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The new Fashion Outlets of Chicago is an unparalleled shopping environment, where fashion meets art and fine dining! Shop over 130 designer outlets, including Tory Burch, Coach, Prada, Bloomingdales The Outlet Store and Last Call by Neiman Marcus. Located just minutes from O'Hare International Airport.
Chicago Premium Outlets, Aurora
Find impressive savings at 140 designer and name-brand outlet stores, such as Ann Taylor, Armani, Banana Republic, Calvin Klein, Coach, Cole Haan, Gap Outlet, J.Crew, Lacoste, Nike, Polo Ralph Lauren, Salvatore Ferragamo, Michael Kors, SAKS Fifth Avenue Off 5th, Theory, Vera Bradley and more. A recent expansion will bring additional stores in the spring of 2016 making this even more of a Midwest Shopping destination! In Aurora, 37 miles west of Chicago.
Located on the banks of the Fox River, historic St. Charles is known for its great antique shops housed in turn-of-the-century buildings. Visit Century Corners Historic Shopping District and Old St. Charles, where you'll find landmark buildings, unique boutiques and nearly 30 restaurants.
CITY:St. Charles
Goebbert's Pumpkin Farm and Garden Center
Fall: festival with haunted house, corn maze, farm animals, and more. Spring/Summer: large selection of annuals, perennials, vegetable plants, trees, shrubs, aquatic plants, roses, planters, and gourmet food items. Admission is free - cost for some activities.
CITY:South Barrington
Held June 10, July 8, Aug. 12, Sept. 9 and Oct. 14.
The oldest and largest continuously operating winery in Illinois producing more than 50 varietals and 25,000 cases of award-winning wines each year. Come in for a tour and tasting to experience the finest vintages, gourmet cuisine, and luxurious lodging. Tasting room and gift shop open daily. Complimentary tours on weekends. Gift shop featuring wine-related items, specialty gift baskets, glassware, and elegant home decor. Visit our website for events throughout the year including our renowned Oktoberfest.
One of Illinois' top tourist destination, anchored by Nordstrom, Macy's, Lord & Taylor, Sears, and JC Penney. Woodfield has nearly 300 stores, restaurants, and specialty shops all under one roof.
Nestled in the heart of the Fox Valley, and home to dozens of delightful shops, restaurants and galleries is Geneva – a picture postcard. Come stroll along the lush, tree-lined streets and visit a historic town with exquisite dining, boutique shopping, world-class lodging and award-winning festivals. Located 50 miles from downtown Chicago, Geneva is a quick getaway from the hustle and bustle by I-88, I-90 or Metra train.
DuPage County, Chicago's Western Suburbs - 100's of shops, quality merchandise at bargain prices. Craft/collectibles mall.
CITY:Villa Park
Huntley Outlet Center offers over 40 brand name retailers at 25% to 65% off every day, including Mikasa, Reebok/Rockport Outlet Store, BOSE Factory Outlet, The Gap, Carter's and much more.
Enclosed, two-level shopping center featuring Apple, Coach, A/X Armani, Lego, Gilly Hicks and over 150 fine shops & restaurants.
CITY:Orland Park
Open area with both indoor buildings and outdoor displays. Special events are held here nearly every weekend, including the famous Kane County Flea Market.
CITY:St. Charles
Over 150 specialty stores with outdoor ambiance! Nordstrom, Neiman Marcus, Macy's, Lord & Taylor and Sears, The Cheesecake Factory, Maggiano's, The Clubhouse and Mon Ami Gabi restaurants. Voted the #1 shopping center.
CITY:Oak Brook
Visit the Ferrara Pan Candy Company outlet store and see how many ways you can satisfy your candy cravings!
CITY:Forest Park
Chicago Botanic Garden Farmer's Market
First and third Sundays of the month from May until October (9 a.m. to 3 p.m.) Gather seasonal produce, flowers, herbs, and other local, sustainable products at this bimonthly Garden event that is held rain or shine. Talk with vendors and sample fresh fare. Visitors may purchase a special market bag from the Garden Shop to carry their purchases home. Free admission; $25 per car (includes tax) .
A lifestyle and entertainment center offering streetside shopping and dining in a suburban setting. A vibrant shopping alternative including Carson Pirie Scott, AMC-Loews Theater, GameWorks, Laugh Out Loud Improve Theater, and some of Chicago's top restaurants. Store, restaurant, and theater hours vary.
Main Street Promenade Naperville
Whether you are visiting for work or pleasure, Naperville has something to fit your taste. Come and experience big city styles with small town smiles! Discover the charming shops and distinctive restaurants along our tree-lined streets. Stroll the Riverwalk and enjoy the many pieces of public art, fountains and covered bridges.
Located at I-355 and I-55 at the Boughton Rd. exit. IKEA offers home delivery, rental vans, assembly services and an IKEA credit card so that you won't have any worries while you are shopping for your home. Don't forget to visit the restaurant while you are there!
Galena Cellars Winery & Tasting Room
You're in good company. Experience the charm of Galena in suburban Geneva. The newest addition to the Galena Cellars Family, the Geneva Wine Tasting Room & Gift Shop hosts a beautiful oak bar in a light, airy setting. In the warmer months, enjoy live music with wine and cheese on the outdoor patio. Sit. Swirl. Sip & Savor. Relax on our beautiful Wine Patio after shopping Geneva's historic district.
Your sweet tooth is sure to be on alert at Long Grove Confectionary Co. Watch homemade fudge getting made as thick layers of sweet goodness fold into large vats. Inhale the sugary goodness of the famed cocoa concoctions as soon as you walk inside. At Long Grove, the old fashioned confectionary is alive and well, and smelling oh so good.
CITY:Buffalo Grove
Shop Von Maur, Carson Pirie Scott, JCPenney, Target, and nearly 150 specialty shops. Kids love the Yorktown Express train and the free Treehouse Adventure Play Area. Experience exciting tastes, styles and entertainment at The Shops On Butterfield.
Long Grove Confectionery Nut and Candy Factory Store
The former Nut & Candy House is now the Long Grove Confectionery's Factory Store. Along with the store's original kinds of fresh roasted and raw nuts, chocolates, chocolate covered nuts, yogurt covered items, snack mixes and assorted candies, the store will also feature Long Grove Confectionery's famous hand-made chocolates. Bulk, bagged or gift packed items also available.
Didier Farms offers a variety of vegetables and flowers in our greenhouse this spring, and our farmers market in summer. U-pick opportunities are plentiful throughout the summer, weather permitting. During our Pumpkinfest, the last week of September and all of October, we offer hayrides, pony rides, corn maze and an exotic zoo, in beautiful Lake County. Also enjoy the Buffalo Creek Mining Co, pony & camel rides, Animal Land, Pumpkin Playland, kiddie rides, Parakeet Encounter, Pig Races (Sat, Sun & Mon) Pumpkin Patch Café, homemade apple cider & pumpkin donuts made daily. Antique tractor display, fall produce, birthday parties and school groups by reservation only.
Mainstreet shopping & dining destination anchored by Ikea, Macy's and Bass Pro Shops. Ted's Montana Grill, Gordon Biersch and Johnny Rockets are a sampling of the restaurants. Barnes & Noble, H & M, Coldwater Creek and Coach represent the many retailers.
DuPage County, Chicago's Western Suburbs - Choose from a large variety of restaurants from fine dining to casual fare. Catch a movie or browse our wonderful shops.
Welcome to Lynfred Winery Wheeling, a satellite location for Illinois' oldest and largest, continually-operating winery. Since 1979, Lynfred Winery has been offering guests with great wine, food and lodging. We offer wine tastings, flights, and bottles every day of the week. We have received numerous awards for our wine, and we have countless events that go on throughout the year at the winery. We also have a variety of gift and gourmet food items.
One of the nation's leading fashion specialty retailers, offering an extensive selection of contemporary and classic shoes, clothing and accessories for the entire family.
Cooper's Hawk Winery & Restaurant - Burr Ridge
After four years of planning and construction, Cooper's Hawk Winery & Restaurant opened in 2005 in Orland Park, a south suburb of Chicago. The concept was unique. We would create a signature menu with a selection of dishes designed to pair perfectly with our own Cooper's Hawk wines, made in our own private winery. With sophisticated food and exceptional wines, our concept was born. The Cooper's Hawk concept includes four distinct components: an upscale casual dining restaurant, full-service bar, private barrel-aging room, and Napa-style tasting room and retail gift store…all under one roof. Each Cooper's Hawk location is distinctive in layout and décor, but carries the same warmth and elegance that guests have come to expect.
