text:
Sleeping Beautyby Judith Ivory
Once, a long time ago, Coco Wild learned to never allow a man's kiss to move her, for that way led to downfall. A slender beauty with alluring eyes, she built impregnable defenses around her emotions. The men who came to adore her, fought to conquer her and learn her mysteries, made her the toast of London, but none were able to rouse her passions.
Beauty Awakens
Once, a long time ago, Coco Wild learned to never allow a man's kiss to move her, for that way led to downfall. A slender beauty with alluring eyes, she built impregnable defenses around her emotions. The men who came to adore her, fought to conquer her and learn her mysteries, made her the toast of London, but none were able to rouse her passions.
Beauty Awakens
But that hard-taught lesson is about to unravel because of Sir James Stoker. Back in England with a treasure of gold from his adventures, newly knighted by Queen Victoria herself, and feted everywhere, the handsome explorer has the world at his feet. He's exactly the man Coco might have dreamed about when she was young: a tall, fair hero with a charming smile. Now he's turned his energy toward winning her, the most desirable and the least attainable woman he knows; and Coco's aft-aid James may just find his way past the thorns that have so long protected her from the world, and awaken her slumbering heart...with just one kiss.
Editorial Reviews
Product Details
- 9780380786459
- Publisher:
- HarperCollins Publishers
- Publication date:
- 06/01/1998
- Series:
- Avon Romantic Treasure Series
- Pages:
- 384
- Product dimensions:
- 4.18(w) x 6.75(h) x 0.96(d)
Read an Excerpt
The light in the room grew hazy with the passing of a cloud outside. The incoming sunlight became muted, dust motes dancing visibly in the beam that poured through the windowpanes. Mrs. Wild turned toward him slightly, sitting crookedly in her chair, raising one elbow leisurely up onto her chair back. She sat on her discarded coat.
James meant to bring the conversation back to where it belonged, into the realm of inane chatter, niceties, all unpleasantness minimized.
But she said, "I think the Burtons and Livingstons of the world quite mad to pursue so much danger, let alone discomfort."
Exactly. James felt a little zing of rage, injustice. He found himself adding, "It's almost galling, you know." A kind of confession poured out. As if he were under the influence of laughing gas, giddily telling a stranger the truth. "A chipped tooth. It hardly seems worthy enough damage, considering I was at wit's end, lost, sick, and sure of dying in the midst of people to whom I could barely speak. I mean, I should be missing a leg, an arm, be wearing an eye patch. But no. Not even a scar. I am healthier and stronger than the day I left England. With nothing to show for three and a half years of hell but a tooth I banged on something or other on one of the numerous occasions when I blacked out and fell flat on my face." He let out a snort of disgust. "And within the hour, even that will be fixed" He took a breath.
God, give him enough time and he would tell Mrs. Wild here his life's story.
His mouthful was hardly the heroic tack, hardly the vein in which he, the Queen, and the Vice Chancellor spoke of these things. James had not told his true feelings to a soul. Not even himself,apparently. For he was mildly appalled to hear these wordsand feel the extreme satisfaction of saying them. They were so true. And here he was, pouring himself out to an unknown woman in a dentist's waiting room, just because she seemed to recognize the truth when she heard it and have some sympathy for it. Just because she had a pretty face and an understanding mien.
He pressed his lips together, not sure what he had doneother than discomfit himself considerably.
She smiled at him and leaned forward. Again the light touch of her hand over his, again quickly with drawn. "Don't worry," she said. As if she could read his mind now as well. "I won't mention it. I can see that you have embarrassed yourself by speaking so openly. But believe me, there is nothing wrong with being open. Everything right with it, in fact." She waved her hand as if she could wave away his concern. "And your secrets are safe with me. Honestly. I have no one to share them with. And even if I did, I never tell tales. It's unseemly, don't you think? To go around telling one person what another one told you? I mean, if a person had wanted someone to know, he could have said him self, no?"
James said quickly, "You are so right. Thank you."
She nodded, her mouth pulling into a smiling line, just the faintest humor. Then she held up her new silver case of cloves. "My pleasure," she said, repeating his words, indicating an exchange.
Rightly or wrongly, James felt his distress ease. Apparently, a cozy dentist's parlor could be a haven for a few minutes, a place to babble in safety. For that was certainly how it seemed.
The strangely charming Mrs. Wild stood. From her chair James picked up her coat, a loose, silky bundle of dense fur. He held it for her. It was not bulky, but rather the well-trimmed, feather-soft undercoat of a winter animal. The label sewn into the satin lining bore a name: Wonh. And a city: Paris. The coat perfectly matched the fur at her cuffs and collar and the small hat that sat back on her head. The hat's nettingstudded at each juncture with tiny bits of cut jethad been folded back, presumably so she could have her good, eye-mopping cry. The net sparkled with these bits.
James stared down at it as he helped her, an arm at a time, into her coat. A coat sublimely soft in his hands, the lovely weight of smooth, glistening fur that poured all the way to the floor. It was really a gorgeous piece.
As was the woman who smoothed her gloved hands down the front of it, then pulled the coat's collar to her throat, her fists tight about its edge.
On a whim, James said suddenly, "I have a party to go to tonight. In my honor, actually. I would be delighted if you would accompany me."
She glanced up, lifting one eyebrow. Her gentle smile became arch, faintly amused, faintly skeptical. She said, "You are a lovely young man."
The operative word was young.
"A dear," she added. She smiled again, less kindly.
All right, he had overstepped. It had been overly familiar for him to ask such a thing. As if he could pick her up off the street. But James found himself, since returned to England, not always in harmony with the rules of protocol and society. The same rules he had lived by four years ago occasionally struck him now as stupid, arbitrary. Why not? Why couldn't he ask a charming woman to go to an enjoyable celebration, and thus make it more enjoy able?
She said, "You must find yourself a nice young woman to go with you to your parties."
She reached above her head and lowered the netting of her hat over her face. For an instantfrom behind the bobbinet, as her eyes held hisit seemed to be something more, something beneath all her chic and polite smiling: a flicker of indefinable sadness. Then it was gone.
"Thank you," she said again. Whatever James had seen, if he'd seen anything at all, she recovered quickly. "Please tell Mr. Limpet that I have decided to keep my terrible tooth a while longer." She laughedonce more the treat of that whisper-like sound. "I am just too attached to it, tell him. I can't give it up just yet. Maybe tomorrow. Maybe never. Thank you again, Mr. Stoker."
And, with a sway of sliding fur, the lovely Mrs. Wild turned and disappeared through the haze of motes and sun out the door into a bright spring afternoon.
Meet the Author
Judith Ivory's work has won many honors, including the Romance Writers of America's RITA and Top Ten Favorite Books of the Year awards and Romantic Times Reviewers' Choice Award.
Customer Reviews
Average Review:
Most Helpful Customer Reviews
See all customer reviews
James Stoker a young Cambridge professor/geologist/explorer, falls in love with an older more experienced woman, a soiled dove, if you will. She is at first put off by his youth but he's such a handsome, thoroughly male, earnest and open fellow she just can't resist for long. James and Coco fall in love but have to hide their relationship as Coco's reputation could ruin James' career. There is treachery afoot as one of her former, lovers, from long ago, frames James, out of jealousy, for crimes he didn't commit. Of course everything comes aright in the end though it seems there is no way that can happen. The protagonist in the book is truly despicable and I found myself wanting to throttle the chap. The language in the book seems typical of the era. I find this makes a book more believable. There are some very steamy love scenes so if you're a prude this book is not for you. I have ready many, many romance books in my 63 years and I know a good book when I see one. Judith Ivory is genius. I loved "The Proposition" which, I highly recommend. I think would also be a great movie. I wouldn't say that about many books I've come across.
very dissapointing


text:
Here is a look at the division leaders in some key statistical categories after Week 6:
Passing yards
Player: Peyton Manning, Denver
Yards: 1,808
Comment: Manning is putting together MVP numbers.
Rushing yards
Player: Jamaal Charles, Kansas City
Yards: 591
Comment: Charles is first in the NFL in this category.
Player: Demaryius Thomas, Denver
Yards: 542
Comment: Thomas is making a Pro Bowl push.
Player: Derrick Johnson, Kansas City
Total: 50
Comment: He is a steady force in a miserable season for the Chiefs.
Player: Justin Houston, Kansas City; Von Miller, Denver
Sacks: 6
Comment: This will be a fun race to follow.
Player: Several are tied.
Interceptions: 2
Comment: This logjam will eventually sort itself out.

subset: commoncrawl


text:
Top Definition
tattoo above a woman's crack, lower back tattoo, tramp stamp, hoe tag, whore tag, slut, a woman you gets used a lot for sex.
that girl has a crack tattoo, she must be the easiest girl in the bar tonight.
by nelly4alluhoes February 06, 2015
The Urban Dictionary Mug
One side has the word, one side has the definition. Microwave and dishwasher safe. Lotsa space for your liquids.Buy the mug
The Urban Dictionary T-Shirt
Smooth, soft, slim fit American Apparel shirt. Custom printed. 100% fine jersey cotton, except for heather grey (90% cotton).Buy the t-shirt


text:
With Instructables you can share what you make with the world, and tap into an ever-growing community of creative experts.
From today I am sure that many of you will start home making their own butter. Why? 1. Because it's easy 2. Because it's fast 3. Because it's ...
Pour the heavy cream in a bowl of your electric mixer, turn it on high speed and whip up the cream. When the cream it's nice and whipped...don't ...
Join 2 million + to receive instant DIY inspiration in your inbox.
© 2016 Autodesk, Inc.


text:
I've never heard of this before but it looks awesome and very helpful during the upcoming summer months!
Right now you can get the for only $31.65! That's nearly 50% off the normal price of $59.95!
- Bluetooth for wireless audio streaming from your
- Sealed, top-mounted controls for easy access and weather resistance
- Play/Pause, Track Change, Volume and Hands-free controls
- Splash-resistant for all of your outdoor activities
- Cylindrical design fits most cup holders for convenience
Remember that Amazon can and will change prices at anytime!


text:
Foxley's Gallery Ltd.
Art & Framing Store. Original artwork by local and regional artists. Dedicated space for our gallery art shows. Serving the Fox Cites for over 25 years. Over 4,000 prints in stock & selection of frames.
Hours of Operation
Mon-Fri 10am-5:30pm Sat 10am-3pm Sun Closed
Shopping Type
- Specialty Shop


text:
Hostelworld anbefaler
Vi elsker dette vandrerhjemmet! Vi gir tommelen opp for gjennomgående gode kundekarakterer og utmerkede kvalitetsvurderinger.
Sammenlign resultater
Når vil du være i Peniche?
Angi datoer for å sjekke tilgjengelighet
- Søk etter tilgjengelighet og priser
- 20 Feb 2017, 3 Netter
- 2 Gjester
- Endre Søk
- Pris
- Rangering
- Avstand
- Navn
- Vis:
- Liste
- Galleri
Residencial Aviz
We offer several options, from simple rooms with shared bathroom to rooms for up to 4 people, with private bathroom, Television . We also have free wifi available.
Whether you are a backpacker or a... Mer...
- Pris Romtype
Surfing Baleal
WCT Rip Curl Pro
Supertubos - Peniche - Portugal
Based about one hour from Lisbon and 150 metres from the beach, this establishment offers everything from accommodation, camps, rentals, tours, su... Mer...
- Pris Romtype
Peniche Shoreline Guides
Peniche Shoreline offers travelers everything needed for a perfect vacation:
- comfortable accommodations,
- fully-equipped kitchen,
- cozy living room with satellite TV, DVD and surround soun... Mer...
- Pris Romtype
Peniche Guest House
Peniche Guest House, is a traditional house from a local family, the house is very well located , in one of the best nature places of Peniche.The kitchenette is full equipped, and it has seats for all... Mer...
- Pris Romtype
Baleal Cliff House
Baleal Cliff House is nothing less than its name, our beloved ocean view B&B is your best choice between a cosy, relaxing holiday a stone's throw away from the beach and a surfing trip next... Mer...
- Pris Romtype
Peniche Beach House
We have several types of accommodation: private rooms, some with balcony with sea view, shared rooms, with bunk beds (maximum 4 persons) perfect for groups or for relaxed people who like to share lod... Mer...
- Pris Romtype
Villa Berlenga
Situated in a quiet village with views over the island Berlenga, Berlenga Villa Holidays offers bright rooms, a garden and barbecue facilities only 4 km from the beaches of Baleal.
Located between... Mer...
- Pris Romtype
Hostelworld mobilapper
Bestill mens du er på farten med de nye mobilappene til Hostelworld.


