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Springhill Suites by Marriott Hagerstown is located 1.1 mi (1.8 km) from Hagerstown Roundhouse Museum and within a 10-minute drive of Hagerstown Premium Outlets. This 3-star hotel has 104 guestrooms and offers an indoor pool, free in-room WiFi, and free self parking. Washington County Museum of Fine Arts and Maryland Theater are also nearby.
Free full breakfast is included with your stay.
The 104 air-conditioned rooms feature kitchenettes with refrigerators and microwaves. Guests can expect 32-inch LCD TVs with cable channels and pay movies, along with free WiFi and wired Internet. Bathrooms offer hair dryers and free toiletries, and beds sport premium bedding. Sofa beds, phones, and ironing boards are other standard amenities.
Property features
At Springhill Suites by Marriott Hagerstown, guests enjoy features like an indoor pool, a fitness center, and free WiFi in public areas. If you plan to drive, free parking is available. The front desk is staffed 24 hours a day to help with dry cleaning/laundry and securing valuables. Additional amenities include conference space, a business center, and coffee/tea in a common area.
- Free full breakfast
- Free WiFi and wired Internet in rooms and public areas
- Free parking
- Indoor pool
Springhill Suites by Marriott Hagerstown
Hotel Amenities
Hotel Amenities
Springhill Suites by Marriott Hagerstown features an indoor pool and a fitness center. The hotel offers a snack bar/deli. Guests can enjoy a complimentary breakfast. Public areas are equipped with complimentary high-speed wired and wireless Internet access.
This 3-star property offers access to a business center and a meeting room. This business-friendly hotel also offers coffee/tea in a common area, complimentary newspapers in the lobby, and a fireplace in the lobby. Onsite self parking is complimentary.
Springhill Suites by Marriott Hagerstown is a smoke-free property.
- 24-hour front desk
- Business center
- Free breakfast
- Coffee/tea in common areas
- Free newspapers in lobby
- Conference space
- Fireplace in lobby
- Dry cleaning/laundry service
- Fitness facilities
- Free WiFi
- Laundry facilities
- Meeting rooms
- One meeting room
- Number of floors - 6
- Total number of rooms - 104
- Safe-deposit box at front desk
- Free self parking
- Snack bar/deli
- Smoke-free property
- Indoor pool
- Television in common areas
- Free wired high-speed Internet
Family Friendly Amenities
- Family Hotel
- Indoor pool
- Free Wi-Fi
- Rollaway/extra beds available
- Kitchenette
- Snack bar/deli
- Laundry facilities
- Sofa bed
- Refrigerator
- Microwave
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi , Free wired high-speed Internet
Free self parking
Room Amenities
Springhill Suites by Marriott Hagerstown offers 104 air-conditioned accommodations with hair dryers and irons/ironing boards. Beds feature premium bedding. 32-inch LCD televisions come with cable channels and pay movies. Accommodations at this 3-star hotel have kitchenettes with refrigerators and microwaves. Bathrooms include shower/tub combinations and complimentary toiletries.
Guests can surf the web using the complimentary wired and wireless high-speed Internet access. Business-friendly amenities include desks and phones. Housekeeping is provided daily.
- Air conditioning
- In-room climate control (air conditioning)
- Free toiletries
- Cribs/infant beds available
- Desk
- Hair dryer
- Daily housekeeping
- Iron/ironing board
- Kitchenette
- Cable TV service
- Microwave
- Pay movies
- Premium bedding
- Refrigerator
- Rollaway/extra beds available
- Shower/tub combination
- Sofa bed
- Phone
- Free WiFi
- Free wired high-speed Internet
Where to Eat
Guests are offered a complimentary full breakfast.
Nearby Things to Do
Recreational amenities at the hotel include an indoor pool and a fitness center.
The recreational activities listed below are available either on site or nearby; fees may apply.
- Golfing nearby
- Downhill skiing nearby
- Snowboarding nearby
- Kayaking nearby
- Mountain biking nearby
- Bicycle rentals nearby
- Hiking/biking trails nearby
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
- Accessible bathroom
- Roll-in shower
- In-room accessibility
Hotel Policies
Check-in time starts at 3 PM
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Rollaway/extra beds are available.
- Cribs (infant beds) are available.
- Pets not allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Hotel Name
- Hagerstown Marriott
- Hagerstown Springhill Suites
- Marriott Hagerstown
- Springhill Suites Hagerstown
- Springhill Suites Marriott Condo Hagerstown
- Springhill Suites Marriott Hagerstown
- Springhill Suites Marriott Hagerstown Hotel


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is the philosophy and driving force behind our successful "hands on" approach to management. For more than 30 years, the founders of our company have been leaders in the field of Condominium Development and Asset Management. We have led the industry in developing and evolving a management process that benefits both the developer and homeowners.
As leaders we recognize that just like the homeowners themselves, every community has a unique personality. Through a combination of best practices and personal service, we bring the developer, the homeowners, and all others involved in the community together in a productive and compatible relationship allowing for the successful fruition of common goals.
The ongoing success and vitality of any community starts at the property's inception. As a developer, the decision you make early on in selecting a management company can positively influence the success of your project. We pride ourselves in becoming your business partner and working with you to develop and grow your asset.
Mission Statement
It is our commitment to every Homeowner, Board of Directors, Investor, Developer, and Client, to provide exemplary customer service, consistent and accurate reporting, continual loyalty and trustworthiness, timely response and follow through. To become an integral part of the "Community Team", enhancing their lifestyle experiences and together working towards the increasing of property Values. We do this through constant open communication, proactive management, dedications and professional commitment in all that we do.
Senior Staff
Click Here for more information.
Expert Accounting Department
Click Here for more information.
Certified Maintenance Professionals
Click Here for more information.
Emergency Response Team
Click here for more information

subset: commoncrawl


text:
Short Sleeve Mix Media Blouse
Product not available
Have questions?
Chat with us or call 1.888.282.6060.
HideDetails & Care
A light and flowy blouse with a high/low hem is fashioned with a woven front matched to a soft knit back.
- Approx. length from shoulder: front 25 1/2"; back 28".
- Cuffed sleeves.
- Rayon with rayon/spandex contrast; hand wash.
- By Pleione; made in the USA.
- Point of View.


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Levy and Citrus Counties
An impoundment of the Withlacoochee River, as part of the original Cross Florida Barge Canal project, this large lake is full of cypress trees, pennywort islands and sometimes hydrilla. Abundant cover and structure grow plenty of bluegill, redear sunfish, catfish, black crappie and largemouth bass. The deeper river channel is always good to locate and fish underwater bends. The rest of the lake is referred to as the "flats" or the backwaters.
Local contacts:Big Bass Village 352-447-3474, toll free 1-877-463-4742Lake Rousseau RV Park/Fishing Resort 352-795-6336, lakerousseaurvpark.comDunnellon Bait and Tackle 352-465-2755
TrophyCatch is FWC's citizen-science program that rewards anglers for documenting and releasing trophy bass 8 pounds or larger. The following TrophyCatch bass have been submitted from Lake Rousseau:
Lunker Club (8 – 9.9 pounds): 28
Trophy Club (10 – 12.9 pounds): 5
With cooler temperatures bass will be near vegetation and actively hunting for shad. For the best odds of connecting with a trophy bass, try trolling live golden shiners along vegetation. Spinners fished this way will also work well. Panfish are still biting. Try using grass shrimp, crickets, or red wigglers. Redear Sunfish can be more effectively targeted while fishing worms close to or on the bottom. Good numbers of catfish can still be caught by using baits fished on the bottom. Try using cut shad, shrimp, or a live bluegill (caught by legal methods). Black Crappie will become more active and can be caught by trolling using live minnows and beetle spins in deep water.
Big Bass are being seen more and more in the north eastern area of the lake closer to the river. Register online with FWC's TrophyCatch program and enter your trophy bass for a chance to win prizes. Entering fish is as simple as submitting a photo of the fish on a scale to the website above. The photo must show the entire fish and clearly show the weight on the scale.
FWC Facts:The Gulf sturgeon spawns 140 miles upstream from the mouth of the Suwannee River, one of the last pristine rivers with no dams to bar its path.
Learn More at AskFWC
Get FWC News Feeds
Copyright 1999-2017 State of Florida • Technical Help •
EEO/AA/ADA • Privacy Statement • Advertising Statement & Disclaimer • Site Map
Florida Fish and Wildlife Conservation Commission • Farris Bryant Building
620 S. Meridian St. • Tallahassee, FL
32399-1600 • (850) 488-4676
Pursuant to section 120.74, Florida Statutes, the Fish and Wildlife Conservation Commission has published its 2016 Agency Regulatory Plan.
Under Florida law, e-mail addresses are public records. If you do not want your e-mail address released in response to a public records request, do not send electronic mail to this entity. Instead, contact this office by phone or in writing..
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There will be free school immunizations, eye screenings, and blood-pressure, diabetes and cholesterol checks scheduled from 9 a.m. to 1 p.m. Saturday at the Ward 7 Citizens Club, 5006 La. 56, Chauvin. There will also be free school supplies distributed. There will be free food, games and door prizes. For information, call 851-7847.
Senior Center schedule: Bingo, 12:30 p.m. Thursday; transportation to grocery, bank and pharmacy, 12:30 p.m. Friday; ceramics, 8:30 a.m. Monday; poker-keno, 9:15 a.m. Tuesday; poker-keno, 9:15 a.m. July 28; lunch, 11 a.m. daily; and cards and billiards, 8:30 a.m. weekdays.
For information, call the center's manager, Arlene Levron, at 594-6741 or 594-8905.
Bingo schedule: Ward 7 Citizens Club's bingo is scheduled for 7:30 p.m. Thursday at its club home, 5006 La. 56, Chauvin.
Registration: The Terrebonne Parish Recreation Department is taking registration for Bantam football and cheerleading for children 7 to 13, and girl's volleyball for children 9 to 14. Registration began Monday at the Little Caillou Recreation Center, 215 Angel St., Chauvin. You can register from 4:30 to 8 p.m. Mondays through Thursdays at the gym. The registration fee is $20 and a birth certificate is needed. For information, call 594-2020.
Gym hours: The Little Caillou Recreation Center will have new summer hours for the gym. The recreation center is open from 1 to 8:30 p.m. Mondays through Thursdays, unless the center has a pre-scheduled activity during that time. For information, call the gym at 594-2020.
Library news: Activities for young adults are planned for the upcoming week at the Chauvin Branch Library, 5500 La. 56. A closing party for pre-kindergartners through sixth-graders who have completed the Summer Reading Club is scheduled for 11 a.m. Thursday. For information, call 594-9771.
Meetings: Members of the Chauvin Lions Club are scheduled to meet at 7 p.m. today at the club home, 145 La. 58, Chauvin.
Members of the Woodmen of the World are scheduled to meet for a business meeting at 6:30 p.m. Tuesday at the club's lodge, 206 La. 28, Chauvin.
Members of the St. Joseph Knights of Columbus Council Auxiliary No. 5013 are scheduled to meet at 7 p.m. Thursday at the club home, 201 La. 58, Chauvin. The rosary will be recited at 6:30 p.m. Bring a covered dish.
Birthdays: Kelly Lyons, Polly Scott, Bobby LeBeouf, James LeBoeuf, Ethan Molinere, July 21; Kent LeCompte, Deanna LeCompte, Whitney Price, Holly Rush, Gareth Henry, Christopher Verdin, July 22; Cindy Pierron, Emilie Blanchard, Rachel Willis, Debbie Falgout, Donna Domangue, Michelle Lyons, Joey Champagne, July 23; Malette Terrio, Issac Hendon, Jay Ryman, Jeanine Ledet, James LeBoeuf, Alena LeCompte, Cheryl Lirette, Melissa Hunter, July 24; Mark Karns, Courtney Neil, B.J. Ryman, Eddie Trahan, Juanita Naquin, Joy Luke, Lauren Christensen, Jacob Ledet, Blaise Chauvin, Tonya Roberts, July 25; Jeremy Authement, Chloris Caillouet, Eunice Pellegrin, Ivy Price Sr., Shane Hebert, Trever Pellegrin, Christian Authement, Gus Marie Sr., July 26; Mary Lee Andrews, O'Neil Theriot, Lonnie Thibodaux and Maybelle Hebert, July 27.
Anniversaries: Kenneth and Pat LeCompte, July 22; Elsie and Delvin Henry, July 23; Melissa and Gary Howard, Sheila and Cyrus Pitre, Emilie and Timmy Blanchard, Cheryl and Ray Lirette, July 24; Brenda and Brent Theriot, Joyce and Williard Thibodaux, Donna and Dennis Domangue, July 25; Lena and Conrad Thibodaux, July 26; Eddie and Jo Ann Neil, Lisa and Irby Bergeron, and Sue and Keith Adams, July 27.
CHAUVIN: Immunizations are free Saturday
There will be free school immunizations, eye screenings, and blood-pressure, diabetes and cholesterol checks scheduled from 9 a.m. to 1 p.m. Saturday at the Ward 7 Citizens Club, 5006 La. 56, Chauvin. There will also be free school supplies distributed. There will be free food, games and door prizes. For information, call 851-7847.


