text:
Seafood Shells
27 Aug 2009
|White fish
|1 Can (10 oz), drained, flaked
|Medium shrimp
|1 Can (10 oz), drained
|Pearl onions
|1⁄2 Cup (8 tbs) (Cooked)
|Chopped sweet pickles
|1⁄2 Cup (8 tbs)
|Sliced pimiento stuffed olives
|1⁄2 Cup (8 tbs)
|Chili sauce
|1 Cup (16 tbs)
|Prepared horseradish
|2 Tablespoon
|Worcestershire sauce
|1⁄2 Teaspoon
|Tabasco sauce
|To Taste
|2 Quart
|1⁄2 Teaspoon
|Macaroni shells
|12 Large (Uncooked)
|Parsley sprigs
|1 (For Garnish)
|Lettuce leaves
In a large bowl, toss tuna fish, shrimp, onions, pickles and olives.
In a small bowl, mix chili sauce, horseradish, Worcestershire sauce and Tabasco sauce.
Gently fold into seafood mixture.
Cover and refrigerate while preparing shells.
Bring water to a rapid boil in a heavy 4 quart saucepan.
Add salt.
Gradually add shells, being sure water continues to boil.
Cook shells uncovered until tender but quite firm, stirring occasionally.
With a slotted spoon, remove shells from water.
Invert shells on a dry cloth towel; drain 5 minutes.
Fill with chilled seafood mixture.
Serve immediately or cover with plastic wrap and refrigerate several hours before serving.
Garnish each shell with a sprig of parsley.
Serve on lettuce leaves.
Makes 12 shells.
Rate It
Seafood Shells Recipe


text:
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text:
Science, Technology, and National Socialism
Edited by Monika Renneberg Mark Walker
Format: Hardback
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Science, Technology, and National Socialism by Monika Renneberg
Book DescriptionThis 1993 book provides a survey of the development of scientific disciplines and technical projects under National Socialism in Germany. Each contribution addresses a different aspect which is important for judging the interaction between science, technology and National Socialism. In particular, the personal conduct of individual scientists and engineers as well as the functionality of certain theories and projects are examined. All essays share a common theme: continuity and discontinuity. All authors cover a period from the Weimar Republic to the post-war period. This unanimity of approach provides answers to major questions about the nature of Hitler's regime and about possible lines of continuity in science and technology which may transcend political upheaval. The book is also the most comprehensive to date on this subject, and includes essays on engineering, geography, biology, psychology, physics, mathematics, and science policy.
Buy Science, Technology, and National Socialism book by Monika Renneberg from Australia's Online Bookstore, Boomerang Books.
Book DetailsISBN: 9780521403740
(234mm x 158mm x 29mm)
Pages: 444
Imprint: Cambridge University Press
Publisher: Cambridge University Press
Publish Date: 11-Nov-1993
Country of Publication: United Kingdom
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Books By Author Monika Renneberg
Science, Technology, and National Socialism, Paperback (September 2003)» View all books by Monika Renneberg
A comprehensive 1993 survey of the development of scientific disciplines and technical projects under National Socialism.
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text:
This is just a fantastic photo capture of a Mother and Baby Rhinoceros!
Too Cute for Words! Please share our site and posts with your friends!
VN:F [1.9.22_1171]Absolutely adorable Baby Rhino,
More Cute Baby Animals:
Fatal error: Uncaught Exception: 12: REST API is deprecated for versions v2.1 and higher (12) thrown in /home/babyanim/public_html/wp-content/plugins/seo-facebook-comments/facebook/base_facebook.php on line 1273


text:
- Players
- 2
- Genre
- Puzzle
- Release Date
Wii Shop
- 24th Sep 2007, 800 points (USA)
- 27th Jul 2007, 800 points (UK/EU)
- Series
- Kirby
- Controller Support
- Classic, Gamecube
- Also Known As
- Kirby's Ghost Trap
About This Game
The call has gone out throughout Dream Land – the First Annual Ghost Trap Competition has begun!
In this fast-paced puzzler it's up to you to battle Kirby's adversaries by strategically stacking colour-coded Blobs and blending them together to form massive chain reactions.
Each colossal explosion you create will cause a bombardment of Ghosts to rain down upon your opponent. Through 16 levels of furious action you'll face famous enemies from the Kirby universe as you make your way towards a landslide victory!


text:
I'm baaack! Oh sweet mercies the roads I have traveled these last two years. Ups and downs. Joys and disappointments. Life abundantly. I will say this isn't my usual kind of post. At least not of late. Not that I've posted much lately, but you know. I meant to. Being distracted by a side dish of…
Category: Wordless Wednesdays
Wordless Wednesdays: Handel's Messiah
I didn't grow up in the church. I never went to Sunday School, Vacation Bible School, or Egg Hunts. I never even got to be in a Christmas Pageant. Ever. I cannot begin to explain what it felt like as a kid, to see the neighborhood churches bustling with activity and knowing I couldn't…
Wordless Wednesdays: 1989
It was 80's week on Facebook last week and we were asked to post pictures of ourselves from that hilarious decade. Do any of you remember the Aqua-Net fog in the girls locker room? I still have hair big enough to have its own zip code. My scanner isn't working, but I do have a picture of…
Wordless Wednesday: It's That Hot
Sent from a friend. Thought ya'll would enjoy the funny.
Wordless Wednesdays: Botanical Gardens, Chicago
Just some random shots from our visit to the Botanical Gardens in Chicago, IL. It's been almost 22 years since we've been there. I'd forgotten how beautiful it is.


text: OverviewBack to top Green Pharmaceuticals - Snore Stop Allergy Formula - 20 Tablets Item# :62362UPC# :769682267773 Brand:Green Pharmaceuticals Size/Form:20 Tablets Servings:20 Dosage:1 Tablet(s) Snore Stop Allergy Formula Tablets are specially formulated to stop or reduce snoring symptoms associated with allergies and hay fever. Allergy Formula is a special formula designed for those wh9o still need additional support after using the SnorStop Maximum Strength Formula.The Snore Stop Allergy Formula works efficiently on both the nasal and back of the throat areas by shrinking the swollen tissues of the soft palate, tonsils and uvula and by drying nasal secretions obstructing the upper respiratory passages.Snore Stop Allergy Formula contains twice as many natural ingredients. Its deeper and broader mode of action may provide faster and longer relief. You can prolong the benefits of Snore Stop Allergy Formula by changing your position during sleep, dieting, losing weight, exercising more often, avoid or curb smoking, and limit or avoid alcohol and over-the-counter sleeping pills.(Shown to be effective for 80% of those tested. Sleep and Breathing, Volume 3 No. 2, 1999)Promotes all night relief of snoring symptoms.pills.


text:
Media Overload & Zombie Munchies
by, 04-11-2013 at 07:50 AM (588 Views)
Do you watch the News?
I go in spurts, listening in the morning to the same ol' same ol' - North Korea is going to bomb us/ Nuclear war is impending, shootings here, stabbings there, tornadoes widespread here, hurricane there, allergy season WORST in history, Flu outbreak Most severe in Decades, blah blah freaking blah. Every year it's the same thing, over and over. This is the worst, most severe, terrabilist (I know that's NOT a real word) ever . . .
Then it occurs to me that I've become completely desensitized to their daily rhetoric. 9x's out of 10 the media blows things so far out of proportion that I barely believe Anything I see or hear on the news any longer. My kids, unfortunately, have not been completely desensitized to the daily dose of BS that blares on the tv & radio and our youngest is full of questions about Korea & the very real possibility of Nuclear War and Fallout. I find him searching websites, reading articles from various other countries newspapers/websites, etc in order to get a complete picture of what is really happening.
Do you watch the news? How much of it do you "believe" and does it change how you shop, live your daily lives, etc?
I have to admit, I did get extra seeds this year and avoided varieties of hyrbid seeds that may or not create "dead" seeds. I want to be able to harvest the seeds from this years fruits/veggies to plant next season- because quite frankly, I don't want to rely on whether or not I can buy them downtown.
Which brings me to another question... do Hybrid/ genetically engineered foods bother you? I have to say that I don't particularly care for them. For most of the foods that I choose to eat, I do so for a reason. I like fresh corn (& the cornsilk) which is great for a number of conditions, but if it's been genetically modified- how do we know that those beneficial elements are still in it? At what point does their "genetically engineered" stop being Real food and become little more than zombie munchies... after all, do you really know which nutrients they've bred OUT of the food you're eating?! Are you willing to take their word for it??
Do you wish that the Government would Label these so-called Zombie foods and if they did, would it change how & what you purchase?
Food For thought, over and out.


