text:
What are you willing to go through to get there?
By Dena LeMmons
Like so many of you, I have often asked God to show me his will for my life. I have asked him to use me for his glory, and praying countless times to walk in His divine destiny for my life. Then, one day God asked me, what are you willing to go through to get there? It was during a time when I was stripping my fireplace mantel to its' natural wood that He posed this question to me, and presented me with an interesting and yet challenging revelation.
Naively, I expected the stripping process to be complete within two to three day's tops. So armed with stripping solutions, power sander, floor covering, gloves, and protected eyewear I was set to go. After applying, the stripping solution and allowing it to take effect, like an eager beaver I started scraping away. To my dismay and days later, I was nowhere near completion.
To my dismay, as I removed one layer, it revealed another layer, which revealed another layer, and so on and so on and so forth. I'm sure you get the picture. During the stripping process I also discovered that care and special attention was needed while removing the paint from the mantel edges due to its' detailed carvings. A special sharp edged scraping tool had to be used and the slightest slip could damage the intricate artwork. Frustrated, and ready to give up, I yelled "GOD WHAT IS UP WITH THIS" and he politely answered, you.
Stunned and somewhat bewildered, those words captured my full attention. I listened as He reminded me that my home was built in 1947, and there was 59 years of paint and other chemicals that had been layered on top of the wood, and stripping it down to its' "original state" would be a long and delicate process that would require my patience and endurance. Again, I heard "What are you willing to go through to get there?"
Like you, God stated, I must strip away the years of bad habits, the years of disappointments, the years of anger, the years of fear, the years of frustration, the years of sorrow, and your years of pain, in order to get you where I need you to be. I must strip you of yourself, with nothing left to stand in the way of moving you into your destiny. Again, in His gentle voice he asked me, "What are you willing to go through to get there"?
In a last ditch attempt to ease or divert whatever suffering I thought I was about to go through, I quickly reminded him (as though he really needed to be reminded) of my plans, my visions and dreams. In response, I was reminded, "A man's heart plans his way, but the Lord directs his steps." (Proverbs 16:9 NKJV.) Again, He asked, "What are you willing to go through to get there?" In a bold yet stubborn stance, as though pleading for the mercy of the court, I cried out "God, have I not been through enough already? Your word says, "You will never give me more than I can handle," and as a loving and gentle Father he replied, "I will never leave you nor forsake you." Hebrews 13:5 NKJV
I would love to say that I immediately submitted to being stripped, however, that was not the case for me. I went forward full force presenting my case for trail and tribulation diversion. I pointed to situations where I had gone beyond the call of duty. I provided evidence of my obedience; I went full force in fervent prayer, talking about Elijah, how he prayed earnestly that it would not rain and it did not rain.
I was binding on earth, loosening in Heaven, laying down my petitions in a persuasive manner in hopes for a reprieve. As I gave every reason that my mind could conceive, He listened. Yet in the end, if I was to get where he needed me to be, I had to relinquish my will, my way and myself totally to the Father's gentle stripping of that which through time had layered itself upon me. Was the process painful? Absolutely! Did it break me down? Absolutely! Did I feel lost and alone? Absolutely! However, where there was pain, he gave me peace. When I was broken, he rebuilt me strong and mighty. When I felt lost and alone, he comforted me, carried me, covered me and took me by the hand and loving guided me systematically through my stripping process.
For many of you this revelation may be simplistic in thought, but I guarantee that it is superior in its evidence as he continues to prepare me for my destiny. As far as the fireplace mantel, it remains a work in progress. God loves us so much that he knows exactly what it will take to get us where he needs us to be. He always has our best interest in mind. God is interested in his best for us, not our best interest for ourselves. Therefore, I submit this question to you. What are you willing to go through to get there?
Being confident of this very thing, that he who has begun a good work in you is faithful to complete it until the day of Jesus Christ. Philippians 1:6 NKJV.
© Copyright 2017 dancinginstilettos. All rights reserved.
Status: Finished
Genre: Religion and Spirituality
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Groupon Buying SIte Wordpress Theme
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subset: commoncrawl


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Please enter the e-mail address for your account. A verification token will be sent to you. Once you have received the token, you will be able to choose a new password for your account.
STOCKTON: 6650 Inglewood Avenue, Stockton CA 95207 (209) 478-0800
MODESTO: 3600 Sisk Road, Suite 5A, Modesto, CA 95356 (209) 543-9411
© 2015 Humphreys College


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|Unsliced white bread loaf
|Softened butter
|3 Tablespoon
|Sandwich fillings
|3 Cup (48 tbs) (6 different fillings, 1/2 cups each)
Remove crusts from loaf of bread.
Cut bread in half crosswise.
Carefully slice each half into 8 slices lengthwise.
Thinly butter each slice and spread evenly with filling.
Roll up each slice lengthwise and secure each roll with toothpick.
Continue with remaining bread and filling.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 1531 Calories from Fat 605
% Daily Value*
Total Fat 65 g99.5%
Saturated Fat 27.2 g135.8%
Trans Fat 0 g
Cholesterol 96.8 mg32.3%
Sodium 3296.7 mg137.4%
Total Carbohydrates 202 g67.2%
Dietary Fiber 15.1 g60.4%
Sugars 16.1 g
Protein 30 g59.2%
Vitamin A 344.2% Vitamin C 1206.4%
Calcium 1.1% Iron 0.05%
*Based on a 2000 Calorie diet


text:
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text:
England, Cheshire Workhouse Records (FamilySearch Historical Records)
|This article describes a collection of historical records available at FamilySearch.org.
Access the records: England, Cheshire Workhouse Records, 1848-1967 .
Record Description
This collection will include records from 1848 to 1967.
The majority of the documents are handwritten on printed forms consisting of columns and lines.
The workhouses started out housing the various types of poor separately. The records generated by the workhouses were kept according to the 1834 poor law act. Legislation in 1930 abolished the boards of guardians and passed the responsibilities to local authorities. Additional legislation in 1948 established the National Health Service. These pieces of legislation brought about the development of infirmaries, which eventually led to the national institution of hospitals and asylums, eventually abolishing the workhouses. Workhouses were there for the able-bodied unemployed (and their families), the impotent poor, the elderly, the chronic sick, orphaned children, unwed-mothers, and those mentally ill. Some people were in and out as work was available, while others spent their whole lives in the workhouse. These records cover about 1.7 million names.
