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- "Art rediscovers, generation by generation, what is necessary to humanness. " -John Gardner
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Tag Archives: Christmas
Christmas trees and car wrecks.
I look forward to decorating our home for Christmas all year long. It isn't that we have lavish decorations. Those things require money and storage space, with the latter being perhaps the greatest of all constraints. We stick to one … Continue reading
Posted in Uncategorized Tagged Christmas 17 Comments
Weary Joy.
I know you are so tired of hearing about our Christmas card by now, and even more tired of me moaning and groaning about this year. But, at the eleventh hour, and after a slight altercation at the post office … Continue reading
Posted in Uncategorized Tagged Christmas 7 Comments
2014 Christmas Card Out-takes
It's that time of year again… CHRISTMAS CARD SEASON! My fridge is getting all gussied up with beaming faces. Our cards will hopefully arrive here in time to be shipped out before we leave for Christmas with our families. The … Continue reading
Posted in Uncategorized Tagged Christmas, Holidays 6 Comments
A Christmas Walk
Last night James and I did one of our favorite low-key Christmas traditions, walking across Capitol Hill to see some of our favorite holiday decorations. We started at Starbucks, loading up on those amazing sugary holiday drinks before wandering over … Continue reading
Posted in Uncategorized Tagged Capitol Hill, Christmas, Holidays, iphone pics 8 Comments
The Best Christmas Party
Usually, we throw some sort of Christmas party, some sort of Yuletide gathering where we eat too much delicious seasonal food and enjoy time with friends during this busy season. Last year we hit on the genius idea to skip … Continue reading
Posted in Uncategorized Tagged Christmas, college, party 7 Comments
The most wonderful time of the year
It seems like every year I get more and more excited about the Christmas season. That stretch between Thanksgiving and Christmas is just packed with so many wonderful things. The expectation of Advent, visits to family, all the best songs, … Continue reading
Posted in Uncategorized Tagged Christmas, DC, Holidays 13 Comments
Merry Christmas!
Merry Christmas y'all! After all your advice and input about our Christmas cards, we sent out these a little while back. We did one of those update letters (or the "humble brag" according to James) on the back, superimposed over … Continue reading
Posted in Uncategorized Tagged Christmas, Holidays, photography 5 Comments


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Dilled Cucumber and Tomato Salad
Watermelon Cucumber Feta Salad
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Here's a delicious cucumber and bell pepper salad served with grilled chicken or fish – a delightful side dish.
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Skip to comments.Manatees mating in local waters (Hugh Manatee Alert)
Posted on 06/14/2008 5:56:16 PM PDT by abb
Along the Emerald Coast, it's fairly common to see dolphins, manta rays, sea turtles and other marine animals. But manatees cause more of a stir.
That's why Niceville veterinarian Jenny Fortune and her husband were slow to identify what was swimming between them in hip-deep water near Fred Gannon Rocky Bayou State Park recently.
"I'm looking at it, going Oh my God, what is it?' " Fortune said. "These things are giant. Your brain just doesn't compute."
She said they first thought it might be a large manta ray. They quickly realized that the creature wasn't flat, but very, very bulky.
"It just kept going, it was like a train," she said. "Finally, at the tail end, you could see a flipper."
Manatees do visit local waters infrequently during the summer, according to Andy Garrett, a biologist with the Florida Fish and Wildlife Conservation Commission.
"It will be hit or miss," he said. "It won't be like traveling the waterways around South Florida."
They even mate here, he said.
Fortune, who called the FWC wildlife hotline after her manatee sighting, said she was told that there were five breeding manatees near Destin.
No one at FWC could confirm that conversation. However, officials said that folks who do see courtships often call it in out of concern for the animals.
Garrett said that several males will chase a lone female into shallow water, where she will wait until she is ready to receive them.
Typical calls involve folks worrying that the female is injured and other manatees are trying to help it by pushing it to shallow water.
"That's what they think they're seeing," Garrett said. "What they're actually seeing is the male trying to get underwater to mate with her and she's not letting them."
Daily News Staff Writer Wendy Victora can be reached at 863-1111, Ext. 1478.
Have you seen a manatee in local waters? Call Wendy Victora at 863-1111, Ext. 1478, or e-mail her at firstname.lastname@example.com. The Daily News is also interested in publishing your photos or videos of manatees found in the area.
Hey, folks, your father ever tell you he was NOT hurting your mother???
Don't care about manatees. I stopped watching Family Guy a long time ago.
One of the saddest things I have ever seen was a huge manatee swimming around and around in a small shallow pool in a town on the west coast of Florida. I am not a big fan of keeping wild animals in captivity and that includes zoos.
LOL the 9 yr. old came running into the house last week to tell us one turtle was trying to hurt another turtle. Her 8 yr. old cousin had to tell her they were making babys. Boy was she disgusted.
Liberals get to hyperventilating over some of the weirdest things. I guess watching two walruses copulate would turn some people on. I'm not one of them.
Good one, abb!
I actually did sit here and LOL, for several minutes.
And I'll probably wake up laughing some time late tonight!
2 gray whales fonicating in 6 feet of water has got to be funnier.
We watched 2 of them getting it on in Magdalena Bay about 30 years ago and about died laughing!
We've not had a hugh manatee alert in a couple of years. About time for one, doncha think?
Hugh Manatee Alert!
Next we'll be talking about "Mooses" and "Cheese," fer Pete's Sake!
He has that manatee face.
It was only a matter of time that a legit article would come across that we could legitimately use the Hugh Manatee!
Sorry... wasn't paying attention. My wife walked into the room and I had to show her the Hugh Manatee! picture, then forgot where I was.
"They quickly realized that the creature wasn't flat, but very, very bulky."
As I type this reply, I "quickly realize" that I am sitting on front of a computer and not a skid-steer loader...
It's been a couple of years since we've had a Hugh Manatee alert. We FReepers have been remiss in keeping up with Manatee news. Just look here!
Manatee sexual predator arrested for failing to register
And more.
COLLIER COUNTY: It was an impressive sight Friday in Collier County as crews helped an injured 1,600 pound manatee and her young calf back into the wild
Storms damage Manatee homes
Back in '92, I took my scouts snorkeling on the Seawanee river where threre were reports of Manatee sightings.
One of the older scouts raised the hue and cry Manatee, manatee, manatee!!...
Then after attracting all the attention of those nearby.....never mind, it's just Bert but it sure looked big enough
Several Manatee projects avoid veto pen
Manatee Sheriff's deputies' salaries frozen
Apparently, this Manatee sexual predator is named Hirum. A brother of Hugh, perhaps?
Manatee Middle Schools summer tennis camp
"Is anyone here a marine biologist!!"
Some updates this morning.
Cupid's employee convicted in Manatee prostitution sting
Manatee land market said to be iffy
Elderly Man Missing In Manatee
Foreclosures create ripple effect in Manatee
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


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Service offshoring and productivity in Western Europe
Using comparable data for nine Western European countries, this paper finds that service offshoring exerts positive and economically large effects on domestic productivity. A one percentage point increase in service offshoring is found to raise Total Factor Productivity by 0.5-0.6 percent.
