text: OverviewBack to top NOW Foods - Boswellia Extract Standardized Extract Double Strength 600 mg. - 90 Vegetarian Capsules Item# :108234UPC# :733739046345 Brand:NOW Foods Size/Form:90 Vegetarian Capsules Ship Weight:0.95 Servings:90 Dosage:1 Capsule(s) Now Foods Double Strength Boswellia Extract Standardized Extract 600 mg. - 90 Vcaps Now Foods Double Strength Boswellia Extract Standardized Extract is standardized to contain min. 65% Boswellic Acids, the active constituents of the herb Boswellia serrata, also known as Frankincense. Now Foods Double Strength Boswellia Extract Standardized Extract's properties have been known to herbalists and prized since antiquity and recent scientific research has validated its putative uses. Studies indicate that Now Foods Double Strength Boswellia Extract Standardized Extract can help to support a healthy inflammatory response through their effects on the 5-LOX and COX-2 enzymes, as well as on other cellular signaling pathways. Double Strength Standardized Extract 600 mg Healthy Inflammatory Response Plus Turmeric Root A Dietary Supplement Vegetarian Formula NOW's MissionThe NOW mission is - To provide value in products and services that empower people to lead healthier lives. NOW Foods is an award-winning and highly respected manufacturer of vitamins, minerals, dietary supplements and natural foods. In 1948, with the natural food and supplement industry in its infancy, entrepreneur Paul Richard paid $900 for the purchase of Fearn Soya Foods—a Chicago based manufacturer of grain and legume-based products. This began a six-decade legacy of providing health-seeking consumers with high-quality, affordable nutrition products. History of NOWIn 1968, NOW Foods was founded under the belief that good health was not a luxury available only to the wealthy. 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NOW products are being tested to determine such things as effectiveness for joint support, quality of life for cancer patients, cardiovascular support, and athletic endurance. Effective products mean health and wellness benefits, which mean quality. GMP Quality AssuredNOW's GMP certification covers standard operating procedures, employee training, product specifications, expiration dating, vendor certifications and much more. Standard operating procedures include sampling and testing incoming materials according to NOW Foods specifications, inspecting manufacturing processes, and testing finished products to specifications. Tests include organoleptic evaluation (human senses such as sight, taste, smell), physical testing of tablets and capsules, chemical identity of ingredients, potency and contamination testing by the company's in-house state-of-the-art analytical testing lab, as well as microbiological testing by NOW Foods' in-house rapid analysis microbiological lab.


text: Hull FC and Wigan Warriors met at Wembley for Rugby Leagues big day out the Tetleys Challenge Cup Final 2013. It was a re-run of the 1985 Final that produced one of the finest games ever seen at the old Stadium. This time Tomkins and co were up against a Hull FC side that defeated Warrington Wolves in the semi-finals to move a step closer to claiming their first piece of silverware since 2005. Featuring the Final in full celebrations interviews and highlights from the key matches on the Road to Wembley this is the perfect souvenir of yet another memorable afternoon in rugby leagues most famous competition. Official Merchandise of The Rugby Football LeagueCheck stock and price

subset: commoncrawl


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Season 2 of the Overtime podcast is here! Listen to the first episode with Meg Robichaud now.
One of the things we really wanted to do at Medium is make our creators look great. In this concept we explored not only delivering a profile but more a portfolio that an author could be proud of.
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Take a pan and heat over medium high. Melt butter. Add the leeks and sugar. Saute till translucent for about 5 minutes. Add salt and pepper and sauté. Add lettuce, parsley and peas. Add 1 cup chicken stock. Cover and cook for approximately 5 minutes (till peas are nice and tender). Add remaining chicken stock and boil. Puree the soup well. Take another pot and pour the strained soup liquid. Serve.
Serving size Per servingComplete recipe
Calories 147 Calories from Fat 46
% Daily Value*
Total Fat 5 g8%
Saturated Fat 2.1 g10.5%
Trans Fat 0 g
Cholesterol 12.6 mg4.2%
Sodium 462.3 mg19.3%
Total Carbohydrates 16 g5.4%
Dietary Fiber 2.5 g9.9%
Sugars 6.6 g
Protein 9 g17.5%
Vitamin A 45.1%
Vitamin C 30%
Calcium 3.2%
Iron 9.6%
*Based on a 2000 Calorie diet


text:
Browse Batteries for Verizon iPhone 4
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|TYLT Energi+ Backpack w/ Built-in 10,400mAh Battery for Verizon iPhone 4
|The removable 10,400mAh rechargeable Lithium Ion battery provides up to 4 full smartphone charges or 1 full tablet charge. With over 1,450 cubic inches of internal space and 13 total pockets
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|Ghostek 7000mAh NRGbag Energy Water Resistant Backpack for Verizon iPhone 4
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text:
- Score:
- rated 4.08 / 5 stars
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- 140,588 Views
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- Comedy - Parody
- collab
- nintendo
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- Apr 12, 2011 | 8:52 PM EDT
- Frontpaged April 13, 2011
- Daily 4th Place April 13, 2011
If you liked this, check these out!
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Author Comments
Rated 3.5 / 5 stars
YAaaay kirby.
Almost as good as the original Kirby collab@!
Almost as good? Are you nuts? It's way better!
Rated 5 / 5 stars
Kirby = \/\/1l\l
This was totally awesome. Had me laughing the whole way. I was just thinking there should be a new kirby game cuz i havent seen a classic kirby in a while and BOOM this showed up. Man this was funny. Especially the last part due to my obsession with ninjas. Enemy Kill.exe not found? WTF?
Rated 5 / 5 stars
LMAO ONE OF THE FUNNiest FLASHES IVE EEVVAAR SEEN LMAO i LOLED on almost all of em, especially the salesman one with the pot LLLOOOLLL
Rated 4.5 / 5 stars
Very fun
The Last one was the best. Kirby got Kick-ass!!
Rated 3.5 / 5 stars
I agree with Andropinus on the mix of qualities thing. But I'm too lazy to go through all of them, so I'll just give you a 7 overall, because I loved the use of :3 in the last one. "Okay go :3" made me smile.


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Click on the "Convert & Download Video + MP3" button to start the video conversion. After the video conversion is finished, you can download the converted video in the formats MP4 or 3GP (for mobile phones) or you can download the converted audio as a MP3 music file. This online service is completely for free.
Stone Sour's video for 'Hesitate' from the album, Audio Secrecy - available now on Roadrunner Records. Download now on iTunes: http://smarturl.it/audiosecrec...
DMCA: Are you the owner of this convertable material and do you want to disable the conversion of your media on clip.dj? Then you can request a conversion block for this video.203
Stone Sour performs "Hesitate" acoustic for WAAF listeners at Wachusett Mountain.
I do not own the rights to this song.All rights reserved to the owner(s).
Stone Sour - Hesitate - Audio Secrecy - 2010 - Hard Rock.
Stone Sour in the studio record 'Hesitate' off the album Audio Secrecy.
From their 2010 album "Audio Secrecy".
Stone Sour- Imperfect.
it's CMFT.
Photograph by Adolfo Valente.
hesitate by stone sour with lyrics on screen correct any wrong lyrics please :) You were my fire, so I burned... til' there was nothing ...
Corey Taylor (Stone Sour) - Imperfect (Legendado)


text:
Bosentan reverses the pro-fibrotic phenotype of systemic sclerosis dermal fibroblasts via increasing DNA binding ability of transcription factor Fli1
DOI: 10.1186/ar4529
© Akamata et al.; licensee BioMed Central Ltd. 2014
Received: 26 May 2013
Accepted: 21 March 2014
Published: 3 April 2014
Although the pathogenesis of systemic sclerosis (SSc) still remains unknown, recent studies have demonstrated that endothelins are deeply involved in the developmental process of fibrosis and vasculopathy associated with SSc, and a dual endothelin receptor antagonist, bosentan, has a potential to serve as a disease modifying drug for this disorder. Importantly, endothelin-1 (ET-1) exerts a pro-fibrotic effect on normal dermal fibroblasts and bosentan reverses the pro-fibrotic phenotype of SSc dermal fibroblasts. The purpose of this study was to clarify the details of molecular mechanisms underlying the effects of ET-1 and bosentan on dermal fibroblasts, which have not been well studied.