text:
On the other hand, those churches which teach that the Bible is the true and inerrant word of God find their congregations grow. People search for truth, and when they find it are unlikely to let it go.
High Wycombe, Buckinghamshire
- More about:
- Bible
- Buckinghamshire
- Churches
- High Wycombe


text:
This is a great post for guys who are about to pop the question and need a really spectacular ring. Whether you're just getting ideas and need inspiration or you're ready to purchase the ring that really stands out to you, you're going to enjoy this. The following stunning rings are our favorite engagement rings at jamesallen.com.
1) Mille Coeurs 18k White Gold 1.22 CT Round Blue Sapphire Engagement Ring
This gorgeous piece of jewelry is so beloved because it's different from the traditional diamond engagement ring, and every woman wants something unique. It features an infinity basket design and pave halo around the sapphire for ultimate sparkle level. This is for the romantic woman, the girl who loves sentimental love letters and saves movie tickets from all of your dates.
2. Royal Halo Cushion Shape Classic Engagement Ring
This beautiful ring features a cushion-shape halo with a princess-cut diamond in the center, and a pave design on the band. It has a vintage look, and at just over $2,000 it's totally affordable. This is for the girl who cherishes old movies, has her grandmother's vintage linens and daydreams often.
3. Royal Halo Classic Engagement Ring
This beautiful and classic-style engagement ring features a 1.75 carat diamond in the middle of a royal halo. This one is really perfect for the traditional couple, and it's a steal at under $3,000. This is the perfect ring for the modern girl; someone who loves her Starbucks coffee or green tea, wears heels with jeans and won't pass up a sale at her favorite store.
4. Tuscan 18k 3 Tone 1.08 Carat Yellow & Pave Diamond Ring
This pure piece of beauty features stunning diamonds in the center and to each side, surrounded by pave halos. It's a very ornate piece of jewelry and still very affordable for under $5,000. This immaculate ring is for the girl who somehow manages to do it all and make it look super easy to the rest of us!
5. Design Your Own Ring Option
We couldn't leave out the custom rings at James Allen because they allow couples to custom-create a ring that is perfect for them. When you really want something that suits your style and taste, there's nothing better than something you designed yourself. More and more couples are opting for custom-created rings in order to ensure that no one else has a ring like theirs.
These are just a few of the simply stunning choices available for grooms who are currently shopping for the ring so they can ask the question to the girl of their dreams!


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I am looking for help
If you have no idea where the money is, you are not going to be terribly successful. If there was a probate you should check the probate file for details, and that should describe what the assets were and how they were distributed. If there was no probate, but there was a will -- who was named as the personal representative? That person may have more information. If your father had a 'family attorney' you could try to call that person to see if there are records.
Short of that, you can search for 'escheated funds' in any state / province where the money MAY have been, but that can be a complicated and drawn out process in multiple jursiditctions. Good luck!
When you say "left some money" that implies there is an instrument of some kind that gives you the right to the assets. There are asset finders which you can find on the internet who will do traces for assets, but it will cost you either up front or as a percentage. You can also check jurisdictions where he lived for escheated funds (went to or is held by the State or Province). It is a laborious process but if you have some inkling there is something out there you should pursue it.
This comment does not constitute the giving of legal advice nor create an attorney client relationship.
To get a good answer to your question more facts are needed. How do you know he left some money for you? When did you learn of this? Where did he live? Do you have access to his paperwork?
Although the above response is believed to be accurate, it should not be relied upon as any type of legal advice because the information provided is incomplete. It is intended to educate the reader and a more definite answer should be based on a consultation with a lawyer. No attorney client relation is formed with me without a written contract.
Good Luck starts with a strategy and a plan.
Robert J. Suhajda, MS,CPA
Attorney At Law
17721 Norwalk Blvd. #43
Artesia, CA 90701
Former financial auditor and controller. Admitted to US Tax Court, Income Tax, IRS representation, Fiduciary income tax returns, Estate and Gift tax returns,
Homeowner Association Strategist.
Post a free question on our public forum.Ask a Question
- or -
Search for lawyers by reviews and ratings.Find a Lawyer


text:
The Silver Palate Cookbook "Silver Anniversary Edition" by Julee Rosso, Sheila Lukins and Michael McLaughlin, now includes color photos of many of the classic recipes from the original. Don't miss Chicken Marbella, Beet and Roquefort Salad with Walnuts, and Salmon Mousse.
Makes at least 12 portions
This has become a Silver Palate classic. It was with us the first day we opened and the only time that it is not in the refrigerator on any given day of the year is when we've sold out. It is light, pretty, refreshing and one of those foods that you enjoy time after time.
1. Soften the gelatin in the cold water in a large mixing bowl. Stir in the boiling water and whisk the mixture slowly until gelatin dissolves. Cool to room temperature.
2. Whisk in the mayonnaise, lemon juice, grated onion, Tabasco, paprika, salt and dill. Stir to blend completely and refrigerate for about 20 minutes, or until the mixture begins to thicken slightly.
3. Fold in the finely flaked salmon. In a separate bowl, whip the cream until it is thickened to peaks and fluffy. Fold gently into the salmon mixture.
4. Transfer the mixture to a 6- to 8-cup bowl or decorative mold. Cover and chill for at least 4 hours.
5. Serve on toasts, black bread or crackers. Or serve as a first course, garnished with watercress.
Buy The Silver Palate Cookbook
This page created June 2007
The FoodWine
Main Page
Kate's Global Kitchen
Kate's Books
Cookbook Profiles
Global Destinations
Holiday & Party Recipes
I Love Desserts
On Wine
Caffeine and You
Cooking with Kids
New Green Basics
Conversions, Charts
& Substitutions
About FoodWine
Contact Info
Privacy Statement
Anatolia: Turkish Recipes
Bar Tartine
The Beer Bible
Beetlebung Farm Cookbook
Bird in Hand (Chicken)
Bitter: Flavor
Bob's Joke Burgers
Cooking Light
Darjeeling Tea
Dinner at Home
Everyday Baker
Fast Food (Andrew Weil)
Flavor Flours
Food 52 Genius
The Food Lab
Garden Recipes
Grandmas' Recipes
Hartwood: Yucatán
Humane Butchering
Heritage Southern Recipes
Jemima Code African Recipes
Kitchn Cookbook
Liquid Intelligence
Near & Far World Recipes
NOPI Restaurant Cookbook
Oxford Companion to Wine
Phoenix Claws: Chinese
Picnic: Recipes
Soda Politics
Southern Recipes
The Third Plate
V Is for Vegetables
Vegetarian India
What Katie Ate
The Whole 30
Whole Food Kitchen
Yucatán Recipes
Zahav Israeli Cooking
Copyright © 1994-2016,
Forkmedia LLC


text:
Kathleen Matthews Md, Physicians & Surgeons, listed under "Physicians & Surgeons" category, is located at 501 S Cherry St Denver CO, 80246 and can be reached by 3035727578 phone number. Kathleen Matthews Md has currently 0 reviews.This business profile is not yet claimed, and if you are the owner, claim your business profile for free. If you are not the owner you can invite this business to join by email or by phone.Browse all Physicians & Surgeons in Denver CO. Discover census data for Denver, CO.
Back to Top


text:
Selecting a Roofing Specialist for Your Residence
When you find you require roof repair in utah, you may freak out. The cost of a whole new roof isn't low cost, however this is one job you cannot afford to defer, as the roof performs a vital role in the total worth of your house. Your main goal is to locate roofing specialists in utah that can help with your roof repair without considerably hurting your wallet. How do you approach finding a business providing reroofing in utah which will not overcharge you, but will do the job correctly? When looking for a roofing company, you must be sure that you require recommendations. Your goal is to ensure the project was finished properly at the arranged amount and also in a timely manner. Ask about customer support when you first speak to former clients so that you understand what can be expected. On top of that, make sure you ask for an itemized quote from each company so you can evaluate the estimates in terms of products, labour to be carried out and more. In no way base your decision on which professional supplies the cheapest price either. Not merely could this mean they may be using low quality items or craftsmanship, they may not be still operating in the event that an issue develops in the future. You would like to ensure the company has got the appropriate licenses and insurance for the job, therefore you understand that you are protected if something really does go wrong and make sure to check out any kind of warranty they have on products and labour. Every roofing shingle manufacturer carries a guarantee for their goods, however, some can be better than others. Be sure you carefully review the fine print of these extended warranties so there are not any unpleasant predicaments later on. Take some time together with the contractor too. They are going to be doing work at your home near your loved ones. You need somebody who will certainly handle your loved ones with respect and will not be cigarette smoking or alcohol consumption while on the project. Language ought to be worth focusing on as well. Should you talk to a contractor and have a bad feeling about her or him, continue looking. It isn't worth keeping a few dollars to be not comfortable with the roofer that you have employed.


text:
More Videos...
Blown Nova Goes 6.39 on 10.5 tyres
Frank Mamone's blown Chevy Nova is a wicked looking beast and it's got plenty of grunt to match. Lately he's been racing the Hemi powered Nova inn the 10.5 class and with some success too. He won at Sydney Dragway recent at the APSA Pro Street Shootout.
KRANKY Burns Down Kandos - Burnout Fire!!
We've seen plenty of burnouts end with a fire but we've never seen one start with a fire. Rodney Waters HQ sedan goes up in flames right from the start at Kandos. It's crazy insane.
Summernats 25 - Day Three
Having trouble loading this file up. hopefully this time it works. Okay guys, I finally got this to load up. All the action from Friday with Dyno, burnouts, unveiling, bands and plenty of cruising. What a huge day!
Car's differential explodes on the startline
This supercharged Torana blows its diff in spectacular fashion during Supernats at WSID in Sydney Australia.


