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In cold climates, some plants and bacteria that cannot avoid freezing use antifreeze proteins (AFPs) to lessen the destructive effects of ice recrystallization. These AFPs have weak freezing point depression activity, perhaps to avoid sudden, uncontrolled growth of ice. Here, we report on an uncharacteristically powerful bacterial AFP found in an Antarctic strain of the bacterium, Marinomonas primoryensis. It is Ca2+-dependent, shows evidence of cooperativity, and can produce over 2 °C of freezing point depression. Unlike most AFPs, it does not produce obvious crystal faceting during thermal hysteresis. This AFP might be capable of imparting freezing avoidance to M. primoryensis in ice-covered Antarctic lakes. A hyperactive bacterial AFP has not previously been reported.
Antifreeze proteins (AFPs) are a diverse group of ice-binding proteins that inhibit the growth of ice in two different situations. Prior to freezing they possess thermal hysteresis (TH) activity, which is the non-colligative depression of the freezing point of a solution containing ice below its melting point. In the frozen state they show ice recrystallization inhibition (RI), whereby the proteins inhibit the growth of large crystals at the expense of small crystals at high subzero temperatures. AFPs have been found in animals [1,2], plants [3,,,,7], fungi [8,9] and eubacterial species [8,10,,12]. AFPs isolated from animals typically have substantial TH activity. This is consistent with these species having a freeze-avoidance strategy, whereby they depress the freezing point of their body fluids to prevent freezing of tissues [1,2,13,14]. However, AFP-producing plants and bacteria reported to date show substantially lower thermal hysteresis activity than do animals. This is consistent with a freeze-tolerance strategy whereby these organisms readily freeze but use the RI activity of AFPs to control the size of ice crystals [7,15]. The bacterial AFPs that have been assessed for TH, have demonstrated low activity [8,10,,12] compared to insect and fish AFPs, which have up to 5 and 2 °C of TH, respectively [1,16,,18].
In a previous study, Gilbert et al. [19] isolated 11 bacterial strains with RI activity from several Antarctic lakes in the Vestfold Hills, Eastern Antarctica (68°S, 78°E). A bacterium identified as M. protea in the original study [20] is now thought to be a strain of Marinomonas primoryensis [21] based on 16S rDNA comparison (results not shown). It was one of the most abundant AFP-active bacterial strains isolated in that study. The lakes from which M. primoryensis was cultured were isolated from the sea following the last glacial maximum around 10,000 years ago when the land rose up as the ice cap retreated [22]. They are approximately half as saline as seawater, and ice-covered, usually with a short ice-free period of 3–6 weeks in late summer. Their brackish salinity helps maintain the water temperature in the water column between −1 °C (at the ice–water interface) and +1 °C (at the base of the oxygenated water) [19], thereby constituting an ice-laden but thermally buffered environment. This study describes the preliminary characterisation of a novel, hyperactive AFP from M. primoryensis (MpAFP), revealing several unusual properties, including Ca2+-dependence.
Materials and methods
Protein preparation
M. primoryensis was cultured in 1 l of 50% sea water broth (SWB) growth medium (per L: yeast extract (Sigma) 1 g, peptone (Sigma) 1 g, sea salt (Coral Life, Aquatics Online) 19 g), with shaking at 4 °C for 4–5 days, or until the optical density at 600 nm reached 0.8–0.9. The cultures were centrifuged (4500×g at 4 °C for 15 min in a Beckman JA4.2 swinging bucket rotor). The cell pellet was resuspended in 2.5 ml of ice-cold lysis buffer (25 mM Tris/HCl (pH 8.0), 10 mM CaCl2). Following sonication on ice, the lysate was centrifuged (27,000 ×g (15,000 rpm) at 4 °C for 1 h in a Beckman JA20 rotor) and the supernatant (cell-free extract) was removed and stored at −20 °C.
Thermal hysteresis measurement and image capture
TH measurements were made using a Clifton Nanolitre Osmometer (Clifton Technical Physics) as previously described [23]. To study the effect of [AFP] on TH activity, serial dilutions of the lysate supernatant were made in lysis buffer. Digital images of ice crystals formed during TH assay were taken using a Nikon COOLPIX 4500 digital camera mounted on a Leitz dialux 22 microscope with a Leitz Wetzlar 160/- EF 10/0.25 objective.
Determining Ca2+-dependency
To determine the dependency of MpAFP activity on divalent metal ions, samples containing MpAFP were subjected to exhaustive dialysis against 25 mM Tris/HCl (pH 8.0), from which metal ions (e.g., Ca2+) had been chelated by passage through a Chelex-100 chelating resin column (iminodiacetic acid immobilised on 1% cross-linked polystyrene 50–100 dry mesh), (Sigma) at 0.5 ml min−1. Approximately 10 ml of crude lysate was dialysed in 3500 MWCO (molecular weight cut-off) dialysis tubing (Fisher) at 4 °C against 1 l of Ca2+-free 25 mM Tris/HCl (pH 8.0) for ?15 h, with three changes of the dialysis buffer. Following dialysis, metal salts (CaCl2, MnCl2, MgCl2, ZnCl2, CuSO4, NiSO4) were individually added to the solution to a final concentration of 10 mM to determine the effect of each upon the TH activity of the dialysed lysate supernatant.
The effects of proteolysis and chelation
The effects of proteases on TH activity were determined as follows. Pronase and trypsin (Sigma) were used at 37 °C at a 1:10 enzyme to substrate ratio. The protein concentration of the crude lysate supernatant was determined by Bradford assay (Bio-Rad) and a concentration of ?6.5 mg ml−1 was used for these experiments. Aliquots of the digests were measured for TH activity every 30 min. Proteinase K (MBI Fermentas) was added to the crude lysate supernatant to a final concentration of 2 mg ml−1 and incubated at 37 °C. TH activity again was recorded every 30 min. All protease experiments were performed in the presence and absence of Ca2+, where Ca2+ was removed from crude lysate supernatant by dialysis as described above. The effects of EDTA, EGTA and O-phenanthroline on MpAFP TH activity were recorded following incubation on ice for 1 h.
Thermal hysteresis activity of crude lysate and growth medium
A typical preparation of crude lysate supernatant contained 11 mg ml−1 of protein and had an activity of 0.8 °C (±0.07 °C). There was little variation in concentration or activity between preparations. No activity was found in the growth medium recovered after pelleting the cells, even after 100-fold concentration.
