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H2O is the first to bring the FLEX car wash concept to Highlands Ranch, Colorado.
A FLEX car wash has Full Service, Self Service includes FREE vacuums and Express detail services all in one location. This allows H2O customers more options for the exact type of wash they need, hence the word FLEX.
While giving your car the best clean and shine, H2O recycles throughout our facility to help preserve Colorful Colorado. We have invested heavily in our technology and processes to insure we are environmentally friendly.
H2O Car Wash provides a great SHINE through excellence and convenience in car cleaning care with a friendly home-town environment.
Locally owned and operated. All of our partners, vendors and contractors are local to the Highlands Ranch market.
We take pride in helping our community!


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Minted Peas
|2 Tablespoon
|Chopped fresh mint
|1 Tablespoon
|1 Teaspoon
|Frozen peas
|10 Ounce (1 Package)
|1⁄2 Teaspoon
Place butter in 1-cup measure.
MICROWAVE on HIGH until butter melts.
Stir in mint leaves and sugar.
Place frozen peas in 1 -quart casserole.
Sprinkle with salt.
Pour minted butter over peas.
MICROWAVE 3 to 5 MINUTES on HIGH, or until peas are tender.
Stir well to coat with sauce.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size
Calories 116 Calories from Fat 56
% Daily Value*
Total Fat 6 g9.8%
Saturated Fat 3.9 g19.5%
Trans Fat 0 g
Cholesterol 16.1 mg5.4%
Sodium 320.8 mg13.4%
Total Carbohydrates 12 g3.8%
Dietary Fiber 3.5 g14%
Sugars 4.8 g
Protein 4 g7.8%
Vitamin A 36.1% Vitamin C 23.3%
Calcium 2.7% Iron 7.1%
*Based on a 2000 Calorie diet

subset: commoncrawl


text:
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5401 W. 20Th Street
Greeley, Colorado 80634
United States
We offer a range of professional development and personal enrichment courses and certificates, organizational workforce solutions at our site, your site, or online. At our Event Center you'll receive support for event planning and full service space renta ... more
Giselle Garcia Brabo
CVC Brasil Operadora e Agencia de Viagens S.A.
Lisa Brabo
The Family Services Agency Inc
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Michael Brabo
Gigamon LLC
Emily Brabo
St. Mark's Episcopal School


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We will be going live with Economy International shipping rates on Tuesday, October 11, and we will begin shipping International Economy on Wednesday, October 12. On top of Edge membership programs being offered to 28 countries ($29.95 – 39.95), all other international orders will be discounted up to 70% off of current rates.
We will also be offering free Economy International shipping on the following order sizes, site wide:
- $115+ Free Shipping to Canada & Mexico
- $150+ Free shipping to 26 Countries (Argentina, Australia, Austria, Belgium, Brazil, Denmark, Finland, France, Germany, Greece, Hong Kong, Israel, Italy, Japan, Netherlands, New Zealand, Norway, Poland, Portugal, Republic of Ireland, Singapore, South Korea, Spain, Sweden, Switzerland, United Kingdom (UK)
- $200+ Free Shipping to any other country we ship to not mentioned above
We're very excited about being able to offer this to our customers across the world. Specifically, a tremendous selection of Artisan soaps will finally have a way to cost effectively get into the hands of a large portion of the world. We think it will not only be great for our business, but will increase the exposure and business of some of the amazing products being produced by our community.


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Free personalized radio that
plays the music you love
Loved Me Back To Life
I couldn't get out
Turn the lights down
The voices inside are so loud.
I couldn't get out
Turn the lights down
The voices inside are so loud.
Features of This Trackelectronica influences
r & b influences
a subtle use of vocal counterpoint
a subtle use of vocal harmony
acoustic rhythm piano
use of a string ensemble
a clear focus on recording studio production
thru composed melodic style
mixed minor & major key tonality
an emotional female lead vocal performance
romantic lyrics
emphasis on instrumental arranging
and many other similarities identified in the Music Genome Project
These are just a few of the hundreds of attributes cataloged for this track by the Music Genome Project.show more
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I got a D in my JC english exan which I was dissapointed in a way and not. I basically waffled my way through it and i am beginning to find higher level Lc english difficult. Any thought's on what to do , to drop to ordinary or stay at higher??
ask your teacher for guidance , dont put extra stress on yourself x


text:
Is R K Aluminium incorrect or missing key business information? Update your Hotfrog listing today.
Contact details
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Hotfrog INDIA provides information regarding R K Aluminium in New Delhi Delhi. R K Aluminium is located at Main Delhi Road Opposite Dsids Office, New Delhi Delhi 110039 and provides Metals services. Contact them on 098119 74546.
Is R K Aluminium in Main Delhi Road Opposite Dsids Office, New Delhi Delhi 110039 your business? Claim your listing and attract more leads by adding more content, photos and other business details.
We have more Metals services in New Delhi Delhi available on Hotfrog INDIA. You can update your search for New Delhi Metals by location, keyword or service options.
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Craft Activities for Ages 3-5
Craft Activities for Ages 3-5
Use a flashlight to see shapes!
Dish out creativity with this rainy-day activity.
by Megan Pflug
Get in the swing of spring with this cheery craft.
Ready to craft your own custom kite? It's easy breezy!
Celebrate Mom with one or all of these handmade gifts.
Help your little one make stars that really sparkle!
Build a village of your own with our social studies activity.
Make a fun matching game with familiar faces.
A lesson in your own history is the secret treasure of this project.
by Risa Young
This treasure box is perfect for precious photos, special letters, found items, and more.
Bendable. Pokeable. Glueable. Cardboard is a versatile playtime possibility.
This easy Native American craft is a piece of history your child can use today.
These sewing cards are tailor-made for your toddler.
Make a traveling exhibition of your most prized works.


text:
Best Fishing Times: 5:54AM to 7:54AM, and 10:54PM to 12:54AM
Alternate Fishing Times: 10:24AM to 12:24PM
Fishing Little Flint Creek Dam, AR on 2/26/2017 will be best from 5:54AM through 7:54AM, and from 10:54PM to 12:54AM. If those times won't work for you, 10:24AM to 12:24PM looks promising. Remember to adjust these times based on barometric pressure, and weather changes.


text:
Costume Information
|Cost :
|Time Taken :
|2 hours
|Again to accompany my sister I decided to be ciel.
I think the wig makes my face look a bit funny! but oh well :)
So this was actually a pretty easy costume to do! I only had to make the eyepatch and the bloomers as I already had the hat, waistcoat, shirt and shoes that fitted ciel's victorian look anyway.
I was a bit of an oddly tall ciel however with the shoes I was wearing so I'm not too sure if i'll ever truely revive the costume after aya.


text:
Is Chic Innovation Hair Lounge incorrect or missing key business information? Update your Hotfrog listing today.
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Hotfrog Canada provides information regarding Chic Innovation Hair Lounge in Moose Jaw SK. Chic Innovation Hair Lounge is located at 32 Manitoba St W, Moose Jaw SK S6H 1P7 and provides personal care services. Contact them on (306) 691-2442.
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We have more personal care services in Moose Jaw SK available on Hotfrog Canada. You can update your search for Moose Jaw personal care by location, keyword or service options.
PO Box 2024 JctNorth Service Road & Hwy 2 North MOOSE JAW SASKATCHEWAN S6H 7N7
Action HONDA - 1325 Caribou St W MOOSE JAW SASKATCHEWAN S6H 7K5
24 Fairford St E MOOSE JAW SASKATCHEWAN S6H 0C7
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Clemson doctoral student produces rap album for dissertation; it goes viral
College of Education faculty member recognized by Council for Children with Behavioral Disorders
College of Education faculty member serves as co-editor of new book on black women and college success
Paying ClemsonForward: New research grants support growth
All The Difference - Charles H. Houston Center Film and Discussio...
11:00 am to 12:30 pm
Tillman Hall
A Day in The Life of an Education Major
8:45 am to 2:00 pm
Tillman Hall
Administration and Supervision Informational Session
5:30 pm to 7:00 pm
University Center of Greenville
Inaugural College of Education Research Forum
11:00 am to 1:00 pm
Hendrix Student Center
The College of Education is committed to being a transformative leader in improving education, beginning at birth. With a particular focus on serving underperforming schools and underserved communities, the school has award-winning programs that train teachers, counselors and school leaders; prepare counselors for community practice; train higher education student affairs leaders; and prepare training and development specialists for business and industry.
the number of undergraduate and graduate students enrolled in the school
the pass rate for teacher licensure