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User Agent: Mozilla/5.0 (Windows NT 6.0; rv:8.0) Gecko/20100101 Firefox/8.0
Build ID: 20111104165243
Steps to reproduce:
This is a follow up from Bug 689834
Created attachment 578362 [details] [diff] [review]
Support for defaultMuted attribute v1
Unsure whether i could say it fully depended on the 689834 as NS_IMPL_BOOL_ATTR needed it for resolving the muted attribute passed to it.
(In reply to andrew (:drexler) from comment #2)
> Unsure whether i could say it fully depended on the 689834 as
> NS_IMPL_BOOL_ATTR needed it for resolving the muted attribute passed to it.
Right, this patch depends on that patch because you need the muted atom. I've readded it. I thought it'd be simpler to split this out from bug 689834, but I was probably wrong as the trickier part is actually that bug and splitting things may have added to the confusion, sorry.
+ Support for defaultMuted attribute
Please include the bug number in the commit message.
+[scriptable, uuid(dcf812f5-373b-4Fc1-9a60-59078af33bf7)]
Why's 'F' in caps?
(In reply to Matthew Gregan [:kinetik] from comment #3)
> +[scriptable, uuid(dcf812f5-373b-4Fc1-9a60-59078af33bf7)]
> Why's 'F' in caps?
Oh missed that. Visual Studio's GUID generator has it in caps so manually changed it. I will resubmit the patch with the changes.
Created attachment 578980 [details] [diff] [review]
Support for defaultMuted attribute v2
Can you please write a mochitest and add it to content/media/test? If you need a base, test_paused.html is a simple one to start from. The main things to test is that <audio> and <audio muted> start in the expected states, and that toggling defaultMuted does not affect muted (and vice versa).
Sure will do that.
Created attachment 579823 [details] [diff] [review]
Patch v3
defaultMuted attribute implementation and associated test.
Looks good, thanks. I'll hang off on r+ing until we've got bug 689834 sorted out.
+ v1.defaultMuted = true;
+ a1.defaultMuted = true;
+ ok(v1.defaultMuted, "v1.defaultMuted should be true");
+ ok(a1.defaultMuted, "a1.defaultMuted should be true");
Can you also add a hasAttribute("muted") test here. That'll prove that the defaultMuted DOM attribute is correctly mapped to the muted content attribute. Also, check that the muted DOM attribute remains false.
+ v2.defaultMuted = false;
+ a2.defaultMuted = false;
+ ok(!v2.defaultMuted, "v2.defaultMuted should be false");
+ ok(v2.muted, "v2.muted should be true");
+ ok(!a2.defaultMuted, "a2.defaultMuted should be false");
+ ok(a2.muted, "a2.muted should be true");
And for this, a !hasAttribute("muted").
Created attachment 581535 [details] [diff] [review]
Patch v4
Added hasAttribute() checks to test_defaultMuted.html
Created attachment 581844 [details] [diff] [review]
Patch v5
Added extra checks for state of muted attribute in test_defaultMuted.html per bz's suggestion in comment 35 of bug 689834.
Comment on attachment 581844 [details] [diff] [review]
Patch v5
Also added the "muted" attribute on:


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At KOA, we love having fun and inviting new folks to join us online as well as at our campgrounds. Share your camping experiences, stories, photos and videos!
Each month, direct to your email inbox, we'll send the best camping news, tips, recipes and more, to enhance the camping lifestyle that you love so much.
Learn More
These tips from a seasoned family camper will ensure your next (or first) c...
Explore five new reasons to stay at a KOA campground and consider camping f...
Unsure about the different classes of RVs? Our quick guide is the perfect r...
Plan the perfect trip from home or on the road with the official KOA app. Learn More
Updated 2/19/17:Santa Cruz/Monterey Bay KOA is preparing for our next storm. Please call for any updates. For our recreational vehicle visitors please make sure you are stocked up on fresh water, propane, generator, battery power and food.Please check road conditions and for outages:https://m.pge.com/?WT.pgeac=Home_Outages#outageshttp://www.dot.ca.gov/cgi-bin/roads.cgihttp://www.sccroadclosure.org/
Open All Year
Reserve: 800-562-7701
Info: 831-722-0551
1186 San Andreas Road
Watsonville, CA 95076
We are located 11 miles South of Santa Cruz.
Take Hwy 1 South to San Andreas Rd Exit 432 and go W 3.2 miles on San Andreas Rd.We will be on your left. Look for the Yellow KOA Sign!
**If you are traveling on Hwy 1 North-DO NOT TAKE BUENA VISTA EXIT.And despite what GPS, Google maps or other services may inform you, DO NOT TURN ON PEACEFUL VALLEY DR.**
Take Hwy 1 North, take exit 425 onto W Riverside Dr, take a left on W Riverside Dr toward Lee Rd.In .2 miles, take a right on Lee RdIn .2 miles, take a left on W Beach StIn 1.4 miles, take a right on San Andreas RdIn 4.2 miles, destination will be on your right - look for the Yellow KOA Sign!
Camper Rating 4.5 out of 5
Based on 1000 Reviews
Download PDF
Whether you're looking for a base camp to explore the area or lots of fun activities right on the campground, KOA Holidays are great getaways. There's plenty to do, with amenities and services to match the way you camp. KOA Holidays even have our new Deluxe Cabins with full baths and kitchens! Take a hike, see the local attractions or just relax. It's your holiday!
Do not include the length of your tow or pull vehicle.