Characterisation of MpAFP
An initial attempt to purify the MpAFP by size-exclusion chromatography of lysate supernatant resulted in the complete loss of TH activity. From this, we reasoned a low molecular weight factor was required for activity (not shown). This result was confirmed when extensive dialysis of the lysate supernatant in 3500 MWCO membranes also caused the complete loss of activity (Fig. 1). The addition of 10 mM CaCl2 to the dialysate restored full activity. The addition of other divalent metals ions (Mn2+, Co2+, Ni2+, Zn2+, Cu2+, Mg2+) to the Ca2+-free dialysate had no effect on activity.
The effect of chelating agents on MpAFP activity in crude lysate supernatant was investigated (Fig. 1). Addition of 10 mM EDTA or EGTA on ice caused a 50% reduction in TH activity. Addition of 100 mM EDTA also caused a 50% reduction in activity. The saturation of 10 mM EDTA with 20 mM CaCl2 fully restored the activity, confirming that loss of activity with EDTA is due to chelation of Ca2+ ions. Addition of the iron chelator O-phenanthroline to 10 mM had no effect on activity.
The effect of proteolysis on MpAFP activity of the crude lysate supernatant, was tested with and without Ca2+ present (Fig. 1). Pronase, in the presence of Ca2+, caused a decrease in TH activity of 0.09 °C, for every 30 min of incubation at 37 °C (not shown). In the absence of Ca2+, activity was extinguished within 30 min. Proteinase K was more detrimental, eliminating activity within 30 min in the presence or absence of Ca2+. Trypsin had no effect on activity in the presence of Ca2+. There was no loss of activity following incubation at 37 °C with trypsin for 6 days; thereby indicating the thermal stability of MpAFP at 37 °C when Ca2+ is present. However, in the absence of Ca2+, activity was completely extinguished by 30 min.
Effect of dilution on crude lysate supernatant TH activity
Two-fold serial dilutions of this cell-free extract were prepared and assayed for TH. The plot of activity against protein concentration (Fig. 2) showed a sigmoidal relationship at low protein concentrations. TH activity was undetectable below 0.18 mg ml−1 but began to increase rapidly at 0.36 mg ml−1 and followed a more hyperbolic relationship with protein concentration from there on. The crude bacterial lysate supernatant was quite active in comparison to pure type III AFP. Concentrating the cell-free extract approximately 20-fold using a 30,000 MWCO Amicon ultra centrifugal concentrator (Millipore) increased the activity to >2 °C, higher than the maximal activity of most fish AFPs.
Ice crystal morphology
Ice crystals formed in the presence of MpAFP do not have distinct facets (Fig. 3(a) and (b)). They are typically rounded in shape and do not change their morphology during the course of the TH measurement. The ice crystal "burst" occurring at the end-point of TH is dendritic with hexagonal symmetry, suggesting growth from primary or secondary prism faces or edges (Fig. 3 (c)). This more closely resembles the "bursts" seen with insect AFPs than "bursts" that occur along the c-axis from crystals inhibited by fish AFPs [24].
In this study, we have partially characterised a Ca2+-dependent, bacterial AFP that demonstrates hyperactive thermal hysteresis. Previously, bacterial AFPs have demonstrated RI activity but only low levels of TH activity (<0.1 °C), e.g., Micrococcus cryophilus & Rhodococcus erythropolis [8], Pseudomonas putida [10], Moraxella sp. [11] and Pseudomonas fluorescens [12]. The presence of an AFP with low TH activity would suggest that these bacterial strains employ a freeze-tolerant strategy similar to that of plants, e.g., rye grass [6], carrot [4], winter rye [25] and bittersweet nightshade [3]. The presence of a highly active bacterial antifreeze in M. primoryensis suggests that some bacteria, like insects [26,27] and fish [2] have also evolved a freeze-avoidance strategy, which has not yet been reported in microorganisms.
As this strain of M. primoryensis was isolated from a saline (1.9%), permanently cold (−1 to +1 °C) Antarctic lake with 1–2 m deep ice cover [19], we hypothesise that this bacterium has evolved a highly active AFP to resist freezing in the water column. An AFP with TH activity comparable to that found in marine fish should be sufficient to inhibit the growth of ice crystals that might otherwise be propagated into the bacterium, especially near the ice–water interface of the lake. The other AFP-active bacterial species were isolated from harsher, frozen environments, i.e., high Arctic plant rhizosphere (P. putida [10]), mid-gut of frozen beetle larvae (R. erythropolis), frozen/chilled pork sausages (M. cryophilus [8]), and Antarctic soil (Moraxella sp [11] and P. fluorescens [12]). Bacteria in these environments would require an RI-active protein to prevent lethal recrystallization. Even the most potent AFPs are unlikely to prevent a bacterium from freezing in these harsh environments, and high TH activity will tend to cause rapid, uncontrollable ice crystal growth when freezing inevitably occurs.
Most previously reported bacterial AFPs (see above) were shown to be exported from the cell [8,10,,12]. However, in M. primoryensis AFP activity was only found in the cellular lysate supernatant. While we have not yet determined the location of MpAFP within the cell, we suggest that it would be most effective if localised to the periplasmic space, where it would be likely to encounter, bind and inhibit embryonic ice crystals from the extracellular environment before they damage the cell.
Many AFPs impart a characteristic morphology to ice crystals as a result of their crystal plane-specific binding. For example, fish AFP type I from winter flounder binds to the {20–21} pyramidal planes of the ice crystal, forming hexagonal bipyramids [28]. This morphology is assumed on initial cooling at the start of the TH-gap (temperatures between non-equilibrium melting and freezing points). Below the non-equilibrium freezing point, fish AFPs tend to exhibit uncontrollable growth along the c-axis [24]. In comparison, the insect AFP from Choristonerua fumiferana binds to both the primary prism planes and basal planes, to produce a hexagonal plate morphology that is stable throughout the TH-gap. The structurally similar but unrelated AFP from Tenebrio molitor produces an even more complex lemon-shaped crystal morphology [16] suggesting possible binding to more than just the basal and primary prism planes. For both insect AFPs, uncontrollable ice growth below the non-equilibrium freezing point occurs primarily along the a axes, producing a dendritic crystal pattern with hexagonal symmetry [29]. This "burst" pattern is also seen with MpAFP. Other bacterial AFPs have been shown to produce hexagonal crystal shaping [10,,12], but as yet their binding plane specificity has not been reported.
