text:
We are glad to report about another successful pursuit in Southwest Primorye!
At noon on Friday, January 14, the Southern anti-poaching team found footprints belonging allegedly to two men and followed a trail of footprints to catch up with and arrest the intruders. After a 2-hour pursuit the rangers noticed that the violators entered Kedrovaya Pad Nature Reserve. At 4:00 p.m. the footprints belonging to one of the trespassers were lost on the southern slopes of a bare hill. Later on, a man with an illegal hunting rifle was arrested and taken into police custody. A criminal proceeding was initiated.
It is obvious that the rangers do a great job patrolling two protected areas, Leopardovy Wildlife Refuge and Kedrovaya Pad Nature Reserve, home to Amur leopards and tigers. And it is possible thanks to your generosity, our supporters, and rangers' commitment to nature conservation.
On behalf of our staff, I wish you joy and peace in 2011.
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text:
In theory, yes - flying time is only about three to four hours - but in practice it wouldn't be very relaxing. The larger islands are served by dozens of charter flights every week from May to late September, but with a charter you would need to stay for at least one week.
The alternative is to take a scheduled flight, but these are not direct: you would have to go via Athens and then switch to an internal flight on one of the Greek airlines such as Olympic or Chronos. Alternatively, you could get a return to Athens for two or three nights, head straight for the port at Piraeus by taxi or using the regular bus service, and hop on a fast hydrofoil (increasingly common in Greece) to one of the closer islands, such as Aegina, Hydra or Poros.
Breakfast at Heathrow and a late lunch on Hydra is quite feasible. The problem with the islands close to Athens is that they are usually very busy, especially at weekends, when many Athenians also take a boat out to them. Accommodation can be hard to find, especially at this time of year. The islands are for relaxing, and this isn't the best way to do that.
It is very easy to travel independently. It's also a great way to see the islands, as long as you're flexible. I recently flew to Rhodes with the intention of going to Naxos, only to find that the day of the weekly ferry had changed without warning and I missed it by half a day. There might or might not be one the following week, the ticket agent told me, and there might or might not be a boat back again. I would have to go to Naxos to find that out.
This was early in the season when boats are more likely to be changed or cancelled, but at any time of year, a Greek ferry timetable is best viewed as a work of fiction. You must travel with the frame of mind that you may not get to the island you particularly wanted to, but you will get to another one that is just as interesting.
That aside, it is easy to get around. To go to Rhodes I booked the cheapest convenient charter ticket through the Internet, using the Cheap Flights Website (www.cheapflights.co.uk). I also booked my first two nights' accommodation in the same way, at the St Nikolis Hotel in the Old Town of Rhodes, using one of the many Greek travel websites, in this case one for Rhodes itself (www.rodosnet.gr/Defaultuk.htm).
Having missed my Naxos ferry, I got one to Symi instead and moved on from there. I ended up touring the Dodecanese instead of the Cyclades, but what the heck. English is spoken everywhere, so you will have no trouble getting ferry tickets, finding rooms or ordering meals. Only in the smaller islands are you likely to find that a bit of Greek is needed, and in those places it really is a bonus. Pack the Rough Guide to Greek. It is at least worth making the effort to learn the Greek alphabet, so that you can read street signs, bus destinations and so on.
If you avoid late July and August, and Easter, you should have no trouble finding rooms, at whatever price you want to budget for. Ferries are usually met by hotel owners and householders who have rooms to rent, so just hang around looking like someone who doesn't have a room. You can trust most people, and they will usually have photos of the accommodation available. Haggling is usually expected, though not always in the hotels, whose room rates are fixed by the Tourist Police and should be on display.
Taking a package is usually cheaper if you need to be sure of the dates you travel, and only want to stay on one island, or perhaps a week each on two different ones, though the extra cost of this is enormous. A good two-island combination is Symi and Tilos, with Greek specialists Laskarina Holidays (01629 822203). A week on Symi costs from pounds 365 and a week on Tilos from pounds 395, both till mid-July and based on two people sharing. Add pounds 22 for the transfer cost, a pounds 20 admin cost, and that's pounds 802 per person. Stay in the same property on Symi for the full fortnight and the cost is only pounds 440.
You don't have to have a fixed base, though. Sunvil (0181-568 4499) will arrange an island-hopping itinerary through the Cyclades for you. Expect to pay around pounds 500- pounds 600 for one week, pounds 650-pounds 800 for a fortnight.
Easily, with a little pre-planning, and extra journey time. The busiest islands tend to be the ones with airports, such as Corfu, Rhodes and Kos. Slightly quieter are the islands one boat ride away, such as Paxos, Halki or Kalymnos respectively. If you travel two islands away from an airport, you start to find islands that are more Greek and less geared to package tourism.
In most cases you can still get there in one day from Britain, and the specialist tour operators can make it easier by chartering their own small boats that take you straight there. Both Laskarina and Sunvil (see above for contact details) offer slightly more remote islands, such as Lipsi, Leros and Lemnos - which seems to suggest that any island beginning with an `L' is OK.
Even Lesbos, the third-largest Greek island and one which has some busy resorts, also has the delightful village of Sigri, at the extreme western corner, which is too small to attract the mainstream operators. Direct Greece (0181-785 4000) currently goes there; a fortnight in early July costs from pounds 359, based on two sharing a small villa. If you want to really escape the crowds then you will either have to make your own way to one of the more distant islands or get a copy of the Hidden Greece brochure (0181-766 7868).
Where some operators promise you hidden Greece, and then list the same destinations as everyone else, Hidden Greece really goes there. It includes tiny specks of islands such as one-village Donousa in the Cyclades, Agathonisi in the Dodecanese, Fourni and Ikaria in the north-east Aegean, and even - if you believe it - the island of Dyspepsia in the Dolmades, best reached by five hours on a pedalo. Prices are very reasonable, too: pounds 386 for two weeks on Agathonisi in high season.
Greece is still one of the cheapest countries in Europe, and you can have a good meal for pounds 10 a head. A decent en suite hotel room can cost less than pounds 20 a night, out of high season. If you are on a very limited budget, you can do it for much, much less by staying in more basic hotels or renting a room in someone's house, and eating more simply.
If you are past the days of slumming it, but still have a limited budget, then look to the mass-market operators that offer Greece in their brochures. You have to be prepared to enter into package-holiday land, but if you have a family then that's probably what you want and, among the brochures, there are pleasant places to stay.
Lassi on Kefalonia features in many brochures. It is built-up, but not overwhelmingly so, and is very convenient for exploring the interesting Kefallonian capital, Argostoli. One week's b&b at the Hotel Lassi before 22 July costs pounds 449 per adult, pounds 315 for a child, in Unijet's (08705 336336) brochure.
If you consider that you could pay up to pounds 200 for a flight alone at that time of year, then the package is a good deal (see also the details for Sigri under "Can I Avoid the Crowds?").
The bigger islands tend to have the best beaches, so if that's what you're after, consider Crete, Rhodes or Kos. Skiathos has lots of decent beaches, and Santorini the spectacular black sand ones, though these can get unbearably hot in midsummer. The larger Ionian islands are all good: Corfu, Kefallonia, Lefkas and Zakynthos.
Not all resorts are equally appealing, though, so read the brochure carefully combined with a good guidebook. There are plenty of stone, pebble and even rocky areas in the islands which are described as beaches, and which regulars are used to, but which would probably disappoint a first-time visitor (see also the "Five of the Best Beaches").
You can if you choose a large island as, generally speaking, the smaller islands are not well off for historical remains or museums. An exception is Delos, one of the most important archaeological sites in Greece. The whole island is protected and visits are only allowed from neighbouring Mykonos. Crete, the largest island, also has plenty to offer, from Knossos and Phaestos to golden beaches and the Samaria Gorge.
The bigger the island, the more variety you will have, so choose somewhere like Rhodes, Lesbos or Euboea (which, despite being the second largest island, is not listed in some guidebooks as it is connected to the mainland by a bridge).
Another suggestion would be to stay on Poros, one of the Argo-Saronic islands near Athens. Not only could you take a day trip to Athens to see the Acropolis and other sights, the island is only a five-minute boat ride from the Greek mainland, and visits to Epidavros, Mycenae and Tiryns can be made from there.
You can eat well and you can eat badly in the Greek islands, but Greek cuisine is rather more limited than that of most other Mediterranean countries. Having said that, you won't find a better place to sit and eat fresh fish, simply grilled over charcoal, than at a waterfront taverna on one of the Greek islands.