The records for the Cheshire workhouses are from 1837 to the closure of each Poor Law Union. Cheshire Poor Law Unions contains further information about each Union.
The records were used by local authorities keep track of the poor and account for monies spent for their living expenses.
Reliability of records is high regarding dates of birth, death, admissions, and discharges. Reliability of names may vary in illegitimate births.
Record Content
Birth records contain the following information:
- Date of birth
- Address
- Name
- Gender
- Father's name
- Mother's name
- Occupation
Death records may contain the following information:
- Date at death
- Name
- Age
- From what parish admitted
- Cause of death
- Where buried
- Gender
Workhouse records may contain the following information:
- Day of the month
- Day of the week
- Name
- Class for diet
- Number affixed to the pauper's clothes
- Parish to which charged
- By whose order admitted
- Date of the order of admission
- If born in the house, name of parent
- Why seeking relief
- How discharged; and if by order, by whose order
- Death notations
Workhouse creed records contain the following information:
- Date of entry
- Date of admission
- Full name
- Birth date
- Religion
- Name of informant
- Number of clothing
- Occupation
- Admitting authority
- Where from or residence
- Why admitted
- Date of discharge
- Address of friends
How to Use the Record
To begin your search, it would be helpful if you knew the following information:
- Name of ancestor
- Approximate year and place of birth
Search the Collection
Fill in the requested information in the initial search page. This search will return a list of possible matches. Compare the information about the ancestors in the list to what you already know about your ancestors to determine if this is the correct family or person. You may need to look at several images and compare the information about the individuals listed in those images to your ancestors to make this determination.
For tips about searching on-line collections see the on-line video at FamilySearch Search Tips.
Using the Information
Use these records to identify relatives that may not be found in parish registers. The infirmaries attached to the workhouses were opened to the whole community in the later 19th century. The infirmaries generated birth and death records.
|FHL Place England, Cheshire items or FHL Keyword England, Cheshire items in the FamilySearch Library Catalog. For other libraries (local and national) or to gain access to items of interest, see England Archives and Libraries.
Known Issues With This Collection
|Problems with this collection?
See a list of known issues, workarounds, tips, restrictions, future fixes, news and other helpful information.
For a full list of all known issues associated with this collection, please read the attached Wiki article. If you encounter additional problems, feel free to report them at email@example.com. Please include the full path to the link and a description of the problem in your e-mail. Your assistance will help ensure that future reworks will be considered.
Related Websites
- British Library - search for Poor Law Union
- Workhouses - Extensive info about conditions in workhouses, with separate pages for each workhouse.
Related Wiki Articles
Contributions to This Article
| We welcome user additions to FamilySearch Historical Records wiki articles. We are looking for additional information that will help readers understand the topic and better use the available records. We also need translations for collection titles and images in articles about records written in languages other than English. For specific needs, please visit WikiProject FamilySearch Records.
Please follow these guidelines as you make changes. Thank you for any contributions you may provide.
Citations for This Collection
When you copy information from a record, you should list where you found the information (often called citing your sources). This will help people find the record again and evaluate the reliability of the source. It is also good to keep track of records where you did not find information, including the names of the people you looked for in the records. Citations are available for the collection as a whole and each record or image individually.
Collection citation:
- "England, Cheshire Workhouse Records, 1848-1967" Index. FamilySearch. http://FamilySearch.org : accessed 2013. Citing Record Office, Chester.
Record citation (or citation for the index entry):
|The citation for a record is available with each record in this collection, at the bottom of the record screen. You can search records in this collection by visiting the search page for England, Cheshire Workhouse Records, 1848-1967.


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How to claim:
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text:
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis
DOI: 10.1186/ar3295
© Takei et al.; licensee BioMed Central Ltd. 2011
Received: 24 September 2010
Accepted: 24 March 2011
Published: 24 March 2011
There has been no report in the literature of a soluble form of interleukin (IL)-18 receptor α (IL-18Rα). In this study, we evaluated the levels and characteristics of soluble IL-18Rα (sIL-18Rα) in the sera of patients with rheumatoid arthritis (RA) and compared these results to control populations.
The sIL-18Rα complex was isolated from pooled human blood serum using an anti-IL-18Rα monoclonal antibody affinity column. The purified sIL-18Rα was then examined using Western blot analysis and used in experiments to evaluate the effects on an IL-18-responsive natural killer (NK) human cell line, NK0. An enzyme-linked immunosorbent assay was developed, and sera from 145 patients with RA, 6 patients with adult-onset Still's disease, 31 patients with osteoarthritis (OA), 39 patients with systemic lupus erythematosus (SLE) and 67 controls were tested, along with levels of immunoglobulin M, rheumatoid factor, anticyclic citrullinated peptide antibody, IL-18, IL-13 and interferon (IFN)-γ. Area under the receiver operating characteristic curve (ROC-AUC) analysis was used to evaluate the diagnostic utility of the sIL-18Rα complex.
The isolated sIL-18Rα complex can be associated with IL-18 and the soluble form of the IL-18Rβ chain. The sIL-18Rα complex bound to the surface to the NK0 cell line, antagonized the stimulatory effects of IL-18 and IL-2 on the NK0 cell line and inhibited IFN-γ production by the cells. The serum levels of sIL-18Rα complex in RA (186.0 ± 33.5 ng/mL, n = 145) and adult-onset Still's disease (98.2 ± 8.9 ng/mL, n = 6) were significantly (P < 0.001) higher than those in the healthy controls (52.3 ± 8.5 ng/mL, n = 67), OA (38.6 ± 5.4 ng/mL, n = 31), SLE (44.6 ± 3.2 ng/mL, n = 39). The serum level of sIL-18Rα complex was not significantly different between RA and adult-onset Still's disease patients. The serum levels of IL-18, IL-13 and IFN-γ in the RA patients were significantly (P < 0.01) higher than in OA and SLE patients as well as healthy controls. ROC-AUC analysis of the serum concentration of sIL-18Rα indicated that it was significantly diagnostic of RA. Moreover, a tumor necrosis factor inhibitor, etanercept, significantly (P < 0.0001) decreased levels of sIL-18Rα in the sera of 29 RA patients 6 months after treatment.
The sIL-18Rα complex could be a potentially useful biomarker for the diagnosis of RA.
