSP60 had pro-apoptotic effects dependent on TLR4 but not TLR2.19In neonatal-rat cardiomyocytes, recombinant HSP60, at the same concentration used in the current study, was found to activate IRAK-1, a kinase critical for the innate immune signalling including that of TLRs, supporting the inflammatory actions of extracellular HSP60 on cardiomyocytes.10In accordance with the above reports, the present study provides direct evidence that exHSP60 activates the TLR4-MyD88-p38/NF-κB pathway and induces inflammation in cardiomyocytes. We also demonstrated that enHSP60 released from ischaemic cardiomyocytes contributed to cytokine induction via the TLR4-MyD88-p38/NF-κB pathway. Neither TLR2 nor Trif was involved in HSP60 signalling. Furthermore, in intact rats subjected to coronary artery ligation, we detected HSP60 in the serum and observed inhibition of myocardial cytokine expression by intravenously administered anti-HSP60 antibodies, similar to the results obtained in a mice model of ischaemia–reperfusion.10Thus, we conclude that extracellular HSP60 in the circulation contributes to cytokine production in ischaemic myocardium. HSP60 via TLR4 activated production of inflammatory cytokines by the cardiomyocytes themselves. This makes the cardiomyocytes potentially a significant source of inflammatory cytokines in addition to the recruited inflammatory cells expressing TLR4, which may also be activated by HSP60. Increases of serum HSP60 have been reported in a number of clinical conditions, including coronary artery disease and acute myocardial infarction.31,32The binding of HSP60 to TLR4 on cardiomyocytes may represent an important trigger mechanism for cardiac inflammation in these diseases.
The present study to our knowledge is the first to report that HSP60 induced TLR2 and TLR4 expression in cardiomyocytes, through TLR4-MyD88-JNK/NF-κB pathway. The overexpression of TLR2 may partially contribute to HSP60-induced inflammation, as evidenced by the inhibition of TLR2 siRNA on cytokine generation. Previously, the TLR4 ligand LPS was reported to enhance TLR4 expression in neonatal-rat-ventricular myocytes.33TLRs overexpression in response to TLR ligands including HSP60 and LPS may amplify the TLR-mediated signalling and cellular effects.
Extracellular HSP60 has been found to cause cardiac myocyte apoptosis and may be a factor in the progression of heart failure.18,19Here we provide new insights into the underlying mechanisms by which HSP60 causes cardiac myocyte inflammation. The current work shows an unexpected interaction between HSP60 and TLR2/4. HSP60 did not co-IP with TLR2, but knockdown of TLR2 expression with siRNA blocked the increase in inflammatory cytokine expression mediated by HSP60 and TLR4. Similarly, HSP60 increased the expression of both TLR2 and TLR4, which was dependent on MyD88, JNK, and NF-κB. HSP6–TLR4-mediated cytokine production depended on the activation of MyD88, p38, and NF-κB. TLR4 signals through MyD88 and Trif,6but in the current study only MyD88 is involved in signalling. Thus the current study provides several novel findings with regards to the complex interaction of extracellular HSP60 and innate immunity.
Supplementary material
Supplementary material is available at Cardiovascular Researchonline.
Conflict of interest: none declared.
This work was supported by the National Basic Research Program of China(No. 2009CB521902); the National Natural Science Foundation of China(No. 30870906, 31071023); the Pujiang Project of Shanghai, China(No. 08PJ14001); and the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, China(No. [2008] 891).


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El Dorado can't stop Poole, but beats Esperanza
YORBA LINDA – Esperanza running back Troy Poole ran for 230 yards and a touchdown on 29 carries and caught a touchdown pass in the Aztecs' Century League showdown with district rival El Dorado on Friday at Yorba Linda High.
But despite Poole's performance and being down two touchdowns early, El Dorado stepped up big when it mattered most and came away with a 24-21 victory.
"They hung in there and played hard," El Dorado coach Shawn Racobs said. "Obviously our goal is to make the playoffs, and this puts us one step closer."
The Golden Hawks improved to 3-3 overall and 2-0 in league, keeping them tied for first place with Foothill. The Aztecs (3-3, 0-2) find themselves in a desperate situation with four games remaining.
A 20-yard touchdown pass from Troy DeLuca to Poole followed by a 25-yard interception return for a score by Austin Steele put Esperanza ahead 14-0 in the first quarter
A 38-yard field goal from Dillon Lawson early in the second quarter got the Golden Hawks on the board.
El Dorado's Hayden Dunn then hauled in a 17-yard touchdown pass from Cory Wildhagen to make the score 14-10 at halftime.
Wildhagen and Dunn hooked up again in the third quarter, this time for 22 yards to give the Hawks a 17-14 lead.
Poole then scored on a 17-yard run to get the lead right back for the Aztecs.
Then the Aztecs self-destructed.
El Dorado started a drive on its 30-yard line with 11:50 left in the game.
Two personal foul penalties and pass interference penalty on the Aztecs helped set the Hawks up with a first-and-goal from the 5.
Two plays later, Dunn scored from 2 yards out to give the Golden Hawks the lead for good with 10:12 left.
Two sacks and an interception from Kyle Eckart in the Aztecs' final two drives helped put the game away for El Dorado.
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Magento Connect 2.0
Magento Connect 2.0
Connect your webstore to Pinterest service with Magento Pinterest extension. "Pin it" buttons on all product pages allow customers to pin items to their Pinterest boards. "Follow me on Pinterest" button connects users to your Pinterest account and help them keep track of your store updates.
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• Pin it button for all store products
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For now Pinterest is one of the fastest growing social networks in the world. Pinterest mission is to "connect everyone in the world through the things they find interesting". Users can browse various sites, "pin" and "re-pin" images to their own collections.
Magento Pinterest extension ensures full integration between your webstore and Pinterest network. "Pin it" button will appear on every product page. If you create a Pinterest account for your store, "Follow me" button will be displayed for visitors to join you and follow up your Pinterest activity.
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text:
Outdoor Research Hoof It L/S Tech Tee
Retailers' Descriptions
Here's what other sites are saying:
Outdoor Research Men's Hoof It L-S Tech Tee DECENT FEATURES of the Outdoor Research Men's Hoof It Long Sleeve Tech Tee Light weight Quick drying and wicking Dri-release fabric manages moisture Fresh Guard odor neutralization The SPECS Weight: (L): 6.8 oz / 192 g Fit: Standard Fabric: Dri-release fabric: 85% polyester / 15% cotton This product can only be shipped within the United States. Please don't hate us.
- Moosejaw
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The Outdoor Research Hoof It L/S Tech Tee is not available from the stores we monitor. It was last seen July 3, 2013 at Moosejaw.
If you're looking for a new long sleeve performance top, check out the best reviewed current models.