text:
Welcome to Zopper! You seem to be near Aaya Nagar, Delhi. If your delivery location is different, please change it above. X
Creative Inspire T6100 5.1 Multimedia Subwoofer
Warranty Period:
1 Year Manufacturar Warranty
Speaker Power Output:
76 Watts
Out of Stock
• Free Shipping
• 100% Genuine Product
• Cash on Delivery
• 10 days replacement
• Delivery in 2-3 business days
Be The First One to Write Review
Write a Review
Creative Inspire T6100 5.1 Multimedia Subwoofer
Product Details
Warranty Period
1 Year Manufacturar Warranty
Items in the box
Home Theater, User Manual, Warranty Card
Add on Features
Other Features
Flared port for lower and cleaner bass response, More power and depth, Easy controls for private listening
Speaker Features
Audio Compression formats
Mp3 File Format
Speaker Power Output
76 Watts
Speakers Configuration
5.1 Channel
Nearby Localities
Enter your mobile number


text:
- {{matchup.date.fullday}}{{matchup.date.month + ' ' + matchup.date.day}}
Cavaliers overpower Grizzlies
Daniel Gibson dropped 19 points off the bench, as the Cleveland Cavaliers got past the Memphis Grizzlies 94-79 on Tuesday night.
Cleveland cashed as 14.5-point home favorites as the game played under the 188.5-point total posted by oddsmakers.
Mo Williams added 18 points and six assists for the Cavaliers, who got 15 points and eight assists from LeBron James in the win.
Rudy Gay and Hakim Warrick scored 13 points each for Memphis, while Mike Conley went for 12 points and seven assists for the Grizzlies.


text:
The table below looks at the statistics for TTCN skills in IT jobs advertised across the UK. Included is a guide to the salaries offered in IT jobs that have cited TTCN over the 3 months to 20 February 2017 with a comparison to the same period over the previous 2 years.
The following table is for comparison with the above and provides statistics for the entire Programming Languages category in permanent jobs advertised across the UK.
The demand trend of job ads citing TTCN as a proportion of all IT jobs with a match in the Programming Languages category.
This chart provides the 3-month moving average for salaries quoted in permanent IT jobs citing TTCN within the UK.


text:
Coast to Coast Tickets is the place for great deals on concert, sports and theater tickets!Follow @CTCTickets
|Brown Center
|Piscataway, NJ
|High Point Solutions Stadium (formerly Rutgers Stadium)
|Piscataway, NJ
|Louis Brown Athletic Center
|Piscataway, NJ
Coast to Coast Tickets is the place for great deals on concert, sports and theater tickets!Follow @CTCTickets
If you would like to attend a concert in Piscataway to experience the live music scene, Coast to Coast Tickets is your source. All it takes is a phone call or a few clicks of the mouse to purchase concert tickets for Piscataway and the surrounding areas.
Piscataway Concert Tickets
Whether you prefer a drama, comedy, or musical, we will provide you with seats to the hottest live theater preformances. Coast to Coast Tickets is your ticket broker for all theatre events in the Piscataway area.
Piscataway Theater Tickets
Enjoy live sporting events in Piscataway! Coast to Coast Tickets offers tickets for all professional and college sports, including baseball, football, basketball, hockey and more. We carry tickets for all venues, such as stadiums, amphitheaters, clubs, racetracks, and arenas.
Piscataway Sports Tickets
Sign up for email updates


text:
"Hi Mary, I'm looking for someone to practice with as well. I have a permit, so I can play anywhere. Weekends and Wednesdays work best for me. thanks, Amy "
556 members17 Likes
a scan has not received any gifts yet
© 2017 Created by Mark / The Mayor. Powered by


text:
No products
Check out
Viewed products
This product is no longer in stock
Availability date:
Tweet Share Google+ Pinterest
Baccarat Toile unie speciale nappe grande largeur: 300cm noir Thevenon 1526629 le metre
Recipient :
Name of your friend * :
E-mail address of your friend * :
* Required fields
Send or Cancel
Add to wishlist
This product is not sold individually. You must select at least 1 quantity for this product.
Add to cart
No customer reviews for the moment.
* Required fields
Submit or Cancel


text:
New Release & Not Available In Stores. Printed in the USA.
Are you obsessed with your cats? This Tees is perfect for you.
Made of 50/50 ring spun combed cotton/Polyester which makes it Durable, Lighter and Softer than basic cotton tees.
View Sizing Chart
Click "Add To Cart" Button to order now!
Extra Information:
Estimated Delivery time : 5-12 Days (USA) & 5-18 Days (International delivery)
All prices are quoted in US (USD) dollars
Safe Checkout To Credit Card & PayPal


text:
More Videos...
Telling a lifted truck owner he has a small dick
When keyboard commando's forget they are not behind a computer. Would you tell a lifted truck owner he has a small dick out loud and in person?
Dads reaction to twin turbo duramax
Jeremiah 29:11-13 11 For I know the plans I have for you," declares the Lord, "plans to prosper you and not to harm you, plans to give you hope and a future. Then you will call on me and come and pray to me, and I will listen to you. You will seek me and find me when you seek me with all your heart.
Cold Start Cummins 5* below
24v cummins sled puller. Custom ground haisley cam, 200 hp ddp injectors 64/71/14 turbo, port and polished head, fly cut pistons, quadzilla adrenaline stacked with a smarty.
dodge ram 1500 exhaust stacks magnaflow 4" race muffler
So I got pulled over last weak for being to loud so I decided to try a slightly bigger muffler. Its a magnaflow round race muffler # 14163. Now this is first start up and it still needs the 1200 mile break in so its pretty quiet. Ill make a new vid once its broken in. 2001 dodge ram 1500 5.2 5 speed 5" lift 4" magnaflow race muffler # 14163 4" stacks 3.5" magnaflow cat super swamper ssr's 33/14.5 r16 ebay cold air intake mickey thompson classic 2's


text:
More Videos...
Truck-Re-Birth of a Monster
Volant Intake and Exhaust on 2007 Toyota Tundra 5.7L V8
2010 Tundra Crewmax New Volant Air Intake
Before and after volant air intake install. Sounds awesome! Definitely can feel a better throttle response when you gun it its instant power and doesn't hesitate at all like it would before. 0-60 feels a little faster but once past 60 it really pulls a lot harder then it use to!
2014 Tundra Flowmaster 50 Series and AFE Stage 2 CAI
Clips of the 2014 Tundra
2010 Toyota Tundra 5.7L iForce V8 On The Dyno
Mods: Afe Cold Air Intake System, MBRP True Dual Exhaust System, and Custom Tuning Made: 330 WHP, 350 LB/FT


text:
25 Easy to Make Homemade Jewelry Crafts
Bonus: Get our newsletter & special offers for free. We will not share or sell your email address. View our Privacy Policy
Latest Comments
"I like this vintage style Santa graphic, but I read that the designer used a cutting machine to cut a red vinyl decal to afix to the cream painted wood board. I don't know what kind of cutting machine she is talking about and I wonder how pricey it is? I'd like an alternative to getting that Santa on there."
"This wall decor is very chic with any message you write on it! And it couldn't be easier. However, another option to using foam core board would be to actually buy an old thrift store bulletin board and spray paint it black. I think it would give the wall art a little bit more of a lived-in rustic look as the cork board adds texture and interest."
"This DIY holiday project is something that I would like to do at home myself. This craft is not for everyone's taste, but if you like a certain minimalist decorating approach and you've got a creative eye then this is for you! At first I did not even notice the picture frame in the background. It's used to attach the branch to it. Cute idea!"
Connect-the-Dots Bronze Pearl Earrings
By: Keri Lee Sereika for Pink Lemonade
We are adding the craft to your Craft Projects.
The project was added to your Craft Projects.
Click here to view your Craft Projects.
These "connect-the-dots" bronze pearl earrings are so simple to make and are sure to attract attention. Keri Lee Sereika takes a twist on classic pearl earrings with this zig-zag dangle effect. Sophisticated yet fun! Try different colored pearls or beads too!
- Six headpins (Here I have antiqued brass)
- Six beads of your choice (Here I have bronze pearls)
- Pair of matching earwires
- Round-nosed pliers
- Chain-nosed pliers
- Flush cutters / wire nippers
- Thread pearls onto bottom of headpins.
- Use wire snippers to trim about 1/2 inch from top of one pair of headpins.
- Use chain-nosed pliers to bend headpin approximately 3/8 inch from top.
- Use round-nosed pliers to turn loop at top of headpins.
- Thread second pair of headpins through loops. Repeat steps 2-4.
- Repeat step 5 for final pair of headpins.
- Use chain-nosed pliers to twist bottom of ear wire loop to open; place top of earring loop in open ear wire loop.
- Use chain-nosed pliers to twist loop on bottom of ear wire closed.
- And here's your final project! Enjoy!
Tags / Related Topics
Your Recently Viewed Projects
Images from other crafters
Love these projects? Get MORE delivered to your Inbox – FREE Click Here!
Apr 30, 2016
These earrings look like they would be so hard to make but so super easy but so elegant looking. I like the unique design. So different then any earrings I have seen. I am adding these to my collection and will be making some as gifts. This tutorial is done that making these earrings should be no problem. Anyone new to this try this/
dunder 4818730
Apr 17, 2013
These are really cute. I definitely want to try this!
Report Inappropriate Comment
Are you sure you would like to report this comment? It will be flagged for our moderators to take action.
Thank you for taking the time to improve the content on our site.
Something worth saving?
- SAVE all your favorite crafts
- ADD personal notes
- QUICKLY reference your crafts
Free eBooks
- 11 Crochet Shawl Patterns: Crochet Poncho Patterns, Free Easy Crochet Patterns and More
- 12 Knitted Scarf Patterns: Fabulous Free Knitting Patterns for Beginners
- 14 Free Crochet Patterns for Babies and Toddlers
- 16 Free Crochet Hat Patterns, Scarves, and Gloves
- 22 Free Crochet Patterns: Afghan Patterns, Crochet Hats, and More
- 24 Quick and Easy Knitting Patterns
- 7 Adult Coloring Pages
- 8 Free Apron Sewing Patterns
- Easy Lace Knitting Patterns
- Quick & Easy Decoupage: 12 Fabulous Mod Podge Projects for Your Home
Our Newest Projects & Articles
- 11 Easter Crafts for Kids
- 100+ Easter Crafts
- Duo of Bears Unisex Baby T-Shirt
- Cross My Heart Treat Bag
- How to Sew a Zipper into a Lined Skirt
- Tiny Valentine Gift Boxes
- Pretty Hearts Hot Pad
- DIY Mini Pallet Photo Magnets
- Padded Denim Hearts for Decoration or Keyring
- Faux Topiary Heart Place Card Holders


text:
I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.
4-(2-Hydroxyethyl)pyridine acts as a reagent for the protection of carboxyl groups as their 2-(4-pyridyl)ethyl esters in organic synthesis. Further, it is used as an active pharmaceutical ingredient intermediate.
Reagent for protection of carboxyl groups as their 2-(4-pyridyl)ethyl esters, by reaction with the acid chloride: Tetrahedron Lett., 25, 1223 (1984). See preceding entry under 2-isomer. See also 4-Vinylpyridine, L13316.
Kato, Y.; Kawasaki, M.; Nigo, T.; Nakamura, S.; Fusano, A.; Teranishi, Y.; Ito, M. N.; Sumiyoshi, T. Identification of 2,3-disubstituted pyridines as potent, orally active PDE4 inhibitors. Bioorg. Med. Chem. 2013, 21 (18), 5851-5854.
Aschenbrücker, J.; Steegmüller, U.; Ernsting, N. P.; Buback, M.; Schroeder, J.; Jasny, J. Picosecond UV pump/IR probe spectrometer for the 2-5 mum spectral region. Appl. Phys. B: Lasers Opt. 1997, 65 (4), 441-444.
Hazard Statements: H315-H319-H335-H227-H303
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation. Combustible liquid. May be harmful if swallowed.
Precautionary Statements: P210-P261-P280-P305+P351+P338-P304+P340-P362-P312-P405-P403+P233-P501a
Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.