The mRNA levels of target genes and the expression and phosphorylation levels of target proteins were determined by reverse transcription real-time PCR and immunoblotting, respectively. Promoter assays were performed using a sequential deletion of human α2 (I) collagen (COL1A2) promoter. DNA affinity precipitation and chromatin immunoprecipitation were employed to evaluate the DNA binding ability of Fli1. Fli1 protein levels in murine skin were evaluated by immunostaining.
In normal fibroblasts, ET-1 activated c-Abl and protein kinase C (PKC)-δ and induced Fli1 phosphorylation at threonine 312, leading to the decreased DNA binding of Fli1, a potent repressor of the COL1A2 gene, and the increase in type I collagen expression. On the other hand, bosentan reduced the expression of c-Abl and PKC-δ, the nuclear localization of PKC-δ, and Fli1 phosphorylation, resulting in the increased DNA binding of Fli1 and the suppression of type I collagen expression in SSc fibroblasts. In bleomycin-treated mice, bosentan prevented dermal fibrosis and increased Fli1 expression in lesional dermal fibroblasts.
ET-1 exerts a potent pro-fibrotic effect on normal fibroblasts by activating "c-Abl - PKC-δ - Fli1" pathway. Bosentan reverses the pro-fibrotic phenotype of SSc fibroblasts and prevents the development of dermal fibrosis in bleomycin-treated mice by blocking this signaling pathway. Although the efficacy of bosentan for dermal and pulmonary fibrosis is limited in SSc, the present observation definitely provides us with a useful clue to further explore the potential of the upcoming new dual endothelin receptor antagonists as disease modifying drugs for SSc.
Systemic sclerosis (SSc) is a multisystem connective tissue disease characterized by immune abnormalities, vascular injuries, and fibrosis of skin and certain internal organs [1]. Although the pathogenesis of SSc still remains unclear, it has been believed that fibroblast activation is a final consequence following inflammation, autoimmune attacks, and vascular damage. A wealth of evidence suggests that, once activated, SSc dermal fibroblasts establish a self-activation system by autocrine transforming growth factor (TGF)-β stimulation at least partially via upregulating cell surface receptors for latent-form TGF-β, such as integrin αVβ3, integrin αVβ5, and thrombospondin-1 [2–6]. A plausible strategy to treat fibrosis in SSc is to block the autocrine TGF-β signaling in SSc fibroblasts and better understanding of its molecular mechanism is necessary to develop the treatment for this complicated disorder [1, 7].
The endothelins are a family of 21-amino-acid peptides mainly produced by endothelial cells, which consist of three isoforms, including endothelin-1 (ET-1) and the related peptides endothelin-2 and -3. In addition to a potent vasoconstrictive effect, ET-1 possesses a wide range of biological effects on different cell types. Several lines of evidence have demonstrated that ET-1 plays a pivotal role in the process of fibroblast activation as a downstream target of TGF-β [8–11]. TGF-β1 induces the expression of ET-1 through Smad- and activator protein-1/c-Jun N-terminal kinase-dependent signaling in human dermal fibroblasts, while through a Smad-independent ALK5/activator protein-1/c-Jun N-terminal kinase-dependent signaling in human lung fibroblasts, and the ability of TGF-β1 to trigger the pro-fibrotic gene program is dependent on ET-1 in both of these cells [9, 12]. In animal models, forced expression of ET-1 accelerates dermal wound healing as well as TGF-β1, while blockade of endothelin signaling with bosentan, a dual endothelin receptor antagonist, significantly inhibits the effect of TGF-β1 on dermal wound healing [12]. Importantly, bosentan also attenuates bleomycin (BLM)-induced skin fibrosis in animal models [12]. Thus, endothelins are potentially implicated in the pathogenesis of fibrotic disorders.
The role of ET-1 has also been well-studied in SSc. Circulating ET-1 levels are elevated in diffuse cutaneous SSc and limited cutaneous SSc patients compared with healthy controls, and in limited cutaneous SSc patients with pulmonary arterial hypertension as compared to those without [13, 14], suggesting the involvement of ET-1 in the development of fibrotic and vascular involvement associated with SSc. Clinically, bosentan has been shown to prevent the development of new digital ulcers in SSc patients by two high-quality randomized clinical trials [15, 16]. As for pulmonary arterial hypertension associated with SSc, bosentan mostly prevents the exacerbation of hemodynamic parameters and improves exercise tolerance evaluated by 6-minute walk distance [17–19]. These clinical data suggest that bosentan is useful for the treatment of vascular involvement in SSc. In contrast, the clinical efficacy of bosentan for interstitial lung disease (ILD) is relatively limited [20, 21], though bosentan reverses the pro-fibrotic phenotype of cultured SSc lung fibroblasts [8–11]. Since this discrepancy between experimental data and clinical observation may be partly due to the relatively lower tissue distribution of bosentan (approximately 1%) [22], endothelin receptor antagonists with better tissue distribution have a potential to improve the pathological fibrosis in SSc.
As described above, although ET-1 is a potent pro-fibrotic peptide and bosentan exerts a novel anti-fibrotic effect, the detailed molecular mechanism explaining these observations has still remained unknown. Therefore, we herein investigated the mechanisms by which ET-1 drives a pro-fibrotic gene program in normal dermal fibroblasts and bosentan reverses the pro-fibrotic phenotype of SSc dermal fibroblasts and the experimental dermal fibrosis in animal models.
Anti-Fli1 antibody for immunoblotting and immunofluorescence and anti-protein kinase C (PKC)-δ antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-Fli1 antibody for immunohistochemistry was obtained from BD Biosciences (San Diego, CA, USA). Antibodies against c-Abl and phospho-c-Abl (Tyr245) were bought from Cell Signaling (Danvers, MA, USA). Antibodies for β-actin and α-smooth muscle actin (α-SMA) and antibody for type I collagen were products from Sigma-Aldrich (St. Louis, MO) and Southern Biotech (Birmingham, AL, USA), respectively. The polyclonal rabbit anti-phospho-Fli1 (Thr312)-specific antibody was generated as described previously [23]. Bosentan was a gift from Actelion Pharmaceuticals (Allschwil, Switzerland). Smad3 inhibitor SIS3 was purchased from Calbiochem (San Diego, CA, USA).
Cell cultures
Human dermal fibroblasts were obtained by skin biopsy from the affected areas (dorsal forearm) of eight patients with diffuse cutaneous SSc with less than 2 years of skin thickening and from the corresponding area of eight closely matched healthy donors. Fibroblasts were cultured in Dulbecco's modified eagle medium with 10% fetal calf serum, 2 mM L-glutamine, and the antibiotic antimycotic solution. These cells were individually maintained as monolayers at 37°C in 95% air, 5% CO2. All studies used cells from passage number three to six. The study was performed according to the Declaration of Helsinki and approved by the ethical committee of the University of Tokyo Graduate School of Medicine. Written informed consent was obtained from all of the patients and healthy controls.
Cell viability assay
Cell viability was evaluated by the trypan blue exclusion test. Cells were treated with the indicated concentration of bosentan. Cell viability was examined at 24 and 48 hours. Stained (dead) and unstained (viable) cells were counted with a hematocytometer.
