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!Help! cant find pvp movie
Failure replied to yager's topic in Vanilla Wow General DiscussionTook me only a few seconds to find it: http://www.warcraftmovies.com/movieview.php?id=206207
BUG!about The Balance of Light and Shadow
Failure replied to consexp's topic in Vanilla Wow General DiscussionKill her and don't be afraid - she won't be fighting back; apart from a group of 100hp skeletons attacking you at some point of course. You'll figure it out =)
Mystic Dawnstone
Failure replied to brice12's topic in JewelcraftingStill waiting :3
Guilds be aware: "Healyourass" and "Dalibor" stealing from guild bank.
Failure replied to Aidoo's topic in Burning Crusade General DiscussionDon't even get me started on these two ^^
Using multi right after aimed shot?
Failure replied to Aers's topic in HunterAimed>Auto>Multi.
Need a macro command help!
Failure replied to Hisоkа's topic in UI & MacrosIn order to use modifiers different from shift-alt-ctrl you would need to make registry changes and that's not from within WoW. Aka You can't use mouse buttons as a modifier unless you've got a desire to invest some time in it.
pls ban peligros [A] for leeching in bgs
Failure replied to lop's topic in Archangel Player vs PlayerI can vouch for this guy doing nothing in BGs. He technically isn't AFK as he is moving about, however his gear is as green as ever no matter how much honor he racks up. Reporting him AFK will never work as he's got some noble quest to be active dead weight. Getting this guy banned? I hardly doubt that. With a slight poke of sarcasm as to why I think like that would probably get me a ban instead.
In hindsight - yep
I did and I continue being so; I've given some hints as to why I returned for the weekend in the first place and have yet to meet a person here who'd actually be able to comprehend the reasons behind me playing even to an average extent.
Perfection includes me having to play WoW for this weekend. Which is another long story with an extremely surreal plot Dragnu the shit leveling hunter is on the move.
I see what you mean for obvious reasons. And thank you for your honest kind words; it was a bumpy road back then but well worth it and you did well, too. You managed to balance me quite good until I really got girly about many things. But since it's a long story we might as well give it a go on Skype since otherwise we would scare away new recruits Edit: JOIN LCT PEOPLE! It was and always will be a guild with a soul. Drop me a Skype line in PM (if you find your old post-it note with my Skype contact on it you can rest assured that it's not in use 99%+0.9% of the time).
I guess if I had the time and if there was a good enough server to return to I would. And without Sil WoW isn't the same anymore and I know that for a fact that he's not planning to return; even to CC. Other than that I kind of miss dicking about.
Hihi. Extremely long story of an extremely long year short - pretty much perfect. Had to drop by the forums though (have been here before lurking around) because my spies with their little eyes spied something like Liebe reforming LCT so I just had to log my lovely account of many posts. And how are you doing?
I give you my blessing. Now get cracking and do a proper rebuild.


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subset: commoncrawl


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We have developed chip-integrated photonic biosensors for applications in early cancer detection and environmental pollution detection. We have also developed mid-infrared lab-on-chip absorbance sensors for the detection of various gases. Both technologies are currently being packaged for commercialization.More
We are developing innovative photonic devices on silicon platform with an emphasis on reducing the size, weight, and power consumption of devices.More
roll-to-roll (R2R) manufacturing technology for developing hybrid electronic systems on flexible substratesMore
Utilizing nanoimprinting and ink-polymer jet printing to fabricate photonic devicesMore
The XtremeSens Diagnostics Platform is being developed to address medical and clinical diagnostic needs for biomolecule (protein, DNA, mRNA) detection with high sensitivity, high specificity, high multiplexing capability with minuscule sample volume.More
Omega Optics, Inc. was founded in 2001 to design and fabricate optical components based on the planar integrated polymer and silicon waveguide technology.


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This review is from: SUPERMICRO CSE-842TQ-665B Black 4U Rackmount Server Chassis 665W 3 External 5.25" Drive Bays
Pros: Solid construction
Well placed attach points.
Modular fans.
Cons: 5.25" drive bays could use a little more support on the modular snap-ins. Slight free-play noted under light pressure.
Other Thoughts: Great Server case, and the MCP-290-00058 short case rails are a good way to attach this to your rack.READ FULL REVIEW
This review is from: ASUS P8B WS LGA 1155 Intel C206 ATX Intel Xeon E3 Server/Workstation Motherboard
Pros: The P8B WS is a solid board that we run in arrayed networking for high demand graphics. Easy install and startup.
Cons: None
Other Thoughts: Auto detect Sandy/Ivy bridge. I run 75% sandy bridge and have to re-chip for the Ivy bridge upgrade. The P8z77 has a usb upgradeable bios, would be nice to see the C206 board with the same setup and avoid having to use a sandy bridge chip for the needed Ivy bridge bios upgrade if you purchase an Ivy bridge chip.
Definitely a solid performer, fast great graphics/networking board.
I run over 8 of these boards in different applications.
I ran into a bios issue on one board, and ASUS support handled the RMA very well.
Highly recommended.
This review is from: Corsair Obsidian Series 550D Black Aluminum / Steel ATX Mid Tower Computer Case
Pros: Nice solid constructed case.
All hardware included, everything fits well.
Nice quick mounts
Snap in mounts have mount locations for ssd/2.5 drives
Cons: N/A
Other Thoughts: Corsair makes a nice case that looks great. For high profile locations and personnel, the design is very well thought out.READ FULL REVIEW


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2011 Hyundai Equus Ultimate
This car makes us combine the words "60 grand" and "Hyundai" in the same sentence.
Road Test: 2012 Hyundai Azera
MotorWeek road test review of the 2012 Hyundai Azera. Hyundai is clearly not satisfied with being a major player in just the small and mid-size car and crossover ranks. They've also had success luxury segments with cars like Genesis and Equus. But, one area where success has eluded them is in the large family sedan. They've tried with the XG300 in 2001 and Azera starting in 2006. Still, sales were disappointing. But, Hyundai never says never, and for 2012 the Azera is all-new. So let's see if this time their efforts pay off big.
2012 Hyundai Azera Drive and Review
( http://www.TFLcar.com ) For 2012 Hyundai Azera is all new. This fill-sized sedan from Korea competes with cars like the Toyota Avalon, Ford Taurus, Chrysler 300 and perhaps even the Lexus ES 350. For a base price that starts around 30K, the 2012 Hyundai Azera offers a lot of value for the money. The Azera can be had with a massive sunroof, it comes standard with plenty of leg room in both the front and back and seats that are all day road trip comfy. However unlike some of its rivals the Azera is front wheel-drive and not the fastest and certainly not the sportiest car in the segment. Check out this first drive review as we put the new and Sonota like Azera to the test.
2016 Hyundai Azera - Review and Road Test
Boasting a stylish exterior and an abundance of premium features the latest Hyundai Azera is an appealing full-size sedan. In this video Kelley Blue Book's Micah Muzio drives the Azera and highlights a compelling alternative from an unlikely source. For the latest Hyundai Azera pricing, reviews and vehicle information: http://www.kbb.com/hyundai/azera/2012... Kelley Blue Book is your source for car reviews, road tests, new car launches & auto show videos. Subscribe to catch all the latest Kelley Blue Book video reviews. http://www.youtube.com/subscription_c... To follow Micah on Twitter, please visit: http://www.twitter.com/micahmuzio