text:
Position Announcement: Academic Fellowship in the Critical Race and Ethnic Studies Program at the University of Vermont College of Arts and Sciences, Andrew Harris Fellowship Program.
The Department of Gender Studies, Faculty of Arts and Science at Queen's University, Canada invites applications for a Tenure-track faculty position at the rank of Assistant Professor with specialization in Critical Race, Gender and Sexuality Studies in Transnational Contexts, with a preferred starting date of July 1, 2015, subject to budgetary approval. The deadline for applications is February 12, 2015.
We are seeking papers for a collection of academic essays at the intersection of critical race theory and ecological criticism. Ideally, papers will engage the semiotics of race and nature, identity and place, culture and economy, and/or history and the future. To reframe Donna Haraway's foundational critical questions in /Primate Visions/, we ask how do "the marks of race and [difference]" enable or disrupt ecocritical and cultural knowledges and possibilities in a "post racial," late industrial society? How can we theorize and productively respond to the impact of racial formations on the environment, historically, and in the age of the Anthropocene? Our collection will draw on the long-standing critical and environmental activist work in Native American studies and on traditions of ecofeminist scholarship. Deadline to submit is January 12, 2015.
Conference Submissions Deadline EXTENDEDSubmissions deadline for Sovereignties and Colonialisms: Resisting Racism, Extraction and Dispossession as been extended to Friday, December 19th at 5pm EST
The Ethnic and Women's Studies Department at California State Polytechnic University, Pomona invites applications for a tenure-track position at the rank of Assistant Professor to begin Fall 2015. We seek a candidate with expertise in African American Studies.
Stay informed on our latest news! We'll periodically send you emails on news, and conference related updates.
Donate to support CESA scholarship funds, programs, and events.


text:
The MA program in Classics at Memorial offers students the opportunity to expand and deepen their knowledge of ancient Greece and Rome. The department is able to provide a flexible course of studies that caters to the individual needs and interests of students. Specific areas of strength are Roman Studies, including literary and cultural studies; Greek literature and historiography; and more broadly, the relation between literary texts and material culture within the various subdisciplines of classical studies.
Graduate applicants interested in research-based programs are strongly encouraged to contact a faculty supervisor at the time of application. Visit the Department of Classics website for a list of faculty members, their research interests, and contact information. You may also use the Yaffle search engine to find a supervisor you may wish to work with.
Important Note: The information provided on this website is subject to change. The University Calendar is the final authority on university fees and regulations.
MA: 2 yearsCampus: St. John's
January 31 for Fall admissionFunding:
MA: $632-$953/semesterTuition (International students):
MA: $822-$1,239/semesterApply


text:
ADMINISTRATIVE CORE for Vaccination and infection: indicators of immunological health and responsiveness. The Administrative Core of the Stanford Human Immune Monitoring Research (SHIMR) Center will oversee the conduct of the research projects, pilot projects, and scientific cores proposed here. The Administrative Core will be led by Dr. Mark Davis as Project Leader, with Drs. Ann Arvin and Harry Greenberg as co-Project Leaders;they will anchor the Executive Committee, with the other Project Leaders Drs. Goronzy, Maldonado, Gans, Utz, Koller, and Fire as members. In addition, two other senior investigators from Stanford's School of Medicine will be recruited to serve on the Executive Committee on a rotating basis for two year terms.
The Specific Aims ofthe Administrative Core are to: 1) Implement administrative &leadership mechanisms that will facilitate communication and cooperation among the Stanford project leaders and with the consortium and investigators at other institutions to ensure a productive research effort; 2) Monitor the progress of each of the Research and Pilot projects and their interactions with the scientific cores; 3) Provide an efficient, centralized unit for the fiscal and administrative operation of SHIMR Center activities; 4) Provide infrastructure support for Stanford SHIMR Center investigators to develop collaborative studies with other members of the consortium and research groups.
In summary, we wish to analyze a variety of different disease/vaccine models in order to define common and unique characteristics of responder and non-responder individuals. We will develop new informative tools and assays that should be illuminating both to specific questions regarding these study groups and will also be of benefit generally with respect to
|Qi, Qian; Liu, Yi; Cheng, Yong et al. (2014) Diversity and clonal selection in the human T-cell repertoire. Proc Natl Acad Sci U S A 111:13139-44
|Chang, Serena; Kohrt, Holbrook; Maecker, Holden T (2014) Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother 63:713-9
|Qi, Qian; Zhang, David W; Weyand, Cornelia M et al. (2014) Mechanisms shaping the naïve T cell repertoire in the elderly - thymic involution or peripheral homeostatic proliferation? Exp Gerontol 54:71-4
|Shekhar, Karthik; Brodin, Petter; Davis, Mark M et al. (2014) Automatic Classification of Cellular Expression by Nonlinear Stochastic Embedding (ACCENSE). Proc Natl Acad Sci U S A 111:202-7
|Jackson, Katherine J L; Liu, Yi; Roskin, Krishna M et al. (2014) Human responses to influenza vaccination show seroconversion signatures and convergent antibody rearrangements. Cell Host Microbe 16:105-14
|Lu, Yuan; Welsh, John P; Swartz, James R (2014) Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. Proc Natl Acad Sci U S A 111:125-30
|Wang, Chen; Liu, Yi; Xu, Lan T et al. (2014) Effects of aging, cytomegalovirus infection, and EBV infection on human B cell repertoires. J Immunol 192:603-11
|O'Gorman, William E; Huang, Huang; Wei, Yu-Ling et al. (2014) The Split Virus Influenza Vaccine rapidly activates immune cells through Fc? receptors. Vaccine 32:5989-97
|Rosenberg-Hasson, Yael; Hansmann, Leo; Liedtke, Michaela et al. (2014) Effects of serum and plasma matrices on multiplex immunoassays. Immunol Res 58:224-33
|Kay, Alexander W; Fukuyama, Julia; Aziz, Natali et al. (2014) Enhanced natural killer-cell and T-cell responses to influenza A virus during pregnancy. Proc Natl Acad Sci U S A 111:14506-11
Showing the most recent 10 out of 40 publications


text:
Comments: Great brace! I am a junior player, and before wearing these braces I would roll, twist, and sprain
my ankles all the time. Now that I wear these braces on both ankles I feel a extremely high level of support when on the
court. I play everyday about three hours a day, so my ankles are always sore after training, but these cut my pain down.
The best part about the ASO brace is that it is very lightweight, but still provides great support. The only downside is that it
is a little hard to tie your shoes, so just get a pair of longer laces.
Comments: After spraining my ankle, I tried almost every brand of ankle brace available. I love the ASO
They provide great support, but aren't so rigid that they rub or bind anywhere on your lower legs, ankles or feet. Even
though my
ankle is healed, I still wear them just so I can confidently move and make quick turns without fear of further injury. I wear
same size shoe, but on some pairs, don't use the top set of eyelets.
Comments: I did a lot of research before I bought this ankle brace, and the work was well worth it. This brace
excellent. I rolled my ankle playing tennis and fractured a bone in my foot. When I was cleared to play, my ankle was still a
sore and I was terrified of rolling it again. With this on, I feel 100% confident that I won't roll it again (I've been using it now
about 3 months). It did take me 2 or 3 times out to get used to the feel of it, but after that I didn't even notice that I had it
on and
it doesn't hamper my mobility to move around the court. It does make my foot hot, but it's well worth it because of the
security it
provides. For those of you struggling to decide on the size, I am a size 9 and have a narrow foot. I ordered a small and it's
perfect. Some people said they had to go up a shoe size, I didn't.
Comments: The ASO Ankle Brace provided me with a lot of support. But unfortunately, the ankle brace can
your ankle super hot since there isn't much ventilation.
Comments: Great brace. I began using them after rolling my ankle and tearing ligaments and now use them
both ankles in a preventive capacity. Comfortable and they lead to confidence. Regarding changing shoe size - in my case
been able to stay with the same size as before.
Comments: I suffered an avulsion fracture of the fifth metatarsal. My doctor approved the ASO brace and I
been wearing it regularly. I have regained full mobility and won't play or practice without it. One thing I have noticed is that
purchase my shoes 1/2 to 1 size larger to accommodate the brace. Great confidence builder.
Comments: I am using it mostly to enhance my performance on court. I found it improves my acceleration,
stopping and changing directions. Next morning, even after toughest match I can walk downstairs normally, not on my
waking up my whole family with moans and groans.
Give it a try, as it gives unfair advantage on tennis court.
Comments: Took some time to get used to having them on but these are great. I never feel like they are
my movement but I always feel much more secure.
Comments: These are the most wonderful ankle braces I have ever worn. I play basketball and I
recently injured my ankle. It has partly healed but this ankle brace optimizes my performance on the court. It is
thin and can fit inside my shoe without stretching it out yet provides more durability and support than most thick
ankle braces. I definitely recommend the ASO ankle brace to any athletes out there.
Comments: I really like the ASO Ankle Brace. I'm getting over a strained deltoid ligament and stress
fracture in my right ankle. I've tried several different braces and the ASO Ankle Brace is the most comfortable.
The seams don't dig into my skin while I play tennis and give me a lot of support. Plus the material is a lot more
light weight. I'm ordering two more so I can rotate them and also have one for my left ankle as a precaution.
Comments: These are GREAT! I have been using these for the past 6 years! As far as shoes go, I
just want a pair
that are comfortable on the BOTTOM of my feet! With these braces I feel VERY SECURE and comfortable!
Comments: I ordered a pair of ASO Ankle Braces. I had ankle injury (with 3 to 9 weeks of not
playing). I play
basketball. I use them and I am not afraid to take rebounds now! It's the best ankle brace I have ever had. It
keeps the foot free
but not with the inversion movement. Recommended for all the person who have had an ankle injury.
Comments: These ankle braces really do work. I previously broke my ankle and these have really helped to
keep me
going and help me from twisting an ankle again.
Comments: Great support and stability. I tore up my ankle pretty badly twice, and now am using it to prevent it
from happening again. I feel confident in change of directions, and it's also very comfortable. It doesn't restrict my lateral
movement very much, in fact I find it gives more support on sprints. Above all, it's very comfortable.


text:
B2 using the real or original meaning of a word or phrase名副其实地,按字面含义来讲
They were responsible for literally millions of deaths. 他们的确对数百万人的死负有责任。
› informal used to emphasize what you are saying(用于强调所说的话)确实,真正地
› informal simply or just只是,仅仅,就
More examples
- He literally flew across the room.
- I literally had a heart attack when I heard the news.
- They were literally over the moon at the news.
- She was literally tearing her hair out with grief.
- He was literally millions of miles ahead of the other runners.