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ZouaveDouble click on any word to get a popup explanation of the word and sample sentences Dictionary | Wikipedia | Synonyms | News
Zouave Meaning and Definition in Dictionary
Would you like to add your own explaination to this word 'Zouave'?
Wikipedia Meaning and Definition on 'Zouave'
- Zouave: The Zouaves were a class of light infantry regiments of the French Army serving between 1830 and 1962 and linked to French North Africa, as well as some
- Papal Zouaves: The Papal Zouaves (Italian: Zuavi Pontifici) were an infantry force formed in defence of the Papal States. The Zouaves evolved out of a unit formed by
- USS Zouave (1861): USS Zouave (1861) was a steamer acquired by the Union Navy during the American Civil War. She was needed by the Navy to be part of the fleet of ships to
- The Zouave: The Zouave is the subject of two paintings made by Vincent van Gogh in Arles. Van Gogh was excited to have a model for a portrait, and painted two of the
- Zouave jacket: A Zouave jacket is a short open fronted jacket with long sleeves, similar to that historically worn by Zouaves. It was a popular women's fashion in the
- 8th Zouaves Regiment: The 8th Zouaves Regiment (French: 8e Régiment de Zouaves , 8e R.Z) was a French infantry unit of the French Army. Created in 1914, the unit was designated
- Zouaves of Death: sergeant in the French Zouaves. He based his formation on the French Zouaves, in which he had served during the Crimean War. The Zouaves of Death were highly


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2 XHTML Pages to DIV
This project was awarded to rahulsandleya for $30 USD.Get free quotes for a project like this
Project Budget$30 - $250 USD
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2 XHTML Pages to DIV
It is designed with tables
- No CSS
-clean W3C errors
On your website you say this:
- 1-3 days turnaround
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- Web 2.0 valid code
- SEO optimized
MUST MUST DELIVER IN 6 -8 hrs
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- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
Published: March 11, 2014 - 01:14 PM
COLUMBUS: School districts across Ohio would get four extra calamity days because of this winter's extreme weather under a compromise struck by state legislators Tuesday.
Under the measure headed to floor votes in both legislative chambers today, districts that have exhausted their five allotted calamity days could use the additional days only after holding school on four other scheduled days off, such as on holidays or during vacations.
The measure also allows districts to continue to make up missed days through 30-minute increments tacked onto regularly scheduled school days.
They also have the option of using work-at-home "blizzard bags" and online lessons to help students catch up on missed work.
Snow, ice and bone-chilling temperatures have led many districts across Ohio to exhaust all their calamity days, most commonly called "snow days," on which schools can close without making up the lost instructional time.
Republican Gov. John Kasich backed adding extra snow days on a one-time basis.
Many public school districts across Ohio have canceled classes for nine or more days this year.
Some Guernsey County schools in the eastern part of the state have topped 17 such days.
Tuesday's decision followed an impasse last month over two competing versions of the bill.
The conference committee removed a divisive provision added in the Senate that would have tied extra calamity days to professional development training for teachers. It also expanded the number of proposed calamity days from three to four.
Each school district adopts a contingency plan each year that includes adding at least five whole days to the school year if needed to make up any days missed beyond the excused calamity days.
The bill allows districts to revise those plans, which were submitted last September.
Under the compromise bill, districts may also excuse graduating seniors for any makeup days that occur after commencement ceremonies.
The legislation also extends the deadline for processing state-required testing of fourth- through eighth-graders to June 30.


text:
Education in the United States is mainly provided by the public sector, with control and funding coming from three levels: state, local, and federal, in that order. The common requirements to study at a higher education level in United States will include your admissions essay (also known as the statement of purpose or personal statement), transcript of records, recommendation/reference letters, language tests
Evansville is the third-largest city in the state of Indiana. It is known to be a business hub for a trade area made up of the states of Kentucky, Illinois and indiana.
Top Bachelor Degrees in Health Sciences in Evansville in USA 2017
The U.S. health care system changes quickly, and you can stay ahead of the curve by completing a bachelor's degree in health services at USI. Employment in the medical field is expected to grow faster than the average for all occupations as the industry continues to expand and diversify. [+]


text:
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Are you a trivia whiz? Test your knowledge on our daily quiz.
Got a good quiz question? Send your questions to firstname.lastname@example.com
Share your scores on Twitter using #stuffquiz or #nzstuffquiz.
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Scientific studies reveal the best age to get married
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5 common mistakes to avoid when washing your sheets
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Something's afoot with this gym-goer's feet
A cultural celebration acknowledging Pasifika heritage holds third event in Taranaki
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Architects' own home is the skinny new house on the block
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Autumn is perfect for alfresco living with an outdoor fireplace
A man has created a 'feminine lipstick' as an alternative to pads tampons and cups
Hokonui Runanga elder wants a clean Mataura river for grandchildren
Man dies after loader accident at Bluff port
Invercargill City Library launches World War I letters website
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Meat company mergers could mean more dollars
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Online early childhood survey released
'She's a victim too', Otago crash victim says of tourist
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Tourist driver ordered to pay $35,000 over crash that left cyclist in coma
Man punched "multiple times'' in head in Queenstown assault


text:
Biogeochemical relationships between ultrafiltered dissolved organic matter and picoplankton activity in the Eastern Mediterranean Sea
Meador, Travis B.
Concept link
Gogou, Alexandra
Concept link
Spyres, Georgina
Concept link
Herndl, Gerhard J.
Concept link
Krasakopoulou, Evangelia
Concept link
Psarra, Stella
Concept link
Yokokawa, Taichi
Concept link
De Corte, Daniele
Concept link
Zervakis, Vassilis
Concept link
Repeta, Daniel J.
Concept link
MetadataShow full item record
Citable URIhttp://hdl.handle.net/1912/3221
As publishedhttps://doi.org/10.1016/j.dsr2.2010.02.015
KeywordDOM; Biogeochemical cycles; Ultrafiltration; AOU; Microbial loop
We targeted the warm, subsurface waters of the Eastern Mediterranean Sea (EMS) to investigate processes that are linked to the chemical composition and cycling of dissolved organic carbon (DOC) in seawater. The apparent respiration of semi-labile DOC accounted for 27 ± 18% of oxygen consumption in EMS mesopelagic and bathypelagic waters; this value is higher than that observed in the bathypelagic open ocean, so the chemical signals that accompany remineralization of DOC may thus be more pronounced in this region. Ultrafiltered dissolved organic matter (UDOM) collected from four deep basins at depths ranging from 2 to 4350 m exhibited bulk chemical (1H-NMR) and molecular level (amino acid and monosaccharide) abundances, composition, and spatial distribution that were similar to previous reports, except for a sample collected in the deep waters of the N. Aegean Sea that had been isolated for over a decade. The amino acid component of UDOM was tightly correlated with apparent oxygen utilization and prokaryotic activity, indicating its relationship with remineralization processes that occur over a large range of timescales. Principal component analyses of relative mole percentages of monomers revealed that oxygen consumption and prokaryotic activity were correlated with variability in amino acid distributions but not well correlated with monosaccharide distributions. Taken together, this study elucidates key relationships between the chemical composition of DOM and heterotrophic metabolism.
Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Deep Sea Research Part II: Topical Studies in Oceanography 57 (2010): 1460-1477, doi:10.1016/j.dsr2.2010.02.015.
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Diversity and productivity of photosynthetic picoeukaryotes in biogeochemically distinct regions of the South East Pacific Ocean Rii, Yoshimi M.; Duhamel, Solange; Bidigare, Robert R.; Karl, David M.; Repeta, Daniel J.; Church, Matthew J. (John Wiley & Sons, 2016-01-08)Picophytoplankton, including photosynthetic picoeukaryotes (PPE) and unicellular cyanobacteria, are important contributors to plankton biomass and primary productivity. In this study, phytoplankton composition and rates ...
Coupled biogeochemical cycles : eutrophication and hypoxia in temperate estuaries and coastal marine ecosystems Howarth, Robert W.; Chan, Francis; Conley, Daniel J.; Garnier, Josette; Doney, Scott C.; Marino, Roxanne; Billen, Gilles (Ecological Society of America, 2011-02)Nutrient fluxes to coastal areas have risen in recent decades, leading to widespread hypoxia and other ecological damage, particularly from nitrogen (N). Several factors make N more limiting in estuaries and coastal waters ...
A framework to assess biogeochemical response to ecosystem disturbance using nutrient partitioning ratios Kranabetter, J. Marty; McLauchlan, Kendra K.; Enders, Sara K.; Fraterrigo, Jennifer M.; Higuera, Philip E.; Morris, Jesse L.; Rastetter, Edward B.; Barnes, Rebecca T.; Buma, Brian; Gavin, Daniel; Gerhart, Laci M.; Gillson, Lindsey; Hietz, Peter; Mack, Michelle C.; McNeil, Brenden; Perakis, Steven S. (2015-11)Disturbances affect almost all terrestrial ecosystems, but it has been difficult to identify general principles regarding these influences. To improve our understanding of the long-term consequences of disturbance on ...


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Women's Water Polo
California Baptist at UCLA
Sat., Jan 18
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Carlee Kapana Named MPSF Player of the Week
Bruin goalie surrendered just one goal and tallied 13 saves in win over No. 6 UC Irvine.
No. 3 UCLA Routs No. 6 UC Irvine in Home Opener
Three Bruins netted hat tricks in the 16-1 win.
Bruins Set to Open Home Slate with UC Irvine
The Bruins will host the Anteaters Friday at Spieker Aquatics Center.
UCLA Coasts to 13-2 Win Over Long Beach State
No. 3 Bruins will host alumni game Saturday at 2:00 p.m.
UCLA to Visit Long Beach State, Host Alumni Game
No. 3 Bruins will look to improve upon 6-0 mark.
Musselman Named MPSF Newcomer of the Week
The award is the second consecutive for the freshman to begin the season.


