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Overton's Type IV Boat Throw Cushion
Add comfortable seating and safety to boat
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Webbing straps for throwing or carrying
Personalized 24" Ring Buoy
Personalize life ring with up to 32 characters
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Overton's Floating Telescoping Paddle With Boat Hook
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ACR Safety Whistle
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Attach to life jackets, rafts, foul-weather gear
8-oz. Marine Air Horn
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Nonflammable, compressed gas
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First Alert Marine Compact Fire Extinguisher, 5-B:C
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USCG approved for marine use
Includes mounting bracket
Overton's Convex Ski Mirror
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Life Line Rescue Throw Bag
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Life Vest/Life Jacket Storage Bag
Holds 5 Type II vest or 5 boat cushions
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Life vests and cushions are not included
Stik-A-Flag Water Ski Flag
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Attaches easily to life vest and ring buoys
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Sur-Mark Buoy Labels
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Sold in pairs to cover 1 buoy
Overton's Panoramic Boat Mirror, 4" x 8"
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Weems and Plath SOS Distress Light and Flag
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Orion Coastal Alerter Safety Flare Gun Kit
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Taylor Made Sur-Mark Regulatory Buoy with Label
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Storm Wind Storm Safety Whistle
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Fits comfortably in your hand
20" Ring Buoy
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Orion USCG Approved Distress Flag
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Brass grommets with four 12" nylon ties
Heavy-duty vinyl
Marinco Mini Compact Single Electric Horn
304 stainless steel covers and diaphragms
Stainless steel mounting bracket
Single 12V horn
106 dB rating


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Accueil › Forums › Séries › DROME ARDECHE- 2ème/3ème Séries 2015-2016 › Répondre à: DROME ARDECHE- 2ème/3ème Séries 2015-2016
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subset: commoncrawl


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Nice. I like amoena's and closed standards with lots of substance.
Annette Milch in Lexington, KY. I'm packing to go to the Region 7 Iris Convention & garden tour & show in Jackson, TN tomorrow.
-----Original Message-----
From: Michael Sutton <email@example.com>
To: iris-photos <email@example.com>
Sent: Thu, Apr 25, 2013 9:32 am
Subject: [iris-photos] receeding rim seedling
Same cross as the last post. Also tall with 8
buds, a little plain but exhibits a nice receeded rim.


text:
The topic arose on one of the Iris groups about growing reference collections. Since this is a useful idea pertinent to all types of Iris I would like to plant a seed of this concept in each of the Iris sections and cooperating societies.
In England and in fact in several other countries there has been a movement to create National collections. These collections of living plants are established for many reasons: 1. to insure that plants will be perpetuated and not lost to cultivation.
2. to create a living collection of a small group of plants that can provide plants for comparison and study.
3. National collections can act as repositories of cultivars that have temporarily fallen out of the lists of normal commercial sources. They may also be a source of plants that are no longer available to importation due to war, or other political impediments.
What makes the collections valuable is that the plants they contain are registered yearly with a secretary overseeing the collections. If someone is researching a particular plant the secretary can refer to the garden or gardens that hold this plant in their collections. Often holders of collections become so knowledgeable of the small category of plants they maintain they may publish papers books etc. providing information for other gardeners and scientists.
No one disagrees that plants protected in there native habitat have the best chance of being preserved for posterity. But collections can also serve as another safeguard to endangered species when there native habitat is being destroyed.
I encourage everyone to be aware of the National Council of Conservation of Plants and gardens, a simple Google search will bring you much information. Then ask yourself why is it that the USA is one of the few civilized countries that does not have a parallel organization? And what is the Iris society doing to create comparable structures within their group?


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Sam Ruby <ru...@apache.org>
|Re: [New build system] Status of samples
|Fri, 28 Feb 2003 16:36:28 GMT
Carsten Ziegeler wrote: >> First, my feeling is that if there is a better syntax, I would like >> to simply adopt it. I don't care what the definition of better is: >> technical, community, whatever. >> > So where is the right place to discuss the syntax? In short: Cocoon > has many dependencies but the description for the dependencies is a > little bit confusing (at least for me), and has some shortcommings. > There is for example no common naming scheme for dependencies, so you > can for example call fop either fop, or xml-fop or whatever you want. I'd recommend the firstname.lastname@example.com mailing list. But I am willing to discuss it anywhere. At the moment, the following is effectively the equivalent of an alias: <project name="jakarta-ant"> <depend project="ant" inherit="jars"/> </project> We could make this simpler with a new <alias/> tag that has the same effect. Thoughts? - Sam Ruby
|View raw message


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Local, Federal Authorities Investigate Fatal South Texas Bus CrashFederal investigators and local authorities are working to determine what caused a charter bus to crash in far South Texas, killing eight people and injuring 44 others in a one-vehicle rollover.
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5 Day-Trip Ideas That Your Campers Will Still Be Talking About In SeptemberA charter bus ride to these destinations makes for the perfect day trip for your group of summer campers.
En Route To Game, TCU Student Bus Crashes Injuring 3A charter bus carrying TCU students to tonight's games at AT&T Stadium was involved in a minor accident on Saturday, injuring three.
Friends, Family Remember Victims Of Irving Bus CrashThe Texas Department of Public Safety and the federal National Transportation Safety Board continued to probe the bus wreck Thursday in Irving that killed two people and put 42 others in the hospital.


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text: TabsArticle European Food Safety Authority EFSA Journal: EFSA Journal 2011;9(2):2006 [28 pp.]. doi: 10.2903/j.efsa.2011.2006 Acknowledgements: EFSA wishes to thank the members of the Working Group on Cadmium modelling: Billy Amzal, Alan Raymond Boobis, Diane Benford, Clark Carrington, Eugenia Dogliotti, Lutz Edler, Antonio Mutti and Josef Schlatter (chair of the CONTAM Panel) for the critical review on this scientific output and EFSA staff: José Cortiñas Abrahantes for the support provided to this scientific output. Contact: firstname.lastname@example.com Type: Scientific Report of EFSA On request from: EFSA Question Number: EFSA-Q-2010-01135 Approved: 7 February 2011 Published in the EFSA Journal: 8 February 2011 Last Updated: 10 February 2011. This version replaces the previous one/s. Affiliation: European Food Safety Authority (EFSA) Parma Italy Note: Send Print Read the article on the Wiley Online Library Abstract: EFSA received a mandate from the European Commission (EC) to re-assess the elements linked to the establishment of the health-based guidance value in the opinion on cadmium in food of the EFSA's CONTAM panel (EFSA, 2009a). The CONTAM Unit asked support from the Assessment Methodology Unit to re-assess the elements leading to the establishment of the health-based guidance value in the opinion taking into account a report established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA, 2010) where a different health-based guidance value was concluded. In this scientific report, the methodology used by EFSA and its rationale is described in more detail, and supporting evidence for the need of an adjustment factor to establish a health-based guidance value is provided. A comparison between JECFA and EFSA approaches is presented, as well as a sensitivity analysis in relation to the choice of the toxicodynamic variability function used by JECFA in their approach, to illustrate impact when establishing a health-based guidance value. © European Food Safety Authority, 2011 Keywords: Cadmium, meta-analysis, dose-response model, PK models, benchmark dose, hybrid approach Print on demand Number of Pages: 28


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Mobile tech, unstructured information and cloud computing are among the top security concerns for senior-level IT executives at federal agencies, a survey finds. Jolie O'Dell asks whether these fears are proportional to the risks these technologies carry. O'Dell notes that government chief information officer Vivek Kundra wrote recently that "our policies lag behind new trends, causing unnecessary restrictions on the use of new technology."
Full Story:ReadWrite