Confluent quiescent fibroblasts were serum-starved for 48 hours and harvested. In some experiments, cells were stimulated with ET-1 or bosentan for the indicated period of time before being harvested. Whole cell lysates and nuclear extracts were prepared as described previously [2, 24]. Samples were subjected to sodium dodecyl sulfate-polyacrylamide gels electrophoresis and immunoblotting with the indicated primary antibodies. Bands were detected using enhanced chemiluminescent techniques (Thermo Scientific, Rockford, IL, USA). According to a series of pilot experiments, anti-Fli1 antibody and anti-phospho-Fli1 (Thr312)-specific antibody work much better in immunoblotting using nuclear extracts and whole cell lysates, respectively.
RNA isolation and reverse transcription (RT) real-time PCR
Total RNA was isolated from normal and SSc fibroblasts with RNeasy spin columns (Qiagen, Crawley, UK). One μg of total RNA from each sample was reverse-transcribed into cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). Real-time quantitative PCR was performed using Fast SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA) on ABI prism 7000 (Applied Biosystems) in triplicates. Human α2 (I) collagen (COL1A2) and Fli1 mRNA levels were normalized to human 18S rRNA mRNA levels. The sequences of primers for COL1A2, Fli1, and 18S rRNA were obtained from previous publications [25, 26]. The ΔΔCt method was used to compare target gene and housekeeping gene (18S rRNA) mRNA expression.
Plasmid construction
Generation of a series of 5′-deletions of COL1A2/chloramphenicol acetyltransferase (CAT) construct consisting of the COL1A2 gene fragments (+58 to −353, −264, −186, or −108 bp relative to the transcription start site) linked to the CAT reporter gene was done as previously described [27, 28].
The evaluation of COL1A2 promoter activity by RT real-time PCR
Normal or SSc fibroblasts were grown to 50% confluence in 100-mm dishes, transfected with the indicated constructs along with pSV-β-galactosidase (β-GAL) using FuGENE6 (Roche Diagnostics, Indianapolis, IN, USA). After overnight incubation at 37°C, some cells were further stimulated with ET-1 or bosentan for 24 hours. The cells were harvested and CAT and β-GAL mRNA levels were determined using RT real-time PCR. Transfection efficiency was normalized by β-GAL mRNA levels. In some samples, it was confirmed that this method reproduces the results of relative promoter activity evaluated by the canonical method of CAT reporter assay using [14C]-chloramphenicol. The sequences of primers were as follows: CAT forward 5′-TTCGTCTCAGCCAATCCCTGGGTGA-3′ and reverse 5′-CCCATCGTGAAAACGGGGGCGAA-3′; β-GAL forward 5′-TCCACCTTCCCTGCGTTA-3′ and reverse 5′-AGAAGTCGGGAGGTTGCTG-3′.
DNA affinity precipitation assay
DNA affinity precipitation assay was performed as described previously [29]. Briefly, nuclear extracts prepared from dermal fibroblasts were incubated for 10 minutes at 4°C with gel shift binding buffer, and 20 μg of poly (dI-dC) in a final volume of 1 ml. Pre-clearing was performed by adding streptavidin-coupled agarose beads and incubating the mixture for 30 minutes at 4°C. After centrifugation, the supernatant was incubated with 500 pM of COL1A2-EBS oligonucleotide, which corresponds to bp −307 to −269 of COL1A2 promoter, or COL1A2-EBS-M oligonucleotide, which has a mutated Ets binding site (EBS) of COL1A2-EBS oligonucleotide, overnight at 4°C. Then, streptavidin-coated agarose beads were added, followed by a further 2-hour incubation at 4°C. The protein-DNA-streptavidin-agarose complex was washed twice with Tris-ethylenediaminetetraacetic acid (EDTA) including 100 mM NaCl, twice with gel shift binding buffer, and once with PBS. Precipitates were subjected to immunoblotting with anti-Fli1 antibody.
Chromatin immunoprecipitation (ChIP) assay
ChIP assay was carried out using EpiQuik ChIP kit (Epigentek, Farmingdale, NY, USA). Briefly, cells were treated with 1% formaldehyde for 10 minutes. The cross-linked chromatin was then prepared and sonicated to an average size of 300 to 500 bp. The DNA fragments were immunoprecipitated with anti-Fli1 antibody at 4°C. As a negative control, normal rabbit IgG was used. After reversal of crosslinking, the immunoprecipitated chromatin was quantified by RT real-time PCR. The primers were as follows: COL1A2/F-404, 5′-CTGGACAGCTCCTGCTTTGAT-3′; COL1A2/R-233, 5′-CTTTCAAGGGGAAACTCTGACTC-3′.
BLM-induced murine model of SSc
BLM (Nippon Kayaku, Tokyo, Japan) or PBS was injected subcutaneously into the back of C57BL/6 mice daily for 3 weeks, as described previously [30].
Immunohistochemistry with Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) was performed on formalin-fixed, paraffin-embedded tissue sections using anti-Fli1 antibody according to the manufacturer's instruction.
Rabbit anti-Fli1 antibody and mouse anti-α-SMA antibody were used as primary antibodies and Alexa Fluor 546 goat anti-rabbit IgG antibody (Invitrogen, Carlsbad, CA, USA) and fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse IgG antibody (Roche) were used as secondary antibodies. Coverslips were mounted by using Vectashield with DAPI (Vector Laboratories), and staining was examined by using Bio Zero BZ-8000 (Keyence, Osaka, Japan) at 495 nm (green), 565 nm (red), and 400 nm (blue).
Statistical analysis
Statistical analysis was carried out with the Mann-Whitney U-test to compare the distributions of two unmatched groups. The paired t-test was used for the comparison of paired data after confirming the normal distribution. Statistical significance was defined as a P-value of <0.05.
ET-1 rapidly induced the expression of type I collagen via the non-Smad signaling pathway
We next investigated the time course of ET-1-dependent induction of type I collagen in normal dermal fibroblasts. As shown in Figure 1C, the increase in the mRNA levels of the COL1A2 gene was observed modestly but significantly as early as 15 minutes and reached a maximum around 30 to 60 minutes after the ET-1 stimulation. This observation was also reproduced at protein levels by immunoblotting (Figure 1D). As Smad3 has a big impact on the TGF-β-dependent regulation of type I collagen expression, we also investigated the effect of Smad3 inhibitor SIS3 on the ET-1-induced mRNA expression of the COL1A2 gene. As shown in Figure 1E, SIS3 did not affect the mRNA levels of the COL1A2 gene induced by ET-1 stimulation. Collectively, these results indicate that ET-1 rapidly increases the expression of type I collagen via the non-Smad signaling pathway.
The responsive element of COL1A2 promoter to ET-1 was located between −353 and −264 bp, which includes the Fli1 binding site
ET-1 induced the phosphorylation of Fli1 at threonine 312 and decreased its binding to the COL1A2 promoter
ET-1 promoted the nuclear localization of PKC-δ by activating c-Abl
Bosentan, a dual endothelin receptor antagonist, decreased the expression of the COL1A2 gene by reversing the transcriptional activity of Fli1 in SSc dermal fibroblasts
Previous reports demonstrated that bosentan, a dual ET receptor antagonist, reverses a pro-fibrotic phenotype of SSc fibroblasts [8]. However, the detailed mechanism by which bosentan exerts its prominent anti-fibrotic effect on SSc fibroblasts has still remained unknown. We previously demonstrated that Fli1 deficiency contributes to the establishment of the pro-fibrotic phenotype in SSc fibroblasts and imatinib mesylate, which targets the c-Abl/PKC-δ/Fli1 pathway, reverses the pro-fibrotic phenotype of these cells [33, 36]. Given that SSc fibroblasts are constitutively activated by autocrine stimulation of transforming growth factor- β (TGF-β), a potent inducer of ET-1, and produces an excessive amount of ET-1 [4, 9, 31, 37, 38], autocrine ET-1 appears to be involved in the self-activation system in SSc fibroblasts. The present observation that ET-1 inactivates the transcriptional activity of Fli1 suggests that the blockade of autocrine ET-1 by bosentan reverses the pro-fibrotic phenotype of SSc fibroblasts by reactivating the transcriptional repressor activity of Fli1. To address this issue, we performed a series of experiments using cultured SSc dermal fibroblasts.