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Volume (Year): 6 (2008)
Issue (Month): 35 ()
Pages: 1-8
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The last major advance in the treatment of metastatic bladder cancer (BC) took place in 1997 with the advent of gemcitabine. Despite this advance, visceral metastases are usually fatal. The overall goal of the proposed studies is to develop small molecule inhibitors that block a critical node in the metastatic process. We found that Rai GTPases serve as the molecular switches of a therapeutically tractable signaling pathway that allows UC cells to grow in the lung, the most common visceral metastatic site. The clinical significance of this pathway and validity of Rai as a therapeutic target is supported by finding that high Rai expression in tumors places patients at higher risk for metastasis and the requirement of Rai expression for lung metastasis to occur in animal models of UC. Our Guiding Hypothesis for this application is that small molecules targeting Rai provide effective therapy for metastatic UC. With support from the MD Anderson Bladder SPORE Developmental Research Program (DRP), we evaluated >500K compounds for their ability to bind RalA or RalB in computational and combinatorial screens and selected 99 "hits". These were evaluated in a series of secondary assays allowing us to select Rai Binding Compound (RUC)8 and 10 to be pursued in this application. RUC8 and 10 were selected because they: 1) inhibit RalA to RalBPI binding in human UC cells and RalA induced spreading in murine embryo fibroblasts;2) inhibit in vitro monolayer growth (IC50 0.5-1.9 pM) of human UC cells;3) bind RalB directly by nuclear magnetic resonance (NMR) spectroscopy;and 4) have good pharmacokinetic (PK) properties in mice (Cmax 1.3-23 pM, T1/2 3.7-4.6 hrs). To develop this novel class of agents we propose the following Specific Aims:
Aim 1 : Characterize higher potency 2nd generation compounds based on RUC8 and 10 using medicinal chemistry, computational fragment-based design, and similarity search of chemical databases. In the unlikely situation that higher potency compounds are not found in Aim 1, we will pursue Aim 2 and 3 using RUC8 and 10, given their adequate IC50 and in vivo PK.
Aim 2 : Evaluate 2nd generation compounds for their in vivo therapeutic efficacy in novel human UC models of visceral metastasis.
Aim 3 : Develop predictive biomarkers of response to antlRal therapeutics in human tissues that will position us for Phase 1 trials by end of this project. Documented interest by Astra Zeneca in our work improves overall chances for success in translating our novel Rai inhibitors into the clinical setting as anticancer therapeutics.
Inhibition of GTPases and its translation into viable cancer therapeutics has been unsuccessfully sought since discovery of Ras in 1982. Our preliminary work demonstrates first in class inhibitors of the Rai GTPase which may constitute the first significant therapeutic advance in metastatic bladder cancer since 1997. Since Rai GTPases are also important drivers of human pancreatic and prostate cancer growth and metastasis, this work has the potential to provide an effective therapeutic for several major cancer types.
|Choi, Woonyoung; Porten, Sima; Kim, Seungchan et al. (2014) Identification of distinct basal and luminal subtypes of muscle-invasive bladder cancer with different sensitivities to frontline chemotherapy. Cancer Cell 25:152-65
|Hoang, Anthony N; Agarwal, Piyush K; Walton-Diaz, Annerleim et al. (2014) Clinical significance of ureteric 'skip lesions' at the time of radical cystectomy: the M.D. Anderson experience and literature review. BJU Int 113:E28-33
|Benedict, W F; Fisher, M; Zhang, X-Q et al. (2014) Use of monitoring levels of soluble forms of cytokeratin 18 in the urine of patients with superficial bladder cancer following intravesical Ad-IFN?/Syn3 treatment in a phase l study. Cancer Gene Ther 21:91-4
|Figueroa, Jonine D; Ye, Yuanqing; Siddiq, Afshan et al. (2014) Genome-wide association study identifies multiple loci associated with bladder cancer risk. Hum Mol Genet 23:1387-98
|Culp, Stephen H; Dickstein, Rian J; Grossman, H Barton et al. (2014) Refining patient selection for neoadjuvant chemotherapy before radical cystectomy. J Urol 191:40-7
|Chakravarti, Deepavali; Su, Xiaohua; Cho, Min Soon et al. (2014) Induced multipotency in adult keratinocytes through down-regulation of ?Np63 or DGCR8. Proc Natl Acad Sci U S A 111:E572-81
|Cancer Genome Atlas Research Network (2014) Comprehensive molecular characterization of urothelial bladder carcinoma. Nature 507:315-22
|Dinney, Colin P N; Hansel, Donna; McConkey, David et al. (2014) Novel neoadjuvant therapy paradigms for bladder cancer: results from the National Cancer Center Institute Forum. Urol Oncol 32:1108-15
|Yan, Chao; Liu, Degang; Li, Liwei et al. (2014) Discovery and characterization of small molecules that target the GTPase Ral. Nature 515:443-7
|Lee, Eugene K; Ye, Yuanquing; Kamat, Ashish M et al. (2013) Genetic variations in regulator of G-protein signaling (RGS) confer risk of bladder cancer. Cancer 119:1643-51
Showing the most recent 10 out of 178 publications


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Cutaneous Water Acquisition by the Thorny Devil (Moloch horridus: Agamidae)
Philip Withers
Journal of Herpetology
Vol. 27, No. 3 (Sep., 1993), pp. 265-270
Published by: Society for the Study of Amphibians and Reptiles
Stable URL: http://www.jstor.org/stable/1565146
Page Count: 6
Topics: Skin, Surface water, Lizards, Water loss, Puddles, Capillary action, Film water, Rain, Water uptake, Moisture content
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The skin of the thorny devil readily absorbs water, like "blotting-paper." The volume of water held in the cutaneous capillary system is about 3.7% of the body mass. Water is conveyed to the mouth by the cutaneous capillary system, where it is imbibed. The low permeability of the skin to evaporative water loss and lack of dyed water absorption suggest that there is not any transcutaneous water absorption across the skin itself. Interscalar channels about 5-50 μm wide, and an overlapping shelf around the edge of the scales that forms a sub-scalar channel, appear to be the primary cutaneous surface features that are responsible for the "blotting-paper" action of the skin. The capillarity force that absorbs water to the skin supports a pressure head of about 10 cm water. This capillary head is not consistent with the inter-scalar capillarity channel dimension of about 5-50 μm, but to about 220 μm. One ecological role of the "blotting-paper" skin of the thorny devil is clearly the direct uptake of rain that falls on the skin or from puddles. In addition, the cutaneous capillary system of the thorny devil enables water absorption from moist sand.
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Journal of Herpetology © 1993 Society for the Study of Amphibians and Reptiles


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Milwaukee 11" Extension
The 11 inch extensions can be coupled between the Shank and the Turbo Tunnel bit for extra deep hole drilling with Milwaukee Turbo Tunnel Bits.
- Extension with stud
- 11 In. length
- Interchangeable drilling heads
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In The Box
- One (1) 11" Extension


text: Paraguayan Bus Drivers Crucify Themselves To Try To Get Jobs BackMichael Ballaban8/31/13 10:00amFiled to: buslopnikjobsparaguaylabor negotiations684EditPromoteShare to KinjaToggle Conversation toolsGo to permalinkEight bus drivers in the South American nation of Paraguay have crucified themselves after being fired two months ago. They say they were let go after demanding better pay and conditions, and have remained nailed to their crosses for at least two weeks. We've heard of tough labor negotiations before, but this one may just be the most extreme. The protesting men in the city of Luque have have also been on a hunger strike since literally having driven nails through their hands and index fingers into the wood behind them, according to CNN: Juan Villalba is one of the crucified bus drivers. Villalba is the secretary of the Paraguayan Federation of Transportation Workers. He told Paraguayan media that his group is willing to take the protest "to the very end," regardless of the consequences. His wife, María Concepción Candia, also nailed herself to a wooden cross Wednesday to show support.The driver's wives are taking turns being crucified in one-day rotations, according to the Telegraph. The crucifixions are part of a larger labor protest by about 50 bus drivers, though only the eight and their wives have gone to such extreme measures. Advertisement Advertisement The bus company, Vanguardia, has so far offered five of the drivers their jobs back but the men say they will not give up their protest until all eight are re-hired. I'm not a medical expert, though I can't imagine having nails driven through one's hands is very conducive to things that occur in the normal operation of a bus, such as turning a key, or gripping a wheel, or waving at passing motorists.It's a little dramatic, I suppose, but it gets the message across. What message that is, is open to interpretation. Feel free to debate it in the comments below.H/t to Creative AccidentsReply68 repliesLeave a reply


text:
Closeouts. Columbia Sportswear's Crushed Out snow pants are fully insulated performers that keep her warm, dry and looking great on the next snow day.