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Sold Out $10.00USD
Details: Soft & stretchable scalloped lace bralette in black, no padding, lined on the inside with non-adjustable straps. One size but stretchable, fits most 32" - 36". 95% Nylon, 5% Spandex. Imported
Care: Hand wash cold
|One Size
|A-C cup
Be the first to get the latest on new arrivals, sales & more
© 2017 NYCT Clothing. California, USA.


text:
47 year old male treated for forehead wrinkles with Botox
169 of 1501
Was this photo helpful? {{ voteCount + ' other' + (voteCountOthers == 1 ? '' : 's') }} found this helpful
Botox used to reduce wrinkles of the forehead in a male patient.
By uploading this photo, Benjamin Barankin, MD, FRCPC has agreed with RealSelf.com policy that they've secured proper patient consent.


text:
Mercedes-Benz 560SEC for Sale
Selected Filters:Type of Ad: Vehicles for Sale Make: Mercedes-Benz Model: 560SEC Remove All
1987 Mercedes-Benz 560SEC
Jupiter, Florida
I This 1987 Mercedes-Benz 560SEC has 93k miles and is powered by a 5.6L V8 paired to a 4-speed automatic transmission. Originally sold for over $69k,... More
1987 Mercedes-Benz 560SEC
Santa Teresa, NM
Car runs extremely good! A/C works and original mats and interior.... More
1988 Mercedes-Benz 560SEC SL
Punta Gorda, FL
... More
1989 Mercedes-Benz 560SEC
Sherman, TX
1989 MERCEDES-BENZ 560SEC ~good driver~ EXTERIOR: The body is very straight and with the exception of a small dent in the driver door I would say... More
1990 Mercedes-Benz 560SEC
Englewood, CO
I purchased the car in 2012 and have carried out numorous repairs and refurbishment to the car. The car has been repainted by an... More
1991 Mercedes-Benz 560SEC 1 Owner ALL Original
Los Angeles, CA
Dear Buyer. This car was a fathers day gift for me from my wife. It was purchased from the showroom floor where it was featured... More
1988 Mercedes-Benz 560SEC Previously owned by Johnny Carson
Chino, CA
1988 Mercedes 560 SEC originally purchased from Beverly Hills Mercedes new in 1988 by none other than the king of late-night himself Johnny Carson... More
1991 Mercedes-Benz 560SEC 560SEC
New Hyde Park, NY
We have a beautiful Mercedes Benz 560SEC for sale. With an impressive 47540 miles this car is ALL ORIGINAL in white with grey interior.... More
1988 Mercedes-Benz 560SEC SL
Orlando, FL
DO NOT miss this end of the year special!!! This car was $14,995 and now it is $11,375. Save $3,620 NOW! The year... More
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1989 Mercedes-Benz 560SEC 6.0 AMG AMG was founded as a racing engine forge in 1967 under the name AMG Motorenbau und Entwicklungsgesellschaft mbH (AMG... More
1988 Mercedes-Benz 560SEC 560SEC
King City, ON
1988 Mercedes-Benz 560 SEC This 1988 Mercedes 560SEC is one of the cleanest examples of a 560 SEC you will find, only 49,000 kilometers ( 30,440... More
1987 Mercedes-Benz 560SEC
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1989 Mercedes-Benz 560SEC Coupe
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text:
The Crossroads of the Aether
James Christopher Hill has not received any gifts yet
Posted on February 1, 2015 at 9:15pm 0 Comments 0 Likes
Posted on January 26, 2015 at 10:23pm 0 Comments 0 Likes
DaVinci's wings Steampunk Style - They do work and are computer tested and animated.
Sir I am the Staff Sergeant ( Recruiting) for the Scribbler's Den Virtual Pub for writers of the punk genres. We have often a need for a clever cartographer and your maps will be of much interest there. I do suggest you join the group as many Authors would enjoy your undoubted talents. Scope for cooperation perhaps?
Yours Aye Steve
Staff Sgt (R)
© 2017 Created by Hephzibah Marsh. Powered by


text:
Have not tried it yet, but was looking at those rooter-pots Lee Valley
has which are (supposedly) pretty fool proof. Would like to do that so I
can get Elaine some viburnums w/out breaking the bank and don't seem to
have much luck rotting them in pots. And you ARE smart Miss Thang, don't
make me come up there!!
Pam Evans
Kemp, TX
zone 8A
----- Original Message -----
From: Jesse Bell
Sent: 6/21/2004 3:47:36 AM
To: firstname.lastname@example.com
Subject: [CHAT] Air Layering
> Has anybody on this list ever done "air-layering" on trees and shrubs?
> My brother has a nursery in Honolulu and he was here this weekend. He
> was showing me how to propogate trees and evergreens using this method.
> So I tried it. Should know something in about 5-6 weeks. Kind of cool. I
> feel so smart now.
> ---------------------------------------------------------------------
> Support hort.net -- join the hort.net fund drive!
> http://www.hort.net/funds/
Support hort.net -- join the hort.net fund drive!


text:
Harrods Sees Far Reaching Benefits of the Weaker Pound
October 18, 2016 6:55 AM Bloomberg Video
October 18 -- By voting to leave the European Union, Britons have delivered a potential windfall to tourists eager to snatch up goods from luxury retailers like Harrods in London. Managing Director Michael Ward discusses what Brexit means for retailers and Harrods with Francine Lacqua on "Bloomberg Surveillance."
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April 17, 2015 7:12 pm
Download audio
June 9, 2014 — This week join NITP's Bob Leins and Tammy Flanagan, co-hosts of "For Your Benefit", as they open the phone lines for a full hour to take your questions.
Topics may include federal benefits, Social Security and insurance, financial planning, the TSP, the Voluntary Contribution Program and income tax planning.
This is your opportunity to have your questions answered!
Some feds miss out on pay raise as salary compression worsens
Some feds miss out on pay raise as salary compression worsens
Photos of the amazing, moving, important and amusing things happening in the federal community.
A daily update of important moments in the history of the U.S. government.