CITY:Burr Ridge
A hybrid between a discount and a department store. Apparel for men, women, children, gifts and accessories.
CITY:St. Charles
Dick's Sporting Goods - Schaumburg
Dick's Sporting Goods leads more athletes to victory by providing the edge they need to perform at their peak. They offer a deep assortment of sporting goods and apparel for baseball, football, hunting, golf, fishing and more.
Great shopping at three department stores: Carson Pirie Scott, Kohl's and Sears, plus a full line-up of more than 140 fabulous shops,including H&M, Pandora & More! Food court and AMC Theatre Chicago Ridge 6.
CITY:Chicago Ridge
Discount Retail Shopping/Grocery.
CITY:St. Charles
Lincolnshire's place for dining, shopping, entertainment and work. Enjoy unique boutiques, the 20-theatre Regal Cinema and IMAX, glow-in-the-dark miniature golf, ceramic painting, outdoor summer concerts and a variety of restaurants.
Over 40 specialty shops, a grocery store, furniture store, and restaurants at this center.
CITY:Crystal Lake
Farm dinners at Heritage Prairie Farm, in Elburn have been a tradition since 2007. All farm dinners include a welcome cocktail upon arrival to the farm with butler-passed appetizers, and live acoustic music. For dinner, an upscale five-course meal, introduced by the chef himself, is served family-style in a charming atmosphere, regardless of the season. Get an inside look at how the farm operates from one of their knowledgeable staff with a private tour of the farm.
The Arboretum of South Barrington
The Arboretum is South Barrington's newest and most exciting place to shop, dine and play. The open-air center is named for the lush treescapes and gardens of its landscape, and for the large tree farm that formerly stood on the site. An upscale mix of shops, restaurants and entertainment venues including iPic Theaters, the ultimate cinema experience and a new way to go to the movies; L.L.Bean's first Midwest outdoor gear and apparel store and the incomparable Pinstripes Bowling/Bocce/Bistro.
CITY:South Barrington
Whether you are new to tea or far along on your journey, you will enjoy discovering farm-fresh teas at Adagio Teas. Come to experience a fresh and flavorful selection from around the world. Tea, tea-to-go, teaware, and tea classes.
Siegel's Cottonwood farm is a fourth generation working farm established in 1909. In the spring and early summer, the farm is open with a garden center featuring flowers, shrubs, and plants. From June 1st thru October, the farm is available for company picnics, group outings, corporate events and fundraisers. In the summer when the crop is ready its extra sweet corn. In the fall its Pumpkinfest time on the farm with playlands, cornfield mazes, new fun attractions, climbing wall, gem mining and much more. And then there is the award winning haunted prison, "Statesville."
CITY:Crest Hill
RAM Restaurant & Brewery - Rosemont
The RAM offers a casual dining atmosphere, including a brew house with adjoining bar/lounge and restaurant areas. Lively environment accompanied by great house-brewed beers and daily food specials.
Hawthorn Mall is home to Macy's, JCPenney, Carson Pirie Scott, Sears and over 140 diverse specialty retailers including: Bare Escentuals, Banana Republic, Barnes & Noble, Disney Store, Gap, LOFT, H&M, Mario Tricoci Day Spa & Salon, Teavana, and Victoria's Secret. Ruby Tuesday and a food court offer over 20 eatery options.
CITY:Vernon Hills
Goebbert's Pumpkin Patch - Hampshire
Ride the wagon to pick your own pumpkin, shop the Harvest Market and visit the Farmers Wife Cafe and Bakery. Goebbert's has a second location in South Barrington.
Cooper's Hawk Winery & Restaurant - South Barrington
After four years of planning and construction, Cooper's Hawk Winery & Restaurant opened in 2005 in Orland Park. The concept was unique. We would create a signature menu with a selection of dishes designed to pair perfectly with our own Cooper's Hawk wines, made in our own private winery. With sophisticated food and exceptional wines, our concept was born. The Cooper's Hawk concept includes four distinct components: an upscale casual dining restaurant, full-service bar, private barrel-aging room, and Napa-style tasting room and retail gift store…all under one roof. Each Cooper's Hawk location is distinctive in layout and décor, but carries the same warmth and elegance that guests have come to expect.
CITY:South Barrington
One of the last actual places of its kind to find copies of thousands of magazines and posters for collectors and enthusiasts alike. Life, Rolling Stone, Motor Trend, Mad, Star Trek, Beatles, if you can remember it....Bob probably has it. All items are very reasonably priced and not only make great gifts but give you your chance to own history. The collection includes periodicals from centuries past. The store also features flags from over 200 nations.
Rocktown Adventures is a specialty outdoor retailer and adventure club. They offer sales, rentals, lessons, guided trips and related apparel for kayaking, canoeing, stand-up paddleboarding, Nordic skiing and snowshoeing. They also carry camping equipment, lifestyle apparel and transportation equipment. Rocktown's mission is to help you gear up and head out. They carry top equipment sold by knowledgeable staff. Their experienced instructors love to share their passion for the outdoors. The trip guides know the waterways and trails like the back of their hand. The shop is conveniently located near an abundance of recreational assets waiting to be discovered by you!
This perfectly-planned town offers beautiful shop and restaurant-lined streets—just like a European town. A 10-screen movie theater, shops including Von Maur, Hot Mama and Dick's Sporting Goods as well as restaurants unique to the North Shore including RA Sushi, Jameson's Charhouse and Ted's Montana Grill. The Glen Town Center is also home to the Kohl Children's Museum, a leading institution for hands-on, interactive learning.
Jeanine A Guncheon Gallery Etcetera
An unusual shop that runs the gamut from art, gifts & vintage. Uniquely individual. Owner and artist, Jeanine Guncheon, has a wide collection of her hand painted bowls, furniture, and one-of-a-kind sculptures. Commission pieces on request.
CITY:Forest Park
Get creative at this crafter's paradise! Beads from around the world, unique rubber stamps & papers, Ranger inks, paints, and stamp pads. Sit at our "crystal bar" and work on your projects. Our friendly staff will be happy to assist you. Classes for all skill levels. Parties are our specialty! Birthdays, bridesmaids, "girls night out" -- we do them all. Come take a look!
CITY:Forest Park
Sephora presents beauty, fragrance & skincare enthusiasts an unparalleled selection of prestige cosmetics that you can touch and try. We offer complimentary color, skincare and fragrance consultations with our beauty experts and free samples!
CITY:Oak Brook
Arlington Heights Farmers Market
Enjoy fresh produce, flowers, cheeses, bakery good, jams, jellies, and more from local farmers. Open June through October.
CITY:Arlington Heights
Maple Avenue Gallery features sophisticated, contemporary works of art that range in price from about $500 to more than $6,000. In addition to the gallery, the company also offers custom framing and art consultations. Every month, Maple Avenue Gallery hosts receptions with live music and refreshments.
Over 130 fabulous stores featuring the latest fashions and trends, including Carson's, JC Penney, Sears and Forever 21. A new Classic Cinemas opened in 2008 showing great movies. Conveniently located on Cermak Rd., west of Harlem Ave.
One of the Midwest's most celebrated shopping malls. With over 140 specialty stores including retail giants Bloomingdale's, Lord & Taylor, Macy's, and Nordstrom, this spectacular outdoor mall features beautiful gardens, landscaped walkways, sparkling fountains and children's play areas. McCormick and Schmick's, California Pizza Kitchen, Cheesecake Factory, and Wilde and Greene offer a superb dining option. Catch a movie at the 13 screen AMC Garden Cinema.
Northbrook Court is home to a powerful anchor alignment: Neiman Marcus, Macy's, Lord & Taylor and a free-standing, 36,000 square foot Crate & Barrel Home Store. Also featuring BCBGMAXAZRIA, Cole Haan, CUSP, Tommy Bahama's, Louis Vuitton, Ralph Lauren, Burberry and Elizabeth Arden Red Door Spa. Dining and entertainment options include P.F. Chang's, Stir Crazy, Di Pescara, and AMC Theatres providing 14 screens.
Above and Beyond Framing and Gallery
Custom framing, art and gift gallery. Featuring Steve Hanks, Tom Lynch and Scanlan.
Discover hundreds of well-known speciality and chain stores, and dozens of great dining locations, along the Route 59 Corridor in Naperville.
DuPage County, Chicago's Western Suburbs - Enjoy unique shopping and dining in Historic Downtown Lombard and the beautiful Lilacia Park. Special events for all ages throughout the year. Free parking.
