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Sea Gypsy ~ Puffer Fish Paradise Beach Rental
From $86 / night
|Per night:
|$100 - $170
|Per week:
|$600 - $1,000
|Pets allowed:
Bedrooms: 1
Bathrooms: 2
Sleeps: 4
Type: Condo
Pets allowed: No
Internet: Yes
Enjoy a fully equipped kitchen, new full sized appliances with new dishes, pots and pans. Enjoy your meals at the eating bar.
The living room features up-dated furniture, DVD player, cable, flat screen TV and wireless internet. Enjoy a large oversize slider with beautiful ocean front views and easy beach access just steps away.
This unit is close to the indoor pool and dry sauna with in unit towels for your use. There are beach towels for your use during your stay, please bring beach toys for your little ones.
Thank you for your interest and we hope to hear from you.
A short drive south down Hwy 101 takes you to Newport for deep sea fishing, whale watching, a visit to the Oregon Coast Aquarium and ever-popular Rogue Brewery. Newport's Historic Bayfront is a popular destination offering galleries, shops, restaurants and local special events to enjoy. The drive south provides many breath taking view points and photo opportunities with Depoe Bay also being a popular visitor location.
Depoe Bay is home to The Depoe Bay Whale Center and the highly acclaimed "Whale Watching Spoken Here" program. The center is dedicated to sharing information about whales and helping visitors to understand and appreciate these amazing creatures. Depoe bay has a pod of Grey Whales that are present 10 months of the year , you can charter a whale watching boat or try your luck with binoculars at the visitor center to see if you can catch them dancing in the distance. Depoe Bay isn't limited to just whale watching, you can also charter fishing boats and enjoy their local shops and dining.
Keep your eyes open along your drive for some of our restored light houses; many of which are open to visitors.
If you are new to Oregon it doesn't get much better than the Central Oregon coast; enjoy!
About the owner
Owner Profile
Availability Calendar
|Oct 01 2016
|Apr 30 2017
|2 Nights
|May 01 2017
|Sep 30 2017
|2 Nights
|Check In: 3 p.m. Check Out: 11 a.m.
|Lodging tax: 11.3%
|Deposits: A non-refundable deposit is due at the time of booking. The remaining balance will be charged no more than three business days prior to arrival date. Must be 25 years old to rent. Rates indicated are for current year only and are subject to change. No smoking and No pets
|Payment Options: Discover, Mastercard, Visa
|Other Fees: Cleaning fee of $50.00.
- 1 Queen Bed
- 1 Sofa Bed
- Beach Towels
- High Speed Internet
- Parking Included
- View (Ocean)
- Full Kitchen
- Linens Provided
- Private Pool
- Waterfront
- Heating
- Oceanfront
- Sauna
- Alarm Clock
- Ceiling Fan
- Microwave
- Stove
- Blender
- Coffee Maker
- Oven
- TV (Cable/Satellite)
- CD Player
- DVD Player
- Refrigerator (Full)
- Toaster
- Beach
- Playground
(<1 mile)
- Movie Theater
(1 mile)
- Horseback Riding
(5 miles)
- Ocean
- Restaurant
(<1 mile)
- Shopping Area
(1 mile)
- Whitewater Rafting
(5 miles)
- Pool
- Whale Watching
(<1 mile)
- Surfing
(1 mile)
- Hiking
(6 miles)
- Basketball Court
(<1 mile)
- Bicycling
(1 mile)
- Casino
(2 miles)
- Winery
(6 miles)
- Bowling
(<1 mile)
- Boating
(1 mile)
- Lake
(2 miles)
- Deep Sea Fishing
(12 miles)
- Fishing
(<1 mile)
- Golf
(1 mile)
- Live Entertainment
(2 miles)
- Aquarium
(22 miles)
- Grocery Store
(<1 mile)
- Gym/Fitness Center
(1 mile)
- Water Skiing
(2 miles)
- Scuba Diving
(22 miles)
- Park
(<1 mile)
- Miniature Golf
(1 mile)
- Windsurfing
(2 miles)
Reviews (12)
Reviews for this vacation condo rental in Lincoln City, Oregon
Absolutely wonderful condo!
Mary Catalano
Gresham, Oregon
Absolutely wonderful condo!
September 18, 2016
From the first point of contact with the onwer Adrianne until we checked out she was amazing! This condo is awesome from the view & beach access to the amenities and set up! I've never felt so at home staying in a hotel/condo! You can see that the owner takes pride in the property! So if your looking for an amazing rental at a great price I recommend The Puffer Fish Paradise!
Girls weekend-Great place had a wonderful time
Carol Heard
Portland Oregon
best stay
Eisha Moreno
Vancouver, WA
best stay
November 17, 2015
I was very impressed with the cleanliness of the rental and how beautiful the updates were. The amenities were very convenient. The view was beyond amazing and I look forward to future stays here. I recommend this unit to all, you will love it.
Excellent Value
Amanda Sullivan
Kuna ID
Excellent Value
August 17, 2013
Excellent value! Great location, clean and stocked well.
Leak in the guest bathroom
Heather Bussmann
Bend, OR USA
Leak in the guest bathroom
August 2, 2013
Great view, access to, and location on the beach. The first night the neighbors in the unit above us took a bath and when they let the water drain, a lot of it drained right into our bathroom through the heat lamp. We called the owner, so hopefully it is fixed before the next renters settle in. We were not compensated in any way and a little dangerous. The rest of the trip was excellent and the neighbors said they would not use that bathroom again, so our bathroom stayed dry.
Owner's reply:
Thank you for your review. The issue you experienced was directly related to a maintenence need in the owners unit above mine and has been rectified. I did come by the unit to speak with you directly the next morning but you were not there.I followed up Monday evening with a phone call and left a message asking you to please call me in hopes of rectifying the matter as best I could given the situation was outside my control. Having not heard from you, please accept my apologies.
Cozy Comfy Condo :)
Lynne Richardson Roy
Redmond, OR
Cozy Comfy Condo :)
May 30, 2013
We enjoyed our stay in this clean and tasteful little condo. It was a bit tight with 5 sleeping, but as a family that worked just fine for us (We had the kids sleep in the bedroom so hubby and I could have our morning coffee open to the ocean sights/sounds without disturbing the kids). The coded door lock is so much easier than trying to keep track of where keys/cards are. Kids loved the pool, and even though we were there in cool weather that would be a bonus even in summer since our northern oceans are not exactly warm! Thank you for sharing your condo and being willing to rent for 2 nights on a holiday weekend at a reasonable price.
Torie Upright
April 10, 2013
This condo is very cozy, well decorated, and has all the amenities of home. My kids loved the pool and the fact that it is on the beach. We will definitely stay again and recommend it to our friends and family. Thank you for sharing.
A Last Minute Booking
Thomas Lunsford
Portland, OR
A Last Minute Booking
March 22, 2013
My mom and nephew decided to pay me a vista (from Hawaii) last December, and wanted to experience the Oregon coast. Uncertain on where to take them, I decided to book a last minute stay at this condo. Adrianne was very accommodating and made sure everything was setup before we arrived. We enjoyed how specious and homely the condo felt. My mom and nephew slept comfortably in the bedroom, while I enjoyed sleeping on the foldout couch in the living-room (I love waking up to the sounds of the ocean). It was nice having separate bathrooms. The condo was clean, comfortable, and well decorated. Needless to say, we enjoyed our two day trip to Lincoln City. I highly recommend this condo to anyone looking for a comfortable and relaxing stay. I know I'll be going back!
Beautiful ocean view
Luann Horn
Hermiston, Or
Beautiful ocean view
September 6, 2012
The condo was very nice and clean. Tastefully decorated. Well stocked kitchen, bath towels. etc..The only extra's we could have used was garbage bags and tp. Now for that view and easy access to the beach. Wow couldn't have asked for better! Having a pool and sauna was a nice bonus.
Awesome vacation rental
Minh Ly
Tigard, OR
Awesome vacation rental
August 23, 2012
This condo is by far the best looking one with updated lighting and furniture. I loved how close it was to the beach and down the street from the shopping area. Make sure you leave the sliding door open when you're there so you can hear the ocean. It's amazing! Condo person also provides chairs and blankets to go out on the beach. Would love to see a small washer/dryer the next time we come back..
Puffer Fish
Shirey Matz
Battle ground, Wa.
Puffer Fish
July 11, 2012
We loved it. The only complaint would be the bedroom light didn't work and neither did the bedroom t.v. remote. Oh and we needed more garbage bags. But the condo and the closeness to the beach were awesome.
Owner's reply:
The lights in the bedroom are functional but are operated by two separate control switches which can cause some confusion and requires the bedside lamps to be in the on position for the switch to work. The remote has been replaced, thank you for letting me know. Overall glad you enjoyed your stay.
Carol Hagler
Vancouver, WA USA
June 29, 2012
We found the condo very accomadationg, plenty of room for everyone. The only thing missing was a dishwasher. Had a great view of the kite festfull.
Owner's reply:
Thank you for taking the time to share your feedback so glad to know you enjoyed your stay. We did look into the option of adding a dishwasher to the unit however, the condo HOA at this time is not issuing approvals for upgrades of that type to the units. If in the future the approval is granted we would again consider that addition.
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Radamanthis Hotel Crete
Adelianos Campos, Crete 74100, Greece
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Radamanthis Hotel Crete Hotel Details
Radamanthys apartments are a small and friendly complex located in Sfakaki area of Rethymno. The complex is ideal for couples as there is weekly disco and barbecue entertainment.
Adelianos Campos Crete 74100, Greece