text:
A small gallery plugin using the built in media uploader and gallery shortcodes.
The Mojo Gallery is a very simple WordPress gallery plugin that aims to help people add a small simple gallery to their site/blog. It makes use of mostly built in functions and shortcodes so is fairly lightweight, with additional features as optional extras.
This is very much a DEVELOPMENT release and should only be used for testing or as a starting point for your own project.
Also I'm sorry I'm new to SVN I normally use git so excuse me if there are loads of stupid commits!
Requires: 3.2 or higher
Compatible up to: 3.3.2
Last Updated: 5 years ago
Active Installs: Less than 10
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Linda Hogan has signed to appear on TLC's Brides of Beverly Hills, even though her engagement is off. Here's the official release:
The sophomore season premieres Friday, November 2 at 10/9c
(Los Angeles, CA) – BRIDES OF BEVERLY HILLS returns! TLC premieres season two on Friday, November 2 at 10/9c. Produced by Planetworks Inc., this 15-episode half hour sophomore season will continue to follow the world renowned bridal stylist Renée Strauss and her colorful team of stylists, including celebrity florist Kevin Lee, as they navigate the unique personalities and demands of the Beverly Hills bride. Season one average 1.2 million P2+ viewers and garnered a 1.0 HH rating for the network that is home to the most popular wedding programming on television.
Vowing to deliver even more glitz, glamour and drama, the season kicks off with the premiere episode featuring the famous reality TV star Linda Hogan. Best known as ex-wife of wrestling legend Hulk Hogan, Linda searches for the perfect gown – even after her engagement to Charley Hill, someone her daughter's age was called off. She believes that if she finds the perfect dress, the perfect man will soon follow.
Later, viewers meet bride-to-be Yvonne Pampellonne who was labeled by international media as the "Boob Bandit" back in 2009. Yvonne has been accused of stealing an individuals' identity to get a breast augmentation. After doctors were able to trace her identify through a serial number featured in her old set of implants, Yvonne was forced to plead guilty to burglary, grand theft and identity theft. Now ready to close that chapter in her life, she is determined to find her dream dress and walk down the aisle with Shane, her fiancé who she has known since childhood.
TLC transformed Fridays into "BrideDay" through airing two hours of original wedding-related content each week, securing the network's #1 rank among all ad-supported cable on Friday night in 2012 among W18-34.
About TLC
TLC is a global brand that celebrates extraordinary people and relatable life moments through innovative nonfiction programming. A top 10 cable network in key female demos, TLC has built successful franchises around the Cake Boss and Say Yes to the Dress brands. In 2011, TLC had 28 series averaging 1.0 million P2+ viewers or more including Sister Wives, My Strange Addiction, Extreme Couponing, Toddlers & Tiaras, 19 Kids and Counting, What Not To Wear and Long Island Medium.
TLC is available in more than 99 million homes in the US and more than 227 million households in nearly 150 markets internationally. A destination online, TLC.com offers in-depth fan sites, exclusive video content, and original editorial covering style, home, food, and more. Fans can also interact with TLC via On Demand services, on mobile platforms, including an iPhone App, and through social media such as Facebook or @TLC on Twitter. TLC is part of Discovery Communications (NASDAQ: DISCA,DISCB, DISCK), the world's number one nonfiction media company reaching more than 1.5 billion cumulative subscribers in 209 countries and territories.
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text: Pinch to zoom Personalised Tinder Sack by Harrow & Green £22$37.75€26.36$28.19 45 customer reviews(45) Favourite Favourite View your favourites first line of address (required) town or city (required) post code quantity personalise & pay by Harrow & Green Location: London, GB read seller's profile see seller's complete range more items from this seller Personalised Ampleforth Christmas Sack by Harrow & Green £22$37.75€26.36$28.19 Personalised Bryanston Christmas Sack by Harrow & Green £22$37.75€26.36$28.19 Personalised Burlington Christmas Sack by Harrow & Green £22$37.75€26.36$28.19 Personalised Box Of Five Handkerchiefs by Harrow & Green £20$34.32€23.97$25.62 Personalised Christmas Sack by Harrow & Green £22$37.75€26.36$28.19 Personalised Dog Treats Jar by Harrow & Green £19.99$34.30€23.95$25.61 view all you may also like PRODUCT DETAILS CUSTOMER REVIEWS DELIVERY RETURNS Made in Britain can be personalised ask seller a question save for later This fabulous personalised tinder sack is the ideal way to store all your fireside tinder for the winter ahead. Not only does it look fabulous, it's extremely practical too, being spacious enough to hold the biggest stash of tinder wood. Designed and printed by Harrow & Green in our studio in London, this tinder sack is made of top-quality hessian and features our original 'tinderpress' design which can be personalised with your address and post code. The address appears in black capitals at the top of the design, with the town/city and postcode appearing in smaller capitals directly below it. Simply include the address, town/city and postcode in the spaces provided when you order. The ideal house warming gift, or maybe just buy one for yourself! made from: Hessian dimensions: 80cm x 50cm product code: 152356 45 customer reviews see more customer reviews read about our product rating system ratings guide trusted independent customer feedbackforum excellent bad good poor This product is dispatched to you by Harrow & Green. You have 28 days, from receipt of cancellable goods, to notify the seller if you wish to cancel your order or exchange an item. Please note: goods that are personalised, bespoke or made-to-order to your specific requirements, perishable products and personal items sold with a hygiene seal (cosmetics, underwear) in instances where the seal is broken are non-refundable, unless faulty. Read more about our returns policy.Should you choose to return or exchange your order you will need to deliver the item(s) to the UK, where this seller is based. Harrow & Green's return policy Please see notonthehighstreet.com's returns policy.


text: ×Close Product Image 1 of 1 Hidden Cities The Discovery and Loss of Ancient North American Cities See image Hidden Cities The Discovery and Loss of Ancient North American Cities By Roger G. Kennedy × Added to Cart List Price $23.99 Your Cart ( items) Cart total Continue Shopping CHECKOUT NOW Choose a format: Trade Paperback Format: Trade Paperback Free Press | 384 pages | ISBN 9781451658750 | June 2011 List Price $23.99 This title is temporarily out of stock, please check back soon. Browse Related Books Social Science > Anthropology > General Buy from another retailer Amazon Barnes & Noble Books-a-Million IndieBound Walmart Get a FREE eBook when you join our mailing list. Plus, receive updates on new releases, recommended reads and more from Simon & Schuster. Email Address Free eBook available to NEW subscribers only. Offer redeemable at Simon & Schuster's ebook fulfillment partner.Offer expires in three months, unless otherwise indicated.See full terms and conditions and this month's choices. About the Author Roger G. Kennedy BECOME A FAN BOOKS