Stratford Square Mall - Bloomingdale
Stratford Square Mall is a two-level enclosed super-regional shopping center located in the western suburb of Bloomingdale. The center hosts 6 anchor department stores and more than 160 specialty shops.
Located next to Toys R Us, this pickers paradise is 10,000 square feet of antiques, collectibles, vintage, retro and fun stuff! 75 dealers. Open 7 days week! Mon-Sat 10-6, Sun 11-5.
Geneva Commons has a wonderful mix of 75-plus merchants, from familiar favorites to new and unique shops. Spend time shopping, eating and relaxing in this open-air center. Dine at a wide variety of eateries, most of which provide outdoor seating.
St. Charles' newest source for rubber stamps, supplies and classes. Also offering custom invitations and announcements created for your special occasions.
CITY:St. Charles
Art gallery featuring original artwork as well as Thomas Kinkade, H. Hargrove and Giuseppe Armani figurines.
CITY:St. Charles
Tucker Gallery specializes in contemporary American and European artists. Styles and subjects range from traditional to abstract.
Chicago Botanic Gardens Farmers' Market
The Garden welcomes local, sustainable farmers and producers to this public farmers' market on the first and third Sundays of the month from June through October. Admission to the market is free to Garden visitors and will be held rain or shine. Visitors may purchase a special market bag from the Garden Shop to carry their purchases home.
Several restaurants and seven national chains, including Best Buy, K-Mart, Office Depot, Bed Bath & Beyond, and Sports Authority, provide various home and business products.
CITY:Crystal Lake
Incredible place to browse and shop. Glass jewelry, art and more. From Tiffany to Nedobeck.
Home furnishings that combine good design, function and quality at affordable prices.
Different from your average shopping mall, Deer Park Town Center is an open-air lifestyle center providing you with leisurely amenities in addition to some of the best shopping and dining in Illinois. Deer Park Town Center features over 70 of the most exclusive shops and restaurants in Chicago's northwest suburbs. Stores include Apple, Crate & Barrel, Barnes & Noble, Sephora, Gap, Pottery Barn, Restoration Hardware and more. Entertainment includes Century Theatres. Park in front of your favorite store and spend an hour or a day shopping and dining, or catch a movie at the Century Theater.
CITY:Deer Park
A nice selection of fabric, notions, books and threads. Experienced quilters to assist with all your quilting needs. Quilting classes available.
Deer Park Town Center features over 70 of the most exclusive stores and restaurants in Chicago's northwest suburbs. Located at the intersection of Rand (Route 12) and Long Grove Roads in Deer Park, IL, Deer Park Town Center is an easy drive from surrounding suburbs, downtown Chicago or O'Hare Airport. Park in front of your favorite store and spend an hour or a day. At Deer Park Town Center, there's something for everyone.
CITY:Deer Park
An Evanston tradition for 28 years, designer-goldsmith Peggie Robinson and her staff can assist you in your selection of a piece of unique jewelry from their own designs, or help you design a piece just for you.
Barrington Ice House Village Mall Shops and Restaurants
Explore this historic mall, built in 1904, featuring more than 20 specialty shops and Chessie's, an authentic 1915 train station restaurant with its own railroad car.
We sell only products made in the United States! We offer an eclectic mix of common household items from toys to tools, glassware to garden supplies, fashions to food and so much more. When you shop at Norton's U.S.A, you help keep America working!
Forest Park Chamber of Commerce & Development
The Forest Park Chamber of Commerce & Development is dedicated to the continued growth and revitalization of Forest Park's business districts as well as the enhancement of quality of life for both businesses and residents. Investment in the Chamber partners you with a comprehensive group of business owners and residents throughout the community who want to be involved, stay informed and work together towards making Forest Park an even better place to live, work, shop, dine and play. We invite you to join us!
CITY:Forest Park
Known throughout the Fox Valley for top-quality meats, produce, and fresh bakery products. Where service has been a family tradition for more than 70 years in St. Charles.
CITY:St. Charles
Gallery 200 is fast becoming a haven for art lovers as a quality destination for buying and browsing great local art. The gallery, a cultural initiative supported by the City of West Chicago, hosts artist opening receptions, art talks and workshops.
CITY:West Chicago
Afghans, books, baby quilts, birdhouses and feeders, ceramics, goose clothes and geese, southwest artifacts, lawn and garden decor and more.
Stained glass supplies, custom and restoration.
Fox Valley Mall - Centennial Collection
Fox Valley Mall is the premier shopping center in Illinois, featuring 130+ specialty stores and dining options! Anchored by Macy's, Carson Pirie Scott, J C Penney and Sears. Fox Valley Mall has three levels of shopping, a full food court, and a merry-go-round. This mall is a must-shop-stop for everyone! The NEW Fox Valley Train is located on Level 2 near JCPenney.
"A Million Reasons -- A Single Store!" From home decor, clothing, fashions and groceries to home improvement and more.
CITY:St. Charles
DuPage County, Chicago's Western Suburbs -- This sportsman's paradise is a breath of fresh air for outdoors enthusiasts. At Bass Pro Shops Outdoor World in Bolingbrook, you'll find everything you need for fishing, hunting, marine, camping, golf, footwear and gifts. Your adventure starts here!
The 185,000-square-foot retail showroom is an educational and entertainment attraction, featuring a décor of museum-quality animal displays, huge aquariums and trophy animals interacting in realistic re-creations of their natural habitats.
CITY:Hoffman Estates
A specialty food store featuring artisanal, small batch extra virgin olive oils on tap, a variety of balsamic vinegars from Modena, Italy, fresh spices from our spice locker, and a full menu of gourmet food items and accessories. Stop in for a taste and see why customers say wow!
CITY:Long Grove
Warm, friendly service is the hallmark of historic Downtown Wheaton. Stroll the tree-lined streets of this upscale retail and service district and delight in the quaint shops, award-winning restaurants, indulgent day spas and unique boutiques.
World's largest manufacturer of art pottery. Factory outlet salesroom and Haeger Museum open to the public at original manufacturing site.
CITY:East Dundee
Country Decor from Ceiling to Floor! Country chandeliers, country table and floor lamps, sconces, country ceiling lights, punched tin switch plates, and country style curtains!
We are a printmaking workshop open to the public, displaying local artists' original prints at reasonable prices. We also offer jewelry, cards, pottery and one-of-a-kind works.
CITY:Oak Park
Has been making its own delightful signature toffee in Lockport for 30 years. Hollingworth Signature toffees and candies are locally handcrafted with the finest and freshest, natural ingredients. Pure ingredients. Pure indulgence. Pure Bliss.
The City of Lockport has a unique downtown. With a history that drove the economy of Illinois and the Midwest through the early 19th century, Lockport has many historical markers, some visible, some hidden, that attest to its influence.
Lands' End is a direct merchant of traditionally styled clothing for the family, soft luggage, and products for the home. We work directly with mills and manufacturers, eliminating the markups of middlemen.
The Avenue shops are located at Oak Park and Lake Street and across from picturesque Scoville Park. It's All Here! In the "Heart of Oak Park" you'll find specialty shops that offer personal service as the norm -- not the exception! International and local designers of fashion and jewelry, antique shops, galleries and fine restaurants.
CITY:Oak Park
Downtown Crystal Lake/Main Street
Visit our award-winning Premier Main Street which is full of charming shops, delightful restaurants, quality entertainments and life-enhancing opportunities and events.
CITY:Crystal Lake
It happens the moment you turn onto the long, gravel road; it might be the charm of the converted century-old farmhouse filled with handcrafted pottery, homemade candles and other unique finds. It might be savoring the last bite of Blackberry Peach Cobbler after a delicious, freshly prepared lunch in the tearoom. Or it might be the peaceful serenity found only in the countryside. Whatever "it" is, friends young and old, male and female, have been coming back to Emerson Creek to experience it again and again.
Located in the Oak Park Arts District, we feature contemporary folk art, found object art and fine crafts created primarily with vintage, re-used, reworked, recycled and reclaimed materials. You will find truly unique, eco-friendly, "green" art and artful objects for yourself or for gift giving created by Lisa Nordstrom and other intriguing artists. Visit our website for current exhibits and special events.
CITY:Oak Park
Specializing in Counted Cross Stitch and Custom Framing. We have the largest showcase of fabrics, fibers, patterns, kits and accessories in the Midwest.
CITY:Crystal Lake
Unusual treasures and gifts, custom glass flower arrangements and framed art.
Shop more than 140 retail and specialty stores.


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1. Glo Worm
With it's soft body design and light-up head, Glo Worm was the perfect preschool kid toy. I mean who else was going to protect you from the monster that lived under your bed? Definitely not the Fisher-Price Little People!