subset: commoncrawl


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February 22 to 23, 2017
Type: Music
An evening reuniting three popular French-Canadian singers revisiting the Big Band music of the swing era....
February 22, 2017
Type: Music
A bilingual singer/songwriter from Quebec, Kevin Parent spent the majority of his career as a Francophone artist....
October 14, 2016 • December 16, 2016 • February 22, 2017
Type: Sports & Outdoors
Québec City's major junior hockey team for players 16 to 20 years old. The Quebec Remparts are part of the Quebec Major Junior Hockey League (QMJHL)....
February 22 to 26, 2017
Type: Trade Shows & Fairs
A fair dedicated to housing and the art of living featuring more than 300 exhibitors....
Major Event
June 18, 2016 to September 4, 2017
Type: Museums and Exhibitions
What do we really know about our four-footed friends? The exhibition Like Cats and Dogs introduces visitors aged 6 and over to a wealth of scientific, sociological, and cultural findings about these ...
Major Event
February 17 to May 14, 2017
Type: Museums and Exhibitions
One of Canada's foremost contemporary art biennials, the Manif d'art – The Quebec City Biennial presents the work of some 100 artists from all disciplines...
October 20, 2016 to February 26, 2017
Type: Museums and Exhibitions
Fighting in Flanders – Gas. Mud. Memory. shares the experiences of Canadian soldiers and Belgian citizens through wartime artifacts...
February 2 to 26, 2017
Type: Museums and Exhibitions
A wonderful opportunity for those interested in learning more about the new forms of multidisciplinary and electronic art. Meet artists from here and abroad. The only festival of its kind in Québec C...
June 1, 2016 to March 10, 2017
Type: Museums and Exhibitions
Discover 25 events that, since the fall of the Berlin Wall, have left a mark on our time. Through 18 filmed interviews with key actors in these events, filmmaker Hugo Latulippe traces the roots of th...
March 8, 2016 to April 2, 2017
Type: Museums and Exhibitions
Today's "nanos" can be found in electronic gadgets, cosmetics, sports equipment, and medical treatments. Thousands more promising new applications are also on the way. Nanotechnology has drawn o...
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This CCTV BNC extender can be used with any DVR or CCTV set up to extend the BNC connection. This extenders works 1:1. It comes as a set transmitter and receiver.
Copyright © 2017 HDCabling - Centurion / Boksburg, HDMI Splitters, HDMI Cables, CAT6e UTP, CAT7 STP, CAT5e FTP, DSTV & Multichoice Equipment, Optical Toslink, Switches, Apple Ipad Accessories, VGA Splitters, RCA Switches, Multiswitches, mini-Displayport, FirewireCopyright 2014 HDCabling CC


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Girls Articles
Girls TV Series recaps, updates, analysis and photos.
Sasha's INTO IT: Poler "The Napsack"
November 30, 2012 19:10:43 Posted at November 30, 2012 19:10:43
WHAT: POLER "THE NAPSACK" WHERE: CLICK HERE WHY: I know what you're thinking. This is just another thing to further feed my insane craving for comfort. And you're right. It is. Full Article


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Ragi Rotis
|Ragi flour
|2 Cup (32 tbs)
|Rice flour
|1⁄2 Cup (8 tbs)
|Grated coconut
|1 Cup (16 tbs)
|Chopped onion
|1⁄2 Cup (8 tbs)
|1 Tablespoon, chopped
|Green chilies
|3 , chopped
|To Taste
|1 Tablespoon
Mix all the ingredients in a large bowl.
Knead to form a thick dough.
Add water only if, required.
Divide the dough into small balls.
Grease some kitchen paper with a few drops of oil.
Place the ball in the center of the paper and with your fingers moistened with water pat the dough to flatten it.
Pat the ball into as thin a roti as possible without breaking it.
Moisten your fingers as often as you wish without thinning the dough too much.
In the beginning your ragi rotis will turn out a little thick, but with practice they 11 start getting thinner.Believe me, this dish is well worth the effort. And you'll pick up speed as you go along.
Heat a griddle and place the roti on this with the paper facing you.
Peel off the paper gently once the side on the griddle begins to bake.
Drizzle a few drops of oil around the roti.
Bake on both sides.
Meanwhile grease another piece of kitchen paper and repeat the process.
Using 2 kitchen papers repeat the process till all the dough is used up.
Serve hot with a dab of homemade butter or plain yogurt and a thin lentil soup.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 1801 Calories from Fat 474
% Daily Value*
Total Fat 53 g81.1%
Saturated Fat 26 g130.2%
Trans Fat 0 g
Cholesterol 0 mg
Sodium 435.1 mg18.1%
Total Carbohydrates 296 g98.6%
Dietary Fiber 49.9 g199.5%
Sugars 10.1 g
Protein 38 g75.8%
Vitamin A 27.3% Vitamin C 142.3%
Calcium 5.3% Iron 16.7%
*Based on a 2000 Calorie diet


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Black Witches in Wichita, Kansas
Merry Meet and Blessed Be! My name is Phelan, I am a white witch that practices white witchcraft, I am also a gifted psychic and root worker. I strive to provide ethical and honest services to all of our clients in Wichita, Kansas or Barcelona, Spain from our Atlanta location. If you are in need of assistance with prosperity spells that work, simple rituals, magic spells for prosperity or luck spells charms, call us at 678-918-3333 or create an account today, if you live within the Atlanta area we can setup an appointment in person to discuss your situation or to schedule a psychic reading.
ContactPhelan.com has an ethical commitment to provide our clients with the best service in Wichita, Kansas from our Atlanta location. If you are thinking about our "Black Witches" in Wichita, Kansas or " Black-magic " in Barcelona, Spain create an account and I will be more than happy to discuss your situation or any other questions that you may have.
Jealous foes will often launch a psychic or magical attack against the one they envy, do you feel that you are under attack? If so contact me today to get the esoteric help that you need to shield yourself and guard your relationship from unwanted advances!
May your journey be safe and pleasant, may your lessons however hard they may be, be learned well. Carry with you in mind and in heart love and compassion of self, self-respect and a desire to learn and to grow spiritually. May the Mother bless you with light, love, abundance and prosperity!
Blessed Be, Phelan
Black Witches | Lottery Spells | Wichita, Kansas


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(Ventilation Accessories - Filters)
Charcoal filters are available for use with range hoods that re-circulate filtered air back into the kitchen. These filters are designed to absorb odors as well as filter out contaminants.Ask an Expert
|These filters are designed to absorb odors as well as filter out contaminants.
|Net Weight
|1.5 lb(s) / 0.68 kg
|1 year(s)
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Black And White Nut Brownies
|1 Cup (16 tbs)
|Vanilla extract
|1 1⁄2 Teaspoon
|2 Cup (32 tbs)
|Sifted all purpose flour
|2 Cup (32 tbs)
|1⁄2 Teaspoon
|Chopped nuts
|2 Cup (32 tbs)
|Unsweetened chocolate square
|4 Ounce (2 Square, 2 Ounce Each)
|Chocolate frosting
|1 Cup (16 tbs)
Cream butter until light and fluffy.
Beat in vanilla and sugar.
Add eggs, one at a time, beating well after each addition.
Add flour and salt and mix until blended.
Stir in nuts; divide batter into halves; add cooled melted chocolate to one.
Drop batters alternately by teaspoons onto greased, floured pan (13 x 9 x 2 inches).
Run knife through batter several times to marbleize.
Bake in preheated moderate oven (350°F.) for about 45 minutes.
Turn out on rack to cool.
At serving time, cut into bars about 3 x 1 1/2 inches.
Makes 24 bars.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size
Calories 669 Calories from Fat 355
% Daily Value*
Total Fat 40 g61.2%
Saturated Fat 16.9 g84.7%
Trans Fat 0 g
Cholesterol 110.8 mg36.9%
Sodium 245.8 mg10.2%
Total Carbohydrates 74 g24.8%
Dietary Fiber 4.1 g16.4%
Sugars 50.9 g
Protein 11 g22.1%
Vitamin A 11% Vitamin C
Calcium 6.3% Iron 18.5%
*Based on a 2000 Calorie diet