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Round Foil Balloon in Gold 17" Diameter
DetailsYour DIY parties and celebrations are not complete without balloons. This gold sphere-shaped foil balloon is the perfect way to add some glam to your wedding reception. Great for decorating your 50th anniversary party, too!
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Hotels in Clovis
Hotel reservation
United States
Hotel rooms in Clovis
There are not so many hotel rooms in Clovis, just 17, still everyone can find an offer to suit his choice. The most common offers in this city are 3 и 4 stars, they count 4 out of 17, and their prevalent location is central district. According to our practice, it is the most profitable to book a hotel in the north of Clovis, as it is the location of the hotels with optimal cost and quality offers along with the cheap hotels. Taking into account the small size of the city, there is no need to dwell hotel reservation on the location: the road to the city center would take less then half an hour, so one can book a hotel in any district of Clovis. The most populated with hotels district of Clovis is the south, 76% of cheap hotel rooms are concentrated here. However, even those who want to live in the center can choose from 1 variants of hotel rooms. Clovis is an ideal place for family and couple, such travellers rate hotels there 7.5 out of 10 on average. In Clovis one can enjoy the beauty of Cannon Air Force Base and Clovis Depot Model Train Museum. Moreover, there is a choice of 3 hotels, located within a walking distance from these attractions, however they might not be the cheap ones. Apart from all these, hotels in Clovis offer their customers laundry service, parking, swimming pool and 24 hours front desk service. The most appreciated amenities in Clovis hotels are location and value. According to the visitors reviews, hotels in Clovis has great location, tasty breakfast and awesome vibe. Take a look at our selection of the cheap hotel rooms in Clovis to make a well-informed choice for your reservation!
Cheap hotel rates in Clovis
Currently Hotellook offers cheap hotels with discounts varying from 5 to 60%. The largest discount found by our users in Clovis was 26%. We collected all discount hotel deals in Clovis together to make it easier for you to find the really cheap one!
discount 26%
Excellent family hotel
4.2 km from the city center
Hotel Fairfield Inn & Suites Clovis
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"We are responsible for those who tamed" - said Antoine de Saint-Exupery.
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Top-3 hotels in the center of Clovis
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It is not a surprise that many guests arriving to Clovis pay great attention to the location of their hotel. In order to save your time, we have prepared a collection of the hotels in the center of this city.
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Clovis hotels with restaurants
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Do you like to eat? We, too :) So we collected hotels Clovis with a restaurant in the same place.
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In order to make your trip to Clovis with children a memorable one, you need to choose the right hotel.
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If you like healthy lifestyle, it is very important to train every morning. One of the greatest ways to do it is to swim in the pool! Here is a collection of the cheap hotels with swimming pool in Clovis.
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Best hotels in Clovis
Hotels in Clovis are spread around the city within the distance of 12.2 kilometers, while 1 hotels are located directly in the city center. Those who are keen on antique should definitely fall in love with
Days Inn & Suites Clovis
, the oldest hotel of Clovis, constructed in 1964. This hotel is located 3.3 km away from the city center. The most popular accommodations in Clovis is
La Vista Inn
located in a 1.9 kilometers from the city center.
The best hotels are here and now. Try them!
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Promise of sale contract
Promise share purchase
Published 6 June, 2016 | By burgueraadmin
What is the time of pricing in a share purchase agreement, taking into account the principles of interpretation of contracts?
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text: EconStor Community: Asian Development Bank Institute (ADBI), Tokyo http://hdl.handle.net/10419/53142 Asian Development Bank Institute (ADBI), Tokyo 2017-02-22T06:33:45Z The impacts of infrastructure in development: A selective survey http://hdl.handle.net/10419/115327 Title: The impacts of infrastructure in development: A selective survey Authors: Sawada, Yasuyuki Abstract: Development economists have considered physical infrastructure to be a precondition for industrialization and economic development. Yet, two issues remain to be addressed in the literature. First, while proper identification of the causal effectiveness of infrastructure in reducing poverty is important, experimental evaluation, such as randomized control trials (RCT)-based evaluation, is difficult in the context of large-scale infrastructure. Second, while micro studies so far have focused on the nexus between infrastructure and certain types of poverty outcomes such as income, poverty, health, education, and other individual socio-economic outcomes, to better interpret a wide variety of micro-level infrastructure evaluation results using either experimental or non-experimental methods, the role of infrastructure should be placed in a broader context. To bridge these gaps, we augment the existing review articles on the same topic, such as Estache (2010), Hansen, Andersen, and White, (2012), and World Bank (2012) by addressing these two remaining issues. First, while forming a counterfactual is often difficult for impact evaluation of infrastructure, engineering constraints beyond human manipulation can allow people to adopt quasi-experimental methods of impact evaluation. Second, evaluators can adopt, for example, a hybrid method of natural and artefactual field experiments to elicit the role of infrastructure in facilitating the complementarity of the market, state, and community mechanisms. 2015-01-01T00:00:00Z Trilemma challenges for the People's Republic of China http://hdl.handle.net/10419/115333 Title: Trilemma challenges for the People's Republic of China Authors: Kawai, Masahiro; Liu, Li-Gang Abstract: This paper first reviews recent developments in exchange rate regimes, capital account liberalization, interest rate liberalization, and monetary policymaking in the People's Republic of China (PRC). It then observes that the PRC's monetary policy autonomy may have been reduced with falling capital control effectiveness and a rigid exchange regime that is still tightly managed against the United States ( US ) dollar. This hypothesis is investigated empirically using both the Taylor rule and the McCallum-like rule to test whether the PRC's money market interest rate and/or quantity of money supply are being increasingly influenced by the US interest rate or reserve accumulation. The paper concludes that there is considerable evidence suggesting diminishing monetary policy autonomy in the PRC. To regain policy autonomy, the monetary authority needs to substantially increase exchange rate flexibility of the renminbi as long as it continues to pursue capital account opening. 2015-01-01T00:00:00Z International spillovers of monetary policy: US Federal Reserve's quantitative easing and Bank of Japan's quantitative and qualitative qasing http://hdl.handle.net/10419/115332 Title: International spillovers of monetary policy: US Federal Reserve's quantitative easing and Bank of Japan's quantitative and qualitative qasing Authors: Kawai, Masahiro Abstract: This paper assesses the impact of unconventional United States (US) and Japanese monetary policies on emerging economies, and explores policy coordination issues to promote macroeconomic and financial stability in developed and emerging economies. The paper first considers a theoretical framework that allows us to analyze the impact of one country's monetary policy on other economies. There are two important theoretical predictions. One is that the greater the positive impact of monetary policy easing on a country's real output, the less its beggar-thy-neighbor impact on other countries. The other is that news on future changes in monetary policy can affect exchange rates and stock prices today as financial markets are inherently forward looking. The paper then examines the impact of the US Fed's QE policy on emerging economies, including the introduction of QE, the expectation of its tapering, and the anticipation of an eventual hike in the interest rate. It also discusses the implications of "Abenomics," particularly qualitative and quantitative easing (QQE) by the Bank of Japan (BOJ), for Asian emerging economies. It finds that the impact of BOJ QQE has been positive and, in contrast to US QE1, has not created negative consequences for emerging economies. The paper finally explores policy implications for both developed and emerging economies and suggests policies to be adopted at the country, regional and global levels, emphasizing the importance of communication among central banks and with the market and the need to strengthen global financial safety nets. 2015-01-01T00:00:00Z SME credit risk analysis using bank lending data: An analysis of Thai SMEs http://hdl.handle.net/10419/115353 Title: SME credit risk analysis using bank lending data: An analysis of Thai SMEs Authors: Yoshino, Naoyuki; Taghizadeh-Hesary, Farhad; Charoensivakorn, Phadet; Niraula, Baburam Abstract: Small and medium-sized enterprises (SMEs) are the backbone of most Asian economies. The main obstacle to the development of the SME sector is the lack of stable finance. Considering the bank-dominated characteristic of economies in Asia, banks are the main source of financing, and the lack of a comprehensive credit rating database has been a bottleneck for SMEs. This paper examines how a credit rating scheme for SMEs can be developed, when access to other financial and non-financial ratios is not possible, by using data on lending by banks to SMEs. We employ statistical techniques on five variables from a sample of Thai SMEs and classify them into subgroups based on their financial health. By employing these techniques, banks could reduce information asymmetry and consequently set interest rates and lending ceilings for SMEs. This would ease financing to healthy SMEs and reduce the amount of non-performing loans to this important sector. 2015-01-01T00:00:00Z