2. Fisher-Price Gas Pump
Fisher-Price made the perfect toys to introduce us to the real world, and yes, that apparently also included how to pump your own gas.
3. See 'N Say Farmer Says
Seriously, where would society be without this perennial favorite?! I mean it taught us that the cow goes "moo"!
4. Fisher-Price Record Player
This was probably the first sound system you ever owned — which of course you used to play your Read-Along Book records on.
5. Colorforms
These toys had no business being as fun as they were, essentially they were just reusable stickers. But maybe it was the perfectly cartoony design or the simplicity that had us coming back to play with it? Actually, scratch that, it was the addictive plastic smell that had us hooked.
6. Fisher-Price Medical Kit
Let's be honest, the only thing you really played with in this kit was the stethoscope. But, bonus, the kit did also make a good storage case to carry around other toys!
7. Gumby
During the 1980s Gumby had a huge revival, and this stretchy/posable figure was the perfect toy for little kids, as it was virtually indestructible. The one drawback was that, yes, you could eventually pull him apart and expose the wires that made him so darn posable, and maybe poke an eye out.
8. Mini Piano
This toy provided exactly 20 minutes of fun before you got bored and moved on to another toy — that is, unless your parents got tired of hearing the annoying beeps and took it away before you had a chance to finish playing with it.
9. Mickey Mouse Talking Phone
What kid didn't want to talk to Mickey Mouse or Donald Duck? Sure, they didn't say anything other then their name, but still you got a Disney character on the phone and it didn't cost your parents two dollars a minute — looking at you, He-Man.
11. Care Bears
The Care Bears ruled the '80s, and you want to know why? Because they were created by the greeting card company American Greetings, who pushed them on unsuspecting parents and grandparents, and guilted them into buying them as an "I love you" gift. Plus, their soft bodies and pastel colors made them irresistible to little kids.
12. Fisher-Price Family Farm
Aside from the Little People, the biggest seller here was the animals. Every little kid wanted to play with farm animals!
13. Fisher-Price Music Box Teaching Clock
This longtime favorite was supposed to teach you how to tell time, but in reality, it was just fun to wind it up and watch it turn from day to night.
14. E.T. Vinyl Doll
How big was E.T.? It was like Frozen x The Lion King plus a million. Seriously, it was huge, and of course had a natural appeal toward little kids, which made this vinyl doll (which felt so creepy to touch) a must have.
15. Poppin' Pals
This toy was probably more for babies than preschoolers, but its pop-up action made it lots of fun to play with.
16. Smurfs Play Camera
This toy was a double winner, not only was it a play camera (allowing you to feel oh so grown-up), but it was also Smurf-themed. SMURF-THEMED!
17. Monchhichis
Let's be honest, these were more creepy than cute, but that catchy jingle for the commercial made you want one of these, badly.
18. View-Master
Another perennial favorite that while simple provided hours of fun.
19. Fisher-Price Cash Register
I don't know if this was supposed to teach you how to use money, but I do know that the biggest appeal of this toy was popping that drawer open.
20. Tree Tots Family Tree House
This little house was perfectly designed to capture your imagination. It was a tree that with the push of a button (on the top of the tree) revealed a three-room house, that came complete with an elevator, staircase, swing, and garage.
21. Rub-A-Dub Dog
This toy pulled double duty. It was not only a bath toy, it was also a sponge (not a very good one, but still).
23. My Buddy and Kid Sister
You know why you wanted one of these dolls? Because of the jingle from the commercial, which probably still lingers in the deep recesses of your mind.
24. Fisher-Price Tape Recorder
This had one huge advantage over the Fisher-Price Record Player: IT COULD RECORD YOUR VOICE!
25. Snoopy Sno-cone Machine
No hot summer day was complete without this classic toy. Sure, it took all your preschool body strength to get three slivers of ice slushy, but all that hard work made it taste extra good.
26. Fisher-Price Magic Burner Play Stove Top Range
OK, so this probably gives you chills 'cause it looks like the hot plate you had in college, but back in the '80s this bad boy wasn't just a pretend stove, it was a pretend stove that also lit up red!
27. Sweet Pickles Bus
OK, so this wasn't a toy, but it was shaped like a bus and had a sticker that featured all the characters on it — which for fans of the book series made it an immediate toy (since no tie-in toy line was ever produced for it).
28. Little Boppers
These were basically like those dancing flowers that moved to music, expect they went one step further and actually moved around and danced. Yup, DANCED!
29. Speak & Spell
These really weren't designed for preschoolers, but all those buttons and the voice action made them an irresistible toy to little kids.
30. Popples
Popples was one of the only toys that was perfect for both playing with and napping with.
31. Fisher-Price Parking Ramp Service Center Parking Garage
This was the ultimate Little People play set (sorry, airport), mainly because you could race the cars down the ramp (with the Little People riding them), but the crank elevator also had its appeal.
- The Trump administration wants to send immigrants caught at the southern US border to Mexico even if they're not from the country.
- Several states will keep enforcing bans against transgender discrimination in schools after Trump withdrew guidelines protecting students.
- Police arrested at least 10 people on Wednesday as they began clearing out protester camps at the Dakota Access Pipeline site.
- And people with disabilities are having their photos stolen and Facebook isn't helping them.

subset: commoncrawl


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- 1[intransitive, transitive] to hit something with a hammer I could hear somebody hammering next door. hammer something (in/into/onto something) She hammered the nail into the wall. hammer something + adj. He was hammering the sheet of copper flat. hit many times
- 2[intransitive, transitive] to hit something hard many times, especially so that it makes a loud noise synonym pound Someone was hammering at the door. Hail was hammering down onto the roof. (figurative) I was so scared my heart was hammering (= beating very fast) in my chest. hammer something He hammered the door with his fists. Synonymsbeatbatter pound lash hammerThese words all mean to hit somebody/something many times, especially hard.beat to hit somebody/something a lot of times, especially very hard:Someone was beating at the door. A young man was found beaten to death last night. At that time, children were often beaten for quite minor offences (= as a punishment).batter to hit somebody/something hard a lot of times, especially in way that causes serious injury or damage:He had been badly battered around the head and face. Severe winds have been battering the coast.pound to hit somebody/something hard a lot of times, especially in a way that makes a lot of noise:Heavy rain pounded on the roof.lash to hit somebody/something with a lot of force:The rain lashed at the window. The subject of lash is often rain, wind, hail, sea or waves.hammer to hit somebody/something hard a lot of times, in a way that is noisy or violent:He hammered the door with his fists.pound or hammer? There is not much difference in meaning between these two, but to pound is sometimes a steadier action. To hammer can be more violent and it is often used figuratively.Patterns to beat/batter/pound/lash/hammer somebody/something with something to beat/batter/pound/lash/hammer against something to beat/batter/pound/hammer on something to beat/batter/hammer something down the rain/wind/sea beats/batters/pounds/lashes (at) something kick/hit ball
- 3[transitive] hammer something (+ adv./prep.) (informal) to kick or hit a ball very hard He hammered the ball into the net. defeat easily
- 4[transitive] hammer somebody (informal) to defeat somebody very easily Our team was hammered 5–1. Word Origin Old English hamor, hamer, of Germanic origin: related to Dutch hamer, German Hammer, and Old Norse hamarr
hit with toolverbjump to other results
BrE BrE//ˈhæmə(r)//; NAmE NAmE//ˈhæmər//Verb Forms present simple I / you / we / they hammer
BrE BrE//ˈhæmə(r)//; NAmE NAmE//ˈhæmər//he / she / it hammers
BrE BrE//ˈhæməz//; NAmE NAmE//ˈhæmərz//past simple hammered
BrE BrE//ˈhæməd//; NAmE NAmE//ˈhæmərd//past participle hammered
BrE BrE//ˈhæməd//; NAmE NAmE//ˈhæmərd//-ing form hammering
BrE BrE//ˈhæmərɪŋ//; NAmE NAmE//ˈhæmərɪŋ//
Check pronunciation: hammer


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Home Designer 2017 is now available! Whether you have a PC or Mac, a single license will run natively on either platform. Scroll down the page to learn more about the new features in Home Designer 2017.
Set Heights by Absolute or Relative Position. When editing objects, specify heights or offsets based on Absolute location in plan, or the object's relative position to other plan locations.
Live Color Elevations and Perspective to Layout. Send any view to layout page; views will retain scale, can reflect Render Techniques, and can be automatically updated in real-time.