Under a recent law, Greek menus are obliged to say whether the fish they serve is fresh or frozen. Popular dishes such as squid or swordfish are made available all the time by buying in frozen supplies, but you will see how fresh the fish is by going into the kitchen or by having the waiter bring the fish to your table for you to choose one. Bear in mind that the price on the menu is per kilo, so ask for a fish to be weighed and priced before you commit yourself to having it: fish is expensive.
Greek kitchens tend to do the bulk of their cooking in the morning, keeping things hot till they are served. In some places this can mean lukewarm food, but this is the way Greeks like it so you either accept it or order something freshly cooked, such as fish or souvlaki.
Vegetarians are being increasingly catered for, but dishes such as stuffed peppers or stuffed tomatoes may have meat in them, so check first.
Look for a place that serves more imaginative dishes than the usual list of moussaka, souvlaki, dolmades and stuffed tomatoes. There are such places, like my own favourite, the Mantalena in Alikanas on Zakynthos, where family recipes are beautifully cooked with the best local ingredients. As the owner, Tasos, told me one time when I commented on another restaurant's offering of a complete Greek meal, including wine, for 2500 drachmas (a fiver): "You pretend to pay them and they pretend to feed you."
Tasos also echoed one of my own feelings about eating in the Greek islands: be wary of eating at a place that employs someone to stand outside and try to persuade you to come in. "You would never do that to a Greek," Tasos told me, "so why do it to a tourist?" If a place is good, it is busy because of its reputation.
Greek wines have also been improving enormously in recent years, even winning prizes in international competitions, but you can still opt for cheep and cheerful table wines, or Greece's own resinated wine, retsina, which is an acquired taste that not everyone thinks worth acquiring (see also the "Five of the Best Restaurants").
LOVE THEM or loath them, with its ample sunshine quota and well- suited soil conditions, you'd be hard-pressed to avoid olives on a trip to Greece. Despite the fact that these small - or not so small, since some plump varieties grow up to an inch and a half in length - oily fruit are today cultivated across the globe from Mexico to the Mediterranean, they are naturally native to Greece.
Apparently the country is home to more than 137 million olive trees, most of which clamber skywards from the precious earth of small family plots. The olives' piquant taste and tonal differences depend very much on when the fruit is picked. Those that remain green have usually been plucked from the tree when still underripe whereas those that turn brown, plum or black have been left to ripen on the branch. Once ready for harvesting, the fruits are then dutifully hand-picked and stacked for collection by the canning companies that then send them on for sale in luxury deli counters abroad.
Of the many types of olive that can be bought in the country, Kalamata olives are some of the most prized. These enormous, jet-black berries are shiny and almond-shaped and are unique to the area around Kalamata in the southern Pelopponese region. Though the city which gives the variety their name may have little to place it on any usual tourist itinerary - especially since a catastrophic earth- quake shook it to bits in 1986 - the nearby beach at Finikounda and the old Venetian towns of neighbouring Koroni and Methoni are another story.
In these places you can get away from the normal vision of package-holiday Greece and spend your days ambling through historic towns, looking out across the Messinian gulf to the mountains beyond or pottering down to the sea for an afternoon dip in the surf.
En route to the sea, though, make sure that you stop off at a local shop to pick up a Greek- inspired picnic. A kilo of olives is probably more than the most spartan of stomachs could normally fit in in an afternoon, but since it will typically set you back a mere 750 drachmas (pounds 1.50) or so, there's no reason not to indulge.
Back in the UK, half a kilo of good quality Sifonios Kalamata olives (below) would cost you pounds 4.90 from the Athenian Grocery, 16a Moscow Road, London W2 (0171-229 6280). So offer the friends you've left behind a proverbial olive branch, and buy up 17 kilos of Kalamatas to flog back to them when you get home. That way you could spend the profits on a flight back to Athens and have a summer in the sun.
You can fly to the city from Heathrow or Gatwick on British Airways (0345 222111) and Virgin Atlantic (01293 747747); from Heathrow on Cronus Air (0171-580 3500) and Olympic Airways (0171-409 3400); but the lowest fare we have been able to find for travel in the next few weeks is on easyJet (0870 6 000 000) from Luton for pounds 141.90.
Rhiannon Batten
Lawrence Durrell described this as perhaps the most beautiful beach in the world, in Prospero's Cell. Writers are prone to exaggeration, of course, but it is probably the most beautiful on Corfu.
The archetypal Greek beach, a long crescent of sand, often featured in the country's promotional tourist literature.
The Ionians are top heavy with classic beaches, and here's another. A walkway leads you down to a sandy bay with a dramatic cliff behind.
With pink-tinged sand, rock pools, offshore islets you can wade out to, brilliantly clear water, a few tavernas and a fairly remote setting on the island's south-west. Idyllic.
One of the best on an island noted for its beaches, this 1,000-metre stretch of sand has a sweep of pine trees behind it that offer some shade.
A family-run place that makes its own wine, serves complimentary ouzo, and produces exemplary food.
A unique institution which seems to run on chaos, but produces fine food. In high season you'll have to queue for a table.
Combining Greek tradition with Mediterranean invention, this is one of the best restaurants in the Dodecanese, yet few tourists make it to this part of town.
Visiting dignitaries are taken here to dine. It serves some of the best food on Corfu, with many local specialities and good wines.
The elderly Vassili still oversees his family restaurant. Right on the harbour, it provides sophisticated versions of Greek classics, and fresh fish.
- More about:
- Athens
- Heathrow Airport
- Mediterranean
- Rhodes

subset: commoncrawl


text:
I don't need to go into many details about the startup process and importance of analysing it in case of problems. Here's how I do it:
The tools:
Wireshark (http://www.wireshark.org/)
Windows ResourceKit tools (http://www.microsoft.com/downloads/details.aspx?FamilyID=9d467a69-57ff-4ae7-96ee-b18c4790cffd)
Install the tools accepting all defaults (you should always go with defaults unless you have really good reasons not to – and security through obscurity is not one). Follow the Resource kit documentation to install Autoexnt service, use interactive option.
The most important information that is not in the network traffic capture is the process map – the information that allows to identify what processes are making connections.
I'm using c:\tmp folder for the captures and other files. This is the autoexnt.cmd file:
@echo off
move c:\tmp\capture.cap c:\tmp\captureX.cap
move c:\tmp\capturelog.txt c:\tmp\capturelogX.txt
start /D"C:\Program Files\Wireshark\" tshark.exe -i 2 -w c:\tmp\capture.cap
cscript //Nologo c:\tmp\now.vbs >> c:\tmp\capturelog.txt
netstat -ano >> c:\tmp\capturelog.txt
pslist >> c:\tmp\capturelog.txt
sleep 1
goto loop
In the tshark command line options, the interface number (the -i option) may be different on your system – use "tshark -D" to list interfaces on your system. I found that in some cases tshark has visibility of all interfaces on the system whereas Wireshark GUI doesn't let you choose the right interface. Now.vbs prints current time with seconds. The whole script is:
WScript.Echo Now
After rebooting the computer and the user logon there will be two windows on the screen – cmd.exe and tshark.exe. Close both -you'll find the traffic capture in the c:\tmp\capture.cap and process/connection lists in c:\tmp\capturelog.txt. That's enough information to do analysis.
The beauty of the approach is that no hubs or switches are involved, and all of it can be done remotely. Evidently, both scripts and the approach can be improved in many ways. Suggestions welcome.


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Nutrition and Canned Dog Food
15 Reasons to Consider Adding Canned Food to Your Dog's Diet
Canned dog foods have many benefits for optimal nutrition that every pet parent should consider for their beloved dog.
Canned food formulas for Dogs:
- Palatability – Some dogs prefer canned food over better dry formulas. Canned dog foods are extremely palatable making them a great choice for picky eaters.
- Decreased Stool Volume – Dogs fed canned food tend to have a lower stool volume when feeding a canned diet.
- Convenience – It's a time saver on rushed morning to just open the can for quick feeding.
- For Highly Active Dogs – Many young and active dogs require large amounts of calories in order to grow and maintain a vigorous life style. The little Jack Russell Terrier that runs two inches off the ground at all times, investigating the slightest activity, may need additional calories above the amounts in some dry formulas.
- As a Complement to Dry Food – Complete and balance canned formulas can be fed alone or as a complement to the dry diet.
- For Senior Dogs – Older dogs some times loose interest in eating and pet parents become concerned about how to stimulate their appetite. Canned foods are very palatable and will generally work well to encourage better eating enthusiasm.