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Horses Spot the Difference
by: M2H • 360
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by: M2H • 360
A cute and addictive spot the difference game with a horses theme. Spot all differences in random pictures and beat the highscore.
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Oriental Stir Fried Shark Salad
|Salad oil
|3 Tablespoon
|Boneless skinless shark/Halibut
|1 1⁄4 Pound (1 Inch Thick)
|Minced ginger
|1 Tablespoon
|Celery slices
|2 Cup (32 tbs)
|Edible pea pods
|1⁄2 Pound (Ends And Strings Removed)
|Ramen noodles
|3 Ounce (1 Package Omit Seasoning Packet)
|Oriental sesame oil
|2 Tablespoon
|Seasoned rice vinegar
|1⁄2 Cup (8 tbs)
|Cilantro sprigs
|1⁄2 Cup (8 tbs) (Coriander)
Place 2 tablespoons salad oil in a wok or 10 to 12-inch frying pan over medium-high heat.
Add fish and ginger; stir-fry until fish is slightly translucent but still moist in center (cut to test), 4 to 6 minutes.
Lift from wok; set aside.
Add remaining salad oil, celery, and peas to wok.
Stir-fry until vegetables are tender-crisp to bite, 4 to 5 minutes.
Remove from heat.
Gently stir in fish, noodles (break into bite-size pieces), sesame oil, soy, vinegar, and cilantro.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 2065 Calories from Fat 870
% Daily Value*
Total Fat 98 g150.9%
Saturated Fat 13.1 g65.3%
Trans Fat 0.8 g
Cholesterol 300 mg100%
Sodium 3425.3 mg142.7%
Total Carbohydrates 126 g42.1%
Dietary Fiber 12.3 g49.3%
Sugars 56.7 g
Protein 141 g282.7%
Vitamin A 82.4% Vitamin C 244.3%
Calcium 21.5% Iron 50.4%
*Based on a 2000 Calorie diet


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Maiku Kamishiro has always had a photo of himself as a child in front of an old house with a young girl. One day after spotting the building in a news report, the high school boy moves to the countryside to live in the house from the picture. While living alone, making ends meet by working as a freelance programmer, Maiku gets the shock of his life when not one, but two girls turn up on his doorstep claiming to be his twin sister! With the lively Miina and timid Karen both possessing the same photograph, the pair begins living with the confused Maiku as they try to figure out which one of them is actually his sister.
5 votes
5 votes
5 votes
5 votes
Please Twins, if nothing else, is a prime example of missed potential. Here we have a truly brilliant premise that is pretty much completely ruined by its follow-up story and ending. So much more could have been added to the following episodes to keep the story interesting, but no: the plot instead sinks into autopilot, and the result is horrifically boring and... read more
Please Twins. This anime is definately going to become on of my favorites (some parts made me giddy!). The story deserves between an 8 and a 9 due to the fact that it is a short series that is intended to sell manga books, I just like shows that last a bit longer. The animation is rather good keeping in mind that this anime is from 2003, and this quality would have passed for exceptional back then. Keep the... read more
Please Teacher
Please Teacher: Secret Couple
Please Teacher: Marie Love Theater
Please Teacher: Reminiscence
Please Twins: The Summer Never Ends
|Side Story
Onegai Teacher
Onegai Teacher (Light Novel)
Onegai Twins (Light Novel)
Onegai Twins
|Original Manga
Character Design