text:
Histone deacetylase inhibition alters dendritic cells to assume a tolerogenic phenotype and ameliorates arthritis in SKG mice
DOI: 10.1186/ar3339
© Misaki et al.; licensee BioMed Central Ltd. 2011
Received: 16 September 2010
Accepted: 18 May 2011
Published: 18 May 2011
The purpose of this study was to elucidate the effects of histone deacetylase inhibition on the phenotype and function of dendritic cells and on arthritis in SKG mice.
Arthritis was induced in SKG mice by zymosan A injection. Trichostatin A, a histone deacetylase inhibitor, was administered and its effects on arthritis were evaluated by joint swelling and histological evaluation. Interleukin-17 production in lymph node cells was determined by an enzyme-linked immunosorbent assay (ELISA). Foxp3 expression in lymph node cells and the phenotypes of splenic dendritic cells were examined by fluorescence-activated cell sorting (FACS). Bone marrow-derived dendritic cells (BM-DC) were generated with granulocyte macrophage colony-stimulating factor. The effects of trichostatin A on cell surface molecules, cytokine production, indoleamine 2,3-dioxygenase (IDO) expression and T cell stimulatory capacity were examined by FACS, ELISA, quantitative real-time polymerase chain reaction and Western blot, and the allo-mixed lymphocyte reaction, respectively.
Trichostatin A, when administered before the onset of arthritis, prevented SKG mice from getting arthritis. Trichostatin A treatment also showed therapeutic effects on arthritis in SKG mice, when it was administered after the onset of arthritis. Trichostatin A treatment reduced Th17 cells and induced regulatory T cells in lymph node, and also decreased co-stimulatory molecule expression on splenic dendritic cells in vivo. In vitro, trichostatin A markedly suppressed zymosan A-induced interleukin-12 and interleukin-6 production by BM-DC and up-regulated IDO expression at mRNA and protein levels. Trichostatin A-treated BM-DC also showed less T cell stimulatory capacity.
Histone deacetylase inhibition changes dendritic cells to a tolerogenic phenotype and ameliorates arthritis in SKG mice.
Rheumatoid arthritis is a chronic inflammatory disorder, characterized by cellular infiltration of and proliferation in the synovium, leading to the progressive destruction of the joints. Dendritic cells, monocytes, T cells, B cells, and neutrophils infiltrate the synovium and interact with each other to induce chronic synovitis [1, 2].
Dendritic cells are efficient antigen-presenting cells, and develop innate and adaptive immune responses through interactions with T cells [3]. Dendritic cells determine the fate of T cell differentiation through the cytokines they produce; IL-12 induces Th1 cells, the combination of IL-6, IL-23, and TGF-β induces Th17 cells, and TGF-β induces regulatory T cells (Treg) [3, 4]. Recently, Th17 cells have been shown to play a major role in both human and mouse arthritis [5–7]. Moreover, CD4+ T cells activated by dendritic cells express RANKL and facilitate osteoclast development, leading to bone erosion in joints with rheumatoid arthritis [8]. It is hypothesized that dendritic cells are activated by unknown stimuli in peripheral tissues, and migrate into the lymph nodes, where they induce T cells to proliferate. Activated T cells, as well as dendritic cells, migrate into the joints and induce inflammatory processes, including the production of cytokines such as TNF-α, IL-1, and IL-6, resulting in the characteristically pathological joint damage [9]. In fact, dendritic cells accumulate in the perivascular area in close association with T and B cells in the synovium of joints with rheumatoid arthritis [10]. Thus, dendritic cells are thought to be involved in both initiating and shaping the immune responses in rheumatoid arthritis pathology.
Dendritic cells have been shown to regulate as well as elicit the immune response; those cells with regulatory properties are called tolerogenic dendritic cells. The tolerogenic dendritic cells regulate the immune responses by inducing T cell anergy, inducing Treg, or activating Th2 cells [11]. The characteristics of tolerogenic dendritic cells are as follows: 1) lower expression of cell surface molecules such as CD80 and CD86, 2) a higher expression of indoleamine 2,3-dioxygenase (IDO), 3) decreased secretion of cytokines related to the innate immune response, and 4) lower T cell stimulation capacity [12, 13]. Various attempts have been made to generate tolerogenic dendritic cells and endogenous- or in vitro-generated tolerogenic dendritic cells have been injected in vivo for treating autoimmune disease, illustrating that dendritic cells are now considered as target cells in inflammatory conditions [14].
Histone deacetylase inhibitors (HDAi), such as trichostatin A (TSA) and suberoylanilide hydroximic acid, are small molecule compounds that exert anti-proliferative effects on various tumor cells and are currently used as anti-cancer drug [15]. Histone deacetylase inhibitors are also potential therapeutic agents for rheumatoid arthritis because HDAi suppress joint swelling, synovial inflammation, and subsequent bone and cartilage destruction in animal models of rheumatoid arthritis [16–18]. The mechanism of anti-rheumatic activity by HDAi has been ascribed to the suppression of proliferation and function of synovial fibroblasts. In fact, we have shown the growth-inhibitory effects of HDAi on rheumatoid arthritis-synovial fibroblasts in vitro [19]. Recently, however, HDAi have been reported to have immunoregulatory effects along with anti-tumor effects. We and others have shown that HDAi alter the phenotype and cytokine production of dendritic cells, as well as differentiation of monocytes into dendritic cells [20, 21].
To clarify the immunoregulatory role of HDAi in a mouse arthritis model, we examined the effect of an HDAi (TSA) on SKG mice, a T cell-mediated model of chronic arthritis. We also examined the effects of TSA on the phenotypes and functions of mouse bone marrow-derived dendritic cells (BM-DC). Here, we show the regulatory effects of TSA on dendritic cells in vitro, as well as the preventive and therapeutic effects on arthritis in vivo.
Materials and methods
Female SKG mice and female C57BL/6 mice were obtained from CLEA Japan, Inc. (Osaka, Japan). Both the SKG and C57BL/6 mice were housed in the Kobe University animal facility at a constant temperature and were provided laboratory chow and water ad libitum. All procedures were carried out in accordance with the recommendations of the Institutional Animal Care Committee of Kobe University.
Reagents and antibodies
Zymosan A (ZyA), dimethyl sulfoxide (DMSO), phorbol myristate acetate (PMA), suberoylanilide hydroximic acid, ionomycin, bovine serum albumin, 2-mercapto-ethanol (2-ME), and saponin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was from Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan). Trichostatin A (TSA), 4% paraformaldehyde phosphate buffer solution, hematoxylin and eosin, RPMI-1640 with L-glutamine, phenol red, and HEPES were from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). EDTA (Dojindo Laboratories, Kumamoto, Japan), fetal bovine serum (MP Biomedicals, Inc., Illkirch, France), 1% penicillin-streptomycin (Lonza Walkersville, Inc., Walkersville, MD, USA), recombinant murine granulocyte macrophage colony-stimulating factor (Peprotech, Rocky Hill, NJ, USA) were also purchased. The PE-anti-mouse FOXP3 Flow kit and allophycocyanin (APC)/Cy7-anti-mouse CD8a were purchased from BioLegend, San Diego, CA, USA. Phycoerythrin (PE)-anti-mouse CD80, PE-anti-mouse CD86, PE-anti-mouse CD40, PE- anti-mouse MHC class II (I-E(k)), fluorescein isothiocyanate (FITC)-anti-mouse B220, FITC-anti-mouse CD25, FITC-anti-mouse CD80, FITC-anti-mouse CD86, FITC-anti-mouse CD40, FITC-anti-mouse MHC class II (I-A/I-E), FITC-anti-mouse CD54, allophycocyanin (APC)-anti-mouse CD11c, and APC-anti-mouse CD4 were purchased from eBioscience (San Diego, CA, USA).
Induction of arthritis
SKG mice that were seven or eight weeks old were intraperitoneally injected with 2 mg/mouse ZyA, as previously described [22]. Briefly, ZyA suspended in saline was kept in boiling water for 10 minutes and the ZyA solution (0.5 ml/mouse) was intraperitoneally injected into SKG mice. Arthritis developed between 14 and 21 days after injection.
Treatment of SKG mice with trichostatin A
Trichostatin A (8 mg/kg) was dissolved in DMSO and subcutaneously administered to SKG mice from Day 14 (before the onset of arthritis) to Day 22 (after the onset of arthritis). DMSO was used as a control.
Evaluation of arthritis
Arthritis severity was evaluated according to the clinical arthritis scores as follows: 0, no joint swelling; 0.1, swelling of one finger joint; 0.5, mild swelling of wrist or ankle; 1.0, severe swelling of wrist or ankle, as previously reported [23]. Arthritis scores for all the digits of the forepaws and hind paws, as well as for the wrists and ankles, were totaled for each mouse. The maximum possible clinical arthritis score is 5.8.
Mice were killed on Day 35 after the administration of ZyA. Control mice injected with DMSO were killed at the same time. After the groups of mice were killed, their hind paws were removed, fixed in 4% paraformaldehyde in phosphate-buffered saline, decalcified in EDTA, embedded in paraffin, and sectioned. The samples were then stained with hematoxylin and eosin. Histologic evaluation was performed by the scoring system described previously, in which 0 = no inflammation, 1 = slight thickening of the synovial cell layer and/or some inflammatory cells in the sublining, 2 = thickening of the synovial lining, infiltration of the sublining, and localized cartilage erosions, and 3 = infiltration in the synovial space, pannus formation, cartilage destruction, and bone erosion [24].
IL-17 production and Foxp3 expression in SKG mice
Inguinal lymph node cells (1.0 × 106 cells/ml) were collected and stimulated with PMA + ionomycin and the IL-17A levels in the supernatants were determined by an enzyme-linked immunosorbent assay (ELISA) (SABiosciences, Frederick, MD, USA), following the manufacturer's instructions. Inguinal lymph node cells from both the DMSO-treated and TSA-treated SKG mice were collected on Day 35 and then were ground using the inner cylinder of a syringe on the cell strainer (BD Biosciences Pharmingen, San Jose, CA, USA) in a 3.5-cm Petri dish. The cells were stained with APC-anti-CD4, FITC-anti-CD25, and PE-intracellular Foxp3 monoclonal antibodies according to the manufacturer's protocol. Foxp3 expression on gated CD4+CD25+T cells was determined by flow cytometry.
Analysis of conventional splenic dendritic cells in SKG mice with fluorescence-activated cell sorting
Splenic cells from both the DMSO-treated and TSA-treated SKG mice were collected on Day 35 and treated with ACK lysing buffer (Lonza Walkersville, Inc.) to lyse red blood cells at 4°C for five minutes, followed by washing twice with 0.5% bovine serum albumin in phosphate-bufferd saline. The cells were incubated with the indicated monoclonal antibodies (FITC-anti-B220, APC-anti-CD11c, APC/Cy7-anti-mouse CD8a and PE- anti-CD80 or PE-anti-CD86 or PE-anti-CD40 or PE-anti-MHC class II) for 30 minutes at 4°C. Isotype-matched antibodies were used as controls, and Fc block (BD Biosciences Pharmingen) was used to block non-specific binding to Fc receptors. After extensive washing, the cells were stained with 7AAD (BD Biosciences Pharmingen). The cells were analyzed on a FACSCalibur or a FACS Canto II flow cytometer (Becton Dickinson, San Jose, CA, USA) at the CD11c high-B220 negative gate to define conventional dendritic cells. Data were expressed as the mean fluorescence intensity and/or as the percentage (%) of positive cells after subtraction of background isotype-matched values.
Generation of bone marrow-derived dendritic cells
Bone marrow-derived dendritic cells (BM-DC) were generated from SKG mice. Briefly, bone marrow cells were collected from the SKG mouse femur, and 1.0 × 106 bone marrow cells were cultured in a 24-well plate with RPMI-1640 supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 100 μM 2-ME, and 50 ng/ml recombinant murine granulocyte macrophage colony-stimulating factor. The medium were changed every two days. On Day 8, weakly adherent cells were harvested using 4°C PBS as BM-DC [25].
Cell surface molecules of bone marrow-derived dendritic cells
Bone marrow-derived dendritic cells were generated as mentioned above and stimulated with ZyA (5 μg/ml), TSA (20 nM), or ZyA+TSA for the last 48 h. Cells were harvested and incubated with the indicated monoclonal antibodies (APC-anti-CD11c and FITC-anti-MHC class II, FITC-anti-CD54, FITC- anti-CD80, FITC-anti-CD86, or FITC-anti-CD40) for 30 minutes at 4°C. Cells were stained and analyzed at the CD11c high gate using the FACSCalibur as previously described.
Enzyme-linked immunosorbent assay
Bone marrow-derived dendritic cells (1.0 × 106 cells/ml) were stimulated with ZyA (5 μg/ml), TSA (20 nM), or ZyA + TSA for 18 h and the IL-12p70, IL-12p40, and IL-6 levels in the culture supernatant were measured with commercial ELISA kits (BD Biosciences, San Diego, CA, USA) following the manufacturer's instructions.
Quantitative real-time polymerase chain reaction