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You just started the world of promotional blog giveaways..... You get someone to give away a really cool product on your blog........... Now you just need someone to enter the giveaway!!
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After a bit of a hiatus, Read This Next is back, with a book of truly massive proportions. This week's title is Parallel Stories by Hungarian author Peter Nadas, which is translated by Imre Goldstein and just out from FSG.
It's impossible to mention this book without talking about its size and scope. The 1,133-page novel opens in 1989 with a university student in Berlin discovering a corpse during his morning run. From there, the novel stretches back to 1939 to relate a series of interconnected stories focusing on three "unusual" men: Hans von Wolkenstein, whose mother is linked to "secrets of fascist-Nazi collaboration," Agost Lippay Lehr, whose father worked for Hungary's political regimes, and Andras Rott, "who has his own dark record of mysterious activities abroad."
Nadas spent 18 years writing this book (or series of books—I've always heard of this referred to as a trilogy), yet, according to editor Elizabeth Sifton, this is a very tight, very well-constructed novel.
And Tim Nassau, who reviewed this for us, claims it's one of the best books he's ever read, one that he would recommend indiscriminately. Here's another bit of his review:
Most of the books I have reviewed for this site were only reviewed in one or two other places: small journals, literary blogs, a paragraph in Publishers Weekly, perhaps . . . This is, of course, the norm for literature in translation, and the discrepancy between the quality and coverage of these books has been bemoaned enough that I do not need to revisit it here. Every so often, however, a new book comes out that is big enough and important enough for its translation to be an event, and everyone takes notice. [. . .]
Which is why I do not intend to review Parallel Lives. Enough people will do so that several will surely do a better job than I could; Jonathan Lethem reviewed 2666 for the New York Times, and, to be honest, I find such a high level of competition intimidating. The case might be different if I hated the novel, if I believed that all the hype was just a ploy to move copies, but I do not believe these things. If you have come here to know, simply, if you should read this book, then the answer is yes. It is one of the best novels I have read, and I recommend it indiscriminately. Here is post-war Eastern Europe: an encyclopedia of people's lives as exhaustive in detail as it is ambitious in scale, an unflinchingly honest depiction of political and personal perversions. Yes, the characters are the products of Nádas's imagination, but the way he describes their emotions and motivations reveals such an uncommonly deep and sensitive understanding of what forces constitute any person that the reader cannot help but feel he is gazing at his own soul.
So instead of talking about the plot of the book, etc., Tim spends most of the review praising Imre Goldstein's translation. You can read the full review by clicking here.
And after reading that, I'm sure you'll want to read an excerpt from the book itself, which you can do by clicking here. (It's fitting that this, the longest book we've featured in Read This Next also has the longest sample.)
In addition, you can also click here to hear Elizabeth Sifton's comments on the book. It's cool to hear her talk about this, and she manages to make this sound even more interesting.
Finally, FSG published this interview with Nadas, which is also worth checking out.
Prose translators will likely disagree, but I believe translating poetry requires a significant level of talent, a commitment to the text, and near mania, all of which suggests that the undertaking is the greatest possible challenge. The task is to. . .
The biggest issues with books like The Subsidiary often have to do with their underpinnings—when we learn that Georges Perec wrote La Disparition without once using the letter E, we are impressed. Imagine such a task! It takes a high. . .
Following The Infatuations, Javier Marías's latest novel seems, like those that have preceded it, an experiment to test fiction's capacity to mesmerize with sombre-sexy atmospheres and ruminative elongated sentences stretched across windowless walls of paragraphs. Thus Bad Begins offers his. . .
Death by Water, Kenzaburo Oe's latest novel to be translated into English, practically begs you to read it as autobiography. Like The Changeling, as well as many other works not yet released in English, Death by Water is narrated in. . .
Jocelyne Saucier's Twenty-One Cardinals is about the type of unique, indestructible, and often tragic loyalty only found in families. For a brief but stunningly mesmerizing 169 pages, Twenty-One Cardinals invited me in to the haunting and intimate world of the. . .
We know so very little; so little that what we think to be knowledge is hardly worth reckoning with at all; instead we ought to settle for being pleasantly surprised if, on the edge of things, against all expectations, our. . .
Many of Virginie Despentes's books revolve around the same central idea: "To be born a woman [is] the worst fate in practically every society." But this message is nearly always packaged in easy-to-read books that fill you with the pleasure. . .
Ilja Leonard Pfeijffer's La Superba is appropriately titled after the Italian city of Genoa, where, after escaping the pressures of fame in his own country, the semi-autobiographical narrator finds himself cataloguing the experiences of its mesmerizing inhabitants with the intention. . .
It took reading 44 pages of Intervenir/Intervene before I began to get a sense of what Dolores Dorantes and Rodrigo Flores Sánchez were up to. Recurring throughout these 44 pages—throughout the entire book—are shovels, shovel smacks to the face, lobelias—aha!. . .
As presaged by its title, contradiction is the theme of Peter Stamm's novel, All Days Are Night. Gillian, a well-known television personality, remains unknowable to herself. And Hubert, a frustrated artist and Gillian's lover, creates art through the process of. . .


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Up Close and Personal
Out with the dinosaurs, in with tornadoes: It's time for the periodic changing of the guard at the IMAX 3-D theater at the Denver Museum of Nature & Science. Replacing DinoMax, Born to Be Wild and Under the Sea are the 3-D, large-screen mindbenders Tornado Alley and Flying Monsters (okay, that's another dinosaur movie), which will both play several times a day at the theater in an open-ended run.
Tornado Alley, which follows Sean Casey of Storm Chasers and his fleet of customized vehicles equipped with high-tech gear and a 70mm camera, promises to be an edge-of-the-seat thriller. And David Attenbourough's Flying Monsters is certain to scare the living daylights out of you as it explores the rise and fall of the pterosaur, a little-known prehistoric flying beast with a forty-foot wing span. You know what IMAX does to even the most minuscule things; now imagine that with an airliner-sized winged reptile, in 3-D. Whoa.
The IMAX 3-D Theater, located on the second floor of the museum, at 2001 Colorado Boulevard, is open daily; for tickets, $6 to $10 (museum packages are also available), go to www.dmns.org/imax or call 303-322-7009.
Oct. 8-July 12, 2010
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Denver, CO 80205
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Welcome! You are browsing as a guestWould you like to see 24 projects made from this yarn and much more? join Ravelry now What am I missing?
yarns Hamanaka ハマナカ Karoyaka Cotton (かろやかコットン)
by ribbonxxx Flickr
© hiro25neko
70% Cotton, 30% Polyester
Gauge:20-21 sts and 27-28 rows (10cm)
in Japanese:かろやかコットン


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Marrow Drive a Success
Another bone marrow registry drive was held at St. Bede's church on Sunday, Jan. 16 on behalf of Michael, a local high school student with leukemia. Among those participating were St. Bede parishioners, firefighters (including one from Station 82), PCY school parents, friends of friends, and many members from the La Cañada Presbyterian Church.
The fourth [...]
January 27, 2011 | Posted in Youth | Read More »


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Scarlett, you really can't judge a seedling based on maiden bloom. Let them bloom at least one more year before you decide. They usually get worse but they can get better.
Betty W.
-----Original Message-----
From: Cordesview <firstname.lastname@example.com>
To: iris-photos <email@example.com>
Sent: Tue, May 24, 2011 10:51 am
Subject: Re: [iris-photos] Ruffly style arms.
Hi Scarlett,
Oh, such a good turnout for your first seedlings!! I really like the Picture Book X Parisian Dawn seedling, too. Nice colors.
Regarding keeping the ruffly stigmatic lip -- it's literally up to you. Do you have room for it? Do you have patience to see it bloom again, maybe the color will improve a bit. Or, if it has great plant qualities and nice buds & branching, maybe you could keep it and use it as a parent. Even though you don't like the colors, it has all the qualities of the parents you liked and used to create it. :-)
Happy for you!
Adam Cordes
President - Mio Irisarians
Region 6
Zone 4 (and a half . . .J)
Like us on Facebook! ~ 'Mio Irisarians' ~
On May 24, 2011, at 7:08 AM, firstname.lastname@example.com wrote:
One of my crosses had the most amazing ruffly style arms. Didn't like the col or. Should I keep it?
Picture Book X Coral Point
There is a stigmatic lip in there somewhere.