Unlike most AFPs, which show a hyperbolic relationship between TH activity and AFP concentration [30], the sigmoidal shape of the dilution curve at low protein concentrations of MpAFP is suggestive of cooperativity in binding to ice or inhibition of its growth. A similar result has been reported for one of the smaller AFGPs [17].
To date, Ca2+-dependent AFP activity has only been shown in fish AFP type II from rainbow smelt (Osmerus mordax) and Atlantic herring (Clupea harengus) [31]. The herring AFP, like MpAFP, demonstrates a conformational change from a protease-sensitive, inactive form to a protease-resistant, active form with the addition of Ca2+ [31]. Type II AFPs are homologous to the carbohydrate-recognition domain of the Ca2+-dependent (C-type) animal lectins [32]. Although there are lectins in bacteria that bind Ca2+ [33], there is no evidence at this time that MpAFP is a homolog. Stressman et al. [34] noted that during freeze/thaw cycling, the AFP from winter rye (Secale cereale) bound Ca2+ to the detriment of its activity. However, if the AFP was maintained at 4 °C, Ca2+ had no effect on its activity. We note that the bacterial AFP from P. putida, AfpA, contains a series of putative Ca2+-binding repeats that could be involved in a possible hemolysin-like, Ca2+-binding secretion domain [35]. However, there have been no reports as yet of Ca2+-dependent bacterial AFPs. As such the Ca2+-dependency in MpAFP is novel for AFPs from this kingdom.
In summary, we report the discovery of a Ca2+-dependent, hyperactive bacterial antifreeze protein from an Antarctic lake strain of the bacterium M. primoryensis.
This work was funded by a grant from the Canadian Institutes of Health Research (CIHR). P.L.D holds a Canada Research Chair in Protein Engineering. We are grateful to Chris Marshall and Dr. Andrew Scotter for comments and suggestions.

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Someone back then asked why I cared about "slow cars". Told them that they were the "Pro" versions of the cars I actually drove. "Why does a summer league player watch Major League Baseball?", I said. Ol' ex-Improved Touring driver wishes he was half as talented as these guys (other SCCA members will know the names!).
1991 SCCA Escort World Challenge at Sears Point (partial)
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Scooby-Doo's Haunted Mansion Ride
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|Property type
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When you book through the HomeAway checkout, your booking is backed by our Book with Confidence Guarantee™ offering comprehensive payment protection.Learn more
In the main hall room for approx. 20 persons to conference and dine. In the lobby are room to gather and to have small group meeting while a separate cabinet gives a private conference room for 6-8 persons. PC-projector, internet with wifi, flip board, two large 47 inch flat TVs with PC-connection.
Set of dishes for up to 50 persons, 2 electric stoves, fridge, freezer, micro wave, dish machine.
Entrance lobby offers room for approximately 10 persons to gather, to drink and eat or just to relax after the soft & hot sauna experience.
Bed places for approx. 30 persons. 18 standard beds in two big bedrooms for 6 people each and one bedroom for 5 persons, plus addiditional extra beds can be arranged for up to 10 people. Even bigger groups can be accommodated in case some people are willing to sleep on the mattresses. We have the space at Ruuhijärvi Lake!
Electrically heated sauna, dressing room, 3 showers, WC, sauna has room for 4-5 persons..
Two wood heated saunas with separate dressing rooms, two wood-heated heating cauldrons, Both saunas have room for 8-10 persons, in total for up to 20 persons.
Our old times timber made cottage at the Ruuhijärvi yard has space for approx. 40 persons and can be used for celebrations or for old fashion dining room and meetings. The wood-heated massive stone stove gives an atmosphere from hundred years ago.
PLEASE NOTE: calendar is not updated. We wish to serve every group according to the size of the group and special needs. Therefore, please always contact the owner for your detailed offer. Please see www.evonature.fi and contact the owner: email@example.com or call +358404173627.
Evo Nature – family owned little resort with peaceful lake in the southern Finland with great access to major Finnish cities.
Peace and silence in wilderness but still very close, 139 km from Helsinki airport, 220 km from the Russian border.
All destinations have their own lakes, purely reserved for our guests, just peace and silence.
Have a meeting, go fishing or hiking, take vacation, hike or just relax the way you or your customers want to. Easy going meetings, celebrations, parties or family holidays. Two hidden lakes, saunas and swimming facilities. All ready waiting for you.
Just hour and a half from Helsinki and one hour from city of Tampere. Explore our web site and you will see how close the wilderness can be!
Evo Nature, peace of mind all year around.
If you have a large group and want to have extremely peaceful surroundings with cosy and relaxing atmosphere, Lake Ruuhijärvi is the place.
Wonderful place for friends to come together, for several families or generations, for festive occasions.
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The buildings are very well kept though built originally nearly 100 years ago. Now we offer all modern facilities in 'the middle of nowhere'.
There is no similar place on Earth!
Ruuhijärvi facilities are located within the Evo National park and hiking area.
Though Evo hiking area is within easy reach of Finland's largest cities, you still feel you are in the heart of the forest here. Ramble along easy trails or take a longer hike. Evo's forests are home to wildlife including elk, lynx and beavers. Facilities by Evo's excellent fishing waters have been designed to make them comfortably accessible to fishers of all ages. Evo is a great place to bring the whole family for an adventure.
Evo Hiking Area is just a short drive away from the big cities of Southern Finland. The area offers many kinds of nature-based adventures: pick your favourite from activities such as hiking, fishing, skiing and hunting.
Evo Hiking Area is an excellent fishing destination for beginners and seasoned fishermen alike, and therefore ideal for families. If you want to fish from a boat, there is a boat hire spot in the Niemisjärvet fishing zone. The lakes and ponds in the area are regularly stocked with trout. Lake Vähä-Koukkujärvi is reserved for fishermen aged 12 or under. Fishing on Lake Syväjärvi is reserved for visitors staying at the rental cabin there. There are also many natural lakes and ponds that are suitable for lure fishing and angling in the area.
History and Sights of Evo Hiking Area
Evo has been inhabited since the 1300s, but there is very little information available about the early days. In the 1800s, when the general parcelling out of land was carried out in Finland, the forests of Evo became surplus land of the state, and they were logged without pity for tar burning and to hew planks. Slash-and-burn agriculture was also common.