Do you own this product? Write a review and help others with their buying decisions!Write a review
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WordPress e-Commerce Website (adult products)
- Status Closed
- Budget N/A
- Total Bids 34
Project Description
Firstly, please note the products for our e-commerce website are adult products, so all bidders must be +21 years of age, and not be offended by such items.
We are looking for someone experienced in setting up a WordPress based e-commerce website and importing products thereto from a CSV file. You MUST have a lot of experience in setting up a WordPress based e-commerce website, and specifically, importing product from a CSV file.
Before we can accept any bid, you must link to the website of the theme you intend using for our project. We will then review its features, stability and functionality. We can then also advise you of what design changes and custom work we may or may not require.
We have come across several WordPress e-commerce themes that look awesome and are priced from $50 to $200 that would be suitable for our site. Do not include the price of buying the theme in your bid, as we can purchase it directly.
Basically all we need is a theme that you recommend for an adult e-commerce website; that you have a lot of experience working with, as you will customize it for us, importing our related products from a CSV file.
We require examples of WordPress e-commerce sites you have previously set up.
Without examples, we can not consider you for this project.
We also require you to be available to talk on the phone. We probably will not call, but if either of us need to speak to help the project along, this option must be available to us, and we will call you.
Our CSV file looks like this:
Product Code; Product Name; Page Title; Description; Meta Keywords; Meta Description; Price; Sale Price; Category; Secondary Categories; Brand; Status; Image URL; In Stock
Please note the "Image URL" is hosted on a 3rd party website, which must be saved to WordPress to generate the various sized thumbnail images.
We are experienced WordPress users, but have never worked with a WordPress e-commerce theme. Therefore please ask any and all questions so you are prepared to place an accurate bid and timeframe, so the project completes smoothly and successfully.
Funds will be deposited upfront into escrow and released when the project is 100% completed.
Thank youGet free quotes for a project like this
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Hoppy Holidays
Tis the season for winter brews
by Kay Arcuragi
From the December, 2013 issue
As winter settles in, local brewpubs are rolling out seasonal beers. Mike Maroney at Ann Arbor Brewing Company says that compared to the light and crisp fruit-flavored ales featured in the summertime, winter brews tend to be "bigger bodied, a little bit sweeter, high gravity [alcohol content], and a lot of malt character." ABC recently released its bestselling Terminator Dopplebock, which comes back each year by popular demand.
Each brewery has a set of "house beers" served year-round and a few rotating beers, which change every month or so. Wolverine State Brewery's will roll out a new Belgian-inspired dark beer, "Grand CruZ," around Christmas, joining its offiical seasonal brew, Winter Lager.
Other brews returning to local taps are Grizzly Peak's White Christmas White Ale in mid-December, and their Edwyn's Warm-up Ale in early January. Grizzly Peak's head brewer, Duncan Williams, says his predecessor developed White Christmas, and people have loved it ever since. This year, he's adding a new recipe of his own; if Three Ships Ale Belgian style Tripel proves popular, it may be back next year.
[Originally published in December, 2013.]
What's happening in local stores and eateries, and restaurant reviews.>> Blogs
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Fred Lalcaj takes over the original babo.
Table talk: February 2016
PTO Thrift Shop Expands
The Showcase gets its own space.
Avalon Café and Kitchen
Bread is only the beginning.
Return of the Independent Pharmacy
Ray Albaneh has a mentor and a plan.
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Owner Financing Homes in Coffey, MO
|6 Listings Found
Multi-Farm Auction in Harrison & Mercer County, MO Monday, November 26, 2012 at 10:00 AM Auction Location: Bethany Community Center,...
105 N 25th St, Bethany, MO, 64424Details »
farmer, and roughly 80 acres of land that is registered in two long-term CRP contracts. It's priced right
Martinsville, MO, 64467Details »
, tillable land and a creek that runs through the property. Currently there is approx 38 acres in row crop
Martinsville, MO, 64467Details »
3.6 Acre Property For Off Grid Living! $17,900.00 Owner Financing Terms: $180/Month with $1
Albany, MO, 64402Details »
for equipment large pole barn. Great for hunting deer and turkey. CRP contract brings $14 448/year.
Maysville, MO, 64469Details »
,300 a year and contract is up in 2017. You can enroll for more money or put into crops. There are 2 nice
Trenton, MO, 64683Details »
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The aim of the Pure biomass –project is to raise awareness about the technical and economic benefits of the use of different types of biomass in energy production in the Kurzeme region in Latvia and Southwest Finland. The project team will evaluate the biomass potential in the areas and estimate the cost for replacing fossil fuels. There will be also pilot projects about local biomass and their technical-economic aspects.
Pure biomass –project schedule is January 2012 – December 2013.
Efficient production and use of energy are key factors for sustainable economy and welfare. Local energy sources offer a diverse range of possibilities for the energy production and the areas will have better possibilities to be self-sufficient on energy.


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ZeniMax Files Injunction To Stop Oculus From Selling VR Headsets (gamespot.com) 77
ZeniMax, the parent company of Fallout and Skyrim developer Bethesda, has filed for an injunction against virtual-reality company Oculus over the recent stolen technology case. The company had accused Oculus of stealing VR-related code, and was subsequently awarded $500 million by a Dallas court earlier this month. ZeniMax has now filed additional papers against Oculus, requesting that Oculus' products using the stolen code be removed from sale. GameSpot reports: Specifically, ZeniMax is seeking to block sales of its mobile and PC developer kits, as well as technology allowing the integration of Oculus Rift with development engines Unreal and Unity, reports Law360. If the injunction isn't granted, ZeniMax wants a share of "revenues derived from products incorporating its intellectual properties," suggesting a 20 percent cut for at least 10 years. ZeniMax argues the previous settlement of $500 million is "insufficient incentive for [Oculus] to cease infringing." Oculus, meanwhile, says that "ZeniMax's motion does not change the fact that the [original] verdict was legally flawed and factually unwarranted. We look forward to filing our own motion to set aside the jury's verdict and, if necessary, filing an appeal that will allow us to put this litigation behind us," the virtual reality company stated.


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Top Definition
The action made popular in Japan, in which one person holds a hidden camera and another, usually masked, coordinate to; 1) pull an unsuspecting woman's shirt down exposing their breast or; 2) lift an unsuspecting woman's skirt and pulls down their underwear in a public place with the sole purpose to film and embarrass the women.
A man runs up to a woman in the street and rips down her shirt while his friend films the Japanese Sharking.
by Draven99984 July 18, 2008
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- 02-03-2014, 02:55 AM Thread Author #1
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Which phone is best for me?
I am looking into getting a new phone. I recently got my first smartphone, a Nokia Lumia 520, and I hate it. After doing some research, I think I want an android. But which one? Ideally, it would have a slider keyboard and both facing cameras. I've learned it should be at least running android 4.0 to run the latest apps. Is Kit Kat really worth it? It would need to be as cheap as possible too. Definitely not over $200. I am with AT&T's prepaid plan. My Nokia has a micro SIM, so if I could just take the SIM out of it and put it into my new phone that'd be good. I looked into the Droid 4, but it is only for Verizon. Is it possible to unlock it so I can use it on AT&T?
Thanks so much for your help!
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Re: Which phone is best for me?
I don't think there are many phones out there with a physical keyboard left. That right there is a dying breed.
As for apps, you care correct, many will now require to run on 4.0+. This will be relatively easy to find, as most phones now are coming out with some version of Jelly Bean (4.1, 4.2, or 4.3)
I am not sure if the Droid can run on AT&T. However, here is a device that I would suggest. The Moto G. It goes for less than $200 and can run on your network.
That is the 8GB version. The 16 is out of stock. With the 8GB, you should have 4 GB left for you to use (4 needs to be taken up by the OS).
There is a 16 available, but it is out of stock at the moment. And it will make the price go up. Possibly breaking your $200 budget.