Interleukin (IL)-1α and IL-1β have two homologous receptors IL-1 receptor 1, type I (IL-1RI) and IL-1R, type II (IL-1RII). Functional IL-1R is a complex comprising IL-1RI and IL-1 receptor accessory protein (IL-1RAcP) (see reviews in [1–3]). Upon binding of IL-1α or IL-1β, IL-1RI forms a complex with IL-1RAcP and initiates a cytosolic signaling cascade (myeloid differentiation primary response protein (MyD88), IL1R-associated kinase (IRAK) and tumor necrosis factor-associated factor 6 protein (TRAF6), as well as activation of nuclear factor κB (NFκB), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK)). In contrast, 60-kDa IL-1RII functions as a nonsignaling "decoy" receptor. It has been reported that IL-1RI has a soluble form (sIL-1RI) [4], which can be generated by metalloprotease [5]. Soluble IL-1RII (sIL-1RII) is also generated primarily by proteolytic cleavage in response to a variety of stimuli and can attenuate excessive IL-1 bioactivity by preferentially binding IL-1β. In contrast, soluble IL-1RAcP (sIL-1RAcP) is generated by alternative splicing rather than by ectodomain cleavage. The soluble receptors sIL-1RI, sIL-1RII and sIL-RAcP may attenuate IL-1 signaling [5].
IL-18, a member of the IL-1 family, is well-known to play an important role in T helper 1 (Th1) cell polarization. IL-18 can also act as a cofactor for T helper 2 (Th2) cell development and immunoglobulin (Ig) E production [6–9]. Many lines of evidence indicate that IL-18 plays an important role in the pathogenesis of inflammatory diseases of the bowel, heart and lung [8–12]. The IL-18R belongs to the IL-1R family [1], and the IL-18R complex is composed of the IL-18Rα and IL-18Rβ chains. IL-18Rα (IL-1R5 or IL-1R-related protein 1 (IL-1Rrp1)) is the extracellular signaling domain [1, 13], whereas IL-18Rβ (IL-R7 and accessory protein-like (AcPL) or IL-18R accessory protein (IL-18RAP)) is an adapter molecule [1] in the complex. IL-18Rα alone binds IL-18 with low affinity (dissociation constant (Kd) 20 to 50 nM), and IL-18Rβ alone cannot bind IL-18. However, IL-18Rα can bind with high affinity (Kd 0.3 nM) by recruiting IL-18Rβ. Upon IL-18's binding to the IL-18R complex, IL-18 signaling uses the same adapter molecules (MyD88, TRAF6 and IRAK) as the IL-1 family cytokines and elicits similar responses (activation of NFκB, JNK and p38 MAPK) [1]. Both the IL-18Rα and IL-18Rβ chains are thought to be essential for IL-18-mediated signaling [14, 15]. IL-18 binding protein (IL-18BP) is a soluble protein that binds to IL-18 with high affinity (Kd 0.4 nM) and exerts neutralizing activity against IL-18 [1, 8, 16]. Another member of the IL-1 family, IL-33, binds to the IL-1R family ST2 (IL-33Rα) and IL-1RAcP (IL-33Rβ) complex on cell surfaces and induces the production of Th2 cytokines such as IL-4, IL-5 and IL-13 [3]. Soluble ST2 functions as a soluble decoy receptor for IL-33 and blocks IL-33 signaling [17]. These characteristics suggest that the IL-18Rα or IL-18β chain may have a soluble form.
Recently, the cytokine milieu of the joint in RA patients has become well-understood, and data from human clinical trials are now available. Therapies designed to block the effects of inflammatory cytokine tumor necrosis factor (TNF)-α and the action of the IL-6 receptor (IL-6R) are well-known to be effective in many RA patients. Rheumatoid subcutaneous nodules have the features of Th1 granulomas, with abundant expression of inflammatory cytokines, including interferon (IFN)-γ and IL-18 [8]. The level of IL-18 is reportedly increased in both the serum and rheumatoid synovial fluid, as well as in the bone marrow, of patients with RA, juvenile RA, adult-onset Still's disease and psoriatic arthritis [8, 18, 19]. Moreover, recombinant human IL-18 (rhIL-18) is being actively investigated for its potential efficacy and safety in the treatment of RA. Recent data illustrate the importance of IL-18 in the induction and perpetuation of chronic inflammation in RA patients [9, 19].
As yet, no reported study has focused on the sIL-18Rα chain. In the present study, we attempted to isolate and characterize the human sIL-18Rα complex from human serum. We also found that serum levels of the complex in RA patients were significantly higher than those in healthy controls. Our findings suggest that the sIL-18Rα complex could be a potentially useful biomarker for the diagnosis of RA.
Materials and methods
Characteristics of rheumatoid arthritis (RA) patients and control participantsa
Participant characteristics
Control (n= 67)
RA (n= 145)
AOSD (n= 6)
OA (n= 31)
SLE (n= 39)
Mean age, yr (± SEM)
60.7 ± 2.1
56.7 ± 1.2
54.3 ± 9.9
76.3 ± 1.5b
38.8 ± 2.1b
Sex, n
Smoking status, n
Joint damagec, n
Functional classificationd, n
Mean DAS28 (CRP) (± SEM)
4.3 ± 0.1
Mean DAS28 (ESR) (± SEM)
4.9 ± 0.1
Mean HAQ (± SEM)
5.8 ± 0.7
Mean WBC (cell count/μL) (± SEM)
7,307.3 ± 211.0
8,950.0 ± 533.1
5,134.7 ± 304.8
Mean CRP level, mg/dL (± SEM)
2.27 ± 0.22
2.15 ± 1.14
0.08 ± 0.02
Mean ESR, mm/hour (± SEM)
45.0 ± 2.7
40.3 ± 18.6
RF-positived, n (%)
2/64 (3.1%)
106/145 (73.1%)
0/6 (0%)
2/30 (6.7%)
2/39 (5.1%)
Mean RF, U/mL (± SEM)
3.7 ± 1.3
158.0 ± 20.9 b
4.8 ± 2.7
5.4 ± 1.3b
3.8 ± 1.0
CCP-positivee, n (%)
0/59 (0%)
121/145 (83.4%)
1/6 (16.7%)
0/30 (0%)
3/39 (7.7%)
Mean CCP, U/mL (± SEM)
0.3 ± 0.1
261.5 ± 27.2b
22.5 ± 22.3
0.5 ± 0.1b
PSL, n (%)
89/145 (61.4%)
Mean dose, mg/day (± SEM)
5.91 ± 0.32
MTX, n (%)
58/145 (40.0%)
Mean dose, mg/week (± SEM)
6.87 ± 0.34
DMARDsf, n (%)
117/145 (80.7%)
rhIL-18 (catalog no. B003-5) was purchased from MBL (Nagoya, Japan). rhIL-18Rα (IL-1 R5) or Fc chimera (catalog no. 816-LR), mouse antihuman IL-18Rα monoclonal antibody (mAb) (clone 70625; catalog no. MAB840) and mouse antihuman IL-18Rβ mAb (clone 132016, catalog no. MAB1181; and clone 132029, catalog no. MAB118) were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Rabbit antihuman IL-18Rα polyclonal antibody (pAb) [28] was kindly provided by Dr Tsukasa Seya (Hokkaido University, Sapporo, Japan). Antihuman-IL-18 mAb (clone 8 (IgG2a)) was kindly provided by Dr Do-Young Yoon (Laboratory of Cellular Biology, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea [29]). Antihuman-IL-18Rα mAb (H44 (IgG1)) was established in our laboratory [30] and is commercially available from BD Pharmingen (San Diego, CA, USA), eBioscience (San Diego, CA, USA), BioLegend (San Diego, CA, USA) and Serotec (Oxford, UK).