Natural Features of Evo Hiking Area
Evo is located in a watershed region, higher than its surroundings. Small lakes and soil low of nutrients are typical in the landscape. Traditional forestry has affected the development of the natural features in Evo. Moist and dry forests are the dominant forest types. The Pine (Pinus sylvestris) is the most common tree species, more than half of the forests in the area are pine forests. There are not many lush places in Evo. Also mires in their natural state are rare. Along Niemisjärvi Nature Trail or Harjupolku Nature Trail, the hiker can learn about mires.
The hilly landscape was shaped by the Ice Age. The highest altitude in the area is 160 metres above the sea level on Syrjänalusenharju Ridge, the tops of which have never been under water.
The Moose (Alces alces) is common in the forests of Evo, especially in the winter. The emblem animal of the area, the Lynx (Lynx lynx) is very occasionally seen in Evo, but its pawprints can be found on the snow. Other interesting mammal species found in the area are the Canadian Beaver (Castor canadensis) and the Siberian Flying Squirrel (Pteromys volans). The Beaver lives in brooks, and the Flying Squirrel in spruce-dominated mixed-wood forests, where old Aspens (Populus tremula) grow.
Of tetraonids living in the area, the most common is the Hazel Grouse (Bonasa bonasia), and the Western Capercaillie (Tetrao urogallus) and the Black Grouse (Tetrao tetrix) can also be seen. The nesting bird species of the many small lakes of Evo include the Common Goldeneye (Bucephala clangula), the Black-throated Diver (Gavia arctica) and several hawk and owl species. The Red-throated Diver (Gavia stellata) and the Osprey (Pandion haliaetus) are rare nesting species. Swans and a few other species of anseriformes stop to rest in Evo during their migration.
The most common fish species in Finland, such as the Pike (Esox lucius), the Perch (Perca fluviatilis), the Burbot (Lota lota) and the Roach (Rutilus rutilus), live in the small forest lakes of Evo. In the recreational fishing area Rainbow trouts (Oncorhynchus mykiss), Arctic chars (Salvelinus alpinus) and Trouts(Salmo trutta) have been planted.
|Rate Period
|Weekend Night
|Monthly *
My Standard Rate
2 night minimum stay
|Pet Fee
|Tax Rate
Please note that above listed pricing is a starting price during the low season (November, January and February).
PLEASE ALWAYS ASK FOR DETAILED OFFER. Please see details at www.evonature.fi and contact the owner: firstname.lastname@example.com or +358404173627.
We wish to serve every group according to their needs and expectations and therefore we tailor pricing according to the size of the group, per season, per time of the week and per length of the reservation.
PLEASE ASK FOR AN OFFER: firstname.lastname@example.com or please call us +358404173627
In order to provide a detailed offer please inform us with estimated number of guests, number of days and desired period.
* Approximate monthly rate. Actual rate will depend on the days of the month you stay.


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Aliyah to Israel: Immigration under Conditions of Adversity
A snapshot at figures of immigration (Aliyah) to the Land of Israel (Palestine) and to the State of Israel reveals the following: between 1882-1947, in successive waves of immigration, some 543,000 Jews immigrated to Palestine, joining the 24,000 who lived there. During the first three years of statehood (1948-1950) the average annual growth rate of the Jewish population was about 24 percent, and between 1948-1952, mass immigration of 711,000 supplemented a population of 630,000. Recently, Israel witnessed a massive influx of Soviet immigrants. During 1990-1998 the Israeli population of 4.56 million was enriched by 879,486 immigrants, a growth rate of 19.3 percent. In 1991, 15,000 Jews were airlifted in one single day in "Operation Solomon". What were the factors that drove this unprecedented migration of Jews from around the globe to Israel? Many of the major international migration movements were largely economic in nature (the push of poverty or the pull of expected enhanced standards of living) or have been in response to persecution. While all these factors have played some role in immigration to Israel, the Israeli case is unusual in that its origins are essentially ideological. Israel has always encouraged and assisted the immigration and absorption process as part of a pro-immigration ideology and policy. Its raison d'être was and remains the ingathering and retention of Jewish immigrants and the forging of these diverse elements into a unified nation. Israel is a country established for and administered by immigrants from diverse countries and origins. In most cases of rapid population growth, per capita income declines. This is due to adjustment problems, low productivity of immigrants and infrastructure bottle-necks. Israel was an exception. Immigration was accompanied by accelerated growth rates even though large public funds were devoted to housing, employment and social services to facilitate the direct absorption of mass immigration. As a result, per capita income which was $3,500 in 1950 surged to $17,000 in 1996 - an annual growth rate of 3.5 percent. This paper describes the major waves of immigration to Israel, starting from 1882 up to the present. For each immigration wave, its size, composition, origin and characteristics are documented. It then focuses on the process of immigrants' assimilation in the local labor market and addresses three main questions: (i) How well do immigrants adapt to the Israeli economy? (ii) What are the effects of immigration on employment opportunities and are they paying their way in the welfare state? (iii) Are population and production growth interrelated? The facts and figures lead to an overall evaluation of the immigration process and to the lessons to be learned from past experience to improve the absorption process. In particular, return migration, which reflects failure to absorb, is discussed, and the very different absorption policies of the 1950s and the 1990s are contrasted and compared. Suggestions for changes in immigration policies are discussed. An evaluation of the contribution of immigration to economic growth and of the importance of Hebrew language acquisition is also presented. The last section of this paper provides a summary and conclusions.
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|64 pages
|Date of creation:
|Dec 1999
|Publication status:
|published in: Zimmermann, Klaus F. (ed.), European Migration: What Do We Know, 2005, Oxford: Oxford University Press, 459-506
|Contact details of provider:
| Postal: IZA, P.O. Box 7240, D-53072 Bonn, Germany
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Our Mutual Friend
By (author) Charles Dickens
Edited by Michael Cotsell
Format: Paperback
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Our Mutual Friend by Charles Dickens
Book DescriptionFollowing his father's death John Harmon returns to London to claim his inheritance, but he finds he is eligible only if he marries Bella Wilfur. To observe her character he assumes another identity and secures work with his father's foreman, Mr Boffin, who is also Bella's guardian. Disguise and concealment play an important role in the novel and individual identity is examined within the wider setting of London life: in the 1860s the city was aflame with spiralling financial speculation while thousands of homeless scratched a living from the detritus of the more fortunate-indeed John Harmon's father has amassed his wealth by recycling waste. This edition includes extensive explanatory notes and significant manuscript variants. ABOUT THE SERIES: For over 100 years Oxford World's Classics has made available the widest range of literature from around the globe. Each affordable volume reflects Oxford's commitment to scholarship, providing the most accurate text plus a wealth of other valuable features, including expert introductions by leading authorities, helpful notes to clarify the text, up-to-date bibliographies for further study, and much more.