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WS Atkins admitted yesterday it is cutting staff numbers in the UK after reductions in government spending affected its highways business. The engineer, which works on major construction projects including the London 2012 Olympics site, also said that trading conditions had been difficult in Ireland and Portugal but that good opportunities existed in Scandinavia for its European business.
In an update covering the period since October, Atkins said that transport infrastructure work in Hong Kong and improving business in mainland China have pushed Asia Pacific trading ahead of expectations. Trading in North America and its energy business was steady and is improving in the Middle East, it added.
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Jim Carrey Clarifies Tiger Tweets: I Do Not Condone Infidelity
ET has the latest...
After taking to his Twitter regarding Tiger Woods' affairs, saying, "No wife is blind enough to miss that much infidelity," Jim Carrey is now clarifying his remarks.
"I want 2 make it CLEAR that I do not condone infidelity at all, but 2 some degree the responsibility 4 it is shared by both people," the actor says in his latest tweet.
On Friday, the star tweeted: "No wife is blind enough to miss that much infidelity. Elin had [to be] a willing participant on the ride [for] whatever reason."
Related stories: Tiger Woods Tees Off at MastersHTV: 'Real Housewife' Weighs in on Tiger Woods and Sandra Bullock


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Safe Online Payments
Long journey by bus from mumbai to kozhikode is made effortless by redBus, one of the eminent bus ticketing platform fulfilling most of the travel requirements. Numerous operators run their buses in this route creating ample amount of convenience for many people in India. Secured online ticket reservation process and easy booking techniques help every traveler to undergo smooth booking.
If you are also looking for return checkout, please ensure to checkKozhikode to Mumbai Bus Tickets.
You can now book route tickets from mumbai to kozhikode at redBus.in. Apply time to time redBus offers on online bus ticket booking. Make some money and memories too!
I booked from Mumbai to Kozhikode in kallada travels. But it was worst service I got connection bus Mumbai to Bangalore national travels. Then Bangalore to Kozhikode kallada travels itself. But I paid 4000 rupees. But for national travels its 1800 andfrom Bangalore to Kozhikode 1000 rs. All together only 3800 rs. But I paid 4000 rs where is my 1200 rs?
Sixth among the world's top ten commercial centers, Mumbai generates 5% of India's GDP. Apart from having leading financial Institutions and many business conglomerates it also is the headquarter of Indian Entertainment Industry, with bollywood stars residing there which is also an attraction for tourists. Business opportunities, as well as the potential for a higher standard of living, attract migrants and it's rightly called "The City of Dreams". Though the best time to visit is between November and February, tourists visit all the year round .Mumbai's culture offers a cosmopolitan lifestyle with a variety of food, entertainment and the city rarely sleeps at night.


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Carhartt Women's Modern-Fit Jean-Straight Leg (Style #WB018)
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Raft from just £38pp
with March MayhemFind out more
You'll find gallons of fun at Lee Valley White Water Centre, from white water rafting on the Olympic course to family paddling on our lake. Choose from a range of activities or sit back and watch the action at The Terrace Bar & Café; you're guaranteed an unforgettable day out at this London 2012 venue.
Tick us off your bucket list with Fast & Furious Fridays
Celebrate with friends before the wedding, white water style!
We're not just wild about white water… The Terrace Bar & Café is the perfect chill out spot and you can watch the action without dipping your toe in the water! Plus our range of events and activities throughout the year will keep the whole family entertained.
If that's not enough to keep you and the little ones busy, there's acres of fun to explore just a hop, skip and jump away in the beautiful River Lee Country Park!
Support Dementia UK with a cuppa and some cake for just £4!
Enjoy a treat with the number one lady in your life.


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i was upon the donor list
© Copyright 2017 moonphish. All rights reserved.
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So the true cry leads to the boffo cry. Classic moonphish - my man!
All regards, Wilbur
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The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia
- Adetola B Adesida1,
- Lisa M Grady1,
- Wasim S Khan1 and
- Timothy E Hardingham1Email author
DOI: 10.1186/ar1929
© Adesida et al.; licensee BioMed Central Ltd. 2006
Received: 3 January 2006
Accepted: 21 February 2006
Published: 17 March 2006
Human meniscus cells have a predominantly fibrogenic pattern of gene expression, but like chondrocytes they proliferate in monolayer culture and lose the expression of type II collagen. We have investigated the potential of human meniscus cells, which were expanded with or without fibroblast growth factor 2 (FGF2), to produce matrix in three-dimensional cell aggregate cultures with a chondrogenic medium at low (5%) and normal (20%) oxygen tension. The presence of FGF2 during the expansion of meniscus cells enhanced the re-expression of type II collagen 200-fold in subsequent three-dimensional cell aggregate cultures. This was increased further (400-fold) by culture in 5% oxygen. Cell aggregates of FGF2-expanded meniscus cells accumulated more proteoglycan (total glycosaminoglycan) over 14 days and deposited a collagen II-rich matrix. The gene expression of matrix-associated proteoglycans (biglycan and fibromodulin) was also increased by FGF2 and hypoxia. Meniscus cells after expansion in monolayer can therefore respond to chondrogenic signals, and this is enhanced by FGF2 during expansion and low oxygen tension during aggregate cultures.
The meniscus is a fibrocartilaginous tissue found within the knee joint; it is responsible for shock absorption, load distribution, joint stability and protection of the articular cartilage [1–3]. Unfortunately, the reparative ability of the meniscus is limited, and injuries to the tissue are often treated by partial or total menisectomy, which is known to be associated with detrimental changes in joint function and high incidence of early osteoarthritis [4, 5]. Cell-based tissue engineering strategies have been proposed for the generation of meniscus substitute to aid repair of the tissue [6–10]. Like most musculoskeletal tissues, the biomechanical and functional properties of the meniscus depend on the composition and organization of its extracellular matrix (ECM) [1, 11]. The cells that produce and maintain this ECM have been called fibrochondrocytes [12]. Although the predominant morphology of these cells resembles that of the chondrocytes of articular cartilage [1], they produce predominantly type I collagen with smaller amounts of types II, III, V and VI collagens, and small amounts of aggrecan [13]. Isolated primary human meniscus cells show some characteristics similar to those of chondrocytes because during expansion in monolayer culture there is a sharp decrease in the expression of collagen type II and a change to predominantly fibroblast-like morphology [7]. This decline in type II collagen expression is reminiscent of the loss of differentiated phenotype of articular chondrocytes, and the use of these cells for tissue regeneration of meniscus might lead to the production of ECM of inferior biomechanical properties. Several investigators have reported strategies to restore the matrix-forming phenotype of articular chondrocytes. These include culturing chondrocytes at high cell densities to prevent cell flattening [14], in alginate gels [15] to retain the round chondrocytic morphology, in liquid suspension or in the presence of actin-disrupting agents, in the presence of fibroblast growth factor 2 (FGF2) [16], retroviral transduction with Sry-related high-mobility group (HMG) box-9 (SOX9) [17], in three-dimensional (3D) cell aggregate cultures with chondrogenic stimuli [18] and low oxygen tension (mild hypoxia) [19–21].
In the present study we have investigated the presence of chondrogenic growth factors and hypoxia with human meniscus cells expanded in monolayer culture to determine their chondrogenic potential. The effect of FGF2 on chondrogenic potential of meniscus cells was particularly of interest, because it had been shown to stimulate the growth [22] of meniscus cells in vitro and also to maintain the ability of monolayer expanded chondrocytes to redifferentiate [16, 23].