- To Disguise Medications – Many pets, especially senior dogs take regular medication. Canned foods offer an easy and convenient way to disguise medications and reduce the stress of giving a pill for both the pet and the pet parent.
- For a Rotational Diet – Many dog owners like to provide a variety in their pet's diet. Rotating the different varieties of canned foods is a convenient and nutritious way to do that every day.
- Variety is the Spice of Life – Moist foods come in multiple flavors and in different textures and packages. Use a variety of flavors to keep your dog interested in his food at all times.
- As a Nutritious Reward – Many pet parents like to offer a nutritious reward for their dog as a thank you for all they give back to us each and every day. Wellness canned food formulas are a great reward for a well behaved dog.
- To Increase Water Intake – Feeding moist food is a good way to increase the daily water intake of a pet. The water content of canned foods is usually about 70 to 78 %; with pouches containing more water at 82%.
- For Thin Dogs – Moist foods contain high levels of meat and are often used to enhance the fat and protein content of dry foods without adding excess carbohydrate or fiber.
- Longer Shelf Life – Canned foods are preserved with heat sterilization and vacuumed preservation in an oxygen free environment.
- For Dry Skin & Coat – Moist foods are often fed to animals with dull dry skin and coat condition to benefit the additional protein and essential fatty acids.
- To Address Food Sensitivities – Some canned foods are designed for pets suspected of having food sensitivity issues. Our Wellness Simple canned recipes feature limited ingredients and grain free options.
Wellness canned foods are an excellent choice for addressing all of the above benefits. In addition, the diverse Wellness canned food varieties are an ideal complement to dry formulas or as a complete and balanced diet fed alone.Take the time to review the varieties of Wellness® canned diets for dogs and choose the right formula to enhance the nutrition for your cherished pet.


text:
- You are here:
- Home
- Community
- Alamo Regional Mobility Authority
- About
- RMA Board
- Mr. James "Rad" Weaver
Mr. James "Rad" Weaver
Alamo Regional Mobility Authority
Appointed by Governor Greg Abbott
James "Rad" Weaver is CEO of McCombs Partners in San Antonio, Texas, and oversees the implementation of the company's investment strategies, including management of direct investments in private operating businesses. He served as an Investment Analyst at McCombs Enterprises since March 2000.
He serves as Chair-Elect of the San Antonio Chamber of Commerce and has been a Director of the San Antonio branch of the Federal Reserve Bank of Dallas since April 2014. He is currently serving as the Presiding Officer of the Alamo Regional Mobility Authority. Mr. Weaver is also a member of the Texas Business Leadership Council and a board member of the McCombs School of Business at the University of Texas at Austin
He is currently serving on multiple corporate boards including Southern Towing Company, Circuit of the Americas, Ithaca Holdings, Allegiance Ambulance, General LED, I. Am. Plus Electronics, Mediterranean Resources, Rev Worldwide, KO holdings, and Social Money.
He holds a Bachelor's degree in Business Administration from the University of Texas at Austin.
Contact Rad Weaver


text:
Award Categories
Enterprise Mobility
First year awarded:
Number of MVP Awards:
Email: email@example.com
Sainath K E V
Marsfield, New South Wales
Sainath is a Senior Infrastructure Consultant at Avanade Australia, providing homogeneous to heterogeneous solutions to SME and large scale enterprise customers. With 13 years of industry experience, he has expertised on Active directory Design and Architecture, Microsoft Private Cloud, Identity Management, SCOM, SCVMM and Microsoft Lync, along with win32 , C++ , Device Driver programing. He works on Operating System internals and windows debugging. With both skills of Infrastructure and Software he provides excellent solutions for complex infrastructures. Sainath is a accomplished speaker for Microsoft , an author for Microsoft Technet Magazine and a reviewer for Microsoft Operations Framework (Active Directory , Certificate Services, Hyper-V and Windows Server 2008 ) . He is also Moderator for Technet forums for Windows servers and Windows Networking platforms.


text:
about us
privacy policy
send email
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> Guava
Anshu's Guavas are grown in California and hand selected for their excellent quality. This round to oval shaped subtropical Guava fruit is actually classified as a berry. The edible skin is light to strong green or yellow in color and the flesh can be creamy white, pink or red depending on the variety of Guava.
Anshu's Guavas are harvested when just ripe. Anshu's Guavas have a refreshing, mildly sweet, fruity and tart flavor. Anshu's Guavas are ripe when soft and creamy in texture. They have an edible rind and small seeds which are also edible. No one can resist their rare and irresistible scent, with its delicate floral aroma. One bite will transport you to the tropics!
Anshu's Guavas are known as "Super Fruits", being rich in vitamins A and C, Omega 3 and Omega 6 polyunsaturated fatty acids and especially high levels of dietary fiber.
Anshu's Guavas contain both carotenoids and polyphenols – the major classes of antioxidant pigments, giving them relatively high dietary antioxidant value among plant foods.
A single Apple Guava (P. guajava) fruit contains over four times the amount of vitamin C as a single orange (over 200 mg per 100 g serving) and also has good levels of the dietary minerals, potassium, magnesium, and generally a broad, low-calorie profile of essential nutrients. Wash and cut lengthwise into 6 to 10 even slices. The skin and seeds are edible.
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text:
Talk:Battlefield Earth (novel)
|Battlefield Earth (novel) was a Language and literature good articles nominee, but did not meet the good article criteria at the time. There are suggestions below for improving the article. Once these issues have been addressed, the article can be renominated. Editors may also seek a reassessment of the decision if they believe there was a mistake.
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Proposed edit[edit]
I've been doing some research and found some information that might balance out this article a bit.
Add this text: " L. Ron Hubbard wrote the novel's introduction on October 1980, and noted that Battlefield earth "may be the biggest SF novel ever in terms of length." in the lead paragraph AND "Battlefield Earth often echoes Planet of the Apes, the film Franklin Schaffner made in 1968 fro Pierre Boulle's novel, according to writer Robert Fullford" in the critical response section.
I retrieved the information from the following reference: Fulford, R. (2000, May 09). Battlefield earth: A true cult classic: Scientology founder L ron hubbard built empires in his pulp fiction, too. National Post, pp. B.1.FRO-B1 / FRONT — Preceding unsigned comment added by Orangepulp81 (talk • contribs) 22:35, 30 June 2011 (UTC)


text:
Private and Government both type of hospitals are present in Kerala. Government hospitals is less expensive than private hospitals of Kerala. The place where medical facility is small compared to hospitals are called clinics, these are also available in Kerala. Dental clinics in Kerala provide dental treatment like root canal therapy, teeth straightening and more. Many homeopathic clinics are present in Kerala, which work on natural approach. Not only in Kerala, hospitals are necessary in every place of India to make every citizen of country healthy. Some hospitals have good reviews like Al Arif Hospital, Al Shifa Hospital Private Limited.
Notify me of follow-up comments


text:
Norcross, GA
In this role, you will participate in the design, implementation and integration of all accounting for income tax work processes to meet timely and accurate reporting standards of the company. The selected candidate will monitor ongoing tax legislation and inform management of the potential impacts of the Company.
- industry
- Business Services
- experience
- Less than 5 years


text:
Donald Sterling strategy struck down in court
LOS ANGELES – One of the key legal strategies employed by Donald Sterling and his lawyers was shot down Friday in a surprise ruling.
Judge Michael Levanas said the legislature hasn't granted a judge in probate court the authority to strike medical testimony on the basis of privacy violations.
Lawyers for the banned Clippers owner had argued doctors violated Sterling's medical privacy by releasing the results of mental examinations to his wife, Shelly, and her lawyer, Pierce O'Donnell.
Both legal teams gathered inside Levanas' courtroom in downtown Los Angeles to discuss upcoming witnesses. With one full day and two afternoons scheduled for trial next week, Levanas ruled on the defense in an effort to narrow the scope of testimony.
"They lost a major defense today," O'Donnell said.
Bobby Samini, one of Donald Sterling's lawyers, is convinced Sterling's privacy rights were violated.
"That's a tough one," he said. "We feel very strongly. That's a core, privacy rights issue. The message it sends to all of America is your medical records can be released, and once the damage is done, the court won't exclude it.
"I don't agree with that decision. I think that goes way beyond this case."
In the Sterling Family Trust documents, Donald Sterling agreed to waive his HIPAA rights, but his lawyers had challenged the validity of that waiver.
The trial is set to resume Monday morning, with Donald Sterling's team putting on a list of witnesses that won't include O'Donnell, the man they've argued masterminded Sterling's removal from the family trust.