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Lincoln and Reconstruction
Last week I was honored to be part of the Lincoln Legacy Lectures at the University of Illinois Springfield. Allen Guelzo and I spoke on aspects of Lincoln and Reconstruction … well, except for the fact that Allen was under the weather, and so our host, Michael Burlingame, read Allen's paper. Anyway, here it is.
Mike Pence at Gettysburg
I found this video of Indiana governor Mike Pence's visit to Gettysburg earlier this month to be an interesting overview of what Indiana regiments did at the battle … and John Hoptak did the NPS proud, as could be expected.
On Arizona Politics …
There is nothing quite like being a call-in guest on Phoenix morning radio while looking at Abraham Lincoln's home in Springfield.
It's an interesting life.
On Museums and History: The Case of Clarence Thomas
Whenever a museum recording the history and culture of its subject opens, we are eager to see the stories they tell, in large part because musums are more than the mere display of artifacts. Museums, like books, tell stories, and all narratives are inherently interpretive and represent choices with consequences.
Not everyone will agree with the result, as we saw at Gettysburg when the new visitors center and museum opened several years ago. I understood and sometimes agreed with the critics of the museum, although I think some of those criticisms were overstated. At the same time, I knew that the museum was tasked to do something more than to recount the events of July 1-3, 1863, and November 19, 1863, in isolation, bereft of any context. Yet I also knew that the context would represent interpretive choices that would be open to analysis and criticism.
So it comes as no surprise that some people are not too keen on everything they've seen in the Smithsonian Institution's new museum, the National Museum of African American History and Culture. Of especial note is the claim made in some corners that the museum more than slights the career of current Supreme Court Justice Clarence Thomas, supposedly, one infers, because of Thomas's conservative political views.
You know what's going to follow this report: whining about political correctness and the supposed political agenda of supposedly left-wing academics made by right-wing scholars, buffs, and "observers" (advocates) who claim that their adherance to their own political points of view is an expression of their dispassionate objectivity about the American past. We've seen that exercise in projection all too often.
Having not yet visited the museum (I haven't been to DC since it opened, but it is first on my list for my next visit), I can't testify as to the particulars of the case. Nor do I know how the museum treats other divisions among African-Americans over time, whether it be Frederick Douglass versus Martin Delaney, Booker T. Washington versus W. E. B. DuBois, or Martin Luther King, Jr., versus Malcolm X, all of which have been traditional (and overused as well as sometimes distorted or misunderstood) ways to view aspects of African American history. And that's just for starters.
That said, one hopes that in recognizing the diversity of the American past through focusing on the African American past, the museum also embraces telling the story of the diversity within the African American past … and present. Part and parcel of that discussion is an exploration of black conservatism as demonstrating that African Americans are far from a unified bloc when it comes to many issues. At the same time, one should not make more of something than is appropriate … we've seen that in the discussion of whether Africans Americans served in significant numbers as soldiers in the ranks of the Confederate army. I note that when it comes to that issue, those gallant defenders of historical accuracy often shed their disguises to suggest that, regardless of what Confederates themselves said at the time, such people were soldiers and should be recognized as such … a position that reflects their own presentist attitudes and political agendas. Others just let that issue go unexplored lest they offend their fellow travellers.
Not that we're about to hear those storied critics of political correctness hold themselves to the same standards they demand of others. No way. Otherwise we might have heard a reaction to this.
So let's actually visit the museum before we pass judgment. Otherwise we're little more than a clipping service bound to our personal prejudices.
The Confederate Flag … The Conquered Banner
We've heard a great deal about the importance of the Confederate flag (in all of its variations and manifestations, but primarily the Confederate Battle Flag). We are told that Confederate heritage advocates are honoring their ancestors by battling to keep the flag flying, implying that such is exactly what those folks would have wanted to do.
Not so fast.
The Rev. Abram Joseph Ryan was a Catloic priest based in Knoxville, Tennessee. He was also a poet as well as passionate Confederate. Some people called him "Poet-Priest of the South," while others dared proclaim him the "Poet Laureate of the Confederacy." In any case no one could question his fidelity to the cause.
Father Ryan had a most interesting Civil War career. Despising Abraham Lincoln, he shorted his first name to Abram; he protested being stationed in the North by his order, and by 1863 we have rports of his being with the Army of Tennessee, without other accounts placing him at Franklin and Nashville near the end of 1864. In the immediate aftermath of Confederate surrender, he composed the following poem, which first appeared in of all places, a New York journal:
The Conquered Banner
Furl that Banner, for 't is weary;
Round its staff 't is drooping dreary:
Furl it, fold it,—it is best;
For there 's not a man to wave it,
And there 's not a sword to save it,
And there 's not one left to lave it
In the blood which heroes gave it,
And its foes now scorn and brave it:
Furl it, hide it,—let it rest!
Take that Banner down! 't is tattered;
Broken is its staff and shattered;
And the valiant hosts are scattered,
Over whom it floated high.
Oh, 't is hard for us to fold it,
Hard to think there 's none to hold it,
Hard that those who once unrolled it
Now must furl it with a sigh!
Furl that Banner—furl it sadly!
Once ten thousands hailed it gladly,
And ten thousands wildly, madly,
Swore it should forever wave;
Swore that foeman's sword should never
Hearts like theirs entwined dissever,
Till that flag should float forever
O'er their freedom or their grave!
Furl it! for the hands that grasped it,
And the hearts that fondly clasped it,
Cold and dead are lying low;
And that Banner—it is trailing,
While around it sounds the wailing
Of its people in their woe.
For, though conquered, they adore it,—
Love the cold, dead hands that bore it,
Weep for those who fell before it,
Pardon those who trailed and tore it;
And oh, wildly they deplore it,
Now to furl and fold it so!
Furl that Banner! True, 't is gory,
Yet 't is wreathed around with glory,
And 't will live in song and story
Though its folds are in the dust!
For its fame on brightest pages,
Penned by poets and by sages,
Shall go sounding down the ages—
Furl its folds though now we must.
Furl that Banner, softly, slowly!
Treat it gently—it is holy,
For it droops above the dead.
Touch it not—unfold it never;
Let it droop there, furled forever,
For its people's hopes are fled!
Just sayin'.
Second-Guessing Bobby Lee
Several years ago I offered my observations on the challenge that Virginia posed to Ulysses S. Grant in 1864. But I've also pondered what choices Robert E. Lee had in the eastern theater. After all, people second-guess Lee's offensive strategy all the time, and yet I wonder what else he was supposed to do. Abandon Virginia? Stand on the defensive and absorb punches? Leave the defense of Virginia to someone else and go elsewhere to try to win the war?
Say you concede that none of these alternatives offer any more hope of Rebel victory. Did Lee's strategy, which featured counterattack followed by offensive operations across the Potomac, offer a real chance of victory? Or did his efforts in Virginia in June, July, and August 1862 drain his army of the manpower it needed to conduct successful offensive operations north of the Potomac that September? Did the bloodletting at Gettysburg compromise his ability to deal with Union forces in Virginia in the fall of 1863 and the spring of 1864? What were his alternatives, and did they offer more promising results?
These are not idle questions. Many historians argue that Lee's performance in the East was the key to Confederate persistence and gave the Confederacy its best chance to win. Fair enough. But that didn't happen, and, absent an unlikely and complete disaster on the battlefield, it was unlikely that the Confederates could have prevailed in that way. That leaves wearing out the Union's will to persist. Was Lee's approach likely to achieve that goal? Remember, the war was more than Virginia: I argue that Grant's ability to neutralize Lee in Virginia in 1864, despite Lee's best efforts, allowed the Yankees to prevail elsewhere in time to secure Abraham Lincoln's reelection. It's notable that after Sherman took Atlanta that September, the Confederacy failed to devise a game-changing approach to military operations in the two months that remained until election day.
So you tell me what you think … including you, Mark Snell. This one's for you. 🙂
Connie Chastain's War on Women … And Guess Who Supports Her?
It's not been a good week for Confederate heritage advocates. They are unhappy about what's happened at the University of Mississippi, where it appears that advocates of flying a Confederate flag have had a rough time telling the truth as they suffer setback after setback. Now comes news that the favorite candidate of most Confederate heritage advocates, Republican nominee Donald J. Trump, is in serious trouble because of his crass and lewd remarks (and that's kind) anout women, which most observers believe includes an endorsement of sexual assault. No word yet from Virginia Whine Country, which has been very protective of candidate Trump, about whether protests against sexual assault are simply new examples of political correctness, the target of many a mindless post that are little more than a clipping service of the alt-right. We await efforts to find a Gordon Wood quote that can be twisted in suppport of that blogger's position.
Without a doubt, however, one need only look at what Virginia Flaggers spokesperson and webmaster Connie Chastain says about Trump to understand that some corners of Confederate heritage advocacy are also busily engaged in conducting a war on women. Like Trump, Chastain favors criminalizing the decision of women to have abortions:
Even Trump has thought better of this, but Chastain has not.
And, just like our friend at Virginia Whine Country, Chastain decides it's all the fault of the left and the media:
Note the identity of the single retweeter. Note also that I guess that one can conclude that Trump's past is irrelevant, but not so Clinton's past … or that non-leftists don't care about treating women with respect.
Of course, one remembers that Chastain was also a big fan of claiming that women often made false claims about being raped (unless the person being accused was Bill Clinton, I guess). This was a theme of two of her underwhelmingly successful novels. Endorsing sexual assault as a way to approach women is okay in Chastain's mind, because we can set it aside as "locker room talk"; that such talk, if put into action, results in rape seems irrelevant (will it elicit more squawking from her about false rape accusations?).
It's a small step from here to the reasoning of sexual predators that women "really want it" and that "no" means "try harder."
After all, just saying one's sorry is good enough.
Note who retweeted both of these as well.
After all, Chastain wants Trump to whack people, including Clinton:
Funny that she chose that means of expression.
Mind you, Chastain thinks something's wrong with women ("cackling hens"?) who protest such treatment:
Moreover, "real women" wouldn't get upset:
That's right … this controversy about advocating sexual assault is simply "nothing," and the best proof comes from the sales of a book that is not Chastain's (jealous, Chastain?). Let's mock the outrage over such behavior as nothing more than make-pretend about "women's delicate widdle feelings."
What else could you expect from someone who seems obsessed about false rape accusations? So … anger over language advocating sexual assault is nothing more than an act to placate "women's delicate widdle feelings"? Really?
(Yes … Chastain's gone the fake-name route in crafting an alternative identity of "Polly Graff," although it did not take her long to drop the pretense that it was her.)
We await Chastain's efforts to identify which men engage in such talk. As her previous interaction has been with Republican politicians, Confederate heritage apologists, and false rape accusation protesters, we can't wait to hear about her experiences about what is said in these locker rooms.
But don't mistake Chastain for a feminist …
Note the wording: it's not "pseudo-feministic women," but "feministic pseudo-women." Real women aren't feminists: feminists are "pseudo-women." And no, folks, that's not a mistake … she says "pseudo-women" twice.
We await the usual retort from Virginia Whine Country that protesting lewd talk and endorsements of sexual assault are nothing more than exercises in political correctness from a leftist academic who's attacking a political candidate … because, apparently, these things should not be attacked. They are nothing more than locker room talk, as if that's an acceptable excuse.
Finally, note who likes (and retweets) Chastain's Twitter activity on behalf of sexual predators … Susan Hathaway. The fact is that Hathaway and her followers have politicized their movement in explicit ways by endoring Trump, not just as individuals, but as an organization:
Oh, well.
The heritage of hate continues. If anything, it's grown, because it now includes people who protest sexual assault and the victimization of women not named Susan Hathaway.
Oh, My … Flagging for The Donald?
Most fair-minded people would agree that Hillary Clinton was wrong when she claimed that half of Donald J. Trump's supporters were "a basket of deplorables." That claim painted with too broad a brush that tarred too many people.
That said, it is true that some of Trump's supporters are truly deplorable, especially those who have been associated with what one Pensacola blogger claimed was the future of Confederate heritage … the Virginia Flaggers.
Susan Hathaway supports Trump. Connie Chastain supports Trump. Matthew Heimbach, once a Flagger darling, has actually attacked protesters at a Trump rally.
Chastain's political views, indeed, appear to be straight out of the Trump playbook.
We've seen Confederate flags at Trump rallies, which does little to promote the notion that either the Trump campaign or Confederate heritage is especially tolerant. For Trump supporters outside "the basket," it's an unfortunate association.
And then came Friday's revelation of a video, some eleven years old, of Trump and television program host Billy Bush making crude and lewd comments about women.
Guess who came out swinging for Trump? That's right, Connie Chastain. Given her obsession with the notion of false rape accusations … a theme that has shaped several of her works of fiction … one might well expect that she would shrug off Trump's crass talk that smacked of sexual assault and rape. And so she did (check the comments section … where Chastain posts under her own name as well as the name "Polly Graff").
So much for southern womanhood. We recall that Susan Hathaway once said that Chastain did her "heavy hitting." Now we have a better idea of what that means.
World War I Comes to Your Video Game Console
Once upon a time World War II was a favorite subject of video games, especially first person shooters (FPS). It's where the series "Call of Duty" got its start, first on personal computers, then on video game consoles. Nowadays such series foucus on modern and future combat, weaving missions into narratives that question the very subject of the game (and the interest of the player).
Previous conflicts have not fared so well in the FPS genre. Efforts to present the Civil War on video game consoles proved feeble. Here and there PC efforts gained an audience, but on the whole the pre-World War II period were represented by titles that explored conflicts, campaigns, and battles. One exception was aerial combat, but that seemed to be in part because the differences between World War I and World War II dogfighting were not great (as opposed to the heat-seeking missiles, sometimes offered in incredible payloads, for more modern games such as the Ace Combat series).
This fall, however, video game consoles will turn to World War I with the appearance of Verdun (a PC port) and Battlefield I. The makers of the latter game have offered twelve minutes of in-game action for us to absorb.
Of especial note is that when "you" die, you die. As a player, you assume someone else's character, but the character that dies is not doing to be revived by the reset button or some other eternal life device.
Having not played Verdun and with Battlefield I delayed for the XBox1, I can't tell you how the games play, or how they simulate anything. We'll see whether a grittier view of war is something that video gamers want to explore.