Viability of systemic sclerosis dermal fibroblasts in the presence or absence of bosentan, which was evaluated by trypan blue exclusion test
Duration of treatment (h)
Bosentan (μM)
96.5 ± 0.05
98.8 ± 0.24
96.3 ± 0.70
97.3 ± 1.52
97.3 ± 1.13
97.8 ± 1.13
97.6 ± 1.68
96.3 ± 0.72
95.6 ± 0.64
96.2 ± 0.55
96.8 ± 0.25
95.1 ± 0.18
Bosentan increased the expression of Fli1 protein in lesional dermal fibroblasts of the BLM-induced murine model of SSc
This study was undertaken to clarify the molecular mechanism underlying the pro-fibrotic effect of ET-1 on normal dermal fibroblasts and anti-fibrotic effect of bosentan on SSc dermal fibroblasts. A series of experiments demonstrated that ET-1 activates the c-Abl/PKC-δ/Fli1 pathway and reduces the DNA binding ability of Fli1, resulting in the induction of type I collagen expression. Given that a pro-fibrotic phenotype of SSc fibroblasts is largely due to stimulation by autocrine TGF-β, a potent inducer of ET-1, and those cells produce a much larger amount of ET-1 than normal dermal fibroblasts [1, 7, 38], it was speculated that the blockade of ET-1-dependent signaling by bosentan reverses the pro-fibrotic phenotype of SSc dermal fibroblasts by increasing the DNA binding of Fli1 through the inhibition of the c-Abl /PKC-δ/Fli1 pathway, which is constitutively activated in those cells [33]. Supporting this hypothesis, exogenous ET-1 did not affect type I collagen expression, whereas bosentan suppressed the expression of c-Abl and PKC-δ, decreased the nuclear localization of PKC-δ and Fli1 phosphorylation at threonine 312, and eventually increased the DNA binding ability of Fli1 to the COL1A2 promoter resulting in the reduction of type I collagen expression in SSc dermal fibroblasts. Furthermore, bosentan increased the expression levels of Fli1 protein in lesional dermal fibroblasts of BLM-treated mice. As the protein stability of Fli1 increases when Fli1 binds to DNA, and is protected from degradation, these in vivo data suggest that bosentan prevents the development of dermal fibrosis in BLM-treated mice by increasing the DNA binding of Fli1 in lesional dermal fibroblasts. Collectively, these results indicate that the anti-fibrotic effect of bosentan on SSc dermal fibroblasts and BLM-induced SSc murine model is at least partly attributable to the increase in the DNA binding ability of Fli1.
ET-1 induces a pro-fibrotic phenotype in fibroblasts through increasing the expression of extracellular matrix (ECM) proteins, such as type I and III collagen and fibronectin, and decreasing the expression of matrix metalloproteinase 1 [39, 40], therefore the blockade of ET-1 signaling has been thought to be a potential therapeutic strategy for fibrotic disorders, including SSc. The first study of the anti-fibrotic effect of bosentan on SSc fibroblasts was reported in 2004 by Shi-Wen et al. A series of studies from their group demonstrated that bosentan suppresses the expression of α-SMA, type I collagen, fibronectin, and CCN2 in SSc lung fibroblasts and ET-1 is a downstream mediator of pro-fibrotic responses to TGF-β in human lung fibroblasts [8, 10, 11]. Following these, Lagares et al. [12] revealed that these previous findings in lung fibroblasts are reproducible in dermal fibroblasts in in vitro experiments and in vivo animal models. Thus, ET-1 and its receptor antagonists have currently drawn much attention in the field of research on the mechanism and treatment of fibrotic disorders, but the detailed molecular mechanism explaining their effects has remained to be clarified. Since ET-1 induces a prominent fibrotic response even in the absence of Smad2/3 activation, which are important intracellular second messengers of TGF-β signaling especially in the regulation of the fibrotic gene program, ET-1 appears to inactivate a potent master repressor acting on a set of fibrosis-related genes. Taking into account that gene silencing of Fli1 results in the induction of COL1A2 gene expression up to the level much greater than that achieved by TGF-β1 stimulation [23, 25, 29, 32, 33, 41], we speculated that Fli1 may be inactivated in response to ET-1 stimulation. Consistent with this idea, a responsive element of COL1A2 promoter to ET-1 was located between −353 and −264 bp, including the Fli1 binding site, and ET-1 activated c-Abl and PKC-δ, resulting in Fli1 inactivation through its phosphorylation at threonine 312. Although other mechanisms may be involved in the pro-fibrotic effect of ET-1, the current data indicate that Fli1 largely contributes to the mechanism explaining the potent pro-fibrotic effect of ET-1 in dermal fibroblasts.
Although bosentan attenuates BLM-induced dermal and pulmonary fibrosis in animal models [12, 42–44], its efficacy on skin sclerosis and ILD in SSc, to our best knowledge, has been reported to be limited [20, 21]. In addition to the difference in the pathological process between SSc and animal models, lower potential of bosentan for good tissue distribution, which is estimated around 1% [22], has been cited as a cause of the unsuccessful outcome of oral bosentan therapy against skin sclerosis and ILD in SSc. Considering that the average peak plasma concentration after a single dose of 125 mg of bosentan, which is a maximal dose clinically used in humans, is 1,584 ng/ml (2.87 μM) in Caucasian and 1,922 ng/ml (3.48 μM) in Japanese subjects [45], 10 μM of bosentan, which has been used for in vitro experiments with fibroblasts, appears to be much higher than the concentration of bosentan in human skin tissue. In the present study, bosentan suppressed type I collagen expression in a dose-dependent manner up to 40 μM without affecting cell viability in SSc dermal fibroblasts, suggesting that endothelin receptor antagonists with better tissue distribution and tolerability may have potential as disease-modifying drugs targeting skin sclerosis and ILD in SSc by directly inactivating fibroblasts.
According to a previous report by Wang et al. [46], the expression of Fli1 is strongly suppressed at the transcription level by an epigenetic mechanism in SSc dermal fibroblasts. The authors demonstrated that the acetylation levels of histone H3 and H4 are decreased, while the methylation levels of CpG islands are increased, in the promoter region of the FLI1 gene in SSc dermal fibroblasts compared with normal dermal fibroblasts, suggesting that the decrease in FLI1 gene expression contributes to the developmental process of SSc as a potential predisposing factor. The most important observation in the present study was that bosentan reversed the decreased expression of Fli1 protein without affecting its gene expression status in SSc dermal fibroblasts. These results suggest that bosentan can increase Fli1 protein levels regardless of the degree of epigenetic transcriptional suppression in those cells. Given that the reversion of epigenetically repressed disease-related genes by drugs is a general idea for the treatment of various diseases, bosentan may serve as a disease-modifying drug for SSc. Although the efficacy of bosentan on skin sclerosis and ILD is limited, recent papers demonstrated the potential of bosentan as a disease-modifying drug for SSc vasculopathy. In addition to the prevention of new digital ulcers by bosentan [15, 16], Guiducci et al. [47] revealed that one-year treatment with bosentan significantly decreases the number of advanced nailfold capillary changes, such as capillary disorganization, ramified capillaries, and capillary loss, while increasing the number of relatively early changes, including enlarged capillaries, megacapillaries, and hemorrhage, in SSc patients. These clinical findings suggest that the effect of endothelin receptor antagonists on SSc vasculopathy goes beyond the reversal of the potent vasoconstrictive effects of endothelin. This notion has been supported by some experimental data. For example, ET-1 has a potent mitogenic action on fibroblasts and vascular smooth muscle cells [48, 49]. Furthermore, ET-1 prolongs the survival of myofibroblasts by preventing apoptosis [8]. Moreover, ET-1 triggers the pathological inflammation by modulating the expression of cell adhesion molecules on endothelial cells [50] and by promoting the production of free radical and fibrosis-inducing cytokines, such as monocyte chemoattractant protein-1 and TGF-β, from macrophages [51]. These proliferative, pro-fibrotic, and pro-inflammatory effects of ET-1 contribute to the development of SSc vasculopathy. Considering that endothelial Fli1 deficiency contributes to the development of SSc vasculopathy [52], bosentan may exert its disease-modifying effect on SSc vasculopathy by increasing endothelial Fli1 expression. The research regarding the effect of bosentan on endothelial Fli1 deficiency is currently underway in our laboratory.