Levanas ruled O'Donnell would not be allowed to testify and provide evidence against his own client.
Still, Samini said there's "one very important decision the judge is reserving to the end."
Samini said the issue of revocation, which has still not been decided, could play a crucial role in delaying the sale of the Clippers to Steve Ballmer, though his attorney disagrees.
"That is going to be a nothing issue," Adam Streisand said.
Streisand said he and Shelly Sterling's legal team also could've challenged whether Donald Sterling had mental capacity to revoke the trust, but they wanted to avoid a "battle of the experts over his capacity."
"It would've just lengthened the trial," he said.
And that, Shelly Sterling's lawyers said, is Donald Sterling's lone remaining goal — to force the NBA to seize the Clippers on Sept. 15 instead of allowing the $2 billion sale to Ballmer to continue.
"It's a suicide mission by a mad man," Streisand said.
Contact the writer: email@example.com
- Silver: NBA didn't get proposal from Donald Sterling
- Donald Sterling lashes out at wide array of targets in testimony
- Back on the stand, Sterling calls wife a 'pig,' rips NBA
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Buttercream rose swirl cake
And another buttercream cake! This time topped with the rose swirls that I normally use to top cupcakes.
This cake was more difficult to do as you're covering edges with round swirls but I like the effect and think it gives another different look to choose from.
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For more than 100 years the Dorotheum has been holding its own specialist auctions of autographs. In the twice yearly auctions, collectors can find autographs of well-known figures from the widest variety of genres, such as history, science, philosophy, music, the visual arts, architecture, literature, theatre and film. Collectors also purchase historic letters and documents, scrolls and manuscripts. Some of the highlights of our more recent auctions: letters of Ludwig van Beethoven, Anton Bruckner, Richard Wagner, Johannes Kepler, Friedrich von Schiller, Empress Elisabeth of Austria and Romy Schneider. With the sale of the Le Puy-en-Velay codex for 356,800 euros the autographs department achieved an especially remarkable success.
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Urban Vintage || August 12 & 13 2016
Urban Vintage is a two day shopping event, August 12th & August 13th at Traine in Seaboard Station This is a BRAND NEW VENUE for us so come and help us celebrate!
If you like antique, vintage, retro, mid-century, salvage and industrial goods, we will have the very best 'pickers' showing what they've found!
The industrial, vintage movement has crept into almost every scenario. Shop owners need new display ideas. Pinterest lovers want more choices. We are talking about a top notch, drool worthy, INDOOR, rusty junk market with an urban flair. This is going to be a two day event in and around Seaboard Station. After you shop with us, check out the fantastic local shopping and dining spots Seaboard Station has to offer!
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CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls
DOI: 10.1186/ar1175
© Li et al.; licensee BioMed Central Ltd. 2004
Received: 30 December 2003
Accepted: 15 March 2004
Published: 27 April 2004
Bacterial infections play an important role in the multifactorial etiology of rheumatoid arthritis. The arthropathic properties of Gram-positive bacteria have been associated with peptidoglycan–polysaccharide complexes (PG-PS), which are major structural components of bacterial cell walls. There is little agreement as to the identity of cellular receptors that mediate innate immune responses to PG-PS. A glycosylphosphatidylinositol-linked cell surface protein, CD14, the lipopolysaccharide receptor, has been proposed as a PG-PS receptor, but contradictory data have been reported. Here, we examined the inflammatory and pathogenic responses to PG-PS in CD14 knockout mice in order to examine the role for CD14 in PG-PS-induced signaling. We found that PG-PS-induced responses in vitro, including transient increase in intracellular calcium, activation of nuclear factor-κB, and secretion of the cytokines tumor necrosis factor-α and interleukin-6, were all strongly inhibited in CD14 knockout macrophages. In vivo, the incidence and severity of PG-PS induced acute polyarthritis were significantly reduced in CD14 knockout mice as compared with their wild-type counterparts. Consistent with these findings, CD14 knockout mice had significantly inhibited inflammatory cell infiltration and synovial hyperplasia, and reduced expression of inflammatory cytokines in PG-PS arthritic joints. These results support an essential role for CD14 in the innate immune responses to PG-PS and indicate an important role for CD14 in PG-PS induced arthropathy.
Keywordsbacterial cell surface molecules rheumatoid arthritis transcription factors
Rheumatoid arthritis (RA) is characterized by a chronic, erosive inflammation of peripheral joints. The etiology of RA is not known, but both genetic and environmental factors contribute to the disease. Gram-positive bacterial infection is one environmental factor that has been linked with RA pathology [1]. The link between systemic bacterial infection and RA is based on observations that bowel-related diseases such as Crohn's disease and ulcerative colitis are frequently complicated by arthritis, that RA patients have significantly elevated levels of antibodies to bacterial products (e.g. antipeptidoglycan antibodies), and that bacterial products can frequently be found in RA synovial fluids and tissues [2–5]. The arthropathogenic properties have been attributed to poorly degradable peptidoglycan–polysaccharide complexes (PG-PS) – covalently bound polymers that comprise cell walls of Gram-positive bacteria [2, 6]. When injected in susceptible strains of animals, crude preparations of bacterial cell walls or purified PG-PS produce disease with clinical features resembling those of human RA [6].
Injected PG-PS accumulate in macrophages of the spleen, liver, and mesenteric lymph nodes [7], resulting in persistent cell activation and secretion of inflammatory cytokines. The activation of macrophages by PG-PS represents an example of innate immune response, a defensive reaction based on recognition of conserved pathogen-associated molecular patterns (PAMPs) shared by large groups of micro-organisms (for review [8]). PAMP recognition in mammals is mediated by the Toll-like receptor (TLR) family of pattern recognition receptors. Engagement of TLRs triggers signal transduction pathways that culminate in the activation of transcription of defensive genes. Individual TLRs produce overlapping but distinct patterns of gene expression, permitting tailoring of host responses to different pathogens. The precise molecular mechanisms whereby distinct PAMPs activate individual TLRs are not well defined. Recognition of lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria, is the best studied model of innate immunity [9]. In serum, LPS molecules are opsonized by LPS-binding proteins that recruit LPS to CD14 – a glycosylphosphatidylinositol-anchored cell surface protein that is expressed by phagocytic cells. The tertiary LPS–LPS binding protein–CD14 complex generates the transmembrane signal of cell activation through an association with the TLR-4 receptor. A principal target and mediator of the TLR-4-induced signaling is the transcription factor nuclear factor-κB (NF-κB), which controls the expression of a variety of proinflammatory cytokines, including IL-1, IL-6, and tumor necrosis factor (TNF)-α [8, 10].
It is less clear which primary events and cellular receptors mediate the innate immune responses to and the arthritopathogenic properties of PG-PS. The intracellular signaling that occurs in response to stimulation with peptidoglycans of Gram-positive bacteria is triggered by TLR-2 [11]. A number of in vitro studies have suggested that, similar to the involvement of CD14 in LPS signaling, CD14 plays an essential role in the activation of PG-PS induced innate immune responses [12–16]. However, there are reports that peptidoglycans can directly bind TLR-2 [17] and activate cells independently of CD14 [18]. Studies conducted in vivo have also yielded contradictory results. Whereas CD14 knockout mice were resistant to Gram-negative bacteria-induced and LPS-induced shock [19], CD14-deficient mice, when challenged with live or killed Gram-positive bacteria, had survival rates similar to those in wild-type animals [20].
In order to elucidate the role played by CD14 in the innate immune response to PG-PS in vitro and in vivo, we assessed the inflammatory responses to PG-PS in CD14 knockout primary macrophages and examined the development of PG-PS induced arthritis in CD14 knockout mice. Our findings demonstrate that, in CD14 knockout macrophages, PG-PS failed to activate inflammatory responses in vitro, and that the development of PG-PS induced arthropathy was significantly inhibited, albeit not abolished, in CD14 knockout mice.
Materials and methods
Purified PG-PS (fraction 100P) from group A Streptococcus pyogenes was purchased from Lee Labs (Garrison, GA, USA). Endotoxin contamination was under 0.35 pg endotoxin/μg PG-PS, as determined using a Limulus amebocyte lysate assay system (Pyrogent®plus; Biowhittaker, Walkersville, MD, USA). Therefore, the concentration of endotoxin in in vitro experiments was under 35 pg/ml, even at the highest (100 μg/ml) concentrations of PG-PS. All reagents, unless otherwise noted, were from Sigma (St. Louis, MO, USA) and the culture medium was from GIBCO (Grand Island, NY, USA).