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Stephen Seckold
Executive Chef
When Stephen Seckold opted to do high-school work experience in a busy restaurant on the mid-north coast of NSW, he was instantly hooked.
"I always enjoyed cooking with my Mum from a very early age, and when I got into a professional kitchen for the first time I fell in love with the pace and creativity," Stephen recalls. "On my 15th birthday I started my apprenticeship at Bluewater Brasserie, close to home in Nambucca Heads."
After moving over to the kitchen at Bonville Golf Resort, south of Coffs Harbour, Stephen went on to complete his training at Sydney dining institution Jonah's, in Whale Beach.
"The restaurant is constantly evolving, but it's the spectacular setting, consistent high quality and passionate staff that will always keep everyone coming back."
"Jonah's was the first hatted kitchen I worked in and it really elevated my skills," says Stephen. "After my apprenticeship I stayed on as a Commis Chef under Richard Millar, who taught me a lot about the importance of working closely with suppliers and never compromising on produce quality."
Next came a posting with Novotel Pacific Bay Resort, back in Coffs Harbour, where Stephen worked as Senior Chef de Partie before being lured back to Sydney to join Peter Kuruvita at Flying Fish Restaurant & Bar.
"I worked alongside Peter as Head Pasty Chef and Sous Chef for four years, and was responsible for helping to oversee and run all kitchen departments," he explains. "It was really inspiring to be showcasing Australia's finest seafood while getting creative with a mix of Asian flavours."
Keen to gain overseas experience, Stephen headed to London and took on the role of Chef De Partie under Bjorn van der Horst at La Noisette, a Michelin-starred restaurant opened by Gordon Ramsay in the mid 1990s. This proved to be a defining experience.
"La Noisette didn't follow any traditional cooking guidelines – Bjorn always cooked freestyle, so no two guests ever received the same dishes," says Stephen. "Working in this way really taught me that cooking is a never-ending learning process, and to always be open to new ideas."
Ready to embrace another challenge, Stephen signed up for a stint in Botswana, consulting for a travel company and writing menus for high-end lodges. "I had an amazing time, and developed an obsession with recreating a Portuguese-style chicken dish I tried in Mozambique and using chilli and lemon in my cooking," he laughs.
Rejoining the Flying Fish Team
In 2008, Stephen was invited to head up the kitchen at Flying Fish Fiji, located on the luxury grounds of Sheraton Denarau Villas. It was a welcome opportunity to consult on Peter Kuruvita's innovative menus and train a team of 18 Fijians to produce high-quality cuisine.
"Island living was really exciting, and it was amazing to watch our team develop their skills and really embrace the opportunity to learn and work together," he says. "It was an invaluable learning experience for me as well."
After spending two years honing his management skills and establishing Flying Fish Fiji as a destination in its own right, Stephen returned to Flying Fish Sydney as Head Chef, where he continues to get creative with the finest seafood and seasonal ingredie nts.
"Freshness, seasonality and working with an innovative team are the things that always keep me inspired in the Flying Fish kitchen," he adds. "By keeping up with the seasons we get a new canvas and a new set of colours to work with every three months – in summer we go light, crisp and tangy, and in winter we go rich, warm and hearty."


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A four-year-old girl from Nottingham has died after being hit by a car on a Florida beach.
Ellie Bland was walking alongside her great-uncle on Daytona beach when she was struck by a car on Saturday. The vehicle is believed to have been travelling under the 10mph speed limit at the point of impact.
Onlookers had shouted for the driver to stop as the girl lay in front of the vehicle. But officials claim that after coming to a halt, the driver appeared to panic and advance forward, running the girl over and killing her instantly. "For an unknown reason, the driver panicked and hit the accelerator instead of the brake," police said.
The girl's great-uncle, John Langlands, 53, told The Daytona Beach News Journal that the car, a silver Lincoln, had come "barrelling down" on the pair, giving him no time to pull Ellie out of the way.
The driver has been named as Barbara Worley, 66, from Elberton, Georgia. An investigation is underway, though police said no charges have yet been filed.
The death is likely to intensify calls for a ban on the practice of driving on Daytona beach, a tradition dating back to the early days of the automobile. The highway patrol said it was not unheard of for people to be injured by cars on the beach, though the Journal claimed it was Florida's first beach traffic death for 14 years.
Ellie was on her sixth trip to Florida, accompanied by her five-year-old sister, her great-aunt, great-uncle and family friends.
Yesterday a Foreign Office spokeswoman said: "We can confirm the death of a British national in Florida on 20 March. The next of kin have been informed and we are providing them with assistance."
- More about:
- Beaches
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And as much as effusive "Modern Family" star and guest judge Jesse Tyler Ferguson would like all the dancers to carry on for another week, two more members of the "So You Think You Can Dance" Season 8 cast will fall during Thursday's results.
So before we tear these happy partnerships apart, forcing the remaining 10 into the strong, experienced arms of the "SYTYCD" all-stars, they get one more night together.
And two dances.
But who made the best of their first double feature — and who's almost definitely dancing for their life this week? We've got analysis, couple by couple, right here:
Alexander and Sasha
Dance #1: Tony Meredith and Melanie Lapatin (she of the greatest "SYTYCD" moment ever) choreograph a postmodern Paso Doble where the dancers play dueling matadors. The judges don't go overboard in their praise, but Sasha gets higher marks than Alexander.
Dance #2: The tables turn, and the judges say that Alexander bests Sasha. Nigel even goes so far as to say that she's been carrying him. Not anymore. Still, neither of their dances really broke through — and they could easily be in the bottom tomorrow.
Tadd and Jordan
Dance #1: Travis Wall scores again with a super-dark contemporary routine about vulture trying to eat some dying dude who ends up snapping its neck. (No, really.) Jordan's leg lifts are particularly amazeballs, and the judges go wild.
Dance #2: Weird from beginning to end, this Broadway tune with bonkers outfits and a "Rent" soundtrack left the judges underwhelmed. Their first routine should be strong enough to keep them safe from elimination though.
Ryan and Ricky
Dance #1: If you're going to dress a duo up like Audrey Hepburn and Fred Astaire in some bizarre old movie fan-fic, you're going to have to dance better than this. The Broadway routine left us feeling cold and increasingly unhappy with Ryan.
Dance #2: Could this be a "SYTYCD" first? Mary Murphy awards the rigorous Cha Cha with a ticket on the hot tamale train for… just one of the dancers. Ryan was left at the station, while Ricky rode off to whatever town the HTT visits.
Catlynn and Mitchell
Dance #1: You could tell the judges were going out of their way not to slam choreographer Christopher Scott for his melodramatic piece about Ugandan children forced to serve in the military, choreographed to some disco remix of Lupe Fiasco. At it's best, it's awkward. At it's worst, it's offensive. Sorry guys.
Dance #2: Travis Wall does it again, blessing the duo with a redemptive jazzy number that washed the sour aftertaste from that Uganda mess from our dance palates. Still, they're in jeopardy.
Melanie and Marko
Dance #1: Oh hey, Louis Van Amstel. The "Dancing With the Stars" pro choreographed a tango for this season's strongest couple, and they delivered on every level. Nigel even thought that it was their biggest challenge of the season.
Dance #2: These two can do no wrong in our book, and with a contemporary routine Dee Caspary, they have two incredibly strong dances going with them into tomorrow's votes.
Jess and Clarice
Dance #1: This routine may have won us over… if it wasn't for the props. The whole concept (a girl who doesn't like the way she looks, set to Bruno Mars' "Amazing") felt too literal, and Jess has a hard time selling hip-hop. We don't know how the audience didn't burst into laughter when he turned around the set-piece to reveal a portrait of Clarice.
Dance #2: Hot damn, a dance without a backstory! This episode-ending jive was just straightforward fun, though the judges did think the duo lost steam towards the end. And who could blame them? This show's been going on for two hours.
So who's it going to be, folks? We're ready to say goodbye to Ryan, but we're still torn over the boys.