Levels of IDO1 and IDO2 mRNA expression were determined by quantitative real-time polymerase chain reaction. Total RNAs were isolated using an RNeasy Mini kit (Qiagen, Tokyo, Japan) and cDNA synthesis was performed using Super Script III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA). Amplification was run in triplicate using an SYBR Green Gene Expression Assay (Qiagen) according to the manufacturer's protocol. The primer pairs used in the reactions were purchased from Qiagen (QT00103936 for IDO1 and QT01066345 for IDO2). The amplification reactions, data acquisition, and analyses were performed with the ABI Prism 7900 HT instrument (Applied Biosystems, Foster city, CA, USA). Glyceraldahyde-3-phosphate dehydrogenase (GAPDH, Qiagen QT01658692) was used as the housekeeping gene against which all of the samples were normalized for differences in the amount of total RNA added to each cDNA reaction and for the variation in the reverse transcriptase efficiency among the different cDNA reactions.
Western blot analysis
Bone marrow-derived dendritic cells were harvested after stimulation with ZyA (5 μg/ml), TSA (20 nM), or ZyA + TSA for 48 h and lysed with RIPA buffer (Thermo Scientific, Rockford, IL, USA) containing protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) at 4°C for 30 minutes. After centrifugation at 12,000 × g for 15 minutes, the supernatants were removed and the protein concentrations were determined using the BCA Protein Assay Reagent (Pierce Chemical Company, Rockford, IL, USA). Samples containing 10 to 30 μg of proteins were boiled for five minutes in sodium dodecyl sulfate sample buffer (Wako Pure Chemical Industries, Ltd.). The expression of IDO was determined by immunoblot analysis using purified anti-mouse IDO antibody (BioLegend).
Allo-mixed lymphocyte reaction
Naïve CD4+T cells from C57BL/6 mice were purified by positive selection using anti-CD4+CD62L magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany).
Bone marrow-derived dendritic cells from SKG mice were harvested and purified by positive selection using anti-CD11c+ magnetic beads (Miltenyi Biotec,).
Naïve CD4+T cells (1.0 × 105/200 μl) were co-cultured with 2.0 × 104 control dendritic cells, ZyA (5 μg/ml) dendritic cells, TSA (20 nM) dendritic cells, or ZyA + TSA dendritic cells derived from SKG mouse bone marrow. On Day 5, cell proliferation was determined by a cell proliferation ELISA kit (Roche, Penzberg, Germany), using BrdU and anti-BrdU antibodies.
Statistical analysis
Results are expressed as the mean ± standard error of the mean (SE). Statistical comparisons were performed by Student's t-test. Differences were considered significant when P < 0.05.
Preventive effects of trichostatin A on SKG mice
The effects of trichostatin A on IL-17 production and Foxp3 expression by inguinal lymph node cells
We also examined whether TSA affected the Treg population in SKG mice. Foxp3 expression in inguinal lymph node cells on Day 35 from the control- and TSA-treated SKG mice were determined by fluorescence activated cell sorting (FACS). We found significant increase in the ratio of CD4 + CD25 + Foxp3+ cells among CD4+ cells in TSA-treated group compared to control group, suggesting that Treg are involved in the prevention of arthritis in SKG mice with TSA (Figure 3B).
The effects of trichostatin A on the phenotype of splenic dendritic cells
The effects of trichostatin A on cytokine production of zymosan A-treated dendritic cells in vitro
The effects of trichostatin A on IDO1 and IDO2 expression in bone marrow-derived dendritic cells
IDO1 and IDO2 expression in BM-DC were determined using real-time polymerase chain reaction. Both IDO1 and IDO2 are rate-controlling enzymes related to tryptophan metabolism and tryptophan depletion in the microenvironment has been reported to suppress cell proliferation [28, 29]. Thus, IDO1 and IDO2 expressions in dendritic cells suppress the T cell reaction through tryptophan depletion. We examined the mRNA expression of IDO1 and IDO2 in BM-DC and found that TSA or ZyA alone induced IDO marginally, but the combination of TSA and ZyA markedly induced mRNA expression of IDO1 and IDO2 (Figure 5B). Western blot analysis confirmed the induction of IDO expression by BM-DC with the combination of ZyA and TSA at the protein level (Figure 5C). Protein expression levels of IDO were similar to the mRNA levels of IDO2.
The effects of trichostatin A on cell surface molecules of bone marrow-derived dendritic cells
The effects of trichostatin A on dendritic cell-induced T cell proliferation
Therapeutic effects of trichostatin A on arthritis in SKG mice
Our results have clearly shown that TSA ameliorates arthritis in SKG mice. The effects were characterized by a down-regulation of Th17 cells as well as up-regulation of Treg. We assumed that dendritic cells play a critical role in this model because it is well known that ZyA activates dendritic cells via the Dectin-1 and Toll-like receptor (TLR)-2 pathway [30, 31]. Considering the significance of dendritic cells in determining Th cell differentiation, we examined the effects of TSA on dendritic cells in vivo and in vitro and concluded that TSA ameliorated arthritis, at least in part, by inhibiting dendritic cell activation.
Some reports have shown a therapeutic effect of HDAi on arthritis in mice; antibody-induced arthritis, collagen-induced arthritis, and adjuvant-induced arthritis have all been successfully treated with various HDAi [16–18]. Previous reports have shown that HDAi induce p21 in synovial fibroblasts, protect against cartilage apoptosis, inhibit matrix metalloproteinase production, and up-regulate Treg in vivo [17, 32, 33]. However, this is the first report to demonstrate that HDAi can ameliorate arthritis in a mouse model through regulating dendritic cells.
Our in vitro experiments indicated that HDAi skewed dendritic cell function to a tolerogenic-like phenotype. Zymosan A induced maturation of BM-DC, up-regulating expression of cell surface molecules, cytokine production, and T cell stimulation. When dendritic cells were stimulated with ZyA in the presence of TSA, a significant decrease was observed in the cytokine production, expressions of co-stimulatory molecules, and T cell stimulatory capacity, and a significant up-regulation of IDO gene and protein expression was also observed. Tolerogenic dendritic cells present antigens to antigen-specific T cells, but fail to deliver adequate co-stimulatory signals for effector T cell activation and proliferation[11]. Trichostatin A-treated dendritic cells in vitro are similar to tolerogenic dendritic cells in that they produce low levels of cytokines and high levels of IDO, but are different in that the expression of co-stimulatory molecules (CD80) is not markedly reduced. Thus, we consider that HDAi alter dendritic cells to a tolerogenic-like phenotype. Some previous reports have reported that histone deacetylase (HDAC) inhibition alters dendritic cell function when lipopolysaccharide was used to stimulate and differentiate dendritic cells [34]. We have found similar results using ZyA, which signals through Dectin-1 and TLR-2, instead of lipopolysaccharide, which utilizes TLR-4, illustrating that HDAC inhibition alters dendritic cell function regardless of the stimulation. Interestingly, activation of the Dectin-1 pathway has been shown to lead to the generation of Th17 cells, rather than Treg, through the syk-CARD9 pathway [35, 36]. It is possible that TSA suppresses the Dectin-1 pathway in dendritic cells, resulting in decreased Th17 cell generation.
Dendritic cells regulate CD4+T cell differentiation and the immune response. Interleukin-12 is a key cytokine in Th1 cell differentiation and IL-6 is key in Th17 cell differentiation [37]. Tumor growth factor-β and retinoic acid induce Treg [38]. Our in vivo results demonstrated that TSA treatment markedly reduced Th17 cell population and slightly up-regulated Treg. Considering a larger effect of TSA on IL-17 production, TSA appears to have ameliorated arthritis in mice primarily by inhibiting the dendritic cell activation by ZyA because it has been shown that TSA and suberoylanilide hydroximic acid suppress Th17 cell differentiation by altering dendritic cell function [27]. Consistent with our results, some reports have shown the induction of Treg by HDAi treatment in vivo [33, 39]. It is difficult to explain how TSA induces Treg in vivo. First, it is difficult to determine which subset of Treg, natural Treg or induced Treg, was derived by TSA in SKG mice [40–43]. Moreover, TSA might directly induce Treg through acetylation of Foxp3 or TSA might modulate dendritic cell function to indirectly induce Treg [44]. We have failed to determine the direct effects of TSA on Th cell differentiation in vitro because TSA suppressed naïve CD4+ T cell proliferation so strongly as to prevent examination of the functional differentiation.
In mice, conventional dendritic cells that reside in lymphoid tissue can be separated into CD8α+ and CD8α- conventional dendritic cells [45, 46]. We observed that TSA treatment in vivo significantly down-regulated co-stimulatory molecules on the CD8α+ conventional dendritic cell subset, but not on the CD8α- conventional dendritic cell subset. Furthermore, TSA tended to decrease the CD8α+ conventional dendritic cell population compared to DMSO treatment, although the difference was not statistically significant (data were not shown). These results suggested that TSA mainly affected the CD8α+ conventional dendritic cell population in vivo. CD8α+conventional dendritic cells are considered a more developed or activated form of CD8α- conventional dendritic cells, because CD8α+ conventional dendritic cells have been shown to more potently induce CD4+T cell proliferation and interferon-γ production compared with similarly activated CD8α- conventional dendritic cells [47–49]. Recently, CD8α+ conventional dendritic cells have been shown to produce IL-12p70 and induce antigen-specific Th17 and Th1 cells, resulting in the acceleration of collagen-induced arthritis [50]. Our results indicated that TSA treatment altered the CD8α+ conventional dendritic cell phenotype to that of the tolerogenic CD8α+ conventional dendritic cells and inhibited Th17 cell differentiation, leading to the suppression of arthritis in SKG mice, in which Th17 cells are critically involved [26]. Thus, we speculate that CD8α+ conventional dendritic cells are one of the targets of the immunoregulatory effects of TSA.
It has been reported that vasointestinal peptide, IL-10, TGF-β, and vitamin D can induce tolerogenic dendritic cells. Histone deacetylase inhibitors are also useful for inducing tolerogenic dendritic cells in the treatment of rheumatoid arthritis, as we have shown in this report. SKG mice do not develop any arthritis in a specific pathogen-free environment, but develop severe arthritis after a single administration of ZyA, indicating that environmental factors contribute to the onset of arthritis [22]. Because microorganisms activate dendritic cells, targeting dendritic cell function is a rational way of regulating the autoimmune response triggered by microorganisms. It is noteworthy that HDAi-treated dendritic cells have been reported to be useful in the treatment of graft-versus-host disease in mice [51]. Thus, the prospects appear promising for dendritic cell-based cell therapy for rheumatoid arthritis using appropriate HDAi.
In conclusion, HDAC inhibition ameliorates arthritis in SKG mice, at least in part, by altering dendritic cell function into the tolerogenic phenotype. The HDAC protein family consists of at least 18 HDACs, including the sirtuin family of HDACs. Recently, HDAC9 was shown to be involved in Treg regulation [39], and HDAC11 was shown to be involved in immune tolerance by its effect on macrophage function [52]. Further understanding of HDAC functions in dendritic cells and the development of selective HDAi are expected to lead to novel therapies that target dendritic cells.
Histone deacetylase inhibition changes dendritic cells to a tolerogenic phenotype and ameliorates arthritis in SKG mice.
bone marrow-derived dendritic cells
dimethyl sulfoxide
enzyme-linked immunosorbent assay
fluorescence activated cell sorting
fluorescein isothiocyanate
histone deacetylase
- HDAi:
histone deacetylase inhibitors
indoleamine 2,3-dioxygenase
phorbol myristate acetate
Toll-like receptor
- Treg:
regulatory T cells
trichostatin A
- ZyA:
zymosan A.
This study is supported in part by a Grant-in-Aid for Scientific Research (No. 21591265) from the Japan Society for Promotion of Science and a grant from the Japan Rheumatism Foundation.
Authors' Affiliations
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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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Comment – April 16, 2015
I had the above schedule two times a week on Monday and Friday with one prep.
Wednesday I had one prep as well.
On Tuesday and Thursday I had the same schedule as above except I had two preps and four classes to teach. But these preps were taking away sometimes for meetings or to cover a class when people quit, went to a training, or they were absent. Mind you before Christmas we lost 8 staff members, then after we lost 7 staff members.
This is the norm here because the first year in this school building we lost 13 staff members before Christmas, and the second year in this building we lost 12 staff members before Christmas.