Animals and arthritis model
Female CD14 knockout mice [19] were backcrossed 10 times onto the BALB/c background, as described previously [20, 21]. The expression of CD14 mRNA was evaluated by using RNase protection assay, as described previously [21]. Age-matched wild-type female BALB/c mice were used as controls. To induce acute polyarthritis, animals were injected intravenously with PG-PS (3 mg rhamnose/kg). At this dose, the maximal amount of co-injected contaminating endotoxin was less than 30 pg/mouse. The development of arthritis was evaluated by using a scoring system based on a scale from 0 to 4 for each of four paws, as described elsewhere [22]: 0 = normal paw; 1 = swelling of individual digits; 2 = moderate swelling and redness of ankle or wrist joints; 3 = swelling and redness of at least two joints; and 4 = swelling of the whole paw. According to this system, the total arthritis score of a mouse varied in the range 0–16. To compare the frequencies of arthritis in wild-type and CD14 knockout animals, each mouse that had any signs of arthritis (i.e. total score ≥ 1) was considered positive. To minimize bias, animals were coded and assessed randomly.
Histologic analysis
Following the intravenous PG-PS injection on day 0, mice were killed on the next day after the joint inflammation had reached a peak (usually, on day 4). The hind ankle joint and paws were fixed in 10% formalin, decalcified, paraffinized, cut into 5 μm sections, deparaffinized, stained with hematoxylin–eosin, and evaluated histologically. Morphologic assessments of arthritis were performed in a blinded manner using a scoring system with a scale from 0 (no damage) to 4 (severe damage), based on the overall infiltration of inflammatory cells, synovial hyperplasia, and deposition of fibrinous exudates in the joints and surrounding tissue [4].
Cell culture
Splenocytes were harvested as described elsewhere [23]. Cells were resuspended in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, HEPES (10 mmol/l) and antibiotics (100 U/ml penicillin G and 100 μg/ml streptomycin sulfate), and incubated at 37°C in 5% carbon dioxide for 1 hour. The nonadherent cells were washed out gently twice with phosphate-buffered saline and once with DMEM. Cell viability was typically in excess of 95%, as assessed by trypan blue exclusion. Peritoneal macrophages were collected as previously described [20]. Cells were recruited by an intraperitoneal injection of a sterile solution of 3% thioglycollate. Three days after intraperitoneal injection, the peritoneal exudates were collected and washed thee times, and cells were allowed to adhere to plastic for 30 min, followed by removal of nonadherent cells. Unless otherwise indicated, cells were maintained for 24 hours before use in a DMEM media at 37°C in 5% carbon dioxide.
Assessments of intracellular calcium
The intracellular calcium concentration ([Ca2+]i) in macrophages was measured using fura-2, a fluorescent [Ca2+]i indicator, as described elsewhere [23]. Macrophages were plated on coverslips in a DMEM media supplemented with 10% fetal bovine serum at 24 hours before measurement. Growth media was replaced with a modified Hanks' balanced buffer (115 mmol/l NaCl, 5 mmol/l KCl, 0.3 mmol/l Na2HPO4, 0.4 mmol/l KH2PO4, 5.6 mmol/l glucose, 0.8 mmol/l MgSO4, 1.26 mmol/l CaCl2, and 15 mmol/l HEPES; pH = 7.4) containing 5 μmol/l acetoxymethyl ester of fura-2 (Molecular Probes, Eugene, OR, USA). Cells were incubated at room temperature for 30 min and stimulated with indicated concentrations of PG-PS. The [Ca2+]i measurements were performed in individual cells by using a microspectrofluorometer (InCyt Im2™ Imaging, Cincinnati, OH, USA) interfaced with an inverted microscope TMS-F (Nikon, Kanagawa, Japan) [23]. The fluorescence intensity was monitored at excitation wavelengths 340 and 380 nm, and emission wavelength 510 nm. Each value was corrected by subtracting the system dark noise and autofluorescence. The [Ca2+]i was calculated as [Ca2+]i = Kd([R - Rmin]/[Rmax - R]) × (F0/Fs) as previously described [23]. Cells were selected at random and analyzed using InCyt Im2™ image acquisition and analysis software.
Assessments of nuclear factor-κB activation
Cellular localization of the RelA (p65) subunit of NF-κB was detected by immunocytochemistry. Splenic macrophages were stimulated with PG-PS for 30 min, fixed for 10 min with ice cold methanol, and blocked for 30 min in a 10% normal goat serum (Sigma). Cells were immunostained using a primary polyclonal rabbit anti-RelA antibody (Rockland Immunochemicals, Gilbertsville, PA, USA) diluted at 1:200 in 10% normal goat serum, and visualized with the secondary rhodamine isothiocyanate-conjugated antirabbit antibody. Nuclei were counterstained with Hoechst 33342 (Molecular Probes).
RNA isolation and reverse transcription polymerase chain reaction
Total RNA was extracted from homogenized tissues by using Trizol reagent (Sigma). One nanogram of RNA was subjected to reverse transcription by using MLV reverse transcriptase (Promega, Madison, WI, USA). The resulting cDNA was PCR amplified with the following primers: TNF-α: 5'-TAC TGA ACT TCG GGG TGA TTG GTC C-3' (forward) and 5'-CAG CCT TGT CCC TTG AAG AGA ACC-3' (reverse); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH): 5'-TGA AGG TCG GTG TCA ACG GAT TTG-3' (forward) and 5'-GTA CAT CCG TAC TCC AGG TGG TG-3' (reverse). In a typical PCR reaction, 50 ng cDNA template was added to a mixture containing 75 μmol/l of primers, 2.5 mmol/l dNTPs, and 1 U Taq polymerase. For detection of TNF-α message, the template was amplified by 26 cycles of PCR (at the annealing temperature 56°C). GAPDH was amplified by using 24 cycles (annealing temperature 63°C). The PCR products were visualized by agarose gel electrophoresis.
Statistical analysis
Unless otherwise indicated, all results are expressed as means ± standard error of the mean. Statistical differences between groups were determined using analysis of variance with Tukey's or Scheffe's post hoc test. Scores were compared using the Mann–Whitney rank sum test. P < 0.05 was considered statistically significant.
CD14 mediates the PG-PS-induced increase in intracellular calcium concentration
PG-PS-induced nuclear factor-κB activation is mediated by CD14
The transcription factor NF-κB is a pivotal regulator of genes that are involved in inflammation and immunity [26]. NF-κB is an essential component of TLR signaling [8]. The activity of NF-κB is controlled by interaction an inhibitory molecule known as IκB. Cell stimulation induces degradation of IκB, thereby allowing NF-κB to enter the nucleus and initiate the transcription of target genes [27]. Thus, the nuclear localization of NF-κB is indicative of NF-κB activation.
CD14 mediates PG-PS-induced production of inflammatory cytokines
The severity of PG-PS-induced arthritis is attenuated in CD14 knockout mice
The etiologic agents of RA remain to be identified. However, in spite of the inability to isolate a specific infectious organism from the joints of RA patients, there is evidence suggesting that transient exposure to indigenous bacterial products may provoke an initial response that could eventually perpetuate and amplify itself. This notion has been supported by numerous animal studies. Single intraperitoneal injection of crude bacterial cell walls or water-soluble PG-PS from Gram-positive bacteria into susceptible strains of rats induces polyarthritis that closely resembles human RA. The joint lesions have a biphasic course with an initial acute inflammation of the ankle, wrist, and small joints of extremities, followed by chronic erosive arthritis (for review [6]). The initial, acute, phase has features of the innate immune response in that inflammation is driven by neutrophils and macrophages and is T-cell independent. The chronic stage of arthritis is T-cell dependent and thus has features of the adaptive immune response [7, 29]. Systemic administration of Gram-positive bacterial cell walls or PG-PS in susceptible strains of mice also produces arthritis, although, for unknown reasons, mice develop only the acute phase [22, 30]. The minimal essential arthritogenic structures have been identified as PG-PS, the major structural components of Gram-positive bacterial cell walls [31]. The peptidoglycan moiety is responsible for the pathogenicity of PG-PS, because enzymatic digestion of the peptidoglycan moiety eliminates the proinflammatory and arthritopathogenic properties of PG-PS [32]. The peptidoglycan can substitute for PG-PS in the induction of the acute [22] but not chronic phase of arthritis [2, 33]; it has been proposed that the polysaccharide moiety protects the peptidoglycan moiety from degradation in vivo, thereby facilitating chronic persistence of PG-PS in the host [2].