text:
Advanced Search | Shop by Brand
Leave a review
Ryobi RLT1830H13 ONE+ 18v & 240v Hybrid Grass Trimmer 250 - 300mm Cut with 1 Li-ion Battery 1.3ahWhy choose between corded & cordless power when you can get both in one tool? With the hybrid Ryobi RLT1830H13, you get all the benefits of unlimited run time when trimming close to a power source, & all the freedom of movement associated with a cordless tool. Cord not long enough? Just plug in the battery, & you're ready to go. In fact, adaptation is what the RLT1830H13 is all about; with a cutting width adjustable between 25cm & 30cm, & an EasyEdge head that can be set both for horizontal trimming & for vertical edging, there really isn't much that the trimmer can't handle. You can be sure of attractive results thanks to the RLT1830H13's comfortable & ergonomic Vertebrae design, for optimal control. The trimmer's automatic line feed keeps things simple, ensuring hassle free use.The long lasting 1.3Ah ONE+ Lithium Ion Battery included with this trimmer can be used to power any other Ryobi ONE+ tool, saving you unnecessary expense on batteries & chargers. Conveniently, the battery can be topped up in just 45 minutes. Features & Benefits:• Innovative 18V Hybrid power, offers the choice of seamlessly changing between corded and cordless power in the same tool• Powerful cutting with 1.6mm line and adjustable cutting width from 25-30cm for great versatility and run time optimization• Vertebrae design for improved comfort during use• EasyEdge feature allows the user to quickly transition between edging and trimming modes• Auto feed line advance for added convenience• Battery charges in 45 minutes getting you back to work fasterSpecifications:Battery pack capacity (Ah): 1.3Battery type: Li-ionCharger supplied: 45 minCutting line diameter (mm): 1.6Cutting path width [cm]: 25-30Cutting speed (rpm): 9000-8000Line feed system: Auto feedVoltage (V): 18Weight with battery pack (kg): 2.8Includes:1 Lithium Ion Battery 1.3ahCharger
Read all 5 customer reviews...
Richard | 15 September 2016
Very pleased with our purchase a little bit on the pricey side but worth it, a spare spool would nail it
Dave s | 26 September 2014
With the small li battery it needs more than one ideally although it can always be plugged in of course. slightly more powerful on mains anyway. With bigger garden the freedom of battery is really good - just plug in if you need to clear a well over-grown area perhaps.
Manjit | 30 July 2014
Only had the strimmer a few weeks but so far the feedback is all positive. it is light and easy to use (sufficiently for my 16 year old daughter to be chief strimmer) and does an excellent job in clearing quite densely overgrown area without spitting debris into your face. it takes about 45 mins to re-charge the battery so if you are clearing a large area it can be tedious. Overall this is a great compromise if you have a large garden and do not want to have to trail an electric cable over a distance or go industrial strength with a petrol strimmer.
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Tooled-Up.com78 Suez RoadBrimsdownEnfieldMiddlesexEN3 7PSTel : 0208 805 3535


text:
BonChon Chicken - Closed
New York, NY 10038-3201
- Daily 11am-12am
- Delivery, Takeout
- Reservations Accepted
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Kasadela Replaced by Boutique, Organika Will Take Over Candela Candela, and Bonchon on John is Gone for Now
Wednesday, February 6, 2013 at 2:48 p.m. by Jessica Goodman
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text:
MURDER victims' relatives yesterday blasted the decision to give jobs to three
women killers — all at the SAME Travelodge hotel.
Receptionist Pat Bulmer and guest room cleaners Linda White and Julie
Richardson are employed at the Travelodge as part of a prison scheme to
rehabilitate them.
Bulmer, 48, was jailed after she lured husband Robert into an ambush — then
watched as her lover KNIFED him to death in a plot to claim £32,500
White, 55, a heroin addict, repeatedly STABBED her partner in the face
and body after a row.
Richardson, 42, and two friends BURNED her dad with a hot iron, then
beat and kicked him to death.
Robert Bulmer's sister Hepsy yesterday said she was outraged by the
decision to hand the three murderers jobs.
She said: "It's disgusting. What are the authorities thinking of?
"I don't suppose the customers know a thing. If you're staying at a hotel you
don't expect to find three killers working there."
Another relative said: "I'm stunned." The trio, all jailed for life, applied
for jobs at the hotel in Piccadilly, York, while serving their sentences at
Askham Grange Prison on the outskirts of the city.
Their requests were granted under a scheme designed to help offenders who
present no threat return to society.
Bulmer and White have now been released on licence from Askham Grange and are
currently living in probation hostels.
Richardson is thought to have two years still to serve before she becomes
eligible for early release.
Travelodge Operations Director Jon Hendry-Pickup defended the decision to
support the scheme.
He added: "The workers from the rehabilitation programme are constantly
assessed and have proven to be dedicated and hard-working individuals.
"They are motivated to make a better life for themselves and should be given
every opportunity to succeed without prejudice.
"Travelodge does not discriminate and is a fair and progressive employer and
has supported a number of employment schemes."
The Ministry of Justice said: "Keeping the public safe is our priority.
Prisoners may be released to work only after they pass a rigorous risk
"Only those assessed as minimal risk of escape or risk of harm to the public
are eligible."


text:
More Videos...
Acura MD-X spied on camera
The vehicle will be manufactured at Honda's Lincoln plant in Alabama, USA and is expected to get a V6 3.5-liter engine for the entry-level variant. Various sources are indicating that the Japanese automaker will offer the 2014 MDX with an SH-AWD hybrid system for an additional cost. The power unit will probably be connected to a dual-clutch 7-speed gearbox. The design of the next generation model will be an evolution compared to the current model, featuring a prominent front grille, bigger air intakes and at a back a dual Exhaust system.
F1 race stars gameplay trailer
Today Codemasters® launched the first gameplay trailer for F1 RACE STARS, the fast-to-the-fun arcade racing game featuring official FORMULA ONE cars, teams and circuits as they've never been seen before. Set to arrive November 13 and available for pre-order now, the first trailer video is now playing at www.F1RaceStars.com.
GM's LT1 Engine Explained
GM Powertrain engineer John Rydzewski explains many of the features in the 2014 LT1 Corvette engine, including direct injection, continuous variable valve control, the rotating assembly, engine block, intake manifold, 11.5:1 compression ratio, and combustion system.
Chevy Corvette Stingray C7: Behind the Scenes of it's Design, Engineering & Build - Part One
MotoMan travels to Bowling Green, Kentucky & Detroit to learn how the Chevrolet Corvette C7 Stingray was created and meets Tadge Juechter, Chief Engineer, Tom Peters, Design Director, Dave Tatman, Plant Manager along with the rest of the people behind the return of the Stingray . . . MotoMan. Ordinary Car Guy. Extraordinary Adventures EVERY WEEK, Mean Gene & MotoMan bring you a new episode of their extraordinary adventures in the car world. Sure, MotoMan drives the latest cars to blue chip classics but that's just the beginning: He experiences bucket list car guy adventures like going behind the scenes to build manufacturer show cars to driving military prototype vehicles. Back at the studio, Mean Gene takes apart the newly introduced cars and provides his unique technical perspective. Subscribe now to experience our adventures in the car world. http://goo.gl/GjIHuZ Check out our Corvette playlist: http://goo.gl/IzRiiz Check out MotoMan's Playlist: http://goo.gl/79fTTj Come visit us at our home, The Petersen Automotive Museum on Museum Row in Los Angeles, California, where each month, MotoMan has an informal discussion with the key people shaping the car industry in front of a live studio & Internet streamed audience. Connect with us via Facebook & Twitter for a full schedule: Facebook - http://www.facebook.com/MotoManTV Twitter - http://twitter.com/MotoManTV Website - http://www.MotoMan.TV Petersen: http://www.petersen.org