Purification of soluble human interleukin-18 receptor α complex from human blood serum
Mouse antihuman IL-18Rα mAb H44 was used on an anti-IL-18Rα mAb affinity column to isolate the complex. The H44 hybridoma cell line was cultured in serum-free medium (GIT; Wako Pure Chemical Industries, Ltd., Osaka, Japan). Proteins in cell-free culture supernatants were precipitated with ammonium sulfate and then further purified using a protein G column (GE Healthcare, Tokyo, Japan) as reported previously [31]. This purified H44 mAb was coupled to a HiTrap NHS-activated HP column (GE Healthcare) in accordance with the manufacturer's protocol. Samples of pooled human blood serum were then applied to this affinity column. Phosphate buffer (10 mM, pH 6.8) was used as the binding buffer, 10 mM phosphate buffer plus 50 mM NaCl (pH 6.8) was used as the washing buffer, 100 mM glycine buffer (pH 2.5) was used as the elution buffer and 1 M phosphate buffer (pH 8.0) was used as the neutralization buffer. All buffers were filtered through a 0.45-μm filter (Millipore, Tokyo, Japan) before the experiments. Serum samples were diluted twofold with the binding buffer, filtered through a 0.45-μm filter (Millipore) and applied to an antihuman IL-18Rα mAb affinity column that had been equilibrated beforehand with the binding buffer. The affinity column was washed with the washing buffer. The elution buffer was then allowed to flow through the column, and every 1-mL sample was collected into a test tube containing 50 μL of neutralization buffer (fractions collected were denoted in numerical order as fractions 1, 2, 3 and so on), with the fractions being monitored with UV radiation at 280 nm. The collected fractions were dialyzed against distilled H2O (Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan) in the presence of 1 mM phenylmethanesulfonyl fluoride (Sigma) at 4°C. Purified hIL-18Rα complex was concentrated using Centricon centrifugal filters (Millipore), and all of the samples and buffers were then kept at 4°C.
Establishment of enzyme-linked immunosorbent assay system for measurement of serum soluble human interleukin-18 receptor α complex
H44 mouse antihuman IL-18Rα primary mAb dissolved at 4 μg/mL in phosphate-buffered saline (PBS) was dispensed into enzyme-linked immunosorbent assay (ELISA) plates (Nunc, Tokyo, Japan) in aliquots of 100 μL/well and left undisturbed overnight at 4°C to allow it to become immobilized. The plates were then washed twice with 200 μL of PBS buffer containing 0.5% Tween 20, and 200 μL/well of 10% Block Ace blocking solution (Dainippon Sumitomo Pharma Co., Ltd., Osaka, Japan) were added and left for at least 2 hours at room temperature to prevent nonspecific adhesion of the secondary antibody to the plates. The plates were then washed again twice with 200 μL of PBS buffer containing 0.5% Tween 20. Human blood serum samples were then aliquoted at 100 μL/well. rhIL-18Rα protein (R&D Systems, Inc.) diluted to 400, 200, 100, 50, 25, 12.5 and 6.25 ng/mL was used as the standard for the human sIL-18Rα complex ELISA system. After 2 hours of incubation at room temperature, each well was washed three times with 200 μl of PBS buffer containing 0.5% Tween 20. Next, 2 μg/mL biotin-labeled rabbit antihuman IL-18Rα secondary pAb was dispensed at 100 μL/well, followed by incubation for 90 minutes at room temperature, and then each well was washed four times with 200 μl of PBS buffer containing 0.5% Tween 20. This was followed by addition of 100 μL of 0.5 μg/mL streptavidin-bound horseradish peroxidase (Upstate, Tokyo, Japan) to each well, and the plates were left undisturbed for 30 minutes at room temperature. Each well was then washed five times with 200 μL of PBS buffer containing 0.5% Tween 20. 2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ELISA POD Substrate A.B.T.S. kit; Nakarai, Kyoto, Japan) was then added at 100 μl/well, and the plates were left undisturbed for 30 minutes at room temperature, followed by addition of a stop solution at 100 μL/well to stop the enzyme reaction. The amounts of the sIL-18Rα complex protein were determined by measuring the absorbance at 450 nm in comparison with the standard rhIL-18Rα protein sample. The limit of sensitivity of this ELISA system was <5 ng/mL.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis
SDS-polyacrylamide gel electrophoresis (PAGE) was performed using premade 10% to 20% or 15% to 25% polyacrylamide gel (Multigel II Mini; Dai-ichi Kagaku Yakuhin, Tokyo, Japan). The gel was stained with Coomassie Brilliant Blue (CBB) or assessed using Western blot analysis as described in our previous reports [30, 32]. MagicMark™ Western Standard (Invitrogen, Carlsbad, CA, USA) was used for protein band standardization.
Cytokine enzyme-linked immunosorbent assays
Levels of mature IL-18, IL-13 and IFN-γ in serum were measured using ELISA kits (IL-18: MBL, Nagoya, Japan; IL-13 and IFN-γ: R&D Systems, Inc.). The limits of sensitivity of these ELISA kits were 12.5 pg/mL, 32 pg/mL and 8 pg/mL, respectively.