The primary events and the identity of cellular receptors that mediate the innate immune response to and the arthritogenic properties of peptidoglycan and PG-PS are incompletely characterized. There is substantial evidence that the transmembrane signal leading to cell activation in response to stimulation with Gram-positive peptidoglycans is triggered by TLR-2 [11]. CD14, has been proposed as an intermediate, connecting peptidoglycan with TLR-2-induced cell activation, but the role of CD14 is controversial. On the one hand, neutralizing antibody to CD14 prevented peptidoglycan-induced cell activation, and transfection of CD14-negative cells with exogenous CD14 conferred responsiveness to peptidoglycan [14], suggesting that CD14 plays an essential role. On the other hand, peptidoglycan was shown to bind TLR-2 directly [17] and to induce the activation of TLR-2-transfected cells regardless of CD14, although CD14 increased the affinity of PG–TLR-2 interactions [17] and potentiated cell activation [18], suggesting a facultative role for CD14. It is possible that the discrepancies between the studies can be attributed to differences in experimental conditions. In this regard, using primary cells with genetically inactivated CD14 allowed for clearer interpretation. We demonstrate that, in CD14 knockout macrophages, each step of PG-PS induced signal transduction, including the transient increase in [Ca2+]i, nuclear translocation of NF-κB, and secretion of TNF-α and IL-6, were almost completely suppressed. Thus, our data strongly support an essential role for CD14 in the innate immune responses to PG-PS.
To examine the role for CD14 in PG-PS induced arthropathy, CD14 knockout mice were backcrossed to a susceptible BALB/c genetic background [22]. Because mice do not develop chronic arthritis, we were restricted to examination of acute arthritis, which is driven by the innate immune response to PG-PS. The CD14 knockout mice developed arthritis significantly less frequently (68% versus 100% in wild-type group), and the severity of arthritis was significantly reduced (average arthritis score of 2.5 versus 4.8 in the wild-type group). The gross observation data largely correlated with morphologic assessments, the most pronounced difference being in the degree of infiltration of inflammatory cells. Because CD14 knockout macrophages were largely unresponsive to PG-PS stimulation, these in vivo results were not unexpected. It was rather surprising to find that PG-PS was able to induce arthritis in a significant proportion of CD14 knockout mice. The reason for that is not clear. Our analysis of mRNA expression has shown that inflammatory gene expression in arthritic joints of CD14 knockout mice was strongly inhibited but not abolished (Fig. 6). It is possible that resident nonphagocytic cells within joints can be activated by PG-PS via CD14-independent mechanisms. In this regard, Kyburz and coworkers [34] showed that stimulation of synovial fibroblasts with staphylococcal peptidoglycan caused cell activation and elevated expression of matrix metalloproteinases and cytokines IL-6 and IL-8; it appeared that cell activation was mediated by both TLR-2-dependent and -independent pathways. It is not known whether CD14 is the major receptor for PG-PS in each cell type; alternative receptors have been proposed, including the peptidoglycan recognition proteins and nucleotide-binding oligomerization proteins (NODs) [35–37]. Nonetheless, our data indicate that CD14 is an essential receptor for activation of the innate immune response in macrophages by the arthritiogenic PG-PS, and that CD14-dependent mechanisms significantly contribute to PG-PS-induced arthropathy.
Bacterial infection has frequently been associated with RA pathology. The arthropathic properties have been attributed to PG-PS, which are major structural components of bacterial cell walls. The identity of receptors that mediate the arthropathic properties of PG-PS is controversial. Here, we examined the role played by CD14 in PG-PS-induced arthritis in mice. To do so, we used CD14-deficient knockout mice. We found that CD14 knockout macrophages were almost completely deficient in inflammatory responses to PG-PS stimulation in vitro. In vivo, the incidence and severity of PG-PS-induced polyarthritis in CD14 knockout mice were significantly reduced but not abolished, as compared with their wild-type counterparts. These results support an essential role for CD14 in the innate immune responses to PG-PS and indicate an important role for CD14 in PG-PS-induced arthropathy.
- [Ca2+]i:
intracellular calcium concentration
Dulbecco's modified Eagle's medium
glyceraldehyde-3-phosphate dehydrogenase
- NF-κB:
nuclear factor-κB
pathogen-associated molecular patterns
peptidoglycan–polysaccharide complexes
rheumatoid arthritis
reverse transcription polymerase chain reaction
Toll-like receptor
tumor necrosis factor.
The authors gratefully acknowledge the technical expertise of Mrs Julie V Mitchell and Charlotte Walters at the Center for Gastrointestinal Biology and Disease (P30 KD34987). This work was supported by NIH public health grants AR/AI-44564, 5-P60 AR-30701-14, and AR/AI-44030. SM is the recipient of an Investigator Award from The Arthritis Foundation.
Authors' Affiliations
- Firestein GS: Evolving concepts of rheumatoid arthritis. Nature. 2003, 423: 356-361. 10.1038/nature01661.View ArticlePubMedGoogle Scholar
- Schwab JH: Phlogistic properties of peptidoglycan-polysaccharide polymers from cell walls of pathogenic and normal-flora bacteria which colonize humans. Infect Immun. 1993, 61: 4535-4539.PubMed CentralPubMedGoogle Scholar
- van der Heijden IM, Wilbrink B, Tchetverikov I, Schouls LM, Hazenberg MP, Breedveld FC, Tak PP: Presence of bacterial DNA and bacterial peptidoglycans in joints of patients with rheumatioid arthritis and other arthritides. Arthritis Rheum. 2000, 43: 593-598. 10.1002/1529-0131(200003)43:3<593::AID-ANR16>3.0.CO;2-1.View ArticlePubMedGoogle Scholar
- Lichtman SN, Wang J, Sartor RB, Zhang C, Bender D, Dalldorf FG, Schwab JH: Reactivation of arthritis induced by small bowel bacterial overgrowth in rats: role of cytokines, bacteria, and bacterial polymers. Infect Immun. 1995, 63: 2295-2301.PubMed CentralPubMedGoogle Scholar
- Gravallese EM, Kantrowitz FG: Arthritic manifestations of inflammatory bowel disease. Am J Gastroenterol. 1988, 83: 703-709.PubMedGoogle Scholar
- Schwab JH: Bacterial cell-wall induced arthritis: models of chronic recurrent polyarthritis and reactivation of monoarticular arthritis. In Mechanisms and Models in Rheumatoid Arthritis. Edited by: Henderson B, Edwards JCW, Pettipher ER. 1995, London: Academic Press, 431-446.View ArticleGoogle Scholar
- Allen JB, Malone DG, Wahl SM, Calandra GB, Wilder RL: Role of the thymus in streptococcal cell wall-induced arthritis and hepatic granuloma-formation: comparative studies of pathology and cell-wall distribution in athymic and euthymic rats. J Clin Invest. 1985, 76: 1042-1056.PubMed CentralView ArticlePubMedGoogle Scholar
- Janeway CA, Medzhitov R: Innate immune recognition. Annu Rev Immunol. 2002, 20: 197-216. 10.1146/annurev.immunol.20.083001.084359.View ArticlePubMedGoogle Scholar
- Beutler B: TLR4 as the mammalian endotoxin sensor. Curr Top Microbiol Immunol. 2002, 270: 109-120.PubMedGoogle Scholar