Materials and methods
Cell isolation and expansion
With informed consent, full-thickness meniscus was harvested aseptically from the tibial plateau of patients (aged 48 to 69 years) undergoing total knee replacements. Meniscus cells were released by incubation for 16 hours at 37°C in type II collagenase (0.2% w/v) in a standard medium, DMEM supplemented with 10% FCS, 100 units/ml penicillin and 100 units/ml streptomycin (all from Cambrex, Wokingham, UK), with l-glutamine (2 mM). Isolated cells were plated at 104 cells/cm2 and cultured in standard medium with or without FGF2 (5 ng/ml) (human recombinant; R&D systems, Abingdon, UK (added after overnight cell adherence) at 37°C and 20% O2. After about 2 weeks, when cells were subconfluent, first-passage (P1) cells were detached with trypsin-EDTA (Invitrogen, Paisley, Renfrewshire, UK) and split at a 1:2 ratio; culture was continued to produce second-passage (P2) cells, which were used for experiments. Doubling times of P1 and P2 human meniscus cells were obtained by plating P1 and P2 meniscus cells at 5 × 105 cells in 75 cm2 tissue culture flasks in the presence and absence of FGF2 (5 ng/ml). Meniscus cell number was evaluated at regular timed intervals in quadruplicate by cell counting after treatment with trypsin. The doubling time of a cell population during the exponential growth phase was calculated as the slope of T against ln N/N0), where N0 and N are the cell number at the beginning and end of the exponential growth time (T), respectively [16].
Three-dimensional cell aggregate cultures of meniscus cells were formed by the centrifugation of 5 × 105 cells in 15 ml conical culture tubes (Corning, Loughborough, UK) at 1,200 r.p.m. for 5 minutes. The cell aggregates were cultured in 0.5 ml of DMEM supplemented with chondrogenic factors, namely ITS+1, 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid 10 nM dexamethasone, 10 ng/ml transforming growth factor β3, 25 μg/ml ascorbate 2-phosphate (all from Sigma, Poole, UK) with 10% FCS for 14 days with 5% CO2 under normal oxygen (20% O2) or low oxygen tension (5% O2) at 37°C. At the end of the culture period, the wet weights of cell aggregates were recorded. Control monolayer cultures of meniscus cells with or without FGF2 expansion (R&D systems) were set up in six-well plates at a 105 cells per well in standard medium. Monolayer controls were similarly cultured for 14 days under normoxic and hypoxic culture conditions, with standard medium change every 2 days.
Gene expression analysis
Total RNA was prepared from whole tissue, monolayer cells and cell aggregate cultures with the use of Tri-Reagent (Sigma). Total RNA from tissue was isolated after homogenization with a Braun Mikrodismembranator. Cell aggregate cultures were ground up in the Tri-Reagent with Molecular Grinding Resin (Geno Technology Inc., St Louis, MO, USA). To minimize any changes in gene expression, cultures caps were closed before removal from the low-oxygen incubator, and cell aggregates and monolayers were immediately (less than 1 minute) transferred into Tri-reagent. cDNA was synthesised from 10 to 100 ng of total RNA with the use of global amplification methodology [24]. Globally amplified cDNAs were diluted 1:1000 and 1 μl aliquots of the diluted cDNA were amplified by polymerase chain reaction in a 25 μl reaction volume in an MJ Research Opticon 2 real-time thermocycler with a SYBR Green Core Kit (Eurogentec, Seraing, Belgium), with gene-specific primers designed by using ABI Primer Express software. Relative expression levels were normalised with β-actin and calculated with the use of the 2-ΔCtmethod [25]. All primers were from Invitrogen. All primer sequences were designed on the basis of human sequences as follows: aggrecan, 5'-AGGGCGAGTGGAATGATGTT-3' (forward) and 5'-GGTGGCTGTGCCCTTTTTAC (reverse); β-actin, 5'-AAGCCACCCCACTTCTCTCTAA-3' (forward) and 5'-AATGCTATCACCTCCCCTGTGT-3' (reverse); biglycan, 5'-TTGCCCCCAAACCTGTACTG-3' (forward) and 5'-AAAACCGGTGTCTGGGACTCT-3' (reverse); COL1A2, (collagen) 5'-TTGCCCAAAGTTGTCCTCTTCT-3' (forward) and 5'-AGCTTCTGTGGAACCATGGAA-3' (reverse); COL2A1, 5'-CTGCAAAATAAAATCTCGGTGTTCT-3' (forward) and 5'-GGGCATTTGACTCACACCAGT-3' (reverse); COL3A1, 5'-GGCATGCCACAGGGATTCT-3' (forward) and 5'-GCAGCCCCATAATTTGGTTTT-3' (reverse); decorin, 5'-CAAGCTTAATTGTTAATATTCCCTAAACAC-3' (forward) and 5'-ATTTTATGAAGGGAGAAGACATTGGTTTGTTGACA-3' (reverse); fibromodulin, 5'-TGAAGCACCTTCCCTGAGAAG-3' (forward) and 5'-GGTTTGGCTTTTGTGGATTCC-3' (reverse); Sry-related HMG box-5 (long form), L-SOX5 5'-GAATGTGATGGGACTGCTTATGTAGA-3' (forward) and 5'-GCATTTATTTGTACAGGCCCTACAA-3' (reverse); Sry-related HMG box-6 (SOX6), 5'-CACCAGATATCGACAGAGTGGTCTT-3' (forward) and 5'-CAGGGTTAAAGGCAAAGGGATAA-3' (reverse); SOX9, 5'-CTTTGGTTTGTGTTCGTGTTTTG-3' (forward) and 5'-AGAGAAAGAAAAAGGGAAAGGTAAGTTT-3' (reverse).
Biochemical analysis of cell aggregate cultures
After culture, cell aggregates were digested overnight in 20 μl of 10 units/ml papain (Sigma), 0.1 M sodium acetate, 2.4 mM EDTA, 5 mM l-cysteine, pH 5.8, at 60°C. The DNA content of the papain digest was determined by measuring Hoechst 33258 dye (Sigma) binding with a Hoeffer Dyna Quant 200 fluorometer. Glycosaminoglycans were assayed in the papain digest by using 1,9-dimethylmethylene blue (Aldrich, Poole, UK) [26, 27] with shark chondroitin sulphate (Sigma) as standard.
Histology and immunohistochemistry
Cell aggregates were fixed in 4% formaldehyde and embedded in paraffin wax; 5 μm sections were cut and stained with 0.1% safranin-O. For immunohistochemical analysis, sections were digested with chondroitinase ABC and then incubated with antibodies against collagen I (sc-8786) or collagen II (sc-7764) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Immunolocalised antigens were visualised with a biotin-conjugated donkey anti-goat secondary antibody (Santa Cruz Biotechnology) and a streptavidin-horseradish peroxidase or anti-rabbit horseradish peroxidase (Sigma) labelling kit with 3,3'-diaminobenzidine (Dako, Ely, UK). Images were captured with an Axioplan 2 (Carl Zeiss Ltd, Welwyn Garden City, UK) microscope and an AxioCam HRc camera (Carl Zeiss), with AxioVision 4.3 software (Carl Zeiss).
Statistical analysis
Experiments were repeated in triplicate with cells from the same donor. Gene expression data are presented as the mean ± SD (standard deviation) for the replicates. Statistical significance differences between the gene expression values of normoxia and hypoxia cell aggregate cultures were determined with Student's unpaired t test.
Cell population doubling during monolayer expansion of meniscus cells
Effect of FGF2 expansion on chondrogenic response of human meniscus cells
The combination of FGF2 expansion and hypoxia therefore increased the capacity of meniscus cells to re-express the matrix-forming phenotype of meniscus cells. Comparison of these expression levels with cells in tissue showed that COL1A2 expression in FGF2-derived cell aggregates under low oxygen was 5-fold less than its expression in tissue, while in non-FGF2-derived cell aggregates under low oxygen COL1A2 expression was decreased 10-fold relative to its expression in tissue. In addition, FGF2 expansion increased the expression of COL2A1 in cell aggregates under low oxygen 5-fold relative to COL2A1 expression in tissue (Figure 3). However, in view of these changes in COL2A1 expression, it was surprising that the expression of SOX9 in tissue was 7-fold that in cell aggregates formed from FGF2-expanded cells (Figures 2 and 3).