Flow cytometric analysis
The purified sIL-18Rα complex protein and H44 mAb were labeled with biotin as reported previously [31]. Cells were incubated with 2 μg of biotinylated control mouse IgG1 (Caltag, Burlingame, CA, USA), 2 μg of biotinylated anti-IL-18Rα mAb H44 (mouse IgG1) or 10 μg of biotinylated anti-IL-18Rα complex protein at 4°C, then washed with PBS and incubated with streptavidin-PE (BD Pharmingen), followed by flow cytometric analysis as reported previously [12].
Interferon-γ production inhibition assay using a human interleukin-18-responsive natural killer cell line
We used the hIL-18-responsive human natural killer (NK) cell line NK0, a subclone of the original NK92 cell line. Using NK0 cells, we performed IFN-γ production inhibition assay in vitro as reported previously [29, 30]. Briefly, NK0 cells were washed three times and cultured in RPMI 1640 medium with 10% fetal calf serum (FCS) for 18 hours. The cells were then washed once, suspended at 5 × 105 cells/mL in RPMI 1640 medium with 10% FCS and pretreated with sIL-18Rα complex (0.01 to 30 μg/mL) for 10 minutes at room temperature. The cells were then treated with rhIL-2 (50 IU/mL) plus rhIL-18 (50 ng/mL) for 18 hours. Production of human IFN-γ in the supernatants was analyzed using ELISA kits (R&D Systems, Inc.).
Statistical analysis
Data are presented as means ± standard errors of the mean (SEM). Differences between the RA patients and the controls were analyzed by using the Wilcoxon rank-sum test. The sensitivity and specificity for the diagnosis of RA were analyzed using area under the receiver operating characteristic curve (ROC-AUC) analysis generated by logistic regression as reported previously [33]. Statistical analysis was performed using the JMP 7.0.1 software package (SAS Institute, Japan, Tokyo, Japan). Differences were considered significant at P < 0.05.
Isolation and characterization of soluble human interleukin-18 receptor α complex from human sera
We performed Western blot analysis to confirm that the IL-18Rα protein was present in fraction 3. This step showed that anti-IL-18Rα mAb clone 70625 detected a band of approximately 60 kDa among the rhIL-18Rα/Fc chimera proteins (lane 1 in Figure 1B). The antibody detected bands of approximately 50 and 30 kDa in the isolated sIL-18Rα complex (lane 2 in Figure 1B), suggesting that the complex had at least two different sIL-18Rα proteins. Upon IL-18 binding, the IL-18Rα chain forms a complex with the IL-18Rβ chain on cell surfaces [1]. Therefore, we hypothesized that the sIL-18Rα complex associates with IL-18 and sIL-18Rβ proteins, and we investigated this possibility using Western blot analysis. The anti-IL-18 mAb (clone 8) detected a strong band of approximately 30 kDa and a weak band of 50 kDa (Figure 1C). As the IL-18 monomer has a calculated molecular mass of 14 kDa [34], IL-18 associated with the IL-18Rα complex could be dimeric. The anti-IL-18Rβ mAb (clone 132016) detected bands of approximately 60, 40 and 18 kDa in the isolated sIL-18Rα complex (Figure 1D). Furthermore, a hIL-18 ELISA kit detected IL-18 protein in the isolated sIL-18Rα complex (data not shown). These results suggest that the human sIL-18Rα complex might be associated with dimeric IL-18 and the sIL-18Rβ chain.
Ability of the soluble interleukin-18 receptor α complex to bind to the surface of a natural killer cell line
Soluble interleukin-18 receptor α complex prevents interferon-γ production by a human natural killer cell line
We examined whether the isolated IL-18Rα complex protein was able to prevent or increase the production of IFN-γ by NK0 cells stimulated in vitro with IL-2 or IL-18. We reported previously that rhIL-18 proteins synergistically increased the production rhIL-2-induced IFN-γ by NK0 cells [30]. Here we found that the sIL-18Rα complex protein alone did not induce IFN-γ production by NK0 cells and did not synergistically induce IFN-γ production by NK0 cells stimulated with IL-2 alone or IL-18 alone (data not shown). Therefore, we examined whether the sIL-18Rα complex would be able to prevent IFN-γ production by NK0 cells stimulated in vitro with IL-2 or IL-18. Interestingly, IFN-γ production by NK0 cells stimulated with IL-2 or IL-18 was dose-dependently inhibited by the sIL-18Rα complex (0.003 to 10 μg/mL; approximately 70% to 80% inhibition at 10 μg/mL) (Figure 2B). Thus, the sIL-18Rα complex exhibited antagonistic, but not agonistic, activity. In addition, treatment with rhIL-18Rα protein (0.1, 1 and 10 μg/mL) inhibited the production of IFN-γ by NK0 cells stimulated with the combination of rhIL-2 (50 IU/mL) and rhIL-18 (50 ng/mL) by 0%, 2% and 34%, respectively (data not shown). These results show that the inhibitory effect of the sIL-18Rα complex on IFN-γ production by NK0 cells stimulated in vitro with IL-18 or IL-2 is much stronger than that of rhIL-18Rα protein.
Increased levels of soluble interleukin-18 receptor α and inflammatory cytokines in sera of rheumatoid arthritis patients
Tumor necrosis factor inhibitors decreased levels of soluble interleukin-18 receptor α in sera of rheumatoid arthritis patients
In this study, we have demonstrated the presence of the sIL-18Rα chain in human serum. The sIL-18Rα complex is composed of the soluble forms of the IL-18Rα and IL-18Rβ chains and IL-18. The complex was shown to bind to the surfaces of the human NK cell line NK0 and prevented the production of IFN-γ by NK0 stimulated with IL-18 and IL-2 in vitro. Thus our results suggest that the sIL-18Rα complex binds to the cell surface and attenuates IL-18 signaling.