text:
Clamp Down on Clumping
First understand whats really causing the problem.
Carr [2-4] developed several tests in an attempt to determine the flowability and compressibility of bulk solids. His methods give indices that have had mixed reviews over the last 40 years. Many companies have devised their own internal methods and have published these for others to use — but they're often subjective and depend on the operator's scrutiny. Examples are observation of flow though different orifice sizes, lumping and compression as well as frangibility and friability tests.
Here's a highspotting of the pros and cons of the most common qualitative tests for evaluating clumping potential:
• Carr Flowability Index (ASTM-D6393) [5] — is quick and easy to do (simple equipment) and used extensively, gives a relative indication of how much a powder will compact and the strength of cohesive material, but can't provide quantitative results and isn't useful for time and temperature effects on solids;
• BASF lumping and compression tests (BASF Bulletin TPU 0402) [6] — uses small amount of material (10–15 g) to get qualitative results, but provides limited ability to study the effect of storage time and temperature on flow;
• Flow though an orifice or down a surface — offers qualitative results on how big an orifice needs to be for 50% of solids to flow out of a container (commercial instruments are available (Figure 1); however, many companies have constructed their own devices) or the necessary angle of slide (specific to wall material), but can't give the effect of storage time on flow; and
• Frangibility (sometimes called friability) tests — indicate relative strength of agglomerates, can be used to estimate crushing strength or how easily a clump can be broken, which may eliminate the need to solve a clumping problem, but use non-standard equipment (large balls are placed on the upper screen of a sieve stack and vibrated; amount of solids that pass to the pan is compared to the amount that goes to the pan without the balls).
In general, these tests will give an indication of the potential for clumping to occur upon storage when starting particulate solids are cohesive or flow poorly, i.e., Carr flowability index of less than 50 (see Table 1). However, initial good flowability doesn't predict lack of clumping upon storage. So, when facing a problem, it's important to look at the 10 most common sources of agglomeration to ascertain the likely culprit(s). In that respect the following techniques are useful in determining potential for phase change and can identify chemical components that are the cause of the problem:
• Raman spectroscopy — can determine polymorphs;
• Differential Scanning Calorimetry (DSC) — can identify changes in structure and presence of unstable chemical forms or polymorphs;
• Scanning Electron Microscopy — can see formation of bridges (this can be combined with a probe to look at specific elements);
• Transmission electron microscopy — can detect bridge composition and impurities (but has limited application unless sample is very strong because it must be thin); and
• Atomic force microscopy— can observe real-time agglomeration (this technique is evolving rapidly and eventually may be able to see atomic-level changes).
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"It's a parable, it's a fable. It's almost like an intellectual Twilight Zone , ... In a way it's like a Hitchcock picture and Tim Roth is the Jimmy Stewart, the guy who gets caught up in something fascinating and big."
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By Mechwing 1 Comments
This is a Test. and just a Test.
This is a Test. and just a Test.
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OpenCart theme design
This project received 29 bids from talented freelancers with an average bid price of $265 USD.Get free quotes for a project like this
Project Budget$30 - $250 USD
Total Bids29
Project Description
We have an online e-commerce store hosted on OpenCart with a ready made theme.
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The site will have almost the same functionality. It will be t-shirt selling e-commerce site only.
There will be a section for designers to submit their designs and other can vote for the designs.
Payment gateway etc will be taken care by us. You just need to develop the design.
An appropriate bid will be highly appreciated.
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text:
Quilt Show entries sought
The second annual Quilt Show hosted by the Sew Happy Quilters Guild and Rumpled Quilts Guild will be held Aug.3-20 at the AGU Everett Street Gallery in Morgan City.
A reception will be held on Aug. 4 from 6 p.m. to 8 p.m. Snacks will be provided by Mada and Don Tellman. The free event is open to the public.
The Everett Street Gallery is located at 201 Everett St.
Gallery hours are Wednesdays through Fridays 1 p.m. to 4 p.m. and Saturdays 10 a.m. to 1 p.m.
Anyone with a quilt they would like to enter into the show should contact the Quilt Cupboard at 354-0030 before the deadline of noon on Friday.
- Log in to post comments