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|DO NOT REPLY [Bug 49991] Tomcat 7 JAAS problem with CDI
|Tue, 19 Oct 2010 17:12:10 GMT
https://issues.apache.org/bugzilla/show_bug.cgi?id=49991 --- Comment #8 from Mark Thomas <email@example.com> 2010-10-19 13:12:05 EDT --- I've done a little digging on this. The best definition of the required behaviour is actually in the Javadoc: <quote> Interface for receiving notification events about requests coming into and going out of scope of a web application. A ServletRequest is defined as coming into scope of a web application when it is about to enter the first servlet or filter of the web application, and as going out of scope as it exits the last servlet or the first filter in the chain. </quote> Looking at Tomcat's current behaviour, it isn't firing the request listener for the request that displays the login page. That looks like a spec breach to me. I'll fix that and then see how the test case performs. If the test case is still broken then I'd lean towards the fixing this in Weld option. -- Configure bugmail: https://issues.apache.org/bugzilla/userprefs.cgi?tab=email ------- You are receiving this mail because: ------- You are the assignee for the bug. --------------------------------------------------------------------- To unsubscribe, e-mail: firstname.lastname@example.com For additional commands, e-mail: email@example.com
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It was January of 1848, and gold was discovered by James W. Marshall in Coloma California. By May of 1848, reports were stating "there is gold in them thar hills". Positive news about the gold were reported, and some 300,000 people arrived with dreams of getting rich. One of the ways that Samuel Brannan seized this opportunity was by opening a small store at John Sutter's Fort, right in the heart of the gold rush. Apparently he bought up all the picks, shovels and pans he could find and sold them at his store. According to reports, he would buy the pans for 20 cents each and then sell them for $15.
Samuel Brannan never found any significant amounts of gold, but selling shovels, picks and supplies to miners was one of the ways that made him California's first millionaire.
With the inevitability of rising fuel costs and stricter emissions standards for locomotives set to take effect in 2015, major railroad companies such as Berkshire Hathaway Inc.'s (NYSE:BRK.A) (NYSE:BRK.B) Burlington Northern Santa Fe LLC, Union Pacific Corp. (NYSE:UNP), Canadian National Railway (NYSE:CNI) and CSX Corp. (NYSE:CSX) are researching alternatives to manage the increases in expenses. An alternative that these companies are exploring is powering their locomotives using LNG instead of diesel.
The idea of LNG in the transportation industry is not a new one, as the trucking industry has been using LNG since the mid-'90s. Railroad companies such as Burlington Northern Railroad and Union Pacific also flirted with the idea in the late 1980s and 1990s, but the final engine technology was not completed, and the projects were shelved in the late 1990s, in part due to the rising costs of natural gas.
Motivated by the gap between the cost of diesel fuel and LNG, the idea of using this commodity has again sparked the interest of railroad companies. According to Bloomberg's Lorenzo Simonelli, chief executive officer of GE's (NYSE:GE) transportation unit, stated:
If you believe the price advantage over diesel is going to stay here for the next 10 to 15 years, then LNG is a revolutionary fuel.
Because of the potential savings and environmental impacts that the switch from diesel to LNG could produce, railroad companies, along with General Electric, Cummins Inc.(NYSE:CMI) and Westport Innovations (NASDAQ:WPRT), are currently exploring different avenues to capitalize on this potential shift.
In the same Bloomberg article, Tony Hatch, an independent transportation consultant based in New York, stated:
Like the move from steam to diesel, if it's going to be that radical a transformation, that's a lot of opportunity for sales in a lot of places.
One way to invest in the potential change in fuel is through Westport Innovations. Recently Westport teamed up with Canadian National railroad to provide the railroad company with four liquefied natural gas tenders. These tenders are designed to immediately help railroads validate the value of LNG in their operations today. These tenders offer a number of advantages that include: reducing the need for LNG refueling infrastructure and refueling stops. They are designed supply fuel to natural gas locomotives from virtually any manufacturer, and each tender can support two locomotives, reducing the capital investment required to move to LNG. Investors of Westport Innovations should be expecting positive revenue from the sale of these tenders to the "new market and off-road segment" of the business in the Q4 2013 reports.
Another company looking to take advantage of the potential fuel switch is General Electric. GE Transportation offers a retrofit kit that enables existing Evolution Series locomotives to operate on both diesel and LNG. These kits are designed to substitute up to 80% of diesel fuel with LNG. Even with the two sources, GE states its use can reduce fuel costs by 50% without diminishing their performance. These retrofit models currently meet US EPA Tier 3 emission standards and also allow a fully-loaded train to travel further distances without refueling stops.
Caterpillar (CAT) and General Electric are currently teaming up with Union Pacific to begin field testing liquid natural gas-fueled locomotives. As field testing has begun, Union Pacific indicates that they are still very much in the early analysis phase:
We are working closely with locomotive and engine manufacturers, cryogenic fuel-tank suppliers and natural gas/LNG suppliers to complete our analysis.
Final results from these tests are expected come fourth in at least one to two years, and according to railway magazine, if these tests are positive, "LNG locomotives could be in widespread use by 2016 or 2017."
As Railroad companies continue field testing LNG powered locomotives, researching companies that provide the "shovels" for this transformation should provide positive results for the investor. If you are looking for companies on the leading edge of this technology outside the railroad sector, Westport Innovations, Caterpillar Inc. and General Electric are three companies that provide products to the railroad companies that are the "shovels" in the transformation to LNG.
Disclosure: I am long CNI. I wrote this article myself, and it expresses my own opinions. I am not receiving compensation for it (other than from Seeking Alpha). I have no business relationship with any company whose stock is mentioned in this article.


text:
Parks Canada
Home > National Historic Sites > Lachine Canal National Historic Site > Activities
The Lachine Canal, a scenic 14.5 kilometre-long urban park, welcomes more than a million visitors on foot, by bicycle or aboard boats every year. Whether it's to admire the spectacular and diverse views of Montréal, to relax in a pastoral setting, or to indulge in sports activities, you'll experience a moment of pure escape in this tranquil natural setting.
Pilot Project – Winter Activities - New!
Starting in January 2017, the City of Montreal, in association with the Sud-Ouest borough and Parks Canada, is launching the second year of a pilot project of winter activities on the Lachine Canal. Come and enjoy the 6-km cross-country ski path and the 8-km winter trail. Fun times and rosy cheeks guaranteed!
Lovers of kayaking, pedal boats and small craft - go play in the water!
Watersport lovers can paddle the calm waters of the Lachine Canal while exploring part of Montréal's history. Come spend a Zen moment among the richness of the canal's contemporary and historical landscapes.
Sail on board the Petit Navire and enjoy a taste of romance and history
A green solution for navigating the Lachine Canal, the Petit Navire is a fabulous way to explore Montréal. Be carried away by the calm canal waters, discovering a route dotted with historic buildings and enchanting landscapes.
We've saved you a seat!
Since the summer of 2015, Parks Canada has installed a series of red Adirondack chairs at the Lachine Canal so that visitors can enjoy breathtaking views of Montréal and the rich historical heritage of this exceptional site. What are you waiting for to discover them?


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text:
Discover Gozo by 4x4
One of the best ways to discover the beautiful, hidden corners of Gozo is by 4x4 vehicle.
Dramatic photo opportunities abound as you journey through the hilltop villages of Qala, Nadur and Xaghra.
Cross the Ramla Valley and head up to Calypso's Cave to take in the panoramic view over Gozo's most beautiful beach.
Drive to Dwejra to see the Fungus Rock, the Inland Sea and the Azure Window. In this untouched part of the island, both time and nature seem to have stood still.
The road leads around the north side of the island, where you will see the dazzling mosaic formed by the saltpans.
Your 4x4 vehicle will stop in Victoria before returning you to the pier.


text: Master's Theses http://hdl.handle.net/2022/6469 2017-02-23T16:50:56Z 2017-02-23T16:50:56Z Characterizing Student Motivations in Outdoor Adventure Activities Zwart, Ryan http://hdl.handle.net/2022/21174 2017-01-05T08:00:13Z 2016-12-09T00:00:00Z Characterizing Student Motivations in Outdoor Adventure Activities Zwart, Ryan The purpose of this study was to develop a better sense of student motivations to participate in university outdoor recreation programs (ORPs) with the hope that this furthered understanding would grant new considerations for administration of these programs on college campuses. While the study did not find significant differences between the majority of courses, this researcher suggests that further research is necessary to determine effects that reduction of activity types would have on participant students, as well as, student trip leaders or long term members of ORPs. A thorough literature review of intrinsic and extrinsic motivations and insights from the Self-Determination Theory (SDT) provided a solid foundation for the development of this study. The SDT provides understanding in terms of the social development, trust, and control (Sibthorp & Jostad, 2014; Davidson, et.al, 2009) and how these outcomes and motivations encourage students to continue to take part in ORPs. The study clearly showed that these programs offer the social development and interpersonal relationships that students are looking for during their college years. 2016-12-09T00:00:00Z EFFECTS OF INTERLIMB TRANSFER IN LEARNING AND RETENTION OF SEQUENCES Kamadana, Prithvi http://hdl.handle.net/2022/21149 2016-12-17T08:01:23Z 2016-12-12T00:00:00Z EFFECTS OF INTERLIMB TRANSFER IN LEARNING AND RETENTION OF SEQUENCES Kamadana, Prithvi The current study investigated the effects of interlimb transfer in learning and retention of movement sequences in mirror and non-mirror conditions using a simple four element sequence learning task. A total of Forty eight healthy, Right Handed subjects were recruited for the study. The subjects are required to perform a few key presses following a stimulus on a color monitor using a standard numerical keyboard. Four conditions were tested which include 1) right hand practice with mirror 2)right hand practice with non-mirror, 3) left hand practice with mirror, and 4) left hand practice with non-mirror. The reaction time (RT), movement time (MT), total time (TT), were measured using E-prime software program. Two way ANOVA was conducted to see the main effects and interaction effects of practice, retention and transfer for mirror and non-mirror conditions. The two-way ANOVA conducted on MT measures showed significant interaction effects. The practice Hand x Retention Test indicated for the left mirror condition, practicing with the left hand was significantly slower than practicing with the right hand, whereas for non-mirror retention condition, there is no significant difference in practicing either with the right or left hand. The Retention Test x Practice Hand interaction is also significant, for the left hand practice condition, mirror was slower when compared to non-mirror, whereas for the right hand practice condition, non-mirror was slower compared to mirror. Our results indicate differences in motor code execution while processing mirror vs. non–mirror condition with right and left hands. 2016-12-12T00:00:00Z EFFECT OF GRASTON TECHNIQUE ON EDEMA FOLLOWING A SPRAIN TO THE LATERAL ANKLE LIGAMENTS Thaman, Noah http://hdl.handle.net/2022/20916 2016-11-04T07:01:52Z 2016-05-01T00:00:00Z EFFECT OF GRASTON TECHNIQUE ON EDEMA FOLLOWING A SPRAIN TO THE LATERAL ANKLE LIGAMENTS Thaman, Noah STATEMENT OF THE PROBLEM: Lateral ankle sprains are one of the most common orthopedic injuries experienced by active individuals. Deleterious effects of swelling within the ankle joint include pain and reduced functionality resulting in complications with sport and daily living activities. Treatment techniques resulting in rapid reduction of edema offer significant benefits to patients by reducing pain and increasing function. The Graston Technique, a form of soft tissue mobilization, presents a novel approach in edema reduction and requires further study. PURPOSE: To evaluate the effectiveness of Graston Technique (GT) on reducing edema following an acute sprain to the ankle ligaments. METHODS: 16 participants were recruited for this study. Subjects were drawn from the recreational athletic population of a large Midwestern university town and were randomly assigned to one of two groups. The GT group was treated with a single session of the Graston Technique while the control group received no treatment. Ankle girth was measured at study enrollment, 10 minutes post-treatment, and after 24 hours using the figure-of-8 method. Furthermore, subjective representations of pain and function were gathered using a Visual Analog Scale (VAS) and the Foot and Ankle Ability Measure (FAAM) respectively. A repeated measures analysis of variance was completed to assess differences between groups. RESULTS: Results of the statistical analysis revealed no significant difference in reduction of ankle girth between the Graston Technique and control groups. While participants experienced decreases in girth as measured pre-test and at the 24 hour post-test, these decreases remained similar regardless of group allocation. Furthermore, assessment of subjective function and pain as measured by FAAM and VAS respectively revealed no significant difference between groups. CONSLUSIONS: Current testing does not indicate that the Graston Technique is more effective than conservative management at altering ankle edema following an ankle sprain. These data indicate that a single session of GT treatment is similar in its efficacy to non-intervention at reducing swelling, decreasing pain, and increasing function. 2016-05-01T00:00:00Z EXTENDED EFFECTS OF PARTICIPATION IN A SEMESTER-LONG OUTDOOR LEADERSHIP TRAINING PROGRAM Staley, Molly http://hdl.handle.net/2022/20886 2016-11-04T07:02:16Z 2015-06-01T00:00:00Z EXTENDED EFFECTS OF PARTICIPATION IN A SEMESTER-LONG OUTDOOR LEADERSHIP TRAINING PROGRAM Staley, Molly While many adventure programs offer opportunities to develop technical and outdoor skills such as orienteering, rope management, and backpacking skills (Shooter, Sibthorp, & Paisley, 2009) research has demonstrated that participating in an adventure program often results in the development of interpersonal skills such as communication, decision-making, and problem solving (McKenzie, 2003; Raiola, 2003; Sibthorp, Paisley, & Gookin, 2007). Interpersonal skills are beneficial to both personal and professional development and can translate into success in the classroom and the workplace (Gass, Garvey, & Sugerman, 2003; Shooter, Sibthorp, & Paisley, 2009). However, the long-term retention of technical and interpersonal skills as a result of participating in an adventure program has received limited research attention. The purpose of this study was to examine the effects of participation in the CORE program at IU through a longitudinal analysis. Specifically, the aim of this study was to analyze the retention of technical and interpersonal skills, and attitudes of past CORE program participants and identify the impact these skills have on areas such as personal and professional development. The CORE Program is a unique outdoor adventure and education program that blends classroom learning and fieldwork to provide its participants with a broad range of experiential learning opportunities. The past research done on the CORE program has typically examined the effects of the program immediately after its conclusion. This study adopted a self-administered retrospective pre-post questionnaire that asked CORE alumni to reflect and rate their skill level on a 5-point Likert scale on three time points: before participation in CORE, right after completion of the CORE program and their current level of skills. Skills examined included technical skills such as knots, climbing, and orienteering and interpersonal skills such as problem solving, group management, and decision-making. The results of this study contributed to a better understanding the impacts and outcomes of the CORE program. 2015-06-01T00:00:00Z