text:
The peptide SpiP of Streptococcus pneumoniae regulates the induction of a complex signal transduction system spiR1spiR2spiH. Distinct alleles of spiP and the receptor histidine protein kinase gene spiH were recognized in different pneumococcal clones. The spi system in strain KNR7/87 is adjacent to a bacteriocin gene cluster encoding putative double glycine-type bacteriocins, immunity proteins, and translocator proteins. A direct repeat element upstream of the spiR1 promoter and another three potential transcription start sites within the bacteriocin cluster indicate that SpiP functions as an inducing peptide for bacteriocin synthesis in S. pneumoniae.
Secreted peptides play an important role in quorum sensing in Gram-positive bacteria. They represent signaling molecules of multicomponent regulatory systems that orchestrate the expression of genes involved in e.g. pathogenicity in Staphylococcus aureus, bacteriocin production in a variety of Lactobacillus spp., or genetic competence and transformation in Bacillus subtilis and Streptococcus pneumoniae (for review, see [1]). A common theme in these processes appears to be a post-translational processing of the signaling peptide [2]. In the case of the S. pneumoniae competence signaling peptide CSP and class II antimicrobial peptides, an N-terminal Gly-Gly cleavage motif serves as recognition sequence for dedicated ABC exporters and accessory proteins which function as maturation protease [3]. The secreted peptide acts as a signal molecule for a histidine protein kinase of a two-component system whose sensor domain typically consists of several transmembrane spanning segments. Activation of the His-Asp phosphorelay system appears to be part of the frequently observed autoregulatory synthesis of the peptide, and is essential for the cell density-dependent manifestation of distinct physiological states.
We have now analyzed the function of a S. pneumoniae peptide SpiP with the typical double glycine leader peptide of secreted biologically active peptides. It is located adjacent to but transcribed divergent from a two-component system related to the ComDE His-Asp phosphorelay [4,5] named SpiR1SpiR2SpiH in the present study. Several SpiP variants exist in different pneumococcal clones that are associated with distinct histidine kinase genes SpiH. The in vitro synthesized peptide induces expression of the spiR1spiR2spiH operon. We propose that an imperfect direct repeat located upstream of the spiR1 promoter and potential transcription units in an adjacent bacteriocin cluster serve as recognition sequence for SpiR-controlled gene expression.
Materials and methods
Bacterial strains and culture conditions
The following S. pneumoniae strains are members of genetically distinct clones (serotypes): R6 (non-encapsulated) [6]; 669 (19) [7]; SA9 [original No. 10760] (6A), Sp670 (6B), Sp628 (9V), 637 (23F), Fi2301r [original No. 43351] (23F) [8]; Hu11 (19A), F4 (23F), F1 (23F) [9]. Streptococci were grown in a semisynthetic medium containing 0.16% glucose supplemented with 0.1% yeast extract at 37°C without aeration [7]. Escherichia coli strains were grown in LB broth at 37°C with aeration.
Construction of lacZ reporter fusions
The upstream region of the spiR1/spiR2/spiH genes was amplified by PCR using oligonucleotides 5′-ACTTGGTCAAAGTGGAAGCGGTC and 5′-TAGACTGTTTTCTGCGTCTG covering the −558 to +38 region from the 3′-end of the BOX element to codon 13 of spiR1. The fragment was cloned first into the E. coli plasmid pCRII™ (Invitrogen, Groningen, The Netherlands), excised with BamHI and EcoRV and subcloned into BglII- and SmaI-digested pEVP3 [10] to create a lacZ transcriptional fusion. The resulting plasmid pPRO2 was transformed into E. coli InvαF′ (Invitrogen), and recombinant E. coli clones were selected at 30 μg ml−1 chloramphenicol.
Insertion duplication mutagenesis
Transformation of streptococci followed published procedures [7]. The plasmid pEVP3 and accordingly its derivative pPRO2 cannot replicate in S. pneumoniae, but contains a selectable chloramphenicol resistance marker. Chloramphenicol-resistant transformants of S. pneumoniae can only arise by homologous recombination between the insert and the chromosome, an event by which the cloned region becomes duplicated after integration of the plasmid into the pneumococcal chromosome [10]. After transformation with pPRO2, chloramphenicol-resistant colonies of S. pneumoniae R6bgaA::ermB defective in the β-galactosidase gene bgaA[11] were selected at a concentration of 3 μg ml−1 chloramphenicol. Ten independent transformants were isolated and insertion of the plasmid into the target region verified by PCR analysis.
β-Galactosidase assay
β-Galactosidase was assayed essentially according to published procedures [10]. Hydrolysis of ONPG was monitored at 30°C, and enzyme activity calculated according to Miller, using Nephelo units instead of OD620 of the culture [12]. Chemically synthesized SpiP peptides as deduced from the DNA sequence (Schneider-Mergener, Berlin, Germany) were added to growing cultures at the concentrations stated in Section 3.
DNA techniques
DNA was amplified by PCR with Goldstar Polymerase (Eurogentec, Seraing, Belgium). Sequencing was performed with the ABI Prism™ Dye Terminator Ready Reaction Kit (Perkin-Elmer). For amplification of the 4642-bp fragment covering the region from spiR to spiB, oligonucleotides 5′-CCGTCTCTTGCAGTGCCACCG and 5′- TCCAACAAGTCATGAGCTTCTCCAG were used. Restriction enzymes were obtained from NEB (Schwalbach, Germany). All DNA techniques followed standard protocols [13].
Database searching
Sequence database searches were carried out using BLAST [14] and FASTA [15]. Sequence alignments were constructed using the GCG 9 package or Clustal X 1.8. Sequences for microbial genomes were obtained from The Institute of Genomic Research website at http://www.tigr.org.
Nucleotide accession number
The spiP and spiR1/R2/H sequences of the different strains have been deposited in the EMBL Nucleotide Sequence Database under the following accession numbers: S. pneumoniae KNR7/87, spi and pnc loci: AJ278419; R6, spiR/H locus: AJ278301; strain Sp670, spiR/H locus: AJ278302; spiP peptide gene, strain Hu11: AJ27827; spiP, strain 669: AJ27826; spiP, strain F4: AJ27828; spiP, strain F1: AJ278300; spiP, strain SA) (10760): AJ278299; spiP, strain Sp628: AJ27825; spiP, strain Fi2301r (43351): AJ27824.
Results and discussion
Sequence variation of the peptide SpiP and the adjacent regulatory system in S. pneumoniae clones
The available preliminary genome sequence is derived from a serotype 4 S. pneumoniae isolate KNR7/87. The arrangement of the regulatory system spiR1spiR2spiH, the peptide spiP and putative ABC transporter and accessory domain for processing and export of peptides containing a double glycine-type leader peptide named spiABCD are shown in Fig. 1. The arrangement suggests that the locus represents another peptide-regulated signal transduction system similar to the com loci which are involved in induction of genetic competence: spiP, spiR2 and spiH are homologues of the comCDE operon, and spiABCspiD are related to the comAcomB locus of ABC transporters. The three genes spiABC encode the N-terminal processing domain, the transmembrane domain, and the ATP binding cassette of a peptide exporter, respectively, and spiD is closely related to comB. The region is preceded by a BOX element, a repeat sequence found in intergenic regions of the S. pneumoniae chromosome [16].
Preliminary attempts to amplify the peptide gene in S. pneumoniae R6 failed when oligonucleotide primers designed according to the available sequence were used. However, a 4.7-kb region including the entire regulatory locus could be amplified in several genetically distinct S. pneumoniae clones and in one S. mitis isolate, and HinfI restriction analyses documented extensive sequence variation of this locus (not shown). Two strains were subjected to detailed DNA sequence analysis of the three regulatory genes and the peptide: the laboratory strain R6, and strain 670 representing the widespread multiple antibiotic-resistant S. pneumoniae serotype 6B clone [8]. The response regulator SpiR2 and the 112-amino acid putative DNA binding proteins SpiR1 were very similar in the three strains examined (Fig. 2A). In contrast, the 3′-end of the histidine kinase spiH encoding the N-terminal sensor domain differed substantially resulting in 12 and 25% variation at the amino acid level. The variable regions in the sensor kinase were located mainly within the putative membrane spanning domains (Fig. 2B). Also, whereas the 3′-end of the peptide gene encoding the double glycine leader was identical except for one codon in strain 670, the 5′-end encoding the C-terminal mature signaling molecule differed completely, resulting in two distinct mature peptide variants with 28 amino acid residues in contrast to 18 amino acids of the reference strain (Table 1). Sequence comparison of the deduced peptide in a total of 10 strains revealed another 28-residue variant (Table 1). Thus, the signaling molecule and the sensor domain of the receptor kinase occur as distinct functional units, similar to the allelic variants described for the comC/comD pair where activation of the receptor kinase requires the presence of the matching peptide ([17,18] and references therein).
|The strains represent distinct clones; nc: non-encapsulated. Bacteriocins: strain KNR7/87. The consensus sequence of Gly-Gly leader peptides is given below (cons.). The arrow indicates the processing site. Asterisks indicate conserved sites in the processed SpiP peptides. The Mr and pI are calculated for the processed peptides.
|The strains represent distinct clones; nc: non-encapsulated. Bacteriocins: strain KNR7/87. The consensus sequence of Gly-Gly leader peptides is given below (cons.). The arrow indicates the processing site. Asterisks indicate conserved sites in the processed SpiP peptides. The Mr and pI are calculated for the processed peptides.
Peptide-dependent induction of the regulatory locus
To analyze the activity and specificity of the peptide, the 18-amino acid residue of KNR7/87 and the 28-amino acid residue R6 variant were chemically synthesized. They were tested using a promoterless lacZ reporter gene fused the 5′-end of the partial response regulator gene spiR1 in S. pneumoniae R6bgaA::erm. The peptides were added to the growth medium, and β-galactosidase activity determined at various time points. Addition of the KNR7/87 peptide had no effect up to a concentration of 1000 ng ml−1. The R6 peptide, however, stimulated lacZ expression in the R6 derivative demonstrating that the interaction is allele-specific. LacZ activity was induced around 10 ng ml−1 (Fig. 3). With fivefold higher or lower concentrations, no induction occurred. The genes involved in this regulatory system were named spi for S. pneumoniae peptide induction (Fig. 1).
Upstream of the spiR1 promoter an imperfect direct repeat (IDR) was found (Fig. 4). The related ComE response regulator has been shown to bind to an imperfect repeat motif in the comC promoter region [19], and repeats are common in promoter regions of other peptide-regulated genes [20]. In Lactobacillus plantarum, the two response regulators have been shown to bind to the IDRs found within adjacent bacteriocin promoters with different binding properties [21]. It is therefore feasible that similarly, both SpiR1 and SpiR2 bind to the repeat sequences and control adjacent genes.
A bacteriocin cluster in S. pneumoniae
Three more IDRs could be identified in the KNR7/87 genome, all of which were located adjacent to the spi locus (Fig. 1): one occurred between two divergent operons encoding the ABC exporter system spiABCD and a cluster of small ORFs; and two repeats were present upstream of adjacent transcripts as proposed in Fig. 1. The distance between the IDR and the start codons of putative SpiR-regulated ORFs as well as their orientation with respect to the ATG varied (Fig. 4).
The ORFs encode a reservoir of peptides characteristic for bacteriocin production [1,22]. One single and two tandem ORFs encode bacteriocin homologues of class II with a typical double-glycine leader peptide [23], followed by small hydrophobic peptides resembling immunity proteins generally located in close proximity to bacteriocins; they were named pncA–pncM for pneumococcal bacteriocins (Fig. 1). Each of the putative bacteriocins had a distinct leader peptide and a glycine-rich mature peptide of 45–66 amino acids in agreement with the general length of antimicrobial peptides between 30 and 60 residues [1], and each contained two cysteine residues (Table 1).
The identification of bacteriocin genes in S. pneumoniae, made possible through genomic analysis, is not surprising, given the fact that they have been observed in many lactic acid bacteria (see [1,22] for review). The spi/pnc locus resembles other class II bacteriocins and their inducing factors described in Lactobacillus sake LTH673, L. plantarum C11, and Carnobacterium piscicola LV17, except that the inducer peptide SpiP is not part of the regulatory transcription unit spiR1spiR2spiH, and that SpiR1 contains only the C-terminal domain of a response regulator, i.e. it cannot be activated by phosphorylation.
Bacteriocin-producing S. pneumoniae may affect not only the growth of pneumococcal strains but other streptococcal species as well. It is tempting to assume that bacteriocin production, i.e. the killing and lysis of other streptococci, could also be beneficial for accessing the gene pool provided by related bacteria.
This work was supported by the Stiftung Rheinland Pfalz für Innovation and the European Community Contract No. BI 04-CT98-0424. The excellent technical assistance of Ulrike Klein is greatly acknowledged. The results were presented at the trap meeting, Kaiserslautern, 12–16 September 1999.