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October 20-22, 2017 Buffalo Thunder Resort & Casino
Santa Fe Comic Con is held at the beautiful Buffalo Thunder Resort & Casino.
Featuring Actors, Entertainers, Comic Book Artists and many more! POLICE , MILITARYand FIRE FIGHTERS get in for FREE! Just bring your commission card, badge, or current active duty military ID to get free admission into the event.
Get more information HERE or click below to make your reservation.
Buy Tickets Now!
Tickets are available online!
2016 Guests
Actors, Entertainers, Comic Book Artists and many more!
Show Hours
Friday 4pm-8pm
Saturday 10am-8pm
Sunday 10am-6pm
get information on becoming a vender HERE


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GTX 71 440 vs. RT Charger 68 360 six pak
On a 201 meter legal street race in Denmark. No burnout, puplic street, there is BF on the RT and Nitto soft comp. on the GTX.
1968 Charger VS. Lamborghini's in the autocross.
Well... I had a chance to run MR ANGRY against some REALLY stiff competition today... a bunch of Lamborghini Gallardo's... WOOF!
Charger Vs Viper
Mora-Johan in action =)
STREET RACING COMPILATION LONG 2014 - 1 Hour Traffic Street Racing Compilation - NO.1
Subscribe ► http://bit.ly/1NUdxDs 1 Hour Street Racing Compilation 2014-2015 (Best Of) Full Video with original sounds. Subscribe and Drive Safe! Disclaimer: I do not condone street racing in any way


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Banks beef up quant solutions for FX in response to buy-side demand
As electronic trading takes hold in foreign exchange, and regulatory changes in the US and Europe to improve reporting and trading transparency kick in, the buy side is demanding more information regarding how trades have been, or should be, executed. And some banks are beefing up their foreign exchange quantitative offerings in response. Quantitative services vary from bank to bank, but over the...
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The Cathedral Prep Ramblers basketball team won Wednesday's neutral playoff game against Central Catholic in overtime by a score of 50-49.
Game Details: PIAA AAAA Second Round Playoff @ Sharon H.S.
This game is a part of the "2012 Pennsylvania Boys State Basketball Playoff Brackets: PIAA - AAAA" tournament.
In Wednesday's non-league match, the visiting Cathedral Prep Ramblers basketball team scored a tight 50-49 triumph over the Central Catholic Vikings.
In their next game, the Ramblers confront the Shaler Area Titans (Pittsburgh, PA) in a non-league match, on Saturday, March 17. Cathedral Prep will attempt to move on its 20-5 season record. The Titans enter the match with a 25-3 record after their 62-49 non-league win over Gateway (Monroeville, PA).
With the defeat, Central Catholic moves to 16-12 on the year.


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The much-trumpeted reforms to international banking regulations are not enough to ensure financial stability, the chairman of the Financial Services Authority (FSA) said last night.
The head of the soon-to-be-dismantled financial watchdog said that in an ideal world Basel III would have required banks to have core capital of 15 - 20 per cent, rather than the 7 per cent it specifies, but regulators have had to keep clear of rules that might slow down the economic recovery.
In the same vein, Lord Turner added that while it was desirable to deal with banks that were too big to fail by making sure that there were ways to impose losses on debt holders, current plans were not "sufficient to address risks of systemic instability".
Moreover, he said rulemakers needed to take a closer look at the risks attached to the so-called "shadow banking" system populated by hedge funds. Fixating on traditional banks alone would leave the door open for another crisis, he said in a speech at City University's Cass Business School.
"The pre-crisis delusion was that the financial crisis, subject to the then-defined rules, had an inherent tendency towards efficient and stable risk dispersion," he said. "The temptation post-crisis is to imagine that if we can only discover and correct the crucial imperfections – the bad incentives and structures – a permanent, more stable financial system can then be achieved.
"It cannot, because financial instability is driven by human myopia and imperfect rationality as well as by poor incentives, and because any financial system will mutate to create new risks in the face of any finite and permanent set of rules," he added.
The FSA chairman's comments come less than a week after Axel Weber, the European Central Bank member and the head of Germany's Bundesbank, highlighted the need to deal with the wider financial system, not just banks. "We need to shine some light into the shadow banking system," Mr Weber told students at University College London.
"Basel III and special rules for systemically important institutions are certainly important instruments in safeguarding the stability of the financial system," Mr Weber said. "However, the best dyke is of little use when there is a whole ocean at our back."
Speaking last night, Lord Turner added his voice for greater attention on the farther reaches of the financial sector. "Since the crisis, global regulators have focused primarily on capital and liquidity requirements for banks, both in general... and for big banks in particular," Lord Turner said. "But the initial year of the crisis, 2007 to 2008, it did not seem like a familiar banking crisis, but something entirely new, a crisis of 'shadow banking'."
When it comes to the traditional banks that are too big to fail, Lord Turner said it was important to ensure that were mechanisms were in place to ensure that authorities can impose losses on all debt holders so that problematic institutions may be recapitalised without resorting to taxpayer support. Equally, it was important to ensure that the banks themselves can absorb losses.
But such measures alone cannot provide protection against the sort of systemic meltdown seen during the dark days of the recent crisis. Other steps, such as equity surcharges on the biggest banks, should be pursued, he argued. The possible break-up of the biggest banks – the pros and cons of which are being looked into by the Independent Commission on Banking – should also be considered, Lord Turner said. "For the UK, with major banks which are very large relative to our GDP, a key policy objective for this year is to ensure that Financial Stability Board decisions on systemically important financial institutions result in higher-than-Basel III equity requirements for most systemically important banks."
He added it was important to keep a closer tab on the industry to ensure rules keep pace with systemic shifts.
- More about:
- Banking
- Basel
- Cass Business School
- European Central Bank


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Mel-8704R Meter - EMF, Temperature, & Flashlight in One Handheld instrument
You save
This is the "Basic" Mel Meter that was introduced in 2008. This instrument includes all of the essentials features used by Professional Paranormal investigators. However, it does not include an expansion slot. The expansion slot is a cost saving feature that allows the device to be upgraded by DAS with new features at any point in time. If you think that you may out grow the basic Mel Meter, select the Mel-8704R-ProNav Model that has the expansion slot built into it.
Mel-8704R Basic Model
- Measures EMF, & ambient Temperature
- EMF "Burst" (100msec) mode feature for rapid EMF fluctuation
- EMF Min/Max capture
- Rapid response ambient temperature measurement
- Temperature min/max capture
- Red night view Backlight Display with On/Off Button
- High intensity Red Flashlight
- Includes: (1) 9Vdc Alkaline battery
- Includes: (1) Deluxe carrying case


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In New York City, you're forced to shut out a lot of things. Life pushes you forward, and answers are easy on an Ivy League campus.
I said goodbye to my parents and little brother. I was off to live in San Francisco, where I could watch the skies change, where the light stuck to the hills.
And this holiday, I resolved to do two things; teach my parents how to Skype, because I needed their furrowed brows and their smiles and to volunteer my time. Goodness knows, I had plenty of it.
On Thanksgiving, I delivered meals to the poor. My friend marveled that at 8:30 AM we could hand people their last hot meal of the day.
At a Tenderloin hotel, we knocked on doors, greeted with curses. Empty rooms reeked of vomit, urine, pot and that sticky odor of greed borne of desperation. It hung like curtains. I breathed into my neck scarf. Those who answered the door had big eyes, legs like toothpicks. They asked for more; we had to say no. We had a list to follow after all. If people didn't answer, we couldn't leave the meals. The receptionist said they'd be stolen. So we ran back collecting them from doors never open to us.
Residents crowded around our empty boxes, not believing we had run out, accusing us of being unfair. We were. We believed our little mission somehow helped while we drove around in a BMW distributing the rest of our meals through the window. Our knowledge and good intentions weren't even a blip on the radar. We were scared that people could live that way and think it was normal. Scared that our world was not made of ivory and stone — but scribbled with crayon and easily torched.
Afterward we went to brunch in Cole Valley and washed our hands in a bathroom not stained with human excrement.
My family finally came through on Skype via my iPhone – all smiles and gentle chiding about California living. They teased me about my long hair and my "hippie" jacket. They're novices, blissfully ignorant. It was all I needed to feel like the most privileged person on Earth.
With a Perspective, I'm Jen Haley.


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Cash Back:
This item is in stock in our East and West Coast warehouses
The D-D Midday 6000 T5 bulbs have full a 5 point colour spectrum and 6000K colour temperature which exactly recreates natural sunlight.
Suitable for salt water and fresh water. Midday is a 6000 Kelvin tube with a 5 point spectrum which recreates sunlight. It is suitable for salt water, fresh water, and reef planted or fish only aquariums.
This lamp is ideally suited to full spectrum use for growth of SPS and also works great when used in a 1:1 ratio with either the Actinic, or Blue supplementary lamps.
Available Colors:
Actinic+:A special 60/40 combination of deep blue and actinic wavelengths emits levels of actinic as well as a blue spectrum. Suitable for saltwater and reef aquariums. Color Temperature: Blue
AquaBlue+:A 60/40 white/blue blend. Specifically developed for growing SPS corals. Suitable for saltwater, reef and freshwater aquariums. Color Temperature: 11,000k
AquaFlora:The perfect light spectrum for lush plant growth. Specially designed for freshwater use. Highlights the red and blue colors of your fish. Suitable for freshwater aquariums.
AquaPink:Specially designed for marine use. It brings out the blue, violet and red colors in your SPS and fish whilst enhancing coral growth. Suitable for saltwater and reef aquariums.
Midday:The D-D Midday 6000 T5 bulbs have full a 5 point spectrum and 6000K color temperature which recreates natural sunlight. Suitable for salt water, fresh water, reef and fish only aquariums. Color Temperature: 6000K
Pure Actinic:A complete pure actinic spectrum for maximum fluorescence from your corals. Suitable for saltwater and reef aquariums. Color Temperature: Actinic.
Bulb Replacement Guide Bulb Types
Life of Bulb*
Normal Output Fluorescent
6-12 months
Very High Output (VHO)
6-12 months
Power Compact (PC)
9-12 months
T5 High Output (T5HO)
9-18 months
Metal Halide
9-12 months
LED (non-moonlight)
~5 years (50,000 hours)
* Manufacturer Recommendations
D-D The Aquarium Solution
D-D The Aquarium Solution supplies freshwater and saltwater aquarists with high-quality products from Deltec, Rowa and Giesemann. The company only develops and introduces products to the aquarium marketplace that are technically innovative and that they themselves have tested and know will work. The company`s Managing Director, David Saxby, is known throughout the world as being at the forefront of modern reefkeeping and his 3,700 gallon home aquarium system has appeared on television and in many books. David formed D-D to bring his own personal experience of which products worked for him to the mass market and to dispel the myths and witchcraft that abound within the hobby. The success of D-D`s partnerships with companies such as Deltec, Rowa and Giesemann plus the development of a wide range of their own products has seen the company grow well beyond that original idea to become one of the most successful companies of its type in the marketplace today.
Manufacturer Warrant: 30-day warranty
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Just how different is Windows from Mac – Making the switch?
Are you a Mac or PC?
That has been the question raging on for the better part of three decades, and it doesn't look like it'll be stopping anytime soon. Windows is known for its totalitarian design and insane install base, yet Apple was the cool MacBook maker with eye catching aluminium designs and quirky ads poking fun at Windows.
How the tables have turned.
Microsoft has become a cutting edge PC maker with a 28-inch touch desktop and respectable laptop lineup while Apple is forcing users to live the dongle life. All of this begs the questions, which one is better?
When it comes to hardware, arguably Microsoft has caught up, but Apple is still Apple. That's no slight to Microsoft, the Surface Book is a great design, but the MacBook has dominated this arena for the better part of a decade. The Surface Studio looks stunningly beautiful, but the iMac still holds its own in the battle.
When it comes to software, that's where things even out. They both offer differing features and it really comes down to your preference.
Jon, who is a dedicated Mac user at work—his work machine is a 5K iMac—decided to switch to Windows and try it out. He switched to the Surface Book and has been using it ever since.
Did he enjoy the experience? Did he hate it? Is there even a difference between the two anymore? Jon found answers to those questions and more.
Check out the video above to see how it was to switch from Mac to Windows for Jon.
Article source: http://www.technobuffalo.com/videos/windows-vs-mac-difference-switch/