text:
Showing 1 - 1 items
Reg. $1,199.00
The Kastle BMX 78 makes a great ski for the strong intermediate to expert skier who is seeking smooth and easy turns on the front side of the mountain with a very quick edge to edge feeling. A 78mm waist is very agile and nimble for zipping quicker ti... Read More
{"boxId":"compare31f45b4842e77234b6155fbd19", "id":"426415P"}


text:
Invaluable cannot guarantee the accuracy of translations through Google Translate and disclaims any responsibility for inaccurate translations.
20th Century Decorative Arts (374 Lots)
by Semley Auctioneers
374 lots | 191 with images
June 15, 2013
Near Shaftesbury, United Kingdom
Description: Property of a lady - an HM silver jam spoon and matching mustard spoon, with duck head finials, by Sarah Jones, London 1983 and 1987, the first 4.5ins. (11.5cms.) long (2).View additional info
Description: Property of a lady - a small jewellery box containing assorted costume jewellery & a set of six Arts & Crafts hammered pewter & coloured enamel circular buttons; together with an HM silver topped glass dressing table jar (2).View additional info
Estimate: Log in or create account to view price data
Description: Property of a gentleman - a group of six Holmegaard glass items, four variously dated 1952-1958, the tallest 4.3ins. (11cms.) high (6) (see illustration).View additional info
Description: Eight coloured glass items including two Vasart signed examples (8).View additional info
Description: Six Art Nouveau glass items including Loetz style iridescent examples (6).View additional info


text:
Something Meaningful | Photography by Brittany Evans
Portrait Photography, Professional Photographer, Portrait Photographer
Something Meaningful is a boutique photography service based in Melbourne Australia with a strong focus on Fine Art Newborn and Baby Photography. Brittany Evans is a professional photographer, with many years experience in newborn photography & baby photography. The name Something Meaningful stands for her passion of capturing memorable moments.
Brittany has a special gift for photographing newborns & babies, she is dedicated to capturing those precious moments of your baby's early life, following baby's lead to capture them, safely and beautifully. She has a very soothing & patient approach to her sessions. Brittany's style has been described as contemporary, clean & simple. Her aim is to create beautifully unique art of your newborn or baby that you & your family will love and cherish for a lifetime.
Examples of Brittany's work can be viewed in newborn, baby, & maternity galleries on Something Meaningful's website & Facebook page.
The Something Meaningful studio is conveniently located just 20 minutes west of the CBD of Melbourne. Almost all sessions take place in Brittany's natural light studio with clients travelling to her from all over Melbourne & Victoria. On location sessions are also available, these sessions are quite unique as Brittany comes to you to capture your little ones first moments in the comfort of your own home.
If you are looking for a passionate and experienced photographer, contact Brittany via the website.
Print this page
Something Meaningful is a boutique photography service based in Melbourne Australia with a strong focus on Fine Art Newborn and
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Message: A new issue has been created in JIRA. --------------------------------------------------------------------- View the issue: http://issues.cocoondev.org/jira//secure/ViewIssue.jspa?key=FOR-260 Here is an overview of the issue: --------------------------------------------------------------------- Key: FOR-260 Summary: put a little error icon behind broken links Type: Improvement Status: Unassigned Priority: Minor Project: Forrest Component: Core operations Versions: HEAD Assignee: Reporter: Johannes Schaefer Created: Thu, 19 Aug 2004 11:45 AM Updated: Thu, 19 Aug 2004 11:45 AM Environment: all Description: an icon (like the external link icon) indicating that this link is broken (error:site:...) would be very helpful in spotting broken links --------------------------------------------------------------------- JIRA INFORMATION: This message is automatically generated by JIRA. If you think it was sent incorrectly contact one of the administrators: http://issues.cocoondev.org/jira//Administrators.jspa If you want more information on JIRA, or have a bug to report see: http://www.atlassian.com/software/jira
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text:
For Everyone.
Get bookings
List your place
My Favourites
South Africa
Limpopo Province
Waterberg Biosphere Reserve
Bushman's Hide
All Comments for
Bushman's Hide
Looking for the recharge button?
Mervin Wilkinson, South Africa
25 September 2013
An isolated escape from the hustle of everyday life. Completely remote, Bushman's offers a complete recharge. I'd recommend it to anyone who needs to get away from it all. We felt completely rested after the long weekend. The host family is warm and generous and the tents are luxurious and adequately equipped. The food is delicious and abundant.
Anesia Bester, South Africa
06 May 2013
Very beautiful and stowed away in the nicest surroundings you can imagine. Staff is professional and courteous. Neat and tidy. Food is fantastic and in abundance.
Hidden gem
Anonymous, South Africa
26 September 2010
What an amazing place. My wife and I enjoyed four nights at Bushman's Hide. The website photos simply do not do justice to this beautiful and secluded location. A beautiful reception and dining room is reached through a winding entrance between the rocks, with a high thatched roof and exquisite decor. A spectacular deck area complete with pool and loungers, leading to the observation platform built on the highest point of the rocks overlooking the water hole where zebra, nyala, Cape Eland, warthogs and baboons regularly congregate. The observation deck is thatched and furnished with easy chairs for comfortable nature watching and a second dining table where we enjoyed fabulous meals cooked by Stef. He (and Henrick and Lisbeth) looked after us amazingly- nothing was too much trouble. The tent was superbly equipped and extremely comfortable. As well as the food and accommodation, other highlights were the bush walks around the camp which we took every day, the quad bike tour and the game drive on the neighbouring reserve. We were the only couple staying so we had everything to ourselves. Hard to believe Bushman's Hide is not permanently booked out. Thoroughly recommended for the peace and quiet, immaculate facilities, fabulous service and closeness to nature.
Bushman's Hide
Anonymous, South Africa
12 August 2010
Perfect place to go to for a weekend. Service was excellent and we enjoyed the stay. No cellphone reception was an added bonus. We will visit them again. Thank you for the great service.
Bushman's Hide
Anonymous, South Africa
21 September 2009
What a wonderful week end! Bushman's Hide is a real treat. It is a small, exclusive camp in the heart of the Waterberg Mountains without a sign of civilisation as far as the eye can see. The tented accommodation is very private and the decor beautiful! The camp itself is unique in that it is built in amongst huge rocks and boulders which adds to the bush experience and the views are spectacular. Our hosts were friendly and gracious. As for the cuisine ....hats of to the chef!! Bushman's Hide really does offer an exclusive escape for that special getaway.
Bushman's Hide
Fall in love at Bushman's Hide, the heart of the Waterberg.
Hidden in the Waterberg biosphere nature reserve, Bushman's Hide is a welcoming vision of understated luxury amid the archaeological treasures and biological diversity of this beautiful wilderness.
With just four luxury safari tents – each with a queen bed, bathroom, bar fridge, coffee station, private patio and outdoor fireplace – Bushman's Hide offers a special tranquillity and romance.
Enjoy three delicious meals daily – served on your private patio, at the poolside, beside the fire, on the lookout deck, or packed into a picnic basket.
Activities include nature walks, easy quad biking trails, visits to the Heuser family's beloved Nguni herd, photography retreats, wine tasting weekends and more.
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*This item is on display in our showroom, however fabrics or color in the picture may vary from
actual product in the store. Please stop in or click Request More Info for additional details.
Why not show our Low Price?Rotmans sets our own prices on our TV & Electronics items, however some manufacturers have restrictions on how we may display the prices of their products. Since our price is frequently lower than their suggested retail price, they may not allow us to show you our Low Price until you place the item in your shopping cart. Adding this item to your cart does not require you to purchase it & you can easily remove it from your cart should you decide not to purchase.
Update your living room or family area with this chic and comfortable loveseat. The piece features flare-tapered arms with subtle rouching and classic turned legs for a sophisticated appearance. Clean lines add contemporary appeal, creating a versatile transitional look. Plush reversible cushions and two included accent pillows ensure comfort as you entertain friends or watch TV with your family.
The Pemberley Transitional Loveseat with Turned Legs by Serta Upholstery from Rotmans. We proudly serve the Worcester, Boston, MA, Providence, RI, and New England area. Product availability may vary. Contact us for the most current availability on this product.
Elevate your living space with the sophisticated transitional designs from this seating group. Pieces are designed with flare-tapered arms and box edge seats for a contemporary silhouette. Details like rouching and turned legs give the sofa and loveseat a transitional look that exudes relaxed refinement. Plush reversible cushions and coordinating pillows bring comfort to each seating option.
The Pemberley collection is a great option if you are looking for Transitional furniture in the Worcester, Boston, MA, Providence, RI, and New England area.
These images are representative of this group as a whole and may differ from this item.
Browse other items in the Pemberley collection from Rotmans in the Worcester, Boston, MA, Providence, RI, and New England area.
This item has been tagged as: Loveseat, Love Seats, Reclining Love Seat, Sofa Love Seat, Loveseat Recliner, Love Seat Sleepers, Loveseat Glider, Small Love Seat, Love Seat Hide-A-Bed, Love Seat Chaise, Modular Sectional Pieces, Couch, Small Couch, Sofa, Small Sofa, Settee, Divan, Davenport, Couches, Seating for Two, Double Chair, Settee for Two People
Nothing on this page may be copied or reproduced without explicit permission.
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If you are still having trouble logging on after receiving your new password, please email firstname.lastname@example.com