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text: I was in the USAF for 24 years. I mostly served my time as a metrologist with a speciality in the measurement of precise time and frequency. I enjoy reading and researching finance subjects however I do very little active trading and that mostly in conservative, balanced, low expense funds with a view to preservation of capital.


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18 Parks near Gainesville, Texas
Lake Kiowa, Texas 6.95 miles SE
Valley View, Texas 10.04 miles S
Thackerville, Oklahoma 11.61 miles N
Whitesboro, Texas 12.52 miles NE
We did however find 18 campgrounds within 25 miles of Gainesville. You can also find a list of cities with campgrounds or RV Parks near Gainesville in the left hand column.
Gainesville, Texas (map) | (940) 668-4530
Sites: 30 | Latest Rate: $15.00 | 6 Reviews
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May 2015 - $15.00
The hook ups are satisfactory. There is a small botanical garden at the entrance. Very sweet for a lunch date. Close to shopping and fast food restaurants. My son and I stay there memorial weekend. I found the town's people warm and welcoming. It saddens me to say the management of the park was rude. His only concern was payment. The city office was closed for the holiday. A drop box isn't an option for me. He said I was there illegally, and must disconnect immediately. I say BOO to the manager and hooray to Gainsville!!!! There are no showers. I didn't use the toilets. This park is good for a night or two. The highway noises don't bother me, but are loud. - Spector
Jun 2014 - $15.00
This is a very small park, however, considering other parks we rated it a 7. Convenient for visiting family and the amenities met our needs. We enjoyed our stay and would return to this park when in Gainesville. - photophreakpat
Feb 2013 - $15.00
This is a small city park next to the interstate. Close to town. WiFi is available at nearby businesses. There is a public restroom which was moderately clean. No showers. There were several sites in which the water wasn't working, and some which had a constant leak. Check the water before setting up: guess how I figured that out! We did not see a manager and we were here 4 days. Payment is made in a Dropbox. We felt safe here but on our last day, our neighbor's car was stolen in broad daylight. It is unlikely that we would stay here again. There weren't many campers. - Joann26
4321 N Interstate 35 Gainesville, Texas 76240 (map) | (940) 668-1888
Sites: 20 | Latest Rate: $20.00 | 13 Reviews
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Nov 2014 - $20.00
As written in the previous review, it's not a great place to park. There are very few slots available due to electrical issues. Two slots were full of nails I noticed. I would choose to not stay again. Only good for overnight if desperate. It is easy to access near the Interstate. Most of the shops are closed in the Outlet Mall. There is a Cracker Barrel restaurant very near the shops. - ks2spi
Jan 2013 - $20.00
This camp has gone to the dogs. We've been staying here on our trip south for years. It use to be a great little spot to stop, do a little shopping, eat out and continue on our way. It's just a dump now. I had to try 4 different sites in order to get one with usable electric. Maintenance seems nonexistent. Long-term Residents take up most of the usable spaces. We'll be looking for an alternative in the future. Maintenance pays dividends, why can't management get that through their heads??? - DelArmy
Sep 2012 - $20.00
Okay for overnight. Rate now $20. Only three or fours still open at outlet mall although their website indicates otherwise. Appears to be mostly long term residents. Utilities worked. No showers. Easy access and fairly level. No shade trees. - BrendaFleming
11667 East US Hwy 82 Whitesboro, Texas 76273 (map) | (214) 641-2132
Sites: 19 | Latest Rate: $25.00 | 1 Reviews
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Sep 2010 - $25.00
Filthy park: mostly monthlies with junk all around their sites. Lots of dogs running loose and dog waste everywhere. If I were the owner I'd be ashamed to have people see my park. If there hadn't been a storm brewing I would have left this park as fast as possible. - retiredhappy
777 Casino Ave. Thackerville, Oklahoma 73459 (map) | (866) 946-7787
Sites: 164 | Latest Rate: $40.00 | 102 Reviews
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Nov 2016 - $40.00
Very nice park convenient to the interstate. We arrived late in the dark and rain, and there were well-lit signs that guided us to the RV park. We turned into the campground too soon, though, and had a little trouble finding the registration office (which is open until 11pm so we were still able to check-in easily). The sites are well spaced and level side-to-side (we had to un-hitch to get level front-to-back). There is a very nice concrete sidewalk around the outside of the park for walking dogs. As others have mentioned, their pull-through sites are actually two sites with connections on each side, so you can pull in from either direction. We will definitely stay here again if we are passing through the area. - barrettjl
Oct 2016 - $34.00
This is a very neat and clean park. It is close to the world's largest casino, which we visited. It is immense, and fun to see. The only issue I had with the park was that we drove a very short distance to get here, and we could not get an early check-in. Kinda drove me crazy because there were plenty of empty spots. When I returned in the afternoon I was informed that there were only back-ins available. I gave the lady a look, then she remembered that I had been there earlier and voila, a pull through materialized. I think they try to leave as many spots open as they can and have back to backs that double for pull through sites and she didn't want to give it up. Even though there were plenty open overnight. Anyhow, the first night is free if you get a players card and they honor it even if you are staying only one night! Not bad. We did that and then stayed one more day anyway... Had fun. Golf course next door - pretty pricey but pretty. - Tiny Beast
Oct 2016 - $20.00
We have been here several times in the past 5 or 6 years and, once again, were not as impressed as many other reviewers. Depending on how you count the sites (pull through sites can be used as 2 back-to-back sites) the sites are about 50/50 between pull through and back in. Others have described the extremely long, cement sites that, when the park isn't full, can be entered from either end and used as one site even though they were designed as back-to-back sites. Our site was NOT level. There is NO cable or antenna TV but most sites are satellite friendly. The Wi-Fi was pathetic. Right after checking in, I connected on about 3 P.M. By the time I was finished setting up, the connection was lost never to be connected the rest of the day. The next morning, I got on about 8:30 AM. and checked the day's weather. Once I did that the connection was again lost. This level of internet service for a big park at a big casino is NOT acceptable. There are two separate bathhouse/laundry buildings. The laundries have 5 washers and 8 dryers each. Each machine is only a buck, but it took $2 to dry a load. Each bathroom has only 2 sinks, stalls, urinals, and showers. That is not enough for a park this size even if all the sites were FHU. In fact, there are 7 tent sites in the north eastern corner of the park. The staff I dealt with seemed a bit clueless concerning some things about the park. There is a pool, a playground, and an in/outdoor pavilion. This park could be a 9 but the lack of cable, pathetic internet, and non-level sites lowers my ranking. Also, there was NO sign of any recycling. - Jerry S
19691 US Highway 77 Thackerville, Oklahoma 73459 (map) | (580) 276-4411
Sites: 267 | Latest Rate: $7.00 | 45 Reviews
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Dec 2015 - $7.00
Resorts of Distinction discount but paid $2 for pull thru, $3 fifty amp, and $1 for WIFI. Roads to site are asphalt and sites level with hard crushed rock. Staff is super friendly and helpful with free coffee in the morning. Some very long pull thru sites (enough for 38 fifth wheek, truck, and motorcycle trailer) and easy on and off access from I-35. - fulltimehusker
Nov 2015 - $2.00
It has good showers and clean restrooms and a very nice laundry. Sites are level with newly paved roads. Staff was friendly.