Sun and Shadow Controls per Camera. Using the "Sun Rotates with the Camera" setting, you will always have a bright scene without being in shadow.
Cross-Section Slider with Multiple Cutting Planes. Choose up to six cutting planes in the Cross-Section Slider view and adjust the cutting distance for each plane to create unique and detailed views.
Paint One Wall. Quickly change the material on the surface of a single wall in a room.
3D Mouse Support. Use a 3Dconnexion Mouse as your input device to efficiently navigate camera views.
3D Navigation Overhaul. As you navigate in 3D, the camera follows your movement.
Cabinet Face Item Locking. Lock editing on cabinet face items as you modify a cabinet to more easily control the manipulation of the cabinet front. Size of appliances inserted into cabinet fronts are given higher priority, preventing them from being resized less frequently as the cabinet's face items are modified.
Top and Bottom Hinged Cabinet Doors. Design cabinets with awning and hopper style doors.
Specify Cabinet Top Height. Quickly adjust wall cabinet heights to the same level using the floor to top control in the Cabinet Specification Dialog.
Cabinet Doors on Narrow Cabinets. Specify doors or drawers on cabinets narrower than 6".
Objects Bump at Casings. Move cabinets, furniture, or other objects and bump to stop their movement when encountering door and window casings.
Corner Windows. Bump windows together at wall corners and choose to include or remove the corner post at the sash joint to create a glass corner.
Export Entire User Library. Easily backup and transfer User Library at the top level of the User Library Tree.
Symbol Object Specification Editable from Library. Edit specifications like Height Off Floor and Label for unlocked library items and save with the object in the Library Browser.


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Oracle set to push hardware
Oracle, the world's largest supplier of business software, expects to accelerate hardware sales led by strong adoption of its high-performance 'engineered systems' line in Asia, especially by large companies on the mainland.
Chief executive Larry Ellison said global sales of Oracle's flagship Exadata database machine were forecast to reach about 3,000 units in the financial year to May 31, up from 1,000 units in the previous financial year.
'We think this product is just going to take off,' Ellison said at his company's OpenWorld conference in San Francisco. 'We certainly deliver the fastest computer for business that's ever been built.'
'The idea of engineering the hardware and software - all of it - so it works together gives us the opportunity to deliver better performance and lower cost, better ease of use, better security and better reliability across the board.'
He described iPhone and iPad maker Apple as an example of a company that was 'doing a pretty good job of designing hardware, software and online services that work together'.
Oracle's engineered systems line consists of pre-configured computer platforms - contained in steel cabinets roughly the size of big household refrigerators - that integrate software, servers, storage and networking systems to handle complex business workloads.
Following its purchase of computer maker Sun Microsystems for US$7.4 billion last year, Oracle stepped up development and marketing of its engineered systems line to create a new product segment in enterprise hardware technology. Oracle's first version of Exadata was co-developed with Hewlett-Packard and released in 2008.
In Asia, where Oracle has about 80,000 customers, Ellison pointed out that large companies such as China Mobile were providing 'interesting' examples of improved business performance as they deployed Exadata.
'We see our engineered systems being used to address cloud computing by telecommunications companies and outsourcing providers,' said Steve Au-yeung, the executive vice-president at Oracle Asia-Pacific.
Cloud computing enables companies to buy, lease and receive over the internet software, information and other shared information-technology resources, such as storage, that are remotely hosted in data centres. 'Cloud' refers to the internet, which is depicted in that form in computer network diagrams.
The other big-ticket engineered systems products that support users of Exadata are Exalogic and the newly launched Exalytics.
Adrian Jones, the senior vice-president for hardware sales at Oracle's Asia-Pacific and Japan operations, said the company was keen to expand its investments in marketing and hardware distribution coverage to the mainland's second-tier cities. 'That represents 21 per cent of the total available market for IT in China today. That's US$1 billion worth of IT spending,' Jones said.
On the mainland, the adoption of Oracle's engineered systems is being led by the telecommunications, manufacturing, financial services and public sectors.
Roger Li Hon-cheung, senior vice-president at Oracle Asia-Pacific's technology business unit, said China Mobile, China Unicom and China Telecom were among the biggest users of Exadata machines on the mainland.
Other prominent mainland users include giant household appliances maker Haier, aviation industry technology provider TravelSky, China Metallurgical Group Corp, Shenhua Group Corp and Huawei Technologies, the world's second-largest telecommunications equipment manufacturer.
'We've sold many systems to the Chinese government,' said Marcus Tsoi Hon-fai, the senior vice-president for Oracle's Fusion Middleware business in the Asia-Pacific.
Tsoi recalled that in the beginning, the mainland's information-technology purchasing policy strictly separated hardware and software acquisitions.
'So engineered systems were not on the list,' Tsoi said. 'As soon as they realised the value of the product, they changed the policy.'
Carter Lusher, the chief analyst for enterprise applications at market research firm Ovum, said: 'China is an especially good opportunity for Oracle because Chinese companies are growing rapidly and require infrastructure that can scale up in a relatively easy manner.'
In a report published in August, market research firm IDC said: 'Customers needing more IT horsepower - for example, to run complex data warehouse set-ups and heavy transactional applications - have adopted Oracle Exadata.'
Technology analyst firm Gartner projects worldwide data centre hardware spending to reach US$98.9 billion this year, up 12.7 per cent from US$87.8 billion last year. Data centre hardware spending includes servers, storage and networking equipment.
As of September 15, top-of-the line Exadata database machine X2 cost US$1.65 million. Its most prominent competitors are IBM's Power 795 server and HP's Superdome.
The merger with Sun has also helped Oracle to increase the number of its corporate customers worldwide to nearly 380,000 and global revenue to US$35.6 billion in its last fiscal year.
The amount, in US dollars, that Oracle spends every year on research and development
- Revenue was US$35.6b last fiscal year
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The fee for a class is a sliding scale: $30-50 for Bike Kitchen members or $80-100 for non-members. Enrolling in the class as a non-member also buys you a year-long membership at the Bike Kitchen. Class size is limited. If you do not currently have a membership you MUST select a ticket price that has "Non-Member" in the description for your first class, but you may purchase them at the membership price for all future classes.
Refund Policy: due to limited resources, no refunds or re-scheduling can be offered. If you are unable to attend a paid class, your new membership is still valid and you are welcome to visit us during regular shop hours and speak to our staff mechanics.
Classes cover installing and removing tubes and tires (i.e. fixing a flat), installing brake and shift cables, adjusting brakes and gears, chain maintenance, replacing spokes and truing wheels.
We offer classes that cover the installation, overhaul, adjustment and maintenance of the bicycle's bearing systems (headsets, bottom brackets and hubs), freewheels and cassettes.
Please contact our education coordinator if you're interested in one-on-one classes with a wheelbuilder.
You will need to bring your own rim, hub, spokes and spoke nipples. We strongly discourage the use of used spokes or rim for this class, but a used hub is okay. Your favorite shop can help determine the correct spoke length, or you can search online for "spoke calculator."


text:
> Grow Your Own
> Hose Pipes
This Draper Tools Garden Hose Kit 30 Metres with Spray Gun is an ideal starter kit and comes complete with all the fittings needed for watering you...more
Sorry this product has been discontinued
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text:
Dry Kabuli Chana
|Kabuli chana
|250 Gram
|Cumin powder
|1 Teaspoon
|Garam masala
|1⁄4 Teaspoon
|1 Tablespoon
|3⁄4 Teaspoon
|1 Large, chopped
|Turmeric powder
|1⁄4 Teaspoon
|Green chillies
|2 , slit in centre
|1⁄6 Teaspoon
|1⁄2 , cut into strips
|Lemon juice
|2 Teaspoon
|Coriander leaves chopped
|2 Teaspoon
Soak the kabuli channa overnight.
The next day boil the channa.
Brown the onion in the ghee and add the kabuli channa, three green chillies, ginger, cumin seed powder, salt, turmeric and a quarter cup of water (or more if necessary).
Cook for five or ten minutes on a very low fire till the channa becomes quite tender, and dry.
Sprinkle lemon juice, and garnish with green chillies and Coriander leaves.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 1222 Calories from Fat 272
% Daily Value*
Total Fat 32 g48.7%
Saturated Fat 11.2 g56%
Trans Fat 0 g
Cholesterol 40.2 mg13.4%
Sodium 1577.1 mg65.7%
Total Carbohydrates 191 g63.7%
Dietary Fiber 50.3 g201.2%
Sugars 42 g
Protein 53 g106.3%
Vitamin A 22.2% Vitamin C 150.6%
Calcium 39.4% Iron 103.5%
*Based on a 2000 Calorie diet


text:
Leek cafe raided by cake, flapjack and scone thieves
- 23 February 2014
- From the section Stoke & Staffordshire
A community centre has been raided by thieves who stole from its cafe while a jazz band was playing in the main area.