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With Fall 2011 in full swing, SUPRA introduces two new boots: the Douglas and the Skyboot. The Douglas outdoor boot comes constructed with a waterproof, full-grain leather silhouette with heavy gauge stitching, tough metal eyelets and woven rope laces. The Skyboot is reminiscent of the popular Skytop sneaker and features heavyweight moccasin-style stitching along with rugged raw leather laces, eyelets and trim. The Skyboot also includes an internal "stash pocket" in the tongue to keep your valuables dry. Both fall boots are built with the durable Skywalk lug outsoles for ultimate traction and flexibility. Purchase the boots now at SUPRA retailers worldwide, including Suprafootwear.com.


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Forum:"1/2 A" & "1/8 A" airplanes
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Xian Sost Biological Science & Technology Co., Ltd.
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|Business Type:
|Trading Company
|Shaanxi, China (Mainland)
|Main Products:
|Plant and animal extract,Cosmetics raw materials,Health food raw material,fruit and vegetables extract,resveratrol 98%
|Total Employees:
|51 - 100 People
|Total Annual Revenue:
|US$2.5 Million - US$5 Million
|Year Established:
|Top 3 Markets:
|South Asia 7.69% Southern Europe 7.69% Northern Europe 7.69%
Xi'an Sost Biological Science & Technology Co., Ltd. relies on the geographical advantages of Mo...
Xi'an Sost Biological Science & Technology Co., Ltd. relies on the geographical advantages of Mountain Qinling in the west of China. With biological engineering and natural extraction technology as the foundation, we integrate the research and development, production and sales.
We have advanced extraction, separation, purification and drying equipment and quality testing equipment, such as HPLC, GC, AFS, UV, etc. We have management personnel with expertise and technical personnel to implement scientific operating systems in production flow supervision, quality inspection and other aspects. We carry out modern enterprise management methods. Our company is in full accordance with GMP standard of the state to establish and improve our production system and quality assurance system to adequately ensure the safety, efficacy and stability of our products.
We customize and develop new varieties according to customers' demands, combining with years of plant extract R&D experience and technical reserves, rapid development and trial production, as well as 2-3 day delivery.
Our products are exported to more than forty countries and regions, such as Europe, North America, South America, the Middle East, Southeast Asia, etc. The whole production process conforms to international standards so as to ensure product quality effectively, and we provide better service for global customers. Customer satisfaction is our long-term desire. We will make full use of our own advantages, being aggressive and innovative.
Trade Capacity View More >
Trade & Market
|Main Markets
|Total Revenue(%)
|South Asia
|Southern Europe
|Northern Europe
|Central America
|Western Europe
|Eastern Asia
|Southeast Asia
|Eastern Europe
|South America
|North America
|Export Percentage:
|41% - 50%
|Export Mode:
|Have Own Export License
|No. of Employees in Trade Department:
|11-20 People
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Makes about 2 dozen 1-1/2" fritters
Crunchy, sweet, and savory, these fritters spice up any occasion and, served with a ginger-flavored mustard for dipping, make an enticing before-dinner appetizer.
Mix together all the ingredients except the oil until they are just combined. Be careful not to over mix. In a deep frying pan, bring about 1-1/2" oil to 350 degrees and drop in the batter, spoonful by spoonful, being sure not to crowd the pan and allowing a few seconds between each fritter to allow the oil to return to the proper temperature. Turn the fritters after one minute and fry until they are golden brown, about one more minute. Using a slotted spoon, transfer the fritters to absorbant paper to drain. Continue until all the batter has been used. Serve the fritters immediately, with one or more of the suggested sauces.
Copyright 1996 by Michele Anna Jordan, author of the Good Cook's Book of Mustard. All Rights Reserved. Reprinted with permission.
Michele Anna Jordan's books, including The Good Cook's Series (on Mustard, Tomatoes and Oil & Vinegar) are available at bookstores nationwide, or they may be ordered from Katz and Company at 800-455-2305; fax 707-254-1846.
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Hard shell snap on case for iPhone 5 with bright pheasant feather design. It will protect your iPhone from marks and scratches, without impairing access to all phone functions or the acoustic performance.
Thank you for visiting Venom Pheasant Case for iPhone 5 & 5s on shoppingfind.co.uk
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|Document Type:
|All Authors / Contributors:
Margaret J Wheatley
|9781576757642 1576757641
|OCLC Number:
|Previous edition: 2002.
|182 pages : illustrations ; 24 cm.
|Turning to one another: Now is the time ; Welcome ; Why I wrote this book ; How to use this book ; Can we restore hope to the future? ; What I believe at this time ; Simple processes ; The courage of conversation ; The practice of conversation ; Willing to be disturbed --
A place to pause and reflect --
Conversation starters: Now it's your turn ; Do I feel a vocation to be fully human? ; What is my faith in the future? ; What do I believe about others? ; What am I willing to notice in my world? ; When have I experienced good listening? ; Am I willing to reclaim time to think? ; What is the relationship I want with the earth? ; What is my unique contribution to the whole? ; When have I experienced working for the common good? ; When do I experience sacred? ; What is our role in creating change? ; Can I be fearless? ; Gestures of love ; Turning to one another ; Referenced quotes ; About the author ; A story from the Aztec people.
|Series Title:
|BK life book.
|Other Titles:
|Simple conversations to restore hope to the future
|Margaret J. Wheatley.
"I believe we can change the world if we start talking to one another again." With this simple declaration, Margaret Wheatley proposes that people band together with their colleagues and friends to create the solutions for real social change, both locally and globally, that are so badly needed. Read more...
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Related Subjects:(9)
- Conversation.
- Communication in small groups.
- Group decision making -- Religious aspects.
- Social action.
- Cooperativeness.
- Life.
- Interpersonal Relations.
- Social Environment.
- Social Change.
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- DMIN 0911: cohort 4(48 items)
by tyndaleacq updated 2013-04-16
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by spederson updated 2011-09-28


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Interactive installation based on neuro-bio feedback loop using EPOC Emotiv Headsets. Concept, research, graphic design, interface design, installation and web design >> Saša Spačal Concept, programming, visualisation and sound design >> Tadej Droljc Concept, neuroscience coordinator, EEG analysis, project member >> Simon Brežan, dr.
A three minute 720p preview of a ten years and counting work-in-progress.
The Lorelei Suite comprises of three different applications, each one designed with a specific sound or compositional process in mind.
Shanghai Traces was a response to the massive beautification campaign that Shanghai underwent in preparation for hosting the World Expo in 2010.
"Backup Aura" is an album of electronic music consisting of six compositions that make use of the computer as a feedback tool for extending the ideas and intuitions of the composer.
Pivoted Limbs is an electronic music EP recorded at my home studio.