Similar to bosentan, our latest paper demonstrated that imatinib reverses the decreased expression of Fli1 in SSc dermal fibroblasts by increasing its protein stability and without affecting its gene expression status [33], suggesting that imatinib is a potential disease-modifying drug for SSc patients. Consistent with this idea, imatinib moderately improved skin sclerosis of SSc patients in a couple of case reports [53, 54] and case series [55–57]. Although imatinib was poorly tolerated and failed to show a significant effect on skin sclerosis of SSc patients in a couple of clinical trials [58], previous case reports suggest that some derivatives of imatinib with a better tolerability may be effective for skin sclerosis in a certain subset of SSc patients. Similarly, the clinical response of digital ulcers associated with SSc to bosentan varies from patients to patients [15, 16]. Given that SSc is a multifactorial disease caused by a complex interaction between hereditary factors and environmental influences, these clinical data suggest that imatinib and bosentan may target some disease-associated factors, leading to the modification of the natural disease course in a certain subset of SSc patients. Given that both of c-Abl tyrosine kinase inhibitors and endothelin receptor antagonists target Fli1, a potential predisposing factor of SSc, further studies on the association between the degree of Fli1 suppression and the clinical efficacy of these drugs may provide us some clue to properly select good responders and effectively administer these treatments for SSc patients.
In the present study, 200 nM of ET-1 showed the maximal stimulatory effect on type I collagen expression in normal human dermal fibroblasts. However, previous reports demonstrated that 100 nM of ET-1 exhibits a significant stimulatory effect on the expression levels of type I collagen, fibronectin, fibrillin-1, and α-SMA and the gel contraction in murine embryonic fibroblasts and human normal lung and dermal fibroblasts [8, 9, 12, 40, 59]. This discrepancy may be attributable to the different sensitivity of fibroblasts to ET-1 and/or to the different experimental conditions between each study, but the actual reason is unclear.
We herein reported the first study regarding the detailed molecular mechanism underlying the pro-fibrotic effect of ET-1 on normal dermal fibroblasts and the anti-fibrotic effect of bosentan on SSc dermal fibroblasts, in which transcription factor Fli1, a potential predisposing factor in SSc, is an important target. Although the efficacy of bosentan for dermal and pulmonary fibrotic conditions associated with SSc is limited, the present observation definitely provides us with a useful clue to further explore the potential of the upcoming new dual endothelin receptor antagonists with better clinical effects as disease-modifying drugs for SSc.
- β-GAL:
- bp:
base pairs
chloramphenicol acetyltransferase
- ChIP:
Chromatin immunoprecipitation
human α2 (I) collagen
Ets binding site
interstitial lung disease
phosphate buffered saline
protein kinase C
reverse transcription
- SSc:
systemic sclerosis
transforming growth factor.
We thank Tamami Kaga and Yoshiko Ito for the technical help in immunohistochemistry. This study was supported in part by grants from the Ministry of Health, Labor, and Welfare of Japan, from Japan Intractable Diseases Research Foundation, from Rohto Dermatology Prize, from JSID's Fellowship Shiseido Award, from the Mochida Memorial Foundation for Medical and Pharmaceutical Research, and from Japan Foundation for Applied Enzymology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.


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CroParadise True Green Apartment
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Description from the manager
Bedrooms: 3/
Bathrooms: 2/
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Type: Apt. / Condo
Book this 3 bedroom, 2 bathroom apt. / condo in Split today! A total of 8 people can sleep here comfortably.
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Tropical Fish Keeping - Aquarium fish care and resources (http://www.tropicalfishkeeping.com/forum.php)
- Beginner Saltwater Aquariums (http://www.tropicalfishkeeping.com/beginner-saltwater-aquariums/)
- - Nasty Spot Algae!! (http://www.tropicalfishkeeping.com/beginner-saltwater-aquariums/nasty-spot-algae-33662/)
Nasty Spot Algae!!
Just wondering, what is the best way to clean spot algae of your tank and to keep them from coming back? I have a 40 gl tall tank with about 30 gallon of live rock and 1 inch sand bed. My water paremters are where its suppose to be i get it tested everyweek. Tanks been up for about 2 months but technically the water and live rock and sand came from an existing established tank. I have 2 power compacts 30'' one 10000k and one actinic. Not worried about the sides and back wall but i'd like a clear view of the front. The sides and backwall is actually getting covered with coraline which i heard is a good sign but the front is also getting coraline which i'd also like to remove. So guys whats the trick to cleaning this spot algae off?
oh also all i have is one sifter some hermits and snails and some easy corals like pulsing xenis and zoas
Great question. I use a magnetic algae scrapper to remove brown algae from the front glass. Coraline algae is best removed with a razor blade.
yeah the brown algae comes off no problem with the magnetic scraper but doesnt even touch the spot algae
spot algae is annoying me so hard to clean
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Browse our new Atlas Portable Toilets site for detailed information on our portable toilets, locally manufactured and imported.
Tough portable toilets made from the highest quality LLDPE, guaranteed to deliver 10 years of dependable service.
Browse our new Atlas PPR Pipe site for detailed information on our corrosion resistant polypropylene piping system.
The first and only locally manufactured SABS SANS 15874 approved hot and cold water PPR pipe system in South Africa.
Browse our new Viking Sanitary Ware site for detailed information on our theft and vandal resistant sanware.
Purpose built to help you prevent abuse, and cut back on maintenance and replacement costs in the long run.
With our focus on innovation, we have added two new machines to our factory during 2013, incorporating the latest technology, improving quality and productivity.
We use temperature monitoring equipment and 3D technology to ensure that our products are manufactured within specification, ensuring absolute quality, and we are constantly improving our standards for the benefit of our customers.
Our research and development team is an integral part of our business, designing many great products, some of which are design protected and patented.
Our impressive range of products, which are supplied to a variety of industries and markets span from mobile huts, sanitation, tanks, drums, mining, bins, piping and accessories to general hardware and products.
We have an amazing and passionate team of professionals who are always looking forward to providing you with excellent products and services.
We believe that building strong relationships with our customers is what has enabled us to produce quality products that satisfy our customers' needs. We look forward to continuing this trend with you.


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Men's soccer
The NCAA Tournament bracket does not include Akron.
The Zips' season ends with a 13-5-1 record. Sinisa Ubiparipovic had a great season with seven goals and seven assists. Other than his MAC Player of the Year award, the season is a giant disappointment.
You really can't argue otherwise. The Zips were ranked in the preseason top 10, yet could not win the conference tournament or earn a berth in the 48-team NCAA Tournament.