text:
If you are also plantng bulbs this year, while wearing very strong heavy gloves, put some of the cuttings around the bulbs - it keeps the squirrels off the bulbs.
Pyracantha really does not mind when you cut it, I tend to do it around now - plus the odd bit that I feel is getting away whenever I feel like it. This year there are very many berries, so the birds will be happy. Much of the pruning is of new shoots which have sprouted out of the hedge, so you don't lose as many berries as you might think. I prefer not to do it in the spring as a) there are far too many other things want doing and b) several birds build their nests in that hedge, so best left well alone. Put on as much armour plating as you can, it really is such a ferocious thing - bit so beautiful in full flower or berry - and so many insects and birds love it.
Who told you thst isn't spectacular? They fibbed!!
Well, that is interesting - I was joking, had no idea - but I suspect that is a good idea on their part.
Yes it will work, but indeed you will have quite a long wait - you will get grassy type leaves next year, these growing bigger until the plant is mature enough to flower - by which time it will have grown a 'bulb' big enough for it to use. I have popped seeds in the ground (of ordinary lilies not the tree types) and been pleasantly surprised a few years later when a new lily comes into flower. I just put them in by the fence and forgot about them, it seemed to work then! To do it properly with slowly enlarging pots will take years - but oh, how satisfying. It is of course what the breeders of new lilies must do - maybe you could grow a new hybrid and make a fortune - or maybe not!
I keep mine in the cool greenhouse overwinter. Don't forget that you can make lots of new plants with the big leaves, each leaf cut across into slices, and planted in gritty compost (the right way up, I lost alot doing them the wrong way!), will give you new plants to either keep or give away, all for free.
It's so lovely to see you using the spelling of Rosemarie for the beautifully scented herb, usually spelled Rosemary - I like it as it is my name and the way I spell it. I agree with you regarding cleaning agents, the use of herbs not only smells good, but many of them are as you say, disinfectant and antimicrobial. Rosemarie is one as is mint, especially the deeper green ones such as black perppermint. Of course pine needles are good too, which is why so many 'disinfectants' are artificially perfumed with it. A long soak of the needles, strain the fluid and use for floors, surfaces etc., and bathrooms benefit from it too. If people do not wish to use fresh herbs, or do not have access to them, maybe at least use decently sourced, earth friendly cleaning items without all the articial stuff in them - saving some of what is left of the planet.
I haven't got a picture at present, but my ginko is the most glorious shade of deep butter yellow, as it is every year at ths time. Clear colour, no spotting, just stunningly good.
If this is potted, or could be, then a root restricting bag might be an answer? I don't know if you can use them in the ground, I imagine the roots would just escape and you'd have a big tree anyway. Why not get a tree suitable for the site? If you must have it and don't already, then a good clay pot and the aforementioned root restriction bag might do the trick.
Figrat, no I don't mind you asking at all - tempis has fugited qute a while now, and some of the bones and joints just don't do as they used to do - they call it arthritis, I call it a b****** nuisance, but there we are - it still all works after a fashion, just a rather odd fashion, but so far we still get compost - and I still garden!
Daniel, thanks for your post - it's a problem only when we don't know quite how to deal with it. There are always one or two voices who speak out of turn - there are lots of very serious forums (fora??) on the web - maybe they would enjoy those more. Love the chat here, and that so many of the posters came from the old BBC site which closed, and were accepted and joined in with the people already here. We all need information and help, and sometimes it is nice to be able to offer some as well -- or even just to moan about squirrels and pigeons.


text:
Philip Varney eBooks
eBooks found: 1
Ghost Towns of the Mountain West
Philip Varney
Voyageur Press, July 2010
Format: ePub
eBookMall is a recognized leader in eBooks. eBook downloads in PDF and ePub formats.
Choose from 600,000+ eBooks and get a Free eBook download now!
Copyright © 2000-2017 eBookMall.com This website is subject to copyright laws and may be covered by other intellectual property.
All rights reserved.


text:
Cet Maison 2 chambres louer Chetwynd est maintenant disponible.
Wentworth Townhomes
2 & 3 bedroom townhomes with small fenced yard. Perfect for families and are pet friendly (pets must meet approval). Homes are 2 levels with main living area & laundry area (washer, dryer, fridge & stove included)on main level. Bedrooms and bath on upper floor. Suites have been updated with modern paint, flooring, appliances & light fixtures


text:
Nokia Lumia 1020 Review
Latest Camera Reviews
Mac users, we're pleased to announce Macphun's all-in-one photo editor Luminar is now available for just $69£52 with special Valentine Day bonuses (two eBooks, Vivid Wonderland preset pack, & Creative Sky Overlay pack) included for free until February 19. Use coupon code "PHOTOBLOG" to save another $10 on Luminar.
We rated Luminar as "Highly Recommended". Visit the Luminar web site to try it for free.
Entry Tags
review, 1080p, test, movie, video, camera, smartphone, JPEG, movies, image stabilisation, phone, 3x zoom, 26mm, videos, OIS, full HD, 6x zoom, Nokia, Nokia Lumia 1020, 41 megapixel, Lumia 1020, f2.2


text:
Wilson's blurry because he's traveling at a very high rate of speed.
The last time Washington Capitals rookie Tom Wilson played the New York Islanders, the big, bruising forward showed off his soft hands, scoring his first NHL point (from his tush) and his first NHL goal.
Saturday night, Wilson showed off another part of his game to the Islanders: his physicality. Ten minutes into the first period, the 6'4" Wilson leveled the 6'2", 215-pound Colin McDonald, smearing him into the boards.
Russian Machine Never Breaks is not associated with the Washington Capitals; Monumental Sports, the NHL, or its properties. Not even a little bit.
All original content on russianmachineneverbreaks.com is licensed under Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)– unless otherwise stated or superseded by another license. You are free to share, copy, and remix this content so long as it is attributed, done for noncommercial purposes, and done so under a license similar to this one.


text:
Reminiscent of Pretty Woman with the escargot?
> [Original Message]
> From: cathy carpenter <email@example.com>
> To: <firstname.lastname@example.com>
> Date: 1/25/2003 1:10:35 PM
> Subject: Re: [CHAT] was fat birdies-now calamari
> I, too, love calamari, but I ordered it one time in an Italian
> restaurant in D.C. and instead of the sliced squid in marinara I
> expected, got a huge, stuffed individual. It tasted great, but while I
> attempted to slice it, it slid right into my lap! My family still
> chuckles about that.
> Cathy
> ---------------------------------------------------------------------
> To sign-off this list, send email to email@example.com with the
> message text UNSUBSCRIBE GARDENCHAT
To sign-off this list, send email to firstname.lastname@example.com with the
message text UNSUBSCRIBE GARDENCHAT


text: "Join Or Die. That's Your Pitch?"Anna Holmes11/10/09 2:30pmFiled to: ClipsRoger sterlingMad MenRetirement28EditPromoteShare to KinjaToggle Conversation toolsGo to permalink "Retiring Makes You Feel 10 Years Younger". Don't be fooled: this news story is nothing more than an excuse for us to post this video of Roger Sterling one-liners. MM withdrawal can do that to a girl. [Telegraph, NYMag] Reply28 repliesLeave a reply


text: The number of new jobs created in the City of London (the financial and banking district of London) increased slightly in July compared with the previous month, but was still well down on a year ago, according to new research Monday. A study by recruitment firm "Astbury Marsden" showed there were almost 3, 000 new jobs in the City last month, a 3 percent increase on June but 39 percent fewer than the same time in 2011. The report said the jobs market in the sector had now stabilised after a slump, although the appetite to hire new staff was described as "pretty thin." Mark Cameron, chief operating officer at Astbury Marsden, said "Banks are hiring new staff, although this is more often than not to replace departing staff, as opposed to reacting to an anticipated growth in investment banking revenue. "With the uncertainty generated by the on-going rumblings from the Spanish banking sector and the seemingly ever-present eurozone problems, we're not expecting things to improve dramatically this year. Still, in this context, a stabilisation in new jobs is a positive." The banking sector in the US and the UK faced the severe crisis of the credit crunch in 2009. In addition, Europe was now facing the eurozone mounting turbulence that has cast a shadow on the prospects of recovery in the EU, analysts said.