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Melody, I'm not much of a recipe person. I usually just cook what seems to
go together well, and it comes out tasting good. I do enjoy reading
cookbooks, though, for ideas, and I have a pretty large collection of them.
Well, I started looking for a recipe for Eggplant Parmigiana, thinking that
would be a good starting place for someone who hasn't eaten eggplant. Some
time and about 15 cookbooks later, I finally found one, but unfortunately it
was not very explicit. What I did determine, however, was that basic
eggplant preparation usually starts with frying or sauteeing slices or cubes
of eggplant in a small amount of olive oil until softened. Then layer the
eggplant slices in a baking dish with your best tomato sauce and mozzarella
cheese, top with parmesan cheese and bake until bubbly and brown. There are
all sorts of yummy variations using various meats. The tomato sauce should
be rich with bell peppers, onions, garlic and red wine. Olives are
frequently added. This is a good way to use left-over leg of lamb.
Of course, just fried eggplant, either dipped in egg and cracker crumbs, or
seasoned flour, ranks right up there with fried okra and fried green
tomatoes, but I can't in good conscience recommend it, since fried foods are
now so politically incorrect. I do hope you'll try eggplant, however. It is
really one of the good things, and the fruits are so pretty - either the
purple or the white kinds. Auralie
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text:
Ginsenoside Rh1 potentiates dexamethasone's anti-inflammatory effects for chronic inflammatory disease by reversing dexamethasone-induced resistance
- Jun Li†1,
- Juan Du†1,
- Dong Liu1,
- Binbin Cheng1,
- Fanfu Fang1,
- Li Weng1,
- Chen Wang1Email author and
- Changquan Ling1Email author
DOI: 10.1186/ar4556
© Li et al.; licensee BioMed Central Ltd. 2014
Received: 31 August 2013
Accepted: 15 April 2014
Published: 1 May 2014
Acquired resistance to glucocorticoids constitutes a major clinical challenge, often overlooked in the search for compounds to improve the effect of classic steroids. We sought to unravel how a plant-original compound, ginsenoside Rh1, potentiates dexmethasone (DEX)'s potential anti-inflammation properties.
Ginsenoside Rh1 combined with DEX was applied in a short-term and long-term treatment protocol for inflammation. Its potential mechanism on anti-inflammation was explored. In addition, the effect of Rh1 on the side-effect induced by DEX was studied. Furthermore, the in vivo anti-inflammatory effects of Rh1 combined with DEX were evaluated in a collagen-induced arthritis (CIA) mice model.
Ginsenoside Rh1 potentiates DEX's anti-inflammatory effects even after prolonged DEX treatment. Rh1 could improve the glucocorticoid receptor (GR)'s transrepression on nuclear factor kappa B (NF-κB) and transactivation on dual specificity protein phosphatase 1 (DUSP1), which is responsible for DEX's anti-inflammatory effects. Parallel Western blot assay and radioligand binding analysis revealed that Rh1 could increase the expression and binding of GR. This is in sharp contrast to DEX alone, showing a direct link among prolonged treatment, decreasing GR and the abolishment of anti-inflammation. Interestingly, Rh1 does not enhance the transactivation of glucocorticoid-responsive elements (GRE) driven genes - gluconeogenic enzyme glucose-6-phosphatase (G6P) and phosphoenolpyruvate carboxykinasee phosphatase (PEPCK) in primary mouse hepatocytes, a mechanism partly held accountable for the metabolic side-effects. Similar results were found in CIA mice.
Rh1 could potentiate DEX's anti-inflammatory effects and does not cause a hyperglycemic side effect. Ginsenoside Rh1 combined with DEX may be a promising candidate treatment option for chronic inflammatory diseases in need of long-term immunosuppression therapies.
Glucocorticoids (GCs) are still the cornerstone drugs used in treatment protocols of a wide range of inflammatory and immune disorders. However, long-term and/or high-dose GC administration is commonly associated with adverse side effects, such as hyperglycemia, weight gain, osteoporosis, depression and decreased immunological function. Furthermore, patients on glucocorticoids can develop reduced glucocorticoid sensitivity and even resistance. It has been reported that approximately 30% of patients failed to respond to even high doses of glucocorticoids [1, 2].
Different molecular mechanisms have been responsible for the phenomenon of acquired glucocorticoid resistance, including reduced expression of the glucocorticoid receptor (GR), altered affinity of GR for the ligand, reduced ability of GR to bind DNA or increased expression of inflammatory transcription factors, such as AP-1, that compete for DNA binding [3–5].
Current research is focused on finding compounds with similar anti-inflammatory potency of the standard GCs but with reduced side effects [6–9]. Nevertheless, it is currently unclear whether simply dissociating activation from repression of GR in a ligand will result in a beneficial therapeutic profile. Actually, the powerful anti-inflammatory effect of GCs is complex, and likely due to both repression of a large number of pro-inflammatory cytokines and mediators, as well as activation of anti-inflammatory genes, such as IL-10, IL-4, TGF-β, DUSP1 and Annexin A1 [10–12]. As a result, novel compounds that lack a capacity for steroid-inducible genes may in fact show lessened anti-inflammatory effects [13–15]. In fact, the regulation of GR may be another efficient strategy for restoring glucocorticoid resistance.
Over the past years, our team has had a continued interest in the regulation of GR with Chinese medicinal herbs [16, 17]. We have demonstrated that the main extracts of ginseng, ginsenosides (GSS), which is one of the derivatives of triterpenoid dammarane consisting of 30 carbon atoms, could partially reverse dexmethasone (DEX)-induced down-regulation of GR in vitro and in vivo [18]. But there are many ingredients in GSS. Ginsenoside Rh1 is one of the major one of these.
We thus studied the effect of ginsenoside Rh1 on GR and how this compound improved DEX's anti-inflammatory potential by regulating GR after prolonged DEX treatment. In addition, we unraveled the potential of Rh1 on GCs-induced hyperglycemia. Also, we evaluated the anti-inflammatory effects of Rh1 combined with DEX in vivo.
Materials and methods
Cytokines and reagents
Recombinant murine TNF-α was purchased from R&D. Ginsenoside Rh1 was supplied by Shanghai Dongfang Pharmaceutical (Shanghai, China). Rabbit polyclonal Ab to GR was obtained from Abcam (Cambridge, MA, USA). Phospho-IκBa (Ser32) rabbit mAb, phospho-NF-κB p65 (Ser536), phospho-p38, IκBa (44D4) rabbit mAb, NF-κB p65 and p38 Abs were supplied by Cell Signaling Technology (Danvers, MA, USA).
Cell culture
The murine macrophage RAW264.7 cells were purchased from American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin in a 5% CO2 humidified incubator at 37°C. The primary mouse hepatocytes were isolated and cultured as described in [19].
Western blot analysis
Cells were seeded in six-well plates. Total protein, cytoplasmic and nuclear proteins were extracted as described previously [20].
Real-time PCR
RT-PCR was performed as described in [21]. Primer sets for interleukin-6 (IL-6), IL-17, matrix matalloproteinase-1 (MMP-1), tumor necrosis factor-alpha (TNF-α), phosphoenolpyruvate carboxykinasee phosphatase (PEPCK), gluconeogenic enzyme glucose-6-phosphatase (G6P), dual specificity protein phosphatase 1 (DUSP-1), GR and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are available upon request.
IL-6 and IL-17 levels were determined by ELISA kits according to the manufacturer's instructions (Bender MedSystems, Vienna, Austria).
Radioligand binding analysis for GR
Radioligand binding analysis was performed as described in [18].
Transient transfection and luciferase assay
After RAW264.7 cells reached 90 to 95% confluence in 24-well dishes, they were transfected using Lipofectamine 2000 (Invitrogen) with 0.6 mg/ml p-GR-Luc and 0.08 mg/ml p-Renilla luciferase-thymidine kinase for normalization in serum-free medium. Four hours later, the medium was replaced with DMEM containing 10% FBS. Twenty-four hours post-transfection, cells were pretreated for 1 h with or without 10 μM Rh1, after which DEX (1 μM) was added for 24 h. Then the reporter gene activity was measured according to the recommendation of the manufacturer (Promega, Madison, WI, USA). Results were normalized by thymidine kinase reporter activity.
Collagen-induced arthritis mouse model
Male DBA/1 mice (six to eight weeks old) were purchased from the Second Military Medical University Laboratory Animal Center (Shanghai, China). The induction of collagen-induced arthritis (CIA) in DBA/1 mice is described in [22, 23]. Once arthritis was evident, the animals were randomized in one of the following treatment groups with eight mice per group: vehicle, Rh1 and DEX, and DEX by intraperitoneal (i.p.) therapy for 10 d. An additional eight normal mice were as blank group. The clinical severity of arthritic paws was graded according to standard evaluation procedures. For more details refer to [22].
All procedures involving animals were performed in accordance with the European Communities Council Directive of November 24, 1986 (86/609 EEC) and approved by the Ethics Committee of Changhai Hospital.
Blood glucose determination
Food was removed overnight. Blood samples were taken by caudal vein puncture under ethyl ether anesthesia. Blood glucose levels were determined by CareSens blood glucose monitoring system (i-SENS, Seoul, Korea) according to the manufacturer's instructions.
Histological evaluation
Knees were dissected post-mortem, fixed in 10% formalized saline, decalcified, dehydrated and embedded in paraffin. Sections of 5 μm were made and stained with hematoxylin-eosin staining. Serial sections were evaluated by two blinded investigators. The inflammatory metacarpophalangeal joints were analyzed according to different parameters, including the influx of inflammatory cells in joint capsules, cartilage destruction, joint cavity narrowing, influx of inflammatory cells in marrow cavities and periosteal thickening.
All procedures involving animals were performed in accordance with the European Communities Council Directive of November 24, 1986 (86/609 EEC) and approved by the Ethics Committee of Changhai Hospital.
Statistical analysis
All data were presented as the means ± standard deviation (S.D). Statistical significance was determined using SPSS 11.0 for Windows from SPSS Inc. Chicago, Illinois, USA. Data analysis was performed by one-way analysis of variance (ANOVA), followed by Fisher's least significant difference (LSD). Differences with P- values < 0.05 were considered to be statistically significant.
Ginsenoside Rh1 combined with DEX inhibited the expression of pro-inflammatory cytokines, even after long-time treatment
Our recent research showed that ginsenoside Rh1 could reverse the reduction of GR expression and binding capacity induced by DEX (Additional file 1: Figure S1). And in the presence of 10 μM Rh1, the reversal effect was the most significant.
In order to observe if Rh1 could improve the anti-inflammatory potential of DEX, we compared the therapeutic potential of both treatment protocols (Rh1 combined with DEX and DEX treatment alone) in two different experimental settings. First, we pretreated the RAW264.7 cells with solvent, DEX (1 μM) or DEX combined with Rh1 (10 μM, 1 μM, 0.1 μM) or Rh1 alone for 2 h, after which TNF-α (20 ng/ml) was added for 8 h. The anti-inflammatory agents thus resided on the cells for a time span of 10 h, hereby mimicking a short-term treatment protocol. Alternatively, we pretreated the cells with solvent, DEX (1 μM) or DEX combined with Rh1 (10 μM, 1 μM, 0.1 μM ) or Rh1 alone for 24 h, after which TNF-α was added for 8 h. The anti-inflammatory agents were thus kept on the cells for 32 h, reflecting a prolonged treatment protocol.
Ginsenoside Rh1 increased DEX induced inactivation of NF-κB and activation of DUSP1
Another mechanism by which glucocorticoids inhibit inflammation is through induction of DUSP1. Figure 2E showed that either in short-term treatment or prolonged treatment protocol, DEX could increase the transcription of DUSP1 with or without TNF-α. It was worth noting that the degree of activation of DUSP1 by DEX in 24 h-treatment group is lower than those in the 2 h-treatment group. More important, Rh1 could enhance DEX-induced DUSP1 expression in both protocols. However, Rh1 alone had no effect on the transcription of DUSP1.
Ginsenoside Rh1 potentiated DEX induced inactivation of p38
Improvement of GR was responsible for ginsenoside Rh1 enhancing the anti-inflammatory potential of DEX after long-time treatment
Ginsenoside Rh1 exhibited a little promotion on GRE promotor activity but played different roles on GRE-driven genes expression
Ginsenoside Rh1 combined with DEX markedly attenuated inflammation in CIA model
After clinical assessment for 10 days, mice were sacrificed and paw joints were histologically examined to determine whether the amelioration of clinical disease activity was accompanied with reduced histopathological signs of inflammation and tissue damage. The majority of joints from vehicle-treated animals showed evidence of the influx of inflammatory cells in marrow cavities and joint capsules, destruction of articular cartilage and inflammation resulting in periosteal thickening (Figure 6B-b). In this analysis, only 30% of the joints from the DEX-treated mice were found to be normal in all assessed parameters, which still showed inflammatory cells in marrow cavities and joint capsules, and periosteum thickening (Figure 6B-c). However, Rh1 combined with DEX treatment group was accompanied by normal histopathologic features in all animals. The effects were most apparent with regard to the reduction in the degree of inflammation in the joint capsules and periosteum (Figure 6B-d).