text:
Jun 13, 2014From Buyer: kclarksville
Promt communication and the item was arrived exactly as it was described in the ad. Great person to deal with. Thank you
Item: Sennheiser HD650
Jun 10, 2014From Buyer: snapple10
Grado PS 500- Immediate responses. Shipped earlier than expected. Condition as described and great seller. Tx
Item: Grado PS-500
May 27, 2012From Buyer: punkplayer
I bought the dt990 from JohnnyBlaze554. The transaction is very smooth, he posted the headphone very quickly! Very reliable head-fier!
May 14, 2012From Buyer: Night Crawler
Great seller! The FiiO E7 & E9 shipped out really fast, and the amps were carefully packaged as well.
Item: FiiO E7 and E9 combo


text:
The overall cellular response to ionizing radiation exposure is not limited to cells directly irradiated, but includes neighboring "bystanders". Essentially every endpoint used routinely to test for changes in cells directly exposed to radiation, such as mutation, apoptosis, micronuclei formation or cell cycle checkpoints, has been studied in bystanders. This phenomenon has caused a paradigm shift in thinking about biological effects and associated health risks of radiafion exposure;the impacted cell population is larger than once believed. The mechanism regulating bystander effects is not well understood. However, certain gap junction proteins, i.e., Connexin 32, and proteins like Cox-2 control the overall process, whereas p21 just regulates the radiation-induced Gl to S checkpoint. We have shown that Rad9 regulates the bystander response to radiation, since Rad9 null enhances bystander apoptosis and micronuclei formation. Rad9 protein, which controls cell cycle checkpoints, DNA repair, apoptosis and transactivation of downstream target genes in directly irradiated cells, likely impacts on the bystander response via more than one pathway. In collaboration with investigators in Projects 2 and 3, we show that Rad9 is essential for bystander induction of Cox-2 and p21 (Project 2), and production of Connexin 32 in HeLa cells, which are normally devoid of the protein, increases bystander radiation resistance and levels of Rad9 as well as TCTP (Project 3), which both function in the bystander process. We also show that TCTP and Rad9 physically interact (Project 3). Our overarching hypothesis is that Rad9 plays an important role in radiation-induced bystander effects via mechanisms involving at least Cox-2, p2I, Connexin 32 and TCTP.
Specific aims to address this hypothesis are: I. Determine the activity of Rad9 in nuclear versus cytoplasmic irradiation-induced bystander effects;2. Analyze Rad9 functional domains and protein-protein interactions for roles in the bystander process;3. Establish the significance of Rad9-dependent bystander induction of Cox-2, p21 and other genes for bystander regulation;4. Determine if Rad9 is critical for bystander mutagenesis;and 5. Define the significance of junctional communication on the regulation and function of Rad9. These studies will reveal mechanisms by which Rad9 regulates radiation-induced bystander effects, and will impact on understanding the biological response to radiation exposure, with implications for improving radiotherapy and better defining health risk.
): It is now established that the effects of radiation exposure extend well beyond directly targeted cells or tissues, and involve neighboring bystanders. The molecular basis for this paradigm shift in thinking is not well understood, and is the focus of this proposal. Understanding the bystander response will impact on radiotherapy and health risk assessment, where radiation exposure is a crucial component.
|Zhang, Bo; Davidson, Mercy M; Hei, Tom K (2014) Mitochondria regulate DNA damage and genomic instability induced by high LET radiation. Life Sci Space Res (Amst) 1:80-88
|Broustas, Constantinos G; Lieberman, Howard B (2014) DNA damage response genes and the development of cancer metastasis. Radiat Res 181:111-30
|Luo, Xiuquan; Suzuki, Masatoshi; Ghandhi, Shanaz A et al. (2014) ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS One 9:e99983
|Broustas, Constantinos G; Lieberman, Howard B (2014) RAD9 enhances radioresistance of human prostate cancer cells through regulation of ITGB1 protein levels. Prostate 74:1359-70
|Ivanov, Vladimir N; Hei, Tom K (2014) Radiation-induced glioblastoma signaling cascade regulates viability, apoptosis and differentiation of neural stem cells (NSC). Apoptosis 19:1736-54
|Li, Min; Gonon, Geraldine; Buonanno, Manuela et al. (2014) Health risks of space exploration: targeted and nontargeted oxidative injury by high-charge and high-energy particles. Antioxid Redox Signal 20:1501-23
|Zhao, Y; de Toledo, S M; Hu, G et al. (2014) Connexins and cyclooxygenase-2 crosstalk in the expression of radiation-induced bystander effects. Br J Cancer 111:125-31
|Shim, Grace; Ricoul, Michelle; Hempel, William M et al. (2014) Crosstalk between telomere maintenance and radiation effects: A key player in the process of radiation-induced carcinogenesis. Mutat Res Rev Mutat Res :
|Ghandhi, Shanaz A; Ponnaiya, Brian; Panigrahi, Sunil K et al. (2014) RAD9 deficiency enhances radiation induced bystander DNA damage and transcriptomal response. Radiat Oncol 9:206
|Ivanov, Vladimir N; Hei, Tom K (2014) A role for TRAIL/TRAIL-R2 in radiation-induced apoptosis and radiation-induced bystander response of human neural stem cells. Apoptosis 19:399-413
Showing the most recent 10 out of 185 publications


text:
Trote Solidário e Ecológico 2012
Guarujá, Brazil
Our 29th voluntary action will be organized especially for the new students who joined the in Marine Biology course at the Universidade Santa Cecilia - UNISANTA. Six dives will be drawn for the new students who have diver credential. As a whole will be 10 divers divided into three teams. The rest of the students will make through snorkeling and clean the beach.