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Decision 1 for AQA
2nd Revised edition
By (author) Stan Dolan
Format: Paperback
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Decision 1 for AQA by Stan Dolan
Book DescriptionThese two books have been written specifically for the Decision 1 and and Decision 2 modules of the AQA Mathematics specification.
Buy Decision 1 for AQA book by Stan Dolan from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780521619165
(245mm x 190mm x 10mm)
Pages: 144
Imprint: Cambridge University Press
Publisher: Cambridge University Press
Publish Date: 16-Jun-2005
Country of Publication: United Kingdom
Books By Author Stan Dolan
AQA Mathematical Studies Student Book, Paperback (February 2016)
In full colour and written specifically for the AQA Level 3 Certificate in Mathematical Studies, this book provides plenty of worked examples, practice questions and practice exam papers. Set in engaging contexts relevant to a wide range of other post-16 subjects, AQA Mathematical Studies is also supported by online teacher notes.
Decision 2 for AQA, Paperback (June 2005)
Two books written specifically for the Decision 1 and and Decision 2 modules of the AQA Mathematics specification.
Decision 2 for OCR, Paperback (May 2005)
Fully endorsed by OCR for use with OCR Mathematics GCE specification
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Kathy, I, too, had never seen an oriole here at this time of the
year before. I can't imagine how or why he got here. Unfortunately,
I have not seen him again, though we have watched constantly.
I even put out half an orange for him, since I know they like fruit.
The weather was bitter cold for the next three days - just beginning
to warm up a bit now - so I fear he may have perished.
This morning is the first this week that hasn't been around 10 degrees.
However, it's predicted to rain later today - looks like it now. I know
from sad past experience that the rain will fall on the snow and
supercooled pavement and freeze into another sheet of ice. We've
just had too much of that this year. Oh well. At least the days are
getting noticeably longer. It's light when I get up at 6:00 AM instead
of staying dark until 7:00.
In a message dated 3/6/2009 3:10:25 AM Eastern Standard Time,
firstname.lastname@example.com writes:
The orioles are always an amazing sight but I can't believe you have one at
your feeder in the snow!! We have had a pair at our feeders the last couple
summers but they don't arrive until the end of April. I think our weather
here in IL is comparable to yours in the Hudson Valley. (I went to high
in Newburgh a very long time ago.) Kathy
In a message dated 3/2/2009 3:02:12 P.M. Central Standard Time,
email@example.com writes:
We have been having snow since sometime during the night - not
very heavy, but steady. It is now about seven inches deep, but
I'd far rather have snow than the freezing rain we had last month.
I just looked out the window and saw the most amazing sight.
A Baltimore (I know, they're Northern now, not Baltimore)
oriole. We see them now and then in the summer, but I have
never seen one here in the winter before. Brilliant against the
snow. The poor thing is acting starved - he's been steadily
eating at the feeder for more than ten minutes. The other
birds keep flying at him, but he just sits there and eats. I
don't know where he came from, but I'm glad he found the
feeder. NowI need to go out in the snow and fill it again - looks
as if its almost empty.
**************A Good Credit Score is 700 or Above. See yours in just 2 easy
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Hotels near Pertuis Station
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- Most bookedInter-Hotel Le Village Provençal
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"We stayed in the hotel for one night and enjoyed our time. There is a lovely courtyard with a water feature where we spent evening.The room was spacious and nicely decorated. "
A Traveller, Oct 2015, AU
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"Overnight stop on way to Cannes. convenient location out of town, easy to find."
A Traveller, Sep 2015, GB
Hotels within 2 miles of Pertuis Station
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"Our room was huge by European standards. Needs new carpet but rest of accommodation was clean and comfortable. We were only there for the night and did not eat their breakfast. The buffet looked good. Grounds were nice to sit out and have coffee."
A Traveller, Jul 2015, US
Hotels within 3 miles of Pertuis Station
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"We were travelling through on our way from Nice to Avingnon and wanted to see the lavendar fields. This was a nice out of the way country hotel that was close enough to some of the other cities. We arrived and were greeted by a hostess who only spoke French but was very willing to try and communi..."
A Traveller, May 2015, IE
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- Les Chenes VertsNo Guest Reviews yet
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"The surrounding area of the hotel is stunning. We thoroughly enjoyed our stay in this family run hotel. It's incredibly peaceful and you sleep very well! We found the staff so welcoming and the food was good too. "
A Traveller, Oct 2016, GB
Hotels within 6 miles of Pertuis Station
13 people looked at this hotel in the last hour
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"A very nice hotel, with a great room for the family. The room had 4 beds (2 double, 2 single). Everything was clean and the staff was extremely friendly. * If you are expecting the typical American hotel room, this is not for you. It's a traditional local French hotel in a small town."
A Traveller, Aug 2015, US
- Villa La CosteNo Guest Reviews yet
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Hotels within 7 miles of Pertuis Station
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Former Puerto Rican governor punched, hospitalized after making...
There are 43 comments on the International Herald Tribune story from Feb 6, 2008, titled Former Puerto Rican governor punched, hospitalized after making.... In it, International Herald Tribune reports that:SAN JUAN, Puerto Rico : A former governor of Puerto Rico was punched in the face after making a comment about U.S. President George W. Bush while having dinner at a restaurant, police said Wednesday.
Join the discussion below, or Read more at International Herald Tribune.
Since: Dec 07
Loiza, Puerto Rico
#1 Feb 6, 2008
I wonder what comments were made!
Since: Oct 07
Location hidden
#2 Feb 6, 2008
Romero Barcelo always said he NEEDED that extra security. Only HE knew what he was likely to say. As a former banker I assure you one has to say something pretty insulting to get beaten and hospitalized by a banker.
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#3 Feb 6, 2008
Who is the Puerto Rican banker who punched former governor Carlos Romero Barcelo requiring him to go to the hospital? What did Romero say? Was the banker a Republican or a Democrat? A member of the New Progressive or Popular Democratic Party?
Carlos Romero Barcelo is a top notch lawyer. Can we bank on Carlos suing the banker? What a scandal!
rollingthunder you have a scoop here! Congratualations!
Since: Feb 08
Loiza, Puerto Rico
#4 Feb 6, 2008
I am traveling to Puerto Rico in April to visit a friend for the summer. She lives in Ceiba and said it is a safe place. Im trying to find out because I have read many articles concerning the crime rate and now reading about a former politician being assaulted rises some concerns. I heard that San Juan is ok to visit in the day time but not at night.
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#5 Feb 6, 2008
Who is the gentleman in the photo that accompanies this story? I know it is not Carlos Romero Barcelo unless he lost weight and had a face lift. Could it be a photo of rollingthunder?
Since: Feb 07
Vega Baja, Puerto Rico
#6 Feb 6, 2008
Since your ISP shows as Fajardo, I would assume you already have the answers to your questions.
Since: Dec 07
Loiza, Puerto Rico
#7 Feb 6, 2008
Very Observant on the Pic! He is Paulo Cohelo. I actually posted the pic to see if another person would notice the mistake and comment. After receiving a comment from Viroslaf Vitous or whatever he may call himself I was bound to actually post a topic. He had made a comment about my collquialisms and distintions in my writing format however never answered my question back. I dont mind being foolish at times however I believe he took me for a fool.
I believe we both know what group he belongs to!
Thanks on the Congrads!
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#8 Feb 6, 2008
What counts the most is that YOU DO NOT THINK THAT YOU ARE A FOOL. I would be very unhappy if I tried to lived my life on other people's terms rather than on my own terms. It is important that you like (love) yourself. Your friends will accept you if you are true to yourself.
Ignore the trolls .... You can never win with them. I know from experience, especially on Topix.
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#9 Feb 7, 2008
Believe it or not, Raymond Molina, who punched Carlos Romero Barcelo had participated that morning in a Senate ceremony recognizing several dozen businessmen selected by the San Juan Star for its Merit 2007 award.
It is understand that he's over seventy years of age.
There is a question about whether the comment was about Bush or had to do with the Bay of Pigs. I did not have my San Juan Star delivered yet this moring. I was hoping to read about it in the STAR.
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#10 Feb 7, 2008
Exactly who is Raymond Molina who punched Romero?
Did it have something to do with the "Bay of Pigs"?
Believe it or not, Raymond Molina, who punched Carlos Romero Barcelo had participated that morning in a Senate ceremony recognizing several dozen businessmen selected by the San Juan Star for its Merit 2007 award. It is understand that he's over seventy years of age. There is a question about whether the comment was about Bush or had to do with the Bay of Pigs.
Is the Raymond Molina, who punched Carlos Romero Barcelo, the same Raymond Molina mentioned in the July 23, 1998, story in the Miami Times or a relative?- Check link below...
Also, check out the link for "Bay of Pigs Vets Fight for Home - Betrayed by the U.S. government and their own country, they want to be remembered."By Janine Zeitlin Published: January 17, 2008 - Miami Times.
Quote from the 1998 Miami Times story:
"...Several creditors are also searching for the enigmatic Mr. Molina. He failed to pay the last month's rent,$700, for his campaign office at 3383 NW Seventh St., according to building manager Armando Rodriguez: "He told me he would come back to pay but he never did." Eladio Jose Armesto, publisher of the Hispanic weekly El Nuevo Patria, said Molina owes him $3000 for advertising and consulting: "He made promises that he would pay me next week, then the week after, then he would tell me that
his brother in Puerto Rico was sending him some money.
My attorney wrote him warning him that we might take it to court, but we are still waiting..."
http://miaminewtimes.com/1998-07-23/news/raym... Miami Times 1998 story...
http://miaminewtimes.com/2008-01-17/news/bay-... Miami Times 2008 story...
#11 Feb 7, 2008
Bravo for Molina!!! This Romero Barcelo is one of the most arrogant, self imposed, bully of teh PNP Party.....
Rivera Schatz & "The Horse" are known bullies and the Sopranos of Puerto Rico....what the hell did you think did they do to Ex Senator of Arecibo Loubriel to make way for Rossello to "assume" his position...
It was conducted in the back room with a 500 watt lite bulb right over his head and the guys were having a field day with their batons...
The the famouns Cero Marvilla Speech on July 25, l978 claiming the murderers of those two jerks were Terrorists and proclaimed the Cops as Heros.....it was a freaking television tower....
They surrendered peacefully and yet were shot down like a bunch of stray dogs......Romero Barcelo & Hernandez Colon in their 20 years in Fortaleza have divided this Island into a trech war fare and never recovered.......
They were excellently replaced with the likes of Rossello, Sila Calderon adn this Bimbo Acevedo Vila......Puerto Rico will never have peace in this Century....
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#12 Feb 7, 2008
We live in a nation of laws. Because we do not agree with someone, we do not have a right to punch them brutally in the eye and cause serious bodily injury and suffering. Almost every citizen accepts this reality. It is basic to law and order and a democratic society.
How can someone of reasonable intelligence say "bravo" to another person hitting someone because they do not agree with them? Is this the kind of criminal behavior that he teaches his grandson? As a German Jew living under Hitler, how can he endorse brutality of one person against another? Did he not learn from his experience of living in Germany under Hitler?
Carolo Romero Barcelo will hit Raymond Molino with a enormous law suit and will win. I hope he sues Molino for millions. He will win.
Shame on a German Jew endorsing brutality and not following the law. What motivates this kind of undemocratic thinking... stupidity or hate...?
#13 Feb 7, 2008
You are a real winner....Romero Barcelo is a bully, he is a henchman...he has no freaking honor and once again you bring up the German Jew Crap with Hitler....you must think I am l25 years old...