- Zhang G, Ghosh S: Toll-like receptor-mediated NF-kappaB activation: a phylogenetically conserved paradigm in innate immunity. J Clin Invest. 2001, 107: 13-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Takeuchi O, Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa T, Takeda K, Akira S: Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components. Immunity. 1999, 11: 443-451. 10.1016/S1074-7613(00)80119-3.View ArticlePubMedGoogle Scholar
- Pugin J, Heumann D, Tomasz A, Kravchenko VV, Akamatsu Y, Nishijima M, Glauser MP, Tobias PS, Ulevitch RJ: Cd14 is a pattern-recognition receptor. Immunity. 1994, 1: 509-516. 10.1016/1074-7613(94)90093-0.View ArticlePubMedGoogle Scholar
- Dziarski R, Tapping RI, Tobias PS: Binding of bacterial peptidoglycan to CD14. J Biol Chem. 1998, 273: 8680-8690. 10.1074/jbc.273.15.8680.View ArticlePubMedGoogle Scholar
- Gupta D, Kirkland TN, Viriyakosol S, Dziarski R: CD14 is a cell-activating receptor for bacterial peptidoglycan. J Biol Chem. 1996, 271: 23310-23316. 10.1074/jbc.271.38.23310.View ArticlePubMedGoogle Scholar
- Rietschel ET, Schletter J, Weidemann B, El-Samalouti V, Mattern T, Zahringer U, Seydel U, Brade H, Flad H-D, Kusumoto S, Gupta D, Dziarski R, Ulmer AJ: Lipopolysaccharide and peptidoglycan: CD14-dependent bacterial inducers of inflammation. Microb Drug Resist. 1998, 4: 37-44.View ArticlePubMedGoogle Scholar
- Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999, 274: 14012-14020. 10.1074/jbc.274.20.14012.View ArticlePubMedGoogle Scholar
- Iwaki D, Mitsuzawa H, Murakami S, Sano H, Konishi M, Akino T, Kuroki Y: The extracellular toll-like receptor 2 domain directly binds peptidoglycan derived from Staphylococcus aureus. J Biol Chem. 2002, 277: 24315-24320. 10.1074/jbc.M107057200.View ArticlePubMedGoogle Scholar
- Schwandner R, Dziarski R, Wesche H, Rothe M, Kirschning CJ: Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2. J Biol Chem. 1999, 274: 17406-17409. 10.1074/jbc.274.25.17406.View ArticlePubMedGoogle Scholar
- Haziot A, Ferrero E, Kontgen F, Hijiya N, Yamamoto S, Silver J, Stewart CL, Goyert SM: Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity. 1996, 4: 407-414. 10.1016/S1074-7613(00)80254-X.View ArticlePubMedGoogle Scholar
- Haziot A, Hijiya N, Schultz K, Zhang F, Gangloff SC, Goyert SM: CD14 plays no major role in shock induced by Staphylococcus aureus but down-regulates TNF-α production. J Immunol. 1999, 162: 4801-4805.PubMedGoogle Scholar
- Yin M, Bradford BU, Wheeler MD, Uesugi T, Froh M, Goyert SM, Thurman RG: Reduced early alcohol-induced liver injury in CD14-deficient mice. J Immunol. 2001, 166: 4737-4742.View ArticlePubMedGoogle Scholar
- Koga T, Kakimoto K, Hirofuji T, Kotani S, Ohkuni H, Watanabe K, Okada N, Okada H, Sumiyoshi A, Saisho K: Acute joint inflammation in mice after systemic injection of the cell wall, its peptidoglycan, and chemically defined peptidoglycan subunits from various bacteria. Infect Immun. 1985, 50: 27-34.PubMed CentralPubMedGoogle Scholar
- Li X, Bradford BU, Wheeler MD, Stimpson SA, Pink MH, Brodie AT, Schwab JH, Thurman RG: Dietary glycine prevents peptidoglycan polysaccharide-induced reactive arthritis in the rat: role for glycine-gated chloride channel. Infect Immun. 2001, 69: 5883-5891. 10.1128/IAI.69.9.5883-5891.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Watanabe N, Suzuki J, Kobayashi Y: Role of calcium in tumor necrosis factor-α produced by activated macrophages. J Biochem. 1996, 120: 1190-1195.View ArticlePubMedGoogle Scholar
- Ikejima K, Enomoto N, Seabra V, Ikejima A, Brenner DA, Thurman RG: Pronase destroys the lipopolysaccharide receptor CD14 on Kupffer cells. Am J Physiol. 1999, 276: G591-598.PubMedGoogle Scholar
- Barnes PJ, Karin M: Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 1997, 336: 1066-1071. 10.1056/NEJM199704103361506.View ArticlePubMedGoogle Scholar
- Baldwin AS: The NF-κB and IκB proteins: new discoveries and insights. Annu Rev Immunol. 1996, 14: 649-681. 10.1146/annurev.immunol.14.1.649.View ArticlePubMedGoogle Scholar
- Makarov SS, Johnston WN, Olsen JC, Watson JM, Mondal K, Rinehart C, Haskill JS: NF-kappa B as a target for anti-inflammatory gene therapy: suppression of inflammatory responses in monocytic and stromal cells by stable gene transfer of I kappa B alpha cDNA. Gene Ther. 1997, 4: 846-852. 10.1038/sj.gt.3300461.View ArticlePubMedGoogle Scholar
- Schrier DJ, Schimmer RC, Flory CM, Tung DKL, Ward PA: Role of chemokines and cytokines in a reactivation model of arthritis in rats induced by injection with Streptococcal cell walls. J Leukocyte Biol. 1998, 63: 359-363.PubMedGoogle Scholar
- Onta T, Sashida M, Noriyuki F, Sugawara S, Rikiishi H, Kumagai K: Induction of acute arthritis in mice by peptidoglycan derived from Gram-positive bacteria and its possible role in cytokine producion. Microbiol Immunol. 1993, 37: 573-582.View ArticlePubMedGoogle Scholar
- Schwab JH, Brown RR, Anderle AK, Schlievert PM: Superantigen can reactivate bacterial cell wall-induced arthritis. J Immunol. 1993, 150: 4151-4159.PubMedGoogle Scholar
- Janusz MJ, Esser RE, Schwab JH: In vivo degradation of bacterial cell wall by the muralytic enzyme mutanolysin. Infect Immun. 1986, 52: 459-467.PubMed CentralPubMedGoogle Scholar
- Fox A, Brown RR, Anderle SK, Chetty C, Cromartie WJ, Gooder H, Schwab JH: Arthropathic properties related to the molecular weight of peptidoglycan-polysaccharide polymers of streptococcal cell walls. Infect Immun. 1982, 35: 1003-1010.PubMed CentralPubMedGoogle Scholar
- Kyburz D, Rethage J, Seibl R, Lauener R, Gay RE, Carson DA, Gay S: Bacterial peptidoglycans but not CpG oligodeoxynucleotides activate synovial fibroblasts by toll-like receptor signaling. Arthritis Rheum. 2003, 48: 642-650. 10.1002/art.10848.View ArticlePubMedGoogle Scholar
- Girardin SE, Boneca IG, Carneiro LA, Antignac A, Jehanno M, Viala J, Tedin K, Taha MK, Labigne A, Zahringer U, Coyle AJ, DiStefano PS, Bertin J, Sansonetti PJ, Philpott DJ: Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science. 2003, 300: 1584-1587. 10.1126/science.1084677.View ArticlePubMedGoogle Scholar
- Dziarski R: Peptidoglycan recognition proteins (PGRPs). Mol Immunol. 2004, 40: 877-886. 10.1016/j.molimm.2003.10.011.View ArticlePubMedGoogle Scholar
- Girardin SE, Hugot JP, Sansonetti PJ: Lessons from Nod2 studies: towards a link between Crohn's disease and bacterial sensing. Trends Immunol. 2003, 24: 652-658. 10.1016/j.it.2003.10.007.View ArticlePubMedGoogle Scholar
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Miniature Kolacky
|Cake compressed yeast
|5⁄8 Ounce (1 Cake, 5/8 Or 3/5 Ounce)
|1 Cup (16 tbs), scalded and cooled
|1⁄2 Teaspoon
|Sifted all purpose flour
|3 Cup (48 tbs)
|1⁄8 Teaspoon
|1 Cup (16 tbs), chilled
|Egg yolks
|1⁄4 Cup (4 tbs)
|Grated lemon peel
|2 Teaspoon
|California filling/Splendent filling
|1 Cup (16 tbs)
Soften yeast in lukewarm cream.
Stir in 1/2 tea spoon sugar and let stand 15 minutes.
Blend flour and salt.
Cut in the butter with a pastry blender or two knives until particles are the size of rice kernels; set aside.
Beat egg yolks and 1/4 cup sugar together until very thick.
Beat in cream mixture and lemon peel.
Make a well in the center of the flour-butter mixture; add egg yolk mixture and blend well.
Chill dough overnight.
Put half of the chilled dough on a lightly floured surface; roll 1/4 inch thick.
Cut out rounds with a lightly floured 1 1/2 inch cookie cutter; transfer to ungreased cookie sheets.
Make a slight depression in the center of each round and fill with about 1 teaspoonful of filling.
Repeat, using remaining dough.
Cover and allow to stand in a warm place 10 to 15 minutes.
Bake at 350°F 15 to 20 minutes, or until lightly browned.