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Samuelsen Evans posted an update 1 year, 1 month ago
Perfect Biotics – How Probiotics And Prebiotics Can Improve Your Life 6628
Perfect Biotics – Advice You Need To Know About Nutritional Probiotics 7206perfect bioticsprobiotics and probiotics help to keep the body working well daily. Though carbs fuel the body, probiotics and probiotics nourish it and makes it operate correctly. Therefore, it is very important be informed about the role of nutrition. Please read on to understand the best way to keep balanced and healthy diet.probiotics will help you find more from your workouts. This will help to stop soreness to help you return to hitting the gym faster.Your whole body must synthesize probiotics and probiotics. Knowing those are best together will yield the best results. A good example is how iron has difficulty being absorbed with calcium present. It's smart to drink some orange juice with the iron supplement while avoiding antacids and dairy food for at half an hour after taking iron.Calcium is integral for the making of your bones. Your system needs probiotic D, though, to correctly absorb the calcium. Sunlight, particular foods, and probiotic D supplements can deal with absorption. Many of these things enable your body to adopt in additional calcium.Milk and sun exposure are wonderful probiotic D sources. In the event you lack exposure to the dun, or avoid drinking milk, then it is vital that you have a probiotic D supplement. probiotic D plays a major role in relation to keeping your bones strong.You can get probiotic D from milk and exposure to the sun. When you are not really a big milk drinker or will not spend time and effort in the sun, you ought to take a supplement with probiotic D. This will help to fortify your bones.Vit A is extremely important it increase the immune system, betters vision and lowers cardiovascular disease risk. Will not exceed the RDA of 2300 IU because vast amounts could be toxic. Squash, carrots and dark leafy greens provide vit a.If you're postmenopausal, don't consume prenatal probiotics. A lot of women choose to take the probiotics with regard to their hair or nails. For post-menopausal women, these probiotics contain an excessive amount of iron.Supplements are serious business. Your body needs probiotics and prebiotics, nevertheless, you can take more than you need. This can happen in case you are taking plenty of supplements, and also the results can be dangerous. probiotic overdose symptoms vary with with all the probiotic, although the effects are generally unpleasant, and they also may even be fatal.The starting place in every supplement journey has your doctor test you for specific nutrient deficiencies. This is an issue that should offer a basis for your supplements, so you must know those you will need at the earliest opportunity.Exercise caution when using supplements. Never take over the recommended amount. This can happen if you are taking plenty of supplements, along with the results may be dangerous. Be sure that you speak with your doctor about the right doses. probiotic america You must eat more fresh produce and less canned. Though you need to eat a healthy diet plan, you must also consider taking a quality multi probiotic or probiotic supplements to improve your diet program.Try getting good manganese. This can help bones grow and heal. It can also help wounds heal. It will help you metabolize protein, cholesterol, and carbs. Almonds, black and green tea extract, beans, and cereals all have it. You will also find manganese supplements online or maybe in probiotic stores.Ask your personal doctor about multi probiotics. You also decide to search the web and locate credible sources. However, remember supplements will not be regulated with the FDA.While probiotics and probiotics can be a necessity, you should consult with your physician before consuming them during the pregnancy or while nursing. probiotics tend not to appear to be they will cause any harm, but you will never know. Taking a supplement without talking to a physician could mean bad things for the health of your son or daughter.Try eating only raw or steamed vegetables. Cooking your vegetables may actually deplete the amount of probiotics within them. If you wish to cook vegetables, steaming them helps keep the probiotics locked in while still providing an excellent consistency. Vegetables that have been frozen continue to be ideal if they aren't overcooked.Your medical practitioner are able to produce a recommendation on which one will suit you better. You additionally opt to look online and find credible sources. There is absolutely no FDA regulating supplements, so that you are all on your own.Zinc is a superb probiotic to help stave off toxins. You enhance your immunity mechanism using this type of trace element, and it assists you to defend against many infections. Zinc likewise helps your system fight against eye diseases, including cataracts, night blindness, and macular degeneration. This important probiotic could be administered orally or topically.Combat the flu or common cold by using a Zinc supplement. You enhance your immune system with this particular trace element, and it allows you to defend against many infections. It's also great for your eyesight. Zinc could be taken topically or orally.Prevent colds and also the flu through taking Zinc. Zinc is an essential trace element that supports good immune function. It is useful in treating ear,nose and throat infections, parasites and malaria. Zinc is additionally good for protection against ocular disease. A few examples include night blindness, macular degeneration and cataracts. You may either get yourself a cream or have a tablet.Combat the flu or common cold by taking a Zinc supplement. It can help battle ear infections, lower respiratory infections, and parasitic infections. Additionally, zinc helps prevent eye problems, for example cataracts, macular degeneration and night blindness. You may take zinc topically or orally.Maintain stocks of fresh veggies in the farmer's market. This is basically the option to finding the freshest produce. When vegetables spend quite a while with packing, shipping and located on shelves, the more depletion of probiotics they experience.Whenever it is possible to, use community farmer's markets to obtain your vegetables. Minimize time a vegetable possibly spends between picking along with your plate so that you can increase the the nutrition it retains. The probiotics will deplete when it just sits over a shelf.Are you now more educated concerning your body? Hopefully you understand how a healthy balance of nutritional supplements is vital. Now that you know how you can improve your health for your better, get out there and undertake it.https://www.smore.com/x30x5-perfect-biotics-but-does-it-work


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Blazer Pro Clamps fit bars with an outside diameter of 33mm-34mm e.g. JD Bug original. For bars less than 33mm a shim is needed . JD bug bars are 33mm so Blazer pro clamps will fit JD bug bars out of the box without the need for the shim. Not suitable for French ID handlebars. Sold individually.
Blazer Double Collar Clamp Black
PLEASE NOTE: We cannot guarantee that these clamps will fit your scooter. View the Clamp Compatibility Guide to see what fits what. If you have any queries, please email us for advice.
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Mobile Workplaces Boost Employee Engagement, Productivity
Six in ten employees said mobile technology makes them more productive, while more than four in ten believe it causes their creativity to rise.There is a measurable link between more mobile-first working environments and an increase in employee engagement, according to a global Economist Intelligence Unit (EIU) study sponsored by Aruba Networks. The study showed that companies rated by employees as pioneers in how they support mobile technology saw a rise in productivity (16 percent), creativity (18 percent), satisfaction (23 percent), and loyalty (21 percent), when compared to organizations that were poorly rated at supporting mobile technology. Six in ten employees said mobile technology makes them more productive, while more than four in ten (45 percent) believe it causes their creativity to rise. The survey also found 42 percent of employees say that the ability to access information quickly and easily has the greatest impact on their productivity levels.
Currently, 54 percent of companies are providing access to the company network from any mobile device to support working anywhere in the office or remotely.
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Exploration reporting
The Chief Government Geologists, through the Government Geoscience Information Committee, have developed a national requirements standard for resources exploration reporting.
- Australian requirements for the submission of digital exploration data (v4.4) - Jan 2017 [PDF 880KB].
Mineral exploration reporting template software
Software to facilitate the creation of geochemical, drilling and other point located data in the required data templates has been developed to assist industry clients comply with the reporting guidelines.
- Mineral Reporting Template (MRT) software v1.4.2 - October 2012 [ZIP 6.8MB]
- Data templates and examples - October 2012 [ZIP 365KB]
Commonwealth reporting guidelines
Offshore minerals reporting regulations can be found on the Department of Innovation, Industry and Science webpage: Offshore mineral legislation and Offshore Minerals (Data Lodgement and Reporting) Regulations 1996 [PDF 31KB].
Further information on offshore petroleum reporting guidelines can be found on the National Offshore Petroleum Titles Authority (NOPTA) website.
State and territory reporting guidelines
Each jurisdiction has its own set of guidelines tailored to its legislation. These state and territory guidelines are based on the national guidelines.New South Wales
- Reporting guidelines
- Exploration reports:
Use SARIG to search the SAMREF database


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luxurious condominium Jolla. One bedroom, one bath with balcony. Includes refrigerator and washer/dryer. Secured underground parking. Includes gas and trash. Prime location. Month-to-month. Call There are: Balcony, Carpet, Community Pool, Community Spa, Dishwasher, Family Room, Granite Countertops, Heading Type, Tile. Copy link - http://ah.qa/98Oy92N to browser to see more details → Read More
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Celebrities & Fan Fiction Series
Celebrities & Fan Fiction Series
CamillaThe ongoing sexual adventures of a nymphomaniac.