Railroad noise was frequent and loud. Interstate highway noise was negligible. I would camp here again in a pinch but there are better options nearby.
Rate for ROD is $0 but they add $2 per day for 30 amp. - Morpauwe
Oct 2015 - $18.00
Good power, good water many sand spurs everywhere. RPI members beware of rate. $13.00 base RPI rate includes utilities but then they add $5.00 for 50 amp and $2.00 if you want a pull-through. 30 amp is $3.00. So for a RPI member it is at least $16.00 for a 30 amp site and $18.00 for a 50 amp site. I did complain to RPI. - rjsupersonic
33912 US-82 Whitesboro, Texas 76273 (map) | (469) 500-4361
Sites: 16 | Latest Rate: $15.00 | 1 Reviews
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Apr 2011 - $15.00
We stayed here for a weekly rate of $85 plus electric while looking for the best location to new job. Owners/operators were very nice and friendly. Some sites are just too close to busy road and shaded sites further back were only 35 amp. There are at least 2 large pull thrus and 3 larger back ins with 50 amp. Nice clean sites. Would stay here again, but for a short stay only. - Marslo
1690 E Lone Oak Rd Valley View, Texas 76272 (map) | (940) 726-6354
Sites: 26 | Latest Rate: $27.00 | 3 Reviews
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Apr 2015 - $27.00
Hookups good. Laundromat clean, $1.00 each. Felt safe. Showers great. Lots not level. I had view of lake, and boat repair lot. Was noisy, but bearable. Management uses texting which I liked, convenient. I would stay again. - Spector
May 2011 - $27.00
This was a good overnight stop. It is near Roy Roberts Lake, though, so I suppose it has some possibilities for water activities. There were quite a few construction workers staying here. The sign to the park is missing, so we entered the 2nd entrance by mistake. So we had to back in to our pull through site. No big deal as there was room, but could be better marked for the entrance. Plenty of room to walk the dog. We never saw the owner but she had given us our site number ahead of time when we called for a reservation. GREAT Verizon cell service here and my air card was extremely fast. Gravel sites are level with concrete small patio and table. Nothing fancy but okay amenities for an overnight. We'd stay again if traveling through this area. - Danni
Jun 2007 - $30.00
Large pull throughs and back ins. Cable TV advertised but not available. Posts have cable TV and phone hookups but they do not work. Spaces are tight with slide outs. Has a view of lake. Park full of permanent trailers. Some old. Honors TACO and Passport America. Passport America not honored on weekends. Nothing more than a trailer park with very few travelers. - Tigggerdad
100 PW 4153 Valley View, Texas 76272 (map) | (940) 637-2294
Sites: 104 | Latest Rate: $25.00 | 13 Reviews
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Jan 2017 - $25.00
Check in was quick and easy, and the young woman was friendly. There are some pull through and many back in sites. Most sites have some slope to them, but nothing insurmountable. All sites have good separation, and the shady ones also have vegetation in between, adding to the sense of privacy. Our favorite was site #12, which was right on the lake. Utilities were well placed and worked well. Picked up many OTA TV stations, and Verizon coverage was good. No wifi at site. Park has both concrete and dirt paths, and a swimming area with a nice sandy beach. - BrentB
Mar 2016 - $17.00
This is a hidden jewel in North Texas. It's part of the state park system. While we were there the park was not heavily occupied and rather quiet. The spots are quite separated so that you don't feel cramped. The 50 amp spots are all pull-through. The 30 amp spots are back-ins, and quite a few of them back up to the lake. All spots had a fire pit and a picnic table.
There are several play grounds, bathing houses, and a large public 'beach area'. There is a walking path through the woods which loops throughout the park.
If you pay for the Texas State Parks Pass, you get a good discount. It's good for a year at all of the state parks. - mpruet
Sep 2014 - $26.00
A real hidden gem in this area. The lake is up and there is a lot of activity on the lake, there are two major sights on the lake and Johnson Branch is on the north side and has about a 10th of the traffic as the south side. The JB has about 100 sites and I think about 11 are 50 amp all of those are drive thru. The best sites are all 30 amps and back in but since it was in the 90's we parked in one of the 50 amp pull thru sites to stay cool. Rangers were personable and friendly especially since it was our first time to camp here, we went ahead and bought an annual park pass and paid $26 a night. All of the sites have water and electric and a sewer dump is close to the entrance. About the only negative is w/o the pass it is an additional $7.00 per day per person so the cost gets too high. Gates are locked from 10PM to 6AM but they give you a code so we felt very secure and during the day it was quiet and after dark it was completely quiet. We will be back again and again. - DKRITTER
13037 I-35, Sanger, TX Sanger, Texas 76266 (map) | 940-458-5979
Sites: 168 | Latest Rate: $13.00 | 23 Reviews
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Nov 2016 - $13.00
We stayed here for a month. There were some old rigs even though the rules for monthly residents state no rigs over 10 years of age are allowed. The sites with those rigs were full of junk. One rig was even towed in and parked. Many residents did not pick up after their dogs. The trash can in the dog park was overflowing. Workers were rarely seen. The area for the short-term campers is much nicer than for the long-term campers. Overall, it is not a bad park especially for the price, but it could use some clean up. - browncat
Oct 2016 - $35.00
We stayed here again, last time was November 2015. Located off I-35 but use KOA website directions to get to park. The park is located off access roads behind a RV dealership. Entrance roads are wide and concrete, as are the first 2 rows, the rest are packed gravel. Plenty of room to enter site and maneuver for big rigs. our site was concrete, very long but narrow and no patio. Mostly open, little shade! Small dog park, and it wasn't clean from previous dog owners, plus the dog park is located next to the park's sewer field. However, there is plenty of open grass areas around park to walk the dogs. Close to Sanger and Denton, plenty of places to eat and/or shop. This is a basic RV park, good for a short stay if visiting area. - KKColorado
Jul 2016 - $40.00
Nice quiet park with level concrete pull thru's that are long enough for any size rig. I had made reservations in advance since I was going to arrive after the office closed. When I arrived, someone had taken the site I was assigned. The manager was easy to get on the phone, and he quickly assigned me another site. The only negative I had was the sewer hookup was up out of the ground almost at the level of my RV hookup which made dumping black and gray water difficult. - Msubulldawgnwaco
13037 I-35 Sanger, Texas 76266 (map) | (940) 458-5979
Sites: 98 | Latest Rate: $21.00 | 14 Reviews
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Aug 2009 - $21.00
This park has very dusty roads. The sites are long, however there are no shade trees. It is convenient to the Dallas/Ft Worth area. - wilpower
May 2009 - $17.00
We stopped here for one night and used our Passport America to get the $17 rate. The checkin was easy and we were given a long pull through as we were leaving in the morning and didn't want to unhitch the towed. Very satellite friendly, WiFi is available but not free. - Ned
Apr 2009 - $25.00
We stayed at this campground last September and it had CATV. That's gone now and many of the amenities such as newspaper boxes are gone. Upkeep of the roadways is lacking; there are lots of potholes and debris in the area. The place looks run down. Next time we'll try Lake Ray Roberts for a short stay through north Texas. - Happy G


text: The Takeaway Edit this Published by The Takeaway RecycleBank: A recycling startup program that keeps on giving Listen 3 min Queue Download this audio Want to embed this player? Use the code below. More info. <iframe frameborder="0" scrolling="no" height="130" width="100%" src="https://www.wnyc.org/widgets/ondemand_player/takeaway/#file=/audio/json/8802/&share=1"></iframe> Share on Facebook Share on Twitter Email a Friend Guest: Ron Gonen, President and CEO of RecycleBank, a startup that rewards curbside recycling