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Book DetailsISBN: 9780199536252
(195mm x 129mm x 40mm)
Pages: 880
Imprint: Oxford University Press
Publisher: Oxford University Press
Publish Date: 13-Nov-2008
Country of Publication: United Kingdom
Other Editions...
Books By Author Charles Dickens
Our Mutual Friend, Paperback (April 2017)
Mistakenly assumed to have drowned, John Harmon assumes a new identity to get acquainted with the couple next in line for his inheritance and the woman his father's will stipulates he must marry.
Bleak House, Paperback (April 2017)
The beloved storyteller drew upon his own experiences as a law clerk and plaintiff to enliven his satire of the English judicial system, a richly plotted tale of secrets, murder, and mystery.
Great Expectations, Paperback (January 2017)View all books by Charles Dickens
Perhaps Charles Dickens' most famous novel, and a stunning coming of age story featuring young Pip, who goes from rags to riches - but at what cost?
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Officials destroy 6 tons of ivory
COMMERCE CITY, Colo. U.S. officials on Thursday destroyed more than 6 tons of confiscated ivory tusks, carvings and jewelry – the bulk of the U.S. "blood ivory" stockpile – and urged other nations to follow suit to fight a $10 billion global trade that slaughters tens of thousands of elephants each year.
Thousands of ivory items accumulated over the past 25 years were piled into a large pyramid-shaped mound, then dumped into a steel rock crusher that pulverized them into dust and tiny chips at the National Wildlife Property Repository north of Denver.
To raise awareness further, officials said, the crushed ivory would be used to create memorials against poaching. The United States is the second-largest consumer, behind China, of illegal animal products such as elephant ivory, rhinoceros horn and tiger blood.
"These stockpiles of ivory fuel the demand. We need to crush the stores of ivory worldwide," said Dan Ashe, director of the U.S. Fish and Wildlife Service. He said keeping stockpiles intact can feed consumer demand for illegal souvenirs and trinkets taken from slain elephants.
The Philippines, Kenya and Gabon also have destroyed their ivory stocks.
Before the crush, Fish and Wildlife officials showed off thousands of confiscated ivory tusks, statues, ceremonial bowls, masks and ornaments – a collection they said represented the killing of more than 2,000 adult elephants.
The items were seized from smugglers, traders and tourists at U.S. ports of entry after a global ban on the ivory trade took effect in 1989.
The message from Thursday's crush likely will reach consumers more than the faraway poachers and smugglers.
While poaching once was largely a crime of opportunity, officials say it has become increasingly sophisticated, often orchestrated and carried out by heavily armed people, connected to larger criminal groups, who do not hesitate to kill those who try to stop them. The cash goes to fund other crimes such as human trafficking and conflicts in the region.
"These people are also a threat to international order," said Robert Dreher, acting assistant attorney general for the environment and natural resources.
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Gamebreaker.tv: Guild Wars 2 Launch Date Announcement "Imminent"?
Discussion in 'GW2 General Discussions' started by Zosimus, Jun 14, 2012.


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IP Cam
By Senstic
Open the Mac App Store to buy and download apps.
IP Cam turns your mac into a IP surveillance camera (with audio). Run IP Cam on your mac, and via Wi-Fi network, you can view live video and audio captured by your mac's built in FaceTime camera (or any mac compatible USB/Firewire camera) on web browser from any computer. You can even view IP Cam using Safari browser from another mobile device such as iPhone, iPad or iPod Touch.
IP Cam supports standard MJPEG streaming, so you can view it from any other IP Camera viewer apps.
IP Cam also supports audio capture, so you can remotely listen to the audio captured by mac's microphone while viewing live camera.
* Live video and audio capture from mac.
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Fixed a crash issue on certain older mac models.
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Neat but not covert
It does work, but has a visible dock icon and task try icon. As such, it's not good for covert monitoring. A casual user would quickly figure out what it is and disable it. Nice for watching your cats or other willing participants.
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Compatibility: OS X 10.8 or later, 64-bit processor


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Cowboys get past Packers
Marion Barber rushed for 142 yards with a touchdown on 28 carries Sunday night, as the Dallas Cowboys defeated the Green Bay Packers 27-16.The Cowboys were a 3-point road favorite in the game and were able to cover the spread. The combined score fell UNDER the posted over/under total (51).
Tony Romo passed for 260 yards with a touchdown and an interception for Dallas and Miles Austin caught two passes for 115 yards with a touchdown.
Aaron Rodgers threw for 290 yards with a TD run for Green Bay, while Greg Jennings hauled in eight passes for 115 yards.


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Lot 325: George Roby ceramic sculpture
Fine Art at Auction
by Rachel Davis Fine Arts
October 22, 2016
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Description: George Roby (American 20th c.)- Untitled- ceramic sculpture, signed with initials. 21 x 18 x 18''
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An excellent article by Judon Fambrough of the Real Estate Center at Texas A&M University, about how oil and gas leases can be extended beyond there primary term, can be found here. Great tips about how to avoid pitfalls in lease terms. Mr. Fambrough has written many good articles about negotiating oil and gas leases.
Articles Posted in Negotiating Leases
Thoughts on the Oil and Gas Lease
Last week I presented a paper at the Texas State Bar Advanced Real Estate CLE Conference for attorneys in San Antonio. I was asked to write a paper giving real estate attorneys a basic introduction to negotiating oil and gas leases. It might seem odd that real estate attorneys would want a primer on oil and gas leases; most people would assume that an attorney practicing real estate law in Texas would know about oil and gas leasing. And that used to be true, when the majority of attorneys had a rural general practice. General practitioners in Texas knew the basics of real estate and oil and gas law and often helped their landowner clients negotiate leases. Today, most real estate attorneys have little to do with oil and gas matters, and as practices have become more specialized the oil and gas specialty has diverged from the real estate specialty.
I was given thirty minutes to make my presentation – hardly enough time to do justice to the subject of oil and gas leases. The exercise of preparing my remarks caused me to focus on some basic concepts that I've not recently thought about, and I decided they would make a good topic for discussion here.