A previous study showed that the molecular mass of IL-18Rα protein isolated from the Hodgkin's disease cell line L428 was 64 to 100 kDa. The DNA sequence of hIL-18Rα encodes the signal peptide (Met 1 to Ala 19) domains 1 through 3, to which IL-18 can bind (Glu 20 to Arg 329), and the transmembrane domain (Gly 330 to Tyr 351) [13]. The predicted molecular mass of the extracellular domain of the hIL-18Rα chain is approximately 36 kDa. These results suggest that hIL-18Rα protein on cell surfaces is highly glycosylated and shows sugar chain heterogeneity [13]. Our SDS-PAGE analysis using CBB staining showed that the sIL-18Rα complex comprised a 50-kDa major band and other bands of approximately 60 to 80, 30 and 15 kDa. Western blot analysis showed that the IL-18Rα protein associated with the IL-18Rα complex had at least two components of approximately 50 and 30 kDa. As our anti-IL-18Rα mAb (H44) recognizes the extracellular domain of hIL-18Rα protein [30], the IL-18Rα protein associated with the IL-18Rα complex would have been derived from the extracellular domain of IL-18Rα. However, the molecular mass of the sIL-18Rα protein associated with the IL-18Rα complex was much smaller than that of the extracellular domain of hIL-18Rα as reported previously on the cell surface [13, 30]. Therefore, the sIL-18Rα protein appears to be glycosylated or shows heterogeneity of its sugar chains.
Soluble cytokine receptors of the IL-1R/Toll-like receptor superfamily are thought to be generated by intramembrane proteolysis and/or alternative splicing. sIL-1RI is generated by the action of metalloprotease [5]. sIL-1RII can be generated by both proteolytic cleavage of receptor ectodomains and alternative splicing events. β-secretase 1 and β-secretase 2 can function as IL-1RII sheddases that cleave the IL-1RII ectodomain at a site adjacent to the α-secretase site [2, 36]. TNF-α-converting enzyme (ADAM17), a transmembrane metalloprotease, is also responsible for the proteolytic release or shedding of IL-1RII [37]. It has been reported that sIL-1RAcP is generated by alternative splicing rather than by ectodomain cleavage [38]. Therefore, the sIL-18Rα chain seems to be generated by intramembrane proteolysis and/or alternative splicing events.
IL-18Rβ shares structural similarities with IL-1RAcP [39]. Like sIL-1RAcP, a short form of the IL-18Rβ (sIL-18Rβ mRNA transcript has been described in the rat [40], as well as in human and mouse [39]. A recent study showed that intravenous administration of adenoviruses encoding sIL-18Rβ promoted collagen-induced arthritis in DBA/1 mice [39]. However, the biological function of sIL-18Rβ is largely unknown. In the present study, we have shown that the sIL-18Rα complex comprises IL-18 and sIL-18Rα and sIL-18Rβ. Two different anti-IL-18Rβ mAb clones, 132016 and 132029 (R&D Systems, Inc.), detected bands of approximately 60, 40 and 18 kDa in the isolated sIL-18Rα complex (data not shown). These two anti-IL-18Rβ mAbs recognize the extracellular domain of the hIL-18Rβ chain. Therefore, sIL-18Rβ in human serum is likely derived from the extracellular domain of the hIL-18Rβ chain generated by differential messenger RNA splicing.
It has been reported that the levels of various inflammatory cytokines and chemokines, such as IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, IFN-γ, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, monocyte chemotactic protein-1 and macrophage inflammatory protein-1β, are increased in the sera of RA patients [41]. Inflammatory cytokines and chemokines, including IL-18, IL-13 and IFN-γ, may play an important role in the pathogenesis and development of RA [8, 9]. In this study, we have shown that the serum levels of the IL-18Rα complex in patients with RA or adult-onset Still's disease were significantly higher than those in healthy controls as well as in patients with OA or SLE. However, the origin of the IL-18Rα complex is still unclear. Therefore, we analyzed the levels of the sIL-18Rα complex in both the synovial fluid and sera in three RA patients (26-, 39- and 81-year-old females). The levels of sIL-18Rα complex in the synovial fluid in these RA patients were 22.1, 30.8 and 6.9 ng/mL, respectively. The levels of sIL-18Rα complex in their sera were 39.8, 23.6 and 16.2 ng/mL, respectively. Thus, the levels of sIL-18Rα complex were not greatly increased in the synovial fluid in these three RA patients. Further analysis is needed to address the origin of the IL-18Rα complex in RA patients.
ROC-AUC analysis revealed that RA patients could be discriminated by the serum level of the sIL-18Rα complex as determined by ELISA. Moreover, the TNF inhibitor etanercept significantly decreased levels of sIL-18Rα in the sera of 29 RA patients 6 months after the treatment. Thus, the sIL-18Rα complex may play an important role in the inflammatory process of RA and adult-onset Still's disease. Our present results suggest that the level of the sIL-18Rα complex in serum has potential clinical application as a biomarker for the diagnosis or differential diagnosis of RA or for the evaluation of disease activity in RA patients treated with TNF inhibitors.
In this study, we evaluated the levels and characteristics of the sIL-18Rα complex in the sera of patients with RA and compared these to control populations. Our study indicates that sIL-18Rα is present in the sera of RA patients and is complexed with IL-18 and IL-18Rβ. The levels detected by ELISA were substantially higher in the RA patients and patients with adult-onset Still's disease than in controls, and modeling using ROC-AUC analysis suggested that this assay might be of diagnostic value.
binding protein
Coomassie Brilliant Blue
- IL-1RacP:
IL-1 receptor accessory protein
interleukin 1-associated kinase
polyacrylamide gel electrophoresis
rheumatoid arthritis
rheumatoid factor
area under the receiver operating characteristic curve
systemic lupus erythematosus
tumor necrosis factor.
The authors thank Dr Howard A Young (National Cancer Institute, Frederick, MD, USA) for editing; Drs J Itoh (Chikugogawa-onsen Hospital, Ukiha, Japan), M Kawahara, H Koga (Keishinkai Hospital, Tosu, Japan) and Y Ohkubo (Chiwata Hospital, Nagasaki, Japan) for providing samples; Dr Tsukasa Seya (Hokkaido University, Sapporo, Japan) for providing rabbit antihuman IL-18Rα polyclonal antibody; and Dr Do-Young Yoon (Laboratory of Cellular Biology, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea) for providing antihuman IL-18 monoclonal antibody (clone 8). We also thank Ms Emiko Kuma, Ms Chie Ohki and Ms Kyoko Yamaguchi (Kurume University, Fukuoka Japan) for their technical assistance. This work was supported by a Grant-in-Aid for Scientific Research (C) (no. 21590977) (to TH) from the Ministry of Education, Science, Sports, and Culture of Japan; a grant to the Respiratory Failure Research Group from the Ministry of Health, Labor and Welfare, Japan (to HA); and by a grant from the Okamoto Satoshi Memorial Fund for Pulmonary Fibrosis Research (Tokyo, Japan) (to TH).