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AK Anne Klein Sport Beech Reptile Flat
Sorry, this item is sold out!
Looks like you've got more shoe shopping to do - have fun!
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Item # dsw12prod5430085
Hit the road with the Beech flat! This adorable flat from AK Anne Klein Sport has a driving shoe sole for a responsive, comfortable fit.
- Reptile embossed faux leather upper
- Decorative buckle
- Round toe
- Flexible driving shoe sole
- Imported
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By: RedRosie
Location: MD
October 04, 2014
Great shoes
"Love these shoes. This is my second pair. The first pair were my every day, walk all over the city, shoes. This pair was purchased for a wedding. Very comfortable and true to size. I have narrow feet, so they were a tad wide."
By: GAP620
Location: New Jersey
April 22, 2014
AK Anne Klein Beech Flat
"I was so excited to purchase these shoes in black two weeks ago because the fit was so great. After wearing them 3 times, the black patent toes pealed in strips and are now white toes. I own many pairs of AK flats and never had a problem before. The Beech shoes are poor quality. I am so disappointed AK!"
By: JS27
Location: Harrisburg, PA
April 01, 2014
Stylish Cap Toe Flat
"I like how this shoe goes with many types of outfits - it can be dressed up or down. The patent cap toe is a nice feature. This is a comfortable shoe. The only complaint I have is that the material on the front of the toe gets worn off easily from general wear. Overall, I like the shoe and think it's stylish."
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text: This story is just more of the same I said, you said, they said, he said, she said business that pretty well dominates our political discourse (or lack thereof) these days. The question that came to my mind after reading this was, What's the problem with the CIA sneaking around offing Al Queda members? Sounds like a good idea to me and would actually give the DNP some more cred (at least with me, personally) if they did, in fact, give the approval to explore an opperation like this in the months following 9-11. True or not, they're taking the percieved political high road and denying they were told about this.


text:
POSTED ON: 1.23Oil and Gas Industry in 2017
The Dahill Group is a professional oil and gas recruiting, executive search, and consulting firm with 18 years recruiting experience and 25 years in the oil patch. Our Headquarters are located in Denver, CO, but our services extend beyond Denver to Houston, TX and Wyoming. Our approach is to understand each client's specific business requirements and hiring needs to match the ideal candidate to the business operation and company culture.
Our expert oil and gas recruiters provide an array of hiring and consulting services for our clients. Our executive search team has the extensive recruiting experience and in-depth industry knowledge required for recruiting and attracting the talent you need.
Our oil and gas recruiters are experts in matching top talent with outstanding positions.» MEET THE DAHILL GROUP
The Dahill Group is team of expert Oil and Gas recruiters. Our Executive Search Firm is based in Denver, Colorado, but also provides a full range of recruitment and executive search services in Texas and Wyoming.
Colorado Oil & Gas Association
IPAA – Independent Petroleum Association of America
Rocky Mountain Mineral Law Foundation
Petroleum Association of Wyoming (PAW)
POSTED ON: 1.23Oil and Gas Industry in 2017
POSTED ON: 9.19Will O&G companies be ready for hiring demands as industry ramps-up?
POSTED ON: 7.7Brexit & The Global Oil Market
POSTED ON: 6.20Resilient U.S. Shale Producers Look Ahead
POSTED ON: 5.2CO Supreme Court Rules Fracking Bans Illegal
POSTED ON: 4.29CO Bill proposal would give communities green light to ban drilling
POSTED ON: 4.8Finally, some positive predictions on oil
I had a very good job as an in-house oil and gas litigator. I thought I was perfectly happy – and then Elizabeth called me. An opportunity came across her desk that she felt fit me like a glove. Elizabeth spruced up my resume and helped me create a compelling CV supplement. A few weeks later, I had a new job with a real opportunity for upward mobility, better pay, better benefits, and a terrific and collaborative work environment. Elizabeth is knowledgeable and entrenched in the oil and gas industry, because she has been serving it for years. She didn't just understand my job, she understood their business and what they needed. What sets The Dahill Group apart is that they are looking for your next, best job, even when you aren't...
- Cristin Bracken - Associate General Counsel - SM-Energy Denver CO 80202
2016 Rocky Mountain Oil & Gas Rewards Nomination
Recruitment Agency of the Year


text:
September 19, 2014
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Two days after the earthquake that struck Nepal, the Pleiades satellites, developed by CNES, have captured an image of the capital city, Kathmandu. The satellites were tasked...
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1 December, a year after the launch of Pléiades-1A, Pléiades-1B will join its twin satellite in orbit at an altitude of 694 km. The Soyuz launcher is set to lift off from the...
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The first of the 2 Pléiades satellites is set to be orbited on 16 December by a Soyuz launcher from the Guiana Space Centre, ushering in a new generation of Earth imagery to add...
December 7, 2011


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KR fanatic, Veevz Kay recently shared with us on Facebook her favorite platform for customizing, her fingernails. Showing off her ambidextrous skills, Veevz adorned her own nails with a couple multi-colored Kidrobot logos as well as a Fatcap, Dunny, Wee Ninja and Reach Bear.
The post Kidrobot Inspired Fingernail Painting appeared first on Kidrobot Blog.


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You are 51% Dirty Slut.. Hmm.
Their Analysis (Vertical line = Average)
They scored 51% on Sluttyness, higher than 39% of your peers.
All possible test results
You are anti-sexual. For some reason it has been ingrained into you to think that sexuality is wrong. I think you just need to lighten up and get laid. Read more
Somewhat Sexual
You are somewhat sexual, but think that there are some things that just should never be done. Everyone is entitled to their own opinion, but please don't poorly judge others just because they enjoy li... Read more
Average Slut
You are a little slutty, but that's okay. You could have gotten a much higher score. Keep working at it, and maybe some day you will be as cool as me! Please rate my test if you enjoyed it! Read more
Dirty Slut!
You qualify as a dirty slut. You know that sex was meant to be enjoyed and you sure love it! You do, however, have some boundaries, which is a good thing. Go you! You are my kinda person! Please rate... Read more
Nasty Slut!!
Wow, I didn't think anyone would get this high of a score. You have a very dirty mind and have done some really dirty things. I applaud you for your openness towards sex and love for trying new things... Read more
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London King's Cross to Peterborough
Great British Railway Journeys, Series 4 Episode 9 of 25
Michael Portillo takes to the tracks with his copy of Bradshaw's Handbook, he travels the length and breadth of the British Isles to see what of Bradshaw's world remains. Michael is travelling port to port, from the centuries old naval hub of Portsmouth to the historic Grimsby docks. He discovers how derelict Victorian London is being rejuvenated, puts in a shift at a Cambridgeshire brick factory and meets the immigrant community built from it.
Last on
Thu 10 Sep 2015 16:45
|Michael Portillo
|Series Producer
|Michelle Heeley
|Cassie Farrell
|Executive Producer
|John Comerford


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4,056 total episodes
Interesting Naruto favourites but yea my ones with Bleach are different to most because i only started watching Bleach a month ago and im up to date now but i really like the whole Sagin being betrated by Touzen so there my favourite characters and i cant wait for the fight they must have soon in the manga, i really hope they do =p
Yea The picture is the Rock Lee Vs Garaa fight, Naruto was my first anime and Rock Lee will always be my favourite character of all time lol =p, i even have all the Naruto games and everything haha, who do you like from Naruto? and im from England =p
Hi, i see me and you setup on the site on the same day lol, i saw on the feed thing u have some anime in common with me and your profile is quite interesting but i havent watched as many anime as you have yet, have you been watching it for a long time and where are you from? =)


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China Has Done A Great Job This Year During G20 Presidency - Güven Sak - TEPAV Managing Director
30 September 2016
2016 T20 China Trade Panel Briefing - Sait Akman - Center Director TEPAV G20 Studies Center[More]
30 July 2016
Keywords: Finance Institute
The Global Financial Transformation from Asia to Europe Was Discussed TEPAV addressed the financial transformation throughout the world via country examples at a conference titled "From Asia to Europe: Global Financial Restructuring."
19/10/2011 ISTANBUL – TEPAV held a conference titled "From Asia to Europe: Global Financial Restructuring" on Wednesday, October 19, 2011 in cooperation with the World Bank. During the conference, speakers shared country examples about the financial re [More]
TEPAV Monitors International Financial Developments TEPAV Finance Institute will be directed by Fatih Özatay, professor of economics and former Deputy Governor of the Central Bank of Turkey.
24/07/2011 ANKARA - TEPAV will monitor international developments in finance and provide key information to the Turkish financial industry on the global developments. The Finance Institute established with this aim will be directed by Fatih Özatay, professor [More]
Sayfalar: 1
Trump's NATO question is a fair one
Güven Sak, PhD
19 February 2017


text: What is "space roar"?Esther Inglis-Arkell2/26/13 11:40am1073EditPromoteGo to permalinkIn space, no one can hear you scream. Probably because space itself can scream louder than you can. In 2006, NASA found something called "space roar" — it's a sound that is six times louder than anything that anyone was expecting, and it's clearly covering something up.


text: By NB Staff | April 9, 2007 | 11:34 AM EDT Share it Tweet it 0 shares Discuss the news of the day and anything else here... Open Thread NB StaffBio | ArchiveNB StaffMore from NB Staff Like this article? Get more like this in your inbox for free! Thank you for subscribing!