text:
Exosomes from IL-1β stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes
- Tomohiro Kato†1Email author,
- Shigeru Miyaki†1, 2,
- Hiroyuki Ishitobi2,
- Yoshihiro Nakamura1,
- Tomoyuki Nakasa1,
- Martin K Lotz3 and
- Mitsuo Ochi1
DOI: 10.1186/ar4679
© Kato et al.; licensee BioMed Central Ltd. 2014
Received: 28 February 2014
Accepted: 21 July 2014
Published: 4 August 2014
Osteoarthritis (OA) is a whole joint disease, and characterized by progressive degradation of articular cartilage, synovial hyperplasia, bone remodeling and angiogenesis in various joint tissues. Exosomes are a type of microvesicles (MVs) that may play a role in tissue-tissue and cell-cell communication in homeostasis and diseases. We hypothesized that exosomes function in a novel regulatory network that contributes to OA pathogenesis and examined the function of exosomes in communication among joint tissue cells.
Human synovial fibroblasts (SFB) and articular chondrocytes were obtained from normal knee joints. Exosomes isolated from conditioned medium of SFB were analyzed for size, numbers, markers and function. Normal articular chondrocytes were treated with exosomes from SFB, and Interleukin-1β (IL-1β) stimulated SFB. OA-related genes expression was quantified using real-time PCR. To analyze exosome effects on cartilage tissue, we performed glycosaminoglycan release assay. Angiogenic activity of these exosomes was tested in migration and tube formation assays. Cytokines and miRNAs in exosomes were analyzed by Bio-Plex multiplex assay and NanoString analysis.
Exosomes from IL-1β stimulated SFB significantly up-regulated MMP-13 and ADAMTS-5 expression in articular chondrocytes, and down-regulated COL2A1 and ACAN compared with SFB derived exosomes. Migration and tube formation activity were significantly higher in human umbilical vein endothelial cells (HUVECs) treated with the exosomes from IL-1β stimulated SFB, which also induced significantly more proteoglycan release from cartilage explants. Inflammatory cytokines, IL-6, MMP-3 and VEGF in exosomes were only detectable at low level. IL-1β, TNFα MMP-9 and MMP-13 were not detectable in exosomes. NanoString analysis showed that levels of 50 miRNAs were differentially expressed in exosomes from IL-1β stimulated SFB compared to non-stimulated SFB.
Exosomes from IL-1β stimulated SFB induce OA-like changes both in vitro and in ex vivo models. Exosomes represent a novel mechanism by which pathogenic signals are communicated among different cell types in OA-affected joints.
Osteoarthritis (OA) is a highly prevalent disease in the middle-aged and elderly population worldwide. Pathogenesis has not been elucidated completely, and disease-modifying treatment and prevention are presently not available. OA risk factors include aging, acute or chronic mechanical stress, joint trauma, and metabolic disorders [1, 2]. These factors impair the homeostatic balance between cartilage extracellular matrix (ECM) degradation and repair. OA is a whole-joint disease and involves all joint tissues including cartilage, subchondral bone, menisci, ligaments and muscles. Joint inflammation at varying intensity is also present and contributes to the chronic joint tissue remodeling process and to pain, the main subjective symptom in OA patients [2–6]. The homeostatic balance of all joint tissues is regulated by intracellular molecules such as kinase cascades, autophagy, and transcription factors, epigenetic mechanisms, including miRNAs and by extracellular stimuli including cytokines, hormones and mechanical stress [7, 8].
Synovial inflammation and angiogenesis are important contributors to OA pathogenesis [3, 9–11]. Synovitis has been demonstrated to correlate with OA symptom severity, and hormonal factors such as cytokines, and chemokines are important for crosstalk among joint tissues [3, 12]. These mediators play a role in the development of inflammation and induce catabolic changes in joint tissues [3, 11, 13, 14]. Increased angiogenesis is also observed in OA-affected ligaments, menisci and subchondral bone [5, 6, 15].
Most nucleated cells release microvesicles (MVs), which range from 30 to 1,000 nm in diameter, and can be found in body fluids such as blood, urine, breast milk, and saliva [16–18]. MVs are also present in rheumatoid arthritis (RA) synovial fluids and can originate from granulocytes, monocytes, and other immune cells. These MVs modulate the release of chemokines and cytokines in synovial fibroblasts (SFB) [19–21]. The MVs derived from OA chondrocytes display annexins II, V, and VI, which play an important role in pathological mineral formation in OA [22–24]. Exosomes are one type of MV of endocytic origin released to the extracellular environment. These small particles, of about 30 to 200 nm, are derived from the fusion of multivesicular bodies to plasma membranes, and are morphologically distinct from larger secreted MVs [18, 25]. Exosomes can contain mRNA, microRNA and protein [26] and function in cell-to-cell communication as carriers of genetic information, and are associated with the pathogenesis of various diseases [19, 27]. Although release of MVs from SFBs has been reported [21, 28, 29], the effects of exosomes from OA synovial tissues on articular chondrocytes remain unknown. We hypothesized that exosomes function in a novel regulatory network that contributes to OA and elucidate in the present study the in vitro and ex vivo production, and the function of exosomes in the interaction between SFB and articular chondrocytes.
Materials and methods
Human tissues and cell culture
Studies were approved human subjects/ethics protocols by Scripps Research Institute Human Subjects Institutional Review Boards. Normal human knee synovial fibroblasts and chondrocytes were isolated from autopsy donors as leftover de-identified material and with no interactions with subjects, and therefore with no informed consent required. Human SFB and articular chondrocytes were cultured as described previously [30] in DMEM containing 10% FBS, and 1% penicillin/streptomycin at 37°C in a humidified atmosphere of 5% CO2. Experiments with SFB were carried out at passages 4 to 6. Experiments with normal human articular chondrocytes were carried out at passage 1.
Preparation of conditioned medium and isolation of exosomes
Human SFB were plated at 2 × 105 per well into 6-well plates with DMEM containing 10% FBS and 1% antibiotics at 37°C under 5% CO2. After reaching confluence, the cells were treated with DMEM containing recombinant human IL-1β (1 ng/mL; Pepro Tech NJ, USA), and incubated for 24 h. The SFB were washed three times with DMEM, and the medium was switched to fresh DMEM containing 10% FBS (2 mL). After incubation for 24 h, the SFB conditioned media (2 mL/well) were collected and cells were washed with PBS, and total RNA was isolated with TRIzol reagent (Life Technologies). The most widely accepted method for exosome isolation is ultracentrifugation. Recently, however, several alternate methods that are more standardized and efficient have been developed. In the present study, we used conventional ultracentrifugation and ExoQuick™ reagent kit (System Biosciences CA, USA) for isolation of exosomes [31–33], because these methods did not show a detectable difference in the type of exosomes obtained when applied to supernatants from cultured cells. The SFB-conditioned media (2 mL/well) were centrifuged for 15 minutes at 2380 × g to remove debris, and then further ultracentrifuged for 70 minutes at 110,000 × g (Optima TL ultracentrifuge, Beckman Coulter, Brea, CA, USA). The supernatants were collected as exosome-depleted conditioned media (CM-exo). The purified exosomes were resuspended in DMEM for direct use in subsequent experiments.
Size distribution analysis by tunable resistive pulse sensor (TRPS)
Exosomes isolated by ultracentrifugation were resuspended in 100 mM KCl and 40 mM HEPES. The measurements of particle size and number were performed on an Izon qNano system by TRPS technology (Izon Science, Ltd Christchurch, New Zealand) as previously reported [34, 35].
Immunoblotting for exosome markers
Exosomes and proteins isolated from cells were suspended in sample buffer solution (not containing 2-mercaptoethanol; Wako, Japan). Proteins were separated on Mini-PROTEAN TGX gels (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to a polyvinylidene fluoride (PVDF) membrane by Trans-Blot Turbo™ transfer system (Bio-Rad Laboratories). Mouse anti-human CD9 (1:200, BD Biosciences), anti-CD81 (1:200, Santa Cruz Biotechnology, Inc), and anti-flotillin-1 (1:500, BD Biosciences) were used as the primary antibodies. Goat anti-mouse IgG-HRP (Santa Cruz, sc-2005) was used as the secondary antibody. Chemiluminescence signal was detected with Immno-enhancer (WAKO, Japan) using the ImageQuant LAS-4000 mini luminescent image analyzer (FUJIFILM, Japan).
Cytokine levels in exosomes and conditioned medium from SFB
The measurements of cytokines (IL-1β, IL-6, TNFα, VEGF) and MMPs (MMP-3, -9, -13) within exosomes, CM-exo and conditioned media (CM) from SFB were performed by Bio-Plex suspension assay system (Bio-Rad Laboratories) as per the manufacturer's protocol. Exosomes and CM-exo were isolated from conditioned medium by ultracentrifugation and the ExoQuick™ reagent kit.
Treatment of normal human articular chondrocytes with SFB-derived exosomes
Human articular chondrocytes were seeded at 5 × 104/ well into 24-well plates in DMEM containing 10% FBS, and cultured for 24 h. After the cells became confluent, exosome suspensions in DMEM and CM-exo were added. After incubation for 24 h, total RNA was extracted from chondrocytes using TRIzol reagent (Life Technologies).
Quantitative real-time PCR
Complementary DNA (cDNA) was synthesized using 500 ng of total RNA with Superscript VILO kit (Invitrogen) according to the manufacturer's protocol. Real-time PCR assay was performed using TaqMan Gene Expression Assays probes (Life Technologies) for COL2A1 (Hs01064869_m1), ACAN (Hs00202971_m1), MMP-13 (Hs00233992_m1), ADAMTS-5 (Hs00199841_m1), IL-6 (Hs00985639_m1), MMP-3 (Hs00233962_m1), IL-1β (Hs00174097_m1), TNFα (Hs00174128_m1) and GAPDH (Hs02758991_m1). The expression levels for each gene were assessed relative to the expression of GAPDH. The ΔΔCt method was used for analysis of real-time PCR data.
Proteoglycan release assay
Femoral heads were harvested from 4-week-old wild-type mice and incubated at 37°C for 72 h in 48-well plates. Each well contained 500 μl of DMEM with 10% FBS and 1% penicillin/streptomycin. The cartilage samples were washed three times, and cultured at 37°C for an additional 72 h in 500 μl of serum-free DMEM containing exosomes isolated by ExoQuick™ from SFB with IL-1β or without IL-1β. The assay was performed in at least three independent experiments with duplicate wells using SFB-derived exosomes. The concentration of the released glycosaminoglycan in the conditioned medium from cartilage was measured using the Blyscan Glycosaminoglycan assay kit (Biocolor UK) as per the manufacturer's protocol.
Endothelial cell migration assay
Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza and cultured with endothelial basal medium-2 (EBM-2) (Lonza Basel, Switzerland). The migration of HUVECs was assessed using a Transwell Boyden Chamber (8-μm pore size) (BD Bioscience). HUVECs suspended in 0.5 mL of serum-free DMEM containing exosomes were added at 2 × 104 cells/well to the 24-well upper chamber, and 0.75 mL of DMEM was added to the bottom well. After incubation for 4 h, the lower side of the filter was washed with PBS and fixed with 4% paraformaldehyde. The migrated cells were quantified as the number of cell nuclei stained with 4',6-diamidino-2-phenylindole (DAPI).
Tube formation assay
HUVECs were pre-cultured overnight in DMEM with 0.25% FBS and then reseeded at a density of 2.5 × 104 cells/well in 48-well plates pre-coated with Matrigel Matrix (BD Biosciences). HUVECs were treated with DMEM with 0.25% FBS containing, vascular endothelial growth factor (VEGF) (50 ng/mL as positive control), IL-1β (1 ng/mL) and exosomes for 4 h. Tube length was measured by means of Image J analysis of digital images.
miRNA analysis in exosomes from SFB-conditioned medium
Exosomes were isolated from 15 mL of conditioned medium from SFB seeded at 1.5 × 106 cells/15 cm plate by ultracentrifugation. Small RNA was purified from exosomes using the mirVana isolation kit (Life Technologies). The small RNA concentration and quality were determined by BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and at least 5 ng RNA were then used as input for the nCounter Human miRNA Expression Assay kit (NanoString Technologies, Seattle, WA, USA). The miRNA expression profiles were analyzed according to manufacturer's instructions.
Statistical analysis
The data were analyzed using the Mann-Whitney U-test and Steel test to analyze statistical differences. Differences were considered statistically significant at a P-value <0.05 (*P <0.05; **P <0.01).
Effect of IL-1β on gene expression in SFB
Exosomes in IL-1β-stimulated SFB-conditioned medium
Exosomes from IL-1β-stimulated SFB induce OA-related gene expression patterns in articular chondrocytes
Proteoglycan release from cartilage explants by exosomes from IL-1β-stimulated SFB
SFB derived exosomes induce angiogenesis
miRNA in exosomes from OA-like SFB
This study focused on exosomes as a new secretion and communication factor in OA. IL-1β is one of the most prominent mediators of cartilage degradation and joint inflammation [11, 13, 37]. Therefore, we used IL-1β to simulate an OA environment in the SFB and their conditioned medium. Recently, extracellular vesicles, including exosomes, have attracted attention as new players in cell-to-cell communication [26, 38–40]. Exosomes may directly stimulate target cells through receptor-mediated interactions or may transfer from the host cell to the recipient cell various bioactive molecules such as proteins, mRNA and miRNA [27]. Exosomes are defined as small vesicles between 30 and 100 nm in diameter [18]. Although they are distinguished from other MVs based on their size, some differences are still unclear. Exosomes are formed intracellularly within multivesicular bodies and they have common surface components, such CD9, CD63, CD81, and flotillin-1 [25, 36]. Although MVs (100 to 1,000 nm in diameter) are larger than exosomes, they are shed from the plasma membrane surface. MVs that are 200 to 700 nm in diameter and are released from lymphocytes and monocytes induce MMPs and cytokine expression by SFB [21]. The exosomes isolated from SFB-conditioned media ranged in size mainly between 60 nm and 120 nm, and expressed exosome markers, including CD9, CD81 and flotillin-1. Furthermore, the release of exosomes was increased by IL-1β stimulation to SFB. Although the mechanisms of exosome release from cells remain incompletely understood, the release of exosomes has been shown to be affected by the pathological environment and culture condition [41–43]. These reports suggest that the OA pathological tissue environment regulates the release of exosomes from joint cells, including SFB. In this regard, exosomes may also reflect the specific disease environment. Therefore, exosomes have potential as diagnostic markers, and also offer insight into new regulatory pathways of pathogenesis.