text:
I posted another thread, sorry about that. Hopefully the pats* fall into obscurity. Would be funny if the favre lead jets get the division.
I posted another thread, sorry about that. Hopefully the pats* fall into obscurity. Would be funny if the favre lead jets get the division.
No way dude!!!Originally Posted by frunko1
Christmas is coming...and so are the Bills...
Not a real big fan, but if anyone must win the AFC East, I usually pull for the Bills. Got some good friends there so I keep an eye on them for my peeps there.
Steelers...On The STAIRWAY TO SEVEN!!!
ASIAN... thanks, brotha!
I've been telling a co-worker (Pats* fan) all offseason that he could expect Brady to miss games this year. That the NFL would handle the penalty for Spygate in-house and that the first injury he got would be "out for the season".
It'll make for an interesting conversation tomorrow at work.
2013 MNF Executive Champion!
2017 Mock (Revised)
1. Takkarist McKinley, OLB, UCLA
2. Joe Mixon, RB, Oklahoma
3. Jake Butt, TE Michigan
I hate being this voice because honestly I loathe the Pats...but I do believe what goes around comes around. Especially when celebrating the injury of another teams QB. All I'm saying here...is be careful.
1 Takkarist McKinley-OLB UCLA
2 Budda Baker-FS Washington
3 Noah Brown-WR tOSU
4 Joe Mixon-RB Oklahoma
5 Ahkello Witherspoon-CB Colorado
6 Riley Bullough-ILB Michigan State
7 QB-Seth Russell-Baylor
Man guys don't upset the football Gods by celbrating the injury to Tom Brady. The Steelers played a great game today and beat the snot out of the Texans; what happens anywhere else is irrelevant, it sounds like you don't believe the Steelers are good enough to beat the Pats at full strength.
Chill on the happiness seeing Brady hurt and celebrate the Steeler domination of the Texans. The Steelers need to take care of their own business and the rest will fall into place regardless of who is playing and who isn't.
1.30) Taco Charlton, DE, Michigan (McKinley or Williams)
2.62) Quincy Wilson, CB, Florida (Obi Melifonwu, S)
3.94) Noah Brown, WR, OSU
The referee said that you hit Brian Sipe too hard. Did you hit him too hard?
I hit him as hard as I could - Jack Lambert
Originally Posted by ShawnMedGuy
I will knock on wood for my thoughts about this Brady situation.