A parallel Real-time PCR for GR mRNA was performed. GR mRNA after DEX treatment was significantly lower than those after Rh1 combined with DEX treatment (P <0.01), and this was reflected by its efficiency in anti-inflammation in CIA as described in vitro (Figure 6C).
Additionally, effects of Rh1 on blood glucose were also observed in vivo. High blood glucose levels were found in the DEX treatment group (P <0.01) but not in the Rh1 combined with DEX group (P >0.05, Figure 6D). Furthermore, by RT-PCR analysis, Rh1 could ameliorate DEX-induced high expression of the PEPCK or the G-6-P in CIA (P <0.05, Figure 6E). It seemed that Rh1 combined with DEX had fewer negative effects than DEX treatment alone on blood glucose in vivo.
The present study demonstrates some intriguing molecular aspects of glucocorticoid receptor biology, which have to be kept in mind when developing new strategies to reduce glucocorticoid resistance and related side effects. The results showed that ginsenoside Rh1 could improve the anti-inflammatory potential of DEX even after prolonged treatment. Most interestingly, Rh1 could ameliorate DEX- induced hyperglycemia in vitro and in vivo.
The mechanisms linked to the phenomenon of glucocorticoid resistance are complex [4, 24]. Several therapeutic strategies for the management of glucocorticoid-resistant diseases have been proposed, but most important approaches involve improving anti-inflammatory potential of glucocorticoids to reduce treatment time or reversing the molecular mechanisms of glucocorticoid resistance. Some selective glucocorticoid receptor agonists (SEGRAs; or dissociated steroids) may exert their anti-inflammation without provoking ligand-induced resistance [9]. However, it is unlikely that SEGRAs will be able to overcome glucocorticoid resistance because they might be as ineffective as conventional glucocorticoids in triggering gene activation or repression [25]. Alternative anti-inflammatory drugs are currently available for the treatment of glucocorticoid-resistant diseases, but these drugs are likely to have issues with toxicity and side-effects, or are only suitable for topical application [26].
The results presented in this study showed that RAW 264.7 cells were only initially responsive to the therapeutic effects of DEX after a short-term treatment, but for a prolonged time, the cells became insensitive to DEX. When the cells were treated with DEX combined with Rh1, the anti-inflammatory potential of DEX was preserved in both short and prolonged usage. Rh1 itself had no effects on TNF-induced cytokines, so the heightened anti-inflammatory effect of Rh1 combined with DEX may be attributed to Rh1's improvement on GC-GR interaction. In fact, GCs exhibit potent anti-inflammatory effects through two main mechanisms. First, they inhibit the transcription of pro-inflammatory genes via suppression of the transcriptional activation induced by AP-1 and NFκB. Second, they induce genes that antagonize the inflammatory response, including the glucocorticoid-induced leucine zipper (GILZ) and DUSP1 [26]. Our data showed that in contrast to DEX, Rh1 combined with DEX still could attenuate p65 cytoplasmic-to-nuclear translocation after prolonged treatment, which is a prerequisite for activation of NFκB, by decreasing the phosphorylation of IκBα and attenuating IκBα degradation. Also, Rh1 combined with DEX induced a higher expression of DUSP1 than DEX alone in either short-term treatment or prolonged treatment. DUSP1 is a member of a large family of multifunctional phosphatases that reside in the nucleus and specifically dephosphorylate and inactivate members of the MAPK family, such as JNK, p38 MAPK and ERK [4, 24]. Increased expression of DUSP1 attenuates p38 MAPK signaling, which disrupts the signal leading to the induction of pro-inflammatory gene expression. In this report, we demonstrated that dephosphorylation of p38 declined after prolonged treatment with DEX. But Rh1 combined with DEX resulted in striking inhibition of p38 activation even after prolonged usage, which is accordant to the DUSP1 result. These findings together show that Rh1 can reinforce the anti-inflammatory potential of DEX and alleviate GC resistance induced by prolonged DEX treatment. More importantly, this improvement was a result of both transrepression and transactivation of GR. Since down-regulation of GR is associated with the loss of GCs' responsiveness, this aspect was also examined in our study. The results indicated that Rh1 could ameliorate DEX-induced down-regulation of GR expression and binding, in accordance with the retained anti-inflammatory effects even after prolonged treatment. This is in contrast to DEX alone, showing a direct link between prolonged treatment, decreasing GR and the abolishment of the anti-inflammatory effects. Furthermore, as Rh1-induced up-regulation of GR was blocked by the co-treatment with actinomycin-D or cycloheximide, de novo mRNA transcription and protein synthesis are most likely involved. These findings suggested that long-term Rh1 treatment might not only increase the transcription of GR, but also its synthesis in RAW macrophages. And Rh1's up-regulated expression of GR and dephosphorylation of p38MAPK might be responsible for its improvement on GR ligand binding. It was reported that activated p38 targeted the GR ligand-binding domain indirectly to suppress GR function [27]. Therefore, inhibition of p38 phosphorylation by Rh1 may benefit GR ligand binding. The role of p38 in the mechanism of action of Rh1 on GR ligand binding is therefore worthy of further study.
Although Rh1 could increase DEX-induced GRE activation and DUSP1 expression, Rh1 combined with DEX did not induce high expression of G-6-P and PEPCK in primary hepatocytes from mice, which is important in gluconeogenesis. It was interesting that Rh1 played different roles on GRE-driven genes. Therefore, the structural changes of GR after Rh1 treatment, as well as some transcriptional factors involved require further study. Altogether, because the most attractive option for treating glucocorticoid resistance is to directly address the cause, Rh1 combined with DEX may be an effective treatment protocol for acquired glucocorticoid resistance with down-regulation of GR protein levels and binding.
To confirm our in vitro data, we examined the role of Rh1 combined with DEX in the CIA model. It seems that Rh1 is able to improve the anti-inflammatory effects of DEX in vivo. In line with this, improved GR expression was found in liver isolated from mice treated with Rh1 combined with DEX. Meanwhile, DEX combined with Rh1 had no unwanted effects on blood glucose levels, in sharp contrast with the effect of DEX treatment alone. Further analysis of G6P and PEPCK GRE-driven gene expression in the liver demonstrated the beneficial regulatory effect of Rh1 on glucose metabolism. Although the CIA model did not mimic GC-resistance in vivo, our data still supported that Rh1 increased the anti-inflammatory potential of DEX without hyperglycemia and improved DEX-induced down-regulation of GR in vivo.
Ginsenoside Rh1 potentiated DEX's anti-inflammatory effects by improving DEX-induced down-regulation of GR. Our data also showed that ginsenoside Rh1 combined with DEX did not cause hyperglycemia. As a result, ginsenoside Rh1 combined with DEX may be a promising candidate treatment option for chronic inflammatory diseases that require long-term immunosuppression therapies and so is worthy of study.
Authors' information
Jun Li and Juan Du are listed equally as first authors.
Collagen-induced arthritis
Dulbecco's modified Eagle's medium
dual specificity protein phosphatase 1
fetal bovine serum
gluconeogenic enzyme glucose-6-phosphatase
glyceraldehyde 3-phosphate dehydrogenase
- GCs:
glucocorticoid-induced leucine zipper
glucocorticoid receptor
glucocorticoid-responsive elements
matrix matalloproteinase-1
- NF-κB:
nuclear factor kappa B
phosphate-buffered saline
phosphoenolpyruvate carboxykinasee phosphatase
- SEGRAs:
selective glucocorticoid receptor agonists
- TNF-α:
tumor necrosis factor-alpha.
This work was supported by the National Nature Science Foundation of China (grant numbers: 81001672 and 30810103910). The discussion was revised by Mr. Yu Xu from Johns Hopkins University and Mr. Pok Fai Wong from the University of Sydney.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Distributed on Fridays via e-mail to all Medical School employees, students, residents, and postdoctoral fellows, UT 2 Me is Dean Giuseppe Colasurdo's weekly update of news and items of interest. He also welcomes feedback through this two-way communication.
I am so pleased to tell you about the much improved performance of the UT group practice for the first quarter of this fiscal year. We are up $7 million in charges and approximately $1.5 million ahead in collections. This is great news for UT Physicians and the Medical School, since you may know that during the last half of the year we are usually trying to make up ground for the first half.
Some of you may not know why UT Physicians is so important to the Medical School. These dozens of adult and pediatric clinics, the majority of which are located across the street from the Medical School in the UT Professional Building, are where our clinical faculty see patients, and where our residents, fellows, and students learn the fine points of patient care. The income from these clinics goes back to the Medical School in operating support. With decreased funding from the state Legislature and increased competition for national grants, we rely upon our income from our UT Physician clinics.
Patient visits to our UT Physician clinics have been flat at approximately 170,000 over the last six years. With the leadership from UT Physicians and the clinical chairs, we will be looking at why our growth has been stunted and how we can solve this problem. One area we will be working on immediately is to readjust our booking schedule, taking the no-show rate into consideration – in the first two months of our current fiscal 53,000 visits were scheduled, yet only 32,000 patients were seen. If we can schedule patients based upon the historic no-show rate (variable among clinics), we should be able to see more patients, boost morale of the patients – who will be able to have a shorter wait to see a specialist, and experience growth. Every clinical encounter counts in the Medical School MSRDP revenue - $210 million or 60% of the total revenue in FY07.
The bottom line is that these are our UT clinics, and we determine what type of experience we want to offer to the community – it can be an exceptional experience for the patient, or it can be less than stellar. Medical School and UT Physicians administration is willing to help, but it boils down to everyone being a great citizen by providing an exceptional patient care experience built on quality and service.
It is in this spirit of excellent patient care that we must remember to be available to our patients during the holidays. The Medical School may be on break, but our level of care must remain high. The hospitals are full and there are long waits in the emergency room now, as the seasonal rush begins. With the increase in volume and activity, faculty must help each other to deal with the patient loads. The addition of inpatient beds to open at Memorial Hermann – Texas Medical Center in the new year will help with this demand and allow us to continue our growth of clinical services.
At the chairs meeting this week, I was encouraged to hear that when it comes to faculty retention, only two chairs cite compensation as the problem. We want to retain all of our good employees, faculty and staff, and I know that it takes good leadership – someone who is sensitive to needs and who promotes career development – to retain good people. I depend upon the chairs and chiefs to implement their department's vision and encourage them to ask for help when it comes to career development and the retention of critical faculty. This is a challenging task in academic medicine these days due to limited state funding for non-RVU generating activities. However, our school is committed to explore new initiatives that will promote faculty retention and academic growth as we continue to enrich the overall teaching experience of our students and residents.
Speaking of critical faculty, I am happy to announce that Dr. Lenaine Westney will be our permanent Chief of the Division of Urology. She has done a solid job in the interim role over the last two-and-a-half years, and I know that she will continue to excel. Dr. Westney will continue to grow her Division and will expand her collaboration with MDA Cancer Center and Dr. Kevin Slawin at MHH.
In other good news, kudos to the Medical School's Office of Development, which has secured more than $2 million in scholarship funds during the past few weeks. Scholarships help to recruit and retain our brightest and the most sought after students, and yet only 4 percent of our students receive scholarships. Also on the development front, Dr. Stanley Schultz, in preparation for his new role, is convening an advisory committee of basic and clinical scientists to link science and research needs to our philanthropic efforts.
I also want to let you know about a great meeting I had last week with a terrific group of young professionals – members of the UTH Postdoctoral Association. Postdocs often get overlooked in a Medical School no man's land as they are not faculty nor staff nor student. This is a dedicated group with a lot to offer, and I encourage you to support your local postdoc!
I will be meeting with Dr. Kenneth Shine next week regarding the health science center presidential search and will keep you all updated on that front. And next Friday, Dec. 14, is our big day for the Medical School – the ribbon-cutting ceremony for our new research space. All of the health science center school deans will be there as will the former Medical School deans – this is a historic event that you will not want to miss.
Have a great weekend,
For archives of this newsletter, visit the Dean's Communications homepage.