Candice Loves Mickie's Fat AssCandice Michelle makes Mickie James her bitch.
Candy HolesCandice has her way with Victoria's ass.
Candy LovingCandy makes the grade.
Carrie Gives an EncoreCarrie Underwood has some fun backstage after a concert.
Carrie Heffernan's Strip DebutTransmission goes out on the road, Carrie takes a 'job'.
Cat TalesCatwoman goes on a heist!
CatsuitSpider-Man is going to bust Black Cat's ass.
CatwomanHuntress joins Catwoman in the sequel to The Eros Formula.


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The Right to Information Bill was passed by the Lok Sabha on 11th May, 2005 and by the Rajya Sabha on 12th May, 2005 and it received assent of the President of India, Dr A.P.J.Abdul Kalam on 15th June, 2005 and has come on the Statue Book as the Right to Information Act, 2005 (22 of 2005). The Act provide for setting out the practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority.
Under Section 15(1) of this Act, it was stated that, "Every State Government shall, by notification in the Official Gazette, constitute a body to be known as the ……..(name of State) Information Commission to exercise the powers conferred on, and to perform the functions assigned to it under this Act". In accordance with this provision, the Mizoram Information Commission, consisting one State Chief Information Commissioner and two State Information Commissioners, was established by the Government of Mizoram on 29th June, 2006 vide Government of Mizoram, Information & Public Relations Department's Notification No.F.13011/11/2005-IPR dt.29.06.2006 and Mizoram Information Commission was officially functioning w.e.f. 05.07.2006. The office of the Mizoram Information Commission, from the inception to till date, is accommodated in the building constructed for All India Services's Transit Accommodation at Khatla, New Secretariat Complex, Mizoram.
Mr. Robert Hrangdawla, IAS (Rtd.) was the State Chief Information Commissioner from 5.7.2006 to 1.2.2010 and Mr. K.Tlanthanga, IB(P)S (Rtd.) was the State Information Commissioner from 5.7.2006 to 30.6.2011.The office of the Mizoram Information Commission, from the inception to till date, is accommodated in the building constructed for the All India Service's Transit Accommodation at Khatla, New Secretariat Complex, Aizawl, Mizoram.
On the selection and recommendation of a Selection Committee consisting of the Chief Minister, the Leader of Opposition in the State Assembly and the Cabinet Minister in-charge of Information and Public Relations Department, Mr. Lal Dingliana, Indian Foreign Service (IFS) was appointed by the Governor of Mizoram as the new Mizoram State Chief Information Commissioner, which was notified vide Government of Mizoram, Information and Public Relations Department's Notification No.F.13011/7/2011-IPR/PT dt. 5th August, 2011. Mr Lal Dingliana, on his taking Voluntary Retirement from Indian Foreign Service and on his return from Venezuela, where he was serving as Ambassador of India, was sworn in by the Governor of Mizoram, M.M. Lakhera on 8th August, 2011 (Monday).
On 21st May 2012 Mr.L.Hrangnawna I.P.S (Rtd) former IGP of Mizoram was sworn in by the Governor Shri Vakkom B Purushothaman as the State Information Commissioner (SIC)
© Mizoram Information Commission (2012). All Rights Reserved


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Publication Name: New Braunfels Herald Zeitung
Location: New Braunfels, Texas
Pages Available: 250,382
Years Available: 1952 - 2013Learn More About This Publication
New Braunfels Herald Zeitung (Newspaper) - March 17, 1989, New Braunfels, Texas Youth showwinners Comal County has the best hogs in the country and Cary Byrd has the best hog at the 21st annual Comal County Junior Livestock Show Thursday night. Byrd, a member of the Smithson Valley Jr. Future Farmers of America, showed the Grand Champion Hog Thursday night during the first event of the three-day show at the Comal County Fairgrounds. Reserve Grand Champion hog • was shown by Amber Heitkamp, a member of the Canyon Junior FFA. SM WINNERS, Pag* 2 Canyon High School FFA member Lilon Holder watches the judge intently as he shows his Lightweight Berkshire Thursday night. A pen of broilers awaits judging this morning at the fairgrounds. Thirty pens of broilers are entered. Making a false report County formulates Renaissance investment policy By STEPHANIE DAVIS Staff Writer A Comal County commissioner is spearheading the enactment of a local policy so the county will better invest its money and comply with the Local Government Code. "I feel that very definitely" the county needs an investment policy, said Precinct 2 Commissioner Neil Craigmile. "There's no reason in my mind that I can justify to myself why we're keeping over $600,CKX) in a checking account." County Auditor Bale Bond pre sented commissioners in February with figures of county checking account balances totaling $762,338. "It's not as cut and dried as it appeared in court," Bond said today. County offices collect money daily and deposit money into the general fund weekly to collect interest, Bond said. As of Dec. 31, 1988, there was $64,937 in the district clerk's checking account; $80,203 in the county court-at-law account; $394,585 in the See PLAN, Page 2 Wholesale prices jump by I percent WASHINGTON (AP) — Wholesale prices soared 1.0 percent in February for the second consecutive month, pushed up by surging energy and food prices, the government said today. The identical back-to-back increases, as measured by the Labor Department's Producer Price Index, mean that inflation at the wholesale level one stop short of retail is running at 12.6 percent annual rate so far in 1989. That is more than triple the 4.0 percent increase for all of last year. Both large jumps had surprised analysts, who in advance of both reports had predicted moderate rises of about 0.4 percent. After January's numbers were reported, economists dismissed them as an aberration and said inflation would return to the 4 percent to 5 percent annual pace of late last year. Wholesale food prices rose 1.2 percent last month, the steepest increase in 13 months. They had risen almost as much in January, 1.1 percent. Most food items were up except eggs, which dropped 15 percent after rising 20.1 percent in January. Food prices were led by huge 35.3 percent increase in vegetables. Energy prices jumped 2.4 percent overall in February, following a 4.9 Se* PRICES, Pag* I Firefighter facing discipline for call By STEPHANIE DAVIS Staff Writer An Austin firefighter faces disciplinary action after calling in a false alarm to local emergency medical technicians while visiting New Braunfels recently. "New Braunfels EMS got a call of a (5-month-old) child locked in a car for more than five minutes daring adverse weather conditions," said Martin Mayer, New Braunfels Police Department spokesman. "Come to find out there was no child locked in the car. The (firefighter) just locked his keys in the car and wanted help from EMS getting them out." One EMS unit responded to the 3:47 p.m. call at the WestPoint Pep- Water legislation faces tough fight By DAVID BUILTA Staff Writer A bill relating to regulation of groundwater in the Edwards Aquifer has not been declared dead for the 1989 legislative session, according to an assistant for Sen. Cyndi Taylor Kricr. A report in today's San Antonio Light said major legislation to enact a regional water plan involving the Edwards Aquifer has been declared dead for the 1989 session by Sen. Frank Tejeda, who sponsored the bill with Kricr. The bill was introduced last Friday. Rep. Jeff Wentworth and Rep. Terral Smith arc the sponsors in the House. Legislation to limit pumpagc from the Edwards Aquifer has been supported by the Edwards Underground Water District — including directors representing Comal County, City of New Braunfels officials, the City of San Antonio, San Antonio Water Board, Greater San Antonio Chamber of Commerce, and Guadalupc-Blanco River Authority. Under the proposed legislation, the Texas Water Commission would have Members of the New Braunfels Renaissance Dance Consort are heading south for the San Antonio Renaissance Fair Saturday and Sunday. The group, which practices at Toby's Dance Studio, will perform four times each day at El Mercado (11:15 a.m., 12:44 p.m., 3 p.m. and 3:30 p.m.) Seated are Jennifer Hoyden and Linda Wesoliek; with, on the center row, Ruth Rouse, Toby Skroder and Julie Livingston; and, back row, Marta Waltman, Carol Gallagher, Marcia Johnson, and Molly Bingham. Persons interested in joining the group and learning the dances may call Skroder. (Photo by Deryl Clark) authority to make and enforce rules and policies. The bill also provides criminal