I left Germany at 3 years old to Palermo, Italy due to the wisdom of my Grandfather and eventually got passageway to the United States via Porutgal.....
What the freaking f**k are you talking about as I am a German Jew with Hitler??? You are missing your memory pills once again and stop this charade of "PERSOAL ATTACKS" that you so conveniently condemned of others and you go right ahead and do the same...
Go down to Vendig Street by Walgreens and get a quick job to quiet you down....you are really getting obnoxious and I hate and despise Romero Barcelo, Hernandez Colon, Pedro Rossello, Sila Calderon and this Bimbo Acevedo Vila....
That's five straight Governors of this Island that haven't done jack shit for this Island...I don't give a crap about political parties on this Island....
My grandsons speak english, spanish and german at 5-9 years old....how are ya doing???
#14 Feb 7, 2008
You don't know jack crap about the incident....you ahve no clue if Romero Barcelo verbally abused this Molina Guy....you have no clue if the "sucker punch" was defesnive....you have no clue about anything but you eat up the newspapers media and your blind faith for this political party that is full of Sopranos...
Osvaldo Gil, US District Attorney said it right and appropriately....there is a Name for Corruption in Puerto Rico and it's the PNP Party.....
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#15 Feb 7, 2008
Did your gay brother tell you about Vendig Street? Do you hate him or think he is inferior because he is gay? When was the last time you talked about civil rights with him?
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#16 Feb 7, 2008
Did your gay brother tell you about Vendig Street? Do you hate him or think he is inferior because he is gay? When was the last time you talked about civil rights with him?
#17 Feb 7, 2008
What is this nonsense all about??? My Gay Brother and Vendig Street.....what's the connection??? Why do you always look for the slime and greasy replies of your lonely and scorned life as a failed journalist and an even failed homosexual....
What's the big deal about my Personal Life??? Why do you always brins up attacks on my family and others like David Lopez who disagrees with some of you bullshit postings...
Then you retort with this Gay Brother nonsense that has nothing todo with Puerto Rico, Romero Barcelo or this Cuban Guy named Molina....
I stated an opinion and you come out blasting against my family and my brother like it's your business.....
I never talk about your personal life in New Hyde Park or your Family so quit this bullshit and come face to gface like a Man...oops....there I go again with a dream!!!
You ungrateful ugly American....you are the ones that the puertorriquenos despises so much behind your gayish back....
"Did you tell your Brother about Vendig Street" What the F**K is this all about??? You filthy mouth scumbag
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#18 Feb 7, 2008
"Harry Craig" of Topix -- check out your postings. You are the one that always gets personal. You brought up Vendig Street and the Gay stuff on this thread. I did not. You can not argue the points of an issue. On post after post, you bring in that I am gay and want to go to Vendig.
You are a spoiler as most trolls are on a blog.
Before you started posting on this thread, I never once mentioned or even hinted at being gay.
Then Topix's Harry Craig --- the one always using the word "crap" --- starts posting. Does he discuss the issues of the topic? No he calls me a "woman" -- brings in Vendig, then one personal inaccurate attack after another...etc., etc., etc. Howver, you are too blind to see yourself for what you really are.
Then, you say I took control of Topix. We were doing quite nicely in our discussions until you arrived with your stupidity, prejudices, and insults. You spoil every thread on Topix with your sickness.
Your brother is gay which means the gay gen is in your family. Careful ...you may discover that a younger generation has the gene and will come out of the closet during adulthood. It has happened many times...
By the way, Carlos Romero Barcelo is on a way higher plane of honor and respect than Topix's Resident Harry Craig... He would not want to be in the same room with Topix's Harry Craig
"Honorable Permanent Union"
Since: Aug 07
San Juan, Puerto Rico
#19 Feb 7, 2008
Former Puerto Rico Governor Carlos Romero-Barcelo
Born: September 4, 1932
Birth State: Puerto Rico
Party: New Progressive Party
Family: 4 children
Religion: Catholic
School(s): Yale University, University of Puerto Rico School of Law
Periods in Office: From: January 1, 1977
To: January 1, 1985
CARLOS ROMERO-BARCELO was born in San Juan, Puerto Rico, in 1932. He graduated from Yale University and the University of Puerto Rico School of Law. After establishing a career as a San Juan attorney, Romero-Barcelo entered politics in 1965. He was named president of a movement known as "Citizens for State 51." That group merged with others in 1967 to form United Statehooders, which evolved into the pro-statehood New Progressive Party. Romero-Barcelo was named the party's first candidate for mayor of San Juan in 1968, and won two four-year terms in that office. As mayor, Romero-Barcelo served on the board of directors of the U.S. Confernece of Mayors and in 1974 became the first and Hispanic-American elected to the presidency of the National League of Cities. In 1976, Romero-Barcelo was elected governor and he was reelected in 1980. The governor served on the President's Council on Education of Disadvantaged Children and chaired the Southern Governors' Association in 1980-81. He also served as secretary of the Democratic Governors' Conference. After his term as governor, Romero-Barcelo served in Congress, representing 3.7 million American citizens as the Resident Commissioner in the 106th U.S. Congress for the territory of Puerto Rico
#20 Feb 7, 2008
For those who do know Spanish and not willing to learn..I posted this in two languages for the ugly tourists in Puerto Rico for over 20 years....
Esta es otra version de La Primera Hora de hoy sobre el incidente de Cubano Raymond Molina and Gobernor Romero Barcelo en el restaurante Pelayo's de Condado...
El ex gobernador Carlos Romero Barceló tendrá que ser sometido a por lo menos una intervención quirúrgica tras recibir un puñetazo en el rostro en medio de una discusión sobre política estadounidense en el restaurante Pelayo del Condado la noche del martes.
Ex Governor of Puerto Rico, Carlos Romero Barcelo, underwent surgery at San Jorge Hospital after receiving a "sucker punch" in the face causing injuries to his nose, eyes and his forehead.....he was wearing eyeglasses during the incident.
Le rompió los espejuelos y se le incrustaron los vidrios dentro del ojo, tiene daños dentro del ojo, el hueso que aguanta el ojo está roto y tiene la nariz rota también, manifestó Melinda Romero (Hija) a Prensa Asociada.
The breaking of the breaking and shattering of the eyeglasses left residues of glass in one of his eye and suffered a broken nose stated Melinda Romero to the Associated Press....
Informaciones periodísticas apuntan a que el hombre se molestó por unos comentarios que el ex gobernador dijo sobre el presidente estadounidense George W. Bush.
The person identified as Raymond Molina who was visibly upset over comments made by Romero Barcelo on President George W. Bush..
El superintendente de la Policía, Pedro Toledo, dijo que contra el agresor, identificado como un banquero, aún no se han radicado cargos."Hoy será entrevistado", sostuvo a una emisora radial (Radio-Isla).
Police Superintendent Pedro Toledo identified the aggresor as only a Banker and there was no charges filed at this time.
Detalló que en medio de la discusión, el hombre le hizo un gesto a Romero Barceló como recriminándole y el ex comisionado residente le agarra la manos, procediendo el hombre a zafarse y lanzarle el puño.
Certain details of the incident revealed that Molina made a gesture towards Romero Barcelo that was incriminating and Romero and afterwards, Molina threw the "sucker punch".
Líderes del Partido Nuevo Progresista (PNP) catalogaron hoy de "lamentable" el puñetazo en la cara que recibió el ex gobernador.
Para Kenneth McClintock, la agresión al ex gobernador es evidencia de que "somos un pueblo demasiado violento".
The President of the Senate, Kenneth McClintock that the agression on the Ex Governor was just another example of how violent this Society has become...
"En Puerto Rico tenemos la tendencia de que, en vez de pegar el freno, tocamos la bocina, de que a la menor provocación tiramos el puño en vez de darle la espalda a las personas que entendemos que nos están faltando el respeto", expresó.
McClintock added that we have the tendency instead of avoiding incident and "putting on the brakes", any incident no matter how insignificant, we resort to violence instead of turning away and avoiding any disrespect.
Themas políticos, aunque sean estatales o nacionales, tienden a ser demasiados apasionados, pero uno siempre tiene que tener la oportunidad y el espacio para aguantarse y no llegar a un punto de agresión física", manifestó.
Political themes and arguments whether National or Local, this Society have engulfed themselves in a passionate aggression and gives no space to avoid incidents and ultimately, become violent and physical aggression..
Yes...we are a Nation of Laws!!!
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Resentment leads to a fight, and shortly after former friend Paul is badly injured in a road accident. A protracted journey into the Absolute Elsewhere enables Chris to cheat time and save his friend's life, but by now the story has gone on too long for its own good. Before that, some authentic dialogue and a lively adolescent eye for adult foibles makes this an entertaining and sometimes moving read.
Robert Cormier is still one of the more controversial authors writing for teenagers, even though his first novel came out in 1974. Shining a powerful literary torch into dark areas many would prefer the young to leave unexplored has some merit, but the danger of sensationalism for its own sake is also always near. His latest work Tenderness (Gollancz, pounds 12.99) is more a cut-down adult story than a novel written to expand immature understanding. The inner life of an 18-year-old psychopathic serial killer makes for a gripping but ultimately nasty read, with his final conversion by the 12-year-old runaway he plans to kill both unconvincing and unintegrated into the pessimism of the tale. In his time Cormier played a useful part in challenging the unwritten law that all stories for children should end, if not happily, then at least on a note of moral resolution. Tenderness, with its absurd coincidences, over-explicit character descriptions and uneasy vocabulary of growing sexual awareness, is a shadow of his previous work.
Elizabeth Arnold's Gold and Silver Water (Mammoth, pounds 4.99) is a much more up-beat affair. This is the sequel to her successful The Parsley Parcel, and describes more adventures of Freya, a gypsy girl with the power of second sight. Her task this time is to restore a small child traumatised into mutism after witnessing the death of her mother and sister. Freya herself communicates in Gerard Manley Hopkins style (sometimes she feels "weary-world worried" or "heart-hungry home-sick"). This unusual, genuinely original piece of writing has the added luxury, for today, of a happy ending.
Lynne Markham's The Closing March (Mammoth pounds 4.50) is also a positive story, though set in a town where the young hero's grandfather and the pit he used to work in are both approaching extinction. Mick decides he would like to take his grandfather's place in the doomed colliery's final march, playing the old man's cornet at the same time. And that more or less is what happens, but only after a well-constructed plot where the pros and cons of mining as a way of life get an honest airing. The author occasionally becomes over-didactic in her eagerness that no reader should miss any of the points involved in Mick's occasional misgivings about life, death and art, where her real talents lie. But teenagers who enjoyed the film Brassed Off might also want to read this fictional counterpart to it.
Chris Westwood looks to the future in Virtual World (Viking, pounds 10.99), which offers readers a dizzy ride into the menacing potential of the type of "infotainment" offered by virtual reality and the Internet. A crazed inventor markets a game that traps viewers into giving up their own individuality. Once inside, it is hard to get out; once outside, there is an overwhelming urge to return. Terry Pratchett has already explored these intriguing possibilities in his stories, more successfully than Westwood in that he creates better characters while making much funnier jokes. Games-freaks may still go for this story, however, attracted by its general enthusiasm for the new worlds we are now creating.
Hugh Scott's The Ghosts of Raven's Crag (Walker Books, pounds 8.99) features a self-congratulatory family where everyone laughs loudly at each other's facetious remarks. So when a local evil spirit intervenes it is hard to be concerned, especially if ghastly, ever good-humoured Dad ("Little perishers!") is going to get some sort of come-uppance. But he finally wins through in a climax where the moans and shrieks, the dull thuds of a dead horse being soundly flogged are also evident. What a disappointment from an author with such a good track record.Reuse content