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My hostas are in pots too. Temp outside in winter can be between
10 and 60 F in The Netherlands, but usually it is at 25-50 F. There
are no problems with breaking dormancy too early.
Ben J.M.Zonneveld
Clusius lab pobox 9505
2300 RA Leiden
The Netherlands
To sign-off this list, send email to email@example.com with the
message text UNSUBSCRIBE HOSTA-OPEN


text:
|Property type
|Minimum Stay
|3 - 6 nights
Sol Seeker is a charming beach home located in the private resort community of Sunset Beach. Sunset Beach is situated on the gulf side of 30a - directly between the beach communities of Alys and Rosemary Beach. Immediately upon entering the private and gated courtyard, you will begin to notice all of the special touches which will make you feel welcomed.
A Map of the neighborhood with the location of the home and the proximity to the beach can be seen by clicking on the Location Tab - you are merely steps to the beach and pool!
The courtyard has been nicely designed to provide you with an oasis offering a pleasant respite for morning coffee or a romantic dinner.
The main floor features an open floor plan with travertine floors, granite counter-tops, tumbled marble back splashes, stainless appliances and a beautiful stained glass window.
One of the greatest amenities of this home are the built in screens for all four sets of french doors which will allow you to open the doors and enjoy the ocean breeze!
The living/dining area has comfortable seating and a flat-screen HDTV over the gas fireplace! A Bose stereo is provided as well. There is a full sized bathroom downstairs with walk-in shower. There is a queen sized sofa sleeper which is very comfortable - not your typical sofa sleeper!
The upstairs has beautiful hardwood floors and eye-catching local art work. There you will find the two bedrooms, both with comfortable, queen-sized beds and french doors with private access to the balcony. There are two, beautiful bathrooms upstairs with travertine showers.
The balcony is fabulous with glimpses of the ocean! There are screens available which can be lowered, offering you shade from the sun to elongate your time spent on the balcony or to provide privacy.
This owner has thought of everything you will need for your time at the beach! You will not want to leave!
- High Speed Internet (WiFi)
- Secluded courtyard home with parking for two vehicles
- Courtyard Dining Table with Umbrella
- Two Queen Bedrooms (one with Adjoining Bathroom)
- Balcony with glimpses of the Gulf
- Bar seating for three with dining table for four
- Small Grill that uses Coleman Propane Pony "Camping" Tanks (not provided)
- Outdoor Shower
- Stackable Compact Washer/Dryer
- Full Bathroom on first floor
- Queen Sofa Bed
- Private Community Pool (Heated seasonally during Cold Months)
- Gated, Security Manned Neighborhood
- This home is Not Pet Friendly (No Exceptions)
• A 50% Advance Deposit is due at the time of booking.
• Balance is due 30 days prior to arrival date.
• With the exception of a cancellation fee, deposits are fully refundable if cancellations are given 90 days prior to arrival date.
• We accept e-checks or credit cards (Additional fee applies for all credit card transactions).
• Check-In begins at 4:00PM CST. If available, an early check-in may be requested for an additional fee.
• Check-Out is by 10:00AM CST. If available, a late check-out may be requested for an additional fee.
• Must Be 25 Or Older To Rent. We have a strict family only policy. No exceptions.
Call, text or email for more information or to book this beautiful property.
I love this place - What a joyous little beach house - Just a wonderful place to be!
Sol Seeker provided a perfect balance of fun and relaxation for my family of 5 (husband, myself, and 3 babies under 3 years old). The beach and pool are close by, making our numerous trips back and forth more than manageable. The fenced in courtyard allowed the kids to explore safely while we sat at the patio table enjoying the outdoors. Renting bikes allowed us to explore the town and dine out without the headache of getting everyone in and out of the car. We have not one complaint, our stay was wonderful. The kids are already asking when we are going back to the "beach house"!
The home was clean and well stocked. It was nice and roomy and very close to the beach. The only improvements that were that the mattress in the room with the bedroom with the bathroom was very uncomfortable and the floors were filthy We couldn't walk barefoot in the home because it would blacken your feet. This is not ideal at the beach.
Beautiful Villa, perfectly located. Incredibly comfortable queen beds.
We had a great stay! We enjoyed being so close to all the Saecrest and Rosemary Beach activities. We will definitely be back next year😊
We thoroughly enjoyed our stay at sol seeker. It was a perfect house and location for our little family. It has a full bath and walk-in laundry room/pantry downstairs. Two bedrooms upstairs, one with its own full bath and one full bath (with tub for those people with children) in the hall. There is a whole bin with beach chairs and beach toys for kids out on the patio too. The complimentary beach chair setup was what really set this rental apart from the others. We were very close to the beach and pool is also right beside the beach. We loved being able to walk out of the neighborhood to Rosemary and Alys Beach. It was a great vacation!
I almost hate to leave a review, because I want to keep this wonderful place to myself! This was our third year staying in Sunset Beach and Sol Seeker was our favorite unit yet! Thoughtful touches like comfortable beds (I hate rentals with cheap, too-firm mattresses... NOT restful on vacation!) blackout curtains (essential for that early sunrise!) and the always-appreciated included chair/umbrella set up sets this place above the rest! I also loved the box of beach goodies on the porch - extra chairs, toys and boogie boards are a nice, personal touch.
We felt like the owners actually use this home and are thoughtful to what goes into it... it's feels like more than just another generic, bare bones rental unit. Personal touches like original artwork make Sol Seeker feel homey and loved. My only wish (and this is a VERY minor one) is that there was more comfortable patio furniture upstairs... I would have liked to spend more time on the upstairs balcony to read and enjoy the partial water views. Some comfy loungers or cushioned seating would have been more than perfect.
We love how convenient you are to the beach and pool (no crossing a street or schlepping on the beach tram) ... so easy to run home for an extra cold drink or snack! And it's such an easy walk to both Rosemary and Alys Beach for dining options. We love Seacrest Sundries (the BEST sandwiches for takeout - pick it up and enjoy lunch by the pool!), Orange Leaf yogurt, La Cocina, Charlie's Donut Truck (daily guilty pleasure), Fonville Press (awesome playground), Wild Olives, Cowgirl Kitchen, Shades, Crabby Steve's and more. We rented bikes at Peddlers 30A one day and had a great time using them but you almost don't even need a bike because it's such an easy walk everywhere, even with a toddler. We are already planning our return trip!
*Plus compulsory or optional charges based on actual occupancy or consumption. The payment has to be made in the currency offered by the property manager, before use of conversion tool. Payments in other currencies have to be negotiated beforehand with the property manager. The currency conversion tool is provided for your convenience only and is non-binding.


text:
Another parade of previous-winner clips. Forest Whitaker presents.
Marion Cotillard wins. Ick. Yuck. As I have said before, I would give this to anyone else in the category, including Laura Linney, and I haven't even seen her performance. Cotillard is way excited. "Thank you, life, thank you, love. It is true there are angels in this city."


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Jason G. Duesing
Provost, Associate Professor of Historical Theology
Jason Duesing serves as the academic Provost and Associate Professor of Historical Theology at Midwestern Baptist Theological Seminary. He came to MBTS after serving for more than a decade on the administrative leadership team and faculty at Southwestern Baptist Theological Seminary in Fort Worth, Texas. His various roles at SWBTS included assistant to the president, chief of staff in the office of the president, and most recently, vice president for strategic initiatives. Duesing also served as Southwestern Seminary's acting provost during the 2011-12 academic year. In the classroom, he was assistant professor of Historical Theology. Duesing earned his Ph.D. in Historical Theology and Baptist Studies from Southwestern Seminary in 2008. He also holds a M.Div. from Southeastern Seminary and a B.A. in Speech Communications from Texas A&M University in College Station.
Duesing serves as a research fellow for the Ethics and Religious Liberty Commission of the Southern Baptist Convention, on the board of directors for the Council on Biblical Manhood and Womanhood, editor of the Journal for Biblical Manhood and Womanhood, academic editor of the Midwestern Journal of Theology, and general editor for For the Church resources. He is also a member of the Evangelical Theological Society and regular contributor to Baptist Press. Duesing is married to Kalee, and together they have four children: Gracyn, Ford, Lindsey, and George.
Local Church:
Antioch Bible Baptist Church, Gladstone, MO
Seven Summits in Church HistoryRainer Publishing, forthcoming
"An Humble Attempt"A Jonathan Edwards Encyclopedia (Eerdmans, forthcoming)
"A Biblical View of the Nations"The Worldview Study Bible (B&H Academic, forthcoming)
"A Puritan's Plea for the Simplicity of Preaching,"But We Preach Christ Crucified: Preachers on Preaching (For the Church, 2015)
"A Wrinkle on Catholicism: The Anglican Understanding of Church Government"Shepherding God's Flock: Church Leadership in the New Testament and Beyond (Kregel, 2014)Learn More
"Standing Like a Steersman in a Storm: Courage to Act Like Men in a Culture that Says Otherwise"Nelson's Annual Preacher's Sourcebook, Vol. 4 (Thomas Nelson, 2014)Learn More
"A Plea for Ambitious Evangelicals"Union University Pulpit, (2013): 43-51.
Adoniram Judson: A Bicentennial Appreciation of the Pioneer American MissionaryB&H Academic, 2012Learn More
Upon this Rock: The Baptist Understanding of the ChurchB&H Academic, 2010Learn More
"The Reclamation of Theological Integrity"L. Russ Bush III and Southeastern Baptist Theological Seminary, 1989-1992 (Christian Higher Education, 9:3 (July 2010): 185-206)
Restoring Integrity in Baptist Churches(Kregel, 2008)Learn More
First Freedom: The Baptist Perspective on Religious Liberty(B&H Academic, 2007)Learn More


text:
Smith & RC Accessories
Showing 1 - 4 items
A scaled down version of Smith's successful Serpico comes the Serpico Slim Polarized Sunglasses. These shades are identical in form and function to the original Serpico. These shades were slimmed down to accommodate smaller faces. With its polarized l... Read More
{"boxId":"compare4ea618d9b3f12a3583724f61f7", "id":"270714P"}
The RC Medallion Plus is style and simplicity at its best. Constructed from a Hi-Grip Urethane, the Medallion Plus offers superior roll and a little bit of flash all in one. Simply put the Medallion Plus Wheels are Super high grip urethane roller skate... Read More
{"boxId":"comparef8cea11dad4813c89a4a178471", "id":"147096P"}
The Smith Rockford Polarized is has a classic aviator frame with a medium sized frame. Adjustable Nose Pads allow you to fine tune the fit for total comfort. The Stainless Steel Frame is very durable and stylish, as you look at your co-piolet and say... Read More
{"boxId":"compare26569edd5ea516096c4c1bc7c9", "id":"435859P"}
Talk to me Goose. The Smith Rockford Slim Polarized is a great aviator fame with a scaled down size for the fighter pilots who have a smaller frame and face size. The Stainless Steel Frame is very durable, and adjustable nose pads provide a tailored ... Read More
{"boxId":"comparea59fa9c20e5b08deec12262d34", "id":"435861P"}


text:
Wow, this cat sure is lucky that her coat matches her eyes! You don't really get do-overs with that one…
Tip: Creating a profile and avatar takes just a minute and is a great way to participate in Catster's community of people who are passionate about cats.
Our Most-Commented Stories
Copyright © Lumina Media, LLC. All rights reserved.