penalties. Residents of Medina and Uvalde counties, joined by others across the state, have expressed ardent opposition to the legislation because they fear the loss of traditional rights to capture unlimited amounts of water for irrigation. Some Bexar County residents also have voiced concerns over the bill. Medina and Uvalde counties withdrew from the Edwards Underground Water District in March because of opposition to a water plan. "I don't think it's dead at all, but it's going to be tough," said Dinah Massie, executive assistant to Sen. Kricr, this morning. "It's one of those things where there are so many people involved." Several lobbyists have been hired to work for the bill's passage. Funds for the lobbying effort have been allocated by the Edwards Underground Water District, City of San Antonio, and San Antonio Water Development Board. District seeking approval After months of intensive self-study, Comal Independent School District in April will be visited by a team of professional educators who will study the district. The 38 educators will evaluate die district's compliance with educational standards established by the Southern Association of Colleges and Schools. CISD made its initial application for membership in the Southern Association during the summer of 1988 then undertook an intensive examination of its schools. Founded in 1895, the association is a voluntary organization which includes more than I LOCK) members. Membership is composed of public schools, universities, professional schools, and colleges throughout the southern region of the United States. "The sole purpose of the SACS is the improvement of education through a systematic program of evaluation according to high-quality educational standards and criteria," said Carol Hall, director of curriculum for CISD. CISD's study involved all teachers, administrators, students and the governing body. Canyon Middle School Priniepal Robert "Rusty" Brockman led the steering committee for the examination. Other steering committee members are Hall, School Board President Carter Casteel, trustee Lee Ikels, and Bm ASSOCIATION, Pag* 2 perch Mill Store Plaza parking lot on U.S.81 March ll. A complaint was signed by EMS technicians and forwarded to Austin Fire Department against the firefighter, Mayer said. "We haven't got all the facts together yet," said Austin Fire Chief See CALL, Page 2Good day It's a nice St. Patrick's Day in New Braunfels. After morning clouds burned off, today's high temperature was expected to reach 81. The low tonight will be 65 degrees. A weak cool front will move through early Saturday. The high Saturday will be 78 and the overnight low will be 58. Sunday will be a little cooler with 71 for the high and a low of 51. Inside: CLASSIFIED...........................10-15 COMICS.........................................8 CROSSWORD...............................3 DEAR ABBY...............................16 ENTERTAINMENT.......................10 HOROSCOPE................................8 RELIGIOUS FOCUS..................6-7 SPORTS.........................................9 TV LISTINGS.................................8 WEATHER.....................................2Stammtisch Members of New Braunfels Marsch-und Wandergruppe invite the public to join them for the Louisa Ervendberg Commemorative Walk Saturday and Sunday. This is the fifth in a scries of spring walks honoring persons associated with the founding of New Braunfels. The award medals depicting these persons were made in Germany especially for this event. All participating paid walkers under IO years old or older than 55 years will receive a gold medal for a 10-kilometcr walk. All others will receive a silver medal for IO kilometers and a gold for 20 kilometers. Pets must be on leashes. The walk, sanctioned by die American Volkssport Association and the Texas Volkssport Association, begins at 8 a.m. at Gruenc Hall. Registration is $5 for Award Medal and IVV Credit ($1.50 for IVV credit only). The route goes dirough Gruenc, into an arca of Preiss Heights, and past the Orphanage on the Guadalupe built in 1846. Water will be available for pets; water and lemonade will be available for walkers at each control point at no charge. The bar at Gruenc Hall will be open as will the restaurants in Gruene. The Commemorative Walk will be held rain or shine. Start at 8 arn. and end at I p.m. (Noon for 20-kilomcter walkers). Finish by 4 p.m. The 10-kilometer walk may be walked twice to obtain credit for 20-kin at no additional charge. For more information, call Florence Riedel at 625-2760 or Hel-gard Suhr at 625-6330. Join the Comal County Extension Service March 27 from 7-9 p.m. for a fast-paced, humorous and educational program at the Bracken Methodist Church in the Garden Ridge area on FM 2252. Certified cooking teachers Lenny Angel and Joan Lyons will feature light and healthy foods using appliances by Osier. They will give all kinds of microwave and cooking lips as well as provide samples. Door prizes will be awarded. There is no fee for the SM STAMMTISCH. Pag* 2 River trespassing bill approved by Senate AUSTIN (AP) — A bill that would restrict trespassing on private land along public waterways was approved by the Texas Senate after critics of the proposal watered it down. See Page 3 Youngsters rewarded during annual show Comal County 4-H, FFA and FHA members worked on their entries this spring and the payoff came Thursday and Friday with the judging and awarding of prizes at the annual Youth Show. Winners will be featured this weekend. _ _ See Sunday's Herald ^ NB nips Georgetown; 410 MO09 10/22/99 SO-WEST MICFOPUBLTSHIHG 2201 BFOOKHOLLOW PLZ SUITE 300 APLINGTON, TX 76006 ley in the Apache tournament. jail tournament :r Georgetown. st round of the -i Smithson Val- See Page 9 Friday March 17, 1989 25 Cents One Section, 16 Pages New Braunfels Vol. 137, No. 90 707 Lands- St., New Braunfels, Texas 78130 (USPS 377-880) 512-625-9144 ;


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Convenient and safe online banking, available 24/7
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As well as being able to access your accounts, view uncleared transactions, pay bills and transfer money, Internet Banking has several other convenient features including:
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To register for Internet Banking or reset your password, please fill out an Internet Banking Enquiry form.
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Where Does Devan Dubnyk Belong in the Oilers' Pantheon of Goalies?
Jonathan Willis
December 12 2013 08:56AM
With the advances in goaltending over the last three decades, it can be difficult to evaluate across eras. But it's possible, by comparing each player to the league average at the time, to approximate how Devan Dubnyk stands up to Grant Fuhr and Bill Ranford and Tommy Salo and all the rest.
Robin Brownlee
December 11 2013 09:49PM
It's easy to dismiss the 7-3-1 record the Edmonton Oilers have strung together in their last 11 games because there's no question this has been a soft stretch in the schedule – assuming you can say there's such a thing as soft when you're talking about a team sitting 14th in the Western Conference.
While I'm not of the mind to shrug off this stretch completely, even with the last win coming by way of a 5-4 victory over the Carolina Hurricanes in a game that turned into a nail-biter when it shouldn't have, I'm not putting too much stock in what we've seen, either.
December 11 2013 05:04PM
There's a lot of talk about young Sam Gagner these days, some troubling moments in the defensive zone married to sluggish offense have many wondering about his future as an Oiler. Should there be concern?
Jason Gregor
December 11 2013 01:53PM
I believe the NHL is much safer now than it ever has been, yet I don't see many articles portraying that. Every time there is a borderline hit, or a spur-of-the-moment dumb decision that results in an injury to an opposing player, some pundits are quick to try and over blow the magnitude of the play.
Since the NHL implemented rule 48, there has been a drop in checks to the head. The players are getting the message.
Brian Sutherby
December 11 2013 12:18PM
"Not all wins are pretty." That phrase is getting used quite a bit today.
Things were looking great when the Oil jumped out to 4-1 lead versus the Carolina Hurricanes. They were in the driver's seat but eventually allowed the Canes back in the game, before pulling it out in overtime.
The Oilers have won 7 of their last 11 games and are far from being a legitimate contender, but at least they are getting some positive results. It was not the second half of the game you want to see but they still won the game.