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i just built this gun and am having problems with how to ger the trigger mech in.
if anyone thinks they can help then leave a comment and I'll send you with more pics of how it works.
ITS A CHALLENGE if that helps anyone want to try it...
oh and dont rate it badly because of its looks (handle especially) the actual thing will be three semi moons longer, and the handle will be a proper handle not a junky 1min piece of crap.
its pump looks quite good though.
V1.1 (skip to pic 9 to see it)
I upgraded 1.0 and made the rails so that their is no friction, and their is now an in built TOP LOADING mag which holds six and loads very smoothly. also the pump has been made stronger, and I also put spacers on so when its not loaded, then their is no longer any pressure.
KnexFreEK is still working on the trig mech as I made it very difficult for him lolz.
everyone out there, HAVE A HAPPY NEW YEAR!!!!!!!!!


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Type of Home: Residence
Style: 2 Story
Year Built: 2016
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Address: 3283 23rd NW Av
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Our business guideline 'ISIS' (Innovation Standardization Integration Simplification) regulates our every thoughts and activities. As one stop service orientated, we cooperate with our partners to construct comprehensive supply chain, that greatly facilitate customers' purchase at low cost and high speed. Also, we attach particular importance on flexible distribute network and aim to establish a long term relationship with our clients. Only with this way can the after sales service and delivery time can be guaranteed for our end users.
PRUSP's products include various screen printing equipments, stencil making equipments, drying equipments and related systems. They serve diverse industries from plastic, glass and ceramic to PCB and solar cell printing. Until now, over 40 countries worldwide are using our products. Join us, you will get more.
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About the Responsible Budget Coalition
The Responsible Budget Coalition (RBC) is a large and diverse coalition of more than 300 organizations concerned about state budget and tax issues. It includes organizations that serve children, families, veterans, seniors and people with disabilities; education groups concerned about early learning, K-12 and higher education; labor unions; faith-based and civic organizations; immigrant and refugee families; and many others.
We are a non-partisan, trusted source of information on state budget and tax policy and a leader in the fight to pass a budget that chooses revenue over cuts to vital services.
The individual organizations that belong to the RBC represent a diverse range of interests but are united by these three common principles:
· Adequate revenue to support state priorities and make smart investments
· No more cuts to vital programs and services
· Fairness in raising revenue
Click here for a PDF of a one page overview of the Responsible Budget Coalition.
Membership and Weekly Update
RBC Members: Click here to see a list of RBC members.
Become a Member: To sign up your organization as a Responsible Budget Coalition member click here.
Weekly Update: The RBC sends out a weekly email (usually on Tuesdays) to members and allies, which provides information about the work of the RBC and its members along with news and resources about the state budget. Click here to sign up to recieve the weekly update (if your organization is signed up as a member of the RBC, you should automatically recieve the weekly update).
What is a Responsible Budget?
In short, a responsible budget adequately funds services that promote strong families, communities, and local economies and asks everyone - including corporations and millionaires – to pay their fair share.
Illinois families want a responsible state budget that invests in services that reward hard work, help create opportunity, promote learning, attract businesses and good-paying jobs, promote health and safety, protect the environment, allow children to grow and succeed, ensure independence and dignity for seniors and those with disabilities, and foster thriving communities.
In January 2015, leaders in Springfield let corporate and individual tax rates drop, draining $6 billion from the state budget that pays for K-12 and higher education, human services, environmental protection, and many other critical services. Those tax breaks are primarily going to upper- income individuals and corporations.
We are now many months into a budget impasse. Our state policymakers face a choice between restoring the revenue we lost or allowing more cuts to critical services that strengthen families, communities, and the economy. Below are some examples of what that choice for state policymakers entails. The Governor and lawmakers can...
- close corporate loopholes instead of pricing families out of college and cutting services for sexual assault survivors
- restore some or all of 2014 individual income tax rates - much of which is going to those with high incomes - instead of sacrificing good-paying jobs and critical infrastructure projects
- restore 2014 corporate income tax rates so families can afford childcare and children have afterschool opportunities
- tax high-end services like corporate jet rentals and interior decorating instead of cutting Meals on Wheels and independent living services for seniors and those with disabilities
The Governor & lawmakers need to immediately pass a budget that CHOOSES REVENUE over cuts that harm families, communities, and our economy - they can debate non-budget items later. Illinois can't wait any longer to pass a fully-funded, year-long budget.
Media Contact: Ambar Mentor-Truppa, email@example.com
Field Contact: Jennifer Cushman, Field Coordinator & Policy Specialist 646-326-4279, firstname.lastname@example.com


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Microsoft DevRadio: Making the most out of OData for Your Windows 8 Apps
Download this episode
Mark Stafford from the OData team joins Jerry Nixon on today's episode as they discuss anything and everything to do with OData for your Windows 8 apps. Tune as they take a deep dive into OData sources, its new release strategy and upcoming features.
Next Steps:
Step #1 – Download the Tools for Windows 8 App Development
Step #2 – Download Visual Studio Express for Windows 8
Step #3 – Start building your own Apps for Windows 8
Subscribe to our podcast via iTunes or RSS
If you're interested in learning more about the products or solutions discussed in this episode, click on any of the below links for free, in-depth information:
- Getting started with Windows 8 Apps
- How to Sell Your Apps and Make Money in the Windows Store
- Attend a Windows 8 Developer Camp and Hackathon in your area!
Blogs & Articles:
- Microsoft DevRadio: Managing Your Windows 8 Enterprise Apps with Windows Intune
- Microsoft DevRadio: Your Top Windows Store Questions Answered
Virtual Labs:
Available formats for this video:
Actual format may change based on video formats available and browser capability.
More episodes in this series
Microsoft DevRadio: (Part 13) Practical Azure with Jim O'Neil – Windows Azure Queue Storage
Related episodes
Part 1: Series Introduction
Part 3: Introduction to XAML
Part 7 - Understanding the Navigation Model
Part 4 - Understanding XAML Layout and Events
Part 3 - Introduction to XAML
Part 2 - Exercise: Writing your First Windows Phone 8.1 App
Part 2: Getting Started with the Grid App Template
Part 1 - Series Introduction
Part 17 - Understanding Data Binding, Data Sources and Data Contexts
Part 5 - Overview of the Common XAML Controls
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text: Leaps And BoundsAnna Holmes12/11/09 5:10pmFiled to: Snap JudgmentAlvin ailey american dance theaterDanceGraceRenee RobinsonGettypic6EditPromoteShare to KinjaToggle Conversation toolsGo to permalink [New York, December 10. Image via Getty] Advertisement Renee Robinson of the Alvin Ailey American Dance Theater during dress rehearsal of 'Grace', from the retrospective 'Best of 20 Years', December 10, 2009 in New York. The performance highlights the 20th anniversary of Judith Jamison as artistic director of the company. AFP PHOTO/Stan Honda (Photo credit should read STAN HONDA/AFP/Getty Images)Reply6 repliesLeave a reply