Carrot cake, Victoria sponge, fruitcake, cheese scones and flapjacks were taken from the kitchen area by the intruders at the Foxlowe Arts Centre in Leek, Staffordshire.
Manager Ian Wilson said: "The chef will have to work like a demon to replace the cakes before opening tomorrow.
"I've only been here for 15 weeks, it really takes the biscuit - literally."


text:
A cell surface interaction network of neural leucine-rich repeat receptors
- Christian Söllner1, 2Email author and
- Gavin J Wright1Email author
DOI: 10.1186/gb-2009-10-9-r99
© Söllner and Wright; licensee BioMed Central Ltd. 2009
Received: 9 June 2009
Accepted: 18 September 2009
Published: 18 September 2009
The vast number of precise intercellular connections within vertebrate nervous systems is only partly explained by the comparatively few known extracellular guidance cues. Large families of neural orphan receptor proteins have been identified and are likely to contribute to these recognition processes but due to the technical difficulty in identifying novel extracellular interactions of membrane-embedded proteins, their ligands remain unknown.
To identify novel neural recognition signals, we performed a large systematic protein interaction screen using an assay capable of detecting low affinity extracellular protein interactions between the ectodomains of 150 zebrafish receptor proteins containing leucine-rich-repeat and/or immunoglobulin superfamily domains. We screened 7,592 interactions to construct a network of 34 cell surface receptor-ligand pairs that included orphan receptor subfamilies such as the Lrrtms, Lrrns and Elfns but also novel ligands for known receptors such as Robos and Unc5b. A quantitative biochemical analysis of a subnetwork involving the Unc5b and three Flrt receptors revealed a surprising quantitative variation in receptor binding strengths. Paired spatiotemporal gene expression patterns revealed dynamic neural receptor recognition maps within the developing nervous system, providing biological support for the network and revealing likely functions.
This integrated interaction and expression network provides a rich source of novel neural recognition pathways and highlights the importance of quantitative systematic extracellular protein interaction screens to mechanistically explain neural wiring patterns.
Identifying the vast number of precise intercellular connections that ultimately account for higher cognitive functions in vertebrate nervous systems, and explaining how they develop, remains one of the main challenges facing neuroscience [1]. Receptor proteins displayed on the surface of neurons are known to relay extracellular recognition events to elicit appropriate cellular responses such as axon guidance, neuron migration and synapse formation, but in comparison to the complex cellular networks that they regulate, relatively few extracellular recognition receptor interactions have been identified [2, 3]. Comparative genome analysis and large-scale gene expression studies, however, reveal that vertebrates contain large families of neurally expressed receptor proteins that are expanded relative to invertebrates [4]. These genes are likely to account for the increased complexity of vertebrate nervous systems and two major families are the leucine-rich repeat (LRR) and extracellular immunoglobulin superfamily (IgSF). The neuronal roles of some proteins containing IgSF domains have been well documented (see [5] for a review) but the functions of LRR family members are less well characterized.
Despite this involvement in neurological diseases, very little is known about their function and especially their extracellular binding partners. Indeed, of the approximately 20 paralogous subfamilies of membrane-tethered vertebrate LRR-domain-containing receptors [7], extracellular binding partners have been identified for just five: the Lingo, Lrrc4, Flrt, Amigo and NgR subfamilies. One explanation for this disparity is that membrane-embedded receptor proteins are experimentally intractable: they are generally of low abundance and their amphipathic nature makes them difficult to solubilise since they usually contain both large hydrophilic glycans and at least one hydrophobic transmembrane region. Interactions between receptor proteins are also characterised by extremely low interaction strengths, often having half-lives of fractions of a second when measured in their monomeric state [23]. The fleeting nature of these interactions is necessary to permit facile independent motility of migrating cells or growth cones when many receptor proteins arrayed on apposing cell membranes interact. These properties, however, make identifying novel extracellular recognition events mediated through cell surface proteins technically challenging.
The aim of this study was to identify novel receptor interactions that are involved in neural cellular recognition events, focussing in particular on the LRR and also IgSF receptor families. Furthermore, by identifying when and where each gene of an interacting pair is expressed during neural development, we could construct dynamic maps of the neural intercellular recognition program. Using a recombinant protein library of 150 neural receptor ectodomains and a highly stringent interaction assay suitable to detect low affinity extracellular interactions, we identified extracellular binding partners for orphan receptor families - such as the Lrrtms, Lrrns and Elfns - and novel partners for well-characterised receptors, including Unc5b. Paired spatiotemporal gene expression patterns of all genes within the network revealed when and where these interactions might occur during neural development. This neuroreceptor interaction network with integrated gene expression data provides a useful resource to mechanistically explain how complex cellular neural networks develop.
A protein interaction network of leucine-rich repeat neuroreceptors
To identify extracellular receptor interactions involved in neural recognition processes, we initially focused on the zebrafish LRR family since they represent a large group of receptor proteins expressed in the nervous system, many of which are 'orphan' receptors. We first identified members of this family by performing a comprehensive bioinformatics search of the zebrafish genome. Orthologues for each of the known type I membrane-tethered and glycophosphatidylinositol-linked mammalian subfamilies [7] were identified and at least one representative was successfully cloned by RT-PCR, with the one exception of the Lrig subfamily (Figure 1a; see Additional data file 4 for a comprehensive list). In total, ectodomain expression constructs were made for 53 genes, which accounts for the vast majority (approximately 80%) of this class of LRR neuroreceptors in the zebrafish genome. To identify novel interactions, we used the AVEXIS (for AVidity-based EXtracellular Interaction Screen) assay developed in our laboratory, which is able to detect very low affinity extracellular interactions (t1/2 ≤ 0.1 s) and can be scaled to screen thousands of binding events with a very low false positive rate [24]. This assay requires that each ectodomain is expressed as a monomeric biotinylated bait as well as a multimerized, enzyme-tagged prey (Additional data file 1). In total, 49 baits and 52 preys were expressed at sufficient levels and were then normalized prior to screening [24]. The biotinylated monomers were arrayed onto streptavidin-coated microtitre plates, and binary interactions identified by probing these arrays with the prey ectodomains. A primary screen between the LRR receptors of 49 × 52 = 2,548 interaction tests was performed and all positive interactions were then re-tested in both bait-prey orientations in a validation screen using fresh protein preparations. Seventeen interactions between 12 proteins were identified and classified into two confidence categories (Figure 1b; see Materials and methods for full details).
LRR neuroreceptors have binding partners within the IgSF
Since the IgSF is a well documented receptor family for LRR domains [26, 27], we next systematically screened the LRR proteins against a large library of 97 bait ectodomains belonging to the zebrafish IgSF (see Additional data file 5 for a comprehensive list). In total, 52 × 97 = 5,044 interactions were screened and positive interactions were subsequently retested using independent protein preparations in both bait-prey orientations. A further 17 interactions involving nine IgSF proteins were added to our neuroreceptor interaction network and similarly placed into two confidence categories (Figure 1c).
All interactions within the LRR-IgSF network except one [28] were previously unknown. The systematic nature of the screen revealed novel extracellular interactions for well described axon guidance receptors. For example, we identified novel LRR-domain-containing transmembrane ligands for the receptors Robo2 and 3, which we have shown bind to zebrafish Slit proteins (see Materials and methods) demonstrating that they were functionally active. Robo2 interacted with Lrrc24 and Lrrtm1, and Robo3 with Elfn1, suggesting that the Robo receptors are able to respond to local membrane-tethered signals in addition to secreted ligands such as Slit. Similarly, Unc5b, a known receptor for Netrin [29, 30], interacted with three out of the four Flrt-family homologs [31] (Figure 2). Other IgSF-LRR receptor interactions were found for the Lrrtm1 protein, which interacted with three out of four fibroblast growth factor receptor homologs in the screen (Fgfr4, Fgfrl1a and Fgfrl1b), and novel binding partners for both the axon guidance receptor Boc, and the myelin-associated glycoprotein Mag.