MMPs, inflammatory cytokines and growth factors cause OA-like changes in joint tissues [10, 11, 13]. IL-1β-stimulated SFB and SFB from osteoarthritic joints can secrete a variety of cytokines and growth factors in vivo and in vitro, including MMPs, IL-6 and VEGF, which is a major factor in inflammation and angiogenesis [11, 13, 37]. Indeed, we showed that exosomes from IL-1β stimulated SFB increased the expression of MMP-3, IL-6, and VEGF. MMP-3, IL-6 and VEGF were present in CM and CM-exo. However, their levels were significantly lower in exosomes than in CM and CM-exo. After ultracentrifugation for isolation of exosomes, the supernatants were collected as CM-exo. Indeed, exosome markers such as CD9 and flotillin-1 were not detected in CM-exo (data not shown). These results indicate that almost IL-6 and VEGF were contained in CM-exo after exosomes isolation by ultracentrifugation method. Although CM-exo induced the OA-like gene expression patterns in articular chondrocytes, exosomes from IL-1β-stimulated SFB also induced the OA-like gene expression patterns, and they stimulated proteoglycan release from cartilage explants. Therefore, the OA-like changes may be not only attributable to cytokines and growth factors, but also to exosomes. The exosomes from IL-1β-stimulated SFB induced expression of OA-related genes, especially in articular chondrocytes. These results suggest that other factors, including miRNA in exosomes, mediate OA-like changes. Furthermore, we showed that IL-1β-stimulated SFB-derived exosomes induced angiogenic activities such as migration and tube formation in HUVEC. Increased angiogenesis occurs in most tissues in osteoarthritic joints, including synovium, menisci, ligaments and subchondral bone and inhibition of angiogenesis has been shown to be effective in models of OA [3, 9–11]. Therefore, exosomes may promote increased angiogenesis in OA joint tissues. Taken together, SBF-derived exosomes may function as a novel regulatory factor that contributes to OA pathogenesis via OA-like signals such as catabolic and angiogenic signals to joint tissues.
miRNA are small noncoding RNA of 1 to 25 nucleotides that repress the translation of and/or cleave mRNA by partially base-pairing with the untranslated regions and/or coding regions of their target transcripts [44]. Therefore, miRNA can silence the expression of multiple genes [44]. Several miRNA, such as miR-140, are associated with OA pathogenesis [7, 45, 46]. Although previous studies have focused on intracellular miRNA, miRNA have recently been identified in several extracellular compartments. Exosomes contain proteins, mRNA and miRNA, and protect miRNA from RNase [26, 36], and miRNA can be transferred between tissues and even between individuals via biological fluids [16, 26]. However, it is reported that the extracellular miRNA in blood plasma and cell culture is independent from exosomes and is bound to Ago2 protein, a part of RNA-induced silencing complex [47, 48]. Thus, we may have identified extracellular miRNA rather than miRNA in exosomes. IL-1β stimulation of SFB increased the amount of released exosomes from SFB. Furthermore, the IL-1β stimulation altered the miRNA expression pattern not only in SFB but also in released exosomes. Although most of the upregulated miRNAs in IL-1β-stimulated SFB were also upregulated in their exosomes, several miRNA were identified only in exosomes. This result suggests a selective mechanism of miRNA release from cells. Indeed, although several studies reported these findings [26, 38–40, 49, 50], this mechanism is not still well-understood. Among upregulated miRNA (>2-fold) in exosomes from IL-1β-stimulated SFB, six miRNA were selectively present in exosomes. MiRNA in condition-specific exosomes may function as a unique set of miRNA. These results suggest that miRNA may not only function as regulatory molecules within cells such as SFB and chondrocytes, but also as components of exosomes contributing to OA pathogenesis by mediating cell-cell and tissue-tissue communication in osteoarthritic joints. Future studies need to determine the amount of exosomes released from non-OA- and OA-derived tissues and from cells, and the amount present in synovial fluid, and to identify the messenger molecules in exosomes that mediate the observed effects on chondrocytes.
The present results show that SFB secrete exosomes, and IL-1β-stimulated SFB release increased the numbers of exosomes compared with non-stimulated SFB. miRNA are selectively presented in the exosomes. Exosomes from IL-1β-stimulated SFB lead to OA-like changes in gene expression patterns in human articular chondrocytes, and cartilage degradation. In addition, these exosomes stimulated angiogenic activity. SFB-derived exosomes may be involved in OA pathogenesis by stimulating gene expression in articular chondrocytes, and promoting angiogenesis. Our observations suggest that not only established signaling molecules, such as cytokines and hormones, but also exosomes including miRNA, as mediators of communication among different joint cells and tissues play an important role in OA pathogenesis as a new regulatory mechanism.
a disintegrin and metalloproteinase with thrombospondin motifs
conditioned media
- CM-exo:
exosome-depleted conditioned media
- Cont:
Dulbecco's modified Eagle's medium
endothelial basal medium-2
extracellular matrix
fetal bovine serum
- Flot:
human umbilical vein endothelial cells
matrix metalloproteinase
normal human synovial fibroblast
osteoarthritis synovial fibroblast
phosphate-buffered saline
synovial fibroblast
tumor necrosis factor
vascular endothelial growth factor.
We thank M Fuka, T Miyata, M Yoshida and T Nishimura for their technical support. We also thank M Miyaki, DVM, PhD for assistance with bioinformatics. This research was supported by MEXT KAKENHI Grant-in-Aid for Scientific Research (A) grant number 21249079 (MO), young scientists (A) grant number 24689057 (SM), Takeda Science Foundation (SM), Japan Rheumatism Foundation (SM) and Setsuro Fujii Memorial, the Osaka Foundation for Promotion of Fundamental Medical Research (SM) and NIH grant AG007996 (ML). A part of this work was carried out at the Analysis Center of Life Science, Natural Science Center for Basic Research and Development, Hiroshima University.
Authors' Affiliations
- Lotz MK, Kraus VB: New developments in osteoarthritis. Posttraumatic osteoarthritis: pathogenesis and pharmacological treatment options. Arthritis Res Ther. 2010, 12: 211-PubMed CentralView ArticlePubMedGoogle Scholar
- Loeser RF, Goldring SR, Scanzello CR, Goldring MB: Osteoarthritis: a disease of the joint as an organ. Arthritis Rheum. 2012, 64: 1697-1707.PubMed CentralView ArticlePubMedGoogle Scholar
- Scanzello CR, Goldring SR: The role of synovitis in osteoarthritis pathogenesis. Bone. 2012, 51: 249-257.PubMed CentralView ArticlePubMedGoogle Scholar
- Hasegawa A, Otsuki S, Pauli C, Miyaki S, Patil S, Steklov N, Kinoshita M, Koziol J, D'Lima DD, Lotz MK: Anterior cruciate ligament changes in the human knee joint in aging and osteoarthritis. Arthritis Rheum. 2012, 64: 696-704.PubMed CentralView ArticlePubMedGoogle Scholar
- Levy YD, Hasegawa A, Patil S, Koziol JA, Lotz MK, D'Lima DD: Histopathological changes in the human posterior cruciate ligament during aging and osteoarthritis: correlations with anterior cruciate ligament and cartilage changes. Ann Rheum Dis. 2013, 72: 271-277.PubMed CentralView ArticlePubMedGoogle Scholar
- Pauli C, Grogan SP, Patil S, Otsuki S, Hasegawa A, Koziol J, Lotz MK, D'Lima DD: Macroscopic and histopathologic analysis of human knee menisci in aging and osteoarthritis. Osteoarthritis Cartilage. 2011, 19: 1132-1141.PubMed CentralView ArticlePubMedGoogle Scholar
- Miyaki S, Asahara H: Macro view of microRNA function in osteoarthritis. Nat Rev Rheumatol. 2012, 8: 543-552.PubMed CentralView ArticlePubMedGoogle Scholar
- Lotz MK, Carames B: Autophagy and cartilage homeostasis mechanisms in joint health, aging and OA. Nat Rev Rheumatol. 2011, 7: 579-587.PubMed CentralPubMedGoogle Scholar
- Lambert C, Mathy-Hartert M, Dubuc JE, Montell E, Verges J, Munaut C, Noel A, Henrotin Y: Characterization of synovial angiogenesis in osteoarthritis patients and its modulation by chondroitin sulfate. Arthritis Res Ther. 2012, 14: R58-PubMed CentralView ArticlePubMedGoogle Scholar
- Sellam J, Berenbaum F: The role of synovitis in pathophysiology and clinical symptoms of osteoarthritis. Nat Rev Rheumatol. 2010, 6: 625-635.View ArticlePubMedGoogle Scholar
- Goldring MB, Otero M: Inflammation in osteoarthritis. Curr Opin Rheumatol. 2011, 23: 471-478.PubMed CentralView ArticlePubMedGoogle Scholar
- Goldring MB, Marcu KB: Cartilage homeostasis in health and rheumatic diseases. Arthritis Res Ther. 2009, 11: 224-PubMed CentralView ArticlePubMedGoogle Scholar
- Goldring MB, Goldring SR: Osteoarthritis. J Cell Physiol. 2007, 213: 626-634.View ArticlePubMedGoogle Scholar
- Lotz M, Loeser RF: Effects of aging on articular cartilage homeostasis. Bone. 2012, 51: 241-248.PubMed CentralView ArticlePubMedGoogle Scholar
- Bonnet CS, Walsh DA: Osteoarthritis, angiogenesis and inflammation. Rheumatology (Oxford). 2005, 44: 7-16.View ArticleGoogle Scholar
- Kosaka N, Izumi H, Sekine K, Ochiya T: microRNA as a new immune-regulatory agent in breast milk. Silence. 2010, 1: 7-PubMed CentralView ArticlePubMedGoogle Scholar
- Michael A, Bajracharya SD, Yuen PS, Zhou H, Star RA, Illei GG, Alevizos I: Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis. 2010, 16: 34-38.PubMed CentralView ArticlePubMedGoogle Scholar
- Thery C, Ostrowski M, Segura E: Membrane vesicles as conveyors of immune responses. Nat Rev Immunol. 2009, 9: 581-593.View ArticlePubMedGoogle Scholar
- Anderson HC, Mulhall D, Garimella R: Role of extracellular membrane vesicles in the pathogenesis of various diseases, including cancer, renal diseases, atherosclerosis, and arthritis. Lab Invest. 2010, 90: 1549-1557.View ArticlePubMedGoogle Scholar
- Berckmans RJ, Nieuwland R, Kraan MC, Schaap MC, Pots D, Smeets TJ, Sturk A, Tak PP: Synovial microparticles from arthritic patients modulate chemokine and cytokine release by synoviocytes. Arthritis Res Ther. 2005, 7: R536-R544.PubMed CentralView ArticlePubMedGoogle Scholar
- Distler JH, Jungel A, Huber LC, Seemayer CA, Reich CF, Gay RE, Michel BA, Fontana A, Gay S, Pisetsky DS, Distler O: The induction of matrix metalloproteinase and cytokine expression in synovial fibroblasts stimulated with immune cell microparticles. Proc Natl Acad Sci U S A. 2005, 102: 2892-2897.PubMed CentralView ArticlePubMedGoogle Scholar
- Ali SY, Griffiths S: Formation of calcium phosphate crystals in normal and osteoarthritic cartilage. Ann Rheum Dis. 1983, 42: 45-48.PubMed CentralView ArticlePubMedGoogle Scholar
- Kirsch T, Swoboda B, Nah H: Activation of annexin II and V expression, terminal differentiation, mineralization and apoptosis in human osteoarthritic cartilage. Osteoarthritis Cartilage. 2000, 8: 294-302.View ArticlePubMedGoogle Scholar
- Kirsch T: Annexins - their role in cartilage mineralization. Front Biosci. 2005, 10: 576-581.View ArticlePubMedGoogle Scholar
- Vlassov AV, Magdaleno S, Setterquist R, Conrad R: Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta. 1820, 2012: 940-948.Google Scholar
- Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007, 9: 654-659.View ArticlePubMedGoogle Scholar
- Camussi G, Deregibus MC, Bruno S, Cantaluppi V, Biancone L: Exosomes/microvesicles as a mechanism of cell-to-cell communication. Kidney Int. 2010, 78: 838-848.View ArticlePubMedGoogle Scholar
- Skriner K, Adolph K, Jungblut PR, Burmester GR: Association of citrullinated proteins with synovial exosomes. Arthritis Rheum. 2006, 54: 3809-3814.View ArticlePubMedGoogle Scholar
- Zhang HG, Liu C, Su K, Yu S, Zhang L, Zhang S, Wang J, Cao X, Grizzle W, Kimberly RP: A membrane form of TNF-alpha presented by exosomes delays T cell activation-induced cell death. J Immunol. 2006, 176: 7385-7393.View ArticlePubMedGoogle Scholar
- Maier R, Ganu V, Lotz M: Interleukin-11, an inducible cytokine in human articular chondrocytes and synoviocytes, stimulates the production of the tissue inhibitor of metalloproteinases. J Biol Chem. 1993, 268: 21527-21532.PubMedGoogle Scholar
- Rekker K, Saare M, Roost AM, Kubo AL, Zarovni N, Chiesi A, Salumets A, Peters M: Comparison of serum exosome isolation methods for microRNA profiling. Clin Biochem. 2014, 47: 135-138.View ArticlePubMedGoogle Scholar
- Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D: Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for Bovine oocyte developmental competence. PLoS One. 2013, 8: e78505-PubMed CentralView ArticlePubMedGoogle Scholar
- Yamada T, Inoshima Y, Matsuda T, Ishiguro N: Comparison of methods for isolating exosomes from bovine milk. J Vet Med Sci. 2012, 74: 1523-1525.View ArticlePubMedGoogle Scholar
- Yang L, Broom MF, Tucker IG: Characterization of a nanoparticulate drug delivery system using scanning ion occlusion sensing. Pharm Res. 2012, 29: 2578-2586.View ArticlePubMedGoogle Scholar
- de Vrij J, Maas SL, van Nispen M, Sena-Esteves M, Limpens RW, Koster AJ, Leenstra S, Lamfers ML, Broekman ML: Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing. Nanomedicine (Lond). 2013, 8: 1443-1458.View ArticleGoogle Scholar
- Kharaziha P, Ceder S, Li Q, Panaretakis T: Tumor cell-derived exosomes: a message in a bottle. Biochim Biophys Acta. 1826, 2012: 103-111.Google Scholar
- Zwerina J, Redlich K, Polzer K, Joosten L, Kronke G, Distler J, Hess A, Pundt N, Pap T, Hoffmann O, Gasser J, Scheinecker C, Smolen JS, van den Berg W, Schett G: TNF-induced structural joint damage is mediated by IL-1. Proc Natl Acad Sci U S A. 2007, 104: 11742-11747.PubMed CentralView ArticlePubMedGoogle Scholar
- Hergenreider E, Heydt S, Treguer K, Boettger T, Horrevoets AJ, Zeiher AM, Scheffer MP, Frangakis AS, Yin X, Mayr M, Braun T, Urbich C, Boon RA, Dimmeler S: Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs. Nat Cell Biol. 2012, 14: 249-256.View ArticlePubMedGoogle Scholar
- Zhang Y, Liu D, Chen X, Li J, Li L, Bian Z, Sun F, Lu J, Yin Y, Cai X, Sun Q, Wang K, Ba Y, Wang Q, Wang D, Yang J, Liu P, Xu T, Yan Q, Zhang J, Zen K, Zhang CY: Secreted monocytic miR-150 enhances targeted endothelial cell migration. Mol Cell. 2010, 39: 133-144.View ArticlePubMedGoogle Scholar
- Kosaka N, Iguchi H, Yoshioka Y, Takeshita F, Matsuki Y, Ochiya T: Secretory mechanisms and intercellular transfer of microRNAs in living cells. J Biol Chem. 2010, 285: 17442-17452.PubMed CentralView ArticlePubMedGoogle Scholar
- Lehmann BD, Paine MS, Brooks AM, McCubrey JA, Renegar RH, Wang R, Terrian DM: Senescence-associated exosome release from human prostate cancer cells. Cancer Res. 2008, 68: 7864-7871.View ArticlePubMedGoogle Scholar
- Sahoo S, Losordo DW: Exosomes and cardiac repair after myocardial infarction. Circ Res. 2014, 114: 333-344.View ArticlePubMedGoogle Scholar
- King HW, Michael MZ, Gleadle JM: Hypoxic enhancement of exosome release by breast cancer cells. BMC Cancer. 2012, 12: 421-PubMed CentralView ArticlePubMedGoogle Scholar
- Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004, 116: 281-297.View ArticlePubMedGoogle Scholar
- Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz MK, Ueno-Kudo H, Asahara H: MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes Dev. 2010, 24: 1173-1185.PubMed CentralView ArticlePubMedGoogle Scholar
- Miyaki S, Nakasa T, Otsuki S, Grogan SP, Higashiyama R, Inoue A, Kato Y, Sato T, Lotz MK, Asahara H: MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates interleukin-1 responses. Arthritis Rheum. 2009, 60: 2723-2730.PubMed CentralView ArticlePubMedGoogle Scholar
- Turchinovich A, Weiz L, Langheinz A, Burwinkel B: Characterization of extracellular circulating microRNA. Nucleic Acids Res. 2011, 39: 7223-7233.PubMed CentralView ArticlePubMedGoogle Scholar
- Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, Mitchell PS, Bennett CF, Pogosova-Agadjanyan EL, Stirewalt DL, Tait JF, Tewari M: Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A. 2011, 108: 5003-5008.PubMed CentralView ArticlePubMedGoogle Scholar
- Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T: Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line. PLoS One. 2010, 5: e13247-PubMed CentralView ArticlePubMedGoogle Scholar
- Pigati L, Yaddanapudi SC, Iyengar R, Kim DJ, Hearn SA, Danforth D, Hastings ML, Duelli DM: Selective release of microRNA species from normal and malignant mammary epithelial cells. PLoS One. 2010, 5: e13515-PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