The oil and gas lease is in many ways a unique form of contract. It is the foundation of the oil and gas industry in the U.S. Because most minerals in the U.S. — unlike most of the world — are privately owned, some way had to be found for those willing to risk capital to exploit oil and gas to obtain rights to those resources. The oil and gas lease was the result. In its basic form, the oil and gas lease has remained unchanged since the early days of the industry.
Lease Ratifications
I have recently been asked to review requests for lease ratifications sent to my clients, and I thought that ratifications would be a good topic for this site.
Companies generally ask owners of royalty and non-executive mineral interests to ratify oil and gas leases covering the lands in which they own an interest. The companies ask for the ratification because they want the right to pool the royalty or non-executive mineral interest covered by the lease. In Texas, even though the holder of the executive right (the right to lease) has the right to negotiate and grant leases covering the interests of royalty and non-executive mineral owners, the holder of the leasing right does not have the right to grant the lessee the right to pool those interests (unless that right was expressly granted or reserved in the instrument creating the royalty or non-executive interest). In order for a pooled unit to be effective as to a royalty or non-executive mineral owner's interest, the owner must either agree to the pooled unit or grant the lessee the right to pool his/her interest.
A non-executive mineral owner is the owner of a mineral interest who has given up the right (by conveyance or reservation) to lease his/her interest. The non-executive mineral owner has the right to receive his/her share of any bonus and royalty paid pursuant to the lease granted by the holder of the leasing right.
Dealing With Landmen
Ian Urbina, the New York Times reporter who has written several articles recently about oil and gas exploration and the perils of hydraulic fracturing, recently wrote an article, "Learning Too Late of Perils in Gas Well Leases," that appeared on the front page of the Times on December 2. In research for the article the Times obtained and reviewed more than 111,000 oil and gas leases covering lands in Texas, Maryland, New York, Ohio, Pennsylvania and West Virginia – a remarkable effort. Urbina's article points out several ways in which the leases fail to protect the interests of landowners:
— They do not require companies to compensate landowners for water contamination.
— They do not address well locations, destruction of trees, or other surface use issues.


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Full profile →'">
The author is a Forbes contributor. The opinions expressed are those of the writer.
While Sheldon Adelson's support of Newt Gingrich has drawn more media attention, other billionaires have quietly lined up behind Mitt Romney for the Republican presidential nomination.
A filing Tuesday by Restore Our Future, the super PAC working for Romney's election, shows that of 200 people contributing a total of about $30 million in the last six months of 2011, 10 were billionaires. They contributed more than $2 million to the PAC.
Adelson and his wife, Miriam, each contributed $5 million to the the Winning Our Future super PAC, which is supporting Gingrich.
The biggest billionaire contributor to the Romney group was investor Julian H. Robertson Jr., founder of Tiger Management, who gave $1 million.
Second on the list was William I. Koch, often referred to as "the other Koch brother." He gave $250,000.
Paul Tudor Jones II, Tudor Investment Corporation founder, gave $200,000.
Wal-Mart heirs Alice Walton and brother Jim Walton each contributed $100,000. Carolyn Rowan, wife of Apollo Global Management founder Marc J. Rowan, contributed $30,000.
Here's the full list of billionaire donors to the Romney super PAC:
|Kenneth C. Griffin
|Paul Tudor Jones II
|William I. Koch
|Julian H. Robertson Jr.
|Stephen M. Ross
|Steven Roth
|Marc J. Rowan
|Alice L. Walton
|Jim C. Walton
|Sam Zell
Follow me on Twitter: @Muckety.


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Information technology -- Small Computer System Interface (SCSI) -- Part 151: Serial Attached SCSI - 1.1 (SAS-1.1)
(Not available in French)
This standard was last reviewed and confirmed* in 2015.
ISO/IEC 14776-151:2010(E) defines the rules for exchanging information between SCSI devices using a serial interconnect. It defines the rules for exchanging information between ATA hosts and ATA devices using the same serial interconnect. It is a functional description. Conforming implementations may employ any design technique that does not violate interoperability.
Document published on: 2010-07 Edition: 1 (Monolingual) ICS: 35.200 Status: Published Stage: 90.93 (2015-12-21) TC/SC: ISO/IEC JTC 1/SC 25 Number of Pages: 559
This standard has not been revised
No corrigenda or amendments available


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All posts tagged Hilton
Hilton Hotels & Resorts launches in prime Edinburgh locale
In response to the rising needs of tourists and travellers, Hilton Hotels & Resorts has announced the opening of Hilton Edinburgh Carlton – the eighth Hilton Hotels & Resorts hotel in the country. More than four million tourists visit Edinburgh […]
Hilton Worldwide to develop its first modular hotel at Bristol Airport
Hilton Worldwide has announced plans to open the first hotel at Bristol Airport under its economy Hampton by Hilton brand, the Airport said
Hilton Birmingham Metropole refurbishes bar for guests, business customers
Hilton Birmingham Metropole, the largest conference and events hotel outside London, has announced the completion of a redesign and refurbishment of its bar area - The Lounge -
Hilton London Heathrow Airport launches 'Holiday Starts Here' package
Hilton London Heathrow Airport Terminal 5 has launched its 'Holiday Starts Here' package, designed to offer travellers a trouble-free start to holidays. Intended to help guests avoid the early
Hilton London Canary Wharf celebrates 'Tomorrow's Travel Leaders'
Hilton London Canary Wharf is celebrating 'Tomorrow's Travel Leaders' as Paul Middleton, its sales manager, has been invited to join the event launched by travel trade magazine, Travel
First DoubleTree by Hilton in Ireland Welcomes Guests
The Morrison Dublin, a DoubleTree by Hilton hotel, has opened in Ireland, bringing the upmarket, Doubletree, hotel brand to Ireland for the first time. Hilton Worldwide, the brand owner,
DoubleTree by Hilton Announces New Hotel in Scotland
The DoubleTree by Hilton hotel brand is to open its new DoubleTree by Hilton Dundee hotel in Scotland, after Hilton Worldwide, the brand owner, entered into a franchise
DoubleTree by Hilton Opens New Hotel in London
DoubleTree by Hilton brand has opened its newest London hotel property, DoubleTree by Hilton London - Marble Arch, close to Marble Arch station. The hotel, which was recently officially
Hilton London Heathrow Airport Terminal 5 Launches Shuttle Bus Service
Hilton London Heathrow Airport Terminal 5 hotel in London, UK, a brand from Hilton Hotels and Resorts, has introduced a complimentary shuttle bus service for its business guests. The
New Hilton Hotel to Open in Hampshire
Hilton Worldwide, a US-based hotel company, has announced a new Hilton at the Ageas Bowl cricket ground, Southampton, in England. The hotel will be a franchise of the brand,


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Imagine the worst. Suppose that you discovered that a member of your family was actually a criminal. Not a petty criminal, mind you, but one whose crimes were truly awful, morally repugnant in every sense. What would you do?