text:
Astaro Corporation, a leading network security vendor, recently announced the launch of the Essential Firewall edition of its flagship security solution Astaro Security Gateway (ASG), available for free to all organizations worldwide. The Essential Firewall edition is claimed to include all the necessary functionality that all organizations need to secure their networks and operate a […]Read More
Posts tagged Astaro Security Gateway
Why Email Encryption is Critical
We've all heard the admonition a couple of times to secure our confidential data before sending it as an email attachment. Even a simple password-protected zip file is still better than nothing. On the high end, email encryption using tools like PGP, or the one included with Astaro Security Gateway will insure that your email […]Read More


text:
To protect my clients and remove uncertainty from their pursuits.
Set up a confidential, free and no-obligation consultation now
My reason to become an attorney was simple - I wanted to help people navigate the legal system, whether it was in business or the criminal justice system. I was (and still am) an entrepreneur before I became an attorney, and I have been a part of over a half dozen startups, both large and small. These experiences gave me a wealth of real-world experiences that I bring to my practice. I am also passionate about the criminal justice system, and many of the common issues surrounding the system, and want to help my clients go through this process with as minor of an impact on their lives as possible. Everyone makes mistakes in life, and I don't believe that these mistakes should be with people their whole lives - my goal is to help my clients through this process.
The choice of a lawyer is an important decision and should not be based solely upon advertisements.The information presented on HaakeLaw.com is of a general nature and is not intended as legal advice nor is it an agreement for legal representation. Contact the Haake Law Firm for case specific answers and/or exceptions. Use of this website does not create an attorney client relationship. Neither the Supreme Court of Missouri nor The Missouri Bar approve or review certifying organizations or specialist designations.


text:
The History of IRC
IRC (Internet Relay Chat) was invented in 1988. The colleges rapidly bent on and the babble spread.
Two of the oldest and better IRC networks are DALnet and EFnet and are still operating today. They
are networks of hundreds of servers all alive together, with bags of babble apartment and people.
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really put the time into it. Advances accept been made, and now we can accompany the apple of IRC babble and
webcams anon to you from web pages.
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chosen to use a webcam to enhance their cam babble experience, a webcam is not all-important to adore the
chat or see the webcams on a lot of sites. Some will even acquiesce you to add your webcam. It's all chargeless if
you acquisition the appropriate sites.
No amount if you are new to babble and webcams, or an accomplished IRC user, you can acquisition a place. Nearly
all altercation capacity are acceptable in a lot of webcams chat.
A lot of the users accept ICQ, AIM and/or Yahoo Messenger installed to accompaniment the reside camchat. They
may even accept reside video and articulation webcam chat.
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With IE and Netscape this is already on by absence unless you accept angry it off. If you are application a
popup blocker, accomplish abiding it is angry off. You may aswell babble with a angle abandoned IRC Client, such as
mIRC or pIRCh.
Thanks for reading,


text:
Bearing 5200, GPZ
- The bearing 5200 will be sent only after a detailed professional technical control.
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text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"John C. Turnbull" <ozem...@ozemail.com.au>
|RE: Returning multiple rows from a procedure call
|Wed, 01 Aug 2007 07:25:32 GMT
Further to this, I have looked at the method getArray() but it generates a not supported exception. I have also looked at using the JDBC type JAVA_OBJECT and then using it as an array but I am unable to get past syntax errors when declaring the procedure with an OUT parameter of that type - it just doesn't seem to recognise JAVA_OBJECT or OTHER which I found in the Derby reference manual. Can anyone help me fetch multiple rows from a procedure call? Thanks, John From: John C. Turnbull [mailto:email@example.com] Sent: Wednesday, 1 August 2007 11:08 To: 'Derby Discussion' Subject: Returning multiple rows from a procedure call I am a bit confused... I would like to have a procedure "return" multiple rows but I can't see any way to achieve this as the only methods available to process out parameters do not allow the specification of a row number. So is it possible to have a Java method that invokes a procedure and then process the multiple rows it generates? If so how? Thanks, John C. Turnbull
|View raw message


text:
Melanosomes are subcellular organelles in which melanin pigments are synthesized and stored within skin melanocytes and pigment cells of the eye. Within melanosomes, newly synthesized melanins polymerize on a sheet-like matrix of proteinaceous fibers that are largely composed of a single protein, PMEL. PMEL fibrils have biophysical features of amyloids like those associated with neurodegeneration in Alzheimer and Parkinson Diseases, but PMEL fibrils are non-pathogenic and serve a physiological role in pigmentation. By assessing how PMEL fibrils form within melanosome precursors and how their formation differs from that of pathogenic amyloid fibrils, we may better understand how to divert pathological amyloid into non-pathological forms. This proposal aims to (1) define molecular mechanisms that regulate physiological PMEL amyloid formation, and (2) test whether mutations that alter PMEL amyloid properties confer toxicity. Whereas pathological amyloids form slowly in solution, in vitro PMEL rapidly polymerizes into amyloid upon dilution out of denaturant, suggesting that the rate of fibril formation might be critical to distinguish functiona from pathological amyloid. How fibril formation is regulated in vivo is not known. Our preliminary data show that mature forms of PMEL just prior to fibril formation are disulfide bonded to a protein that we are in the midst of identifying. Mutations that interfere with this association hav no effect on early PMEL folding, processing or trafficking to endosomal compartments, but ablate the ability of PMEL to polymerize into amyloid fibrils in cells. This association thus likel impacts conformational changes required for amyloid formation. Preliminary data also show that fibrils begin to form in endosomes in association with intralumenal membrane vesicles (ILVs), suggesting that the ILVs themselves participate in amyloid biogenesis. Here, we will test the role of each of these factors in PMEL amyloid formation, and thereby begin to define the molecular mechanisms underlying the conformational change that converts PMEL from a soluble protein into physiological amyloid. Mice that lack a functional Pmel gene generate melanosomes without fibrils but have only modest defects in skin pigmentation. By contrast, several animal models bearing PMEL orthologues with dominant mutations are severely hypopigmented;human PMEL mutants bearing these mutations generate fibrils that have distinct morphological and functional properties and that inhibit melanogenesis in cultured cells. We hypothesize that the mutations render functional PMEL amyloid toxic to melanocytes. We will test this hypothesis in Aim 3 by expressing the mutant PMEL forms in mice and assessing their effect on pigment cell viability.
Our Specific Aims are: 1. To determine how a disulfide bonded associated protein facilitates PMEL amyloid formation;2. To test whether PMEL amyloid formation is seeded by endosomal membranes;and 3. To test whether dominant mutations in PMEL generate toxic amyloid in pigment cells.
Within melanosomes - subcellular organelles of pigment cells of the eye and skin - melanin pigments deposit on fibrils that resemble amyloid, a protein fold normally associated with neurodegeneration (as in Alzheimer and Parkinson Diseases). However, the melanosome fibrils are functional and not pathological. The goal of this proposal is to understand how functional PMEL amyloid forms and whether genetic alterations in its formation generate toxic amyloid. If successful, our results might explain novel forms of pigment loss and lead to novel approaches to detoxify pathological amyloid formation in neurodegenerative diseases and other amyloidoses.
|Theos, Alexander C; Watt, Brenda; Harper, Dawn C et al. (2013) The PKD domain distinguishes the trafficking and amyloidogenic properties of the pigment cell protein PMEL and its homologue GPNMB. Pigment Cell Melanoma Res 26:470-86
|Marks, Michael S; Heijnen, Harry F G; Raposo, Graca (2013) Lysosome-related organelles: unusual compartments become mainstream. Curr Opin Cell Biol 25:495-505
|Watt, Brenda; van Niel, Guillaume; Raposo, Graca et al. (2013) PMEL: a pigment cell-specific model for functional amyloid formation. Pigment Cell Melanoma Res 26:300-15
|Rochin, Leila; Hurbain, Ilse; Serneels, Lutgarde et al. (2013) BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells. Proc Natl Acad Sci U S A 110:10658-63
|Sitaram, Anand; Marks, Michael S (2012) Mechanisms of protein delivery to melanosomes in pigment cells. Physiology (Bethesda) 27:85-99
|Watt, Brenda; Tenza, Daniele; Lemmon, Mark A et al. (2011) Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic. PLoS Genet 7:e1002286
|Hellstrom, Anders R; Watt, Brenda; Fard, Shahrzad Shirazi et al. (2011) Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation. PLoS Genet 7:e1002285
|van Niel, Guillaume; Charrin, Stephanie; Simoes, Sabrina et al. (2011) The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis. Dev Cell 21:708-21
|Watt, Brenda; van Niel, Guillaume; Fowler, Douglas M et al. (2009) N-terminal domains elicit formation of functional Pmel17 amyloid fibrils. J Biol Chem 284:35543-55
|Marks, Michael S (2009) Eating thyself toward the dark side? Pigment Cell Melanoma Res 22:251-2
Showing the most recent 10 out of 21 publications