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Par 3
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This property is conveniently situated in Oliva . Hotel was constructed in 2007. The Hotel boasts 8 apartments. The Hotel is ideally situated in the city centre. The Hotel is within easy reach of the city's main bus and train stations. The Hotel s proximity to the metro and bus stops will allow guests to explore the area. The Hotel is in the vicinity of a local golf course. The beach is only a few metres away from the Hotel.
Avda Matisse, 3, Oliva Oliva 46730, Spain


text: What's $25 Million Between Friends?Leitch5/17/06 12:45pmFiled to: EspnESPN Mobile0EditPromoteShare to KinjaToggle Conversation toolsGo to permalink This image was lost some time after publication, but you can still view it here. And you thought we were unduly picking on ESPN for the ESPN Mobile fiasco. Advertisement From yesterday's Sports Business Daily:Mobile ESPN has lost $25M so far after launching a $30M ad campaign that included an ad on ABC's Super Bowl broadcast, according to Alice Cuneo of AD AGE. Disney President & CEO Robert Iger acknowledged, "Initial results were a little bit lower than we had hoped. But we have changed our pricing approach, we have strengthened and redirected our marketing, we're expanding our presence at retail." Ovum wireless analyst Roger Entner said that the $25M loss "would be considered 'normal' by carrier standards." Entner added, "I would rather expect that to increase with more customers coming on board, but to swing into profitability [within 2-3 years]"This, of course, makes total sense, because a technology that already seemed outdated (not to mention, of course, unreasonably expensive) the day it came out should absolutely come around to profitability in two or three years. Technology tends to be nice enough not to change for large corporations looking to over-leverage a brand name. Advertisement Wait ... does this mean we have to watch those ads for the next two years?Sports Business Daily [Official Site]ReplyLeave a reply