text:
Improved Outcomes for
Your Patients and Your Practice
Privia works with top physicians to deliver clinically integrated prevention, wellness, and care management programs to patients. Privia's role in population health management is to support patients in-between office visits, helping them implement their doctors care recommendations. Privia doctors are able to increase their revenue and enter into better arrangements with payers by implementing these programs and delivering high quality care.
Generate additional revenue for your practice
Privia's health management programs enable your practice to generate significant additional income for improving the quality, efficiency, and your patient's care experience. By joining together with other high performance physician practices in the Privia Quality Network or Privia Medical Group, you'll have a unique opportunity to be a part of a group of leading providers who are transforming medicine — and who are being appropriately rewarded for the significant value they create.
Learn more about Privia Medical Group
Learn more about our Clinically Integrated Network and ACO, Privia Quality Network
Join the Leading Physicians In Your Field
Privia is a network of top physicians who are highly respected in their communities and are committed to providing their patients with a modern, proactive healthcare experience that's focused on wellness. As a Privia affiliated top doctor, you'll have the opportunity to participate in and build relationships with other doctors in Privia's exclusive physician network, and you'll benefit from Privia's marketing outreach to patients in your community. As healthcare transforms from volume-based to value-based care, you'll enjoy having a practice development partner who helps you implement population health management programs, streamlines your practice management, improves your revenue cycle performance, and helps you acquire and retain patients by providing a best-in-class healthcare experience.
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text:
Motor transport tests
Found: 1 products← Back to category "Production testing"
Service of branding of transpor
Service of branding of transport The advertizing agency Seventh Heaven LLP in the market of Kazakhstan carries out the activity since 2003. Speci Read more
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Sou brasileira, estudante de letras e tive o prazer de ler esta obra fantastica, que muito me fascinou.Já li alguns classicos da literatura mundial mas nenhum autor conseguiu me prender tanto como Larssson,nunca vi um autor dar vida a tantos personagens numa obra sem perder o climax.A humanidade perdeu um grande escritor.
– nill araujo, 3 June 2011
I still have not found a mass-market or trade paperback of "Girl Who Kicked the Hornet's Nest" in the US. It is available in England and elsewhere but not here.
– Craig-USA, 25 May 2011
I can't either here in Canada! I have both the first 2 in soft and the last in hardcover. Harder to read but still couldn't put it down!!
– Rosie, 28 May 2011
Stieg Larsson is fake!
– FÄALWDAF, 23 May 2011
Why would you say that?
– Boompsii, 1 June 2011
Please, please don't let the Americans make a movie version. Not only do they manage to turn every story into something that centres on American ideology; their movies are mostly tacky and tasteless. Considering what the Americans are attempting to do to Wikileaks and Julian Assange; it would be the ultimate insult to Steig Larson and the height of irony, to have that secrecy obsessed country produce such a movie.
– Cowlad – Perth Australia 20 may 2011, 20 May 2011
sucks for you because they are already making it starring daniel craig
– Wakethefallen- THE U.S.A, 21 May 2011
I won't see the American version, don't need to. It was done right the first time.
– vaughn1111, 1 June 2011
Steig could your next book be about Gorge Soros.
– , 19 May 2011
Steig died tragically in 2004. Look at the site.
– Jeremy, 19 May 2011
You mean rupert murduch, right?
– vaughn1111, 1 June 2011
Recently read that Stieg Larsson was a fan of Harlan Ellison and did an interview with Ellison for TT.
Does anybody have a link to that interview? It would be fascinating to read…
– T – Pasadena, CA, 19 May 2011
Can you tell me when the book "The girl that kicked the hornets nest" will be released in paperback? I have the other 2 in paperback and would like to have this one in paperback if I could. Thank you very much.
– Gail Bays, 19 May 2011
It's been out for a while Gail.
– Jeremy, 19 May 2011
What I believe she is referring to is NOT the large print edition.
– Carli, 20 May 2011
You can buy it from Angus and Robertson
– Boompsii, 1 June 2011
It's not released in the vintage black lizard format yet. Random House says they plan two releases but didn't say when.
– Cindy, 16 June 2011
watched all three movies. I thought that these movies great. really enjoyed them. i was in the library and picked up a novel to read. got home and saw it was the girl with the dragon tattoo. wow. the book saId that the author had passed away. my condolences. i had seen the the movie the gril with the dragon tatto on the shelf but didn't pickup it up. today i went to see about checking it out and it had been placed on hold ten times.
– terrence walston, 10 May 2011
All of the movies are on netflix instant watch if you wanted to get them sooner, But i have to say the books are much better than the movies! (not that the movies are bad but the books are great!)
– W.R., 11 May 2011
Just finnished the third book .came on net to see if there were any follow ups and was upset to find out the writer had passed away a great loss to all including us readers
– Ronald Spencer QLD Australia, 8 May 2011
Great loss to his partner, too.
– Leif Skogfors, 15 May 2011
Yes, it's very sad. A very talented man, and true to his cause.
– Jeremy, 19 May 2011
The trilogy is very compelling to read from the beginning of the first book to the end of the last book, the narrative is neat and polished, the dramatic tension never lets it go, and the character develpment is great in the cases of the main stars Lisbeth Salander and Peter Bllomkvist, in every book however I see some shortcomings like movie action scenes difficult to believe and references to the same issues over and over again. It is advisable to read them in the proper order so as to get a better perspective since in each volume there are references to events described in those volumes preceding them.
– Manuel Gwiazda, Argentina, 30 April 2011
*Mikael Blomkvist
– Boompsii, 1 June 2011