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Hey look trendy mirrors, also I'm pounding your ass while taking a naughty self pic with out camera…. smile!
May 4th, 2010 Naughty Self Pics
2 Responses to "Self Sex Shot in Mirror"
Aaralyn Barra Gets Fucked By two Horny Cocks…
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Search the site
Location: April 1st Rares - Battleon Price: 500 AC Sellback: First 24 Hours: 450 AC After 24 Hours: 125 AC Rarity: Epic Rarity Base Level: 10 Description: Dage turned into a Sneevil for April
Level 2 Level 15 Locations: Boxes HeroMart New Year Newbie Sneevil War (Location) Level: 2 Difficulty: 1 Total HP: 500 396 Attacks: Slash: 17-21 Stab: 35-50 17-21 Temporary Items
: Kiwi is a different kind of sneevil. Never cared much for boxes. So now he's a mercenary for hire. For the right price, he'll fight for you! Note: Also see Dage the Sneevil. Thanks to Kanai_Izumi, Nightly and Reign Man.
Locations: Boxes New Year Newbie Price: N/A (Dropped by Sneevil) Sellback: 250 25 Gold Type: Note Description: Find the dragon in Greenguard. Slay the dragon. Follow the instructions you find
Locations: Doomwood Forest Necro U Price: N/A (Dropped by Doomwood Treeant) Sellback: 0 Gold Type: Note Description: Go back to boxes and slay the sneevils until you find a box cutter. Bring
Locations: Boxes New Year Newbie Price: N/A (Dropped by Sneevil) Sellback: 0 Gold Type: Resource Description: Cuts boxes. Notes: Used in the 'Dage's Black Box' quest. You can equip this item (replaces your helm). Thanks to .Shadow//. Get this item in our free web game at www.AQ.com!
Fools Day 2013 Dark Lord of the Undead Legion Dage the Sneevil?! April 1st release I have evolved a new form… a shorter, more… boxy form. It is only when my birthday
Dropped by Sneevil (Level 2) Rewards: 0 Gold 0 Exp You may also choose one of: Dage's Desolation G Amethyst of Thorns Blinding Edge of the Good Twisted Crystal Staff GalactaBot 1.0 Plasmic
Jump to: Shadowfall, Evil War Dage, Sword of the Legion, Ravenscar, Battleon, Under Void, Under Void Bonus Quests, Under Void Daily Quests, Chaos Lab, Infernal Caladbolg, Shadow Legion Vampire
(Dropped during the 'Dage's Locked Box' quest) Parchment (Dropped during the 'Love Letters of Solitude' quest) Sneevil Convinced (Dropped during the 'HeroMart Defender' quest) Items Dropped: Box


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10 Best *FREE* Windows 8 Apps for Kids (*updated)
Posted by admin as Apps, Kids, Microsoft, Windows, Windows 8
There's something so intuitive and simple about touching things on the screen that they just dive in without needing to be shown how it all works.
The problem is that the Windows Store can be a confusing place if you're looking for the best apps for kids. It's hard to tell the good from the bad! So I've compiled a list of my favorite apps that take advantage of the bigger tablet screen while helping to develop skills for your kids.
So, without any further ado, here are my ten best Windows 8 apps for children:
- Paint 4 Kids
A simple app for your kids to paint and color. Help your child to select one of the available drawings and then select you preferred color. Try to fill all the white spaces as you like. You can choose different modes for drawings: fill a shape with a single tap, move fingers to fill a shape or free drawing, so you can write on the entire screen! And of course you can choose the size of your brush. You can create amazing drawings with kids and after the hard work you can save, share and print you artwork with grandparents and friends.
- Sticker Tales
Sticker Tales is a fun educational game that lets kids use their imagination to create pictures and play games as easily as putting stickers in a book. It includes the "Farm Fun" sticker pack absolutely free, with many more sticker packs available for purchase.
- The Magic Farm
Welcome to the Magic Farm, where you will find great adventure, new friends, and get in touch with brazilian folklore in a fun and interactive way. Hear the narrative or create your own recording, while touching and playing with scenario objects and the farm animals.
- ABC & Me Free
Discover the cutest little app for curious babies and playful toddlers. ABC & Me is an app designed for the most tapful experiences and helps for the development of fine motor skills, and hand eye coordination all while having fun when learning.
- AlphaPop
AlphaPop uses bright graphics, fun sounds and an easy gaming environment to help your young children get introduced to their ABC.
- MerlionWords
MerlionWords is a multilingual education application to enhance your Chinese, English, French, Japanese, Korean and Spanish word recognition, writing, and pronunciation skills.
- Daily Colorbook
Play and learn while you and your kids color various motives in our beautifully designed application. With broad content ranging from classic coloring pages to easy learning exercises and dot puzzles, Daily Colorbook is a fun and educational coloring application for all ages.
- NumberPop
NumberPoP uses bright graphics, fun sounds and an easy gaming environment to help your young children get introduced to Numbers.
- My Games
"My Games" is a "7 in 1" game for preschool kids.
- . Kids Connect the Dots Xmas
Keep the kids quiet this holiday season with a festive-themed, musical version of the classical game. Almost a million children have already enjoyed its friendly themes, bright illustrations, and encouraging vocal cues. Newly available for the Windows Phone, this app features 40 simple connect-the-dot puzzles and promises to keep your little one enthralled.
- (23) Comments
- Tags: Apps, Store, Windows 8