When David Kaczynski realized that his brother Ted was the Unabomber, he made the hard choice and called the FBI. Thanks to David's moral courage, the man responsible for murdering and maiming dozens of people with homemade bombs sent through the mail was stopped before he could carry out his grander schemes of putting bombs on airliners and detonating them over cities. When Kaczynski received a million-dollar reward from the FBI, he donated most of the money to his brother's victims.
Kaczynski had no special moral training to prepare him to turn in his brother or make other wrenching decisions. Until then, his life's work had been helping his father run a foam rubber business. It is therefore instructive to contrast his actions with those of the Pope, a man supposedly chosen by God to lead the world in the path of righteousness, and of his ring of cardinals and bishops.
Faced with overwhelming evidence that the church has been harboring and actively shielding child molesters and rapists, the Vatican went into a defensive mode that would have done the Nixon White House proud. Speaking to the faithful in his Palm Sunday address, Pope Benedict said he would not be intimidated by "petty gossip."
Bear in mind that gossip means unsubstantiated rumors. Imagine how that made the thousands of victims of priest-attacks feel -- including the hundreds of deaf boys who were molested by the man in charge of their care, the Reverend Lawrence Murphy. Their stories are documented. You can read them for yourself. It's just that no one would pay much attention, because their abuser was a priest.
In fairness, I suppose the Pope may have meant that only the published reports, based on documents, implicating him in Rev. Murphy's escape from justice were "petty gossip." But less than a week later, the Pope's personal preacher stood before a mic and made the outlandish comparison of media "attacks" on the Pope to the "collective violence" suffered by Jews. Yes, criticizing the Pope's failure to bring child-molesters in his house to justice is a lot like murdering six million Jews. Anyone can see that.
Six weeks later -- and centuries too late -- the Pope has made a very different speech. He blamed sin within the church for the crisis. That turnabout is commendable. So are his efforts to convey apologies to victims.
Yet the church has not entirely changed its longstanding policy of refusing full cooperation with law enforcement and is mounting the most vigorous defense possible against the claims of victims. Now you can't -- and I don't -- blame the church for defending itself. But in its pattern of using bankruptcy and arcane canonical legalisms to dodge settlements, it has shown all the compassion of a particularly nasty coal mine company toward its victims. And there's no sign yet of a change in that. Maybe it will come. Maybe not. But until the church does what any other legal institution harboring criminals and their abettors would do, such speeches amount to empty gestures of piety.
The Morning Email helps you start your workday with everything you need to know: breaking news, entertainment and a dash of fun. Learn more


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1. Take a pot and add parsnips to it.
2. Place the pot on heat and top them with stock. Bring the stock to boil and then turn down the heat. Make it really simmer. Allow it to cook for 15-20 minutes or until the parsnips becomes tender.
3. Drain stock from parsnips and put it in a food processer.
4. In the processor add butter, and a pinch of salt. Blend it well.
5. Add heavy cream to bring it to a perfect consistency.
6. Serve Mashed parsnip with chives garnish.
If the puree is too thick you may reserve the cooking liquid and use it to thin parsnips.
Serving size Per servingComplete recipe
Calories 194 Calories from Fat 103
% Daily Value*
Total Fat 12 g17.9%
Saturated Fat 6.8 g34.1%
Trans Fat 0 g
Cholesterol 31.2 mg10.4%
Sodium 174.3 mg7.3%
Total Carbohydrates 20 g6.6%
Dietary Fiber 4.4 g17.8%
Sugars 5.9 g
Protein 4 g7.3%
Vitamin A 6.6%
Vitamin C 26.1%
Calcium 4%
Iron 4.1%
*Based on a 2000 Calorie diet


text: The Chapel STL's Theatre Nuevo opens with a clown and balloons — and a serious mission By Nancy Fowler • Jul 23, 2015 Theatre Nuevo A clown, a poet, two children and two newscasters walk … onto a stage. It's not a joke (although it has jokes). It's a play called "This Is Not Funny," by a new company named Theatre Nuevo, opening tonight at the Chapel off Skinker near Forest Park. But the name's a contradiction, said founder and director Anna Skidis. Flute Flight concert combines talent, musicians give live studio performance By Katie Cook & Evita Caldwell • May 1, 2015 Kaitlyn Petrin Flute Flight is a duo performing Saturday in St. Louis that features the compatibility between conventional silver flutes and Native American style flutes.


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Are you looking for Second Trimester Abortion? Hotfrog showcases more than 60 popular businesses related to Second Trimester Abortion! To find more business related to Second Trimester Abortion, use the left navigation menu.
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10 things that explain Captain Phillips's capture and journey
Richard Phillips (left), who was captain of the Maersk Alabama when it was hijacked by Somali pirates in April 2009, is played by Tom Hanks (right) in the Paul Greengrass-directed film "Captain Phillips." Phillips, who was born in Winchester and lived in Boston, now lives in Underhill, Vt. Here is a 10-step explainer on the true story of how Phillips was returned home to New England after his capture; this feature does not focus on the events as they are portrayed in the movie. Next
The ship
The Maersk Alabama, pictured, was carrying cargo from Oman to Mombasa when it was hijacked by Somali pirates off the horn of Africa on April 8, 2009. Phillips was at the helm. It was the first American vessel be seized in 200 years. Next
The waters
The hijacking occurred during a rash of pirate activity in the Gulf of Aden and off the coast of Somalia. CNN has reported the controversey surrounding the film because of a recent lawsuit filed by members of the ship's crew that alleges that Phillips put their lives in danger by ignoring warnings and piracy reports and sailing so close to the coast of Somalia.