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CHATTANOOGA, TENNESSEE -- I ordered the custom dentures from the company. The day they gave them to me I informed them the dentures did not fit. They told me it was fine and come back in 2 weeks and they would do a remake if needed, and adjust it otherwise. I used my old dentures for the 2 weeks and when I returned they told me they would only fix any sore spots I may have and replied 'I dunno what to tell you' when I said they didn't fit. Then a man came in and said the new teeth were same as the old and I said 'no look there man, it's the gum that's too high'.
The man then turned and bent over about 12 inches from my face and shouted 'DON'T CALL ME MAN! YOU CAN CALL ME SIR WITH RESPECT'. I could smell alcohol on his breath and was very nervous about his aggression because he was a very tall large man. I said 'man is just an expression, I didn't mean it with disrespect, just trying to show you the problem area on the denture'.
The man then continued shouting and said 'Get up and get out of here! GET OUT! HIT THE BRICKS BUDDY!' I was afraid to say anything else because he was red in the face and obviously drinking so I got up and left. As I left I noticed there were at least 2 other customers in the cubicles beside mine who heard the exchange. Also my fiance was there with me. At the front we asked them to put in writing that they were refusing to make the adjustment and the receptionist stated 'I ain't giving you nothing in writing, bye'.
There were about 12 customers in the lobby who heard this exchange as well. I have never been treated this way in my life and I cannot remember being that afraid for my safety. He frightened me so much that after I left when I realized he still had the teeth the company made that did not fit, I was afraid to go back so I just called and asked. I was placed on hold then the line went dead.
I will tell you that after having thought about this overnight I am getting very upset. I paid for custom dentures that would fit, and I was given something that I cannot wear at all. I want custom dentures that fit... I paid for it. I do not want any drama about it, I did not pay for drama and intimidation. The part that unsettles me most about all of this is that I am 26 yrs old but I noticed many of your customers are senior citizens and I should really hope and pray that this office is not treating them the same way they treated (attacked) me if they try to get them to stand behind their quality promises.
I have looked online and found quite a few testimonials from consumers that are almost the same as mine. Very unsettling. I am hereby demanding in writing that Affordable Dentures refund my money because they not only didn't provide dentures that fit, but they kept them when I tried to get them to make them fit. I will cc my attorney on this email in case Affordable Dentures decides to outright scam me.
FT MYERS, FLORIDA -- I went in for a consultation. The reception, nurse and dentist were all very pleasant, but when the other dentist came in, I assumed he was the boss. He was very unhappy with the fact that I didn't want him to pull all of my teeth and give me upper and lower dentures at the cost of $4000.00. He proceeded to yell at me and tell me, "Oh, ok you are the dentist. I see what you want. You want happy teeth." His temper tantrum went on for about 15 minutes. All the while, there were patients in the other rooms and a waiting room full of patients.
This guy is a miserable, excuse for a human being. I would pull my own teeth out with a rusty pair of pliers before I ever allow him to touch me. I will be sure to tell anyone in need of dental care to steer clear of Affordable Dentures on Cleveland Ave. in Fort Myers, Florida!
CARTERSVILLE, GEORGIA -- It took over 2 months to get my partial and it still does not fit. I had to have two extractions and they didn't bother to tell me that after my gums shrunk, I would have to have the partial redone at an additional cost. When I was consulting I specifically asked if I should have the extractions done first and was told that they could be adjusted. I was told it would take two weeks to get them back.
The first set, they had failed to tell the lab about the extractions and so it was another two-three weeks to get the second set done. That was just the metal base which fit the second time but after the teeth were put in, it no longer fits. The staff is rude and could care less if you're satisfied since they already have your money. Be prepared to wait an hour to an hour and a half even if you have an appointment.
If you're considering this place, save your money and go to a real dentist. After you pay all their individual charges, you'd come out cheaper. Partials - $950; X-ray - $80; Extractions - 1 @ $90, 1 @ $140; Realign - $170. Total for one partial - $1630. And that doesn't include the 3 extra trips due to their error.
ZANESVILLE, OHIO -- In January of 2007 I went to affordable dentures in Zanesville, Ohio to have my teeth extracted and get my first set of dentures. I paid for the best package and what I got was a nightmare. I was told they can only extract half your teeth at one time, which is not true. Many places do them all at once, I found out later, but here's what happened.
They extracted my top teeth and sent me home with a prescription of pain medication that I was allergic to, even though I informed them repeatedly about this beforehand. When I tried to contact them in severe pain that Friday as soon as I got home, they didn't get back to me until Monday.
I was scheduled to get my top teeth done on Monday. They called me in the correct pain meds and then rescheduled me for Wednesday due to the 2 inches of snow they got, they were closing. Wednesday they called me and rescheduled me for Friday stating they were playing catch-up due to being closed Monday and Tuesday from the 2 inches of snow. Friday they finally seen me, but due to the medication in my system, 4 of my front bottom teeth wouldn't numb. I insisted he wait and let me stop the meds for a few days and let me return, so they could be numbed.
The dentist told me no, he couldn't stop now! So he pulled 3 teeth that were not numb while I screamed and cried and fought him and then cut the last one out that was also not numb! When I walked out the door, I had blood all over my face, I was shaking in shock, crying and collapsed into my son's arms, who had to physically carry me to the car. The doctor was insane, the women working there were witches and the whole atmosphere was a terrible environment.
I had to go back to that horrible place for my fittings as the swelling went down and these people were awful. They violently force the dentures into your mouth, even an 80 year old man and a woman in her 70's in other rooms moaned and cried while only getting replacement dentures! These people are heartless and cruel! I recently received a notice that they have a new dentist there now. No doubt the old one lost his license, I hope! Beware, the same evil women probably still work there!
I should have sued, but instead I settled for my money back and was given it from their corporate office happily after my story! Beware please, you will wait, wait and wait some more, they will bump you and bump you so they can make room for new patient money, they will treat you very brutally and leave you wondering how they're still in business. Don't go there unless you want a cheap fix and can endure the pain and abuse from these people and end up with dentures that are terrible and plan ongoing back 50 times until you give up. They don't know what they're doing!
I feel bad for the new dentist - he's in for a surprise with that staff!! He'd do well to fire mostly all of them and hire new people!!!
WACO, TEXAS -- Despite the fact that I have very good dental insurance, I have not been able to afford the extractions and upper dentures I desperately needed. Every time I thought I had saved enough, I would receive another estimate from a dentist with designer office furniture and gorgeous wallpaper and carpet that was several thousand dollars beyond my budget as a public school teacher. Finally, I discovered Affordable Dentures and their out-of-the-box philosophy. There are no bells and whistles. They simply do what needs to be done for a reasonable price.
The office is very clean and pretty, but not "designer" quality. The people are very polite and nice. Do you have to wait? Yes. One way the practice saves money is by not scheduling appointments. I made it a point to show up well before the office opened. I was one of the first to sign in. I was seen about an hour later. They immediately took a panoramic x-ray, and I was examined. My options were then discussed. I had done my research, so I knew that I wanted 9 extractions and an upper denture. I chose the non IV conscious sedation.
When the plan had been decided upon, I was given two prescriptions to have filled at my pharmacy. One for pain and one for antibiotics. I was told to return at 1 pm, so I went out to lunch. When I returned, I was given a Valium-type pill and the receptionist and my ride kept an eye on me in the waiting room.
About an hour later I was given an additional amount and thirty minutes later, a little more. I was feeling comfortable, but I didn't really feel drugged at all. Just calm. At 3:20 or so, I was taken back, and I have no real memory of the procedure. The next thing I know, I am being wheeled out of the office by my ride and going home. I had the upper denture in and careful and complete instructions about how to care for myself and my dentures.
The dentist (Dr. **) even called me a couple of days later to check on my progress. It was a good experience overall, and I am very pleased. They did not file my insurance. I paid it all upfront, and I am filing my own paperwork. They provided the paperwork I needed to do that.
The denture I purchased was the economy package. This is the least expensive option. I was given a temporary denture that looks very much like some permanent ones I have seen, and I will receive my permanent one in about six months when my gums have healed. Yes, there will be follow up visits, but I did have it all done in one day. I had my first adjustment and the woman who did the adjustments was very nice and she did a great job. If you do your research and know what to expect, you will be very pleased. Affordable Dentures in Waco, TX provides a safe and affordable alternative if you need dental care and struggle to afford it.
MYRTLE BEACH, SOUTH CAROLINA -- Let me start by saying a extreme lack of money brought me here to start with. I had to have a new set of dentures since the temps. no longer fit. I didn't have $2000 more for new ones. After checking around in Oct.07 I went to Affordable Dentures so excited about getting new dentures (my first set was made in March after extractions). The teeth they made that day looked beautiful but they didn't fit that way. From the start the upper dentures were nothing but problems. There was a gap under the top that let air and saliva in.
I had such a difficult time because saliva and the adhesive continuously poured down over my top teeth. The times I went back I kept trying to explain but they are REAL short on patience if you have any kind of problem. They kept taking more off of them (it just seemed to me they needed something added to fill the gap).
After several months of frustration I went and had them relined. This made it even worse. The upper or lower would not work together. In utter misery, I did not have the money to go buy more somewhere else, I sent a letter to the main office. They gave me a call and said to go back to the Myrtle Beach office. I did but the first thing thing he let me know is this was all he was going to do for me. They first attempted to add something to the original denture, then came back in and told me they WOULD have to be remade. When they came in with the new try-ins the teeth looked horrible they were much longer than the original ones and also are not even (they line up slightly sideways).
By that point, #1 my MAIN concern was that they fit comfortably and #2 they had me so upset I was scared to complain about anything. This I know is my fault, I should have made them do something, but when you are in a situation where you know the people do NOT want you there it affects your feelings. When they brought in the finished product it was so thick and would NOT seat in my mouth but never less they said they were fine and I shouldn't be needing to come back. Well I haven't. I have worked on them myself with a Dremel tool at least trying to make them tolerable to wear.
They are better now than when they sent me away with them but the EXACT same problem is with these. They will not suction and air, saliva and adhesive constantly run over the tops. When I close my mouth or try to swallow I have difficulty.
I finally figured out with my mouth shut my tongue is bound, there is no room for any movement. I have NO idea what to do, I CANNOT afford new dentures, even from the dollar store! I would NOT recommend going to the Myrtle Beach office if you need dentures to wear ALL THE TIME.
You don't realize how drastically it affects the quality of your life. You can't go anywhere without teeth and go very little when the teeth you do have are so unbearably uncomfortable. I only go out when my little boy absolutely needs me to go somewhere. PLEASE SPEND WHAT MONEY YOU HAVE WISELY. The quality and the attitude of this business is in definite need of help. If one person reads this and it helps them to go somewhere else, then God Bless!!!
I just wanted to add a note: The only reason I went to A.D. to start with is lack of money. MY ILLNESS THAT COST ME MY TEETH TO START WITH AND I STILL HAVE HAS TAKEN ALL WE HAD SAVED OR COULD SCRAPE UP. Yes, now I am paying for it in spades!Believe me if I had the money I would have a new set tomorrow.
MIDDLETOWN, OHIO -- If one wants to be treated like cattle this is the place for you! If you are expecting professionalism this is not the place for you. The staff has atrocious attitudes, do not take the time to go over what one should expect, or ask if you have any questions, and if one does question them you can expect an attitude. Being that I am a new denture wearer patient, one would have thought that the time would have been made to make one feel comfortable. Everything was rush, rush, rush. Make sure to read their paperwork very carefully!
NASHVILLE, SOUTH DAKOTA -- Horrible, horrible place. Affordable Dentures provided me with the worst service I have ever experienced with any "professional" medical office. Rude, hostile, uninformed staff, high pressure sales techniques and "Bait and Switch" tactics, provided a dental experience reminiscent of "Little Shop of Horrors." I would not recommend this sham of a dental practice to my worst enemy.
SCOTT, LOUISIANA -- Worst bedside manner and service I've ever seen. This coming from a nurse. Dentures that are pitiful. Can't wear bottoms at all due to size and fit. Tops are full of grinder marks across front of teeth that feel like smeared cement.