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Watch Featured Movies
Million Dollar Baby: A hardened trainer/manager works with a determined woman in her attempt to establish herself as a boxer.
|IMDB: 8.1/10 Metascore: 86% RT: 91%
|January 28, 2005
|132 mins
|Drama Sport
|Clint Eastwood
|Anthony Mackie Benito Martinez Brían F O'Byrne Brian Finney Bruce MacVittie Christopher Gilbertson Clint Eastwood David Powledge Hilary Swank Jamison Yang Jason Williams Jay Baruchel Jim Cantafio Joe D'Angerio Jude Ciccolella Kim Strauss Kimberly Estrada Kirsten Berman Lo Ming Lucia Rijker Marc De'Antone Marco Rodríguez Margo Martindale Mark Thomason McKay Stewart Michael Bentt Michael Peña Miguel Perez Morgan Freeman Ned Eisenberg Nina Avetisova Riki Lindhome Robert McMurrer Sean LoGrasso Sean O'Kane Spice Williams-Crosby Steven M Porter Susan Krebs Ted Grossman Tom McCleister VJ Foster
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|Posted by Goopus 1 year, 2 months ago
This movie builds you up so far, makes you believe everything's going to be okay, makes you want to believe it's going to be okay even if you know how it's going to end. The characters are rich and real and well-rounded, so well-acted.
|Posted by heidi13 5 years ago
Really, truly one of the best movies I've watched. It breaks your heart open and leaves you in an emotional mess for days afterward. It may hurt, but every second of this movie is astounding.
|Posted by patten11b 6 years, 10 months ago
movie is definetly top 10 EASTWOOD fliks a must watch and Hillary Swank gives a top notch performance 9.5/10
|Posted by redhead60 7 years ago
Extraordinary movie ,Compelling for the tenacious young woman,to train to become the Best boxer there is...
|Posted by da_conz 7 years ago
A moving story - well deserved Oscar for Hilary Swank.
|Posted by dramucom 6 months ago
This film leaves you emotionally drained and sits with you a long time after.
|Posted by whywouldyou 1 year, 10 months ago ( Edited 1 year, 10 months ago )
I am a long time Clint Eastwood as an actor fan. This was the first movie I seen of his as a Director. Now I have a love hate relationship with him being a Director. I love the movie's he wrote or Directed. I hate the way they all end and I mean hate them. They never ever go the way you would expect it to. I brought the Dvd of Million Dollar Baby and was so excited to see it since everyone I know said how great of a movie it was. I got to the ending and it made me so upset that I broke the Dvd and swore against watching another Clint Eastwood movie. Then Gran Torino came out and I just couldn't resist watching it. Lets just say I broke that Dvd too. The sad part was that it was a rental.
|Posted by naturrel 3 years, 8 months ago
Great film. One of my favorites. Great story, inspiring, emotional and quite deep. Clint Eastwood is wonderful, I love the themes he touches on and portrays in his films/movies. Morgan Freeman, Hilary Swank, they all did wonderful, yeah I really loved and love this film. 10/10
|Posted by kproyutbe 3 years, 9 months ago
i have seen this movie in my movie case and constantly ignored it......one of the worst decisions i have ever made
|Posted by robchar 4 years, 2 months ago
Clint Eastwood, Morgan Freeman, and Hilary Swank, an amazing cast, with an amazing story, and this was an extremely amazing movie, but the ending is very sad I usually don't do this but this movie deserves this type of a rating, I give it a 10/5. That's right a ten out of five, might not make sense, I usually rate movies from 0 to a 5, however there are some this extremely amazing movies that deserve to go higher and this is one of them.
|Posted by ZombieCassandra 4 years, 4 months ago
Good movie. Inspiring in a way. Made me cry a little bit at one point. The acting was brilliant. Glad I finally watched this.
|Posted by Just_A_Puppet 4 years, 5 months ago
Found this film quite disturbing when I first watched it, as I was 12, and had never been exposed to anything of the like! Though now, after re-visiting it find is to be an astounding film, albeit very sad. If you intend to watch it, I'd say go ahead, but have the tissues at the ready!
|Posted by gemcat19 4 years, 7 months ago
Wow. This whole film is captivating from begining to end. I laughed, cheered and cried! The actors are all briliant and the plot is faultless. 5/5 and one of those films that everyone should watch at least once.
|Posted by Scene68 4 years, 11 months ago
Great movie. Sad ending. Great performances from all Clint Eastwood, Hilary Swank and Morgan Freeman it shows why Hilary Swank won her Oscar for this film. 10/10
|Posted by Dscaggs 4 years, 11 months ago
Any movie with Morgan Freeman doing voice over is excellent. 9/10.
|Posted by Sille 5 years ago
Very inspiring :) 10/10
|Posted by balverio 5 years ago
very sad movie, and well done - 10/10!
|Posted by emer_h 5 years, 1 month ago
Liked this film, Clint Eastwood and Hilary Swank were brilliant, definitely worth a watch.
|Posted by lindsaylunacy 5 years, 3 months ago
I really enjoyed this film. The ending was unexpected. It was a nice, yet very sad change. I've been wanting to watch this movie since it came out, I wish I didn't wait this long because I really liked it. It had me captivated from beginning to end. The acting and characters were great. I would recommend this film to people for sure.
|Posted by raidmar 5 years, 3 months ago ( Edited 5 years, 2 months ago )
great movie.i love sports and love Eastwood's works,put them together and it's a great combo.10/10
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text:
|Angels Camp, California
|Former Occupation
|Owns a construction business
|Years Pro
|Top 10 Finishes (Wins)*
|6 (3)
|Career Earnings*
|Last 3 Finishes
54 - Lake Ouachita, 8/11/2011
58 - Lake Lanier, 8/5/2010
34 - Table Rock Lake, 3/31/2005
|Angling Hero
|Dave Gliebe - "When I was a kid in California, he won all the tournaments and took flipping back east. He was the guy." Rick Clunn -- "He's so good it's unbelievable."
|Favorite Lake
|Clear Lake (Calif.) - "You can do everything there and catch giant bass."
|Least Favorite Lake
|Lake Toho (Fla.) - "I'm oriented more towards the bank. I need a line to follow. At Toho the grass grows clear across the center of the lake."
|Favorite Technique
|Bass Cat
|Favorite Food
|Favorite Music
|'80s rock 'n' roll (Van Halen)
|Favorite Book/Movie
|Of Mice and Men (book)/Forrest Gump (movie)
|When Not Fishing
|"I love to hunt deer and quail."
|Why He Fishes
|"I was brought up fishing with my dad and I'm a really competitive person. I probably wouldn't fish as much if it wasn't for tournaments. And I love the outdoors."
|Click a column heading to re-sort.
|Tournament Finishes
|(Only recent tournaments are included. Not necessarily a career listing.)
*BASS events (all events because BASS does not report Tour and Open earnings separately) and FLW Tour.
Biographical data last updated: 7/16/2002
You may report any errors or omissions by clicking here. Please be certain of your information before submitting anything. Submissions will be examined, and if approved will be included in a future update.
By Todd Ceisner BassFan Editor For his money, Todd Castledine says the current crop of bass anglers he competes against regularly in southeast Texas
By John Johnson BassFan Senior Editor Tim Horton's victory at last week's Lake Okeechobee Bassmaster Elite Series was akin to a race-car driver who
By BassFan Staff It looked like it was going to be easy, but it turned out that it wasn't. Nonetheless, Tim Horton's long dry spell is over. The


text:
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it is a walk to try and keep your arse cheeks apart while suffering from arse rash also caused by anal penetration
Dude that blokes got bow leg i bet hes suffering from arse rash
by Robj2502 June 24, 2007
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Cognitive Teachable Agent in Virtual Learning Environment With Fuzzy Cognitive Map
Today more and more learning technologies are developed in the virtual environment with intelligent agents to promote students' learning experience and efficiency. Teachable agent is a kind of pedagogical agent that promotes the students' learning through teaching it with concept maps e.g. Betty's Brain. However, the knowledge representation with concept map may be insufficient for a complex knowledge structure, and there will be a limited number of quizzes derived from the concept map. As a result, the teachable agent is taught with limited knowledge.
Provided by: Nanyang Technological University Topic: Developer Date Added: Feb 2011 Format: PDF