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Young Scientists Club The Magic School Bus: Soaring into Flight takes kids on a magical flight. Ms. Frizzle and her students take aspiring aviators soaring into the sky with a dozen dynamic experiments. From building a balloon-jet to launching rockets, gliders and parachutes, Soaring into Flight lets children's imagination soar high along.
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See Maps and directions for help getting to and around LSE.
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Welcome to LSE Health and Social Care.
LSE Health Director Elias Mossialos to lead evaluation of Austrian social insurance system
LSE Health Director, Elias Mossialos, has been engaged by the Austrian Ministry of Labour, Social Affairs and Consumer Protection to lead an evaluation of Austria's social insurance system. The project, which began in late 2016, will evaluate key components of the Austrian system in order to develop a range of policy options aimed at improving the efficiency and quality of services available. Read more...
Complex patients: How health care must adapt to their needs
Reliance on hospitals in OECD countries is poorly suited to the needs of complex patients, who require access to a variety of providers. Elias Mossialos and Martin Wenzl argue that multimorbid patients require a holistic approach to care that helps them access the right services. Read more...
Value in cancer drug spending: new LSE Health research
New research by LSE Health's Sebastian Salas-Vega and Elias Mossialos questions the value of spending on ever-more expensive cancer medicines. The study, published in JAMA Oncology, finds that while most new cancer drugs approved between 2003 and 2013 extended overall survival or improved the quality of life of cancer patients, their clinical benefits vary widely. Read more...
LSE Health signs MOU with leading Chinese Cardiovascular Centre
LSE Health and the National Center for Cardiovascular Diseases (NCCD) have signed a Memorandum of Understanding in the presence of the UK Secretary of State, Jeremy Hunt and the Chinese Vice Minister, Cui Li. The signing took place during a high level Health Policy Dialogue between the UK and China held in Beijing. Read more...
LSE Health awarded major European grant for big data project
LSE Health has been awarded its first major European big data grant. From January 2017 it will coordinate 36 organisations in a public-private consortium with a total budget of 7.2 million Euros. As the leading academic partner, LSE Health will play a prominent role in developing the Innovative Medicines Initiative's "Big Data for Better Outcomes" (BD4BO) programme strategy. Read more...
Childhood bullying places 'long term strain' on UK mental health services
New research shows that childhood bullying has a strong link to mental health service use throughout a person's life, putting additional strain on an "already overstretched" UK healthcare system. A study by the London School of Economics and Political Science (LSE) and King's College London, tracking mental health service use among more than 9000 people over a 40-year-period using data from the 1958 British Birth Cohort, provides unequivocal evidence of the link. Read more...
Title TBA
Date: Tuesday 24 January 2017
Time: 12:45-13:45
Venue: TW2.9.04
Speaker: Professor Peter Smith (Imperial College London)
Signup: http://lsehscformalsmith.eventbrite.co.uk
Inaugural International Health Policy Conference 2017
Date: February 16-19 2017
Venue: London School of Economics (room TBC)
Details: LSE Health & Social Care and the LSE's Department of Social Policy announces a call for papers for the inaugural International Health Policy Conference, to be held at the LSE from 16th -19th February 2017. This unique conference seeks to bring together academics and policy-makers from a wide range of disciplines to take a multi-disciplinary approach to key health and social care issues.
Find out more and register here.
Date: Wednesday 26 April 2017
Time: 12:45-13:45
Venue: Alumni Theatre
Speaker: Professor Matthew Sutton (Manchester)
Signup: https://lsehscformalsutton.eventbrite.co.uk
Big Issues in Health Policy
Date: 28-30 April 2017
Venue: The Ritz Carlton Hotel, South Beach, Miami
Details: This event is a CME accredited event. The event will cover:
The event will bring together professionals from the healthcare industry, and will feature speakers who are prominent academics from the London School of Economics and Political Science and MDs from Cleveland Clinic.
Find out more and register here.
Date: Wednesday 10 May 2017
Time: 12:45-13:45
Venue: Alumni Theatre
Speaker: Professor Marjon van der Pol (Aberdeen)
Signup: https://lsehscformalvanderpol.eventbrite.co.uk
The latest issue of Health Economics, Policy and Law is now available online
In this issue there are articles on competition in China's public hospital system (by Jay Pan, Xuezheng Qin and Chee-Ruey Hsieh), on medical disputes in Chinese public hospitals (by Alex Jingwei He and Jiwei Qian), and on how the pharmaceutical industry influences prescriptions in Shanghai (by Wei Yang). There is also an article on the health service system for native Americans in the United States (by Tiffany Henley and Maureen Boshier).
The issue finishes with a debate section led by Tony Culyer on the use of cost-effectiveness thresholds in health care, with responses from Anthony Harris, and Michael Gusmano & Gregory Kaebnick.
New Eurohealth, Special Gastein Issue on Demographics and Diversity in Europe
Eurohealth Volume 22 Number 3
This issue's Eurohealth, which addresses many topics covered in the 2016 European Health Forum Gastein on the topic 'Demographics and Diversity in Europe: New Solutions for Health' is published by the European Observatory on Health Systems and Policies (in which LSE Health is a partner). Health leaders from the EU, WHO/Europe, Austria, Slovakia and other important institutions present their views and ideas. The Observer section covers: Reconciling demographics and diversity, Inter-culturally competent health care, Health literacy, Innovative solutions for health, Dementia, and the Life-course approach and intersectoral action. The International Section contains articles on: Life-course vaccination, Innovation and patient benefit, Hearing loss, and Health priorities of Slovak EU Presidency. The International Section contains articles on: Refugees and German hospital care, and Big data for health services research.
New Eurohealth, Special issue on priorities for health systems strengthening in the WHO European Region
Eurohealth Volume 22 Number 2
This special issue, marking the 20th anniversary of the Ljubljana Charter on Reforming health care in Europe, published by the European Observatory on Health Systems and Policies (in which LSE Health is a partner), explores how Member States are strengthening their health systems in line with the recently launched strategic document "Priorities for health systems strengthening in the WHO European Region 2015–2020: walking the talk on people centredness". Articles include: Introducing the issue; Priorities for strengthening people centred health systems; Transforming health services to meet the health challenges of the 21st century (Germany, Scotland, Republic of Armenia); Moving towards universal health coverage and a Europe free from impoverishing out-of-pocket payments; Enhancing the health workforce (Wales, Republic of Kazakhstan, Malta, Hungary); Ensuring equitable access to cost-effective medicines and technology (Denmark, Republic of Moldova); Improving health information and health information systems' (Portugal, Slovenia, the Observatory); and Publications.
New Eurohealth on The changing role of nursing
Eurohealth Volume 22 Number 1
Edited by Sherry Merkur, Anna Maresso and David McDaid, this issue's Eurohealth published by the European Observatory on Health Systems and Policies (in which LSE Health is a partner), examines the changing role of nursing, including articles on the state of nursing in the European Union, nurse migration, EU accession and nursing, and whether there an EU framework for nurse education. Other articles include: Health priorities of the Dutch EU Presidency; Implementation status of the cross-border care directive; Making sense of EU health law; Managed entry agreements in the Baltic countries (Estonia, Latvia and Lithuania); What does the Euro Health Consumer Index tell us; and Eurohealth Monitor.
The latest issue of Health Economics, Policy and Law is now online
This issue starts with original research articles on health care mergers (by Jeroen Postma and Anne-Fleur Roos), on switching in health insurance markets (by Daniëlle Duijmelinck and Wynand van de Ven), on the impact of Dutch health care reforms (by Hans Maarse, Patrick Jeurissen and Dirk Ruwaard), on prescription drug costs in the EU (by Eleanor Brooks and Robert Geyer), and on the regulation of primary care in the US (by Michael Richards and Daniel Polsky).
There is then a response from David Chinitz and Avi Israeli to a previously published article on health care in Israel that was written by Dani Filc and Nissim Cohen, and a response to the response by the original authors.
The issue finishes with a review article by Paul McCrone of a book on the rise of ADHD treatment that was written by Stephen Hinshaw and Richard Scheffler.
The issue can be found here.
New Eurohealth on Providing emergency medical care
Eurohealth Volume 21 Number 4
Edited by Sherry Merkur, Anna Maresso and David McDaid, this issue's Eurohealth - from the European Observatory on Health Systems and Policies - addresses emergency medical care. Its Observer section looks at the challenge of providing emergency medical care, including articles on out-of-hours primary care and demand for emergency medical services, urgent care and the English NHS 111 experience, and waiting time policies in the health sector. Other articles include: Challenges and concerns in the new era of EU health policy; Variable implementation of eHealth services within the EU; Medicrime Convention to fight against counterfeit medicines; National men's health policies (Ireland); Hospital reforms (Switzerland); and Eurohealth Monitor.
Listed below are the three most recent blog posts from the LSEHSC blog:
Direct payments in residential careby Daniel Lombard Direct payments have been extended to people living in residential care in England for the first time, in a pilot programme initiated by the Department of Health (DH). The payments are one method of delivering personalisation, an approach towards public services where support is tailored to the needs and preferences of individual people, in order to promote […]
Autism through an Arts Lens: Comments on PSSRU's LSE Literary Festival Sessionby PSSRU organising team Autism through an Arts Lens takes place on Wednesday 22 February, 7.00-8.30pm in the Sheikh Zayed Theatre, New Academic Building, LSE. This event is free and open to all. Tickets will be released on Tuesday 31 January. Over the last week we have received some useful comments and queries on our session on Autism through an […]
Value in cancer drug spending: assessing the clinical risks and benefits from a decade's worth of innovationby Sebastian Salas-Vega and Elias Mossialos There are growing questions about the value from spending on what seem like ever-more expensive cancer medicines. Rising expenditures may make it difficult for patients to access or remain compliant with life-extending therapies. Yet, some have argued that high prices may be justified if new and innovative treatments offer significant clinical benefits. Even as […]
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Product Details
Style: 112826
Please Note: This style is from a previous season
The Spyder Paisley Ski Sock is a warm and cushy sock for kids. Made with a blend of synthetic materials, the Paisley sock cushions and insulates little feet. A custom woven design ups the appeal by making them fun to wear.
- 62% polyester/ 19% nylon/ 19% elastane knit
- Ribbed ankle and instep to help sock stay put
- Seamless toe construction for comfy feel
- Padded shin for extra comfort in ski boots
- Fun Spyder flower design
Questions and Answers
ASK A QUESTION Got questions about this product? Ask them here. Got questions about anything else? Please see our FAQ.
"What size should I order for a 12 year old girl size 5 shoe?"
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Question by Cindy on 9/20/2011 - 9:47 pm
"For a girls size 5 you would need a size Large in kids or a women's size Small. I would recommend the women's size Small so that you could use it for a longer period of time!"
Answer by Sarah (Peter Glenn Staff) on 9/21/2011 - 3:54 pm
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condition: Ex-Demo. Slightly worn product tested or played by endorsing artists. 12 months warranty.
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Regional North America United States Arizona Government Federal US House of Representatives Kyrsten Sinema [D-9]
U.S. Representative for Arizona.
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Congresswoman Kyrsten Sinema
Official site for U.S. Representative Kyrsten Sinema (AZ-9), Democratic party.
Arizona State Legislature - Kyrsten Sinema
Official Arizona government site provides her past legislative profile as a state representative and senator, including her sponsored bills and committee assignments.
Ballotpedia - Kyrsten_Sinema
Wiki about the Arizona politician, from a libertarian, free-market perspective.
Biographical Directory of the United States Congress - Kyrsten Sinema
Short biography along with political party, years of service in Congress and bibliography.
C-SPAN - Kyrsten Sinema
Videos and transcripts of appearances on C-SPAN.
Congress.gov - Kyrsten Sinema
Information on sponsored and cosponsored legislation. From the Library of Congress.
FEC - Candidate Summary Reports: Kyrsten Sinema
Official campaign finance reports and data from the Federal Election Commission.
GovTrack.us - Rep. Kyrsten Sinema [D-AZ9]
Legislative profile and bill tracking.
IMDb - Kyrsten Sinema
Lists of film and television appearances along with related videos.
On the Issues - Kyrsten Sinema
Provides quotes and background on various international, domestic, economic and social issues. Includes profile, contact data, forum and voter match.
OpenSecrets.org - Kyrsten Sinema
Career profile of campaign funds raised and spent.
PolitiFact - Kyrsten Sinema's File
Truth-o-meter fact-checking of statements by the Arizona politician. A project of the Tampa Bay Times.
Project Vote Smart: Kyrsten Sinema
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Roll Call - Rep. Kyrsten Sinema (D-Arizona)
Profile, election history and collected news reports.
Washington Post - The US Congress Votes Database: Kyrsten Sinema
Features complete voting record along with a brief biography, roles in Congress and financial disclosure statement.
Wikipedia - Kyrsten Sinema
Crowd-sourced encyclopedia article about the Arizona politician.
WorldCat Identities - Kyrsten Sinema
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