text:
Jasper Historic High Country
Nicknamed "The First Mountain City," Jasper is located 50 miles north of Atlanta, Georgia. It is also known as the Marble Capital of Georgia. Especially unique is the pink marble as seen at the historic Tate House. Several local attractions draw the interest of sightseers annually.
The city's small town atmosphere lends itself to a friendly, mountain experience with good local restaurants, such as the Woodbridge Inn where Chef Hans Rueffert prepares a daily delight of signature dishes. Taste the local wines of Sharp Mountain Vineyards, golf the links at Big Canoe or Bent Tree, and explore the area's Cherokee heritage.
Just a short drive from Jasper is the town of Talking Rock where you will find antique shops, a historic school house museum and artists' displays.
Visitor Information Center
500 Stegall Drive
Jasper, GA 30143
Located on Hwy 515 about an hour north of Atlanta (take I-575 from Atlanta).Visit Website
Phone: 706-692-5600
OfficialVisitor Information
Free Georgia Travel Guide
The state's official Travel Guide is your complete resource to planning a vacation in Georgia. Find trip ideas, attractions, dining, events, activities, accommodation, map, travel resources and much more.
Website: http://www.exploregeorgia.org
Phone: 1-800-VISIT GA (847-4842)
Jasper Travel Guides
Fainting Goat Rack Card
A brief view of what Fainting Goat has to offer!
Website: http://www.faintinggoatvineyardsandwinery.com
Phone: 706-692-9463
Fainting Goat Wedding Brochure
A summary of weddings at Fainting Goat
Website: http://www.faintinggoatvineyardsandwinery.com
Phone: 706-692-9463
Pickens County Visitor Guide
Spectacular mountain views await you in Pickens County, Georgia, the gateway to the North Georgia Mountains!
Website: http://www.visitpickens.com
Phone: 706-692-5600
Tater Patch Players95 Philadelphia LaneView on Map
706-253-2800Tater Patch Players fabulous productions draw talent from several surrounding communities. The troupe will also host poetry readings and stage bistro theater. Summer... Find Out More
Bent Tree Stables40 Little Pine Mountain RdView on Map
706-692-6322Bent Tree Stables is a full-service equestrian center located within the gated community of Bent Tree. The stable operations are open to the public for lessons, boarding... Find Out More
Fainting Goat Vineyards and Winery201 Vineyard Wy.View on Map
706-692-9463Our boutique vineyard is nestled up against the southeast side of Burnt Mountain in picturesque Pickens County, Georgia. Fainting Goat is a family-owned vineyard and... Find Out More
Seventy-four Ranch Bed and Breakfast9205 Hwy. 53 W.View on Map
706-692-0123Get ready to relax in rugged indulgence at the Seventy-four Ranch Bed and Breakfast. The historic Butler-Cape House, built in the mid-1800s, is the heart of the ranch... Find Out More
Microtel Inn & Suites by Wyndham Jasper171 H. Mullins Ct.View on Map
706-299-5500Welcome to the newly renovated Microtel Inn & Suites by Wyndham Jasper hotel, near Amicolala Falls State Park. Located off Highway 5 / 515, our Jasper, GA, hotel offers... Find Out More
Seventy-four Ranch Bed and Breakfast9205 Hwy. 53 W.View on Map
706-692-0123Get ready to relax in rugged indulgence at the Seventy-four Ranch Bed and Breakfast. The historic Butler-Cape House, built in the mid-1800s, is the heart of the ranch... Find Out More
Woodbridge Inn Restaurant & Lodge44 Chambers St.View on Map
706-253-6293Owned and operated by the Rueffert family since 1976, this unique restaurant and hotel has been featured on Food Network, CNN, Georgia Traveller, The Doctors and several... Find Out More
Long John Silver's170 Bill Wigington PkwyView on Map
Kfc61 Melissa PkwyView on Map
Harvest Day Cafe408 E Church StView on Map
Subway1100 Old Philadelphia RdView on Map
The dining information on this page is supplied by the Georgia Restaurant Association.


text:
create a custom script for an incentive website
This project received 8 bids from talented freelancers with an average bid price of $574 USD.Get free quotes for a project like this
Project Budget$250 - $750 USD
Total Bids8
Project Description
Incentive Website Script Like FSRevolution Script
I am interested in buying a custom built script, but would prefer someone who knows what FSRevolution is. All the features that they used on that script I would like on this script. These include things like: Postback Crediting - Variable offer weights - Support ticket System Integration - Manual Credit Giving [url removed, login to view] user information - Admin/User "Inbox" - Adding Offers - Offer Credit & Referral Credit (you must first complete the offers before getting referrals to do the same) , and many others. You can see exactly all the features here:Look at their sites, and take a look at the features page, they have pictures of the admin side of the script - VERY IMPORTANT that you realize all these features.
I need to be able to add offers from affiliates through comission junction and similar sites, manually, or automatically. these offers need to each have a credit weight, which adds up to complete 1 full credit. 1 full credit gives the user a prize, which is chosen at sign up. This is the functionality at its simplest, now here are examples of these type of scripts running the sites, like the ones I would like to own:[url removed, login to view] (Runs FSRevolution You can check out how each works and see their similarities. Tde of these sites, I would prefer somebody who has done this before. Thank u
Skills Required
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online