Interaction strengths between related neuroreceptors quantitatively vary
Paired receptor gene expression patterns reveal dynamic cellular neural recognition maps
The binding network of IgSF and LRR receptors (Figure 2) is a static representation of possible extracellular protein interactions and does not reflect the spatial and temporal ordering of recognition events used in the developing nervous system. To reveal when and where these binding events might occur, we determined the expression patterns of all the receptor genes within the network at four stages of zebrafish embryonic development (Additional data file 7 and see Materials and methods for details of an online database of paired stage and orientation-matched images) using mostly two-color fluorescent in situ hybridization to directly compare the expression of each gene encoding an interacting receptor pair within the same embryo.
This study represents the first step towards mapping an extracellular interaction network between neural receptor proteins, a resource that will be necessary to understand the intercellular recognition processes that ultimately underlie brain development and function. The importance of understanding these processes is becoming increasingly apparent as neurological disorders are more frequently being viewed as a product of abnormal brain development [34]. Significantly, we have described here binding partners for three orphan LRR receptor subfamilies, including the Lrrtms, which have been implicated in neurological diseases, including schizophrenia. While the LRR and IgSF are both large families of neurally expressed receptors, there are several other families of cell surface proteins that contribute to neural recognition processes. A comprehensive extracellular network of interactions within the developing nervous system will require the addition of these protein families to our protein library. Crucially, however, we have shown that the systematic screening approach using the AVEXIS method has the scalability and sensitivity to detect transient interactions that are not generally detected by other high throughput protein binding assays. Beyond identifying extracellular binding partners for orphan receptor families, this systematic unbiased method can identify additional binding partners for receptors that already have identified ligands.
Currently, our protein library contains approximately 80% of the zebrafish neural LRR receptors, providing a high density coverage for this family of receptors, which are known to be important for synaptic target selection [35]. We have shown that LRR receptor proteins are able to form both homophilic and heterophilic interactions within the family but also interact with receptors from the IgSF. Despite this large scale approach, we did not identify binding partners for all LRR subfamilies; indeed, both the Slitrk and Lrrc3 subfamilies still have no documented extracellular binding partner. LRR receptors are also known to bind other protein families such as the Netrin-G [14] and tumor necrosis factor-receptor family [36] and the future inclusion of these receptor families into our interaction screens is likely to reveal further binding partners for these subfamilies.
The AVEXIS assay was developed and implemented at a high stringency threshold to effectively eliminate false positives so as to produce high-quality datasets [24]. Using this stringency, approximately 0.5% of unique interactions screened - calculated using just one bait-prey orientation - are positive. Although difficult to directly compare due to the ascertainment biases inherent in selecting proteins restricted to a particular subcellular localization (such as the plasma membrane) or screening within protein families previously demonstrated to interact, this interaction frequency lies between large-scale binary yeast-two-hybrid assays (approximately 0.01%) [37] and the LUMIER assay (approximately 8%) [38]. The paucity of zebrafish protein interaction data makes a false negative rate difficult to assess, but by using the closest mammalian orthologue, the main class of false negatives comprised homophilic interactions. This is most likely due to prey-prey associations [24], although it should be noted that AVEXIS is able to detect some homophilic interactions and further work is required to determine the biochemical and/or structural reasons for this difference. A complementary scalable assay dedicated to identifying homophilic receptor interactions has been developed and could be used to specifically detect this class of interactions [39]. AVEXIS may also not be generally suitable to detect interactions between ectodomains that interact in cis to form co-receptor complexes since no interaction between NgR1 and Lingo1 ectodomains was detected [40]. During the preparation of this manuscript, an independent study reported the Flrt3-Unc5b interaction in Xenopus and demonstrated its role in cell adhesion processes during early embryogenesis [28].
The systematic nature of our screening approach revealed that many receptors have multiple binding partners with compatible expression patterns, raising the possibility of binding competition at the cell surface. While parameters such as abundance, local clustering and accessibility will also influence binding in vivo, the intrinsic binding affinity of a ligand for its receptor is important for resolving and measuring these effects. The finding that the three Flrt paralogs have different binding affinities for the Unc5b receptor, spanning at least an order of magnitude, was surprising and is likely to influence their ability to initiate signaling in vivo. Quantitative measurements of adhesion receptors in the immune system have shown that solution interaction strengths weaker than approximately 50 μM are unlikely to be high enough to support spontaneous interactions at physiological surface densities, highlighting the functional relevance of these measurements [41].
We have initially focused on two large families of neural receptor proteins - LRR and IgSF - as a starting point to begin a systematic approach to identify all extracellular recognition events required in the development of the vertebrate nervous system. In principle, this approach could be applied to other receptor families and secreted ligands. We anticipate that these networks of recognition receptors interpreted in the context of their corresponding gene expression patterns will provide a valuable new resource for neurobiology and will stimulate further research into the functional role of these interactions.
Materials and methods
Zebrafish husbandry
Zebrafish were maintained on a 14/10 hour light/dark cycle at 28.5°C according to UK Home Office and local institutional regulations, and staged according to Kimmel [42]. Embryos used for in situ hybridization were the progeny of a WIK/alb outcross; alb/alb embryos were used where endogenous pigment obscured staining signals.
Gene cloning and ectodomain library construction
The entire predicted extracellular and transmembrane regions of cell surface LRR-domain-containing genes were amplified by RT-PCR from mixed-stage zebrafish cDNA using oligonucleotides designed from automated gene predictions of the zebrafish genome [43]. PCR products were either cloned or used as further templates to amplify predicted ectodomains, which were ligated into a mammalian expression vector based on pTT3 [44]. The protein library was produced as previously described [24].
Interaction screen
Interactions were identified using the AVEXIS procedure as described [24]. Each plate contained both negative and positive controls as shown in Additional data file 1. Negative controls were the plate prey presented to the baits rat Cd4d3+4 (well H7), Cd200R (H8) and Cd200 (H9). Positive controls were Cd200R prey and Cd200 bait (H10) and the Cd200 bait diluted 1:10 (H11) and 1:100 (H12). Fifty-two LRR prey proteins were systematically screened against 49 LRR and 97 IgSF ectodomain bait proteins derived from membrane-bound receptors [24]. The vast majority of the LRRs and 28 of the IgSF proteins (indicated in Additional data file 5) were initially screened in both bait-prey orientations. Protein pairs that showed positive interactions in the first-pass screen were re-expressed and systematically re-screened in the same matrix-style manner as both baits and preys in an independent validation screen. Interactions that were positive in the first screen and could be detected in a reciprocal fashion were considered as high confidence interactions. Other interactions, such as those that were dependent upon the bait-prey orientation, were regarded as lower confidence interactions. Full details of the screening results are shown in Additional data file 6 and the protein interactions from this publication have been submitted to the International Molecular Exchange Consortium (IMEx) [45] through IntAct (pmid: 17145710) and assigned the identifier IM-11659. Expected interactions, including those between the zebrafish Robo and Slit orthologs, were detected in subsequent and ongoing interaction screens showing that the recombinant proteins are functionally active and full details are available at IntAct: Robo1-Slit1b, 2, 3 (EBI-2268920, EBI-2269164, EBI-2269173), Robo2-Slit2 (EBI-2269141) and Robo3-Slit1b, 2 (EBI-2269026, EBI-2268001).
Fluorescent bead binding
The extracellular regions of rat Cd200, Lrrn1, Vasn, Robo2, Lrrtm1, Unc5b and Flrt3 used in the AVEXIS screening were cloned into a pTT3-based expression vector to produce a chimeric construct that contained the transmembrane domains of the rat Cd200R and the green fluorescent protein in the cytoplasmic region. HEK293E cells were transfected with these constructs, harvested 2 to 3 days later, washed three times in phosphate-buffered saline/1% bovine serum albumin, vortexed and approximately 5 × 105 cells aliquoted into each well of a flat-bottomed 96-well microtitre plate. Interactions were then detected using a modified version of the fluorescent bead binding experiments described in [46]. Cells were then presented to biotinylated bait proteins immobilised around streptavidin-coated Nile Red fluorescent 0.4 to 0.6 μm microbeads (Spherotech Inc., Lake Forest, IL, USA) at a ratio of approximately 120 beads per cell. After incubating for an hour on ice the cells and beads were resuspended in 250 μl of phosphate-buffered saline/1% bovine serum albumin and analyzed for binding events using a BD LSR II flow cytometer and the data were analysed using FlowJo v7.5.3 software (Tree Star, Inc., Ashland, OR, USA).