COL3A1, which is not expressed in normal cartilage but is expressed in meniscus, was expressed similarly in cell aggregates derived from FGF2-expanded and non-FGF2-expanded cells regardless of oxygen tension, and the expression of COL3A1 in tissue and in the cell aggregates were at similar levels (Figure 3).
Effect of FGF2 expansion on matrix formation and proteoglycan matrix deposition
Effect of FGF2 expansion on collagen deposition
Cell-based strategies to engineer a meniscus substitute has been suggested as an approach to the treatment of meniscal defects. However, attempts to expand human meniscus cells in monolayer culture have resulted in decreased gene expression of ECM components of importance in meniscus function, such as type II collagen [7], which is located mostly in the inner region of the tissue and is thought to endow properties suitable for compressive load distribution [13]. In this study we have investigated the combination of culture under conditions of low oxygen tension and FGF2-stimulated cell expansion as a strategy to augment the re-expression of type II collagen and a matrix-forming phenotype in human meniscus cells. Human meniscus cells showed a chondrogenic response (increased collagen II gene and protein expression) when cultured in cell aggregates regardless of FGF2 presence or absence during monolayer expansion (Figure 3). However, the response was much greater in cell aggregate cultures derived from FGF2-expanded cells (Figure 3). The type II collagen protein was notably more localized in the matrix at the periphery of the cell aggregates and more pericellularly at the central region of the cell aggregates (Figure 8). The chondrogenic response was further enhanced by low oxygen tension, which caused increased gene expression of SOX9. However, the expression of L-SOX5 and SOX6 remained unchanged and low. This was surprising because L-SOX5 and SOX6 interact cooperatively with SOX9 to promote the expression of cartilage-specific genes (such as those encoding COL2A1 and aggrecan) [28]. The enhanced chondrogenic response at low oxygen tension may involve the transcriptional activity of HIF-1α, (hypoxia inducible factor) which modulates the expression of a variety of hypoxia-inducible genes under low oxygen tension [32]. It has been reported that hypoxia promotes the differentiation of murine mesenchymal stroma cells along a chondrocyte pathway in part by activating SOX9 via a HIF-1α-dependent mechanism [33]. Furthermore, HIF-1α has been shown to bind to cAMP-response element-binding protein (CREB)-binding protein (CBP)/p300 [34], which SOX9 uses to exert its cartilage-specific type II collagen gene promoter activity [35]. It was noticeable that in monolayer there was no significant chondrogenic response in changing from normal oxygen tension (20%) to low oxygen tension (5%) compared with changing from monolayer to aggregate (Figure 3). In the comparison between the expression of cells in aggregates and in monolayer, the 3D structure of a cell aggregate, together with oxygen consumption by the cells, would result in a lower oxygen tension within the aggregate than in cells in a monolayer. However, because cell aggregates showed a strong chondrogenic response at 5% and 20% oxygen, any small difference in oxygen tension was clearly not a major factor driving the chondrogenic response.
It was notable that the high gene expression of COL1A2 in cell aggregates formed from FGF2-expanded cells was not correlated with the matrix immunostaining, which was weak with anti-type I collagen. This suggested that there is a more complex control of type I collagen translation, fibrillogenesis and matrix deposition.
Further characterization of the chondrogenic response by human meniscus cells was by gene expression analysis of proteoglycan common to cartilage and meniscus. Aggrecan gene expression was low in meniscal cells and was not influenced by FGF2-mediated cell expansion, but its expression increased in cell aggregate cultures. FGF2-expanded cells expressed higher levels of biglycan and fibromodulin in cell aggregates, and this was unaffected by low oxygen. In non-FGF2-expanded cells, biglycan and fibromodulin expression was similar in monolayer and cell aggregates, but biglycan was increased by low oxygen tension in cell aggregates formed from FGF2-expanded cells. Histology showed an increase in safranin-O staining in cell aggregates formed from FGF2-expanded cells at low oxygen tension. Although this did not reflect a significant statistical increase in GAG/DNA ratio under low-oxygen conditions, the cell aggregates formed were of higher wet weight and this might correspond to a greater increase in cell number.
This study showed that P2 meniscus cells after growth stimulation with FGF2 were able to re-express type II collagen and proteoglycans at both the gene and protein levels. Furthermore, this ability was enhanced by 5% oxygen culture conditions and was higher than with meniscus cells expanded in the absence of FGF2. The cells used in this study were from all regions of the meniscus and thus include cells from the inner avascular region, which contains more collagen type II than the outer vascular region. FGF2 may favour the selective proliferation of the cells from this region and thus sustain the population of meniscus cells with chondrogenic potential. Expansion with FGF2 has been reported to increase the chondrogenic potential of human bone marrow stromal cells [36]. Previous studies by Nakata and colleagues [7] have reported three distinguishable cell types within the human meniscus tissue: small round chondrocyte-like cells, elongated fibroblast-like cells and polygonal cells; they related the loss of collagen II expression in meniscus cells during monolayer expansion with the gradual loss of both the chondrocyte-like and polygonal cell populations to leave predominantly fibroblast-like cells. The mechanism by which FGF2 conferred this ability to re-express type II collagen and proteoglycan in meniscus cells is therefore either by the selective proliferation of chondrogenic cells within the culture or by maintaining the cells in a more plastic and responsive state to chondrogenic stimuli [16].
We have shown that the loss of collagen II expression after monolayer expansion of human meniscus cells can be circumvented by adding FGF2 during the monolayer expansion phase. Furthermore, the ability of FGF2-expanded meniscus cells to re-express a matrix rich in collagen I and II is enhanced by hypoxia. This combination strategy may improve cell-based approaches to generate the biomechanical properties of meniscus substitutes.
= three-dimensional
= collagen
= Dulbecco's modified Eagle's medium
= extracellular matrix
= fetal calf serum
= fibroblast growth factor 2
= glycosaminoglycan
= hypoxia inducible factor
= high-mobility group
= Sry-related HMG box-5 (long form)
= Sry-related HMG box-6
= Sry-related HMG box-9.
We wish to thank Dr Simon Tew and Dr SJ Millward-Sadler (University of Manchester) for helpful scientific and technical discussions, and Dr Ann Canfield (University of Manchester) for access to the hypoxia incubator. This work was supported by grants from the European Framework V Program (Meniscus Regeneration Project Contract GRD-CT-2002-00703).