text:
We use plastic all the time, have been for years. We used to use sheets,
but they would not allow the light through, and they would become wet, were too
small at times, were unsightly, and blow off, etc. We have found that using
plastic over plants that are in the ground, in pots on the ground, or plants
near the house really works well. The key is not to let the plastic lay
ontop of the plants. We use large sheets that we secure to the gutter and hang
down and have bricks on to protect the plants near the house. Most of the
plants (especially tomatoes are in cages) have poles or stakes for support or
such, which the plastic can easily drape over the top without touching the
plants. Even where the plants are touched on the sides, the plastic doesn't
hurt it. We use the clear plastic obtained at Home Depot/Lowes. It comes in
different mil thick, and we reuse it for years. You can get 10 to 20 foot
We put the plastic up when we hear of a freeze coming......we do it before a
freeze...that way the ground and the sun warm up the area within the plastic,
and maintain that warmth. It works exactly like a hothouse or greenhouse
would. Same material. The key is to vent it when it gets warm again. I
have yet to lose a plant to the plastic touching the plant or such, but I have
lost a plant to cooking it to death by not venting the plastic the next day
after a freeze when the temps warmed up again. If it is only one night, you
can take it off again....if more nights, vent the plastic if the temps go up
quite a bit. Here we have dips into freezing at night sometimes, but the day
time temps will be in the 70's. If the temps stay cold day and night, keep it
No freeze forecast for here, although temps will dip down in the 40's that
zone 9
Texas Gulf Coast
In a message dated 4/13/2008 3:10:52 PM Central Daylight Time,
email@example.com writes:
Can you throw sheets and blankets over things?
I see people put plastic but I have read that plastic is not good for
protection. What do you think
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Home Base Work-Online Tourism Promotion ( 0 - 1 yrs)
Forever Success Club
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This guide provides important information to help you make the right decision. Learn about the different types of readers/detectors; read modes, applications, technologies and other important considerations.
Microplate readers are used to detect biological, chemical or physical events in microtiter plates via the measurement of light. Microplate readers/detectors are the key workhorses in many laboratories and are extensively used for many applications across a wide range of disciplines including life sciences, basic research, target discovery, lead discovery, bioassay validation, quality control, drug safety, toxicity testing and biopharmaceutical/pharmaceutical manufacturing processes.
Although a well established product category, microplate readers are continuing to evolve towards greater functionality, flexibility, speed and throughput. Currently there is a wide variety of microplate readers on the market, offering different capabilities and functionalities.
Microplate readers differ by the type of detection mode. The most common detection modes are absorbance, fluorescence and luminescence. Additional detection modes include fluorescence resonance energy transfer (FRET), time-resolved fluorescence (TRF), fluorescence polarization, bioluminescence resonance energy transfer (BRET), AlphaScreen and nephelometry.
Microplate readers also differ by detector technology. Light detection can be performed using filters, monochromators or spectrophotometry. Monochromator based technology has the most flexibility in wavelength detection, so is most suitable for labs with variable detection requirements. Newer spectrometer-based readers can capture an entire spectrum of wavelengths, offering even more flexibility in detection. Filters are for fixed wavelength detection but offer greater sensitivity, so are more appropriate for labs with limited detection needs. Hybrid systems combining these technologies are also available. In addition, lasers rather than flash lamps are better for time-resolved fluorescence and AlphaScreen detection.
Tip: When considering the number of read modes required multi-mode readers provide greater flexibility but if your current and future requirements are limited, it may be more cost-effective to purchase a reader dedicated to your main application.
Microplate readers can be purchased as either single-mode or multimode; the latter combines several read modes in one instrument. Multimode microplate readers enable researchers to perform multiple assay types in one system. Multimode readers are typically more expensive than single-mode readers but purchasing one multimode reader is likely to be more cost-effective than buying several dedicated machines. In addition, they do not require much more bench space than a single reader and are often modular and upgradable, allowing a laboratory to purchase only what they need at the time but allowing for future additions.
For example, the PHERAstar FSX®, (see Figure 1) from BMG LABTECH is a multimode reader, which now includes a Simultaneous Dual Emission detection system that allows two wavelengths to be read at the same time. The sensitivity of the reader, combined with faster processing times make it perfect for more sophisticated applications, including FRET, TR-FRET and BRET assays. This modular reader is also compatible with a number of accessories designed to enhance plate reading workflow, including stackers and incubators. Watch the video (Figure 1) to hear Senior Scientist, Helen Harrison, describe how the PHERAstar FSX® has accelerated workflow at Bicycle Therapeutics Ltd.
Figure 1: The PHERAstar FSX® from BMG Labtech
When choosing a microplate reader/detector the first, and perhaps the most important, considerations are the application requirements. Microplate readers are used across diverse scientific disciplines for varying applications, the most common methods are briefly introduced below.
Common ApplicationsEnzyme-Linked Immunosorbent Assay (ELISA)
ELISA is an immunodetection assay that uses labelled secondary antibodies to detect specific antigens. The secondary antibody conjugate, such as horseradish peroxidase (HRP), is detected via an enzyme-mediated chromogenic change. ELISAs are used extensively in the life sciences, pre-clinical research and clinical diagnostics. The majority of plate readers are able to read ELISAs.Nucleic Acid Quantification
DNA and RNA absorb light within the UV range. Absorbance of a sample at 260 nm can be used to calculate the concentration of double-stranded DNA, single-stranded DNA or single-stranded RNA. Protein contamination, common in DNA and RNA preparations, can be determined using the 260/280 nm absorbance ratio. Plate readers with UV bandwidth detection capabilities are suitable for this detection.Gene Expression
Reporter genes, such as luciferase or GFP can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteration. Any reader with appropriate wavelength detectors can be used for this detection.Enzyme Kinetics
Measurement of enzyme activity is a common assay in life sciences and pre-clinical research. The time-resolved, enzyme-dependent accumulation of a marker or product can be used to determine enzyme kinetics. The majority of plate readers have this capability.Basic Protein Assays
Protein quantitation is another common application. As discussed above, protein concentration can be determined by absorbance at 280 nm, however, many chromogenic reagent-based assays have also been developed. Microplate readers can be used to measure total protein concentrations using a variety of commercially available kits. Watch the video (Figure 2) to discover how the SpectraMax®i3x Multi-Mode Microplate Reader from Molecular Devices® can enhance protein assays, with optional upgrades that enable Western blotting and imaging.
Figure 2: The SpectraMax®i3x Multi-Mode Microplate Reader, from Molecular DevicesFluorescent Protein Assays
Fluorescent markers such as EGFP, YFP, mCherry and mTomato can be used to measure a variety of real-time cell-based activities, including: intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and translation. These assays are often applied to live cells, necessitating a microplate reader with appropriate environmental controls. BMG LABTECH's CLARIOstar® comes with oxygen and carbon dioxide controls to support live cell assays (see Figure 3).Pharmacology Assays
Fluorescent or luminescent markers are also used in pharmacological and drug discovery assays. These are used to investigate ligand binding and protein interactions. Read about a novel nanoBRET assay developed in association with BMG LABTECH and watch the video (see Figure 3) to hear Dr Kevin Pfleger, Associate Professor, University of Western Australia, describe how BMG LABTECH's CLARIOstar® and PHERAstar FSX® were instrumental in its development.
Figure 3: The CLARIOstar®, from BMG LABTECHCell Density & Counting
Bacterial cell counting can be determined by optical density at 600 nm, which indicates the growth phase for harvesting the cells. For other cell counting applications, watch the video (Figure 4) to discover how the Spark™ 10M Multimode Microplate Reader, from Tecan, can optimize cell incubation, counting and dispensing functions for long-term analysis.
Figure 4: The Spark™ 10M Multimode Microplate Reader, from TecanCell Viability, Proliferation and Cell Death
There are many different commercially available cell based assay kits that enable cell viability, proliferation and apoptosis to be measured. These include those for monitoring ATP, measuring caspase activity and detecting bromodeoxyuridine (BrdU). They are also used in toxicity testing in pre-clinical drug development. TTP Labtech's acumen® Cellista Laser Scanning Imaging Cytometer allows whole wells to be scanned and fluorescent colonies to be counted, as well as determining sizes of colonies, find out more about theses cell health assays in this video .
Figure 5: The Cellista Laser Scanning Imaging Cytometer, from TTP LabtechHigh Content Screening
Drug discovery & manufacturing applications often require high content screening (HCS) and/or high throughput screening (HTS). Most of the applications above can be conducted in high content, multi-well plates in microplate readers. High throughput drug screening for target validation and ADMET (absorption, distribution, metabolism, elimination and toxicity) is a key process in drug discovery. However, phenotypic cell-based assays are now becoming more commonplace. In addition to conventional assays, these readers enable phenotypic screening of cells, either by well or per cell. Multimode microplate readers have been designed specifically for this HTS combined with imaging. Watch the video (Figure 6) to discover how the EnSight™ Multimode Plate Reader , from PerkinElmer, enables fast visualization and generation of per-cell or per-well data, to enhance the speed of high content cell toxicity assays.
Figure 6: The EnSight™ Multimode Plate Reader, from Perkin Elmer
Tip: The ability to measure all wells of a microplate at once is a factor to consider alongside reliability, sensitivity and consistency, which are essential for high throughput screens.
Phenotypic screening is used routinely in drug discovery for the identification of substances that alter the phenotype of a cell or an organism. The target cells are screened with compounds or biopharmaceuticals to assess modulation of the activity of interest. In vitro phenotypic screening uses cell lines and cell based assays and is often performed in high content microplates, and may utilize live cells. Watch the video (Figure 7) to find out how the Corning Epic® System is enabling high throughput assay miniaturization at AstraZeneca from Senior Scientist, Helen Plant.
Figure 7: The The Epic® System, from Corning at AstraZenecaLive-Cell Assays
In live, real-time cell based experiments, it is beneficial to read from the bottom of the microplate and rather than the top. Bottom reading offers several advantages for cell based detection: the light collector can be placed closer to the sample and the cell layer adherent closer to the bottom of the well, which decreases light dissipation. Moreover, the interfering effect of the cell culture medium is significantly reduced. Both factors positively affect sensitivity. In addition, bottom reading allows for a cover or lid to be placed on top of the microplate to prevent cell contamination and liquid evaporation. This is particularly important in time-lapse experiments. Several readers are optimized for live cell assays, including BMG LABTECH's PHERAstar FSX® (see Figure 1) and CLARIOstar® (see Figure 3) readers, which use a proprietary Direct Optic Reading system to eliminate the need for fiber optics. BioTek's new Synergy™ Neo2 features increased speed and sensitivity for live cell assays, find out more in the video (figure 8).
Figure 8: The Synergy™ Neo2, from BioTek
Tip: Consider whether you need your microplate reader for real time, live-cell assays and which technologies or modules are available and suitable for your applications.
Recently, there has been increased drive to develop assays and readers for 3D cell cultures. These spheroids or cell clumps can be grown in multi-well plates and more closely mimic the endogenous environment, which is important in both life sciences and drug discovery applications. Watch the video (Figure 9) to hear how the accuracy and reliability of BioTek's Cytation 5 Cell Imaging Multi-Mode Reader is enhancing 3D cell culture drug screening at Likarda, from President and CEO, Dr Lisa Stehno-Bittel.
Figure 9: The Cytation 5 Cell Imaging Multi-Mode Reader, from BioTek at LikardaAmplified Luminescent Proximity Homogeneous Assay Screen (AlphaScreen)
AlphaScreen is a versatile assay technology developed to measure analytes using a homogenous protocol that enables sensitive and precise interrogation of signaling pathways, receptors, enzymes and kinase targets, in a cell based format. AlphaScreen has been particularly useful for screening GPCRs, growth factor receptors, intracellular MAPK inhibitors and many other signaling pathways.Homogeneous Time Resolved Fluorescence (HTRF)
Utilizing rare-earth lanthanides with long emission half-lives as donor fluorophores, HTRF technology combines standard FRET with the time resolved measurement (TR) of fluorescence. HTRF is commonly used for GPCR and kinase screening, two of the most important target classes investigated within drug discovery. Other HTRF applications include discovery of new biomarkers, studies of protein-protein interactions, epigenetics and an alternative method for bioprocess monitoring.
Microplate readers/detectors are used for numerous applications and as technologies evolve, even more applications will emerge. When purchasing a microplate reader/detector, it is important to establish what applications you will be using it for now, and in the future. You will want to ensure that your chosen microplate reader/detector is capable of fulfilling all your requirements but also consider how easily upgradable it is for your future needs.
Try it Out: Once you have considered both your application needs and the different technologies that are available, the chances are you will have narrowed down the choice to just a few microplate readers. Before going ahead and purchasing a microplate reader/detector, it is highly recommended that you try it out. Most manufacturers will let you demo the instrument for a trial period. This is a great opportunity to ensure the reader fulfills all your requirements, is easy to use and to make sure you get the right support and training to use the instrument.
Technical Support and Warranty: Consider the extent of the technical support and training that is available from the manufacturer at the point on purchase, is it included in the purchase cost? It is also recommended that you look into the company's policy on after-sales service and standard warranty. While everything is operating well, this may be the last thing on your mind, but in the event that after sales support is required, it is important to know how accessible that is.
Accessories and Add-Ons: Not all microplate readers have the same standard features. Make sure to ask what is included with the reader at the time of demonstration. Optional items may be key parts of the kit including: computers to run the instrument, filters or filter cubes, software licenses, software upgrades, FDA compliant software, environmental controls or injectors.
Software: Microplate readers take hundreds or thousands of measurements, so data acquisition and analysis are key to efficient workflow. Make sure that you know how to use the software for your current needs and how to keep it updated, find out how well it is supported by the manufacturer.
Flexibility: Many labs have equipment from numerous different manufacturers. Consider how well your chosen reader will integrate with your existing equipment and how easy it will be to upgrade in the future. Many models have modular design and add-ons but their compatibility may be brand limited.
Future Needs: Taking into consideration current and future trends is essential in maximizing the lifespan of the microplate reader/detector. As systems become more sophisticated, integration, convenience and application specificity will be fundamental. Systems will become faster and more efficient and be able to run a larger variety of different assays. Application possibilities will increase as manufacturers work to provide solutions to individual and industry demands. Manufacturers are taking customer feedback and requirements into consideration, with many developing strategies for even greater specificity assays and technology for the future.
Whether purchasing a microplate reader/detector for a new application, or replacing an existing system, there are a number of factors to consider. You will need to examine your current and future application needs and determine which of the available technologies best suits these applications.
Visit the SelectScience Microplate Readers / Detectors product and user product reviews directory for an overview of the latest products from leading manufacturers. Keep up-to-date with the latest techniques and advances in Microplate Reader technology by visiting the SelectScience Microplate Readers / Detectors pages for application notes, videos and the latest news.
"Buying for the third time clearly shows how impressed we are with their performance"
Puneet Khurana, Medical Research Council Technology (MRCT)
"Excellent and innovative product. Highly recommended."
Kristjan Plaetzer, University of Salzburg
"Easy to use and protocol addition is very simple. Readings are rapid but reproducible."
Sarah Short, Vanderbilt Medical Center
"Flexible platform that enables a wide range of fluorescence assays."
Sean Johnston, Harvard Medical School
"Very good instrument, we will most likely purchase more."
Adam Johnson, Centers for Disease Control