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Personal Finance Resources: STDL offers print and online resources to help you make the best financial decisions for your situation, whether you're investing in stocks or mutual funds, buying or selling a house, creating a budget, looking for the safest bank in your area, or facing foreclosure or bankruptcy. The Reference Desk, located on the second floor of the library, can assist you in finding the Library's resources. Visitors to the Library will find:
- Books on investing and personal finance
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- Reference books and investment newsletters that can be used within the library.
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Buying a Home
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Buying a home should be exciting, fun, and easy. We do more than just find you a home. We educate and guide you every step of the way. At the Reinalda Team, we know the product and can show you any home that's for sale.
We find the things that set your home apart from other homes on the market and work with you on a plan for a successful sale. We know how to look at your home through buyers' eyes. Our team believes you shouldn't have to spend a lot of money to get your house market-ready. We show you how to make your house preferred over the competition resulting in a faster sale and a higher sale price.
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"After having my home on the market for over 2 years, I decided to change Realtors. After our first meeting, I was confident Lori would get my house sold. It was; within 2 months. Lori and her team take care of everything for you."
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text: Pinch to zoom Assorted Chocolate Truffles Birthday Gift by Lushleys £7.50$12.87€8.98$9.61 This item is currently unavailable Favourite Favourite View your favourites we're sorry we're sorry, This item is currently unavailable it will be available on 02-05-2017 quantity buy a gift voucher related categories weddings > more > food & drink > chocolates weddings > living & decorating > parties > sweets & chocolate food & drink > chocolates & confectionery > chocolates > chocolates & truffles by Lushleys Location: London, GB read seller's profile see seller's complete range more items from this seller Mixed Chocolate Strawberries Gift by Lushleys £4.50$7.72€5.39$5.76 Mixed Chocolate Strawberries Birthday Gift by Lushleys £5.90$10.12€7.07$7.56 Milk Chocolate Almonds by Lushleys £4.30$7.37€5.15$5.51 Chocolate Truffles Gift by Lushleys £4.50$7.72€5.39$5.76 view all you may also like PRODUCT DETAILS DELIVERY RETURNS gift wrapping available save for later This is a wonderful gift for your loved one, containing three kinds of beautiful handmade quality truffles. These truffles are one of our bestelling products. Perfect as a gift, or as a treat for yourself! The three types of fillings are: Marsala wine and white chocolate, passion fruit and rum. These truffles have a very high cocoa content. Dark chocolate contains a minimum of 55% cocoa solids and milk chocolate, a minimum of 32%. The bag contains approximately ten truffles. This is a quality product and should not be confused with inferior truffles on the market. (12 truffles). Allergens: soy, milk, eggs. May contain traces of hazelnuts, peanuts, almonds, wheat. made from: Ingredients: chocolate (sugar, cocoa mass, cocoa butter, emulsifiers [soy lecithin, E 476], flavouring vanillin), champ-krem filling (white chocolate [sugar, cocoa butter, whole milk powder, emulsifier soy lecithin, vanilla], cream, sugar, Marc de Champagne, condensed whey, alcohol, milk protein), milk chocolate (sugar, cocoa butter, whole milk powder, cocoa mass, whey powder, milk fat, emulsifiers [soy lecithin, E 476], vanillin), base-crème filling (white chocolate, vegetable cream, sugar, ethyl alcohol, condensed whey, milk protein, emulsifier soy lecithin), chocolate filling (cocoa mass, sugar, emulsifier soy lecithin, natural flavouring vanilla, refined vegetable fat, milk fat), white chocolate filling (white chocolate [sugar, whole milk powder, cocoa butter, emulsifier soy lecithin, natural flavour vanilla], refined vegetable fat, milk fat), cocoa butter, egg yolk filling, invert syrup, passion fruit filling, white chocolate, flavourings. Chocolate contains cocoa solids 55% minimum. Milk chocolate contains cocoa solids 32 % minimum. Nutritional value per 100 g: energy value – 2123 kJ/508 kcal; protein – 6.6 g; carbohydrate – 48.2 g; fat – 32.1 g. dimensions: H3 x W11 x D16 cm. Approximate weight: 140g product code: 232412 This product is dispatched to you by Lushleys. You have 28 days, from receipt of cancellable goods, to notify the seller if you wish to cancel your order or exchange an item. Please note: goods that are personalised, bespoke or made-to-order to your specific requirements, perishable products and personal items sold with a hygiene seal (cosmetics, underwear) in instances where the seal is broken are non-refundable, unless faulty. Read more about our returns policy.Should you choose to return or exchange your order you will need to deliver the item(s) to the UK, where this seller is based. Lushleys's return policy Please see notonthehighstreet.com's returns policy.


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Added to Cart
ASUS 15.6" Core i3-5005U 4GB RAM 500GB HDD Notebook (A540LA-XX289T)
ASUS 15.6" Core i3-5005U 4GB RAM 500GB HDD Notebook (A540LA-XX289T)
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Enjoy a smooth and responsive computing experience with the incredible ASUS 15.6" Core i3-5005U 4GB RAM 500GB HDD Notebook. Designed for performance as well as portability, this stylish notebook is perfect for work or play!
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Levels of Discovery Wild West Revolving Bookcase
- Revolves for easy access
- 12" and 10" shelves hold favorite storybooks
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Product NotesView All Levels of Discovery View All Storage & Organization
List: $174.99
|Wild West Revolving Bookcase
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text: 2010 Shelby GT500 Super Snake, More Serpents Unveiled!Matt Hardigree6/26/09 3:00pm241EditPromoteGo to permalinkWe've been salivating for the updated Shelby lineup ever since the new GT500's unveiling. Well, here it is — the mad-crazy 700HP+ 2010 Shelby GT500 Super Snake, along with the rest of Shelby's new lineup. Galleries and details, below.


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Gallup: Obama's approval rating ranks 10th of 12 most recent presidents
posted at 6:45 pm on July 20, 2009 by Allahpundit
This won't affect the media mantra that America *hearts* Hopenchange but I thought I'd flag it anyway. In fairness to The One, sure, he's third from the bottom, but how many of his predecessors had done as much damage by this point as he has? His Iran policy's gone nowhere, the stimulus is a galactic bust, the annual deficit is creeping up on $2 trillion, and he's poised to ruin American health care by pushing through the mother of all boondoggles — and his numbers are still positive. What does he have to do to get the public to turn on him? "Hike the Appalachian trail"?