text:
10 Item(s)
- Great lace table runner!Review by Tia
Quality Price Fit Appearance
You cannot beat these ivory lace table runners. I bought them to go over the 120 inch pink and ivory tablecloths. I wanted a vintage feel for my wedding, and these were perfect. The quality was great, and the price was even better. Note, however, that the color is a tiny bit lighter than it appears in the photo above.(Posted on 3/21/16)
- Awesome for the price!Review by Tia
Quality Price Fit Appearance
Love these table runners. They look great on our vintage pink tablecloths.(Posted on 8/25/15)
- Looked good!Review by Natalie
Quality Price Fit Appearance
I just planned a 1940's style (simple, elegant, classic) event. And used these as the table runners on a white tablecloth! They looked great. I had one tassel get pulled off, but I tied it back on with doily crocheting yarn, and you couldn't even tell! Love these!(Posted on 8/11/15)
- Perfect for our needsReview by Roberta
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I am helping my future daughter in law get ready for a vintage inspired wedding and these are perfect!(Posted on 7/8/15)
- Beautiful lace!Review by Megan
So glad I purchased this, you can't beat this low price! Perfect addition for that romantic/vintage feel. Using these at my upcoming wedding for dessert/card tables.(Posted on 2/2/14)
10 Item(s)


text:
© Copyright 2017 redsoxocd. All rights reserved.
Status: Finished
Genre: Editorial and Opinion
I stumbled upon this and decided to give it a go....
I don't think ANYTHING should be handed out at schools without parental consent. This goes for condoms, oral contraceptives, whatever!
It's sad that a school had that many pregnancies in one school year. The problem is not just the schools, though.
I think it is up to the parents AND school officials to work TOGETHER with our kids. Unfortunately, this does not usually happen.
I am a proud mother of two beautiful girls, ages 10 and 6. My older one is learning about some changes going on with her body, and I have told her all she needs to know, with her appropriate age level.
When she reaches high school, I know there will be a lot going on there. ( I actually remember high school) I don't want them giving anything to that nature to my child. At all.
You brought up a good point. It's one thing to hand out condoms, but to give out oral contraceptives....Do you know how many women can be allergic to them? I'm not against birth control pills, but it should be handed out by a DOCTOR.
Anyway, your article brought up some very good points, and I just wanted to add to it.
thanks for your comment. A few weeks after this incident happened it surfaced that many of the girls were part of a "pregnancy pact". They were all trying to get pregnant around the same time so that they could be single mothers and raise thier children togther. Some of the girls even paid guys to sleep with them, in one case it was even a homeless guy.
So your right, this is definately something that has to do with both education and, in this case, especially at home.
Short Story / Romance
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Welcome to WarthPhoto.com, I invite you to look around and browse the site. As a freelance commercial and editorial photographer I love to collaborate with others and create images for advertising, promotional and editorial use.
Please call 740-703-2396 or email me to discuss your photography needs.
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That's great news. Thanks for posting.
We had issue with our 2 year old Samsung LCD 40inch TV. The TV will take 30-45min to start. After the start we were getting only the voice and not the image. I read several forums and concluded it is a faulty capacitor problem. Called Samsung customer support. They seem to understand the problem is happening widely for other Samsung TV's as well. They send me a technician with in a week to fix the bad capacitor and the problem is solved. I was planning to do this myself thinking Samsung will not honour this after warranty. But they took care of it with no issues. Thanks Samsung and quick support.


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2015 is the year of the Twelve Month Artful Pledge and it's time for September's focus: Listen. September: Listen It's a great month for listening. The Downtown Guelph Businesses Association continues their run of great noon-hour concerts which are offered every Thursday afternoon at 12. These free and family friendly concerts feature local musicians and are a great way to… Read more →
Category: Art
Twelve Month Artful Pledge – August: Share
2015 is the year of the Twelve Month Artful Pledge and it's time for August's focus: Share. Do you have a favorite artist, musician, maker, event, or artisan? August is the month for sharing! I can definitely do that. In fact I do indeed do that every day over on bohemianizm. Check it out! Read more →
Twelve Month Artful Pledge – July: Attend
2015 is the year of the Twelve Month Artful Pledge and it's time for July's focus: Attend. July is a big month for Guelph as Hillside Festival rolls into town. It's known as one of the greatest festivals around. This award-winning and highly acclaimed festival boasts a great format and many amazing programs, which really celebrate the spirit of its… Read more →
Twelve Month Artful Pledge – June: Watch
2015 is the year of the Twelve Month Artful Pledge and it's time for June's focus: Watch. June is a month that is full of great things to watch. …This kind of engagement in your local arts scene provides both entertainment and excitement while also contributing to the local investment and the economy in our city. …Beyond the local investment… Read more →
Big Bang Kiss, Girls Love Skulls // Lora Zombie
Big bang kiss, creator of the Universe. Punks in outer space. Falling angel, out of gravity. Zombie love eating the moon. Magic garden, adventure time. Space panda, follow the unicorn to kiss a unicorn. Love shock will come to take sadness away. ……….Sleep. ……….Scream. ……….Whatever. Choose your weapon, bomb rider. ……….The protector. ……….The great escape. ……….War games. ……….Tank and a girl. … Read more →


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Hey, watch Kaze No Stigma, it has a bit of romance but no kissing :D
The fighting is good and has good comedy so give it a try!
Welcome to Anime Planet !
anyway, you know who this is :)


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No Place To Go (You Gonna Wreck My Life)
Features of This Trackclassic soul qualities
blues roots
acoustic rhythm piano
extensive vamping
major key tonality
a dynamic male vocalist
a prominent harmonica part
a mid-tempo swing feel
and many other similarities identified in the Music Genome Project
These are just a few of the hundreds of attributes cataloged for this track by the Music Genome Project.show more
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text:
Driven by money, power and success, Gabriel has worked ruthlessly to get to the very top of the banking game. He's not going to let the inconvenience of a terminal brain tumour get in his way. But the tumour has other ideas. As it grows, it appears to be doing strange things to Gabriel's personality. Whether he likes it or not, he seems to be becoming less selfish, less mercenary, less unlikeable. Once he could dismiss the rest of humanity as irrelevant. Now he's not so sure.
Women in particular are becoming worryingly three-dimensional. And none more so than Caitlin, the unremarkable girl he sees busking on the steps of St Paul's Cathedral. When she plays her violin, Gabriel could almost believe that he has a soul.... But as each day that passes brings him closer to his last, has time run out for second chances?
Best-selling author Gavin Extence pushes the envelope again with another thought-provoking and funny novel about the surprises, good and bad, that life can throw at us.
©2016 Gavin Extence (P)2016 Hodder & Stoughton
"A brilliant author"
The story is good, deep, meaningful and thought provoking. The setting is modern bringing to the fore events which happened during the financial crash whilst touching upon subjects which affect us all.
A book which leaves you thinking long after it's been read.
The narration is also excellent.
"Absolutely Brilliant"
15 out of 10 if I could. Equally as good as the author's 1st book. Interesting story lines merged into one. Poignant & again made me audibly laugh quite a bit, even though there was tragedy. I hope Extence is writing a 4th book by now. I will be buying all of this author's works as soon as they are released. For me I found His 1st book was exceedingly good; it was excellent. His 2nd book very good & for me this 3rd book was again excellent. Hope he uses the same narrator for his 4th. I love the narrator's delivery too.
"Enjoyable story !"
A good and in spite of the subject funny story. Well worth of a credit.
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text:
Finally got the acoustipak lite! I've made macro comparison shots: http://www.imagebam.com/gallery/tfurpsnpf3r5452tbst0juwbav7n3gjs/
The biggest patch is the acoustipak, the smallest is the old Akasa(salvaged from a used PC) that's currently in my T50RP and the one in between is the Akasa that's currently for sale. It seems pretty clear that the old Akasa has bigger cells, then the new one's are smaller and by far the smallest ones are the AcoustiPak. They don't even have the same texture, both Akasa feel more "plasticky" than "foamy". This really looks like the real deal to me! I believe my old Akasa is the one every review on the web found useless for its original purpose(attenuate fans whining).
I will use newplast on the baffles and its surrounding seal, and I believe I will make some sort of "pyramid" going up to the height of the driver...then fill it w/ screwdrivers holes of course, remove the side vents, solder the cable directly onto the drivers, ditch the front driver felt, use my own felt instead and slap sa5k pads on it...et voilà
Edited by leeperry - 11/22/11 at 10:25am


text: PEI Ground Search & Rescue Posted on December 24, 2015 by garsenault PEI Search and Rescue founded in 1995. We are on call 24/7 and go out to search in any weather conditions We train 10 months a year to maintain our skill sets. We have a partnership with Project Lifesaver PEI. We are specially trained to search for people that have disorders that make them more susceptible to wandering We supports other non-profit organizations we support and Promote the Adventure Smart Program