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Open All Year
Reserve: 800-562-2649
Info: 606-929-5504
80 KOA Lane
Argillite, KY 41121Get Directions
Second Year : Come set up a table and sell those items from Spring Cleaning. Call our office for details
Bring along a sombrero and join us for Cinco del Mayo weekend. Crafts and activities all weekend.


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Slinger Vacation Packages
From Slinger, Milwaukee is 30 miles (48 kilometers) to the southeast, while Chicago is 110 miles (177 kilometers) to the south.
Slinger isn't the only place in this intriguing region that is worth experiencing; DIY adventurers should set out to see the charms of the surrounding scenery, towns and cities. Places in the vicinity of Slinger are Oconomowoc, 19 miles (31 kilometers) to the northeast, and Pewaukee Lake, 19 miles (31 kilometers) to the north.
Milwaukee, is another interesting place to discover. It is around 30 miles (48 kilometers) from Slinger and offers a wide range of visitor attractions, such as Milwaukee Art Museum, Milwaukee Public Museum and Mitchell Park Horticultural Conservatory. Milwaukee Public Library, Frontier Airlines Center and Wisconsin State Fair Park are just a sample of the other interesting sights here that should be included on every visitor's to-do list.
For a budget-friendly, outdoor experience that everyone will like, take the time to visit the region's open-air markets.
Cycling and shore fishing are just a few of the low stress activities in the Southeast Wisconsin area.
Even the best vacation can't last forever, so when it's time go home, head to the nearest major airport in Milwaukee, 35 miles (56 kilometers) to the southeast.
Accommodation in Slinger
There are currently no vacancies for Slinger listed on the Expedia travel page. Though, Hartford, some 5 miles (8 kilometers) away, features a good selection of hotels that can be easily booked. The nicest options in Hartford are the AmericInn Hotel & Suites Hartford and Super 8 - Hartford.


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Joanne Pyszczek wrote:
> I'd be curious to know what people consider there best increasers.
> New plants of mine that seem to increse the best were Pandora's Box,
> Cotillion and Kifukurin Ko Mame. I've been impressed with the late
> season growth on these. What are some others
> Joanne
> email@example.com
> ---------------------------------------------------------------------
> To sign-off this list, send email to firstname.lastname@example.com with the
> message text UNSUBSCRIBE HOSTA-OPENJoanne
I have found a general "good" increase in my Hostas this year. You are
right about Pandoras Box-- It has multplied very well
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Customer Reviews:
Comments: Great product. It's got great grip, looks much better than any tape job that I've ever done, and is durable. I'd definitely recommend it.
From: Matt, Ellington, CT
Comments: Tight fit, looks just like tape except doesn't fray or wear down, great grip.
From: Chris, IL
Contribute Your Feedback
Use this form or email email@example.com to contribute your experiences with this product. If you'd prefer, you can send an email to firstname.lastname@example.com. Although not necessary, it's interesting to have a first name, city, state and country (no email addresses will be used). Any information submitted becomes the property of Ice Warehouse.