Remove cookies to wire racks.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 4486 Calories from Fat 1873
% Daily Value*
Total Fat 211 g324.4%
Saturated Fat 130.1 g650.6%
Trans Fat 0 g
Cholesterol 1240.5 mg413.5%
Sodium 847.5 mg35.3%
Total Carbohydrates 588 g196.1%
Dietary Fiber 15 g60.1%
Sugars 169.3 g
Protein 60 g120.5%
Vitamin A 139.2% Vitamin C 28.3%
Calcium 70.6% Iron 113.6%
*Based on a 2000 Calorie diet


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On My Work with the Pioneer of Kinetic Electric Light Art, Zdeněk Pešánek (1896-1965): A Memoire
Jaromír Fiala
Vol. 13, No. 3 (Summer, 1980), pp. 182-185
Published by: The MIT Press
Stable URL: http://www.jstor.org/stable/1577814
Page Count: 4
Topics: Kinetics, Lamps, Cubism, Sculpture, Surrealism, Electricity, Visual arts, Sculptors, Transformers, Architectural design
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The author describes his experiences while working between 1933 and 1940 with the Czech sculptor, architect and kinetic artist Zdeněk Pešánek. He provides background information on Pešánek's life as an artist and architect in Prague after the end of World War I, on his work with kinetic electric light art and on the projects the author helped Pešánek to execute. The author recognizes the pioneering efforts of Pešánek in the domains of his special interest and is of the opinion that Pešánek made an error by attempting to mold kinetic art to the prevailing 'isms' of Cubism, Constructivism and Surrealism instead of recognizing that kinetic art requires an entirely different approach from that required for the traditional media of static painting and sculpture.
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Patients with aneurysms survived longer following minimally invasive endovascular aortic repair as opposed to open surgical repair, according to findings reported at an endovascular therapy meeting. The minimally invasive procedure was linked with a 30-day aneurysm-related survival rate that was more than double that of open surgery and also was associated with a reduced need for transfusions.
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It has been below freezing every night here for the past 3 days and
forecast is for at least another 3 nights. Tonight it is supposed to
get to 18, setting a new record for the date. Unfortunately, the
previous warm weather fooled many plants into breaking dormancy. Many
woodies have new leaves that will probably be toast. I have a couple
of young shrubs planted last fall that may not be well enough
established to re-leaf. Depressing.
Cathy, west central IL, z5b
On Apr 5, 2007, at 9:20 PM, Pam Evans wrote:
> What is w/ this weather? I've been wearing shorts every evening
> this week
> until tonight and now I look at the forecast and we are supposed to
> get rain
> and snow showers Saturday night?? In April? Snow showers? What the
> heck? I checked the weather for Claremore and it says it's 33
> degrees
> there now? It's in the 50's here but fixin' to drop tonight. I
> don't get
> this at all. Thank goodness I hadn't put the heaters up yet or
> reversed the
> ceiling fans.
> --
> Pam Evans
> Kemp TX
> zone 8A
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Lot 373: A Set of 6 Victorian Carved Oak Single Chairs having barley twist
Antiques & Fine Art Auction
by P F Windibank
October 22, 2016
Dorking, United Kingdom
Past Lot
No Image Available
Looking for the realized and estimated price?
Description: A Set of 6 Victorian Carved Oak Single Chairs having barley twist supports, carved pierced splat backs with grape and vine decoration, rush seats on round turned and twist legs (2 twist backs a/f and 1 slat missing)
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News & Politics, Society & Culture, HistoryFollow
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August 5th, 2014
Episode 722 of 1418 episodes
In Colombia's Marxist guerrilla war, thousands of rebel fighters have been female. When they got pregnant, they were forced to have abortions or give their babies up. Now, many of these rebel mothers have demobilised and are desperate to find their children.
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From Heavy Hammer: ***Now Available for iPad******Google Maps and Directions***USHUD.com is a totally FREE list of foreclosures. We list properties owned by the government and all major banks. We give Realtors & Lenders an exclusive opportunity to help homebuyers purchase homes in their local area.USHUD.com is a private company not affiliated, endorsed, authorized, or licensed by the US Department of Housing and Urban Development.
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Click the button below or email firstname.lastname@example.com to contribute your experiences with this product. Although not necessary, it's interesting to have a first name, city, state and country (no email addresses will be used). Any information submitted becomes the property of Tennis Warehouse.
Please include any relevant information such as skill level and style of play.
Comments: I bought these on cyber Monday and started playing with these shoes on December 2. A mere five
weeks later, and the outside of the sole is already worn down to the plastic support on the widest portion of the toe box. Most of
the outside tread is worn completely smooth. I am 59 years old and play mostly doubles. I will send them back to get another pair.
Also, the tongue is so short I can barley tie the laces without them touching my socks. Glad TW will honor the New Balance
From: Joel, 1/15
Comments: Perhaps the worst NB shoe I have ever tried on and wouldn't think of ever attempting to wear them on
court. Really odd fit compared the old lasts they used to use, cheap materials and didn't look well constructed. Frankly, haven't
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Comments:I have worn nothing but New Balance size 9EE shoes for more than a decade. Either the shoes sent to
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9EE shoes and the difference in width was striking.
From: Rick, 8/14
Comments: I have worn New Balance Tennis Shoes for a long time. The 2E width in this shoe does feel different
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Welcome to Normandy! This small and intimate holiday park is situated only 800 metres from the beach in the little village of Sotteville-sur-Mer.
The resort has been built in the typical architectural style of the region to fit in with Sotteville-sur-Meer's status as a UNESCO World Heritage Site!
The resort is extremely child-friendly. The carparks for guests are all located outside of the park's grounds. There is a luggage cart that you can use to bring your suitcases to your accommodation. Le Pré Marin Holiday Park is also well suited for wheelchair users.
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|"Jukka Zitting (JIRA)" <j...@apache.org>
|[jira] Updated: (JCR-1877) the spi2dav sandbox project should be put into a common release cycle
|Mon, 24 Nov 2008 14:52:46 GMT
[ https://issues.apache.org/jira/browse/JCR-1877?page=com.atlassian.jira.plugin.system.issuetabpanels:all-tabpanel ] Jukka Zitting updated JCR-1877: ------------------------------- Component/s: (was: jackrabbit-spi) sandbox Issue Type: New Feature (was: Bug) Angela, what's the current status in spi2dav? Should we target at releasing it in Jackrabbit 1.6? > the spi2dav sandbox project should be put into a common release cycle > --------------------------------------------------------------------- > > Key: JCR-1877 > URL: https://issues.apache.org/jira/browse/JCR-1877 > Project: Jackrabbit > Issue Type: New Feature > Components: sandbox > Affects Versions: jcr-server 1.4.1 > Reporter: Ken Yue > > Remoting JSR170 calls requires obviously both server and client sides. The server is available for download, as JCR WebDAV Server in both http://www.apache.org/dyn/closer.cgi/jackrabbit/binaries/jackrabbit-jcr-server-1.4.1.jar and http://www.apache.org/dyn/closer.cgi/jackrabbit/binaries/jackrabbit-webapp-1.4.war. However, the client is just casually mentioned as "can be found in the Jackrabbit sandbox." This issue is to request that the SPI2DAV client code (especially a ResponseFactory that returns a JCR2SPI Repository implementation) be available for download as well. -- This message is automatically generated by JIRA. - You can reply to this email to add a comment to the issue online.
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by Elizabeth Schneider
Vegetables from Amaranth to Zucchini: The Essential Reference is at once an encyclopedia, a produce market manual, and a treasure trove of recipes. With produce specialist Elizabeth Schneider as your guide, take a seed-to-table voyage with more than 350 vegetables, both exotic and common. Discover lively newcomers to the North American cornucopia and rediscover classic favorites in surprising new guises.
In this timely reference, Elizabeth Schneider divulges the secrets of the vegetable kingdom, sharing a lifetime of scholarly sleuthing and culinary experience. In her capable hands, unfamiliar vegetables such as amaranth become as familiar as zucchini—while zucchini turns out to be more intriguing than you ever imagined.
Each encyclopedic entry includes a full-color identification photo, common and botanical names, and an engaging vegetable "biography" that distills the knowledge of hundreds of authorities in dozens of fields—scientists, growers, produce distributors, and chefs among them.
Practical sections describe availability, selection, storage, preparation, and basic general use. Finally, the author's fresh contemporary recipes reveal the essence of each vegetable and a culinary sensibility that food magazine and cookbook readers have trusted for thirty years. Each entry concludes with a special "Pros Propose" section—spectacularly innovative recipes suggested by professional chefs.
Vegetables from Amaranth to Zucchini: The Essential Reference is an indispensable resource for home cooks, food professionals, gardeners, information seekers, and anyone who simply enjoys good reading.
Vegetables from Amaranth to Zucchini:
The Essential Reference
by Elizabeth Schneider
Morrow Cookbooks/HarperCollins Publishers
Hardcover, 804 pages
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