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Valley Inn Shakopee
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Free High-Speed Internet
- Continental Breakfast
- Free parking
Photos of Valley Inn Shakopee
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- Free High-Speed Internet
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Miles driven this day: 460
Miles driven in total during road trip: 2,660 (14 days)
States visited: (5) Oregon, Washington, Montana, North Dakota, Minnesota
I'm playing catch up this week. Driving 7-8 hours most days so does not leave much room for updating but I will eventually catch up.
Where is Shakopee You may ask? I too had to look it up on the ...
Day 5 - Tuesday
Forecast was for 18 and overcast today although I think it eventually reached 24. After a very nice brunch at Victor's a Cuban Restaurant we went to the Walker Art Centre. Modern Art - not sure if it was my taste! We watched a presentation call "The Clock" for about an hour. It was made up of ...
Homeward Bound
Sarina: Today we woke up a bit later then we needed (6:45am) and I packed while Care showered. We left our bags with the concierge who called us a taxi while we ate a quick bite. When we were done pretending to eat we were informed that the taxi bus was here....but wait, where were Sarina's bags? It turned out that a different taxi had ended up with Sarina luggage so I had to wait upset while the concierge yelled in Dutch for the other taxi to wait for me at the airport ...
... quite good. We washed it all down with glasses of Goose Island Ale which was a wheat beer and quite florally and refreshing. Towards the end of the trip we went out on deck. Jen had a gin and tonic and was chatting with an American. I had been speaking to a guy from Dakota who was nurseryman, he was telling me about how few holidays he gets and it was his first time on the Mississippi. I also spoke to Chuck our bus driver, who it turns out used to be ...
Empire Builder Evening 1
We were able to get off at the Minneapolis Station and witnessed the beginning of a really good lightning storm. Our friend Dana no doubt was extremely jealous that we were in his town. I was not able to use the line "we know Dana Thompson" to any advantage there, though. Laura turned our little roomette into 2 beds which actually were ...