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Blade Helis Tail Rotor Blade Set
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Michael Hanson
Smashwords Edition
Copyright © 2009 Michael Hanson
All rights reserved.
E-book ISBN 978-0-9866697-1-2
Discover other titles by Michael Hanson at Smashwords.com https://www.smashwords.com/profile/view/michaelhanson
Smashwords Edition, License Notes
This ebook is licensed for your personal enjoyment only. This ebook may not be re-sold or given away to other people. If you would like to share this book with another person, please purchase an additional copy for each person you share it with. If you're reading this book and did not purchase it, or it was not purchased for your use only, then you should return to Smashwords.com and purchase your own copy. Thank you for respecting the hard work of this author.
This is a work of fiction. Names, characters, places and incidents are products of the author's imagination or are used fictitiously and are not to be construed as real. Any resemblance to actual events, locales, organisations or persons, living or dead is entirely coincidental.


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Huge fan too and member of another board dedicated to them too.... MMDs are the awesome! They even have a Band Based on Emerson, Lake and Palmer called MRH.. Miku, Rin, and H@chune! :-)
post #16 of 18
5/10/12 at 3:47pm
- 287 Posts. Joined 7/2005
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text: Arts & Crafts | Michaels Stores-40% Off One Item Sale See It! About this Deal Take 40% Off Any One Regular Priced Item. Ends 6/29/13. Spend $25 get $5 off valid through june 22 Flag this deal Edit deal What's the matter? Choose a reason Dead deal Duplicate Bad link Spam Inaccurate No value Alive again Comments


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text: Down On The Street: Cimarron d'Oro!Murilee Martin5/15/07 10:30amFiled to: GM Mistake Still A-Hauntin' Us1984Cadillaccimarron18EditPromoteShare to KinjaToggle Conversation toolsGo to permalink After spotting the super-awesome Opera Light Van the other day, I've been keeping my weather eye open (and low-end cameraphone ready) for similar finds: parked vehicles that look mundane at first glance but reveal themselves to be something much more upon closer inspection. Likesay this '84 Cadillac Cimarron d'Oro... See, GM figured that $12,964 ($26,109 in 2007 dollars) might seem like a lot to spend on a rebadged Cavalier, so they went the extra mile and added gold trim to the Cimarron, creating the incredibly classy Cimarron d'Oro Edition. And here's one that's still carrying the Cimarron flag high! Advertisement Advertisement 1984 CADILLAC CIMARRON [100megsforfree.com]Related: Short-Lived GM Car of the Day: Cadillac Cimarron [internal] Reply18 repliesLeave a reply