See a Boston.com interactive graphic from 2009 about pirate attacks in the Gulf of Aden Next
The seizure of the Maersk Alabama
Early on April 8, 2009, four teenage pirates were able to secure a grappling hook to the ship's deck, board the ship, seize the bridge, and capture Phillips. The ship is pictured here on April 11, 2009, after the pirates released it and an armed guard escorted it to the Kenyan port city of Mombasa. Next
The crew
Most of the ship's crew was below deck, where they were hiding in a fortified room from which they disabled the ship. When the pirate leader ventured down into the ship to find them, chief engineer Mike Perry grabbed him and held him hostage. The crew attempted to trade the pirate for their captain's release and the release of the ship but, after the pirate was handed over, the hijackers did not hold up their end of the deal. They left the ship with Phillips as their hostage; he allowed them to do so because it meant that his crew would go free.
The crew is pictured here on April 13, after the incident, speaking to media in the Kenyan sea port of Mombasa. Next
The lifeboat
Since the crew of the Maersk Alabama had destroyed the pirates' skiff using their ship's rudder, the original prisoner-trade deal included their leaving in the ship's lifeboat, a 28-foot enclosed boat with emergency food rations and medical supplies. They took the lifeboat, and Phillips with it. He was trapped inside the enclosed lifeboat with the four hijackers for five days. When tension mounted, conditions became unsanitary, and the heat became unbearable, Phillips tried to swim to safety, but was captured again. Now, you can go see the actual lifeboat at the Navy Seal Museum in Fort Pierce, Fla.
In this photo, the lifeboat is being towed by the USS Bainbridge following the rescue operation. Next
The US Navy's response
The Navy had several ships in the area, including the guided-missile destroyer USS Bainbridge, pictured in the foreground. While the cargo ship sailed to Mombasa with an armed guard, FBI negotiators were called in to try to secure Phillips's release. A team of Navy SEALs also arrived on scene, parachuting into the ocean with inflatable boats and were picked up by the Bainbridge. When the lifeboat ran out of gas, pirates and negotiators agreed that the Bainbridge would tow the disabled craft, and negotiators made several trips to the lifeboat in a small craft. Next
The pirate leader
Abduhl Wali-i-Musi, the pirate leader who was initially captured and let go by the crew, requested to come aboard the Bainbridge to talk and get medical attention (he was injured when the crew attacked and captured him). When he left, however, the other pirates became agitated and nervous; when one of them fired a single tracer bullet at dusk Saturday, US forces knew they had to act soon.
Wali-i-Musi is pictured here after the incident on April 20, 2009, in New York City, as federal agents led him into Federal Plaza to a court appointment. The sole pirate survivor was charged with 33 years in prison. Next
The rescue
The Navy SEAL snipers positioned on the tail of the Bainbridge finally had their chance to act. Equipped with night-vision equipment, they found themselves with a view of all three remaining pirates at once, one of whom held a gun to Phillips. Commander Frank Castellano (pictured, left, with Phillips, right) had been authorized by President Obama to use force only if Phillips's life was threatened; at this point, he gave the order, and the snipers fired three shots simultaneously, with one shot hitting each pirate in the head. "All of a sudden, I was sprayed with debris," Phillips said, and noted that he thought the pirates were firing on each other until a SEAL came into the lifeboat to ask if he was OK. He was taken to the Bainbridge, and then to Kenya, where he flew home.
The homecoming
After his flight from Kenya to Burlington International Airport in Burlington, Vt., on April 17, 2009, Phillips was greeted by (from left): his mother, Virginia, his son Daniel, his daughter Mariah, and his wife, Andrea. In addition to his family, he was met at the airport by a large crowd of media members and ordinary citizens who admired his bravery. More homecoming celebration awaited him in his home state. Next
From sea to the big screen
On May 9, 2009, Phillips and his wife met with President Obama in the Oval Office of the White House. Phillips's heroics led to many interviews and appearances, and in January 2011, he published his account of the hijacking, "A Captain's Duty: Somali Pirates, Navy SEALs, and Dangerous Days at Sea." Four years after the hijacking, he has met Tom Hanks, who plays him in the movie "Captain Phillips." He even joked with the famous actor that Hanks would have to get "better looking to play the part," as reported in the New York Daily News. Back to the beginning


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Shenzhen Hongfu Tongxin Technology Co., Ltd.
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The supplier's company premises has been checked by Alibaba.com staff to ensure onsite operations exist there. A third-party verification company has confirmed the legal status of the supplier.
|Business Type:
|Manufacturer, Trading Company
|Guangdong, China (Mainland)
|Main Products:
|FRP products,Plastic sheet,Insulation Sheet,Vaccum Forming,Roof box
|Total Employees:
|101 - 200 People
|Total Annual Revenue:
|US$10 Million - US$50 Million
|Year Established:
|Top 3 Markets:
|North America 22.00% Domestic Market 14.64% Western Europe 12.70%
Welcome to Shenzhen Hongfu Tongxin Technology Co., Ltd! Shenzhen Hongfu Tongxin Techno...
Welcome to Shenzhen Hongfu Tongxin Technology Co., Ltd!
Shenzhen Hongfu Tongxin Technology Co., Ltd is a privately owned factory focuses on the application of thermoplastic materials in engineering. Hongfu now is the leading supplier of engineering thermoplastic in stock shapes and components in China. With more than 10 years of producing experience, the company becomes a respected Manufacturer, Supplier and Exporter whose product range encompasses Insulation Material and Engineering Plastic. Product details such as POM sheets and rods, PP sheets and rods, PU sheets and rods, FR4 sheet, PE sheets and rods, Nylon (PA) sheets and rods, durostone sheet and so on.
Hongfu are committed to provide the best quality, competitive price and timely delivery. In the meantime, we ensure proper functioning of production, packaging, marketing and distribution processes.
Hongfu trades and exports all grades of Engineering Plastic Products in multiple design, shape and size that conform to the international standards. We also provide fabrication as well as moulding services; with the aim of "providing solutions for our customers". Hongfu is able to provide various solutions in accordance with our clients' requirement.
We have the largest stock of plastic Products in Shenzhen for your immediate needs. Visit our trade counter, where you will find our full range of plastic materials on display. Our sales advisors will be more than happy to provide advice on material selection and installation.
We appreciate your visit on our site, welcome to contact us!
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Trade & Market
|Main Markets
|Total Revenue(%)
|North America
|Domestic Market
|Western Europe
|Northern Europe
|Southeast Asia
|South Asia
|South America
|Eastern Asia
|Eastern Europe
|Mid East
|Central America
|Southern Europe
|Export Percentage:
|81% - 90%
|Export Mode:
|Using an agent
|No. of Employees in Trade Department:
|6-10 People
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