Authors' Affiliations
- Fithian DC, Kelly MA, Mow VC: Material properties and structure-function relationships in the menisci. Clin Orthop. 1990, 252: 19-31.PubMedGoogle Scholar
- Ahmed AM: The load-bearing role of the knee meniscus. Knee Meniscus: Basic and Clinical Foundations. Edited by: Mow VC, Jackson DW. 1992, New York: Raven Press, 59-73.Google Scholar
- Levy IM, Torzilli PA, Fisch ID: The contribution of the menisci to the stability of the knee. Knee Meniscus: Basic and Clinical Foundations. Edited by: Mow VC, Jackson DW. 1992, New York: Raven Press, 107-115.Google Scholar
- Fairbank T: Knee joint changes after menisectomy. J Bone Joint Surg. 1948, 30B: 664-670.Google Scholar
- Cox JS, Nye CE, Schaefer WW, Woodstein IJ: The degenerative effects of partial and total resection of the medial meniscus in dogs' knees. Clin Orthop Relat Res. 1975, 109: 178-183.View ArticlePubMedGoogle Scholar
- Ibarra C, Koski JA, Warren RF: Tissue engineering meniscus: cells and matrix. Orthop Clin North Am. 2000, 31: 411-418. 10.1016/S0030-5898(05)70160-7.View ArticlePubMedGoogle Scholar
- Nakata K, Shino K, Hamada M, Mae T, Miyama T, Shinjo H, Horibe S, Tada K, Ochi T, Yoshikawa H: Human meniscus cell: characterization of the primary culture and use for tissue engineering. Clin Orthop. 2001, 391 (Suppl): S208-S218.View ArticlePubMedGoogle Scholar
- Buma P, Ramrattan NN, van Tienen TG, Veth RPH: Tissue engineering of the meniscus. Biomaterials. 2004, 25: 1523-1532. 10.1016/S0142-9612(03)00499-X.View ArticlePubMedGoogle Scholar
- Adams SB, Randolph MA, Gill TJ: Tissue engineering for meniscus repair. J Knee Surg. 2005, 18: 25-30.PubMedGoogle Scholar
- Sweigart MA, Athanasiou KA: Toward tissue engineering of the knee meniscus. Tissue Eng. 2001, 7: 111-129. 10.1089/107632701300062697.View ArticlePubMedGoogle Scholar
- Adams ME, Hukins DWL: The extracellular matrix of the meniscus. Knee Meniscus: Basic and Clinical Foundations. Edited by: Mow VC, Jackson DW. 1992, New York: Raven Press, 15-28.Google Scholar
- McDevitt CA, Miller RR, Spindler KP: The cells and cell matrix interactions of the meniscus. Knee Meniscus: Basic and Clinical Foundations. Edited by: Mow VC, Jackson DW. 1992, New York: Raven Press, 29-36.Google Scholar
- Tanaka T, Fujii K, Kumagae Y: Comparison of biochemical characteristics of cultured fibrochondrocytes isolated from the inner and outer regions of human meniscus. Knee Surg Sports Traumatol Arthrosc. 1999, 7: 75-80. 10.1007/s001670050125.View ArticlePubMedGoogle Scholar
- Watt FM: Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture. J Cell Sci. 1988, 89: 373-378.PubMedGoogle Scholar
- Benya PD, Shaffer JD: Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell. 1982, 30: 215-224. 10.1016/0092-8674(82)90027-7.View ArticlePubMedGoogle Scholar
- Martin I, Vunjak-Novakovic G, Yang J, Langer R, Freed LE: Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Exp Cell Res. 1999, 253: 681-688. 10.1006/excr.1999.4708.View ArticlePubMedGoogle Scholar
- Tew SR, Li Y, Pothacharoen P, Tweats LM, Hawkins RE, Hardingham TE: Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthritis Cartilage. 2005, 13: 80-89. 10.1016/j.joca.2004.10.011.View ArticlePubMedGoogle Scholar
- Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res. 1998, 238: 265-272. 10.1006/excr.1997.3858.View ArticlePubMedGoogle Scholar
- Murphy CL, Polak JM: Control of human articular chondrocyte differentiation by reduced oxygen tension. J Cell Physiol. 2004, 199: 451-459. 10.1002/jcp.10481.View ArticlePubMedGoogle Scholar
- Murphy CL, Sambanis A: Effect of oxygen tension and alginate encapsulation on restoration of the differentiated phenotype of passaged chondrocytes. Tissue Eng. 2001, 7: 791-803. 10.1089/107632701753337735.View ArticlePubMedGoogle Scholar
- Grimshaw MJ, Mason RM: Modulation of bovine articular chondrocyte gene expression in vitro by oxygen tension. Osteoarthritis Cartilage. 2001, 9: 357-364. 10.1053/joca.2000.0396.View ArticlePubMedGoogle Scholar
- Webber RJ, Harris MG, Hough AJ: Cell culture of rabbit meniscal fibrochondrocytes: proliferative and synthetic response to growth factors and ascorbate. J Orthop Res. 1985, 3: 36-42. 10.1002/jor.1100030104.View ArticlePubMedGoogle Scholar
- Martin I, Suetterlin R, Baschong W, Heberer M, Vunjak-Novakovic G, Freed LE, Jakob M, Demarteau O, Schafer D, Hintermann B, et al: Enhanced cartilage tissue engineering by sequential exposure of chondrocytes to FGF-2 during 2D expansion and BMP-2 during 3D cultivation. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro. J Cell Biochem. 2001, 83: 121-128. 10.1002/jcb.1203.View ArticlePubMedGoogle Scholar
- Al-Taher A, Bashein A, Nolan T, Hollingsworth M, Brady G: Global cDNA amplification combined with real-time RT-PCR: accurate quantification of multiple human potassium channel genes at the single cell level. Yeast. 2000, 17: 201-210. 10.1002/1097-0061(20000930)17:3<201::AID-YEA30>3.0.CO;2-R.PubMed CentralView ArticlePubMedGoogle Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.View ArticlePubMedGoogle Scholar
- Ratcliffe A, Doherty M, Maini RN, Hardingham TE: Increased concentrations of proteoglycan components in the synovial fluids of patients with acute but not chronic joint disease. Ann Rheum Dis. 1988, 47: 826-832.PubMed CentralView ArticlePubMedGoogle Scholar
- Farndale RW, Buttle DJ, Barrett AJ: Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta. 1986, 883: 173-177.View ArticlePubMedGoogle Scholar
- Lefebvre V, Li P, de Crombrugghe B: A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene. EMBO J. 1998, 17: 5718-5733. 10.1093/emboj/17.19.5718.PubMed CentralView ArticlePubMedGoogle Scholar
- Djurasovic M, Aldridge JW, Grumbles R, Rosenwasser MP, Howell D, Ratcliffe A: Knee joint immobilization decreases aggrecan gene expression in the meniscus. Am J Sports Med. 1998, 26: 460-466.PubMedGoogle Scholar
- Messner K, Gao J: The menisci of the knee joint. Anatomical and functional characteristics, and a rationale for clinical treatment. J Anat. 1998, 193: 161-178. 10.1046/j.1469-7580.1998.19320161.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Scott PG, Nakano T, Dodd CM: Isolation and characterization of small proteoglycans from different zones of the porcine knee meniscus. Biochim Biophys Acta. 1997, 1336: 254-262.View ArticlePubMedGoogle Scholar
- Semenza GL: HIF-1 and mechanisms of hypoxia sensing. Curr Opin Cell Biol. 2001, 13: 167-171. 10.1016/S0955-0674(00)00194-0.View ArticlePubMedGoogle Scholar
- Robins JC, Akeno N, Mukherjee A, Dalal RR, Aronow BJ, Koopman P, Clemens TL: Hypoxia induces chondrocyte-specific gene expression in mesenchymal cells in association with transcriptional activation of Sox9. Bone. 2005, 37: 313-322. 10.1016/j.bone.2005.04.040.View ArticlePubMedGoogle Scholar
- Ebert BL, Bunn HF: Regulation of transcription by hypoxia requires a multiprotein complex that includes hypoxia-inducible factor 1, an adjacent transcription factor, and p300/CREB binding protein. Mol Cell Biol. 1998, 18: 4089-4096.PubMed CentralView ArticlePubMedGoogle Scholar
- Tsuda M, Takahashi S, Takahashi Y, Asahara H: Transcriptional co-activators CREB-binding protein and p300 regulate chondrocyte-specific gene expression via association with Sox9. J Biol Chem. 2003, 278: 27224-27229. 10.1074/jbc.M303471200.View ArticlePubMedGoogle Scholar
- Mastrogiacomo M, Cancedda R, Quarto R: Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells. Osteoarthritis Cartilage. 2001, 9 (Suppl A): S36-S40. 10.1053/joca.2001.0442.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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By Kelli Acciardo | 01/22/2014 - 09:35
Zoe Saldana has been looking amazing all weekend in Park City, Utah.
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By Cristina Arreola | 01/14/2014 - 09:50
Sofia Vergara may have not have walked away with a Golden Globe on Sunday night, but the Colombiana certainly made the most of the awards show.
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By Samantha Leal | 01/12/2014 - 12:00
Sofia Vergara knows a thing or two about dressing your body. With her Kmart line, she makes her fun (and sexy) style affordable and chic.
Read more
By Ana Luisa Suarez | 01/03/2014 - 11:01
Happy New Year! 2013 is behind us and we're ready for everything 2014 has to offer. We're hoping that includes some awesome new fashion trends and beauty looks.