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Since its flotation in 1989 the shares have risen more than tenfold. They jumped a further 13 per cent to 312p yesterday on the back of a bullish end-of-year statement. Pre-tax profits for the year to September were up 57 per cent to pounds 22.4m, marginally ahead of expectations, but this alone did not explain the jump in the shares.
A key factor in Sage's success is that, once it secures a customer, it makes much more money from after-sales service than it does out of the original contract.
Yesterday's news that sales to new customers were up 20 per cent in the UK business and 24 per cent in the US means that more growth is on the way. Sage already has 161,000 support contracts and last year these operations accounted for pounds 48m of the group's pounds 102m sales.
The other part of the Sage model that is working nicely is its approach to acquisitions. Sage's model is to start overseas expansion with a small acquisition which it then knocks into shape.
Saari, the French group that was acquired last year, is a classic example. It contributed pounds 3m in the year and its margins have already been improved from 7 to 11 per cent with more to come. Similar improvements are hoped for at Sybel, the other French business acquired last month.
There are potential dangers. One is a downturn in new customer contracts, which would have a knock-on effect on support revenue.
Another is that of a larger competitor moving into Sage's niche, although the company's brand name and distribution agreements represent high barriers to entry.
UBS is forecasting profits of pounds 30.4m this year, which puts the shares on a forward rating of 17.
That seems high, but set against exciting prospects it is not too demanding. Good value.
- More about:
- French Politics
- Mergers And Acquisitions
- Software
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Expected: 03/24/2017
Simple contemporary design with an industrial edge.
Small - 82"W x 38"D x 30"H x 188lbs
Large - 94"W x 42"D x 30"H x 210lbs
Product is final sale and non returnable.
For more ideas check out what's the best shape for a dining table?
Color: Brown
Material: Solid Acacia Wood. Iron
Style of Design: Traditional


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Individually Owned Businesses
Need Professional Help? Talk to a Lawyer.
List the name of the owner and the name of the business (Ralph C. Jones, individually and doing business as [d.b.a.] Jones's Texaco). But never just assume that the name of the business is really the same as the name of the owner. For all you know, Jim's Garage may be owned by Pablo Garcia Motors, Inc. (On suing a corporation, see below.) If you get a judgment against Jim's Garage and there is no Jim, it will be worthless unless you take steps to have the judgment changed to reflect the correct name. This can take extra time and trouble. Far better to be sure you sue the right person in the first place.
A few states have liberalized their rules and do not penalize plaintiffs who incorrectly state the business defendant's name. New York allows a plaintiff to sue a defendant under any name used in conducting business if it is impossible to find out the defendant's true name. California allows a plaintiff to correct a defendant's name at the time of the hearing and, in some cases, after judgment, if the defendant is a business person using a fictitious name.
Talk to a Lawyer
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How It Works
- Briefly tell us about your case
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Friends of Center Cemetery has been involved in a wide range of restoration projects ensuring the historic and physical restoration of the Cemetery grounds for some time…Read More
Where art and history meet.
Resting place of many members of Hartford's founding families and with many fine examples of early stone carving.
The Friends of Center Cemetery, Inc. of East Hartford, Connecticut is a non-profit 501(c)(3) organization incorporated November 8, 1989 to promote and preserve this historic town cemetery which was authorized in 1709 as Hartford's second burying ground.
Center Cemetery is one of Connecticut's most interesting and important 18th century burial grounds, and is one of the most striking examples extant of the mixing of Connecticut River Valley sandstones with the eastern granite schists from Bolton and adjacent regions.
James A. Slater
The Colonial Burying Grounds of Eastern Connecticut
The Connecticut Academy of Art


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Published: November 9, 2013 - 05:45 PM
Practice memory work
Q: I'm forgetful when it comes to people. I have a hard time remembering details like names of my co-workers' kids and other personal things. I'm moving into a role where I will be engaging with a lot more people, and am worried that I will seem like I don't care about them. What can I do?
A: Figure out what you want your relationship with co-workers and clients to be like, and then train yourself to make it happen. Whatever style your interactions take, you need to be authentic.
From that perspective, it'll be important to take an honest look at the reasons for your forgetfulness. Create a system to document information about people. Smartphones are a good resource — you can add detailed information into contacts. This is especially helpful for people you don't see often. Then, if you know you'll be seeing someone, you can refresh your memory. But what about people you see regularly and unpredictably? Give yourself a study course to learn some things about people. Practice as you would a vocabulary list if learning a new language. Make it an important part of your day, and give yourself specific goals.
— By Liz Reyer
Minneapolis Star Tribune
Rules on travel time
Q: I recently started providing bookkeeping services to a small local company in technology consulting. I also assist with human resource and payroll-related questions. But I am not always knowledgeable on those topics. The technology company has one employee who works in California providing tech consulting to a client. The employee is hourly. Recently, that employee traveled to New York for meetings with the business owners. The question is whether this employee has to be paid for all the travel time and whether the pay rate should be adjusted for a Sunday meeting. Are these business-specific policy questions, or does employment law come into play?
A: The answer isn't straightforward because the employee is a computer professional.
Generally, the decision to pay or not depends on whether a worker is nonexempt, which generally means hourly, or exempt.
And that dividing line is clear. A truly exempt employee doesn't have to be paid for extra hours on the job as long as the person makes at least $455 a week.
Nonexempt workers have to be paid for all the time they work, and that can include some travel time.
But the rules get more complex for computer workers. Certain exempt computer workers also have to make at least $455 a week. But they also sometimes have that rare distinction of being hourly and exempt. For that distinction to apply, they must earn at least $27.63 an hour — and have specific duties and job titles.
Those jobs include computer-systems analyst, computer programmer, software engineer "and other similarly skilled computer workers," according to federal labor law.
— By Carrie Mason-Draffen
Fixing spellchecker
Q: The spelling correction in my Windows 8 browser, Internet Explorer 10, worked for both of the Windows 8 interfaces (Metro tiles and traditional desktop). But now it's quit working for the desktop. Any suggestions?
A: First, reboot your computer to see if that solves the problem.
If not, try some Internet Explorer 10 adjustments using directions at http://tinyurl.com/qfuljz3 or http://tinyurl.com/ox2xmkx. Or just use another Windows 8 browser such as Google Chrome (http://tinyurl.com/9ccbkzr) or Mozilla Firefox (http://tinyurl.com/cxvxuff). Both have a spellchecker.
— Steve Alexander
Minneapolis Star Tribune