The public's confidence in President Obama's ability to handle the economy is eroding amid concern about higher federal spending and expanding government power, a USA TODAY/Gallup Poll finds — a development that could complicate his efforts to push a health care plan through Congress in the next few weeks.
In the survey, taken Friday through Sunday, Americans by 49%-47% disapprove of his handling of the economy, and by 44%-50% disapprove of his handling of health care.
His overall approval rating was 55%, the lowest of his young presidency. That puts Obama 10th among the 12 post-World War II presidents at this point in their tenures. When he took office, he ranked 7th.
Here's a bit from Steele's bid to ride the momentum this morning at the National Press Club. It'll be instantly clear which Luntz-ian buzzwords he's using to tar ObamaCare. The S-bomb was also dropped but it came during the Q&A and thus isn't in the vid.
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- D'oh « DPGI – the aftermath
- the rightwing czar» Blog Archive » Gallup: Obama's approval rating ranks 10th of out of last 12 persidents
- MightyRighty.com - Conservative News, Discussion and Community
- Hot Air » Blog Archive » Another poll shows Obama's approval rating on health care below 50%
- Fox News' Chris Wallace Gently Pwns GOP Rep Mike Pence « Daily Dose


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The Florida Sportsman Expo will return to the St. Lucie County Fairgrounds, April 29th and 30th In Fort Pierce. You will find fishing, hunting, archery, guns, spear fishing, diving, boats, concealed weapons classes and more.
*Right click and Open in New Window to enlarge.
Save $1 on your admission to the Florida Sportsman Expo in Fort Pierce, at the St. Lucie County Fairgrounds, April 29th and 30th. Kids 12 & Under with adult FREE.
Fort Pierce, April 29 & 30, 2017, St. Lucie County Fairgrounds
Tampa, October 7 & 8, 2017, Florida State Fairgrounds
Take advantage of super show specials available only at the Expos for anglers, hunters and boaters of all ages.
An Expo exclusive is the popular Bait, Rigs & Tackle tables where anglers can sit down and watch Florida Sportsman experts show how to tie the best knots and rig the right baits.
Stop by the Lady Angler's Stage, and listen to some of the best women in the outdoor industry share their tips and tactics on multiple topics!
Inshore Seminar Stage sponsored by Rhodan Marine Systems: Hard-core fishing information will be offered by top fishing pros on subjects of interest to local anglers. Sponsored by Rhodan Marine, PowerPoint presentations and demonstrations take place on a flats boat inside the exhibition hall.
The Offshore Stage is actually performed from the cockpit of an offshore boat at the designated seminar area. Each angler in the audience will learn little-known tactics associated with big-game fishing in the region.
Learn the ABCs of the latest craze in stealth fishing — both inshore and offshore — where most other vessels simply cannot go. Techniques, special gear and proper kayaks are detailed so anyone can become a successful kayaker.
Various manufacturer's throughout the industry showing and speaking about their products.
Hunters will enjoy seminars by some of the most successful outdoorsman in the area and this year, for the first time ever, we will have an indoor gun show where you can buy, sell and trade firearms.
Learn the in's and out's of your marine electronics with a visit to the Electronics Stage.
Avid outdoor enthusiasts gather under one roof to attend the weekend shows and to see for themselves all the seminars, demonstrations and booths filled with tackle, gear, boats, fishing & hunting accessories and services.
Florida Sportsman Expo Accommodations


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Raise up to 2.00%.
Supplied with fixed armrests Basic tilt adjustable tension for back and seat tilt Knee tilt-Backrest and seat move at a fixed angle. Tilt point is near the knees for comfortable reclining Ready Assembled Fire retardant... show more
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Article Provides Road Map to Improved Law School Loan Repayment Assistance Programs
For many years, student debt has been a major obstacle for public interest–minded lawyers. Law school based loan repayment assistance programs (LRAPs) have helped make public service work a viable choice for attorneys. However, comprehensive LRAPs are expensive and, without adequate funding, most law school LRAPs provide only modest benefits to a fraction of the public interest lawyers struggling with student loans.
If you want to leverage the federal support of the College Cost Reduction and Access Act (CCRAA) to create or improve a law school based Loan Repayment Assistance Program consider this. In their article Coordinating Law School Loan Repayment Assistance Programs with New Federal Loan Repayment and Forgiveness Legislation, Professor Philip G. Schrag and Assistant Dean for Financial Aid Charles Pruett of Georgetown University Law Center offer a road map for those interested in coordinating law school LRAP benefits with the Public Service Loan Forgiveness and Income-Based Repayment Programs established by the College Cost Reduction and Access Act. The authors review the increased debt burden for law graduates and the efforts of law schools to create Loan Repayment Assistance Programs, and identify policy issues for law schools to consider in light of the federal programs. The authors also created a companion calculator that schools may use to project the costs of new or revised LRAP programs.
The passage of the CCRAA should encourage law schools to evaluate their current LRAPs, consider design changes to ensure that LRAP benefits compliment Public Service Loan Forgiveness and Income-Based Repayment benefits, and recognize the importance of a continued commitment to preserving or enhancing LRAP funding.Back to
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Nottinghamshire England
Barn Garage, Keyworth
Official Data Barn Garage, Keyworth
- Official Name
- Reg. office address
- UNIT 5 DEBDALE LANE, NG12 5HN, KEYWORTH, Nottinghamshire England
- Company Number
- 08223695
- SIC Code
- 45200 - Maintenance and repair of motor vehicles
- Date of Incorporation
- 21 Sep 2012
- Company Type
- Private Limited Company
Description Barn Garage, Keyworth
Barn Garage invites you to Book an MOT online NOW using VMM, our live, 24/7, real-time online MOT Booking System. Barn Garage was established in 1975, and has been commited to being the first choice in MOTs, servicing and repairs. We are totally commited to providing a fast, friendly service with honest advice, backed by our fully trained technicians and quality of work carried out. We are a completly unbiased MOT testing facility operating strictly within VOSA testing guidelines.
Keywords:Garage Services, Car Maintenance, Mot Test, Wedding Cars
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