Protein purification and BIAcore analysis
Protein purification and BIAcore analysis were performed as described [24]. Briefly, the ectodomain of Unc5b was produced in mammalian cells as a Cd4d3+4-6His-tagged protein and purified on a 1 ml His-Trap column (GE Healthcare, Amersham, Bucks, UK). Protein aggregates, which are known to influence kinetic experiments, were removed by gel filtration using a 125 ml Superose6 column prior to BIAcore analysis. The indicated amounts of the Flrt-Cd4d3+4-bio baits were immobilized onto a streptavidin-coated sensor chip and approximate molar equivalents of Cd4d3+4-bio were used as a reference. All binding studies were performed in HBS-EP buffer (GE Healthcare, Amersham, Bucks, UK) at zebrafish physiological temperature (28°C). Flow rates of 100 μl min-1 were used for kinetic studies to minimize rebinding effects and data were collected at the maximum rate of 10 Hz. Equilibrium dissociation and both on and off rate constants were calculated using the appropriate fitting model in the BIAevaluation software.
In situhybridization
Fluorescent two-color wholemount in situ hybridizations were essentially carried out as described [47]. RNA probes were prepared from a template amplified from the protein expression constructs encoding the entire ectodomain fragments. To facilitate comparison, single color images of the gene expression patterns at several stages of development were stage and orientation-matched and are presented in an online database at [48]. Expression data are also publicly available at [49].
Fluorescently labeled zebrafish embryos were mounted in Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA) and images were captured either on a Leica SP5 confocal microscope or a Zeiss Axioplan2 compound microscope fitted with a Volocity OptiGrid structured light device (Improvision, Coventry, UK).
Additional data files
The following additional data are available with the online version of this paper: a figure showing an outline of the AVEXIS procedure (Additional data file 1); a figure showing validation of interactions using a fluorescent bead-based assay (Additional data file 2); a figure showing that interacting neuroreceptors display both complementary and overlapping expression patterns in the developing brain (Additional data file 3); a table listing the zebrafish LRR genes cloned and used to produce recombinant ectodomains (Additional data file 4); a table listing the 97 zebrafish IgSF ectodomain baits (Additional data file 5); a table classifying the neuroreceptor interactions using AVEXIS (Additional data file 6); a table listing the spatiotemporal expression of each gene within the interaction network (Additional data file 7).
avidity-based extracellular interaction screen
- IgSF:
immunoglobulin superfamily
leucine-rich repeat.
We thank Bernard and Christine Thisse for high throughput in situ analysis; Jim Stalker for the online database; Madushi Wanaguru for help with bead binding experiments; and Seth Grant, Elisabeth Busch-Nentwich and members of the laboratory for comments on the manuscript. Our work was supported by the Wellcome Trust (grant number 077108/Z/05/Z) and both a Marie Curie and Sanger postdoctoral fellowships to CS. None of the funding bodies had any influence in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Authors' Affiliations
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MICR Code of UNION BANK OF INDIA (UBI) Nanpura Surat branch - Surat - NANPURA SURAT MICR Code
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MICR Code Details and Nanpura Surat Branch Information
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Union Bank Of India
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Branch :
Nanpura Surat
IFSC Code :
Click here for IFSC Code of UNION BANK OF INDIA (UBI) - NANPURA SURAT Branch
MICR Code :
Branch Code :
536415 (Last 6 Characters of the IFSC Code)
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Address :
Karani Building, Opp. Police Post, Main Road,surat, Nanpura, Gujarat,pin - 395 001.
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Nanpura Surat branch information
Indian Financial System Code, Magnetic Ink Character Recognition, SWIFT Code, Address and Contact number of UNION BANK OF INDIA (UBI) - Nanpura Surat branch. Branch office / ATM location : Karani Building, Opp. Police Post, Main Road,surat, Nanpura, Gujarat,pin - 395 001.
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If "zero-day exploit" hadn't previously been a part of your vocabulary, 2016 is the year to make the term stick. Cisco's annual report on the state of cyber security indicated a surge in the sophisticated hacks that fall under the zero-day heading. And that was before a number of recent events drew widespread concern from the cybersecurity field and the ...Read More »
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Social Networking to Play a Key Role in Modern Healthcare Delivery, According to IDC Health Insights Report
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As Western European healthcare providers continue to face challenges, the need for a new healthcare delivery model has become a hot topic of discussion. According to a recent report from IDC Health Insights, social networking will play a major role in this new delivery model. The report, Cooperation, Communication, Coordination: Three Pillars of Collaborative Teamwork (IDC Health Insights #HIOH51U, April 2012), describes the forces of change in the context of the transformation of the healthcare delivery model and explores the role of mobile technology in supporting collaborative teamwork.
Faced with cost constraints, aging populations, a higher incidence of chronic disease, staff shortages, regulatory pressures, and the need for improved quality of care and patient safety, healthcare providers are increasingly looking at integrated service delivery models to improve quality and safety through effective communication and standardized protocols.
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- Cooperation through collective, concurrent activities or efforts is needed to achieve a common goal
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- Mobile technologies will play a key role in collaborative care throughout the entire care life cycle
"Teamwork and collaboration are often used synonymously, but they are not the same," said Jan Duffy, EMEA research director, IDC Health Insights. "Effective collaboration may be viewed as either a process which affects the results of teamwork - enhanced patient care and provider satisfaction - or an outcome in and of itself. Whether or not it is an ingredient or a byproduct of teamwork, it is certain that the seamless provision of quality patient care today and in the future depends on inter-professional collaboration."
For more information about IDC Health Insights, or to arrange a one-on-one briefing with an IDC Health Insights analyst, please contact Kanupriya at +44 20898 77111 or email@example.com. Reports are available to qualified members of the media.
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Tommy Thayer Net Worth: Tommy Thayer is an American musician and songwriter who has a net worth of $10 million. Thayer is best known for being the lead guitarist for Kiss, a much popular hard rock band in America, as well as the former lead guitarist for the glam metal band Black 'n Blue. Born Thomas Cunningham Thayer on November 7, 1960 in Porttland, Oregon, Thayer's passion for music, especially the early 1970s hard rock, made him pick up electric guitar at age 13. After playing in a handful local bands, he decided to make a group of his own, Black 'n Blue, in 1981. Ever since its formation, the band has seen the release of 10 studio albums, enjoying worldwide fame and collaborating with notable musicians in the process. However, Tommy Thayer started to work with Kiss's Gene Simmons and Paul Stanley, especially after they asked him to work part-time on their forthcoming book Kisstor in 1994. This led to other projects in near future and eventually Thayer got a full-time role with Kiss with whom he worked on 8 studio albums. As an active supporter to community causes, Thayer sits on the Board of Trustees at Pacific University in Forest Grove, Oregon. He currently lives in the Los Angeles area together with his wife Amber.
Tommy Thayer Net Worth
Tommy Thayer
Date of Birth
Nov 7, 1960 (56 years old)
Guitarist, Songwriter, Musician
United States of America
More Rock Stars
All net worths are calculated by applying a proprietary algorithm. The results are then fact checked and confirmed by a team of editors. We scan all publicly available data and resources and work diligently to ensure that our numbers are the most accurate and up to date net worth data you will find anywhere on the internet.


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Bottle Gourd Sabji
|Bottle gourd
|500 Gram
|1 , grated
|2 , chopped
|1⁄2 Cup (8 tbs), grated
|6 Clove (30 gm), crushed
|2 Teaspoon
|1 1⁄2 Teaspoon, roasted
|Chili powder
|1 Teaspoon
|Turmeric powder
|3⁄4 Teaspoon
|1 1⁄2 Teaspoon
|1⁄2 Teaspoon
|Cumin seeds
|1⁄2 Teaspoon
|Cinnamon pieces
|3 Tablespoon
Peel bottle gourd and cut into big pieces.
Soak pieces in salted water.
Combine onion, coconut, grams, cumin seeds, cinnamon, pepper and powdered masalas.
Grind to a fine paste.
Add garlic.
Heat ghee in a pan and fry ground masalas.
Fry till ghee comes on the top; sprinkle some water if masala catches at the bottom.
Add vegetable, stir for a while.
Add one cup water.
Cover with a lid containing water and allow it to cook till bottle gourd is tender.
Add some warm water from lid if required.
Add tomatoes and sugar.
Cook for further five minutes.
Remove from fire.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 862 Calories from Fat 530
% Daily Value*
Total Fat 61 g93.9%
Saturated Fat 40 g200.1%
Trans Fat 0 g
Cholesterol 120.6 mg40.2%
Sodium 3028 mg126.2%
Total Carbohydrates 80 g26.6%
Dietary Fiber 15.8 g63.1%
Sugars 26.6 g
Protein 13 g26.1%
Vitamin A 73.3% Vitamin C 179%
Calcium 34% Iron 45.5%
*Based on a 2000 Calorie diet