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This app excels in digital photography by allowing your iphone to snap the best photos, combine the rear view photo with the front view photo real time and share with friends via email/Facebook.
By Source Innovative Systems, Inc.
Open iTunes to buy and download apps.
Have you ever wished you could have been included in that special shot, that once in a life time picture? You're never part of the memories/photos that you capture...well not anymore...
With Snap2Pics, you can take a two shots and combine them with our exclusive patent pending Snap2Pics feature. The Snap2Pics feature allows for you to take a one picture then the camera automatically switches to front facing mode and allows you to take a pic of yourself meanwhile combining the two. Awesome huh!
In addition to combining the photos, you can readjust the location of the thumbnail. Snap2Pics also offers the ability to adjust the size of the thumbnail via the settings within the app.
Everyone has a creative mind..Snap2Pics will help bring that creativity out in you, all with a fun, innovative and beautiful memory every single time.
The process to get Snap2Pics approved was very quick. "In fact it took only 4 days from submission to approval in part to the great work on design and development by Copper Mobile, my app development partner", says Rupali Shah owner and inventor of Snap2Pics.
Snap2Pics Features
Taking Pictures
The Snap2Pics feature allows for you to take a picture then the camera automatically switches to front facing mode and allows you to take a picture of yourself meanwhile combining the two.Take pics from either front or rear view camera and take advantage of the snap functionality of the app that combines the front and rear view pics. You can combine the pics in any possible combination.
Adjusting Photos
The "MOVE" feature in the Snap2Pics app gives you the flexibility to move the thumbnail
Pre-set the thumbnail photo size
You will be able to pre set the size of the thumbnail photo.
Share your creations
Whether you want to share show your results to your friends and family or the whole world, Snap2pics has got you covered. Share your photos on Facebook or go the simple route and email them from right within the app.
What are you waiting for ? Try Snap2Pics now and discover that truly you can be part of the memories that you're capturing !
The Idea
I'm sure your family likes to travel and spend time with loved ones cherishing the opportunity the see the world. We love to capture these moments on video and in pictures but the problem is, someone must take the photos and they are always behind the camera capturing the moments and aren't included in any of them. When you're close to nature trekking, on mountains or in the middle of the forest, it is not always possible to start the timer and then run to be part of the photos.
We love sharing our photos with family and friends and everyone has the same comment, "Hmmm...Your Dad is not even part of a single photograph." Our son Aditya did not like that at all. After all it's the memory that we shared together and his Dad is not part of it.
The idea of Snap2Pics came when Aditya said, "Mom, why don't we make an iPhone app that takes the photo of the person who is taking the photo and snap it on the photo that he or she is taking. That gives instant opportunity for that person to share and cherish the moment and be part of the memory".
Additional Information
For additional information about Snap2Pics, including screenshots, a
demo video, and more, please visit http://www.snap2pics.com
YouTube Link
What's New in Version 2.1
Fixed minor bugs.
iPhone Screenshots
Customers Also Bought
- $0.99
- Category: Photo & Video
- Updated: Mar 29, 2012
- Version: 2.1
- Size: 9.3 MB
- Language: English
- Seller: Source Innovative Systems, Inc.
- © Source Innovative Systems, Inc.
Compatibility: Requires iOS 4.0 or later. Compatible with iPhone, iPad, and iPod touch.


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From Wikipedia, the free encyclopedia
|This article does not cite any sources. (June 2009) (Learn how and when to remove this template message)
The stratopause (formerly Mesopeak) is the level of the atmosphere which is the boundary between two layers: the stratosphere and the mesosphere. In the stratosphere the temperature increases with altitude, and the stratopause is the region where a maximum in the temperature occurs. This atmospheric feature is not only associated with Earth: it occurs on any other planet or moon that has an atmosphere as well.
On Earth, the stratopause is 50 to 55 kilometres (31–34 mi) high above the Earth's surface. The atmospheric pressure is around 1/1000 of the pressure at sea level. The temperature in the stratopause is -15 degrees Celsius (5 degrees Fahrenheit).


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Have you hired BGE? Write a review and share your experience!
Description: Drive Pros is a premium pavement maintenance company serving all customers from Halifax to Sydney NS. We only use high quality products and offer expe...more
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Kings Down Golden State 114-106 - Match Report
The Kings got a 28-point performance from Marcus Thornton as Sacramento ran past Golden State 114-106 on Saturday at Power Balance Pavilion.
Sacramento was a favorite by 1.5 points and came through with an ATS victory for bettors. The game went OVER the consensus closing total of 199.5.
Sacramento got 26 points from Tyreke Evans and another 21 points from DeMarcus Cousins. Tyreke Evans contributed 9 assists, while DeMarcus Cousins cleaned the glass with 20 rebounds.
The Warriors got a 24-point performance from Dorell Wright, who was supported by 20 points from Nate Robinson. Stephen Curry had 9 assists in a losing cause for Golden State, while Andris Biedrins grabbed 5 rebounds.
Sacramento trailed at halftime, 54-53.
The rebound battle favored Sacramento as they grabbed 55 boards, while Golden State only had 36.
Sacramento heads into a battle against the Hornets. Golden State, meanwhile, will have their next action against Oklahoma City.


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Last year, the Icelandic version of Asgeir Trausti's In the Silence became the country's most successful debut album by a homegrown artist, outselling even Björk and Sigur Rós, whose global profiles the folk-singer will surely emulate. With the original lyrics – mostly written by his father – translated into English by John Grant, it's clear how closely they suit Asgeir's angelic vocal tones. His acoustic fingerpicking recalls José González, while Gudmundur Kristinn Jónsson's production envelops Asgeir's fragile gifts in delicately wrought arrangements.
Download: Higher; In Harmony; Summer Guest; In the Silence
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While female sexuality appears to be more fluid, research suggests that male gayness is an inborn, unalterable, strongly genetically influenced trait. But considering that the trait discourages the type of sex that leads to procreation — that is, sex with women — and would therefore seem to thwart its own chances of being genetically passed on to the next generation, why are there gay men at all?
Scooped by
jean lievens
onto real utopias