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Started by
Alexandra, June 13, 2002
Posted June 13, 2002
Anyone else go?
I did. ;) I'd say it was a run-of-the-mill performance, equal to ABT on a non-star night. Anna Antonicheva appears to be a "house ballerina" (a more tactful way of saying utility ballerina)—competent but nothing special. It's hard to enjoy her performance when she looks so pained; I don't think she was injured, the expression seems to be habitual with her. (See the head shot of her on Marc Haegeman's site.) Both she and Andrei Uvarov were much freer and more relaxed than at the opening gala, and I warmed to Uvarov a bit more than I had on opening night.
The "twin" Marias, Alexandrova and Allash, danced the pas de trois (with Uvarov) and two of the national dances in Act III. Two years ago, Alexandrova looked poised to become the Bolshoi's new star, but here she is, back in soloist roles again. She will get to dance Gamzatti on Friday, however.
Grigorovich's production has a new, puzzling ending: Odette bourees behind a scrim and dies, Siegfried suffers silently centerstage. At least in other versions they get to be together, whether in death or in life.
It's strange to see the Bolshoi like this. In the past, they always elicited some kind of feeling, be it positive or negative, but never before have they been boring.
Did anyone else go? Is the Pope Polish? Here's my quick take, working from memory, so apologies if I omit the names of some soloists.
June 12, 2002
Bolshoi Ballet's 'Swan Lake'
Yuri Grigorovich's revised version, 2001
A far more satisfying night than the opening gala, not only because it was a full ballet, but because the overall level of dancing, especially by the corps, was on a higher level...not great, mind you, just 'higher'!
Changes to this version, compared to early Grigorovich -
Comparing what I know from the videos of the earlier Grigorovich version (starring Bessmertnova in one; Mikhalchenko in another)the most obvious change is the new, not-so-happy finale to the story -- Rothbart takes Odette down to sea with him, after she bourees to him behind a screen, leaving a sad Siegfried on stage alone. Grigorovich has also heightened the role of Rothbart a bit, particularly at the start of Act II, before the entrance of Odette; Rothbart partners Siefried and even lifts him a time or two. Last night's Rothbart, Dmitri Belogolobtsev, is an overall stronger dancer than the Siegfried, Andrei Uvarov, and the additions/extra-zippiness to the role of Rothbart seem tailor-made for Belogolobtsev. (A long essay on this revival, in the May/June 2001 issue of the Russian magazine 'Balet' seems to support this theory...Rothbart-Belogolobtsev was Grigorovich's true inspiration this time around.)
All Moscow & Mother Russia cheered the return of this production last year...just as it cheered the return of the Soviet national anthem, with revised wording, around the same time! If you had seen the Vasiliev version of 'Swan Lake' that this one replaced, then you would well understand what all the cheering was about when the Grigorovich returned. "All is now well in the Land of Oz, amen"...such was the feeling.
Last night's performance - the dancing:
Belogolobtsev was wonderful, especially in lightning-paced jetes & in his menacing solo of pirouettes a la seconde in the ballroom scene, surrounded by eight black swans.
Hurrah! -- the Jester lives! Yes, the Jester from the earlier production remains and, why not, given the glorious zingy dancing of the short Japanese dancer who essayed the role (M. Ibata, I think, is his name). The audience sprung to life after his first cavorting in Act I; huge volley of 'bravos' for this might-mite jester!
Anna Antonicheva must have gotten over her jet lag, as she was quite lovely & stronger last night, especially as the swan queen Odette. [but who advised her on her make-up for the white acts - frosty-white shadow on her eyelids & pale lipstick -- looked like a cadaver in motion. In contrast, her Odile makeup was gorgeous; no wonder Siegfried fell for the black swan!] Antonicheva's black swan was OK until the 32 fouettes which, as in the gala, were weak. So she is back on tonight for Nikiya...hmmmm...good luck to her.
As Siegried, Andrei Uvarov was an elegant gentleman....but no match for so powerful a Rothbart as Belogolobsev.
One of my long-time favorite features of the Grigorovich version of 'Swan Lake' are the five national dances performed by the fiancee-princesses on pointe, with respective 'platoons' of corps dancers! Last night's five princesses were all lovely:
Hungarian - Maria Allash
Russian - Olga Suvorova
Neapolitan - Nina Kaptsova
Spanish - Maria Alexandrova (those feather-light jetes of hers!)
Polish (Mazurka) - Mariana Ryzhkina ??
The corps of swans were lovely. They must be rehearsed day-in/day-out in this ballet & the near-perfection shows, especially in the legs. It's too bad that the hands & arms seem to go every which way.
Similar sloppiness was displayed during the palace scenes by a group of male corps members portraying "trumpet players" -- instruments so long, they look like muskets -- and those trumpet-muskets went every which way at times, instead of switching left-to-right in unison. Either do it precisely or keep the darn trumpets in one position, guys!!!
Sets & Costumes -
These remain as in the earlier Grigorovich. They are the work of Simon Virsaladze, who designed most of Grigorovich's ballets for three decades. The costumes remain stunning as ever. On the other hand, the heavy-metallic/glittery sets date the production...it's 'That 70's Show'!!!!
Jeannie Szoradi
Washington, DC


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1878 – Proposed Cathedral at Truro, Cornwall
Construction began in 1880 to a design by the architect John Loughborough Pearson, after an architectural competition.
Construction began in 1880 to a design by the architect John Loughborough Pearson, after an architectural competition.
Designed in a French Gothic style and built in 1878, the Dominican church is finished in rusticated limestone.
Most large Irish towns have a fine classical courthouse (Carlow, Monaghan, Dundalk), but Sligo's is a fine Gothic Revival ediface.
Offices of Gresham Life Assurance Society built on the site of St. Mildred's church which was sold and demolished in 1872.
The five storey Montreal City Hall is the work of architects Henri-Maurice Perrault and Alexander Cowper Hutchison,
Located in a quiet residential neighbourhood close to Parliament Hill, Laurier House is a National Historic Site. It was built in 1878 in the Second Empire Style,
A Victorian artists' colony in this part of Kensington with artists such as G.F. Watts and Luke Fildes,
From The Architect, September 25, 1875: "In the autumn of last year it may be remembered designs were invited by the governors of Roan's Charity Greenwich from six selected architects in limited competition,
Irish architect W.H. Lynn was brought over to Quebec in 1875 by the Governor General, Lord Dufferin,