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Cheryl Roberts
(888) 552-5228
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Cheryl Roberts
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Property DetailsOne of two available undeveloped adjacent lots in Palma Sola Park offers plenty of options. Just under a 1/2 acre, this lot is spacious for building and is outside required flood insurance areas. Combined with neighboring lot to the east PI# 3847200007 would offer the opportunity to build 2 homes side by side or a total .90 acre to for one home if you desire more space. Residents of Palma Sola Park have the opportunity to join the popular Palma Sola Yacht Club with boat slips based on availability. Located 2 blocks from scenic Palma Sola Bay Causeway, a popular spot for water sports & a dog friendly beach. Just 3 miles directly west on Manatee Ave. to the gulf beaches of Anna Maria Island.
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Additional Property InformationThis land/lot listing is located at 7617 Manatee Ave W, Bradenton, FL. The property is currently selling for $145,000.
This property is presented by Cheryl Roberts of Michael Saunders & Company based in Florida.


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Nokia Branded Phones, Tablets Returning to Market in Licensing Deal
The Nokia announcements won't likely move buyers much at this point today, said Olds. "I think consumers are overwhelmed with the choices in the phone/tablet/mobile market and won't really notice either the exit of a player or [the arrival of] a new player. I think this is too little, too late for the Nokia name and their partners. The world has changed since the time they were phone powers, and there really isn't a ready-made place for them in the market." Another analyst, Charles King, principal of Pund-IT, said he can imagine that some consumers in Europe could care about the latest Nokia developments, since the Nokia brand still carries weight in some quarters there. But whether the devices would be successful there depends on design, features and pricing, he added. "Suffice it to say that HMD Global has its work cut out for it." Overall, "it's difficult to imagine any scenarios where this deal will be successful," said King. "Given the ongoing challenges of Microsoft's efforts around smartphones, in general, and Nokia, in particular, these deals smack of a 'Hail Mary' pass thrown with an extra ladle of genuflection. At the same time, such efforts have produced winners; so while positive results seem unlikely, the effort itself may be worthwhile." The Microsoft and Nokia brands have been tied together for years, first because Nokia was a Microsoft premier Windows Phone partner for a long time, and later after Nokia sold its smartphone division to Microsoft in April 2014 for $7.1 billion. With that sale, Nokia left a business in which it had been a major player for more than a decade. It looked like Nokia was done with smartphones.The sale of the Nokia phone business to Microsoft included a clause that prevents Nokia from re-entering the smartphone market until the fourth quarter of 2016, which is why the latest phone licensing plans are arriving now. Since the Nokia acquisition, Microsoft's Lumia line of Windows-based phones, despite being generally well-received, has barely made a dent in unseating Android- and iOS-based smartphones from the top of the smartphone market. In April, Nokia announced the acquisition of digital health tracking company Withings S.A. for $192.6 million as it moves to position itself right into the mix of the connected health marketplace. The move is aimed at also giving Nokia more clout in the Internet of things marketplace as it works to tie its products into a tighter relationship with consumers. The acquisition was also made because health care is expected to be one of the largest vertical markets in the IoT, with analysts forecasting that mobile health will be the fastest-growing health care segment from 2015 to 2020, according to Nokia.
That changed in July 2015, when Nokia confirmed its plans to find a licensing partner that would build, distribute and sell Nokia-branded phones once again, using the company's vast design and engineering resources, according to an earlier eWEEK story. Once Nokia sold its manufacturing, marketing and channel distribution capabilities to Microsoft, it no longer had the infrastructure to again start building and selling phones on its own. Nokia had done something similar in November 2014, when the company announced that its first Android tablet computer, the N1, would be built under license by third-party device manufacturer Foxconn.
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2009 (Senior): Had started the season's first six contests at strong safety until a pectoral muscle injury sustained in Game 6 ended his season...Named in the season's opening week one of five permanent team captains, one of two on defense (also Nick Oppenneer)...With the schedule allowing his dad the time to make a rare appearance at a CSU game, had six tackles in resounding 23-17 win at Colorado (Sept. 6); also assisted in Elijah-Blu Smith's first-quarter interception by crushing the intended receiver, Scotty McKnight...In win vs. Weber State (Sept. 12), combined with Alex Williams to drop Walter Payton award candidate Treyvn Smith for a loss on a first-quarter carry...In assertive 35-20 win vs. Nevada (Sept. 19), his third-down blitz pressure on the passer led to a tackle for loss an eventual second-quarter punt; later, made a diving interception of an overthrown pass to set up a third-quarter touchdown after replay review confirmed the turnover...Led the team with 12 tackles in conference-opening loss at No. 19 BYU (Sept. 26), including a big stick to drop RB Bryan Kariya on a 1-yard screen pass; the dozen stops were his most in a game since he had 12 vs. TCU, 11/25/06...In bitter 31-29 loss at Idaho (Oct. 3), registered his first career sack, dropping Nathan Enderle on third down to force a punt, setting up a critical Rams TD drive...In 24-17 loss vs. Utah (Oct. 10), had seven stops, including a touchdown-saving tackle early in the fourth quarter with the Rams protecting a 17-10 lead; sustained the season-ending injury late in the game, on a goal-line play...Earned academic all-conference following the season.
2008 (Junior): Played in seven games with six starts as the Rams' free safety...Posted 28 tackles (21 solo) with one interception, one other pass broken up and a pair of forced fumbles...In first victory, vs. Sacramento State (Sept. 6), forced a first-quarter fumble...One year after his `07 season ended in his native Houston hours before the Rams were to meet the Cougars, sealed the Rams' 28-25 victory over Houston (Sept. 20) with a spectacular, leaping interception in the end zone with 2 seconds remaining; the athletic theft redeemed Kubiak for a tough-to-avoid facemask penalty that set up Houston inside the Rams' red zone with less a minute to play...In Cougars contest, in front of his family -- stripped of its power back home by Hurricane Ike -- tied for the team lead with nine tackles (seven solo), and blasted Houston's Patrick Edwards to break up a third-down pass, stopping a first-quarter Houston drive...At Cal (Sept. 27), forced a first-quarter fumble from Jahvid Best (recovered by teammate Wade Landers), ending a Bears drive in CSU territory, then dropped RB Shane Vereen on third down to force a third-quarter punt...Picked off an Omar Clayton pass in the Rams' end zone early vs. UNLV (Oct. 4), but a roughing-the-passer penalty nullified the play; rolled his ankle out of bounds on the ensuing return, and did not return...Missed ensuing four games, then returned to start at Air Force (Nov. 8), but incredibly sustained a knee injury on the game's first snap and did not return...Knee injury sidelined him for the final two regular-season games, but the Rams' bowl berth allowed him to return in a reserve role vs. Fresno State in the New Mexico Bowl (Dec. 20).
2007 (Junior): After a series of hard-luck circumstances, elected to petition the MWC for a medical hardship waiver and played in 2008 as a redshirt junior...Played in just two games, both starts, and totaled 12 tackles (three solo)...Was seemingly in on every play over the season's first two games...Collected eight stops (six solo) in season opener at Colorado (Sept. 1)...Had four tackles (three solo) vs. Cal. (Sept. 1)...Having anticipated a return to his native city for months, instead endured one of the most miserable weekends of his life at Houston (Sept. 22); felt ill during pregame warmups, then was sent to a hospital, where he spent two nights after being diagnosed with low blood pressure and internal bleeding due to an ulcer...Snapped his 14-game starting streak at Houston...Returned to Colorado Sept. 25, and missed the ensuing two contests before opting for the medical waiver prior the Oct. 13 Air Force game.
2006 (Sophomore): Started at safety in all 12 games...Led the team with 90 total stops and added six tackles for loss...Recorded five total tackles and added a fumble recovery at San Diego State (Dec. 2)...Led the team with 12 total tackles vs. TCU (Nov. 25)...Was second on the team with nine total tackles at Utah (Nov. 11), and registered his first career interception...Was second on the team with 10 total stops, including one for loss, vs. BYU (Nov. 4)...Had six total tackles at Wyoming (Oct. 21)...Posted a career-high 19 tackles to lead the team at Air Force (Oct. 12)...Was first on the team with seven total tackles, and added two pass break-ups, vs. UNLV (Oct. 7)...Was second on the team with nine tackles (two for loss), at Nevada (Sept. 16).
2005 (Freshman): Named to academic all-conference team...Also earned CSU's team award for academic excellence...Played in 11 of 12 games on special teams and in the secondary as a reserve...Was the first true freshman to see action for the Rams in 2005...Finished the season with nine tackles, six unassisted.
High School: Played both offense and defense at Regis High School in Denver, Colo. ...A three-year starter as a wide receiver and defensive back, made 102 total tackles (54 solo) and two interceptions as a senior...Caught 54 passes for 795 yards, fifth-most in the state's Class 5A division...Had 12 career interceptions at Regis...Named to all-Colorado team by the Rocky Mountain News...Also was a standout basketball and baseball player...Coach was former Denver Broncos linebacker Jim Ryan.
Personal: Given name Klint Alexander Kubiak...Born Feb. 17, 1987, in Houston, Texas...Is engaged to CSU volleyball student-athlete Tessa Nelson, the preseason Co-Player of the Year in the Mountain West Conference, with plans for a summer 2010 wedding...Son of Gary and Rhonda Kubiak...Has two brothers, including Klay, a sophomore QB on the CSU roster...Other brother, Klein, is a freshman WR at Rice...Father, Gary, is the head coach of the NFL's Houston Texans and went to five Super Bowls with the Denver Broncos, including three during his nine-year playing career...Majoring in restaurant and resort management...Hometown: Englewood, Colo.
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