text: Chixy Natural may be outside of your area. Please enter your delivery address to check availability Do you need any... Napkins & Utensils Continue to Checkout Chixy Natural Back to Menu Your order refresh Chixy Natural Your Shopping Cart is empty edit Delivery Address Please enter delivery address edit Delivery Time N/A* Add someone to this order Chicken Caesar Salad Fresh romaine lettuce, Chixy's Caesar dressing, Parmesan cheese, croutons, sliced boneless chicken breast. Add Avocado?You may select one of the choices Add Avocado ($2.00) x $12.00 Special Instructions: Label Item For: or Cancel


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Publisher's Description+
From Anarchy Enterprises: Uncover secret treasures within the lockers of this Hidden Object game! Compete against a diverse cast of characters with a new twist - all the objects are affected by gravity and reshuffle as you find them. Every level is a new experience each time, randomized for maximum replay value. Pull up your sleeves and get ready to dig into some fun! FEATURES 36 Levels! Hundreds of objects! Compete against a zany cast of characters! Humorous stories for the objects you find! Every level completely randomized! Tons of replay value! Affordable Price!
What's new in this version:
Added dozens of new levels! Added character bios! Added special "rumor" objects to each level! Added summary screens to each level with funny stories about the unique objects you find, and their $ value!
read more +


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The makers of The Jedi Path and Book of Sith have a new book coming out later this year: The Bounty Hunter Code: From the Files of Boba Fett, and Paul Nicholasi of Idle Hands got a sneak peek of it at BookExpo America this past weekend. Like the previous two books, it is set as an 'in-universe' document, comes in an ultracool electronic case, and has a handful of inserts. In-universe commentary is provided by such galactic denizens as Boba Fett, Bossk, Aurra Sing, Greedo, Jango Fett and Hondo Ohnaka!
As items collected by Boba Fett prior to his dip in the Sarlacc, The Bounty Hunter Code includes a copy of the Bounty Hunters Guild Handbook and a volume from Death Watch, and Cradossk's memoirs, and is scheduled for release in October 15, 2013. Amazon lists the authors as Dan Wallace (author of The Jedi Path and Book of Sith), Ryder Windham, and Jason Fry. Hop on over to the link above to see some pages and art from the book, including instructions on how to hotwire a landspeeder.
From the description on Amazon: Continue reading


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Logo design and print branding for a new neighborhood
- Status Closed
- Budget $2 - $8 USD / hour
- Total Bids 21
Project Description
Project Description:
I am a real estate agent that just landed a new neighborhood to list all homes for sale, Lower price point of homes. I need a logo and print pieces that is not too sophisticated or edgy. The logo and print pieces need to be eye catching and memorable, apart from that you have full creative freedom to come up with some samples. The logo and print pieces are required ASAP so would like to see initial drafts with a few days.
My expectations:
1) If you are interested in taking on this project then start by placing a bid.
2) show me a sample broadly based on my design brief, I need to see what you can do for me.
3) If I like your work and your bid is reasonable then i will award you the project and make 20% of the payment.
4) You then begin work on the project and get back to me with drafts, queries etc.
5) Once you have shown me a few designs that I like and I have selected the final design, I will make 40% of the payment.
6) Once you send all source files and hand over all rights to me, I will then make the final 40% of the payment.Get free quotes for a project like this
Skills Required
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- Set your budget and the timeframe
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Despite the important role of distant-acting transcriptional enhancers in human biology and disease, identifying their location in the genome and determining their in vivo regulatory activities remains a major challenge. There is compelling evidence from a large number of recent genome-wide association studies that variation in noncoding genomic intervals contributes on a substantial scale to a wide range of traits and disorders. However, the paucity of comprehensive enhancer catalogues has largely precluded systematic studies of the underlying regulatory and etiological mechanisms. Accordingly, the central goal of the parent grant and this renewal application is to identify and define the in vivo activities of a sizeable set of enhancers in the human genome to serve as a springboard for broad community access. In funded years 1 through 3 (2006-2009), we established the power of extreme comparative genomics to identify a large collection of putative enhancers and have unambiguously assigned specific in vivo enhancer function to hundreds of human conserved sequences. However, this comparative approach fails to predict a priori which conserved noncoding fragments are indeed enhancers and, if so, where they will be active in vivo, thus requiring massive- scale transgenesis to resolve. More recently, we demonstrated the power of chromatin immuno- precipitation targeting an enhancer-associated transcriptional coactivator (p300) coupled with massively parallel next generation sequencing (ChIP-Seq) to accurately identify enhancers active directly in mouse tissues. This scalable experimental approach has assigned putative enhancer function to thousands of noncoding regions, thereby dramatically expanding access to genome-wide sets of enhancers active in particular cell types or tissues. However, while p300 represents a remarkable epigenomic marker for enhancer identification in vivo, it is just one member of a larger class of transcriptional co-activators and only marks subsets of known enhancers. Based on these findings, the present proposal involves the extension of these ChIP-Seq studies to 11 prioritized transcriptional co-activators followed by validation of enhancer predictivity for each epigenomic mark through a series of high-throughput transgenic mouse assays. It is anticipated that public access to these data sets will significantly fill our void in gene regulatory annotation of the human genome and to decipher how variation in these sequences causes human disease.
The generation of the entire human genome sequence serves as a routine starting point for a huge investigator base and has aided in defining the majority of genes in our genome. However, our understanding of the sequences that regulate these genes is meager, despite their presumed alterations in human disease. Here, we propose to identify and test epigenomic marks of DNA for their ability to act as gene regulatory sequences in transgenic mice. The identification of positive signatures of enhancers in vivo is expected to significantly fill our void in gene regulatory annotation of the human genome and to decipher their mutation as a cause of human disease.
|He, Aibin; Gu, Fei; Hu, Yong et al. (2014) Dynamic GATA4 enhancers shape the chromatin landscape central to heart development and disease. Nat Commun 5:4907
|Cheng, Yong; Ma, Zhihai; Kim, Bong-Hyun et al. (2014) Principles of regulatory information conservation between mouse and human. Nature 515:371-5
|Osterwalder, Marco; Speziale, Dario; Shoukry, Malak et al. (2014) HAND2 targets define a network of transcriptional regulators that compartmentalize the early limb bud mesenchyme. Dev Cell 31:345-57
|Attanasio, Catia; Nord, Alex S; Zhu, Yiwen et al. (2014) Tissue-specific SMARCA4 binding at active and repressed regulatory elements during embryogenesis. Genome Res 24:920-9
|Pattabiraman, Kartik; Golonzhka, Olga; Lindtner, Susan et al. (2014) Transcriptional regulation of enhancers active in protodomains of the developing cerebral cortex. Neuron 82:989-1003
|Dickel, Diane E; Zhu, Yiwen; Nord, Alex S et al. (2014) Function-based identification of mammalian enhancers using site-specific integration. Nat Methods 11:566-71
|Wu, Han; Nord, Alex S; Akiyama, Jennifer A et al. (2014) Tissue-specific RNA expression marks distant-acting developmental enhancers. PLoS Genet 10:e1004610
|Eckler, Matthew J; Larkin, Kathryn A; McKenna, William L et al. (2014) Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex. Neural Dev 9:6
|Gordon, Christopher T; Attanasio, Catia; Bhatia, Shipra et al. (2014) Identification of novel craniofacial regulatory domains located far upstream of SOX9 and disrupted in Pierre Robin sequence. Hum Mutat 35:1011-20
|Visel, Axel; Taher, Leila; Girgis, Hani et al. (2013) A high-resolution enhancer atlas of the developing telencephalon. Cell 152:895-908
Showing the most recent 10 out of 37 publications


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Getting Involved
Latreia's Winter Service Day
The next Latreia Service Day is coming up on Saturday, March 4. Students will be serving alongside great organizations within a few of Seattle's neighborhoods. Our pancake breakfast will start at 8:30 am in the SUB Gazebo Room before we head out to our sites for the day. Lunch is also provided.
Don't miss this opportunity to serve with your SPU community.
Contact firstname.lastname@example.com for more information.
Latreia's Winter Service Day
Saturday, March 4, 2017
8:30 am - 2:30 pm
meet in SUB Gazebo Room
Founded in 2004, the John Perkins Center is a first of-its-kind partnership between the John and Vera Mae Perkins Foundation of Jackson, Mississippi, Seattle Pacific University, and Christian community leaders throughout the Pacific Northwest. Learn about the John Perkins Center