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"I got ripped off. I can't believe they took away the two (points). You aren't supposed to reverse the call after you make one. It just didn't go my way."
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"[The storm, called Beta, sent relentless heavy rains over the region, ripping off roofs, toppling trees and flooding neighborhoods. Heavy rain in Honduras caused four rivers to overflow and damaged crops.] We are facing serious danger from the rain, ... We are staying on alert in nearly every part of the nation."
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Mifsud J.,The Medical Research Council
Molecular membrane biology | Year: 2013
The mitochondrial ADP/ATP carrier imports ADP from the cytosol into the mitochondrial matrix for its conversion to ATP by ATP synthase and exports ATP out of the mitochondrion to replenish the eukaryotic cell with chemical energy. Here the substrate specificity of the human mitochondrial ADP/ATP carrier AAC1 was determined by two different approaches. In the first the protein was functionally expressed in Escherichia coli membranes as a fusion protein with maltose binding protein and the effect of excess of unlabeled compounds on the uptake of [(32)P]-ATP was measured. In the second approach the protein was expressed in the cytoplasmic membrane of Lactococcus lactis. The uptake of [(14)C]-ADP in whole cells was measured in the presence of excess of unlabeled compounds and in fused membrane vesicles loaded with unlabeled compounds to demonstrate their transport. A large number of nucleotides were tested, but only ADP and ATP are suitable substrates for human AAC1, demonstrating a very narrow specificity. Next we tried to understand the molecular basis of this specificity by carrying out molecular-dynamics simulations with selected nucleotides, which were placed at the entrance of the central cavity. The binding of the phosphate groups of guanine and adenine nucleotides is similar, yet there is a low probability for the base moiety to be bound, likely to be rooted in the greater polarity of guanine compared to adenine. AMP is unlikely to engage fully with all contact points of the substrate binding site, suggesting that it cannot trigger translocation. Source
Mitochondrial carrier homolog 2 (MTCH2): The recruitment and evolution of a mitochondrial carrier protein to a critical player in apoptosis
Robinson A.J.,The Medical Research Council |
Kunji E.R.S.,The Medical Research Council |
Gross A.,Weizmann Institute of Science
Experimental Cell Research | Year: 2012
Recent studies report mitochondrial carrier homolog 2 (MTCH2) as a novel and uncharacterized protein that acts as a receptor-like protein for the truncated BH3-interacting domain death agonist (tBID) protein in the outer membrane of mitochondria. These studies, using mouse embryonic stem cells and fibroblasts as well as mice with a conditional knockout of . MTCH2 in the liver, showed that deletion of . MTCH2 hindered recruitment of tBID to the mitochondria with subsequent reductions in the activation of pro-apoptotic proteins, mitochondrial outer membrane permeabilization and apoptosis. Sequence analysis shows that MTCH2 is present in all examined multicellular Metazoa as well as unicellular Choanoflagellata, and is a highly derived member of the mitochondrial carrier family. Mitochondrial carriers are monomeric transport proteins that are usually found in the inner mitochondrial membrane, where they exchange small substrates between the mitochondrial matrix and intermembrane space. There are extensive differences between the protein sequences of MTCH2 and other mitochondrial carriers that may explain the ability of MTCH2 to associate with tBID and thus its role in apoptosis. We review the experimental evidence for the role of MTCH2 in apoptosis and suggest that the original transport function of the ancestral MTCH2 mitochondrial carrier has been co-opted by the apoptotic machinery to provide a receptor and signaling mechanism. © 2012 Elsevier Inc. Source
Kunji E.R.S.,The Medical Research Council |
Crichton P.G.,The Medical Research Council
Biochimica et Biophysica Acta - Bioenergetics | Year: 2010
Mitochondrial carriers link biochemical pathways in the mitochondrial matrix and cytosol by transporting metabolites, inorganic ions, nucleotides and cofactors across the mitochondrial inner membrane. Uncoupling proteins that dissipate the proton electrochemical gradient also belong to this protein family. For almost 35. years the general consensus has been that mitochondrial carriers are dimeric in structure and function. This view was based on data from inhibitor binding studies, small-angle neutron scattering, electron microscopy, differential tagging/affinity chromatography, size-exclusion chromatography, analytical ultracentrifugation, native gel electrophoresis, cross-linking experiments, tandem-fusions, negative dominance studies and mutagenesis. However, the structural folds of the ADP/ATP carriers were found to be monomeric, lacking obvious dimerisation interfaces. Subsequently, the yeast ADP/ATP carrier was demonstrated to function as a monomer. Here, we revisit the data that have been published in support of a dimeric state of mitochondrial carriers. Our analysis shows that when critical factors are taken into account, the monomer is the only plausible functional form of mitochondrial carriers. We propose a transport model based on the monomer, in which access to a single substrate binding site is controlled by two flanking salt bridge networks, explaining uniport and strict exchange of substrates. © 2010 Elsevier B.V. Source
Kunji E.R.S.,The Medical Research Council |
Robinson A.J.,The Medical Research Council
Current Opinion in Structural Biology | Year: 2010
Members of the mitochondrial carrier family are involved in transporting keto acids, amino acids, nucleotides, inorganic ions and co-factors across the mitochondrial inner membrane. The transporters are thought to share the same structural fold, which consists of six trans-membrane α-helices and three matrix helices, arranged with threefold pseudo-symmetry. During the transport cycle two salt bridge networks on either side of the central cavity might regulate access to a single substrate binding site in an alternating fashion. In the case of proton-substrate symporters the substrate binding sites contain also negatively charged residues that are proposed to be involved in proton transport. © 2010 Elsevier Ltd. Source
A metabolic model of the mitochondrion and its use in modelling diseases of the tricarboxylic acid cycle
Smith A.C.,The Medical Research Council |
Robinson A.J.,The Medical Research Council
BMC Systems Biology | Year: 2011
Mitochondria are a vital component of eukaryotic cells and their dysfunction is implicated in a large number of metabolic, degenerative and age-related human diseases. The mechanism or these disorders can be difficult to elucidate due to the inherent complexity of mitochondrial metabolism. To understand how mitochondrial metabolic dysfunction contributes to these diseases, a metabolic model of a human heart mitochondrion was created.Results: A new model of mitochondrial metabolism was built on the principle of metabolite availability using MitoMiner, a mitochondrial proteomics database, to evaluate the subcellular localisation of reactions that have evidence for mitochondrial localisation. Extensive curation and manual refinement was used to create a model called iAS253, containing 253 reactions, 245 metabolites and 89 transport steps across the inner mitochondrial membrane. To demonstrate the predictive abilities of the model, flux balance analysis was used to calculate metabolite fluxes under normal conditions and to simulate three metabolic disorders that affect the TCA cycle: fumarase deficiency, succinate dehydrogenase deficiency and α-ketoglutarate dehydrogenase deficiency.Conclusion: The results of simulations using the new model corresponded closely with phenotypic data under normal conditions and provided insight into the complicated and unintuitive phenotypes of the three disorders, including the effect of interventions that may be of therapeutic benefit, such as low glucose diets or amino acid supplements. The model offers the ability to investigate other mitochondrial disorders and can provide the framework for the integration of experimental data in future studies. © 2011 Smith and Robinson; licensee BioMed Central Ltd. Source