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By Latina Staff | 12/30/2013 - 16:05
We can't deny it: Naya Rivera has some killer style! The bride-to-be was recently spotted out wedding dress shopping wearing a very chic, capped sleeve dress in a sweet gray rose design.
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By Cristina Arreola | 12/27/2013 - 10:23
Christmas is over...but we're still feeling the holiday spirit for this week's Latina Style Wars!
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By Ana Luisa Suarez | 11/27/2013 - 11:46
Thanksgiving is finally here! It's a time for great good, family, and being thankful for all that you have. It's also the perfect opportunity to dress up and look your best! For this week's edition of Latina Style Wars, we asked you to send in your favorite outfit from a previous Thanksgiving.
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By Cristina Arreola | 11/08/2013 - 09:45
Selena Gomez is all grown up!
In the interior spread of Flaunt Magazine, the "Slow Down" singer goes high-fashion in a trendy bobbed wig with bangs.
The mature look is an upgrade from her Disney days, and the interview with the star further proves that Gomez is no longer a teen pop star.
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By Ana Luisa Suarez | 11/01/2013 - 15:41
Fall is in full swing now that Halloween has officially wrapped up and winter weather will be here soon enough. So for this week's edition of Latina Style Wars, we wanted to see how you're keeping warm! We asked for pictures of our favorite fall accessories: scarves! And the submissions we got made us want to run out and buy more.
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By Samantha Leal | 11/01/2013 - 10:47
From charity events to award shows, these Latina celebrities rocked the red carpet and stage. But who looked the best? See the list of the most stylish ladies from this week:
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The Texas Workforce Commission provides employment, training and related services to veterans via a network of partnerships,
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Contact Information: 1700 No. Congress Avenue, Suite 800 Austin Texas, 78701-1495 (800) 252-8387
The City of Houston Office of Veterans Affairs
Services: Transition Assistance Program (TAP) Seminars with military units, local law enforcement
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Best Approximation of Love Story by a Boulder Author
God-Shaped Hole, Tiffanie Debartolo's tale of star-crossed love - born in the classified ads, played out beneath the artificial glow of Los Angeles life -- has all of the elements of pure romantic noir: The lead character, Trixie, has a love affair with the dreamy and intense Jacob, a writer for an alternative weekly newspaper, that is burning, tumultuous and, ultimately, tragic. (Let's just say someone drowns in the Pacific, leaving the other for the great wave in the sky.) But the debut novel from DeBartolo, who also penned the screenplay for the Jennifer Aniston/Ione Sky vehicle Dreams of an Insomniac, is funny, bold, quasi-philosophical and a hell of a lot smarter than your standard grocery-store paperback fare. Currently splitting time between Boulder and the Big Apple, DeBartolo is reportedly at work on her second book; we can only hope it's as divine as her first.
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Green Helmets: Eco-Intervention in the Twenty-First Century Unilateral and Multilateral Intervention
Linda A. Malone
Proceedings of the Annual Meeting (American Society of International Law)
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text: Sarah Palin Will Not Run For Re-Election, Resigning As Alaska's GovernorHortense Smith7/03/09 3:25pmFiled to: Breakingsarah palinSarah palin not running for reelectionPoliticsGettypicSarah palin resigningTop352EditPromoteShare to KinjaToggle Conversation toolsGo to permalink GOP sources are claiming that former Vice Presidential candidate Governor Sarah Palin will not be seeking a second term as Alaska's governor and will actually be resigning from her position in a matter of weeks. Advertisement "She thinks she has accomplished goals she has set forward," a source tells CNN, "She sees what a positive influence she has had on people's lives from traveling the country in the last year." Tina Fey, get your costume ready, because I think the "Also, You Betcha! Tour: 2009-2012" is about to begin.Palin Will Not Run For Re-Election [CNN] Palin Won't Run For Re-Election [WDSU] Report: Palin Resigning As Alaska Gov [MSNBC] Transcript Of Palin's Resignation [Alaska.Gov]Reply35 repliesLeave a reply


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Byron Bader
December 12 2013 01:29PM
- stick tap to @Notbaertschi for grabbing this beauty.
The Flames are two days removed from an emotional game against the Bruins on Tuesday, that saw the return of Jarome Iginla to the Saddledome for the first time since he was traded last Spring. After the emotions settled after a raucous ovation to start things off, the Bruins eventually got their game going in the 3rd period, beating the Flames 2-1.
Breaking: Flames Fire Feaster & Weisbrod
Ryan Pike
December 12 2013 10:18AM
The Flames official Twitter account has announced that Jay Feaster and John Weisbrod have been relieved of their duties.
A press conference with real, actual details is coming up at 11am.
Five things: A lovefest
Ryan Lambert
December 12 2013 08:24AM
1. The homecoming
Nice to see the big, long ceremony for Jarome Iginla, who deserved bigger and longer if anything. If that standing ovation before the game lasted all night and they didn't play the game, that might have been fitting. You really can't say enough good things.
The "Iginla's Return" FN Roundtable
Ryan Pike
December 11 2013 01:53PM
With Jarome Iginla's emotional return to Calgary last night, the FlamesNation crew decided to revisit the Big Trade with the Penguns. As such, here's a quick run around the table to see how Iginla's remembered by this bunch and how things look for the Flames in the aftermath of his trade last March.
Fans Never Really Got to Say Good-Bye
Steve Macfarlane
December 11 2013 08:23AM
Jarome Iginla's last game as a member of the Flames was technically sitting out as as a healthy scratch last season, on the night he was ultimately traded. Because there were deals in the works, Iginla was told to stay home. He didn't even get to come to the rink.
For 16 years, Iginla was the future, then face of the franchise here. And just like that, he was gone.


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|Gravity – the most basic of all forces - is still surrounded by a great deal of mystery. A recent workshop hosted at SFU aimed to discover new ways of testing theories and ideas.
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|The Honourable Mike Lake visits TRIUMF
|15 January 2015
|On January 13, 2015, TRIUMF played host to the Honourable Mike Lake, Parliamentary Secretary to the Minister of Industry, and Member of Parliament from Edmonton.
|Current Events
|January 9 ARIEL Snapshot
|09 January 2015
|Documentation for the e-linac SR2 review is being completed. 10 kW beam dump, water piping and instrumentation work is on-going in the e-hall.
|Current Events
|December 19 ARIEL Snapshot
|21 December 2014
|Good progress towards Status Review (SR-2) preparation - on track for mid-January completion. SR-2 report and records will be reviewed by CNSC prior to resuming e-linac commissioning.
|Awards & Honours
|Photos Contemplate the Humanity of Science
|19 December 2014
|Whether in the form of a researcher's handwritten scribbles or through a photographer's creativity, photographers brought a fresh look to research at work at TRIUMF 3rd Photowalk.
|Current Events
|First Experiments Performed With GRIFFIN
|18 December 2014
|A state-of-the-art instrument for detecting and measuring gamma radiation with high efficiency and precision, GRIFFIN, became operational this year and ran its first experiments.


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Linkwise Technology Pte Ltd
Control, Backup, Power Supply
A companion product for the EMS system, this intelligent back-up battery will trigger an SMS alert to user in any case of power failure; making sure that user get notified of the alarm and at the same time providing at least 2 hours of power supply to the EMS unit
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External antenna designed to boost up the GSM signal in an enclosed area
Expands the input/output points of Message Master 4000 allowing monitoring of multiple equipment's.
Message Master 4000 sends SMS alerts and e-mail notification to users instantaneously when an alarm states occurred.
Aplusweb was specially designed to interface with any equipment or device to provide basic wireless SMS alerting
AplusWEB was specially designed to interface with any equipment or device to provide basic wireless SMS alerting
TS70 is used to measure space temperature in general temperature measurement applications
Picobox THS-90R is used to measure ambient temperature & humidity levels.
The ideal early detection of water sources prevent damage and downtime from water leaks & spills.
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