text:
I am going on my first ever guided hunt in September in either unit 48 or 49. I am going with Rimrock Outfitting, Do any of you guys on here know of this bunch. The operator is Steve Rivera and seems like a good man on the phone. Any help will be great guys. Thank you......
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text:
Print OR Send Commands from RDP Server to Client by using Remote Desktop Services Virtual Channels
- Status Closed
- Budget $250 - $750 USD
- Total Bids 14
Project Description
We have an application installed on Windows server 2008 r2 operating system. From different locations users (client computers) will connect to server by using RDP and use the application directly from server.
Each user (client computer) is going to have different type of Printers and it is really hard to maintain compatible drivers of each printer on the server
Currently I want to create an application to print directly to client printer from RDP server by using Remote Desktop Services Virtual Channels
We have to create 2 applications
1. Server Application: - This application will be installed on the server. This application will capture the Printed document and sends the document in binary format to client computer by using RDP Virtual Channel
2. Client Application: - This application will be installed on the client computer. This application will read the binary printed document data sent by "Server Application" and prints it on client computer default printer
To perform the above mentioned, I required a real time experienced .NET technical person in the same technology
The bidder has to create the applications in C#.NET OR [url removed, login to view] environment and configure them on our server environment. After successful verification, the source code should be provided with detail comments and explain to us in detail for further maintenance.Get free quotes for a project like this
Skills Required
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- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
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- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


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Guest reviews
Recent reviews
- All reviews
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Radisson Hotel Red Deer is a great choice for a stay in Red Deer. After getting a workout at the fitness center, you can grab a bite to eat at the restaurant or unwind with a drink at the bar/lounge. An indoor pool and a spa tub are other highlights, and rooms at this luxurious hotel offer up nice touches like pillowtop beds with Egyptian cotton sheets.


text:
A 'scandalous' chart
In 2006, Planned Parenthood performed more prevention services and cancer screenings than abortions, but in 2013, there were more abortions.
— PolitiFact National
on Thursday, October 1st, 2015
When call made, those 'getting killed' already dead
"We had people that were getting killed (in Benghazi), we had people who are willing to risk their lives to go save them, and somebody told them to stand down."
— PolitiFact National
on Tuesday, May 14th, 2013


text:
Latest Likes For LalaJJB
LalaJJB 3,154 Views
Joined Feb 7, '09. Posts: 95 (25% Liked) Likes: 93
Oct 15 '16
I had a patient just the other night bring a welcome mat and put it at the entrance of the room. Not even kidding.
Feb 29 '16
Your "rambling" makes me think you'd be a GREAT ER nurse. I'm a lot like you. I dont go out of my way for small talk, in fact i hate it more than anything. I found that ER nursing is what i was born to do because of that fact. i like to keep busy and i like to learn a lot...and most importantly, i dont like seeing the same person and changing their linens 3 days in a row. I know that may seem mean, but the thing I love about er nursing is the fact that I dont see the same people days in a row. I may see the same people...but most of the time it's because they're not sick, they just need their fix. I think ER nursing is perfect for you...


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7 Packing Tips to Save Time and Money
Gone are the days of loading up two "rollerboys" to take on a weekend excursion for you and your sweetie, or of dressing up as though you were embarking on a transatlantic journey on the Queen Mary. Here are our experts' surefire packing tips to make air travel as smooth as possible for yourself and your wallet.
1. Invest in a lightweight, microfiber, rolling carry-on: You'll be less inclined to overpack and less likely to go over the airline's allowed weight limit.
2. Pack one pair of shoes. Make the ones you wear on-board slip-ons, like loafers. The Transportation Security Administration (TSA) and your fellow passengers will thank you when it comes time to remove them.
3. Decant your shampoo. No need to pack whole bottles into your checked luggage. Buy inexpensive 3-ounce plastic bottles at your drugstore, and decant at home.
4. Stay away from salty snacks. The TSA now demands that any drinks brought on board be purchased after security check-in. Stick with fruit and cheese, and beware salty chips: you'll get dehydrated faster once aboard.
5. Pack one lightweight sweater suitable for day or night. Unless you're visiting the Arctic, there is no reason to travel with your entire winter wardrobe. Choose carefully, and pack lightweight clothes that can be layered.
6. Stick with one color scheme. Our experts uniformly agree that packing clothes that are black or white is the wisest way to travel. Everything will always match, black can be very dressy (or not), white always looks crisp, and you can brighten up outfits with a simple, lightweight scarf.
7. Avoid checking luggage. Packed intelligently, a carry-on rolling bag can work for trips as short as a weekend or as long as a month. Another reason to go this route: airlines are all starting to charge for checked luggage.
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Join Date:11-01-2010
Last Activity:10-22-2016 8:31 PM
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46 Likes on 29 Posts
- Raleigh
Posted in thread: Checking for off flavors while krausen present on 10-22-2016 at 02:39 AM
My gut is to go with your point on patience, only because in my experience, until a beer is
fully fermented (and often a bit past that) it can taste all sorts of strange or off-target,
only to come ou...
Posted in thread: Exploding Swing Tops on 10-03-2016 at 11:22 AM
I think the they are built for that pressure. Beer is sold in them, so I don't see why they
wouldn't be strong enough. Here, in local supermarkets, they sell unfiltered/unpasteurized
Hefeweizen with l...
Posted in thread: Exploding Swing Tops on 10-03-2016 at 10:27 AM
Correct me if I'm wrong, but I believe these types of bottles are not technically built to hold
the pressure necessary for bottle carbing, despite the fact that they often work out fine.
Posted in thread: What brewery are you visiting? on 09-30-2016 at 12:33 AM
Hitting Asheville NC this weekend. Will get to some of the standards...Sierra Nevada, Oskar
Blues, Green Man, French Broad, wicked weed. Wife is preggo aka the brewery tour driver!
Posted in thread: Should I bother with cold crash? on 09-30-2016 at 12:29 AM
I do a mix of the above. Cold crash all of my beers and add gelatin once it gets cold, then
rack to keg after 48hrs and put it on gas. Crystal clear beer almost immediately.
Posted in thread: Confession Time on 09-28-2016 at 11:27 AM
Bless me fellow brewers, for I have sinned;I never boil the water I use to re-hydrate dry
yeast.And my carboy brush is covered with rust....mmmmm iron.
Posted in thread: Car boy plug? on 09-27-2016 at 12:43 PM
It's for secondary fermentation so do I need a air lock or could I just cover it with tin foilI
would certainly try to use an airlock either way, but definitely so if it's in secondary--
most/all of t...
Posted in thread: Original Gravity on 09-26-2016 at 03:08 PM
Hard to say what could've caused it based on the info, but I doubt it will be a huge deal. One
thing I would do (if you don't already) is document and track your OGs over a series of batches
so you ca...
Posted in thread: Brew days wear me out! on 09-24-2016 at 07:59 AM
Prep ahead of time, clean as you go, and when lifting, use your legs, not your back! Smooth
sailing on brew days.
Posted in thread: Propane Burner for new AG setup on 09-21-2016 at 11:41 AM
I've been using an SQ-14 for a few years now, boiling 7 or so gallons for 5 gallon batches.
It's worked out great so far - stable, easy to control flame, and pretty efficient as far as I
can tell.